LOCUS       DATDMW010000001         7901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1992199, whole genome shotgun sequence.
ACCESSION   DATDMW010000001 DATDMW010000000
VERSION     DATDMW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992199"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VI319_00005"
     CDS             <1..1014
                     /locus_tag="VI319_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6862257.1"
                     /translation="FQRFAESWKLPVGCAFRFQDVFDNRHPCYAGDVGIGINPKLAQR
                     VRDADLLLVVGARLGEMTTGAYTLIEPPRPKQKLVHVHAGAEELGRVYQADLLINSGM
                     PQIAGALAGVALDGSRWRSWRESARADYLEWTRPAAIPGRLQLAEVVAWLRERLPEDA
                     IIANGAGNFAGWIGRYYRYPRWRTQVAPTNGSMGYGVPAGIAAKIAHPDRVVLSFNGD
                     GDFLMNGQELATAVQYRVPVIFFVVNNAMYGTIRMHQEREYPARVYGTELLNPDFPAL
                     ARAYGAYGETVEDTAGFAPAFERALRETEGGKPAVLELRIDPQAITTTTTLDAIRARA
                     LKG"
     gene            complement(1024..3990)
                     /locus_tag="VI319_00010"
     CDS             complement(1024..3990)
                     /locus_tag="VI319_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY6862258.1"
                     /translation="MSPRNLRLLFALFTVSGFTGLIYESVWSHYLRLFLGAAAFAQSF
                     VLAAFMGGMALGAWGAGRWSARLRAPLAAYAAVEALVGLLALAFHEVYVVTTQFSLDH
                     VIPALGSPGSVEIYKYSVCALLIVPQTILLGATFPLLSGAVIRSDPDASGHHLAMLYF
                     TNSIGAALGALAAAFLLLGWLGMPGTMRLAGVLNLMLAAIVMQVARQGEPAARPAVPR
                     AGAAPVGKRLVVLFLAAAFFTGAASFIYEIAWVRLLALVLGGSFHAFELMLSAFITGL
                     ALGGLWIRRRIDRIARPVYFAGAVQVLMGLAAVATVFVYSRTFDGMAWIMATLQRNAG
                     GYVLFNVLSHGIAFAVMLPATFLAGMTLPLFTHVLLRGGYGERAIGQVYASNTLGAIV
                     GVVVIVHVMIPEIGVKLSLVGGAAVDIVLGAWLFRYSEVAWRPVHSFASLILGLLIAA
                     LTGRAEVLSPDRLASGVYRYGLAEPPETRVFFYRDGKTASVAMREHGSMVSIVTNGKP
                     DAAIQLDPTKEPGPDEYTMTLTAALPLLIKPDARTYANIGWGSGLTVETLMSHSGPRE
                     VDTIEIEPAMVAGARAFYPRVLRAYRDPRSHVFFEDAKSYFARHGKRYDVIISEPSNP
                     WVNGVASLFTTEFYHEARRYLAPGGLFVQWLQLYELNDALLGSMVAALGENFSDYEVY
                     ESGAGDIILVAVAEGTVPRPQPLPANEKVFMPMLARVGITRLEHVTLRSLGTKRTIHN
                     LLAPLASPVNSDFRPTVQIEAARARFEGRLAGGIRSLAEAPLPIAEMLDGEKRVYLSE
                     PAGGSEKAVPLLAQSLALRLARGLLQKGPDALRPGNEQVMVPLLMLESPGVLCGREPA
                     KAVVDQLHRVAEVTLNRLPPELLRRLWIRGEWLGCPVEKLAPEVRRRFALYGAIAARD
                     AKQMLERASAILGEPVEDSKEGREWRRFVLLTAMLGAHASGRTDEAGRLWLAHRDEIN
                     TTEDVPAYLIYLVNHD"
     gene            complement(4089..4817)
                     /locus_tag="VI319_00015"
     CDS             complement(4089..4817)
                     /locus_tag="VI319_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY6862259.1"
                     /translation="MISIRNVSKWYGDFRVLTECSTEVKKGEVVVVCGPSGSGKSTLI
                     KCVNALEPFQQGEVLVDGVSVGDPRTDLPRLRSRIGMVFQNFELFPHLSVTQNLTLAQ
                     VKVLRRSEPEALERGLKMLDRVGLAAHRDKYPGQLSGGQQQRVAIARALAMDPVAMLF
                     DEPTSALDPEMINEVLDVMVQLARDGMTMMCVTHEMGFARKVAHRVVFMDGGRIVEDA
                     PTEAFFGQPRSPRAQQFLSKILRH"
     gene            complement(4834..5526)
                     /locus_tag="VI319_00020"
     CDS             complement(4834..5526)
                     /locus_tag="VI319_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY6862260.1"
                     /translation="MFSGYDFDVIERSLPYLFREGMTFTVTLTLLAMTGGIVFGTLLA
                     MMRLSSRRPAAAAAGAYVNLMRSVPLVLVIFWFFFLVPYIGAWVIRAGEPVKVGAFAS
                     SVITFTMFEAAYYCEIMRAGIQSVPRGQAWAGYALGLSYGQTMARVVLPQAFRNMLPV
                     LLTQSIILFQDTSLVYVISATDFLGAATKIANRDYRLVEMYTFVAVVYFAVSYGLSML
                     VRRMQRRLAVVR"
     gene            complement(5531..6289)
                     /locus_tag="VI319_00025"
     CDS             complement(5531..6289)
                     /locus_tag="VI319_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY6862261.1"
                     /translation="MGYHWHWGVLLEPEPGGTGTYLHYLCVGLGWTLATALAAWAIAL
                     TVGAAVGTVRTTPARWAVRLGDAYVEIFRNVPLIVQMFLWYFVVPELLPQAAGDWIKQ
                     LPPPWGMYFPAVLCLGVFTSVRVAEQVRAGIESLPRGQRLAGTALGLTLPQTYRHVLL
                     PMAFRIVLPPLTSEFMNVIKNSSVALTIGLLELTGRARAMQEFSFSAFEAFAAATVIY
                     LLTNLVVVLAMRALERKVRVPGLIAAGTAAQAGH"
     gene            complement(6295..7200)
                     /locus_tag="VI319_00030"
     CDS             complement(6295..7200)
                     /locus_tag="VI319_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6862262.1"
                     /translation="MIRLVRPVLALLALLAVAAPAAAQEGTLKKIKDSGAITLGHRDA
                     SIPFSYYDDQQRPVGYALDLCLKIVDAVKAELKLPKLEVKYQLVTSANRIPLMANGTI
                     DLECGSTTNNLEREKQVAFTITHFVTANRWVARKSAKIRSLADLKGKTIVSTAGTTNI
                     KQITEINAAQNLGMHIISANGHPEAFQMVETGRAVAFVMDDILLYSLAAQSRRPADYD
                     ISQTALSVEPYGIMLRRDDPAFKKVVDAAMVRVYKSGEINRIYDKWFLKPIPPRGLSL
                     NVPMSAQFKKVVAHPTDSGDPEAYK"
     gene            7264..>7901
                     /locus_tag="VI319_00035"
     CDS             7264..>7901
                     /locus_tag="VI319_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY6862263.1"
                     /translation="MIQAPTRVEHDLLGERAVPADAYYGIHTLRALENFPITGTSISI
                     YPELVRALACVKQAATLANNALGLLPDAKADAITRACEEIREGELLDQFVVDVIQGGA
                     GTSTNMNANEVIANRGLELMGHARGDYRLLHPNEDVNMSQSTNDVYPTSLRLASYVGL
                     MRLIDSMAYLRSAFEAKAEEFRHVLKMGRTQLQDAVPMTLGQEFSTYAVMLG"
BASE COUNT         1317 a         2683 c         2759 g         1142 t
ORIGIN      
        1 ttccagcgct tcgccgagag ctggaagctg ccggtgggct gcgccttccg cttccaggac
       61 gtgttcgaca accggcaccc gtgctacgcg ggcgacgtcg gcatcggcat caacccgaag
      121 ctcgcgcagc gcgtgcgcga cgccgacctg ctgctcgtcg tcggcgcgcg cctcggcgag
      181 atgacgaccg gcgcctacac gctgattgag ccgccgcggc cgaagcagaa gctcgtgcac
      241 gtgcacgcgg gcgccgagga gctgggccgc gtgtaccagg cggacctgct gatcaacagc
      301 ggcatgccgc agatcgccgg cgcgctcgcg ggcgtcgcgc tcgacggctc gcgctggcga
      361 tcgtggcgcg agtcggcgcg cgccgactac ctcgaatgga cccgccccgc ggcgatcccg
      421 ggacgcctgc agttggccga ggtcgtggcg tggctgcgcg agcgcctgcc cgaggacgcg
      481 atcatcgcca acggcgccgg caacttcgcg ggctggatcg gccgctacta ccgctatccg
      541 cgctggcgca cgcaggtcgc gccgaccaac ggctcgatgg gctacggcgt cccggcgggc
      601 atcgcggcca agatcgcgca ccccgatcgc gtcgtgctct ccttcaacgg cgacggcgac
      661 ttcctgatga acgggcagga gctcgcgacc gccgtgcagt accgcgtgcc cgtgattttc
      721 ttcgtggtca acaacgccat gtacggcacg atccgcatgc accaggagcg cgagtacccc
      781 gcgcgggtct acggcacgga gctcctcaat cccgacttcc ccgcgctcgc gcgcgcctac
      841 ggcgcgtacg gggagaccgt cgaggacacc gcgggcttcg cgcccgcgtt cgagcgcgcg
      901 ctgcgcgaga ccgagggcgg caagccggcg gtcctcgagc tgcgcatcga cccgcaggcg
      961 atcacgacga cgacgacgct cgacgcgatc cgggcgcgcg cgctgaaggg ctagcgcgcc
     1021 cgctcagtcg tggttgacga gatagatgag gtacgccggg acgtcttcgg tcgtgttgat
     1081 ctcgtcgcgg tgcgccagcc acaggcgccc ggcctcgtcg gtgcggccgg acgcatgcgc
     1141 gcccagcatc gccgtgagca gcacgaagcg ccgccattcc ctgccttcct tgctgtcctc
     1201 gacgggctcg cccaatatcg cgctcgcccg ctcgagcatc tgcttcgcgt ctcgggcggc
     1261 gatcgcgccg taaagcgcga atcgacgcct gacctcgggc gcgagcttct ccaccgggca
     1321 gccgagccac tcgccgcgga tccacaggcg gcgcagcagc tcgggcggga gacggttcag
     1381 cgtgacctcg gcgacgcggt ggagctggtc caccacggcc ttcgccggct ccctgccgca
     1441 caggacgccg ggcgactcga gcatgaggag cgggaccatc acctgctcgt tgcccggacg
     1501 caaggcgtct ggacccttct gcagcagccc gcgcgcgagg cgcagcgcga gcgactgcgc
     1561 gagcagcggc acggccttct ccgatccacc cgcgggctcc gacaggtaca cgcgcttctc
     1621 gccgtccagc atctcggcga tcggcagcgg cgcttccgcg agcgaccgga tgccgcccgc
     1681 cagccgccct tcgaagcgcg cacgtgccgc ttcgatctgc accgtcggac ggaaatcgga
     1741 gttgaccggc gaggcgagcg gagcgagcag gttgtggatc gtgcgcttgg tgccgaggct
     1801 gcgcagcgtg acgtgctcga gccgggtgat gccgacccgg gcgagcatgg gcatgaacac
     1861 cttctcgttc gcgggcaggg gctgcgggcg cggcaccgtc ccctcggcga ccgcgacgag
     1921 gatgatgtcg ccggccccgg attcgtagac ctcgtagtcc gagaagttct cgcccagcgc
     1981 ggcgaccatc gatccgagca gcgcgtcgtt cagctcgtag agctgcagcc actgcacgaa
     2041 caggcctccg ggcgcgaggt aacgcctggc ctcgtggtag aactccgtcg tgaacaggct
     2101 ggcgacgccg ttcacccagg ggttcgatgg ctccgagatg atgacgtcgt agcgcttgcc
     2161 gtgccgcgcg aaatagctct tcgcgtcctc gaagaacacg tggctgcgcg ggtcgcggta
     2221 ggcgcgcagc acgcgcggat aaaaggcacg cgcgccggcc accatcgcgg gctcgatctc
     2281 gatcgtgtcg acctcgcgtg gcccgctgtg cgacatcagc gtttccacgg tcaggcccga
     2341 gccccatccg atgttcgcgt aggtgcgcgc gtccggcttg atcaggagcg gcagcgcggc
     2401 cgtcagggtc atcgtgtact cgtcgggccc cggctccttg gtggggtcga gttggatcgc
     2461 cgcgtcgggc tttccgttcg tcacgatcga gaccatgctg ccgtgctcgc gcatcgccac
     2521 ggatgccgtc ttgccgtcac ggtagaagaa cacccgcgtc tcgggcggct cggccagccc
     2581 gtagcggtaa acgccggaag ccagccggtc cgggctgagc acctcggcgc gcccggtgag
     2641 cgcggcgatc agcaagccga ggatgaggga ggcgaaagaa tgcacgggtc gccatgcgac
     2701 ctcggagtac cggaacagcc aggcgccgag gacgatgtcc accgccgcgc cgccgaccag
     2761 gctgagcttc acgccgatct ccgggatcat gacgtggacg atgacgacca cgccgacgat
     2821 tgcgccgagc gtgttcgacg cgtacacctg cccgatcgcg cgctcgccgt agccgccgcg
     2881 aagcagcaca tgcgtgaaca ggggcagcgt cattcccgcg aggaacgtcg ccggcagcat
     2941 gacggcgaac gcgatcccgt ggctgaggac gttgaacagg acgtagccgc ccgcgttccg
     3001 ctggagcgtc gccatgatcc acgccatccc gtcgaacgtc cggctgtaga cgaagacggt
     3061 ggcgaccgcg gcgagcccca tgaggacctg cacggcaccc gcgaaataga ccgggcgggc
     3121 gatgcggtcg atgcgcctgc gtatccacag tccgccgagc gcgagcccgg tgatgaacgc
     3181 ggacagcatc agctcgaacg cgtggaacga cccaccgagg acgagcgcga gcagccgcac
     3241 ccaggcgatc tcgtagatga acgacgccgc gccggtgaag aacgcggcgg cgaggaacag
     3301 cacgacgagc cgcttgccga cgggggcggc gcccgcgcgc ggtacggccg gccgcgccgc
     3361 gggctcgccc tggcgcgcga cctgcatcac gatcgcggcg agcatcaggt tgagcacgcc
     3421 ggcgagccgc atcgttcccg gcatgccgag ccagccgagc agcaggaacg cggccgcgag
     3481 cgcaccgagc gcggcgccga tggagttggt gaagtacagc atcgcgagat ggtggccgct
     3541 cgcgtccgga tcgctccgga tcacggcgcc gctgagcagg gggaacgtgg cgcccagcag
     3601 gatggtctgc ggaacgatca gcagcgcgca caccgagtac ttgtagatct cgaccgaccc
     3661 gggcgacccg agcgcgggga tcacgtgatc cagcgagaac tgcgtcgtga cgacgtagac
     3721 ctcgtggaac gccagggcca ggagcccgac gagcgcctcg accgcggcgt atgcggcgag
     3781 cggcgcgcgc aggcgcgcgc tccagcggcc cgcgccccac gcgccgagcg ccattccgcc
     3841 catgaacgcg gcgagcacga aggattgcgc gaacgcggct gcgccgagga agaggcggag
     3901 gtagtgcgac cagaccgact cgtagatcag cccggtgaag cccgagaccg tgaagagcgc
     3961 gaaaagaagc ctgagattcc gtggcgacat gtcgttgtcg tatcctcccg cgcgcagtgt
     4021 agcctgcccc ggggcatctc gcggccggaa tttttcacgc cgattcgcgc gcggcggccc
     4081 gcgccgagtc agtggcgcag gatcttggag aggaactgct gcgcgcgcgg cgagcgcggc
     4141 tgaccgaaga aggcctcggt gggcgcgtcc tcgacgatgc ggccgccgtc catgaacacg
     4201 acgcggtgtg cgaccttgcg cgcgaatccc atctcgtggg tcacgcacat catcgtcatg
     4261 ccgtcgcggg cgagctgcac catcacgtcg agcacctcgt tgatcatctc ggggtcgagc
     4321 gccgaggtcg gctcgtcgaa cagcatggcg accgggtcca tcgcgagcgc gcgcgcgatg
     4381 gcgacacgct gctgctggcc gccggagagc tggcccgggt acttgtccct gtgcgcggcg
     4441 agcccgacgc gatcgagcat cttcagtccg cgctcgagcg cctcgggctc gctccggcgc
     4501 agcaccttga cctgcgcgag cgtgaggttc tgcgtgaccg agagatgggg aaagagctcg
     4561 aagttctgga acaccatgcc gatgcgcgag cgaaggcgcg ggagatcggt gcgcgggtcg
     4621 ccgacggaca cgccgtcgac caggacttcg ccctgctgga aaggctcgag cgcgttcacg
     4681 catttgatga gcgtcgactt gcccgacccc gacgggccgc acacgaccac cacctcgccc
     4741 ttcttcacct cggtcgagca ttcggtgagg acgcggaagt cgccgtacca cttgctcacg
     4801 ttccggatcg agatcattgc gctccgtgga gagtcagcgg acgacggcca gccgacgctg
     4861 catgcggcgc accagcatcg acagtccgta cgagaccgca aagtacacga cggcgacgaa
     4921 ggtgtacatc tccaccagcc ggtagtcgcg attggcgatc ttggtcgccg cgccgaggaa
     4981 gtcggtcgcc gagatcacgt acacgagcga ggtgtcctgg aacaggatga tgctctgcgt
     5041 gagcagcacc ggcagcatgt tgcggaaggc ctgcggcagg accacgcgcg ccatcgtctg
     5101 cccgtagctc aggccgagcg cgtagccggc ccaggcctgc ccgcgcggga ccgactggat
     5161 ccccgcgcgc atgatctcgc agtaatacgc ggcctcgaac atcgtgaacg tgatgacgct
     5221 cgatgcgaag gcgcccacct tgaccggctc gcccgcgcgg atgacccagg cgccgatgta
     5281 cggcaccagg aagaagaacc agaagatgac gagcaccagg ggcaccgagc gcatcaggtt
     5341 gacgtacgcg ccggcggccg ccgccgcggg gcgccggctc gagagccgca tcatcgcgag
     5401 cagggttccg aacacgatgc cgcccgtcat cgcgagcagg gtcagcgtga cggtgaacgt
     5461 catcccctcg cggaagagat acggaagcga gcgctcgatg acgtcgaagt cgtagcccga
     5521 gaacatcggc tcagtgcccg gcctgcgccg ccgtgcccgc cgcgatcagg cccgggacgc
     5581 ggacctttcg ttccagcgcg cgcatcgcga gcacgacgac gaggttggtg aggagataga
     5641 tcacggtggc ggcggcgaag gcctcgaacg cgctgaagct gaactcctgc atggcgcgcg
     5701 cgcggccggt gagctcgagc aggccgatcg tgagcgcgac cgacgagttc ttgatcacgt
     5761 tcatgaactc ggaggtgagc ggcggcagca cgatgcggaa cgccatcggc aggagcacgt
     5821 ggcggtacgt ttgcggaagc gtcagcccga gcgcggtgcc cgcgagccgc tggccgcgcg
     5881 gcagcgactc gatcccggcc cgcacctgct cggcgacgcg caccgaggtg aagacgccga
     5941 ggcacagcac ggcggggaag tacattcccc atggcggggg cagctgcttg atccagtcgc
     6001 cggcggcctg cggcagcagc tccgggacga cgaagtacca caggaacatc tgcacgatca
     6061 gcgggacgtt cctgaatatc tcgacgtaag cgtcgccgag ccggaccgcc caccgcgccg
     6121 gcgtcgtgcg caccgtgccg accgcggcgc cgaccgtcag tgcgatcgcc caggcggcga
     6181 gcgcggtcgc gagcgtccag cccaggccga cgcaaaggta gtgcaggtag gtgccggtgc
     6241 cgcccggctc gggctcgagc agaacgcccc agtgccagtg atagcccacc taagctactt
     6301 gtacgcttcc gggtcgccgg aatcggtcgg gtgcgcgacc accttcttga actgcgcgct
     6361 catggggacg ttgaggctga gcccgcgcgg cgggatcggc ttgaggaacc atttgtcgta
     6421 gatgcggttg atctcgccgc tcttgtagac gcggaccatg gcggcgtcga ccaccttctt
     6481 gaacgcgggg tcgtcgcgtc gcagcatgat gccgtagggc tcgaccgaga gcgccgtctg
     6541 cgaaatatcg tagtccgcgg gccggcgcga ctgcgcggcc aggctgtaga gcaggatgtc
     6601 gtccatgacg aaggcgaccg cgcggcccgt ctcgaccatc tggaacgcct ccgggtggcc
     6661 gttcgccgag atgatgtgca tgccgaggtt ctgcgcggcg ttgatctcgg tgatctgctt
     6721 gatgttggtg gtcccggcgg tcgagacgat cgtcttgccc ttcaggtcgg cgagcgagcg
     6781 gatcttcgcc gacttccgcg cgacccagcg gttggcggtg acgaagtgcg tgatcgtgaa
     6841 cgcgacctgc ttctcgcgct cgaggttgtt cgtggtcgag ccgcactcga gatcgatcgt
     6901 gccgttcgcc atcagcggga tgcggttggc cgaggtgacg agctggtact tcacctcgag
     6961 cttcggcagc ttcagctccg ccttcacggc gtcgacgatc ttcaggcaca ggtcgagcgc
     7021 gtagccgacc ggccgctgct ggtcgtcgta gtaggagaag gggatcgagg cgtcgcggtg
     7081 cccgagggtg atggcgccgc tgtccttgat cttcttgagc gtgccctcct gggccgcggc
     7141 cggtgcggcg acggcgagga gggcgaggag ggcgagcact ggtcggacaa gtcgtatcat
     7201 gtcggtctcc cgggcgaggc tgcacgattc tagcccagcg gccgaaccag cgaaggaagc
     7261 cccatgatcc aggccccgac acgggtcgag cacgatctgc ttggcgagcg cgccgtcccc
     7321 gccgacgcct actatggcat ccacacgctg cgcgcgctgg agaacttccc gatcaccggg
     7381 acctcgatct cgatctaccc cgagctggtg cgcgcgctcg cctgcgtgaa gcaggcggcc
     7441 acgctcgcga acaacgcgct cgggctcctg cccgacgcca aggccgacgc gatcacgcgc
     7501 gcctgcgagg agatccgcga gggcgagctg ctcgaccaat tcgtcgtcga cgtgatccag
     7561 ggcggcgccg gcacctcgac caacatgaac gcgaacgagg tgatcgccaa ccgcggcctg
     7621 gagctgatgg ggcacgcccg cggcgactac cgcctgctgc atccgaacga agacgtcaac
     7681 atgagtcagt cgaccaacga cgtctatccg acttcgctac gcttggcgag ctacgtcggc
     7741 ctgatgcgcc tgatcgactc catggcctat ctgcgcagcg ccttcgaagc caaggccgag
     7801 gagttccgcc acgtcctgaa gatgggccgc acacaattgc aggacgccgt accgatgacg
     7861 ctggggcaag agttctccac ctacgccgtg atgctcggcg a
//
LOCUS       DATDMW010000002         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_498435, whole genome shotgun sequence.
ACCESSION   DATDMW010000002 DATDMW010000000
VERSION     DATDMW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498435"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VI319_00040"
     CDS             complement(<1..360)
                     /locus_tag="VI319_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6862264.1"
                     /translation="MKLYTFFRSSASFRVRIALNVKGIAYEAVGVSLAKAEHLQDAYR
                     TINAQALVPTLEDGGRLLGQSLAIMEYLDEVHGGPRLLPADPLDRHYVRAFSQIVACE
                     IHPLNNLRTLKYVRKTYG"
     gene            complement(368..628)
                     /locus_tag="VI319_00045"
     CDS             complement(368..628)
                     /locus_tag="VI319_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HEY6862265.1"
                     /translation="MSDIALNVLGFAAGVLTTVAFVPQVIKLRRTRSARDISLGMYTV
                     FTSGVALWLVYGIALGSMPIVVANVFTLALALAVLAMKVRYG"
     gene            complement(638..2281)
                     /locus_tag="VI319_00050"
     CDS             complement(638..2281)
                     /locus_tag="VI319_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6862266.1"
                     /translation="MAAAPDRYREVYDSFRWEVPARFNIAHACCRRHAGDAARVALLW
                     EDETGATSAWTYRQIQQAADRLSNALAALGVARGDRVAVVLPQRPETAIAHIALYQMG
                     AVAMPLSVLFGPDALEYRIGDSEAVAALVDPASLPNLAAVRDRLPALKHVIGVAGARE
                     SRVTPWETLLERASPDFAPVDTAADDPAILVYTSGTTGPPKGALKAHRAVIGNLPGFS
                     YSHDLFPQPGDVFWSPADWAWTGGLMDALLPTMYYGHPLVGYRGRFDPERALALMQKY
                     RVRNSFLFPTALKMIMKAVPRPRERHDLALRSIMSAGESVGETVFAWSREALGVTVNE
                     MFGQTEINYIVGNSHTLWPAEPGSIGRPYPGHRVACIDDAGNEVPTGDVGDIAAWDEG
                     DPVFFLGYWRNPQATKAKYTGRWCRTGDLARRDERGNFWYQGRSDDMFKSAGYRIGPS
                     EIESCLLKHPAVANCAVVGSPDPERTHLVKAFVVLTAGRSGSPELEAELQAQVRAKLA
                     PYEVPKEIEFIDALPMTTTGKVQRRVLREREVQRKRGSS"
     gene            complement(2331..>3161)
                     /locus_tag="VI319_00055"
     CDS             complement(2331..>3161)
                     /locus_tag="VI319_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6862267.1"
                     /translation="VSAPAQPQSEKPASAADAAKLDMLKLATELATPVRARPAPAKPR
                     ARTTAEPRKPARAGIDASTLAALKAQAPLEPAPGQIDRRASGTAHERAAAEFRRAVGL
                     VNQGRVAEGLDGLRAALKIDPGYDEVRQTLVALLIEAKQMDEAAAVLRAGLALEPSNT
                     KFAVVLARVMVEHNDVAGALALLRQHAAAGESSPDYHAFAAALYQRLERHGDAVAEYQ
                     AALKLAPNAGVWWVGLGISQQALNRPKDAVLAFQRAKDSGNLAPELAAFTDKRLKLLQ
                     "
BASE COUNT          440 a         1122 c         1123 g          476 t
ORIGIN      
        1 gccgtacgtc ttcctcacgt acttcagcgt gcgcaggttg ttgagcgggt ggatctcgca
       61 cgcgacgatc tgcgagaacg cgcgcacata gtgccggtcg agcggatcgg cgggcagcag
      121 gcgcggcccg ccgtggacct cgtcgaggta ctccatgatc gcgagcgact gcccgagcag
      181 ccgcccgccg tcctcgaggg tcggcaccag cgcctgcgcg ttgatcgtcc ggtacgcgtc
      241 ctgcaggtgc tcggccttgg ccaggctcac accgacggcc tcgtacgcga tgcccttgac
      301 gttgagcgcg atgcgcaccc ggaacgacgc cgacgagcgg aagaacgtat acagtttcat
      361 ggcactccta gccgtagcgc actttcatgg cgaggaccgc gagcgcgagc gccagcgtga
      421 agacgttcgc gacgacgatc ggcatcgagc cgagcgcgat gccgtagacc agccacagcg
      481 ccaccccgct cgtgaacact gtgtacattc cgagcgagat gtcccgggcc gaccgggtcc
      541 ggcggagctt gatcacctgc gggacgaagg cgacggtggt gaggacgccg gcggcgaagc
      601 ccagaacgtt gagcgcgata tcggacatgc cccgattcta ggacgagccg cgcttccgct
      661 gcacctcccg ctcgcgcagc acccgccgct gcaccttgcc ggtcgtggtc atgggcagtg
      721 cgtcgatgaa ctcgatctcc ttcgggacct cgtaaggcgc gagcttggcg cgcacctgcg
      781 cctgcaattc ggcctcgagc tcgggcgagc cgctccgtcc ggcggtgagc accacgaagg
      841 ccttcaccag gtgggtgcgc tcgggatcgg ggctgccgac cacggcgcaa ttcgccaccg
      901 ccgggtgctt cagcaggcag ctctcgatct cggaaggacc gatgcggtag ccggcgctct
      961 tgaacatgtc gtccgagcgg ccctggtacc agaagttgcc gcgctcgtcg cgccgcgcga
     1021 gatcgccggt gcggcaccag cggccggtgt acttggcctt cgtcgcctgc gggtttctcc
     1081 agtagccgag gaagaacacc gggtcgccct cgtcccatgc ggcgatgtcg ccgacgtcac
     1141 cggtcggtac ctcgttcccg gcgtcgtcga tgcaggcgac gcggtgcccg ggatacgggc
     1201 ggccgatcga tcccggctcg gccggccaca gcgtatgcga gttgccgacg atgtagttga
     1261 tctcggtctg cccgaacatc tcgttcaccg tcaccccgag cgcctcgcgg ctccacgcga
     1321 aaaccgtctc gcccaccgac tcgcccgcgc tcatgatcga gcgtagcgcg aggtcgtggc
     1381 gctcgcgcgg ccgcggcacg gccttcatga tcattttcag cgcggtcggg aacaggaagc
     1441 tgttgcgcac gcggtacttc tgcatcagcg cgagcgcgcg ctcgggatcg aagcgccccc
     1501 ggtagccgac gagcggatgg ccgtagtaca tcgtcggcag cagcgcgtcc atcaggccgc
     1561 ccgtccaggc ccagtcggcc ggcgaccaga acacgtcgcc cggctgcggg aacaggtcgt
     1621 gcgagtacga gaagcccggc aggttgccga tcaccgcgcg gtgcgccttc aacgcccctt
     1681 tcggcggtcc ggtcgtgccg ctcgtataca cgaggatcgc gggatcgtcg gccgcggtgt
     1741 cgaccggcgc aaagtccggc gaagcgcgct cgaggagagt ctcccacggg gtgacccgcg
     1801 attcgcgcgc gcccgcgacg ccgatcacgt gcttgagcgc gggcagccgg tcgcgcacgg
     1861 ccgcgaggtt cggcagcgac gccgggtcga cgagcgccgc gaccgcctcg ctgtccccga
     1921 tgcggtactc gagcgcgtcg gggccgaaca gcaccgagag cggcatggcg accgcgccca
     1981 tctggtacag cgcgatgtgc gcgatcgcgg tctcgggacg ctgcggcagg acgacggcga
     2041 cccggtcgcc gcgcgcgacg ccgagggcgg cgagcgcgtt cgagaggcgg tcggcggctt
     2101 gctggatctg ccggtaggtc caggccgacg tcgcgccggt ctcgtcctcc cagagcagcg
     2161 cgacgcgcgc ggcgtcgccg gcgtgccggc ggcagcacgc gtgcgcgatg ttgaagcgcg
     2221 ccgggacctc ccagcggaag ctgtcgtaga cctcgcggta gcggtccggc gcggcggcca
     2281 tgccttcctc cgcggcgaaa cttaccacgg aggccggcgc ggcgcgccgg ctactgcagg
     2341 agcttcaggc gcttgtcggt gaaggccgcg agctcgggcg cgagattccc ggagtccttc
     2401 gcgcgctgga acgccagcac cgcgtccttc ggtcggttca gcgcctgctg cgagatgccg
     2461 aggccgaccc accacacgcc cgcgttcggc gcgagcttga gcgcggcctg gtactcggcg
     2521 acggcgtcgc cgtggcgctc cagcctctgg tacagggcgg cggcgaacgc gtgatagtcc
     2581 ggactgcttt cgcccgctgc ggcatgctgc cgcaggagcg cgagcgcccc ggcgacgtcg
     2641 ttgtgctcca ccatcacgcg cgcgagcacc acggcgaatt tcgtgttgga gggctccagc
     2701 gcgaggccgg cgcgcagcac cgctgcggcc tcgtccatct gcttcgcttc gatgagcagc
     2761 gcgaccagcg tctggcggac ttcgtcgtag cccgggtcga tcttcagcgc cgcgcgcagg
     2821 ccatccaggc cctcggcgac gcgtccctgg ttcaccaggc ccaccgcgcg ccggaactcg
     2881 gccgccgcgc gctcgtgcgc cgtgccgctg gcgcgccggt cgatctgtcc cggggcaggc
     2941 tcgagcggcg cttgcgcctt gagcgcggcg agcgtgcttg catcaatgcc cgcgcgagcc
     3001 ggcttccgcg gctcggcggt cgtgcgcgca cggggcttcg ccggcgccgg cctggcccgc
     3061 accggcgtcg ccagctcggt cgcgagcttc agcatatcga gcttggcggc gtccgctgcg
     3121 gatgcgggct tctcggactg cggctgcgcc ggcgccgaga c
//
LOCUS       DATDMW010000003         5959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_623038, whole genome shotgun sequence.
ACCESSION   DATDMW010000003 DATDMW010000000
VERSION     DATDMW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623038"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VI319_00060"
     CDS             complement(<1..485)
                     /locus_tag="VI319_00060"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEY6862268.1"
                     /translation="MTRTRAAAAFAAGLALAGCVLLGGCAAGAASTSYYTLSPEAAPA
                     AGAPAGTNVRTASVWQVAIPEMVDRRALVVRTAPNRVEISDLHQWAEPLRFGIARTLA
                     ADIAARLGPGWAVVAGQPLGTSPEVRVFVDVQRFEAVAGRGVAVEALWSVRPAQGERR
                     EG"
     gene            complement(482..2125)
                     /locus_tag="VI319_00065"
     CDS             complement(482..2125)
                     /locus_tag="VI319_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY6862269.1"
                     /translation="MAERPELPEGRELPEARPAAHRLRWGFLVWVVPVVALVVGAWLG
                     IESLLNRGPTISVTFKTASGLEPGKTKVKYKDVDIGTVRSIGLSDDHRDVVVTAELTP
                     QARGLIVEDTRFWVVRPRITGGQAFGLGTLLSGAYIALDPGKSKAGRREFTGLENPPV
                     VLSDVPGRQFILRAEDLGSIDIGSPVYYRHVRVGQVVSAELDAKGQGVTFVVFVNAPY
                     DRYVTSGTRFWNASGIDASFGAGGFKLETESLASVVIGGVAFQVPPDSDPGQVAPPEA
                     VFVLHKDRDTAFKQAVTIRDVYVLRFRQSVHGLVGGAPVDFRGVPVGEVASVGLEWDA
                     RHREIRPVVEINVYPQRITARLRKRAATVENDVVRARVLQRMIDHGLRAQLKTGNLIT
                     GQLYVSLDFFPNEPKARVDLAQAPLEIPTASGGFTELQSSLENVVKKIEKLPLDEMGA
                     DLKNITASLDVTLRNLNALVERVDSEIAPELKQTLESARGALGEAQGALSQDAPLQGD
                     LHGTLRDVGRAAEAIRNLADYLERHPESLLRGRREEETR"
     gene            complement(2118..2732)
                     /locus_tag="VI319_00070"
     CDS             complement(2118..2732)
                     /locus_tag="VI319_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY6862270.1"
                     /translation="MAAAMGWVACRVCGQLNRGRADARACCARCGSELRLRKPEAMAR
                     TIAFLAAAYVLYLPANLLPILETGELGGAKAADTILSGALKLWHGGWWPLAIVILIAS
                     IVIPFAKLVVLTWLVVSVRRGTATRLRGRTRLYRLVDTIGKWSMVDIYVGALLVGLVQ
                     FEPLATVSPGPAAIAFGAGVVLTMLASHSFDPRLLWDAAGARRG"
     gene            complement(2738..3397)
                     /locus_tag="VI319_00075"
     CDS             complement(2738..3397)
                     /locus_tag="VI319_00075"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY6862271.1"
                     /translation="MATLARDFGSGERAAPALLACPECDLAQRVPAGGAWGAIACCRC
                     GATLCRRIPHALDVTLALMITAAVVFAIANVFPVMSLDMQGRHNEATLLGMAKALHRA
                     GMTPVAVLVALTLIVMPALEIAAMLWLLVPLRLGMAPEGLGLVSRTLAAVRPWAMVEV
                     FTLAALVSIGRLGNLADLHLGVAFWAMGALMFLFALADTIYDEHALWERVAALRPGAA
                     P"
     gene            3502..4761
                     /locus_tag="VI319_00080"
     CDS             3502..4761
                     /locus_tag="VI319_00080"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6862272.1"
                     /translation="MQDRALQQAAPQSFAALRHPGYRAYFAGSALAMMADSVEHVISY
                     WIMFQKFHSPALGGFAMLSHWLPFLFFSVYSGALADRFDPRRLIQIGMLLFMTASLGW
                     GLLFLTDSLQMWHAAVLLVIHGFAGVFWHPAAQLLIHDIVGTAQLQSAVRLGATARYL
                     GLLFGPAVGGAFLLVLGPAAGLLVNMTIYLPLLVWLWKAPYGPRFRKEPSPPRRSIRG
                     LADIASTARAIAGNRAIVSMVMLAGLTSLFVGNGYQPQMPEFAEDLGHGDPDLSYSML
                     LAADAAGALTAGLVLESRGLLQARPRTAIVLAVLWCLGITAFAVSTSYALALAILFVV
                     GFLELSFNAMAQTLVQIHAPAAIRGRVIGLYILFSLGMRAFSGITIGVAGNAIGIHRS
                     LAFSAMALLAVILGIAAFALRTRQARP"
     gene            4758..>5959
                     /locus_tag="VI319_00085"
     CDS             4758..>5959
                     /locus_tag="VI319_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY6862273.1"
                     /translation="MNRDNHDELEAPARGLALRTQLLVPVIVASLPLAVFAAAMMFTL
                     WQFQQNRLETQQQETAAALASVVEKELSSTMRQLQYLGTGPELVRGDFEGFLKRTRQV
                     LAIAKEWTNVVLFSREGQQLVNANFPLPSGKSTATQRHVDQVFATGKPTVSDVFLGPA
                     SGRWIVAVSVPVALQGRTIYVLSATLNLAVFDDLLKERTAAGGVAGIADRQLRLIARA
                     PSPEQYRGKPPVPALLEAMRAAPEGLARFRSYEGIDVFTAWTRMPSVGWTVAFSVPSE
                     RTDVALRRHILLLGSAEILIFLIAAFAAIFIGRRVSGAIERAARQAPGVAEGWRPELP
                     PTGIEELDRLGRALEDAASKLSAEARQRALAERERDQLLALEKEARSAAEAANRSKDE
                     FLAMLGHEL"
BASE COUNT          810 a         2213 c         2095 g          841 t
ORIGIN      
        1 ccttcgcggc gctcgccctg cgccggacgg acgctccaca gcgcctcgac cgcgacgccg
       61 cgcccggcga ccgcctcgaa gcgctgcacg tcgacgaaca cgcgcacctc gggcgatgtg
      121 ccgagcggct gcccggcgac cacggcccat ccgggcccca gccgcgcggc gatgtcggcg
      181 gccagcgtgc gcgcgatccc gaacctgagc ggctcggccc attgatgcag gtcggagatc
      241 tcgacgcggt tcggcgcggt gcgcacgacg agcgcgcggc ggtcgaccat ctcggggatc
      301 gccacctgcc agaccgacgc ggtgcgcacg ttcgtgcccg ccggcgctcc cgcggccggc
      361 gccgcctcgg ggctcagcgt gtaatagctc gtcgaggccg cgcccgcggc gcagccgccg
      421 agcagcacgc agccggcgag cgccaggccc gcggcgaatg cggcggctgc gcgcgtgcgc
      481 gtcatcgcgt ctcctcctcg cgccgtccgc gcagcagcga ctcgggatga cgctcgagat
      541 agtcggcgag gttgcggatc gcctcggccg cgcggccgac gtcgcgcagc gtgccgtgca
      601 ggtcgccctg cagcggcgcg tcctgcgaca gcgcgccctg cgcctcgccg agcgcgccgc
      661 gcgccgactc gagcgtctgc ttcagctcgg gagcgatctc ggaatcgacg cgctcgacca
      721 gggcgttcag gttgcgcagc gtgacgtcga gggacgcggt gatgttcttc aggtccgcgc
      781 ccatctcgtc gagcggcagc ttctcgatct tcttcacgac gttctcgagc gaggactgca
      841 gctcggtgaa gccgcccgac gccgtcggga tctcgagcgg cgcctgcgcg agatcgacgc
      901 gcgccttcgg ctcgttcggg aagaagtcga ggctcacgta gagctggccg gtgatcaggt
      961 tgccggtctt cagctgcgcg cgcaggccgt gatcgatcat gcgctgcagg acccgggccc
     1021 ttaccacgtc gttctcgacc gtcgcggcgc gcttgcgcag gcgcgcggtg atgcgctgcg
     1081 gatagacgtt gatctcgacg acgggccgga tctcgcggtg ccgggcgtcc cattcgagcc
     1141 cgacgctcgc cacctcgccc accggcacgc cgcggaagtc gaccggcgcg ccgccgacga
     1201 gcccgtgcac cgactgccgg aagcgcagca cgtagacgtc gcggatggtg accgcctgct
     1261 tgaacgcggt gtcgcggtcc ttgtgcagca cgaacaccgc ctcgggcggc gccacctgcc
     1321 cgggatcgga gtccggcggc acctggaacg ccacgccgcc gatgacgacc gatgcgagcg
     1381 actcggtctc gagcttgaag ccgcccgcgc cgaacgacgc gtcgatgccg ctcgcgttcc
     1441 agaagcgcgt tccgctcgtc acgtagcggt cgtacggcgc gttgacgaag acgacgaagg
     1501 tgacgccctg ccccttggcg tcgagctccg cgctcaccac ctgcccgacg cgcacgtggc
     1561 ggtagtacac gggcgagccg atgtcgatcg agccgaggtc ctcggcgcgc aggatgaact
     1621 ggcgacccgg gacgtcggag agcacgaccg gcgggttctc gagccccgtg aactcgcggc
     1681 gccccgcctt cgacttgccc gggtcgagcg cgatgtacgc gcccgagagc agcgtgccga
     1741 gcccgaacgc ctgtccgccg gtgatgcgcg gccgcaccac ccagaagcgc gtgtcctcga
     1801 cgatcagccc gcgcgcctgc ggcgtgagct cggcggtgac gacgacgtcg cggtgatcgt
     1861 ccgacaagcc gatcgagcgc accgtgccga tgtcgacgtc cttgtacttg accttcgtct
     1921 tcccgggctc gaggccgctc gcggtcttga acgtgacgct gatggtcggc ccgcgattga
     1981 gcaggctctc gatcccgagc caggcgccga cgacgagcgc gacgaccggg accacccaca
     2041 cgaggaagcc ccagcgcagc cggtgcgccg ccggccgcgc ctcgggcagc tcgcgcccct
     2101 cggggagctc gggccgctca gccacggcgc gctcccgcgg catcccacag cagccgcgga
     2161 tcgaagctgt gcgacgcgag catggtcagc acgacacccg cgccgaaggc gatcgccgcc
     2221 ggccccggcg acaccgtcgc gagcggctcg aactgcacca ggccgacgag cagcgcgccg
     2281 acgtagatgt cgaccatcga ccacttgccg atcgtgtcga ccagccggta gagccgcgtg
     2341 cgcccgcgca gccgcgtcgc ggtcccgcgg cgcacgctga ccaccagcca cgtgagcacg
     2401 accagcttcg cgaacgggat gacgatgctc gcgatcagga tcacgatcgc gagcggccac
     2461 cagccgccgt gccagagctt cagcgcgccg ctcaggatgg tgtccgccgc cttcgcgccg
     2521 ccgagctcgc cggtctcgag gatcggcagc aggttcgccg gcaggtagag cacgtaggcc
     2581 gcggcgagga aggcgatcgt gcgcgccatc gcctcgggct tgcgcagccg cagctcgctg
     2641 ccgcagcgcg cgcagcacgc ccttgcatcg gcgcgcccgc ggttcagctg cccgcagacg
     2701 cggcacgcga cccagcccat cgccgccgcc atggcgctca aggcgcagcc cccgggcgca
     2761 gcgcggcgac gcgctcccag agcgcgtgct cgtcgtagat cgtgtccgcc agcgcgaaca
     2821 ggaacatcag cgcccccatc gcccagaacg cgacgccgag atgcaggtcg gcgaggttgc
     2881 cgagccggcc gatcgacacc agcgcggcga gcgtgaagac ctcgaccatc gcccagggcc
     2941 gcaccgcggc gagcgtcctc gacaccaggc ccaggccctc gggcgccatg ccgagcctga
     3001 gcggcacgag cagccacagc atggcggcga tctccagcgc cggcatcacg atcagcgtca
     3061 gcgcgacgag gaccgccacc ggcgtcatgc ccgcgcggtg cagggccttc gccatcccga
     3121 gcagggtcgc ctcgttgtgc cgcccctgca tgtcgagcga catgaccggg aagacgttgg
     3181 cgatcgcgaa cacgaccgcg gcggtgatca tgagggcgag cgtgacgtcg agggcgtgcg
     3241 ggatgcggcg acacagcgtc gcgccgcagc gacagcaggc gatggccccc cacgcgccgc
     3301 ccgccgggac gcgctgcgcg aggtcgcact ccgggcaggc gagcagcgcg ggagctgcgc
     3361 gctcgcccga gccgaagtcg cgtgcgaggg tggccacggt gggatcttat tgtcggggcg
     3421 tcagttggag gctccggcgg gcccaaaggt tcattgcgcg ccggggcgcc gggtgctcat
     3481 ccggtataaa gtgtgtgccc catgcaggat cgggccttac agcaggcggc gccccagtcc
     3541 ttcgccgccc tgcgccaccc cggctaccgc gcctatttcg ccggctccgc gctggcgatg
     3601 atggccgact cggtcgagca cgtgatcagc tactggatca tgttccagaa gttccactcg
     3661 ccggcgctcg gcggcttcgc gatgctgtcg cactggctgc cgttcctgtt cttctcggtg
     3721 tattcgggag cgctcgccga ccgcttcgac ccgcggcggc tgatccagat cggcatgctg
     3781 ctgttcatga ccgcgtcgct cggctgggga ctgctgttcc tgaccgactc cctgcagatg
     3841 tggcacgccg cggtgctgct cgtgatccac ggcttcgccg gcgtgttctg gcatcccgcg
     3901 gcgcagctcc tgatccacga catcgtcggc acggcgcagc tgcagagcgc ggtgcggctc
     3961 ggcgccacgg cgcgctacct cgggctgctc ttcgggccgg cggtgggcgg ggcgttcctg
     4021 ctcgtcctcg gcccggcggc ggggctcctc gtcaacatga cgatctacct cccgctgctc
     4081 gtgtggctgt ggaaggcgcc ctacgggccg cgattccgca aggagccgtc gccgccgcgg
     4141 cgctcgatcc gcggcctcgc cgacatcgcc tccacggcgc gcgccatcgc cggcaaccgc
     4201 gccatcgtct cgatggtgat gctcgccggg ctgacgtcgc tcttcgtcgg caacggctac
     4261 cagccgcaga tgcccgagtt cgccgaggac ctcggccacg gcgaccccga cctctcgtac
     4321 agcatgctgc tcgccgccga cgcggccggc gcgctgaccg cgggcctcgt gctcgagagc
     4381 cgcgggctgc tgcaggcgcg tccgcgcacc gcgatcgtgc tcgccgtcct gtggtgcctg
     4441 gggatcacgg ccttcgccgt ctcgacctcg tacgcgctcg cgctggcgat cctgttcgtc
     4501 gtcggcttcc tcgagctgtc cttcaacgcg atggcgcaga cgctggtgca gatccacgcg
     4561 ccggccgcga tccgcggccg ggtgatcggc ctctacatcc tgttcagcct cggcatgcgc
     4621 gccttctcgg gcatcaccat cggcgtcgcg ggcaacgcca tcggcatcca ccgctcgctc
     4681 gccttctcgg cgatggcgct gctcgcggtg atcctcggca tcgcggcctt cgcgctgcgc
     4741 acgcggcagg cgcgcccgtg aatcgggaca atcacgacga gctcgaagcg ccggcgcgag
     4801 gcctggcgct gcgcacgcag ctgctcgtgc cggtgatcgt cgcgtcgctg ccgctcgcgg
     4861 tgttcgccgc ggcgatgatg ttcacgctgt ggcaattcca gcagaaccgc ctcgagacgc
     4921 agcagcagga gaccgcggcc gcgctcgcga gcgtggtcga gaaggagctg tcgagcacga
     4981 tgcgccagct ccagtacctc ggcaccggcc ccgagctggt gcgcggcgac ttcgagggct
     5041 tcctgaagcg cacgcgccag gtgctggcga tcgcgaagga gtggaccaac gtcgtgctgt
     5101 tctcgcgcga ggggcagcag ctcgtcaacg ccaacttccc gctgccctcg ggcaagagca
     5161 ccgcgacgca gcgccacgtc gaccaggtct tcgcgaccgg aaagccgacg gtgtcggacg
     5221 tgtttctcgg gcccgcgagc ggccgctgga tcgtcgcggt gagcgtcccg gtcgcgctgc
     5281 aggggcgcac gatctacgtg ctgtcggcga cgctgaacct cgccgtcttc gacgatctgc
     5341 tgaaggagcg cacggccgcc ggcggcgtcg cgggcatcgc cgaccggcag ctgcgcctca
     5401 tcgcacgcgc gccctcgccc gagcagtacc gcggcaagcc gccggtgccg gcgctgctcg
     5461 aggcgatgcg cgctgcgccc gagggtctcg cgcgcttccg cagctacgag ggcatcgacg
     5521 tgttcacggc ctggacgcgc atgccctcgg tcggctggac tgtcgccttc tcggtgccca
     5581 gcgagaggac cgacgtggcg ctgcgccggc acatcctgct gctcggcagc gccgagatcc
     5641 tgatcttcct catcgccgcc ttcgccgcga tcttcatcgg ccggcgcgtg tcgggcgcga
     5701 tcgagcgcgc cgcccggcag gcgcccgggg tcgccgaggg ctggcggccg gagctgccgc
     5761 ccacgggcat cgaggagctc gaccgcctgg gacgggcgct cgaggacgcg gcgtccaagc
     5821 tctcggcgga ggcgcgccag cgcgcgctcg ccgagcgcga gcgcgaccag ctgctcgcgc
     5881 tcgagaagga ggcgcgctcg gccgccgagg cggcgaaccg ctccaaggac gagttcctcg
     5941 cgatgctcgg gcacgagct
//
LOCUS       DATDMW010000004         3415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2366287, whole genome shotgun sequence.
ACCESSION   DATDMW010000004 DATDMW010000000
VERSION     DATDMW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366287"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VI319_00090"
     CDS             complement(<1..489)
                     /locus_tag="VI319_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEY6862274.1"
                     /translation="MPSIDDFDYALPPELVAQHPPASRRESRLLRVAADALEDRRFAE
                     LPALLAPGDLLVVNDTRVIKARLLGEKASGGRVEVLVERVVSEREAIAQVRASKTPRP
                     GTTLRLAGALEAEVLGREGEFFRLRFGASVLNALERHGSVPLPPYIERAADDADAGRY
                     QTV"
     gene            521..607
                     /locus_tag="VI319_00095"
     tRNA            521..607
                     /locus_tag="VI319_00095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:555..557,aa:Leu,seq:caa)
     gene            695..946
                     /locus_tag="VI319_00100"
     CDS             695..946
                     /locus_tag="VI319_00100"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY6862275.1"
                     /translation="MLTDWPYPWHMMGWGWGFGWIFPLLMISGIVFCLVMMSRMFGHA
                     HGHRDSSSSALELLNERFARGEISKEEFEEKRAVLARRP"
     gene            943..1149
                     /locus_tag="VI319_00105"
     CDS             943..1149
                     /locus_tag="VI319_00105"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY6862276.1"
                     /translation="MSAPVYVCPMHAEVRAAQPGTCPHCHMKLVSEGTRFAILRHMTG
                     SPLPIVVMVAVMLALMAAAMMLLK"
     gene            1221..1643
                     /locus_tag="VI319_00110"
     CDS             1221..1643
                     /locus_tag="VI319_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6862277.1"
                     /translation="MKILLATDGSNHARKAVDYVIKHSAMLGDEPDLTLVHVQARLPN
                     RAAAALSRTAVERYYRDQTEKALAPARRALSAKGIPFKEAKLFGDAGETIASFATRGK
                     FSLVVMGSRGMGGFGSIVLGSVAHRVLAGCKVPALIIR"
     gene            1663..2646
                     /locus_tag="VI319_00115"
     CDS             1663..2646
                     /locus_tag="VI319_00115"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY6862278.1"
                     /translation="MKVCVVGAGAIGGWTAGWLARAGHDVRLVARGAHLEALRRDGLT
                     LESGGERRTFRLPASAEPGDFGPQDAVFLCLKTYAIAPMLPRLAPLLGPATAVVPAIN
                     GLPWWYFHAEGGARDGQPIECLDPGGRMLAALDSRRVVGCVVHGAAEVVSPGVVRHTA
                     GNRFILGEPDGGRSARVLALAAAMTAAGFDAPVTPRIRVEIWTKLIGNLAYNPVAALT
                     LAWMQEINANPDLLALIRVLMQEAMRVAEAYGVTIPMTLDERIEVARKIGRARISMHQ
                     DVERGRPLETEAIIGSVAELARRAAVATPMIDAMLALLVERSRHRGPGALQ"
     gene            2677..3225
                     /locus_tag="VI319_00120"
     CDS             2677..3225
                     /locus_tag="VI319_00120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEY6862279.1"
                     /translation="MKKALLIVDIQNDYFPGGRMELEGSQSASLRAGALLASFRERRM
                     PIFHVQHVSVRPGAAFFLPGTDGVKIHPNVAPSGSEPVVQKHFPNAFRETALLDSLRQ
                     GGIQELVIAGMMTHLCIDASTRAAADLGFKCFLAHDACATRALSFGDTRVPAEQVHCS
                     FLSALSGSYATVQTAAELNKSL"
     gene            complement(3222..>3415)
                     /locus_tag="VI319_00125"
     CDS             complement(3222..>3415)
                     /locus_tag="VI319_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY6862280.1"
                     /translation="SRRGAPSPLARRAGDALLGLFILQIALGISTLLLRVPVPLAAAH
                     QAGALAVFTAALVAAHALR"
BASE COUNT          485 a         1244 c         1192 g          494 t
ORIGIN      
        1 gaccgtctgg tagcggccgg cgtcggcgtc gtccgcggcg cgctcgatgt agggcggcag
       61 cggcaccgag ccgtgtcgct cgagcgcgtt cagcacgctc gcgccgaagc gcaggcggaa
      121 gaactcgcct tcgcgaccga gcacctcggc ctcgagggcg ccggcgagcc gcagcgtcgt
      181 cccggggcgc ggcgtcttgc tcgcgcgcac ctgcgcgatc gcctcgcgct ccgagacgac
      241 gcgctcgacc agcacctcga cgcgcccgcc gctggccttc tccccgagca ggcgcgcctt
      301 gatcacgcgg gtgtcgttca cgacgagcag gtccccgggc gcgagcagcg cgggaagctc
      361 ggcgaatcgc cggtcctcga gcgcgtcggc cgcgacgcgc agcaggcggc tctcgcggcg
      421 cgacgccggc ggatgctgcg cgaccagctc gggcggcagc gcatagtcga agtcgtcgat
      481 ggaaggcacg gtgctccgag tataatttcg accctccgcg gccgggatgg cggaactggt
      541 agacgcagcg gactcaaaat ccgccggtgg cgacaccatg agggttcgat tccctctccc
      601 ggcaccagcg agatcgacgc ttgattcagg tcaagacaac ccgacgggag gctcctagga
      661 tgaatttgta cttcctttcc ggaaggagca cgccatgctg accgattggc cttatccctg
      721 gcacatgatg ggctggggct ggggattcgg ctggatcttc ccgctcctga tgatctccgg
      781 gatcgtgttc tgcctcgtga tgatgagccg catgttcggc catgcccacg gccatcgtga
      841 cagctcgtct tccgcactcg agctcctgaa cgagcgcttc gcccggggcg agatctcgaa
      901 agaggagttc gaggagaagc gcgccgtgct cgcgcgccgg ccgtgagcgc tccggtctac
      961 gtctgcccga tgcacgcgga ggtccgggcc gcgcagcccg gcacctgccc gcattgccac
     1021 atgaagctgg tgagcgaggg cacgcgcttc gcgatcctca ggcacatgac cggcagtccc
     1081 ctgcccatcg tggtgatggt cgcggtgatg ctggccctga tggccgcggc gatgatgctg
     1141 ctgaagtagc cggttcgcgc tccgggcccc gggatggcac aatgcggccc ttccgcaaca
     1201 aaaaccaccc gggagtccca gtgaagatcc tcctcgccac cgacggctcg aaccatgcgc
     1261 gcaaggcggt cgattacgtc atcaagcact ccgccatgct cggcgacgag cccgacctca
     1321 cgctggtgca cgtccaggcg cgcctgccga accgcgccgc tgcggcgctc agccgcacgg
     1381 ccgtggaacg ctactaccgc gaccagaccg agaaggcgct cgcgccggcg cggcgcgcgc
     1441 tgtccgcgaa gggcatcccg ttcaaggagg cgaagctctt cggcgacgcg ggcgagacga
     1501 tcgcctcgtt cgccacgcgg gggaagttct cgctcgtcgt catgggctcg cgcggcatgg
     1561 gcggcttcgg cagcatcgtg ctcggctcgg tcgcgcacag ggtgctcgcc ggctgcaagg
     1621 tgcccgcgct gatcatccgc tagcccgccc gccgggtcgg ccgtgaaggt ctgcgtggtc
     1681 ggcgccggcg ccatcggcgg ctggaccgcc ggctggctcg cgcgcgccgg ccacgacgtg
     1741 cgactcgtcg cgcgcggcgc gcatctggag gctttgcggc gcgacggcct cactctcgaa
     1801 tcgggcggcg agcgccgcac gttccggctc ccggcgtccg ccgagcccgg ggacttcggg
     1861 ccgcaggacg cggtctttct ctgcctcaag acctacgcca tcgcgccgat gctgccgcgc
     1921 ctcgcgccgc tcctcggccc ggccaccgcg gtggtgcccg cgatcaacgg cctgccctgg
     1981 tggtacttcc acgccgaagg cggcgcccgg gacgggcagc cgatcgagtg cctcgacccg
     2041 ggcggccgca tgctcgccgc cctggattcc cgccgcgtcg tgggctgcgt cgtgcatggc
     2101 gcagcggagg tggtctcgcc gggcgtggtc cggcacaccg cgggaaaccg cttcatcctg
     2161 ggcgagcccg acggcggccg ctccgcgcgc gtgctcgcgc tcgccgcggc gatgaccgcc
     2221 gccggcttcg acgcgcccgt caccccgcgc atccgggtcg agatctggac caagctgatc
     2281 ggcaacctcg cgtacaaccc ggtcgcggcg ctgacgctcg cctggatgca ggagatcaac
     2341 gccaatcccg acctgctcgc cctgatccgc gtcctgatgc aggaggcgat gcgcgtcgcc
     2401 gaggcctacg gcgtgaccat cccgatgacg ctcgacgagc gcatcgaggt cgcgcgcaag
     2461 atcggccgcg cgaggatctc gatgcaccag gacgtcgagc gcggccgccc gctcgagacc
     2521 gaggcgatca tcggctcggt ggccgagctc gcgcgccggg ccgcggtcgc gacgcccatg
     2581 atcgacgcga tgctggcgct gctcgtcgag cgctcgcgcc accgcggccc gggcgcgcta
     2641 caatgagccg gcagcccagg gaagggggac gcggggatga agaaggcttt gctgatcgtc
     2701 gatatccaga acgactactt tcccggcggg cgcatggagc tcgaaggcag ccaatcggcg
     2761 agcctgcgcg ccggcgcgct gctcgcgtcc ttccgcgagc gccgcatgcc gatcttccac
     2821 gtgcagcacg tctcggtgcg cccgggcgcc gcgttcttcc tgcccgggac cgacggcgtg
     2881 aagatccatc ccaacgtcgc gccctcgggc agcgagcccg tggtgcagaa gcacttcccc
     2941 aacgctttcc gcgagaccgc gctgctcgac tcgctgcgcc agggcggcat ccaggagctc
     3001 gtgatcgccg ggatgatgac ccacctgtgc atcgacgcgt ccacgcgcgc cgcggccgac
     3061 ctcggcttca agtgcttcct cgcgcacgac gcgtgcgcga cgcgtgcgct cagcttcggc
     3121 gacacgcgcg ttcccgcgga gcaggtgcac tgctcgttcc tctcggcgct gtccggatcc
     3181 tacgcgacgg tgcagaccgc agccgagctg aacaagtccc tctagcgcag cgcgtgcgcg
     3241 gcgaccagcg ccgcggtgaa cacggcgagc gcgcccgcct ggtgggccgc tgcgagcggc
     3301 accgggaccc gcagcagaag cgtcgagatc ccgagcgcga tctgcaggat gaacagcccg
     3361 agcagcgcgt cgcccgcgcg ccgcgcgagc ggcgagggcg cgccgcgccg tgagc
//
LOCUS       DATDMW010000005        10591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421 k141_1145,
            whole genome shotgun sequence.
ACCESSION   DATDMW010000005 DATDMW010000000
VERSION     DATDMW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VI319_00130"
     CDS             <1..201
                     /locus_tag="VI319_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862281.1"
                     /translation="CNGLLYLLSRDRETEPWQHSGASVREDREGDHIRCPVCAYRVAV
                     RRSDATPGWGYEIDLPPRAGGG"
     gene            complement(212..352)
                     /locus_tag="VI319_00135"
     CDS             complement(212..352)
                     /locus_tag="VI319_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862282.1"
                     /translation="MELTLRNGARFTADFFHCTECSLHFVHPDRFMRAIDAAAGEAAA
                     LV"
     gene            716..886
                     /locus_tag="VI319_00140"
     CDS             716..886
                     /locus_tag="VI319_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862283.1"
                     /translation="MIYTLERHDGEAEWVHRGSPLEYDERGPFVRCPGCRTPVRHRRC
                     GNVPGFDHEVSQ"
     gene            complement(893..1915)
                     /locus_tag="VI319_00145"
     CDS             complement(893..1915)
                     /locus_tag="VI319_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6862284.1"
                     /translation="MTRAAVLLVALVAGPAAAQSFAQLAADASPARNERLVAGAKKEG
                     SLTLYTSNAAQTIKLLAADFEKRYGVKVEVWRASSAKVLQRLVAEKQAGRWDFDAASA
                     SSPELEALYREGLLQEVNSRWHAGMLEGTVPAHRGWAPQFLNVFVQAYNTKAVKKDEL
                     PKRWADLLDPKWKGRLGVEAKAGEWYCALAKTAGGGDAVGTLSRVGGRNGWSVRNGNS
                     LLVNMVVSGEVPLALAVYSHMIDEAKAQGAPVDWFSIGPMIGRSNGIGVSRRPRHPDA
                     ALLFYEYSLGEGQQILAKLKYVSPLASMPSPFRGAGIAFVDPSASPAEVERCDDAYEA
                     LIKLGR"
     gene            complement(1912..2985)
                     /locus_tag="VI319_00150"
     CDS             complement(1912..2985)
                     /locus_tag="VI319_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6862285.1"
                     /translation="MDQGIDGVIVSADSHVMEPVDLWKKGVPGNYRDAAPLYPPHKLG
                     EGFQQREGGWDPNARIAEMEIDGLSAEVLYPTLLLKQFALEDAGLQQACFRVYNDWLI
                     DYCSVNRERLVGVAAISIYDVEHAVAELGRCRKAGLKGALIWQAPHPDLPFHSKHYDR
                     FWAAAQDLDAPVSMHILTGHSYHAKERKGIEHYRGSVNLKLMDVANALFEFIFYGVLE
                     RFPRLRLVTVENESGWLPFMVQQWDYYWRRFKGVNPPPITKAPGEYVREQIYSCFFND
                     SVCGRQLEWWGQDNLMWSNDFPHPNSTWPNSLKVIRRDLGHLPVETQTKVLAANACRL
                     YGLDRAKLPSGVSKAAQAAQAAA"
     gene            complement(2995..3489)
                     /locus_tag="VI319_00155"
     CDS             complement(2995..3489)
                     /locus_tag="VI319_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6862286.1"
                     /translation="MSTQAIDPKDVKDITRSGPKADAKKPAFYKLRAQMLEQGRTNTP
                     LARTDNMWATLKVYASGGENGLHTHPNEDHTFVVLQGSARYYDADGGHTDVKRHEGIM
                     LPAGAYYWFEATSKEPLVLLRVGCRTGSDTGARLNIRGDAMPGDSKENKRVPVVYRDG
                     EFFE"
     gene            3563..4573
                     /locus_tag="VI319_00160"
     CDS             3563..4573
                     /locus_tag="VI319_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6862287.1"
                     /translation="MRRPALLAALLAALLAIELSCSAQENAPLVAAAQKEGSVTLYTS
                     FAERDLPPIAAAFEKKYGVKLRVWRSASEKVLQRTLAEAAARRYDVDAVHSSAMEMEA
                     LHREGLLRAVDSPYAGDLIEGAVRPHREWTASYLSIWVQAYNTRLVKKDELPRRLEDL
                     LDPRWKGRLGIESHVWDWYSSVILRMGEQKGLAFFRELVAKNGLSVRSGHTLLNNLVV
                     AGDVPMAITMYNFIADGAKRKGADVDWIALEPLIGRMSGIGVMRRAPHPNATLLFHDW
                     LLSPEGQQVLASLDYVPTNAKVASPFAKRKFTLVDPAFALDHSDRWEKSFEDVIVKGA
                     RR"
     gene            4570..5598
                     /locus_tag="VI319_00165"
     CDS             4570..5598
                     /locus_tag="VI319_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6862288.1"
                     /translation="MTRICVALALVAALSAARAQTLQELATYAGPDREQRLVEGAKRE
                     GALVFYTTFPPQYADQLLDPFRARTGIKVTVWRARSELVLSRVLAEAHAGSPGADVIT
                     VIATATEAMRRENLLQEVRSPHQAELHSAAVPVHRQWVAQLDHVFVHAYNTDKVKKAE
                     LPRAWRDLLDPRWKGRLAIEGDDHEWLSSVIADMGEAQGAAFFRELVAKNGLSVRSGH
                     PLLTNLVASGEIPLALTVYEYSVEQAKKKGSPIDWFVIGSAVSIPNAIAIPKKAQHPH
                     AALLLYDWLLSAEGQGILAKIGYVPTSTKVESPVKGVTLKYLDSAKLVDEQEKSTQRF
                     QQLILQGR"
     gene            complement(5600..6769)
                     /locus_tag="VI319_00170"
     CDS             complement(5600..6769)
                     /locus_tag="VI319_00170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY6862289.1"
                     /translation="MRIYQFSEQPYPDAWENPSIRVNLPSRNMDPEVMAQNYHDRLDE
                     WLLADELGLDIMINEHHATATCCCPVAAVPLAILARQTKRARLLTLGYPIANRLDPVR
                     IAEEFAMIDVISRGRLEMGLVRGVPYEVPLAVRSAVGQMDRFWEAHDLILKAMTSTDG
                     PFDWQGEHFQYRNVNVWPRVYQQPHPPVWITGRAPQNIREIAERGYVLGTFLSGFQTR
                     QLFDHYRKACADLGRPAPAEDRFSYLGIVAIAQDRKEAMRRAEVMVGYLRSSGRVAEP
                     FGTPPGYFSVGDAMRMIKARGARGTTTRDGKFVDTHHGAIEDLVKAGIMFVGTPDDVY
                     GQLAEFIDNIGGLGHFLMMGHAGHLTHAEAMEQITLVAKEVLPRLNARRPAAKAA"
     gene            complement(6766..7563)
                     /locus_tag="VI319_00175"
     CDS             complement(6766..7563)
                     /locus_tag="VI319_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6862290.1"
                     /translation="MEGASLLKIGGLDIEIVRRGRGRPVLFLHPHIGFWGAERFLDAM
                     AKHAEVIAPAHPGFGRSALAKGMTTVDDLSYFYLDLLEQLDLRDVALVGASFGGWLAA
                     AIATKSCERLARLAMLGAVGVKLGARDKTDVVDIFATPRSKWEEVSFADPAHGRREYS
                     ALPDEELQAMARNREATALFAWNPYMYDPKLLGRLHRIRIPTLFLWGAADRFAPAGYG
                     RGYCAAVPGAVFEEIAGAGHFPHIEQPEAVAARIAEFALGAQLEKQP"
     gene            complement(7565..8491)
                     /locus_tag="VI319_00180"
     CDS             complement(7565..8491)
                     /locus_tag="VI319_00180"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862291.1"
                     /translation="MVHGSALAQTFPSHPIKIVVAFPVGGGSDIAARLVGQRLAERFG
                     QPVVIENRVGANGNIGGEAVARSAPDGYTLVMGSNANITTNPHLMKLDWDPMKDLAPV
                     AMLTVNPLLLFVNPDVVPVKTFAEFLDYVKARDGKVYYASAGNGSPAHLAGELLKIVA
                     RVGMVHVPYKGGPPGVNDVLGGRVGVMLAAAPTVMPHIRSGKLRGLATTGARRSSATP
                     DIPTVAESGFPGFDVVIWNAVFAPGATPRPVLAKLHDGIGAVLEEPDTRALLEQQGAD
                     PAPMSDAAFRAMLATEYERWGKVIREAHIRVD"
     gene            complement(8597..9661)
                     /locus_tag="VI319_00185"
     CDS             complement(8597..9661)
                     /locus_tag="VI319_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY6862292.1"
                     /translation="MAASTETRLRRYDELSLSAQTAYAELFESARAQELHRSIANLSG
                     SFASKTVKGRLYWYYQYRDVNGTVRQLYVGPDTDEVKALIKRAKENPAKALEPLARSA
                     IALGCERVLPRHFRVIRRLSEYGFFRAGGILIGTHAFLAIGNVLGIRWHDGSRTQDVD
                     FAHAGKNISLALPATIQVDVHKALESLEMGFLPISTFGGKTGATYLNPKAPEFRIDFL
                     TTLQRDGDQAFTPPNLNVALQPLKFMEFLLEHPVQAVVFSEEGAVVVNIPLPVRYALH
                     KLVVSGEREEAFRTKSTKDLLQSAALIAYFKDHRDDELRATWHDLLSRGKGWHGRAKD
                     GWSALAKAAPDLEIEKLLKI"
     gene            complement(9772..>10591)
                     /locus_tag="VI319_00190"
     CDS             complement(9772..>10591)
                     /locus_tag="VI319_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6862293.1"
                     /translation="IEYAVHGGAALKGHLYAPRGGGKVPVVIAVHGGGWRLANLENYA
                     HLGPWLAERGYAVLAVTHRLSSASTRVYPEAVHDVRAAVQYARANAAELGIDPERIAL
                     MGDSSGAHLASLVALAGDHPAFKDGNRADPHGGVSTRVRAAVVVYGVFDLVAQWRHDQ
                     LARPRDPIVDRFLGVSLIDDRRPYFEASPLSYVSARNNATAFLVVWGSDDDVVDAKEQ
                     SGAFLEALKQAGHYVRPVVLAGAPHFWIGDPLDEPGSQPGFLAPRLLRFLQAKL"
BASE COUNT         1732 a         3552 c         3576 g         1731 t
ORIGIN      
        1 tgcaacggcc tgctctatct cctttcgcgc gaccgggaga ccgagccctg gcagcactcg
       61 ggcgcctcgg tgcgcgagga ccgcgaaggc gatcacatcc gctgcccggt atgcgcctac
      121 cgcgtcgcag tcagacgcag cgacgcgact ccggggtggg gttacgagat cgacctgccg
      181 ccgagggcgg gcggaggctg attccgacgg gctagacgag cgcggccgct tctccggcgg
      241 cggcgtcgat cgcccgcatg aaacggtccg ggtgcacgaa gtgcagcgag cactccgtgc
      301 agtggaagaa gtcggcggtg aaccgcgcgc cgttgcgcag cgtcagctcc accgccgcaa
      361 acgtacgcga cccgcaaaga ggacagcgga aaaccgcgcg ttgaagtggc cgggactcca
      421 tgatctttcg aggacgatgc cgatgcgctg gcagggtacc gcgcgaattg gacgggactg
      481 cgaagcacga cgttcccgcg cctgagcgca acggacctcg aaggcgcacg gcgtgtaaca
      541 atccagtcac ctccgtgcat gcatcccatc tatcccggca taacagaggc ccatcccccg
      601 gcgcgcgccc acggagtatc gtcgcggcac cggaaattga tcccggtcca gcttccaggg
      661 agggaagtcg atgggcagtt tcgtccgggg agtcgcttgc ccgcggtgct tcaagatgat
      721 ctacacgctg gagcgccacg acggggaggc cgaatgggtg caccggggat cgccgctcga
      781 gtacgacgag cgcggtcctt tcgtgcgctg ccccggatgc cgcacgccgg tgcgccatcg
      841 tcgatgcggg aacgtgcccg ggttcgacca cgaggtctcg cagtagcagg cctcagcgtc
      901 cgagcttgat cagcgcctcg taggcatcgt cgcagcgctc gacctcggcc gggctcgcgg
      961 aggggtcgac gaaagcgatg cccgcgccgc ggaacggcga cggcatgctc gcgagcggcg
     1021 agacgtactt cagcttcgcc aggatctgct gcccttcgcc gagcgaatac tcgtagaaga
     1081 gcagcgccgc gtccggatgc ctcggccggc gcgagacgcc gatgccgttc gagcggccga
     1141 tcatcggccc gatcgagaac cagtcgacgg gggcgccctg cgccttggcc tcgtcgatca
     1201 tgtggctgta gacggcgagc gcgagcggca cctcgcccga cacgaccatg ttgacgagca
     1261 gcgagttgcc gttgcgcacc gaccagccgt tgcgcccgcc cacccggctc agagtcccga
     1321 ccgcgtcgcc gccgcccgcg gtcttcgcca gcgcgcagta ccattcgccg gccttggcct
     1381 cgacgccgag gcggcctttc cacttcgggt ccaggaggtc ggcccagcgt ttcggcagct
     1441 cgtccttctt caccgccttc gtgttgtacg cctgcacgaa gacgttcagg aactggggcg
     1501 cccagccgcg gtgcgccggc accgtgccct cgagcatccc ggcgtgccag cgcgaattca
     1561 cctcctgcag caggccttcg cggtacagcg cctcgagctc gggcgaggac gcgctcgccg
     1621 cgtcgaagtc ccagcggccc gcctgcttct ccgcgaccag gcgctgcagc accttcgccg
     1681 aggacgcgcg ccagacctcg accttcacgc cgtagcgctt ctcgaaatcc gcggcgagaa
     1741 gcttgatggt ctgcgccgcg ttcgaggtgt acagcgtgag cgagccttcc ttcttcgccc
     1801 cggcgacgag gcgctcgttg cgcgcgggcg acgcgtccgc cgcgagctgc gcgaaggact
     1861 gcgccgcggc cgggcccgcg acgagcgcga cgagaaggac ggccgcgcgg gtcaagccgc
     1921 ggcctgcgcc gcctgcgccg ccttcgagac gccggacggc agcttcgccc ggtcgaggcc
     1981 gtagaggcgg caggcgttgg ccgcgagcac cttggtctgc gtctcgacgg gcagatgccc
     2041 gagatcgcgc cggatcacct tgagcgagtt cggccaggtc gagttcggat gcgggaaatc
     2101 gttcgaccac atgaggttgt cctgtcccca ccactcgagc tgccggccgc acaccgagtc
     2161 gttgaagaag cacgagtaga tctgctcgcg cacgtactcg cccggcgcct tggtgatcgg
     2221 cggcgggttc acgcccttga agcggcgcca gtagtagtcc cactgctgca ccatgaaggg
     2281 cagccagccc gactcgttct ccaccgtcac gagcctcaag cgcgggaagc gctcgagcac
     2341 gccgtagaag atgaactcga acagcgcgtt cgccacgtcc atgagcttga ggttgacgct
     2401 gccgcggtag tgctcgatgc ctttgcgctc cttcgcgtga tagctgtgcc ccgtgaggat
     2461 gtgcatgctc accggcgcgt cgaggtcctg cgcggctgcc cagaatcggt cgtagtgctt
     2521 ggagtggaac ggcaggtcgg gatgcggcgc ctgccagatc agcgcgccct tgaggccggc
     2581 cttgcggcag cgcccgagct cggcgaccgc gtgctcgacg tcgtagatcg agatcgccgc
     2641 cacgccgacc aggcgctcgc ggttcacgct gcagtaatcg atcagccagt cgttgtagac
     2701 gcggaagcac gcctgctgca gcccggcgtc ctcgagggcg aactgcttca gcagcagcgt
     2761 cgggtagagg acctcggccg agagaccgtc gatctccatc tccgcgatgc gcgcgttcgg
     2821 gtcccagccg ccctcgcgct gctggaagcc ttcgccgagc ttgtgcggcg gatagagcgg
     2881 cgcggcgtcg cggtaattcc cgggtacgcc cttcttccac aggtcgaccg gctccatcac
     2941 gtgcgagtcg gccgagacga tcacgccgtc aatgccctgg tccatcgtgg gtccctattc
     3001 gaagaactcg ccgtcgcggt acacgacggg cacgcgcttg ttctccttcg agtccccggg
     3061 catcgcgtcg ccgcggatat tgaggcgcgc gcccgtgtcg gagccggtgc ggcagccgac
     3121 gcgcagcagc accagcggct ccttgctggt cgcctcgaac cagtagtacg cgcccgcggg
     3181 cagcatgatg ccctcgtgcc gcttcacgtc ggtgtggccg ccgtcggcgt cgtagtagcg
     3241 cgcgctgccc tgcagcacga cgaaggtgtg gtcctcgttc ggatgcgtgt gcaggccgtt
     3301 ctcgccgccc gaggcgtaga ccttgagcgt ggcccacatg ttgtcggtgc gcgcgagcgg
     3361 cgtgttggtg cgcccctgct cgagcatctg cgcgcgcagc ttgtagaacg ccggcttctt
     3421 cgcgtcggcc ttggggccgg agcgggtgat gtccttgacg tccttcgggt cgatggcttg
     3481 agtcgacatt cgaggctcct tcgcagggag tggactcgat gttagattag cgcggtctcc
     3541 gactcaagaa ccggtgcaga acatgcgtcg tccggcactg ctcgccgcgc tgctcgccgc
     3601 cctgctcgcg atcgagctgt cctgttccgc gcaggagaac gcaccgctcg ttgcggcggc
     3661 gcaaaaggag ggctcggtca cgctctacac cagcttcgcc gagcgcgacc tgccgccgat
     3721 cgccgcggcg ttcgagaaga aatacggagt caagctgcgg gtctggcgct cggcgtccga
     3781 gaaggtgctg cagcgcacgc tcgccgaggc cgcggcccgg cgctacgacg tcgacgcggt
     3841 gcactcctcg gcgatggaga tggaggcgct gcaccgcgag ggactgctcc gggcggtcga
     3901 ctcgccgtac gcgggcgacc tgatcgaagg cgccgtgcgg ccgcaccgcg aatggaccgc
     3961 gagctacctc tcgatctggg tgcaggccta caacacgcgt ttggtgaaga aggacgagct
     4021 gccgcggcgc ctcgaggacc tgctcgaccc gcggtggaag gggcgcctcg gcatcgagtc
     4081 gcacgtctgg gactggtact cgagcgtaat cctgcggatg ggcgagcaga agggcctcgc
     4141 gttcttccgc gagctggtgg cgaagaacgg cctgtcggtg cgctcgggcc atacgctgct
     4201 caacaacctc gtggtcgcgg gcgacgtccc catggcgatc accatgtaca acttcatcgc
     4261 cgacggcgcg aagcgcaagg gcgccgacgt cgactggatc gcgctcgagc cgctgatcgg
     4321 gcgcatgagc ggaatcggcg tgatgcgccg cgcgccgcac ccgaacgcga cgctgctgtt
     4381 ccacgactgg ctgctctcgc ccgaggggca gcaggtcctc gcgtcgctcg actacgtgcc
     4441 gacgaacgcg aaggtcgcat ccccgttcgc gaagcgcaag ttcacgctgg tcgacccggc
     4501 cttcgcgctc gatcacagcg accggtggga gaagtccttc gaggacgtga tcgtgaaggg
     4561 agcgcggcga tgacgcggat ctgcgttgcc ctcgcgctgg tcgcggccct gagcgccgcg
     4621 cgcgcgcaga cgctgcagga gctggcgacc tacgcgggcc cggaccgcga gcagcgcctg
     4681 gtcgagggtg cgaagcgcga aggcgcgctc gtcttctaca ccaccttccc gccgcaatac
     4741 gccgaccagc tgctcgaccc gttccgcgcg cgcaccggca tcaaggtcac cgtctggcgc
     4801 gcgcgctccg agctcgtcct ctcccgcgtg ctcgccgagg cgcacgccgg ctcgcccggc
     4861 gccgacgtga tcaccgtcat cgccaccgcg accgaggcga tgcggcgcga gaacctgctg
     4921 caggaagtgc gctcgcctca ccaggccgag ctccactccg cggcggtgcc cgtgcaccgg
     4981 caatgggtcg cgcagctcga ccacgtgttc gtccacgcct acaacaccga caaggtgaag
     5041 aaggccgagc tgccgcgcgc ctggcgcgac ctgctcgacc cgagatggaa aggcaggctc
     5101 gcgatcgagg gcgacgacca cgagtggctg tcgtcggtga tcgcggacat gggcgaggcg
     5161 caaggcgccg cgttcttccg cgagctggtc gcgaagaacg ggctctcggt gaggagcggc
     5221 cacccgctgc tcaccaacct cgtcgcctcg ggcgagatcc cgctcgcgct caccgtctac
     5281 gaatacagcg tcgagcaggc gaagaagaaa ggctcgccga tcgactggtt cgtcatcggt
     5341 tcggcagtaa gcattcccaa cgcgatcgcc atcccgaaga aggcgcagca cccgcacgcg
     5401 gcgctgctgc tctacgactg gctgctgagc gcggagggcc aaggcatcct ggcgaagatc
     5461 ggctatgtcc cgaccagcac gaaggtggaa tcgcccgtga aaggcgtgac gctcaagtac
     5521 ctcgattcgg cgaagctggt cgacgagcag gagaaatcga cgcagcggtt ccagcagctg
     5581 atcctgcagg ggcgatgatt caggcggcct tcgccgccgg ccgccgcgcg ttcagccgcg
     5641 gcagcacttc cttcgcgacc agcgtgatct gctccatcgc ctcggcgtgc gtcaggtgcc
     5701 cggcgtggcc catcatcagg aagtggccga ggccgccgat gttgtcgatg aactcggcga
     5761 gctgcccgta gacgtcgtcg ggcgtgccga cgaacatgat gcccgccttg accaggtcct
     5821 cgatcgcgcc gtggtgcgtg tcgacgaact tgccgtcgcg cgtggtcgtg ccgcgcgcgc
     5881 cgcgcgcctt gatcatgcgc atcgcgtcgc cgaccgagaa gtatccgggc ggcgtgccga
     5941 acggctcggc gacgcggccc gaggagcgga gataaccgac catgacctcg gcccggcgca
     6001 tcgcttcctt gcggtcctgc gcgatcgcga cgatgccgag ataggagaag cggtcctcgg
     6061 cgggcgccgg gcggccgagg tcggcgcagg ccttgcggta atgatcgaag agctggcgcg
     6121 tctggaagcc cgagagaaaa gtccctagca cgtagccgcg ctcggcgatc tcgcggatgt
     6181 tctgcggcgc gcgccccgtg atccacaccg gcgggtgcgg ctgctggtac acgcgcggcc
     6241 agacgttgac gttgcggtac tggaagtgct cgccctgcca gtcgaacggc ccgtcggtcg
     6301 aggtcatcgc cttgaggatg aggtcgtgcg cctcccagaa gcggtccatc tggccgacgg
     6361 ccgagcgcac cgcgagcggc acctcgtagg gcacgccgcg gaccagcccc atctcgagcc
     6421 ggccgcgcga gatcacgtcg atcatcgcga actcctcggc gatgcgcacc gggtcgaggc
     6481 ggttggcgat cgggtagccg agcgtgagca ggcgcgcgcg cttcgtctgg cgcgcgagga
     6541 tcgcgagcgg caccgccgcg accgggcagc agcaggtcgc cgtcgcgtgg tgctcgttga
     6601 tcatgatgtc gaggccgagc tcgtcggcga gcagccactc gtcgaggcgg tcgtggtagt
     6661 tctgcgccat gacctccggg tccatgttgc ggctcggcag gttgacccgg atcgacgggt
     6721 tctcccacgc gtcgggatag ggctgctccg agaactggta gatcctcatg gctgcttctc
     6781 cagttgcgca ccgagcgcga actcggcgat ccgggcggct accgcctcgg gctgctcgat
     6841 gtgcgggaag tggccggctc ccgcgatctc ctcgaacacg gcaccgggaa cagccgcgca
     6901 gtagccgcgg ccgtagcccg cgggcgcgaa gcggtcggcc gcgccccaga ggaacagcgt
     6961 cgggatgcgg atgcgatgca gccgcccgag gagcttcggg tcgtacatgt acggattcca
     7021 cgcgaacagc gccgtcgcct cgcggttgcg cgccatcgcc tgcaattcct cgtcgggcag
     7081 cgccgagtac tcgcggcgcc cgtgcgccgg gtcggcgaac gacacttcct cccatttcga
     7141 gcgcggcgtg gcgaagatgt ccaccacgtc ggtcttgtcg cgcgcgccga gcttgacgcc
     7201 gaccgcgccc agcatcgcga ggcgcgcgag ccgctcgcag ctcttggtcg cgatcgcggc
     7261 cgcgagccag ccgccgaagg acgcgccgac cagggcgacg tcgcgcaggt cgagctgctc
     7321 gagcaggtcg aggtagaagt acgacaggtc gtcgaccgtg gtcatgccct tcgcgagcgc
     7381 cgagcgcccg aagcccgggt gcgcgggcgc gatcacctcc gcgtgcttcg ccatcgcgtc
     7441 gaggaagcgc tcggcgcccc agaatccgat gtgcggatgc aggaacagca ccggccggcc
     7501 gcggccgcgc cgcacgatct cgatatccag gccgccgatc ttcaagaggc tcgcgccttc
     7561 catttcaatc caccctgatg tgcgcctcgc ggatcacctt gccccagcgc tcgtactcgg
     7621 tcgcgagcat agcgcggaac gccgcgtcgc tcatcggcgc cggatcggcg ccctgctgct
     7681 cgagcagcgc gcgcgtgtcg ggctcctcga gcaccgcgcc gatcccgtcg tgcagcttcg
     7741 cgagcaccgg gcgcggcgtc gcgcccggcg cgaacaccgc gttccagatc acgacgtcga
     7801 accccgggaa cccggactcg gcgaccgtcg ggatgtcggg cgtcgcgctc gagcgccggg
     7861 cgcccgtcgt cgcgaggccg cgcagcttgc ccgagcggat gtgcggcatc acggtcggcg
     7921 ccgccgcgag catcacgccg acgcggccgc cgagcacgtc gttcacgccc ggcggcccgc
     7981 ccttgtacgg gacgtgaacc atgccgacgc gggcgacgat cttcagcagc tcgcccgcga
     8041 ggtgcgcggg cgagccgttg ccggcggagg cgtagtacac cttgccgtcg cgcgccttca
     8101 cgtagtcgag gaactcggcg aaggtcttca ccggcacgac gtcgggattc acgaacagca
     8161 gcagcgggtt caccgtcagc atcgccaccg gcgcgaggtc cttcatcggg tcccagtcga
     8221 gcttcatcag gtgcgggttg gtggtgatgt tcgcgttgct gcccatcacg agcgtgtagc
     8281 cgtcgggcgc cgagcgcgcc accgcctcgc cgccgatgtt gccgttcgcg ccgacgcggt
     8341 tctcgatcac gaccggctgc ccgaagcgct cggcgaggcg ctggccgacg aggcgcgccg
     8401 cgatgtcgga tcctccgccg accgggaaag cgaccacgat cttgatcggg tgcgagggga
     8461 aggtttgtgc cagggcagat ccgtggacca tcagcaccaa aaacgaaaga gccaggaact
     8521 gacttatcgt tcggctagtt ctgttcggcg tcacatcagt ctcacgatat aagagatatc
     8581 ccgcgacggg gtttcgctag atcttcagga gcttctctat ctcaagatcc ggggcagcct
     8641 tggcgagcgc gctccacccg tccttggctc gtccatgcca gccttttccc cgggacagca
     8701 aatcatgcca ggtggcgcgt aactcatcat ctctatgatc tttgaaataa gctatcaatg
     8761 ccgccgattg caacagatcc ttagtcgact tggtgcggaa cgcctcttcg cgttcacccg
     8821 agacgacgag cttgtgcaaa gcgtagcgta ccggcaacgg tatatttacg accacggcgc
     8881 cttcttcgct gaacaccacc gcttgaaccg gatgctcaag taggaattcc ataaatttga
     8941 gtggctgtag agccacgttc aggtttggag gtgtaaatgc ctggtcgccg tcacgctgca
     9001 aagtggtcag gaagtcaatc ctgaattcag gggctttcgg attcaggtac gtagcgccag
     9061 tcttaccgcc aaatgtcgaa attggaagaa agcccatctc cagcgattcg agcgctttgt
     9121 ggacgtcaac ttgaattgtc gctggtagcg ccagcgagat gttcttgccc gcatgcgcaa
     9181 aatctacgtc ctgggtcctt gatccatcgt gccaacgtat gcctagaacg ttaccaatgg
     9241 cgaggaaagc atgggttccg atgaggatgc cacccgcgcg gaagaatccg tactcggaga
     9301 gccttcttat gacgcgaaaa tgcctcggta atacgcgttc gcatccgagc gcaatagcag
     9361 aacgagcaag cggctctagt gcctttgctg gattttcttt ggctcgctta atgagggctt
     9421 tgacttcgtc ggtatcggga ccgacataaa gctggcgcac ggttccattt acgtcgcgat
     9481 actggtaata ccaatagagc cgacccttaa cggttttgga agcgaagctt ccagagagat
     9541 tcgctattga acgatgcagt tcctgggcgc gagcggactc gaacaattct gcataagccg
     9601 tctgagccga taaggacagc tcgtcatagc gccgtagtct cgtttctgtg gacgccgcca
     9661 tagttatact acattatata taattgtagt ataaaaacca gattgcttcc ttaagtacat
     9721 gatatttaag ttatactacc acgtcgcacg ttgtagtata actaggactc ctcatagctt
     9781 cgcctggagg aaccgcagca gcctgggagc cagaaatcca ggctggctcc caggctcgtc
     9841 gagcgggtcg ccgatccaga agtgcggcgc gccggcgagc acgacggggc gcacgtagtg
     9901 cccggcctgc ttcagcgcct cgaggaaggc gccgctctgc tccttcgcat cgaccacgtc
     9961 gtcgtcgctg ccccagacga cgaggaaggc ggtcgcgttg ttgcgcgccg agacgtacga
    10021 gagcggcgac gcctcgaagt acggccgccg gtcgtcgatc agcgagacgc ccaggaagcg
    10081 atcgacgatc gggtcgcgcg ggcgcgcgag ctggtcgtgg cgccattgcg cgaccaggtc
    10141 gaagacgccg tagacgacga ccgcggcgcg cacgcgcgtc gacacgccgc cgtgcgggtc
    10201 ggcgcggttg ccgtccttga aggccgggtg atcgcccgcg agcgccacca gtgacgcgag
    10261 gtgcgcgccg gaggagtcgc ccatgagcgc gatgcgctcg ggatcgatgc cgagctcggc
    10321 ggcgttggcg cgcgcgtact gcaccgccgc gcgcacgtcg tgcacggctt cggggtacac
    10381 gcgggtcgac gcgctcgaga gccggtgcgt caccgcgagc accgcgtagc cacgctcggc
    10441 gagccaggga ccgaggtgcg cgtagttctc gaggttggcg agccgccagc cgccgccgtg
    10501 caccgcgatc acgacgggca ctttgccgcc gccgcgcggc gcatacaggt gtcccttcag
    10561 cgccgcgccg ccgtggacgg cgtactcgat c
//
LOCUS       DATDMW010000006        13305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2241857, whole genome shotgun sequence.
ACCESSION   DATDMW010000006 DATDMW010000000
VERSION     DATDMW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241857"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VI319_00195"
     CDS             complement(<1..181)
                     /locus_tag="VI319_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6862294.1"
                     /translation="MDTIFGIPTQALFGQLLIGLINGSFYALLSLGLAVIFGLLNIIN
                     FTHGAQYMMGAFCAFI"
     gene            complement(201..1412)
                     /locus_tag="VI319_00200"
     CDS             complement(201..1412)
                     /locus_tag="VI319_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862295.1"
                     /translation="MIPKRAWLAVALLLAATGAQAQISGGVVKIGVLSDMSSLYSDIG
                     GPGSVAAARMAVEDFAPAKHGLKVEIVSADHQNKPDVGSAIARNWYDTEGVDVIVDVP
                     TSSVALAVSQVTKDKNKVFLVSGAASSDLTGKACTPNTIHWTYDTWMLANGTGNAIVK
                     QGGDTWFFITADYAFGHALERDTSEVVKKAGGKVLGAVRHPLNTQDFSSFLLQAQASK
                     AKIIGLANAGGDTTNAIKQAAEFGIVKGGQNLAGLLVFLSDVHGLGLDKAQGLIFTET
                     FYWDMNAQTRAFAKRMAAANNGKYPTMVQAGVYSAITHYLKAVAAGKTDADGAKVVAR
                     MKSMPTDDPLFGKGSIRADGRKLHPAYLVEVKKPSESKGPYDYYKIRATIPADQAFRP
                     LDQGDCPLVKK"
     gene            complement(1449..2141)
                     /locus_tag="VI319_00205"
     CDS             complement(1449..2141)
                     /locus_tag="VI319_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862296.1"
                     /translation="MLKVSGLNAWYGESHILHGIDFEVRRGEVVTLLGRNGAGKTTTL
                     KSIMGMVGRREGSVVFEATETIRMPSYRIARLGLAFCPEERGIFASLDVRENLLLPPA
                     VRPGGFTVEQVYELFPNLKERLRSQGSKLSGGEQQMLAIGRILRTGARLLLLDEPTEG
                     LAPVIVQQIGQTISRLKAEGFTILLVEQNFRFASRVADRHYVIENGRAVDMIPNAELE
                     SRLHEVHEYLGV"
     gene            complement(2155..2910)
                     /locus_tag="VI319_00210"
     CDS             complement(2155..2910)
                     /locus_tag="VI319_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862297.1"
                     /translation="MTADIILETRDLSKEFKGFVAVNGVNLKVKRGTIHALIGPNGAG
                     KTTCFNLLTHFLTPTRGQIVYNGRDITGASPASIARMGLARSFQISAVFPHLTVLENV
                     RLALQRRRGESFDFWRSERVLEELNARARELLDAVGLAAHEATVAVELPYGRKRALEI
                     ATTLALDPELMLLDEPTAGMAHEDVERVAALVKKVAANRTVLMVEHNLGVVEHLSDHI
                     TVLARGEVLAEGDYAAVSKDRNVIAAYLGSERG"
     gene            complement(2986..3789)
                     /locus_tag="VI319_00215"
     CDS             complement(2986..3789)
                     /locus_tag="VI319_00215"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY6862298.1"
                     /translation="MNAPTKTEVEAPYVLTQSKDGVVTLTMNRGERMNPLSTGMLAAL
                     QAELDRLKEDRDAKVVVLAGAGSHFCAGHDLREMRAHPDKAWQRGLFDACSAMMLSLT
                     RLPQPVIARVQGIAVAAGCQLVSMCDLAVASEAARFALPGIKSGIFCTTPGVGVARNL
                     ARKHALEMLFTGDLIDAGTALSWGLVNRVVAADALDAETAKLAAKIVAHSGAVVAMGK
                     RFFYDQVEKGLADAYSLASEGMACNMMLADAAEGIDAFIGKRKPEWRDK"
     gene            complement(3815..5452)
                     /locus_tag="VI319_00220"
     CDS             complement(3815..5452)
                     /locus_tag="VI319_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY6862299.1"
                     /translation="MANPYEVDLERNPANHAPLTPLGFIERAAAVYPKRTAVIHGERR
                     FTWAETYARSRRLASALAARRLGRGDTVAVMLSNTPEMVECHFGVPMAGTVLNALNTR
                     LDADAIAFMLEHGGAKLVIVDREYSEVMAKALAMLKSRPPVIDVDDPEYSGPGKRIGE
                     IDYESFLAKGDPEFAWSPPRDEWDAISLNYTSGTTGNPKGVVYHHRGAYLNAVCNIVT
                     WAMPHHAVYLWTLPMFHCNGWCFPWTVAANAGVNVCLRKVEAKAIFDLIRRHRVTHYC
                     GAPIVHSTLMHADPKLRAGISHPVHAMVAAAAPPAAMIEGMERMGWDLTHVYGLTEVY
                     GPATVCAKHDEWKSLDIGKRTERMGRQGVRYLMEEGVAVMDPETMQPVPADGETMGEI
                     MFRGNITMKGYLKNKQASEEAFKGGWFHSGDLAVLQPDGYVKIKDRAKDIIISGGENI
                     SSLEVEDVLYRHPAVLAAAVVAKPDPKWGETPLAFVELKPEANVSEEELIEHCRAHLA
                     RFKAPRQVVFGPLPKTSTGKIQKFMLRQKAKSTSAIE"
     gene            complement(5463..6275)
                     /gene="dnaQ"
                     /locus_tag="VI319_00225"
     CDS             complement(5463..6275)
                     /gene="dnaQ"
                     /locus_tag="VI319_00225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY6862300.1"
                     /translation="MRCIVLDTETTGLNASLGDRIIELGCVEMLNRRPTGNDFHRYVN
                     PERASEAGARQVHGIDDEFLRDKPKFGEIATELLEYLRGAEVVIHNAAFDIEFLNNEL
                     KLLGLPLIEPGKPNDYGITVIDSLALARELHPGKKNSLDALCERYAVDNAHRTLHGAL
                     LDARLLADVYLAMTRGQDSLAIELEAPRAAVSAGAGALAAARPALVVLAASAEELAEH
                     EKLLAAIDAESKGRCVWKLLDAAPVPLEPEVPLAGVMPINPAVSRAPGPAPL"
     gene            complement(6346..7434)
                     /locus_tag="VI319_00230"
     CDS             complement(6346..7434)
                     /locus_tag="VI319_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY6862301.1"
                     /translation="MATTGKKNSARAEPFARIGAKAKRAIEHVVIIVKENHGFDNYFG
                     TFPGADGAALPRSPNPPPHDPNHRHDAWLTRKTTAVRAQFTEQDIPAYFAWARQFTLC
                     DRYFSEIAGPSTPNHLTLIAADSPIFVNPPRYRIPVGEPLFDIPSLPAALEKAGLTWG
                     NYGGYAFDFIKSLVNSKRKFTSDQFAKDAAKGKLPNVSWVYAPHDASEHPPDENDAGT
                     PLVGNVTHGMQWTVDQVDALVQGGLWPKTAIFVTWDDWGGWLDHVDPPEVETWKHDPA
                     HPERDGNHPKWDGTQFRYGSRVGCLVLSPYAKSGHVSKAVHSHVSLLRFCEKLFGLPA
                     LNTRDAQASDMSDCFDFRRAPAPPPKHG"
     gene            complement(7498..8115)
                     /locus_tag="VI319_00235"
     CDS             complement(7498..8115)
                     /locus_tag="VI319_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY6862302.1"
                     /translation="MRRTKEESERTRLQILAAARRVFARQGVTRTSLEQIARAAGVTR
                     GAIYWHFADKTALFYAMREQVSLPMVDRTGFAWFPADTADPLASVERFLLEVLRTLES
                     DPATRETFQIMGFKCEYVGDLERELNLHNSRCTELYTMLSGVYRRAERARQLRKGLKP
                     APAALSTCSFLVGLVRLWLMDRTGKLVRSKAKSIVKAHVDSLRRA"
     gene            8207..9439
                     /locus_tag="VI319_00240"
     CDS             8207..9439
                     /locus_tag="VI319_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6862303.1"
                     /translation="MNRGPSLLISVLAAASLIGPLAGCDRGDAAVRQAGAQQGPPPVP
                     AKVREVKPQPVPIVLDAVGQVQGSKEVEVRARVTGILLKRLYNEGQRVQAGAPLFQID
                     PAPYEIALQQARAQLAQEQARQEQTKREAGRLKDLAEQKAISQKEFDDAVSAQKLSIA
                     SLQAAEARVREAELNLSYTHVTAPVSGVTGRIARSEGSLVSPGADSLLTTVIQVQPVW
                     VRFSLSESDLAKLPGRRVDPSATQVQLVLADGSVFPTKGRINFAAAQIDPRLATQELR
                     AEFDNPKSRILPGDFVRVRLTGGEYKNAYLVPQQAVFQTEQGQFVFVLDGENKATVRP
                     VKTGEWIGQDWLVLDGLRPGDRVVVDNLMKLRPGAPVAPQLADAGSAPAPGGPAGNAA
                     GAPKQQSSPAAGKTPASK"
     gene            9449..12577
                     /locus_tag="VI319_00245"
     CDS             9449..12577
                     /locus_tag="VI319_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY6862304.1"
                     /translation="MLSKYFIERPIFASVIAIIITIAGLVASQVLPIAQYPEISPPTV
                     TITATYPGASAETLARTVAAPIEEQLSGVEGLLYYQSSSSSTGTLTITATFDVGTDIN
                     NATIQVNNRVQIALPRLPDDVRRTGVLVQKRSIDILLVVGVTSNDPRYDTLFLSNYML
                     INALDVLKRVPGVADATIFGARDYSMRIWLKPDRMAQLGITPTDVANAIRVQNNQYAA
                     GKIGQEPAPPDQALNFTVTTRGRLVEPEEFGNIVLRSSGPGGVLRVKDVARVELGAAN
                     YDAFTTVDGNPSIGVAVFLQSGANAIEVAKKVRTAMNEVAKGFPAGVSYIIPFDTTHF
                     VQESIHEVIITLLIAAVLVLGVVFLFLQSWRATLIPFIAVPISLIGSFAGLYLFGFSI
                     NTLTLFAIVLATGIVVDDAIVVLENVERVMAEKKLSPHDAAVESMREVTGAIIAIVLV
                     LCSVFVPVAFLGGLAGQLYKQFAVTVATAVVISGFIAVTLTPALCALLLKPTHEENRL
                     FRPFNRGFERAVGWYTGGVRLTIRHAVWALVAFAAVIVIVLGLFRIVPSSLVPSEDQG
                     YLFGAIVLPDGATLSRTGRVASQIQKAIQEHPAVEHVFAVTGFDLIGGGNKTSAGTLF
                     VTLKPWRDRKESAADVVRYVSMKGFGLNEGLVFAFNPPPIRGLGTAGGFEAYIQNRSN
                     ADPQQLNQVLQQFMGELRKQPDLAGINSFFRPAVPQLLVDVDREKALSLGVPLSDVFD
                     ALQSTMGALYVNDFNKFGRTYRVQVQADPQYRIRPEDLGNVYVRSASSGEMVPIKSLV
                     RVQSIVGPDQLDRYNGFVAAKVLGGAAPGVSSGEAIAKVEQVAAQALPPGYTLEWTGQ
                     AFQEKRTGQASIIAFCFAIVMVFLILSANYERWSLPVAVLLAVPFALLGALAAVLARG
                     MTNDVYFQIGLVTLIGLAAKNAILIVEFAQQNALQGMSLYDAAITAARQRFRPIVMTS
                     LAFVLGVVPLVVATGAGAAARQSMGTGVFGGMLAATFIATLFVPWFFIVLAGLRKKRR
                     ERLQARPGETHA"
     gene            12574..>13305
                     /locus_tag="VI319_00250"
     CDS             12574..>13305
                     /locus_tag="VI319_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6862305.1"
                     /translation="MTPRLAAPALAAALLAGCMVGPDYERPTMELPAAYPGPKAAPTE
                     AQAPAQQAPADAQVPADWWKLYNDPLLDDLVASALARNADIRQAVARIEESDANLREA
                     NAAFLPEIDAQGNAGRSRASTTTGLIPPGIPSIRTDIRLALTTAFELDFWGRLRRGVE
                     ALRAQALASRYARDVVALTLAGLTTQAYFGVRSLDAQILVTRETLANRDESLAYVRKR
                     AAGGIASDLEVAQAETARAAIAVQLR"
BASE COUNT         1953 a         4673 c         4513 g         2166 t
ORIGIN      
        1 ggatgaacgc gcagaacgcg cccatcatgt actgcgcgcc gtgcgtgaag ttgatgatgt
       61 tgagcaggcc gaagatcacg gccagcccga gcgagagcag cgcgtagaac gagccgttga
      121 tcagtccgat caggagctga ccgaagagcg cctgggtggg gatcccgaag atcgtgtcca
      181 tcgccgggat ccccgacctc ttatttcttc accagcgggc aatccccctg gtcgagcggc
      241 cggaacgcct ggtcggccgg gatggtcgcg cggatcttgt agtagtcgta cgggcccttc
      301 gactcggacg gcttcttcac ctcgacgagg tacgccggat ggagcttgcg gccgtcggcg
      361 cggatgctgc ctttgccgaa gagcgggtca tcggtcggca tcgacttcat ccgggcgacg
      421 accttcgcgc cgtcggcatc cgtcttgccc gccgcgaccg ccttcaggta gtgcgtgatc
      481 gccgagtaca cgccggcctg caccatggtg gggtacttgc cgttgttggc cgcggccatg
      541 cgcttggcga aggcgcgcgt ctgcgcgttc atgtcccagt agaacgtctc ggtgaagatc
      601 agcccctgcg ccttgtcgag ccccaggccg tgcacgtcgg acaggaatac cagcagcccg
      661 gcgaggttct gcccgccctt gacgatgccg aactcggcgg cctgcttgat cgcgttggtc
      721 gtgtcgccgc ccgcattggc gaggccgatg atcttggcct tcgacgcctg cgcctgcagc
      781 aggaacgacg agaagtcctg ggtgttgagc ggatggcgaa ccgcgcccag caccttgccg
      841 ccggccttct tcaccacctc ggacgtgtcg cgctcgagcg catggccgaa ggcgtagtcg
      901 gcggtgatga agaaccaggt gtcgccgccc tgcttcacga tggcgttgcc ggtgccgttc
      961 gcgagcatcc acgtgtcgta ggtccagtgg atggtgttcg gcgtgcacgc cttgcccgtg
     1021 aggtcggagc tcgccgcgcc ggagacgagg aacaccttgt tcttgtcctt ggtcacctgg
     1081 ctgaccgcga gcgcgaccga cgaggtcggc acgtcgacga tcacgtcgac cccctcggtg
     1141 tcgtaccagt tccgcgcgat cgccgagccg acgtcgggct tgttctggtg gtcggccgag
     1201 acgatctcga ccttcaggcc gtgcttcgcg ggcgcgaagt cctcgaccgc catgcgcgcc
     1261 gcggcgaccg agccgggccc gccgatgtcg gaatacaggc tggacatgtc ggacagcacg
     1321 ccgatcttca cgacgccgcc ggaaatctgc gcctgcgcgc cggtcgccgc cagcagcagc
     1381 gccaccgcaa gccatgctcg cttgggaatc atcgtcctcg ctcctttgat tgggaaaccc
     1441 tcccgggatc acacgcccag gtactcgtgg acctcgtgca gccgcgactc tagctcggcg
     1501 ttcgggatca tgtccaccgc ccgcccgttc tcgatcacgt aatggcggtc ggcgacgcgc
     1561 gacgcgaagc ggaagttctg ctcgaccagc aggatcgtga acccctccgc cttcagccgc
     1621 gagatcgtct gcccgatctg ctgcacgatc acgggcgcga ggccttcggt cggctcgtcg
     1681 aggagcagca gccgcgcccc ggtgcgcagg atgcgcccga tcgcgagcat ctgctgctcg
     1741 ccgcccgaga gcttgctacc ctggctgcgc agccgctcct tcaggttggg gaagagctcg
     1801 tacacctgct cgacggtgaa gccgccgggg cgcaccgcgg gcggcagcag caggttctcg
     1861 cgcacgtcga ggctcgcgaa gatgccgcgc tcctcggggc agaaggcaag ccccaggcgc
     1921 gcgatgcggt acgacggcat gcggatcgtc tcggtcgcct cgaagacgac cgagccctcg
     1981 cgccggccca ccattcccat gatcgacttc agcgtggtgg tcttccccgc cccgttgcgc
     2041 cccagcagcg tcacgacctc gccgcggcgc acttcgaaat cgattccgtg caggatgtgc
     2101 gactcgccgt accaggcgtt caggcccgac actttcagca tcggcgccgt tgcgtcagcc
     2161 acgctcgctc ccgagatacg ccgcgatcac gttgcggtcc ttcgacacgg ccgcgtagtc
     2221 gccctccgcc agcacctcgc cgcgcgcgag cacggtgatg tggtcggaga ggtgctcgac
     2281 cacgccgagg ttgtgctcga ccatgagcac ggtgcggttg gccgcgacct tcttgaccag
     2341 cgccgcgacg cgctccacgt cctcgtgcgc catgcccgcg gtcggctcgt cgagcagcat
     2401 cagctccggg tcgagcgcga gcgtggtggc gatctcgagc gcgcgcttgc gcccgtaggg
     2461 cagctcgacc gcgaccgtcg cctcgtgcgc cgcgaggccg accgcgtcga gcagctcgcg
     2521 cgcgcgcgcg ttcagctcct cgagcacgcg ctcggagcgc cagaagtcga acgactcgcc
     2581 gcgccggcgc tgcagcgcga ggcgcacgtt ctcgagcacc gtgaggtgcg gaaacacggc
     2641 ggagatctgg aacgagcgcg ccaggcccat gcgcgcgatc gacgccgggc tcgcgccggt
     2701 gatgtcgcgg ccgttgtaga cgatctggcc gcgcgtcggg gtcaggaagt gcgtgaggag
     2761 gttgaagcac gtggttttcc ccgccccgtt gggcccgatg agcgcgtgga tggtcccgcg
     2821 cttcaccttg aggttcacgc cgttgaccgc gacgaagccc ttgaactcct tcgacaggtc
     2881 ccgggtctcg aggatgatgt ccgctgtcat tctgaggagc gaagcgacga agaatctgct
     2941 ttttaccgca aaagcagatc cttcgcttcg ctcaggacga cagtcctact tatcgcgcca
     3001 ttcgggcttg cgcttgccga tgaacgcatc gatcccctcg gcggcgtccg cgagcatcat
     3061 gttgcaagcc atcccctcgg aggcgagcga gtaggcgtcg gcgagcccct tctcgacctg
     3121 gtcgtagaag aagcgcttgc ccatcgcgac cacggcgccg ctgtgcgcga cgatcttcgc
     3181 ggcgagcttc gccgtctcgg cgtcgagcgc gtcggccgcg acgacgcggt tcaccaggcc
     3241 ccacgaaagc gccgtgccgg cgtcgatcag gtcgccggtg aagagcatct cgagcgcgtg
     3301 cttgcgcgcg aggttgcggg cgacgcccac gccgggcgtg gtgcagaaga tgccgctctt
     3361 gatcccgggc agcgcgaagc gcgcggcctc cgacgccacc gcgaggtcgc acatcgagac
     3421 cagctggcac cccgccgcga ccgcgatgcc ctgcaccctc gcgatgaccg gctgcggcag
     3481 ccgcgtcagc gacagcatca tcgcgctgca cgcatcgaac agcccgcgct gccacgcctt
     3541 gtccggatgc gcgcgcatct cccgcaggtc gtgccccgcg caaaaatggc tgcccgcccc
     3601 ggcgagcacc acgaccttcg catcgcgatc ctccttcagg cgatcgagct cggcctgcag
     3661 cgcggcgagc attccggtcg acagcgggtt catgcgctcg ccgcggttca tcgtgagagt
     3721 caccacgccg tccttcgact gggtcaggac gtacggcgct tcgacttcag tcttcgtcgg
     3781 tgcattcatg acccgctccg caggatgacg atcatcactc gatcgccgac gtggacttcg
     3841 ccttctgccg cagcatgaac ttctggatct tgccggtcga ggtcttgggc agcgggccga
     3901 agacgacctg gcggggcgcc ttgaaccgcg ccaggtgcgc gcggcaatgc tcgatcagct
     3961 cctcctcgct cacattcgct tccggcttca gctcgacgaa cgcgagcggc gtctcgcccc
     4021 acttcgggtc gggcttggcg accaccgcgg cggcgagcac cgcgggatgg cggtagagca
     4081 cgtcctcgac ctcgagcgag gagatgttct cgccgcccga gatgatgatg tccttggcgc
     4141 ggtccttgat cttgacgtac ccgtcgggct gcagcacggc gaggtcgccc gagtggaacc
     4201 agccgccctt gaatgcctcc tcgctcgcct gcttgttctt gaggtagccc ttcatggtga
     4261 tgttgccgcg gaacatgatc tcgcccatgg tctcgccgtc ggcgggcacg ggctgcatcg
     4321 tctcggggtc catcaccgcg acgccttcct ccatcaggta gcgcacgccc tggcgcccca
     4381 tgcgctcggt gcgcttgccg atgtcgaggc tcttccattc gtcgtgcttc gcgcacacgg
     4441 tcgccggacc gtagacctcg gtgaggccgt agacgtgcgt caggtcccag cccatgcgct
     4501 ccatgccctc gatcatcgcg gcgggcggcg ccgccgcggc caccatggcg tgcaccgggt
     4561 gcgagatccc cgcgcgcagc ttcggatcgg cgtgcatcag cgtcgagtgc acgatcggcg
     4621 cgccgcagta gtgcgtgacg cggtggcggc ggatcaggtc gaagatcgcc ttcgcctcga
     4681 ccttgcgcag gcagacgttg acgcccgcgt tcgcggcgac ggtccacggg aagcaccagc
     4741 cgttgcagtg gaacatcggc agcgtccaca ggtacaccgc gtggtgcggc atcgcccagg
     4801 tgacgatgtt gcagaccgcg ttcagatacg cgccgcggtg gtggtagacg acgcccttcg
     4861 ggttgccggt cgtgcccgag gtgtagttga gcgagatcgc gtcccactcg tccctcgggg
     4921 gcgaccacgc gaattccggg tcgcctttcg cgaggaagga ctcgtagtcg atctcgccga
     4981 tgcgcttgcc ggggccggag tactccgggt cgtcgacgtc gatcaccggc ggcctggact
     5041 tcagcatcgc cagcgccttc gccatcacct cggaatactc gcggtcgacg atgacgagct
     5101 tcgccccccc gtgctcgagc atgaacgcga tcgcatcggc gtcgagccgc gtgttcagcg
     5161 cgttcagcac cgtgcccgcc atcggcacgc cgaaatggca ctcgaccatc tcgggcgtgt
     5221 tcgacagcat caccgccacc gtgtcgccgc gcccgagccg gcgcgccgcg agcgcggagg
     5281 cgagccggcg cgagcgcgcg taggtctcgg cccatgtgaa gcggcgctcg ccgtggatca
     5341 cggcggtgcg cttcgggtag accgccgccg cccgctcgat gaagccgagc ggcgtcagcg
     5401 gagcatggtt cgcgggattg cgctcgagat cgacttcgta ggggttcgcc acgaccccga
     5461 ttttacaagg gcgcgggccc cggtgcgcgc gagacggccg ggttgatcgg catcacgccg
     5521 gcgagcggga cctcgggctc gagcgggact ggcgccgcgt cgagcagctt ccagacgcag
     5581 cgccccttgc tctcggcgtc gatcgcggcg agcagcttct cgtgctcggc gagctcctcg
     5641 gcgctcgcgg cgagcaccac cagcgccggg cgcgcggcgg cgagcgcgcc cgccccggcc
     5701 gagaccgcgg cgcggggcgc ctcgagctcg atcgccaggc tgtcctggcc gcgcgtcatc
     5761 gcgaggtaga cgtcggcgag caggcgcgcg tcgagcagcg cgccgtgcag cgtgcggtgg
     5821 gcgttgtcca ccgcgtagcg ctcgcacagg gcgtcgagcg agttcttctt gccgggatgc
     5881 agctcgcgcg cgagcgcgag cgagtcgatc acggtgatgc cgtagtcgtt gggcttgccc
     5941 ggctcgatga gcggcaggcc cagcagcttc agctcgttgt tcaggaactc gatgtcgaag
     6001 gccgcgttgt ggatcacgac ctccgcgccg cggagatact ccaggagctc ggtcgcgatc
     6061 tcgccgaatt tcggcttgtc gcgcaggaac tcgtcgtcga tgccgtggac ctgccgcgcc
     6121 cccgcctcgc tggcgcgctc ggggttcacg tatcggtgga aatcgttgcc ggtcggccgc
     6181 cggttgagca tctcgacgca gccgagctcg atgatccggt cacccaggct ggcgttcaac
     6241 ccggtggtct cggtgtcgag cacgatacag cgcattcccc tgcctcgttc cacgttcttg
     6301 ttctgccgga cgaagcgtaa accggccggc ggaataaagc aaaggctatc cgtgcttcgg
     6361 cggcggcgcc ggcgcgcggc ggaagtcgaa gcagtcggac atgtcgctcg cttgagcgtc
     6421 gcgcgtgttg agcgccggaa gcccgaagag cttctcgcag aagcgcagca ggctcacgtg
     6481 cgagtgcacc gccttggaca cgtggccgct cttcgcgtag ggcgagagca ccaggcagcc
     6541 gacgcgcgag ccgtagcgga actgcgtgcc gtcccacttc ggatgattgc cgtcgcgctc
     6601 cgggtgcgcg ggatcgtgct tccaggtctc gacctccggc gggtcgacgt ggtccagcca
     6661 tccgccccag tcgtcccagg tcacgaagat cgcggtcttc ggccagaggc cgccctgcac
     6721 cagagcgtcg acctggtcga cggtccactg catgccgtgc gtgacattgc cgacgagcgg
     6781 cgtgccggcg tcgttttcgt cgggcggatg ctcgctcgcg tcgtgcggcg cgtagaccca
     6841 ggacacgttc ggaagcttgc ccttcgccgc gtccttcgcg aactggtcag acgtgaactt
     6901 gcgcttgctg ttcaccaggc tcttgatgaa gtcgaacgcg tagccgccgt agttgcccca
     6961 cgtgagcccc gccttctcga gcgcggcggg cagcgacgga atgtcgaaca gcggctcgcc
     7021 gaccgggatc cggtaccgcg gcggattgac gaagatcggc gaatccgccg cgatcagcgt
     7081 caggtgattc ggcgtcgaag gccccgcgat ctcggaaaaa tagcggtcgc acagcgtgaa
     7141 ctggcgcgcc caggcgaagt acgccgggat gtcctgctcg gtgaactgcg cgcgcaccgc
     7201 cgtcgtcttg cgcgtcagcc aggcgtcgtg gcggtggttc ggatcgtgcg gcggcggatt
     7261 gggcgagcgc gggagcgccg cgccgtcggc gccggggaac gtcccgaagt agttgtcgaa
     7321 gccgtggttc tccttgacga tgatcaccac gtgctcgatg gcgcgcttcg ccttcgcgcc
     7381 gatgcgggcg aacggctccg cgcgcgcgct gttcttcttg ccggtggtgg ccatgcctcc
     7441 tcccggagtt ggtgcgccga ttctgacgcc ggccggctga cggccgcctg actcgcgcta
     7501 cgcgcgccgc aggctgtcga cgtgggcctt gacgatggac ttcgccttgc tgcgcacgag
     7561 cttgccggtg cggtccatga gccacaggcg caccaggccc acgaggaagc tgcaggtcga
     7621 gagcgcggcc ggcgcgggct tgaggccttt acgcagctgg cgcgcgcgct cggcgcggcg
     7681 gtacacgccg ctcagcatcg tgtagagctc ggtgcagcgg ctgttgtgca ggttcagctc
     7741 gcgctcgagg tcgccgacgt actcgcactt gaagcccatg atctgaaagg tctcccgcgt
     7801 cgcggggtcg ctctccaggg tgcgcagcac ctcgagcagg aagcgctcga cgctggcgag
     7861 cgggtcggcc gtgtccgccg ggaaccacgc gaagccggtg cggtcgacca tcggcaggga
     7921 cacctgctcg cgcatcgcgt agaacagcgc ggtcttgtcc gcgaagtgcc agtagatcgc
     7981 gccgcgcgtc acgccggcgg cgcgcgcgat ctgctcgagg ctggtgcggg tcacgccctg
     8041 gcgcgcgaat acccggcgcg cggccgccag gatctggagg cgcgtccgct cggattcttc
     8101 cttggttctg cgcaagctat cgggtcctgc gagttgacat acatccatga ttgtataaaa
     8161 ttgtgacttc ttcgacaacc atgatcgcgg acctcgccca accatgatga accgcggccc
     8221 gtcgctcctc atctccgtgc tcgccgccgc atcgctgatc ggcccgctgg ccgggtgcga
     8281 ccgcggcgac gctgccgtcc gccaggccgg cgcgcagcag ggtccgccgc ccgtcccggc
     8341 caaggtgcgc gaggtcaagc cgcagcccgt tccgatcgtg ctcgatgcgg tcggccaggt
     8401 gcagggctcg aaggaagtcg aggtgcgcgc gcgcgtcacc ggcatcctcc tcaagcgcct
     8461 ctataacgag ggccagcgcg tgcaggccgg cgctcccctc ttccagatcg accccgcgcc
     8521 gtacgagatc gcgctgcagc aggcgcgcgc gcagctcgcg caggagcagg cgcgccagga
     8581 gcagaccaag cgcgaggccg ggcgcctcaa ggatctcgcc gagcagaagg cgatcagcca
     8641 gaaggagttc gacgacgcgg tgtcggcgca gaagctgtcg atcgcgtcgc tgcaggccgc
     8701 ggaggcgcgc gtgcgcgagg ccgagctgaa cctctcgtat acgcacgtga ccgcgcccgt
     8761 gtcgggcgtg accggccgca tcgcgcgctc cgagggcagc ctcgtctcgc cgggcgccga
     8821 cagcctgctc acgaccgtga tccaggtgca gccggtgtgg gtgcggttca gcctgtcgga
     8881 gtccgacctg gcgaagcttc cggggcggcg cgtcgacccg agcgctaccc aggtgcagct
     8941 cgtcctcgcc gacggctcgg tcttcccgac caaggggcga atcaatttcg ccgccgcgca
     9001 gatcgatccg cgactcgcga cgcaggagct gcgcgccgag ttcgacaacc cgaagagccg
     9061 catccttccc ggagatttcg tgcgcgtgcg cctcaccggg ggcgagtaca agaacgccta
     9121 cctcgtgccg cagcaggcgg tgttccagac cgagcaggga cagttcgtgt tcgtcctcga
     9181 cggagagaac aaggcgaccg tgcggccggt gaagaccggc gagtggatcg gccaggactg
     9241 gctcgtcctc gacggcctcc ggcccggcga ccgcgtcgtc gtcgacaacc tcatgaagct
     9301 ccggccgggc gcgccggtcg cgccgcagct cgccgacgcc ggctccgcgc cggcgccggg
     9361 cggcccggcg ggcaacgccg ccggcgcgcc gaagcagcag tcgtctcccg ccgccggcaa
     9421 aacacccgcg tccaagtaag cgccggccgt gctctcgaaa tacttcatcg agcggccgat
     9481 ctttgcgtcg gtcatcgcca tcatcatcac gatcgccggc ctggtcgcgt cgcaggtgct
     9541 gccgatcgcg cagtacccgg agatttcgcc gcccacggtg acgatcacgg cgacttaccc
     9601 cggcgccagc gccgagacgc tcgccaggac cgtcgccgcg ccgatcgagg agcagctctc
     9661 gggcgtcgag ggcctgctct actaccagtc gagctcgtcg tcgaccggca cgctgacgat
     9721 caccgcgacc ttcgacgtcg gcaccgacat caacaacgcc accatccagg tcaacaaccg
     9781 ggtgcagatc gcgctgccgc ggctgcccga cgacgtgcgc cgcacgggcg tgctggtcca
     9841 gaagcgctcg atcgacatcc tgctcgtcgt cggcgtgacc tcgaacgacc cgcgctacga
     9901 cacgctcttc ctcagcaact acatgctgat caacgccctc gacgtgctca agcgcgtccc
     9961 gggcgtcgcc gacgccacca tcttcggcgc gcgcgactac tcgatgcgca tctggctcaa
    10021 gcccgaccgc atggcgcagc tcggcatcac gccgaccgac gtcgccaacg cgatccgcgt
    10081 ccagaacaac cagtacgcgg ccgggaagat cggccaggag cccgccccgc ccgaccaggc
    10141 gctcaacttc acggtgacga cgcgcggccg gctggtcgag cccgaggagt tcggcaacat
    10201 cgtgctgcgc tcgagcggcc ccggcggcgt gctgcgcgtc aaggacgtcg cgcgcgtcga
    10261 gctgggggcc gcgaactacg acgcgttcac caccgtcgac ggcaacccga gcatcggcgt
    10321 cgccgtgttc ctgcagtcgg gcgcgaacgc gatcgaggtc gccaagaagg tgcgtacggc
    10381 gatgaacgag gtcgcgaagg ggttcccggc gggcgtgagc tacatcatcc cgttcgacac
    10441 cacgcacttc gtccaggaat cgatccacga ggtgatcatc acgctgctga tcgcggcggt
    10501 gctcgtgctc ggcgtcgtgt tcctgttcct gcagagctgg cgcgcgacgc tgatcccgtt
    10561 catcgcggtg ccgatctcgc tgatcggctc cttcgccggc ctgtacctgt tcggcttctc
    10621 gatcaacacg ctgacgctgt tcgcgatcgt gctcgcgacc ggcatcgtgg tcgacgacgc
    10681 gatcgtcgtg ctcgagaacg tcgagcgcgt gatggccgag aagaagctct cgccgcacga
    10741 cgcggcggtc gagtcgatgc gcgaggtcac gggcgcgatc atcgccatcg tgctggtgct
    10801 gtgctcggtg ttcgtcccgg tcgcgttcct cggcggcctc gcgggccagc tgtacaagca
    10861 gttcgccgtc acggtcgcga cggcggtcgt gatctcgggc ttcatcgcag tgaccctcac
    10921 cccggcgctg tgtgcgctgc tgctcaagcc gacgcacgag gagaaccgcc tgttccgccc
    10981 gttcaaccgc ggcttcgagc gcgcggtcgg ctggtatacc gggggcgttc gcctcaccat
    11041 ccgacacgcg gtgtgggcgc tggtcgcgtt cgccgcggtg atcgtgatcg tgctggggct
    11101 gttccgaatc gtgccgagca gcctggtgcc ctccgaggac cagggctatc tcttcggcgc
    11161 catcgtgctg cccgacgggg cgacgctctc gcgcaccggc cgcgtcgcct cgcagatcca
    11221 gaaggcgatc caggagcacc cggcggtcga gcacgtgttc gccgtcacgg gcttcgacct
    11281 gatcggcggc ggcaacaaga ccagcgcggg cacgctgttc gtgacgctca agccgtggcg
    11341 ggaccgcaag gagagcgccg ccgacgtcgt gcgctacgtc agcatgaaag gcttcggcct
    11401 caacgagggc ctggtgttcg cgttcaaccc gccgccgatc cggggcctcg gcaccgcggg
    11461 cggcttcgag gcctacatcc agaaccgctc caatgccgat ccgcagcagc tcaaccaggt
    11521 gctgcagcag ttcatgggcg agctgcgcaa gcagcccgac ctcgccggca tcaacagctt
    11581 cttccggccg gcggtgccgc agctgctggt cgacgtcgac cgcgagaagg cgctgtcgct
    11641 cggcgtgccg ctgtcggacg tgttcgacgc gctgcagagc acgatgggcg cgctctacgt
    11701 gaacgacttc aacaagttcg ggcgcaccta ccgcgtgcag gtgcaggccg acccgcagta
    11761 ccgcatccgc cccgaagacc tggggaacgt ctacgtccgc tcggcctcga gcggcgagat
    11821 ggtgccgatc aagtcgctgg tgcgggtgca gagcatcgtc gggcccgacc agctcgaccg
    11881 ctacaacggc ttcgtcgcgg ccaaggtgct cggcggcgcc gcgcccggag tgagctcggg
    11941 cgaggcgatc gccaaggtcg agcaggtggc cgcgcaggcg ctcccgcccg ggtacacgct
    12001 cgagtggacc gggcaggcct tccaggagaa gcgcacggga caggcgtcga tcatcgcgtt
    12061 ctgcttcgcc atcgtcatgg tgttcctgat cctgtcggcg aactacgagc gctggtcgct
    12121 gccggtggcg gtgctgctcg ccgttccgtt cgcgctgctc ggtgcgctgg ccgccgtgct
    12181 ggcgcgcggg atgaccaacg acgtgtattt ccagatcggc ctggtcacgc tgatcggcct
    12241 cgccgccaag aacgcgatcc tgatcgtcga gttcgcgcag cagaacgccc tgcagggcat
    12301 gtcgctctac gacgccgcga tcaccgcggc gcgccagcgc ttccgcccga tcgtgatgac
    12361 ttcgctcgcc ttcgtgctcg gcgtcgtgcc gctggtggtc gcgacaggcg ccggcgccgc
    12421 cgcgcgccag tcgatgggca cgggcgtgtt cggcggcatg ctcgcggcga ccttcatcgc
    12481 gacgctgttc gtgccgtggt tcttcatcgt gctcgcgggc ctgcggaaaa agcgccgcga
    12541 gcgcttgcag gcgcgtccgg gcgagacgca cgcatgacgc cgcgcctcgc tgcccccgcg
    12601 ctcgccgccg ctctcctcgc cggctgcatg gtcggccccg attacgagcg gccgacgatg
    12661 gagctgcccg ccgcttatcc cggccccaag gccgcgccca ccgaggcgca ggcgccggcg
    12721 cagcaggctc ccgcggacgc gcaagtgccg gccgactggt ggaagctcta caacgatccg
    12781 ctgctcgacg acctcgtcgc ctcggcgctc gcgcgcaacg ccgacatccg ccaggccgtg
    12841 gcccggatcg aggagtccga cgcgaacctg cgcgaggcga acgccgcgtt cctgcccgag
    12901 atcgacgcgc agggcaacgc cgggcgctcg cgcgcgagca ccaccaccgg gctcataccg
    12961 cccggcatcc cctcgatccg caccgacatc cggctcgcgc tcacgaccgc gttcgagctg
    13021 gacttctggg gtcgcctgcg ccgcggcgtg gaagcgcttc gcgcccaggc gctcgcctcc
    13081 cgctacgccc gcgacgtggt cgccctgacg ctcgcagggc tcaccacgca agcctacttc
    13141 ggcgtgcgct cgctcgacgc gcagatcctc gtcacccgcg agacgctcgc caaccgcgac
    13201 gagtcgctcg cctacgtgag aaagcgcgcc gcgggcggaa tcgcctccga cctcgaggtg
    13261 gcccaggccg agacggcgcg cgcggcgatc gccgtgcagc tgcgc
//
LOCUS       DATDMW010000007         4992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_872736, whole genome shotgun sequence.
ACCESSION   DATDMW010000007 DATDMW010000000
VERSION     DATDMW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872736"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VI319_00255"
     CDS             <1..871
                     /locus_tag="VI319_00255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6862306.1"
                     /translation="VALAVRQKFYHRNPSDDVLAGVRREVAGSLVERVLLLKEAKRRG
                     LRPERARVDAALAAYEERYGASPQWPKVKAEALPKLTARLEEDSVLEQLERAVREAPQ
                     PGDKALRAYYLRHPGLFTEPEQVRLSVILLKVDPSSTRAAWDKAEAEARDIVKQLKAG
                     ADFAAIARERSGDAESAKAGGDMGYLHRGMLPEGLHGEVDKLKPGAISQQPVRLLEGV
                     VVLRLEDRREQRLRGFDEVRARAVELVRRERSDANWAALVSSLRKRAVIEIDGSRYPE
                     AAAAVAAARGGAR"
     gene            907..2928
                     /locus_tag="VI319_00260"
     CDS             907..2928
                     /locus_tag="VI319_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862307.1"
                     /translation="MRDGRRSGGARGLIVRLAMAALSCTTPALAATGDPCPEGDGLSL
                     CSPSGARARESWMSAQESGSLDAPIEVPVEPEAAPDAPPGDPGAPPMRGLEWRLAPVR
                     WGGNASYELRRLGVAGHAARLQQVETANLRGASYVWQPWFMQVSGGLGIVAGRERSRP
                     EGDDSTAVGRSNSNTVTGSGALSVFPSSRFPFQASFDKTDSRASGDITSNDFTNTRWG
                     ARQNYRSGAGDLQYTGSWDRGTLESPSFGRDTVDVLSAGVIRSAGPQTLDFSGSRSRN
                     WRGDTGDGSRIERVVARHGYRPDPLAAVETLASSSSSRFDLTANGAPSENRTRFDQAS
                     TFATWRAGQGSPLYLSGGGRVFRSSVDVNGAASDADTLSGNLAASYALSRHTTLNGAV
                     TATRVTTDTAAQSTSNVITTESAGATYVPDATPLGGFLYTWNAAANAANQNGGVAEAD
                     ASGARSRQNYGGQLGHNLTRSAPLSDASSVTYGAGQSYSSIYDTVTERSQTLTHNASV
                     SVSSSPGSAATAFASLYGADARTHGHNENTFQIVNLQVNGQFQLSRWSLANANLTVQG
                     VRQETPGAPPAGFNRSSGGNLTYQHLRAFDLPRLRYTAIYGANQIQYSSRLEGDVNAP
                     RERVTRSFEQRLDWSVGRLDLQLSARTATIDGQRNELVFFRINRQFGAF"
     gene            2954..3352
                     /locus_tag="VI319_00265"
     CDS             2954..3352
                     /locus_tag="VI319_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HEY6862308.1"
                     /translation="MSKRLRTAILIVAGALGIFATAHEALAEYGDVVINNYSDAAGIR
                     PVVFPHWFHRMRFRCKVCHSDLGIKFAAGGNDINMLKIIDGQFCGACHNGSVAWAVEN
                     CELCHSGKPGTPTQVHESTLQRLKAPAAGK"
     gene            3360..4292
                     /locus_tag="VI319_00270"
     CDS             3360..4292
                     /locus_tag="VI319_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HEY6862309.1"
                     /translation="MKRTLIVLLATAAVAGAGPAAAQSKSAAGKAVFDKTCALCHATG
                     IAGAPKYGNARDWGPRVKTGLPALYRSALNGTARGMPGKGGNLSLSDAEVKAAVDYMV
                     AAVASEKAAPAPKAAPGEKTEAPKPAPVVVAPAAVVTASPNAFNRLLVPPARRNPAPA
                     EDGIHDPANPGTKALQPPLEAFAALPKSNAGNRVDWVGALGGDKISPRWDRDDDKAVP
                     PVMDLNIVREVKGSMPDVVYPHKQHTQWLDCSSCHPAIFIPQKGANQISMASILLGQK
                     CGVCHGKVAFPVSECRMCHSKKKDAGASVAGGRR"
     gene            4289..>4992
                     /locus_tag="VI319_00275"
     CDS             4289..>4992
                     /locus_tag="VI319_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862310.1"
                     /translation="MTRATLLAALLALALRAGAQVGSPSDAPVDVAALERRLHQVGVL
                     LETSSAAKQVVASGNPQALARREEARRIWADADKAYRAGMMLQASRLLPEASVRMFEA
                     VRMSAPEQVVAPKARSDFDARLESVRSLAAAQKRILKEKPGSPGAAEAAATVDALLAE
                     AEREEQAGRVEPARAALDRAYLVAKAAIGSMRGGDTLVRSLTFASAEEEYRYEIDRND
                     THRMLVKVVLAEKRDAG"
BASE COUNT          761 a         1819 c         1817 g          595 t
ORIGIN      
        1 ggtcgcgctc gcggtgcgcc agaagttcta tcaccgcaat ccctcggacg acgtgctcgc
       61 cggcgtgcgg cgcgaggtcg cgggcagcct ggtcgagcgc gtgctgctgc tgaaggaagc
      121 gaagcgccgc ggcctccggc ccgagcgcgc gcgcgtcgac gccgcgctcg ccgcgtacga
      181 ggagcgctac ggcgcgagcc cgcagtggcc gaaggtcaag gccgaggcgc tgccgaagct
      241 caccgcgcga ctcgaggagg acagcgtgct cgagcagctg gagcgcgcgg tgcgcgaggc
      301 gccgcagccc ggcgacaagg cgctgcgcgc gtattacctc cgccatcccg gcctcttcac
      361 cgagcccgag caggtgcgcc tctcggtgat cctgctgaaa gtcgatccgt cgtcgacgcg
      421 cgcggcctgg gacaaggccg aggccgaggc gcgcgacatc gtgaagcagc tcaaggccgg
      481 cgccgatttc gccgcgatcg cgcgcgagcg atcgggcgac gccgagtcgg cgaaggccgg
      541 cggcgacatg ggctatctgc accgcggcat gctgcccgag ggcctgcacg gcgaggtcga
      601 caagctgaag ccgggcgcga tctcgcagca gcccgtgcgc ctgctcgaag gcgtcgtcgt
      661 gctgcgcctg gaagaccgcc gggagcagcg cctgcgcggc ttcgacgagg tgcgcgcgcg
      721 cgccgtcgag ctggtgcggc gcgagcgctc ggacgcgaat tgggccgcgc tcgtctcgtc
      781 gctgcgcaag cgcgccgtga tcgaaatcga cggctcgcgc tatcccgagg cggccgccgc
      841 ggtggcggcc gcgcgcggcg gcgcccgcta ggtgcgaggc ggcgtgaagc gagcccggag
      901 aatcccgtgc gtgacggccg gcgaagcggc ggcgcgaggg ggctgatcgt gcgtctcgcg
      961 atggcggccc tgtcgtgcac gacgcccgcg ctcgccgcga cgggcgaccc gtgccccgag
     1021 ggcgacggcc tgagcctgtg ctcgccttcg ggcgcgcgcg cacgggaatc gtggatgagt
     1081 gcgcaggagt cggggagcct cgacgcgccc atcgaggtgc cggtcgagcc cgaggcggcg
     1141 cccgacgccc cgccgggaga tccgggcgcg ccgccgatgc gcggcctcga gtggcggctc
     1201 gcgcccgtgc gctggggcgg aaacgcctcc tacgagcttc ggcggctcgg cgtcgccggg
     1261 cacgccgcgc ggctgcagca ggtcgagacc gccaacctgc gcggcgcgag ctacgtgtgg
     1321 cagccctggt tcatgcaggt ctcgggcggc ctcgggatcg tcgcgggccg cgagcgctcg
     1381 cgcccggagg gcgacgactc caccgcggtc ggacgctcga attcgaacac ggtcaccggc
     1441 agcggcgcgc tctcggtgtt cccgtcgagc cgcttcccgt tccaggcgag cttcgacaag
     1501 accgacagcc gcgcgagcgg cgacatcacg agcaacgact tcaccaacac gcgctggggc
     1561 gcgcgccaga actaccgctc gggcgccggc gacctgcagt acacgggcag ctgggaccgc
     1621 ggcacgctcg agtcgccgtc gttcggccgg gacacggtcg acgtgctctc cgcgggcgtc
     1681 atccgctcgg cgggcccgca gacgctcgat ttctccggca gccgctcgcg caactggcgc
     1741 ggcgacaccg gcgacggctc gcgcatcgag cgcgtcgtgg cccgccacgg ctaccggccc
     1801 gatccgctcg cggcggtcga gacgctcgcg agctcgagca gctcgcgctt cgacctgacg
     1861 gcgaacggcg cgccgtcgga gaaccgcacg cgcttcgacc aggcgagcac cttcgcgacc
     1921 tggcgcgccg ggcagggctc gccgctctac ctctcgggcg gcggccgcgt gttcaggtcc
     1981 agcgtcgacg tcaacggcgc ggcgagcgac gcggacacgc tctcgggcaa cctcgccgcg
     2041 agctacgcgc tgtcgcggca cacgacgctg aacggcgcgg tgacggcgac ccgcgtcacg
     2101 accgacacgg cggcgcagtc gacgagcaac gtgatcacca ccgagagcgc cggcgcgact
     2161 tacgtgcccg acgcgacccc gctcggcggg ttcctctaca cgtggaacgc ggcggcgaac
     2221 gccgccaacc agaacggcgg cgtcgccgag gccgacgcga gcggcgcccg cagccgccag
     2281 aactacggcg ggcagctcgg ccacaacctg acgcgcagcg cgcccctgtc ggacgcgtcg
     2341 agcgtcacct atggcgccgg gcagagctac agcagcatct acgacacggt caccgagcgc
     2401 tcgcagacgc tgacgcacaa cgccagcgtc tcggtcagct cgtcgcccgg ctccgccgcg
     2461 accgcattcg cgagcctcta cggcgccgac gcgcgcaccc acggccacaa cgagaacacg
     2521 ttccagatcg tcaacctgca ggtcaacggg cagttccagc tgtcgcgctg gtcgctcgcg
     2581 aacgcgaacc tcacggtgca gggcgtgcgc caggagacgc cgggcgcgcc gccggcggga
     2641 ttcaaccgca gctcgggcgg caatctcacc taccagcacc tgcgcgcctt cgacctgccg
     2701 cggctgcgct acacggcgat ctacggcgcc aaccagatcc agtacagctc gcgcctcgag
     2761 ggcgacgtca acgcgccgcg cgagcgcgtc acgcgctcgt tcgagcagcg cctcgactgg
     2821 tcggtggggc ggctcgacct gcagctttcc gcgcgcacgg cgaccatcga cgggcagcgc
     2881 aacgagctcg tcttcttccg catcaaccgc cagttcggag cgttctagcg tggactcaaa
     2941 cggggcaact gaaatgtcga aacggcttcg cacggcgatc ctgatcgtcg ccggcgcgct
     3001 gggaattttc gccacggcgc acgaggcgct cgccgagtac ggcgatgtcg tcatcaacaa
     3061 ctactcggac gcggcgggca tccggccggt cgtgttcccg cactggttcc accgcatgcg
     3121 cttccgctgc aaggtctgcc acagcgacct cggcatcaag ttcgccgcgg gcggcaacga
     3181 catcaacatg ctgaagatca tcgacggcca gttctgcggt gcctgccaca acggcagcgt
     3241 cgcctgggcg gtcgagaact gcgagctgtg ccactcgggc aagccgggca cgccgacgca
     3301 ggtgcacgag agcacgctgc agcggctcaa ggcgcccgcg gcggggaagt gaggacgtca
     3361 tgaagcgcac gctcatcgtc ctcctcgcga ccgccgccgt ggccggcgcc ggacccgccg
     3421 ccgcgcaaag caagtccgcc gcggggaagg cggtcttcga caagacctgc gcgctgtgcc
     3481 acgcgaccgg catcgccggc gcgcccaagt acggcaacgc cagagactgg ggcccgcgcg
     3541 tgaagaccgg attgcccgcg ctctaccgca gcgccctgaa cggaaccgcc cgcggcatgc
     3601 ccggcaaagg cggcaacctc tcgctctcgg atgcggaagt gaaggcggcg gtggactaca
     3661 tggtcgcggc ggtcgcatcc gaaaaagcgg cgcccgcgcc gaaggcggcg ccgggcgaga
     3721 agaccgaggc gcccaagccc gcgccggtcg tcgtcgcgcc ggccgcggtc gtgacggcca
     3781 gcccgaacgc gttcaaccgc ctgctcgtgc cgcccgctcg acgcaacccc gcgcccgccg
     3841 aggacggcat ccacgatccg gcgaacccgg gcacgaaggc gctgcagccg ccgctcgagg
     3901 cgttcgcggc gctgccgaag agcaacgccg gcaaccgcgt cgactgggtg ggcgcgctcg
     3961 gcggggacaa gatcagcccg cgctgggacc gcgacgacga caaggcggtg ccgccggtca
     4021 tggatctcaa catcgtgcgc gaggtgaagg gctcgatgcc cgacgtcgtg tatccgcaca
     4081 agcagcacac gcagtggctc gactgctcga gctgccaccc ggcgatcttc attccgcaga
     4141 aaggcgcgaa ccagatcagc atggcctcga tcctgctcgg gcagaagtgc ggcgtgtgcc
     4201 acggcaaggt cgcgttcccc gtctccgagt gccgcatgtg ccactcgaag aagaaagacg
     4261 ccggggcgag cgtcgcggga gggcgcaggt gacgcgcgct acgctgctcg cggcgctgct
     4321 cgcgctcgcg ctccgcgccg gcgcgcaggt cggctcgccg agcgacgcgc cggtcgacgt
     4381 cgcggcgctc gagcgccgcc tgcatcaggt cggcgtgctg ctcgagacct cgagcgccgc
     4441 gaagcaggtc gtcgcgagcg gcaacccgca ggcgctcgcg cgccgcgagg aggcgcgccg
     4501 catctgggcc gacgccgaca aggcgtaccg cgccggcatg atgctgcagg cgtcgcgcct
     4561 gctgcccgag gcgagcgtgc gcatgttcga ggcggtgcgc atgtcggcgc ccgagcaggt
     4621 cgtcgccccg aaggcgcgca gcgacttcga tgcgcgcctg gaaagcgtgc gctctctcgc
     4681 cgcggcgcag aagcggatcc tcaaggagaa gcccgggtcg cccggcgcgg ccgaggcggc
     4741 ggcgacggtc gacgcgctgc tcgccgaggc cgagcgcgag gagcaggccg ggcgcgtcga
     4801 gccggcccgc gccgcgctcg accgcgccta cctggtcgcg aaggccgcga tcggctcgat
     4861 gcgcggcggc gacacgctgg tgcgctcgct cacgttcgcg agcgcggagg aggaataccg
     4921 ctacgagatc gaccgcaacg acacgcaccg gatgctggtg aaggtcgtgc tcgcggagaa
     4981 gcgcgacgcg gg
//
LOCUS       DATDMW010000008         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2242231, whole genome shotgun sequence.
ACCESSION   DATDMW010000008 DATDMW010000000
VERSION     DATDMW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242231"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="rfbA"
                     /locus_tag="VI319_00280"
     CDS             complement(<1..778)
                     /gene="rfbA"
                     /locus_tag="VI319_00280"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEY6862311.1"
                     /translation="MKGIILAGGSGTRLYPVTQVVSKQLLPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPDDTPRFEKLLGDGSRWGLNFAYAVQAAPKGIAEAFLIAERFIGNDCC
                     GLVLGDNIFYGHDLAAEIERAAQKIRGATVFAYRVNDPQRYGVVEFDGAGRAISIEEK
                     PERPKSRFAVTGLYFYDNDVIGIAKSLKPSERGELEITDVNRKYLARGDLDVVPLGRG
                     HAWLDTGTHETLLEASHFIETLERRQGLKICCPEEIAWRLG"
     gene            complement(775..1845)
                     /gene="rfbB"
                     /locus_tag="VI319_00285"
     CDS             complement(775..1845)
                     /gene="rfbB"
                     /locus_tag="VI319_00285"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY6862312.1"
                     /translation="MTILVTGGAGFIGSNFVLGWLASSDEPVVNLDKLTYAGNLENLS
                     AAQVNASHLFVHGDIGDRTLLKQLLDKQAPRAIVHFAAESHVDRSIHGPREFIDTNVL
                     GTFALLEEARDFLRKLAPAAQEAFRFVHVSTDEVYGSLGPADPPFRETTAYAPNSPYA
                     ASKAASDHLVRAYHATYGLPAIITNCSNNYGPHQFPEKLIPLMILNAISGKPLPVYGD
                     GGNVRDWLFVQDHCAALRLVLARGRPGETYNIGGSSERSNIEVVKAVCALLDEAKPHR
                     KHNHADLITFVADRPGHDRRYAIDASKIGRELGWRPAESFESGLRKTVAWYLDNPEWV
                     AHVASGAYRGWIEKNYGKRGSA"
     gene            complement(1842..>2147)
                     /locus_tag="VI319_00290"
     CDS             complement(1842..>2147)
                     /locus_tag="VI319_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6862313.1"
                     /translation="LTAEAVQRLAGDLMHDSAETLERYLDKQNRYTTLQAETLRAAGA
                     RASLAHLALSPLFRFLKFYVLRLGFLDGAPGLVHIAIGCMNSFNKYAKLMALERAGR"
BASE COUNT          325 a          698 c          746 g          378 t
ORIGIN      
        1 ggccgagccg ccaggcgatc tcctccggac agcagatctt cagtccctgg cgccgctcca
       61 gggtctcgat gaagtgactc gcctcgagca gcgtctcgtg cgttcctgtg tcgagccagg
      121 cgtgtccacg gccgagcgga acgacgtcga ggtcgccgcg tgcgaggtac ttccggttca
      181 cgtcggtgat ctcgagctct ccacgctccg acggcttcag cgacttggcg atcccgatca
      241 cgtcgttgtc gtagaagtat agaccggtga ccgcgaagcg cgacttgggt cgctcgggct
      301 tctcttcgat gctgatggcc cgaccggcgc cgtcgaactc gacgacccca tagcgctgtg
      361 gatcgttcac gcggtaggca aacaccgtag cgccccggat tttctgcgcg gcgcgctcga
      421 tctcggccgc caggtcgtgc ccatagaaga tgttgtcccc caggacgagc ccgcagcagt
      481 cgtttccgat gaagcgctcc gcgatcagaa aggcctcggc gatccctttt ggggcggcct
      541 gcacggcgta ggcgaaattc aatccccacc gggatccgtc acccagcagc ttctcgaatc
      601 tcggcgtatc gtcgggcgtc gagatgatca ggatgtcgcg gatccccgcc agcatcagcg
      661 tcgacagcgg atagtagatc atcggcttgt cgtacaccgg cagcagctgc ttggagacga
      721 cctgcgtcac ggggtagagc cgcgtcccgg atccgccggc gaggatgatg cccttcacgc
      781 gctcccccgc ttgccgtaat tcttctcgat ccagccgcga tatgcgccgc tcgcgacgtg
      841 cgccacccac tccgggttgt cgagatacca cgcgacagtc ttgcgcagcc ccgactcgaa
      901 gctctccgcc ggccgccagc cgagctcgcg gccgatcttc gacgcgtcga tcgcatagcg
      961 ccggtcgtgc cccgggcggt cggcgacgaa cgtgatcagg tcggcatggt tgtgcttgcg
     1021 gtgcggcttg gcctcgtcga gcagggcgca gaccgctttc accacctcga tgttgctgcg
     1081 ctcgctcgac ccgccgatat tgtaggtctc gcccgggcgg ccgcgcgcga gcaccaggcg
     1141 cagcgccgcg cagtggtcct gcacgaacag ccagtcgcgc acgttgccgc cgtcgccgta
     1201 gaccggcagc ggcttccccg agatcgcgtt caggatcatg agcgggatga gtttttcggg
     1261 gaactggtgc ggcccgtagt tgttcgagca gttggtgatg atcgccggca agccgtaggt
     1321 tgcgtggtag gcgcgcacca ggtggtcgga cgcggccttg gaggcggcgt acgggctgtt
     1381 cggcgcgtac gcggtggtct cgcggaacgg cggatcggcc gggccgagcg agccgtagac
     1441 ttcgtcggtc gagacgtgca cgaagcggaa cgcttcctgc gccgcaggcg cgagcttgcg
     1501 caggaagtcg cgcgcttcct cgagcagcgc gaaggtgccg agcacgttgg tgtcgatgaa
     1561 ctcgcgcggg ccgtggatcg agcggtcgac gtggctctcg gcggcgaaat gcacgatggc
     1621 gcgcggcgcc tgcttgtcga gcagctgctt gagcagcgtc cggtcgccga tgtcgccgtg
     1681 cacgaacaag tgcgacgcgt tcacctgcgc ggcgctcagg ttctcgaggt tgcccgcgta
     1741 ggtgagcttg tccaggttga cgaccggctc gtcgctgctc gcgagccacc cgagcacgaa
     1801 gttcgagccg atgaagcccg cgccgccggt gacgaggatg gtcatcggcc ggcgcgctcc
     1861 agcgccatga gcttcgcgta cttgttgaag ctgttcatgc agccgatggc gatgtgcacg
     1921 aggccgggcg cgccgtcgag gaagccgagc cgcagcacgt agaacttgag gaaccggaac
     1981 agcggcgaga gcgcgaggtg cgcgagcgag gcgcgggcgc cggccgcgcg cagcgtctcg
     2041 gcctgcagcg tcgtgtagcg gttctgcttg tcgaggtagc gctcgagcgt ctcggccgag
     2101 tcgtgcatca ggtcgcccgc gagccgctgc acggcttcgg cggtcag
//
LOCUS       DATDMW010000009         2693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1993129, whole genome shotgun sequence.
ACCESSION   DATDMW010000009 DATDMW010000000
VERSION     DATDMW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993129"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VI319_00295"
     CDS             <1..391
                     /locus_tag="VI319_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY6862314.1"
                     /translation="AVDAKVRTGRAAVKWQRQGSPRDFVTFEYVMLEGVNDADEHARE
                     LIELAGKVRCKFNLIPFNPFPGSDFRRSDAARIGRFAAILQRAGLTVTTRKTRGDDIA
                     AACGQLAGDVADRTRRRDAIPLREVRA"
     gene            388..1170
                     /gene="pilW"
                     /locus_tag="VI319_00300"
     CDS             388..1170
                     /gene="pilW"
                     /locus_tag="VI319_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY6862315.1"
                     /translation="MKRARLLGLVALALLGACARQPEGPTTADSGMIGEVGDARNRAR
                     VHSELAAAYYDSGNMAVALDELRRATAADSSYAPAYNMLGRVYMELKENRLADDNFGR
                     ALRLAPNDPDINHSYGWFLCQTGQEKESIRYFLQAIRNPLYAAPWRSYSAAGLCSLRE
                     DNVKDADEFFQRALRLEPDEPTATLKLAGIRYRQGRMDEARRLVNRYNKVQTPSAESL
                     WLGVRVERRVGDRLAERSYAEQLRRRFPGSPELRSLQQGLYD"
     gene            1163..2179
                     /locus_tag="VI319_00305"
     CDS             1163..2179
                     /locus_tag="VI319_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEY6862316.1"
                     /translation="MTESALKVGAELQRAREARGLALSEVAQQLKFAPRQLEALEQDR
                     FDLLPGGTFARGMVRNYARLLKIDPEPLVRQVGERFEAPDANTLAARYSQPVPFSDNA
                     RRSTFMYLGLSLGVLAIGGGVAWQWYHERHRTSAPLARADAPRPATRLAAAPSASQKA
                     DRKSDPSPKAKAVEAPPKPKLADAAPKAKAPESAPKAPASAPQAPESPPKAAEPAPKL
                     AQAAVARAAAAPDAAAKGVGQNPAAEKAAETKPVQIANVAAGVHRLVIRCDAEAWIEV
                     RTQDDRALISSLNPAGSERSVRARGPLNLVIGNASHVLVLHNDRPLDLQPYVKSDIAR
                     FTLP"
     gene            2176..>2693
                     /locus_tag="VI319_00310"
     CDS             2176..>2693
                     /locus_tag="VI319_00310"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY6862317.1"
                     /translation="MTRRVSRQVRVGSGPSAVLIGGGAPVVVQSMTNTDTADVQATAR
                     QVEELARAGSEIVRITVNTPEAAAAVPRIRERLDAIGCRVPLVGDFHFNGHKLLTQFP
                     ECAGSLAKYRINPGNVGRGAKRDEQFAQMIDCALRHEKPVRIGVNWGSLDAELLARMM
                     DENARRSEPEPAV"
BASE COUNT          423 a          980 c          944 g          346 t
ORIGIN      
        1 ggcggttgac gccaaggtcc ggaccggccg tgctgccgtc aagtggcagc ggcagggttc
       61 gccgcgcgac ttcgtgacct tcgagtacgt gatgctcgaa ggcgtgaacg acgccgacga
      121 gcacgcgcgc gagctgatcg agctcgccgg caaggtgcgc tgcaagttca acctgatccc
      181 gttcaatccg tttccgggtt cggacttccg ccgctccgac gccgcgcgca tcggccggtt
      241 cgccgcgatc ctgcagcgcg cggggctcac ggtgacgacg cgcaagaccc gtggcgacga
      301 catcgccgcc gcgtgcggcc agctcgccgg cgatgtcgcc gatcgtacgc ggcgccgcga
      361 cgcaataccg ctcagggaag tgcgcgcttg aagcgggcgc ggctcctcgg cctcgtcgcg
      421 ctggcgctgc tcggcgcgtg cgcgaggcag ccggaagggc cgaccaccgc cgacagcggc
      481 atgatcggcg aagtgggcga tgcgcggaac cgcgcgcgcg tgcacagcga gctcgcggcc
      541 gcctactacg acagcggcaa catggccgtc gcgctcgacg agctgcgccg cgccacggcg
      601 gccgactcga gctacgcgcc cgcgtacaac atgctcggcc gcgtgtacat ggagctcaag
      661 gagaaccggc tcgccgacga caatttcggg cgcgcgctga ggctcgcacc gaacgatccg
      721 gacatcaacc acagctacgg ctggttcctc tgccagacgg ggcaggagaa ggaatcgatc
      781 cgctacttcc tgcaggcgat ccgcaatccg ctgtatgcgg cgccctggcg ctcctactcg
      841 gcggccggcc tgtgcagcct gcgcgaggac aacgtcaagg acgccgacga gttcttccag
      901 cgcgcgctgc gcctcgagcc cgacgagccg accgcgacgc tgaagctcgc cggcatccgc
      961 taccgccagg gccgcatgga cgaggcgcgc cgcctcgtca accgctacaa caaggtgcag
     1021 accccgagcg cggaatcgct gtggctcggc gtgcgggtcg agcgccgggt cggcgatcgg
     1081 ctcgccgagc gcagctatgc cgagcaactg cgtcggcgct tccccggatc gccggagctg
     1141 cgctcgctgc agcagggact ctatgactga gagcgcgctc aaggtgggcg ccgagctgca
     1201 gcgcgcgcgg gaggcgcgcg gcctcgcgct ttccgaggtg gcccagcagc tgaagttcgc
     1261 gccgcgtcag ctcgaagcgc tggagcagga ccgcttcgac ctgctgcccg gcggcacgtt
     1321 tgcccgcggc atggtgcgca actacgcccg gctgctgaag atcgacccgg agccgctggt
     1381 gcgccaggtc ggcgagcgct tcgaggcgcc cgatgccaat acgctcgccg cgcgctacag
     1441 ccagccggtg ccgttctcgg acaatgcccg ccgctcgacg ttcatgtacc tcgggctctc
     1501 gctcggcgtg ctggcgatcg gcggcggggt cgcatggcag tggtaccacg agcgccaccg
     1561 gacgagcgcg ccgctcgcgc gcgccgatgc gccgcggccg gccacgcgcc tcgcggctgc
     1621 gccgtccgcc tcgcagaaag ccgacaggaa gagcgatccg tcgccgaagg cgaaggcggt
     1681 cgaagcgccg cccaagccga agctggcaga cgccgcgccc aaggcgaagg cgcctgaatc
     1741 cgccccaaag gcgcccgcgt ccgcgccgca ggcacccgag tccccgccta aggcggccga
     1801 gcccgctccg aagctcgcgc aggctgcggt cgcgcgggcc gccgcggcgc cggatgccgc
     1861 ggcgaaaggc gtcggccaga accccgccgc cgaaaaggcg gccgagacga agccggtcca
     1921 gatcgccaat gtcgccgccg gcgtgcatcg gctggtcatc cgctgcgacg cggaagcgtg
     1981 gatcgaagtg cgaacgcagg acgaccgggc gctcatctcc tcgctcaacc ctgcgggctc
     2041 ggaacgctcg gtgcgcgcgc gcggcccgct caacctggtg atcggcaacg caagccatgt
     2101 gctcgtcctg cacaacgacc ggccgctcga cctgcagcct tacgtcaagt ccgacatcgc
     2161 ccgcttcacc ctgccatgac gagacgcgtt tcgcgccagg tccgcgtcgg cagcgggccc
     2221 tccgccgtgc tcatcggcgg cggcgcgccg gtcgtggtgc agtcgatgac caataccgac
     2281 acggcggacg tgcaggccac cgcgcgccag gtggaagagc tcgcacgcgc gggctcggag
     2341 atcgtgcgca tcacggtgaa tacgcccgaa gccgccgccg ccgtgccgcg catccgcgag
     2401 cggctcgacg cgatcggctg ccgcgtgccg ctggtcggcg acttccactt caacgggcac
     2461 aagctgctca cgcagtttcc cgagtgcgcg ggctcgctcg ccaagtaccg gatcaacccg
     2521 ggcaacgtcg gcaggggcgc gaagcgcgac gagcagttcg cgcagatgat cgactgcgcg
     2581 ctgcgccacg agaagccggt gcgcatcggc gtgaactggg gcagcctgga tgcggagctg
     2641 ctcgcccgca tgatggatga aaacgcgcgc cgttccgaac ccgagcccgc cgt
//
LOCUS       DATDMW010000010         3388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1371014, whole genome shotgun sequence.
ACCESSION   DATDMW010000010 DATDMW010000000
VERSION     DATDMW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371014"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VI319_00315"
     CDS             complement(<1..1845)
                     /locus_tag="VI319_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6862318.1"
                     /translation="MRTAIVSTYPPRACGIGTFSADLRATLAGTRTVTRADLVAVVHE
                     PSSPQRRGLLATIAQAVRGDYVRTARMLGRLDVDVVLLQHEYGIFGGRDGEYVLSFAQ
                     ELAQPLVVTLHTVLSEPTPHQEEVLTALCAEAELVIVMTDTALRFLVESGTCSEEKVR
                     VVPHGAPARIAERASREPARQFTSSGVKDPFRLSTFGLISPGKGLETVLDALPAMIER
                     HPEIVYTIAGRTHPDVSRREGERYRLTLERRAHELGLSDQVEFDDRFLSIDELSDLLA
                     NTDVFLTPYGNREQIASGALTFALAAGCAVVSTPYWYAQDMLASGAGILVPFRDSEAL
                     ADAVCSYVERPDMLRAARTEAQRIGSTLAWPAVAEATARVLHEACELAPLRRPAGVGD
                     LHLPSLRTDHLLTLADDVGIVQHAHGIIPNRSSGYCVDDVARFAVVSLALAGRGDEQV
                     WTSNLYRALAFLQAAADEDGGMRNFMGYDRRWLDEPHVGDHVGRSIWALGDILATAWI
                     PAVVRPAADLLDRLVGMLGADVSLRTAAYAVLGLSHVDADRLEPAAHRRLELLVEQLA
                     EAYEGTCSGDWCWFEDELTYDNARLSQALLTGAYALGRTELAEAGLESL"
     gene            complement(1849..1998)
                     /locus_tag="VI319_00320"
     CDS             complement(1849..1998)
                     /locus_tag="VI319_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862319.1"
                     /translation="MKKALKGILDRLRTATNGRLRKPTSTRDGLQVPQRLQALSASPR
                     MRPVR"
     gene            complement(2322..3242)
                     /locus_tag="VI319_00325"
     CDS             complement(2322..3242)
                     /locus_tag="VI319_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY6862320.1"
                     /translation="MIAWIVPPIVVVFVLFTLWRTVRIIPQARAGVVERFGRYSRTLA
                     PGLTIVVPFVDRLRPLIDQREQVVSFAPQPVITEDNLGVNIDTVIYFQVTDAKAASYE
                     IADYIQAIEQLTVTTLRNVIGGIALERTLTSRDHINTQLRSVLDDATGKWGIRVNRVE
                     LKAIDPPASIQESMEKQMRADREKRATVLNAEGVRQSEILEAEGAKQSAILRAEGDRE
                     SQILRAEGQAKAIETVFSAIHAGDADPKLLAYQYLQMLPQIAQGDSNKVWLIPTEFSH
                     ALDRFQEQFIPGANGNTPVPPVAKNVAGKQ"
BASE COUNT          544 a         1115 c         1134 g          595 t
ORIGIN      
        1 cagcgattcg agtccggcct cggcgagctc cgtccggccg agtgcgtacg cgccagtgag
       61 gagggcctgc gacagccgcg cgttgtcgta ggtgagctcg tcctcgaacc agcaccaatc
      121 ccccgagcac gtgccctcgt aggcttcggc gagttgctcg acgagcagct cgagccgccg
      181 atgcgcggcg ggctcaagcc ggtcggcgtc gacgtgggag aggccgagca cggcataggc
      241 cgccgtgcgc agggaaacgt cagcaccgag catgccgacg agccggtcga gcaggtcggc
      301 cgccggccgc acaacggcgg ggatccaggc agtggcgagg atgtccccga gcgcccagat
      361 cgagcgtccc acgtggtcac cgacatgcgg ctcgtcgagc cagcgacggt cgtaacccat
      421 gaagttgcgc attccgccgt cctcgtccgc agccgcttgg aggaatgcga gcgcgcgata
      481 gagattcgag gtccagacct gctcatcgcc gcgccctgca agtgcgagcg aaacaaccgc
      541 gaagcgggca acatcgtcga cgcaataacc gctgctgcgg ttgggaatga ttccgtgcgc
      601 gtgctgcacg atgccgacat cgtcggcgag tgtgagcaga tggtcggtgc gcagactcgg
      661 caggtgcagg tcgccgacgc cagcgggccg tcgcagcggc gccagctcgc aggcttcgtg
      721 caagacccga gccgtggcct ctgcgacagc cggccaagcg agcgtcgagc cgatccgctg
      781 tgcttctgtg cgcgccgccc tcagcatgtc cggccgctcg acgtagctac agaccgcgtc
      841 ggcaagcgct tccgaatcgc ggaacggcac caatatcccg gcccccgagg cgagcatgtc
      901 ctgtgcatac cagtacgggg tggagacgac cgcgcaaccg gcagcgagtg cgaacgtgag
      961 ggcgccggag gcgatctgct cccggtttcc gtacggcgtc aggaagacgt cggtgttggc
     1021 gagcaggtct gagagctcgt cgatcgacag gaaccgatcg tcgaactcga cctggtcgct
     1081 gagcccgagc tcgtgcgccc gccgttccag cgtcagccgg taccgctccc cttcccgacg
     1141 cgagacatcc ggatgggtgc gcccggcgat cgtgtagacg atctcggggt gccgttcgat
     1201 catggccggt agcgcgtcga gcactgtctc caatcccttg ccgggcgaga tcaggccgaa
     1261 cgtcgaaagc cggaagggat ccttgacgcc cgatgacgtg aactgccgtg ccggctcacg
     1321 cgacgcccgc tcggcgatgc gcgccggcgc tccgtgcggc acgacccgga ctttctcctc
     1381 cgagcaagtg cccgactcga ccaggaaccg aagcgcggtg tccgtcatca cgattacgag
     1441 ctccgcctct gcgcatagcg cggtcagcac ttcctcctgg tggggcgtgg gctccgacag
     1501 cacggtgtgc agcgtcacca cgagcggctg cgccagttct tgggcgaacg acagcacgta
     1561 ctccccgtcg cggccgccga agatcccgta ctcgtgctgg agcaagacga cgtcgacatc
     1621 gagccgcccg agcatccgag cagtccgcac gtagtcgccg cgcaccgcct gcgcgatcgt
     1681 cgcgagcaga ccccgccgct gtgggctcga cggttcgtgc acgaccgcga cgagatcggc
     1741 gcgcgtaacc gttcgcgtgc cggccagtgt cgctcggagg tcggctgaga aagttcctat
     1801 cccgcaggca cgcggtgggt aggtggaaac gatcgctgtc cgcatgcgct accgcaccgg
     1861 tcgcattcgc ggggacgcgc tcagcgcttg caatcgctgc ggcacctgca gaccgtcacg
     1921 cgtcgacgtc ggtttgcgga gtctcccgtt ggttgccgtg cgcagccggt cgagtatccc
     1981 tttcagcgcc ttcttcatga ggttgctcct ttagttgact ccttcgcaac agcagccggt
     2041 aggcggacaa ccgtcgacgg tgccggtgcg ctctcggatc tgtacgccgc ataggcctcg
     2101 agggcctcga gatcgtggcg gcgacgcccc gggaagaagc gccccaggaa ctcagaccag
     2161 ctgagtccgg ctgcagcgcg ctcgccgcgc gtctcgatct cgggggcgtc gttggcgctt
     2221 tgcttgcctc cttcgtcttc ccagcgttga agaggctcac actcgctgcg gggcgccgac
     2281 acgaaccccc gctttgcctg gacgttcgag ctgaacacgg cctactgctt tcccgcgacg
     2341 ttcttggcga ccggcgggac cggcgtgttg ccgttcgcgc ccgggatgaa ctgctcctgg
     2401 aatcgatcga gcgcgtgtga gaactcggtc gggatcagcc agaccttgtt cgagtcgccc
     2461 tgggcgattt ggggcagcat ctgcaggtac tggtaggcga gtagcttggg gtctgcgtcg
     2521 ccggcatgga tcgcgctgaa gaccgtctcg atcgcctttg cctgcccttc ggcgcgcagg
     2581 atctgcgatt cccggtcccc ttccgcacgc aggatcgcgc tctgtttggc accttcggcc
     2641 tcgaggatct ccgactgcct gacgccttcg gcgttgagga ccgtggcgcg tttctcgcga
     2701 tctgcgcgca tttgcttttc catcgattcc tggatcgacg ctggcgggtc gatcgctttc
     2761 agttcgaccc ggttgacccg gatgccccac ttgccagtcg cgtcgtcgag aacgctgcgt
     2821 agctgggtgt tgatgtgatc gcgcgaggtg agtgtcctct cgagcgcgat cccgccgatc
     2881 acgttccgca aggttgttac cgtcagctgt tcgatcgcct ggatgtagtc ggcgatctcg
     2941 tagctcgcgg ccttggcgtc ggtgacctgg aaatagataa cggtgtcgat gttgaccccc
     3001 aggttgtcct cggttatcac cggctgcggt gcgaacgaga ccacttgttc gcgctggtcg
     3061 atgagcggcc gcagccggtc gacgaagggc acgacgatcg tcagccccgg cgcaagcgtg
     3121 cgcgagtagc ggccgaagcg ctcgacgaca ccggcccgcg cctgcggaat gatccgaacc
     3181 gtccgccaca gggtgaagag gacaaagacg acgacgatag gggggacgat ccaggcgatc
     3241 atttgacgtt cagctcctat ctgcaaacga gagcggtagc gccctcgatc agtgcgattt
     3301 cgaccggggg atcaacggct gcgccttgtt gcaggtcttg cagacgtggc ccgcgatcac
     3361 acggccgctg acctgtggcg tgaacgcg
//
LOCUS       DATDMW010000011         4794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_126230, whole genome shotgun sequence.
ACCESSION   DATDMW010000011 DATDMW010000000
VERSION     DATDMW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126230"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="gatB"
                     /locus_tag="VI319_00330"
     CDS             <1..616
                     /gene="gatB"
                     /locus_tag="VI319_00330"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEY6862321.1"
                     /translation="PLVIADSWIDEVEAQLAKIGTPESKRAQFTLEYGSSLTPYDTVL
                     LTESREKSEFFERMVARLGASSAKLCANWVSGELAAALKRENLDVSESRITADQLATL
                     LTRVADNTLSGKMAKEVFDAMWAGEGAADAIIEKRGLKQITDAGEIERIVDEVLAANA
                     RQVEDYRAGKEKAFNSLVGQAMKATRGKGNPATINELLRKKLGR"
     gene            complement(627..1265)
                     /locus_tag="VI319_00335"
     CDS             complement(627..1265)
                     /locus_tag="VI319_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY6862322.1"
                     /translation="MLSRAIVVLAALALAPSSWAQSSGHLYKCVDARGKVYYTQLPPP
                     ECAGRASTELDKSGSFVVKQNAAPPTAEERAKVEAQKEADRKRKAEEEERLKEERRLS
                     AALLNTYSSVKDIDDARARALKDNAVAVQDAEKHYADALKRQDALNKEKASYANKALP
                     RKLELDLETAGVDIQNWQQAIDARRKEASAINARYDEDKRRYVELTRATPKR"
     gene            complement(1258..1908)
                     /gene="pyrE"
                     /locus_tag="VI319_00340"
     CDS             complement(1258..1908)
                     /gene="pyrE"
                     /locus_tag="VI319_00340"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY6862323.1"
                     /translation="MSDYREDFIAFCVESGALKFGKFTTKSGRVSPYFFNIGAFASGA
                     ALERLAQFYAKAIAASSVRFDMIFGPAYKGIALAAATAIALARAGRDVPYAFNRKEAK
                     DHGEGGDTIGAPLAGRVLIVDDVITAGTAVRESLALIRAHGAAPAALVIALDRMERGE
                     GEASAIRELEQRHGVPVIAIASLDDLVRWLEGRAGMQQHLKAIDEYRQLYGAANHA"
     gene            1936..2727
                     /locus_tag="VI319_00345"
     CDS             1936..2727
                     /locus_tag="VI319_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY6862324.1"
                     /translation="MLRIITLNLNGIRSALRKGFPSWLARQRADIVCLQEVKAQEADL
                     ADLALVPRGFHGHYHCAARRGYSGVALYSRMKPDKVICGFGSREFDPEGRFLRADFGR
                     LSVVSVYLPSGSSGEERQQAKFRFLDEFLPVLERLRRSRREFVLCGDWNIAHKEIDLR
                     NWRSNRKNSGFLPEEREWLTGVFERKGWVDVFRRLDPRPGQYTWWSTRGRAWAKNVGW
                     RIDYQVATAGIAAKATKAAIYKARRFSDHAPLIVDYDWSLEGVGG"
     gene            complement(2694..3041)
                     /locus_tag="VI319_00350"
     CDS             complement(2694..3041)
                     /locus_tag="VI319_00350"
                     /inference="COORDINATES: protein motif:HMM:NF025363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HEY6862325.1"
                     /translation="MTRLDELALKRERLVARAAEQRAAIATSLAGCRSVLSIADRAFV
                     WGQWARRHPALLAISTAVLVAARPRLGLRWVVRALSVWRTGRFALDLVKTFAAMRAAR
                     APAQPPTPSSDQS"
     gene            complement(3025..3423)
                     /locus_tag="VI319_00355"
     CDS             complement(3025..3423)
                     /locus_tag="VI319_00355"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY6862326.1"
                     /translation="MSDEAGRAVGVLDSLRSLAATLIAVVQTRLELVATDIEEERAWI
                     ARMAILAALAGFCLALAINLVVLLLVVLFWDANRLLAIGVLAGVFAAFAAGLALAARS
                     TAARRPRLFSATLAELRKDREQAQPRDPSR"
     gene            complement(3428..3739)
                     /locus_tag="VI319_00360"
     CDS             complement(3428..3739)
                     /locus_tag="VI319_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEY6862327.1"
                     /translation="MQSTNDIANDKLATDLRRIIGDAEELLRATANQAGEKVAAARAR
                     VQDSLDSAKLKLELLGEEAADQARAAVHATDDYVHDHPWQSVGIAALVGLVLGMMVSR
                     K"
     gene            3859..>4794
                     /locus_tag="VI319_00365"
     CDS             3859..>4794
                     /locus_tag="VI319_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6862328.1"
                     /translation="MPSDAPPTGKTSWARALLNRRMLICVFTGFSSGLPLYVLISLVP
                     AWLKTEGLSLAVIGGFALIQFPYTWKFLWAPLADRYSLPWLGRRRSWMLLAQLGVLAC
                     IWALGTLSPARDLQGVIAITVAVALLGATLDIALDAYRRELLREEELGLGNAVHVNAY
                     RIAGLVPGSLALILADHYAWDFVFAVTAAFMLPGVVMALVVAEPATPAGAPRTLREAV
                     VEPFHEFMTRHGWREALFVLAFVFLYKLGDAMCTALATPFYLDMGFSKSEIGLVAKHA
                     GLWPSVIGGLLGGLWMVRLGINRALWLFGVVQLVSI"
BASE COUNT          703 a         1625 c         1675 g          791 t
ORIGIN      
        1 gcccctcgtc atagcagact cttggatcga cgaagtcgaa gcccaactcg caaagatcgg
       61 tactccagaa tccaagcgag ctcaattcac actggagtat ggatccagtc tgacgccata
      121 cgacacggtt ctccttacgg aatcacgaga gaagtcggaa ttctttgaaa gaatggtcgc
      181 tcgtttagga gctagctcgg caaagttgtg cgcgaactgg gtcagcggtg aactggcggc
      241 tgcactgaag cgtgagaacc tcgatgtctc tgaatcgcgc attacagctg atcagcttgc
      301 gaccttattg acccgcgtgg ccgacaacac gctgtcgggc aagatggcga aggaggtctt
      361 cgacgcgatg tgggccggcg agggcgcggc cgacgccatc atcgaaaagc gcggcctgaa
      421 gcagatcacc gacgccggcg agatcgagcg catcgtggac gaggtgctcg ccgcgaacgc
      481 caggcaggtc gaggactacc gcgccgggaa ggaaaaggcg ttcaactcgc tggtggggca
      541 ggcgatgaag gcgacgcgcg gcaagggcaa ccccgcgacg atcaacgagc tgctgcggaa
      601 gaagctcggc cgctgaggcc ggcgcgctag cgtttcggcg tggcgcgcgt gagctcgacg
      661 tagcgtcgct tgtcctcgtc gtacctggcg ttgatcgcgg acgcttcctt gcggcgcgca
      721 tcgatcgcct gctgccagtt ctggatgtcg acgcccgcgg tctccaggtc gagctcgagc
      781 tttctcggca gggccttgtt cgcgtaggag gccttctcct tgttgagggc gtcctggcgc
      841 ttcagcgcgt cggcgtagtg cttctccgcg tcctggaccg ccacggcgtt gtccttcagc
      901 gcccgggcgc gcgcgtcgtc gatgtccttg acgctggaat aggtgttgag cagcgcggcg
      961 ctcaggcggc gctcttcctt caggcgctcc tcctcctcgg ctttgcgctt gcggtcggcc
     1021 tctttctgcg cctcgacctt cgcgcgctcc tcggcagtgg gcggggcggc attctgcttg
     1081 acgacgaacg agccggactt gtcgagctcg gtcgaagcgc ggccggcgca ttccggcggc
     1141 ggcagctgcg tgtagtacac cttgccgcgg gcgtccacgc acttgtacaa atgtcccgac
     1201 gactgcgccc acgacgacgg cgcgagcgcg agcgcggcga ggacaacgat tgcacggcta
     1261 agcatgattg gccgctccat agagttggcg atactcgtcg atcgccttga gatgttgctg
     1321 catgccggcc cggccctcga gccagcgcac gaggtcgtcg aggctcgcga tcgcgatcac
     1381 cgggacgccg tggcgctgct cgagctcgcg gatcgccgag gcctcgcctt cgccgcgctc
     1441 catgcgatcg agcgcgatga cgagcgccgc cggcgcggcg ccgtgcgcgc ggatgagcgc
     1501 gagcgactcg cgcaccgcgg tcccggccgt gatcacgtcg tccacgatca gcactcggcc
     1561 cgcgagcggc gcgccgatgg tatcgccgcc ttcgccgtgg tccttggctt ccttgcgatt
     1621 gaacgcgtag ggcacatcgc ggcccgcgcg tgcgagcgcg atcgcggtgg ccgcggcgag
     1681 ggcgatgccc ttgtaggccg ggccgaagat catgtcgaag cggacgctcg aggcggcgat
     1741 cgccttcgcg tagaattgcg ccagccgctc gagcgcggcg ccgctcgcaa aggcgccgat
     1801 gttgaagaaa tacggcgaga cgcgacccga cttcgtcgtg aactttccga acttcaacgc
     1861 gccgctctcg acgcagaagg cgatgaagtc ttcgcggtaa tccgacacga tccgtcccga
     1921 aaatgaatcg gcccgatgtt acgcataatc acgctcaacc tcaacggaat taggtccgcg
     1981 ctcaggaaag gattcccgtc ctggctggcg cggcagcggg cggatatcgt ctgccttcag
     2041 gaggtgaagg cccaggaggc cgacctcgcc gacctggcgc tcgtcccccg cggcttccac
     2101 ggccattacc attgcgcggc ccggcgcggc tacagcggcg tcgcgctcta ctcgcgcatg
     2161 aagcccgaca aggtgatctg cggcttcggc agccgcgagt tcgatcccga gggccgattc
     2221 ctgcgcgcgg atttcgggcg gctctcggtc gtgtcggtct acctgccgtc gggctcgagc
     2281 ggggaggagc gccagcaggc caagttccgc ttcctcgacg agttcctgcc ggtgctcgag
     2341 cgcctgcgcc ggtcgcgccg cgagttcgtg ctgtgcggcg actggaacat cgcgcacaag
     2401 gagatcgacc tccggaactg gcgctcgaac cgcaagaact cgggcttcct gcccgaggag
     2461 cgcgagtggc tcaccggcgt cttcgagcgc aagggctggg tcgacgtgtt caggcgcctc
     2521 gatccgcgtc ccgggcagta cacctggtgg tcgacgcgcg gtcgcgcctg ggcgaagaac
     2581 gtcggctggc gcatcgacta ccaggtcgcc accgcgggca tcgcggccaa ggcgacgaag
     2641 gcggcgatct acaaggcgcg gcgcttctcc gaccacgcgc cgctcatcgt cgactacgac
     2701 tggtcgctgg agggtgtcgg cggttgagcc ggcgcgcgcg ccgcgcgcat cgcggcgaac
     2761 gttttcacca ggtcgagcgc gaagcgcccg gtgcgccaga cgctgagcgc gcgcacgacc
     2821 cagcgcaacc cgagacgcgg ccgcgccgcg accagcaccg cggtcgagat cgcgagcagc
     2881 gcgggatgcc ggcgcgccca ctggccccag acgaacgcgc ggtcggcgat cgacaggacg
     2941 ctgcggcagc cggcgagcga ggtcgcgatg gcggcgcgct gctcggccgc gcgcgcgacc
     3001 aggcgctcgc gcttcagcgc gagctcatcg agacgggtca cggggctggg cctgctcgcg
     3061 gtccttgcgc agctccgcga gcgtggccga gaacaggcgc ggacgccgcg ccgcggtgct
     3121 cctcgcggcg agcgcgagcc cggcggcgaa ggcggcgaac acgccggcga gcacgccgat
     3181 cgcgagcagg cggttcgcgt cccagaacag gacgaccagg agcaggacga cgaggttgat
     3241 cgcgagcgcg aggcagaagc cggcgagcgc cgcgaggatc gccatgcgcg cgatccaggc
     3301 gcgctcctcc tcgatgtcgg tggcgaccag ctcgagccgc gtctgcacga cggcgatcag
     3361 cgtcgccgcg agactgcgca gcgagtcgag cacgccgacg gcccggcccg cttcgtcgct
     3421 catcgcgcta cttgcggctg accatcatcc cgagcacgag gccgaccagg gccgcgatgc
     3481 cgaccgactg ccagggatga tcgtggacgt aatcgtcggt ggcgtgcacc gcggcgcgcg
     3541 cctggtccgc cgcctcctcg cccaggagct cgagcttgag cttggccgag tccaggctgt
     3601 cctgcacgcg cgcgcgcgcg gcggcgacct tctcgcccgc ctggttggcg gtggcgcgca
     3661 gcagctcctc ggcgtcgccg atgattctgc gcagatccgt cgccagcttg tcgttggcga
     3721 tgtcgttcgt ggattgcatg agagacctct tgcgggtgag cgatcgaccg gcagaccggc
     3781 cccctctccg tcccggagcc tagccggttt cccatgacgg atcaaccgcg cgtgcggccg
     3841 ggatggttac aatcgcgcat gcccagcgat gcccccccga ccgggaagac ctcgtgggcc
     3901 cgggcgctgc tcaaccggcg catgctgatc tgcgtcttca ccgggttctc ctcgggcctt
     3961 ccgctctacg tcctgatcag cctggtgccg gcgtggctga agaccgaggg gctctcgctc
     4021 gcggtgatcg gcggcttcgc gctcatccag tttccctaca cctggaagtt cctgtgggcg
     4081 ccgctcgccg accgctattc gctcccctgg ctcgggcgcc ggcgcagctg gatgctgctc
     4141 gcgcagctcg gggtgctcgc gtgcatctgg gcgctcggga cgctctcgcc cgcgcgcgac
     4201 ctgcagggcg tgatcgcgat cacggtcgcg gtcgcgctgc tcggcgcgac gctcgacatc
     4261 gcgctcgacg cgtaccggcg cgagctgctg cgcgaggagg agctcggcct cggcaacgcc
     4321 gtgcacgtga acgcgtaccg gatcgcgggc ctcgtgcccg gctcgctcgc gctgatcctc
     4381 gccgatcact acgcctggga tttcgtgttc gcggtcacgg cggcgttcat gcttcccggc
     4441 gtcgtcatgg cgctcgtcgt cgccgagccg gccacgcccg cgggtgcgcc gcggacgctg
     4501 cgcgaggccg tggtcgagcc cttccacgag ttcatgacgc gccacggctg gcgcgaggcc
     4561 ctgttcgtcc tcgccttcgt gttcctgtac aagctcggcg atgcgatgtg cacggcgctc
     4621 gccacgccgt tctatctcga catggggttc tccaagtccg agatcgggct ggtggcgaag
     4681 cacgcgggcc tgtggccgag cgtgatcggc ggcctgctcg gcgggctgtg gatggtgcga
     4741 ctcggcatca accgcgcgct gtggctgttc ggcgtggtgc agctggtgtc gatc
//
LOCUS       DATDMW010000012         2387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1620150, whole genome shotgun sequence.
ACCESSION   DATDMW010000012 DATDMW010000000
VERSION     DATDMW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620150"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VI319_00370"
     CDS             <1..332
                     /locus_tag="VI319_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HEY6862329.1"
                     /translation="SPRAPGSLAAHYAPRTPARLLGSAELRAAVSSNPAAHIAVLAHS
                     MAQPPAFEGVWFDAPAHDAAYAHELYANLRALDALAADEIWIEAPPDGPEWSAVRDRL
                     RRATHRG"
     gene            344..1498
                     /locus_tag="VI319_00375"
     CDS             344..1498
                     /locus_tag="VI319_00375"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY6862330.1"
                     /translation="MAAPHPIVVLAPNSFKGSLSAMDAAAAMAAGIRRVWQHAEVRLR
                     PMADGGDGTLDAVLSIDGERRTARVAGATGEPLDAAYGLIGTDTAIVESAQVVGLTDT
                     RATAQAVERRHTRGLGELVSLLLDAGVREFLIGLGGSSTNDGGAGLLSALGMDLLDAA
                     AKPIDPTPAGLASLARVDARRLDARITQARFTVLSDVDNPLCGERGATAVFGPQKGVR
                     AEAVSEIDSNLARFAKLAEAALGVKAMQRAGAGAAGGLGFALLLLGANIRSGAEVVAD
                     LLGLDAALAGADWLITGEGRSDRQTLLGKAPLVAARRAQAQGVPATLLSGAIERSDLS
                     ELSRVFAGCESIVFGPTTIEACVSEAAALIADRAEQLARLFAAARNALPR"
     gene            complement(1495..>2387)
                     /locus_tag="VI319_00380"
     CDS             complement(1495..>2387)
                     /locus_tag="VI319_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY6862331.1"
                     /translation="DPDVHHAREALAELEMLVVQDIFLTETGYLADVILPASAFPEKT
                     GTFTNTDRAVQLGRQALAPPGEARQDLWLIVEMAKRIGLDWRYSHPREVFEEMRRAMP
                     SIAGITWERLERESAVTYPCEKEGDAGQRVVFTDQFPTATGRGRFVPASIIPAAERPD
                     AQYPFVLITGRQLEHWHTGSMTRRAEVLDAIEPAPVASLHSKDLAALGVAPGGTISVA
                     SRRGRITLSARADDGTPRGAVFIPFCYYEAAANLLTNPVLDPFGKIPEFKYCAVRVSP
                     GGAPAASTQGYSEGVRTAAD"
BASE COUNT          337 a          833 c          853 g          364 t
ORIGIN      
        1 agtcaccgcg cgcgcccggg tccctcgcgg cgcactacgc gccgcgtacg ccggcgcgcc
       61 tgctcggctc cgccgagctt cgcgccgcgg tttcgagcaa tcctgccgcg cacatcgcgg
      121 tgctcgcgca ctcgatggct cagccaccgg cgttcgaggg cgtgtggttc gacgcacccg
      181 cacacgacgc ggcctacgct cacgagctct acgcgaacct gcgcgcgctc gacgcgctcg
      241 cagccgacga gatctggatc gaggcgccgc ccgacggccc ggagtggagc gccgtgcgcg
      301 accggctgcg gcgagcgacg caccgcggct agggtccaga gcgatggccg cgccccatcc
      361 gatcgtcgta ctggccccca attcgtttaa gggctcgctc tcggcgatgg acgcggctgc
      421 ggcgatggcc gcgggaatcc gtcgggtgtg gcagcacgcg gaggtgcggt tgcgtccgat
      481 ggccgatggc ggcgacggca cgctggacgc agttctgagc atcgacggcg agcgccgcac
      541 ggcgcgcgtc gcgggtgcga cgggcgagcc gctcgacgcc gcgtacggct tgatcgggac
      601 cgataccgcg atcgtcgaaa gcgcgcaggt cgtcggactg accgacacgc gagccaccgc
      661 gcaggccgta gaaaggcgcc acacgcgcgg cctgggtgag ctcgtgtcat tgctgctcga
      721 cgcgggcgtg cgcgaatttc tcatcggcct gggcggcagc agcaccaacg acggtggcgc
      781 cgggcttctc tcggcgctcg gcatggattt gctcgatgcc gcggcaaagc caatcgaccc
      841 tacaccggcg ggcctggcat cgctcgccag agtggacgcc cgccggctcg acgcccgtat
      901 cacgcaagcg cgcttcaccg ttctgtccga cgtcgacaat ccgctttgcg gcgagcgcgg
      961 cgcgacggcc gtcttcggcc cgcagaaagg cgtgcgcgcc gaagcggtga gtgaaatcga
     1021 ttctaatctc gctcgcttcg ccaagctcgc cgaagccgca ctcggcgtga aggcgatgca
     1081 gcgagccggc gcaggtgcgg ccggcggtct tggcttcgcg ctacttctgc tcggcgccaa
     1141 catccgctca ggtgccgagg tcgtagccga tctgctcggt ctggacgcgg cgctcgctgg
     1201 cgccgattgg ctcatcaccg gggaaggccg cagcgatcgg cagacgctgc tgggcaaggc
     1261 gccgctggtc gcggcgcgac gggcgcaggc gcagggcgtt ccggcgacgc tgctctcggg
     1321 cgcgatcgag cgcagcgacc tctccgagct ttcgcgcgtt ttcgccggct gcgaatcgat
     1381 cgtcttcggg ccgacgacga tcgaagcctg cgtcagcgag gccgccgcgc tgatcgccga
     1441 tcgcgcggag cagctcgcac ggctcttcgc ggccgcgcgt aatgcgctcc cgcgctagtc
     1501 ggccgccgtc cggacgccct cgctatagcc ctgcgtcgac gcggcaggcg ctccacccgg
     1561 gctcactcgt acagcgcaat acttgaactc cgggatcttg ccgaacggat cgagcaccgg
     1621 gttggtgagc aggttcgccg ccgcctcgta gtagcagaac gggatgaaca cggcgccgcg
     1681 cggcgtgccg tcgtcggcgc gcgcggaaag cgtgatcctg ccccggcgcg aggcgacgct
     1741 gatcgttccg cccggggcga cgcccagcgc ggcgagatcc ttcgaatgca gcgacgcgac
     1801 cggcgcgggc tcgatcgcgt cgaggacttc ggcccggcgc gtcatgctgc ccgtgtgcca
     1861 atgctcgagc tgccgtccgg tgatgagcac gaaggggtat tgcgcatcgg gccgttcggc
     1921 cgcgggaatg atgctcgcgg gaacgaagcg gcccctgccc gtcgcggtcg gaaactgatc
     1981 ggtgaagacc acgcgctgtc cggcgtctcc ttccttctcg cagggatacg tcaccgcgga
     2041 ctcgcgctcc agccgctccc aggtgatgcc ggcgatcgac ggcatggccc ggcgcatctc
     2101 ctcgaagact tcgcgggggt ggctgtagcg ccagtcgaga ccgatgcgct tcgccatctc
     2161 gacgatgagc cacaggtcct ggcgcgcttc tcccgggggc gcgagggcct gccggccgag
     2221 ctgcaccgcg cgatcggtgt tggtgaaggt cccggtcttc tccgggaacg cggaggccgg
     2281 aaggatgacg tccgccagat agcccgtctc ggtgaggaat atgtcctgca cgaccagcat
     2341 ctcgagctcg gcgagggctt cccgcgcgtg atgcacgtcg gggtcgg
//
LOCUS       DATDMW010000013         9055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1744699, whole genome shotgun sequence.
ACCESSION   DATDMW010000013 DATDMW010000000
VERSION     DATDMW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744699"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VI319_00385"
     CDS             <1..526
                     /locus_tag="VI319_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY6862332.1"
                     /translation="IKSRMLLPKPPSAEGDEEDPRAELVRRLLEYERMKSAAQKIDDL
                     PHAGRDFMPVEVWVEQTLAERLPEVHLPDLQDAWRTLLVRAKMTQHHRVTRDELSVRE
                     HMSLILRRLADGTFVEFGALLETRRGLAVLIVNFLAVLELVRESLIEVTQSEPYAPIY
                     VKIAGSAIVHAQSE"
     gene            510..1052
                     /gene="scpB"
                     /locus_tag="VI319_00390"
                     /pseudo
     CDS             510..1052
                     /gene="scpB"
                     /locus_tag="VI319_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            1138..1833
                     /locus_tag="VI319_00395"
                     /pseudo
     CDS             1138..1833
                     /locus_tag="VI319_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            1979..2434
                     /gene="rimP"
                     /locus_tag="VI319_00400"
     CDS             1979..2434
                     /gene="rimP"
                     /locus_tag="VI319_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY6862333.1"
                     /translation="MSALEQLLDRTVTGLGYRLADFDFSNRNRLLRVFIEKTDGTGND
                     AASGVGLDDCEIVTRQLQRVLPVEGIDYDRLEVSSPGLDRRLKSESDFERFAGHRADV
                     RLRVPVNGRRNFVGTLRGVRNGRVELDFDGGHLDFDLADMDRARLVPEL"
     gene            2461..3945
                     /gene="nusA"
                     /locus_tag="VI319_00405"
     CDS             2461..3945
                     /gene="nusA"
                     /locus_tag="VI319_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY6862334.1"
                     /translation="MNRELLLLVDALAREKNVHKETVFGALEAAIAQATKKRFTEDAD
                     VRVAMDRNTGDFESFRRWQVVPDDAPEITPPRQIPLSQAKKEHGDEINVEDFIEEPLE
                     PIDFGRIGAQAAKQVILQKIRDAEREQLLADFLGRGEKLINGAIKRIERGNAIIEVGR
                     VEAVLPRDQMIPKENLRVGDRVRAFVMRIDRAQRGPQLILSRTAPEFIIELFRLEVPE
                     IEEGLLEIKSAARDPGIRAKIAVHSKDARIDPIGTCVGMRGSRVQAVTQELAGERVDI
                     VLWSQDPAQFVIGALAPAEVSSILVDEDKHSMDVVVDEENLAIAIGRSGQNVRLASEL
                     TGWTINLMTEEESQKKNEEETSSVRKLFMERLDVDEEVANILIQEGFSTLEEVAYVPI
                     NEMMEIEGFDEATVNELRSRARDALLVQAIASEEQAENLDPELVNLEGMDPDLAKKLA
                     RGGVKTRDDLGDLAVDDLVEMTAIDTERAKALIMKARAHCFTHE"
     gene            4022..6469
                     /gene="infB"
                     /locus_tag="VI319_00410"
     CDS             4022..6469
                     /gene="infB"
                     /locus_tag="VI319_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY6862335.1"
                     /translation="MLRAAGVHKKVAEDVLSEQDKTKLLDYLRKAHGSSEPKNKITLT
                     RKHSSEIIKRSDASGKARTIQVEVKKKRVFVKRDLSDTGAIPVEAAAPAVDEHEAEIR
                     EQERARQEELAARQAAELAEKQERERRQAETAQKQQAEAKPAAAPGAPAGTTLHKPKV
                     AESGTEKKTAKKKEVKVWKDEGVKRRTIKTRGDVQGASGWHNPKGGGRHRAGGGTVAE
                     QAQQFSAPSEPIVREIHVPETITVGDLAHKMSVKAAEVIKILMKLGSMVTINQVLDQE
                     TAMIVVTEMGHSAKAAKLDDPESLLADESPEHHEAEQMPRAPVVTVMGHVDHGKTSLL
                     DFIRRTRVASGEAGGITQHIGAYHVKTPRGMITFLDTPGHEAFTAMRARGAGVTDIVV
                     LVVAADDGVMPQTVEAIHHAKAAEVPIVVAINKIDKHEANPEKVKQELVAQTVVPEEY
                     GGDSPFVPVSAKTGAGIDALLEQVLLQAEVLELKAPVDAPAKGLVIEARLDKGRGPVA
                     TVLVQSGTLRRGDVVLAGTSFGRVRAMLDENGDAVAEAGPSIPVEIQGLSDVPAAGED
                     VMALADERKAREIALFRQGKFRDVKLAKKQAAKLEDVFDQMKEGGVKVLPLLIKADVQ
                     GSQEALIHALSKLSTEEVKVNVMHSAVGAITESDVNLALASKAVIIGFNTRADAAARK
                     LAEASGVDIRYYNIIYDAVDEVKAALSGMLAPEKKENVLGLVEVRQVFRISKVGTVAG
                     CYVLEGLVRRNGLVRVLRDNVVVYTGELDSLKRFKDDVREVKAGFECGMSVKGYNDIQ
                     VGDQFEVYEVQEVARTL"
     gene            6537..6896
                     /gene="rbfA"
                     /locus_tag="VI319_00415"
     CDS             6537..6896
                     /gene="rbfA"
                     /locus_tag="VI319_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY6862336.1"
                     /translation="MANPQRLRRIADQIQRELSDILRNELKDPRVGMITLTGVEVSPD
                     VAHARVFFTTLGDAAALAATEEGLRRAAGFTRSALGRRLKLHNIPELHFEYDASVERG
                     FRLSKLIDEAVSEKKTG"
     gene            6902..7792
                     /gene="truB"
                     /locus_tag="VI319_00420"
     CDS             6902..7792
                     /gene="truB"
                     /locus_tag="VI319_00420"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY6862337.1"
                     /translation="MPGRDLDGVLLLDKPSGISSNAALQSARRLLGARRAGHTGTLDP
                     MATGLLVLCFGEATKFSAALLDADKAYAARVRLGERTSTGDAEGEVLERREVAVASAG
                     VDTALARFRGAIRQVPPMHSALKHQGRPLYAYARKGQSIERAPREVFVRELVLERLEG
                     RDLDLLVRCSKGTYVRTLAEDLGEALGCGGHLSALRRTAVGPFGIEDALTLDRLATLP
                     EAERARALLPVEVLLRSWPRVLLAKDAETRFRHGQRVAAAAREPRVAVFGADGNFLGI
                     GEIDAEGMLKAKRQMAASAE"
     gene            7868..8137
                     /gene="rpsO"
                     /locus_tag="VI319_00425"
     CDS             7868..8137
                     /gene="rpsO"
                     /locus_tag="VI319_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY6862338.1"
                     /translation="MPVTVAQKAQIVQQHQRATGDTGSPEVQVALLTARINGLTDHFK
                     THVKDHHSRRGLLKLVSQRRKLLDYLKRSDVDRYQKLIEQLGLRK"
     gene            8232..>9055
                     /locus_tag="VI319_00430"
     CDS             8232..>9055
                     /locus_tag="VI319_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY6862339.1"
                     /translation="MTPIKKSFTYGAHQVTLETGEIARQAHGAVLVNMDDTVVLCTVV
                     GAKEARQGQDFFPLTVDYVEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLF
                     PDGFYNEIQVVAQVLSSNNEIDPDIPAMIGVSAALTISGVPFNGPIGGARVGYIDGQY
                     VLCPTNTELKEKSKLNLVVAGTEHAVLMVESEASELPEDVMLGAVVFGHQQMQAAIGA
                     INELADQAAKPAWDWKPAARDEALAERIRSSSEAELRNAFKIRSKRARSEAIDAIWT"
BASE COUNT         1577 a         3054 c         3163 g         1261 t
ORIGIN      
        1 gatcaagtcg cgcatgctgc tgcccaagcc gccgtccgcc gagggcgacg aggaggatcc
       61 ccgcgccgag ctggtgcggc gcctgctcga gtacgagcgc atgaagagcg ccgcgcagaa
      121 gatcgacgac ctgccgcacg cggggcgcga cttcatgccg gtcgaggtct gggtcgagca
      181 gacgctcgcc gagcgcctgc ccgaggtgca cctgcccgac ctgcaggacg cgtggcggac
      241 gctgctcgtg cgcgcgaaga tgacccagca ccatcgcgtg acgcgcgacg agctgtcggt
      301 gcgcgagcac atgagcttga tcctgcgccg gctcgccgac ggcacctttg tcgagttcgg
      361 cgcgctgctc gagacgcgcc gcggcctcgc cgtgctcatc gtcaacttcc tcgcggtgct
      421 cgagctggtg cgcgagagcc tgatcgaggt gacccagtcg gagccctacg cgccgatcta
      481 cgtgaagatt gccgggagcg cgatcgtcca tgctcagtcc gaatgaagta aaagtggtgc
      541 tcgaggcggc gctgctcgcc gcgcgcgagc cgatcgcggt cggcgagctg cgcaagatgt
      601 tcgacgagga ggtcggcgcg gacacggtgc gccggctcct cgacgagctg cgcgagtcct
      661 ggaaggaccg cggtgtcgag ctcgtgaaca ccgcgagcgg ctggcgcttc cagacgcgcg
      721 cctcggtgca gcgctacatc gagcgcatcc accccgacaa gccgccgcgc tactcgcgcg
      781 cggtgctgga gacgctcgcg atcatcgcct accggcagcc ggtgactcgc ggcgacatcg
      841 agaacgtccg cggcgtcacc gtgtcgaccc agatcgtgaa gacgctcgaa gaccgcggct
      901 ggatcgacgt cgtcggccac cgcgaggtcc cgggccgccc ggcgctgtac gccaccacca
      961 agcagttcct cgacgacctc gggctgcgct cgctgcagga gctgccgccg ctcgaggaga
     1021 tcgcgaagac ccttgagctt ccgcaacaag ccgagacgcc cgctccgcaa gccgtcgcgc
     1081 ccggcgagcc cgctccggcc gagcagagcc cggcgtagcc ttccgccgcc ggccccggcg
     1141 gagcggctgc acaaggtgct cgcgcgctcc gggcagggct cgcgccgcga catggaaacg
     1201 ctcatcgcgg cgggccggat caccgtgaac ggccggccgg cgacgctcgg ccagcgtgtc
     1261 gggccggaag accgcgtggc ccgcgacggg cggcccgtgc ggctcgcctt tgcgacgaaa
     1321 acgccgcgcg tgctgctcta ccacaagccg gcgggcgagc tggtcaccga gcgcgacccc
     1381 gagggccgtc ccaccgtgtt cgcgcggctg cctcgcatcc gcggcgggca ttgggtggcg
     1441 atcggccggc tcgatttcaa caccggcggc ctgctgctct tcaccgactc gggcgagctg
     1501 gcgaaccgcc tgatgcatcc ccggcacgag gtcgagcgcg agtacgcggt gcgcatccgc
     1561 ggcgagctct ccgaggctca gctcgagcgt ctcgcgcgcg gcgtccagct cgacgacggt
     1621 ctcgcgcgct tcgacacgat cgagccgcgc ggcgggcagg gctcgaaccg ctggtacgaa
     1681 gtggtgctgc gcgagggccg caaccgcgag gtgcgccgga tgttcgaggc actcggcgtc
     1741 accgtgagcc ggctgatgcg cgtgcgcttc gggccgttcg tgctgccgcc acggctgaaa
     1801 cgtggtcaat ggctcgagct cgacgccgcc gaagcccacg atctgatcga cgcgctcggg
     1861 gagaccgcgg acacgtagcg agggactgtc tcctcgttgt ttcggttgtg ttttcttgct
     1921 atactcgacc ggctataggg atgggctttc gagcccattt tttatttacg tgggtgggat
     1981 gagcgcattg gagcaactat tggatcggac ggtcaccggg ctcggctatc ggctggcgga
     2041 cttcgatttt tccaatagaa accgtctctt gagggtgttc atcgaaaaga cggacggtac
     2101 cggcaacgac gccgcgagcg gcgtgggcct ggacgactgc gagatcgtga cgcggcagct
     2161 gcagcgggtg ctgccggtcg aaggcatcga ctacgaccgg ctcgaagtgt cctcgccggg
     2221 gctcgaccgg cggctgaaga gcgagtccga cttcgagcgt ttcgcggggc accgggccga
     2281 tgtgcggctg cgcgtgccgg tcaacggccg gcgcaacttc gtcggcacgc tgcgcggcgt
     2341 gcgcaacgga cgcgtcgagc tcgacttcga cggcggccac ctcgatttcg acctggccga
     2401 catggaccgc gcccgcctgg tgcccgagct gtgagcgccg ctgtgagagt ggagggacaa
     2461 atgaaccgag agctgctgct gctggtcgac gcgctggcgc gcgagaagaa cgtccacaag
     2521 gagacggtct tcggcgcgct cgaggccgcg atcgcgcagg cgaccaagaa gcgcttcacc
     2581 gaggacgccg acgtgcgcgt cgcgatggac cgcaacaccg gcgacttcga gtcgttccgg
     2641 cgctggcagg tggtgcccga tgacgccccc gagatcacgc cgccgcgaca gatccctctg
     2701 tcgcaggcga agaaggagca cggcgacgag atcaacgtcg aggacttcat cgaggagccg
     2761 ctcgagccga tcgacttcgg ccgcatcggc gcgcaggccg cgaagcaggt gatcctgcag
     2821 aaaatccgcg acgccgagcg cgagcagctg ctcgcggact tcctcggccg cggcgagaag
     2881 ctgatcaacg gcgcgatcaa gcgcatcgag cggggcaacg ccatcatcga ggtggggcgc
     2941 gtggaggcgg tgctgccgcg cgaccagatg atcccgaagg agaacctgcg cgtgggcgac
     3001 cgcgtgcgcg ccttcgtgat gcggatcgac cgcgcgcagc gcggcccgca gctgatcctg
     3061 tcgcgcaccg cgcccgagtt catcatcgag ctgttccgcc tcgaggtgcc cgagatcgaa
     3121 gaggggctgc tcgagatcaa gtcggccgcg cgcgaccccg gcatccgcgc caagatcgcc
     3181 gtgcactcga aggacgcgcg catcgatccg atcggcacct gcgtcggcat gcgcggctcg
     3241 cgcgtgcagg cggtgacgca ggagcttgcg ggcgagcgcg tcgacatcgt gctctggtcg
     3301 caggatccgg cgcagttcgt gatcggcgcg ctcgcgccgg ccgaagtgtc gtcgatcctg
     3361 gtcgacgagg acaagcacag catggacgtg gtggtcgacg aggagaacct cgcgatcgcg
     3421 atcggccgca gtggccagaa cgtgcgcctc gcctccgagc tgaccggctg gaccatcaac
     3481 ctcatgaccg aggaagagtc gcagaagaag aacgaggagg agacctcctc ggtgcgcaag
     3541 ctcttcatgg aacggctcga cgtcgacgag gaggtggcga acattttgat ccaggaaggc
     3601 ttctcgaccc tcgaggaagt cgcttacgtc ccgatcaacg agatgatgga gatcgagggc
     3661 ttcgacgagg ccacggtcaa cgagctgcgc tcgcgcgcgc gcgacgcgct gctggtgcag
     3721 gcgatcgcga gcgaagagca ggccgagaac ctcgaccccg agctcgtcaa cctcgagggc
     3781 atggacccgg acctcgcgaa gaagctcgcc cgcggcggcg tgaagacgcg cgacgacctg
     3841 ggcgatctcg cggtggacga cctcgtggag atgaccgcga tcgatacgga gcgggccaag
     3901 gccttgatca tgaaggcccg cgcacactgt tttacgcatg agtagccaac aacccatttc
     3961 ccatacgagc gtttcgcaat tcgcagtcga gcttaagttc ccgccgtctc atctgctcta
     4021 gctgttgcgc gcagcgggcg tgcacaagaa ggtcgccgag gacgtgctct cggagcagga
     4081 caagaccaag ctcctcgact acctgcgcaa ggcccacggc tcctccgagc cgaagaacaa
     4141 gatcacgctc acgcgcaagc actcctccga gatcatcaag cgctcggacg cgagcggcaa
     4201 ggcgcgcacc atccaggtcg aggtcaagaa gaagcgcgtg ttcgtgaagc gcgatctttc
     4261 cgatacggga gcgatcccgg tcgaggcggc cgcgcccgcg gtcgacgagc acgaagccga
     4321 gatccgggag caggagcgcg cgcgccagga agagctcgcc gcgcgccagg ccgccgagct
     4381 cgccgagaag caggagcgcg agcgccgtca ggccgaaacg gcgcagaagc agcaggcgga
     4441 ggcgaagccg gccgcagccc cgggggcacc cgcgggcacg acgctgcaca agccgaaggt
     4501 cgccgagagc gggacggaga agaagaccgc caagaaaaaa gaggtcaagg tctggaagga
     4561 cgagggcgtc aagcgccgca cgatcaagac gcggggcgac gttcagggcg cctccggctg
     4621 gcacaacccc aagggcgggg ggcgccatcg cgcgggcggc ggcacggtcg ccgagcaggc
     4681 gcagcagttc tccgcgccgt ccgagcccat cgtgcgcgag atccacgtgc ccgagacgat
     4741 caccgtgggc gacctcgcgc acaagatgtc cgtgaaggcc gccgaggtca tcaagatcct
     4801 gatgaagctc ggcagcatgg tcacgatcaa ccaggtgctc gaccaggaga cggcgatgat
     4861 cgtcgtcacc gagatggggc acagcgccaa ggctgcgaag ctcgacgatc ccgagtcgct
     4921 gctcgccgac gagtcgccgg agcatcacga ggccgagcag atgccgcgcg cgcccgtggt
     4981 cactgtcatg ggccacgtcg accacggcaa gacctcgctc ctcgacttca tccgccgcac
     5041 gcgcgtcgcc tcgggcgagg ccggcgggat cacgcagcac atcggcgcct atcacgtgaa
     5101 gacgccgcgc ggcatgatca ccttcctcga cacgcccggc cacgaggcgt tcaccgccat
     5161 gcgcgcccgg ggcgcaggcg tgaccgacat cgtcgtgctc gtcgtggcgg ccgacgacgg
     5221 cgtgatgccg cagacggtcg aggcgatcca ccacgccaag gcggcggagg tgccgatcgt
     5281 ggtcgcgatc aacaagatcg acaagcacga ggcgaacccc gagaaggtga agcaggaact
     5341 ggtcgcccag acggtcgtgc ccgaagagta cggcggcgac tcgccgttcg tgccggtgtc
     5401 cgcgaaaacc ggcgccggca tcgacgccct gctcgagcag gtgctgctgc aagccgaggt
     5461 gctcgagctg aaggcgccgg tcgacgcgcc ggcgaagggc ctggtgatcg aggcgcgact
     5521 ggacaaaggt cgcggcccgg tcgcgaccgt gctggtccag tcgggcacgc tgcgccgcgg
     5581 cgacgtcgtg ctcgccggga cctcgttcgg ccgcgtgcgc gcgatgctcg acgagaacgg
     5641 cgacgccgtg gccgaggccg gcccgtcgat cccggtcgag atccagggcc tgtccgacgt
     5701 gcccgcggcg ggcgaggacg tcatggcgct cgccgatgag cgcaaggcgc gcgagatcgc
     5761 gctcttccgc cagggcaagt tccgcgacgt caagctcgcg aagaagcagg cggcgaagct
     5821 cgaggacgtg ttcgaccaga tgaaggaggg cggcgtgaag gtgctgccgc tcctgatcaa
     5881 ggccgacgtg cagggctcgc aggaggcgct gatccacgcg ctgtccaagc tctccaccga
     5941 ggaggtgaag gtcaacgtga tgcacagcgc ggtcggcgcc atcaccgagt ccgacgtcaa
     6001 cctcgcgctc gcgtcgaaag ccgtgatcat cggcttcaac acgcgcgcgg acgcggccgc
     6061 ccgcaagctc gccgaggcct cgggcgtcga catccgctac tacaacatca tctatgacgc
     6121 ggtcgacgag gtgaaggcgg cgctgtccgg catgctcgcc cccgagaaga aggagaacgt
     6181 gctggggctg gtcgaggtgc gccaggtgtt ccgcatctcg aaggtcggca cggtcgcggg
     6241 ctgctacgtg ctcgagggcc tcgtgcgccg caacggcctg gtgcgcgtgc tgcgcgacaa
     6301 cgtcgtcgtc tacaccggcg agctcgactc gctcaagcgc ttcaaggacg acgtacgcga
     6361 ggtgaaggcc ggcttcgagt gcggcatgtc ggtgaagggc tacaacgaca tccaggtcgg
     6421 ggaccagttc gaggtctacg aggtgcaaga ggtggcgcga acgctgtagc gttcgcgcgg
     6481 tacctcttgc atcctgaggc gcagcgcgcc gaaggatctg cttttccggt cgccccatgg
     6541 ccaaccccca gcgtctccgc cgcatcgccg accagatcca gcgcgagctg tccgacatcc
     6601 tgcgcaacga gctgaaggac ccgcgcgtcg gcatgatcac gctgactggc gtcgaggttt
     6661 ctcccgacgt ggcccacgcc cgggtgttct tcaccacgct gggcgacgcc gccgcgctcg
     6721 ccgcgaccga ggaagggctg cggcgcgccg ccgggttcac gcgcagcgcg ctcgggcggc
     6781 gcctgaagct gcacaacatc cccgagctgc atttcgagta cgacgcctcg gtcgagcgcg
     6841 gctttcgcct gtcgaagctg atcgacgaag cggtgtcgga aaagaagacc ggctgaaccg
     6901 gatgcccggg cgcgacctcg acggggtgct gctcctcgac aagccttccg ggatcagctc
     6961 gaacgccgcc ctgcagagcg cccggcggct gctcggggcg cggcgggcgg ggcataccgg
     7021 cacgctcgac ccgatggcga cagggctgct cgtcctgtgc ttcggcgaag cgaccaagtt
     7081 ctccgccgcg ctgctcgacg ccgacaaggc ctatgcggcg cgggtccggc tcggtgagcg
     7141 cacgtcgacg ggagacgcgg aaggcgaggt gctcgagcgg cgggaggtcg ccgtcgcctc
     7201 ggccggcgtc gacaccgcgc tggcgcgctt ccgcggagcc atccggcagg tcccgcccat
     7261 gcattcggcc ctcaagcatc aggggcgccc gctgtacgca tacgcgcgca aaggccagtc
     7321 gatcgagcgc gcgccgcgcg aggtgttcgt gcgcgagctc gtgctcgagc ggctcgaagg
     7381 ccgcgatctc gacctgctcg tccgctgcag caagggcacc tacgtccgca cgctcgccga
     7441 ggacctcggg gaggccctgg gctgcggcgg gcacctgagc gcgctgcgcc ggacggccgt
     7501 cggtcctttc gggatcgagg acgccttgac cctggaccgc ctcgccacgc tgcccgaggc
     7561 cgagcgagcc cgggcgctgc tgccggtgga ggtcctcctg cggtcgtggc cgcgggtgct
     7621 cctggcgaag gacgccgaga cccggttccg gcacggccag cgcgtggcgg cggccgcccg
     7681 cgagccgcgg gtcgccgtgt tcggggccga cggaaatttc ctcggcatcg gggaaatcga
     7741 cgccgaaggc atgctcaagg cgaagcgcca gatggcggct tccgccgaat gaccggccgc
     7801 ttgaggaagg cgctgatttc aggctaaaat gtgcggcttc cgagaagaga cgagaaagta
     7861 gagaagcatg cccgtgaccg tcgcgcaaaa agcgcagatc gtgcagcagc accaacgcgc
     7921 cacaggcgac accggctcgc ccgaagtgca ggtcgccctg ctcaccgccc gcatcaacgg
     7981 attgaccgac cacttcaaga cgcacgtgaa ggatcatcat tcccgacgcg gactgctgaa
     8041 gctcgtgagc cagcgccgca agctcctgga ttacctgaag cgcagcgacg tcgaccggta
     8101 ccagaagctg atcgagcagc ttggcctccg caagtaactc cgcgacgccc gcccgtatcc
     8161 cgcgcgggcg tttttgattc agaggttcga gcgacgtccc caagacgcgc ccggcattta
     8221 tcgagactgc tatgactccc atcaagaaaa gcttcaccta cggcgcccac caagtgacgc
     8281 tcgagaccgg cgagatcgcg cgccaggccc acggcgccgt gctcgtcaac atggacgaca
     8341 ccgtcgtcct gtgcacggtc gtcggcgcca aggaggcccg ccaaggtcag gacttcttcc
     8401 cgctcacggt cgactacgtc gagaagacct atgccgcggg caagatcccc ggcggcttct
     8461 tcaagcgcga gggccgtccc tcggagaagg agacgctgac ctcgcggctg atcgaccggc
     8521 ccatccggcc gctcttcccc gacggcttct acaacgagat ccaggtcgtc gcccaggtgc
     8581 tctcgagcaa caacgagatc gaccccgaca tcccggcgat gatcggcgtg tcggcggcgc
     8641 tgaccatctc cggcgtcccg ttcaacggcc cgatcggcgg cgcgcgcgtc ggctacatcg
     8701 acggccagta cgtcctgtgc ccgaccaaca cggagctgaa ggagaagagc aagctcaatc
     8761 tcgtcgtcgc cggaaccgag catgccgtgc tgatggtcga gtccgaggcg agcgagctgc
     8821 ccgaggacgt gatgctcggg gcggtggtct tcgggcacca gcagatgcag gcggcgatcg
     8881 gcgcgatcaa cgagctcgcc gaccaggccg ccaagcccgc ctgggactgg aagccggcgg
     8941 cgcgcgacga ggcgctcgcc gagcgcatcc gctccagctc cgaagccgag ctgcgcaatg
     9001 ccttcaagat ccgctccaag cgcgcccgct ccgaggcgat cgacgccatc tggac
//
LOCUS       DATDMW010000014         3512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_126562, whole genome shotgun sequence.
ACCESSION   DATDMW010000014 DATDMW010000000
VERSION     DATDMW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126562"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VI319_00435"
     CDS             <1..351
                     /locus_tag="VI319_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862340.1"
                     /translation="QFKGLDPSGSANHAYYIWVDRYNTLGLGANVPVASANGGESLLA
                     VVDGKLVDIRIPYPLGFFTKLVDGRIDDPSAGWKGRGMWTMSGTRTVFHNEGGTENRP
                     KVYKIQVRPSPLAR"
     gene            complement(361..1452)
                     /locus_tag="VI319_00440"
     CDS             complement(361..1452)
                     /locus_tag="VI319_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6862341.1"
                     /translation="MAIAAKAAEEAYDEGLRRFDAGDLDAAARAFERAVALDPGFERA
                     HYQLGNVRQDQERWTDAERCFRGALALDPGHAEAHNNLGVVLQVLGRSRDAEASYARA
                     AELKPALVHPWLNLGRMLDGMGRRAEAIAWLEKGSALSAEPEAFRHVLAAMRGDAATG
                     RAPDSYVRSTYDGFAAQFDHRLVGELGYRVPEAISAVLADLGRPAPAAADILDLGCGT
                     GLSGWALRGLARSLTGVDLSPKMLEQARARACYDVLAEDEVVAWMRASPASRFDLVVA
                     ADAFIYFGALDAVFAEVARVSRPEALFTFSVESCEGAEWRLQDSGRYAQSDDYIRRLA
                     ADHGFAVAVNREQPIRKPVIGRLYVLERR"
     gene            complement(1511..1843)
                     /locus_tag="VI319_00445"
     CDS             complement(1511..1843)
                     /locus_tag="VI319_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862342.1"
                     /translation="MKALLALSLFTIALASLLLLTRAGDNVRWETLRVYPLSAAADAR
                     SVAVAVPSSWREAGQTSFLGSTLRFVDESGREIAITRADLLQAAAENRVYRPARAEEL
                     MLAQRPAR"
     gene            1985..2944
                     /locus_tag="VI319_00450"
     CDS             1985..2944
                     /locus_tag="VI319_00450"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6862343.1"
                     /translation="MRSLPIALALALAFASISSYAQSPARPVRVLVPFAAGGASDTYS
                     RLAAIKINEQTGKTFVVENRTGAGGRICWEAAAKSAADGTTIALIDATYPMLPGLFAK
                     LPWDAANDLVPAAMIAQTPFVITVSTESKLKSLRELVAEAKARPGKLNYGSSGVGGVN
                     HVVTALFLHDAGINLTHVPYKGMSDASVALQGGQVDLIIAASPTALGSIKGNKARALA
                     VSTAKRSPALPGVPTATEAGVPYVVTNWFGFAVPKGTPAAFIKTLRDDVVRATSAPDV
                     REKLAAQGAEPSAFTPEEMQQFLKDETRRWGEVIREAGIRVEQ"
     gene            complement(2951..>3512)
                     /locus_tag="VI319_00455"
     CDS             complement(2951..>3512)
                     /locus_tag="VI319_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEY6862344.1"
                     /translation="TEAPNHPQKPEWGKRKLPFSRDLWIEREDFSENPPKGYFRLFPG
                     NKVRLRYGFVVECIGYDKATDTVRCTYDPDTKSGTPGSEKVKVKGNIHWVSAKHAHAA
                     EVRLYDRLFKAPHPGAGDRDFLQDLNPDSKKVVTAQLEPALRDAKPEERFQFERHGYF
                     VADRSDSTAGAPKFNRAVTLRDSWQK"
BASE COUNT          521 a         1233 c         1256 g          502 t
ORIGIN      
        1 cagttcaagg gcctcgaccc gtccggcagc gcgaaccacg cgtactacat ctgggtcgac
       61 cgctacaaca cgctgggcct cggggcgaac gtgccggtcg cctcggcgaa cggcggcgag
      121 tcgctgctcg cggtggtgga cggcaagctc gtcgacatcc gcatcccgta cccgctcggg
      181 ttcttcacga agctggtcga cggccgcatc gacgatccgt cggcgggctg gaagggcagg
      241 ggcatgtgga cgatgtcggg gacgcgcacc gtgttccaca acgagggcgg caccgagaac
      301 cggccgaagg tctacaagat ccaggtgcgt ccgagcccgc tcgcgcgctg aggcgcgggg
      361 tcagcggcgc tcgaggacgt acagccgccc gatcacgggc ttgcggatcg gctgctcgcg
      421 gttcaccgcg acggcgaagc cgtggtcggc cgcgagccgg cggatgtagt cgtccgactg
      481 cgcgtagcgg ccgctgtcct gcagccgcca ttccgcgcct tcgcagcttt cgacggagaa
      541 cgtgaacagc gcctcggggc gcgacacgcg cgcgacctct gcgaacacgg cgtcgagcgc
      601 gccgaagtag atgaaggcgt cggcggcgac gacgagatcg aagcgcgacg ccgggctcgc
      661 gcgcatccag gcgaccacct cgtcctccgc gagcacatcg tagcaggcgc gcgcgcgcgc
      721 ctgctcgagc atcttcggcg agagatcgac gcccgtgagg ctgcgtgcca ggccgcgcag
      781 cgcccagccc gagagcccgg tgccgcagcc gagatcgagg atatcggccg ccgccggcgc
      841 ggggcgtccg aggtccgcga gcaccgccga gatcgcctcg ggcacgcggt agccgagctc
      901 gccgacgagg cggtggtcga actgcgcggc gaagccgtca taggtgctgc gtacgtacga
      961 gtcgggcgcg cgccccgtcg ccgcgtcgcc gcgcatggcg gcgagcacgt ggcggaatgc
     1021 ctcgggctcg gcgctcagcg ccgagccctt ctccagccac gcgatcgcct cggcgcgccg
     1081 gcccattccg tcgagcatgc gtccgaggtt gagccagggg tggacgagcg cgggcttgag
     1141 ctcggcggcg cgcgcgtagc tcgcctcggc gtcgcggctc cgtcccagca cctgcagcac
     1201 gacgccgagg ttgttgtgcg cctcggcgtg gcccgggtcg agcgcgagcg cgccgcggaa
     1261 gcagcgctcc gcatcggtcc agcgctcctg gtcctggcgc acgttgccga gctggtaatg
     1321 cgcgcgctcg aagccggggt cgagcgcgac cgcgcgctcg aaggcgcgcg cggcggcgtc
     1381 gaggtcgccc gcgtcgaacc ggcgcagtcc ctcgtcgtag gcctcttcgg ccgcctttgc
     1441 tgcgatcgcc acgtaccatg ctccccgcgg accggggagt atcgcatacc ggacgcagcg
     1501 cccgggccgc ctagcgggcg gggcgctgcg ccagcatcag ctcctcggcg cgcgccgggc
     1561 gatagacgcg gttctcggcc gcggcctgca gcaggtcggc gcgcgtgatc gcgatctccc
     1621 tgcccgactc gtcgacgaag cgcagcgtcg agccgaggaa ggaggtctgg cccgcctcgc
     1681 gccagctcga cggcacggcg acggccacgg atcgggcgtc ggcggcggcg gaaagcgggt
     1741 agacgcgcag cgtttcccaa cggacgttgt cgccggcgcg ggtgagcagc agcagcgacg
     1801 cgagcgcgat cgtgaacaga gacaaggcga gcaaggcttt catcttcggt ctcccggatc
     1861 tggaccgcgc caatatagga gcggcccgga cgccgggatt gacgccggtc aaggttcggg
     1921 cacccgcgcg cggcgggagg atggggtagc atcacgcgat cccccgtcgt gaaggacctt
     1981 gccgatgcgc agcctgccga tcgcccttgc ccttgcgctc gctttcgcga gcatcagctc
     2041 atacgcgcag agcccggcgc gcccggtccg cgtgctcgtg cctttcgccg ccggcggcgc
     2101 gagcgacacc tacagccggc tcgcggcgat caagatcaac gagcagaccg gcaagacctt
     2161 cgtggtcgag aaccgcaccg gcgccggcgg ccgcatctgc tgggaggccg ccgcgaaatc
     2221 cgcggccgac ggcaccacca tcgcgctgat cgacgcgacc tacccgatgc tgcccgggct
     2281 gttcgcgaag ctgccgtggg acgccgcgaa cgacctcgtg cccgccgcga tgatcgcgca
     2341 gacgccgttc gtgatcaccg tgagcaccga atcgaagctg aagtcgctgc gcgagctcgt
     2401 cgccgaggcc aaggcgcggc cggggaagct gaactacggc tcttcgggcg tcggcggcgt
     2461 caaccacgtc gtcaccgcgc tcttcctcca cgacgccggc atcaacctca cgcacgtccc
     2521 gtacaagggc atgagcgacg cgagcgtcgc gctgcagggc gggcaggtcg acctcatcat
     2581 cgccgcgtcg cccaccgcgc tcggctcgat caagggcaac aaggcaagag cgctggcggt
     2641 ctcgaccgcg aagcgctcgc ccgcgctgcc cggcgtgccg accgcgaccg aggcgggcgt
     2701 gccttacgtc gtcaccaact ggttcggctt cgccgtgccg aaggggactc ccgcggcgtt
     2761 catcaagacc ttgcgggacg acgtcgtgcg cgcaacctcg gcccccgacg tgcgcgagaa
     2821 gctcgccgcg cagggcgcgg agccctcggc gttcacgccg gaggagatgc agcagttcct
     2881 gaaggacgag acgcggcgct ggggcgaggt gattcgcgag gcggggatcc gggtcgagca
     2941 gtgagcctgc ctatttctgc caggaatccc ggagcgtcac cgcgcggttg aacttcggcg
     3001 cgccggccgt cgagtcggag cgatcggcaa cgaagtagcc gtgccgctcg aactggaagc
     3061 gctcctcggg cttcgcgtcc ctcagcgccg gctcgagctg cgccgtgacg actttcttcg
     3121 aatcggggtt gaggtcctgg aggaagtcgc gatcgcccgc gccgggatgc ggcgccttga
     3181 acaggcggtc gtacagccgc acctcggccg cgtgcgcatg cttcgcgctc acccagtgga
     3241 tgttgccctt caccttcacc ttctccgagc ccggcgtgcc tgacttggta tccgggtcgt
     3301 aggtgcagcg gacggtatcc gtcgccttgt cgtagccgat gcattcgacg acgaagccgt
     3361 agcgcaggcg caccttgttg ccggggaaca aacggaagta gcccttcggc gggttctcgc
     3421 tgaaatcctc gcgctcgatc cacaggtcgc gcgagaaggg cagtttcctc ttcccccact
     3481 cgggcttctg cgggtggttc ggcgcttcgg tc
//
LOCUS       DATDMW010000015         2675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2367396, whole genome shotgun sequence.
ACCESSION   DATDMW010000015 DATDMW010000000
VERSION     DATDMW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367396"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VI319_00460"
     CDS             <1..769
                     /locus_tag="VI319_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY6862345.1"
                     /translation="AAARAIEDDVGKAVAADSPEQADAREKVEPVTQHKLPYIVVVID
                     EFADLMMCAPKEVETSVARIAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIAFQ
                     VSSRVDSRTILDQQGAESLIGQGDMLYLPPGTGMPTRVHGAYVADHEVHKVVEHLKSL
                     GAPDYIGGVLEGGEAAANGELAPGEHPGGEADPLYDQAVDIVLRTRRASISLVQRHLR
                     IGYNRAARLIEQMEVAGLVSAMQSNGNRDVLVPAKAE"
     gene            830..1459
                     /gene="lolA"
                     /locus_tag="VI319_00465"
     CDS             830..1459
                     /gene="lolA"
                     /locus_tag="VI319_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEY6862346.1"
                     /translation="MTILLRNAFAALVALALAPVACASSLDRFSDFLKGTQSARGGFT
                     QQIRDRSGRVVQESRGTLAFQRPGKFRWVYEKPYPQVIVGDGRKVWIYDEDLNQVTVK
                     RLDKALGSTPAALLAGSNDVAKAFDLSDAGRKDGLEWVAARPRDKEASFERIRIGFGA
                     SGLAAMELSDSFGQTTVLRFHSLQRNPKLDASQFRFSPPKGADVIGDTK"
     gene            1467..>2675
                     /locus_tag="VI319_00470"
     CDS             1467..>2675
                     /locus_tag="VI319_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY6862347.1"
                     /translation="MSGRGSDLFPGPAPASPLADALRPKTLDEVVGQQHLIGPGKPLR
                     LAFESGKPHSMILWGPPGTGKTTIARLMADAFDAEFIALSAVLSGVKDIKEAVQRAQE
                     ALAASGRRTILFVDEVHRFNKAQQDAFLPFVEQGLFTFVGATTENPSFEVNGALLSRA
                     TVYVLKSLTEAELGQLFDRAVRRSADGPRYDASARERVVGWADGDARRLLNLVEILDT
                     AAKSAGVAEVDGAFVENAVARNLRRFDKGGDAFYDQISALHKSVRGSSPDAALYWLVR
                     MLDGGADPLYLGRRIVRMAIEDIGLADPRALRIALDACETYERLGSPEGELALAEAVL
                     YLACAAKSNAVYEAYNSARAFVADDQSRPVPVHLRNAPTKLMKELDYGKGYRYAHDEP
                     GAFAKGERYLP"
BASE COUNT          411 a          962 c          934 g          368 t
ORIGIN      
        1 cgccgccgcg agggcgatcg aggacgacgt cggcaaggcg gtcgccgccg acagccccga
       61 gcaggccgac gcgcgcgaga aggtcgagcc ggtgacgcag cacaagctgc cgtacatcgt
      121 cgtcgtgatc gacgagttcg cggacctgat gatgtgcgcg ccgaaggaag tggagacgtc
      181 ggtcgcgcgc atcgcgcaga aggcgcgcgc cgccggcatc catctcatcc tcgccacgca
      241 gcgcccgtcg gtggacgtga tcaccgggct gatcaaagcc aacatcccga cccgcatcgc
      301 gttccaggtc tcgagccgcg tcgactcgcg caccatcctc gatcagcagg gcgccgaatc
      361 gctgatcggg cagggcgaca tgctgtacct gccgcccggc acgggcatgc cgacgcgcgt
      421 ccacggcgcg tacgtcgccg atcacgaggt gcacaaggtc gtcgagcatc tgaagtcgct
      481 gggcgcgccc gactacatcg gcggcgtgct cgagggcggg gaggcggcgg cgaacggcga
      541 gctcgccccg ggcgagcacc ccggtggcga ggccgacccc ctctacgacc aggcggtcga
      601 catcgtcctg cgcacccggc gcgcgtccat ctcgctggtg cagcggcatc tgcgcatcgg
      661 ctacaaccgc gcggcgcgcc tgatcgagca gatggaggtc gcgggtctcg tgtccgcgat
      721 gcagtcgaac ggcaaccgcg acgtcctggt gcccgccaag gccgagtagg gcgcgcgccc
      781 ggcgcgcctc ggccgggcac gtataatcgg gcccgctgtc ccaccctcca tgacgatcct
      841 gctccgcaac gccttcgccg cgctcgtcgc cctcgcgctc gcccccgtcg cgtgcgcctc
      901 gagcctcgat cgattctccg atttcctcaa gggcacgcag tcggcgcgcg gcggcttcac
      961 gcagcagatc cgcgaccgca gcgggcgcgt ggtccaggag tcgcgcggga cgctcgcgtt
     1021 ccagcggccg gggaagttcc gctgggtcta cgaaaaaccc tatccgcagg tcatcgtcgg
     1081 cgacggccgc aaggtctgga tctacgacga ggacctgaac caggtcaccg tgaagcgcct
     1141 cgacaaggcg ctcggatcga cgccggccgc gctcctcgcc ggcagcaacg acgtggcgaa
     1201 ggcgttcgac ttgtcggacg cggggcgcaa ggacggcctc gagtgggtgg cggcgcgccc
     1261 cagggacaag gaggcgagct tcgagcgcat ccggatcggc ttcggggcct cggggctcgc
     1321 ggcgatggag ctctccgaca gcttcggaca gacgacggtg ctgcggttcc attcgctcca
     1381 gcgcaacccg aagctcgacg cgtcgcagtt caggttctcg cccccgaaag gcgcggatgt
     1441 catcggcgac acgaagtgac cgcgccgtga gcggccgcgg gagcgacctt ttccccgggc
     1501 cggcgcccgc atcgccgctc gccgacgcgc tgcggccgaa gacgctcgac gaggtggtcg
     1561 ggcagcagca cctgatcggg ccggggaagc cgctgcgcct cgcgttcgag tcgggcaagc
     1621 cgcattcgat gatcctttgg gggccgcccg gcaccggcaa gacgacgatc gcgcgcctga
     1681 tggccgacgc cttcgacgcc gagttcatcg cgctctcggc cgtgctgtcg ggcgtcaagg
     1741 acatcaagga ggccgtgcag cgcgcgcagg aggcgctcgc ggcctcgggc cggcgcacca
     1801 tcctgttcgt cgacgaggtg caccgcttca acaaggcgca gcaggacgcg ttccttccgt
     1861 tcgtcgagca gggcctcttc accttcgtcg gcgcgacgac cgagaacccg tcgttcgagg
     1921 tgaacggcgc gctgctctcg cgcgcgacgg tgtacgtgct gaagagcctc acggaggccg
     1981 agctcggtca gctcttcgac cgcgcggtgc ggcgctcggc cgacggcccg cgctacgacg
     2041 cgagcgcgcg cgagcgcgtc gtcggctggg cggacggcga cgcgcggcgc ctgctcaacc
     2101 tggtcgagat cctcgacacg gccgcgaaga gtgccggcgt ggcggaggtc gacggcgcgt
     2161 tcgtcgagaa cgcggtcgcg cgcaacctgc gccgcttcga caagggcggc gacgccttct
     2221 acgaccagat ctcggcgctg cacaagtcgg tgcgcggctc gagccccgac gccgcgctgt
     2281 actggctcgt gcgcatgctc gacggcggcg ccgatccgct ctacctcgga cggcgcatcg
     2341 tgcgcatggc gatcgaggac atcgggctcg cggatccgcg cgcgctgcgc atcgcgctcg
     2401 acgcctgcga aacctacgag cggctcggca gcccggaagg cgagctcgcg ctcgccgagg
     2461 cggtcctcta cctcgcctgc gccgcgaaat ccaacgccgt ctacgaggcc tataattcgg
     2521 cgcgcgcctt cgtcgccgac gatcaatccc gccccgtgcc cgtgcacctc cgcaacgccc
     2581 cgacgaagct catgaaggag ctcgactacg gcaaggggta ccgctacgcg cacgacgaac
     2641 cgggcgcgtt cgcgaaaggc gagcgctatc taccc
//
LOCUS       DATDMW010000016         5990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_126864, whole genome shotgun sequence.
ACCESSION   DATDMW010000016 DATDMW010000000
VERSION     DATDMW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126864"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1014)
                     /locus_tag="VI319_00475"
     CDS             complement(49..1014)
                     /locus_tag="VI319_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6862348.1"
                     /translation="MGNAVPPGDRAGLRMKLRDLHVLMTVAQARSMSKAAALLHTTQP
                     AVSRSIAELEGALGVRLLERSVRGVEPTAYGRAVLEGGVAVFDELRRAVRNVEFLADP
                     AAGEVRIGCTPILAASFVSAVVDRLSRRYPRMVFHLVTAYAEPLRRALLAREVDFLVV
                     RRFAPAAEEQLEFEPLFDDSYVVAAGAKSRWVRRRRIELAELAGEAWTLPPPGSAIAS
                     IAMEAFRAAGLDYPRTTVVCDSPETRISLLGTGRFVTIFPASISRFPTLRPEIRILPV
                     ALPRARVQNVIVTLKGRALSPVAKLFIERAREAARADRAGRRRGG"
     gene            1104..2081
                     /locus_tag="VI319_00480"
     CDS             1104..2081
                     /locus_tag="VI319_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862349.1"
                     /translation="MTFSRRRFLNLAAGAAALAAVPRLARAQAYPSRPVRIVVGFSAG
                     GPNDVLARLIGERLSQRLGQPFVVENRPGAGGNIATEAVVRAPADGYTLLLVGSPNAI
                     NATLYDKLGFDFIRDIAPVAGLMRGDLVMVVHPSVPAATLPELIAYAKAHPGKLSYGS
                     AGVGGITHITAELFKMMAGVDIVHVPYRGVAPALTDLLAGEVQVVFTNPGQTVQYTRA
                     GRLRALAITTAKRSSAFPDIPTVGESVPGYEAGSLFGIGAPKNTPAEIVEKLNGEIDA
                     ALADPGFRTRLAKLDGTVLGGSPADFGRLVAAETEKWGKVIKFAKIRPD"
     gene            2059..2799
                     /locus_tag="VI319_00485"
     CDS             2059..2799
                     /locus_tag="VI319_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6862350.1"
                     /translation="MRKSGPTDLEAATGFLNIRSRHGGEFACYLAAPAIGGPVPAVVL
                     ASAVHGVDADLRAIADDFAAHGFIAAAPDLFWRSIPGPLSRDDERTAQRSRPRLEKIR
                     IGESDMADTLAYLGTLPNFNGRAAAMGFCYGGPYAILGPKRLGYAAGISCHGSQMLDY
                     IAELDGVGAPVCILWGDRDHQAPAPVLEAYRALASRRKNVEVHVFPGVLHGYMMPGAQ
                     AFHPATRELSMQRARAILDGLKGAPPRG"
     gene            2821..>5990
                     /gene="uvrA"
                     /locus_tag="VI319_00490"
     CDS             2821..>5990
                     /gene="uvrA"
                     /locus_tag="VI319_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY6862351.1"
                     /translation="MQHRKSGAAITIRGARQNNLKNLTLEIPTNELVVVTGVSGSGKS
                     SLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVDAIEGIPPAIAIDQTNPVRTSRS
                     TVGTMTELNDHLKLLYARAAGLYCRGCGRAVRRDTPESIYAEIAARAAKEGDPRLIVA
                     FPVPIPKNFKADEIEAALKAQGYDRFQAKSKTALEVIQDRIRWSGAEKTRVVEALEAA
                     LRVGQGKVDVHVEDGPVWRFSADLHCADCDIHYAEPTPSLFSFNSPLGACETCRGFGR
                     VIGIDFGLVVPDEAKTLREGAVRCWQSPSFKECQDDLETYAKKRGIPLDVPFRDLSEK
                     QRQWVLEGELDWVSWRKSWPGVWYGVRRFFTWLETKAYKMHVRVLLSRYRAYNPCGDC
                     GGARLKPEALLWRLGARAPRQARGDRETGLGIHEMMLLPISKVRGFFDSLQLPAPLDE
                     ATDLLLTEIRARLGYLEEVGLGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVL
                     DEPSIGLHPRDMGRVIAVMRRLRDTGNSLVVVEHDPQIMFAADRLLDMGPGPGERGGE
                     VVFFGTPAEMRSARTLTADYLSGRNRADGAMVHPPRRDGAALKLRGATEHNLKGIDVA
                     FPLGQLVCVTGVSGSGKSTLVQDVLYPALRKAKGKPSETPGAFAALEGAGQIEDVVMV
                     DQTPIGRTTRSNPASYVGAWDAIRAIWAAHPLAKERKYTAGTFSFNSGNGRCPACGGN
                     GFEHVEMQFLSDVYLRCPACDGKRFRAEVLEIEVEGKSIADVLELTVSQALIFFSDRA
                     EVKRALQPLSDVGLEYLRLGQPVPTLSGGEAQRLKLAGHLVEAARGSTGSPRTDAGKL
                     FLFDEPTTGLHFDDVARLLRAFRQLIAAGHSLVVIEHNLDVIRGSDWIVDLGPEGGDA
                     GGGIVCQGTPDVVAAHASSHTGRALREYETALATPPALRPSASSGAHGERPQHRPPNA
                     ITIHNAREHNLRIDDLQIPRDKFTVITGVSGSGKSTLAFDILFSEGQRRYLESLNAYA
                     RQFVQPSARPDVDAIFGSPPTVAIEQR"
BASE COUNT          902 a         2172 c         2067 g          849 t
ORIGIN      
        1 attttcgcag gcgccgcgta gcccggatgc cggcccgccg cgcggccctc agccgccccg
       61 ccgcctcccg gcccggtccg cccgcgccgc ctcgcgggcg cgctcgatga agagcttggc
      121 gacggggctg agcgcgcggc ccttcagggt gacgatcacg ttttgcaccc gggccctcgg
      181 cagagcgacg ggcaggatcc ggatctccgg gcgcagcgtc gggaacctcg agatcgaggc
      241 cgggaagatc gtgacgaagc gcccggtccc gaggaggctg atgcgcgtct cgggagagtc
      301 gcagaccacg gtcgtgcggg gatagtcgag tcctgcagcg cgaaaggcct ccatcgcgat
      361 cgacgcgatc gcgcttcccg gcggcggcag cgtccacgct tcgcccgcca gctccgccag
      421 ctcgatccgg cgccgccgca cccaccggct cttcgcgccg gccgcgacga catacgagtc
      481 gtcgaagaga ggctcgaact cgagctgctc ctccgcggcg ggagcgaatc tccgcacgac
      541 gaggaaatcg acctcgcgcg cgagcagcgc gcggcgcagc ggctccgcgt aggccgtcac
      601 caggtgaaag accatgcgcg gatagcgccg cgacagccgg tcgaccaccg cggagacgaa
      661 gctcgcggcg agaatgggcg tgcacccgat cctcacctcc ccggccgcgg gatcggcgag
      721 aaactcgacg ttgcgcaccg cccggcgcag ctcgtcgaag accgccacgc cgccctcgag
      781 cacggcgcgg ccgtacgcgg tcggctccac gccccggacg ctgcgctcga gcagccgcac
      841 gccgagcgcg ccctccagct cggcgatgga ccgggacacg gccggctggg tggtgtggag
      901 gagcgccgcc gccttgctca tgctgcgggc ctgcgccacg gtcatgagca cgtgcaggtc
      961 gcgcagcttc atccgcagcc cggctcgatc gcccggcggc acggcgttac ccattgcagg
     1021 tccgatatgg gagcatcaga agacggcatt atgtgcgcat ggccgcctcc acgtatattc
     1081 gccagggacc cgaggagaac gccatgacat tttcgcgccg ccgattcctg aatctcgcag
     1141 ccggggctgc cgcgctcgcg gccgtgccgc gcctcgcgcg ggcgcaggcc tacccgtcgc
     1201 ggccggtgcg catcgtcgtc ggcttctccg ccggcggccc gaacgacgtg ctcgcgcgcc
     1261 tgatcggcga gcggctgtcg cagcggctcg gccagccctt cgtggtcgag aaccggccgg
     1321 gtgccggcgg caatatcgcc accgaggcgg tggtgcgcgc gcccgcggac ggctacacgc
     1381 tcctcctggt cggctcgccg aacgcgatca acgcgacgct ctacgacaag ctcggcttcg
     1441 atttcatccg cgacatcgcg ccggttgccg gcctgatgcg cggcgacctc gtcatggtgg
     1501 tgcatccgtc ggtcccggcc gcgacgcttc ccgagctcat cgcctacgcg aaggcacatc
     1561 cgggcaagct ttcctacggc tcggccggcg tcggcgggat cacccatatc accgccgagc
     1621 tgttcaagat gatggccggc gtcgacatcg tccacgtgcc gtaccgcggc gtcgcgcccg
     1681 cgctgaccga cctgctcgcc ggcgaggtgc aggtcgtgtt caccaacccg ggccagaccg
     1741 ttcaatacac ccgggccggc aggctgcgcg cgctcgcgat caccacggcg aagcgctcga
     1801 gcgcgtttcc ggacatcccg accgtgggcg aatccgtccc gggctacgag gcgggctcgc
     1861 tcttcggcat cggcgcgccg aagaacacgc ccgcggagat cgtcgaaaag ctcaacgggg
     1921 agatcgacgc cgcgcttgcc gatcccgggt tcaggacgcg gctcgccaag ctcgacggca
     1981 cggtgctcgg cggctcgccc gccgatttcg gccggctcgt cgccgccgaa accgagaagt
     2041 ggggcaaggt cataaagttt gcgaaaatca ggcccgactg acctggaggc tgcgacgggc
     2101 tttctcaaca tccgatcgag acacggcggc gagttcgcgt gctacctggc cgcgcccgcg
     2161 atcggcgggc cggtcccggc cgtcgtgctc gcctccgccg tgcacggcgt cgacgcggac
     2221 ctgcgcgcca tcgcggacga ctttgccgcg cacggcttca tcgccgcggc gcccgacctg
     2281 ttctggcgct cgatcccggg gccgctttcc cgcgacgacg agcgcaccgc gcagcgctcg
     2341 cggcctcgcc tggaaaaaat caggatcggc gaaagcgaca tggccgacac cctcgcgtac
     2401 ctcggcacgc tgcccaattt caacgggcgc gccgcggcga tggggttctg ctacggcggc
     2461 ccgtacgcga tcctcgggcc gaagcgcctc ggctacgccg cgggcatttc ctgccacggc
     2521 agccagatgc tggactacat cgcagagctc gacggcgtcg gcgcgccggt ctgcatcctc
     2581 tggggcgacc gcgaccacca ggcgccggcc ccggtgctgg aggcctatcg cgccctggcg
     2641 tcgcgccgga agaacgtgga ggtgcacgtc tttcccggcg tgctccacgg gtacatgatg
     2701 cccggggcgc aggcgttcca tccggcgacg cgcgagctct cgatgcagcg cgcgcgggcg
     2761 atcctcgacg gtctcaaagg cgctccgcct cggggttgac acgcccgcta caatagcggg
     2821 atgcagcacc gcaagtccgg cgcggcgatc acgatccgcg gcgcccggca aaacaacctc
     2881 aagaacctga ccctcgagat cccgacgaac gagctggtgg tcgtcaccgg cgtctcgggc
     2941 tcggggaagt cgtcgctcgt cttcgacacg ctgtacgccg aagggcagcg tcgctacgtc
     3001 gagacgttct cgccgtacgc gcgccagttc ctcgaccgca tggacaagcc gcaggtcgac
     3061 gccatcgagg gcatcccgcc ggccatcgcc atcgaccaga cgaacccggt gcgcacgtcg
     3121 cgctcgaccg tcggcacgat gaccgagctg aacgaccacc tgaagctgct ctacgcgcgc
     3181 gccgccgggc tttactgccg cggctgcggg cgggcggtgc ggcgcgacac gccggagtcg
     3241 atctacgccg agatcgcggc gcgcgcagcg aaagaaggcg acccgcgcct gatcgtcgcc
     3301 ttccccgtcc cgatcccgaa gaacttcaag gccgacgaga tcgaggcggc gctgaaggct
     3361 caagggtacg accggttcca ggcgaagtcg aagaccgcgc tcgaggtgat ccaggatcgt
     3421 atacggtggt cgggggcgga gaagacgcgc gtggtcgagg cgctcgaggc ggcgctgcgc
     3481 gtcggccagg gcaaggtcga cgtccacgtc gaggacggcc cggtgtggcg cttctccgcc
     3541 gacctgcact gcgccgactg cgacatccac tacgccgagc cgacgccgtc gctgttctcg
     3601 ttcaactcgc cgctcggcgc gtgcgagacc tgccgcggct tcggacgcgt gatcgggatc
     3661 gacttcgggc tcgtcgtgcc cgacgaggcg aagacgctgc gcgagggcgc ggtgcgctgc
     3721 tggcagtcgc cctcgttcaa ggagtgccag gacgacctcg agacatacgc caagaagcgc
     3781 ggcatcccgc tcgacgtccc gttccgcgat ctctccgaga agcagcgcca gtgggtgctc
     3841 gaaggcgagc tcgactgggt gagctggagg aagtcgtggc ccggcgtctg gtacggcgtg
     3901 cgccgcttct tcacctggct cgagacgaag gcgtacaaga tgcacgtgcg cgtgctgctg
     3961 tcgcgctacc gcgcgtacaa cccgtgcggg gattgcggcg gggcgcggct gaagccggag
     4021 gcgctgctgt ggcggctggg ggctcgggcc cctcgacaag ctcggggtga tcgggagacg
     4081 ggcctcggca tccacgagat gatgctgctg ccgatctcga aggtgcgcgg cttcttcgac
     4141 tcgctccagc ttcccgcccc gctcgacgag gcgaccgacc tgctgctgac cgagatccgc
     4201 gcgcgcctcg gctacctcga ggaggtcggc ctcggctacc tcacgctcga ccggcagtcg
     4261 cgcacgctct cgggcggcga ggtgcagcgc atcaacctca ccaccgcgct cggcacctcg
     4321 ctcgtcaaca cgctgttcgt gctcgacgag ccctcgatcg gcctgcaccc gcgcgacatg
     4381 gggcgcgtca tcgccgtgat gcgccggctg cgcgacaccg gcaactcgct cgtcgtggtc
     4441 gagcacgacc cgcagatcat gttcgccgcc gaccgcctgc tcgacatggg gcccggtccc
     4501 ggcgagcgcg gcggcgaggt ggtgttcttc ggcacgcccg ccgagatgcg cagcgcgcgc
     4561 acgctcacgg ccgactacct ctcgggccgc aaccgcgccg acggcgcgat ggtgcacccg
     4621 ccgcggcgcg acggcgcggc gctgaagctg cgcggcgcga ccgagcacaa cctcaagggc
     4681 atcgacgtcg ccttcccgct cgggcagctc gtgtgcgtca ccggcgtctc cggctcgggc
     4741 aagtcgaccc tggtgcagga cgtgctctac cccgccttgc gcaaggcgaa gggcaagccg
     4801 agcgagacgc cgggcgcctt cgccgcgctc gagggcgccg ggcagatcga ggacgtcgtg
     4861 atggtcgacc agacgccgat cggccgcacc acgcgctcca accccgcgag ctacgtcggc
     4921 gcgtgggacg cgatccgcgc gatctgggcc gcgcacccgc tcgcgaagga gcgcaagtac
     4981 accgccggca ccttcagctt caactcgggc aacggccgct gcccggcctg cggcggcaac
     5041 ggcttcgagc acgtcgagat gcagttcctc tccgacgtgt atctccgctg ccccgcgtgc
     5101 gacggcaagc gcttccgcgc cgaggttctc gagatcgagg tcgagggcaa gtcgatcgcc
     5161 gacgtgctcg agctgaccgt gtcgcaggcg ctcatctttt tctccgaccg ggcggaagta
     5221 aagcgcgcgt tgcagccgct ctcggacgtc gggctcgagt acctgcggct ggggcagccg
     5281 gtgccgacgc tgtcgggtgg ggaagcgcag agattgaaac tggcggggca cctggtggaa
     5341 gcggctcgcg gttcgacagg ctcaccgcga acggacgcgg gcaagctgtt cctgttcgac
     5401 gagccgacca ccggcctgca cttcgacgac gtcgcgcggc tgctgcgcgc gttccggcag
     5461 ctcatcgccg cgggacactc gctcgtcgtc atcgagcaca acctcgacgt gatccgcggc
     5521 tccgactgga tcgtcgacct cggccccgag ggcggcgacg cgggcggcgg gatcgtgtgc
     5581 cagggcacgc ccgacgtcgt cgcggcgcac gcgagctcgc acacggggcg ggcgctgcgc
     5641 gagtacgaga cggcgctcgc gacgcccccg gcccttcgcc cttcggcaag ctcgggcgct
     5701 cacggcgaac ggccgcagca caggccgccg aacgcgatca cgatccacaa tgcgcgcgag
     5761 cacaacctga gaatcgacga tctccagatc ccgcgcgaca agttcacggt catcaccggc
     5821 gtctcgggct cgggcaagtc gacgctcgcc ttcgacatcc tgttctcgga aggacagcgc
     5881 cggtatctcg agtctctgaa cgcgtacgcg cgccagttcg tgcagccctc ggcgcggccc
     5941 gacgtggacg cgatcttcgg cagtccgccg acggtcgcga tcgagcagcg
//
LOCUS       DATDMW010000017         3604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_500807, whole genome shotgun sequence.
ACCESSION   DATDMW010000017 DATDMW010000000
VERSION     DATDMW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500807"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1416)
                     /locus_tag="VI319_00495"
     CDS             complement(28..1416)
                     /locus_tag="VI319_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6862352.1"
                     /translation="MKTFSLALRNLLRNRRRSAMTLFAMAIGALTILVFGGYVRNILY
                     SLQTGYVLRGGHIQIQRKDYFLYGTGNPAAYGIKDYPRVIDVVVHDPVLAPMTRVVTP
                     SISLGGIAGNFDAGVSRTVSGVGLVVDDRMRMREWNDYRLPLNLPPFALKGSAPDAAV
                     VGIGVARVLELCQPLGIPDCVRKAPLQEAPGKRAPEDIAALAAAESRPTASPGEAHIE
                     LLAASAHGAPNVAALRVIKAERQGFKEIDDMFVGMHLAQAQRLLYGKDKPEATAVVIQ
                     LQHTSQIPAARARINELLATTLKGEPLEVQDFEALNPFYGQAIGMFGAIFGFMAVLIG
                     AIVLFTVGNTMSMAVVERTTEIGTLRAIGLRRSGIRNLFMCEGLLLGLIGTAAGMALA
                     LALAWLINHSGLTWLPPGNVDRVPLSVRVWGETALLLFTGIGLTAVAVVSAWWPARRA
                     ARLPVVDALRHV"
     gene            complement(1413..2114)
                     /locus_tag="VI319_00500"
     CDS             complement(1413..2114)
                     /locus_tag="VI319_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862353.1"
                     /translation="MDVVSLRGIAKTYHLDAVDVPALADVDLDIRADCFTVISGPSGS
                     GKTTLLNIVGCIDQPDRGDVRVAGEDVRALSDDALSDFRARHLGFVFQNFNLLPVLTV
                     YENVEYPLLLSRQPAARRRRRVEALLEAVGLADKARNLPGQLSGGQRQRVAIARALAS
                     SPKLVLADEPTANLDSQTGASIISLMRDMQREQHVSFVFSSHDPKVLAAADDAIVIRD
                     GRIEKVERRPAGGAA"
     gene            complement(2132..2638)
                     /locus_tag="VI319_00505"
     CDS             complement(2132..2638)
                     /locus_tag="VI319_00505"
                     /inference="COORDINATES: protein motif:HMM:NF042671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HEY6862354.1"
                     /translation="MNIASILHGTPGWVFGLFAVLVVLGLQALRTRSFAPWRLLVVPA
                     VFIGWGLATLAVRSAASPALAAAWAGGALTGIAVGWLATRPDAVRFDADSARVTVPGS
                     AATLLRNMSIFGAKYGLAVAMARAPEHAFALLAWDGGVSGLAAGYFFGWLLRVGAQYR
                     AARLQPAT"
     gene            2638..3336
                     /locus_tag="VI319_00510"
     CDS             2638..3336
                     /locus_tag="VI319_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY6862355.1"
                     /translation="MRASALRRYHAVPMPRTLRYPSVPRQVPPPGPDEWGPALRDRVI
                     GAAFHAFTEKGYSRTTTLDIASRAKVSKRELYAHFGSKQDLLVACIASRTSRMRPPDL
                     PEPQDRAMLERSLTTFAETLLREVSHPAVLAMFRLAIGEATVSPQVARTLETRGRSRG
                     RSALAKLIGRAQAKGLLGKGQAEDLARDYLGLLWQDLLVGLLLDVAKPPSQREIARRA
                     ARATASFMKLARAE"
     gene            complement(3338..>3604)
                     /locus_tag="VI319_00515"
     CDS             complement(3338..>3604)
                     /locus_tag="VI319_00515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6862356.1"
                     /translation="VERVVGKLIDALRRPYPGIGDTQRRVSATLGVSVFPDDATDGAT
                     LMENADEAMYTAKQAGKNAYCLYSSTRKPAPPNGAALANSGSGS"
BASE COUNT          551 a         1256 c         1313 g          484 t
ORIGIN      
        1 agggcagcga ggaagccgga gccgcgctca gacatggcgc agcgcgtcca cgaccggcag
       61 gcgcgcggcg cggcgcgccg gccaccacgc cgacacgacg gcgaccgcgg tgaggcctat
      121 gccagtgaag aggagaagcg cggtctcgcc ccacacgcgc acgctgaggg gcacgcgatc
      181 gacgttgccc ggcggcagcc acgtgagccc ggaatgattg atgagccagg cgagggcgag
      241 cgcgagcgcc atcccggccg cggtgccgat caggccgagc agcagccctt cgcacatgaa
      301 gaggttgcgg atcccggagc ggcgcaggcc gatggcgcgc agcgtgccga tctcggtggt
      361 gcgctcgacc accgccatgc tcatcgtgtt gcccacggtg aagagcacga tcgcgccgat
      421 caaaaccgcc atgaagccga agatcgcgcc gaacatgccg atcgcctggc cgtagaacgg
      481 gttcagcgcc tcgaagtcct gcacctccag gggctcgccc ttgagcgtgg tcgccagcag
      541 ctcgttgatg cgcgcccgcg ccgccgggat ctggctcgtg tgctgcagct ggatcaccac
      601 ggcggtcgcc tccggcttgt ccttgccgta gagcaggcgc tgcgcctgcg cgaggtgcat
      661 gccgacgaac atgtcgtcga tctccttgaa cccctggcgc tcggccttga tcacgcgcag
      721 cgcggcgaca ttcggcgcgc cgtgcgcgct ggcggcgagc agctcgatgt gcgcctcgcc
      781 gggcgacgcc gtcggccgcg actccgcggc cgcgagcgcg gcgatgtcct cgggcgcgcg
      841 cttgccgggc gcttcctgga gcggcgcctt gcgcacgcag tccgggatgc cgagcggctg
      901 gcagagctcc agcacccgcg cgacgccgat gccgaccacc gcggcgtccg gcgcgctgcc
      961 cttcagcgcg aagggcggca ggttgagcgg caggcggtag tcgttccact cgcgcatgcg
     1021 catgcgatcg tcgaccacca ggcccacgcc gctcaccgtg cgcgatacgc cggcgtcgaa
     1081 attgccggcg atgccgccga gcgagatcga cggcgtgacg accctcgtca tcggcgcgag
     1141 gaccgggtcg tgcaccacga cgtcgatgac gcgcggatag tccttgatgc cgtaggcggc
     1201 gggattgccg gtgccgtaga ggaagtagtc cttgcgctgg atctggatgt ggccgccgcg
     1261 cagcacgtag cccgtctgca gcgaatacag gatgttgcgc acgtagccgc cgaagacgag
     1321 gatcgtcagc gcgcctatcg ccatggcgaa cagcgtcatc gccgaacgcc ggcggttgcg
     1381 cagcaggttg cgcagcgcga gcgagaaggt cttcatgccg cgcctcccgc ggggcggcgc
     1441 tcgaccttct cgatgcggcc gtcgcggatg acgatcgcgt cgtcggcggc cgcgagcacc
     1501 ttgggatcgt gcgacgagaa gacgaacgac acgtgctgct cgcgctgcat gtcgcgcatg
     1561 agcgagatga tcgacgcgcc ggtctggctg tcgaggttcg ccgtcggctc gtcggcgagc
     1621 acgagcttcg gcgaggaggc gagcgcgcgc gcgatggcga cgcgctggcg ctggccgccg
     1681 gagagctgcc cgggcaggtt gcgcgccttg tcggcgaggc cgaccgcctc gagcagcgcc
     1741 tcgacgcggc ggcgcctgcg cgccgccggc tgacgcgaga ggagcagcgg atactccacg
     1801 ttctcgtaca cggtgagcac cggcagcagg ttgaagttct ggaagacgaa gccgaggtgg
     1861 cgcgcccgga aatcggacag cgcgtcgtcg gagagcgcgc gcacgtcctc gcccgcgacg
     1921 cgcacgtcgc cgcgatcggg ctggtcgatg cacccgacga tgttgaggag cgtcgtcttg
     1981 ccgctgcccg agggcccgga gatgacggtg aagcagtcgg cgcggatgtc gaggtccacg
     2041 tcggcgagcg ccggcacgtc gaccgcgtcg aggtggtagg tcttcgcgat gccgcgcagc
     2101 gatacgacgt ccatgcttgc ctcccgggct gctatgttgc cggctggagg cgcgccgcgc
     2161 ggtactgcgc gccgactcgc agcagccagc cgaagaagta tccggccgca aggcccgaca
     2221 cgccgccgtc ccaggcgagg agcgcgaacg cgtgctcggg ggcgcgcgcc atcgccaccg
     2281 cgaggccgta cttggccccg aatatcgaca tgttacggag gagggtcgcc gcgctgcccg
     2341 gcacggtgac ccgggcgctg tccgcgtcga agcgcacggc gtccggccgc gtggcgagcc
     2401 agccgacggc gatgccggtg agcgcgccgc cggcccaggc tgcggcgagc gcgggggagg
     2461 cagcggagcg cacggcgagc gtggcgagcc cccagccgat gaatacggcc gggacgacga
     2521 gcagccgcca tggggcgaag ctgcgggtgc gcagcgcctg caggccgagg accacgagga
     2581 ccgcgaagag cccgaacacc cagcccgggg tgccgtgaag gatcgaagcg atgttcattg
     2641 cgcgcctccg cgctgcggcg gtaccatgcg gtaccaatgc cgcgcactct acggtaccca
     2701 tcagtaccac gtcaagtccc gcctccgggg ccggacgaat gggggcctgc gctgcgcgac
     2761 cgggtcatcg gcgccgcctt ccacgccttc accgagaagg gctattcgcg gaccaccacg
     2821 ctcgacatag cgagccgcgc caaggtctcg aagcgcgagc tctacgcgca cttcggcagc
     2881 aagcaggacc tgctcgtcgc ttgcatcgcc tcgcgcactt cgcgcatgag gccgccggac
     2941 ctgcccgagc cgcaggaccg cgccatgctc gagcgctcgc tcaccacgtt cgccgaaacg
     3001 ctgctgcgcg aagtgagcca cccggccgtg ctcgccatgt tccgcctcgc catcggcgaa
     3061 gcgacggtct cgcctcaggt ggcccgcacg ctggaaaccc ggggccggag ccggggccga
     3121 agcgccctcg cgaaactgat cgggcgcgcc caggcgaagg gcctgctcgg caagggacag
     3181 gccgaggatc tcgcccgcga ctacctcggc ctgctctggc aggacctcct ggtcggcctt
     3241 ctcctcgacg tcgcgaagcc gccgtcgcag cgcgagatcg ctcgccgtgc ggcacgcgcg
     3301 accgccagct tcatgaagct ggcgcgcgcg gaatgaatta cgaccctgac cccgaattcg
     3361 cgagcgcggc gccgttcggc ggcgcgggct tgcgggtgct cgagtagagg caataggcgt
     3421 tcttgccggc ctgcttggcg gtatacatcg cctcgtcggc gttctccatg agcgtcgcgc
     3481 cgtcggtggc gtcgtcgggg aagacgctga cgccgagcgt ggcggagacg cgccgctgcg
     3541 tgtcgccgat acccgggtag ggccggcgca gcgcgtcgat cagcttgccg acgacgcgct
     3601 ccac
//
LOCUS       DATDMW010000018        11804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1994533, whole genome shotgun sequence.
ACCESSION   DATDMW010000018 DATDMW010000000
VERSION     DATDMW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994533"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VI319_00520"
     CDS             <1..662
                     /locus_tag="VI319_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY6862357.1"
                     /translation="RLALKQNLTGVSVGPLLKDLMGQDRLEGRGNVSLDVTSQGDTVS
                     AIKKALGGSARLDLRDGSIRGVNIAQAIRGAKAALGRGGGEGAGKTDEKTDFSELSAS
                     FRIANGVAHNDDLALKSPLFRVGGAGDVDIGREQLDYTVKATIVSSLQGQGGPELEAL
                     KGVTVPVRLSGPFDRIGYKVDLGSVVTEKAKERLNERRDEIRQQAEDKLKDRLKGLLQ
                     R"
     gene            complement(659..2674)
                     /locus_tag="VI319_00525"
     CDS             complement(659..2674)
                     /locus_tag="VI319_00525"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6862358.1"
                     /translation="MSESAGLLEQAVLEHRAGRLDAAARLYRQVVDAEPRNADALRLL
                     GLLELQQGRTESAIARLERAAQAAGKSAPVLNDLGKAYFAAGRPADAKKCFARALSIK
                     ADDAEVHGNLGAVLAELNDLRGAAASLKRAVALQPAYPNAHYNLGQVLQMMGEPREAE
                     RAYRAALALDPGMVDAHYNVVNIFNLLHVQGRVEQGEQAFRRALERDPEDAQARWALG
                     VSRMCTLASASDAGPSAAATELRALSDWVASRAIAHPERVVASVQPFYLAYHDGNHRE
                     LLAQYGRLCADTMRGWLERERIGAPAAGERSGRLRVGIVSAQVQRHSTWAAFAQGWVE
                     RLDRERIELSVFHLGRTHDDETGRAAALAAHFEHGPMRSPREWVDAILRRRPEVLIYP
                     APAQDSQEMRLASLRLAPVQAAAWGHPETTGLPTIDAYLSAEAFEPGDADAHYSERLV
                     RLPRLGTCYARRAPAAAEPDWRSLGFDPEAPLLLCVGSPFKYPPAFDPVFPEIARRAP
                     GARLVFFENEPRFLSAGLEQRLRAAFARAGLAFERHVVFLPRLAHGEFYGLMRRAAAW
                     LDTPVYSGFNTAMQALECALPIVAHEGRFMRGRFASGILRQMRMEEWIARDDARFAEL
                     AALLATDAARRRDASERITAARDALFDDAAPVRALEEFLFAAARPGR"
     gene            complement(2671..3051)
                     /locus_tag="VI319_00530"
     CDS             complement(2671..3051)
                     /locus_tag="VI319_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY6862359.1"
                     /translation="MAATAKIFLVLGSLAALAAVLLGAFGAHALKARLDADALALWRT
                     AVEYHFWHALGLVAVGLVAAHLPGSVLLKWSGWAMLAGIVLFSGSLYLLALTGVRALG
                     AVTPVGGVAFLAAWALLAAAVLRA"
     gene            3102..3563
                     /locus_tag="VI319_00535"
     CDS             3102..3563
                     /locus_tag="VI319_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY6862360.1"
                     /translation="MPAVVIPSIEALKRLVGTEVAVSDWFALTQDRIREFADATGDHQ
                     WIHVDEARAAKESPYRGTVAHGFLTLSLLPHLLGQAIDLRGARMGVNYGLNRARFTGP
                     VPAGSRVRARFALAACEDIPNGVQTTWTVTVEREGDAKPVLVAEWLTRRYA"
     gene            complement(3570..4271)
                     /locus_tag="VI319_00540"
     CDS             complement(3570..4271)
                     /locus_tag="VI319_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY6862361.1"
                     /translation="MDRCPQRRKLLRLAGASGAALVALGAPSRAAEPLDPAGPAPTPV
                     EDLIHAQGLLERVLLVYEESARRLAGADEPVQPEVVRDAAQIVRGYVEDHHQKLEEKH
                     VFPRFERAAAQEHVDLVRVLRKQHEAGRRLTDRILKLAGTTLDDPVARNQLQTSLRDA
                     VRMLRPHESREDTVLFPAFRRMVGAAEYRALGEAMKSAERRAPAGGLEMMVEKVSALE
                     RQLGLYDLSKFTPKA"
     gene            4478..5476
                     /locus_tag="VI319_00545"
     CDS             4478..5476
                     /locus_tag="VI319_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862362.1"
                     /translation="MTAFAAGLAAALLAAPLTAAAQAKLSVGMPTNPPNIIHTPVFIA
                     DDLGYYKADGLKVEFLSFEGGVYSFRAMISGDVDIAAASGSFALVARSNGAPSKLIFS
                     SAPKLESVMMAQGDIKSVKELKGRKIGIQEPGGFAFVLSMTVLGSAGLKKDDVEMVSI
                     LSEDVPPLVAGQIDTAILHVDQEIVAKEKKPSLHPLARLSEVAPRQLYLAYAVKEDTI
                     KKKRKALVAFTKDNIRAVRMIYSDRKKVEPIIREHTGLDAPVVSKALDVLIKNCAWDA
                     NSGLPRDMIDYTVERMVKVGNIDKGKGTTYDQAVDLSIANEALKELGPWKGPTCAH"
     gene            5504..6325
                     /locus_tag="VI319_00550"
     CDS             5504..6325
                     /locus_tag="VI319_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6862363.1"
                     /translation="MSKPAAPAVPAASAPRERAPGVPLRSRPAVYRIASVIVGLAVWE
                     IVGPHIDPIFFTYPSAIARAAWEMTASGELPKYAWESLQILFYGMLLAIGVGIPVGVI
                     MARFRKVDWVLDTWLNALYSTPTVALVPLLVLWFGIRAEAKVVVVFLFAIFPVLINTY
                     QGVRKVDASFLEVARSFRSSEWQMWRDVLLPWAVPYITAGIRLAIGRGLVGMIIAEFY
                     TAMTGLGYMIVRYAHIFKMDRTFVPVILLMFLGIALTAALKWVERRIAPWNRTED"
     gene            6327..7103
                     /locus_tag="VI319_00555"
     CDS             6327..7103
                     /locus_tag="VI319_00555"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862364.1"
                     /translation="MADRIALKRVGKRYGALQVCRNIDLTVGEREIVTIVGPSGCGKT
                     TLLRCIDGLIPYEDGDILVEGERITEPRAGVAMVFQHFGLFPWKTVYDNVAYGLRIRG
                     EPAERIAAAVPEYVNMVGLSGFERHYPYQLSGGMQQRCGLARALAVEPKVLLMDEPFA
                     AVDAQTREILQFELLRIWEQRPTTMIFVTHSIEEAVLMGDRVVILKGRPSGVHEVVDV
                     GIPHPRSRATLAERRFAEVREHVWSALMDEARKAEYELGR"
     gene            7108..7425
                     /locus_tag="VI319_00560"
     CDS             7108..7425
                     /locus_tag="VI319_00560"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HEY6862365.1"
                     /translation="MGEAAFALAVAALAWLWYDSMRARERAVAAGRVACERDGLQLLD
                     DTVECVSVRPARALSGHARLRRIYRFEFSDTGDNRRAGRIVMLGAEVESLTMDPFLLR
                     HPQ"
     gene            7450..8193
                     /locus_tag="VI319_00565"
     CDS             7450..8193
                     /locus_tag="VI319_00565"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEY6862366.1"
                     /translation="MSRWVEGTVAGQKRWTERLYSLSVNADVKSFEAGQFTKLALAVG
                     GEMVARPYSFVNGPKERPHEFYYIVLPEGPLTQRLSQLETGDNVYLAPQPSGFLVLSE
                     VPDAEILWLISSGTGVGPFLSILATEAPWQRYRQVVLVQAVRQPEELTYRDRIESLSS
                     AHVGQMRHVPFISREHRPPALHGRVPSAIDDGRLEAAAGVPLSAQTSQVMICGNPEMI
                     TDTTEALKRRGMRKHRRREPGHITVENYW"
     gene            8190..9050
                     /locus_tag="VI319_00570"
     CDS             8190..9050
                     /locus_tag="VI319_00570"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HEY6862367.1"
                     /translation="MRIPASIAIAAALLLAGCAGMTRIAYNNADFFLRKMANDYFALD
                     DAQADQLRVRLRRFHAWHRANELPQYVAFLRTAQRRFTKGLTPEDVAWGMSTVRARFR
                     AFAAKAAEEAAPMLSSLAPEQIGVLERRLAENNAKYVRENLPPDERKRIRVQTRRMAD
                     ALSQWAGDLTRDQEARVERFVRAHDRFAMMRFEDRVRGQREAVALLRKRLPPQELAPL
                     LARHFNDPDVRRSDEFRREEKRWEADFGRLLAELSGTLSPEQRAHVIDNIERFADDFA
                     ILAGDARNAA"
     gene            9047..9613
                     /locus_tag="VI319_00575"
     CDS             9047..9613
                     /locus_tag="VI319_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY6862368.1"
                     /translation="MKVRAVALVVLLLLIAGARAAELKPWTGDATPPALALKDLEGRE
                     HRLADYRGKVVLVNFWATWCEPCREEMPAMQRLQQRLEGKPFVVLAVDFGEGDARVKG
                     FLKELPLPFTFLLDRDSSVARAWRVRVLPVSFLIDADQRIRYSAVGDAAWDSPAVEQV
                     VRRLLPRAALRRAGHNAGIARSGDHPSP"
     gene            9629..10732
                     /locus_tag="VI319_00580"
     CDS             9629..10732
                     /locus_tag="VI319_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEY6862369.1"
                     /translation="MDRRSFLKSGVLLPAAAAPRFATAQQLPFQPRQAQSWRMFEVTT
                     RVDVLVPGGTTKVWLPVPSVDSTYQKVIDNTWSGNASTAKLVHDGKYGAGMLYAEWPD
                     AENKPYVELHSRFMTRDRAVDFGSRLTRVGDRLTPEERRFYTAPTDHIPVDGIVLQTA
                     RDVTKGTKTDHDKAKAIYEWIVDNTFRDPKVRGCGTGDIKAMLETGNLGGKCADLNAL
                     YVGLARASGLPARDVYGVRVAKSQFGYRSLGAGTANITRAQHCRAEVYLEAWGWVPVD
                     PADVRKVVLEEKAQPTTVDDPLVQAVRPKLFGAWEMNWLAFNTANDIALPNSQYAKLT
                     FLMYPQAETAAGRLDSLDPDNFRYSMSSKELTA"
     gene            10747..11523
                     /locus_tag="VI319_00585"
     CDS             10747..11523
                     /locus_tag="VI319_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6862370.1"
                     /translation="MHFSHANGFPAPSYRKFFAGLERDFEIGWIERIGHDPRYPVTDG
                     WPHLVDELIGHVESTYRDPVVAAGHSLGGFLTLRAAARRPDLFRAIVVMDSPIPKRWS
                     GTALQMVKRLGLVDRVTPAGTTRGRRSEWSSAEEAVEHFRGKRNFLRFDPECLRDYVT
                     LGMRRDGRGVRLAFDPGIEYRIYRTIPHDLARDLPALKVPAGFVCGEESSEMRRPGLV
                     DPRRHFRVARVPGGHLFPFERPAEAAAAVGALALDLAMPA"
     gene            complement(11528..>11804)
                     /locus_tag="VI319_00590"
     CDS             complement(11528..>11804)
                     /locus_tag="VI319_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6862371.1"
                     /translation="TCTSDAAEALRQAACLRPEIVILDIGMPTMDGYEVLRRLRAFPA
                     AASMTIIALTGFAQERDAAHAIAEGFDHHFAKPLDFGRLAALLEGRG"
BASE COUNT         1769 a         4197 c         4183 g         1655 t
ORIGIN      
        1 tgcggctcgc gctgaagcag aacctgaccg gcgtcagcgt cggcccgctg ctcaaggacc
       61 tgatgggcca ggaccggctc gagggccgcg gcaacgtgtc gctcgacgtc acgtcgcagg
      121 gcgacaccgt gtcggcgatc aagaaggcgc tcggcggcag cgcgcgcctg gatctgcgcg
      181 acggctcgat ccgcggcgtc aacatcgcgc aggcgatccg cggcgcgaag gcggcgctcg
      241 gccgcggcgg cggcgagggc gcgggcaaga ccgacgagaa gaccgacttc agcgagctgt
      301 cggcgagctt cagaatcgcg aacggcgtcg cgcacaacga cgacctcgcg ctcaaatcgc
      361 cgctgttccg cgtcggcggc gcgggcgacg tcgacatcgg ccgcgagcag ctcgattaca
      421 ccgtgaaggc gacgatcgtc tcgtcgctgc aggggcaggg cgggccggaa ctcgaggcgc
      481 tgaagggcgt caccgtgccg gtgcggctct cgggaccgtt cgacaggatc ggctacaagg
      541 tcgacctggg ctcggttgtg accgagaagg cgaaggagcg gttgaacgag cgtcgcgacg
      601 agatccggca gcaggccgag gacaagctga aggaccgcct caaggggctg ctgcagcgct
      661 agcgccccgg acgcgccgcg gcgaagagga actcctcgag cgccctgacc ggtgccgcgt
      721 cgtcgaacag cgcgtcgcgc gccgcggtga tgcgctcgct cgcgtcgcgg cggcgcgccg
      781 cgtcggtggc gagcagcgcc gcgagctcgg cgaagcgcgc gtcgtcgcgg gcgatccatt
      841 cctccatccg catctgccgc aggatgccgc tcgcgaagcg gccgcgcatg aagcggccct
      901 cgtgcgcgac gatcggcagg gcgcactcga gcgcctgcat cgcggtgttg aacccggagt
      961 acacgggcgt gtcgagccat gcggccgcgc gacgcatcag gccgtagaac tcgccgtgcg
     1021 cgaggcgcgg gaggaaaacg acgtgccgct cgaaggcgag ccctgcgcgc gcgaacgccg
     1081 cgcgcaggcg ctgctcgagc ccggcggaga gaaaccgcgg ctcgttctcg aagaagacga
     1141 gccgcgcgcc gggcgcgcgc cgagcgatct cggggaacac cgggtcgaac gccggcggat
     1201 acttgaaggg gctgccgacg caaagcagga gcggcgcttc aggatcgaat ccgagcgagc
     1261 gccagtcggg ctcggcggcc gcgggcgcgc gccgcgcgta gcacgtgcca aggcgcggca
     1321 gccgcaccag ccgctcgctg tagtgcgcgt ccgcgtctcc gggctcgaac gcctcggccg
     1381 agagatacgc gtcgatcgtc ggcagcccgg tggtctcggg atgcccccac gccgcggcct
     1441 gcacgggcgc gagccgcagc gacgcgagcc gcatttcctg cgagtcctgc gccggcgccg
     1501 ggtagatcag cacctcgggg cgccgccgca ggatcgcgtc gacccactcg cgcgggctgc
     1561 gcatcgggcc gtgctcgaaa tgcgcggcga gcgccgccgc gcgcccggtc tcgtcgtcgt
     1621 gcgtgcggcc gagatggaac accgagagct cgatgcgctc gcggtcgagg cgctcgaccc
     1681 agccctgcgc gaacgcggcc caggtcgagt gccgctgcac ctgcgccgag acgatgccga
     1741 cgcgcagccg tcccgagcgc tctcccgcgg ccggcgcacc gatgcgctcg cgctcgagcc
     1801 agccgcgcat cgtgtccgca cacagccgcc cgtactgcgc gagcagctcg cggtggttgc
     1861 cgtcgtggta cgcgaggtag aacggctgca cggaagcgac gacgcgctcc ggatgcgcga
     1921 tcgcgcgcga agcgacccag tcggagagcg cgcgcagctc ggtcgcggcg gccgacggac
     1981 cggcgtccga cgcggacgcg agcgtgcaca tgcgcgacac gccgagcgcc cagcgcgcct
     2041 gcgcgtcctc gggatcgcgc tcgagcgcgc ggcggaacgc ctgctcgccc tgctcgacgc
     2101 ggccctgcac gtgcagcagg ttgaagatgt tgaccacgtt gtagtgcgcg tcgaccatgc
     2161 ccgggtcgag cgcgagcgcc gcgcggtagg cgcgctcggc ctcgcgcggc tcgcccatca
     2221 tctgcaacac ctggcccagg ttgtagtgcg cgttcggata cgcgggctgc agcgcgaccg
     2281 cccgcttcag gctcgccgcg gcgccgcgca ggtcgttcag ctcggcgagg accgcgccga
     2341 ggttgccgtg gacctcggcg tcatcggcct tgatcgacag ggcgcgcgcg aagcacttct
     2401 tcgcgtccgc cggccggccg gccgcgaaat acgccttgcc gagatcgttc agcacgggcg
     2461 cgctcttccc cgcggcctgc gccgcgcgct cgagccgcgc gattgcgctc tccgtgcgtc
     2521 cctgctgcag ctcgagcagc ccgagcagcc gcagcgcgtc ggcgttgcgc ggctcggcgt
     2581 cgacgacctg gcggtacagg cgcgcggcgg cgtcgaggcg gccggcgcgg tgctcgagca
     2641 ccgcctgctc gagcaggccc gccgactcgc tcacgccctg agcaccgcgg cggcgagcag
     2701 cgcccaggcc gcgaggaagg cgacgccgcc gaccggcgtg accgccccga gcgcgcgcac
     2761 gcccgtcagc gcgagcagat acaggctgcc ggagaagagg acgatgcccg cgagcatggc
     2821 ccagcccgac cacttgagca ggaccgagcc cggcaggtga gcggcgacga gcccgaccgc
     2881 gaccaggccg agcgcgtgcc agaagtggta ctcgaccgcc gtgcgccaga gcgccagcgc
     2941 gtcggcgtcc aggcgcgcct tcagcgcgtg cgcgccgaag gcgccgagca gcaccgcggc
     3001 gagcgccgcg agcgagccga ggacgaggaa gatcttagcc gtggccgcca tgttttacgc
     3061 cgggtaatat acgcgacctt gcgccacgat cccatcgcga catgcccgcc gtcgtcatcc
     3121 ccagcatcga agccttgaag cgcctcgtcg gcaccgaggt cgccgtcagc gactggttcg
     3181 cgctgacgca ggaccggatc cgcgagttcg ccgacgcgac cggcgaccat cagtggatcc
     3241 acgtcgacga ggcgcgggcg gcgaaggagt cgccctaccg gggcaccgtc gcgcacggct
     3301 tcctcacgct ctcgctgctg ccgcacctgc tcggccaggc gatcgacctg cgcggcgcgc
     3361 gcatgggcgt caactacggg ctgaaccggg cgcggttcac cggtcccgtg cccgcggggt
     3421 cgcgcgtgcg cgcgcgcttc gcgcttgccg cttgcgagga catcccgaat ggggtgcaga
     3481 cgacctggac cgtcacggtc gagcgcgagg gcgacgccaa gccggtgctg gtcgcggagt
     3541 ggctcacgcg gcggtacgct tagcggcgat caggctttcg gcgtgaactt ggagagatcg
     3601 tagagcccga gctggcgttc gagcgcgctc accttctcga ccatcatctc gagcccgccg
     3661 gcgggcgcgc gcctctccgc gctcttcatc gcctcgccga gcgcccggta ttcggccgcg
     3721 ccgaccatgc ggcggaacgc cgggaacagc accgtgtcct cgcgcgactc gtgcggacgc
     3781 agcatgcgca cggcgtcgcg cagcgacgtc tgcagctggt tgcgcgcgac cggatcgtcg
     3841 agcgtcgtcc cggcgagctt caggatgcgg tcggtgaggc gccgcccggc ctcgtgctgc
     3901 ttgcgcagga cgcggacgag gtcgacgtgc tcctgcgcgg cggcgcgctc gaagcgcggg
     3961 aagacgtgct tctcctcgag cttctggtgg tggtcctcga cgtagccgcg gacgatctgc
     4021 gccgcgtcgc gcacgacctc gggctgcacc ggctcgtcgg caccggcgag gcggcgcgcc
     4081 gactcctcgt agacgaggag cacccgctcg agcaggccct gcgcatggat caggtcctcg
     4141 accggcgtgg gcgcgggccc ggccgggtcg aggggctcgg cggcgcgcga cggcgcaccg
     4201 agcgcgacca gcgccgcgcc ggacgcgccg gcgaggcgca ggagcttgcg tcgttgcggg
     4261 caccggtcca cggcccgtca gtatcgcgcg ccgaggctcc ggaatatcgc atcccgcgga
     4321 tgacccgttc ggcgcaagcc cgccccggac gaccctccag cgacgtgtgt gttatatttt
     4381 ttttcgggag ccccctggcg gtcagagcac gagaagaacg aggggtcctg gtcaaactcc
     4441 ggaggaggaa atcatgcgaa ccacgaagca aggcgcagtg accgcattcg cggccggcct
     4501 ggcggcggca ttgctggccg cgcccctgac ggcggcggcg caggccaagc tctcggtcgg
     4561 gatgccgacc aaccccccga acatcatcca cacgccggtg ttcatcgccg acgatctcgg
     4621 ctactacaag gccgacggcc tgaaggtcga gttcctctcg ttcgagggcg gcgtgtactc
     4681 gttccgcgcc atgatctcgg gcgacgtcga catcgccgcg gcctcgggct cgttcgcgct
     4741 ggtcgcgcgc tccaacggcg cgccgagcaa gctcatcttc tcgagcgcgc ccaagctcga
     4801 gtcggtgatg atggcgcagg gcgacatcaa gtcggtgaaa gagctgaagg gccgcaagat
     4861 cggcatccag gagccgggcg gcttcgcctt cgtgctcagc atgacggtgc tcggctcggc
     4921 cggcctcaag aaggacgacg tcgagatggt gagcatcctc agcgaggacg tgccgccgct
     4981 cgtcgccggc cagatcgaca ccgcgatcct tcacgtcgac caggagatcg tggcgaagga
     5041 gaagaagccc tcgctgcacc cgctcgcacg cctgtccgag gtcgcgccca ggcagctgta
     5101 cctcgcgtac gcggtcaagg aggacacgat caagaagaag cgcaaggcgc tcgtcgcctt
     5161 caccaaggac aacatccgcg ccgtgcgcat gatctacagc gaccgcaaga aggtcgagcc
     5221 gatcatccgc gagcacaccg gcctcgacgc cccggtcgtc tcgaaggcgc tcgacgtgct
     5281 gatcaagaac tgcgcctggg acgcgaacag cggcttgccg cgcgacatga tcgactacac
     5341 cgtggagcgc atggtcaagg tcggcaacat cgacaagggc aagggcacga cttacgacca
     5401 ggcggtcgac ctctcgatcg cgaacgaggc gttgaaggag ctcggtccct ggaaggggcc
     5461 gacatgcgcc cactgagatg cgcgcgctga acacgagccg gccatgagca agcccgccgc
     5521 gcccgccgtc ccggcggcat cggcgccgcg cgagcgggcg cccggcgtcc ctttgcgcag
     5581 ccggcccgcg gtctaccgca tcgcctcggt gatcgtcggc ctcgcggtgt gggagatcgt
     5641 cggcccgcac atcgacccga tcttcttcac ctacccttcg gcgatcgcgc gcgcggcctg
     5701 ggagatgacg gcgagcggcg agctgccgaa gtacgcctgg gagagcctgc agatcctgtt
     5761 ctacgggatg ctgctcgcga tcggcgtcgg gatcccggtc ggcgtgatca tggcgcgctt
     5821 ccgaaaggtc gactgggtgc tcgacacctg gctgaacgcg ctgtactcga cgccgacggt
     5881 cgcgctcgtg ccgctgctcg tgctgtggtt cggcatccgc gccgaggcca aggtcgtggt
     5941 cgtgttcctg ttcgcgatct tcccggtgct gatcaacacc taccagggcg tgcgcaaggt
     6001 cgacgcgagc ttcctcgagg tcgcgcgctc gttccgctcg agcgagtggc agatgtggcg
     6061 cgacgtcttg ctgccctggg cggtgcccta catcacggcg gggatccgcc tcgccatcgg
     6121 ccgcgggctg gtcggcatga tcatcgccga gttctacacg gcgatgaccg gcctcggcta
     6181 catgatcgtt cgctacgcgc acatcttcaa gatggaccgg accttcgtgc ccgtgatcct
     6241 gctcatgttc ctcggcatcg cgcttaccgc cgcgctcaag tgggtcgagc ggcgaatcgc
     6301 gccctggaac cggacggagg actagcgtgg ccgatcgcat cgcgctcaag cgcgtgggca
     6361 agcgctacgg cgctctgcag gtgtgccgca acatcgacct taccgtcggc gagcgcgaga
     6421 tcgtgaccat cgtcgggccc tcgggctgcg gcaagaccac gctgctccgc tgcatcgacg
     6481 gcctgatccc ctacgaggac ggcgacatcc tggtcgaggg cgagcgcatc accgagccgc
     6541 gcgcgggcgt ggccatggtg ttccagcact tcggcctctt cccctggaag acggtgtacg
     6601 acaacgtcgc ctacgggctg cgcatccgcg gcgagccggc cgagcgcatc gccgccgcgg
     6661 tgcccgagta cgtcaacatg gtggggctga gcggcttcga gcggcactat ccctaccagc
     6721 tctcgggcgg gatgcagcag cgctgcggcc tcgcgcgcgc gctcgcggtc gagccgaaag
     6781 tgctgctgat ggacgagccg ttcgcggcgg tggacgcgca gacgcgcgag atcctgcagt
     6841 tcgagctgct gcggatctgg gaacagcggc cgaccaccat gatcttcgtc acgcactcga
     6901 tcgaggaggc ggtgctgatg ggcgaccgcg tcgtcatcct caaggggcgc cccagcggcg
     6961 tgcacgaggt ggtcgacgtc ggcatcccgc atccgcgcag ccgcgcgacg ctcgccgagc
     7021 ggcgcttcgc cgaggtgcgc gagcacgtct ggagcgcgct catggacgag gcgcgcaagg
     7081 cggagtacga gctcgggcgc tagcccgatg ggggaggcgg cctttgcgct cgcggtcgcc
     7141 gcgctcgcct ggctctggta cgacagcatg cgcgcgcgcg agcgcgcggt cgccgcggga
     7201 cgcgtcgcct gcgagcgcga cgggctgcag ctgctcgacg acacggtgga atgcgtctcg
     7261 gtgcggcccg cgcgtgcgct ttccgggcac gcgcgcctgc gccggatcta ccgcttcgag
     7321 ttctccgaca ccggcgacaa ccgccgcgcg ggacggatcg tcatgctggg cgccgaggta
     7381 gaatcgctga cgatggatcc ttttctcctg cggcacccgc aatgaaaccg tcatcgaacg
     7441 gcggctgaca tgtcgcgctg ggtcgaaggc acggtcgcgg ggcagaagcg ctggaccgag
     7501 cgactgtact ccctgtcggt gaacgccgac gtcaagtcgt tcgaggcggg acagttcacc
     7561 aagctcgcgc tcgcggtcgg cggcgagatg gtggcgcggc cgtactcctt cgtgaacggg
     7621 ccgaaggagc ggccgcacga gttctactac atcgtgcttc ccgaggggcc gctcacgcag
     7681 cggctctcgc agctcgagac gggcgacaac gtctatctcg cgcctcagcc gtcgggcttc
     7741 ctcgtgctct ccgaggtgcc cgacgcggag atcctgtggc tgatctcgag cggcaccggc
     7801 gtcggcccgt tcctgtcgat cctcgcgacc gaggcgccct ggcagcgcta tcgccaggtg
     7861 gtgctggtcc aggccgtgcg ccagccggag gaactaacct accgggaccg catcgaaagc
     7921 ttgtcctccg cgcacgtcgg gcagatgcgc cacgttccct tcatcagccg cgagcaccgc
     7981 cccccggcgc tgcacgggcg cgtcccgtcg gcgatcgacg acggccgcct cgaagccgcg
     8041 gccggcgtgc cgctctcggc gcagacctcg caggtcatga tctgcggcaa tcccgagatg
     8101 atcaccgaca ccacggaggc gctcaaacgc cgcggcatgc gcaagcaccg ccgccgcgag
     8161 cccgggcaca tcaccgtgga gaactactgg tgagaatccc ggcctcgatc gcgatcgccg
     8221 ccgcgctgct gctggccggg tgcgcgggca tgacgcgcat cgcgtacaac aacgccgact
     8281 tcttcctgcg caagatggcc aacgactact tcgcgctcga cgatgcgcag gccgaccagc
     8341 tgcgggtgcg cctgcggcgc ttccacgcct ggcaccgcgc gaacgagctc ccgcagtacg
     8401 tcgccttcct gcgcaccgcg cagcgccgct tcacgaaggg actcacgccc gaggacgtcg
     8461 cctgggggat gtcgaccgtg cgcgcccgct tccgcgcgtt cgcggcgaag gcggccgagg
     8521 aggcggcgcc gatgctctcg agcctggcgc cggagcagat cggcgtgctc gagcgcaggc
     8581 tcgccgagaa caacgccaag tacgtgcgcg agaacctgcc ccccgacgag cgcaagcgga
     8641 tccgcgtgca gacgcggcgg atggccgacg cgctgagtca gtgggcgggc gacctgacgc
     8701 gcgaccagga ggcgcgcgtc gagcgcttcg tgcgcgcgca cgaccgcttc gccatgatgc
     8761 gcttcgagga ccgcgtgcgc ggccagcgcg aggcggtcgc gctcctgcgc aagcgcctgc
     8821 cgccgcagga gctcgcgccg ctcctcgcgc ggcacttcaa cgaccccgac gttcggcgct
     8881 ccgacgagtt ccgccgcgag gagaagcgct gggaggccga cttcggcagg ctgctcgcgg
     8941 agctgtcggg gacgctctcg cccgagcagc gcgcccacgt gatcgacaac atcgagcgct
     9001 tcgccgacga cttcgcgatc ctcgccggcg acgcgcgcaa cgcggcatga aggtgcgcgc
     9061 ggtcgcgctg gtggtgctgc tcttgcttat tgccggcgcg cgggccgccg agctcaagcc
     9121 ctggacgggc gacgcgacgc cgcccgccct cgcgctcaag gacctcgagg gccgcgagca
     9181 ccggctcgcc gactaccgcg gcaaggtggt gctcgtgaac ttctgggcga cgtggtgcga
     9241 gccctgccgg gaggagatgc cggccatgca gcgtctccag cagcgcctcg agggcaagcc
     9301 cttcgtcgtc ctggccgtcg acttcggcga aggcgacgcg cgcgtgaagg gattcctgaa
     9361 ggagcttccg ctgccgttca cgttcctgct cgaccgcgac agctccgtcg cgcgcgcctg
     9421 gcgcgtgcgc gtgctgccgg tcagcttcct gatcgacgcc gaccagcgga tccgctactc
     9481 cgcggtcggc gatgccgcct gggactcgcc ggccgtcgag caggtcgtgc gccgtctgct
     9541 gccccgtgcc gccttgcgcc gggcagggca taatgccggc atcgcccgtt ccggcgatca
     9601 ccccagtccg tagaaggagg ctccgttcat ggatcgacgt tcctttctga agtccggcgt
     9661 gctgctcccc gccgccgccg ccccccgatt cgcgaccgcg cagcagctgc ccttccagcc
     9721 ccggcaggcc cagagctggc gcatgttcga ggtcaccacg cgggtcgacg tgctcgtccc
     9781 cggcggcacg accaaggtct ggctgccggt gccctcggtc gactcgacct accagaaggt
     9841 gatcgacaac acttggtcgg gcaacgccag caccgcgaag ctcgtccacg acggcaagta
     9901 cggtgccggc atgctgtacg cggagtggcc ggatgccgag aacaagccct acgtcgagct
     9961 gcacagccgc ttcatgacgc gcgaccgcgc ggtcgacttc ggctcgcgcc tgacgcgcgt
    10021 cggcgaccgg ctgacgcccg aggagcgccg cttctacacc gccccgaccg accatatccc
    10081 ggtcgacggc atcgtgctgc agaccgcgcg cgacgtcacc aagggcacga agaccgacca
    10141 cgacaaggcg aaagcgatct acgagtggat cgtcgacaac accttccgcg acccgaaggt
    10201 gcgcggctgc ggcaccggcg acatcaaggc gatgctcgag accgggaacc tgggcggcaa
    10261 gtgcgccgac ctgaacgcgc tctacgtcgg gctcgcgcgc gcgtccgggc tgccggcgcg
    10321 cgacgtctac ggcgtgcgcg tcgccaagtc gcaattcggc taccgcagcc tcggggcggg
    10381 aacggcgaac atcacgcgcg cgcagcactg ccgggccgag gtctacctcg aggcctgggg
    10441 ctgggtgccg gtcgatccgg ccgacgtgcg caaggtcgtg ctcgaggaga aggcgcagcc
    10501 gaccacggtc gacgatccgc tggtgcaggc ggtgcggccg aagctcttcg gcgcctggga
    10561 gatgaactgg ctcgcgttca acacagcgaa cgacatcgcg ctgccgaact cgcagtacgc
    10621 gaagctcacg ttcctcatgt acccgcaggc ggagaccgcg gccgggaggc tcgacagcct
    10681 cgaccccgac aacttccgct attcgatgag ctcgaaggag ctgaccgcct gacatccagg
    10741 ccggtcctgc acttctcgca cgcgaacggc tttcccgcgc cgagttaccg caagttcttc
    10801 gcggggctcg agcgcgactt cgagatcggc tggatcgagc gcatcggcca cgacccgcgc
    10861 tatcccgtca ccgatggctg gccgcatctc gtcgacgagc tgatcgggca cgtcgaatcg
    10921 acctatcgcg atccggtggt cgccgccggc cactcgctcg gcggcttcct cacgctgcgc
    10981 gccgccgcgc gccgcccgga cctgttccgc gcgatcgtcg tcatggactc gccgatcccg
    11041 aagcgctggt cgggcaccgc gctgcagatg gtgaagcgcc tcgggctggt cgaccgcgtc
    11101 actccggccg ggaccacccg cggtcggcgc tcggaatgga gctcggcgga ggaggcggtc
    11161 gagcacttcc gcggcaagcg caatttcctg cgcttcgacc ccgagtgcct gcgcgactac
    11221 gtcacgctcg gcatgcgccg cgacgggcgc ggggtgcggc tcgcgttcga tcccgggatc
    11281 gagtaccgga tctaccgcac catcccgcac gatctcgcgc gcgatctccc ggcgctcaag
    11341 gtgcccgccg gcttcgtgtg tggagaggaa tcgagcgaga tgcgaaggcc ggggctggtc
    11401 gatccacgcc ggcatttccg cgtggcacgc gtgcccggcg ggcacctgtt cccgttcgag
    11461 cggccggccg aggcggccgc cgcggtcggc gcgctggcgc tcgacctggc gatgccggcc
    11521 tagaacctca gccgcggccc tcgagcagcg cggcgagcct gccgaaatcg agcggcttgg
    11581 cgaagtggtg gtcgaagccc tcggcgatcg cgtgcgcggc gtcgcgctcc tgcgcgaacc
    11641 cggtgagcgc gatgatggtc atcgacgccg ccgcggggaa cgcgcgcagc cgccgcagta
    11701 cttcgtatcc gtccatcgtg ggcatgccga tgtcgaggat cacgatctcg ggccgcaggc
    11761 acgccgcctg gcgaagggct tcggcggcat cgctcgtgca cgtc
//
LOCUS       DATDMW010000019         4754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_999184, whole genome shotgun sequence.
ACCESSION   DATDMW010000019 DATDMW010000000
VERSION     DATDMW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999184"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VI319_00595"
     CDS             complement(<1..1016)
                     /locus_tag="VI319_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6862372.1"
                     /translation="MTRKVRYAVVGLGHIAQVAVLPAFAHARENSELAALVSGDPVKR
                     ETLARRYGVPRACGYDEYEDCLRAGDIDAVYIALPNSMHREYAERAADAGVHVLCEKP
                     MAVTEDECEAMIRAARRNGVRLMIAYRLHFEQANLEAIEIVRSGRIGEPRLFNATFTM
                     HVADGNIRLERRMGGGTLYDIGIYCINAARNLFRDEPIEVQAACAGRRGDVEEAASCV
                     LRFPGERLAAFTCSFGAGKVSTCQVVGTRGDLRLDPAFEYAGTLRHRLSVGEHVEERA
                     FDKRDQFAPELVHFSECVLAGREPEPSGAEGLADVRLIRALYESARTGRPVKPEPVER
                     VRRP"
     gene            1305..2114
                     /locus_tag="VI319_00600"
     CDS             1305..2114
                     /locus_tag="VI319_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY6862373.1"
                     /translation="MTSGGFTVGRVAGIEVYVHASLLIVLALVSFSLATGLLPAWHPA
                     WSASMRWFVALVASMLLVASVAIHELAHAWVGRAGGIPVWRVTLFVFGGMAHLEAEPR
                     SWRVELWTALAGPGASLALGASFVALGVALGARESVDAQPGHLLAAIGPMATLLLWLG
                     QINIVLAAFNMLPGFPLDGGRVLRALIWRSSGDMLGATRFAARSGQALSCLLVGVGFA
                     MVFGLRLPLLGGGPAAGTWIALMGWFLNAAALAGYRDVTLRAEVRPLAKAS"
     gene            complement(2151..3542)
                     /locus_tag="VI319_00605"
     CDS             complement(2151..3542)
                     /locus_tag="VI319_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6862374.1"
                     /translation="MPHALVVDDDTNALSGLAELVAREGFSVAAASTLREARARMNEQ
                     RPEVVFLDLQLPDGNGMDLFEDVKSRAATEVVLITGHASIESSIEALRLGAADYLIKP
                     VNIKQVKAILSRVARPADLKSEIGALRDELRRLGRFGQLLGTSAPMQRVYDQIERVAP
                     TAATVLITGESGTGKELVAQTLHNLSRRRKQPFLPINCGAISPQLIESEMFGHEKGSF
                     TGAMREHKGYFERASGGTVLLDEITEMPVELQVKLLRVLESGTFMRVGSDREIEVDVR
                     VLAATNRNPDEAVAEGKLREDLLYRLRVFPLHLPPLRERGEDVVLIASHFLDDINGVE
                     GTAKTFAPATLDRLRGYDWPGTVRELKNVVHRAFIMADDLIDAHCLPPELGHAKQVTG
                     PYFQVRVGSKVTDVERRLILATLEQCGGTKEKAAEMLGISLKTLYNRLREYQAQKDPA
                     ADTVYEEPGTRVG"
     gene            3933..>4754
                     /locus_tag="VI319_00610"
     CDS             3933..>4754
                     /locus_tag="VI319_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY6862375.1"
                     /translation="MTDYDAYLFKQGTHAHLHERLGAHFTADGAAVRFAVWAPNAAAV
                     SVIGDWNGWNPENLQLRPRDDGSGVWEAVAQGVPHGACYKYRITSHNMGYQVQKADPF
                     AVCTEEPPRTASRVWRLDYDWGDGDWMRSRRRANALDAPISVYEVHLGSWRRSPDTPH
                     RVLGYRDLARALAAYVKEMGFTHVELMPVTEHPFYGSWGYQTTGYFAPSARYGTPQDF
                     MWFVDHLHQNGIGVILDWVPSHFPSDEHGLAYFDGTHLYEHADARQGFHPDWTSYI"
BASE COUNT          669 a         1679 c         1646 g          760 t
ORIGIN      
        1 tccggccggc gcacgcgctc gacgggctcg ggcttcaccg gcctgccggt ccgcgccgac
       61 tcgtacagcg cgcggatcag tcgcacgtcg gcgagcccct ccgctcccga gggctcgggc
      121 tcgcggcccg cgagcacgca ctcggagaag tgcacgagct cgggcgcgaa ctggtcgcgc
      181 ttgtcgaacg cgcgctcctc gacgtgctcg ccgacgctca ggcgatggcg cagtgtgccc
      241 gcgtactcga acgccgggtc caggcgcaga tcgccgcgcg tgccgacgac ctggcaggtc
      301 gagaccttgc cggcgccgaa gctgcacgtg aacgcggcga gccgctcgcc ggggaagcgc
      361 aggacgcagc tcgccgcctc ctcgacatcg ccgcggcgcc cggcgcacgc cgcctgcacc
      421 tcgatcggct cgtcgcgaaa aagatttctc gcggcgttga tgcagtagat gccgatgtcg
      481 tagagcgtgc cgccgcccat gcgccgctcc aggcggatgt tgccgtcggc gacgtgcatg
      541 gtgaacgtgg cgttgaacag ccgcggctcg ccgatgcgcc ccgagcgcac gatctcgatc
      601 gcctcgaggt tcgcctgctc gaaatgcagc cggtaggcga tcatgagccg cacgccgttg
      661 cgccgcgcgg cgcggatcat ggcctcgcac tcgtcctcgg tgacggccat cggcttctcg
      721 cacagcacgt gcacgcccgc gtccgcggcg cgctcggcgt actcgcggtg catgctgttc
      781 ggcagcgcga tgtagacggc gtcgatgtcg ccggcgcgca ggcagtcctc gtactcgtcg
      841 tagccgcacg cgcgcgggac gccgtagcgc cgcgcgagcg tctcgcgctt caccggatcc
      901 cccgagacga gcgccgccag ctcggagttc tcgcgcgcgt gcgcgaacgc cgggagcact
      961 gcgacctgcg cgatatggcc gaggccgacg accgcgtagc ggacttttcg ggtcatggca
     1021 tcccggcgat cagcaataag cacgccaaac cgccgccgcg gcgctgcgtc ggggcaccgc
     1081 gacgctggcg cggcttcgca gaaattctta cacgcgcacg gaacaattta ccggcccggg
     1141 cggcctatgc cggcgcccgg tgcggcacgc aatggccgac ctccgtggca tggaaactgc
     1201 ctttccaagg tcggaacatt gacgatgcga agaatcgcgc ctttcgccgc gctcgccgcg
     1261 acgccgccgg gctcggccta cgcgggaatc ctggtgtcgc cgccatgacc agcggaggat
     1321 tcaccgtcgg acgcgtcgcg ggcatcgagg tctacgtcca cgcgagcctg ctgatcgtgc
     1381 tcgcgctggt gagcttcagc ctcgcgaccg ggctgctgcc cgcgtggcat cccgcctgga
     1441 gcgcgtcgat gcgatggttc gtcgcgctgg tcgcgtcgat gctgctggtg gcgtcggtcg
     1501 cgatccacga gctcgcgcat gcctgggtcg gccgcgccgg cggcatcccg gtgtggcgcg
     1561 tcacgctgtt cgtgttcggc gggatggcgc atctggaggc ggagccgcgc tcgtggcgcg
     1621 tcgagctgtg gacggcgctc gcgggcccgg gggcgagcct ggcgctcggc gcctcgttcg
     1681 tcgcgctcgg ggtggcgttg ggcgcgcgcg agtccgtcga tgcgcagccc ggacacctgc
     1741 tcgccgccat cggaccgatg gcgacgctgc tgctctggct cggtcagatc aatatcgtgc
     1801 tcgcggcatt caacatgctg cccgggttcc cgctcgacgg cggacgcgtg ctgcgcgccc
     1861 tgatctggcg ctcgagcggc gacatgctcg gcgcgacgcg cttcgccgct cgcagcgggc
     1921 aggcgctctc gtgcctgctg gtcggcgtcg gcttcgcgat ggtgttcggc ctgcgcctgc
     1981 cgctgctcgg cggcgggccc gcggccggaa cctggatcgc gctgatgggc tggttcctga
     2041 acgccgccgc gctcgcgggc taccgcgacg tgacgctccg cgccgaggtg cgccccctcg
     2101 cgaaggcgtc gtgacgccga tggccgggta aggtcacccg cccggcggga tcagccgacg
     2161 cgcgtcccgg gctcttcgta tacggtgtcg gccgccggat ccttctgcgc ctggtactcg
     2221 cgcagccggt tgtacagcgt cttcaggctg atgccgagca tctcggcggc cttctccttg
     2281 gtgccgccgc attgctcgag cgtcgcgagg atgagccggc gctcgacgtc ggtgaccttg
     2341 ctgccgacgc gcacctggaa gtacggcccc gtcacctgct tggcatgccc gagctcgggc
     2401 ggcaggcagt gcgcgtcgat caggtcgtcg gccatgatga aggcgcggtg gacgacgttc
     2461 ttcagctcgc gcacggtgcc gggccagtcg tagccgcgca gccgatcgag cgtcgccgga
     2521 gcgaaggtct tcgccgtccc ttcgacgccg ttgatgtcgt cgaggaaatg gctggcgatc
     2581 aggaccacgt cctcgccgcg ctcgcgcaag gggggaaggt gcagcgggaa cacgcgcagg
     2641 cggtacagca ggtcctcgcg cagcttgcct tcggcgacgg cctcgtcggg gttgcggttg
     2701 gtggcggcga gcacgcgcac gtcgacctcg atctcgcggt cggaaccgac gcgcatgaag
     2761 gttccggact cgagcacgcg cagcagcttc acctgcagct cgaccggcat ctcggtgatc
     2821 tcgtcgagca gcacggtgcc gccgctcgcg cgctcgaagt agcccttgtg ctcgcgcatc
     2881 gcgccggtga agctgccctt ctcgtgcccg aacatctcgc tctcgatcag ctgcggcgag
     2941 atcgcgccac agttgatcgg caggaacggc tgcttgcgcc ggcgcgacag attgtgcagc
     3001 gtctgcgcaa cgagctcctt gccggtgccg ctctcgccgg tgatcagcac cgtcgccgcg
     3061 gtcggcgcga cgcgctcgat ctggtcgtag acgcgctgca tcggcgccga cgttccgagc
     3121 agctggccga agcggcccag gcggcgaagc tcgtcgcgca gcgcgccgat ctccgacttc
     3181 aggtcggcgg gccgcgcgac gcgcgacagg atcgccttga cctgcttgat gttgaccggc
     3241 ttgatcaggt agtcggcggc gcccagccgc agcgcctcga tcgagctctc gatgctcgcg
     3301 tgcccggtga tgagcacgac ctcggtcgcg gcgcgcgatt tcacgtcctc gaacaggtcc
     3361 atgccgttgc cgtcgggcag ctgcaggtcg aggaagacga cctccggccg ctgctcgttc
     3421 atgcgcgcgc gcgcctcgcg cagcgtcgac gcggccgcta cgctgaagcc ctcgcgcgcg
     3481 accagctcgg cgagcccgga aagggcgttg gtatcgtcat cgactacgag agcgtgggga
     3541 atgggaggct tccttcaaag agcgctgcga aaaccgcgcg cgagcgacga gcgtcgctgc
     3601 agattaattt agcacgattc cactcggtga cacggcggga gggaccgatg gaaccattca
     3661 cggatttctt tgccttatct cggtcgaagc gacatgacga agccaacacc gggagagtcg
     3721 cgcgatcgtc cccatactcg agttccaggc gcccgcgtcc tccggacgga cgggtcggcg
     3781 ccgtcgaccg aacgacataa acccctagaa aacagggcat tgccttccct cctccgccat
     3841 catgatctgt tctgaaaggc accgacgtgg cgaccacgca tgacacccgg gcgtcccgcc
     3901 gcgaggctcc ggcgtcccgg acccgcgatt ctctgaccga ctacgacgcc tatttgttca
     3961 aacaaggcac gcacgcgcac ctgcacgagc gcctcggcgc gcacttcacg gccgacggcg
     4021 ccgcggtgcg cttcgcggtg tgggcgccga acgccgccgc ggtctcggtg atcggcgact
     4081 ggaacggctg gaatcccgag aacctgcagc tgcgcccgcg cgacgacggc tcgggcgtgt
     4141 gggaggcggt ggcgcagggc gtgccccacg gggcctgcta caagtaccgg atcacctccc
     4201 acaacatggg ctaccaggtg cagaaggccg atcccttcgc tgtctgcacc gaggagccgc
     4261 cgcgcaccgc gtcgcgcgtc tggcgcctcg actacgactg gggcgacggc gactggatgc
     4321 gctcgcgccg gcgcgcgaac gcgctcgacg cgccgatctc ggtgtacgag gtgcacctcg
     4381 gctcgtggcg gcgcagcccg gacacgccgc accgcgtcct cggctaccgc gatctcgcgc
     4441 gcgcgctcgc cgcctacgtg aaggagatgg gcttcacgca cgtcgagctg atgccggtga
     4501 ccgagcatcc cttctacggc tcgtggggct accagacgac gggctacttc gcgccgagcg
     4561 cgcgctacgg cacgccgcag gatttcatgt ggttcgtcga ccatctgcac cagaacggca
     4621 tcggcgtgat cctcgactgg gtgccgtcgc acttcccgag cgacgagcac ggcctcgcct
     4681 acttcgacgg cacacatctt tacgagcacg cggacgcccg ccagggtttt caccccgact
     4741 ggacgagcta catc
//
LOCUS       DATDMW010000020         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1248199, whole genome shotgun sequence.
ACCESSION   DATDMW010000020 DATDMW010000000
VERSION     DATDMW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248199"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VI319_00615"
     CDS             <1..183
                     /locus_tag="VI319_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY6862376.1"
                     /translation="VRGAIEAGHLRPGTDPWQVTFEIYGMILAAHHERRLLGDPRAAF
                     RAQAAFERLVGAHAPA"
     gene            253..324
                     /locus_tag="VI319_00620"
     tRNA            253..324
                     /locus_tag="VI319_00620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:283..285,aa:Gly,seq:tcc)
     gene            334..642
                     /locus_tag="VI319_00625"
     CDS             334..642
                     /locus_tag="VI319_00625"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY6862377.1"
                     /translation="MQLGIIIAIAIAIAAVAFALQNNVPVTVTFLVWRFDSSLAMVLL
                     LALAIGAIVMALVTAPNALRARWTLSRQRKEISDLRSQVSRPGPEPSEAAAPKIETPL
                     "
     gene            700..1173
                     /locus_tag="VI319_00630"
     CDS             700..1173
                     /locus_tag="VI319_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY6862378.1"
                     /translation="MGTRAALGLCTALAALALAGCGGAPTRPDAAPAASRPAAEQAAG
                     FALKMVGRPYRFGGSTPAGFDCSGLVQYSYRQAGVRLPRSTEAQLVTGTRVRLSSVRR
                     GDLLFFDEEGKKKSHVGIYLGEGRFVHAPSSGKHVRTDSLDAPYWKKHFAEARRV"
     gene            complement(1175..1855)
                     /locus_tag="VI319_00635"
     CDS             complement(1175..1855)
                     /locus_tag="VI319_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY6862379.1"
                     /translation="MARERSSRAALRARWLKIDTATVADVLDTMGAPDQGLAASFRPF
                     PATAGKLAGWAYTLQGSMQRYAGSGDPDKMKAVDGVSAGEVTVWAGDGEGVCFFGELI
                     ALGMKRRGCAGALIDGGIRDIEWIAKQKFPVFARYRTPVQSIGRWKVAAWQVPVELPG
                     ATVKRVAVRPGDFVLADVDGAIVIPAGIADAVLAQAEKLTATERKIRRDLDRGATLED
                     VLKKYGHV"
     gene            complement(1845..>2585)
                     /locus_tag="VI319_00640"
     CDS             complement(1845..>2585)
                     /locus_tag="VI319_00640"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEY6862380.1"
                     /translation="DATVVAVAGRSEASKGKTLAAKHRVPFVVGLDALFAAKPDVVIE
                     AASHQAVRDCGEAVLSKGISFIVLSGGALCDDALRARLEAAAQKAGAMLYVPSGGIGG
                     MDALKAACVAGVDHVEIAVTKPPAAWKGIAYVEKLGVDLDRLAGATVLFEGKARDGVP
                     LFPQNVNIAALLSMAGIGFDRTQLKVVADPALKFNTHTITIRGKSGTISLKFESVPFP
                     DNPKTAMLAAYSALAAFKQFNSRVRYGT"
BASE COUNT          359 a          970 c          875 g          381 t
ORIGIN      
        1 gtgcgcggcg cgatcgaggc cgggcacctc cgccccggga ccgatccctg gcaggtcacc
       61 ttcgagatct acggcatgat cctcgccgcg caccacgagc ggcgcctgct cggcgacccg
      121 cgcgccgcgt tccgcgccca ggccgcgttc gagcggctgg tcggggcgca cgcgccggcc
      181 tgagcccggc gtccttcgga ctatcccgcg cgtcatcgcc gggaagcgtc ggacggctac
      241 agtcgcgggg acgcggaagt agctcaggga gagcgcgcgg gttccacccg cgagggcaac
      301 gggttcgatt cctgttcttc cgccccggac cttatgcagc tcggcatcat catcgccatc
      361 gcgatcgcga tcgcggcggt cgcgttcgcg ctccagaaca acgtccctgt cacggtgacg
      421 ttcctggtgt ggcgcttcga cagctcgctc gcgatggtgc tgctgctcgc gctcgcgatc
      481 ggcgccatcg tgatggcgct cgtcacggcg ccgaacgcgc tccgggcgcg ctggacgctg
      541 tcgcgccagc gcaaggagat ctcggatctg cgctcgcagg tctcgcggcc gggccccgag
      601 ccgtccgagg cggcggcgcc gaagatcgag acgccgctct agggcggccg tgaaaatccg
      661 cacagcgcga cccgggaccg cgctattatc ctgcccccaa tgggtacccg agccgctcta
      721 ggcctctgca ccgccctcgc ggcgctcgcg ctcgcgggct gcggcggcgc gcccacgcgc
      781 cccgacgcgg cgcccgccgc gtcgcgaccg gcggccgagc aggcggccgg cttcgcgctg
      841 aagatggtcg ggcggccgta ccgcttcggc ggctcgacgc ccgccggctt cgactgctcg
      901 gggctggtgc agtacagcta ccgccaggcc ggcgtgaggc tgccgcgcag caccgaggcg
      961 cagctcgtga cggggacgcg cgtgcggctg tcgagcgtgc ggcgcggcga cctgctgttc
     1021 ttcgacgagg aaggcaagaa gaagtcgcac gtcggcatct acctcggcga gggccgcttc
     1081 gtgcacgcgc cttcgtcggg caagcacgtg cgcaccgaca gcctcgacgc gccttactgg
     1141 aaaaagcact tcgccgaggc ccgccgcgtc tgattcagac gtggccgtat ttcttcagca
     1201 cgtcctccag cgtcgcgccg cggtccaggt cgcggcggat ctttctctcc gtcgcggtca
     1261 gcttctccgc ctgcgcgagc accgcgtccg cgatcccggc cgggatcacg atcgcgccgt
     1321 cgacgtcggc gagcacgaaa tcgcccggcc gcacggcaac gcgcttcacc gtcgcgccgg
     1381 gcagctcgac cggcacctgc cacgcggcaa ccttccagcg gccgatcgac tgcaccggcg
     1441 tgcggtagcg cgcgaacacg ggaaacttct gcttcgcgat ccattcgatg tcgcggatgc
     1501 cgccgtcgat cagcgcgccg gcgcagccgc gccgcttcat cccgagcgcg atcagctcgc
     1561 cgaagaagca cacgccctcg ccgtcgccgg cccacaccgt cacctcgccc gcggagacgc
     1621 cgtcgaccgc cttcatcttg tccggatcgc ccgagcccgc gtagcgctgc atcgagccct
     1681 gaagggtgta ggcccacccg gccagcttcc cggccgttgc cgggaacggg cggaacgacg
     1741 ccgcgagccc ctggtcgggc gcgcccatcg tgtcgaggac gtcggcgacc gtcgccgtat
     1801 cgatcttcag ccagcgggcg cgtagcgccg cgcggctcga gcgctcacgt gccatagcgc
     1861 acgcgcgagt tgaactgctt gaaggccgcg agcgcgctgt aggcggcgag catcgcggtc
     1921 ttcgggttgt cggggaacgg caccgactcg aacttcaggc tgatcgtccc cgacttgccg
     1981 cggatggtga tggtgtgcgt gttgaatttc agcgcggggt ccgcgacgac cttgagctgc
     2041 gtgcggtcga acccgatccc cgccatggaa agaagcgccg cgatgttcac gttctgcgga
     2101 aagagcggca cgccgtcgcg cgccttgcct tcgaacagaa ccgtcgcgcc tgccaaacga
     2161 tcgaggtcga cgcccagctt ctcgacgtag gcgatgccct tccacgccgc cggcggcttg
     2221 gtcaccgcga tctcgacgtg atcgaccccc gcgacgcacg ccgctttgag cgcgtccatg
     2281 ccgccgatgc cgccggaggg gacgtagagc atcgcccccg ctttctgcgc cgccgcctcg
     2341 agccgcgcgc gcagcgcgtc gtcgcacagc gcgccgcccg agagcacgat gaacgagatg
     2401 cccttcgaca gcaccgcctc gccgcagtcg cgcaccgcct ggtgcgacgc ggcctcgatg
     2461 accacgtcgg gcttcgccgc gaacagcgca tcgaggccga cgacgaacgg gacgcggtgc
     2521 ttcgcggcga gcgtcttgcc cttcgacgcc tcgctgcgtc cggccaccgc aaccaccgtc
     2581 gcgtc
//
LOCUS       DATDMW010000021        11472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1497752, whole genome shotgun sequence.
ACCESSION   DATDMW010000021 DATDMW010000000
VERSION     DATDMW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497752"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VI319_00645"
     CDS             complement(<1..982)
                     /locus_tag="VI319_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862381.1"
                     /translation="MITRAFGAGDDHPLHNEAEVKKMIAGLPADPAAALAELVHWAVS
                     TNAHERYSPEHRARSLAAMDAAAHERWPAVCGACLAPGGKPAEGRDLDNATIRSLLEI
                     ATAFANGHKASLAGSASEGGWIRRNIAPLTLRRAVWLARRLTLANMMHQPNIASMWEE
                     AHVLYKQAGVQQVLAAAQQLSADAPLTSVKQEYVRMLMMDIAGQDTMNARDIELAFRI
                     AGRVAASARLELEPILGALYVVVPQGALRPMPVRRLASGPALFLDARPCALALQATLL
                     SDPSRRSARPDVLFGGHFTERETFDMAQRLADYWGPTPPKRRSQRVALNAQ"
     gene            complement(1066..3255)
                     /locus_tag="VI319_00650"
     CDS             complement(1066..3255)
                     /locus_tag="VI319_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY6862382.1"
                     /translation="MNLAMEEAIRIEDRWYVLATHSRHGDPTRVLKHGETFGLFDGYG
                     DIPRVGVGEHGIYHQGTRFLSHYELRINGQKPTLLNSSVKQDNSQLHCDLTTPDLHEG
                     PGENAKLEVLKGTVHLARSRLLWDAACHERLEIANYGAQPVALRLTLEFAADYADIFE
                     VRGYRRERRGQELPPELSPARVRLGYRGLDGVVRRTDLDCSPPPETIAPDHVAYELVL
                     APRATAGIEITVTCDPDARERAPMDFAKALLCTEATLRSVRARSASVETTNQQFNHWL
                     FRSSADLTMLTAGNPEGPYPYAGVPWYSTPFGRDGILTAMEYLWADPSVARGVLAFLA
                     RTQADRVIPERDAEPGKIVHEMRKGELANLGEIPFGCYYGTIDSTPLFVALAGAYYQR
                     TGDLEFVRRLWPHVERALDWMREYGDVDGDGFLEYQRKSAEGLANQGWKDSEDAVFHR
                     DGALADGPIALAEVQGYAYLAKMRAARLAQALGAAERADTLRREAGDLRERFERAFWS
                     EELESYVIALDGRKRPCVVRSSNAGQVLWSGIASPEHAQRTARTLFSRESFSGWGVRT
                     LAEGEARYNPMSYHNGSIWPHDNALIALGLARYGMKDKTIAILTALFEASLFMDLHRL
                     PELWCGFVRRPSEGPTLYPVACSPQAWASASVFYVLQACLGLSFHPEAPRIRFTHPIL
                     PAWMQRVQIANLRVGDAVVDLDFERHEHDVGVSVVGKSGSIDVSVVL"
     gene            complement(3255..4322)
                     /locus_tag="VI319_00655"
     CDS             complement(3255..4322)
                     /locus_tag="VI319_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6862383.1"
                     /translation="MRVAIVAPLYESVPPRLYGGTERVVSYLTEELVAQGHEVTLFAS
                     ADSRTRARLRPMARQGLRLDPGCRDPMAYHLVMLDQVFESVREFDIVHFHLDYLQLPF
                     ARRLAVPSVTTLHSRLDVPEMQMVHRRFGEVPLVSISHAQRLPMASANWVANVHHGLP
                     PQLYPFSARPGSYLAFLGRISPEKRPDRAIRLARAAGIPLKIAAKIEKLDLQYFDEVI
                     RPMLDGPGVEFVGEIGEADKARFLGEALALLFPIDWPEPFGLVMIESMACGTPVLAWR
                     CGSVPEIMVNGVTGFVVDDERSAAAAIGRMPGLDRAACRRAFEERFSARRMAREYLSV
                     YRRLLEPPEPQASRIGEKENG"
     gene            4497..5432
                     /locus_tag="VI319_00660"
     CDS             4497..5432
                     /locus_tag="VI319_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862384.1"
                     /translation="MPRSVTARVAIPDMISPSYFPAIAAVELGYFREEGIDATLELLY
                     PVTKTYEELREGRLDFVGGAAHAPLYAFPGWKGCKLLAALAQNMYWFLVVRKDLSPRR
                     GDLAVVKGLRIGAAPGPVDGLRRMLREAGIDPEREVKIGPVPGWASAGASFGLMAAHA
                     LEEGKLDGFWANGMGAEVAVRRGVGALVLDARRGDGPAGSQHYTFPALVTTQKWIDEK
                     PDAVAGAVRATARAQAALRADPALATKAASMFPEAERALIAELIRRDAPFYDPAIEEP
                     AARALNDFARDIGILRGAPSYEDTVAVRFRGAWKP"
     gene            5507..5926
                     /locus_tag="VI319_00665"
     CDS             5507..5926
                     /locus_tag="VI319_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862385.1"
                     /translation="MLLLEPEGELLAVSLEPEPLLEDEAEGDELDPLDWSLEVEGELE
                     LELGVLLEALELGELELPLSLFIARLPVDEPEDDEPEGLEGDEDAMPEEPEEPDEPDW
                     DRRESLPRSQAESPKVAAIAAATAVRKNRFCSMSTIS"
     gene            6146..7294
                     /locus_tag="VI319_00670"
     CDS             6146..7294
                     /locus_tag="VI319_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY6862386.1"
                     /translation="MKRSSDRFLTTHTGSLPRPEDLIRTMYAKEEGVPVDRPALGARI
                     RSAVEETVRKQAEAGIDLVNDGEMSKPSYATYVKDRLSGFGGTGNTFVYQDLADFPSL
                     EKRVFGDPGRSRRKTPACNAPIGVRDPEAAAADARNLKDAAARVKTEGTFMSAASPGV
                     VSLFFRNDYYKTQEEYLFAIADAMRHEYETVANAGIVLQIDCPDLAMGRHIQYASLPL
                     AEFRKKIRLHIEALNHALANIPPERLRMHLCWGNYEGPHHCDVPLADIIDVVFAARPA
                     AISFEAANPRHAHEWTLFERVKLPEGKVLIPGVLESKTNFIEHPELVAQRIGRYARLV
                     GPENVIAGTDCGYGTWVGQAAVDPQVVWAKLAAMAEGARIASKQFFGR"
     gene            complement(7349..7774)
                     /locus_tag="VI319_00675"
     CDS             complement(7349..7774)
                     /locus_tag="VI319_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862387.1"
                     /translation="MRSKTLLATIIAGALSAPLAALAHDSSASSGASMSGDSQTQAQS
                     TAPGDNGQLAQNPPCDDSASANCPQSTPTPGDDPSLQSGPSDDSRSAQSDSGNPGAND
                     GQLAQNDTDGTLWSSPSDGSSSPYGSTGDPNLRHSSARA"
     gene            complement(7790..8164)
                     /locus_tag="VI319_00680"
     CDS             complement(7790..8164)
                     /locus_tag="VI319_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862388.1"
                     /translation="MRSKTILAAALAGALAAPLAARADDKNNSSYGSQSPSASANIGG
                     ASVQGSLGSSDRSAGAYGQERNDRYAQNEDHDNGRHRGRDKDKDRDHDKDRDHDQDRH
                     ASQSNSYPSQSSNQQYSANGKY"
     gene            complement(8280..8984)
                     /locus_tag="VI319_00685"
     CDS             complement(8280..8984)
                     /locus_tag="VI319_00685"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6862389.1"
                     /translation="MATPHPNTLTLAPGVVSTSADEVERVLQALLIRKQSLVALDAAG
                     RPHSTWRLALLDAGRQYIVLDPAPESASRTLPERPLATFVAEFGGMQIEFASTDPRPS
                     GHAPASVRLGFPRAIVTRQRRAHPRAQIPQGFPAACIIPVGTGAPLQAEIMDISEAGI
                     GLLLHGVSIVPAAGTRITGCRIESGDQGVIAVDLEVRHSRPVEFADGRRAQRWGCQFL
                     APSQKVKQLIARFATE"
     gene            complement(8995..9756)
                     /locus_tag="VI319_00690"
     CDS             complement(8995..9756)
                     /locus_tag="VI319_00690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6862390.1"
                     /translation="MNASKVAVVTGGGTGIGKSAALALLGAGWSVVLAGRRRDKLEQA
                     LAEAGAGASRALAVQTDVGDPASVRALFAKTRERFGGRLDLLFNNAGIGTPGSVMLED
                     LTVEQWKAVVDTNLTGAFLCTQEAIRIMKSQDPRGGRIINNGSISAHVPRPNSAPYTA
                     TKHAITGLTRSTSLDGRKYDIACGQIDIGNARTDLASRMANGVPQANGTIAAEPLMEV
                     DEVGRAVLYMAGLPLDTNVQFMTIMATKMPFIGRG"
     gene            complement(9787..10488)
                     /locus_tag="VI319_00695"
     CDS             complement(9787..10488)
                     /locus_tag="VI319_00695"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEY6862391.1"
                     /translation="MSEALPAEHITRAPQEIARVLELLASRGAPLLSNVARGTLQFVS
                     RLRQIDPGLRHIVLDACDDEEANEALVSRLRAIFCANVGERYFEFAASNPARMEHDGR
                     PLIRADFPDVLATYARRADARVAISPPLHCLADASGDMPFDAQVIDISAGGVGLLYPA
                     DISLEPGTILRGCVVTGLRMAPSIFDLEVRYSQVALLSEGPYTQRSGCRFVDANALGV
                     RQLVAACMTKGPGAA"
     gene            complement(10485..11156)
                     /locus_tag="VI319_00700"
     CDS             complement(10485..11156)
                     /locus_tag="VI319_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862392.1"
                     /translation="MATSGSKSVVLVVLAAAVAAAAGWGAWSAHQRAELRRAASDLVQ
                     EASAGLRDGLRAHPGSGAGLAQLDADAAGLDARLADFERVRTAPGRALAYAAEEYALS
                     ARQILRELAREERGRQRVDEGARLLVQHMNHANRRTPDFLSRALQIKGDLEKRYFDYG
                     VAADALARELDAFPGARAKLAAQVDASALLDEGLAADARRRAAESASRVADEVQRARD
                     IAGPR"
     gene            11256..>11472
                     /locus_tag="VI319_00705"
     CDS             11256..>11472
                     /locus_tag="VI319_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862393.1"
                     /translation="MKAIVIREFGPPEVMRLEDVPTPEPGPGEVVVRVHAVSVNRTLD
                     LILRAGAYALPIELPHVLGVDPAGVVSA"
BASE COUNT         1633 a         3987 c         4161 g         1691 t
ORIGIN      
        1 cctgcgcatt gagcgcgacg cgctgcgagc ggcgcttggg cggcgtcgga ccccagtagt
       61 cggcgagccg ctgcgccatg tcgaacgttt cgcgctcggt gaagtgcccg ccgaacagca
      121 cgtcgggcct ggccgagcgg cggctcgggt cggagagcag cgtcgcctgc agcgcgagcg
      181 cgcacggccg ggcgtcgagg aacagcgcgg gaccgctggc gaggcggcgg accggcatcg
      241 gccgcagcgc gccctggggc acgacgacgt agagcgcccc gaggatgggc tcgagctcca
      301 ggcgcgcgct cgcggcgacg cgtccggcga tgcggaaagc gagctcgatg tcgcgcgcgt
      361 tcatcgtgtc ctgcccggcg atgtccatca tcagcatgcg cacgtattcc tgcttgaccg
      421 acgtgagggg cgcgtcggcg gacagctgct gcgccgcggc cagcacctgc tgcacgccgg
      481 cctgcttgta gagcacgtgc gcctcttccc acatcgaggc gatgttcggc tggtgcatca
      541 tgttggcgag cgtgagcctg cgcgcgagcc aaacggcccg gcgcaaggtc agcggcgcga
      601 tgttcctgcg gatccagccg ccctcggacg cgctgcccgc gaggctcgcc ttgtggccgt
      661 tggcgaacgc ggtggcgatc tcgagcagcg agcggatggt ggcgttgtcg agatcgcgcc
      721 cctcggccgg cttgccgccg ggcgcgaggc acgccccgca caccgccggc cagcgctcgt
      781 gcgcggcggc gtccatcgcc gccagcgagc gcgcccggtg ctcggggctg taccgctcgt
      841 gcgcgttcgt cgacacggcc caatggacga gctccgccag cgccgcggcc gggtcggccg
      901 gcaggccggc gatcatcttc ttcacttcgg cttcgttgtg gagcggatgg tcgtcacccg
      961 ccccgaaggc cctcgttatc atttttcgac tccccctgcg gcctggcgaa cagcaatatc
     1021 cgttcctgca ttcccgggcc gggcccggta cttttccacg ctcccttaca gcaccacgga
     1081 gacgtcgatg ctgccgctct tgccgacgac gctcacgccg acatcgtgct cgtgccgctc
     1141 gaaatcgagg tcgaccaccg cgtcgccgac gcgcaggttg gcgatctgca cgcgctgcat
     1201 ccacgcgggc aggatcgggt gcgtgaagcg gatgcgcggc gcttccggat ggaacgacag
     1261 cccgaggcag gcctgcagga cgtagaacac gctcgcgctc gcccaggcct gcggcgagca
     1321 cgccaccggg tacagcgtcg ggccttcgct cgggcggcgc acgaagccgc accacagctc
     1381 gggcagccgg tgcaggtcca tgaacaggct cgcctcgaac agcgcggtga gaatggcgat
     1441 ggtcttgtcc ttcatgccgt agcgggcgag gccgagcgcg atcagcgcgt tgtcgtgcgg
     1501 ccagatcgag ccgttgtggt aggacatcgg gttgtagcgc gcctcgcctt cggcgagggt
     1561 gcgcacgccc cagcccgaga acgactcgcg cgagaacagc gttcgcgccg tgcgctgggc
     1621 gtgctcgggg ctcgcgattc ccgaccacag cacctggccc gcgttcgacg agcgcacgac
     1681 gcagggacgt ttcctgccgt cgagcgcgat gacgtagctc tccagctcct cggaccagaa
     1741 cgcgcgctcg aagcgctcgc gcaggtcccc ggcctcgcgc cgcagcgtgt ccgcgcgctc
     1801 ggcggcgccg agcgcctgcg cgaggcgcgc ggcgcgcatc ttcgcgagat aggcgtagcc
     1861 ctgcacctcg gcgagcgcga tcggcccgtc ggcgagcgcg ccgtcgcggt ggaacacggc
     1921 gtcctcggag tccttccagc cctggttcgc gaggccctcg gcgctcttcc tctggtactc
     1981 gaggaaaccg tcgccgtcga cgtcgccgta ctcgcgcatc cagtcgagcg cgcgctcgac
     2041 gtgcggccag aggcgccgca cgaactccag gtctccggtg cgctggtagt aggcgccggc
     2101 gagcgcgacg aacagcggcg tcgagtcgat cgtgccgtag tagcagccga acgggatctc
     2161 gcccaggttc gcgagctcgc ccttccgcat ctcgtgcacg atcttgcccg gctcggcgtc
     2221 gcgctcgggg atgacgcggt cggcctgggt gcgcgcgagg aacgcgagca cacctcgcgc
     2281 gacggaagga tccgcccaca agtactccat ggccgtgagg atcccgtcgc ggccgaaggg
     2341 cgtgctgtac cagggaaccc ccgcgtacgg gtacggcccc tcggggttgc ccgcggtcag
     2401 catggtgagg tcggcgctgg agcggaacag ccagtggttg aactgctggt tcgtggtctc
     2461 gaccgacgcg ctgcgcgcgc gcacgctgcg cagcgtcgcc tcggtgcaca gcagcgcctt
     2521 cgcgaagtcc atcggcgcgc gctcgcgcgc gtccgggtcg caggtcacgg tgatctcgat
     2581 gccggccgtt gcgcgcggcg cgagcacgag ctcgtaggcg acgtgatcgg gcgcgatcgt
     2641 ctccggcggc ggcgagcagt cgaggtcggt gcgccggacg acgccgtcga gcccgcggta
     2701 gccgaggcgc acgcgcgccg gggagagctc gggtggcagc tcctggccgc ggcgctcgcg
     2761 cctgtagccg cgcacctcga agatgtcggc gtagtccgcg gcgaactcga gcgtcagcct
     2821 gagcgcgacg ggctgcgcgc cgtagttcgc gatctcgagg cgctcgtggc acgccgcgtc
     2881 ccacagcagc cgcgagcgcg cgaggtgcac cgtgcccttg agcacctcga gcttcgcatt
     2941 ttcgcccggg ccctcatgaa ggtccggcgt ggtgaggtcg cagtgcagct ggctgttgtc
     3001 ctgcttcacg ctcgagttga gcagcgtcgg cttctggccg ttgatgcgca gctcgtagtg
     3061 cgagaggaac ctcgtcccct ggtggtagat gccgtgctcg ccgacgccga cgcgcgggat
     3121 gtcgccgtag ccgtcgaaca ggccgaaggt ctcgccgtgc ttgagcacgc gcgtggggtc
     3181 gccgtgccgc gagtgggtcg cgaggacgta ccaccggtcc tcgatccgga tcgcctcttc
     3241 catcgcgagg ttcatcaacc gttctccttc tcgccgatac gactcgcctg cggctcgggc
     3301 ggctcgagca gccgccggta cacggacagg tattcgcgcg ccatgcgccg cgccgagaag
     3361 cgctcctcga acgcccggcg gcaggccgcg cggtccaggc ccggcatcct gccgatcgcg
     3421 gccgcggcgc tgcgttcgtc gtcgaccacg aaaccggtga cgccgttcac catgatctcg
     3481 ggcaccgagc cgcagcgcca ggcgagcacc ggcgtcccgc acgccatcga ctcgatcatc
     3541 accagtccga acggctcggg ccagtcgatc gggaacagca gggccagcgc ttccccgaga
     3601 aagcgcgcct tgtcggcctc gccgatctcg ccgacgaact cgacgccggg cccgtcgagc
     3661 atcgggcgga tcacctcgtc gaagtactgc aggtcgagct tctcgatctt ggccgcgatc
     3721 ttcagcggga tgccggccgc gcgcgcgagc cggatggcgc ggtcggggcg cttctccggc
     3781 gatatgcggc cgaggaaggc gaggtaggag ccggggcgcg cgctgaacgg atagagctgg
     3841 ggcggcaggc cgtgatggac gttggcgacc cagttcgccg acgccatcgg gaggcgttgc
     3901 gcgtgcgaga tcgacacgag cggcacctcg ccgaagcgcc ggtgcaccat ctgcatctcg
     3961 ggcacgtcga ggcggctgtg cagcgtcgtc acggagggca ccgcgagacg tcgcgcgaac
     4021 ggcagctgca gatagtccag atggaagtgg acgatgtcga actcgcgcac ggactcgaac
     4081 acctggtcca gcatgaccag gtggtacgcc atcgggtcgc ggcagcccgg gtcgaggcgc
     4141 aggccctggc gcgccatcgg ccgcagccgg gcgcgcgtgc gcgagtcggc gctggcgaac
     4201 agcgtcacct cgtggccctg cgcgaccagc tcttcggtga gataggagac gacgcgctcg
     4261 gtgcccccgt agagccgggg cggaacgctc tcatacaaag gggcgacgat cgcgacgcgc
     4321 aatggctggg cctcgggatt gacatcattg ggctgcgcgt gcgcgcgcct gcggcgggct
     4381 ctgcggccgg cctaatagga atgacaccgc cgcggcgcgg ggggttcctt ccggccctcg
     4441 cgcgcggcgc ggcggcggcg atgctacagt tcgccgattc cccaggagaa cgcgccgtgc
     4501 cccgatccgt gaccgcccga gtcgccattc ccgacatgat ctcgccgtcg tactttcccg
     4561 cgatcgccgc ggtcgagctc ggctacttcc gcgaggaggg aatcgacgcg acgctcgagc
     4621 tgctctaccc ggtgacgaag acctacgagg agctgcgcga agggcggctc gatttcgtcg
     4681 gcggcgccgc gcacgcgccg ctgtacgcgt tcccgggatg gaaaggctgc aagctgctcg
     4741 ccgcgctggc gcagaacatg tactggttcc tcgtagtccg caaggacctg tctcccaggc
     4801 gcggcgatct cgcggtcgtg aaaggcctgc ggatcggcgc ggcgccgggt cccgtcgacg
     4861 ggctcaggcg catgctgcgc gaggccggga tcgatcccga gcgcgaggtg aagatcggcc
     4921 cggtccccgg gtgggcgtcg gcgggcgcgt cgttcggcct catggccgcg cacgcgctcg
     4981 aggagggcaa gctcgacggc ttctgggcga acggcatggg ggccgaggtc gccgtgcgcc
     5041 gcggcgtcgg cgcgctggtg ctcgacgcgc gccgcggcga cggccccgcc ggatcgcagc
     5101 actacacgtt cccggccctg gtgacgacgc agaaatggat cgacgagaag cccgacgcgg
     5161 tcgccggcgc ggtgcgcgcg acggcgcgcg cgcaggccgc gctgcgcgcc gaccccgcgc
     5221 tcgcgacgaa ggcggcgagc atgttccccg aggccgagcg cgccctgatc gccgagctga
     5281 tccggcgcga cgcgccgttc tacgatcccg cgatcgagga gccggccgcg cgcgcgctca
     5341 acgacttcgc gcgcgacatc ggcattctcc gcggtgcccc gagctacgag gacaccgtcg
     5401 cggtgcgctt ccgcggcgcc tggaagccct agcgcgggca aaaaaaatcc cgcgccggtg
     5461 cgaaaggcgc gggacgggga acggaggcgc tcagccgccg ctcttggtgc tgctgctcga
     5521 gccggaaggc gagttgctcg cggtgtcgct cgagcccgag ccgctgctcg aggacgaggc
     5581 cgagggcgac gagctcgatc cgctcgactg gtcgctcgag gtggaaggcg agctggagct
     5641 cgagctcggc gtgctgctcg aggcgctgga gctcggcgag ctcgagctgc ccttgtcctt
     5701 gttcatcgcc aggctgccgg tcgacgagcc cgaggacgac gagcccgagg ggctcgaggg
     5761 cgacgaggac gccatgcccg aggagcccga ggagcccgac gagcccgact gggacaggcg
     5821 cgagtcgctg ccgcgatcgc aagccgagag tccgaaggtc gccgctatcg cggcggccac
     5881 ggcggtaaga aagaatcgtt tctgcagcat gtcgacaatc tcctgaagaa gaataatagg
     5941 gcgtgctcct gatcgaatca ggcaaccccg ctctcgcaat gcgtatgcca ggcccggacc
     6001 gaccgcaaaa gcgcgcttcg ctagtccatg cgacatcggc gtgccggaaa atttttcaaa
     6061 cgcccgtgtg gcatttaccg gcgccgaagc gtgctacgat tcgcggcccc ggcgagcgtc
     6121 gtccgggcac gacagcggag gcgccatgaa gcgcagcagc gatcgttttc tcaccacgca
     6181 taccggcagc cttccccgtc cggaagacct catccggacg atgtacgcga aggaagaggg
     6241 cgtgccggtc gaccgcccgg cgctcggcgc gcgcatccgc tccgccgtcg aggagacggt
     6301 gcgcaagcag gccgaggcgg gcatcgatct cgtcaacgac ggcgagatga gcaagccgag
     6361 ctacgccacc tacgtgaagg accggctgag cggcttcggc ggcaccggca acaccttcgt
     6421 ctaccaggat ctcgccgact tcccgagcct cgagaagcgc gtgttcggcg acccggggcg
     6481 ctcgcgccgc aagacgccgg cgtgcaacgc gccgatcggc gtgcgcgatc ccgaggccgc
     6541 ggccgccgac gcgcgcaacc tcaaggacgc ggccgcacgc gtgaagaccg aggggacgtt
     6601 catgagcgcg gcctctccgg gcgtggtgtc gctcttcttc cgcaacgatt actacaagac
     6661 ccaggaggaa tacctgttcg cgatcgccga cgcgatgcgc cacgagtacg agaccgtcgc
     6721 gaacgcgggc atcgtgctgc agatcgactg ccccgacctc gccatgggcc ggcacatcca
     6781 gtacgcgagc ctgccgctcg ccgagttccg caagaagatc cggctgcaca tcgaggcgct
     6841 caaccacgcg ctcgccaaca tcccgcccga gcggctgcgc atgcacctgt gctgggggaa
     6901 ctacgagggc ccgcaccact gcgacgtgcc gctcgccgac atcatcgacg tggtcttcgc
     6961 cgcgcgtccg gccgcgatct cgttcgaggc cgcgaacccg cgccatgcgc acgagtggac
     7021 gctgttcgag cgcgtcaagc tgcccgaggg caaggtgctc attcccggcg tcctcgaatc
     7081 caagaccaac ttcatcgagc atcccgagct cgtcgcgcag cgcatcgggc gctatgccag
     7141 gctcgtgggc ccggagaacg tgatcgccgg caccgactgc ggctacggca cctgggtggg
     7201 ccaggccgcg gtcgatccac aagtcgtgtg ggcgaagctc gccgcgatgg ccgagggcgc
     7261 gcgcatcgcg tcgaagcagt tcttcgggcg ataaaaaccc gcgccgcggt tgcccgcggc
     7321 gcggttccga tcggcattcc cgtaatagtt acgccctggc gctcgagtgg cgaaggttcg
     7381 ggtcgccggt cgagccgtag gggctcgacg acccgtccga cgggctcgac cacagggtgc
     7441 cgtcggtgtc gttctgcgcc agctggccgt cgttcgcgcc cggattgccg gagtcgctct
     7501 gcgccgaccg gctgtcgtcg ctcgggccgc tctgcagcga gggatcgtca cccggcgtcg
     7561 gcgtgctctg cgggcagttc gcgctcgcgc tgtcgtcgca gggcgggttc tgcgccagct
     7621 ggccgttgtc gcccggggcc gtgctttgcg cctgggtctg gctgtcgccg ctcatcgacg
     7681 cgcccgacga agcggacgaa tcgtgcgcca gcgcggcgag cggcgcgctc agcgcgccgg
     7741 cgatgatcgt ggcgagaagt gtcttcgatc gcatcaatgg tctcctgatc tagtacttgc
     7801 cgttggccga gtactgctga ttgctgctct ggctcgggta cgagttgctc tggctcgcgt
     7861 ggcggtcctg atcgtggtca cggtccttgt cgtggtcgcg gtccttgtcc ttgtcgcgtc
     7921 cgcgatgcct gccgttgtcg tgatcttcgt tctgcgcgta gcggtcgttc cgctcctggc
     7981 cgtacgcgcc cgccgagcgg tcgctcgagc cgagcgagcc ctgcaccgac gcgccgccga
     8041 tgttggcgct cgcggaaggc gactgggatc catacgagga attgttcttg tcgtcggccc
     8101 gtgcggcgag cggcgccgcg agggcgcctg cgagagcggc ggcaagaatg gtcttcgatc
     8161 gcattacaga atctcctttg agtaaagaat cggaatcgcc cggcgttccg ggcaaggaga
     8221 ttcacgcaac gcgcgtgccc gcttcaccga cgagcggacg tcggggcgcg acgacaggac
     8281 tactcggtcg cgaagcgcgc gatcagctgt ttcactttct gtgacggcgc gaggaactgg
     8341 cagccccagc gctgcgcgcg acggccgtcg gcgaactcga ccggccggga atggcgcacc
     8401 tcgagatcga ccgcgatcac gccctggtcg ccgctctcga tgcggcagcc ggtgatgcgg
     8461 gtgcccgcgg ccggcacgat gctcacgccg tgcaggagca ggccgatgcc ggcttcgctg
     8521 atgtccatga tctccgcctg cagcggcgcg ccggtgccga ccggaatgat gcaggcggcg
     8581 gggaagccct gcgggatttg cgcgcgcggg tgcgcgcggc gctgccgcgt gacgatcgcc
     8641 ctcggaaagc cgagacgcac cgacgcgggc gcgtggccgc tgggccgcgg gtcggtggac
     8701 gcgaactcga tctgcatgcc gccgaactcg gcgacgaagg tcgcgagcgg ccgctcgggc
     8761 agcgtcctcg acgccgattc gggcgccgga tcgaggacga tgtactggcg cccggcgtcg
     8821 agcagcgcga gccgccaggt cgagtgcggc cggcccgcgg cgtcgagcgc gaccagcgac
     8881 tgcttgcgga tgagcagcgc ctgcagcacg cgctcgacct cgtccgccga ggtggagacg
     8941 acgccgggag ccagcgtcag cgtattcgga tgcggtgtcg ccaagatcga gccctcagcc
     9001 gcggccgatg aacggcatct tggtggccat gatggtcatg aactgcacgt tggtgtcgag
     9061 cggaagcccg gccatgtaca gcaccgcgcg ccccacctcg tcgacctcca tcagcggctc
     9121 ggcggcgatc gtgccgttcg cctgcggaac gccgttcgcc atgcgcgagg cgaggtcggt
     9181 gcgcgcgttg ccgatgtcga tctgtccgca ggcgatgtcg tacttgcgcc cgtcgagcga
     9241 cgtcgagcgc gtgaggcccg tgatcgcgtg cttggtcgcc gtgtacggcg ccgagttcgg
     9301 ccgcggcacg tgcgcggaga tcgagccgtt gttgatgatc cggccgccgc gcggatcctg
     9361 gctcttcatg atccggatcg cctcctgcgt gcacaggaac gcgccggtga ggttggtgtc
     9421 gacgacggcc ttccactgct cgacggtcag gtcctcgagc atgacgctgc ccggcgtgcc
     9481 gatcccggcg ttgttgaaga gcaggtccag gcgtcccccg aaacgctcgc gcgtcttcgc
     9541 gaacagcgcg cgcaccgacg cgggatcgcc gacgtcggtc tgcaccgcga gcgcgcgcga
     9601 cgcgccggcg cccgcctcgg cgagcgcttg ctcgagcttg tccctgcggc ggccggcgag
     9661 cacgacgctc cagccggcgc cgaggagcgc gagcgccgcc gacttgccga tgccggtgcc
     9721 gccgccggtg accactgcga ccttcgatgc gttcatgggt tccttcccgt cgttggtggg
     9781 catgtctcag gcggcgccgg ggcctttggt catgcacgcg gcgacgagct gacgcacgcc
     9841 cagcgcgttg gcatcgacga agcggcagcc cgagcgctgc gtatacggac cctcgctcag
     9901 cagcgcgacc tgcgagtagc gcacctcgag gtcgaagatg ctcggcgcca tcctcagccc
     9961 cgtgaccacg cagcccctga ggatcgtccc gggctcgagg ctgatgtcgg caggatacag
    10021 caggccgacg cccccggcgc tgatgtcgat cacctgggcg tcgaacggca tgtcgccgct
    10081 cgcgtcggcc aggcagtgga gcgggggcga gatggcgacg cgcgcgtcgg cgcggcgcgc
    10141 gtaggtcgcg agcacgtcgg gaaagtcggc gcggatcagc ggacggccgt cgtgctccat
    10201 gcgcgcggga ttgctcgcgg cgaactcgaa atagcgctcg cccacgttgg cgcagaagat
    10261 ggcgcgcagg cgcgacacca gcgcctcgtt cgcttcctcg tcgtcgcacg cgtcgaggac
    10321 gatgtggcgc aggcccggat cgatctgccg cagccgcgac acgaactgca gcgtgccgcg
    10381 cgccacgttg gagagcagcg gcgcgccgcg cgaggcgagc agctcgagga cccgcgcgat
    10441 ctcctgcggc gcacgcgtga tgtgctcggc cggcagcgcc tcgctcaccg gggtcccgcg
    10501 atgtcgcgcg cgcgctgcac ctcgtcggcg acgcggctcg ccgactcggc ggcgcgccgg
    10561 cgcgcgtcgg cggcgagccc ttcgtcgagc agcgccgacg cgtcgacctg cgccgcgagc
    10621 ttggcgcggg cgccgggaaa ggcgtcgagc tcgcgcgcga gcgcgtcggc ggcgacgccg
    10681 taatcgaagt agcgcttctc gaggtcgccc ttgatctgga gcgcgcggct gaggaagtcc
    10741 ggcgtgcggc ggttggcgtg gttcatgtgc tgcacgagca accgggcgcc ttcgtcgacc
    10801 cgctggcggc cgcgctcctc gcgggcgagc tcgcgcagga tctggcgcgc cgacagcgcg
    10861 tactcctcgg ccgcgtaagc gagcgcgcgc ccgggcgccg tcctgacgcg ctcgaagtcg
    10921 gcgaggcgcg cgtcgagccc ggccgcgtcc gcgtcgagct gtgccagccc ggcgccgctg
    10981 ccgggatgcg cgcgcaggcc gtcgcgcagg ccggcgctcg cctcctgcac caggtcggaa
    11041 gcggcgcggc gcagctcggc gcgctggtgc gcgctccagg ccccccagcc cgcggcggcc
    11101 gcgaccgccg cggccagcac caccagcacg acgctcttcg atcccgaggt cgccatggcg
    11161 gcggttatac agtcatccgc gggcgccgac aaccgagaag ttgcgcgagc gccgcccgag
    11221 gcggcaaact agccgcgcga cgaggaggag cgagcttgaa ggccatcgtg atccgagagt
    11281 tcgggccgcc cgaggtgatg cggctcgagg acgtgccgac gcccgagccc ggcccgggcg
    11341 aggtcgtggt gcgcgtgcac gccgtgtcgg tgaaccgcac gctggacctg atcctgcgcg
    11401 cgggcgcgta tgcgctgccg atcgagctgc cgcacgtgct cggcgtcgac ccggccggcg
    11461 tggtgagcgc cg
//
LOCUS       DATDMW010000022         3433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1622565, whole genome shotgun sequence.
ACCESSION   DATDMW010000022 DATDMW010000000
VERSION     DATDMW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622565"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..306)
                     /locus_tag="VI319_00710"
     CDS             complement(37..306)
                     /locus_tag="VI319_00710"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862394.1"
                     /translation="MLCLTSRDRPIPPNPPRNGVFAPVTIDGGGSGIGGDGSGGDSGS
                     GISGQQAYVKASNTDSNDEFGFAIALSGDGSTMAVGAYGEASTFQ"
     gene            447..620
                     /locus_tag="VI319_00715"
     CDS             447..620
                     /locus_tag="VI319_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862395.1"
                     /translation="MVRLAQVVRAPPVQRDEVPRGTAVIELLEARVRITVASDTDRAV
                     LAMVLDVLDRRGR"
     gene            617..958
                     /gene="tnpB"
                     /locus_tag="VI319_00720"
     CDS             617..958
                     /gene="tnpB"
                     /locus_tag="VI319_00720"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY6862396.1"
                     /translation="MIPHGVEVYVALDPIDLRWGFDRLAGVVTERLGRGARSGALFVF
                     FGRRRTALKVLFFDGTGMCIVYKRLDAGAFRIPEPVVTGDAARLINERAFEDLLAGID
                     LESVSRRHRVH"
     gene            1090..3135
                     /locus_tag="VI319_00725"
     CDS             1090..3135
                     /locus_tag="VI319_00725"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY6862397.1"
                     /translation="MSGSTSPNDPAVTARLTELEATVVAQRAALEAERELRAAAEAER
                     DRLREAYEALKIQVELAHRRLVIAKAERVDTTQLELEFAATLAELDKLAGLVEPETDA
                     TGDEGGAAGHRPKRPRGRRCDLHKVVGEERIELPDPIYEGRFERIGAEEETAKLKWRR
                     GGLVRVVIARLKYRTTPPAARMVDAIPSAADASSATGAAEVEVASATMTVPPEDATSS
                     TVEVANVDATTSANASPVDARPTQLAISSAAPATTPDAPAPVAAVPAIADPAADSNTS
                     KPTIVTAAMPPQLLPGSYALPSLLAHIASDKFCDGLPLHRQEDRFARLGATIHRSTMA
                     RWLEEVGAIMGATLVEAMRKEAMATAFCIATDATGVLVQPDPRPDKKRQPCRRAHFFV
                     QIADADHVFFEYTPVETSKVVSELFRDFSGFIQADAKSVYDVLYRLPHERDLFDDGSP
                     DLAIRHEVGCWSHARTKQWEAAVATQDPVAREALARIMRMFQLERRWKDRSPDERKAL
                     RDRHLRPHVVAFFEFAEEAYERVKHQRGLLRSALGYCVRQKDALMRFLEDGRLEMTNN
                     HSERQLRKIACGRKAWLFVGSDDHGQAAGNLLTLIASARLHKLDPELYMRDVFRVLPH
                     WPRERYLELAPRYWSATRARLDPNELEQEIAPLKIPELPLPMAHTAVPLPADLAPAP"
     gene            3242..>3433
                     /locus_tag="VI319_00730"
     CDS             3242..>3433
                     /locus_tag="VI319_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862398.1"
                     /translation="MPALLRLYLLALAKDRSPDAQLISRTLAPRSGKKRDRHWLLRHV
                     ERLCDDAGVPVVCTQSLRGL"
BASE COUNT          564 a         1148 c         1171 g          550 t
ORIGIN      
        1 gtgatctggc gcggtcgctg atcctgcccg acgggctcac tgaaaagtgc tcgcctcgcc
       61 gtaggcgccg accgccattg tcgagccatc acccgacagt gcaatcgcga acccgaactc
      121 gtcgttcgaa tcggtgttcg acgccttcac gtaggcctgt tgccccgaga tgcccgagcc
      181 gctgtctccc ccggagccat ccccgccgat gccgctgcca ccgccatcga tcgtaaccgg
      241 tgcaaatacc ccgttccgcg gcgggtttgg cgggatcgga cgatcgcggg atgtcagaca
      301 cagcaacgaa gtggcgtcag cgggtcgccg gctggcgagc cagcggcgag accgcggaga
      361 catacagcac ggggcgagga ttcaccgcgg gtaccctgcg atggtgggcg tcccgtctcc
      421 gtcgagagga cacgatcgct ggcccggtgg tgcgcttggc ccaggtggta cgggcgccgc
      481 ccgtacagcg ggatgaggtg ccgcgcggca cggccgtgat cgagttgctc gaggcacggg
      541 tgcggatcac ggtggcatcg gacacggacc gcgcggtgct ggcgatggtc ctcgacgtgc
      601 tcgatcggag gggacggtga tcccgcacgg cgtggaggtg tacgttgcgc tggacccgat
      661 tgacctacgg tggggattcg atcggttggc gggtgtggtg accgagcgac tgggccgcgg
      721 ggcgcgcagc ggagcgctgt tcgtattctt cgggcggcgg cgcaccgcat tgaaggttct
      781 attcttcgac ggcactggaa tgtgtatcgt ctacaagcgg ctcgacgctg gggcgtttcg
      841 gatcccggag ccggtggtga ccggcgatgc tgctcgtctg atcaacgagc gcgcgttcga
      901 ggacctgctc gcgggcatcg acctggagtc ggtgtcgcga cgtcatcgcg tgcactgacc
      961 gacgacgctg ctcggcgtga ccgtcgacga acagcgagca agttccggac caggaatctc
     1021 gcgctctcgc gctcgatttc gatctcgcgc atcttgcgcg cgccgatcgc aggatcatga
     1081 tgcgcgagcg tgagcggctc gacgagtccg aacgatccgg cggtcacggc gcggctcacc
     1141 gagctcgagg cgaccgtggt ggcgcagcgg gccgcgctcg aagccgagcg cgagctacgc
     1201 gccgccgccg aggccgagcg ggatcgactg cgcgaagcct acgaggcgct caagatccag
     1261 gtcgagctcg cccaccggcg cctggtgatc gcgaaggccg agcgcgtcga tacgacccag
     1321 ctggagctgg agttcgccgc cacgctcgcc gagctcgaca agctcgcggg cttggtcgag
     1381 cccgagacgg atgccacggg tgacgagggc ggcgcagcag gccacaggcc gaagcggccc
     1441 cgtggccggc ggtgcgatct gcacaaggtg gtcggcgaag agcggatcga gctcccggat
     1501 ccgatctacg agggcaggtt cgagcgcatc ggcgccgagg aggaaaccgc gaagctcaag
     1561 tggcgacgcg gcggcctggt tcgggtcgtg atcgctcgcc tgaagtaccg aacgacacca
     1621 ccggcagcgc gcatggtcga cgcgatcccg tcggcagccg atgcgtcctc agccaccggc
     1681 gccgcggagg tcgaggtagc ctcggcaacc atgaccgtac cgccggagga cgcgaccagt
     1741 tcgaccgtcg aggtggcaaa cgtcgacgcg acgacatccg cgaatgcatc gccggtcgat
     1801 gctcggccga cgcagctcgc gatctcgtcg gctgcgcctg ctaccacgcc tgatgccccc
     1861 gcgccggttg ccgcggtacc tgcgatcgcg gatccggccg ctgactcgaa cacctccaag
     1921 ccgacgatcg tgaccgcggc gatgccgccg cagctactgc ccggctcgta cgcgttgcct
     1981 tcgctgctgg cgcacattgc gagcgacaag ttctgcgacg gcctcccgct gcaccggcag
     2041 gaagaccggt ttgcacggct cggcgcgacg atccatcgca gcacgatggc gcgctggctc
     2101 gaagaggtcg gcgcgatcat gggagcgacg ctcgtcgagg cgatgcgcaa ggaggcgatg
     2161 gcgaccgcgt tctgcatcgc gaccgacgcc accggtgtgc tcgtccagcc ggacccgcgg
     2221 cccgacaaga agcgtcagcc atgccgtcgc gcgcacttct tcgtccagat cgccgacgcc
     2281 gatcatgtgt tcttcgagta cacgcctgtt gagaccagca aagtggtcag cgagctgttc
     2341 cgcgacttct ctggcttcat ccaggccgat gccaagagcg tctacgacgt cctgtatcga
     2401 cttccacacg aacgagacct cttcgacgat ggctcgcccg acctcgcgat tcgccacgag
     2461 gttgggtgct ggagccatgc aagaaccaag cagtgggaag ccgctgtcgc gacgcaagac
     2521 ccggttgcgc gcgaggccct cgcgcgcatc atgcgcatgt tccagctcga acggcgctgg
     2581 aaggaccgca gtcccgacga gcgcaaggcg ctgcgcgatc gccatctccg gccgcacgtc
     2641 gtcgcgttct tcgagttcgc cgaggaggcc tacgaacggg tgaagcacca gcgcggcttg
     2701 ctgcgctcgg cgctcggcta ctgcgtgcga cagaaggatg cgctgatgcg cttcctcgag
     2761 gacggtcgtc tcgaaatgac gaacaaccat tcagagcgcc aactcaggaa gatcgcctgt
     2821 ggccgaaagg cgtggttgtt tgtcggcagt gatgatcacg gccaggccgc cggcaacctg
     2881 ctcacgttga tcgcttccgc gcgtctgcat aagctcgacc ccgaactcta tatgcgcgac
     2941 gtcttccgcg tgctgccgca ctggccacgc gagcgctatc tcgagctcgc tccacgctac
     3001 tggagcgcta cgcgcgctcg cctcgatccc aacgaactcg agcaggagat cgctccgctg
     3061 aagatccccg agctgccgtt gcccatggcg cacacggcgg tacccctgcc ggctgatctg
     3121 gccccggcgc cgtaggcgcc tgccacatcg cgcggcggcc ttcaagaacg gggatcttgc
     3181 accggttacg ctgctctgga tcgagttcgg caagacgaag cgatcgcggc gcacgctgga
     3241 ggtgccggcg ctgctccggc tctacctcct ggcgctcgcc aaggatcgct cgcctgatgc
     3301 ccagctgatc agccggacgc tcgctccgcg cagcggcaag aagcgggatc gccactggct
     3361 gctccgtcac gtggagcgcc tgtgcgatga cgctggcgtg ccggtcgtct gcacacaatc
     3421 gctgcgcgga ctc
//
LOCUS       DATDMW010000023         3772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_626329, whole genome shotgun sequence.
ACCESSION   DATDMW010000023 DATDMW010000000
VERSION     DATDMW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626329"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2551)
                     /locus_tag="VI319_00735"
     CDS             complement(<1..2551)
                     /locus_tag="VI319_00735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6862399.1"
                     /translation="MGIAGIPEDYSGRRRTAAALPPEAGGPARLRAAQGFSMTLAIAP
                     GHYLAPLLAPRSVALVGASERPDSLGRVVYENLLGGSFKGELFAVNPNHASVLGRPAF
                     ASLQAIGQAIDLAVICAPPQAVPAILEGARGRLRAAAILSGVPDAAAGAERRWRREVC
                     DRARAAGVRLLGPQSFGIMRTSLGLNATYGAIAALPGRLTLISQSGAIAMALLDFART
                     AGIGFASVIALGSGDDVYTGELLEFALADPDTDGILLYLETVRDARRFMSALRAAART
                     KPVVVLKAGRGDAALAAPGAPSPDRVFDAAARRAGTVRVENYTQLFATTRVLAAGGVP
                     RGNRLAILTNGRGPGLLAADRARSAAVALASLTRETKAALTRLLPAGSRLENPVDLRG
                     EAPPERFAAALRLVLDDANVDAVLVMHVATPAAPPTDAARAVASAAKGATKPLFAAWL
                     GAVARPEAGAALEAGGLANFYTPENAVEAFSFLAAYRRNQLWLLEAPPPQAEVARPDI
                     AAAERIHLRARSRGTSVLAPRQARALLGAFGISLAPAAAGRRARAHRAVDVGVYRDDV
                     FGPVITLRTAHATGAPEVMLPPLNRSLAGDLVDAVCGPLPAQEREALVALLLAISTLV
                     CMLPWLLQIDLPAVAFSAGRARPPGACVTIDLERGPGVRGYRHMAIHPYPVELETTLA
                     IAGGRTLRVRPIRPEDAAAERAFVAGLSDESRYRRFMHHLHDLTPQMLARFTQVDYDR
                     ELALVALDGRPRAEKIVAVARYVSNPDGESAEFAIVVADALHGRGLGRGLVRLLISCA
                     RRRGFRRLVGNVLAANGPMLKMIAGLGFETHPDPSDREQVIATLELNKAHEA"
     gene            2585..2658
                     /locus_tag="VI319_00740"
     tRNA            2585..2658
                     /locus_tag="VI319_00740"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2619..2621,aa:Ile,seq:cat)
     gene            2968..>3772
                     /locus_tag="VI319_00745"
     CDS             2968..>3772
                     /locus_tag="VI319_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6862400.1"
                     /translation="MFSLRIALGAVTALLLTGCMVGPNFQRPEAPATARYTETPLPAE
                     TASAPGSGGAAQRFVPGAELPGEWWSLYRSEPLQRLIQEALAANPTLAAARATLRQAQ
                     ENLAARTGALLYPSVNANASATREKISNAGFGASGASLFNLYNASVDVSYMLDLFGGN
                     RRQLESLQAAVDYQDYQLAGAHLALTANIVTAAVQEASLRAQIQATREVEAAQQQILD
                     VVEKQLKLGAVARADVLAQRAQLAQTRATLPALEKALAQARNTLAALVGR"
BASE COUNT          580 a         1350 c         1333 g          509 t
ORIGIN      
        1 acgcttcgtg cgctttgttc agctccaacg tcgcgatcac ctgctcgcga tcgctcggat
       61 cggggtgcgt ttcgaatccg agtccggcga tcatcttcag catcggaccg ttggctgcga
      121 gcacgttgcc caccagacgc cggaagccgc gccggcgcgc gcagcttatc agcagccgca
      181 caagcccccg gccgagcccg cgcccgtgca gcgcgtcggc caccacgatc gcgaactcgg
      241 cgctctcgcc gtcgggattc gacacgtagc gcgccaccgc gacgatcttc tccgcgcgcg
      301 gcctcccgtc gagcgccacg agcgcgagct cgcggtcgta atccacctgg gtgaaccgcg
      361 ccagcatctg cggcgtgaga tcgtgcagat gatgcatgaa gcggcgatac cggctttcgt
      421 cggacagccc ggcgacgaag gcgcgctccg cggccgcgtc ctccggacga atgggacgca
      481 cgcgcaacgt gcgcccaccc gcgatcgcga gcgtcgtttc gagctcgacg ggatacgggt
      541 ggatcgccat gtggcgatag ccgcgcacac cgggaccgcg ctccaggtcg atcgtcacac
      601 acgcgcccgg gggccgcgct ctgcccgctg agaaggcgac cgccggcaga tcgatctgga
      661 gcagccaggg cagcatgcac acgagcgtgg aaatggcgag cagcagcgcc accagcgcct
      721 cgcgctcttg agccggcagc ggaccgcaca ccgcgtcgac gaggtcgccc gcgaggctgc
      781 ggttgagcgg cggcagcatg acctccgggg cgccggtcgc atgcgcggtg cgcagcgtga
      841 tcaccggccc gaatacgtcg tcccggtaca cgccgacgtc gacggctcga tgcgcgcgag
      901 cccgacgccc cgccgccgcc ggcgccagtg aaatgccgaa cgcgccgagc agcgcgcgcg
      961 cctggcgcgg ggcgagcacg ctcgtaccgc ggctccgagc gcgcaggtga atgcgctcgg
     1021 cggccgcgat gtcggggcgc gcaacctccg cctgcggcgg gggcgcttcc agcagccaga
     1081 gctggttgcg ccggtacgcg gcgagaaagg agaacgcctc caccgcgttc tccggcgtgt
     1141 agaagttcgc gagtccaccg gcttccagcg cggcaccggc ctcgggccga gcgacggcgc
     1201 cgagccatgc cgcgaacagc ggcttggtcg cacccttggc cgcggaagcc acggcacgag
     1261 ccgcgtcggt gggcggcgcg gccggcgtcg cgacgtgcat gaccagtacg gcgtccacat
     1321 tggcgtcgtc gaggaccagg cggagcgcgg cagcgaaacg ctcgggcggc gcctcgccgc
     1381 gcaggtcgac agggttttcc aggcgcgacc ccgccggcag caggcgggtc agcgcagcct
     1441 tggtctcgcg cgtgaggctc gccagcgcca cggcagcgct tcgcgcccgg tcggcggcga
     1501 gcaatccggg tccccggccg ttggtaagga tcgcaagtcg attgccgcgc ggtacgcctc
     1561 ccgcggcgag cactcgggtg gtggcgaaaa gctgcgtgta gttctcgacc cgcacggttc
     1621 ctgcgcgtct cgccgccgcg tcgaagaccc gatccggcga cggtgcgccg ggtgcggcga
     1681 gcgcggcgtc gccacggccg gccttgagca ccaccaccgg cttggtgcgg gctgccgcgc
     1741 gcaaggcgga catgaagcgg cgcgcgtcgc gcaccgtctc gaggtagagc aggatgccgt
     1801 cggtgtccgg atcggcgagc gcgaactcga gcagctctcc ggtgtatacg tcgtcgcccg
     1861 atccgagcgc gatcaccgaa gcgaagccga tgcctgccgt gcgcgcgaaa tcgagcagcg
     1921 ccatcgcgat cgcaccggac tgcgaaatga gcgtgagcct tccgggcaac gctgcgatcg
     1981 cgccatacgt ggcgttgagc ccgagggacg tgcgcatgat cccgaagctc tgagggccga
     2041 gcaggcgcac gcccgccgcg cgcgcgcgat cgcacacttc ccgccgccag cgccgctcgg
     2101 cgcccgccgc cgcgtcgggt acgcccgaca ggatcgcggc cgcgcgcagc cgtccgcgcg
     2161 cgccttcgag gatcgccgga accgcctgcg gcggcgcaca gatcacggcc aggtcgatcg
     2221 cttggccgat cgcctgcagc gacgcgaacg ccgggcgccc cagcacgctc gcgtggttgg
     2281 gattcacggc gaagagctcg cccttgaacg agccgccgag cagattttcg tagacgacgc
     2341 gcccgagcga atcggggcgc tccgacgcgc cgacgagcgc gaccgaccgc ggcgcgagca
     2401 acggcgcgag atagtgcccg ggagcgatcg cgagcgtcat cgagaagcct tgggctgcgc
     2461 ggagtctagc aggcccgcca gcctccggcg gcagcgcagc ggcagtgcga cggcgtccgc
     2521 tataatcctc cgggattccg gcgatgccca ccgaagtccg ctgcacgagc ttgcatcgac
     2581 ccgcgggcct gtagctcagt cggttagagc agaggactca taatcctttg gtcgctggtt
     2641 cgagtccagc cgggcccact ctccccaaag cgtgcgcgtc acggcgctac tggttggtgt
     2701 cgcgatcgac cctgtagaat tcgccggtgt ccccgcctgc gttggcaggg gcctgcgtcg
     2761 attcgctcga gtccgcgccc tgccgtttcg gtggcaagcg cggaacatcg cgccgtcgct
     2821 ccggtcagga caatgctgag cccctgcacc agcgacgccg tcgacgcgct tgtaaaaatt
     2881 gcaacgatcc gacgcacgag ccacgcgccg gaagcccttc gagcggctcc cgaagagaca
     2941 gcgcatcaag aagaggagcg attggacgtg ttctcgctgc gtatcgcgct gggggcggtg
     3001 accgccttgc tgctgaccgg ctgcatggtg ggcccgaact tccagcgacc cgaggcgccg
     3061 gcgacggccc ggtacaccga aacaccgctg cccgcagaga cggcttccgc gcccggcagc
     3121 ggcggcgcgg cgcagcgctt cgtgcccggc gccgaactgc cgggagagtg gtggtcgctg
     3181 taccgttcgg agccgctcca gcgcctcatc caggaggcgc tcgccgcgaa tccgacgctc
     3241 gcggcggcgc gcgccacgct gcgccaggcg caggagaacc tcgccgcccg gacgggcgcg
     3301 ctgctctatc cctcggtgaa cgccaatgct tccgcgacgc gcgagaagat ctcgaacgcc
     3361 gggttcgggg cgtcgggggc atcgctgttc aatctttaca atgcctccgt cgacgtgtcg
     3421 tacatgctcg atctcttcgg cggcaatcgt cggcagctcg agtcgctgca ggctgcggtc
     3481 gactaccagg actaccagct cgcaggcgcg catctcgcgc tgaccgcgaa catcgtgacc
     3541 gccgccgtcc aggaagcgtc gctgcgcgcg cagatccagg cgacgcggga ggtcgaggcg
     3601 gcgcagcagc agatcctcga cgtggtcgaa aagcagctca agctcggtgc cgtggctcgt
     3661 gccgacgtgc tcgcgcagcg ggcgcagctc gcgcagacgc gcgcgacgct gcccgcgctg
     3721 gagaaggcgc tcgcacaggc gaggaacacc ctggcggcgc tcgtcgggcg ct
//
LOCUS       DATDMW010000024         3991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_377983, whole genome shotgun sequence.
ACCESSION   DATDMW010000024 DATDMW010000000
VERSION     DATDMW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377983"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..487)
                     /gene="dksA"
                     /locus_tag="VI319_00750"
     CDS             complement(68..487)
                     /gene="dksA"
                     /locus_tag="VI319_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY6862401.1"
                     /translation="MQLQPKTFTAYVPKKGEEYMNPRQREHFKAMLEALKVELSQDIA
                     GTVQAMQHEATLFADPNDRATQESDMALELRNRDRERKLIKKIEETIAKIEKDDYGYC
                     ENCGVEIGLKRLEARPTATLCIDCKTLDEMRERQVAK"
     gene            618..694
                     /locus_tag="VI319_00755"
     tRNA            618..694
                     /locus_tag="VI319_00755"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            697..773
                     /locus_tag="VI319_00760"
     tRNA            697..773
                     /locus_tag="VI319_00760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:Arg,seq:tct)
     gene            860..1564
                     /locus_tag="VI319_00765"
     CDS             860..1564
                     /locus_tag="VI319_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY6862402.1"
                     /translation="MIDVYTWPTPNGHKVHIALEELGLEHRIRPVNIRTGEQFKPEFL
                     AVSPNNKIPAIVDSDGPDGKPIPVFESGAILIYLAGKTGKLLPKKDRDKYVALQWLMF
                     QMGSIGPMMGQANHFRSNAAPERIEYAINRYSSEVKRLHGVMERRLSEAPWFAGSEYT
                     IADIAIFPWLRASERNGIVWSEFPKVKAWFDRINARPAVQKALQVLAEVNTSPPGHYD
                     AKAREVLFGATQYQKH"
     gene            1589..1978
                     /locus_tag="VI319_00770"
     CDS             1589..1978
                     /locus_tag="VI319_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862403.1"
                     /translation="MHIGGYDVSGLIYAAIVFIVALFLIRAHRDPNNPFDLFDLLKIN
                     GHVSKVAVAFLTTLFTSSWYVVHEALEKQLTDTAFAAYLAAWVAPIVAAILKGDAGIS
                     PAASDAPVPSPAIPAPVALPGVKPPAV"
     gene            1994..2069
                     /locus_tag="VI319_00775"
     tRNA            1994..2069
                     /locus_tag="VI319_00775"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2027..2029,aa:His,seq:gtg)
     gene            complement(2079..3548)
                     /locus_tag="VI319_00780"
     CDS             complement(2079..3548)
                     /locus_tag="VI319_00780"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY6862404.1"
                     /translation="MNDMAKPGAYNDYDRPHADLREFLARVERAGELARIPRAHWNLE
                     MGTLAEIVNQHPAGSRAVLFDEIPDYPAGMRVLSGATNSMKRLAVTLGFPAPSHPLDV
                     VRAYRDRMQTHRPIRPEVVKKGSVLENVQRDDAVNVLKFPVPFLHEQDGGRYIGTDDL
                     VIMRDPDGGWVNCGTYRVMAHDAKRVGVWMSPGKHGRQIREKYFRQGKPCPVLVSCGH
                     DPLLFLAGGNELRFGLSEYDYAGGHRGAPIEVLESELHGLPMPAHAEIVLEGEMHPGE
                     TAPEGPFGEFTGYYAGGQSEQPVIRVQRVYHRNDPILTMATPMRPPSDFSYSKCVMKA
                     GMIWDEVERAGLSGVKGVWCHEAGGARMFNVIAIKQAYAGHARQAGMLAASCQSGAYL
                     GRFVVVVDDDVDPTDMFDVMWAMCTRCDPAQDIEVVRRMWSGPLDPRIPRGTAWNSRA
                     IIDACRPYEMLAEFPPVARATPELRRKVEAKFREILAKL"
     gene            complement(3545..>3991)
                     /locus_tag="VI319_00785"
     CDS             complement(3545..>3991)
                     /locus_tag="VI319_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY6862405.1"
                     /translation="AVARRNVAAYRLKSRVRLVRSDLFRDFRAGPYDVIVANPPYVDA
                     RAMRSVPAEYRREPRLALAGGGDGLAFVRRILHEARRFLRPRGLLVVEIGRHRRELER
                     AFPKTPFTWLETSAGDGLVFLLHREDLPPAPEEVQSRREPAKRAAR"
BASE COUNT          639 a         1344 c         1315 g          693 t
ORIGIN      
        1 aaaagggcgc cgatggcgcc ctttttattt tgtcatcctg aggagcgaag cgacgaagga
       61 tctccttcta tttcgcgact tgcctttccc gcatctcgtc gagcgtcttg cagtcgatgc
      121 acagcgtcgc cgtcgggcgc gcctcgaggc gcttcaggcc gatctccacg ccgcagttct
      181 cgcagtagcc gtagtcgtcc ttctcgatct tggcgatcgt ctcctcgatc ttcttgatca
      241 gcttgcgctc gcggtcgcgg ttgcgcagct cgagcgccat gtcggattcc tgcgtggccc
      301 gatcgttggg atcggcgaag agcgtcgcct cgtgctgcat cgcctgcacc gtcccggcga
      361 tgtcctgcga cagctcgacc ttcagcgcct cgagcatggc cttgaaatgc tcgcgctggc
      421 gcggattcat gtactcctcg cctttcttgg gcacgtaggc ggtaaacgtc ttaggctgca
      481 gctgcaaatt cgtgctccac tttcgacgaa aaacaatgcg tcttgatacc agaaatctgc
      541 agggggagca agcttgattt cgcagcaaaa atcgcgcgtt gcaagggcct ctgcgcaggc
      601 gtagtatcct gggccctcgg ggtgtagcgc agcctggtag cgcgcctgct ttgggagcag
      661 gatgtcggga gttcgaatct ctccaccccg accaaggtgc ccgtagctca accggataga
      721 gcaccagcct tctaagctgg gggttgtggg ttcgagtccc gccgggcgcg ccacctttct
      781 gccgcggtcg cattttgcga ttaatatccg atgcgatcgt catagggcgc cgaataccac
      841 gccggaggca gagataacca tgatcgacgt ctacacctgg ccgacgccga acggccacaa
      901 ggtccacatc gcgctcgagg agctcggcct ggagcacagg atcaggcccg tcaacatccg
      961 caccggcgag cagttcaagc ccgagttcct cgccgtcagc cccaacaaca agatcccggc
     1021 gatcgtcgac tccgacggtc ccgacggcaa gccgatcccg gtcttcgagt cgggcgcgat
     1081 cctgatctac ctcgccggga agacgggcaa gctgcttccg aaaaaagacc gcgacaagta
     1141 cgttgcgctg cagtggctga tgttccagat gggctcgatc ggcccgatga tggggcaggc
     1201 caaccacttc cgcagcaacg cggcgcccga gcgcatcgag tacgcgatca accgctattc
     1261 gagcgaggtg aagcgcctgc acggcgtgat ggagcggcgc ctgtccgagg cgccatggtt
     1321 cgccggctcc gaatacacga tcgccgacat cgcgatcttc ccgtggctgc gcgcgagcga
     1381 gcgcaacggc atcgtctggt ccgagttccc gaaagtgaag gcgtggttcg accgcatcaa
     1441 cgcgcggccc gcggtgcaga aggcgctgca ggtgctcgcc gaggtgaaca cctcgccgcc
     1501 cggtcactac gacgccaagg cgcgcgaggt gctgttcggg gcgacccaat accagaagca
     1561 ttgagcggcg tcatcgagga ggggaaccat gcacatcggc ggctacgacg tcagcggcct
     1621 gatctacgct gcgatcgttt tcatcgtcgc gctcttcctg atccgggccc accgcgatcc
     1681 gaacaacccg ttcgacctgt tcgacctgct gaagatcaac ggccacgtct cgaaggtggc
     1741 cgtagcgttc ctcacgacgc tcttcacctc ctcgtggtac gtcgtgcacg aggcgctgga
     1801 gaagcagctg accgatacgg ctttcgcggc gtatctcgcg gcgtgggtgg cgccgatcgt
     1861 cgccgccatc ctgaagggcg acgcaggcat ttccccggcc gcgtcggacg ctcccgtgcc
     1921 gtcgccggcg atcccggcgc ccgtcgcgct ccccggagtc aaaccccccg cggtataatg
     1981 ccgttccccg acggtggccg tagctcagct ggtagagtcc cggattgtga ttccggttgt
     2041 cgtgggttcg agtcccatcg gccaccccac ctcgcttcct acagcttcgc caggatctcc
     2101 ctgaacttcg cttcgacctt gcgccgcagc tcgggcgtgg cgcgcgcgac gggcgggaac
     2161 tcggcgagca tctcgtaggg acggcaagca tcgatgatgg cgcgcgagtt ccacgcggtg
     2221 ccgcgcggaa tgcgcgggtc gagcggcccg ctccacatgc ggcgcacgac ctcgatgtcc
     2281 tgcgccggat cgcagcgcgt gcacatcgcc cacatcacgt cgaacatgtc ggtcggatcg
     2341 acgtcgtcgt cgacgacgac cacgaagcgc ccgaggtaag cgccggactg gcagctcgcg
     2401 gccagcatcc cggcctggcg cgcgtggccg gcgtaggcct gcttgattgc gatgacattg
     2461 aacatgcgcg cgccgcccgc ctcgtggcac cacacgccct tcacgccgga gaggcccgcg
     2521 cgctcgacct cgtcccagat cattcccgcc ttcatgacgc acttgctgta ggagaaatcg
     2581 gacggcgggc gcatcggcgt cgccatggtg aggatggggt cgttgcggtg gtagacgcgc
     2641 tgcacgcgga tcaccggctg ctcgctctgg ccgccggcgt aatagccggt gaactcgccg
     2701 aaaggacctt cgggcgcggt ctcgcccggg tgcatctcgc cctcgagcac gatctcggcg
     2761 tgcgccggca tcggcaggcc gtgcagctcg ctctcgagca cctcgatcgg cgcgccgcga
     2821 tggccgcccg cgtagtcgta ctccgacaga ccgaagcgca gctcgttgcc gccggcgagg
     2881 aagagcaggg gatcgtggcc gcacgagacg agcaccgggc agggcttgcc ctgcctgaag
     2941 tatttttcgc ggatctggcg gccgtgcttg cccggcgaca tccacacgcc gacgcgcttc
     3001 gcgtcgtgcg ccatgacgcg gtaggtgccg caattcaccc agccgccgtc ggggtcgcgc
     3061 atgatcacca ggtcgtccgt gccgatgtat cgaccgccgt cctgctcgtg gaggaaaggc
     3121 acggggaact tgagcacgtt caccgcgtcg tcgcgctgga cgttctcgag gaccgatccc
     3181 ttcttcacga cctcgggccg gatcgggcgg tgcgtctgca tgcggtcgcg gtacgcgcgc
     3241 acgacgtcga gcgggtgcga aggcgcggga aagccgagcg tcacggcgag ccgcttcatc
     3301 gagttggtcg cgcccgagag cacgcgcatg ccggcggggt agtcggggat ctcgtcgaac
     3361 agcaccgcgc gcgagcccgc cgggtgctgg ttgacgatct cggcgagcgt tcccatctcg
     3421 aggttccagt gcgcgcgcgg gatgcgcgcc agctcgccgg cgcgctcgac gcgcgccagg
     3481 aactcgcgca ggtcggcgtg cgggcggtcg tagtcgttgt acgcgccggg cttggccatg
     3541 tcgttcatcg ggcggctctc ttcgctggct ctcgccggga ttgtacttcc tcgggcgccg
     3601 gcggcaggtc ttcgcgatgc agcaggaaca ccaagccgtc gcccgcgctg gtttcgagcc
     3661 aggtgaatgg tgtcttcggg aacgcgcgct ccagctcgcg ccgatgccgg ccgatctcga
     3721 cgacgagcag cccgcgcggc cgcaggaagc ggcgtgcctc gtggaggatc cgccgcacga
     3781 aggcgagccc gtcgccgcct cccgccagcg ccaggcgcgg ctcgcggcgg tattcggcgg
     3841 ggacgctccg catcgcgcgc gcgtcgacgt acggaggatt ggccacgatg acgtcgtacg
     3901 gcccggcgcg aaaatcgcga aacagatccg agcgcaccag ccgcacgcgc gacttcaggc
     3961 gatacgccgc gacgttgcgc cgggcgacgg c
//
LOCUS       DATDMW010000025        10311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1374120, whole genome shotgun sequence.
ACCESSION   DATDMW010000025 DATDMW010000000
VERSION     DATDMW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374120"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VI319_00790"
     CDS             <1..501
                     /locus_tag="VI319_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862406.1"
                     /translation="QFGLQKKMAIGGGLSELEVMAALPPEARSGWWTVEWYWNQPKTP
                     HVKEFVSAYQKAYGKTPSARSWFGYAGMHSLVLGANKAKSIEPVKMAHALEGMVLPPE
                     VALQPHKIYFRPGDHQLMSSEFPGEIKPTGKYPDLFQVADIVEGDKIALPAAETHPGK
                     LDYPTA"
     gene            574..1443
                     /locus_tag="VI319_00795"
     CDS             574..1443
                     /locus_tag="VI319_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6862407.1"
                     /translation="MQLLLFNVFNGLVIGMFYALMALGLSLILSLNFTINMAHGGFLI
                     VGAYLAFTLNPYLGFWGALVAGPLCAAVLGLVVERLLIKPLYKRNEPLYSLLVTFGLA
                     LVIQDVARRIWGPQGLPFSIPEALNSPISEALFFITGYRALIVAIALIAAIALFLILQ
                     YTRLGVRIRAGTRDLETVAALGVNIYVLRALNFGLGCLFAGLAGVLAAGQLGLIPTMG
                     ESVIMPSFVAIIVGGVGSLVGSILGGLIIGVAAGVMTTYYPAASEAVIYLIMAVVLVV
                     RPRGLLGQEGLFE"
     gene            1440..2381
                     /locus_tag="VI319_00800"
     CDS             1440..2381
                     /locus_tag="VI319_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6862408.1"
                     /translation="MNRKIAALAVLWALLATAPWWLPYVGGYTQLGSRVLIYALATMS
                     LNLLLGFTGGLSFGHAAYFGLGGYGAGMTLKYLAPSTPLALLVGTLVGGAAATLLGPS
                     VMRRRGIYFAMITIAIGQLFYFVAVRWNEVTGGEDGLAGYSRQPLHLGGWTLPLDDTA
                     FYYLVLVCFAASVGIIGLLLRSPLGRTWVAIRENQRRAQFLGLTVPLYVWASFAISGT
                     LTALAGALNALLNNFTSPQDLHWILSGNFVIMVVLGGMRSFWGPLAGVIIFLVAQDYL
                     SSLTENWMTFIGIIFILVVLLFPRGLLGMLRSRLQPK"
     gene            2392..3141
                     /locus_tag="VI319_00805"
     CDS             2392..3141
                     /locus_tag="VI319_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862409.1"
                     /translation="MSLLRLESISRHFGSLVALQDVSLEMAAGELRAVIGPNGAGKTT
                     LFNMVSGFFPPSSGRVFFEGQPINADRAEARVRRGIVRTFQITEIFHELNVYENVCVA
                     VETAAGLNSRPWVSRETATRVRRRIDELLALTGLAAKADRVASELAHGDQRVVEVAIA
                     LAMRPRLLLLDEPTAGMGDEETDRMVGLIRRLHQEQHLSILFIEHDMDIVFGIAQRIT
                     VLDQGRILAEGSPAEVSANEKVRAAYLGEAA"
     gene            3138..3845
                     /locus_tag="VI319_00810"
     CDS             3138..3845
                     /locus_tag="VI319_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862410.1"
                     /translation="MSGAVLEVSGIHTYYGKSHILNGVDLEVREGEIVALLGRNGAGK
                     TTTLRSLVGLTPPRSGRIRIFGRDTAGWPAHKIARLGVGYVPEGRKIFGALTVAENLL
                     VPQSAPGPWTEERVLKLFPRLAERRAQLGRQLSGGEQEMLSIARPLLLNPKLMLLDEP
                     SQGLAPLIVREVMQVVRTMRDEGLSVLLVEQNVPLSLGIADRAYILSDGRVVYSGAAD
                     ELARNTELVHKLAGAGN"
     gene            4094..5236
                     /locus_tag="VI319_00815"
     CDS             4094..5236
                     /locus_tag="VI319_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEY6862411.1"
                     /translation="MSKEQVKKPARRKFLAGAAAATAAGATLGFPMIAKGQTGPINFR
                     FQSTWPSKDIFHEYAQDYAKIVNDMTGGDLKIDVLPAGAVVPAFGLLEAVSKGTLDGG
                     HGVLVYSYGKSNSLALWGSGPGFGMDANMLLAWHHHGGGKELLEKLYASIGGNVVSFP
                     YAPLYTQPLGWFKKPMTKPSDFKGLKYRTVGISIDVFTAMGAAVNALPGGEIVPAIDR
                     GLLDAAEFNNTSSDRALGFADVSKVCMLQSYHQNAEQLEITFNKTKYDALPAKLKAII
                     SNAVDAASAQMMWKAIDRNSQDYITLQTVDKVRFYKTPASILRAQLDAYDQAAAKKMS
                     TPFFKEIIESQKKFAQRAVKWEQDVVVDRKMAYDHYFGKQAASKKA"
     gene            5321..6031
                     /locus_tag="VI319_00820"
     CDS             5321..6031
                     /locus_tag="VI319_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY6862412.1"
                     /translation="MNAQKVERLLQIADHVSTWAGKSSAWLILLLMSTVCIEVFKRYI
                     LNMPTAWIFDATNMMYGTLFMLCGAYTLAQDAHVRGDFLYSSMRPRTQAVLDLVLYVV
                     FFVPGVIALIYAGTDYAAYSWSIDEHSNVTSNGPAVYPFKTVIPIAGVLILIQGAAEI
                     ARCVVCLKTGDWPARLADAEEIDVVEQQLANSAYVDEESRKRAVESVHEIDEVAHQRG
                     LGERATEEVARHPQGERK"
     gene            6028..7899
                     /locus_tag="VI319_00825"
     CDS             6028..7899
                     /locus_tag="VI319_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY6862413.1"
                     /translation="MSDPALGLTMLALIVIVIMLGFPTAFTLMGLGMFFGYIALYDPS
                     HSWLDNRVFDLMVERTYGAMNNDTLISIPLFVLMGYVMERGALVDKMFYSIQLAFRRV
                     PASLAVATLIVCTFWGIASGLVGAVVVLMGVIAFNPMLKAGYNTKLASGVITAGGTLG
                     ILIPPSVMIIVYAAVAGQSVVKLYASAMFPGFFLAFLYLVYIVGWAMIDPKIAPKLPE
                     SETKVPVSDWVRSFQKAYSNNLLLGLLAAVVNPGRALGIAVGEGRRVTYVMLWKNLLA
                     ALFPLMLSAVTLWGVWWYVVIHQQADTQATAGMVIRQQDTAQAAPQANPDKPQELGNA
                     EADSDTSAQEAEGVEQVGTPELLQQGAAAPAESGPPQGFYLYFWLVVAATALVLLWYY
                     WQLGAEPFELLKLLISSVMPLGILTIVVLAVILFGITTATESAAIGAAGAFILALQAG
                     TFSWERMKQSVFLTAKTTAMVCWLFIGSALFSGVFAILGGQQLVETWVLSLNMSPIQF
                     MILSQAIIFVLGWPLEWTEIIVIFVPIFLPLLKHFNIDPILWGTLVFVNLQAAFLSPP
                     VAMSAFYLKGVAPKHVTLNQIFAGMMPYMIIVIVCMVIMYLWPGMTLWLPNYLYGGG"
     gene            7886..8887
                     /locus_tag="VI319_00830"
     CDS             7886..8887
                     /locus_tag="VI319_00830"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY6862414.1"
                     /translation="MAAGERKKILVCRQTFDEVIESLAERFDVEHNQATDTPWSADEL
                     RRRLADKDGVLTSGADRIDAAVVADAPRLRAVCNVAVGYNNLDVAALAERGILATNTP
                     AVLDDTTADTAFALMLAAARRLPESERWLRAGHWKGWKNDQFLGVDVHHATLGIVGMG
                     RIGQAIAQRARGFGMKVVYYNRRRCPAAVEKAAGAKYVPLEKLLKNSDFVSLNLPYSA
                     EAHHLIGAKQLALMKPTAVLVNAARGGVVDDAALIRALKEKRIFAAGIDVFEGEPRFN
                     PGFLELDNVALVPHIGSATRATRMKMNLLAVKNLVAALSGKRPPNLVNPDAWPKRRR"
     gene            8953..9396
                     /locus_tag="VI319_00835"
     CDS             8953..9396
                     /locus_tag="VI319_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6862415.1"
                     /translation="MEKPLVKKTFPTPQDAETAFYEALENADLDAMMEVWAEDEEIVC
                     VHPGGPRLAGYDQVRAGWAQIFGSGQRLKVFLNNPVVLSGMMLSIHSVHENITVAGEG
                     RPRPPVAATNVYLRTGNGWRLLLHHASPVPQAQQQRGGDSPKILH"
     gene            9410..>10311
                     /locus_tag="VI319_00840"
     CDS             9410..>10311
                     /locus_tag="VI319_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6862416.1"
                     /translation="MRAAYRAPGWLPGGNAQTLYAFALGRRSRAAFARERWRTPDGDF
                     IDVDRSAHRAPPGSPRVVLFHGLEGGSRSHYAGAFARELARLGWRLAVPHFRGCSGEP
                     NLLPRAYHSGDWAEIQWILRRFRDEDPGATLYAVGVSLGGNALLRWLAEDERAADTVA
                     AAAAVSAPMDLMAAGDALGRGFNLVYARAFLATMKPKALAMLERHPGLFDAAAVRASR
                     TLRAFDDCVTAPLHGFRDVDDYWTRASAKPGLGRIRVPTLIVNARNDPFLPASALPDP
                     AALPAAVRCEFTEEGGHVGYVTGP"
BASE COUNT         1572 a         3589 c         3469 g         1681 t
ORIGIN      
        1 cagttcggcc tgcagaagaa gatggcgatc ggcggcggcc tgtcggagct cgaggtgatg
       61 gcggcgctgc cgcccgaggc gcgctcgggc tggtggacgg tggagtggta ctggaaccag
      121 ccgaagaccc cgcacgtgaa ggagttcgtc tccgcctacc agaaggccta cggcaagacc
      181 ccgagcgcgc ggagctggtt cggctacgcc ggcatgcatt cgctcgtgct cggcgcgaac
      241 aaggcgaaga gcatcgagcc ggtgaagatg gcgcacgcgc tcgagggcat ggtgctgccg
      301 cccgaggtcg cgttgcagcc gcacaagatc tacttccgcc cgggcgacca ccagctgatg
      361 tcgagcgagt tccccggcga gatcaagccc accggcaagt accccgatct gttccaggtc
      421 gcggacatcg tcgagggcga caagatcgcg ctcccggccg cggagacgca ccccggcaag
      481 ctcgactacc cgacggcctg atcgaacggc cgctctcaca ggcgcggcgc gcgggactcc
      541 cgcacgccgc gcttttcata tagcgaacga cgcatgcagc tgctgctctt caacgtcttc
      601 aacggcctcg tgatcgggat gttctacgcc ctgatggcgc tcggcctgtc gctgatcctc
      661 agcctgaatt tcacgatcaa catggcgcac ggcgggttcc tgatcgtcgg cgcctacctc
      721 gcgttcacgc tgaatcccta cctcgggttc tggggcgcgc tggtcgcggg gccgctgtgc
      781 gccgccgtgc tcgggctggt ggtcgagcgg ctgctcatca agccgctgta caagcgcaac
      841 gagccgctgt actcgctgct cgtcaccttc ggcctcgcgc tcgtcatcca ggacgtcgcg
      901 cgccgcatct ggggaccgca ggggctgccg ttctcgatcc ccgaggcgct caactcgccg
      961 atcagcgagg cgctgttctt catcaccggc taccgggcgc tgatcgtcgc catcgcgctg
     1021 atcgcggcca tcgcgctgtt cctgatcctg cagtacaccc ggctcggcgt gcgcatccgc
     1081 gccggcacgc gcgacctcga gaccgtggcc gcgctcggcg tcaacatcta cgtgctgcgc
     1141 gcgctcaact tcggcctggg ctgcctgttc gccgggctcg cgggcgtgct cgccgccggc
     1201 cagctcggcc tgatcccgac catgggcgag agcgtgatca tgccgagctt cgtcgcgatc
     1261 atcgtcggcg gcgtgggaag cctggtcggc agcatcctcg gcggcctgat catcggcgtc
     1321 gccgcgggcg tcatgaccac gtactacccg gcggcgagcg aggcggtcat ctacctgatc
     1381 atggcggtgg tgctggtcgt gcggccgcgc ggcctgctcg ggcaggaggg cctgttcgaa
     1441 tgaaccgcaa gatcgcggcc ctcgcggtgc tctgggcgct gctcgcgacc gcgccgtggt
     1501 ggctgccgta cgtcggcggc tatacgcagc tcggctcgcg cgtgctgatc tatgcgctcg
     1561 cgacgatgtc gctgaacctg ctgctcgggt tcaccggcgg cctctcgttc gggcacgcgg
     1621 cgtatttcgg gctgggcggc tacggcgcgg gcatgacgct caagtacctc gcgccgagca
     1681 ccccgctcgc cctcctcgtc gggacgctgg tcggcggcgc cgcggcgacg ctgctcgggc
     1741 cctcggtgat gcgccggcgc ggcatctatt tcgcgatgat cacgatcgcg atcgggcagc
     1801 tgttctattt cgtcgccgtg cgctggaacg aggtgaccgg cggcgaggac gggctggccg
     1861 gatactcccg ccagccgctg cacctcggcg gctggacgct gccgctcgac gacaccgcgt
     1921 tctactacct cgtgctcgtg tgtttcgccg ccagcgtggg gatcatcggc ctgctgctgc
     1981 gctcgcccct cgggcgcacc tgggtcgcga tccgcgagaa ccagcggcgc gcgcagttcc
     2041 tcggactgac cgtcccgctc tacgtctggg cctcgttcgc gatctcgggc acgctgaccg
     2101 cgctcgcggg cgcgctgaac gcgctgctca acaacttcac ctcgccgcag gacctgcact
     2161 ggatcctgtc gggcaacttc gtgatcatgg tggtgctcgg cggcatgcgc agcttctggg
     2221 gtccgctcgc gggcgtcatc atcttcctcg tcgcgcagga ctacctcagc agcctgaccg
     2281 agaactggat gaccttcatc ggcatcatct tcatcctggt cgtgctgctg ttcccgcgcg
     2341 gcctgctcgg catgctgcgc agccggctcc agcccaaatg agaggcgcgc gatgagcctg
     2401 ctgcgcctcg agagcatctc gcgccacttc ggcagcctgg tcgcgctcca ggacgtctcg
     2461 ctcgagatgg ccgcgggcga gctgcgcgcg gtgatcgggc cgaacggcgc cggcaagacg
     2521 acgctgttca acatggtgag cggcttcttc ccgccctcga gcgggcgcgt gttcttcgag
     2581 ggccagccga tcaacgccga ccgcgccgag gcgcgcgtgc gacggggcat cgtgcgcacc
     2641 ttccagatca ccgagatctt ccacgagctg aacgtgtacg agaacgtgtg cgtggcggtc
     2701 gagaccgcgg ccggcctcaa ttcccgcccc tgggtctcgc gcgagaccgc cacgcgcgtg
     2761 cgccggcgca tcgacgagct gctcgcgctc accgggctcg cggcgaaggc cgaccgtgtc
     2821 gcgagcgagc tcgcccacgg cgaccagcgc gtcgtcgagg tcgcgatcgc gctcgcgatg
     2881 cggcccaggc tgctgctcct cgacgagccg accgccggca tgggcgacga ggagaccgac
     2941 cgcatggtcg gcctgatccg ccggctgcac caggagcagc atctgagcat cctgttcatc
     3001 gagcacgaca tggacatcgt gttcggcatc gcgcagcgca tcacggtgct cgaccaggga
     3061 cggatcctcg ccgagggctc gcccgccgag gtctcggcga acgagaaggt gcgcgccgct
     3121 tatctcgggg aggcggcatg agcggcgcgg tgctcgaggt ctcggggatc cacacctact
     3181 acggcaagag ccacatcctg aacggcgtcg acctcgaggt gcgcgaaggc gagatcgtgg
     3241 ccctgctcgg ccgcaacggc gccggcaaga ccacgacgct gcgcagcctc gtcggcctca
     3301 cccccccgcg cagcggccgg atccggatct tcggccgcga caccgccgga tggcccgcgc
     3361 acaagatcgc gcggctcggg gtcgggtacg tgcccgaggg ccgcaagatc ttcggcgccc
     3421 tgaccgtcgc cgagaacctg ctcgtgccgc agtcggcgcc cgggccctgg accgaggagc
     3481 gggtgctgaa gctgtttccg cggctggccg agcgcagggc gcagctcggg cgccagctct
     3541 cgggcggcga gcaggagatg ctctcgatcg cgcggccgct cctcctcaat ccgaagctga
     3601 tgctgctgga cgagccctcg cagggactcg cgccgctcat cgtgcgcgag gtgatgcagg
     3661 tcgtgcgcac catgcgcgac gaggggctgt cggtgctgct ggtcgagcag aacgtgccgc
     3721 tgtcgctcgg catcgccgac cgcgcctaca tcctgagcga cggccgggtc gtgtactcgg
     3781 gcgccgcgga cgagctcgcg cggaacaccg agctcgtgca caagctcgcc ggcgccggca
     3841 actgaggccg cgctcggggc gccgccgcca aagctcccgc ccgaccacgg ggcgacccgg
     3901 cgtccgccgg gcgccttcgc ttgatttcac ccggacttcg gaataggatt cgcgggcttt
     3961 ggcgtccgac gagaagaata cgtgggccac ggcgggaaca gcgagggaac gtggctcgta
     4021 acccgggggt tcccggagaa caagaagaac gccgaaacgc ttatccaacc gccattccaa
     4081 gtaggaggat ccaatgagca aggagcaagt caagaagccg gcgcgtcgca agttccttgc
     4141 gggcgcggct gccgcgaccg cggccggggc gacgctggga ttcccgatga tcgccaaggg
     4201 ccagaccggc ccgatcaatt tccgattcca gtcgacgtgg ccctcgaagg acatcttcca
     4261 cgagtacgcg caggactacg cgaagatcgt caacgacatg accggcggcg atctcaagat
     4321 cgacgtgctg cccgccgggg cggtcgtgcc ggccttcggc ctgctcgagg cggtgtccaa
     4381 gggcacgctc gacggcggcc acggcgtgct cgtctattcc tacggcaaga gcaactcgct
     4441 cgccctgtgg ggctcgggcc cgggcttcgg catggacgcc aacatgctgc tcgcctggca
     4501 tcaccacggc ggcggcaagg agctgctcga gaagctctac gcgtcgatcg gcggcaacgt
     4561 cgtttcgttc ccgtacgcgc cgctctacac gcagcccctg ggctggttca agaagccgat
     4621 gaccaagccg tccgacttca agggcctcaa gtaccgcacc gtcggcatct cgatcgacgt
     4681 gttcaccgcg atgggcgcgg cggtcaacgc gctgcccggc ggcgagatcg tgcccgccat
     4741 cgaccgcggc ctgctcgacg cggccgagtt caacaacacc tcgtcggacc gcgcgctcgg
     4801 cttcgccgac gtctcgaagg tctgcatgct gcagagctac caccagaacg cggagcagct
     4861 cgagatcacc ttcaacaaga ccaagtacga cgcgctgccg gccaagctga aggcgatcat
     4921 ctccaacgcc gtcgacgccg cctcggcgca gatgatgtgg aaggcgatcg accgcaactc
     4981 gcaggactac atcacgctgc agacggtgga caaggtccgg ttctacaaga cccccgcctc
     5041 gatcctgcgc gcgcagctcg acgcctacga ccaggcggcg gcgaagaaga tgagcacgcc
     5101 gttcttcaag gagatcatcg agtcgcagaa gaagttcgcg cagcgcgcgg tgaagtggga
     5161 gcaggacgtg gtcgtcgacc ggaaaatggc gtacgaccat tacttcggga agcaagcggc
     5221 ctcgaagaag gcctgaggca acgccgacga gggcaccatc cgggaccgtc ccggctggtg
     5281 cctcgttttt tgtaggacgc aacagggagc ttcgccagtc atgaacgctc agaaggtcga
     5341 gcgcctgctc cagatcgcgg accacgtcag cacatgggcc ggaaaatctt ccgcctggct
     5401 gatcttgctg ctgatgtcca cggtctgcat cgaggtgttc aagcgctaca tcctcaatat
     5461 gccgacggcg tggatattcg acgccaccaa catgatgtac ggcacgctgt tcatgctgtg
     5521 cggggcctat acgctggcgc aggacgctca cgtgcgcggc gattttctgt acagctcgat
     5581 gcggccgcgc acgcaggccg tcctcgacct ggttctctac gtcgtgttct tcgtccccgg
     5641 cgtcatcgcg ctgatctacg cgggcacgga ttacgccgcc tactcctgga gcatcgacga
     5701 gcactcgaac gtcacctcca acggccccgc cgtctatccg ttcaagacgg tgatccccat
     5761 tgccggggtg ctgatcctga tccagggcgc ggctgagatc gcgcgctgcg tggtctgcct
     5821 caagacgggc gactggccgg cgcgactcgc cgacgcggag gagatcgacg tggtcgagca
     5881 gcagctcgcg aacagcgcgt acgtggacga ggagtcgcgc aagcgcgcgg tcgagagcgt
     5941 gcacgagatc gacgaggtcg cgcaccagcg cggcctcggc gagcgtgcga ccgaagaggt
     6001 cgcccggcat ccccagggag agcgcaagtg agcgatcccg cactcgggct gacgatgctc
     6061 gcgctcatcg tgatcgtgat catgctgggc tttccgaccg cgttcacgct gatgggcctg
     6121 ggcatgttct tcggctacat cgcgctctac gacccttcgc actcctggct ggacaaccgg
     6181 gtcttcgacc tcatggtcga gcgcacttac ggcgcgatga acaacgacac gctgatatcg
     6241 atcccgctgt tcgtgctcat gggctacgtg atggagcgcg gcgcgctggt cgacaagatg
     6301 ttctacagca tccagctcgc gttccggcgc gtccccgcct cgctcgcggt tgccacgctg
     6361 atcgtgtgca ccttctgggg aatcgcgagc ggcctggtcg gcgcggtcgt ggtcctgatg
     6421 ggcgtgatcg cgttcaaccc gatgttgaag gccgggtaca acacgaagct cgcctcgggc
     6481 gtcatcacgg ccggcggcac gctcggcatt ctgatcccgc cctcggtcat gatcatcgtc
     6541 tacgccgcgg tggccggcca gtcggtcgtg aagctctacg cctcggcgat gttccccggg
     6601 tttttcctgg ccttcctgta cctcgtgtac atcgtgggct gggcgatgat cgaccccaag
     6661 atcgctccca agctgcccga gtcggagacc aaggtgccgg tgtcggactg ggtgcggagc
     6721 ttccagaagg catactcgaa caacctgctg ctcgggctgc tcgccgcggt cgtcaatccc
     6781 ggcagggcgc tcggcatcgc cgtcggcgag ggccgccgcg tcacctacgt catgctctgg
     6841 aagaacctgc tcgccgcgct cttcccgctg atgctgtccg ccgtgacctt gtggggcgtc
     6901 tggtggtacg tggtcattca ccagcaggcc gatacccagg ccacggccgg catggtgatc
     6961 cggcaacagg acaccgctca ggccgcgccg caggcgaacc cggacaagcc gcaggagctc
     7021 gggaacgccg aggccgattc cgacacgtcg gcgcaggaag ccgaaggcgt ggagcaggtg
     7081 ggcacccccg agctcctgca gcaaggcgcg gccgctccgg ccgaatccgg tcctccgcag
     7141 ggcttctacc tgtatttctg gctcgtcgtc gccgccaccg ccctggtgct gctctggtac
     7201 tactggcagc tcggggccga gccgttcgag ctgctgaagc tgctgatctc gtcggtgatg
     7261 ccgctcggca tcctcaccat cgtggtgctt gccgtcatcc tgttcggcat caccacggcg
     7321 acggagtctg cggctatcgg ggcggccggg gcgttcatcc tggcgctcca ggcgggaacg
     7381 ttcagctggg agcgcatgaa gcagtcggtt ttcctgaccg cgaaaaccac ggccatggtg
     7441 tgctggctgt tcatcggctc ggcgctgttc tccggcgtgt tcgcgattct cggcgggcag
     7501 cagctggtcg agacctgggt gctgtcgctc aacatgtcgc cgatccagtt catgatcctg
     7561 tcgcaagcca tcatcttcgt cctgggatgg ccgctggaat ggaccgagat catcgtcatc
     7621 ttcgtgccga tcttcctgcc gctgctgaag cacttcaaca tcgacccgat cctgtggggg
     7681 acgctggtgt tcgtcaacct gcaggcggcg ttcctctcgc cgccggtggc gatgtcggcg
     7741 ttctacctga agggcgtcgc gcccaagcac gtgacgctga accagatctt cgcgggcatg
     7801 atgccgtaca tgatcatcgt catcgtctgc atggtgatca tgtacctgtg gccggggatg
     7861 acgctgtggc tgccgaacta tctctatggc ggcgggtgag cggaagaaga tcctcgtctg
     7921 ccggcagacg ttcgacgagg tgatcgagtc gctcgccgag cgcttcgacg tcgagcacaa
     7981 ccaggcgacc gacacgccct ggagcgccga cgagctgcgc cggcgcctcg ccgacaagga
     8041 cggcgtgctc acctcgggcg ccgaccgcat cgatgccgcc gtcgtcgcgg acgcgccgcg
     8101 cctcagggcg gtctgtaacg tcgccgtcgg ctacaacaac ctcgacgtcg ccgcgctcgc
     8161 cgagcgcggc atcctcgcga ccaacacgcc ggccgtgctc gacgacacga ccgccgacac
     8221 ggcgttcgcg ctgatgctcg cggcggcgcg gcgcctgccc gagtccgagc gctggctgcg
     8281 cgcagggcac tggaagggct ggaagaacga ccagttcctc ggcgtcgatg tgcaccatgc
     8341 gacgctcggc atcgtcggca tggggcgcat cggccaggcc atcgcgcagc gcgcgcgcgg
     8401 cttcgggatg aaagtcgtgt actacaaccg caggcgctgc cccgcggcgg tcgagaaggc
     8461 ggcgggcgcg aagtacgtgc cgctcgagaa gctcctgaag aactccgact tcgtcagcct
     8521 gaacctgccg tactcggccg aggcgcatca cctgatcggc gcaaagcagc tcgcgctcat
     8581 gaagcccacc gcggtgctgg tgaacgccgc ccgaggcggc gtcgtcgacg acgccgcgct
     8641 catccgggcg ctgaaggaga agcgcatctt cgccgccggc atcgacgtgt tcgaaggcga
     8701 gccgcggttc aatcccggct tcctcgagct cgacaacgtc gcgctcgtgc cgcacatcgg
     8761 cagcgcgacg cgcgccacgc gcatgaagat gaacctgctc gcggtgaaga acctggtcgc
     8821 cgcgctctcg ggcaagcgcc cgcccaatct cgtcaatccc gacgcctggc cgaagcggcg
     8881 ccgctgagcg ccgcccgccg gctggcggcg cccggccgtt tccgataccc tacgcggctc
     8941 cggggccgca tgatggaaaa gccgctcgtg aagaagacct tcccgacgcc tcaggacgcc
     9001 gagactgcgt tctacgaggc gctcgagaac gccgatctcg acgcgatgat ggaggtctgg
     9061 gccgaggacg aggagatcgt ctgcgtccac ccgggcggac cgcggctcgc aggctacgac
     9121 caggtgcgcg ccggctgggc gcagatcttc ggctcgggac agcggctcaa ggtgttcctc
     9181 aacaacccgg tggtcctctc gggcatgatg ctctcgatcc acagcgtgca cgagaacatc
     9241 accgtggcgg gcgagggccg cccgcggccg ccggtcgccg cgaccaacgt ctacctgcgc
     9301 accggcaacg gctggcggct gctcctgcac cacgcgtcgc ccgtgccgca ggcgcagcag
     9361 cagcgcggcg gcgattctcc gaagatcctg cactagcgtc cgaaccgaca tgcgggccgc
     9421 gtaccgcgcc cccgggtggc tgccgggcgg caacgcccag acgctgtacg ccttcgcgct
     9481 cggccggcgc tcgcgcgccg ccttcgcgcg cgagcgctgg cgcacgcccg acggcgactt
     9541 catcgacgtc gaccgcagcg cgcatcgtgc gccgccggga tcgccgcgcg tcgtgctgtt
     9601 ccatggcctc gagggcggct cgcgcagcca ttacgcgggg gcgttcgcgc gcgagcttgc
     9661 acgcctgggc tggcgcctcg cggtgccgca cttccgcggc tgctcgggcg agccgaacct
     9721 gctgccgcgc gcgtaccact cgggcgactg ggcggagatc cagtggatcc tgcggcgctt
     9781 ccgcgacgag gacccgggcg ccacgctcta cgccgtcggc gtctcgctcg gcggcaacgc
     9841 gctgctgcgc tggctcgcgg aggacgagcg cgccgcggac accgtcgccg ccgccgcggc
     9901 ggtgtccgcg ccgatggatc tgatggcggc cggcgacgcg ctcggccgcg gcttcaacct
     9961 ggtctatgcg cgcgccttcc tcgcgaccat gaagccgaag gcgctcgcga tgctcgagcg
    10021 ccaccccgga ctcttcgacg ccgcggccgt gcgcgcctcg cgcacgctgc gcgcgttcga
    10081 cgactgcgtc accgcgccgc tgcacggctt ccgcgacgtc gacgactact ggacgcgcgc
    10141 gagcgccaag cccggcctcg gacgcatccg cgtgccgacg ctgatcgtga acgcgcgcaa
    10201 cgacccgttc ctgccggcga gcgcgctgcc cgaccccgcg gcgcttccgg ccgcggtgcg
    10261 ctgcgagttc accgaggagg gcggtcacgt cggctacgtc accgggccgt t
//
LOCUS       DATDMW010000026         2424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_752113, whole genome shotgun sequence.
ACCESSION   DATDMW010000026 DATDMW010000000
VERSION     DATDMW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752113"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="VI319_00845"
     CDS             complement(<1..1850)
                     /locus_tag="VI319_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862417.1"
                     /translation="MRPVLSPAEALGLSGATLEARIRRAANHVTDAAFARIDERLRAD
                     ARTNRLIYEHEGVEEPIRLMLRPLLVMQEQLSYVHHVCLQLIEALKRVPDLYLGDERI
                     RTIVAITPDEERWFRNTWTEAHQRFNSIYGRLDAVCDFTGAGWQDSLHFMEPNLSGVG
                     GIHFAPVAEQLVMRDIVPTLLGHDPGLVIEMPRDQRDLFIQVLIDHARTIGRESCQLC
                     FVEAKYVHDGPNEQSVLSDYLSHRHNLTVTHADPRELRVEGDEVFYENVRIDVAYRDY
                     EMRELVALEKEIGRPLDAMRLLFRQNRVVSSIVGDFDHKSCFEILTDPMIAEQYFGAD
                     DRRLFRRHVLWTRVVADRRTTLPDDREGDLLEYARRNRELLVLKPNRAYGGTGVMLGA
                     ATEEGEWEQALQAAVLQARDPEQSWVVQSATRLPVHEFPVVGPDGRVFGEPFYAVMGF
                     AATENGLGTMCRVSQKQVVNVAQRGGLAAVLEAEAPAELRIPKRSVGRSEAMAQSLRA
                     QISELRHLDQTIALLDWDEETMLPLAARAGRGEQLATLEGLRHALLVSDRLGDLVEEV
                     AAQSEGNERLARELGLLRRLRLRALAMPEDLVRQFANAKSQSLGAWEEAR"
     gene            complement(1948..>2424)
                     /locus_tag="VI319_00850"
     CDS             complement(1948..>2424)
                     /locus_tag="VI319_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY6862418.1"
                     /translation="DTTRNQLGLAMSMFIEHPDQWRLLAERPELARNAVEEVMRVRPT
                     VTWVTREATEDFVYRDLELRRGTTIHLFSESAGTDPRVFAPGFDIAAERKPHFGFGGG
                     VHHCLGHFIARADMTEALKVLVRRIKNPRYDGEPAWLPDSGNTGAVKLPIAFEAPS"
BASE COUNT          453 a          779 c          763 g          429 t
ORIGIN      
        1 ttgcgcgcct cctcccaagc gcccagcgat tgcgatttgg cattggcgaa ctgccgcacc
       61 aaatcctcgg gcattgccag cgcacgcagc cgcagccggc gaagcagccc gagctcgcgc
      121 gcgaggcgct cgtttccctc cgactgagcg gcaacctctt cgacgagatc gccaagccgg
      181 tcggaaacca gcagcgcgtg gcgaagacct tcgagcgtgg cgagctgctc tccgcgcccg
      241 gcgcgcgcgg ccagcggcag catcgtttcc tcatcccagt cgagcagcgc aatggtctgg
      301 tcgaggtgac gcaattcgga gatctgggcg cgcagcgact gcgccatcgc ctcggagcgg
      361 ccgacggacc gcttggggat ccgcaactcg gccggcgctt ccgcctctag gactgcggca
      421 agtccaccgc gctgcgcgac attgacgacc tgcttttgcg agacgcggca catggtgccg
      481 aggccattct cggtcgcggc aaatcccatc actgcataaa agggctcgcc gaacacgcgc
      541 ccgtcggggc cgacgaccgg aaactcatgc accggaagac gcgtcgcgct ctgcacgacc
      601 cacgactgct cgggatcgcg ggcttgcagc accgctgctt gcaaagcctg ttcccactct
      661 ccctcttcgg tcgcagcgcc caacatgacg ccggtgccgc cataggcgcg gtttggtttc
      721 aggaccaaga gctcgcggtt tcgccgcgca tattccagca ggtccccttc cctgtcgtca
      781 ggaagtgtcg tgcggcgatc agcgaccacg cgcgtccaca agacatgacg acggaacagc
      841 ctgcggtcgt cggccccgaa atattgctcg gcgatcatcg ggtcggtcag gatctcgaag
      901 cagctcttgt gatcgaaatc gccgacgatc gaggagacga cgcggttttg ccggaacaga
      961 agccgcatgg cgtcgagcgg gcggcctatc tccttttcga gcgccactag ctcgcgcatc
     1021 tcgtagtcgc gataagcgac gtcgatccga acgttctcgt agaagacctc gtcgccctcg
     1081 acgcgcagtt cgcgcgggtc ggcatgagta acggtcagat tgtgccggtg agacagataa
     1141 tcgctcagca ccgactgctc gttgggaccg tcatggacgt atttcgcctc gacgaaacag
     1201 agctggcatg actcgcgccc aatggtgcgc gcatggtcga tcaacacctg aatgaataga
     1261 tcgcgctgat ccctcggcat ctcgatgacg agtccagggt cgtgcccaag cagagtcggc
     1321 acgatgtcgc gcatcacaag ctgctcggca accggcgcga agtggatgcc gcccacgccg
     1381 gaaaggttcg gctccatgaa atggagcgaa tcctgccaac cggcgcccgt gaaatcgcat
     1441 accgcatcga gccggccata gatcgagttg aagcgctggt gggcttcggt ccaggtattg
     1501 cggaaccagc gctcctcgtc gggcgtgatg gcaacgatag tcctgatccg ctcgtctccg
     1561 agatagaggt caggcacgcg cttgagggct tcgatgagct gaagacagac gtggtggacg
     1621 tagctcagct gctcctgcat gacgagcagc gggcgcagca tgaggcgaat gggctcttcg
     1681 acgccttcat gctcgtagat cagtcggtta gtccgcgcgt cggcacgcaa gcgttcgtca
     1741 atccgtgcga aggccgcgtc ggtgacgtga ttcgccgcgc gccttatccg cgcttcgagc
     1801 gtcgcgccgg agagcccgag ggcttctgca ggactgagga ctggccgcat atctcgagac
     1861 accgggtgag gaccgtaccg ctaaagacgg gtcactctat gcgaagactt ataaaaatag
     1921 gtatcgcaga tgttgccttg accgtattca ggatggcgcc tcgaaggcga ttggtagctt
     1981 gactgcgccg gtattcccgc tgtccggcag ccaggccggc tcaccgtcgt agcgcgggtt
     2041 cttgatgcgc cggaccagca ccttcagcgc ctcggtcatg tcggcgcggg ctatgaaatg
     2101 gccgaggcaa tgatgcacgc cgccgccaaa gccgaaatgc ggcttgcgct ccgccgcgat
     2161 gtcgaagccc ggcgcgaaga cccggggatc ggtcccggca gattcggaga agaggtggat
     2221 cgtcgtgccc cgcctgagct ccagatcgcg atagacgaag tcctctgtcg cctcacgcgt
     2281 gacccaggtc accgtcgggc ggacccgcat cacctcctcg accgcgttcc tggcaagctc
     2341 aggccgctcg gcaaggagac gccattgatc aggatgctcg ataaacatgc tcatcgccag
     2401 gccgagctgg ttgcgcgtcg tgtc
//
LOCUS       DATDMW010000027         9589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_130350, whole genome shotgun sequence.
ACCESSION   DATDMW010000027 DATDMW010000000
VERSION     DATDMW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130350"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VI319_00855"
     CDS             <1..358
                     /locus_tag="VI319_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY6862419.1"
                     /translation="VLFDGERLGGFIDFYFAGVDAFLFDVAVTVNDWCIDSGGELDAE
                     RGRALLDAYAGVRAFTAEERRAWPAMLRAGALRFWLSRLYDFYLPRPGELTHAHDPEH
                     FRRVLESRRERALPLP"
     gene            368..1129
                     /locus_tag="VI319_00860"
     CDS             368..1129
                     /locus_tag="VI319_00860"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEY6862420.1"
                     /translation="MRIVPAKNGAAWLARGLVLFRRNPPAWLLAVLVYWLAVGLLEQL
                     PHVGRALSMLVLPPFTMSFMALCALLEAGAPLRPRPLVDAFRTRLPVLVTLGALYLAC
                     VLAVLALASLVDDGVLLDWALEGTEPPPDAIAEGRASRGLLAGALGLTPVMMAFWFAP
                     PLAGWGGMGAVQSLFYSFFAAWRNWRPFLVYGAVLMLGGVAVVAAMSMVAVLTHGNAD
                     SVHAALLVATLLTLPILFGSFYASYRDVFPEAGAA"
     gene            1167..1619
                     /locus_tag="VI319_00865"
     CDS             1167..1619
                     /locus_tag="VI319_00865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY6862421.1"
                     /translation="MPAREGDTTAAGNAAARCELCASDGGEVLWRDSFCRIVGVADAD
                     YPGFCRVVLQRHVKEMTDLEREERARLMDAVFATEAALRELLRPDKVNLASLGNITQH
                     LHWHVIPRFADDRHFPQPIWAQPARPRASRRAPGSSALADALRRRLGD"
     gene            1624..3210
                     /locus_tag="VI319_00870"
     CDS             1624..3210
                     /locus_tag="VI319_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862422.1"
                     /translation="MSSFDLPAAAPDANPDFRDAAGCAAWLQGVPLVNVASSHGKLIA
                     QLAELNRCAIAPAERLKILEQLRPAVLFVQAEHAKKFANRPVPLSPPEREAFNAVTSL
                     WGAFLVGYQYCLEAETDAAQAAVACQRALWCLSEKIGEHHATYQEIAPENWKLLNRVY
                     ALAEERGVAEREVDHPEYKKIETRCVETYARALLLNLANPNEHSPRQTAVIARWLERW
                     SQKVRISRTPPAGDQAPLAVDLAGDGGAARSGATGETVRYLDVSDIARSVKKRVSLLR
                     KGESPSALNLGDVPPATAEQLLIMLFKQWCEDKHSRVHPRRAASGTADLCIGMPAMHF
                     YVSGERFRQPAGLEELSKMQREEIATFGRVATRHEEEDYAKTQGFFLETWQLKDESRS
                     GLRLERPDRSAAARYQLHQLIAVRPADGKSFILGMVRWLSVAPDLLLRAGVRIMPGMP
                     KGVSVRGTGLNATNERFTAALLLPAAAALGSPESLVIPNGWFKPNRVIELIRESSSNV
                     VLTALLERGVDYERVTFEPA"
     gene            3234..3590
                     /locus_tag="VI319_00875"
     CDS             3234..3590
                     /locus_tag="VI319_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY6862423.1"
                     /translation="MGRVPTEIKLHSKSRIMEITFDDGERFELSYEFLRVYSPSAEVR
                     GHGPGQEVLQAGKKDVDIVALEPVGTYAVQPRFSDGHATGIYSWDYLHELGTNRDALW
                     QDYLERLRRSGARREP"
     gene            3596..4327
                     /gene="ubiE"
                     /locus_tag="VI319_00880"
     CDS             3596..4327
                     /gene="ubiE"
                     /locus_tag="VI319_00880"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY6862424.1"
                     /translation="MAKVPFGYEEVDERDKARRVEGVFTSVASRYDLMNDLMSLGLHR
                     LWKAFAVAKSGVRAGDRVLDIAAGSGDLARALARRAGPSGEVWLTDINRSMLETGRDR
                     ALDDGLALPAVQCDAERLPFPADHFDCVTVAFGLRNMTHKDAALAEMRRVLRPGGRVL
                     VLEFSRVWAPLARAYDWYSFNVLPSLGRVVAGDEASYRYLAESIRVHPDQETLKAMLE
                     QAGLERVEYFNLAAGVVALHVGHKF"
     gene            4341..5168
                     /locus_tag="VI319_00885"
     CDS             4341..5168
                     /locus_tag="VI319_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HEY6862425.1"
                     /translation="MKRSVLALVALVFVVLAAPQADAAGRLGGGRSLGAQRQVTPPQR
                     QATPPAQQQAQPAAPQPGGSRWLAPLAGLAAGLGLGWLFAQGGAGALLATLVLALAAG
                     ALVLVLVRALSRPRAERSPMQYAGQPVETVGSLPPSQGASRPVGGGAPGIPAGFDVDA
                     FLGQAKRNFLALQDANDRGDVDALRDVTTSEMFTALADDVRSHAGSGQSVDVVSLDAD
                     LLEVVTEGPLHWASVRFHGTVREDPRALPEGFEEVWHLQKPVSGARGWVLAGIQQPA"
     gene            5173..5796
                     /locus_tag="VI319_00890"
     CDS             5173..5796
                     /locus_tag="VI319_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY6862426.1"
                     /translation="MTAGGVLLERSAFLERAAQAALNHVLAQNPWACARLAAFAGRSV
                     ALRCPPFPELRLRILESGRVAAAGDADEASLTVTLTPAALPFLAARDEGALRHVAIEG
                     SAELAETVRSLFANLSWDVEEDLSRLRGDVLAHRLAAGGEAFLRAQREAALRLGETLA
                     EYWQDEQPLLARPEDVAAFCRAVDALRDDVERLEKRIDLLAPTRGIV"
     gene            5808..7340
                     /gene="ubiB"
                     /locus_tag="VI319_00895"
     CDS             5808..7340
                     /gene="ubiB"
                     /locus_tag="VI319_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY6862427.1"
                     /translation="MRRLFRLAIILWVALRFGLEEFALPEGSSATNRRGRAARRLLFW
                     RRLAEPRAVRLRRALETLGPIFVKFGQVLSTRRDLLPPDIADELAKLQDQVPPFAAEL
                     AVAEVERAFGRKIGEVFEQFSSVPVASASIAQVHCAVLPGGRECAVKVLRPGILPGIA
                     RDVALLDSGAFLVERLWADGRRLKPREVVAEFARHLYDELDLMREAANASQLRRNFLD
                     SPLLCVPEVYWEWCTGTVMTMQRMSGTPISQVAELRRQGVDVPRLARAGVEIFFTQVF
                     RDGFFHADMHPGNILVSSDGRYIALDFGIMGTLTDADKSYLAQNFLAFFQRDYRRVAQ
                     AHVDAGWVPADTRIDEFESAIRAVCEPVFDRPLKDISFGRVLLRLFQTSRRFNVEIQP
                     QLVMLQKTLLNIEGLGRQLDPDLDLWKTAKPFLERWMSEQVGWRGLVRNIRDEAPLWT
                     GILPRLPRMLYRALHEDRQMRVERLFEALLATERRRTRALEWMVLLLAAALAWTVFFA
                     VF"
     gene            7360..8826
                     /locus_tag="VI319_00900"
     CDS             7360..8826
                     /locus_tag="VI319_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY6862428.1"
                     /translation="MLIASVVLYLLVTIAIGLWAALRVKNSKDYVVAGRSLPLYMNTA
                     TVFATWFGAESVLSVSVEFQKSGLGGIIADPFGSSFCLVLVALFFARAFYRMDLLTIG
                     DFYRKRYGKTMELGTSVVIAISYLGWTAAQLTALGLVFSTLTGDAVSLQTGIVISGVV
                     VLAYTIWGGMWSVAMTDFFQSLMIIFGLGIIAWFVGDMAGGWGRVVAAAADAGKFQFW
                     PTGGTREWLAFVTAFVTLSIGSIPQQDIFQRVTSAKNERTAIAGSLLGGAVYFSFVFV
                     PIYIVVAALMIDPSLAQLLSSQDAREMQRILPAFILGHTPMWVQVLFFGALLSAILST
                     ASGAIIAPTSLCTENIIRPRFPHMSDRQFLLTLRIVLISFTAAAMVFALQSKQTMYDM
                     VQNAYSVTLVAALVPLAAGIFWARANNTGAILSAVFGLVAWMIAAGTAPDATVPPPLV
                     GFGFSLAGMVLGSVLPRAARPAHQDVHHAGAHETHTRG"
     gene            8878..9504
                     /locus_tag="VI319_00905"
     CDS             8878..9504
                     /locus_tag="VI319_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEY6862429.1"
                     /translation="MFGRLMPHEGRFFGYFGEHAALIVQGAKELKEMMAHLGDFELRA
                     HNIKVIEGKADHVTHQTMQLLHQTFITPLDREDIHLLITSMDDCLDLMEDVSHCMFLY
                     EVRTVPDEARRLADICVACAEKVQEAVKMLDNMANAERIMKVCGEIDRLETEADQVLR
                     AGLAKLFHSEPDTRELIKLKEIYEYLETVTDRCEDVANIIEGIVVENG"
BASE COUNT         1274 a         3474 c         3345 g         1496 t
ORIGIN      
        1 cgtgctgttc gacggcgagc gcctcggcgg cttcatcgac ttctacttcg cgggcgtcga
       61 tgcgttcctg ttcgacgtcg cggtgaccgt caacgactgg tgcatcgact cgggcggcga
      121 gctcgacgcc gagcgcggcc gcgcactgct cgacgcgtac gccggcgtgc gcgccttcac
      181 cgccgaggag cggcgcgcct ggccggcgat gctgcgcgcc ggcgcgctgc gcttctggct
      241 ctcgcgcctg tacgacttct acctgccgcg cccgggcgag ctcacgcacg cgcacgaccc
      301 cgagcatttc cgccgcgtgc tcgagtcgcg ccgcgagcgc gcgctgccgc tgccctgagg
      361 cgccgcgatg aggatcgtcc cggcgaaaaa cggcgcggcc tggctcgccc gcggcctcgt
      421 ccttttccgg cgcaatccgc cggcatggct gctcgcggtg ctcgtctact ggcttgcggt
      481 cgggctgctc gagcagctgc cgcacgtcgg gcgggcgctg tccatgctgg tgctgccgcc
      541 ctttaccatg tcgttcatgg cgctgtgcgc gctgctcgag gcgggggcgc cgctgcgccc
      601 gcgcccgctc gtcgacgcct tccgcacgcg cctgccggtc ctcgtcacgc tcggcgctct
      661 gtacctggcc tgcgtcctgg cggtgctcgc gctcgcctcg ctcgtcgacg acggcgtgct
      721 gctcgactgg gcgctcgagg gcaccgagcc gccgcccgac gcgatcgccg aggggcgcgc
      781 gtcgcgcggg ctgctcgccg gcgcgctcgg cctgacgccg gtcatgatgg cgttctggtt
      841 cgctccgccc ctcgcgggct ggggcggcat gggcgcggtg cagtcgctct tctacagctt
      901 cttcgcggcg tggcgcaact ggcggccgtt cctggtgtac ggcgcggtgc tcatgctcgg
      961 cggggtggcc gtcgtcgccg ccatgtcgat ggtcgcggtg ctcacccacg gtaacgccga
     1021 ctcggtgcac gccgcgctgc tcgtggcgac cctgctgacg ctgccgatcc tgttcggcag
     1081 cttctacgcg agctaccgcg acgtgttccc cgaagccggc gcggcatgac gcggcccatc
     1141 cgcgctcagt ctgctatgct tgacgcatgc ccgcgcgcga aggcgacacg accgccgccg
     1201 ggaacgccgc cgcgcgttgc gagctgtgcg cctcggacgg cggcgaggtc ctctggcggg
     1261 acagcttctg ccgcatcgtc ggcgtggccg acgcggacta tccgggattc tgccgcgtgg
     1321 tcctgcagcg ccatgtgaag gaaatgaccg atctcgagcg cgaggagcgg gcgcggctca
     1381 tggacgccgt gttcgcgacc gaggccgcgc tgcgcgagct gctgcgaccc gacaaggtga
     1441 acctggcgag cctcggcaac atcacccagc acctgcactg gcacgtgatc ccgcgcttcg
     1501 ccgacgaccg ccatttcccg cagccgatct gggcgcagcc ggcgcgtccg cgcgcgtcgc
     1561 gccgcgcccc ggggtccagc gcgctcgccg acgcgctgcg gcggcggctg ggcgactagc
     1621 gcgatgtcgt ccttcgacct gcccgccgcc gcgccggacg cgaatcccga tttccgcgac
     1681 gccgccggct gcgccgcctg gctgcagggc gtcccgctcg tcaacgtcgc gagctcgcac
     1741 ggcaagctca tcgcccagct cgccgagctg aaccgctgcg cgatcgcgcc ggccgagcgg
     1801 ctgaagatcc tggagcagct gcgccctgca gtgctgttcg tccaggcgga gcacgcgaag
     1861 aagttcgcga accggccggt gccgctgtcg ccgcccgagc gcgaggcgtt caacgccgtg
     1921 acctcgctct ggggcgcgtt cctggtcggc taccagtact gcctcgaggc cgagaccgat
     1981 gcggcccagg ccgcggtcgc gtgccagcgg gccctctggt gcctgagcga gaagatcggc
     2041 gagcaccacg cgacctacca ggagatcgcg cccgagaact ggaagctcct caaccgcgtc
     2101 tacgcgctcg ccgaggagcg cggcgtcgcc gagcgcgaag tggatcatcc cgaatacaag
     2161 aagatcgaga cgcgctgcgt cgagacctac gcgcgcgcgc tgctcctcaa cctggcgaat
     2221 ccgaacgagc actcgccgcg ccagacggcg gtcatcgctc gctggctcga gcgctggtcc
     2281 cagaaggtga ggatctcgcg cacgccgccc gcgggagacc aggcgccgct cgcggtcgat
     2341 ctcgcgggcg acggcggcgc cgcgcgcagc ggcgcgacgg gcgagaccgt gcgctacctc
     2401 gacgtgtccg acatcgcgcg cagcgtgaag aagcgcgtgt cgctgctgcg caagggcgag
     2461 tcgccgtcgg cgctcaatct cggcgacgtg ccaccggcga ccgcggagca gcttctcatc
     2521 atgctgttca agcagtggtg cgaggacaag cactcgcgcg tgcacccgcg ccgggcggcg
     2581 agcggcacgg ccgatctctg catcggcatg ccggccatgc acttctacgt ctcgggcgag
     2641 cgcttccggc agcccgccgg gctcgaggag ctctcgaaga tgcagcgcga ggagatcgcc
     2701 accttcggcc gcgtggccac ccgccacgag gaggaggact acgccaagac gcaagggttc
     2761 ttcctcgaga cctggcagct gaaggacgag agccgctcgg ggctgcgtct cgagcggccc
     2821 gaccgttccg cggccgcgcg ctaccagctg caccagctca tcgcggtgcg tcccgccgac
     2881 ggcaagagct tcatcctcgg catggtgcgc tggctctcgg tcgctcccga cctgctgctg
     2941 cgcgccggcg tgcgcatcat gccgggcatg cccaagggcg tctcggtacg cggcaccggg
     3001 ctgaacgcga ccaacgagcg cttcaccgcc gcgctgctcc tgcccgcggc ggccgcgctc
     3061 ggctcgcccg agtcgctggt gatcccgaac ggctggttca agccgaatcg cgtgatcgag
     3121 ctgatccgcg aatcctcgtc gaacgtcgtc ctcaccgcgc tgctcgagcg cggcgtcgat
     3181 tacgagcgcg tgaccttcga gcccgcgtag ccgcgcgggt cgcagagcgt cgcatgggcc
     3241 gcgttcccac cgagatcaag ctgcacagca agtcccgcat catggagatc acgttcgacg
     3301 acggggagcg cttcgagctc tcctacgagt tcctgcgggt gtattccccc tcggccgagg
     3361 tgcgcggcca cgggccgggc caggaggtgc tgcaggccgg caagaaggac gtcgacatcg
     3421 ttgcactcga gccggtcggg acctacgcgg tgcagccccg gttctccgac ggccacgcga
     3481 ccggcatcta ttcctgggac tatctccatg agctcgggac gaaccgcgac gcgctgtggc
     3541 aggattacct cgagcgcctg cgccggtccg gcgcgcggcg cgagccctga tcgccatggc
     3601 caaggtcccc ttcggttacg aggaagtcga cgagcgcgac aaggcgcgcc gcgtcgaggg
     3661 cgtgttcacg tcggtcgcgt cgcgctacga cctcatgaac gacctgatgt cgctcggcct
     3721 gcaccggctg tggaaggcct tcgccgtggc gaagagcggc gtgcgcgcgg gcgatcgcgt
     3781 gctcgacatc gcggcgggct cgggcgacct cgcgcgggcg ctcgcgcgcc gcgccgggcc
     3841 ctcgggcgag gtctggctga ccgacatcaa ccgctcgatg ctcgagacgg ggcgcgaccg
     3901 cgcgctcgac gacggactcg cgctgcccgc ggtgcaatgc gacgccgagc ggctgccctt
     3961 tcccgccgat catttcgact gcgtgacggt cgcgttcggg ctgcgcaaca tgacgcacaa
     4021 ggacgcggcg ctcgcggaga tgcggcgcgt gctgcgcccc ggggggcgcg tcctcgtgct
     4081 cgagttctcg cgcgtgtggg cgccgctcgc gcgcgcgtac gactggtact cgttcaacgt
     4141 gctgccgagc ctgggccggg ttgtggcggg cgacgaggcg agctatcgtt atctcgccga
     4201 gtcgatccgc gtgcatcccg accaggagac gctcaaggcc atgctggaac aggccgggct
     4261 cgagcgggtc gaatacttca atctcgccgc cggcgtcgtc gccctgcacg tcggccacaa
     4321 gttctgacag gaggctcatc gtgaaacgat ccgtgctggc tctggtcgcg ctcgtgttcg
     4381 tcgtcctcgc cgcgccgcag gccgatgccg cgggccgtct cggcggcggc cgcagcctgg
     4441 gcgcccagcg ccaggtgacc ccgccgcagc ggcaggcgac cccgcccgcg cagcagcagg
     4501 cgcagccggc cgcgccgcag cccggcggct cgcgctggct cgctccgctc gcgggcctcg
     4561 ccgccgggct cgggctcggc tggctgttcg cacagggcgg cgccggcgcg ttgctcgcga
     4621 cgctcgtgct cgcgctcgcc gcgggcgcgc tcgtcctggt gctcgtgcgc gcgctgtcgc
     4681 ggccgcgcgc cgagcgctcg ccgatgcaat acgccggcca gccggtcgag accgtcggct
     4741 cgcttccgcc atcgcagggg gcttcgcgcc ccgtcggggg cggcgctccc ggcatcccgg
     4801 ccggattcga cgtcgacgcc ttcctcggcc aggcgaagcg caatttcctc gcgctgcagg
     4861 acgcgaacga ccgcggcgac gtcgatgcgc tgcgcgacgt gacgacgagc gagatgttca
     4921 cggcactcgc cgacgacgtg cgctcgcacg cgggctcggg ccagagcgtc gacgtggtga
     4981 gcctcgacgc cgacctgctc gaggtggtca cggaggggcc gctgcattgg gcgagcgtcc
     5041 gcttccacgg caccgtgcgc gaggacccgc gcgcgctgcc cgaagggttc gaggaagtct
     5101 ggcacctgca gaaaccggtc agcggcgcgc gcggctgggt gctcgcgggc atccagcagc
     5161 cggcctgaag cgatgacggc gggcggcgtc ctgctcgagc gcagcgcttt tctggagcgc
     5221 gccgcgcagg ccgcgctcaa ccacgtcctc gcgcagaacc cgtgggcgtg cgcgcgcctc
     5281 gcggcgttcg ccgggcgcag cgtcgcgctg cgctgcccgc ccttccccga gctgcggctg
     5341 cgcatcctgg aatcgggccg ggtcgccgcc gcgggcgacg cggacgaagc ttccctgacc
     5401 gtgacgctca cgcccgcggc gctgccgttc ctcgccgcgc gcgacgaggg cgcgctccgg
     5461 cacgtcgcga tcgaggggtc ggcggagctc gcggagaccg tgcgctcgct cttcgcgaac
     5521 ctctcctggg acgtcgagga ggacctctcg aggctgcgcg gcgacgtgct cgcacaccgc
     5581 ctcgccgccg gcggcgaggc cttcctgagg gcgcagcgcg aggccgcgct gcgcctgggg
     5641 gagaccctcg ccgagtactg gcaggacgag cagccgctcc tcgcccgtcc cgaggacgtc
     5701 gccgcgttct gccgcgcggt cgacgcgctg cgcgacgacg tcgagcgcct cgagaagcgg
     5761 atcgatcttc tcgccccgac ccggggaatc gtgtagattc cggcgacttg cgccgcctct
     5821 tccgtctcgc aatcatcctc tgggtcgccc tgcgcttcgg gctggaggaa ttcgcgctcc
     5881 ccgagggctc gtccgcgacg aaccggcgcg gacgcgccgc gcggcggctg ctgttctggc
     5941 gccggctcgc ggagccgcgc gccgtgcggc tgcggcgcgc gctcgagacg ctcggcccga
     6001 tcttcgtcaa gttcggccag gtgctgtcga cgcggcgcga cctgctgccg cccgacatcg
     6061 ccgacgagct cgcgaagctc caggaccagg tgccgccgtt cgcggccgag ctcgcggtgg
     6121 ccgaggtcga gcgcgccttc gggcgcaaga tcggcgaggt gttcgagcag ttctcgagcg
     6181 tgccggtggc gagcgcgtcg atcgcgcagg tgcactgcgc ggtgctgccg ggcgggcgcg
     6241 agtgcgcggt caaggtgctg cgccccggca tcctgcccgg catcgcgcgc gacgtcgcgc
     6301 tgctcgactc gggcgccttc ctggtcgagc ggctctgggc cgacggcagg cggttgaagc
     6361 cgcgcgaggt ggtcgccgag ttcgcgcggc acctctacga cgagctcgac ctcatgcgcg
     6421 aggccgcgaa cgcgagccag ctgcggcgca acttcctcga ctcgccgctg ctgtgcgtgc
     6481 ccgaggtgta ctgggagtgg tgcacgggca cggtgatgac catgcagcgc atgagcggca
     6541 cgccgatcag ccaggtggcc gagctgcgcc gccagggcgt cgacgtcccg aggctggcgc
     6601 gcgccggcgt cgagatcttc ttcacccagg tgttccgcga cggcttcttc cacgccgaca
     6661 tgcaccccgg gaacatcctc gtgagttcgg acggccgcta catcgcgctc gacttcggca
     6721 tcatgggcac gctcaccgac gccgacaaga gctacctcgc gcagaacttc ctcgccttct
     6781 tccagcgcga ctaccgccgc gtggcgcagg cgcacgtcga cgccggctgg gtgcccgccg
     6841 acacgcgcat cgacgagttc gagtcggcga tccgcgccgt gtgcgagccg gtgttcgacc
     6901 ggccgctcaa ggacatctcg ttcggccgcg tgctgctgcg cctgttccag acctcgcgcc
     6961 gcttcaacgt cgagatccag ccgcagctgg tgatgctgca gaagacgctg ctcaacatcg
     7021 agggcctggg gcgccagctc gatcccgacc tcgacctgtg gaagaccgcg aagcccttcc
     7081 tcgagcgctg gatgagcgag caggtcggct ggcgcggcct ggtgcgcaac atccgcgacg
     7141 aggcgccgct gtggaccggc atcctcccgc ggctgccgcg catgctgtac cgcgcgctgc
     7201 acgaggaccg gcaaatgcgc gtggagcgcc tcttcgaggc gctccttgcc acggagcgcc
     7261 ggcgcacccg cgcgctcgag tggatggtgc tgctgctcgc cgccgcgctc gcctggacgg
     7321 tgttcttcgc cgtgttctag cgccttataa tcgccgccca tgctgatcgc gtccgtcgtc
     7381 ctctacctcc tcgtcaccat cgcgatcggc ctgtgggccg cgctgcgcgt caagaactcc
     7441 aaggactacg tcgtcgccgg ccgcagcctg ccgctctaca tgaacacggc caccgtgttc
     7501 gccacgtggt tcggcgcgga gagcgtgctc tcggtgtcgg tcgagttcca gaagagcggg
     7561 ctggggggca tcatcgccga ccccttcggg tcctccttct gcctggtcct cgtcgcgctc
     7621 ttcttcgcgc gcgccttcta ccgcatggac ctgctcacca tcggcgattt ctaccgcaag
     7681 cgctacggca agaccatgga gctcggcacg agcgtcgtca tcgcgatctc gtacctcggc
     7741 tggacggccg cgcagctgac cgcgctcgga ctggtgttct cgaccctcac cggggacgcg
     7801 gtgtcgctgc agaccggcat cgtcatcagc ggcgtggtgg tgctcgcgta caccatctgg
     7861 ggcggcatgt ggtcggtcgc gatgaccgac ttcttccagt ccctcatgat catcttcggg
     7921 ctgggcatca tcgcctggtt cgtcggcgac atggccggcg gctggggccg ggtcgtggcg
     7981 gcggcggcgg atgccggcaa gttccagttc tggccgaccg gcgggacccg ggaatggctc
     8041 gccttcgtca cggcgttcgt gacgctctcg atcggctcga tcccgcagca ggacatcttc
     8101 cagcgggtca cgtcggcgaa gaacgagcgc accgcgatcg cggggagcct gctcggcggg
     8161 gcggtctact tctcgttcgt gttcgtcccg atctacatcg tcgtggccgc gctgatgatc
     8221 gacccgtcgc tcgcgcagct gctgtcgtcg caggacgcgc gcgagatgca gcgcatcctc
     8281 cccgcgttca tcctcgggca cacgccgatg tgggtgcagg tgctgttctt cggcgcgctg
     8341 ctctcggcca tcctctccac ggcgagcggc gcgatcatcg cgcccacgtc gctgtgcacc
     8401 gagaacatca tccggccgcg cttcccgcac atgagcgacc gccagttcct gctgacgctc
     8461 aggatcgtgc tgatctcctt taccgccgcg gcgatggtgt tcgccctcca gtcgaagcag
     8521 accatgtacg acatggtgca gaacgcgtac agcgtgacgc tggtggccgc gctcgtgccg
     8581 ctcgcggcgg gcatcttctg ggcgcgggcc aacaacacgg gggcgatcct gtcggccgtg
     8641 ttcggcctgg tcgcgtggat gatcgccgcg gggaccgcgc ccgacgccac ggtgccgccg
     8701 ccgctggtcg ggttcgggtt ttccctcgcc ggcatggtgc tcgggtcggt cctgccgcgc
     8761 gcggcgcggc cggcgcacca ggacgtgcac cacgccggcg cgcacgaaac gcacacccgc
     8821 ggctgaaagc gcggctaaaa tgcgcgcgcc cttcccccac gactgctcga ggtcgccatg
     8881 ttcggacgcc tgatgcccca cgaaggccgc ttcttcggat atttcggaga gcacgccgcg
     8941 ctgatcgtcc agggcgcgaa ggagctcaag gagatgatgg cccacctcgg cgacttcgag
     9001 ctgcgcgccc acaacatcaa ggtgatcgag ggcaaggccg accacgtcac gcaccagacg
     9061 atgcagctgc tgcaccagac cttcatcacg ccgctcgacc gcgaggacat ccacctgctg
     9121 atcacctcga tggacgattg cctcgacctc atggaggacg tgtcgcactg catgttcctg
     9181 tacgaggtgc gcacggtccc ggacgaggcg cggcgcctcg ccgacatctg cgtcgcctgc
     9241 gccgagaagg tccaggaggc ggtgaagatg ctcgacaaca tggcgaacgc cgagcgcatc
     9301 atgaaggtct gcggcgagat cgaccggctc gagaccgagg ccgaccaggt gctgcgcgcg
     9361 ggcctcgcga agctgttcca ttccgagccc gacacgcgcg agctgatcaa gctcaaggag
     9421 atctacgagt acctcgagac ggtgaccgac cgctgcgagg acgtggcgaa catcatcgaa
     9481 ggcatcgtcg tcgaaaacgg ttagcgccgg cacgcgccaa tccgaccgcc ggcacccgcc
     9541 caaccaagaa aagaacagac gacgtcccga tgagcaccga gatcctgat
//
LOCUS       DATDMW010000028         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1998041, whole genome shotgun sequence.
ACCESSION   DATDMW010000028 DATDMW010000000
VERSION     DATDMW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998041"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..780)
                     /gene="gloB"
                     /locus_tag="VI319_00910"
     CDS             complement(4..780)
                     /gene="gloB"
                     /locus_tag="VI319_00910"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY6862430.1"
                     /translation="MADAKIQVVPLRAFKDNYVWTLRNDRHAAVVDPGEARPVLDYLA
                     SEGLKLSAILATHHHQDHVGGVAGLLERHSCPVYGPRGEPIATLTHPVGGGDTVTLPE
                     LGLSLEVLDIPGHTRAHIAYYGAKLLFCGDTLFACGCGRVFEGTPAQMHASLQKLAAL
                     PDDTLVYCGHEYTLANIGFAQRVEPGNPQLLAREASDAKLRERGLPTLPSTMGREKAT
                     NPFLRVREPAVVDAASKYLGHRAGDAAGVFAALREWKNRS"
     gene            818..1555
                     /locus_tag="VI319_00915"
     CDS             818..1555
                     /locus_tag="VI319_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6862431.1"
                     /translation="MLSKWFHTPAGRYILDWEQGQFDSAVDDVFGYNAVQIGLPEIDF
                     LRQSRIVLKVRAGVDAGAGLVTEAGRLPLASQSIDLVVLPHVLEFAEQPHQILREAER
                     VLMPEGSIVISGFNPLSLWGAKRAFVRRRPERPVPWCGKFLGLLRLKDWLSLLGFELN
                     GGRFGCYAPPFTQTRWLQRFQFMEKAGDRWWPVLGGIYVVRAVKHTVGMRLVVPNWRD
                     KRKRAEALAPVAQKDNGVHVKQRRRDG"
     gene            1548..1988
                     /gene="rnhA"
                     /locus_tag="VI319_00920"
     CDS             1548..1988
                     /gene="rnhA"
                     /locus_tag="VI319_00920"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY6862432.1"
                     /translation="MADPVRIYADGACRGNPGPGGWGAILRYGTEEKEIFGGEPQTTN
                     NRMELTAVIRALEMLKRPSVVEVYTDSQYVQKGVSEWLADWKRRGWKTADKKPVKNRE
                     LWEELDRMRAQHRVTWHWVRGHAGHPENERADALANRGVDSLSR"
BASE COUNT          332 a          755 c          777 g          324 t
ORIGIN      
        1 ggatcaagac ctgttcttcc actcgcgcag ggcggcgaaa acgcccgccg cgtcgcccgc
       61 ccggtgcccc agatacttgc tcgccgcgtc cacgaccgcc ggctcgcgca cgcgcaggaa
      121 ggggttggtc gccttctcgc ggcccatcgt cgacgggagg gtcggcaacc cgcgctcgcg
      181 cagcttcgcg tcgctggcct cgcgcgcgag cagctgcgga ttccccggct cgacccgctg
      241 ggcgaagccg atattcgcga gggtgtactc gtgtccgcag tagacgagcg tgtcgtcggg
      301 cagggcggcc agcttctgca gcgacgcgtg catctgcgcc ggcgtgcctt cgaagacccg
      361 gccgcagccg cacgcgaaga gcgtgtcgcc gcagaacagg agctttgccc cataataggc
      421 gatgtgcgcg cgcgtgtgcc ccgggatgtc gagcacctcg agcgagagcc cgagctcggg
      481 aagggtcacc gtgtcgccgc cgccgaccgg atgcgtcagc gtcgcgatcg gctcgccgcg
      541 cggcccgtag accgggcagg aatgccgctc cagcagcccg gcgacgccgc cgacgtggtc
      601 ctggtgatga tgcgtggcca ggatcgcgga gagcttcaat ccttcggaag cgagataatc
      661 cagcaccgga cgcgcctcgc ccgggtcgac gaccgcggcg tggcggtcgt tgcgcagcgt
      721 ccagacgtag ttgtccttga acgcccggag gggaacgact tgaatctttg cgtcggccat
      781 tgagcggatc attgtgttcg agcccagcga aaagtcaatg ctctcgaaat ggttccacac
      841 cccggcgggg cgctacatcc tcgactggga gcagggccag ttcgacagcg cggtcgacga
      901 cgtgttcggc tacaacgcgg tccagatcgg gctgcccgag atcgacttcc tgcgccagtc
      961 tcgcatcgtg ctcaaggtgc gcgcgggcgt cgacgccggc gccggcctgg tcaccgaggc
     1021 gggccgcctg ccgctcgcct cgcagagcat cgacctcgtg gtgctcccgc acgtcctcga
     1081 gttcgccgag cagccgcacc agatcctgcg cgaggccgag cgcgtgctca tgcccgaagg
     1141 ctcgatcgtc atctccggct tcaaccccct gtcgctgtgg ggcgcgaagc gcgccttcgt
     1201 ccgccgccgg cccgagcgtc ccgtgccctg gtgcggaaag ttcctggggc tgctgcgtct
     1261 caaggactgg ctgtcgctgc tcggcttcga gctgaacggc ggccgcttcg gctgctacgc
     1321 gccgcccttc acgcagacgc gctggctgca gcgcttccag ttcatggaga aggcgggcga
     1381 ccgctggtgg ccggtgctcg gcgggatcta cgtcgtgcgc gcggtcaagc acaccgtcgg
     1441 catgcggctg gtcgtcccga actggcgcga caagcgcaag cgcgccgagg cgctcgcgcc
     1501 ggtggcgcag aaggacaacg gggtgcacgt caagcagcgc cggcgcgatg gctgacccgg
     1561 tccggatcta cgcggacggc gcctgccgcg gcaatcccgg gcccgggggc tggggcgcga
     1621 tcctgcgcta cggcaccgag gagaaggaga tcttcggcgg cgagccgcag accaccaaca
     1681 accgcatgga gctcaccgcg gtcattcgcg cgctcgaaat gctgaagcgc ccgtcggtcg
     1741 tcgaggtcta caccgactcg cagtacgtcc agaagggcgt gagcgagtgg ctcgcggact
     1801 ggaagcgccg cggctggaaa acggcggaca agaagccggt gaagaaccgc gagctctggg
     1861 aggagctcga ccggatgagg gcccagcacc gcgtcacctg gcactgggtc cgcggccacg
     1921 ccggccaccc cgagaacgag cgcgccgacg cgctcgccaa ccgcggcgtc gattcgctct
     1981 cccgctagag cgcgttgcgc ggcgggaccg atttgtgcaa tatggcccga aaagcggagg
     2041 cgggagtcat gacgacggcg cttctgatca tccacgggct gctcgccgtc gcgctgctcg
     2101 gcgcgatcac ccaccaggcg gcgagcgtgt ggctgcccgg gcgcaggccg gcggggtcct
     2161 tcgtgggacg cttccggtcg gtgcccgc
//
LOCUS       DATDMW010000029         6374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1002874, whole genome shotgun sequence.
ACCESSION   DATDMW010000029 DATDMW010000000
VERSION     DATDMW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002874"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VI319_00925"
     CDS             complement(<1..1153)
                     /locus_tag="VI319_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6862433.1"
                     /translation="MIRLSNVTLRRGAKALLEGADAALAPGDKIGLIGANGSGKSSLL
                     ALLRGELHADAGDVDLPARWRIAHVAQETPALERPAIEYAIDGDLELRKLEAALAQAE
                     AADDGHAIGELHAMLRDADAYTVRPRAERLLRGLGFARDELARPVSSFSGGWRMRLNL
                     AQALMCPSDLLLLDEPTNHLDLDAILWLEDWLRRYAGTLIVISHDRDFLDGAVGAIVH
                     IDGCKLRRYAGNYSAFERQRAAAIVLAAAQAEKQARERAHLESFIDRFRAKATKARQA
                     QSRMKQLERMEVLAPLHVSAPFSFEFREPLKSPNPLLVFEDVSAGYGERVVLRGVTLS
                     LQSGQRIGLLGVNGAGKSTFVKSVAGVLAPLSGKATLNKGLEIGYFAQHQ"
     gene            1200..2249
                     /locus_tag="VI319_00930"
     CDS             1200..2249
                     /locus_tag="VI319_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6862434.1"
                     /translation="MAATRRILVLLLVLAASAGALAQDREARNRELYQYRGADREQRL
                     VAAARKEGGLTLYSTMTPEDAAPLFAGFEKKYGVKVTMWRGINQKLVQRALAEARAGR
                     PAADAFEGSGHGMEILYREGLLEPFWSPAFADIPADAFPSHRGYAPDNLLFFVMGYNT
                     RLVKPEDVPRSYDDLLAPRWAGNLGIEASDVVWFAAVANAMGKPKGLAYFERLAATRP
                     QVRNGHTLMTELVAAGEIPIALTLYNQAVDKLKEKGAPIDWKPLPPAFGRADGIALAK
                     QARHPSAALLFADYLLSPEGQRFIAQASRVPVNRRVETGFPKDAFRVIDMSTMMDEWN
                     DWEKRFETLFLKGHP"
     gene            2246..3058
                     /locus_tag="VI319_00935"
     CDS             2246..3058
                     /locus_tag="VI319_00935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6862435.1"
                     /translation="MSAAAGHPRIRFGGDCEGVLFLPAGAARAGAVVILHERYGLVKH
                     TLDLGERLARDGYVALAPDLFSRWSGDKAALARGEARAALPDDEVAAVVDAAIDFLKT
                     HPRVAAARIAVMGVCQSGRYPIVVGSRRRDVAALVVFYGASQRRDWDASPVQPRSMAE
                     MLPAIAAPALFVFGERDHTISLADVRSVRDVLEEGRRSYRMKIFAGMPHGWLNDTMPG
                     RYREKEAAEAWKLLLAFLDDVFSARWPGGQVRWEFESATAPDYDFSRNVRLE"
     gene            complement(3059..4501)
                     /locus_tag="VI319_00940"
     CDS             complement(3059..4501)
                     /locus_tag="VI319_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862436.1"
                     /translation="MTTTLRAVLAKSAISACTLWCAAAADAQQYSGQEEARNLRLVGF
                     HDLAARSAYQPTIRHQGERWILYVGHHGGRAVNPLTGKLEENGTSVLDVTDPAAPRLL
                     AHIPGEKGREVPGRETGGAQMTRVCLGSELPKGDPRKVYLLRNFGDSRDEIWDVSTPE
                     KPALVSQFGKFKSTHKNEWECASGIAYLPGSDPAYRASRVTHVYDLSDPAKPRFIRTF
                     GLIDQLKDAKGYQPGQIHGPISAYPARERVYFGYGTNARGVAQVVDRQKLLKGAPEPA
                     PPNLLDPQVVRINLPEFMGAHTAFPLLDVELPDLKAGEKKKRDFLVIVNENNVTVCNE
                     PRQMAFFVDITDEKRPLGVASYDVLESSGGFCARGGRFGAHASNESTAPVYYRKLMFF
                     SWFNAGVRMVDVRDPYRPKEVGYYIPARTPKTDLRCADEAKKTGCVPVIQINNVEIDE
                     RGYVYAVDRANSGLFVLEPTGEAREIAGLR"
     gene            4714..4920
                     /locus_tag="VI319_00945"
     CDS             4714..4920
                     /locus_tag="VI319_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862437.1"
                     /translation="MDSRLTTEEVNLMRAMRDIHSGPVKVPAHIAPKLKLLGYVTAQG
                     EYCSLTMKGRDELIDRERDAARMN"
     gene            complement(5111..5350)
                     /locus_tag="VI319_00950"
     CDS             complement(5111..5350)
                     /locus_tag="VI319_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862438.1"
                     /translation="MSKKAIILVLALAGASTVATAQQPPPQGKPTFEERKQHMLERMD
                     QRIQAMQKARDCLAQAQDQQAARACRQHGPGQERH"
     gene            5786..>6374
                     /locus_tag="VI319_00955"
     CDS             5786..>6374
                     /locus_tag="VI319_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY6862439.1"
                     /translation="MFAGGALAQSVTAPTNIEIYGHLDLSIDDATKGIAGHLDGSGAP
                     LQGKLGWQPDISSNLSRFGVRGGHDLGEGGLKAVFQIETQVDVSATPGTASSNSSSDN
                     NVKGAFASRNSFLGMAGDFGAVKLGKTDAPYKLSTGRMDPFSATVGDYNSIMGNTGGD
                     NRAEFDTRLSHAAWYESPKFGAFSFSALFAPGQNRS"
BASE COUNT          974 a         2164 c         2217 g         1019 t
ORIGIN      
        1 cctggtgctg cgcgaagtag ccgatctcga gtcctttgtt cagcgtcgcc ttgcccgaga
       61 gcggcgcgag gaccccggcg acgctcttca cgaaggtcga cttccccgcg ccgttgaccc
      121 cgagcaggcc gatccgctgc ccggactgga gcgacagcgt cacgccgcgc agcacgacgc
      181 gctcgccgta gcccgccgac acgtcctcga acacgagcag cgggttgggg ctcttcagcg
      241 gctcgcggaa ctcgaacgag aacggcgccg acacgtgcag cggcgcgagg acttccatcc
      301 gttcgagctg tttcatgcgg ctctgcgcct ggcgcgcctt cgtggccttg gcgcggaagc
      361 ggtcgatgaa ggactcgagg tgagcgcgct cgcgcgcctg cttctcggcc tgcgccgcgg
      421 cgagcacgat cgcggcggcg cgctggcgct cgaacgccga atagttgccg gcgtagcgcc
      481 tgagcttgca gccgtcgatg tgcacgatgg cgccgaccgc gccgtcgagg aagtcgcggt
      541 cgtgcgagat cacgatcagc gtgccggcgt agcggcgcag ccagtcctcg agccacagga
      601 tggcgtcgag gtcgagatgg ttggtcggct cgtcgagcag cagcaggtcc gacgggcaca
      661 tcagcgcctg cgccagattg agccgcatgc gccagccgcc ggagaagctc gagaccggac
      721 gcgcgagctc gtcgcgcgcg aagccgaggc cgcggagcag ccgctcggcg cgcgggcgca
      781 cggtatacgc gtcggcgtcg cggagcatcg cgtgcagctc gccgatcgca tggccgtcgt
      841 cggcagcttc ggcttgcgcg agcgcggcct cgagcttcct caattccagg tcgccgtcga
      901 tcgcatactc gatcgccgga cgctcgagcg cgggcgtctc ctgcgcgaca tgcgcgatgc
      961 gccagcgcgc gggcaggtcg acgtcgccgg cgtcggcgtg cagctcgccg cgcagcagcg
     1021 cgagcaggct cgacttgccg gagccgttgg cgccgatcag gccgatcttg tcgccggggg
     1081 cgagcgccgc gtcggcgcct tcgagcagcg ccttcgcgcc gcggcgcagc gtgacgttgg
     1141 agagacggat catcgcgaaa ccgcgcagta tatccgctcc gcgtctagaa tcgagcgcca
     1201 tggcggccac acggcgcatc ctcgtgctgc tgctcgtcct cgcggcgagc gcgggcgcgc
     1261 tcgcgcagga ccgcgaggcg cgcaaccggg agctctacca gtaccgcggc gccgaccgcg
     1321 agcagcggct tgtcgcggcg gcgagaaagg aaggcgggct cacgctctac tccacgatga
     1381 cgcccgagga cgcggcgccg ctcttcgccg gcttcgagaa gaagtacggc gtcaaggtga
     1441 cgatgtggcg gggcatcaac cagaagctgg tgcagcgcgc gctcgccgag gcgcgcgcgg
     1501 gcagacccgc cgccgacgcc ttcgagggca gcggtcacgg catggagatc ctgtaccgcg
     1561 agggactgct cgagccgttc tggagcccgg cgttcgccga catcccggcc gacgccttcc
     1621 cgagccaccg gggctacgcg cccgacaacc tgctcttttt cgtaatgggc tacaacacgc
     1681 ggctggtgaa acccgaggac gtgcccaggt cctacgacga tctgctcgcg ccccggtggg
     1741 cgggcaacct cggcatcgaa gcctccgacg tcgtctggtt cgcggccgtc gccaacgcga
     1801 tgggcaagcc gaagggcctc gcatatttcg agcgcctcgc cgcgacgcgg ccgcaggtgc
     1861 gcaacggcca tacgctgatg accgagctgg tcgccgcggg cgagatcccg atcgcgctca
     1921 cgctctacaa ccaggcggtc gacaagctga aggagaaggg cgcgccgatc gactggaagc
     1981 cgctgccgcc cgcgttcggg cgcgccgacg gcatcgcgct cgcgaagcag gcgcgccacc
     2041 cgagcgctgc gctgctcttc gccgactacc tgctctcgcc cgagggccag cgcttcatcg
     2101 cgcaggcgag ccgagtgccc gtgaaccgca gggtcgagac cggcttcccg aaggacgcgt
     2161 tccgcgtcat cgacatgtcg acgatgatgg acgaatggaa cgactgggag aagcgcttcg
     2221 agacgctgtt cctgaagggc cacccgtgag cgcagccgcc ggtcatccga ggatccgctt
     2281 cggcggagac tgcgagggcg tgctgttcct cccggccggc gcggcgcgcg ccggcgcggt
     2341 cgtgatcctg cacgagcgct acggcctggt gaagcatacg ctcgacctcg gcgagcggct
     2401 cgcgcgcgac ggctacgtcg cgctcgcgcc cgacctgttc tcgcgctgga gcggcgacaa
     2461 ggccgcgctc gcccgcggcg aggcgcgcgc cgcgctgccc gacgacgagg tcgccgcggt
     2521 cgtcgacgcg gcgatcgatt tcctcaagac gcacccgcgc gtggcggcgg cgcgcatcgc
     2581 ggtgatgggc gtgtgccaga gcgggcgcta cccgatcgtg gtcggcagcc gccggcgcga
     2641 cgtcgcggcg ctcgtcgtct tctacggcgc ctcgcagcgg cgcgactggg acgcctcgcc
     2701 cgtgcagccg cgctcgatgg ccgagatgct gcccgcgatc gcggcgccgg cgctgttcgt
     2761 cttcggcgag cgcgatcaca ccatctcgct cgccgacgtg cgcagcgtgc gcgacgtgct
     2821 ggaagaaggc cggcgcagct accgcatgaa gatcttcgcc ggcatgccgc acggctggct
     2881 caacgacacc atgccgggac gctaccgcga gaaggaggcg gcggaggcgt ggaagctgtt
     2941 gctcgcgttc ctcgacgacg tcttctccgc gcgctggccc ggagggcagg tgcgctggga
     3001 gttcgagagc gcgacggcgc ccgactacga cttctccagg aacgtccgtc tcgagtagct
     3061 agcgaaggcc cgcgatctcg cgcgcctcgc cggtcggctc gagcacgaac agcccgctgt
     3121 tcgcgcgatc gaccgcgtac acgtagccgc gctcgtcgat ctcgacgttg ttgatctgga
     3181 tcaccggcac gcagcccgtc ttcttcgcct cgtcggcgca gcgcaggtcg gtcttcggcg
     3241 tgcgcgccgg gatgtagtag ccgacctcct tcggccggta cggatcgcgc acgtcgacca
     3301 tgcgcacgcc ggcgttgaac caggagaaga acatgagctt gcggtagtag acgggcgccg
     3361 tgctctcgtt cgacgcgtgc gcgccgaagc ggccgccgcg cgcgcagaag ccgcccgagg
     3421 actcgagcac gtcgtagctc gccacgccga gcgggcgctt ctcgtcggtg atgtcgacga
     3481 agaacgccat ctgccgcggc tcgttgcaca cggtcacgtt gttctcgttc acgatcacga
     3541 ggaagtcgcg cttcttcttc tcgccggcct tcaggtcggg gagctcgacg tcgagcagcg
     3601 gaaaggccgt gtgcgcgccc atgaactcgg gcaggttgat gcgcaccacc tgcggatcga
     3661 gcaggttcgg cggggcgggc tcgggtgcgc ccttcagcag cttctgccgg tcgacgacct
     3721 gcgcgacgcc gcgtgcgttg gtgccgtagc cgaagtacac gcgctcgcgc gccgggtacg
     3781 ccgagatcgg cccgtggatc tggccgggct ggtagccctt cgcgtccttg agctggtcga
     3841 tgaggccgaa ggttcggatg aagcgcggct tcgcggggtc ggagaggtcg tagacgtgcg
     3901 tgacgcgcga ggcgcggtac gccggatcgc tgcccgggag ataggcgatg ccgctcgcgc
     3961 actcccattc gttcttgtgc gtgctcttga acttcccgaa ctgagagacg agcgcgggtt
     4021 tctccggcgt gctcacgtcc cagatctcgt cgcgcgagtc gccgaagttg cgcagcaggt
     4081 acaccttcct cggatcgccc ttcgggagct cgctgccgag gcacacgcgc gtcatctgcg
     4141 cgccgcccgt ctcgcgcccg ggcacctcgc ggcccttctc gccgggaatg tgcgcgagca
     4201 gccgcggcgc ggcgggatcg gtcacgtcga ggaccgacgt gccgttctcc tcgagcttgc
     4261 ccgtgagcgg gttgaccgcg cgcccgccgt ggtggccgac gtagaggatc cagcgctccc
     4321 cctggtgcct gatcgtcggc tggtaggcgc tgcgcgcggc gaggtcgtgg aagccgacca
     4381 ggcgcaggtt gcgcgcctct tcctggcccg aatactgctg ggcgtcggcg gccgcggcgc
     4441 accagagcgt gcacgccgag atcgccgatt tcgcgagcac cgcgcgaagc gtcgtcgtca
     4501 tggggtctcc tcccgccgcg atgataaccg gtggcgcgaa ctgtcgtctc cgggagacgc
     4561 cggcctcgcg tggcggcggg aatttgccgt gaaacggcaa aatgtgccgc gccgcgtacg
     4621 ccgcaacgtc gtacggtatg acacttggat agccgttgct gggacggctt ctgttcggcc
     4681 caatgacaga aaacgtctga gaaggagcga gcaatggaca gcagactgac caccgaggaa
     4741 gtcaacctca tgcgcgccat gcgcgacatc cactccgggc ccgtgaaggt gccggcccac
     4801 atcgcgccga agctcaagct gctcggctac gtcaccgccc agggcgagta ctgctcgctc
     4861 accatgaagg ggcgcgacga gctgatcgat cgcgagcgcg acgcggcccg gatgaactga
     4921 aggccctgga gaaacaaaaa ggcgcggctt tcgccgcgcc tttttgtttt cctgctgctt
     4981 ccgggtcttg cctcctcgtg gtctcgcttc ttctttcgag cgaagcggaa gggcccgcca
     5041 gggaaggggg agcgggccct tcgcccgctc gggatgccgg accggggagc cggaccgtca
     5101 ccccaactgc tcagtggcgc tcctgacccg gcccgtgctg ccggcacgcg cgcgcggcct
     5161 gctggtcctg cgcctgggcg aggcagtcac gcgctttctg catggcctgg atgcgctggt
     5221 ccatgcgctc gagcatgtgc tgcttgcgct cttcgaacgt gggcttgcct tgcggcggcg
     5281 gctgctgtgc cgtcgcgacg gtgctcgcgc cggcgagcgc gagcaccagg atgatcgcct
     5341 tcttgctcac caaatctccc tgcaaaattg tcgatgcgcc gattttgcgc gggtgaatgc
     5401 agggaagctg caacaattgg tatccaaatg ttatcgagcc gcgcacgttg agcgtctcgc
     5461 caacactgag cgtctcggca acactgcggg tttaccttgg cggaagcgcc ggctcacgcg
     5521 cgtgctcgtg ctccactagg ggtttacccg cgatgcagcg cttcagccgt tgtcagccaa
     5581 acgtcatcga atcgcaaggt atctcgcgag gcgcggactt aacatttttc tcgccgcgaa
     5641 aaattccaca gcggtaagtt cggcggcgag gggttctgaa gttcctcttg cgcgccgttg
     5701 atgtcatgcg gagagagagg agatgcgtat gagaaccaga tggggagcag cagccgttgc
     5761 ccttgcgctc ggctcgacag ggggcatgtt cgcgggcggc gcgctcgcgc agtcggtgac
     5821 ggcgcccacc aacatcgaaa tatacggcca tctcgatctg tcgatcgatg atgcgaccaa
     5881 gggcatcgcc gggcacctcg acggcagcgg agcgccgctg cagggcaagc tgggctggca
     5941 acccgacatc tccagcaacc tgtctcgctt cggcgttcgc ggcgggcacg atctgggcga
     6001 aggcggatta aaggcggtgt tccagatcga aacccaggtc gacgtctccg ctacgccggg
     6061 caccgcttca tcgaattcct cctccgacaa caacgtcaag ggcgcctttg cctcgcgcaa
     6121 cagcttcctc ggaatggccg gcgacttcgg cgcagtcaag ctgggcaaga ccgacgcgcc
     6181 gtacaagctg tcgaccgggc gcatggatcc gttctcggcg acggtgggcg actacaacag
     6241 cattatgggt aacaccggcg gcgacaaccg cgccgagttc gacacccggc tgtcgcacgc
     6301 cgcgtggtac gagtcgccca agttcggcgc tttctcgttc agcgcgctgt tcgcccccgg
     6361 gcagaaccgc tcga
//
LOCUS       DATDMW010000030        20076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1376471, whole genome shotgun sequence.
ACCESSION   DATDMW010000030 DATDMW010000000
VERSION     DATDMW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376471"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1218)
                     /gene="mraY"
                     /locus_tag="VI319_00960"
     CDS             complement(64..1218)
                     /gene="mraY"
                     /locus_tag="VI319_00960"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY6862440.1"
                     /translation="MLLAMAQWLAQDVRAFNVFGYITLRAVLAAMTAMAISLIAGPMV
                     IRKLTAYKIGQMVRDDGPKSHLTKAGTPTMGGALVLLSIVITTLLWADLTNRFVWVVM
                     LVTFGFGAVGWVDDYRKVVQRNPKGLSARAKFFWSALIGLAAAVYLAFAVSAPSNTQV
                     LDLFMAWVRSGFEMNLPPKADLIVPFFKNIAYPLGVWGFITLTFFVIVGTSHAVNLTD
                     GLDGLAIMPAVMVAGALGIFAFVMGRVDFSRYLLIPYIPGAGELAVFCAALAGAGLGF
                     LWFNAYPAEVFMGDVGALALGGALGTVAVIVRQEIVLLIMGGVFVAETLSVMIQVLYF
                     KYSGGKRIFRMAPLHHHYELSGWKESQVVVRFWIITMMLVLFGLSTLKLR"
     gene            complement(1209..2600)
                     /gene="murF"
                     /locus_tag="VI319_00965"
     CDS             complement(1209..2600)
                     /gene="murF"
                     /locus_tag="VI319_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY6862441.1"
                     /translation="MMKVSEAAAAIGATTRGADTTFDSVGSDSRSLRGGELFVALRGE
                     RFDGHEFVATARQHGAVAAMVAEDAAESAAQSGLPLIVVHDTRLALGRLSSHWRGRFD
                     IPLVAVTGSNGKTTVKEMIASVLRAEHGEQRTLATEGNLNNDIGLPLTLLKLRSGHRC
                     AVIELGMNHPGETAYLAALASPTVALVNNAQREHQEFMKSIADVASEHGAVFAELRAG
                     GVAVINADDDFAAYWRSAVVGRPAARGPVRIVDFGLDHPAQVTGRYAFTGFGSEIALS
                     TPAGPASIRLQVPGRHNVLNAMAAASAAIASGARVDAVARGLEAFRAVKGRMQRKPGR
                     GGAVLIDDTYNANPDSVRAAIDVLTAQAGRPVLVLGDMGEVGEQGEAFHDEIGRYAKA
                     KGVVRLLATGPLSACAAQAFGEGASHFAQFDALVAAAVGEAGAGAVILVKGSRFMRME
                     RAVAALVEEAACC"
     gene            complement(2597..4084)
                     /locus_tag="VI319_00970"
     CDS             complement(2597..4084)
                     /locus_tag="VI319_00970"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY6862442.1"
                     /translation="MSDTVESILRRLAAQGLRVTGLSTDSRAIAPGETFLAYPGERSD
                     GRRFIGAALARGAAAVVWEREGFDWSGEWRVPNVPVEGLRALAGPLAHEVYGRPSEKL
                     WIIGVTGTNGKTSCTQWIARACGECGARTAVVGTLGTGFPGALEPGVNTTPDPVLLHR
                     SLARLLASGAQGAAIEVTSIGLDQGRLNGIAFGAAVFTNLSRDHLDYHGDMESYALAK
                     RRLFEVPGLRHAVLNLDDVQGVQIARMLAGGRVSRTGYSCFEGAAERAGLERYVEARA
                     ISLSADGLAFDLRSSWGDARVRSRLLGRFNVSNLLGAAGAMLVSGVPFECTVSALARM
                     EPVAGRMQRLGGGPAPLVVVDYAHSPDALEKTLATLKDVARAGSGRLIVVFGCGGERD
                     RGKRPLMGAVAARNADHVVVTSDNPRGEEPGAIIADIVAGIPGAHEVIEDRRAAIERA
                     VGMASAGDIVLLAGKGHEPYQEVAGRRLPFSDADEAERALARRTT"
     gene            complement(4087..5814)
                     /locus_tag="VI319_00975"
     CDS             complement(4087..5814)
                     /locus_tag="VI319_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY6862443.1"
                     /translation="MNFAPSPVLTLTLPPWRSRLLLAVFAGGFAFLIGRAVYLQGLHN
                     DFLQAKGESRYSRVLEIPANRGRISDRNGEPIAISTPVKSIWAAPDEVHMDKQQVARL
                     ASLLELSPREIQKRLKDARGEFVFLKRQVSPETAARVAELRIPGLSESREYRRYYPLG
                     DVMAHVLGFTGADDVGQEGVELAFQEQLAGKPGSRRVIKDRLGQIVEDVESIRAAQNG
                     RDLALALDSRIQNVAFTQLKSAVLRHRARAGAIVVMDVNTGEILALANVPTYNPNNRS
                     RLSGAQLRNRAVTDTFEPGSTLKPFTIALALESGRVRPDTVIATGSGSLTIGPATIHD
                     AHAERALTVAQVIQKSSNVGAAKIALSLTAQSMWEMFEHVGFGAPPGLDFPGEASGKL
                     RPYRTWRTIEQATMSYGHGISVSLVQLIRAYSIFARDGELVPLSLLKVDAPPVVRRVV
                     SAQTARMVRAMLELAVQRGGTAPRAQIAGYRVAGKTGTAHKQEDGHYATDKYVSSFVG
                     FAPASRPRLAIAVMIDEPTGEYYGGKVAAPVFAEVMASALRILDVAPDAPIKPIELPD
                     ESDEIKESV"
     gene            complement(5811..6086)
                     /gene="ftsL"
                     /locus_tag="VI319_00980"
     CDS             complement(5811..6086)
                     /gene="ftsL"
                     /locus_tag="VI319_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEY6862444.1"
                     /translation="MLRVTLILLAVLVACALGLITSQHKARKLFAELETEQERTRALD
                     VEYGQLQLEASTWSTQSRIERIASQRLRMRHPAPSGVQMVEPPKNEP"
     gene            complement(6112..7041)
                     /gene="rsmH"
                     /locus_tag="VI319_00985"
     CDS             complement(6112..7041)
                     /gene="rsmH"
                     /locus_tag="VI319_00985"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY6862445.1"
                     /translation="MTADAHVPVLAGEAVEALAVARRPDGVFVDATFGRGGHSRLILE
                     RLGRVGRLIAIDRDPRAITAAAGLRDSRLTAVHARFAALDSILRAQGVAAIDGALFDL
                     GVSSPQLDDPDRGFSFRFDAPLDMRMDPTSGNSAADWLARATEGDIREVLSTHGEERF
                     AKQIAKAIVAARRAGPVRRTGQLAALVGQAVRTREPGQDPATRTFQALRIHVNQELEE
                     LSLALPRALDALGGGGRLAVISFHSLEDRIVKNFMREAARPSAPERLPLRARELPAPR
                     LRVIGKPIRPAPAEIAANPRARSAVLRVAEKLA"
     gene            complement(7038..7481)
                     /gene="mraZ"
                     /locus_tag="VI319_00990"
     CDS             complement(7038..7481)
                     /gene="mraZ"
                     /locus_tag="VI319_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY6862446.1"
                     /translation="MFQGATELTLDAKGRLAVPTRHRDALLSGGRGLVLTAHPDGCLL
                     LYPGLAWEPIGARLQALSSFNEQARWWQRLLVGFAEETEPDAQGRVLISPALRKFAHL
                     EKEIMLVGQGSHFELWSAQAWDERLARALGQVGANPPPGTENFSL"
     gene            complement(7824..8372)
                     /locus_tag="VI319_00995"
     CDS             complement(7824..8372)
                     /locus_tag="VI319_00995"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HEY6862447.1"
                     /translation="MHTEVHVHGTVSLRNGVTQPEVEQALRPWLDYVDIDGLADARSA
                     VQDEPGIVFDRRRRALEICWTGYVGRNFQNSLDAAFQLLGPYSEEAVAVEVSYYHEDG
                     QDEHGWVFVGPTHEAIHEMQRRRLVDDVAMLLARHFGDAEIGEVSALLNQLFARKASS
                     PATGAEREQSRTMRVPGKRHLH"
     gene            8523..9341
                     /locus_tag="VI319_01000"
     CDS             8523..9341
                     /locus_tag="VI319_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY6862448.1"
                     /translation="MANPIDTINMALFCDFENVALGVRDAKYAKFDISKVLERLLLKG
                     SIVVKKAYCDWERYKEFKADMHQASFELIEIPHVRMSGKNSADIRMVVDALDLCYTKS
                     HVDAFVIISGDSDFSPLVSKLRENDKTVIGVGVKNSTSDLLIANCDEFIFYDDLVRQK
                     PARRPAKKKQTEKAPQQSADASEAPKDGDDKKEKAIEMVTDTLDALVAERGPEEKIWG
                     SMVKQALKRRNPGFNESYYGFSSFSKLLEEAQARKLLELEHDEKSGGYIIRATH"
     gene            complement(9352..10557)
                     /gene="pcaF"
                     /locus_tag="VI319_01005"
     CDS             complement(9352..10557)
                     /gene="pcaF"
                     /locus_tag="VI319_01005"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEY6862449.1"
                     /translation="MPEAFICDAVRTPFGRYGGALSPIRTDDLAALPLKALMERNAAL
                     DWAALDDVVYGCVNQAGEDNRNVARMALLLAGLPQGVPGATVNRLCGSSMDALGTAAR
                     AIRAGETELMIAGGVESMSRAPFVLPKAESAFSRTSAIEDTTIGWRFVNPVMKAKYGI
                     DSMPETAENVAAEFKVARADQDAFALRSQQRWAASQAAGFYRAEIVPVQIPGKKGAVT
                     TVDRDEHPRPDTTLEALAKLRGIVKPEGTVTAGNASGVNDGACAMLLASEAAAKKHRL
                     EPRARVLAMATAGVAPRIMGIGPTPASKKVLEKTGLKISKMDVIELNEAFAAQALAVT
                     RALGLPDDAPHVNPNGGAIAIGHPLGASGARLVTTAVAQLRATKGRYALCTMCIGVGQ
                     GIAVIVERV"
     gene            complement(10615..11316)
                     /locus_tag="VI319_01010"
     CDS             complement(10615..11316)
                     /locus_tag="VI319_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6862450.1"
                     /translation="MLAGRTALITGAGGNLGRAAAEAFGAAGAALVLVGRTEASLRPE
                     YGAGDARTMLLGADLSDAGAVAVAVGQALARFPRIDIVCNIAGGFDMGPPVHQTPDEL
                     WQRMVDLNVRTMLNTVRAVVPGMRAARYGKIVNVGAMAGLAGKAHMGAYAAAKSMVIR
                     LTESMSAELRDDGINVNCVLPSIIDTPQNRAAMPKADPRRWVSPAALADVLLFLVSDK
                     SAPIHGAAIPVTGLV"
     gene            complement(11331..12293)
                     /locus_tag="VI319_01015"
     CDS             complement(11331..12293)
                     /locus_tag="VI319_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862451.1"
                     /translation="MKMLKCVIAAVTAALAGHASAQGFPNRPVHVIVSFTAGSSTDIV
                     GRVVTQKLAEIWGQPVVVENRAGAGGSIGSAYVVKQPPDGYTLLVDSNAHTVTPSIYA
                     KLPYDVSKDFTDIAPFALQPNVLVVTVDSKYKTLMDIVNDAKSRPGAINWGHAGVGSG
                     THLNTEKFIAAANIKVTEIPFKGTPEVVSSMLSGSVDCYWAPISAALSLIKGGKLRPL
                     AVSTAKRNSTLPDVPTTGEAGVQGADAPLWFALWGPAGMAPGLVNKISTDTRRALKEK
                     DVRDRLTQLGNDTMDMSPAEFKKFVASEVQEYARVVKNAGIKPQ"
     gene            complement(12330..13295)
                     /locus_tag="VI319_01020"
     CDS             complement(12330..13295)
                     /locus_tag="VI319_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862452.1"
                     /translation="MKASRAALAVVAALAVAGAVHAQSYPSGPVKVIVPFTAGSATDI
                     MARTVGEKLSSALGKPVIVENHPGAGGTIGINLVAKAAPDGLTLGVVSTGHTVNHVLY
                     KDLPYDTIRDFAGVSPLATLPSVLIVAPSLGVKTVRDLVALAKSKPGQLNYGTAGVGS
                     AAHINQEKFNQASGIQAVHIPFKGTPEILNEAMAGRIEMAWVPLISSVGPIRDGKVVA
                     LAVSTPNRSETLPQVPTIKEAGYPGGEFNFWVGMLAPAKTPRAVVTRLNAEIAKAEQA
                     PEVRARIAKLGAEPMPMSPEKFDAFIKEQYEDLGKIMRAAGAKPQ"
     gene            complement(13318..13977)
                     /locus_tag="VI319_01025"
     CDS             complement(13318..13977)
                     /locus_tag="VI319_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862453.1"
                     /translation="MAKQRISYVALDKMDAQMQKEMDRCRREGTPRPESSAVRAHVPA
                     CFWFFANSWRDIFRNGVLDHAIKELCRLYVSRSVECEYCGNQRSVRATTSGAVIEDHV
                     KDLLNFEKSTKYDDRQKAALAYAEAIVWRTETDDAFWERMKRHFSEPELVELGCMIGL
                     TLGQQSWLRLLNIEHHQVLADTSASMAPGYETMDALKKTKSADDYWAGKSKKKKTETA
                     A"
     gene            complement(13996..15222)
                     /locus_tag="VI319_01030"
     CDS             complement(13996..15222)
                     /locus_tag="VI319_01030"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY6862454.1"
                     /translation="MSAPVLDCRRLPPADAARAIVERFNALEPGARFEALVADYGPPL
                     RVSLLEAGIRHAAELTAERGRRLAIRRGAVPAQGTIPGVHHVVTDGDSVWTCERGERV
                     ARIGVGGEPVAVARVARKASHLALEHRSGTLFIADAQGNEVIALHAADLRVIARWRAP
                     GGPQLPLAAPDGVVCVTGGGTGTLTIATPRRGGYSERTVEVGTCPHDPLLDPDAAHVL
                     VPCAGDGELVKVRLADGRIVGRTRVGEGPSHLARRADGRVYCANSWDGTVACVGTEGE
                     IVAAAPSGGWAHAIDVSPDGTRVFVANFLDDTVCVFDAETLERVALIETGPYPHGLDV
                     SPDGRHVVVTAFGGAEIQVLDARTPRELGRVAVGRGSSHTAFCAGMAWVGCSVDDHIA
                     CVDLAKLSATRQISIH"
     gene            complement(15219..16265)
                     /locus_tag="VI319_01035"
     CDS             complement(15219..16265)
                     /locus_tag="VI319_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY6862455.1"
                     /translation="MGDKYPVVKVAAVQAASVFLDREGSTEKACRLIREAGRNGARVI
                     GFPEGFIPAHPVWYHHHAATGAVANELAAALFRNSVEIPGPEIDALCAAARDAKAYVV
                     VGVCEKLPRTIGTMFNTQVYIGPDGTLLGKHQKLVPTVGERLVHAGGFGDTFGAFQTE
                     FGPMSGLICGENSNPLAVFALTAEGTRIHVMSWPNHFPTSGDPMRNRVLVDSQAFAQM
                     SKAFVVSACGTVDEAMIGMLKVGPEAEKFLRSPDCCGGSAIVAPNSRVLAGPMGPEEG
                     ILYAECDIAIGVQAKLRHDFAGHYNRPDVFQLHINRAAPRLYTVHGESETPALGEAGQ
                     PRLPAGFEKVELKK"
     gene            complement(16357..16767)
                     /locus_tag="VI319_01040"
     CDS             complement(16357..16767)
                     /locus_tag="VI319_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006295980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEY6862456.1"
                     /translation="MAGYSGTPLAGKLGLKPPMTLVALNAPLEYPSWLGKLAPGIDLA
                     SKASAPLRAVHLFATNRGALEKQLASLRKRLEQTGFVWVSWPKKASKVPTDITEDVIR
                     AVALPLGFVDVKVCAVSEVWSGLKLVIRKSERTS"
     gene            complement(16774..17613)
                     /locus_tag="VI319_01045"
     CDS             complement(16774..17613)
                     /locus_tag="VI319_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6862457.1"
                     /translation="MRKNAWLLLAALLWAQVARPQTTDVLIEVNTGRTEGVFTKSPVL
                     ERAILAMPARPTDTALLYFRGYPGIARIQSAADKQRNLQPFMRKNQQILAEEGIALVV
                     VDCPTDQWGGNSPKPTACFDGYRSSQQHADDVRKIIAKLKSEHGFTRIYIMGHSMGSV
                     SSRWLAKNLGTEISGSIHSASMNRRTGDGFAGSVSGFPYGEIKAPALHVHNENDACPY
                     TPYSAVKAYAGENLVTVRGGTPHGDPCGGGHFHSFEGREEAVVRAIVAWIKTGKVERF
                     VGE"
     gene            complement(17648..18046)
                     /locus_tag="VI319_01050"
     CDS             complement(17648..18046)
                     /locus_tag="VI319_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862458.1"
                     /translation="MRLFLAIVFALASAAAGAECVPYESEATLKGIVHFRVAPGAPNY
                     ENIEAGDRAERQPILDLYEPTCIEGQRGGITITRQVLSVQLLPPPGSRLASLENRPVT
                     VIGTISGRMTVNAKVPVLMRMTSVRADPRR"
     gene            complement(18060..19007)
                     /locus_tag="VI319_01055"
     CDS             complement(18060..19007)
                     /locus_tag="VI319_01055"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6862459.1"
                     /translation="MAERKPKIGWIGTGRMGFEMAQRLAQAGCDISAWNRTRAKAEPL
                     AKDGAKIAADLPSLAGCDIVFVMVSTWNDVKEVVSGPAGLLSKSQPGKGPAPRIVVEC
                     SSISLEGSAELRQILKPYGTEYVASPVSGNAKVIKAGKLTFVCSGPKKAYEEVAPLLA
                     RIAPLGGSYVGEGELSRIVKICHNVMLGVVTQCMAEITVLAEKAGVPRHAFLEFLNKS
                     VMGSTFTRYKAPAFVNLDFHVTFTPYLLRKDLDLGLEAGRKFEVPMPLASITRDLIQS
                     MMGRGMTEEDFSKLLVQQAQASGLALQPENVKVGDGLSS"
     gene            complement(19033..>20076)
                     /locus_tag="VI319_01060"
     CDS             complement(19033..>20076)
                     /locus_tag="VI319_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY6862460.1"
                     /translation="DKLTRYSLLTGNGDPYIWDFGSAAKHHRLYAPWLHVDHCKAGLL
                     GLRGATGGDITLFREAAKEIKGILDAEGVGDMPLGLDVVEPPMLFELQKLGIEVRDGQ
                     QVMLAAREVKNIDELTLLNMASAMVDGVYQDIAEALKPGVQESQIVALATKRLYEMGS
                     DCVEAINSISGERCSPHPHNFTDRLIRPGDQAFFDIIQSFMGYRTCYYRTFNVGKATP
                     AQRTAYRKAREWMDKAIELLKPGMTSDRIARALPEAKDIGFSSEMEAFGLNFCHGLGL
                     GLHERPLISRLTSLKEPIELKAGMVFAVETYCPATDGYSAARIEEEVILTPQGAKIIS
                     LYPADELPIANPY"
BASE COUNT         3060 a         7013 c         6779 g         3224 t
ORIGIN      
        1 tcgaatcatt tttcttgtct tgttccctgt gcctttgcgc tcttgcgccc tgcgccttgc
       61 gccttatctc aacttcaacg tcgacagccc gaacagcacc agcatcatcg tgatgatcca
      121 gaagcgcacc acgacctgcg actccttcca tccggagagc tcgtaatggt ggtgcagggg
      181 cgccatgcgg aagattcgct tgccgcccga gtacttgaag tacagcacct ggatcatcac
      241 cgagagcgtc tcggcgacga acactccgcc catgatcagg agcacgatct cctgccgtac
      301 gatcaccgcc accgtgccga gcgcgccgcc gagcgcaagc gcgcccacgt cacccatgaa
      361 cacctcggcc gggtacgcgt tgaaccacag gaagccgagg ccggcgcccg cgagcgcggc
      421 gcagaacacc gccagctcgc cagcgcccgg gatgtacggg atgaggagat agcgcgagaa
      481 gtcgacgcgg cccatcacga aggcgaagat gccgagcgcg ccggcgacca tcaccgccgg
      541 catgatcgcg aggccgtcca ggccgtcggt cagattgacg gcatgactcg tgccgacgat
      601 cacgaagaag gtgagcgtga tgaagcccca gacgccgagc gggtacgcga tgttcttgaa
      661 gaacggcacg atgaggtccg ccttcggcgg aaggttcatc tcgaagccgc tgcgcaccca
      721 cgccatgaaa aggtcgagca cctgagtgtt cgacggcgca gagaccgcga acgcgagata
      781 gacggcggcg gcgagcccga tgagcgccga ccagaagaac ttggctctgg ccgacagccc
      841 cttcgggttg cgctgcacga ccttgcgata gtcgtccacc cagccgacgg cgccgaaacc
      901 gaaggtgacc agcatgacga cccagacgaa gcggttggtg agatcggccc agagcagcgt
      961 cgtgatgacg atcgacagca gcaccagcgc cccgcccatg gtcggggtgc ccgccttggt
     1021 gagatgggac ttgggcccgt cgtcgcgcac catctggccg atcttgtagg ccgtgagctt
     1081 gcgaatgacc atcggaccgg cgatcagcga gatggccatg gcggtcatcg ccgccagcac
     1141 cgcgcgcagc gtgatgtagc cgaagacgtt gaacgcgcgg acgtcctgcg cgagccactg
     1201 agccattgct agcagcatgc cgcctcctcg accagcgccg cgaccgcgcg ctccatgcgc
     1261 atgaagcgcg agcccttcac caggatcacc gcaccggcac cggcctcgcc caccgcggcg
     1321 gcgaccagcg cgtcgaactg cgcgaaatgc gacgcccctt cgccgaaggc ttgcgccgcg
     1381 cacgcgctga gcggccccgt cgcgagcagc cgcacgacac ccttggcctt cgcgtagcgc
     1441 ccgatctcgt cgtggaacgc ctcgccctgc tcgccgacct cgcccatgtc gccgagaacg
     1501 agcaccgggc ggcccgcctg cgcggtcagc acgtcgatcg cggcccgcac ggaatccggg
     1561 ttggcgttgt aggtgtcgtc gatgagcacc gcgccgccgc gtccgggctt gcgctgcatg
     1621 cgccccttga cggcgcggaa cgcctcgagg ccgcgcgcga cggcgtcgac gcgggcgccc
     1681 gatgcgatcg ccgcgctcgc ggccgccatc gcgttcagca cgttgtggcg tcccgggacc
     1741 tgcagccgta tcgaagcggg ccccgccggc gtgctgagcg cgatctcgct gccgaagccg
     1801 gtgaacgcat accgccccgt cacctgcgcg ggatgatcga ggccgaagtc gacgatgcgc
     1861 accggtcctc tcgcggcggg cctgcccacg accgcactgc gccagtaggc cgcgaagtcg
     1921 tcgtccgcgt tgatcaccgc gacgcctccc gcgcgcagct cggcgaacac cgcgccgtgc
     1981 tcgctcgcca cgtcggcgat gctcttcatg aattcctggt gctcgcgctg cgcgttgttc
     2041 acgagggcga ccgtcgggga ggccagagcg gcgaggtacg ccgtctcgcc cggatgattc
     2101 attcccagct cgatcaccgc gcaacggtgt cccgagcgca gcttgagcag cgtgagcggc
     2161 aggccgatgt cgttgttgag gttgccctcg gtcgcgagcg tgcgctgctc gccgtgctcg
     2221 gcgcgcagaa ccgaggcgat catctccttg accgtggtct tgccgttcga gccggtcacc
     2281 gccacgagcg ggatgtcgaa gcgcccgcgc cagtgcgacg acaggcggcc gagcgcgagg
     2341 cgcgtgtcgt gcacgacaat gagcggcagc cccgattgcg cggccgactc ggccgcgtcc
     2401 tcggccacca tcgccgcgac ggcgccatgc tgccgagcgg tggccacgaa ctcgtgtccg
     2461 tcgaagcgct cgccgcgcag cgcgacgaac agctcgccgc cgcgaaggct gcggctgtcg
     2521 ctgcccaccg agtcgaaggt cgtgtcggcg ccgcgcgtcg tcgcgccgat cgccgccgcc
     2581 gcttcgctga ccttcatcat gtcgttcgcc tcgcgagcgc gcgctcggct tcgtccgcgt
     2641 cggagaacgg caggcgacgc ccggccacct cctggtacgg ctcgtgtccc ttgccggcga
     2701 gcagcacgat gtcgcccgcg ctcgccatgc cgaccgcgcg ctcgatcgcg gcgcggcggt
     2761 cctcgatcac ctcgtgagcg ccgggtatcc cggcgacgat gtcggcgatg atggcgccgg
     2821 gctcctcccc acgcggattg tcgctggtca cgacgacatg atcggcgttg cgcgccgcga
     2881 ccgcgcccat cagcgggcgc ttcccccggt cgcgctcgcc gccgcagccg aacacgacga
     2941 tcaaccgccc gctccccgcg cgggcgacgt ccttgagcgt tgcgagcgtc ttctcgagcg
     3001 cgtcgggact gtgggcgtag tcgacgacga cgagcggcgc cgggccgccg cccaggcgct
     3061 gcatgcgccc ggcgaccggt tccatgcgcg ccagcgcgga cacggtgcac tcgaacggca
     3121 cgccggagac cagcatggcg ccagccgcgc cgagcaggtt cgatacgttg aaacggccga
     3181 gcagccggct ccgcacgcgc gcgtcgcccc aggagctgcg cagatcgaac gcgaggccgt
     3241 ccgcggagag cgagatcgcg cgcgcctcga cgtagcgctc gagacccgcg cgctcggccg
     3301 cgccctcgaa gcagctgtag cctgtgcggc tgacgcgccc gccggcgagc atgcgggcga
     3361 tctgcacgcc ctgcacgtcg tcgagattca gcaccgcgtg gcgcaatccg ggcacctcga
     3421 acagccgccg cttcgcgagc gcgtagctct ccatgtcgcc gtggtagtcg agatggtcgc
     3481 gcgacagatt ggtaaagacc gccgcgccga atgcgatgcc gttcagccgc ccctggtcga
     3541 ggccgatcga ggtgacctcg atcgccgcgc cctgggcgcc ggatgcgagc agccgcgcga
     3601 gcgatcgatg cagcagcacc gggtccggcg tggtgttgac gccaggctcg agcgctccgg
     3661 gaaatccggt gccgagcgtc ccgaccaccg cggtccgcgc gccgcactcg ccgcaggcgc
     3721 gcgcgatcca ctgcgtgcag gaggtcttgc cgttggtacc ggtcacgccg atgatccaca
     3781 gcttctccga cgggcgtccg tagacctcgt gcgcgagcgg cccggcgagc gcgcgcaagc
     3841 cctccaccgg aacgttcggt acgcgccact cgccgctcca gtcgaagccc tcgcgctccc
     3901 agacgacggc cgcggccccg cgcgcgagcg cggcgccgat gaagcgccga ccgtcgctgc
     3961 gctcgccggg ataggcgagg aacgtctcgc ccggcgcgat ggcgcggctg tcggtcgaca
     4021 ggccggtcac gcgcagacct tgcgcggcga gccgccgcag gatcgattcc accgtgtcgg
     4081 acacacctat acgctttcct tgatttcgtc ggactcgtcg ggaagctcga tcggcttgat
     4141 cggcgcgtcg ggcgcgacgt cgaggatccg cagcgccgac gccatgacct ccgcgaacac
     4201 cggtgccgcc accttgccgc cgtaatactc gccggtcggc tcgtcgatca tcaccgcgat
     4261 cgccagtcgc ggacgcgaag cgggcgcgaa gccgacgaac gaagagacat acttgtccgt
     4321 ggcatagtgg ccgtcctcct gcttgtgcgc ggtgccggtc ttgcccgcga cccggtagcc
     4381 cgcgatctgc gcgcgcggcg ccgtgccgcc gcgctgcacc gcgagctcga gcatggcgcg
     4441 caccatgcgc gcggtctgcg cggacacgac gcggcgaacg accggcggcg catcgacttt
     4501 gagcagcgag agcggcacca gctcgccgtc gcgcgcgaag atgctgtagg cgcggatcag
     4561 ctgcaccagg gacaccgaga tcccgtgccc gtacgacatg gtcgcctgct cgatcgttcg
     4621 ccaggtgcgg tacgggcgca gctttcccga cgcctctccc gggaaatcca ggcccggcgg
     4681 cgcgccgaac ccgacgtgct cgaacatttc ccacatcgac tgcgcggtca gcgacagcgc
     4741 gatcttggcc gcgccgacgt tggaggactt ctggatgacc tgcgcgacgg tgagcgctcg
     4801 ctcggcgtgc gcgtcgtgga tcgtggccgg accgatcgtc aggctgccgg agccggtcgc
     4861 gatgacggtg tcggggcgca cgcgaccgct ctccagggcg agcgcgatgg tgaagggctt
     4921 gagcgtggaa ccgggctcga acgtatcggt gaccgcgcgg ttgcgcagct gcgcgcccga
     4981 gagccggctg cggttgttcg ggttgtaggt cgggacgttt gcgagcgcga ggatctcgcc
     5041 ggtgttcacg tccatcacga cgatggcgcc ggcgcgcgca cggtgacgca gtaccgccga
     5101 tttgagctgc gtgaacgcga cgttctgaat gcgcgagtcg agcgcgagcg ccagatcgcg
     5161 gccgttctgc gccgcgcgta tcgattccac gtcctcgacg atctgcccga gccggtcctt
     5221 gatcacgcgg cggctgcccg gctttccggc gagctgctcc tggaacgcga gctcgacgcc
     5281 ctcctggccg acgtcgtcgg cgccggtgaa cccgagcacg tgcgccatca catcaccgag
     5341 cggatagtag cggcggtact cgcggctctc ggagagcccc gggatccgga gctcggcgac
     5401 gcgcgcggcg gtctcgggcg atacctggcg cttcaggaac acgaactcgc cgcgtgcatc
     5461 cttcaggcgc ttctggatct cgcgcgggga cagctcgagc agcgacgcca ggcgcgcgac
     5521 ctgctgcttg tccatgtgga cctcgtcggg cgcggcccag atcgatttca cgggcgtgct
     5581 gatcgcgatc ggctcgccgt tgcggtcgct gatgcgcccg cggttcgccg ggatctcgag
     5641 cacccggctg tagcgcgatt cgcctttcgc ctgcaggaag tcgttgtgca ggccctgcag
     5701 gtataccgcc ctcccgatca gaaaggcgaa gccgccggcg aacacggcca gcagcagccg
     5761 cgagcgccac ggcggcagcg tcagcgtcag caccgggctg ggcgcgaagt tcacggctcg
     5821 ttcttcgggg gctcgaccat ctggacgccg ctgggcgcgg ggtgacgcat gcgcagccgc
     5881 tggctcgcga tgcgctcgat gcgcgactgc gtcgaccagg tgctcgcctc gagctgcagc
     5941 tgcccgtact ccacgtcgag cgcgcgcgtg cgctcctgct cggtctcgag ctcggcgaaa
     6001 agtttgcgcg ccttgtgctg cgacgtgatc aggccaagcg cgcacgcgac cagcaccgcg
     6061 aggaggatca gcgtgacgcg cagcatcggc ggctcgtctc ggcgccccgg ctcaggcgag
     6121 cttctcggcg acgcgcagca cggcgctgcg cgcgcggggg ttggcggcga tctcggcggg
     6181 cgccggccgg atcggcttgc cgatcacgcg caagcgcggc gccggcagct cgcgtgcgcg
     6241 cagcggcagg cgttcgggcg cggacggccg cgccgcctcg cgcatgaagt tcttgacgat
     6301 gcggtcctcg agagaatgga agctgatcac cgcgaggcgc ccgcccccgc cgagcgcatc
     6361 gagcgcgcgc ggcagtgcta acgacaattc ctcaagctcc tgattgacgt gaatccgtag
     6421 agcttgaaac gtgcgcgtcg ccgggtcctg ccccggctca cgcgtccgga cggcctgacc
     6481 cacgagagcg gcaagctgcc cggtcctgcg gacaggccct gctcgccgag cagcaacaat
     6541 cgcctttgca atctgtttag caaaccgttc ttccccatga gtgctcaaca cctccctgat
     6601 gtcgccctcg gtagcgcgcg ccagccagtc ggcggcgctg tttccgctcg tcggatccat
     6661 gcgcatgtcg agcggtgcgt cgaaacggaa gctgaagcct cgatccgggt cgtcgagctg
     6721 gggcgaggac accccgaggt cgaagagcgc gccgtcgatc gccgccacgc cctgcgcgcg
     6781 gaggatcgag tcgagcgctg caaagcgggc gtgcaccgcg gtcaggcgcg agtcgcgcag
     6841 cccggctgcc gccgtaatgg cccgcggatc gcggtcgatg gcgatcaggc ggccgacgcg
     6901 gccgagccgc tcgaggatga ggcggctgtg cccgccccgg ccgaaggtcg catccacgaa
     6961 gacgccgtcc ggacggcgcg caaccgcgag cgcctcgacg gcctcgcccg cgagcaccgg
     7021 gacgtgcgca tcggcggtca cagcgagaaa ttttcggtgc ccggcggcgg gttggcgccc
     7081 acctggccca gggcgcgcgc gaggcgctcg tcccacgcct gcgcgctcca gagctcgaaa
     7141 tggctcccct gtcccacgag catgatctcc ttctcgagat gcgcgaactt gcgcagcgcc
     7201 ggcgagatga gcacccggcc ctgcgcgtcg ggctcggtct cctcggcgaa gccgacgagc
     7261 agccgctgcc accagcgcgc ctgctcgttg aagctcgaca acgcctgcag cctggcgccg
     7321 atcggttccc acgcgagccc ggggtagagc agaaggcaac cgtccgggtg cgcagtgagg
     7381 acgagcccgc gcccgccgga gagcagcgcg tcccggtgcc gcgtcggcac cgcgagccgc
     7441 cccttggcgt cgagagtgag ctcggtcgcc ccttggaaca cccgtcctcc tgtgtgtggc
     7501 ggaaagcttg ctgtgtaaca ccacagtttc ccaccaaatt ctacttatta ccactttaga
     7561 ggaaaggatg gcgccgagtc aaggcgcgtt tgtgaaaatt tgcagctaca acagatactt
     7621 aagaagggtt cctcagaaca tttctgaact tgaaatggtc ttaagaatta actgcttatc
     7681 cgcgaaacct catgtaaagc cttaagaaaa tcaacagctt gagattacgc gggaattccg
     7741 tgcgaaggcg gtcggacctg tggccggccg agggcgcatt ctgccctggc gatgacgcgt
     7801 ggacgtgcgg agttagtggg tactcagtgc aggtgccgct tgcctgggac gcgcatggtt
     7861 ctcgactgct cgcgctcggc cccggtcgcc gggctgctcg ccttgcgggc aaagagctgg
     7921 ttgagcagcg ccgacacctc accgatctcg gcgtcgccga aatgacgcgc gagcagcatc
     7981 gcgacgtcgt cgaccagccg acgacgctgc atctcgtgga tcgcctcgtg cgtcgggccg
     8041 acgaacaccc agccgtgctc gtcctgaccg tcctcgtggt aatagctgac ctcgacggcg
     8101 acggcctcct cggagtaggg tccgagcagc tggaaggccg catcgagcga gttttggaaa
     8161 ttgcggccga cgtagcccgt ccagcagatc tcgagcgccc ggcgacggcg gtcgaatacg
     8221 atgccgggct cgtcctggac cgcgctgcgc gcgtcggcca gcccgtcgat gtcgacgtaa
     8281 tccagccacg gccgcagggc ctgctcgacc tcgggctggg tgacgccgtt ccggagcgaa
     8341 acggtgccgt ggacgtggac ctcggtatgc attcgggggg actccttcgg aagggtcgat
     8401 gctattccga ccgccgcgga acttcaaccc tggccggcac gcggaagttg tgcggggcat
     8461 ccccatccga cataatggcc cgcgccgcgg cgtccgcgcg gacatcctcc cattccccac
     8521 ccatggcgaa tcccatcgac accatcaaca tggcgctgtt ctgcgacttc gagaacgtcg
     8581 cgctcggcgt gcgcgacgcg aagtacgcga agttcgacat cagcaaggtg ctcgagcgcc
     8641 tcctgctcaa gggcagcatc gtggtcaaga aggcctactg cgactgggag cgctacaagg
     8701 aattcaaggc cgacatgcac caggcctcgt tcgagctgat cgagatcccg cacgtgcgca
     8761 tgtcgggcaa gaactcggcc gacatccgca tggtcgtcga cgcgctcgac ctctgctaca
     8821 ccaagtcgca cgtcgacgcc ttcgtgatca tcagcgggga ttccgatttt tcgccgctcg
     8881 tctccaagct gcgcgagaac gacaagacgg tgatcggcgt gggcgtgaag aactcgacct
     8941 ccgatctgct gatcgccaac tgcgacgagt tcatcttcta cgacgacctc gtgcgccaga
     9001 agcccgcgcg ccgtcccgcg aagaaaaagc agaccgagaa ggcgccgcag cagtcggccg
     9061 acgcgtccga ggcgcccaag gacggcgacg acaagaagga gaaggcgatc gagatggtga
     9121 ccgacacgct cgacgcgctg gtcgccgagc gcggccccga ggagaagatc tggggctcga
     9181 tggtgaagca ggcgctcaag cgacgcaacc cgggattcaa cgagtcgtac tacggcttct
     9241 cgtcgttctc caagctgctc gaggaagcgc aggcgcgcaa gctcctcgag ctcgagcacg
     9301 acgagaagtc gggcggctac atcattcgcg ccacgcacta gcgcgggcga ctcacacccg
     9361 ctcgacgatc accgcgatgc cctgcccgac gccgatgcac atcgtgcaca gggcgtaacg
     9421 gcccttcgtc gcgcgcagct gcgcgacggc ggtcgtcaca aggcgcgcgc cggaagcgcc
     9481 gagcgggtgt ccgatcgcga tcgcgccgcc gttcggattg acgtgcggcg cgtcgtcggg
     9541 caacccgagc gcgcgcgtca ccgcgagcgc ctgcgcggcg aaggcttcgt tcagctcgat
     9601 cacgtccatc ttcgagatct tgaggccggt cttctcgagc accttcttcg acgcgggcgt
     9661 cgggccgatg cccatgatgc gcggcgcgac gcccgcggtc gccatcgcca ggacgcgagc
     9721 cctgggctcg agccggtgct tcttcgccgc cgcctcgctc gcgaggagca tggcgcaggc
     9781 gccgtcgttc acgcccgagg cgttgcccgc cgtgaccgtg ccttcgggct tcacgatgcc
     9841 tctcagcttc gcgagcgcct cgagcgtcgt gtccggacgc ggatgctcgt cgcgatccac
     9901 cgtcgtcacc gcgcctttct tccccgggat ctggaccgga acgatctcgg cgcggtagaa
     9961 gcccgcagcc tgcgacgccg cccagcgctg ctggctgcgc agcgcgaaag cgtcctggtc
    10021 ggcgcgcgcg accttgaact cggcggcgac gttctcggcc gtctcgggca tcgagtcgat
    10081 gccgtacttc gccttcatga ccgggttcac gaagcgccag ccgatggtcg tgtcctcgat
    10141 cgcggacgtg cgcgagaagg cgctctcggc tttcgggagc acgaacggcg cgcgcgacat
    10201 gctctcgacg ccgcccgcga tcatcagctc ggtctcgccc gcgcggatcg cgcgcgccgc
    10261 ggtcccgagc gcgtccatgc tcgagccgca cagacgattg acggtcgcgc cggggacgcc
    10321 ctgcggcagc ccggcgagga gcaacgccat gcgcgcgacg ttgcggttgt cctcgccggc
    10381 ctggttgacg cagccgtaga cgacgtcgtc gagcgccgcc cagtcgagcg cggcgttgcg
    10441 ctccatgagc gccttcagcg gcagcgccgc gaggtcgtcg gtgcggatcg gggatagcgc
    10501 gccgccgtaa cggccgaaag gcgtgcgcac ggcgtcgcag atgaaggctt cgggcatgtc
    10561 ggaggcgatg cgaaggatga ctcggaggcg ccaagcatag cgcccggcga agcttcagac
    10621 cagtcccgtg accgggatcg cggcgccgtg gatcggggcc gacttgtccg agacgaggaa
    10681 cagcaggacg tcggcgagcg ccgcgggcga gacccagcgc cgcggatcgg ccttcggcat
    10741 cgccgcccgg ttctgcggcg tgtcgatgat gctcggcagc acgcaattga cgttgatgcc
    10801 gtcgtcgcgc agctccgcgg acatgctttc ggtgagccgg atgaccatgc tcttcgcggc
    10861 ggcgtacgcg cccatgtgcg ccttgcctgc gagacccgcc atcgcgccga cgttgacgat
    10921 cttgccatag cgcgccgcgc gcatccccgg cacgacggcg cgcacggtat tgagcatcgt
    10981 gcgcacgttg aggtcgacca tgcgctgcca cagctcgtcc ggggtctggt gtaccggggg
    11041 tcccatgtcg aagccgcccg cgatgttgca gacgatgtcg atgcgcggga agcgcgcgag
    11101 cgcctgcccg accgcgaccg ccactgcccc ggcgtccgac agatcggctc cgaggagcat
    11161 cgtgcgcgcg tcgccggctc catactcggg ccgcagcgac gcttcggtgc ggccgacgag
    11221 cacgagcgcg gcgcccgcgg cgccgaacgc ctccgccgcc gcgcggccca gattgccgcc
    11281 ggcccccgtg atcagagcag tccggccggc aagcacgtta ccgcggcacc ttactgcggc
    11341 ttgatccccg cgttcttcac cacgcgggcg tactcctgga cttcgctcgc gacgaacttc
    11401 ttgaactccg cgggggacat gtccatggtg tcgttgccga gctgcgtcag gcggtcgcgc
    11461 acgtccttct ccttcagcgc cctgcgcgtg tccgtggaga tcttgtttac gagcccgggc
    11521 gccattcccg ccggtcccca gagcgcgaac cacagcggcg cgtccgcgcc ctggacacca
    11581 gcctcgccgg tggtcggcac gtcgggcagc gtgctgttgc gcttcgccgt gctcaccgcg
    11641 agcggccgca gcttgccgcc cttgatcagc gacagcgccg cggagatcgg cgcccagtag
    11701 cagtcgaccg agccgctcag catcgacgac accacctcgg gcgtaccctt gaatggaatc
    11761 tcggtgacct tgatattcgc cgcggcgatg aacttctccg tgttgaggtg cgtgccactg
    11821 ccgacgccgg cgtggcccca gttgatcgcg cccggccggg acttcgcgtc gttgacgatg
    11881 tccatgaggg tcttgtactt ggagtcgacc gtgaccacga gcacgttcgg ctgcagcgcg
    11941 aacggggcga tatcggtgaa gtccttggag acgtcgtagg gcagcttggc gtagatcgac
    12001 ggcgtcaccg tgtgcgcgtt cgaatcgacg agcagcgtgt agccgtcggg cggctgcttg
    12061 acgacgtagg ccgagccgat cgagccccct gctcccgccc ggttctcgac gacgaccggc
    12121 tggccccaga tctcggcgag cttctgcgtc accacgcgtc ccacgatgtc ggtggagctg
    12181 cccgcggtga acgacacgat gacgtgcacc ggcctgttgg ggaaaccctg cgcggacgcg
    12241 tgcccggcga gcgccgccgt caccgctgcg atcacgcatt tcagcatctt catctgctct
    12301 ctcctcggtg gacctatttg ttgtccgcgt tactgcggct tcgccccggc tgcgcgcatg
    12361 atcttgccga ggtcttcgta ttgctccttg atgaacgcgt cgaacttctc gggcgacatc
    12421 ggcatcggct cggcgcccag cttcgcgatc cgcgccctga cttcgggcgc ctgctccgcc
    12481 ttggcgatct ccgcattgag acgtgtcacg accgcgcggg gagtcttggc cggcgccagc
    12541 atcccgaccc agaaattgaa ctctccgccc gggtagcccg cctccttgat cgtcggcacc
    12601 tgcggcagcg tctccgagcg gttcggcgtg ctgaccgcga gcgcgaccac cttgccgtcg
    12661 cggatcgggc cgacgctcga gatcagcggc acccaggcca tctcgatgcg gcccgccatc
    12721 gcctcgttca ggatctcggg cgtgcccttg aacgggatgt gcactgcctg gatcccgctc
    12781 gcctggttga acttctcctg gttgatgtgc gccgcgctgc cgacgcccgc ggtgccgtag
    12841 ttgagctgac ccggcttcga cttcgccagc gcaaccaggt cgcgcaccgt cttcacgccg
    12901 agcgagggcg cgacgatcag cacgctgggc agcgtcgcga gcggcgacac gccggcgaaa
    12961 tccctgatcg tgtcgtaggg aagatccttg tacaggacgt ggttgaccgt gtgtcccgtc
    13021 gacacgacgc cgagcgtcag cccgtcgggc gcggccttcg cgaccaggtt gatgccgatc
    13081 gtgccgccag cgccgggatg gttctcgacg atcacgggct tgccgagcgc gctcgagagc
    13141 ttctcgccga ccgtgcgcgc catgatgtcg gttgcgctgc ccgcggtgaa cggcacgatg
    13201 accttcaccg gcccggacgg atagctttgc gcgtgcacgg cacctgcgac cgcgagcgca
    13261 gcgacgacgg cgagcgccgc cctggatgct ttcatctctc tctcctcttc gcttgtgtca
    13321 tgcggccgtt tcggtcttct tcttcttgct cttgccggcc cagtagtcgt cggccgattt
    13381 ggtcttcttg agcgcatcca tcgtctcgta ccccggggcc atcgacgccg acgtgtcggc
    13441 gagcacctgg tgatgctcga tgttgagcag ccgcagccag ctctgctgtc cgagcgtgag
    13501 cccgatcatg cagccgagct ccacgagctc gggctcgctg aagtgccgct tcatgcgctc
    13561 ccagaaggcg tcgtcggtct cggtgcgcca gacgatcgcc tcggcgtatg cgagcgccgc
    13621 cttctggcga tcgtcgtact tcgtggactt ctcgaagttg agcaggtcct tgacgtgatc
    13681 ctcgatcacg gcgccgctcg tcgtcgcgcg caccgagcgc tggttgccgc agtactcgca
    13741 ctcgaccgag cgcgacacgt acagccggca cagctccttg atcgcgtggt cgagcacgcc
    13801 gttcctgaag atgtcgcgcc acgagttggc gaagaaccag aagcaagccg gcacgtgcgc
    13861 gcgcacggcc gagctctcgg gccgcggcgt gccttcgcgc cggcagcggt ccatctcctt
    13921 ctgcatctgc gcgtccatct tgtcgagcgc aacgtagctg attctctgct tggccatggg
    13981 gtctcctgcg aaagttcagt ggatcgaaat ctgcctggtg gccgacagtt tagcaaggtc
    14041 tacgcaggcg atatgatcgt cgaccgagca gcccacccag gccatgccgg cgcagaacgc
    14101 cgtgtgcgac gaaccccgtc ccaccgcgac gcgtcccagc tcgcgcggcg tacgcgcgtc
    14161 gagcacctgg atctccgcgc cgccgaacgc ggtgacgacg acgtgccgtc cgtcgggcga
    14221 gacgtcgagc ccgtgcggat aggggccggt ctcgatcagg gccactcgct cgagcgtttc
    14281 ggcgtcgaag acgcacacgg tgtcgtcgag gaagttcgcg acgaagacgc gcgtcccgtc
    14341 agggctgacg tcgatcgcgt gggcccagcc gccgctcggc gcggcggcga cgatctcgcc
    14401 ctcggtaccg acacaggcga cggtgccgtc ccagctgttg gcgcagtaca cgcggccgtc
    14461 ggcccggcgc gcgaggtgcg acgggccctc gcccacccgc gtgcgcccga cgatgcggcc
    14521 gtcggcgagg cgcaccttca ccagctcgcc gtcgcccgcg caggggacga gaacgtgggc
    14581 cgcgtcgggg tcgagcagcg ggtcgtgcgg acacgtgccg acctccacgg tgcgctcgct
    14641 gtatccgccg cgccgcggcg tcgcgatggt cagcgtgccg gtgcctcctc ccgtgacgca
    14701 gacgacgccg tcgggcgcgg cgaggggcag ctgcgggcct cccggcgcgc gccagcgcgc
    14761 gatcacgcgc aggtccgcgg cgtgcagggc gatcacttcg ttgccttgcg cgtcggcgat
    14821 gaacagggtt ccggagcggt gctcgagcgc gagatgcgaa gccttgcgcg cgacgcgcgc
    14881 gaccgcgacc ggctcgccgc cgacgccgat gcgcgcgacg cgctcgccgc gctcgcaggt
    14941 ccacacggag tcgccgtcgg tcacgacgtg gtgcacgccc gggatcgttc cctgggcggg
    15001 cactgcgccg cgccggatcg cgagccggcg gccgcgctcg gcggtcagct ccgcggcgtg
    15061 gcggatgccc gcctcgagca gcgacacgcg cagcggcggc ccgtagtcgg cgaccagcgc
    15121 ctcgaaccgg gctcccggct cgagcgcgtt gaagcgctcg acgatcgcgc gtgcggcgtc
    15181 ggcaggcggc aggcggcggc aatcgagaac gggggcgctc atttcttcag ctcgactttc
    15241 tcgaagcccg ccggcagccg cggctgcccg gcttccccga gcgccggcgt ctcgctctcg
    15301 ccgtgcaccg tgtacaggcg cggcgccgcg cggttgatgt gcagctggaa cacgtcgggc
    15361 cggttgtagt gaccggcgaa atcgtgccgc agcttggcct ggacgccgat cgcaatgtcg
    15421 cactcggcgt acaggatacc ctcctccggg cccatcgggc ccgcgagcac gcggctgttc
    15481 ggcgcgacga tcgcactccc gccgcagcaa tcggggctgc gcaggaactt ctcggcctcg
    15541 gggccgacct tcagcatccc gatcatggcc tcgtccaccg tgccgcaagc cgagacgacg
    15601 aaggccttgc tcatctgcgc gaacgcctgc gagtcgacca gcacgcgatt gcgcatcggg
    15661 tcgccgctcg tcgggaaatg gttcggccag ctcatcacgt ggatgcgcgt gccctcggcg
    15721 gtgagcgcga acaccgcgag cggattcgag ttctccccgc agatcagccc gctcatcggg
    15781 ccgaactcgg tctggaaggc gccgaaggtg tcgccgaagc cgcccgcgtg caccaggcgc
    15841 tcgccgacgg tcggaacgag cttctggtgc ttgccgagga gcgtcccgtc ggggccgatg
    15901 tagacctgcg tgttgaacat cgtgccgatg gtgcgcggca gcttctcgca gacgccgacc
    15961 acgacgtagg ccttcgcatc gcgcgccgcg gcgcacaacg cgtcgatctc cggcccggga
    16021 atctcgaccg agttgcggaa cagcgccgcc gcgagctcgt tggcgaccgc gcccgtggct
    16081 gcatggtggt gataccagac cggatgcgcg gggatgaagc cctcgggaaa gccgatcacg
    16141 cgcgcgccgt tccggcccgc ttcccggatc agccggcacg ccttctcggt cgatccttcg
    16201 cggtccagga agaccgaagc cgcctggacg gcggcgacct tgacgacggg atacttgtca
    16261 cccatggcgg gaacggttgg tacggccgag agggcgaatt gtcgcacatc gcgccgccgc
    16321 ttccgaagcg cgtcggcatt ggaggccgtc cgtgttctaa gacgtccgct cgctcttgcg
    16381 gatgacgagc ttcaggccgg accagacctc gctgaccgcg cacaccttga cgtcgacgaa
    16441 gccgagcgga agcgcgaccg cgcggatcac gtcctcggtg atgtcggtcg gcactttcga
    16501 agccttcttc ggccaggaca cccagacgaa ccccgtctgc tcgaggcgct tgcgcagcga
    16561 ggcgagctgc ttttccaggg cgccgcgatt cgtcgcgaac aggtgtacgg cgcgcagcgg
    16621 tgcgctcgcc ttggaagcga gatcgattcc gggcgcaagc ttgccgagcc aggagggata
    16681 ttcaagcggc gcgttcagcg caaccagggt catcggcggc ttgaggccga gcttccctgc
    16741 gagcggcgtt cccgaatagc ccgccatgtt ctgtcactcc ccgacgaagc gctcgacctt
    16801 gccggtcttg atccacgcga cgatcgcccg caccacggcc tcctcgcgcc cctcgaacga
    16861 gtggaaatgg ccgccgccgc acggatctcc gtggggcgtc ccgccccgca ccgtcaccag
    16921 gttctcgccc gcgtacgcct tgaccgcgga atagggcgtg tatggacagg catcgttctc
    16981 gttgtgcacg tgcagcgccg gcgccttgat ctccccgtag gggaagccgg acaccgagcc
    17041 tgcgaagccg tcgcccgtgc gccggttcat cgacgccgag tggatgctgc cgctgatctc
    17101 ggtgccgaga ttcttcgcga gccagcgaga gctcaccgac cccatgctgt gccccatgat
    17161 gtagatccgg gtgaatccgt gctcgctctt cagcttcgcg atgatcttcc tcacgtcgtc
    17221 cgcgtgctgc tgcgaggagc gatagccgtc gaagcaggcg gtcggcttgg gcgagttccc
    17281 gccccattgg tcggtcggac agtcgacgac caccagcgca atgccctcct cggcgaggat
    17341 ctgctgattc ttgcgcatga acggctgcag gttgcgctgc ttgtcggcgg cggactggat
    17401 cctcgcgatg ccggggtagc cgcggaagta cagcagcgcg gtgtcggtgg gcctggcggg
    17461 catcgccagg atcgcccgct cgagcacggg cgatttcgtg aatacgcctt cggtgcgtcc
    17521 cgtgttcacc tcgatgagca catcggtcgt ctgcggccgg gcgacttgcg cccagagcag
    17581 ggcggcaagc aggagccacg cgttcttcct cattggatct cccgggctct tcgcgagcgg
    17641 aagcgagcta gcgtcgaggg tcggcgcgca ccgacgtcat tctcatcagc accgggacct
    17701 tcgcgttgac cgtcatgcgc ccggagatcg taccgatgac cgtcacggga cggttctcca
    17761 gggaggcgag ccgggacccc ggcggcggca gcagctgcac cgacaggacc tggcgggtga
    17821 tcgtgatgcc gccgcgctgg ccttcgatgc aagtcggctc gtacaaatcg agaatgggct
    17881 ggcgctccgc gcgatcgcca gcctcgatgt tctcgtaatt tggagcgccc ggggcgaccc
    17941 tgaaatggac gatccccttc agcgtggctt cgctttcgta cggcacgcac tcggcgccgg
    18001 ccgccgccga ggccagcgcg aacacgatcg cgaggaacaa tcgcatcgac gctccccgat
    18061 caggacgaca atccgtcgcc caccttcacg ttctcgggct gcagcgcgag gccggacgcc
    18121 tgcgcctgct gcacgagcag cttggagaag tcctcctcgg tcatcccgcg gcccatcatc
    18181 gactggatca ggtcgcgcgt gatcgacgcg agcggcatcg gcacctcgaa cttgcgcccg
    18241 gcctcgagcc ccaggtcgag gtccttgcgc agcaggtacg gcgtgaaggt gacgtggaag
    18301 tcgaggttga cgaacgcggg cgccttgtag cgcgtgaagg tcgatcccat cacgctcttg
    18361 ttcaggaact cgaggaaggc gtggcgcggg acgcccgcct tctccgcgag cacggtgatc
    18421 tcagccatgc actgcgtgac cacgccgagc atcacgttgt ggcagatctt cacgatgcgc
    18481 gacagctcgc cctcgccgac gtacgagccg ccgagcgggg cgattctcgc gagcagcggc
    18541 gcgacttcct cgtaggcctt cttcggaccc gagcacacga acgtcagctt cccggccttg
    18601 atgaccttgg cgttgcccga caccggcgac gccacgtatt cggtcccgta cggcttcagg
    18661 atctggcgca gctcggccga gccctcgagc gagatcgagg agcactccac gacgatgcgc
    18721 ggcgccgggc ctttccccgg ctgcgatttc gatagcagtc ccgccggtcc ggagacgact
    18781 tccttcacgt cgttccaggt ggacaccatc acgaacacga tgtcgcagcc ggcgagcgac
    18841 ggcaggtcgg cggcgatctt cgcgccgtcc ttcgcgagcg gctcggcttt cgcgcgcgtg
    18901 cggttccagg cgctgatgtc gcagccggcc tgcgccaggc gctgcgccat ctcgaatccc
    18961 atgcggccgg tgccgatcca gccgatcttc ggtttccttt cagccattac gtcttccttt
    19021 gcctcgatgc gatcaatatg gattggcgat cggcagctcg tccgccgggt agagcgagat
    19081 gatcttcgcg ccctgcggcg tgaggatgac ttcctcctcg atgcgcgccg cggagtagcc
    19141 gtcggtcgcg ggacagtagg tctcgaccgc gaacaccatg ccggccttga gctcgatcgg
    19201 ctccttgagc gacgtgaggc gcgagatcag cggccgctcg tgcagcccga gcccgaggcc
    19261 gtggcagaag ttcagcccga acgcctccat ctcggaggag aagccgatgt ccttcgcctc
    19321 gggcagcgcg cgcgcgatcc ggtccgaggt catcccgggc ttgagcagct cgatcgcctt
    19381 gtccatccac tcgcgcgcct tgcggtacgc ggtgcgctgc gcgggcgtcg ccttgccgac
    19441 gttgaacgtg cggtagtagc acgtgcggta gcccatgaag gactggatga tgtcgaagaa
    19501 cgcctggtcg cccgggcgga tgagccgatc ggtgaagttg tgcgggtgtg gcgagcagcg
    19561 ctcgcccgag atcgagttga tcgcctcgac gcagtcggag cccatctcgt agaggcgctt
    19621 ggtcgcgagc gcgacgatct gcgactcctg cacgccgggc ttcaacgcct cggcgatgtc
    19681 ctggtacacg ccgtcgacca tggccgaggc catgttgagc agcgtcagct cgtcgatgtt
    19741 cttcacctcg cgcgcggcga gcatcacctg ctggccatcg cgcacctcga tgccgagctt
    19801 ctgcagctcg aacagcatcg gcggctcgac cacgtccagg ccgagcggca tgtcgcccac
    19861 gccctcggcg tcgaggatgc ccttgatctc cttcgccgcc tcgcggaaga gcgtgatgtc
    19921 gcctcccgtc gcgccgcgca ggccgagcag cccggccttg cagtgatcga cgtgcagcca
    19981 gggcgcgtac aggcgatggt gcttggcggc gctgccgaag tcccagatgt agggatcgcc
    20041 gttcccggtg aggagcgagt agcgcgtgag cttgtc
//
LOCUS       DATDMW010000031         3225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1252484, whole genome shotgun sequence.
ACCESSION   DATDMW010000031 DATDMW010000000
VERSION     DATDMW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252484"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VI319_01065"
     CDS             complement(<1..1458)
                     /locus_tag="VI319_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY6862461.1"
                     /translation="MPRELAKILADCRDITVHRLLLSFTALCDRVCDMLMERAGRSVV
                     RDEQQLFLEARQVLIGGRAGLMSEFEKRLRSRIEDQVSGRADERADFSKADATKLALV
                     DLKIVDESVIVGNIVRVVENVCHDELVALNRGIGHLLGKSVVENAANPLGPETIVRAF
                     AEALDTLSAEPRVKFQILKELNQAPLGEFASIYGDVNRHLANLRIIPPTAGRKPAAPR
                     GSSPAPRKREGGDKPDAPQPSETDVMAMFRRAASHGGFPLAGHPAPPTRVYPSLPPTP
                     SGYIPGNPIVATPGLREGLTRLQAGQTDFNVEGMQVAFSGIPEGTHNVLRDLQESPLG
                     QRANQLEAMTIEMVAMLFDFIFDTRDLPDGIKALLARLQIPVLKVAMLDGAFFAKKTH
                     PSRLLVNALAAAGLGWSPAMGQEDPLYRKIENIVHRVIDGFGENLGLFDELRGELEAF
                     LASEEQAAEANFSTTAEAINEDDRKDLASVIAKSEV"
     gene            complement(1570..>3225)
                     /locus_tag="VI319_01070"
     CDS             complement(1570..>3225)
                     /locus_tag="VI319_01070"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEY6862462.1"
                     /translation="GFYHLALATRVPLGLAFIDYGRREIGIGAYLELTGDAAADMAAI
                     AAFYAERIPEDVHSRATFERWRRHDERAHPKRLFMTRELLMRHAASSVTEALFPDTLS
                     IAGAALPLKYRFAPGHPLDGLTLTVPLALLNQIDEATLSWLVPGMVRDKVTLYLRSLP
                     KAWRNRLVPLPDAVTDFLERSPRHDKALGDALRDYLQERLREAPPAEAFRDVELPPHL
                     AVNVRVVDASGQELAMDRDVAALRARLSEAARLEFAAEGPAFERRGLRQWEFGELPES
                     LSMRRDGQRRTGYPALVDETDGVALVLLDTREAALAATRKGVVRLIGFELGDSLGRMV
                     KAAGWAPIAMALRGVVPLDSLQEDFGHAVADRAYVGDDGLPRDRGAFVAQVKRARARL
                     PAVFDATLATTSAIASAHHALSQKIAALPAAQKMLAAEAKAQRDALVYPGFLAATPWP
                     QLAHLPRFLEALTRRIDRYAQNPERDRRHAAQVGAWWARYRERAEAERRAGRFSPALD
                     DFRWLIEELRVSLFAQELHTSVPVSQKRVEKAWQALPPSGARR"
BASE COUNT          499 a         1118 c         1129 g          479 t
ORIGIN      
        1 gacctcggac ttcgcgatga cgctcgcaag gtccttgcga tcatcctcgt tgatcgcctc
       61 ggccgtcgtc gagaaattcg cctcggccgc ctgctcctcg gacgcgagga acgcctcgag
      121 ctcgccgcgc agctcgtcga acaggccgag gttctccccg aagccgtcga tgacgcgatg
      181 cacgatgttc tcgatcttgc gatagagcgg atcctcctgg cccatcgcgg gcgaccaccc
      241 caggcccgcg gccgcgagcg cgttgaccaa gagccgcgac ggatgcgtct tcttcgcgaa
      301 gaacgcgccg tccagcatcg cgaccttgag caccggaatc tgcagccgcg cgagcagtgc
      361 cttgatgccg tcgggcaggt cgcgcgtgtc gaagatgaag tcgaacagca tcgcgaccat
      421 ctcgatcgtc atcgcctcga gctgattcgc gcgctggccg agcggcgatt cctgcaggtc
      481 gcgcagcacg ttgtgcgtgc cctcgggaat gcccgagaac gcgacctgca tgccctcgac
      541 gttgaagtcg gtctggcccg cctgcagccg cgtcagccct tcgcgcaggc ccggcgtggc
      601 gacgatcgga ttgccgggga tgtagcccga cggcgtcggc ggcagcgacg ggtacacgcg
      661 cgtcggcggc gcgggatggc ccgcgagcgg gaagccgcca tgcgacgcgg cacggcggaa
      721 catcgccatc acgtccgttt cggacggttg cggcgcgtcg ggcttgtcgc cgccttcacg
      781 cttgcgcggc gcgggactgc tgccgcgcgg cgctgccggc ttgcgcccgg cggtgggcgg
      841 gatgatgcgc aagttggcga gatggcggtt cacgtcgccg tagatcgacg cgaattcgcc
      901 gagcggcgcc tggttcagct ccttcaggat ctggaacttg acgcgcggct cggcggaaag
      961 cgtgtcgagc gcctcggcga aggcgcggac gatcgtctcg ggaccgagcg ggttcgccgc
     1021 gttctcgacg acggacttgc cgaggagatg gccgatgcca cggttcagcg cgacgagctc
     1081 gtcgtggcat acgttctcga ccacgcgcac gatgttgccg acgatcaccg actcgtcgac
     1141 gatcttgaga tcgacgagcg ccagcttcgt cgcgtccgcc ttggagaagt cggcgcgctc
     1201 gtccgcgcgg cccgacacct ggtcttcgat ccgggagcgc aaccgcttct cgaactcgga
     1261 catcaggccc gcgcggccgc cgatcaggac ctgccgcgcc tcgaggaaca gctgctgctc
     1321 gtcgcggacg accgaacggc cggcacgctc catcagcatg tcgcagacgc ggtcgcaaag
     1381 cgccgtgaac gacagcaaca gccggtggac cgtgatgtcg cggcaatccg cgaggatttt
     1441 cgccagctcc cggggcacgg gacggcgagc cggcgcaacg tgggtcgtta cgtttgggga
     1501 aggcacaggc ttgctgccgg cttcattggc gtttcgactc tagcacgggg acgcattgta
     1561 tgccaccaat caacgcctcg cgcccgaggg aggcagcgcc tgccatgcct tttccacccg
     1621 cttctgcgat accggtacgc tcgtgtgcaa ttcctgcgcg aagagcgaca cgcgcagctc
     1681 ctcgatgagc cagcggaaat cgtcgagcgc gggcgagaag cgccccgcgc ggcgctcggc
     1741 ttccgcgcgc tcgcggtagc gcgcccacca cgcgcccacc tgcgccgcgt ggcgccggtc
     1801 acgttcggga ttctgcgcgt aacggtcgat gcggcgcgtc agcgcttcga ggaagcgcgg
     1861 caggtgggcg agttgcggcc agggggtggc ggcgaggaag cccgggtaga cgagcgcatc
     1921 gcgctgcgcc ttcgcctcgg cggcgagcat tttctgcgcc gcgggcaacg cggcgatctt
     1981 ctgcgacaac gcgtggtgcg ccgatgcgat cgccgacgtg gtcgcaagcg tcgcgtcgaa
     2041 gacggccgga agccgcgcgc gggcgcgctt cacctgcgcg acgaacgcgc cgcgatcgcg
     2101 cggcagcccg tcgtcgccca cgtacgcccg gtccgcgacg gcgtgcccaa aatcctcttg
     2161 caggctgtcg agcggcacga cgccccggag cgccatcgcg atcggtgccc atccggcagc
     2221 cttcaccatt cgcccaagcg aatccccgag ctcgaagccg atcaggcgca cgacaccctt
     2281 gcgcgtcgcc gccagcgcgg cctcgcgcgt gtcgagcagc acgagtgcca cgccgtcggt
     2341 ctcgtcgacg agcgccggat agccggttct gcgctggccg tcgcggcgca tcgacaacga
     2401 ctcgggcagc tcgccgaatt cccattgccg caggccgcgg cgctcgaacg ccggcccttc
     2461 ggcggcgaac tcgagccgcg ccgcctcgga aagccgcgcg cgcagcgccg cgacgtcgcg
     2521 atccatcgcg agctcctgcc ccgacgcgtc gacgacgcga acgttgaccg caagatgcgg
     2581 cggcagctcg acgtcgcgaa acgcctccgc cggcggcgct tcccggaggc gttcctgcag
     2641 gtagtcgcgc aaggcatcgc caagcgcctt gtcgtggcgc ggcgaacgct cgagaaagtc
     2701 ggtgaccgcg tcgggcagcg gaaccagccg gttgcgccac gccttcggca gcgagcgaag
     2761 gtaaagcgtg accttgtcgc gaaccatgcc gggcacgagc cacgacagcg tcgcttcgtc
     2821 gatctggttc agcagcgcga gcgggacggt cagcgtcagg ccatcgagcg gatggcccgg
     2881 cgcgaagcgg tacttgagcg gcaacgcggc gcccgcgatc gacagcgtgt cggggaacag
     2941 cgcctcggtc accgagctgg ccgcatgccg catcagcagc tcgcgggtca tgaacaggcg
     3001 cttcgggtgc gcgcgctcgt cgtgcctgcg ccagcgctcg aacgtggcgc gcgaatggac
     3061 gtcctcggga atgcgctcgg catagaaggc ggcgatcgcg gccatgtcgg ctgcggcgtc
     3121 cccggtcagc tcgagatacg cgccgatgcc gatctcgcgc ctgccgtaat cgatgaaggc
     3181 gaggccgagc ggcacgcgcg tggcgagcgc gaggtgatag aagcc
//
LOCUS       DATDMW010000032        12411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1750718, whole genome shotgun sequence.
ACCESSION   DATDMW010000032 DATDMW010000000
VERSION     DATDMW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750718"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VI319_01075"
     CDS             <1..481
                     /locus_tag="VI319_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY6862463.1"
                     /translation="IGEITIPENEPGSSIMPGKVNPTQSEAMTMLCAQVMGNDVAINI
                     GGASGNFELNVFKPLIAQNFLQSARLLADGCDSFRKNCAAGIEPDRERIAELMRRSLM
                     LVTALNPRIGYDKAAKIAKKAHAEGTTLREAALALGYVTAEQFDAWVRPEDMVGRRD"
     gene            621..992
                     /locus_tag="VI319_01080"
     CDS             621..992
                     /locus_tag="VI319_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6862464.1"
                     /translation="MWAEALEMLQSAERLQRQFFQIGAADAPWEPPVDVYQAGDDLWI
                     FLALPGVPADHMETIIDGATLVVRGARPLPPAARGARIHRLEIPYGRFERRITLPAGH
                     YQVLQQVVESGCLVLGMRKLT"
     gene            989..3427
                     /gene="lon"
                     /locus_tag="VI319_01085"
     CDS             989..3427
                     /gene="lon"
                     /locus_tag="VI319_01085"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY6862465.1"
                     /translation="MNEINSALESNRSATDAGVNAAPAARSGGDVSLPDDALILIPVR
                     NLVMFPGMILPVSLGREGSIAAAQAAMKSERPVGLLLQRNPEAELPTADDLTRIGTVA
                     GILRYVTTPDGSHHIVCQGQARFQILEYLFGYPFLAARVQRIAEPAETSKEIEARFLQ
                     LKARAGEALELLPQTPPELAATVQTMKAPGTLADLVAGFMDATPAEKQSVLETIGLEP
                     RLDAVLKLLANRIEVLKLSKEIDERTKASIDERQREYLLREQMKAIRKELGEGEEGNA
                     AAEIEELRKAIAEAGMPEEVEKQATKELKRLERMSESSAEHSMVRTYLDWLIELPWKD
                     PEADRIDMAEARRILDEDHYGLETIKKRILEFLAVRKLNPTGHGPILCFVGPPGVGKT
                     SLGQSVARALGRKFTRVSLGGVHDEAEIRGHRRTYIGALPGNIVQALKKCGTRGCVLM
                     LDEIDKLGAGIHGDPSAALLEVLDPAQNNTFRDNYLAVPYDLSKVLFITTANVLDAIP
                     GPLRDRMEIIQLTGYTQEEKREIARRYLLKRQLEENGLKADQLEITDAALAAVIRDYT
                     REAGVRSLERQIGAVARRAAVTIAEGKATRVRIDEADLAAILGAARFENEVALRAGSP
                     GVATGLAWTPVGGDILFIEATRTPGGGRLILTGQLGEVMKESAQAALTLVKSRAARLG
                     IDPAGFEKTDVHIHVPAGAIPKDGPSAGVAMFIALASLFADRPVRSDTAMTGEISLRG
                     LVLPVGGIKDKVLAAMRAGIRTVMLPSRNRKDLEEVPAEAKEKLEFVWLEHVDDAMRA
                     AVAGGAALPEVAHV"
     gene            3628..5034
                     /locus_tag="VI319_01090"
     CDS             3628..5034
                     /locus_tag="VI319_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862466.1"
                     /translation="MEAEQLLTEISDYCRRVGMAESTFGRRAVNDGKLVSRLRIGGRI
                     TTDTAERVRSFIRQNPNLEGRNFARLRAAAGELRPAAAPAVAAQAPMAAPVNGAHNFR
                     FYDNRQKYLLFVNTCSEKWVVAQRVGMELASIHPRPPAVRLFDAGVGDGTVLARVMRS
                     MHTRFPTMPFYIVGKEISLEDVRLALEKMPDRFYEHPATVLVITNLRYSEAPWLTPDS
                     VTAATSMVWRELALTGNTASEFEQQITDLQGFLADNWRATIGKAGNPVYEKPVVLVIY
                     REDYRFLLDTVRPQRGHVRADYDLVIASQPYRLRAPLEFKARAVLAPLARSLAPAGRL
                     LAIHSHGADPGLEIVQKVWPGENPFQHDRHALLKATRAELGNDGRDLNFNAYADNRSL
                     FRYDMHTLPSEIGDTIGTSTLLAAWNAAVYVNQIEDGRLAEVMGDRRYLEATSEVLHR
                     HGGLWFLDESFVVSRKRD"
     gene            5064..6005
                     /locus_tag="VI319_01095"
     CDS             5064..6005
                     /locus_tag="VI319_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862467.1"
                     /translation="MSTPVIDRSATPSPAQAEHGAIAALVEGASLEISAHDAADIAEC
                     AGYLDPGTEVFISQPMGQPYHGAVALAARLRRAGFRPVPHVTARALASLSALEDYLAR
                     ATGEAGVEHVLVIGGDRDRPAGPFDSSLAVLCTGALQRHGIRKLSIGGYPEGHPAIGP
                     KALRDALRAKRELARENGLQLQVVTQFAFDAQPVVAWTRSIAALDVPVRVGVAGPASI
                     TTLLKFAARCGIGNSVRVLKSRSDSIIRLITEAGPETVIRDLALGAAAGEARFAGVHL
                     YTFGGLLRSARWLRAVRSGNFQIVPGQPGFRVGTSAG"
     gene            6413..8359
                     /locus_tag="VI319_01100"
     CDS             6413..8359
                     /locus_tag="VI319_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEY6862468.1"
                     /translation="MNELASNGHAERREKFLQSLVSFSKDHRAARWSGSFAQFLESIL
                     PAAPLAVARSSHEYIWDMIRSEGYDDSDGRFRCKLFEDELFGIDETLERLIDYFKAAS
                     AGSEVGRRLLLLLGPPSGGKSSLVILLKRGLEEYSLTDAGAIYGIQGCPVHESPLHLV
                     PHTLRPSFRESYGVEISGELCPYCATRLAQQYGGDFMQMPVQRVFISEAGRVGVGTYA
                     PHDPTTADIADLVGSVDLSKVAEYGDEGDPRAWSWSGAVYAASRGLLEMIEILKVKRE
                     FLYLLLTLTQEKNVKVSRFPLIYLDETIVAHTNLAEFRKFLQEAENEALLDRMVIIQV
                     PYTLSYRDEARIYRKLTSGAQAFKEVHLDPHALHVAAVFATLTRLQEGEEKEAELVRK
                     VRVYAGEDVEGVSRTDADKMRARAGEEGLSGVSPRFVINALSNAIIQSDRKSLTSMEV
                     LLALKDSIESDARIDGKKKRKWVDFLVIARKEFYNRWVKEDVHKALFVSFEQEAQELL
                     DKYLDEVEAMLDSRQIKDPITSEERRPDERFLRSVEEKIHISDSGKLSFRQEVVRKAM
                     GSYKRGEKFSLDSHAQLHDAVKQYLFEQRRDVLRLVSSAARPDEEVKQKISVVEKRLV
                     DEYGYDPHSAREALNYVTTLLAQE"
     gene            8375..9565
                     /locus_tag="VI319_01105"
     CDS             8375..9565
                     /locus_tag="VI319_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEY6862469.1"
                     /translation="MDPLDPASPRTRIAAETPWYELFSRGARDWLRHNDKVREAVTGN
                     LPELLSGSDILTQPTGRTVLVPVKLLEHSRFRLREAQTQTGAGQGQGEPGDVLRPAQQ
                     HGEDAGEGKGGTGEGELTFVVELKIDDILDWLWEELKLPDLKPRTSANMQDDELVREG
                     WDKRGPRARLDRRRTVKEAVKRRAIQYREDRKEIPFTNDDLRFRQLVRRQRPAVNAVV
                     MFALDVSGSMGEAERKLAKMFFFFALHGLRRQYANVEPVFLAHSVEAWEFPEDQFFQV
                     SGSGGTVSSSVFNLAIETLNTRFDPSRYNAYLFYASDGENASEDQEPAAASMERLAGR
                     LNYAGYAEIGVTGSRLRQTPLGALFTDLQGRGFPAAMATIARQDDVWRAIRTFFSEQA
                     EATA"
     gene            9562..10920
                     /locus_tag="VI319_01110"
     CDS             9562..10920
                     /locus_tag="VI319_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY6862470.1"
                     /translation="MTQLQEYASKIEELARELGLDYYPVDFNVTPSSFMIEIAAYGLP
                     VRMPHWSFGVRYIHQLVRQSMGHSKIFEVMFPGNPCYAYLMDTNTVAENSLVTAHVVG
                     HADFAKNNQLFARYHRQAGGNIVEQAAAHAHRIESAVSEFGLQAVESTLDAALALEAH
                     VDIDQEMHRERYPERGPEGGAAPADMFHERFNRLPGEAAPQQAPRQAPRLPVPPRPEY
                     DLLWFIAQYAPELEQWERDVFLAVREESMYFYPVFACQIMNEGWASYWHARLLREADF
                     LPHNLYLSALKSHSDVVRPFASNQNTALAVNPYHLGFSLWEHLVQTAGMEKAREICRD
                     EDDFGFIRNYLDRDLADKLKLFVYEADADGEIRVAGRDINQLREAILAPKFNYGAPRI
                     AVSELRHDGTLVLVHDNAGDGRGLDLDRAARVLQYIQRVWRRPVRLHTVDEFGRNREL
                     AA"
     gene            10963..11949
                     /locus_tag="VI319_01115"
     CDS             10963..11949
                     /locus_tag="VI319_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862471.1"
                     /translation="MRSVVRLLAAVAGTALIAALFANEALAQNARNYPDRPVKLVVGY
                     APGGLPDTVARIVGQALGERWHQQPVVENRPGANGIVGAEVVVKSAADGYTLLVTDNS
                     TTAINPFLYKKLPYSSKDLAPVSMIARAPLYLAANPSFPANSLKEMIDLVKANPGKYS
                     YGSSGIGSTHHLCQEFMNAALGLQITHVPYKGTGQSVPAVVAGQVAMAWSAGPSLAAY
                     VKDGRLKLLAVNSIKRTPAAPNLPAVSEVIPGFNFSPTIGVFAPAGTHKDVIGRIASD
                     VAEAVKVPEVVTRLTNLDIQPIGSTPEEYAADLRADDERYSKAVKISGAKVD"
     gene            11958..>12411
                     /locus_tag="VI319_01120"
     CDS             11958..>12411
                     /locus_tag="VI319_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6862472.1"
                     /translation="MAAVVKPDRAAFSRELEALDVRPLWERVMRLKPGTAAVPAMWRW
                     AQLRPRLARASELITAKEAERRVLMLENPALRGTTFATTTLYAGLQIIMPGEIAPTHR
                     HTPNALRFIVEGEGAYTAVDGERVAMRPGDFVVTPNWTWHDHGNAGSEP"
BASE COUNT         2029 a         4277 c         4309 g         1796 t
ORIGIN      
        1 catcggcgag atcacgatcc ccgagaacga gccgggcagc tcgatcatgc ccggaaaagt
       61 gaatccgacg cagtccgagg cgatgacgat gctgtgcgcc caggtgatgg gcaacgacgt
      121 cgcgatcaac atcggcggcg cgtcgggcaa cttcgagctg aacgttttca agccgctcat
      181 cgcccagaac ttcctgcaga gcgcgcgcct gctcgccgac ggctgcgaca gcttccggaa
      241 gaactgcgcc gccggcatcg agcccgaccg cgagcgcatc gccgagctga tgcgccgctc
      301 gctcatgctc gtcacggcgc tcaatccccg catcggctac gacaaggcgg cgaagatcgc
      361 caagaaggcg cacgccgagg gcacgacgct gcgcgaggcg gcgctcgcgc tgggctacgt
      421 caccgccgag cagttcgacg cctgggtgcg cccggaggac atggtcggcc gccgcgactg
      481 agcggcgctt gtggggtctt ttttcgtccc catttaccgg tttgatccgg atcaatccgt
      541 ggatgggccg cggtgcttga atctccggag aaaccggggg tcataccgag gcgatgcgtt
      601 cccgagatcc cagatcctgg atgtgggccg aggctctgga gatgctgcag tccgccgagc
      661 gtctccagcg ccagttcttc cagatcggcg cggccgatgc gccgtgggag ccgccggtcg
      721 acgtctacca ggccggcgac gacctgtgga tcttcctcgc gctgccgggc gtgcctgccg
      781 accacatgga gaccatcatc gacggcgcga cgctcgtcgt gcgcggcgcg cggccgttgc
      841 cgccggccgc gcgcggggcg cgcatccatc gcctcgagat cccgtacggc cgcttcgagc
      901 gccgcatcac gctgcccgcg ggtcattacc aggtgctgca gcaggtggtg gagagcggct
      961 gcctcgtgct cggcatgaga aagctgacat gaacgagatc aactccgcac tcgaatcgaa
     1021 ccggtcggcg accgacgcgg gcgtgaacgc cgcgcccgcc gcgcgcagcg gcggcgacgt
     1081 ctcgctgccc gacgacgcgc tcatcctgat cccggtgcgc aacctcgtca tgttcccggg
     1141 aatgatcctg ccggtctcgc tcggccggga gggatcgatc gcagccgcgc aggcggcgat
     1201 gaagtccgag cgcccggtcg gcctcctcct gcagcgcaac cccgaggccg agctcccgac
     1261 cgcggacgat ctcacgcgca tcggcacggt cgccgggatc ctgcgctacg tcaccacgcc
     1321 cgacggctcg catcacatcg tgtgccaggg ccaggcgcgc ttccagatcc tcgagtatct
     1381 cttcggttac ccgttcctcg ccgcgcgcgt ccagcgcatc gccgagccgg cggaaacgag
     1441 caaggagatc gaggcgcgct tcctgcagct gaaggcgcgc gccggggagg cgctcgagct
     1501 gctgccgcag acgccgcccg agcttgccgc cacggttcag acgatgaagg cgccgggcac
     1561 gctcgccgac ctcgtggccg gcttcatgga cgcgacgccc gccgagaagc agtcggtgct
     1621 cgagacgatc ggcctcgagc cgcggctcga cgcggtgctg aagctgctcg cgaacaggat
     1681 cgaagtgctg aagctctcga aggagatcga cgagcggacc aaggcgagca tcgacgagcg
     1741 gcagcgcgag tacctgctgc gcgagcagat gaaggccatc aggaaggagc tgggcgaggg
     1801 cgaggagggc aacgccgccg ccgagatcga ggaattgcgc aaggcgatcg ccgaggccgg
     1861 catgcccgag gaggtcgaga agcaggccac caaggagctc aagcgcctcg agcgcatgtc
     1921 cgaatcctcc gccgagcact cgatggtgcg cacctacctc gactggctga tcgagctgcc
     1981 gtggaaggat cccgaggccg accggatcga catggccgag gcgcgtcgca tcctcgacga
     2041 ggaccactac gggctcgaga cgatcaagaa gcgcatcctc gagttcctcg cggtgcgcaa
     2101 actgaacccg accgggcacg ggccgatcct gtgcttcgtc ggtcccccgg gggtcgggaa
     2161 gacctcgctc ggacagagcg tggcgcgcgc gctcgggcgc aagttcacgc gcgtgtcgct
     2221 cggcggcgtg cacgacgagg ccgagatccg tggccaccgg cgcacgtaca tcggcgcgct
     2281 cccgggcaac atcgtccagg cgctgaagaa gtgcggcacg cgcggctgcg tgctcatgct
     2341 cgacgagatc gacaagctcg gcgccggcat ccacggcgat ccgtcggcgg cgctgctcga
     2401 ggtgctcgac ccggcgcaga acaacacgtt ccgggacaac tacctcgcgg tgccctacga
     2461 cctgtcgaag gtgctgttca tcacgacggc gaacgtgctc gacgcgatcc cggggccgct
     2521 gcgcgaccgc atggagatca tccagctgac tgggtatacg caggaggaaa agcgcgagat
     2581 cgcgcgccgc tatctcctga agcgccagct cgaggagaac ggcctgaaag ccgaccagct
     2641 cgagatcacc gacgcggcgc tcgccgcggt cattcgcgac tacacgcgcg aggccggggt
     2701 gcgctcgctc gagcgccaga tcggcgcggt ggcgcggcgc gcggcggtga ccatcgcgga
     2761 gggcaaggcg acgcgcgtgc gcatcgacga ggccgacctc gcggcgattc tcggcgcggc
     2821 acggttcgag aacgaggtgg cgctgcgtgc cggctcgccg ggcgtggcga ccgggctcgc
     2881 ctggacgccg gtcggcggcg acatcctgtt catcgaggcg acgcgaacgc cgggcggcgg
     2941 caggctcatc ctcacgggcc agctgggcga ggtcatgaag gaaagcgcgc aggccgcgct
     3001 cacgctggtc aagtcgcgcg cggcgcggct cggcatcgac cccgcgggat tcgagaagac
     3061 cgacgtgcac atccacgtcc cggcgggcgc gatcccgaag gacggcccga gcgcgggcgt
     3121 cgcgatgttc atcgccctcg cgtcgctgtt cgcggaccgg ccggtgcgca gcgacaccgc
     3181 catgacgggc gagatcagcc tgcgcggcct ggtgctgccg gtcggcggca tcaaggacaa
     3241 ggtgctggcc gcgatgcgcg ccgggatccg cacggtcatg ctcccgtctc gcaaccgcaa
     3301 ggacctggaa gaggtgccgg ccgaggcgaa ggagaagctc gagttcgtct ggctggagca
     3361 tgtcgacgac gcgatgcggg ccgctgtcgc gggcggcgcg gcgctccccg aagttgccca
     3421 cgtctgactc gtccgagcgt gggaagcgcc tgtttctcag ggacttggcg cttgcctatg
     3481 cccactcgcc tgggtcgctt aacctagtcc attcaaactg atatacaggc ctgcccacga
     3541 atcgtcccac tctcgcgatg ggaccgaatg tggttgacct ttctcggtac aggggcatct
     3601 aattggggct ggaggacgga ccgccccgtg gaagccgagc agcttctgac cgagatctcg
     3661 gactattgcc gccgcgtcgg catggcggag tccaccttcg gccggcgcgc ggtcaacgac
     3721 ggcaagctcg tcagccgctt gcgcatcggc ggccgcatca ccaccgacac cgccgagcgc
     3781 gtgcgctcct tcatccgcca gaacccgaac ctcgagggcc gcaacttcgc ccggctgcgc
     3841 gccgccgcgg gcgagctgcg cccggcggca gcgcccgcgg tcgcggcgca ggcccccatg
     3901 gccgcgcccg tgaacggcgc gcacaacttc cgtttctacg acaaccgcca gaagtacctg
     3961 ctcttcgtca acacctgcag cgagaaatgg gtggtcgcgc agcgcgtcgg aatggagctc
     4021 gcgagcatcc acccgcgccc gcccgccgtg cggctgttcg acgcgggcgt cggcgacggc
     4081 acggtgctcg cgcgcgtcat gcgctcgatg cacacgcgtt tcccgacgat gccgttctac
     4141 atcgtgggga aggagatttc gctcgaggac gtgcgcctgg ccctggagaa gatgcccgat
     4201 cggttctacg agcaccccgc cacggtcctg gtcatcacca accttcggta ctccgaggcg
     4261 ccgtggctga cgcccgattc ggtgaccgcg gcgaccagca tggtgtggcg cgagctggcg
     4321 ctgaccggca acaccgcgag cgagttcgag cagcagatca ccgacctgca gggcttcctc
     4381 gccgacaact ggcgggcgac gatcgggaag gcgggcaacc ccgtgtacga gaagccggtc
     4441 gtcctcgtga tctaccgcga ggactaccgc ttcctcctcg acaccgtccg gccgcagcgc
     4501 ggacacgtgc gcgccgacta cgacctggtc atcgcctcgc agccctaccg cctgcgcgcg
     4561 ccgctcgagt tcaaggcgcg cgccgtgctc gcgccgctcg cgcgcagcct ggcgccggcg
     4621 gggcggctgc tcgcgatcca ttcgcatgga gccgacccgg gcctcgagat cgtgcagaag
     4681 gtctggcccg gcgagaaccc gttccagcac gaccgccacg cgctgctcaa ggcgacgcgc
     4741 gccgagctcg gcaacgacgg ccgcgacctc aacttcaacg cctacgcgga caaccgctcg
     4801 ctgttccgct acgacatgca cacgctgccg agcgagatcg gcgacacgat cggcacgtcg
     4861 acgctgctcg ccgcgtggaa cgccgcggtg tacgtgaacc agatcgagga cgggcgcctc
     4921 gccgaggtca tgggcgaccg ccgctacctc gaggcgacga gcgaggtgct gcaccgccac
     4981 ggcggactct ggttcctcga cgagtcgttc gtcgtctcgc gaaagcgcga ctagcccggc
     5041 ccctgatgcg gaagaaactg tccatgtcca cacccgtgat cgaccgttcc gccaccccct
     5101 ctccggcgca ggccgagcat ggcgccatcg ccgcgctggt cgagggcgct tcgctcgaga
     5161 tctcggcgca cgacgcggcg gatatcgccg agtgcgcggg ctacctcgat cccggaacgg
     5221 aagtcttcat cagccagccg atggggcagc cctaccacgg cgcggtcgcg ctggcggcgc
     5281 ggctgcgccg cgccggcttc cgcccggtgc cgcacgtgac cgcgcgcgcg ctcgcgagcc
     5341 tctcggcgct cgaggactat ctggcgcgcg ccacgggcga ggcgggcgtc gagcacgtgc
     5401 tggtcatcgg tggcgaccgc gaccggcccg cgggaccctt cgactcgagc ttggcggtgc
     5461 tgtgcaccgg cgccctgcag cgccacggga tccggaagct gagcatcggc ggatacccgg
     5521 agggccaccc cgcgataggc ccgaaggcgc tgcgcgacgc gctgcgggcg aagcgcgagc
     5581 tcgcgcgcga gaacgggctc cagctccagg tggtgacgca gttcgccttc gacgcgcagc
     5641 cggtcgtcgc ctggacccgg agcatcgcgg cgctggatgt cccggtgcgt gtcggcgtcg
     5701 cggggccggc gagcatcacc acgctgctca agttcgcggc gcgctgcggc atcggcaact
     5761 cggtgcgggt gttgaagtcg cgcagcgact cgatcatccg tctcatcacc gaggcgggtc
     5821 cggagaccgt gatccgcgac ctggcgctcg gcgccgcggc cggcgaagcg cggttcgccg
     5881 gcgtgcacct gtacaccttc ggcggcctgc tgcgcagcgc gcgctggctg cgcgccgttc
     5941 gcagcgggaa tttccagatc gtgccgggac aacccgggtt ccgcgtcggg acctccgcgg
     6001 gctgagccgc tcgcgccgcg gcgcgcggcg cgcttcctcc tgtcgtgctt cgacgacgcg
     6061 cgcgtcgcgc gatcttgcat cgcgcgcgcg tggtcaacgc gccgcagtca actcgggtgc
     6121 gcgccgggcg tttcaccaaa atgtaacgct ccgcgcgcaa ggtgatgcac ggtgtaagct
     6181 gaaaagtgcg cgcggaacgt ttgcatcgcc gcccatgtcc atagtcgtgc cgggacgtgt
     6241 ttggcgcgcg cggcgagcga gcggcgcatg gaactggata ggcacttgaa aacattgggc
     6301 tccccatcca cgctgggtgc ggcacagagc gcggggtgac gagatccgtg gcaggcccgg
     6361 agcttcggcg atcggtggat gaggaggcgc ggcaccgaaa cggagcaacg tcatgaacga
     6421 gctggcaagc aacggacatg ccgagcgcag ggagaagttt ctccagtcgc tggtgagctt
     6481 cagcaaggac catcgcgccg cacgctggtc cgggtccttc gcgcaattcc tcgaaagcat
     6541 cctgcccgcg gcgccgctcg cggtcgcgcg cagcagccac gaatacatct gggacatgat
     6601 ccgctcggag ggctacgacg actccgacgg caggttccgt tgcaagctgt tcgaggacga
     6661 gctgttcggg atcgacgaga cgctcgagcg cctgatcgac tacttcaagg cggcgagcgc
     6721 ggggtcggag gtcggccggc gcctgctgtt gctgctcggt ccgccgtcgg gcggcaagtc
     6781 gtcgctcgtg atcctgctca agcgcgggct ggaggaatac agcctgaccg acgccggcgc
     6841 gatctacggg atccagggct gcccggtgca cgagtcgccg ctgcaccttg tccctcacac
     6901 cttgcgtccc tctttccggg agtcctacgg cgtcgagatc agcggcgagc tgtgcccgta
     6961 ttgcgcgacg cgactcgcgc agcagtacgg cggcgacttc atgcagatgc cggtgcagcg
     7021 cgtgttcatc tcggaggccg gccgcgtggg cgtggggacg tacgcgccgc acgacccgac
     7081 cacggccgac atcgccgacc tggtcgggtc ggtcgacctc tccaaggtgg cggaatacgg
     7141 cgacgaaggc gacccgcggg cctggtcctg gtcgggagcc gtgtatgccg cgagccgcgg
     7201 cctgctcgag atgatcgaga tcctgaaggt gaagcgcgag ttcctgtacc tcctgctcac
     7261 gctcacgcag gagaagaacg tcaaggtgtc gcgctttccg ctcatctacc tcgacgagac
     7321 catcgtcgcg cacaccaacc tcgcggagtt ccgcaagttc ctgcaggagg cggagaacga
     7381 ggcgctgctc gaccgcatgg tcatcatcca ggtgccgtac acgctgtcgt accgcgacga
     7441 ggcgcgcatc taccgcaagc tcacctcggg cgcgcaggcg ttcaaggaag tgcacctcga
     7501 cccgcacgct ctgcacgtcg ccgcggtgtt cgcgacgctg acgcggctgc aggagggcga
     7561 ggagaaggag gccgagctcg tgcgcaaggt gcgcgtctac gccggcgagg acgtcgaggg
     7621 cgtctcgcgc accgacgccg acaagatgcg cgccagggcg ggcgaggagg gtctctcggg
     7681 cgtgtcgccg cgcttcgtca tcaacgcgct ctcgaacgcc atcatccagt ccgaccgcaa
     7741 gagcctcacc agcatggagg tgctgctcgc gctcaaggac tcgatcgagt cggacgcccg
     7801 catcgacggc aagaagaagc gcaagtgggt cgacttcctc gtgatcgcgc gcaaggagtt
     7861 ctacaaccgc tgggtcaagg aggacgtgca caaggccctg ttcgtctcgt tcgagcagga
     7921 agcgcaggag ctgctcgaca agtacctgga cgaggtcgag gcgatgctcg acagccgcca
     7981 gatcaaggac ccgatcacca gcgaggagcg ccgccccgac gagcgcttcc tgcgctcggt
     8041 cgaggagaag atccacatct cggattccgg caagctctcg ttccggcagg aggtagtgcg
     8101 caaggcgatg ggctcgtaca agcgcggcga gaagttctcg ctcgacagcc acgcgcagct
     8161 gcacgacgcg gtcaagcagt acctgttcga gcagcggcgc gacgtgctgc gcctggtgag
     8221 ctcggcggcg cgtcccgacg aggaggtcaa gcagaagatc tcggtcgtcg agaagcgcct
     8281 cgtcgacgag tacggctacg acccgcacag cgcgcgcgag gcgctcaact acgtgaccac
     8341 gctgctcgcg caggaatgag gcgccgcccc gaacatggat ccgctcgatc ccgcatcgcc
     8401 gcgcacgcgc atcgccgcgg agacgccgtg gtacgagctg ttctcgcgcg gcgcccgcga
     8461 ctggctgcgg cataacgaca aggtccgcga ggccgtcacc ggcaatctgc ccgagctgct
     8521 ctcgggatcc gacatcctga cccagcccac cgggcgcacc gtgctcgtgc cggtgaagct
     8581 gctcgagcac tcgcgcttcc gcctgcgcga ggcgcagacg cagaccggcg ccggccaggg
     8641 ccaaggcgag cccggcgacg tgctgcggcc ggcgcagcag cacggcgagg acgcgggcga
     8701 gggcaagggc ggcaccggcg agggcgagct gaccttcgtc gtcgagctga agatcgacga
     8761 catcctcgac tggctctggg aggagctcaa gctccccgat ctgaagccgc gcacgagcgc
     8821 gaacatgcaa gacgacgagc tggtgcgcga gggctgggac aagcgcggcc cgcgcgcgcg
     8881 cctcgaccgg cgccgcaccg tgaaggaagc ggtgaagcgc cgcgcgatcc agtaccgcga
     8941 ggaccgaaag gagatcccgt tcaccaacga cgacctgcgt ttccgccagc tcgtgcgccg
     9001 gcagcgtccc gcggtgaacg cggtggtcat gttcgcgctc gacgtctcgg gcagcatggg
     9061 cgaggccgag cgcaagctcg ccaagatgtt cttcttcttc gcgctgcacg gcctgcggcg
     9121 gcagtacgcg aacgtcgagc cggtgttcct cgcgcactcg gtggaggcct gggaatttcc
     9181 cgaagaccag ttcttccagg tgagcggctc gggcggcacg gtttcctcga gcgtgttcaa
     9241 tctcgcgatc gagacgctca acacgcgctt cgatccctcg cgctacaacg cctatctctt
     9301 ctacgcctcg gacggcgaga acgcgtcgga ggaccaggag cccgcggccg cgtcgatgga
     9361 gcgcctcgcc ggaaggctga actacgccgg gtacgccgag atcggcgtca ccggctcgcg
     9421 cctgcggcag acgccgctag gcgcgctgtt cacggatctg caggggcgcg gattcccggc
     9481 cgcgatggcg accatcgcgc ggcaggacga cgtgtggcgc gcgatccgca ctttcttcag
     9541 cgagcaggcg gaggcgacgg cgtgacgcag ctccaggagt acgcctcgaa gatcgaggag
     9601 ctggcgcgcg agctgggcct cgactactac ccggtcgact tcaacgtcac gccgtcgagc
     9661 ttcatgatcg agatcgcggc gtacgggctg ccggtgcgca tgccgcactg gtccttcggc
     9721 gtgcgctaca tccaccagct cgtgcgccag agcatgggcc actcgaagat cttcgaggtg
     9781 atgttccccg gcaacccgtg ctacgcctat ctcatggaca ccaacacggt ggccgagaac
     9841 tcgctcgtca ccgcgcacgt ggtcgggcac gcggacttcg ccaagaacaa ccagctgttc
     9901 gcgcgctacc accgccaggc gggcggcaac atcgtggagc aggcggcggc gcacgcgcac
     9961 cggatcgaat cggcggtctc ggagttcggg ctgcaggccg tcgagtcgac gctcgacgcg
    10021 gcgctcgccc tcgaggcgca cgtcgacatc gaccaggaga tgcaccgcga gcgctatccc
    10081 gagcgcgggc ccgagggggg cgcggcgccg gcggacatgt tccacgagcg cttcaaccgg
    10141 ctgcccgggg aggccgcgcc gcagcaggcg ccgcgccagg cgccgcggct gccggtgccg
    10201 ccgcggcccg agtacgacct gctgtggttc atcgcgcagt acgcgcccga gctcgagcag
    10261 tgggagcgcg atgtcttcct ggcggtgcgc gaggagtcga tgtacttcta cccggtgttc
    10321 gcgtgccaga tcatgaacga gggctgggcc tcgtactggc acgcgcgcct gctgcgcgag
    10381 gcggacttcc tcccgcacaa cctctacctc tcggcgctca agtcgcattc cgacgtggtg
    10441 cggccgttcg cgtccaacca gaacaccgcg ctcgcggtga acccgtacca cctcgggttc
    10501 tcgctctggg agcacctggt gcagacggcc ggcatggaga aggcgcgcga gatctgccgc
    10561 gacgaggacg acttcggctt catccgcaac tatctcgatc gggacctcgc ggacaagctc
    10621 aagctcttcg tgtacgaggc cgacgccgac ggcgagatcc gcgtcgccgg ccgcgacatc
    10681 aaccagctgc gcgaggcgat cctcgcgccg aagttcaact acggcgcgcc gcgcatcgcg
    10741 gtctcggagc tgcgccacga cgggacgctc gtcctcgtgc acgacaacgc gggagacggg
    10801 cgcggcctcg acctcgaccg cgcggcgcgc gtgctgcagt acatccagcg ggtgtggcgg
    10861 cgccccgtgc ggctgcatac ggtcgacgag ttcgggagga accgcgagct ggccgcctga
    10921 cgtagaatcg cgggccaggg cccatacgtc aggagaggat ccatgagatc ggtcgttcgc
    10981 ttgctggccg ccgtcgccgg gacggcattg atcgccgcgc tgttcgcaaa cgaggcgctg
    11041 gcgcagaacg cgcgcaatta tcccgaccgt ccggtcaagc tggtggtcgg ttacgccccg
    11101 ggcgggctgc ccgacaccgt cgcgcgcatc gtcggccagg cgctcggcga gcgctggcat
    11161 cagcagccgg tcgtcgagaa tcgtcccggc gcgaacggca tcgtcggcgc ggaggtggtc
    11221 gtcaagtccg ccgccgacgg ctatacgctg ctggtgacgg acaactccac caccgcgatc
    11281 aacccgttcc tctacaagaa gctgccttac agctcgaagg atctcgcgcc cgtgtccatg
    11341 atcgcgcgcg cgcccctgta tcttgccgcg aacccgtcgt tcccggcgaa ttcattgaag
    11401 gagatgatcg acctggtcaa ggcgaacccg ggcaagtaca gctacggctc gtcgggcatc
    11461 ggcagcacgc accacttgtg ccaggagttc atgaacgccg ccctcgggct gcagatcacg
    11521 cacgtgccgt acaagggcac ggggcagagc gtgccggcgg tcgtcgcggg gcaggtggcg
    11581 atggcgtggt ccgcgggccc gtccctcgcc gcctacgtga aggacgggcg gctgaagctg
    11641 ctcgcggtca actcgatcaa gcgcacgccc gcggcgccga atctgccggc ggtctcggaa
    11701 gtcatccccg gtttcaactt ctcgccgacg atcggcgtgt tcgcgcccgc gggaacgcac
    11761 aaggacgtca tcggcaggat cgcctcggac gtggccgagg cggtgaaggt ccccgaggtg
    11821 gtcacgcgcc tcaccaacct cgacatccag ccgatcggca gcacgcccga ggaatacgcc
    11881 gccgatctgc gggccgacga cgagcgctat tccaaggcgg tgaagatctc cggcgcaaag
    11941 gtcgactgag gagcgccgtg gccgccgtcg tcaagcccga ccgcgcggcc ttctcgcgcg
    12001 agctcgaggc gctcgacgtg cgcccgctgt gggagcgggt gatgcggctc aagcccggaa
    12061 ccgcggcggt tccggcgatg tggcgctggg cgcagttgcg gccgcggctg gcgcgcgcct
    12121 cggagctgat caccgcgaag gaggccgagc ggcgcgtgct gatgctcgag aacccggcgc
    12181 tgcgcgggac caccttcgcc acgaccacgc tctacgcggg gctgcagatc atcatgccgg
    12241 gtgagatcgc cccgacccac cgtcacacgc cgaacgcgct gcgcttcatc gtcgagggcg
    12301 agggcgcgta taccgccgtc gacggcgagc gcgtggcgat gcggcccggc gacttcgtgg
    12361 tcacgccgaa ctggacctgg cacgatcacg gcaacgccgg ctccgagccg g
//
LOCUS       DATDMW010000033         7406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1875420, whole genome shotgun sequence.
ACCESSION   DATDMW010000033 DATDMW010000000
VERSION     DATDMW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875420"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VI319_01125"
     CDS             <1..236
                     /locus_tag="VI319_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6862473.1"
                     /translation="YLEKFYFDTITFDTAMLRHLIDRWGPDHVLLGTDYPFDMGEDDP
                     VGLISRVPGLKPAEREAIMGGTAARLLKLKTSR"
     gene            complement(187..2859)
                     /locus_tag="VI319_01130"
     CDS             complement(187..2859)
                     /locus_tag="VI319_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862474.1"
                     /translation="MLGRSLPLPLAFFASGAAALLFQVLWFRALGRVLGNTVWAGAAV
                     LAGFMLGMALGGLLAARFAPRIRDAARAFALAEATVGLTGSLLVWGLPALEPVAGQWL
                     APLASHPAASAAARFALAVAVMLVPTIAMGTTLALGVRAHARDDTVRSLGALYAANTF
                     GACVAPLLAEYVLIGAFGLRGTALVASGLNLAAAALALAHRRAVAAPPVSHTTSPRRL
                     PARTLLAAAAAGAFALGLEVVWFRLLVLYEPGSDTMFALMLTIVLLGIAAGGAIAPLL
                     ARAGSAWVGAGASLAVVAGYLLSAPDRPDVAAQALNLMLPAAVLSGSFFTLLGAELRG
                     DSGDPQPAIGALMTANTLGAAAGAALAALVLLPRLGIETGVFTLAAGYALLPLLLARP
                     MKRSALPLVAAAVGLLLFPFGRIDGHLSAAARPYEGSDDARVVRITQGPTTTLQLLEH
                     DRFGEPAAWRLLTDGYSMTAIDTRAMRYMRMFAWLPLALHPDPHRALLISYGAGNTAQ
                     ALLSDPGMRALTVVDVSTEILGASPVVHGAQDPLADPRVRLVLEDGRHYLRTRRDQFD
                     IITSEPPPPLIAGVVNLYTREYFTALAQHLRPGGLATYWLPISQFKSVGARAVVRAFC
                     EAFPDCTLWTGVARDWILMGGREFGARPSAEHFSRLWRDAVAGARMAEDGLERPAQLG
                     ATFLADAEQLRRWYEGTPALTDDFPKRIAPDDGIKRPMTEYAAWLRPDDARSRFHESR
                     WIAAHWPTPLAGAADPFFAVQPALNGDIGGDPARSLASVDLLLRGTDLRIPVMWLLGS
                     NVTEQKIVNRKIGLEGRRPEFSYPLAVRALAGRDYALAAKLFAEVAERGNRSAGVLAA
                     YALCRAKRPKPDGACVAAVTAMSSASAASPPSRP"
     gene            complement(2859..3539)
                     /locus_tag="VI319_01135"
     CDS             complement(2859..3539)
                     /locus_tag="VI319_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY6862475.1"
                     /translation="MGSGALCGIALLTVFTAVAQVPPGAGEAGRYRGLHAAAYRGDVA
                     AIQKLLADKADVNARDAYGRTPLHVATFARRRDAIRALVAGGADLGALERDRYDAVTI
                     ASVADDEETLRLLLSLGASAKLVTSRYDGTALIAAAHLGHDGVVRQLIAAGAPLDHVN
                     NLHWTAVIESIVLGDGGPRHVASLRALVDAGANLQLADRGGNTPLQLAKSRGYRQMAE
                     MLEKAGAR"
     gene            complement(3563..4189)
                     /locus_tag="VI319_01140"
     CDS             complement(3563..4189)
                     /locus_tag="VI319_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY6862476.1"
                     /translation="MRCEHADALSDGRLVSAAAERNRGPILEVLERVLPAAGLVLEIA
                     SGTGQHVIHFAAALTGLAWQPSDPDADCRRSIRAWLSAAKLANVREPLDLDVSTAPWP
                     IAAPDAIVCLNMIHIAPWSATGSLFAGAATALKAGGLVFLYGPYFVPGEDPAPGNLAF
                     DRTLRARNPEWGVRNLQDVSSIARESGFDRIETVAMPANNLSVVFRRG"
     gene            complement(4204..5118)
                     /locus_tag="VI319_01145"
     CDS             complement(4204..5118)
                     /locus_tag="VI319_01145"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6862477.1"
                     /translation="MAQPAVGMIGLGIMGSAMSYNLLRAGFPVVGYDVSDQARKDHEK
                     AGGKVAKSVADVAKRAETLITSLPSYKALTTVAEELGSATSKSHVVIETSTMPIAIKE
                     AARDAVARSGAVLLDCPLSGTGAQARAKDLLIYVSGERKAYDRVAKVMEGFTSGQYYI
                     GPFGDGSKMKFVANLLVAIHNVAAAEAMVLGMKAGLDPAKVLEVVGGGAGGSRMFQVR
                     GPMMVKGDYSEATMKVEVWQKDMTIIGDFVRELDSPAPLFAASGPIYNAAMAMGLAKE
                     DTGAVCKVLEEMAGSPRRAGRAKTGPAR"
     gene            complement(5205..5789)
                     /locus_tag="VI319_01150"
     CDS             complement(5205..5789)
                     /locus_tag="VI319_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6862478.1"
                     /translation="MPRPSPWDERAATLRRVSGHLEKIFHELTGGRLRGMVPRVPGAW
                     LPQVAVFERGDRLVVSVDLPGIRREDVEVDIDDRAITIRGERHPTGARADTRAECSYG
                     RFLRSVPLPEGADAGAARAVIRDGVLEITLPLPPKRQPRRLEIEDAGTAARKDTYRNQ
                     TAEPANEPHRSETGGSEHDRRERRDWQSEGRLGM"
     gene            complement(5796..6269)
                     /locus_tag="VI319_01155"
     CDS             complement(5796..6269)
                     /locus_tag="VI319_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY6862479.1"
                     /translation="MAHIEKSIDVNCPVRAAYNQWTQFEDFPRFMEGVKEVHQLDDTH
                     LHWHAEIGGKDKEWDTVITEQVPDSIIAWRSVAGAENAGRVDFQPDQSGNATHITLKL
                     TYEPEGAVEKIGDWIGVVSRRVEGDLKRFKEFVESRGRETGQWRGEVHRGRKLSS"
     gene            6510..>7406
                     /locus_tag="VI319_01160"
     CDS             6510..>7406
                     /locus_tag="VI319_01160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6862480.1"
                     /translation="MHAPPRPAVSVVVPTCGRPGLLDRCLRALAAQSMAAAQFEIVVV
                     DDRPSAATERAVADWAQRAHPRDIRYLASPGPHGPAAARNRGWRAARGDIVAFTDDDT
                     APDPDWLCNGLHAMRGDVDAACGRIVMPIPERPTDYERDAQGLERSEFVTANCFCRRR
                     VLEALGGFDERFGLAWREDSDLHFRLIESGARIARAQDAVVVHPVRPAPWGVSMRQQR
                     KVAYDALLYKKHPRLYRERIRARPRWDYYVAVAALLALALGLAAGEPRVAAGAGLVWL
                     ALTARFCAARLRGASKAPAHVLE"
BASE COUNT         1058 a         2662 c         2639 g         1047 t
ORIGIN      
        1 gctatctgga gaagttctac ttcgacacga tcaccttcga caccgcgatg ctgcgccacc
       61 tgatcgaccg ctgggggccg gaccacgtgc tgctcgggac cgactatccc ttcgacatgg
      121 gcgaggacga cccggtggga ctgatctcgc gcgtgccggg cctgaagccc gcggagcgcg
      181 aggcgatcat gggcgggacg gcggcgaggc tgctgaagct gaagacatcg cggtgacggc
      241 ggcgacgcac gcgccgtcgg gcttcggccg cttcgcgcgg catagcgcgt acgcggcgag
      301 gacaccggcg gaccggttgc cgcgctccgc gacctcggcg aacagcttcg cggcgagcgc
      361 atagtcgcgc ccggcgaggg cgcgcacggc gaggggatag ctaaactccg ggcgccggcc
      421 ctccagcccg atcttccggt tcacgatctt ctgctcggtg acgttgctgc cgagcagcca
      481 catcaccggt atgcgcaggt cggtcccgcg taggagcaga tccacgctcg caaggctgcg
      541 ggccggatcg ccgccgatat cgccgttcaa cgccggctgc actgcgaaaa acgggtcggc
      601 cgcaccggcg agcggcgtcg gccagtgcgc ggcgatccag cgcgattcgt gaaagcgact
      661 gcgcgcgtcg tcgggcctga gccaggccgc gtactcggtc atcggccgct tgatgccgtc
      721 gtcgggcgcg atgcgcttgg ggaaatcgtc ggtcagcgcg ggcgtgcctt cgtaccagcg
      781 gcgcagctgc tcggcgtcgg cgaggaacgt cgcgccgagc tgcgcggggc gctcgagtcc
      841 gtcctccgcc atgcgcgcac ccgccacggc gtcgcgccag agccgcgaga aatgctccgc
      901 gctcggcctc gcgccgaact cgcgcccgcc catcaggatc cagtcgcgcg cgacccccgt
      961 ccacagggtg cagtccggaa acgcctcgca gaaagcgcgc accaccgcgc gcgcgccgac
     1021 cgacttgaac tgcgatatcg gcagccaata ggtcgccaga ccgccggggc gaaggtgctg
     1081 cgccagcgcc gtgaaatact cgcgcgtata gaggttcacc acgcccgcga tcagcggcgg
     1141 tggcggctcc gaggtaatga tgtcgaactg gtcgcgccgg gtgcgcagat agtgccgccc
     1201 gtcttcgagc acgagccgga cgcggggatc ggcgagcggg tcctgcgcgc cgtggacgac
     1261 cgggctcgcg cccaggatct cggtcgagac gtcgaccacg gtcagcgcgc gcatgccggg
     1321 atcgctcaag agcgcttgcg ccgtgttgcc cgcgccgtag ctgatcagca gcgcgcgatg
     1381 cgggtccgga tgcagcgcga gcggaagcca cgcgaacatc cgcatgtatc gcatcgcgcg
     1441 cgtgtcgatc gcggtcatcg agtagccgtc ggtcagcagc cgccacgccg ccggctcgcc
     1501 gaagcggtcg tgctcgagca gctgcagcgt cgtcgtcggc ccctgcgtga tgcgcaccac
     1561 ccgcgcgtcg tcgctgccct cgtagggtcg cgcggcggcc gagagatggc cgtcgatgcg
     1621 gccgaacggg aacagcagca acccgaccgc cgcggcgacg agcggcagcg ccgatcgctt
     1681 catcggccgc gcgagaagca gcggcagcag cgcgtatccg gcagccagcg tgaacacgcc
     1741 cgtctcgatc ccgaggcgcg gcagcagcac cagcgcggca agcgcggcgc cggccgcggc
     1801 gccgagcgtg ttcgcggtca tcagcgcgcc gatcgccggc tgcggatcgc cggaatcgcc
     1861 gcgcagctcg gccccgagca gcgtgaagaa cgacccggag agcaccgccg cgggcagcat
     1921 taggttcagc gcctgcgccg cgacatcggg ccggtccggg gccgagagga gatagccggc
     1981 gaccaccgcg aggctggcgc ccgctccgac ccacgcgctg cccgcgcgcg cgagcagcgg
     2041 ggcgatcgcc ccgccggccg cgattccgag caggacgatc gtgagcatga gcgcaaacat
     2101 cgtgtcgctc cccggctcgt acaggaccag caggcggaac cagacgacct ccaggccaag
     2161 cgcgaaggcg ccggcggcgg cggcggcgag aagcgtgcgc gccgggagac ggcgcggcga
     2221 cgtcgtgtgc gacacgggcg gggccgcgac ggccctgcgg tgcgcgagcg caagcgccgc
     2281 ggcggcgagg ttgaggccgg aggcgaccag cgccgtgccg cgcaggccga aggcgccgat
     2341 caggacgtac tcggcgagca gcggcgcgac gcaggcgccg aacgtgttcg cggcgtacag
     2401 ggcgccgagc gagcgcacgg tgtcgtcgcg cgcgtgcgcg cgcacgccca gcgcgagcgt
     2461 cgtgcccatc gcgatcgtcg gcacgagcat gactgcgacc gcgagggcga agcgcgccgc
     2521 ggcgctcgcc gcggggtgcg acgcgagcgg agccagccac tgccccgcga ccggctcgag
     2581 cgcgggcagg ccccagacga gcaggctccc ggtgagtccg accgtcgctt ccgccagcgc
     2641 aaaggcgcga gcggcgtcgc ggatccgcgg cgcgaagcgc gcggcgagca acccgccgag
     2701 tgccatgccg agcatgaacc cggcgagcac cgccgcgccc gcccagacgg tgttcccgag
     2761 aacgcgaccc agcgcgcgga accacagcac ctggaacaga agcgccgcgg cgcccgaggc
     2821 gaagaacgcg agcggcagtg gcagcgatct tccgagcatc agcgcgcgcc cgccttctcg
     2881 agcatctcgg ccatctgccg ataaccgcgc gacttcgcga gctgcaaggg cgtattgccg
     2941 ccgcggtcgg cgagctgcag attggcgccg gcgtcgacga gcgcgcgcag cgaggcgacg
     3001 tggcgcgggc cgccgtcgcc cagcacgatc gattcgatga ccgcggtcca gtggaggttg
     3061 ttgacgtgat cgagcggcgc ccccgccgcg atgagctggc gcaccactcc gtcgtggccg
     3121 aggtgcgcgg cggcgatcaa cgccgtgccg tcgtagcggc tggtgacgag cttcgcgctc
     3181 gcgccgagcg agagcagcag gcgcagcgtc tcttcgtcgt cggcgaccga cgcgatggtg
     3241 accgcatcgt agcggtcgcg ctcgagcgcg ccgaggtccg cgccgcccgc gacgagcgcg
     3301 cgtatcgcgt cgcgccggcg cgcgaacgtc gcgacgtgga gcggggtgcg gccgtaggcg
     3361 tcgcgggcgt tcacgtcggc tttgtccgcg agcagcttct ggatggcggc gacgtcgccg
     3421 cggtaggcgg cggcgtggag tccgcgatac cgccccgctt ccccggcgcc cggcgggacc
     3481 tgcgccacgg cggtgaaaac ggtcaggagc gcgataccgc agagcgcccc cgatccgatg
     3541 cgcctgacag atggcattgt tttcatcccc ttctgaacac cacgctcaga ttgttcgcgg
     3601 gcatcgcgac ggtctcgatc cggtcgaagc cggattcacg tgcgatcgac gacacgtcct
     3661 gcaggttgcg cacgccccat tccgggttgc gtgcacgcag cgtgcgatcg aaagcgaggt
     3721 tgcccggcgc ggggtcttcg cccgggacga agtaaggacc gtacaggaac accagcccgc
     3781 ctgccttcag cgcggtcgcc gcgcctgcga acagcgaacc tgtcgccgac cacggcgcga
     3841 tgtggatcat gttcaggcag acgatcgcat ccggcgccgc tatcggccag ggcgcggtac
     3901 tcacgtccag atcgagcggc tcgcgcacgt tggcgagctt cgcggcgctc agccaggcgc
     3961 ggatggatcg gcggcagtcg gcatccggat cgctcggctg ccaggcgagt ccggtcagcg
     4021 ccgccgcgaa atggatcacg tgctgcccgg tcccgctggc gatctcgagc accagtcccg
     4081 ccgcggggag gacgcgctcg agcacctcga gtatcggccc gcggttgcgc tcggccgccg
     4141 ccgacaccag ccggccgtcg gagagcgcgt ccgcgtgttc gcagcgaatc gcaggctccc
     4201 ggatcagcgg gcgggtccgg tcttcgcgcg accggcacgc ctcggcgatc cggccatctc
     4261 ctcgagcacc ttgcacaccg cgcccgtgtc ctccttcgca aggcccatcg ccatcgccgc
     4321 gttgtagatc gggccgctcg ccgcgaacag cggcgccggc gagtcgagct cgcgcacgaa
     4381 atcgccgatg atggtcatgt ccttctgcca gacctcgacc ttcatcgtcg cctcggagta
     4441 gtcgcctttc accatcatgg ggccgcgcac ctggaacatg cgcgagccgc cggcgccgcc
     4501 gccgacgacc tcgagcacct tggcgggatc gagcccggcc ttcatgccga gcaccatcgc
     4561 ctcggcggcg gcgacgttgt ggatggcgac gaggaggttg gcgacgaact tcatcttcga
     4621 gccgtcgccg aaggggccga tgtagtactg cccgctcgtg aagccttcca tcaccttggc
     4681 gacgcggtcg taggccttgc gctcgccgct cacgtagatg aggagatctt tcgcgcgcgc
     4741 ctgggcgccg gtgccgctca aagggcagtc gaggagcacg gcgcccgatc tcgcgacggc
     4801 gtcgcgcgcc gcctccttga tcgcgatcgg catggtgctg gtctcgatca cgacgtggct
     4861 cttgctggtg gccgagccga gctcttcggc gacggtggtc agcgccttgt acgacggcag
     4921 ggacgtgatg agggtctccg cgcgcttcgc cacgtcggcg acgctcttcg cgaccttgcc
     4981 gcccgccttc tcgtggtcct tgcgcgcctg gtccgaaacg tcgtagccga cgacgggaaa
     5041 tcccgcgcgc agcaggttgt acgacatcgc cgagcccatg atgccgagcc cgatcatgcc
     5101 gacggccggt tgggccatgg ggacgctcct cttcgtgtgc gggaagctgc tattttgccc
     5161 gaacggcgcg ggcggtcgcg ctcgaaaagt gccccgcgta agcgttacat ccccaggcgc
     5221 ccttcgctct gccaatcgcg gcgctcgcgg cggtcgtgct cactgccgcc cgtctctgag
     5281 cgatgcggct cgttcgcggg ctcggcggtc tggttgcggt aggtgtcctt gcgcgccgcg
     5341 gtgccggcgt cctcgatctc gagccggcgc ggctggcgct tcggcggcag cggcagcgtg
     5401 atctcgagca cgccgtcgcg aatcacggca cgcgcggcgc ccgcgtccgc gccctcgggt
     5461 agcgggaccg agcgcaggaa gcgcccgtag ctgcactcgg cccgcgtgtc cgcgcgggcg
     5521 cccgtcgggt ggcgctcgcc gcggatcgtg atcgcgcgat cgtcgatgtc gacctcgacg
     5581 tcctcgcggc ggatccccgg aagatcgacg ctgaccacga gccggtcgcc gcgctcgaac
     5641 accgcaacct gcggcagcca cgcgcccggg acgcgcggca ccatcccgcg cagccggccg
     5701 ccggtgagct cgtggaagat cttctccagg tgccccgaga cgcggcgcag cgtcgccgcc
     5761 cgctcgtccc agggagacgg acgcggcatg gccggctagc tcgagagctt ccggccgcgg
     5821 tgcacctcgc cgcgccactg cccggtctcg cggccgcggc tctcgacgaa ttccttgaag
     5881 cgcttcagat cgccttcgac gcgtcgcgac accaccccga tccagtcgcc gatcttctcg
     5941 accgcaccct cgggctcgta ggtgagcttc agcgtgatgt gcgtggcgtt gccgctctgg
     6001 tcgggctgga agtcgacgcg gccggcgttc tccgcgccgg cgacgctgcg ccaggcgatg
     6061 atcgagtcgg gcacctgctc ggtgatcacc gtgtcccact ccttgtcctt gccgccgatc
     6121 tcggcgtgcc aatgcagatg cgtgtcgtcg agctggtgca cttccttcac gccttccatg
     6181 aagcgcggga agtcctcgaa ctgcgtccac tggttgtaag cggcgcgcac cgggcaattc
     6241 acgtcgatcg atttctcgat atgggccatg aaatttcctt tcgatgaagg gcattcactg
     6301 cggacgcaaa ttccgtaccg atcgccgccg cgcgcgcatc ccccgtgggc gccgaaggtg
     6361 cacgcccgaa cggcacgatt tacagcgcgt ccccgtaaga attacagcgt ccgctcggcg
     6421 tccggcgcgc gcgccgcggt cggcgtttcg tggaacagga gttgctttat gtcgctcgaa
     6481 tgccgctcta tgcatcgaac gacgcacgca tgcatgcgcc tccccggccg gcggtgtcgg
     6541 tggtcgttcc cacctgcggt cgccccgggc ttctcgacag gtgcttgcgc gcgctcgccg
     6601 cgcagagcat ggctgcggcg cagttcgaga tcgtcgtggt cgacgaccgg ccctccgcgg
     6661 cgaccgagcg cgccgtagcg gactgggcgc agcgggccca cccgcgcgac atccgctacc
     6721 tcgcctcgcc cggaccgcac ggccccgcgg ccgcgcgcaa ccgcgggtgg cgcgcggcgc
     6781 gcggcgacat cgtcgccttc accgacgacg acaccgcgcc cgatccggac tggctgtgca
     6841 acggcctgca cgcgatgcgc ggcgacgtcg acgcggcatg cgggcgcatc gtcatgccga
     6901 ttcccgagcg tcctaccgac tacgagcgcg acgcgcaggg gctcgagcgc agcgagttcg
     6961 tgacggcgaa ctgcttctgc cgccggcgcg tgctcgaggc gctcggggga ttcgacgagc
     7021 gcttcggtct cgcgtggcgc gaggattccg acctgcactt tcgcctgata gagtccggag
     7081 cgcgcatcgc gcgcgcgcag gacgcggtgg tcgtgcatcc cgtgcgtccc gcgccctggg
     7141 gcgtgagcat gcggcagcag aggaaagtcg cctacgacgc gctcctctac aagaagcatc
     7201 cgcgcctgta ccgcgagcgc atccgcgcgc ggccgcgctg ggactactac gtcgctgtgg
     7261 cggcgctgct cgcgctcgcg ctgggcctcg ccgcgggcga gccgcgcgtc gccgcgggcg
     7321 ccggcctcgt atggctcgcg ctcaccgcgc gcttctgcgc cgcgcgcctg cgcggcgcgt
     7381 cgaaggcccc cgcgcacgtg ctcgag
//
LOCUS       DATDMW010000034         7564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_755189, whole genome shotgun sequence.
ACCESSION   DATDMW010000034 DATDMW010000000
VERSION     DATDMW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755189"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="VI319_01165"
     CDS             <1..1547
                     /locus_tag="VI319_01165"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6862481.1"
                     /translation="ADVCESQGRLDAAGAALETALRARPDWTGALFNHARVLYRQERP
                     AEAEAALRRLVSIDPADASAWGLLGDVLRARVRVPEALEALARARSLAPDRFDLQSAE
                     LFMLQSSEGASAEGLFERHRQFGARLEAAIGARFAPFANAPDPERRLRVGYVSGDFHT
                     HPVAFFMIPLLERHDRERVRTFCYSTGRLHDRITEEVRARAHEWRDASWMPDAELADA
                     VNGDGIDILVDLSGHSGVSRLPVFAQQPAPVQVAWLGYLATTGLTRMQYRLCDRYTDP
                     PGAAERFHTEKLVRLPACQWCYRPPVGAVPCADVPPFVRNGFLTFGSFNAALKISPSI
                     RRLWARILERVPGSRLKLVGIPQESTRREIVADLAAGGIAPDRISASPRLGLLEYFEA
                     FAGVDVALDPAPYSGGTTTCDTLWMGVPVVTAPGERSISRSAGSILHTLGLDDWIAAS
                     PEDYVERAVRCDRERERLVALRRDLRETLRASPLMDEPRFARDIEAAYRGMWRAWCRA
                     RGAPST"
     gene            1557..3800
                     /locus_tag="VI319_01170"
     CDS             1557..3800
                     /locus_tag="VI319_01170"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6862482.1"
                     /translation="MFERLLERLLRRAATLSARAGAPADDRSAAERCIEAGRAAEREG
                     RTADACGHYREAVAAAPGYAKARLELGIGLEATGDADGALAAYREAVDLDAADPYASY
                     NLGRLLQARGELREAERLVRAALARKADFVEARIVLAGIHEIGGALAAAAGELEIVLR
                     HRPEHPGALLNYGLVLAKQDRTAEAESALRRAVAADPRSASANQRLASMLLERGETAE
                     AERLFEAALEHEPALVEAHVRLFELCRKRGDIAAAAARLARLLEHRPDWADAWYDYGL
                     MLKKLFKRAEAEAAFRRAVALDPRHVGAQRMLGGMLLSQARVEEAIASYELALQHNPE
                     QFEIESAEMFALLFVEDTPDEELFARHKALGARIEAAFPPRYAAFGNAREPERRLRIG
                     YVSGDFYTHPVAFFLLPLLERHDRSAFEIHAYSVGDNVDDVTRRVSALVDGWHDCAAL
                     SAHELADAIHRDAIDVLVDLSGHSGVSALATFALQPAPVQATWLGYLATTGMTRMHWR
                     LCDRHTDPPGATERFHTEALARLPNSQWCYRPVFTEAPAEPPLARNGFATFGSFNQGA
                     KLSPSTRALWAEILGRVPGSRLAVLDIEGPGADAFVADLSRRGVDPARVTIVPRVPPA
                     DYFRWFNRVDVALDTTPYSGGTTTCDTLWMGVPIVTLPGSRPASRSTASLLTTVGAGE
                     WIAATPADYVRLAVELAARAPAGAEARLALRERMRGSAIMDEPRFARDVEAAYRGMWR
                     AWCARGA"
     gene            3839..4171
                     /locus_tag="VI319_01175"
     CDS             3839..4171
                     /locus_tag="VI319_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862483.1"
                     /translation="MFRSWFDCKEVDAFVDGIVAELAKRFPPSGVELPAKKAIERLRR
                     TNESILRQVESFSLSHALNLYKKAHLGNRFKWALKEAGYPGEFVDALTYELVTVVALA
                     SRRRKPAA"
     gene            complement(4187..5308)
                     /locus_tag="VI319_01180"
     CDS             complement(4187..5308)
                     /locus_tag="VI319_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY6862484.1"
                     /translation="MLRISINTKWALEARDETAHSLDRTLKLLAAIKDEGNLYRGARR
                     IGLSYRHAWGTIRDAGVLFGAPVLKMSRGRGAVLTPLGEKLLWADKRVWARLAPVLDS
                     IASELEAEIERTVSEDAPILRIHASHGFAIGLLRDFLSRSHVSVDLKYRGSMEALASL
                     AAGDCDMAGFHVPQGELQGEVLKFYSKWLRPEGHTLVNLATRRQGIILAPGNPKRIHS
                     LADLARPDVRFVNRQLGSGTRILLDLLLKRADVESREIRGYDTGESTHSGVAAYIASG
                     LADAGFGVETAARAFKLDFIPVAAARYFLICEDDALTSPVVTRILEVLTSGAFRAAAG
                     KLVGIDVTYAGTVLRIPDAFPELGAVRARRAKRETARSR"
     gene            5476..7143
                     /locus_tag="VI319_01185"
     CDS             5476..7143
                     /locus_tag="VI319_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEY6862485.1"
                     /translation="MSEAAVYTGNGTSGVGLLAKERIIAGPGFNRWLVPPAALCIHLC
                     IGMAYGFSVFWLPLSRAINAKQSIACPADMSFIARMFSTTCDWRISELGWMYTLYFVF
                     LGLAAAIWGGWLERAGPRKAGFVAACCWCGGLVFSAIGVYLHQIWMMWLGTGIVGGVG
                     LGLGYISPVSTLIKWFPDRRGMATGMAIMGFGGGAMIGAPLADILMRHYATATSVGVV
                     ETFITMAIIYFVFMTAGAFGYRIPAPGWKPEGWTPPAPSAKSMMTARHVHVRQAPKTP
                     QFWLIWWVLCLNVTAGIDIIGMASPMLQEVFAGKLIGINVAFNDLTAVQRGQIAAIAA
                     GFTGLLSLFNIGGRFVWASLSDFIGRKTTYYTFFVLGIALYASAPWSGHLGHISLFVG
                     AFCIILSMYGGGFSTVPAYLADMFGTQMVGAIHGLILTAWSAAGIFGPVIVNYMREFQ
                     LAHGVPRAQVYDTTAYILCGLLAIGFICNLLIKPVADKHFMTDAELDAERKLAHEASR
                     AATASSRGPADSAKTPTAIVVGAWLVVGIPIIWGVWVTLEKAWVLFR"
     gene            7255..>7564
                     /locus_tag="VI319_01190"
     CDS             7255..>7564
                     /locus_tag="VI319_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY6862486.1"
                     /translation="MNDRAAPPDSPTRKPGKGPRVRATPKGRIVDPKAREEVLTLLGA
                     NPRRRDLLIEYLHRIQDRYGHLAAPHLVALAAELKMAMTEVYEVATFYHHFDVVKEGE
                     T"
BASE COUNT         1065 a         2671 c         2653 g         1175 t
ORIGIN      
        1 tcgcggacgt gtgcgagtcg cagggccggc tcgacgcggc gggggcggct ctcgaaaccg
       61 cgttgcgcgc gcggcccgac tggaccggcg cgttgttcaa tcacgcccgg gtcctgtacc
      121 ggcaggagcg gccggccgag gccgaggcgg cgctccgccg cctcgtctcg atcgacccgg
      181 cggacgcctc ggcctgggga ctcctgggcg acgtgctgcg cgcgcgggtg cgcgtgcccg
      241 aggcgctcga ggcgctcgcg cgcgcgcgct cgctcgctcc ggatcgcttc gatctgcagt
      301 cggccgagct gttcatgctc cagtcgtcgg aaggcgcgag cgccgagggc ctgttcgagc
      361 ggcaccggca gttcggcgcg cggctcgagg cggcgatcgg cgcgcgcttc gcgcctttcg
      421 cgaacgctcc cgaccccgag cggcggctgc gcgtcggcta cgtctcgggc gacttccata
      481 cgcacccggt cgcgttcttc atgatcccgc tgctcgagcg gcacgatcgc gagcgcgtcc
      541 gcacgttctg ctattcgacc ggccggctgc acgaccgcat cacggaagaa gtccgggcac
      601 gcgcgcatga atggcgcgac gcatcgtgga tgcccgacgc cgagctcgcc gacgcggtga
      661 acggggacgg tatcgacatc ctcgtcgacc tgtcggggca cagcggcgtt tcgcgcctgc
      721 cggtgttcgc gcagcagccg gccccggtgc aggtcgcgtg gctcgggtat ctcgcgacga
      781 cgggactgac gcggatgcag taccgcctgt gcgatcgata caccgatccg cccggcgccg
      841 ccgagcgctt ccacaccgag aagctcgtgc ggctgccggc ctgccagtgg tgctaccgtc
      901 cgccggtcgg cgcggtgcct tgcgccgacg tcccgccgtt cgtgcgcaac ggcttcctca
      961 cgttcggatc gttcaacgcc gcgctgaaga tctcgccgtc catacgccgg ctctgggccc
     1021 ggatcctcga gcgcgtgccg gggtcgcgcc tgaagctggt cggcattccg caggagagca
     1081 cgcgccgcga gatcgtcgcc gatctcgccg ccggcggaat cgcgccggac cgcatcagcg
     1141 cctcgccccg gctcgggctg ctcgagtatt tcgaggcgtt cgccggggtc gacgtcgcgc
     1201 tcgatcccgc gccatacagc ggcggcacga ccacctgcga cacgctgtgg atgggcgtgc
     1261 cggtcgtcac cgcgccgggg gagcgctcga tctcgcgcag cgcgggaagc atcctgcaca
     1321 ccctcgggct cgacgactgg atcgcggcct cgcccgagga ctacgtcgag cgcgcggtgc
     1381 gttgcgaccg cgagcgcgag cggctggtcg cgttgcgccg cgacctgcgc gagaccttgc
     1441 gcgcgtcgcc gctcatggac gagccgcggt tcgcgcggga cattgaggcc gcgtaccgcg
     1501 gaatgtggcg cgcgtggtgc cgcgcgcgcg gcgcgccatc gacataagga cgacccgtgt
     1561 tcgaacgatt gctcgaacgg ctcctgcggc gcgcggccac gctctcggcg cgcgcgggcg
     1621 cgcccgcgga cgaccgatcg gccgccgagc gctgcatcga agccggcaga gccgccgagc
     1681 gggaagggag gaccgccgac gcgtgcgggc attatcgcga ggcggtcgcg gcggcgcccg
     1741 gctacgccaa ggcacgcctc gagctgggga tcggcctcga ggcgaccggg gacgcggacg
     1801 gcgcgctggc ggcgtaccgc gaggcggtgg acctcgatgc ggccgacccg tacgcgagct
     1861 acaacctcgg ccggctgctg caggcccgtg gagagctgcg cgaagccgag cgcctcgtgc
     1921 gcgcggcgct cgcgcgcaag gcggatttcg tcgaggcgcg catcgtgctc gcgggcatcc
     1981 acgagatcgg cggcgcgctc gccgccgccg cgggcgagct cgagatcgtc ctgcgccacc
     2041 gtcccgagca tcccggtgcg ctcctcaact acggcctggt gctcgccaag caggaccgga
     2101 ccgccgaagc ggagagcgcg ctccggcgcg ccgtggcggc cgatccgcgc agcgcgagcg
     2161 ccaatcagcg gctcgcctcg atgctgctcg agcgcggcga aaccgccgag gccgagcgcc
     2221 tgttcgaggc ggcgctcgag cacgagccgg cgctggtcga ggcgcacgtg cggctgttcg
     2281 agctgtgccg caagcgcggc gacatcgcgg ccgccgcggc ccggctcgcg cgcctgctcg
     2341 agcaccgccc cgactgggcg gacgcttggt acgactacgg cctgatgctg aagaagctgt
     2401 tcaagcgcgc cgaagccgag gccgccttcc gccgcgccgt cgccctcgat ccgcggcacg
     2461 tgggcgcgca gcgcatgctg ggcggcatgc tgctctcgca ggcgcgcgtc gaggaggcca
     2521 tcgcgagcta tgagctcgcc ctgcagcaca acccggagca gttcgagatc gaatccgccg
     2581 agatgttcgc gctgctgttc gtggaggaca cgccggacga ggagctgttc gcgcggcaca
     2641 aggcgctcgg cgcgcgcatc gaggccgcgt tcccgccgcg gtacgcggcg ttcggcaacg
     2701 cgcgcgaacc ggagcgccgg ctgcgcatcg gctacgtctc gggcgacttc tatacgcacc
     2761 cggtcgcgtt cttcctgctg ccgctgctcg agcggcacga ccggtcggcc ttcgagatcc
     2821 acgcgtattc ggtcggcgac aacgtcgacg acgtgacccg ccgagtctcg gccctggtcg
     2881 acggctggca cgactgcgcc gcgctctcgg cgcacgagct cgccgacgcg atccaccgcg
     2941 acgcgatcga cgttctggtc gacctgtcgg gccattccgg cgtctcggcc ctcgccacct
     3001 tcgcgctgca gcccgcgccg gtgcaggcga cctggctcgg ctacctcgcg acgaccggca
     3061 tgacgcgcat gcactggcgc ctttgcgacc gacataccga tccgccgggc gccaccgagc
     3121 gcttccacac cgaggcgctg gcgcggctgc cgaacagcca gtggtgctac cggcccgtgt
     3181 ttacggaggc gccggcggag ccgcccctcg cgcgcaacgg gttcgcgact ttcggctcct
     3241 tcaaccaggg agcgaagctc tcgccgtcga cgcgcgcgct gtgggccgag atcctcggcc
     3301 gcgtgccggg ctcgcgcctc gcggtgctcg acatcgaagg gcccggcgcg gacgccttcg
     3361 tcgccgacct gtcgcggcgc ggggtcgatc cggcccgggt gacgatcgtc ccgcgcgttc
     3421 cgccggcgga ctacttccgc tggttcaacc gggtggacgt cgcgctcgac accacgccct
     3481 acagcggcgg caccacgacc tgcgacacgc tgtggatggg cgtgccgatc gtgacgctcc
     3541 cgggatcgcg cccggcgtcg cgcagcaccg cgagcctcct gacgaccgtc ggggcgggcg
     3601 aatggatcgc cgccacgccg gccgattacg tgcgcctcgc ggtcgagctc gcggcacggg
     3661 cgccggccgg cgcggaggcg cggctcgcgc tgcgcgagag gatgcgcggc tcggcgatca
     3721 tggacgagcc gcggttcgcg cgcgacgtgg aggccgcgta ccgcggcatg tggcgcgcct
     3781 ggtgcgcgcg cggcgcctga agcatgcgat aattccgcgc cactttccgg gtgcctccat
     3841 gttccgcagc tggttcgatt gcaaggaggt cgacgctttc gtcgacggca tcgtcgccga
     3901 gctggccaag cgctttccgc cctccggcgt cgagctcccc gcgaagaagg cgatcgagcg
     3961 cctgcgccgg accaacgaat cgatcctgcg ccaggtggag agcttctcgc tctcgcacgc
     4021 gctcaacctg tacaagaagg cgcacctcgg caaccgcttc aagtgggcgc tgaaggaagc
     4081 gggctacccc ggcgagttcg tcgacgcgct cacctacgag ctcgtcacgg tggtggcgct
     4141 ggcgtcccgg cgccgcaagc ccgccgcctg agggctcggg ccgcgctcac ctcgagcgcg
     4201 cggtctcgcg cttcgcgcgt ctcgccctga ccgcgccgag ctccgggaag gcgtcgggaa
     4261 tccgcagcac cgttcccgcg taggtgacgt cgatgccgac cagcttgccg gccgcggcac
     4321 ggaacgcgcc gctcgtcagg acctcgagaa ttcgtgtgac aaccggggac gtgagcgcat
     4381 cgtcctcgca gatcaggaag tagcgcgccg cggccaccgg tatgaaatcg agcttgaacg
     4441 cgcgcgcggc ggtctcgact ccgaaccccg cgtccgcgag accgctcgcg atgtaggcgg
     4501 ccacccccga gtgcgtggac tcgcccgtgt catagccacg gatctcgcgg ctctcgacgt
     4561 cggcgcgctt gagcagcagg tcgagcagga tgcgcgtgcc cgagccgagc tgccggttga
     4621 cgaaacgtac gtcgggacgc gcgaggtccg ccagcgagtg gatccgcttc ggattgccgg
     4681 gcgcgaggat gatgccctgg cgccgggtcg ccagattgac cagggtgtgg ccctccggac
     4741 gcagccactt cgagtagaac ttgaggacct ctccctgcag ctcgccttgc ggcacgtgga
     4801 agcccgccat gtcgcaatcc ccggccgcga gcgacgccag agcctccatg ctgccgcggt
     4861 atttcaggtc gaccgacacg tggctgcgcg aaaggaaatc gcgcagcagc ccgatcgcga
     4921 acccgtggct ggcgtggatg cgcaggatcg gcgcatcctc ggacaccgtc cgctcgatct
     4981 cggcctcgag ctccgaggcg atgctgtcca gcaccggcgc gagcctcgcc cacacccgct
     5041 tgtcggccca gagcagtttc tctcccaacg gagtgagcac tgcgccgcga ccccggctca
     5101 tcttgagcac cggcgcgccg aagaggacgc cggcgtcgcg aatcgtgccc cacgcgtgcc
     5161 ggtacgacag gccgatcctg cgcgcgcccc ggtacaggtt gccctcgtcc ttgatggccg
     5221 cgaggagctt cagcgtgcgg tcgagcgaat gcgccgtctc gtcgcgcgcc tcgagcgccc
     5281 acttcgtatt gatggagatt ctgagcattg gcatccggat tatgttttga attgcatatt
     5341 agatgcgtat cgcggttcat attgttcccg cggatgaacg gatgttacgt tcggcggagg
     5401 attccgccaa gaggaaaggc ggatccacac taagacagat cgatcgacgc aacacggagc
     5461 gaaggaggag ggacgatgtc ggaagcagcg gtatacacgg gcaacggcac cagcggtgtc
     5521 ggtctgctcg ccaaggaacg gatcatcgcc gggcccggct tcaatcggtg gctggtgccg
     5581 ccggcggcgc tgtgcatcca tctttgcatc ggcatggcgt acggcttcag cgtgttctgg
     5641 ctgccgctgt cgcgtgcgat caacgccaag cagtcgatcg cgtgtccagc cgacatgtcg
     5701 ttcatcgcgc gcatgttctc gaccacgtgc gactggagga tcagcgagct cggctggatg
     5761 tacacgctct acttcgtgtt cctcggcctg gcggcggcga tctggggcgg ctggctcgag
     5821 cgggccggcc cgcgcaaggc gggcttcgtg gcggcgtgct gctggtgcgg cggcctcgtg
     5881 ttctccgcga tcggcgtcta cctgcatcag atctggatga tgtggctcgg caccggcatc
     5941 gtcggcggcg tcggcctggg cctcggctac atctcgccgg tgtcgaccct gatcaagtgg
     6001 ttccccgacc gccgcggcat ggcgaccggc atggcgatca tgggcttcgg cggcggcgcg
     6061 atgatcggcg ccccgctcgc cgacatcctg atgcggcact acgcgacggc gacctcggtc
     6121 ggcgtcgtcg agaccttcat caccatggcg atcatctact tcgtcttcat gaccgcgggc
     6181 gccttcggct accgcattcc ggcgccgggc tggaagcccg agggctggac gccgcccgcg
     6241 ccgagcgcga agtcgatgat gaccgcgcgc cacgtgcacg tgaggcaggc gccgaagacg
     6301 ccgcagttct ggctgatctg gtgggtcctg tgcctgaacg tgaccgccgg catcgacatc
     6361 atcggcatgg cctcgccgat gctgcaggag gtgttcgcgg gcaagctgat cggcatcaac
     6421 gtcgccttca acgacctgac cgccgtgcag cgcggccaga tcgcggcgat cgccgcgggc
     6481 ttcaccgggc tgctcagcct gttcaacatc ggcggccggt tcgtgtgggc gtcgctgtcg
     6541 gatttcatcg ggcgcaagac gacgtattac acgttcttcg tgctcggcat cgcgctctat
     6601 gcctcggcgc cctggtccgg gcacctggga catatcagcc tgttcgtcgg cgccttctgc
     6661 atcatcctgt cgatgtacgg cggcgggttc tccacggtgc cggcgtatct cgccgacatg
     6721 ttcggcacac agatggtggg cgcgatccac gggctgatcc tgacggcatg gtcggccgcg
     6781 ggcatcttcg gccccgtgat cgtgaactac atgcgcgagt tccagctggc gcacggcgtg
     6841 ccgcgcgcgc aggtgtacga cacgacggcc tacatcctgt gcgggctgct cgcgatcggc
     6901 ttcatctgca acctgctgat caagcccgtg gcggacaagc acttcatgac cgacgccgag
     6961 ctcgacgccg agcgcaagct cgcgcacgag gcgtcgcgtg cggcgaccgc gtcctcgcgg
     7021 ggcccggccg acagcgccaa gacgccgacg gcgatcgtcg tgggcgcctg gctggtggtc
     7081 ggcatcccga tcatctgggg cgtctgggtc acgctcgaga aagcctgggt gctgttccgc
     7141 taggtttttc ctgaaacgat ccgcgtccgg tgcgccaaac gcaccggacg cggggtccgg
     7201 aaaattgatg gcagtcaagc ctctaccgga ggccccggcg gtacaatcgt gtcgatgaac
     7261 gatcgtgccg ctccccccga ttccccgacc cgcaagcccg gcaaaggccc ccgcgtgcgt
     7321 gccacgccca agggccggat agtcgatccg aaggcccgcg aggaagtgct cacgttgctc
     7381 ggcgcgaatc cgcgccggcg cgacctgctg atcgagtacc tgcaccgcat ccaggaccgc
     7441 tacggccacc tcgcggcgcc ccacctcgtc gcgctcgccg ccgagctgaa gatggcgatg
     7501 accgaggtgt acgaggtcgc gaccttctat catcatttcg acgtcgtcaa ggaaggcgag
     7561 acgg
//
LOCUS       DATDMW010000035         5382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2124565, whole genome shotgun sequence.
ACCESSION   DATDMW010000035 DATDMW010000000
VERSION     DATDMW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124565"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..540
                     /gene="rpsQ"
                     /locus_tag="VI319_01195"
     CDS             289..540
                     /gene="rpsQ"
                     /locus_tag="VI319_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY6862487.1"
                     /translation="MRGRSQERRGVVVSDAMDKTIVVKVDTVKAHPKYKKVVRRSTKF
                     HAHDEQNSAKVGDLVRIVETRPLSKTKNWRLAEVLEEAK"
     gene            545..913
                     /gene="rplN"
                     /locus_tag="VI319_01200"
     CDS             545..913
                     /gene="rplN"
                     /locus_tag="VI319_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY6862488.1"
                     /translation="MIQQESRLRVADNTGAREILCIRVKGGSKRRYARVGDVITATVK
                     SASPQGAVKKGEVVEAVVVRTRKPFGRNDGTSIAFDENAAVLIDTARNPRGTRIFGPV
                     ARELREKNFMKIVSLAPEVL"
     gene            913..1284
                     /gene="rplX"
                     /locus_tag="VI319_01205"
     CDS             913..1284
                     /gene="rplX"
                     /locus_tag="VI319_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY6862489.1"
                     /translation="MAHKMRVRKGDLVHMLSGKDRGKQGRVLEARPREKRVIVENLNV
                     VKRHTRPRPMRDANRMGGAQMIPGGVIEKSAPVPVANVMLVCPTCNRPTRVGMRMKEV
                     KGREVPVRVCKRADCGEEIDK"
     gene            1308..1862
                     /gene="rplE"
                     /locus_tag="VI319_01210"
     CDS             1308..1862
                     /gene="rplE"
                     /locus_tag="VI319_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY6862490.1"
                     /translation="MKTTYEQEIRPRLKDELGLSSTMQVPRLRKITLNMGVGEAKTDA
                     KVLDSAIEELTTIAGQRAQVRRARKSIAGFKVREDMPVGARVTLRGARMWEFLDRLVS
                     IALPRIRDFRGLNPRSMDGRGNFSLGIREQIIFPEIDYDQIAGIRGLDVAITTTAAND
                     EQGLALLRALGMPFQRETEEGENQ"
     gene            1862..2047
                     /locus_tag="VI319_01215"
     CDS             1862..2047
                     /locus_tag="VI319_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY6862491.1"
                     /translation="MARKALIEKSRKPQRFKTRAYSRCNRCGRPRAVYKKFGLCRICL
                     RDLAHQGVIPGMTKSSW"
     gene            2050..2454
                     /gene="rpsH"
                     /locus_tag="VI319_01220"
     CDS             2050..2454
                     /gene="rpsH"
                     /locus_tag="VI319_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY6862492.1"
                     /translation="MQTDPIADMLTRIRNANRALHERAEMPSSRLKSEIARILKEEGY
                     IQDYRIEHPEDDTPYDTLVIELKYGRRRERVITDLKRVSKPGRRVYAGKDRMPRVLGG
                     MGTAIMSTSNGLITSRAAEEQGVGGEVVCFVW"
     gene            2458..2991
                     /gene="rplF"
                     /locus_tag="VI319_01225"
     CDS             2458..2991
                     /gene="rplF"
                     /locus_tag="VI319_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY6862493.1"
                     /translation="MSRIGRQPIELPAGVSVSVSPGRVMVNGPLGELSQQVPSRMQVE
                     QQNSTIVVSRPTERGEDRALHGLTRTLIANMVEGVTSGFQKRLEIQGVSYRAALRGTD
                     LELNVGYSHSVVLKAPAGITFEVPTPTEVIVKGIDKQAVGQIAAEVRKVRPPEPYKGK
                     GIRYEGEYVRRKVGKRA"
     gene            2988..3341
                     /gene="rplR"
                     /locus_tag="VI319_01230"
     CDS             2988..3341
                     /gene="rplR"
                     /locus_tag="VI319_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY6862494.1"
                     /translation="MSTLTVREARQRRHRRVRGKVFGTAERPRLVVFRSNRGIEAQVV
                     DDLEGKTLAAASWLNLKSFKGSKTEQAAEVGKLLAQNAKGAGVETVIFDRGGYLYHGR
                     VKALADAAREGGLKF"
     gene            3342..3836
                     /gene="rpsE"
                     /locus_tag="VI319_01235"
                     /pseudo
     CDS             3342..3836
                     /gene="rpsE"
                     /locus_tag="VI319_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            3913..4095
                     /gene="rpmD"
                     /locus_tag="VI319_01240"
     CDS             3913..4095
                     /gene="rpmD"
                     /locus_tag="VI319_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY6862495.1"
                     /translation="MAKLKITQTRSAIGQSKRHRGTLRALGLGKIGRSAEHAESPQLD
                     GMLRKVRHLVRVDSDA"
     gene            4088..4714
                     /gene="rplO"
                     /locus_tag="VI319_01245"
     CDS             4088..4714
                     /gene="rplO"
                     /locus_tag="VI319_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY6862496.1"
                     /translation="MPDDLNLSNLQPAQPRKSRKRVGRGLGSGKGRYSGRGLKGQKSR
                     SGSHKMRAGFEGGQNPLYMRLGKQRGPYSRDAMPMGPHRTSTQPVNVAALEERFDAGA
                     EVTLESLVEKGLIRNTRIDVKLLGQGDLKKKLSITVHAASASAREKVEAAGGSLTLLR
                     EPKERRPKRRTQRPAAPGTEETGPEAEEPQAEEPQAEETGPEAEEEES"
     gene            4715..>5382
                     /locus_tag="VI319_01250"
     CDS             4715..>5382
                     /locus_tag="VI319_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY6862497.1"
                     /translation="MFSWLGNAWRVPELRRRLLFTALILALYRLGSWIPAPGVDSQQI
                     KNYFNNQGGTILGLLNVFSGGALSQFSLFALGIMPYVTASIILQLLTVVIPKLEELQK
                     EGESGYAKINQYTRYLTVALAAAQATGYAYLFQRQGALTNNSGRLVLIIITLTAGTTL
                     LMYMGELITKRGIGNGISLLIFASILVSAPAGVTAWYNGGAMEKLFFPLIAIGIIVAV
                     VFIQ"
BASE COUNT          975 a         1825 c         1896 g          686 t
ORIGIN      
        1 gaggagcctg cggcagagga gcctgcggcc gacgagcctg tcgcggagga gcccgccgcc
       61 gaggagccgg tggccgagga gcctcctgcc gacgagccgg cggaagccga agcagagccc
      121 accgccgcgg cagaggagcc ggccgaagcc gaagcccccg ccgctcagcc gtcggcgaag
      181 ccgaagcgca agcggctgcc gcgctcgcag cgccacaagc acgtcaaacc gaagcgcgag
      241 cggccggccc agcgccgccc gatcgtccgg ctggagaagc cggagcacgt gcgcggccgc
      301 agccaggagc gccgcggcgt cgtcgtctcc gacgcgatgg acaagacgat cgtcgtcaag
      361 gtcgacacgg tgaaggcgca tccgaagtac aagaaggtcg tccgccgctc gacgaagttc
      421 cacgcccacg acgagcagaa ctcggcgaag gtcggcgacc ttgtccgcat cgtcgagacc
      481 cggccgctga gcaagacgaa gaactggcgc ctcgccgagg tgctcgagga ggcgaagtag
      541 cgggatgatc cagcaggagt cacggctgcg cgtcgccgac aacacgggcg cgcgcgagat
      601 cctctgcatc cgcgtcaagg gcggctcgaa gcgccgctac gcgcgcgtgg gcgacgtgat
      661 caccgcgacg gtcaagtccg cgagcccgca gggcgccgtg aagaagggcg aggtggtcga
      721 ggccgtcgtc gtccggacgc gcaagccctt cggccgcaac gacggcacct cgatcgcctt
      781 cgacgagaac gcggcggtgc tgatcgacac ggcgcgcaac cctcgcggca cccgcatctt
      841 cgggccggtg gcccgcgagc tgcgcgagaa gaacttcatg aagatcgtca gcctggcccc
      901 ggaggtcctc tgatggccca caagatgcga gtacggaaag gcgacctcgt ccacatgctc
      961 tcgggcaagg accgtggcaa gcagggccgc gtgctcgagg cgcgcccgcg cgagaagcgc
     1021 gtgatcgtcg agaacctgaa cgtcgtcaag cggcacacgc gcccgcgccc gatgcgcgac
     1081 gcgaaccgga tgggcggcgc gcagatgatc cccggcggcg tgatcgagaa gtccgcgccg
     1141 gtgccggtcg cgaacgtgat gctggtctgc ccgacctgca accgcccgac ccgcgtcggg
     1201 atgcggatga aggaagtcaa gggccgcgag gtcccggtgc gcgtctgcaa gcgcgctgac
     1261 tgcggcgagg agatcgacaa gtgagcgcca acggctacga gccccgcctg aagacgacct
     1321 acgagcagga gatccggccg cgcctgaagg acgagctcgg cctgagctcg accatgcagg
     1381 tgccgcggct ccggaagatc acgctcaaca tgggcgtcgg cgaggccaag acggacgcga
     1441 aggtgctcga cagcgcgatc gaggagctga cgacgatcgc cggccagcgc gcgcaggtgc
     1501 gccgtgcccg caagtcgatc gccggcttca aggtgcgcga ggacatgccg gtcggcgcgc
     1561 gggtcacgct gcgcggcgcc cggatgtggg agttcctcga ccggctggtg tcgatcgcgc
     1621 tcccgcgcat ccgcgacttc cgcggcctca acccgcgctc gatggacggc cgcggcaact
     1681 tctcgctcgg gatccgcgaa cagatcatct tccccgagat cgactacgac cagatcgccg
     1741 gcatccgcgg gctggatgtc gcgatcacga ccacggctgc gaacgacgag cagggcctcg
     1801 ccctcctgcg ggccctcggc atgccgttcc agcgcgagac cgaagaagga gagaaccagt
     1861 agtggcacgc aaggcgttga tcgagaagtc gcgcaagcca cagcggttca agacgcgcgc
     1921 gtactcgcgc tgcaaccgct gcggccgccc ccgcgcggtc tacaagaagt tcgggctctg
     1981 ccgcatctgc ctgcgcgacc tcgcgcacca gggcgtcatc cccggaatga ccaagtcgag
     2041 ctggtagcca tgcagaccga tccgatcgca gacatgctca cgaggatccg caacgcgaac
     2101 cgcgcgctgc acgagcgcgc cgagatgccc agctcgcgcc tcaagagcga gatcgcgcgc
     2161 atcctcaagg aggagggcta catccaggac taccgcatcg agcaccccga ggacgacacg
     2221 ccgtacgaca cgctcgtgat cgagctgaag tacggccgtc gccgcgagcg cgtgatcacc
     2281 gacctgaagc gcgtctcgaa gcccgggcgc cgcgtctatg cgggcaagga tcgtatgccg
     2341 cgcgtcctcg gcggaatggg caccgcgatc atgtccacgt ccaacggcct gatcacgagc
     2401 cgcgccgccg aggagcaggg cgtcggcggc gaggtcgtct gcttcgtctg gtgagccatg
     2461 agtcgaatcg gtagacagcc catcgagctt cccgccggcg tgtccgtctc ggtctcgccc
     2521 ggccgcgtga tggtgaacgg cccgctcggc gagttgagcc agcaggtccc gtcgcgcatg
     2581 caggtcgagc agcagaacag cacgatcgtc gtctcgcgcc cgaccgaacg cggagaggac
     2641 cgcgcgctcc acgggctcac gcgcacgctg atcgcgaaca tggtcgaggg cgtgacgagc
     2701 ggcttccaga agcgcctcga gatccaggga gtcagctacc gcgccgcgct ccgcgggacc
     2761 gacctcgagc tcaacgtcgg ctactcgcac tcggtggtgc tgaaggcgcc cgctggcatc
     2821 accttcgagg tgccgacgcc gaccgaggtg atcgtcaagg ggatcgacaa gcaggccgtc
     2881 ggccagatcg ccgccgaggt gcgcaaggtg cgcccgcccg agccctacaa gggcaaggga
     2941 atccgctacg agggcgagta cgtccgccgg aaggtcggga agcgcgcatg agcacgctca
     3001 ccgttcgcga agccaggcaa cggcggcatc gtcgcgtgcg cggcaaggtc ttcggcaccg
     3061 ccgagcggcc gcgcctcgtc gtgttccgct cgaaccgcgg catcgaggcg caggtcgtcg
     3121 acgacctcga gggcaagacc ctcgcggctg cgagctggct caacctgaag tcgttcaagg
     3181 gctcgaagac cgagcaggcg gccgaggtcg gcaagctgct cgcgcagaac gcgaaagggg
     3241 ccggcgtcga gacggtgatc ttcgaccgcg gcggctacct ctaccacggc cgcgtgaagg
     3301 cgctggccga tgcggcacgc gaaggaggac tcaagttcta gatggcaatt gacgtggagc
     3361 agtccagaga gctcaaggag cgcgtggtcg agatcaaccg cgtcgccaag gtggtcaagg
     3421 gcggccggcg gttctcgttc accgcgctcg tcgtcatcgg cgacgaggtc gatcgcgtcg
     3481 gcctgggcta cggcaaggcg cgcgaggtgc cgctggcgat ctcgaaggcg gtcgacgacg
     3541 cgaagaagaa cctgttcacc gtgccgcggc acgggacgac gatcacgcac gagatcctcg
     3601 gccaatttgg cgcggcgcgg gtgctgctgc ggccggcgag cgaggggacg ggcgtgatcg
     3661 ccggcggcgg cgtccgcgcc gtgctcgagc tggccgggat ccgcgacatc ctcgcgaaga
     3721 gcctcggcac gacgaacccg atcaacatgc tgaaggcgac cgtgcagggg ttgcagagcc
     3781 tgcgtcgccc cgcagaggtt gccgccacgc gcgggctgac gatcagccag gtgctgcccg
     3841 ttaccgcgaa gaaggccgag gagcaagagg aggctccggc cgcagcggcg gccgacgagc
     3901 ccgagacgag cgatggcgaa gctgaagatc acgcagacgc gtagcgcgat cggccagagc
     3961 aagcggcatc gcggcacgct gcgagcgctc ggcctgggca agatcggccg cagcgctgag
     4021 cacgctgaga gcccgcagct cgacggtatg ctgaggaagg tccgtcacct cgtgagggtg
     4081 gactccgatg cctgacgacc tgaacctctc gaacctccag cccgcccagc cgcgcaagag
     4141 ccgcaagcgc gtgggccgcg gcctgggctc gggcaagggt cgctactccg gccgcggcct
     4201 caagggccag aagtcgcgct cgggctcgca caagatgcgg gccgggttcg agggcggcca
     4261 gaacccgctc tacatgcggc tcggcaagca gcgcggcccg tactcgaggg acgcgatgcc
     4321 gatgggcccg caccggacct ccacgcagcc ggtgaacgtc gcggcgctcg aggagcgctt
     4381 cgacgccggc gccgaggtca cgctcgagtc gctcgtcgag aagggcctga tccgcaacac
     4441 gcggatcgac gtcaagctgc tcggccaggg cgacttgaag aagaagctca gcatcaccgt
     4501 gcacgccgcc tccgcgagcg cgcgcgagaa ggttgaggcc gccggcggct cgttgacgct
     4561 gctccgcgag ccgaaggagc gcaggccgaa gcgccgcacc cagcgtccgg cggcgcccgg
     4621 gactgaggaa accgggccgg aggcagagga gccgcaggcc gaagagccgc aggcagaaga
     4681 gaccggaccg gaggcagagg aggaggagag ctaggtgttt tcctggctcg gcaacgcctg
     4741 gcgcgtcccc gagctgcgcc ggcggctcct gttcacggcg ctcatcctcg cgctctaccg
     4801 tctgggctcc tggattcccg ccccgggcgt ggactcgcag cagatcaaga actacttcaa
     4861 caaccagggc ggcacgatcc tcggcctgct gaacgtcttc tcgggaggcg cgctctcgca
     4921 gttctccctg ttcgcgctcg ggatcatgcc ctacgtcacc gcctcgatca tcctgcagct
     4981 cctgaccgtc gtgatcccga agctggagga gctgcagaag gagggcgagt ccggctacgc
     5041 caagatcaac cagtacacgc gctacctgac cgtggctctg gcggccgcgc aggcgaccgg
     5101 gtacgcatac ctcttccagc ggcagggcgc gctgacgaac aactcgggcc gcctggtgct
     5161 gatcatcatc acgctcaccg ccggcacgac gctgctcatg tacatgggcg agctgatcac
     5221 caagcgcggc atcggcaacg gcatctcgct gctgatcttc gcgtcgatcc tcgtctcggc
     5281 gcccgcgggc gtgactgcct ggtacaacgg cggcgcgatg gagaagctgt tcttcccgct
     5341 gatcgcgatc gggatcatcg tcgcggtcgt cttcatccag ga
//
LOCUS       DATDMW010000036         3380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2000057, whole genome shotgun sequence.
ACCESSION   DATDMW010000036 DATDMW010000000
VERSION     DATDMW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000057"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1420
                     /locus_tag="VI319_01255"
     CDS             <1..1420
                     /locus_tag="VI319_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6862498.1"
                     /translation="LGARLAAVPEVTDYQAYAGMSSPIGFNGLVRQYYLRTASEQGDL
                     QVNLADKKQRSRSSHEIAQAVRPALEEVGRRWSARVKVVEVPPGPPVLSPVVAEVYGP
                     DEPGRIRVAKRVREALASTPGIVGIDDSVDDAAPRLLLRIDQGRAALLGVTRRDVVET
                     MRVGLSGADVTPVHDGASKYEVPVRLALPAERQGELDEFLKLTVRTPAGTLVPLSEVV
                     RAARTDRDRPLYHKDLLPVVYVVADQAGAIDSPLYGMFAARAKIAGVPLPEGGTLAEY
                     FVRQPSDPYRGYAVKWDGEWQVTYETFRDMGLAYAVGLVLIYLLVVAQFRSYLTPIVI
                     MAPIPLTVIGIMPGHALLGANFTATSMIGMIALAGIIVRNSILLVDFINLKTEEGVAF
                     AEAVVQSAVTRAKPIALTGLAAMMGAAFILDDPIFNGLAISLVFGIFVSTMLTLVVIP
                     VFYYAAYRGRFEAAPVDGGTRT"
     gene            1417..2049
                     /locus_tag="VI319_01260"
     CDS             1417..2049
                     /locus_tag="VI319_01260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6862499.1"
                     /translation="MSDAPAGAELERWNKRFSAEDYVFGTEPNAFLASQKDRLAPGMS
                     ALCVADGEGRNSVWLARQGLRVAAFDFSPVGLQKARALARKAGVAVDYRLCEVGGWDW
                     DAARYDVIAAIFVQFTPPPARARMFAGMARALNPGGLLILEGYRPEQLRYATGGPKQV
                     ENLYTEALLRESFAGLEILHLASYDAEVDEGGGHRGMSALIDLVARRPAR"
     gene            complement(2056..3015)
                     /locus_tag="VI319_01265"
     CDS             complement(2056..3015)
                     /locus_tag="VI319_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862500.1"
                     /translation="MIRMLAALAALCLAVPGAVAQEYPAREIHAVCNYAAGSGADLIV
                     RFYSDQLSHLAGKPVIVENRPGAQGMVANDYVAKSKPDGYTLLITPVSSTVNIAPSIF
                     RKLTYDPKKDLVAVTTIATVSFTLAVNAKSPVRSVGELVARLKSLPKQGFYGATSNAG
                     NITGELFKSMAGLQTTFVPYKANPNALADLLSGQLDFIAFDSTWTLQQQNNGFVRILA
                     VTSAKRSGALPEVPTMQEAGFDGYDVTPWWGVLVPAGTPRPVIDRLAGWFNEITGSDE
                     GRAFLARSAFDPFPGTPESAEKLLQSDFERWARYARMAKIQPQ"
     gene            3121..>3380
                     /locus_tag="VI319_01270"
     CDS             3121..>3380
                     /locus_tag="VI319_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862501.1"
                     /translation="MRTLLLASALALAAGVALAQGPGPGGGPGKGPGGGWRFGPDNTR
                     GWSMMTPEERNEHRQKMLGAGTVAECQAVLEEHRKLMEERAK"
BASE COUNT          498 a         1196 c         1172 g          514 t
ORIGIN      
        1 cctcggcgcg cgcctcgccg cggtccccga ggtgaccgac taccaggcat acgccggcat
       61 gagctcgccg atcgggttca acggcctcgt gcgccagtac tacctgcgca cggcgtccga
      121 gcagggcgac ctgcaggtca acctggccga caagaagcag cgctcgcgct cgagccacga
      181 gatcgcgcag gccgtgcggc ccgcgctcga ggaggtcgga cgccgctgga gcgcgcgcgt
      241 gaaggtcgtc gaggtcccgc ccggaccgcc ggtgctctcg ccggtcgtcg ccgaggtcta
      301 cgggcccgac gagccgggcc gcatccgcgt agccaagcgc gtgcgcgagg cgctcgcctc
      361 gacgccaggc atcgtcggca tcgacgacag tgtcgacgac gccgcgccgc gcctgctgct
      421 gcggatcgac cagggacgcg cggcgctgct cggcgtgact cggcgcgacg tcgtcgaaac
      481 catgcgcgtc ggcctgtccg gggccgacgt gacaccggtt cacgacggcg cgtcgaagta
      541 cgaggtcccg gtgcggctcg cgctgccggc cgagcgccag ggcgagctcg acgagttcct
      601 caagctcacg gtgcgcacgc ccgcggggac gctggtgccg ctgtccgagg tggtgcgcgc
      661 cgctcgcacc gaccgcgacc ggccgctcta ccacaaggac ctcctgccag tcgtctacgt
      721 cgtcgcggac caggccggcg cgatcgacag ccccctctac ggcatgttcg ccgcgcgcgc
      781 gaagatcgcc ggcgtcccgc tccccgaagg cgggacgctc gccgagtatt tcgtgcgcca
      841 gccatccgac ccgtaccgcg ggtacgcggt gaagtgggac ggcgagtggc aggtgaccta
      901 cgagaccttc cgcgacatgg gcctcgccta cgccgtcggc ctagtgctca tctacctgct
      961 ggtcgtcgcg cagttccggt cgtatctgac gcctatcgtg atcatggcgc cgatcccgct
     1021 cacggtgatc ggcatcatgc ccggccacgc cctgctcggc gcgaacttca ccgccacctc
     1081 gatgatcggc atgatcgcgc tcgccggcat catcgtgcgc aactccatcc tgctggtcga
     1141 cttcatcaac ctgaagaccg aggaaggcgt cgcgttcgcc gaggccgtcg tgcagtcggc
     1201 cgtgacgcgc gcgaagccga tcgcgctcac cggcctcgcc gcgatgatgg gcgcggcgtt
     1261 catcctcgac gatcccattt tcaacggcct cgcgatctcg ctcgtgttcg gcatcttcgt
     1321 ctcgacgatg ctcacgctgg tcgtgatccc cgtgttctac tatgctgcgt accgcggccg
     1381 cttcgaggcc gcgccggtcg acggaggaac acggacgtga gcgatgcacc cgcgggcgcc
     1441 gagctcgagc gctggaacaa acgcttctcg gccgaggatt acgtcttcgg caccgagccg
     1501 aacgccttcc tcgcttccca gaaggaccgc ctcgcgcccg gcatgagcgc gctctgcgtc
     1561 gccgacggcg agggccggaa cagcgtctgg ctcgcgcgcc aggggctgcg ggtcgcggca
     1621 ttcgacttct cgcccgtcgg cctgcagaag gcgcgagcgc tcgcgcgcaa ggcgggcgtc
     1681 gccgtcgact accgcctgtg cgaggtcggc ggctgggact gggacgccgc gcgctacgac
     1741 gtgatcgcgg cgattttcgt gcagttcacg ccgccacccg cgcgcgcgcg aatgttcgcc
     1801 gggatggcgc gcgcgctgaa tccgggcggc ctgctgatcc tcgaaggcta ccgtcccgag
     1861 cagctgcgct acgcgacggg aggcccaaag caggtcgaga acctctacac cgaagcgctg
     1921 ctgcgcgagt ccttcgccgg gctggagatc ctgcacctcg cgtcgtacga cgccgaggtc
     1981 gacgaaggcg gcgggcaccg cggcatgtcg gcgctgatcg atctcgtcgc gcggcgcccg
     2041 gcccgctagc gccgcttact gcggctggat cttcgccatc cgcgcgtagc gggcccagcg
     2101 ctcgaagtcg gactgcagca gcttctcggc cgattccggc gtccccggga acgggtcgaa
     2161 cgccgagcgc gcgaggaatg cccggccctc gtcggagccg gtgatctcgt tgaaccagcc
     2221 ggcgagcctg tcgatcacgg gacgcggcgt gccggccggg acgaggacgc cccaccaggg
     2281 cgtgacgtcg tagccgtcga acccggcttc ctgcatggtg ggcacctcgg gcagcgcccc
     2341 cgagcgcttc gccgaggtca ccgcgaggat gcgcacgaag ccgttgttct gctgctgcag
     2401 cgtccaggtc gagtcgaacg cgatgaagtc gagctgcccc gagagcaggt cggcgagcgc
     2461 gttcgggttc gccttgtacg ggacgaaggt cgtctgcagg cccgccatcg acttgaacag
     2521 ctcccccgtg atgttgcccg cgttgctggt cgcgccgtag aagccctgct tcggcagcga
     2581 cttcaggcgc gccaccagct cgcctacgct gcgcaccggg ctcttcgcgt tcacggcgag
     2641 ggtgaagctc accgtcgcga tcgtggtcac ggcgacgagg tccttcttcg ggtcgtaggt
     2701 gagcttgcgg aagatcgacg gcgcgatgtt gaccgtcgag ctgaccggcg tgatcagcag
     2761 ggtgtagccg tcgggcttcg acttcgcgac gtagtcgttg gcgaccatcc cctgcgcgcc
     2821 cggccggttc tcgacgatca ccggcttgcc ggcgaggtgc gagagctggt cgctgtagaa
     2881 gcgcacgatg aggtccgcgc ccgagccggc ggcgtagttg cagaccgcgt ggatctcccg
     2941 ggcgggatat tcctgggcca cggccccggg aacggcgagg caaagggccg cgagcgcggc
     3001 gagcatcctg atcatgagcg gtaacctcct cgccccgatt ctaattgatc gacgtcaacg
     3061 ggcgcgcccg cacggcgcct acgatgaacc ggcaacccga caatcatcga aggagacatc
     3121 atgagaaccc tgttgctcgc ttcggcgctc gcgctcgccg ccggcgtcgc cctcgctcaa
     3181 ggtcccggtc ccggaggcgg tcccggcaaa ggccccggcg gcggctggcg gttcggcccg
     3241 gacaacaccc gcggctggtc gatgatgacg cccgaggagc gcaacgagca ccggcagaag
     3301 atgctcggcg ccggcaccgt ggccgagtgc caggccgtgc tcgaggagca ccgcaagctg
     3361 atggaagagc gcgcgaagga
//
LOCUS       DATDMW010000037         3004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1752144, whole genome shotgun sequence.
ACCESSION   DATDMW010000037 DATDMW010000000
VERSION     DATDMW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752144"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VI319_01275"
     CDS             complement(<1..867)
                     /locus_tag="VI319_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6862502.1"
                     /translation="MPHFRADDGVRLHYEETGAGAPLVFVHEYAGDSRSWEAQLRFFG
                     RYYRSIAYNARGYPPSDVPEDPEMYSQARARDDIRSLLDHLGIDRAHVCGLSMGGFAT
                     LHFGMAYAERARSLVVAGCGYGAEPAKRAQFRDESHAAAAQIESEGMAEVAKRYALGP
                     ARVQLQNKRPRAWREFAEQLAGHSARGSALTLRGVQGRRPSLWDLQDALRALRVPTLI
                     VAGDEDEPCLEPALLMKRAIPTAGLVVMPCAGHAVNLEEPEAFNRIVFDFLAAVDAGA
                     WRARDPRSISAGI"
     gene            complement(870..1826)
                     /locus_tag="VI319_01280"
     CDS             complement(870..1826)
                     /locus_tag="VI319_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862503.1"
                     /translation="MLCLRFLAALALAALPALAAAQDYPAKPVHLIVSFPGGSSDVLG
                     RAFAQFARLGQPIVVENMSGANGAIGIGRLAKSPPDGYTFAIGATTNLAIGPHLNSKL
                     PYDPLRDFAPVALLARVTIVLVVSSAVPAKSVDELVALARSRPGKLNYGSIGTGSTTH
                     LLGEMLRRSAGIDIVHVPYKGIQQAVTALLAGDVQFIFFPVFGDARAYVRSGALRPLA
                     VVQSTRSPVAPELPTLPELGYPLEAAAWFALLAPAGTPDAIVQRLADEVRRVLALEPM
                     RRFLDDHGMEAGDLGPEAVKAYIAAEHARYGRMVREAGVRVE"
     gene            1853..2662
                     /locus_tag="VI319_01285"
     CDS             1853..2662
                     /locus_tag="VI319_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6862504.1"
                     /translation="MFAQINHVAIISHQYNTLGKFYEAVFGLKAYGNRPASAITIGDG
                     YVGLNLIPRRDGYVGGIDHFGMLVDDVGPVLERMQRKHRSAALVKRPSTRPYAAYSGH
                     DPDGNVFDLAQKKADTRKEVYAEIAGGGWAQDRYINKFAIRTPNAEACAEFYRDVFEL
                     EPVRGRWGAPAYCLSDGRVTLAILPWAIPMFEGMSIKRPGPDHFGFKVEDLAAFKAHL
                     AEVAGMNPYLAPVPLGGSKESDVRRRFLESSATGKFQIADPDGVWIDITDE"
     gene            complement(2663..>3004)
                     /locus_tag="VI319_01290"
     CDS             complement(2663..>3004)
                     /locus_tag="VI319_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862505.1"
                     /translation="EVFELKVTSANGQFALSDGRVTLRILPWRLADYYGQDPARTGLD
                     HIGFKVESVEAVKRDMAELVGVNPQMVTRPLGCGSEGEARLAVLKQCPVGAFHLTDIE
                     GVYIDVAEGGA"
BASE COUNT          464 a         1054 c         1080 g          406 t
ORIGIN      
        1 gatgccggcg ctgatcgagc gcggatcgcg cgcgcgccag gcgcccgcgt cgacggcggc
       61 gaggaaatcg aagacgatgc ggttgaacgc ctcgggctcc tcgaggttca cggcgtgccc
      121 ggcgcagggc atcacgacca gcccggcggt cgggatcgcg cgcttcatga gcagcgcggg
      181 ctcgaggcag ggctcgtcct cgtccccggc gacgatcagc gtcggcacgc gcagggcgcg
      241 cagggcgtcc tgcagatccc acagcgacgg ccggcggccc tgcacgccgc gcagcgtgag
      301 cgccgagccg cgcgcggagt gtcccgcgag ctgctcggcg aactcgcgcc aggcgcgcgg
      361 gcgcttgttc tgcagctgca cccgcgccgg cccgagcgcg tagcgcttcg ccacctcggc
      421 catgccttcg ctctcgatct gcgcggccgc ggcgtgcgac tcgtcgcgga actgcgcgcg
      481 cttcgcgggc tcggcgccgt agccgcagcc cgcgacgacg agcgagcgcg cgcgctccgc
      541 gtacgccatg ccgaaatgca gcgtggcgaa gccgcccatc gacagcccgc agacgtgcgc
      601 ccggtcgatg ccgaggtgat cgaggagcga gcggatgtcg tcgcgggcgc gcgcctgcga
      661 gtacatttcc gggtcttccg ggacgtccga gggcggatag ccgcgggcgt tgtacgcgat
      721 cgagcggtag tagcgcccga agaaccgcag ctgcgcctcc cagctgcggg agtcgcccgc
      781 gtactcgtgc acgaacacga gcggcgcgcc ggcgccggtc tcctcgtaat ggagccggac
      841 tccgtcgtcg gcgcggaagt gcggcacgat cactcgaccc tgacgccggc ctcgcgcacc
      901 atgcgcccgt agcgcgcgtg ctcggcggcg atgtaggcct tcaccgcttc gggtcccagg
      961 tcgcccgcct ccatcccgtg atcgtcgagg aagcgccgca tcggctcgag cgcgaggacg
     1021 cgccgcacct cgtcggcgag ccgctgcacg atcgcatcgg gcgttcccgc cggcgcgagc
     1081 agcgcgaacc aggcggcggc ctcgagcgga tagccgagct cgggcagcgt cggcagctcg
     1141 ggcgcgacgg gagagcgggt cgactggacc accgcgagcg gccgcagcgc gcccgagcgt
     1201 acatacgctc gcgcgtcgcc gaacaccggg aagaagatga actgcacgtc tcccgcgagc
     1261 agggccgtca cggcctgctg gatgcccttg taggggacgt gaacgatgtc gatgccggcg
     1321 ctccgccgca gcatctcgcc gagcaggtgg gtggtggacc cggtgccgat cgagccgtag
     1381 ttgagcttcc cgggacgcga gcgcgcgagg gcgacgagct cgtcgaccga tttcgccggc
     1441 acggccgagc tcaccaccag cacgatggtc acgcgcgcga gcagcgcgac cggcgcgaaa
     1501 tcccggagcg gatcgtaggg gagcttcgag ttgagatggg ggccgatggc gaggttggtg
     1561 gtggcgccga tcgcgaaggt atagccgtcg ggaggggact tcgccaggcg cccgatcccg
     1621 atcgccccgt tcgcgccgga catgttctcg accacgatcg gctggccgag cctcgcgaac
     1681 tgcgcgaagg cgcggcccag gacgtcgctc gagcccccgg gaaaggagac gatgaggtgg
     1741 accggcttcg ccgggtagtc ctgcgccgcg gcgagggcgg gaagcgcggc cagcgcgagc
     1801 gccgcgagga agcgaaggca aagcatcggg ttgatggtag attactccgg ccatgttcgc
     1861 ccagatcaac cacgtggcca tcatcagcca ccagtacaac acgctcggca agttctacga
     1921 ggccgtgttc gggctgaagg cctacggcaa ccggccggcc tcggcgatca cgatcggcga
     1981 cggctacgtc ggcctgaacc tcattccccg gcgcgacggc tacgtcgggg gcatcgatca
     2041 tttcggcatg ctggtcgacg acgtcgggcc cgtgctcgag cgcatgcagc gcaagcaccg
     2101 gagcgcggcg ctcgtgaagc ggccctcgac gcgtccctac gccgcctaca gcggccacga
     2161 tcccgacggc aacgtcttcg acctcgcgca gaagaaggcc gacacgcgca aggaggtcta
     2221 cgccgagatc gcgggcggcg gctgggcgca ggaccgctac atcaacaagt tcgcgatccg
     2281 cacgccgaac gcggaagcct gcgcggagtt ctaccgcgac gtgttcgagc tcgagccggt
     2341 gcgcggccgg tggggggcgc cggcttattg cctcagcgac gggcgcgtga cgctcgcgat
     2401 cctgccatgg gcgatcccga tgttcgaggg catgtcgatc aagcgcccgg gaccggatca
     2461 cttcggcttc aaggtcgagg acctcgcggc gttcaaggcg cacctcgccg aggtcgcggg
     2521 gatgaatccc tatctcgcgc cggtgccgct cggcggcagc aaggagtccg acgtgcgccg
     2581 gcgcttcctc gagtcgagcg ccaccgggaa attccagatc gccgatcccg acggcgtctg
     2641 gatcgacatc accgacgaat aatcaggcgc cgccctcggc gacgtcgatg tacacgccct
     2701 cgatgtcggt gaggtggaac gcgcccaccg ggcactgctt cagcaccgcg agccgcgcct
     2761 cgccctcgct gccgcagccc aggggccgcg tcaccatctg cgggttgacg ccgaccagct
     2821 cggccatgtc gcgcttcacc gcctcgacgc tctcgacctt gaacccgatg tggtcgagcc
     2881 cggtgcgcgc cgggtcctgg ccgtagtagt cggcgaggcg ccagggcagg atgcgcagcg
     2941 tcacgcgccc gtcggagagc gcgaactggc cgttcgcgct cgtcaccttc agctcgaaca
     3001 cctc
//
LOCUS       DATDMW010000038        14727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421 k141_9802,
            whole genome shotgun sequence.
ACCESSION   DATDMW010000038 DATDMW010000000
VERSION     DATDMW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14727
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9802"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1113
                     /locus_tag="VI319_01295"
     CDS             109..1113
                     /locus_tag="VI319_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6862506.1"
                     /translation="MNAPLPKFVQLEPVGQCNLRCRMCPIQFRGDGSPGGPPAFMSYE
                     LFCRLVDGFPALTELHLQGLGEPLMHPRFFDMVRYASSRGIEVSTNTNMTVLSERRAE
                     ECVASGLARVHVSLDGASAETYEFIRVRARFERVLRNLRRLAEAKARLRGARPEIRLV
                     AVAMRRNLDELPALVRLAHELGIAHVSVQHLCHDFGEASLPEQYRPMRAFVEDETLLH
                     EDPERVRRAFDGARAAAAELGVALRLPNVRPRGHASAVPGRSRCDWPWRGAYVSWSGE
                     AMPCCMVATPDRINFGSMAKQGVAEVWSNEAYSEFRRRLDSDDPPEICRSCAVYHGTF
                     "
     gene            1117..2103
                     /locus_tag="VI319_01300"
     CDS             1117..2103
                     /locus_tag="VI319_01300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6862507.1"
                     /translation="MRRLKILTWHTHGAYLYYLTQAPHEFYVLSKPDRPPGYGGRCGH
                     MPWGANVHDLPVEEARHAQLDCIVFQDDAHWLEDQHQWLTPEQRRLPRIYLEHDPPRE
                     SPTDAHHPVDDPGVLLVHVTPFNALMWDSGRTPTRVIEHGVIAPPRALYTGDYARGIV
                     IVNNLARRGRRLGSDIFKAARAQVPLDLIGIGAEELGGIGEVPHRDIPAFAARYRFLF
                     NPIRWTSMGLAVIEAMMAGVPVVGLATTEMARAIENGVSGWVDTSPARLVERMRVLLD
                     DRNLARALGAGARRRAIERFGIERFVRDWNAAFAQVTGDRAPVAEGSVAHAA"
     gene            2100..2642
                     /locus_tag="VI319_01305"
     CDS             2100..2642
                     /locus_tag="VI319_01305"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6862508.1"
                     /translation="MSTPAVKLNLGCGPNVAPGWLNLDVEPVAGAVQGDLRAGLPLAD
                     SSVDCIAAIHLLQDLPWPEVRSALGEMRRVLKPGGVLRLAVPDLDRALRAYLAGDRAY
                     WYVPDEHAKSGGAKLVTQIVWYGSVRTPCNWDFVREWLLAARYREVRRCAFGVTHAPF
                     PELARLDNRERESLFVEAVR"
     gene            2639..3325
                     /locus_tag="VI319_01310"
     CDS             2639..3325
                     /locus_tag="VI319_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY6862509.1"
                     /translation="MSGALVVSPHLDDAVLACGAWIAAHAGVRVLTVFAGAPRTSAPP
                     TEWDAACGFADAAEAIAARRAEDRAALALLGAEPEWLDWCDDQYGVPAPEGALVADVS
                     ADLRRALERHDPHAVLVPLGLFHRDHERTHDAGLALLEEHRTRTWYAYEDALYRAIPG
                     LVQRRLRRLAERRVTATPEPTPERTRGASGRKRRALACYASQLRGLATPGRPGHADAL
                     AAERLWRLTL"
     gene            3307..4194
                     /locus_tag="VI319_01315"
     CDS             3307..4194
                     /locus_tag="VI319_01315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6862510.1"
                     /translation="MATDAVGRTAGACVSVVILTHNRAPELAGTLARTLALPERPAVI
                     VVDNGSRDGTADLVAARFPQARLVRLSDNYGAAARNVGARAARTPYVAFCDDDTCWQS
                     GSLALAERLLDAHPRVAVLSARVLVGAEGREDPACAAMAASPLPSAGLPGRSVVGFLA
                     GACVFRRDAFLEAGGYEPRFFIGGEEALLAYDLLARGWRIVYSERLLVRHHPSSYRDS
                     RRRRRLLARNALWVAWLRRPLPAALARTARVLLSGIPDRAAIAGCIDALRGLPWVLRN
                     RRVVPPHVEALRRLADAGE"
     gene            complement(4247..4900)
                     /locus_tag="VI319_01320"
     CDS             complement(4247..4900)
                     /locus_tag="VI319_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY6862511.1"
                     /translation="MTRTRIASTLAITGLAAATWCGAGYAQQTQSPSTDAQNQAQSEQ
                     RPTPQNLARDNRGGRDDASDKLARRDRKFMEKVAQDNAAEIEAGKLASSKGSNDQVKQ
                     FGERMVQDHGQAADELKQLAQSKGVDLADTADRKHEREAKSLDKKSGADFDRAYMQQM
                     VKDHRADLKELQKEAKNAKDPDVKAFAERTAQVVQEHLNQAQQIAADVGAKGGSKKG"
     gene            5143..7338
                     /locus_tag="VI319_01325"
     CDS             5143..7338
                     /locus_tag="VI319_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY6862512.1"
                     /translation="MPEAQATDTLFEIGENCCAVKRSNRVALLVDAESYFEAFMRAAE
                     RAERSIVVLAWDFNSRTPLRIGPDGERLLLGDFLNGLARRRRRLHVRVLDWDYPMVFG
                     VDRELPPLYGLSWTPHRRVHLRYDGTHPLAGSHHQKIVVIDDRLAFVGGLDLTARRWD
                     TSGHAPGDPRRIASGKAYPPFHDVMIAVDGEAAASLAEIARKRWRLATHSDIPRVARA
                     GDPWPDSLRPDMTDVDVGVACTAPETSGEKGVRDVERLYLDMIARARRSIYVENQYFT
                     SHTIGEALAARLAEPDGPEIVIVTRLLSHGWLEEMTMQVLRTRLIQALKQVDRYQHFH
                     VYYPDIDGLAEGTCIDVHSKLMVVDDEWLRVGSANLSNRSMGLDTECDVVVEAGARPE
                     LRRAVSAFRDRLLAEHTGAEAADVAREIERSGGMAAAVAKLSTGPRRLRPLDDLKDWP
                     EAVVEAIAVADPERPVSLDQLADEFAQDEVVRERRHVGRNAAIVAFVLLALGLAWRYT
                     PLAEVATPEAVLDWFDRFSGHWWAPLALLAAYTPASLVMFPRPLITLATVIAYGPWTA
                     FAFSIAGNVIAACVVYAAGRKFGRDTVRRIAGERLNRLCSLLQRRGLLAVVAVRLVPV
                     APFVVENVVGGAVRIRFWQFAVGTAIGHVPGTLATTVFGSQIEAALREPGNINWWLVG
                     AATVLLGAAILAMRRWLGRLESREKAREPVRRRAAERALLARQHAELGS"
     gene            7404..8534
                     /locus_tag="VI319_01330"
     CDS             7404..8534
                     /locus_tag="VI319_01330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6862513.1"
                     /translation="MHLVDTTMFFAARAGGVQRYLSAKRAWLRRQGAVRHTIVAPGAA
                     DGEAQPGITTLRTHPRPRPYGFPFPLRLRAWRRALVRLAPDLIEAGDPFTPAWAALRA
                     GQQLGVPAIGFYHSDLVRLVGTRLGPAGQSAAARYVKQLYREFDLVLAPSRYVAARLR
                     ELGLERVVHQPLGVDASVFHPARRDPGLRRALGLPERTRLLVFAGRFAHEKNLPLLLA
                     AFRRLGPRYHLLLVGGGMELGSQRNVTVLDFQTREAELARLVASCDALVHAGEQETFG
                     LVVLEAMACGLPVVGVAAGGVAELVGERHGVLVRPGSATALAEGIEAAYSADVAVLGA
                     RAREAVERRYSWDCVMQSLLGIYHQRLLISSADGVGEAYALR"
     gene            8521..9309
                     /locus_tag="VI319_01335"
     CDS             8521..9309
                     /locus_tag="VI319_01335"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6862514.1"
                     /translation="MRSAERATVCISVHDVAPATWPRCARLLAMLDALGAGPVTLLVV
                     PDYHHGGRIDLYPWFLRAIEERRARGDEIALHGYYHEDEAAPPRGPLGWIERRVLTQS
                     EGEFAALGADEALVRLQRGAALMESLGWTVRGFVAPAWLPGRGCRVALSRMTSGLAGF
                     AYTTTRSGVYRLPDWRYTFSPSLVCTVRSAWRRSMSQSLNAASFALASRGPLLRLSLH
                     PVDAEHADVMARWRDWIARALEHHAPATKLQWIAAEGFVASAAS"
     gene            9367..9585
                     /locus_tag="VI319_01340"
     CDS             9367..9585
                     /locus_tag="VI319_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862515.1"
                     /translation="MDNEKSPSDDSQAGSTERKPARRAQPGAAGLGRGGADGRRSSDT
                     LKRNQERLGVGPDHRTDAMKKGKRGTFP"
     gene            complement(9791..10279)
                     /locus_tag="VI319_01345"
     CDS             complement(9791..10279)
                     /locus_tag="VI319_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862516.1"
                     /translation="MKRGLDTPGLFVDRDAAHEALRLAAPLLERAAGDAGVGQSGVVY
                     VVVMDPSRPACSCGRSCEFEQAILAEQGFGKPRAEWDADYAGYARAKARLAWRTGRDS
                     HAVRALSPHLLRRDDTPLWGSVCVDGIAVGVSGAEPEFDEALSGAVAMCLRAVVKRRA
                     RS"
     gene            complement(10323..13016)
                     /locus_tag="VI319_01350"
     CDS             complement(10323..13016)
                     /locus_tag="VI319_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY6862517.1"
                     /translation="MEEPANRATGLASLWSCAWRFRWRVLAAIVLLVVAKLSIVSVPL
                     VLKRIVDALSRPENVAVLPVFLLLGYALLRFAGTLFGELRDLVFARVGAQTSSDFTAR
                     AFAHLLALTPRFHGGRETGRLIREVERGTLAIGFLLGVGLFTIVPTLVEIAAVMTIMV
                     LNYSSWFAAIISATFAVYTAFTLVFTERRQVHQRRMNELDSRSNARLVDSLLNYDTVK
                     YYANESLEQSRFRSIMREWVEVGVRNQKALSMLHVGQSAIIAVGVAAIMLLAGHDVVR
                     GTMTVGDLVLVNAYVIQVCLPLNALGFVFRQARDAVVNAEKLFALLKQRPDMEEAPGA
                     PALAVREAEVRFEHVSFGYEPARQVLRDVHFRVPPGATVAVVGGSGSGKSTLARLLLR
                     FYDVASGGVYIDGQDVRSVSLKSLRDAIGIVPQDTILFNDTIGYNIAYGRSGASFAEV
                     VEAAKAAQLHDFILALPEQYETVVGERGVKLSGGEKQRIAIARAVLKNPPILIFDEAT
                     SALDPVAERAIQAELERVAQNRTTLIIAHRMSTIVHADDILVLEHGRVVERGTHHELL
                     ERRGIYARMWAIQQQERELAHAERRLALQAVNLAAVAASAVDVLRPEIDAKGIRLYAM
                     IGSQSERVTGDPALLQQMVWDLCANAIAVTPAGGRVEIEVRRNGPTVRLKVVDTGPQP
                     KRAEGALRLDVARLHEAVERHGGRFAVERLPAGGGTGYVVDLPVSAVAGTAELPPPPD
                     GTPRARGGATIPKLDGASIAIVDDDDDARELLATVLEAKGADVRAFARGGEARGWFTR
                     TPHEAWPHLLVCDIGLPDEDGYAVVHGLRALEAERGVPLPERMPAIALTGYARPEDRT
                     QALLAGFQMHLAKPVEPRELIASAGALIGAKPRRAIAGRPT"
     gene            complement(13035..14045)
                     /locus_tag="VI319_01355"
     CDS             complement(13035..14045)
                     /locus_tag="VI319_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY6862518.1"
                     /translation="MRDLLRRRVLVTGGAGFLGSHLCERLLDDGCDVLCVDNFYTGTK
                     DNVAHLAQDPHFELLRHDVTFPLHVEVDEIYNFACPASPIHYQLDPVQTTKTSVHGAI
                     NMLGLAKRAKAKILQASTSEVYGDPEMHPQDEAYWGHVNPVGLRSCYDEGKRCAETLF
                     MDYRRQHGLATKIARIFNTYGPRMHPNDGRVVSNFVVQALQDEPIRVYGDGAQTRAFC
                     YVSDLVDAVVRLMASADDFIGPVNLGNPHEFTIAELADTVIELTGSRSRIVHAKLPAD
                     DPARRCPDIGLARAALGWEPRVPLREGLARTITYFEQRLRTRRAGFDNRRRRESLEEA
                     PR"
     gene            complement(14042..>14727)
                     /locus_tag="VI319_01360"
     CDS             complement(14042..>14727)
                     /locus_tag="VI319_01360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY6862519.1"
                     /translation="EPREHEVLRGLRLLERLGVPGAGIELEFPIDEVDREELARLASA
                     FRFRPGQVVCVHPGAQLASRRWPPERFARVADALAAEGHTVVLTGTAGEAAVTCAVAG
                     AMRSRAIDLTGLTSLGALAALIARARLLVCNDTGVSHVASATRTPSVVVSCGADTARW
                     SPLDPERHRVLAYELVCRPCSYASCPYGHECAQGVAPESVIAETRALLAKFPAGARRA
                     GARAAEVHP"
BASE COUNT         1896 a         5403 c         5338 g         2090 t
ORIGIN      
        1 gcacgtgctc gagatgatga tcacctccgc cgtcatcccg ccgctcgccg tcttctggcg
       61 cgcggtcggc gcctggcgct accgcgtggt cttcgcgtag gcgatcccgt gaacgcaccg
      121 ctgccgaaat tcgtccagct cgagcccgtc ggccaatgca acctgcgctg ccgcatgtgc
      181 ccgatccagt tccgcggcga cggctcgccg ggcgggccgc cggcgttcat gagctacgag
      241 ctgttctgcc ggctggtcga cggcttcccc gcgctcaccg agctgcacct gcagggcctc
      301 ggcgagccgc tcatgcaccc gcgcttcttc gacatggtgc gctacgcgag ctcgcgcggc
      361 atcgaggtct cgaccaacac caacatgacc gtgctctcgg agcgccgcgc cgaggaatgc
      421 gtggcgagcg gcctcgcgcg cgtgcacgtc tcgctcgacg gggcgagcgc cgagacctac
      481 gagttcatcc gagtgcgcgc gcgcttcgag cgcgtgctgc gcaacctgag gcgcctcgcc
      541 gaggcgaagg cgcgcctgcg cggcgcgcgc cccgagatcc gcctggtcgc ggtggcgatg
      601 cgccgcaatc tcgacgagct gcccgcgctc gtccgcctcg cgcacgagct cggcatcgcg
      661 cacgtgtcgg tgcagcacct gtgccacgac ttcggcgagg cgagccttcc cgagcagtac
      721 cggccgatgc gcgccttcgt cgaggacgag acgctcctcc acgaggaccc ggagcgcgtg
      781 cgccgcgcgt tcgacggggc gcgcgcggcc gcggcagagc tcggcgtggc gctgcggctg
      841 ccgaacgtgc gaccccgcgg gcacgcctcc gcggtcccgg ggcgctcgcg ctgcgactgg
      901 ccctggcgcg gcgcgtacgt gagctggtcg ggcgaggcga tgccgtgctg catggtggcg
      961 acgcccgacc gcatcaactt cggcagcatg gccaagcagg gcgtcgccga ggtgtggagc
     1021 aacgaggcgt actcggagtt ccggcgccgg ctcgactccg acgacccgcc cgagatctgc
     1081 cgcagctgcg ccgtctacca cgggaccttc tagcccatgc ggcgactgaa gatactgacc
     1141 tggcacacgc acggcgcgta tctctactac ctgacgcagg cgccgcacga gttctacgtg
     1201 ctgtcgaagc ctgaccgtcc cccgggctac ggcgggcgct gcggacacat gccctggggc
     1261 gcgaacgtgc acgacctgcc ggtcgaggag gcgcgccacg cgcagctcga ctgcatcgtg
     1321 ttccaggacg acgcgcactg gctcgaggac cagcaccagt ggctcacgcc ggagcagcgg
     1381 cgcctgccgc gcatctacct cgagcacgat ccgccgcgcg agagcccgac cgacgcgcac
     1441 catccggtcg acgatcccgg cgtgctgctc gtgcacgtca cgccgttcaa cgcgctgatg
     1501 tgggacagcg gccgcacgcc gacgcgcgtc atcgagcacg gcgtgatcgc gcctccgcgc
     1561 gccctgtaca ccggcgacta tgcgcgcggg atcgtgatcg tgaacaacct cgcccggcgc
     1621 gggcgcaggc tcggctcgga catcttcaag gccgcgcgcg cgcaggtgcc gctcgacctg
     1681 atcggcatcg gagcggagga gctcggcgga atcggcgagg tcccgcaccg cgacatcccg
     1741 gcgttcgcgg cgcgctaccg gttcctgttc aatcccatcc gctggaccag catggggctc
     1801 gcggtgatcg aggcgatgat ggcgggcgtc ccggtcgtcg ggctcgcgac caccgagatg
     1861 gcgcgcgcga tcgagaacgg cgtgtcggga tgggtcgaca ccagcccggc gcgcctcgtc
     1921 gagcgcatgc gcgttctgct cgacgaccgc aatctcgcgc gcgcgctcgg cgccggcgcc
     1981 cgccggcgcg cgatcgaacg cttcggcatc gagcgcttcg tgcgcgactg gaacgcggcc
     2041 ttcgcgcagg tcaccggcga ccgcgctccg gtcgcggagg gctcggtcgc gcacgcggcg
     2101 tgagcacgcc ggcggtgaag ctcaacctgg gctgcggtcc gaacgtcgcg ccggggtggc
     2161 tcaacctcga cgtcgagccg gtcgcgggcg cggtgcaggg cgacctgcgc gcggggctgc
     2221 cgctcgcgga ctcgagcgtc gactgcatcg ccgcgatcca cctcctccag gatctgccct
     2281 ggcccgaggt ccggtccgcg ctcggcgaga tgcgccgcgt gctgaagccc ggcggcgtgc
     2341 tgcggctcgc cgtgcccgac ctcgaccgcg cgctgcgcgc ctatctcgcc ggcgaccggg
     2401 cgtactggta cgtgcccgac gagcacgcga agagcggcgg cgcgaagctc gtgacgcaga
     2461 tcgtctggta cggctcggtg cgcacgccct gcaactggga cttcgtgcgc gaatggctgc
     2521 tcgccgcgcg ctaccgcgag gtgcggcgct gcgccttcgg cgtgacgcac gcgcccttcc
     2581 ccgagctcgc gcgcctcgac aaccgcgagc gcgagagcct gttcgtcgag gcggtgcgat
     2641 gagcggcgcg ctggtggtct cgccacacct cgacgacgcg gtcctcgcct gcggcgcctg
     2701 gatcgcggcg cacgcgggcg tgcgcgtcct cacggtgttc gccggcgcgc cgcgcacgag
     2761 cgcgccgccg accgagtggg acgccgcgtg cggcttcgcg gacgcggccg aggcgatcgc
     2821 ggcgcgccgc gccgaggacc gcgccgcgct cgcgctgctc ggcgccgagc ccgaatggct
     2881 cgactggtgc gacgaccagt acggcgttcc cgcgcccgaa ggcgcgctcg tcgcggacgt
     2941 gagtgcggac ctgcggcgcg cgctcgagcg ccacgatccg cacgcggtgc tcgtgccgct
     3001 cggcctgttc caccgcgatc acgagcgcac gcacgacgcg gggctcgcgc tcctggagga
     3061 gcaccgcacc cggacctggt acgcgtacga ggacgcgctc taccgcgcga tacccggcct
     3121 ggtgcagcgg cggctccgcc ggctcgcgga gcggcgcgtc acggcgacgc ccgagccgac
     3181 gcccgaacgg acccgtggcg cgagcggccg caagcggcgc gcgctcgcct gctacgcgag
     3241 ccagctgcgc ggcctcgcca cccccggccg ccccgggcac gccgacgcgc tcgcggccga
     3301 acgcctgtgg cgactgacgc tgtagggcgc accgccggcg cgtgcgtctc ggtcgtcatc
     3361 ctgacgcaca accgcgcgcc cgagctggcg ggcacgctcg cccgcacgct ggcgctgccc
     3421 gagcgccccg cggtgatcgt cgtcgacaac ggctcgcgcg acggcacggc cgacctggtg
     3481 gccgcgcgct tcccccaggc gcgcctcgtc cggctctccg acaactacgg cgccgcggcg
     3541 cgcaacgtcg gcgcgcgcgc ggcgcgcacg ccctacgtcg cgttctgcga cgacgacacc
     3601 tgctggcaga gcggatcgct cgcgctcgcc gagcggcttc tcgacgcgca tccgcgcgtc
     3661 gcggtgctgt cggcgcgggt gctcgtcggt gccgagggcc gcgaggaccc ggcgtgcgcg
     3721 gccatggcgg cgagcccgct gccctcggcg ggcctgccgg ggcgctcggt cgtgggcttc
     3781 ctcgccgggg cgtgcgtgtt ccgccgcgac gccttcctcg aggccggggg ctacgagccg
     3841 cgcttcttca tcggcggcga ggaagcgctg ctcgcctacg acctgctcgc gcgcggctgg
     3901 cgcatcgtct atagcgagcg gctcctcgtg cgccatcatc cctcgtcgta ccgcgactcg
     3961 cgccggcgcc gccggctgct cgcgcgcaac gccctgtggg tcgcgtggct gcggcgtccg
     4021 ctgcccgccg cgctcgcgcg cacggcgcgc gtgctgctct cgggaatccc cgaccgcgcg
     4081 gcgatcgccg gttgcatcga cgcgctgcgc gggctgccgt gggtgctgcg caaccggcgc
     4141 gtggtcccgc cgcacgtcga agcgctcagg cgcctggccg acgccggcga atagaagggc
     4201 gcggcccttc ggccgcgccc tcgattggca cgcctgcgcg gggaagctag cccttcttcg
     4261 acccgccctt ggcgccgacg tcggccgcga tctgctgcgc ctggttcagg tgctcctgca
     4321 ccacctgcgc cgtcctctcg gcgaaggctt tcacgtcggg atccttcgcg ttcttcgctt
     4381 ccttctgcag ctccttcagg tcggcgcggt ggtccttcac catctgctgc atgtaggccc
     4441 tgtcgaagtc ggcgcccgac ttcttgtcga ggctcttcgc ctcgcgctcg tgcttgcggt
     4501 cggcggtgtc ggcgaggtcg acgcccttgg attgcgcgag ctgcttcagc tcgtcggccg
     4561 cctggccgtg atcctgaacc atgcgctcgc cgaactgctt gacctggtcg ttcgagccct
     4621 tgctcgaggc gagcttgccg gcctcgatct cggccgcgtt gtcctgcgcg accttctcca
     4681 tgaacttgcg gtcgcgccgc gcgagcttgt cagaggcatc gtccctgccg ccccggttgt
     4741 cgcgcgcgag gttctgcggc gtcgggcgct gttccgactg cgcctggttc tgggcgtccg
     4801 tggacggcga ctgcgtttgc tgcgcgtagc ccgcgccgca ccaggtggcg gcggcgaggc
     4861 ccgtgatggc gagagtgctg gcgatacgag tgcgggtcat gaaatgtcct tccgtgaaac
     4921 gtgcatcgtg aaacggcaat accgcagcaa ggaccatacc gataggacgt cgcccgcgcg
     4981 ccgcagcgcc aggcgtgcgt gcgttgtaaa aacgcgcgag ccctcgcgta attcttacgc
     5041 gatcgaacgc gctttgcgcc gatcggatca tgtcgcgcga ccgcgacggg cgggcaaccg
     5101 cgatgcgcgg atgcagtcca acgtgcagcc aagcccgcat ccatgcccga agcccaagcc
     5161 accgataccc tgttcgagat cggcgagaac tgctgcgcgg tcaagcgcag caatcgtgtc
     5221 gcgctgctcg tcgacgccga gagctatttc gaggcgttca tgcgcgccgc cgagcgcgcc
     5281 gagcgctcga tcgtcgtgct cgcgtgggat ttcaacagcc gcacgccgct gcgcatcggg
     5341 ccggacggcg agcgcctgct gctgggcgat ttcctgaacg gcctcgcgcg ccgccgccgc
     5401 cggctgcacg tgcgcgtgct cgattgggac tatcccatgg tgttcggggt cgaccgcgag
     5461 cttcctccgc tctacggtct gtcgtggacg ccgcaccggc gcgtgcacct gcgctacgac
     5521 ggcacgcatc cgctcgcggg ctcgcaccac cagaagatcg tcgtcatcga cgaccggctc
     5581 gccttcgtcg gaggcctcga tctcaccgcg cggcgctggg acacttcggg ccacgcgccc
     5641 ggcgatccgc gccgcatcgc gagcggcaag gcctatccgc cgttccacga cgtcatgatc
     5701 gcggtcgacg gcgaggccgc ggcgtcgctg gccgagatcg cgcgcaagcg ctggcgtctc
     5761 gcgacccatt cggacattcc gcgcgtcgcg cgcgcgggcg acccgtggcc cgattcgctg
     5821 cgcccggaca tgaccgacgt cgacgtcggc gtcgcgtgca ccgcgccgga gacgagcggc
     5881 gagaagggcg tgcgcgacgt cgagcgcctc tacctcgaca tgatcgcgcg cgcgcggcgc
     5941 tcgatctacg tcgagaacca gtacttcacc tcgcacacga tcggagaagc gctcgccgcc
     6001 cggctcgccg agcccgacgg cccggagatc gtgatcgtga cccgcctcct cagccacggc
     6061 tggctcgaag agatgacgat gcaggtgctg cgcacccgcc tcatccaggc gctgaagcaa
     6121 gtcgaccgct accagcactt ccacgtttac taccccgaca tcgacgggct cgccgaaggc
     6181 acgtgcatcg acgtgcactc gaagctgatg gtcgtcgacg acgagtggct gcgcgtcggc
     6241 tcggcgaacc tctcgaaccg ctcgatgggt ctggacaccg agtgcgacgt cgtggtcgag
     6301 gccggagcgc ggcccgagct gcgccgcgcg gtcagcgctt tccgcgaccg gctgctcgcc
     6361 gagcacaccg gcgccgaagc cgcggacgtc gcgcgcgaga tcgaacgctc gggcggcatg
     6421 gccgcggcgg tcgcgaagct ctcgacgggt ccgcgccggc tgcgccccct cgacgacctc
     6481 aaggactggc ccgaggccgt ggtcgaggcg atcgcggtgg ccgacccgga gcggccggtg
     6541 tcgctcgacc agctggccga cgagttcgcg caggacgagg tcgtgcgcga gcgccggcac
     6601 gtgggccgca acgcggcgat cgtcgccttc gtcctgctcg cgctcgggct cgcatggcgc
     6661 tacacgccgc tcgccgaggt cgcgacgccc gaagcggtgc tcgattggtt cgaccgcttc
     6721 tccggtcact ggtgggcgcc gctcgcgctg ctggccgcct acacgccggc gagcctggtg
     6781 atgttcccgc ggccgctgat cacgctcgcc acggtgatcg cgtacggacc gtggaccgcg
     6841 ttcgcgttct cgatcgcggg caacgtcatc gcggcttgcg tcgtgtatgc ggccggccgc
     6901 aagttcggcc gcgacaccgt gcgccgcatc gcgggcgagc gcctgaaccg cctgtgcagt
     6961 ttgctccagc ggcggggcct gctcgccgtg gtcgcggtgc gcctggtgcc ggtcgcgccg
     7021 ttcgtggtcg agaacgtggt gggcggcgcc gtgcgcatcc gcttctggca gttcgccgtc
     7081 ggcacggcga tcgggcacgt cccgggcacg ctcgcgacga cggtcttcgg ctcgcagatc
     7141 gaggcggcgc tgcgcgagcc gggcaacatc aactggtggc tggtcggcgc cgccacggtg
     7201 ctgctcggcg cggcgatcct cgcgatgcgg cgctggctcg ggcgcctcga gtcccgggaa
     7261 aaggcccgcg agcccgtgcg ccgccgggcg gcggagcggg cgctgctcgc gcgccagcac
     7321 gcggagctcg gctcgtgagc ccgggcgccg gcactccgga gcccgggcgc ggtcgaagcc
     7381 ccggggggag ctccgaggac cgcatgcacc tggtcgacac gacgatgttc ttcgccgcgc
     7441 gcgcgggcgg cgtgcagcgc tatctgtcgg cgaagcgggc gtggctgcgc cgccagggcg
     7501 ccgtgcgcca caccatcgtc gcgccgggcg cggccgacgg cgaggcgcag cccgggatca
     7561 cgacgctgcg cacgcatccg cgcccgcgcc cctacggatt tccgtttccg ctgcggctgc
     7621 gcgcctggcg gcgcgcgctg gtgcggctcg cgcccgacct gatcgaggcc ggcgatccgt
     7681 tcaccccggc gtgggcggcg ctgcgcgcgg gtcagcagct cggcgtcccg gcgatcggct
     7741 tctaccactc ggacctcgtg cgcctggtcg gcacgcggct cggacccgcc gggcaatcgg
     7801 cggccgcgcg ctacgtgaag cagctctacc gcgagttcga cctggtgctc gcgccctcgc
     7861 gctacgtcgc cgcgaggctg cgcgagctgg ggctcgagcg cgtcgtgcac cagccgctcg
     7921 gcgtcgacgc ctcggtgttc catcccgcgc gccgcgaccc gggcttgcgg cgcgcgctcg
     7981 gcctgcccga gcgcacgcgg ctgctcgtgt tcgccggccg cttcgcgcac gagaagaacc
     8041 tgccgctgct gctcgcggcg ttccgcaggc tcgggccccg ctatcacctg ctgctcgtcg
     8101 gcggcggcat ggagctcggg tcgcagcgca acgtcaccgt gctcgatttc cagacgcgcg
     8161 aggccgagct cgcccggctg gtcgcgagct gcgacgcgct ggtgcacgcc ggggagcagg
     8221 agaccttcgg gctggtggtg ctcgaggcga tggcctgcgg gcttccggtc gtcggcgtcg
     8281 ctgcgggcgg cgtcgccgag ctggtcggcg agcggcacgg cgtgctggtg cggccgggca
     8341 gtgcaacggc gctcgccgaa ggcatcgagg cggcgtactc ggcggacgtc gccgtcctcg
     8401 gcgcgcgcgc acgcgaggcg gtcgagcgcc gttacagctg ggattgcgtg atgcagtcgc
     8461 tgctcgggat ctaccaccag cgactgctga tctcgagcgc cgacggcgtg ggcgaggcct
     8521 atgcgctccg ctgagcgagc cacggtctgc atctcggtgc acgatgtcgc gcccgcgacc
     8581 tggccgcgct gcgcgcgcct gctcgcgatg ctcgacgcgc tgggcgccgg gccggtgacg
     8641 ctgctggtcg tgcccgacta ccaccacggc ggccgcatcg acctctatcc ctggttcttg
     8701 cgcgcgatcg aggagcgccg cgcgcgcggc gacgagatcg cgctgcacgg ctactaccac
     8761 gaggacgagg cggcgccgcc gcgcggaccg ctcggctgga tcgagcgccg cgtgctcacg
     8821 cagagcgaag gcgagttcgc cgcgctcggc gccgacgaag cgctcgtgcg cctgcagcgc
     8881 ggcgccgcgc tgatggagtc gctcggctgg accgtgcgcg gcttcgtcgc gcccgcgtgg
     8941 ctgccggggc gcggctgccg cgtcgcgcta tcccggatga cgagcggact cgcgggcttc
     9001 gcgtacacga cgacgcgcag cggcgtctac cgccttcccg actggcgcta caccttctcg
     9061 ccgagcctcg tatgcaccgt gcgcagcgcg tggcgccgct cgatgtcgca gtcgctgaac
     9121 gcggcgagct tcgcgctcgc gagccgcggc ccgctgctgc ggctgtcgct gcacccggtc
     9181 gacgccgagc acgcggacgt gatggcgcgc tggcgcgact ggatcgcgcg cgcgctcgag
     9241 catcacgcgc ccgcgaccaa gctgcaatgg atcgccgccg aaggcttcgt cgcgtccgcc
     9301 gcgtcgtagc gcgtggcatg ttttctgcat cggtgagcga cgtttccgat tccgggagca
     9361 tccgacatgg acaacgagaa gtctccgtcc gacgacagtc aagcaggcag caccgagcgc
     9421 aagccggcgc gtcgcgcgca gccgggcgcg gcagggctcg ggcgcggcgg cgccgacggc
     9481 cgccgctcga gcgacacgct gaagcgcaac caggagcgcc tcggcgtcgg ccccgaccat
     9541 cgcaccgacg cgatgaaaaa aggcaagcgc gggacgtttc cctgagcgcg ggcccgggaa
     9601 gtcttacacc gggcctgcag cttttaccgg gaacgagccc ggcgtcaacc ggaaatatcc
     9661 tctcggcgac gcggccgcct ggcgctcgtt gcggcgcctg ctcgtcgaga cgttcgcgga
     9721 ggcgctcttc gcgggcgcgg cggaagcggc tgggcccgcg cgtcgcgggc gaggcgcttg
     9781 acgaaccgca tcacgagcgt gcgcgccgct tgaccacggc tcgcaggcac atcgccacgg
     9841 cgcccgagag cgcctcgtcg aactcgggct cggcgccgct cacgcccacg gcgatgccgt
     9901 ccacgcagac gctgccccac agcggcgtgt cgtcgcgccg aagcaggtgc ggcgagagcg
     9961 cgcgcaccgc gtgcgagtcg cgaccggtgc gccaggcgag cctcgccttg gcgcgcgcgt
    10021 agcctgcgta gtcggcgtcc cattcggcgc gcggcttgcc gaatccctgc tcggcgagga
    10081 tcgcctgctc gaactcgcag gagcgcccgc acgaacatgc gggccgcgac gggtccatca
    10141 ccacgacgta gacgacgccg ctctggccga cgcccgcgtc gcccgcggcg cgctcgagca
    10201 gcggcgcggc gaggcgcagc gcctcgtgcg ccgcgtcgcg gtcgacgaac agccccggcg
    10261 tgtcgagccc gcgcttcatg ctgcgtagag attaatcgcg atctgcaaag gaacgcctcg
    10321 cgctacgtag gccgaccggc gatcgcgcgc cgcggcttcg cgccgatcag cgcgcccgcg
    10381 ctcgcgatca gctcgcgcgg ctcgaccggc ttcgcgaggt gcatctggaa cccggcgagc
    10441 agcgcctgcg tgcggtcctc gggccgcgcg tagccggtga gcgcgatcgc cggcatgcgc
    10501 tcgggcagcg gcacgccgcg ctcggcctcg agcgcgcgca ggccgtggac cacggcgtag
    10561 ccgtcctcgt cgggcaggcc gatgtcgcac acgaggagat ggggccaggc ctcgtgcggc
    10621 gtgcgcgtga accagccgcg cgcctcgccg ccgcgcgcga aggcgcgcac gtcggcgcct
    10681 ttcgcctcga gcacggtcgc gagcagctcg cgcgcgtcgt cgtcgtcgtc gacgatcgcg
    10741 atggaggcgc cgtccagctt ggggatcgtc gcgccgccac gcgcgcgggg tgttccgtcg
    10801 ggcggcggcg gcagctcggc cgtgccggcg acggcgctca cgggcaggtc gacgacgtag
    10861 cccgtgccgc cgcccgcggg cagccgctcc accgcgaagc gaccgccgtg tcgctcgacc
    10921 gcctcgtgca ggcgcgcgac gtccagccgc agcgctccct cggcgcgctt cggctgcggg
    10981 ccggtgtcga ccaccttgag ccgcacggtc ggcccgttgc ggcgcacctc gatctcgacc
    11041 cggccgcccg cgggcgtgac cgcgatcgcg ttcgcgcaca ggtcccacac catctgctgc
    11101 agcagcgcgg ggtcgccggt gacgcgctcg ctctgcgagc cgatcatcgc gtagaggcgg
    11161 atgcccttcg cgtcgatctc ggggcgcagc acgtcgaccg cggaggccgc gaccgccgcg
    11221 aggttcaccg cctgcagcgc gagacgccgc tcggcgtgcg cgagctcgcg ctcctgctgc
    11281 tggatcgccc acatgcgagc gtagatcccg cggcgctcga gcagctcgtg gtgcgtgccg
    11341 cgctcgacca cgcgcccgtg ctcgagcacg aggatgtcgt cggcgtggac gatggtcgac
    11401 atgcggtgcg cgatgatgag cgtggtgcgg ttctgcgcga cgcgctcgag ctcggcctgg
    11461 atcgcgcgct cggccaccgg gtcgagcgcc gaggtcgcct cgtcgaagat caggatcggc
    11521 gggttcttca ggaccgcgcg cgcgatcgcg atgcgctgct tctcgccccc ggagagcttg
    11581 acgccccgct cgccgacgac cgtctcgtac tgctcgggca gcgccaggat gaagtcgtgc
    11641 agctgcgcgg ccttcgccgc ctcgaccacc tcggcgaagc tcgcgccgga gcgcccgtag
    11701 gcgatgttgt acccgatggt atcgttgaag aggatggtgt cctgcggcac gatgccgatc
    11761 gcgtcgcgca ggctcttcag cgacaccgag cgcacgtcct ggccgtcgat gtacaccccg
    11821 cccgaggcga cgtcgtagaa gcgcagcagc agtcgcgcga gcgtcgactt gcccgagccc
    11881 gagccgccga cgaccgccac ggtcgcgccc ggcggcacgc ggaagtgcac gtcgcgaagc
    11941 acctggcgcg ccggctcgta gccgaagctc acgtgctcga agcgcacctc ggcctcgcgc
    12001 accgcgagcg cgggcgcccc cggcgcctcc tccatgtcgg gccgctgctt gagcagcgcg
    12061 aaaagcttct cggcattgac gacggcgtcg cgcgcctgcc gaaagacgaa gccgagcgcg
    12121 ttcagcggca ggcacacctg gatcacgtag gcgttcacca gcaccaggtc gccgacggtc
    12181 atcgtcccgc gcaccacgtc gtgccccgcg agcagcatga tcgccgcgac gccgaccgcg
    12241 atgatcgcgc tctggccgac gtgcagcatc gacagcgcct tctggttgcg cacgccgacc
    12301 tcgacccact cgcgcatgat cgagcggaag cgcgactgct cgagcgactc gttcgcgtag
    12361 tacttgaccg tgtcgtagtt gagcaggctg tcgaccaggc gcgcgttcga gcgcgagtcg
    12421 agctcgttca tgcgccgctg gtgcacctgg cgccgctcgg tgaacaccag cgtgaacgcc
    12481 gtgtagacgg cgaaggtcgc cgagatgatc gccgcgaacc agctgctgta gttcaggacc
    12541 atgatcgtca tcacggcggc gatctcgacc agcgtcggca cgatcgtgaa caggcccacg
    12601 ccgagcagga atccgatcgc gagcgtgccg cgctcgacct ccctgatcag gcgcccggtc
    12661 tcgcggccgc cgtggaagcg cggcgtgagc gcgagcaggt gcgcgaaggc gcgcgcggtg
    12721 aagtcggacg aggtctgcgc gccgacgcgc gcgaacacga ggtcgcgcag ctcgccgaag
    12781 agcgtccccg cgaagcgcag cagcgcgtag ccgagcagca ggaagacggg cagcacggcg
    12841 acgttctcgg gacgcgagag cgcgtcgacg atgcgcttga gaacgagcgg caccgagacg
    12901 atcgagagct tggcgacgac gagcaggacg atcgccgcga gcacgcgcca gcggaagcgc
    12961 cacgcgcacg accacagcga ggcgagaccg gtggcgcggt tggcgggttc ttccataaag
    13021 actcggctat tgcatcagcg cggtgcctcc tcgaggctct cgcgccggcg ccgattgtcg
    13081 aagccggcgc gccgcgtcct gaggcgctgc tcgaagtagg tgatggtgcg cgcgaggcct
    13141 tcgcgcagcg gaacgcgcgg ctcccagccg agcgccgcgc gcgcgagccc gatatcgggg
    13201 cagcgccgcg cggggtcgtc ggcgggcagc ttcgcatgca cgatgcgcga ccgcgagccg
    13261 gtgagctcga tcaccgtgtc ggcgagctcg gcgatggtga actcgtgcgg gtttcccagg
    13321 ttgacgggtc cgatgaagtc gtccgcgctc gccatcaggc gcacgaccgc gtccaccagg
    13381 tcgctcacgt agcagaacgc gcgcgtctgc gcgccgtcgc cgtagaccct gatcggctcg
    13441 tcctgcagcg cctgcacgac gaagttcgac accacgcggc cgtcgttcgg gtgcatgcgc
    13501 gggccgtagg tgttgaagat gcgcgcgatc ttggtcgcga ggccgtgctg gcgccggtag
    13561 tccatgaaca gcgtctcggc gcagcgcttg ccctcgtcgt agcaggagcg caggcccacc
    13621 gggttgacgt gcccccagta ggcctcgtcc tgcgggtgca tctccgggtc gccgtagacc
    13681 tcgctcgtcg acgcctgaag gatcttggcc ttcgcgcgct tcgccaggcc cagcatgttg
    13741 atcgcgccgt ggacgctggt cttggtcgtc tgcaccgggt cgagctggta gtggatcggc
    13801 gaggcgggac aggcgaagtt gtagatctcg tccacctcga cgtggagcgg gaacgtcacg
    13861 tcgtgtcgca gcagctcgaa gtgcgggtcc tgcgcgaggt gcgcgacgtt gtccttggtg
    13921 ccggtgtaga agttgtcgac gcagagcacg tcgcagccgt cgtcgagcag ccgctcgcac
    13981 aggtgcgagc cgaggaagcc ggcgcccccg gtcacgagca cacgccggcg cagcaggtct
    14041 ctcacggatg gacctccgcg gcccgcgcgc ccgcgcggcg cgcgccggcg ggaaatttcg
    14101 cgagcagcgc gcgcgtctcg gcgatcacgc tctcgggcgc gacgccttgc gcgcactcgt
    14161 gcccgtacgg gcaggacgcg tagctgcagg ggcggcacac gagctcgtag gcgagcacgc
    14221 ggtggcgctc gggatcgagc ggcgaccagc gcgcggtatc ggcgccgcac gacaccacga
    14281 cgctcggcgt gcgcgtcgcg ctggcgacgt gcgaaacgcc ggtgtcgttg cacaccagga
    14341 gccgcgcgcg ggcgatcagc gcggcgagcg cgccgagcga ggtgaggccg gtgaggtcga
    14401 tcgcgcgcga gcgcatcgcg cccgcgaccg cgcaggtgac cgcggcctcg ccggccgtgc
    14461 cggtcagcac cacggtgtgg ccctcggcgg cgagcgcgtc ggcgacgcgc gcgaagcgct
    14521 cgggcggcca gcgccgcgac gcgagctgcg cgccggggtg cacgcacacg acctggcccg
    14581 gccggaaccg gaacgcggag gcgagccgcg ccagctcctc gcggtcgacc tcgtcgatcg
    14641 ggaactcgag ctcgatgccc gcgcccggga cgccgaggcg ctcgagcagc cgcaagccgc
    14701 gcagcacctc gtgctcgcgc ggctccc
//
LOCUS       DATDMW010000039        37589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1254558, whole genome shotgun sequence.
ACCESSION   DATDMW010000039 DATDMW010000000
VERSION     DATDMW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 37589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37589
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254558"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /gene="moaA"
                     /locus_tag="VI319_01365"
     CDS             complement(<1..991)
                     /gene="moaA"
                     /locus_tag="VI319_01365"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY6862520.1"
                     /translation="MTERIIRISDARRSKPLPSAGSLDAGRLDTLGRPLRDLRISVTD
                     RCNFRCVYCMPKDVFGPDYAFLPRDELLSFEEIERLARVFRGHGIEKIRLTGGEPLLR
                     RNLERLIAKLAAIAGVELTLTTNGSLLARKARALKAAGLHRVTVSLDSLDDGVFRAMN
                     DADFPLADVLAGIDAAADAGLAPVKINMVVKRGVNEDSILPMARRFRGSGHIVRFIEF
                     MDVGATNGWRMDDVVPSAEIIRRIGAEMPLEPVDANYAGEVAERWRYADGAGEIGVIS
                     SVTQAFCRTCTRARLSTEGMLYTCLFATAGYDLRSMLRSGASDEAIASAISGIW"
     gene            1066..1596
                     /locus_tag="VI319_01370"
     CDS             1066..1596
                     /locus_tag="VI319_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEY6862521.1"
                     /translation="MNIGAYREDSSIPGNASLPAPLPALAVAVVMERRRLANRWRSEV
                     WQPIGVVPDADSQDASPRLLKRDEEREQWLHPGFKLELFHDEAEGYYLNLSSPQPYVF
                     VNWLEEDGTGVPKLVTVSYNEAARQMDGGATVDGVALPVEWLGWLAEYVARHYRPEPK
                     KKRIRPPSFKGARRDE"
     gene            1598..2116
                     /locus_tag="VI319_01375"
     CDS             1598..2116
                     /locus_tag="VI319_01375"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEY6862522.1"
                     /translation="MSADENFLARWSRRKHERRTDEKATERTAEPPRASSPARPPEPV
                     RAAPELPPVDTLKGLASDYRDFLHPSVDESLRRSALKKLFHDPHFNVMDGLDVYIDDY
                     SKPDPVPESMLKELLARHRSLADRAEPDTQERDEPQAPPAAAVADARAAEPPAAPLPE
                     PSAAADEQKKSA"
     gene            2113..4227
                     /locus_tag="VI319_01380"
     CDS             2113..4227
                     /locus_tag="VI319_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY6862523.1"
                     /translation="MSDPDRALKICSCNGTMALDAKALAQALKRKQPIEIHTELCRRE
                     VAAFGDALKHDACTVACTQEAPLFSEIAQEAGSGAELEFVNIREHAGWSAEGAKAMPK
                     IAALLSLAELPDPEPVPSVEYKSGGGLLVVGPGAAALPWAARLAGQLDVAVLVTDAKG
                     AELPSARGHPVWSGSVRSLKGWLGAFEVEWEQRNPIDLELCTRCNACVRACPEHAIDF
                     SYQVDLDKCKSHRACVAACGPVAAIDFERADRARRERFDLVLDLCDEPCLKLADLPQG
                     YLAPGRDPLEQALAAAQLAQLTGEFEKPRFFAYDEKICAHARSGKQGCTQCLDVCSTG
                     AIAPAGDQIRVEPHLCLGCGGCTTVCPSGALTYAYPRASDLGLRLKRLLSVYAAAGGG
                     DACVLLHNIEDSRELIARLGRRAASGANGGGGRGLPARVIPVEVHHAASVGIDVWLAA
                     LSYGASQVVVLATPHESEAYGAAVQKQMGYAQTIVSALGYAGRHFHWIASDDETRLEQ
                     ALWALEPAAAPSKPATFNVAAEKRGTLEFALEHLARLAPAPRQEIALAAGAPWGRVDV
                     DTKACTLCMACVGACPESALLDGRERPMLSFLERNCVQCGLCAQTCPEEAIALVPRLL
                     LSDQAKRPVVLNEAEPFNCVRCGKPFGTRQMIDNMLGKLTGHSMFASGESLKRLQMCA
                     DCRVVDMMENKAETTILDVPKS"
     gene            4274..4816
                     /locus_tag="VI319_01385"
     CDS             4274..4816
                     /locus_tag="VI319_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEY6862524.1"
                     /translation="MLARLFYSGPDAALLGTLARADEVAEDPGSGLAGAWNALVAAAG
                     AMDAEAARVEHDEVFVGVGKAEVTPYASYYLSETGREKILVRLKQQLAEMGLARAEGA
                     HEPEDHFAGLFEVMRHLISDTDDAALQKQKAFFAQFIAPAYGAFCTAISDCPKANFYK
                     RVAEFARHFLDIESECVKVF"
     gene            4902..5111
                     /locus_tag="VI319_01390"
     CDS             4902..5111
                     /locus_tag="VI319_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEY6862525.1"
                     /translation="MSTTKSKTLSRRNFLLAVGAGGAATAAAVVAGQSAKTANPSPAQ
                     SGDKRRGKGYQETAHVRSYYSTTKV"
     gene            5129..8122
                     /locus_tag="VI319_01395"
     CDS             5129..8122
                     /locus_tag="VI319_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY6862526.1"
                     /translation="MLLTRKSTGDSARPVSGRLSRGLNGMLAKTMDRRAFLKRSGIAA
                     GAGAFATQLPFNMIGKAEAADEPWKTGAKIEVKRTVCTHCSVGCAIDAVVQNGVWVRQ
                     EPVFDSPLNLGAHCAKGASVREHGMTEDSHRLKTPMKLVNGKYQKISWDQAVNEIGDR
                     MMAIRKESGPDAMMFIGSSKHSNEQSYLMCKWVRLWGTNNTDHQARICHSTTVSGVAN
                     TWGYGAMTNSYNDMHNSKAALYIGSNAAEAHPVSMLHSLHAKETGCKIMVADPRFTRT
                     AAKADQYVRIRSGTDVAFLFGVLHHIFKNGWEDKKYIHDRVYGMDKVRDEVLKKWTPD
                     KVQEVTGVPEAEVHKVAETMAKNRPSTIVWCMGQTQHSIGNAMVRASCIVQLALGNIG
                     VSGGGANIFRGHDNVQGATDLGPNPDSLPAYYGLAPGSFAHWAKVWNIDLKWLQSQYV
                     NATMMAKLGITVSRWIDGVLEKNENIDQDPNIRGVFYWGHAPNSQTRGLEMQAAMHAL
                     DLLVVVDPYPSATASMAAMPLDEQGAQILKNAGKEEKKDRAVYLLPACTQFETEGSVT
                     ASNRSLQWREKVIEPLFESKPDQTIMYLFAKKLGFADQLVGKLNGKQNIKLKGDEPDI
                     ADITREFNAGSWTIGYTGQSPERLQAHMRNMHVFDVKTLRAKGGKDAKTGYVLDGDYF
                     GLPWPCYGTPELKHPGSHTLYNTHLHMMDGGGCFRANFGVEKDGVNLLAEDGSYSKGS
                     DITTGYPEFDHVLMKKLGWWDELTDEEKKAAEGKNWKTDLSGGIQRVTMKNHGVHPFG
                     NAKARAVVWNFPDAIPLHREPIYSPRADLVEKYPTHDDKKAFWRLPTLYKSLQEKNKD
                     IGKTFPIILTSGRLVEYEGGGEETRSNPWLAELQQENFVEINPKAANDRGIRNGEFVW
                     VKTPTGARLKVRAMVTERVGPDTAFIPFHFSGWWQGKDMLPFYPDGAAPIVRGEAVNT
                     ATTYGYDSVTMMQETKTTVCQIERA"
     gene            8150..8779
                     /gene="fdh3B"
                     /locus_tag="VI319_01400"
     CDS             8150..8779
                     /gene="fdh3B"
                     /locus_tag="VI319_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HEY6862527.1"
                     /translation="MARMKFLCDAERCIECNGCVTACKQENEVPWGVNRRRVVTINDG
                     VIGAEKSISVACMHCSDAPCMAVCPVDCFYRTDEGVVLHDKDLCIGCGYCFYACPFGA
                     PQFPQAGAFGLRGKMDKCTFCAGGPEKDLSREEFVKYGSNRLAQGKLPACAEMCSTKA
                     LLGGDGDVIADIYRTRVLKRGRGSEVWGWGTAYGKPDNPQARPAPGTKS"
     gene            8776..9036
                     /locus_tag="VI319_01405"
     CDS             8776..9036
                     /locus_tag="VI319_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862528.1"
                     /translation="MSRVAKAAAIFAAACLAAGLAGCGEREQVVVYKQGKYQGKPDAK
                     PWDSEPSAAVYTPQQWTKGDQTSWENALKARNLTQNEYNRAE"
     gene            9048..10100
                     /locus_tag="VI319_01410"
     CDS             9048..10100
                     /locus_tag="VI319_01410"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY6862529.1"
                     /translation="MKSIARQALFLFVLGLGLATLAPASLAQQSTPPPEQNPTQAEMD
                     RQQAQPGNNAPVWREVRKGTPQYTSIPGRETTVLIQSYGETWRQLKNFWITPIAGWFV
                     AAIVVIIGLFYAWRGKITVHEPPTGRLIERFTPFERYMHWTVAITFSILGITGLVMML
                     GKYVLLPVIGYTLFGWLAQLSKHLHNFVGPLFALSAAVMIVKWAWDNRPRTGDLRWLL
                     HLGGMVSGRHMPSWRFNAGEKVWFWVGVVVLGIIVSASGFVLNFPNFDQVRAVMIQAN
                     VVHAVGAGLFIAISLGHIYLGTIGLAGAYDAMRHGYVDEAWAREHHEYWYNDIKSGKV
                     RTGTAVGVPAAPQAQH"
     gene            10114..10338
                     /locus_tag="VI319_01415"
     CDS             10114..10338
                     /locus_tag="VI319_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862530.1"
                     /translation="MRGIRFALLALALGLGGTAWAKLPPPSPEAAAKAAEVKQKADEA
                     AKKDAADLAKAQDRTVANYKKNKAAGGQKR"
     gene            10544..10984
                     /locus_tag="VI319_01420"
     CDS             10544..10984
                     /locus_tag="VI319_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6862531.1"
                     /translation="MDELKPVDILLVEDNSADAEMTLRALRKRNLSNQVHWVKDGPEA
                     LDFLFHRGAYGKRPSGNPKIVLLDLKMPKMDGIEVLRKIREDDRTRLVPVVMLTSSAE
                     ENDIVRSYQLGVNSYIVKPVEFDKFVEEVSKLGVYWAIMNRVPS"
     gene            11004..13613
                     /locus_tag="VI319_01425"
     CDS             11004..13613
                     /locus_tag="VI319_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6862532.1"
                     /translation="MEAPIRVLVIEDVAADVELELHELRRFGLRCEGRCVETEPDFRR
                     ELEDFRPHVILSDFSMPHFDGMTALAIARERHAEIPFIFVSGTIGEEYAIRALKNGAT
                     DYVLKTNLVRLPPAVERALQDARDRAAHSRAETELEETRARLQSVVSSLRAVVWSVAV
                     PSRHVLYISPSSSLVYRRTPEEFYDRRVVWSEVIHEADRSRVGAEWAKAMDGGTFDCE
                     YRIFTPDGAVRWVHSRGTPVKDARGAVCRIDGIARDITERHEQALKIAHLSRIHAVLS
                     GINAAMLRTRERQSLFDEACRVAVQAGDFKMAWIGVIDPATLDGRPVAWAGADDEYIS
                     KARLSARADASEGGRPASRALRTASPVICDDLETEPSVADRREDMLARGYRSLGAFPI
                     PLDGRPHAVLVLYAGDPGFFDDEEVSLLKELAADISFALDNIGREEKLNYLAYYDTLT
                     GLANRDLYRERLEQSLRAAKSERRALAVVMFDIERFRYINDTLGRNNADNVLRQLARR
                     LAEAVPDRSDLARINADCFAASFRDVREAELGRFLRDRVFPSLSRPFAVDGEPLRISV
                     RAGISLYPNDGADPEVLMRNADAALQDAKTRNEHYSFYAPDMNSRVAVNLVLENKLRS
                     ALEKREFVLYYQPKVDLDSGRVVGLEALMRWNDRDQGLVHPLTFIPLLEETGLILEVG
                     AWALEQAATDYRALSESVADCPRIAVNVSPLQLRQKDFAAAVGAAVRADGHALGLDIE
                     ITESVVMDHIENNIAKLKAIRDMGVGIAIDDFGTGYSSLAYISRLPVNTLKIDRSFVH
                     TMARNAEDQSIVSSIISLAHSLRLKVVAEGVETQEQRALLRSLGCDEMQGFLLAPAVP
                     IGRVRDLLRTGRS"
     gene            13733..14083
                     /locus_tag="VI319_01430"
     CDS             13733..14083
                     /locus_tag="VI319_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6862533.1"
                     /translation="MIKARVELHLRVVKGRRRAVGPGKIALLEAIADTGSISAAARKL
                     GMSYRRAWVLVDDLNRQLPRAVVQAVPGGRHGGGTILSPTGHRLVALYRRIERKALRL
                     TAAETRSLVSLLSK"
     gene            14204..14914
                     /gene="modA"
                     /locus_tag="VI319_01435"
     CDS             14204..14914
                     /gene="modA"
                     /locus_tag="VI319_01435"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF007958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6862534.1"
                     /translation="MLALAGSAAAENLTVFAAASLKDALDANVKAYQAKSGDRIAVSY
                     AASSALAKQIEAGAPADLFLSADLDWMDYLEQRKLVRAGTRRNLLRNHLVLIAPAGSN
                     LSVNIAPGFPVARLLGGGRLAMANPDSVPAGKYGKAALAALGVWKDVEPKVAAAENVR
                     AALALVSRGEAPLGIVYRTDAAAEPRVEVVGTFPEDTHPPIVYPIAVTASGKPAAAAF
                     EDWLGGAEAHRTFGKYGF"
     gene            14914..15624
                     /gene="modB"
                     /locus_tag="VI319_01440"
     CDS             14914..15624
                     /gene="modB"
                     /locus_tag="VI319_01440"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY6862535.1"
                     /translation="MGLAPEEQEVLLLSLRVAFWSVAVSLPFAVAVAYLLARGNFPGK
                     TLFDAVVHLPLVLPPVVVGYFLLLLFGKRGPVGSLLDEWLGVTLVFRWTGAALAAAVM
                     GFPLMVRAIRLSFETIDRRLEAAASTLGAGRLGVFATVTLPLALPGVLTGILLSFARG
                     LGEFGATITFVSNIPGETRTLPLAIYTYTQVPAGDAPALRLSLIAVALSLAALFVSEV
                     MVRRATRRIMGDDADARH"
     gene            15602..16684
                     /gene="modC"
                     /locus_tag="VI319_01445"
     CDS             15602..16684
                     /gene="modC"
                     /locus_tag="VI319_01445"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEY6862536.1"
                     /translation="MTLTLDIERHLGAFRLEAAFEAGAGITALFGRSGAGKTSLVNAI
                     AGLLRPDRGRIVIGGEPVFDSGAGIDVPTPARRIGYVFQEGRLFPHLSVAQNLRYAGL
                     FARGAPAPQFAHVVELLGLRDLLERRPGNLSGGEKQRVAIGRALLSSPRLLLLDEPLA
                     SLDAHRKNEVMQYIELLRDEIRIPVVYVSHSAEEVVRLADTVVLLAAGRVSAVGGAED
                     VMGRPDLRAATGAFEGGAVIEAKVTAQDMESDVATLAFDGGTLVATNVDALIGEPVRV
                     RIRARDVSIALERPRRISIQNVLEGTIVDVRAGSSGVVDVAIAIGGTTLRSRVTERAS
                     QQLGLAKGLPVYALVKAVSLSRAATG"
     gene            complement(16712..17767)
                     /locus_tag="VI319_01450"
     CDS             complement(16712..17767)
                     /locus_tag="VI319_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY6862537.1"
                     /translation="MLFRLLEAVRKSGSVAQSAQELGFSYRHVWGLIGKWEKAVGKPL
                     VRLERGRGAKLTEFGEKLIWAEQLARSRLEGHIETVQRELEEELRAQFSRGVTSLVAY
                     ASHDMALGHLRALLQKNHSVQLDLQYRGSLESLDALAQGRCDLAGFHLPEGGLETDFF
                     HRRAFKVRDHSLIRCATRTQGLMVARGNPKGIRTLVHLTRPGVRMINRQPGSGSRLEL
                     DQLLREAGIDAETIEGYDSAEFTHLAVAATIASGMCDAGFGIEAAAAQYKLGFIPLLR
                     ERYYLACRNETVKAQPVQLLIEALKGAEFESLAGGLAGYDWAGVGDLLPAETVLAGRT
                     EGTRTFIRPEAKPVRAR"
     gene            18007..18702
                     /locus_tag="VI319_01455"
     CDS             18007..18702
                     /locus_tag="VI319_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6862538.1"
                     /translation="MLDALAAAARLLVQPSPELIEIVVLSLRVSLSAVALATLLGMPL
                     GALIAVSRFPGRRPLIVLLNAFMGLPPVVVGLVFYLLLSRAGPLGSLGLLFTPAAMVI
                     AQTVLIVPIIAALSRQVIEDAWREYEEQLRSLGAHRLDAALTLLWDTRFSLLTTVLAG
                     FGRAAAEVGAVIIVGGNIDGVTRVMTTTIALETSKGDLPLALGLGAILVGIVIVVNAA
                     AYLTKEAAQRRYG"
     gene            18699..19418
                     /locus_tag="VI319_01460"
     CDS             18699..19418
                     /locus_tag="VI319_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6862539.1"
                     /translation="MSSRPILPLRLTGVSFAAGGRNIIDGVSVELGAGPATIILGANG
                     AGKSVLMRIMHGLLQTTSGRVEWSASALASRRQAMVFQRPVMLRRSALANVLYALALA
                     GVERRHRAAIARDALESVGLAALADRPARVLSIGEQQRLALARAWALHPEVLFLDEPT
                     ASLDPTSTREIETVIRAFDAAGTKIVMATHNLGQARRLGDEILFLDRGRLVERSSVET
                     FFARPQTAQAEAFIRGELPWT"
     gene            19415..20245
                     /locus_tag="VI319_01465"
     CDS             19415..20245
                     /locus_tag="VI319_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6862540.1"
                     /translation="MIRRIVIVALLALVGAGALPAFAQQKFITVASTTSTEQSGLFKY
                     LLPIFEKKSGVQVHVVAVGTGQALDIGRRGDADVVFVHARSAEEKFLSEGQGVKRFPV
                     MYNDFVLVGPKADPAKVAGGGDIVAALRKIKAAQAPFVSRGDRSGTHIAELALWKDAD
                     IDIARDKGPWYKDTGQGMGPALNTAASIGAYILSDRGTWISFKNRGDLKIVVQGDKRL
                     FNQYGVMLVSPQKHPNVKKDLGQEFIDWLISPEGQKVIAGYRIDGQQLFFPNADQPGA
                     "
     gene            20295..20789
                     /gene="mobB"
                     /locus_tag="VI319_01470"
     CDS             20295..20789
                     /gene="mobB"
                     /locus_tag="VI319_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEY6862541.1"
                     /translation="MKIFGITGWSGSGKTTLIEQIVPRFVSKGLVVSLIKHAHHSFDV
                     DHPGKDSYRHRQAGCTEVLVSSPNRWVVMHELRGAPEPTLDEQLGRISPCDLLLIEGY
                     KRYPMPKLEVWRSENAKPFLHPEDTNIVAIATDVPPEAGPRRFDLNDHDGIADFVLEF
                     NGLK"
     gene            20786..21991
                     /gene="glp"
                     /locus_tag="VI319_01475"
     CDS             20786..21991
                     /gene="glp"
                     /locus_tag="VI319_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY6862542.1"
                     /translation="MNKGLLSVDEALERLLSGANTVAGRETVPTLEAVGRVLAETQRS
                     TMNVPPMDNSAMDGYAVRRADVRAPGARLKVSQRIAAGGVGAALAPGTAARIFTGAPV
                     PEGADAIVIQEDTAAEGDAVIVNKVPEPGEWIRRVASDIRAGDAILAAGIRLRPQDTG
                     LAASVGIATLPVVGRVRLGLFFTGDELVMPGDPLPPGRIYNSNRFTLNGLARAFDCDV
                     RDYGIVPDSLDATRAVLRRAASECDVIVTSGGVSVGEADFVKPAVEAEGELLMWRIAM
                     KPGRPLAFGRVAKAAFIGLPGNPVSSFVTFLLFVRPFLLKTQGIAHVAPRAIEARADF
                     DWPRPDPRREFLRVKWNARGGLDLYPTQDSAVLTSTAWADGLVDNPAGQAIRAGDGVR
                     FLAYSELYR"
     gene            21988..22245
                     /gene="moaD"
                     /locus_tag="VI319_01480"
     CDS             21988..22245
                     /gene="moaD"
                     /locus_tag="VI319_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEY6862543.1"
                     /translation="MKIKVLYFAGLREQVGSRGEDLDVPPGVTTVAGVRALLQSRGGP
                     WQSALAQSRAVRIAVNQEMAAATTLVRAGDELAFFPPVTGG"
     gene            22248..22718
                     /gene="moaE"
                     /locus_tag="VI319_01485"
     CDS             22248..22718
                     /gene="moaE"
                     /locus_tag="VI319_01485"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEY6862544.1"
                     /translation="MTVRVQTEDFDAGAEIAALRRGNPKIGAVASFIGVARDVNDGDS
                     VSTLTLEHYPGMTEKALEGIVAEARARWDVIELLVVHRVGEIKPTEQIVLVVVAGAHR
                     GEAFAACEFVMDYLKTRAPFWKKEETPKGARWVDARESDDEAAERWAPGKSSAA"
     gene            22733..23116
                     /gene="crcB"
                     /locus_tag="VI319_01490"
     CDS             22733..23116
                     /gene="crcB"
                     /locus_tag="VI319_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY6862545.1"
                     /translation="MSAYGFAAVGIGAALGAWLRWVLGIALNLLVPTLPLGTLAANLI
                     GGYLIGVAVAVFEQNAVLAPEWRLLVITGFLGGLTTYSTFSAESVALLSTGRYGWAAT
                     HTLAHLGGSLAMTALGIASVRLLQN"
     gene            complement(23214..23501)
                     /locus_tag="VI319_01495"
     CDS             complement(23214..23501)
                     /locus_tag="VI319_01495"
                     /inference="COORDINATES: protein motif:HMM:NF023508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HEY6862546.1"
                     /translation="MRTTYPLYEGDGYLRTAARNEKPSGVDTRAIEARARALRGETLK
                     ALVARFGQWLEASLRPGPRRDVEAYLAQSTDHADLERRLRDVERKNQLGYC"
     gene            23659..24555
                     /locus_tag="VI319_01500"
     CDS             23659..24555
                     /locus_tag="VI319_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6862547.1"
                     /translation="MELYQLRTFAAVADTGHITRAAEKLHLSQPAVSAHIKGLEEELG
                     VRLFERNPGGMLLTRAGRDLRGQAEKVLAEVERLRQLAATLKGDIVGRLRVGTASDPE
                     FVRVGEFLHRTVERYPLLELELHQEVSGAALQQVRTGDLDASFYFGDITSPDVAGLRL
                     HDMAYCVVAPAAWGDRVRNADWSDIARLPWIVTPSISTHNRLVRALFSRHGVEPARVV
                     EADQEPVIASLVVSGVGVSLMREPLALQKQRSGEICIWDKARLQTALWFIYQADRSHD
                     PLIEALLGILGEIWQIAPAPSF"
     gene            complement(24552..25868)
                     /locus_tag="VI319_01505"
     CDS             complement(24552..25868)
                     /locus_tag="VI319_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6862548.1"
                     /translation="MAIKFQLEEHIGKYPITRQIGSGATSRVYLARDPFANRDVAVKV
                     FQFDENINEQTARMMRKAFLAEAALAGKLVHPHIVEILDAASEPEYSYLVMEYVKGET
                     LEAHADVASLLPLGKVVEIVFKCIRALDYAFQHGVIHRDIKPGNILLSDSGDAKVSDF
                     GASFQQRLGQETTQITGIGSPAYMSPEQIRMEDVNHQTDIYSLGVVMYRLLTGRLPYE
                     GATTTAGLTYAILNTPAVPVLTLRPDLPPLLDRIVNRAISKDRKERYSNWMEFGKDLS
                     KAFTSLRLAGESVSDSEKYNELRSLPFFQDFDDVELWEVVRIGSWKSFDAGTVIIREA
                     DQGDSFYLLIEGEVAVTLLGKPLNTIKRGGCFGEILYFSDQTGRRSTSITATTAVTAI
                     EIKADALKAASDACQVGFNKAFMRVLIDRLTQANLRLAGRVEAPSR"
     gene            26066..28678
                     /gene="clpB"
                     /locus_tag="VI319_01510"
     CDS             26066..28678
                     /gene="clpB"
                     /locus_tag="VI319_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY6862549.1"
                     /translation="MRFDKLTTKFQQAISDAQSIAVGQDNPFIEPVHLLQGLLQQEDG
                     TTSSLLSRAGVNVARLRESVKQALARLPKVEGQGGEAPISRDLNNLLNVADREAQKRK
                     DQFIASEMFLLALADDKGEAGRLLKENGGNRKAIEQAIEAVRGGESVGSQEAEGQREA
                     LKKYTLDLTERARSGKLDPVIGRDDEIRRVIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPETLKNKRVLSLDMAALLAGAKYRGEFEERLKSVLKELAQDEGQTIVF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVLVEEPSVEATIAILRGLQEKYEVHHGVDITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIKMEIDSKPESMDRLDRRLIQLKIEREAVRKEKDEASQRRLAAIEDEIKR
                     LEREYADLEEVWKAEKAQVQGSQHVKEELEKLKLEMEAAKRKGDWNRMSELQYGKIPQ
                     LEAQLKKEEKGSAAAKPKLVRTQVGAEEIAEVVSRATGIPVSKMMQGERDKLLKMEDR
                     LHERVVGQDEAVRVVADAIRRSRAGLSDPNKPYGSFLFLGPTGVGKTELCKALAGFLF
                     DSEDHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEK
                     AHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVLVMTSNLGSHMIQQMAGDDYGVIKLA
                     VMAEVKTQFRPEFVNRIDEIVVFHALDEKHIKDIAKIQLRYLEQRLAKLEIRLDLSDS
                     ALAELGKTGFDPVYGARPLKRAIQQEIENPLAKQILEGKFGPKDTVRVEYRGNRMQFE
                     KAGAKAAEKAPAHH"
     gene            complement(28695..29663)
                     /locus_tag="VI319_01515"
     CDS             complement(28695..29663)
                     /locus_tag="VI319_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862550.1"
                     /translation="MKSLIAVLALVAGLFCAATDASAQAYPSRPIRIIVPIPPGGAPD
                     ISARVVGQKLSEQMKQPVIVENRPAANGNVAMDQVAKAEPDGYTLGLFADSNVTVNPH
                     IYKNMPLDTMKDLAPVASVVLNQWVLSVNPSLPVKSFQEFIEYAKKANPPLLYASGGN
                     GSQHELAMEMLKQRAGINLVHVPFKGGSPATTATVAGETAAMFSGTSSAGQIRAGKLR
                     ALAVTGPKRSSLFPELPTIGEFYPGYEVVIWLGLFTASGTPKPVIAKLHDEVNKALTD
                     RDVDGRLAKAGGMQANPLTPDQFAQMIRRDYEKYGKLVKDIGIKVE"
     gene            complement(29660..30289)
                     /locus_tag="VI319_01520"
     CDS             complement(29660..30289)
                     /locus_tag="VI319_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY6862551.1"
                     /translation="MLVSRPALLKGACAALLLAALLPAVPAQESARGVFLVARPGLPD
                     PNFSESVVLVARGADAPIGVIVNRPLSLSLAEVLPGERFRRFDQPMRFGGPVAPNALL
                     ALFAAESAPAGVITMAPGLHLAVDPDAIDRLLHEPPATIRFFSGYSGWAPGQLDAEIA
                     RGDWFVLDADAENALRADTSKLWQQLLVRARAVRAQAGTAKTTTRRVTS"
     gene            complement(30309..30926)
                     /locus_tag="VI319_01525"
     CDS             complement(30309..30926)
                     /locus_tag="VI319_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY6862552.1"
                     /translation="MRWIAAAFALLLVMGMAAPGAAQKTEDTGAVILVASPALSDPDY
                     RHTVLIAVPVEGNRHVGIIINRPTQRSLASLFPEHEPSKQVHDPVYLGGPMLRRAIFA
                     MVRTDHNPGSGSIQMMKHLYLATQGTVVDDIIEKTPNDARYFVGYVAWRPGELRQEID
                     RGLWHVLDADEEMVFRKDSGDSLWEELLRRARAITASVRSAPALG"
     gene            complement(30944..31564)
                     /locus_tag="VI319_01530"
     CDS             complement(30944..31564)
                     /locus_tag="VI319_01530"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY6862553.1"
                     /translation="MAPRSERRRCPWCLGSEAYVEYHDREWGVPVHDDRTLFEFLILE
                     GAQAGLSWSTILAKRANYRRAFANFDPRRVAAFDARRVRALLADPGIVRNRLKVQGAV
                     RNARAFLEVQKAFGSFDAYAWRFVGGRPMKRRPRRMADVPARTRESDALSGDLRRRGF
                     TFVGSTICYAFMQAVGMVDDHLRDCWRAARASGSRKRGSSATLAPK"
     gene            complement(31596..33347)
                     /locus_tag="VI319_01535"
     CDS             complement(31596..33347)
                     /locus_tag="VI319_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6862554.1"
                     /translation="MNRNDDKVNILLVDDQPQRLLSYEAILGTLGENLVKARSGTEAL
                     QKLMEADFAAILLDVNMPTMNGFDTAALIHQHPRFEKTPIIFVTAVHVTNLDQLKGYE
                     LGAVDYCYVPIVPEVLRGKVQVLVELYRKRRELERANEALALANTTLQAEKTRELEQA
                     NAELAKTNEVLEREIAERKRFEQELQEADRRKDEFLAMLSHELRNPLAAISNVAHVMK
                     ASPAIDPQLRWARDVLGRQTGHLTRLIDDLLDVSRITRGKVTLRREPIELKSVVAAAV
                     ETTRTLIHQRGHALQVDVPEEAIPVLGDRVRLTQVVDNVLTNAAKYTQEGGRITVALE
                     TLAGMPGEALIRVRDTGVGIAAEMLPNLFQLFRRADDSIARSEGGLGIGLALARGLVE
                     MHGGRIEAFSDGPGKGSEFVIRLPRANGAVETPEPQARPAVPKSERENRQLKVLVVDD
                     SVDSAESMAIILEMSGHNVRKAHNGPDALRTAAEYRPDVVLMDIGMPGMSGHEVAQRM
                     RETPAMRDVVLIAMTGYGRQVDREQSRAAGFDHHLVKPLDFDKLNEVLVASTASGRCA
                     PAFVPPQAGNHTSLGVR"
     gene            complement(33383..>37589)
                     /locus_tag="VI319_01540"
     CDS             complement(33383..>37589)
                     /locus_tag="VI319_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6862555.1"
                     /translation="KKITVDVSGEVLQLKQTINTMVDQLNAFAGEVTRVAREVGTEGK
                     LGGQAQVSGVAGTWKDLTDNVNFMASNLTNQVRNIAEVATAVARGDLSRKITVDVKGE
                     ILELKNTLNTMVDQLNSFAAEVTRVAREVGTEGKLGGQAQVSGVGGTWKDLTDNVNSM
                     ASNLTAQVRNIADVATAIALGDLSRKITVDVRGEILQLKETINTMVDQLNSFAAEVTR
                     VAREVGTEGKLGGQAQVPGVAGTWKDLTDNVNWMASNLTGQVRNIADVATAIARGDLS
                     RKITVDARGEIFELKNTLNTLVDQLNAFAAEVTRVAREVGTEGKLGGQAQVKGVAGTW
                     KDLTDNVNVMAANLTNQVRGIVKVVTAVANGDLAQKLTVESKGEVAALADTINSMTGT
                     LATFADQVTTVAREVGVEGRLGGQANVPGAAGTWKDLTANVNLLAANLTTQVRAIAEV
                     ATAVTKGDLTRSIQVEAEGEVAELKNYINAMIGNLRETTERNTEQDWLKTNLARFGSM
                     MQGQRDLLTVAQMLLSELAPLVNAQQGVMYVMDDSEFPPVLKQLAGYADHVDPGAPRR
                     YRIGDGLVGQCARERQRILLADIAPESILVHAGLVTARPANVIVLPVLFEGQVKAVIE
                     LAALTHFTPSHLAFLEQLTGSIGIVLNTIEATMRTEGLLQQSQQLAAELQAQQKELQQ
                     TNDELAKKARELAAQYEEVERKNREIDQARRAVEEKAAELALTSRYKSEFLANMSHEL
                     RTPLNSILILGQQLAENVEGNLSAKQIEFAKTIHGAGTDLLNLISDILDLSKIESGTV
                     TVESEELPFATLRDTVERNFRHIAESRKLAFSANFDSDLGRTISTDPKRLLQVLKNLL
                     SNAFKFTEQGGVRIDVSIVESGWSPEHPVLGQADKVVRFSVSDTGIGIPPEKQKIIFE
                     AFQQADAGTARKYGGTGLGLAISRELAHLLGGEIRLHSIPGAGSTFMLYLPLHYQGAA
                     APQPAAHRIAEAPVAFQPIVLPAPRTETVPDDRETLQEGDPSILIVEDDPHYARVLLD
                     LARGRGFKGLVAMRGGEALSLARQYRPTAITLDIFLPDMLGWTLLSQLKHDHTTRHIP
                     VQVITVEEERQHGLERGAFAYLNKPLTSDNLETAFDRIKAFASERVRKLLVVEDNPAE
                     QKSVAELIAHDDVDITCVGTGKEALETLRSKPFDCVVLDLRLPDISGFDLLEQIQREE
                     RLRDVPIVVFTGRDLTPDDEARLRKMAKSIVLKDVRSPERLLDETALFLHRVVANLPD
                     GKRKMLETLHETDDALSGKKALVVDDDVRNIFALSSLLERHGMQATIATNGHEAIQLM
                     ETTPDLSLVLMDIMMPEMDGYETIRRIREVPQFRRLPIVALTAKAMKGDREKCLQAGA
                     SDYIAKPVNSGELLSMLRMWLHP"
BASE COUNT         5989 a        12606 c        13066 g         5928 t
ORIGIN      
        1 gccagatgcc cgagatcgcg gaggcgatcg cctcgtcgga ggcgcccgag cgcagcatcg
       61 agcgcaggtc gtagcccgcg gtcgcgaaca ggcaggtgta gagcatgccc tcggtcgaca
      121 gccgcgcgcg cgtgcaggtg cggcagaacg cctgcgtgac cgacgagatc acgccgatct
      181 cgcccgcgcc gtcggcgtag cgccagcgct cggcgacctc gcccgcgtag ttcgcgtcga
      241 cgggctcgag cggcatctct gcgccgatcc gccggatgat ctccgcggac ggcacgacgt
      301 cgtccatgcg ccagccgttg gtcgcgccga cgtccatgaa ctcgatgaag cgcacgatgt
      361 gcccgctgcc gcggaagcgg cgcgccatcg gcaggatcga gtcctcgttc acgccgcgct
      421 tcaccaccat gttgatcttg accggcgcga ggcccgcgtc ggcggccgcg tcgatgccgg
      481 ccagcacgtc ggcgagcggg aaatccgcgt cgttcatcgc gcggaagacg ccgtcgtcga
      541 gcgagtcgag gctgactgtg acgcggtgca ggcccgcggc cttgagcgca cgcgccttgc
      601 gcgcgagcag cgagccgttc gtggtgagcg tcagctcgac gccggcgatc gcggcgagct
      661 tcgcgatcag gcgctcgagg ttccgccgca gcagcggctc gccgccggtc aggcggatct
      721 tctcgatgcc gtggccgcgg aacacgcgcg cgaggcgctc gatctcctcg aacgacagca
      781 gctcgtcgcg cggcaggaac gcgtaatcgg ggccgaagac gtccttcggc atgcagtaga
      841 cgcagcggaa gttgcagcgg tcggtgaccg agatgcgcag gtcgcgcagc ggccggccga
      901 gcgtgtcgag gcggccggcg tccaaagacc ccgcggaagg caggggcttg gaccggcgtg
      961 cgtcgctgat ccggatgatc cgttcggtca tgcggcccgt tcgaaaatga ttcgagatgc
     1021 tcgaggtgat gcgctcggcg tcgccgccgc gtatcattcg cgaatatgaa catcggtgca
     1081 tatcgcgaag attccagtat acccggaaac gcttcgcttc ccgccccgct gcctgcgctg
     1141 gccgtcgcgg tggtgatgga gcggcggcgc ctcgcgaatc gctggcgctc cgaagtctgg
     1201 cagccgatcg gcgtggtgcc cgacgccgat tcgcaggacg catcgccgcg gctgctcaag
     1261 cgcgacgagg agcgcgagca gtggctgcat cccgggttca agctcgagct cttccacgac
     1321 gaggccgagg gctattatct gaatctgagc tcgccgcagc cgtacgtgtt cgtgaactgg
     1381 ctggaggagg acggaacggg cgtgccgaag ctcgtgacgg tgagctacaa cgaggccgcg
     1441 cgccagatgg acggcggcgc gaccgtcgac ggcgtggccc tgccggtcga atggctcggc
     1501 tggctcgccg agtacgtcgc gcgccactat cgtccggagc cgaagaagaa gcgcatccgc
     1561 ccgccgtcgt tcaagggcgc gcggcgcgac gagtagcatg tccgcagacg aaaatttcct
     1621 cgcgcgctgg tcgcgccgca agcacgagcg caggacggac gagaaggcca cggagcgcac
     1681 ggcggagccg ccccgcgcct cgtcgccggc gcggccgccg gagcccgtgc gcgcggcgcc
     1741 cgagctgccg ccggtggaca cactgaaagg gctcgcgtcc gactaccggg atttcctgca
     1801 cccgtcggtc gacgagagcc tgcgccggtc cgcgctgaag aagctcttcc acgacccgca
     1861 cttcaacgtc atggacgggc tcgacgtcta catcgacgac tactccaagc ccgacccggt
     1921 gccggagtcg atgctcaagg agctgctcgc gcggcatcgc tcgctcgccg atcgcgcgga
     1981 gcccgacacg caggagcgcg atgagccgca ggcgccgccc gcagccgcgg tcgccgacgc
     2041 gcgcgccgcc gagccgcccg ccgcgccgct cccggagccg tcggccgcgg ccgacgagca
     2101 aaagaaatcc gcatgagcga ccccgaccgc gcgctcaaga tctgcagctg caacggcacc
     2161 atggcgctcg acgccaaggc gctcgcgcag gccctcaagc gcaagcagcc gatcgagatc
     2221 catacggagc tgtgccggcg cgaggtcgcc gccttcggcg acgcgctgaa gcacgatgcc
     2281 tgcacggtcg cctgcacgca ggaggcgccg ctcttctccg agatcgcgca ggaggcgggc
     2341 tcgggcgccg agctggagtt cgtcaacatc cgcgagcacg ccggctggtc ggcggagggc
     2401 gcgaaggcca tgccgaagat cgccgcgctg ctttcgctcg cggagctgcc cgatcccgag
     2461 ccggtcccga gcgtcgagta caagtcgggc ggcggactgc tggtcgtcgg tccgggcgcg
     2521 gccgcgctcc cgtgggccgc acggctcgcc gggcagctcg acgtcgccgt gctcgtcacc
     2581 gatgcgaagg gggccgagct gccttcggcg cgcggccacc cggtgtggtc gggcagcgtg
     2641 agatcgctga agggctggct cggcgccttc gaggtggaat gggagcagcg caatccgatc
     2701 gacctcgagc tgtgcacccg ctgcaacgcc tgcgtgcgcg cgtgccccga gcacgcgatc
     2761 gatttctcgt accaggtcga cctggacaag tgcaagtcgc accgcgcctg tgtggccgcg
     2821 tgcgggccgg tcgccgcgat cgacttcgag cgggcggacc gcgcgcgcag ggagcgcttc
     2881 gacctcgtgc tcgacctctg cgacgagccg tgcctgaagc tcgccgacct gccgcagggc
     2941 taccttgcgc cgggacgcga tccgctcgag caggcgctcg ccgcggcgca gctcgcgcag
     3001 ctgacgggcg agttcgagaa gccgcgcttc ttcgcgtacg acgagaagat ctgcgcgcac
     3061 gcgcgctcgg gcaagcaggg ctgcacgcag tgcctcgacg tgtgctcgac cggggccatc
     3121 gccccggcag gcgaccagat ccgggtcgag ccgcacctgt gcctgggctg cggcggctgc
     3181 accacggtgt gcccgtcggg cgcgctcacc tacgcctacc cgcgcgcctc cgacctgggc
     3241 ctgcggctca agcgcctgct ctcggtctac gccgccgccg gcggcgggga cgcgtgcgtg
     3301 ctcctgcaca acatcgagga cagccgcgag ctgatcgcga ggctgggacg gcgcgccgcg
     3361 tcgggcgcga acggcggcgg cgggcgtgga ctgcccgcgc gcgtgatccc ggtggaagtg
     3421 caccacgccg cctcggtcgg catcgacgtg tggctcgcgg cgctgagcta cggcgcgagc
     3481 caggtcgtcg tgctcgccac gccgcacgag tcggaggcgt acggagccgc ggtgcagaag
     3541 cagatgggct acgcgcagac catcgtgagc gcgctcggct acgcgggccg gcatttccac
     3601 tggatcgcct cggacgacga gacgcgcctc gagcaggcgc tgtgggcgct cgagcccgcc
     3661 gcggcgccgt cgaagccggc caccttcaac gtggccgccg agaagcgcgg caccctcgag
     3721 ttcgcgctcg agcacctcgc ccggctggcg cccgcgccgc ggcaggagat cgcgctcgcg
     3781 gccggcgcgc cgtgggggcg cgtcgacgtc gacacgaagg cgtgcacgct gtgcatggcc
     3841 tgcgtcggcg cgtgtcccga gtcggcgctg ctcgacggac gcgagcggcc gatgctgagt
     3901 ttcctcgagc gcaactgcgt gcagtgcggg ctgtgcgcgc agacctgccc cgaggaggcg
     3961 atcgcgctgg tcccgcgcct gctgctctcc gaccaggcga agcggccggt ggtgctcaac
     4021 gaggcggagc cgttcaactg cgtgcgctgc ggcaagccct tcggcacgcg ccagatgatc
     4081 gacaacatgc tgggcaagct caccgggcac tcgatgttcg cgagcggcga gtcgctgaag
     4141 cgcctgcaga tgtgcgccga ctgccgcgtc gtcgacatga tggaaaacaa ggccgagacc
     4201 acgatcctcg atgtgcccaa gtcctgaggg ggaaggcgcc gaggaggacc gcgcacgggc
     4261 cggctattac gcgttgctcg cgcgactgtt ctactcgggg ccggacgccg cgctgctcgg
     4321 cacgctcgcg cgggcggacg aggtggcgga ggacccggga tcgggtctcg ccggcgcatg
     4381 gaacgcgctc gtggccgcgg ccggcgcgat ggacgccgag gcggcccggg tcgagcacga
     4441 cgaagtcttc gtcggcgtcg gcaaggccga ggtaacccct tacgccagct actatctgtc
     4501 cgagacgggc cgcgagaaga tcctggtccg gctgaagcag cagctcgccg aaatggggct
     4561 cgctcgtgcc gagggcgcgc acgagccgga ggatcacttc gccggcctgt tcgaggtgat
     4621 gcggcacctg atctcggaca cggacgatgc tgcgctgcaa aaacagaagg cgttcttcgc
     4681 ccagttcatc gctcccgcct atggcgcttt ttgcactgca atatcggatt gcccgaaagc
     4741 gaatttctat aagcgcgtcg cggagttcgc gcgccatttc ttggatattg aaagcgagtg
     4801 cgtaaaggtg ttctaattag gaaatccgcg acatatttca cagatcggtt taaccgacac
     4861 tcaagtattt ttctcgcggc atcggagaag gagatatcgg aatgagcact acaaaatcca
     4921 aaacactttc ccgcagaaac ttccttcttg cagtaggcgc cggcggcgcc gcgacagccg
     4981 ccgccgtcgt ggccggccag tcggccaaga ccgcgaaccc atcacccgca cagagcggcg
     5041 acaagcggcg cggaaagggc tatcaagaga ccgcgcacgt ccgcagctac tacagcacca
     5101 ccaaagtttg aatcgaggag gcgtgaccat gcttctcacc aggaaatcca ctggggattc
     5161 cgctcgcccc gtttccggac gtctgtcccg cggcctcaac ggcatgctgg cgaagacgat
     5221 ggatcgccgc gccttcctga agcgctccgg gatcgccgcg ggcgcaggcg ccttcgcgac
     5281 gcagctgccg ttcaacatga tcggcaaggc cgaagcggcc gacgagccgt ggaagacggg
     5341 agccaagatc gaggtgaagc gcaccgtgtg cacgcactgc tcggtgggct gcgccatcga
     5401 cgccgtcgtg cagaacggcg tgtgggtgcg ccaggagccg gtgttcgact cgccgctcaa
     5461 cctcggcgcg cactgcgcca agggcgcgtc tgtgcgcgag cacgggatga ccgaggactc
     5521 gcaccggctg aagaccccga tgaagctggt gaacggcaag taccagaaga tctcctggga
     5581 ccaggcggtc aacgagatcg gcgaccgcat gatggcgatc cgcaaggagt cggggccgga
     5641 cgcgatgatg ttcatcggct cctccaagca cagcaacgag cagtcttacc tgatgtgcaa
     5701 atgggtgcgc ctctggggca ccaacaacac cgaccaccag gcgcgcattt gccactcgac
     5761 cacggtctcg ggcgtcgcga acacctgggg ctacggcgcg atgaccaact cgtacaacga
     5821 catgcacaac agcaaggcgg cgctctacat cggctcgaac gcggccgagg cgcacccggt
     5881 gtcgatgctg cattcgctgc acgcgaagga gaccggctgc aaaatcatgg tcgccgaccc
     5941 gcgcttcacg cgcaccgcgg ccaaggccga ccagtacgtc cgcatccgct ccgggacgga
     6001 cgtcgcgttc ctgttcggcg tgctgcacca catcttcaag aacggctggg aggacaagaa
     6061 gtacatccac gaccgtgttt acggcatgga caaagtgcgc gacgaggtcc tgaagaaatg
     6121 gacgcccgac aaggtccagg aagtgaccgg cgttcccgag gccgaggtgc acaaggtcgc
     6181 cgagaccatg gccaagaacc ggccgagcac catcgtatgg tgcatgggcc agacgcagca
     6241 ctcgatcggc aacgccatgg tgcgcgcgtc gtgcatcgtg cagctcgcgc tcggcaacat
     6301 cggcgtctcg ggcggcggcg cgaacatctt ccgcggccac gacaacgtgc agggcgcgac
     6361 cgacctgggc ccgaaccccg actcgctccc ggcctactac ggcctggcgc cgggatcgtt
     6421 cgcccactgg gccaaggtct ggaacatcga cctgaagtgg ctgcagagcc agtacgtcaa
     6481 tgcgaccatg atggcgaagc tcggcatcac ggtgtcgcgc tggatcgacg gtgtgctgga
     6541 gaagaacgag aacatcgacc aggatccgaa catccgcggc gtcttctact ggggccatgc
     6601 gccgaacagc cagacgcgcg gcctcgagat gcaggccgcg atgcacgcgc tcgacctgct
     6661 ggtcgtggtc gacccgtacc cgtccgcgac cgcgtcgatg gcggcgatgc ccctcgatga
     6721 gcagggcgcg cagatcctga agaacgcggg caaggaggag aagaaggacc gcgccgtcta
     6781 cctgctcccc gcgtgcacgc agttcgagac cgagggctcg gtgacggcgt cgaaccgctc
     6841 gctgcagtgg cgcgagaagg tgatcgagcc gctgttcgag tcgaagcccg accagaccat
     6901 catgtacctg ttcgcgaaga agctcgggtt cgcggaccag ctggtcggga agctgaacgg
     6961 caagcagaac atcaagctga agggcgacga gcccgatatc gccgacatca cgcgcgagtt
     7021 caacgccggc agctggacca tcggctacac cggccagtcg cccgagcggc tgcaggcgca
     7081 catgcgcaac atgcacgtct tcgacgtgaa gacgctgcgc gcgaagggcg gcaaggacgc
     7141 gaagaccggc tacgtgctcg acggcgacta cttcggcctg ccgtggccgt gctacggcac
     7201 gcccgagctg aagcaccccg gctcgcacac gctgtacaac acgcacctgc acatgatgga
     7261 cggcggcggc tgcttccgcg cgaacttcgg cgtggagaag gacggcgtca acctcctggc
     7321 cgaggacggc tcgtactcca agggcagcga catcaccacc gggtatcccg agttcgacca
     7381 cgtgctgatg aagaagctcg gctggtggga cgagctcacc gacgaggaga agaaggccgc
     7441 cgagggcaag aactggaaga ccgacctttc gggcggcatc cagcgcgtga ccatgaagaa
     7501 ccacggcgtg cacccgttcg gcaacgccaa ggcgcgcgcg gtcgtctgga actttcccga
     7561 cgccatcccg ctgcaccgcg agccgatcta ctcgccgcgt gccgatctcg tcgagaagta
     7621 tccgacccac gacgacaaga aggcgttctg gcgcctgccg acgctgtaca agtcgctgca
     7681 ggagaaaaac aaggacatcg gcaagacgtt cccgatcatc ctgacgagcg ggcggctggt
     7741 ggagtacgag ggcgggggcg aggagacgcg ctccaacccc tggctcgccg agctgcagca
     7801 ggagaacttc gtcgagatca atccgaaggc ggcgaacgac cgcggcatcc gcaacggcga
     7861 gttcgtctgg gtgaagacgc ccacgggcgc gcgcctgaag gtgcgcgcga tggtcaccga
     7921 gcgcgtcggg cccgacaccg cgttcatccc gttccacttc tcgggctggt ggcagggcaa
     7981 ggacatgctg ccgttctacc cggacggggc ggcgccgatc gtgcgcggcg aggcggtcaa
     8041 caccgcgacc acctacggct acgactcggt gacgatgatg caggagacca agaccaccgt
     8101 gtgccagatc gagcgggcat aacggggcga ggggactaag gagaccacta tggccagaat
     8161 gaaattcctc tgcgacgccg agcgctgcat cgagtgcaac ggctgcgtga cggcgtgcaa
     8221 gcaggagaac gaggttccct ggggcgtcaa ccgccgccgc gtcgtgacca tcaacgacgg
     8281 cgtcatcggc gccgagaaat cgatctcggt cgcgtgcatg cattgctccg acgcgccgtg
     8341 catggcggtg tgcccggtcg attgcttcta ccgcaccgac gagggcgtgg tcctgcacga
     8401 caaggacctg tgcatcggct gcggctactg cttctacgcc tgcccgttcg gggcgccgca
     8461 gttcccgcag gcgggcgcct tcggcctgcg cggcaagatg gacaagtgca cgttctgcgc
     8521 cggcggcccc gagaaggacc tgtcgcgcga ggagttcgtc aagtacggct cgaaccgcct
     8581 cgcgcagggc aagttgccgg cgtgtgcgga gatgtgctcg acgaaggcgc ttctgggcgg
     8641 cgacggcgac gtcatcgccg acatctaccg cacgcgcgtg ctcaagcgcg gccgtggctc
     8701 ggaggtctgg ggctggggca ccgcctacgg caagcccgac aatccgcagg cgcgtcccgc
     8761 gccggggacg aaatcatgag ccgcgtcgcg aaggccgcgg cgattttcgc cgctgcgtgt
     8821 ctggccgcgg gcctcgccgg ctgcggcgag cgcgagcagg tcgtcgtcta caagcagggc
     8881 aagtaccagg gcaagcccga cgccaagccc tgggacagcg agccgagcgc cgcggtctac
     8941 acgccgcagc agtggacgaa gggcgaccag acgagctggg agaacgcgct gaaggcgcgc
     9001 aatctcacgc agaacgaata caaccgagcc gagtagaggg cgccttgatg aagtccatcg
     9061 cacggcaagc gctcttcctt ttcgtgttgg ggctgggact cgcgacgctc gcgccggcga
     9121 gcctggctca gcagtcgact ccgccgcccg agcagaaccc gacgcaggcg gagatggacc
     9181 gacagcaggc gcagcccggc aacaatgcgc cggtctggcg cgaggtgcgc aagggcactc
     9241 cgcagtacac ctccatcccg gggcgcgaga ccacggtcct gatccagagc tacggcgaga
     9301 cgtggcggca gctgaagaac ttctggatca cgccgatcgc cggctggttc gtcgcggcga
     9361 tcgtggtcat catcggcctg ttctacgcct ggcgcggcaa gatcacggtg cacgagccgc
     9421 ccacgggccg gctgatcgag cgcttcacgc cgttcgagcg ctacatgcac tggacggtcg
     9481 cgatcacgtt ctcgatcctg ggcatcacgg ggctcgtcat gatgctcgga aagtacgtgc
     9541 tgctgccggt gatcgggtac acgctgttcg gctggctcgc tcagctctcg aagcacctgc
     9601 acaatttcgt cgggccgctg ttcgcgctga gcgcggcggt gatgatcgtc aagtgggcat
     9661 gggacaaccg gccgcgcacg ggcgacttgc gctggctgtt gcacctgggc ggcatggtct
     9721 cggggcgcca tatgccgtcg tggcgcttca acgccggcga gaaagtttgg ttctgggtcg
     9781 gcgtggtggt gctcggtatc atcgtcagtg cgtcgggatt cgtgctgaac ttcccgaatt
     9841 tcgaccaggt ccgcgccgtc atgatccagg ccaacgtcgt gcacgcggtc ggcgccgggc
     9901 tgttcatcgc gatctcgctc ggacacatct atctcggcac gatcgggctc gccggggcct
     9961 acgacgcgat gcgtcacggc tatgtcgacg aggcctgggc cagggaacac cacgagtact
    10021 ggtacaacga catcaaatcg ggcaaggtga ggaccggcac cgccgtcggc gtgcccgccg
    10081 cgccgcaggc gcagcactga ggaggaaatc gacatgaggg gtatccgttt cgctttgctg
    10141 gcgctcgcat tgggtctggg aggcaccgcc tgggcgaagc tcccgccgcc gtccccggaa
    10201 gcggccgcca aggcggccga ggtcaagcag aaggcggacg aggccgcgaa aaaggacgcc
    10261 gcggacctgg ccaaggcgca ggaccggacg gtggcgaact acaagaaaaa caaggccgcg
    10321 gggggccaga agcggtagcc tgtccgggcc ttcacggacg aaaaaggggc gctgcggcgc
    10381 cccttctctt tttcttcccg caatttgccg cgcccgccgg gggcagcccg gggaactttt
    10441 ccggcgttca cgcccttaat gatattaagg tggcaacaag agcgcgcccg gtccccccac
    10501 tggcgccgtt cctgagcgat tttcggaggc ggatggcgca cacatggacg agctgaaacc
    10561 ggtcgacatc cttctcgtcg aggacaattc cgcggacgcg gaaatgacct tgcgcgcgct
    10621 caggaagcgc aatctctcga accaggtcca ctgggtcaag gacggtcccg aggcgctgga
    10681 ctttctgttc caccgcggcg cgtacggaaa acggccgtcg ggcaatccga agatcgtcct
    10741 gctcgacctg aagatgccca agatggacgg catcgaggtc ctgcgcaaga tccgcgagga
    10801 cgaccggacg cggctggtcc cggtcgtcat gctgacctcg agcgcggagg agaacgacat
    10861 cgtccgcagc taccagctcg gggtcaacag ctacatcgtg aagccggtcg agttcgacaa
    10921 gttcgtcgag gaggtctcga agctcggcgt ctactgggcc atcatgaacc gggtcccgtc
    10981 ctgagcgcct gaacgccggc ttcatggaag cgcctatccg ggtcctcgtc atcgaggacg
    11041 tcgccgccga cgtcgagctc gagctgcacg agctcaggcg cttcggcttg cgctgcgagg
    11101 gccgctgcgt cgagaccgag cccgatttcc ggcgcgagct ggaggacttc cgcccccacg
    11161 tgattctttc cgatttttcc atgccgcact tcgacggcat gaccgcgctc gccatcgcgc
    11221 gcgagcggca cgcggagatc cccttcatct tcgtttccgg gaccatcggc gaggaatacg
    11281 cgatccgcgc gctgaagaac ggcgcgacgg actacgtgct gaagacgaac ctcgtgcgcc
    11341 tgccgcccgc ggtcgagcgc gcgctgcagg acgcgcgcga ccgcgcggcg cacagcaggg
    11401 ccgagaccga gctcgaggag acacgcgcgc ggctccagag cgtcgtgtcc tcgctgcgcg
    11461 ccgtggtgtg gtccgtggcc gtcccctcgc gccacgtcct gtacatcagc ccttcgagct
    11521 cgctcgtcta ccggcggacg cccgaggagt tctacgaccg gcgtgtcgtc tggtccgagg
    11581 tcatccacga ggccgaccgc agccgggtcg gcgcggagtg ggccaaagca atggatggcg
    11641 gcacgttcga ttgcgaatac cggatcttca cgcccgacgg agcggtgcgc tgggtgcaca
    11701 gccgcggcac gccggtgaaa gacgcccgcg gagcggtgtg ccgcatcgac ggcatcgcgc
    11761 gcgacatcac cgagcggcac gagcaggcgc tgaagatcgc gcacctcagc cgcatccacg
    11821 cggtgctgag cggcatcaac gcggccatgc tgcgcacccg cgagcgccag agcctcttcg
    11881 acgaagcctg ccgcgtcgcg gtccaggcgg gcgacttcaa gatggcgtgg atcggcgtga
    11941 tcgatcccgc gacgctggac ggcaggccgg ttgcctgggc cggcgcggat gacgagtaca
    12001 tctccaaggc caggctcagc gcgcgcgcgg acgcgtccga aggcgggcgg ccggcgagcc
    12061 gcgcgctgcg gaccgcgagc cccgtgatct gcgacgatct cgagaccgag ccctcggtcg
    12121 cggaccggcg cgaggacatg ctcgcccgag gataccgctc gctcggtgcc ttcccgattc
    12181 cgctcgacgg gcggccgcac gccgtgctgg tcctgtacgc cggagatccc gggttcttcg
    12241 acgatgagga ggtgagcctg ctgaaggagc tcgcggcgga catctcgttc gcgctggaca
    12301 acatcggccg ggaggagaag ctcaactacc tcgcctacta cgacacgctc accggcctcg
    12361 cgaaccgcga tctgtaccgc gagcgcctcg agcagagcct gcgcgcggcg aagtcggaac
    12421 gccgcgcgct cgcggtggtg atgttcgaca tcgagcgctt ccgctacatc aacgacacgc
    12481 tgggacggaa caacgcggac aacgtcctgc gccagctcgc acggcggctc gcggaggcgg
    12541 tacccgaccg cagcgacctc gcgcgcatca acgccgattg cttcgccgcg tcgtttcgcg
    12601 acgttcggga ggccgagctc gggcgcttcc tgcgcgaccg cgtgttcccg tcgctgagcc
    12661 ggcccttcgc ggtcgacggc gagccgttgc gcatctcggt gcgcgccggg atctcgctct
    12721 atccgaacga cggcgccgat cccgaggtcc tgatgcgcaa cgccgatgcc gcgctgcagg
    12781 acgcgaagac gcgcaacgag cactactcgt tctacgcccc ggacatgaac tcacgcgtcg
    12841 cggtgaacct cgtgctcgag aacaagctgc gctcggcgct cgagaagcgc gagttcgttc
    12901 tgtactacca gccgaaggtc gatctcgact cgggccgcgt ggtggggctc gaagccctga
    12961 tgcggtggaa cgaccgcgac cagggactgg tgcacccgct caccttcatc ccgctgctcg
    13021 aggagacggg gctcatcctc gaggttggcg cctgggcgct ggagcaggcc gcgacggact
    13081 accgtgcgct gtccgagtcg gtggccgact gcccccggat cgccgtgaac gtctcgccgc
    13141 tgcagctgcg gcagaaggac ttcgccgcgg cggtgggcgc cgccgtgcgc gccgacggtc
    13201 acgccctcgg cctggacatc gagatcaccg agagcgtggt catggaccac atcgagaaca
    13261 acatcgccaa gctcaaggcg atccgggaca tgggcgtcgg catcgccatc gacgacttcg
    13321 gcaccggcta ctcgtcgctc gcgtacatct ccaggctccc ggtgaacacg ctcaagatcg
    13381 accgctcgtt cgtccatacc atggcgcgca acgcggagga ccagagcatc gtctcgagca
    13441 tcatctcgct cgcgcactcg ctgcgcctga aggtcgtggc cgagggcgtg gagacccagg
    13501 agcagcgcgc gctgctgcgc tcgctcgggt gtgacgagat gcagggcttc ctgctcgcgc
    13561 ccgcggttcc catcggccgg gttcgggacc tgctgcgcac cggccgcagc tagtgcgcga
    13621 ctcccggcgc gccgctcccg cgagcggatc tgcgatcagg tggtgcgcgg cggcgaccgc
    13681 ggcgaagccg atgaaatata ctttcgcgac cgacctgcct gcaaccgccc acatgataaa
    13741 ggcccgagtc gagctccacc tccgcgtcgt caagggtcgc cggcgtgccg tcggcccggg
    13801 gaagatcgcg ctcctcgagg cgatcgccga caccggctcg atctccgccg ccgcgcgcaa
    13861 gctcggcatg tcgtaccggc gcgcgtgggt gctggtcgac gacctgaacc ggcagctccc
    13921 gagggcggtc gtgcaggccg tccccggagg gcggcatggc ggcggcacca tcctctcgcc
    13981 gaccggacac cgcctggtgg cgctctaccg ccgcatcgag cgcaaggcgt tgcggctgac
    14041 cgcggccgag acgcgcagcc tcgtttccct gctttcgaaa tagagcccgc ggccggacgg
    14101 gcggtttggc gagatatcgc tatatatgtc aggatatagc gatagcgggt ggaggaggat
    14161 cgggatgcag acgtggtggt tgcgggtcgc gacgagtttg ggagtgctgg cgctcgcggg
    14221 ctcggcggcc gccgaaaacc tgacggtgtt cgcggcggcg agcctcaagg atgcgctgga
    14281 cgcgaacgtg aaagcgtacc aggcgaagag cggcgaccgg atcgccgtct cttacgccgc
    14341 gagctcggcg ctcgcgaagc agatcgaggc cggcgcgccg gccgatctgt tcctctcggc
    14401 cgatctcgac tggatggact atctcgagca gcgcaagctc gtccgggccg gtacgcgccg
    14461 gaacctgctc aggaatcacc tggtgctgat cgcgcccgcg ggcagcaatc tttccgtaaa
    14521 cattgcgccg gggtttccgg tcgcaagact gctcggcggc gggcgtctcg cgatggccaa
    14581 cccggattcg gtccccgccg gaaaatacgg caaggccgct ctcgcggcgc tcggcgtctg
    14641 gaaggacgtc gaaccgaagg tggcggccgc cgagaacgtg cgcgccgcgt tggcactggt
    14701 gtcgcgcggc gaagcgccgc tcggcatcgt ctatcgcacc gacgcggcgg ccgagccgcg
    14761 cgtcgaggtc gtcgggacgt ttcccgagga cacgcacccg ccgatcgtct atccgatcgc
    14821 cgtgaccgcg tcgggcaagc ccgccgcggc cgcgttcgag gactggctcg gcggcgccga
    14881 ggcgcacagg acgttcggga agtacggctt ctgatggggc tcgcgccgga agagcaggag
    14941 gtgttgctgc tcagcctgcg cgtggcgttc tggagcgtcg ccgtcagcct gcctttcgcg
    15001 gtggcggtgg cctaccttct cgcgcgtggc aattttcccg gcaagacgct cttcgacgca
    15061 gtcgtgcacc tgccgctcgt gctgccgccc gtcgtggtcg ggtacttcct tctgctcctc
    15121 ttcggcaaac gcgggccggt cggctcgctg ctcgacgagt ggctcggcgt gacgctcgtc
    15181 ttccgctgga ccggcgccgc gctcgcggcg gcggtgatgg gcttcccgct gatggtgcgc
    15241 gcgatccgcc tgtcgttcga gacgatcgac cgccggctgg aggctgcggc gagcacgctg
    15301 ggcgcggggc gcctcggcgt gttcgcgacg gtgacgctgc cgctcgcgct ccccggcgtg
    15361 ctcaccggga tcctgctttc gttcgcgcgc ggtctcggtg agttcggcgc cacgatcacc
    15421 ttcgtctcca acatcccggg cgagacccgc acgctgccgc tcgcgatcta cacgtataca
    15481 caggtcccgg cgggcgacgc gcctgcgctg cggctcagcc tgatcgcggt cgcgctgtcg
    15541 ctcgctgcgc tgttcgtttc cgaggtcatg gtgcgccggg ccacgcgcag gatcatgggc
    15601 gatgacgctg acgctcgaca ttgaacggca cctgggcgcc ttccgtctcg aagcggcatt
    15661 cgaggcgggc gcgggcatca cggcgctgtt cgggcgctcg ggcgcgggca agacctcgct
    15721 cgtgaacgcg atcgccggcc tcctgcgccc ggatcgcggc cgcatcgtga tcggcggcga
    15781 gccggtgttc gacagcggcg cgggcatcga cgtgccgacg ccggcgcggc gcatcggcta
    15841 cgtgttccag gaggggcgcc tgtttccgca cctcagcgtg gcgcagaacc tgcgctatgc
    15901 gggactgttc gcgcgcgggg cgccggcgcc gcagttcgcg cacgtggtcg agctgctcgg
    15961 gctcagggac ctgctcgagc gccggccggg caacctgtcg ggcggcgaga agcagcgcgt
    16021 cgcgatcgga cgcgcgctcc tctcgagccc gcggctgctg ctgctcgacg agccgttggc
    16081 ctcgctcgac gcgcaccgca agaacgaggt catgcagtac atcgagctgc tgcgcgacga
    16141 aatccgcatt cctgtggtgt acgtcagcca ctcggcggag gaagtggtgc ggctcgcgga
    16201 caccgtcgtg ctgcttgccg cgggccgggt gagcgcggtg ggcggcgccg aggacgtgat
    16261 gggccggccc gacctgcgcg cggcgacggg cgcattcgag ggcggggcgg tgatcgaagc
    16321 gaaggtgacc gcgcaggaca tggagagcga tgtcgcgacg ctcgccttcg acggcggcac
    16381 gctcgtcgcg acgaacgtcg acgccctgat cggcgagcca gtgcgggtgc gcatccgcgc
    16441 gcgcgatgtc tcgatcgcgc tggagcgccc gcggcgcatc agcatccaga acgtgctcga
    16501 aggcacgatc gtcgacgtgc gcgcgggatc gagcggcgtc gtcgacgtcg cgatcgcgat
    16561 cggcggcacc acgctgcggt cgcgcgtcac ggagcgggca tcgcagcagc tcgggctggc
    16621 gaagggactg ccggtctacg cgctggtgaa agccgtctcg ctttcgcggg cggcgacggg
    16681 ctgacggggc tattcgctta caggagccgc ttcagcgggc gcggaccggt ttggcctcgg
    16741 gtcgaatgaa ggtgcgggtg ccctcggtcc tgcccgcaag cacggtctcg gccggcagca
    16801 gatcgccgac gcccgcccag tcgtagccgg cgagcccgcc ggccagcgat tcgaactcgg
    16861 cgcccttcag cgcctcgatc aggagctgca ccggctgagc tttcacggtc tcgttgcggc
    16921 aggcgaggta gtagcgctcc cgcagcagcg gaatgaagcc gagcttgtat tgcgcggcgg
    16981 cggcctcgat gccgaatccg gcgtcgcaca ttcccgaggc gatcgtcgcc gcgacggcga
    17041 gatgagtgaa ctcggcgctg tcgtatccct cgatggtctc cgcgtcgata cccgcctcgc
    17101 gcagcagctg gtcgagctcg aggcggctgc ccgatccggg ctggcgattg atcatgcgca
    17161 cgcccgggcg cgtgagatgc acgagggtgc gaattccctt gggattgccg cgggcgacca
    17221 tcagcccctg cgtgcgcgtc gcgcagcgga tcagcgaatg gtcgcgcacc ttgaacgcgc
    17281 ggcggtggaa gaagtccgtc tcgaggccgc cctcgggcag gtggaacccg gcgaggtcgc
    17341 agcgtccctg ggcgagcgcg tcgaggctct cgaggctgcc gcggtactgc aggtcgagct
    17401 ggaccgaatg attcttctgc agcagggcgc gcaggtgacc gagcgccatg tcgtggctcg
    17461 cgtacgcgac caggctcgtc acgccccgcg agaattgcgc gcgcagctct tcctcgagct
    17521 cgcgctggac ggtttcgatg tgcccttcga gccgcgagcg cgcgagctgc tccgcccaga
    17581 tgagcttttc gccgaactcg gtgagctttg cgccgcgtcc gcgctcgaga cgcaccagcg
    17641 gtttcccgac ggccttctcc cacttcccga tgaggcccca gacatgccgg tacgagaacc
    17701 cgagctcctg cgccgactgc gcgaccgatc ccgacttgcg gaccgcctcg agaagacgga
    17761 acagcatcgg actcccggac ggggaatcgt cggtcccgat cttccagttc gcttcgagct
    17821 gaatccgaat catttgtgaa atgagacagg taagaaaata tagcaaattc cgatagtaag
    17881 cgtacagtcc ttatcgatca tgcatatatc ctggaatatg atcgcaagga tccaagatat
    17941 gaattgccgg tacatattcc agcccgggat gggcggcacc tatcatggct gtcccgcgca
    18001 cccgtcatgc tcgatgccct agccgccgcc gcgagactgc tcgtccagcc gtcacccgag
    18061 ctgatcgaga tcgtcgtcct cagcctgcga gtcagcctct ccgccgtggc cctggccacg
    18121 ctgctcggca tgccgctcgg cgccctgatc gcggtcagtc gctttccggg gcgacgccct
    18181 ctcatcgtcc tgctcaacgc cttcatgggg ctgccgccgg tggtggtcgg actcgtgttc
    18241 tacctgctgc tgtctcgcgc cgggccgctc ggctcgctcg ggctgctgtt cacgccggcc
    18301 gcgatggtga ttgcgcagac cgtcctgatc gtgccgatca tcgcggcgct ctcgcgccag
    18361 gtgatcgagg acgcgtggcg tgagtacgaa gagcaactgc gctcgctcgg agcccatcgg
    18421 ctcgacgcgg cgctcactct gctctgggac acccgctttt ccctcctcac caccgttctg
    18481 gccgggttcg ggcgggccgc cgcggaggtc ggagcggtaa tcatcgtcgg cggcaatatc
    18541 gatggcgtca cccgggtcat gaccacgacg atcgcgctcg agaccagcaa gggtgatctc
    18601 ccgcttgcgc tcgggctggg cgcgatcctg gtcggcatcg tgatcgtcgt gaacgcggcc
    18661 gcgtatctga cgaaggaagc ggcgcagcgc cgctacggat gagcagccgt cccattctgc
    18721 cgcttcgact gacgggggtg tcctttgccg cgggtggacg caacatcatc gatggagtgt
    18781 ccgtcgagct cggcgccggt ccggccacga tcatcctcgg ggcgaacggc gccgggaaga
    18841 gcgtcctcat gcgcatcatg cacggactgc tgcagacgac gtccgggcgc gtcgaatgga
    18901 gcgcaagcgc gctggcgtcg cgccggcaag ccatggtgtt tcagcgcccg gtgatgcttc
    18961 ggcgctcggc cctcgcgaac gtgctctatg ccctcgcttt ggcaggcgtc gagcggcggc
    19021 atcgcgccgc gatcgcgagg gatgccctgg aaagcgtcgg cctcgcggcc ctggccgatc
    19081 gtcccgcgcg ggtgctgtcc atcggagagc aacagcgtct cgctctggcc cgcgcgtggg
    19141 cattgcatcc ggaggtgctt tttctcgacg agcccaccgc cagcctcgat cccacgtcga
    19201 cccgtgaaat cgagaccgtc atccgcgcct tcgacgcggc cgggaccaag atcgtcatgg
    19261 cgacgcacaa cctcggccag gctcggcgcc tcggcgacga gatcctgttc ctcgaccgcg
    19321 gccgcctcgt cgagcgctca tcggtcgaaa ccttcttcgc gcgcccgcag accgcgcaag
    19381 ccgaagcttt tatcagagga gagctgcctt ggacctgatc cgccgaatcg tcatcgtcgc
    19441 gttgctcgcg ctggttggtg cgggcgcgct ccccgccttc gcgcagcaga agttcatcac
    19501 cgtcgcgtcg acgacgtcga ccgagcagtc ggggctgttc aagtacctgc tgccgatctt
    19561 cgagaaaaag agcggagtcc aggtgcacgt cgtcgcggtc ggaaccgggc aggcgctcga
    19621 catcggccgg cggggcgacg ccgacgtcgt gttcgtgcac gcgcggtcgg ccgaggagaa
    19681 atttctctcc gagggccagg gcgtgaagcg cttcccggtc atgtacaacg atttcgtgct
    19741 ggtcggtccg aaggcggatc cagcgaaagt cgccggcggc ggggacatcg tcgcggcgct
    19801 gcggaagatc aaggccgcgc aggcgccgtt cgtgtcgcgc ggcgaccgca gcggcacgca
    19861 catcgccgag ctcgcgctgt ggaaggacgc cgacatcgac atcgccaggg acaagggccc
    19921 ctggtacaag gacacggggc agggcatggg tcccgcgctc aacaccgcgg cgtcgatcgg
    19981 cgcctacatt ctctcggatc gcggcacctg gatctcgttc aagaaccgcg gcgatctcaa
    20041 gatcgtcgtc cagggcgaca aacgcctgtt caaccagtac ggcgtgatgc tcgtgagccc
    20101 gcagaagcat ccgaacgtga agaaggacct cgggcaggag ttcatcgact ggctgatctc
    20161 gcccgagggc cagaaagtca ttgcgggata caggatcgac gggcagcagc tgttcttccc
    20221 gaacgccgac cagcccggcg cctgaaaaca gcgctatatc cctgcgggaa cgacgcccct
    20281 gataaaatgg ggcgatgaag atcttcggca tcaccggctg gtccgggagc ggcaagacca
    20341 ctctgatcga gcagatcgtg ccgcgcttcg tgagcaaggg cctggtggtc tcgctgatca
    20401 agcacgcgca ccacagcttc gacgtggacc atccgggcaa ggactcgtac cggcatcggc
    20461 aggccggctg caccgaggtc ctcgtcagct cgccgaaccg ctgggtggtc atgcacgagc
    20521 tgcgcggcgc gcccgagccg acactggacg agcaactcgg gcgcatctcg ccttgcgacc
    20581 tgctgctcat cgagggatac aagcgctacc cgatgccgaa gctcgaggta tggcggagcg
    20641 agaacgccaa acccttcctc catcccgagg acaccaacat cgtcgccatt gccaccgacg
    20701 tcccgcccga ggcgggcccg agacgcttcg acctgaacga ccacgatggc atcgccgatt
    20761 tcgtgctcga gttcaacggg ctgaaatgaa caaggggctc ctcagcgtcg acgaagcgct
    20821 cgagcgcctg ctctcgggcg cgaataccgt cgcgggacgc gagaccgttc ccacgctcga
    20881 agcagtcggc cgcgtgctcg ccgagacgca acgctcgacc atgaacgtgc cgccgatgga
    20941 caacagcgcc atggatggct acgccgtgcg ccgcgccgac gtgcgcgcgc ccggcgcgcg
    21001 actcaaggtg tcgcagcgca tcgccgccgg cggcgtcggc gcggccctcg cgcccggcac
    21061 cgccgctcgg atcttcaccg gtgctcccgt ccccgagggc gcggacgcga tcgtcatcca
    21121 ggaggacacc gccgccgaag gcgacgcggt gatcgtgaac aaggtgcccg agccgggcga
    21181 atggatccgc cgcgtggcga gcgacatccg cgcgggcgac gcgatcctcg ccgccgggat
    21241 ccgcctgagg ccgcaggata ccgggctcgc ggcgtcggtc ggcatcgcga cgctgccggt
    21301 ggtcgggcgc gtgcgcctcg gcctattctt caccggcgac gagctcgtca tgccgggcga
    21361 cccactgccg cccggacgca tctacaactc caaccgcttc acgctgaacg gcctcgcgcg
    21421 cgccttcgac tgcgacgtgc gcgattatgg catcgtgccc gacagcctgg atgcgacgcg
    21481 cgcggtgctg cggcgcgccg cgtccgagtg cgacgtcatc gtcacgagcg gcggcgtgtc
    21541 ggtcggcgaa gccgatttcg tgaagcccgc cgtcgaggcc gagggcgagc tgctcatgtg
    21601 gcgcatcgcg atgaagccgg gccgcccgct cgcgttcggg cgcgtggcga aggccgcgtt
    21661 catcggcctg cccgggaacc cggtgtcgag cttcgtgact ttcctgctgt tcgtgcgacc
    21721 gttcctgctg aagacgcagg gtatcgcgca cgtcgcgccg cgcgcgatcg aggcgcgagc
    21781 cgacttcgac tggccgcgcc ccgatccccg gcgggagttc ctgcgcgtaa aatggaacgc
    21841 gcgcggcggc ttggatctgt acccgacgca ggattccgcc gtgctgacct cgacggcatg
    21901 ggccgacgga ctggtcgaca acccggcggg acaggcgatc cgcgcgggcg acggcgtgcg
    21961 tttccttgcg tactcggagc tgtatcggtg aagatcaagg tgctctattt cgcggggctg
    22021 cgcgagcagg tcggcagccg aggcgaggac ctcgacgtcc cgcccggcgt gaccaccgtc
    22081 gccggcgtgc gcgccctgct gcagtcgcgc ggcggtccct ggcagagcgc gctcgcgcaa
    22141 agcagggccg tgcgcatcgc ggtgaaccag gagatggccg cggccacaac gctggtgcgg
    22201 gccggggacg agctcgcctt cttcccgccg gtgacggggg gctagcggtg acggtgcgcg
    22261 tccagacgga agacttcgac gcgggcgccg agatcgcggc gcttcgccgg gggaacccga
    22321 agatcggtgc ggtggcgagc ttcatcggcg tcgcgcgcga cgtgaacgac ggcgacagcg
    22381 tctcgacgct cacactggag cactacccgg ggatgacgga gaaggcgctc gaaggcatcg
    22441 tcgccgaggc gcgcgcgcgc tgggacgtca tcgaactgct ggtcgtgcac cgggtgggtg
    22501 agatcaagcc gaccgagcag atcgtgctgg tcgtcgtcgc cggcgcgcat cgcggcgagg
    22561 cgttcgcggc gtgcgagttc gtcatggact acctgaagac gcgcgcaccg ttctggaaga
    22621 aggaagagac accgaagggc gcgcgctggg tcgatgcgcg cgagtccgac gacgaggccg
    22681 cggagcgctg ggctcccggc aagtcgagcg cggcttagga acgacgcccc ggatgtcggc
    22741 ctacggcttc gcggcagtcg gcatcggcgc cgcgctcggg gcctggctgc gatgggtgct
    22801 cggcatcgcg ctcaacctgc tcgtgccgac gctgccgctc ggcacgctcg cggcaaacct
    22861 gatcggaggc tacctgatcg gcgtggcggt cgcggtgttc gagcagaacg ccgtgctcgc
    22921 gccggagtgg cgcctgctcg tgatcacggg attcctgggc ggtctcacca cctattcgac
    22981 attctccgcc gagtcggtgg cgttactgtc gacgggacgc tacggttggg ctgcgacgca
    23041 cacgctcgcg cacctcggcg ggtcgctggc gatgacggcg ctcgggatcg cgagcgtgcg
    23101 gctgctccag aattgaggga tcgaagtccg aacccgcgcc gggccttcgc cggaggcgag
    23161 gaggaggagg atgccgccgg gctctgcgcc cggcgcggtc ggaactcggt ggctcagcag
    23221 tagccgagct ggttcttgcg ctcgacgtcg cgaaggcggc gctcgaggtc ggcgtggtcg
    23281 gtcgattgcg cgaggtacgc ctcgacgtcg cgacgcgggc cgggccgcaa gctcgcttcg
    23341 agccactgcc cgaatcgggc gaccagcgcc ttgagggtct cgccgcgcag cgcccgggcg
    23401 cgcgcttcga tggcgcgggt gtcgacgccc gaaggcttct cgttccgggc ggcggtgcga
    23461 aggtagccgt ctccttcgta aagcggatag gtggttctca tgatgcttcg tcctttctgc
    23521 gattggtgca tcgcacacaa gacaatatag gaagcggaac cattgttttc aaatcgtaaa
    23581 ttcagatctt cagcatcgtg attggtgatg tacgctaagt ccttgttgca tagcaacaag
    23641 gagcctaaaa tacgctgaat ggagctatat caactacgaa cattcgccgc cgtcgcggac
    23701 acgggccaca tcacccgcgc cgcggagaag ctgcacctca gccagcccgc ggtgagcgcg
    23761 catatcaagg gcctcgagga ggagctcggg gtgcgcctgt tcgagcgcaa cccaggcggc
    23821 atgctgctga cccgtgccgg ccgcgacttg cgcgggcagg ccgagaaggt gctcgccgag
    23881 gtcgagcgcc tgcggcagct cgcggcgacg ctgaagggcg atatcgtcgg tcggctgcgc
    23941 gtcggcacgg cgagcgatcc cgagttcgtg cgcgttggcg agttccttca ccgcacggtc
    24001 gagcgttacc cgctgctgga gctcgagctg catcaggaag tctccggcgc ggccctccag
    24061 caggtgcgca ccggcgacct ggacgcgagc ttctatttcg gcgacatcac gtcgcccgac
    24121 gtggccgggc tgcgcctgca cgacatggcg tactgcgtcg tcgccccggc cgcctggggc
    24181 gatcgcgtgc ggaacgccga ctggagcgat atcgcgcgcc tgccgtggat cgtcacgccc
    24241 tcgatcagca cgcacaacag gctggtgcgc gcgctgttca gcaggcacgg ggtcgagccc
    24301 gcgagggtgg tcgaggccga ccaggagccc gtcatcgcga gcctggtcgt ctccggcgtg
    24361 ggcgtctcgc tgatgcgcga gccgctcgcg ctgcagaagc aacggtccgg agagatctgc
    24421 atctgggaca aggcgcggct gcagaccgcg ctctggttca tctaccaggc cgaccgaagc
    24481 cacgatcccc tgatcgaggc gctgctgggg atcctcgggg agatctggca gatcgcgccg
    24541 gcgcccagtt tctagcgcga cggcgcctcg acgcggcccg cgaggcgcag gttcgcctgc
    24601 gtcaggcggt cgatcagcac gcgcatgaac gccttgttga agccgacctg gcaggcgtcc
    24661 gacgcggcct tgagcgcatc cgccttgatt tcgatcgccg tgaccgcggt cgtcgcggtg
    24721 atgctggtcg agcgccggcc ggtctggtcg ctgaaataga ggatctcgcc gaagcagccg
    24781 ccgcgcttga tcgtgttgag cggcttgccg aggagcgtga cggccacctc gccctcgatc
    24841 agcaggtaga agctgtcgcc ctggtcggcc tcgcggatga tcaccgtgcc ggcgtcgaac
    24901 gatttccagc tgccgatgcg cacgacctcc cagagctcga cgtcgtcgaa atcctggaag
    24961 aacgggaggc tgcggagctc gttgtacttc tccgagtcgg acaccgattc gccggcgagc
    25021 cgcaggctgg tgaacgcctt ggacaggtcc ttgccgaact ccatccagtt cgaatagcgc
    25081 tccttgcggt ccttgctgat cgcgcgattg acgatgcggt cgagcagcgg cggcaggtcg
    25141 ggccgcagcg tgagcaccgg cacggcgggt gtattgagga tcgcgtaggt caggcccgcc
    25201 gtggtcgtcg cgccctcgta aggcagccgc ccggtgagca ggcgatacat gaccacgccg
    25261 agcgagtaga tgtcggtctg gtggttcacg tcctccatgc ggatctgctc gggggacatg
    25321 taggccgggg agccgatgcc ggtgatctgc gtcgtctcct ggcccaggcg ctgctggaac
    25381 gacgcgccga agtcgctgac cttggcgtcg cccgagtcgg aaagcaggat gttgccgggc
    25441 ttgatgtcgc ggtggatgac cccgtgctgg aacgcgtagt cgagcgcgcg gatgcacttg
    25501 aagacgatct cgaccacctt cccgagcggc agcaggctgg cgacgtcggc gtgcgcctcg
    25561 agggtctcgc ccttgacgta ctccatgacg aggtagctgt actcgggctc gctcgccgcg
    25621 tccaggatct cgacgatgtg cggatgcacg agcttgccgg cgagcgcggc ttcggcgagg
    25681 aaggccttgc gcatcatgcg cgcggtctgc tcgttgatgt tctcgtcgaa ctggaatacc
    25741 ttgacagcga cgtcgcggtt ggcgaagggg tcgcgcgcga gatagacgcg gctcgtcgcg
    25801 ccgctgccga tctggcgcgt gatcgggtac ttgccgatgt gctcttcgag ctggaacttg
    25861 atggccaagc ggcccctccc agggcttaga tacggtttgc gctgcagtgt acaagcacga
    25921 agcgggccct gtcgcgggat tcgttccgga aaaccagtcc attcaaggcg cggccgcgcg
    25981 cgcggctcca gctccgcggc gggcgtcccg gcttgaaatt cgctgatccc gccccatttc
    26041 ccctacagct catccggata tccctatgcg attcgacaag ctgacgacga aattccagca
    26101 ggccatctcc gatgcccaga gcatcgcggt cggccaggac aacccgttca tcgagccggt
    26161 ccacctgctg cagggcctgc tgcagcagga ggacggcacc acctcgtcgc tgctctcgcg
    26221 cgccggggtc aacgtcgccc gcctgcgcga atcggtgaag caggcgctcg cgcgcctgcc
    26281 gaaggtcgaa ggtcagggcg gcgaagcgcc gatctcccgc gatctcaaca acctgctgaa
    26341 tgtcgccgac cgcgaggcgc agaagcgcaa ggaccagttc atcgcgtccg agatgttcct
    26401 gctggctctc gccgacgaca aaggcgaggc cgggcgcctg ctgaaggaga acggcggcaa
    26461 tcgcaaggcg atcgagcagg cgatcgaggc ggtccgcggt ggcgagtccg tcggcagcca
    26521 ggaagccgag gggcagcgcg aagcgctgaa gaagtacacg ctcgacctca ccgagcgcgc
    26581 acgctcgggc aagctcgacc cggtgatcgg ccgcgacgac gagatccggc gcgtgatcca
    26641 gatcctgcag cggcgcacca agaacaaccc cgtgctcatc ggcgagccgg gcgtcggcaa
    26701 gaccgcgatc gtcgagggcc tcgcgcagcg catcgtcaac ggcgaggtcc ccgagaccct
    26761 gaagaacaag cgcgtgctgt cgctcgacat ggcggcgctg ctcgcgggcg ccaagtaccg
    26821 cggcgagttc gaggagcggc tgaagtcggt gctgaaggag ctcgcgcagg acgaggggca
    26881 gaccatcgtt ttcatcgacg agctgcacac catggtcggc gccggcaagg ccgagggcgc
    26941 gatcgatgcc ggcaacatgc tcaagcccgc gctcgcgagg ggcgagctgc attgcgtcgg
    27001 tgccacaacg ctcgacgaat accgcaagta catcgagaag gacgccgcgc tcgagcgcag
    27061 gttccagaaa gtcctcgtgg aggagccgtc cgtcgaagcg acgatcgcga tcctgcgcgg
    27121 cctccaggag aagtacgagg tccaccacgg ggtggacatc accgacccgg cgatcgtcgc
    27181 cgcggcggag ctctcgcacc gctacatcac cgaccgcttc ctccccgaca aggcgatcga
    27241 cctcatcgat gaggcggccg cgcgcatcaa gatggagatc gactccaagc ccgagagcat
    27301 ggaccggctc gaccggcgcc tgatccagct caagatcgag cgcgaggcgg tgcggaagga
    27361 gaaggacgag gcctcccagc gccggctcgc ggccatcgag gacgagatca agcgcctcga
    27421 gcgcgagtat gccgacctgg aagaggtgtg gaaggcggaa aaggcccagg tgcagggttc
    27481 ccagcacgtg aaggaagagc tcgagaagct caagctcgag atggaggcgg ccaagcgcaa
    27541 gggcgactgg aaccgcatgt cggagctgca gtacggcaag atcccgcagc tcgaggcgca
    27601 gctgaagaag gaagagaagg gctccgccgc cgcgaagccg aagctcgtgc gcacgcaggt
    27661 cggcgccgag gagatcgccg aggtggtgtc gcgcgcgacc ggcatcccgg tctcgaagat
    27721 gatgcagggc gagcgcgaca agctgctgaa aatggaagac aggctgcacg agcgcgtcgt
    27781 cggccaggac gaggcagtgc gcgtcgtcgc cgacgccatc cggcgctcgc gcgcgggact
    27841 ctcggacccg aacaagccct acggctcgtt cctgttcctg gggcccaccg gggtcgggaa
    27901 gacggaattg tgcaaggccc tggccggctt tctcttcgat tccgaggacc acctcatccg
    27961 tatcgacatg tccgagttca tggagaagca ctcggtcgcg cgcctgatcg gggcgccgcc
    28021 ggggtacgtc ggctacgagg aaggcggcta tctcaccgag gccgtgcgcc gcaagcccta
    28081 cagcgtgatc ctgctcgacg agatcgagaa agcgcacccc gacgtgttca acgtgctgct
    28141 gcaggtgctc gacgacggcc gcatgaccga cggccagggg cgcaccgtcg acttcaagaa
    28201 caccgtgctc gtgatgacgt cgaacctcgg ctcgcacatg atccagcaga tggcgggcga
    28261 cgactacggc gtgatcaagc tcgcggtcat ggccgaggtg aagacgcagt tccggcccga
    28321 gttcgtgaac cgcatcgacg agatcgtcgt gtttcacgcg ctcgacgaga agcacatcaa
    28381 ggacatcgcg aagattcagc tgcgttatct cgagcagcgc ctggcgaagc ttgagatccg
    28441 gctcgacctg tcggactccg cgctcgccga gctggggaag acgggcttcg accccgtcta
    28501 cggcgcgcgg ccgctcaagc gcgcgatcca gcaggagatc gagaacccgc tcgcgaagca
    28561 gatcctcgag ggcaagttcg gcccgaagga caccgtgcgc gtcgaatacc gcggcaaccg
    28621 gatgcagttc gagaaggcgg gcgccaaggc cgccgagaag gccccggcgc accactgagc
    28681 gccgggccgc ggcgtcactc gaccttgatg ccgatgtcct tcacgagctt gccgtacttc
    28741 tcgtaatcgc gccggatcat ctgcgcgaac tggtcgggcg tgagcgggtt cgcctgcatg
    28801 ccgcccgcct tcgcgaggcg gccgtcgacg tcgcggtcgg tcagcgcctt gttgacctcg
    28861 tcgtgcagct ttgcgatgac cggcttcggc gtccccgagg ccgtgaacag gcccagccag
    28921 atcaccacct cgtagccggg gtagaactcg ccgatggtcg gcagctccgg gaaaaggctc
    28981 gagcgcttcg gcccggtcac cgcgagcgcg cgcagcttcc cggcacggat ctggcccgcg
    29041 ctcgaggtgc cggagaacat cgcggcggtc tcgcccgcga cggtcgccgt cgtggcaggc
    29101 gagccgcctt tgaacgggac atgcacgagg ttgatgccgg cgcgctgctt cagcatctcc
    29161 atcgccagct cgtgctggct gccgttgcct cccgaggcgt acagcaacgg gggattcgcc
    29221 ttcttcgcgt actcgatgaa ttcctggaag ctcttaaccg gcagcgaagg attcaccgag
    29281 agaacccact ggttcagcac gaccgatgcg accggcgcga ggtccttcat cgtgtcgagc
    29341 ggcatgttct tgtagatgtg cggattgacc gtcacgttgc tgtcggcgaa gaggccgagc
    29401 gtatagccgt cgggctcggc cttcgccacc tggtccatcg ccacgttgcc gttcgcggcc
    29461 ggccggttct cgacgatgac cggctgcttc atctgctcgg agagcttctg gccgacgacg
    29521 cgcgccgaga tgtccggcgc gccgccgggc ggaatcggga cgatgatcct gatcgggcgc
    29581 gagggatacg cttgcgccga cgcgtccgtc gcggcgcaga agagcccggc gacgagcgcc
    29641 aggaccgcaa tcagcgattt catgatgtca ctctccttgt cgtcgttttc gcggtgcccg
    29701 cctgcgcgcg cacggcgcgt gcgcggacga gcagctgttg ccagagcttc gacgtgtcgg
    29761 cccgcagcgc gttctcggcg tcggcgtcga gcacgaacca atcgccgcgc gcgatctcgg
    29821 catccagctg tcccggcgcc cagcccgagt agcccgagaa gaagcggatc gtggcgggcg
    29881 gctcgtgcag cagccggtcg atcgcgtccg ggtcgaccgc gaggtgcagg cccggggcca
    29941 tggtgatcac tccggccggc gccgactccg cggcgaacag cgcgagcagc gcgttgggcg
    30001 ccaccgggcc gccgaagcgc atcggctggt cgaagcggcg gaaccgctcg ccgggaagga
    30061 cctccgccag gctgaggctc agcggccggt tgacgatcac tccgatcggc gcgtccgcgc
    30121 cgcgcgcgac gaggaccacg ctttcgctga agttgggatc gggcagcccg ggcctcgcga
    30181 cgaggaacac gccgcgggcg gattcctggg cggggacggc cgggagcagc gccgcgagca
    30241 gcagcgccgc gcacgctccc ttcaggagcg ccgggcggct cacgagcatc acgcgggcac
    30301 ggagcgcctc agccgagcgc gggcgcggag cgcaccgacg cggtgatcgc gcgcgcccgg
    30361 cgaagcagct cctcccacag cgaatcgccg ctgtccttgc ggaacaccat ctcctcgtcg
    30421 gcgtcgagca cgtgccacag gccgcggtcg atctcctggc gcagctcgcc cggccgccac
    30481 gcgacgtagc ccacgaagta gcgcgcgtcg ttcggcgtct tctcgatgat gtcgtcgacg
    30541 accgttccct gcgtcgcgag atagaggtgc ttcatcatct ggatcgagcc gctgccggga
    30601 ttgtggtcgg tgcgcaccat ggcgaagatc gcgcgacgca gcatcggccc gccgagatag
    30661 accggatcgt gcacctgctt ggaaggctcg tgctcgggaa acaggctcgc gagcgagcgc
    30721 tgcgtcggcc ggttgatgat gatgccgacg tgccggttgc cttcgaccgg caccgcgatc
    30781 agcaccgtgt gccggtagtc gggatcgctg agcgccggcg acgcgaccag gatgacggcc
    30841 ccggtgtctt cggtcttctg agccgctccc ggggcggcca tccccatgac caggagcagg
    30901 gcaaaggcgg cggcgatcca gcgcatcggg tcctccggcg gcatcacttt ggggcaagtg
    30961 tagcggaaga accgcgtttc cgggacccgg acgcccgggc cgcgcgccag caatcgcgca
    31021 ggtgatcgtc caccatgccg accgcctgca tgaacgcgta gcagatcgtc gatccgacga
    31081 aggtgaagcc gcgccggcgc agatcgccgc tcagggcgtc ggattcgcgc gtgcgcgccg
    31141 ggacgtccgc catccgccgc ggccgccgct tcatcggccg gccgccgacg aagcgccagg
    31201 cataggcgtc gaaggagccg aaggccttct ggacttcaag aaacgcgcgg gcgttgcgca
    31261 ccgcgccctg caccttcagg cggttgcgca cgatcccggg atcggcgagc agcgcgcgga
    31321 ccctgcgcgc gtcgaaggcg gcgacgcgtc gcggatcgaa gttcgcgaac gcgcgccgat
    31381 aattcgcgcg cttcgcgagg atagtcgacc acgacaggcc ggcctgcgcg ccttcgagga
    31441 tcaggaactc gaacagcgtg cggtcatcgt ggaccgggac gccccattcg cggtcgtggt
    31501 actcgacata ggcctcgctg ccgaggcacc acgggcagcg gcggcgttcg gagcgggggg
    31561 ccatcggccg atgctagcgc atcggccgcg ccgcttcagc ggacgccgag cgacgtgtgg
    31621 ttcccggcct gcgggggcac gaaagcgggc gcgcaccggc ccgacgcggt cgacgcgacg
    31681 agcacctcgt tcagcttgtc gaagtcgagc ggcttcacca ggtggtgatc gaagccggcg
    31741 gcccgcgact gctcccggtc gacctggcgg ccgtagcccg tcatcgcgat caggacgacg
    31801 tcgcgcatcg ccggagtctc gcgcatccgc tgcgccacct cgtgcccgct catgccgggc
    31861 atgccgatgt ccatgagaac gacgtcgggc cgatactcgg ccgcggtgcg cagcgcgtcc
    31921 ggtccgttgt gcgccttgcg cacgttgtgt cccgacatct cgagaatgat cgccatgctc
    31981 tcggccgaat cgacgctgtc gtccaccacg agcaccttga gctgccggtt ctcgcgctcg
    32041 ctcttcggaa cggcgggcct cgcctgcggc tcgggcgtct ccacggcgcc gttggcgcgc
    32101 ggcaggcgga tgacgaactc gctgcccttg ccgggcccgt cgctgaacgc ctcgatgcgg
    32161 ccgccgtgca tctcgaccag gccgcgcgcg agcgcgagcc cgatgccgag gccgccttcg
    32221 ctgcgcgcga tggaatcgtc ggcgcgtcgg aacagctgga aaaggttcgg cagcatctcg
    32281 gccgcgatgc cgacgccggt gtcgcgcacc cggatgagcg cctctcccgg cattcccgcg
    32341 agggtctcca gcgcgaccgt gatcctgccg ccttcctgcg tatatttcgc ggcgttggtg
    32401 aggacgttgt cgacgacctg cgtcagccgc acgcggtcgc cgagcacggg gatcgcctcc
    32461 tcgggcacgt cgacctggag cgcatggccg cgctggtgga tgagcgtgcg cgtggtctcg
    32521 accgccgcgg cgacgaccga cttcagctcg atcggctcgc gccgcagcgt caccttgccg
    32581 cgcgtgatcc gcgagacgtc gagcaggtcg tcgatcagcc gcgtgaggtg gccggtctgg
    32641 cggccgagga cgtcgcgcgc ccaccgcagc tgcgggtcga tcgcggggct ggccttcatc
    32701 acgtgcgcga cgttgctgat ggccgccagc ggattgcgca gctcgtgcga gagcatcgcc
    32761 aggaactcgt ccttgcgccg gtcggcctcc tgcagctcct gctcgaagcg cttgcgctcg
    32821 gcgatctcgc gctcgagcac ttcgttggtc ttggcgagct cggcgttggc ctgctccagc
    32881 tcgcgcgtct tctccgcctg cagcgtggtg ttggcgagcg cgagcgcctc gttggcgcgc
    32941 tccagctcgc ggcgcttgcg gtagagctcg accagcacct gcaccttgcc gcgcagcacc
    33001 tcggggacga tcggcacgta gcagtagtcg accgcgccca gctcgtagcc cttcagctgg
    33061 tcgaggttgg tgacgtgcac agcggtcacg aagatgattg gcgtcttctc gaagcgcggg
    33121 tgctggtgga tcagcgccgc ggtgtcgaag ccgttcatcg tcggcatgtt gacgtcgagg
    33181 aggatggcgg cgaagtcggc ctccatcagc ttctgcaacg cctcggtccc cgagcgggcc
    33241 ttgaccaggt tctcgcccag ggttccgaga atggcctcgt acgacagcag ccgctgcggc
    33301 tggtcgtcga cgaggagaat gttgaccttg tcgtcgttgc ggttcatcgc tctcccattg
    33361 ccttcgcttc gcccgcgcgc cgtcacgggt gcagccacat gcgcagcatc gacagcagct
    33421 cgccgctgtt gacgggcttc gcgatgtaat ccgacgcgcc ggcctgcagg cacttctcgc
    33481 ggtcgccttt catggccttc gcagtgagcg cgacgatcgg cagccgcctg aactgcggca
    33541 cctcgcggat gcgccgtatc gtctcgtagc cgtccatctc cggcatcatg atgtccatca
    33601 gcacgagcga caggtccggc gtggtctcca tgagctggat cgcctcgtgg ccgttggtcg
    33661 cgatggtggc ctgcatgccg tgccgctcga gcaggctcga aagggcgaag atgttgcgca
    33721 cgtcgtcgtc gaccacgagc gccttcttgc cgctgagcgc gtcgtcggtc tcgtgcagcg
    33781 tctcgagcat cttgcgcttg ccgtcgggca ggttcgcgac cacgcggtgc aggaacaacg
    33841 ccgtctcgtc gagcaggcgc tcgggcgagc gcacgtcctt gaggacgatg ctcttcgcca
    33901 tcttgcgcag gcgggcctcg tcgtccgggg tgagatcccg acccgtgaag acgacgatcg
    33961 ggacgtcgcg caggcgctcc tcgcgctgga tctgctcgag caggtcgaag cccgagatgt
    34021 cgggcagcct cagatcgagg accacgcagt cgaacggctt gctgcgcagc gtctcgagcg
    34081 cctccttgcc cgtgccgacg caggtgatgt cgacgtcgtc gtgcgcgatc aactcggcca
    34141 cgctcttctg ctcggccgga ttgtcctcga ccacgagcag cttccgcacg cgctcggacg
    34201 cgaacgcctt gatgcggtcg aacgccgtct cgaggttgtc cgacgtgagc ggcttgttga
    34261 ggtaggcgaa cgcgcctcgc tcgagtccgt gctggcgctc ctcctcgacg gtgatcacct
    34321 gcaccgggat gtggcgcgtc gtgtggtcgt gcttgagctg cgacagcagc gtccagccca
    34381 gcatgtccgg caggaagatg tcgagcgtga tcgccgtcgg ccggtactgg cgcgcgagcg
    34441 acagcgcttc gccgccgcgc atcgcgacca gccccttgaa gccgcggccg cgcgccaggt
    34501 cgagcagaac gcgcgcgtag tgcgggtcgt cctcgacgat gaggatggag gggtcgccct
    34561 cctgcagggt ctcgcggtcg tcggggacgg tctcggtgcg cggggccggc agcacgatcg
    34621 gctggaacgc caccggcgct tccgcgatgc ggtgcgccgc gggttgcggc gccgccgcgc
    34681 cctggtagtg cagcggcagg tagagcatga aggtgctccc cgcgcccggg atggaatgca
    34741 gccggatctc gccgccgagc aggtgcgcga gctcgcgcga gatcgcgagg cccaggcccg
    34801 tgccgccgta ctttctcgcc gttccggcgt cggcctgctg gaacgcctcg aagatgatct
    34861 tctgcttctc cggcgggatg ccgatgcccg tgtcgctcac cgagaagcgc accaccttgt
    34921 cggcctggcc gagcaccggg tgctcggggc tccagccgct ctcgacgatc gacacgtcga
    34981 tgcgcacgcc gccctgctcg gtgaacttga acgcgttcga gagcaggttc ttgagcacct
    35041 gcagaaggcg cttcgggtcg gtcgagatcg tcctgccgag gtcggagtcg aagttcgccg
    35101 agaacgcgag cttgcgcgac tcggcgatgt gacggaagtt gcgctcgacc gtgtcgcgca
    35161 gcgtcgcgaa cggcagctcc tcgctctcga ccgtgaccgt gccggactcg atcttggaca
    35221 ggtcgaggat gtcgctgatc aggttgagca ggtcggtgcc cgcgccgtgg atcgtcttcg
    35281 cgaactcgat ctgcttcgcc gacaggttgc cctcgacgtt ctcggcgagc tgctggccga
    35341 ggatcaggat ggagttcagc ggcgtgcgca gctcgtgcga catgttcgcc aggaactcgg
    35401 acttgtagcg cgaggtgagg gcgagctcgg cggccttctc ctcgaccgcc cggcgggcct
    35461 ggtcgatctc gcggttcttg cgctcgacct cctcgtactg cgcggccagc tcgcgcgcct
    35521 tcttcgcgag ctcgtcgttg gtctgctgca gctccttctg ctgcgcctgc agctcggcgg
    35581 cgagctgctg cgactgctgc agcaggcctt cggtgcgcat cgtcgcctcg atggtgttca
    35641 gcacgatgcc gatcgagccg gtcagctgct cgaggaacgc gaggtgcgac ggcgtgaagt
    35701 gcgtgagcgc ggcgagctcg atcacggcct tcacctggcc ttcgaagagc acaggcagca
    35761 cgatcacgtt cgcgggcctc gcggtcacga gtcccgcgtg cacgaggatc gactcgggcg
    35821 cgatgtcggc gagcaggatg cgctggcgct cgcgcgcgca ctgcccgacc aggccgtcgc
    35881 cgatgcggta gcgccgcggc gcgcccggat cgacgtggtc ggcgtagccg gcgagctgct
    35941 tcagcaccgg cgggaactcg ctgtcgtcca tcacgtacat gacgccctgc tgcgcgttca
    36001 cgagcggcgc aagctccgag agcagcatct gcgccaccgt cagcaggtcg cgctgtccct
    36061 gcatcatcga gccgaagcgc gcgaggttgg tcttgagcca gtcctgctcg gtgttgcgct
    36121 cggtggtctc gcgcaggttg ccgatcatgg cgttgatgta gttcttcagc tccgcgacct
    36181 cgccctcggc ctcgacctgg atcgaccggg tcaggtcgcc cttggtcacg gcggtcgcca
    36241 cttccgcgat cgcgcgcacc tgggtcgtga ggttggcggc gagcaggttc acgttcgccg
    36301 tcaggtcctt ccacgtgccg gccgcgcccg ggacgttggc ctggccgccg aggcggcctt
    36361 cgacgcccac ctcgcgcgcc acggtcgtca cctggtcggc gaaggtcgcg agcgtgccgg
    36421 tcatcgagtt gatggtgtcg gcgagcgccg ccacctcgcc cttcgactcg acggtcagct
    36481 tctgcgccag gtcgccgttc gcgaccgcgg tcacgacctt gacgatgccg cgcacctggt
    36541 tcgtgaggtt cgcggccatg acgttcacgt tgtcggtcag gtccttccac gtgccggcca
    36601 cgcccttcac ctgcgcctgg ccgccgagct tgccctcggt gccgacctcg cgcgcgacgc
    36661 gcgtcacctc ggcggcgaac gcgttcagct ggtcgaccaa cgtgttcagc gtgttcttca
    36721 gctcgaagat ctcgccgcgc gcgtcgaccg tgatcttgcg cgagaggtcg ccgcgcgcga
    36781 tggccgtcgc gacgtcggcg atgttccgca cctggccggt gaggttggag gccatccagt
    36841 tgacgttgtc ggtgaggtcc ttccacgtgc ccgcgacgcc gggcacctgc gcctggccgc
    36901 cgagcttgcc ttccgtgccg acctcgcgcg cgacccgcgt cacctcggcg gcgaaggagt
    36961 tgagctggtc gaccatcgtg ttgatggtct ccttgagctg aaggatctcg ccgcgcacgt
    37021 ccaccgtgat cttgcgcgag aggtcgccga gcgcgatcgc ggtcgcgacg tcggcgatgt
    37081 tgcgcacctg cgccgtgagg ttcgacgcca tcgagttcac gttgtcggtg aggtctttcc
    37141 acgtgccgcc gacgcccgaa acctgcgcct ggccgccgag cttgccctcg gtgccgactt
    37201 cgcgcgcgac gcgcgtcacc tcggcggcga aggagttgag ctggtcgacc atcgtgttca
    37261 gcgtgttctt gagctccagg atctcgccct tcacgtccac cgtgatcttg cgcgagaggt
    37321 cgccccgcgc gacggcggtc gccacctcgg cgatgttccg cacctggttg gtgaggttcg
    37381 acgccatgaa gttgacgttg tcggtcaggt ccttccacgt gcccgcgacg ccggacacct
    37441 gcgcctggcc gccgagcttg ccctcggtgc cgacctcgcg cgcgacgcgc gtgacctcgc
    37501 cggcgaacgc gttcagctgg tcgaccatgg tgttgatcgt ctgcttcagc tggaggactt
    37561 cgcccgagac gtcgacggtg atcttcttc
//
LOCUS       DATDMW010000040        18673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_757344, whole genome shotgun sequence.
ACCESSION   DATDMW010000040 DATDMW010000000
VERSION     DATDMW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18673
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757344"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VI319_01545"
     CDS             <1..410
                     /locus_tag="VI319_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY6862556.1"
                     /translation="ALFDMHLHHDFDPLNENEESKTPLQLLDQIRARVAVALPPRYNR
                     FPNNFSHIFAGSYAAGYYSYKWAEVLSADAYSMFEERRDSAGVLDPETGARFRDEILA
                     VGGSRPAIESFKAFRGRAPRIDALLRHNGMIAA"
     gene            485..1057
                     /locus_tag="VI319_01550"
     CDS             485..1057
                     /locus_tag="VI319_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY6862557.1"
                     /translation="MRIVLLAGIIVLAVSSAYGQMYRWVDKDGRTHYTQTPPPPDARS
                     VEKKSLDAGAGSAAPGAMPYGELPYATQNAARNYPVTLYTSPNCGAPCEQARAALVRR
                     AVPFKEVSVAGQQDIENLKAAGGTGLPHLVVGNLKQTGFSEDSYKSTLDTAGYPQSGP
                     RTSIEALRTMDAPAKTPAPGGGGSSQEAPR"
     gene            1119..2093
                     /locus_tag="VI319_01555"
     CDS             1119..2093
                     /locus_tag="VI319_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862558.1"
                     /translation="MRMETSFLRAACAAAAALASCAALAQAYPTKPVHFVVPFAAGGP
                     ADIYARFLGNRLEKTLGQPFVIEDRPGGGAIIGTEVVVKSAPDGYTILAMSNTHTVNE
                     SLFTKKPYKLMQDLTPIAPINYSDLLLVVNPSVPAKNLKELIALVKSKPGQLNYASSG
                     PGTPYHMAGELFKAMAGLDIVHVPHKGSDQARTSVMAGQTQMMLDAVTTMAQIARSGK
                     VRAIATTGKTRSSIMPEVPTMDEAGVPGYEATIWLGVMAPAGTPKPIIDKLNAEIRKT
                     VHQPDVKDAWAKQGAVAMDMTPQQFSKFLQQDIEKWAKVVKISGAKVD"
     gene            complement(2114..3730)
                     /gene="aceB"
                     /locus_tag="VI319_01560"
     CDS             complement(2114..3730)
                     /gene="aceB"
                     /locus_tag="VI319_01560"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY6862559.1"
                     /translation="MAKTATVKKSHSSQGIEILAPVTAGYEQVLTSAALEFLAALHRE
                     FEPQRQELLSRRAERQRGFDAGAMPDFLPETASIRTSEWSIAAQPADLLDRRVEITGP
                     TDRKMVINALNSGASTFMADFEDANCPTWANMVEGQINLRDAVRRTISFEQGGKHYRL
                     KDRTAVLIPRPRGWHLDEKHVHVDGRPVSGAIFDFALYFFHNAKELLARGTGPYFYLP
                     KMESHLEARLWNDIFKRAQSALGVPQGSIRATALVETVLAAFEMDEILWELKEHSAGL
                     NIGRWDYIFSCIKKFRSHGDFCLADRAQVTMTSPFMRAYALLLVKTCHRRGAPAMGGM
                     AAQIPIKDDPAANAAAMEKVRQDKLREVTDGCDGTWVAHPGLVPIAKEIFDQHMPQPN
                     QYGRQRPDVQASAADLLRFEPEKPITEAGLRNNISVGIQYLGAWLAGNGCVPVFHLME
                     DAATAEISRSQIWQWIRSDHGVLDDGRKVTKELFSALLREELSKVRETLGEAAWRAAR
                     YEDAADLFERITNDDRYVEFLTLPAYAMID"
     gene            complement(3889..5289)
                     /gene="ntrC"
                     /locus_tag="VI319_01565"
     CDS             complement(3889..5289)
                     /gene="ntrC"
                     /locus_tag="VI319_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY6862560.1"
                     /translation="MKPVWVIDDDRSIRWVFEKALTREGIPFKTFAQATEALDALSVG
                     APQVLVSDIRMPGASGLDLLQNVKQRYPNLPVIIMTAYSDLESAVAAFQGGAFEYLPK
                     PFDVDQAVELIRRALDESLREGEAEEAPGATPEILGQAASMQEVFRAIGRLSQSSATV
                     LITGESGTGKELVARALHRHSPRAGNPFVAINTAAMPKDLLESELFGHERGAFTGAQS
                     MRRGRFEQAEGGTLFLDEIGDMPAELQTRLLRVLSDGTFYRVGGHQSIRASVRVIAAT
                     NQEIEQRVQDGAFREDLYHRLNVIRLRLPSLRERRDDIPLLARHFLAKSARDLGAEPK
                     RLSDAAMKYLAAQDLPGNVRQLENVCHWLTVMAPTQIIEVSDLPPELRQDPAAPEPDW
                     LGALARETERRLARGDADIMSALLRTFEKTLIAKTLAHTGGRRMEAAALLGIGRNTIT
                     RKIAELGIEGEEPPER"
     gene            complement(5305..6360)
                     /gene="glnL"
                     /locus_tag="VI319_01570"
     CDS             complement(5305..6360)
                     /gene="glnL"
                     /locus_tag="VI319_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEY6862561.1"
                     /translation="MTTAAFQGLDLLATAVVWLDAGLVVRYANPAAEATFALAQRSML
                     GHRLDELFADAQALEAQLRQALRDERGFTDPDLVLAPIGQEPVHLNCLASPVESGGPG
                     VLLELRPIDQRLRIEREEQRLTQHMANRELIRNLAHEIKNPLGGLRGSAQLLERELDR
                     LELREYTQVIIKEADRLQTLMDRLLTPHRPPTHVPMNIHEVCERVRSLILAEFPHGIG
                     IRRDYDVSLPEFRGDPEQLIQAVLNIARNAAQAIAADPARHGGEIVLRTRVSRQVSLV
                     KRGHGLALELQVIDDGPGVPAEIRDRIFHPLVSGREGGTGLGLTLAQTFVQHHHGVIE
                     CESQPGSTCFRITLPLR"
     gene            complement(6410..6844)
                     /locus_tag="VI319_01575"
     CDS             complement(6410..6844)
                     /locus_tag="VI319_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY6862562.1"
                     /translation="MTIIASWCGWAQAQGEIYRCPDAMGRPTYTNVKRDTVGKGCTMV
                     TREVSVVPAIPVARPVPAKASGGAMLSRDESRRKILENELESEQRLLGDAKQKLAEQQ
                     GQRSGDERNYARVEDRLKPFQETVDLHQKNIEQLQHELGNYR"
     gene            complement(7000..8415)
                     /gene="glnA"
                     /locus_tag="VI319_01580"
     CDS             complement(7000..8415)
                     /gene="glnA"
                     /locus_tag="VI319_01580"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY6862563.1"
                     /translation="MAMSAADVMKLIKDKEVKFVDLRFTDTRGKEQHVSVPVKSFGMD
                     KFESGHFFDGSSIAGWKGIEASDMLLMPDPDSARMDPFTDEPVLNLTCDAVEPADGKG
                     YDRDPRTIAKRAEAYLKSSGLGDVAYFGPEPEFFIFDSVTWNVDMSGCFVKIKSEEAP
                     WSSGEEYEGGNMGHRAPVKGGYFPVPPADTLQDIRNAISLALEQQGVEVEVHHHEVAA
                     AGQCEIGTKFNSLVKRADWMQILKYTIWNVAHSYGKTATFMPKPIVGDNGSGMHVHQS
                     VWKGGQNLFSGNGYSGLSDFALYYIGGIIKHAKALNAITNPGTNSYKRLVPGFEAPIN
                     LAYSSRNRSAACRIPLVTNPKARRVEVRFPDPTSNAYLAFAAMLMAGLDGVQNKIHPG
                     DPVDKNLYDLPPAQAKKIQNVCSSLDMAIDHLNKDREFLTKGGVFSDDMLDAYCALKM
                     EEVTRFRMTTHPVEFDMYYSA"
     gene            8665..9120
                     /locus_tag="VI319_01585"
     CDS             8665..9120
                     /locus_tag="VI319_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6862564.1"
                     /translation="MDSPDDILARARERARSMKLPYAGALLPAEAHALVTKAADAKLI
                     DVRTKAELEWVGRVPGAVTIEWNTWPGGAQNPNFLRELQDQVKAKDAPVLFLCRSGGR
                     SHNAAAAATQAGYANAFNVLEGFEGDKDTQGHRNTVGGWRRAGLPWVQG"
     gene            9173..10417
                     /locus_tag="VI319_01590"
     CDS             9173..10417
                     /locus_tag="VI319_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY6862565.1"
                     /translation="MKQLSGSAVAALACAAWLFAAPATQAQEFRILQPLGDKPMKMKN
                     GDYYVPTTPDTAMWGSLPNTNTKPVLSVPSGSVVTIDTVSHEGVLEDQGKDPVKFFGQ
                     FGVKPAQVLKDAQAIAASSIPHDFVKDGPHVVTGPVEVQGAKVGDVLMVEMIGLQPRV
                     PYGVISNRHGKGALPGEFPENQGQQPGADTAHPELYANVFTFVPIRKINGTWYGIVKG
                     KSGPEAHIPLKPFNGTMGVAVNTQEKPNSVPPGAYAGNLDVNELSVGAKLYIPVQVDG
                     ALFYAADPHFVQADGEVALTAIEGSLRSTYRLTLLKAGDPRLPMKAPMKNPFAETKEY
                     WIPIGLNTDLNEAMKDATRQTVQFLSEKLGMDRATALAYASAGVDFQVTQVVDRVKGV
                     NAIIRKSDFASARGAGTKGKKK"
     gene            complement(10434..11261)
                     /locus_tag="VI319_01595"
     CDS             complement(10434..11261)
                     /locus_tag="VI319_01595"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY6862566.1"
                     /translation="MDVVLLVKALVMGVVEGLTEFLPISSTGHLILAGELLRFSDEKA
                     KVFEIVIQTGAMLAIVWEYRLKFWRVLAGLPSDRGAQRFAVNLLIAFLPAALLGLAFH
                     NAIKTHLFHAVPVALAFIVGGVIILWVERALRPRVRVDSVDAMRWSDALKVGVAQAFA
                     LIPGTSRSGATIIGGMLFGLSRRAATEFSFFLAVPTLVAAGVYDLWKNRGLLSAGDLG
                     VFAVGSVAAFVSAFLCVRWLLRYIATHDFTPFAWYRIAFGAVVLATAWGGVVNWSAG"
     gene            complement(11275..13146)
                     /gene="thiC"
                     /locus_tag="VI319_01600"
     CDS             complement(11275..13146)
                     /gene="thiC"
                     /locus_tag="VI319_01600"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY6862567.1"
                     /translation="MNANPKFLAKTAHVDEAAVKPLPSSRKVYVEGSRPDVRVPMREI
                     RQSDTPAQFGAEKNPPIYVYDTSGPYTDPAVAIDIRAGLAPLRGRWIAERGDTEELAG
                     PTSSYGRERLADPKLAELRFDLKRRPRRAKAGRCVTQMHYAKQGIVTPEMEYIAIREN
                     QRRDALSELITREHPGESFGASIQRLITPEFVRDEVARGRAIVPANINHPESEPMIIG
                     RNFLVKINANIGNSAVTSGIAEEVEKMTWAIRWGGDTVMDLSTGRHIHETREWIVRNS
                     PVPIGTVPIYQALEKVDGKAEELTWAIFRDTLIEQAEQGVDYFTIHAGVRLPFIPLTA
                     KRMTGIVSRGGSIMAKWCLAHHEESFLYTHFEEICEIMAAYDVSFSLGDGLRPGSIYD
                     ANDEAQIAELKTLGELTDVAWRHDCQVMIEGPGHVPMQLIKENMELQLEYCKEAPFYT
                     LGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDKEDVKDGIIAYKI
                     AAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDKAKQFHDETLPQEGAKL
                     AHFCSMCGPHFCSMKITQDVRDYAEKHGVSEHDALKRGMGEKAIEFVKKGAEIYHKT"
     regulatory      complement(13185..13290)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13398..14063
                     /locus_tag="VI319_01605"
     CDS             13398..14063
                     /locus_tag="VI319_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY6862568.1"
                     /translation="MNVEQARFNMIEQQIRTWEVLDQDVLDLLYAVRREEFVPAAYRA
                     LAFSDLEVPLRAKAGPAEHMLQPKVEARILQEIAPRKHETALEVGTGSGYFAALLGAR
                     SQRVHTVEIDPELKAFGEDNLRRAGVANVRVELGDAAQGWAALAPYDVIVLTASTPVL
                     TPSLLEQLKPGGRLFAVVGDPPVMEACLVRCLEPGAFQTVNLFETCLAPLRNAPQPRR
                     FTF"
     gene            14071..14394
                     /locus_tag="VI319_01610"
     CDS             14071..14394
                     /locus_tag="VI319_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6862569.1"
                     /translation="MQQITPQQLADWLADPSRGKPVLLDVREPWEFEICRIAASVHIP
                     MASVPGRKDELDPGADTVVVCHHGARSFQVGVLLEAAGFSRVYNLTGGVDGWARQVEP
                     GMPQY"
     gene            14398..15744
                     /locus_tag="VI319_01615"
     CDS             14398..15744
                     /locus_tag="VI319_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY6862570.1"
                     /translation="MLRITPRALVLLAVGCIGCAADAGAADLLQTYREALANDPQYAS
                     ARAAVEAGREKLPQGRALILPTLNATANTTYNDATIRPRTDPTSDIQRQFNSNGWTVT
                     LSQPLFRFQNWVQYTESEFQVRQSEAQFSQAVQDLIVRVAQAYFDVLAAQDTLAVIGA
                     QKAAISEQLAQAKRNFEVGTATITDTHEAQARYDLTVSQEIAAQNDLEIRRRTLQQLI
                     GRFPDRLTPLRSKLELRVPEPNDMERWVDSAQSQNYAVVVQRAGLEIAEREIDRNRAG
                     HYPTLDVVGTYGQTGSGASTNVNSGLGSDVKIGTIGLQLAIPLYSGGAVNSRTREAIA
                     NREKARADLETSRRQAALSARQSFLGVTNGMAQVRALEQALVSSQTALASNKLGYEVG
                     VRINIDVLNSQQQVFQTQRDLSRARYDTLLNGLRLKAAAGVLSDADVEDVNRLLGE"
     gene            complement(15773..17026)
                     /gene="waaA"
                     /locus_tag="VI319_01620"
     CDS             complement(15773..17026)
                     /gene="waaA"
                     /locus_tag="VI319_01620"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEY6862571.1"
                     /translation="MWPAGYRLLLWLAFPWILVRLWWRGRDEPGYRRNVAERFGFYRD
                     APSRPVLWLHAVSLGETRAAEPLVRALLARHPECELLVTQMTATGRAAAQALFGDGVR
                     LAYLPYDYPSAVRRFLRHYRPRVGLLMETEVWFNLVRECRAAGVPVLLVNARMSEKSA
                     RAYGAVAPLSRLAFAALRAVAAQTAEDAARLHALGAGAVEVTGNLKFDAVPATGMKEL
                     ASELRVRFGERRVLLAASTREGEEELLLDALERNSIGDALTVIVPRHPTRFDEVAAML
                     ARRGLAVVRRSAGAAVPADCRYVLGDSMGEMAAYYQACDVAYVGGSLLPYGGQNLIEA
                     CALGVPVLIGPHTYNFAQAAEAAVAEGAALRVASADDAIVRSRALLQDPDRRRRMAEA
                     GVRFCLAHRGATERTVELVERVVAG"
     gene            complement(17026..17973)
                     /gene="waaC"
                     /locus_tag="VI319_01625"
     CDS             complement(17026..17973)
                     /gene="waaC"
                     /locus_tag="VI319_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEY6862572.1"
                     /translation="MKKILLVKTSSLGDVIHNFPVATDIRRQLPEARIDWLVEEPYAA
                     LVALHPAVHRVIPVAIRRWRRGLLGHRTWKEIAEFRRLSQAESYDAVVDTQGLVKSAV
                     LARAARGRRHGFDAASAREPLAARLYDVTHHVARAQHAVRRNRLLAAAALGYRVGEGF
                     DYGIVAPAAAAREPYCVLLHGSSRVDKLWPETSWIELGRRLEARGMRCLLPWGSDEER
                     ERSERIATGLGRADVPAAMPLDRLAGALRGASAVAGVDTGLTHLAAALSRPVAAIYCA
                     TDPRLTGVVGGPATRNVGGPGHVPSAEEVWEALADVGAV"
     gene            18073..>18673
                     /locus_tag="VI319_01630"
     CDS             18073..>18673
                     /locus_tag="VI319_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6862573.1"
                     /translation="MALSAVIITKNAAAQLGECLDSVAFADETLVVDSGSTDATRELA
                     RARGARVLEHAWLGFGPQKQLAVAAAAHDWVLCVDADERVSPALREAIARELAAPRGR
                     VYALARCNRFLGRWLRHGEGYPDWSVRLFHRAAARWSDDAVHEKVLTAEAVQRLSGDL
                     MHDSAETLERYLDKQNRYTTLQAETLRAAGARASLAHLAL"
BASE COUNT         2978 a         6393 c         6424 g         2878 t
ORIGIN      
        1 tcgcgctgtt cgacatgcac ctgcaccacg atttcgatcc gctgaacgaa aatgaggaaa
       61 gcaagacgcc gctgcagctc ctcgaccaga tccgcgcgcg cgtcgcggtc gcgctgccgc
      121 cccggtacaa ccgcttcccc aacaatttct cgcatatctt cgcgggcagc tacgcggccg
      181 gctactacag ctacaagtgg gccgaggtgc tgtcggccga cgcctacagc atgttcgagg
      241 agcggcgcga ctccgccggc gtgctcgatc ccgagacggg cgcgcgcttt cgcgacgaga
      301 tcctcgccgt tggcgggagc cgtcccgcga tcgagtcctt caaggcgttc cgcggccgcg
      361 cgccgcgcat cgatgccctg ctgcgtcaca atggtatgat tgcggcatag cgcgcggcgg
      421 cgaagcagcg atctcgcgga cgcggcgcag cccggccggt ggccggcgcc acgaaggaaa
      481 tgccatgcgg atcgtgctcc tcgccggaat catcgtcctt gccgtttcgt ccgcgtacgg
      541 ccagatgtac cgctgggtcg acaaggacgg ccgtactcac tacacgcaga cgccgccgcc
      601 gcccgacgcc cgcagcgtgg agaagaagtc tctcgatgcg ggcgccggtt ccgccgctcc
      661 cggcgcgatg ccttacggcg agctgccgta cgcgacgcag aatgccgcgc gcaactaccc
      721 ggttacgctc tacaccagcc cgaactgcgg ggcgccttgc gagcaggcgc gcgcggcgct
      781 cgtccggcgt gcggtcccct tcaaggaagt cagcgtagcg gggcaacagg acatcgagaa
      841 tctcaaggct gcgggcggaa cggggctgcc ccatctcgtg gtgggaaacc tgaagcagac
      901 cgggttctcc gaggattcct acaaatccac tctcgacacc gcgggctacc cgcagtccgg
      961 gccgcgcacg tcgatcgagg ccttgcgcac catggacgcg ccggcgaaga cgcccgcccc
     1021 gggaggcggc ggcagcagcc aggaggcgcc caggtaagaa ggcggcggcg cgctgcgccc
     1081 tcgcgctttt cgattccaga accacaagag gagaaggcat gaggatggag acttcgtttt
     1141 tgcgcgccgc gtgcgcggcg gccgcggcgc tcgcgtcgtg cgccgcgctc gctcaggcgt
     1201 acccgaccaa gccggtgcac ttcgtggtgc cgttcgcggc gggcggaccg gccgacatct
     1261 acgcgcgatt cctgggcaac cggctggaga aaacgttggg gcagcctttc gtcatcgaag
     1321 accgccccgg cggcggcgcc atcatcggaa ccgaggtcgt ggtgaagtcg gcgcccgacg
     1381 gctacacgat cctcgcgatg tcgaacacac ataccgtgaa cgagtcgttg ttcaccaaga
     1441 agccgtacaa gctgatgcag gacctgacgc cgatcgcccc gatcaattac tcggacctgc
     1501 ttctcgtcgt caatccgagc gtgcccgcga agaacctgaa ggagctgatc gcgctcgtga
     1561 agtcgaagcc gggccagctg aattacgcgt cttcgggacc gggcacgccg tatcacatgg
     1621 cgggcgagct gttcaaagcg atggcgggcc tggatatcgt ccacgtgccg cacaagggca
     1681 gcgatcaggc gcgcaccagc gtgatggcgg gccagaccca gatgatgctc gacgccgtga
     1741 ccaccatggc gcagatcgcg cgctcgggca aggtgcgcgc gatcgcgacg accggcaaga
     1801 cgcgctcgtc gatcatgccc gaggtcccga ccatggacga ggccggcgtg cccggctacg
     1861 aggccaccat ctggctgggc gtaatggccc ccgcggggac gcccaagccc atcatcgaca
     1921 agctgaacgc ggagatccgc aagacggtcc accagcccga cgtcaaggac gcctgggcca
     1981 agcagggcgc cgtggcgatg gacatgacgc cgcagcaatt ctcgaagttc ctgcaacagg
     2041 acatcgagaa atgggcgaag gtggtgaaga tctccggggc gaaggtcgac tgagcggccc
     2101 gggccgcccg gtctcagtcg atcatcgcgt aggcgggcag cgtgaggaac tcgacgtagc
     2161 ggtcgtcgtt cgtgatgcgc tcgaagaggt cggccgcgtc ttcgtagcgc gccgcccgcc
     2221 atgcggcctc gccgagggtc tcgcgcacct tcgacagctc ctcgcgcagc agcgcgctga
     2281 acagctcctt ggtgaccttg cggccgtcgt cgaggacgcc gtgatcggag cggatccact
     2341 gccagatctg cgagcgcgag atctcggccg tcgccgcgtc ctccatcagg tggaacaccg
     2401 gcacgcagcc gttgccggcg agccaggcgc cgaggtactg gatgccgacg ctgatgttgt
     2461 tgcgcagccc cgcctcggtg atcggcttct cgggctcgaa gcgcagcagg tcggcggcgc
     2521 tcgcctgcac gtcggggcgc tgccggccgt actggttcgg ctgcggcatg tgctggtcga
     2581 agatctcctt cgcgatcggc accagcccgg gatgcgcgac ccaggtgccg tcgcagccgt
     2641 cggtcacctc gcgcagcttg tcctggcgca ccttctccat tgccgcggcg ttcgcagccg
     2701 ggtcgtcctt gatcgggatc tgcgccgcca tgccacccat ggccggagcc ccgcggcggt
     2761 gacaggtctt cacgagcagc agcgcgtacg cgcgcatgaa aggcgaggtc atcgtcacct
     2821 gcgcgcggtc ggcgagacag aaatcgccgt gcgagcggaa tttcttgatg caggaaaaga
     2881 tgtagtccca gcggccgatg ttcaggcccg cggagtgctc cttcagctcc cacaggatct
     2941 cgtccatctc gaaggccgcg agcacggtct cgacgagcgc ggtcgccctg atcgagcctt
     3001 gcggaacgcc gagggcgctt tgcgcgcgct tgaagatgtc gttccacagg cgcgcctcga
     3061 ggtgcgactc catcttcggc agatagaagt acgggccggt gccgcgagca agcagctcct
     3121 tcgcgttgtg gaagaagtag agcgcgaaat cgaagatcgc gcccgagacg ggacggccgt
     3181 cgacgtggac atgcttctcg tcgaggtgcc agccccgcgg ccgcgggatc agcaccgcag
     3241 tccggtcctt cagccgatag tgcttgccgc cctgctcgaa gctgatcgtg cggcggaccg
     3301 cgtcgcgcag attgatctgg ccttccacca tgttggccca ggtcggacag ttcgcgtcct
     3361 cgaagtcggc catgaacgtc gaggcgccgg agttgagcgc gttgatgacc atcttgcggt
     3421 cggtggggcc cgtgatctcg acgcgccggt cgagcaggtc ggccggctgc gcggcgatcg
     3481 accattcgct ggtgcggatc gatgccgtct ccggcaggaa atcgggcatc gcgcccgcgt
     3541 cgaagccccg ctgacgctcg gcccggcgcg agaggagctc ctgccgctgg ggctcgaatt
     3601 ctctgtgcaa tgcagcgaga aactcgagag ccgctgatgt cagtacttgc tcataaccgg
     3661 ctgtgaccgg tgccaggatc tcaataccct gggaagaatg gcttttcttg acggtggcgg
     3721 tcttggccat ggcgctgctc ctcggcgagt gcgacggact ttattgctgc gtcgcagcat
     3781 accagtaaat ctccgacgac gcttgctatt gcgccgcgcg gtgcggatgg gccgaacggc
     3841 ttaagggtag gaacgcgcgc gtaccggctc ggttcccgcg cgagcgcgtt atcgctcggg
     3901 cggctcttcg ccttcgatcc cgagctcggc tatcttgcgg gtaatcgtgt tgcggccgat
     3961 gccgagcagg gccgccgctt ccatgcgccg gccgccggtg tgggcgagcg tcttggcgat
     4021 gagagtcttc tcgaacgtgc gcagcagcgc gctcatgatg tcggcgtcgc cgcgcgcgag
     4081 ccggcgttcg gtttcgcgcg cgagcgcccc gagccagtcg ggctcgggtg cggccggatc
     4141 ctgccgcagc tcgggcggga gatcgctcac ctcgatgatc tgggtcggcg ccatcacggt
     4201 gagccagtgg catacgttct cgagctggcg cacgttgccg ggcaggtcct gcgcggcgag
     4261 gtatttcatc gctgcgtcgg agagacgctt cggctcggca ccgagatcgc gcgccgactt
     4321 ggcgaggaaa tggcgcgcga gcagcgggat atcgtcacgg cgctcgcgca ggctggggag
     4381 ccgcagccgg atgacattga ggcggtggta caggtcttcg cggaaggcgc cgtcctgcac
     4441 gcgctgctcg atttcctggt tggtggccgc gatcacgcgc acgctcgcgc ggatcgactg
     4501 gtgcccgccg acccggtaga aggtgccgtc cgagagcaca cgcagtagcc gcgtctgcag
     4561 ctcggccggc atgtcgccga tctcgtcgag gaacagcgtc ccgccctcgg cctgctcgaa
     4621 gcgcccgcgc cgcatcgact gcgccccggt gaaggcaccc cgctcgtggc cgaagagctc
     4681 cgactccagc agatccttcg gcatcgccgc ggtgttgatc gcgacgaagg gattgcccgc
     4741 gcgcgggcta tggcggtgca gagcgcgggc gaccagctcc tttccggtgc ccgactctcc
     4801 ggtgatgagc accgtcgccg atgactgcga gagccggccg atcgcgcgaa agacctcctg
     4861 catcgacgcc gcctggccga ggatttccgg cgtcgccccg ggagcctcct cggcttcccc
     4921 ctcgcgcagc gattcgtcga gcgcgcgccg gatcagctcg accgcttggt cgacgtcgaa
     4981 aggcttcggc aggtactcga acgccccgcc ctggaacgcg gcgaccgcgg attcgaggtc
     5041 cgagtacgcc gtcatgatga tcacggggag attcgggtag cgctgcttga cgttctgcag
     5101 caggtccagg cccgatgctc ccggcatgcg gatatcggag acgagcacct gcggcgcccc
     5161 gaccgatagc gcgtcgagcg cctcggtggc ctgtgcgaag gtcttgaacg gaatgccttc
     5221 gcgcgtcagc gccttctcga acacccagcg tatcgagcgg tcgtcgtcga tgacccagac
     5281 gggcttcacg acttcactcc gcgctcaccg cagcggcagc gtgatccgaa aacatgtgct
     5341 ccccggctgg ctttcgcatt cgatgacgcc gtgatgatgc tgcacgaaag tctgggccag
     5401 cgtcagtccg aggcccgtgc ccccctcccg cccggacacg agcggatgaa agatgcggtc
     5461 ccggatctcg gccggaacgc ccggcccgtc gtcgattact tgcaattcta atgccagccc
     5521 gtgaccgcgc ttgaccagcg aaacctgccg cgacacgcgg gtgcgcagca cgatctcacc
     5581 gccgtgtctg gccgggtccg cggcaatcgc ctgcgccgcg ttgcgcgcga tgttcaggac
     5641 cgcctggatg agctgctcgg gatcgccacg gaactcgggc aggctcacgt cgtagtcgcg
     5701 ccggatgccg atgccgtgcg ggaactcggc gaggatcagg ctgcgcacgc gctcgcacac
     5761 ctcgtggatg ttcatgggca cgtgcgtggg ggggcggtgc ggcgtgagca ggcggtccat
     5821 cagcgtctgc agccggtcgg cctccttgat gatcacctgc gtgtactcgc gcagctcgag
     5881 ccggtcgagc tcgcgctcga ggagctgggc cgagccgcgc aggccgccga gcgggttctt
     5941 gatctcgtgc gcgagattgc ggatcagctc gcggttcgcc atgtgctgcg tcagccgctg
     6001 ctcctcacgc tcgatgcgaa ggcgctggtc gatgggtcgc agctcgagga gcacgcccgg
     6061 ccctccgctc tcgacgggac tcgcgaggca attgagatgc acgggctcct ggccgatggg
     6121 cgcgagcacg aggtcgggat cggtgaagcc gcgctcgtcg cgcagcgcct gccgcagctg
     6181 ggcctcgagc gcctgtgcgt cggcgaacag ctcgtcgagc cggtggccga gcatgctgcg
     6241 ctgggcgagc gcgaacgtcg cttcggccgc gggattggca tagcgcacga ccaggccggc
     6301 gtcgagccag accacggcgg tggcgagcag atccaggccc tggaaagcgg ccgtcgtcat
     6361 gacacgtgcg ctgcgcgccg gcgggcgccg gcgcgcgcgg aaaggcggct tagcggtagt
     6421 tgccgagctc gtgctgcagc tgctcgatgt tcttctgatg caggtcgacg gtttcctgga
     6481 agggcttcag ccggtcctcg acgcgcgcat agttgcgctc gtcgccgctg cgctgtccct
     6541 gttgctcggc gagcttctgc ttcgcgtcgc cgagcaagcg ctgctcggac tcgagctcgt
     6601 tctcgaggat cttccggcgg ctctcgtcgc gcgagagcat cgcgccgccg ctcgccttcg
     6661 cgggcaccgg acgcgccacg gggatggccg gaaccacaga gacctcgcgc gtcaccatgg
     6721 tgcagccctt ccccacggtg tcgcgcttga cgttcgtata ggtcgggcga cccatcgcgt
     6781 ccggacagcg atagatctcg ccctgcgcct gggcccaacc gcaccaggac gcaataatcg
     6841 tcatagaaat caatttgttg cgtgtattca tgctcacaac cctccccgaa ggggaattga
     6901 agtctaggcg aaatgggctc tgttttcaaa tgaaaaaagg acgggggctc atacgaaacc
     6961 cccgtccttc ctgggccgtt cggaccagcg cccagcgtct caggcgctgt aatacatgtc
     7021 gaactcgacc gggtgcgtcg tcatgcggaa gcgggtgacc tcttccatct tgagcgcgca
     7081 gtacgcatcg agcatatcgt cggagaagac gccgcccttg gtcaggaatt cgcggtcctt
     7141 gttcaggtgg tcgatcgcca tgtcgagcga cgagcacacg ttctggatct tcttcgcctg
     7201 cgcgggcggc aggtcgtaca ggttcttgtc gaccgggtcg ccggggtgga tcttgttctg
     7261 cacgccgtcc aggcccgcca tcagcatcgc ggcgaaggcg aggtatgcgt tcgaggtcgg
     7321 gtccgggaag cggacctcga cgcgccgcgc cttcgggttg gtgacgagcg ggatgcggca
     7381 cgcggccgag cggttgcgcg acgagtacgc gaggttgatc ggcgcctcga atcccggcac
     7441 caggcgcttg tacgagttcg tgcccgggtt ggtgatcgcg ttcagcgcct tggcgtgctt
     7501 gatgatgccg ccgatgtagt agagcgcgaa gtcggacagc cccgagtagc cgttgccgct
     7561 gaacaggttc tgcccgcctt tccacaccga ctggtgcacg tgcatgcccg acccgttgtc
     7621 gccgacgatc ggcttcggca tgaaggtcgc ggtcttgccg tacgagtgcg cgacgttcca
     7681 gatggtgtac ttcaggatct gcatccagtc ggcgcgcttc accaggctgt tgaacttggt
     7741 gccgatctcg cactggcccg cggccgcgac ctcgtggtgg tgcacctcga cttccacgcc
     7801 ctgctgctcg agcgcgagcg agatcgcatt gcggatgtcc tgcagcgtgt cggccggcgg
     7861 cacggggaag tagccgccct tcaccggagc gcgatggccc atgttgccgc cttcgtactc
     7921 ctcgcccgag gaccacggcg cctcttccga cttgatcttc acgaagcagc ccgacatgtc
     7981 gacgttccag gtgaccgagt cgaagatgaa gaactcgggc tcgggcccga agtacgcgac
     8041 gtcgcccagg ccactcgact tgaggtacgc ctcggcgcgc ttggcaatgg tgcgcgggtc
     8101 gcggtcgtag cccttgccgt cagcgggctc gaccgcgtcg caggtgaggt tcagcaccgg
     8161 ctcgtcggtg aagggatcca tgcgcgccga gtccggatcc ggcatgagca gcatgtcgga
     8221 ggcctcgatg cccttccagc ccgcgatcga ggagccgtcg aagaagtgtc cgctctcgaa
     8281 cttgtccatc ccgaaggact tcaccggcac cgagacgtgc tgctccttcc cgcgggtgtc
     8341 cgtgaaacgg aggtcgacga acttcacctc cttgtccttg atcagtttca tgacgtctgc
     8401 ggcagacatt gccatttgat ggtctcctcg agtgcggatt gtcgtgatgg attggaagcg
     8461 cccggacggc gaaacggccc aaaaacaaaa aacagggcgc taaatcagca gaatttgtgc
     8521 catccggttc gaaaaggcag gccattgtgc cataacggtg tagcgcggga catgggcgca
     8581 ccggggcggg gcgctcggca ctccagccgc accaaccggg tgcaggactc accaccgtcg
     8641 cggcgcgcgc cttatactcg tctcatggac tcgcccgacg acatcctcgc ccgcgcgcgc
     8701 gagcgcgccc gttcgatgaa gctgccttac gcgggtgcgc tgcttccggc cgaagcgcac
     8761 gccctggtca cgaaagccgc ggacgccaag ctgatcgacg tccgcaccaa ggcggagctg
     8821 gagtgggtgg gacgcgtccc cggagccgtg acgatcgaat ggaacacctg gcccggcggg
     8881 gcgcagaacc cgaatttcct gcgcgagctg caggaccagg tcaaggcgaa ggacgcgccg
     8941 gtcctgttcc tgtgccgctc gggcgggcgc tcgcacaacg ccgcggccgc ggcgacgcag
     9001 gccggctatg ccaacgcctt caacgtgctc gagggattcg aaggcgacaa ggacacgcag
     9061 ggacatcgca acaccgtcgg cggctggcgc cgggcggggt tgccctgggt gcagggctga
     9121 gagcgcccgt ccgcatcgaa aaacaagtca gttcattacg gaggataaca acatgaagca
     9181 gctatccggt tcggcggtcg cggcgttggc gtgcgcggcc tggctgttcg cggcacccgc
     9241 tacccaggct caggagttca ggatcctgca gccgctgggc gacaagccga tgaagatgaa
     9301 aaacggcgac tactacgtgc cgaccacccc cgacaccgcg atgtggggca gcctgccgaa
     9361 caccaatacg aagccggtgc tctcggtgcc ttcgggcagc gtcgtgacga tcgacaccgt
     9421 ctcgcacgag ggcgtgctcg aggatcaggg caaggacccg gtgaagttct tcgggcagtt
     9481 cggcgtcaag cccgcccagg tgctcaagga cgcgcaggcc atcgccgcct ccagcatccc
     9541 gcacgacttc gtcaaggacg gcccgcacgt cgtcaccggg ccggtggagg tgcaaggtgc
     9601 gaaggtggga gacgtgctga tggtcgagat gatcgggctg cagccgcgcg tgccctacgg
     9661 cgtcatctcg aatcgccacg gcaagggcgc gctgcccggc gagttccccg agaaccaggg
     9721 acagcagccg ggcgcggaca cggcgcaccc cgagctctac gctaacgtgt tcaccttcgt
     9781 gccgatccgg aaaatcaacg gcacgtggta cgggatcgtg aagggcaaga gcggccccga
     9841 ggcgcatatc ccgttgaagc ccttcaacgg cacgatgggc gtcgccgtga acacgcagga
     9901 gaagccgaac tcggtgccgc ccggcgcgta cgcgggcaac ctcgatgtga acgagctatc
     9961 ggtgggggcc aagctgtaca tcccggtaca ggtggacggc gcgctgttct acgcggcgga
    10021 cccgcatttc gtccaagccg acggagaagt cgcgctgacg gccatcgaag ggtcgcttcg
    10081 ctcgacgtat cgcctgacgc tgctcaaggc cggcgacccg cgcctgccga tgaaggcgcc
    10141 gatgaagaat ccttttgcgg agacgaagga atattggatc ccgatcgggc tcaacacgga
    10201 tcttaacgag gcgatgaagg acgcgacgcg ccagaccgtg cagttcctga gtgagaagct
    10261 cggcatggac cgcgctacgg cgcttgcgta tgcgagcgcc ggagtggact tccaggtgac
    10321 gcaggtcgtg gaccgcgtga aaggcgtcaa cgcgatcatc cgcaagtcgg acttcgcttc
    10381 cgcccgcggc gcgggcacga aaggcaagaa gaagtagcgg cggccggcgg ggcctacccc
    10441 gccgaccagt tcaccacgcc gccccacgcc gtcgcaagca cgaccgcgcc gaacgcgatg
    10501 cggtaccacg cgaacggcgt gaagtcgtgc gtggcgatgt agcgcagcag ccagcgcacg
    10561 cacaggaacg ccgagacgaa cgccgcgacc gatccgaccg cgaagacgcc gaggtcgccc
    10621 gccgagagca gcccccggtt cttccagaga tcgtagacgc cggctgcgac cagcgtcggc
    10681 accgcgagga agaacgagaa ctcggtcgcc gcgcgccgcg acaagccgaa cagcatgccg
    10741 ccgatgatgg tcgcgcccga gcgcgaggtg cccgggatca gcgcgaaggc ttgggcgacg
    10801 cccaccttga gcgcatcgct ccagcgcatc gcgtccaccg agtcgacgcg cacccgcggg
    10861 cgcagcgcgc gctcgaccca aaggatgatc acgccgccga cgatgaacgc gagcgcgacc
    10921 gggacggcgt gaaagagatg cgtcttgatc gcgttgtgga acgcgagccc gagcagcgcc
    10981 gccggaagga aggcgatgag caggttgacg gcgaagcgct gcgcgccgcg gtccgacgga
    11041 agtcccgcga gcacgcgcca gaacttcagg cggtactccc agacgatcgc gagcatcgcg
    11101 ccggtctgga tcacgatctc gaacaccttc gccttctcat cgctgaaacg caggagctcg
    11161 cccgcgagga tgagatggcc ggtggaggag atcggcagga actcggtcag cccctcgacg
    11221 acgcccatca ccaaggcctt caccagcaga acgacgtcca ccgggcctcc tgcttcacgt
    11281 cttgtggtag atctcggcgc ccttcttcac gaactcgatc gccttctcgc ccatgccgcg
    11341 cttgagggcg tcgtgctccg agaccccgtg cttctcggcg tagtcgcgca cgtcctgcgt
    11401 gatcttcatc gagcagaagt gcggcccgca catcgagcag aagtgcgcga gcttcgcgcc
    11461 ttcctgcggc agcgtctcgt cgtggaactg cttcgccttg tcggggtcga ggcccaggtt
    11521 gaactggtcc tcccagcgga actcgaagcg cgccttggac agcgcattgt cgcggatctg
    11581 cgcgcccgga tggcccttgg cgagatcggc ggcgtgcgcg gcgatcttgt acgcgatgat
    11641 gccgtccttg acgtcttcct tgtcgggcag ccccaggtgc tccttcggcg tcacgtagca
    11701 gagcatcgcc gtgccgtacc agccgatcat cgccgcgccg atcgcgctcg tgatgtggtc
    11761 gtagccgggg gcgatatcgg tggtgagggg tccgagcgtg tagaacggcg cctccttgca
    11821 gtactcgagc tgcagctcca tgttctcctt gatgagctgc atcggcacgt gcccggggcc
    11881 ctcgatcatc acctggcagt cgtggcgcca cgcgacgtcg gtcagctccc ccagcgtctt
    11941 cagctccgcg atctgcgcct cgtcgttggc gtcgtagatc gagcccggcc gcaggccgtc
    12001 gccgagcgag aacgacacgt cgtacgcggc catgatctcg cagatctcct cgaagtgcgt
    12061 gtagaggaag ctttcctcgt ggtgcgcgag gcaccacttc gccatgatcg agccgccgcg
    12121 ggagacgatg cccgtcatgc gcttcgccgt gagcgggatg aacggcagcc gcacgccggc
    12181 gtggatggtg aaatagtcga cgccctgctc ggcctgctcg atcagcgtgt cgcggaagat
    12241 cgcccaggtc agttcttccg ccttgccgtc gaccttctcg agcgcctggt agatcggcac
    12301 cgttccgatc ggcaccgggc tgttccggac gatccactcg cgcgtctcgt ggatgtgcct
    12361 gccggtcgag aggtccatca ccgtgtcgcc gccccagcgg atcgcccagg tcatcttctc
    12421 gacttcctcg gcgatgcccg aggtgacggc ggaattgccg atgttggcgt tgatcttcac
    12481 caggaaattg cggccgatga tcatcggctc gctctcgggg tggttgatgt tcgcggggac
    12541 gatcgcgcgg ccgcgcgcga cctcgtcgcg cacgaactcc ggcgtgatca gcctctggat
    12601 cgaggcgccg aacgactcgc cgggatgctc ccgggtgatc agctcggaga gcgcgtcgcg
    12661 ccgctggttc tcgcggatcg cgatgtactc catctcgggt gtgacgatcc cctgcttcgc
    12721 gtaatgcatc tgcgtgacgc agcgtccggc cttcgcgcgg cgcggcctgc gcttgagatc
    12781 gaagcgcaac tcggcgagct tgggatcggc gagccgctcg cggccgtagc tcgacgtggg
    12841 accggcgagc tcctcggtgt cgccgcgctc ggcgatccag cgcccgcgca aaggcgccag
    12901 cccggcgcgg atgtcgatcg cgacggccgg atcggtgtaa gggcccgacg tgtcgtatac
    12961 gtagatcggc gggttcttct cggcgccgaa ctgcgccggc gtgtcggact gccggatctc
    13021 gcgcatcggc acgcgcacgt ccgggcgcga cccctcgacg tagaccttgc gcgagctcgg
    13081 cagcggcttg accgcggctt cgtcgacgtg ggcggtcttc gctaggaact tgggattggc
    13141 attcatggcg cttcctctcg caatctggac tgccggggaa gcggaagcgg acttcaacgc
    13201 ttccctgcgg cggcattatc cgcatcaggt tcgaagggtc tttctcaccg aggccgggcg
    13261 accgcccatc caaggacccc tacgttgcgc tcacagttta gcaccgcgac aagaccgggc
    13321 gcgcgcgccg cgcgttcacg ggaaagccga tgaatcgatg ctagaatcca cgccgtcgca
    13381 ccgccacccg gcaccccatg aacgtcgagc aggcccgctt caacatgatc gagcagcaga
    13441 tccgcacttg ggaggtgctc gatcaggatg tgctcgacct gctctacgcc gtgcgccggg
    13501 aagaattcgt gccggcggcg taccgcgcgc tcgcgttctc ggacctggag gtcccgctgc
    13561 gcgccaaggc cggtccggcc gagcacatgc tgcagcccaa ggtcgaggcg cgaatcctgc
    13621 aggagatcgc tccgcgcaag cacgagaccg cgctcgaggt cggcaccggc agcggctact
    13681 tcgccgcgct gctcggcgcg cgctcgcagc gcgtccacac cgtggagatc gacccggagc
    13741 tgaaggcgtt cggcgaggac aacctgcgcc gcgccggcgt cgcgaacgtc cgcgtcgagc
    13801 tcggcgacgc cgcccagggc tgggcggcgc tcgcgcccta cgatgtcatc gtcctcacgg
    13861 catccacgcc cgtgctgacg ccgtcgctgc tcgagcagct caagcccggc ggccgcctgt
    13921 tcgcggtggt cggcgacccg cccgtcatgg aggcttgcct cgtgcgctgc ctcgagccgg
    13981 gcgcgttcca gaccgtgaac ctgttcgaaa cctgcctcgc gcccctcagg aacgcgcccc
    14041 agccgcggcg tttcacgttc tagcggcgac atgcagcaga tcacgccgca gcagctcgca
    14101 gactggctcg ccgacccgtc gcgcgggaag ccggtcctgc tcgacgtgcg cgagccctgg
    14161 gagttcgaga tctgccgcat cgccgcttcg gtgcacatcc cgatggcgag cgtccccggg
    14221 cgcaaggacg agctcgatcc gggcgcggat accgtggtcg tgtgccacca cggcgcgcgc
    14281 agcttccagg tcggcgtgct gctcgaggcc gcgggcttca gccgcgtgta caacctgacc
    14341 gggggcgtgg acggctgggc caggcaggtc gagcccggca tgccgcagta ctgagcgatg
    14401 ctccgcatca cgccccgcgc gctcgtcctc ctcgccgtcg gctgcatcgg ctgcgccgcc
    14461 gacgccggcg cggccgacct gctccagacc taccgcgagg cgctcgcgaa cgacccgcag
    14521 tacgcgtcgg cgcgcgcggc ggtcgaggcc ggacgcgaga agctgccgca gggacgcgcg
    14581 ctcatcctgc cgaccctgaa cgcgaccgcg aacaccacct acaacgacgc gaccatccgc
    14641 ccgcgcaccg acccgacgag cgacatccag cgccagttca acagcaacgg ctggaccgtg
    14701 acgctgagcc agccgctgtt tcgtttccag aactgggtgc agtacacgga gtcggagttc
    14761 caggtgcgcc agtcggaggc ccagttcagc caggcggtcc aggacctcat cgtgcgcgtg
    14821 gcgcaggcgt acttcgacgt gctcgccgcg caggacacgc tcgccgtgat cggcgcgcag
    14881 aaggcggcga tctccgagca gctcgcgcag gcgaagcgca acttcgaggt cggcaccgcg
    14941 accatcaccg acacgcacga ggcgcaggcg cgctacgacc tgaccgtctc ccaggagatc
    15001 gcggcgcaga acgacctcga gatccggcgg cgcacgctgc agcagctgat cggccgcttt
    15061 cccgaccggc tgacgccgct gcgatcgaag ctcgagctgc gcgtcccgga gccgaacgac
    15121 atggagcgct gggtcgactc cgcgcagagc cagaactacg ccgtcgtggt gcagcgggcc
    15181 ggcctcgaga tcgccgagcg cgagatcgac cgcaaccgcg ccgggcatta cccgacgctc
    15241 gacgtggtcg gcacctacgg ccagacgggc tcgggcgcga gcaccaacgt caactcgggc
    15301 ctcggcagcg acgtcaagat cggcaccatc gggctgcagc tcgcgatccc cctctactcc
    15361 gggggcgcgg tgaactcgcg cacccgcgag gcgatcgcca accgcgaaaa ggcgcgcgcc
    15421 gacctcgaga cgagccggcg ccaggcggcg ctctcggccc gccagtcctt cctcggcgtc
    15481 accaacggca tggcgcaggt gcgcgcgctg gagcaggcgc tcgtgtcctc gcagaccgcg
    15541 cttgcgtcga acaagctcgg ctacgaggtc ggcgtgcgca tcaacatcga cgtgctgaac
    15601 tcgcagcagc aggtcttcca gacgcagcgc gacctgtcgc gggcgcgcta cgacacgctc
    15661 ctcaacggcc tgcgcctcaa ggcggccgcc ggtgtcctga gcgacgcgga cgtcgaagac
    15721 gtcaaccggc tgctcgggga atgacgtcgg ggcacggccg ccccgacggc gactagccgg
    15781 cgaccacgcg ctccacgagc tccacggtgc gctcggtcgc cccgcgatgc gcgaggcaga
    15841 agcgcacgcc ggcctccgcc atgcgccggc gccggtcggg gtcctgcagc agcgcacgcg
    15901 agcgtacgat cgcatcgtcg gcgctcgcca cgcgcagcgc cgccccctcg gcgaccgcgg
    15961 cctccgccgc ctgcgcgaag ttgtaggtgt gcgggccgat cagcaccggg actccgagcg
    16021 cgcacgcctc gatcaggttc tgcccgccgt acggcaggag gctgccgccc acgtacgcga
    16081 cgtcgcacgc ctggtagtac gccgccatct cgcccatgct gtcgccgagc acgtagcggc
    16141 agtcggcggg gacggcggcg ccggcgctgc ggcgcacgac cgcgagcccg cggcgcgcga
    16201 gcatcgcggc cacctcgtcg aagcgggtcg ggtgccgcgg cacgatcacc gtcagcgcgt
    16261 cgccgatcga gttgcgctcg agcgcgtcga gcagcagctc ctcctcgccc tcgcgcgtgc
    16321 tcgcggcgag cagcacgcgg cgctcgccga agcgcacgcg cagctccgag gcgagctcct
    16381 tcatgccggt cgccgggacg gcgtcgaact tcaggttgcc ggtgacctcg accgcgccgg
    16441 cgccgagcgc gtgcagccgc gccgcgtcct cggcggtctg cgcggcgacc gcgcgcagcg
    16501 cggcaaaagc gagccgcgag agcggagcaa cggcgccgta ggcgcgggcc gatttctccg
    16561 acatgcgcgc gttcacgagc agcaccggaa cgccggccgc gcggcactcg cgcacgaggt
    16621 tgaaccagac ctcggtctcc atgagcaggc cgacgcgcgg gcggtagtgc cgcaggaagc
    16681 ggcgcaccgc ggagggatag tcgtacggca ggtacgcgag gcgtacgccg tcgccgaaga
    16741 gcgcctgcgc cgccgcgcgg ccggtcgcgg tcatttgcgt gaccagaagc tcgcactcgg
    16801 gatggcgggc gagcagcgcg cgcaccagcg gctcggcggc gcgcgtctcg cccagcgaca
    16861 ccgcgtgcag ccagaggacc ggacgcgagg gcgcgtcgcg gtagaagccg aagcgctccg
    16921 ccacgttgcg gcggtagccc ggctcgtcgc ggccgcgcca ccaaaggcgg accaggatcc
    16981 agggaaacgc gagccacagc agcagccggt agcctgcggg ccacatcaca cggccccgac
    17041 gtccgccagc gcctcccaga cttcttcggc gctcggcacg tggccgggcc cgccgacgtt
    17101 gcgcgtcgcg ggaccgccga cgacgccggt cagccgcgga tcggtggcgc agtagatcgc
    17161 cgccaccggc cgcgacagcg cggcggcgag atgcgtcagc ccggtgtcga cgccggcgac
    17221 ggcgctcgcg ccgcgcagcg cgcccgccag ccggtcgagc ggcatcgcgg ccggcacgtc
    17281 ggcgcggccg aggcccgtgg cgatgcgctc gctgcgctcg cgctcttcgt cgctgcccca
    17341 gggcagcagg cagcgcatgc cgcgcgcctc gaggcgccgg ccgagctcga tccacgacgt
    17401 ctcgggccag agcttgtcca cgcggctcga gccgtgcagc agcacgcagt acggctcgcg
    17461 cgcggcggcc gcgggcgcga cgatgccgta gtcgaagcct tcgccgacgc ggtagccgag
    17521 cgccgccgcc gcgagcagcc ggttgcggcg caccgcgtgc tgcgcgcggg cgacgtggtg
    17581 cgtcacgtcg tacaggcggg cggcgagcgg ctcgcgtgcg ctcgcggcgt cgaagccgtg
    17641 gcggcgcccg cgcgcggcgc gcgcgagcac cgcgctcttc accaggccct gggtgtcgac
    17701 gacggcgtcg taggactcgg cctgcgacag gcggcggaac tcggcgatct ccttccaggt
    17761 gcggtgcccc agcagcccgc ggcgccagcg ccggatcgcg accgggatga cgcggtgcac
    17821 ggcggggtgc agcgcgacca gcgcggcgta gggctcctcg accagccagt cgatgcgcgc
    17881 ctccggaagc tgccggcgga tgtcggtcgc gaccgggaag ttgtggatga cgtcgccgag
    17941 cgacgacgtc ttcacgagca ggatcttctt catccccggg cgggcagccc gcgagcgcgg
    18001 aatagggtta gaatgacccg cataagctcc tgattatacg ggattgccgg cgtcgccggc
    18061 acgggaagat ccatggcctt gtccgcggtg atcatcacca agaacgccgc cgctcagctg
    18121 ggcgaatgtc tcgacagcgt cgcgttcgcg gacgagacgc tggtcgtgga ctccggctcg
    18181 accgacgcga cgcgcgagct cgcgcgcgcg cgcggcgcgc gcgtgctcga gcacgcctgg
    18241 ctcggcttcg ggccgcagaa gcagctcgcg gtcgcggctg ccgcccacga ctgggtgctg
    18301 tgcgtcgacg ccgacgagcg cgtgagcccg gcgctgcgcg aggccatcgc gcgcgagctc
    18361 gccgcgccgc gcgggcgcgt ctacgcgctc gcccgctgca accgcttcct cgggcggtgg
    18421 ctgcgccacg gcgagggcta cccggactgg agcgtgcgcc tgttccaccg ggcggccgcg
    18481 cgctggagcg acgacgcggt gcacgagaag gtgctgaccg ccgaagccgt gcagcggctc
    18541 tcgggcgacc tgatgcacga ctcggccgag acgctcgagc gctatctcga caagcagaac
    18601 cgctatacga cgctccaggc cgagacgctg cgcgcggcgg gcgctcgcgc gtcgctcgcg
    18661 catctcgcgc tct
//
LOCUS       DATDMW010000041         5948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2002014, whole genome shotgun sequence.
ACCESSION   DATDMW010000041 DATDMW010000000
VERSION     DATDMW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002014"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VI319_01635"
     CDS             <1..1196
                     /locus_tag="VI319_01635"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6862574.1"
                     /translation="GGVIGGVGAFQDMSERMRMEQAIRENERLLRGIFDILPVGVWIA
                     DRTGRIVSSNPAGERIWRGARYVPIAEFGQYKGWWADTGKPIAAEEWALARAITRGET
                     SDAELIRIQCFDGSFKTIINTAAPLRDEHGEIAGAIVVNEDITALQYTQEQLRAAVRD
                     REEILAVVTHDLRNPLNGLAAAAAALDRKLRELPGGAPLAPVAATLVDIARRMSGMVD
                     DLLAVAVTATGGPSMLKLEPVAAASLIDRAVQSARPLLAKGSLQLQVNVAGELPIIHA
                     DADRVLRVFANLLDNALKFTPPRGRITLSAERTAAGVRFCVDNTGPPLARAEIEAMFQ
                     PFWQATHDRRGAGLGLSICRSIVEAHGGSIWPEPVQGERVRVCFVLPRHPLTGRIAPP
                     PLPGP"
     gene            1255..1611
                     /locus_tag="VI319_01640"
     CDS             1255..1611
                     /locus_tag="VI319_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862575.1"
                     /translation="MYEHRTQPLLSRRKFFGRLAQSAAVAAGLAFVSLAIGMTGYHVF
                     ERLDWIDAFLNAAMLLGGMGPVDAPMTAAGKLFAGLYALYCGLVVIIATGVILAPLAH
                     RLMHRFHLEQERDRDH"
     gene            1611..2774
                     /locus_tag="VI319_01645"
     CDS             1611..2774
                     /locus_tag="VI319_01645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6862576.1"
                     /translation="MDFALTGEQQRIRDAVARLCTPFDAQYWLARDRDGAFPHDFHQA
                     FADAGWLGIAMPEQYGGAGLGIVEAALMMQAVSESGAGFSGASALHMNIFGLNPVVVF
                     ASEEQKRRWLPPLVAGRDRACFAVTEPDAGLNTLGLKTKALRDGDHYVVSGQKMWIST
                     AQVATKILLLARTTPVEEARGTHGLTLFYTDFDRGKIEARVIDKMGRHAVDSNQVFID
                     GLRVPAADRIGDDGRGFEYILHGMNPERILIAAEAVGLGRAALARAAAYANERIVFDR
                     PIGQNQAVQHPLARSWMELEAANLMTFNAASLYDAGKPCGAEANAAKYLAAEACWRAC
                     ETAVLTHGGMGYAKEYHVERYLREAWIPRLAPVSPQLILCFIAEKVLGLPKSY"
     gene            2970..3458
                     /locus_tag="VI319_01650"
     CDS             2970..3458
                     /locus_tag="VI319_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862577.1"
                     /translation="MVELLVRRAVALHQRTLAPQRQVGDRGEHLGGGVRLGGRAVDAH
                     APRAQRRGGLRPARGDDGLRERVEQLRRRAERVGRLEQRARPDAGLEHGQFRRAHTEP
                     VHEAPDRIEVDVGRLGERGRGDDARAAPGDQPGELRRQPALEDRDRTGFHCVDCSVRP
                     GD"
     gene            3501..4121
                     /locus_tag="VI319_01655"
     CDS             3501..4121
                     /locus_tag="VI319_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6862578.1"
                     /translation="MPTVLITGAGRGLGLEFARQYAADGWKVIATIRRSDAKATLQSL
                     GADVDGGVALDVLINNAGIYGPSGLAIGTLDYAAWEQVLRVNVLGPAAMSEALRDNVA
                     ASGRRIIVMISSRLGSIMEMRAGDYLYGSSKAALNAVTKSFASALGRGVIVVALSPGW
                     VRTDMGGAGAPLAPETSVAGMRKVIAGLSRRDSGRFINYDGSDIPW"
     gene            complement(4125..4577)
                     /locus_tag="VI319_01660"
     CDS             complement(4125..4577)
                     /locus_tag="VI319_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862579.1"
                     /translation="MKEILGVTDEPRTLRRWFHDDYFDLFVWQTGEGEITLFQLCYGI
                     DSSERALVWHQYGGFFHDGLHSARSSEQVLGARLHPDAPLTDDPIVARFESAANTLPD
                     DIRDFIAARLKEYAEQKPVPARRKRFRREEWQRKPGAKAAKSGKQQAS"
     gene            4843..5436
                     /locus_tag="VI319_01665"
     CDS             4843..5436
                     /locus_tag="VI319_01665"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862580.1"
                     /translation="MLLISVLAGTLNGKAALAQQQSPDQLVKAATEDVLAAIKKTTDR
                     ATLRKVAQQKVLPYFDFTEMTRLAVGRPWREATPEQKKKLQAEFTDLLVNTYTSALTR
                     TVAADKTVEVKPLQAGANDKEVTVRSLVKGGGGQPLDIDYRMTKEDQGWKVIDVIVAN
                     VSLVTTYRGSFAQEVQQGGIDGLIKSLEGKNRQLAKG"
     gene            complement(5459..>5948)
                     /locus_tag="VI319_01670"
     CDS             complement(5459..>5948)
                     /locus_tag="VI319_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6862581.1"
                     /translation="GTSSRLSVELFKDLTGIDPTAIHYKGGGPAAVALIAGQVDMMII
                     TLGVAIEHVRAGKLVAIAVTSPRRAPQLPDVPTLAETYPGFETQSWMGLLAPAGTPAA
                     IVDRLSGEIRKALAAGPSKEKLESFGYEVVASSPAEFGSFVRAESARWERLIRERHIT
                     ID"
BASE COUNT          901 a         2127 c         2055 g          865 t
ORIGIN      
        1 acggcggcgt tatcggcggt gtcggcgcct tccaggacat gagcgagcgc atgcgcatgg
       61 agcaggcgat ccgggagaac gaacgcctgc tgcgcggcat cttcgacatc ctgccggtgg
      121 gcgtatggat cgccgaccgg acgggccgca tcgtcagcag caatcccgcc ggcgagcgca
      181 tctggcgcgg cgcccgctac gtgccgatcg ccgagttcgg gcagtacaag ggctggtggg
      241 ccgataccgg caagcccatc gccgccgagg aatgggcgct ggcgcgcgcg atcaccaggg
      301 gcgagacctc cgacgcggag ctgatccgca tccagtgctt cgacggctcg ttcaagacca
      361 tcatcaacac cgccgctccg ctgcgcgacg agcacggcga gattgcgggc gccatcgtgg
      421 tgaacgagga catcacggcg ctgcagtaca cgcaggagca gctgcgcgcc gcggtgcgcg
      481 accgggagga gatcctcgcg gtcgtcacgc acgacctgcg caacccgctg aacggcctcg
      541 ccgcggcggc ggccgcgctc gatcgcaagc tgcgcgagct gccgggcggc gcgccgctcg
      601 cgccggtggc cgccacgctg gtcgacatcg cgcgccgcat gtcggggatg gtggacgacc
      661 tgctcgcggt cgcggtcacc gccacgggcg gcccgtcgat gctgaagctc gagccggtcg
      721 cggccgccag cctgatcgac cgcgcggtgc agtcggcgcg gccgcttctc gcgaagggat
      781 cgctgcagct gcaggtcaac gttgcgggcg agctgccgat catccatgcc gacgccgacc
      841 gggtcctgcg ggtgttcgcg aacctgctcg acaacgcgct caagttcacg cccccgcgcg
      901 ggcgcatcac cctctctgcg gaaaggaccg cggccggcgt gcgcttctgc gtcgacaaca
      961 cgggaccgcc gctcgcgaga gcggagatcg aggccatgtt ccagccgttc tggcaggcca
     1021 cgcacgaccg tcgcggcgcg ggcctcggcc tgtcgatctg ccggtccatc gtggaggcgc
     1081 acgggggcag catctggccc gagccggtgc agggcgagcg cgtccgcgtc tgcttcgtgc
     1141 tgccgcggca ccctttgacg gggcgcatcg ctcccccgcc tctcccgggg ccctgatact
     1201 tctggcgttc gccgggcgcg cttggcatca ttacgcgcac ccagggagcc cggcatgtac
     1261 gagcacagga cccagccgct gctgtcgcgc cgcaagttct tcggccggct ggcgcagagc
     1321 gccgccgtcg ctgcgggcct cgccttcgtg tcgctcgcga tcggaatgac cggataccac
     1381 gtcttcgagc gcctcgactg gatcgacgcc tttctcaacg ccgccatgct gctcggcggc
     1441 atggggcccg tggacgcgcc gatgaccgcc gcgggcaagc tcttcgccgg gctgtacgcg
     1501 ctttattgcg gcctggtcgt gatcatcgcc acgggcgtca tcctcgcgcc gctcgcgcac
     1561 cggctgatgc accggttcca cctggagcaa gagcgcgatc gcgaccattg atggacttcg
     1621 cgctcaccgg cgagcagcag cgcatccgcg acgcggtcgc gcgcctgtgc acgccgttcg
     1681 acgcgcaata ctggctagcg agggaccgcg acggcgcctt cccgcacgat ttccaccagg
     1741 ccttcgccga cgccggctgg ctcgggatcg cgatgcccga gcaatacggc ggcgcgggcc
     1801 tggggatcgt cgaggcggcg ctgatgatgc aggccgtgtc cgagtcgggc gcggggttct
     1861 cgggcgcgtc ggcgctgcac atgaacatct tcgggctgaa tcccgtcgtc gtgttcgcga
     1921 gcgaggagca gaagcgccgc tggctgccgc cgctcgtcgc gggccgggac cgcgcgtgct
     1981 tcgccgtcac cgagcccgac gccgggctga acacgctcgg gctgaagacg aaggcgctgc
     2041 gcgacggcga ccactacgtc gtctccggac agaagatgtg gatctccacc gcgcaggttg
     2101 cgacgaagat cctgctgctc gcgcgcacca cgcccgtcga agaagcccgg ggcacgcacg
     2161 gcctcacgct cttctacacc gacttcgacc gcgggaagat cgaagcgcgc gtcatcgaca
     2221 agatgggccg ccacgccgtc gactccaacc aggtcttcat cgacggcctg cgcgttcccg
     2281 cggccgaccg catcggcgac gacggccgcg gcttcgagta catcctgcac ggcatgaacc
     2341 ccgagcgcat cctgatcgcc gccgaggccg tcggcctcgg gcgcgccgcg ctcgcgcgcg
     2401 cggccgcgta cgcgaacgag cgcattgtct tcgaccgccc gatcgggcag aaccaggccg
     2461 tgcagcaccc gctcgcacgc tcctggatgg agctcgaggc cgcgaacctg atgaccttca
     2521 acgccgcgtc cctctacgac gccggcaagc cgtgcggcgc cgaggcgaac gcggcgaagt
     2581 acctcgccgc cgaagcctgc tggcgcgcgt gcgagaccgc cgtgctcacc cacggcggca
     2641 tgggctacgc gaaggagtac cacgtcgagc gctatctgcg cgaggcgtgg atcccgcgat
     2701 tggcgccggt cagcccgcag ctgatcctgt gcttcatcgc ggagaaggtg ctcgggttgc
     2761 cgaagtcgta ctgaagcgct gtcgcttcgc ccgagcctct cggccagcgc gaacaggttc
     2821 cgcgcctgcg aggcgctcgt gaacaccgtc gctccgacct cgccgcgctc gagcgcgtgg
     2881 agcagcgcgg cgagcggggc cgtgtccgcg ggcagcgacc agcggtaggt cgcgatctcg
     2941 accacctcgg cgcctttcgc ctccaatgcg tggtcgagct cctcgttcga cgcgccgtag
     3001 cgctgcacca gcgcacgctc gccccgcagc ggcaggtcgg cgatcgcggc gagcacctcg
     3061 gcggtggtgt acggctcggc ggccgagcgg tcgatgcgca cgccccgcgc gcgcagcgcc
     3121 gcggtgggct tcggcccgcg cgcggcgacg acggccttcg cgagcgcgtc gagcagcttc
     3181 ggcgtcgcgc cgagcgcgtc ggtcgcctcg aacagcgcgc gcgtcccgac gccggtctgg
     3241 aacacggcca atttcggcgg gcgcacaccg agcccgttca cgaggcgccc gatcgcatcg
     3301 aggtcgacgt cgggcgtctc ggcgagcgcg ggcgcggcga tgacgcgcgc gccgcgccgg
     3361 gcgatcagcc cggcgagctg cgtcgacagc cggctctcga ggatcgcgac cgtacgggat
     3421 ttcattgcgt cgattgtagc gtgcgccccg gagattaaac tccggctgaa acccgttcat
     3481 gcatcggcga tccaaaccca atgccgaccg tcctcatcac cggcgcaggc cgcgggctcg
     3541 ggctcgagtt cgcgcggcag tacgcggccg acggctggaa ggtcatcgcg accatccgcc
     3601 gatccgacgc caaggcgacg ctgcagtcgc tcggcgcgga cgtcgacggc ggcgtcgcgc
     3661 tcgacgtgct catcaacaac gccggcatct acggtccgag cggcctcgcg atcggcacgc
     3721 tcgactacgc cgcgtgggag caggtgctgc gcgtgaacgt gctcggaccg gccgcgatgt
     3781 ccgaggcgct ccgcgacaac gtcgcggcga gcgggcgccg catcatcgtc atgatctcga
     3841 gccgcctcgg ctcgatcatg gagatgcgcg ccggcgacta tctctacggc tcgagcaagg
     3901 ccgcgctgaa cgccgtcacc aagtcgttcg cctcggcgct cggacgcggc gtgatcgtcg
     3961 tcgcgctcag cccgggctgg gtgcgcaccg acatgggggg cgccggcgcg ccgctcgcgc
     4021 ccgagacgtc ggtcgccggg atgcggaaag tgatcgcggg actgtcgcgc cgggattccg
     4081 gccgcttcat caactacgac ggctcggaca ttccctggta gcgctcagga ggcctgctgc
     4141 ttgccgctct tggcggcctt ggcgccgggc tttcgctgcc actcctcgcg gcgaaagcgc
     4201 ttgcgacgcg ccggcactgg cttctgctcg gcgtactcct tcagccgcgc cgcgatgaaa
     4261 tcgcggatat cgtcgggcag cgtgttcgcc gccgactcga agcgcgcgac gatcgggtcg
     4321 tcggtgagcg gcgcgtcggg atgcaggcgc gcgcccagca cctgctccga cgagcgcgcc
     4381 gaatgcaggc cgtcgtggaa aaagccgccg tactggtgcc acaccagcgc gcgctcgctg
     4441 gagtcgatgc cgtagcacag ctggaacagc gtgatctcac cctcgcccgt ctgccagacg
     4501 aagagatcga aataatcgtc gtggaaccag cgccgcagcg tccggggctc gtcggtgacg
     4561 ccgagaattt ccttcatcat cgtattctgc ctcgccggcg gcgtttgcgc agccgtggtc
     4621 cgcgattaga ccttcccccg accggattgg accggaacgc cggttgctgg ttccttcggg
     4681 cggggccgtg cgcccgtcca acgccttgac gcgaaacgcg ttgcacgcgg ctgcaactcc
     4741 ccggccgggt cgcaatcgaa agggagacac cggggattcc cgaccccgac taatccggaa
     4801 agactatccc tcatgacgaa tgccctttcc cgccttgtag ccgtgctgct gatctcggtg
     4861 ctcgccggaa cgctgaacgg aaaagcggcg ctcgcccagc agcagagccc cgaccagctc
     4921 gtgaaggcgg cgaccgagga cgtgctcgcg gcgatcaaga agaccaccga ccgcgcgacg
     4981 ctgcgcaagg tcgcgcagca gaaggtgctt ccctacttcg acttcaccga gatgacgcgc
     5041 ctcgccgtcg gccgcccgtg gcgcgaagcg acgcccgagc agaagaagaa gctgcaggcc
     5101 gagttcaccg acctgctcgt caacacctac acctcggcgc tgacgcgcac cgtcgccgcc
     5161 gacaagaccg tggaggtgaa gccgctgcag gccggcgcga acgacaagga agtgacggtc
     5221 cgctcgctcg tcaagggcgg cggcgggcag ccgctcgaca tcgattaccg gatgacgaag
     5281 gaggaccagg gctggaaggt gatcgacgtg atcgtcgcca acgtcagcct cgtcaccacc
     5341 taccgcggca gcttcgcgca ggaggtgcag cagggcggca tcgacgggct gatcaagtcg
     5401 ctcgagggca agaaccgcca gctcgcgaag ggctaggcat tcccggccgc gatcggcctc
     5461 agtcgatcgt gatgtggcgc tcgcggatca gccgctccca gcgcgccgac tcggcgcgta
     5521 cgaacgaccc gaactccgcg ggcgagctcg cgaccacctc gtagccgaag ctctcgagct
     5581 tctccttcga aggtccggcc gcgagcgcct tgcggatctc gccgctcagg cggtcgacga
     5641 tcgccgccgg cgtgcccgcg ggcgcgagca agcccatcca cgactgcgtc tcgaacccgg
     5701 gataggtctc ggcgagcgtg gggacgtcgg ggagttgcgg cgcacgccgc ggcgaggtca
     5761 ccgcgatcgc caccagcttg cccgcgcgca cgtgctcgat cgcgacgccg agcgtgatga
     5821 tcatcatgtc cacctggccc gcgatcagcg caacggccgc gggcccgccg cccttgtagt
     5881 ggatcgccgt gggatcgatg cccgtgaggt ccttgaacag ctcgacgctc agccggctcg
     5941 aggtgccc
//
LOCUS       DATDMW010000042        24426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2002294, whole genome shotgun sequence.
ACCESSION   DATDMW010000042 DATDMW010000000
VERSION     DATDMW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24426
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002294"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VI319_01675"
     CDS             <1..232
                     /locus_tag="VI319_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862582.1"
                     /translation="ITPSGTVPPATTVTFNALGGVVPQNADGSLPFTQVDLSNPMVTG
                     PTARPLRVVVSAGGSIRMCDPAVAAPDPRACP"
     gene            complement(233..1177)
                     /gene="ispH"
                     /locus_tag="VI319_01680"
                     /gene_synonym="lytB"
     CDS             complement(233..1177)
                     /gene="ispH"
                     /locus_tag="VI319_01680"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY6862583.1"
                     /translation="MEVVLANPRGFCAGVERAIAIVERAIALHGAPIYVRHEVVHNQY
                     VVDDLRRKGAVFVEELSEVPEGSTVIFSAHGVSKAVQREAEARGLRVFDATCPLVTKV
                     HVEVTKMREAGREIVMIGHRGHPEVEGTMGQSHGGMYLVENVADVAGLEVSDPQKLSY
                     VTQTTLSVDDAAAIVQALKKRFPAITGPRKDDICYATQNRQDAVKFMAPKCDLVIVVG
                     SPNSTNSNRLREVAINVGTETYMIDNATQLRAEWLAGKRRVGVTAGASAPEILVKDVV
                     GRLRDLGAERVTQLEGATETVVFTLPRNLATRPDNGPR"
     gene            complement(1182..1757)
                     /gene="lspA"
                     /locus_tag="VI319_01685"
     CDS             complement(1182..1757)
                     /gene="lspA"
                     /locus_tag="VI319_01685"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY6862584.1"
                     /translation="MARIPRSAAGASPISSAAASRASMPSAGSAVFRSREATWWIVAA
                     VVVLADQATKWLVGELLASRPPAVITPFFNFVLAYNTGAAFSFLAEGSGWQRWLFAGI
                     AVVAAALISVLIRRHAEQRLFCAGLSLILGGAIGNLIDRLARGHVVDFLQLHAGDWYW
                     PAFNVADSAITVGAVLVVLDSLRSKPGQGLQ"
     gene            complement(1681..4500)
                     /gene="ileS"
                     /locus_tag="VI319_01690"
     CDS             complement(1681..4500)
                     /gene="ileS"
                     /locus_tag="VI319_01690"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY6862585.1"
                     /translation="MSEKKEDYRATLNLPDTPFPMRGDLARREPQWVKEWHERRVYQK
                     VRAASRGRPKFVLHDGPPYANGDIHIGTAMNKVLKDIIVKSRNMAGFDAVYVPGWDCH
                     GMPIEVQIEKTYGKNIPVEETQRRARAYAAEQIERQKKDFQRLGVLADWEHPYLTMDF
                     ANEAAEIRTLGKLLAKGYVYRGLKPVNWCFDCGSALAEAEVEYMDRRDFAIDAGFPFA
                     EPEKIAKAFGLLELPTTRGWIVIWTTTPWTLPGNQALNVHPEFTYELVDTSAGLLILA
                     AELREACLARYGLEGRPIASCKGTALERIAFRHPFYDRLAPVYLGDYVTLEQGTGIVH
                     SAPAYGVEDFVSCRRYGMKDEEILTPVLGDGTYAPSLPLFGGMSIWKANPKIVDKLRE
                     AGALFNAEEQSHSYMHCWRHKTPVIYRATTQWFAGMDDVPGYEGVKPAESLRATALRA
                     VDATRFYPSWGQARLHGMIANRPDWTLSRQRQWGVPMPFFIERETGALHPRTLELLEA
                     VAKKVEQGGIEAWQRVEPAELLGADAARYEKVKDTLDVWFDSGSTHETVLRGSHAAEL
                     KFPADLYLEGSDQHRGWFHSSLLVSCMLNARAPYDALLTHGFVVDGQGRKMSKSLGNV
                     VAPQKVSETLGAEILRLWVASTDYSGELSISDEILKRVVESYRRIRNTLRFLLANLSD
                     FDPERDALPPAQWLEIDRYAVALAADVQAGILADYERYEFHPAVARLQTFCSEDLGAF
                     YLDILKDRLYTTRADSRSRRSAQNALYHVTQGLLRLMAPILSFTAEEAWRVLAPGDET
                     IFVHTWYAFPQVPDGAALLAKWARIRVFRSEVQKLLEALRGTGEIGSSLQAEVDIAVN
                     GEESELLRSLDDDLRFVLITSRATVKPAASAGEARIVVAPSAHPKCARCWHYRADVGA
                     DRAHPALCGRCVANLFGSGEPREHA"
     gene            complement(4493..5428)
                     /locus_tag="VI319_01695"
     CDS             complement(4493..5428)
                     /locus_tag="VI319_01695"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY6862586.1"
                     /translation="MLLTHGAPRAALPAVAATIGNFDGVHLGHQAMLGRLDAAARARR
                     LASCVLTFEPHPREYFSPASAPTRLSSLREKCALLDSHGVERVHVLRFNRALAGLAPD
                     AFVREVLVRALKARWVLVGEDFRFGSRRAGDAAQLRRLAEAQGVEVETMPDVRHGGLR
                     VSSSAVRRALELGDLAGAEALLGRPYGISGRVVHGDKLGRALGFATANVQLRHNRPPL
                     TGIYAVRVHGVADAALPGVASLGVRPTVTASGKAVLEVHVFDFSADLYGRHLRVDFLH
                     KIRDEEKYPDLETLRAQIARDCDAARALLTESRHV"
     gene            5510..6022
                     /locus_tag="VI319_01700"
     CDS             5510..6022
                     /locus_tag="VI319_01700"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEY6862587.1"
                     /translation="MDALARQMSVYAAYHRNRWNRATHFIGVPAIVFAIFIPLHWVAL
                     GGGATLAHALAAAVLLYYVILDVAIGLATAAVVLALLEAASVAGSSGTAAGWSWFAAF
                     FVGGWIFQLVGHVFEGRRPALVDNVLQVFVAPVFLVAEVFFMLGLKRALRERMEGPAG
                     ARAARAPGVN"
     gene            complement(6015..6848)
                     /locus_tag="VI319_01705"
     CDS             complement(6015..6848)
                     /locus_tag="VI319_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6862588.1"
                     /translation="MTPQLERFAEIVSREQFDLAEASLLLAQDAYPGMDVGGYLGRLD
                     DIAGAIRRRLAGDAFAEQKVLALNYYLFNELRYCGNIDDYYDPRNSYLNEVMERRTGI
                     PITLSIVYLEIGRRIGLNLKGVSFPGHFLVKLSVKRGELVLDPFIGGEAQSEADLRQR
                     LAQVLPAAQAERAKLEQYLEPATPRQIIARVLRNLKNIYTQTRKLEAALAVMHRMLLV
                     VPESAEELRDRGMLYQQLECFRPALSDLQNYLRRRPDAPDATEIHGKIVELRQASAKL
                     N"
     gene            complement(6845..8386)
                     /gene="murJ"
                     /locus_tag="VI319_01710"
     CDS             complement(6845..8386)
                     /gene="murJ"
                     /locus_tag="VI319_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY6862589.1"
                     /translation="MNLLRAAATVSAMTFISRVTGLIRDMLIAAMFAASPATDAFVVA
                     FRIPNLLRRLFAEGAFSQAFVPLLAEYKNRRGEEETRRLIDRTATTLALALLAVTAIG
                     VVISPLLIYLSAPGFSGDPAKFDLTVRMLRITFPYILFISLVSFASSILNTWSRFAVP
                     AFTPTLLNLSFIAGALLFAPYFHPPIVVLAWSVFAGGVLQLAFQITGLLRLRLLPRPA
                     FTWNDPGVRRILVLMGPAVIGVSVGQLSLLINTIFASFLENGSVTWLYYADRLMEFPT
                     ALLGVALGTVLLPSLAKHHADANTAEYSRLLDWGLRLTFMLAAPAALALAILSVPLIT
                     TLFYHGIFTEQHVFMTRRALVAYAVGLLGLILVKVLAPGFYARQNIATPVRIAILTLF
                     VTQVMNAAFIVPLKHAGLALSIGLAACLNAGLLYRQLRQHGIYAPQPGWSTFGLKVSA
                     ALMAMGAVLWLAMGPESWWLAAGSASRAAALAGLVLAGVVVYFGALWALGFRARDFVL
                     RAPGT"
     gene            8489..8791
                     /gene="rpsT"
                     /locus_tag="VI319_01715"
     CDS             8489..8791
                     /gene="rpsT"
                     /locus_tag="VI319_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY6862590.1"
                     /translation="MATTAGTKTAKKSARTKGARKAARQAEKRRKHNAGQRSALRTAL
                     KNVVKAIGAGDKAAAQKAFREAASVVDRVADKNIIHKNKAARHKSRLAARIRALAG"
     gene            8855..10825
                     /locus_tag="VI319_01720"
     CDS             8855..10825
                     /locus_tag="VI319_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6862591.1"
                     /translation="MKKPVSRVLIINDEPLVLKEFVKGLNAAARSLDNPVGITFQGVT
                     TAREALAVIERDGDLQAVVVDDKLYTLKNPSTKKEQASALELVQRITRFRPELDVYIL
                     IAQEQEDEIVDTLFAEAVDGYFYREERDYRGMYRILNAQIQERSRTPFYDQLKNYVWM
                     AKDSWHTPGHSSGESLRGSPWVNDFYDFMGEHVFDADLSVSVKMLDSLMEPTGVIAEA
                     QTIAAKAFGARRTFFATNGTSTANKVIFQTLLAPGEKLLLDRNCHKSVHHGVVLSGAH
                     PIYLDSSVNAKYSLYGPVPKKTILAAIRKHPDAQALILTSCTYDGLRYDLKPIVEAAH
                     AKGIKVIVDEAWYGFARFHPSFRPTALEAGADYATQSTHKVLSAFSQASMIHVNDPAF
                     NEHLFRENFNMHTSTSPQYSMIASLDVARKQAVMEGYKLLSRTLMLSQELRGQINSTG
                     VFRVLELEELLPEKVRSDGIRLDPTKVTVDITSCGYTVEDLQRQLFDRYNIQVEKSTF
                     NTVTLLLTIGTTRSKVSRLYDALMRIAREGRAPRRLYRFPEIPGFTRLRYLPRDAFYC
                     GGELVPLVDERDRVRRELLNRVCADQIVPYPPGIPVLVPGQVITRDIVTYLVGLLRSQ
                     NRVELHGIVYDGYLPCLRVLKPAEEKGLQALA"
     gene            10903..11148
                     /locus_tag="VI319_01725"
     CDS             10903..11148
                     /locus_tag="VI319_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862592.1"
                     /translation="MSLSSTTTKYGFRIRTRDGMVVEHLMIHGRDEADAERKLRQMYL
                     HCEILECSIMQPAMMQPAKRSSAAAASFEDVISLISK"
     gene            complement(11172..11936)
                     /locus_tag="VI319_01730"
     CDS             complement(11172..11936)
                     /locus_tag="VI319_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY6862593.1"
                     /translation="MGTRLTVFNQKGGVGKTTTTLNLGAALARIGLLPVLVDLDAQAH
                     LTSILNPISSSKESLFTLYSESKPLAGIARPVAFGGQLLPAHTELIKIDSMFGKGPDV
                     LNRLKQGLDANYESSQPVVIDCSPMLGVLSLSAVFATERVLVPVSADYLAMKGAVALE
                     KTLKALEHVLKQRVERRYVITRFDSRRRMAHDIAAQLGERFGAELCETRIAESVGIAE
                     SPAFGKDVFSHDPDGRGAQDYARLLEELRSSGFWNV"
     gene            12175..13377
                     /locus_tag="VI319_01735"
     CDS             12175..13377
                     /locus_tag="VI319_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY6862594.1"
                     /translation="MSHVMNTYSRLPVSFTRGEGVWVWDDAGRRYLDALAGIAVNTLG
                     HAHPGLVRVIAEQAARLIHVSNLYGIAEQEKLADKLAAISGMDNVFFCNSGCEANEAA
                     IKIARLYGHKKGIESPAIVVMDKAFHGRTLATLSATGNRKVQKGFEPLVPGFVRVPFN
                     DIEAVRRVAAADRNIVAVLVEPIQGEGGINISSLQYLRELRELCDRQGWLLMVDEVQC
                     GLGRTGRWFVYQHAGVLPDVMPLAKGLGSGLPIGACLARGAAARVFEPGNHGSTFGGN
                     PLACAAAFATLSIVEEEGLLENAARMGALLLSGLREQLAGVPGVADVRGHGLMIGIEL
                     DRPCGVLVGRALEQALLINVTMDNVVRLLPALVISEAEVRQLLSGLVPLIRGFLAEPA
                     AAASAARA"
     gene            13379..14314
                     /gene="argF"
                     /locus_tag="VI319_01740"
     CDS             13379..14314
                     /gene="argF"
                     /locus_tag="VI319_01740"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY6862595.1"
                     /translation="MPAEHKPRHYLEFRDLQREEYEHVYARTRWIKDRFKRYERYWPL
                     EDRTLAMIFEKPSTRTRMSFEAGMHQLGGAAIYLYTRDSQLGRGEPVEDAAQVISRMC
                     DLIMVRTFEQDIIERFAANSRVPVINGLTNEYHPCQVMADIYTYIEERGPIRGKTVAW
                     IGDSNNVCNTWLQAATILDFNVHVSTPPGYEVEPERAGIYDDSHYESFSDPMDAARGA
                     DLVTTDVWTSMGFEAENEERKRDFQDWQVDADMMRAAKRDALFMHCLPAHRGEEVAAE
                     VIDGPQSVVWDEAENRLHTQKALMEFLLLGKIERT"
     gene            14325..15554
                     /locus_tag="VI319_01745"
     CDS             14325..15554
                     /locus_tag="VI319_01745"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY6862596.1"
                     /translation="MKGKEDGIRKVVLAYSGGLDTSVILKWLQDVYRCEVVTFTADIG
                     QGEELEPARAKAKALGVKEIFIDDLREEFARDFVFPMFRANTIYEGEYLLGTSIARPL
                     IAKRQIEIARKTGADAVAHGATGKGNDQVRFELGYYALEPGIHVIAPWREWDLTSRET
                     LLAYAEKHGIPIEGKKKGGSPYSMDANLLHISYEGRILEDPSREPEEDMWRWTVSPEK
                     APDRPQVVELEYRCGDIVAVDGKTLTPGAALARLNELGGQHGIGRLDLVENRYVGMKS
                     RGCYETPGGTIMLKAHRAIESLCLDREVAHLKDELMPRYASLIYNGYWWSPERRMLQT
                     MIDASQASVNGKVRVKLYKGNVMVVGRQSDDSLFDPAIATFEDDRGAYNQADAAGFIR
                     LNALRMRIAAKLKTKRR"
     gene            15637..16368
                     /locus_tag="VI319_01750"
     CDS             15637..16368
                     /locus_tag="VI319_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY6862597.1"
                     /translation="MRILVLSDIHLEVAPFEPAASSADLVVLAGDIANGGAGVDWAER
                     SFRAPVLYVPGNHEYYDHEFDRANESLRKAAGARVRLLDCKEAITDDVRFLGCTLWTD
                     YTLVPDAARPAVVEEARAKNPDYAKIRFDGRNFTPEHAMALCRKHRAWLERKLYEPFG
                     GRTVVITHFAPHPNSIAPAYMGHPANPGFVLDLSPLMGRAALWIHGHTHTAFDYEVKG
                     TRVVCNPRGYPGEPTGFRADLIVEI"
     gene            complement(16331..16789)
                     /locus_tag="VI319_01755"
     CDS             complement(16331..16789)
                     /locus_tag="VI319_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEY6862598.1"
                     /translation="MRPTRSRSWFSRFAKWTARTAGRPSAFAVAAAVIVVWGVTGPVF
                     GFSDTWQLVINTGTTIVTFLMVFLIQNTQNRDSEAIQVKLDEIIRSINGAHTALLDLE
                     ELEEEELDAIHREYCRLAERARRDLRHGKYDTGTPEIRVKSPRSGRRGSP"
     gene            16843..17328
                     /locus_tag="VI319_01760"
     CDS             16843..17328
                     /locus_tag="VI319_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY6862599.1"
                     /translation="MPSFDIVSEVNKVELRNAVDQANKEIANRFDFRGSDARIEQNEL
                     ALTFYADDDFKLKQVAELFMTKCAKRGVDVRAFDPQGIEKIGGDKMKQPVNVRSGIEQ
                     ERAKQIVKLVKDSKLKVQASIQGAAVRVSGAKKDDLQTVIQFLRKQVTDIPVQFNNFR
                     D"
     gene            complement(17329..18030)
                     /locus_tag="VI319_01765"
     CDS             complement(17329..18030)
                     /locus_tag="VI319_01765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY6862600.1"
                     /translation="MKPVAIFRHFGTEGPGYFATYLDDHRIPWRLVKIDAGEPVPADP
                     GPYSGLVFMGGPMSVNDELPWIPRVLALIDAAVRANIPVLGHCLGGQLISRALGGRVE
                     RNPVKEIGWGRVTVEANPQAREWFGDLKQFDSFHWHGETFSIPPGAVRLAASPYCVNQ
                     AFALGNHLGMQCHVEMTPEMIRTWCRGWKQEGIVGRAPSVQTPAEMQMDLGRRVDALN
                     GIARRVYDRWIRGLA"
     gene            18041..18688
                     /locus_tag="VI319_01770"
     CDS             18041..18688
                     /locus_tag="VI319_01770"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY6862601.1"
                     /translation="MNRMRHSIACSALFLCAAPALATDVNVVGLFPGKAVVSINGGQP
                     RTLHEGQKTDEGVTLLGTERDQATLEIDGRKRVLKMGQVYVAAPGGSSQQTVTLAADS
                     RGHFFVNGQVNGGQMRFLVDTGATWVALSTADAVRLAIDYRKGEPNLLSTANGVAAAY
                     KLKLDTVRVGDITISNVDAVVMDGASPGVALLGMSFLNRMQMRRDGGTMVLTKRF"
     gene            complement(18685..19497)
                     /gene="folE2"
                     /locus_tag="VI319_01775"
     CDS             complement(18685..19497)
                     /gene="folE2"
                     /locus_tag="VI319_01775"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY6862602.1"
                     /translation="MNTRDPLAIPDVQSAEDKRKLAIDKVGIKGIRHPVRIAERGGGT
                     QHTVAMFNMYVGLPHQFKGTHMSRFVEILNAHEREISVDTFKLMLGEMVQRLEAESGH
                     IEMRFPYFVSKAAPVSGVQSLMDYDVTFTGEVEKGSKCFKMTVVVPVTSLCPCSKRIS
                     EYGAHNQRSHVTISARTRGFVWIEELVEIAERNASSELYGLLKRPDEKHVTERAYDNP
                     KFVEDLVRDVAAELNRDDRIDWYVVESENFESIHNHSAYARIEKDKREKPAR"
     gene            complement(19614..21527)
                     /gene="dxs"
                     /locus_tag="VI319_01780"
     CDS             complement(19614..21527)
                     /gene="dxs"
                     /locus_tag="VI319_01780"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY6862603.1"
                     /translation="MSAIGEADLPGDGGVAGSCGYDPKRYELLKTINDPADLRKLERR
                     QLAQLADELREFVLQSVSQTGGHLSSNLGTVELTIALHYVFDTPYDRIVWDVGHQTYP
                     HKILTGRREAMSRLRMLGGISGFPRRGESPYDTFGTAHSSTSISAALGMAQAAKLAGE
                     PRHCIAVIGDGAMSAGMAFEALNNAGVMETDLLVILNDNDMSISPAVGALNRYLARLM
                     SGRFYAAAKRGAEKVLAGVPSLRELARKVEEHAKGLVVPSTLFEEFGFNYVGPIDGHD
                     LDSLVPTLKNIKELKGPQFLHVVTKKGQGYKMAEADPVLYHGPGKFDPAEGIKKASGG
                     KVTFTQVFGDWLCDMAVADRRLVGITPAMREGSGLVRFAKEHPDRYFDVGIAEQHAVT
                     FAAGLACEGLKPVVAIYSTFLQRGYDQLIHDVCIQNLPIVFALDRAGLVGGDGATHNG
                     AFDLSYLRCLPNMVVMTPADENECRRMLTTAFRLDQPSAVRYPRGSGPGVAVRPELEP
                     LPFGEGEVRRKGRRVAILAFGTLLKPALEAGDALDATVVNMRFVKPLDADLAARMARE
                     HELIVTVEENAVMGGAGSAVAEALSAAAIATPVLRLGLPDRFVDHGDQGQLLATVGLD
                     AGGIAAAVRARLA"
     gene            complement(21611..22489)
                     /locus_tag="VI319_01785"
     CDS             complement(21611..22489)
                     /locus_tag="VI319_01785"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY6862604.1"
                     /translation="MAWMGQVQGRVEAALGRFLPSGEIVPQRLHQAMRYAVLGGGKRV
                     RPLLAFAAGELAGADPSRLEVVACAVECIHSYSLVHDDLPCMDDDVLRRGKPTCHVEY
                     DEATALLVGDSLQSLAFQLLAEHRLADDPGVQLEMLRVLAAASGSRGMAGGQAIDLAS
                     IGRSLTLPELEHMHVHKTGALIRASVVLGARCGRPLPEEQLRELERYAKCIGLAFQVV
                     DDVLDREGSTATLGKTAGKDAAHNKPTYVSVLGTARARALAEELRAEAAAALRPFGAA
                     ARRLADLADFVVLREF"
     gene            complement(22506..22766)
                     /locus_tag="VI319_01790"
     CDS             complement(22506..22766)
                     /locus_tag="VI319_01790"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY6862605.1"
                     /translation="MIPEPAGPMSKPAETPASFEAALSELEKLVASMESGKLTLEQSL
                     SAHKRGLELAQYCQSVLSRAQQQVKVLEENTLKTLPGEPPEH"
     gene            22939..24042
                     /locus_tag="VI319_01795"
     CDS             22939..24042
                     /locus_tag="VI319_01795"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY6862606.1"
                     /translation="MSEIATTSKLRPSTAQLPVSWYFDSRIFDLEQRLLFETGPKYVG
                     HELMVPNPGDYHTLSWMDHGKALVRNTNGIELLSNVCRHRQSILLEGRGNAQNIVCPL
                     HRWTYDLKGELLGAPHFADNPCLNLPRTRLQSWQGMLFEGGRDVARDLANFSLAPDFD
                     FSGYLFDHVEVMELEQNWKTFIEVYLEDYHVVPYHPGLGGFVTCDDLRWEIADTYSAQ
                     VVGVNNGLKQPGTPAYAKWHEACTKYLDGRAPKQGAIWMLYYPNVMLEWYPHVLVVST
                     LLPRSPTLTTNVVEFYYPEDIVLFERAFIEAERAAYMETAIEDDEIAKRMDRGRRALM
                     LRGEDDAGPYQSPMEDGMMHFHEYIRRGLAPGL"
     gene            24077..>24426
                     /locus_tag="VI319_01800"
     CDS             24077..>24426
                     /locus_tag="VI319_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6862607.1"
                     /translation="MSYTTLVSTGVLARHLDDPAWVVFDCRHDLADPARGRREYARSH
                     IPGARFLHLDDDLSSPCTGTNGRHPLPDFAALGRKLAGAGVAPGVQVVGYDAQGGMVA
                     ARLWWLLRGLGHDQV"
BASE COUNT         4062 a         8367 c         8158 g         3839 t
ORIGIN      
        1 cattaccccg tcgggaaccg tccctcccgc gacgacagtc actttcaacg cgctcggcgg
       61 agtggtgcct cagaatgcgg acggatcctt accgttcacg caggtcgacc tcagcaatcc
      121 gatggtgacg ggccccaccg cgcgtccgct gcgcgtggtc gtatccgcgg gcggcagcat
      181 acggatgtgc gatccggccg tcgccgctcc ggatccgcgc gcctgtccct gatcagcgcg
      241 gcccgttatc gggccgcgtg gccagattcc tcggcagcgt aaacacgacc gtctcggtcg
      301 cgccttcgag ctgagtgacg cgctcggcgc ccaggtcgcg caagcgcccg accacgtcct
      361 tcaccaggat ttcgggtgcc gacgcgccgg cggtcacacc cacccgccgc ttgcccgcca
      421 gccattcggc gcgtaattgg gtggcgttat cgatcatgta ggtctcggtg ccgacgttga
      481 tggcgacctc gcgcaggcgg ttggagtttg tgctgttggg cgagccgacg acgatcacga
      541 gatcgcactt cggcgccatg aacttgaccg cgtcctgccg gttctgggtc gcgtagcaga
      601 tatcatcctt cctcggcccc gtgatcgcgg ggaagcgctt cttcagcgcc tggacgatcg
      661 cggccgcgtc gtcgaccgag agcgtggtct gggtcacgta ggaaagcttc tgcgggtcgc
      721 tgacctcgag cccggccacg tcggcgacgt tttccaccag gtacattccg ccgtgggact
      781 gccccatcgt gccttcgacc tccgggtgcc cgcgatggcc gatcatgacg atctcgcgac
      841 cggcctcgcg catcttcgtg acctcgacgt gcactttggt cacgagcgga cacgtcgcat
      901 cgaagacgcg cagcccgcga gcttccgcct cgcgctggac cgccttggac acgccgtggg
      961 cgctgaagat caccgtgctg ccttcgggca cctcggacag ctcctcgacg aacaccgcgc
     1021 ccttgcggcg caggtcgtcg acgacgtatt ggttgtgcac cacctcgtgc cgcacgtaga
     1081 tcggcgcgcc atggagcgcg atcgcgcgct cgacgatggc gatcgcgcgc tccacgccgg
     1141 cacagaagcc cctgggattg gcgagaacga cttccatgcc gctattgtag tccctgcccc
     1201 ggcttgctcc ggaggctgtc gagcaccacg agcaccgccc cgaccgtgat ggcggagtcc
     1261 gccacgttga aagccggcca gtaccagtcg cccgcgtgca gctgcaggaa atcgaccacg
     1321 tgcccgcgcg ccaggcgatc gatgagattg ccgatcgctc cgccaaggat cagggacagg
     1381 ccggcgcaga agagcctctg ctcggcgtga cgccggatca gcaccgagat gagcgccgcg
     1441 gcgaccaccg cgatgccggc gaacagccat cgctgccagc ccgatccctc ggcgaggaag
     1501 ctgaacgcgg ctccggtgtt gtaggcgagc acgaagttga agaacggcgt gatcaccgcg
     1561 gggggccgcg atgccagcag ctcgcccacc agccacttgg tcgcctggtc ggcgaggacc
     1621 acgacagcgg cgacgatcca ccaggtcgcc tcccggctgc ggaagacggc gctgccggcg
     1681 ctaggcatgc tcgcgcggct cgccgctgcc gaagagattg gcgacgcacc ggccgcagag
     1741 cgcgggatgc gcgcgatcgg cgccgacgtc cgcgcggtag tgccagcagc gcgcgcactt
     1801 cgggtgcgcg ctcggcgcca cgacgattcg cgcttcgccg gcggacgcag ccggcttcac
     1861 ggtcgcacgc gacgtgatga gcacgaagcg caggtcatcg tcgaggctgc gcaggagctc
     1921 cgattcctcc ccattcaccg cgatatcgac ctcggcctgg agcgacgagc cgatttcgcc
     1981 cgtcccgcgc agcgcctcga gcagcttctg cacttccgag cggaacacgc ggatgcgcgc
     2041 ccacttcgcg agcagggccg cgccgtcggg cacctgcggg aacgcgtacc aggtatgcac
     2101 gaagatcgtc tcgtccccgg gggctagcac ccgccaggcc tcctcggccg tgaacgacag
     2161 gatcggcgcc atcaggcgca gcaggccctg cgtgacgtgg tacagcgcgt tctgcgccga
     2221 tcgccgcgag cgcgagtcgg cgcgcgtggt atagagcctg tccttcagga tgtcgagata
     2281 gaacgcgccc aggtcctccg agcagaacgt ctgcagccgc gccaccgccg ggtggaactc
     2341 gtagcgctcg tagtccgcga ggatcccggc ctggacgtcg gccgcgagcg ccaccgcgta
     2401 gcggtcgatc tcgagccact gcgcgggcgg gagcgcgtcg cgctccgggt cgaagtccga
     2461 gaggttggcg agaagaaaac gcagcgtgtt gcggatgcgg cggtacgatt ctacgacgcg
     2521 cttcaggatc tcgtccgaga tcgacagttc gcccgagtag tcggtcgagg cgacccagag
     2581 ccgcaggatc tcggcgccca gggtttcgga cactttttgc ggcgccacga cgttgcccag
     2641 cgatttcgac atcttgcggc cctggccgtc gacgacgaag ccgtgcgtga gcagcgcgtc
     2701 gtagggcgcg cgcgcgttca gcatgcagga gaccagcagc gacgaatgga accagccgcg
     2761 atgctggtcc gacccctcga ggtagagatc ggcggggaac tttagctccg ctgcgtgcga
     2821 gccgcgcagc acggtctcgt gcgtcgagcc cgaatcgaac cacacgtcga gcgtgtcctt
     2881 caccttctcg tagcgcgccg catcggcgcc cagcagctcg gccggctcga cgcgctgcca
     2941 ggcctcgatc ccgccctgct ccaccttttt cgcgaccgcc tccagcagct ccagcgttcg
     3001 cgggtgcagg gcgccggtct cgcgctcgat gaagaacggc atgggcacgc cccactgccg
     3061 ctggcgcgag agcgtccagt ccggacggtt ggcgatcatg ccgtgaagcc gcgcctggcc
     3121 ccatgacgga taaaagcgcg tagcatcgac cgcgcgcagc gcggtggcgc gcaggctctc
     3181 ggccggcttc acgccctcgt atcccggcac gtcgtccatc ccggcgaacc attgggtggt
     3241 ggcgcggtag atcaccggcg tcttgtggcg ccagcagtgc atgtagctgt gggactgctc
     3301 ctcggcgttg aacagggcac cggcctcgcg cagcttgtcg acgatcttcg ggttcgcctt
     3361 ccagatgctc atgccgccga agagcggcag cgacggcgcg tacgtgccgt cgccgagcac
     3421 gggcgtcagg atctcctcgt ccttcatccc gtagcgccgg cacgagacga agtcctcgac
     3481 gccgtacgcg ggcgcgctgt ggacgatgcc cgtgccctgc tcgagcgtca cgtagtcgcc
     3541 gaggtacacc ggcgcgaggc ggtcgtagaa cggatgccgg aacgcgatgc gctcgagggc
     3601 cgtgcctttg cagctcgcga tgggcctgcc ctcgaggcca tagcgggcga ggcacgcctc
     3661 gcgcagctcg gccgcgagga tcagaagccc ggcgctcgtg tccacgagct cgtaggtgaa
     3721 ctcgggatgg acgttcagcg cctggtttcc cggaagggtc cagggcgtgg tcgtccagat
     3781 cacgatccag ccccgggtcg tgggcagctc cagcagcccg aatgccttcg cgatcttctc
     3841 gggctcggcg aacggaaacc cggcgtcgat cgcgaagtcc ctgcggtcca tgtactcgac
     3901 ctcggcctcc gcgagcgccg agccgcaatc gaagcaccag ttgacgggct tcaggccgcg
     3961 gtacacgtag cccttcgcaa gcagcttgcc caacgtgcgg atctccgcgg cctcgttggc
     4021 gaagtccatg gtcaggtagg gatgctccca gtcggcgagc accccgagac gctgaaaatc
     4081 cttcttctgc cgctcgatct gctcggccgc gtacgcccgc gcgcggcgct gcgtctcctc
     4141 gaccgggatg ttcttgccgt aggtcttctc gatctgcacc tcgatcggca tgccgtggca
     4201 gtcccagccc ggcacgtaga ccgcgtcgaa gcccgccatg ttgcgcgact tgacgatgat
     4261 gtccttcagg accttgttca tcgccgtgcc gatgtggatg tcgccgttcg cgtacggcgg
     4321 gccgtcgtgc agcacgaact tcggccggcc gcggctcgcc gcgcgcacct tctggtagac
     4381 acggcgctcg tgccactcct tcacccactg cggctcgcgc ctggcgagat cgccgcgcat
     4441 cgggaacggc gtgtcgggca ggttcagcgt cgcccggtag tcctccttct tctcagacat
     4501 gccgggactc cgtgagcaac gccctcgccg cgtcgcagtc gcgcgcgatc tgcgcgcgca
     4561 gcgtctcgag gtcgggatat ttctcctcgt cgcggatctt gtgcagaaag tccacgcgca
     4621 gatgcctgcc gtagagatcg gcggaaaaat cgaacacgtg cacctccagg accgccttgc
     4681 cgctcgcggt gacggtcgga cgaacgccca ggctcgccac tcccggaagg gccgcgtcgg
     4741 ccacgccgtg cacgcgcacc gcgtagatcc cggtgagcgg cggacggttg tgccgcagct
     4801 gcacgttcgc ggtcgcgaag ccgagcgcgc gtcccagctt gtcgccgtgg accacgcgcc
     4861 cgctgatgcc ataggggcgg ccgagcagcg cctcggcgcc ggccaggtcg cccaactcga
     4921 gcgcccggcg cacggccgag ctcgacacgc gcagcccccc gtgcctcacg tcgggcatcg
     4981 tctccacctc cacgccctgg gcttcggcga gccggcgcag ctgcgccgcg tctccggcgc
     5041 gccgcgaccc gaagcgaaaa tcctccccga ccagcaccca gcgcgccttg agcgcccgca
     5101 cgaggacctc gcgcacgaac gcgtcggggg cgagccccgc cagggcgcga ttgaagcgca
     5161 gcacgtggac gcgctcgacg ccgtgcgagt cgagcagcgc gcacttctcg cgcagcgacg
     5221 agaggcgcgt cggagcgctc gcgggcgaga aatactcgcg cggatgcggc tcgaacgtca
     5281 gcacgcagct cgcgagccgc ctggcgcggg ccgccgcgtc gagccgcccg agcatcgcct
     5341 ggtgtccgag gtgcacgccg tcgaagttgc caatcgtggc cgcgaccgcg gggagcgcgg
     5401 cgcgtggagc gccgtgggtg agaagcatcg agggaccggg aaaagctgca aagttttgaa
     5461 ttatagcggc ttttccggtc gaccggaacg agcgtaacat cctgccgtca tggacgcgct
     5521 cgcccggcag atgtcggtct acgccgcgta ccaccgcaac cggtggaacc gcgcgacgca
     5581 tttcatcggc gtgccggcga tcgtgttcgc gatcttcata ccgctgcact gggtcgccct
     5641 cggcggcggc gcgacgcttg cgcacgcgct cgctgcagcg gtgcttctct actatgtgat
     5701 cctcgacgtc gccatcgggc tcgccacggc ggccgtcgtc ctggcgctgc tcgaggcggc
     5761 gtcggtcgcc ggatcgtcgg gaaccgcagc gggctggagc tggtttgccg cgttcttcgt
     5821 cggcggctgg atcttccagc tggtgggtca tgtcttcgaa gggcgccggc cggcgctcgt
     5881 cgacaacgtg ctgcaggtct tcgtcgcgcc ggtgttcctc gtggccgagg tgttcttcat
     5941 gctgggattg aagcgggctc ttcgcgaaag aatggaaggc ccggccggcg cacgcgccgc
     6001 gcgagccccg ggcgtcaatt gagtttcgcg ctcgcctgcc tcaactccac gatcttgccg
     6061 tggatctccg tcgcatcggg ggcgtcggga cgacgcctca ggtagttctg caggtccgac
     6121 agggccggcc ggaagcactc gagctgctgg tacagcatcc cccggtcgcg cagctcctcg
     6181 gcggactccg gcacgacgag cagcatgcgg tgcatgaccg ccagcgcggc ctcgagcttg
     6241 cgcgtctgcg tgtagatgtt cttgaggttg cgcagcaccc gggcgatgat ctggcggggc
     6301 gtcgcgggct ccaggtattg ctcgagcttc gcgcgctcgg cctgcgccgc gggcagcacc
     6361 tgcgccaggc gctgccgcag gtcggcctcg gactgcgctt ccccgccgat gaagggatcg
     6421 agcaccagct cgccacgctt caccgagagc ttcaccagga agtggcccgg aaacgacacg
     6481 cccttcaggt tcaggccgat gcgcctgccg atctcgaggt acacgatcga gagcgtgatc
     6541 gggattcccg tgcgccgctc catgacctcg ttgaggtagc tgtttcgggg atcgtaatag
     6601 tcgtcgatgt tcccgcagta gcgcagctcg ttgaacaggt agtaattgag cgccagcacc
     6661 ttctgctcgg cgaaggcgtc gccggcaagc cgtctgcgga tcgcacccgc gatatcgtcg
     6721 agccggccga ggtagccgcc gacgtccatg ccgggatacg cgtcctgcgc gagcagcagg
     6781 ctcgcctcgg ccaggtcgaa ctgctcgcgc gagacgatct cggcgaagcg ctccagctgc
     6841 ggcgtcatgt cccgggcgcg cgcaggacga aatcgcgggc gcggaagccg agcgcccaca
     6901 gcgcaccgaa ataaacgacg acgccggcga gcaccagtcc ggcgagcgcg gcggcccggc
     6961 tcgcgctgcc ggccgcgagc caccacgatt ccgggcccat cgcgagccac agcaccgcgc
     7021 ccatcgccat cagggccgcc gacaccttca gcccgaacgt gctccatccg ggctgcggcg
     7081 catagatgcc gtgctggcgc agctggcggt acagcaggcc cgcgttcagg cacgcggcga
     7141 gcccgatcga cagcgccagt ccggcgtgct tgagcgggac gatgaaggcc gcgttcatga
     7201 cttgcgtgac gaagagcgtg aggatcgcga tgcggaccgg cgtcgcgata ttctgccgcg
     7261 catagaaccc cggcgccagc accttcacga ggatcagccc gagcaggccg accgcgtaag
     7321 cgaccagcgc ccggcgcgtc atgaagacgt gctgctcggt gaatattccg tggtagaaca
     7381 gcgtggtgat caggggcacg gacaggatcg cgagcgcgag cgcggccggc gccgcgagca
     7441 tgaacgtcag gcgcaagccc caatcgagca gcctggaata ttcggccgtg ttcgcgtcgg
     7501 cgtggtgctt cgcgaggctg ggaagcagga cggtcccgag cgcaactccg aggagcgcgg
     7561 tcggaaactc catgagccga tcggcgtaat agagccacgt gacgctcccg ttctccagga
     7621 acgacgcgaa gatcgtgttg atcagcaggc tcagctggcc caccgatacg ccgatcaccg
     7681 ccggccccat caggaccagg atccgccgga caccgggatc gttccaggtg aacgccgggc
     7741 gcggcaggag acgcaggcgc agcagccccg tgatctggaa cgcgagctgg agcacgccgc
     7801 ccgcgaatac cgaccaggcg agcaccacga tcggcgggtg aaagtacggc gcgaacagca
     7861 gcgcgccggc gatgaagctc aggttgagca gggtcggcgt gaatgccggc accgcgaatc
     7921 ggctccaggt attcaggatg ctgctcgcga aggagacgag cgagatgaac aggatgtacg
     7981 ggaaggtgat gcgcagcatc ctgacggtca ggtcgaactt cgcggggtct cccgagaagc
     8041 cgggggcgct cagatagatc agcaacggcg agatcaccac cccgatcgcg gtgaccgcaa
     8101 ggagcgcgag cgccagcgtc gtcgccgtgc ggtcgatcag gcgccgggtt tcctcctcgc
     8161 cccgcctgtt cttgtactcg gcgagcagcg gcacgaaggc ctgcgagaag gccccctcgg
     8221 cgaacaggcg ccgcagcagg ttcgggatgc ggaatgcaac gacgaaggcg tctgtcgcgg
     8281 gactggccgc gaacatcgcg gcgatgagca tgtcgcgtat caggccggtg acccgcgaga
     8341 tgaacgtcat cgcgctcacc gtcgcggccg ccctgagcag attcatgcgc ggcattatag
     8401 ctttcgctat ccgtccgata ctcgccggcc ttgcgccagc cccttgcagc cattagaatt
     8461 tcgccctttc cccggagccg cacccagcat ggccaccacc gcaggaacga aaacggcgaa
     8521 gaaatccgcg cgcaccaagg gcgcgcgcaa ggcggcgcgc caggccgaga agcgccgcaa
     8581 gcacaacgcc gggcagcgct cggcgctccg caccgcgctg aagaatgtcg tgaaggcgat
     8641 cggcgccggc gacaaggcgg ccgctcagaa ggcgttccgc gaagccgcga gcgtcgtcga
     8701 tcgcgtcgcg gacaagaaca tcatccacaa gaacaaggcg gcccggcaca agagccggct
     8761 cgcggcgcgc atccgcgcgc tcgcgggcta gctctccacc gagcgcccga aaccgaagga
     8821 aggcggcccc ggccgccttt tttttcgcgc cgtgatgaag aagccggtca gccgcgtcct
     8881 gatcatcaac gacgagccgc tggtcctgaa ggagttcgtc aagggcctga acgccgcggc
     8941 acgctccctc gacaatcccg tcggcatcac gttccagggc gtgaccacgg cgcgcgaggc
     9001 gctcgccgtg atcgagcgcg acggcgacct gcaggcggtg gtggtcgacg acaagctcta
     9061 caccctcaag aatccaagca cgaagaagga gcaggcgtct gcgctcgagc tcgtccagcg
     9121 catcacgcgc ttccgtccgg agctcgacgt ctacatcctg atcgcgcagg agcaggagga
     9181 cgagatcgtc gacacgctgt tcgccgaggc cgtcgacggc tacttctacc gcgaggagcg
     9241 cgactaccgc ggcatgtacc gtatcctgaa cgcgcagatc caggagcgct cgcgcacgcc
     9301 cttctacgac cagctcaaga actacgtctg gatggcgaag gactcgtggc acacgccggg
     9361 gcactcctcg ggcgagagcc tgcgcggcag cccgtgggtc aacgacttct acgacttcat
     9421 gggcgagcac gtgttcgacg cggacctgtc ggtctcggtg aagatgctcg actcgctcat
     9481 ggagccgacc ggcgtgatcg ccgaggccca gaccatcgcg gccaaggcgt tcggcgcgcg
     9541 gcgcacgttt ttcgcgacca acggcacttc gacggcgaac aaggtcatct tccagacgct
     9601 gctcgcgccc ggcgagaagc tgctgctcga ccgcaactgc cacaagtcgg tgcaccacgg
     9661 cgtggtgctc tcgggcgccc acccgatcta cctggactcg tcggtcaacg ccaagtacag
     9721 cctctacggc ccggtgccga agaagaccat cctcgccgcg atccgcaagc acccggacgc
     9781 gcaggcgctg atcctcacct cgtgcaccta cgacggcttg cgctacgacc tgaagcccat
     9841 cgtcgaggcg gcgcacgcga agggcatcaa ggtgatcgtc gacgaggcct ggtacgggtt
     9901 cgcgcgcttt cacccgtcgt tccgtccgac cgcgctcgag gcgggcgcgg actacgcgac
     9961 gcagagcacg cacaaggtgc tttcggcgtt ctcgcaggcg tcgatgatcc acgtcaacga
    10021 cccggcgttc aacgagcacc tgttccgcga gaacttcaac atgcatacgt cgacgagccc
    10081 gcagtacagc atgatcgcga gcctcgacgt cgcccgcaag caggccgtga tggagggcta
    10141 caagctgctg tcgcgcacgc tgatgctgtc gcaggagctg cgcggtcaga tcaactcgac
    10201 cggcgtgttc cgggtgctcg agctcgagga gctgctgccc gaaaaagtcc gcagcgacgg
    10261 catccgtctc gatcccacga aggtgaccgt cgatatcacg agctgcgggt atacggtcga
    10321 agacctgcaa aggcagctct tcgaccggta caacatccag gtcgagaaat cgaccttcaa
    10381 cacggtgacc ctgctgctca ccatcggcac gacccgcagc aaggtctcgc gcctctacga
    10441 cgcgctcatg cgcatcgccc gagagggccg ggcgccgcgg cggctctacc gcttccccga
    10501 gattcccggg ttcacgcggc tgcgctacct gccgcgggat gcgttctact gcggcggcga
    10561 gctggtgccc ctggtcgacg agcgcgaccg cgtgcgccgc gagctgctga accgggtgtg
    10621 cgcggaccag atcgtcccct accccccggg aatacccgta ctcgtgccgg gccaggtcat
    10681 cacccgggac atcgtcacgt acctggtcgg gctgctgcgg tcgcagaacc gggtggagct
    10741 gcacggcatc gtctacgacg gctatctgcc ctgcctcagg gtgctcaagc ccgccgagga
    10801 gaaagggctc caggcgctcg cctgagcggc agcgcaaaaa tcacgtgctt tccccggggc
    10861 cgtgtgctac aacacgacaa aagacccgag gggagcaggg ttatgtcgct gtcttcgacg
    10921 accacaaaat acgggttccg catccgcacc cgcgacggca tggtggtcga gcacctcatg
    10981 atccacggcc gcgacgaagc cgacgccgag cgcaagctcc gccagatgta cctccactgc
    11041 gagatcctcg agtgcagcat catgcagccc gcgatgatgc agccggccaa gcgctcctca
    11101 gccgcggccg ccagcttcga agacgtgatc tcgctgatct cgaaatagcg ctgacagcgc
    11161 acgccgtcgg ctcagacgtt ccagaacccg ctcgaacgaa gctcctccag cagccgcgcg
    11221 tagtcctgcg cgccgcgacc gtcggggtcg tggctgaaga cgtccttgcc gaacgccggg
    11281 ctctcggcga taccgaccga ctccgcgatg cgcgtctcgc acagctcggc gccgaagcgc
    11341 tcgccgagct gggccgcgat gtcgtgcgcc atgcgccgcc gcgagtcgaa gcgcgtgatc
    11401 acgtagcgcc gctcgacgcg ctgcttgagg acgtgctcga gcgccttcaa ggtcttctcc
    11461 agcgccaccg cgcccttcat agcgagatag tcggccgaca ccggcaccag cacgcgctcg
    11521 gtcgcgaaca ccgcggatag cgagagcacg cccagcatgg ggctgcaatc gatcacgacg
    11581 ggctgcgagc tctcgtaatt ggcatccagg ccctgcttca ggcggttcag gacgtcgggc
    11641 cccttgccga acatcgagtc gatcttgatg agctcggtgt gcgcgggcag gagctgcccg
    11701 ccgaaggcca cgggccgcgc gatgcccgcg agcggcttcg attcgctgta cagggtgaag
    11761 aggctctcct tgctcgagga gatggggttc aggatggacg tcagatgcgc ctgcgcgtcg
    11821 agatcgacca gcaccggcaa caggccgatc cgggcgagcg ccgcgcccag gttgagcgtc
    11881 gtggtcgtct tgcccacgcc gcctttctgg ttgaacactg tgaggcgcgt tcccatcccg
    11941 gagtgattat gctaagctgt tggcgtttca gagaaatttt atcgcactga gcgacgacat
    12001 gatcggatat ttgcaaaagg gcgcgccccc gaccggcgcg tcgtcgttca ttcggcgcga
    12061 cacccgacga cgccgcccac ccgcgtagac cgaaccgcgc gcgccgcaac gcggcgtgcc
    12121 tcgagcaacc gggtgcggcc caaggccgcc cgagaacaga acgacgggtg actcatgtcc
    12181 catgtgatga atacttacag ccggcttcct gtttccttta cccgcgggga aggggtatgg
    12241 gtctgggacg acgcgggtcg gcgctatctc gacgcgctgg ccggaatcgc cgtgaacacg
    12301 ctcggccacg cccatcccgg gctggtgcgc gtgatcgccg agcaggcggc gcggctcatc
    12361 cacgtgtcta acctgtacgg catcgccgag caggagaagc tcgccgacaa gctcgccgcg
    12421 atcagcggga tggacaacgt cttcttctgc aactccggct gcgaggcgaa cgaggcggcg
    12481 atcaagatcg cgcgcctcta cggccacaag aaagggatcg agtcgcccgc gatcgtggtc
    12541 atggacaagg cgttccacgg caggacgctc gcgacgctct cggccacggg caaccgcaag
    12601 gtgcagaagg gattcgagcc gctcgtgccc ggcttcgtgc gcgtcccgtt caacgacatc
    12661 gaggccgtgc gccgagtggc ggcggcggac cggaatatcg tcgccgtcct ggtcgagccc
    12721 atccagggcg aaggcggcat caacatctcg agtctccagt acctgcgcga gctgcgcgag
    12781 ctgtgcgacc gccagggctg gctgctcatg gtcgacgagg tgcagtgcgg cctcggccgc
    12841 accggccgct ggttcgtcta ccagcacgcg ggcgtgctcc ccgacgtgat gccgctcgcc
    12901 aagggcctgg ggtcgggcct gccgatcggc gcgtgcctgg cgcgcggcgc ggcggcgcgg
    12961 gtgttcgagc cgggcaacca cgggtcgacc ttcggcggga acccgctcgc gtgcgcggcg
    13021 gcgttcgcca cgctcagcat cgtcgaggag gaaggcctgc tcgagaacgc cgcgcgcatg
    13081 ggcgccctgc tgctctccgg gctgcgcgag cagctcgccg gcgtccccgg cgtcgccgac
    13141 gtccgcggcc acgggctgat gatcggcatc gagctcgacc gcccctgcgg cgtcctcgtc
    13201 ggccgcgcgc tggagcaggc gctgctgatc aacgtcacca tggacaatgt ggtgcggctg
    13261 cttccggcgc tcgtcatctc ggaggccgag gtgcgtcagc tgctgtcggg cctggtgccg
    13321 ctgatccgcg gcttcctcgc cgagcccgcc gcggcggcga gcgccgcgcg cgcctgacat
    13381 gccggccgag cacaaaccgc ggcactacct ggaattccgc gacctgcagc gcgaggaata
    13441 cgagcacgtg tacgcgcgca cgcgctggat caaggacagg ttcaagcgct acgagcgcta
    13501 ctggccgctc gaggaccgga cgctcgcgat gatcttcgag aagccctcga cccgcactcg
    13561 catgtccttc gaggcgggca tgcaccagct cggcggcgcc gcgatctacc tgtacacgcg
    13621 cgactcgcag ctcggccgcg gagagccggt cgaggacgcg gcgcaggtga tctcgcgcat
    13681 gtgcgacctg atcatggtgc gcactttcga gcaggacatc atcgagcgct tcgccgccaa
    13741 ctcgcgcgtc ccggtgatca acggcctcac caacgagtac cacccgtgcc aggtgatggc
    13801 cgatatctac acctacatcg aggagcgggg gccgatacgc ggaaagacgg tcgcgtggat
    13861 cggcgactcg aacaacgtct gcaacacctg gctgcaggcg gcgactatcc tggacttcaa
    13921 cgtgcacgtc tcgacgccgc cgggctacga ggtcgagccc gagcgcgccg gcatctacga
    13981 cgacagccac tacgagagct tctccgaccc catggatgcc gcgcgcggcg cggacctggt
    14041 gacgaccgac gtctggacca gcatgggctt cgaggccgag aacgaggagc gcaagcgcga
    14101 tttccaggac tggcaggtcg acgccgacat gatgcgcgcg gcgaagcggg acgcgctgtt
    14161 catgcactgc ctgcccgccc accgcggcga ggaagtcgcc gcggaagtga tcgacggccc
    14221 gcagagcgtc gtgtgggatg aggccgagaa ccggctgcac acgcagaagg cgctcatgga
    14281 gttcttgctg ctcggaaaaa tcgaacgaac ctgaaaggcg agggatgaaa ggaaaagaag
    14341 acgggatcag gaaggtcgtg ctcgcgtatt ccggcgggct cgacacctcg gtgatcctga
    14401 agtggctgca ggacgtctac cgctgcgagg tggtgacctt caccgccgac atcggccagg
    14461 gcgaggagct cgagcccgcg cgcgccaagg ccaaggcgct cggcgtgaag gagatcttca
    14521 tcgacgatct gcgcgaggag ttcgcgcgcg acttcgtgtt cccgatgttc cgtgcgaaca
    14581 cgatctacga gggcgaatac ctgctcggca cgtcgatcgc gcggccgctg atcgccaagc
    14641 gccagatcga gatcgcgcgc aagaccggtg ccgacgcggt cgcccatggc gcgaccggca
    14701 agggcaacga ccaggtgcgc ttcgagctcg gctactacgc cctcgagccc ggcatccacg
    14761 tgatcgcgcc ctggcgagag tgggacctga cgtcccggga gacgctgctc gcgtacgccg
    14821 agaagcacgg catcccgatc gaaggcaaga agaagggcgg ctcgccctat tcgatggacg
    14881 cgaacctgct gcacatctcg tacgagggcc gcattctcga ggatccttcg cgcgagcccg
    14941 aggaggacat gtggcgctgg acggtgtcgc cggagaaggc gcccgatcgg ccccaggtcg
    15001 tcgagctgga gtaccgctgc ggcgacatcg tcgccgtcga cggcaagacc ctgacgcccg
    15061 gcgctgcgct cgcgcgcctg aacgagctcg gcggccagca cggcatcggg cgtctcgacc
    15121 tggtcgagaa ccgctacgtc ggcatgaagt cgcgcggctg ctacgagacg cccggcggga
    15181 ccatcatgct caaggcccac cgcgcgatcg agtcgctgtg cctggaccgc gaggtcgcgc
    15241 acctcaagga cgagctcatg ccgcgctacg cctcgctgat ctacaacggc tactggtgga
    15301 gccccgagcg ccggatgctg cagaccatga tcgacgcgtc gcaggcgagc gtgaacggca
    15361 aggtgcgcgt caagctctac aagggcaacg tcatggtcgt cggccgacag tcggacgact
    15421 cgctgttcga tcccgcgatc gcgaccttcg aggacgaccg cggggcctac aaccaggccg
    15481 acgccgccgg cttcatccgg ctgaacgcgc tcaggatgcg catcgcggcg aagctcaaga
    15541 ccaagcgtcg ctagggctcg ggacgaccgg tcccggcgca cccggatgtg acgtcgttca
    15601 ccgtgccacg cgatcggggg cgcggataat gagcggatgc gcattctcgt cctctcggac
    15661 attcatctcg aagtggcgcc cttcgagccc gccgcctcgt ccgcggacct ggtggtgctc
    15721 gccggcgata tcgcgaacgg cggcgcgggc gtggactggg ccgagcgcag cttccgcgct
    15781 ccggtgctgt acgtccccgg caaccacgag tactacgacc acgaattcga tcgggccaac
    15841 gagtcgctga ggaaggcggc cggggcgcgc gtgcgcttgc tcgactgcaa ggaagccatc
    15901 accgacgacg ttcgcttcct cggctgcacg ctatggaccg actacacgct ggtcccggac
    15961 gcggcgcgcc ccgcggtcgt cgaagaagcg cgcgcgaaga accccgacta cgcgaagatc
    16021 cggttcgacg gccgcaactt cacccccgag cacgcgatgg cgctgtgccg caagcatcgc
    16081 gcctggctcg aacgcaagct gtacgagccg ttcggggggc gcacggtggt catcacgcac
    16141 ttcgcaccgc acccgaactc catcgcgccc gcgtacatgg ggcaccccgc caacccgggc
    16201 ttcgtgctgg acctttcgcc gctcatgggt cgcgccgcgc tgtggatcca cgggcatacc
    16261 cacaccgcgt tcgattacga ggtgaagggc acgcgggtgg tgtgcaatcc gcgcggctac
    16321 cccggggagc ctacgggctt ccgcgccgac ctgatcgtgg agatttgacc cggatctcgg
    16381 gtgtcccggt gtcgtacttg ccgtggcgca ggtcgcgccg cgcgcgctcc gcgagccggc
    16441 aatactcgcg gtggatcgcg tcgagctcct cctcctcgag ctcttcgaga tcgagcagcg
    16501 cggtatgcgc tccgttgatc gagcggatga tctcgtcgag cttcacctgg atcgcttccg
    16561 agtcgcggtt ctgggtgttc tggatcagga acaccatgag gaaggtcacg atggtggtgc
    16621 cggtgttgat cacgagctgc caggtatcgc tgaagccgaa gaccggcccg gtgacgcccc
    16681 agacgacgat gacggccgcg gcgacggcga acgcggacgg ccttcccgcg gtcctcgcag
    16741 tccatttggc gaaccgggaa aaccaggatc gcgatcgcgt tggtctcatg ggatcacctc
    16801 tcgaagcctg tcgctataat tgcccgttcc agtgaggttg acatgccctc cttcgatatc
    16861 gtttccgagg tgaacaaggt cgagctgcgt aacgccgtcg accaggcgaa caaggagatc
    16921 gcgaaccgct tcgacttccg cggctcggac gcccgcatcg agcagaacga gctggcgctg
    16981 acgttctacg cggacgacga tttcaagctc aagcaggtcg ccgagctgtt catgaccaag
    17041 tgcgccaagc gcggcgtcga cgtgcgcgcc ttcgatcccc agggtatcga gaagatcggc
    17101 ggcgacaaga tgaagcagcc ggtcaacgtc cgctccggca tcgagcagga gcgcgccaag
    17161 cagatcgtca agctggtcaa ggacagcaag ctcaaggtgc aggcgagcat ccagggcgcc
    17221 gccgtgcgcg tctccggggc caagaaggac gacctgcaga ccgtcatcca gttcctgcgc
    17281 aagcaggtga ccgacatccc ggtgcagttc aacaacttca gggattgact aggcgaggcc
    17341 ccggatccag cggtcgtaca cgcgccgcgc gatcccgttc agcgcgtcga cgcggcgacc
    17401 gaggtccatc tgcatctcgg ccggcgtctg caccgaaggc gccctgccga cgatgccctc
    17461 ctgcttccag ccgcggcacc aggttcggat catctcgggc gtcatctcga cgtggcactg
    17521 catcccgaga tggttgccga gcgcgaacgc ctggttgacg caatacgggc tcgccgcgag
    17581 gcgcacggcg cccggcggga tcgagaaggt ctcgccgtgc cagtggaacg aatcgaactg
    17641 cttcaggtcg ccgaaccact cgcgcgcctg gggattggcc tccaccgtca cgcgacccca
    17701 gccgatctcc ttcaccggat tgcgctcgac cctgccgccc agcgccctgg atatcaactg
    17761 gccgccgagg caatgcccga gcacggggat gttggccctc accgccgcgt cgatcaacgc
    17821 gagcacccgc gggatccacg gcagctcgtc gttgacgctc atcggtccgc ccatgaagac
    17881 caggcccgaa taagggccgg ggtcggccgg aacgggctcg ccggcgtcga tcttcacgag
    17941 gcgccagggt atgcggtgat cgtcgaggta tgtggcaaaa taccccggcc cttcggtgcc
    18001 gaaatggcgg aaaatcgcaa cgggcttcat ggagaaagcg atgaaccgga tgcgtcatag
    18061 tattgcatgc tcggcgcttt tcctctgcgc cgccccggcg ctcgccaccg acgtgaacgt
    18121 cgtggggctg tttcccggca aagcggtcgt ctcgatcaac ggcgggcagc cgcgcaccct
    18181 gcacgagggc cagaaaaccg acgagggcgt gacgctgctc ggcaccgagc gcgaccaggc
    18241 caccctcgag atcgacggcc gcaagcgcgt gctgaagatg ggtcaggtct acgtcgccgc
    18301 gccgggcggc tcctcgcagc agaccgtgac actggccgcc gacagccgcg ggcatttctt
    18361 cgtgaacggc caggtgaacg gggggcagat gcgcttcctc gtcgacaccg gcgccacgtg
    18421 ggtcgcgctc tccaccgccg acgcggtgcg cctcgcgatc gactaccgca agggcgagcc
    18481 gaacctcttg tcgaccgcga acggcgtcgc agccgcctac aaattgaagc tggataccgt
    18541 gcgcgtgggc gacatcacca tcagcaacgt cgacgcggtg gtcatggacg gcgcgagccc
    18601 gggcgtcgcg ctgctcggca tgagcttcct gaaccgcatg caaatgcgcc gcgacggcgg
    18661 cacgatggtg ctgacgaagc ggttctagcg ggcgggcttt tcccgcttgt ccttctcgat
    18721 cctggcgtat gccgagtggt tgtggatcga ctcgaagttc tctgactcga ctacgtacca
    18781 gtcgatgcgg tcgtcgcggt tgagctccgc ggcgacgtcg cgcaccaggt cctcgacgaa
    18841 cttcgggttg tcgtaggcgc gctcggtgac gtgcttctcg tcggggcgct tgagcaggcc
    18901 gtagagctcg ctcgaggcgt tgcgctcggc gatctccacc agctcctcga tccacacgaa
    18961 gccgcgggtg cgcgcgctga tcgtcacgtg cgagcgctgg ttgtgcgcgc cgtactcgga
    19021 gatgcgcttg gagcacgggc acaggctcgt gaccgggacg accacggtca tcttgaagca
    19081 cttgctgccc ttctcgacct cgccggtgaa ggtcacgtcg taatccatca ggctctgcac
    19141 gcccgacacg ggcgccgcct tgctcacgaa atacgggaag cgcatctcga tgtgccccga
    19201 ttcggcctcg agacgctgca ccatctcgcc cagcatcagc ttgaaagtgt cgaccgagat
    19261 ctcgcgctcg tgcgcgttca ggatctcgac gaagcgggac atgtgcgtgc ccttgaactg
    19321 gtgcggcagg cccacgtaca tgttgaacat ggcgaccgta tgctgcgtgc cgccgccgcg
    19381 ctcggcgatg cgcacgggat ggcggatgcc cttgatgccg accttgtcga tcgcgagctt
    19441 gcgcttgtcc tcggcgctct ggacgtcggg aatggcgagg gggtcgcggg tattcatgga
    19501 tcgagcctct cttcgttggc tcagattgga tgattttaac atgcggcccg ttccggccct
    19561 tttcaacgct gcagcggcgc gacgcattcc cgcaccgcga tcgcgggaat cgttcaggcg
    19621 agccgggcgc gcacggcggc ggcgatgccg cctgcgtcga gccccaccgt cgcgaggagc
    19681 tgcccctgat cgccgtgatc gacgaagcgg tcgggcaggc cgagccgcag gacaggggtg
    19741 gcgatcgctg ccgcggaaag cgcctccgcg accgcgcttc cggctccgcc catgacggcg
    19801 ttctcctcca ccgtgacgat cagctcgtgc tcgcgcgcca tgcgcgccgc gaggtcggcg
    19861 tcgagcggct tcacgaaacg catgttcacc accgtggcgt cgagcgcgtc gcccgcctcg
    19921 agcgccggct tgagcagcgt gccgaacgcg aggatcgcca cgcggcgtcc cttgcggcgt
    19981 acctcgcctt ccccgaacgg gagcggctcg agctcgggac gcaccgcgac acccggcccg
    20041 ctgccgcgcg gatagcgcac cgccgagggc tgatcgagtc ggaatgcggt ggtgagcatc
    20101 cggcggcact cgttctcgtc ggccggcgtc atcaccacca tgttcggcag gcacctcagg
    20161 taggagagat cgaacgcgcc gttgtgcgtc gcgccgtcgc cgccgacgag ccccgcgcga
    20221 tcgagcgcga acacgatcgg cagattctgg atgcaaacgt cgtgaatgag ctggtcgtag
    20281 ccgcgctgca ggaacgtcga gtagatcgcg accacgggct tcaatccctc gcacgcgagg
    20341 cccgccgcga acgtgactgc gtgctgctcg gcaatgccca catcgaagta gcgatcggga
    20401 tgctccttcg cgaagcgcac gagccccgag ccctcgcgca tcgccggcgt gattcccacc
    20461 aggcgtctgt cggccacggc catgtcgcac agccagtcgc cgaacacctg ggtgaacgtg
    20521 accttgccgc cgctcgcctt cttgatgccc tcggcgggat cgaacttgcc cgggccgtgg
    20581 tagagcaccg ggtcggcctc ggccatcttg tatccctggc ccttcttcgt gaccacgtgc
    20641 aggaactgcg gacccttcag ctccttgatg ttcttcagcg tcggcaccag cgagtcgagg
    20701 tcgtgcccat cgatcgggcc gacgtagttg aagccgaact cctcgaacaa cgtcgagggc
    20761 acgaccaggc ccttcgcgtg ctcttcgacc ttgcgcgcga gctcgcgcag gctgggcacg
    20821 ccggcgagca ccttctccgc cccgcgcttg gccgcggcgt agaagcggcc cgacatcagc
    20881 cgcgcgagat agcggttcag cgcgccgacc gcaggcgaga tcgacatgtc gttgtcgttg
    20941 aggatcacga gcaggtcggt ctccatcacg cccgcgttgt tcagcgcctc gaacgccatg
    21001 ccggcggaca tggcgccgtc gccgatcacg gcgatgcagt ggcgcggctc acccgcgagt
    21061 ttggcggcct gcgccatgcc gagcgcggcg ctgatcgagg tgctcgaatg cgcggtgccg
    21121 aaggtgtcgt aggggctctc gccgcgccgg ggaaatcccg agatgccgcc cagcatccgc
    21181 aggcgcgaca tcgcctcgcg ccggccggtg aggatcttgt gcggataggt ctggtgcccg
    21241 acgtcccaga cgatgcggtc gtagggcgtg tcgaacacgt aatgcagcgc gatggtcagc
    21301 tcgaccgtgc cgagattgga cgacaggtga ccgcccgtct ggctcaccga ctgcagcacg
    21361 aactcgcgca gctcgtcggc gagctgcgcg agctgccggc gctcgagctt gcgcaggtcc
    21421 gccggatcgt tgatcgtctt cagcagctcg taccgcttgg ggtcgtagcc gcacgagccc
    21481 gcgacgccgc cgtcgccggg caaatccgct tctccgatgg cgctcataag cctgaattat
    21541 ccctcaagtg cactagcctc gctgcctcgt ccgttggacc gctccgcccg gcttcgtgct
    21601 ggatctcgtc ctagaactcg cgcagcacca cgaagtcggc gagatccgcc aggcgccgcg
    21661 ccgccgcgcc gaacgggcgc agcgccgcgg ccgcttccgc gcgcagctcc tcggcgagcg
    21721 ccctggcgcg cgcggttccg agcaccgaga cgtaggtcgg cttgttgtgc gcggcgtcct
    21781 tgcccgcggt cttgccgagc gtggccgtgg atccttcccg gtcgagcacg tcgtcgacga
    21841 cctggaacgc gaggccgatg cacttcgcat agcgctcgag ctcgcgcagc tgctcctcgg
    21901 gcagcggccg cccgcagcgc gcgccgagca cgacggaagc gcggatcagc gcgcccgtct
    21961 tgtgcacgtg catgtgctcg agctcgggca gcgtcaggct ccggccgatg ctcgcgaggt
    22021 cgatggcctg gccgcccgcc atgccgcggc tgccgctcgc cgcggcgaga actcgcagca
    22081 tctcgagctg cacgcccggg tcgtcggcga gacggtgctc ggcgagcagc tggaacgcga
    22141 gggactggag gctgtcgccg acgaggagcg cggtcgcctc gtcgtactcg acgtggcagg
    22201 tcggtttgcc tcgccgcaga acatcgtcgt ccatgcacgg gagatcgtcg tgcacgagcg
    22261 aatagctgtg gatgcactcg accgcgcagg cgactacctc gaggcgcgaa ggatccgcgc
    22321 ccgccagctc gcccgccgcg aacgcgagca gcgggcgcac gcgcttgccg ccgccgagca
    22381 ccgcgtagcg catcgcctgg tgcaggcgct gcggcacgat ctcgcccgaa ggcaggaagc
    22441 gcccgagcgc ggcttcgacc cggccctgca cctggcccat ccaggccatg aagtgcgcag
    22501 ccgcgtcagt gctcgggcgg ctcaccgggc agcgtcttga gcgtgttctc ttccaggacc
    22561 ttgacctgct gctgggcccg ggagagcacc gactgacaat actgcgcgag ctccagcccg
    22621 cgcttgtggg ccgagagcga ctgctcgagc gtgagcttgc ccgactccat gctggcgacg
    22681 agcttctcca gctcggagag cgcggcttcg aagctggcgg gagtttcggc gggcttgctc
    22741 atcggtcctg cgggctcggg aatcattgcg aagcgggcgg gtccgtcaaa atagcgtatg
    22801 gggatacgcg ggtcaaatcg atcgcacaaa accctggatt acacagggga aaagggctag
    22861 aatcgcgccc attttatttc cacgggacgt tgatccgggc gccaggcgcc cccaaaccgc
    22921 agatatgtcc gcgcatttat gtccgagata gccacgacgt cgaaactccg gcccagcacc
    22981 gcgcaacttc cggtgtcctg gtacttcgat tcccgcatct tcgatctgga gcaaaggctg
    23041 ctcttcgaga caggaccgaa gtacgtcggc cacgagttga tggttcccaa tccgggggac
    23101 tatcacacgc tgtcgtggat ggaccatggc aaggcgctgg tgcgcaacac gaacggcatc
    23161 gagctgctgt cgaacgtgtg ccgccatcgc cagtcgatcc tgctcgaggg ccggggcaat
    23221 gcgcagaaca tcgtctgccc gctgcaccgc tggacctacg acctgaaagg cgagctgctc
    23281 ggggcgccgc acttcgccga caatccctgc ctcaaccttc cgcgcacgcg gctgcagtcc
    23341 tggcagggca tgctgttcga gggcggccgc gacgtcgccc gcgacctcgc gaatttctcg
    23401 ctcgcgcccg acttcgattt ctcgggctac ctgttcgacc acgtcgaggt gatggagctc
    23461 gagcagaact ggaagacctt catcgaggtg tacctcgagg attaccacgt cgtgccttat
    23521 cacccggggc tcggcggctt cgtcacctgc gacgacttgc gctgggagat cgcggacacg
    23581 tattcggcgc aggtggtcgg cgtcaacaac ggcctgaagc agccgggcac tcccgcgtac
    23641 gcgaaatggc acgaggcgtg cacgaagtac ctggacggcc gcgcgcccaa gcagggcgcg
    23701 atctggatgc tgtactaccc gaacgtcatg ctcgagtggt acccgcacgt gctggtcgtg
    23761 agcacgctgc tcccgcgctc gcccacgctg accacgaacg tcgtcgagtt ctactatccc
    23821 gaggacatcg tgctgttcga gcgcgccttc atcgaggccg agcgcgctgc gtacatggag
    23881 accgcgatcg aggacgacga aatcgccaag cgcatggacc ggggccgccg cgcgctcatg
    23941 ctgcgcggcg aggacgacgc gggcccgtac cagtcgccga tggaagacgg catgatgcac
    24001 ttccacgagt acatccgcag gggcctcgcg cccggactct gacgcccggg cgggcccgcg
    24061 gcggtaacat ggccgcatga gctacaccac gctggtctcc accggggtcc tcgcgcggca
    24121 cctcgacgac ccggcctggg tcgtgttcga ctgccgccac gatctcgccg atcccgcacg
    24181 cggccgccgc gagtacgcgc ggtcgcacat tccgggcgcg cgcttcctgc acctggacga
    24241 cgatctttcg tccccgtgca cgggaacgaa cggccggcac cccctccccg atttcgccgc
    24301 gctcggccgc aagctcgcgg gcgcgggcgt cgcgcccgga gtccaggtgg tcggctatga
    24361 cgcgcagggc ggcatggtgg ccgctcgcct gtggtggctg ctgcgcggcc tcggtcacga
    24421 ccaggt
//
LOCUS       DATDMW010000043         3542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_384506, whole genome shotgun sequence.
ACCESSION   DATDMW010000043 DATDMW010000000
VERSION     DATDMW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384506"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VI319_01805"
     CDS             <1..257
                     /locus_tag="VI319_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862608.1"
                     /translation="RTDELQFSFDIVAANGHTCAMSGTAARKGAVYVYSKRVGEGQCL
                     LEISVSADAVTLHDAGMICRTEYCGFRGRIDGTSFRRSAP"
     gene            254..733
                     /gene="ispF"
                     /locus_tag="VI319_01810"
     CDS             254..733
                     /gene="ispF"
                     /locus_tag="VI319_01810"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY6862609.1"
                     /translation="MSHRIGQGFDSHALAAGRRLVIGGVEIPHAKGLAGHSDADVLIH
                     AICDALLGAAALGDIGRHFPDTDPKYKDADSRTLLRAVAQKIREAGYDIANVDATVIA
                     EAPKLAPHIPAMVKSLAADLGVGERSINVKAKTAEKMGALGRGEGIAAQAIALLAPK"
     gene            730..1221
                     /gene="thpR"
                     /locus_tag="VI319_01815"
     CDS             730..1221
                     /gene="thpR"
                     /locus_tag="VI319_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY6862610.1"
                     /translation="MRLFFALWPNAVTRTRLDRWGQALHDVCGGRRTSADSLHLTLAF
                     LGNVADARVAEVESAMDRVRPRRFLLNLDTPGHWKKNSIAWAGTSAVPAELEALAADL
                     RAALAAATIPFDPQPFAVHVTLLRDAGAPAAMPALEPIAWPVDGFALVSSAGGRYEIR
                     RSR"
     gene            1313..1885
                     /locus_tag="VI319_01820"
     CDS             1313..1885
                     /locus_tag="VI319_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862611.1"
                     /translation="MKRIIPVVLVALLLGCTASLGPVAKLDDKDVSVRDQRQDAEKKS
                     FRSGMTSPVILLGDENFDVNPMLYLQEALQRHKPPAAGSLQMVVRKFRLIDYFPKRQA
                     SAQQAALVGVMTSMGYSVTSVSSARKDEDSIICVLEGSLNGRAVQASVSTPYKLNSAA
                     AAVRSDANWIAAARKSIDQCVAAAVSQAVP"
     gene            2068..2757
                     /gene="modA"
                     /locus_tag="VI319_01825"
     CDS             2068..2757
                     /gene="modA"
                     /locus_tag="VI319_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6862612.1"
                     /translation="MAADLEVIAGGGIAGPLKEIVAKFEQATGHKVAVTFGTTPELIK
                     MAAGGQRFDVGVVPVEFTKDDAARATFLAPTTRELARVGMGVAVKAGAPKPDIGTVEA
                     LKRTLLAAKSIASIPASAAGGLLERSYQRMGIADAVKAKIRPQPTPADIVKSVASGEA
                     ELGVFLTNVLTAPGVDLVGPFPAEVQQDLVYTAALSARAREARAAEQFLAYLRGPEGA
                     AIIKAKGMTPS"
     gene            2754..3182
                     /locus_tag="VI319_01830"
     CDS             2754..3182
                     /locus_tag="VI319_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY6862613.1"
                     /translation="MNRGKARSASTRSLPASRLIDGRIAELGDWRGETLARMRRLIRD
                     ADPGIVEEWKWDGPVWSRDGVICTGETYKSVVKLTFLKGASLKDPTKIFNSSLEGNAR
                     RAIDIREGETVDAEAFKSLIRAAVAANASGAPTKRAKKKS"
     gene            3193..>3542
                     /locus_tag="VI319_01835"
     CDS             3193..>3542
                     /locus_tag="VI319_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862614.1"
                     /translation="MTFHSTSVIACASLALVCACAGPTRPAAPPDERTSFVMEQNWAK
                     LDRPWGSTSAVDVDGAGHVWVFERCGANSCAGSSLDPVLKFDGGGRLVAHFGAGLMIA
                     PHGIFVDHDGNVWVT"
BASE COUNT          589 a         1190 c         1250 g          513 t
ORIGIN      
        1 agaggacaga cgagctgcag ttttccttcg acatcgtcgc ggctaacggc catacctgcg
       61 ccatgagcgg cacggcggcg aggaagggcg cagtgtacgt gtacagcaag agagtcggcg
      121 agggacagtg ccttctggag atttccgttt ccgccgacgc ggtgacgcta cacgacgcgg
      181 gcatgatatg ccggacggaa tattgcggct tccgcggccg aatcgacgga acgagcttca
      241 ggagatctgc gccatgagcc atcgcatcgg ccagggcttc gacagccacg cgctcgccgc
      301 ggggcgcagg ctcgtcatcg gcggcgtcga gatcccgcac gcgaaggggc tcgccggcca
      361 ctccgacgcc gacgttctca tccacgcgat ctgcgacgcg ctccttggcg cggccgcgct
      421 cggggacatc ggccgccact tcccggacac cgacccgaaa tacaaggatg cggacagccg
      481 gacgctgctg cgcgcggtcg cgcagaaaat cagggaggcg ggctacgaca tcgccaacgt
      541 cgacgccacc gtgatcgccg aggcgccgaa gctggcgccg cacattcccg cgatggtgaa
      601 gagcctcgcc gccgatctcg gcgtcggcga gcgcagcatc aacgtcaagg cgaagaccgc
      661 cgagaagatg ggcgcgctcg gacgaggcga gggcatcgcc gcgcaggcga tcgcgctgct
      721 cgcgccgaag tgaggctctt cttcgcgctg tggccgaacg cggtcactcg aacgcggctc
      781 gaccgctggg gccaggcgct gcacgacgtc tgcggcggcc gtcgcacgag cgccgacagc
      841 ctgcacctca cgctcgcgtt cctcgggaac gtcgccgacg cgcgcgtcgc ggaagtcgag
      901 tcggcgatgg accgggtccg gccgcggcgc ttcctgctca atctcgacac gcccggccac
      961 tggaagaaga acagcatcgc gtgggcgggc acgtccgccg tgccggccga gctcgaggcg
     1021 ctcgccgcgg acctgcgcgc cgcgctcgcg gccgcgacca tcccgttcga cccgcagccc
     1081 ttcgccgtgc acgtcacgct gctgcgcgac gcgggcgcgc ccgcggcgat gcccgcgctc
     1141 gagccgatcg cgtggccggt ggacgggttc gcgctcgtat cgtcggccgg agggcggtac
     1201 gagatccgga ggtcccgctg acaatttcca cagttgactc ggtatcaccg ccgagctacc
     1261 gtgggcggca gaaccgtatg gcgcggcgca cgaacgccgc cgagggaggc tcgtgaagcg
     1321 aatcattccg gtcgttctgg tcgctctgct gctcggatgc accgcctcgc tgggccccgt
     1381 cgccaagctg gacgacaagg acgtttcggt gagagatcaa cgccaggatg cggagaagaa
     1441 gtcgttccgg tccggcatga cgtcgccggt catcctgctc ggcgacgaga acttcgacgt
     1501 caacccgatg ctttacctgc aggaggccct ccagaggcac aagcccccag cggccggcag
     1561 cctccagatg gtggtcagga agttcaggct gatcgattac tttcccaagc gacaggcgtc
     1621 tgcgcagcag gctgcgctgg tcggcgtgat gacgtccatg ggctactccg tcacgtcggt
     1681 ttcgtctgcg cgcaaggacg aggattcgat catctgcgtc ctcgagggat cgctgaacgg
     1741 ccgcgccgtc caggcgtcgg tctccacgcc gtacaagctc aactcggccg ccgcagcggt
     1801 ccgaagcgac gcgaactgga tcgcggccgc gaggaagagc atcgaccagt gtgtcgcggc
     1861 ggcggtttca caggcggttc cgtagacccc ggaaattcgc caccctcgaa tcccaaggcc
     1921 ggttcccggc gctggcgatc gacggcttcg gcatggagcc ggaaggcagg gcgatcgagt
     1981 cgacccgtct agccgaggag cgataacatg aacggatgga aagcagtgct tctgggagct
     2041 tcggcctttt gcgcggcgcc gtcgtccatg gccgctgacc tcgaggtcat cgccggcggc
     2101 ggcatcgccg ggccgctgaa ggagatcgtc gcgaagttcg agcaggcgac cggtcacaag
     2161 gtcgcggtca cgttcggcac gacgcccgag ctgatcaaga tggcggcggg cgggcagcgc
     2221 ttcgacgtcg gcgtcgttcc ggtcgagttc acgaaggacg acgcggcgcg cgcgacgttc
     2281 ctcgccccga cgacgcgcga gctggcgcgc gtcgggatgg gcgtcgcggt gaaggctggg
     2341 gcgcccaagc ccgacatcgg cacggtcgag gcgctgaagc ggacgctgct cgcggcgaag
     2401 tcgatcgcgt cgatcccggc cagcgccgcg ggcggcctgc tcgagcgcag ctaccagcgc
     2461 atgggcatcg ccgacgcggt caaggcgaag atcaggccgc agccgacgcc cgcggacatc
     2521 gtcaaatccg tcgcgagcgg cgaggccgag ctcggcgtgt tcctcacgaa cgtgctgacc
     2581 gcgccgggcg tcgacctcgt cggcccgttc ccggcggagg tgcagcagga tctcgtgtat
     2641 accgccgcgc tgtcggcccg agcgcgcgaa gcgcgggccg cggagcagtt cctcgcgtat
     2701 ctccgggggc ccgagggcgc cgcgatcatc aaggcgaagg ggatgacgcc gtcgtgaatc
     2761 gagggaaggc gaggtcggcc tcgacccgga gcctcccggc gtccagactc atcgacgggc
     2821 gcatcgccga gctcggcgac tggcgcggcg agaccctcgc gcgcatgcgc cggctcatcc
     2881 gggacgccga tcccggcatc gtcgaggagt ggaaatggga cggcccggtc tggtcgcgcg
     2941 acggcgtgat ctgcaccggc gagacctaca agtcggtcgt gaagctcacc ttcctcaaag
     3001 gcgcttcgct gaaggacccg accaaaatct tcaactcgag cctcgagggc aacgcgcggc
     3061 gcgcgatcga catccgcgag ggcgagacgg tcgacgcgga agcgttcaag tcgctgatcc
     3121 gcgccgcggt ggcggccaac gcatcgggcg ctcccaccaa gcgcgcgaag aagaaaagtt
     3181 gacggagccg acatgacttt tcacagcaca tcggtaatcg cctgcgcctc gctcgcgctc
     3241 gtctgcgcct gcgcgggccc gacgcgcccc gcggccccgc ccgacgagcg cacgtcgttc
     3301 gtcatggagc agaactgggc gaagctcgac cgcccctggg gatcgaccag cgcggtcgac
     3361 gtcgacggcg ccggccacgt gtgggtgttc gagcgctgcg gcgcgaacag ctgcgcgggc
     3421 tcgagtctgg atccggtact gaagttcgac ggcggtgggc ggctcgtcgc acattttggt
     3481 gcgggattga tgatcgcccc gcacgggatc ttcgtcgatc acgacgggaa cgtctgggta
     3541 ac
//
LOCUS       DATDMW010000044         8541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_633622, whole genome shotgun sequence.
ACCESSION   DATDMW010000044 DATDMW010000000
VERSION     DATDMW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633622"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VI319_01840"
     CDS             <1..661
                     /locus_tag="VI319_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY6862615.1"
                     /translation="RFAQPRKPNQMELDPKIFRHYGDQLELEHRHRLAGREGAVRLLA
                     FRNRARMASYADALDAASGGTPDLNAVRDGEKTKTGYGVNLEQALSADVGAFLRASRA
                     DGRTETYAFTEIDRSMSGGVLIKGAAWQRARDAVGIAAVRNGLSDSHRAYLAAGGLGF
                     FLGDGQLSYRPEQIVEAFYSFAATQALTLSADFQSIRNPGYNADRGPVRVASLRVHAE
                     F"
     gene            complement(666..1286)
                     /locus_tag="VI319_01845"
     CDS             complement(666..1286)
                     /locus_tag="VI319_01845"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6862616.1"
                     /translation="MSRADRNHWDTVYRTKAAETVSWYRAHLERSLALIERVAPVRSA
                     AIIDVGGGESTLVDDLLGKGYRDVTVLDISAAAVDFSRKRLGARAEAVAWITADVTRA
                     ALHPRRYDVWHDRAVFHFLTDPAQRAAYVRQVASSVKPGGHVIVATFGPEGPEKCSGL
                     DVVRYDADALHGQFGAQFKLVESDTEVHRTPAGATQQFLYCLCRVE"
     gene            complement(1357..2271)
                     /locus_tag="VI319_01850"
     CDS             complement(1357..2271)
                     /locus_tag="VI319_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011205586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY6862617.1"
                     /translation="MGREFSGARGCVLAIHGGAGVIKRERLSGALESRYRAALERALR
                     AGHAVLAAGGPALDAVVAAVKVMEDSPLFNAGRGACANADGRHELDASIMDGATLRAG
                     GVTCVSRVRNPVEAARAVMERSPHVLLAGAGAERFARAHGAAMAPAGYFVTRSRVASR
                     ERVRRGDDAHGTVGAVARDEAGHLAAATSTGGIAGKLPGRVGDSPIVGAGTYADDAAC
                     AVSGTGMGEFYLRACLAYDVAARMRYKRESLDRASREALARMASLGGVGGLVAIDRAG
                     RVAAPFNSEGMYRGWITRAGRATVAIYR"
     gene            complement(2284..3225)
                     /locus_tag="VI319_01855"
     CDS             complement(2284..3225)
                     /locus_tag="VI319_01855"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY6862618.1"
                     /translation="MPTRLSGVLAPVVTPFKRDLSPDAARYVGHCKWLLANGCRGLAV
                     FGTNSEANSLSVDERMTLLETLVQEGVPAEKLMPGTGMCALTDSVRLTAHAVSLGCAG
                     VLMLPPFYYKGVSDDGLFRNFAEVIERVADSRLQLYLYHIPPVSQVPITLALIERLLA
                     RYPGTVAGVKDSSGDWSNTKTMLDAFAKQGFDVFAGSEVFLLENMRNGGKGCITATGN
                     INPGAINQVYERWQSSEADALQKGITATRNVMQKYPMMAALKAVVAHWARDPEWATVR
                     PPLVELAPEQKQSLVAELAARNFDMPGLGPTLAAALA"
     gene            complement(3261..4199)
                     /locus_tag="VI319_01860"
     CDS             complement(3261..4199)
                     /locus_tag="VI319_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6862619.1"
                     /translation="MNSAAEKPPAPGEPMGSLPGTMRREMARIGPIWGSDIPKHRDIV
                     LSAYAPLLAQAPKAGVVIEADRAYGAHPRQVLDVFRPQARSPAPMVIFAHGGAFVRGS
                     KTANEEVYANVLYYFARNGCVGINMEYRLAPEATFPAGAEDVALAVRWAREHAQELGA
                     DPARVFVIGHSAGASHVASYCCDPNLGRTPAEGVAGAVLLSGRLRADARPENPNAEGV
                     RAYYGADGSVYEARSPVTWAQYCPVPVMLAIAEFDNPLLDVYGAEFLWRVAAARGRAP
                     RFMRLRNHNHISMVAHFNTGEDILGREILDFIATQR"
     gene            4364..5389
                     /locus_tag="VI319_01865"
     CDS             4364..5389
                     /locus_tag="VI319_01865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY6862620.1"
                     /translation="MIVASAGYVPYSAAAIFATTVAVLIVVFYAWIRSGLNLRFSDPS
                     LTIPQMVAAGLATSYLVYESSIIARPAFVALYFIAFMFGILALDTRRLLGVAVFYVAC
                     YATAVALALPSHEADLPREAMRVGILSLILGGFSLIGGYVSRLRRKLKIANTSLTEAL
                     SASESRARIDALTGCYNRRHGREILEIESKRAARGEPLTICLADLDGFKAINDKFGHA
                     AGDEVLKGFTVTVQGGLRATDALVRYGGEEFLIVLSQTPIVEAAMVAERVRQLVEDMS
                     TVAGARGITVSIGVTEHRPPESVDETIQRADSALYRAKEQGRNRVVCASGDTSPALGV
                     ASGGRHA"
     gene            5514..6233
                     /locus_tag="VI319_01870"
     CDS             5514..6233
                     /locus_tag="VI319_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862621.1"
                     /translation="MERKRTATRRSGRALDPALLAPKAHGRAEPVVGLLPQPAHWLTG
                     GGEMGDLIRAKDWSATPLGPLHAWPESLRTTVRIVLAQRFPMLLWWGPQYIQIYNDAY
                     IPIPGAKHPQRALGVPVSECWSEIWHILRPLIDTPFKGGPSTWIEDIRLDIDRHGFTE
                     ETHFTIAYSPVPDDSAPGGIGGVLATVHEITEKVIGERRIALLRDLGAGSADARTAED
                     ACDDRVRRARSRRTRSTCPSR"
     gene            6170..>8541
                     /locus_tag="VI319_01875"
     CDS             6170..>8541
                     /locus_tag="VI319_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6862622.1"
                     /translation="MRRSRAARTLSSHAIDVPFALIYLTDEHGRRARLAATAGVAIGE
                     PVSPGAIELGAADQGEWPLARARTDSHIVEVRDLETRFARLPKDSSGAPPHTAAVIPI
                     PSALAHRPAGFLVAAVSPRLRWDEQYRSFLQLAASQIATAIANARAYDEERRRAEALA
                     EIDRAKTAFFSNVSHEFRTPLTLMLGPLADALASPGGALGGAALHAAHRNAQRMLKLV
                     NALLDFSRIEAGRVQAAFEPIDLAATTAELAGVFRSAVERAGLRLVVRCEPLGEPVYV
                     DTVMWEKIVLNLLSNAFKFTFEGEIEVALARTLDGIELKVRDTGIGIAAQDIPKIFER
                     FRRIEGAKARTHEGSGIGLSLVQELVRIHGGRIDAQSEPGKGTTFTVALKRGRGHLPE
                     ERVGAARELASTATRAQAYADEALGWLGEPADDDPAISPASAHDPDAGMRVLVADDNA
                     DMREYVARLLRARWTVATVANGEAALAALRRERFDLVLTDVMMPRLDGFGLLRAIRSD
                     PALAATPVVMLSARAGEEARIEGLDAGADDYLIKPFGGRELVARVRSQLALLAARRAA
                     ERERADLLAREQAAKREAQLQREHLVSLFMQAPNPIVMLRGREHVIQLANPPACRLWG
                     RQPAQVVGRPLLEALPDLRGQVFKELLDRVLHTGVPFEGKEVPANLDPAGDGSVETVY
                     LNFVYAPMRGVTGEIEGVLVVAFDVTDQVRAREQMTRLHAEAEQASRSKDQFLAMLGH
                     ELRGPLSPIVNALQILRLRGVQSREQDIVERQVGQITRLVDDLLDVSRIT"
BASE COUNT         1220 a         3080 c         3065 g         1176 t
ORIGIN      
        1 gcgcttcgcg cagccgcgca agccgaacca gatggagctc gatccgaaga tcttccgcca
       61 ctacggcgac cagctcgagc tcgagcaccg ccaccgcctc gccgggcgcg aaggcgccgt
      121 gcgcctgctc gccttccgca accgcgcgcg catggcgagc tatgcggacg cgctcgatgc
      181 cgcgagcggc ggcacgccgg acctgaacgc cgtgcgcgac ggcgagaaga cgaagaccgg
      241 ctacggcgtc aacctcgagc aggcgctctc cgccgacgtc ggcgcgttcc tgcgcgcgag
      301 ccgcgccgac ggccgcaccg agacctacgc cttcaccgag atcgaccgct ccatgtcggg
      361 aggcgtcctg atcaagggcg cggcgtggca gcgcgcccgt gacgcggtcg ggatcgccgc
      421 cgtacgcaac ggtctttccg actcgcaccg cgcctatctc gccgcgggcg gcctcgggtt
      481 cttcctcggc gacggacaac tgagctaccg ccccgagcag atcgtcgagg cgttctacag
      541 cttcgcggcg acccaagcgc tcacgctgag cgccgacttc cagagcatcc ggaatccggg
      601 ctacaacgcc gaccgtggac ccgtgcgcgt cgcctcgctg cgcgtgcacg ccgagttctg
      661 acggcttatt cgacccggca caggcagtac aggaactgct gcgtcgcgcc ggcgggcgtg
      721 cggtgaacct cggtatcgct ttcgaccagc ttgaactggg cgccgaactg cccgtgcagc
      781 gcgtcggcgt cgtagcgcac gacgtcgaga ccgctgcatt tctcggggcc ctcgggtccg
      841 aacgtcgcca cgatcacgtg gcctccgggc ttcaccgacg aggcgacctg gcgcacgtac
      901 gcggcgcgct gggccgggtc ggtaaggaaa tggaacacgg cgcggtcgtg ccagacgtcg
      961 tagcggcgcg gatgcaacgc tgcgcgcgtg acgtcggcgg tgatccaggc gacggcctcg
     1021 gcccgggcgc cgaggcgctt gcgcgagaag tcgaccgcgg ccgcggagat gtccagcacc
     1081 gtgacatcgc ggtagccctt ccctagcagg tcgtcgacca gcgtcgattc gccgccaccg
     1141 acgtcgatga tcgcggccga gcgaacgggc gcgacgcgct cgatgagcgc cagggaccgc
     1201 tcgaggtggg cacggtacca gctcaccgtt tccgccgcct tcgtgcggta gaccgtgtcc
     1261 cagtggttgc gatccgcccg gctcatcgat ccacggtacc ggcggtcgcg caggccgtgt
     1321 tgagataagt caagccgcga ccggcgtggc ctccattcag cggtagatcg ccaccgtcgc
     1381 gcgccccgcg cgcgtgatcc agccgcggta catgccttcg ctgttgaagg gcgcggcgac
     1441 acgcccggcg cggtcgatcg cgaccagccc gccgactccg ccgagagacg ccatgcgcgc
     1501 cagggcttcg cgcgacgcgc ggtcgaggga ctcgcgcttg tagcgcatgc gcgcggcgac
     1561 gtcgtaggcg aggcaggcgc gcaggtagaa ctcgcccatg ccggtgcccg agaccgcgca
     1621 cgcggcgtcg tcggcgtagg tgcccgcgcc gacgatgggc gagtcgccga cgcgacccgg
     1681 cagcttgccc gcgatcccgc cggtcgaggt cgccgcggcg agatggccgg cctcgtcgcg
     1741 ggcgacggcg ccgacggtgc cgtgggcgtc gtcgccgcgg cgcacgcgct cgcgcgacgc
     1801 gacgcggctg cgcgtcacga aatagcccgc cggcgccatc gccgcgccgt gtgcgcgcgc
     1861 gaagcgctcg gcgcccgcgc ccgcgagcag gacgtgcgga ctgcgctcca tcaccgcgcg
     1921 cgccgcctcg accggattgc ggacgcggga gacgcaggtc acgccgcccg cgcgcagcgt
     1981 ggcgccgtcc atgatcgagg cgtcgagctc gtgccggccg tcggcgttgg cgcaggcgcc
     2041 gcggcccgcg ttgaagagcg gcgagtcctc catcaccttc accgcggcga ccactgcgtc
     2101 cagggccggg ccgccggcgg cgagcaccgc gtgcccggcg cgcagcgcgc gctcgagcgc
     2161 ggcccggtag cgcgactcga gcgcgcccga gaggcgctcg cgcttgatga ccccggcacc
     2221 gccgtggatc gcgagcacgc agccgcgcgc gccgctgaac tcccgcccca tgggtgcggg
     2281 ggctcaggcc agcgcggcgg cgagcgtggg cccgaggccc ggcatgtcga agttgcgcgc
     2341 ggccagctcg gcgaccaggc tctgcttctg ctcgggcgcg agctcgacca gcggcggacg
     2401 caccgtcgcc cattccgggt cgcgcgccca gtgcgcgacg accgccttga gcgcggccat
     2461 catcgggtat ttctgcatta cgttgcgcgt cgccgtgatg cctttctgca gcgcgtccgc
     2521 ctcgctcgac tgccagcgct cgtagacctg gttgatcgcg ccggggttga tgttgccggt
     2581 cgcggtgatg cagcccttgc cgccgttgcg catgttctcg agcaggaaca cctcgctgcc
     2641 ggcgaacacg tcgaatccct gcttcgcgaa cgcgtcgagc atcgtcttcg tgttcgacca
     2701 gtcgcccgag gagtccttca cgccggcgac cgttccggga tagcgcgcga gcagccgctc
     2761 gatcagcgcc agcgtgatcg gcacctgcga cacgggcggg atatggtaga gatagagctg
     2821 caaccgcgaa tcggcgacgc gctcgatcac ctcggcgaag ttgcggaaca ggccgtcgtc
     2881 ggagacgccc ttgtagtaga acggcggcag catgagcacg cccgcgcagc cgagcgacac
     2941 cgcgtgcgcg gtgagccgca ccgagtcggt cagcgcgcac atcccggtgc cgggcatgag
     3001 cttctccgcc ggcacgcctt cctgcaccag cgtctcgagc agcgtcatgc gctcgtcgac
     3061 cgagagcgag ttcgcctcgc tgttcgtgcc gaacaccgcg aggccgcggc agccgttcgc
     3121 gagcagccat ttgcagtggc cgacgtagcg cgcggcgtcg ggcgagaggt cgcgcttgaa
     3181 gggggtgacg acgggggcga ggacgccgga gagtcgggtg ggcatggtcg tgacgatgga
     3241 ctcgggtgaa aggcgaatta tcagcgctgg gtcgcgatga agtccaggat ttcccgcccc
     3301 aggatgtcct cgccggtgtt gaagtgcgcg accatcgaga tgtggttgtg gttgcgcagg
     3361 cgcatgaacc gcggcgcccg gccgcgcgcc gccgcgacgc gccagaggaa ctcggcgccg
     3421 tacacgtcga gcagcgggtt gtcgaactcg gcgatcgcga gcatgaccgg caccgggcag
     3481 tactgcgccc aggtgaccgg cgagcgcgcc tcgtagaccg agccgtcggc gccgtagtag
     3541 gcgcgcacgc cctcggcgtt agggttctcg gggcgcgcgt cggcgcgcag ccgtcccgag
     3601 agcagcaccg cgccggcgac gccctccgcg ggcgtgcggc cgaggttcgg gtcgcagcaa
     3661 tagctcgcga cgtggctcgc tccggcggag tggccgatca cgaagacgcg cgcgggatcc
     3721 gcgccgagct cctgcgcgtg ctcgcgcgcc cagcgcaccg cgagcgcgac gtcctcggcg
     3781 cccgccggaa acgtcgcctc gggcgcgagc cggtactcca tgttgatccc gacgcagccg
     3841 ttccgcgcaa agtagtagag cacgttcgcg tagacctctt cgttcgccgt cttgctgccg
     3901 cgcacgaagg cgccgccgtg cgcgaagatc accatgggcg ccggcgatct cgcctgcggc
     3961 cggaagacgt cgaggacctg ccgcggatgc gccccgtagg cgcggtcggc ctcgatgacg
     4021 acgcccgcct tgggcgcctg ggcgagcagc ggcgcgtacg ccgagagaac gatatcgcgg
     4081 tgcttcggga tgtcggagcc ccagatcggc ccgatgcgcg ccatctcgcg gcgcatcgtg
     4141 ccggggagcg agcccatcgg ctcgccgggc gcgggcggct tttcggcggc ggaattcatg
     4201 cggcgagtct atcaggcggc tccccggtgt agcatagccg tgtcgagcga ccgaggaacg
     4261 agcgatccgg aacttcctgc cacaagacga caagagacag cgagtccgca tccagcgact
     4321 ggtcctctcg gcgtccgctt cggtcggcgc cgccgctctc gcgttgatcg tcgcgtcggc
     4381 gggctatgtc ccgtattcgg cggccgcgat cttcgcgacg acggtcgccg tcctgatcgt
     4441 cgttttctac gcctggatcc gaagcgggct caacctgcgc ttctccgatc ccagcctcac
     4501 gatcccgcag atggtcgcag ccgggctcgc gacgtcctac ctggtgtacg agagcagcat
     4561 catcgcgcgg ccggccttcg tcgcgctcta tttcatcgcc ttcatgttcg ggatcctcgc
     4621 gctcgatacg aggcggctcc tcggcgtcgc cgtcttctac gtggcctgct acgcgaccgc
     4681 ggtcgcgctc gccctgccga gccatgaggc cgaccttccg cgcgaggcga tgcgcgtcgg
     4741 catcctgtcc ctcatcctcg gcggattcag cctcatcggc ggctacgtga gccgcctgcg
     4801 gcgcaagctc aagatcgcga acacgagcct cacggaggcg ctcagcgcct cggagtcgcg
     4861 ggcgcgcatc gacgccctga ccggctgcta caaccgccgc cacgggcgcg agatcctcga
     4921 gatcgagtcg aagcgggcgg cgcgcggcga gccgctcacg atctgcctgg ccgacctgga
     4981 cggcttcaag gcgatcaacg acaagttcgg gcacgccgcc ggcgacgagg tgctcaaggg
     5041 gttcaccgtg acggtccagg ggggattgcg cgcgaccgac gcgctcgtcc gctacggcgg
     5101 cgaggagttc ctgatcgtgc tgtcgcagac gccgatcgtg gaagccgcga tggtggcgga
     5161 gcgcgtccgc cagctggtgg aggacatgag cacggtggcc ggcgcccggg gaatcaccgt
     5221 gtcgatcggg gtgaccgagc accggccgcc cgaatcggtc gatgagacga tccagcgcgc
     5281 ggactcggcg ctctaccgcg ccaaggagca gggccgcaat cgcgtcgtgt gcgcgtcggg
     5341 ggacacgagc ccggcgctcg gcgttgcctc gggcggccgc catgcctaga atgtgacgac
     5401 ctcagggcct gcgaggcgga gcggcgccgg cccgacgaag caacatccca gggagaacga
     5461 aggctcgcgg cgcgcccgtt gccgcggtcg gtgctcgcga ggagtcgtac cccttggaaa
     5521 gaaaaagaac ggcgacgcgc cgctccggcc gcgcgctcga tccggcgctg ctcgccccga
     5581 aggcgcacgg ccgcgccgag cccgtggtcg gcctcctgcc gcagccggcg cactggctca
     5641 ccggcggcgg cgagatgggc gacctgatcc gcgcgaagga ctggtcggcg acgccgctcg
     5701 gcccgctcca cgcctggccc gagagcctgc gcaccacggt ccgcatcgtc ctcgcgcagc
     5761 gcttcccgat gctgctctgg tgggggccgc agtacatcca gatctacaac gacgcctaca
     5821 tcccaattcc cggcgcgaag cacccgcagc gcgccctcgg cgtgccggtg agcgagtgct
     5881 ggtccgagat ctggcacatc ctgcggccgc tgatcgacac gccgttcaag ggcgggccgt
     5941 cgacgtggat cgaggacatc cgtctcgaca tcgaccgcca cggcttcacc gaggagacgc
     6001 acttcacgat cgcctacagt cccgtgcccg acgactcggc gcccggcggc atcggcggcg
     6061 tgctcgccac ggtgcacgag atcaccgaga aggtgatcgg cgagcggcgc atcgcgctgc
     6121 tgcgcgacct cggcgcgggc agcgccgatg ccaggaccgc cgaggacgcg tgcgacgatc
     6181 gcgtgcggcg cgcacgctct cgtcgcacgc gatcgacgtg cccttcgcgc tgatctacct
     6241 gacggacgag cacggccgcc gcgcgcgcct cgccgccacg gcgggcgtcg ccatcggcga
     6301 gccggtgagc cccggcgcga tcgaactggg cgccgccgac cagggcgaat ggccgctcgc
     6361 gcgcgcgcgc accgactcgc acatcgtcga ggtgcgcgat ctcgagacgc gcttcgcacg
     6421 ccttccgaag gattcttccg gggcgccgcc ccacaccgcg gccgtgatcc cgataccgtc
     6481 ggcgctcgcg caccggccgg ccggcttcct ggtggcggcg gtgagcccgc ggctgcgctg
     6541 ggacgagcag taccgcagct tcctgcagct cgccgcgtcg cagatcgcga cggcgatcgc
     6601 gaacgcgcgc gcctacgacg aggagcgcag gcgcgccgag gcgctggccg agatcgaccg
     6661 cgcaaagacc gccttcttct cgaacgtcag ccacgagttc cgcacgccgc tcacgctcat
     6721 gctcgggccg ctcgcggacg cgctcgcgag cccgggcggc gcgctcggcg gcgccgcgct
     6781 gcacgccgcg caccgcaacg cgcagcgcat gctcaagctc gtcaacgcgc tgctcgactt
     6841 ctcgcgcatc gaggcgggcc gcgtgcaggc ggcgttcgag ccgatcgacc tggccgcgac
     6901 caccgcggag ctcgccgggg tgttccgctc cgcggtcgag cgcgccgggc tgcgcctggt
     6961 cgtgcgctgc gagccgctcg gggagccggt gtacgtcgac accgtgatgt gggagaagat
     7021 cgtcctcaac ctgctgtcga acgccttcaa gttcaccttc gagggcgaga tcgaggtggc
     7081 gctcgcgcgc accctcgacg gcatcgagct gaaggtgcgc gacaccggca tcggcatcgc
     7141 ggcgcaggac atccccaaga tcttcgagcg cttccgccgc atcgaaggcg cgaaggcgcg
     7201 cacgcacgaa ggctcgggga tcggactgtc gctcgtgcag gagctggtgc gcatccacgg
     7261 cgggcgcatc gacgcgcaga gcgagccggg caagggcacg acgttcaccg tggcgctcaa
     7321 gcgcgggcgc ggtcatctgc ccgaggagcg cgtcggcgcg gcgcgcgagc tcgcgtccac
     7381 cgcgacgcgc gcgcaggcgt acgcggacga ggcgctcggc tggctgggcg agccagcgga
     7441 cgacgatccc gcgatctcgc cggcctcggc gcacgatccc gacgccggga tgcgcgtgct
     7501 ggtcgccgac gacaacgccg acatgcgcga gtacgtcgcg cgcctgctgc gcgcgcgctg
     7561 gacggtggca acggtcgcca acggcgaggc cgcgctcgcg gcgctgcgcc gcgagcgctt
     7621 cgacctcgtg ctcaccgacg tcatgatgcc gcggctcgac ggcttcggcc tgctgcgggc
     7681 catccgctcg gatccggcgc tcgcggcaac gcccgtggtg atgctctccg cccgcgccgg
     7741 agaggaagcg cgcatcgaag gattggacgc gggcgccgac gattacctga tcaagccctt
     7801 cggcggccgc gagctggtgg cgcgcgtgag gtcgcagctc gcgctgctcg ccgcccgccg
     7861 cgcggccgag cgcgagcgcg cggacctgct cgcgcgcgag caggcggcga agcgcgaggc
     7921 gcagctgcag cgcgagcacc tcgtgtcgct gttcatgcag gcgcccaacc cgatcgtcat
     7981 gctgcgcggg cgcgagcacg tgattcagct cgccaatccg ccggcgtgcc gcctgtgggg
     8041 gcggcagccc gcgcaggtgg tcggccgtcc gctgctcgag gcgctgcccg acctgcgcgg
     8101 ccaggtgttc aaggagctcc tcgaccgcgt gctgcacacg ggcgtcccct tcgaaggcaa
     8161 ggaggtgccc gcgaacctcg acccggcggg cgacgggtcg gtcgagaccg tgtacctgaa
     8221 tttcgtctac gcgccgatgc gcggcgtgac cggcgagatc gaaggcgtgc tggtcgtcgc
     8281 gttcgacgtc accgaccagg tgcgcgcgcg cgagcagatg acgcggctgc acgcggaggc
     8341 cgagcaggcg agccgcagca aggaccagtt cctggcgatg ctcggccacg agctgcgcgg
     8401 gccgctctcg cccatcgtca acgcgctcca gatcctgcgg ctgcgcggcg tgcagtcgcg
     8461 cgagcaggac atcgtcgagc ggcaggtcgg gcagatcacg cggctcgtcg acgacctgct
     8521 cgacgtctcg cgcatcacgc a
//
LOCUS       DATDMW010000045         9620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2002955, whole genome shotgun sequence.
ACCESSION   DATDMW010000045 DATDMW010000000
VERSION     DATDMW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002955"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VI319_01880"
     CDS             complement(<1..792)
                     /locus_tag="VI319_01880"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6862623.1"
                     /translation="MMYTCVVPGCADARHDHRAALARRPAKKKAAKPAPSTRTVRDRK
                     GRAKRVDIHCHYLNAEAAAKVAHLNPGQHEPMVRYANALTRETNVKQGQERGAKLTSI
                     EVRLRDMDRMGIDVQAICPAPQQTYYWTEPDHGREMSRMVNERLAQIVAQWPERFVAL
                     GTVPLQDVQIAVQELERGVKQLGLRGVEINPSVNGMDLTDPRLNLDAFFAKAQELDVV
                     IFMHPIGFTQGERLVDHYFNNLIGNPLETTVAASHLIFDGVIDRHP"
     gene            complement(806..1528)
                     /locus_tag="VI319_01885"
     CDS             complement(806..1528)
                     /locus_tag="VI319_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862624.1"
                     /translation="MTSATEALSLSGIDALYGESHVLHQVSFSLGAGRVLALLGRNGA
                     GKTTCMSTVIGLLPPRRGDIRLYGEPIGRLSPEAISRKGVGFVPQGRRIFPTLTVREN
                     LIVAGRSRAGTPVPWTLERVVKLFPRLEERLQQTAGSLSGGEQQMLAIGRALMGNPRV
                     LLMDEPSEGLAPLIVHEVGKTIARLKQEGQSIVLVEQNIKLALDLADDVVILNTGRIA
                     FTGGVGEVRDNEALITQHLGVF"
     gene            complement(1521..2261)
                     /locus_tag="VI319_01890"
     CDS             complement(1521..2261)
                     /locus_tag="VI319_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862625.1"
                     /translation="MTAALSIQNLKKSFGGLPATNDVSMDVTPGERRLIIGPNGAGKT
                     TLFNLITGDLRRDSGAIRLFGEEISRLAPHQRAHRGLARTYQIITLFQKDSLEHNLVL
                     SLLGLSKSRWDAFRPIARFGALNDEARRVLGLVGLGHLAKRPIGEVSYGEKRRVEIAM
                     ALAQKPKLLLLDEPLAGLSREERAAMKELIAGIPREVTVVMIEHDMDTALDLAERITV
                     LHYGKVIVEGTRAEVVADPKTREVYLGD"
     gene            complement(2272..3201)
                     /locus_tag="VI319_01895"
     CDS             complement(2272..3201)
                     /locus_tag="VI319_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6862626.1"
                     /translation="MRVAGWALLVVVLLGVPWVMGEYFVNLGSQILIAAIFAASLNLL
                     VGYGGLPSLGHAAWLGLAAYISAWMSLKLGLGHWITAPAAILGTALVACVFGWISLRA
                     TGLGFLMLTLALSQVLWGTAYRWVAVTDGDNGLRGLSRPAPFGLSLDGATAFYYFCLA
                     ITVLAIALMAMFVASPFGAALRGTRDQPRRMGALGHDVWLIRWITFVYSAFWGGVSGL
                     LFVYYNKYIHPASLSIQSSAEGLLGVIAGGSGTLAGPIVGAAIVMLLKNYVSAYIERW
                     NMLLGFVFVFIVIFMPEGVVPGLKRLWSRWRRK"
     gene            complement(3198..4274)
                     /locus_tag="VI319_01900"
     CDS             complement(3198..4274)
                     /locus_tag="VI319_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6862627.1"
                     /translation="MQYWTIQTLNSLALGGLLFLLAAGFSIIFGLMRIANLTHGSMYM
                     LGAYAGASVLTGGLRVRVPGSDLALFLVASGFLLLFGVMRLARLRQRTMVYALGAYAL
                     VAVAACALMLRTPGSAFEGAITLPDFARNMWIAVAAGALLVGVFGVALERLILRELTG
                     NALGQVLVTLGISFIVADFCLMVWGGDPIPVPTPPSLQAPLRVGSVAFPTYRLVVLVI
                     AVVTAVALYLLMDRTRVGAMIRAGVDDPQMARAVGIPVSKLFTAVFCLGAALAGAGGI
                     IGGPIMSAYPGLDADMLPLALIVVMLGGVGSLLGAFVGSFLTGAIYTFGTALLPDLAY
                     VILFLPMIFVIAFRPRGLFGRQTV"
     gene            complement(4326..5582)
                     /locus_tag="VI319_01905"
     CDS             complement(4326..5582)
                     /locus_tag="VI319_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862628.1"
                     /translation="MAFSRRRFIQATGAAAGASALGFPAIVRAQGSGGPIRLGLMTVK
                     TGPLASGGIDMERALVHYLKERNNTLVGRKVDLFVGDSGGVPAQARTKIQELVERDKI
                     QLMIGPLATAEALAADDYIRQQHLLTLSVAAADDMTQRKPNPWFTRGTSTASQCAHPM
                     ADYCYKTLGYRRMVLIADDIAYGHEMCAGFQRVFEELGGKVVQKTFPPLTVPDYGTYL
                     AQLNQKADAIYLGFAGSNGFRYLRQFNEYGLNGKIKVVGGMTALDEAVLRNMGDEALG
                     ILTACWYSAEIDNPINKKFAADFRKEWKYDPGFYAAATYTEAAVLEATLKRINGRIED
                     KEGFMKAVRGIKVDTCRGPVSFDKYGNVVGNVYIRKVARKEGRLVNEVVHTYPNVSQF
                     WTYDPDQYLKNPPYSRDYPPAKNLES"
     gene            complement(5613..6350)
                     /locus_tag="VI319_01910"
     CDS             complement(5613..6350)
                     /locus_tag="VI319_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEY6862629.1"
                     /translation="MTRVEIVDRSSLMQGLYRELDLDPKATAVVTVDMHRGHLDMDVA
                     TMPAKPEDAKRVIQNAKAALDAARSRGVPVIHVILVYRRIPGIGSEGMTSPFWRALHA
                     AQGENDRLVPGRKSTVREHNIEGSPGTQIIPELYRTGDYVIDNKKRLDCFYGTDLRQL
                     LETLGVKNVVLMGINTNTCVLNTSFTAFNYDYRVVVLSDCVASMYGDDLHVLGLQNVA
                     RCLGWVITNEQFFEKVGAASEAHALAK"
     gene            complement(6427..7290)
                     /locus_tag="VI319_01915"
     CDS             complement(6427..7290)
                     /locus_tag="VI319_01915"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6862630.1"
                     /translation="MKTTLVSIPTDSIPLDGAWYEPDGAGSRANAAGAVLLFHGNTMN
                     FYTGAPRFLPPRLAQLGFACLAFNRRGHDILAVRDSRTAEGAAFQTTREAIADNAIAA
                     RWLEARGFPDPIVIGHSNGGMLAVRHVADHPRTRALVLLSAHCGGRNMVPLASAAGLL
                     ARDRLGEITEQARKMAGEGRGRELLLLPGWWYAITAESFLDLLTEVPDVLELAPSVRC
                     PTLYLRGDREPRDLYPAEAFARASGGPCSVEIVRDCDHFYKGREAAVADIVCGWLGRT
                     LALSRADAEAP"
     gene            complement(7287..7949)
                     /gene="maiA"
                     /locus_tag="VI319_01920"
     CDS             complement(7287..7949)
                     /gene="maiA"
                     /locus_tag="VI319_01920"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY6862631.1"
                     /translation="MAMKLYGFWRSLATYRVRAALALKGVRFEETSINLMQGKQHTDE
                     YKAVNPQSVLPALVVDGGTPLFESMAILEYIEETHPKPALLPKDPAGRARVRGLAQIV
                     VADGHPLVVPRIRNYLEKEMRQDEPARNRWLAHWTSEALRAFEAHLARERETGRYCHG
                     DSPTFADICLASQMFGAKFFNVDTGAFPTASRIFAECMKLEAFDRSQPLKQPDAPANP
                     AH"
     gene            complement(7970..>9620)
                     /locus_tag="VI319_01925"
     CDS             complement(7970..>9620)
                     /locus_tag="VI319_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY6862632.1"
                     /translation="FRPDDGARHPVVLSYGPYAKGLAFQEGYPSAWQRMVAEHPDVAY
                     GSSNKYQRWEVVDPEKWVPDGYACVRVDSRGAGRSPGRIDHFSPRETKDFYDCIEWAG
                     VQPWSNGKVGLNGISYYGINQWHVASLQPPHLAAMCIWEGAADWYRDMTHHGGILSTF
                     WANWYDMKVKTVQYGLGERGPRSRVTGELVCGDETLSDSQLYENRANFGEEILAHPLD
                     DDYHQARSPVWEKVTAPFLSAANWGGQGLHPRGNFEGFVRAASKQKWLEAHGIEHWTH
                     FYTDYGVSLQKRFFARFLKGDERAWPDAPRVRLQVRHVDRFVERDEAEWPIARTAWTK
                     LYLSPAESSLTAEAPKAPASVSYAALGEGLTFLSAPLERETEITGPIAARLFLSSSTA
                     DADAFLVFRVFTADLREVVFQGAIDPHTPIAQGWLRASHRKLDPKLSTPYRPYHTHDE
                     IQPLAPGKPVQLDIEIWPTSIVVPAGHRLALSVRGRDYEWQKSTGARLSNFKNELRGC
                     GPFLHDDPRDRPAKIFGGTNTLHFGPGQEAYLLLPVVPPKR"
BASE COUNT         1511 a         3251 c         3388 g         1470 t
ORIGIN      
        1 cggatggcgg tcgatcacgc cgtcgaagat caggtggctc gccgcgaccg tggtctcgag
       61 cgggttgccg atcaggttgt tgaaatagtg gtcgaccagg cgctcgccct gggtgaagcc
      121 gatcgggtgc atgaagatga cgacgtcgag ctcctgcgcc ttggcgaaga aggcgtcgag
      181 attgagcctc gggtcggtga gatccatccc gttcacgctc gggttgatct cgacgccgcg
      241 caggccgagc tgcttcacgc cgcgctcgag ctcctgcacg gcgatctgca cgtcctgcag
      301 cggcacggtg cccagcgcga cgaagcgctc gggccactgc gcgacgatct gcgcgagccg
      361 ctcgttcacc atgcgcgaca tctcgcgtcc atggtcgggc tcggtccagt agtaggtctg
      421 ctgcggcgcc gggcagatcg cctggacatc gatgcccatg cggtccatgt ccctgaggcg
      481 cacctcgatc gaggtcagct tcgccccgcg ctcctgcccc tgcttgacgt tggtctcgcg
      541 cgtgagcgcg ttggcgtacc ggaccatcgg ctcgtgctga ccgggattga ggtgcgccac
      601 cttcgccgcc gcctccgcgt tgaggtagtg gcaatggatg tcgacgcgct tggcgcggcc
      661 tttgcggtcg cgcacggtcc tggtcgaggg cgcgggcttc gccgctttct tcttcgccgg
      721 ccggcgcgcg agcgcagccc ggtggtcgtg gcgcgcgtcg gcgcagccgg ggacgacgca
      781 ggtgtacatc atgacgaaaa gctccttaga aaactccgag atgctgggtg atcagcgctt
      841 cgttgtcgcg cacctcgccg acgccgccgg tgaacgcgat gcgccccgtg ttgaggatga
      901 cgacgtcgtc ggcgagatcg agcgcgagct tgatgttctg ctcgacgagc acgatggact
      961 ggccttcctg cttcaggcgc gcgatggtct tgccgacctc gtgcacgatc agcggcgcga
     1021 ggccctcgga gggctcgtcc atgagcagca cccgggggtt gcccatgagc gcgcggccga
     1081 tggcgagcat ctgctgctcg ccgccggaaa gcgacccggc ggtctgctgc agccgctctt
     1141 ccagccgcgg aaacagcttc accacgcgct cgagcgtcca cggaacgggc gtccccgcgc
     1201 ggctgcgccc ggcgacgatc aggttctcgc gcacggtgag cgtcgggaag atgcgccgcc
     1261 cctgaggcac gaagccgacg cctttgcgcg agatcgcctc gggcgacagg cgcccgatcg
     1321 gctcgccgta gaggcggatg tcgccgcgcc gcggcggcag caggccgatc accgtgctca
     1381 tgcacgtcgt cttgccggcg ccgttgcggc cgaggagggc gagcacgcgg cccgcgccga
     1441 gcgagaacga gacctggtga aggacgtggc tctcgccgta gagcgcgtcg atgccggaga
     1501 gcgacagggc ctcagtcgcg ctagtcaccg aggtacacct cgcgcgtctt cggatcggcc
     1561 acgacttcgg cgcgcgtccc ctcgacgatg accttgccgt agtgcagcac cgtgatgcgc
     1621 tcggcgagat cgagcgcggt gtccatgtcg tgctcgatca tgacgacggt cacctcgcgc
     1681 gggatcccgg cgatcagctc cttcatcgcg gcgcgctcct cgcgcgacaa gccggcgagc
     1741 ggctcgtcga ggagcagcag cttcggcttc tgcgcgagcg ccatcgcgat ctcgacgcgc
     1801 cgcttctcgc cgtacgagac ctcgccgatg ggccgcttcg cgagatgccc gaggcccacc
     1861 agccccagca cccggcgcgc ctcgtcgttc agcgcgccga agcgcgcgat cggccggaac
     1921 gcgtcccagc gcgacttcga caggccgagc agcgacagca ccaggttgtg ctcgagcgag
     1981 tccttctgga acagcgtgat gatctggtag gtgcgcgcca ggccgcggtg cgcgcgctgg
     2041 tgcggcgcaa gccgcgaaat ctcctcgccg aagagccgga tcgcgcccga gtcgcgccgc
     2101 aggtcgccgg tgatcaggtt gaacagcgtc gtcttcccgg cgccgttcgg accgatgatg
     2161 aggcggcgct cgcccggcgt gacgtccatc gacacgtcgt tggtcgcggg cagcccgccg
     2221 aaggatttct tcaggttctg gatcgagagc gccgcagtca cgtccactcc ctcacttgcg
     2281 cctccagcgg ctccacagcc gcttcaaccc cggcacgacg ccctcgggca tgaagatcac
     2341 gatgaacacg aacacgaagc cgagcagcat gttccagcgc tcgatgtacg ccgagacgta
     2401 gttcttgagc agcatcacga tcgccgcgcc gacgatcggg ccggcgagcg tgcccgagcc
     2461 gcccgcgatc acgccgagca ggccctcggc cgagctctgg atcgagagcg acgccgggtg
     2521 gatgtacttg ttgtaataga cgaacagcag ccccgacacg ccgccccaga aggccgagta
     2581 gacgaaagtg atccagcgga tcagccagac gtcgtgcccg agcgcgccca tgcggcgcgg
     2641 ctggtcgcgc gtgccgcgca gcgcggcgcc gaagggcgac gcgacgaaca tcgccatcag
     2701 cgcgatcgcg agcaccgtga tcgcgaggca gaagtagtag aaggccgttg cgccgtcgag
     2761 gctcaggccg aagggcgcgg ggcgcgacag cccgcgcagg ccgttgtcgc cgtcggtgac
     2821 cgcgacccag cggtacgccg tgccccagag cacctgcgac agcgcgagcg tgagcatgag
     2881 gaagccgagc ccggtcgcgc gcagcgagat ccatccgaag acgcaggcga ccagcgccgt
     2941 gccgaggatc gcggcgggcg ccgtgatcca gtgcccgagc ccgagcttga gcgacatcca
     3001 tgccgagatg taggcggcga gcccgagcca cgcggcgtgc ccgagcgagg gcagcccgcc
     3061 gtagccgacc agcaggttca ggctcgcggc gaaaatggcg gcgatcagga tctggctgcc
     3121 gaggttgacg aagtactcgc ccatcaccca cgggacgccg agcagcacga cgacgaggag
     3181 cgcccagccc gcgaccctca tacggtctgg cgcccgaaca gcccacgcgg gcgaaacgcg
     3241 atgacgaaga tcatcggcag gaacaggatg acgtacgcga gatcgggcag cagcgccgtg
     3301 ccgaaggtgt agatcgcccc ggtgaggaag ctgccgacga aggcgccgag caggctgccg
     3361 acgcccccca gcatcaccac gatgagcgcg agcggcagca tgtcggcgtc gagccccgga
     3421 tacgcggaca tgatcggacc gccgatgatc ccgcccgcgc cggcgagcgc cgccccgagg
     3481 cagaacaccg cggtgaacag cttcgagacc gggatgccga ccgcgcgcgc catctgcggg
     3541 tcgtcgacgc cggcgcggat catcgcgccc acgcgcgtgc ggtccatgag caggtacagc
     3601 gcgacggccg tgaccacggc gatcacgagg accaccagcc ggtaagtcgg gaaggccacg
     3661 ctgccgacgc gcagcggcgc ctgcagcgac ggcggcgtcg gcacggggat gggatcgccg
     3721 ccccagacca tcaggcagaa gtcggcgacg atgaacgaga tgccgagcgt gacgagaacc
     3781 tgtccgagcg cgttgcccgt gagctcgcgc aggatcagcc gctcgagcgc cacgccgaac
     3841 acgccgacca gcagcgcgcc ggccgccacc gcgatccaca tgttgcgtgc gaagtcgggg
     3901 agcgtgatgg cgccctcgaa ggccgacccg ggcgtgcgca gcatgagcgc gcacgccgcg
     3961 acggccacga gcgcgtaggc gccgagcgca tacaccatcg tccgctgccg caggcgcgcg
     4021 agacgcatga cgccgaacag cagcaggaag ccgcttgcga ccaggaacag cgccaggtcg
     4081 ctccccggca cgcgcacgcg caggccgccc gtcaggaccg aggcgccggc gtacgcgccg
     4141 agcatgtaca tcgagccgtg cgtgaggttg gcgatgcgca tcagcccgaa gatgatcgag
     4201 aagcccgcgg cgagcaggaa cagcagcccg cccagcgcga ggctgttcag agtctggatg
     4261 gtccagtact gcacgggcgg aaaaaagagg ggccggtgtg ccggccccgg gcttccttca
     4321 gtttttcacg actcgaggtt tttcgccggc gggtagtcgc gcgagtacgg cgggttcttc
     4381 aggtactggt cggggtcgta ggtccagaac tggctcacgt tggggtacgt gtgcacgacc
     4441 tcgttcacga gccgcccttc cttgcgcgcg accttccgga tgtagacgtt gccgaccacg
     4501 ttgccgtact tgtcgaacga caccgggccg cggcaggtat cgaccttgat cccgcgcacc
     4561 gccttcatga agccttcctt gtcctcgatg cgaccgttga tgcgcttcag cgtcgcctcg
     4621 agcaccgccg cctcggtgta cgtggccgcg gcgtagaagc cggggtcgta cttccattcc
     4681 ttgcggaagt cggcggcgaa tttcttgttg atcgggttgt cgatctcggc cgagtaccag
     4741 cacgcggtca ggatcccgag cgcctcgtcg cccatgttgc gcagcaccgc ctcgtcgagc
     4801 gcggtcatgc caccgacgac cttgatcttc ccgttcagcc cgtactcgtt gaactgccgc
     4861 aggtagcgga agccgttcga gccggcgaag ccgaggtaga tcgcatcggc cttctggttg
     4921 agctgcgcga ggtaggtgcc gtagtcgggc acggtgagcg gcgggaaggt cttctgcacc
     4981 accttgccgc ccagctcctc gaacacgcgc tggaagccgg cgcacatctc gtggccgtag
     5041 gcgatgtcgt cggcgatcag caccatgcgg cggtagccga gcgtcttgta gcagtagtcg
     5101 gccatgggat gcgcgcactg cgaggcggtc gaggtgccgc gcgtgaacca ggggttgggc
     5161 ttgcgctgcg tcatgtcgtc ggcggccgcg accgacagcg tgagcaggtg ctgctggcgg
     5221 atgtagtcgt cggcggcgag cgcctcggcc gtggcgagcg gcccgatcat cagctggatc
     5281 ttgtcgcgct cgaccagctc ctggatcttg gtgcgcgcct gcgccggcac gccgcccgag
     5341 tcgccgacga agagatccac cttgcggccg accagcgtgt tgttgcgctc cttgaggtag
     5401 tgcacgagcg cgcgctccat gtcgatgccg ccggaggcga ggggaccggt cttcaccgtc
     5461 atgaggccca gccggatcgg cccgccgctg ccttgcgcgc gcacgatggc cgggaagccg
     5521 agcgccgacg cgcccgccgc cgcgcccgtc gcctgaatga accgcctgcg actgaatgcc
     5581 atggtccctc ctttatgaga tggtggtgcc gatcatttcg ccagcgcgtg cgcctcggac
     5641 gccgcgccga ctttctcgaa gaactgctcg ttggtgatga cccagccgag gcagcgcgcg
     5701 acgttctgca gccccagcac gtgcaggtcg tcgccgtaca tgctcgccac gcagtcggac
     5761 agcaccacca cccggtaatc gtaattgaac gcggtgaacg aggtgttcag cacgcaggta
     5821 ttcgtgttga tgcccatcag caccacgttc ttcacgccca gcgtctcgag cagctgccgc
     5881 aggtcggtgc cgtagaagca gtcgagccgc ttcttgttgt cgatcacgta gtcgccggtg
     5941 cggtacagct ccgggatgat ctgcgtgccg ggcgagccct cgatgttgtg ctcgcgtacc
     6001 gtgctcttgc ggcccggcac caggcggtcg ttctcgcctt gagcggcgtg cagcgcgcgc
     6061 cagaagggcg acgtcatgcc ttcgctgccg atgccgggaa tgcgccggta gaccaggatg
     6121 acgtggatca cggggacgcc gcgcgagcgc gctgcgtcga gcgcggcttt tgcgttttgg
     6181 atcacgcgct tcgcgtcctc gggcttcgcg ggcatggtgg cgacgtccat gtcgaggtgg
     6241 ccgcgatgca tgtcgaccgt gaccaccgcg gtcgccttcg ggtcgaggtc gagctcccgg
     6301 tacaggccct gcatcaggct ggaacgatcg acgatctcta cccgggtcat ggagcgattc
     6361 ccttgcgggt ggggcgggag cccacgagtt tatcggaagc gccgggaatc atggaagcgg
     6421 ccccgatcac ggcgcttcgg cgtcggcgcg cgacagcgcg agggtgcgcc cgagccagcc
     6481 gcacacgata tcggcgacgg ccgcttcgcg ccccttgtag aagtgatcgc agtcgcggac
     6541 gatctcgacc gagcacggcc ccccgctcgc gcgcgcgaac gcctccgcgg gatagagatc
     6601 gcgcggctcg cggtccccgc gcagatagag cgtcgggcag cggaccgagg gcgcgagctc
     6661 gagcacgtcg ggcacctcgg tcagcaggtc gaggaagctc tcggccgtga tcgcgtacca
     6721 ccacccgggc aggagcagca gctcccgccc ccggccttcg cccgccattt tccgggcctg
     6781 ctcggtgatt tccccgaggc gatcgcgggc cagcagcccg gcggcggagg cgagggggac
     6841 catgttcctg ccgccgcaat gcgccgagag cagcaccagc gcgcgcgtgc gcgggtggtc
     6901 cgcgacgtgg cgcacggcca gcatgccgcc gttggaatgg ccgatcacga tcgggtcggg
     6961 aaagccccga gcctcgagcc agcgcgccgc gatggcgttg tcggcgatcg cctcgcgcgt
     7021 cgtctggaac gccgcccctt ccgccgttcg gctgtcgcgg accgcgagga tgtcgtgacc
     7081 cctgcggttg aacgcgaggc aggcgaagcc gagctgcgcc aggcgcggcg gcaggaagcg
     7141 cggcgcgccc gtatagaagt tcatcgtgtt gccgtggaag agcaggacgg cgccggccgc
     7201 attcgcccgc gagccggccc cgtcgggctc gtaccacgcc ccgtcgagcg ggatcgagtc
     7261 ggtcgggatc gagacgagcg tcgtcttcaa tgggccgggt tcgcgggcgc gtcgggctgc
     7321 ttgagcggct gcgagcggtc gaaggcctcg agcttcatgc actcggcgaa gatgcgcgag
     7381 gcggtcggga acgcgcccgt gtcgacgttg aagaacttcg cgccgaacat ctgcgacgcg
     7441 aggcagatgt cggcgaacgt cggcgagtcg ccgtggcagt agcggccggt ctcgcgctct
     7501 cttgcgaggt gcgcctcgaa cgcgcgcagc gcctcggagg tccagtgcgc gagccaacgg
     7561 ttgcgcgcgg gctcgtcctg gcgcatctcc ttctcgaggt agttgcggat gcgcggcacg
     7621 accagcgggt ggccgtcggc gaccacgatc tgcgcgaggc cgcgcacgcg ggcgcgcccg
     7681 gccgggtcct tcggcagcag cgcgggcttg gggtgcgtct cctcgatgta ctcgaggatc
     7741 gccatcgact cgaacagcgg cgtcccgccg tcgacgacca gcgccggcag cacgctctgc
     7801 gggttcaccg ccttgtactc gtcggtgtgc tgcttgccct gcatcaggtt gatcgaggtc
     7861 tcttcgaagc gcacgccctt cagcgcgagc gcggcgcgca cgcggtaggt cgcgagcgag
     7921 cgccagaaac cgtagagctt catcgccatg aggcctcctc caggtggaat cagcgcttcg
     7981 gcggcacgac cggcaggagc aggtacgcct cctgcccggg cccgaagtgc agggtgttgg
     8041 tcccgccgaa gatcttcgcc ggacggtcgc gcggatcgtc gtgcaggaac gggccgcagc
     8101 cgcgcagctc gttcttgaag ttcgagagcc tcgcgcccgt cgacttctgc cattcatagt
     8161 cgcgcccgcg cacgctgagc gcgagccggt gccccgccgg cacgacgatc gacgtcggcc
     8221 agatctcgat gtcgagctgc accggcttgc cgggagccag cggctggatc tcgtcgtgcg
     8281 tgtggtaagg ccggtaaggg gtcgacagct tcgggtccag cttgcggtgc gaggcgcgca
     8341 gccagccctg cgcgatcggc gtgtgcgggt cgatcgcgcc ctggaacacg acctcgcgca
     8401 gatcggcggt gaacacgcgg aacacgagga acgcgtcggc gtcggcggtc gacgaggaga
     8461 ggaagagacg cgccgcgatc ggaccggtga tctcggtctc gcgctcgagc ggcgccgaga
     8521 ggaaggtcag gccttcgccg agcgcggcgt acgagacgct cgccggcgcc ttcggcgcct
     8581 cggcggtgag cgagctctcg gcgggcgaga gatacagctt cgtccacgcg gtgcgcgcga
     8641 tcggccactc ggcctcgtcg cgctcgacga agcgatcgac gtgccggacc tgcagcctca
     8701 cgcgcggcgc atcgggccag gcgcgctcgt cgcccttgag gaagcgcgcg aagaagcgct
     8761 tctgcagcga cacgccgtag tcggtgtaga agtgcgtcca gtgctcgatg ccgtgggcct
     8821 cgagccactt ctgcttcgaa gcggcgcgga cgaagccttc gaaattgccg cggggatgca
     8881 atccctgacc accccagttc gccgccgaga ggaagggcgc cgtcaccttc tcccacaccg
     8941 gcgagcgcgc ctggtgatag tcgtcgtcga gcgggtgcgc gaggatctcc tcgccgaagt
     9001 tcgcgcggtt ctcgtagagc tgcgaatcgg aaagcgtctc gtcgccgcaa acgagctcac
     9061 ccgtgacgcg cgagcgcggc ccgcgctcgc cgaggccgta ctgcacggtc ttcaccttca
     9121 tgtcgtacca gttggcccag aacgtggaaa ggatcccgcc gtgatgggtc atgtcccggt
     9181 accagtcggc cgcgccttcc cagatgcaca tcgcggcgag atgcggcggc tgcagcgacg
     9241 cgacgtgcca ctggttgatg ccgtagtacg agatgccgtt cagcccgacc ttgccgttcg
     9301 accagggctg cacgccggcc cactcgatgc agtcgtagaa gtccttcgtc tcgcgcggcg
     9361 agaagtgatc gatgcggccg ggcgagcggc ccgcgccgcg cgagtcgacg cgcacgcagg
     9421 cgtagccgtc gggcacccat ttttccgggt cgaccacttc ccatctctgg tacttgttcg
     9481 acgagccgta ggcgacgtcg gggtgctcgg cgaccatgcg ctgccacgcc gatggatagc
     9541 cctcctggaa cgcgaggccc ttggcgtaag gaccgtagct gagcaccacg ggatggcgcg
     9601 cgccgtcgtc cgggcggaag
//
LOCUS       DATDMW010000046         2676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_633695, whole genome shotgun sequence.
ACCESSION   DATDMW010000046 DATDMW010000000
VERSION     DATDMW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633695"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VI319_01930"
     CDS             <1..395
                     /locus_tag="VI319_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY6862633.1"
                     /translation="NPALDLQLLHHRDQGHGPRSKRNHGEDASVLIDATLKENFPPIS
                     LPKREYMERARRIWEELKLPKLKPESPWYGYSLGEWSEELQQAADRAVKGDYFKTGEL
                     IARRRRKDVKMNTEVRDLEGADKGESDE"
     gene            388..1302
                     /locus_tag="VI319_01935"
     CDS             388..1302
                     /locus_tag="VI319_01935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6862634.1"
                     /translation="MSSSDDDLRRYLVEEAIEDFEEGRLSRRSALRMIAGITGATVAA
                     QVLDARGQQPALKSGYSPARSAYSVPANDASVLARYVPFQGADGGQLRGYIAHPAKQG
                     RYPVVLVCHENRGLTPHIEDVTRRVAKAGYVGMAVDLLSREGGTAMHDYETIPGLLTK
                     APPERHVQDFASGLGYAKSQPFARADRAGMTGFCFGGGITWRVAAGVPELRAAVPFYG
                     VPVQAPDVPKINAAVLALYGERDQRINANIPAIEAAMRANGKTFRKIVYKESDHAFHN
                     DTGERYNPVAAKAAWDETLDWFDKYLKA"
     gene            1320..2180
                     /locus_tag="VI319_01940"
     CDS             1320..2180
                     /locus_tag="VI319_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6862635.1"
                     /translation="MSLIGQLFRRRQSGAEPTPAPPASADGRSSIYDDLHHHQLLEQE
                     HANQYSARTILSDLFSRFRPASVLDVGCGIGTWLATARDLGVPDVFGIEGEWLDRKLA
                     KIPAERIVARDLEKGFDLGRRFDLAICLEVAEHLSADAAARFVESLAAHADVVLFSAA
                     IPFQGGHHHVNEQFPEYWAERFGHLGFLPFDFLRSAIWNNQSVLWWLRQNVLVFAKAH
                     LAGAGGPFAGLAAPGPLSIVHPEVYLSRVGSLRAVAAEHEKVMAVLSSGGSISVERGP
                     AGELKITARR"
     gene            complement(2177..>2676)
                     /locus_tag="VI319_01945"
     CDS             complement(2177..>2676)
                     /locus_tag="VI319_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6862636.1"
                     /translation="VQMNELVASIVHEQRTCARGRNIDFAVGELGVVDADASLLWHVL
                     TNLVGNAVKYTRNRDHAVVEIGRTSTPEGPVYFVKDNGAGFDPRYASKLFGVFQRLHA
                     ANEFEGTGIGLSIVRRIIERHGGRTWAEGRPGEGASFYFTLAQRASSGAARPSHAEAA
                     ETASP"
BASE COUNT          432 a          914 c          915 g          415 t
ORIGIN      
        1 cgaatcctgc gctcgacctg cagctgctgc accaccgcga ccagggccac ggcccgcgca
       61 gcaagcgcaa tcacggcgag gacgcgtcgg tcctgatcga tgcgacgctg aaggagaatt
      121 tcccgccgat ctcgcttccg aagcgcgagt acatggagcg cgcgcgcagg atctgggaag
      181 agctgaagct cccgaagctc aagcccgagt cgccctggta cggctactcg ctcggcgagt
      241 ggagcgagga gctgcagcag gccgccgacc gcgccgtgaa gggcgactac ttcaagaccg
      301 gcgagctgat cgccaggcgc cggcgcaagg acgtgaagat gaacaccgag gtgcgcgacc
      361 tcgagggggc ggacaaagga gagagcgatg agtagctcgg acgacgacct gcggcgctac
      421 ctggtcgaag aagcgatcga ggacttcgag gaaggccggc tttcgcgccg cagcgcgctc
      481 cggatgatcg ccggcatcac cggcgccacg gtcgccgcgc aggtgctcga cgcgcgcggg
      541 cagcagccgg cgctgaaatc gggctactcg ccggcgagat cggcgtacag cgtcccggcg
      601 aacgacgcat cggtactggc gcgctacgtg ccgttccagg gcgcggacgg cggccagctg
      661 cgcggctaca ttgcgcatcc ggcgaagcag ggccgctatc ccgtggtgct cgtgtgccac
      721 gagaaccgcg gcctcacgcc gcacatcgag gacgtcacgc gtcgcgtcgc gaaggccggc
      781 tacgtcggca tggcggtcga cctgctgtcg cgcgaaggcg ggacggcgat gcacgactac
      841 gagaccattc cggggcttct caccaaggcg ccgccggagc gccacgtcca ggacttcgcg
      901 agcgggctgg gctacgcgaa atcacagccg ttcgcgagag ccgaccgcgc cggcatgacg
      961 ggcttctgct tcggcggcgg catcacctgg cgcgtcgccg cgggcgtgcc cgagctgcgc
     1021 gccgcggtcc ccttctacgg cgtgccggtc caggcgcccg atgtgccgaa gatcaacgcc
     1081 gcggtgctcg cgctctacgg cgagcgcgat cagcgcatca acgccaacat acccgcgatc
     1141 gaggcggcga tgagggcgaa cgggaagacg ttccggaaga tcgtctacaa ggagtcggac
     1201 cacgccttcc acaacgacac gggcgagcgc tacaacccgg tcgccgcgaa ggccgcgtgg
     1261 gacgaaacgc tcgactggtt cgacaaatat ttgaaggcct gaggcgggtc gtccgcaaga
     1321 tgtctctcat cggccaactg ttccggcgtc gtcagtccgg cgcggaaccc accccggctc
     1381 cgcctgcctc ggccgacggg cgctcgtcga tctacgacga tctgcaccat catcagctcc
     1441 tggagcagga gcacgcgaac caatactccg cgcgcacgat cctttccgac ctgttctccc
     1501 gtttccggcc ggcgtccgtg ctcgacgtcg gatgcggtat cgggacgtgg ctcgcgaccg
     1561 cgcgcgatct tggcgtcccg gacgtcttcg gcatcgaagg cgagtggctc gaccggaagc
     1621 tcgcgaagat tcccgccgag cggatcgtcg cgcgcgatct cgagaagggc ttcgatctcg
     1681 gcaggcgctt cgacctcgcg atctgcctcg aagtggccga gcatctgagc gcagacgccg
     1741 ccgcgcgctt cgtcgagtcc ctcgccgcac acgcggatgt cgtgctgttc tcggccgcga
     1801 ttccgttcca gggcggtcat caccacgtca acgagcagtt cccggaatat tgggcggagc
     1861 gcttcggtca tctcggtttc ctgccgttcg atttcctgcg gagcgcgatc tggaacaatc
     1921 agtccgtgct ctggtggctg cgccagaacg tgctcgtatt cgcgaaggcg catctcgccg
     1981 gcgccggcgg tccgttcgcc gggctcgccg cgccgggccc gctctcgatc gtgcaccccg
     2041 aggtctacct gtcgcgtgtc ggcagcctgc gggccgtcgc cgcggagcac gagaaagtga
     2101 tggcggtcct gagctcgggc ggctcgattt cggtcgagcg cggcccggcg ggcgagctca
     2161 agatcactgc gcgccgctag ggcgaagccg tctcggcggc ttcggcatga ctcggtcggg
     2221 ccgccccgct gctcgctcgc tgcgccaagg tgaagtagaa gcttgcgcct tcgccgggcc
     2281 tgccctccgc ccaggtgcgg ccgccgtggc gctcgatgat ccggcgcacg atcgagagac
     2341 cgattcccgt gccctcgaac tcgttcgcgg cgtgcaggcg ctggaaaacg ccgaacagct
     2401 tgctcgcgta acgcggatcg aagccggcgc cgttgtcctt cacgaaatag accgggccct
     2461 ccggcgtgct cgtgcgtccg atctcgacca ccgcgtgatc gcgattgcgg gtgtacttga
     2521 cggcgttgcc cacgagattg gtcaggacgt gccagagcag gctcgcatcg gcgtcgacga
     2581 cgcccagctc gccgaccgcg aaatcgatgt tgcggccgcg cgcgcaggtg cgctgctcgt
     2641 ggacgatgct cgcgaccagc tcgttcatct gcacgg
//
LOCUS       DATDMW010000047         7268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2377758, whole genome shotgun sequence.
ACCESSION   DATDMW010000047 DATDMW010000000
VERSION     DATDMW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377758"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..1187
                     /locus_tag="VI319_01950"
     CDS             12..1187
                     /locus_tag="VI319_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY6862637.1"
                     /translation="MDASNTDPRYSLWQLVAYALKLGTLGFGGPIALVGYMHRDLVER
                     RGWIAESDYKEGLALAQLAPGPLAAQLAIYLGYVHYRVRGATLVGIAFVLPSFLMVVA
                     IGAAYVAYGGLVWMQAVFYGVGACVIGIIAMSAYKLTAKSIGRDKLLWAIYLVAAGVT
                     IATESEIVWVFLIGGVVVWLVRAPPAGWLRGGGMSAALAAQLPTASLAGSGLDWPVLT
                     QIAIFFAEAGAFVFGSGLAIVPFLYGGVVNEHHWLSERQFVDAVAVAMITPGPVVITV
                     GFIGYLVAGFPGAAVAALATFLPCYLFTIIPAPYFHKHGKKPAIVAFVDGCTAAAIGT
                     ITGAVVVLGKRSVIDAPTLVIALVTVLLVWKAKKIPEPVIVALAAIAGLIVYPLRAS"
     gene            complement(1174..1434)
                     /locus_tag="VI319_01955"
     CDS             complement(1174..1434)
                     /locus_tag="VI319_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron oxidase"
                     /protein_id="HEY6862638.1"
                     /translation="MDDRRRSRRSILKFAGLLAGASLAPGVTRDALAQQKASKQAMKY
                     QDKPNGDQKCGNCLQFEAPKSCKVVQGDVSPNGYCIAWAKKP"
     gene            1656..2402
                     /locus_tag="VI319_01960"
     CDS             1656..2402
                     /locus_tag="VI319_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862639.1"
                     /translation="MFSAFSRALCVACLCAPAAALAYRPFDSTDADVAKAGDLELELG
                     PVGRLREGSSRARVQPAIVANYGLTRDRELVLEGRREVLQDGDPDEQRSSIRDNALSL
                     KQVLREGTLQDAAGPSVATELGLLLPDVHGEKGTGASIAGIVSQRSEAATVHLNGALE
                     WTRAHEPGAFLGVILEGPHAWAVRPVAEAFGEQASGTPRTTSALVGAIWRVREDLSFD
                     IGVRRAHSGGETVRELRIGLTWAFGLRKEP"
     gene            2404..3381
                     /locus_tag="VI319_01965"
     CDS             2404..3381
                     /locus_tag="VI319_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862640.1"
                     /translation="MRKYDMILFVLASFAVTASAADLLEPAGTIELPSVKGRIDHFGV
                     DAKRHRVLVAALGNDSVEVVDVRAGRRERSLSGFGEPQGILYVPGADRIFVANGGADR
                     VDILDGASLARVRSIADMDDADNVRYDAAANRVWVGYGQGALRALDPSTGETLADIKL
                     PGHPESFQLETRRSRIFVNVPTANEVVVVDRGKGAVTARWSVPAQANFPMALDEAGRR
                     LFVGARRPAVLLVYDIDSGKPVARIPIGDDTDDLYFDAARKRVYAICGGGRVDVLRQD
                     GLDRYTLEGSVRTAPRARTGIFVADEATLYVAAPASGGAPARLLAYRVR"
     gene            3397..3684
                     /locus_tag="VI319_01970"
                     /pseudo
     CDS             3397..3684
                     /locus_tag="VI319_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            3676..4191
                     /locus_tag="VI319_01975"
     CDS             3676..4191
                     /locus_tag="VI319_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEY6862641.1"
                     /translation="MDAWSAKNGEVDRKPADAATRWVTRERPKIDRIACPWLIARFVD
                     RDAEFLYVPPKEVLDEAKRREAIPYDVPDVHFSHEGELCSFDAFIRHYRLADPALRKL
                     AVIVRGADTARLDLAPQAAGLAAVSLGLSRNFANDHEMLGHGMILYDALYAWCKEGQE
                     EIHTWNPQAYR"
     gene            4188..4811
                     /locus_tag="VI319_01980"
     CDS             4188..4811
                     /locus_tag="VI319_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862642.1"
                     /translation="MKGALVIALTVLSSAAASAETASFDRDPTGALPSGWISGVTGRG
                     NPSWKVDADASAPSTPNVLRQSGSGTFPWCVKRAVSIADGFVEVKLKPERGREDQAGG
                     VVWRWKDGDNYYVARANALENNVSLYHTTGGRRITIKYVDAPVAANAWHVLRVEFAGT
                     RIKVVFDGKSYIELTDDHIAGPGAVGVWTKADSVTAFDDFGYGALER"
     gene            4830..5735
                     /locus_tag="VI319_01985"
     CDS             4830..5735
                     /locus_tag="VI319_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEY6862643.1"
                     /translation="MLIPSSKERLLFGIACAALSLSAHVSAAGYDWSAVDKAIGRKGS
                     EMPGGVYRVGIARSDLKVTLDGVQIKPPLALGSYMAFQSMGKSGMVMGDLVLLQDEVN
                     PVMKKLIEGGIRITAIHNHLLRSEPFVMYMHYEGRGDPAKLAATIRGALEESKTPLEA
                     PASAGSPPPVELDTAAIEKTLGAKGNNNGGVLAFSIPRAETIKDAGMTVPAAAGMGIA
                     INFQPTGEGKAAITGDFVLVAKEVNPVLKTLRENGIEVTALHSHMLNDQPRLYFMHFW
                     ANDDAQKLATGLKAALDKVNRKKSA"
     gene            5769..7157
                     /locus_tag="VI319_01990"
     CDS             5769..7157
                     /locus_tag="VI319_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEY6862644.1"
                     /translation="MGYAHAIGGETFRFADLRELLAKATPLRSGDVLAGIAAASARER
                     MAARLALADVPLKRFLDEVIVPYEDDEVTRLIVDAHDAAAFAAISHLTVGAFRDWLLA
                     DTTTPETLARAAPGITPEMAAAVSKLMRNQDLILAARKCRVVTRFRDTIGLPGRLSVR
                     LQPNHPTDDPKGIAASLLDGLLYGCGDAVIGINPAGDSMPAFVKLWTMLDELIRRFEI
                     PTQSCVLTHVTNQIAAIDQGAPVDLVFQSIAGTEGANRAFGIDLALLGEARDAARSLQ
                     RGTVGDNVMYFETGQGSALSSGAHRGCDQQTLEARAYGVARAFAPLLVNTVVGFIGPE
                     YLYDGKQIVRAGLEDHFCGKLLGVPLGCDVCYTNHAEADQDDMDTLLTLLGAAGVAYV
                     MGVPGADDVMLNYQSTSFHDALYLRETLGLKRAPEFEQWLEKMQVVGRDGRLLPGARS
                     QKLLAATSELAP"
BASE COUNT         1066 a         2546 c         2543 g         1113 t
ORIGIN      
        1 ggaggggccg cttggacgca tcgaacaccg atccccgata ctcgctctgg cagctcgtcg
       61 cgtacgcgct caagctcggc acgctcggct tcggcggccc gatcgcgctg gtcggctaca
      121 tgcatcgcga cctggtcgag cgccgcggct ggatcgccga gtccgactac aaggaggggc
      181 tcgcgctggc gcagctcgcg cccgggccgc tcgcggcgca gctcgcgatc tacctcggct
      241 acgtgcacta ccgcgtccgc ggcgcgacgc tcgtgggcat cgcgttcgtg ctgccgtcgt
      301 tcctcatggt ggtcgcgatc ggtgcggcgt acgtcgcgta cggcgggctc gtgtggatgc
      361 aggccgtgtt ttacggcgtc ggcgcgtgcg tgatcggcat catcgcgatg agcgcgtaca
      421 agctgacggc gaagagcatc ggacgggaca agctgctgtg ggcgatctac ctcgtcgccg
      481 cgggcgtcac gatcgcgacc gagtcggaga tcgtctgggt ctttctgatc ggcggcgtcg
      541 tggtctggct cgtgcgtgcg ccgcccgccg gctggctccg cggcggcggg atgagcgcgg
      601 cgctcgcggc gcagctgccg acggcttcgc tcgccggatc ggggctcgac tggccggtgc
      661 tcacgcagat cgcgatcttc ttcgccgagg cgggcgcgtt cgtgttcggc agcgggctcg
      721 cgatcgttcc cttcctctac ggcggcgtgg tgaacgagca ccactggctg agcgagcgcc
      781 agttcgtcga cgcggtcgca gtggcgatga tcacgccggg gccggtcgtg atcacggtcg
      841 gcttcatcgg ctacctcgtc gccggatttc ccggcgcggc ggtggccgcg ctcgcgacct
      901 tcctgccgtg ctacctgttc acgatcatcc cggcgccgta cttccacaag cacggcaaga
      961 agcccgcgat cgtcgcgttc gtcgacggct gcacggcggc cgcgatcggg acgatcacgg
     1021 gcgcggtggt cgtgctgggg aagcgctcgg tgatcgacgc gccgacgctc gtcatcgcgc
     1081 tcgtcacggt cctcctcgtc tggaaggcga agaagatccc cgagccggtg atcgtcgcgc
     1141 tcgcggcgat cgccggcctg atcgtctatc cgctcagggc ttcttagccc aggcgatgca
     1201 gtagccgttc gggctgacgt cgccctggac gaccttgcag cttttcggcg cctcgaactg
     1261 caggcagttg ccgcacttct ggtcgccgtt gggcttgtcc tggtacttca tcgcctgctt
     1321 cgaggccttc tgctgcgcga gcgcgtcgcg cgtcacgccc ggggcgaggg atgcgccggc
     1381 cagcaggcct gcaaatttca gaatcgaacg gcgtgaacgc ctgcgatcgt ccatgggaat
     1441 catccgcggg tgagcaagcg gagcgcttgc gcaaatgtga caggcgcgcg cgcggcgcgg
     1501 ttccaggctt gcggatcctc gagttgaagt cgcccaccgc acggcctaga atcgtcgggt
     1561 cggggcgtgc agactcccgg ccttcccaag acggcatccc gtccaagttc tgcctgtcgc
     1621 tgccatagcg agggttcgtg ttggatgccg gagccatgtt ttccgcgttt tcccgggcgc
     1681 tgtgcgtcgc ctgcctttgc gcgcccgcgg ccgcgctcgc gtaccgcccg ttcgactcga
     1741 ccgacgccga cgtcgccaag gccggcgacc tggagctcga gctcggcccc gtcgggcgcc
     1801 tgcgcgaggg ctcgagccgg gcacgcgtgc agcccgcgat cgtcgcgaac tacggcctca
     1861 cgcgcgatcg ggagctggtg ctcgaaggcc ggcgcgaagt cctccaggac ggcgacccgg
     1921 acgagcagcg cagctcgatc cgggacaacg cgctctcgct caagcaggtg ctgcgcgagg
     1981 gtacgctcca ggacgcggcg ggtccgagcg tcgccaccga gctcggcctg ctccttcccg
     2041 acgtgcacgg cgagaagggg accggcgcgt cgatcgccgg catcgtctcg cagcgctccg
     2101 aggccgcgac cgtgcacctc aacggcgcgc tcgaatggac gcgcgcgcac gagcccggcg
     2161 cgttcctcgg cgtcatcctc gagggcccgc acgcgtgggc cgtgcgtccc gtcgccgagg
     2221 ccttcggcga gcaggcctcg ggcacgccgc gcacgacgtc cgcgctcgtc ggtgcgatct
     2281 ggcgcgtgcg cgaggatctc tcgttcgaca tcggcgtgcg ccgggcgcat tcgggaggcg
     2341 agacggtgcg cgagctgcgc atcgggctca cctgggcatt cggtctgaga aaggagccgt
     2401 gacatgcgca aatacgacat gatcctgttc gtcctcgctt cgttcgccgt gaccgcgtcg
     2461 gccgccgacc tgctcgagcc ggccgggacg atcgagctgc cgtcggtgaa aggacgcatc
     2521 gatcatttcg gcgtcgacgc gaagcggcac cgcgtgctcg tcgccgcgct cgggaacgac
     2581 agcgtcgagg tcgtggacgt gcgcgcgggc cgccgcgagc gcagcctctc cgggttcggc
     2641 gagccgcagg gaattctcta cgtccccggc gccgaccgga tcttcgtcgc gaacggcggc
     2701 gccgaccgcg tcgacattct cgacggggct tcgctcgcgc gcgtcaggag catcgccgac
     2761 atggacgatg cggacaacgt gcgctacgac gccgcggcga accgcgtctg ggtcggctac
     2821 ggccaaggcg cgctgcgcgc gctcgatccg tcgacgggcg agaccctcgc ggacatcaag
     2881 ctgcccgggc atcccgagtc gttccagctc gagacgcgcc gatcccggat cttcgtcaac
     2941 gtgccgaccg cgaacgaggt cgtcgtggtc gaccgcggca aaggcgccgt gaccgcgcgc
     3001 tggagcgtgc ccgcgcaggc caatttcccg atggcgctag acgaagcggg ccgacgcctc
     3061 ttcgtcggcg cgcgccgccc cgcggtgctg ctcgtctacg acatcgactc gggcaagccg
     3121 gtcgcgcgca tcccgatcgg cgacgatacc gacgacctct atttcgacgc cgcgcgcaag
     3181 cgcgtctacg cgatctgcgg cggcggccgg gtcgacgtgc tcaggcagga cggcctcgac
     3241 cgttacacgc tcgagggcag cgtgcgcacg gcgccgcgcg cgcgtaccgg gatcttcgtc
     3301 gccgacgagg cgacgctcta cgtcgccgcg ccggcctcgg gcggcgcgcc ggccaggctg
     3361 ctcgcctatc gcgtgcgcta acccatggag gcatccatga atgcatcgat ctccttttcc
     3421 gatctgcgct cggcgctcgg ctcgcgcgag ccgccgctgc tcatcgacgt gcgcaaaagc
     3481 ccggcgttcc gctcggcgac cgacacgatc gccggcgcgc ttcgccgcga tcccgacggc
     3541 gtggcatcgt gggcgaagag ccttccggct gcgggcacgg tggtggtgta ttgcgcgcgg
     3601 ggacacgagg tcagccaggg ggtcgcgcga tcgctcgccg accgcggcat cgagtcgcgc
     3661 tacctcgagg gcggcatgga cgcgtggagc gcgaagaacg gcgaggtgga ccgcaagccc
     3721 gcggacgccg cgacgcgctg ggtgacgcgc gagcggccga agatcgaccg catcgcgtgt
     3781 ccctggctga tcgcgcgctt cgtcgaccgc gacgcggagt tcctttatgt gccgcccaag
     3841 gaagtgctcg acgaggcgaa gcggcgcgag gcgattccct acgacgtccc cgacgtgcat
     3901 ttctcccacg agggcgagct ctgcagcttc gacgcgttca tccggcacta tcgcctcgcc
     3961 gatccggcgc tgcgcaagct cgccgtgatc gtgcgcggcg ccgacaccgc gcgcctggat
     4021 ctcgcgccgc aggccgccgg actcgccgcg gtgtcgctcg gcctgtcgcg caacttcgcg
     4081 aacgatcacg agatgctcgg gcacggcatg atcctctatg acgcgctcta cgcgtggtgc
     4141 aaggaagggc aggaggagat ccatacctgg aatccgcagg cgtaccgatg aaaggagccc
     4201 tcgtgatcgc tctcaccgtg ctgtcctcgg ccgccgcgtc cgcggagacc gcaagcttcg
     4261 atcgggaccc gaccggcgct cttccctccg gatggatctc gggtgtcacc gggcgcggca
     4321 atccgtcgtg gaaggtggac gccgatgcga gcgcccccag cacgcccaac gtcctgcgcc
     4381 agagcggcag cggcaccttc ccgtggtgcg tgaagcgcgc ggtctcgatc gccgacggct
     4441 tcgtcgaggt gaagctcaag cccgagcgcg ggcgcgagga ccaggcgggc ggcgtggtgt
     4501 ggcgctggaa ggacggcgac aactattacg tcgcgcgcgc caacgcgctc gagaacaacg
     4561 tgtcgctcta ccacacgacc ggcggacgcc ggatcaccat caagtatgtc gacgcgccgg
     4621 tcgcagcgaa cgcctggcac gtattgcggg tcgaattcgc gggcacccgc atcaaggtcg
     4681 tgttcgacgg caagtcttac atcgagctta cggacgacca catcgcggga cccggcgccg
     4741 tcggcgtctg gaccaaggcg gacagcgtga ccgccttcga cgacttcggc tacggcgccc
     4801 tggaacgcta acgaacccag gagatcgtca tgctgatacc aagcagcaaa gaaaggcttc
     4861 ttttcggaat cgcgtgcgcg gcgctgtcgc tctcggcgca cgtctcggcc gccggctacg
     4921 actggtcggc cgtcgacaag gccatcggcc gcaagggcag cgagatgccc ggcggcgtct
     4981 accgcgtcgg catcgcgcgc agcgacctca aggtcacgct cgacggcgtg cagatcaagc
     5041 cgccgctcgc gctcgggtcg tatatggcgt tccagagcat gggcaagagc ggcatggtga
     5101 tgggcgacct ggtgctcctg caggacgagg tgaacccggt gatgaagaag ctgatcgagg
     5161 gcggcatccg gatcaccgcg atccacaacc acctgctgcg ctccgagccc ttcgtgatgt
     5221 acatgcacta cgagggccgc ggcgatcccg ccaagctcgc cgcgacgatc cgcggcgcgc
     5281 tcgaagagag caagacgccg ctcgaggcgc ccgcgtccgc gggatcgccg ccgcccgtcg
     5341 agctcgacac cgccgcgatc gagaagacgc tcggcgccaa ggggaacaac aacggcggcg
     5401 tgctcgcttt cagcattccg cgcgccgaga cgatcaagga tgccgggatg accgtcccgg
     5461 ccgcggcggg catgggcatc gcgatcaact tccagccgac gggcgagggc aaggccgcga
     5521 tcaccggcga cttcgtgctc gtcgcgaagg aagtcaaccc ggtgctgaag acgctgcgcg
     5581 agaacggcat cgaggtcacc gcgctgcaca gccacatgct gaacgaccag ccgcgcctct
     5641 acttcatgca cttctgggcg aacgacgacg cgcagaagct cgcgaccggg ctcaaggcgg
     5701 cgctcgacaa ggtgaaccgc aagaagagcg cgtagccgcc ggtcccggga cccgtctaaa
     5761 ctggtcccat gggctacgcc cacgccatcg gcggcgagac tttccgcttc gcggacctgc
     5821 gcgagctgct cgccaaggcg acgccgctgc gctcgggcga cgtgctcgcc gggatcgcgg
     5881 cggcgtcggc gcgcgagcgc atggcggcgc ggctcgcgct ggccgacgtg cccttgaagc
     5941 gcttcctcga cgaggtgatc gttccctacg aggacgacga ggtgacgcgc ctgatcgtcg
     6001 acgctcacga cgcggcggcc ttcgcggcga tctcgcacct cacggtcggc gccttccgcg
     6061 actggctgct cgccgacacg accacgcccg agacgctcgc gcgcgccgcg cccgggatca
     6121 cgccggagat ggcggcggcg gtgtcgaagc tgatgcgcaa ccaggacctg atcctcgcgg
     6181 cgcgcaagtg ccgcgtggtg acgcgctttc gcgacaccat cgggctcccc gggcggttgt
     6241 cggtgcggct gcagccgaac catccgaccg acgatccgaa gggcatcgcc gcgtcgctgc
     6301 tcgacggact gctctacggc tgcggcgacg cggtgatcgg catcaatccc gcgggcgaca
     6361 gcatgccggc gttcgtgaag ctgtggacga tgctcgacga gctgatccgc cgcttcgaga
     6421 tcccgacgca gtcgtgcgtg ctcacccacg tgacgaacca gatcgccgcg atcgaccagg
     6481 gcgcgccggt cgacctcgtg ttccagtcga tcgcgggcac cgagggcgcg aaccgcgcgt
     6541 tcggcatcga cctcgcgctg ctcggcgagg cgcgcgacgc ggcgcgctcg ttgcagcggg
     6601 gcacggtcgg cgacaacgtg atgtatttcg agaccgggca gggcagcgcg ctctcctcgg
     6661 gcgcgcaccg cggctgcgac cagcagacgc tggaagcgcg cgcctacggc gtggcacgcg
     6721 cgttcgcgcc gctgctcgtc aacacggtcg tcggcttcat cggcccggag tatctctacg
     6781 acggcaagca gatcgtccgc gccgggctcg aggaccactt ctgcggcaag ctcctcggcg
     6841 ttccgctcgg ctgcgacgtc tgctacacga accacgccga ggccgaccag gacgacatgg
     6901 acacgctgct cacgctgctc ggcgcggcgg gcgtcgccta cgtcatgggc gtgccgggcg
     6961 ccgacgacgt gatgctcaac taccagagca cgtcgttcca cgatgcgctc tatttgcgcg
     7021 agacgctcgg cctgaagcgc gcgcccgagt tcgagcagtg gctcgagaag atgcaggtcg
     7081 tcggccgcga cgggcggctg ctcccgggcg cgcgctcgca gaagcttctc gccgcgacct
     7141 ccgagctcgc gccgtgagcc gcgaccgtcc cgtcgtgccc gatccgtgga ccgcgctgcg
     7201 cgcgctcacg cccgcgcgca tcgcgctcgg ccgcgcgggc gcgagcctgc cgaccgagga
     7261 ggtgctgc
//
LOCUS       DATDMW010000048        12032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_883436, whole genome shotgun sequence.
ACCESSION   DATDMW010000048 DATDMW010000000
VERSION     DATDMW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883436"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VI319_01995"
     CDS             complement(<1..719)
                     /locus_tag="VI319_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY6862645.1"
                     /translation="MTTEDENQIIAERRAKLAELRKSGVAFPNDFHRSHLAADLHSAW
                     GGKTNEELEPQAIPVNIAGRLMLKRVMGKASFATLQDASGRIQLYITRDAIGEDRYER
                     FKRCDLGDIVGASGMLFKTKTGELSVKVILLRPLVKSLRPLPEKFHGLTDQEQKYRQR
                     YVDLITSQESRAIFEARTRIIHEIRTYMVERGYLEVETPMMHPIPGGASARPFVTHHN
                     ALDHDFYLRVAPELYLKRLIVG"
     gene            complement(763..1092)
                     /locus_tag="VI319_02000"
     CDS             complement(763..1092)
                     /locus_tag="VI319_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY6862646.1"
                     /translation="MERGRYFVYILASKSRKLYVGVTNDLERRLFEHKHKLADGFTKQ
                     YHVDRLVHFEETTDVMSAIEREKQIKGWLREKKVALIESENPTWEDLSEAWYKTTSKA
                     DSSLRSE"
     gene            complement(1168..2272)
                     /gene="prfB"
                     /locus_tag="VI319_02005"
     CDS             complement(join(1168..2199,2201..2272))
                     /gene="prfB"
                     /locus_tag="VI319_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050326909.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY6862647.1"
                     /translation="MEAERINQIASTLADLDSRSNELRGIFDFEGKQKRLEAVEQLLA
                     DPAVWGDQKRAQELGKEKKTLDGTVTILSSITSGLRDARELFEMAREEKDDATLGGVA
                     ADVTKLERDVADLEFRRMFSDPLDPNNCFMDIQAGSGGTEAQDWAAMLERMYAKYCDH
                     KGFGMDILEESPGEVAGIKSATLKISGSYAYGHLRTETGVHRLVRKSPFDSNARRHTS
                     FASVFVYPEVDENIEVEINPAELRVDTYRASGAGGQHVNKTDSAVRITHLPTNIVVQC
                     QNDRSQHRNRAEAMALLKSKLYELELRKRQAQKEKLEEAKTDIGWGHQIRSYVLDQSR
                     IKDLRTGVEKGNTQAVLDGGLDDFIAASLKQGV"
     gene            complement(2301..2513)
                     /locus_tag="VI319_02010"
     CDS             complement(2301..2513)
                     /locus_tag="VI319_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEY6862648.1"
                     /translation="MDRKRVNSSKIRSAGYDPKSQTLELEFSDGKVVQYRGVSPEVYR
                     QFTAAPSPVSFFEDKIDESFPSTRLR"
     gene            complement(2616..4283)
                     /gene="recJ"
                     /locus_tag="VI319_02015"
                     /pseudo
     CDS             complement(2616..4283)
                     /gene="recJ"
                     /locus_tag="VI319_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
     gene            complement(4292..5254)
                     /locus_tag="VI319_02020"
     CDS             complement(4292..5254)
                     /locus_tag="VI319_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862649.1"
                     /translation="MHVQLFAPGFFDRAAASAARLPAAETLIAKGRRRTADYESPEAW
                     LCARFGVARQRDWPVAPYGLLGDGGAPGDHYWLRADPVQLIVDRDRLVLGDAVRLSRE
                     EAGSLAAALDAHFAGEMAIHPMRPERWYARLPAAPDLVTVAPSRARGASVEPNLPGGA
                     DARRFRALVNEAQMLLHAHPVNAAREARGEPPANSLWLWGGGTIAQSQPPALRQVLAD
                     DPLARGLAQAAGIAAAPLPGDAAAWLARAPGEGVVLAVPGDRAPAELERDWLAPLLAA
                     LRASRIGMVTLHLPGEASVLEVETVRADLRHFWRRRRPVAESAP"
     gene            5312..6553
                     /locus_tag="VI319_02025"
     CDS             5312..6553
                     /locus_tag="VI319_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY6862650.1"
                     /translation="MQYETIVGLRYLRAKRRNRFVSFIPLVSMLGIGLGVAALIVVLS
                     VMNGFHRELRARILGVVSHVEIQGSDGRLAGWPKIAEEASRDPRVRAAAPFVDAQALA
                     AAGARVRGTVVRGIEPALEEKVAEIGSRMVTGSLDELEPGAFRVVLGIELARALGVGR
                     GDKVALISPKGVMTPAGLVPRLKQFTVSGIFEVGTFEYDSGLALVDLGDAQRLYGLGG
                     AVSGVRLKLGDFLRAPEVARDLAGRLGPGVEVTDWTRTHASFFRAVQIEKYVMFVILL
                     LVVTVAACNVVSTLVMVVTDKQPDIAILRTLGASPRGIMRIFVVQGALIGVLGTAIGV
                     AGGVALALNVDVVEPFLERLLHFQLLPADLYYVTTLPSEVQRGDVLVITAVSLVISLL
                     ATVYPSWRASRVNPAEALRYE"
     gene            6579..7817
                     /locus_tag="VI319_02030"
     CDS             6579..7817
                     /locus_tag="VI319_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY6862651.1"
                     /translation="MPYELLVGLRYTGASRSNRFISFISLISMLGIALGVAALIVVLS
                     VMNGFETELRARILGVVAHLQITGPGGQLADWPGVAGQASRNPEVVAAAPFVDAQGMI
                     STGAEAKGALVRGIEPGLEAKVADIGTHMTAGALDELAPGSFRIVLGAELARALGVKR
                     GDRVMLMMPRGESLQSGLLPALRQLTVSGIFDLGMYEYDSTLALVNLRDAQSLYGLGD
                     RVSGVRLKLADLLRSRDVAEDLVRRIDRPLYIYDWSRSHANFFRAIQSQKSMMFLILL
                     LIVAVAAFNIVATLVMIVTDKQPDIAVLRTLGASPFSVMKVFVVQGTLIGVIGTLIGV
                     AAGIVLALNVGAITPVIERLLDVRLDPSIYYISELPSDLRHGDVAAVALVSLALSLLA
                     TLYPSWRASKLNPADALRYE"
     gene            7814..8518
                     /gene="lolD"
                     /locus_tag="VI319_02035"
     CDS             7814..8518
                     /gene="lolD"
                     /locus_tag="VI319_02035"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEY6862652.1"
                     /translation="MTPVAAENGPVLACRGLKKIFRQGEGEVPVLLGVDLEVRRGEVV
                     AIVGLSGSGKSTLLHLLGGLDAPSAGSVEVGGRPMHELSEAERGAVRNKWLGFVYQFH
                     HLLMEFTALENVAMPLLIRRVPRADALRAAGAMLGEVGLGARLSHRPGELSGGERQRA
                     ALARALVGSPACVLADEPTGNLDRRTAETVFELMLELNRAHGTSFVIVTHEPTLAARS
                     HRTLRLQDGALVPAGA"
     gene            complement(8515..9639)
                     /locus_tag="VI319_02040"
     CDS             complement(8515..9639)
                     /locus_tag="VI319_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY6862653.1"
                     /translation="MNSPDGKTRFEEEGSAPGTAARGAWARGAIVFLAVLAGIAALYL
                     GRAFLIPLCIGALASYALRPVVDALARLRVPRTLGAALVVSALTGGLVWGIASLGDNA
                     VAMLEKLPDAARKLRLAVASHDGAAQPLQNVQQAAKELQQAAKEATGGKAPARAAPPA
                     ADPWLQRYLISQSTLLVSVLAQAPVVLLLVFFLLASGEHFRSRLVRVLGMSRREANDV
                     LDEIDTQIQRYLLLILGINLAVGLLTGIAFWALGVEQPGLWGVGTAVLHFIPYLGPGV
                     IAVASAGAAYLQFGSVLKALSVAGAALAVATAIGMVVMTWLQSRIAHVNAAVVFIGLL
                     FFGWLWGAWGLLLGAPLLAIAKVICDRVGPLKPVSELLGG"
     gene            complement(9691..9840)
                     /locus_tag="VI319_02045"
     CDS             complement(9691..9840)
                     /locus_tag="VI319_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEY6862654.1"
                     /translation="MRKLLSAVVLGTFVAGLFAMSGCNTMKGAGQDIQKGGEKLENSA
                     DRNKK"
     gene            9930..10196
                     /locus_tag="VI319_02050"
     CDS             9930..10196
                     /locus_tag="VI319_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862655.1"
                     /translation="MQFQVSRRNKALSAGFGPRAARFDIAGAILTTLSRRAARWVDAP
                     FGASGSRRLDVRQLDRRSIVGKYVLAWLLGVPAFVLVIVYLLFH"
     gene            10225..10518
                     /locus_tag="VI319_02055"
     CDS             10225..10518
                     /locus_tag="VI319_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862656.1"
                     /translation="MATDLIGWASAVILMLTLGRQAWLQWKERRTRGVSRWLFAGQIA
                     ASVGFVAYSWMLSNWVFVVTNALILATALAGEAIYLRNRRLESRGRPGAGATP"
     gene            complement(10463..11023)
                     /locus_tag="VI319_02060"
     CDS             complement(10463..11023)
                     /locus_tag="VI319_02060"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY6862657.1"
                     /translation="MPRKLFRKYLPTPESIRQHRVLGRFGAWLHHPNLWHLNRRSVAG
                     GVAAGLFCGLIPGPLQMLGGAIVAVAFRVNLPVALFTTLYTNPVTIGPLYLGAYWLGR
                     LLVGGEAAPLAEAPAFSWGDMGEWLRAMAHWAVSLGKPLAVGLVALATLLAALGYALV
                     DVAWRVQVIRAWRRRRAARATRACGS"
     gene            11113..>12032
                     /locus_tag="VI319_02065"
     CDS             11113..>12032
                     /locus_tag="VI319_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY6862658.1"
                     /translation="MRSAIVAFALGVWALQRQAELPSEAVLAIAAVSAAIVLALVAHG
                     RTPRVVRAVAVASACLVLGFAWAGVRAQLRMADRLDASLEGRDVVITGVIAELPQPVE
                     RGARFDFDVESAPAGVPSRVQLSWYNGLAPEEFQEVAPVRAGERWRFTARLRRPRGNA
                     NPHGFDYEAWLLERGTGATGYVAPRGDRERLAAMVHAPSYWIERARERVRERFWDALA
                     GERFGGILIALAIGDQRAIGADDWQVFARTGVSHLMSISGLHVTMVAGIFGWLVFQLW
                     RRVERLALALPAQKAAAAAAFLGALAYCLLS"
BASE COUNT         1651 a         4188 c         4325 g         1868 t
ORIGIN      
        1 cccacgatca accgcttcag atagagctcg ggcgcgacgc ggaggtagaa gtcgtggtcg
       61 agcgcgttgt ggtgcgtcac gaacgggcgc gccgaggcgc cgcccgggat cgggtgcatc
      121 atcggcgtct cgacttcgag atagccgcgc tcgaccatgt acgtgcggat ctcgtggatg
      181 attcgcgtgc gcgcctcgaa gatcgcgcgg ctttcctggc tcgtgatgag atcgacgtag
      241 cgctggcgat acttctgctc ctggtcggtg agcccgtgga acttctcggg aagcgggcgc
      301 agcgatttca ccagcgggcg cagcagaatg actttgaccg acagctctcc ggtcttcgtc
      361 ttgaacaaca tgccgctggc gccgacgatg tcgcccaggt cgcagcgttt gaaccgctcg
      421 tatcgatcct ctccgatggc gtcgcgcgtg atgtagagct ggatgcggcc gctcgcgtcc
      481 tgcagcgtcg cgaagctcgc cttgcccatg acgcgcttga gcatcaggcg gcctgcgatg
      541 ttcacgggga tggcctgcgg ctcgagctcc tcgttcgtct tcccgcccca ggccgagtgc
      601 aggtcggccg cgaggtgact gcggtggaag tcgttcggaa aggcgacgcc tgacttacgg
      661 agctcggcca gcttcgcgcg ccgctcggcg atgatctggt tctcgtcttc tgtcgtcatt
      721 ctgaggagcg aagcgacgaa gaatctgctt ttgcttttgc cgtcattctg agcgaagcga
      781 agaatccgct tttgaagttg tcttgtacca cgcttcgctc aaatcttccc aagtcgggtt
      841 ctccgactcg atcagagcga ccttcttttc ccggagccac cccttgatct gcttctcccg
      901 ctcgatcgca gacatcacgt cggtagtttc ttcgaagtgt acgaggcgat cgacatggta
      961 ttgcttggtg aagccgtcgg ccagcttgtg cttgtgctcg aacaggcgcc gttccaggtc
     1021 gttggtcacc ccgacgtaga gcttgcgcga cttgctcgcg aggatgtaga cgaaatagcg
     1081 accgcgttcc atattcgaaa agcagattct tcgcttcgct cagaatgaca agaattagat
     1141 tcttcgcttc gctcagaatg acaaggttta gacgccctgc ttcaggctgg ccgcgatgaa
     1201 atcgtccagc ccgccgtcga gcaccgcctg cgtgttgccc ttctcgacgc cggttctcag
     1261 gtccttgatg cgcgactggt cgaggacgta cgagcggatc tggtggcccc agccgatgtc
     1321 ggtcttggct tcttccagct tctccttctg cgcctggcgc ttgcggagct cgagctcgta
     1381 gagcttcgac ttgagcagcg ccatcgcctc ggcgcggttg cggtgctgcg agcggtcatt
     1441 ctggcactgc acgacgatgt tcgtcggcag gtgcgtgatg cgcaccgccg agtcggtctt
     1501 gttcacgtgc tggccgcccg cgcccgaggc gcggtaggtg tcgacgcgca gctccgccgg
     1561 gttgatctcg acctcgatgt tctcgtcgac ctcgggatag acgaacacgc tcgcgaacga
     1621 ggtgtggcgc cgcgcgttcg agtcgaacgg cgacttgcgc acgagccggt gcacgccggt
     1681 ctcggtgcgc aggtggccgt aggcgtagga gccgctgatc ttcagcgtcg cgctcttgat
     1741 cccggcgacc tcgccgggcg actcctcgag gatgtccatg ccgaagccct tgtggtcgca
     1801 gtacttggcg tacatgcgct cgagcatcgc cgcccagtcc tgcgcctcgg tgccgcccga
     1861 gcccgcctgg atgtccatga agcagttgtt cgggtcgagc ggatcggaga acatgcgccg
     1921 gaactcgagg tcggcgacgt cgcgctcgag cttggtcacg tcggccgcga cgccgccgag
     1981 cgtggcgtcg tccttctcct cgcgcgccat ctcgaacagc tcgcgcgcgt cgcgcaggcc
     2041 cgaggtgatc gaggacagga tcgtgaccgt cccgtcgagg gtcttcttct ctttccccag
     2101 ctcctgggcc cgcttctgat cgccccagac cgcgggatcg gcgaggagct gctctacggc
     2161 ttcgaggcgc ttctgcttgc cttcgaagtc aaagatacct ccgcagctcg ttcgacctgg
     2221 aatcgaggtc ggcgagcgtc gaggcgatct ggttgatgcg ttcggcttcc atgtcggcgc
     2281 ggcaaaagcc gacattatag ctatcgaagc cgcgtcgaag gaaagctctc gtcgatcttg
     2341 tcctcgaaga agctgaccgg cgagggcgcg gcggtgaatt gccggtagac ctcgggcgac
     2401 acgccgcggt actgcacgac cttgccgtcg ctgaactcga gctcgagcgt ctgcgacttg
     2461 ggatcgtagc cggccgagcg gatcttgctg gaattgacgc gcttgcggtc catcgcggtc
     2521 tccccacgca gtcatcctga gcgcagcgaa ggatctgctt tttttacagc gttaacagca
     2581 gattcttcgc ttcgctcaga atgacagggc tcccagtgct cgaccaccag ctcgagcgcc
     2641 tgggtgccgt tgaactcgtt cacgcacacg cggtacgccg cgcgcacgcg cgcgggcagc
     2701 gggtcgagcg cgttgaagcg catcgcgtcg agcgtgcgcc cgccgcgcgc gagcttgagc
     2761 cgcaggtgct tctcgccgac gatgcgctgg cccacgacct cgaactcgtc gcagaagagc
     2821 ggctgcggga agccctggcc ccagatctgg cgctcgagca tctgcgcgag cgcgaggtcg
     2881 aggaagggcg cctcgagcga gccgtcggtc tcgatcacgt gcgcgcgctc ggccgccggc
     2941 agcagctcgc gcgcgaccgc ctcgaaggcc tcggcgaagc gcgcgaagtc cgcctcgcgc
     3001 agcgtcacgc cggccgccgc ggcgtggccg ccgaagcgcg cgagcagccc ggggtggcgc
     3061 ttcgcgacca ggtcgagcgc gtcgcgcagg tgcagtcccg ggatcgagcg gcccgagccg
     3121 cggatctcgc ccgcgctccc cggcgcgaac gcgatcgcgg gccggtggtg ccggtccttc
     3181 aggcgtcccg cgaggatgcc gaccacgccg gggtgccacg aggcgtcgaa caggcagatc
     3241 gcgaagctct cgcgcacgtc gacgcgctcg agcagcgcct cggcctggcc gcgcatctcg
     3301 gcctcgatct cgcggcgcgc ccggttcagg tcgtcgagct ggcgcgcgat cccgagcgcc
     3361 ttagcattgt cgttggtcac caggcactcg atgccgaggc tcatgtcgga gagccgcccc
     3421 gcggcgttga ggcgcggcgc gaccaggaac ccgaggtcga agccgctcgc gcgcgcggga
     3481 tcgcgcccgg cgacggcgaa cagcgcgcgc actccgggct gcgtgcgccc ggcgcggatg
     3541 cgcttgaggc cctgcgacac gagcgcgcgg ttgttcgcgt cgagcggcac gacgtcggcg
     3601 acggtgccga gcgcgaccag gtcgagcagg tcggcgaggt tgggctcggc gcgggcggcg
     3661 aaggcgccgc gcctgcgcag ctcggcgcgc agcgcgagca tcacgtagaa catgacgccg
     3721 acgccggcga gcgacttgct cgggaacgcg cacccgggct ggttcgggtt cacgatcgcg
     3781 cgcgcggcgg gcagccgctc gcccggcagg tggtgatcgg tgatcagcac ctcgatcccg
     3841 gcgcgcgcgg cggcgtcgac cgcctcgacc gcggcgatgc cgttgtcgac cgtgatgatc
     3901 aggtcgggct tgcgttgcgc cgcgagcgcg acgatctcgg gcgacaggcc gtagccgtac
     3961 tcgaagcggt tcggcaccag gtagccgacc tccgcgccga aggcggacag cgcgcgaaag
     4021 cccaccgcgc acgccgtggc gccgtcgcaa tcgtagtcgg cgacgatcag cagccgcttg
     4081 cgcgcggcga tcgcatcggc gaggagcgcg gcggccgcct cggcgtcgcg cagcagcgca
     4141 ggcggcagca gccccgcgag cgcgtcgtcg agctccaccc ggcgcgcgag cccgcgcgcc
     4201 gcgcaaaggc gcgcgagcac cgggtgcagg ccctcgcgca ccagcgcgtc gtggtgcttc
     4261 ggcggcacgg gccgggtgat catgcgcggc gtcacggagc cgattctgcc acgggccgcc
     4321 gccggcgcca gaaatgccgc aggtccgcgc gcaccgtctc cacctccagc accgaagcct
     4381 ccccgggcag gtgcagcgtc accatgccga tgcgcgacgc gcgcagcgcc gcgagcagcg
     4441 gcgcgagcca gtcgcgctcg agctcggccg gggcgcgatc gccgggcacg gcgagcacca
     4501 cgccctcgcc gggcgcacgc gcgagccagg cggccgcgtc gccggggagc ggcgccgcgg
     4561 cgatccccgc ggcctgcgcg aggccgcgcg cgagcgggtc gtcggcgagc acctggcgaa
     4621 gcgccggcgg ctgcgattgc gcgatcgtgc cgccgcccca cagccacagg ctgttcgccg
     4681 gcggctcgcc gcgcgcctcg cgcgccgcgt tcaccgggtg cgcgtgcagc agcatctgcg
     4741 cctcgttgac gagcgcgcgg aagcgtcgcg cgtcggcgcc gcccggcagg ttcggctcga
     4801 cgctcgcgcc gcgcgcgcgc gaaggcgcga cggtgaccag gtcgggcgcc gcggggagcc
     4861 gggcgtacca gcgctccggc cgcatcgggt ggatcgccat ctcgcccgcg aagtgcgcgt
     4921 cgagcgccgc ggccagcgac ccggcctcct cgcgcgagag ccggacggcg tcgccgagca
     4981 ccagccggtc gcggtcgacg atgagctgca cggggtcggc gcgcagccag tagtgatcgc
     5041 ccggcgcgcc gccgtcgccg agcaggccgt agggcgcgac cggccagtcg cgctggcgcg
     5101 cgacgccgaa gcgcgcgcac agccaggctt cgggcgactc gtaatccgcg gtccggcgcc
     5161 ggcccttcgc gatcagcgtc tcggcggcgg gaaggcgcgc ggcggacgcg gcggcgcgat
     5221 cgaagaaccc gggagcgaac agctggacgt gcatgccgcg cgagtgtaca ccgcgcctgc
     5281 ggcggcgaag cgcgtgtggc acactggcgc ggtgcaatac gaaacgatcg tcgggctgcg
     5341 ctacctgcgc gccaagcgcc gcaaccgctt cgtctccttc atcccgctcg tgtccatgct
     5401 cggcatcggg ctgggcgtcg ccgcgctcat cgtcgtgctc tcggtgatga acggcttcca
     5461 ccgcgagctg cgcgcgcgca tcctcggcgt cgtctcgcac gtcgagatcc agggatccga
     5521 cggccggctc gcgggctggc cgaagatcgc cgaggaggcg tcgcgtgacc cgcgcgtgcg
     5581 cgccgcggcg cccttcgtcg acgcgcaggc cctggccgcg gcgggcgcgc gcgtgcgcgg
     5641 gaccgtcgtg cgcggcatcg agcccgcgct cgaggagaag gtggccgaga tcggctcgcg
     5701 catggtgacg ggctcgctcg acgagctcga gccgggcgcc ttccgcgtgg tgctcggcat
     5761 cgagctcgcg cgcgcgctcg gcgtcggccg cggcgacaag gtggcgctga tcagcccgaa
     5821 gggcgtgatg acgcccgcgg gcctggtgcc gcggctgaag cagttcaccg tctcgggcat
     5881 cttcgaggtc ggcaccttcg agtacgactc ggggctcgcg ctcgtggatc tcggggacgc
     5941 gcagcgcctg tacggcctgg gcggcgcggt ctcgggcgtg cggctgaagc tcggcgactt
     6001 cctccgggcg cccgaggtgg cgcgcgacct ggccggccgc ctggggcccg gcgtcgaggt
     6061 caccgactgg acgcgcacgc acgcgagctt cttccgcgcg gtgcagatcg agaagtacgt
     6121 gatgttcgtg atcctgctgc tcgtcgtcac ggtcgcggcg tgcaacgtcg tgtcgacgct
     6181 cgtgatggtc gtcaccgaca agcagcccga catcgcgatc ctgaggacgc tcggcgcgag
     6241 cccgcgcggc atcatgagga tcttcgtggt gcagggcgcg ctgatcggcg tgctcggcac
     6301 ggcgatcggc gtcgccggcg gcgtcgcgct cgcgctcaac gtcgacgtgg tcgagccctt
     6361 cctcgagcgg ctgctccact tccagctgct gcccgcggac ctgtactacg tgacgaccct
     6421 gccctcggag gtgcagcgcg gcgacgtgct cgtgatcacc gccgtgtcgc tcgtcatctc
     6481 gctcctcgcc accgtgtacc cgagctggcg cgcgtcgcgc gtgaacccgg cggaggcgct
     6541 gcgctacgag tagcgcgcgc ccgtggcata cttcgcgcgt gccctacgag ctcctcgtcg
     6601 gcctgcgcta taccggcgcc agccgcagca atcgtttcat ctccttcatc tcgctgatct
     6661 cgatgctcgg catcgcgctc ggcgtggccg cgctcatcgt ggtgctgtcg gtgatgaacg
     6721 gcttcgagac ggagctgcgc gcgcgcatcc tcggcgtcgt ggcgcacctg cagatcaccg
     6781 gccccggcgg gcagctcgcc gactggcccg gcgtcgccgg gcaggcgtcg cgcaatcccg
     6841 aggtggtggc cgcggcgccg ttcgtggacg cgcagggcat gatctccacc ggcgccgagg
     6901 cgaagggcgc gctcgtgcgc ggcatcgagc ccgggctcga ggcgaaggtc gccgacatcg
     6961 gcacgcacat gacggccggc gcgctcgacg agctggcgcc cggatcgttc cgcatcgtgc
     7021 tcggcgccga gctcgcgcgc gcgctcggcg tgaagcgggg cgaccgggtg atgctgatga
     7081 tgccgcgcgg cgagtcgctg cagtcgggcc tgctgccggc gctgcgccag ctgaccgtgt
     7141 cgggcatctt cgacctcggc atgtacgagt acgactcgac gctcgcgctc gtgaacctgc
     7201 gcgacgcgca gagcctctac ggtctcggcg atcgcgtctc cggcgtgcgc ctgaagctcg
     7261 ccgacctgct gcgctcgcgc gacgtcgccg aggacctggt gcggcgcatc gaccggccgc
     7321 tctacatcta cgactggagc cgcagccacg cgaacttctt ccgcgcgatc cagagccaga
     7381 agagcatgat gttcctgatc ctgctgctga tcgtggcggt cgccgcgttc aacatcgtcg
     7441 cgacgctggt catgatcgtg accgacaagc agcccgacat cgcggtgctg cgcacgctcg
     7501 gcgcgagccc cttctcggtc atgaaggtgt tcgtggtgca ggggacgctg atcggcgtga
     7561 tcggcacgct gatcggcgtc gccgccggga tcgtgctcgc gctgaacgtc ggcgcgatca
     7621 cgccggtgat cgagcggctg ctcgacgtgc gcctcgatcc ctcgatctac tacatcagcg
     7681 agctgccctc ggacctgcgc cacggcgacg tcgccgcggt cgcgctggtc tcgctcgcgc
     7741 tgtcgctgct cgccacgctg tatccgagct ggcgcgcctc gaagctcaat cccgccgacg
     7801 ccttgcgcta cgaatgacgc ccgtcgccgc cgagaacggc ccggtgctcg cctgccgggg
     7861 cctgaagaag atcttccggc agggcgaggg cgaggtcccg gtgctgctcg gggtcgacct
     7921 cgaggtccgc cgcggcgagg tggtggcgat cgtgggcctg tcgggctccg gcaagagcac
     7981 gctgctgcac ctgctgggcg gcctcgacgc gccgagcgcc ggcagcgtcg aggtcggcgg
     8041 ccggccgatg cacgagctct cggaggcgga gcgcggcgcg gtgcgcaaca agtggctggg
     8101 cttcgtctac cagttccatc acctgctcat ggagttcacc gcgctcgaga acgtcgccat
     8161 gccgctcctc atccggcgcg tgccgcgcgc ggacgcgctc cgcgccgcgg gcgcgatgct
     8221 gggcgaggtc ggcctgggcg cgcggctctc gcaccggccg ggcgagctct cgggcggcga
     8281 gcgccagcgc gcggcgctcg cgcgcgcgct ggtcgggtcg cccgcgtgcg tgctcgccga
     8341 cgagccgacc ggcaacctcg accggcgcac cgccgagacg gtgttcgagc tgatgctcga
     8401 gctgaaccgc gcgcacggca cgagcttcgt catcgtcacg cacgagccga cgctcgcggc
     8461 gcgctcgcac cgcacgctgc ggctgcagga cggcgcgctc gtgcccgcgg gcgcctagcc
     8521 gccgagcagc tccgacacgg gcttgagcgg cccgacgcgg tcgcagatca ccttggcgat
     8581 cgcgagcagc ggcgccccga gcagcagccc ccacgcgccc cagagccagc cgaagaacag
     8641 cagcccgatg aacacgaccg ccgcgttgac gtgcgcgatg cggctctgca gccaggtcat
     8701 caccaccatg ccgatcgcgg tcgcgaccgc gagcgcggcg cccgcgaccg agagcgcctt
     8761 gagcaccgag ccgaactgca ggtaggcggc gccggcgctc gccacggcga tcacgcccgg
     8821 cccgagatac ggaatgaagt gcagcacggc cgtgcccacg ccccagagcc cgggctgctc
     8881 gacgccgagc gcccagaacg cgattcccgt gaggaggccg acggcgaggt tgatgccgag
     8941 gatcagcagc aggtagcgct ggatctgcgt gtcgatctcg tcgagcacgt cgttcgcctc
     9001 gcggcgcgac atgccgagga cgcgcaccag ccgcgagcgg aagtgctcgc ccgaggcgag
     9061 caggaagaag acgagcagca gcacgaccgg cgcctgcgcc agcaccgaga cgagcagcgt
     9121 cgactgcgag atcaggtagc gctgcagcca tggatcggcc gcgggcggcg cggcgcgcgc
     9181 cggggccttg ccgccggtcg cttccttcgc cgcctgctgc agctccttcg ccgcctgctg
     9241 cacgttctgc agcggctgcg cggcgccgtc gtgcgaggcg acggcgaggc ggagcttgcg
     9301 cgccgcgtcg ggaagcttct cgagcatcgc gacggcgttg tcgccgagcg acgcgatccc
     9361 ccagaccagc ccgccggtga gcgccgagac gacgagcgcc gccccgaggg tgcgcggcac
     9421 gcgcaggcgc gcgagcgcat ccacgaccgg gcgcagcgcg tagctcgcga gcgcgccgat
     9481 gcagagcggg atcaggaaag cgcggccgag atagagcgcg gcgatcccgg cgagcacggc
     9541 gaggaacacg atcgcgccgc gcgcccaggc accgcgcgcc gcggtccccg gcgcgcttcc
     9601 ttcctcctcg aatcgggttt tgccgtcggg cgagttcatc gacctcccgc ggcaaaagag
     9661 gcgagtcccc gcggatgcgg ggagccgccg ctatttctta ttgcggtccg ccgagttctc
     9721 gagcttctcg ccgcccttct ggatgtcctg gccggcaccc ttcatggtgt tgcagcccga
     9781 catcgcgaac agcccggcga cgaacgttcc cagaacgaca gcagacaaca gtttccgcat
     9841 catgatcacg actccatttc ggttgaggac agtttcctgc gcttgcacgc gccttgcttt
     9901 aggcagggca cgtgccaggc gcgtcgcaca tgcagttcca ggtttcacgg cggaacaagg
     9961 ctttgtcagc aggcttcggt ccgcgggctg caaggttcga catcgcaggc gcaattctta
    10021 caacgctgtc acgtcgagcg gcgcgttggg tggatgcgcc gttcggcgca tcgggatcgc
    10081 gccggctcga cgttagacaa ctcgatagga gatcgatcgt ggggaaatat gtgttggcgt
    10141 ggctgctggg tgtgccggct ttcgtgctgg tgatcgtcta cctgctgttc cattgatgga
    10201 cggtccgccg cgccgggggc ccgcgtggcg accgacctga tcggatgggc ctcggccgtg
    10261 atcctgatgc tgacgctcgg gcgtcaggca tggctgcagt ggaaggagcg aaggacccgg
    10321 ggggtgtcgc gctggctgtt cgccggccag atcgcggcat ccgtcgggtt cgtcgcgtac
    10381 agctggatgc tgtcgaactg ggtgttcgtg gtcacgaacg ccctcatcct cgccaccgcg
    10441 ctcgcgggcg aggcgatcta cctcaggaac cgcaggctcg agtcgcgcgg gcggcccggc
    10501 gccggcgcca cgccctgatc acctgcacgc gccaggcgac gtccaccagc gcgtagccga
    10561 gcgccgcgag cagcgtcgca agcgccacca ggccgaccgc gagcggcttg ccgagcgaca
    10621 ccgcccagtg cgccatcgcg cgcagccatt cgcccatgtc gccccacgag aacgcgggcg
    10681 cctccgcgag cggcgccgcc tcgcctccga ccagcagccg ccccagccaa tacgcgccga
    10741 ggtacagcgg cccgatcgtc accgggttcg tgtacagcgt cgtgaacagc gccaccggca
    10801 gattcacccg gaacgcgacc gccacgatcg ccccgcccag catctgcagc ggccccggga
    10861 tcagcccgca gaaaaggccg gccgccaccc cgcccgccac cgagcggcgg ttgaggtgcc
    10921 agagattggg atggtgcagc cacgcgccga agcggccgag gacccggtgc tggcggatcg
    10981 actcgggcgt ggggaggtat ttgcggaaga gcttccgggg catggcggat atctcgcgcg
    11041 gggatgttag ccgagttcaa ccgttgtcac cctcgggcgt tagagcgaaa gggccgcggc
    11101 ggagggaagg gcatgaggtc ggcgatcgtc gcctttgcgc tgggagtctg ggcgctccag
    11161 cgccaggcgg agctgccgtc ggaggcggtg cttgcgatcg ccgcggtctc ggccgcgatc
    11221 gtgctcgcgc tcgtcgcgca tggtcgcacg ccgcgtgtgg tccgcgcggt cgccgtcgca
    11281 tcggcgtgtc tcgtgctcgg cttcgcctgg gccggcgtgc gcgcgcagct tcgcatggcg
    11341 gaccgcctcg acgcgtcgct cgaaggccgc gacgtggtga tcacgggcgt gatcgccgag
    11401 ctgccgcagc cggtcgagcg cggcgcgcgc ttcgacttcg acgtggagtc ggcgccggcg
    11461 ggggtgcctt cgcgggtgca gctctcctgg tacaacgggc tcgcgccgga ggagttccag
    11521 gaggtggcgc cggtgcgcgc gggggagcgc tggcgcttta cggcgcggct gcggcgtccg
    11581 cgcggcaacg cgaacccgca cggcttcgat tacgaggcgt ggctgctcga gcgcggcacg
    11641 ggcgcgacgg gctatgtcgc gccgcgcggc gaccgcgagc ggctggccgc gatggtgcac
    11701 gcgccctcgt actggatcga gcgcgcgcgc gagcgggtgc gcgagcgctt ctgggacgcg
    11761 ctcgcgggcg agcgtttcgg cggcatcctg atcgcgctcg cgatcggcga ccagcgcgcg
    11821 atcggtgcgg acgactggca ggtgttcgcg cgcacgggcg tctcgcacct gatgagcatc
    11881 tcggggctgc acgtgaccat ggtcgccggg atcttcggct ggctcgtgtt ccagctgtgg
    11941 cggcgcgtgg agcgcctcgc gctcgcgctg ccggcgcaga aggcggccgc ggcggcggcg
    12001 ttcctcggcg cgctcgcgta ctgcctcctc tc
//
LOCUS       DATDMW010000049        27661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1008585, whole genome shotgun sequence.
ACCESSION   DATDMW010000049 DATDMW010000000
VERSION     DATDMW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 27661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27661
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008585"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..382
                     /gene="cutA"
                     /locus_tag="VI319_02070"
     CDS             65..382
                     /gene="cutA"
                     /locus_tag="VI319_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEY6862659.1"
                     /translation="MEALLVISNLPDRASAEKLAHLLIDQRLAACVNVLSPCRSVYRW
                     KGKVDDAEEFPLLIKTTRERYAALEAAVRKHHPYELPEIIALPLAAGLPAYLDWVAAE
                     TTS"
     gene            379..2244
                     /gene="dsbD"
                     /locus_tag="VI319_02075"
     CDS             379..2244
                     /gene="dsbD"
                     /locus_tag="VI319_02075"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEY6862660.1"
                     /translation="MTRKLAFLALLVLAPVLHAAPELNADGLLDPDKAFQFSARALDA
                     STIEVRYAIADGYYLYRDRFRFTVEPAAVKLGAPQFPKGIAHEDQFFGKQETYRKEVR
                     IRLPVESAVGGRLKLTVTSQGCADSGVCYVPYDQSADVVLASAGGASRAFSDLLNDEP
                     LVSRPAASPAPVANDEARFAQMLQSGSIWLTLAAFFGAGLLLTFTPCVLPMVPILSGI
                     IVGEGHAVTRARALWLSLAYVAGMAVTYTAIGVGAALSGNLLSAALQNPWVLSAFALV
                     FVALSLSMFGLYDLGLPAAIHDRLHQAHGRLQGGQWGAVAVMGMLSAAIVSPCVAAPL
                     AGALLYISQTRDTVLGGSALFAMALGMGVPLVVVGVSEGAFLPKSGHWMRTVKRFFGA
                     LLLAVAIWTIAPVVPVAVQMLLWGALLVGIGVFLHAVDPLPANAHGLVRLGKALGILA
                     LLAGVAQLAGAFSGARDPLQPLSTLIGSQPAAAQPLRFERVKTLAELETRVRGAGRPV
                     MLDFYADWCVSCKEMERFTFRAPGVQERLGRMLVLQADVTRNDADDKALLKRFRLFGP
                     PGTVFFDREGREIEGLRVIGYQAPEKFGKVLDAALAERSSVTQPSFSQRAASSAK"
     gene            complement(2202..3206)
                     /gene="hemB"
                     /locus_tag="VI319_02080"
     CDS             complement(2202..3206)
                     /gene="hemB"
                     /locus_tag="VI319_02080"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY6862661.1"
                     /translation="MQVYGSFPGVRMRRMRRDDFSRRMMREHVLTPNDFIYPVFIIDG
                     QARTESVPSMPGVSRYTVDKLMAVAEQCLALRVPTMALFPAVEPSLKTPDGKEATNPK
                     GLVPRAVAELKKRFPELGVMTDVALDPFTSHGQDGVLDASGYILNDETLAILERQALV
                     QAGAGVDIVAPSDMMDGRIGRIRAALEESKFIYTRIMAYSAKYASGFYGPFRDAVGSA
                     PSLGKGTKYTYQMDPANSDEALWEVGLDLQEGADMVMVKPGLPYLDIVRRVKDEFKAP
                     TFVYQVSGEYAMLKAAFANGWLDERVCAMEALLAFKRAGADGVLTYFALDAARWLKEG
                     "
     gene            complement(3370..4005)
                     /gene="yihA"
                     /locus_tag="VI319_02085"
     CDS             complement(3370..4005)
                     /gene="yihA"
                     /locus_tag="VI319_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY6862662.1"
                     /translation="MSPRTPAFFRAVFFKSVASLGELPESDAEIAFAGRSNAGKSSAL
                     NALTGRRLAFVSKTPGRTQLINFYRVDECSFLTDLPGYGYARVPAAARAGWEKLLGGY
                     LQTRDSLRGLALVMDARHPLTELDRRMLAWFVPTGKPVHVLLTKADKLSRSAGLQTLA
                     RVRRELTAHGPALTAQLFSSLKRSGIEEAEGVFAAWLGPHPPQIKSPGLKG"
     gene            4095..4709
                     /locus_tag="VI319_02090"
     CDS             4095..4709
                     /locus_tag="VI319_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6862663.1"
                     /translation="MLWIATLMCVSGAALAQEKADASKGQAIVSRVCAACHGPDGNSP
                     APANPKLAGQIPEYIQKQLTNFKPASGAKAERENPIMAGMAAPLSADDMRNVAAYFGQ
                     QTPKAGAAKNKDSAERGQKIWRTGDASRGVPACAACHGATGAGVPAQFPRLAGQYAEY
                     TESQLKAFRSGTRANDANRTMRTIAANLTDADISAVADYAAGLH"
     gene            4830..5327
                     /locus_tag="VI319_02095"
     CDS             4830..5327
                     /locus_tag="VI319_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6862664.1"
                     /translation="MTGHPIVEHIPRLRRYARALVGDRYAADDLVQDTLERAWNKFYL
                     WRPGSDLRAWLFAIMHNVFVNQARRRRYEVEQPLDDLPAMAVRATQSDQLELHDVDRA
                     LRTLPVEQREVVLLVVVEQLTYGEVSRALEIPIGTVMSRLSRARERLRQLLGGQPVVP
                     LKVVK"
     gene            5324..6067
                     /locus_tag="VI319_02100"
     CDS             5324..6067
                     /locus_tag="VI319_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY6862665.1"
                     /translation="MKQITESDLHAYVDGRLSSSDRAEVDGYLADHPEDAERVKTWRE
                     QASALRALYDPILEEPVPAELATIRPRPAWHRYALAASFFIVGIATGWVAKTALAPGV
                     QTPLSLPRQAAIAHVVYAPEVRHPVEVPASEEEHLVRWLSKRLGKDLTAPHLGPLGYD
                     LVGGRLLSGPQGPVAHFMYQDARGQRLTLYVSRTRGEPRDTAFRYSQEDRVSVFYWVD
                     GNFGYALSGEIGREQLLKIADVVYKQLNP"
     gene            6178..7119
                     /gene="msrP"
                     /locus_tag="VI319_02105"
     CDS             6178..7119
                     /gene="msrP"
                     /locus_tag="VI319_02105"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEY6862666.1"
                     /translation="MLIKRPQDIRPSEITPPDVYVRRRELMKAAGAAALGTLLPPSAR
                     AEGRFAGVRKSAYSTDEKAASLKEITTYNNYYEFGTDKESPAEYAKRLKTSPWTLAVE
                     GEVRRPRTYGVEDLLRFPLEERIYRLRCVEGWSLVIPWVGFALKTIIDQVQPTGKAKF
                     VEFVTAYQPDNMPGVRLRVLDWPYVEGLRMDEAMHPLTILAVGLYGDVLPNQNGAPVR
                     LVVPWKYGFKSGKSLVRIRFVENQPKTAWEKAVPSEYGFYSNVNPDVPHPRWSQATEK
                     RLGEGRFLPRIIKTQIFNGYGDQVARLYAGMDLKRYF"
     gene            7119..7778
                     /locus_tag="VI319_02110"
     CDS             7119..7778
                     /locus_tag="VI319_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012327756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEY6862667.1"
                     /translation="MPQASSAAIGPRADSGRRPLARLSPAAVSWIKAIAFALALVPLG
                     RLVAAVFLRSLGTNPVEAIIRSLGTWTLVFLCATLAVTPLRKITGWPWLLRLRRMLGL
                     YAFFYAVLHFSSYVWLDQGLDLVAILHDIPKRPFITVGFTCLLLLVPLAATSTDAMVR
                     RLGSARWRALHRLVYVVAIGGVVHFWWLVKRDVTEPAIYAVVVATLLGYRLVSALRSR
                     G"
     gene            complement(7729..10890)
                     /locus_tag="VI319_02115"
     CDS             complement(7729..10890)
                     /locus_tag="VI319_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6862668.1"
                     /translation="MNFCELFVRRPIMTVLVMMGILIFGIAGYRLLPVSTLPNVDFPT
                     IQVTAELPGASPETMASAVATVLERQFSTIAGIDEMTSVSSLGTTRITIQFALDRDID
                     AAAQDVNSAIASAARQLPATMPAPPSFRKVNPADFAIYYLALTSDAFPLSTVDEYAET
                     NLAQRISTVSGVAQVQVFGQQKYAVRVQVDPNALAARGVGINEVESAVAQANVNLPTG
                     TLYGKDRLFAVQATGQLQDAVAFRPIIVSYRNGSPIRLQELGNVVDGVQNDKVAAWFK
                     GKRGIVLAIQRQPGTNTIEVVDNIKRLLPTFRAEVPPAIEIQTLYDRSVTIRQSVHEV
                     QFTLYLALALVVMVIFIFLRNIPATIIPAIALPMSIVGTFAVMYALGYSLDNISLMAL
                     TLCVGFVVDDAIVMLENISRHIEQGKSVMQATLEGSKEIVFTIISMTLSLAAVFIPVL
                     FMGGILGRLLHEFAVTIIAAVLISGFVSLTLTPMMCSRILKPHREDAQHGRAYLFFER
                     AFDGLRDAYASTLRWTLARQRAVLVVFLGVCVLTGVLFARAPKGFLPSEDSGQLLCFV
                     EGPQDISFDAMMQIQGKVAEIIGKDPNVEAAMSFVGAGGASNSLNLGRITITLKPFNQ
                     RKPADQVVRELRPKIANVLGTKAFVNNVPAIRIGGQLTKSPYQYVVRSASVEELYHWA
                     PLIEQKLRTMPGLVDVTSDLQIKQPQVTVQIEREKASALGVSAQSIEQALNNAYGARQ
                     VSTIYTATNQYWVILELARRYQTDPSVLSMLYVRSSTGALVPLNAVARLAYGVGPLQV
                     THLGQLTSVTFSFDLRPGVALSQAIAEVQAATREMQVPGTLTGTFSGTAQAFQTSLQG
                     MGILLVLSIFVIYLVLGILYESFIHPLTILSGLPTAGLGALVTLMAFGVELNMYAFVG
                     IIMLVGIVKKNAIMMIDFAIEAQRREGMAPEEAIYRAGMVRFRPIMMTTFAALMGSFP
                     IALGLGAGGSARMPLGLAVVGGLVLSQVLTLYITPVIYLYFERLQQWLAQRRTLRSDD
                     RHRDRERLTRGSAAPRPGDTRAT"
     gene            complement(10887..12071)
                     /locus_tag="VI319_02120"
     CDS             complement(10887..12071)
                     /locus_tag="VI319_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6862669.1"
                     /translation="MDIKPPTVIGSRSNKRLLVAGVASAVGVVVLAAAALIYFGRELR
                     ANEPKGGRGPAAVPVSVATVQQQTVPVRVQAIGNVEAFSTVAVKARVDGQIVEVGFKE
                     GEEVPKGRVLFKIDPRPFDAALRQAEANHLRDVAQAEQARSQEKRYQDLLQKNFVSKE
                     AYAQIRTNADTAEAVALASRAALDNARLNVEYCTIRSPIEGYAGKIQIQMGNLVKAND
                     VGPLVTVNQVHPIYVNFSLPENRLPEVRKYMAAGPLSVNALTPSSDKVTASGTLIFID
                     NAVDPSTGTIKLRARFANKENVLWPGQFVNVSVQLFQQKDAIIIPSQAVQTGPEGQYV
                     YVVGEDMAVDLRKIQVQRSDGDNTIVASGLTKGERVVTQGQLRLGPKTRVQFGKPGPE
                     AS"
     gene            complement(12187..13440)
                     /gene="lysA"
                     /locus_tag="VI319_02125"
     CDS             complement(12187..13440)
                     /gene="lysA"
                     /locus_tag="VI319_02125"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY6862670.1"
                     /translation="MSVSFFSYHADGALYAENVPLSEVAARHGTPCYVYSRAALEAKY
                     REFAAAGAGREVDICYAVKANSNLAVLNLLARLGAGFDIVSGGELERVLAAGGAAGRT
                     VFSGVGKSEAELRSALRAGIRCFNVESEMELERLSTIAAATGEVAPVSLRVNPDVDAR
                     THPYIATGLRDTKFGVPYEDAPRLYRKAAKLPGLRVVGIDCHIGSQLTQAEPFAAALE
                     KLLEFVDMLGSTGLPLEHIDMGGGLGIRYRDEEPPSVADYVGRLLKLIGSRNLKLLLE
                     PGRALVGNAGLLLTRVEYVKHAPARNFIVVDGAMNDLVRPALYESWHEVLPVRRNAAA
                     PVDYDIVGPVCESGDFLARDRRLAAAPGDLLAIMSAGAYGMSMSSNYNTRPRAAEVMI
                     DGAAMHLVRARETVADLFARERVLP"
     gene            complement(13442..13699)
                     /locus_tag="VI319_02130"
     CDS             complement(13442..13699)
                     /locus_tag="VI319_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEY6862671.1"
                     /translation="MTVIHCIRHIIMRRVMPSRVMRPVAGLLLLVLALLPACGTKGPL
                     YLPTPEQKAQQQKKQEQRRQKQEQQQQAPQQDDNKQQPPGQ"
     gene            13699..14022
                     /gene="cyaY"
                     /locus_tag="VI319_02135"
     CDS             13699..14022
                     /gene="cyaY"
                     /locus_tag="VI319_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEY6862672.1"
                     /translation="MNDSDFDRLADETLERVSHALDASGIDCDWEIKAGGVLEIEFDD
                     GSRMIVNRHSVAQQIWVAARSGGYHFRLDGSRWVDTRDGTELFASLSRRVSEQAGTPV
                     ILRAA"
     gene            complement(14037..16337)
                     /locus_tag="VI319_02140"
     CDS             complement(14037..16337)
                     /locus_tag="VI319_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEY6862673.1"
                     /translation="MKRWLFFPVAALAGTALAGLAVLGFVLLLTYPNLPSIASLTEYQ
                     PKIPLRVYTADGVLIGEFGEERRSFVKIEEVPMIMQQAILSAEDERFYQHTGVDYLGV
                     LRAAYSNLAAGGKAQGASTITMQVARNFFLSREKTLTRKLYEALLAFKIESSLTKDQI
                     LELYINQIYLGQRAYGFGAAAQVYFGKTLAQLNVAETAMLAGLPKAPSLFNPVVNPKR
                     AKQRQLYVLRRMHELGHIDDAQYAEAQKAPLSVKKGGDSNEYAVHAEYVAEMVRQMLY
                     ERYPEDVYSRGFRVYTTITKLDQEAAYNALRNGLLEYDRRHGYRGAEAYVDLKNKDEE
                     EYEEILQDHPDSDSLLAALVVEAGPKQVKAYRRGGETVTIAGEGLRFAARMLDNSAPL
                     NRRIRRGSIIRIEKDAKDRWQIVQLPEAEAAFVSVNPQNGAVRSLVGGFDFARNQYNH
                     VTQAFRQPGSSFKPFIYSAALEKGFTPATVVNDAPVVVDAAMTGGQVWEPKNYDGKYE
                     GPMRLRTALAKSKNMVSIRVLQAIGTQYAQDYITRFGFDAERHPPYLTLALGAGSVTP
                     WQMARAYSVFATAGYRIEPYIVQKVVDDRGNVLAEAQPAQAGDESLRVIDVRNAFIMD
                     SMMHDVIRYGTGARAMSLGRYDLAGKTGTTNDYVDAWFAGYHPTLVGIAWVGFDQPKK
                     LGPSETGSVAALPIWIDYMSKALVGAPVMVMNPPDGVVSVRVNENGFQAPDGRPEYFF
                     RESVPPEQPPVEAPPENADEVKNQLF"
     gene            16676..17704
                     /locus_tag="VI319_02145"
     CDS             16676..17704
                     /locus_tag="VI319_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY6862674.1"
                     /translation="MDISSSSVKMVELVEAGKGSPKVDRYVIEALPKDAVADGNITNL
                     EAVVDTVQRGWKKLGTRTKNVALALPTAAVITKKIIVPAGLREEELELQVEGEANQYI
                     PFALDEVNLDFQVVGQAPSGPEEVEVLIAASRKEKVDDRIAVAEASGLKALIMDVEAY
                     AMQASFELIQRQFPDDGKDQNVALVDIGGNVTNITVLRNDSAVYTREQAFGGSQLTQD
                     IMRQYGMSQEEAETAKRAGGLPDNYEAEVLRPFMENAALEIQRAMQFFFTSTQYNSVE
                     HIVLTGGSAVIPGLEDMVQQRTQVSTIVANPFASMQVSSRVPLKKLVADAPSLMVACG
                     LALRRFDA"
     gene            17701..18300
                     /locus_tag="VI319_02150"
     CDS             17701..18300
                     /locus_tag="VI319_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY6862675.1"
                     /translation="MIRINLLPWRAERRKAQRKYLGTLAAMVGGLGLAIVVLVHGVIA
                     GYITIQNDRNEFLKKENARLDKEIDEIKKLKNEIAALLARKQVIERLQTDRAQAVYLL
                     QELVQQVPDGVYLKSIKQAGLRVALNGYAQSNARVSTLMRNLSASPYLENPDLVEIRA
                     VTVANKRLSEFQMNVSLRPPQQAQDAGKAPAPAAAAKKG"
     gene            18305..18931
                     /locus_tag="VI319_02155"
     CDS             18305..18931
                     /locus_tag="VI319_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY6862676.1"
                     /translation="MKQLLEQFKNLNMRDPGAWPPIPKIMLLVLILAGVLGGGYMFDW
                     QDQLKELDDGAALEVKLKDDYVNKERQAINLDLYRQQLREIDSSFGTLLKQLPNRSQM
                     DALLVDINQAGLGRGLQFELFKPAPAETKREFYAELPVTLKLNGTYHDMGHFASDIGK
                     LSRIVTLNDISLTATAGGMLTMDTVAKTFRYLDDDEIAAQRPARPGKK"
     gene            18928..19449
                     /locus_tag="VI319_02160"
     CDS             18928..19449
                     /locus_tag="VI319_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEY6862677.1"
                     /translation="MRKALALLLVLLLAACGGEEFGDLKAELKDKTKDLRGRIDPLPV
                     VKPYEPVPYKVFDQPDPFGPSKIELVTKAAPGAGGGLKPDLNRPKEPLEAYPLESLKM
                     VGVWQQPKATFALVKADTGLYRVKVGNYMGQNFGLVTAINGSSIQLRELIQDATGDWS
                     ERQSTLQLQETGR"
     gene            19523..21622
                     /gene="pilQ"
                     /locus_tag="VI319_02165"
     CDS             19523..21622
                     /gene="pilQ"
                     /locus_tag="VI319_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEY6862678.1"
                     /translation="MQAALLVLGLLAIAPLARAQNALEGFEVAHQAGDIVIKVTLKEA
                     LTTAPGSFTVANPARIAFDFPSTINALGRNNQTVGEGDLRSMNVVQAGDRTRLVLNLR
                     NMMQYDAKVDGRNVVITLTKGTAPATAQADGAAPAARFAEGRTDVTHQIKDINFRRGR
                     NGEGRIVVDLSDTSTGVDIRQQGQTLIVDFLRANLPDALRKRLDVVDFATPVQTVSAF
                     PQGDNVRLLIEPKGLWEHNAYQTDTQFVLEVKPVQYDPNKLVQGTRGGYTGEKLSLNF
                     QNVEVRSVLNVIADFTDLNIITSDSVSGNLTLRLKDVPWDQALDIILQTRGLDMRKNG
                     NVIWIAPRDELATKEKLSLESQAQITELEPVRTENFQLNYAKALDMVKILSDPNQKIL
                     SKRGNAVVDARTNQLFVQDVPSRLEEVRRLLAKMDVPVRQVLIEARIVEANDTFSKNL
                     GVRLGVHDLGGDHRLAGTNTHVDVGGGLGDTGGHAAGGAQGGTPVFIPDSLSVNVPSP
                     GLSGFNPGQLSVILFNSTFSQFLNLELSALESEGKGKIISSPRVLTADNIEALIEQGT
                     ELPYQQATSSGATAISFRKANLRLLVKPQITPDGNVIMSLDVNKDAVGQITTAGFAID
                     TKHVKTEVLVENGGTVVIGGIYTQDLRNQVVKIPFFGDLPVVGFLFRNTQRQDNKTEL
                     LVFITPRIVEDRMLGSR"
     gene            21745..22242
                     /locus_tag="VI319_02170"
     CDS             21745..22242
                     /locus_tag="VI319_02170"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY6862679.1"
                     /translation="MGAGKTTVGRALAKRLGKAFHDSDREIEARTGVRVAHIFEIEGE
                     DGFRRREAEAIERLTALDDIVLATGGGAVLDPANRDRLKSRGFVIYLHAHPRELWQRT
                     RHDRTRPLLQSADPRGRLEELYALRDPLYREVADAVVETGRQGVTLLVAQALAKIEDA
                     CRLSA"
     gene            22224..23324
                     /gene="aroB"
                     /locus_tag="VI319_02175"
     CDS             22224..23324
                     /gene="aroB"
                     /locus_tag="VI319_02175"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY6862680.1"
                     /translation="MQTVRVALGERSYPVHIGSGLLGRAELVLPQLPRKQIAVVTDTN
                     VAPLYLERFAGPLREAGVDVVTVVLPDGEAHKRWDSVERVLDTLLARRCGRDTTLAAL
                     GGGVVGDLTGFAASVYQRGVPYIQVPTTLLAQVDSSVGGKTAINHARGKNMVGAFHQP
                     ALVLADMDTLVSLPARELRAGLAEVIKHGFIRDADFVGWLEAYVDDLLARDRDALAHA
                     VARSVEIKSAIVARDEREQGERALLNFGHTFGHALETGLGYGEWLHGEAVAAGMAMAA
                     DLSRRLGILSSADCERVRALLARAGLPVEAPALPVQRLLELMSVDKKVKDGRLRFILL
                     ERIGAAVLRADVPRAVLEETLAAATPQRSLST"
     gene            23413..>27661
                     /locus_tag="VI319_02180"
     CDS             23413..>27661
                     /locus_tag="VI319_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY6862681.1"
                     /translation="MNHPALPAAQGLYDPAHEHDACGVGFVANIKGKKSHSIIEQGLT
                     VLRNLTHRGAVGWDPKLSDGAGILIQIPDKFFREELGKAGIKLPPVGQYGVGMVFLPR
                     EPASRLACEYEIERAIKDEGQVLLGWRDVPVDNSDLAEPAKRLEPVIRQVFVGRGPGV
                     TVTDALERKLYIIRKSSGHAIQALKLAHGKEFYVPSMSARTIVYKGMLLAHQVGEYYL
                     DLKDARVESALALIHQRFSTNTFPSWDLAHPFRMIAHNGEINTLRGNVNWLRARQGAI
                     SSPILGADLNKIWPLIYDGQSDSASFDNALELLVMSGYSVAHAMMMMIPEAWESHTLM
                     DPARRAFYEYHAAMMEPWDGPASIAFTDGRLIGATLDRNGLRPSRYIVTDDNLVIMGS
                     ECGCLPVPEERITKKWRLQPGKMFLVDIEKGRIIDDKELKETLASAKPYAEWIDRIRV
                     KLDEVESDKQRPERSRVPLIDRQQAFGYSQEDVKILMTPMAANGEEPVGSMGNDSPLA
                     VLSSKNKTLYHYFKQLFAQVTNPPIDPIREELVTSLVSFIGPKPNLLGVDETNPPLRL
                     EVSQPVLDFFEMEKIRHIERYTQGKFQVFELDITYPLAWGKEAIEARLASLAAQAEDA
                     VRSGHSIIIISDRKIDRDRVAIPALLALSAVHQHLVGKGLRTSTGLVVETGSAREVHH
                     FALLGGYGAEAVHPYLALETLLDLADKGALGAGVDGRKAVKNFVKAIGKGLKKVASKM
                     GISTYMSYTGAQIFEAVGLSRKLVDKYFTGTTSNVEGIGVFEVAEEAIRVHRAAFSET
                     DPVLATALDAGGEYHYRVRGEEHMWTPDAIAKLQHSVRKGEFATFKEYSRIIDDQSKR
                     HMTFRGLFEFRFDRATPVPIEEVEPASEIVKRFSTGAMSLGSISTEAHTTLAIAMNRI
                     GGKSNTGEGGEDRNRYARAKAGETLASRLGKDRVMVDIPLKEGDLLKSKIKQVASGRF
                     GVTAEYLASAEQLQIKMAQGAKPGEGGQLPGRKVSEYIAQIRYSTPGVELISPPPHHD
                     IYSIEDLAQLIHDLKNANPEASVSVKLVSEVGVGTVAAGVSKAKADHVTISGHDGGTG
                     ASPWSSIKHAGTPWELGLAETQQTLVLNRLRGRIAVQVDGQMKTGRDIVIGAMLGADE
                     FGFATAPLVAEGCIMMRKCHLNTCPVGVATQDPVLRKKFAGRPEHVINYFFFVAEEAR
                     ELMAKLGVRTFNELIGRADLLDTKKGVDHWKAKGLDFSRIFHLPQMPGDVARWNSERQ
                     NHGLERALDHRLIEIAKPALERGEKVAIDMPIRNLNRTVGTMLSHEIARRYGHDGLPD
                     DTIHIRFAGSAGQSFGAFLAKGVTLDLIGDTNDYCGKGLSGGRITVQPSPKFRGEPTQ
                     NVITGNVVLYGAIAGEAYFRGVAGERFAVRNSGAQAVV"
BASE COUNT         4673 a         9154 c         9305 g         4529 t
ORIGIN      
        1 gcgctatccc accgtcgccg acggcgtgcc gctcatggcc cgtgtccggg atgcggcctg
       61 agccgtggaa gcgctcctcg tcatctcgaa ccttcccgac cgcgcgagcg ccgagaagct
      121 cgctcacctc ctgatcgacc agcgcctcgc cgcctgcgtc aacgtcctgt cgccgtgccg
      181 ctcggtgtac cgctggaagg gtaaggtcga tgacgcggag gagttcccgc tgctcatcaa
      241 gaccacgcgc gagcgctatg ccgcgctcga agcggcggtg cgcaagcatc atccgtacga
      301 gctgcccgag atcatcgcgc tgccgctcgc ggcggggctg cccgcgtacc tcgactgggt
      361 cgcggcggag acgacgtcat gacgcgcaag ctcgcctttc tcgcattgct cgtgctcgcg
      421 ccggtcctgc acgcggcgcc cgagctgaac gccgacggcc tgctcgatcc ggacaaggcg
      481 ttccagttct ccgcgcgcgc gctcgacgcc tcgaccatcg aagtgcgcta tgcgatcgcg
      541 gacggctatt acctttaccg cgatcgcttc cgcttcaccg tcgagccggc cgcggtgaag
      601 ctgggcgcgc cgcaatttcc gaagggcatc gcccacgagg accagttctt cgggaagcag
      661 gagacctacc gcaaggaggt gcgcatccgg ctcccggtcg agagcgcggt cggcggccgt
      721 ctcaagctca cggtgacgtc gcagggatgc gcggacagcg gcgtctgcta cgtgccgtac
      781 gaccagagcg ccgacgtggt gctcgcgtcc gcgggcgggg cgtcgcgcgc gttctccgac
      841 ctgctcaacg acgagccgct agtgtcgcga ccggccgcct cgcccgcgcc ggtcgcgaac
      901 gacgaggcgc gattcgcgca gatgctccag tccggaagca tctggctgac gctcgccgcc
      961 ttcttcggcg ccgggctgct gctcacgttc acaccgtgcg tgctgccgat ggtgccgatt
     1021 ctctcgggca tcatcgtcgg ggagggtcat gccgtcacgc gcgcgcgggc gctgtggctt
     1081 tcgctcgcct acgtcgccgg catggcggtc acctacaccg cgatcggcgt cggcgccgcc
     1141 ctttcgggga acctgctgtc ggcggcgctg cagaatccct gggtgctctc ggcgttcgcg
     1201 ctcgtgtttg tcgcgctctc gctgtcgatg ttcgggctgt acgacctcgg ccttcccgcg
     1261 gcgatccacg accgcctgca ccaggcgcac ggccgcctgc agggcgggca atggggcgca
     1321 gttgccgtca tgggcatgct gtccgcggcg attgtcagcc cctgcgtcgc ggcaccgctc
     1381 gcgggcgcgc tgctctacat cagccagacg cgggacacgg tgctcggcgg cagcgcgctg
     1441 ttcgcgatgg cgctcggcat gggcgtgccg ctggtcgtgg tcggcgtctc ggagggcgcg
     1501 ttccttccga agtccgggca ctggatgcgc acggtcaagc gcttcttcgg cgcgctgctg
     1561 ctcgcggtcg caatctggac gatcgcgccg gtggtgcccg tggcggtaca gatgctgctc
     1621 tggggtgcgc tgctcgtcgg catcggggtg ttcctgcacg cggtcgaccc gctgccggcg
     1681 aacgcccacg gtctcgtgcg cctcgggaag gcgctgggca tcctggcgct gctcgcgggc
     1741 gtcgcgcagc tcgccggcgc cttctccggc gcccgcgatc cgctgcagcc gctctcgacc
     1801 ctcatcgggt cacagcccgc cgccgcccag ccgctgcgat tcgagcgcgt gaagacgctc
     1861 gccgagctcg agacccgcgt tcgcggcgcc ggccgtccgg tgatgctcga tttctacgcc
     1921 gactggtgcg tgtcgtgcaa ggagatggag cgcttcacgt tcagggcccc gggcgtgcag
     1981 gagcgcctcg gccggatgct cgtgctgcag gccgacgtga cccgcaacga cgccgacgac
     2041 aaggcgctgc tgaagcgctt ccggctgttc gggccgcccg ggaccgtctt cttcgaccgc
     2101 gaagggcgcg agatcgaggg gctgcgcgtg atcgggtacc aggcgccgga gaagttcggc
     2161 aaggtgctcg acgccgcgct cgccgagcgc tcgagcgtca ctcagccttc cttcagccag
     2221 cgcgcggcgt cgagcgcaaa gtaggtcaat acgccgtcgg cgccggcgcg cttgaatgcg
     2281 agcagcgcct ccatcgcgca cacgcgctcg tcgagccagc cgttggcgaa cgccgccttg
     2341 agcatcgcgt actcgccgct cacctggtag acgaaggtgg gcgccttgaa ctcgtccttc
     2401 acgcgccgca cgatgtcgag gtagggcagt cccggcttca ccatcaccat gtcggcgcct
     2461 tcctggagat cgaggccgac ctcccacagc gcctcgtcgg agttcgccgg atccatctga
     2521 taggtgtact tggtgccttt accgagactt ggcgcggagc cgaccgcgtc gcggaacggc
     2581 ccgtagaaac ccgaggcgta cttcgccgag tacgccatga tgcgcgtgta gatgaacttg
     2641 ctctcttcga gggcggcgcg gatccggccg atgcggccgt ccatcatgtc cgacggcgcg
     2701 acgatgtcga cgccggcgcc cgcctggacc agcgcctgcc gctcgaggat cgcgagcgtc
     2761 tcgtcgttca ggatgtagcc gctcgcgtcg aggacgccgt cctgtccgtg gctcgtgaac
     2821 ggatcgagcg cgacgtcggt catcacaccg agctccggga agcgtttctt cagctcggcg
     2881 accgcgcgcg gcacgaggcc cttggggttg gtcgcttcct tgccgtccgg cgtcttgagc
     2941 gacggctcga ccgcgggaaa cagcgccatc gtcggaacgc gcagcgcgag gcattgctcg
     3001 gcgaccgcca tgagtttgtc gaccgtgtag cgcgacactc ccggcatgga agggaccgac
     3061 tcggtacggg cctgcccgtc gatgatgaac accgggtaga tgaagtcatt cggggtgagc
     3121 acatgctcac gcatcatgcg gcgcgagaaa tcatctcgcc gcatacgccg catacgcact
     3181 ccgggaaagc ttccgtagac ctgcataatc actccacttt cgtttctcgt atcagtggtt
     3241 taacaaaaaa cacgacgatc ggggaacctt ttatttcggt tggaatccta tcgttcagac
     3301 ggcttcggcc gcctcccgct tctcctccct gagcgggtgc cttagacctt aaggcattta
     3361 gtccccgggc tatcccttta gcccggggct ttttatttgt ggcgggtgcg gccccagcca
     3421 ggcggcgaag acgccctcgg cctcctcgat gcccgaccgc ttcagactgg agaacagctg
     3481 agccgtgagc gccggcccat gcgcggtcag ctcgcgccgt acgcgcgcga gcgtctggag
     3541 gcccgctgaa cgcgacagtt tatccgcttt ggtcaggagc acgtgcaccg gctttccggt
     3601 cggcacgaac cacgcgagca tgcggcgatc gagctcggtc agcggatgcc tcgcgtccat
     3661 gaccagcgcg agaccgcgca gcgagtcgcg cgtctgcagg tacccgccga ggagcttctc
     3721 ccagccggcg cgcgcagcgg ccgggacgcg ggcgtagccg tatcccggca ggtcagtgag
     3781 gaagctgcac tcgtcgactc ggtagaagtt gatgagctgt gtgcgcccgg gcgtcttgct
     3841 cacgaacgcg aggcgccgtc cggtcagcgc gttcagcgcg ctcgatttcc cggcgttcga
     3901 gcggccggcg aacgcgatct cggcgtcgct ctcgggcagc tcgccgaggc tcgccacgct
     3961 cttgaagaag acggcgcgga agaacgccgg cgtgcgcggc gacatggcgg ggaagtgcct
     4021 aaattggcta gaatatcagc tttttccacc gaagcttcgc gacggagaac gagcgaatga
     4081 gacgcctgcc tgcaatgttg tggatcgcga cgctgatgtg cgtttccgga gcggcgctcg
     4141 cacaggagaa ggcggacgcg tcgaaaggtc aggcgattgt ctcgcgcgtg tgcgcggcct
     4201 gccatggccc ggacggcaac agccccgcgc cagcgaaccc gaagctcgcg ggccagattc
     4261 ccgagtacat ccagaagcag ctgactaatt tcaagcccgc gtccggcgcg aaggccgagc
     4321 gcgagaatcc gatcatggcg ggcatggcgg cgccgctctc ggccgacgac atgcgcaacg
     4381 tcgcggccta cttcgggcag cagacgccga aggcgggcgc ggccaagaac aaggacagcg
     4441 cggagcgcgg ccagaagatc tggcgcacgg gcgacgcgtc gcgcggggtg cctgcgtgcg
     4501 cggcttgcca cggcgcgacc ggggccggcg tgccggcaca gtttccgcgc ctcgccggcc
     4561 agtacgccga gtacaccgag agccagctga aggcatttcg ctccgggacg cgcgcgaacg
     4621 acgccaatcg cacgatgaga accatcgcgg cgaatctcac cgatgcggac atttccgccg
     4681 tcgccgacta cgccgcaggc cttcactgag acgcggacgg ggctcttgtc ccgtgaaatc
     4741 ttcctttccc gccggcgttc gctttttcgt cctggcgcgc tgcggaataa gctggcacta
     4801 atgatgttta cggtgtgacg cgctcggcga tgacgggcca cccgatcgtc gaacacatcc
     4861 cacggttgcg ccgctacgcg cgcgcactgg tcggcgaccg ctacgccgcc gacgacctgg
     4921 tgcaggacac gctggagcgc gcgtggaaca agttttactt gtggcgaccg ggaagcgatt
     4981 tgcgcgcgtg gctgttcgcg atcatgcaca acgttttcgt gaaccaggcg cggcgccggc
     5041 gctacgaggt ggagcagccg ctcgacgacc tgcctgcgat ggccgtgcga gcgacgcaat
     5101 cggatcaact ggagctgcat gacgtggaca gggcgctacg cacgctgccg gtcgagcaac
     5161 gcgaggtcgt tctcctggtc gtggtcgagc aattgaccta cggtgaggtg agccgcgccc
     5221 tcgagattcc gatcggcacg gtgatgtcgc gcctgtcgcg cgcacgcgag cggctgaggc
     5281 agctgctcgg cggtcagccc gtggtgccgc tcaaagtggt caaatgaagc agataaccga
     5341 aagcgacctg cacgcctacg tcgacggcag gctgtcgagc tccgaccgcg ccgaggtgga
     5401 cggctacctg gccgatcacc ccgaagacgc ggagcgggtg aagacctggc gcgagcaggc
     5461 gagcgccctg cgcgcgctct acgacccgat actcgaggag ccggttccgg ccgagctcgc
     5521 cacgatccgg ccgcggccgg cgtggcatcg ttatgcgctc gccgcatcgt tcttcatcgt
     5581 gggtatcgcg accggttggg tcgccaagac ggccctcgcc cccggggtgc agacgccgct
     5641 gtcgctgccc cggcaagccg cgatcgcgca cgtggtgtac gcgcccgagg tgcgtcaccc
     5701 cgtcgaggtg ccggcatccg aggaggagca cctggtgcgc tggctgtcca agcgcctcgg
     5761 caaggacctg acggcgccgc acctcggccc cctcggctac gacctggtcg gcgggcggct
     5821 gctgtccggg ccgcagggtc cggtcgcgca cttcatgtac caggacgcgc gcggacagcg
     5881 ccttacgctt tacgtgtcca ggacgcgcgg cgagccgcgc gacaccgcgt tccgctacag
     5941 ccaggaagac cgcgtgtcgg tgttctactg ggtcgacggc aatttcgggt acgcgctctc
     6001 gggagaaatc ggccgcgagc agttgttgaa gatcgctgac gtcgtctaca agcagctcaa
     6061 tccctagatt tccgtcccgg gctcgccgtg gcgtccgtcg cctttccggg cggcgccctg
     6121 taaggctttc cgcgctcgtc gcgactctgc acaatacctg ttcgttccgg agaaaccatg
     6181 ttgatcaagc gaccccagga tattcgtccc tccgagatta cgccgcccga cgtgtatgtg
     6241 cgccggcgcg agctcatgaa agccgcgggg gcggccgcgc tcggcacact gctgccgccc
     6301 tcggcgcgcg ccgagggccg attcgccggc gtgcgcaaaa gcgcctacag caccgatgag
     6361 aaggcggcga gcctcaagga aatcacgacg tacaacaatt actacgaatt cggcaccgac
     6421 aaggaatctc cggccgagta cgcgaaacgc ctcaagactt cgccctggac gctcgccgtg
     6481 gagggcgagg tacgccggcc tcggacctac ggcgtcgagg acctgctgcg cttcccgctc
     6541 gaggagcgca tctaccggct gcgctgcgtc gagggctggt cgctcgtgat cccgtgggtt
     6601 ggcttcgccc tgaagacgat catcgaccag gtgcagccga cgggcaaggc caagttcgtc
     6661 gaattcgtga ccgcttatca gccggacaac atgccgggcg tgcggctgcg cgttctcgat
     6721 tggccttacg tcgagggcct gcgcatggac gaggcgatgc atccgctcac catcctcgcg
     6781 gtcggccttt acggtgacgt cctgccgaac cagaacggcg cgccggtgcg cctggtcgtg
     6841 ccctggaaat acggcttcaa gagcggcaag tcgctggtac gcatccgctt cgtcgaaaac
     6901 cagccgaaga cggcctggga gaaggccgtg ccgtccgagt acggcttcta ctcgaacgtc
     6961 aatcccgacg tcccgcatcc gcgatggagc caggcgaccg agaagcggct cggcgagggg
     7021 cggttcctgc cgcggatcat caagacgcag atcttcaacg gttacggcga ccaggtggcg
     7081 cgactctacg ccggcatgga cctgaagcgg tatttctgat gccgcaggcg tcttcggcag
     7141 cgatcggccc gcgagccgac tcgggcaggc gaccgctcgc gcgactctcg cccgccgcag
     7201 tctcgtggat caaggcgatc gccttcgcgc tcgcgctcgt gccactcggc cgactcgtgg
     7261 ccgcagtgtt cctgcgctcg ctcggcacca atccggtcga agcgatcatc cgctcgctcg
     7321 ggacgtggac gctggttttt ctttgcgcca cgcttgccgt gacgccgctc cggaaaatca
     7381 ccggttggcc ctggctgctg cgcctgcggc gcatgctcgg cctgtacgcc ttcttctatg
     7441 ccgtgctgca tttttcgagc tacgtctggc tggaccaggg cctggatttg gtcgcgatcc
     7501 tgcacgacat accgaagcgg cccttcatca cggtcggctt cacctgcctg ctgctgctcg
     7561 tgccgctcgc cgctacctcg accgacgcga tggtgcggcg cctggggtcc gcgcgctgga
     7621 gagccctgca ccggctcgtc tacgtcgtcg cgatcggcgg cgtcgtgcac ttctggtggc
     7681 tggtgaagcg cgacgtcacc gagcccgcga tctacgccgt cgtggtggct acgttgctcg
     7741 ggtatcgcct ggtctcggcg ctgcggagcc gcgggtgagc cgctcgcggt cgcgatgccg
     7801 gtcgtccgac cgcagggtgc gccgctgcgc gagccactgc tggagccgct cgaagtagag
     7861 atagatcacg ggcgtgatgt agagcgtgag cacctgcgag agcaccaggc cgccgacgac
     7921 cgcgaggccg agcggcatgc gcgcgctgcc gcccgcgccc aggccgagcg cgatgggaaa
     7981 gctgcccatg agggccgcga aggttgtcat catgatcggc cggaagcgca ccatgccggc
     8041 gcggtagatc gcttcctcgg gcgccatgcc ctcgcgccgt tgcgcctcga tcgcgaagtc
     8101 gatcatcatg atcgcgttct tcttcacgat gccgaccagc atgatgatgc cgacgaaggc
     8161 gtacatgttc agctcgacgc cgaatgccat cagcgtcacc agcgcgccga ggcccgccgt
     8221 cggcagcccc gagaggatcg tgagcgggtg gatgaagctc tcgtagagga ttccgagcac
     8281 gaggtagatc acgaagatgg acagcacgag caggatgccc atgccctgca gcgaggtctg
     8341 gaacgcctgc gccgtgcccg agaacgtccc cgtgagcgtc cccggaacct gcatctcgcg
     8401 cgtggcggcc tggacctcgg cgatcgcctg cgacagcgca acgccgggac gaagatcgaa
     8461 cgagaacgtg accgaggtca gctggccgag gtgcgtgacc tgcagcggtc cgacaccgta
     8521 ggcgagcctc gcgacggcgt tcagcgggac gagcgcgccc gtcgaggagc gcacatacag
     8581 catcgacagg accgagggat cggtctggta gcgccgggcg agctcgagga tgacccagta
     8641 ctggttggtt gccgtgtaga tggtcgagac ctggcgcgcg ccgtaggcgt tgttgagcgc
     8701 ctgctcgatc gattgcgccg acacgccgag cgcggacgcc ttctcgcgct cgatctggac
     8761 cgtcacctgc ggctgcttga tctgcagatc gctggtgacg tcgaccagcc cgggcatcgt
     8821 gcgcagcttc tgctcgatga gcggcgccca gtggtacagc tcctcgacgc tggcgctgcg
     8881 cacgacgtac tggtacgggc tcttggtgag ctgcccgccg atgcggatcg ccggaacgtt
     8941 gttgacgaac gccttggtgc cgaggacgtt cgcgatcttc ggacgcagct cgcgcacgac
     9001 ctggtcggcc ggcttgcgct ggttgaacgg tttcagcgtg atcgtgatgc gaccgaggtt
     9061 cagtgaattg gaagcaccgc ccgcgcctac gaacgacatg gcggcttcga cgttcggatc
     9121 cttgccgatg atctcggcca ccttgccctg gatctgcatc atggcgtcga acgagatgtc
     9181 ctgcggcccc tcgacgaagc agagcagctg tccggagtct tcgctgggga ggaagccttt
     9241 cggcgcccgg gcgaacagca cgccggtcag cacgcagacg ccgagaaaca ccacgagcac
     9301 cgctcgctgc cgcgcgagtg tccagcgcag ggtgctggcg taggcgtcgc gaagcccgtc
     9361 gaacgcgcgc tcgaagaaga ggtaggcgcg gccgtgctgc gcatcctcgc gatgcggctt
     9421 caggatccgg ctgcacatca tcggggtcag cgtgagcgag acgaagcccg agatcaggac
     9481 cgccgcgatg atggtcaccg cgaactcgtg cagcagccgc cccaggatgc cgcccatgaa
     9541 gagcaccggg atgaacacgg ccgccaggga cagcgtcatc gagatgatcg tgaagacgat
     9601 ttccttcgag ccctcgagcg tcgcctgcat gacgctcttg ccctgctcga tgtggcggct
     9661 gatgttctcg agcatcacga tcgcgtcgtc gaccacaaag ccgacgcaca gcgtgagcgc
     9721 catcagcgag atgttgtcca ggctgtagcc gagcgcgtac atcacggcga acgtgcccac
     9781 gatcgacatc ggcagcgcga tcgccgggat gatggttgcc gggatgttgc gcaggaagat
     9841 gaagatgacc atgacgacga gcgcgagcgc gaggtacagc gtgaactgca cctcgtggac
     9901 cgactgtcgt atcgtcaccg agcggtcgta gagcgtctgg atctcgatcg ccggcggcac
     9961 ctcggcgcgg aaggtcggca gcagccgctt gatgttgtcc accacctcga tcgtgttcgt
    10021 gccgggctgg cgctggatcg cgagcacgat gccgcgcttg cccttgaacc aggccgctac
    10081 tttgtcgttc tgcacgccgt cgacgacgtt gccgagctcc tggagtcgga tgggcgagcc
    10141 gttgcggtag ctgacgatga tcggccggaa cgccaccgcg tcctgcagct gcccggtggc
    10201 ctgcaccgcg aacaggcgat ccttgccgta gagcgttccg gtcggcaggt tcacgttcgc
    10261 ctgcgccaca gccgactcga cctcgttgat gccgacgccg cgcgcggcga gcgcgttggg
    10321 atcgacctgg acccgcacgg catatttctg ctgaccgaag acctgcacct gcgccacacc
    10381 gctcacggtc gagatgcgct gcgcgaggtt ggtctcggcg tactcgtcca cggtcgacag
    10441 cgggaacgcg tcggacgtga gggcgaggta gtagatcgcg aagtcggccg ggttgacctt
    10501 gcggaacgac ggcggcgccg gcatggtcgc gggaagctgg cgcgccgccg aagcgatcgc
    10561 cgagttcacg tcctgggcgg cggcgtcgat gtcgcggtcg agcgcgaact ggatcgtgat
    10621 ccgggtcgtg ccgaggctgc tgaccgaggt catctcgtcg atgccggcga tggtcgagaa
    10681 ctgacgctcg agcacggtcg ccaccgcgga tgccatggtc tccgggctcg cgcccggcag
    10741 ctcggcagtg acctggatgg tgggaaagtc gacgttgggc agcgtcgaga ccggcagcag
    10801 ccggtagccc gcgatgccga agatcaggat ccccatcatg accagcacgg tcatgatggg
    10861 acgccgtacg aagagctcgc agaaattcac gaggcttcgg ggccgggctt cccgaattga
    10921 actcgcgtct tcgggcccag gcgaagctgc ccttgagtta cgacgcgctc acccttcgtc
    10981 agtcctgagg cgacgatggt gttatcgccg tcgctgcgct ggacctggat cttgcgcaga
    11041 tcgaccgcca tgtcctcgcc cacgacatag acgtactggc cctcggggcc ggtctgcacc
    11101 gcctgggacg ggataatgat ggcgtccttc tgctggaaca gctgcacgct gacgttgacg
    11161 aactgaccgg gccagaggac gttctccttg ttcgcgaagc gcgcgcgcag cttgatggtc
    11221 cccgtgctcg ggtcgaccgc gttgtcgatg aaaatgaggg tgccgctggc ggtcaccttg
    11281 tccgacgagg gcgtgagcgc attcaccgag agcggtccgg cggccatgta cttgcgcacc
    11341 tccggcagcc ggttctccgg gagcgagaag ttgacgtaga tcggatggac ctggttgacg
    11401 gtcacgagcg gaccgacgtc gttcgccttc accaggttgc ccatctggat ctggatcttt
    11461 ccggcgtagc cctcgatcgg cgagcggatg gtgcagtact cgacgttgag gcgggcgttg
    11521 tcgagcgcgg cgcggctcgc gagcgcgacc gcctcggcgg tgtcggcgtt ggtgcggatc
    11581 tgcgcgtacg cctccttgga gacgaagttc ttctgaagca ggtcctggta gcgcttctcc
    11641 tgcgagcgcg cctgctcggc ctgcgcgacg tcgcgcaggt gattggcctc ggcctggcgc
    11701 agcgccgcgt cgaacggccg cgggtcgatc ttgaacagca cgcgtccttt cggcacctcc
    11761 tcgccttcct tgaatccgac ctcgacgatc tggccgtcga cgcgcgcctt caccgccacg
    11821 gtggagaaag cctcgacgtt gccgatcgcc tgcacccgga cgggcaccgt ctgctgctgc
    11881 accgtagcga ccgagaccgg cacggcggcg gggccgcggc cgcccttcgg ctcgttcgcg
    11941 cgcagctcgc ggccgaaata gatcagcgcc gccgctgcga gcacgacgac gccgaccgcg
    12001 ctcgcgacgc cggcaaccag cagccgcttg ttgctccgcg acccgatcac cgtcggaggt
    12061 ttgatgtcca cggcccggct aacgcgcgca ccgcgcggcg tattccggcg ccgcggaccg
    12121 cggctcggaa tgtaaagatt tgtgaatcaa acgcgacgtt ctccgtgcac ggaacagcgt
    12181 tccaatttac ggcaaaaccc gttctctggc aaagagatcg gcgacggttt cgcgggcgcg
    12241 cacgaggtgc atcgccgcgc catcgatcat cacctccgcg gcgcgcggac gcgtgttgta
    12301 gttggagctc atgctcatgc cgtaagcgcc cgcggacatg atggcgagca gatcacccgg
    12361 cgccgccgcg agccggcgat cacgcgcgag gaagtcgccc gactcgcaca cggggccgac
    12421 gatgtcgtaa tcgacgggcg ccgccgcgtt cctgcgcacc ggcagcacct cgtgccacga
    12481 ctcgtacagc gcaggtcgga ccaggtcgtt catcgcgccg tcgacgacga tgaagttcct
    12541 cgccggcgca tgcttcacgt attcgacgcg cgtgagcagc agccccgcgt ttccgaccag
    12601 cgcgcgcccg ggctcgagca ggagcttcag gttgcgcgag ccaatcagct tcaggagccg
    12661 gcccacgtag tccgcgaccg agggaggctc ctcgtcgcga tagcggatgc cgaggccgcc
    12721 gcccatgtcg atgtgttcga gcggaagccc ggtcgagccc agcatgtcga cgaattcgag
    12781 cagcttctcg agcgcggcgg cgaaaggctc ggcctgcgtg agctgagagc cgatgtggca
    12841 gtcgatcccg acgacgcgca ggccgggcag cttcgccgcc ttgcggtaaa gccgcggtgc
    12901 gtcctcgtac gggacgccga acttggtgtc gcgaaggccc gtggcgatgt acggatgcgt
    12961 ccgggcgtcg acgtcggggt tgaccctcag cgataccggc gcgacttctc ccgtggcggc
    13021 agcgatcgtc gagagacgct cgagctccat ctcggactcg acgttgaagc agcggattcc
    13081 ggcgcgcagc gccgagcgca gctcggcctc gctcttgccc acgccggaga acaccgtcct
    13141 gcccgccgct ccgccggctg cgagcacccg ctcgagctcg ccgcccgaca cgatgtcgaa
    13201 accagcgccc aggcgcgcga gcaggttcag caccgccagg ttggagttgg ccttgaccgc
    13261 gtagcagatg tccacctcgc gaccggcgcc cgcggcggcg aactcgcggt acttggcctc
    13321 gagcgccgcg cgcgagtaga cgtagcaggg agtgccgtgc ctcgcggcga cttcggaaag
    13381 cggcacattc tcggcgtaca gggcgccgtc ggcgtgatac gaaaaaaaag acacggacag
    13441 gttactggcc aggcggttgt tgtttattat catcctgctg cggcgcctgc tgctgttgtt
    13501 cttgtttctg tcgccgctgc tcctgctttt tctgctgctg ggctttctgc tcgggggtgg
    13561 gaaggtagag cgggcccttg gtcccgcagg ccggcaggag cgcgagcacc aggaggagca
    13621 ggcctgcaac tggacgcatg acccggctag gcatgacccg gcgcatgatg atatggcgga
    13681 tgcagtgtat cacggtcaat gaacgactcc gatttcgacc gccttgccga cgagacgctg
    13741 gagcgcgtat cgcacgcgct cgatgcgagc ggcatcgatt gcgactggga gatcaaggcg
    13801 ggcggcgtgc tcgagatcga gttcgacgac ggcagccgca tgatcgtcaa ccggcattcc
    13861 gtcgcgcagc agatctgggt cgctgcgcgc tcggggggat accacttccg gctcgacggc
    13921 tcgcgctggg tcgacacgcg ggacggcacc gagctgttcg ccagcctttc gcgccgcgtg
    13981 agcgagcagg cgggtacccc cgtcatcctg cgcgccgcct gagtgcggcg cccgcgctag
    14041 aacagctggt tcttcacctc gtcggcgttc tcgggcggcg cctcgactgg cggctgctcg
    14101 ggcggaacgc tctcacggaa gaagtactcc ggacgcccgt cgggcgcctg gaagccgttc
    14161 tcgttgaccc gcacgctgac cacgccgtcg ggcggattca tgaccatcac gggcgcgccg
    14221 acgagcgcct tcgacatgta atcgatccag atggggagcg cggcgacgga cccggtctcg
    14281 ctggggccga gcttcttcgg ctgatcgaaa cccacccagg caatgccgac cagggtcggg
    14341 tgatagcccg cgaaccaggc gtcgacgtag tcattggtgg tgccggtctt gccggcgaga
    14401 tcgtagcggc ccagcgacat ggcgcgcgca ccggtgccgt agcggatcac gtcgtgcatc
    14461 atgctgtcca tgatgaaggc attgcgcacg tcgatcacgc gcaggctctc gtcgcccgcc
    14521 tgcgcgggct gcgcctcggc cagcacgttg ccgcggtcgt cgaccacctt ctgaacgatg
    14581 tagggctcga tgcggtagcc cgccgtcgcg aaaaccgaat acgcgcgcgc catctgccag
    14641 ggcgtgaccg agccggcgcc cagagcgagg gtaaggtaag gcggatggcg ctcggcgtcg
    14701 aacccgaagc gcgtgatgta gtcttgcgcg tactgcgtgc cgatcgcctg cagcacccgg
    14761 atcgagacca tgttcttgga tttcgcgagc gccgtgcgca ggcgcatcgg accctcgtac
    14821 ttgccgtcgt agttcttggg ctcccacacc tgtccgccgg tcatggcggc gtcgaccacg
    14881 acgggcgcat cgttgacgac ggtggccggc gtgaacccct tctcgagcgc ggccgagtag
    14941 atgaacggct tgaacgacga gccgggctgc cggaacgcct gggtgacgtg gttgtactgg
    15001 ttgcgggcga agtcgaagcc gccgaccagc gagcgtaccg cgccgttctg ggggttcacc
    15061 gagacgaacg ccgcttcggc ctcgggaagc tgcacgatct gccagcggtc cttcgcgtcc
    15121 ttctcgatcc ggatgatgga tccgcgtcgg atcctgcggt tgagcggagc gctgttgtcg
    15181 agcatgcgcg cggcaaaacg cagaccctcg ccggcaatgg tcacagtttc gccgccgcgg
    15241 cggtacgcct tcacctgctt ggggcctgct tcgaccacga gggcggcgag caggctgtcg
    15301 ctgtcgggat ggtcctgcag gatctcctcg tattcttcct cgtccttgtt cttcaggtcg
    15361 acgtaggcct cggcgccgcg gtagccgtgg cggcggtcgt actcgagcag gccgttgcgc
    15421 agcgcgttgt acgcggcctc ctggtcgagc ttcgtgatcg tggtgtagac gcggaagccg
    15481 cgcgagtaga cgtcttcggg atagcgctcg tacagcatct ggcgcaccat ctccgccacg
    15541 tactcggcgt gcacggcgta ctcgttgctg tcgccgcctt tcttcacgct caagggggcc
    15601 ttttgcgcct ctgcgtactg cgcgtcgtcg atgtggccga gctcgtgcat gcgccgcagg
    15661 acgtacagct ggcgctgctt ggcgcgcttc ggattgacca ccgggttgaa cagcgacggc
    15721 gccttgggca ggccggcgag catggccgtc tccgcgacgt tcagctgcgc gagcgtcttg
    15781 ccgaaataga cctgcgcggc ggcgccgaag ccgtacgcgc gctgccccag gtagatctgg
    15841 ttgatgtaca gctcgaggat ctggtccttg gtcaggctgc tctcgatctt gaacgcgagc
    15901 agcgcctcgt acagcttgcg cgtcagcgtc ttctcgcgcg agaggaagaa attgcgcgcc
    15961 acctgcatgg tgatggtcga ggcaccctgg gccttgccgc cggcagctag gttcgagtac
    16021 gccgcgcgca gcacgccgag gtagtcgacg cccgtgtgct gatagaagcg ctcgtcctcg
    16081 gcggagagga tcgcctgctg catgatcatc ggcacttcct cgatctttac gaaggagcgg
    16141 cgctcctcgc cgaactcgcc gatcaggacg ccgtcggccg tgtacacccg cagcgggatc
    16201 ttcggctggt attccgtgag cgaggcaatc gaaggcaggt tgggatacgt gagcagcaga
    16261 acgaagccga gcacggccag gccggcgagc gccgtgccgg ccagtgcggc gacaggaaag
    16321 aaaagccagc gcttcatccg gcgccgaccc tggacgaggc cgcgaagccc acttcgagga
    16381 ttagtgaaaa cagtggctta ggtgttgcgt gcggcaagac ggtggttccg acaaaatggc
    16441 ggtcccggga cgagacaaca agtcgattca ggggtcgcga gcacattata aatcgaagcg
    16501 gccgcggcac tgtaagctgt tgcatttcca agttaataat tcactttaca gtgtacatgg
    16561 ttgttgctag catttaacgt aaattatact aatcagtcct aaccggccat aacagaaagg
    16621 gaaatccggt ttgaacctcg acttcctgaa acccaaggcg ccgccgttgt tcgggatgga
    16681 catctcgtcg tcgtccgtca agatggtcga actcgtggaa gcgggcaagg gcagcccgaa
    16741 agtggaccgc tacgtgatcg aggcgctgcc gaaggacgca gtcgccgacg gcaacatcac
    16801 gaacctcgaa gcggtcgtcg acaccgtgca gcgcggctgg aagaagctcg ggactcgcac
    16861 caagaatgtc gcgcttgcgc tcccgaccgc cgccgtgatc acgaagaaga tcattgttcc
    16921 agccggcctg cgcgaagagg agctcgagct ccaggtcgag ggtgaggcca accagtacat
    16981 tccgttcgcg ctcgacgagg tcaacctgga cttccaggtg gtcgggcagg ctccctcggg
    17041 tcccgaggag gtggaggtac tgatcgccgc atcgcgcaag gagaaggtcg acgaccgcat
    17101 cgccgtcgcc gaggcgtccg gcctcaaggc gctgatcatg gacgtcgagg cgtacgcgat
    17161 gcaggcgtcg ttcgagctga tccaacggca gttcccggac gacggcaagg accagaacgt
    17221 cgcgctcgtg gacatcggcg gcaacgtcac caacatcacg gtcctgcgca acgactcggc
    17281 ggtgtacacg cgcgagcagg ccttcggcgg ctcgcagctc acgcaggaca tcatgcggca
    17341 gtacggcatg agccaggagg aagctgagac ggcgaaacgc gccggcggcc tccccgacaa
    17401 ctacgaggcc gaggtgcttc ggccgttcat ggagaacgcc gcgctggaga ttcagcgtgc
    17461 gatgcagttc ttcttcacgt cgacccagta caactcggtg gagcatatcg tgctcaccgg
    17521 cggctcggcg gtgatcccgg gccttgagga tatggtgcag cagcgcacgc aggtgagcac
    17581 catcgtcgcc aatcctttcg cgagcatgca ggtgtcgtcc cgcgttccgc tgaagaagct
    17641 cgttgccgac gcaccgtccc tgatggtggc ctgcggactg gcattgcgga ggttcgacgc
    17701 atgatccgca tcaacctgct gccctggcgg gcggagaggc gcaaggccca acgcaaatac
    17761 ctcgggacgc tggccgccat ggtgggcggg ttgggcctcg cgatcgtcgt gctcgtgcac
    17821 ggcgtgatcg cgggatacat cacgatccag aacgatcgca acgagttcct gaagaaggag
    17881 aacgcgcgcc tcgacaagga aatcgacgaa atcaagaagc tcaagaacga gatcgcggct
    17941 ctgctcgccc gcaagcaggt aatcgagcgg ctgcagaccg accgagcgca ggcggtgtac
    18001 ctgctgcagg agctggtcca gcaggtgccg gacggtgtct acctgaaatc gatcaagcag
    18061 gccggcttgc gcgtggcgct gaacggttat gcgcagtcga acgcgcgcgt ctcgacgctg
    18121 atgcgcaatc tctcggcttc gccctatctc gagaacccgg acctcgttga gatccgcgcc
    18181 gtcacggtcg ccaacaagcg cctttccgag ttccagatga acgtgagcct gcggccaccg
    18241 cagcaggcgc aagatgccgg caaggcgccc gcgcctgcgg cggcggcgaa gaagggctag
    18301 cgcgatgaag cagctgctcg aacagttcaa gaacctgaac atgcgggacc cgggcgcgtg
    18361 gccgccgatc ccgaagatca tgctgctggt cctgatcctc gccggggtgc tgggcggcgg
    18421 ctatatgttc gactggcagg accagctcaa agaactcgac gatggcgccg cgctggaagt
    18481 gaagctcaag gacgactacg tcaacaagga gcggcaggcg atcaacctgg atctctaccg
    18541 ccagcagttg cgcgagatcg acagctcgtt cggcacgttg ctgaagcagc tgcccaaccg
    18601 ttcgcagatg gacgcgcttc tggtggacat caaccaggcc ggcctcggcc gcgggctgca
    18661 atttgagctg ttcaagccgg cgcccgcgga gaccaagcgc gagttctacg ccgagctgcc
    18721 ggtcacgctg aagctgaacg gcacctatca cgatatgggt cacttcgcga gcgatatcgg
    18781 caagctctcg cgcatcgtga cgctcaatga catctcgctg actgcgacgg ccggcggcat
    18841 gctgacgatg gacacggtcg ccaagacgtt ccgatacctg gacgacgacg agatcgcggc
    18901 gcaacggccc gcgaggccgg gtaagaaatg agaaaggccc tcgcgctcct gctcgtcctg
    18961 ctcctcgccg cctgcggcgg tgaggagttc ggcgatctca aggccgagct taaggacaag
    19021 acgaaggatc tccgcgggcg catcgatccg ctgccggtcg tgaagcccta tgagccggtc
    19081 ccgtacaaag tgttcgacca gcccgatccg ttcggcccga gcaagatcga gctggtcacg
    19141 aaggctgcgc ccggagccgg tgggggcctc aagccggatc tgaaccgacc gaaggaacct
    19201 ctggaagcct atccgctcga gtcgctgaag atggtcggcg tttggcagca gcccaaggcc
    19261 acgttcgcgc tggtgaaggc cgataccggc ctgtatcgcg tgaaggtcgg caactacatg
    19321 gggcagaact tcgggctcgt caccgcaatc aacggaagtt ccatccagct tcgcgagctg
    19381 attcaggacg cgacgggcga ctggtccgag cgccagagca cgctgcagtt gcaggagacg
    19441 gggaggtagc gcgtttcggc agtccgcggg ccaagagagc agggggtaga gaatgaacgt
    19501 cctcgcaaga acgacaagaa cgatgcaggc ggcgctgctg gtactgggcc tgctcgcgat
    19561 cgcgccgctc gcccgagcgc aaaacgcgct cgaagggttc gaggtcgcgc accaggcggg
    19621 cgacatcgtg atcaaggtga cgctgaagga ggcgctgacc accgccccgg gcagcttcac
    19681 cgtggccaac ccggcgcgca ttgctttcga cttcccgagt accatcaacg ctctcggccg
    19741 caacaaccag accgtcggtg aaggcgacct gcggtccatg aacgtggtgc aggccggcga
    19801 ccggacccgt ctggtgctga acctgcgcaa catgatgcag tacgacgcca aggtcgacgg
    19861 ccgcaacgtt gtgatcacgc tgaccaaggg aacggccccc gcgaccgccc aggccgacgg
    19921 tgcggcgccg gcggcgcgct tcgccgaggg acgcaccgat gtcacgcacc agatcaagga
    19981 catcaatttc cggcgcgggc gtaatggaga aggccggatc gtcgtcgatc tgtccgacac
    20041 gtcgaccggc gtcgacatcc gccagcaggg ccagaccctc atcgtcgact tcctgagggc
    20101 caacctgccg gatgccctgc gcaagcggct cgacgtggtc gatttcgcaa cgccggtgca
    20161 gacggtaagc gccttccctc agggcgacaa cgttcggctg ctgatcgagc cgaagggcct
    20221 gtgggagcac aacgcgtatc agactgacac gcagttcgtg ctggaagtga agccggtcca
    20281 gtacgacccg aacaagctgg tccagggcac gcgcggcggc tatacgggtg agaagctctc
    20341 gctgaacttc cagaacgtcg aagttcggag cgttctcaac gtcatcgccg actttaccga
    20401 cctcaacatc atcaccagcg actcggtgag cggaaacctg acgctgcggc tgaaggacgt
    20461 gccctgggac caggcgctcg acatcatcct gcagacgcgc ggcctcgata tgcgcaagaa
    20521 cggcaacgtg atctggatcg cccctcgcga cgagcttgcc accaaggaga agctgagcct
    20581 cgaatcgcag gcgcagatca cggagctcga gccggtccgc accgagaact tccagctcaa
    20641 ttacgccaag gccctggaca tggtgaagat cctgagcgat ccgaaccaga agatcctttc
    20701 gaagcggggc aacgcggtcg tggacgcgcg gaccaatcag ctgttcgtgc aggacgtgcc
    20761 cagccgcctc gaggaggtgc gccggctgct ggcgaagatg gacgtgcccg tgcgccaggt
    20821 gctgatcgag gcgcggattg tcgaggcgaa cgatacgttc agcaagaacc tgggcgttcg
    20881 cctgggcgtt cacgatctcg gcggcgatca tcgactggcc gggacgaaca cccacgtcga
    20941 cgtcggtggc ggcctgggag acaccggcgg ccacgcggcc ggtggagcac agggcggtac
    21001 accggtcttc atcccggaca gcctgagcgt gaacgtgccg tcgccaggct tgagcggctt
    21061 caacccgggg cagttgtccg tgatcctgtt caacagcacc ttctcgcagt tcctgaacct
    21121 ggagctgagc gccctcgaat cggaaggcaa gggcaagatc atctccagcc cgcgcgttct
    21181 aacggccgac aacatcgagg ccctcatcga gcaaggcacc gagcttccat accagcaggc
    21241 cacatcgagc ggcgcgaccg cgatttcgtt ccggaaggcc aatcttagac tgctggtcaa
    21301 gccgcagatc acgcccgacg gcaacgtgat catgagcctg gacgtcaata aggacgccgt
    21361 cggccagatc accactgcgg gtttcgccat cgacaccaag cacgtcaaga cggaagtgct
    21421 ggtcgagaac ggcggcaccg tcgtgatcgg cggcatctat acgcaggacc tgcgcaacca
    21481 ggttgtcaag attccgttct tcggcgacct tcccgtggtc ggtttcctgt tccgcaatac
    21541 gcagcgccag gacaacaaga ccgagctctt ggtgttcatc acgccgcgca tcgtcgaaga
    21601 ccgcatgctc ggctcgcgct gagctgctct ccgcttgccg ccgagagccg gcctgcgccg
    21661 gctttcggcg ttttagggcg cctggccggc tcgggctaca atgacgaggt gaagacctcg
    21721 cgaaatcttt tcctcgtcgg tctcatgggt gccggcaaga ccaccgtcgg gcgcgcgctg
    21781 gcgaaacgcc tgggcaaggc gttccacgac tccgaccgcg agatcgaggc gcgcaccggc
    21841 gtgcgcgtcg cgcacatctt cgagatcgaa ggcgaggacg ggttccgcag gcgcgaggcc
    21901 gaggcgatcg agcgcctcac cgcgctcgac gacatcgtcc tcgccaccgg cggcggcgcc
    21961 gtgctcgacc cggccaatcg cgaccggctc aagagccggg gcttcgtcat ctatctgcac
    22021 gcacatccgc gcgagctctg gcagcgcacg cggcacgacc gcacccggcc gctgctgcag
    22081 agcgccgatc cgcgcgggcg cctcgaggag ctgtatgcgc tacgcgatcc gctctaccgc
    22141 gaagtcgccg atgccgtggt cgagaccggc cggcaaggcg taactctcct ggtcgcccag
    22201 gcgctcgcca agatcgagga cgcatgcaga ctgtccgcgt agcgctcggc gagcgctcgt
    22261 atccggtgca tatcggctcc ggcctgctcg gacgcgccga gctggtgctc ccgcagctgc
    22321 cgcgcaaaca gatcgcggtc gtgaccgaca cgaacgtcgc gccgctctac ctcgagcgct
    22381 tcgccggccc gctgcgggaa gccggcgtcg acgtcgtcac cgtcgtgctc cccgatggcg
    22441 aagcgcacaa gcgctgggac agcgtcgagc gcgtcctcga tacgctgctc gcgcgccgct
    22501 gcggccgcga caccacgctg gccgcgctcg gcggcggcgt ggtcggcgac ctgaccgggt
    22561 tcgcggcgtc cgtctaccag cgcggggtgc cgtacatcca ggtgcccacc acgctgctcg
    22621 cccaggtcga ttcgtcggtc ggcgggaaga ccgcgatcaa ccatgcgcgc ggcaagaaca
    22681 tggtcggcgc gttccaccag ccggcgctcg tgctcgcgga catggacacg ctggtgtcgc
    22741 tgcccgcacg cgagctgcgc gcagggctgg ccgaggtgat caagcacggg ttcatccggg
    22801 acgccgattt cgtcggctgg ctggaagcct acgtcgacga cctgctcgcc cgggatcgcg
    22861 atgcgctcgc gcacgcggtc gcgcgctcgg tggagatcaa gtcggcgatc gtcgcgcgcg
    22921 acgagcgcga gcagggagag cgcgcgctcc tcaacttcgg ccataccttc gggcacgcgc
    22981 tcgagaccgg gctcggctac ggcgagtggc tgcacggcga ggcggtcgcg gccgggatgg
    23041 cgatggcggc cgacctctcg cgccgcctgg gaatcctgag cagcgccgac tgcgagcgtg
    23101 tgcgggcgct cctcgctcgc gccgggctgc cggtcgaggc gccggcactg cccgtgcagc
    23161 ggctgctcga gctgatgagc gtcgacaaga aggtgaaaga cgggcgcctc aggttcatcc
    23221 tgctcgagcg catcggcgcc gcggtgctgc gcgcagacgt gcctcgggcg gtgctcgagg
    23281 agacgctcgc tgcggcgacc ccgcaacgct ccctaagcac ttgaggctgc agggaatcga
    23341 cggtatagtt caaggttcgc cctgaattcg agcgaattcg ggttttttgg cgcgcccgga
    23401 cgaggtccac ccgtgaatca tcccgctctc cccgccgcgc aggggctgta cgaccctgcc
    23461 cacgaacatg acgcctgcgg cgtcggattc gtcgcgaaca tcaaggggaa gaagagccac
    23521 tcgatcatcg agcaggggct cacggtcctg cgcaacctga cgcaccgagg cgcggtcggc
    23581 tgggatccga agctctcgga cggcgccggc atcctcatcc agatcccgga caagtttttc
    23641 cgcgaggagc tcgggaaggc cggcatcaag ctcccgccgg tcggccagta cggcgtcggc
    23701 atggtgttcc tgccgcgcga gcccgcgtct cgcctggcct gcgaatacga gatcgagcgc
    23761 gcgatcaagg acgagggcca ggtcctgctc ggctggcgcg acgtgccggt cgacaattcc
    23821 gacctcgccg agccggcgaa gcgcctcgag ccggtcatcc gccaggtctt cgtcggacgc
    23881 ggcccgggcg tgaccgtgac cgatgcgctc gagcgcaagc tctacatcat ccgcaagtcc
    23941 tcggggcacg cgatccaggc gctcaagctc gcgcacggca aggagttcta cgtgccgtcg
    24001 atgtccgcgc ggacgatcgt ctacaagggc atgctgctcg cgcaccaggt cggggagtac
    24061 tacctcgacc tcaaggacgc gcgcgtcgag tcggcgctcg cgctcatcca ccagcgcttc
    24121 tcgaccaaca cgttcccgtc gtgggacctc gcgcacccgt tccgcatgat cgcgcacaac
    24181 ggcgagatca acaccctgcg cggcaacgtc aactggctgc gcgcgcgcca aggcgcgatc
    24241 tccagcccga tcctcggcgc cgacctgaac aagatctggc cgctgatcta cgacgggcag
    24301 tccgactcgg cttcgttcga caacgcgctc gagctgctcg tgatgagcgg ctactcggtc
    24361 gcgcacgcga tgatgatgat gatcccggag gcgtgggaga gccacaccct catggacccc
    24421 gcgcggcgcg ccttctacga ataccacgcg gcgatgatgg agccctggga cgggcccgct
    24481 tccatcgcgt tcaccgacgg gcgcctgatc ggcgcgacgc tcgaccgcaa cggcctgcgg
    24541 ccctcgcgct atatcgtcac cgacgacaac ctcgtgatca tgggctcgga gtgcggctgc
    24601 ctgccggtgc ccgaggagcg catcaccaag aagtggcgcc tgcagcccgg caagatgttc
    24661 ctggtcgaca tcgagaaggg ccgcatcatc gacgacaagg agctgaagga gacgctcgcc
    24721 tcggccaagc cctacgccga gtggatcgac cgcatccgcg tgaagctcga cgaggtcgag
    24781 agcgacaagc agcgccccga gcgctcgcgc gtgccgctga tcgaccgcca gcaggccttc
    24841 ggctattcgc aggaggacgt caagattctg atgacgccga tggcggcgaa cggcgaggag
    24901 ccggtgggct cgatgggcaa cgactcgccg ctcgcggtac tgtcgtcgaa gaacaagacg
    24961 ctgtaccact atttcaagca gctgttcgcg caggtcacca acccgccgat cgacccgatc
    25021 cgcgaggagc tggtcacgtc gctcgtctcg ttcatcgggc cgaagccgaa cctgctcggg
    25081 gtcgacgaga cgaacccgcc gctgcggctc gaggtgagcc agcccgtgct cgacttcttc
    25141 gagatggaga agatccgaca catcgagcgc tatacccagg gcaagttcca ggtgttcgag
    25201 ctcgacatca cctatccgct cgcctggggc aaggaggcga tcgaggcgcg gctcgcgagc
    25261 ctcgccgcgc aggccgagga cgcggtgcgc tcagggcatt cgatcatcat catctccgac
    25321 cggaagatcg accgcgaccg cgtcgcgatt ccggccctgc tcgcgctttc ggccgtgcac
    25381 cagcacctgg tcggcaaggg cctgcggacg tcgaccggcc tggtggtcga gaccggctcg
    25441 gcgcgcgagg tgcaccactt cgcgctcctc ggcggctacg gcgcggaggc cgtgcatccc
    25501 tacctcgcgc tcgagacgct gctcgacctc gcggacaagg gcgcgctggg cgccggcgtc
    25561 gacggccgaa aggcggtcaa gaacttcgtc aaggccatcg gcaaggggct gaagaaggtc
    25621 gcgtcgaaga tggggatctc gacctacatg tcgtacacgg gggcccagat cttcgaggcg
    25681 gtgggactgt cgcgcaagct cgtcgacaag tacttcaccg gcacgacgtc caacgtcgag
    25741 ggcatcggcg ttttcgaggt cgcggaggaa gcgatccgcg tgcaccgcgc cgccttcagc
    25801 gagaccgatc cggtgctcgc gaccgcgctc gacgcgggcg gcgagtacca ctaccgggtc
    25861 cgcggcgagg agcacatgtg gactcccgac gcgatcgcca agctccagca ctcggtgcgc
    25921 aagggcgagt tcgccacgtt caaggagtac tcgcgcatca tcgacgacca gtcgaagcgc
    25981 cacatgacgt tccgcggcct gttcgagttc cgcttcgacc gcgccacgcc ggtgccgatc
    26041 gaggaggtcg agcccgcgtc cgagatcgtc aagcgcttct cgaccggcgc gatgtcgctc
    26101 gggtcgatct cgaccgaggc gcacaccacc ctcgcgatcg cgatgaaccg catcggcggc
    26161 aagtcgaaca cgggcgaagg cggcgaggat cgtaaccgct atgcgcgggc gaaggcgggc
    26221 gagacgctcg cctcgcgcct cggcaaggat cgcgtgatgg tcgacattcc gctgaaggaa
    26281 ggcgacctgc tcaagtccaa gatcaagcag gtggcctcgg gccgcttcgg cgtcaccgcc
    26341 gagtacctcg cgagcgccga gcagctgcag atcaagatgg cgcagggcgc gaagccaggc
    26401 gaaggcgggc agctcccggg ccgcaaggtg tccgagtaca tcgcgcagat ccgctactcg
    26461 acccccggcg tcgagctgat cagcccgccg ccgcaccacg acatctactc gatcgaggac
    26521 ctggcgcagc tgatccacga cctgaagaac gcgaaccccg aggcgtcggt gagcgtgaag
    26581 ctggtgtcgg aagtgggcgt gggtaccgtg gccgcgggcg tctccaaggc caaggccgac
    26641 cacgtcacca tctccggcca cgacggcggc acgggcgcct cgccctggtc gtcgatcaag
    26701 cacgcgggca cgccgtggga gctgggcctc gccgagacgc agcagacgct ggtgctgaac
    26761 cgcctgcgcg gccgcatcgc ggtgcaggtc gacggccaga tgaagaccgg ccgcgacatc
    26821 gtgatcggcg cgatgctcgg cgccgacgag ttcggcttcg ccaccgcgcc gctcgtcgcc
    26881 gagggctgca tcatgatgcg caagtgccac ctgaacacct gcccggtcgg cgtcgcgacg
    26941 caggacccgg tgctccggaa gaagttcgcg ggcaggcccg agcacgtcat caactatttc
    27001 ttcttcgtcg ccgaggaggc gcgcgagctg atggcgaagc tcggcgtgcg cacgttcaac
    27061 gagctgatcg gccgcgccga cctcctcgac acgaagaagg gcgtcgacca ctggaaggcg
    27121 aagggcctcg acttcagccg gatcttccac ctgccgcaga tgcccgggga cgtggcccgc
    27181 tggaacagcg agcgccagaa ccacggcctc gagcgcgcgc tcgaccatcg cctgatcgag
    27241 atcgcgaagc ccgcgctcga gcgcggcgag aaggtcgcga tcgacatgcc gatccgcaac
    27301 ctcaaccgca ccgtcggcac catgctctcg cacgagatcg ccaggcgcta cgggcacgac
    27361 gggctgccgg acgacaccat ccacatccgc ttcgccggct cggccgggca gagcttcggc
    27421 gcgttcctgg cgaaaggcgt gacgctcgac ctgatcggcg acaccaacga ctactgcggc
    27481 aagggcctct ccggcgggcg cattaccgtg cagccctcgc cgaagttccg cggcgagccg
    27541 acgcagaacg tgatcaccgg caacgtcgtc ttgtacggcg cgatcgcggg cgaagcttat
    27601 ttccgcggcg tggcgggcga gcgcttcgcg gtgcgcaact cgggcgctca agccgtcgtc
    27661 g
//
LOCUS       DATDMW010000050         8631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1132584, whole genome shotgun sequence.
ACCESSION   DATDMW010000050 DATDMW010000000
VERSION     DATDMW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132584"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VI319_02185"
     CDS             <1..830
                     /locus_tag="VI319_02185"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6862682.1"
                     /translation="AYNIADSVANYHRALTVEANAIQDKYQFVFDARPGAGGAIASNY
                     VLNTPNTILAHSTAFFIRPNVYPKESYDLGRFQELFNHCTASMAVTSVKYTDWKEVPG
                     RPVTVGVSGLGVTTHLAALQLQKKFPNLNIIPFKSTTDSMFSMVGGNTDFHIGFISEA
                     EQWTRKKDKGAVNILGITGPKAVSGYRTLVSEGFPQAFAQMDVGHHLVVPIGRFPEEK
                     AREIYDIFSKAAKRKAVRDAYAVDFCVPAETAYEDLQKWYGFHTEYWKKLSAGINLD"
     gene            complement(838..913)
                     /locus_tag="VI319_02190"
     tRNA            complement(838..913)
                     /locus_tag="VI319_02190"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(878..880),aa:Thr,seq:tgt)
     gene            complement(957..1577)
                     /locus_tag="VI319_02195"
     CDS             complement(957..1577)
                     /locus_tag="VI319_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6862683.1"
                     /translation="MSKPLIYVVDDDDTFRKSLQWLLESVGLAVRTFTNAAEFLESYV
                     PGSPGCLVLDIRMPGISGLQLQDQLAGRGIGLPVIFLTGHGDVPMAVAAVKKGALDFI
                     QKPYNDQQLLDLVNSALKRDAELREQEARSHQIASLVGSMTPREREVMDAVVSGKSNK
                     IIAEELGVTIKTVEAHRARVMEKMGAESLAHLVHLVMTARGARVTT"
     gene            complement(1580..3034)
                     /locus_tag="VI319_02200"
     CDS             complement(1580..3034)
                     /locus_tag="VI319_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6862684.1"
                     /translation="MLVDAFRRYISSRSPARVLPYAVALVFVVAAINYATGPDLSLAI
                     LYLLPIAAGTWAGGRVTGFAFSVLAAVLWMTTDTLNGHRYTNPLYFHWEWAVRLATHL
                     VFVVVLDHLKSALARADEALQKSEGRYQALVAALPYGILEVDLAGRVLVVNPALGEML
                     RSPEARLRERPVEELFENPEKAAAEPRALSDRSGAAVCYLGRLKRADGGAIDARVDWV
                     CKRDARGAPAGHIAVVTDITEQKQAEETSRKQEERLQLVSRLITVGEMASTLAHELNQ
                     PLASIVNFNMGCVRRIRSGSWNAPDLLGALEQASAQAERAGQIIQRIRDMVAKREPTR
                     TALDVNAVIRELARLVELDAGKKGVRLVLDLAPGLPPVRADKVMIEQVVLNLVKNAVE
                     AMEQTDPEQRQVTMRSAVNGGRSVEIALTDRGVGVPQELCDGQFTPFFTTKRDGMGLG
                     LSICRSIIEFHDGRLTANRNPQGGSTFRFTLPMA"
     gene            complement(3106..3522)
                     /locus_tag="VI319_02205"
     CDS             complement(3106..3522)
                     /locus_tag="VI319_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEY6862685.1"
                     /translation="MSEPTSTKPYLLRAIYEWCVDNGYTPYVSVVVDARTRVPMEYVR
                     DGEIVLNIGPAATDRLLIGNELIECSARFAGTVRELSIPVAAVAAIYARENGHGMSFE
                     AGARTAAEAGDEAGTAGPGDVPEPPKPGKPNLRRIK"
     gene            complement(3591..4190)
                     /locus_tag="VI319_02210"
     CDS             complement(3591..4190)
                     /locus_tag="VI319_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6862686.1"
                     /translation="MMNLYSGTTCPFSQRCRIVLYEKGMDFQIIDVDMYNKPEDIAVM
                     NPYNRLPVLVERDLILYESNIINEYIDERFPHPQLMPADPVMRARARLFLFNFEVELF
                     SQIETLEHGNQKQQDRARQHVSDRLTELAPVFTKQKYMLGDEFSMLDVAISPLLWRLD
                     YYGISRPKAAAPLMKYAERLFSRPAFIEALTPSEKVMRK"
     gene            complement(4218..4988)
                     /locus_tag="VI319_02215"
     CDS             complement(4218..4988)
                     /locus_tag="VI319_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY6862687.1"
                     /translation="MKNILALAALACACVVHPLALAQEEEPRLEAAPIDPRDLTSLQA
                     GARTFVNYCLNCHSASLMRYSKLEDIGLTEQEIKDNLLFTGEKIGDVMDVAMPRKEAK
                     EWFGAAPPDLSVIGRSRSADWLYTYLRGFYRDPSRPTGWNNVVYPNVAMPHVLWQLQG
                     QQALEPAAKGAEEKGVAKKPESEAEPAPAKLKLAVAGRLTPVEYDVVVRDLVNYLVWM
                     SEPEARVRKRVGVVAVFFLGILVVLTYALYKEFWKDVH"
     gene            complement(4985..6391)
                     /locus_tag="VI319_02220"
     CDS             complement(4985..6391)
                     /locus_tag="VI319_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEY6862688.1"
                     /translation="MAVIEVDKKPVTGAGPFNGVLTWIDQRFPLMSNWREHLSEYWAP
                     KNFNFWYYFGSLALLVLVIQIVTGIFLTMNYKPDAKEAFASVEYIMREVNWGWWIRYM
                     HSTGASFFFICVYLHMFRGLLYGSYRRPRELVWMVGMLIFLSLMAEAFFGYLLPWGQM
                     SYWGAQVIVNLFDAIPLIGPKLAIWIRGDYVVSDATLNRFFAFHVIAVPLVLLGLVVA
                     HILALHEVGSNNPDGVDIKKTKDAATGHPIDGVPFHPYYTVKDSFGASVFLVIFFIVV
                     FFLPEMGGYFLESNNFIPADPLKTPPHIAPVWYFTPFYSILRATTDNFVYFGLIPGVI
                     LLALAIIVTVPRTAVRVAVAVLAVLLLAGFLGFEAKVWGVVLMGVSVLIYFFLPWLDR
                     SPVKSIRYKGAYYKIALALFVVAFFVLGYLGTQPVSTGRTVLAQVCTAVYFLFFAVMP
                     WYSRFDKTKPEPERVTWK"
     gene            complement(6399..7004)
                     /gene="petA"
                     /locus_tag="VI319_02225"
     CDS             complement(6399..7004)
                     /gene="petA"
                     /locus_tag="VI319_02225"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY6862689.1"
                     /translation="MSDSPETTNRGRRNIIALTAAAGGAAAVTGAIPLVASMLPSEKA
                     KALGAPVEFDLSQVPPGAKATVEWRGQPVWVLHRTKEMLDDIKKDDEKVADPKSERNK
                     DLTPDYARNEFRSIKPEYLIVVGICTHLGCSPQDRLQSGSEPFDPSWAGGFYCPCHGS
                     LFDLAGRVYKNKPAPFNLRVPPYTYLSDNKILIGEDKVKKG"
     gene            7208..8350
                     /locus_tag="VI319_02230"
     CDS             7208..8350
                     /locus_tag="VI319_02230"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY6862690.1"
                     /translation="MRRLWLIFAQATTVALGALFVVVTLKPEWLGRPGGTEVVELTQA
                     VPAGAGSPRSSSYSDAVRRATPAVVNIFTSKEVKTPPHPFLNDPLFRHFFGDRADPES
                     QRAASLGSGVIVSTRGYILTNHHVVEAADEIEVALADGKKLKARLVGSDPETDLAVLQ
                     VSNGTLPAITFGDTESLRVGDVVLAIGNPFGVGQTVTMGIISALGRNQLGINTFENFI
                     QTDAAINPGNSGGALIDAAGNLIGINTAIYSRSGGSLGIGFAIPASSAKQVMEQIVAS
                     GSVTRGWIGVEAQELTPDLPGGADANGVLIAGVLRGGPADKAGFRPGDVLIAIDGKQV
                     KDPSAMLNAVAALVPGRTAAVRLRRDEKNIDVNVEVGRRPPPTLRR"
     gene            complement(8355..>8631)
                     /locus_tag="VI319_02235"
     CDS             complement(8355..>8631)
                     /locus_tag="VI319_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY6862691.1"
                     /translation="AFGITFEVPVVVVVLVHLGVVELAKLRSIRGYVIVGAFVVGAIF
                     TPPDVLSQVMLAVPLWLLFEVGLVLARFLGRPAAADAPRLEEPEVEK"
BASE COUNT         1502 a         2840 c         2910 g         1379 t
ORIGIN      
        1 acgcgtacaa catcgcggac agcgtcgcga actatcaccg cgcgctgacg gtcgaggcga
       61 acgcgatcca ggacaagtac cagttcgtct tcgacgcgcg ccccggagcg ggcggcgcca
      121 tcgcgagcaa ctacgtgctg aacacgccga acacgatcct cgcgcacagc acggcgttct
      181 tcatccgccc caatgtctat ccgaaggaga gctacgatct cggccggttc caggagctgt
      241 tcaaccactg cacggcctcg atggccgtca ccagcgtgaa atacaccgac tggaaggaag
      301 tcccgggcag gccggtcacg gtcggcgtga gcgggctcgg cgtcacgacg catctcgcgg
      361 cgctgcagct acagaagaag ttcccgaacc tgaacatcat ccccttcaag tccaccaccg
      421 actccatgtt ctccatggtc ggcggcaaca ccgacttcca catcggcttc atcagcgaag
      481 ccgagcaatg gacccggaag aaggacaaag gcgcggtcaa catcctgggc atcaccggcc
      541 ccaaggcggt gagcggctat cgcacgctcg tgagcgaggg cttcccccag gcgttcgcgc
      601 agatggacgt cgggcaccac ctggtcgtgc ccatcggccg gttcccggag gagaaggcgc
      661 gcgagatcta cgacatcttc agcaaggccg cgaagcgcaa ggccgtgcgc gacgcctacg
      721 cggtcgattt ctgcgtcccg gcggagaccg cctacgagga cctgcagaag tggtacggtt
      781 tccacaccga gtactggaag aagctgagtg cgggcatcaa cctcgactag cgatcgatgg
      841 tgccgggtag aggagtcgaa cccccgacct tctgattaca aatcagctgc tctaccagct
      901 gagctaaccc ggcacgccgg cgcgattata ccgggtgtcg ccgggggccc cgcccctcag
      961 gtcgtgacgc gggccccgcg cgcggtcatc accaggtgca cgaggtgcgc gagcgactcc
     1021 gcgcccatct tctccatgac gcgggcacgg tgcgcctcga cggtcttgat ggtcacgccg
     1081 agctcctcgg cgatgatctt gttcgatttg cccgagacga ccgcgtccat cacctcgcgc
     1141 tcgcgcggcg tcatcgagcc gaccaggctc gcgatctgat ggcttcgcgc ttcctgctcg
     1201 cgcagctcgg cgtcgcgctt gagcgccgag ttgacgaggt cgagcagctg ctggtcgttg
     1261 tacggcttct ggatgaagtc gagcgcgccc ttcttcaccg cggccaccgc catcggcacg
     1321 tcgccgtggc ccgtgaggaa gatgacgggc aggccgatgc cgcgcccggc gagctggtcc
     1381 tgcagctgca ggccgctgat gcccggcatg cggatgtcga gcaccaggca cccgggcgag
     1441 cccggcacgt agctctcgag gaactcggcc gcgttcgtga acgtgcggac cgcgaggccc
     1501 accgactcga gcagccattg cagggacttg cggaaggtat cgtcgtcgtc gacgacgtag
     1561 atcagcggtt tgctcatgtt catgccatgg gtagtgtgaa acgaaaagtg ctgccgcctt
     1621 gcggattccg gttcgcggtg aggcggccgt cgtggaactc gatgatcgag cggcagatcg
     1681 acaggcccag ccccatgccg tcgcgcttgg tggtgaagaa cggcgtgaac tggccgtcgc
     1741 agagctcctg cgggacgccg acgccgcgat cggtgagcgc gatctcgacc gagcggccgc
     1801 cgttgaccgc ggacctcatg gtgacctggc gctgctcggg atcggtctgc tccatggcct
     1861 cgacggcgtt cttgaccaga ttcaggacca cctgctcgat catcaccttg tcggcacgca
     1921 ccggaggcag gccgggagcg aggtcgagga cgaggcgcac gcccttcttg ccagcgtcca
     1981 gctcgaccag gcgcgcgagc tcgcggatga cggcgttcac gtcgagcgcg gtgcgggtcg
     2041 gctcgcgctt ggcgaccatg tcgcggatgc gctggatgat ctggcccgcg cgctcggcct
     2101 gcgcgctcgc ctgctcgagc gcgccgagca ggtccggcgc gttccaggat cccgaccgta
     2161 tgcgacgcac gcagcccatg ttgaagttca cgatcgaagc gagcggctgg ttcagctcgt
     2221 gcgcgagggt cgacgccatc tcgcccaccg tgatcagacg cgagacgagc tgcaggcgtt
     2281 cctcctgctt gcggctcgtc tcctcggcct gcttctgctc ggtgatgtcg gtgaccaccg
     2341 cgatgtgccc ggcgggcgca ccgcgcgcgt cgcgcttgca gacccagtcg acgcgtgcgt
     2401 cgatcgcacc gccgtcggca cgcttgaggc gacccaggta gcagaccgcc gcgccgcttc
     2461 ggtccgagag cgcgcgcggc tcggccgcgg ccttctcggg attctcgaag agctcctcga
     2521 ccggccgctc gcgcaggcgc gcctccggcg agcgcagcat ctcgccgagc gccgggttca
     2581 cgaccaggac gcgcccggcg agatcgacct cgaggatgcc gtagggcagc gccgcgacca
     2641 gagcctggta gcgcccctcg cttttctgca gcgcctcgtc ggcccgggcg agcgcggatt
     2701 tcaggtgatc gagcaccacc acgaacacga gatgagtcgc gagccggacc gcccactccc
     2761 aatggaagta gagcgggttg gtgtagcggt gtccgttcag cgtgtcggtc gtcatccaca
     2821 ggacggcggc gagaaccgag aaggcgaagc cggtcacgcg gccgcccgcc caggtgcctg
     2881 ccgcaatcgg gagcaggtac aggatggcga gcgacaggtc cggtccggtg gcgtagttga
     2941 tcgccgcgac cacgaagacg agcgcgaccg cgtacggcag cacccgggcg gggctgcgcg
     3001 aagagatgta gcgacggaac gcgtcgacga gcatttgctg cgggaaggta cgccgggacc
     3061 cggccaagat accaccgcgg cttgccgctt gtgaacagcg gccggctatt taatgcgcct
     3121 gaggttgggt ttacccggct tcgggggctc cggtacatcc ccgggcccgg ccgtgccggc
     3181 ctcgtccccg gcctccgcgg cggtccgcgc gcccgcctcg aacgacatgc cgtggccgtt
     3241 ctcgcgcgcg tagatcgcgg cgactgcggc gaccgggatg gacaattcgc gcaccgtgcc
     3301 cgcgaagcgg gcgctgcact cgatcagctc gttgccgatc agcaggcggt ccgtcgcggc
     3361 cggcccgatg ttgagcacga tctcgccgtc gcgcacgtac tccatcggca cccgcgtacg
     3421 ggcgtccacg accacggaaa cgtagggcgt gtagccgttg tcgacgcacc attcgtagat
     3481 ggcacgcagg aggtagggct tggtcgacgt cggttcgctc atgaataagc agatccttcg
     3541 tcgcgtggct cctcaggagg atgcgcaatc gcgacgccgc ggccgcggcg tcacttgcgc
     3601 atcaccttct ccgacggcgt cagggcctcg atgaacgccg ggcgcgagaa cagccgctcg
     3661 gcgtacttca tgagcggcgc cgccgccttg ggccgcgaga tgccgtagta gtcgaggcgc
     3721 cacaggagcg gcgagatcgc gacgtcgagc atcgagaatt cgtcgccgag catgtacttc
     3781 tgcttggtga acaccggcgc gagctcggtc aggcggtcgg agacgtgctg gcgcgcgcgg
     3841 tcctgctgct tctggttgcc gtgctcaagg gtctcgatct gcgagaacag ctccacctcg
     3901 aaattgaaca ggaacaggcg tgcgcgcgca cgcatcaccg ggtcggccgg catgagctgc
     3961 gggtgcggga agcgctcgtc gatgtactcg ttgatgatat tggactcgta caggatcagg
     4021 tcgcgctcga ccagcaccgg gaggcggttg tacggattca tcaccgcgat gtcctcgggc
     4081 ttgttgtaca tgtcgacgtc gatgatctgg aagtccatgc ccttctcgta gagcacgatg
     4141 cggcagcgtt gcgagaaggg gcaggtcgtc ccggagtata ggttcatcat ggcagcggtc
     4201 ccgtcgccgt cggcgactca gtgcacgtct ttccagaatt ccttgtagag cgcgtaggtc
     4261 agcaccacca ggatgccgag gaagaacacc gccaccacgc cgacgcgctt ccgaacgcgc
     4321 gcctccggct cggacatcca taccaggtag ttgacgagat cgcgaacgac gacgtcgtac
     4381 tcgaccggcg tgaggcgccc ggccacggcg agcttcagct tcgcgggcgc gggctcggcc
     4441 tcgctctcgg gcttcttcgc cacgcccttc tcctcggcgc ccttggccgc cggctcgagc
     4501 gcctgctggc cctggagctg ccacagcacg tgcggcatgg cgacgttcgg atacacgacg
     4561 ttgttccagc cggtcggccg gctcggatcg cggtagaagc cgcgcaggta cgtgtacagc
     4621 cagtcggcac tgcgcgagcg cccgatcacc gacaggtcgg gcggcgccgc gccgaaccac
     4681 tccttcgcct ccttgcgcgg catcgcgacg tccatgacat cgccgatctt ctcgccggtg
     4741 aagagcaggt tgtccttgat ctcctgctcg gtgaggccga tgtcctcgag cttcgaatag
     4801 cgcatcagcg acgccgagtg gcagttgagg cagtaattga cgaaggtgcg cgcaccggcc
     4861 tgcaggctgg tcaggtcgcg cggatcgatg ggcgcggcct cgaggcgcgg ctcctcctcc
     4921 tgcgcgagcg cgagcgggtg gacgacacag gcgcaggcga gcgccgcgag ggcaaggatg
     4981 ttcttcattt ccacgtcacc ctctcaggct cgggtttggt cttgtcgaat cgcgaatacc
     5041 acggcatcac cgcgaagaac aggaagtaca ccgccgtgca gacctgcgcg agcacggtgc
     5101 gcccggtcga aaccggctgc gtgccgaggt agccgagcac gaagaacgcg accacgaaca
     5161 gcgcgagcgc gatcttgtag tacgcgccct tgtagcggat cgacttcacc gggctgcggt
     5221 cgagccaggg caggaagaaa tagatgagca ccgagacgcc catcagcacg acgccccaga
     5281 ccttcgcctc gaagccgagg aagcccgcga gcagcagcac cgcgagcacc gccaccgcga
     5341 cccgcaccgc ggtgcgcggc acggtgacga tgatcgcgag ggcgagcagg atcacccccg
     5401 ggatgagccc gaagtacacg aagttgtcgg tcgtcgcccg caggatcgaa tagaacggcg
     5461 tgaagtacca caccggggcg atgtgcggcg gcgtcttcag cgggtcggcc gggatgaagt
     5521 tgttggactc gaggaagtag ccgcccatct cgggcaggaa gaacacgacg atgaagaaga
     5581 tcacgaggaa caccgacgcg ccgaacgagt ccttcaccgt gtagtacgga tggaacggga
     5641 cgccgtcgat cgggtgcccg gtggcggcat ctttcgtctt cttgatgtcg acgccgtcgg
     5701 ggttgttcga gccgacctcg tgcagcgcca ggatgtgcgc gaccacgagc ccgagcagca
     5761 cgagcggcac cgcgatcacg tggaacgcga agaagcggtt gagcgtcgcg tccgagacga
     5821 cgtagtcgcc gcggatccag atcgcgagct tcggcccgat cagcgggatc gcgtcgaaca
     5881 ggttcacgat cacctgcgcg ccccagtacg acatctgacc ccagggcagg aggtagccga
     5941 agaaagcctc cgccatgagc gagaggaaga tcagcatgcc gaccatccac accagctcgc
     6001 gcggccggcg gtaggagccg tagagcagcc cgcggaacat gtgcaggtac acgcagatga
     6061 agaagaacga ggcgcccgtg gagtgcatgt agcggatcca ccagccccag ttcacctcgc
     6121 gcatgatgta ctcgaccgag gcgaacgcct ccttcgcgtc gggcttgtag ttcatggtga
     6181 ggaagatgcc cgtgacgatc tggatcacga gcacgaggag cgcgagcgag ccgaagtagt
     6241 accagaagtt gaagttcttc ggcgcccagt actcggacag gtgctcgcgc cagttcgaca
     6301 tcagcgggaa gcgctggtcg atccaggtga gcacgccgtt gaacgggccg gcgcccgtga
     6361 cgggcttctt gtcgacttcg atgacggcca tggcgcccct atcccttctt gaccttgtcc
     6421 tcgccgatga ggatcttgtt gtcggagagg taggtgtacg gcggtaccct caggttgaac
     6481 ggcgcgggct tgttcttgta gacgcgccca gcgagatcga acagcgaccc gtggcacggg
     6541 cagtagaagc cgcccgccca gctcggatcg aacggctcgg acccggactg cagccggtcc
     6601 tgcggcgagc accccaggtg cgtgcagatg ccgacgacga tcaggtactc gggcttgatg
     6661 gagcggaact cgttgcgcgc gtagtcgggc gtcaggtcct tgttgcgctc gctcttcgga
     6721 tcggcgacct tctcgtcgtc cttcttgatg tcatcgagca tctccttcgt ccgatgcagg
     6781 acccacaccg gctgcccgcg ccattcgacc gtcgccttgg cgcccggggg tacttggctc
     6841 aggtcgaact cgaccggggc gccgagcgct ttagccttct cggacggaag catcgacgcg
     6901 acgagaggaa tggcgccggt gacggcggcc gcgccgccgg cggccgcggt gagcgcgatg
     6961 atgttgcgcc gtccgcggtt cgtcgtttct ggggaatcgc tcatcgggag ggaccttgct
     7021 tttggaaggg cttgttcaaa aaggcgaaat tatacagaaa ggcccgggtc ctagacaaat
     7081 tttttgcccc aagtctatga tgcacaaagg gttttccatg atccaccccg gcgctttcgc
     7141 gcccggcgcc tttgggcaat aatccatgcg gtctttccac ccagctctcc gaaccgcaat
     7201 cccgacgatg cgcaggctct ggctgatctt cgcccaagcg acgacagtgg ccctcggcgc
     7261 gctcttcgtc gtcgtgacgc tgaagcccga gtggctgggc cggccgggcg gcacggaggt
     7321 cgttgagctg acgcaggccg tgcccgcggg tgcaggcagc ccgcgctcga gctcgtacag
     7381 cgacgcggtg aggcgcgcga cgccggcggt ggtcaacatc ttcaccagca aggaagtgaa
     7441 gaccccgccg cacccttttc tgaacgaccc gctctttcgc cacttcttcg gcgaccgcgc
     7501 cgatcccgaa tcgcagcgcg cggcgagtct cggctcgggc gtgatcgtca gcacgcgcgg
     7561 ctacattctc accaaccatc acgtggtcga ggcggcggac gagatcgagg tcgcgctcgc
     7621 cgatggcaag aagctgaagg cgcggctcgt cggctcggac cccgagaccg acctcgccgt
     7681 gctgcaggtg agcaatggca cgctgcccgc gatcaccttc ggcgacaccg agtccctgcg
     7741 cgtcggcgac gtcgtgctcg cgatcggcaa tccgttcggc gtcgggcaga cggtgacgat
     7801 gggcatcatc agcgcactcg ggcgcaacca gctcggcatc aacacattcg agaatttcat
     7861 ccagaccgac gccgcgatca atcccggcaa ctcgggcggc gcgctgatcg acgcggcggg
     7921 caacctgatc gggatcaaca cggcgatcta ctcgcgctcg ggcgggtcgc tcggcatcgg
     7981 gttcgcgatc ccggcgtcga gcgcgaagca ggtcatggag cagatcgtcg cgtccgggtc
     8041 ggtgacgcgc ggctggatcg gcgtagaggc ccaggagctg acgccggacc tgcctggcgg
     8101 cgcggacgcg aacggcgtcc tcatcgccgg cgtgctccgc ggcggtccgg ccgacaaggc
     8161 gggattccgg cccggcgacg tcctgatcgc gatcgacggg aagcaggtga aggacccgag
     8221 cgcgatgctg aacgcggtgg cggcgctcgt ccccggccgg accgccgcgg tgcgcctgcg
     8281 gcgcgacgag aagaatatcg acgtgaacgt cgaagtcggc aggcgtccgc ctccgaccct
     8341 gcgccgctga ggcgctattt ctcgacctcg ggctcctcga gacgcggcgc gtccgccgcc
     8401 gcgggccgac ccaggaagcg cgcgagcacc agtcccacct cgaagagcag ccaaagcggc
     8461 accgcgagca tcacctgcga cagcacgtcg ggcggcgtga agatcgcgcc gacgacgaat
     8521 gcgccgacga tcacgtagcc gcggatcgag cgcagcttgg cgagttcgac cacgccgagg
     8581 tgcacgagca cgacgacgac caccggtact tcgaaggtga tcccgaaggc g
//
LOCUS       DATDMW010000051         6208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1880868, whole genome shotgun sequence.
ACCESSION   DATDMW010000051 DATDMW010000000
VERSION     DATDMW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880868"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VI319_02240"
     CDS             <1..307
                     /locus_tag="VI319_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862692.1"
                     /translation="PIACGEAYNPPQGAYNKKYPLPKNGSARSGAAMAETPPAVQCPS
                     CGSADVAPVSLWLIPAQYYECRGCKVTFRDSRSAQEPPDVGRFNDGWAVPPDKPKSG"
     gene            complement(304..519)
                     /locus_tag="VI319_02245"
     CDS             complement(304..519)
                     /locus_tag="VI319_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862693.1"
                     /translation="MAGWVRGVACPSCFKALYTLEREDGQTEWKHKGATLQWDALGHF
                     VRCPDCNMRIPHRVAMDVPGFGFEVAR"
     gene            complement(627..842)
                     /locus_tag="VI319_02250"
     CDS             complement(627..842)
                     /locus_tag="VI319_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862694.1"
                     /translation="MSVQQNDEPIVVWTWRAKLPADSRKTPCWREVTQKMDRETARRF
                     AESEGIDFDRDMYAVPGSEEIRRHFDG"
     gene            980..1216
                     /locus_tag="VI319_02255"
     CDS             980..1216
                     /locus_tag="VI319_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862695.1"
                     /translation="MVSFVRGTFCPRCTSLVYRLERAENAASWIHIGAGLASDGEGWF
                     VACPSCRRRISLRPAPHLPGWGFEVDTRAPVLRE"
     gene            complement(1228..1878)
                     /locus_tag="VI319_02260"
     CDS             complement(1228..1878)
                     /locus_tag="VI319_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6862696.1"
                     /translation="MIRIVIADDHTIVREGLKQILAAASDFSVTGEAQNGHEVLARVR
                     ELDFDVLLLDMSMPGKSGTELIKQVHAEKPKLRILVLSMHEEEQYAVRAIKAGAAGYL
                     TKESASAQLVSAIRKVAGGGAYITSSVAEQLALGAMPDAAGPPHASLSDREFQVFRAI
                     VAGKTVTDIADELSLSVKTVSTHKARILEKMNLHNTADLVRYAMKNGLVDDPDKAS"
     gene            complement(1875..3581)
                     /locus_tag="VI319_02265"
     CDS             complement(1875..3581)
                     /locus_tag="VI319_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY6862697.1"
                     /translation="MPDDTSRDDPRGAHGLMHAAFGPLLAVGIFAALLALLAGLFGWY
                     THEMLATAGGLGFAAALLVSFLIYRQRGERHAAAAALREAEARIGGIVDSAMDAIVAV
                     DEEQRIVLFNAAAERVFRWPQGAVLGQPLGMLIPERLRAAHAGHIERFAATGVTSRRM
                     GAQTVLVGLRSTGEEFPIEASISQHAESGRKTFTVILRDITERVKSQQELARGEARLR
                     GILDSAMDAIITVDDTQHIVLFNRAAEQVFGCPRDQAIGAPLSWFIPERYRAGHHEHV
                     RRFGETGTSSRRMGGLRIVTGLRRNGEEFPIDASISQIDDNGARFYTVILRDVTERVR
                     GEEALASSREELRELAQAANSVREQEKSRIARELHDELAQALTALKMDVNWVAERAQT
                     QNDDPLEEKLAAMQSMLDDTVAATRRISADLRPLMLDDLGLGPAVEWLVQGFGERSGI
                     RCKLDIRAPEDLELQEPHASAVFRILQESLTNVAKHAKASLVQVSLARANGTIVLLVV
                     DNGRGFSTGDPRKPNSFGLMGLRERAYALGGEVRIDSGPGRGTRIDVRIPLPDTEMRE
                     AP"
     gene            3697..5082
                     /gene="hemN"
                     /locus_tag="VI319_02270"
     CDS             3697..5082
                     /gene="hemN"
                     /locus_tag="VI319_02270"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY6862698.1"
                     /translation="MSAKLVIDPDLIRKYGISGPRYTSYPTADRFVEAFGAEAYRYWL
                     GNRNIGGFSRPLSLYVHLPFCDTICYYCACNKIVTRDHGRSARYVEYLEREARLVAAE
                     MGDDRSVSQLHWGGGTPTFLADAEMAALMRMLATHFDLAVDGEYSIEVDPRKVTPERI
                     ALLAELGFNRISLGVQDFDPAVQRAVNRVQSVAETEGIMRAARLHGFRSVNVDLIYGL
                     PRQTLEGFSRTLEQVVESEPDRIALYSYAHLPTVFKPQRRILDADLPTGEAKLALLAR
                     AIDFLQSQGYVYIGMDHFARPGDDLAVAQRQGRLTRNFQGYSSAGDADIVGLGVSAIG
                     KLGAAYSQNVKELEPYYALLDDGRLPVLRGVELSADDLVRRAVIQALACQFEVSKESI
                     SIAHLVDFDEYFAPEIEMLRQHEREGLVCMDDEWITVTPQGRLLVRVLCMAFDKYLWA
                     AQRRAQYSKVI"
     gene            5171..>6208
                     /locus_tag="VI319_02275"
     CDS             5171..>6208
                     /locus_tag="VI319_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY6862699.1"
                     /translation="MEKRTQFTVWYVIIAVIGVLWLRDLWVTATQVQPIPYSEFQQQL
                     KDGKIAEIAISDNLIQGSYKDAAKDGRKRFVTTRVDPELAKDLSQYDVKFTGVVESHF
                     MRDVLSWVVPALVFFAVWMFFARRLAEQGGMGGGFLSVGKSKAKVYVETDTRVTFADV
                     AGVDEAKDELAEIIGFLKDPGRYSRLGGRVPKGVLLVGPPGTGKTLLARAVAGEANVP
                     FFSISGSEFVEMFVGVGAARVRDLFDQARKRAPAIIFIDELDALGRARGAYGVGGHDE
                     KEQTLNPLLVELDGFDASKGLVVLAATNRPEILDPALLRPGRFDRRVAVQPPDRLGRR
                     QILEVHTRSIPL"
BASE COUNT          942 a         2153 c         2113 g         1000 t
ORIGIN      
        1 tccgatagca tgcggcgagg cgtacaatcc gcctcagggg gcgtacaaca agaagtatcc
       61 gctgccgaaa aacggcagcg cgagaagtgg agcagcaatg gcggaaacac cgcctgcggt
      121 tcagtgcccc tcgtgcggct ctgccgacgt cgcgccggtt tccctgtggc tgattccggc
      181 ccagtactac gagtgccggg gctgcaaggt cacctttcga gactcccggt ccgcgcagga
      241 gcctcccgac gtggggcgct tcaacgacgg ctgggccgtt ccgcccgaca aaccgaagtc
      301 gggctagcgc gcgacctcga agccgaagcc gggaacgtcc atggcgaccc ggtgcgggat
      361 acgcatgttg cagtccgggc agcgcacgaa gtgaccgagc gcgtcccatt gcagggtcgc
      421 gcccttgtgc ttccactcgg tctgcccgtc ttcgcgctcc agtgtgtaga gcgccttgaa
      481 gcacgacgga cacgccacgc ctcgaaccca tccggccatt cttcttcccc gggcaagggg
      541 aagtccaata tacgccgttc ggcgccgatc gagctcgttg ctattttttc gcagcggctt
      601 gcgatttttt cgcagccgcg cgccggctag ccgtcgaagt ggcggcggat ctcctcggag
      661 cccgggacgg cgtacatgtc ccggtcgaag tcgatcccct cgctttcggc gaagcggcgc
      721 gcggtctcgc ggtccatctt ttgcgtgacc tcgcgccagc agggggtctt ccgggaatcg
      781 gcgggaagct tcgcccgcca cgtccatacc acgatgggct cgtcgttctg ctgcacgctc
      841 atgtcagaac attgtaaggt ctgcgcgctc gtttcccgat aggggaaacc ctaaaagcgg
      901 acgcgatcgt ttcacaaaac tgtaatggac ctgtaatgca gcgaacgtag agtcgcccgc
      961 accttgcggg agggacccca tggtgagctt cgtgcggggc acgttttgcc cgcggtgtac
     1021 cagtctcgtc taccggctgg agcgggccga gaacgcggct tcgtggatcc acatcggcgc
     1081 cggcctcgcc tcggacggcg agggctggtt cgtcgcgtgc ccgagctgcc ggcgccgcat
     1141 cagcctcagg cccgcgccgc atcttcccgg ctggggattc gaggtcgata cccgcgcgcc
     1201 cgtgctgcgc gagtagcggt cctcgcctca ggaggccttg tccgggtcgt cgaccaggcc
     1261 gttcttcatc gcgtagcgaa cgagatcggc cgtgttgtgc aggttcatct tctcgaggat
     1321 gcgcgccttg tgcgtgctca ccgtcttcac cgacaggctc agctcgtcgg cgatgtcggt
     1381 gacggtcttg ccggcgacga tggcccggaa cacctggaac tcgcggtcgg acagcgaggc
     1441 gtgcggcggg ccggcggcgt ccggcatcgc gccgagcgcg agctgctcgg cgaccgagct
     1501 cgtgatgtag gcgccgccgc ccgcgacctt gcggatcgcg gagaccagct gcgccgaggc
     1561 gctctccttg gtgaggtagc ccgccgcgcc ggccttgatc gcgcgcacgg cgtactgctc
     1621 ctcctcgtgc atgctgagca cgaggatgcg caatttcggc ttctcggcgt gcacctgctt
     1681 gatcagctcg gtgccgctct tgcccggcat ggacatgtcg agcagcagca cgtcgaagtc
     1741 gagctcgcga acgcgcgcga gcacctcgtg gccgttctgc gcctcgccgg tcaccgagaa
     1801 atcgctcgcg gccgcgagga tctgcttcag cccctcgcgc acgatggtgt ggtcgtcggc
     1861 gatgacgatg cggatcacgg cgcctcccgc atttccgtat cgggcagcgg gatgcgcacg
     1921 tcgatgcgcg tgccgcggcc cggtccgctg tcgatgcgca cctcgccgcc gagggcgtag
     1981 gcgcgctcgc gcagccccat cagcccgaac gagttcggct tgcgcgggtc gccggtcgag
     2041 aagccgcggc cgttgtcgac cacgagcagc acgatcgtgc cgttcgcgcg ggcgaggctg
     2101 acttgcacca gcgacgcctt ggcgtgcttc gccacgttgg tcagcgattc ctgcaggatg
     2161 cggaagaccg cgctcgcgtg cggctcctga agctcgaggt cctcgggcgc ccggatatcg
     2221 agcttgcacc ggatgccgct gcgctcgccg aatccctgaa ccagccattc cacggccggg
     2281 ccgagcccca ggtcgtcgag catgagcggg cgcaggtcgg ccgagatgcg gcgcgtggcc
     2341 gcgaccgtgt cgtcgagcat cgactgcatc gcggcgagct tctcctcgag cgggtcgtcg
     2401 ttctgagtct gcgcgcgctc cgcgacccag ttcacgtcca tcttcagcgc ggtgagcgcc
     2461 tgcgcgagct cgtcgtgcag ctcgcgcgcg atgcggctct tctcctgctc gcgcaccgag
     2521 ttggccgcct gcgcgagctc gcgcagctcc tcgcgcgagc tcgcgagcgc ctcctcgccg
     2581 cgcacgcgct cggtcacatc gcgcaggatc accgtgtaga accgggcgcc attgtcgtcg
     2641 atctgggaga tcgaggcgtc gatcgggaac tcctcgccgt tgcgccgcag tcccgtgacg
     2701 atgcgcagcc cgcccatgcg gcgcgacgac gtcccggtct cgccgaagcg gcggacatgc
     2761 tcgtggtggc ccgcacggta gcgctccggg atgaaccacg agagtggggc gccgatcgcc
     2821 tggtcgcgcg ggcagccgaa gacctgctcg gccgcgcggt tgaagagcac gatgtgctgc
     2881 gtgtcgtcga ccgtgatgat ggcgtccatc gccgaatcga ggatgccgcg caggcgcgcc
     2941 tcgccgcgcg cgagctcctg ctggctcttc acgcgctcgg tgatgtcgcg aaggatcacc
     3001 gtgaaggtct tccggccgct ttcggcgtgc tgcgagatcg acgcctcgat cgggaactcc
     3061 tctccggtgg agcgcagccc gacgagcacg gtctgcgcgc ccatgcgccg ggacgtcact
     3121 ccggtcgcgg cgaaacgctc gatgtggccc gcgtgagcgg cgcgcagccg ctccgggatc
     3181 agcataccga gcggctggcc gagcaccgcg ccctgcggcc agcggaacac gcgctcggcc
     3241 gcggcgttga acagcacgat gcgctgctcc tcgtcgaccg cgacgatcgc gtccatcgcc
     3301 gagtcgacga tgccgccgat ccgcgcctcg gcctcgcgca gcgccgcggc cgccgcgtgc
     3361 cgctcgccgc gctgccggta gatcaggaac gacacgagca gcgcggccgc gaagcccagc
     3421 cctccggccg tggcgagcat ctcgtgcgtg taccagccga acagccccgc gagcagcgcg
     3481 agcagcgcgg cgaagatccc gaccgcgagc agcgggccga aggcggcgtg catcagtccg
     3541 tgcgcgccgc gagggtcgtc tctggaggtg tcgtcgggca tggggggtta gtcccccgcg
     3601 cggggttttc cgggaaaaat ttgacgcacg tcaaaccccg ccgaaaccgc ggccgcatag
     3661 tacggcatgc acgcgaggag caatgcgacc gtggaaatgt cagccaagct cgtcatcgat
     3721 ccggacctca tccggaagta cggcatcagc gggccgcgct atacgtccta tccgaccgcc
     3781 gaccggttcg tcgaggcctt cggcgcggag gcgtaccggt actggctcgg caatcgcaac
     3841 atcgggggat tctcccggcc gctgtcatta tacgtacacc tgccattttg cgacacgatc
     3901 tgctactact gcgcctgtaa caagatcgtg actcgcgacc acgggcgctc ggcgcggtac
     3961 gtcgaatacc tcgagcgcga ggcgcgcctg gtcgccgcgg agatgggtga tgatcgcagc
     4021 gtgtcgcagc tgcactgggg cggcggcacg ccgacctttc tcgccgacgc cgagatggcc
     4081 gcgctgatgc gcatgctggc gacgcacttc gacctcgccg tcgacggcga atactcgatc
     4141 gaggtcgatc cgcgcaaggt gacgcccgag cggatcgcgc tcctcgccga gctcggcttc
     4201 aaccgcatca gcctcggcgt gcaggatttc gacccggcgg tgcagcgcgc ggtgaaccgc
     4261 gtgcagagcg tcgccgagac cgagggcatc atgcgcgcag cgcggctgca cgggttccgc
     4321 tcggtcaacg tcgacctgat ctacggcctg cccaggcaga cgctcgaggg cttctcgcgc
     4381 acgctcgagc aggtcgtgga gagcgagccc gaccggatcg cgctctactc gtacgcgcac
     4441 ctgccgacgg tcttcaagcc gcagcggcgg atcctcgacg ccgacctccc gaccggcgag
     4501 gccaagctcg cgctgctcgc gcgcgcgatc gacttcctgc agtcgcaggg ctacgtctac
     4561 atcggcatgg atcatttcgc gaggcccggc gacgacctcg ccgtggcgca gcgccagggc
     4621 cgcctcacgc gcaacttcca gggctactcg agcgcgggcg acgccgacat cgtcggcttg
     4681 ggcgtctcgg cgatcggcaa gctcggcgcc gcgtacagcc agaacgtgaa ggagctcgag
     4741 ccctactacg cgctgctcga cgacggccgc ctcccggtgc tgcgcggcgt cgagctctcc
     4801 gccgacgatc tcgtgcgccg cgcggtgatc caggcgctcg cctgccagtt cgaggtgtcg
     4861 aaggagtcga tctcgatcgc ccacctcgtc gacttcgacg agtacttcgc gccggagatc
     4921 gagatgctgc gccagcacga gcgcgagggc ctggtgtgca tggacgacga gtggatcacg
     4981 gtcaccccgc agggccggct gctcgtgcgc gtgctgtgca tggcgttcga caagtacctc
     5041 tgggcggcgc agcgccgggc gcagtactcg aaggtgatct gaccgcaaaa aaagaccgcc
     5101 gcggccggcg aagcgccggc gcggccggaa cgaacccaac gggccgctgt ccctcccaga
     5161 cggagccccc atggaaaagc gcactcagtt caccgtctgg tacgtcatca tcgccgtgat
     5221 cggcgtgctc tggctgcgcg acctctgggt gacggcgacc caggtgcagc cgatccccta
     5281 cagcgagttc cagcagcagc tcaaggacgg gaaaatcgcc gagatcgcca tctcggacaa
     5341 cctcatccag ggcagctaca aggacgcggc caaggacggg cgcaagcgct tcgtcaccac
     5401 gcgcgtcgac ccggagctcg ccaaggacct gtcgcaatac gacgtcaagt tcaccggcgt
     5461 ggtcgagagc catttcatgc gcgacgtgct gtcgtgggtg gtgccggcgc tcgtgttctt
     5521 cgcggtctgg atgttcttcg cgcggcgcct cgccgagcag ggcgggatgg gcggcggctt
     5581 tctctcggta ggcaagagca aggccaaggt ctacgtcgag accgacaccc gcgtcacctt
     5641 cgccgacgtc gccggcgtcg acgaggcgaa ggacgagctg gccgagatca tcggcttcct
     5701 gaaggacccg gggagataca gccgcctggg cggccgggtg ccgaagggcg tgctgctggt
     5761 cggcccgccc ggcacgggca agacgctgct cgcgcgcgcg gtcgcgggcg aggccaacgt
     5821 cccgttcttc tcgatctcgg gctccgagtt cgtcgagatg ttcgtcggcg tgggcgcggc
     5881 gcgcgtgcgc gacctgttcg accaggcgcg caagcgggct ccggcgatca tcttcatcga
     5941 cgagctcgac gcgctcggcc gcgcccgggg cgcctacggc gtcggcggcc acgacgagaa
     6001 ggagcagacg ctcaacccgc tgctggtcga gctcgacggc ttcgacgcga gcaaggggct
     6061 ggtcgtgctc gccgcgacca accgccccga gatcctcgac ccggcactgc tgcgcccagg
     6121 cagattcgac cgtcgcgtcg ccgtgcagcc acccgaccgg ctcggccgcc ggcagatcct
     6181 cgaggtacac acgcgctcga tcccgctc
//
LOCUS       DATDMW010000052         4984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2004620, whole genome shotgun sequence.
ACCESSION   DATDMW010000052 DATDMW010000000
VERSION     DATDMW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004620"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..536
                     /locus_tag="VI319_02280"
     CDS             153..536
                     /locus_tag="VI319_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY6862700.1"
                     /translation="MAGNTAPGFKKYPKHRLATRPAGVHVQVKLKGELIADTRDAVAL
                     EETPEGHVVAPVVYYIPRKDVKMDRLVRTSHTTHCPFKGDASYFSIENGPENAVWSYE
                     RPYDEMTAIGELLAFYPDKFETVTS"
     gene            complement(526..1257)
                     /locus_tag="VI319_02285"
     CDS             complement(526..1257)
                     /locus_tag="VI319_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY6862701.1"
                     /translation="MRTHTTLVINPKGGSGKTTVTTNIASYFAARGVPVAIMDYDPQS
                     SSLAWLQLRKPSCPRIHGADASPQRGMRLRGITMHVPADTRQLLIDAPAGAAGLLLQE
                     MLGRADSILIPVAPSAIDIHASANFIRDLLIAGRIRMRNVRIGVIANRVRGSMQAYPP
                     LERFLAVLNFPIVGRISDSDAYVRAAEAGVGVFEMDPAASEAEREELAPVCDWVERGR
                     EPGASGDKIVPLRPRPQGAISAFMK"
     gene            complement(1369..2127)
                     /locus_tag="VI319_02290"
     CDS             complement(1369..2127)
                     /locus_tag="VI319_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY6862702.1"
                     /translation="MSLPAFKIATWNVNGIRARQAQFCEWLEQDRPDVVCLQELKAEP
                     SQIPAECGHVDYHVYWHGLRAYSGVSLHIRKGLFETDPVFSHPEFDMESRIVQAQFGN
                     LVLASVYVPNGGKDYEAKLQFMLRLAEWTRQLHRDGRELVLCGDMNITRTERDVHPRE
                     RKPGIIGQREEERDLFDKLLGQHLVDVGRALDPDNDNMFTWWAPWRNMRQRNIGWRLD
                     YILASPSIAARAVSCPVLAQVGTSDHAPVVMTTG"
     gene            complement(2124..2885)
                     /locus_tag="VI319_02295"
     CDS             complement(2124..2885)
                     /locus_tag="VI319_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6862703.1"
                     /translation="MNVHAIRAIYGFEMARTRRTLMQSIISPVISTALYFVVFGAAIG
                     GRIPQVEGVSYGAFIVPGLIMLSLLTQSISNASFGIYFPKFVGTIYELLSAPVSYLEI
                     VVSYVGAAATKSVILGLVILATAALIVPLRIEHPVWMVVFLLLTSITFSLFGFIIGIW
                     ADGFEKLQLIPLLIVTPLTFLGGSFYSIAMLPPFWQKVSLFNPVVYLVSGFRWSFFGL
                     ADVGVAVSLGMTCVFLAATLAVVAWIFRTGYRLRT"
     gene            complement(2891..3823)
                     /locus_tag="VI319_02300"
     CDS             complement(2891..3823)
                     /locus_tag="VI319_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862704.1"
                     /translation="MTPARPAISIANLSKTYASGFGALRNLSLEIRPGEIFALLGPNG
                     AGKTTLIGCVCGLVNPSEGTIRVDGHDIVSEYRAARSLIGLVPQELTTDAFESVWNTV
                     SFSRGLFGRSPDPAHIERVLKDLSLWDKRNNKVMTLSGGMKRRLLIAKALSHEPRILF
                     LDEPTAGVDVELRQAMWNLVRRLKAEGVTIVLTTHYIDEAEEMADRVGVINKGELILV
                     EDKASLVRKLGKKQLTLHLDHPLERVPEALAGYNLELVPGGADLVYTYDERGDRAGIT
                     ALLQALPQAGVRFKDLQTRQTSLEDIFVTLVRRT"
     gene            complement(3820..4581)
                     /locus_tag="VI319_02305"
     CDS             complement(3820..4581)
                     /locus_tag="VI319_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862705.1"
                     /translation="MGFSGTYADREKDNVAQPVLERNKEGKFECGTADSYNVCEWASI
                     DRLERGRRGGHIAALRACVAAQAILSFDEKLQTPRDMADVPVVVQEWTGSHYTTLQML
                     ESAVGRDHVNIAKGGLPHRRWEALKDGSARAVTVMEPFISLGVKEGAHIVAASFYRGG
                     EVIAPELSTEQRKAYYDALNAAVDLINGDFYKYAHHVVAHAKGALKPNELSRAFVHYK
                     HVDYYDPVLFRRAYDWMKDRGMTEGQSEHAALVVG"
BASE COUNT          814 a         1658 c         1719 g          793 t
ORIGIN      
        1 cgctcgccgc aaagagcggg ttcgagctgg tgaacgtcgg cgtcggaccg ggcatggacc
       61 agttcatgaa ggagaaagcc gcgctctaca tggagagcgc gaagctgctc ggcctggtga
      121 agtagcccgg cgcggaattc aggaggacct ccatggctgg caacaccgct cccggtttca
      181 agaagtaccc caagcaccgc ctcgcgacgc gccccgcggg cgtgcacgtg caggtgaagc
      241 tgaagggcga gctgatcgcg gacacgcgcg acgcggtcgc gctcgaggaa acgcccgagg
      301 gtcacgtggt ggcgccggtc gtctactaca tcccgcgcaa ggacgtgaaa atggatcgcc
      361 tggtgcgcac gagccacacg acgcactgcc cgttcaaggg cgacgcgtcc tacttctcga
      421 tcgagaacgg acccgagaac gcggtgtgga gctacgagcg gccgtatgac gagatgaccg
      481 cgatcgggga gctgctggcc ttctatcccg acaagttcga gacggtcact tcatgaacgc
      541 cgagatcgcg ccctgcggac gcggccgcag cggcacgatc ttgtcgcccg aagcgccggg
      601 ctcgcgcccg cgctcgaccc agtcgcacac cggggcgagc tcctcgcgct ccgcttcgga
      661 ggcggcggga tccatctcga acacgcccac ccccgcctcg gcggccctca cgtaggcgtc
      721 cgagtcgctg atccgcccga cgatcgggaa gttgaggacg gcgagaaagc gctcgagcgg
      781 cggataagcc tgcatcgagc cgcgcacccg gttcgcgatc acgccgatgc ggacgttgcg
      841 catgcggatg cgccccgcga tcagcaggtc gcggatgaag ttggcgctcg cgtggatgtc
      901 gatcgccgag ggcgccaccg ggatcaggat cgagtcggcg cgcccgagca tctcctgcag
      961 cagcaggccc gcggcgccgg ccggggcgtc gatcaggagc tggcgcgtat cggcggggac
     1021 gtgcatcgtg atgccgcgca ggcgcatgcc gcgctgggga gaggcgtcgg cgccgtggat
     1081 cctgggacac gaaggcttgc gcagctgcag ccaggcgagg ctggagctct gcgggtcgta
     1141 gtccatgatc gcgaccggca cgccgcgcgc cgcgaagtag ctggcaatgt tcgtcgtgac
     1201 ggtcgtcttg cccgatccgc ccttcggatt gatcaccagc gtggtgtgcg ttctcatgtc
     1261 ggcgcccccg ggtcttttcg tccctcccgc cgagaaggcg taggttacgc cagtacaccc
     1321 ggcctacgcc aatcccccgc ccgcggtccg ttaagtagtc ctcaaacctc agcccgttgt
     1381 catcacgacg ggcgcgtggt cgctggtgcc gacctgcgcg agcaccgggc agctcacggc
     1441 ccgggccgcg atcgagggcg aggcgaggat gtaatccagg cgccagccga tgttgcgctg
     1501 cctcatgttc cgccacggcg cccaccacgt gaacatgttg tcgttgtccg ggtcgagcgc
     1561 tcggccgacg tcgaccaggt gctggccgag gagcttgtcg aagagatcgc gctcctcctc
     1621 gcgctggccg atgatgccgg gcttgcgctc gcgcgggtgc acgtcgcgct cggtgcgcgt
     1681 gatgttcatg tcgccgcaga ggacgagctc gcggccgtcg cggtgcagct ggcgcgtcca
     1741 ctccgcgagc cgcagcatga attgcagctt ggcctcgtag tccttgccgc cgttcggaac
     1801 gtacaccgac gccaggacca ggttcccgaa ctgcgcctgg acgatgcgcg actccatgtc
     1861 gaactcggga tggctgaaga ccggatcggt ctcgaacagc cccttgcgga tgtgcagcga
     1921 cacgccggaa taggcgcgca ggccgtgcca gtacacgtgg tagtcgacgt gcccgcactc
     1981 ggcagggatc tgcgagggct cggctttcag ctcctgcagg cacacgacgt cgggccggtc
     2041 ttgctccagc cattcgcaga actgcgcctg gcgggcgcgg atgccgttca cgttccaggt
     2101 ggcgatcttg aatgcgggca ggctcatgtc cgcaggcggt accccgtcct gaagatccag
     2161 gcgaccacgg ccagggtcgc ggcgaggaac acgcaggtca tgccgaggct caccgcgacg
     2221 ccgacgtcgg cgagcccgaa gaagctccag cggaagccgc tgacgaggta caccaccggg
     2281 ttgaacagcg acactttctg ccagaacggc ggcagcatgg cgatcgaata gaagctgccg
     2341 ccgaggaagg tgagcggcgt gacgatgagc agcgggatga gctggagctt ctcgaagccg
     2401 tcggcccaga tgccgatgat gaagccgaac aggctgaagg tgatggaggt gagcagcagg
     2461 aacacgacca tccacaccgg gtgctcgatc cgcagcggca cgatgagggc cgcggtcgcg
     2521 aggatgacga ggccgaggat gaccgacttg gtggcggccg cgcccacgta gctcaccacg
     2581 atctcgaggt acgacaccgg cgctgagagc agctcgtaga tcgtcccgac gaacttcgga
     2641 aagtagatgc cgaaagaggc gttcgagatg ctctgcgtga gcagcgacag catgatcagc
     2701 cccggcacga tgaacgcgcc gtaggagacg ccctcgacct gcgggatgcg cccgccgatc
     2761 gccgcgccga acacgacgaa gtagagcgcg gtcgagatca cgggcgagat gatgctctgc
     2821 atcagcgtgc ggcgcgtgcg cgccatctcg aagccgtaga tggcgcggat cgcgtggacg
     2881 ttcaggccgc tcacgtccgc ctcacgagcg tgacgaagat gtcctcgagc gaggtttgcc
     2941 tggtctggag gtccttgaag cgcacgcccg cctgcggcag ggcctggagc agcgcggtga
     3001 tgcccgcgcg gtcgccgcgc tcgtcgtacg tgtagacgag atcggcgccg cccggtacca
     3061 gctcgaggtt gtatccggcg agcgcttccg ggacgcgctc gagcggatga tcgaggtgca
     3121 gcgtcagctg cttcttcccg agcttgcgca cgagcgaggc cttgtcctcg accaggatca
     3181 gctcgccctt gttgatgacg ccgacgcgat cggccatctc ctcggcctcg tcgatgtagt
     3241 gcgtggtgag gacgatcgtg acgccctcgg ccttgagcct gcgcaccagg ttccacatcg
     3301 cctggcgcag ctcgacgtcg acgcccgcgg tcggctcgtc gaggaacagg atgcgcggct
     3361 cgtgcgacag cgccttggcg atgagcaggc gccgcttcat gccgcccgag agcgtcatca
     3421 ccttgttgtt gcgcttgtcc cagagcgaca ggtccttcag cacgcgctcg atatgcgcgg
     3481 gatcggggga gcgaccgaac aggccgcggc tgaaggagac ggtgttccac accgactcga
     3541 atgcgtcggt cgtcagctcc tggggcacga gcccgatcag cgagcgcgcc gcgcggtact
     3601 ccgagacgat atcgtgcccg tcgacccgaa tcgtcccttc cgacgggttc accaggccgc
     3661 agacgcagcc gatgagcgtg gtctttccgg ccccgttcgg tccgagcagc gcgaagatct
     3721 cgccgggacg gatctcgagg ctgaggttac ggagcgcgcc gaagcccgac gcgtacgtct
     3781 tcgaaagatt ggcgatcgag atcgccggcc gggcgggcgt cagccgacca ccagcgcagc
     3841 gtgctcgctc tgaccctcgg tcatgccgcg gtccttcatc cagtcgtagg cgcgacggaa
     3901 caggaccggg tcgtagtaat ccacgtgctt gtagtgcacg aacgcgcgcg agagctcgtt
     3961 cggcttgagc gcgcctttcg cgtgcgcgac gacgtgatgc gcgtacttgt agaagtcccc
     4021 gttgatcagg tcgaccgccg cgttcagggc gtcgtagtag gccttgcgct gctccgtgct
     4081 gagctcgggc gcgatcacct cgccgccgcg gtagaacgac gcggcgacga tgtgcgcgcc
     4141 ctccttgacg cccaggctga tgaagggctc catgacggtg accgcgcggg cgctgccgtc
     4201 cttcagcgct tcccagcgcc ggtgcggcag cccgcccttg gcgatgttga cgtggtcgcg
     4261 gccgaccgcg ctctcgagca tctgcagcgt ggtgtagtgc gagccggtcc actcctgcac
     4321 gaccaccggg acgtcggcca tgtcgcgcgg agtctgcagc ttctcgtcga agctgaggat
     4381 cgcctgtgcc gccacgcagg cgcgcagcgc cgcgatgtgg ccgccgcgcc tgccgcgctc
     4441 gaggcggtcg atgctcgccc actcgcacac gttgtagctg tcggccgtgc cgcactcgaa
     4501 cttgccttcc ttgttgcgct cgagcaccgg ctgcgcgacg ttgtccttct cgcggtcggc
     4561 gtacgtcccg gagaagccca cgtcgagccc cgccttcgcg aagaggcctt cctcgatgcc
     4621 gacgatgacg ggcaggtcga ataccgggtt gttcggggcg attttgacga cggggcgggt
     4681 cgcggcactc aggtcggtcc tcttcctggg cagataggcc gataggatac tgcgccagca
     4741 cccccgctcg caatccgccg tccgcgccgg cccggggacg gataaaatgc tcgctccccg
     4801 aagggagccc catggccaac atcaccccca ttgcccggga gccgatcgcc gacctcgccg
     4861 ccgccagccg gatcctcgcg gcccagggcg tggtcgacgg cttcggacac gtctcgatgc
     4921 ggcatccgca ggcgccgaac cgctatctga tggcgcgctc gctcgcgccg gcgctggtga
     4981 cgcc
//
LOCUS       DATDMW010000053         5475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_884637, whole genome shotgun sequence.
ACCESSION   DATDMW010000053 DATDMW010000000
VERSION     DATDMW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884637"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VI319_02310"
     CDS             complement(<1..1167)
                     /locus_tag="VI319_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862706.1"
                     /translation="MRLYATTVFLSAFLLFQVQPLVAKIILPWFGGSAAVWTVCMLFF
                     QVLLLLGYFYAHGAIRWQTSRRQRVVHWLLLAAALAAMPLSASETWKPHGGEDPTLRI
                     LGLLAATIGLPYLVLSTTGPLAQAWFARERPGASPYRLFALSNLGSMLALVSYPVLVE
                     PFAPVRVQTTAWSAGFAAFAVLCAALAWRASHADPHPQAGAADAPAPRPVTMLLWVAL
                     PACTSVLLLAFTSHMSLNIAAIPLLWVLPLALYLLSFVLSFDAPRWYRRTVWLPLLVL
                     GVAAVSYTLSAEHQNTDVWMLIPLYSATLLAACMACHGELARSRPHPRHLTAYYLLIA
                     FGGALGGLLVGLVAPHAFDDLYELPLGISALAFLVPAALLVGRTAWRPRRPLVLA"
     gene            1237..2304
                     /locus_tag="VI319_02315"
     CDS             1237..2304
                     /locus_tag="VI319_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6862707.1"
                     /translation="MHKKGAAVCAGVLLALLAVVRGSYAQDKAPEVYLYRGADRDSKL
                     VAAAKREGAISLYTSMQLPDSQPLTQAFEKKYGIRVSLWRASGEKIVQRAVTEARAGR
                     YDVDVVETDGAQMEALYREKLFAPFYSPSTADIPPEIIPAHRSYVPSRLTLYVLVYNT
                     KLVAPGDVPKTYEDLLDPKWAGKIGIEGADMPWFAAVVKAMGVDKGLAYFRKLAVNRP
                     SMRNGHTLLAEMVSSGELALAPDAHVQGVARLKARGAPIDWKPLQPAFGQPSSVAVTR
                     RAPHPNAALLFADFILSREGQEIIKARKRVPSSRAVNSPLNQFEYRLIDPVIVLDEWD
                     VWEKRFSALFLRGQRIRKESE"
     gene            complement(2312..3091)
                     /locus_tag="VI319_02320"
     CDS             complement(2312..3091)
                     /locus_tag="VI319_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6862708.1"
                     /translation="MDLGIRGKKALVCGASKGLGRGCAVSLAREGCVLTIVARGREAL
                     EKAAGDIRATTGASVTTVAVDITTAEGRAAALAACPQPDILVTNAGGPPPGDFRKFGR
                     DEWIRALEANMLTPIELIKATVDGMIARKFGRIVNITSSAVKAPIDILALSNGARSGL
                     TGFVAGLARATVKHNVTINNLLPGFFDTDRQQATLGQGAKDRGISLEEMRRLKVSQIP
                     AGRMGDPAEFGEACAYLCSAQAGYVTGQNFLVDGGAYPGTF"
     gene            complement(3127..4023)
                     /locus_tag="VI319_02325"
     CDS             complement(3127..4023)
                     /locus_tag="VI319_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6862709.1"
                     /translation="MSAARSYRAVGVAMALTGVACFSLRPILIKLAYVHVVDPVTLLA
                     LRMLFSLPFFVAAAAWAGRGERREPVSRRDAGAIVFLGFIGYYFSSFADFLGLQYISA
                     GLGRLILFLYPTIVVLLSLAFLRKRPSRRELLALVLTYSGVALVMWRAVGGANANLPL
                     GASLVFASAVGYAVYLVAGSQVVRRVGSLRFTAYAMSVASAFCIAQFLLLRPLSALEL
                     PRQVYGLAIAMALMSTVLPVFITSEALRRIGANQVALVGALGPVTTIFFGWVGLDERM
                     SPIQLAGAALVLFGVLLVSLRR"
     gene            complement(4023..>5475)
                     /locus_tag="VI319_02330"
     CDS             complement(4023..>5475)
                     /locus_tag="VI319_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY6862710.1"
                     /translation="ANRHGLIAGATGTGKTVTLQTLAQGFSRAGVPVFVSDVKGDLAG
                     LARPGADSPRLRERLELLGLRDFAFSSNPVVFWDVYGRQGHPARATVTDMGPLLLGRM
                     LALNDVQQGVLTLVFKIADDHGLLLLDMKDLRAMLQHVGEGAREYTTEYGNISGASIG
                     AIQRGLLELEHQGGEQLFGEPALHIADLMRTEDGKGVVNILAAEKLMSAPKLYSTFLL
                     WLLSELFEHLPEVGDPEKPKLVFFFDEAHLLFSDAPRALLEKIEQVVRLIRSKGVGVY
                     FVTQNPLDVPESVLGQLGNRVQHALRTFTPRDQKAVRAAADTMRPNPKLDVQAAITEL
                     SVGEALVSFLDDRGRPSMVERAFVVPPSSRLGPISDTERDAVIKLSALYGRYETPVDR
                     ESAYEKLKGRANDRQAPPPAPAGAPDATGGKGLLDTIGGILVGQTGPRGGRRDGLLQA
                     MAKSTARTIGSQLGREVVRGVLGSILGSGRKRR"
BASE COUNT          925 a         1869 c         1937 g          744 t
ORIGIN      
        1 cgcgagcacg agcggccgcc gcggtcgcca ggcggtgcgg ccgacgagca gcgcggccgg
       61 cacgagaaag gcgagcgccg aaattccgag cggcagctcg tacaagtcgt cgaaagcgtg
      121 cggtgcgacc agcccgacga gcagcccgcc cagcgcgccg ccgaatgcga tcagcaggta
      181 atacgccgtg aggtgccgcg ggtgcgggcg gctgcgcgcg agctcgccgt ggcaggccat
      241 gcacgccgcg agcagcgtgg cgctgtacag tgggatgagc atccacacgt cggtgttttg
      301 gtgctcggcg ctgagcgtgt aggacaccgc cgccacgccg agcacgagca gcggcagcca
      361 cacggtgcgg cggtaccacc tcggcgcgtc gaagctcagc acgaaactga gcagatagag
      421 cgcgagcggc aatacccaca ggagtgggat cgctgcgatg ttgagcgaca tgtgactggt
      481 gaacgcgagc agcaggaccg acgtgcacgc gggcagcgcg acccacagca gcatcgtcac
      541 cggccggggc gccggagcgt ccgccgcgcc ggcctgcggg tgcggatccg cgtgcgacgc
      601 gcgccacgcg agcgcggcgc acagcaccgc gaaggccgcg aagccggccg accaggccgt
      661 ggtctgcacg cgcaccggcg cgaacggctc gaccagcacc gggtagctca ccagggcgag
      721 catcgatccg aggttcgaca gcgcgaacag gcggtagggc gacgcgcccg gccgctcgcg
      781 cgcgaaccac gcttgcgcca gcgggcccgt cgtcgaaagc acgagataag gcaggccgat
      841 ggtcgcggcg agcagcccga ggattcgcag cgtcggatcc tcgcccccgt gcggcttcca
      901 cgtctcgctc gccgagagcg gcatcgcggc gagcgccgcg gcgagaagca gccagtggac
      961 gacccgctgc cgacgggacg tctgccagcg tatcgcgccg tgcgcgtaga agtagccgag
     1021 gaggaggagc acctggaaga agagcatgca cacggtccat acggcggccg atccgccgaa
     1081 ccacggcaga atgatcttcg cgaccagcgg ctggacttgg aagagcagga acgccgagag
     1141 aaagacggtg gtggcgtaca ggcgcatatt tttttgtgat cgagtttatc atctatggtt
     1201 tgcggcccgc tgacgggtgc cgccgaggaa gaggctatgc ataagaaagg agccgcagtc
     1261 tgcgcggggg tcctgctcgc gctgctcgcc gtcgtgcggg gatcgtatgc ccaggacaag
     1321 gcgccggagg tctatctcta tcgcggcgcc gatcgcgact cgaagctggt cgcggcggcg
     1381 aagcgcgagg gcgcgatctc gctctacacc tcgatgcagc tccccgactc gcagccgctc
     1441 acccaggcgt tcgagaagaa gtacgggatc cgcgtgtcgc tgtggcgcgc gagcggcgag
     1501 aagatcgtcc agcgcgccgt caccgaagcg cgcgcgggtc gctacgacgt cgacgtcgtg
     1561 gagaccgacg gcgcgcagat ggaggccctg taccgcgaga agctgttcgc ccccttctac
     1621 agcccgtcga ccgccgacat cccgcccgag atcatcccgg cgcacaggag ctacgtcccg
     1681 agccggctga cgctgtacgt gctcgtctac aacaccaagc tggtggcgcc gggcgacgtg
     1741 ccgaagacct acgaggatct gctcgatccc aagtgggccg gcaagatcgg catcgagggc
     1801 gccgacatgc cgtggttcgc cgcggtggtg aaagcgatgg gcgtcgacaa gggcctcgcg
     1861 tacttccgga agctcgcggt caaccgcccg agcatgcgca acggccacac gctgctcgcc
     1921 gaaatggtct cctcgggcga gctcgcgctc gcgcccgacg cccatgtcca gggcgtggcg
     1981 cgcctgaagg cgcgcggggc gccgatcgac tggaagccgc tgcagccggc gttcggccag
     2041 ccgagctcgg tcgcggtgac gcggcgcgcg ccgcacccga acgcggctct gctctttgcc
     2101 gacttcatcc tgtcgcgcga ggggcaggag atcatcaagg cgcgcaagcg cgtgccctcg
     2161 agccgtgccg tgaacagccc gctcaaccag ttcgagtacc ggctgatcga cccggtgatc
     2221 gtgctcgacg aatgggacgt ctgggaaaag cgcttctcgg cgctcttcct gcgggggcag
     2281 cggatccgca aggagtcgga gtaggccggg actagaaagt cccgggatac gcgcccccgt
     2341 ccacgaggaa gttctgcccg gtgacgtagc ccgcctgcgc gctgcacagg tacgcgcacg
     2401 cctcgccgaa ctcggccgga tcgcccatgc gaccggccgg gatctggctc accttgaggc
     2461 ggcgcatctc ctccaggctg atgccgcggt ccttggcgcc ctggccgagc gtcgcctgct
     2521 gccggtcggt gtcgaagaag cccggaagca ggttgttgat ggtgacgttg tgcttcaccg
     2581 tcgcgcgcgc gagcccggcg acgaagcccg tgaggcccga gcgcgcgccg ttcgagagcg
     2641 cgaggatatc gatcggcgcc ttcaccgcgc tcgacgtgat gttcacgatg cggccgaact
     2701 tgcgcgcgat catgccgtcg accgtcgcct tgatcagctc gatcggcgtc agcatgttcg
     2761 cctcgagcgc tcggatccac tcgtcgcgtc cgaacttgcg gaagtccccg ggcggcggac
     2821 cgcccgcgtt ggtgacgagg atgtcgggct gcgggcaggc tgcgagagcc gccgcgcggc
     2881 cttccgcggt ggtgatgtcg acggccaccg tcgtgacgct cgcgcccgtg gtcgcgcgga
     2941 tgtcgccggc ggctttctcg agcgcctcgc ggccgcgcgc gacgatcgtc agcacgcagc
     3001 cctcgcgcgc gagcgagacg gcgcagccgc gtcccagacc cttgctcgct ccgcacacca
     3061 gcgccttctt gccgcgtatt ccgagatcca tcgtccgttc ctcgattgat attggtgaat
     3121 gtggcctcag cgcctgaggc tcaccagcag cacgccgaag agcaccagcg ccgcgcccgc
     3181 caattgtatc gggctcatgc gctcgtcgag cccgacccag ccgaaaaaga tcgtggtgac
     3241 cggccccagg gcgccgacca gcgcgacctg gttggcgccg atgcggcgca gcgcctccga
     3301 cgtgatgaac acgggcagca cggtgctcat gagagccatc gcgatcgcga ggccgtagac
     3361 ctgccgcggc agctcgagcg cggacagcgg ccgcagcaaa agaaactggg cgatgcagaa
     3421 ggcgctcgcc acgctcatcg catacgcggt gaaccgcagc gaccccacgc gccgcacgac
     3481 ctggctgccc gcgaccaggt agaccgcgta gcccacggcg ctcgcgaaca cgagcgaagc
     3541 gccgagcggc agattggcgt ttgcgccgcc gaccgcgcgc cacatcacga gcgccacgcc
     3601 agaataggtt agtacgagcg caagcagctc gcgccgcgaa gggcgcttac gcaggaaagc
     3661 gagcgacagc agcaccacga tcgtcggata caggaacagg atcagccgcc cgaggccggc
     3721 cgagatgtac tgcagcccga ggaaatcggc aaagctcgaa aagtaatagc cgatgaagcc
     3781 gaggaacacg atcgcgcccg cgtcgcgccg gctgaccggc tcgcgccgct ccccgcggcc
     3841 ggcccacgcg gcggccgcga cgaagaacgg cagcgagaac agcatgcgca gggcaagcag
     3901 cgtcaccggg tcgacgacgt ggacgtaggc gagcttgatg aggatgggcc gaagcgagaa
     3961 gcaagcgact ccggtcagcg ccatcgccac gccgacggcg cggtacgatc gggctgcgga
     4021 cactagcggc gcttccggcc ggagccgagg atcgagccca gcacgccgcg gaccacctca
     4081 cggccgagct gcgagccgat cgtgcgggcg gtgctcttcg ccatcgcctg cagcaacccg
     4141 tcgcgccggc ccccgcgcgg accggtctgg ccgacgagga tgccgccgat cgtgtcgagc
     4201 agccccttgc cgccggtcgc atccggggcg cccgcgggcg ccggaggcgg cgcctggcga
     4261 tcattggcgc gccccttcag cttctcgtac gcggattcgc ggtccaccgg cgtctcgtag
     4321 cggccgtaga gcgccgacag cttgatgacg gcgtcgcgct cggtgtcgct gatcggcccc
     4381 aggcggctcg acggcggaac gacgaaggcg cgctcgacca tgctcggccg gcccctgtcg
     4441 tcgagaaacg agacgagcgc ctcgccgacc gagagctcgg tgatcgccgc ctgcacgtcg
     4501 agcttcgggt tcggccgcat cgtgtccgcg gccgccctca ccgctttctg gtcccggggc
     4561 gtgaaggtgc gcagcgcgtg ctgcacgcgg ttaccgagct gtccgaggac cgactccggg
     4621 acgtcgagcg ggttctgggt cacgaagtag acgccgactc ccttggaccg gatgagccgc
     4681 accacctgct cgatcttctc gagcagcgct cggggagcgt cgctgaagag caggtgcgcc
     4741 tcgtcgaaga agaacacgag cttgggtttc tcggggtcgc ccacctcggg caggtgctcg
     4801 aacagctccg agagcagcca aagcaggaac gtggaataga gcttgggcgc gctcatcagc
     4861 ttctcggcgg cgaggatgtt gacgacgccc ttaccgtcct cggtgcgcat gagatcggcg
     4921 atgtgcagcg ccggctcgcc gaagagctgc tcgccgcctt gatgctcgag ctcgagcagc
     4981 ccgcgctgga tggcgccgat cgacgcgccc gagatgttgc cgtactcggt cgtgtactcg
     5041 cgcgcaccct cgccgacgtg ctgcagcatc gcgcgcaggt ccttcatgtc gagcagcagc
     5101 agcccgtggt cgtcggcgat tttgaacacg agcgtcagca cgccctgctg cacgtcgttc
     5161 aaggccagca tgcgcccgag caggagcggc cccatgtcgg tgaccgtcgc gcgcgccgga
     5221 tggccctgcc ggccgtagac gtcccagaac acgaccgggt tcgaggagaa ggcgaagtcg
     5281 cgcaggccga ggagctcgag gcgctcgcgg agcctgggcg aatcggcgcc ggggcgtgcg
     5341 aggccggcga ggtctccctt cacgtcggac acgaagaccg ggacgccggc gcgcgagaac
     5401 ccttgcgcca gcgtctgcag ggtgacggtc ttgcccgtgc ccgttgcgcc ggcgatcagg
     5461 ccgtgccggt tggcg
//
LOCUS       DATDMW010000054        10077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1010095, whole genome shotgun sequence.
ACCESSION   DATDMW010000054 DATDMW010000000
VERSION     DATDMW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010095"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /locus_tag="VI319_02335"
     CDS             complement(<1..2213)
                     /locus_tag="VI319_02335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY6862711.1"
                     /translation="MSRTLAYRIARSAVVLAACAIGIFSWSGAFAEPRFSFAGTPGRL
                     PKDVVPEHYALRLTPGGDRFDGEAEIDVEVRKTVAAIVLNAADLSFRSARLVDAAGVA
                     MTLRWSVGRDDGIVTLDPPAGAIPPGRYRLRIDYSGRIGRHPQGLYRIGYRAREHGRT
                     VEKTMLATQMEPVYARRLFPGWDEPVFRATFDITAVVDAGLTAVSNMPVARESRRPDG
                     RREVTFARSPSMATYLVALFVGEMDRVEDEVDGVRLGIYAAKGKLARARYALQATKEI
                     VRHYDDYFGERYALPKLDQVALPGGGGGAMENWGAIAYNEAWLLYAPATDSPRRRQQV
                     YGTIAHEVAHQWFGNLVTMAWWDNLWLNEGFASWMAARTLRHFHPEWGSDLRDALAKD
                     RALSEDARRTTHPIQTPVESDARAMDVFDAITYAKGAALLRMLESYLGEAAFRDGVRR
                     YIVAHRGSSATTADLWQALSEASGSDVAAVAAAWTEQPGAPVVRATRRCRDGAETLTL
                     SQERFALNDAKAAPLAWKIPVVVADAFGARRAILLERAPQEIALGRCAGLLVVNAGDT
                     GYYRVQYDDAALRRLAAGLPFLDAQDRLKLLADTFALVQAGRVDVTRYLALAERLGTE
                     TERAVWDQVIGSLAFVRDLMDDPADAEAMQAYTAHLLRTPFARVGWDRREKDSPETAL
                     LRRSLIDALGRADDPEVVSEAKARFAARRTQPIDPAIRAAVLDVVGRHADEAEFDA"
     gene            2235..2438
                     /locus_tag="VI319_02340"
     CDS             2235..2438
                     /locus_tag="VI319_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862712.1"
                     /translation="MRTILALLAAAALAGCGVETASTAATAAALKKQELEQGKKTMEQ
                     MQKSIGQAMEQESRRAQGADDDK"
     gene            complement(2443..2964)
                     /locus_tag="VI319_02345"
     CDS             complement(2443..2964)
                     /locus_tag="VI319_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6862713.1"
                     /translation="MSTPRLLAAALALACSGAFAAGPNLGKPITPAEIAPWDISIQPD
                     GAGLPPGGGTPAQGQKIFAQKCAACHGENARGGRNTTLVGGDKIVNMDSTRTIANFWP
                     YATTVFDYIRRAMPWPQPRSLSDNEVYALTAYILSENKLIGPDDSMNSETLPKVRMPN
                     RDGFILRFPDKIQ"
     gene            complement(2951..4243)
                     /gene="soxC"
                     /locus_tag="VI319_02350"
     CDS             complement(2951..4243)
                     /gene="soxC"
                     /locus_tag="VI319_02350"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEY6862714.1"
                     /translation="MDKDDLIPAAANGLLDRRAFLRGGAAVAAVTGYALVKPAAAEPL
                     KDEPWSRTPGETVGTYGLPSRYEKKVARTLSNPNGEPRTQHARTPHQLLNGTFTPNGL
                     HFVISHAGNPDIDPERHKLVIHGLVKRPLVFTLDALERYPMVSRMTFVECGGNSAPLF
                     AHDPIQATVQALHGLSSCAEWTGVPLSTLLEETGIDARAKWLIAEGADAPALSRSVPL
                     KKGLDDAMIALYQNGERLMPGNGYPMRLLLPGWEGNMNVKYLRRIKLVDEPAMSYYEA
                     RTYAPILPNGKAYRFYFVNEVKSFITHPSFGHLLRGPGLYEISGLAWSGDGRIAKVLV
                     SADGGSSWAQAALQDPVLPKAFTRFRALWRWDGGPAVLQSRAWDEAGNVQPTRARIVA
                     ERGQSARTPPATAFISQHYNGPTSWAVDPKGEVKHVYA"
     gene            complement(4258..6360)
                     /locus_tag="VI319_02355"
     CDS             complement(4258..6360)
                     /locus_tag="VI319_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY6862715.1"
                     /translation="MSLCRCLALIALLLPPGLSGAADLSGVVTGAGGPEAGVWVIAET
                     NGLPTKFAKIVVTDDHGRYLVPDLPEATYDVWVRGYGLVDSPKVKASPGRRLDLRAVP
                     APSPAAAAEYYPAIYWYSMLKIPEKSRFPGTGEKGNGVPETFHTQGEWLNVVKTNGCN
                     SCHALGTKGTRTIPKALGSFPSSFDAWARRIASGQAMTTMTSVMGRLNPQLALSLFAD
                     WTDRIAAGELPRSAPRRPEGAERNLVLTLWDWSTPTGYMHDLIGTDKRKPTINAYGRL
                     YGSTEESTDDFPVLEPKTNTATRVHHPVRDPKTPSSKSAPMAPSPYWGEKPIWDSQSS
                     LHNPMMDERGRVWFTARVRPPENPAFCRKGSTHPSAQVVPLERANRHLSMYDPATGKF
                     TLISTCFPTHHLIFAEDADQTLWTSSGATASGVLGWLNRRVFEETGDEQRAQGWTPFV
                     LDTNGNGRRDDYVDAKAPLDPTKDKRVMIGLYAVAVNSQDGSVWGTSLGFPGYVVRVA
                     PGPDPTHTALTEIYEPPFPGYGPRGGDIDRNGVYWVSLSSGHLGEFDRRKCKVLNGPT
                     ATGKHCPEGWTLHPFPGPQFAGVSETGSVESSYFTWVDQFDAFGLGRNVPYATGNLNE
                     ALLALVDGKFVTLRVPYPNGFYTKWAEGRIDDPAAGWKGRALWATFGTRTVFHLEGGT
                     ANRPKVVRFQLRPDPLAH"
     gene            6449..7627
                     /locus_tag="VI319_02360"
     CDS             6449..7627
                     /locus_tag="VI319_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY6862716.1"
                     /translation="MPDSSRKRFLAEHAEPLYRRITRDYSRFLRVADVVYEAAELAPG
                     LVPTRAQVAAESARLQRDKQGLEIGQGVLLSAVLADPRAGRHLCHAMLLPRREALERL
                     PELERNGRVDLGAAEVVREGKASIVVQKNPRHLNAEDDTTLAAAETAVDLALLDPGTE
                     VCVLRGGPVQAGRHAGRRVFGSGINLTHLYYGRIPFLWYLERDLGVVSKIYRGLARED
                     APPDDVAGNTREKLWIAAVEGFAIGGHCQYLLVMDHVVAEESAYLTLPARKEGIIPGA
                     ANLRLARFVGDRIARQLVMAERRLDCASPEGRLVCDELCAPGDMDAAIARVVERLTSS
                     GVVGAIANRRGFRLAQEPLDRFREYMALYALEQAYCHFSPALIDNLERYWNAKERKPV
                     "
     gene            7672..7899
                     /locus_tag="VI319_02365"
     CDS             7672..7899
                     /locus_tag="VI319_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862717.1"
                     /translation="MKKMAIVLAVLALASAPAAASQPNRLERAAHKTGAALDRAAQKT
                     GHALKKAAVKTGQALEKAGRKTAKWIESKTS"
     gene            complement(7901..8704)
                     /locus_tag="VI319_02370"
     CDS             complement(7901..8704)
                     /locus_tag="VI319_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6862718.1"
                     /translation="MHQYGVRDVERLLNLSRSTIQSLVKAGFVSPARGPRRSLLFSFQ
                     DLIVLRTAQALAAAKVPARRITKSLKELRRQLPASVPLSGLSIGAVADRVVVKEGASR
                     WQADSGPYLLALEVEPAGGSLKVIERRGPSAEECFERALALERTDVPGARAAYERAVA
                     ADASHLSAHVNLGLLLHEDGRFAEAEAAYRRALSACGGDPVLWYNLGVLLEDMERKDE
                     ALAAYEEALRKNPQFADCHYNLALLCEDLNKPAKAIRHMAQYRRLVERR"
     gene            complement(8721..9617)
                     /locus_tag="VI319_02375"
     CDS             complement(8721..9617)
                     /locus_tag="VI319_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY6862719.1"
                     /translation="MAARSIASLSVSFGLVSIPVKLYSATEASRAISFNLLHKACGSR
                     LKQQYLCVKEEVVVPREDMVKGYEFAKDQYVMFSPEELKALEETGTHTAEITEFVPLE
                     SVDPVYFDKAYYLAPDKGGAKPYALLAKALRETGRCALGRWAARGKQYIVMLRPATLD
                     TGTGKADALVMQQLLYADEVRKIGEIEIPKTEVKDAELKLARQLIEQQASEAFDPAAY
                     KDDVKERIEAAVQKKVEGQEITMTEEAPQGGGQVIDLMEALRASLEKKGAVPSKGKEA
                     EARKPAKRAAAQAAPAKKAAKK"
     gene            complement(9684..>10077)
                     /locus_tag="VI319_02380"
     CDS             complement(9684..>10077)
                     /locus_tag="VI319_02380"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862720.1"
                     /translation="KTGLHVFVPIRRTIDFDTSRAVSELVGRHLMREHPKDITMEWSV
                     PKCTGKIFMDYNMNVRGKTLNVAYSPRGLPGAAVSMPLTWEELEKAHPLDFRITNAAE
                     RLVKTGDRWAEALTRKQSLEKVLENAKS"
BASE COUNT         1447 a         3501 c         3549 g         1580 t
ORIGIN      
        1 gcgtcgaact cggcctcgtc cgcgtgccgt cccaccacgt cgagaaccgc ggcccggatc
       61 gcgggatcga tcggctgcgt ccggcgcgcg gcgaagcgcg ccttcgcctc gctcaccacc
      121 tcgggatcat cggcgcggcc gagcgcgtcg atgagcgagc ggcgcagcag cgcggtctcg
      181 gggctgtcct tctcgcgccg atcccagccg acgcgcgcga acggcgtgcg cagcaggtgc
      241 gcggtgtatg cctgcatcgc ctcggcgtcg gccggatcgt ccatgagatc gcgcacgaac
      301 gcgagcgagc cgatgacctg gtcccagacg gcgcgctccg tctcggtgcc gaggcgctcc
      361 gcgagcgcga gatagcgcgt gacgtcgacg cgtcccgcct ggaccagcgc gaaggtgtcg
      421 gcgagcagct tcaggcggtc ctgcgcgtcg aggaagggca gcccggccgc gaggcggcgc
      481 agcgcggcgt cgtcgtactg cacgcggtag tagcccgtgt cccccgcgtt gaccacgagc
      541 agtcccgcgc agcgcccgag ggcgatctcc tgcggcgcgc gctcgagcag gatcgcgcgg
      601 cgggcgccga acgcgtcggc gacgacgacc gggatcttcc acgcgagggg cgcggccttc
      661 gcgtcgttga gggcgaagcg ctcctgcgac agcgtcagcg tctccgcgcc gtcgcggcag
      721 cgccgcgtcg cgcgcaccac cggggcgccc ggctgctcgg tccacgccgc ggccacggcc
      781 gcgacgtcgc tgcccgacgc ctcggacagc gcctgccaga ggtccgcggt ggtcgcgctc
      841 gagccgcggt gggcgacgat atagcgccgc acaccgtccc ggaaggcggc ctcgccgaga
      901 tacgactcga gcatgcgcag cagcgccgcg cccttggcat acgtgatggc gtcgaacacg
      961 tccatggcgc gcgcgtcgct ctccaccggc gtctggatcg ggtgcgtcgt gcgtcgggcg
     1021 tcctcggaca gcgcgcggtc cttcgcgagc gcgtcgcgca ggtcggagcc ccattcggga
     1081 tggaaatgcc gcagcgtgcg cgccgccatc cagctcgcga agccttcgtt cagccagagg
     1141 ttgtcccacc atgccatggt gacgaggttg ccgaaccact ggtgcgcgac ctcgtgcgcg
     1201 atggtgccgt agacctgctg ccgccggcgc ggcgaatcgg tcgcgggcgc atagagcaac
     1261 cacgcctcgt tgtaggcgat cgcgccccag ttctccatgg cgccgcctcc gccgccgggc
     1321 agcgcgacct ggtcgagctt gggcagcgcg tagcgctcgc cgaagtagtc gtcgtagtgg
     1381 cgcacgatct ccttcgtcgc ctgcagcgcg tagcgcgcgc gggcgagctt gcccttcgcg
     1441 gcgtagatgc cgagccgcac gccgtcgacc tcgtcctcga cccgatccat ctcgcccacg
     1501 aagagcgcga ccaggtacgt cgccatcgac ggcgagcgcg cgaacgtcac ctccctgcgg
     1561 ccgtcgggac gccgcgactc gcgcgccacc ggcatgttgg agacggcggt gaggccggcg
     1621 tcgaccaccg cggtgatgtc gaaggtggcg cggaacacgg gctcgtccca gccgggaaag
     1681 agccggcgcg cgtacacggg ctccatctgc gtcgcgagca tggtcttctc gaccgtgcgc
     1741 ccgtgctcgc gggcgcggta gccgatgcgg tacagcccct gcgggtgcct gccgatccgc
     1801 ccggagtagt cgatgcgcag gcgatagcgc cccggcggga tcgcgcccgc cggcgggtcg
     1861 agggtcacga tgccgtcgtc gcggccgacg ctccagcgca gcgtcatcgc cacgccggcc
     1921 gcgtccacca ggcgcgcgga tctgaagcta aggtccgcgg cgttgagaac gatcgccgcg
     1981 accgtcttgc gcacctcgac gtcgatctcg gcctcgccgt cgaagcggtc gcccccgggc
     2041 gtgaggcgca gcgcgtagtg ctcggggacg acgtccttcg gaagccggcc gggggtcccg
     2101 gcaaaggaga agcgtggctc ggcgaatgcc ccgctccagg aaaagatacc gatcgcgcag
     2161 gcggcgagaa cgaccgccga ccgggcgatg cggtacgcta gggtccggct catcggatcg
     2221 acagggagaa agccatgaga acgatattag ctttgcttgc ggccgccgcg ctcgccggct
     2281 gcggcgtcga gaccgccagc acggcggcca cggcggccgc gctcaagaag caggagctcg
     2341 agcagggcaa gaagaccatg gagcagatgc agaagagcat cggccaggcc atggaacagg
     2401 agagccggcg cgcccagggc gcggacgacg acaagtaagc cgctactgga tcttgtccgg
     2461 gaagcggagg atgaagccgt cgcggttcgg catccgcacc ttgggcagcg tctccgagtt
     2521 catcgagtcg tcggggccga tcagcttgtt ctccgacagg atgtacgccg tcagggcgta
     2581 gacctcgttg tcgctgagcg agcgcggctg cggccacggc atcgcgcgcc ggatgtagtc
     2641 gaacaccgtc gtcgcgtacg gccagaagtt ggcgatcgtg cgcgtcgaat ccatgttgac
     2701 gatcttgtcg ccgccgacga gcgtggtgtt ccgcccgccg cgcgcgttct cgccgtgaca
     2761 cgcggcgcac ttctgcgcga agatcttctg gccctgcgcg ggcgtcccgc cgccgggcgg
     2821 gagccccgcg ccgtcgggct ggatgctgat gtcccatgga gcgatctcgg cgggcgtgat
     2881 cggcttgccg aggttcggcc ccgccgcgaa ggcgccggag caggcgagcg cgagcgcggc
     2941 ggcgagcagc ctaggcgtag acatgcttca cctccccttt ggggtcgacc gcccagctcg
     3001 tgggcccgtt gtagtgctgg ctgatgaacg cggtcgcggg cggcgtcctc gcgctctggc
     3061 cgcgctcggc gacgatccgg gcgcgcgtcg gctgcacgtt gcccgcctcg tcccaggcgc
     3121 ggctctgcag caccgcgggc ccgccgtccc agcgccacag cgcgcggaag cgcgtgaacg
     3181 ccttcggcag caccgggtct tgcagcgcgg cctgcgccca gctcgagccg ccgtcggccg
     3241 agaccaggac cttggcgatg cggccgtcgc ccgaccacgc gaggcccgag atctcgtaga
     3301 gaccgggacc gcgcaggagg tgcccgaacg acgggtgcgt gatgaaggac ttcacctcgt
     3361 tcacgaagta gaagcggtac gccttgccgt tgggaaggat cggcgcgtag gtgcgcgcct
     3421 cgtagtagct catcgccggc tcgtcgacga gcttgatgcg gcgcaggtac ttcacgttca
     3481 tgttgccttc ccagcccggt agcagcagcc gcatcgggta gccgttgccc ggcatcagcc
     3541 gctcgccgtt ctggtagagc gcgatcatgg cgtcgtcgag ccccttcttc agcggcacgc
     3601 tgcgcgacag cgccggcgcg tcagcgccct cggcgatcag ccacttcgcc ctggcgtcga
     3661 tgccggtctc ctcgagcagc gtcgacagcg gcacgccggt ccactccgcg caggaggaca
     3721 gcccgtgcag cgcctgaacc gtcgcctgga tcgggtcgtg cgcgaacagc ggcgcgctgt
     3781 tgccgccgca ctcgacgaag gtcatgcgcg agaccatcgg gtagcgctcg agcgcgtcga
     3841 gcgtgaacac gagcggccgc ttcaccagcc cgtggatcac gagcttgtgc cgctcgggat
     3901 cgatgtcggg attgcccgcg tgcgagatga cgaagtgcag cccgttcggc gtaaacgtgc
     3961 cgttcagcag ctggtgcggc gtgcgcgcgt gctgcgtgcg cggctccccg ttcgggttcg
     4021 acagcgtgcg cgcgaccttc ttctcgtagc gcgagggcag gccgtaggtt ccgacggttt
     4081 cgccgggcgt gcggctccag ggctcgtcct tgagcggctc ggcggcggcc ggcttcacga
     4141 gcgcataccc ggtgaccgcc gcgacggcgg cgccgccgcg gagaaacgcg cgccggtcga
     4201 gaagaccgtt tgccgcggcg ggaatcaggt cgtccttgtc catcgtgcgt tcctccttca
     4261 atgcgccagc ggatccggcc gcagctggaa ccgcaccacc ttcggccggt tcgcggtgcc
     4321 gccttcgaga tggaataccg tgcgcgtgcc gaacgtggcc cacagcgcgc gccccttcca
     4381 gccggcggcc gggtcgtcga tgcggccttc ggcccacttg gtgtagaaac cgttcgggta
     4441 tggcacgcgc agcgtcacga acttgccgtc gaccagggcg agcagcgcct cgttcagatt
     4501 gccggtcgcg tacgggacgt tgcgcccgag cccgaacgca tcgaactggt cgacccaggt
     4561 gaagtagctc gactcgacgc tgcccgtctc ggacacgccc gcgaactgcg gcccggggaa
     4621 cgggtgcagc gtccagccct cggggcagtg cttgccggtc gcggtcgggc cgttcagcac
     4681 cttgcatttg cgccgatcga actcgcccag atgcccgctc gacagggaca cccagtacac
     4741 gccgttccga tcgatgtcgc cgccgcgcgg gccgtagccg ggaaacggcg gctcgtagat
     4801 ctcggtgagc gcggtgtggg tgggatccgg tcccggcgcc acgcgcacga cgtatcccgg
     4861 gaagccgagc gacgttcccc acaccgagcc gtcctgtgaa ttcacggcga cggcgtagag
     4921 cccgatcatg acgcgcttgt ccttggtcgg atcgagcggc gccttcgcgt cgacgtagtc
     4981 gtcgcgacgg ccgttgccgt tggtgtcgag cacgaacggc gtccagcctt gcgcgcgctg
     5041 ctcgtcgccc gtctcctcga acacgcgccg gttcagccag ccgaggacgc ccgaggcggt
     5101 cgcgccgctc gaggtccaca gcgtctggtc ggcgtcctcg gcgaagatca ggtggtgcgt
     5161 cggaaagcag gtgctgatca gcgtgaactt gccggtcgcc gggtcgtaca tcgacaggtg
     5221 ccggttcgcg cgctcgagcg gcacgacttg cgccgaggga tgggtcgagc ccttgcggca
     5281 gaacgcgggg ttctccggcg ggcgcacgcg cgccgtgaac cagacgcggc cgcgctcgtc
     5341 catcatcggg ttgtgcaggc tcgactggct gtcccagatc ggcttctcgc cccagtaagg
     5401 cgacggcgcc atcggcgcgc tcttcgacga aggcgtcttc ggatcgcgca ccgggtggtg
     5461 cacgcgcgtc gcggtgttcg tcttcggttc gagcacgggg aaatcgtcgg tgctctcctc
     5521 ggtcgagcca tagagcctgc cgtaggcatt gatcgtcggc ttgcgcttgt cggtcccgat
     5581 caggtcgtgc atatagccgg tcggagtgct ccagtcccag agcgtgagca cgaggttgcg
     5641 ctcggcgccc tccggtcggc gcggcgccga gcgcggcagc tcgcctgccg cgatgcggtc
     5701 ggtccaatcg gcgaagagcg agagcgcgag ctgcgggttc agccggccca tgaccgaagt
     5761 catcgtcgtc atcgcctgcc cggaggcgat gcgccgcgcc caggcgtcga acgacgacgg
     5821 gaacgagccg agcgccttcg ggatcgtgcg cgtgcccttc gtcccgagcg cgtggcagga
     5881 gttgcagccg ttcgtcttga cgacgttcag ccactcgcct tgcgtatgga aggtctcggg
     5941 cacgccgttg cccttttcgc ctgttcccgg gaaccggctc ttttccggga tcttcagcat
     6001 cgagtaccag tagatcgcgg ggtagtactc cgccgcggcg gcagggctgg gcgcgggtac
     6061 ggcgcgcaga tcgagacgcc tgcccggtga cgccttgacc ttcggcgagt cgaccagtcc
     6121 gtagccgcgg acccagacgt cgtaggtcgc ctccggaaga tccgggacga ggtagcggcc
     6181 gtgatcgtcg gtcacgacga tcttcgcgaa tttcgtcggg agcccattcg tctccgcgat
     6241 gacccagacg cccgcctcgg ggccgcccgc gccggtgacg acgccgctca agtccgccgc
     6301 gccggacagg ccgggcggca gcagcagcgc gatcagcgcg aggcaccggc aaaggctcat
     6361 cgtgtccgcc tcctcaggct gcgacgattc tagtccggcg accggcggcg cgcatgaccc
     6421 gtaatgcgcg ccgcgctatg ctgtgtcgat gccggattca tcgcgcaagc gcttcctggc
     6481 ggagcacgct gagccgctct accgccgcat cacgcgcgat tattcgcgct tcctgcgggt
     6541 cgccgacgtc gtctacgagg cggccgagct cgcgcccggt ctggtgccga cgcgcgccca
     6601 ggtcgccgcg gaatcggccc ggctgcagcg cgataagcag ggactcgaga tcggccaggg
     6661 cgtgctgctc tcggcggtcc tcgccgatcc gcgcgccggg cggcatctgt gccatgcgat
     6721 gctgctgccg aggcgcgagg cgctcgagcg gcttcccgag ctggagcgca acggccgcgt
     6781 cgacctcggc gccgccgagg tggtgcgcga gggcaaggcg tcgatcgtcg tccagaagaa
     6841 tccgcgccat ctcaacgccg aggacgacac gacgcttgcc gccgccgaga ccgccgtcga
     6901 cctcgcgttg ctcgatccgg gcaccgaggt ttgcgtgctg cgcggcggac cggtacaggc
     6961 cggccggcac gcgggccggc gcgtgttcgg gagcgggatc aatctcacgc atctctatta
     7021 cggcaggatc ccgttcctct ggtatctcga gcgcgacctc ggcgtcgtca gcaagatcta
     7081 ccgcggcctc gcgcgcgaag acgcgccgcc cgacgacgtc gccggcaata cgcgcgagaa
     7141 gctctggatc gcggccgtcg agggcttcgc gatcggcggg cactgccagt atctgctcgt
     7201 gatggaccat gtcgtcgccg aagagagcgc ctacctgacg ctgccggcgc gaaaggaggg
     7261 gatcatcccg ggcgcggcca acctgcgcct cgcgcgcttc gtcggcgacc gtatcgcgcg
     7321 ccagctggtg atggccgagc gccggctcga ttgcgcgagc ccggagggac ggctcgtctg
     7381 cgacgagctc tgcgcgcccg gagacatgga cgccgcgatc gcgcgcgtgg tcgagcggct
     7441 gacgagctcg ggcgtggtcg gcgcgatcgc gaaccggcgc gggttccggc tggcgcagga
     7501 gccgctcgat cggttccgcg aatacatggc gctgtacgcg ctcgagcagg catactgtca
     7561 tttcagcccg gcgctgatcg acaatctcga gcgttattgg aacgcgaagg agcggaagcc
     7621 ggtctgagtc tcagacggtg tggagcgcac cgtctccatg gagcgacacc gatgaagaag
     7681 atggcgatcg ttctcgcagt cctcgcgctc gcgtcggcgc cggcggccgc gagccagccc
     7741 aacaggctcg agcgcgccgc tcacaagacc ggcgcggcgc tcgaccgcgc agcgcagaaa
     7801 acgggacacg cgctgaagaa agccgcggtc aagaccggcc aggcgctcga gaaggccggc
     7861 cgcaagaccg cgaaatggat cgagagcaaa acgagctgag ctagcggcgc tcgaccaggc
     7921 gccggtactg cgccatgtga cggatcgcct tggcgggctt gttcaggtcc tcgcacagga
     7981 gcgcgaggtt gtagtggcag tcggcgaact gcgggttctt gcgcagcgcc tcctcgtacg
     8041 ccgccagcgc ctcgtccttg cgctccatgt cctcgagcag caccccgagg ttgtaccaga
     8101 gcacgggatc cccgccgcac gcggacagcg cccgccggta ggcggcctcg gcctcggcga
     8161 agcggccgtc ctcgtgcagg agcagcccga ggttgacgtg cgccgacagg tgcgacgcgt
     8221 cggccgcgac cgcgcgctcg tacgccgcgc gcgcccccgg gacgtcggtg cgctcgaggg
     8281 cgagcgcgcg ctcgaagcat tcctcggcgc tcggcccgcg gcgctcgatc accttgagcg
     8341 agccgccggc aggctcgacc tcgagcgcga gcaggtacgg tcccgagtcg gcctgccagc
     8401 ggctcgcgcc ttccttcacg accacgcggt cggcgaccgc tccgatcgaa agcccggaca
     8461 gcggaaccga ggccggaagc tggcgcctca gctccttcag cgatttcgtg atccgccgcg
     8521 ccgggacctt ggcggcggcg agcgcctgcg cggtgcgcag cacgatcaga tcctggaagg
     8581 agaagagcag gctgcggcgc gggccgcgtg cgggcgacac gaacccggcc ttcaccaggc
     8641 tctggatcgt gctgcgggaa agattgagca gccgctcgac gtcgcggacg ccgtactgat
     8701 gcatcgcctg cggccggaaa ttacttcttc gccgccttct tcgcaggcgc ggcctgggcg
     8761 gccgcgcgct tcgcgggctt gcgagcctcc gcctccttgc ctttcgaagg cacggcgcct
     8821 ttcttctcga ggctcgcgcg cagcgcctcc atcaggtcga tcacctggcc gccgccttgc
     8881 ggggcttcct ccgtcatcgt gatctcctgg ccctcgacct tcttctgcac cgcggcctcg
     8941 atgcgctcct tgacgtcgtc cttgtacgcg gcggggtcga aggcttcgga ggcctgctgc
     9001 tcgatcagct ggcgcgcgag cttcagctcg gcgtccttca cctcggtctt gggaatctcg
     9061 atctcgccga tcttgcgcac ctcgtccgcg taaagcagct gctgcatgac cagggcatcg
     9121 gccttgccgg tccccgtgtc gagcgtggcc ggccgcagca tcacgatgta ctgcttgccg
     9181 cgcgcggccc agcgcccgag cgcgcagcgc ccggtctcgc gcagcgcctt ggcgagcagc
     9241 gcgtagggct tggccccgcc cttgtcgggc gccaggtagt aggccttgtc gaagtacacc
     9301 gggtcgaccg actcgagcgg cacgaactcg gtgatctcgg cggtgtgcgt cccggtctcc
     9361 tcgagcgcct tcagctcctc gggcgagaac atcacgtact ggtccttggc gaactcgtag
     9421 cccttgacca tgtcctcgcg cggcaccacg acctcctcct tgacgcagag gtactgctgc
     9481 ttcaggcgcg agccgcacgc cttgtgcagc aggttgaacg agattgcgcg gctcgcctcg
     9541 gtggcggagt acagcttcac cgggatcgag acgagcccga acgagacgct gagcgatgcg
     9601 atggatcttg ccgccatggt gctctcccga cgaaggggcg cgtattatga acgcaaaact
     9661 tctgctaaat caagcggtcc cggtcaggac ttcgcgttct ccaggacctt ttcgagactc
     9721 tgcttccggg tcagggcctc ggcccagcgg tcgcccgtct tcaccaggcg ctcggccgcg
     9781 ttggtgatcc tgaagtcgag cgggtgcgct ttctcgagct cctcccaggt gagcggcatc
     9841 gagaccgcgg cgcccggaag cccgcgcggt gagtaggcga cgttcagcgt cttgccgcgc
     9901 acgttcatgt tgtagtccat gaagatcttg ccggtgcact tcgggacgct ccattccatg
     9961 gtgatgtcct tcgggtgctc gcgcatcagg tgccggccca ccagctccga caccgctcgc
    10021 gacgtgtcga agtcgatggt acgccggatc ggcacgaaca cgtgcagccc ggtcttc
//
LOCUS       DATDMW010000055         5233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1632747, whole genome shotgun sequence.
ACCESSION   DATDMW010000055 DATDMW010000000
VERSION     DATDMW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632747"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..466)
                     /locus_tag="VI319_02385"
     CDS             complement(53..466)
                     /locus_tag="VI319_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY6862721.1"
                     /translation="MKWLSIVTAVLAMTFAGGAFAQEKVVYHFDSGLSQATKGLRNIY
                     NHLEVDPKAKIVVVAHADGVDFLMEGAKDRLGPFDARVQDLIGRGVKFEVCKITLRNR
                     KLRKEQFILGVDYVPSGVVQITHLQQKEGYAYLKP"
     gene            533..802
                     /locus_tag="VI319_02390"
     CDS             533..802
                     /locus_tag="VI319_02390"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY6862722.1"
                     /translation="MAKHLLIDGRVQGVGFRYEMTRQANTLGVTGWVRNTRDGKVEAV
                     VDGPPDRVDAIIAWARRGPAAAQVTDVQVSENAGSYDEFEQWPTE"
     gene            complement(838..1056)
                     /locus_tag="VI319_02395"
     CDS             complement(838..1056)
                     /locus_tag="VI319_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862723.1"
                     /translation="MKILIILAIAAGIGWYAYKSIWDAPETCASDQQACMEKCRRTTT
                     ENQEAQACQAACTRERESCDREKARSVR"
     gene            complement(1053..4535)
                     /gene="dnaE"
                     /locus_tag="VI319_02400"
     CDS             complement(1053..4535)
                     /gene="dnaE"
                     /locus_tag="VI319_02400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY6862724.1"
                     /translation="MPKPAFVHLRLHSEYSVTDGIVRIDDAVARAAADGMPALAITDL
                     ANVFGMVKFYKAARAAGVKPVVGCDVWVSNESERDKPHRLLLLVQTHDGYLRLSDWLS
                     RAWLENQHRGRGELRRSWLAETGTAGVIALSGAMQGDVGMALLSDNRPAAERLAREWA
                     KLFPGRFYVELQRPGGPQAEAYVASAVQLAGALGLPVVATHPVQFLTPEEFTAHEARV
                     CIAEGYVLGDRRRPRAFTPKSYFLSQAEMGELFADLPEALANSVEIARRCNLSLQLGK
                     SKLPAFPTPGGLSLEDFLREQARKGLGARLRALYGDQLEDQRAKYEARLELELGTIIN
                     MGFAGYFLIVADFINWAKSNGVPVGPGRGSGAGSLVAYSLGITDLDPLRYDLLFERFL
                     NPERVSMPDFDIDFCERGRDRVIEYVKQKYGSQSVSQIATFGTMAARAVVRDVGRVME
                     WSYTRTDELAKLIPFQPGKRITLENAREMEPRLAEREKNEEETRELLALAGQLEGIVR
                     NVGMHAGGVLIAPGKLTDFCPLYAAQGTESTVSQFDMKDVERIGLVKFDFLGLTTLTI
                     LDWAGQSIRAMGEPGFALEKIPLDDPAAYAVFAQANTTAVFQFESRGMRDLLKRAKPD
                     RFEDIIALVALYRPGPMDLIPDFIERKQSGGRKVEYLDPRLEPILKPTYGIMVYQEQV
                     MQIAQAIGGYSLGGADLLRRAMGKKDAKEMAQQREIFLAGAEKNGMARGKANALFDLM
                     EKFAGYGFNKSHAAAYALVAYQTAYLKAHYPAAFMAANLSAIMDDTDKVHQFHEDALA
                     NGLKVLPPDVNTGEYRFTPVDAKTIRYGLGAVKGTGESAIGAILKAREAGPFADLFDF
                     CRRIDKRIVNRRVVEALVRAGAFDSLNRNEHDHRASLLASVGIALDAAEQAERDAQQV
                     SLFGEAESAQRTLSLVAAPRWDEVQRLREEKAALGFYLSGHPFSVHRAEVARFVRTNL
                     RNLAPSADGDARAQLIAGVVEGLRVLRTQQGRMVAINLSDGTAVQEVTIYNEVFDPIR
                     DLVKEDAIVVIEAKVRLVRRSSGDEGETTFMRIIAERVYDLATARTRFAREMRLVMNG
                     EASREAAAATAKLKELLAPYRNGPCPVAIAYQNGTASVQMRLGDGWKVSLDDRLLESL
                     RQWLRPENVEVLYS"
     gene            4667..5140
                     /locus_tag="VI319_02405"
     CDS             4667..5140
                     /locus_tag="VI319_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY6862725.1"
                     /translation="MSKIRLHVLAAALAAAAAPALADTIYKYERGDGSVVYSDTPVPD
                     AHLVERFSVAQATAQPPAPAASEARRPAAPAQDRVARLDAADAEVRAAQQAVDAAKTR
                     LGQGAEPLPGERAGIGGGRSRLTESYFARQQALQEELVRATARLEEAYRQRNDAR"
BASE COUNT          783 a         1857 c         1767 g          826 t
ORIGIN      
        1 cgctgcgggc cccggaaact ctgcagacgt tccagaaccg gtagctcagc cgtcagggct
       61 tgagatacgc gtagccttcc ttctgctgca ggtgcgtgat ctgcaccacg cccgagggca
      121 cgtagtcgac gccgaggatg aactgctcct tcctgagctt gcggttgcgc agcgtgatct
      181 tgcacacctc gaacttcacg ccgcgcccga tcaggtcctg aacgcgcgcg tcgaacggcc
      241 cgagccggtc cttcgcgcct tccatcagga aatcgacgcc gtcggcgtgc gcgacgacga
      301 cgatcttcgc cttcgggtcg acctcgagat ggttgtagat gttgcgcagg cccttggtcg
      361 cctgcgagag gccgctgtcg aagtgataga cgactttctc ctgcgcaaag gcgccgccgg
      421 cgaacgtcat agccaggacc gcggtcacaa tcgaaagcca cttcatgatt cgctccttca
      481 tgtcgaatct ccgaacttta cgccgacgtg gacccagtag gaaaatcgca ccatggctaa
      541 gcatcttcta atagacggcc gcgtccaggg cgtcggcttc cgctacgaga tgacgcggca
      601 ggccaatacg ctcggcgtga ccggctgggt ccgcaacacg cgcgacggca aggtcgaggc
      661 ggtcgtcgac ggtccgcccg acagggtcga cgcgatcatc gcgtgggcgc ggcgcgggcc
      721 ggcggcggcg caggtgaccg acgtgcaggt gagcgagaac gcggggagct acgacgagtt
      781 cgagcagtgg ccgacggagt aaaagcaggt ccttcgcttc gctcaggacg acatgtatta
      841 gcggaccgag cgggcttttt cgcgatcgca gctttcccgc tcgcgcgtgc acgccgcctg
      901 gcaggcctgc gcttcctggt tctcggtcgt ggtgcggcgg cacttctcca tgcacgcctg
      961 ctgatcgctc gcgcaggtct ccggcgcatc ccagatcgac ttgtacgcgt accagccgat
     1021 gcccgccgcg atggcgagga tgatgaggat tttcacgagt agagcacctc cacgttttcc
     1081 ggcctgagcc attgccgcag cgactcgagc aggcggtcgt cgaggctcac cttccagccg
     1141 tcgcccagcc gcatctgcac gctcgccgtc ccgttctgat acgcgatcgc caccgggcac
     1201 gggccgttgc ggtacggcgc gagcagctcc ttcagcttcg ccgtcgcggc cgccgcctcg
     1261 cgcgaggcct cgccgttcat cacgagccgc atctcgcgcg cgaagcgggt gcgcgccgtc
     1321 gcgagatcgt agacgcgctc ggcgatgatg cgcatgaacg tggtctcgcc ctcgtcgccg
     1381 ctcgagcggc gcaccagccg caccttcgcc tcgatcacga cgatcgcgtc ctccttgacg
     1441 agatcgcgga tcgggtcgaa gacctcgttg tagatcgtca cctcctgcac ggcggtgccg
     1501 tcggagaggt tgatcgcgac catccggccc tgctgcgtgc gcaggaccct caggccttcg
     1561 accacgcccg cgatcagctg cgcgcgcgcg tcgccgtcgg cggacggcgc caggttgcgc
     1621 aggttcgtgc gcacgaagcg cgcgacctcg gcgcggtgca ccgagaacgg atgccccgac
     1681 aggtagaagc cgagcgccgc cttctcctcg cgcagccgct gcacctcgtc ccagcgcggc
     1741 gcggcgacca gcgacagcgt gcgctgcgcg ctctcggcct cgccgaacag gctcacctgc
     1801 tgcgcgtcgc gctcggcctg ctccgcggcg tcgagggcga tcccgaccga ggcgaggagg
     1861 ctcgcgcggt gatcgtgctc gttccggttc agcgagtcga acgcgccggc gcgcaccagc
     1921 gcctcgacca cgcgccggtt cacgatgcgc ttgtcgatgc gccggcagaa atcgaagaga
     1981 tccgcgaacg ggccggcctc gcgcgccttc aggatcgcgc cgatcgccga ctcgcccgtg
     2041 cccttcacgg cgccgaggcc gtagcggatc gtcttcgcgt cgacgggcgt gaagcggtac
     2101 tcgccggtgt tcacgtcggg cggcaggacc ttcaggccgt tcgcgagcgc gtcctcgtgg
     2161 aactggtgca ccttgtcggt gtcgtccatg atcgccgaga ggttggccgc catgaacgcc
     2221 gccgggtagt gcgccttgag atacgccgtc tggtaggcga ccagcgcata ggccgcggcg
     2281 tgggacttgt tgaagccgta gccggcgaac ttctccatca ggtcgaagag cgcgttcgcc
     2341 ttgccgcgcg ccatgccgtt cttctccgcg cccgcgagga agatctcgcg ctgctgcgcc
     2401 atctctttcg cgtccttctt gcccatcgcg cgccgcagca ggtcggcgcc gccgagcgag
     2461 tagccgccga tcgcctgcgc gatctgcatc acctgctcct ggtacaccat gatgccgtag
     2521 gtcggcttga ggatcggctc gaggcgcggg tcgaggtact cgaccttcct gccgcccgac
     2581 tgcttgcgct cgatgaagtc ggggatcaga tccatgggcc ccggtctgta tagagccacg
     2641 agcgcgataa tgtcctcgaa gcggtcgggc ttcgcgcgct tcagcaggtc gcgcatgccg
     2701 cgcgactcga actggaacac cgcggtcgtg ttcgcctgcg cgaagaccgc gtacgcggcc
     2761 gggtcgtcga gcgggatctt ctcgagcgcg aagcccggct cgcccatcgc gcggatcgac
     2821 tgtcccgccc agtcgaggat cgtgagcgtg gtcaggccga gaaagtcgaa cttgaccagg
     2881 ccgatgcgct cgacgtcctt catgtcgaac tgcgacaccg tgctctcggt gccttgcgcc
     2941 gcgtacagcg ggcagaagtc ggtcagcttc cccggcgcga tcagcacgcc gcccgcgtgc
     3001 atgccgacgt tgcgcacgat gccctcgagc tggccggcga gcgcgagcag ctcgcgcgtc
     3061 tcctcctcat tcttctcgcg ctccgcgagc cgcggctcca tctcgcgcgc gttctcgagc
     3121 gtgatgcgct tgcccggctg gaaggggatc agcttggcga gctcgtcggt ccgcgtgtag
     3181 ctccactcca tgacgcgccc gacgtcgcgc accaccgcgc gcgccgccat ggtgccgaag
     3241 gtggcgatct gcgagacgga ttgcgagccg tacttctgct tcacgtactc gatcacgcgg
     3301 tcgcggccgc gctcgcagaa gtcgatgtcg aagtcgggca tcgacacgcg ctcgggattg
     3361 aggaagcgct cgaacagcag gtcgtagcgc agcgggtcga ggtcggtgat gccgagcgag
     3421 tacgcgacca gcgagcccgc gcccgagccg cgcccggggc cgaccggcac cccgttcgac
     3481 ttcgcccagt tgatgaaatc ggcgacgatc aggaagtagc cggcgaatcc catattgatg
     3541 atggttccga gctcgagctc caggcgcgcc tcgtacttgg cgcgctgatc ctccaactgg
     3601 tcgccgtaca gcgcccggag acgcgcgccg agcccctttc gcgcctgctc gcgcagaaaa
     3661 tcctcgagcg acaggccgcc cggcgtcggg aacgcgggca gcttcgactt ccccagctgc
     3721 agcgacaggt tgcagcggcg ggcgatctcg accgagttcg cgagcgcctc ggggagatcg
     3781 gcgaagagct ctcccatctc ggcctgcgac aggaagtagc tcttcggggt gaacgcgcgc
     3841 ggccgccggc gatcgccgag cacgtagccc tcggcgatgc agacgcgcgc ctcgtgcgcg
     3901 gtgaactctt ccggggtcag gaactgcacc gggtgcgtgg cgacgaccgg caggccgagc
     3961 gctcccgcga gctgcaccgc cgaggcgacg taggcctccg cctgcggacc gccgggacgc
     4021 tggagctcga cgtagaaccg gccggggaag agcttcgccc actcgcgcgc gaggcgctcg
     4081 gccgccggcc ggttgtccga gagcagcgcc atgccgacgt cgccttgcat cgctcccgac
     4141 agcgcgatga cgcccgccgt gccggtctcg gcgagccagc tccggcgcag ctcgccccgg
     4201 ccccggtgct gattctcgag ccaggcgcgc gacagccagt ccgagagccg caggtagccg
     4261 tcgtgcgtct ggaccagcag gagcaggcga tgcggcttgt cgcgctcgga ctcgttcgag
     4321 acccagacgt cgcagccgac gaccggcttc acgcccgccg cgcgcgccgc cttgtagaac
     4381 ttgaccatgc cgaagacgtt ggcgaggtcg gtgatggcga gggccggcat gccgtcggcg
     4441 gccgcgcggg ccaccgcgtc gtcgatgcgc acgatgccgt ccgtgaccga gtattcgctg
     4501 tgcagccgga ggtggacgaa ggcgggtttt ggcatcgtgt tcgcgccttg acggcgacga
     4561 aattctatca cccgcgcatc ggcgccggac ctccgcccgg cccataaggc acgtcgaaaa
     4621 ctcttcgcgg cgcgcgtgtt ccaatgggcc gggaaatccg tcgcttatga gcaagatccg
     4681 cctccacgtc ctcgccgccg ccctggccgc agcggccgcg cccgcgctcg ccgacaccat
     4741 ctacaagtac gagcgcggcg acggcagcgt cgtgtactcg gacaccccgg tgccggacgc
     4801 ccacctggtc gagcgcttca gcgtcgcgca ggcgaccgcg cagccgcccg cccccgccgc
     4861 gtccgaggcg cggcgcccgg ccgctccggc gcaggatcga gtcgcccggc tggatgcggc
     4921 cgacgccgag gtgcgcgccg cgcagcaggc ggtggacgcc gcgaagacgc gcctgggcca
     4981 gggcgccgag ccgcttcccg gcgagcgcgc cggcatcggc ggcggccgca gccgcctgac
     5041 cgagagctat ttcgcgcgcc agcaggcgct ccaggaggag ctggtccgcg ccaccgcgcg
     5101 gctcgaggag gcctaccgcc agcgcaacga cgcgcggtag ccgtccctat aatccccgcc
     5161 gatgagcacc gcggcgggcg ccaaatccac cgaaacgctc gctttcgcgg ccctcgtcgc
     5221 gggcgccgcc atg
//
LOCUS       DATDMW010000056         3763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_387861, whole genome shotgun sequence.
ACCESSION   DATDMW010000056 DATDMW010000000
VERSION     DATDMW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387861"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VI319_02410"
     CDS             complement(<1..582)
                     /locus_tag="VI319_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEY6862726.1"
                     /translation="MVGRVRAALEAAVGGKVSGERSVSGGDINSAHAISFTNGRRLFV
                     KTNAREPAGLFQAEAEGLAWLAEARALRVPDVVACGPGFLALELIEAAPRARDFDERL
                     GRGLAALHRFGAPRFGLDHDNFIGRLPQDNARVTEPDWPAFYRARRLEPQLRLATDEG
                     LASARMRSGFERLFARLPELCGPPEPPARLHGDL"
     gene            complement(595..1098)
                     /locus_tag="VI319_02415"
     CDS             complement(595..1098)
                     /locus_tag="VI319_02415"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY6862727.1"
                     /translation="MAAGTNQAPVHVCFVCLGNICRSPTAEAVMRHIVKQEGLEARVR
                     ISSAGTGDWHVGAPRDKRSSAVGAARGIPLSGRAQQFTTGSFEGCDYVLAMDRANRDE
                     LLRLARTDADRAKVVLLRSFDPAAPRDAEVPDPYYGGPSGFEEVFDLCEAACRAFLDH
                     LRTQHRL"
     gene            complement(1103..2899)
                     /locus_tag="VI319_02420"
     CDS             complement(1103..2899)
                     /locus_tag="VI319_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY6862728.1"
                     /translation="MPHSLLVINADGPETRVALVEDGFLGELYIERKRERGIAGNIYK
                     GRVERVLPGMQAAFVNIGLEKSAYLHVSDVRGTPDDLKGLLSGESNRNEDREDEEEER
                     DKQRAGGGARIEDLLKEGQEVIVQVTKEPISTKGARTTRYISLPGRHLVFMPTVDHIG
                     ISRRIASDKERRRLREIVESVRPPGSGFIVRTVAEGVSERELKSDMEFLIKLWNNVVQ
                     RAEGGRCPSLIYNDLDLLLRTVRDLFTTDVEKLIIDSRSEYERVKKFIAAFMPDFQGH
                     IELYEGQEPIFDGFGIEIEIDRALERKVWLKSGGYLIVDEMEALTAIDVNTGRFVGKS
                     SLEDTITKTNMEAAREVAEQLRIRSIGGMIVVDFIDMDRPHNRDKVTRAFNDYLKKDR
                     AKAAVTRISELGLIEMTRKRTRESLLHNLTEPCSACEGKGYTKSRRTVCYELFRELRR
                     QGDLVEGDTILIEVHPEVANVLATTDRPFLDDLEKRLQKRLVVKARGSFHVEDFEIRS
                     PNDKTPIEKSAAAGGGGDDKEKEPKRRRRKKRIGPPAEVEAMLAEEEAASVVDPESLA
                     DEEAQSAAQPPNGAAPQQPAGASAHSDDAAKS"
     gene            complement(3557..>3763)
                     /locus_tag="VI319_02425"
     CDS             complement(3557..>3763)
                     /locus_tag="VI319_02425"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY6862729.1"
                     /translation="ITVADCVLIGGPAAVLGPIGDLVESMIKRSVGAKDSGKLLPGHG
                     GMLDRIDALLFVSAWTYVYVLHLR"
BASE COUNT          566 a         1324 c         1228 g          645 t
ORIGIN      
        1 caagtcgccg tgcagccgcg cgggcggctc gggtggccca cagagctcgg gcaagcgcgc
       61 gaacagccgc tcgaagccgc tgcgcatgcg cgccgaggcc agcccctcgt cggtggccag
      121 ccgcagctgc ggctcgagcc ggcgcgcccg gtagaaggcg ggccaatcgg gctccgtcac
      181 gcgcgcgttg tcctgcggca accgcccgat gaagttgtca tggtcgagac cgaagcgcgg
      241 cgcgccgaac cgatgcagcg ccgcgaggcc gcgcccgagc cgttcgtcga agtcacgcgc
      301 gcgcggcgca gcctctatga gctccagcgc gaggaagcca ggcccgcacg cgacgacgtc
      361 tggcacgcgc agcgctcgcg cctcggcgag ccacgcgagc ccctcggcct cggcctggaa
      421 caggccggcc ggctcgcgcg cgttcgtctt cacaaagagc ctccgcccgt tcgtgaacga
      481 gatcgcatgg gcgctgttga tgtcgccgcc gctcacggag cgctcgcccg agacctttcc
      541 acccaccgcg gcctccagcg ccgcccgcac ccgccccacc atgcccgccc gccatcagag
      601 ccggtgctgc gtccgcaggt ggtcgaggaa cgcgcggcac gccgcctcgc acagatcgaa
      661 cacctcctcg aatccgctcg ggcctccgta gtacggatcg ggcacctcgg cgtcgcgcgg
      721 cgccgccgga tcgaacgagc gcagcaagac caccttggcg cgatcggcgt cggtgcgcgc
      781 caggcgcagg agctcgtcgc ggttcgcgcg atccatcgcc agcacgtagt cgcagccctc
      841 gaatgaaccc gtcgtgaact gctgggcacg ccccgacagc gggatgccgc gcgcggcgcc
      901 cacggcggag ctgcgcttgt cgcgcggcgc gccgacgtgc caatcacccg tgccggcgct
      961 cgagatgcgc acgcgcgcct cgaggccctc ctgcttcacg atgtgacgca tcaccgcctc
     1021 ggccgtcggc gagcggcaga tgttacccag gcagacgaag cagacgtgaa ccggagcttg
     1081 gttcgttcca gcggccacgg cgctacgact tggccgcgtc gtccgagtgc gccgaggcgc
     1141 cagccggctg ctgcggggcc gcgccgttcg gcggctgtgc ggcgctctgc gcctcttcat
     1201 cggccagcga ctcgggatcg acgacgctgg ccgcctcttc ctcggccagc atggcctcga
     1261 cctcggcagg cggaccgatg cgcttcttgc gccgccgccg cttgggctcc ttctccttgt
     1321 cgtcgccgcc gccgcccgcc gccgcggact tctcgatggg cgtcttgtcg ttgggcgagc
     1381 ggatctcgaa gtcctcgacg tggaacgaac cgcgcgcctt caccaccagg cgcttctgca
     1441 ggcgcttctc gagatcgtcc aggaacgggc ggtcggtggt ggccagcacg ttcgcgacct
     1501 cggggtgcac ctcgatgagg atcgtgtcgc cctcgaccag gtcgccctgt cgacgcagct
     1561 cgcggaagag ctcgtagcaa acggtgcggc gcgacttcgt gtagcccttg ccttcgcacg
     1621 ccgagcacgg ctcggtgagg ttgtgcagca gcgactcgcg cgtgcgcttg cgcgtcatct
     1681 cgatgagccc cagctccgag atgcgcgtca cggccgcctt cgcgcggtcc ttcttcaggt
     1741 agtcgttgaa cgcccgcgtg accttgtcgc ggttgtgcgg gcggtccatg tcgatgaagt
     1801 cgacgacgat catgccgccg atcgagcgga tgcggagctg ctccgccacc tcgcgagccg
     1861 cctccatgtt cgtcttggtg atcgtatctt cgaggctgga cttgccgacg aagcgtcccg
     1921 tgttgacgtc gatggcggtc agcgcctcca tctcgtcgac gatgaggtag ccgcccgact
     1981 tcagccacac cttgcgctcc agcgcgcggt cgatttcgat ctcgatgccg aagccgtcga
     2041 agatcggctc ctggccttcg tagagctcga tgtgcccctg gaaatcgggc atgaacgcgg
     2101 cgatgaactt cttgacgcgc tcgtactcgc tgcgcgaatc gatgatcagc ttctcgacgt
     2161 cggtcgtgaa gagatcgcgc accgtgcgca gcaagagatc gaggtcgttg tagatcagcg
     2221 agggacagcg gccgccctcg gcgcgctgga cgacgttgtt ccaaagtttg atcaggaact
     2281 ccatgtccga cttcagctcg cgctcggaca cgccctcggc gaccgtgcgg acgatgaagc
     2341 cggagcccgg cgggcgcacc gactcgacga tctctcgcag ccgacgacgc tccttgtcgg
     2401 aagcgatgcg gcgcgagatg ccgatgtggt cgaccgtcgg catgaacacg aggtggcgcc
     2461 cgggcagcga gatgtagcgc gtcgtccgcg cccccttcgt gctgatgggc tccttcgtga
     2521 cctggacgat gacctcctgg ccttctttga ggaggtcttc gatgcgggca ccgccgccgg
     2581 cgcgttgctt gtcgcgctcc tcttcttcgt cctcccgatc ctcgttccga ttggactcgc
     2641 ccgacagcaa ccctttcagg tcgtccggcg tgccccggac gtcggagacg tgcaggtagg
     2701 cgctcttttc gaggccgatg ttgacgaaag cggcctgcat cccagggagc acgcgctcga
     2761 cgcggccctt gtagatgttt ccggcgatac ctcgctcgcg cttgcgttcg atgtagagct
     2821 caccgaggaa cccatcttcg acgagcgcga cgcgggtctc cgggccgtcg gcgttgataa
     2881 ccaggaggct atgcggcatg gacacgcttc acggaggcta cgaaaattta cggggagcga
     2941 cggtcccgcc tgacgggaat gtcagggtcc gtcgcgaaaa tgttgtgaga tcgatcgcgg
     3001 tcgtaacacg gaaccgcccg caattcaacg acatcggctt gatcggctca ggtcggcaca
     3061 tcccttgctc cccagaagcg gcaagaacga cgagtcggcg cttcctcctc ctcctcctaa
     3121 cttaaagcgc ccgcccgctg ttctcctcct cccttcacac cgacttcggt cggtgtgacc
     3181 tagattgacg gcctcccgaa taaatctgtc atgcgtggcg gccgtgccca tatgaaagtt
     3241 tttccttagc ctgactttgc ctcgcctctc acacgcttaa ggcctagtta gcttcgcagt
     3301 tagtttggca accctcgcgg gagcacctcc cccgcggggg tttttgccgt ttcgatctga
     3361 cggggtacgg ctcgctgccg ctcgcgcacc ccggtccccc tcgctccgcg cggcaaagcc
     3421 gcgctgcgct cgcaccagga aggcggacaa tgctaacgcg ggtgtagcgt ctgccgcagg
     3481 tgcagcgcct gccgcaggaa gggcgcttac cggtgcagcg ccaccgcagg tgcagcgcct
     3541 gccgcaggtg gggcgcttac cgcaggtgca ggacgtagac gtaggtccag gcggagacga
     3601 acagcagggc gtcgatgcgg tccagcatgc cgccgtgccc cggcaggagc ttgcccgagt
     3661 ccttggcgcc caccgagcgc ttgatcatcg actcgaccag gtcgccgatc gggcccagga
     3721 cggccgccgg ccctccgatg agcacgcagt ccgcgaccgt gat
//
LOCUS       DATDMW010000057        12256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_885506, whole genome shotgun sequence.
ACCESSION   DATDMW010000057 DATDMW010000000
VERSION     DATDMW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12256
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885506"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VI319_02430"
     CDS             <1..783
                     /locus_tag="VI319_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY6862730.1"
                     /translation="GVDTPRVGERVVTLQFVKQASPTSFPVVLGLHVWGGYAEYVKVP
                     ATVTHPIPDGVDFASACVVSRHAPVAISMLRDGARLKPGEWVLVMGAAGGLGSAGVQA
                     AKVLGAKVIAAAGAPERVRAAVALGADAGVDYRAQDLTGEVMRITGGRGADVVFENIG
                     DAVLFPKAFAAIARNGRLITAGAHGGGTVPLDVKKLYLNAITVIGSFGRIIREDVALA
                     LSAAADGRYKALIDRVLPLELAAEAHRLVAERSGIGKIVLQP"
     gene            806..1762
                     /locus_tag="VI319_02435"
     CDS             806..1762
                     /locus_tag="VI319_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862731.1"
                     /translation="MIRMLVTASIAAAVASPCAAQVPDRPVTMLAGFPAGGLVDIVAR
                     VVAEGMKPHFPKGIVVVNKPGAGGAVAVAETVQARPDGTTILLTPQSSLVIPPQQTAQ
                     LAYKTPDDYDPVINVISYYPLLVVKSDAPWKTIQAFVADARSNPGKMRIGTPGEGTAS
                     HLNLEEFIFRTETRMTPVPYAGWAQSSVALLGGHIEAVVAQPGEAKPQVDGGKMRALA
                     VFQGARHPAFPGAPTAKEAGWDVGIGVWYLIVAPKGTPAAALKQIHDAGKAAIEQKSF
                     ADTMHARGVDVDYRAGEALRADLWREYKELGAVMRRIGIVKQ"
     gene            complement(1766..2932)
                     /locus_tag="VI319_02440"
     CDS             complement(1766..2932)
                     /locus_tag="VI319_02440"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEY6862732.1"
                     /translation="MLRIARKLLLAVLLLAAALPAGAQEQRTFSQAELDQMLAPVALY
                     PDPLLSQMLMAATYPLEVVEAARWSKANPGLQGDAAVNAAAGQGWDPSVTSLVAFPQV
                     LAWMDENLQWTRAVGDAFLAQQPQVMDTVQGLRARAQAAGNLPSDDRVVVTQQGPTIV
                     VVPADPQVVYVPYYDTTVVYGTWWWPAYPPVVWRPWRGYVARPRAAVAWTPGVRVTAG
                     FFFGAFDWRRREARVVRVNNYYYNRTVTVNRAPGSWQHDPAHRRGAGYRVPAAAPRAY
                     APPPAVREERRGLLGTEPPREERRIEERRDERREERRNRRPEEQREQRPGVFPPGQGG
                     FSPGPGGFSPGPGGFSPGPGGIQQPRREPGARLEGTREHQAERERRRHGEHHPA"
     gene            3054..3959
                     /locus_tag="VI319_02445"
     CDS             3054..3959
                     /locus_tag="VI319_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6862733.1"
                     /translation="MIRNLQHIALAVPDPAAGKRFYTDFGMEGRDVGSSIVMRCAGRD
                     QDQVVLVEGRKKQQHHVCFGTRAAELDALKKRLEKEGVKLVDAPKETPGDGIWFRDPD
                     GLLVNVRVAEAAPWRKAPEWKLNTPGCLNRSGTCGHPKRGMKVQPTRLGHTLRFTPQM
                     DKMLDFYLRVVGLQLSDRAQDIVAFMRVHGNSDHHVFGFIKSDRPGFHHASFEVENID
                     AIGMGACNLLDKGYKDGWGLGRHVIGSNFFHYIRDPWNSLAEYFCDIDYIPEGYDWKA
                     TNYAPEDALYVWGPRPPEAFGQNFE"
     gene            3982..4947
                     /locus_tag="VI319_02450"
     CDS             3982..4947
                     /locus_tag="VI319_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862734.1"
                     /translation="MRSIFALACALLAAAWTPVHAQQGWPDKPVRLVVSFAPGGVHDT
                     LARVLQPKLTEALGQPIVIENRAGAGGNIAAEAVAKSAPDGYTFLIASEAIATNEYLY
                     RKLAYDPYKDLAPVAKLADYPMALIVHPSLWVDNVRDLVALAQAKPGTISYGSAGVGA
                     SGHLAGELFKSVTDVDMVHVPYKGGAPALADLIAGRIQFMFLSVSLTVPQVKQGKVKV
                     IGVTGPRRSPELPDVPTTTEQGFPQVQALLFSSMLAPAGTPPAIVKRMNEVVVKALGS
                     PDVQKRLADLGAVPAPTSPEGFRKVLHDEGERWGKLIREKNIRAD"
     gene            4975..6534
                     /locus_tag="VI319_02455"
     CDS             4975..6534
                     /locus_tag="VI319_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEY6862735.1"
                     /translation="MELLVTILFLLLCSVVLGWIARHFAFPYPIALVIGGAALGFAPH
                     LPQFPFEPESILILFLPPILYQAALLTSWRDFRANLRPISMLAIGLVIVTTLAVGAAA
                     KWLIPDLPWAAAFVLGAIVSPPDAVAATAILSRLRIPRRVVTVLEGESLVNDASGLVM
                     YKFAVAAVLTGGFSLAGASVEFVGVAVGGVAVGVTLGLLFVFIHRHLHDAFIEVLTSL
                     SVPYVAYIVAEAVHVSGVLAVVAAGLVRGRYSPEIVSAEMRILARSVWNILVFLLNSL
                     IFILIGMQLSAILSRLTGYSGRDLFLYGMLVSMVAIAVRFLWIYPATYLPRVFSARVR
                     ERGPAPPEAETFIMSWCGMRGIVSLAAALALPEELASGAPFPKRDLIIFLTFVVIAVT
                     LVLQGLSMRPLIRRLQVGTDWSTQEEEQLARLAMGKAALAAIDGMAQSENVAHDVAER
                     IRAEFAEQVTVAVGPGVVLSPGSDLVKRLRRTAVRAERQELIRIWRANQISDEVLHRM
                     EESLDYQESRF"
     gene            6578..7648
                     /locus_tag="VI319_02460"
     CDS             6578..7648
                     /locus_tag="VI319_02460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY6862736.1"
                     /translation="MAQGASFPESPLEFGVFDWIEWRDAPPAEIFEHKLAIAERADAA
                     GFHGWHIAEHQGTPLSIDGSPSLVLSAAIQRTKRLRLGALTWCLPWYNPFRWYSEICM
                     LDHMSGGRIELGVGRGVSPLESSYFGMKSVDESRERYRETLDIFFRACANPKLDFEGK
                     YFSYKDLELYIHPKQRPYPPLWFPSSDRNSIDFTARHGYHTVLNTSNEEAGALYAQYR
                     ETWVAHRDDPGRHNAHVAAPKLGKSQHVFVADTEKEAAEIGMAAYKTWASHLTHLTRR
                     HGKSNLLNVDPTSPDAPLRFVGGTPGSVAQQLDEVVRRAGINYLLLVFSFGDLAPERA
                     LHSMDLFVREVMPALSTRRRRP"
     gene            7645..8826
                     /locus_tag="VI319_02465"
     CDS             7645..8826
                     /locus_tag="VI319_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862737.1"
                     /translation="MKKAVWLAAAAALLALPAAAQTIKIGFITSYSGLNGNLGPYMER
                     AVRLYMKLHGKELPPGVKIELLVRDDTGPNPDKAKQLAQELIVRDKVNLLAGVIFTPN
                     ALAIAPLATEAKVPFVIMNAGTSVITTRSPYIVRVSFTLWQSCYPLGQWAAKRFKTSY
                     TMVSDFGPGHDCEDAFARAAKENGGQVVGSVRVPLQNPDFVPFIQRVKDAKPEALMVF
                     VPAGKTATAVLKAFSELGLKSAGVKLIGPGDVTPDEELQGMGDVALGTFTVHHYSADA
                     ERPANKAFVAAWKKEYGASEVPDFLSAASWDGMDMIFQAIKQQKGRLDPDKTLEIFKG
                     YKSASSPRGPISIDPETRDIVQNEYLREVRKVGGRLANVELETVGTAVKDPWKELQKK
                     K"
     gene            complement(8834..9121)
                     /locus_tag="VI319_02470"
     CDS             complement(8834..9121)
                     /locus_tag="VI319_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862738.1"
                     /translation="MAHPLPPQQPDPSGANDESMNAADGVLEPVESLLDLLNELNQHL
                     SHLEYALLDLKEALVQVDGGERRKLQEEAASQLERIRAAAKNDPGFPRLSE"
     gene            complement(9222..10406)
                     /locus_tag="VI319_02475"
     CDS             complement(9222..10406)
                     /locus_tag="VI319_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6862739.1"
                     /translation="MTIGGAGMYSMAVALPPLQAEFGIARSDASLPYTLTMIGFGLGG
                     IFMGRLSDRFGVVVPVLIGAICLGLGFVAAGSARAMWQFNLAQGALIGFLGTSGSFVP
                     LVADISLWFVRRRGIAIAIVMCGNYLAGTVWPPLMQHAIDAAGWRATFVGVGVFCLLT
                     MPMIALALRRRPPAIAGAAASSSGSRAAHEAADRSRPLGLAPASLQSLLCVAGVACCV
                     AMSMPQVHMVAYCGDLGYPAARGAEMLSLMLGFGIASRLVSGWIADRIGGLRTLLLGA
                     VLQAVALALFLPFTQLGALYVVSALFGLFQGGLVPSYPLIVREHFAPREAGSRTGTVL
                     MATLFGMALGGWLPGWIFDVTGSYRAAFVNGIAWNLVTTSIAGFLILRERRLGRAATA
                     HA"
     gene            complement(10491..11138)
                     /locus_tag="VI319_02480"
     CDS             complement(10491..11138)
                     /locus_tag="VI319_02480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6862740.1"
                     /translation="MRSAVLAWLLASALAVPIAASAQSPTTHEHRFTDADKWAHVFDD
                     PSRDAWQKPHEVIRALAPAPDAKIADIGSGTGYFSVRLAHAVPKGRVYGVDTEPDMVK
                     YLADRARRERLANVVAVAGRPDDAALPEKVDLVLMVDVYHHIADREAYFGRLRGSLKP
                     GARVAIIDFSETSKDGPPRHERISPTRVRAEMQRAGYTLADEHAFLPNQYFLVFR"
     gene            complement(11149..>12256)
                     /locus_tag="VI319_02485"
     CDS             complement(11149..>12256)
                     /locus_tag="VI319_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6862741.1"
                     /translation="SAAEAANRSKDEFLAMLGHELRNPLGAVSNAAQVLDREGAKPEQ
                     IAFARGVIARQTQHLARLIDDLLDVGRVITGKIYLQRERMDLSAAVRGTLDAFHASGR
                     FAGHRPTFDLGPVWIDGDRTRMEQVVTNLVSNALAYTPAGGSIRVSARREGGDAVLEV
                     ADDGIGMTPDELKRVFDLFYQGKSELHRKGGLGLGLTLVHRLVELHGGIVTARSDGPG
                     RGATFTVRLPAVEAPAADARPPAPPAALRPLQVLIVEDNADARVSLQIVLESEGHTVH
                     TAADGKAGLAAFERIVPDVALIDIGLPGMDGYELARAIRARHAGPTLVALTGYGQPED
                     TQRAAQAGFDAHLVKPADIAKLRALLARRRRSDA"
BASE COUNT         1844 a         4310 c         4359 g         1743 t
ORIGIN      
        1 ggcgtcgaca cgccgcgcgt cggcgagcgc gtcgtcaccc tgcagttcgt gaagcaggcg
       61 agcccgacct cgttcccggt cgtgctcggg ctgcacgtgt ggggcggcta cgccgagtac
      121 gtgaaggtgc cggcgacggt cacgcacccg atccccgacg gcgtcgactt cgcctcggcg
      181 tgcgtggtct cgcgccatgc gccggtcgcg atctcgatgc tgcgcgacgg cgcgcggctc
      241 aagcccggcg agtgggtgct cgtcatgggc gccgcgggcg ggctgggcag cgccggcgtc
      301 caggcggcga aggtgctcgg cgcgaaagtg atcgcggccg cgggcgcgcc cgagcgcgtg
      361 cgcgccgcgg tcgcgctcgg cgccgacgcg ggcgtcgact accgcgcgca ggacctgacc
      421 ggagaggtga tgcgcatcac gggggggcgc ggcgccgacg tcgtcttcga gaacatcggc
      481 gacgccgtgc tcttccccaa agccttcgcc gcgatcgccc gcaacggccg cctgatcacg
      541 gccggcgcgc acggcggagg caccgtgccg ctcgacgtga agaagctcta cctgaacgcc
      601 atcaccgtga tcggctcgtt cgggcgcatc atccgcgagg acgtcgccct cgcgctgagc
      661 gcggccgccg acgggcgcta caaggcgctc atcgaccgcg tgctgccgct cgagctcgcc
      721 gccgaggcgc accggctggt cgccgagcgc tcgggcatcg gcaagatcgt ccttcagccc
      781 tgaatcgaca attcgaggag gcctcatgat ccgcatgctc gtcaccgcat cgatcgccgc
      841 cgccgtcgcg tcgccctgcg ccgcgcaggt tcccgaccgc ccggtcacca tgctcgccgg
      901 ctttcccgcc ggcggactgg tcgacatcgt cgcgcgcgtg gtcgccgagg ggatgaagcc
      961 gcacttcccc aaaggcatcg tcgtcgtcaa caagccgggc gccggcggcg cggtcgcggt
     1021 cgcggagacg gtgcaggcca ggcccgacgg caccaccatc ctgctcacgc cgcagtcgtc
     1081 gctcgtcatc ccgccgcagc agaccgcgca gctcgcgtac aagacgcccg acgattacga
     1141 cccggtgatc aacgtcatct cgtattaccc gctgctcgtg gtgaagagcg acgcgccgtg
     1201 gaagacgatc caggccttcg tcgccgacgc caggtccaac cccggcaaga tgcgcatcgg
     1261 cacgccgggc gaaggcaccg cgagccacct caacctcgag gagttcatct tccgcacgga
     1321 gaccaggatg acgccggtgc cgtacgcggg ctgggcgcag tcgtcggtcg cgctcctcgg
     1381 cggacacatc gaggcggtgg tcgcgcagcc cggcgaggcg aagccgcagg tcgacggcgg
     1441 caagatgcgc gcgctcgcgg tgttccaggg cgcgcgccat cccgcgttcc ccggcgcgcc
     1501 gaccgcgaag gaggcgggct gggacgtggg catcggcgtg tggtacctga tcgtcgcgcc
     1561 gaaggggacg cccgccgcgg cgctgaagca gatccacgac gcgggcaagg ccgcgatcga
     1621 gcagaagtcc ttcgccgaca cgatgcacgc gcgcggcgtc gacgtggact accgcgcggg
     1681 cgaggcgctg cgcgccgacc tgtggaggga gtacaaggag ctcggcgcgg tgatgcggcg
     1741 catcggcatc gtcaagcaat agcgctcagg ccgggtggtg ctcgccgtgc cggcggcgct
     1801 cgcgctcggc ctggtgctcg cgcgtgccct cgagtcgggc gcccggctcg cggcgcggct
     1861 gctggatccc gccgggaccc ggggagaatc ctccgggtcc gggcgaaaac cccccgggcc
     1921 cgggtgagaa gccgccctgc cccggcggga agacgcccgg acgctgctcg cgctgctctt
     1981 cggggcggcg gttccggcgc tcctcgcgac gctcgtcccg gcgctcctcg atgcgccgtt
     2041 cctcgcgcgg aggctcggtg ccgagcaggc cgcggcgttc ctcgcgcacc gcaggcggcg
     2101 gggcataggc gcgcggtgcc gcggcgggca cgcggtaacc ggcgccgcgg cgatgcgcgg
     2161 gatcgtgctg ccacgagccg ggggcccggt tcacggtcac cgtgcggttg tagtagtaat
     2221 tgttgacgcg cacgacgcgc gcctcgcggc gccgccagtc gaaggcgccg aagaagaacc
     2281 cggccgtgac gcgcacgccc ggcgtccatg ccaccgccgc gcgcgggcgc gcgacgtagc
     2341 cgcgccacgg gcgccacacc accggcggat aggcgggcca ccaccacgtg ccgtacacga
     2401 cggtcgtgtc gtagtacggc acgtacacga cctgcggatc ggcggggacc acgacgatcg
     2461 tggggccttg ctgcgtgacg acgacgcggt cgtccgaggg caggttgccc gcggcctggg
     2521 cccgcgcgcg caggccctgc accgtgtcca tgacctgcgg ctgctgcgcg aggaacgcgt
     2581 cgcccaccgc gcgcgtccac tgcaggttct cgtccatcca ggcgagcacc tgcggaaaag
     2641 cgacgagcga ggtgacgctc ggatcccagc cctggccggc ggcggcattc accgcggcgt
     2701 cgccctgcag ccccgggttg gccttcgacc agcgcgccgc ctcgaccacc tcgagcggat
     2761 aagtcgccgc catcagcatc tgcgacagca gcggatcggg gtagagcgcg accggcgcga
     2821 gcatctggtc gagctcggcc tgcgagaagg tgcgctgctc ctgcgcgccg gccggcagcg
     2881 cggccgcgag cagcagcacg gcgagcagca gcttcctggc gatacgcagc atggcgcctc
     2941 ctgagagccc ttattgtctc ttcgaacgcg ggtcctgtcc cgccggttca cgtaagaagt
     3001 gcaaccgggt gtaacgaatc tataatgggt ctcgaacgcc gcaggaggag ctcatgatcc
     3061 gcaatcttca gcacatcgcg ctcgccgtgc ccgacccggc cgccggcaag cggttctaca
     3121 ccgatttcgg catggaaggc cgcgacgtcg gcagcagcat cgtcatgcgc tgcgcggggc
     3181 gcgaccagga ccaggtcgtg ctcgtcgaag gccgcaagaa gcagcagcac cacgtctgct
     3241 tcggcacgcg cgcggccgag ctcgacgcgc tgaaaaagag gctcgagaag gaaggcgtga
     3301 agctcgtgga cgcgccgaag gagacgcccg gggacggcat ctggttccgc gaccccgacg
     3361 gcctgctcgt caacgtgcgc gtcgcggagg ccgcgccgtg gcgcaaggcg cccgagtgga
     3421 agctgaacac cccgggctgc ctgaaccgct cgggcacctg cggccatccc aagcgcggca
     3481 tgaaggtgca gccgacgcgc ctcggccata cgctgcgctt cacgccgcag atggacaaga
     3541 tgctcgactt ctacctgcgc gtcgtcggcc tgcagctctc cgaccgcgcg caggacatcg
     3601 tcgccttcat gcgcgtgcac ggcaacagcg accaccacgt gttcggcttc atcaagtccg
     3661 accgcccggg ctttcaccac gcgagcttcg aggtcgagaa catcgacgcg atcggcatgg
     3721 gcgcgtgcaa tctgctcgac aagggctaca aggacggctg gggcctgggc cggcacgtga
     3781 tcggctcgaa cttcttccac tacatccgcg acccgtggaa cagcctcgcg gagtacttct
     3841 gcgacatcga ctacatcccc gagggctacg actggaaggc gaccaattac gctcccgagg
     3901 acgcgctcta cgtctggggc ccgaggccgc ccgaggcgtt cggccagaat ttcgaatagc
     3961 aaaacggacg gaggaaggac gatgaggagc atcttcgcgc tcgcgtgcgc gctgctggcg
     4021 gccgcgtgga cgccggtgca cgcgcaacag ggctggccgg acaagccggt caggctcgtg
     4081 gtctcgttcg cgcccggcgg cgtgcacgac acgctcgcgc gcgtgctcca gcccaagctc
     4141 accgaggcgc tcgggcagcc gatcgtgatc gagaaccgcg cgggcgcggg cggcaacatc
     4201 gccgccgagg cggtcgcgaa gtcggctccc gacggctaca ccttcctcat cgcgagcgag
     4261 gcgatcgcga ccaacgagta tctctaccgc aagctcgcct acgacccgta caaggacctg
     4321 gcgccggtgg cgaagctcgc cgactatccg atggcgctga tcgtgcaccc gtcgctctgg
     4381 gtcgacaacg tgcgcgacct ggtcgcgctc gcgcaggcca agcccgggac gatcagctac
     4441 ggctcggccg gcgtcggcgc ctcggggcac ctcgcgggcg agctgttcaa gagcgtgacc
     4501 gatgtcgaca tggtgcacgt cccgtacaag ggcggcgcgc ccgcgctcgc ggacctgatc
     4561 gcgggccgca tccagttcat gttcctgtcg gtgtcgctca ccgtgccgca ggtgaagcag
     4621 ggcaaggtca aggtcatcgg cgtcaccggg ccgcggcgct cgcccgagct gcccgacgtg
     4681 ccgaccacga ccgagcaggg cttcccgcag gtgcaggcgc tgctgttctc gagcatgctc
     4741 gcgccggcgg gcacgccgcc cgcgatcgtg aagcgcatga acgaggtggt ggtgaaggcg
     4801 ctcgggtcgc ccgacgtgca gaagcggctg gccgacctcg gcgcggtgcc ggcgccgacg
     4861 tcgcccgaag gcttcaggaa ggtcctgcac gacgagggcg agcgctgggg caagctcatc
     4921 cgcgagaaga acatccgcgc cgattagcgt gcgcgcgcgc gttccgggga ccgcgtggag
     4981 ctgctggtaa cgatcctgtt cctgctgctg tgctcggtcg tcctcggctg gatcgcgcgc
     5041 cacttcgcct ttccctatcc catcgcgctc gtgatcggcg gcgccgcgct cggcttcgcg
     5101 ccgcacctgc cgcagttccc gttcgagccc gagtcgatcc tcatcctgtt cctgccgccg
     5161 atcctgtacc aggcggcgct gctcacgtcg tggcgcgact tccgcgccaa cctgcgcccg
     5221 atcagcatgc tcgcgatcgg cctggtgatc gtgaccacgc tcgccgtcgg cgccgcggcg
     5281 aagtggctga tcccggacct gccctgggcc gccgcgttcg tgctcggcgc gatcgtgtcc
     5341 ccgcccgacg cggtcgcggc gaccgcgatc ctctcgcgcc tgcgcatccc gcgccgcgtg
     5401 gtgaccgtgc tcgagggcga gagcctggtg aacgacgcct cgggcctggt gatgtacaag
     5461 ttcgcggtcg ccgccgtgct aaccggggga ttctcgctcg cgggggcgag cgtggagttc
     5521 gtcggcgtgg cggtcggcgg cgtcgcggtc ggcgtgacgc tcggcctgct cttcgttttc
     5581 atccaccggc acctgcacga cgcgttcatc gaggtgctca cctcgctctc ggtgccctac
     5641 gtcgcctaca tcgtggccga ggcggtgcac gtctcgggcg tgctggccgt ggtcgccgcc
     5701 ggcctggtgc gcggccgcta ctcccccgag atcgtgtccg ccgagatgcg catcctcgcg
     5761 aggtccgtct ggaacatcct cgtgttcctg ctgaacagcc tgatcttcat cctgatcggc
     5821 atgcagctct ccgcgatcct ctcgcggctc accggctact ccgggcgcga cctgttcctc
     5881 tacgggatgc tggtgagcat ggtggcgatc gcggtgcgct tcctgtggat ctatccggcg
     5941 acgtacctgc cgcgcgtgtt cagcgcgcgc gtgcgcgagc gcgggccggc gccgcccgag
     6001 gccgagacct tcatcatgag ctggtgcgga atgcgcggca tcgtctcgct cgccgccgcg
     6061 ctcgccctgc ccgaggagct cgcgagcggc gcgcctttcc ccaagcgcga cctcatcatc
     6121 ttcctcacct tcgtggtgat cgcggtgacg ctggtgctgc agggactgtc gatgcggccg
     6181 ctcatccgcc gtctccaggt cgggaccgac tggagcaccc aggaggagga gcagctcgcg
     6241 cggctcgcga tgggcaaggc cgcgctcgcc gcgatcgacg gcatggcgca gagcgagaac
     6301 gtggcgcacg atgtcgccga gcgcatccgc gcggagttcg ccgagcaggt gaccgtggcg
     6361 gtcggcccgg gcgtggtgtt gagccccggc tccgacctgg tgaagcggct gcggcggacc
     6421 gcggtccgcg ccgagcgcca ggagctgatc cgcatctggc gcgcgaacca gatcagcgac
     6481 gaggtgctcc accgcatgga ggagtcgctc gactaccagg agtcgcggtt ctagcgggcg
     6541 atgctagact gcgccggaat cggctgagga gcgtttggtg gcgcaaggcg catcatttcc
     6601 cgaatccccg ctcgagttcg gcgtgttcga ctggatcgaa tggcgcgatg cgccgcccgc
     6661 ggagatcttc gagcacaagc tcgccatcgc cgagcgcgcc gacgcggccg ggttccacgg
     6721 ctggcacatc gccgagcacc agggcacgcc gctctcgatc gacggctcgc cctcgctcgt
     6781 gctctcggcc gcgatccagc gcacgaagcg cctgcgcctg ggcgcgctca cctggtgcct
     6841 gccctggtac aacccgttcc gctggtacag cgagatctgc atgctcgacc acatgagcgg
     6901 cgggcgcatc gagctcggcg tcgggcgcgg cgtgtcgccg ctcgagtctt cgtacttcgg
     6961 catgaagagc gtcgacgagt cgcgggaacg gtatcgggag acgctggaca tcttcttcag
     7021 ggcctgcgca aaccccaagc tggacttcga aggaaagtat ttttcctata aggatctcga
     7081 gctctacatc cacccgaagc agcgtcccta cccgccgctg tggttcccga gcagcgaccg
     7141 caactcgatc gacttcaccg cgcgccacgg ctaccacacc gtgctcaaca ccagcaacga
     7201 ggaggcgggc gcgctctacg cgcagtaccg cgagacctgg gtcgcgcacc gcgacgaccc
     7261 gggacggcac aacgcgcatg tcgccgcgcc caagctcggc aagtcgcagc acgtgttcgt
     7321 cgccgatacc gagaaggaag cggccgagat cggcatggcg gcgtacaaga cctgggcgag
     7381 ccacctcacg cacctcacgc ggcggcacgg gaagtcgaac ctgctcaacg tcgatcccac
     7441 gtctcccgac gcgccgctgc gcttcgtcgg cgggacgccc gggtcggtgg cgcagcagct
     7501 cgacgaggtg gtgcgccgcg cgggaatcaa ttacctgctg ctggtgttct cgttcggcga
     7561 cctcgccccc gagcgtgcct tgcattcgat ggatctgttc gtgcgcgagg tgatgcccgc
     7621 gctttcaacc cggaggagga gaccatgaag aaggcggtat ggcttgccgc ggcggctgca
     7681 ttgctggcgc tgcccgcggc ggcgcagacg atcaagatcg gcttcatcac cagctactcg
     7741 ggcctcaacg gcaacctcgg cccctacatg gagcgcgcgg tgcggctgta catgaagctg
     7801 cacgggaagg agctgccccc cggcgtcaag atcgagctcc tggtccgcga cgacacgggc
     7861 ccgaaccccg acaaggcgaa gcagctcgct caggagctga tcgtgcgcga caaggtgaac
     7921 ctgctcgccg gcgtcatctt cacgccgaac gcgctcgcga tcgcgccgct cgcgaccgag
     7981 gcgaaggtgc cgttcgtgat catgaacgcg ggcacgtcgg tgatcacgac gcgctcgccc
     8041 tacatcgtgc gcgtgtcgtt cacgctgtgg cagtcgtgct atccgctcgg ccagtgggcg
     8101 gcgaagcgct tcaagacctc gtacacgatg gtgagcgact tcggtcccgg ccacgactgc
     8161 gaggacgcgt tcgcgcgcgc ggcgaaggag aacggcggcc aggtcgtcgg cagcgtgcgc
     8221 gtgccgctgc agaaccccga cttcgtgccg ttcatccagc gcgtgaagga cgcgaagccc
     8281 gaggcgctga tggtgttcgt cccggccggc aagaccgcca ccgcggtgct gaaggcgttc
     8341 agcgagctcg gcctgaagag cgcgggcgtg aagctgatcg gccccggcga cgtcacgccc
     8401 gacgaggagc tgcagggcat gggcgacgtc gcgctcggca ccttcaccgt gcaccactac
     8461 tcggccgacg ccgagcgtcc cgcgaacaag gccttcgtcg ccgcgtggaa gaaggagtac
     8521 ggcgcgagcg aggtgcccga cttcctgtcg gccgcgtcct gggacggcat ggacatgatc
     8581 ttccaggcga tcaagcagca gaagggcagg ctcgatcccg acaagaccct ggagatcttc
     8641 aagggctaca agagcgcgag cagcccgcgc gggccgatct cgatcgaccc ggagacgcgc
     8701 gacatcgtgc agaacgagta cctgcgcgag gtacgcaagg tcggcggccg gctcgcgaac
     8761 gtcgagctcg agaccgtggg gaccgcggtg aaggacccct ggaaggagct gcagaagaag
     8821 aagtagtagc ctgctactcg ctcaggcgcg ggaatcccgg gtcgttcttc gcggcggccc
     8881 ggatgcgctc gagctgcgac gccgcctcct cctgcagctt gcggcgctcg ccgccgtcca
     8941 cctgcaccag cgcttccttc aggtcgagca gcgcgtattc gaggtgactg aggtgctggt
     9001 tcagctcgtt cagcagatcg agcaggctct cgaccggctc gagcacgccg tccgcggcgt
     9061 tcatggattc gtcgtttgcg cccgacgggt cgggctgctg cgggggaagc gggtgcgcca
     9121 tgatttgttc gcctcgtttg atcgaggcca ttgtggcggc ggaccggtta caggattgcg
     9181 aggcggcccc acccctgccg tgagcactat ggggggaggc gtcaggcgtg cgcagtcgcc
     9241 gcgcggccga ggcgccgctc gcgcaggatc aggaacccgg cgatgctggt cgtgaccagg
     9301 ttccaggcga tgccgttcac gaacgcggcg cgatacgagc ccgtgacatc gaagatccat
     9361 cccgggagcc agccgccgag cgccatcccg aacagcgtcg ccatcagcac cgtgccggtg
     9421 cgcgatcccg cctcgcgcgg cgcgaagtgc tcgcgcacga tgagcggata cgacggcacg
     9481 agcccgccct ggaacagccc gaagagcgcc gacaccacgt acagcgcgcc gagctgcgtg
     9541 aacggcagga acagcgcgag cgcaaccgcc tgcagcaccg cgccgagcag cagcgtgcgc
     9601 aggccgccga tgcggtcggc gatccagccg gagacgaggc ggctggcgat gccgaagccg
     9661 agcatgagcg agagcatctc ggcgccgcgc gcggccgggt agccgaggtc gccgcagtac
     9721 gcgaccatgt gcacctgcgg catcgacatc gccacgcagc aagcgacgcc ggcgacgcac
     9781 agcagcgact gcagcgacgc gggcgcgagc ccgagcggcc gcgagcggtc ggccgcttcg
     9841 tgcgccgcgc gcgatccgga cgaagaagcg gcggcgcccg cgatcgcggg cggccgccgc
     9901 cgcaaagcga gcgcgatcat cggcatcgtc agcaggcaga agacgccgac gccgacgaag
     9961 gtcgcgcgcc agcccgcggc gtcgatcgcg tgctgcatga gcggcggcca caccgtgccc
    10021 gcgaggtagt tgccgcacat gacgatcgcg atcgcgatgc cgcgccggcg cacgaaccac
    10081 agcgagatgt cggcgaccag cggcacgaag ctgcccgacg tgccgaggaa gccgatcaac
    10141 gcgccttgcg cgaggttgaa ctgccacatc gcccttgccg acccggcggc gacgaacccg
    10201 aggccgaggc agatcgcccc gatcagcacc ggcaccacga cgccgaagcg gtccgagaga
    10261 cgccccatga agatgccgcc cagaccgaag ccgatcatcg tcagcgtgta gggcagcgac
    10321 gcgtcggagc gcgcgatccc gaactcggcc tgcagcggcg gcagcgcgac cgccatcgag
    10381 tacatgcccg cgccgccgat ggtcatcagc gcgagcgcgg cggcgagccg cgcccaggcg
    10441 taaggcgagt cgggcgcgcc gcgggggcgg aggtccggtc ccacggcggc ctagcggaag
    10501 acgaggaagt actggttcgg gaggaacgcg tgctcgtcgg cgagcgtgta gcccgcgcgc
    10561 tgcatctcgg cgcggacgcg cgtcggcgag atgcgctcgt ggcgcggcgg accgtccttc
    10621 gaggtctcgc tgaagtcgat gatcgctacg cgcgcgccgg gcttgagcga gccgcgcagc
    10681 cgcccgaaat aggcttcgcg gtcggcgata tggtggtaga cgtcgaccat cagcaccagg
    10741 tcgaccttct cgggcagcgc ggcgtcgtcg ggcctgcccg cgaccgcgac gacgttcgcg
    10801 aggcgctcgc ggcgcgcccg gtcggcgagg tacttcacca tgtcgggctc ggtgtcgacg
    10861 ccgtagacgc ggcctttcgg gaccgcgtgc gcgagccgca ccgagaagta gcccgtgccc
    10921 gagccgatgt cggcgatttt cgcgtccggc gccggcgcca gcgcgcggat cacctcgtgc
    10981 ggcttctgcc aggcgtcgcg ggacgggtcg tcgaagacat gcgcccactt gtcggcgtcg
    11041 gtgaagcggt gctcgtgcgt ggtcggcgac tgcgccgagg ctgcgatcgg gaccgcgagc
    11101 gcggaagcaa ggagccaggc gaggacggcg ctgcgcatgg cccgtattct acgcgtcgct
    11161 gcgccgccgg cgcgcgagca gcgcgcgcag cttcgcgatg tcggcgggct tcaccaggtg
    11221 cgcgtcgaag cccgcctgcg cggcgcgctg cgtgtcctcg ggctgcccgt agccggtgag
    11281 cgcgaccagc gtcgggccgg cgtggcgcgc gcggatcgcc cgcgcgagct cgtagccgtc
    11341 catcccgggc agaccgatgt cgatgagcgc gacgtccggg acgatgcgct cgaacgcggc
    11401 gagaccggcc ttgccgtccg ccgccgtgtg cacggtgtgt ccctcggact cgagcacgat
    11461 ctgcagcgag acgcgcgcgt cggcgttgtc ctcgacgatg aggacctgca gcggtcgcag
    11521 cgcggccggc ggcgccggcg gccgggcgtc cgccgccggc gcctcgaccg cgggcaggcg
    11581 caccgtgaag gtcgcgccgc gccccggccc gtcgctgcgc gccgtcacga tcccgccgtg
    11641 cagctcgacc aggcggtgga cgagcgtgag cccgagcccg aggccgccct tgcggtgcaa
    11701 ctcgctcttg ccctggtaga agaggtcgaa cacgcgcttc agctcgtcgg gcgtcatgcc
    11761 gatgccgtcg tcggcgacct cgagcaccgc gtccccgccc tcgcgccgcg ccgagacgcg
    11821 gatcgagccc cccgcgggcg tatacgcgag cgcgttcgag acgaggttgg tgacgacctg
    11881 ctccatgcgc gtgcggtcgc cgtcgatcca taccggcccg agatcgaacg tcgggcggtg
    11941 gcccgcgaag cggcccgagg cgtggaaggc gtcgagcgtg ccgcgcacgg ccgccgagag
    12001 gtccatgcgc tcgcgctgca ggtagatctt gccggtgatc acgcggccga cgtcgagcag
    12061 atcgtcgatc agccgcgcga ggtgctgcgt ctgccgtgcg atcacgccgc gcgcgaacgc
    12121 gatctgctcg ggcttcgcgc cctcgcggtc gaggacctgc gcggcgttgc tcacggcgcc
    12181 cagcgggttg cgcagctcgt gcccgagcat ggcgaggaac tcgtccttgg agcggttcgc
    12241 cgcttcggcg gccgag
//
LOCUS       DATDMW010000058         4337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_512452, whole genome shotgun sequence.
ACCESSION   DATDMW010000058 DATDMW010000000
VERSION     DATDMW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512452"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VI319_02490"
     CDS             complement(<1..844)
                     /locus_tag="VI319_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6862742.1"
                     /translation="MKRLLLGLLGVAAVALAAALALAYPEYRGDIDAAYARVARGSQI
                     AQTACGPIEYAAMGAGPAVLLVHGAGGGFDQLLDMARALAQRGFRVVAPSRFGYLRTP
                     LPADASPAAQADAHACLLDALKIDRAAVVGVSAGAPSSMQFALRHPERCAALALVVPL
                     AWAPAEERASAPRLSPAAWLVFPTLKSDFLFWLLTRAAPSLVTKTVLGTPPAAVEAAG
                     PAERERIAGMMRLLLPVGPREDGLRNEAAVVRSLERYPLERIEAPTLLVSAADDGYGT
                     YASAR"
     gene            963..1487
                     /locus_tag="VI319_02495"
     CDS             963..1487
                     /locus_tag="VI319_02495"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY6862743.1"
                     /translation="MATLRALLFLGLAAGLAGGCAPDPGPAAVGERFPPLRLAALDSA
                     GRVQEALPRGRNVVLNVWATWCEPCRREMAGLERLAREHGELDVVGVSIDADANLARE
                     FLLAQDVRFPNYIDPGGRAVHDALGVQALPQTFVITRDGTVVARIAGARDWSGRETRS
                     MLRDVLALSSGASP"
     gene            1484..1786
                     /locus_tag="VI319_02500"
     CDS             1484..1786
                     /locus_tag="VI319_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6862744.1"
                     /translation="MKTLAAAIAVLALSTPAAHAADPARGAQIYNVHCASCHGVNGIS
                     VMPTAPNFARSERMLQPDFQLLAAIKAGRAAMPAYNGVLPDRDIYDVIAYMRTLRR"
     gene            1783..2835
                     /locus_tag="VI319_02505"
     CDS             1783..2835
                     /locus_tag="VI319_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY6862745.1"
                     /translation="MSATHVLVAALVLASSPPAAAQRVAESFQVGDNVYVRALAVEPA
                     RNALWIGTSAGVNEIDLGTRARRSTFTRADGLANEYVFAIGVDREGYKWFGTNAGGAS
                     RYKDGKWKTYFPLHGLADYWIYAFANERNGDVWIGTWAGASHFETKTGRFTNYVKELV
                     NEWVYGVAVDAQDRVWFATEGGVSMLERGRWRSWTHKDGLGAPNSGDLPPSANTGLGT
                     RSRHDLSVLAEGAPTYNPSYVFAIHVAPDGAVWAGTWGGGVARWDGKTWKNLSTADGL
                     AGNIVYSIAQDANGVFWFGTDHGASRWDGRSFRNFGTKDGLLAEHVYALAAAGGDVWV
                     GTKRGVARIVNSGARK"
     gene            2832..4049
                     /locus_tag="VI319_02510"
     CDS             2832..4049
                     /locus_tag="VI319_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY6862746.1"
                     /translation="MMRLNKVSVAVTAAAVVALFAGVYWLGARHGERAAGALPQGHPQ
                     VAASADPHQDYREGKAKLDPAKAFTHFRVGDKNVKSIFVEGKVAWVGTSGGVIRYDWG
                     SDDYKLYDARNGLLSNGIFHVGRLQGKIAIGTYGGGLSLLDEKTGTFETYNVPEGLGD
                     AFVYDVLQAKNGDVWIATWSGANRIRGGNLKDRSKWDLYTVENTKHGLPNDWVYGLAE
                     GKNGDIWMATEGGLALLRDGQWTNWNHAKGLGADYDVVKKDIAFDNDPSKQSLHHAQQ
                     KKEMGLSDVSVAYNPNYIIAMKVDAEGVVWAGTWGGGLARWDGKSWKNYTVADGLPGN
                     HVFMLHIDPKGRLWIGTNNGLALLQDGKFVVKTMADGLFAKNVFSMATAADGGQWIGS
                     YGGVAHIRSGLVQ"
     gene            complement(4050..>4337)
                     /locus_tag="VI319_02515"
     CDS             complement(4050..>4337)
                     /locus_tag="VI319_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862747.1"
                     /translation="GDGPGAPSPPRSRPRWRWCSGDAACMGAMIVDARAINDSGAIAA
                     TVCDSNANSHPPSAFDGSTHQPSARACQAVRLDPIRHGANGMTNWPSCSPF"
BASE COUNT          685 a         1478 c         1604 g          570 t
ORIGIN      
        1 agcgcgcgct cgcgtaagtg ccgtagccgt cgtcggcggc cgagacgagc agcgtcggcg
       61 cctcgatgcg ctcgagcgga tagcgctcga gcgaacgcac gacggcggcc tcgttgcgca
      121 ggccgtcctc gcgcggcccc accggcagga ggagccgcat catgccggcg atgcgctcgc
      181 gctcggccgg cccggcggcc tcgaccgccg cgggcggcgt gccgagcacc gtcttcgtga
      241 ccagcgacgg cgccgcgcgc gtcagcagcc agaacaggaa atccgatttc agcgtcggga
      301 acacgagcca cgcggcgggc gacaggcgcg gcgccgacgc gcgctcctcg gccggcgccc
      361 acgcgagcgg caccacgagc gcgagcgcgg cgcagcgctc gggatgccgc agcgcgaact
      421 gcatcgacga gggcgcgccg gccgagacgc cgaccacggc ggcgcggtcg atcttcagcg
      481 cgtcgagcag gcaggcgtgc gcgtcggcct gcgccgcggg cgaggcgtcg gcgggaagcg
      541 gcgtgcgcag gtagccgaac cgcgacggcg cgaccacgcg aaagccgcgc tgcgcgagag
      601 ctcgcgccat gtcgagcagc tggtcgaagc cgccgccggc gccgtgcacg agcaggaccg
      661 cggggccggc gcccatcgcg gcatactcga tcgggccgca cgccgtttgc gcgatctggc
      721 tgccgcgcgc gacgcgcgcg tacgccgcgt cgatgtcgcc gcggtactcc gggtacgcga
      781 gcgcgagggc cgcggcgagc gcaaccgccg cgacgccgag cagcccgagc aggagcctct
      841 tcatggcgcc agcgtacgat gaacgggagg gttcgcgcac gtccacagag tagccccggg
      901 cctccgcccg gatttgatcc aggtcaaatc cggcggccgg gatgggctca atattccggg
      961 ccatggccac gctgcgcgct ttactgttcc tgggcctggc ggccgggctc gccggcggct
     1021 gcgcccccga cccggggccg gccgcggtcg gcgagcgctt tccgccgctg cgcctcgccg
     1081 cgctcgactc cgcgggtcgg gtgcaagagg ccctgccgcg ggggcgaaac gtcgtgctga
     1141 acgtctgggc gacctggtgc gagccctgcc ggcgcgagat ggcgggcctc gagcggctcg
     1201 cgcgcgagca cggcgagctg gacgtggtcg gcgtgtcgat cgacgcggat gcgaacctcg
     1261 cgcgcgagtt cctgctcgcg caggacgtgc gctttcccaa ctacatcgat ccgggcggac
     1321 gcgcggtgca cgacgcgctc ggcgtgcagg cgctgccgca gaccttcgtg atcacgcgcg
     1381 acggcaccgt ggtcgcgcgc atcgccggcg cgcgcgactg gagcggccgc gagacgcggt
     1441 cgatgctgcg cgacgttctc gcgctttcgt caggagcctc gccatgaaaa cccttgcggc
     1501 cgcgatcgcg gtcctggccc tgtccacccc ggccgcgcac gccgcggacc cggcgcgcgg
     1561 cgcgcagatc tacaacgtgc actgcgcctc gtgccacggc gtgaacggca tcagcgtcat
     1621 gccgacggcg ccgaacttcg cgcgctccga gcgcatgctg cagccggact tccagctgct
     1681 cgccgcgatc aaggccggac gcgccgcgat gccggcgtac aacggcgtgc tgcccgaccg
     1741 cgatatttac gacgtgatcg cgtacatgcg gacgctgcgt cgatgagcgc gacgcacgtc
     1801 ctcgtcgcgg cgctggtcct ggcgtcgtcg ccgcccgccg cggcgcagcg cgtcgccgag
     1861 agcttccagg tcggcgacaa cgtctacgtg cgcgcgctcg cggtcgagcc ggcgcgcaac
     1921 gcgctctgga tcgggacctc ggcgggcgtg aacgagatcg acctcggcac gcgcgcgcgg
     1981 cgctcgacct tcacgcgcgc cgatggcctc gccaacgagt acgtgttcgc gatcggcgtc
     2041 gaccgcgaag gctacaagtg gttcggcacc aacgcgggcg gcgcctcgcg ctacaaggac
     2101 gggaagtgga agacgtactt tccgctgcac ggcctcgccg actactggat ctacgccttc
     2161 gcgaacgagc gcaacggcga cgtgtggatc ggcacctggg cgggcgcgag ccatttcgag
     2221 acgaagaccg gcaggttcac caactacgtg aaggagctgg tgaacgaatg ggtgtacggg
     2281 gtcgcggtgg acgcgcagga ccgggtctgg ttcgcgaccg agggcggcgt gtcgatgctc
     2341 gagcgcggcc gctggcgctc gtggacgcac aaggacggac tgggcgcgcc gaactcgggc
     2401 gacctcccgc cgtcggcgaa cacggggctc ggcacgcgct cgcgccatga tctctcggtg
     2461 ctcgccgagg gcgcgccgac ctacaacccg agctacgtgt tcgcgatcca tgtcgcgccc
     2521 gacggcgcgg tgtgggccgg aacctggggc ggcggcgtcg cgcgctggga cgggaaaacg
     2581 tggaagaacc tgagcaccgc cgacggcctc gccggcaaca tcgtctactc gatcgcgcag
     2641 gacgcgaacg gcgtcttctg gttcggcacc gaccacggcg cctcgcgctg ggacgggcgc
     2701 agcttccgca atttcggaac gaaggacggc ctgctcgccg agcacgtgta cgcgctcgcg
     2761 gccgcgggcg gcgacgtctg ggtcggaacg aagcgcggcg tggcgcgcat cgtcaattct
     2821 ggagcgagga aatgatgcgg ctcaacaaag tctccgtcgc ggtcacggcc gcggccgtcg
     2881 tcgcgctctt cgccggtgtg tactggctcg gcgcccgtca cggcgagcgc gcggcgggcg
     2941 cgctgccgca gggccacccg caggtcgcgg cgagcgccga cccgcaccag gattaccgcg
     3001 aaggcaaggc gaagctcgat ccggcgaagg cgttcacgca cttccgcgtc ggcgacaaga
     3061 acgtgaaatc gatcttcgtc gagggcaagg tggcatgggt gggcacctcg ggcggcgtca
     3121 tccgctacga ctggggctcg gacgactaca agctctacga cgcgaggaac ggcctgctct
     3181 cgaacgggat cttccacgtc gggcggctgc aggggaagat cgcgatcggc acctacgggg
     3241 gcggcctgtc gctgctcgac gagaagaccg ggaccttcga gacgtacaac gtccccgagg
     3301 gcctgggcga cgccttcgtc tacgacgttc tgcaggcgaa gaacggcgac gtctggatcg
     3361 cgacctggtc gggcgcgaac cggatccgcg gcggcaacct gaaggaccgc tcgaagtggg
     3421 atctgtatac ggtggagaac acgaagcacg gcctgccgaa cgactgggtg tacgggctcg
     3481 cggaagggaa gaacggcgac atctggatgg cgaccgaggg cgggctcgcg ctgctgcgcg
     3541 acggccaatg gacgaactgg aaccacgcga aggggctggg cgccgactac gacgtcgtga
     3601 agaaggacat cgccttcgac aacgatcctt cgaagcagtc gctgcaccac gcgcagcaga
     3661 agaaggagat gggcctgtcc gacgtcagcg tcgcctacaa cccgaactac atcatcgcca
     3721 tgaaggtcga cgccgaaggc gtcgtctggg cggggacctg gggcggcggc ctcgcgcgct
     3781 gggatggcaa atcgtggaag aactacaccg tcgccgacgg cctgccgggc aaccacgtct
     3841 tcatgctgca catcgatccg aagggcaggc tctggatcgg caccaacaac ggcctcgcgc
     3901 tgttgcagga cggcaagttc gtcgtcaaga ccatggccga cggcctgttc gcgaagaacg
     3961 tgttctcgat ggcgaccgcg gcggacggcg ggcagtggat cgggagctac ggcggcgtgg
     4021 cgcacatccg ctccgggctg gtgcagtagc tagaagggtg agcaggacgg ccagttggtc
     4081 atgccgttgg cgccatgacg gatcggatcc agccggacgg cctggcaggc tcgggcgctc
     4141 ggctggtggg tagaaccgtc gaacgccgac ggcgggtgcg aattcgcgtt cgagtcgcat
     4201 accgtcgcgg cgatggcgcc ggaatcgttg atggcgcgcg catccacgat cattgcgccc
     4261 atgcaggcgg cgtcgccaga gcaccaccgc caccgcggcc gcgagcgggg cggcgatggc
     4321 gcaccgggac catcgcc
//
LOCUS       DATDMW010000059         3689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_886462, whole genome shotgun sequence.
ACCESSION   DATDMW010000059 DATDMW010000000
VERSION     DATDMW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886462"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VI319_02520"
     CDS             <1..477
                     /locus_tag="VI319_02520"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6862748.1"
                     /translation="CAARFPERVEALALIGSAFPMRVSAELLEATRSDEPRARAMINA
                     WSHGGYAQSPGNPGPGSWVIGANLRLMQRAKPGVLHADFSACDDYAAGLERAAQVRCP
                     ALLLSGRRDQMTPARGARALVSAIAGAEWVELDRCGHNVMAEKPDELLDRLVRFLR"
     gene            complement(474..1328)
                     /locus_tag="VI319_02525"
     CDS             complement(474..1328)
                     /locus_tag="VI319_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY6862749.1"
                     /translation="MKLVTWNIQWARGCDGRVDPARIVKAARALADFDVLCVQEVACN
                     FPGLAGSSGEDQFAILGELLPGYSLIRAPAIQVSAPGGAWRQFGNALFTRLPVLQVFR
                     HLLPWPADASVPNMQRSAVEAVVQTRSGPLRVTTTHLEYYSAKQRGAQVERLRELQAE
                     AAGHANEPVRPEKEGSPFAPAPRPASGVLTGDLNFRPDDPLHARMQAPIAPGVPAYRD
                     AWKARHPGRRHDPTLGLFDKQQWPDAPFCCDFIFVTEDLAPRVEQVAVDATTDASDHQ
                     PVLLELRD"
     gene            complement(1342..1770)
                     /locus_tag="VI319_02530"
     CDS             complement(1342..1770)
                     /locus_tag="VI319_02530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6862750.1"
                     /translation="MVDSPHRRFPARMDSMQEIRKFVEEAGTGAGLAREDVLKLLLII
                     EELFTNTVMHGYGGDSNAPVWMSLEPVAAGLRLSYEDEAPAHDPLSSFTPMKTSIMLN
                     EQPVGGLGVKLIRKLATDLSYRREGERNCISLTFTGAARG"
     gene            1834..2289
                     /locus_tag="VI319_02535"
     CDS             1834..2289
                     /locus_tag="VI319_02535"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY6862751.1"
                     /translation="MRPARAVCWRGLLIALLCLAPLVAQAVEDIAQIKVAKGAVFIER
                     GGKRLPAKVGTPVRATDTIITGPDGSVGISFVDNSLLSMGPNSELAIDRFAFNSTTHE
                     GTFNTSLRKGTLAVVSGKIAKQSPTAMKVKLPASILGVRGTVFLVRVEE"
     gene            2296..2892
                     /locus_tag="VI319_02540"
     CDS             2296..2892
                     /locus_tag="VI319_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6862752.1"
                     /translation="MGFAAAIALWVALVVGYPRGGHEMVVLLPDADGKVGTIVVETDK
                     GEQVVLNSAYATARADPVGTVKRETVKEEEIRQTFQSALAAQPKKPVSFTIYFDTGSD
                     ELGDAMKPEIERVLAEMKSRESPEVTVIGHTDLVGANEDNDDLSVKRAERVRDVLVRF
                     GVPADRITVAGRGKREPAVRTSQGVDEPRNRRVVIDVR"
     gene            complement(2889..>3689)
                     /locus_tag="VI319_02545"
     CDS             complement(2889..>3689)
                     /locus_tag="VI319_02545"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY6862753.1"
                     /translation="RRLLRERLQREREAAAQIAGELDAARRIQVGMLPRPETAFPGER
                     RFQLFARMQPAREVGGDLYDFFMLDRDRVFLLIGDVSGKGLPASIFMAVSKALCKRAA
                     LRLGRGVEETLTRVGAEIARENPEALFVTAFAGVLDANTGVLTYSSAGHDAPYLVSPH
                     HGGAERLDQAGGPPLCVVDGHQYAAAGHALQRGDIVCLFTDGITEAMSPEGELYGRER
                     LSAVLERTRAAPSVDMVGQAVLDDVASFVGAAEPADDLTLLVLRWNGA"
BASE COUNT          568 a         1295 c         1285 g          541 t
ORIGIN      
        1 tgcgccgctc ggttccccga gcgcgtcgag gcgctcgccc tgatcggctc ggccttcccc
       61 atgcgcgtga gcgccgagct gctcgaagct acgcgcagcg acgagccccg cgcgcgcgcc
      121 atgatcaacg cctggtcaca cggcggctac gcccagtccc cggggaatcc cgggcctggc
      181 tcgtgggtga tcggcgccaa cctgaggctg atgcagcgcg cgaagccggg cgtgctccac
      241 gccgacttct cggcctgcga cgactacgcc gcgggtctgg agcgggcggc gcaagtgcgc
      301 tgcccggcgc tgctgctgtc gggtcgccgt gatcagatga ccccggcgcg cggcgcgcgc
      361 gcgctggtgt ccgcgatcgc gggcgccgag tgggtcgagc tcgaccggtg cgggcacaac
      421 gtcatggcgg aaaaacccga cgagctgctc gaccggctgg tgcgcttcct gcgctagtcg
      481 cgcagctcga gcagcaccgg ctggtgatcc gacgcgtcgg tggtcgcgtc gaccgccacc
      541 tgctcgaccc ggggcgccag gtcctcggtg acgaagatga agtcgcagca gaacggcgcg
      601 tcgggccact gctgcttgtc gaacaggccg agcgtcggat cgtgccgcct ccccgggtgg
      661 cgcgccttcc acgcgtcgcg atacgccggc acgccgggag ctatcggcgc ctgcatgcgc
      721 gcatgcagcg ggtcgtcggg gcggaaattg aggtcgcccg tgaggactcc ggacgccggc
      781 cgcggcgccg gcgcgaacgg gctgccctcc ttctccggac gcacgggctc gttcgcgtgt
      841 ccggccgcct cggcctgcag ctcgcgcagc cgctcgacct gggcgccgcg ctgcttggcc
      901 gagtaatact cgagatgcgt ggtcgtcacg cgcaggggcc cagagcgcgt ctgcaccacc
      961 gcctcgaccg cggatcgctg catgttcggc acggacgcat cggcgggcca cggcaacagg
     1021 tgacggaaga cctggagcac gggcagccgc gtgaacaggg cattgccgaa ctgccgccac
     1081 gcgccgccgg gggcggacac ctggatcgcc ggcgcgcgga tgagcgagta acccggcaac
     1141 agctctccga ggatcgcgaa ctggtcttcg cccgagcttc ccgcgagtcc cgggaagttg
     1201 caggcgacct cctgcacgca cagcacgtcg aagtccgcca gcgcgcgcgc cgccttcacg
     1261 atgcgggccg gatcgacgcg gccgtcgcag ccgcgcgccc actggatgtt ccaggtgacg
     1321 agcttcaccg cgcgagtccg gctaaccgcg cgcggcgccc gtgaacgtca ggcttatgca
     1381 attgcgctcg ccctcccgcc ggtagctgag gtcggtggcg agcttgcgga tcagcttgac
     1441 gcccagcccg ccgacgggct gctcgttcag catgatcgag gtcttcatcg gcgtgaacga
     1501 gctcagggga tcgtgcgccg gagcctcgtc ctcgtagctc agcctgaggc cggccgcgac
     1561 cggctcgagc gacatccaca caggcgcatt gctgtcgccg ccatagccgt gcatgaccgt
     1621 gttggtgaac agctcctcga tgatgagaag cagcttgagg acgtcttcgc gcgcgagccc
     1681 ggcgcccgtc ccggcctctt ccacgaactt gcggatctcc tgcatcgaat ccatgcgggc
     1741 cgggaaccgc cggtgcggtg aatcgaccat gatcggagcc gggaagggtc gaattggcta
     1801 ttataggaac aaacccaggg agattcgatc atcatgcggc ccgcccgcgc cgtctgctgg
     1861 cgcggacttc tcatcgccct cctgtgcctc gcgcctctcg tggcacaggc ggtggaggac
     1921 atcgctcaga tcaaggtcgc caagggcgcg gtgttcatcg agcgcggcgg caagcgcctg
     1981 cccgcgaagg tcgggacgcc cgttcgcgcc accgacacga tcatcaccgg ccccgacggc
     2041 tcggtcggca tctcgttcgt ggacaactcg ctgctctcca tggggccgaa cagcgagctc
     2101 gccatcgacc ggttcgcatt caactcgacg actcacgaag ggacgttcaa cacgtcgctg
     2161 cgcaagggga cgctcgccgt cgtatcgggc aagatcgcca agcaatcgcc gaccgcgatg
     2221 aaggtgaagc tgccggcgtc catcctgggc gtgcgcggca ccgtattcct ggtgcgggtg
     2281 gaggagtaga cggacatggg attcgcggct gcaatcgctc tgtgggtggc cctcgtggtc
     2341 ggatatcccc gcggcggcca cgagatggtg gtgctgcttc cggacgcgga cggcaaggtc
     2401 ggcaccatcg tggtcgagac cgacaaaggc gagcaggtcg tgctcaacag cgcctacgcc
     2461 accgcccggg ccgaccccgt cggcacggtc aaacgggaaa ccgtcaagga agaggaaatc
     2521 aggcagacgt tccagtctgc gctcgccgcg cagccgaaga agccggtgtc gttcacgatc
     2581 tatttcgaca cgggaagcga cgagctgggc gatgcgatga agcccgagat cgagcgcgtc
     2641 ctcgcggaga tgaagagccg ggagagtccc gaggtgaccg tgatcgggca caccgacctc
     2701 gtcggcgcca acgaggacaa cgacgatctg tcggtgaagc gggccgagcg ggttcgcgat
     2761 gtcctggtcc ggttcggcgt ccccgccgat cgcatcacgg tcgcagggcg cggcaagcgc
     2821 gagcccgccg tgcggacttc gcagggcgtc gacgagcccc gcaaccggcg cgtcgtgatc
     2881 gacgtccgct aggcgccgtt ccagcgcagc acgagcagcg tcaggtcgtc cgcgggctcc
     2941 gcggccccga cgaagctggc cacgtcgtcc agcacagcct gccccaccat gtcgacggaa
     3001 ggtgcagcgc gcgtgcgctc gagcaccgcg ctcaggcgct cgcggccgta gagctctccc
     3061 tccgggctca tcgcctcggt gatgccatcg gtgaacaggc agacgatgtc gccgcgctgc
     3121 agcgcgtggc ccgcggcggc gtactggtgc ccgtcgacca cgcacagcgg cgggccgccc
     3181 gcctggtcga gccgctcggc gcccccgtga tggggcgaca ccaggtaggg agcgtcgtgc
     3241 ccggcgctcg agtacgtcag cactcccgtg ttcgcgtcca ggacgcccgc gaaggcggtg
     3301 acgaacagcg cctcggggtt ctcgcgcgcg atttccgcgc cgacgcgtgt cagcgtctcc
     3361 tccacgcccc ggccgagccg gagcgcggca cgcttgcaca gcgccttgct cactgccatg
     3421 aagatgctcg cgggcaggcc ttttcccgac acgtcgccga tcagcaggaa gacgcgatca
     3481 cggtcgagca tgaagaaatc atacaggtcg ccgcccacct cgcgcgcggg ctgcatgcgc
     3541 gcgaacagct ggaaccgccg ctcgccgggg aacgcggttt cgggcctggg cagcattccg
     3601 acctggattc gtcgcgccgc gtcgagctcg ccggcgatct gcgcggccgc ctctcgctcg
     3661 cgctgcagcc gctcgcgcag caggcgccg
//
LOCUS       DATDMW010000060         2199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1634088, whole genome shotgun sequence.
ACCESSION   DATDMW010000060 DATDMW010000000
VERSION     DATDMW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634088"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VI319_02550"
     CDS             complement(<1..631)
                     /locus_tag="VI319_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6862754.1"
                     /translation="MHRSVVIAAAAALAVASVAAAAQPSPLTLAEAQRRAVERSRALG
                     AQDAAIAASREMAVAAGELPDPVLKLQLQNVPVEGPDRFTLNRDFMTMRAVGVMQEWT
                     RREKRDLKRERFEREAQKDEAQKSVDIANIQRDTALAWLDTYYAQAMVGVIAEQAASS
                     QLEIAAAESAYRAGRVNQADVIAAHSARTGLDDRVSEFARKLRNARTALA"
     gene            complement(704..1093)
                     /locus_tag="VI319_02555"
     CDS             complement(704..1093)
                     /locus_tag="VI319_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862755.1"
                     /translation="MSRRTRPFAALFAIVALLFAQAMAAVHACDVLAPEKAPVTASAP
                     AQAGDDCCDHDTLPADPACGNHCQQGNQAPDRVQPTVAPLLALGFMMTSVAIAPAPVA
                     SHPATAAPDLERDTQPPISIRNCCFRI"
     gene            complement(1140..>2199)
                     /locus_tag="VI319_02560"
     CDS             complement(1140..>2199)
                     /locus_tag="VI319_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6862756.1"
                     /translation="DRALAAAPDAPETHNNRGVALAALNRHREAIASYSRALELRPAY
                     DRARFNRAMSRLVTGDFAGGWKDHESRWTGSEMHQGPRRLPGRAWDGREDLRGKTLLL
                     YAEQGLGDAIQFARYATLAARRGARVLLEAHAPLKELLARIEGVAGAFALGEPLPAFD
                     LHCALMSLPLAFGTTLGTIPHDTPYLAAPAEHAAKWRERLRGCEGLRVGLAWSGSRTL
                     RNDANRSIALSRLAALAAPGRMLVSVQKDIREPDRAIMAQLGIVSFEAELGDFRDTAG
                     LIDALDVVVSVDTAAAHLAGAMGKATWVLLPFSPDWRWLLGRDDSPWYPTARLFRQPA
                     RGDWETPLNALDEALRKL"
BASE COUNT          284 a          772 c          831 g          312 t
ORIGIN      
        1 gcgcgagcgc ggtcctcgcg ttgcgaagct ttcgcgcgaa ctcgctgaca cggtcgtcga
       61 ggccggtgcg cgcgctgtgc gcggcgatga cgtcggcctg gttcacgcgt cccgcgcgat
      121 aggcgctctc ggccgccgcg atctcgagct gcgaggaggc cgcctgctcg gcgatcacgc
      181 ccaccatggc ctgcgcgtag taggtgtcga gccacgcgag cgcggtgtcg cgctggatgt
      241 tcgcgatgtc cacgctcttc tgcgcttcgt ccttctgcgc ctcgcgctcg aagcgctcgc
      301 gcttgaggtc gcgcttctcg cgccgcgtcc attcctgcat gacgcccacc gcgcgcatcg
      361 tcatgaagtc gcggttcagc gtgaagcggt ccggcccctc gaccggcacg ttctgcagct
      421 gcagcttgag gaccggatcg ggcagctcgc cggcggccac ggccatctcg cgcgaggcgg
      481 cgatggcggc gtcctgcgcg cccagcgcgc gcgagcgctc gaccgcgcgg cgctgcgcct
      541 cggcgagcgt gagcggcgac ggctgcgcgg cggcggcgac ggatgccacg gcgagggcgg
      601 cggcggccgc gatgacgaca cttcgatgca tggaacctcc ggcgacggga cgacgggacg
      661 cgcgcgggcg gggcgcgcga gactcaggac cgcggtcggg aggtcagatc cggaagcagc
      721 agttccggat ggagatcgga ggttgcgtgt cgcgctcgag atcgggcgcg gcggtggctg
      781 ggtgcgaggc gacgggcgcc ggcgcgatcg ccacgctcgt catcatgaag cccagcgcga
      841 gcagcggcgc gaccgtgggt tgcacgcggt cgggcgcctg gttgccctgc tggcagtgat
      901 tgccgcaggc cggatcggcc ggcagcgtgt cgtggtcgca gcaatcgtcg ccggcctgcg
      961 ccggtgccga agccgtcacg ggcgccttct ccggcgccag cacgtcgcac gcatgcaccg
     1021 ccgccatcgc ttgcgcgaac agcagcgcga cgatggcgaa gagggcggcg aaaggacgcg
     1081 tacggcggct catggcttgc agtataggtt acacccgccc tggtgccgtt tgatccgact
     1141 caaagcttcc ggagggcctc gtccagcgca ttgaggggtg tttcccagtc gccgcgggcc
     1201 ggctggcgga agagccgcgc cgtgggatac cacgggctgt cgtcgcggcc gaggagccag
     1261 cgccagtcgg gggagaaggg cagcaggacc cacgtggcct tccccatggc gccggcgagg
     1321 tgtgcggcgg cggtgtccac gctcacgacg acgtcgagcg catcgatgag gcccgcggtg
     1381 tcgcggaaat cgccgagctc ggcctcgaag ctcacgatgc cgagctgcgc catgatggcg
     1441 cgatccggct cgcggatgtc cttctgcacg ctcaccagca tgcggcccgg cgcggcgagg
     1501 gccgcgaggc gcgaaagcgc gatcgagcgg ttcgcatcgt tgcgcagtgt ccggctcccg
     1561 gaccaggcga gccccacgcg caggccttcg cagccgcgca ggcgttcacg ccacttcgcg
     1621 gcgtgctccg cgggcgccgc gaggtacggc gtgtcgtgcg gaatggtgcc gagcgtcgtc
     1681 ccgaaggcga gcggcaggct catcagcgcg cagtgcaggt cgaaagcggg cagcggctcg
     1741 cccagcgcga aggcgccggc cacgccctcg atccgcgcga gcagctcctt cagcggcgcg
     1801 tgcgcttcga gcagcacgcg cgcgccgcgc cgcgcggcga gcgtcgcata gcgcgcgaac
     1861 tggatcgcgt cgcccaggcc ctgctcggcg tagaggagca gcgtcttgcc gcgcaggtcc
     1921 tcgcgtccgt cccacgcgcg tcccggcagc cggcgcggcc cctggtgcat ctcgctgccg
     1981 gtccagcggc tctcgtggtc cttccagcct ccggcgaaat cccccgtcac cagccgcgac
     2041 atcgcgcgat tgaagcgcgc gcggtcgtac gcggggcgca actcgagcgc gcgcgagtag
     2101 ctcgcgatcg cctcgcggtg gcgattgagc gccgccagcg ccacaccgcg gttgttgtgc
     2161 gtttccggcg cgtccggcgc tgcggcgagc gcgcggtcg
//
LOCUS       DATDMW010000061        11359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1136550, whole genome shotgun sequence.
ACCESSION   DATDMW010000061 DATDMW010000000
VERSION     DATDMW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136550"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VI319_02565"
     CDS             complement(<1..396)
                     /locus_tag="VI319_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEY6862757.1"
                     /translation="MRTALPVSVLAFAASLACSLVAQAQQGPGSAAPAAAPPSWQQGR
                     NPEQSESPLHPFAPHMTGRPAKDLPINTLKVPKGFKVEVWQDGIPEARSLALGDKGTV
                     FVSNRLSKNVYAVVDRNGRREVKTVLKGLD"
     gene            527..1333
                     /locus_tag="VI319_02570"
     CDS             527..1333
                     /locus_tag="VI319_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY6862758.1"
                     /translation="MTRICSLTELAAAIPDGARLAIPPFYASPAMAATRELLRRGVRD
                     LHLVCVPQSDIQADLLIGAGAVRVVETSAVTLGELGSAPRFTAAVRSGALRLVDATCP
                     AIHAGLAAAQKGVPFMPIRGILGSDLLAHRPDWKVIENPFAAGDAIVAVPAIRPDVAL
                     FHAPLADRDGNVFIGRSRELLNMAQASATSCVTVEEIADGDLLADPARSGAVVPAIYI
                     GRVAVARRGAAPHALAGAYDADEAFLARDAAAARSEDGFRAFVDEWLGAP"
     gene            1330..2088
                     /locus_tag="VI319_02575"
     CDS             1330..2088
                     /locus_tag="VI319_02575"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY6862759.1"
                     /translation="MSGSYAPSELLIAVIARLLEGCRHVAVGSQSPIPGSAALLAREL
                     SRADPGRGEREPMRVSVLGSRRNNFFTSGGVELFDLAAQGRIDAFFLGGGQIDGEANI
                     NLTGTGDWPGTQVRWPGAFGSAFLYFLVPRVILFREEHTRRTFVPRVDIVTAPGTSRE
                     GVYRPGGPHALLTSLALFSFDQARRRFRIESVHPGHTLEEVLDNTGFDFDRPPLVPQT
                     PPPEPERLALIRGPVRDEIAEIYPRFALSLAASR"
     gene            complement(2049..2717)
                     /locus_tag="VI319_02580"
     CDS             complement(2049..2717)
                     /locus_tag="VI319_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862760.1"
                     /translation="MFIGHFAVAMAAKRAAPRTSLGTLTLAAQLLDLLWPLLLLAGVE
                     TVEIRPGDTAFTPLAFTHYPVSHSLATALGWAAGFAVLYYAATRYGRGALLVAVLVVS
                     HWVLDWITHRPDLPLWPGGPLQGLGLWNSVAATLVVETLVFAAGLAVYLRATRARDPL
                     GRWNAVAFAAFLFAAYLAAAFGPPPPDVRTLAYSAFAVWLLVAWAAWVDRHREAASDK
                     AKRG"
     gene            complement(2718..3299)
                     /locus_tag="VI319_02585"
     CDS             complement(2718..3299)
                     /locus_tag="VI319_02585"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEY6862761.1"
                     /translation="MRAAMRAFSVALCLAWLQVAAAAEVAGVRIEDKVRVGASELVLN
                     GAGLRKRIVFKVYVGALYLPEKTGDAARVIASSGPKRVSMTLMRDLTAKQLTEALEDG
                     MRANHTEAQLAALRSRLDTAQATMREIGSAKEGSVIALEYLPGTGTRITVDGTPKGPT
                     IPGEDFYAALLRVWLGDKPVDATLKKAMLGQGG"
     gene            complement(3300..4469)
                     /locus_tag="VI319_02590"
     CDS             complement(3300..4469)
                     /locus_tag="VI319_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY6862762.1"
                     /translation="MDLSKATFNGYKRENGRVGVRNHVIILPVDDLSNAAAEAVAHNV
                     KGTMALPHPYGRLQFGADLELHFRTLIGTGANPNVAAVVVICIEPGWAKKVADGIART
                     GKPVAYFGIEQHGDHDTIMRASKVAKEYVQWASELRREERPLKDLWVSTKCGESDTTS
                     GIGANPCVGNAFDKLYANGVTLVFGETTELTGGEQLVAARCRTPKVREDFMRMFDRYQ
                     EVVERHKTSDLMDSQPTKGNIEGGLTTIEEKALGNIQKIGKQCRIDGVLDKAETPSGP
                     GLWFMDSSSAAAEMVTLCAASGYVVHFFPTGQGNVIGNPILPVIKLCANVRTVRTMSE
                     HIDVDVTGITRKELNMDQAGDKLLEMMFRTANGRLTAAEALGHREFVLTRLYESA"
     gene            complement(4473..4754)
                     /locus_tag="VI319_02595"
     CDS             complement(4473..4754)
                     /locus_tag="VI319_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HEY6862763.1"
                     /translation="MIHFVVHDEGDSVGVVVVEGVKSGQELTGWIMDQDRELKVRAKN
                     DIPIGHKLAIRALSKDATVIKYGTDIGRAVAAIQVGEHAHVHNIKTKRW"
     gene            complement(4762..5046)
                     /locus_tag="VI319_02600"
     CDS             complement(4762..5046)
                     /locus_tag="VI319_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862764.1"
                     /translation="MVRGSRTLLLGILVLPPIAGCVISYRGDVPARNVVAGAMIVGIV
                     AAGEDRYYRVGPDGMRTPVRPAPPPDPTRKINVQDCTEPVDWSSGNLVCK"
     gene            5171..6400
                     /locus_tag="VI319_02605"
     CDS             5171..6400
                     /locus_tag="VI319_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862765.1"
                     /translation="MNKPIDARPADTRDYVLSRTDKYFTKSRQIIEKFGDRAVTYGVF
                     MRRGVICAVNPAIDVLRRHYPVVDGSAPELKITRLYEEGAFVPDQKPLFTYTGSFAAL
                     VELETLILQRVGMPCVAAYNAYKMAMNLKKTAFIDMHARHATGDDMSLLAAYGASVGS
                     RMAKLAGAVGFTGSSQDLSAHLYGAEKAVGTMPHAIVGYAGSTLRAAQMYVEAHPKDD
                     LTVLIDYFGREYTDALEVCRWWTHEVLPKDPSGSRRLGLRIDTHGERYAEGLDYEKSV
                     DIVVSWLHVPNEYEAVRHVMGEEAFDADSLNIVKDRVRKVLFGTGVSVAAVIQLRQTL
                     DRNGFNAAKIVASSGFNLFKCKMFANARAPVDVVGTGSFLPDTLADTFATADVFLYEG
                     KPGIKLGREKLFQGLKA"
     gene            6402..7427
                     /locus_tag="VI319_02610"
     CDS             6402..7427
                     /locus_tag="VI319_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862766.1"
                     /translation="MLLPTTLVGSYPQPEWLIDREKLAGRFPPRVRAKELWRIPEPYL
                     AQAQDDATLLAIRAQEDAGLDIVTDGEIRRESYSNRFATALEGIDLDNPGTALDRSGH
                     PNPVPRIVGKIRRRHPVELEDLKFLRAHTRRMIKMTVPGPFTLSQQAQNDYYKDAAEA
                     AMDYAAAVNEEIRDLFAAGADIVQVDEPYMQARPEKAREYGLAALNRALEGITGTTAV
                     HICFGYAAVIHVRPSGYSFLGELAGCTCKQVSIETAQSNLDCAVLKTLPGKKIILGVI
                     DLNDMKVETPETVAARIRRALPYVKAEDVIVAPDCGMKYLPRDVAFGKMTAMAEGARI
                     ARAELTR"
     gene            complement(7424..8602)
                     /gene="metC"
                     /locus_tag="VI319_02615"
     CDS             complement(7424..8602)
                     /gene="metC"
                     /locus_tag="VI319_02615"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEY6862767.1"
                     /translation="MRDETVIVHSGRHPEQYEGAVNPPVYHASTILARSAEEYLARRR
                     AWLDEVPGTYYGRFGTPTVEALQEAIAELEGGHRAVVYPSGLAACAGALTAFLSAGDH
                     VLMSDSTYGPTRNFAKGTLKRFGVTTTFFDPLVGRGIESLIQGNTRVVYLESPGSLTF
                     EVQDVPAIADVAHRRGIVVIMDNTWATPLYFKALAHGVDVSVQAATKYVVGHSDAMLG
                     TVACTKEAWPRLRTSTYEMGQTTGPDVVYLGQRGLRTLAARLRIHSENARALTGWLAK
                     QPEVDRVLYPALPSDPGHALWKRDFTGASGLFGVVLKRGIDQKAMFALIDGMKLYGIG
                     ASWGGYESLIVPYHPMEGRAVTRWPEHGPCFRVHAGLESSDDLLEDLEAGFVRLRRNA
                     "
     gene            8725..10128
                     /locus_tag="VI319_02620"
     CDS             8725..10128
                     /locus_tag="VI319_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY6862768.1"
                     /translation="MTLPVSRAVRSIGRLAWPILVAQLAVMASGVLDTVMSGHYSAVD
                     LAAVGIGASIYYSIFIGLLGVVQSLSPIAAQLFGAKQYARIGEEARQTMWLATGLALC
                     GVLLLGFPEPFLRLAGAPPEVEARVRLYLVGIAWALPGMLLFRVFFAFTTAVSRPRAA
                     MALNLFYFCAKLPLNWVFMYGKLGAPELGGPGCAVATAVVAWVAALIGWAYCARDPFY
                     RPFRVFAQWSWPEARVLWSHLKLGVPMGLSFFVEVTSFTFIAIFVARLGATTSASHQI
                     AANVSAVVYMFGLAIGNATGVLVAQAVGARSRHDARRTGLTGMAIMMLISGCASLALV
                     PGGAGIAGLYTDDPGVRTLATRLLAFVAFYQLFDGVQVVIVNALRGYRIAFLPMLVYT
                     VALWGIGLGGGYVLGLRGSDAARSLGLATPMGAAGFWLAGAFGLIVTGAILFAYFLRA
                     SAVRPDGRTAEANVGTA"
     gene            complement(10125..10679)
                     /locus_tag="VI319_02625"
     CDS             complement(10125..10679)
                     /locus_tag="VI319_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6862769.1"
                     /translation="MLDSFRRRDSSRVARYQSPYGELQEALTPSQLADQVPANKPEMK
                     APETNPQATMQNEKAPEQVSKLIVGPDIKLKGAEITDCDTLVVEGRVEASMDSRVIQI
                     AENGVFVGKVGIDIAEIRGRFDGELTARKQLVIRSTGRVSGKIRYGKVAIEEGGELSG
                     DIGAITDTGAAAQPLFPKSANKAA"
     gene            10968..>11359
                     /locus_tag="VI319_02630"
     CDS             10968..>11359
                     /locus_tag="VI319_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009980621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY6862770.1"
                     /translation="MRNLRTRDATLIALALLAGCVATDTTRTPALARIENVVVIYAEN
                     RSFDHLYGLFPGAEGIADATPAQKTQLDLDGRPLPHLPPTYEGGKVRADLPTQTLPNA
                     PFRIDAPPIGRRWDEVLPSPIHAYFQNR"
BASE COUNT         1621 a         4012 c         3896 g         1830 t
ORIGIN      
        1 gtcgagcccc ttcagcacgg tctttacctc gcgccggccg ttgcggtcga ccaccgcgta
       61 cacgttcttc gagaggcggt tgctgacgaa caccgtgccc ttgtcgccga gggcgagcga
      121 gcgcgcctcg gggatgccgt cctgccacac ctcgaccttg aagcccttgg gcaccttcag
      181 cgtgttgatg ggcagatcct tggccggacg cccggtcatg tgcggcgcga aggggtgcag
      241 gggcgactcc gactgctcgg ggttgcggcc ctgctgccag gatggcggcg cggcggcggg
      301 ggcggcgctg ccggggccct gctgcgcctg ggcgacgagc gagcaggcga gcgaggcggc
      361 gaatgcaaga acggaaacgg gaagggcggt gcgcataact cctcctggtt cgaatctcga
      421 gacgtttcga cgggagctgc gatgcaaatt gcggtgtgcg atggcatcat agccacgacc
      481 gatggccgcc gcaaccggcc gatgctgccg tcatgaatcc cgccccgtga cccggatctg
      541 ctccctgacc gagctcgccg ccgccattcc cgacggcgcc aggctcgcca tccccccctt
      601 ctatgcgagt cccgcgatgg cggcgacgcg cgagctgctg cgccgcggcg tgcgcgacct
      661 gcacctcgtg tgcgtgccgc agagcgacat ccaggccgac ctcctgatcg gcgccggagc
      721 ggtgcgcgtg gtcgagacgt cggccgtgac gctgggggag ctggggtcgg cgccgcgctt
      781 caccgcggcg gtgcgctcgg gcgccttgcg cctggtcgac gccacctgcc cggcgatcca
      841 cgccggcctc gccgccgcgc agaaaggcgt gccgttcatg cccatccgcg gcatcctcgg
      901 cagcgacctg ctcgcgcacc gtcccgactg gaaggtgatc gagaacccgt tcgccgccgg
      961 cgacgcgatc gtcgcggtgc ccgcgatccg gcccgacgtc gcgctcttcc acgccccgct
     1021 cgccgaccgc gacggcaacg tcttcatcgg ccgcagccgc gagctgctca acatggcgca
     1081 ggcctcggcg acgagctgcg tgacggtgga ggagatcgcc gacggcgatc tgctcgccga
     1141 tcccgcgcgc tcgggcgccg tcgtgccggc catctacatc ggccgggtcg cggtcgcgcg
     1201 ccggggcgcc gcgccgcacg cgctcgccgg cgcctacgac gccgacgaag ccttcctcgc
     1261 gcgcgacgcc gcggcggcgc gcagcgagga cggcttccgc gccttcgtcg acgagtggct
     1321 gggtgcgccg tgagcgggag ctacgccccg tccgagctgc tgatcgcggt gatcgcgcgg
     1381 ctgctcgagg gctgccgcca cgtcgcggtc gggtcgcagt cgccgatccc gggctcggcg
     1441 gcgctgctcg cgcgcgagct ctcgcgtgcc gatccggggc gcggcgagcg cgagccgatg
     1501 cgcgtctcgg tgctcggctc gcgccgcaac aacttcttca cctcgggcgg tgtcgagctg
     1561 ttcgacctcg ccgcgcaggg acgcatcgac gccttcttcc tcgggggcgg ccagatcgac
     1621 ggcgaggcga acatcaacct caccggcacc ggcgactggc cggggacgca ggtgcgctgg
     1681 ccgggcgcgt tcggctcggc gtttctgtat ttcctcgtgc cgcgcgtgat cctgttccgc
     1741 gaggagcaca cccgccggac gttcgtcccg cgggtcgaca tcgtgaccgc gccggggacg
     1801 agccgcgaag gggtttatcg gccgggcggc ccgcacgcgc tgctgacgag cctcgcgctc
     1861 ttctcgttcg accaggcgcg gcggcgcttc cgcatcgaga gcgtgcaccc cggacacacg
     1921 ctcgaggagg tgctcgacaa caccgggttc gacttcgatc gcccgccgct cgtcccgcag
     1981 acgccgcccc cggagcccga gcggctcgcg ctgatccgcg gcccggtgcg cgacgagatc
     2041 gccgagatct atccgcgctt cgccttgtcg ctcgcggctt cgcggtgacg gtcgacccat
     2101 gccgcccagg cgacgagcag ccacaccgcg aaggccgagt acgcgagcgt gcgcacgtcg
     2161 ggcggcggcg gtccgaacgc cgcggcgaga tacgcggcga agaggaacgc cgcgaacgcg
     2221 accgcgttcc agcgacccag gggatcgcgc gcgcgcgtcg cgcgcagata gaccgcgagc
     2281 ccggcggcga acacgagcgt ttccacgacg agcgtcgcgg cgaccgagtt ccacagtccc
     2341 aggccttgca gcgggccgcc cggccagagg ggcaggtcgg ggcgatgcgt gatccagtcg
     2401 agcacccagt ggctgacgac gagcactgcg acgaggagcg cgccccgtcc gtagcgcgtt
     2461 gcggcgtagt acagcaccgc aaagccggcc gcccagccga gcgcggtcgc aaggctgtgc
     2521 gagaccgggt agtgcgtgaa ggcgagcggc gtgaacgcgg tgtcgcccgg ccggatctcg
     2581 accgtctcga cccccgcgag cagcagcagc ggccacagca gatcgaggag ctgcgcggcg
     2641 agcgtgaggg tgcctagcga ggtccgcggc gccgcgcgtt tcgccgccat cgccaccgcg
     2701 aagtggccga tgaacatcta gccgccctgg cccagcatcg ccttcttgag cgtcgcgtcg
     2761 accggcttgt cgccgagcca gaccctgagc agcgcggcgt agaaatcctc cccggggatc
     2821 gtcggcccct tgggcgtgcc gtcgacggtg atgcgcgttc cggtccccgg caggtactcg
     2881 agcgcgatga ccgagccctc cttcgccgag ccgatctcgc gcatggtcgc ctgcgccgtg
     2941 tcgaggcgcg agcgcagcgc cgcgagctgc gcctcggtgt ggttggcgcg catgccgtcc
     3001 tcgagcgcct cggtgagctg cttcgccgtc agatcgcgca tcagcgtcat cgacacgcgc
     3061 ttcgggccgc tcgacgcgat gacccgggcc gcgtcgcccg tcttttccgg caggtacagc
     3121 gcgccgacgt agaccttgaa cacgatgcgc ttgcgcaggc ccgcgccgtt cagcaccagc
     3181 tcgcttgcgc cgacccgcac cttgtcctcg atccgcacgc cggccacctc ggccgcggcg
     3241 gcgacctgga gccaggcgag gcagagcgcg accgagaagg ctcgcatcgc ggcgcgcatc
     3301 tacgcgctct cgtacagccg cgtcagcacg aactcgcggt gcccgagtgc ctcggccgcg
     3361 gtcagccgtc cgttcgcggt gcggaacatc atctcgagca gcttgtcgcc cgcctggtcc
     3421 atgttgagct ccttgcgggt gatgccggtg acgtcgacgt cgatgtgctc ggacatggtg
     3481 cgcaccgtgc ggacgttggc acacagcttg atgaccggca gaatcgggtt gccgatgacg
     3541 ttgccctgac cggtcgggaa gaagtgcacc acgtagccac tcgccgcgca cagcgtcacc
     3601 atctcggcgg cagccgagga cgagtccatg aaccacagcc cgggcccgct cggcgtctcg
     3661 gccttgtcga gcacgccgtc gatcctgcac tgcttgccga tcttctggat gttgccgagc
     3721 gccttctcct cgatcgtcgt caggccgcct tcgatgttgc ccttggtcgg ttgcgaatcc
     3781 atgaggtcgc tcgtcttgtg gcgctcgacc acttcctggt agcggtcgaa catgcgcatg
     3841 aagtcttcgc gcaccttggg cgtgcggcag cgcgccgcca ccagctgctc gccgcccgtg
     3901 agctcggtgg tctcgccgaa gacgagcgtc acgccgttcg cgtagagctt gtcgaaagcg
     3961 ttgccgacgc aggggttggc gccgatgccc gaggtggtgt ccgactcgcc gcacttggtg
     4021 gagacccaga gatccttcag cggacgctcc tcgcggcgca gctcgctcgc ccactgcacg
     4081 tattccttcg ccaccttcga ggcgcgcatg atggtgtcgt ggtcgccgtg ctgctcgatg
     4141 ccgaagtagg cgacgggctt gcccgtcctc gcgatgccgt cggcgacctt cttcgcccag
     4201 cccggctcga tgcagatcac cacgaccgcg gcgacgttgg ggttcgctcc ggtgccgatc
     4261 agcgtgcgga agtgcagctc gagatcggcg ccgaactgca gccgcccgta cgggtgcggc
     4321 agcgccatcg tgcccttgac gttgtgcgcg accgcctcgg cggccgcgtt cgacagatcg
     4381 tcgacgggga ggatgatgac gtggttgcgc acgccgacgc gcccgttctc gcgcttgtag
     4441 ccgttgaatg tcgctttgct cagatccatc gcctaccacc gcttcgtctt gatgttgtgc
     4501 acgtgcgcgt gctcgcccac ctggatggcg gcgaccgcgc ggccgatgtc ggtgccgtac
     4561 ttgatcaccg tcgcgtcttt cgagagcgcg cggatcgcga gcttgtggcc gatcgggatg
     4621 tcgttcttcg cccgcacctt gagctcgcgg tcctggtcca tgatccagcc ggtgagctcc
     4681 tgcccggatt tcaccccttc gacgacgacc acgcccaccg agtcgccctc gtcgtgcacc
     4741 acgaaatgaa tcatgctctc ctcacttgca caccaggttg cccgacgacc agtccaccgg
     4801 ctcggtgcag tcctggacgt tgatcttccg cgtcgggtcc ggcggcggcg ccgggcgcac
     4861 cggcgttcgc atcccgtcgg gtccgacgcg atagtagcgg tcctcgccgg ccgcgacgat
     4921 cccgacgatc atcgcgccgg cgaccacgtt gcgcgcgggc acgtcgcccc ggtagctgat
     4981 gacgcacccg gcgatcggcg gcaggacgag gatgccgagg agcaatgtgc gggacccgcg
     5041 gaccatgcct gcgaggctag cacgggccgt gcgcggaggc cagatgcggc gcccctgcta
     5101 gaatgtccgg gttcctcggc tcccgcgtca acgccgcggt caatgccgcg gtcaacggaa
     5161 tggatcgccc atgaacaagc ccatcgacgc ccggcccgcc gacacgcgcg attacgtgct
     5221 cagccggacc gacaagtact tcaccaagtc gcggcagatc atcgagaagt tcggcgaccg
     5281 cgcggtcacc tacggtgtgt tcatgcggcg cggcgtgatc tgcgccgtga acccggcgat
     5341 cgacgtgctg cgccggcact accccgtcgt cgacggaagc gcgcccgagc tgaagatcac
     5401 gcgcctgtac gaggagggcg cgttcgtccc ggaccagaag cccctgttca cgtacaccgg
     5461 ctcgttcgcc gcgctggtcg agctcgagac gctgatcctg cagcgcgtcg gcatgccgtg
     5521 cgtggccgcc tacaacgcct acaagatggc gatgaacctg aagaagaccg cgttcatcga
     5581 catgcacgcg cgccacgcga cgggcgacga catgtcgctg ctcgcggcct acggggcctc
     5641 ggtcggcagc cgtatggcga agctcgccgg cgcggtcggc ttcaccggct cgtcgcagga
     5701 cctctcggcg catctctacg gcgccgagaa ggcggtcggc accatgccgc acgcgatcgt
     5761 cggctacgcc ggctcgaccc tgcgcgccgc gcagatgtac gtcgaggcgc acccgaagga
     5821 cgacctgacc gtgctcatcg actacttcgg gcgcgagtac accgacgcgc tcgaggtctg
     5881 ccgctggtgg acccacgagg tgctgccgaa ggacccgagc ggctcgcgcc gcctggggct
     5941 gcgcatcgac acgcacggcg agcgctacgc cgaggggctc gactacgaga agtcggtcga
     6001 catcgtcgtc agctggctgc acgtgccgaa cgagtacgag gcggtgcgcc acgtcatggg
     6061 cgaggaggcg ttcgacgccg acagcctgaa catcgtcaag gaccgcgtgc gcaaggtgct
     6121 cttcggcacc ggcgtctcgg tcgccgcggt gatccagctg cgccagacgc tcgaccggaa
     6181 cggcttcaac gccgcgaaga tcgtcgcctc ctcgggcttc aacctgttca agtgcaagat
     6241 gttcgcgaac gcgcgcgcgc cggtcgacgt cgtcggcacg ggctcgttcc tgcccgacac
     6301 cctcgccgac accttcgcca ccgcggacgt cttcctctac gaaggcaagc ccgggatcaa
     6361 gctcgggcgc gagaagctgt tccagggcct gaaggcctga cgtgctgctt cccacgacgc
     6421 tggtcggcag ctacccgcag ccggagtggc tgatcgaccg cgagaagctc gcggggcgct
     6481 tcccgccgcg cgtgcgcgcg aaggagctct ggcgcatccc cgagccgtac ctcgcccagg
     6541 cgcaggacga cgcgacgctt ctcgcgatcc gcgcgcagga ggacgccggg ctcgacatcg
     6601 tgaccgacgg cgagatccgg cgcgagagct attcgaaccg cttcgccacc gcgctcgagg
     6661 gcatcgacct cgacaacccg gggaccgcgc tcgaccgctc ggggcatccg aatcccgtgc
     6721 cgcgcatcgt cgggaagatc cgccggcgcc atcccgtgga gctcgaagac ctgaagttcc
     6781 tccgcgccca cacgcgccgg atgatcaaga tgacggtccc cggcccgttc accctgtcgc
     6841 agcaggcgca gaacgattac tacaaggatg ccgccgaggc ggcgatggac tacgccgccg
     6901 ccgtgaacga ggagatccgc gacctcttcg ccgccggcgc cgacatcgtc caggtcgacg
     6961 agccgtacat gcaggcgcgc ccggagaagg cgcgcgagta cgggctggcc gcgctcaacc
     7021 gcgcgctcga aggcatcacc gggacgacgg ccgtgcacat ctgcttcggc tacgcggcgg
     7081 tgatccatgt ccggccgtcg ggctactcgt tcctcggcga gctcgcgggc tgcacctgca
     7141 aacaagtgtc gatcgagacg gcgcagtcga acctcgactg cgcggtattg aagacgctcc
     7201 ccgggaagaa gatcatcctc ggcgtgatcg acctgaacga catgaaggtc gaaactccgg
     7261 agaccgtcgc cgcgcgcatc cgccgcgccc tgccctacgt caaggccgag gacgtcatcg
     7321 tcgcgcccga ctgcggcatg aaatacctgc cgcgcgacgt cgccttcggc aagatgacgg
     7381 cgatggccga aggcgcccgg atcgcccgcg ccgagctgac gcgctaggcg ttgcgccgca
     7441 gtctgacgaa cccggcttcc aggtcctcga gcagatcatc cgagctctcg agcccggcgt
     7501 ggacgcggaa gcacggcccg tgctcgggcc agcgcgtgac cgcccggcct tccatcgggt
     7561 gatacggcac gatcaggctc tcgtagccgc cccacgaggc gccgatgccg tagagcttca
     7621 tgccgtcgat cagcgcgaac atcgccttct ggtcgatgcc acgcttgagc accacgccga
     7681 agagacccga ggcgccggtg aagtcgcgct tccacagggc gtgcccggga tcggagggca
     7741 gcgcggggta cagcacgcgg tcgacctcgg gctgcttcgc gagccagccc gtgagcgccc
     7801 gcgcgttctc ggaatggatc ctcagccgcg ccgcgagcgt gcgcaggccg cgctggccga
     7861 ggtagaccac gtccggcccg gtggtctgcc ccatctcgta agtcgacgtc cgcagccggg
     7921 gccacgcctc cttcgtgcac gccaccgtcc cgagcatcgc gtccgagtgt ccgacgacgt
     7981 acttggtcgc cgcctgcacc gaaacgtcga cgccgtgcgc gagcgccttg aagtagagcg
     8041 gcgtcgccca ggtgttgtcc atgatcacga cgatgccgcg gcggtgcgcg acgtcggcga
     8101 tcgcgggcac gtcctgcacc tcgaaggtga gcgagcccgg cgactcgagg tagacgacgc
     8161 gcgtgttgcc ctggatcagc gactcgatgc cgcggccgac gagcgggtcg aagaaggtcg
     8221 tcgtcacgcc gaagcgcttg agcgtcccct tcgcgaagtt gcgcgtgggc ccataggtgc
     8281 tgtcgctcat cagcacgtga tcgcccgccg acaggaacgc ggtcagcgcc cccgcgcatg
     8341 ccgcgagccc cgacgggtag acgacggcgc ggtgcccgcc ctcgagctcg gcgatcgcct
     8401 cctgcagcgc ctcgacggtc ggcgtgccga agcggccgta gtaggtgccg ggcacctcgt
     8461 cgagccaggc gcggcgccgg gcgagatact cctcggccga gcgcgcgagg atggtcgagg
     8521 catggtacac gggcgggttc accgcgcctt cgtactgctc cggatggcgg ccggaatgga
     8581 cgatgacggt ctcgtcgcgc atggggctcc ttcccgggga ggaaatcgca aaggccctat
     8641 tctgaccctt aaatcgggcg gcaatctggc gccaatctga ccggggggct gccttagaat
     8701 aggccgcttt cccttcgctt cctcgtgact ctccccgtct cgcgcgcggt ccggtccatc
     8761 ggtcgcctcg cctggcccat cctggtcgcg cagctcgccg tcatggcgtc gggcgtcctc
     8821 gatacggtca tgtccgggca ttacagcgcg gtcgacctgg ccgcggtcgg catcggcgcg
     8881 agcatctact actcgatctt catcgggctg ctcggcgtgg tgcagtcgct gtcgccgatc
     8941 gccgcgcagc tgttcggcgc gaagcaatac gcgcgcatcg gcgaggaggc gcgccagacg
     9001 atgtggctcg ccaccggcct cgcgctctgc ggcgtgctgc tgctcgggtt tcccgagccg
     9061 ttcctgcggc tggccggcgc cccgcccgag gtcgaggcgc gcgtgcggct gtacctggtc
     9121 gggatcgcgt gggcgctccc cgggatgctg ctctttcgcg tgttcttcgc gttcacgacg
     9181 gcggtctcgc gtccgcgcgc ggcaatggcg ttgaatctct tctatttctg cgccaagctg
     9241 cccctcaatt gggtcttcat gtatggcaag ctcggcgccc cggagctcgg cggcccgggc
     9301 tgcgcggtcg cgaccgcggt ggtcgcgtgg gtcgcggcgc tcatcgggtg ggcgtattgc
     9361 gcccgcgatc cgttctaccg gccttttcgc gtgttcgcgc agtggtcttg gcccgaggcc
     9421 cgagtgctct ggagccatct caagctcggc gtgccgatgg gcctctcgtt cttcgtcgag
     9481 gtcacttcgt tcaccttcat cgcgatcttc gtcgcgcgcc tgggcgcgac gaccagcgcc
     9541 tcgcaccaga tcgcggccaa cgtctcggcg gtcgtctaca tgttcggcct cgcgatcggg
     9601 aacgcgacgg gcgtcctggt ggcgcaggcg gtgggcgccc gcagccgcca cgacgcgcgc
     9661 cgcaccgggc tcaccggcat ggcgatcatg atgctgatct cgggttgcgc gtccctggcg
     9721 ctcgtgcccg gcggggctgg gattgcgggc ctgtataccg acgatcccgg cgtgcggacc
     9781 ctcgccacgc ggctgctggc cttcgtcgcg ttctaccagc tcttcgacgg cgtgcaggtg
     9841 gtgatcgtca acgcgctgcg gggctaccgc atcgccttcc tgccgatgct ggtgtatacg
     9901 gtggcgctct gggggatcgg gctcgggggc ggctacgtgc tgggattgcg ggggtcggac
     9961 gcggcgcgct cgctcggcct ggcgacgccg atgggcgccg cggggttctg gctcgcgggc
    10021 gccttcggcc tgatcgtgac cggggcgatc ctgttcgcct atttcctgcg cgcgagcgcc
    10081 gtccgcccgg acgggcggac ggccgaagcg aacgtcggta cggcttaggc ggccttgttc
    10141 gccgacttcg ggaacagcgg ctgcgccgcg gccccagtgt cggtgatcgc gccgatgtcg
    10201 ccggagagct cgccgccctc ctcgatcgcg accttgccgt agcggatctt gcccgagacg
    10261 cgcccggtgg agcggatcac gagctgcttg cgcgccgtga gctcgccgtc gaagcggccg
    10321 cggatctcgg cgatgtcgat gccgaccttg cccacgaaca cgccgttctc ggcgatctgg
    10381 atcacgcggc tgtccatcga ggcctcgacg cggccttcga ccaccagggt gtcgcaatcg
    10441 gtgatttcgg cgcctttcag cttgatgtcg ggaccgacga tgagcttgct gacctgctcg
    10501 ggggctttct cgttttgcat ggttgcctgc gggttggttt cgggggcctt catttccggc
    10561 ttgttggccg gcacctggtc cgcgagctgg ctcggggtca aggcttcctg cagctcgccg
    10621 taggggcttt ggtaacgggc gacacgcgag ctgtctctcc tgcgaaaact atcgagcatt
    10681 tgctactccc tattgttcaa caaaggcgcc gggcggcggc gtccgcttcc ccccacgccg
    10741 gcgatgcgtg agcaagacgc gtgccgtacg aagcgaatgc gatttccata gggagaaagc
    10801 gagcattcgt cgctcgagcg tagcggaaat gtcacgcgcg cacgacatgc cgttcggatc
    10861 attcgatgta ataaactgtt tgcgctcgca aacggcgtgt cgcgcgcgag cgacaagcat
    10921 gcgcaggagc gtcacgccgc gagcgagaaa aactggagga gcgatcgatg cgaaatttga
    10981 ggacccgtga cgcgacgctg atcgcgctcg cgctgctcgc cgggtgcgtt gcgaccgaca
    11041 cgacgcgcac cccggcgctg gcgcggatcg aaaacgtcgt cgtgatctac gccgagaacc
    11101 gcagcttcga tcatctctac ggcctgtttc ccggcgccga gggcatcgcc gacgcgacgc
    11161 cggcgcagaa gacccagctc gacctcgacg ggcgcccgct gccgcacctg cccccgacct
    11221 acgagggcgg gaaggtgcgc gccgaccttc cgacgcagac gctcccgaac gctcccttcc
    11281 gcatcgacgc gccgccgatc ggccggcgct gggacgaggt gctaccgagc cccatccacg
    11341 cttatttcca gaaccgcga
//
LOCUS       DATDMW010000062         4082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_17041, whole genome shotgun sequence.
ACCESSION   DATDMW010000062 DATDMW010000000
VERSION     DATDMW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17041"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..807
                     /locus_tag="VI319_02635"
     CDS             37..807
                     /locus_tag="VI319_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY6862771.1"
                     /translation="MGTVAWISAASLGGGALSLALASVIAFSARASWIPALISFAIGA
                     LLGAVFLDILPHAFAAADQVQSVAATILAGILAFFVLEKLVLWRHCHVEDCEAHDRHA
                     APSDHGRSGLMITIGGGFHNFVDGVLIAAAFLESTQLGIVTAAAIIAHEIPQEIGDFV
                     ILLHSGYGKAAALTMNALSGLAMLAGALLGYLALHEVQAWIHTALALAAASMIYVAVA
                     DLIPGLHRRPELHATAQQVLLIALGVGAIWFVGDLTGA"
     gene            817..1818
                     /locus_tag="VI319_02640"
     CDS             817..1818
                     /locus_tag="VI319_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY6862772.1"
                     /translation="MRVLVTGAAGFIGHELCRRLLAEGEEVTGVDSLNDYYDVSLKRA
                     RLERLNGTRGFRFERLDLADRAAAAALFVRQGFDAVVHLAAQAGVRHSLVDPLAYVDA
                     NLAGFAHVLEGCRHARVGHLVFASSSSVYGANHRTPFSEHDPVDHPVSFYAATKRADE
                     LMAHVYAHLHGVPVTGLRFFTVYGPWGRPDMAYFSFTAKILAGTPLQIFNGGEMERDF
                     TYIDDIVEGVARVVRKPAVPEAGARSSPATSATAPFRIYNIGNNRPVKLMRFIEVLEH
                     CLGKKAVLEMCPMQPGDVIRTCADVRDLERAVGFRPDTPLDTGLARFVEWYRAYYRV"
     gene            complement(1823..2575)
                     /locus_tag="VI319_02645"
     CDS             complement(1823..2575)
                     /locus_tag="VI319_02645"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY6862773.1"
                     /translation="MKLAGRTAIVTGAARGIGKALAARLAADGAAVVIADIEGHEAAA
                     AALTASGARALGLKVDVSSESDTARMAERALEAFGRIDILVNNAAMFATVRIGAFEDI
                     PIAEWKRLLDVNVVGVALCCRAVTRHMRERGSGRIVNIASAAPLKGVPEFLHYVSSKG
                     AVIAMTRGLARELGRDGITVNAVAPGLTLSDATVGRAEHLRNHDASVRMRAIPRPQRP
                     DDLAGAVSFLASDDAAFMTGQTVVVDGGSAML"
     gene            2648..2863
                     /locus_tag="VI319_02650"
     CDS             2648..2863
                     /locus_tag="VI319_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY6862774.1"
                     /translation="MPRADGVYRVIEVIGTSTVSWEDAAKKAVQTASRTLRDLRIAEV
                     VKQDLKVDRGRVTAYRTRMQLSFKYER"
     gene            complement(2885..3310)
                     /gene="fur"
                     /locus_tag="VI319_02655"
     CDS             complement(2885..3310)
                     /gene="fur"
                     /locus_tag="VI319_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEY6862775.1"
                     /translation="MSNVHDLKTSGLKVTAPRTRILKLFESSKVRHLAAEDVYRQLMN
                     EGLDIGLATVYRVLTQFEQAGILQRHHFESGKAVFELNEGTHHDHLVCVQCGRVEEFY
                     DPEIEKRQVKVARDRGFSIREHSLHLYADCTKPHCPNKK"
     gene            3388..3741
                     /gene="bamE"
                     /locus_tag="VI319_02660"
     CDS             3388..3741
                     /gene="bamE"
                     /locus_tag="VI319_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY6862776.1"
                     /translation="MRITVLLLPLVLAGCGWLSPHRIEIQQGNYVTQDMVSQLKPGMT
                     RDQVRYVLGTPLVSDIFHADRWEYVFRRQRADSSKVEHGRLSVFFVDNKLERVDAEAV
                     PPPPLAGAQPDSVQR"
     gene            3738..>4082
                     /locus_tag="VI319_02665"
     CDS             3738..>4082
                     /locus_tag="VI319_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY6862777.1"
                     /translation="MKVAVAGAGGRMGRTLVELILGSGDLALSGALEAAGHPALGRDA
                     GSGVRITADAAAALKSAGCLIDFTTPEATLAHLEACVGAKVPMVIGTTGLSGPERKRV
                     AAAAERIAIVLAP"
BASE COUNT          581 a         1433 c         1401 g          667 t
ORIGIN      
        1 atggcggcct tgatctggat ggagcgctga gccgccatgg gaaccgtcgc gtggatctcg
       61 gccgccagcc tcggcggcgg cgcgctgagc ctggcgctcg cctcggtgat cgcgttctcg
      121 gcgcgcgcga gctggatccc ggcgctgatc agcttcgcca tcggcgcgct gctcggcgcg
      181 gtgttcctcg acatcctgcc gcatgcgttc gccgccgccg accaggtcca gtccgtcgcc
      241 gcgaccatcc tcgccggcat cctcgcgttc ttcgtgctcg agaagctcgt gctctggcgt
      301 cactgccacg tcgaggactg cgaggcgcac gaccggcacg cagcgccgag cgaccacggc
      361 cgcagcggcc tcatgatcac catcggcggc ggcttccaca acttcgtcga cggcgtgctc
      421 atcgccgccg cattcctcga gagcacgcag ctcgggatcg tgaccgcggc ggcgatcatc
      481 gcgcacgaga tcccgcagga gatcggcgac ttcgtcatcc tgctgcactc gggatacggc
      541 aaggcggcgg ccctgacgat gaacgcgctc tcgggcctcg cgatgctggc cggcgcgctg
      601 ctcggttacc tggcgctgca cgaggtgcag gcctggatcc acacggcgct cgcgctggcc
      661 gcggcgagca tgatctacgt ggccgtggcg gacctcatcc ccggactgca ccggcggccc
      721 gagctgcacg ccacggcgca gcaggtgctg ctcatcgccc tcggcgtcgg cgccatctgg
      781 ttcgtcggcg acctgaccgg ggcctagcgg tccggtatgc gcgtcctcgt caccggcgcc
      841 gcgggcttca tcgggcatga gctgtgccgg cggctgctcg ccgagggcga ggaagtgacg
      901 ggcgtcgaca gcctcaacga ctattacgac gtgtcgctga agcgcgcgcg ccttgagcgg
      961 ttgaacggga cgcgcggctt tcgtttcgag cggctcgatc tcgccgatcg cgcggctgcc
     1021 gcagcactct tcgtccgcca gggcttcgac gcagtcgtgc atcttgcggc gcaggccggc
     1081 gtgcggcatt cgctggtcga cccgctcgcg tacgtcgacg ccaacctcgc cgggttcgct
     1141 cacgtgctcg agggctgccg gcacgcccgc gtcggacacc tcgtgttcgc ctcgtcgagc
     1201 tcggtctacg gcgcgaacca tcgcacgccg ttctccgagc acgatcccgt cgatcacccg
     1261 gtgtcgttct atgcggcgac caagcgcgcc gacgagctga tggcgcacgt gtatgcgcac
     1321 ttgcacgggg tgcccgtcac gggtcttcgt ttcttcaccg tctacggccc gtgggggcgg
     1381 ccggacatgg cgtacttcag cttcaccgcg aagatccttg cggggactcc gctgcagatc
     1441 ttcaacggcg gggagatgga gcgcgacttc acctacatcg acgacatcgt cgaaggcgtt
     1501 gcccgcgtcg tccgcaagcc ggccgtcccg gaggccggcg cgcgctcgtc gcctgcgacg
     1561 agcgcgacgg cgcccttccg catctacaac atcggcaaca accgtcccgt caagctcatg
     1621 cgcttcatcg aggtgctcga gcattgcctc ggaaagaagg ccgtgctcga gatgtgcccg
     1681 atgcagcccg gcgacgtgat caggacctgc gccgacgtgc gcgatctcga gcgcgcggtg
     1741 ggattccggc cggatacgcc cctcgatacc gggctcgcgc gcttcgtgga gtggtaccgc
     1801 gcgtactacc gcgtctgagc gctcagagca tcgccgagcc gccgtcgacc acgacggtct
     1861 gtcccgtcat gaaggccgcg tcgtcgctcg cgaggaagct caccgcgccg gccaggtcgt
     1921 ccgggcgctg cgggcggggg atcgcccgca tgcgcacgct cgcgtcgtgg ttgcgcaggt
     1981 gctcggcgcg tcccacggtc gcgtccgaga gagtcagccc gggcgcgacc gcgttgaccg
     2041 tgatgccgtc gcggccgagc tcgcgcgcca gcccgcgcgt catcgcgatc accgcgccct
     2101 tgctcgagac atagtgaagg aattccggca cgcccttgag cggggccgcg gacgcgatgt
     2161 tgacgatgcg gccgctcccg cgctcccgca tgtgtcgggt caccgcccgg cagcacagcg
     2221 cgacgccgac cacgttgacg tcgagcagtc gcttccactc ggcgatggga atgtcctcga
     2281 acgcgccgat cctcaccgtc gcgaacatcg ccgcgttgtt gaccaggatg tcgatgcggc
     2341 cgaaggcttc gagggcccgc tcggccatgc gtgcggtgtc gctctccgac gagacgtcga
     2401 ctttcagccc gagagcgcgc gcgccgctcg ccgtcagtgc ggcggccgcg gcttcgtgtc
     2461 cctcgatgtc ggcgatcacg acggcggcgc cgtccgcggc gagccgcgcc gcgagcgcct
     2521 tgccgatgcc gcgcgcagcg ccggtcacga tcgcggtcct gcctgcgagc ttcatcggct
     2581 cctcctcggc gcgacgggtc gggagtatta tcgccgctcc cgcatatcgg tgcgacaagg
     2641 aggcgacatg ccgagggcag acggcgttta ccgggtgatc gaggtgatcg gaacgagcac
     2701 ggtctcgtgg gaggacgccg cgaagaaggc ggtgcagact gcgtcgcgca cgctgcgcga
     2761 cctgcgcatc gcggaggtcg tgaagcagga cctgaaggtc gaccgcggac gcgtcacggc
     2821 ctaccgcacg cggatgcagc tctcgttcaa atacgaaaga tgagacgaga gtagccggag
     2881 ggcgctattt cttgttcggg cagtgcggct tggtgcagtc cgcgtacagg tgcagcgagt
     2941 gctcgcggat ggagaagccc cggtccctgg cgaccttcac ctgccgcttc tcgatctccg
     3001 ggtcgtagaa ctcctcgacc cggccgcact gcacgcacac caggtggtcg tggtgcgtgc
     3061 cctcgttgag ctcgaacacg gccttgcccg attcgaaatg gtggcgctgc aggatccccg
     3121 cctgctcgaa ctgggtcagc acgcgataga cggtcgcgag gccgatgtcc aggccctcgt
     3181 tcatcagctg ccggtagacg tcctcggccg ccaagtgccg aaccttgctg ctctcgaaca
     3241 gcttgaggat gcgcgtcctg ggcgccgtga ccttgaggcc gctggttttc aggtcgtgga
     3301 cgttgctcat ggaagcctcc gcgcgggaag cgagcgggat tccgatatga tatagcgttt
     3361 tccttgcgcc tcaaaaaacg cgaacgcatg cgaatcaccg tcctgctcct gccgctcgtg
     3421 ctcgcgggct gcggctggct gtcgccgcac cgcatcgaga tccagcaggg caactacgtg
     3481 acgcaggaca tggtttcgca gctcaagccg ggcatgacgc gcgaccaggt gcgctacgtc
     3541 ctcggcacgc cgctcgtgag cgacatcttc cacgccgacc gctgggagta cgtgttccgg
     3601 cgccagcgcg ccgacagcag caaggtcgag cacggccgcc tctcggtctt cttcgtggac
     3661 aacaagctcg agcgcgtcga cgccgaggcc gttccgccgc cgccgctcgc gggcgcgcag
     3721 ccggacagcg tgcagcgatg aaggtcgccg tcgccggcgc gggcggccgc atgggccgga
     3781 cactggtcga gctgatcctc gggtcggggg atctcgcgct gtccggcgcg ctcgaggccg
     3841 cggggcaccc ggcgctcggc cgcgacgccg gatcgggcgt gcgcatcacg gcggacgcgg
     3901 cggccgcgct caagagcgcc ggatgcctga tcgacttcac gacccccgag gctacgctcg
     3961 cacacctcga agcctgcgtc ggcgcgaaag tccccatggt gatcggcacg acggggctct
     4021 cgggtcccga gcgcaagcgc gtcgccgcgg cggccgagcg gatcgcaatc gtgctcgcgc
     4081 cc
//
LOCUS       DATDMW010000063        15866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1760238, whole genome shotgun sequence.
ACCESSION   DATDMW010000063 DATDMW010000000
VERSION     DATDMW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15866
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760238"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VI319_02670"
     CDS             <1..1027
                     /locus_tag="VI319_02670"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEY6862778.1"
                     /translation="VNRSQSTNDVYPTAVNVALHFAIDALAAAMAGLRAAFGDKAREF
                     ATALKMGRTQLQDAVPMTLGQEFGTYAVMLEEDEQRLREAALLITEVNLGVTAIGTGI
                     NAHPDYAPLACEKLADITGIRVVTASNLVEATQDAGAFVQLSGVLKRIAVKLSKICND
                     LRLLASGPRAGLGEIVLPAVQAGSSIMPGKVNPVIPEVVNQIAFEVIGNDVTVSFAAE
                     AGQLQLNAFEPIIAHSLFKSIGHLARGIDTLRERCVTGIGANRERMAQMVERSIGIAT
                     ALNPHIGYAAATDVAREALATGKTVYELVLEKKLLPKERLDEILRPAALTRPGFVVPA
                     ATKKAEP"
     gene            complement(1024..3870)
                     /gene="uvrA"
                     /locus_tag="VI319_02675"
     CDS             complement(1024..3870)
                     /gene="uvrA"
                     /locus_tag="VI319_02675"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY6862779.1"
                     /translation="MEHIRIRGARTHNLKNLSVELPRNQLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKATSHNPRSTVGTIT
                     EVHDYLRLLYARVGTPFCPEHGIPLVAQSVSQMVDHVLALPEDTRLLILAPIVASRKG
                     EQAELFEDLRAQGFVRVRVDGTVHELDALPKLPKNVKHDVDVVIDRLKVRTDAKQRLA
                     ESFETALRHAEGRALAVETDSGKEHLFSAKFACPLDGYSLPELEPRLFSFNNPMGACP
                     ECDGLGTITFFDPKRVVALPELSLASGAIKGWDRRNQFYFQMLTSLARHFGFDVEAPF
                     QKLSEEAKNIVLYGTREKIPFSYLNERGKTFVKEHAFEGIIPNLERRHKETDSIVVRE
                     ELAKYLNTRACPACGGTRLRPEARNVKLGDAQDGKAIYEVSALPLKACLEFFRRLRLP
                     GAKQAIAEKIVNEIVARLSFLANVGLDYLSLDRSADTLSGGEAQRIRLASQIGSGLTG
                     VMYVLDEPSIGLHQRDNGRLLATLKHLRDLGNTVIVVEHDEEAILAADHVLDMGPGAG
                     EHGGQVVAEGTPEQIKRNPASLTGQYLSGKAEIPVPKQRRRVDKGAILRLRGARGNNL
                     KNVDLELPVGLLVCVTGVSGSGKSTLINDTLYQAVAHNLYGSAGEPAPHDSIKGLEFF
                     DKVINVDQSPIGRTPRSNPATDTALFTPIRELFAGVPTARERGYGPGRFSFNVKGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDVCHGRRYNRETLEVQYKGKNIHEVLHMNVEQAYE
                     FFSAVPAIERKLQTLLEVGLGYIQLGQSATTLSGGEAQRVKLSLELSKRDTGRTLYIL
                     DEPTTGLHFKDIDLLLQVLHRLRDHGNTVVVIEHNLDVVKTADWIVDLGPEGGDGGGR
                     IIATGTPEDIAAAPQSFTGQYLRHVLGKKVKRAA"
     gene            3964..5160
                     /locus_tag="VI319_02680"
     CDS             3964..5160
                     /locus_tag="VI319_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6862780.1"
                     /translation="MSPLELRASVSLASLFALRMLGLFLILPVFAVYALQLEGGQSHF
                     LVGVALGAYGLTQGLLQIPYGMASDRWGRKRVIVIGLLLFAAGSFVAAATSDINVVIL
                     GRALQGAGAISAPVTAFAADLTREQHRTKAMAMIGGSIGLMFALSLVAAPALYPVIGV
                     KGLFELTGVLSLAAVWVTLAVVPSEPGEHDRPHPNERRVEGSALGAVLRNREQLRLNL
                     GIFTLHVTQMAMFVVIPVAMVRHGGLPVSEHWKVYLPVVLGSFALMAPALLWAERRGR
                     MKPLFLGAIGLLALVELGFAAGYASLHATVLLLLAFFVAFNILEASLPSLVSRVAPAA
                     SRGTALGVYNTTQSLGLFVGAAVGGGLAGRFGEAAVFVFGTALVALWLGVAAGMRAPG
                     ETGARA"
     gene            5256..5720
                     /gene="ssb"
                     /locus_tag="VI319_02685"
     CDS             5256..5720
                     /gene="ssb"
                     /locus_tag="VI319_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY6862781.1"
                     /translation="MASVNKVILVGNLGRDPETRYNPEGGAICNISVATTDTWKDKTS
                     GEKQERTEWHRVVFFSRLAEIAGEYLKKGSQVYIEGSLRTRKWQDKEGQERYTTEIVA
                     DRMQMLGSRQGMGDAGAREKMGDAKEPVAAGAGPGAKKPGGKLDDLDDDIPF"
     gene            5804..6268
                     /gene="soxY"
                     /locus_tag="VI319_02690"
     CDS             5804..6268
                     /gene="soxY"
                     /locus_tag="VI319_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEY6862782.1"
                     /translation="MDRRAALKAGGGASVIALALAAGILKPEDVFAADWNKAAFETKS
                     FADTVKALGGGGSTESDQILMTTPDIAENGAVVPVTVESRLPGTRRIAILIEKNPNTL
                     SADFTLPEGTDPFVTTRVKMAETSNVYALVQSGGKFYHAAKEIKVTIGGCGG"
     gene            6270..6590
                     /gene="soxZ"
                     /locus_tag="VI319_02695"
     CDS             6270..6590
                     /gene="soxZ"
                     /locus_tag="VI319_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEY6862783.1"
                     /translation="MPDPMRIRANMAGDKVEVRVLMAHEMETGLRKDANGKLVPAHFI
                     QNVTVAHEGKTVLAAQWGTSISKNPFLHFRFKGGKPGDKVKITWVDNKGETRSDEATI
                     AAAG"
     gene            6592..7401
                     /gene="soxA"
                     /locus_tag="VI319_02700"
     CDS             6592..7401
                     /gene="soxA"
                     /locus_tag="VI319_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEY6862784.1"
                     /translation="MRALIMAAGAAALAVCAAAPAQDKDVTQREIERYRELLAEGNPA
                     DLAEAKGEELWKTMRGPKQASLERCDLGLGPGVVKGAYARLPRWFADTGRVQDLESRL
                     VTCMVTLQGFTEDEAKKNPFSAPGRPSPIEDIATYVAGASKGMTFDPPLRHPKELEAF
                     RTGEELFYRRAGPHDFSCATCHGDEGKRIRLQDLPKLTDPKEAQKVFTSWPAYRVSQG
                     SVRTMQHRLYDCYWQMRHPQLDYVSEASIDLQVFMAKYASGGTVEAPGLKR"
     gene            7398..8018
                     /gene="soxX"
                     /locus_tag="VI319_02705"
     CDS             7398..8018
                     /gene="soxX"
                     /locus_tag="VI319_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEY6862785.1"
                     /translation="MRSAILLAACAALLAGCTTPPPEDDARRQAQGAFERSFTHGNKE
                     MFARVVGQDDVQALCSRWRNRPPADVAERIRKSQAATLVYPADGKLMGDWRQGEKIAE
                     DGFGLRFTDTNQPKRPNGGNCYACHQLAPHEVSYGTLGPSLYRFGRTRGSSEAIQRYV
                     YGKVYNPEAFTACSGMPRFGLHQVLTPEQIRHVVALLLDPESPVNQ"
     gene            8035..9735
                     /gene="soxB"
                     /locus_tag="VI319_02710"
     CDS             8035..9735
                     /gene="soxB"
                     /locus_tag="VI319_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HEY6862786.1"
                     /translation="MSRREFLQALATAAAGGFVLDARGEDAAAQYDVPRFGNVSLLHF
                     TDTHAQLLPVYFREPSVSIGVGEGANRPPHLVGEALLRAFGVRPGTREAHAFTSLDFE
                     RAARRYGRMGGFAHLATLVARLRATRPGALLLDGGDSWQGSAVSLWTQGRDMIAAQKR
                     LGVDVMTGHWEFTYGAARVREAVKNELGRIAFLAQNVETADFGDPVFEPWTMREVSGV
                     PVAIVGQAFPYVPIAHPRRFVEQWSFGIQEARLQKHVDAARAKGAQAVVLLSHNGMDV
                     DLKLAARVTGIDVILGGHTHDAVPAPVAVGNARGKTLVTNAGSNGKFLAVLDLDVRGG
                     KVADARYRLLPVFASLLPADPGMAALIEQTRAPWRERLVEPLATTEGLLYRRGNFNGT
                     TDQLIVDALLETKDAEIAFSPGFRWGPALLPGETITMERLLGQTAITYPQVTLSEMTG
                     ETLKSVLEDICDNLFNPDPYYQQGGDMVRVGGMSYTCRPTAAMGARIVDMRVRGKPVE
                     PARRYKVASWAPVGDAPPDAEPVWDVVARWLRARKSVAPRVLNRPRLEGVAGNRGLAS
                     "
     gene            9813..10142
                     /locus_tag="VI319_02715"
     CDS             9813..10142
                     /locus_tag="VI319_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY6862787.1"
                     /translation="MPIYEYRCSSCGFQKEYLQKVTDPILTRCPECDKDTFSKLLSAA
                     GFQLKGSGWYATDFKNSGAKTAAPASGGKTESKDSGKSESKDGGKSKETKSEAVSCGA
                     GACPSCS"
     gene            10183..10791
                     /locus_tag="VI319_02720"
     CDS             10183..10791
                     /locus_tag="VI319_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY6862788.1"
                     /translation="MKKYFITGLLIWIPLAITIWVLQLIVTTMDDVLPGQFQLALFGR
                     QVPGLGVLLTLLIVLGTGVLASNILGQRLVLLWEMLLGRIPVVKTIYSSVKQVSDTLF
                     SPGGQAFRKALLVQYPHPGSWTIAFLTGAPGGDVPNHLKGDYVSVYVPTTPNPTSGFF
                     LMFARTDVIELDMSVDEALKYVISMGVVAPARTPPRNPEALH"
     gene            10788..11411
                     /locus_tag="VI319_02725"
     CDS             10788..11411
                     /locus_tag="VI319_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862789.1"
                     /translation="MTAPAAAVRSGLVIALLALYSVASHLAATSGVAGYAWLAWSSLV
                     CGVALALPWKAGLALALALIGPMPWIPIAWLLAIPPVVIYLALAVWFGRTLLPGRVPL
                     ISRLASLERGDLEPVLARYTRRLTAIWTAFFVAMATLAAVLALLGDGHTWSLFTNGVS
                     YLLMGALFFGEYAYRRLRYSEFRHASLPRMIRMLVAAGRTARRAQGR"
     gene            11485..12279
                     /locus_tag="VI319_02730"
     CDS             11485..12279
                     /locus_tag="VI319_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6862790.1"
                     /translation="MTSSQEIEFAVVIPAYNEAATIRGVVTRTLRYAGRVIVVDDGSI
                     DGTSAALEGLPVTLLAHERNQGKAASLWRGMAHALSLGVDAVVTLDADGQHDPADIPR
                     LVSAARRSPGSIVIGARLADKVKIPPLRYFANRFANFWIAWAAGYPIEDSQSGFRVYP
                     VSLLQRVRVPHGPTKGFVFESEILIAASRLGYESVPVRVAAIYPPEGRPSHFRQFVDV
                     ERIVRMVTLKLLARGLHPLGLLRSLRRRPPLLDDEDAEERASAYKS"
     gene            12311..14089
                     /gene="aspS"
                     /locus_tag="VI319_02735"
     CDS             12311..14089
                     /gene="aspS"
                     /locus_tag="VI319_02735"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY6862791.1"
                     /translation="MRTHYCGKVDAALLGKTVTLYGWAHRRRDHGGVIFIDLRDREGL
                     LQVVSDPDRRETFATAEKIRSEYVLKVTGVVRRRPEGTVNPNLRSGEVEVLAHEIEIL
                     NASLTPPFQIDDDNLSENVRLTYRVLDLRREAMQKNLRLRYRLAMAVRRYLDEQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQMLMVAGYDRYYQIVKCFR
                     DEDLRADRQPEFTQIDCETSFLGEAEIRALFEGMIRGVFREAIGVSLPEPFPVMTYAE
                     AMRDYASDKPDLRIPLKLTELTDAMKSVEFKVFSGPASTPGHRVAGLLVPGGGALTRG
                     ELDAYGEYVAQFGAKGLAWIKFNEVKKGREGMQSPIVKNLSDAALATIVERSGAKDGD
                     LMFFGAGRNKVVNDALGALRVKLGHDRGLVQAGWRVLWVVDFPMFEYDEEEKRWNAMH
                     HPFTAPKDGHEEKLATDPGAALAKAYDMVLNGSEIGGGSVRIHREEVQSKVFRAMSIS
                     HDEARNKFGFLLDALQYGAPPHGGIAFGLDRLAALMAGCESIRDVIAFPKTQRAQDLL
                     VKAPSAVDEKQLRELHIRLRKSEEPK"
     gene            14095..14508
                     /locus_tag="VI319_02740"
     CDS             14095..14508
                     /locus_tag="VI319_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEY6862792.1"
                     /translation="MADAAQAGVFHLGPDARAAAQAAASAGLLVAYVDIGHAHDKEHF
                     AEHVAEALRFPGGHDTDWDTFVKGVTDLSWLEAKGWVVILEKSKHFCGGHRHEFEDAM
                     RVMSDAAAHWRARGRPFWTLIGGPEGWKSGWPDLP"
     gene            14505..14966
                     /gene="nudB"
                     /locus_tag="VI319_02745"
     CDS             14505..14966
                     /gene="nudB"
                     /locus_tag="VI319_02745"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEY6862793.1"
                     /translation="MSYKIPRSALVVIHTPDLQVLMMERTSWPGFWQSVTGGLDREDE
                     PYRDAAVREVREETGIDATKYALTDWGLEREFEIFARHRHRYAPGVARNLEHVFSLEV
                     PAPLEVRLEPAEHLRYRWLPYREAAALTISWSNRDAILALPERRAQVSRKP"
     gene            15006..15575
                     /locus_tag="VI319_02750"
     CDS             15006..15575
                     /locus_tag="VI319_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY6862794.1"
                     /translation="MAAPRLAALLALVVCVAPCAPAAAQAIALETYRQGDAVNVYAEV
                     RLDVDPKIAWDVLSDYDHLAEFVPDMSYSRVLSRDGNKVTIEQKGEFGVLFFRQPIEL
                     KLEIVETPHRTIVARAVGGSFREMTGRYDLDRVEGGGVRIGYAGHFVPAFPLPTFFGI
                     IGVRHTAASQLSALVDEIVRRDARARSGH"
     gene            15664..>15866
                     /locus_tag="VI319_02755"
     CDS             15664..>15866
                     /locus_tag="VI319_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EEP domain-containing protein"
                     /protein_id="HEY6862795.1"
                     /translation="MNLHIATYNIHKGFTHFNRRMMVHELRDQLRSLGADIVFLQEVQ
                     GMHHRHASRFENWPIEPQYEFLA"
BASE COUNT         2424 a         5484 c         5552 g         2406 t
ORIGIN      
        1 cgtcaacagg agccagagca cgaacgacgt ctatcccacg gcggtgaacg tcgcgctgca
       61 tttcgcgatc gacgcgctcg ccgccgccat ggcgggcctg cgcgccgcgt tcggcgacaa
      121 ggcgcgcgag ttcgccaccg cgctcaagat gggccgcacg cagctgcagg acgcggtgcc
      181 gatgacgctc gggcaggagt tcggcaccta tgccgtcatg ctcgaggagg acgagcagcg
      241 gctgcgcgag gcggcgctgc tcatcaccga ggtgaacctc ggcgtgacgg cgatcggcac
      301 cggcatcaac gcgcaccccg actacgcgcc gctcgcgtgc gagaagctcg ccgacatcac
      361 cggcatccgc gtggtcacgg cgtcgaacct ggtcgaggcg acacaggacg cgggcgcctt
      421 cgtacagctc tccggcgtgc tgaagcgcat cgcggtcaag ctctcgaaga tctgcaacga
      481 cctgcggctg ctcgcctcgg gcccgcgcgc ggggctgggc gagatcgtcc tgcccgcggt
      541 ccaggcgggc tcctccatca tgccggggaa ggtgaacccg gtgatccccg aggtcgtgaa
      601 ccagatcgcc ttcgaggtga tcgggaacga cgtgacggtc agcttcgccg ccgaggccgg
      661 ccagctgcag ctcaacgcct tcgagccgat catcgcgcac agcctgttca agagcatcgg
      721 ccacctcgcg cgcggcatcg acacgttgcg cgagcgctgc gtcacgggga tcggcgcgaa
      781 ccgggagcgc atggcgcaga tggtggagcg gtcgatcggc atcgccacgg cgctcaatcc
      841 ccacatcggt tacgccgccg cgaccgacgt ggcgcgcgag gcgctggcga ccgggaaaac
      901 ggtgtacgag ctcgtgctcg agaagaagct gctcccgaag gagcgcctcg acgagatcct
      961 gagacccgcg gcgctgacgc ggccgggctt cgtcgtgccg gcggcgacga agaaggcgga
     1021 gccctaggcc gcgcgcttca ccttcttgcc gaggacgtgt cgcaggtact gcccggtgaa
     1081 gctctgcggc gcggcggcga tgtcctcggg cgtgccggtg gcgatgatgc gcccgccgcc
     1141 gtcgccgccc tcgggaccga gatcgacgat ccagtcggcg gtcttcacga cgtcgagatt
     1201 gtgctcgatg acgacgacgg tgttgccgtg gtcgcgcagg cggtgcagca cctgcagcag
     1261 gagatcgatg tccttgaagt gcaggccggt cgtcggctcg tcgaggatgt agagcgtgcg
     1321 cccggtgtcg cgcttggaga gctccagcga cagcttcacg cgctgcgcct ccccgcccga
     1381 gagcgtcgtc gccgactggc cgagctggat gtagccgagg ccgacctcga gcagggtctg
     1441 gagcttgcgt tcgatggccg gcacggcgct gaagaactcg taggcctgct cgacgttcat
     1501 gtgcaggacc tcgtggatgt tcttcccctt gtactgtacc tcgagcgtct cgcggttgta
     1561 gcgccgtccg tggcagacgt cgcacggcac gtagatgtcg ggcaggaagt gcatctcgac
     1621 cttgatcacg ccgtcgccct ggcaggcctc gcagcggccg cccttgacgt tgaacgagaa
     1681 gcgccccggg ccgtagccgc gctcgcgcgc cgtcggcacg cccgcgaaca gctcgcggat
     1741 cggcgtgaac agcgccgtat ccgtcgccgg attggagcgc ggcgtgcgcc cgatcgggct
     1801 ctggtcgacg ttgatcacct tgtcgaagaa ctcgaggccc ttgatcgaat cgtgaggcgc
     1861 gggctcgccc gccgagccgt acaggttgtg cgcgaccgcc tggtagagcg tgtcgttgat
     1921 cagcgtcgac ttgcccgatc ccgacacgcc ggtcacgcac acgagcagac cgacgggaag
     1981 ctcgaggtcg acgttcttga ggttgttgcc gcgcgcgccg cgcagccgca ggatcgcgcc
     2041 cttgtcgacg cgccggcgct gcttcggcac ggggatctcg gccttgcccg agagatattg
     2101 cccggtgagc gaggcggggt tgcgcttgat ctgttccggg gtcccctcgg cgaccacctg
     2161 gccgccgtgc tcgccggcgc cgggccccat gtcgagcacg tggtcggcgg cgaggatcgc
     2221 ctcctcgtcg tgctcgacca cgatcaccgt gttgccgaga tcgcgcaggt gcttgagcgt
     2281 cgcgaggagc cgcccgttgt cgcgctggtg cagcccgatc gacggctcgt cgagcacgta
     2341 catgaccccg gtcaggcccg agccgatctg cgaggcgagc cggatgcgct gcgcctcgcc
     2401 gcccgagagc gtgtcggcgg agcggtcgag cgagaggtag tccaggccga cgttcgcgag
     2461 gaacgagagg cgcgcgacga tctcgttgac gatcttctcg gcgatggcct gcttcgcgcc
     2521 gggaagccgc agcctgcgga agaactccag gcacgccttc aacggcagcg cgctcacctc
     2581 gtagatggcc ttgccgtcct gggcgtcgcc gagcttgacg ttgcgcgcct cgggacgcag
     2641 gcgcgtgccg ccgcacgcgg gacaggccct ggtgttgaga tacttggcga gctcctcgcg
     2701 cacgacgatc gagtcggtct ccttgtggcg ccgctcgagg ttggggatga tgccctcgaa
     2761 ggcgtgctcc ttcacgaagg tcttgccgcg ctcgttcagg taggagaacg ggatcttctc
     2821 ccgcgtgccg tagaggacga tgttcttcgc ctcctcggac agcttctgga acggtgcctc
     2881 gacgtcgaag ccgaagtggc gcgcgaggct ggtcagcatc tggaagtaga actggttgcg
     2941 gcggtcccag cccttgatcg cgccgctcgc gagcgacagc tcgggcagcg ccacgacgcg
     3001 cttcgggtcg aagaaggtga tcgtgcccag cccgtcgcac tcggggcagg cgcccatcgg
     3061 gttgttgaac gagaacaggc gcggctcgag ctcgggcagc gagtagccgt cgagcgggca
     3121 ggcgaacttc gccgaaaaga gatgctcctt gccgctgtcc gtttccaccg cgagcgcgcg
     3181 cccctcggca tggcgcagcg cggtctcgaa cgactcggcg aggcgctgct tagcgtcggt
     3241 ccggaccttg agccggtcga tcaccacgtc gacgtcgtgc ttcacgttct tggggagctt
     3301 gggcagcgcg tcgagctcgt gcaccgtgcc gtcgacgcgc acgcggacga acccttgagc
     3361 gcgcaggtcc tcgaacagct cggcctgctc gcccttgcgg ctcgcgacga tgggcgcgag
     3421 gatcaggagc cgggtgtcct cgggaagggc gaggacatgg tcgaccatct gcgacacgga
     3481 ctgcgcgacc agcgggatcc cgtgctcggg gcagaacggc gtgccgacgc gcgcgtacag
     3541 cagccgcagg taatcgtgca cctccgtgat ggtgccgacg gtggagcgcg gattgtggct
     3601 ggtcgccttc tgctcgatcg agatcgcggg cgagagcccc tcgatcaggt cgacgtcggg
     3661 cttctccatc agctgcagga actggcgcgc gtacgccgag agcgactcga cgtagcgtcg
     3721 ctggccttcc gcgtacagcg tgtcgaaggc gagcgaggac ttgcccgagc ccgagaggcc
     3781 ggtgatgacg atcagctggt tccgcggcag ctcgacgctg aggttcttca ggttgtgcgt
     3841 gcgcgcgccg cggatgcgga tgtgctccat tccagagggg tgcaaagtca aacctgctag
     3901 tatacgaaat gtcgcagtgt ccccgctact aaaatgagca acggtccggt ggcggccgcc
     3961 cggatgtccc ccctcgaatt gcgcgcgagc gtgtcgctcg cgtcgctgtt cgcgctgcga
     4021 atgctcggat tgtttctcat cctccccgtg ttcgcggtgt acgcgctgca attggagggc
     4081 gggcagagcc acttcctggt cggggtggcg ctcggcgcct acggcctgac gcaagggctg
     4141 ctgcagatcc cgtacggcat ggcgtccgat cgctggggcc gcaagcgcgt catcgtgatc
     4201 gggctgctgc tgttcgcggc cggcagcttt gtcgctgccg cgacttcaga cataaacgtc
     4261 gtcatcctgg gccgcgcgct ccagggcgcg ggcgcgatct cggcgccggt caccgcgttc
     4321 gccgccgacc tgacgcgcga gcagcaccgc accaaggcga tggcgatgat cggcggctcg
     4381 atcggcctga tgttcgcgct gtcgctggtc gcggcgccgg cgctctatcc ggtgatcggg
     4441 gtgaagggtc tgttcgagct gacgggggtg ctttcgctcg ccgccgtgtg ggtcacgctc
     4501 gcggtcgtgc cctccgagcc cggcgagcat gatcgcccgc acccgaatga gcgacgcgtc
     4561 gaaggcagcg ccctcggcgc ggtgctgcgc aaccgcgagc agctgcggct caacctcggc
     4621 atcttcacgc tgcacgtcac gcagatggcg atgttcgtgg tgatcccggt cgcgatggtc
     4681 cgccacggcg gcctcccggt cagcgagcac tggaaggtgt acctgccggt ggtgctcggc
     4741 tcgttcgcgc tcatggcgcc cgcgctgctc tgggcggagc ggcgcgggcg catgaagccg
     4801 ctgttcctcg gtgccatcgg cctgctcgcg ctggtcgagc tcggcttcgc tgccgggtac
     4861 gcgagcctgc acgcgacggt gctgctgctg ctcgcgttct tcgtggcttt caacatcctc
     4921 gaggcgagcc tgccgtcgct cgtgtcgcgg gttgcgccgg ccgcgtcccg gggcacggcg
     4981 ctgggcgtct acaacacgac ccagtcgctc gggctgttcg tcggcgcggc cgtgggcggg
     5041 ggcctcgccg gacgtttcgg cgaggccgcg gtctttgtat tcggaacggc gctcgttgcg
     5101 ttatggctgg gagtggcggc gggaatgcgc gcgccgggcg agacgggcgc tcgagcgtag
     5161 acctggtccg tccggcgcag gcgctcgcgc ctcgggcggg gcgcggcatg gtcaacggtg
     5221 tatcctgata tcttcggacg cgagggagac gactcatggc atcggtcaac aaggtgatcc
     5281 tggtgggcaa tctgggccgc gacccggaga cccgctacaa ccccgagggc ggggcgatct
     5341 gcaacatctc ggtggcgacc accgacacgt ggaaggacaa gacgagcggc gagaagcagg
     5401 agcgcaccga gtggcaccgc gtggtgttct tcagccggct cgcggagatc gccggcgagt
     5461 acttgaagaa gggctcgcag gtctacatcg aaggcagcct gcgcacgcgc aagtggcagg
     5521 acaaggaagg ccaggagcgc tataccaccg agatcgtcgc cgaccgcatg cagatgctcg
     5581 gcagccgcca gggcatgggc gacgccggcg cgcgcgagaa gatgggcgac gccaaggagc
     5641 ccgtcgccgc gggcgcgggg ccgggcgcga agaagccggg cggcaagctc gacgacctgg
     5701 acgacgacat cccgttctag gcgcgaccgc ggcccgcgcc ggagcgcgcg cgggcacgat
     5761 gctaaaatgc gcgctcgata tcgcgcattt tcccggagca tcgatggaca gaagagcagc
     5821 actcaaggcc ggcggcggcg cgagcgtgat cgcgctcgcg ctcgccgcag ggatcctgaa
     5881 acccgaggac gtgttcgccg cggactggaa caaggccgcg ttcgagacca agtccttcgc
     5941 cgacacggtg aaggcgctcg gcggcggcgg gtcgaccgag agcgaccaga tcctgatgac
     6001 gacgcccgac atcgccgaga acggcgcggt cgtgccggtc acggtcgaga gcaggctgcc
     6061 cggcacgcgc cgcatcgcga tcctgatcga gaagaacccg aacacgctct ccgccgactt
     6121 caccctcccg gaagggacgg atccgttcgt caccacgcgc gtgaagatgg ccgagacctc
     6181 gaacgtgtac gccctggtcc agtcgggcgg gaagttctat cacgccgcga aggagatcaa
     6241 ggtgaccatc ggcgggtgcg gaggctgata tgcccgatcc gatgcgcatc cgcgccaaca
     6301 tggccggcga caaggtggaa gtgcgcgtgc tgatggcgca cgagatggag acgggcctgc
     6361 gcaaggacgc gaacgggaag ctcgtgccgg cgcacttcat ccagaacgtc accgtggcgc
     6421 acgagggaaa gaccgtgctc gcggcgcagt ggggcacctc gatctcgaag aacccgttcc
     6481 tgcacttccg cttcaagggc ggcaagcccg gcgacaaggt gaagatcacc tgggtcgaca
     6541 acaagggcga gacgcgcagc gacgaggcga ccatcgccgc ggcgggctga gatgcgcgcg
     6601 ctgatcatgg ccgccggcgc ggctgcgctc gccgtctgcg ccgccgctcc ggcgcaggac
     6661 aaggacgtca cgcagcgcga gatcgagcgc taccgcgagc tgctcgccga gggcaacccg
     6721 gccgatctcg ccgaggcgaa gggcgaggag ctgtggaaga cgatgcgcgg cccgaagcag
     6781 gcgtcgctcg agcgctgcga cctcgggctc gggccgggcg tcgtcaaagg cgcgtacgcg
     6841 cgcctgccgc gctggttcgc cgacaccggc cgcgtgcagg acctcgagtc gcgcctggtg
     6901 acctgcatgg tgacgctgca gggattcacc gaggacgagg cgaagaagaa cccgttctcc
     6961 gcgcccgggc gtccttcgcc gatcgaggac atcgcgacct acgtcgccgg cgcgtcgaag
     7021 ggcatgacgt tcgatccgcc gctccgccat ccgaaggagc tcgaggcgtt ccgcaccggc
     7081 gaggaattgt tctaccgccg cgccggtccg cacgatttct cgtgcgccac ctgccacggc
     7141 gacgagggca agcgcatccg cctgcaggac ctacccaagc tcaccgatcc gaaggaggcg
     7201 cagaaggtgt tcaccagctg gcccgcgtac cgcgtgtcgc agggatcggt gcgcaccatg
     7261 cagcaccgcc tgtacgactg ctactggcag atgcgccatc cgcagctcga ctacgtctcc
     7321 gaggcgtcga tcgacctgca ggtgttcatg gcgaagtacg cgagcggcgg cacggtggaa
     7381 gcgccgggcc tgaagcgatg agaagcgcga tcctgctcgc ggcctgcgcc gcgctcctcg
     7441 ccggctgcac cacgccgccg ccggaggacg acgcgcggcg ccaggcgcaa ggcgcgttcg
     7501 agcgcagctt cacgcacggc aacaaggaga tgttcgcgcg cgtcgtcggc caggacgacg
     7561 tgcaggcgct gtgcagccgc tggcgcaacc ggccgccggc cgatgtcgcc gagcgcatcc
     7621 ggaagtcgca ggccgcgacc ctcgtctacc ccgcggacgg gaagctgatg ggcgactggc
     7681 gccagggcga gaagatcgcc gaggacggct tcggcctgcg cttcaccgac acgaaccagc
     7741 cgaagcggcc gaacggcggc aactgctacg cctgccacca gctcgcgccg cacgaagtct
     7801 cgtacgggac gctcgggccc agcctctacc gcttcggccg cacgcgcgga tcgagcgagg
     7861 cgatccagcg ctacgtctac ggcaaggtct acaaccccga ggccttcacc gcctgctcgg
     7921 gcatgccgcg cttcgggctt caccaggtgc tgacgcccga gcagatccgg cacgtcgtcg
     7981 cgctgctgct cgaccccgag tctcccgtca accagtagcg ccacgcgcgt gtcgctgtcc
     8041 cggcgcgaat tcctgcaggc gctcgcgacc gcggccgcgg gcggctttgt cctggatgcg
     8101 cgcggggagg acgcggcggc gcagtacgac gtgccgcggt tcggcaacgt gtcgctgctc
     8161 cacttcaccg acacgcacgc gcagctgctc ccggtatatt ttcgcgagcc gagcgtcagc
     8221 atcggcgtcg gcgaaggcgc gaaccgcccg ccgcacctcg tgggcgaggc gctgctgcgc
     8281 gcgttcggcg tccgccccgg gacgcgcgag gcgcacgcct tcacctcgct cgacttcgag
     8341 cgcgccgcgc gccgctacgg ccgcatgggc ggcttcgcgc acctcgcgac gctggtcgcg
     8401 cgcctgcggg cgacgcgccc cggcgcgctg ctgctcgacg gcggcgacag ctggcagggc
     8461 tcggccgtgt cgctgtggac gcaaggccgc gacatgatcg cggcgcagaa gcgcctcggc
     8521 gtcgacgtca tgaccgggca ctgggagttc acctacggcg cggcgcgcgt gcgcgaggcg
     8581 gtgaagaacg agctcggccg catcgccttc ctcgcgcaga acgtcgagac cgccgacttc
     8641 ggcgatccgg tgttcgagcc atggacgatg cgcgaggtga gcggcgtgcc ggtcgcgatc
     8701 gtcggccagg cgttccccta cgtgccgatc gcccacccgc ggcgcttcgt cgagcagtgg
     8761 agcttcggca tccaggaggc gcgcctgcag aagcacgtcg acgccgcgcg cgcgaagggc
     8821 gcgcaggccg tggtgctgct ctcccacaac ggcatggacg tcgacttgaa gctcgccgcg
     8881 cgcgtcaccg ggatcgacgt gattctcggc ggccataccc acgacgcggt gcccgcgccg
     8941 gtcgccgtcg gcaacgcgcg cggcaagacg ctcgtgacca acgccggctc gaacggcaag
     9001 ttcctcgccg tgctcgacct cgacgtgcgc ggcggcaagg tggcggacgc gcgctaccgg
     9061 ctgctgcccg tgttcgcgtc gctgctgccc gccgatcccg gcatggcggc gctgatcgag
     9121 cagacgcgcg cgccgtggcg cgagcgcctc gtcgagccgc tcgccacgac cgagggcctg
     9181 ctctaccgcc gcggcaactt caacggcacg accgaccagc tgatcgtcga cgcgctgctc
     9241 gagacgaaag acgcggaaat cgccttctcg cccgggttcc gctgggggcc ggcgctcctg
     9301 cccggcgaga ccatcacgat ggagcggctg ctcggccaga ccgcgatcac gtacccgcag
     9361 gtgacgctgt ccgagatgac cggcgagacg ctgaagagcg tgctcgagga catctgcgac
     9421 aacctcttca accccgatcc ctactatcag cagggcggcg acatggtgcg ggtcggcggc
     9481 atgagctata cctgccggcc gaccgccgcc atgggagccc ggatcgtcga catgcgcgtg
     9541 cgcggcaagc cagtcgagcc ggcccggcgt tacaaggtcg cgagctgggc gccggtcggc
     9601 gacgcgccgc cggacgcgga gcccgtgtgg gacgtggtcg cgcgctggct gcgggccagg
     9661 aagagcgtgg cgccgcgcgt gctgaaccgc ccgcggctcg aaggggtcgc cggaaatcgc
     9721 ggcctcgcct cttgaaaccg gcgccgcgcg cccctatttc ccgagggaat tcaacaggtc
     9781 cggggtataa tcccggcttt gcggagagcg ccatgccgat ctacgaatat cgctgctcct
     9841 cctgcgggtt ccagaaggag tacctgcaga aggtgaccga cccgatcctg acccgctgcc
     9901 ccgagtgcga caaggacacc ttctcgaagc tgctgtcggc ggccggattc cagctcaaag
     9961 gcagcggctg gtacgcgacc gacttcaaga acagcggcgc gaagaccgcc gcgcccgcgt
    10021 ccgggggcaa gaccgaatcc aaggacagcg gcaagtcgga gtccaaggac ggcggcaagt
    10081 ccaaggagac gaagtcggaa gccgtgtcct gcggcgcggg cgcctgcccc agctgctcct
    10141 gaagtttccc gcccgcactc ccgcggcggc ccgggccgca acttgaaaaa atatttcatc
    10201 accgggctcc tgatctggat cccgctcgcg atcacgatct gggtgctgca gctcatcgtg
    10261 acgacgatgg acgacgtgct gccggggcaa ttccagctcg cgctgttcgg gcggcaggtc
    10321 ccgggcctcg gcgtcctgct caccctgctc atcgtgctcg gcaccggcgt gctcgcctcg
    10381 aacatcctcg gccagcggct ggtcctgctc tgggagatgc tgctcgggcg catcccggtc
    10441 gtgaagacga tctactcgag cgtcaagcag gtgagcgaca cgctgttctc gccgggcggc
    10501 caggcgttcc gcaaggcgct gctggtgcag tatccgcacc cgggctcgtg gaccatcgcc
    10561 ttcctcaccg gcgcccccgg cggcgacgtg cccaaccatc tgaagggcga ttacgtgagc
    10621 gtgtacgtcc cgacgacgcc caacccgacc tcggggttct tcctgatgtt cgcgcggacc
    10681 gacgtgatcg agctcgacat gagcgtcgac gaggcgctga agtacgtgat ctcgatgggc
    10741 gtggtcgcgc ccgcgcggac cccgccgcgc aatccggaag cgctgcattg accgcacccg
    10801 cagccgccgt gcgcagcggg ctggtgatcg cgctgctcgc cctctacagc gtcgcatcgc
    10861 atctcgccgc caccagcggc gtcgcgggct acgcctggct cgcatggtct tcgctcgtct
    10921 gcggcgtcgc gctcgcgctg ccctggaagg ccgggctcgc gctcgcgctc gcgctgatcg
    10981 gcccgatgcc gtggatcccc atcgcctggc tgctcgcgat tccgcccgtc gtgatctatc
    11041 tcgcgctcgc ggtgtggttc ggccggacgc tgctccccgg gcgcgtgccg ctcatcagcc
    11101 gcctggcgag cctcgagcgc ggggatctcg agcccgtcct cgcccgctac acccgccgcc
    11161 tgaccgcgat ctggaccgcg ttcttcgtcg cgatggcgac gctcgcggca gttctcgcgc
    11221 tgctcgggga cggccacacc tggtcgctct tcacgaacgg cgtgagctat ctcctcatgg
    11281 gggcgctgtt cttcggcgag tacgcgtacc ggcgcctgcg ctacagcgag ttccgccacg
    11341 cgtcgctgcc gcgcatgatc cgcatgctgg tggcggcagg caggacggcc cgccgggcgc
    11401 aaggccggtg aatcgccgat tttacgaatt cgcgtttgtt atactgcggc tggttcaatc
    11461 agccccaagc ggttcgccgg gatcgtgacg tcctcccaag agatcgaatt cgccgtcgtc
    11521 attccggcgt acaacgaagc cgccaccatc cgcggggtgg tgacgcggac gctgcgctac
    11581 gccgggcgcg tgatcgtcgt cgacgacggc tcgatcgacg ggacctcggc ggcgctcgag
    11641 ggcctgcccg tgacgctgct cgcgcacgag cgcaaccagg gcaaggcggc gagcctctgg
    11701 cgaggcatgg cgcacgccct ctcgctcggc gtcgatgcgg tggtcacgct cgacgccgac
    11761 ggccagcacg accccgcgga catcccgcgc ctcgtgagcg cggcgcggcg ctcgccgggc
    11821 agcatcgtga tcggcgcgcg gctcgccgac aaggtcaaga tcccgccgct gcgctacttc
    11881 gccaaccgct tcgcgaactt ctggatcgcg tgggccgcgg gctatccgat cgaggacagc
    11941 cagtcgggct tccgcgtcta tcccgtgtcg ctgctccagc gcgtgcgcgt gccgcacggc
    12001 cccaccaagg gcttcgtctt cgagagcgag atcctgatcg cggcgagccg gctcggctac
    12061 gagagcgtcc cggtccgcgt ggcggccatc tatcccccgg aaggacggcc gagccatttc
    12121 cggcagttcg tcgacgtcga gcgcatcgtg cgcatggtga cgctgaagct gctcgcgcgc
    12181 ggcctgcatc ccctggggct cctgcgcagc ctgcgccggc ggccgccgct cctggacgac
    12241 gaggacgccg aggagcgcgc gagcgcctac aagtcctagc gcgccgccgc ggcgcgcgtc
    12301 cgcgaacatc atgagaaccc actactgcgg caaggtcgac gccgcgctcc tcggcaagac
    12361 ggtcacgctc tacggctggg cgcaccgccg ccgcgaccat ggcggcgtca tcttcatcga
    12421 cttgcgcgac cgcgagggcc tgcttcaggt cgtgagcgat ccggaccggc gcgagacctt
    12481 cgccaccgcc gagaagatcc gcagcgagta cgtcctgaag gtgaccggcg tggtgcgccg
    12541 ccgcccggaa ggcaccgtga acccgaacct gcgctcgggc gaggtcgagg tgctcgcgca
    12601 cgagatcgag atcctcaacg cctcgctcac gccgccgttc cagatcgacg acgacaacct
    12661 ctcggagaac gtgcggctca cctaccgcgt gctcgacctg cgccgcgagg cgatgcagaa
    12721 gaacctgcgg ctgcgctatc gcttggcgat ggccgtccgc cggtacctgg acgagcaggg
    12781 cttcatcgac atcgagacgc cgatgctcac acgctcgacg cccgagggcg cgcgcgacta
    12841 cctcgtcccc tcgcgcgtgc acccggggga gttcttcgcg ctgccgcagt cgccgcagct
    12901 gttcaagcag atgctgatgg tcgcgggcta cgaccgctat taccagatcg tcaaatgctt
    12961 ccgcgacgag gacctgcgcg ccgaccgcca gcccgagttc acgcagatcg actgcgagac
    13021 ctcgttcctg ggcgaggccg agatccgggc gctgttcgag ggcatgatcc gcggcgtctt
    13081 ccgggaggcg atcggggtgt cgctgcccga gccgttcccg gtgatgacct acgcggaggc
    13141 gatgcgcgac tacgcttccg acaagcccga cctgcgcatc ccgctcaagc tgaccgagct
    13201 caccgacgcg atgaagtcgg tcgagttcaa ggtgttctcg ggaccggcga gcacgcccgg
    13261 ccaccgcgtc gcggggctgc tcgtcccggg cggcggcgcg ctcacgcgcg gcgagctcga
    13321 cgcctacggc gagtacgtcg cgcagttcgg cgccaagggc cttgcgtgga tcaagttcaa
    13381 cgaggtgaag aagggccgcg agggcatgca gtcgccgatc gtgaagaacc tctcggacgc
    13441 cgcgctcgcg acgatcgtcg agcgctcggg cgcgaaggac ggcgacctga tgttcttcgg
    13501 cgcgggccgc aacaaggtcg tgaacgacgc gctcggcgcg ctgcgcgtca agctcggcca
    13561 cgaccggggc ctggtgcagg cgggctggcg cgtgctctgg gtcgtcgact tcccgatgtt
    13621 cgagtacgac gaggaagaga agcgctggaa cgccatgcac catccgttca ccgcgcccaa
    13681 ggacggccac gaggagaagc tcgcgaccga tccgggcgcg gcgctcgcca aggcctacga
    13741 catggtgctg aacggctcgg agatcggcgg cgggtcggtg cggatccacc gcgaggaggt
    13801 gcagtcgaag gtgttccgcg cgatgtcgat ctcgcacgac gaggcccgga acaagttcgg
    13861 cttcctgctc gacgccctgc agtacggcgc gccgccgcac ggcggcatcg ccttcggcct
    13921 ggaccgcctc gccgcgctca tggccgggtg cgagtcgatc cgcgacgtga tcgcgttccc
    13981 gaagacgcag cgcgcgcagg acctcctggt gaaagcgccg tccgccgtcg acgagaagca
    14041 gctgcgcgag ctgcacatcc ggctgcgcaa gtcggaggag ccgaagtgaa ggcgatggcc
    14101 gacgcggcgc aggccggcgt cttccacctc gggcccgacg cacgcgccgc ggcgcaggcc
    14161 gcggcgtcgg cgggcctgct cgtcgcctac gtcgacatcg gccacgcgca cgacaaggag
    14221 catttcgccg agcacgtcgc cgaggcgctg cgcttcccgg gcggtcacga cacggactgg
    14281 gacaccttcg tgaaaggcgt caccgacctc tcgtggctcg aggccaaggg ctgggtcgtg
    14341 atcctcgaga agagcaagca tttctgcggc ggccaccgcc acgagttcga ggacgcgatg
    14401 cgcgtcatgt ccgatgccgc cgcgcactgg cgcgcgcgcg gcaggccgtt ctggaccctg
    14461 atcggcggtc cggaaggatg gaaatccggc tggcccgatc tgccttgagc tacaagatcc
    14521 cgcgctcggc gctcgtcgtc atccacacgc ccgacctgca ggtgctcatg atggagcgga
    14581 cctcctggcc ggggttctgg cagtcggtca cgggcggcct ggaccgcgag gacgagccgt
    14641 accgcgacgc ggcggtccgc gaggtgcgcg aggagaccgg catcgacgcg acgaaatacg
    14701 cgctcacgga ctggggcctc gagcgcgaat tcgagatctt cgccaggcac cgccaccgct
    14761 atgccccggg cgtggcgcgc aacctcgagc acgtcttcag cctcgaggtg cccgcgccgc
    14821 tcgaggtgcg gctcgagccg gccgagcacc tgcgctaccg gtggctgccg taccgcgagg
    14881 ccgccgcgct cacgatctcc tggagcaacc gcgacgcgat cctggcgctg ccggagcggc
    14941 gcgcgcaagt ctcccgcaag ccttgagccc ttgccgggcc ccggcgcgat gctgcatgct
    15001 gcgccatggc cgccccccgc ctcgccgcgc tgctcgcgct cgtcgtctgc gtcgcgccgt
    15061 gcgcgcccgc cgcggcccag gcgatcgcgc tcgagaccta ccgccagggc gacgcggtca
    15121 acgtctacgc cgaggtgcgg ctcgacgtcg acccgaagat cgcctgggat gtcctgtcgg
    15181 actacgatca cctcgccgaa ttcgtccccg acatgagcta ctctcgcgtc ctgtcgcgcg
    15241 acgggaacaa ggtgaccatc gagcagaagg gcgagttcgg cgtgctgttc ttccgccagc
    15301 cgatcgagct caagctcgag atcgtcgaga cgccgcaccg gacgatcgtc gcgcgcgccg
    15361 tgggcgggag cttccgcgag atgaccggcc gctacgacct cgaccgcgtc gaaggcggcg
    15421 gcgtgcgcat cggctacgcc gggcacttcg ttccggcgtt cccgctgccg acgttcttcg
    15481 ggatcatcgg cgtgcggcac accgcggcct cgcagctctc ggcgctggtc gacgagatcg
    15541 tgcgccgcga cgcgcgcgcg cgttccggcc actgagcggg cggccgcgca accgcttctt
    15601 tgatacgatt ggggaatgcc cgcggcgccg tccgcggccg tcgaaccgct tccgtcccaa
    15661 cgaatgaacc tgcacatcgc cacctacaac atccacaagg gcttcacgca tttcaaccgc
    15721 cgcatgatgg tccacgagct gcgcgaccag ctgaggtcgc tgggcgcgga catcgtgttc
    15781 ctgcaggaag tgcagggcat gcaccaccgc cacgcgtcgc gcttcgagaa ctggccgatc
    15841 gagccccagt acgagttcct cgccga
//
LOCUS       DATDMW010000064         6018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_764545, whole genome shotgun sequence.
ACCESSION   DATDMW010000064 DATDMW010000000
VERSION     DATDMW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764545"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VI319_02760"
     CDS             complement(<1..536)
                     /locus_tag="VI319_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6862796.1"
                     /translation="MNAPSDSRSDLRSTMLAIGQRARAASREVARASTDAKNRALLAA
                     AAAIRRDAGRLLAANAEDVAAARAGGKDSAFIDRLTLTPKSIEAMAAGLEEVAKLGDP
                     VGEVSELRERPSGIKVGRMRVPLGVIGIIYESRPNVTADAAALCLKSGNACILRGGSE
                     SVRSNLAIAACMHEGLRAG"
     gene            complement(655..1089)
                     /locus_tag="VI319_02765"
     CDS             complement(655..1089)
                     /locus_tag="VI319_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEY6862797.1"
                     /translation="MADAFNLRRFVLAQDPDYDSILGELRAGRKRGHWIWYVFPQILG
                     LGASAMSRRYAISGCDEARAYAEHAILGARLRECTQLVLDIEGRTAEQIFPHPDDLKF
                     RSSMTLFERCTADPALFRAALDKYFAGKPDPVTLERLDRPQR"
     gene            complement(1098..1457)
                     /locus_tag="VI319_02770"
     CDS             complement(1098..1457)
                     /locus_tag="VI319_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6862798.1"
                     /translation="MSDLDAATEMYKRGLGLQELGRFDDHDGFDGRMLGMPGAQFHFE
                     FTACRSHPVRPTPTAEDLLVFYVPDPGAWSDRCAAMLAAGFAAVPSFNPYWERSGRTF
                     QDRDGYRVVIERDDAPV"
     gene            complement(1487..2491)
                     /gene="holA"
                     /locus_tag="VI319_02775"
     CDS             complement(1487..2491)
                     /gene="holA"
                     /locus_tag="VI319_02775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY6862799.1"
                     /translation="MTQIRLDQLDAQLQRPPAALWAIHGDEPLLWLEAADAIRRAARK
                     AGYTQREVLAAERGFDWRALADAAASLSLFGDRRIVELRMSGKPGADGARAIEAYCAR
                     PPDDTVTIVSFPRVDRAAQSSPWFTALERAGVVVNVYPVERPRLPAWIAQRLKRQNQS
                     AGAQALQFLADSVEGNLLAAHQEIQKLALLAPEGELSFETVRDCVLNVARYDAGDLAA
                     AMLSGERARLARVLDGLRGEGEAEARLVWILAEEIRAVARVQEGLGSGRDLAALLSEN
                     RIWGDARKAAVGGAARKHKRKALEAALAHAARVDRIVKGVAQGDAWDELLQLGLRFAG
                     "
     gene            complement(2493..2987)
                     /gene="lptE"
                     /locus_tag="VI319_02780"
     CDS             complement(2493..2987)
                     /gene="lptE"
                     /locus_tag="VI319_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY6862800.1"
                     /translation="MTRILPIALALVLAGCGFHLRGQQQLPFKTLYVPGGSGLSLELK
                     RNLAAQARGTQLVDETKNADAVLTFTSQRTEKVILSFDTAGRVREYQLRYTVGFRLTD
                     AKGLVYIPTNEIRLTRDISFNDAQVLAKEAEEAQLYRDMQSDMVQQLLRRIAAAKTAK
                     PDLE"
     gene            complement(2987..5608)
                     /gene="leuS"
                     /locus_tag="VI319_02785"
     CDS             complement(2987..5608)
                     /gene="leuS"
                     /locus_tag="VI319_02785"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY6862801.1"
                     /translation="MQPLYHPDKIEREAQDHWERTGAFRAVEDASRAKFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVMTRYHRMRGENVLQPMGWDAFGLPAENAAMANAVPPAKWTY
                     DNIAYMKKQLKSLGFAIDWAREVTTCSPAYYRWNQWLFLRMLEKGLAYKTTGVVNWDP
                     VDQTVLANEQVIDGRGWRTGALVEKREIPMYYLRITAYAEELLASLERLPGWPERVKT
                     MQANWIGKSHGVNIGFPYELGGEKRLLRVFTTRADTLMGTTFCAVAAEHPLAAHAAKS
                     DPKLAAFVEECKRGTVMEAELAQLDKKGMPTGLSVTHPISGDRIPVWVGNYVLMTYGE
                     GAVMGVPAHDERDFAFAKKYGLPIRQVIDVDGKRYSTDAWQPWYADYGRCVHSGPYDG
                     LDYQAAVDAIAADLKAKRLGDKQVTWRLRDWGISRQRYWGTPIPIIHCPSCGDVPVPD
                     EQLPVVLPENLVPDGTGNPLAKTASFVNCACPKCGKPARRETDTMDTFVDSSWYFIRY
                     ACPDQAKAMVDEREKYWMAVDQYIGGIEHAILHLLYSRFWTKVMRDLGLATVDEPFTR
                     LLTQGMVLNEIFYRKGGEGRITYYNPADVDVKTDEQGRRTSAVLRADGKPVESDGIGT
                     MSKSKNNGVDPQMLIEEYGADTARFFIMATSPPEFTLLWSDAGVEGASRFLRRLWTFS
                     HRFSEELQGGVAQVPDAGSLTLSPALTEVRRQVHLQLRQATFDMERNQFNTVASACIK
                     ILNALDDAASGKADADARARHAVSKEGLSILLRVLSPIAPHICHTLWREFALGEDVLQ
                     APWPQPDAKALEQDEIELVLQVNGKLRGHLRAPKSAGREELEKMALAHDAVARHANGQ
                     PVRKVVVVPGRLVNVVV"
     gene            5735..>6018
                     /locus_tag="VI319_02790"
     CDS             5735..>6018
                     /locus_tag="VI319_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862802.1"
                     /translation="MTSIQAHVAALALALAGSAFAQGNPPAGAAKGPAPAAPAAAAPA
                     PAAPAAAPAPAAPAAAPAAAAPETAAPVAKPARKAKAAPPPGSVVQAVQAP"
BASE COUNT          876 a         2111 c         2103 g          928 t
ORIGIN      
        1 cccgcgcgca gcccctcgtg catgcacgcg gcgatcgcca ggttcgagcg caccgactcg
       61 gagccgccgc gcaggatgca cgcgttgccc gacttcaggc acagcgccgc cgcgtccgcg
      121 gtcacgttgg gccgcgactc gtagatgatg ccgatcaccc cgagcggcac gcgcatgcgc
      181 ccgaccttga tgcccgaggg ccgctcgcgc agctcgctca cctcgccgac cggatcgccg
      241 agcttcgcca cctcctccag gcccgcggcc atggcctcga tggacttcgg ggtgagcgtc
      301 aggcggtcga tgaaggcgga gtccttgcct ccggcgcgcg cggcggcgac atcctcggcg
      361 ttcgcggcga gcagcctgcc ggcgtcgcgc cggatcgccg cggcggcggc gagcagcgcg
      421 cggttcttcg cgtccgtcga tgcgcgcgcc acctcgcgcg aggctgcgcg cgcgcgctgg
      481 ccgatcgcga gcatggtgct gcgcaggtcg gagcgggagt cggagggcgc gttcataagt
      541 gaattctacg tcggccgggc cgggaccgtc gttccggacc gaagcgaccc gccggaggct
      601 ggatcccgtc gggcgcggtg tcgtctcgaa gctgtgggtc gcggcggcga cgcatcatcg
      661 ctgcggccgg tcgaggcgct cgagcgtcac ggggtcgggc ttccccgcga agtacttgtc
      721 gagcgcggcg cggaacagcg ccgggtccgc ggtgcagcgc tcgaagaggg tcatcgagga
      781 tcggaacttc aggtcgtcgg gatgcgggaa gatctgctcg gcggtccgac cctcgatgtc
      841 gagcacgagc tgcgtgcact cgcgcagccg cgcgccgagg atcgcgtgct cggcgtacgc
      901 gcgcgcctcg tcgcacccgg agatcgcgta cctgcgcgac atcgcgctcg cgccgagccc
      961 gagaatctgg gggaagacgt accagatcca gtggccgcgc ttgcggcccg cgcgcagctc
     1021 gcccaggatc gaatcgtagt ccgggtcctg cgcgaggacg aagcggcgga ggttgaaggc
     1081 gtcggccatg gctcgattca aaccggggcg tcgtcgcgct cgatgacgac acggtagccg
     1141 tcgcgatcct ggaacgtgcg gcccgagcgc tcccaataag ggttgaacga cgggacggcc
     1201 gcgaagcccg ccgccagcat cgccgcgcag cggtcgctcc aggccccggg gtccggcaca
     1261 tagaacacga gcaggtcctc cgctgtcggg gtcggccgga ccgggtgcga gcggcacgcg
     1321 gtgaactcga aatgaaactg cgcgccgggc atgccgagca tgcgcccgtc gaaaccgtcg
     1381 tgatcgtcga atcgcccgag ctcctgcagc cccaggccgc gcttgtacat ctcggtggcg
     1441 gcatctaggt cgctcaccgg tcgtgcaacg cggagaagca tcgaagtcag cccgcgaagc
     1501 gcagcccgag ctgcagcagc tcgtcccacg catcgccctg cgccacgccc ttcacgatgc
     1561 ggtcgacgcg cgcggcgtgc gcgagcgcgg cctcgagcgc cttgcgcttg tgcttgcgcg
     1621 cggcgccgcc gacggcggcc ttgcgcgcgt cgccccagat gcggttctcg ctcagcagcg
     1681 ccgcgagatc gcgacccgag ccgagtcctt cctgcacgcg cgcgacggcg cggatctcct
     1741 cggcgaggat ccacacgagg cgcgcctcgg cctcgccttc gccgcgcagg ccgtcgagca
     1801 cgcgcgcgag gcgcgctcgc tcgcccgaga gcatggccgc ggccaggtcg cccgcgtcgt
     1861 agcgcgccac gttgaggacg cagtcgcgca cggtctcgaa cgagagctcg ccctcgggag
     1921 cgaggagcgc gagcttctgg atctcctggt gcgcggcgag gaggttgcct tcgacgctgt
     1981 cggcgaggaa ctgcagcgcc tgcgcgcccg cgctctggtt ctgccgcttg aggcgctgcg
     2041 cgatccacgc gggcagccgg gggcgctcga ccgggtacac gttgacgacg acgccggcgc
     2101 gctcgagcgc cgtgaaccag ggcgacgact gcgcggcgcg gtcgacgcgc gggaacgaga
     2161 cgatcgtcac cgtgtcgtcg ggcggccgcg cgcagtaggc ctcgatcgcc ctggcgccgt
     2221 ccgcgcccgg cttgccggac atgcgcagct ccacgatgcg ccggtccccg aagagcgaga
     2281 gcgacgcggc cgcgtccgcg agcgcgcgcc agtcgaaccc tcgctcggcg gcgagcacct
     2341 cgcgctgcgt gtaccctgcc ttgcgcgcgg cgcggcgtat ggcgtcggcc gcctccagcc
     2401 agagcagcgg ctcgtcgccg tggatcgccc acagcgcggc gggcgggcgc tgcagctgcg
     2461 cgtcgagctg atccaggcgg atctgcgtca cgtcattcga ggtcgggctt ggcggtcttc
     2521 gcggccgcga tgcggcgcag gagctgctgc accatgtccg actgcatgtc gcggtagagc
     2581 tgcgcctcct ccgcctcctt ggcgaggacc tgcgcgtcgt tgaacgagat gtcgcgcgtc
     2641 agccggatct cgttcgtcgg gatgtacacg agacccttgg catcggtcag ccggaagccg
     2701 accgtgtagc gcagctggta ctcgcgcacg cggccggccg tgtcgaacga gaggatcacc
     2761 ttctcggtcc tttggctcgt gaacgtcagc acggcgtccg cattcttggt ctcgtcgacc
     2821 agctgcgtgc ccctagcctg tgcggcgaga ttgcgcttca gctcgagcga aaggcccgat
     2881 ccgcccggca cgtagagcgt cttgaaaggc agctgctgct ggccgcgcag atggaagccg
     2941 cacccggcga gaacgagcgc gagcgcgatc ggcaggatgc gcgtcatcac accaccacgt
     3001 tgacgagacg gccgggcacc acgaccacct tgcgcaccgg ctggccgttc gcgtggcggg
     3061 cgactgcgtc gtgcgcgagc gccatcttct cgagctcctc gcgcccggcg gacttgggcg
     3121 cgcgcaggtg gccgcgcagc ttgccgttca cctgcaggac cagctcgatc tcgtcctgct
     3181 ccagggcctt ggcgtcgggc tgcggccagg gggcctggag cacatcttcg cccagcgcaa
     3241 attcgcgcca cagggtgtga cagatgtgcg gcgcgatcgg agacaggacc cgaagcagta
     3301 tggagagccc ctccttggac acggcgtgtc gggcgcgcgc atcggcgtcc gccttccccg
     3361 atgccgcgtc gtccaatgcg ttcaggatct tgatgcaggc ggacgcgacc gtgttgaact
     3421 ggttgcgctc catgtcgaag gtggcttgcc gaagctgcag gtggacttgg cgccgcacct
     3481 cggtcaacgc gggcgaaagg gtcagggagc cggcgtccgg aacctgcgcc acgccgccct
     3541 gcaactcttc cgagaaacga tgcgagaacg tccagagccg gcgaaggaac cgcgacgcgc
     3601 cttcgacacc ggcatcggac cacaggagcg tgaactccgg cgggctcgtc gccatgatga
     3661 agaatcgcgc cgtgtccgcc ccgtactcct cgatcagcat ctgcggatcg accccgttgt
     3721 tcttcgactt cgacatcgtg ccgatgccgt cggactcgac cggcttgccg tcggcgcgca
     3781 gcaccgcgct cgtgcgccgg ccctgctcgt cggtcttcac gtcgacgtcg gccgggttgt
     3841 agtaagtgat ccggccttcg ccgcccttgc gatagaagat ctcgttcagc accatgccct
     3901 gcgtgagcag gcgcgtgaac ggctcgtcga cggtcgcgag ccccaggtcg cgcatgacct
     3961 tggtccagaa gcgcgagtag agcaggtgca ggatcgcgtg ctcgatgccg ccgatgtact
     4021 ggtcgaccgc catccagtac ttctcgcgct catcgaccat cgccttcgcc tggtcggggc
     4081 aggcgtagcg gatgaagtac caggacgagt cgacgaaggt gtccatcgta tcggtctcgc
     4141 gccgggcggg cttgccgcac ttcgggcacg cgcagttaac gaacgacgcg gtcttcgcga
     4201 gcgggttgcc ggtgccgtcg ggcacgaggt tctcgggcag taccaccggc agctgttcgt
     4261 cgggcaccgg cacgtcgccg cacgagggac aatggatgat cgggatcggc gtgccccagt
     4321 agcgctggcg cgagatgccc cagtcgcgca ggcgccaggt gacctgcttg tcgccgaggc
     4381 gcttcgcctt caggtcggcc gcgatggcgt cgaccgccgc ctgatagtcg aggccgtcgt
     4441 acgggcccga gtggacgcaa cgcccgtagt cggcgtacca cggctgccac gcgtcggtcg
     4501 agtagcgctt gccgtcgacg tcgatcacct ggcggatcgg caggccgtac ttcttcgcga
     4561 acgcgaagtc gcgctcgtcg tgcgcgggca cgcccatcac cgcgccttcg ccgtaggtca
     4621 taagcacgta attgccgacc cacaccggga tcctgtctcc ggagatcggg tgcgtcacgg
     4681 acaatcccgt cggcatgccc ttcttgtcga gctgcgcgag ctcggcctcc atcacggtgc
     4741 cgcgcttgca ctcctcgacg aatgccgcga gcttcggatc cgatttcgcc gcgtgcgcgg
     4801 caagcgggtg ctcggccgcg accgcgcaga aggtcgtgcc catcagcgtg tcggcgcgcg
     4861 tggtgaatac gcgcagcagc ctcttctcgc cgccgagctc gtagggaaag ccgatgttca
     4921 cgccgtggct cttgccgatc cagttcgcct gcatcgtctt cacgcgctcg ggccagccgg
     4981 gcagccgctc gagcgaggcg agcagctcct ccgcgtacgc ggtgatccgc aggtagtaca
     5041 tcgggatctc gcgcttctcc accagcgcgc cggtgcgcca gccgcggccg tcgatcacct
     5101 gctcgttcgc gagcacggtc tggtcgacgg gatcccagtt gacgacgccg gtcgtcttgt
     5161 acgcgaggcc cttctcgagc atgcgcagga acagccactg gttccagcgg tagtacgcgg
     5221 gcgagcaggt cgtgacttcg cgcgcccagt cgatcgcgaa gccgagcgac ttgagctgtt
     5281 tcttcatgta cgcgatgttg tcgtacgtcc acttcgccgg cggcaccgcg ttcgccatcg
     5341 ccgcgttctc ggccggcagg ccgaacgcgt cccagcccat cggctgcagc acgttctcgc
     5401 cgcgcatgcg gtgatagcgg gtcatcacgt cgccgatggt atagttgcgc acgtgaccca
     5461 tgtgcagctt gccggacgga tacgggaaca tcgacaggca gtagaacttc gctcggctcg
     5521 cgtcctcgac cgcgcggaaa gcgccggtgc gctcccaatg gtcctgcgcc tcgcgctcga
     5581 tcttgtcggg gtgatacagg ggctgcatcg gggatcggcc ggatgtatac ggcaaaaccg
     5641 aaaacgtgaa ttataaccgc cgcgtcgcac gggccccgtt catcgggcgt tgcccgtcgc
     5701 gatatgaacg ccgacgaaaa aacgggagga ctccatgacc agcatccagg cccatgtcgc
     5761 cgcgctcgct cttgcgctcg ccggctcggc gttcgcgcaa ggcaatccgc ctgcgggcgc
     5821 ggccaagggg cccgcaccgg cagcgcccgc ggcggcagcg cccgcacccg cagcaccagc
     5881 ggcagcgccc gcacccgcag caccagcggc agcgcccgcg gccgctgcgc ccgagacggc
     5941 ggcgcccgtg gcgaagcccg cgcgcaaggc gaaggcggcg ccgcctcccg gctcggtggt
     6001 gcaggcggtc caggcgcc
//
LOCUS       DATDMW010000065         5416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1137487, whole genome shotgun sequence.
ACCESSION   DATDMW010000065 DATDMW010000000
VERSION     DATDMW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137487"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /gene="tatC"
                     /locus_tag="VI319_02795"
     CDS             complement(<1..548)
                     /gene="tatC"
                     /locus_tag="VI319_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY6862803.1"
                     /translation="MSSQDTFISHLVELRDRMLRAILAVLLVFLCLFPWAKDLYALLA
                     MPLLAVLPKGGQMIATDVVGVFLVPVKVAFLVAFVVALPYVLYQAWAFVAPGLYAHER
                     RLVLPLVVASSALFIVGMSFAYFLVFPVVFKFMASIAPEGVAWMTDIDKYLSFVLTSF
                     IAFGVTFEVPVVVIVLVRVGIVS"
     gene            complement(558..1019)
                     /gene="tatB"
                     /locus_tag="VI319_02800"
     CDS             complement(558..1019)
                     /gene="tatB"
                     /locus_tag="VI319_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY6862804.1"
                     /translation="MFDVGFSELMVIAVVALIVIGPERLPKVARTLGHLVGRLQRYVN
                     DVKADINREIELDELRKFKTTFEDAAREMEHTVHNEAAKAESEMNAIAQSVDAAATGG
                     LTHPGTASGELQAPKTEVDPAARPVEEAAKAPKLPEAPGAEQPQTAQKSPA"
     gene            complement(1075..1293)
                     /gene="tatA"
                     /locus_tag="VI319_02805"
     CDS             complement(1075..1293)
                     /gene="tatA"
                     /locus_tag="VI319_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEY6862805.1"
                     /translation="MGTWSIWHWLIVLVIVLLIFGTKKLKNIGADLGGAVRGFKEGMK
                     SGDDDKPEAPSQITGRTIDAEVKDKAKS"
     gene            complement(1380..1727)
                     /locus_tag="VI319_02810"
     CDS             complement(1380..1727)
                     /locus_tag="VI319_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY6862806.1"
                     /translation="MDCIFCKIVRGEIPSRKVYEDDEVLAFHDIHPLAPVHFMIIPKH
                     HLASLADADMSHQALLGKMLALAPRLAREQGSPDGFRTIVNTGRVGAQEVYHLHMHVI
                     GGPAPLGPMIRRG"
     gene            complement(1752..2102)
                     /locus_tag="VI319_02815"
     CDS             complement(1752..2102)
                     /locus_tag="VI319_02815"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY6862807.1"
                     /translation="MSDILERLADVVESRKAGDPDASYVARLFARGEDAILKKIGEEA
                     TETVMAAKDGDRLRIVGETADLWFHCLIMLAQHGLRPSDVLAELRRREGISGIDEKAA
                     RADDARIEAERKRK"
     gene            complement(2113..2502)
                     /gene="hisI"
                     /locus_tag="VI319_02820"
     CDS             complement(2113..2502)
                     /gene="hisI"
                     /locus_tag="VI319_02820"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY6862808.1"
                     /translation="MDDDWLDTVKWDANGLVPAIAQDAATRKVLMLAWMNRESLAKTV
                     ATGEAHYWSRSRRRLWRKGEESGHVQKVRAVRIDCDEDAVLLEVEQAGGIACHTGRER
                     CFFRKLEGRRWVETEAVVRDPREIYDK"
     gene            complement(2513..3283)
                     /gene="hisF"
                     /locus_tag="VI319_02825"
     CDS             complement(2513..3283)
                     /gene="hisF"
                     /locus_tag="VI319_02825"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY6862809.1"
                     /translation="MGLARRIIPCLDVDRGRVVKGVNFVGLRDAGDPVEIARRYDEQG
                     ADELTFLDITASSDERDLIVQAVERVARQVFIPLTVGGGVRRVEDVRRLLNAGADKVS
                     INTAAVQNPALVAEAAGRYGSQCIVVAVDAKRVADGSDSRWEVFTHGGRRATGLDAVD
                     WARRMQQAGAGEILLTSMDRDGTREGFDIPLTRAVADAVDVPVIASGGVGSLEHLAQG
                     VLEGRADAVLAASIFHFGEFSVRQAKEHLARRGIEVRL"
     gene            complement(3323..4072)
                     /gene="hisA"
                     /locus_tag="VI319_02830"
     CDS             complement(3323..4072)
                     /gene="hisA"
                     /locus_tag="VI319_02830"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY6862810.1"
                     /translation="MLIIPAIDIKDGQCVRLKQGRMEDATVFSEDPGGMAGHWKSEGA
                     RRLHVVDLNGAAAGKPKNVPAIRAILQAAGDAVPVQLGGGIRDLDTIEQYLDLGVTYA
                     IIGTAAVKNPGFLQDACSAFAGHVIVALDAKDGKVAVEGWSKMTGHDVVDLAKKFQDY
                     GVEAVIYTDIGRDGMLTGVNVDATLKLARQLRVPVIASGGLTSLRDIESLCAIESEGI
                     TGVITGRAIYQGTLDFKKAQAAIDKLAKEKE"
     gene            complement(4134..4778)
                     /gene="hisH"
                     /locus_tag="VI319_02835"
     CDS             complement(4134..4778)
                     /gene="hisH"
                     /locus_tag="VI319_02835"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY6862811.1"
                     /translation="MADRIAVIDYGMGNLYSVSRALEHVAESAQVELTSDPARVASAD
                     RVVFPGQGAMPDCMREMDARGLRPAVLDAARNKPFLGICIGLQMLFDSSEEGETEGLG
                     VLRGHVRRFAPAEMRAADGSRLKVPHMGWNQVAQQPGRHPLWSGIADDERFYFVHSYF
                     PEALDPEVVQGVATYGVPFTCAVARDNIFAVQFHPEKSQSAGLKLLANFIHWTP"
     gene            complement(4799..5410)
                     /gene="hisB"
                     /locus_tag="VI319_02840"
     CDS             complement(4799..5410)
                     /gene="hisB"
                     /locus_tag="VI319_02840"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY6862812.1"
                     /translation="MVHPPSPLGRSAAVQRDTRETQIRVELDLDGRGEARLSTGLPFL
                     DHMLDQVARHGMLDLDIKAKGDLHIDGHHTVEDIGITLGQAVARAVGDKRGIRRYGHA
                     YVPLDEALSRVVIDFSGRPGLEYRAEFKRALIGEFDVDLVHEFFQGFVNHAQVTLHVD
                     NLRGDNAHHQCETMFKAFARALRMALELDPRAGDAVPSTKGTL"
BASE COUNT          829 a         1872 c         1833 g          882 t
ORIGIN      
        1 gagacgatgc cgacgcgcac cagaacgatg acgacgacag gcacctcgaa agtcacgccg
       61 aaggcgatga acgaggtcag cacgaacgac aggtacttgt cgatgtcggt catccaggcg
      121 acgccctcgg gcgcgatcga cgccatgaac ttgaacacga ccggaaacac caggaagtac
      181 gcgaaggaca tgccgacgat gaacagcgcc gagctcgcga ccacgagcgg cagcaccagg
      241 cgccgctcgt gcgcgtacag gcccggggcg acgaacgccc acgcctggta gagcacgtac
      301 gggagcgcca cgacgaacgc gaccaggaac gccaccttca ccggcacgag gaatacgccg
      361 accacgtcgg tggcgatcat ctggccgccc ttcggcagca ccgcgagcag gggcatcgcg
      421 agcagcgcgt acaggtcctt cgcccagggg aacaggcaca ggaacacgag cagcaccgcg
      481 agaatcgccc gcagcatgcg gtcgcgcagc tcgacgaggt gcgagatgaa ggtgtcctgg
      541 ctgctcatgc tgccaggcta ggctggagac ttctgcgcgg tctgcggctg ctcggcgccc
      601 ggcgcctccg ggagcttcgg cgccttcgcc gcctcctcga ccgggcgcgc cgccgggtcg
      661 acctcggtct taggagcctg cagctcgccc gacgccgtgc ccggatgagt gaggccgccg
      721 gtcgccgcgg cgtccaccga ttgcgcgatg gcgttcatct cggactcggc cttcgcggct
      781 tcgttgtgga cggtgtgctc catctcgcgg gccgcgtcct cgaacgtcgt cttgaacttg
      841 cgcagctcgt cgagctcgat ctcgcggttg atgtcggcct tgacgtcgtt gacgtagcgc
      901 tgcaggcggc caacgagatg acccagggtg cgggccacct tgggcagccg ctcggggccg
      961 atgacgatga gcgccaccac ggcgatgacc atcagctcgg agaatccgac gtcgaacatg
     1021 gggatcagcg gaagcgctgc cgccggcgcg cggcggccgg gccgagcgtc acgctcagct
     1081 cttcgccttg tccttcactt cggcgtcgat cgtgcggccg gtgatctggc tcggggcctc
     1141 cggcttgtcg tcgtcgccgc tcttcatgcc ttccttgaat ccgcgcaccg cgccgcccag
     1201 gtcggccccg atgttcttca gcttcttcgt tccgaagatc agcaggacga tcaccaggac
     1261 gatgagccag tgccagatgc tccaggttcc catgactgca tgctccttgt gggcgcccgg
     1321 gacgccgggc cgcctcgatt tcagtggggg agagccgggc ccgtggccgg gccgcggcct
     1381 caaccgcgtc ggatcatcgg gcccagcggg gccgggccgc cgatgacatg catgtgtaaa
     1441 tgatacacct cttgcgcccc cacccggcca gtattgacga tggtgcggaa gccgtcgggg
     1501 gagccttgct cgcgcgcgag ccgcggcgcc agggccagca tcttcccgag gagggcctga
     1561 tggctcatgt cggcgtcggc gagcgacgcg aggtgatgct tcgggatgat catgaaatgc
     1621 accggcgcga gcgggtggat gtcgtggaac gcgaggacct cgtcgtcctc gtagaccttg
     1681 cggctgggga tctcgccgcg cacgatcttg cagaagatgc agtccatgct cgcggtctca
     1741 gatgcggccg ctcatttgcg cttgcgctcg gcctcgatgc gggcgtcgtc cgcgcgcgcg
     1801 gccttctcgt cgattcccga gatgccttcg cggcgtcgca gctcggcgag cacgtccgag
     1861 ggccgcagcc cgtgctgcgc gagcatgatc aggcagtgga accacagatc cgcggtctcg
     1921 ccgacgatgc ggagccggtc gccgtccttg gcggccatca ccgtctcggt cgcttcctcg
     1981 ccgattttct tgaggatcgc gtcctcgccg cgcgcgaaca gccgcgcgac ataagaggcg
     2041 tcggggtcgc ccgccttgcg gctctcgacg acgtcggcga gcctctcgag gatgtcgctc
     2101 atatctcgaa gctcatttgt cgtagatctc ccggggatcg cgcacgaccg cctcggtctc
     2161 gacccagcgc cggccttcga gcttgcggaa gaagcagcgc tcgcggcccg tgtggcaggc
     2221 gatgccgccc gcctgctcca cctcgagcag cactgcgtcc tcgtcgcagt cgatgcgcac
     2281 cgcgcgcacc ttctgcacgt gcccggactc ctcgcccttg cgccagagcc tgcggcgcga
     2341 gcgcgaccag tagtgcgcct cgcccgtggc gacggtcttc gcgagcgact cgcggttcat
     2401 ccacgcgagc atcagcacct tgcgcgtcgc ggcgtcctgc gcgatcgcgg gcaccaagcc
     2461 gttcgcatcc cacttcacgg tgtcgagcca gtcgtcgtcc atgacgcagg cactatagcc
     2521 taacctcgat gccgcggcgc gcgaggtgct ctttcgcctg gcgcacgctg aattcgccga
     2581 agtggaagat cgacgccgcg agaacggcgt cggcgcgtcc ctcgagcacg ccctgcgcga
     2641 ggtgctcgag gctccccacc ccgccgctcg cgatcaccgg gacgtcgacc gcgtcggcca
     2701 ccgcgcgcgt gagcgggatg tcgaagccct cgcgcgtgcc gtcccggtcc atgctggtca
     2761 gcaggatctc tccggcgccg gcctgctgca tcctgcgcgc ccagtccacc gcgtccaggc
     2821 cggtcgcgcg ccggccgccg tgcgtgaaca cctcccatcg ggaatcgctc ccgtccgcga
     2881 cgcgcttggc gtcgaccgcg accacgatgc attgcgatcc gtagcgcccc gccgcctcgg
     2941 cgacgagcgc cgggttctgc accgcggcgg tgttgatcga caccttgtcg gcgccggcat
     3001 tgagcaggcg ccgcacgtcc tcgacgcggc gcacgccgcc gccgacggtg agcgggatga
     3061 acacctgccg cgccacgcgc tcgaccgcct gcacgatgag atcgcgctcg tccgagctcg
     3121 ccgtgatgtc gaggaaggtg agctcgtccg cgccctgttc gtcgtagcga cgcgcgatct
     3181 cgaccgggtc gccggcgtcg cgcagaccga cgaagttgac gcccttcacc acgcggccgc
     3241 ggtcgacgtc caggcaggga atgatgcgcc gggcgagacc catggcgtcg ggatcgaagc
     3301 accctcggac gggtgttcga ccctactcct tctccttcgc gagcttgtcg atcgccgcct
     3361 gggccttctt gaagtcgagc gtcccctggt agatcgcgcg gccggtgatg acgccggtga
     3421 tgccctcgga ctcgatcgcg cacagcgact cgatatccct gaggctggtg agtccgccgc
     3481 tcgcgatcac cggcacgcgc agctggcgcg cgagcttcag ggtcgcatcg acattcacgc
     3541 cggtgagcat gccgtcgcgc ccgatgtcgg tgtagatcac cgcctcgacg ccgtagtcct
     3601 ggaatttctt cgccagatcg accacgtcgt ggccggtcat cttcgaccat ccctcgacgg
     3661 cgactttgcc gtctttcgcg tcgagcgcga cgatgacgtg cccggcgaac gcgctgcacg
     3721 cgtcctggag gaaccccgga ttcttcaccg cggcggtgcc gatgatcgcg taggtcacgc
     3781 cgagatcgag gtactgctcg atcgtgtcga gatcacggat gccgccgccg agctgcaccg
     3841 ggaccgcgtc tcccgccgcc tgcaggatcg cccggatcgc cggcacgttc ttcggcttgc
     3901 cggcggccgc gccgttcagg tcgacgacgt gcagccggcg cgcgccttcc gatttccagt
     3961 gacccgccat cccgccgggg tcctcggaga acaccgtcgc gtcctccatc ctgccctgct
     4021 tgaggcggac gcactgcccg tccttgatat cgatcgccgg gatgatcagc attggggtaa
     4081 cgcgggacgc tggagagaat cggagcgcgg aaggaaccgc ggaaaagggc gattcagggt
     4141 gtccagtgga tgaagttggc gagcagcttc aggccggcgg attggctttt ttcggggtgg
     4201 aactgtaccg cgaaaatatt atcccttgcc accgcgcagg taaagggtac cccgtaggtc
     4261 gcgacgccct gcaccacctc ggggtcgagc gcctcgggaa agtagctgtg gacgaagtag
     4321 aagcgctcgt cgtcggcgat gccgctccag agcggatgcc gaccgggctg ctgagccacc
     4381 tggttccagc ccatgtgcgg gactttgagc cgggagccgt cggccgcgcg catttcggcc
     4441 ggggcgaagc gccgcacgtg cccccgaagc acgcccagcc cctcggtttc cccctcctcg
     4501 ctcgagtcga acagcatctg cagtccgatg cagatgccca gaaacggctt gttgcgcgcc
     4561 gcatcgagca ccgcgggccg caggccgcgc gcgtccatct cgcgcatgca gtcgggcatg
     4621 gcgccctggc ccgggaagac aacccggtcg gccgaggcca cgcgcgcggg atccgaagtg
     4681 agctcgacct gggcggattc ggcgacgtgc tcgagcgccc gggacaccga gtagaggttg
     4741 cccatgccgt aatcgatcac cgcgatgcga tcagccatcg gagacgtgca aaggaggact
     4801 acagcgtccc cttggtcgaa gggacggcgt cgccggcgcg cgggtcgagc tcgagcgcca
     4861 tgcgcagcgc ccgcgcgaac gccttgaaca tcgtctcgca ctggtggtgc gcgttgtcgc
     4921 cgcgcaggtt gtcgacgtgg agcgtgacct gcgcgtggtt gacgaatccc tggaagaact
     4981 cgtgcacgag gtcgacgtcg aactcgccga tcagcgcgcg cttgaactcg gcgcggtact
     5041 cgaggcccgg gcgcccggag aagtcgatca cgacccgtga cagggcttcg tcgagcggca
     5101 cgtaggcgtg gccgtagcgc cggatgccgc gcttgtcgcc gaccgcgcgc gcgaccgcct
     5161 gcccgagcgt gattccgatg tcctcgacgg tgtggtgccc gtcgatgtgc agatcgccct
     5221 tcgccttgat gtcgaggtcg agcatgccgt ggcgggccac ctggtcgagc atgtggtcga
     5281 ggaagggcag gccggtggac aggcgcgcct caccccgacc gtccagatcg agttcgacgc
     5341 ggatctgcgt ttcccgggtg tcgcgctgaa ccgctgcgga gcgtcccaac gggctagggg
     5401 ggtgtaccaa gggaag
//
LOCUS       DATDMW010000066         5646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_639535, whole genome shotgun sequence.
ACCESSION   DATDMW010000066 DATDMW010000000
VERSION     DATDMW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639535"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VI319_02845"
     CDS             <1..775
                     /locus_tag="VI319_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862813.1"
                     /translation="FMTQAAKQGRGTFTYIGRIGEVRDKMTELFAKLESPVLSGIRID
                     WPDGAGVESWPARIPDLYAGEPVVVTASLDRLEGVARVSGARGAAPWRAEIALDPRIG
                     SEAAGVGILWARDKIAALSEAMPAHAGPDARGEIVKLALAHHLVTKYTSLVAVDRAPA
                     RPAGEDLKTMAIPTNLPEGQSYEAIFGPAIGELPRGATDSRLHIAVGLAFMLCALLLN
                     IARRMTGGPTDPRRRVCNRESPSPLSSADSSASASFYAA"
     gene            902..1663
                     /locus_tag="VI319_02850"
     CDS             902..1663
                     /locus_tag="VI319_02850"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HEY6862814.1"
                     /translation="MNRTIRSDRDDFLGVESAHPATLPGLVWPPKRTSTPPRGRSRAG
                     RGGGRAAKWLALLAALLIAAGAFELGRGYYIQAKAQVAQLLIRSAWTRTLAGERRVKP
                     WPWADTWPVARLQVTDRSIDLFVLAGADGRAIAFGPGHVFGTAMPGERGNSVIGGHRD
                     THLAFLRDLAPGSLLSVQRPDGMRRWYRVKTAQVVDKGDTWVTRQDGPTRLTLVTCYP
                     FDALRAGGDQRYVVFANAVERAAEAPAPLPKGQPG"
     gene            1759..2643
                     /locus_tag="VI319_02855"
     CDS             1759..2643
                     /locus_tag="VI319_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862815.1"
                     /translation="MVTTRTLKPEGRSAHFNWAGLGRVAPQGLVNARLLAHHALQWPA
                     RCARANIKPAADDSHSSFDWDPGLAALVTQPLKTPAGDVRVGLRLSGLAILLVRNGAI
                     LDVLQLNGKPEQVAGVWIDAKLQALGLRAASDVRLPYGMPDHAVAQGARYDLYLQERE
                     LGELARWFAGASAALEDARERLADLRPSPVRLWPHHFDIASVAALDAPGADGARSIGF
                     GLSPGDEHYAQPYVYVSPSPSFERLPDAPKPGRWHTDGWFGAVATGDALLEQVNRGQA
                     VLRFILEAVDVGRKRFGA"
     gene            complement(2644..3603)
                     /locus_tag="VI319_02860"
     CDS             complement(2644..3603)
                     /locus_tag="VI319_02860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862816.1"
                     /translation="MGSSARWGMALAALLAISAPAFSQSYPARPIRVILPFAGGTDAV
                     ARLLSAKMSPAIGQQLVPEPRLGAGGNIAHELVAKAPPDGYTLLMAAPPLVINPHLNK
                     VNYDPLRDLAPIALLTAIPNVLVVHPSVPAKTMDELIALARRSPGKLTYASGGVGSSN
                     HLAGELLKSMTKIDILHVPYKSATIALTGALAHEVDVVIVASSSVAAMVKDGRMRALA
                     VLDNKRVDALPGVPTMAEVGLPQLVVVNWYILLAPAGTPRAIVDFLNREAVKAMNDPE
                     TRTRLANLGGEPASGTPEQAAEMLRREYEQWGRVIREAGIKGE"
     gene            3767..5317
                     /gene="gshA"
                     /locus_tag="VI319_02865"
     CDS             3767..5317
                     /gene="gshA"
                     /locus_tag="VI319_02865"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY6862817.1"
                     /translation="MSGAGAFRARLAALTPAMLRGLNRGIEKESLRVEPDGVLALTPH
                     PRALGSALTHPHITTDFSESLIELITGVHTEVESCLDELERIHQFVYRHIGDEMLWAA
                     SMPCCLPNEQQIPIARYGTSNVAQAKTVYRVGLTHRYGKRMQMIAGLHYNWSMPESAW
                     KLPGFPDASTGYFALIRNFRRNAWLLMLLFGASPAVCSTFVEGRPHGLQPFGPGTLYL
                     PHATSLRMGRLGYLSEAQDKLLVSYNSLGSYTASLYDAMTKPYPAYEKIGIRDGNGYR
                     QLATSLLQIENEFYSTIRPKRVINPGERPLHALRERGVEYVEVRAMDLDPFSPVGIRA
                     QTVRFLDVFLLHCLLADSPLDTAEEIESVGRNKQRIAARGREPGLTLDRGGQVVTVRD
                     WGADIVRECEPIADALDAAHGGSAHRDALAAATESLENPDSLPSARMLAEIEASYDKE
                     YPPFAYAKSIEHRRRLMEKPLPKEAEAAFERMAKESMEEQKAIEKADKISFEEYRERY
                     MEHEKLRA"
BASE COUNT          873 a         2029 c         1913 g          831 t
ORIGIN      
        1 cttcatgacg caggcggcaa aacaagggcg cggcacgttc acctatatcg gcaggatcgg
       61 ggaggttcgc gacaagatga ccgagctatt tgccaagctc gagtcgcccg tgctctcggg
      121 catccgcatc gactggccgg acggcgccgg cgtcgagtcc tggcccgcgc gcattcccga
      181 tctctatgcg ggagaacccg tggtggtgac ggcctcgctc gaccggctgg aaggcgtcgc
      241 gcgcgtctcc ggcgctcgcg gcgcggcacc ctggcgggcc gagatcgcgc tcgatccccg
      301 gatcgggagc gaagcggcgg gcgtcggcat cctatgggcg cgggacaaga tcgccgctct
      361 ttcggaggcg atgcccgccc atgccggccc ggacgcacgc ggtgagattg taaagttagc
      421 gcttgcccac cacttggtca cgaaatacac gagcctggtc gccgtcgacc gcgccccggc
      481 gcgaccggcg ggcgaggacc tgaagacgat ggcaatcccg acgaacctgc ccgaaggcca
      541 gagctacgag gcgatcttcg ggccggcgat cggcgagctg ccgcgcgggg cgaccgacag
      601 ccggctgcat atcgcggtcg ggctcgcgtt catgctgtgc gcgctgctcc tgaacatcgc
      661 ccgccggatg accggcgggc cgaccgaccc gcggcggcga gtctgcaacc gcgagagccc
      721 ctccccgctc tcgtctgcgg actcgtccgc ttcggcttct ttttatgctg cgtagtccat
      781 atggcgggct gatgtgtccg gtttccggcc gttttccctg catctttgga actactgttg
      841 cggaatagcc ctccaattgg tgcactaggg ggcgcaggta cacgttaggc agggaatgca
      901 gatgaacaga accatccggt ccgaccgcga cgacttcctg ggcgtcgaat ccgcccatcc
      961 cgcgacgctt cccgggctgg tctggcctcc caagcgaaca tcgaccccgc cccgcggccg
     1021 gtcccgcgcg gggcggggcg gagggcgagc cgcgaagtgg ctcgccctcc tcgccgccct
     1081 cctgatcgcc gccggcgcct tcgagctcgg gcgcggctac tacatccagg ccaaggcgca
     1141 ggtcgcgcag ctcctgatcc gcagcgcctg gacgcgcacg ctcgcgggcg agcgccgcgt
     1201 gaagccctgg ccctgggccg acacctggcc ggttgcgcgg ctgcaggtca ccgaccgctc
     1261 gatcgacctg ttcgtgctgg cgggcgccga tggccgcgcc atcgccttcg gccccggcca
     1321 cgtgttcggg accgcgatgc cgggcgagcg cggcaacagc gtgatcggcg gccaccggga
     1381 cacgcacctc gccttcctgc gcgacctggc gccggggtcg ctgctctcgg tgcagcgccc
     1441 cgacggcatg cgccgctggt accgcgtgaa gacggcgcag gtcgtcgaca agggcgacac
     1501 ctgggtgacc cggcaggacg gcccgacgcg cctgacgctc gtcacctgct atcccttcga
     1561 cgcgctgcgc gcggggggcg accagcgcta cgtcgtcttc gcgaacgcgg tcgagcgcgc
     1621 cgccgaggcc cctgcgccgc tgccgaaagg ccagcccggc tgagttccct cccggcgtcg
     1681 atgacgccgg catttcgcca cactccccgg gacgccccgg cgcgcccgcg ccggcctcgg
     1741 tgtaaggtgt cgcttcccat ggtgacgacc cggacactga agccggaggg gcgctcggcg
     1801 cacttcaact gggccgggct cgggcgcgtc gcgccgcagg gcctggtcaa cgcgcggctg
     1861 ctcgcgcacc atgcgctgca gtggccggcg cgctgcgccc gcgccaacat caagcccgcc
     1921 gccgacgaca gccactcgag cttcgactgg gacccgggcc tcgccgcgct cgtcacgcag
     1981 ccgctgaaga cgcccgcggg cgacgtgcgc gtggggctgc ggctctcggg cctcgcgatc
     2041 ctgctggtcc gcaacggcgc gatcctcgac gtgctgcagc tgaacggcaa gcccgagcag
     2101 gtcgcgggcg tctggatcga cgcgaagctg caggcgctcg gcctgcgcgc cgcgagcgac
     2161 gtgcgcctgc cgtacggcat gcccgaccac gcggtggcgc agggcgctcg ctacgacctc
     2221 tacctgcagg agcgcgagct gggcgagctc gcgcgctggt tcgcgggcgc gtcggccgcg
     2281 ctcgaggacg cgcgcgagcg gctcgcggac ctgcgcccga gcccggtgcg cctgtggccg
     2341 caccacttcg acatcgcgag cgtcgcggcg ctcgacgcgc ccggagccga cggcgcgcgc
     2401 tcgatcggct tcgggctctc gcccggcgac gagcactacg cgcagcccta cgtctacgtc
     2461 agcccgtcgc cgagcttcga gcgcctgccc gacgcgccca agcccgggcg ctggcacacc
     2521 gacggctggt tcggcgcggt cgcgaccggc gacgcgctgc tcgagcaggt gaaccgcggg
     2581 caggcggtgc tgcgcttcat cctcgaggcc gtcgacgtcg ggcgcaagcg cttcggcgcg
     2641 tagtcactcg cccttgatcc cggcctcgcg gatcaccctg ccccactgct cgtactcccg
     2701 ccgcagcatc tccgccgcct gctccggcgt cccgctcgcg ggctccccgc cgagattggc
     2761 gaggcgcgtc cgggtctccg ggtcgttcat ggccttgacc gcctcgcggt tgaggaagtc
     2821 gacgatcgcg cgcggcgttc ccgcgggcgc gagcaggatg taccagttca ccacgacgag
     2881 ctgcggcagg ccgacctcgg ccatggtcgg cacgccgggc agcgcgtcga cgcgtttgtt
     2941 gtcgagcacg gcgagggcgc gcatgcgtcc gtccttcacc atcgccgcga ccgaggacga
     3001 cgcgacgatg acgacgtcga cctcgtgcgc gagcgcgcct gtcagggcga tggtggcgct
     3061 cttgtagggg acgtgcagga tgtcgatctt cgtcatcgac ttgagcagct cgcccgcgag
     3121 gtggttcgac gagccgacgc cgcccgaggc gtaggtgagc ttgccgggac tcctgcgcgc
     3181 gagcgcgatc agctcgtcca tcgtcttcgc gggcaccgaa ggatgcacca ccagcacgtt
     3241 cgggatcgcg gtgaggagcg cgatcggcgc gaggtcgcgc agcgggtcgt aattcacctt
     3301 gttgaggtgc gggttgatca cgagcggcgg cgcggccatg agcagcgtgt agccgtcggg
     3361 cggcgccttc gccacgagct cgtgcgcgat gttgccgccc gcgccgagac gcggctcggg
     3421 cacgagctgc tgcccgatcg ccggcgacat cttcgccgag agcagccgcg ccaccgcgtc
     3481 ggtcccgccc gcgaaaggca ggatcacgcg gatcggcctg gcgggatatg attgcgaaaa
     3541 cgccggcgcc gagatcgcca gcagcgccgc gagcgccatt ccccatcgag cggacgatcc
     3601 cattgaggcc tccgagaaga aacagcggca ttgtagcgac cgcccgcgca cgttgccggg
     3661 ggctccgccc ggaccacgcc cgaaaccgat cggcgccgcg cctgtctgtg cttgactcat
     3721 gcccatgccg tacgagaccg gcctcgcgcg cggggagctc ttctcgatga gcggcgcggg
     3781 cgcgttccgc gcgcgcctcg ccgcgctcac gccggcgatg ctgcgcggcc tgaatcgcgg
     3841 catcgagaag gagagcctgc gcgtcgagcc cgacggcgtg ctcgcgctca cgccgcaccc
     3901 gcgcgctttg ggatccgcgc tcacgcatcc gcacatcacg accgacttct ccgagtcgct
     3961 gatcgagctg atcaccggcg tgcacaccga ggtcgagtcc tgcctcgatg agctcgagcg
     4021 catccaccag ttcgtctacc gccatatcgg cgacgagatg ctgtgggcgg cgagcatgcc
     4081 gtgctgcctg ccgaacgagc agcagatccc gatcgcgcgc tacggcacct cgaacgtggc
     4141 gcaggcgaag accgtctacc gcgtcggcct cacgcaccgc tacggcaagc gcatgcagat
     4201 gatcgccggg ctgcactaca actggtcgat gcccgagagc gcgtggaagc tgccggggtt
     4261 cccggacgcg agcaccgggt acttcgcgct catccgcaac ttcaggcgca acgcgtggct
     4321 gctgatgctg ctcttcggcg cgtcgcccgc cgtgtgctcg accttcgtcg agggacggcc
     4381 gcacggcctg cagcccttcg gtcccgggac cctgtacctg ccgcacgcga cgtcgctgcg
     4441 catggggcgc ctcggctacc tgtccgaggc gcaggacaag ctgctggtct cgtacaactc
     4501 gctcggcagc tataccgcct cgctctacga cgcgatgacc aagccgtacc ccgcgtacga
     4561 gaagatcggc atccgcgacg gcaacggcta ccgtcagctc gcgaccagcc tgctgcagat
     4621 cgagaacgag ttctacagca ccatccggcc caagcgcgtc atcaatcccg gcgagcgtcc
     4681 gctgcacgcg ctgcgcgagc gcggcgtcga gtacgtcgag gtgcgcgcga tggacctcga
     4741 tcccttctcg ccggtcggga tccgcgcgca gaccgtgcgc tttctcgacg tgttcctcct
     4801 gcactgcctg ctcgccgaca gcccgctcga cacggccgag gagatcgagt ccgtcggccg
     4861 caacaagcag cgcatcgccg cgcgcgggcg cgagcccggg ctcacgctcg accgcggcgg
     4921 ccaggtggtc accgtgcgcg actggggcgc cgacatcgtg cgcgagtgcg agcccatcgc
     4981 cgacgccctc gacgccgcgc acggcggcag cgcccaccgc gacgccctcg ccgccgccac
     5041 cgagtccctc gagaaccccg actcgctccc gagcgcgcgc atgctcgccg agatcgaagc
     5101 gagctacgac aaggagtacc cgcccttcgc ctacgccaag tccatcgagc accggcgccg
     5161 gctgatggaa aagccgctac cgaaagaagc cgaggccgcg ttcgagcgaa tggcgaaaga
     5221 gtcgatggaa gagcagaaag cgatcgagaa ggccgacaag atcagcttcg aggagtatcg
     5281 ggagaggtat atggagcatg agaagttgag ggcttagcgg gttccgcagc gtaacgaccg
     5341 attattgatg cgcccttgac ggtcggagca atacaaatgc cactccgtag acgagtatcc
     5401 gcaggattat tccgccaatg tccaggccac cgtattgcat ctcggcccgt tcgatagccc
     5461 accagcttaa cgagcagacg cctaagatga ttgccgcaat tgcaacaggg cgaattctca
     5521 gctgggttgg aatggctgcc gcaacaaata agaggcaact caatattggg agctcccaaa
     5581 ggtcaaccga ttccgatcgg taagaactgc cccactgcca cccaaatagc acaaggatgg
     5641 ctaaag
//
LOCUS       DATDMW010000067         5762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_143146, whole genome shotgun sequence.
ACCESSION   DATDMW010000067 DATDMW010000000
VERSION     DATDMW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143146"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VI319_02870"
     CDS             <1..637
                     /locus_tag="VI319_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6862818.1"
                     /translation="MAEELRRCVKQLGFRASHLVPYCGTRNLDDPAYFPYYAEAEALG
                     VPLLCHPNSNGELVDRFDNFYKTHVLGRPTNCSAALVALVLGGVFEKFPKLEVVFFEC
                     SAEWILYWMHRMDDDWEWAKDFPQISASMKRAPSEYIRRNCWVTCEADEADLARPIAE
                     LGEDRILMASDYPHFDSEYPHTVSSIRERKDLTARQKDKILGENAARLLRL"
     gene            612..1667
                     /locus_tag="VI319_02875"
     CDS             612..1667
                     /locus_tag="VI319_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6862819.1"
                     /translation="MRLACFACSLLLAATASAVEPAPGVAAVAAYAGPDRAARLAAGA
                     KQEGELMVYSSLVQEDQRKLAADFKQRYGVALRFWRGSQANILQRVLNEARGGRFDFD
                     AIETNAPQVEVLAREQLLQKVPSPLLDQELLPETVPPHGEWAPDRLNLLVYAYNTRAV
                     AASEVPKRWEDLLDPRWKRRIGMESTNVEWFAAVVEALGEKTGLDLFRRLGDNGVAVR
                     TGHNHSAVLVAAGEIPLMMGIYSHDAERMKAKSAPVDWFVLPPAIVLPSAVAVSRRAP
                     HPHAAVLFYDYMLSDGQRFYTEVHRIPANKSYDTPVRRLVEARRPVRIVDAREAIDDY
                     DRWQALYKRLIVDRSQN"
     gene            1687..3081
                     /locus_tag="VI319_02880"
     CDS             1687..3081
                     /locus_tag="VI319_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6862820.1"
                     /translation="MANEIDASARLLARLERLPFSGWHKSFLTVAFFGTLFDAADFAL
                     FGAALPVIAKEFHLGPAQAGQLATVGLIGAFLGALFWGTISDYIGRRTAFKWTIGIFA
                     AFTGAIAASWSVASLAVFRFLSNFGLGGEVPVASTLSSEFMPSKIRGRATGTMMAAFP
                     AGLVLAAGLALAVLPNYGWRTLFALGVLPALVLFFVRRNVPESVRYLVSKGRIADAEK
                     TVGEIEQRALGGAAAPAAATSPEIAAAPQAARAAESKVTVLELLRQGRATRTLLLWAV
                     SFCFLWSSNGILFMLPTILTQRGMPLSQALGLVLVQAIGGVFGYVACAFLIDRYGRRP
                     ILFLYFFVGAGFHLWFALATGAWMYAAIAMVGWVNPGVYGPSTVYVAELYPTHLRATA
                     VGWYFGIGRIGSFLAPTVVGLMIAGGVGQYVLHTFALTYLIAAIAIWLVGVETKGMVL
                     EQITGLDVAAAGAK"
     gene            complement(3094..3867)
                     /locus_tag="VI319_02885"
     CDS             complement(3094..3867)
                     /locus_tag="VI319_02885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6862821.1"
                     /translation="MSLGGKTAWITGAGSGIGQSAAVELARAGAAVVVSGRRADALAE
                     TVALVQKAGGKADAVALDVADREAVARAGEDILRRHGRVDILVNSAGLNVPNRFFKNV
                     TAKDWDHVMGINLHGAMYCMLAVLPAMRERRDGLVINVSSWFGRYPSYMGGPAYNASK
                     RALATITEQVNLEEGHRGVRGCVIFPGEVATPILKSRPKPVSAEDQARMLQSADLGRL
                     IRFVAETPAHVCLNEIVISPTWNRLILGGADISLAPKPD"
     gene            complement(3864..4313)
                     /locus_tag="VI319_02890"
     CDS             complement(3864..4313)
                     /locus_tag="VI319_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY6862822.1"
                     /translation="MAEIAQGTGQVVYVGFWKRFAAMWIDTLILTVVIALIVLPIYGS
                     QYPALKAQGQTMWIDNVINVGAILATILLWRFRGATPGKMLFSAKIVDANTLAAPSMG
                     KLVGRFFAYLVSMIPLGLGFLWIAFDARKQGWHDKLAGTVVIEEPDA"
     gene            4405..5451
                     /locus_tag="VI319_02895"
     CDS             4405..5451
                     /locus_tag="VI319_02895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY6862823.1"
                     /translation="MKAVLCKAWGPPGSLVVEDAPSPAPGPGRVLVAVRAAGVNFPDV
                     LIVQGKYQLKPELPFSPGNEIAGVVKRVGEGVRGFMPGDPVIALIGWGGFAEEVVVDE
                     KKLAPMPAGIDYTVAAAFGVTYATSYHALKDRARLAAGETLLVLGAAGGVGLAAVELG
                     KLLGARVIAAASSEAKLAACRERGADAVIDYEREDLREAIRALTDGKGVDVVYDPVGG
                     RWAEPAVRGLAWRGRYLVVGFAAGDIPKIPLNLVLLKGSAIVGVYWGDFTRREPRAAA
                     ENFEQLIAWLRAGKIRPLVSATYPLERAAQALEDLMSRKVRGKAVLVTGAPPPPADTG
                     ETTVRLSARPTGRP"
     gene            complement(5411..>5762)
                     /locus_tag="VI319_02900"
     CDS             complement(5411..>5762)
                     /locus_tag="VI319_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862824.1"
                     /translation="ALAGGRGGHFGRSSSPFAVGHSGSGSVARPGFVHSRPFFHHGGR
                     VIVGSTIFIGAAAYPYYYYPYYPPAYLPPAYEEEPPVTYIEQSDQVRYYCPDSRAYYP
                     SVTACPSAWLKVVP"
BASE COUNT          824 a         2017 c         2039 g          882 t
ORIGIN      
        1 gatggccgag gagctgcgcc gctgcgtgaa gcagctcggc ttccgcgcct cgcacctcgt
       61 gccctactgc ggcacgcgca acctggacga tcccgcgtac ttcccttatt acgccgaggc
      121 cgaggcgctc ggcgtgccgc tgctgtgcca cccgaactcg aacggcgagc tcgtcgaccg
      181 cttcgacaat ttctacaaga cgcacgtgct cgggcggccc acgaactgca gcgccgcgct
      241 ggtcgcgctc gtgctcggcg gcgtgttcga gaagtttccg aagctggagg tcgtgttctt
      301 cgagtgcagc gcggagtgga tcctgtactg gatgcaccgc atggacgacg actgggaatg
      361 ggcgaaggac ttcccgcaga tctcggcgtc gatgaagcgc gcgccctcgg aatacatccg
      421 ccgcaattgc tgggtgacct gcgaggccga cgaggccgat ctcgcgcggc cgatcgccga
      481 gctcggcgag gaccgcatcc tgatggcgag cgactacccg cacttcgact cggaataccc
      541 gcacacggtg tcctccatcc gtgagcgcaa ggacctcacc gcgcgccaga aggacaagat
      601 cctcggcgaa aatgcggctc gcctgcttcg cctgtagcct gctcctggcc gcgaccgcgt
      661 cggcggtcga gcccgcgccc ggcgtcgcgg cggtcgccgc gtacgccgga cccgaccgcg
      721 cggcgcgcct ggccgcgggc gcgaagcagg aaggcgagct gatggtctat tcctcgctcg
      781 tgcaggagga ccagcgcaag ctcgccgccg acttcaagca gcgctacggc gtcgcgctca
      841 ggttctggcg gggcagccag gcgaacatcc tgcagcgcgt cctgaacgag gcgcgcggcg
      901 gccgcttcga cttcgatgcg atcgagacca acgcgccgca ggtcgaggtg ctcgcgcgcg
      961 agcagctgct gcagaaggtc ccctcgccct tgctcgacca ggagctgctc cccgagacgg
     1021 tgccgccgca cggcgagtgg gcgcccgacc gcctgaatct cctcgtctac gcgtacaaca
     1081 cgcgcgcggt ggccgcttcc gaagtcccga agcgctggga ggacctgctc gatcccaggt
     1141 ggaagcggcg catcggcatg gagtccacca acgtcgagtg gttcgccgcc gtggtcgagg
     1201 cgctgggcga gaagaccggc ctcgatctct tccgccggct cggcgacaac ggcgtcgcgg
     1261 tgcgcaccgg gcacaaccac agcgccgtgc tggtcgccgc gggcgagatc ccgctcatga
     1321 tggggatcta cagccacgac gccgagcgca tgaaggcgaa gagcgcgccg gtcgactggt
     1381 tcgtgctgcc gcccgccatc gtgctgccgt cggcggtcgc cgtgtcgcgg cgcgcgccgc
     1441 acccgcacgc ggccgtgctg ttctacgact acatgctctc cgacgggcag cgcttctaca
     1501 ccgaggtgca ccgcatcccg gccaacaaga gctacgatac gccggtgcgc cgcctggtgg
     1561 aggcgcgccg accggtgagg atcgtcgacg cgcgcgaggc gatcgacgat tacgaccgct
     1621 ggcaggcgct gtacaagcgg ctcatcgtcg accgctcgca aaactagaaa cgaggaggag
     1681 atccgcatgg cgaacgaaat cgatgcgtcg gcgcggctgc tggcgcgcct ggagcggctg
     1741 ccgttctcgg gctggcacaa gtcgttcctc accgtggcgt tcttcggcac gctgttcgac
     1801 gccgccgact tcgcgctctt cggcgcggcg ctgcccgtga tcgcgaagga gttccacctc
     1861 gggccggcgc aggcgggcca gctcgcgacg gtcggcctga tcggcgcctt cctcggcgcg
     1921 ctcttctggg gcacgatctc cgactacatc ggccggcgca cggcgttcaa gtggacgatc
     1981 ggcatcttcg ccgcgttcac gggagcgatc gcggcatcct ggagcgtggc gtcgctcgcg
     2041 gtgttccgct tcctgtcgaa cttcggtctg ggcggcgagg tgcccgtcgc ctcgacgctg
     2101 tcctcggagt tcatgccgag caagatccgc ggccgcgcca ccggcacgat gatggcggcc
     2161 ttccccgccg ggctcgtgct cgccgcgggc ctcgcgctcg ccgtccttcc gaattacggc
     2221 tggcgcacgc tgttcgcgct cggcgtgctg cccgcgctcg tgctgttctt cgtccgccgc
     2281 aatgtgcccg agtcggtgcg ctacctcgtg agcaagggcc gcatcgccga cgccgaaaag
     2341 acggtcggcg agatcgagca gcgcgcgctc gggggcgcgg cagcgcccgc cgcggcgacc
     2401 tcgccggaga tcgcggccgc gccgcaggcc gcgcgcgccg ccgaatcgaa ggtcacggtg
     2461 ctcgagctcc tgcgccaggg ccgcgcgacg cgcacgctgc tcctgtgggc cgtgtcgttc
     2521 tgcttcctgt ggtcgtcgaa cgggatcctg ttcatgctgc cgacgatcct cacgcagcgc
     2581 ggcatgccgc tcagccaggc gctcgggctc gtgctggtgc aggcgatcgg cggggtcttc
     2641 ggctacgtcg cctgcgcctt cctcatcgac cgctacgggc ggcgcccgat cctgttcctg
     2701 tacttcttcg tcggcgcggg cttccacctg tggttcgcgc tcgcgaccgg cgcctggatg
     2761 tacgcggcga tcgcgatggt cggctgggtc aaccccggcg tgtacggacc gagcacggtc
     2821 tacgtcgccg agctctaccc cacgcacctg cgcgcgaccg cggtcggctg gtacttcggc
     2881 atcggccgca tcggctcgtt cctcgcgccg acggtggtcg gtctgatgat cgcgggcggc
     2941 gtggggcagt acgtcctgca caccttcgcg ctcacgtacc tcatcgccgc gatcgcgatc
     3001 tggctggtcg gcgtcgagac gaagggaatg gtgctcgagc agatcaccgg cctggacgtc
     3061 gcggccgccg gagcgaagta gcctcggttc ggctcagtcc ggcttcggcg cgagcgagat
     3121 atcggcgccg ccgaggatca gccggttcca ggtcggcgag atcacgatct cgttcaggca
     3181 gacgtgcgcc ggcgtctcgg cgacgaagcg gatgagccgc ccgaggtcgg ccgactgcag
     3241 catgcgcgcc tggtcctcgg ccgacacggg cttcgggcgc gacttcagga tcggcgtcgc
     3301 cacctcgccc gggaagatga cgcagccgcg gacgccgcgg tgcccctcct cgaggttgac
     3361 ctgctcggtg atcgtggcga gcgcgcgctt gctcgcgttg taggcgggcc cgcccatgta
     3421 gctcgggtag cggccgaacc acgacgacac gttgatcacg agcccgtcgc ggcgctcgcg
     3481 catcgcgggc agcacggcga gcatgcagta catcgccccg tgcaggttga tgcccatgac
     3541 gtggtcccag tccttcgccg tcacgttctt gaagaagcgg ttcgggacgt tgaggccggc
     3601 gctgttcacg aggatgtcga cgcgaccgtg acggcgcagg atgtcctcac ccgcgcgcgc
     3661 gaccgcctcg cggtcggcga cgtcgagcgc gaccgcgtcc gccttgccgc ccgccttctg
     3721 caccagcgcg acggtctcgg cgagcgcatc ggcgcggcga cccgacacga ccaccgccgc
     3781 gcccgcgcgc gcgagctcga ccgcggccga ctggccgatc ccgctgcccg cgccggtgat
     3841 ccaggcggtc ttgcccccga gactcaagcg tcgggctcct cgatcacgac cgtgccggcg
     3901 agcttgtcgt gccagccctg cttgcgcgca tcgaatgcga tccacaggaa tccgaggccc
     3961 agcgggatca tcgacacgag gtaggcgaag aagcggccga cgagcttgcc catcgacggg
     4021 gcggcgagcg tgttcgcgtc cacgatcttc gccgagaaca gcatcttgcc cggcgtcgcg
     4081 ccgcggaagc gccagagcag gatcgtggcg aggatcgccc cgacattgat gacgttgtcg
     4141 atccacatcg tctggccctg tgctttcaac gcggggtatt ggctgccgta gatcggcagc
     4201 acgatcagcg cgatcacgac ggtgaggatc agcgtgtcga tccacatcgc cgcgaagcgc
     4261 ttccagaatc cgacgtacac gacttgcccc gttccctgcg cgatttccgc catgacgtct
     4321 ccctgaggtc gttgcgggtc gcacgatggt agcgcctccc gccgggcggc ggcaatgccg
     4381 ccgtcggcgt tatcatcgcc cgatatgaaa gccgtgctgt gcaaggcctg gggaccgccg
     4441 ggcagcctgg tggtcgagga cgcgccctcg cccgcgccgg gtccgggccg cgtgctcgtc
     4501 gccgtgcgcg cggcgggcgt gaacttcccc gacgtgctca tcgtccaggg caagtaccag
     4561 ctcaagcccg agctgccgtt ctcgcccggc aacgagatcg cgggcgtggt gaaacgcgtc
     4621 ggcgaaggcg tgcgcggctt catgcccggc gatccggtga tcgcgctgat cggctggggc
     4681 ggattcgccg aggaggtggt cgtcgacgag aagaagctcg cgccgatgcc ggccggcatc
     4741 gactacaccg tcgccgccgc cttcggcgtg acgtacgcga cctcgtacca tgcgctcaaa
     4801 gaccgcgcgc ggctcgcggc gggcgagacg ctgctcgtgc tcggcgccgc aggcggcgtc
     4861 gggctcgccg cggtcgagct cggcaagctg ctcggcgcgc gcgtgatcgc ggccgcgtcg
     4921 agcgaggcca agctggcggc ctgccgcgag cgcggcgccg acgccgtgat cgactacgag
     4981 cgcgaggacc ttcgcgaggc gatccgcgcg ctcaccgacg gcaagggcgt cgacgtcgtc
     5041 tacgacccgg tcggcggccg ctgggccgag cccgcggtgc gcggcctggc gtggagaggg
     5101 cgctacctcg tggtcggctt cgccgccggc gacatcccga agatcccgct gaacctcgtg
     5161 ctgctgaaag gcagcgcgat cgtcggcgtg tactggggcg atttcacgcg tcgcgagccg
     5221 cgcgcggccg ccgagaattt cgagcagctg atcgcgtggc tgcgcgccgg gaagatccgc
     5281 ccgctggtga gcgcgaccta tccgctcgag cgcgcggcgc aggcgctcga ggacctgatg
     5341 agccgcaagg tgcgcggcaa ggcggtgctg gtgacgggcg cgccgccgcc ccccgcggac
     5401 accggcgaga ctacggtacg actttcagcc aggccgacgg gcaggccgtg acgctcgggt
     5461 agtaggcgcg cgagtcgggg cagtagtagc gcacctggtc ggactgctcg atgtaagtga
     5521 ccggcggctc ctcctcgtac gcgggcggga ggtaggccgg cggatagtac gggtaatagt
     5581 agtagggata ggccgccgcg ccgatgaaga tcgtcgagcc cacgatcacc cggccgccgt
     5641 ggtggaagaa cggccgcgaa tgcacgaagc cgggccgcgc gacggacccg gaccccgagt
     5701 gcccgacggc gaacggcgag gaggagcggc cgaagtggcc gccgcgaccg cccgcgagcg
     5761 cc
//
LOCUS       DATDMW010000068         5900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2259839, whole genome shotgun sequence.
ACCESSION   DATDMW010000068 DATDMW010000000
VERSION     DATDMW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2259839"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..358
                     /locus_tag="VI319_02905"
     CDS             56..358
                     /locus_tag="VI319_02905"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY6862825.1"
                     /translation="MIVTVFRSRLRPEAQEEYSRWAARMSELAKKMPGHISHKWFTAP
                     DGERVTIVEFENEEAQRAWATHAEHMDAKKKGRADFYLEYRVQVCNVVRDSGFEKK"
     gene            607..1497
                     /locus_tag="VI319_02910"
     CDS             607..1497
                     /locus_tag="VI319_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862826.1"
                     /translation="MATIVHEGIERRTYPRRAHDRTTMALRDNVGSAPVAVSDTDGMR
                     VSWGGIWGGVLVGMGILVLLTALGLAIGATAVDPTDTEAQKIGIGAAIWAGITLLIAL
                     YVAGHVSTRIGAIFDRTTSTYEGFLVWVVSILFMAFFAASGIGLVASGAFKLVGGATQ
                     AIGTVVSGGNLSDLSQGNVDQIVQRLRDPQTSQMLAGATGMDAQKVQQDLSQLADKAA
                     QAKDNPSQAAADVRQGVQQMIQQAKDDGTLARAAERAQEAAKKTAWITFIALILSLLA
                     ALLGAWSGRRGAAVTAGREA"
     gene            complement(1518..2717)
                     /locus_tag="VI319_02915"
     CDS             complement(1518..2717)
                     /locus_tag="VI319_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY6862827.1"
                     /translation="MKLGIDRLLEDAALRKPLAGRRVALLAHPASVTSDLTHSLDALA
                     RAGVRLTAAFGPQHGLRGDKQDNMVESPDTTDPIHGIPVFSLYGKVRRPTPQMMDAFD
                     VLLVDLQDLGTRVYTFVTTLRYVLEAGAQHKKSVWVLDRPNPVGRPVEGTKLQPGWES
                     FVGAGAMPMRHGLTLGELARWFVKTLRLDVEVRVIEMEGWKPDAAPGHGWPLGERAWV
                     NPSPNAPNLAMARCYPGTVMLEGTTLSEGRGTTRPLELFGAPDIDPRALITEMRKLAP
                     QWLNGCALRECWFEPTFHKHAGKLCAGVQIHAESPVYGHDAFRPWRLMALAFKALKWI
                     ARDYELWRDFPYEYETRLAIDVINGGPALKQWVDDRSATPADLDALAAPDEKAWRDER
                     ESFLIYR"
     gene            complement(2714..3265)
                     /locus_tag="VI319_02920"
     CDS             complement(2714..3265)
                     /locus_tag="VI319_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEY6862828.1"
                     /translation="MPAERAVALACHPDSRAGAVRGVRARVLRSAGGALAVAFTIEAD
                     PRRLRIPPPAAPRFADELWKHTCCEVFVARRGASAYHEFNLSPSGEWAAYAFRAYRER
                     ADLDIAALEPGIVVERSAAALELEATLPLGRLSPGHASAPLALGLSAVIEDAGGALSY
                     WALAHPPGRPDFHHTEAFALDLE"
     gene            3326..3868
                     /locus_tag="VI319_02925"
     CDS             3326..3868
                     /locus_tag="VI319_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862829.1"
                     /translation="MRLAAIALALASIALAAPARAADVDYHLTGKPGVVTLVNLHPDE
                     KAKRLYSVNYQLDSLMPACTKVNILSVTNKHMEFEAGGTKYEYIFHDTMKDPVAKHLD
                     KYFGTACPRKKIDSLTGVNRQGFKEGRILPGMTKDAVILAAGYPPEHATPSLESTTWK
                     YWRNKFATHSVVFQGGKVKP"
     gene            complement(3865..4488)
                     /locus_tag="VI319_02930"
     CDS             complement(3865..4488)
                     /locus_tag="VI319_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY6862830.1"
                     /translation="MTPAAPLPTMHGVMSVKYFFRDVAKRIAWTAKHPVFELRLAWYR
                     FNPFPWIVAAIVGGMAVAISFAIDAMGDLRAERQEVLCLAKNVYYEARGESATGQIAV
                     AEVTMNRLASGRYADSVCGVVYQKNWDPIRKRYVGAFSWTELDELPPLAGDEWLRALT
                     IADAVYRGNEPSVLDDRTMFYHATYIKPDWAHGKRQTARIGGHVFYR"
     gene            4618..5088
                     /locus_tag="VI319_02935"
     CDS             4618..5088
                     /locus_tag="VI319_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862831.1"
                     /translation="MRLFAIWLVLYVIRSGPGLWVMNARESDATGMALVLVVVAVMLL
                     VAALLWLFPLSVASALMPRSALEQRGALPIEDLQRCGFLLLGLWVLATSIPGVLRYAF
                     IYFLTSQRGNAAAFAPEVPAAFAGLIAETLIGIWLVFGAKGLVGLVRWARDAGH"
     gene            complement(5085..5414)
                     /locus_tag="VI319_02940"
                     /pseudo
     CDS             complement(5085..5414)
                     /locus_tag="VI319_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5430..>5900)
                     /locus_tag="VI319_02945"
     CDS             complement(5430..>5900)
                     /locus_tag="VI319_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEY6862832.1"
                     /translation="RGARARELILDGETIALRADGTPYPFQETMRRFGRRLDADELRA
                     AMPLSVYCFDCLLADGEDLTAQPARERFEALLRVLPPSSVIPRIVTAERADAQRFFDA
                     SLARGHEGLMAKALAAPYEAGGRGASWLKIKQANTLDLVVIAVEWGSGRRRGWL"
BASE COUNT          932 a         2004 c         2074 g          890 t
ORIGIN      
        1 ggccggccgc gggcggtaag atgtcgtccg ctcgtggcca ggcaggagag aggcgatgat
       61 cgtcacggtg ttccgctcgc ggctgaggcc cgaagcccag gaggagtact cgcgctgggc
      121 cgcgcgcatg agcgagctcg cgaagaagat gcccggacat atctcgcaca agtggttcac
      181 ggcgcccgac ggcgagcgcg tcaccatcgt cgagttcgag aacgaggagg cgcagcgggc
      241 gtgggcgacg cacgcggagc acatggacgc gaaaaagaag ggccgcgcag acttctacct
      301 cgagtaccgc gtgcaggtct gcaacgtcgt gcgcgacagc gggttcgaga agaaatagaa
      361 ccagtcaccc gagcacagtc gattgctggg aaggcgcccg gcagttattg ccgggttcct
      421 gcacctttta cgcgaacgcg ctctgcggcg cggtccgttg ggcgcatgcc gccgcccgct
      481 tcggaaaacg atcggcgcgc ccgccgccgt tattgtttcg cccgcgcgcc ttctctccag
      541 gattcgcctt ccgggtccgt tacttgcatc tcgaagcggt cgagcacaac gcaccggaga
      601 caagcaatgg ctaccatcgt ccacgaaggc atcgagcgca ggacctatcc gcgccgggcc
      661 catgaccgca ccaccatggc gctgcgcgac aacgtcggca gcgcgcccgt cgcggtgagc
      721 gacaccgacg gcatgcgcgt gtcctggggc ggcatctggg gcggcgtgct ggtcggcatg
      781 ggcatcctgg tgctgctcac ggcgctcggg ctcgcgatcg gcgcgaccgc ggtcgacccg
      841 accgacaccg aggcgcagaa gatcggcatc ggcgccgcga tctgggcggg catcacgctg
      901 ctgatcgcgc tctacgtcgc tggccacgtg tcgacgcgca tcggcgcgat cttcgaccgc
      961 accacgagca cctacgaggg cttcctcgtg tgggtcgtct cgatcctgtt catggcgttc
     1021 ttcgcggcga gcggcatcgg cctggtcgcg agcggcgcgt tcaagctggt gggcggcgcg
     1081 acccaggcga tcggcacggt cgtctcgggc ggcaacctgt ccgacctcag ccagggcaac
     1141 gtcgaccaga tcgtccagcg cctgcgcgat ccgcagactt cgcagatgct cgcgggcgcg
     1201 accggcatgg acgcgcagaa agtacagcag gatctctcgc agctggccga caaggccgcg
     1261 caggcgaagg acaacccgtc gcaggccgcg gccgacgtgc gccagggcgt gcagcagatg
     1321 atccagcagg cgaaggacga cggtacgctc gcgcgcgccg ccgagcgcgc gcaggaggcg
     1381 gccaagaaga ccgcgtggat cacattcatc gcgctgatcc tgtcgctgct cgctgcgctg
     1441 ctcggcgcct ggtcgggacg gcgcggcgcg gcggtgaccg cgggacgcga ggcgtaagag
     1501 cagtcaatga cagcggccta gcggtagatc aggaagctct cccgctcgtc gcgccaggcc
     1561 ttctcgtcgg gcgcggcgag cgcgtcgagg tccgctgggg tcgcggaccg gtcgtcgacc
     1621 cattgcttca gcgccggtcc gccgttgatc acgtcgatcg cgaggcgcgt ctcgtactca
     1681 taggggaagt cgcgccacaa ttcgtagtcg cgggcgatcc atttcaacgc cttgaacgcg
     1741 agcgccatga gacgccaggg gcggaaggcg tcgtggccgt acaccggcga ttcggcgtgg
     1801 atctgcacgc cggcgcacag ctttcccgcg tgcttgtgga acgtcggctc gaaccagcat
     1861 tcgcgcagcg cgcagccgtt cagccattgc ggcgcgagct tccgcatctc ggtgatcagt
     1921 gcgcgtggat cgatatcggg cgcgccgaag agctcgagcg gccgcgtcgt gccgcggccc
     1981 tcggatagcg tcgtgccctc cagcatcacc gtcccggggt agcagcgcgc catcgcgagg
     2041 ttcggcgcgt tcgggctcgg gttgacccag gcgcgctcgc cgagcggcca gccgtggccg
     2101 ggcgcggcgt cgggtttcca tccttccatt tcgatcacgc gcacttcgac gtcgagccgg
     2161 agcgtcttca cgaaccagcg cgcgagctcg ccgagcgtca ggccgtgacg catcggcatc
     2221 gcgcccgcgc cgacgaagct ctcccagccg ggttgcagct tcgttccttc gaccgggcgg
     2281 ccgaccgggt tggggcggtc gagcacccag acggacttct tgtgctgcgc ccccgcctcg
     2341 agcacgtagc gcagcgtcgt gacgaacgtg tagacgcgcg tgccgaggtc ctggaggtcg
     2401 acgagcagca cgtcgaacgc gtccatcatc tgcggcgtcg ggcggcgcac cttgccgtac
     2461 aggctgaata cgggaatgcc gtggatcggg tccgtggtgt cgggcgactc caccatgttg
     2521 tcctgcttgt cgccgcgcag gccgtgctgc gggccgaacg cggcggtgag cctcacgccc
     2581 gcgcgcgcga gggcgtcgag cgagtgcgtg aggtcggacg tcaccgacgc cgggtgcgcg
     2641 agcagcgcga cgcgcctgcc ggcgagcggt ttgcgcagcg cggcgtcttc gagcaggcgg
     2701 tcgattccga gcttcattcg aggtcgaggg cgaacgcctc agtatgatgg aaatccggcc
     2761 gccccggcgg gtgcgcgagc gcccagtacg agagcgcgcc gccggcgtcc tcgatcaccg
     2821 ccgacagccc gagcgcgagc ggcgcgctcg cgtggccggg cgacaggcgc ccgagcggca
     2881 gcgtcgcctc gagctcgagc gcggccgcgg aacgctcgac cacgatgccc ggctcgagcg
     2941 ccgcgatgtc gaggtcggcg cgctcgcggt atgcgcggaa cgcgtacgcc gcccactcgc
     3001 ccgagggcga gaggttgaac tcgtgatacg cgctcgcgcc gcggcgcgcg acgaagacct
     3061 cgcagcacgt gtgcttccag agctcgtcgg cgaagcgcgg cgcggccggc ggcggaatgc
     3121 gcagccgccg cgggtcggct tcgatcgtga aggcgaccgc gagcgcgccg ccggcggacc
     3181 tgagcacgcg cgcgcgcacg ccgcgcacgg cgccggcgcg gctgtcggga tggcaggcga
     3241 gcgcgacggc gcgttcggcg ggcatggccg aagcttatca aaagccctac aatttccggc
     3301 gccgcaacgg aaccaggggg aaaccatgag actcgcagcc atcgccttgg cgctcgcatc
     3361 gatcgcgctc gccgcgcccg cgcgcgccgc cgacgtcgat taccacctca ccgggaagcc
     3421 cggcgtcgtc acgctggtca acctgcatcc cgacgagaag gcgaagcgcc tctactcggt
     3481 caactaccag ctcgactcgc tgatgccggc gtgcacgaag gtcaacatcc tctcggtcac
     3541 gaacaagcac atggagttcg aggccggggg cacgaagtac gaatacatct tccacgacac
     3601 gatgaaggac ccggtcgcga agcacctcga caagtacttc ggcacggcgt gcccgcgcaa
     3661 gaagatcgac tcgctcaccg gggtcaatcg gcagggattc aaggagggac ggatcctgcc
     3721 cgggatgacc aaggacgccg tgatcctcgc cgcgggctat ccgcccgagc acgccacgcc
     3781 gagcctcgag tccaccacct ggaaatactg gcgcaacaag ttcgcgacgc acagcgtcgt
     3841 gttccagggc gggaaggtca aaccctagcg gtagaacacg tgcccgccga tgcgggcggt
     3901 ctggcgcttg ccgtgcgccc agtcgggctt gatgtaggtc gcgtggtaga acatcgtgcg
     3961 atcgtcgagc accgaaggct cgttcccgcg gtacacggcg tcggcgatcg tcagcgcgcg
     4021 cagccactcg tcgccggcga gcggcggcag ctcgtcgagc tcggtccacg agaaggcccc
     4081 gacgtagcgc ttgcggatcg ggtcccagtt cttctggtag accacgccgc atacgctgtc
     4141 ggcgtagcgg cccgacgcga ggcggttcat cgtcacctcg gccaccgcga tctgaccggt
     4201 cgcggactcg ccgcgcgcct cgtagtaaac gttcttcgcg aggcacagca cttcctggcg
     4261 ctcggcgcgc aggtcgccca tcgcgtcgat cgcaaacgag atcgcgaccg ccatgccgcc
     4321 cacgatcgcg gcgacgatcc acggaaacgg gttgaagcgg taccacgcga ggcgaagctc
     4381 gaacaccggg tgcttcgccg tccacgcgat ccgcttcgcg acgtctctga aaaagtattt
     4441 cacgctcatg acgccgtgca tggtcggcag cggcgccgcg ggtgtcaagg tcaaatgcct
     4501 cggtgcgcaa ccctcggatc gggctggaga aatcgcggcg aaaatcgtag agtgcgaatg
     4561 cgaacgcaac acgaaaaggt ggggtcgatg acgaacgcac aaggagttgc tgtcggtgtg
     4621 aggctcttcg cgatctggct cgtgctctac gtgatacgca gcgggccggg gctctgggtg
     4681 atgaacgccc gcgagagcga cgcgacgggg atggcgctgg tgctcgtcgt cgtcgcggtg
     4741 atgctgctgg tcgccgccct cctgtggctg tttccgctga gcgtcgcgtc ggccctgatg
     4801 ccgcgatccg cgctcgagca gcgcggcgcg ctgcctatcg aggatctcca gcgctgcgga
     4861 tttctgctcc tcggactgtg ggtgctcgcg acgtcgatcc ctggcgtcct gcgttacgcc
     4921 ttcatctatt tcctcacgtc gcagcgaggg aacgcggcag cgttcgcacc cgaggtaccg
     4981 gcggccttcg ccgggctcat cgccgagacg ctgatcggca tctggctggt gttcggcgcg
     5041 aagggtctcg tcggactcgt gcgctgggcg cgcgacgccg ggcactaggc cgcgcccgat
     5101 ccgccccggt gcagctgtga cgcgtagagc tgcctgaccg tcgcgatcgt gtcggcctgc
     5161 gccgcggtct tgtcctcgcg gtagcgcttg acgcgcgcga agcgcagcgc gagcccgcca
     5221 ggatagacgc ggctttcctg gatctcgttg tacgcgatct cgacgaccag ctcgggccgc
     5281 acgcgcacga cgggcccttc gcgcgcggtc tccagcgcga gcaggcgctc ggtctgccat
     5341 gcgagcatgg cgtcggtcat gcccttgaac gtcttcccga gcatgacgaa gccgccggcg
     5401 tcgggatcga gcgcgccgag atggagattc tagagccatc cccgccgccg gccgctgccc
     5461 cattcgacgg cgatgacgac gaggtccagc gtattggcct gcttgatctt gagccagctc
     5521 gcgccgcgcc cgcccgcctc gtagggcgcc gcgagcgcct tcgccatcag gccctcgtgt
     5581 ccgcgcgcga gcgaggcgtc gaaaaagcgc tgggcgtccg cccgctccgc agtgacgatg
     5641 cgcgggatca ccgatgaagg cggcagcacg cgcaagagcg cttcgaagcg ctcgcgcgcg
     5701 ggctgcgcgg tgagatcctc gccgtcggcg agcaggcagt cgaagcaata caccgagagc
     5761 ggcatcgcgg cgcgcagctc gtcggcgtcc agccgccgac cgaagcggcg catcgtctcc
     5821 tggaacgggt agggcgtgcc gtcggcgcgc aacgcgatcg tctcgccgtc gaggatcagc
     5881 tcgcgcgctc gagcgccgcg
//
LOCUS       DATDMW010000069        21486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1513061, whole genome shotgun sequence.
ACCESSION   DATDMW010000069 DATDMW010000000
VERSION     DATDMW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21486
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513061"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VI319_02950"
     CDS             complement(<1..1265)
                     /locus_tag="VI319_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY6862833.1"
                     /translation="MSGGPWLALAIVCGVLALVYGGWQIAWILRQPTGNDRMREIAAA
                     VQTGAMAYLNRQYTTIAIVGVILFVVIGFGLGWATAWGFALGAVLSGLTGYIGMNISV
                     RSNVRTAEAARTGLNEALTIAFRGGAITGLLVVGLGLLGVASFYAVMMASAPHGAPLS
                     DVIKPLVGLGFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIAD
                     NVGDNVGDCAGMAADLFETFAVTLIATMLLGALTVATTSDAALVFPLAIGGVSIVASI
                     IGCMFVKAIPGKKIMSALYRGLIVAAVLAAIAFIPATYSIMGPVVSQVPFDVGGGKVM
                     ITVWHLWGAAMVGLVLTALMVIITEYYTATEYSPVRHVAQASTTGHATNIIAGLGVSM
                     RSTAWPVLAVCVAIWAAYSLAGLYGIAIAA"
     gene            complement(1382..2641)
                     /locus_tag="VI319_02955"
     CDS             complement(1382..2641)
                     /locus_tag="VI319_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY6862834.1"
                     /translation="MKPRNAFYAQSGGVTAVINASAAGVIETARKYKHRIGRIYAGRD
                     GIIGALTEDLIDTSKESASAIARLKSTPGGAFGSCRYKLKGFEENRAQYERLIEVFRA
                     HDIGYFFYNGGNDSADTTLKMSQIGEKLGYPLVCVGIPKTVDNDLAVTDTCPGFGSVA
                     KYVATSIREAGFDVASMARTSTKIFVMEVMGRHAGWITAACGLAAEREGDAPQVLLFP
                     EIEFDEEKFVARVKEIVERDGWCAICVSEGLRTQDGKFVSETGLKDAFGHSQLGGAGE
                     IIAKLVQSRLGHKYHWALADYLQRAARHIASKVDFEQSYRVGAEAVKLAVSGRNAVMP
                     VIERVSNKPYRWRIGVARLADVANKEKMMPRDYITPDGFHITQKCRTYLSPLIQGEVP
                     PPFRNGLPDYVKLKNVLAPKKLQPFKV"
     gene            complement(2666..3319)
                     /gene="adk"
                     /locus_tag="VI319_02960"
     CDS             complement(2666..3319)
                     /gene="adk"
                     /locus_tag="VI319_02960"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY6862835.1"
                     /translation="MRLMLLGPPGAGKGTQADFIRQRFGIPQIATGDMLRAAVKAGTP
                     LGLEAKKVMDAGKLVSDGLIVGLVEDRLCHADCEKGCLFDGFPRTIPQAEAMRASGID
                     LDYVVEIDVDEKELLERLSGRRVHPASGRTYHVKFNPPKVPGKDDVTGEPLVQRDDDR
                     EDTVRNRIEIYKKQTRPLVEYYSNWAAQDDPRAPRYVKVSGVGSVEQVRDRIFAALK"
     gene            complement(3358..4131)
                     /gene="kdsB"
                     /locus_tag="VI319_02965"
     CDS             complement(3358..4131)
                     /gene="kdsB"
                     /locus_tag="VI319_02965"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY6862836.1"
                     /translation="MAFTVVIPARLHSSRLPRKVLAAIAGKPMVVRVAERARESGARR
                     VLVATDHADVARAVEQHGFEALMTADTHATGTDRIAEAAERLGLAADEIVVNVQGDEP
                     LVEPGLVRAVAELLGATREASIATACHPIRDAGELFNPNVVKVVLDHAGFARYFTRAP
                     VPWARDAFAASTARLPEGLPCHRHIGVYGYRVQFLRLYPKLAQSPLERFEALEQLRAL
                     WHGYAIAVALRHDAPLPGVDTAEDLERIRAAFDQVDHNR"
     gene            complement(4160..4345)
                     /locus_tag="VI319_02970"
     CDS             complement(4160..4345)
                     /locus_tag="VI319_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY6862837.1"
                     /translation="MDARLLDILVCPLCKGPLVLRKAPHELVCRGDRLGFPIRDGIPV
                     MLEQEARRLSPEEEVSD"
     gene            complement(4326..5303)
                     /gene="lpxK"
                     /locus_tag="VI319_02975"
     CDS             complement(4326..5303)
                     /gene="lpxK"
                     /locus_tag="VI319_02975"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY6862838.1"
                     /translation="MGFAERHWYRISAVSLLLFPLSLVFRAAVALRRALYRGRVLRSE
                     RLGVPVIVVGNLTVGGTGKTPLVLWLVDELRRRGLTPGIVSRGHGAGGRAPRAVRPGD
                     DPARAGDEPVLLAGRSGVPVWIGADRPAAARALLAAHPACDVIVCDDGLQHYGLVRDF
                     EIAVQDERGFGNGFLMPAGPLREPRGRPVDARVVNSDSAPEAGALRMRLAPGGLYRVG
                     DGAAMAVEALAGKRLHAVAAIGNPGRFFRTLEQMGLRPTPHAFPDHHRYAPADLDFAD
                     CDYILMTEKDAVKCRRFGRSDLIALRVDAALEPALADLIIDRIHGRAPA"
     gene            complement(5324..5740)
                     /locus_tag="VI319_02980"
     CDS             complement(5324..5740)
                     /locus_tag="VI319_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY6862839.1"
                     /translation="MKFGRTPKDDPEINLIPMIDVLLVIIIFLMLTTTYAKFAELQIN
                     LPSAAAEKQPERSNEINVVINAGGQYLVQKRAIAFRDVGSLAEELRRAGSGMKDPVVV
                     VNADNNANYQSVIRVMDAARQAGYGQVAFAVEQANR"
     gene            complement(5737..6372)
                     /locus_tag="VI319_02985"
     CDS             complement(5737..6372)
                     /locus_tag="VI319_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY6862840.1"
                     /translation="MFAIIQAAGWPIYFLLLASVIAVALIIERFIILRKEKIVPTGLL
                     DRVLAIYQREGISEDMLGTLAADSPLGQVLAAGMRNIRSSRYVMKDAIEEAGRAVSHE
                     LERFLTTLGTIATISPLMGLFGTVVGMIEIFGSQAPTGTNPIQLAHGISVALYNTGFG
                     LVIAIPAMIFYRHFRARVESFVVEMEQQAAKLVDVVHGQRFDFQPPPGAEA"
     gene            6749..8104
                     /gene="xseA"
                     /locus_tag="VI319_02990"
     CDS             6749..8104
                     /gene="xseA"
                     /locus_tag="VI319_02990"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY6862841.1"
                     /translation="MPGEPELARVSAPSRSRVLSVSALVRDVRDLLEHRYPLLWIAGE
                     VSDLTAARSGHLYFSLKDDAARVRCVMYRSRNQALDWTPREGMKVEAQALVTLYEARG
                     EFQLNVETMRPAGLGALFEAFLRLRDKLEREGLFDAASKREPPRFPRAVGVVTSLHAA
                     ALHDVLTTLARRNPALPVVVYPVPVQGEGAAERIAAAVRVAARRRDCDVLIVCRGGGS
                     IEDLQAFNDEGLARAIRACTIPVVTGIGHEIDFTIADFAADRRAATPTAAAELVSPAR
                     AELVASVAALAGRLSACLARGLDDRAQRLDFLARRLIHPEAALRARSEVLAHLVARLR
                     QASARTLDDRRWRLEQLAQRGRMRLPDVARLAARRTELLLRLQRAAHAAVERAATRCA
                     RLAENLAHLDPARVLERGYSIVERGDGAIVVDADAVSPGETVNIRFARGGAQARIERK
                     R"
     gene            8294..8782
                     /locus_tag="VI319_02995"
     CDS             8294..8782
                     /locus_tag="VI319_02995"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY6862842.1"
                     /translation="MDWVPAIARWIHFMAGVMWIGLLYYFNFVNVAAAKAATADGTAA
                     GISKHVVPRALLYFRWGAVVTWLAGATLLGANFGNAFLLHKSHLAIGIGAWLGTIMLF
                     NVWVLIWPNQKKILGMVQATDEQKNKARRVAFLASRTNTMLSIPMLWFMAAGGHGALF
                     FG"
     gene            complement(8840..9277)
                     /locus_tag="VI319_03000"
     CDS             complement(8840..9277)
                     /locus_tag="VI319_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY6862843.1"
                     /translation="MNKPAVLAAAALAAASSALAQMPVVQLVAGMYVIRAEVANTFDA
                     RATGLMFRKSLAANEGMLFVFPEAERECMWMKNTLIPLSVAFMDASGTIVSISEMQPQ
                     TETTHCAARPAKYALEMNRGWFASKGLKAGTVIRGIDKAPAAR"
     gene            complement(9348..9551)
                     /locus_tag="VI319_03005"
     CDS             complement(9348..9551)
                     /locus_tag="VI319_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862844.1"
                     /translation="MRKLISLLVAAVFAVSSGAVLAASHTGAKPMEKNGEMKNEAKKG
                     DKKPAKKAAKKADKNKKGADAPK"
     gene            complement(9699..9908)
                     /locus_tag="VI319_03010"
     CDS             complement(9699..9908)
                     /locus_tag="VI319_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862845.1"
                     /translation="MKALVVIALAATVLAASPARETHAAADQRAGADRAGTAAGAAPQ
                     AKGKPAPAPTKRKRTRTTIPPAKGK"
     gene            10054..11268
                     /locus_tag="VI319_03015"
     CDS             10054..11268
                     /locus_tag="VI319_03015"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY6862846.1"
                     /translation="MGKIKVKNPVVEMDGDEMTRIIWKMIREQLILPYLDVELKYYDL
                     GVEARDRTDDRITIEAANATRKYGVAVKCATITPDEGRVAEFKLKQMWRSPNGTIRNI
                     LDCTIFREPIICRNVPRIVPHWTKPVVVARHGFGDQYRATELKFEGPGTLKITFTPQG
                     GGAPVEREVFEAPGAGVTMAMYNLDESIKGFARACFNYALQRNYPVYLSTKNTILKVY
                     DGRFMHLFQQIFDAEFKKRFDAAGLAYEHRLIDDMVAASLKWSGGYLWACKNYDGDVL
                     SDTVAQGYGSLGLMTSVLMSPDGKAVEAEAAHGTVTRHYRLHQQGKPTSTNPIASIFA
                     WTRGLIFRGRMDGTPDVVAFAETLEKVCVDVVESGRMTRDLAVLIRPDHPFLTTEQFM
                     AAVDAELERRMH"
     gene            complement(11388..11594)
                     /locus_tag="VI319_03020"
     CDS             complement(11388..11594)
                     /locus_tag="VI319_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY6862847.1"
                     /translation="MPTGTVKWFNDAKGYGFITPDDGGEDLFAHFSAIQMSGFKTLKE
                     GQKVSFEVTQGPKGKQASNIQSSG"
     gene            11961..12269
                     /gene="clpS"
                     /locus_tag="VI319_03025"
     CDS             11961..12269
                     /gene="clpS"
                     /locus_tag="VI319_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY6862848.1"
                     /translation="MATRLREGTLLEAKKAKIKPPPLFKVILLNDDYTPMEFVVVVLQ
                     KFFSMSREKATQIMLKVHREGMGICGVYPKDIATTKVEQVVSYARKHQHPLQCVMEET
                     "
     gene            12371..14650
                     /gene="clpA"
                     /locus_tag="VI319_03030"
     CDS             12371..14650
                     /gene="clpA"
                     /locus_tag="VI319_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY6862849.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPTAAEVL
                     RACAANIEELRKALADFITAHTPTVAGTDEIDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVYFLHQQGVTRLDVVNYISHGITKTPQSKEAKSTEQEQEAA
                     GEEQQSGGALETYTLNLNAQALVGKIDPLIGRERELERVVQTLCRRRKNNPLLVGEAG
                     VGKTAIAEGLARRIVEGQVPEILSRCQVYALDMGALLAGTKYRGDFEQRLKAVLKQLV
                     DNPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSTGALKCIGATTYNEYRGVFEK
                     DHALSRRFQKIDVTEPSVDETVQILRGLKSRFEAHHGVKYSGNALTSAAELSARYIND
                     RHLPDKAIDVIDEAGAAQKILPKSKQKKVIGKVEIEEIIAKIARIPPASVSQDDRTAL
                     KNLDRDLKAVVFGQDKAIDALVAAIRTARSGLGSPTKPIGSFLFSGPTGVGKTEVARQ
                     LAYCMGIELHRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAISKKPYAVLLLDE
                     IEKAHPDIFNILLQVMDHGTLTDNNGRKADFRNIIIVMTTNAGAESLNRPVMGFTAKK
                     EVGDEMEAIKRLFTPEFRNRLDATISFRALDHEIILRVVDKFLMQLEGQLHEKKVEAI
                     FTDALREYLAKHGFDPLMGARPMSRLIQDTIRRALADELLFGKLANGGKVQIDIDPSD
                     KVALKFIEEKLPEPAPQED"
     gene            14759..15064
                     /locus_tag="VI319_03035"
     CDS             14759..15064
                     /locus_tag="VI319_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6862850.1"
                     /translation="MPRSLLPVLIILFAAPVPALAQGGHFARDLAAACASCHGTDGAS
                     VQGMPYLAGQSRTYIVERMREFKTGKRPSTVMHQIARGYTDEQIEALAAYFSAQKAQ"
     gene            15061..16332
                     /locus_tag="VI319_03040"
     CDS             15061..16332
                     /locus_tag="VI319_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6862851.1"
                     /translation="MSRARREFLLGSGALATSALLPGCAALPGRSPRRVVVVGGGYGG
                     ATAAKYLRMWSGATLEVLLVEPDAEFVSCPASNLVIGGSRAMADITRSYAGLAARGVR
                     VIRDEVTAIDADRRRVRLRNGDAIAYDRLVVSPGAELLFSQIEGYDAEAQKALPTAWK
                     AGPETLALRRQLEAMPDGGVFVITVPATPFRCPPGPYERACQVASYFKQSKPRSKVLI
                     LDANEDVASKPALFKAAWSELYKGMVEYRNNSEVKSVDARARVVRTDFDTVKGDVLNV
                     LPPMGAGAIARKAGLQTDNTKWCGVDWLTLESTTHPGIHVLGDSTLSAPAMPKSGHMA
                     NNQAKVAAAAILELLSGGSPDPAPVIANTCYSYVSDREVIHIASVHRYDAAHRTFVTV
                     PGTTGVSAARSELEALYGWAWAQNIWSDMLE"
     gene            16343..17110
                     /locus_tag="VI319_03045"
     CDS             16343..17110
                     /locus_tag="VI319_03045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEY6862852.1"
                     /translation="MKPRLEGRVAIVTGAGHGIGKAYALRLAEEGAKVVIAEIDAAAA
                     RAVAAELGAAGRQALAVATDVSDRASVEEMARRAVESFGRIDVLVNNAAIFATVPMSR
                     APFDQIDPAEWDRMMAVNLRGTWLASCAVIPQMRRQRYGKIINISSGTALKGSASRIH
                     YVTSKAGILGFTRTLAREVGGDNICVNCVAPGSTLSEENPDEGVLKMRGAAAADRALR
                     RVQKPEDLVGAVAFFASSDSDFVTGQTLVVDGGSCMH"
     gene            complement(17117..17938)
                     /locus_tag="VI319_03050"
     CDS             complement(17117..17938)
                     /locus_tag="VI319_03050"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY6862853.1"
                     /translation="MRIEPLSRVIGAEVFDVDLGALGAEDAATLRRAWLEHHVLVVRG
                     QRLSEEQLIGFGAGFGRIERARRMSPLASRPEIMVISNIRRDGEALGALPDGEMAWHF
                     DRIHQKVPNKAGVLYALEVPPRGGETRFASMCAAYDGLPERMRARLAGLTALNTFQYG
                     QTRAEDKRLSADAPSAVHPVARTIPETGRRALYVCRLMTDRILELPEGESRALLDELF
                     EQVERPEYAYQHAWRPGDVLVWDNRCVAHARNDFDERERRLLKRVTVGDEIAPAA"
     gene            complement(17947..>21486)
                     /locus_tag="VI319_03055"
     CDS             complement(17947..>21486)
                     /locus_tag="VI319_03055"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY6862854.1"
                     /translation="GRVAELVLMHRTTLVFVNTRRMAERAARHLGERLGKEAVAAHHG
                     SLAKEVRLEAEQKLRDGRLKVLVATASLELGIDIGHVDLVCQISSPHRIATLLQRVGR
                     SGHTVAGLPKGRVFPLTRDDLVECAAMVRAVKEGGLDRVAIPEKPLDVLAQQVVAESS
                     AEDWDEDALFDLFRRAWPYRNLEPRECEDVVAMLAHGYSTPRGRRAALVHYDAVHRKL
                     RARKASRMVAILSGGAIPEVFDYRVTLEPEGLFIGTLNEDFAIESLPGDVFQLGNTSW
                     RILGIGNGVVRVADAQGQPPSMPFWLGEAPSRSDEVSAAVSALRGAADAMLPAPEQPR
                     RAGELDAASDWLERDYGLSRPAAEQVAAYLAEGKRCLGVVPTAETIVLERFFDEGGGM
                     QLVLHAPLGSRINKAWGLALRKKFCQSFNFELQAAATEDGLILSLGPTHSFPLADVFR
                     YLNPKTVRETLVQAVLDSPIFETRWRWVATLALAVPRNRNGARVPAQIQRMAAEEVLA
                     AVFPDAKACFENIQGARQLPEHPLVDQALKDCLEEAMDLPKLVPYLQRVFAGEVKTLA
                     RDTPEPSVFCHEILNSAVYTFLDDAPLEERRTRAVYTRRATAARSADDLGALDPAAIA
                     RVREEAWPSAGTADELHDALMLSGFLRADEAAPGWRALLDELAAAGRAVDAGGYWVAL
                     ERYDELAAVVPLGAAPRIPERLRRTWTPEDAARELVRARMEVLGPATARSLADSLGLA
                     DTALIDMALAALEAEGRVLRGHFTPGIDGPLEWCDRRLLARIHRYTLSRLRAEIEPVT
                     AAEFMRFLLHWQHVAEEQRVKGVEGLAAVVEQLDGYELAAGAWERDVLDARVSDYEPQ
                     YLDMLCLSGRVAWGRVTPGTSAKTPLRSSPIALLLREHSALWRAPAAGDPEGLGPDAR
                     AVLAGLRARGASFFHEIAGVTGLLRTQVERALGELAGAGLVTADSFSGLRALLAPSEK
                     HKRRSHRRGTPTGVDTAGRWALLVADESVPDGDRAERIARVLLARYGVVFRALLARES
                     RLPAWRELAAVYRRLEARGEIRGGRFVGGFGGEQFALPDAVGRLRAVRKLEKAGEMTA
                     LSAADPLNLVGIVTPEARVPAIAGNRVLLRDGVPIAALEGGELRRLAHSDFEDDALRT
                     LFWRRSGEIGFVPRALSPARRRRALAEQADRLG"
BASE COUNT         3424 a         7463 c         7331 g         3268 t
ORIGIN      
        1 gccgcgatcg cgatgccata gaggcccgcg agcgagtacg cggcccagat cgccacgcat
       61 acggccagca ccggccacgc ggtggagcgc atcgagacgc cgaggccggc gatgatgttg
      121 gtcgcgtggc ccgtggtcga cgcctgcgcg acgtgacgca cgggcgagta ctcggtcgcc
      181 gtgtagtact cggtgatgat gaccatcagc gccgtgagca ccagcccgac catcgccgcg
      241 ccccagaggt gccagaccgt gatcatcacc ttgccgccgc cgacgtcgaa cggcacctgg
      301 ctcaccaccg gccccatgat cgagtacgtg gccgggatga acgcgatcgc ggcgagcacg
      361 gccgcgacga tcagcccgcg gtacagcgcg ctcatgatct tcttcccggg gatggccttc
      421 acgaacatgc agccgatgat cgatgccacg atcgacacgc cgccgatcgc gagcgggaac
      481 accagcgccg cgtccgaggt cgtggcgacc gtcagcgcgc cgagcagcat cgtggcgatc
      541 agcgtcacgg cgaacgtctc gaacaggtcc gccgccatgc cggcgcagtc gccgacgttg
      601 tcgcccacgt tgtcggcgat caccgcgggg ttgcgcggat cgtcctcggg gatgccggcc
      661 tccaccttgc cgacgaggtc ggcgccgacg tcggcgccct tggtgaagat gccgccgccg
      721 agacgcgcga agatcgagat gagcgagccg ccgaagccca ggccgacgag cggcttgatg
      781 acgtcggaga gcggcgcgcc gtgcggcgcg gacgccatca tgaccgcgta gaaagacgcg
      841 acgcccagca ggcccaggcc gacgaccagc agcccggtga ttgcgccgcc gcggaacgcg
      901 atggtgagcg cctcgttcag gcccgtgcgg gcggcctcgg ccgtacgcac gttcgagcgc
      961 accgagatgt tcatgccgat gtagccggtc aggccggaga gaactgcacc gagcgcaaag
     1021 ccccacgcgg tcgcccagcc gagcccgaag ccgatcacga cgaagagaat cacgccgacg
     1081 atggcgatgg tggtgtactg ccggttcagg taggccatgg cgccggtctg caccgccgcg
     1141 gcgatctcgc gcatgcggtc gttgccggtc ggctgtcgca ggatccacgc gatctgccag
     1201 ccgccgtaga cgagagccag cacgccgcag acgatggcca acgcgagcca ggggcctcca
     1261 ctcatagact ttctcctcgg tgtcgggccc gttgcgggcg ccgctgttca gtgtttgatg
     1321 atttgtgcgc caattcgagc ggcgagatta tagcagcagc gccgccaggc tttgagcttc
     1381 atcaaacctt gaagggttgc agcttcttcg gcgcgagcac gttcttgagc ttcacgtaat
     1441 ccggcaggcc gttgcggaac ggcggaggga cttccccctg gatcaagggc gacaggtacg
     1501 tgcggcactt ctgcgtgatg tggaagccgt cgggcgtgat gtagtcgcgc ggcatcatct
     1561 tctccttgtt cgcgacatcg gcgagccggg cgacgccgat gcgccacctg tacggtttgt
     1621 tcgagacgcg ctcgatcacg ggcatgacgg cgtttcggcc cgagacggcg agcttcacgg
     1681 cctcggcgcc gacgcggtac gactgctcga agtccacctt cgacgcgatg tgccgcgcgg
     1741 cgcgctgcag gtagtcggcg agcgcccagt ggtacttgtg cccgagccgg ctctgcacca
     1801 gcttagcgat gatctcgccc gcgccgccga gctgcgaatg cccgaacgcg tccttgagcc
     1861 cggtctcgga gacgaacttc ccgtcttggg tacgcagccc ctcggagacg cagatcgcgc
     1921 accagccgtc gcgctccacg atctccttca cccgggcgac gaacttctcc tcgtcgaact
     1981 cgatctcggg aaagagcagc acctgcggcg cgtcgccctc gcgctcggcc gccaggccgc
     2041 acgccgccgt gatccagccc gcgtgccggc ccatcacctc catcacgaag atcttggtcg
     2101 acgtgcgggc catcgacgcg acgtcgaatc ccgcctcgcg gatcgaggtc gcgacgtatt
     2161 tcgcgaccga gccgaagccg gggcaggtgt cggtcacggc gagatcgttg tccacggtct
     2221 tcgggatgcc gacgcagacc agcggatagc cgagcttctc gccgatctgc gacatcttga
     2281 gcgtcgtgtc ggccgagtcg ttgccgccgt tgtagaagaa atagccgatg tcgtgcgcgc
     2341 ggaagacctc gatcagccgc tcgtactgcg cgcggttctc ctcgaagccc ttcagcttgt
     2401 agcggcacga gccgaaggcg ccgcccggcg tcgacttcag ccgcgcgatg gcagaggcgg
     2461 attccttgct cgtgtcgatc aggtcctcgg tcagcgcgcc gatgatgccg tcgcggccgg
     2521 cgtagatcct gccgatcctg tgcttgtact tgcgcgcggt ctcgatgact ccggcggcgc
     2581 tcgcattgat gacggcggtc acgccgcccg actgggcgta gaaggcgttc ctgggtttca
     2641 tcggcatggc ggagggaaaa cagatctatt tcagcgcggc gaagatgcgg tcacggacct
     2701 gctcgaccga gccgacgccg gagaccttga cgtacctcgg cgcgcgcggg tcgtcctggg
     2761 ccgcccaatt cgagtagtac tcgaccagcg gccgggtctg cttcttgtag atctcgatgc
     2821 gattcctcac cgtgtcttcg cggtcgtcat cgcgctggac caggggctcg cccgtcacgt
     2881 cgtcctttcc gggaaccttc ggcggattga atttcacgtg ataagtccgg ccggatgccg
     2941 gatgaacgcg ccggccgctg agacgctcga gaagctcctt ctcgtccacg tcgatctcga
     3001 ccacgtagtc gaggtcgatg cccgaagccc gcatcgcctc ggcctgcgga atcgtgcgcg
     3061 ggaaaccgtc gaagagacag cccttctcgc aatcggcatg acacagccga tcctcgacca
     3121 ggccgacgat gaggccgtcg gagacgagct tgcccgcgtc catgaccttc ttcgcctcga
     3181 ggccgagcgg agtgccggcc ttgaccgcgg cgcgcagcat gtcgcccgtg gcgatctgcg
     3241 gaatcccgaa ccgctgcctg atgaagtccg cctgcgtgcc tttcccggcg cctggcggtc
     3301 cgaggagcat gagtctcatg gcgtgtcagc ggtcacgggc gggcggggcc gtacaagcta
     3361 ccgattatga tcgacttggt caaacgcggc gcggattcgt tccaggtcct cggccgtgtc
     3421 gacgccgggc agcggcgcgt cgtgtcgcag cgcgacggcg atcgcgtagc cgtgccacag
     3481 cgcgcgcagc tgctcgagcg cctcgaagcg ctcgagcggc gactgagcga gcttcgggta
     3541 gaggcgcagg aactgcacgc ggtacccgta cacgccgatg tgccggtggc acggcaggcc
     3601 ttccgggagc cgcgcggtcg aggcggcgaa ggcgtcgcgc gcccacggca ccggggcgcg
     3661 ggtgaagtag cgcgcgaaac cggcgtggtc gagcacgacc ttcaccacgt tgggattgaa
     3721 cagctcgccc gcgtcgcgga tgggatggca ggcggtcgcg atcgacgctt cccgcgtcgc
     3781 gcccagcagc tcggcgacgg cgcgcaccag ccccggctcg acgagcggct cgtcgccctg
     3841 cacgttcacg acgatctcgt cggcggccag gccgaggcgc tcggccgcct cggcgatgcg
     3901 gtcggtgccg gtggcgtgcg tgtcggcggt catcagcgcc tcgaagccgt gctgctcgac
     3961 cgcgcgcgcg acgtccgcgt ggtcggtggc gacgaggacc cgccgcgcgc cgctctcccg
     4021 cgcgcgctcg gcgacgcgga ccaccatcgg cttgcccgcg atggctgcga gcaccttccg
     4081 gggcaggcgc gaggaatgca gccgggccgg gatgacgacc gtgaatgcca tgagctcggc
     4141 gccgccgcgg agcggcgcct cagtcgctca cttcttcttc gggagacagc cgccgcgctt
     4201 cctgctcgag catgacggga atgccgtcgc ggatcggaaa gcccagccgg tctccccggc
     4261 agacgagctc gtgcggcgcc ttgcgcagaa ccagcggccc tttgcacagg gggcagacca
     4321 ggatgtcaag caggcgcgcg tccatggatc ctgtcgatga tgagatcggc caatgccggc
     4381 tcgagggccg cgtcgacgcg taaggcgatg aggtcgctcc ggccgaagcg ccggcatttt
     4441 accgcatcct tctcggtcat caggatgtag tcgcagtccg cgaagtcgag gtcggccggc
     4501 gcgtagcggt gatggtcggg gaacgcatgc ggcgtcggcc gcaggcccat ctgctcgagc
     4561 gtacggaaga accgtcccgg gttgccgatg gcggcgaccg cgtgcagccg ctttcccgcc
     4621 agggcctcga cggccatcgc tgcgccgtcg cccacccggt acaggccgcc gggcgcgagg
     4681 cgcattcgca gcgcgcccgc ctccggcgcg gaatcggaat tgaccacgcg cgcgtcgacc
     4741 ggtctcccgc gcggctcgcg caacggcccc gcaggcatca ggaacccgtt gccgaagccg
     4801 cgctcgtcct gcaccgcgat ctcgaagtcg cgcaccaggc cgtagtgctg cagcccgtcg
     4861 tcgcagacga tgacgtcgca ggccggatgc gcggcgagca gcgcgcgcgc cgccgccgga
     4921 cggtcggcgc cgatccatac cggtacgccc gagcgcccgg cgagcagcac cggctcgtcg
     4981 cccgctcggg ccggatcgtc gccggggcgc accgcgcgcg gcgcccggcc ccccgcgccg
     5041 tggccgcgcg acacgatccc cggggtcagc ccgcggcggc gcagctcgtc gaccagccag
     5101 agcacgagcg gcgtcttgcc cgtgccgccc acggtgagat tgccgacgac gatcaccggc
     5161 acgcccaggc gctcgctccg aagcacgcgg ccgcggtaca gcgcacggcg cagcgccacc
     5221 gcggcgcgga acacgaggct gagcggaaag agcagcagcg agaccgcgct gatgcggtac
     5281 cagtggcgct cggcgaagcc catcggcgcg aaggccgcgc ccgctagcgg ttcgcctgct
     5341 cgaccgcgaa ggcgacctgg ccgtatccgg cctggcgcgc cgcgtccatc acgcggatca
     5401 ccgactggta attcgcgttg ttgtcggcgt tgacgacaac caccgggtcc ttcatcccgg
     5461 agccggcgcg ccgcagctcc tccgcgaggc tccccacgtc gcggaaagcg atcgcgcgct
     5521 tctggacgag gtattgcccg ccggcgttga tgacgacgtt gatctcgttg gatcgctcgg
     5581 gctgcttctc cgccgcggcg ctcggcaggt tgatctgcag ctccgcgaac ttcgcgtacg
     5641 tggtcgtcag catcaggaag atgatgatga ccagcagcac gtcgatcatc gggatcaggt
     5701 tgatctcggg atcgtccttc ggcgtgcggc cgaatttcat gcctcggcgc ccggcggcgg
     5761 ctggaagtcg aagcgttgcc cgtgcaccac gtcgaccagc ttggccgcct gctgctccat
     5821 ctccacgacg aagctctcga cgcgcgcgcg gaagtgccgg tagaagatca tggccgggat
     5881 cgcgatcacc agtccgaagc cggtgttgta gagcgcgacc gaaatgccgt gcgcgagctg
     5941 gatcgggttg gtacccgtcg gagcctgcga gccgaagatc tcgatcatgc cgacgaccgt
     6001 gccgaacagg cccatgagcg ggctgatggt cgcgatggtt ccgagcgtgg tgaggaagcg
     6061 ctccagctcg tggctcaccg cccggccggc ctcctcgatc gcgtccttca tcacgtagcg
     6121 cgagctcctg atgttgcgca tgcccgcggc cagaacctgg ccgagcggcg agtcggccgc
     6181 gagcgtgccc agcatgtcct cgctgatgcc ttcacgctgg tagatcgcga gcacgcggtc
     6241 gagcaggccg gtcggcacga tcttttcttt gcgaaggatg atgaagcgct cgatgatcag
     6301 ggctaccgcg atgaccgagg cgagcagcag gaagtagatg ggccagcctg ccgcctggat
     6361 gatggcgaac aacttgttat ctcccggaac gggcgaatcg aatcggcgga cactttagcg
     6421 tgcggggtct gaatgggcaa ggcgcaggat ttgaagagat tttttttcgg atcccgcttc
     6481 gcatcgctcg cgcgagggtc gtcgcgacgc gatgcaccag ggtttttttg ttatccacaa
     6541 ttcgtgtgga taacgttgtg aatacgcagt cagcagcgat actaagtgaa gcgggaataa
     6601 gggtttttgt cggaatgacc aaaatttgaa cggagcgcat ttatatgtag gttcaacgga
     6661 ttacatctgt ttcttcatgc gaaaccggag cgccaccgag tgcgctgcct cggaaaagtt
     6721 ccggatgtgg ataggttaga atgagtcgat gcccggagaa ccagaactgg cgcgggtttc
     6781 cgccccatcc cgctctaggg ttctcagcgt ttccgccctc gtccgcgacg tccgcgacct
     6841 gctcgagcac cgttatcccc tgctctggat cgccggcgag gtctccgatc tcaccgccgc
     6901 ccggtccggc cacctctact tcagcctcaa ggacgacgcc gcgcgcgtgc gctgcgtgat
     6961 gtaccgcagc cgcaaccagg cgctcgactg gacgccgcgc gagggcatga aagtggaagc
     7021 gcaggcgctc gtcaccctgt acgaggcacg cggggagttc cagctcaatg tcgagacgat
     7081 gcggcccgcc gggctcggcg cgctgttcga ggcgtttctc aggctgcgcg acaagctgga
     7141 aagggaagga ctgttcgacg ccgcctcgaa gcgcgagccg ccgcgatttc ctcgcgccgt
     7201 cggcgtggtg acctcgctgc acgcggccgc gctgcacgac gtgctcacca ccctcgcgcg
     7261 gcgcaatccc gcgctccccg tcgtcgtgta tccggtcccg gtgcagggcg aaggcgccgc
     7321 cgagcgcatc gcggccgcgg tgcgcgtcgc ggcccggcgg cgcgattgcg acgtgctcat
     7381 cgtctgccgc ggcggcggga gcatcgagga cctgcaggcg ttcaacgacg aaggactcgc
     7441 gcgcgccatt cgggcgtgca cgattcccgt ggtcacgggc atcggccacg agatcgactt
     7501 caccatcgcc gacttcgccg ccgatcgccg agcggcgacg ccgaccgcgg cggcggagct
     7561 ggtgagcccc gcgcgcgccg agctcgtcgc gtcggtcgcg gcgctcgccg ggcgcctctc
     7621 cgcgtgcctc gcgcgcggcc tcgacgatcg tgcgcagcgg ctcgacttcc tggcccggcg
     7681 gctgatccat cccgaggcgg cgctgcgcgc gcggtccgag gtgcttgcgc acctcgtggc
     7741 gcgccttcgg caggcttcgg cccggaccct cgacgaccgc cgctggcggc tcgagcagct
     7801 cgcgcagcgc ggccggatgc ggctgccgga tgtggcccgc ctcgccgcgc gcaggaccga
     7861 gctgctgctg cgcctgcagc gggcggcgca cgcggctgtc gagcgcgccg cgacgcgctg
     7921 cgcgcgcctc gccgagaacc tcgcgcacct cgatcccgcg cgcgtcctcg agcgcggcta
     7981 cagcatcgtc gagcgcggcg acggcgcgat cgtagtcgac gccgacgccg tgtcgcccgg
     8041 cgagacggtc aacattcgtt tcgcgcgcgg cggcgcgcag gcgcgcatcg agcgcaagcg
     8101 ctgagcgagg gtttccgcct cttgctttcg ggcgcgcttt ggacagacaa tgagcgccgc
     8161 aggccttcgg cctggatcat cgggagaacg atcatgaaga atccgcttga ttcgctgtgg
     8221 gggacgatca tctgcggcct gatcctcacg ctcatacttc acaacgtcgt gcgctacgcc
     8281 ctgggaggct gagatggact gggtacctgc gatcgcgcgc tggatacatt tcatggcagg
     8341 cgtcatgtgg atcggcctgc tctactactt caacttcgtc aacgtcgcgg cggccaaggc
     8401 ggcgacggcc gacggcaccg cggcgggcat cagcaagcac gtggtcccgc gcgctctgct
     8461 ctatttccgc tggggcgcgg tggtcacctg gctcgcgggc gccacgctgc tcggcgccaa
     8521 cttcggcaac gccttccttc tgcacaagtc gcatctcgcg atcgggatcg gcgcctggct
     8581 cggcaccatc atgctcttca acgtctgggt cctgatctgg ccgaaccaga agaagatcct
     8641 cggcatggtg caggcgaccg acgagcagaa gaacaaggcc cggcgcgtgg cgtttctcgc
     8701 ctctcgcacc aacacgatgc tgtcgatacc gatgctctgg ttcatggcgg ccggcggcca
     8761 cggcgcgttg ttcttcggat agccggaaac ggcaaaaaag aagggcggcc cagccgccct
     8821 ttttttgtcg cggcgcgacc tagcgcgcgg ccggcgcctt gtcgatgccc cggatgacgg
     8881 ttcccgcctt cagccccttg gaggcgaacc agccgcggtt catctcgagc gcgtacttgg
     8941 cgggcctggc cgcgcagtgc gtcgtctcgg tctgcggctg catttccgag atgctgacga
     9001 tggtgccgga ggcgtccatg aacgccaccg acagcgggat cagcgtgttc ttcatccaca
     9061 tgcattccct ttcggcttcc gggaacacga acagcatgcc ttcgttcgcc gcgagcgact
     9121 tgcggaacat gagaccggtc gcgcgcgcat cgaaggtatt cgcgacctcg gcccggatga
     9181 cgtacatgcc ggcgacgagc tgcacgacgg gcatctgggc cagcgcgctg gacgcggccg
     9241 cgagcgcggc ggctgcgaga acggcgggct tgttcatggg cgtgatcctg ggcgggaaag
     9301 aaaaaaggca gggcgatcgc cctgcctttc gattcgctct cggcgaatta cttcggcgcg
     9361 tcggcgccct tcttgttctt gtccgccttc ttggccgcct tcttggccgg cttcttgtcg
     9421 cctttcttcg cctcgttctt catctcgccg ttcttttcca tcggcttggc gccggtgtgg
     9481 gaagccgcga gaacggcgcc gctcgaaacc gcgaacaccg ccgcgaccag cagggagatc
     9541 agtttgcgca tgttttcctc tctgtgagat tacggttttg gaacagacca gtcccgagaa
     9601 ccagcctctc ccactaacgc cccgccgccc gtaaggttga caagggagcc ggcattgtag
     9661 tcttttcccg cccaaaatca accagctgcc ggctggccct acttcccctt cgcgggcggg
     9721 atggtggtcc tggtgcgctt gcgcttcgtc ggcgcgggcg ccggtttgcc cttcgcctgg
     9781 ggtgccgccc cggcggccgt cccggcccga tcggcgccgg cgcgctgatc cgcagcggca
     9841 tgcgtctcgc gcgcaggcga cgcggcgagc accgtcgccg ccagggcgat cacgacgaga
     9901 gctttcatat cgagcctcct gcgtcacgtt gaccgaggtc acggagggca ccccggctgc
     9961 cctataatgc gcggtttccc gagacaaaaa aagcccgcat tccgagcggc gattccccct
    10021 cttcactcgg tcccagcaga tccagcagcg cccgtgggca agatcaaggt caagaacccg
    10081 gttgtcgaga tggatggaga cgagatgacg cgcatcatct ggaagatgat ccgcgagcag
    10141 ctgatcctgc cctatctcga cgtcgagctg aagtactacg acctcggcgt cgaagcgcgc
    10201 gaccggaccg acgaccggat cacgatcgag gcggccaacg cgacgcgcaa gtacggcgtc
    10261 gcggtgaagt gcgcgaccat cacgccggac gaggggcgcg tcgcggaatt caagctgaag
    10321 cagatgtggc gaagcccgaa cggcaccatc cggaacatcc tcgactgcac gatcttccgc
    10381 gagccgatca tctgcagaaa cgttccgcgc atcgtcccgc actggaccaa gccggtggtc
    10441 gtggcgcgcc acggcttcgg cgaccagtac cgcgcgaccg agctgaaatt cgagggccca
    10501 ggcacgctga agatcacgtt cacgccgcag ggcggcggcg cgcccgtcga gcgcgaggtg
    10561 ttcgaggcgc cgggcgcggg cgtgacgatg gcgatgtaca acctggacga gtcgatcaag
    10621 ggattcgcgc gcgcctgctt caactacgcg ctgcagcgca actacccggt ctatctctcc
    10681 accaagaaca cgatcctcaa ggtctacgat gggcgcttca tgcacctgtt ccagcagatc
    10741 ttcgacgccg agttcaagaa gcggttcgac gccgcgggcc tcgcctacga gcatcgcctc
    10801 atcgacgaca tggtcgccgc gagcctgaag tggagcggcg gctacctgtg ggcctgcaag
    10861 aactacgacg gcgacgtcct gtcggacacc gtcgcgcagg gctacggctc gctcggcctg
    10921 atgacctcgg tcctgatgtc gcccgacggc aaggccgtcg aggccgaggc cgcgcacggc
    10981 acggtcacgc gccactaccg cctgcatcag cagggcaagc ccacctccac caacccgatc
    11041 gcctcgatct tcgcctggac ccggggcctg atcttccgcg gacgcatgga cggcacgccc
    11101 gacgtggtgg cgttcgccga gaccctcgag aaggtgtgcg tcgacgtggt cgagtcgggc
    11161 aggatgaccc gggacctggc ggtcctgatc cgacccgatc acccgttcct gaccacggag
    11221 cagttcatgg ccgcggtgga cgcggagctc gagcgccgca tgcactaaag aaaaacggcc
    11281 accgagagtg gccgtttttg ccgaagaaac gcccgctccg cgctgcggag cttacggcaa
    11341 gttaccgcgt ccggagcggc ggtccgcccc ggggccggcc tgtcggctca gcccgaagac
    11401 tggatgtttg aagcctgctt gcccttcggg ccctgcgtga cctcgaagct gaccttctgt
    11461 ccttccttga gcgtcttgaa gccgctcatc tgaatcgcgg aaaagtgcgc gaacaaatcc
    11521 tcgccaccgt catccggagt aatgaagcca taacccttgg catcgttgaa ccacttcacc
    11581 gttcctgttg gcatagaacc ttcccttaat ttagaacccg gggttctttc cccgaactat
    11641 gcctgttcgc ttcatcgcag cagagggatt tgacgcctgc agacgcgcat ggaggacaaa
    11701 cacaggacgc cttttacgcg ctttgcgccg tgtcgtcaag tgtcgacaga tcgtcccaac
    11761 tccttgttcc tgcggcgcgt ttaggcgcaa ccgcgacccc gcggcgacgc cgccgagcga
    11821 gcgtccgggt cgttacatta tcgcgagagc cgcctgcgcc gcaggcactg cccgaacggc
    11881 cgcgcgcacc gggacattcg cccggcgcgg cggtccaatg gtttgacgcg tttgggcatg
    11941 tggcataact ttgtcgaggt atggcgacgc gcttacgaga ggggacgctg cttgaggcga
    12001 agaaagctaa gatcaagcct ccgcccctgt tcaaggtcat cctcctgaac gacgactaca
    12061 ctccgatgga gttcgtggtg gtggtgctcc agaagttctt ctcgatgtcc cgcgaaaaag
    12121 ccacccagat catgctgaaa gtccaccgcg aaggcatggg catttgcggc gtgtacccga
    12181 aggacatcgc caccaccaag gtcgagcagg tggtctccta cgcccggaag catcagcacc
    12241 ctctccagtg cgtaatggag gaaacatagt atttcatggg ggtccggcgg acgatacttg
    12301 taaattttcg aaaatgctcc atgatttagg atgtgagctt agggcatcac cttagaaagg
    12361 cagcgataag atgattgcgc aggaactgga agtcagcctc catatggcgt tcgtggaggc
    12421 ccggcagaag cggcacgagt tcatcaccgt cgagcacctg ctgctcgcgc tgctggacaa
    12481 cccgactgcc gccgaggtgc tgcgcgcctg cgccgccaac atcgaggagc tgcgcaaggc
    12541 cctcgccgac ttcatcaccg cgcacacgcc cacggtcgcg ggcacggacg agatcgacac
    12601 ccagccgacg ctgggtttcc agcgcgtgat ccagcgcgcg atcctgcacg tgcagtcctc
    12661 gggcaagaag gaagtgaccg gggcgaacgt gctcgttgcc atcttcggcg agaaggattc
    12721 gcacgccgtc tactttctgc accagcaggg cgtgacgcgc ctcgacgtgg tgaactacat
    12781 ctcgcacggc atcacgaaga cgccgcaatc gaaggaagcg aagagcaccg agcaggagca
    12841 ggaggccgcc ggcgaggagc agcagtcggg cggcgcgctc gagacttata cgctcaacct
    12901 caacgcgcag gcgctggtcg gcaagatcga cccgctgatc ggccgcgagc gcgagctcga
    12961 gcgcgtggtg cagacgctgt gccgccgccg caagaacaac ccgctgctgg tgggcgaggc
    13021 gggcgtcggc aagaccgcga tcgccgaggg cctcgcgcgc cgcatcgtcg aagggcaggt
    13081 gcccgagatc ctgtcgcgct gccaggtgta cgcgctcgac atgggcgccc tgctcgccgg
    13141 caccaagtat cgcggcgact tcgagcagcg cctgaaggcg gtgctcaagc agctggtcga
    13201 caacccgaac gcgatcctct tcatcgacga gatccacacg ctgatcggcg ccggcgccgc
    13261 gtcgggcggg acgctcgacg cgtccaacct gctcaagccc gcgctgtcga ccggcgcgct
    13321 gaagtgcatc ggagcgacga cgtacaacga gtaccgcggc gtgttcgaga aggaccacgc
    13381 gctgtcgcgg cgcttccaga agatcgacgt caccgagccc tcggtcgacg agacggtgca
    13441 gatcctgcgc ggcctgaagt cgcgcttcga ggcgcaccac ggcgtcaagt acagcggcaa
    13501 cgcgctcacc agcgccgccg agctgtccgc gcgctacatc aacgaccggc acctgcccga
    13561 caaggcgatc gacgtgatcg acgaggcggg cgcggcgcag aagatcctgc cgaagtccaa
    13621 gcagaagaag gtgatcggca aggtcgagat cgaagagatc atcgccaaga tcgcgcgcat
    13681 ccccccggcg tcggtgtcgc aggacgaccg caccgcgctc aagaacctcg accgcgacct
    13741 gaaggcggtc gtgttcggcc aggacaaggc gatcgacgcg ctggtcgcgg cgatccgcac
    13801 cgcgcgctcg gggctcggca gcccgaccaa gccgatcggc tcgttcctgt tctcggggcc
    13861 caccggcgtc ggcaagaccg aggtcgcgcg ccagctcgcc tactgcatgg gcatcgagct
    13921 gcaccgcttc gacatgtccg agtacatgga gcggcatgcg gtctcgcgcc tgatcggcgc
    13981 gccgccggga tacgtcggct tcgaccaggg cggcctgctc accgaggcga tctcgaagaa
    14041 gccgtacgcg gtgctgctgc tcgacgagat cgagaaggcg cacccggaca tcttcaacat
    14101 cctgctgcag gtgatggacc acgggacgct gactgacaac aacgggcgca aggccgactt
    14161 ccgcaacatc atcatcgtca tgacgacgaa cgcgggcgcc gagtcgctca accgcccggt
    14221 gatgggcttc acggcgaaga aggaagtggg cgacgagatg gaggcgatca agcgcctgtt
    14281 cacgcccgag ttccgcaacc gcctcgacgc gaccatctcg ttccgcgcgc tcgatcacga
    14341 gatcatcctg cgcgtcgtcg acaagttcct catgcagctc gaaggccagc tgcacgagaa
    14401 gaaggtcgag gcgatcttca ccgacgcgct gcgcgagtac ctggcgaagc acggcttcga
    14461 cccgctcatg ggcgcccgcc cgatgtcgcg cctgatccag gacacgatcc ggcgcgcgct
    14521 cgccgacgag ctgctcttcg gcaagctcgc caacggcggc aaggtccaga tcgacatcga
    14581 cccgagcgac aaggtcgccc tcaagttcat cgaggagaag ctgcccgagc cggcgccgca
    14641 ggaagactga gcggcacgcg ccgcatccag ggccgcctcc gggcggcctt tctttttgcg
    14701 ggctgccgcc ttgacagggg cgaccccgcc gcgaaaaatg gccccggaaa gccgtcggat
    14761 gccccgctcg ctccttcctg tcctgatcat cctgttcgcc gcgccggtcc cggcgcttgc
    14821 gcagggcggc cacttcgcgc gcgacctcgc ggcggcgtgc gcgagctgcc acggcaccga
    14881 cggcgccagc gtccagggca tgccctacct cgccgggcag agccggacct atatcgtcga
    14941 gcgcatgcgc gagttcaaga ccgggaagcg gccgtcgacc gtcatgcatc agatcgcccg
    15001 cggttatacc gacgagcaga tcgaagcgct cgccgcctac ttctcggcgc agaaggccca
    15061 atgagccgcg cgcggcgcga attcctgctc ggctcgggcg cgctcgccac gagcgccctg
    15121 ctcccgggct gcgcggccct gcccggccgc agcccgcgtc gcgtcgtcgt cgtcggcggc
    15181 ggatacgggg gcgccacggc agcgaagtac ctgcgcatgt ggagcggcgc cacgctcgag
    15241 gtgctgctgg tcgagcccga cgccgagttc gtgtcgtgtc cggcgtccaa cctcgtgatc
    15301 ggcggcagcc gcgccatggc ggacatcacg cgcagctacg cgggtctcgc cgcccgcggc
    15361 gtgcgggtga tccgcgacga ggtcacggcc atcgacgccg acaggcgccg ggttcgcctc
    15421 cggaacggcg atgcgatcgc ctacgaccgt ctcgtcgtgt cgccgggagc cgagctgctc
    15481 ttctcgcaga tcgaaggcta cgacgccgag gcgcagaagg cgctgccgac ggcatggaaa
    15541 gcgggccccg agactctcgc gctgaggcgc cagctcgagg cgatgccgga cggcggggtc
    15601 ttcgtcatca cggtgccggc gacgccgttc cgctgcccgc ccgggcctta cgagcgcgcg
    15661 tgccaggtcg cgtcctactt caagcagtcg aagcctcgct cgaaagtgct gatcctggac
    15721 gccaacgagg acgtcgcgtc gaagccggcg ctgttcaagg ccgcgtggag cgagctctac
    15781 aagggcatgg tcgagtaccg caacaacagc gaggtgaaga gcgtcgatgc gcgggcgcgc
    15841 gtcgtacgca ccgacttcga cacggtcaag ggcgacgtgc tgaacgtgct gccgccgatg
    15901 ggcgccggcg ccatcgcgcg aaaggccggg ctgcagaccg acaacacgaa atggtgcggc
    15961 gtcgactggc tcaccctgga gtcgaccacg catccgggga tccacgtcct cggcgattcg
    16021 acgctctccg cgccggcgat gccgaaatcg ggtcacatgg cgaacaacca ggccaaggtc
    16081 gcggccgccg ccatcctgga gctcctgagc ggcggctcgc ccgacccggc cccggtcatc
    16141 gcgaacacct gctacagcta tgtctcggac agggaagtca tccatatcgc gtcggtgcac
    16201 cgctacgacg ccgcgcacag gactttcgtg acggttcccg ggacgaccgg cgtgtccgcc
    16261 gcgcgcagcg agctcgaggc gctgtacgga tgggcctggg cgcagaacat ctggagcgac
    16321 atgctcgagt gaaaggggcg cgatgaaacc gaggctggag ggccgcgtcg cgatcgtcac
    16381 gggcgcgggg cacgggatcg gcaaggcgta cgcgttgcgg ctcgccgagg aaggcgcgaa
    16441 ggtcgtgatc gcggagatcg acgcggccgc cgcgcgcgcg gtcgccgccg agctcggcgc
    16501 ggcgggccgg caggcgctcg ccgtggccac cgacgtctcc gaccgcgcga gcgtcgagga
    16561 gatggcgcga cgcgccgtcg aatccttcgg ccgcatcgac gtcctcgtga acaacgcggc
    16621 gatcttcgcc accgtgccca tgtcgcgcgc gccgttcgac cagatcgatc cggccgaatg
    16681 ggaccggatg atggcggtca acctgcgcgg cacctggctc gcgagctgcg ccgtcatccc
    16741 gcagatgcgc aggcagcgct acggcaagat catcaacatc agctcgggca cggcgctcaa
    16801 gggctcggcg agccgcatcc actacgtcac atcgaaggcc gggatcctgg gcttcacgcg
    16861 cacgctcgcg cgcgaggtcg gcggcgacaa catctgcgtg aactgcgtgg cccccggcag
    16921 cacgctgagc gaggagaacc ccgacgaggg cgtcctgaag atgcgcggcg cggccgccgc
    16981 ggaccgcgcg ctgcgccgcg tgcagaagcc cgaggacctg gtcggcgcgg tcgccttctt
    17041 cgcctcgtcc gacagcgact tcgtcaccgg ccagacgctg gtcgtcgacg gcggctcgtg
    17101 catgcactga gccgcgctac gcggccggcg cgatctcgtc tcccacggtc acgcgcttca
    17161 gcagccgccg ctcgcgctcg tcgaagtcgt tgcgcgcgtg cgcgacgcag cggttgtccc
    17221 agaccaggac gtcgcccggg cgccacgcgt gctggtacgc gtattcggga cgctcgacct
    17281 gctcgaaaag ctcgtcgagc agcgcccggc tctcgccctc cgggagctcg aggatgcggt
    17341 cggtcatcag ccggcagacg tagagcgccc tgcgacccgt ctcggggatc gtgcgcgcca
    17401 ccgggtgcac cgccgacggt gcatcggccg agagccgctt gtcctcggcg cgagtctggc
    17461 cgtactggaa ggtgttgagc gcggtgagcc ccgcgagcct cgcgcgcatc cgctcgggca
    17521 atccgtcgta ggccgcgcac atgctcgcga accgcgtctc gccgccgcgc ggcggcacct
    17581 cgagcgcgta gagcacgccg gccttgttgg gaaccttctg gtggatgcgg tcgaagtgcc
    17641 aggccatctc gccgtcgggc agcgcgccga gcgcctcgcc gtcgcgccgg atgttggaga
    17701 tcaccatgat ctccgggcgc gacgcgagcg ggctcatcct gcgggcgcgc tcgatgcggc
    17761 cgaaacccgc gccgaagccg atcagctgct cctcgctcag gcgctgcccg cgcacgacca
    17821 gcacgtgatg ctcgagccac gcgcggcgca gggtcgcggc gtcctccgcg ccgagcgcgc
    17881 ccaagtcgac gtcgaagacc tcggcgccga tcacccgcga gagcggctcg atgcgcacgg
    17941 gcggccctag ccgagtcggt ccgcctgctc ggcgagcgcg cgccggcggc gcgccggcga
    18001 gagcgcgcgc gggacgaacc cgatctcgcc cgagcggcgc cagaacagcg tccgcagcgc
    18061 gtcgtcctcg aagtccgagt gcgcgaggcg gcgcagctcg ccgccttcga gcgccgcgat
    18121 cggcacgccg tcgcgcagca gcacgcggtt gcccgcgatc gcgggcacgc gcgcctcggg
    18181 cgtgacgatc ccgaccaggt tgagcggatc ggccgcggac agcgccgtca tctcgccggc
    18241 cttctcgagc ttgcgcaccg cgcgaagccg gccgacggcg tcggggagcg cgaactgctc
    18301 gccgccgaag ccgccgacga agcgtccgcc gcggatctcg ccgcgggcct cgagccgccg
    18361 gtacaccgcc gcgagctcgc gccaggccgg cagccgcgat tcgcgcgcga gcagcgcccg
    18421 gaacacgacg ccgtagcgcg cgagcagcac gcgcgcgatg cgctcggcgc ggtcgccgtc
    18481 gggcacgctc tcgtccgcca cgagcagcgc ccatcgcccc gcggtatcca cgcccgtcgg
    18541 cgtgccgcgc cggtgcgagc gccgcttgtg cttctccgag ggggcgagca gcgcgcgcag
    18601 gccgctgaag ctgtccgccg tcaccaggcc ggcgcccgcg agctcgccga gcgcgcgctc
    18661 cacctgcgtg cgcagcaacc cggtgacgcc ggcgatctcg tggaagaacg aggctccgcg
    18721 cgcgcgcagc ccggcgagca ccgcgcgcgc gtccgggccc agcccttccg gatcgccggc
    18781 ggcgggcgcg cgccacaggg ccgaatgctc gcgcagcagc agcgcgatcg gcgagctgcg
    18841 cagcggcgtc ttcgccgacg tccccggcgt gacgcgtccc caggcgacgc ggccgctcag
    18901 gcacagcatg tcgaggtatt gcggctcgta gtcggagacc cgcgcgtcga ggacgtcgcg
    18961 ctcccaggcg cccgcggcga gctcgtagcc gtcgagctgc tcgaccacgg cggcgaggcc
    19021 ctcgacgcct ttcacgcgct gctcctcggc gacgtgctgc cagtggagca gaaagcgcat
    19081 gaattcggcg gccgtcaccg gctcgatctc ggcgcgcagg cggctgagcg tatagcggtg
    19141 gatgcgcgcg agcagccgtc ggtcgcacca ctcgagcggg ccgtcgatgc ccggcgtgaa
    19201 atgcccgcgc agcacgcgtc cctcggcctc gagcgcggcg agcgccatgt cgatgagcgc
    19261 ggtgtccgcg aggcccagcg aatcggcgag cgagcgcgcg gtcgccggcc cgagaacctc
    19321 catgcgcgcg cgcaccagct cgcgcgccgc gtcctcgggc gtccaggtcc ggcgcaggcg
    19381 ctcgggaatg cgcggcgcgg cgccgagggg cacgaccgcc gccagctcgt cgtagcgctc
    19441 gagcgccacc cagtagccgc cggcgtccac cgcgcgcccg gccgcggcga gctcgtcgag
    19501 gagcgcgcgc catcccgggg cggcctcgtc ggcgcggaga aagccggaca gcatcagcgc
    19561 gtcgtgcagc tcgtcggccg tgcccgcgct cggccaggcc tcctcgcgca cgcgcgcgat
    19621 ggcggccggg tcgagcgcgc ccaggtcgtc ggcgctgcgc gccgcggtcg cgcgccgggt
    19681 gtagacggcg cgcgtgcgcc gctcctcgag cggcgcgtcg tcgaggaacg tgtagacggc
    19741 cgagttcagg atctcgtggc agaacaccga cggctcgggc gtatcgcgcg cgagggtctt
    19801 cacctcgccg gcgaagaccc gctgcagata aggcacgagc ttcggcaggt ccatcgcctc
    19861 ctcgaggcag tccttcagcg cctggtcgac gagcgggtgc tcggggagct ggcgcgcgcc
    19921 ctggatgttc tcgaagcatg ccttcgcatc cgggaagacg gcggcgagca cctcttccgc
    19981 ggccatgcgc tggatctgcg cggggacgcg cgcgccgttg cgattgcgcg ggaccgcgag
    20041 cgcgagcgtc gccacccagc gccagcgcgt ctcgaagatc ggcgagtcga gcaccgcctg
    20101 gacgagcgtc tcgcgcacgg tcttcgggtt gaggtagcgg aagacgtcgg cgagcgggaa
    20161 cgagtgcgtc ggcccgagcg agaggatcag cccgtcctcg gtcgcggcgg cctgcagctc
    20221 gaagttgaag ctctggcaga atttcttgcg cagcgcgagg ccccaggcct tgttgatgcg
    20281 gctgcccaga ggcgcgtgca gcacgagctg catgccgccg ccttcgtcga agaagcgctc
    20341 gaggacgatc gtctccgcgg tcggcacgac gccgaggcag cgcttgccct cggcgagata
    20401 ggcggcgacc tgctcggccg ccgggcgcga cagcccgtag tcgcgctcga gccagtccga
    20461 ggcggcgtcg agctcgcccg cgcgccgcgg ctgctcggga gcgggcagca tcgcgtccgc
    20521 ggcgccccgc agcgcagaca ccgccgcgct cacctcgtcg gagcgcgacg gcgcctcgcc
    20581 gagccagaac ggcatcgacg gcggctgccc ctgggcgtcg gcgacgcgaa cgacgccgtt
    20641 gccgatcccg aggatgcgcc acgacgtgtt gccgagctgg aagacgtccc cgggcagcga
    20701 ctcgatcgcg aagtcctcgt tcagcgtgcc gatgaacagc ccttcgggct cgagcgtcac
    20761 gcggtagtcg aacacctcgg ggatcgcgcc gcccgacagg atcgcgacca tgcgcgaggc
    20821 cttgcgcgcg cgcagcttgc ggtgcaccgc gtcgtagtga acgagcgccg cgcgccgccc
    20881 gcgcggcgtc gagtagccgt gcgcgagcat ggcgacgacg tcctcgcatt ccctgggctc
    20941 gagattgcga taaggccacg cgcgccggaa caggtcgaag agcgcgtcct cgtcccagtc
    21001 ctcggccgac gactcggcga cgacctgctg cgccagcacg tcgagcggct tctccgggat
    21061 cgccacgcgg tcgagcccgc cctccttcac cgcgcgcacc atggccgcgc actcgaccag
    21121 gtcgtcgcgc gtcagcggga acacgcgccc cttcggcagc ccggcgaccg tgtggcccga
    21181 gcgccccacg cgctgcagca gcgtcgcgat ccggtgcggc gaggagatct ggcagacgag
    21241 gtcgacgtgt ccgatgtcga tcccgagctc gagcgacgcc gtcgcgacca gcactttcag
    21301 ccgcccgtcg cgcagcttct gctccgcctc gaggcgcacc tccttggcga ggctgccgtg
    21361 gtgcgccgcg accgcttcct tgccgagccg ctcgccgaga tggcgcgccg cgcgctccgc
    21421 catgcggcgc gtgttgacga agacgagcgt cgtgcggtgc atcagcacca gctcggcgac
    21481 gcggcc
//
LOCUS       DATDMW010000070         8635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_267815, whole genome shotgun sequence.
ACCESSION   DATDMW010000070 DATDMW010000000
VERSION     DATDMW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267815"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="VI319_03060"
     CDS             complement(<1..1816)
                     /locus_tag="VI319_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6862855.1"
                     /translation="MDAHFVQFYEGDAFLVDEVSRFIGSGLTAGDAGIVIATAPHREA
                     LGARLEPPACDPGSYIVLDAREMLSRFMLDGAPDEDRFHDSVGAIVRDAGRNGARRVR
                     AFGEMVALLWEEGKPDAAIRLEQLWNDLARRHSFSLLCAYPMRAFGRSEDAKGFMHVC
                     AEHTHVRPAETFRIPNDDVEALNRIVARLQQKAHALESEIARRRQTEEALERRERELA
                     DFVENAVEGLHKVGPDGSILWANEAELELLGYAPEEYIGHHVAEFHVDRQSCQSLLES
                     LQRGEAVYDYPAELRCKDGSVRHVQIHSNALVENGRFAYTRCFTRDVTERTLLERELK
                     AKVAQLADLDRRKDEFLAMLGHELRNPLAPIVNSIEIMKLQGDDPKRITRAREIIQRQ
                     LALMTRLVDELLDISRLTNGKIELRRETVSLAAVVERAVELARPLIDERGHRLSLDVP
                     AEPLLLDADPARLAQVLANVLHNAAKYTDPGGHIRLSARGAPGEVVVAVRDDGIGMTP
                     EMCDAAFDLFVQGPDALVQARGGLGIGLTLVRKLVELHGGSVKARSDGPGRGSDIEIR
                     LPVVVTPPAARRDPDNSRAVPLRRRRVLVVDDNADAAQS"
     gene            1936..2403
                     /locus_tag="VI319_03065"
     CDS             1936..2403
                     /locus_tag="VI319_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6862856.1"
                     /translation="MTKTLISVAAIVLLLPLAQVHGAGDVGSPAPPRVRTNPDYEAGK
                     KAVEAHDYKGALESFNRALAKEPDSADIHNYLGYSYRKTGNLDLAFKHYNEALRIDPK
                     HKATHEYIGEAYLMVNDVPKAEEHLKQLDKICFFRCEEYTELKEAIEDYKRKH"
     gene            complement(2467..2733)
                     /locus_tag="VI319_03070"
                     /pseudo
     CDS             complement(2467..2733)
                     /locus_tag="VI319_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
     gene            2977..4158
                     /locus_tag="VI319_03075"
     CDS             2977..4158
                     /locus_tag="VI319_03075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6862857.1"
                     /translation="MEIAKKNVAVLSACQALLFTNNSTVIALNGLAGYALAANKALAT
                     LPVTGWVVGAALTTFPASLLMKAVGRRAGFTLGALIGVLGAALCAWALHAASFWMLCL
                     GTTVFGVYNAFAQYYRFAAADSASPEFRPKAISLVLAGGLVGGIIGPETSKLTVGLLP
                     TLYLGGYLALIGFLALVIALLQLLEIPPVQESAAAEPARPLSAIMAQPQFVVAVVAAA
                     LGYGTMNLLMTASPLAMAVCGHPYGAAATVIGWHVIGMFGPSLFTGNLIRRFGVAAVM
                     FAGVALQFVAIGIALSGISVPHFWFALVTLGVGWNFLYIGGTTLIAGAARPSERAKTQ
                     GVNDLIIFVVLGTSSFSSGLLLETNGWRTLNLLAAPVAAVIAVTVAWFALKRRGAPVA
                     A"
     gene            4175..5161
                     /locus_tag="VI319_03080"
     CDS             4175..5161
                     /locus_tag="VI319_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6862858.1"
                     /translation="MSEALGEAAPKLDPQARAYLDAAARASLDEAAPDPSDIAAVRRA
                     YRESRRALAPATPEAGETRALGIPGPAGTVPARYYRGYGAEAGAPLPCLVYFHGGGWV
                     CGDLDTHDAVCRGIANHARCAVVSVDYRLAPEHRFPAAVDDAVAALGWVCENAAALDA
                     DPARIVVGGDSAGGNLAAVAALAARDAGGPSPALQVLFYPVTDLGLGSASYARLAQGY
                     GLTRDDMRWYRGHYLRGEEDAADWRASPLRARDVSRLPPAYIVTAGFDPLHDEGEAYA
                     RRLADAGVAVTYECFVGQIPGFLLMGGVMAAARHAVYRIGQAIRSRFFRRTM"
     gene            5195..7285
                     /locus_tag="VI319_03085"
     CDS             5195..7285
                     /locus_tag="VI319_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEY6862859.1"
                     /translation="MQAPVKKAPPRRTVVRAACPHDCPDTCALEVTVENGVAVKVEGA
                     KDHPFTAGTLCTKVARYLERTYSEERVLFPMRRVGAKGEGRFERISWDQALAEIAERF
                     AALAAEDPQQILPYDYAGHMGYVNWHAMGKRFFHRLGASLLDRTICSSAGKAGIAITL
                     GGSVGMDPERFEDAKLILIWGSNPIVSNLHLWSRVQEAKRRGAKVIAIDPYRSLTAEK
                     CSEHVAPLPGTDGALALAMMHVIVAEGLVDGEYVSRHTVGFDALKQRLADYPPQLVAG
                     ITGLPAERIVRLAREYATVKPAAIRLNYGMQRTSGGGMAARTIACLPALVGAWRDPAG
                     GVVLTTAGFFGVDTAKLGRPDLLAKSRCPKPRTINMSAIGDALNDAAPPVRALYVYNS
                     NPVAVAPESRKVIRGFSREDLFTVVHDLFLTDTCDYADIVLPATSQLEQTDVHSAYGH
                     LYFMANNAAIAPLGESLPNGEVFRRLARKMGFDDGCLYDSDDEVCRQALALAGPYGKS
                     IEWDALKREGWKRLDVPETFAPFAEGNFPTPSGKCEFYSETAKKMGIDPLPTFVPPRE
                     SVVSNPALAAKYPLAMISPPNRHFLNSSFANLPFAIAEAGAPSLDIHPDDARARGIRE
                     GDTVRIFNDRGAFTAKARVSDAARRGVVVALSVWWKKLSSDGANANEVTSQALTDLGR
                     GATFYDCLVEVQPA"
     gene            complement(7282..7899)
                     /locus_tag="VI319_03090"
     CDS             complement(7282..7899)
                     /locus_tag="VI319_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY6862860.1"
                     /translation="MQCLRRCLLGFVVFTLALTASAALGLYYAARWLDDPDPPGKSAA
                     ILILGGDSTRAIEAADLYQAGYAPKVYISAPIREPRERRLESIGVIMPSDEDTMRRVL
                     AIRGVPGSAVDLLARDMISTVEEARTTAQRLAGVPGPLLVVTSPYHVRRARMIFHSAM
                     PDRELRFIASRYEAFPADWWRDQTASRNVVLELTKMAYYVLGGRF"
     gene            7963..>8635
                     /locus_tag="VI319_03095"
     CDS             7963..>8635
                     /locus_tag="VI319_03095"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY6862861.1"
                     /translation="MGAKQRARRWPAGRDSGIQIVEEDGVRYLHIGGEAIQSGLRIDE
                     PDRLELDYVRAMMAFLLFCPRPRDVLMIGLGGGSMARFIHQRMGETRVAVVEINPEVV
                     TVARRHFHFPEDDDRLEVVVADGADVVPRRPASADVLVVDGFEDGETPEALCTQPFYD
                     AAFASLREGGVMVVNFMADARKMDAYLGRIESAFGRYPTLLMAEDRDNLIAFAAKGGP
                     ARIAWD"
BASE COUNT         1259 a         2989 c         3041 g         1346 t
ORIGIN      
        1 gcgattgcgc cgcgtccgcg ttgtcgtcga cgaccaggac ccggcggcgc cgcagcggca
       61 ccgcgcgcga attgtcgggg tcgcggcgcg cggcgggcgg cgtcaccacg acgggaagcc
      121 ggatttcgat gtcgctgccg cgtcccggcc cgtcgctgcg cgccttcacg ctgccgccgt
      181 gcagctcgac cagcttgcgc accagcgtga ggccgatccc caggcccccg cgcgcctgca
      241 cgagcgcgtc gggtccctga acgaaaagat cgaacgccgc gtcgcacatc tcgggcgtca
      301 tgccgatgcc gtcgtcgcgc acggcgacga cgacctcgcc gggagcgccg cgcgccgaaa
      361 gcctgatgtg cccgcccggg tccgtgtact tggccgcgtt gtgcaggacg ttcgcgagca
      421 cctgcgcgag gcgcgccggg tcggcgtcga gcaggagggg ctcggcgggg acgtcgagcg
      481 agagccggtg cccgcgctcg tcgatcagcg gccgggcgag ctcgaccgcg cgctcgacga
      541 cggcggcgag cgacaccgtt tccctgcgca gctcgatctt gccgttcgtg agccgagaga
      601 tgtcgagcag ctcgtcgacg agccgcgtca tcagcgcgag ctgccgctgg atgatctctc
      661 gcgcgcgcgt gatgcgcttc gggtcgtctc cctgcagctt catgatctcg atcgagttca
      721 cgatgggcgc gagcgggttg cgcagctcgt gacccagcat ggcgaggaac tcgtccttgc
      781 gccggtcgag atcggcgagc tgcgcgacct tcgccttcag ctcgcgctcg agaagggtgc
      841 gctcggtgac gtcgcgcgtg aagcatcggg tgtaggcgaa gcggccgttc tcgaccagcg
      901 cgttcgagtg gatctgcacg tggcggacgg agccgtcctt gcagcgcagc tcggccggat
      961 agtcgtacac cgcctcgccg cgctgcaggc tctcgagcag gctctggcac gattgccggt
     1021 cgacgtggaa ctcggcgaca tggtggccga tgtactcttc gggcgcgtag cccagcagct
     1081 cgagctccgc ctcgttcgcc cagaggatgc tgccgtcggg acccaccttg tgcaggccct
     1141 cgaccgcgtt ctcgacgaag tccgcgagct cgcgctcgcg gcgctcgagg gcttcctcgg
     1201 tctgccggcg ccgggcgatc tcgctctcga gcgcgtgtgc tttctgctgc agcctcgcga
     1261 cgatgcggtt cagcgcttcg acgtcgtcgt tcggaatgcg gaaggtctcc gccgggcgca
     1321 cgtgggtgtg ctcggcgcag acgtgcatga agcccttggc gtcctcgctg cggccgaagg
     1381 cgcgcatcgg gtaggcgcac aggagcgaga aggagtggcg ccgggcgagg tcgttccaca
     1441 gctgctcgag acgaatggcc gcgtccggct tgccttcctc ccagagcagg gccaccatct
     1501 cgccgaacgc gcgcacgcgc cgcgcgccgt tgcgcccggc gtcccggacg atcgcgccga
     1561 ccgagtcgtg gaagcggtcc tcgtcgggcg ccccgtcgag catgaagcgc gacagcatct
     1621 cccgggcgtc gagcacgata tacgagcccg ggtcgcacgc cggcggctcc aggcgggcgc
     1681 cgagcgcctc gcggtgcggc gcggtggcga tgacgatccc ggcatctccc gccgtgagcc
     1741 cggatccgat gaatcggctc acctcgtcga cgaggaaggc atcgccttcg tagaattgga
     1801 cgaagtgggc gtccattgag gttgtctccc aggcggcgcg gggtcacgaa ccgagtaatg
     1861 accgccgcgg cgggccgtag ttccccggaa gggcggctct cctataatgc gcaggcaaac
     1921 cccgaggaga cacccatgac gaagactctg atatcggtcg ccgcgatcgt cctgctcctg
     1981 ccgctcgcgc aggttcacgg cgcgggcgac gtcggctcgc cggcgccgcc gcgcgtgcgg
     2041 acgaaccccg actacgaggc gggcaagaag gccgtcgagg cgcacgacta caagggggcg
     2101 ctggagagct tcaaccgcgc gctcgccaag gagccggaca gcgccgacat ccacaactat
     2161 ctcggctaca gctaccgcaa gacgggcaac ctcgaccttg cgttcaagca ctacaacgag
     2221 gcgctgagga tcgatccgaa gcacaaggcg acgcacgagt acatcggcga ggcctacctc
     2281 atggtgaacg acgtgccgaa ggccgaggag catctcaagc agctcgacaa gatctgcttc
     2341 ttccgctgcg aggagtacac cgaattgaag gaggcgatcg aggactacaa gcgcaagcac
     2401 tgagccgcgg cctgcgaaaa gcaacggggc gcccgaaggc gccccgtctg ttttcagggg
     2461 ggcgtgttac ttcaggtgct tgttgaccag cttggtcatc tcgaacatcg agacggtctt
     2521 cttgccgccg aagacctcct tgagcttgtc gtcggcgttg atgttcctgc ggttcttgct
     2581 gtcctggaga cccttgcgct tgatgtattg ccagatcttc ttggtcactt cggtgcgcgg
     2641 catcggcttg gagcctacga cctcggcgag cgccgcgctc ggggtcatgg ccttcatgaa
     2701 ggcagcgttc ggttttctct tgctcgattt cttcttggcc atcgtattct ctcctctaag
     2761 taccggctgg tagggacacc gcactatggc gtacgggcaa cgcgagagtg ctgcaacccg
     2821 agggaaaagt caaccacccg agagcggttt ttcgagggaa gcagcggcga tttccgagtg
     2881 gcgcgaccgg cgttcgcggc gcgctggcgc cgcgcgttcg ctataatgac gcgacgttga
     2941 tcctgacaat cgcctgtcac acccggcggc aaccccatgg aaatcgcgaa aaaaaacgtg
     3001 gccgtgctgt ccgcgtgcca ggcgctcctg ttcacgaaca actccacggt catcgcgctc
     3061 aacgggctcg cgggctacgc gctcgctgcg aacaaggcgc tcgcgacgct gcccgtcacc
     3121 gggtgggtgg tcggcgcggc gctgacgacg ttccccgctt ctctgctcat gaaagccgtg
     3181 gggcggcgcg cgggattcac gctcggagcg ctgatcggcg tcctcggcgc ggcgctgtgc
     3241 gcctgggcgc tgcacgccgc gagcttctgg atgctgtgcc tcggaaccac cgtcttcggg
     3301 gtgtacaacg cgttcgcgca gtattaccgg ttcgccgccg ccgactcggc gagcccggag
     3361 ttcaggccca aggccatctc cctggtgctc gccggcgggc tcgtgggcgg gatcatcggc
     3421 ccggagacga gcaagctcac ggtcggcctg ctgcccacct tgtatctcgg cggctacctc
     3481 gcgctcatcg gcttcctcgc gctcgtgatc gcgctgctgc aactgctcga gattccgccg
     3541 gtccaggagt ccgccgcggc cgagccggcg cggccgctga gcgccatcat ggcgcagccg
     3601 cagttcgtcg tcgccgtcgt cgctgccgcg ctcggctacg gcacgatgaa cctgctcatg
     3661 accgcctcgc ccctcgcgat ggcggtgtgc gggcatccgt acggcgcggc cgcgacggtg
     3721 atcggctggc acgtcatcgg catgttcggt ccctcgctgt tcacgggaaa cctcatccgc
     3781 cgcttcgggg tcgccgccgt gatgttcgcc ggcgtcgcgc tgcagttcgt cgcgatcggc
     3841 atcgcgctct ccggcatcag cgtcccgcac ttctggttcg cgctggtcac gctgggcgtc
     3901 ggctggaact tcctgtacat cggcggcacg acgctgatcg cgggcgcggc gcggccctcg
     3961 gagcgcgcca agacccaggg cgtgaacgac ctgatcattt tcgtcgtgct cgggacgtcg
     4021 tcgttcagct cggggctgct gctcgagacg aacggctggc ggaccctcaa cctgctcgcg
     4081 gcgcccgtcg ccgcggtgat cgccgtgacg gtcgcctggt tcgcgctgaa gcgccgcggc
     4141 gcgccggtgg cggcctgatc cgccgggcgg cccgatgagc gaggcgctcg gcgaagcggc
     4201 gcccaagctc gacccgcagg cgcgcgcgta cctcgacgcg gcggcgcgcg cctccctcga
     4261 cgaggcggcc cccgatccgt ccgacatcgc cgcggtgcgc cgcgcgtacc gcgagtcgcg
     4321 ccgcgcgctc gcgccggcga cgcccgaggc gggggagacg cgcgcgctcg ggatcccggg
     4381 gccggcgggc acggtcccgg cgcgctacta ccgcggctac ggcgccgaag cgggcgcccc
     4441 gctgccgtgc ctcgtgtact tccacggcgg cggctgggtg tgcggcgacc tcgacacgca
     4501 cgacgcggtg tgccgcggca tcgcgaacca cgcgcgctgc gcggtcgtgt cggtcgacta
     4561 ccggctcgcg cccgagcacc gctttcccgc cgcggtcgac gacgccgtcg cggcgctcgg
     4621 gtgggtgtgc gagaacgccg ctgcgctcga cgcggatccg gcgcgcatcg tcgtcggcgg
     4681 cgacagcgcg ggcggcaatc tcgcggcggt cgcggcgctg gcggcgcgcg acgcgggagg
     4741 cccgtcgccc gcgctgcagg tgctcttcta cccggtgacc gatctcggcc tgggcagcgc
     4801 gtcgtacgcg cggctcgcgc aaggctacgg tctcacgcgc gatgacatgc gctggtaccg
     4861 cggccactac ctgcgaggcg aggaggatgc ggccgactgg cgcgcgtcgc cgctgcgcgc
     4921 gcgcgacgtg tcgcggctgc cgccggcgta tatcgtgacc gcgggcttcg atccgctgca
     4981 cgacgagggc gaggcctacg cgcggcggct cgccgacgcg ggcgtggcgg tcacgtacga
     5041 gtgcttcgtg gggcagatcc ccggcttcct gctcatgggc ggcgtgatgg cggcggcgcg
     5101 gcacgccgtc taccgcatcg gccaggcgat ccgctcgcgc ttcttccgcc gcacgatgtg
     5161 aggcgcgcag tgacggcgta gaattcgggg agccatgcag gcgcccgtca agaaagcccc
     5221 gccccggcgc accgtcgtgc gcgccgcgtg cccgcacgac tgtcccgaca cctgcgcgct
     5281 ggaggtcacg gtcgagaacg gcgtcgccgt gaaggtcgag ggcgcgaaag accatccgtt
     5341 caccgcgggg acgctgtgca ccaaggtcgc gcgctacctc gagcgcacct acagcgaaga
     5401 gcgcgtcctc ttcccgatga ggcgcgtcgg cgcgaagggc gagggccgct tcgagcgcat
     5461 ctcgtgggac caggcgctcg ccgagatcgc ggagcgattc gccgcgctcg ccgccgagga
     5521 cccgcagcag atcctgccgt acgactacgc cggccatatg ggctacgtga actggcacgc
     5581 gatgggcaag cgcttcttcc accgcctggg cgcgtcgctg ctcgaccgca cgatctgctc
     5641 ctcggccggc aaggccggga tcgcgatcac gctcggcgga tcggtcggga tggaccccga
     5701 gcgcttcgag gacgcgaagc tcatcctgat ctggggctcg aacccgattg tctcgaacct
     5761 gcacctgtgg tcgcgcgtgc aggaggcgaa gcgccgcggc gcgaaggtga tcgcgatcga
     5821 cccgtaccgc agcctcaccg ccgagaagtg cagcgagcac gtcgcgccgc tgcccgggac
     5881 cgacggcgcg ctcgcgctgg cgatgatgca cgtgatcgtg gccgaagggc tggtggacgg
     5941 ggagtacgtc tcgcgccaca ccgtcggctt cgacgcgctg aagcaaaggc tcgccgatta
     6001 cccgccgcag ctcgtcgccg ggatcacggg cctgccggcg gagaggatcg tgcggctcgc
     6061 acgcgagtac gcgacggtga agcccgcggc gatccggctc aattacggca tgcagcgcac
     6121 ctcggggggc ggcatggcgg cgcgcacgat cgcctgtctt ccggcgctgg tcggcgcatg
     6181 gcgcgatccc gcgggcggcg tcgtgctcac gaccgcgggc ttcttcggcg tcgacacggc
     6241 caagctcggg cgcccggacc tgctcgcgaa atcgcgctgc ccgaagccgc gcacgatcaa
     6301 catgagcgcg atcggcgacg cgctcaacga cgcggcgccg ccggtgcgcg cgctctacgt
     6361 ctacaactcg aatccggtcg cggtcgcgcc cgagtcgcgc aaggtgatcc gcgggttctc
     6421 gcgcgaggac ctgttcacgg tggtgcacga cctgttcctc accgatacct gcgactacgc
     6481 cgacatcgtg ctgcccgcga cgtcgcagct cgagcagacc gacgtgcaca gcgcctacgg
     6541 ccacctgtac ttcatggcga acaacgcggc gatcgcgccg ctcggcgagt cgctgccgaa
     6601 cggcgaggtg ttccgccgcc tcgcgcgcaa aatgggcttc gacgacgggt gcctctacga
     6661 cagcgacgac gaggtgtgtc gacaggcgct cgcgctcgcg ggtccgtacg gcaaaagcat
     6721 cgagtgggac gcgctcaagc gcgaaggctg gaagcggctc gacgttcccg agacgttcgc
     6781 gccgttcgcg gaaggcaact tcccgacgcc gtccggaaaa tgcgagttct acagcgagac
     6841 cgcgaagaaa atgggcatcg acccgctgcc gactttcgtg ccgccgcgcg agtcggtcgt
     6901 ctccaacccg gcgctcgccg cgaagtaccc gctcgcgatg atctcgccgc cgaaccggca
     6961 tttcctgaac tcgtcgttcg cgaacttgcc gttcgcgatc gccgaggcgg gcgcgccgag
     7021 cctcgacatc catcccgacg acgcgcgggc gagagggata cgcgagggcg acaccgtgcg
     7081 catcttcaac gaccgcggtg cgttcacggc caaggcccgc gtctccgatg cggcgcgccg
     7141 gggcgtcgtc gtcgccttgt cggtgtggtg gaagaagctc tcgagcgacg gcgcgaacgc
     7201 gaacgaggtc acctcgcagg cgctcaccga cctcggccgc ggcgcgacct tctacgactg
     7261 cctggtcgag gtgcagcccg cctagaaacg tccgcccagg acgtagtacg ccatcttcgt
     7321 cagctcgagc acgacgttgc gcgacgcggt ctggtcgcgc caccagtccg ccggaaaagc
     7381 ctcgtagcgg ctggcgatga agcgcagctc gcgatcgggc atcgcggaat ggaagatcat
     7441 gcgcgcccgg cgcacgtggt agggcgacgt gacgacgagc agaggcccgg ggacgcccgc
     7501 cagccgctgc gcggtggtcc gcgcctcctc gacggtgctg atcatgtcgc gtgccagcag
     7561 gtcgaccgcg cttccgggca ccccgcgaat ggccagcacg cgccgcatcg tgtcttcgtc
     7621 gctcggcatg atgacgccga tcgactccag ccggcgctcg cgcggctccc gtatcggcgc
     7681 gctgatgtac accttcggag cgtagcccgc ctggtacagg tccgcggcct cgatcgcccg
     7741 agtggaatcg ccgccgagaa tgaggatggc ggccgacttc ccgggaggat cgggatcgtc
     7801 cagccagcgc gcggcgtagt acaggccgag cgcggccgat gccgtcagcg cgagggtgaa
     7861 tacgacgaat ccgagcagac agcgccgcag gcattgcatc agagggccct ttccgcgatc
     7921 atcggtgcgg tcgcatttta ctgtcgcggg aggtccagcc tcgtgggagc gaagcagcgc
     7981 gcgcgccggt ggcccgccgg gcgcgattcc ggcatccaga tcgtcgagga ggacggcgtg
     8041 cgctacctcc acatcggcgg cgaggcgatc cagagcgggc tgcgcatcga cgagcccgac
     8101 cggctcgagc tcgactacgt gcgcgcgatg atggcgttcc tgctgttctg tccgcggccg
     8161 cgcgacgtgc tcatgatcgg gctgggcggc ggctcgatgg cgcgcttcat ccatcagcgg
     8221 atgggcgaga cgcgcgttgc cgtcgtcgag atcaacccgg aggtcgtcac cgtcgcccgc
     8281 cggcatttcc attttcccga ggacgacgac cgactcgagg tcgtggtggc ggacggcgcg
     8341 gacgtcgttc cgcggcgccc ggcctcggcc gatgtcctcg tggtcgacgg cttcgaggac
     8401 ggcgagacgc ccgaggcgct gtgcacgcag ccgttctacg acgccgcgtt cgcgtcgctg
     8461 cgcgaaggcg gcgtcatggt ggtcaacttc atggccgatg cgcgcaagat ggacgcctac
     8521 ctcgggcgca tcgaatcggc gttcggccgc tacccgacgc tgctcatggc cgaagaccgg
     8581 gacaatctga tcgcgttcgc ggcgaaaggc ggtccggcgc gcatcgcatg ggacg
//
LOCUS       DATDMW010000071        10013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1513339, whole genome shotgun sequence.
ACCESSION   DATDMW010000071 DATDMW010000000
VERSION     DATDMW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513339"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VI319_03100"
     CDS             complement(<1..533)
                     /locus_tag="VI319_03100"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6862862.1"
                     /translation="MKIDRREFLRSAGALVVSFSIPLDVLAQAPAQARPNPKLQQLDA
                     WLSVGGDGKVSVYTGKVELGTGVQTALAQLVADELDVPFERVHMVMGDTALCPNQGPT
                     VGSQTMMRAGPQLRQAAAEARQTLLGLASSKLDAPVERLTVADGVVAAPGGKSVSYAE
                     LIGDKKFDHAMTGKAKT"
     gene            complement(530..985)
                     /locus_tag="VI319_03105"
     CDS             complement(530..985)
                     /locus_tag="VI319_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6862863.1"
                     /translation="MTTINVNGKPHAVKAAPDTPLLYVLRNELELNGPKFGCGLAQCG
                     ACTVHIDGRPMRSCSVPVSTVKGKVVTIEGLGTPEKPHPVQKAFVEEQAAQCGYCING
                     MVMTAAAFLAKNPKPSDADIRKAMDGNLCRCGTHMRILRAVKRAAGEAA"
     gene            complement(999..2009)
                     /locus_tag="VI319_03110"
     CDS             complement(999..2009)
                     /locus_tag="VI319_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862864.1"
                     /translation="MQRIFRAAVVALAFALVSCASVAPEPPRTLTPVNLIVFPGGFNW
                     PVWVAQEKGFFARDGIEVKVTPTPSSVFQLTNLIDGRFDIAMTAVDNLIAYREGQGEV
                     PKIGPDLFAFMGGDNGFLRLVTIPSVRSFDDLRGKTLSVDALTTGYAFVLLEILERKG
                     LVHGRDYQTVSAGGVLQRFQQLMEQKHAGTLLLSPFEVQAEAKGFNRLANAIDVLGRY
                     QGLVGGARKSWADAHREAVIGYIRAYVDAVEWLYDPRNKDEAIAIFLKNLPQAGPQGA
                     ETSYRILLSPTDGFQRKAQIDMEGVRTVLQLRSKYGKPQKTLADPARYYDDSFYRAAM
                     AR"
     gene            complement(2032..2955)
                     /locus_tag="VI319_03115"
     CDS             complement(2032..2955)
                     /locus_tag="VI319_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6862865.1"
                     /translation="MIDLRDIRYLRIGTPDLESAVEYATTIVGLQLVAREGRAAYFRS
                     DKAGVRGDTRDHTLVYFEGRADDHAVGFDLRDPEALDAAAAALEKAGRPVHYGTKDEC
                     EQRRVRAFVASSDPSGNRIEIVSRPYHSGLRYHPARDAGITHFSHIGLRTSNAARDEA
                     FWTKICNARVSDWIGDAPLLRIDTIHHSLALFPSPHPGVQHINHQVEDIDDVMRSYYF
                     LREKGVKIVFGPGRHPSSSAVFLYFEGPDGMVYEYSVGVKHIRPEDEASYRPRQFPFS
                     PESFCMWGSRPDIPEFRKDEKPKGGKLKTAA"
     gene            complement(2979..4505)
                     /locus_tag="VI319_03120"
     CDS             complement(2979..4505)
                     /locus_tag="VI319_03120"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY6862866.1"
                     /translation="MNTRDAALAKVRELHHYIGGKHVKGEGGRFGDVYNPTTGALAAK
                     VPLASRAEVEKAIADSKRAFAEWSETSPVARARVMFRFKELVEKHIDELAAIVSSEHG
                     KVLSDAKGSVQRGLEVVEFACGIPHLMKGEYSDNVATGIDLYSIRQPIGVCAGITPFN
                     FPAMVPMWMFPVAIACGNTFVLKPSEKDPSTGLRLAELMLEAGAPAGVLNVINGDKEA
                     VDTLLADPRVAAVSFVGSTPIAKYVFATGSVNGKRVQALGGAKNHMIVMPDADIEVAA
                     VALMGAAYGSAGERCMAVSVAVPVTERTANELIEAMTPRIKKLKVGPSDDPASEMGPL
                     VTREHRDKVKGYVDLGVKEGASLVVDGRDFKVAGQPNGFFLGGSLFDKVRTDMRIYKE
                     EIFGPVLSVVRAKNFEEALALPSDHEYGNGVAIFTRNGDVARNFTHKVNCGMVGINVP
                     IPVPLSFHSFGGWKSSIYGDMNQHGPEGVRFYTRLKTVTARWREGVKPGASYGMPIFK
                     "
     gene            4617..5846
                     /locus_tag="VI319_03125"
     CDS             4617..5846
                     /locus_tag="VI319_03125"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6862867.1"
                     /translation="MSDAAIPHDRYAAFRVRDFRLFAVVVLLSAVLQQTQAVALGWDM
                     YNRTGSAMALGLIGLSQFVPAMLFFLPAGEIADRFDRRKVMIASFVLWGAASTGLAVA
                     AIAGAGVGWLYFWAAVSTTGHVINRPARDALLPQLVPGPVFPNAVVWSSSLYQLASIS
                     GPALAGSMIAVYGGASIVYGTNAALIVLALVLATRIPARAALPARRATTWGDLFAGLA
                     HVWRTPVVLGVMSLDLLAVIFASATALLPLYAKDILHVGPRELGWLSAAPAMGALAMA
                     FFQGAREPRHVGRAFFVSLAGYGLATAVFGMSTSFWLSLVALVCVGAMDNVSVVIRQT
                     VVQLYTPDEVRGRVSAVNRVFIQSSNQLGAFESGALAMLTTPVVAVVAGGIATVMIAA
                     AGLRLFPQLRRLGSLPR"
     gene            complement(5853..6992)
                     /locus_tag="VI319_03130"
     CDS             complement(5853..6992)
                     /locus_tag="VI319_03130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6862868.1"
                     /translation="MREAIFLLALASTNSGIALRVIEPMLPQLAADFGASVSETAIVI
                     IAYAIAQLIHGPLGDRFGKLRVVTLTLFCAGAGCYGCALAQGLASLSAMRFVTGAFAS
                     TPVILGMAYIGDRVAVAERQPVIARFVMGTISGQALGPFIGGAVTDLVGWRGTFALIG
                     TIFMVVATVLLVRTRSQWAGDLGKKFEGNPFATHLRLAHEPRVRRVLAVGAIETFFFF
                     GAFSFLGAYFKLKFGMSLTLAGAILAGFGVGGVLYGLMVRRLLMELGQRGLVLWGAIW
                     CCVFFAATALTPVWPVLFVCTIGLGYSFYMLHNTLQVKATEMSSTARGTAVAMYSSAW
                     AIGQAVGAAGMGLSIGWLDYAPSIVAFGAGYLLLGVWLRRNLEKL"
     gene            complement(7071..8045)
                     /locus_tag="VI319_03135"
     CDS             complement(7071..8045)
                     /locus_tag="VI319_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862869.1"
                     /translation="MIHATKTIQAAALALGLALAAPSFAQKYPDHPVRIVIPLSPGGT
                     TDVPGRIIAQKLSETMGQQFFVENRAGAGGTIGSDYVAKSKPDGYTLLLSATPFVIAP
                     HVYRKLPYDALKDFDPVMKIAEGPYVLVVHPSLGVSSVKELIALAKKQPGKIDFASSG
                     NGGAQHLVTELFMYMAGIKMNHVPYKGSGPAQQDLMSGIVKVSFVGTPIAIPQTKSGR
                     LKALGVSTPKRWPDLPNVPTIAEAGVPGFEAVVWIGMLAPAGTPKEIVTQLSGEIKKL
                     LGDSEVKKLLDPTGMDPAPDTPQQFARFLREDYDKWGKVVKESGATVQ"
     gene            complement(8088..9011)
                     /locus_tag="VI319_03140"
     CDS             complement(8088..9011)
                     /locus_tag="VI319_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6862870.1"
                     /translation="MNSAVARPAIEGREHWTKKGDVRLFLWQKANAAGTAKQGTILFV
                     HGSSMASQPTFDLRVPGRPDSSVMEWFAKRGFDTWCMDHEGYGRSDKKRPINCDIPNG
                     ADDLKAGTDYILKTTGAKKLLVYGISSGALRAALFAQRHPERVARLALDAFVWTGEGS
                     ATLAERKKKLAQFAATNRRPIDKAFVHSIFDRDHPGTADEATIEAFADQIVALDDSVP
                     TGTYVDMCSKLPVVDPSKITVPTIIMRGQWDGIASFDDLIRFFERLPNPDKTFSVMAG
                     ISHASFQQKNYLMVYHILHAFFAQPEPVYRG"
     gene            9085..>10013
                     /locus_tag="VI319_03145"
     CDS             9085..>10013
                     /locus_tag="VI319_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6862871.1"
                     /translation="MTANLKVVERKNYVNGEFLTSKKQFTKLNPVDGTVVAQVHEADR
                     AMVDSAVQGARKALKGPWGKMPLQQRVDLLRKVADGVEKRFDDFLQAEVADTGKPVSL
                     ASHLDIPRGAANFRVFADIVRSYGTESYFLDTPDGKGAVNYALRKPLGVIGVIAPWNL
                     PLLLLTWKLAPALAAGNCIVAKPSEETPSTATLLAEVMHDCGVPPGVYNVVHGFGPDS
                     AGEFITTHPDVNGITFTGETTTGSAIMKAVAPTVKPISFELGGKNAGIVFADCDFDAA
                     VAGMANAIFSNCGQVCLAPERVYIERPIFDRFV"
BASE COUNT         1504 a         3444 c         3387 g         1678 t
ORIGIN      
        1 ttggtcttcg ccttgcccgt catcgcgtga tcgaatttct tgtcgccgat cagctcggcg
       61 tacgacacgc tcttgccgcc cggcgccgcg acgacgccgt cggcgaccgt gaggcgctcg
      121 accggggcgt cgagcttcga cgacgccagc ccgagcagcg tctggcgcgc ctcggccgcg
      181 gcctggcgca gctgcggccc ggcgcgcatc atcgtctggc tgcccaccgt gggcccctgg
      241 ttggggcaca gcgccgtgtc gcccatgacc atgtgcacgc gctcgaacgg cacgtcgagc
      301 tcgtcggcga cgagctgggc gagcgcggtc tgcacgccgg tgccgagctc gaccttcccc
      361 gtgtagacgc tgaccttgcc gtcgccgccg acggacagcc acgcgtcgag ctgctgcagc
      421 ttcgggttcg gccgcgcctg cgccggggcc tgggccagga cgtcgagggg aatcgagaag
      481 gagacgacga gcgctccggc gctcctgagg aactcgcggc ggtcgatctt catgcggcct
      541 ccccggcggc gcgcttcacc gcgcgcagga tgcgcatgtg cgtgccgcag cgacacaggt
      601 tgccgtccat ggccttccgg atgtcggcgt cgctcggctt cgggttcttc gcgaggaagg
      661 ccgcggcggt catcaccatg ccgttgatgc agtagccgca ttgcgccgcc tgctcttcga
      721 cgaaggcctt ctgcacggga tgcggtttct cgggcgtgcc cagaccctcg atcgtgacga
      781 ccttgccctt gaccgtcgag accgggacgg agcaggagcg catcgggcgg ccgtcgatat
      841 ggacggtgca cgcgccgcac tgcgcgaggc cgcagccgaa cttcggcccg ttcaactcga
      901 gctcgttgcg cagcacgtac agcagcggcg tgtcgggcgc cgccttgacg gcgtgcggct
      961 tgccgttgac gttgatcgtc gtcatgcgtc tttctccttc agcgcgccat cgcggcgcga
     1021 tagaaactgt cgtcgtagta cctggcggga tcggcgagcg tcttctgcgg cttgccgtat
     1081 ttcgagcgca gctgcagcac ggtgcgcacg ccttccatgt cgatctgcgc cttgcgctgg
     1141 aacccgtccg tgggcgagag caggatacgg tacgaggtct cggcgccctg cggcccggcc
     1201 tgcggcaggt tcttcaggaa gatcgcgatc gcttcgtcct tgttgcgcgg atcgtagagc
     1261 cattccaccg cgtcgacata ggcgcggatg tagccgatca ccgcctcgcg atgggcgtcg
     1321 gcccagctct tgcgcgcgcc gccgaccagg ccctggtagc gcccgagcac gtcgatcgcg
     1381 ttcgcgagcc ggttgaagcc cttcgcctcg gcctgcacct cgaacggcga gagcagcagc
     1441 gtgcccgcgt gcttctgctc catgagctgc tggaagcgct gcagcacgcc gcccgcggag
     1501 acggtctggt agtcgcggcc gtggacgagg cccttgcgct ccaggatctc gagcagcacg
     1561 aacgcgtagc cggtcgtgag cgcgtcgacc gagagggttt ttccgcgcag gtcgtcgaag
     1621 ctgcgcacgc tcgggatcgt gacgagacgc aggaagccgt tgtccccgcc catgaacgcg
     1681 aacaggtccg ggccgatctt cgggacctcg ccctgccctt cgcggtacgc gatcaggttg
     1741 tcgaccgcgg tcatggcgat gtcgaacctg ccgtcgatca ggttggtcag ctggaacacc
     1801 gagctcggcg tcggcgtgac cttcacctcg atgccgtcgc gcgcgaagaa gcccttctcc
     1861 tgcgcgaccc agaccggcca gttgaaaccg ccggggaaga cgatgaggtt gaccggcgtg
     1921 agcgtcctgg gaggctcggg cgcgacgctc gcgcacgaaa cgagcgcaaa cgcgagcgcg
     1981 accacggccg cacggaaaat ccgttgcatc gtctcctcct tcgtgtcgcg gtcaggcagc
     2041 ggtcttcagc ttgccgccct tgggtttctc gtctttcctg aattccggta tatccggccg
     2101 cgagccccac atgcagaatg actcgggcga aaacggaaac tggcgcgggc ggtagctcgc
     2161 ctcgtcctcg ggacggatgt gcttcacgcc gaccgagtac tcgtagacca tgccgtcggg
     2221 cccttcgaag tagaggaaca ccgccgagga cgacggatgc cgcccgggcc cgaagacgat
     2281 cttcacgccc ttctcgcgca ggaagtagta cgagcgcatg acgtcgtcga tgtcctcgac
     2341 ctggtggttg atgtgctgca ccccgggatg cggcgacggg aacagcgcga gcgaatggtg
     2401 gatcgtgtcg atgcgcagca gcggcgcatc gccgatccag tcggacacgc gcgcgttgca
     2461 gatcttcgtc cagaacgcct cgtcacgcgc cgcgttcgag gtgcgcagcc cgatgtggct
     2521 gaagtgcgtg atgccggcgt cgcgcgccgg gtggtagcgc aggcccgagt ggtacgggcg
     2581 cgacacgatc tcgatcctgt tgccgctcgg gtcgctcgac gcgacgaagg cgcgcacgcg
     2641 gcgctgctcg cactcgtcct tcgtgccgta gtggaccggg cggcccgcct tctcgagcgc
     2701 cgccgcggcg gcgtcgagcg cctccgggtc gcgcaggtcg aatccgaccg cgtgatcgtc
     2761 cgccctgccc tcgaagtaca cgagcgtgtg gtcgcgcgtg tcgccgcgca cgccggcctt
     2821 gtccgagcgg aagtacgcgg ccctgccctc gcgcgccacc agctgcagcc cgacgatcgt
     2881 ggtcgcgtac tcgaccgccg actcgaggtc gggcgtgccg atccggaggt agcggatgtc
     2941 gcgaaggtca atcacaggcg tcgatcacgc cgcgcagatc atttgaatat gggcatgccg
     3001 tagctcgcgc cgggcttcac gccctcgcgc cagcgcgcgg tcacggtctt gaggcgcgtg
     3061 tagaagcgca cgccctcggg cccgtgctgg ttcatgtcgc cgtagatcga gctcttccag
     3121 ccgccgaagc tgtggaagga gagcggcacg gggatcggca cgttgatgcc gaccatgccg
     3181 cagttgacct tgtgcgtgaa gttgcgcgcg acgtcgccgt tgcgcgtgaa gatggcgacg
     3241 ccgttgccgt actcgtggtc cgagggcaag gccagcgcct cctcgaaatt cttcgcgcgc
     3301 acgaccgaga gcaccggccc gaagatctcc tccttgtaga tgcgcatgtc ggtgcgcacc
     3361 ttgtcgaaca gcgacccgcc gaggaagaag ccgttcggct gcccggcgac cttgaagtcg
     3421 cggccgtcga cgaccaggct cgcgccttcc ttcacgccga ggtcgacgta gcccttcacc
     3481 ttgtcgcggt gctcgcgggt gacgagcggc cccatctcgg acgcgggatc atcggacggg
     3541 ccgaccttga gcttcttgat acgcggcgtc atcgcctcga tcagctcgtt cgcggtgcgc
     3601 tcggtcacgg gcaccgcgac cgacaccgcc atgcagcgct cgcccgccga gccgtaggcc
     3661 gcgcccatca gcgcgacggc cgcgacctcg atgtcggcgt cgggcatgac gatcatgtgg
     3721 ttcttggcgc cgcccagcgc ctggacgcgc ttgccgttca ccgagccggt cgcgaacacg
     3781 tacttcgcga tcggcgtcga gccgacgaag ctcaccgcgg cgacgcgcgg gtcggcgagc
     3841 agcgtgtcga ccgcctcctt gtcgccgttg atcacgttca gcacgcccgc gggtgcgccc
     3901 gcctcgagca tcagctcggc gagacgcagg ccggtggagg ggtccttctc cgagggcttc
     3961 agcacgaagg tattgccgca cgcgatcgcg accgggaaca tccacatcgg caccatcgcc
     4021 gggaagttga acggcgtgat gcccgcgcac acgccgatcg gctggcggat cgagtacagg
     4081 tcgatgccgg tcgcgacgtt gtcggaatac tcgcccttca tcaggtgcgg gatgccgcag
     4141 gcgaactcga ccacttccag cccgcgctgc accgagccct tggcgtcgga gagcaccttg
     4201 ccgtgctcgg acgagacgat cgcggcgagc tcgtcgatgt gcttctcgac cagctccttg
     4261 aaccggaaca tcacgcgcgc ccgcgccacg ggcgaggtct cggaccactc ggcgaaggcg
     4321 cgcttcgagt cggcgatcgc cttctccacc tcggccctgg acgcgagcgg caccttcgcc
     4381 gcgagcgcgc cggtggtcgg gttgtagacg tcgccgaagc ggccgccctc tcctttgacg
     4441 tgcttcccgc cgatgtagtg gtggagctcg cggactttcg cgagcgcggc gtcacgagtg
     4501 ttcatgcgga cctcatcgag aaaaatggag agcggcgagc gggtacggcc gggatgcggg
     4561 gaattatagc cggttcccgg atacaatccg tccgggcgcc ggagcgcccg ttttttatgt
     4621 ccgacgccgc cattccgcac gaccgctacg ccgccttccg ggtgcgcgac ttccgcctgt
     4681 tcgcggtggt cgtgctgctg tcggccgtgc tccagcagac gcaggccgtg gccctcggct
     4741 gggacatgta caaccggacc gggtcggcga tggcgctggg gctgatcggg ctctcgcagt
     4801 tcgtgcccgc catgctgttc ttccttcccg cgggcgagat cgccgaccgc ttcgaccggc
     4861 gcaaggtcat gatcgcctcg ttcgtgctgt ggggcgcggc gtccaccgga ctcgcggtcg
     4921 ccgcgatcgc aggcgccggg gtcggatggc tgtacttctg ggcggccgtc agcaccacgg
     4981 gacacgtcat caaccggccg gcgcgcgacg cgctgctgcc gcagctcgtc cccggcccgg
     5041 tgttcccgaa cgcggtcgta tggagctcga gcctgtacca gctggcgtcg atctccgggc
     5101 cggcgctcgc cggctcgatg atcgcggtct acggcggcgc gagcatcgtg tacggcacga
     5161 acgccgcgct gatcgtcctc gcgctggtgc tcgccacgcg catccccgcg cgcgccgcgc
     5221 tcccggcgcg ccgcgcgacc acctggggcg acctgttcgc gggactcgca cacgtctggc
     5281 gcacgcccgt cgtcctcggc gtcatgtcgc tcgacctcct cgccgtcatc ttcgcgagcg
     5341 ccaccgcgct gctgccgctc tatgcgaagg acatcctgca cgtcggcccg cgcgagctcg
     5401 gctggctctc ggccgcgccc gcgatgggcg cgctggccat ggcgttcttc cagggcgcgc
     5461 gcgagccgcg ccacgtcggc cgcgcgttct tcgtctcgct cgccgggtac gggctcgcga
     5521 cggcggtgtt cggcatgtcg acgtcgttct ggctgtcgct cgtcgcgctc gtttgcgtgg
     5581 gcgcgatgga caacgtcagc gtcgtgatcc ggcagaccgt tgtgcagctc tacacgccgg
     5641 acgaagtgcg cgggcgggtg tccgcggtga accgcgtgtt catacagtcg tcgaaccagc
     5701 tgggggcgtt cgagtcgggg gcgctggcga tgctgacgac gccggtggtt gcggtggtgg
     5761 ccggagggat cgcgacggtc atgatcgccg ccgcgggact gcggcttttc ccgcagctga
     5821 gaagactcgg atcgcttccg cgatagaaca cgctatagct tctcgagatt cctcctcagc
     5881 cagaccccga gcagcaagta tcccgcgccg aacgcgacga tcgaaggcgc gtagtccagc
     5941 catccaatgc tgagccccat ccccgccgcc cccaccgcct ggccgatcgc ccacgccgac
     6001 gagtacatcg cgaccgccgt gccgcgcgcg gtggacgaca tctcggtcgc cttcacctgc
     6061 agcgtgttgt gcagcatgta gaatgaatac ccgaggccga tggtgcacac gaacagcacc
     6121 ggccacacgg gcgtgagcgc cgtcgccgcg aagaagacgc agcaccagat cgcgccccag
     6181 aggacgaggc cccgctgccc cagctccatc agcagccgcc gcaccatcag cccgtagagc
     6241 acgccgccga cgccgaagcc ggcgaggatc gcgcccgcga gcgtgaggct catcccgaac
     6301 ttgagcttga agtacgcgcc gaggaacgag aaggcgccga agaagaagaa cgtctcgatg
     6361 gcgccgaccg cgagcacgcg ccgcacgcgc ggctcgtgcg cgagcctgag gtgcgtcgcg
     6421 aacgggttgc cttcgaactt ctttccgagg tcgccggccc actggctgcg cgtgcgcacg
     6481 agaagcacgg tcgcgacgac catgaagatc gtgccgatca gcgcgaaggt gccgcgccag
     6541 ccgacgagat cggtcaccgc gccgccgatg aaaggcccga gcgcctggcc cgagatcgtg
     6601 cccatcacga agcgcgcgat caccggctgc cgctccgcga ccgcgacgcg gtcgccgatg
     6661 taggccatcc cgaggatcac cggcgtcgac gcaaaggcgc cggtgacgaa gcgcatcgcc
     6721 gagagcgatg cgaggccctg cgcgagcgcg cagccgtagc agcccgcgcc ggcgcagaac
     6781 agcgtcagcg tgacgacgcg aagcttgccg aagcggtccc cgagcggccc gtgaatgagc
     6841 tgcgcgatcg cgtacgcgat gatgacgatg gcggtctcgg agacgctcgc gccgaagtcc
     6901 gccgcgagct gcggcagcat cggctcgatg acgcgcagcg cgatcccgga attggtgctc
     6961 gcgagcgcga gcaggaagat ggcctcgcgc atgtcatcct gagcgaagcg aaggatctgc
     7021 tttaggcgtc aagaagcaga ttcttcgcgc tgcgcgctca gaatgacgac ctactgaacg
     7081 gtcgcgccgg attccttcac gaccttgccc cacttgtcgt agtcctccct gaggaacctg
     7141 gcgaactgct ggggcgtatc gggcgcggga tccattccgg tcgggtcgag cagcttcttc
     7201 acctcgctgt cgcccaggag cttcttgatc tcgccgctca gctgggtcac gatctccttc
     7261 ggcgtgccgg cgggcgcgag catcccgatc cacaccacgg cttcgaagcc cggcacgccg
     7321 gcctcggcga tcgtcggcac gttcggcagg tcgggccagc gcttgggtgt gctcacgccg
     7381 agcgccttca ggcggcccga cttcgtttgc gggatcgcga tcggcgtgcc gacgaagctc
     7441 accttgacga tgcccgacat caggtcctgc tgcgcggggc cgctgccctt gtagggcacg
     7501 tggttcatct tgatgccggc catgtacatg aacagctcgg tcaccaggtg ctgcgcgccg
     7561 ccgttgcccg agctggcgaa gtcgatcttg cccggctgct tcttcgcgag cgcgatcagc
     7621 tccttcaccg agctcacgcc aagcgacgga tgcaccacca gcacgtaagg cccttcggcg
     7681 atcttcatca cgggatcgaa atccttgagc gcgtcgtacg gcagcttccg gtacacgtgc
     7741 ggcgcgatca cgaagggagt cgcgctgagc agcagcgtgt agccgtcggg cttcgacttc
     7801 gcgacgtagt cgctgccgat cgtgccaccg gcgccggcgc ggttctcgac gaagaattgc
     7861 tgccccatcg tctccgagag cttctgcgcg atgatgcgcc ccgggacgtc ggtcgtcccg
     7921 cccggcgaga gcgggatgac gatgcgcacc gggtggtcgg gatacttctg cgcgaaagac
     7981 ggcgcggcga gcgccaggcc cagggcgagc gccgccgcct ggatcgtttt cgttgcgtgg
     8041 atcatgtgtg ctctctcctt cgtcctcgtt gcttcccgcg gcctctccta ccccctgtac
     8101 accggctccg gctgcgcgaa gaaggcgtga aggatgtggt agaccatcag gtagttcttc
     8161 tgctgaaagc tcgcgtgcga gatgccggcc atcaccgaga atgtcttgtc ggggttcggc
     8221 agccgctcga agaaccggat caggtcgtcg aagctcgcga tgccgtccca ctgcccgcgc
     8281 atgatgatgg tcggcaccgt gatcttcgag ggatcgacca ccggcagctt cgagcacatg
     8341 tcgacgtagg tgccggtcgg caccgagtcg tcgagcgcga cgatctgatc ggcgaaggcc
     8401 tcgatcgtgg cctcgtccgc cgtgcccggg tggtcgcggt cgaagatgct gtgcacgaac
     8461 gccttgtcga tcgggcggcg gttggtcgcc gcgaactgcg ccagcttctt cttgcgctcg
     8521 gcgagcgtcg cgctcccctc gcccgtccac acgaaggcgt cgagcgcgag ccgcgcgacg
     8581 cgctcgggat ggcgctgcgc gaacagcgcc gcgcgcagcg cgcccgagga gatgccgtag
     8641 acgaggagct tcttcgcgcc ggtggttttc aggatgtagt cggtgccggc cttcaggtcg
     8701 tcggcgccgt tcgggatgtc gcagttgatc gggcgcttct tgtccgagcg gccgtagccc
     8761 tcgtggtcca tgcaccaggt gtcgaagccg cgcttcgcga accactccat caccgacgag
     8821 tccgggcgcc cgggaacgcg caggtcgaag gtcggctgcg acgccatcga tgagccgtga
     8881 acgaacagga tcgtgccctg cttcgcggtc ccggccgcgt tcgccttctg ccagaggaag
     8941 aggcgcacgt cgcctttctt cgtccagtgc tcccggccct cgatcgccgg gcgtgccacc
     9001 gccgaattca tcgctgctcc cgcggaaaag ttcgaagtgt agcattagaa tgtggggcgt
     9061 ccgcctcgcg ttcccggagc ccccatgacc gccaacctca aagtcgtcga gcgcaagaac
     9121 tacgtcaacg gcgagttcct cacctcgaaa aaacagttca ccaagctgaa tcccgtcgac
     9181 ggcacggtcg tcgcgcaggt gcacgaggcc gaccgcgcga tggtcgatag cgcggtgcag
     9241 ggggcgcgca aggcgctgaa aggtccgtgg gggaagatgc cgctgcagca gcgcgtcgac
     9301 ctgctgcgca aggtcgcgga cggggtcgag aagcgcttcg acgatttcct ccaggccgag
     9361 gtcgccgaca ccggcaagcc cgtctcgctc gcgagccatc tcgacatccc gcgcggcgcg
     9421 gcgaacttcc gcgtcttcgc cgacatcgtg cgcagctacg gcaccgagtc gtattttctc
     9481 gacacgcccg acggcaaggg cgcggtgaac tacgcgctgc gcaagccgct cggcgtgatc
     9541 ggcgtcatcg cgccgtggaa cctgccgctg ctgctgctca cgtggaagct cgcgcccgcg
     9601 ctcgccgccg gcaactgcat cgtcgccaag ccctcggagg agacgccctc caccgcgacg
     9661 ctcctcgccg aggtcatgca cgactgcggc gtgccgcccg gcgtgtacaa cgtcgtgcac
     9721 ggcttcgggc cggactccgc cggcgagttc atcacgacgc atcccgatgt gaacggcatc
     9781 accttcaccg gcgagacgac gacgggctcg gcgatcatga aggcggtcgc gccgacggtg
     9841 aagccgatct cgttcgagct cggcggcaag aacgccggca ttgtgttcgc cgactgcgac
     9901 ttcgatgccg cggtcgcggg catggcgaac gcgatcttct cgaactgcgg ccaggtgtgc
     9961 ctcgcgcccg agcgcgtcta catcgagcgg ccgatcttcg accgcttcgt cga
//
LOCUS       DATDMW010000072        22069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1264416, whole genome shotgun sequence.
ACCESSION   DATDMW010000072 DATDMW010000000
VERSION     DATDMW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22069
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264416"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1145
                     /locus_tag="VI319_03150"
     CDS             123..1145
                     /locus_tag="VI319_03150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY6862872.1"
                     /translation="MDVGVFDHLDLGAQSPERYYRERLEIAAAYDRAGFRSYHVAEHH
                     LTPLGMAASPSVFLAAVAQRTQRLRFGPMVYVSPLYPRLRLLEEIGMLDQMSGGRLEL
                     AFGRGALAVELRFFGVDPAQADRANQETIDFVLEALRSGAVTVDEEGGRTRRLPLAIP
                     ALQMPHPPLWYGVHSPQSAERAARLGMNIMTLDWAAQMRACTDRFRAVWKEARTGEPL
                     PRIGLARFIHVDRKAADARAVAARAYKRWLASFNYLSSLMGVKHLRPRPERFEEIIED
                     GRAFAGTPEAAAEFVARQTDEAGANYFVGQFAFGDLTLDETLRSVSLFASEVMPMLKH
                     EPVISK"
     gene            1158..2114
                     /locus_tag="VI319_03155"
     CDS             1158..2114
                     /locus_tag="VI319_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862873.1"
                     /translation="MRPLAALALALATTLALGQTYPSRTVKVIVGTSPGGSPDVFARM
                     VAQKMTDSGWGTVVVENRIGANGNIAAEQVSKSPADGYTAYVCDSAIWAINPHLYKKV
                     PYRPLEDFDGVSTIATLPTFLTIHPSVPATSYAEFVAYARAHPGKLAYGSSGNGSIHH
                     ITTELFKSMTGIRMVHVPYKGMGQAAPALIAGDVQVAFSSYTAMAAFAKAGKVRILAS
                     AEGKRAPALPDVPTLDELGLAGFDMASLLGMLVPAGTPRAEVEKLNAGVVAAVADPAI
                     REKMIAFGVGVGTSTPEEFDALRRAEYEKYAKLVKLSGAKLD"
     gene            2245..2610
                     /locus_tag="VI319_03160"
     CDS             2245..2610
                     /locus_tag="VI319_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862874.1"
                     /translation="MKNSKRSYSRFAVAATVVVALAGSIAARSASVDADIANGFDPIT
                     MGLEIGNYSGGTVLAGETVPYGSGPVAAHTRPTDEEIANGLDPVTMGLEIGNYSGGTV
                     LAGETVPYESATRVAARGR"
     gene            2725..4002
                     /gene="soxC"
                     /locus_tag="VI319_03165"
     CDS             2725..4002
                     /gene="soxC"
                     /locus_tag="VI319_03165"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEY6862875.1"
                     /translation="MKKDQLDVPAAPRPSQGRRAFLAQGAAIATGAASAGLTANAGAQ
                     TLAGQPWERAYGAPFDGYGQPSRFEQPVMRHYLKPYGDLAPGTGAALAPIERLEGIIT
                     PSGLHSNRSHSGVPDIDPKLHELLIHGLVARPLTFSMDALLSYPMTTRIHFVECSGNS
                     NRALAPQPLQIPAGAIHGNMSCSEWTGIPLRTLLEEAGVKPEAKWLLAEGADSAHMSR
                     SVPLEKALDDAMIALYQNGERLRPEQGYPVRLLLPGWEGNMNVKWLRRLKATSEPTQT
                     KDETSKYTDLRKDGRAMQFTYEMGVKSVITKPSSTMTLPRHGLYEITGLAWTGAGRIR
                     RVEVSTDGGATWKDAALTGEERPKAFARFRLPWRWTGGEALLQSRATDEKGNVQPARK
                     DWIALWSPGNIYHNNSIVSWSVAADGSVKNVYA"
     gene            3989..4522
                     /locus_tag="VI319_03170"
     CDS             3989..4522
                     /locus_tag="VI319_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6862876.1"
                     /translation="MYTRDHVVACIGAVILAACATQTGPAGPDLGRAASAEEIAARDI
                     SIPPSGAGLPAGAGTARQGAAVYAAKCVSCHGDKGAGKPADPLAGGIGTLATAKPVRT
                     VGSYWPHATTLFDYVRRAMPLREPLSLTNDEVYAVSAYVLWLNGIVREDERMDAKTLP
                     LVKMPNRDGFVDEWSKR"
     gene            4519..5310
                     /locus_tag="VI319_03175"
     CDS             4519..5310
                     /locus_tag="VI319_03175"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY6862877.1"
                     /translation="MRAAALLLAALASTAHAADPAAGAAKAGAVCAACHGANGVSVSE
                     GIPNLAAQRAGYLEAQLKFFKDGTRKEPGNTSRAAIMNAIAARLSAEDIADLAAYFAA
                     QPGAAAAAKSELLPNLAKTGVAFPQDYKTAFTRYATIFFPDRNQLRYYYANASAVRAA
                     KAGRPLPDGAMLFVEVYSTRPDVDGWPLADRLTGYAAMARDKGWGGDIPELLRNENWN
                     YALFTPEKRHRPGVNQAECLACHKPQGDVSYTFTLERLADAAKGG"
     gene            5323..6390
                     /locus_tag="VI319_03180"
     CDS             5323..6390
                     /locus_tag="VI319_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY6862878.1"
                     /translation="MQFGTVPWGPLFRGALAALAFVLVLSTQFFFQLELYDYWPWTDI
                     LQGWADYFLDLALPGGCVFAAVAAAASLPVRSPLTRHAVLLCSVALGALAGEWLQLLR
                     APLPVDVSVAGVLAAKVARWAVFGVLAYAAWVYHRRAADAAGRAHESELQRVQLEHQA
                     AEAQLQALRAQIEPHFLFNTLANIQRLYQTEPATGRTMLLNFIAYLRAALPQMRRDET
                     TLRQEVQLAQAYLDVLKVRMGERLRFRLDVPRELGEMAFPPLALSTLTENAVKHGLNP
                     LPEGGSIDIAARVEDRRLKVEVADTGAGLTESAGSGSGLANLRARLAALYGDEAALEL
                     RANAPRGIRATIAVPLRNARG"
     gene            6383..7087
                     /locus_tag="VI319_03185"
     CDS             6383..7087
                     /locus_tag="VI319_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862879.1"
                     /translation="MDDRAPVTRLAACGAALGACIALLEFAYYYALVPGAEQPALLAS
                     LVLGWGGEGVVLGVTLGLIEVRAAPRPLGPWSLVLGVALAATVGVLAWQTFMHLVLRE
                     RFGLQLLRDYAGQPVDLASVAVYHMWLMLLFGGLAAAVYGLRQRHARMLAALRAAELA
                     RETSQRRLAEARLAGLRARIDPESLFETLGRLERMYEADPPGADRLLEEVIASLRAAV
                     ADVRASLALTFPKEVI"
     gene            7084..7488
                     /locus_tag="VI319_03190"
     CDS             7084..7488
                     /locus_tag="VI319_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862880.1"
                     /translation="MKKNENIRRKLLIAGLGGLAAQLGAADADAQDPAKIMPRAYRVA
                     FENDQLRVLDFVGRPGMGVCGEGMHSHPAHLTVVLSDWQGIATGADGAKVRRDKKLGD
                     VFWSEAETHKVENTGRTPARVLLIELKTPKKA"
     gene            7499..8257
                     /locus_tag="VI319_03195"
     CDS             7499..8257
                     /locus_tag="VI319_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY6862881.1"
                     /translation="MSERRPTAVLAEDEPVLRAQLIELLAAAWPELEVVAECADGFQA
                     MRALDERRPDVLFLDIQMPGLTGLEIARRASGSHHVVFVTAHDDHAVAAFEQGAVDYV
                     MKPLTAARIATACARVKERLSAAPPDLARVLDALAARAAPARSYLRWINASLGAEVKL
                     ITVDEICYFQSDTKYTRAVTSASESLIRKSIKELLEELDPETFWQIHRSTIVNVAAIA
                     GVSRDFAGRLTVKLKSRKETLPVSQPFAHRFRQM"
     gene            8293..8847
                     /locus_tag="VI319_03200"
     CDS             8293..8847
                     /locus_tag="VI319_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862882.1"
                     /translation="MPMFDLDRFIADCRDAAAADPSHKLVREVVARAVSGPQAVMRAL
                     GEPKRAMLQKLYQSPELTVLNVVWAPNWTLMPHDHRMWAVIGMYTGREDNIFWRRVPG
                     GSKGLIEARGARSLCVGDAEPLGPDVIHSVTNPIGRLTGAIHVYGGDFFTAERSEWDP
                     ETLLEGRFDSERAVRRFEEANRNL"
     gene            complement(8844..9689)
                     /locus_tag="VI319_03205"
     CDS             complement(8844..9689)
                     /locus_tag="VI319_03205"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY6862883.1"
                     /translation="MALRVAKLADALGAEITGVDLSKPLAPAVFAEIRRAWLEHLVLR
                     FRGQKLGDPQLIAFTKLFGELDPPGPNPYGKSFLPEHPEINVISNIVVGGAPIGGLGD
                     GEAIWHADMTYVERPPMGAILHAIEVPESGGDTYWANMYLAYESLPLALGLKIEGREA
                     IHDATYNSAGQMRKGYSEVTDPRKAPGALHPLISVHPETGRKCLFLGRRRNSYIVGMD
                     LEESEDLLDALWAHASRPELVFRQQWRAGDVVIWDNRCTLHRRDAFDPSARRLMHRTQ
                     IKAAA"
     gene            complement(9689..10357)
                     /locus_tag="VI319_03210"
     CDS             complement(9689..10357)
                     /locus_tag="VI319_03210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6862884.1"
                     /translation="MGKTIELTASDGHKLSAYRAEPKGKPRGGLIVVQEIFGVNSHIR
                     SVADGYAADGYLAIAPAFFDRVQRGFEIGYSPEEVERGRTFIPKMHWDTAMKDAGAAL
                     AEAKPAGKVGILGYCWGGTVAWMAAARLPGLACAVCYYGGGIPNNANEQPKVPVMFHW
                     GETDHAIPVDAAKKVAAQHPKAQSFIYKAGHGFNCDQRGSYDAESAKVARGRSLEFLR
                     KHIG"
     gene            10460..10936
                     /locus_tag="VI319_03215"
     CDS             10460..10936
                     /locus_tag="VI319_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6862885.1"
                     /translation="MPVTELNHFLIIASDLERTKDFYCDVLGFEVMPRPNFPFPGYWL
                     GINGKIQVHMAQAGVDNAELYYLGTPKNAARGSSGVIDHVAFLATEPEKMVKHLQKLG
                     VAARPRNFPESGLYQLFLKDPDGLTIELNFFGVEKAPDWGGEDYSKMPRSQEKARA"
     gene            10933..12138
                     /locus_tag="VI319_03220"
     CDS             10933..12138
                     /locus_tag="VI319_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6862886.1"
                     /translation="MNAPHRTPSFAGVAYDDALSRARALVPALRERAARAAAARAMPP
                     ETIADLHASGALRTLQPKRYGGMELDFVAYADFPYEIARGCASTGWNLANLQVHHWML
                     AQYDERAQEEIWGANPEALIASGIAFPQGRATKADGGFVISGRWNFSSCSNVAEWNML
                     AATVREGERAVGYRMCLLHKSQYQVIDDWKVLGMRSTGSMTVTAKDVFVPEHKALDML
                     LVRGGANFPGAKANPNPAYQVAANPLLGHGIGMCAVGNAQAALEHTTDLVKERSTNYT
                     GMRMRDFQGVQLRVGIAGAKIDAARLLLRNDCVEGQDYAKRGVIPDVQTKLAFKRNLA
                     FAVNLCTEAVDLLHAVAGANGIYESYPLERIFRDAHALAGHISFNIDAQASAWGLAAL
                     GGEVVNPTL"
     gene            complement(12135..13127)
                     /locus_tag="VI319_03225"
     CDS             complement(12135..13127)
                     /locus_tag="VI319_03225"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6862887.1"
                     /translation="MRKTPRTIDTHAHWYPREWLEMFARDGAGEGAKLERDGAKYVVR
                     TERIQNGFTEEFVDLERRIKGMNRTGVDVHAMSLTTPMVYFASPGLGLALAQCYNDCA
                     SAAHAKYPERLFGLAQAPMQAPELAVREVERAAKLPGIKGLYLATNINGTDLDDKKFW
                     DVYAACEALGWTVFLHPVDTVGRDRTGKYYLRNLCGNPYDTGLAAAHLIFGGVLDRFP
                     KLSFNLPHAGGTMPGLIGRWDHGTRVRPELAHMKKKPSAYLRRFTYDTIAHDDAIAMN
                     LVRQVGADRVVLGSDYCFDMGLDQPVKTVTRWKGLKPAERDLILGKTAARMLKI"
     gene            13213..14196
                     /locus_tag="VI319_03230"
     CDS             13213..14196
                     /locus_tag="VI319_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862888.1"
                     /translation="MKRLSIALGGFLALAAAAALAQSADSYPNHPVKIIVPYAPGGAT
                     DVIARIVGARLTDSLGQSFIVENRPGATGNLALELVAKSPADGYTLFVGNVSTNTINE
                     NTFANTLKIKPSRDLVGIAKLVELPHILAASTAFPANTVQELIAEAKKNPGKINYGSA
                     GIGSYPHLDMEKFAKAAGIQLTHVPYKDGAAKMVPSLLSNETQVAFINLGSTLAHVKS
                     GRMKALATAAPHRLAELPDVPTMTELGFAGIGTNAWQGMFAPAATPKPIVDKIYNAVA
                     AILTKPEMKESLAKQMMSVELSSSPQAFTELVRKETAGWGEFLRQANIKIE"
     gene            14199..15824
                     /locus_tag="VI319_03235"
     CDS             14199..15824
                     /locus_tag="VI319_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY6862889.1"
                     /translation="MHDLVIEKDVEVSMRDGARLRADVFRPKGAGRCPAIINLGSYQK
                     DKLWVPPPDLEEAANEFMNWETVNPLWWVPHGYACVRVDGRGSGKSPGRTDPWSPSEA
                     RDFYDAIEWTGRQSWCSGRVGTLGISYYAMTQWLVAGLNPPSLAAMIPWEGAADMYRD
                     FGYHGGIFSYGFAVGWWNNHMAHHLLGKPQSTATDAFSTPWLWEYMKNNLDGQWWEGR
                     RPRWDRITQPVYTVGNWSGMGLHLRGNTEGYLRAASKHKKLRIHAGTHYHPFYAEEAR
                     RDQLRFFEHWLKGERTGIMDEPPVKLLVRKGGHGNAEWRSENEWPLARTKWTKLYLAE
                     GRLSPDAPKQSASIAYSASGMTKAGVASASWTSTALAGSLPDMGASFLTEPMKEDTEV
                     TGPVVLVLWASSSTEDLDVFATIRNVDPDGKDVWELGQQSQPVPVAKGWLRASHRKKD
                     EALSTPWRPYHEHLQREWLKPGEVVQMEIEIWPTSMVFKKGHRIRLDVQPRDGAGSAP
                     YTHYAADYNTGTNTIFTGGQRASYLLLPIIPAK"
     gene            complement(15849..16217)
                     /locus_tag="VI319_03240"
     CDS             complement(15849..16217)
                     /locus_tag="VI319_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862890.1"
                     /translation="MPDQDIAITEQDGIIRAVYRGRARYEAVSTLLRDVVRVAAEKQA
                     SRILFDLRAADYGHYHVEAVQHAQESHALGLNAGFRIAFLGSPGEPMLRYIETVSINR
                     GYQVRAFTAESEAEGWLRQP"
     gene            complement(16307..17176)
                     /gene="sigJ"
                     /locus_tag="VI319_03245"
     CDS             complement(16307..17176)
                     /gene="sigJ"
                     /locus_tag="VI319_03245"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HEY6862891.1"
                     /translation="MTLDRAFTELRPAMFALAYRITGNRADAEETVQDAFVRLHDAAP
                     KDAVRSLKAYLTTITARLSLNRLRDQRARRETYVGEWLPEPLVTQDEPGVSAQDVSFA
                     LVAVLERLTPLERVVFVLRNAFDFSFKEIARVVGRDAVTCRKVFSRARTRVLEERPRF
                     AVDRERHRALLRSFAEAAQGGDKAALVSLLADDVVLHGDGGGKALATKKPVVGSAAVA
                     QLIIAVTRAVGADGLQETELNGAPALVVSKAGKPVVAIMIDSDGERIRSVFAVANPDK
                     LDALARPRARPSP"
     gene            17319..18239
                     /locus_tag="VI319_03250"
     CDS             17319..18239
                     /locus_tag="VI319_03250"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEY6862892.1"
                     /translation="MTTSPGTADPHPRRTIRVLDAEMSYVDTGGGDPIVFLHGNPTSS
                     YLWRSVIPHLGGLGRCLAPDLIGMGRSGKSPRRAYRFADHAAYLDAWFDTLGLSRNVI
                     LVVHDWGSALGFHRAFRFPRHVQAIAYMEAIVLPMRWDDFGEAAGIFRALRSEQGERM
                     VLDENFFVETVLPRNVLRALAPQEMAAYRAPYLDREARLPTLVWPRQIPVDGEPADVA
                     AIVARYGEWLSRSALPKLLVRSEPGAIMKAGGRPLEFCRGWPNQSEVTVPGGHFLPED
                     SPAEIGAALAAFVRALRVPAMRGDRAKATS"
     gene            18236..18781
                     /locus_tag="VI319_03255"
     CDS             18236..18781
                     /locus_tag="VI319_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY6862893.1"
                     /translation="MKPARYHPALVALHWFLAVFIVLALALGMFVLKAIPNSSPQKIE
                     ALRAHIGGGAVIAVLMIVRFAIRTLSARPAPATAGHPLLDRIARISHYAFYGLVAGMV
                     ATGLATALLAGLPDIVFGGSGAPLPESFTVFPTRVAHGWIAKALVALISLHVLAALYH
                     QFVRRDGLLGRMGFGRRWHAA"
     gene            18793..19578
                     /locus_tag="VI319_03260"
     CDS             18793..19578
                     /locus_tag="VI319_03260"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEY6862894.1"
                     /translation="MNAGLSLALFSLFLIVAVTAWTNVAHRPLPSGAERAIAFSGVAA
                     ASGALALGPGALGYVVAGIALAVSAFMSFLTFMSGLPSQRPAVAVGRAAPDFRGRDAD
                     GNEFRLGDRAGSRVVLKFFRGTWCPYCVADLRGWNDLAPELRALGLELVAVSHDTVEE
                     LARFKRRHGYDITFVADPDLAIIRRYNLQNRNFTPKRGPFRDMAIPAAILIDVDGKVL
                     WMEQATDFRVRSHPAKVLAAMRAALPAAARKPAHPGYVAAEAL"
     gene            19575..20024
                     /locus_tag="VI319_03265"
     CDS             19575..20024
                     /locus_tag="VI319_03265"
                     /inference="COORDINATES: protein motif:HMM:NF025607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HEY6862895.1"
                     /translation="MSKFWRIAPWLTRLPLALAAVLFSLIAFKYLSAPVANAAADAID
                     LGSVMAISRVRVGFGGFPLAFALIMLGCLLSSRRVLGGLIVLATTVAVVTAARLIGIA
                     VDGPAEEAVKLLVNEIVLLTLSIAALVLEQARLRRLDRLPIHPRRTE"
     gene            20021..20299
                     /locus_tag="VI319_03270"
     CDS             20021..20299
                     /locus_tag="VI319_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862896.1"
                     /translation="MKKSAIFLIAASVTLAATSSAAQDSMQKNGMPHDSMSKDPAMDH
                     TAKPAAANKGRKKSAMPRDTMGKDSMDKDSMGKDSMGKDPMEKSPAAM"
     gene            complement(20357..21397)
                     /locus_tag="VI319_03275"
     CDS             complement(20357..21397)
                     /locus_tag="VI319_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY6862897.1"
                     /translation="MAFVRVVLVSIASLMLAGPVFAQAKWAKAAPFPDPSEELLGASA
                     NGKMYVFCGLFETPRWTPKGLVYEYDPATDKWTKKAPMALPSHHVAFAELNGKIYAFG
                     GFVLPPKGEPPAWVPIDNAWEYDPAKDVWRALAPMPTRRGSPVAVAQSGKIYVIGGAV
                     PGPGQSAVHPARPHTSVGTVEEYDPATNTWRPRASMPTPRNHAVGGAIGGKIYVASGR
                     VGAAFISGGSSNIGVIEAYDPVKDSWSQGLARMAQARSAGAGAAYNGKLYITGGEYQD
                     PGMMGTYRSVEAYDPATNTWTFMPPMPVSRHGLAGAVVGSKLHMVSGDVQSAGSGVRV
                     DTEEHDVLELGK"
     gene            complement(21427..>22069)
                     /locus_tag="VI319_03280"
     CDS             complement(21427..>22069)
                     /locus_tag="VI319_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6862898.1"
                     /translation="CARKVAFYDRKVREGKWELPPGKPIFRLNGGTIGLVGFGNIARA
                     VALRAAAFGMKVLYADPFVKEGQFKEPGKKVELEQLLRESDFVSLHPPLLPQTRGMMN
                     DDAFAKMKKTAFVVNCARGPVIDTAALVRALDAKQIAGAALDTTDPEPLPDPHPLRGR
                     DNVIVNPHAAWYSEQAMVGLQRGAPGEVRRVLSGEWPINVVNKAVKGKSRAGL"
BASE COUNT         3290 a         7791 c         7679 g         3309 t
ORIGIN      
        1 ccgaagaaca agccgtcgat tctcatgttc atcatcggcg gcgacggccc gagcgccgag
       61 ttcacgagcg agtcgatggc tgcgctcgag cgcgcgggca tctggccacc gaaagcgtag
      121 ccgtggacgt cggcgtcttc gatcacctcg acctcggcgc gcagtcgccc gagcgctatt
      181 accgcgagcg actcgagatc gccgccgcgt acgaccgcgc gggcttccgc agctaccacg
      241 tcgccgagca ccacctgacg ccgctcggca tggcggcgtc gccctcggtg ttcctggccg
      301 cggtggcgca gcgcacgcag cggctgcgct tcgggccgat ggtgtacgtg tcgccgctct
      361 atccgcgcct gcggctgctc gaggagatcg gcatgctcga ccagatgagc ggcgggcggc
      421 tcgagctcgc gttcgggcgc ggcgcgctcg cggtggagct gcgcttcttc ggcgtcgatc
      481 cggcgcaggc cgaccgcgcc aaccaggaga cgatcgactt cgtgctcgaa gcgctgcgca
      541 gcggcgcggt caccgtcgac gaggagggcg ggcgcacgcg gcgcctgccg ctcgcgatcc
      601 ccgcgctgca gatgccgcac ccgccgctgt ggtacggcgt gcactcgccg cagtcggccg
      661 agcgcgcggc gcgcctcggc atgaacatca tgacgctcga ctgggcggcg cagatgcgcg
      721 cgtgcaccga ccggtttcgc gcggtgtgga aggaggcgcg caccggcgag ccgctgccgc
      781 gcatcggcct cgcgcgcttc attcacgtgg accgcaaggc ggcggacgcg cgcgcggtcg
      841 ccgcgcgcgc gtacaagcgc tggctcgcga gcttcaacta tctctcgtcg ctgatgggcg
      901 tgaagcacct gcgcccgcgg cccgagcgct tcgaggagat catcgaggac gggcgcgcct
      961 tcgccggaac gcccgaggcg gcggccgagt tcgtcgcgcg ccagaccgac gaagccggcg
     1021 cgaactactt cgtgggccag ttcgctttcg gcgatctcac gctcgacgag acgctgcgct
     1081 cggtgagcct cttcgcctcg gaagtcatgc cgatgctgaa gcacgaaccc gtcatctcga
     1141 agtaagtagg agccgccatg cgtccgctcg ccgccctcgc gctcgctctc gccacgacgc
     1201 tcgctctcgg acagacgtac ccttcccgca ccgtgaaggt catcgtcggc acgtcgcccg
     1261 gcggctcgcc cgacgtgttc gcgcgcatgg tggcgcagaa gatgaccgat tcgggctggg
     1321 gcacggtggt cgtcgagaac cgcatcggcg cgaacggcaa catcgccgcc gagcaggtgt
     1381 cgaagtcgcc cgccgacggc tacaccgcgt acgtctgcga cagcgcgatc tgggcgatca
     1441 acccgcatct gtacaagaag gtgccgtacc ggccgctcga ggacttcgac ggcgtctcga
     1501 ccatcgccac gctgccgacc ttcctcacga tccatccctc cgtgccggcg acgagctacg
     1561 ccgagttcgt tgcgtacgcc agggcgcacc ccgggaagct cgcgtacggc tcgtcgggca
     1621 acggctcgat ccaccacatc acgaccgagc tgttcaagtc gatgaccggc atccggatgg
     1681 tgcacgtgcc gtacaagggc atggggcagg cggcgccggc gctgatcgcg ggcgacgtgc
     1741 aggtcgcgtt ctcgagctac acggcgatgg cggcgttcgc gaaggcgggc aaggtgcgca
     1801 tcctcgcgag cgccgagggc aagcgcgcgc cggcgctgcc cgacgtgccg acgctcgacg
     1861 agctcggcct cgcgggcttc gacatggcgt ccctgctcgg catgctcgtg cccgcgggaa
     1921 cgccgcgcgc ggaagtggag aagctcaacg ccggcgtcgt cgccgcggtc gcggatcccg
     1981 cgatccgcga gaagatgatc gccttcggcg tcggcgtcgg cacgagcacg ccggaggagt
     2041 tcgacgcgct gcggcgcgcc gagtacgaga agtacgcgaa gctggtgaag ctttccggcg
     2101 ccaagctcga ctgaagagca cggcactttc cctcccctgt ttccggcgct tcgtcgcgct
     2161 tttttcgcct tcgtcgcatg acggtcacgg cgccctggcg ccgccgaata atggctcccg
     2221 tattcactca cgaggagaac cgtcatgaaa aattccaagc gctcgtactc gcgtttcgcc
     2281 gttgccgcca ccgtcgtggt ggcgctcgcc ggctccatcg ccgcccgctc ggcctcggtc
     2341 gacgcggaca tcgccaacgg cttcgatccg atcacgatgg gcctcgagat cggcaactac
     2401 tcgggcggga ccgtgctcgc cggcgagacg gtcccctacg ggtccggccc ggtcgccgcg
     2461 cacacccgcc ccaccgacga ggagatcgcg aacgggctcg acccggtcac gatgggcctg
     2521 gagatcggca actactcggg cgggaccgtg ctggcgggcg agacggtgcc gtacgagtcc
     2581 gccacccgcg tcgccgcccg cggccggtag cccgatccgt cgccgcattc ggggcgttcg
     2641 tcgccgattg ctggcgttcg tcgcaagccc gttgccgccg gcgcgctccg gcggcaacgt
     2701 ctcgcgaaac acgggagaac ccacatgaaa aaagaccagc tcgacgtgcc cgccgcaccc
     2761 cggccctcgc agggccgccg ggcgttcctc gcccagggcg cggcgatcgc caccggcgcc
     2821 gcatccgcgg gcctgaccgc gaacgcgggc gcgcaaaccc tggccggcca gccgtgggag
     2881 cgcgcctacg gtgcgccctt cgacggctac ggacagccct cgcgcttcga gcagccggtg
     2941 atgcgccatt acctcaagcc ctacggcgat ctcgcgcccg ggacgggcgc ggcgctcgcg
     3001 ccgatcgagc ggctcgaggg catcatcacg ccgagcgggc tgcactcgaa ccgcagccac
     3061 agcggcgttc ccgacatcga cccgaagctg cacgagctgc tgatccacgg cctggttgcg
     3121 cgcccgctga cgttctcgat ggacgcgctg ctctcctacc cgatgaccac gcgcatccac
     3181 ttcgtcgagt gctccggcaa cagcaaccgg gcgctcgcgc cgcagccgct gcagattccc
     3241 gcgggcgcga tccacggcaa catgtcgtgc agcgagtgga ccggcatccc gctgcgcacg
     3301 ctgctcgagg aagccggcgt gaagccggaa gcgaaatggc tgctggccga aggcgccgac
     3361 tcggcgcaca tgagccgcag cgtcccgctc gagaaggcgc tcgacgacgc gatgatcgcg
     3421 ctctaccaga acggcgagcg cctgcgcccg gagcagggct acccggtgcg cctgctgctg
     3481 cccgggtggg agggcaacat gaacgtgaag tggctgcggc ggctcaaggc caccagcgag
     3541 cccacgcaaa cgaaggacga gacgtccaag tacaccgacc tgcgcaagga cggccgcgcg
     3601 atgcagttca cctacgagat gggcgtgaag tcggtgatca ccaagccctc gtcgaccatg
     3661 acgctgccgc ggcacggcct gtacgagatc accgggctcg cctggaccgg ggcggggcgc
     3721 atccgccgcg tcgaggtgtc gaccgacggc ggcgcgacct ggaaggacgc ggcgctcacc
     3781 ggcgaggagc ggccgaaggc cttcgcgcgc ttccgcctgc cctggcgctg gacgggcggc
     3841 gaggcgctgc tgcagagccg cgccaccgac gagaagggca acgtgcagcc ggcgcgcaag
     3901 gactggatcg cgctgtggtc gcccgggaac atctaccaca acaactcgat cgtcagctgg
     3961 tcggtcgccg ccgacgggag cgtgaaaaat gtatacgcgt gatcacgtcg tggcctgcat
     4021 cggggcggtg atcctggccg cgtgcgcgac gcagacagga ccggccggac cggatctcgg
     4081 gcgcgcggcc agcgccgagg agatcgccgc gcgcgacatc agcatcccgc cgagcggcgc
     4141 gggcctgccc gccggcgccg gcaccgcccg ccagggcgcc gcggtctacg cggcgaagtg
     4201 cgtctcgtgc cacggcgaca agggcgcggg caagcccgcc gacccgctcg cgggcggcat
     4261 cggcacgctc gccacggcca agcccgtgcg caccgtcggc agctactggc cgcacgcgac
     4321 gacgctcttc gactacgtgc gccgcgccat gccgctgcgc gagccgctct cgctcaccaa
     4381 cgacgaggtc tacgcggtca gcgcctacgt gctctggctg aacggcatcg tgcgcgagga
     4441 cgaacgcatg gacgcgaaga cgctgccgct cgtgaagatg ccgaaccgcg acggcttcgt
     4501 cgacgaatgg agcaagcggt gagagccgca gcgctgctgc tggccgcgct cgcgtcgacg
     4561 gctcacgcgg ccgatcccgc ggccggcgcg gccaaggcgg gcgccgtgtg cgcggcctgc
     4621 cacggcgcga acggcgtgag cgtgagcgag ggcattccca acctcgccgc gcagcgggcg
     4681 ggctacctcg aggcgcagct gaagttcttc aaggacggca cgcgcaaaga gcccggcaac
     4741 accagccgcg cggccatcat gaacgcgatc gccgcccggt tgagcgccga ggacatcgcc
     4801 gacctcgcgg cgtatttcgc cgcccagccg ggggcggccg cggccgcgaa atcggagctg
     4861 ctgccgaacc tggcgaagac cggtgtcgct ttcccgcaag actacaagac cgccttcacg
     4921 cgctacgcga cgatcttctt ccccgaccgg aaccagctgc gctactacta cgcgaacgcg
     4981 tcggccgttc gcgcggcgaa ggccgggcgg ccgctgcccg acggcgcgat gctcttcgtc
     5041 gaggtgtatt ccacccggcc ggacgtcgac ggctggccgc tggccgaccg cctgaccggc
     5101 tatgcggcga tggcgcggga caagggctgg ggcggcgaca tcccggagct gctgcggaac
     5161 gagaactgga actacgcgct gttcacgccc gagaagcggc atcgcccggg cgtgaaccag
     5221 gccgaatgcc tcgcgtgcca caagccgcaa ggcgacgtca gctacacctt cacgctcgag
     5281 cggctcgccg atgctgcgaa ggggggctag aattgcggca cgatgcagtt cggcaccgtt
     5341 ccctggggtc ccctgttccg cggggctctg gctgcgctcg cattcgtgct cgttctttcc
     5401 acccagttct tcttccagct cgagctctac gactactggc cctggaccga catcctgcag
     5461 ggatgggccg attacttcct ggatctcgcg cttccgggcg gatgcgtgtt cgcggcggtg
     5521 gctgccgccg cgtcgctgcc cgtgcgctcc cccctcacca ggcacgccgt gctgctgtgc
     5581 tcggtcgcgc tgggcgcgct cgccggggag tggctgcagc tgctgcgcgc cccgctgccc
     5641 gtcgacgtgt ccgttgccgg cgtgctggcc gcgaaggtgg cgcgctgggc ggtgttcggg
     5701 gtgctcgcct atgccgcctg ggtctatcac cgccgtgccg cggacgccgc cgggcgcgcg
     5761 cacgaaagcg agctgcagcg cgtccagctc gagcaccagg ccgccgaagc gcagctgcag
     5821 gcgctgcgcg cgcagatcga gccgcatttc ctgttcaaca cgctcgcgaa catccagcgc
     5881 ctctaccaga ccgagccggc gacgggacgc accatgctgc tgaacttcat cgcctacctg
     5941 cgagcggcgc tgccgcagat gcgccgcgac gagacgacgc tgcgccagga ggtccagctc
     6001 gcgcaggcgt acctggacgt gctgaaggtg cgcatgggcg agcgcctgcg cttccgcctc
     6061 gacgtgccgc gcgagctcgg ggagatggcg ttcccgccgc tcgcgctgtc gacgctgacc
     6121 gagaacgcgg tgaagcacgg cctgaacccg cttcccgagg gcggctcgat cgacatcgcg
     6181 gcgcgcgtcg aggaccggcg cctgaaggtc gaggtcgcgg acaccggcgc ggggctgacg
     6241 gagagcgccg gcagcggctc cgggctcgcg aacctgcgcg cccggctggc ggcgctctac
     6301 ggcgacgagg cggcgctcga gctgcgggct aacgcgccgc gcggcatccg ggcgaccatc
     6361 gccgtgccgc tgaggaacgc gcgtggatga ccgcgcgccc gtgacgcggc tggccgcctg
     6421 cggggcggcg ctcggcgcgt gcatcgcgct gctcgagttc gcctactact acgcgctcgt
     6481 cccgggcgcg gagcagcccg cgctgctcgc gtcgctggtt ctcggatggg gcggcgaagg
     6541 cgtggtgctc ggcgtgacgc tcggcctgat cgaggtccgc gcggcgccgc ggccgctcgg
     6601 cccgtggtcg ctcgtgctcg gcgtggcgct cgcggcgacg gtcggcgtgc tcgcgtggca
     6661 gacgttcatg catctcgtgc tgcgcgagcg cttcgggctg cagctgctgc gcgactatgc
     6721 cggccagccc gtggacctgg cgagcgtcgc ggtttatcac atgtggctga tgctcctctt
     6781 cggcggactg gcggcggccg tgtacggctt gcgccagcgc cacgcgcgca tgctcgcggc
     6841 gctgcgcgcg gccgagctcg cgcgcgagac ctcgcagcgc aggctggccg aggcgcggct
     6901 cgccgggctg cgcgcgcgca tcgacccgga atcgctgttc gagacgctcg gccggctcga
     6961 gcgcatgtac gaagcggatc cgccgggcgc ggaccgcctg ctggaggaag tcattgcctc
     7021 gctgcgcgcg gcggttgccg acgtgcgcgc gagcctcgcg ctaacctttc ccaaggaggt
     7081 gatatgaaga agaacgagaa catccgcagg aagctcctga tcgcagggct gggtggcctg
     7141 gcggcgcagc tcggcgccgc cgacgccgat gcacaggacc cggcgaagat catgccgcgg
     7201 gcctaccgcg tcgcgttcga gaacgaccag ctgcgcgtgc tcgacttcgt cggccggccc
     7261 gggatgggcg tgtgcggcga aggcatgcac tcgcatcccg cgcacctgac ggtcgtcctc
     7321 agcgactggc aaggcatcgc gaccggcgcc gacggggcca aggtccggcg cgacaagaag
     7381 ctgggcgacg tcttctggtc ggaggccgag acgcacaagg tcgagaacac cggcaggacg
     7441 ccggcccgcg tgctgctcat cgagctgaag acgccgaaga aggcgtagcc gcggcggcat
     7501 gagcgagcgc cggcccaccg ccgtcctcgc cgaggacgag ccggtgctgc gcgcgcagct
     7561 gatcgagctg ctcgccgcgg cctggcccga gctcgaggtc gtggccgagt gcgccgacgg
     7621 cttccaggcg atgcgcgcgc tcgacgagcg caggcccgac gtgctgttcc tcgacatcca
     7681 gatgccgggg ctcaccgggc tcgagatcgc gcggcgcgcg agcggcagcc accacgtggt
     7741 gttcgtgacc gcgcacgacg accacgcggt ggcggcgttc gagcagggcg cggtggacta
     7801 cgtgatgaag ccgctcaccg cggcgcggat cgcgaccgcg tgcgcgcgcg tcaaggagcg
     7861 gctctcggcc gcgccgcccg acctcgcgcg cgtgctggat gcgctcgccg cgcgcgcggc
     7921 gcccgcgcgc tcgtacctgc gctggatcaa cgcgtcgctc ggcgcggagg tgaagctgat
     7981 caccgtcgac gagatctgct atttccagtc cgacaccaag tacacgcgcg ccgtgacgtc
     8041 ggcgagcgag tcgctcatcc gcaagtcgat caaggagctg ctcgaggagc tcgaccccga
     8101 gacgttctgg cagatccacc gctcgaccat cgtgaacgtc gccgcgatcg ccggcgtctc
     8161 gcgcgacttc gcggggcgcc tgacggtgaa gctgaagagc cgcaaggaga cgctgccggt
     8221 cagccagccc ttcgcgcacc ggttccggca gatgtagcgg agcgcgttat catgggccga
     8281 gggtgaccga ccatgcccat gttcgacctc gaccgtttca tcgccgactg ccgcgacgcc
     8341 gcggcggccg acccgtcgca caagctcgtg cgcgaggtgg tcgcccgcgc cgtgtccggg
     8401 ccgcaggcgg tgatgcgcgc gctgggcgag ccgaagcgcg cgatgctgca gaagctctac
     8461 cagtcgcccg agttgaccgt gctcaacgtc gtgtgggcgc ccaactggac gctgatgccg
     8521 cacgaccacc gcatgtgggc cgtgatcggc atgtacaccg ggcgcgagga caatatcttc
     8581 tggcgccgcg tcccgggcgg atcgaaaggc ctgatcgagg cgcgcggcgc gcgctcgctg
     8641 tgcgtcggcg acgccgagcc gctcggtccc gacgtcatcc actcggtgac caatcccatc
     8701 ggacgcctca ccggcgcgat ccacgtctac ggcggcgact tcttcaccgc cgagcgcagc
     8761 gagtgggatc ccgagacgct gctggagggc cgcttcgatt ccgagcgcgc ggtgcgccgg
     8821 ttcgaggagg cgaaccgcaa tctctaggcc gcggccttga tctgcgtgcg gtgcatgagg
     8881 cgccgcgcgg aagggtcgaa ggcgtcgcgg cggtgcaggg tgcagcggtt gtcccagatg
     8941 acgacgtcgc ccgctctcca ttgctggcgg aagacgagct ccggcctgga cgcgtgcgcc
     9001 cagagcgcat cgagcaggtc ctcgctctcc tcgaggtcca tgccgacgat gtagctgttg
     9061 cggcgccggc cgaggaacag gcacttgcgg ccggtctcgg ggtggacgga gatgagcgga
     9121 tggagcgcgc cgggcgcttt gcgcggatcg gtcacttccg agtagccctt gcgcatctgt
     9181 ccggcgctgt tgtaggtcgc gtcgtggatc gcctcgcgac cctcgatctt caggccgagc
     9241 gcgagcggca gcgattcgta cgcgaggtac atgttggccc agtacgtgtc gccgcccgac
     9301 tcgggcacct cgatcgcgtg caggatcgcg cccatcggcg ggcgctcgac gtaggtcatg
     9361 tccgcgtgcc agatcgcctc gccgtcgccc aggcccccga tcggcgcgcc gccgacgacg
     9421 atgttcgaga tgacgttgat ctcgggatgc tcgggcagaa acgacttccc gtagggattc
     9481 ggccccggcg gatcgagctc gccgaagagc ttcgtgaacg cgatcagctg cgggtcgccg
     9541 agcttctgcc cgcggaagcg cagcaccagg tgctcgagcc acgcgcgccg gatctcggcg
     9601 aacaccgccg gcgcgagcgg cttcgagaga tcgacgccgg tgatctcggc gccgagcgcg
     9661 tcggcgagct tcgcgacccg cagcgccatc agccgatgtg cttcctcagg aactcgagcg
     9721 agcggccgcg cgcgaccttc gcgctctcgg cgtcgtacga gccgcgctgg tcgcagttga
     9781 agccgtggcc ggccttgtag atgaacgact gcgccttcgg gtgctgcgcc gcgaccttct
     9841 tcgccgcgtc gaccgggatc gcgtggtccg tctcgcccca gtggaacatc acggggacct
     9901 tcggctgctc gttcgcgttg ttcgggatcc cgccgccgta gtagcacacg gcgcacgcga
     9961 ggcccgggag acgcgccgcg gccatccacg cgacggtccc gccccagcag tagccgagga
    10021 tgccgacctt gcccgccggc ttcgcctcgg cgagcgcggc gcccgcgtcc ttcatcgcgg
    10081 tgtcccagtg catcttcggg atgaacgtgc ggccgcgctc gacttcctcg gggctgtagc
    10141 cgatctcgaa gccgcgctgc acgcggtcga agaacgcggg cgcgatcgcg agatagccgt
    10201 cggcggcgta gccgtcggcg accgagcgga tgtggctgtt cacgccgaag atctcctgca
    10261 ccacgatcag gccgccgcgc ggcttgccct tcggctctgc gcggtaagcc gagagcttgt
    10321 ggccgtccga ggcggtgagt tcgatggtct tgcccacggg ctttctcctc cgggatgggt
    10381 tgaagcggga agcgcgaagt gtacttcgat ttggcataat gctccggttc gcccagcgcg
    10441 ccggatttcg aggtccacca tgcccgtcac cgagctgaac cacttcctga tcatcgcctc
    10501 cgacctggag cgcaccaagg acttctattg cgacgtgctc ggcttcgagg tcatgccgcg
    10561 cccgaacttc ccgtttcccg gctactggct cgggatcaac ggcaagatcc aggtgcacat
    10621 ggcgcaggcc ggcgtggaca acgccgagct ctattacctc ggcacgccga agaacgccgc
    10681 gcgcggcagc tcgggcgtga tcgaccacgt cgccttcctc gcgaccgagc ccgagaagat
    10741 ggtgaagcac ctgcaaaagc tgggcgtcgc ggcgcgtccg cgcaacttcc ccgagtcggg
    10801 gctctatcag ctctttctca aggatccgga cggcctcacg atcgagctca acttcttcgg
    10861 cgtcgagaag gcgcccgact ggggcggcga ggattattcg aagatgccgc gatcgcagga
    10921 gaaggcccgc gcatgaacgc gccgcaccgg acgccgagct tcgccggcgt cgcgtacgac
    10981 gacgcgttgt cacgcgcccg cgcgctggtc cccgcgctca gggagcgcgc cgcgcgcgcc
    11041 gcggccgcgc gcgcgatgcc gcccgagacg atcgccgacc tgcacgcgag cggcgcgctg
    11101 cgcacgctgc agccgaagcg ctacggcggc atggagctcg acttcgtcgc ctacgccgat
    11161 ttcccgtacg agatcgcacg cggctgcgcc tcgaccggat ggaacctcgc gaacctgcag
    11221 gtgcaccact ggatgctcgc gcagtacgac gagcgcgcgc aggaggagat ctggggcgcg
    11281 aacccggagg cgctgatcgc ctcgggcatc gccttcccgc aggggcgcgc gacgaaggcc
    11341 gacggcggct tcgtgatcag cggccgctgg aatttctcgt cgtgctcgaa cgtcgccgag
    11401 tggaacatgc tcgccgcgac cgtgcgcgag ggcgagcgcg cggtcggcta ccgcatgtgc
    11461 ctgctgcaca agtcgcagta ccaggtgatc gacgactgga aggtgctcgg catgcgctcg
    11521 acgggcagca tgaccgtgac cgcgaaggac gtgttcgtgc ccgagcacaa ggcgctcgac
    11581 atgctgctgg tgcgcggcgg cgcgaatttc ccgggcgcga aggccaaccc gaatcccgcg
    11641 taccaggtcg ccgccaaccc gctcctcggc cacggcatcg gcatgtgcgc ggtcggcaac
    11701 gcgcaggccg cgctcgagca cacgaccgac ctcgtcaagg agcgcagcac caactacacc
    11761 ggcatgcgca tgcgcgactt ccagggcgtg cagctgcgcg tcggcatcgc aggcgcgaag
    11821 atcgacgcgg cgcgcctgct gctccggaac gactgcgtcg agggccagga ctacgcgaag
    11881 cgcggcgtga tccccgacgt gcagaccaag ctcgccttca agcgcaacct cgcgttcgcg
    11941 gtgaacctgt gcaccgaggc cgtggacctg ctgcacgccg tcgcgggcgc gaacggcatc
    12001 tacgagagct atccgctcga gcgcatcttc cgcgacgcgc acgcgctcgc cgggcacatc
    12061 agcttcaaca tcgacgcgca ggcgtcggcg tgggggctcg cggcgctcgg cggcgaggtc
    12121 gtcaacccca cgctttagat cttcagcatc ctcgcggcgg tcttcccgag gatcagatcg
    12181 cgctcggcgg gcttgaggcc cttccagcgc gtcaccgtct tcaccggctg gtcgagcccc
    12241 atgtcgaagc agtagtcgct gccgaggacg acgcggtcgg cgccgacctg gcgcacgagg
    12301 ttcatcgcga tcgcatcgtc gtgggcgatg gtgtcgtagg tgaagcggcg gagatacgcg
    12361 ctcggcttct tcttcatgtg cgcgagctcg ggacgcaccc tcgtgccgtg gtcccagcga
    12421 ccgatcagcc cgggcatcgt gccgcccgcg tgcggcaggt tgaacgagag cttcgggaag
    12481 cggtcgagca cgccgccgaa gatcaggtgc gccgcggcga gcccggtgtc gtagggattg
    12541 ccgcagaggt tgcgcaggta gtacttgccg gtgcggtcgc gcccgacggt atcgaccggg
    12601 tgcaggaaga cggtccagcc gagcgcctcg cacgccgcgt agacgtccca gaactttttg
    12661 tcatcgagat cggtgccgtt gatgttggtg gcgagataga ggcccttgat gccgggaagc
    12721 ttcgccgcgc gctcgacctc gcgcaccgcg agctcgggcg cctgcatcgg cgcctgcgcg
    12781 agtccgaaca ggcgctcggg gtatttcgcg tgcgccgcgg aggcgcagtc gttgtagcac
    12841 tgcgcgagcg cgaggccgag gcccggcgag gcgaagtaga ccatcggcgt ggtgagcgac
    12901 atcgcgtgga cgtcgacgcc ggtgcggttc atccccttga tgcggcgctc gaggtcgacg
    12961 aactcctcgg tgaagccgtt ctggatgcgc tcggtgcgga cgacgtactt cgcgccgtcg
    13021 cgctcgagct tcgcgccctc gcccgcgccg tcgcgcgcga acatctcgag ccattcgcgc
    13081 gggtaccagt gcgcgtgggt gtcgatggtg cgtggggtct tgcgcatcgg tcctcctgtt
    13141 tcgagaggcc cgaatgttag cggcaaacag catcttgggc tatgatcggc gcaaaccttg
    13201 gaggaggtga ctatgaagcg attgtcgatc gcgctcggcg gctttctggc gctcgccgcg
    13261 gccgctgcgc tggcgcagtc cgcggattcc tatcccaacc acccggtcaa gatcatcgtg
    13321 ccttacgcgc ccggcggcgc gaccgacgtc atcgcgcgca tcgtcggcgc gcgcctgacc
    13381 gactcgctcg gccagagctt catcgtcgag aaccgacccg gcgcgacggg caacctcgcg
    13441 ctcgagctcg tcgccaagtc gcccgccgac ggctacacgc tgttcgtcgg caacgtctcg
    13501 accaacacca tcaacgagaa caccttcgcg aacacgctga agatcaagcc gtcgcgcgac
    13561 ctggtcggca tcgcgaagct ggtcgagctg ccgcacatcc tcgcggcgag cacggcgttc
    13621 cccgccaaca cggtgcagga gctcatcgcc gaggcgaaga agaacccggg caagatcaac
    13681 tacggctcgg ccggcatcgg cagctacccg cacctcgaca tggagaagtt cgccaaggcc
    13741 gcgggcatcc agctgacgca cgtgccgtac aaggacggcg cggcgaagat ggtgccgtcg
    13801 ctgctctcga acgagacgca ggtcgcgttc atcaacctcg gctcgacgct cgcgcacgtg
    13861 aagtccgggc gcatgaaggc gctcgcgacc gcggccccgc accggctcgc cgagctgccc
    13921 gacgtgccga cgatgaccga gctcggcttc gccgggatcg gtaccaacgc gtggcagggg
    13981 atgttcgcgc cggccgcgac gccgaagccg atcgtggaca agatctacaa cgcggtcgcc
    14041 gccatcctca ccaagcccga gatgaaggag agcctggcga agcagatgat gagcgtcgag
    14101 ctctcgagct cgccgcaggc gttcaccgag ctcgtgcgca aggagacggc cggctggggc
    14161 gagttcctgc gccaggcgaa catcaagatc gagtagtagt gcacgacctg gtcatagaaa
    14221 aagacgtcga ggtatcgatg cgcgacggcg cgcgcctgcg cgccgacgtg ttccgcccga
    14281 agggcgcggg gcgctgcccc gcgatcatca acctcggctc ctaccagaag gacaagctct
    14341 gggtgccgcc gcccgatctc gaggaggcgg cgaacgaatt catgaactgg gagaccgtga
    14401 acccgctgtg gtgggttccg cacggctacg cctgcgtgcg cgtcgacggc cgcggctcgg
    14461 gcaagtcgcc cgggcgcacc gatccgtggt cgccctcgga ggcgcgcgat ttctacgacg
    14521 cgatcgagtg gacgggacgg caatcgtggt gcagcggtcg cgtcggcacg ctcggcatct
    14581 cctactacgc gatgacccag tggctggtcg cgggcctcaa cccgccgtcg ctcgccgcga
    14641 tgatcccgtg ggaaggcgcg gccgacatgt accgcgactt cggctaccac ggcggcatct
    14701 tctcgtacgg gttcgcggtc ggctggtgga acaaccatat ggcgcatcac ctgctcggca
    14761 agccgcagag caccgccacc gacgccttct ccacgccgtg gctgtgggag tacatgaaga
    14821 acaatctcga cggccagtgg tgggaaggac gccgcccgcg ctgggaccgg atcacgcagc
    14881 cggtgtatac ggtgggcaac tggagcggca tgggcctgca cctgcgcggc aacacggaag
    14941 gctatctgcg cgccgcgtcg aagcacaaga agctcaggat ccacgccggc acgcattacc
    15001 acccgttcta cgcggaagaa gcgcggcgcg accagctgcg cttcttcgag cactggctga
    15061 agggcgagcg caccggcatc atggacgagc cgcccgtgaa gctcctcgtc cgcaagggcg
    15121 gccacggcaa cgccgagtgg cgcagcgaga acgagtggcc gctcgcgcgc acgaagtgga
    15181 cgaagctcta tctcgcggaa ggcaggctct cgcccgacgc gccgaagcaa tcggcgtcga
    15241 tcgcgtactc cgcgagcggc atgaccaagg ccggcgtcgc ctccgcgtcg tggacctcga
    15301 ccgcgctcgc gggcagcctc cccgacatgg gcgcgtcgtt cctgaccgag ccgatgaaag
    15361 aggacaccga ggtcacgggc cccgtcgtgc tcgtcctgtg ggcgtcgagc agcaccgagg
    15421 atctggacgt cttcgccacg atccgcaacg tcgaccccga cggcaaggac gtgtgggagc
    15481 tcggccagca gagccagccc gtgcccgtcg cgaagggctg gctgcgcgcg tcgcaccgga
    15541 agaaggacga ggcgctctcg acgccgtggc ggccttacca cgagcacctg cagcgcgaat
    15601 ggctgaagcc cggcgaggtg gtgcagatgg agatcgagat ctggccgacc tcgatggtgt
    15661 tcaaaaaggg ccaccgcatc cgcctcgacg tgcagccgcg cgacggcgcg ggcagcgcgc
    15721 cgtatacgca ctacgcggcg gactacaaca ccgggaccaa cacgatcttc acgggcgggc
    15781 agcgcgcatc gtatctgctg ctgccgatca tcccggcgaa gtaacccgta cgccgcgggc
    15841 cccgatcgtc acggctgtcg gagccagccc tcggcctcgc tctcagcggt gaacgcgcgg
    15901 acctggtaac cgcggttgat gctgacggtc tcgatgtagc gcagcatcgg ctcgcccggg
    15961 gacccgagga acgcgatgcg gaacccggcg ttgagaccca gcgcgtgact ctcctgcgcg
    16021 tgctgcacgg cctcgacgtg gtagtggccg taatccgccg cgcgcaggtc gaacaggatc
    16081 cggctcgcct gcttctcggc ggcgacgcgc acgacgtcgc ggagcaacgt gctcacggct
    16141 tcgtagcggg cgcgcccgcg gtacaccgcg cggatgatcc cgtcctgttc cgtgatcgcg
    16201 atgtcttgat ccggcatccg cacctcgagg agaacgcggt cgagcttact ccgggacccg
    16261 tgcagctaca agagccgcgg cggtgccgtc cgccggatgc gcgcgcttac ggggagggcc
    16321 gcgcgcgcgg gcgcgcgagc gcgtccagct tgtcggggtt cgctacggcg aacacggagc
    16381 ggatgcgctc gccgtcgctg tcgatcatga tggcgacgac cggcttgccg gccttgctca
    16441 cgacgagggc gggcgcgccg ttgagctcgg tctcctggag cccgtccgcg cccaccgcgc
    16501 gcgtcaccgc gatgatgagc tgcgcgaccg cggcgctgcc gacgacgggc ttcttcgtcg
    16561 cgagcgcctt gccgccgccg tcgccgtgca gcaccacgtc gtcggcgagc agcgagacca
    16621 gcgccgcctt gtcgccgccc tgggcggctt ccgcgaagct tcgcagcagg gcgcggtggc
    16681 gctcgcggtc caccgcgaag cgcggccgct cttcgagcac gcgggtgcgc gcccggctga
    16741 agaccttgcg gcaggtcacg gcatcgcgtc ccacgacgcg cgcgatctcc ttgaacgaaa
    16801 agtcgaacgc gttgcgcagc acgaagacga cccgctcgag cggcgtcagg cgctcgagca
    16861 ccgccaccag ggcgaacgag acgtcctgcg cgctcacgcc cggttcgtcc tgcgtgacga
    16921 gcggctcggg cagccactcg ccgacgtagg tctcgcggcg ggcgcgctga tcgcgcagcc
    16981 ggttgaggct caggcgcgcc gtgatggtcg tgagataggc cttcagcgac cgcaccgcgt
    17041 ccttcggggc ggcgtcgtgc aggcgcacga aggcgtcctg gaccgtttcc tcggcgtcgg
    17101 cgcggttgcc ggtgatgcgg taggccaggg cgaacatcgc cggccgcagc tcggtaaagg
    17161 cgcgatcgag cgtcatgtgt gccagaggct cctgtcctcc ttagacccgc gaggcgcaag
    17221 gaacgtgaca cccggccgaa atttattttc gcctgtcacg ccggcgtccc ggccggtgtc
    17281 ttgtccgcat gatcccacga ttcgaccagg agacacccat gacgacgagc ccgggcaccg
    17341 cggatccgca tccgcgccgc acgatccgcg ttctcgatgc cgagatgagc tatgtcgata
    17401 ccgggggcgg ggacccgatc gtcttcctgc acggcaaccc gacctcgtcg tatctgtggc
    17461 gcagcgtcat tccgcatctc ggcgggctcg ggcgctgcct cgccccggac ctcatcggca
    17521 tgggccgctc gggcaagtcg ccgcgccggg cctaccgctt cgccgaccac gcggcgtatc
    17581 tggacgcgtg gttcgacacg ctcgggctga gccggaacgt gatcctggtg gtgcacgact
    17641 ggggctcggc gctgggcttc caccgcgcgt tccgcttccc gcggcacgtg caggcgatcg
    17701 cgtacatgga ggcgatcgtg ctgccgatgc gctgggacga cttcggcgag gccgccggga
    17761 tcttccgggc gctgcgctcg gagcagggcg agcgcatggt cctcgacgag aacttcttcg
    17821 tcgagaccgt gctcccgcgc aacgtcctgc gcgcgctcgc gccgcaggaa atggccgcgt
    17881 accgcgcgcc ctacctcgat cgggaagcgc gcctgcccac gctcgtctgg ccgcggcaga
    17941 tcccggtcga cggcgagccg gcggacgtcg ccgcgatcgt cgcccgctac ggcgagtggc
    18001 tgtcgcgctc cgcgctgccg aagctgctgg tgcgcagcga gcccggcgcc atcatgaagg
    18061 cgggaggccg gccgctcgag ttctgccgcg gctggccgaa ccagagcgag gtgacggtcc
    18121 ccggcggaca tttcctcccg gaagactctc ccgcggaaat cggggccgcc ctcgcggcct
    18181 tcgtccgcgc gctgcgggtg ccggcgatgc gcggcgatcg cgcgaaggcg acctcgtgaa
    18241 gcccgcccgc tatcacccgg cgctcgtcgc cctgcactgg ttcctcgccg tgttcatcgt
    18301 cctcgccctc gcgctgggca tgttcgtgct caaggcgatc ccgaattcct cgccgcagaa
    18361 gatcgaggcc ctgcgcgccc acatcggcgg cggcgcggtg atcgccgtgc tcatgatcgt
    18421 gcgcttcgcc atccgcacgc tgagcgcgcg gcccgcgccg gcgacggcgg ggcacccgct
    18481 cctcgaccgc atcgcgagga tctcgcacta cgccttctac ggcctggtcg ccggcatggt
    18541 cgcgaccggc ctcgccacgg cgctgctcgc ggggctgccg gacatcgtct tcggtggctc
    18601 cggcgcgccg ctgcccgaat cgttcaccgt attccccacg cgcgtcgcgc acggctggat
    18661 cgcgaaggcg ctcgtcgcgc tgatctcgct gcacgtgctc gccgcgctct atcaccagtt
    18721 cgtgcgccgc gacggcctgc tcggacgcat gggattcggc cgtcgctggc acgccgcctg
    18781 aaggagaccg caatgaacgc cggactgtcg ctcgccctct tttccctttt cctgatcgtc
    18841 gccgtcaccg cctggacgaa cgtcgcccac cgcccgctgc cctccggggc ggagcgggcg
    18901 atcgccttct ccggcgtcgc cgccgcgtcg ggcgcccttg cgctcggccc gggcgcgctc
    18961 ggctacgtcg tcgcgggcat cgcgctcgcg gtgtcggcgt tcatgtcctt cctgaccttc
    19021 atgagcgggc tgccgtcgca gcgccccgcg gtcgcggtcg gacgcgcggc gcccgacttc
    19081 cgcggtcgcg acgcggacgg aaacgagttc cgcctcggcg accgggccgg gtcgcgcgtc
    19141 gtgctcaagt tcttccgcgg cacgtggtgc ccgtactgcg tcgccgacct gcgcggctgg
    19201 aacgacctcg ccccggagct gcgcgcgctc gggctcgagc tggtcgcggt cagccacgac
    19261 acggtcgagg agctcgcgcg cttcaagcgc cggcacggct acgacatcac gttcgtggcc
    19321 gatcctgatc tggctatcat ccgccgctac aacctgcaga accgcaactt cacgccgaag
    19381 cgcgggccgt tccgcgacat ggcgatcccg gcggcgatcc tgatcgacgt cgacggcaag
    19441 gtcctgtgga tggagcaggc gaccgacttc cgggtgcgct cgcatcccgc gaaggtcctc
    19501 gccgcgatgc gcgcggcgct tccggcggcg gcgcgcaagc cggcgcaccc cggatatgtc
    19561 gccgcggagg cgctgtgagc aagttctggc gcatcgcgcc gtggctgacg cggctgccgc
    19621 tcgctctcgc cgcggtgctc ttctccctca tcgcgttcaa gtacctgagc gcaccggtcg
    19681 cgaacgccgc ggccgacgcg atcgacctcg gctcggtcat ggcgatcagc cgggtgcgcg
    19741 tcgggttcgg cggatttccg ctggcgttcg ccctgatcat gctcggttgc ctgctgtcgt
    19801 cacggcgcgt gctcggcggg ctgatcgtgc tcgcgaccac cgttgccgtc gtcaccgccg
    19861 cgcggctgat cggcatcgcc gtcgacggcc cggccgagga ggccgtcaag ctgctggtca
    19921 acgagatcgt gctgctgacg ctctcgatag cagcccttgt cctcgagcag gcgcgactgc
    19981 gccggctcga ccgcttgccc atccacccaa ggagaacaga atgaagaagt ccgccatttt
    20041 cctgatcgcc gcttccgtca ccctcgccgc aacgagctcc gccgcgcagg attccatgca
    20101 gaagaacggg atgccgcacg actccatgag caaggatccc gcgatggatc acacggcgaa
    20161 accggccgcg gcgaacaaag gccgcaagaa gagcgccatg cctcgcgaca cgatgggcaa
    20221 ggactccatg gacaaggact ccatggggaa ggactccatg gggaaggatc cgatggaaaa
    20281 gagtccggcg gcgatgtaat gcggccgcgc tctctccgtt ccagcggcgt ccctccccgc
    20341 cgaggaggga tgtccgctat ttgccgagct cgagcacgtc gtgctcctcg gtgtcgacgc
    20401 gcacgcccga gcccgccgac tgcacgtcgc cgctcaccat gtgcagcttc gagccgacga
    20461 ccgcgcctgc gaggccgtgg cgcgagacgg gcatcggcgg catgaaggtc caggtgttgg
    20521 tcgcgggatc gtaggcctcg accgagcggt acgtgcccat catgccggga tcctggtact
    20581 cgccgccggt gatgtagagc ttgccgttgt aggcggcgcc cgctccggcg ctcctcgcct
    20641 gcgccatgcg cgcgaggccc tggctccagc tgtccttcac ggggtcgtag gcctcgatca
    20701 cgccgatgtt cgacgagccg cccgagatga acgccgcgcc gacgcgcccg ctcgcgacgt
    20761 agatcttgcc gccgatcgcg ccgccgacgg cgtggttgcg cggtgtcggc atcgacgcgc
    20821 gcgggcgcca ggtgttggtc gccgggtcgt actcctcgac ggtgccgacc gaggtgtgcg
    20881 ggcgcgccgg gtgcacggcg ctctgtcccg gtccggggac ggcgccgccg atcacgtaga
    20941 tcttgccgct ctgcgcgacg gcgacgggcg agccgcgccg ggtgggcatc ggcgcgagcg
    21001 cgcgccagac atccttcgcc ggatcgtatt cccaggcgtt gtcgatcggc acccacgcgg
    21061 gcggctcgcc cttgggcggg agcacgaagc cgccgaaggc gtatatcttg ccgttgagct
    21121 cggcgaaggc gacgtggtgc gagggcagcg ccatcggcgc cttcttcgtc cacttgtcgg
    21181 tcgcggggtc gtactcgtac acgagcccct tcggcgtcca gcgcggcgtc tcgaacagcc
    21241 cgcagaagac gtacatcttc ccgttcgccg acgcgccgag cagctcctcg gacggatcgg
    21301 ggaagggcgc ggccttggcc cacttcgcct gggcgaaaac cggccccgcg agcatcagag
    21361 aagcgatcga taccagaacg acgcgaacga acgccatggt tcttctcctc ctgaatcgtt
    21421 gatctactat aacccggccc ggctctttcc tttcacggcc ttgttgacca cgttgatcgg
    21481 ccactcgccc gacaggaccc ggcggacctc gcccggggcg ccgcgctgaa ggcccaccat
    21541 cgcctgctcg ctgtaccagg cggcgtgcgg gttgacgatg acgttgtcgc ggccgcgcag
    21601 cgggtgcgga tcgggcagcg gctccgggtc ggtggtgtcg agcgccgcac ccgcgatctg
    21661 cttcgcgtcg agcgcgcgca cgagcgccgc ggtgtcgatc accgggccgc gcgcgcagtt
    21721 cacgacgaag gcggtcttct tcatcttcgc gaaggcatcg tcgttcatca tgccgcgcgt
    21781 ctgcgggagg agcggcgggt gcagcgagac gaaatcggac tcgcgcagca gctgctcgag
    21841 ctccaccttc ttccccggct ccttgaactg gccttccttc acgaaaggat cggcgtagag
    21901 caccttcatg ccgaacgcgg cggcgcgcag cgcaaccgcg cgcgcgatgt tgccgaagcc
    21961 gaccaggccg atcgtgccgc cgttcaggcg gaagatcggc ttgcccggcg gcagctccca
    22021 ttttccttcg cgcaccttgc ggtcgtagaa cgcgaccttg cgcgcgcac
//
LOCUS       DATDMW010000073         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_890914, whole genome shotgun sequence.
ACCESSION   DATDMW010000073 DATDMW010000000
VERSION     DATDMW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890914"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..791
                     /gene="ugpQ"
                     /locus_tag="VI319_03285"
     CDS             42..791
                     /gene="ugpQ"
                     /locus_tag="VI319_03285"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY6862899.1"
                     /translation="MRSMKPWPHPRIIAHRGGGALAPENTLAALRRAKALGFAAVEFD
                     VMLSGDGVPLLMHDETLERTTSGRGAVAAAPYAAIAALDAGTKFGSAFAGEPVPGFAA
                     AAELCVELGLWANVEIKPAAGHARETGRVAAKLAAATWGRKSPAPLLSSFDPDALDAA
                     REAAPALERGYLVGRVPADWRGRLERHGCVSLNCDHVHLREADARAVKQSGYGLLCYT
                     VNDVDTARRLFDWGVDAIFTDRLDRIGPDFR"
     gene            complement(816..1322)
                     /locus_tag="VI319_03290"
     CDS             complement(816..1322)
                     /locus_tag="VI319_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY6862900.1"
                     /translation="MRLVKEGERAELDALIATIKAERGGRFLDLYAVLMNSPPIAEGW
                     LKLFTAIRQQSKLNGGMRELAILRVALLNGAAYEYRAHVPFALKEGVPQEKIDALEAW
                     RDSKLYSKEERAVLAYTDAMTRDIKVADDVFLSVRAAFDERETVELTATIAGYNLVSR
                     FLVAMQLH"
     gene            complement(1351..>2370)
                     /locus_tag="VI319_03295"
     CDS             complement(1351..>2370)
                     /locus_tag="VI319_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY6862901.1"
                     /translation="TLAGDDVQGLRRRIESGDLVKGEGRARTADVREAYLARIASDVR
                     LARPMKIVVDCGNGVAGATAGELYRRLGCEVTELYCEVDGSFPNHHPDPSQPANLRDL
                     IAKVRDIGAELGFAFDGDGDRLGVVTADGKNIFPDRQLMLFAQDVLSRKPGAEIIYDV
                     KSTRHLAPWIRKHGGVPSLWKTGHSLIKARLKERGALLAGEMSGHTFFVERWYGFDDA
                     QYAGARLLEILSKTRNATATLNALPDAVSTPELHLGLEEGEPHRLIERLQKEAQFPGA
                     DEIIRIDGLRVEYPRGFGLARASNTTPVIVLRFEADDGESLAKIQSEFRRVLLAAKPG
                     AKLPF"
BASE COUNT          325 a          856 c          818 g          371 t
ORIGIN      
        1 cagcgacgag ctgcggcgcc gcctctccca cggctgatac catgaggtcc atgaagccgt
       61 ggcctcatcc ccgcatcatc gcccatcgcg gcggcggcgc gctcgctccc gagaacacgc
      121 tcgcggcgct gcgccgcgcg aaggcgctcg gcttcgccgc ggtggagttc gacgtcatgc
      181 tttccggcga cggcgtgccg ctgctcatgc acgacgagac gctcgagcgc acgaccagcg
      241 gccggggcgc agtcgccgcg gcgccttacg cggcgatcgc cgcgctcgac gcgggcacga
      301 aattcggatc cgcgttcgcg ggggagccgg tgccgggctt cgccgccgcg gccgagctgt
      361 gcgtcgagct cggcttgtgg gccaacgtcg agatcaagcc ggccgcgggc catgcacgcg
      421 agaccggccg cgtcgcggcg aagctcgccg ccgcgacgtg gggcaggaaa tcgccggcgc
      481 cgctgctgtc gtcgttcgat cccgacgcgc tcgatgccgc gcgcgaggcg gcgcccgcgc
      541 tcgagcgcgg ctacctcgtc ggacgcgttc ccgcggattg gcgcggccgc ctcgagcgcc
      601 acggctgcgt gtcgctcaac tgcgaccacg tccacctgcg cgaggccgac gctcgcgcgg
      661 tcaagcagtc cggttacggg ctgctctgct acaccgtgaa cgacgtcgac accgcgcgca
      721 ggctcttcga ctggggcgtc gacgcgatct tcaccgaccg cctcgaccgg atcgggccgg
      781 atttccgctg acgctcggcc gacgcgaatg aggcctcagt gcagctgcat cgcgacgagg
      841 aagcgggaca ccaggttgta gcccgcgatg gtcgcggtga gctcgaccgt ctcgcgctcg
      901 tcgaaggcgg cgcgcaccga aaggaacacg tcgtcggcca ccttgatgtc gcgcgtcatg
      961 gcgtcggtat aggcgagcac ggcgcgctcc tccttcgagt acagcttgga gtcgcgccag
     1021 gcctcgagcg cgtcgatctt ctcctgcggc acgccttcct tcagcgcgaa gggcacatgc
     1081 gcgcggtact cgtacgccgc gccgttcagc agcgcgacgc gcaggatggc gagctcgcgc
     1141 atgccgccgt tcagcttcga ctgttgccgg atcgcggtga agagcttgag ccagccttcc
     1201 gcgatcgggg ggctgttcat cagcacggcg tacaggtcca ggaatcgccc gccgcgctcg
     1261 gccttgatgg tcgcgatgag cgcatccagc tccgcgcgct cgccttcctt caccagccgc
     1321 actctcgcca cttcgcctcc cttgggtgtt ctagaacggc agcttcgcgc ccggcttcgc
     1381 ggcgagcagc acccgcctga actcggactg gatcttcgcg agcgactcgc cgtcgtccgc
     1441 ctcgaagcgc agcacgatca cgggcgtcgt gttcgacgcg cgcgccagcc cgaagccgcg
     1501 cggatactcg acgcgcaggc cgtcgatacg gatgatctcg tccgcgccgg ggaactgcgc
     1561 ctccttctgc aggcgctcga tcaggcggtg cggctcgccc tcctcgaggc cgaggtgcag
     1621 ctcgggtgtc gacaccgcgt cgggcagcgc gttcagcgtc gccgtcgcgt tccgcgtctt
     1681 gctgaggatc tcgagcaggc gcgcacccgc gtactgcgcg tcgtcgaagc cgtaccagcg
     1741 ctcgacgaag aacgtgtgcc cgctcatctc gccggcgagg agcgcgccgc gctccttgag
     1801 ccgcgccttg atgagcgaat gcccggtctt ccacaggctc ggcaccccgc catgcttgcg
     1861 gatccatggc gcgaggtggc gcgtgctctt cacgtcgtag atgatctcgg cgccgggctt
     1921 gcgcgagagc acgtcctgcg cgaacagcat cagctgccgg tcggggaaga tgttcttgcc
     1981 gtcggcggtg accacgccga ggcggtcgcc gtcgccgtcg aacgcgaagc cgagctcggc
     2041 gccgatgtca cgcaccttgg caatgaggtc gcgcaggttg gccggctgcg acgggtcggg
     2101 atggtggttc gggaacgagc cgtcgacctc gcagtagagc tcggtgacct cgcagccgag
     2161 gcgccggtac agctcgcccg cggtcgcacc ggcgacgccg ttgccgcagt cgacgacgat
     2221 cttcatgggc cgcgcgagcc tgacgtcgga ggcgatgcgc gcgaggtagg cctcgcgcac
     2281 gtcggccgtg cgcgccctgc cctcgccctt gacgagatca cccgactcga ttcgccgccg
     2341 caggccctgc acgtcatcac ccgcgagcgt
//
LOCUS       DATDMW010000074         6652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1265182, whole genome shotgun sequence.
ACCESSION   DATDMW010000074 DATDMW010000000
VERSION     DATDMW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265182"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VI319_03300"
     CDS             complement(<1..676)
                     /locus_tag="VI319_03300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6862902.1"
                     /translation="MIIDFDSHLREGYFMDEVYRLEGPWERFRPVRLNDGRTHNTKFL
                     HSLDPISPQGHAAHRHPYIYDPKTNWRGGDIAARQVGGYDMARRREDIAREGIDKQLI
                     FPTQITVATSNLGGLGRECARLYNDWVAKLVRGHEDVLLPVAMAPAGCPEAMAEELRR
                     CVKQLGFRASHLVPYCGTRNLDDPAYFPYYAEAEALGVPLLCHPNSNGELVDRFDNFY
                     KTHVLGR"
     gene            complement(673..1095)
                     /locus_tag="VI319_03305"
     CDS             complement(673..1095)
                     /locus_tag="VI319_03305"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY6862903.1"
                     /translation="MNDKAATGFPIRIPFTEHLGVRLLEMTREHAVVSLEKRPELLNS
                     WGATHGGVIMTMLDLVMSWAVRGHYEAIAGVLTVDMSVGFMRAGFGERVVAEGRVLHG
                     GQSTAFCEGEARDEQGNLLAKAIGTFKLLADKEKKAGA"
     gene            complement(1106..1885)
                     /locus_tag="VI319_03310"
     CDS             complement(1106..1885)
                     /locus_tag="VI319_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6862904.1"
                     /translation="MMSVRKLFDLGGKTALITGGSRGLGLQIGEALGEMGARLIITAR
                     KADELEEARAHFAAQRIDALPLACDLSDPKAIEPMVRKALEARERIDVLVNNAGTTWG
                     APAEEHPLDAWQKQLNLNLTGTFLVTQAVGKLSMIPKRYGRIVNIASVAGLRGNPVGL
                     LKTLGYSTTKGGVVNFTRALAGEWGEYGITVNAIAPGFFPSKMTQGALKRIADQVIAA
                     TPLHRLGDDQDLKGLAVLLASDASRHITGQVIAVDGGATVV"
     gene            complement(1882..3060)
                     /locus_tag="VI319_03315"
     CDS             complement(1882..3060)
                     /locus_tag="VI319_03315"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY6862905.1"
                     /translation="MPDAVIVSTARTPIAKAWRGAFNMTHGAKLGGHAVHHAIERAGI
                     APAEVEDVIMGCAYPEGATGQNIARQVALRAGCPATTAGVTVNRFCSSGLQTIAMAAQ
                     RVIVDRVPVVVAGGVESISCVQNQANQHMLHDEWLVRHKPEIYWPMLQTAEVVSKRYD
                     IGRERQDEYGVQSQARAAAARAAGKTEAEMFPITVRMQKADKDSGKSWIEEVTISKDE
                     GIRADTSYEGVSKIKPAIEGGTIAAGNASQFSDGAAACVVMDAKLAEKRNMKPLGIFR
                     GFAVAACEPDEMGIGPVFAVPRLLSRTGVKMADIGLWELNEAFAVQVIYCRDRLGIPN
                     DILNVNGGAIALGHPYGMSGARLAGHALIEGRRRGVKHVVVTMCIGGGMGAAGLFEVV
                     "
     gene            3147..4796
                     /locus_tag="VI319_03320"
     CDS             3147..4796
                     /locus_tag="VI319_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HEY6862906.1"
                     /translation="MLRGLMMDMPLLVSSLIEHAGLYHGDTEMVSRLIEGPAHDGASA
                     VHRYTYADARKRAKQLAQALARLGVRQGDRVGTLAWNGFRHYELYFGVSGMGAVIHTI
                     NPRLFPEQLGYIVNHAEDAYVFFDLSFAPLVEKLAPQCRAVKGWVAMTDRAHMPSAQI
                     PNLLCYEELMAKEDGEYEWPELDERTAAGLCYSSGTTGNPKGVLYTHRSTVLHAYASS
                     LPDVFNCSSRSTVLAVVPMFHVNAWGIPYFGTLNGAKLVFPGAALDGKSLYELFEGEK
                     VTASAGVPTVWLGLLAYMKENKLRFGTLKTVVVGGSACPPAMIKAFQDEYGVACRHAW
                     GMTEMSPLGTFYSPKNRHDALSADARHQLECKQGRTIYGVQMKIVDGSGKELPRDGKA
                     FGDLLVRGPWVLREYFKAEDDPLRDGWFPTGDVATIDAEGYMQITDRSKDVIKSGGEW
                     ISSIDLENIAVAHPAIAEAAVIGVRHPKWDERPLVVAVKKAGASVTREELLAFYQGKV
                     AKWWMPDDVVFVEQLPHTATGKLLKTRLRQDFKDHRLPAGK"
     gene            4798..5565
                     /locus_tag="VI319_03325"
     CDS             4798..5565
                     /locus_tag="VI319_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY6862907.1"
                     /translation="MEPILTGRDGGVLRIELNRPEKKNAITIAMYQALGDALHSASGD
                     GAVRAVLLHGKPEVFCSGNDVADFLAAPSRSEDFAPFRFLRILGQFDKPLVAAVSGAA
                     IGIGTTMLLHCDLVYAAAGTRFQLPFASLGLVPEAASSLLLPRLAGWQKAAELLMLGE
                     PFGAETAREIGLVNEIVAPQAVLETARAKALALAAKPPAAMRATKALMKQAVLAEIEQ
                     TMTREVKTFGERLVSPEAKEALSAFLEKRKPDFSKFS"
     gene            5568..6389
                     /locus_tag="VI319_03330"
     CDS             5568..6389
                     /locus_tag="VI319_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6862908.1"
                     /translation="MTLKGKTLFITGASRGIGLAIALRAARDGANVVVAAKTTEAHPK
                     LPGTIYTAAEEIEKAGGKALPIALDIRDDAAVQQAAAKAVETFGGIDILVNNASAINL
                     TGTLATPMKRFDLMFGVNVRGTYCCSQACLPYLKKAANPHILTLSPPLNMKPKWFKDH
                     VAYTMAKYGMSMCVLGMAEEFRADGVAVNALWPRTVIQTAAIAMLGGLVKPENCRKPE
                     IVADAAYIILNRDSRRYTGHFAIDDEVLAAEGVTDLERYALVPGKARLLPDFFLD"
     gene            6444..>6652
                     /locus_tag="VI319_03335"
     CDS             6444..>6652
                     /locus_tag="VI319_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862909.1"
                     /translation="MKRMIAVVGGLCLLAGCTAMTTMTGTQSDVSIQVAERTYSSAPA
                     TDTFGATSFGNYEFKASRPGQEPVYG"
BASE COUNT         1025 a         2268 c         2313 g         1046 t
ORIGIN      
        1 gccgcccgag cacgtgcgtc ttgtagaaat tgtcgaagcg gtcgacgagc tcgccgttcg
       61 agttcggatg gcacagcagc ggcacgccga gcgcttcggc ctcggcgtaa taagggaagt
      121 acgcggggtc gtccaggttg cgcgtgccgc agtagggaac gaggtgcgag gcgcggaagc
      181 cgagctgctt cacgcagcgg cgcagctcct cggccatcgc ctcggggcag cccgcgggcg
      241 ccatcgcgac cggcagcagc acgtcctcgt gcccgcgcac cagcttcgcc acccagtcgt
      301 tgtacaggcg cgcgcactcg cggccgaggc cgccgaggtt gctcgtggcg acggtgatct
      361 gcgtcgggaa gatcagctgc ttgtcgatgc cttctcttgc gatgtcctcg cgccggcgcg
      421 ccatgtcgta gccgccgacc tggcgcgcgg cgatgtcgcc gccgcgccag ttggtcttcg
      481 ggtcgtagat gtacgggtgc cggtgcgcgg cgtgcccctg cgggctgatc gggtcgagcg
      541 agtgcaggaa cttggtgttg tgcgtgcggc cgtcgttcag gcgcaccggg cggaagcgct
      601 cccagggccc ctcgaggcgg tacacctcgt ccatgaagta gccttcgcgc aggtgcgagt
      661 cgaaatcgat gatcatgcgc ccgccttctt ctccttgtcg gccagcagct tgaaggtgcc
      721 gatcgccttg gcgaggaggt tgccctgctc gtcgcgcgcc tcgccctcgc agaaggcggt
      781 cgactggccg ccgtggagca cgcgcccctc ggcgacgacg cgctcgccga agcccgcgcg
      841 catgaagccg acgctcatgt cgacggtgag cacgccggcg atcgcctcgt agtgcccccg
      901 caccgcccag ctcatgacga ggtcgagcat ggtcatgatc acgccgccgt gcgtcgcccc
      961 ccagctgttc agcagctcgg ggcgcttctc gagcgagacg accgcgtgct cgcgcgtcat
     1021 ctcgagcagg cgcacgccga ggtgctcggt gaaagggatg cggatcggaa agccggtcgc
     1081 ggccttgtcg ttcatgggcc cgtttttata ccaccgtcgc gccgccgtcc accgcgatca
     1141 cctggcccgt gatgtggcgc gaggcgtccg aggcgagcag caccgcgagc cccttcaggt
     1201 cctggtcgtc gccgaggcgg tgcagcggcg tggcggcgat cacctggtcg gcgatgcgct
     1261 tcagcgcgcc ctgcgtcatc ttcgacggaa agaatcccgg cgcgatggcg ttcacggtga
     1321 tgccgtactc gccccattcg ccggcgagcg cgcgcgtgaa gttgacgacg ccgcccttgg
     1381 tcgtgctgta gccgagcgtc ttcagcagcc cgacgggatt gccgcgcagg ccggcgaccg
     1441 aggcgatgtt gacgatgcgg ccgtagcgct tcgggatcat ggagagcttc ccgaccgcct
     1501 gcgtgacgag gaacgtgccg gtgaggttca ggttcagctg cttctgccac gcgtcgagcg
     1561 gatgctcctc ggccggcgcg ccccaggtgg tgcccgcgtt gttcacgaga acgtcgatgc
     1621 gctcgcgcgc ctcgagcgcc ttcctcacca tcggctcgat cgccttcgga tccgagaggt
     1681 cgcacgcgag cggcagcgcg tcgatgcgct gcgcggcgaa gtgcgcgcgc gcctcctcga
     1741 gctcgtcggc cttgcgcgcg gtgatgatca gcctcgcgcc catctcgccc agcgcctcgc
     1801 cgatctgcag gcccagcccg cgcgagccgc cggtgatcag cgcggtcttg ccgccgagat
     1861 cgaatagctt cctgacgctc atcacaccac ctcgaacagc cccgccgcgc ccatgccgcc
     1921 gccgatgcac atggtgacga cgacgtgctt gacgccgcgg cggcggccct cgatcagcgc
     1981 gtggccggcg aggcgcgcgc ccgacatgcc gtatgggtgg ccgagcgcga tggcgccgcc
     2041 gttgacgttg aggatatcgt tcgggatgcc gagccggtcg cggcagtaga tgacctgcac
     2101 ggcgaacgcc tcgttcagct cccacaggcc gatgtcggcc atcttcacgc ccgtgcgcga
     2161 cagcagccgc ggcacggcga agacgggacc gatgcccatc tcgtcgggct cgcacgcggc
     2221 gaccgcgaag ccgcggaaga tgccgagcgg cttcatgttc cgcttctcgg cgagcttcgc
     2281 gtccatgacg acgcacgcgg cggcgccgtc ggagaactgg ctggcattgc cggcggcgat
     2341 ggtgccgccc tcgatcgcgg gcttgatttt ggagacgccc tcgtagctgg tgtcggcgcg
     2401 gatgccttcg tctttcgaga tcgtgacttc ctcgatccag ctcttgccgg agtccttgtc
     2461 ggccttctgc atgcgcaccg tgatggggaa catctccgcc tcggtcttgc cggccgcgcg
     2521 cgccgccgcg gcgcgcgcct ggctctgcac gccgtactcg tcctggcgct cgcggccgat
     2581 gtcgtagcgc ttcgacacga cctcggccgt ctgcagcatc ggccagtaga tctcgggctt
     2641 gtgtcgcacg agccactcgt cgtgcagcat gtgctggttc gcctggttct gcacgcacga
     2701 gatcgactcc acgccgcccg cgaccacgac cggcacgcgg tcgacgatga cgcgctgcgc
     2761 cgccatggcg atcgtctgca ggcccgagga gcagaagcgg ttcacggtca cgcccgccgt
     2821 ggtcgcgggg caccctgcgc gcagcgcgac ctggcgcgcg atgttctggc cggtcgcgcc
     2881 ctcgggatag gcgcagccca tgatcacgtc ctcgacctcg gccggcgcga tgccggcgcg
     2941 ctcgatcgcg tgatgcacgg cgtggccgcc gagcttcgcg ccgtgcgtca tgttgaaggc
     3001 gccgcgccag gccttggcga tcggcgtgcg ggcggtcgag acgatgacgg cgtcgggcat
     3061 tgaaaagcgc tgcgcagagg gtcgggacga ttctacaacc gtcgtcggtt gacagtgcgg
     3121 aggtcgcgcg gtaatttgca gcgaccatgc tgcgcggcct gatgatggac atgccgctgc
     3181 tcgtctcgtc cctgatcgag cacgcgggcc tgtatcacgg cgacaccgag atggtgtcgc
     3241 gccttatcga agggcccgcc cacgacggcg ctagcgccgt gcaccggtac acctacgccg
     3301 acgcgcgcaa gcgcgccaag cagctcgcgc aggcgctcgc gcgcctcggc gtgcgccagg
     3361 gcgaccgcgt cggcacgctg gcgtggaacg gcttccgcca ctacgagctc tatttcggcg
     3421 tctcgggaat gggcgccgtg atccacacca tcaacccgcg cctgttcccc gagcagctcg
     3481 gctacatcgt caatcacgcc gaggacgcct atgtcttctt cgacctgagc ttcgcgccgc
     3541 tcgtggagaa gctcgcgccg cagtgcaggg cggtgaaggg ctgggtggcg atgaccgacc
     3601 gcgcccacat gccctcggcg cagatcccga acctgctctg ctacgaggag ctgatggcga
     3661 aggaggacgg cgagtacgag tggccggagc tcgacgagcg caccgcggcg ggcctgtgct
     3721 acagctcggg cacgaccggc aacccgaagg gcgtgctcta cacgcatcgc agcaccgtgc
     3781 tgcacgcgta cgcctcgtcg ctcccggacg tgttcaactg ctcgtcgcgc tcgaccgtgc
     3841 tcgcggtggt gccgatgttc cacgtcaacg cctggggcat cccgtatttc ggcacgctga
     3901 acggcgcgaa gctcgtgttc cccggcgccg cgctcgacgg caagagcctg tacgagctgt
     3961 tcgagggcga gaaggtgacg gcgtcggcgg gcgtgcccac ggtctggctc ggactcctcg
     4021 cgtacatgaa ggagaacaag ctccgcttcg gcacgctcaa gaccgtcgtc gtcggcggct
     4081 cggcgtgccc gccggcgatg atcaaggcct tccaggacga gtacggcgtc gcctgccgcc
     4141 acgcctgggg catgacggag atgagcccgc ttggcacctt ctactcgccg aagaaccggc
     4201 acgacgcgct ctcggccgac gcgcgccacc agctcgagtg caagcagggg cggacgatct
     4261 acggcgtgca gatgaagatc gtcgacgggt cgggcaagga gctgccgcgc gacggcaagg
     4321 ccttcggcga cctgctcgtg cgcgggccgt gggtgctgcg cgagtacttc aaggccgagg
     4381 acgatccctt gcgcgacggg tggttcccga ccggcgacgt cgcgaccatc gacgccgagg
     4441 gctacatgca gatcaccgac cgctcgaagg acgtcatcaa gtccggcggc gaatggatca
     4501 gctcgatcga cctcgagaac atcgcggtcg cgcaccccgc gatcgccgag gccgcggtga
     4561 tcggcgtgag gcacccgaaa tgggacgagc gcccgctcgt ggtcgcggtg aagaaggccg
     4621 gcgcgagcgt cacgcgcgag gagctgctcg ccttctacca gggcaaggtc gcgaagtggt
     4681 ggatgcccga cgacgtcgtg ttcgtcgagc agctgccgca caccgcgacg ggcaagctcc
     4741 tgaagacgcg gctgcggcag gacttcaagg accaccggct gccggccggc aaatagcgtg
     4801 gagccgatcc tcaccgggcg cgacggcggc gtgctgcgca tcgagctgaa tcgccccgag
     4861 aagaagaacg cgatcacgat cgcgatgtac caggcgctcg gcgacgcgct gcattcggcc
     4921 tccggcgatg gcgcggtgcg cgccgtgctc ctgcacggga agcccgaggt gttctgcagc
     4981 ggcaacgatg tcgccgattt cctcgccgcg ccgtctcgca gcgaggactt cgcgccgttc
     5041 cgcttcctgc gcatcctcgg acagttcgac aagccgctgg tcgccgcggt gagcggggcg
     5101 gcgatcggca tcggcacgac catgctgctg cactgcgacc tcgtctacgc cgccgcgggg
     5161 acgcgcttcc agctgccctt cgcgagcctc gggctcgtgc ccgaggcggc gtcgagcctc
     5221 ctgctgccgc ggcttgccgg atggcagaag gccgccgagc tgctgatgct cggggagccg
     5281 ttcggcgccg agaccgcgcg cgagatcggc ctggtgaacg agatcgtcgc gccgcaggcc
     5341 gtgctcgaga ccgcgcgcgc gaaggcgctc gcgctcgccg cgaagccgcc cgcggcgatg
     5401 cgcgcgacca aggcgctgat gaagcaggcc gtgctcgcgg agatcgagca gaccatgacg
     5461 cgcgaggtca agaccttcgg cgagcgcctg gtctcgcccg aggcgaagga ggcgctcagc
     5521 gccttcctcg agaagcgcaa gccggatttc tcgaagttca gctgacgatg accctgaagg
     5581 gcaagacgct gttcatcacc ggcgcgtcgc gcggcatcgg cctcgcgatc gcgctgcgcg
     5641 cggcgcgcga cggggcgaac gtcgtcgtcg ccgcgaagac caccgaggcg cacccgaagc
     5701 tgcccggcac catctatacc gccgccgagg agatcgagaa ggcgggcggc aaggcgctgc
     5761 cgatcgcgct cgacatccgc gacgacgcgg cggtgcagca ggcggcggcg aaggcggtcg
     5821 agaccttcgg cggcatcgac atcctcgtga acaacgcgag cgcgatcaac ctcaccggga
     5881 cgctcgcgac cccgatgaag cgcttcgacc tcatgttcgg ggtgaacgtg cgcggcacct
     5941 actgctgctc gcaggcctgc ctgccgtacc tgaagaaggc cgccaacccg catatcctga
     6001 ccctgtcgcc gccgctcaac atgaagccga aatggttcaa ggaccacgtc gcctacacca
     6061 tggcgaagta cggcatgagc atgtgcgtgc tcggcatggc cgaggagttc cgcgccgacg
     6121 gcgtcgcggt gaacgcgctg tggccgcgca ccgtgatcca gacggctgcg atcgcgatgc
     6181 tcggggggct ggtgaagccc gaaaactgca ggaaacccga gatcgtggcc gacgcggctt
     6241 acatcatcct gaatcgagac agccgccgct atacgggcca cttcgccatc gacgacgagg
     6301 tgcttgcggc cgagggcgtg accgacttgg agcgctacgc gctggtcccg ggcaaggctc
     6361 gcttgctgcc ggatttcttt ctggattgac aatgggagca caatagggac gccccggggt
     6421 ggtatcaact agggagggat gccatgaagc gaatgatcgc agtggtcggg gggctgtgct
     6481 tgctcgcggg ctgcacagcg atgactacga tgaccgggac ccaatccgac gtgtccatcc
     6541 aagtagcgga gcgtacgtat agcagtgcac ccgcgacgga caccttcggc gccacgagct
     6601 tcgggaatta cgaattcaag gcatcccggc ccggtcagga gcccgtgtac gg
//
LOCUS       DATDMW010000075         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_394107, whole genome shotgun sequence.
ACCESSION   DATDMW010000075 DATDMW010000000
VERSION     DATDMW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394107"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VI319_03340"
     CDS             complement(<1..562)
                     /locus_tag="VI319_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6862910.1"
                     /translation="MNVRRPSLLLALAAAAALALPGAASAQARRPAGGTGFGILIGFE
                     DSKYDNGLALRLDGEIPLQSVSPVVRLSLVGSLGFTRFSFNPGFFGGESETLNIFKVT
                     PALRFTFGNHPLIRPYADAGIGLHYARISAPCAFDQFGNVVTCSDSDVSAHLRFAGGL
                     TFQVSPGLGLGAEVDLIPYFGKVDDNT"
     gene            complement(640..1986)
                     /locus_tag="VI319_03345"
     CDS             complement(640..1986)
                     /locus_tag="VI319_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6862911.1"
                     /translation="MDPKLLLKSRRIATLSVLSAASGMPYGWVVSGTLQAFLVALGFT
                     RSSIGLLSGVSLPWALKFLWSPLVDRYALRWPGRRRSWMIMAQLALAGTMGAVAAFAW
                     RALAARSAGGMVPHAALLIGLLALTFAFFSATQDIAYDAYTVEVLHRDEQGPVSGLRI
                     LFYRLGMLLSSAAAIGASEWIPWPIVFLALGGIFVLFTLLVLAAPEPDRPAEPPRSLA
                     RAVVDPLRSYFSRSDAIPIALFLVFYKFGDNMGGTMVIPFLKDLCFSNAELGAALKTI
                     GTIATISGSLLGGFLLSRMGLGRALWIFGVLQAGANVVYSGAALSRHVPLEFAQCASL
                     PAVDAATRLWTYGAISAEYFAHGMATAAQAVLLLRVCDKRYSATQFALLSSLFGLGRW
                     AAGLPSGFFVERMGYPAFFAMCATVMALPGFFFLHRIAPFAEREVRLAGDAAAHPQ"
     gene            complement(2031..>2319)
                     /locus_tag="VI319_03350"
     CDS             complement(2031..>2319)
                     /locus_tag="VI319_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HEY6862912.1"
                     /translation="PFACRVEEVLDALASSRGAVEVNGDPKRLELEPRWLRAATARGI
                     PVVLSVDAHSIRDLGYLRYAVGVARRGWVRRGQVLNTLPLDRFKAAVRPVA"
BASE COUNT          406 a          796 c          868 g          249 t
ORIGIN      
        1 acgtgttgtc gtcgaccttc ccgaagtacg ggatgaggtc gacctccgcg ccgaggccga
       61 gcccggggct cacctggaac gtgagcccgc cggcgaagcg caggtgcgcg ctcacgtccg
      121 agtcgctgca cgtgacgacg ttcccgaact ggtcgaatgc gcacggcgcg gagatccggg
      181 cgtagtggag gccgatgccc gcgtcggcat acggccggat gagcgggtgg tttccgaagg
      241 tgaatcgcag cgccggggtg accttgaaga tgttgagcgt ctcgctctcg cccccgaaga
      301 agcccgggtt gaagctgaag cgcgtgaacc cgagcgatcc gacgagcgac agccgcacca
      361 ccggcgagac ggactgcagc gggatctcgc cgtcgaggcg cagggcgagc ccgttgtcgt
      421 acttgctgtc ctcgaacccg atcaggatcc cgaatccggt gccgccggcg gggcggcgcg
      481 cctgggcgga cgcggcgcca ggcagcgcga gcgcggcggc agcggcgagg gcgagcagga
      541 gcgacgggcg gcggacgttc atgatcttcc tccgggcgcg cggggttcgg ggcgcggcgc
      601 gggccaacct aacacgagcc gtgcgagaga ccgcaagcat tactgtgggt gcgcagcagc
      661 gtccccggcg aggcgcacct ctcgctccgc gaagggcgcg atgcggtgca ggaagaagaa
      721 cccgggcagc gccatcacgg tcgcgcacat ggcgaagaag gcggggtacc ccatccgctc
      781 cacgaagaag ccggacggca ggccggcggc ccagcgcccc aggccgaaga gggaggagag
      841 gagcgcgaac tgtgtggcgc tgtagcgctt gtcgcacacg cgcaggagca gcaccgcctg
      901 cgccgccgtc gccatgccat gcgcgaagta ctcggcgctg atcgcgccgt acgtccacag
      961 ccgcgtggcg gcgtccacgg cgggcagcga cgcgcattgc gcgaactcga gcggcacgtg
     1021 ccgagagagc gccgcgccgg agtagacgac gttcgcgccg gcctggagca cgccgaagat
     1081 ccagagcgcg cgcccgagcc ccatccgcga caggaggaac cctccgagga gcgagcccga
     1141 gatcgtcgcg atcgtgccga tggtcttcag cgcggcgccc agctccgcgt tcgagaagca
     1201 caggtccttc aggaacggga tcaccatcgt cccgcccatg ttgtcgccga acttgtagaa
     1261 gacgaggaac agcgcgatgg ggatcgcgtc cgagcgcgag aagtacgagc ggagcggatc
     1321 caccaccgcg cgcgcgagcg atcgcggcgg ctcggcgggc cgatccggct ccggggcggc
     1381 gagcacgagc agcgtgaaca gcacgaagat cccgcccagc gcgaggaaga cgatcggcca
     1441 cgggatccac tcgctcgcgc cgatcgccgc ggcgctcgag agcagcatgc ccagccggta
     1501 gaagaggatg cgcagcccgg agaccggccc ctgctcgtcc ctgtgcagga cctcgaccgt
     1561 gtacgcgtcg tacgcgatgt cctgcgtggc ggagaagaac gcgaacgtga gcgcgagcag
     1621 gccgatgagg agcgccgcgt ggggcaccat cccgcccgcg ctccgcgccg cgagcgcgcg
     1681 ccacgcgaag gcggcgaccg cgcccatggt cccggcgagc gcgagctgcg ccatgatcat
     1741 ccagctccgg cggcggcccg gccagcgcag cgcgtaccga tcgacgagcg gggaccagag
     1801 gaacttgagc gcccagggca gcgacacgcc cgagaggagc ccgatggagg agcgcgtgaa
     1861 gccgagcgcg acgaggaacg cctggagcgt cccggagacg acccacccgt acggcatccc
     1921 cgacgcggcg gagagcacgc tcagcgtggc gatccgcctc gacttgagca ggagcttggg
     1981 atccaaggcg gtgcgagggt agacgcgggg gggcgcggcg acaacaccca tcaagcgacc
     2041 ggccgcacgg cggccttgaa ccggtccagc ggcagcgtgt tcagcacctg gccgcgccgc
     2101 acccacccgc gccgcgccac gccgaccgcg tagcgcaggt agccgaggtc gcggatcgag
     2161 tgcgcgtcga ccgagaggac cacggggatg ccccgcgcgg tggctgcgcg cagccagcgc
     2221 ggctcgagct cgagccgctt gggatcgccg ttcacctcga ccgcgccgcg cgacgaggcg
     2281 agcgcgtcga gcacctcctc cacccggcag gcgaagggg
//
LOCUS       DATDMW010000076         5999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_146466, whole genome shotgun sequence.
ACCESSION   DATDMW010000076 DATDMW010000000
VERSION     DATDMW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146466"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VI319_03355"
     CDS             <1..239
                     /locus_tag="VI319_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862913.1"
                     /translation="TALGATSIVVSYDVSEAVKLADHIYLLGDGVVAAQGMPEELAAS
                     RDPFVRQFMDAKPDGPIPFNVPAAPFAQQIGLAS"
     gene            255..2585
                     /locus_tag="VI319_03360"
     CDS             255..2585
                     /locus_tag="VI319_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6862914.1"
                     /translation="MGQFGIGQSVTRFEDPRLLRGEGRYIHDVNLPGQLHAVIVRSPH
                     AHAKIVSIDAKAAAAAPGVAAVWTGEDFAREGFGLPAPALKRTRPDGSPMFARAHPGL
                     ATGRVRHVGDPVAVVLAETLALAKDAAELLEVEYEDLPSVTSTAEAATPGAPRVWDEN
                     PDNISNVFELGNKAATDAAFAKAAHVVKRKYVITRVHAQYMETRGAVAAYEPAEGKYT
                     LYTDVQYPHRMRQFLAGTVFRIPESKVHVIAGDIGGAFGAKGPQYPEHRILMWAAKRT
                     RRPVKWVCERSEAVLADEHARDNVSEIELALGADGKFLGLRVSTLAALGAYVSSDRNL
                     LSTFQSVSTVVGTYTITAAHVRITGVMTNTNSTAPYRGAGRPEATYLIERIIDDAARE
                     LKLDRVELRRKNLIPPDAFPWKTPLAYTYDCGEFARGMDKAVQLADVAAFARRKAESE
                     RRGRLRGLGIVNAIERASSPGLEYAEVRFHPGGTATLAMGSKNQGQGHETSLRQILNE
                     RLGIDPKDVAYVDGDTEKVAFGVGTMGSRTMAIAGTAIVIASDKIIAKGRKIAAHLME
                     AAEPDVVFDAGKFKVAGTDKAVTLKDVAKAAYEPDKLPKGLEPGLTESGMFSPENHTF
                     PNGTHVCEVEIDPDTGVVAVERYTVVDDVGTVVNPLTLKGQIHGGVVQGLSQVLFERI
                     EYDPDNGQLLTGSFMDYAMPRADDVSNIEVGSNPVPTKLNPLGVKGAGEAGTVGALPA
                     VMNAIMDALAPVGVKELDMPATPDRVWRAIRSARGA"
     gene            complement(2582..3025)
                     /locus_tag="VI319_03365"
     CDS             complement(2582..3025)
                     /locus_tag="VI319_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6862915.1"
                     /translation="MFSEPIGNVMERKLLTAPPEITVGEAARLMAKRKVGAVLVVGED
                     RRLIGIFTERDAVFRVIARGLNPRTTPLADAMTRSPLTVHPGKSLGYALLMMHDHGFR
                     HAPVVEDGKLVGIVSARSALDPDLEEFVAEAQRREQIRREGEAHP"
     gene            3163..4065
                     /gene="lepB"
                     /locus_tag="VI319_03370"
     CDS             3163..4065
                     /gene="lepB"
                     /locus_tag="VI319_03370"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY6862916.1"
                     /translation="MDPTEAYRRPSKRVAAALGLLVPPAGMLYVARPGWAGIYLAWAL
                     FIGAVSLFVPREREWIVGAVVVLVGIVCAVQAYRYCRDSRVLRRGWYARWPGLLAIVA
                     AFAALAIGARAFLVEPFRAPSGSMLPSIVPGARLIVKKWGYGNYGALGIHFARNGISA
                     GLSRGDIVVFEYPEDTTVIYAKRIVGLPGDRVAYFSKRLWINDQEVPRSPVAGSPLEY
                     VERLGGREYPVLIEPGAPVSVLAARPFPFRDRCTFTAEGLSCPVPEGHYFVLGDNRDN
                     SSDSRVWGFVPARNIVGVVAYILP"
     gene            complement(4067..4855)
                     /locus_tag="VI319_03375"
     CDS             complement(4067..4855)
                     /locus_tag="VI319_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6862917.1"
                     /translation="MKEARVDFADAGAYERFMGRWSRAVAPQFLAWLGPKPRARWLDV
                     GCGTGILAEALLDLCEPASVTGIDASAAQVAQAARGPARERAKFRQADAAELPFADAA
                     FDIAVCALTLNFVPDPLRALREMRRVTVPGGAIGAYVWDFGRELSPSGPLREAMRAFG
                     AEVPAIPGTAHSGLDALESLFARAGLRSIETRTIDVTLAYSGFEDFWQAQTPGYSPTA
                     KIVDAMAEGERRRLKRAVQEALPTGPDGRIEYAARANAVRGLAP"
     gene            complement(4858..5823)
                     /locus_tag="VI319_03380"
     CDS             complement(4858..5823)
                     /locus_tag="VI319_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862918.1"
                     /translation="MKISGFMLGLLALLMAGSAAAQTHYPEKPIHLVVGFPPGSPPDT
                     FARLFGQRLTGTWSEPVLIENVTGAAGNIAADRVAKAAPDGYTLGVLTEAQIVVNPGL
                     YALAYDPVRDFAPVSQLFASPNLLVVSNAVPAKSLEELVALAKAQPGALTFVSGGSGS
                     TAHMAGELFKSAAGIDIRHVPYKGVLAAMPDLLGGRVTMVFSPMAIVLPMVREGKLRA
                     LAVTSLKRSAAAPELPTIAESGYPGFEVAGWNGLLAPAKTPAAVVRALNVEAVKALAH
                     PEIRAKLDGLGLQAIGSSPDQFAAVIASQIPKWTKVIRDAGIKAD"
BASE COUNT          913 a         2070 c         2157 g          859 t
ORIGIN      
        1 acacggcgct cggcgcgacc tcgatcgtgg tgagctacga cgtgagcgag gcggtgaagc
       61 tcgccgacca tatctatctg ctcggcgacg gcgtggtcgc ggcgcagggc atgcccgagg
      121 agctggccgc gtcgcgcgat ccgttcgtgc gccagttcat ggacgcgaag cccgacgggc
      181 cgatcccgtt caacgtgccg gcggcgccgt tcgcgcagca gatcggtctc gcttcctaat
      241 cacaagaaag caccatgggt cagttcggca tcggccagtc cgtcacgcgc ttcgaggatc
      301 cgcgcctgct gcgcggcgag ggccgctata tccacgacgt gaaccttccc gggcagctgc
      361 acgcggtgat cgtgcgctcg ccgcacgcgc acgcgaagat cgtctcgatc gacgccaagg
      421 cggcggccgc ggcgcccggc gtggcggcgg tgtggaccgg cgaggacttc gcgcgcgagg
      481 gcttcggcct gcccgcgccg gcgctcaagc gcacgcggcc cgacggctcg ccgatgttcg
      541 cgcgcgcgca tccgggcctc gcgaccgggc gcgtgcgcca cgtcggcgac ccggtggcgg
      601 tggtgctcgc cgagacgctc gcgctcgcga aggacgcggc cgagctgctc gaggtcgagt
      661 acgaggacct gccgtcggtg acctcgaccg cggaggcggc gacgcccggc gctccgcgag
      721 tgtgggacga gaacccggac aacatctcga acgtgttcga gctcggcaac aaggccgcga
      781 ccgacgcggc gttcgcgaag gccgctcacg tggtcaagcg caagtacgtg atcacgcgcg
      841 tgcacgcgca gtacatggag acgcgcggcg cggtggcggc ctacgagccc gccgagggca
      901 agtacacgct gtacaccgac gtgcagtatc cgcaccgcat gcgccagttc ctcgccggga
      961 cggtgttccg cattcccgag agcaaggtgc acgtcatcgc cggcgacatc ggcggcgcgt
     1021 tcggcgcgaa ggggccgcag tacccggagc accgcatcct gatgtgggcg gcgaagcgca
     1081 cgcgccggcc ggtgaagtgg gtgtgcgagc gcagcgaggc ggtgctcgcc gacgagcacg
     1141 cgcgcgacaa cgtgagcgag atcgagctcg cgctcggcgc cgacgggaag ttcctcgggc
     1201 tgcgcgtgag cacgctcgcc gcgctcggcg cctacgtgtc ctcggaccgc aacctcctct
     1261 ccacgttcca gagcgtgagc acggtggtcg ggacctacac gatcacggcg gcgcacgtgc
     1321 ggatcacggg cgtgatgacg aacacgaact cgacggcgcc ttaccgcggc gccggcaggc
     1381 cggaggcgac ctatctcatc gagcgcatca tcgacgacgc ggcgcgcgag ctgaagctcg
     1441 accgcgtcga gctgcgccgg aagaacctga tcccgccgga cgcgttccca tggaagacgc
     1501 cgctcgccta cacctacgac tgcggcgagt tcgcgcgcgg catggacaag gcggtccagc
     1561 tcgcggacgt cgcggccttc gccaggcgca aggccgaatc ggagcggcgc ggaaggctgc
     1621 gcggcctggg catcgtcaac gcgatcgagc gcgcgtcctc gccgggcctc gagtacgcgg
     1681 aggtgcgctt ccacccgggc ggcacggcga cgctcgcgat ggggagcaag aaccagggcc
     1741 agggccacga gacgagcctg cggcagatcc tgaacgagcg gctggggatc gacccgaagg
     1801 acgtcgccta cgtcgacggc gacaccgaga aggtcgcgtt cggcgtcggc accatgggct
     1861 cgcgcaccat ggcgatcgcg gggacggcga tcgtcatcgc ctccgacaag atcatcgcca
     1921 agggcaggaa gatcgcggcg cacctgatgg aggccgccga gcccgacgtc gtcttcgacg
     1981 ccgggaagtt caaggtggcg ggcaccgaca aggcggtcac gctgaaggac gtggcgaagg
     2041 ccgcctacga gcccgacaag ctgccgaagg gcctcgagcc cgggctcacc gagagcggca
     2101 tgttctcgcc ggagaaccac accttcccga acgggacgca cgtgtgcgag gtcgagatcg
     2161 accccgacac gggcgtggtc gcggtcgagc gctacaccgt cgtcgacgac gtcggcaccg
     2221 tcgtcaatcc gctcacgctc aagggccaga tccacggcgg ggtggtgcag ggcctgtcgc
     2281 aggtgctgtt cgagcgcatc gagtacgacc ccgacaacgg ccagctgctg accggctcgt
     2341 tcatggacta cgccatgccg cgcgcggacg acgtgtcgaa catcgaggtc ggcagcaacc
     2401 cggtgccgac gaagctcaat cccctgggcg tgaaaggcgc gggcgaggcg ggcaccgtcg
     2461 gcgcgctgcc cgcggtgatg aacgcgatca tggacgcgct ggcgccggtc ggggtgaagg
     2521 agctcgacat gcccgcgacg cccgaccgcg tgtggcgggc gatccggagc gcgcgcggcg
     2581 cctaagggtg cgcctcgcct tcgcggcgga tctgctcgcg cctctgcgcc tccgccacga
     2641 actcttccag gtcgggatcg agcgcgctgc gcgcggagac gatgccgacc agcttgccgt
     2701 cctcgaccac cggcgcgtgg cggaagccgt ggtcgtgcat catgagcagc gcgtacccga
     2761 gcgacttgcc cggatgaacg gtgagcggcg agcgcgtcat cgcgtcggcc agaggcgtgg
     2821 tgcgcgggtt caggccgcgc gcgatcacgc ggaacaccgc gtcgcgctcg gtgaagatgc
     2881 cgatcagcct gcggtcctcg ccgacgacca gcaccgcgcc gaccttgcgc ttcgccatca
     2941 gtctcgccgc ctcgccgacc gtgatctccg gcggagcggt gaggagcttg cgctccatga
     3001 cgttgccgat gggctcgctg aacatggtcc ttactccccg ttgcctaccg cgcacgctaa
     3061 ccgcgattcg gattcgcgga ttgacgcagg tcaagcccgg ctatgcggcc ggggcgcccg
     3121 gctgcgacaa taggcggcga aagggggacg tgggcggcgg cgatggaccc gaccgaggca
     3181 taccgaaggc cgagcaagcg cgtcgccgcc gcgctcggac tgctcgtccc gccggcgggc
     3241 atgctctacg tcgcccgtcc cggctgggcg ggaatctatc tggcgtgggc gcttttcatc
     3301 ggcgcggtca gcctgttcgt gccgcgcgag cgcgaatgga tcgtcggcgc cgtcgtcgtg
     3361 ctcgttggaa tcgtctgcgc ggtgcaggcc taccgctact gccgcgactc gcgcgtgctc
     3421 cggcgcggct ggtacgcgcg ctggcccgga ttgctcgcga tcgtcgcggc gttcgcggcc
     3481 ctggcgatcg gggcgcgggc gttcctggtc gagccgtttc gcgccccgtc cggatcgatg
     3541 ctgccgtcga tcgtcccggg cgcgcggctg atcgtgaaga agtggggata cggcaactac
     3601 ggcgccctgg gaatccactt cgcgaggaac ggcatctccg cgggcctgag ccgcggagac
     3661 atcgtcgtgt tcgagtatcc ggaggacacg acggtcattt atgcgaagcg gatcgtcggg
     3721 ctgcccggcg accgggtcgc ctatttcagc aagcgcctct ggatcaacga ccaggaggtg
     3781 ccgcggagcc cggtcgccgg ctccccgctg gagtacgtcg agcggctcgg cgggcgcgag
     3841 tacccggtcc tcatcgagcc cggcgcgccg gtttccgtgc tcgcggccag gccgtttccc
     3901 ttcagggacc gatgcacctt caccgccgag ggcctgtcct gcccggtgcc cgagggccac
     3961 tacttcgtcc tgggcgacaa ccgcgacaac agctcggaca gccgcgtctg gggcttcgtg
     4021 ccggcgagga acatcgtcgg cgtggtggcg tacatcctgc cctagctcac ggggcgaggc
     4081 cgcggacggc attcgcgcgc gcggcgtact cgatcctccc gtccgggccg gtcggcagcg
     4141 cttcctgcac cgcgcgcttc aggcgccggc gctcgccttc ggccatggcg tcgacgatct
     4201 tcgcggtcgg gctgtacccc ggcgtctgcg cctgccagaa gtcctcgaag ccggagtagg
     4261 cgagcgtcac gtcgatggtt ctcgtctcga tcgaccgcag cccggcgcgc gcgaagagcg
     4321 actcgagcgc gtcgagtccc gaatgcgcgg tgcccgggat cgccggcacc tcggcgccga
     4381 acgcgcgcat cgcctcgcgc agcggcccgc tcggcgagag ctcgcgtccg aaatcccaga
     4441 cgtaggcgcc gatcgcgccg cccgggacgg tgacgcgccg catctcgcgc agcgcgcgca
     4501 ggggatccgg gacgaagttc agggtgagcg cgcagaccgc gatgtcgaag gcggcgtcgg
     4561 caaagggcag ctcggcggcg tccgcctgcc ggaacttcgc gcgctcgcgc gcggggccgc
     4621 gcgcggcctg cgcgacctgc gcggccgacg cgtcgatccc ggtcacggac gccggctcgc
     4681 agaggtcgag cagcgcctcg gcgaggatcc ccgtgccgca gccgacgtcg agccagcgcg
     4741 cgcgcggctt cggtccgagc cacgccagga actgcggcgc gacggcgcgg ctccagcgtc
     4801 ccatgaagcg ctcgtaggcg ccggcgtcgg cgaagtcgac gcgcgcttcc ttcatgtcta
     4861 atcggccttg atgcccgcat ccctgatcac cttcgtccac ttcgggatct gggacgcgat
     4921 gaccgcggcg aattgatcgg gagagctgcc gatggcttgc agcccgaggc cgtccagctt
     4981 ggcgcgtatc tcgggatgcg cgagcgcctt gacggcttcg acattgagcg cgcggacgac
     5041 cgccgccggc gtcttcgcgg gcgcgagcag cccgttccag cccgccacct cgaatcccgg
     5101 atagcccgac tcggcaatgg tcggcagctc cggcgccgcc gcggagcgct tcagcgaggt
     5161 caccgcgagc gcgcgcagct tcccttcgcg caccatcggc aggacgatcg ccatcgggct
     5221 gaagaccatc gtgacgcgcc cgccgagcag gtcgggcatc gccgccagca cgcctttgta
     5281 ggggacgtgc cggatgtcga tgccggccgc ggacttgaac agctcgccgg ccatgtgcgc
     5341 cgtgctgccg ctgccgccgg agacgaaagt gagcgcgccc ggctgcgctt tggccagggc
     5401 gaccagctcc tccaggctct tcgcgggcac cgcgttgctc acgaccagga ggttcgggct
     5461 ggcgaagagc tgggagacgg gcgcgaaatc ccggacgggg tcgtacgcga gcgcatacag
     5521 gccgggattg acgacgatct gcgcctcggt caggacgccc agggtgtagc cgtcgggcgc
     5581 ggccttcgcc acgcggtcgg ccgcgatatt gccggccgcg ccggtgacgt tctcgatcaa
     5641 taccggctcg ctccacgtcc cggtgagcct ctggccgaac aggcgcgcga aggtgtccgg
     5701 cgggctgccg ggcggaaagc cgacgacgag gtgaatgggc ttctcggggt aatgcgtctg
     5761 cgcggccgcg ctgcccgcca tcagcagcgc cagcaggccg agcatgaaac cggagatctt
     5821 catcgctgct cctcggttcg tgtggcgcca aagttagcga aggccgagag ataagacaaa
     5881 tgaattgtta gaattcgtcg cattccggtc attgatgtca tatccggccc gggaggaccc
     5941 agcatgaaat cgcggatcga caacatcgga cggcgcctcg acctgaattt gctggtcac
//
LOCUS       DATDMW010000077         7879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_146768, whole genome shotgun sequence.
ACCESSION   DATDMW010000077 DATDMW010000000
VERSION     DATDMW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146768"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /gene="glpK"
                     /locus_tag="VI319_03385"
     CDS             <1..1286
                     /gene="glpK"
                     /locus_tag="VI319_03385"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY6862919.1"
                     /translation="PERVAAIGIANQRETAILWDRRTGRPAHHAIVWQDRRTADACER
                     LKREGAADAVRAKTGLVIDPYFSATKIAWMLDRVPGLRARAERGDVAFGTVDTWLAWK
                     LSGGRLHVTDPSNASRTLLYDIHRGDWDDELLAIFRVPRAVLPEVKPSSFVYGASAKA
                     LLGAEIPIAGIAGDQQAALFGQACHEPGMAKNTYGTGCFLLLNTGSEAIASKNGLLTT
                     SAAQAGAREYALEGGVFIAGAVVQWLRDGLKLIRRSADVERLAASVRDSGGVYFVPAF
                     AGLGAPHWDPYARGVIVGLTRGSSAGHVARAALESIAFQSAELLEAMRKDSGVALREL
                     RVDGGAAATDMLMQFQADLLGVPVVRPRVLETTALGAAYLAGLATGVWESRADIARHW
                     KAAKRFEPRMKRDEAARRMHEWSRALERSRGWEQS"
     gene            1301..1648
                     /locus_tag="VI319_03390"
     CDS             1301..1648
                     /locus_tag="VI319_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6862920.1"
                     /translation="MDIKRHEPSKILSEAVEHGNTVYLAGIVADDPSQDVKGQTKQIL
                     DKIDRLLATCGSHKSKVLSANIWVTDIKNRPAMNEVWTAWVDPKNLPARACVEAKLAD
                     PRMLVEIMVVAGK"
     gene            complement(1691..3136)
                     /gene="tldD"
                     /locus_tag="VI319_03395"
     CDS             complement(1691..3136)
                     /gene="tldD"
                     /locus_tag="VI319_03395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY6862921.1"
                     /translation="MNAPEQVFRTAQSFLLAPHDLEARNLGRVFGALLTHRLDYADLY
                     FQYARSEGWSLEEGIVKSGSFSIDQGVGVRAISGEKTAFAYSDEISMPALEDAARATR
                     AIARSGGKGKPVAVRQGTGLALYQPLDPLASLPDTEKVKLLEKLERLCRDLDPRVVQV
                     MASLAGEYEVVLVARSDGVLSADVRPLVRCSVQVIAEQNGRREQGSAGGGGRTDYSWF
                     TDERLADYAKKAVDQALVNLESRPAPAGTMTVVLGPGWPGVLLHEAIGHGLEGDFNRK
                     GSSAFTGRIGQKVAARGVTVVDDGTLPGRRGSLNIDDEGNPTQCNTLIEDGVLRAYMQ
                     DSLNARLMGVPVTGNGRRESFAHVVLPRMTNTYMLNGDKDPGEIVASVKKGLYAVNFG
                     GGQVDITNGKFVFSASEAYMIEDGKVTHPVKGATLIGNGPDALTRVSMVGNDLALDTG
                     VGTCGKDGQSVPVGVGQPTLRIDGLTVGGTG"
     gene            complement(3167..4030)
                     /locus_tag="VI319_03400"
     CDS             complement(3167..4030)
                     /locus_tag="VI319_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY6862922.1"
                     /translation="MSAAPGFKVAAVQMVSTHVVAENLAVAAELVARAAAQGAGLVAL
                     PEYFCIMGMNERDKVAVKEADGDGPIQAFLAETAARHGVWLVGGSVPLATGRPDKVRN
                     SCLVYGPDGRRTARYDKMHLFGLELGAERFDEANTIEPGERPLAIDSPYGRIALSICY
                     DVRFPELYRSLGDVDLILVPSAFTATTGEAHWETLLRARAIENLAWVVAPAQGGTHPS
                     GRRTHGHSMVIDPWGAVRGCLPQGAGVVVAAVDPGLQARLRASLPALEHRRIGAEAEP
                     LEGARPRRNVP"
     gene            complement(4027..>7879)
                     /locus_tag="VI319_03405"
     CDS             complement(4027..>7879)
                     /locus_tag="VI319_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEY6862923.1"
                     /translation="RLPGTLVHVEESIAKLAQRGSRWRSLARTAGWTAVVLYFVFALL
                     VLALRYWLLPLAGSHTDLIERKASRMLGERVTIGAIEAGWQGLRPELMLSNVTVYDRS
                     GRPALTLPEIDATVSWSSLARLSLHFYSLVLERPDLQILKNADGTLLVAGIALRGDAS
                     GDSGALDWLLDQREVVIRNGVLSWEDQQRGTPRLVLPEVSIVLRSGLTTHRFALRAKP
                     PAELGSALDLRGEFDAGDRYRFRTWSGRVYAELEYADLGAWSQWIRYPIAVESGRGGV
                     RTWLGFADGKLSELTADLALADVRARLASDLPQLRLSSLEGRLALRQSTRAIDASARR
                     IALRTEDGLAIEPTDFSAHWEPPAPGRAGRAEFQTAALRLEPLVQLAEFLPLPADARA
                     QLAAIEPRGTVRDLKLAWTGDSEHTERYSVRGGFAGLGARAWRSMPGFSGLTGQVDAN
                     ERGGRLGLASEHAVLQVDRLLHESEVRFDRLAADLEWRNAQDHVEVRIGSVAVSNPDL
                     TGSLTGTFATRAGSPGVVDLTGNFSRVDGQAVARYVPKLPPVVAEYLRAAVRRGGSDL
                     VRVRVKGDLADFPFPDPGKGTFQILVRLKNVDFRFAEDWPQLSDFSGELEFSGRSMRV
                     SSSQLTIEGVRIKSARAVVPDLFNGNEHLRIDGEAEEATAGFLRFVESSPVSRMVDGF
                     TRGIRATGNGRLELRLDIPIRHLNEVKVAGAYQFLGDQIRLSEQLPPLGQVTGRLEFT
                     EAGVNARGISGQFIGGPMSISAVTRDGIITVSAAGTAQAAGLPAGLADPWRKYVSGST
                     GWQASIIAGRARPTTITVESQLAGLALDLPAPLAKRPAEVLPLRFERVIGPEGAGGRR
                     SEQMKLTLGRGVNAQILGTRQGDQFVLGRAAVGFNEPALLPERDGVSVTGNFPYADVD
                     RWRAVLPESSKGGRVGPVQVALRIGALDFLGRRFNDVSVRTRTVGSAWSGTVAAKQFD
                     GDITWRPEDGGRIVARLKHFTVPDSAPGTTPSAETPSREMPALDVVVDSFTLRGLDLG
                     RLEVVAVNQAGQDWRIEKLVVANDDSTLSATGTWQSWKEKPTLNADIRLEVSDVGRYL
                     ERIGYPGTMRRGTATLEGKLSWEGAPSAVDYPTLSGNLKLTAAKGQFLRADPGVAKLL
                     GILSLQSWATLDVRNTFEKGFTFDTVSSTATLAKGVLTTGDFHMRGPAAQVNMKGTVD
                     LVHETQDLYLRVVPSLGDAASVYAILVNPVWGIPILVLQRILKDPLGQILGLEYHATG
                     SWEKPQVERVRADVKSAEANP"
BASE COUNT         1116 a         2828 c         2815 g         1120 t
ORIGIN      
        1 ggcccgagcg cgtcgccgcg atcggcatcg ccaaccagcg cgagaccgcg atcctctggg
       61 accggcgcac cggccgtccc gcgcatcacg cgatcgtgtg gcaggaccgg cgcaccgccg
      121 acgcgtgcga gcggctgaag cgcgaaggcg ccgccgacgc ggtgcgcgcg aaaacgggcc
      181 tcgtgatcga cccctacttc tccgcgacca agatcgcgtg gatgctcgac cgcgtgccgg
      241 ggctgcgcgc gcgcgccgag cgcggcgacg tcgccttcgg cacggtcgac acctggctcg
      301 cgtggaagct ctcgggcggc cggctgcacg tgaccgatcc gagcaacgcc tcgcgcacgc
      361 tgctctacga catccaccgc ggcgactggg acgacgagct gctcgcgatc ttccgcgtcc
      421 cgcgcgcggt gctgcccgag gtcaagccct cctccttcgt ctacggcgcg agcgccaagg
      481 cgctgctcgg cgccgagatc ccgatcgccg gcatcgcggg cgaccagcag gcggcgctct
      541 tcggccaggc gtgccacgag ccgggcatgg cgaagaacac ctacggcacg ggctgctttc
      601 tcctgctcaa caccggctcc gaggcgatcg cgtcgaagaa cgggctgctc acgacgagcg
      661 ccgcgcaggc gggcgcgcgc gagtacgcgc tcgagggcgg cgtcttcatc gcgggcgccg
      721 tcgtgcagtg gctgcgcgac ggcctgaagc tgatccggcg atctgccgac gtggagcggc
      781 tcgcggcgag cgtgcgcgac tcgggcggcg tgtatttcgt tcccgcgttc gccggcctcg
      841 gcgcgccgca ctgggacccg tacgcgcgcg gcgtcatcgt cggcctcacg cgcggatcga
      901 gcgccgggca cgtcgcgcgc gcggccctcg agtcgatcgc gttccagtcg gcggagctcc
      961 tcgaggcgat gcgaaaggac tcgggcgtcg cgctgcgcga gctgcgcgtc gacggcggcg
     1021 cggcggcgac cgacatgctc atgcagttcc aggcggacct gctcggcgtg ccggtcgtgc
     1081 gcccgagggt gctcgagacg acggcgctcg gcgcggccta tctcgccggg ctcgcgaccg
     1141 gcgtgtggga gagccgcgcg gacatcgcgc gccactggaa ggccgcgaag cgcttcgagc
     1201 cgcgcatgaa gcgcgacgag gcggcgcggc gaatgcacga atggtcgcgg gcgctcgagc
     1261 gctcccgcgg ctgggaacaa tcctgaccct ggagacccgc atggacatca agcgccacga
     1321 gccctcgaag atcctctcgg aagccgtcga acacggcaat accgtctacc tcgcgggcat
     1381 cgtcgccgac gacccgtcgc aggacgtgaa aggccagacg aagcagatcc tcgacaagat
     1441 cgaccgcctg ctcgcgacgt gcggcagcca caagtcgaag gtgctctcgg cgaacatctg
     1501 ggtgaccgac atcaagaacc gcccggcgat gaacgaggtc tggaccgcgt gggtcgatcc
     1561 gaagaacctg cccgcgcgag cctgcgtcga ggcgaagctc gccgatccgc gcatgctggt
     1621 cgagatcatg gtcgtcgccg gcaaatgaag ccgttcgcgc aggccggccg cgcgcgcgaa
     1681 ctgagccggc tcagcccgtc ccgccgaccg tcagcccgtc gatcctgagc gtcggctggc
     1741 ccacgccgac cgggaccgac tgcccgtcct tgccgcaggt ccccacgccc gtgtcgagcg
     1801 cgaggtcgtt gccgaccatg gacacgcgcg tcaacgcgtc cggcccgttg ccgatcagcg
     1861 tcgcgccctt cacggggtgc gtgaccttgc cgtcctcgat catgtaggcc tcggacgccg
     1921 agaacacgaa cttgccgttc gtgatgtcga cctgcccacc gccgaagttg acggcgtaga
     1981 ggcctttctt caccgaggcg acgatctcgc ccgggtcctt gtcgccgttc agcatgtaag
     2041 tgttggtcat gcgcggcagc acgacgtgcg cgaaggactc gcgccggccg ttgcccgtga
     2101 cgggaacgcc catgaggcgc gcgttcaggc tgtcctgcat gtaggcgcgc agcacgccgt
     2161 cctcgatcag ggtattgcac tgcgtcgggt tgccctcgtc gtcgatgttg agcgagccgc
     2221 ggcgcccggg tagcgtgccg tcgtcgacca cggtcacgcc gcgcgcggcc actttctggc
     2281 cgatgcgccc ggtgaacgcc gacgagccct tgcggttgaa gtcgccctcg aggccgtggc
     2341 cgatcgcctc gtgcagcagc acgccgggcc agccgggccc cagcaccacg gtcatcgttc
     2401 ccgccggggc ggggcgcgac tcgaggttga cgagcgcctg gtcgaccgcc ttcttcgcgt
     2461 aatcggcgag gcgctcgtcg gtgaaccagg agtagtcggt gcgcccgccg ccgccggcgc
     2521 tgccctgctc gcgccggccg ttctgctcgg cgatcacctg caccgagcag cgtacgagcg
     2581 gccgcacgtc ggcgctgagc accccgtcgg agcgcgcgac cagcaccacc tcgtattcgc
     2641 cggcgaggct cgccatcacc tgcacgaccc gcgggtcgag atcgcggcac aggcgctcga
     2701 gcttctcgag cagcttcacc ttctcggtat cgggcagcga cgcgagcggg tcgagcggct
     2761 ggtacagcgc gagcccagtc ccctggcgca ccgcgaccgg cttgcccttg ccgcccgagc
     2821 gcgcgatcgc gcgcgtcgcg cgcgcggcgt cctcgagcgc gggcatgctg atctcgtcgg
     2881 agtacgcgaa cgccgtcttc tcgccgctga tcgcgcgcac gccgacgccc tggtcgatcg
     2941 agaagctgcc ggacttgacg atcccttcct ccaggctcca gccctcgctg cgcgcgtact
     3001 ggaaatacag gtcggcatag tccagacggt gggtgagcag cgcgccgaag acgcgtccga
     3061 ggttgcgcgc ctcgagatcg tgcggcgcga ggagaaacga ctgggcggtg cggaagacct
     3121 gttcgggggc gttcatgcgg cgagttcctg acgcgactgt cccattctac gggacattgc
     3181 gccggggacg cgctccttca agtggctcgg cctcggcgcc gatcctgcga tgctcgagcg
     3241 cgggcaggct cgcgcgcagg cgcgcctgca gtcccgggtc caccgccgcc accaccacgc
     3301 cggcgccttg cggcaggcag ccgcgcaccg cgccccaggg atcgatcacc atgctgtgtc
     3361 catgcgtccg gcgaccgctc gggtgcgtgc cgccctgcgc cggcgcgacg acccaggcga
     3421 ggttctcgat ggcgcgcgcg cgcagcagcg tctcccagtg cgcctcgccc gtggtcgcgg
     3481 tgaacgccga cggcaccagg atgagatcga cgtcgccgag cgagcggtac agctcgggga
     3541 agcgcacgtc gtagcagatc gacagcgcga tgcgtccgta aggcgagtcg atcgcgagcg
     3601 gccgctcgcc gggctcgatc gtgttggcct cgtcgaagcg ctcggcgccg agctcgaggc
     3661 cgaagaggtg catcttgtcg tagcgcgcgg tacggcggcc gtcgggcccg tagacgaggc
     3721 agctgttcct caccttgtcc ggacgtccgg tcgcgagcgg caccgagccg ccgacgagcc
     3781 acacgccgtg acgcgcggcc gtttccgcga ggaaggcctg gatcggcccg tcgccgtcgg
     3841 cctccttcac ggcgaccttg tcgcgctcgt tcatccccat gatgcagaag tactcgggca
     3901 acgccaccag gccggcgccc tgcgcggcgg cgcgcgcgac gagctcggcg gcgacggcga
     3961 gattctcggc cacgacgtgc gtcgacacca tctgcaccgc ggcgaccttg aatcccggcg
     4021 cggcgctcac gggttcgcct cggcgctctt cacgtcggcg cgcacgcgct cgacctgcgg
     4081 cttctcccac gatccggtcg cgtggtactc gagaccgagg atctggccga gcggatcctt
     4141 gaggatgcgc tggagcacga ggatcgggat gccccatacc gggttgacga ggatggcgta
     4201 gaccgacgcg gcgtcgccga gcgaaggcac gacgcgcagg tagaggtcct gcgtctcgtg
     4261 cacgagatcc acggtcccct tcatgttcac ctgcgcggcc gggccgcgca tgtgaaagtc
     4321 cccggtcgtg aggacgcctt tcgcgagcgt cgccgtgctc gacacggtgt cgaaggtgaa
     4381 gcccttctcg aaggtattgc gcacgtcgag cgtcgcccag gactgcaggc tcaggatgcc
     4441 gagcagcttc gcgacgcccg ggtcggcgcg caggaactgt cccttggccg cggtaagctt
     4501 caggttgccg ctcagcgtgg gatagtcgac cgcgctcggc gcgccttccc aggacagctt
     4561 gccctcgagc gtcgcggtcc cgcggcgcat cgtgccggga tagccgatgc gctcgaggta
     4621 gcggccgacg tcgctcacct cgagccggat gtcggcgttg agcgtgggct tctccttcca
     4681 gctctgccag gtgccggtcg cgctgagcgt gctgtcgtcg ttggcgacca ccagcttctc
     4741 gatgcgccaa tcctgccccg cctggttcac ggcgacgacc tcgagtcgcc cgagatccag
     4801 gccgcgcagc gtgaagctgt cgacgacgac gtcgagcgcg ggcatctccc gcgacggcgt
     4861 ctccgcgctc ggcgtcgtcc cgggcgcgct gtcgggcacg gtgaagtgct tgaggcgcgc
     4921 cacgatgcgt ccgccgtcct cgggtcgcca ggtgatgtcg ccgtcgaact gcttcgcggc
     4981 cacggtgccc gaccaggcgc tgccgacggt gcgcgtgcgc acgctcacgt cgttgaagcg
     5041 ccgcccgagg aagtcgagcg cgccgatcct gagcgcgacc tgcaccggcc cgacgcggcc
     5101 gcccttcgac gattcgggga gcacggcacg ccagcggtcg acgtcggcat aagggaaatt
     5161 gcccgtgacg ctgacgccgt cgcgctcggg cagtagcgcg ggctcgttga atccgaccgc
     5221 cgcgcgcccg agcacgaact gatcgccctg ccgcgttccc aggatctgcg cgttcacgcc
     5281 gcggccgagc gtcagcttca tctgctcgct gcgccgcccg cccgcgccct cgggtccgat
     5341 cacccgctcg aagcgcagcg gcagcacctc ggcgggccgc ttcgcgagcg gcgcgggaag
     5401 atcgagcgcg agcccggcga gctgcgattc gacggtgatg gtcgtaggcc gtgcgcggcc
     5461 cgcgatgatg ctcgcctgcc agccggtgct gccgcttacg tacttgcgcc acggatcggc
     5521 gagccccgcg ggcagccccg ccgcctgcgc ggtgccggcc gcgctcaccg tgatgatgcc
     5581 gtcgcgcgtg accgcggaga tgctcatcgg tccgccgatg aactggcccg agatgccgcg
     5641 cgcgttcacg cccgcctcgg tgaactcgag gcggccggtc acctgaccga gcggcggcag
     5701 ctgctcgctg aggcggatct ggtcgcccag gaactggtag gcgcccgcga ccttgacctc
     5761 gttcaggtgc cggatcggga tgtcgagccg cagctccagg cgcccgttgc cggtggcgcg
     5821 gatgccgcgc gtgaagccgt cgaccatgcg gctcaccggg ctcgactcga cgaagcgcag
     5881 gaagcccgcg gtcgcctcct ccgcctcgcc gtcgatgcgc aggtgctcgt tgccgttgaa
     5941 gaggtcgggg acgacggcgc gcgcgctctt gatgcgcacg ccctcgatcg tgagctgcga
     6001 cgacgagacg cgcatcgagc gtccggagaa ctcgagctcg ccggagaaat cggagagctg
     6061 cggccagtcc tcggcgaagc ggaagtccac gttcttgagg cgcaccagga tctggaaggt
     6121 ccccttaccg gggtcgggga acgggaagtc ggccagatcg cccttgacgc gcacgcgcac
     6181 gaggtccgag cctccgcggc gcaccgcggc gcgcagatac tcggcgacca cgggcggcag
     6241 cttcgggacg taccgggcga cggcctggcc atcgacgcgc gagaaattgc ccgtgagatc
     6301 gacgaccccg ggactcccgg cgcgcgtcgc gaaggtgccg gtcagcgagc cggtgagatc
     6361 ggggttcgag acggcgacgc tgccgatgcg cacttcgacg tggtcctgcg cgttccgcca
     6421 ctccaggtcg gcggcgagcc ggtcgaagcg cacctcgctc tcgtgcagca ggcggtcgac
     6481 ctgcagcacc gcgtgctcgg aggcgaggcc gagacggccg ccgcgctcgt tggcgtcgac
     6541 ctgccccgtc aggcccgaga atcccggcat gctgcgccag gcgcgcgcgc cgaggccggc
     6601 gaagccgccg cgcaccgaat agcgctcggt atgctccgag tcgccggtcc acgcgagctt
     6661 gaggtcgcgc accgtcccgc gcggctcgat cgcggcgagc tgcgcgcgcg cgtcggccgg
     6721 cagcggcagg aactcggcga gctgcacgag cggctcgagc cgcagcgccg cggtctggaa
     6781 ctccgcccgg cccgctcgcc ctggcgccgg cggctcccag tgtgcgctga agtcggtcgg
     6841 ctcgatggcg aggccgtcct cggtgcgcag cgcgatccgg cgcgcgctcg cgtcgatcgc
     6901 ccgcgtcgat tgccggagcg cgagcctccc ctcgagcgag gacaggcgca gctgcggcag
     6961 gtcgctcgcg aggcgcgcgc gcacgtcggc gagtgcgagg tcggcggtca gctcggagag
     7021 cttgccgtcg gcgaagccca gccaggtgcg cacgccgccg cgtcccgact cgacagcgat
     7081 cgggtagcgg atccactggc tccaggcgcc gaggtccgcg tactcgagct cggcgtagac
     7141 gcgcccggac caggtgcgga agcggtagcg gtcgcccgcg tcgaattcgc cgcgcaggtc
     7201 gagcgccgag ccgagctcgg ccggcggctt ggcgcgcagc gcgaagcggt gggtggtgag
     7261 cccgctgcgc agcacgatgc tcacctcggg gagcaccagc cggggcgtgc ccctctgctg
     7321 gtcctcccag ctcagcacgc cattgcggat cacgacctcg cgctgatcga gaagccagtc
     7381 gagcgcgccc gagtcgcccg aggcgtcccc gcgcagggcg atgccggcga cgagcagcgt
     7441 gccgtcggcg ttcttcagga tctgcagatc ggggcgctcg agcacgagcg agtagaagtg
     7501 cagcgacagg cgcgcgagcg acgaccagga caccgtcgca tcgatctcgg gcagcgtcag
     7561 cgctggcctc cccgagcggt cgtatacggt gacgttggag agcatgagct cgggccgcag
     7621 cccctgccag cccgcctcga tcgcgccgat ggtgacgcgc tcgccgagca tccgcgaggc
     7681 cttgcgctcg atcaggtcgg tgtgggagcc ggcgagcggc agcagccagt agcgcagcgc
     7741 cagcaccagg agcgcgaaca cgaaatacag gaccaccgcg gtccagccgg ccgtccgggc
     7801 gagcgagcgc cagcggcttc cccgctgtgc gagcttcgcg atcgattctt cgacgtgcac
     7861 gagcgttccc ggaagcctg
//
LOCUS       DATDMW010000078         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_147498, whole genome shotgun sequence.
ACCESSION   DATDMW010000078 DATDMW010000000
VERSION     DATDMW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147498"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2168)
                     /locus_tag="VI319_03410"
     CDS             complement(<1..2168)
                     /locus_tag="VI319_03410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6862924.1"
                     /translation="MQQIAVNSRASITSGARAVAYRRRDLQDFLDRRQPRGDLHRPGD
                     AQRLHAFLERLLAQARAAEARVRGLTELSADWFWETDAEHRITWLSGGGPVATLFGGR
                     PTYGKRFWEIPRMEVDRRALDGLLERLEAEAPFFDLEIARTDDRGARQVHIISGQSRK
                     DAHGSFVGYRGVGRDVTEQRRAERGLAAAKERLEMALDGGNLAEWHLDLESDDLYAGD
                     GWVRFLGHDRSPPITRGSQLAGLVHADDAARLREATIRALKGLDAEYDVELRIRTREG
                     DWRWLHCRGRVTERDADGRALRMSGIVADIDARKRAEAAAAERDRRFRDVASVSREYV
                     WEADASWRFSYLSERAESVLGYARSELLGRALWELVPLGEERAMRDWLAGHGGEGRPF
                     RDLVHRMMTKAGGVTWQSLSGVPLQDAHGAWIGYRGTAADVTPRKQAEARIEQLTTRD
                     PLTGLANRTVLADRVSQAIVRAALARGELALLAIDLDRYRLVNESLGYPAGDALLRAV
                     AERLQNALRREDTLARLGGDDFALFWNGVRSADEAAALAGRIVAILGRPFTVEGQTLH
                     VEASVGIALYPRHGRDFGELLRNADAALYHAKSTGRGDFAFFEPGMHERAVEQLRTES
                     DLKGALARGELLLHWHPVVRGRGEVIGAEALVRWQHPARGFVSPESFVPFAEDSSLIR
                     ALGEWTLERAISQAGAWQRERRDAGGALWVAVNVSAAELA"
BASE COUNT          286 a          835 c          799 g          295 t
ORIGIN      
        1 ttcgcgagct ccgccgcgga gacgttgacc gcgacccaga gcgcgccgcc cgcgtcccgc
       61 cgctcgcgct gccatgcgcc ggcctgcgag atcgcgcgct cgagcgtcca ttccccgagc
      121 gcgcggatca ggctcgagtc ctcggcgaac ggcacgaagc tctccgggct cacgaacccg
      181 cgcgccgggt gctgccagcg caccagcgcc tcggcgccga tcacctcgcc gcgcccgcgc
      241 accaccggat gccaatgcag gaggagctcg ccgcgcgcga gcgcgccctt caggtcgctc
      301 tcggtgcgca gctgctcgac tgcccgctcg tgcatccccg gctcgaagaa cgcgaagtcg
      361 ccgcggccgg tgctcttcgc gtgatagagc gccgcgtcgg cgttgcgcag cagctcgccg
      421 aagtcgcggc cgtggcgcgg gtagagcgcg atgccgaccg atgcctcgac gtgcagcgtc
      481 tggccttcga cggtgaacgg ccggccgagg atcgcgacga tgcgccccgc cagcgcggcc
      541 gcttcgtccg ccgagcgcac gccgttccag aacagcgcga agtcgtcgcc gccgaggcgc
      601 gccagcgtgt cttcgcgccg cagcgcgttc tgcagccgct cggcgaccgc gcgcagcagc
      661 gcgtcccccg ccgggtagcc gagcgattcg ttcaccaggc gatagcggtc gaggtcgatc
      721 gcgaggagcg cgagctcgcc gcgcgcaagc gcggcgcgca cgattgcctg cgacacccgg
      781 tcggctagca ccgtccggtt ggctaggccc gtgagcggat cgcgcgtggt gagctgctcg
      841 atgcgcgcct ccgcctgctt gcgcggcgtg acgtccgccg cggtgccgcg gtagccgatc
      901 cacgcgccgt gcgcgtcctg cagcggcacg ccggagagcg actgccacgt gacgccgccc
      961 gccttcgtca tcatgcggtg caccaggtcg cggaagggcc gcccttcgcc gccgtgcccg
     1021 gcgagccagt cgcgcatcgc gcgctcctcg ccgagcggca cgagctccca gagcgcgcgg
     1081 ccgaggagct cgctccgcgc gtagccgagg acgctctcgg cgcgctcgga aaggtagctg
     1141 aagcgccagc tcgcgtcggc ttcccagacg tactcgcggg agacggacgc gacgtcgcgg
     1201 aaccgccggt cgcgctccgc cgccgccgcc tcggcgcgct tgcgcgcgtc gatgtcggcg
     1261 acgatgccgg acatgcgcag cgcccggcca tcggcgtcgc gctcggtcac gcgcccgcgg
     1321 cagtgcagcc agcgccagtc gccctcgcgg gtgcggatgc gcagctcgac gtcgtactcc
     1381 gcgtcgaggc ccttcagcgc gcggatcgtc gcctcgcgca acctcgccgc gtcgtccgca
     1441 tgcacgaggc cggcgagctg cgagccgcgt gtgatcggcg gcgagcggtc gtggccgagg
     1501 aacctcaccc agccgtcgcc ggcgtacagg tcgtccgact cgagatcgag gtgccattcg
     1561 gcgaggttgc cgccgtcgag cgccatctcc aggcgctcct tggcggcggc gaggccgcgc
     1621 tcggcgcggc gctgctcggt cacgtcgcgc ccgacgccgc ggtagccgac gaagcttccg
     1681 tgcgcgtcct tgcgcgactg cccggaaatg atgtgcacct ggcgcgcgcc gcgatcgtcg
     1741 gtgcgcgcga tctcgaggtc gaagaacgga gcctcggctt cgagccgctc gagcagcccg
     1801 tcgagcgcgc gccggtcgac ctccatgcgc gggatctccc agaagcgctt gccgtaggtc
     1861 ggccggccgc cgaacagcgt cgcgaccggg ccgccgcccg agagccaggt gatgcggtgc
     1921 tcggcgtcgg tctcccagaa ccagtcggcg gagagctcgg tcaggccgcg gacgcgcgcc
     1981 tcggcggcgc gcgcctgcgc gagcaggcgt tcaaggaagg catgaagccg ctgcgcatct
     2041 ccggggcgat gaaggtcgcc gcggggctga cgacgatcga ggaagtcctg aaggtcgcgc
     2101 cgccggtacg cgacggcacg cgctcctgac gtgatcgaag ccctggaatt cacggcgatc
     2161 tgctgcacga tcgtctatct cgtcatgcac ctgctgtcgc ggcggcgcga gcgcc
//
LOCUS       DATDMW010000079         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2263565, whole genome shotgun sequence.
ACCESSION   DATDMW010000079 DATDMW010000000
VERSION     DATDMW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2263565"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VI319_03415"
     CDS             <1..401
                     /locus_tag="VI319_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEY6862925.1"
                     /translation="PRPAFKFPGLWLYNGEPPADLEHSKGNYGIVHVMGVDPDDPQGL
                     IDTVGYVDPESLQAGTGSLDHIALSVTGRESMVARCKQAGLKYFERTVPTLGLHQMFL
                     KDPNGVMIELNFPASEAPKQRDETTTQAFR"
     gene            406..1611
                     /locus_tag="VI319_03420"
     CDS             406..1611
                     /locus_tag="VI319_03420"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6862926.1"
                     /translation="MAALPERRSGRVITYEQALASVRELAPKLKARAAVAESSRRIPE
                     ESLRELHASGLMRVLQPRRAGGSELPWVSLIDIGSGLAAACGSTAWNWVNYAVHHWML
                     AFWPVAVQDEIWSADPDMLIASSVVFPAGKATRVPGGYRLSGRWPFSSGVDQSLWNML
                     GGLVRDGEAPAEYRLFLLPRKDYSIIDNWHVMGLRGTGSNDVEAKDAFVPEERTLSID
                     ATRGGAKHPGAEVNPGAIFRIPVFATFPYMLTGVALGVAQGAYDDFLAGLRDRVARYS
                     GKSLADMTPVQIRIAEAAACIETARTVMRTHCQEAQDYAERDEVPDLAMKVRWRRDGA
                     FSAGLCERAVDVLFKCGGGGALFDGHPLQRAFRDVHAATAHISMIWEPQATTFGRVAL
                     GLPSDNPTL"
     gene            complement(1613..>2224)
                     /locus_tag="VI319_03425"
     CDS             complement(1613..>2224)
                     /locus_tag="VI319_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6862927.1"
                     /translation="NGKSTLMKCIMGIVVPKSGRITDTIYCVEHDLVGLRPEQIVDLG
                     VSLVPEGRRLFPRLDVEENLLLGAHRRAARAHLARKLEFCFATFPVLAQRRSQLAGSM
                     SGGEQQMLTLARALMTEPRILLIDEPSVGLAPLLVIHTIDKIRELKAARALTVLMAEQ
                     NFHQAIKIADRGYVIVHGRIAYEGASRDELSDNELVRKLYLGM"
BASE COUNT          346 a          733 c          769 g          376 t
ORIGIN      
        1 ggccgcggcc agcgttcaag ttccccggcc tgtggctcta caacggcgag ccgcccgcgg
       61 atctcgagca ctccaaaggc aactacggca tcgtccatgt catgggggtg gatcccgacg
      121 acccacaggg gctcatcgac acggttgggt acgtggatcc ggagtcgctc caagccggca
      181 ccggatcgct cgatcacatc gccctgtcgg tcaccggacg cgagagcatg gtcgcacgct
      241 gcaagcaagc gggtttgaag tatttcgagc gcaccgtccc caccctcgga ctgcatcaga
      301 tgttcctgaa ggatcccaac ggcgtgatga tcgagctcaa cttcccggcg tcggaagccc
      361 cgaagcagcg cgacgagacg acgacccagg ctttccggta acgccttggc tgcgctgccc
      421 gagcgccgtt ctgggcgcgt catcacgtac gaacaggcgc tggcgagcgt tcgcgagctc
      481 gccccgaagc tgaaggcgcg tgccgcggtc gccgagagct cgcgacgcat tcccgaggaa
      541 tcccttcgcg agcttcacgc ctcgggcttg atgcgtgtat tgcagccgcg ccgggccggg
      601 ggctccgagc tgccgtgggt gtcgctcatc gacatcggct ccggtctggc tgccgcttgc
      661 ggctcgaccg cctggaactg ggtcaactac gcggtgcatc actggatgct cgcgttctgg
      721 cccgtcgcgg tgcaggacga gatctggagc gcggacccgg acatgctcat cgcctcctcg
      781 gtcgtgtttc ccgcgggcaa ggcgacgcgc gtgcctggag gctatcggct ctcgggtcgg
      841 tggccgttct cgagcggcgt cgatcagagc ctgtggaaca tgctcggcgg cctcgtgcgg
      901 gacggcgagg cgcccgccga gtaccggctg ttcctgctgc cccgaaagga ctattcgatc
      961 atcgacaact ggcacgtgat gggcttgcgc ggcactggca gcaacgacgt cgaagcgaag
     1021 gacgcgttcg tgccggagga gcgcaccctt tctatcgatg cgacgcgcgg cggcgcgaaa
     1081 catccgggcg ccgaggtcaa cccgggcgcg atcttccgca tcccggtgtt cgccacgttt
     1141 ccgtacatgt tgacgggtgt cgccctcggc gttgcgcagg gtgcatacga cgattttctc
     1201 gccggcctgc gcgaccgcgt cgcgcgctac tcgggcaaga gcctcgccga catgacgccg
     1261 gtccagatcc gcatcgccga ggcggcggcg tgcatcgaga cggctcgcac ggtgatgcgc
     1321 acgcactgcc aggaagcgca ggactacgcc gagcgcgacg aagttcccga cctggcgatg
     1381 aaggtgcggt ggcgccgcga cggcgcgttc agcgcgggac tgtgcgagcg tgcggtagac
     1441 gtgctgttca aatgcggcgg tgggggcgcg ctcttcgacg gccatccgct gcaacgcgct
     1501 ttccgcgatg tgcacgccgc cacggcgcac atctcgatga tctgggagcc gcaggcgacg
     1561 acgttcgggc gcgtggcgct gggcttaccg agcgacaatc cgacgctgtg acctacattc
     1621 ccagatagag cttgcgcacc agctcgttgt cggacagctc gtcgcggctc gcgccctcgt
     1681 aggcgatgcg cccgtgcacg atgacgtagc cgcggtcggc gatcttgatc gcctggtgga
     1741 agttctgctc cgccatgagg acggtgagcg cccgcgccgc cttgagctcg cggatcttgt
     1801 cgatggtgtg aatgaccagc agcggcgcga gtccgaccga cggctcgtcg atcaggagga
     1861 tgcgcggctc ggtcatcagc gcccgggcga gcgtgagcat ctgctgctcg ccgccgctca
     1921 tgctgccggc gagctggctg cggcgctgcg cgagcacggg aaaggtcgcg aagcagaact
     1981 ccagcttgcg ggcgagatgc gcccgggcgg cgcggcggtg cgcgcccagg agcaggttct
     2041 cctcgacgtc gaggcgcggg aagagccggc gcccttccgg caccagcgag acgccgagat
     2101 cgacgatctg ttcgggccgc aagcccacga ggtcgtgctc gacgcagtat atcgtgtccg
     2161 tgatgcgtcc cgacttcggc acgacgatgc ccatgatgca cttcatgagc gtgctcttgc
     2221 cgtt
//
LOCUS       DATDMW010000080         3623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_769560, whole genome shotgun sequence.
ACCESSION   DATDMW010000080 DATDMW010000000
VERSION     DATDMW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769560"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VI319_03430"
     CDS             <1..555
                     /locus_tag="VI319_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY6862928.1"
                     /translation="VTVRGSRVRRARRIAESLEAAGIAGSFEGAMARAENPEIVSRTH
                     FARFLVERGHARDVRSVFHHYLVKGKPGFVPHEWAALGDAVSWIRTAGGSAVIAHPGR
                     YNLSRPEVRELIAEFMQHGGTGIEVVTGSHTPEQYAEFAALARELGLLASRGSDFHGP
                     GESKVELGSLPQLPADLRPVWRDW"
     gene            557..1192
                     /locus_tag="VI319_03435"
     CDS             557..1192
                     /locus_tag="VI319_03435"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY6862929.1"
                     /translation="MAQYFQIGIAHPQRRLISRAAQIVREGGVIAYPTDSCYALGCHI
                     GDKDAMLRLRRIRRFDERHHFSLVCRDLSEIGAYAKVDNAQYRLLKTLTPGSYTFILR
                     GTREVPRRLLHPKRKTLGVRVPDHPVAQALLAELGEPLLSTTAKLPGDDAPLDGGAQI
                     RQRLEHDVDLILDAGSCGTEPTTIIDLTGGEALVVRRGRGSLATLSIEAPL"
     gene            1247..1909
                     /locus_tag="VI319_03440"
     CDS             1247..1909
                     /locus_tag="VI319_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY6862930.1"
                     /translation="MELNLVQTIAIYALPLVLAITLHEAAHAYVAMHCGDRTAWMLGR
                     VSLNPVRHIDPTGTILLPLLLLAVSHGSFAFGWAKPVPVNFQALRNPKRDMFWVAAAG
                     PAANLVMALAWAAVLKVCDVSGMLDDQFLVQTAAAGIRINVLFMALNLLPVLPLDGGR
                     MLTSILPRRAALRFSKLEPWGLPVLLGVISLSYFGINVLGVLLAPIMNASMAIIGSLF
                     QL"
     gene            1914..3116
                     /locus_tag="VI319_03445"
                     /pseudo
     CDS             1914..3116
                     /locus_tag="VI319_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
     gene            3125..>3623
                     /locus_tag="VI319_03450"
     CDS             3125..>3623
                     /locus_tag="VI319_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY6862931.1"
                     /translation="MNDPVQLAIATPVAPATIARVRGQPFTELPHDLYIPPDALEVFL
                     ETFEGPLDLLLYLIRKANLDILDIPMARLTQQYIEYVEVMRTKNLELAAEYLVMAAML
                     MEIKSRMLLPKPPSAEGDEEDPRAELVRRLLEYERMKSAAQKLDELPHAGRDFIPVEV
                     WVEQAL"
BASE COUNT          585 a         1275 c         1204 g          559 t
ORIGIN      
        1 gtgaccgtgc gcggcagccg cgtgcgccgc gcgcgcagga tcgccgagtc gctcgaggcg
       61 gcgggcatcg cgggcagctt cgagggcgcg atggcgcgcg cggagaatcc ggagatcgtg
      121 agccgcactc atttcgcccg cttcctcgtg gagcgcggcc atgcgcgcga cgtgcgcagc
      181 gtgttccacc actacctggt caagggcaag ccgggcttcg ttccgcacga atgggcggcg
      241 ctcggcgacg ccgtgtcgtg gatccgtacg gcaggcggct cggccgtgat cgcgcatccc
      301 ggccggtaca atctctcccg ccccgaggtg cgcgagctga tcgctgaatt catgcagcac
      361 ggcggcacgg gcatcgaggt cgtcaccggc agccacacgc ccgagcagta tgcggaattc
      421 gccgctctcg cgcgcgagct ggggctgctc gcctcgcgcg ggtcggattt ccacgggccg
      481 ggcgagagca aggtcgagct cggatcgctg ccgcagctgc ccgccgacct gcggccggtg
      541 tggcgcgact ggtgaagtgg cccaatattt ccagatcggc atcgcgcacc cgcagcgccg
      601 gctgatctcg cgcgccgcgc agatcgtgcg cgagggcggg gtgatcgcgt atcccacgga
      661 ctcgtgctac gcgctcggct gccacatcgg cgacaaggac gcgatgctgc ggctgcggcg
      721 catccgccgc ttcgacgagc gccaccattt ctcgctcgtg tgccgcgatc tttccgagat
      781 cggcgcctac gccaaggtcg acaacgcgca gtaccggctg ctcaagacgc tgacgcccgg
      841 cagctacacc ttcatcctgc gcggcacgcg cgaggtgcca cggcgcctgc tgcacccgaa
      901 gcggaagacc ctcggcgtgc gcgtccccga ccacccggtt gcgcaggcgc tgctcgccga
      961 gctgggcgag ccgctgctgt ccacgaccgc aaagcttccc ggcgacgacg cgccgctcga
     1021 cggcggagcg cagatccggc agcgcctgga gcacgacgtc gatctcattc tcgacgcggg
     1081 atcgtgcggc accgagccga ccacgatcat cgacctcacc ggcggcgagg cgctggtcgt
     1141 gcgccgtggc aggggatcgc tcgcgacgct ctcgatcgag gccccgctct gataaaatcg
     1201 gccgcatgca aggccccaag aaaaatcggc acccgcccgg cggctaatgg agctcaacct
     1261 cgtccagacc atcgcgatct acgcgctgcc cctcgtcctc gcgatcacgc tgcacgaggc
     1321 cgcccacgcc tatgtcgcca tgcactgcgg cgaccggacc gcgtggatgc tgggacgggt
     1381 cagcctcaat ccggtgcgtc acatcgatcc gaccggtacg atcctgctgc cgctgctgct
     1441 gctcgcggtc tctcacggca gcttcgcgtt cgggtgggcg aagcccgtcc cggtcaattt
     1501 ccaggcgctg cgcaatccga agcgcgatat gttctgggtc gccgccgccg gccccgccgc
     1561 gaacctggtc atggccctcg cgtgggccgc ggtgctgaag gtgtgcgacg tgtcgggcat
     1621 gctggacgat cagttcctcg tgcagacggc ggccgccggg atccgaatca acgttctctt
     1681 catggcgctg aatctgctcc cggttcttcc gctcgacggc ggtcggatgc tgacttcgat
     1741 tctgccgcgc cgcgccgccc tgcgtttctc gaagctcgag ccctggggac tcccggtgct
     1801 gctcggcgtg atctccctca gctacttcgg gatcaacgtg ctcggcgtgc tgctggcccc
     1861 gatcatgaat gcctcgatgg cgatcatcgg gtcgctgttc cagctctgac gccatgttcg
     1921 aggaacgcgt cgtctccgga atgcgcccca ccgggcgcct gcacctcggc cactggcacg
     1981 gtgcgctcaa gaactgggtg cggctgcaag ccgagcgccc gtgcctgttc ttcgtcgccg
     2041 actggcatgc gctcaccacg cactacgaga ctcccgaggt gatcgaggag agcgtctggg
     2101 aaatggtgat cgactggctc gccgccggca tcgacccggc gcaggcgacc ctcttcatcc
     2161 agtcgcgcgt cgtcgagcac gccgagctgc acacgctgct ctcgatgatc acgccgctcg
     2221 gctgactcga gcgcgtgccg acctacaagg agcagcagga gaagatcgcc gaccgcgacc
     2281 tcgcgaccta cggctttctc ggctacccgt tgcttcaggc cgccgacgtg ctcttgtatc
     2341 gaggtaaata cgtcccggtc ggcgaggacc aggttccgca catcgagctg atgcgcgagg
     2401 tggcgcgccg cttcaaccat atctatggcc gcgagcccgg tttcgaggac aaggcgaagg
     2461 ccgccgccaa gaagatgggc gcgcgcaagg ccaagctcta cgcgcagctg cgcaccaagt
     2521 accaggagca gggcgacgcc gacgcgctcg ccgcggcgcg cgcgctgatc gacgagcagg
     2581 cgaacctcgc gctcggcgac aaggagcgcc tgttcggcta catcgagggc ggcgggcgca
     2641 tgatcctggt cgagcccgac gcgatgctca ccgaggcttc gcgactcccg ggcctcgacg
     2701 gccagaagat gtcgaagtcg tacggcaata ccatcgagct gcgcgaggac ccggaatcgg
     2761 tgaggaagaa gatccgcacc atgcagaccg atccggcgcg cgtgaagcgc agcgacccgg
     2821 gcgatcccga caagtgcccg gtgtggcagt tccacctcgt ctacagcgac gacaagacga
     2881 gaacctgggc gaaccagggc tgccgctccg ccggcatcgg ctgcctcgag tgcaagcaac
     2941 cggtgatcga ctcggtgctc gccgagctcg cgcccatgcg cgagcgcgcg aagcgctacg
     3001 tcgacgaccc gaccctggtg cgcaacatcg tcgccgacgg caacgagaag gcgaagcgtc
     3061 tcgccgagga gacgatgcgc gacgtgcgcc cggcgatggg tctcgcctat acgtgaatga
     3121 gcacgtgaac gatcccgtcc agctcgccat cgccacgccg gtcgcgccgg ctaccatcgc
     3181 ccgggttcgc gggcagccct tcaccgagct gccgcacgac ctctacatcc cgcccgacgc
     3241 gctcgaggtc ttcctcgaga ccttcgaggg gccgctcgac ctgctgctct acctgatccg
     3301 caaggcgaac ctcgacatcc tcgacatccc gatggcgcgg ctcacccagc agtacatcga
     3361 gtacgtcgag gtgatgcgca cgaagaacct cgagctcgcc gcagagtacc tggtcatggc
     3421 ggcgatgctg atggagatca agtcgcgcat gctgctgccc aagccgccgt ccgccgaggg
     3481 cgacgaggag gacccgcgcg ccgagctggt gcggcggctg ctcgagtacg agcgcatgaa
     3541 gagcgccgcg cagaagctcg acgagctgcc gcacgcggga cgggacttca tcccggtcga
     3601 ggtctgggtc gagcaggcgc tcg
//
LOCUS       DATDMW010000081         7611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1267520, whole genome shotgun sequence.
ACCESSION   DATDMW010000081 DATDMW010000000
VERSION     DATDMW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267520"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VI319_03455"
     CDS             <1..228
                     /locus_tag="VI319_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6862932.1"
                     /translation="MKDRWVQAEVNDLIERRLSPNPLVTIHKYPEADHAFARHGSRVY
                     RKDDADRALALSLGFFRKYLGQLSRDPSRSA"
     gene            complement(200..913)
                     /locus_tag="VI319_03460"
     CDS             complement(200..913)
                     /locus_tag="VI319_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY6862933.1"
                     /translation="MADAAALKLLSADVLTPVLTALVREFERTTQRRVTLALATTGVV
                     RDRVRAGETADVAIMQRPSMAMLAFDGRIVGDTIVDLGRSSVGVGVRAGAPRPDLGSV
                     EGFKRSLLAAKSIGYTDPRKGGGSGAYFAALLEKLGLAGRLQPKTRYPGPGHSAADLI
                     AEGGIDFGIAQPMEILARTGIELAGVLPAELQDPELFVFSAGVLKRSQEPAAAKSLIA
                     FLSGPAARAAIRRNGMDPG"
     gene            complement(924..1895)
                     /locus_tag="VI319_03465"
     CDS             complement(924..1895)
                     /locus_tag="VI319_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6862934.1"
                     /translation="MNLRLVLLALAFVPGFTGGAHAQDYPSRAITFVAAGPAGGPTDA
                     IGRIVGERMRASLGQPVLIENVSASGGAAVRRVGRAAPDGYTIELGHWGTNVVDGAAL
                     ALPFDLLRDFEPVSLLTTNALVILSGNAVPAKNLKELIGWLKANPGKATLGSPGAGSP
                     PHIAGVFLQKLTDTRFVFVPYRGAAPAMQDLLAGHIDLSIPQAAAALPQVRAGRVRAY
                     AVTAKARLASAPDIPTVDEAGVPGLYISVWHGLWAPKGTPKGVIDRISKAAADALADP
                     AVRQRLADLGQEIPAADQQTPEGLRRLQTAEIEKWWPIVKAADIRSE"
     gene            2019..2951
                     /locus_tag="VI319_03470"
     CDS             2019..2951
                     /locus_tag="VI319_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6862935.1"
                     /translation="MKLSQLRTFVAVVDGGTVARAALRVRLTQSAASRQIASLEDELG
                     VALFDRIGRRVQLTAEGDDLLKRARRLLADAEALGERARVLKGGQSGTLRVSATPQVI
                     ETMLAPFLPRYLRASPEVEVQLLESGGPQQIEELDRGEAQLALMAYGVERFDSRLLYP
                     IHLLAVVSARHRLKSAATCDLAKLADEPLMILGREFGARSWFDGACENARVSPRVVLE
                     SSSPHTLLALAGVGHAIAVVPSNVGSLPPSVRAVPLVHQGRAIGRWSMIAWRPERFLA
                     PYARRFVDELVDYAQRAYPGRGLSRRAPPLPRPH"
     gene            complement(2965..3945)
                     /locus_tag="VI319_03475"
     CDS             complement(2965..3945)
                     /locus_tag="VI319_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6862936.1"
                     /translation="MNQNRRRVLALGLAALAARPSFAQSKYPERPIRLVIPFPPGGVN
                     DAVGRPWAEKMKPLLGTVVVENQGGAGGVIGATAVAHAQPDGYTLLLGGGSSHVINPI
                     AASRPTYDPMKDFEAIAILAVSGLGIVVNPALPVRTLKDLIAYARANAAKMSYASAGV
                     GTVTHIGAELFKSLTGTGAMVHVPYKGAGPAIADVMGGQVPLAMPNLTGQVLKLHEAG
                     KLRLLAVTTPRRLAAAPDIPTAVEAGLPGMIAQNFTALFAPAHTPAAIVEQIARATRA
                     AMSDPEFRRQLVASGFEPETDSSPEKARAFVREEIARWTPVVKSIALKLD"
     gene            4058..5035
                     /locus_tag="VI319_03480"
     CDS             4058..5035
                     /locus_tag="VI319_03480"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6862937.1"
                     /translation="MNLNLKGLLAVRAVIADGTVTAAARRLHRTQPVVSRLIAQVEAQ
                     VGFALFRRDGRRLLPTPQGLAFYRETERALSALAEIESAARGIGARRDAPLRILAQSH
                     MAHGLLHVVLGEFCGRHPDFRFAVEIRQREFISHWIANRQFDVGFAPRPVDHPQVESE
                     LFVRAPLFVAMPAGHRLAPKRDVRPEDLADEPLVAGRPGSPLRASLDALFARLGAAPQ
                     IRGETFTAVSACQLAGKSVGVALADPFVPSMFLDDPSIAFRPLRGHGAHDYLVLWPAG
                     QERSALAGEFIDCARKRARLVIDAVLRRASPQRKALNYTRSSARSKGAP"
     gene            5032..6006
                     /locus_tag="VI319_03485"
     CDS             5032..6006
                     /locus_tag="VI319_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862938.1"
                     /translation="MIRILAAVLAFAAVPALAQTKLDMVGFGGASNLPVWVAQERGLF
                     AREGLDVKFDRTPSSGALFRNLMAGKYQIASAAFDNVVAYVEGQAEQTIEGFDVVSFL
                     GVHGGLVSLMTTPDVEGFAGLKGKAAAVDSAASGYALLMYRMLDQKGLAKDRDYRVVS
                     VGGTGERLQAMREGRAAAGMISPPQDLEAKKLGFRTLGEPTKALGAYAGSVYNVRRAW
                     AKDHEKELVAFARAIVAAHDLIFTDRAAAIAVLRKRLKELGPEDAAAVYAKMTGPGGF
                     TRKAQISIRGARVVLSVRSQYGEPRKKLTDPYKYMDLRYYRKAMQSGG"
     gene            6071..6307
                     /locus_tag="VI319_03490"
     CDS             6071..6307
                     /locus_tag="VI319_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862939.1"
                     /translation="MSSFVRGGFCPNCNGLLYLLSRDRETEPWQHSGASVREDREGDH
                     IRCAVCAYRVAVRRSDATPGWGYEIDLPPRGRGN"
     gene            6399..6599
                     /locus_tag="VI319_03495"
     CDS             6399..6599
                     /locus_tag="VI319_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862940.1"
                     /translation="MGIDEERQTALASEPQDKLLAEERPDFHGSQEPSRTERYFVDFR
                     DRNGNDMVDLLLMLHCCSPHAA"
     gene            complement(6639..6965)
                     /locus_tag="VI319_03500"
     CDS             complement(6639..6965)
                     /locus_tag="VI319_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862941.1"
                     /translation="MKEIMSLNRDDRIWQQLHEQPEPVGGRYAPVAGTFVRCAGCSRD
                     FRSTLTFKTLHDFERAATVGFVAQCPNCYAIFECNRRNMYCELAARDHSAVSEVVPMA
                     ARRRLE"
     gene            7143..7373
                     /locus_tag="VI319_03505"
     CDS             7143..7373
                     /locus_tag="VI319_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862942.1"
                     /translation="MPIYLLIPLETGHPAWRGITRRSPIQVLAENEREARAAAWLRYG
                     SASKSEDDPWLLGKWVYAHVIDAPDPHLPMLE"
     gene            complement(7412..>7611)
                     /locus_tag="VI319_03510"
     CDS             complement(7412..>7611)
                     /locus_tag="VI319_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862943.1"
                     /translation="QRAVFRCPLCGSRTFAAVEITLRNGARFTADFYHCTECSLHFVH
                     PDRFMRAIDGAAAIEPSAAAA"
BASE COUNT         1157 a         2666 c         2639 g         1149 t
ORIGIN      
        1 atgaaggacc gctgggtgca ggccgaggtg aacgacctga tcgagcggcg gctctcgccc
       61 aatccgctcg tcacgatcca caagtatccc gaggccgacc acgccttcgc gcgccacggg
      121 agcagggtgt accgcaagga cgacgccgac cgggcgctcg cgctgtcgct cgggttcttc
      181 cggaaatatc tcggccagct cagccgggat ccatcccgtt ccgcctgatc gcggcccgcg
      241 cggccggacc cgacaggaac gcgatcagcg atttcgccgc cgccggctcc tgcgagcgct
      301 tcagcacccc ggcggaaaaa acgaaaagct cgggatcctg caattccgcc ggcagcacgc
      361 ccgcgagctc gatcccggtc ctggcgagaa tttccatggg ctgcgcgatg ccgaaatcga
      421 tcccgccttc ggcgatgaga tcggcggcgg aatggccggg gcccggatac cgggttttcg
      481 gctgcagccg tccggcgagg ccgagcttct cgagcagcgc cgcgaagtag gccccgctgc
      541 cgccgccctt gcgcggatcg gtatagccga tcgatttcgc cgcgagcagc gagcgcttga
      601 atccctccac ggagccgagg tcgggcctcg gcgcgccggc gcgcacgccg acgccgacgg
      661 aagagcgtcc caggtcgacg atcgtgtcgc cgacgatcct gccgtcgaaa gcgagcatgg
      721 ccatggaagg ccgctgcatg atggcgacgt ccgcggtctc tccggcccgg acgcggtccc
      781 tgaccacgcc cgtcgtggcg agcgccagcg tcacgcgacg ctgcgtcgtg cgctcgaact
      841 cgcgcacgag agccgtcagc accggcgtca gcacgtcggc gctgagcagc ttcagcgccg
      901 cggcatccgc catgggcgcc tgctcactcg gacctgatgt ccgcggcctt gacgatcggc
      961 caccatttct cgatctccgc cgtctgcagc cgccgcagcc cttccggcgt ctgttgatcc
     1021 gccgccggta tctcctgtcc gagatcggcg agccgttgac gcacggccgg gtccgccagg
     1081 gcgtcggccg ccgccttgct gatcctgtcg atcacgccct tgggcgtgcc tttgggcgcc
     1141 cagagcccgt gccagacgga aatatacagg cccggcaccc cggcctcgtc gacggtcggg
     1201 atatcgggcg ccgacgccag ccgtgccttc gcggtgacgg cgtaagccct cacgcgtccg
     1261 gcgcggacct gcggcagggc ggccgccgcc tgcggaatgc tcaggtcgat gtgcccggcg
     1321 agcagatcct gcatcgcggg cgcggcgccg cgatacggca cgaagacgaa gcgcgtgtcg
     1381 gtcagcttct ggagaaagac gccggcgata tgcggggggc tcccggcgcc cgggctgccg
     1441 agcgtcgcct tccccgggtt cgccttcagc cagccgatca gctccttcag gttcttcgcc
     1501 ggaacggcgt tcccggagag gatgacgagc gcgttggtgg tcagcaacga gaccggctcg
     1561 aagtcgcgca gaagatcgaa cggaagcgcg agcgcggcgc cgtcgaccac gttcgtgccc
     1621 caatgcccga gctcgatcgt gtagccgtcg ggagccgcgc gcccgacgcg gcggacggcc
     1681 gcgccgcccg aggccgagac gttctcgatg aggaccggct ggccgagcga ggcgcgcatg
     1741 cgctcgccga cgatgcggcc gatggcgtcg gtcggcccgc cggccgggcc ggcggcgacg
     1801 aaggtgatcg cgcgcgacgg gtagtcctgc gcgtgcgcgc cgcccgtaaa accggggacg
     1861 aaagccagtg ccagcaagac gagacgaagg ttcatggcgc tcccagggtc caatcccggc
     1921 gggccatccg ccaggcgggg cgcaaattag cacgcgcgca ttgatgcgtc caattcattt
     1981 gaagtatgat ttatcatgat tttatggaat aagcgccgat gaagctgagc cagctgcgca
     2041 ccttcgtcgc cgtcgtcgac ggcggcacgg tggcccgtgc cgccctgcgg gtgcggctca
     2101 cccagtcggc cgcatcgcga cagatcgcct cgctggagga cgagctgggc gtggcgcttt
     2161 tcgatcgaat cgggcgccgc gtccagttga ccgcggaggg cgacgacctg ctgaagcgcg
     2221 cccgcaggct gctcgcggac gccgaggcgc tcggagagcg cgccagggtc ctcaagggcg
     2281 ggcaatcggg cacgctgcgc gtcagcgcga cgccgcaggt gatcgagacg atgctcgccc
     2341 ccttcctgcc gcgctacctg cgcgcgagcc cggaagtcga ggtccagctc ctcgagagcg
     2401 gcggcccgca gcagatcgag gagctcgatc gcggcgaggc gcagctggcg ctcatggcgt
     2461 acggggtcga gcgcttcgac agccggctcc tgtatccgat ccatctgctg gccgtggtct
     2521 cggcgcggca tcggttgaag agcgccgcca cgtgcgacct cgcgaagctc gccgacgagc
     2581 ccctgatgat cctgggccgg gaattcggcg cgcgctcctg gttcgacggc gcctgcgaga
     2641 acgcgcgcgt gagcccgcgg gtcgtgctcg aaagcagctc tccgcatacg ctgctcgcgc
     2701 tggcgggagt cgggcacgcg atcgccgtcg ttccaagcaa cgtcggcagc ctgccgccct
     2761 cggtgcgcgc ggtgccgctg gtccaccagg ggcgggccat cggacggtgg tcgatgatcg
     2821 cgtggcgccc ggaacggttt ctcgcgcctt acgcgcggcg gttcgtcgac gagctcgtcg
     2881 actatgcgca acgggcgtat cccggtcgcg ggctctcccg gcgggcgccg ccgctgccgc
     2941 gcccgcactg attccggctc gccgctagtc gagcttcagc gcgatcgact tcaccaccgg
     3001 cgtccagcgc gcgatctcct cgcgcacgaa ggcgcgcgcc ttctccggcg aggaatcggt
     3061 ctcgggctcg aagcccgagg cgaccagctg gcggcggaat tccggatcgc tcatcgccgc
     3121 gcgcgtcgcg cgcgcgatct gctcgacgat agcggcgggc gtgtgcgcgg gcgcgaacag
     3181 cgcggtgaag ttctgcgcga tcatcccggg gaggcccgcc tcgaccgccg tcggaatgtc
     3241 cggcgccgcg gcgaggcggc gcggcgtggt caccgcgagc aggcgcagct tacccgcctc
     3301 gtgcagcttc agcacctgcc cggtcaggtt gggcatcgcc agcggcacct gcccgcccat
     3361 cacgtcggcg atcgccgggc ccgcgccctt gtacgggacg tggaccatcg ccccggtgcc
     3421 ggtcagcgac ttgaagagct ccgcgccgat gtgggtcacc gtcccgacgc ccgccgacgc
     3481 atacgacatc ttcgcagcgt tcgccctggc gtaggcgatc agatccttga gcgtgcgcac
     3541 ggggagcgcg ggattgacca cgatgccgag ccccgacacc gccaggatcg cgatcgcctc
     3601 gaagtccttc atcggatcgt aggtcggccg cgacgcggcg atcgggttga tcacgtgcga
     3661 gctgccgccg ccgaggagca gggtgtagcc gtcgggctgc gcgtgcgcga ccgcggtggc
     3721 gccgatcacg ccgcccgcgc cgccctggtt ctcgaccacc acggtgccga gcagcggctt
     3781 catcttctcc gcccacggtc ggccgaccgc gtcgttgacg ccgcccggcg ggaacgggat
     3841 gacgaggcgg atggggcgct cgggatactt cgactgcgcg aacgaggggc gggccgcgag
     3901 cgccgcaagc ccgagcgcca ggacgcggcg ccgattctgg ttcatgttct cgtctcctcc
     3961 acggctcgag gccgccattg tcgccgcccg cgccttatgc ggtccaattc aaacttgtcc
     4021 tatgatcatg caataacaac ataagaaagg ccggcgcgtg aacctcaacc tcaagggcct
     4081 gctcgccgtg cgggcggtga tcgccgacgg caccgtcacc gccgcggcgc gacgtctgca
     4141 ccgcacccag cccgtcgtca gccggctgat cgcgcaggtc gaggcgcagg tcggattcgc
     4201 gctcttccgg cgcgacggcc ggcggctgct gccgacgccg cagggactgg cgttctaccg
     4261 cgagaccgag cgcgcgctgt ccgcgctcgc cgagatcgag tcggcggcgc gcggcatcgg
     4321 cgcgcggcgc gatgcgcccc tgcgcatcct ggcgcaatcg cacatggcgc acgggctgct
     4381 gcacgtcgtg ctcggcgagt tctgcggccg ccatccggat ttccgcttcg cggtcgagat
     4441 ccgccagcgc gagttcatca gccactggat cgcgaaccgc cagttcgacg tcggcttcgc
     4501 gccgcggccc gtcgatcacc cgcaggtcga gtccgagctg ttcgtgcgcg cgccgctgtt
     4561 cgtcgcgatg ccggcgggtc atcgtctcgc gcccaagcgc gacgtgcgcc ccgaggacct
     4621 cgccgacgag ccgctcgtcg ccggccggcc cggctcgccg ctgcgcgcga gcctcgatgc
     4681 gctgttcgcc cggctcggcg ccgcgccgca gatccggggc gagaccttca ccgccgtgtc
     4741 ggcgtgccag ctcgcgggga agtcggtcgg cgtggcgctc gccgacccgt tcgtccccag
     4801 catgttcctc gacgacccgt cgatcgcgtt ccggccgctg cgcggccacg gcgcgcacga
     4861 ctacctggtg ctgtggcccg cgggacagga gcgcagcgcg ctcgccgggg agttcatcga
     4921 ttgcgcgcgc aagcgcgcca ggctcgtgat cgacgccgtg ctgcggcgcg cctcgccgca
     4981 gcgcaaggcg ctgaactata ctcgctcgtc cgcacgctcg aaaggcgccc catgatccgg
     5041 attctcgccg ccgtcctcgc attcgccgca gtccccgcgc tcgcgcagac caagctcgac
     5101 atggtcggct tcggcggcgc gagcaacctg ccggtatggg tcgcgcagga gcgcggcctg
     5161 ttcgcaagag aagggctgga cgtgaagttc gaccgcacgc ccagctcggg cgcgctcttc
     5221 cgcaacctga tggcgggcaa gtaccagatc gcgagcgcgg ccttcgacaa cgtcgtcgcc
     5281 tacgtcgagg gccaggccga gcagacgatc gagggcttcg acgtcgtctc cttcctcggc
     5341 gtgcacggcg ggctcgtgtc cctgatgacg acgccggacg tcgagggctt cgccggcctg
     5401 aagggcaagg ccgccgcggt cgactccgcc gcgagcggct acgcgctgct catgtaccgc
     5461 atgctcgacc agaaggggct cgccaaagac cgcgactacc gcgtcgtgtc ggtcggcggc
     5521 acaggcgagc gtctccaggc gatgcgcgaa ggccgcgcgg cggcgggaat gatcagcccg
     5581 ccgcaggacc tggaggcgaa aaagctcggc ttcaggacgc tgggcgagcc gaccaaggcg
     5641 ctcggcgcct acgccggcag cgtctacaac gtgcgccgcg cgtgggcgaa ggaccacgag
     5701 aaggagctgg tcgcgttcgc gcgcgcgatc gtcgccgcgc acgacctcat cttcaccgac
     5761 cgggccgcgg cgatcgcggt gctgaggaag cgcctcaagg agctcggccc cgaggacgcc
     5821 gccgcggtct acgcgaagat gaccggcccc ggcggcttca cgcgcaaggc gcagatcagc
     5881 atccgcggcg cgcgcgtcgt gctctcggtg cgcagccagt acggggagcc gaggaagaag
     5941 ctcaccgacc cgtacaagta catggacctc cggtactacc gcaaggcgat gcagagcggc
     6001 ggctgatcgc ggccgccagt agtcagaagc gcggattgat ctttgcgcga ggccgctcta
     6061 gctttgacgc gtgtcgagct tcgtccgagg gggcttctgc ccgaactgca acggcctgct
     6121 ctatctcctt tcgcgcgacc gggagaccga gccctggcag cactcgggcg cctcggtgcg
     6181 cgaggaccgc gaaggcgatc acatccgctg cgcggtatgc gcctaccgcg tcgcggtcag
     6241 gcgcagcgac gcgactccgg ggtggggcta cgagatcgac ctgccgccga ggggccgcgg
     6301 gaactgagcg ccggacggag gacctatcga gcgcgatcgc tgggcgaatc cgcgccggac
     6361 agcatgtccg acccctcggg ccagggctcg ggacggggat gggcatcgac gaggagcgcc
     6421 agacggctct cgcttctgag ccgcaagaca aactcctcgc cgaggagcgt cctgattttc
     6481 atggctctca ggaaccgtcc cggaccgaac ggtatttcgt cgacttccgt gaccgcaacg
     6541 gaaacgacat ggtcgatctg ctgctcatgc tgcactgctg ctctccccac gccgcgtagt
     6601 tttgcggcgg acgttcttgt tattgcgctc ccctaacgct attccagccg cctgcgcgcc
     6661 gccatgggaa cgacctcgct cacggcgctg tgatcgcgcg ccgccaactc gcagtacatg
     6721 tttctcctgt tgcactcgaa aatggcatag cagttcgggc attgcgcgac gaagccgacg
     6781 gtcgccgcgc gctcgaagtc gtgcagggtc ttgaaggtca gggtggagcg gaaatcgcgg
     6841 ctgcagcctg cacagcgcac gaaggtcccg gcgacggggg cgtaacgccc gcccacaggt
     6901 tcgggctgct cgtgtagctg ctgccagatc cgatcgtcac ggttcaacga catgatttcc
     6961 ttcacgaatt cctggcgtta gagaggcgag cgccaaaatg gttcaatcgg agcagggacg
     7021 cgtgcgcgaa atcgacatgt gtcgtgttaa ttcgatgttt atgcgggttt tctcacgctc
     7081 ctcccggaac catcgggccg caggcggcaa ccaacgcgta cgcaacttcg gaggggagtt
     7141 acgtgcccat ctacctgctc attccgctgg aaaccggcca cccggcgtgg cgcgggatca
     7201 cccggcgcag cccgatccaa gtcctcgccg agaacgagcg cgaagcccgc gccgccgcgt
     7261 ggcttcgcta tggctcggcg tcgaaatcgg aggacgatcc ttggctgctc gggaaatggg
     7321 tgtacgcgca cgtcatcgac gccccggatc cgcaccttcc gatgctcgag tgaatggcgt
     7381 gcgcgtcgga ggcgcgcagg cgccgtagct gctacgcggc cgcggcagag ggctcgatcg
     7441 ccgcggcgcc gtcgatggcg cgcatgaagc ggtccggatg cacgaaatgc agcgagcact
     7501 cggtgcaatg atagaaatcc gcggtgaacc gcgcgccgtt gcgcagcgtt atttcgacgg
     7561 cggcaaaagt acgcgacccg cagagcgggc atctgaaaac cgcgcgttga a
//
LOCUS       DATDMW010000082         4922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1393065, whole genome shotgun sequence.
ACCESSION   DATDMW010000082 DATDMW010000000
VERSION     DATDMW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393065"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VI319_03515"
     CDS             complement(<1..223)
                     /locus_tag="VI319_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862944.1"
                     /translation="MSHDTDRSRRRALVALLGAGGGLAGALRLALAQTSAEGMRQVRG
                     EVTVNGKPVRVGDTVRPGDTVATGARSFAA"
     gene            371..2422
                     /locus_tag="VI319_03520"
     CDS             371..2422
                     /locus_tag="VI319_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY6862945.1"
                     /translation="MPNRLAQQTSPYLQQHADNPVDWYPWGDEALERARREDKPILLS
                     VGYSACHWCHVMAHESFEDPRVAEVMNRLFVNVKVDREERPDLDQIYQLAHQMMAQRP
                     GGWPLTMFLAPDGAPFFGGTYFPKEARYNLPGFPDLLRRVSEFYRQNREEIGRQNEGL
                     LAAFERLQPGAPAHHSELSGEPIDAALRALKTSFDSRWGGFGGAPKFPHAAELAFCLR
                     RHAATGDAHALDIAAVTLERMAQGGIWDHLGGGFCRYSVDAQWTIPHFEKMLYDNGPL
                     LALYADAWTVTRDPRFARVADGIAGWVMREMQSPEGGYYSSLDADSEHEEGKFYVWTP
                     DEARAHLTELEYAVLAPHYGLDGAPNFEGRHWHLRETAPLADVAARLGKPLDECARLL
                     DSARAKLFAERERRVHPGRDDKVLASWNALMIAGMARAALVFDRRDWLDSAKRAFAFL
                     RSTLWRDGRLLATWKDGRAHLNAYLDDYAYGVAAAIELLQAEYDADTLAFAEELAEVL
                     IEQFEDTVNGGFFFTSHDHERLIHRPKPGDDNATPSGNGMAALGLQRLSLLTGETRYA
                     ASAERAIAQFYPELAAQPGGHTTLLMALEERLVPPRTVILRGPAGPLVRWRRALAERH
                     LPATLVLALAPDARDLPPVLAKPASDRVNAWVCEGVTCLPPVDRLEDLIPQVSKAVEI
                     H"
     gene            2451..2765
                     /locus_tag="VI319_03525"
     CDS             2451..2765
                     /locus_tag="VI319_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6862946.1"
                     /translation="MNKKLVWFAAAGALVCAPALAQDAQQLLKDKACLACHSLDNKVV
                     GPAYKDVAKKFRARKDAESYLAKKIREGSTGEWGQIPMPPNTTVSEAEAHTLAKYILS
                     LK"
     gene            2776..3978
                     /locus_tag="VI319_03530"
     CDS             2776..3978
                     /locus_tag="VI319_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY6862947.1"
                     /translation="MAAVRVGSVLILACAIAGLAACGDDKKAAGAKGGATVTIGIAAP
                     LTGPQSHIGKDLENAVRLAADDANAAKVVVGGKPVHFEVQAEDDQADPKMAPVVAQKL
                     ADAHVAAVVGHFNSGTTIPASRIYNDAGIPQISPSATNPKYTEQGFKTAFRVVANDNQ
                     QGKVVGEYIAKDLKSKALAIIDDRTAYGQGVADVVERSAKAAGAVVVAREFTTDRSTD
                     FKAILTKIKGTRPDVIFFGGIDTQAGPMLQQMRGLGIEAQFVGADGIQSTELFKLGGN
                     AVEGTLASFPGLPLDRMPQGAGFGEKYRKKWNQDVVLYAPQGYDAFNIFVEAMKRAGS
                     TDPAKFLPEVAKTDYNGITGPIRFDEKGDLEDGPITIFKAVGGKWQAVATIGGPSQGQ
                     AAKPAEKN"
     gene            4079..>4922
                     /locus_tag="VI319_03535"
     CDS             4079..>4922
                     /locus_tag="VI319_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6862948.1"
                     /translation="MDILLQQLINGVTLGSVYALVALGYTMVYGIIGLINFAHGELVM
                     VGALTALSVIAALLRLGLGLPPAAMMLIALLAALPVCMLLAYAMERVAYRPLRRAPRL
                     APLITAIGVSIVLQNVAMLVWGRSYFSFPPILNVTNYDIAGATITDLQIVIVLLSAAL
                     MAGLLLIVHRTRLGLAMRATAENLGVAALMGVDANRIIVAAFLLGSALAAVAGLMVSA
                     YYGIAHYYMGFLLGLKAFTAAVLGGIGNIGGAMLGGLLVGLAETFTSGYLTSTFQDLV
                     VFSVL"
BASE COUNT          757 a         1749 c         1691 g          725 t
ORIGIN      
        1 aggcggcgaa agagcgcgcg ccggtcgcca ccgtgtcgcc cgggcgcacc gtgtcgccga
       61 cgcgcaccgg cttgccgttg accgtcactt cgccgcggac ctggcgcatg ccttcggcgc
      121 tcgtctgcgc gagcgcgaga cgcagcgcgc ctgcgagccc gccgcccgct ccgagcagcg
      181 cgacgagcgc ccggcggcgg ctgcgatcgg tgtcgtggct catcattcct cccccggttt
      241 cccgcgacgg cgacgccgca agtctattat gaggaacccg cgtcgggcga ccggagtcga
      301 acgctcctgc gcggaggaag tcacgccgtc cccgcgtgct acactcgact cccctccggc
      361 aaccgccgcc atgccgaacc gcctcgcgca acagaccagc ccctacctgc agcagcacgc
      421 ggacaacccg gtcgactggt acccgtgggg cgacgaggcg ctcgagcgcg cccgccgcga
      481 ggacaagccg atcctgctct cggtgggcta ctcggcgtgc cactggtgcc acgtgatggc
      541 gcacgagtcc ttcgaggacc cgcgcgtcgc cgaggtgatg aaccgactgt tcgtgaacgt
      601 caaggtcgac cgcgaggagc gccccgacct cgaccagatc taccagctcg cgcaccagat
      661 gatggcgcag cgccccggcg gctggccgct cacgatgttc ctcgcgcccg acggcgcgcc
      721 cttcttcggc ggcacgtact ttccgaagga ggcgcgctac aacctccccg ggtttcccga
      781 cctgctccgg cgagtctccg agttctaccg ccagaaccgc gaggagatcg gccggcagaa
      841 cgaggggctg ctcgccgcgt tcgagcggct gcagccgggc gcgcccgcgc accactccga
      901 gctctcgggc gagccgatcg acgccgcgct gcgcgcgctc aagacgagct tcgactcgcg
      961 ctggggcggc ttcggcggcg cgccgaagtt cccgcacgcc gcggagctcg cgttctgcct
     1021 gcgccgccac gcggcgacgg gcgacgcgca cgcgctcgac atcgccgcgg tcacgctcga
     1081 gcgcatggcg cagggcggta tctgggacca tctcggcggc ggcttctgcc ggtacagcgt
     1141 cgacgcgcag tggaccatcc cgcacttcga gaagatgctc tacgacaacg ggccgctgct
     1201 cgcgctctac gccgacgcgt ggacggtgac gcgcgacccg cgcttcgcgc gcgtcgccga
     1261 cgggatcgcg ggctgggtca tgcgcgagat gcagtcgccg gaaggcggct actactcctc
     1321 gctcgacgcc gactcggagc acgaagaggg caagttctac gtctggacgc ccgacgaggc
     1381 gcgtgcgcac ctgaccgagc tcgagtacgc cgtgctcgcg ccgcactacg gcctcgacgg
     1441 cgcgcccaac ttcgagggca ggcactggca cctgcgcgag acggcgccgc tcgccgacgt
     1501 cgccgcgcgg ctcggtaagc cgctcgacga atgcgcgcgg ctgctcgact cggcgcgcgc
     1561 caagctcttt gccgagcgcg agcgccgtgt ccaccccggg cgcgacgaca aggtgcttgc
     1621 cagctggaac gcgctcatga tcgccggaat ggcgcgcgcc gcattggtgt tcgaccgtcg
     1681 cgactggctc gattcggcga agcgcgcgtt cgccttcctc cgctcgacgc tgtggcgcga
     1741 cggccgcctg ctcgcgacct ggaaggacgg ccgcgcgcac ctcaacgcct acctcgacga
     1801 ctacgcctac ggggtcgcgg ccgccatcga gctgctgcag gccgagtacg acgccgacac
     1861 gctggccttc gccgaggagc tcgccgaggt cctgatcgag cagttcgagg acacggtgaa
     1921 cggcggcttc ttcttcacca gccatgacca cgagcggctc atccaccggc ccaagcccgg
     1981 cgacgacaac gcgacgccgt cgggcaacgg catggcggcg ctcgggctgc agcggctctc
     2041 gctcctcacc ggcgagacgc gctacgccgc gagcgccgag cgcgcgatcg cgcagttcta
     2101 tcccgagctg gccgcgcagc cgggcggcca cacgacgctg ctgatggcgc tcgaggagcg
     2161 cctcgtgccg ccgcgcaccg tgatcctccg cggcccggcc gggccgctcg tgcgctggcg
     2221 ccgcgcgctc gccgagcgtc acttgccggc gacgctcgtg ctcgcgctcg caccggacgc
     2281 gcgcgacctg ccgccggtgc tcgcgaagcc ggcgtcggat cgcgtcaacg cctgggtgtg
     2341 cgaaggcgtt acctgcctgc cgccggtcga ccgcctcgag gatctgatcc cgcaggtttc
     2401 caaggcggtc gaaatccatt agcatcgtgc cgcacatcca aggaggattc atgaataaga
     2461 agctggtctg gtttgccgca gcgggcgctc tggtctgcgc tcctgcgctc gcgcaggacg
     2521 cgcagcagct gctcaaggac aaggcgtgcc tcgcctgcca cagcctcgac aacaaggtcg
     2581 tcggtcccgc gtacaaggac gtcgcgaaga agttccgcgc gcgcaaggac gccgagtcgt
     2641 acctggcgaa gaagatccgc gaaggctcga ccggggagtg gggccagatt ccgatgccgc
     2701 cgaacacgac ggtgagcgag gccgaggcgc acacgctcgc gaagtacatc ctgtcgctga
     2761 agtaggaggg tcccgatggc tgccgtccgc gtcggatcgg tcctgatcct ggcctgcgcg
     2821 atcgcggggc tcgcggcctg cggcgacgac aagaaagcgg ccggcgcgaa gggcggggcc
     2881 acggtgacga tcggcatcgc ggcgccgctc accggaccgc agtcgcacat cggcaaggac
     2941 ctcgagaacg ccgtgcggct cgcggccgac gacgcgaacg cggccaaggt cgtcgtcggc
     3001 gggaagcccg tgcatttcga ggttcaggcc gaagacgacc aggccgatcc gaagatggcg
     3061 ccggtggtcg cgcagaagct cgccgatgcc cacgtcgccg cggtggtcgg ccacttcaac
     3121 tcggggacga cgatcccggc gtcgaggatc tacaacgacg ccggcatccc gcagatcagc
     3181 ccgtcggcca ccaatcccaa gtacaccgag cagggattca agaccgcgtt ccgcgtcgtc
     3241 gccaacgaca accagcaggg caaggtggtc ggcgagtaca tcgcgaagga cctgaagtcg
     3301 aaggcgctcg cgatcatcga cgaccgcacc gcgtacgggc agggcgtcgc cgacgtggtc
     3361 gagcgctcgg cgaaggcggc gggcgccgtc gtggtcgcga gggagttcac gaccgaccgc
     3421 tcgaccgact tcaaggccat cctcaccaag atcaagggca cgcggcccga tgtcatcttc
     3481 ttcggcggca tcgacacgca ggcggggccg atgctgcagc agatgcgcgg cctgggcatc
     3541 gaagcgcagt tcgtcggcgc cgacggcatc cagagcacgg agctgttcaa gctcggcggc
     3601 aacgccgtcg agggcacgct cgcatccttc ccggggctgc cgctcgaccg catgccgcag
     3661 ggcgcgggct tcggcgagaa gtaccggaag aagtggaacc aggacgtggt gctgtacgcg
     3721 ccgcagggct acgacgcctt caatatcttc gtcgaggcga tgaagcgggc gggctcgacc
     3781 gacccggcga agttcctgcc ggaagtcgcg aagaccgact acaacggcat taccggcccg
     3841 atccgcttcg acgagaaggg cgacctcgag gacggcccga tcaccatctt caaggcggtc
     3901 ggcggcaaat ggcaggcggt cgccacgatc ggcgggccgt cgcaggggca ggccgccaag
     3961 cccgcggaga agaactagcg cccgtggagc ttgtccgaat cgactagggt ggcacccttc
     4021 gggtgccgtt tggaatcggc acctgcgggt gccgttttct tatcttcgtt cgagacgcat
     4081 ggatatcctg ctgcagcaac tgatcaacgg cgtgacgctg ggcagcgtct acgccctggt
     4141 cgcgctcggg tataccatgg tctacggcat catcggcctg atcaacttcg cgcacggcga
     4201 gctcgtgatg gtgggcgcgc tgacggcgct ctcggtgatc gcagcgctgc tgcgcctggg
     4261 gctcggcctg ccgccggcgg cgatgatgct gatcgcgctg ctcgcggcgc tcccggtatg
     4321 catgctgctc gcgtacgcga tggagcgggt cgcgtaccgg ccgctgcgcc gcgcgccgcg
     4381 cctggcgccc ctgatcacgg cgatcggcgt gtcgatcgtg ctccagaacg tcgcgatgct
     4441 ggtctggggg cgcagctatt tcagcttccc gccgatcctc aacgtcacga actacgacat
     4501 cgcgggcgcg accatcacgg acctgcagat cgtgatcgtg ctgctctcgg cggcgctcat
     4561 ggccgggttg ctgctgatcg tgcaccgcac ccggctgggc ctcgcgatgc gcgcgaccgc
     4621 cgagaacctc ggcgtggccg cgctcatggg cgtggatgcg aaccgcatca tcgtcgccgc
     4681 gttcctgctc gggtccgcgc tcgccgcggt cgccggcctc atggtgtcgg cctactacgg
     4741 catcgcgcac tactacatgg ggttcctgct cgggctgaag gcgttcacgg cggcggtgct
     4801 cggcggcatc ggcaacatcg gcggcgcgat gctcggcggc cttctggtcg gtctcgccga
     4861 gaccttcacg agcgggtacc tgacttcgac cttccaggac ctcgtggtct tctccgttct
     4921 cg
//
LOCUS       DATDMW010000083         5516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2264606, whole genome shotgun sequence.
ACCESSION   DATDMW010000083 DATDMW010000000
VERSION     DATDMW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264606"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VI319_03540"
     CDS             complement(<1..909)
                     /locus_tag="VI319_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY6862949.1"
                     /translation="MGSFTAKHGWLAVACALAPGYACAAGLGKITVQSALGQPLRAEI
                     EVVSLQAGEAESLSAKLGAIAAFRQADIEFNPVLQSVKFSVEPRPGGKVVVLMSSTQP
                     INEPYVDMLVEVNWASGRLLREYTFLLDPPEYRSPTLTAAKQPSAAPAAEPAPAPAPA
                     RAAAPEPQAPAPQAAAPAEPAQAAPAESTAQSEPKEPAEGSEPAGAGGTTAQAAPEAP
                     KAPEEHELVSEPKPAATYEVKRGDTLSKIALRNKVDGVTLQQMLVALFRANQEAFVGD
                     NMNRLRAGKIITIPDKEEMAAVGAPDA"
     gene            complement(1068..2180)
                     /gene="asd"
                     /locus_tag="VI319_03545"
     CDS             complement(1068..2180)
                     /gene="asd"
                     /locus_tag="VI319_03545"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY6862950.1"
                     /translation="MKKVGFVGWRGMVGSVLMQRMAAERDLALVEPVFFSTSQVGAMG
                     PFVGKETAPLKDAKRIDELAAMDIVVSCQGGDYTNEIHPKLRAAGWKGYWIDAASALR
                     MKDDAVIILDPVNRAVIDRALERGVRDYIGGNCTVSLMLMAMQGLFQAGLVEWLTAMT
                     YQAASGAGAENMRELVKQMGFAHAAAKTLLDDPASAILELDRAVAQAIRSDALPRQHF
                     GVPLAGSLIPWIDKDLGNGQSREEWKGQAEGNKILGREAKPIPIDGICVRVGAMRCHS
                     QALTVKLARDVPMPEVERLLAGVNDWVRVVPNEKEASLRELTPAAVTGKLHVPIGRLR
                     KLPMGGEYLAAFTVGDQLLWGAAEPLRRMLRILADS"
     gene            complement(2196..3251)
                     /gene="leuB"
                     /locus_tag="VI319_03550"
     CDS             complement(2196..3251)
                     /gene="leuB"
                     /locus_tag="VI319_03550"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY6862951.1"
                     /translation="MKVAVLAGDGIGPEIVAEALKVLRKLDLRLDFDEAPVGGAGYEA
                     SGDPLPSATLALARSADAILFGSVGGPKWDALERPKRPEQALLRLRKELGLFANLRPA
                     IVYPELAEASTLRPEVVAGLDILILRELTGDIYFGQPRGIRALPAGGREGFDTMRYSE
                     AEIERIAHVGFRAAAKRRRKLCSVDKANVLETSQLWRDVVTRVGREYPAIELSHMYVD
                     NAAMQLVRDPRQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDASNKGLYEPIHG
                     SAPDIAGKGIANPLATILSAAMMLRYSLNLDAQAARIEAAVKKALAKGLRTGDIHRPG
                     TTRVGTREMGDAVAASL"
     gene            complement(3248..3892)
                     /gene="leuD"
                     /locus_tag="VI319_03555"
     CDS             complement(3248..3892)
                     /gene="leuD"
                     /locus_tag="VI319_03555"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY6862952.1"
                     /translation="MEKFTVLDGLVAPMDKANVDTDAIIPKQFLKSIRRTGFGPNLFD
                     AWRYLDEGRPDKPNEGRPLNPDFVLNQPRYRGAKILLARENFGCGSSREHAPWALLEY
                     GFRAVIAPSYGDIFYNNCFKNGLLPVVLKASEVDALFHDVAAFPGFRLVVDLERQAVA
                     TADGAKAFRFEVDPFRKYCLLNGLDDIGLTLRHADKIHAFEEKRRAEQPWLQTS"
     gene            complement(3895..5301)
                     /gene="leuC"
                     /locus_tag="VI319_03560"
     CDS             complement(3895..5301)
                     /gene="leuC"
                     /locus_tag="VI319_03560"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY6862953.1"
                     /translation="MTPQTLYDKLWQSHVVHVEDDGTTLLYIDRHLVHEVTSPQAFEG
                     LKTSGRRLWRVAANLAVADHNVPTTDRSRGIADPVSRLQVDTLDRNCDEHGIVEFKMN
                     DRRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGCLAFGIGTSEVEHVMATQCLI
                     AKKMKNMRVSVEGLLPRGVTAKDLALAVIGRIGTAGGTGYTIEYSGSTISALSMEGRM
                     TLCNMAIEAGARAGMVAVDDTTIRYLKGRPFAPRDAMWDRAVAHWRTLKSDEGAAFDR
                     VVQIDARALKPQVTWGTSPEMVVTVEDRVPDPDKERDSVKREGMERALQYMGLQPNTA
                     ITEIRIDKVFIGSCTNSRIEDLRAAAGVVRGRRVAPSVRLALVVPGSGIVKAQAESEG
                     LDRVFRDAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGTGGRTHLVSPE
                     MAAAAAIAGHFVDVRRWS"
BASE COUNT          811 a         1918 c         1868 g          919 t
ORIGIN      
        1 cgcgtccggc gccccgaccg cggccatctc ttccttgtcg gggatggtga tgatcttgcc
       61 ggcacgcagc cggttcatgt tgtcgccgac gaaggcttcc tgattggcgc ggaacagcgc
      121 gaccagcatc tgctgcagcg tgacgccgtc gaccttgttg cgcagcgcga ttttcgagag
      181 cgtgtcgccg cgcttgacct cgtaggtcgc ggccggtttc ggttcggaga cgagctcgtg
      241 ctcttcgggt gccttcggcg cctcgggcgc cgcctgggcg gtggtgccgc cggcgccggc
      301 gggctcgctc ccctcagccg gctctttcgg ctcggattgc gcggtcgact ccgcaggcgc
      361 cgcttgagca ggctcggcgg gcgccgccgc ctgtggggcg ggcgcctgcg gctctggagc
      421 ggccgcgcgc gcgggagcgg gagcaggagc gggctccgcg gccggcgccg cgctcggctg
      481 cttcgcggcg gtcagcgtcg gcgagcggta ttccggcgga tccagcagga acgtgtattc
      541 gcgcagcagc cgcccgctcg cccagttcac ctcgacgagc atgtcgacgt agggttcgtt
      601 gatgggctgc gtcgagctca tcagcacgac gactttgccg ccggggcgtg gctcgaccga
      661 gaacttcacg ctctgcaaca ccggattgaa ctcgatatcg gcctgccgga acgccgcgat
      721 cgccccgagc tttgcgctca ggctctcggc ttcgccggcc tgcagggaaa cgacttcgat
      781 ctcggcgcgc agcggctgac cgagggcgga ctgaacggtg atcttcccga ggccggccgc
      841 gcacgcgtag cccggcgcga gcgcgcaagc gaccgcaagc caaccgtgtt tggcggtgaa
      901 gctccccact ctacccaccc tgaaagaccc taaggaggaa actaaaatac catcatgagc
      961 ttagccttgc aagctcaagg cccacatcaa aaatctcgtc aagtggctat tttattaccg
     1021 ctttccactt gacttggggc ccagatcggg ttacccccgg ccttttttca cgaatcggcg
     1081 aggatgcgca gcatccggcg cagcggctcg gcggcgcccc acagcagctg atcgccgacg
     1141 gtgaaggcgg cgagatattc gccgcccatc ggcagcttgc gcaggcggcc gatcggcacg
     1201 tgcagcttcc cggtgaccgc ggcgggcgtg agctcgcgca ggctcgcttc tttttcgttc
     1261 ggtaccacgc gcacccagtc gttcacgccc gcgagcagcc gctcgacctc gggcatcggt
     1321 acgtcgcgcg cgagcttcac cgtgagcgcc tggctgtggc agcgcatcgc gcccacgcgc
     1381 acgcagatac cgtcgatcgg gatcggcttc gcctcgcggc cgaggatctt gttgccctcg
     1441 gcctggcctt tccattcttc gcggctctgg ccgttgccga ggtccttgtc gatccacggg
     1501 atgaggctgc ccgcgagcgg cacgccgaaa tgctgtctcg gcagcgcgtc gctgcggatc
     1561 gcctgggcga ccgcgcggtc gagctcgagg atcgcggagg ccggatcgtc gagcagggtc
     1621 tttgccgcgg cgtgcgcgaa tcccatctgc ttcaccagct cgcgcatgtt ctcggcgccc
     1681 gcgcccgagg cggcctggta ggtcatcgcg gtgagccact cgaccagccc ggcctggaac
     1741 aggccctgca tcgccatgag catgaggctc acggtgcagt tgccgccgat gtagtcacgc
     1801 acgccgcgct cgagcgcgcg gtcgatcacc gcccggttga ccggatcgag gatgatgacg
     1861 gcgtcgtcct tcatgcgcag cgccgaggcg gcgtcgatcc agtagccctt ccacccggcg
     1921 gcacgcagct tcggatggat ctcgttcgtg tagtcgccgc cctggcagga cacgacgatg
     1981 tccatcgcgg ccagctcgtc gatgcgcttc gcatccttga gcggcgcggt ctccttgccg
     2041 acgaacggcc ccatcgcgcc gacctgcgag gtggagaaga acacgggctc gaccagcgcg
     2101 agatcgcgct cggccgccat gcgctgcatg agcaccgagc ccaccatccc gcgccaaccg
     2161 acgaaaccga ctttcttcat gattctccta tgctatcaga gcgacgccgc cacggcgtct
     2221 cccatttcgc gcgtgccgac gcgcgtcgtt cccgggcggt ggatgtcgcc cgtgcgcagg
     2281 cccttcgcca gcgccttctt caccgccgct tcgatccgcg ccgcctgcgc gtcgaggttc
     2341 aggctgtagc gcagcatcat cgccgccgaa aggatggtcg cgagcggatt ggcgatcccc
     2401 ttgcccgcga tatcgggcgc cgagccgtgg atcggctcgt agagcccttt gttgctcgcg
     2461 tcgagcgacg ccgacggcag catgccgatc gatccggtca gcatcgacgc ctcgtcggag
     2521 agaatgtcgc cgaacatgtt gccggtgacg atcacgtcga actggcgcgg atcgcgcacg
     2581 agctgcatcg cggcgttgtc gacgtacatg tgcgagagct cgatcgcggg atactcgcgg
     2641 ccgacccggg tcacgacgtc gcgccagagc tgcgaggtct cgagcacgtt ggccttgtcc
     2701 accgagcaca gcttccggcg ccgcttcgcg gccgcgcgga acccgacgtg agcgatgcgc
     2761 tcgatctcgg cctcgctgta gcgcatggtg tcgaagcctt cgcgtccgcc cgcgggtagc
     2821 gcgcggatgc cgcgcggctg cccgaagtag atgtcgcccg tcagctcgcg caggatcagg
     2881 atgtcgaggc cggcgacgac ctcggggcgg agggtcgagg cctcggcgag ctccggatag
     2941 acgatcgccg ggcgcaggtt cgcgaacagc ccgagctcct tgcgcaggcg caggagcgcc
     3001 tgctcgggac gcttgggccg ctcgagcgcg tcccacttgg gcccaccgac cgaaccgaac
     3061 agaatcgcgt ctgcggatcg cgcgagcgcc agcgtggccg aggggagcgg gtcgccgctg
     3121 gcctcgtagc ccgcgccgcc gaccggcgct tcatcgaaat cgagccgcag atcgagcttg
     3181 cgcaggacct tcagcgcctc ggcgacgatc tcgggcccga tgccgtcgcc cgcgaggacg
     3241 gcgaccttca cgacgtctgc agccagggct gctcggcccg gcgcttctcc tcgaaggcgt
     3301 ggatcttgtc ggcgtggcgc agcgtgaggc cgatgtcgtc gagcccgttc agcaggcagt
     3361 atttgcggaa cggatcgacc tcgaagcgga acgccttcgc gccgtcggcg gtcgcgaccg
     3421 cctggcgctc gaggtcgacc acgaggcgaa agcccggaaa cgccgcgacg tcgtggaaca
     3481 gcgcatcgac ctccgaggcc ttgagcacga ccggcagcag cccgttcttg aagcagttgt
     3541 tgtagaagat gtcgccgtac gacggcgcga tcaccgcgcg gaagccgtac tcgagcagcg
     3601 cccagggcgc gtgctcgcgc gaggagccgc agccgaagtt ctcgcgcgcg agcaggatct
     3661 tcgcgccgcg gtagcgcggc tggttcagga cgaagtccgg gttgagcggc cgcccctcgt
     3721 tcggcttgtc ggggcggccc tcgtcgagat agcgccaggc gtcgaacagg ttcggcccga
     3781 agcccgtgcg ccggatcgac ttcaggaact gcttcgggat gatggcgtcg gtgtcgacgt
     3841 tcgccttgtc catcggcgcg acgagaccgt ccagcacggt gaacttttcc atgatcagct
     3901 ccagcggcgc acgtcgacga aatggcccgc gatcgccgcc gcggccgcca tctccgggct
     3961 caccaggtgc gtgcgcccgc ccgtgccctg gcgcccctcg aagttgcggt tcgaggtcga
     4021 ggcgcagcgc tcgccgggct cgagccggtc ggcgttcatc gcgaggcaca tcgagcagcc
     4081 gggctcgcgc cactcgaagc ccgcgtcgcg aaaaacgcgg tcgaggccct cgctctcggc
     4141 ctgcgccttg acgatgcccg atccgggcac gaccagcgcg agacgcacgc tcggcgcgac
     4201 gcgcctgccg cgcaccacgc ccgcggcggc tctcaggtcc tcgatgcggg aattcgtgca
     4261 ggagccgatg aacaccttgt cgatcctgat ttcggtgatc gccgtgttgg gctggaggcc
     4321 catgtactgc agcgcgcgct ccatgccctc gcgcttcacc gaatcccttt ccttgtcggg
     4381 atcgggaacg cggtcctcga ccgtgaccac catctcgggc gaggtgcccc aggtcacctg
     4441 cggcttgagc gctcgcgcgt cgatctggac gacgcggtcg aaggccgcgc cctcatcgct
     4501 cttcagcgtg cgccaatgcg ccacggcgcg gtcccacatc gcgtcgcggg gcgcgaacgg
     4561 ccgccctttg agatagcgga tcgtggtgtc gtcgaccgcg accatgcctg cgcgcgcgcc
     4621 cgcctcgatc gccatgttgc acaaggtcat gcgcccttcc atggagaggg cgctgatggt
     4681 cgaccccgaa tattcgatgg tgtacccggt tccgcccgcc gttccgatcc ttccgataac
     4741 ggccagcgcg agatccttcg cggtgactcc acgaggcagg aggccttcca ccgagacgcg
     4801 catgttcttc atcttcttcg cgatcaggca ctgggtcgcc atcacgtgct cgacctcgga
     4861 ggtgccgatc ccgaacgcga ggcacccgaa ggcgccgtgc gtgctcgtgt gcgagtcgcc
     4921 gcagacgacc gtcatcccgg gcagcgtcgc gccctgctcg ggcccgatca cgtggacgat
     4981 gccctggcgc cggtcgttca tcttgaactc gacgatcccg tgctcgtcgc agttgcggtc
     5041 cagcgtgtcc acctgcaatc gcgacaccgg gtccgcgatt ccccgcgagc ggtcggtggt
     5101 cgggacgttg tggtcggcga ccgcgaggtt ggccgcgacg cgccacagcc tgcggccgga
     5161 cgtcttcagg ccttcgaagg cctgcgggct ggtcacctcg tgcacgaggt gccggtcgat
     5221 gtagagcagc gtcgtgccgt cgtcctcgac gtgaaccacg tgcgactgcc agagcttgtc
     5281 gtagagcgtc tgcggcgtca tggcgaaaat ctcaatcgga accggaaatt atagccgatc
     5341 gcgaccgcct cacccgccgc tcgccgcgga aagcgaatat agtaggtcca cttcacggaa
     5401 gatccgcaca tgagcaggcc gcaaggccgc cctcgggggg gaagcagcgc aggatcgggc
     5461 gaggtgcgcg gcgacgcgcg cccggcggcg cgcgcgcgcg ccggcctcgc cgagga
//
LOCUS       DATDMW010000084         2210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1517884, whole genome shotgun sequence.
ACCESSION   DATDMW010000084 DATDMW010000000
VERSION     DATDMW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517884"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2210)
                     /gene="malQ"
                     /locus_tag="VI319_03565"
     CDS             complement(<1..>2210)
                     /gene="malQ"
                     /locus_tag="VI319_03565"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY6862954.1"
                     /translation="YLDVWKRPHRAPQDTKLALLRAMGVDAGDGDGAQAAAALAEAEE
                     RRWREALPAATVVRERESPLRVALVLGADADAEKIDWLLELENGERVAGALRAGTLRE
                     LEARDIGGARRVRYALELPIAPEAGYHWFSLRESGAPQRVLASTRLISCPARCFQPAG
                     FAGDYENAGRVWGPALQLYAVRSRRNWGIGDYTDLAEGIEAAAALGAGIVGVNPLHAL
                     FPTRPEDASPYSPSSRLALNVLYLDVERIPELAESDDERARVAAPGFQATLRALREKP
                     LVDYAGVARVKLDVLERLYRRFRETALAGDRGRAFRAFQAEGGERLRLHALFEALQER
                     FVAEDPGVWGWPAWPEPYRDPASPEVERFAREHLERVELWQWLQWHAQTQVARAQHRA
                     IELGMPVGLYLDLAVGSHPGGAETWIQRGLYATQASVGAPPDEFNLKGQDWGLPPVIP
                     QRLAQARYEGFTALLRANMKNAGALRIDHVMALMRLFWVPPQASPKEGTYVDYPFADL
                     LGILCLESVRNRCIVVGEDLGTVPEEVFTALREHGILSYRPIWFERDGESAFQAPERY
                     PAQALVTVSTHDLPTLRGFWRGADLETRHRLHLFPDEQVRARQLDDRSQDRARLVQAL
                     ERAGLAPPGAAPGRGDTDEAFMVAVHAYLARTPAKLLTFQMEDVFGEVEQVNLPGTTE
                     ERHPNWRRKLAEDLEEWARDARVLALVAALRRERGPAEPRERPRRPDAPAAALA"
BASE COUNT          277 a          830 c          826 g          277 t
ORIGIN      
        1 cgcgagcgcg gccgcgggcg cgtcgggccg ccgcggccgc tcgcgcggct cggcggggcc
       61 gcgctcgcgc cgcagcgcgg cgaccagcgc gaggacgcgc gcgtcgcgcg cccactcctc
      121 caggtcctcg gcgagcttgc gccgccagtt cgggtgccgc tcctcggtcg tccccgggag
      181 gttcacctgc tcgacctcgc cgaacacgtc ctccatctgg aacgtcagca gcttcgccgg
      241 cgtgcgcgcg agatacgcgt gcacggccac catgaacgct tcgtcggtgt cgccgcgccc
      301 gggcgccgcg cccggcggcg cgagcccggc gcgctcgagc gcctgcacca ggcgggcgcg
      361 gtcctggctt cggtcgtcga gctggcgcgc gcgcacctgc tcgtcgggaa agagatgcag
      421 gcgatgccgc gtctcgagat cggcgccgcg ccagaaaccg cgcagcgtcg gcaggtcgtg
      481 cgtgctcacc gtgaccagcg cctgcgccgg gtagcgctcg ggcgcctgga aggcgctttc
      541 gccgtcgcgc tcgaaccaga tcggccggta cgagaggatg ccgtgctcgc gcagggcggt
      601 gaacacctcc tcgggcacgg tgccgaggtc ctcgccgacg acgatgcagc ggttgcgcac
      661 gctctcgagg cacaggatgc cgagcaggtc ggcgaaggga taatcgacgt aggtgccttc
      721 cttcggcgag gcctgcggtg gcacccagaa caggcgcatc agcgccatta cgtgatcgat
      781 gcgcagcgcg ccggcgttct tcatgttcgc gcgcagcagc gcggtgaagc cctcgtagcg
      841 cgcctgcgcc aggcgctgcg ggatcaccgg cggcaggccc cagtcctggc ccttgaggtt
      901 gaactcgtcg ggcggcgcgc cgacgctcgc ctgcgtcgcg tacaggccgc gctggatcca
      961 cgtctccgcg ccgcccgggt gcgagccgac cgcgaggtcg aggtagaggc cgaccggcat
     1021 cccgagctcg atcgcgcggt gctgggcgcg cgcgacctgc gtctgcgcgt gccactgcag
     1081 ccattgccag agctcgacgc gctcgaggtg ctcgcgcgcg aagcgctcga cctcgggcga
     1141 ggccggatcg cggtacggct cgggccacgc gggccatccc cacacgccgg gatcctcggc
     1201 gacgaagcgc tcctgcagcg cctcgaacag ggcgtgcagc cgcaggcgct cgccgccctc
     1261 ggcctggaag gcgcggaacg cgcgtccgcg gtcgcccgcg agcgcggtct cgcggaagcg
     1321 gcggtagagg cgctcgagca cgtcgagctt gacgcgcgcg acgccggcgt agtcgacgag
     1381 cggcttctcg cgcagcgcgc gcagcgtcgc ctggaacccg ggcgcggcga cccgcgcgcg
     1441 ctcgtcgtcg gactcggcga gctcggggat gcgctccacg tcgaggtaga ggacgttgag
     1501 cgcgaggcgg ctcgacgggc tgtacgggct ggcgtcctcc ggacgcgtcg gaaacaaggc
     1561 gtgcagcgga ttcacgccca cgatgccggc gccgagcgca gccgccgctt cgatcccctc
     1621 ggccagatcg gtatagtcgc cgatgcccca gttgcgccgc gagcgcaccg cgtagagctg
     1681 cagcgcgggt ccccagaccc ggcccgcatt ttcgtagtcc cccgcgaacc ccgccggctg
     1741 gaagcagcgc gccgggcacg agatcagccg cgtcgacgcg agcacgcgct gcggcgcgcc
     1801 ggactcgcgc aggctgaacc agtgatagcc ggcctcgggc gcgatcggca gctcgagcgc
     1861 gtagcggaca cggcgcgcgc cgccgatgtc gcgcgcctcg agctcgcgca gcgtcccggc
     1921 gcgcagcgcg cccgcgacgc gctcgccgtt ctcgagctcg agcagccagt cgatcttctc
     1981 ggcgtcggcg tcggcgccga gcacgagcgc gacgcgcagc ggcgactcgc gctcgcgcac
     2041 cacggtggcc gcgggcagcg cctcgcgcca gcgccgctcc tcggcctcgg cgagcgcggc
     2101 cgcggcctgc gcgccgtcgc cgtcgccggc gtcgacgccc atcgcgcgca gcagcgcgag
     2161 cttggtgtcc tgcggcgccc ggtgcgggcg cttccagacg tcgagatagg
//
LOCUS       DATDMW010000085         2176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_149180, whole genome shotgun sequence.
ACCESSION   DATDMW010000085 DATDMW010000000
VERSION     DATDMW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149180"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..156
                     /locus_tag="VI319_03570"
     tRNA            84..156
                     /locus_tag="VI319_03570"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:117..119,aa:Val,seq:tac)
     gene            481..1092
                     /locus_tag="VI319_03575"
     CDS             481..1092
                     /locus_tag="VI319_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEY6862955.1"
                     /translation="MDEVRHLRVLIANEKRERLELLAQVVAGLGHEVIAREIYVKEVG
                     AVTARERPDVALVGLGLHSEHALELITEIVREASCPVIALLSAKDPAYVHEAAKRGVF
                     AYIVDGSPEELQSAIDITLQRFAEYHSLQGAFGRRATIEQAKGILMARHAISAARAFE
                     MLRDHSQHNGHKLVDIAQAIVDSHLLLLPPAQTPAASSSSPPE"
     gene            1277..1579
                     /locus_tag="VI319_03580"
     CDS             1277..1579
                     /locus_tag="VI319_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862956.1"
                     /translation="MVEIRVVLPDAAEALGLLRRLAALFDRSSVSFDRSLNEVRVRSE
                     WESRSILAVIDTVQSWLTDDDVDSAELSVGDRSYAMVGPRLVPASERAPAGIGGAV"
     gene            1576..2016
                     /locus_tag="VI319_03585"
     CDS             1576..2016
                     /locus_tag="VI319_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY6862957.1"
                     /translation="MIFGTPTWALWAIAASLLAIGELFTPGLFFLGPLAVAALVAAIV
                     AGLAAGAVGGLLAFVGAAAICVMVLRPLARRHIRLPAVSRTGTAALVGRKALVLELVD
                     DSGGRVKIGGEEWSARPYLQGEALPAGEQVEVAQIEGATALVLR"
BASE COUNT          320 a          741 c          750 g          365 t
ORIGIN      
        1 ctcgtctcgg gcagcgcgag gcagatcgcg cggacgcgcg cgatggcgtc gcgtttggcc
       61 acgtgtaccg tgttatcacg cccgggcggt tagctcagct gggagagcac ctgccttaca
      121 agcagggggt cggcggttcg atcccgtcac cgcccactac cgaatccccg cgatcggcgg
      181 gttcttgttc gggcgagtgc cgcaatgaac gggcgtcgtt gctcgcccgt ccgagccctc
      241 gtcctcgaga gttcccggcc tgccggcttc caagatcgcc ccttccgcac gagctcgact
      301 accgacctga ggctgcggtc gaccaaccaa ctccggccca tgctcttgag gcggatctgt
      361 ttctggcccg cagatgttgg cggcacccca gtgcatacgt cgccttccga tcgaactcga
      421 acgcgcagtt gcgccaagag ccgaagccca aaggccgcgc tacatttgcc gacaatggcc
      481 gtggacgagg ttcggcatct gcgcgtcctg atcgcgaatg agaagcggga gcggctcgag
      541 ttgcttgcgc aggtggtggc cgggctgggg catgaggtga tcgcccgcga gatctacgtg
      601 aaagaggtcg gcgcggtgac cgcgagggag cgaccggatg tggccctcgt cgggctcggc
      661 ctgcattccg agcacgcgct cgagctgatc accgagatcg tccgcgaggc gtcctgtcct
      721 gtgatcgcgc tcctctcggc caaggacccg gcgtacgtgc acgaggcggc gaagcgcggc
      781 gtcttcgcct acatcgtcga cggcagcccc gaggagctgc agagcgcgat cgacatcacc
      841 ttgcaacggt tcgccgagta ccacagcctg cagggcgcgt tcgggcgtcg ggcgacgatc
      901 gagcaggcga aagggatcct gatggcccgc cacgcgatca gtgccgcgcg ggcattcgag
      961 atgctgcgcg accattcgca gcacaacggc cacaagctcg tcgacatcgc ccaggcgatc
     1021 gtcgacagcc acctgctgct gctgccgccc gcgcagacac ccgcagcatc ctcgagctca
     1081 ccgcccgaat gaacgggcga tcccgtcggt gctaggctta cggctgcagc gccatagcga
     1141 tcggcgcagc gagtcaccgc gcggcgctta gcacgaaacg ccgcgggaga tcctcccaca
     1201 gcgacgcggg ttggagccgt atcgggccgg cctcatcctc gcggtcggcg aagacgcttc
     1261 gagccgggaa ggtcgaatgg tcgagatccg cgtcgtgcta ccggacgccg ccgaggcgct
     1321 cggcctgctg cggcgcctcg cagcactgtt cgaccgctcg tcggtctcct tcgaccggtc
     1381 cctgaacgag gtccgggtac ggtccgaatg ggaatcgcgc tcgatcctcg cagtgatcga
     1441 cacggtccag tcatggctca ccgacgacga cgtcgactcg gccgaactgt cggtcggcga
     1501 ccgctcgtac gcgatggtcg gcccgaggct tgtaccagcg agcgaacggg caccggccgg
     1561 catcggaggc gcggtttgat cttcggcact ccgacctggg cgctctgggc aatcgcggcc
     1621 tcgctgctcg cgatcggcga gctgttcacg cccggcttgt tcttcctcgg tcctcttgcg
     1681 gtggcggcgc tggtcgccgc gatcgtcgcc ggactggccg cgggcgctgt cggcgggctg
     1741 ctcgcattcg tcggggcggc ggcgatctgc gtgatggttc ttcgcccgct cgcgcggcgc
     1801 cacatccggc tgcccgcggt cagccgcacg ggcacggccg cgctcgtggg aaggaaggcg
     1861 ctcgtgctgg agctggtcga cgactccggc ggccgggtga agatcggcgg cgaggagtgg
     1921 tccgcgcgtc cgtatctcca aggggaagcg ttgccggcgg gggaacaggt cgaagtcgca
     1981 cagatcgagg gcgccacggc gctcgtccta cgttgaacgg gagaaaacca tggtcgctct
     2041 gatcgtcttg ctcgccgtcg ccgcgttggt tctgctcgtc gtcgcgcgca cgatccgcat
     2101 cgttccgccg gcgcgcgcgg gcgtgatcga acgcttcggc cgctaccagc gcacgctctc
     2161 tcccggtctc gcgctc
//
LOCUS       DATDMW010000086        10158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1143789, whole genome shotgun sequence.
ACCESSION   DATDMW010000086 DATDMW010000000
VERSION     DATDMW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143789"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VI319_03590"
     CDS             complement(<1..769)
                     /locus_tag="VI319_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6862958.1"
                     /translation="MDLAVHGRRAYAYTGGVPFDASLRTVVFVHGGALDHSVWGLQTR
                     YLAHHGRAVLAPDLPGHGRSEGPALRRIEEIAEWLIALLDAVGVARAALVGHSMGSLV
                     ALDCAARFPERVEALALIGSAFPMRVSAELLEATRSDEPRARAMINAWSHGGYAQFPG
                     NPGPGSWVIGANLRLMQRAKPGVLHADFSACNDYTEGLDRAAQVRCPALLLSGRRDQM
                     TPARSARALASAIAGAEWVELDRCGHNVMAEKPDELLD"
     gene            complement(769..2064)
                     /locus_tag="VI319_03595"
     CDS             complement(769..2064)
                     /locus_tag="VI319_03595"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEY6862959.1"
                     /translation="MAAPRPLQFDTLTLHAGQQPDPLTGARATPIYFTTSFVFRDADH
                     AAALFDMERAGHVYSRISNPTNAVLEERIAALEGGVGAIATASGQAALHLAIATLMGA
                     GSHIVASRSLYGGSHNLLSYTLPRFGIETTFVDPRDLGAWRDAVRPSTRLFFGETLGN
                     PGLDVLDVAAVAELAHAAKVPLLVDSTFTTPYLLRPFDHGADLVVHSATKFLGGHGVA
                     IGGVLVDGGSFDWDASGKFPELTEPYGGFHGMDFTEESTVAAFLLRARREGLRDFGAC
                     MSPATAFQILQGVETLPLRMQRHVENARKVIAFLSGHPAVASIAYPELPSHPDHALAR
                     RLLPRGCGAVFSFNLEGDRAAGRRFIESLRVFSHLANVGDAKSLVIHPASTTHFRMDA
                     EALRAAGITEGTVRLSIGLEDAEDLIEDLDRGLAAAAKA"
     gene            complement(2168..3646)
                     /locus_tag="VI319_03600"
     CDS             complement(2168..3646)
                     /locus_tag="VI319_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY6862960.1"
                     /translation="MVNSKLPIVVAACLGAATLLGYAGGRLAPITASPAATAQQKQEA
                     QQTQQPPVSQATRPLPDFSTLVEQYGPAVVNISTTQKVSAQDRLQGMDPNDPLYEFFK
                     RFNIPIPEGNSVRQGLGSGFIVSHDGYILTNAHVVADATQVTVKLTDKREFKAKVIGV
                     DKRSDVGLVKIEASDLPVVRIGDVSKAKVGEWVAAIGSPFGLDNSVTAGIISAKARSL
                     PDESYVPFLQTDVAINPGNSGGPLFNLNGEVIGINSQIYSRTGGYMGLSFAVPIDLAM
                     KVKEDLQKYGRVERGRLGVTVQPMTKELAQAFGIKQTDGALVSSVEPGSPAAKAGFQA
                     GDVITRVNDKPVEQSSDLPRVIGETKPGTSITLSIMRQGQPHDLRVQVGRAPDEEKVA
                     KADAQEKEQAKPQGKLGVAVRPLTGEERKQLGTESGVIVENATGPAAKAGIQPGDVIL
                     GVNNQPVKNIDQLRELVDRAKGNVALLVQRENARVYVPVPLG"
     gene            complement(3780..4472)
                     /locus_tag="VI319_03605"
     CDS             complement(3780..4472)
                     /locus_tag="VI319_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY6862961.1"
                     /translation="MGKIKNIREWTLFVLVGGTALLANLPEDWLAALSVERNVLLALL
                     ALIVFIALFLYLKFQFFFLVVLLAVAANMPADVADKLGVATAPLVIAMIAMVGISLIN
                     YVVGILPTGLETKPKERSVEGIKAIFYAIEKGNLVYAQKLLSKNFDPNLRAENGYTPL
                     MYATLRGDAKMVELLLRNGADVSLVSKSGDTAIELALSGGFPECANILKQARAEQQAR
                     EEALRARQAAAT"
     gene            4573..5127
                     /gene="greB"
                     /locus_tag="VI319_03610"
     CDS             4573..5127
                     /gene="greB"
                     /locus_tag="VI319_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY6862962.1"
                     /translation="MSKAFTRENDDALEDEPELEAQQPKGVKNYITPAGHARLKSELK
                     TLVEVERPELVKTVAWAASNGDRSENADYIYGKRRLREIERRIRYLMKRLEIAEVVDP
                     RDQDQDRVFFGATVVYRDGKGQEQTVSIVGTDEVDPAHRRVSWISPIAKALLKAREGD
                     AVTVNTPSGAEELEILEVRYDELR"
     gene            complement(5131..7899)
                     /locus_tag="VI319_03615"
     CDS             complement(5131..7899)
                     /locus_tag="VI319_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY6862963.1"
                     /translation="MKLASLAALLLGLLPAVAAPAGPAPAGAAAPSRVSPAPAKVVSI
                     EGVTEYRLANGLKLLLVPDPSIDTITVHITYLVGSRNEGYGEAGMAHLLEHMLFRGTP
                     RYPNVKGEFLKHGARFNGTTSYDRTNYFETFTADDKNLEWALDIEADRMRNARVSRKD
                     LDAEMTVVRNEFESGENNPYAVLRERVASAAYVWHNYGRAIIGARSDIENVPIERLQA
                     FYREFYEPDNAVLIVSGRFDEAAALRLVQKHFGPLPRPARALRKTYTVEPTQDGERTV
                     TLRRVGDAPLVSVLYHLPPGTHPDYAAVDVLVAMLNHVPSGRLHKALVETGKASYVFG
                     SERQQREAGYGYFGAGLPPVGSLDDARDVLLATLEEVGRSRPSDDEVERARTRLLNDI
                     ELSIADSRQLTGALSEYAAIGDWRMLYLHRDRLRKVTPEDVQRVAAHYLKASNRTVGL
                     FIPTKELDRAEIPAVPDVAAALKDYRGSGAVVQGEAFDPTPANIEARTIRGTLPGGLK
                     LALLPKRTRGHTVVAQISLRWGTEQSKVGRSAACGAASAMLVRGTQKKTREQIRNEFD
                     RLKANVGIGAEGGSIETVRESLPDVLRQVAEVLREPSFPASEFEQFRRAALTSIDTQR
                     SDPGALAGLAISRHLNPYPPGHWLYNMTLEERAAKLKDLSLEDVKRCYTDLVGASNGE
                     IAVVGDFDPDEVTRLLGQLLGDWKSPGPYSRIAHPYRDVSAINEAILTPDKANAVYRA
                     GMNLKIRDDNPDFPALLLGNYLLGGGSDSRLLRRVREQEGLSYSVGSYVSADSLDQHG
                     AFGVYAIYAPQNRARVEALIMEELRKALDQGFGAEEVENAKRGLLQARKVARTQDDAL
                     ASRLASYLFVGRTFDWDAQLERRIAGLTPAEVQAALRRYVDPAKLSVVKAGDFPKVAG
                     ASEPAPAN"
     gene            8086..10017
                     /gene="htpG"
                     /locus_tag="VI319_03620"
     CDS             8086..10017
                     /gene="htpG"
                     /locus_tag="VI319_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY6862964.1"
                     /translation="MTTTTAKETLGFQAEVKQLLDLMIHSLYSNKEIFLRELISNASD
                     AADKLRFEALSDNALYGSDADLRIRVSFDKSARTITVSDNGIGMSRDEVIRNIGTIAK
                     SGTREFFQSLTGDQAKDARLIGQFGVGFYSSFIVADRVTLVTRRAGAPAGEAVRWESG
                     GAGEYTIESVEKERRGTEVVLHLREGEDELLSDLRLKTIIRKYSDHIAIPIVMEKTRW
                     DPEKNDYVATGEDETVNQASALWARPKSEVTDEQYVEFYTHVAHDFEAPLAWTHNRVE
                     GRQEYTQLLYVPARAPFDLWDRHQRRGLKLYVRRVFIMDDAEQLLPAYLRFVRGVVDS
                     NDLPLNVSREILQESKDIDAIRSGCTKRVLGLLEDLAENRKDQYTAFWKEFGRVLKEG
                     VGEDAVNKERLAKLLRFASTHSDSEEQSVSLSDYVARMKEGQGGIYYVTADSFLAAKN
                     SPHLEIFRKRGIEVLLLHERVDEWLVANLPEHEGKPFVSVARGELDLGRIRTDEEKKE
                     EERLAGDLREVVEKLKAALGDRVKDVRVTSRLTGSPSCLVADEHDLGGNLGRILKAAG
                     QKVPTARPILEINPGHAIVQRLKQEQNRFGDWAHVLFDQALLAEGGQLDDPAGFVRRS
                     NELMLELAGSGPKLWTPGS"
BASE COUNT         1569 a         3426 c         3563 g         1600 t
ORIGIN      
        1 ggtcgagcag ctcgtcgggt ttctccgcca tgacgttgtg cccgcaccgg tcgagctcga
       61 cccactcggc gcccgcgatc gcggacgcca gcgcgcgcgc gcttcgcgcc ggggtcatct
      121 gatcacggcg acccgacagc agcagcgccg ggcagcgcac ctgcgccgcg cgatcgagcc
      181 cttcggtgta atcgttacac gcggagaagt cggcatggag cacgcccggc ttcgcgcgct
      241 gcatcagcct caggttcgcg ccgatcaccc acgagcccgg tccgggattc cccgggaact
      301 gcgcgtagcc gccgtgcgac caggcgttga tcatggcgcg cgcgcggggc tcgtcgctgc
      361 gcgtagcttc gagcagctcg gcgctcacgc gcatggggaa ggccgagccg atcagggcga
      421 gcgcctcgac gcgctcgggg aaccgagcgg cgcaatcgag cgcgaccagc gaacccatgc
      481 tgtggccgac gagggcggcc cgggcgacgc cgaccgcgtc gagcagcgcg atcagccatt
      541 ccgctatttc ttcgatcctg cgcagagcgg gtccttcgct gcggccgtgc cccgggaggt
      601 cgggcgcgag cacggcccgg ccgtgatgcg cgaggtagcg agtctgcaat ccccagacgc
      661 tgtgatcgag cgcgccaccg tggacgaaga ccacggtccg cagcgacgca tcgaagggca
      721 cgcctcccgt gtacgcgtac gcgcgcctgc cgtgaacggc gagatccatc acgccttcgc
      781 cgcggcggca aggccccggt cgaggtcctc gatgagatct tccgcgtcct cgaggccgat
      841 cgaaagccga accgtgccct cggtgatgcc cgcggcgcgc agggcttcgg cgtccatgcg
      901 gaaatgggtg gtgctcgcgg gatggatcac gagcgatttc gcgtcgccga cgttcgcgag
      961 gtgcgagaag acgcgcagcg actcgatgaa gcggcggccg gccgcgcgat cgccctcgag
     1021 gttgaagctg aaaaccgcgc cgcagcctcg aggcagcagg cgcctcgcga gcgcatgatc
     1081 ggggtgcgag ggcagctcgg gatacgcgat gctcgcgacc gccggatggc cggacagaaa
     1141 ggcgatcacc ttgcgcgcgt tctccacgtg gcgctgcatg cgaagcggca gcgtctcgac
     1201 gccttgcagg atctggaacg cggtcgccgg gctcatgcag gcgccgaaat cgcgcaggcc
     1261 ttcgcggcgg gcgcgcagga gaaacgcggc caccgtcgat tcctcggtga agtccatgcc
     1321 gtggaagccg ccgtagggct cggtcagctc gggaaatttc ccggaggcat cccagtcgaa
     1381 gcttcccccg tccacgagca cgccgccgat cgcaacgccg tggccgccga gaaacttcgt
     1441 cgcggagtgg acgacgaggt cggcgccatg gtcgaacgga cgcagcagat aaggcgtcgt
     1501 gaacgtcgag tcgaccagca gcggcacctt ggcggcgtgc gccagctcgg ccaccgcggc
     1561 gacgtcgagc acgtcgagcc ccgggttgcc gagcgtctcg ccgaagaaca gccgggtgct
     1621 cgggcgcacc gcgtcccgcc aggcgccgag atcgcgcgga tcgacgaagg tcgtctcgat
     1681 gccgaagcgc ggcagcgtat aagagagaag gttgtgcgag ccgccgtaga gcgagcgcga
     1741 cgcgacgatg tgcgagccgg cgcccatcag cgtcgcgatc gccaggtgca gggccgcctg
     1801 gccgctcgcc gtcgcgatgg cgccgacgcc tccctcgagc gcggcgatgc gttcctcgag
     1861 caccgcgttc gtcgggttcg agatgcgcga atagacgtgg cccgcgcgct ccatatcgaa
     1921 gagggcggcg gcgtgatcgg cgtcgcggaa gacgaacgag gtcgtgaagt agatcggcgt
     1981 ggcgcgcgcg cccgtgagcg ggtccggctg ctggcctgcg tgcagggtga gcgtgtcgaa
     2041 ctgcaggggg cgcggagcgg ccatggcgtt ctcctcggag tgccgggccg attctagcag
     2101 cggcgcgagc cgcccaaaaa aagaccgcgc ccggaggcgc ggtcttcttc gtcgcagact
     2161 gcccgcgtca gccgagcggc acgggcacgt acacccgcgc gttctcccgt tgcacgagca
     2221 gcgcgacgtt gcccttcgcg cggtcgacca gctcccgcag ctgatcgatg ttcttcaccg
     2281 gctggttgtt cacgccgaga atcacgtcgc ccggctggat cccggccttg gcggcgggcc
     2341 cggtggcgtt ctcgacgatc acgcccgatt cggtgccaag ctgcttgcgc tcctcgccgg
     2401 tcagcggacg caccgccacg ccgagcttgc cctgcggctt ggcttgctct ttctcttgcg
     2461 cgtcggcctt cgccaccttc tcctcgtcgg gcgcgcgccc cacctggacg cgcagatcgt
     2521 gcggctgacc ctggcgcatg atcgacagcg tgatgctcgt cccgggcttg gtctccccga
     2581 tcacccgcgg gaggtcgctc gactgctcga cgggcttgtc gttgaccctg gtgatgacgt
     2641 cgcccgcctg gaaccccgcc ttggcggccg ggctgccggg ctcgaccgag ctgaccagcg
     2701 cgccgtcggt ctgcttgatg ccgaaggcct gcgcgagctc cttggtcatg ggctgcacgg
     2761 tgacgccgag gcggccgcgc tcgacgcggc cgtacttctg gaggtcctcc ttgaccttca
     2821 tcgcgaggtc gatgggcacg gcgaacgaca gtcccatgta gccgccggtg cggctgtaga
     2881 tctgggaatt gatgccgatc acctctccgt tgaggttgaa caggggcccg cccgagttgc
     2941 cgggattgat cgcgacgtcg gtctgcagga acggcacgta gctctcgtcg gggagcgagc
     3001 gcgccttcgc cgagatgatg ccggcggtca ccgagttgtc gaggccgaac ggcgagccga
     3061 tggccgccac ccactcgccg actttcgcct tcgacacgtc gccgatgcgc accacgggca
     3121 ggtcgctggc ttcgatcttg accaggccca cgtcgctgcg cttgtcgacg ccgatgacct
     3181 tggccttgaa ctcacgcttg tccgtgagct tcacggtcac ctgcgtcgcg tccgcgacca
     3241 catgcgcgtt ggtcaggatg tagccgtcgt ggctcacgat gaacccggat cccaggccct
     3301 gccgaacgga attaccctcg ggaatcggga tgttgaagcg cttgaagaac tcgtacagcg
     3361 ggtcgttcgg gtccattccc tgcagccggt cctgcgcgct caccttctgc gtggtgctga
     3421 tgttcacgac cgccggtccg tactgctcga ccagggtgct gaaatccggc agcggccggg
     3481 tcgcctggct gaccgggggc tgctgcgttt gctgcgcttc ctgcttttgc tgcgcggtcg
     3541 ctgcaggcga cgccgtgatc ggcgccagcc ggccgcccgc gtagccgagg agcgtggccg
     3601 cgccgagaca ggcggcaacg acgatcggca gcttgctgtt caccatgcga gtctccttct
     3661 tggggtcctt ccttgggcac tgcacggccc ggcgagttcc gtgcgaaacg ggcagggata
     3721 cagaaggtta cagcggcgtt acggcgcgtc ccgcatgggc cgcgggaagg cgcggagaat
     3781 caggtcgccg cagcctgccg cgcgcgcagg gcttcctcgc gggcttgctg ctccgcgcgg
     3841 gcctgcttga ggatgttggc gcactccggg aagccgccgc tcagcgcgag ctcgatcgcc
     3901 gtgtcgccgc tcttgctgac gaggctgacg tcggcgccgt tgcgcagcag cagctcgacc
     3961 atcttcgcgt ccccgcgcaa ggttgcatac atgagcggcg tgtagccgtt ctcggcgcgc
     4021 aggttggggt cgaaattctt cgacagcagc ttctgggcgt agaccaggtt gcccttctcg
     4081 atcgcgtaga agatggcctt gataccctcg acgctcctct ccttgggctt ggtctccagg
     4141 ccggtcggaa ggatcccgac gacgtagttg atgagcgaga tgcccaccat cgcgatcatc
     4201 gcgatcacga ggggcgcggt cgcgacgccg agcttgtccg cgacgtcggc gggcatgttg
     4261 gccgcgaccg cgagcaggac caccaggaag aagaactgga atttcaggta gagaaacagc
     4321 gcgatgaaga cgatcagcgc gagcagcgcg agcaggacgt tgcgctcgac gctgagggcc
     4381 gcgagccagt cctccggcag gttggcgagc agcgccgtcc cgccgaccag cacgaacagc
     4441 gtccactcgc ggatgttctt gatcttgccc atcgatgctc cctgggccgg gcgtccgggc
     4501 actgtcccgt ctccgacccc ctctttgcgc ctattataat tgtcctgttc ccgccgggcg
     4561 ccctattggt tcatgagcaa agcttttacc cgcgaaaacg acgacgcgct cgaggatgag
     4621 ccggagctag aggcgcagca gccgaagggc gtgaagaact acatcacgcc cgccgggcac
     4681 gcgcgcctga aatccgagct gaagacgctg gtcgaggtcg agcgccccga gctggtcaag
     4741 accgtcgcct gggcggcctc gaacggcgac cgctccgaga acgccgacta catctacggc
     4801 aagcgccgcc tgcgcgagat cgagcggcgg atccgctacc tgatgaagcg cctcgagatc
     4861 gccgaagtgg tggacccgcg cgaccaggac caggaccgcg tgttcttcgg ggcgacggtg
     4921 gtctatcgcg acggcaaggg ccaggagcag acggtgagca tcgtggggac cgacgaggtg
     4981 gaccccgcgc accgccgcgt ctcctggatc tcgccgatcg ccaaggcgct gctgaaggcg
     5041 cgcgaagggg acgccgtgac cgtcaacacg ccgtccggag ccgaggagct cgagatcctc
     5101 gaggtccgct acgacgagtt gcgctagcgc tcagttcgct ggcgcgggct cgctcgctcc
     5161 cgcgaccttc gggaaatcgc ccgccttcac gaccgagagc ttcgccggat cgacatagcg
     5221 ccgcagcgcg gcctggactt cggcgggagt gaggccggca atgcgccgct cgagctgcgc
     5281 gtcccagtcg aaggtcctgc cgacgaacag gtaggaagcg agacggctcg cgagcgcgtc
     5341 gtcctgcgtg cgcgcgacct tgcgcgcctg cagcaggccg cgcttcgcat tctcgacctc
     5401 ctccgcgccg aacccctggt cgagcgcctt gcgcagctcc tccatgatca gcgcctcgac
     5461 acgcgcacgg ttctgcggcg catagatcgc gtacacgccg aacgcgccgt gctggtccag
     5521 gctgtcggcc gagacgtacg agccgacgct gtacgacagg ccctcctgct cgcgcacgcg
     5581 acgcagcagc cgcgaatcgg acccgccgcc gagcaggtaa ttgcccagca gcagcgcggg
     5641 gaagtcggga ttgtcgtcgc ggatcttgag gttcatcccg gcgcggtaga ccgcgttcgc
     5701 cttgtcgggc gtgaggatcg cctcgttgat cgccgacacg tcccggtagg ggtgcgcgat
     5761 tcgcgagtag gggcccgggc ttttccagtc gccgaggagc tgacccagga gccgcgtcac
     5821 ctcgtcggga tcgaagtcgc cgacgaccgc gatctcgccg ttcgatgcgc cgaccaggtc
     5881 ggtgtagcag cgcttcacgt cctcgaggct caggtccttg agcttcgcgg cgcgctcctc
     5941 gagcgtcatg ttgtagagcc agtggccggg cgggtaggga ttcaggtggc gcgagatcgc
     6001 gagcccggcg agcgcgcccg ggtcgctgcg ctgcgtgtcg atgctggtca gcgcggcgcg
     6061 ccggaactgc tcgaactcgc tcgccggaaa ggacggctcg cgcagcacct cggcgacctg
     6121 gcgcagcacg tcgggcaggc tttcgcgcac ggtctcgatc gacccgcctt ccgcgccgat
     6181 gccgacgttc gccttcagcc ggtcgaactc gttgcggatc tgctcgcgcg tcttcttctg
     6241 cgtgccgcgc accagcatcg cgctcgccgc gccgcacgcg gcgctgcgtc cgaccttgct
     6301 ctgctcggtg ccccagcgca ggctgatctg cgccacgacc gtgtggccgc gcgtgcgctt
     6361 cggcagcagg gccagcttga gccctccggg cagcgttccg cggatggtgc gggcctcgat
     6421 attcgccggc gtcggatcga acgcctcgcc ctggacgacc gcgcccgagc ccctgtagtc
     6481 cttcagcgcc gcggcgacgt cgggcaccgc cgggatctcg gcgcggtcga gctccttggt
     6541 cgggatgaac aggcccacgg tgcggttcga cgccttcagg taatgcgcgg cgacgcgctg
     6601 cacgtcctcg ggcgtgacct tgcgcagccg gtcgcggtgc aggtacagca tgcgccagtc
     6661 gccgatcgcg gcgtactccg agagcgcgcc cgtgagctgg cgcgagtccg cgatgctcag
     6721 ctcgatgtcg ttgagcaggc gcgtgcgcgc gcgctcgacc tcgtcgtcgg atggccggct
     6781 gcggccgacc tcctcgaggg tggcgagcag cacgtcgcgc gcgtcgtcga gcgagccgac
     6841 ggggggcagc ccggcgccga aatagccgta gccggcctcg cgctgctggc gctcgctgcc
     6901 aaagacgtag ctcgccttgc cggtctcgac cagggccttg tgcagccgtc ccgagggaac
     6961 gtggttgagc atggcgacca ggacgtcgac cgccgcgtag tcggggtgcg tgcccggagg
     7021 caggtggtac agcaccgaca cgagcggcgc gtcgccgacg cgccgcagcg tcaccgtgcg
     7081 ctcgccgtcc tgcgtcggct cgaccgtgta ggtcttgcgc agcgcgcgcg ccggccgggg
     7141 tagcggtccg aaatgcttct gcaccaggcg cagcgcggcc gcctcgtcga agcggcccga
     7201 gacgatcagc acggcgttgt cgggctcgta gaactcgcgg taaaaggcct gcaggcgctc
     7261 gatcggcacg ttctcgatgt cggagcgcgc gccgatgatc gcacggccgt agttgtgcca
     7321 tacgtaggcc gccgaggcga cgcgttcgcg cagcaccgcg taggggttgt tctcgccgga
     7381 ctcgaactcg ttgcgcacga ccgtcatctc cgcgtcgagg tccttgcgcg acacgcgcgc
     7441 gttgcgcatg cggtcggcct cgatgtcgag cgcccactcg aggttcttgt cgtcggcggt
     7501 gaaggtctcg aaatagttgg tgcggtcgta cgaggtcgtg ccgttgaagc gcgcgccgtg
     7561 cttcaggaac tcgcccttca cgttcggata gcgcggcgtg ccccggaaca gcatgtgctc
     7621 gagcaggtgc gccatcccgg cctcgccgta gccctcgttg cgcgagccca cgaggtaggt
     7681 gatgtgcacc gtgatggtgt cgatcgaggg atccgggacg agaagcagct tcagcccgtt
     7741 ggcgaggcgg tattccgtga cgccttcgat cgaaacgact ttcgcaggcg cgggcgacac
     7801 ccgggacgga gcggccgccc cggcgggggc gggccccgcc ggggcggcca ctgcgggcaa
     7861 caggccgagg agcagcgcgg cgagcgaagc gagcttcacg agcataaggc gaatgggggg
     7921 gaggtgtccg gaatcccgat tgtagcgtca atggcgctgc catcggagcg tcccggccgg
     7981 gcagggttga aatttgcggc gccgggcctc attctggcct ggcgcgggtg gattctttac
     8041 aacctgttac atgatccggc cgcgggcaac cccatggagc acggcatgac gacgacgacc
     8101 gccaaggaga cgctcggctt ccaggccgag gtgaagcagc tcctcgacct gatgatccac
     8161 tccttgtaca gcaacaagga gatcttcctg cgcgagctga tctcgaacgc gtccgacgcc
     8221 gccgacaagc tgcgcttcga ggcgctgtcg gacaatgcgc tgtacggctc ggacgccgat
     8281 ctccggatcc gcgtgtcctt cgacaagtcg gcgcgcacga tcaccgtctc cgacaacggc
     8341 atcggcatgt cgcgcgacga ggtgatccgg aacatcggca cgatcgcgaa gtcgggcacg
     8401 cgcgagttct tccagtccct gacgggggac caggcgaagg acgcgcgtct catcgggcag
     8461 ttcggcgtcg gcttctactc gtcgttcatc gtcgcggacc gcgtcacgct ggtcacgcgc
     8521 cgggcgggcg ccccggccgg cgaggccgtg cgctgggaat ccgggggcgc cggggagtac
     8581 acgatcgaga gcgtcgagaa ggagcggcgc ggcacggagg tcgtgctgca cctgcgcgag
     8641 ggcgaggacg agctgctctc ggacctgcgg ctgaagacca tcatccgcaa gtactccgat
     8701 cacatcgcca ttccgatcgt gatggagaag acgcgctggg acccggagaa gaacgattac
     8761 gtcgcgaccg gcgaggacga gaccgtcaac caggcgagcg ccctgtgggc gcgcccgaag
     8821 agcgaggtca ccgacgagca gtacgtcgag ttctacacgc acgtggcgca cgatttcgag
     8881 gcgccgctcg cctggacgca caaccgggtc gaggggcgcc aggagtacac gcagcttctc
     8941 tacgttccgg cgcgcgcgcc gttcgacctg tgggaccgcc accagcgtcg cggcctgaag
     9001 ctgtatgtgc gccgcgtctt catcatggac gacgccgagc agctgctgcc ggcgtacctg
     9061 cgcttcgtgc gcggcgtggt cgactccaac gacctgccgc tcaacgtctc gcgcgagatc
     9121 ctgcaggaga gcaaggacat cgacgccatc cgctcgggct gcaccaagcg cgtgctcggg
     9181 ctgctcgagg acctggccga gaaccggaaa gatcagtaca cggcgttctg gaaggagttc
     9241 ggccgcgtcc tgaaggaagg cgtcggcgag gacgcggtga acaaggagcg gctggcgaag
     9301 ctgctgcgat tcgcttcgac gcactcggac tccgaggagc aatccgtgtc gctctccgat
     9361 tacgtcgcgc gcatgaagga ggggcagggc ggcatctatt acgtcaccgc cgacagcttc
     9421 ctcgccgcga agaacagccc gcacctcgag atattccgca agcgcggcat cgaggtgctc
     9481 ctgctgcacg agcgcgtgga cgaatggctc gtggccaacc tgcccgagca cgaaggcaag
     9541 ccgttcgtct cggtcgcgcg cggcgagctc gacctgggtc ggatccgcac cgacgaggaa
     9601 aagaaggaag aggagcgcct cgccggcgac ctgcgcgagg tagtcgagaa gctcaaggcc
     9661 gcgctcggcg accgggtgaa ggacgtgcgc gtcacgtcgc gcctcaccgg gtcgccctcg
     9721 tgcctggtcg cggacgagca cgacctcggc ggcaacctcg gccgcatcct caaggccgcg
     9781 ggacagaagg tgccgacggc gaggcccatt ctcgagatca acccgggaca tgcgatcgtc
     9841 cagcggctga agcaggagca gaaccgcttc ggcgactggg cgcacgtgct gttcgaccag
     9901 gcgctgctcg ccgagggcgg ccagctcgac gatcccgcgg gcttcgtcag gcgctcgaac
     9961 gagctcatgc tcgagctggc gggctcgggg ccgaagctct ggacgcccgg tagctgaccc
    10021 cgtatcgtac cgggagcaat gacgcccggt acccgctggc gcggcgcgct cgccgcgttg
    10081 ctcgttttcg ttccgctcgc gctcgcggcg cagcccgcgg ccgatcgtcc gcccgagccc
    10141 gataccggct tccagccg
//
LOCUS       DATDMW010000087        25283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_771195, whole genome shotgun sequence.
ACCESSION   DATDMW010000087 DATDMW010000000
VERSION     DATDMW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25283
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771195"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /gene="purF"
                     /locus_tag="VI319_03625"
     CDS             <1..1359
                     /gene="purF"
                     /locus_tag="VI319_03625"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY6862965.1"
                     /translation="DVFRTRNMRDLVGNWGIGHCRYPTAGSAFDAAEAQPFYVNSPFG
                     LTLAHNGNLTNAEQLKQDMFLQDLRHMNTNSDSEVLLNVLAHELELAAKGQKLDPETI
                     FAAVANVHRRVRGAYAVVAMIAGYGVLAFRDPFGIRPLVFGKAETDRGSEYLVASESV
                     ALTAFGFELVRDVAPGEAIFIDEGGELHSRQCAAKPQLHPCIFEYVYLARPDSVMDGV
                     SVYESRLHMGRQLAAKIKRTMPDLAVDVVIPVPDTSRPSAMELAHGLDVTYREGFIRN
                     RYIGRTFIMPGQAIRKKSVRQKLSVTGLEFQGKNVLIVDDSIVRGTTSREIVQMAREA
                     GANKVYFASAAPPVRFPNVYGIDMPTRSELIATGRTEEEIAREIGADAVVYQDLDALK
                     AAVRAVNPKLANFECSCFDGIYVTGDITPEYLASVERERHAGRQENEDESEQGQLDLN
                     TA"
     gene            1523..2692
                     /locus_tag="VI319_03630"
     CDS             1523..2692
                     /locus_tag="VI319_03630"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEY6862966.1"
                     /translation="MSDYDIETLAVRSGIHRSQFNEHSEALYLTSSFVFENAAQAADR
                     FCNNAPGNIYSRFTNPTVTAFQERLAALEGAGACVATASGMSAILATAMATLKAGDHV
                     VCSSGVFGATVQMLNNILAKFGVETTYADGNDPKSWRSAMKPGTKLVFLESPSNPMTE
                     VFDIAALARVAHDGGALLVVDNCFCTPALQKPIELGADLVIHSATKYLDGQGRVLGGA
                     ILGAKGPVMDAVFSFLRTAGPSLSPFNAWVLLKGLETLKIRMEAQSANALELAKWLES
                     HPKVERVYYPGLASHPQHALAMRQQRSGGAIVSFAVKGGREAAWRVIDSTKLISITAN
                     LGDTKTTITHPATTTHGRITPEARARAGIGEGLMRVAVGLESVRDLREDLARGLG"
     gene            complement(2689..3411)
                     /locus_tag="VI319_03635"
     CDS             complement(2689..3411)
                     /locus_tag="VI319_03635"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY6862967.1"
                     /translation="MAVLFISDLHLSETRPEINRVFFDFLRGRAARAGELWILGDLFE
                     YWAGDDDADDPFNRGVLDAIAQCSRAGARIRVMHGNRDFLMGPEFEAATGAKLVDDPY
                     TAFIGGRTTLLTHGDTLCTDDKEYQAFRRQVRAKDWQERFLALSLSERKKQIAALRER
                     SETEKARKPAEIMDVNPKAVESLLRAHAYPRLIHGHTHRPARHEHVVDGRTCERWVLP
                     DWYETGGVLVCDEGGCRLERPA"
     gene            complement(3419..3910)
                     /locus_tag="VI319_03640"
     CDS             complement(3419..3910)
                     /locus_tag="VI319_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6862968.1"
                     /translation="MVRFHTNHGVIGIELDDKRAPETVKNFLGYVESGFYANTVFHRV
                     IDGFMIQGGGFEPGMRQKPTRAPIQNEAANGLKNDRYSIAMARTNDPHSASSQFFINV
                     KDNSFLNHSAPSAEGWGYCVFGKVVEGQDVVDKIKGVKTGKRGFHSDVPVEDVVIEKA
                     EVV"
     gene            complement(3919..4467)
                     /locus_tag="VI319_03645"
     CDS             complement(3919..4467)
                     /locus_tag="VI319_03645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010808965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6862969.1"
                     /translation="MLFAAAAWAQNPRVEMKTSAGRIVIELDAANAPKTSANFLQYAK
                     DGFYNGTIFHRVIDGFMIQGGGFDRDMRQKPTRAPIANEAGRSSKGGLRNDVGTVAMA
                     RTSDPDSATAQFFINVHRNDFLNFREPTPQGYGYAVFGRVVEGMEVVQRIAKVPTATV
                     GPYQNVPRSPIVIESATVLPAK"
     gene            complement(4536..5783)
                     /locus_tag="VI319_03650"
     CDS             complement(4536..5783)
                     /locus_tag="VI319_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY6862970.1"
                     /translation="MNAKGRIAVAAAAVVAVVVMGALATFGKSVAPAVARAIDPDAAK
                     MMMAGDRTADAYLARTFEDISHERFDLAQGEIDALLKVKPNFRLAHLIKGDLLLARAR
                     PVTAIGAVKNAPSDRLEGLRAEAIARLRAYRERPRADLVPRYLMQMRDDQKYAIVVDT
                     HRSRLYLYENVDGRPRFVADYYVSSGKNGAAKMREGDEKTPVGVYHVVASLPRQKLSD
                     FYGTGAFPIDYPNEWDRLKGRNGHGIWLHGVPSDTYSRPPRASNGCVVLANADLDAIA
                     RDLQVGLTPVIISEDVEWTSFDDWAQERKAFQAQLEQWRADWESRDADRYLGHYSRSF
                     RANGEDLAAWSRHKRQVNTGKTWIKVGVRNVSMFRNPGKEDLVVVTFDQDYRSNNLSN
                     TMRKRQYWEREGGTWRIVYEGSA"
     gene            complement(5783..6790)
                     /locus_tag="VI319_03655"
     CDS             complement(5783..6790)
                     /locus_tag="VI319_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HEY6862971.1"
                     /translation="MNFVLRPAALCAALALAAALAALPTRADDIQDINKLIRAGQHAQ
                     AMDRVNQVLAKKPRDAQARFLKGVIFAEQNKPQEAIDVFAKLSQDYPELPEPYNNLAV
                     LYAAQGQYEKARQQLEMSIRTHPSYATAYENLGDVYAKLASQAYDKALQLDSSNSTAK
                     SKLSLIKELISRAPSATPAVAAQPPAAVAAAPKSAPPAAAEAKSEAAAPKTEPAKATA
                     TPPGAGDEVLKTVQAWASAWSKKDVDAYLAFYANDFKPPKGEARAQWEKARKERISAP
                     KRIQVTIEAPKVTLKGDDNAVVSFRQKYRSDNLQSSNSKTLILVRAEGKWKIQQERVG
                     G"
     gene            6978..8357
                     /gene="cysS"
                     /locus_tag="VI319_03660"
     CDS             6978..8357
                     /gene="cysS"
                     /locus_tag="VI319_03660"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY6862972.1"
                     /translation="MLKIYNTLTRAKEEFTPIAAPRVGMYVCGMTVYDHPHIGNARAM
                     VAFDVAQRWLRACGYEVTYVRNVTDIDDKIIRRAAESGEAPGALAERFRRAMEEDFAA
                     LGIQKPDLEPRATQHVDDMQALIGALERRGLAYQAQDGDVCFPVRKFPGYGRLSGKSL
                     DELRAGERVEVDAGKRDPLDFVLWKRAKAGEPSWRSPWGEGRPGWHIECSAMSMKLLG
                     EHFDIHGGGQDLQFPHHENEIAQSEGALADGRPFVRYWMHNAFVRMDEEKMSKSLGNV
                     VTLPQALARHDGEALRFFFARAHYRSPLSYTERNVEDARQALATLYTALKTARSEAAA
                     PDWAEPHGARFRAAMDDDFNTPEAVAVLFDLATEVNRSRDAVLARQLRALGGVLGLLG
                     RDASAFLQGAPASEAGISSAEIEGLIAARNAARAAKNYAESDRIRKSLGERGIVLEDG
                     PGGTTWRRG"
     gene            complement(8358..9494)
                     /locus_tag="VI319_03665"
     CDS             complement(8358..9494)
                     /locus_tag="VI319_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862973.1"
                     /translation="MRVSTISLISLFIVAAALPVGAPAQGVTKTTILVGQSGPLSGSN
                     KEFGEDIRDGANAYFRRINDAGGIHGRKIQMVTLDDANDAKKSGENGRILIEERGVVA
                     LFGYASATLSMPALPFVEKHRVPFVAPFTGADPMRKFNPLVYNVRASYADELEKIVEF
                     YTTTGMSKFAVIHYDDTVGKENLAAVQAALARRSLKPVAIGTLQRNQTTLGDAVNGVL
                     AAQPDVVIATTLYRTTADFIKQARRGGSGAQFASTSFVGASALAGELKDQGSGVVVAQ
                     VVPPYSKASVPVVKEYQAAIEKLLGKRAFSFTSLESYISAKVLAEAMRRAGPNLTRET
                     LIKALDSMQNFDAGGYVVDFNPKEHNGSKFVELTAIGKSGRFAY"
     gene            complement(9553..10686)
                     /locus_tag="VI319_03670"
     CDS             complement(9553..10686)
                     /locus_tag="VI319_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6862974.1"
                     /translation="MNIRYLATLAGVCATVGTAFAQTPGVTPTTIVLGQSAAFSGPAA
                     QLGIQMNIGTKAYLDYVNAKGGVHGRKIELKTRDDKYEANLAVENTKKLIEEDKVFAL
                     ISYVGTPTTGAAMPIFTQAKVPLVGPFTGAEVLRTPVNRYIFNVRASYYDETEKIVEQ
                     LVSTGNKNIAVFYQDDAYGQAGLKGVEIAMTKRSLKISALGKVERNTTKVQDAVKTID
                     AAKPDAVIMISAYTSIAEFVREMRAAGSVAQFHNVSFVGSKALSDALKDEGYGVAISQ
                     VVPFPWSPAVPIVKEYQQVLKQAGNTDYNFSSLEGFIVAKVMVEGLKRAGRDLTREKL
                     VAALESMNSVDLGGFNVSFSPSNHSGSQYVDLTMIGRQGKFLK"
     gene            complement(10897..12021)
                     /gene="dnaJ"
                     /locus_tag="VI319_03675"
     CDS             complement(10897..12021)
                     /gene="dnaJ"
                     /locus_tag="VI319_03675"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY6862975.1"
                     /translation="MAKRDYYEVLGVNRDADEETVKKAYRRLAMKYHPDRNPDNPKAE
                     ELFKEAKEAYEVLCDGAKRGAYDRYGHAGVDPQAGMAGAGAAGFGGFADAFGDIFGDI
                     FGQARGGRSSVFRGADLRYNLEVTLEQAAHGTETRIRIPTHEACDVCGGSGAKPGTKP
                     RTCSTCNGHGQVRMQQGFFSLQQTCPNCHGSGKVIPEPCPTCHGAGRVKKQKTLSVRI
                     PEGVDEGDRIRLAGEGEAGVNGGPTGDLYVQIQVKPHPVFQRDHDDLHCEMPISFTTA
                     ALGGDIEIPTLDGSAKIRVPAETQSGKIFRLRGKGIKGVRSREHGDLLCHVMLETPVN
                     LTDRQKELLREFETLSQKDSERHNPRAKSWMAKVKEFFAE"
     gene            complement(12122..14047)
                     /gene="dnaK"
                     /locus_tag="VI319_03680"
     CDS             complement(12122..14047)
                     /gene="dnaK"
                     /locus_tag="VI319_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY6862976.1"
                     /translation="MAKIIGIDLGTTNSCVAIMEGGKPKVIENSEGSRTTPSIVAYLE
                     DGEILVGAPAKRQAVTNPKNTLFAVKRLIGRRFEEKEVQKDIDLMPYKIVKADNADAW
                     VEVRGKKIAPPEVSAQVLRKMKKTAEDYLGEEVSEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGLDKKEGDRKIAVYDLGGGTFDISIIEIAEVEGEHQFEV
                     LSTNGDTFLGGEDFDQRLMDYLCDEFKKEQGIDLRADVLALQRLKEAAEKAKIELSSS
                     QQTEVNLPYITADKNGPKHLAIRVTRAKFESLVEDLISKTVEPCKTALKDAGLKLSEI
                     EDVILVGGMTRMPKVQETVRDLFGKEPRKDVNPDEAVAVGAAVQGGVLKGDVKDVLLL
                     DVTPLSLGIETLGGVMTKLIQKNTTIPTKAQQVFSTAEDGQTAVTIHVLQGERDMSSG
                     NKSLGQFNLTDIPPAPRGMPQIEVTFDIDANGILHVSAKDKATGKENKIKIQASSGLN
                     EAEIQRMVKDAEAHAAEDKKQLEVVTARNQLDALAHSVRKSLKEHGDKIGADEKTKIE
                     AAIKDAEEALKTDDKDRIDAKAQALAQASQKLGEKMYADAQQQQQQAGAEAKGSKKTD
                     DSNVVDAEFTEVKDKKG"
     gene            complement(14137..14694)
                     /gene="grpE"
                     /locus_tag="VI319_03685"
     CDS             complement(14137..14694)
                     /gene="grpE"
                     /locus_tag="VI319_03685"
                     /inference="COORDINATES: protein motif:HMM:NF010737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY6862977.1"
                     /translation="MATNEPQAPIGNADAEMEARETEQKRAEREPEAIPAAEELLRQA
                     ELKAQEHHDAWLRAKAETENVRKRAQVEVANAHKYAIENFAQELLPVKDSLEAALATD
                     TASADAIRSGVELTLKQLAAVFEKANLKEINPVGQKFDPHRHQAISMLPSDREPNTVI
                     NVLQKGYLLNDRVIRPALVTVSQAK"
     gene            complement(14713..15828)
                     /gene="hemH"
                     /locus_tag="VI319_03690"
     CDS             complement(14713..15828)
                     /gene="hemH"
                     /locus_tag="VI319_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY6862978.1"
                     /translation="MRYDPEPERQHGSPAKTGVLLVNLGTPDAPTAASVRRYLAEFLW
                     DPRVVEIPRALWWPILHGVVLRLRPRASARRYASIWTPEGSPLKVHAEKQAGLLRGTL
                     AARIRAPLAVDAAMRYGAPAIAEVLRRLKRDGCDRVLVVPLYPQYAASSTASVLDEVA
                     AFIRRTRNVPALRIVRGFHDHPAYIGALAARVREHWQAAGRPDRLVMSFHGLPQRSVE
                     RGDPYQSECRQTARLLADALGIGEDRWQMTFQSRFGAGEWLKPYTASTLADLGRAGMR
                     RVDVVCPGFVADCLETLEEIGIEGKQTFLAAGGGEFRLVPALNEHEAWIGALASIVLE
                     NLTGWIGPEAEARNLKSPGRAQASIVESGGAEQPRAR"
     gene            complement(15846..16880)
                     /gene="hrcA"
                     /locus_tag="VI319_03695"
     CDS             complement(15846..16880)
                     /gene="hrcA"
                     /locus_tag="VI319_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY6862979.1"
                     /translation="MQLDRRAQVLLKTLVERYIAEGEPVGSRALSRFSGLDLSPATIR
                     NVMADLEDLGFVVSPHTSAGRVPTPRGYRLFVDSLLTIKPLGQIEISQVEVSLNPGQP
                     QNLIASASQLLSELTHFAGVVVASKRKSPAFRHIEFLALSDKRVLLIIVTAEGDVQNR
                     ILFTDRTYTPSELTYAANFLNQNYAGLTFDEIHRRVRDELQRLREDMTALMTAALEAG
                     SQAVSESTEAYAVSGERNLLDSQDLAANMTRLRELFELFEKKALLMQILDLSSRSQGV
                     QIFIGGESGIAPLDECSVVTAPYEVEGQVVGTVGVIGPTRMAYERVIPIVDITAKLLS
                     SALSQHRLEG"
     gene            16962..17837
                     /locus_tag="VI319_03700"
     CDS             16962..17837
                     /locus_tag="VI319_03700"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY6862980.1"
                     /translation="MPAFKTIALIGRHNAPDIGGAVLAMAHFLEREGCTVLIERRTAE
                     SCGLQAPPAHADYTEIGARADLAVVLGGDGSMLTAARSLAAKRVPLVGVNQGRLGFLT
                     DIAAREMTETMEQILAGRYSLEERTMLAAEVRRGDTVAFSTLALNDAVVNKGAVGRLI
                     EFHVHIDGEFVYDLRSDGLIVTTPTGSTAYSLSSNGPILEPRVPAFALVPISPHTLSN
                     RPVAVSDRSVIEITLRHSIDARVHFDGQPQFDLHDGDRVTIRRAAETTTLVHPPGYSY
                     YGMLREKLHWAGGAL"
     gene            17842..19503
                     /gene="recN"
                     /locus_tag="VI319_03705"
     CDS             17842..19503
                     /gene="recN"
                     /locus_tag="VI319_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY6862981.1"
                     /translation="MLTRLSIRDFAIVDHLELDFSRGFTALTGETGAGKSILIDALSL
                     ALGERAGAELVRAGCERAEVTAEFAIDRIAPLQSWLRDQALEDDGGSVLLRRVIDAGG
                     RSRAFINGRAATLNQLREVGEWLVDIHGQHAHQSLLKGEMQRAVLDDHAGLSQLADET
                     AGAFREWQKLCRARAEHQTHAAARNAEREQLSWQIEELRRLAPAAGEWDAIQSEHGRL
                     AHAASLIEGAQGAIDALSESETAALAVLSGVLSRMRPLVDYDAALKDTVGLLESGEAN
                     LREAAYSLRHYVDRIELDPARLREVEARLEALHSAARKFHARPEALPERLAELEARLA
                     ELDTATDLEALAKQEQAARSRYDALAARLSAERRKAAAKLSREITAAMKELAMGGGRF
                     EVELRSLLPDGSTGGNEQVEFLVTANPGVEPRPLAKVASGGELSRVSLAIQVITSKAA
                     RVPTLIFDEVDAGIGGAVAEVVGRKLRSLGEDRQVLVVTHLPQVAAQATEQWSVAKGA
                     GGAGVRSRVTVLDRNSRIEEIARMLGGVAITATTRKHAAEMLGLR"
     gene            19533..19901
                     /locus_tag="VI319_03710"
     CDS             19533..19901
                     /locus_tag="VI319_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862982.1"
                     /translation="MKTRKHSRGVGQIVFLVVLALLLAGGVLFLDRYNAGTRDMLTVE
                     TRGLQMISALTRYKLENGGYPDSLDKLVPKFTPAVSACPSGTPIEYRLSGGNYVLTCE
                     NVVLKMQPYHYDSRTRAWGS"
     gene            19954..20030
                     /locus_tag="VI319_03715"
     tRNA            19954..20030
                     /locus_tag="VI319_03715"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19988..19990,aa:Val,seq:gac)
     gene            20066..20371
                     /locus_tag="VI319_03720"
     CDS             20066..20371
                     /locus_tag="VI319_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862983.1"
                     /translation="MLSACSSTPVWQPSGYKPSSVQLLVTHYLPPGCDNGACAIRHGT
                     SGDCIIYMLPKLEGRKDVLDHELCHCLGYDHGKEVPGLLVVPTCPVDTGRVEYKVSS"
     gene            complement(20384..20947)
                     /locus_tag="VI319_03725"
     CDS             complement(20384..20947)
                     /locus_tag="VI319_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862984.1"
                     /translation="MQRRTRLALLFTALAGVAAWAAFGGKDAQHAPASVASTAKPSPA
                     APQPAAASEIPERATLGQMGADPFNTEPPALPAPVVSAPPPAPAKPVAPPMPYRFAGR
                     LHVGNSVEVYLMKGEELVPVKKGDTLDGQYKIEKIGRTEMTLLHLASRTREKIEYDPP
                     IKDDDAVADAPAPEAHAEAQRRSPSGG"
     gene            complement(21005..21517)
                     /locus_tag="VI319_03730"
     CDS             complement(21005..21517)
                     /locus_tag="VI319_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY6862985.1"
                     /translation="MKRPFGTQRGFTYLTVLFVVAFMSLGMAKVGQVYRTAVLRERES
                     ELLWRGNQYRLAIEAYTKRGGGLYPRSLDDLVRDPRDVTVRRYLRKRYPDPIMGSDEW
                     GLVKAPDGGIMGVYSRSEDQPVKAAGFRLADAAFEGAAKYSDWKFVYQPGAPGAALPV
                     PQASPGRPPG"
     gene            complement(21525..21926)
                     /locus_tag="VI319_03735"
     CDS             complement(21525..21926)
                     /locus_tag="VI319_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6862986.1"
                     /translation="MKIKHLGPSTLLRTGFTLIELLVVLAIVALLLTIAVPRYFGSLD
                     KSKEAVLKEDLFQMRDAIGKYYGDKGKYPDTLDALVSEKYLRSVPVDPITEKKDSWVA
                     VQPADGDATGVSDVKSGADGKTADGTSYADL"
     gene            complement(21931..22407)
                     /locus_tag="VI319_03740"
     CDS             complement(21931..22407)
                     /locus_tag="VI319_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY6862987.1"
                     /translation="MSKRPKGFTLIELMITVAIVALLASAAFPVSELVVRREKESELR
                     RDLRQIRDAIDAYKLAWDEGRVKKSIDETGYPKSLEQLAEGVENEKSPKREKIFFLRR
                     VPRDPFNADASLSAAATWGKRSYASDPDEPRDGADVFDVFSRAPGKGSDGRPYKEW"
     gene            complement(22418..24355)
                     /locus_tag="VI319_03745"
     CDS             complement(22418..24355)
                     /locus_tag="VI319_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY6862988.1"
                     /translation="MMTKASRRCALLLIACLALLSACASIKTDPVVEESRTLLGSGHG
                     EEALARLDQAQREHPEDRTFRTEYFHERDLLIGQWLGQAQALSAASQYTAAGELYRKV
                     LKYDPGNVRAKNGLSQLDVEERNRAVLNEVQDLVKAEKYREAEEKLAPLLSANRNNRE
                     AQRLQRQIEERTAKPAVPQLKVQATKPISLELREVPLRSAFEVIGRAANINFMFDKDV
                     KADQKTTIVVKDARIEEAIRLVLTSNQLDYKVLNETTALVFPNTPQKQRDYQELVVKS
                     FYLANADVKQTATMIRTILKTRDVFVDEKLNMLVMRDTPKAVRLAERLIAAQDIAEPE
                     VMLEVEVLEVGANRLLDLGLKFPDSVSAGVQGAAGVPGQISLPEYLNRNSGLVRLNFS
                     DPLFLLNLRQQDGRTNLLANPRIRVKNKEKARIHIGDRVPVITTTAAVAGGFVSESVS
                     YLDVGLKLDVEPIVYLEDDVGINIGLEVSNIVNTVRGSTGNTLAYQIGTRNATTVLRL
                     HDGETQVLAGLINDEDRRTASRVPGLGDMPVLGKLFSQTSDTRNKTEIVLLLTPHIVR
                     TIERPGPRTTEFSAGTETSVEGPAVVGTGPSPMIVEQPPVQPPQRPAPAPTPAPAPAP
                     PGNTMVPFGGVQQQPYAAPKQ"
     gene            complement(24352..24900)
                     /locus_tag="VI319_03750"
     CDS             complement(24352..24900)
                     /locus_tag="VI319_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862989.1"
                     /translation="MKIGVKSLRNSLRRLGVPGVLGAGVLVACLPFYFSSVRPAEREL
                     QARRLAAEQTRARAGIQTVSAPRGPELGRFYQAFPAMDRLPDELERLYGIARASRLDL
                     SQGEYRLEQRGPGLASYRIALPLRGSYPDIRGFLSDTLQQMPYAAVDAVRFERKKVGD
                     TQLDAQVRLTLFFRPSNRNEIR"
     gene            complement(24897..>25283)
                     /locus_tag="VI319_03755"
     CDS             complement(24897..>25283)
                     /locus_tag="VI319_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862990.1"
                     /translation="RDIAALAAVQGALDLTQTREPAKTNDEELKQAETVVRQLTLPWS
                     DIVRAIEGASMRDVGVLNMQPDAQQRLLKLTAEAKTREAMLEYVRRIAQTQGLSNVYI
                     VSHQVQTDNPSRPIQFSVQASLGAPK"
BASE COUNT         3608 a         8528 c         8685 g         4462 t
ORIGIN      
        1 gacgtgttcc gcacgcgcaa catgcgcgat ctcgtcggca actggggcat cggccattgc
       61 cgctatccga cggcgggctc cgccttcgac gccgccgagg cgcagccgtt ctacgtcaac
      121 tcgccgttcg ggctgacgct ggcgcacaac ggcaacctga ccaacgccga gcagctcaag
      181 caggacatgt tcctgcagga cctgcgccac atgaacacca actcggactc cgaggtgctg
      241 ctcaacgtgc tcgcgcacga gctcgagctc gccgccaagg gccagaagct cgatcccgag
      301 acgatcttcg ccgcggtcgc gaacgtgcac cgccgcgtgc gcggcgccta cgccgtcgtg
      361 gcgatgatcg cgggctacgg cgtgctcgcg ttccgcgatc cgttcgggat ccggccgctc
      421 gtgttcggca aggccgagac ggaccgcggc agcgagtacc tggtcgcctc ggagagcgtg
      481 gcactcacgg cgttcgggtt cgagctcgtg cgcgacgtgg ctccgggcga ggcgatcttc
      541 atcgacgagg gcggagagct ccacagccgg caatgcgcgg cgaagccgca gctccatccc
      601 tgcatcttcg agtacgtgta cctggcgcgc cccgattccg tgatggacgg cgtgtccgtc
      661 tacgagagca ggctgcacat ggggcggcag ctcgccgcca agatcaagcg cacgatgccc
      721 gacctggcgg tcgacgtcgt gatcccggtt cccgacacga gccgtccaag cgcgatggag
      781 ctcgcgcacg ggctcgacgt cacgtaccgc gagggcttca tccgcaaccg ctacatcggg
      841 cgcaccttca tcatgccggg ccaggcgatc cgcaagaagt cggtgcggca gaagctctcg
      901 gtcacgggcc tggaattcca gggcaagaac gtgctcatcg tcgacgattc catcgtgcgc
      961 ggcacgacca gccgggagat cgtgcagatg gcgcgcgagg ccggggcgaa caaggtctat
     1021 ttcgcgtccg ccgcgccgcc cgtgcgcttc ccgaacgtct acggcatcga catgccgacg
     1081 cgctccgagc tgatcgccac gggccgcacc gaggaggaga tcgcgcgcga gatcggagcc
     1141 gacgcggtgg tctaccagga cctcgacgcg ctgaaggccg cggtgcgcgc ggtgaatccg
     1201 aagctcgcga acttcgagtg ctcgtgcttc gacggcatct acgtcacggg cgacatcacg
     1261 ccggagtacc tcgcgtcggt cgagcgcgag cgccacgcgg gacgccagga gaacgaggac
     1321 gagagcgagc agggccagct ggatctcaac accgcctgag ccggtgaatt tgacattccc
     1381 cttgccgcag agtaacttgc gtactgcgcc gatttgatcg acagcttgcg ggcgctctat
     1441 aaatacggct aaagcgcggc gaagcccgtc ttagcgaaag ctggacgggt tttttcattc
     1501 tgcctatcga aactgagtgc aaatgagcga ctacgacatc gagacgctgg cggtgcgctc
     1561 cggcatccac cgcagccagt tcaacgagca ttccgaggcg ctgtatctca cctcgagctt
     1621 cgtgttcgag aacgcggcgc aggccgccga ccgcttctgc aacaacgcgc cggggaacat
     1681 ctactcgcgc ttcaccaacc cgacggtgac cgcgttccag gagcgcctcg ccgcgctcga
     1741 gggcgccggg gcgtgcgtgg cgaccgcgtc gggcatgtcg gcgatcctcg cgacggcgat
     1801 ggccacgctg aaggctggag atcacgtcgt gtgctcctcg ggcgtgttcg gcgcgacggt
     1861 gcagatgctc aacaacatcc tcgccaagtt cggcgtcgag acgacctacg ccgacggcaa
     1921 cgatccgaag agctggcgct cggcgatgaa gccgggcacg aagctggtgt tcctcgagtc
     1981 gccgtccaac ccgatgacgg aggtgttcga catcgccgcg ctcgccaggg tcgcgcacga
     2041 cggcggtgcg ctgctggtgg tggacaactg cttttgcacg cccgcgctgc agaaaccgat
     2101 cgagctgggc gccgacctcg tgatccactc cgcgaccaag tacctcgacg ggcaggggcg
     2161 ggtgcttggc ggcgcgatcc tcggcgccaa aggaccggtg atggacgccg tgttctcgtt
     2221 cctgcgaacc gcggggccca gcctgtcccc gttcaacgcg tgggtgctgt tgaaggggct
     2281 cgagacgctc aagatccgga tggaggccca gtcggccaat gccctcgagc tggcgaagtg
     2341 gctcgagtcg cacccgaagg tcgagcgcgt gtactacccg ggtctcgcgt cgcatccgca
     2401 gcacgcgctc gcgatgcgcc agcagcgctc gggcggcgcg atcgtctcct tcgccgtgaa
     2461 gggcgggcgc gaagccgcgt ggcgggtgat cgactcgacg aagctgatct cgatcaccgc
     2521 gaacctcggc gataccaaga cgaccatcac gcatcctgcg acgacgacgc acgggcggat
     2581 cacgcccgag gcgcgggcgc gggccggaat cggggagggg ctgatgcggg ttgcggtcgg
     2641 tctcgaatcc gtccgcgatc tgcgcgagga tctcgcgcgc gggctgggct aggcaggccg
     2701 ctccaggcgg cagcctccct cgtcgcacac gagcacgccg cccgtctcgt accagtcggg
     2761 gagcacccag cgctcgcagg tgcgcccgtc gacgacgtgc tcgtgacgcg caggccggtg
     2821 cgtgtggccg tggatcagcc gcggataggc gtgcgcgcgc agcagcgact cgaccgcctt
     2881 cgggttcacg tccatgatct cggcgggctt gcgcgccttc tcggtctcgc tgcgctcgcg
     2941 caaggccgcg atctgcttct tgcgctctga gagggacagg gcgaggaagc gctcctgcca
     3001 gtctttcgcg cgcacctgcc ggcggaacgc ctggtattcc ttgtcgtcgg tgcagagcgt
     3061 gtcgccgtgg gtgagcagcg tcgtgcggcc gccgatgaac gcggtgtaag ggtcgtcgac
     3121 gagcttggcg ccggtcgccg cctcgaactc cggtcccatg agaaagtcgc ggttgccgtg
     3181 catgacgcgg atgcgggcgc ccgcgcgaga acattgcgca atcgcgtcga gcacgccgcg
     3241 gttgaacggg tcgtcggcgt cgtcgtcccc ggcccagtac tcgaagaggt cgccgaggat
     3301 ccagagctcg ccggcgcgcg cggcgcgacc gcgcaggaag tcgaagaaga cccggttgat
     3361 ctccggccgg gtctccgaga gatgcagatc cgagatgaac aggaccgcca tccggatcct
     3421 agacgacctc cgccttctcg atgacgacgt cctcgaccgg cacgtcgctg tggaagccgc
     3481 gcttgcccgt cttcacgccc ttgatcttgt cgacgacgtc ctggccctcc acgaccttgc
     3541 cgaacacgca gtagccccag ccttcggcac tgggcgcgct gtggttcagg aacgagttgt
     3601 ccttcacgtt gatgaagaac tggctcgacg ccgagtgcgg gtcgttcgtg cgcgccatgg
     3661 cgatggaata gcggtcgttc ttcaggccgt tcgccgcctc gttctggatc ggcgctcgcg
     3721 tcggcttctg gcgcatcccc ggctcgaagc cgccgccctg gatcatgaag ccgtcgatca
     3781 cccggtggaa cacggtgttc gcgtagaacc cggattcgac gtagccgagg aagttcttga
     3841 ccgtctcggg agcgcgcttg tcgtcgagct cgatgccgat gacgccgtgg ttggtgtgaa
     3901 acctgaccat gatgcctcct acttcgcggg cagcacggtc gccgactcga tcacgatcgg
     3961 cgaccgcggt acgttctggt acggcccgac ggtggcggtc gggaccttgg cgatcctctg
     4021 tactacctcc atgccctcga cgacgcggcc gaataccgcg tacccgtagc cctgcggggt
     4081 gggctccctg aagttcagga aatcgttcct gtggacgttg atgaagaact gcgcggtggc
     4141 cgagtcgggg tccgacgtgc gcgccatcgc gacggtgccg acgtcgttgc gcaggccccc
     4201 cttggaggag cgcccggcct cgttggcgat cggtgcgcgc gtgggcttct ggcgcatgtc
     4261 gcggtcgaag ccgccgccct ggatcatgaa gccgtcgatc acgcggtgga agatcgtgcc
     4321 gttgtagaag ccgtccttcg cgtactgcag gaagttggcc gacgtcttcg gcgcgttcgc
     4381 ggcgtcgagc tcgatcacga tgcggccggc gctggtcttc atctccacgc gcgggttctg
     4441 cgcccacgcg gcggcggcga agagcatcag cgaagcgagc acgagattgc gcatggccac
     4501 tgtccttcga tggggtgatg cgggaagcga aaggctcagg ccgagccctc gtagacgatg
     4561 cgccaggtgc caccctcgcg ctcccagtac tggcgctttc tcatcgtgtt cgacaagttg
     4621 ttcgagcggt aatcctggtc gaaggtcacg accaccaggt cttccttgcc cggattgcgg
     4681 aacatgctga cgttgcgcac gcccaccttg atccaggtct tgccggtgtt gacctggcgc
     4741 ttgtggcggc tccaggcggc gaggtcctcg ccgttcgcgc ggaaactgcg cgagtagtgg
     4801 ccgaggtagc gatcggcgtc gcggctctcc cagtcggcgc gccactgctc gagctgcgcc
     4861 tggaacgcct tgcgctcctg cgcccagtcg tcgaagctgg tccactcgac gtcttcgctg
     4921 atgatcaccg gcgtgagacc cacctgcagg tcgcgagcga tcgcgtcgag gtcggcgttg
     4981 gcgagcacca cgcagccgtt gctcgcgcgc ggcggacggc tgtaggtgtc gctcggcacg
     5041 ccgtgcagcc agatgccgtg gccgttgcgg cccttgagcc ggtcccactc gttgggatag
     5101 tcgatcggaa acgcgccggt gccgtagaaa tcggacagct tctgccgcgg caaactggcg
     5161 acgacgtggt agacgcccac cggcgtcttc tcgtcgccct cgcgcatctt cgccgcgccg
     5221 ttcttccccg agctgacgta gtagtcggcg acgaagcgcg ggcggccgtc gacgttctcg
     5281 tacaggtaca ggcgcgaccg gtgcgtgtcg acgacgatcg cgtacttctg gtcgtcgcgc
     5341 atctgcatca ggtagcgcgg caccaggtcc gcgcgcggcc gctcgcggta cgcgcgcagg
     5401 cgtgcgatcg cctcggcgcg caggccctcc agacggtcgc tcggcgcatt cttcaccgcg
     5461 ccgatggcgg tcacgggacg cgcccgggcg agcagcaggt cgcccttgat caggtgcgcc
     5521 aggcggaagt tcggcttgac cttgagcagc gcgtcgatct cgccctgcgc caggtcgaag
     5581 cgctcgtgcg agatgtcctc gaacgtgcgc gcgaggtagg cgtcggcggt gcggtcgccg
     5641 gccatcatca tcttggcggc gtcggggtcg atcgcccggg cgacggcggg cgcgacgctc
     5701 ttgccgaagg tggcgagcgc gcccatgacg acgaccgcca cgaccgcggc cgcggcgacg
     5761 gcgatgcgtc ccttggcgtt catcagcccc cgacccgttc ctgctggatc ttccacttgc
     5821 cctcggcgcg cacgaggatc agcgtcttgc tgttgctcga ctgcaggttg tccgagcggt
     5881 acttctggcg gaacgagacc accgcgttgt cgtcgccttt cagggtcacc ttgggcgcct
     5941 cgatggtcac ctggatgcgc ttcggcgcgg agatccgctc tttgcgcgcc ttctcccatt
     6001 gtgcgcgcgc ctcgcccttg gggggcttga agtcgttggc gtagaacgcg aggtaggcgt
     6061 cgacgtcctt cttcgaccac gcgctcgccc aggcctgcac cgtcttcagc acctcgtcgc
     6121 cggctccggg cggggtcgcg gtcgccttcg cgggctcggt cttgggcgca gcggcctcgc
     6181 tcttggcctc ggcggcggcg ggcggcgcgg acttcggcgc tgcggcgacc gcggcgggcg
     6241 gctgcgcggc cacggccggc gtcgcggacg gcgcgcggct gatcagctcc ttgatcagcg
     6301 acagcttgct cttcgccgtc gagttggagg aatcgagctg gagcgccttg tcgtacgcct
     6361 ggctcgcgag cttggcgtag acgtcgccga ggttctcgta agccgtcgcg tagctcgggt
     6421 gcgtgcggat ggacatctcg agctgctgcc gtgctttctc gtactggccc tgggccgcat
     6481 acagtaccgc gagattgttg tagggttccg gcagctcggg gtagtcctgt gagagcttgg
     6541 cgaagacatc gatcgcttcc tggggcttgt tctgctcggc gaagatcacg cctttcagga
     6601 agcgcgcctg cgcgtcgcgc ggcttcttgg cgagcacctg gttcacgcgg tccatcgcct
     6661 gcgcgtgctg gccggcgcgg atcagcttgt tgatgtcctg gatgtcgtcg gcgcgcgtcg
     6721 ggagggcggc gagcgcggcc gccagcgcaa gcgcggcgca gagggcggcg ggccgaagga
     6781 cgaagttcat tgtgctatgc tttcctcgcc tgacggacaa cgcgtcattc tagcaagaaa
     6841 gcccgcgaaa tccacaataa tcagcccttt gccggaattt cttcgcctgt tcccttcgcc
     6901 gcggacgagc ccctggaagg ccgcgcactg atccttccgg caccgccgat tccccaccct
     6961 tcgacccgcc gcggcccgtg ctgaagatct acaacaccct gacgcgggcc aaggaggaat
     7021 tcactccgat cgcggcgccc cgcgtcggca tgtacgtgtg cgggatgacc gtctacgacc
     7081 acccgcacat cggcaacgcc cgggcgatgg tcgcgttcga cgtcgcgcag cgctggctgc
     7141 gcgcctgcgg ctacgaggtc acgtacgtgc gcaacgtcac cgacatcgac gacaagatca
     7201 tccgccgcgc ggccgagagc ggtgaggcgc ccggcgcgct cgccgagcgc ttccggcgcg
     7261 cgatggagga agacttcgcc gcgctcggca tccagaagcc cgatctcgag ccgcgcgcga
     7321 cgcagcacgt cgacgacatg caggcgctga tcggtgcgct cgagcggcgc gggctcgcgt
     7381 accaggcgca ggacggcgac gtctgcttcc cggtgcgcaa gttccccggc tacggcaggc
     7441 tgtcggggaa gtcgctcgac gagctgcgcg ctggagagcg cgtcgaggtc gacgccggga
     7501 agcgcgatcc gctcgacttc gtcctgtgga agcgcgcgaa ggcgggcgag ccgtcgtggc
     7561 ggtcgccctg gggcgagggc cggccgggct ggcatatcga gtgctcggcg atgagcatga
     7621 agctcctcgg cgagcacttc gacatccacg gcgggggaca ggacctgcag tttccccacc
     7681 acgagaacga gatcgcgcag tccgagggcg cgctcgcgga cggccggccg ttcgtgcgct
     7741 actggatgca caacgcgttc gtgcgcatgg acgaggagaa gatgtccaag tcgctcggca
     7801 acgtcgtcac cctcccgcag gcgctcgcgc gccatgacgg cgaggcgctg cgcttcttct
     7861 tcgcgcgggc gcattaccgc agcccgcttt cgtacaccga gcgcaacgtc gaggatgcgc
     7921 gccaggcgct cgcgacgctc tacaccgcgc tgaagaccgc gcggtcggag gctgccgccc
     7981 cggactgggc cgagccgcac ggtgcccgct tccgcgcggc gatggacgat gatttcaata
     8041 cgccggaggc ggtcgcggtc ctgttcgatc tcgcgacgga ggtgaaccgg agccgcgacg
     8101 cggtcctcgc acggcagctg cgcgcgctcg gcggcgtgct cggactgctg gggcgcgacg
     8161 cctccgcgtt cctgcaggga gcgccggcga gcgaagccgg tatctcgagc gcggagatcg
     8221 agggcctgat tgccgcccgc aacgccgcgc gggccgcgaa gaattacgcg gagtccgacc
     8281 ggatccgaaa gtcccttggc gaacggggaa tcgtgctcga agacggtccc ggcggcacta
     8341 cctggcgccg cggctgatca gtacgcgaac cgccccgact tgccgatcgc ggtcagctcg
     8401 acgaacttcg agccgttgtg ctccttgggg ttgaagtcga cgacgtagcc gccggcgtcg
     8461 aagttctgca tcgagtcgag cgccttgatg agcgtttcac gcgtcaggtt cgggcccgcg
     8521 cggcgcatcg cttcggccag gaccttggcg gagatgtacg actcgagcga cgtgaacgag
     8581 aaggccctct tgccgagaag cttctcgatc gccgcctggt attccttgac gaccgggacc
     8641 gacgccttgg aataaggcgg gacgacctgc gcgacgacca cgccgctgcc ctggtccttg
     8701 agctcgcccg cgagcgcgct cgcgcccacg aacgaggtgc tcgcgaactg cgccccggat
     8761 ccgccccgcc gcgcctgctt gatgaaatcg gcggtggtgc ggtagagcgt cgttgcaatg
     8821 accacgtcgg gctgcgccgc cagcacgccg ttcacggcgt cgcccagcgt agtctggttt
     8881 cgctgcagcg tgccgatcgc gaccggcttc aggctgcgcc gggccagcgc cgcctgcacc
     8941 gccgcgaggt tctcctttcc gaccgtgtcg tcgtagtgga tcaccgcgaa cttgctcatc
     9001 cccgtcgtcg tgtagaactc gacgatcttc tccagctcgt cggcgtagct cgcgcgcacg
     9061 ttgtacacga gcgggttgaa cttgcgcatg gggtctgcgc ccgtgaacgg cgcgacgaac
     9121 ggaacgcggt gcttctcgac gaaaggcagc gccggcatgc tcagcgtcgc gctcgcgtac
     9181 ccgaacaggg cgacgacgcc gcgctcctcg atcaggatgc gcccgttctc gcccgatttc
     9241 ttggcgtcgt tcgcatcgtc cagcgtgacc atctggatct tgcggccgtg gatgccgccg
     9301 gcgtcgttga tcctgcggaa gtaggcgttg gcgccgtcgc ggatgtcctc gccgaactcc
     9361 ttgttgctgc cgctcaaggg gccggactgc cccacgagga tggtggtctt ggtgacgcct
     9421 tgcgccggcg cgcccaccgg cagtgcggcg gcgacgatga acaggctgat caggctgatc
     9481 gtggatacac gcatggcgcc ctcctcttgg ctctgcgtgg ggcgcctgcc ggcgcccgca
     9541 ccgttcttat acctatttca ggaacttacc ctgccttccg atcatagtga ggtcgacgta
     9601 ctgcgacccg ctgtggttgc tcggcgagaa cgagacgttg aatccgccca ggtccacgct
     9661 gttcatgctc tcgagcgcgg cgacgagctt ctcgcgcgtc aggtcgcggc cggcgcgctt
     9721 caggccttcc accatgacct tggccacgat gaagccctcc aggctcgaga agttgtagtc
     9781 cgtgttgccg gcctgcttga gcacctgctg gtactccttg acgatcggca ccgccgggct
     9841 ccacgggaag ggcacgacct gcgagatcgc gaccccgtag ccttcgtcct tcagcgcgtc
     9901 cgagagcgcc ttgctgccga cgaaggagac gttgtggaac tgcgcgacgc tgcccgccgc
     9961 gcgcatctcg cgcacgaact cggcgatcga ggtgtacgcg gagatcatga tcaccgcgtc
    10021 gggcttggcc gcgtcgatgg tcttgaccgc gtcctgcacc ttggtggtgt tgcgctccac
    10081 cttgccgagc gccgagatct tgaggctgcg cttcgtcatc gcgatctcga cgcccttgag
    10141 gccggcctgg ccgtaggcgt cgtcctggta gaagaccgcg atgttcttgt tgccggtcga
    10201 tacgagctgc tcgacgatct tctccgtctc gtcgtagtag ctcgcgcgca cgttgaagat
    10261 gtaccggttc acgggcgtgc gcagcacctc ggcgccggtg aacggtccca cgagcggaac
    10321 cttggcctgc gtgaagatgg gcatcgccgc gcccgtcgtc ggcgtgccga cgtacgatat
    10381 cagcgcgaag accttgtctt cctcgatcag cttcttggtg ttctcgaccg cgaggttcgc
    10441 ctcgtacttg tcgtcgcgcg tcttgagctc gatcttgcgc ccgtggacgc cgcccttggc
    10501 attcacgtag tcgaggtacg ccttggtgcc gatgttcatc tggatgccca gctgggccgc
    10561 cggaccgctg aatgccgccg actggcccaa cacaatcgtc gtgggcgtga caccgggggt
    10621 ctgggcgaac gcggttccca ccgtcgcgca cacgcccgct agggtcgcta agtacctgat
    10681 attcattgtt cctccgacat tgcccgtctc ttatgcatct ggaagcagca actttcgtgc
    10741 cgcatgcacc cagcatattc ggccgccgcc gcgatgatgc gggtcggcgc ggttcctgaa
    10801 cggtcgcgga cgaggctact ccaaagcttt tctgttggga agcatcgcgc tcgcgttcga
    10861 gagcggtcgg gcgttggggg aaaccctatc gaggccctac tcggcgaaga attccttcac
    10921 tttcgccatc caagatttcg cgcgcggatt gtggcgctcg ctgtccttct ggctgagcgt
    10981 ctcgaactcg cgcagcagct ccttctgccg gtcggtgagg ttgaccggcg tctcgagcat
    11041 cacgtgacac agcaggtcgc cgtgctcgcg gctgcgcacg cctttgatgc ccttgccgcg
    11101 caggcggaag atttttcccg actgcgtctc ggccgggacg cggatcttcg cgctgccgtc
    11161 gagcgtcggg atctcgatgt cgcctccgag cgccgcggtc gtgaagctga tcggcatctc
    11221 gcagtggaga tcgtcgtggt cgcgctggaa cacggggtgg ggcttgacct ggatctgcac
    11281 gtacaggtcg ccggtcgggc cgccgttcac gccggcctcg ccttcgcccg cgaggcggat
    11341 gcgatcgccc tcgtcgacgc cctccgggat gcgcaccgac agcgtcttct gcttcttgac
    11401 gcggccagcg ccatggcacg tggggcaggg ctcggggatg accttgccgc tgccgtggca
    11461 gttggggcag gtctgctgca gcgagaagaa gccctgctgc atgcgcacct ggccgtggcc
    11521 gttgcaggtc gagcaggtgc gcggcttggt gccgggcttg gcgcccgagc cgccgcagac
    11581 gtcgcaggcc tcgtgcgtcg gaatgcggat gcgcgtctcg gtcccatgcg ccgcctgctc
    11641 gagcgtcacc tcgaggttgt agcgcaggtc cgcgccgcga aatacggagc tgcgtccgcc
    11701 acgcgcctgg ccgaagatgt cgccgaagat gtcgccgaag gcgtccgcga agccgccgaa
    11761 gccggcggcg ccggcgcccg ccatcccggc ctggggatcg acgccggcgt ggccgtagcg
    11821 gtcgtacgcg ccgcgcttgg cgccgtcgca gaggacctcg taggcttcct tcgcctcctt
    11881 gaacaattcc tcggctttgg gattgtcggg gttccggtcg gggtgatatt tcatcgcgag
    11941 gcgccggtac gccttcttga cggtctcctc gtcggcgtcg cggttgacgc ccagcacttc
    12001 gtagtaatcc cgtttcgcca tctcgtgctg ccccttacgc gcaaacgccg agttgagccg
    12061 cgcgctcgcg cgcgtcggtt cgaactcggc gagttaaggc ggctcgctgc gcgagccgct
    12121 cttaaccttt cttgtccttc acctcggtga actcggcgtc gaccacgttg ctgtcgtcgg
    12181 tcttcttcga gcccttggcc tcggcgccgg cctgctgttg ctgctgttgc gcatccgcgt
    12241 acatcttttc gccgagcttc tgcgaggcct gcgcgagcgc ctgcgccttg gcgtcgatcc
    12301 tgtccttgtc gtcggtcttg agcgcttcct cggcgtcctt gatcgcggcc tcgatcttcg
    12361 tcttttcgtc ggcgccgatc ttgtcgccat gctccttgag cgacttgcgc accgagtgcg
    12421 cgagcgcgtc gagctggttg cgcgcggtga cgacctcgag ctgcttcttg tcctcggccg
    12481 cgtgcgcctc ggcgtccttc accatgcgct ggatctcggc ctcgttcagg cccgagctcg
    12541 cctggatctt gatcttgttc tccttgccgg tcgccttgtc cttcgccgac acgtgcagga
    12601 tgccgttcgc gtcgatgtcg aaggtgacct cgatctgcgg catgcctcgc ggcgctggcg
    12661 ggatgtcggt caggttgaac tggccgagcg acttgttgcc cgaggacatg tcgcgctcgc
    12721 cctgcagcac gtggatggtc accgcggtct gcccgtcctc ggcggtcgag aacacctgct
    12781 gcgccttggt cgggatggtc gtgttcttct ggatgagctt ggtcatcacg ccgcccagcg
    12841 tctcgatgcc gagcgacagc ggcgtcacgt cgagcagcag cacgtccttg acgtcgccct
    12901 tgagcacgcc gccctggacc gccgcgccga ccgcgaccgc ctcgtccggg ttcacgtcct
    12961 tacgcggctc cttgccgaag aggtcgcgca ccgtctcctg gaccttcggc atgcgcgtca
    13021 tgccgccgac gaggatgacg tcctcgatct ccgagagctt caggcccgcg tccttgagcg
    13081 cggtcttgca cggctcgacc gtcttcgaga tgaggtcctc gaccaggctc tcgaatttgg
    13141 cgcgcgtgac gcggatcgcg aggtgcttcg ggccgttctt gtcggcggtg atgtagggca
    13201 ggttgacctc ggtctgctgc gaggaagaga gctcgatctt cgccttctcg gcggcttcct
    13261 tgagccgctg cagggcgagc acgtccgcgc gcaggtcgat gccctgctcc ttcttgaact
    13321 cgtcgcagag atagtccatg agccgctggt cgaagtcctc gccgccgagg aacgtgtcgc
    13381 cgttcgtgga cagcacctcg aactggtgct cgccttcgac ctcggcgatc tcgatgatgg
    13441 agatgtcgaa cgtgccgccc ccgaggtcgt agaccgcgat cttgcggtcg ccttccttct
    13501 tgtccagccc gaaggcgaga gccgcggcgg tgggctcgtt gatgatgcgc ttcacttcca
    13561 ggcccgcgat gcggccggcg tccttggtcg cctggcgctg cgagtcgttg aagtaggcgg
    13621 ggaccgtgat cacggcctcg ctgacttcct cgccgaggta gtcctcggcg gtcttcttca
    13681 tcttgcgcag cacctgcgcg gacacctcgg gcggcgcgat cttcttgccg cggacctcga
    13741 cccaggcgtc ggcgttgtcg gccttgacga tcttgtaggg catgaggtcg atgtccttct
    13801 ggacctcctt ttcctcgaag cgccggccga tgagacgctt caccgcgaac agcgtgttct
    13861 ttgggttggt gaccgcctgg cgcttcgccg gcgcgcccac caggatctcg ccgtcctcga
    13921 ggtaggcgac gatcgacggg gtcgtgcgcg agccttccga gttctcgatc accttgggct
    13981 tgccgccctc catgatcgcg acgcaggagt tcgtcgtccc gaggtcgatg ccgatgatct
    14041 ttgccatgtt cgttccttta ataaccttgt gcgggatatg gggatgaatg ctccgacttc
    14101 aagcagccgg gggcatggga ttcaggggtt tatcgctcat ttggcctggg acacggtgac
    14161 cagcgccggc cggatgacgc ggtcgttcag gaggtagcct ttctgcagca cgttgatgac
    14221 cgtgttgggc tcgcggtccg agggcagcat gctgatcgcc tggtgccggt gtggatcgaa
    14281 tttctggccg accgggttga tctccttcag gttcgccttc tcgaataccg cggcgagctg
    14341 cttgagcgtc agctcgaccc cgctgcggat cgcgtcggcg gacgcggtgt cggtggcgag
    14401 ggcggcctcg aggctgtcct tcacgggcag cagctcctgc gcgaaattct cgatcgcgta
    14461 cttgtgcgca ttcgcgacct cgacctgcgc gcgcttgcgc acgttctcgg tctcggcctt
    14521 ggcgcgcagc cacgcgtcgt ggtgctcctg cgccttgagc tcggcctggc gcagcagctc
    14581 ttcggccgcg gggatggcct cgggctcgcg ttccgcgcgc ttctgctcgg tttcgcgcgc
    14641 ctccatttcg gcgtcggcgt tgccgatcgg cgcctggggt tcgttcgttg ccattcagaa
    14701 ctccggatat cgtcagcggg cgcggggctg ctcggccccg ccggattcga caatgctagc
    14761 ttgggcccga cccggggatt tcaagttccg cgcctcggcc tccgggccga tccatcccgt
    14821 gaggttttcg agcacgatcg atgccagggc gccgatccag gcctcgtgct cgttcagcgc
    14881 cgggaccagc cggaactcgc ccccgcccgc ggcgaggaag gtctgcttgc cctcgatgcc
    14941 gatctcctcg agggtctcga ggcagtccgc gacgaagccc gggcagacga cgtcgacccg
    15001 acgcatcccg gcgcggccga gatcggcgag cgtcgacgcg gtgtagggtt tcagccactc
    15061 gccggcgccg aatcgcgact ggaacgtcat ctgccatcga tcctcgccga tgccgagcgc
    15121 gtcggcgagc agccgggccg tctgccggca ctcggactgg tacgggtcgc cacgctcgac
    15181 gctgcgctgc gggagcccgt ggaagctcat gaccagcctg tccggccgcc cggcggcctg
    15241 ccagtgctcg cgcacccgcg cggcgagcgc gccgatgtag gcgggatggt cgtgaaagcc
    15301 gcgcacgatg cgcagcgccg ggacgttccg ggtgcgccgg atgaaagcgg cgacctcgtc
    15361 cagcacgctc gccgtcgagc tcgccgcgta ctgcggataa agcggcacga cgagcacgcg
    15421 gtcgcagccg tcgcgcttca gccgccgcag cacctcggcg atcgcgggcg cgccgtaccg
    15481 catcgccgcg tcgaccgcga gcggcgcgcg gatacgcgcc gccagggtgc cgcgcagcag
    15541 cccggcctgc ttctcggcat ggaccttgag cggcgaccct tcgggcgtcc agatgctcgc
    15601 gtagcgccgc gccgaggccc tcgggcgcag ccgcagcacg acgccgtgca ggatcggcca
    15661 ccacagcgcg cgcgggatct cgaccacgcg cgggtcccac aggaactcgg cgagatagcg
    15721 ccgcaccgac gcggcggtcg gcgcgtccgg cgtgccgagg ttgaccagca gcacgccggt
    15781 cttcgcgggg ctgccgtgct ggcgctcggg ctccgggtcg tagcgcacgg cggaggggcg
    15841 ccgcctcagc cttcgagacg gtgctgcgac agggcgctcg acaggagctt cgcggtgatg
    15901 tcgacgatcg ggatgacgcg ctcgtaggcc atgcgcgtcg gcccgatcac gccgaccgtg
    15961 ccgacgacct gcccctcgac ctcgtagggg gcggtgacga cgctgcattc gtcgagcggc
    16021 gcgatgcccg actcgccgcc gatgaagatc tgcacgccct gcgagcggct cgagagatcg
    16081 aggatctgca tgagcaacgc cttcttctcg aacagctcga acaactcgcg cagccgcgtc
    16141 atgttcgccg cgagatcctg cgagtcgagc aggttgcgct cgcccgacac cgcgtacgcc
    16201 tcggtcgact ccgataccgc ctggctcccg gcctcgaggg ctgcggtcat gagcgcagtc
    16261 atgtcctcgc gcagccgctg cagctcgtcg cgcacgcgcc ggtggatctc gtcgaaggtg
    16321 agaccggcgt agttctgatt caggaagttg gcggcgtacg tcagctccga cggcgtgtag
    16381 gtgcggtccg tgaacaggat ccggttctgc acgtcgccct cggcggtgac gatgatgagc
    16441 aggacgcgct tgtcggacag agcgaggaac tcgatgtgcc ggaacgcagg cgacttgcgc
    16501 ttgctcgcga ccacgacgcc ggcgaagtgc gtgagctccg acaggagctg cgaggcgctc
    16561 gcgatcaggt tctgcggctg ccccgggttc aggctcacct cgacctggct gatctcgatc
    16621 tgcccgagcg gcttgatggt aagcagcgag tccacgaaca gccggtagcc ccggggcgtc
    16681 ggcacgcggc ctgcggaggt gtgcgggctc accacgaagc ccaggtcctc caggtccgcc
    16741 atcacgttcc ggatcgtcgc cgggctgagg tcgaggcccg agaagcgcga cagcgcccgc
    16801 gagccgaccg gctcgccttc ggcgatgtag cgctcgacga gcgtcttcag caggacctgg
    16861 gcgcggcggt cgagttgcat ggttgcgaat tctaccgaat cccggagccg gagccggtcg
    16921 tccttttgtc cgggctgtac gttcatgatc taatgccgga catgcccgct ttcaagacca
    16981 ttgcgctgat cggacgccac aatgcgccgg acatcggcgg ggcggtgctg gcgatggcgc
    17041 atttcctgga gcgcgagggg tgcacggtgc tgatcgagcg ccgcaccgcc gagagctgcg
    17101 gcctgcaggc gccgcccgcg catgcggact acaccgagat cggcgcgcgc gccgatctcg
    17161 ccgtcgtgct gggcggcgac ggcagcatgc tgaccgcggc gcgcagcctg gccgcgaagc
    17221 gcgtcccgct ggtgggggtg aaccagggcc ggctcggctt cctcaccgac atcgcggcgc
    17281 gggagatgac cgagaccatg gagcagatcc tcgccgggcg ttattcgctc gaggaacgca
    17341 ccatgctcgc cgccgaggtc cgccgcggcg acacggtggc gttctccacg ctcgcgctca
    17401 acgacgcggt cgtgaacaag ggcgccgtcg ggcgcctgat cgagttccac gtccacatcg
    17461 acggcgagtt cgtctacgac ctgcgctccg acggcctgat cgtgaccacg ccgacgggct
    17521 cgaccgcgta ttcgctctcg tcgaacggcc ccatcctcga gccgcgcgtg cccgcgttcg
    17581 cgctggtccc gatctcgccc cacacgctgt cgaaccggcc ggtcgccgtc agcgaccgct
    17641 cggtgatcga gatcaccctg cggcactcga tcgacgcacg cgtgcacttc gacggacagc
    17701 cgcagttcga cctgcacgac ggcgaccggg tcacgatccg gcgcgccgcg gagaccacga
    17761 ccctggtgca cccgcccggc tacagctact acggcatgct gcgggagaag ctgcactggg
    17821 cgggcggcgc gctgtagcgg catgctgacg cggctctcca tccgcgactt cgcgatcgtc
    17881 gaccacctcg agctcgactt cagccgcggc ttcaccgcgc tcaccggtga gaccggggcg
    17941 ggaaaatcga tcctgatcga cgcgctgtcg ctcgcgctcg gcgagcgcgc cggcgccgag
    18001 ctggtgcgcg cgggctgcga gcgcgccgag gtgacggcgg agttcgcgat cgatcgcatc
    18061 gcgccgctgc agtcctggct gcgcgaccag gcgctcgagg acgacggcgg gagcgtgctg
    18121 ctgcgccgcg tgatcgacgc gggcggacgc tcgcgcgcct tcatcaacgg ccgcgccgcg
    18181 acgctcaacc agctgcgcga ggtgggcgaa tggctggtcg acatccacgg acagcacgcg
    18241 caccagtccc tgctcaaagg cgagatgcag cgcgcggttc tcgacgacca cgccggcctg
    18301 tcgcagctgg cggacgagac ggccggcgcc ttccgcgaat ggcagaagct gtgccgcgcg
    18361 cgcgccgagc accagacgca cgcggccgcg cgcaacgccg agcgcgagca gctgtcctgg
    18421 cagatcgagg agctcaggcg cctcgcgccc gcggccggcg aatgggacgc gatccagtcc
    18481 gagcacgggc gcctggcgca cgcggcgagc ctcatcgaag gcgcgcaggg cgcgatcgac
    18541 gcgctgtcgg agtccgagac cgcggcgctg gcggtgctct cgggcgtgct gtcgcgcatg
    18601 cggccgctgg tcgactacga cgccgcactc aaggacacgg tggggctgct cgagtcgggc
    18661 gaggccaatc tgcgcgaggc cgcctattcg ctgcgccact acgtcgatcg catcgagctc
    18721 gaccccgcgc gcctgcgcga ggtcgaggcg cgcctcgagg cgctgcactc ggccgcgcgc
    18781 aagttccacg cgcgtccgga ggcgctgccc gagcggctcg ccgagctcga agcccgcctc
    18841 gccgagctcg acaccgcgac ggatctggag gcgctcgcga agcaggagca ggccgcgcgc
    18901 tcgcgctacg acgcgctcgc cgcgcggctc tcggcggagc gcaggaaggc ggcggcgaag
    18961 ctctcccggg agatcaccgc ggcgatgaag gagctggcga tgggcggggg acgtttcgag
    19021 gtcgagctgc gctcgctgct gcccgacggc agcaccgggg gcaacgagca ggtcgagttc
    19081 ctggtcaccg ccaatcccgg cgtcgagccg aggccgctcg cgaaggtggc ctcgggcggc
    19141 gagttgtcgc gcgtgagcct cgcgatccag gtcatcacca gcaaggcggc gcgcgtgccg
    19201 acgctcatct tcgacgaggt cgacgccggc atcggcggcg cggtcgccga ggtcgtcggg
    19261 cgcaagctgc gctcgctcgg cgaagaccgg caggtgctcg tcgtgacgca cctgccgcag
    19321 gtcgccgcgc aggccaccga gcagtggtcg gtcgcgaagg gcgccggcgg cgccggcgtg
    19381 cgcagccggg tgaccgtcct cgaccgcaac tcgcggatcg aggagatcgc ccgcatgctc
    19441 ggcggcgtgg ccatcacggc gaccacgcga aaacatgccg ccgaaatgct cgggttgcgg
    19501 tagtattttg ccgtctgagt ggaggagacg gaatgaagac aagaaaacat tcgcgcggcg
    19561 tgggacagat cgtctttctc gtggtgctcg cgctgctgct cgccgggggg gtcctgttcc
    19621 tcgatcgata caacgcgggg acccgcgaca tgctgaccgt ggagacgcgc ggcctgcaga
    19681 tgatttccgc gctgacccgg tacaagctcg agaacggcgg ctatcccgac agcctcgaca
    19741 agctcgttcc caagttcacg cctgccgtca gcgcctgccc gagcgggact ccgatcgagt
    19801 accggctgag tggcggcaac tacgtgctga cctgcgagaa cgtcgtgctc aagatgcagc
    19861 cgtatcacta cgactcgcgc acccgcgcat ggggcagctg acgcgccgtt ggtttgtggc
    19921 ccccggggtt ttcggctaac attccacgct cccaggcgcg tagctcagtt ggttagagcg
    19981 catccttgac atggatgagg tcgttggttc gactccaatc gcgcctacca tcatttctcg
    20041 cttcaccgca ctcgcatccc tgatcgtgct ctcggcttgc agctcgacgc cggtgtggca
    20101 gccctcgggg tacaagccca gctcggtgca gctgctggtg acgcattacc tgcctcccgg
    20161 ctgcgacaac ggcgcatgcg ccatccgcca cggcacgagc ggggattgca tcatctacat
    20221 gctgccgaag ctcgagggtc gcaaggacgt gctcgaccac gagctgtgcc actgcctcgg
    20281 ctacgaccac ggcaaggaag taccgggcct gctggtggtg ccgacctgcc cggtggacac
    20341 cgggcgggtg gagtacaagg tcagcagctg aggcgccgcg cggctagccg ccgctcggac
    20401 tcctgcgctg ggcttcggcg tgcgcttccg gagcgggcgc gtcggcgacg gcatcgtcgt
    20461 ccttgatcgg cggatcgtat tcgatcttct cgcgcgtgcg cgatgcgagg tggagcagcg
    20521 tcatttcggt ccgcccgatc ttctcgatct tgtactggcc gtcgagcgtg tcgcccttct
    20581 tcacgggcac cagctcctcg cccttcatca ggtaaacctc gacggagttg ccgacatgca
    20641 gccgccccgc gaagcggtaa ggcatcggcg gtgcgacggg cttggccggt gccggcggtg
    20701 gcgccgagac cacgggcgct gggagcgcgg gcggctcggt gttgaacgga tcggcaccca
    20761 tctggccgag ggtcgcgcgc tcgggtatct cggacgccgc ggcgggctgc ggcgcggccg
    20821 gggacggctt cgccgtggac gcgacgctcg ccggcgcatg ctgcgcatcc ttgccgccga
    20881 atgccgccca ggcggccact ccggcgagcg ctgtaaacaa tagggcgagt cgggttctgc
    20941 gttgcatggc gttgctccct ggtgcttttg tcggttttgc tatgtcggtc gtgtcgttcg
    21001 atacctagcc cggcggccgc cccggtgacg cctgcggcac cggcagcgcc gcgcccgggg
    21061 cgccgggctg gtagacgaat ttccagtcgg agtacttcgc ggcgccttcg aacgccgcgt
    21121 ccgcgaggcg aaagcccgcc gccttcaccg gctgatcctc ggagcggctg tagacgccca
    21181 tgatgccgcc gtcgggcgcc ttgacgaggc cccattcgtc ggatcccatg atcgggtccg
    21241 gatagcgctt gcgcaggtag cgccggacgg tgacgtcgcg cgggtccctc acgaggtcgt
    21301 cgagcgagcg cgggtacagc ccgccgccgc gcttggtgta cgcctcgatc gcgaggcggt
    21361 actggttgcc tctccacagc agctcggact cgcgctcgcg cagcacggcc gtgcggtaga
    21421 cctggccgac cttcgccatg ccgaggctca tgaaggcgac gacgaacagc accgtcaggt
    21481 acgtgaaacc gcgctgcgtg ccgaacgggc gcttcatcga tcgctcacag gtccgcgtac
    21541 gacgtaccgt cggcggtctt gccgtcggcg ccgctcttca cgtcgctgac gcccgtcgcg
    21601 tccccgtcgg ccggctggac cgcgacccag ctgtccttct tctcggtgat cggatcgacc
    21661 ggcacgctgc gcaggtactt ctccgagacc agcgcgtcca gcgtatcggg atacttgccc
    21721 ttgtcgccgt agtacttgcc gatggcgtcg cgcatctgga acaggtcctc cttcagcacg
    21781 gcctccttcg acttgtcgag gctgccgaag tagcgcggca cggcgatggt gaggagcagc
    21841 gccacgatcg ccagaacgac cagcagctcg atcagggtga atcctgtcct gagcagggtc
    21901 gaagggccga ggtgtttgat cttcatggcg tcaccactcc ttgtacgggc gtccgtcgct
    21961 gccttttccg ggtgcgcgcg agaacacgtc gaagacgtcg gcgccgtcgc gcggctcgtc
    22021 cgggtcgctc gcatagctgc gcttgcccca ggtcgccgcg gccgagagcg acgcgtccgc
    22081 gttgaagggg tcgcgcggaa cgcggcgcag gaaaaagatc ttctcgcgct tcgggctttt
    22141 ctcgttctcc acgccctcgg cgagctgctc cagcgacttc gggtagcccg tctcgtcgat
    22201 cgacttcttc acgcggcctt cgtcccacgc gagcttgtag gcgtcgatgg cgtcgcggat
    22261 ctggcgcagg tcgcggcgca gctcgctttc cttctcgcgc cgcacgacca gctcgctcac
    22321 cgggaacgcg gccgacgcga ggagggccac gatcgcgacc gtgatcatga gctcgatgag
    22381 ggtaaagcct ttagggcgct tgctcacgct cgctccttca ctgtttgggc gccgcgtacg
    22441 gctgctgctg cacgccgccg aacggcacca tggtgtttcc gggcggcgcg ggggcgggcg
    22501 ccggcgtcgg agccggcgcc gggcgctgcg gcggctggac cggcggctgc tcgacaatca
    22561 tgggcgacgg tccggtgccc acgaccgccg gtccctcgac cgacgtctcc gtgcccgccg
    22621 agaattccgt ggtgcgcggt cccgggcgct cgatcgtgcg cacgatgtgc ggcgtcagca
    22681 gcagcacgat ctcggtcttg ttgcgcgtat cgctcgtctg cgagaacagc ttgcccagca
    22741 cgggcatgtc gcccaggccc gggacgcggc tcgcggtgcg gcggtcctcg tcgttgatca
    22801 ggccggcgag cacctgcgtc tcgccgtcgt gcaggcgcag gacggtcgtc gcattgcgcg
    22861 ttccgatctg gtaggcgagc gtgttgcccg tggagcccct caccgtgttc acgatgttgc
    22921 tgacctcgag gccgatgttg atgcccacgt cgtcctcgag atacacgatc ggctcgacgt
    22981 cgagcttcag gcccacgtcg aggtagctca ccgactcgga cacgaagccg cccgcgacgg
    23041 ccgccgtggt cgtgatcacc ggaacacggt cgccgatgtg gatgcgggcc ttctccttgt
    23101 tcttcacgcg gatgcgcggg ttcgcgagca ggttggtccg gccgtcctgc tggcgcaggt
    23161 tcagcaggaa caggggatcg gagaagttga gccgcaccag ccccgaattg cggttcaggt
    23221 actcgggcag cgagatctgt ccgggcacgc cggccgcgcc ctgcacgccg gcggagaccg
    23281 agtccggaaa cttcagcccg agatcgagca ggcggttcgc gccgacctcg agcacctcga
    23341 cctcgagcat gacctcgggc tcggcgatgt cctgggccgc gatgaggcgc tcggcaaggc
    23401 gcacggcctt cggcgtgtcg cgcatgacga gcatgttcag cttctcgtcg acgaacacgt
    23461 cccgggtctt caggatcgtg cggatcatgg tcgcggtctg cttcacgtcc gcgttcgcga
    23521 gatagaagct cttcaccacc agctcttggt agtcgcgctg cttctgcggc gtgttgggga
    23581 agaccagcgc ggtcgtctcg ttcagcacct tgtagtcgag ctggttcgac gtgagcacca
    23641 ggcggattgc ttcctcgatc ctggcgtcct tcacgacgat cgtcgtcttc tggtcggcct
    23701 tcacgtcctt gtcgaacatg aagttgatgt tcgcggcgcg gccgatcacc tcgaacgccg
    23761 agcgcagcgg cacctcgcgc agctcgagcg agatcggctt cgtcgcctgc accttgagct
    23821 gcgggacggc gggcttcgcc gtcctttcct cgatctgccg ctgcaggcgc tgcgcctcgc
    23881 ggttgttgcg gttcgcggag agcagcggcg cgagcttctc ctcggcctcg cggtacttct
    23941 cggccttgac caggtcctgc acctcgttga ggaccgcgcg gttgcgctcc tcgacgtcga
    24001 gctgcgacag gccgttcttg gcgcgcacgt tcccggggtc gtacttgagg accttgcggt
    24061 agagctcgcc cgccgcggtg tactgcgacg cggcgctcag ggcctgggcc tgtccgagcc
    24121 actgcccgat cagcaggtcg cgctcgtgga agtactcggt ccggaatgtc cggtcctcgg
    24181 gatgctcgcg ctgcgcctgg tcgagccgcg cgagcgcctc ctcgccgtgt cccgagccga
    24241 gcagcgtgcg cgactcttcg acgacgggat ccgtcttgat cgacgcgcac gcggacagca
    24301 gcgcgaggca ggcgatcagg agcagggcgc agcgtcgtga ggctttcgtc atcatcggat
    24361 ctcgttgcga ttgctgggac ggaagaacag cgtgaggcgg acctgcgcgt cgagctgcgt
    24421 gtcgcccact ttcttgcgct cgaagcgcac ggcgtcgacc gccgcgtacg gcatctgctg
    24481 cagcgtgtct gacaggaagc cgcggatgtc gggatagctg ccgcgcagcg gaagcgcgat
    24541 gcggtacgac gcgaggccgg gaccgcgctg ctcgaggcgg tactcgccct gcgacaggtc
    24601 gagccgggag gcgcgcgcga ttccatagag gcgctcgagc tcgtcgggca ggcggtccat
    24661 ggcggggaac gcctggtaga aacgtccgag ctccgggccg cgcggcgccg agacggtctg
    24721 gatgccggca cgggcgcgcg tctgctcggc cgcgaggcgg cgcgcctgca gctcgcgctc
    24781 ggcgggtcgc acggacgaga agtagaacgg caggcacgcg accagcacgc cggcgccgag
    24841 cacgccggga acgccgagcc ggcgcagcga gttgcggagc gacttcacgc cgatcttcat
    24901 tttggcgctc ccagagaggc ctgcaccgag aactggatcg gccgcgacgg gttgtccgtc
    24961 tggacctggt gactcacgat gtagacgttg gaaaggccct gcgtctgggc gatgcgtcgc
    25021 acgtactcga gcatcgcctc gcgcgtcttc gcctcggccg tgagcttgag caggcgctgc
    25081 tgcgcgtcgg gctgcatgtt cagcacgccg acgtcgcgca tcgaagcccc ttcgatggcg
    25141 cgaacgatgt cgctccaggg cagggtcagc tgccgcacca cggtctcggc ctgcttcagc
    25201 tcctcgtcgt tcgttttggc cggctcgcgc gtctgcgtca ggtcgagcgc gccctggacc
    25261 gcggccagcg ccgcgatatc ccg
//
LOCUS       DATDMW010000088         2916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1020844, whole genome shotgun sequence.
ACCESSION   DATDMW010000088 DATDMW010000000
VERSION     DATDMW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020844"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VI319_03760"
     CDS             <1..387
                     /locus_tag="VI319_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY6862991.1"
                     /translation="VGIDLADLRGYHYHTGVVFAAYAPGVSNAVALGGRYDEVGKAFG
                     RARPATGFSMDLRELARIAPKGRERGGICAPYSRDRKLADAVAKLRRAGESVVVDLPG
                     HGGSRAELGCDRALVKRGGRWPVKKL"
     gene            415..1707
                     /locus_tag="VI319_03765"
     CDS             415..1707
                     /locus_tag="VI319_03765"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY6862992.1"
                     /translation="MAKNVVVIGTQWGDEGKGKVVDWLTDHAQGVVRFQGGHNAGHTL
                     VIGGEKTVLHLVPSGILRSGVTCYIGNGVVLSPQALVDEMAALERAGVDLAGRLRVSE
                     ACPLILPYHAAIDVARESRQGDAKIGTTGRGIGPAYEDKVARRAIRLQDLFHPKRFGA
                     KLEPLLEYHNFVLTKYLGAKAVEYGRVRDEALALAPRLAPLVADVARALYDANHRGEN
                     LLFEGAQGTLLDVDHGTYPYVTSSNCVAGAAAAGAGVGPQMLHHVLGITKAYATRVGA
                     GPFPTEQDNAVGELLRRRGQEFGATTGRPRRCGWFDAPALRRAIQINGVSGLCITKLD
                     VLDGMETVRLCTGYRIEGVMHDLLPLGAEDAARCEPVYEDMPGWRESTVGISEYGRLP
                     ARARAYLERLQALCGVPVDMISTGAERKETIVLRHPFH"
     gene            1859..2158
                     /locus_tag="VI319_03770"
     CDS             1859..2158
                     /locus_tag="VI319_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY6862993.1"
                     /translation="MNNLATEAMPRDGAMTAEERKLADLIVSALNLDVQVADIDPDAP
                     LYGDGLGLDSIDVLEIALAISKAYGIKLRSDDDNNRKIFASLRSLSQHIQRHRTK"
     gene            2206..>2916
                     /locus_tag="VI319_03775"
     CDS             2206..>2916
                     /locus_tag="VI319_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6862994.1"
                     /translation="MADLPLIRAGRGSIFAYRRGRALGVEQYLRDVRRLAAQLPPRRY
                     VLNACADRYAFAVAFGAALVRGQSNLLPPNHTPDLLAKLKASYPDQYCLCDTDVRHPG
                     LETLVVPLETPADGASERVPAIPESHVAAIVFTSGSTGAPSEHVKLWGSLVRSALGEA
                     ERLGLGADSGWTLLGTVQPQHMYGFESTVLIAMQGGLAMHAGRPFFPADVCAELEALP
                     RPRALVTTPVHLAALLGEP"
BASE COUNT          423 a         1039 c         1046 g          408 t
ORIGIN      
        1 gtcggaatcg atctcgccga tttgcgcgga taccactacc acacgggcgt agtgttcgcg
       61 gcgtacgccc ctggcgtctc gaacgccgtg gctctcggcg ggcgctacga cgaggtcggc
      121 aaggcgttcg gccgggcgcg ccccgcgacc ggcttcagca tggatctgcg cgagctggcc
      181 cggatcgcgc cgaaaggccg agagcgcggc ggtatctgcg cgccgtattc gcgcgatcgc
      241 aagctcgccg acgcggtggc gaagctgcgc cgcgccggcg aatcggtcgt ggtcgatctg
      301 ccgggccacg gcggctcgcg cgccgagctc ggctgcgacc gcgcgctcgt caagcgcggc
      361 ggacgctggc cggtgaagaa gctctagccg cgccccgcgg tgtagactcg gctcatggca
      421 aagaacgtcg tcgtgatcgg cacccagtgg ggtgacgagg gcaagggaaa ggtcgttgac
      481 tggctcaccg atcacgcgca gggcgtggtg cgcttccagg gcggccacaa cgccgggcac
      541 acgctggtga tcggcggcga gaagacggtg ctgcacctcg tgccctccgg catcctgcgc
      601 tcgggcgtca cctgctacat cggcaacggc gtcgtgcttt cgccgcaggc gctcgtcgac
      661 gagatggccg cgctcgagcg cgccggcgtc gatctcgcgg gccgcctgcg cgtttccgag
      721 gcgtgcccgc tgatcctgcc gtaccacgcg gcgatcgacg tcgcgcgcga gtcgcgccag
      781 ggcgacgcca agatcgggac gacggggcgc ggcatcggcc cggcgtacga ggacaaggtc
      841 gcgcgccgcg cgatccggct gcaggacctg ttccacccga agcgcttcgg cgccaagctc
      901 gagccgctgc tcgagtacca caacttcgtc ctgacgaagt acctcggggc gaaggcggtc
      961 gagtacggcc gcgtccgcga cgaggcgctc gcgctcgcgc cgcgcctcgc accgctcgtc
     1021 gccgacgtcg cccgcgcgct gtacgacgcg aaccaccgcg gcgagaatct cttgttcgag
     1081 ggggcgcagg gcacgctgct cgacgtcgac cacgggacgt acccgtacgt cacctcgagc
     1141 aactgcgtcg caggcgctgc cgccgcgggc gccggcgtcg ggccgcagat gctgcaccac
     1201 gtgctcggca tcaccaaggc gtacgccacg cgcgtcgggg cgggcccgtt cccgaccgag
     1261 caggacaacg cggtgggcga gctgctgcgc cggcgcgggc aggaattcgg cgcgaccacc
     1321 ggacgtccgc gccgctgcgg ctggttcgac gccccggcgc tgcgccgtgc gatccagatc
     1381 aacggcgtgt ccgggctgtg catcacgaag ctcgacgtgc tcgacggcat ggagacggtg
     1441 cgcctttgca ccggataccg gatcgagggg gtgatgcacg atctgctgcc cctcggcgcc
     1501 gaggacgccg cgcgctgcga gcccgtctac gaggacatgc cgggctggcg cgagagcacg
     1561 gtcggcatca gcgagtatgg tcggctgccg gcccgtgcgc gggcctacct cgaacggctc
     1621 caagccctgt gcggggtgcc ggtcgacatg atctccaccg gcgccgagcg caaggagacc
     1681 atcgtcctgc gccacccgtt ccactgaacc ccgggggcgg ccgagtcctc cttttggagc
     1741 acttttgcgc accgaaattg ggccttggcg ggggtccacg gcgatataat cgtcgctggc
     1801 cgccgggccc ggcgctgggg ccgcacccgg gatagggccg atttcaggga caggggacat
     1861 gaacaacctc gcaacagaag cgatgccgcg cgacggcgcg atgacggcgg aggagcgcaa
     1921 gctcgccgat ctcatcgtga gcgcgctgaa tctcgacgtc caggtcgcgg acatcgaccc
     1981 cgacgcgccg ctgtacggcg acggcctcgg gctcgactcg atcgatgtcc tcgagatcgc
     2041 gctcgcgatc tcgaaggcgt acgggatcaa gctgcgctcg gacgacgaca acaaccgcaa
     2101 gatcttcgct tcgctgcgca gcctgagcca gcacattcag cggcaccgca ccaagtagat
     2161 ggagcgcccg ggctcgcgcg ggcgaaaccc gggcccggtc cagcgatggc agaccttcca
     2221 ctgatccgcg ccgggcgcgg gtcgatcttc gcgtatcgcc gcggcagggc gctcggcgtc
     2281 gagcagtatc tgcgcgacgt gcgccggctg gcggcgcagc tgccgccgcg ccggtacgtg
     2341 ctcaacgcct gcgccgaccg ctacgccttc gcggtggcct tcggcgcggc cctcgttcgc
     2401 gggcagtcca acctcctgcc gccgaaccat acccccgacc tgctggcgaa gctgaaggcc
     2461 tcgtaccccg accagtactg cctgtgcgac accgacgtgc gccacccggg gctcgagacc
     2521 ctggtcgtgc cgctcgagac gccggccgac ggcgcgagcg agcgcgtgcc ggcgatcccg
     2581 gagtcgcacg tggccgcgat cgtcttcacc tcgggatcga ccggggcgcc gagcgagcac
     2641 gtcaagctct ggggcagtct cgtgcgcagc gcgctcggcg aggccgagcg gctgggtctc
     2701 ggcgcggact cgggatggac gctcctcggc acggtgcaac cgcagcacat gtacgggttc
     2761 gagtcgaccg tgctgatcgc gatgcagggc gggctcgcga tgcacgccgg acgcccgttc
     2821 ttccccgcgg acgtgtgcgc cgagctcgag gcgctgccgc ggccgcgcgc gctcgtgacc
     2881 acgcccgtcc acctcgcggc gctgctggga gagccc
//
LOCUS       DATDMW010000089         3434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_24707, whole genome shotgun sequence.
ACCESSION   DATDMW010000089 DATDMW010000000
VERSION     DATDMW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24707"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="VI319_03780"
     CDS             <1..1758
                     /locus_tag="VI319_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6862995.1"
                     /translation="SRGRIRFPERALRFESPIGAVVIGTAGDDVHGADAALIVDPGGN
                     DVYERAPVTGGGVSVIVDLGGDDRYRGSDVAIHGLSAIVDLAGDDRYAMGGAGIAAAV
                     AGVSLVLDFAGDDVYEAGIFAQGAAVFGLGALVDLGGNDRYRVQAAGQGFAMTGGVGL
                     LWDRGGDDSYSAAGMPDAFDRGGGTSWAQGVGFGFRTALGGGIGILRDEAGDDRYEAE
                     MFAQGTSYWYGLGLLWDGGGRDVYRAVRYAQGNGVHQAVGVLRDEGGDDRYEMKIGVG
                     QGMGLDLAVGVLFDAAGDDFYGAPAYAQGNATANGIGIAFDAGGADRWHASADRRVWG
                     VAEPLRGLPSVGVIVYDAAGSIFDRDGKVVPAPAGATLEDPVEAAPAPPTASCPLTVA
                     APPEDDRPLAEMLRRLTPGVAANAIDPMQFARIRARLSRGIQAAMGQLLPAADDFDIA
                     YSFAAALRCTLAMAPAADAPAMWDELEEALLESPRGPFTGAVIGALRERPPPAAQMRR
                     ILATLERDPACGVRVVTLQAREAVADEASRAALVSAAQDALRSPCWQLQSAALGVLRR
                     SGAAPAPDARLPGFLRRAP"
     gene            complement(1767..2798)
                     /locus_tag="VI319_03785"
     CDS             complement(1767..2798)
                     /locus_tag="VI319_03785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY6862996.1"
                     /translation="MANASPVELGRDPLGAYRAHIMYSLATAAVLLLVPFAVNAFIQG
                     NAALAIGILGGVAILGINAIAVYLKRPPPIPLIFLLVPLVPAIGLSLKIQGFYGALWC
                     YPSVLLFTFSLSRRMANVCNLLMLVLISALVYHFIGPDYTIRFSATLALTVVLANLIL
                     GIVFDLHRRLLDQANVDPLTGAFNRRHMERCLSDAIERQRRTSAPASLLLVDIDHFKH
                     INDDFGHAAGDDVLRAVVELIGKRSRKLDLLFRIGGEEFMLLLPDTRESAAAVVAEQL
                     RASIEASRPGQAARVTVSIGVSELRPGETMDSWMKHADEALYAAKNAGRNRVAPTGTL
                     PLRSAESPA"
     gene            complement(2803..>3434)
                     /locus_tag="VI319_03790"
     CDS             complement(2803..>3434)
                     /locus_tag="VI319_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY6862997.1"
                     /translation="YDSNRYTIWGMLTRLGCEVIDLGVVRDDPAKLEAAFRDAADRAD
                     AVVTSGGVSVGEADFTKQMMAALGEVVFWKIAMRPGRPMAFGRIAARGRSAYLFGLPG
                     NPVAVMVTFYHFVRGALLHLAGCADCELPLLRAKSQVAMRKKPGRTEFQRGVLERRDG
                     EWTVRLTGAQGSGILRSMSEANCFVVLGHEQGAVKPGDAVDVMPMDGLV"
BASE COUNT          479 a         1191 c         1299 g          465 t
ORIGIN      
        1 tctcgaggcc gcatccgctt tccggagcgg gcgctgcgct tcgaatcgcc gatcggcgcg
       61 gtcgtcatcg gcacggccgg cgacgacgtg cacggcgccg acgccgcgct catcgtcgat
      121 ccgggcggca acgacgtgta cgagcgcgcg cccgtcacgg gaggaggcgt ctcggtgatc
      181 gtcgatctcg gcggggacga tcgctaccgc ggatccgacg tcgcgatcca cggcctgtcg
      241 gcgatcgtcg atctcgccgg cgacgaccgc tacgccatgg gcggcgctgg gatcgccgcg
      301 gcggtcgcgg gcgtctcgct cgtgctcgat ttcgccggcg acgacgtcta cgaggcgggc
      361 atcttcgcgc agggcgcggc ggtgttcgga ctgggcgcgc tcgtcgatct cggcggcaac
      421 gaccgctatc gcgtgcaggc ggcgggccag ggattcgcca tgaccggagg cgtcggcctg
      481 ctctgggacc gcggcggcga cgactcgtac tccgcggcgg gaatgcccga cgccttcgat
      541 cgcggcggcg gcacgagctg ggcgcagggc gtgggcttcg ggttcaggac cgcgctcggc
      601 ggcggcatcg ggatcctgcg cgacgaggcg ggcgacgacc gctacgaggc cgagatgttc
      661 gcgcagggca cgagctactg gtacgggctg gggctgctgt gggacggcgg cggacgtgac
      721 gtctaccgcg ccgtccggta cgcgcagggg aacggcgtgc accaggcggt cggcgtgctg
      781 cgcgacgaag gcggcgacga ccgctacgag atgaagatcg gcgtcggcca gggcatgggc
      841 ctcgatctcg ccgtcggcgt gctgttcgac gcggccgggg acgacttcta cggcgcgccg
      901 gcgtacgcgc agggcaacgc caccgcgaac gggatcggca tcgcgttcga cgcgggcggc
      961 gcggaccgct ggcacgcgag cgcggaccgg cgcgtctggg gcgtcgccga gccgctgcgc
     1021 ggattgccga gcgtcggcgt catcgtctac gacgcggcgg gctcgatctt cgatcgcgac
     1081 ggcaaggtcg tgccggcgcc ggccggcgcg acgctcgagg acccggtcga agcggcgccc
     1141 gcgccgccga cggcgagctg cccgctaacg gtggcggcgc cgcccgagga cgatcgtccg
     1201 ctcgcggaga tgctgcggcg gctcacgccc ggcgtcgccg cgaacgcgat cgatccgatg
     1261 caattcgcgc ggatccgggc gcgtctctcg cgcgggatcc aggcggcgat gggacagctg
     1321 ctgcctgccg cggacgactt cgacatcgcg tactccttcg ccgcggcgct gcgctgcacg
     1381 ctcgcgatgg cgcccgccgc cgacgcgccg gcgatgtggg acgagctcga ggaggcgctg
     1441 ctcgagagcc cgcggggtcc gttcaccggc gcggtcatcg gcgcgctgcg cgagcgtccg
     1501 ccgccggcgg cgcagatgcg gcgcatcctc gcgacgctcg agcgcgatcc ggcgtgtgga
     1561 gtgcgcgtcg tgacgctgca ggcgagagag gcggtggcgg acgaggcttc gcgcgcggcg
     1621 ctggtatcgg cggcgcagga cgcgctgcgc tcgccgtgct ggcaactgca gtccgcggcg
     1681 ctgggggtcc tgaggcgcag cggcgcggcg cccgcgccgg acgcgcggct gccgggcttc
     1741 ctgcgccgcg cgccttagcg cgcgcctcac gccggcgatt cggcgctgcg cagcggcagc
     1801 gtgccggtcg gcgcgacccg gttgcgcccc gcgttcttcg ccgcgtacag cgcttcgtcg
     1861 gcgtgcttca tccacgaatc catggtctcg ccgggacgca gctcgctcac gccgatgctt
     1921 acggtcaccc gcgcggcctg ccccggccgc gacgcctcga tcgacgcgcg caactgctcg
     1981 gcgacgacgg cggccgcgct ctcgcgcgtg tcgggcagca gcagcatgaa ctcctccccg
     2041 ccgatccgga acagcaggtc gagcttgcgc gagcgcttgc cgatcagctc gacgaccgcg
     2101 cgcagcacgt cgtcgcccgc ggcatggccg aagtcgtcgt tgatgtgctt gaagtggtcg
     2161 atgtcgacga gcagcagcga cgccggcgcc gaggtgcggc gctggcgctc gatcgcgtcg
     2221 gagaggcagc gctccatgtg gcgccggttg aacgcgccgg tgagcgggtc gacgttcgcc
     2281 tggtcgagca gccgccggtg caggtcgaag acgatgccga ggatcaggtt cgcgagcacg
     2341 acggtgagcg cgagggtggc cgagaagcgg atggtgtagt ccgggccgat gaagtgatag
     2401 acgagcgcgc tgatcaggac cagcatcagg aggttgcaca cgttcgccat gcgccgcgac
     2461 agcgagaacg tgaacagcag caccgaggga tagcaccaca gcgcaccgta gaacccctgg
     2521 atcttgagcg acagcccgat cgccgggacg agcggcacga gcaggaagat cagcgggatc
     2581 ggcggcggac gcttgaggta cacggcgatc gcgttgatcc cgaggatggc cacgccgccg
     2641 aggatcccga tcgcgagcgc agcgttgccc tggatgaacg cgttcacggc gaacggcacc
     2701 agcagaagga ccgccgcggt cgcgaggctg tacatgatgt gcgcgcgata cgcgcccagc
     2761 gggtcgcgac cgagctcgac cgggctcgcg ttcgccatgc gcctagacca gcccgtccat
     2821 cggcatcacg tcgaccgcgt cgccgggctt caccgcgccc tgctcgtggc cgagcacgac
     2881 gaagcagttc gcctcggaca tcgagcgcag gatgcccgag ccctgtgctc cggtgaggcg
     2941 caccgtccac tcgccgtcgc ggcgctcgag cacgccgcgc tggaactcgg tgcgcccggg
     3001 cttcttgcgc atcgcgacct ggctcttcgc gcgcagcagc ggcagctcgc agtcggcgca
     3061 tccggcgaga tgcagcagcg cgccgcgcac gaagtgatag aacgtgacca tcaccgcgac
     3121 cgggtttccc ggcagtccga agaggtaggc cgagcggccg cgcgcggcga tccgtccgaa
     3181 cgccatcggg cgtccgggcc gcatcgcgat cttccagaac acgacctcgc cgagcgccgc
     3241 catcatctgc ttggtgaagt cggcctcgcc gaccgacacg ccgcccgagg tgacgacggc
     3301 gtcggcgcga tcggccgcgt cgcggaacgc cgcttcgagc ttcgccgggt cgtcgcgcac
     3361 gacgccgagg tcgatcacct cgcagccgag gcgcgtcagc atgccccaga tcgtgtagcg
     3421 gttggagtcg taga
//
LOCUS       DATDMW010000090         4545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1394053, whole genome shotgun sequence.
ACCESSION   DATDMW010000090 DATDMW010000000
VERSION     DATDMW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394053"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..2492)
                     /locus_tag="VI319_03795"
     CDS             complement(102..2492)
                     /locus_tag="VI319_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6862998.1"
                     /translation="MSDFDDIARGLNFDGPNSYIGRSVTRPNARRLAQGRGQFVDDVM
                     LARTAHVAYARSPHAHARIAAIDKTKASAMPGVIRIATGAEIALVCKPYVGVLTHLAG
                     MRSPPQHPLAVDVARWQGEPVVAVVANTRAEAEDAAEAVDIEYEELPAALDAERALDP
                     DAVVIHREFGSNLCFQRTVDTGGVEAAMAAAHVVVEDTLVFARHTGVTNEPRSILADY
                     NRADETLTVYHSGQSPHMMQGIIASRLSLDEHRVRIVCRDVGGSFGIKIHTYGDEIAA
                     CALSLMLGRPVKFVADRLESFLADIHARDHRVRAKLGLDRDGNILALEIDDLTGIGPY
                     SMFPRSSGVECNQVLNITGGPYKHKEYRAKGTVVFQNKNLMCQYRAVGHPIATAVAEH
                     MVDQGAAALGIDPAEMRKRNLIPDDAYPYKSPSGMRFEALSHHQCLAKLLGIMDYEGL
                     RREQAALRKQGVHRGIGLASFIEVTTPSPMFYGVGGARISATDGCTIKLDANGAVVAA
                     TGVTEQGQGTETIVAQIAATAVGVEMSCVKVMTGDTDKTPYGGGTWASRAAGIGGEAV
                     LQAGLALKRNILEAGAVILKQRVEDLDIVDGKVVKKGSEQGMTLAELARICYYRGNEL
                     PVDFQPELVVTRHYRVKDYPFVFTNGIQGCSLEVDVDTGFVRLLKVWCVDDCGRVINP
                     KLVDEQVRGGIVQGIGGALYEHCLYDDRGQLQNATMADYLVPMAAEMPDIVTAHVQTP
                     TRTSVLGAKGAGEAGTGGAPAVVMNAVNDALRPLGAKVTSQPITPEAVLRALGKVR"
     gene            complement(2489..3007)
                     /locus_tag="VI319_03800"
     CDS             complement(2489..3007)
                     /locus_tag="VI319_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6862999.1"
                     /translation="MSAPAAVPVEVLEIALTVNGERVTRSVPVRLNLADFLRNELQLT
                     GTHVGCEHGVCGACTVRVDGEIARGCLMFAVQADGCRVDTIEGLSDSREIEELQSAFV
                     LTNALQCGFCTPGVLMAAQALLRDEPSPSRERIREYLSGNYCRCTGYQAIVDAVEAVG
                     KARASRRGSGGK"
     gene            complement(3004..3867)
                     /locus_tag="VI319_03805"
     CDS             complement(3004..3867)
                     /locus_tag="VI319_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY6863000.1"
                     /translation="MKAPRFAYARPSSVAETLALLAQHKGDARVLAGGQSLVPMLNFR
                     VAAPAVLVDINRVAGLSGIDVAKDRVRIGAMTRHTEIERSPAIARHLPLLAEAIAHVA
                     HPAVRNRGTFGGSCALADPAAELPACALALGGTFVVAGPRGERRVPAAEFFTGLYATA
                     LDPRELLVAAEFPLPAPGAVSAFGELARRHGDYALVGVAAHALARGGALHDARVVFFG
                     VGDKPVRAARIERALEGRAADARAIAGALEQLDADLAPRADLHASAPTKRHLAKVLAG
                     RVLARLPGAAA"
     gene            3914..4486
                     /locus_tag="VI319_03810"
     CDS             3914..4486
                     /locus_tag="VI319_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863001.1"
                     /translation="MSYRETLPRIRVSRADMMKRVARFSKLKGFDGGLPDSKMPGCER
                     TLYNVIGFQPPAVEREGMQSPVGEQAARMAAIKISEGFNLGYCKALPGRGPMLHNHDT
                     NETFIAITGTWRASWENEKGRLEHVDLKPLDVISFPAGASRRFMNVTKGPKNKPSILM
                     FIIGGDGPSAEFTSESMAALERAGIWPPKA"
BASE COUNT          641 a         1609 c         1609 g          686 t
ORIGIN      
        1 gaactcgcgc cgcgcctcgg gcgtgccgta gtattcgggc agctccgcgt ggggtcgcag
       61 ccccggaggc cggggttcgg tcgcgctgtt catcatcgct ctcatcttac cttcccgagc
      121 gcgcgcagca ccgcctcggg cgtgatcggc tggctcgtca ccttcgcgcc gagcggccgc
      181 agcgcgtcgt tcaccgcgtt catcacgacc gccggcgcgc cgccggtgcc ggcctcgccc
      241 gcgcctttcg cgccgagcac cgaggtgcgg gtcggcgtct gcacgtgcgc ggtgacgatg
      301 tcgggcatct ccgccgccat cggcacgagg tagtcggcca tggtcgcgtt ctgcagctgc
      361 ccgcgatcgt catagaggca gtgctcgtaa agcgcgccgc cgatgccctg cacgatcccg
      421 ccgcgcacct gctcgtcgac gagcttcggg ttgatgaccc gcccgcaatc atcgacgcac
      481 cagaccttca gcagcctgac gaagccggtg tcgacgtcga cctcgagcga gcagccctgg
      541 atgccgttgg tgaacacgaa aggataatcc ttcacccgat agtgacgggt cacaaccaat
      601 tccggctgga agtcgaccgg cagctcgttg ccgcggtagt agcagatgcg cgccagctcg
      661 gcgagcgtca tgccctgctc gctccccttc ttcacgacct tgccgtcgac gatgtcgaga
      721 tcctcgacgc gctgcttcag gatcaccgcg cccgcctcga ggatgttgcg cttgagcgcg
      781 aggccggcct ggagcacggc ctccccgccg atgcccgcgg cgcgcgacgc ccaggtgccg
      841 ccgccgtagg gcgtcttgtc ggtgtcgccg gtcatcacct tgacgcagct catctccacg
      901 ccgaccgcgg tcgccgcgat ctgggcgacg atggtctcgg tgccctggcc ctgctcggtg
      961 acgccggtcg cggcgacgac cgcgccgttc gcgtcgagct tgatggtgca gccgtcggtc
     1021 gccgagatgc gcgctccgcc cacgccgtag aacatggggc tcggagtggt gacctcgatg
     1081 aacgaggcga gcccgatgcc gcggtggacg ccttgcttgc gcagcgcggc ctgctcgcgc
     1141 cgcaggccct cgtagtccat gatcccgagc agcttcgcca ggcactggtg atgcgagagc
     1201 gcctcgaagc gcattcccga gggcgacttg tacgggtagg cgtcgtcggg gatgagattg
     1261 cgcttcctca tctccgccgg gtcgatgccg agcgccgccg cgccctggtc gaccatgtgc
     1321 tcggcgaccg cggtcgcgat cgggtggccg acggcccggt actggcacat caggttcttg
     1381 ttctggaaca cgaccgtgcc cttcgcccgg tactccttgt gcttgtacgg gcccccggtg
     1441 atgttcagca cctggttgca ctcgacgccg gatgagcgcg ggaacatcga gtacgggccg
     1501 atgccggtga ggtcgtcgat ctcgagcgcg aggatgttgc cgtcgcggtc gaggccgagc
     1561 ttcgcgcgca cgcggtggtc gcgtgcgtgg atgtccgcga ggaaggactc gagccggtcg
     1621 gcgacgaact tcaccggccg cccgagcatg agcgacagcg cgcacgccgc gatctcgtcg
     1681 ccgtaggtgt ggatcttgat gccgaacgag ccgccgacgt cgcggcacac gatgcgcacg
     1741 cggtgctcgt cgagcgacag ccggctcgcg atgatcccct gcatcatgtg cggcgactgc
     1801 cccgagtggt acaccgtgag cgtctcgtcg gcgcggttgt agtcggcgag gatggagcgc
     1861 ggctcgttgg tgacgccggt gtggcgggcg aagacgagcg tgtcctcgac caccacgtgc
     1921 gccgcggcca tcgccgcctc gacgccgccc gtgtcgacgg tgcgctggaa gcacaggttc
     1981 gagccgaatt cccggtggat gacgaccgcg tccgggtcga gcgcgcgctc ggcatcgagc
     2041 gcggcgggga gctcctcgta ctcgatatcc accgcctcgg ccgcgtcctc ggcctcggcg
     2101 cgcgtgttcg cgaccaccgc gacgacgggc tcgccctgcc agcgcgcgac gtcgaccgcg
     2161 agcggatgct gcggcggcga gcgcatgccg gcgaggtgcg tgagcacgcc gacgtaaggc
     2221 ttgcacacca gcgcgatctc ggcgccggtc gcgatccgga tcacccccgg catcgccgac
     2281 gccttcgtct tgtcgatcgc cgcgatccgc gcgtgcgcgt gcggcgagcg cgcgtaggcg
     2341 acgtgcgcgg tgcgcgccag catcacgtcg tcgacgaact gcccgcgccc ctgcgcgagg
     2401 cgccgcgcgt tcgggcgcgt caccgagcgg ccgatgtagc tgttcgggcc gtcgaagttc
     2461 agcccgcgcg cgatgtcgtc gaagtcgctc atttgccccc gcttcctcgt cgagatgcgc
     2521 gcgccttccc caccgcctcg accgcgtcga cgatcgcctg gtagccggtg cagcggcagt
     2581 agttgcccga gaggtattcg cggatgcgct cgcgcgatgg gctcggctcg tcgcgcagca
     2641 gcgcctgtgc cgccatcagc acgcccggcg tgcagaagcc gcattgcagc gcgttggtga
     2701 ggacgaaggc gctctgcagc tcctcgatct cgcgcgagtc ggacagcccc tcgatcgtat
     2761 ccacgcggca gccgtcggcc tgcaccgcga acatcaggca gccgcgcgcg atctcgccgt
     2821 cgacgcgcac ggtgcaggcg ccgcacacgc cgtgctcgca gccgacgtgg gtgccggtca
     2881 gctgcagctc gttcctgaga aagtcggcca ggttcaggcg caccgggacg ctgcgcgtca
     2941 cgcgctcgcc gttcacggtc agcgcaatct cgagcacctc aacgggcact gcggccggcg
     3001 cgctcatgcg gccgctccgg gcagtcgcgc gagcacgcgg cccgcgagca ccttcgccag
     3061 gtgacgcttg gtcggcgccg acgcgtgcag gtcggcgcgc ggcgcgaggt cggcgtcgag
     3121 ctgctcgagc gcgccggcga tcgcgcgcgc gtccgcggcg cgcccttcga gcgcgcgctc
     3181 gatcctcgcg gcgcgcaccg gcttgtcgcc gacgccgaag aacacgacgc gcgcgtcgtg
     3241 cagcgcgccg ccgcgcgcga gcgcgtgcgc ggcgacgccg acgagggcgt agtcgccgtg
     3301 ccggcgcgcg agctcgccga acgcggaaac ggcgcccggc gcgggcagcg gaaattccgc
     3361 cgcgacgagc agctcccggg gatcgagcgc ggtcgcgtac aggccggtga agaactcggc
     3421 cgccggcacg cggcgctcgc cgcgggggcc cgcgacgacg aaagtgcctc cgagcgcgag
     3481 cgcgcacgcg ggcagctcgg ccgccgggtc ggcgagcgcg cagctgccgc cgaaggtgcc
     3541 gcggttgcgc accgcgggat gcgcgacgtg cgcgatcgcc tcggcgagca gcggaaggtg
     3601 acgcgcgatc gcgggcgagc gctcgatctc ggtgtggcgc gtcatggcgc cgatgcgcac
     3661 acggtccttc gccacgtcga tccccgagag tcccgcgacg cggttgatgt cgaccaggac
     3721 cgcgggagcg gccacccgga aattgagcat cgggacgagg ctctgcccgc ccgcgagcac
     3781 gcgggcgtcg cccttgtgct gcgcgagcag cgccagggtc tcggcgacgc tcgagggtcg
     3841 cgcgtaggcg aatcgcgggg ctttcatgga tcgtccttca gtgacgtatt atgcccgcat
     3901 cgaggagacc atcatgagct accgggaaac gctgcctcgc atccgcgtct cgcgcgccga
     3961 catgatgaag cgcgtcgcgc gcttctcgaa gctgaagggc ttcgacggcg ggctgcccga
     4021 ctcgaagatg ccgggctgcg agcgcacgct ctacaacgtc atcggcttcc agccgcccgc
     4081 ggtcgagcgc gaaggcatgc agtcgcccgt cggcgagcag gccgcgcgca tggctgcgat
     4141 caagatctcc gagggcttca acctcggcta ctgcaaggcg ctgccgggca gggggccgat
     4201 gctgcacaac cacgacacca acgagacctt catcgcgatc accggcacct ggcgcgcgag
     4261 ctgggagaac gagaaaggca ggctcgagca cgtggacctg aagccgctcg acgtgatctc
     4321 gttcccggcg ggcgcgtcgc ggcgcttcat gaacgtcacg aaaggcccga agaacaagcc
     4381 gtcgattctc atgttcatca tcggcggcga cggtccaagc gccgagttca cgagcgagtc
     4441 gatggccgcg ctggagcgcg cgggcatctg gcccccgaaa gcgtagcggt ggacgtcggc
     4501 gtcttcgacc acctcgatct cggcgcgcag tcgcccgagc gctat
//
LOCUS       DATDMW010000091         7484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1021279, whole genome shotgun sequence.
ACCESSION   DATDMW010000091 DATDMW010000000
VERSION     DATDMW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021279"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3210
                     /gene="purL"
                     /locus_tag="VI319_03815"
                     /gene_synonym="purI"
     CDS             <1..3210
                     /gene="purL"
                     /locus_tag="VI319_03815"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY6863002.1"
                     /translation="GMIRATHARSPQGTVVAYSDNAAVMEGGAAASFHPDADGRYAYR
                     EQDVHTLMKCETHNHPTAISPFPGAATGSGGEIRDEGATGRGGKPKAGLCGFSVSHLR
                     IPGYEQPWERTVGKPGRIASALAIMLDGPIGAAAFNNEFGRPNLAGYFRTYEASVGGI
                     ERGYHKPIMIAGGIGSVTAENAKKAAVPAGALIVHLGGPGMLIGMGGGAASSMDTGAN
                     TEELDFDSVQRGNAEIERRAQEVIDRCWQLGAANPILSIHDVGAGGLSNAVPEIVHGA
                     GRGGRVDLRAVPNEEPGMTPREVWCNEAQERYVLAIAPESLDRFRALCERERCPFAVL
                     GAATNDGRLEVKDPRFDNAPVDMDLSVLLGKPPKMTRNVRRKRPELAALDLGDVDLRE
                     ACLRVLRAPTVANKTFLVAIGDRTVGGLCARDPFVGPWQVPVADCAVTLAGFRETRGE
                     AFAMGERTPIAVISPEASGRMAVGEALTNLAAAAVPDLGRVKLSANWMAAAGVEGEDA
                     ALFDTVRAVALELCPALGISIPVGKDSLSMRTTWREGGAEREVVAPLSLIVSAFAPCE
                     DARRTLTPQLRTDCGETVLILVDLGRGRNRLGASILAQVYGQTGDEAPDLDDPAALKN
                     AFAAIQALNRDGLVLAYHDRSDGGLFAAACEMAFAGHAGVTLNVDLLAYDALAHDVEG
                     AERRPEVLYGRDRERVLAALFAEELGMLIQVRAADRNRVLERLAGLDAQVVGAPNARD
                     EVRVVRNAKPLFAAPRAELERAWSEVTFRMQQLRDNPQCAREEHERLADASDPGLSAS
                     LSFDPAEDVAAPYIGRGARPRVAILREQGVNGQVEMAAAFDRAGFEAHDVHMSDILAA
                     RTPLAEFKGVVAGGGFSYGDVLGAGAGWAKAILFNARARDEFAAFFARRDSFALGVCN
                     GCQMMSNLRELVPGAQHWPRFLKNRSEQFEARFVMVEVTASPSLFFSGMAGSRMPIAT
                     AHGEGRAAFDSEEARARALVALRYVDNRGRPAELYPANPGGSAGGATGFTTEDGRFTI
                     LMPHPERVFRTVQHSWHPGNWGEDGPWMRMFRNARKAVA"
     gene            3289..3621
                     /locus_tag="VI319_03820"
     CDS             3289..3621
                     /locus_tag="VI319_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY6863003.1"
                     /translation="MLYRMSDLPSLLLFFVAAAAGNPSLHAAPARMQAVADTPALSAE
                     FAKLDADGDGTISRLEALKDPEVLRTFRRADANKDGRLDPREFATAQMLSRAERPASD
                     PKPRLATR"
     gene            complement(3629..4492)
                     /locus_tag="VI319_03825"
     CDS             complement(3629..4492)
                     /locus_tag="VI319_03825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6863004.1"
                     /translation="MLARLAVILSVALLPAAAWSQSDNGDAHPAPPPSPAAKAAAAKA
                     AAKAQAAKPVTVNGRPIPKARVDFFVKQQAAQGLPDNPQTRNLVIDNLISQEVVAQEA
                     EKKGFAKSSDFRTEMDLYRQRLLIRAFVQDHFKQHPIKDDDLLAEYNKVKASRGGDKE
                     YKARHILVDTESQAKDIVAQLKKGAKFEDLAKQSKDTGSKDKGGDLDWNTPGTFVKPF
                     ADAMTKLEKGKYTETPVQTQYGWHVIQLEDVREASFPAFNDVKPQIMARLQEQEVQKL
                     VSDLRAKAKIE"
     gene            complement(4543..4818)
                     /locus_tag="VI319_03830"
     CDS             complement(4543..4818)
                     /locus_tag="VI319_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY6863005.1"
                     /translation="MSAAIDLAATIRQRLAALEPAALELMDESGRHVGHEGAKSGGSH
                     FRLTIVSARFAGVATRERHRMVYAALGELMGREIHALAIAARAPDEL"
     gene            complement(4818..5420)
                     /locus_tag="VI319_03835"
     CDS             complement(4818..5420)
                     /locus_tag="VI319_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY6863006.1"
                     /translation="MKLLFDLFPVALFFIAFKFSDIYVATAVAIAASLAQIGWLKLRR
                     RKVDTMLWVSLAIIVVFGGATLLLRDETFIKWKPTVLYWSFAVALAASQLGFRRNLMR
                     TVLDEQVRMPEFAWSRLNWSWVGFFAFMGAANLYVAFSFSERYCSKVLDAAGRARCVD
                     DVWVNIKTIGGTGMLFAFIVLQAIYLSRYIEEKPKAGDAR"
     gene            complement(5477..5869)
                     /gene="msrB"
                     /locus_tag="VI319_03840"
     CDS             complement(5477..5869)
                     /gene="msrB"
                     /locus_tag="VI319_03840"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY6863007.1"
                     /translation="MAKVTKSDAEWRAQLTPMQYNVTRRKGTEHAFTGEYWDEKAAGT
                     YRCVCCGTPLFASDAKFDSGTGWPSYWQPVDPASVRTEEDSSWFMKRTEVLCANCDAH
                     LGHLFDDGPKPTGLRYCINSASLKFDKK"
     gene            5950..6714
                     /locus_tag="VI319_03845"
     CDS             5950..6714
                     /locus_tag="VI319_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6863008.1"
                     /translation="MKKTGSLVALAALAAAGCVSQGTFDEMQAAKNKEIQGLQSQRGT
                     LETQVQALQSQKADLERDKVSLSDQEADLRRQIQALEQQKAQLESASKQKASQYDSLI
                     QNLTEEVKKGQLQVRQYKDMLTVDVAEQLLFDSGRADLKESGKAVLKKVGEALKGYED
                     KVIRVVGHTDNIPITKGLQKRFASNWELSVARATTVVRFLQDVGVPPERMIASGRAEY
                     QPISENDTPEGRQKNRRTEITLVDRNAVTEATPPKQ"
     gene            complement(6732..>7484)
                     /locus_tag="VI319_03850"
     CDS             complement(6732..>7484)
                     /locus_tag="VI319_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6863009.1"
                     /translation="SQVGQAGKDVIWVPTPDEVVQRMLRMAQVTANDYVIDLGAGDGK
                     IAIAAAKKFGARSYGVEYNPDMAKFAQTNAQKAGVAGKATIINGDIFATDFSKATVIT
                     MYLLPSLNIKLRPTILQMRPGTRVVSHSFTMDDWMPDETSSISGRQAYFWIVPANVSG
                     KWSVELTGGASEKVDLVLDQRFQKIEGWAQLGQVQAGLRDPMLRGSEIYFMYVDPAGV
                     KRNFAGRVDGGHMEGSFRGDNGSEGRWTATRR"
BASE COUNT         1129 a         2562 c         2652 g         1141 t
ORIGIN      
        1 ggcatgatcc gcgccacgca cgcgcgcagc ccgcagggca ccgtggtcgc gtactccgac
       61 aacgccgcgg tgatggaggg cggagcggcc gcctcgttcc accccgacgc cgacggccgc
      121 tacgcgtacc gcgagcagga cgtgcacacg ctgatgaagt gcgagacgca caaccacccg
      181 accgcgatct cgccgttccc gggcgcggcg acggggtcgg gcggcgagat ccgcgacgaa
      241 ggcgcgaccg ggcgcggcgg caagccgaag gcgggcctgt gcggcttctc ggtgtcgcac
      301 ctgcgcatcc cgggatacga gcagccgtgg gagcgcacgg tcggcaagcc gggccgcatc
      361 gcctcggcgc tcgccatcat gctcgacggg ccgatcggcg cggcggcctt caacaacgag
      421 ttcgggcgcc ccaatctcgc cggctatttc cgcacctacg aggcgagcgt cggcggcatc
      481 gagcgcggct accacaagcc gatcatgatc gcgggcggga tcggcagcgt cacggcggag
      541 aacgccaaga aggccgcggt tccggccggc gcgctgatcg tccatctcgg cggccccggc
      601 atgctgatcg ggatgggtgg cggcgccgcc tcgagcatgg ataccggcgc gaacaccgag
      661 gagctcgact tcgactcggt tcagcgcggc aacgccgaga tcgagcggcg cgcgcaggag
      721 gtgatcgacc gctgctggca gctcggcgcg gcgaacccga tcctgtcgat ccacgacgtc
      781 ggggcgggcg ggctctcgaa cgccgtgccg gagatcgtcc acggcgcggg ccgcggcggc
      841 cgcgtcgacc tgcgcgcggt gccgaacgag gagcccggca tgacgccgcg cgaggtctgg
      901 tgcaacgagg cgcaggagcg ctacgtgctg gcgatcgcgc cggagtcgct cgaccggttt
      961 cgcgcgctct gcgagcgcga gcgctgcccg ttcgcggtcc tcggcgccgc gacgaacgac
     1021 gggcgactcg aggtgaagga cccgcgcttc gacaacgcgc cggtcgacat ggatctctcg
     1081 gtgctcctcg gcaagccgcc gaagatgacg cgcaacgtcc ggcgcaagcg cccggagctc
     1141 gccgcgctcg atctcggcga cgtcgacctg cgcgaggcgt gcctgcgcgt gctgcgcgcg
     1201 ccgacggtcg ccaacaagac cttcctcgtc gctatcggcg accgcacggt cggcggcctg
     1261 tgcgcgcgcg atcccttcgt cggcccgtgg caggtcccgg tcgccgactg cgcggtcacg
     1321 ctcgcgggct tccgcgagac gcgcggcgag gcgttcgcga tgggcgagcg cacgccgatc
     1381 gcggtgatct cgcccgaggc ctcggggcgg atggcggtgg gcgaggcgct gaccaacctc
     1441 gccgcggcgg cggttcccga tctcggccgc gtcaagctct cggccaactg gatggccgcc
     1501 gccggcgtcg aaggcgagga cgcggcgctt ttcgatacgg tgcgcgcggt cgccctcgag
     1561 ctgtgcccgg cgctcggcat ctcgatcccc gtcggcaagg attcgctatc gatgcgcacg
     1621 acctggcgcg agggcggagc cgagcgcgag gtggtcgcgc cgctgtcgct catcgtctcg
     1681 gcgttcgcgc cctgcgagga cgcgcgccgc acgctcacgc cgcagctgcg caccgattgc
     1741 ggcgagaccg tgctgatcct ggtggacctc ggccgcgggc gcaaccgcct gggcgcgtcg
     1801 atcctcgccc aggtctacgg ccagaccggc gacgaggcgc ccgacctgga cgatccggcc
     1861 gcgctcaaga acgccttcgc cgcgatccag gcgctcaacc gcgacggact ggtgctcgcg
     1921 taccacgacc gctccgacgg cggcctgttc gcggccgcgt gcgagatggc gttcgccggg
     1981 cacgccggag tcacgctcaa cgtcgacctg ctcgcctacg acgcgctcgc gcacgacgtc
     2041 gagggcgccg agcgccggcc cgaggtgctc tacggccggg accgcgagcg cgtgctggcg
     2101 gcgttgttcg ccgaggagct cgggatgctg atccaggtgc gcgccgcgga ccgcaaccgg
     2161 gtgctcgagc gcctcgcggg cctcgacgct caagtcgtcg gcgcgccgaa cgcgcgcgac
     2221 gaagtgcgcg tggtgcgcaa cgcgaagccg ctcttcgccg ccccgcgcgc cgagctcgag
     2281 cgggcgtggt ccgaggtcac cttccgcatg caacagctgc gcgacaaccc gcagtgcgcg
     2341 cgcgaggagc acgagcgcct cgccgacgcg tcggaccccg gcctctcggc gtcgctcagc
     2401 ttcgacccgg ccgaggacgt ggcggcgcct tatatcggcc gcggcgcgcg gccgcgggtc
     2461 gcgatcctgc gcgagcaggg cgtgaatggc caggtcgaga tggcggcggc gttcgaccgc
     2521 gcgggcttcg aggcgcacga cgtgcacatg agcgacatcc tcgccgcgcg cacgccgctc
     2581 gccgagttca agggcgtggt ggcgggcggc ggcttctcct acggcgacgt gctcggcgcg
     2641 ggcgccggct gggcgaaggc gatcctgttc aacgcgcgcg cgcgcgacga gttcgccgcc
     2701 tttttcgccc ggcgcgacag cttcgcgctc ggcgtgtgca acggctgcca gatgatgagc
     2761 aacctgcgcg agttggtgcc gggcgcgcag cactggccgc gctttctcaa gaaccgctcg
     2821 gagcagttcg aggcgcgctt cgtcatggtc gaggtgacgg cgagcccgtc gctgttcttc
     2881 tcggggatgg cgggcagccg catgccgatc gcgaccgcgc acggcgaagg gcgcgcggcc
     2941 ttcgactccg aggaggcccg cgcgcgcgcg ctggtcgcgc tgcgctatgt tgacaatcgc
     3001 gggcggcccg ccgagcttta tcccgcgaac ccgggcggat cggcgggcgg agccacgggg
     3061 ttcacgaccg aggacgggcg cttcaccatc ctcatgcccc atccggagcg cgtcttccgg
     3121 acggtgcagc actcgtggca cccgggaaac tggggcgagg acggtccgtg gatgcgcatg
     3181 ttccgtaacg cgcgcaaggc cgtcgcgtga caaagtgcac gatttgatgc gccgcacgac
     3241 ctagcgcagg gcggtgccgc ggcggcgctg ccgtcacacg cagccgacat gctgtatcgc
     3301 atgtcggacc tgccttcgct cctgctgttc ttcgtcgccg ccgcggccgg caacccgagt
     3361 ctgcacgccg cgccggcgcg gatgcaggcg gtcgccgaca cgccggcgct ctccgcggag
     3421 ttcgcgaagc tcgatgccga cggcgacggg accatatcgc gcctggaggc gctgaaggat
     3481 cccgaggtgc tgaggacttt ccgccgggcc gacgccaaca aggacggccg gctcgacccg
     3541 cgggagttcg cgaccgcgca gatgctctcg cgcgccgagc gcccggcctc ggaccccaag
     3601 ccgcgtctgg cgacgcgcta gagccgcctt actcgatctt cgccttcgcg cggagatcgc
     3661 tcaccagctt ctgcacttcc tgctcctgca gccgcgccat gatctgcggc ttgacgtcgt
     3721 tgaaggcggg gaacgacgcc tcgcgcacgt cctcgagctg gatcacgtgc cagccgtact
     3781 gggtctgcac cggggtctcg gtgtacttgc ccttctcgag cttcgtcatc gcgtcggcga
     3841 agggcttgac gaaggtcccg ggcgtgttcc agtcgaggtc gccgcccttg tccttggagc
     3901 ccgtgtcctt cgactgcttg gcgaggtcct cgaacttcgc gcctttcttg agctgcgcga
     3961 cgatgtcctt ggcctgcgac tcggtgtcga ccaggatgtg gcgcgccttg tactccttgt
     4021 cgccgccgcg ggaggccttg accttgttgt actcggcgag caggtcgtcg tccttgatcg
     4081 ggtgctgctt gaaatggtcc tggacgaagg cgcggatcag cagtcgctgg cgatagaggt
     4141 ccatctcggt cctgaagtcc gacgacttgg cgaagccctt cttctcggct tcctgcgcga
     4201 cgacctcctg gctgatcagg ttgtcgatca cgaggttgcg cgtctgaggg ttgtcgggca
     4261 ggccctgcgc ggcctgctgc ttgacgaaga agtcgacgcg cgccttcggg atcgggcggc
     4321 cgtttaccgt gaccggcttg gccgcttgag ccttcgcagc cgctttcgca gccgcggctt
     4381 tcgcggccgg cgatggaggc ggcgcgggat gcgcgtctcc gttgtccgac tgggaccagg
     4441 cggcggcggg aagcagcgcg accgacagga taacggcgag gcgagcgagc atagggattc
     4501 ctttggagaa agccggcgcg cggggcgcgg gaatgggccg aatcataact catcgggcgc
     4561 gcgggcggcg atggcgagcg cgtggatctc gcgtcccatc agctcgccga gcgccgcata
     4621 gaccatgcga tgacgctcgc gggtcgccac gcccgcgaat cgtgccgaca cgatcgtcag
     4681 gcggaaatgg ctgccgccgc ttttcgcgcc ctcgtggccg acgtggcggc ccgactcgtc
     4741 catgagctcc agcgcggccg gctcgagcgc ggcgagccgc tggcggatcg tcgcggcgag
     4801 atcgatggcg gcgctcatca ccgggcgtcg cccgccttgg gcttctcctc gatgtagcgg
     4861 gagagataga tcgcctggag aacgatgaaa gcgaacagca tcccggtgcc gccgatcgtc
     4921 ttgatgttca cccatacgtc gtcgacgcag cgggctcttc cggccgcgtc caataccttc
     4981 gagcaatagc gctccgagaa cgagaacgcg acgtacaggt tcgccgcgcc catgaacgcg
     5041 aagaaaccga cccagctcca gttgagccgg ctccaggcga attccggcat ccgaacctgc
     5101 tcgtccagca cggtgcgcat gagattgcgc cggaagccga gctgcgacgc cgcgagcgcg
     5161 acggcgaacg accagtacag gacggtcggc ttccacttga tgaaggtctc gtcgcggagc
     5221 agcagcgtcg ccccgccgaa tacgacgatg atcgccaggc tcacccagag catggtgtcg
     5281 accttgcgcc ggcgcagctt cagccagccg atctgcgcaa ggctcgcggc gatcgcgacc
     5341 gccgtcgcga cgtagatgtc ggagaacttg aacgcgatga agaagagggc gaccggaaag
     5401 aggtcgaaga gcagcttcat cccgcgatta taaccggccg cctcgggccg tccgcgccga
     5461 gcccgtcgag ccgcgcttac ttcttgtcga atttcagcga cgcggagttg atgcagtagc
     5521 gcaggcccgt gggcttcggc ccgtcgtcga agaggtgccc gaggtgcgcg tcgcagttcg
     5581 cgcacagcac ctcggtgcgc ttcatgaacc aggagctgtc ctcctcggtc ctcacgctcg
     5641 cggggtcgac gggctgccag tacgagggcc agccggtgcc cgagtcgaac ttcgcgtccg
     5701 acgcgaacag cggcgtgccg cagcagacgc agcggtacgt gcccgccgcc ttctcgtccc
     5761 agtactcgcc ggtgaaggca tgctcggtgc ccttcctgcg ggtgacgttg tactgcatgg
     5821 gcgtgagctg cgcgcgccac tcggcgtcgc tcttcgtgac tttggccatg ggtgtcgctc
     5881 ccgggagact cctatcgctg cggacgaata atatatagtg gaatctccac gtacgtctgg
     5941 agacaaccaa tgaagaagac cggatcgctt gtggcattgg ccgctctcgc ggccgcgggc
     6001 tgcgtcagcc agggaacgtt cgacgagatg caggcggcga agaacaagga gatccagggg
     6061 ctgcagtcgc agcgcggcac gctcgagacg caggtgcagg cgctgcagtc gcagaaggcc
     6121 gacctcgagc gcgacaaggt ctcgctctcc gatcaggagg cggacctgcg ccgccagatc
     6181 caggcgctcg agcagcagaa ggcgcagctc gagagcgcga gcaagcagaa ggcgtcgcag
     6241 tacgactcgc tgatccagaa cctcaccgag gaggtgaaga agggccagct gcaggtgcgc
     6301 cagtacaagg acatgctgac tgtcgatgtg gccgagcagc tgctgttcga ctcgggccgc
     6361 gccgatctca aggagtcggg caaggcggtg ctgaagaagg tcggcgaggc gctgaagggc
     6421 tacgaggaca aggtgatccg cgtcgtcggc cacaccgaca acatcccgat cacgaagggg
     6481 ctgcagaagc gcttcgcctc gaactgggag ctctcggtcg cgcgcgcgac gacggtcgtg
     6541 cgcttcctgc aggacgtggg cgtcccgccc gagcgcatga tcgcgtcggg acgcgccgag
     6601 taccagccga tcagcgagaa cgacacgccc gagggccggc agaagaaccg ccgcacggag
     6661 atcacgctgg tggaccgcaa cgcggtcacc gaggcgaccc ccccgaagca gtagcgccta
     6721 gacgatgtga gtcagcgccg cgtcgcagtc cagcggcctt cgctgccgtt gtcgccgcgg
     6781 aacgagccct ccatgtggcc gccgtcgacg cggccggcga agttgcgctt caccccggcc
     6841 gggtcgacgt acatgaagta gatctcggag ccgcgcagca tcgggtcgcg cagccccgcc
     6901 tgcacctggc cgagctgcgc ccacccctcg atcttctgga agcgctggtc gaggacgagg
     6961 tcgaccttct cgctcgcgcc cccggtcagc tcgacgctcc acttgccgct gacgttcgcc
     7021 gggacgatcc agaagtacgc ctggcggccc gagatgctcg aggtctcgtc gggcatccag
     7081 tcgtccatgg tgaacgagtg cgagaccacg cgcgtgccgg ggcgcatctg caggatcgtc
     7141 ggccgcagct tgatgttgag cgacggcagc aggtacatgg tgatgacggt cgccttgctg
     7201 aagtcggtcg cgaagatgtc gccgttgatg atcgtcgcct tgcccgcgac gcccgccttc
     7261 tgcgcgttgg tctgcgcgaa cttcgccatg tcggggttgt actcgacgcc gtaggagcgc
     7321 gcgccgaatt tcttcgccgc cgcgatcgcg atcttgccgt cgccggcgcc gaggtcgatc
     7381 acgtagtcgt tcgcggtgac ctgcgccatc cggagcatgc gctggaccac ttcgtcgggc
     7441 gtcggcaccc agatcacgtc cttgccggcc tgcccgacct gcga
//
LOCUS       DATDMW010000092        10221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1269977, whole genome shotgun sequence.
ACCESSION   DATDMW010000092 DATDMW010000000
VERSION     DATDMW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269977"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VI319_03855"
     CDS             <1..379
                     /locus_tag="VI319_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HEY6863010.1"
                     /translation="PLSMEKRWFANHVAYTIAKYGMSMCVLGMAEEFRADGVAVNALW
                     PRTVIQTAAIAMLGGLVKPENCRRPEIVADAAYIILNRDSRRHTGNFYVDDEVLAAEG
                     VTDLERYALVPGKARLLPDFFLD"
     gene            422..901
                     /locus_tag="VI319_03860"
     CDS             422..901
                     /locus_tag="VI319_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6863011.1"
                     /translation="MIKLTVNGKPRSADVEPEMPLLWVLRDVLDVKGPKFGCGIAQCG
                     ACTVHVNGEPVRSCSFPVSAAAGKRITTIEGLSANGKPHPVQAAWIEHQVPQCGYCQG
                     GQMLVAAALLKKNPNPSDKDIDEAMTNICRCGTYARMREAIHAAAKSGRGAKKPVKA"
     gene            912..3077
                     /locus_tag="VI319_03865"
     CDS             912..3077
                     /locus_tag="VI319_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6863012.1"
                     /translation="MTIKFASSRRTFLKQSAAVGGGLAVGFHVPSALAQGKGAEINGW
                     VVVRPDETIVIRYARAEMGQGSKTSAPMLVAEELECDWSKVRIEQVLASDNLRHKRAW
                     GDMASVGSRTIRNSQEYLRKAGATAREMLIAAAAQKWDVPAAECKASNGVVTHSSGRK
                     LSYGKLANDAAKIEPPKDVKLKDPKEWKIAGKPQKRVDIPDTAMGRQRYGIDVQMKGM
                     LYAAVAQSPVFGGKVRSMDASKAEGRRGVKKVVNLDTFVAVIADNWWRAKEALKLVQV
                     DWDTGNNGNVSSASLMDFYRAGLDATQDIAVARKVGDVDQALGSGGKVLEAEYFTPYL
                     AHSTLEPQNCTVLIKDGKVEVWVPTQNAEASLATAAATAGVPLDAVMVHPMQLGGGFG
                     RRGGGQDFVRIGVAVAKTMEGTPIKTLWSREEDTQHDLYRPASLVRMKGALDASGNPV
                     AWYSRVSSTSIITTLTKLPLKPPLAPAEGVDPQAVASIFDIPHAIPNIRVEYAQRNAH
                     VPVGFWRTVGHSQNPFVRESFMDELALAAGKDPFEFRRALLKDAPKDLGILEATAKAA
                     NWGSPLPAGVFRGIAETEAYGSYTSAVAELSVNDKGVVKIHRLVLGIDPGYAVNPDNV
                     VAQMQGSAVFMLTAMFWGEITIKDGRVEQSNFHDYRMMRLREMPKVEVVLAPSGGFWG
                     GVGEPGQASIAPAVCNAIYAATGKRIRTLPLKDQGISMA"
     gene            3074..3538
                     /gene="soxX"
                     /locus_tag="VI319_03870"
     CDS             3074..3538
                     /gene="soxX"
                     /locus_tag="VI319_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEY6863013.1"
                     /translation="MSKAKRLILGALAAFSFAAAPAQAEGLVKYKVEGDSIRAPLVRE
                     PGDAARGRKIVLDRSVGGCVLCHAFPEPEPRFMGDIGPPLAGVGARLSAGQLRLRVVD
                     QSRIDPRTPMPAYYRTEGLYDVATGYRGKPILDARQVEDVVAYLQTLREAKR"
     gene            3535..3990
                     /locus_tag="VI319_03875"
     CDS             3535..3990
                     /locus_tag="VI319_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEY6863014.1"
                     /translation="MKRREFIAAAASLALAPGALRAQDNPIAPIDLRDPDVQSVTKGA
                     PIRAGRVKLELPPLADNGNSVAMKLTVESPMTVQDHVRAVHLFSERNPVRHMAAFYLG
                     PKSGRAEIDSRIRLAGSQKIIALAEMSDGSFWAGSASVVVTLSACLDES"
     gene            3992..4309
                     /gene="soxZ"
                     /locus_tag="VI319_03880"
     CDS             3992..4309
                     /gene="soxZ"
                     /locus_tag="VI319_03880"
                     /inference="COORDINATES: protein motif:HMM:NF020350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEY6863015.1"
                     /translation="MAARIQVPPQAKRGEPMPVRIIIQHPMETGYRHDEVGHPIKRNV
                     IRELSCRYNGVEVFRADLSSGIAANPYIQFYTVAEASGVLEFSWIDDEGVRGSERAPV
                     VVA"
     gene            4306..5091
                     /gene="soxA"
                     /locus_tag="VI319_03885"
     CDS             4306..5091
                     /gene="soxA"
                     /locus_tag="VI319_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEY6863016.1"
                     /translation="MIALRAVAALAALALCAGATAQERALPLERLKPGTDFLGADLRA
                     LQSDDLANPAMLWVERGAKMWSERAGTAGRSCADCHQDAARTMRGVAASYPKFDAARG
                     HLFNLESRINACRVEHQGAQPFKHESDELLSITAYVASQSRGLPIHVSIDGPARAHFE
                     AGRAAYYRRRGQMNLACAQCHEENWGKRLFAETVSQGHPTAYPAYRMEWQTVGSEERR
                     LRACLSGVRAEMLPYGAPEYLDLELFLAWRAEGLKVESPGVRR"
     gene            5192..5797
                     /locus_tag="VI319_03890"
     CDS             5192..5797
                     /locus_tag="VI319_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6863017.1"
                     /translation="MAAAAFALASTAACAQSGWYGGVSLGQSKIQIDDSSVDVAGATA
                     SSVTKDETDTGFKLYAGNRLNRNFALEGGFTDFGKFSATRNVTAPAAGSATATVKSSG
                     FHFDALGILPVGGFDLFAKVGLIYTTTKTDLSTSGALVAVSANGKSSEFDGKYGIGAE
                     YRLPNAMGIRAEWERALNVGDPDKTGEGNIDLISVGLTWRF"
     gene            complement(5801..6799)
                     /locus_tag="VI319_03895"
     CDS             complement(5801..6799)
                     /locus_tag="VI319_03895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY6863018.1"
                     /translation="MTLRLGVLDQSPIVSGRSPAEAVAETLALARLAEDLGYGRYWLA
                     EHHAIAALADPCPEILLARLAAETSRMRVGTGGVLLPYYSPFKVAEIFRMLEALYPGR
                     IDLGIGRAPGGDLRTAQAVAYGAGISPEHFPRQVEDLAGFLDGTLPAGHPFAGLKAQP
                     AGATAPQIWLLGSSDYSGALASQLGLRFAFAHFISARGAGAVMRDYRAQYRPSAREPE
                     PASLLCVFVICAATGAEAERLAASIDLRRLHMARGIDAPVPTLEEAAAHRYTEQERAY
                     VQQQRARLVHGDPGAVREKLLALREETQADELMILSITGDYESRRESYRLVAKAML"
     gene            complement(6816..7007)
                     /locus_tag="VI319_03900"
     CDS             complement(6816..7007)
                     /locus_tag="VI319_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863019.1"
                     /translation="METAYKWMGIIFGIGILLIVVEINFARKKKEGFTPTDKKRVVGI
                     FWVSLFLAGLVGALIWASD"
     gene            7101..7889
                     /locus_tag="VI319_03905"
     CDS             7101..7889
                     /locus_tag="VI319_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY6863020.1"
                     /translation="MTAARLAGLCLLVAACAANAAAPQCSARSGPRTAALVELYTSEG
                     CDSCPPADRWLSSLFASGYTASQVVPLALHVDYWDYIGWKDPYAKRQFSARQHKLAKL
                     GRATFVYTPQVLLQGRDFRGWATDEFAKTVAQINARPARAALALTIRSIGPSGADVEL
                     GAELADAALRDETGLYVAAYENKLASDVRAGENRGRRLAHDFVVREWLGPVAFGADGR
                     IAQRRTLPLLPEADPAQSGVAAFVQDRRTGEVLQALMLPACGAH"
     gene            7915..8865
                     /locus_tag="VI319_03910"
     CDS             7915..8865
                     /locus_tag="VI319_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863021.1"
                     /translation="MKRWLGVAALCAAAAAHAQSWPSKPIRFVVGFPPGGGNDIIARL
                     LGAKMQENWGEPVVVENRPGAASIIAAELVAKSPADGYTLLVNATGGMSLNPILYPKL
                     PYDPLKDFEPISMVGVFPLVLVVNPSVPARSVRELVAHARKNPGKLNYSAGSTSFQVA
                     TEMFKQMTGTDIRHIPYKGSAASINAVLAGDVQLTIVDTPPLAGHLQSDRLRALAVTS
                     AKRAPGLPDLPTLAEAGVPGYDMSLWIGLFAPAGTPRAIVAKLSAEVARIVRLPDVRD
                     KLVGMGVDPLGNSPEQVADWIRAELARYRPVVRAANIKAE"
     gene            complement(8870..9643)
                     /locus_tag="VI319_03915"
     CDS             complement(8870..9643)
                     /locus_tag="VI319_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863022.1"
                     /translation="MRRHLALAAALLAASPSPALAAEGLTTLSVGADYTTGKYGATQS
                     TDILYIPFTGKYETGPWTAKLVVPWIHITGPGNVVGAPGDTVVISGASTGRRTESGLG
                     DVVASAFYNVLNERNAPVGLDLGGKVKFGTADENRGLGTGKNDYSLQADLFKPLGALT
                     PFGSIGYRKYGDPAGVDFKNVVYWSLGASYKMSQPTTVGVAYDFRPAIIDGGSHVSEG
                     TFFVSQKLNADWKVQLYAVKGFSTGSPDWGVGGVLAYSY"
     gene            9739..9885
                     /locus_tag="VI319_03920"
     CDS             9739..9885
                     /locus_tag="VI319_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863023.1"
                     /translation="MTIDRFRTLDALHAFYALDPLDALGPVRAFRTVRAIGPVGTHLV
                     GRSA"
BASE COUNT         1572 a         3564 c         3615 g         1470 t
ORIGIN      
        1 cccgctctcg atggagaagc gctggttcgc gaaccacgtc gcgtacacca tcgcgaagta
       61 cggcatgagc atgtgcgtgc tcggcatggc cgaggagttc cgcgccgacg gcgtcgcggt
      121 gaacgcgctg tggccgcgca ctgtgatcca gacggctgcg atcgccatgc tcggggggct
      181 ggtgaagccc gagaactgcc gccggccgga gatcgtcgcc gacgcggctt acattatcct
      241 gaaccgcgac agccgccggc acaccggtaa tttctatgtc gacgacgagg tgctggcggc
      301 cgagggggtg accgacctcg agcgctacgc gctcgtgccc ggaaaggccc ggctgctgcc
      361 cgacttcttt ctggattaat atccggaagc tcactcaatc cagcctcgag gaggatctcc
      421 catgatcaaa ctgaccgtca acggcaagcc gcgctcggcc gacgtcgagc cggagatgcc
      481 gctgctgtgg gtgctgcgcg acgtgctcga cgtcaagggc cccaagttcg gttgcggtat
      541 cgcgcaatgc ggagcgtgca ccgtgcacgt gaacggcgag ccggtgcgct cgtgctcgtt
      601 cccggtctcg gccgcggcgg gcaagcgcat cacgacgatc gagggcctgt cggcgaacgg
      661 caagccgcac cccgtgcagg ccgcgtggat cgagcaccag gtgccccagt gcggctactg
      721 ccagggcggc cagatgctgg tcgccgcggc gctgctcaag aagaacccga accccagcga
      781 caaggacatc gacgaagcga tgaccaacat ctgccgctgc ggcacgtacg cgcgcatgcg
      841 cgaggcgatc cacgccgcgg cgaaatccgg gcgcggcgcg aagaagccgg tgaaggcgta
      901 aggagcagac catgaccatc aagttcgctt cttcccgcag gacgttcctc aagcagtcgg
      961 ccgccgtcgg cggcgggctc gcggtcggct tccacgtgcc gtcggcgctc gcgcagggca
     1021 aaggcgccga gatcaacggc tgggtcgtgg tgcggcccga cgagacgatc gtcatccgct
     1081 acgcccgcgc cgagatgggg cagggcagca agacctcggc gccgatgctg gtcgccgagg
     1141 agctcgagtg cgactggagc aaggtgcgca tcgagcaggt cctcgcgagc gacaacctgc
     1201 gccacaagcg cgcctggggc gacatggcct cggtcggctc gcgcaccatc cgcaactcgc
     1261 aggagtacct gcgcaaggcg ggcgccaccg cgcgcgagat gctgatcgcc gccgcggcgc
     1321 agaaatggga cgtgcccgcc gccgagtgca aggcgtcgaa cggcgtggtg acgcactcga
     1381 gcggccgcaa gctctcctac ggcaagctcg cgaacgacgc ggcgaagatc gagccgccga
     1441 aggacgtgaa gctgaaggac ccgaaggagt ggaagatcgc cggcaagccg cagaagcgcg
     1501 tcgacatccc cgacacggcg atgggcaggc agcgctacgg catcgacgtg cagatgaagg
     1561 gcatgctcta cgccgcggtc gcgcagtccc cggtgttcgg cggcaaggtc cgctcgatgg
     1621 acgcctcgaa ggccgaaggc cgccgcggcg tgaagaaggt ggtcaacctc gacaccttcg
     1681 tcgcggtgat cgccgacaac tggtggcgcg cgaaggaagc gctcaagctc gtccaggtcg
     1741 actgggacac cggcaacaac ggcaacgtct cgagcgcgag cctgatggac ttctaccgcg
     1801 ccggcctcga cgcgacgcag gacatcgccg tcgcgcgcaa ggtcggcgac gtcgaccagg
     1861 cgctcggctc cggcggcaag gtgctcgagg ccgagtactt cacgccctac ctcgcccact
     1921 cgacgctcga gccgcagaac tgcacggtgc tgatcaagga cggcaaggtc gaggtctggg
     1981 tgccgacgca gaacgccgag gcgtccctcg ccacggcggc cgcgaccgcg ggcgtcccgc
     2041 tcgacgcggt catggtgcac ccgatgcagc tcggcggcgg cttcggccgg cgcggcggcg
     2101 gtcaggactt cgtgcgcatc ggcgtcgccg tggcgaagac catggaaggc acgccgatca
     2161 agacgctgtg gagccgcgag gaggacacgc agcacgacct ctatcgcccc gcgagcctgg
     2221 tgcgcatgaa gggcgcgctc gacgcgagcg gcaacccggt cgcgtggtac tcgcgcgtgt
     2281 cgtcgacctc gatcatcacg acgctgacga agctgccgct caagccgccg ctcgcgcccg
     2341 ccgagggcgt ggacccgcag gcggtggcgt cgatcttcga catcccgcac gcgatcccga
     2401 acatccgcgt cgagtacgcg cagcgcaatg cgcacgtgcc ggtcggcttc tggcgcacgg
     2461 tggggcactc gcagaacccg ttcgttcgcg agtcgttcat ggacgagctc gcgctcgcgg
     2521 cgggcaagga cccgttcgag ttccgccgcg cgctgctcaa ggacgcgccg aaggacctcg
     2581 gcatcctcga ggcgaccgcg aaggccgcga actggggcag cccgctgccc gcgggcgtgt
     2641 tccgcggcat cgccgagacc gaggcctacg gcagctacac gtcggccgtg gccgagcttt
     2701 cggtgaacga caagggcgtg gtgaagatcc accggctggt gctcggcatc gacccgggct
     2761 acgcggtgaa ccccgacaac gtcgtcgcgc agatgcaggg ctcggccgtg ttcatgctga
     2821 ccgcgatgtt ctggggcgag atcaccatca aggacgggcg cgtcgagcag tcgaactttc
     2881 acgactaccg catgatgcgg ctgcgcgaga tgccgaaggt cgaggtggtc ctggcgcctt
     2941 cgggcgggtt ctggggcggc gtcggcgagc ccggccaggc gtcgatcgcg cccgccgtgt
     3001 gcaatgcgat ctacgccgcg accggcaagc gcatccggac gctcccgctc aaggaccaag
     3061 ggatctcgat ggcttgagca aggcgaagcg gttgattctc ggggcgctcg ccgctttttc
     3121 gttcgccgcg gcgcccgcgc aggccgaagg cctggtgaag tacaaggtcg agggagactc
     3181 gatccgcgcg ccgctcgtcc gggaaccggg cgacgcggcg cgcggtcgga aaatcgtgct
     3241 cgaccgctcg gtcggcggct gcgtgctctg ccacgcgttc ccggagcccg agccgcgctt
     3301 catgggcgac atcggcccgc cgctcgcggg cgtcggcgcg cggctttccg cggggcagtt
     3361 gcgcctgcgc gtggtcgacc agagccggat cgatccgagg acgcccatgc ccgcgtacta
     3421 ccgcaccgaa ggcctctacg acgtcgcgac cggctaccgc ggcaagccga tcctcgacgc
     3481 gcggcaggtc gaggacgtcg tcgcctacct gcagacgctg cgcgaggcga agcggtgaag
     3541 cgccgcgagt tcatcgccgc cgcggcctcg ctcgcgctcg cgcccggcgc gctgcgcgcg
     3601 caggacaacc cgatcgcgcc gatcgacctc agggaccccg acgtccagtc ggtcacgaaa
     3661 ggcgccccga tccgcgccgg gcgggtgaag ctcgagctgc cgccgctcgc cgacaacggc
     3721 aactcggtgg cgatgaagct cacggtcgag agccccatga cggtccagga ccacgtgcgc
     3781 gcggtgcacc tgttctccga gcgcaacccg gtgcggcaca tggcggcttt ctatctcggg
     3841 ccgaaaagcg ggcgcgccga gatcgactcg cgcatacgcc tcgcgggaag ccagaagatc
     3901 atcgcgctcg cggagatgtc cgacggctcg ttctgggccg gatcggcgtc ggtggtggtg
     3961 acgctctccg cctgcctgga cgagagctga gatggccgcg cgcatccagg tgcccccgca
     4021 ggcgaagcgc ggcgagccga tgccggtgcg gatcatcatc cagcacccga tggagaccgg
     4081 ctaccgccac gacgaggtcg gccacccgat caagcgcaac gtgatccgcg agctctcgtg
     4141 ccgctacaac ggcgtcgagg tgttccgcgc cgacctgtcc tcggggatcg cggccaatcc
     4201 ctacatccag ttctacaccg tcgccgaggc gagcggcgtg ctcgagttct cgtggatcga
     4261 cgacgagggc gtgcgcggct cggagcgcgc gccggtcgtg gtcgcatgat cgcgctgcgc
     4321 gcggtcgcgg cgctcgccgc gctcgcgctg tgcgccgggg cgacggcgca ggagcgcgcg
     4381 ctcccgctcg agcggctcaa gcccggcacc gacttcctcg gcgccgacct gcgcgcgctg
     4441 cagtccgacg atctcgccaa tcccgcgatg ctctgggtcg agcgcggcgc gaagatgtgg
     4501 agcgagcgcg cggggaccgc gggacgctcg tgcgccgact gccaccagga cgccgcgcgg
     4561 acgatgcgcg gcgtcgccgc ctcgtacccg aagttcgacg cggcgcgggg gcacctcttc
     4621 aacctcgaga gccgcatcaa cgcctgccgc gtcgagcacc agggcgcgca gccgttcaag
     4681 cacgagtccg acgagctgct gtcgatcacg gcctacgtcg cgagccagtc gcgcgggctt
     4741 ccgatccacg tcagcatcga cgggcccgcg cgcgcgcact tcgaggcggg ccgcgccgcg
     4801 tactaccggc gccgcggcca gatgaacctc gcctgcgcgc agtgccacga ggagaactgg
     4861 ggcaagcgcc tcttcgccga gaccgtgagc cagggccacc cgaccgccta cccggcgtac
     4921 cgcatggaat ggcagaccgt cgggtcggaa gagcgccgcc tgcgcgcctg cctctcgggc
     4981 gtgcgcgccg agatgctgcc ctacggcgcg cccgagtacc tcgacctcga gctcttcctc
     5041 gcgtggcggg cggagggact gaaggtcgag tcgccggggg tgcggcgcta ggactccgtt
     5101 gaacgttcgg cccgccgacc cggtcgaagt gtcggttcag ggaggccaac gaacaacgga
     5161 gggtccgatg agaagcatac tcacgagggc attggccgcg gctgcattcg cgctcgcgtc
     5221 gaccgcggcg tgcgctcaat ccgggtggta cggcggcgta tcgctagggc aatcgaagat
     5281 ccagatcgac gacagctcgg tcgatgtcgc cggagcgacg gcgagcagcg tgaccaagga
     5341 cgaaaccgac acgggcttca agctctacgc tggcaacagg ctcaaccgca acttcgcgct
     5401 cgagggcggt ttcaccgatt tcggcaagtt ctcggccacg cgcaacgtca ccgcgcctgc
     5461 tgccggctcg gccaccgcga cggttaaatc gagcggcttc catttcgacg cgctcgggat
     5521 cctgccggtc ggcggtttcg accttttcgc aaaggtcggc ctcatctaca cgacgaccaa
     5581 gaccgacctc tcgacctcgg gagcgctggt ggccgtctct gcaaacggca agagttccga
     5641 gttcgatggc aaatacggta tcggcgcgga gtatcgcctg ccgaacgcca tgggcatccg
     5701 agccgagtgg gagcgtgccc tcaacgtcgg ggacccggac aagaccggcg aaggcaacat
     5761 cgacctgatc tccgtcggcc tcacctggcg gttctagcgg tcagagcatc gctttcgcca
     5821 cgagccggta cgactcgcgc cggctctcgt agtccccggt gatcgacagg atcatgagct
     5881 cgtcggcctg cgtctcctcg cgcagcgcga ggagcttctc gcgcaccgcg cccgggtcgc
     5941 cgtgcacgag gcgcgcgcgc tgctgctgca cgtaggcgcg ctcctgctcg gtgtagcggt
     6001 gcgcggcggc ctcctcgagg gtcggcaccg gcgcgtcgat gccgcgcgcc atgtgcagcc
     6061 ggcgcaggtc gatgctcgcc gcgaggcgct cggcctcggc gccggtcgcg gcgcagatca
     6121 cgaagacgca cagcagcgac gcgggctccg gctcgcgcgc cgacggccgg tactgcgcgc
     6181 ggtagtcgcg catcaccgcg cccgcgccgc gcgcgctgat gaagtgcgcg aacgcgaagc
     6241 gcagcccgag ctgcgacgcg agggcgccgc tgtagtcgct cgagccgagc agccagatct
     6301 ggggcgcggt cgcgcccgca ggctgcgcct tcaggccggc gaaaggatgg cccgcgggca
     6361 gcgtgccgtc gaggaagccg gcgaggtcct cgacctgccg cgggaaatgc tcgggcgaga
     6421 tcccggcgcc gtaggccacc gcctgcgcgg tgcgcaggtc gcctccgggg gcgcgcccga
     6481 tgccgaggtc gatgcgcccc gggtagagcg cctcgagcat gcggaaaatt tccgcgacct
     6541 tgaaggggct gtagtagggc agcagcacgc cgcccgtgcc cacgcgcatg cgcgaggtct
     6601 ccgcggcgag ccgggcgagc aggatctcgg gacaggggtc ggcgagcgcg gcgatcgcgt
     6661 ggtgctcggc gagccagtag cggccgtatc cgaggtcctc ggcgaggcgc gcgagcgcga
     6721 gcgtctcggc gacggcctcg gccgggctgc ggccgctcac gatgggcgac tggtccagga
     6781 ccccgagacg cagggtcatc gccgcgcctc gatcttcagt cgctcgccca gatcagggcg
     6841 ccgaccagcc ccgccaggaa cagcgacacc cagaagatcc cgaccacgcg cttcttgtcg
     6901 gtgggcgtga agccctcttt tttcttgcgc gcgaagttga tctcgaccac gatcagcaga
     6961 atcccgattc cgaagatgat tcccatccat ttgtacgcgg tttccacgag cgggctccct
     7021 gggccggaaa gccgcagttt accgcgcggt tcatcggagc gcgccgtcgc ggcgggcgcg
     7081 gtgcgggtat catcgcggtc atgactgccg cgaggctcgc cggactctgc ctcctcgtcg
     7141 ccgcgtgcgc cgcgaacgcc gccgcgccgc agtgctcggc gcgcagcggg ccgcgcaccg
     7201 cggcgctggt cgagctctac acttccgagg gctgcgacag ctgtccgccc gcggaccggt
     7261 ggctgtcgtc gctgttcgcg agcggctaca ccgctagcca ggtcgtgccg ctcgcgctgc
     7321 acgtcgatta ctgggactac atcggctgga aggacccgta cgccaagcgc cagttctcgg
     7381 cgcgccagca caagctcgcg aagctcggcc gcgccacttt cgtatacacg ccgcaggtgc
     7441 tgctgcaggg acgcgacttc cgcggctggg cgacggacga gttcgcgaag acggtcgcgc
     7501 agatcaacgc gcgcccggcg cgcgccgcgc tcgccctcac gatccgctcc atcggtccgt
     7561 cgggagccga cgtcgagctc ggtgccgagc tcgccgacgc ggcgctccgc gacgagacgg
     7621 gcctctacgt cgccgcgtac gagaacaagc tggcgagcga cgtgcgtgcc ggcgagaacc
     7681 gcggccggcg cctcgcgcac gacttcgtcg tgcgcgagtg gctcggcccg gtcgccttcg
     7741 gcgcggacgg ccgcatcgcg cagcggcgca cgctgcccct gctccccgag gcggacccgg
     7801 cgcaatccgg cgtcgccgcg ttcgtgcagg accgccgcac cggcgaggtg ctgcaggcgc
     7861 tgatgctgcc cgcgtgcggc gcgcattgac tacaatccgc gcatgatccg cctcgtgaaa
     7921 aggtggctcg gcgtggccgc gctgtgcgcg gccgccgcgg cgcacgctca atcgtggccc
     7981 tcgaagccga tccgcttcgt ggtcggcttt ccgccggggg gcggcaacga catcatcgcg
     8041 cgcctgctcg gcgcgaagat gcaggagaac tggggcgagc ccgtcgtggt cgagaaccgc
     8101 ccgggggcgg cgtcgatcat cgcggccgag ctggtcgcga agtcgcccgc cgacggctac
     8161 acgctgctcg tcaacgcgac cggcggcatg tcgctcaacc cgatcctgta tccgaagctg
     8221 ccgtacgatc cgctcaagga cttcgagccg atcagcatgg tgggcgtgtt cccgctcgtg
     8281 ctggtggtga atccgtcggt gcccgcgcgc tcggtgcgcg agctggtcgc gcacgcgcgc
     8341 aagaaccccg gcaagctgaa ctacagcgcg gggtccacgt cgttccaggt ggcgaccgag
     8401 atgttcaagc agatgaccgg caccgacatc cggcacatcc cgtacaaggg cagcgcggcg
     8461 tcgatcaacg ccgtgctcgc gggcgacgtg cagctcacca tcgtcgacac gccgccgctc
     8521 gccggccacc tgcagtcgga ccgtctgcgc gcgctcgcgg tgacgtcggc gaagcgcgcg
     8581 ccgggattgc cggacctgcc gacgctcgcg gaggcgggcg ttccgggcta cgacatgtcg
     8641 ctgtggatcg gcctgttcgc gccggcgggg acgccgcgcg cgatcgtcgc gaagctctcg
     8701 gcggaggtcg cgcgcatcgt gcgcctgccc gacgtgcgcg acaagctcgt cggcatgggc
     8761 gtcgacccgc tcggaaacag ccccgagcag gtggccgact ggatccgcgc ggagctcgcg
     8821 cgataccgcc cggtggtgcg ggcggcgaac atcaaggccg agtgacccgc tagtagctgt
     8881 acgcgagcac cccgcccacg ccccagtccg ggctgcccgt cgagaagccc tttacggcgt
     8941 agagctggac cttccagtcg gcgttgagct tctgcgagac gaagaacgtg ccctcgctca
     9001 cgtggctgcc cccgtcgatg atggccgggc ggaaatcata ggcgacgccc acggtcgtcg
     9061 gctgcgacat cttgtacgac gcgccgagcg accagtagac cacgttcttg aagtcgacgc
     9121 cggccggatc gccgtacttg cggtagccga tcgagccgaa aggcgtcagc gcgccgagcg
     9181 gcttgaacaa gtcggcctgc agcgagtagt cgttcttgcc ggtgccgagg ccgcggttct
     9241 cgtcggccgt tccgaacttc accttgccgc cgaggtcgag cccgaccggc gcgttgcgct
     9301 cgttcagcac gttgtagaag gcgctcgcga cgacgtcgcc gagacccgac tcggtgcgcc
     9361 ggccggtgct ggcgccgctg atcacgacgg tgtcgccggg cgctccgacg acgttgcccg
     9421 gccccgtgat gtggatccag ggcacgacga gcttcgcggt ccacgggccg gtctcgtatt
     9481 tgcccgtgaa cggtatgtac aggatgtccg tgctctgggt ggcgccgtac ttcccggtcg
     9541 tgtagtcggc gcccacgctg agcgtggtca gtccttcggc cgcgagcgcg ggcgaggggg
     9601 aggccgcgag cagcgccgcg gcgagtgcga gatgtcgtct catgttcgtc ttctttcctg
     9661 gtgggggtta gggattttgg ccggtcggcc ggctcttagt cagtgatgtc cgctacattc
     9721 gggcgttcag cgtccgccgt gacgatcgat cgtttccgga cgctcgacgc gctccacgcg
     9781 ttctacgcgc tcgacccgct cgacgcgctc gggccggtcc gcgcgttccg gacggtccgc
     9841 gcgatcgggc cggtcggcac gcaccttgtt ggtcgaagtg cctgacgccg atccattggg
     9901 catcgcggcg cggttcactt gattcggcgc cccgccaccc gatgatgcgg agccggccga
     9961 tgacacgggg gccgccgatg acacgggggc cgccgatgat gccgcgccgc tcgatagcgc
    10021 cggaccaccc gatggcccgg agccgcccga tgatccggag ccgcccgatg atccggaact
    10081 ccccgacggt ccggggccac ccgaggatcc ggatccaccc gaggatccgg atccactcga
    10141 cggtccggga ccgccgcggc cgctcgaatc atcgcgcgga ccattgcccg ggccgccgtg
    10201 gccggaattg tccgaccgcc c
//
LOCUS       DATDMW010000093         2567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2392033, whole genome shotgun sequence.
ACCESSION   DATDMW010000093 DATDMW010000000
VERSION     DATDMW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392033"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1441
                     /locus_tag="VI319_03925"
     CDS             68..1441
                     /locus_tag="VI319_03925"
                     /inference="COORDINATES: protein motif:HMM:NF007135.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HEY6863024.1"
                     /translation="MTARVLVVDDVDANVKLLEARLTADYFEVRTARSGPEALHICAR
                     ERADVILLDVMMPGMDGFEVCRRLKAETRTQHIPVIMVTALDQPSDRVKGLEAGADDF
                     LTKPVDDIALITRVKNLARLKMLTDEMLMRASTEEQMGFAATMGIRLDQLGRGGRILL
                     VEDRDRSADRIIETLGAEHHIEREADPARALLRLPEGNFDLMIVSLSLEHADGLRLCS
                     QVRSLDRTRHLPIMMIIEPGDDARLLRGLDMGVNDYIVRPVDPNELLARVRTQVKRKR
                     YTDHLRTRLEETVEMAILDPLTALHNRRYMTSHLKTLFEESSQRGKPLSVLLLDIDYF
                     KAVNDSHGHDAGDSVLREFASRVRRNIRGIDLACRLGGEEFVVVMPDTDLAKAYLVGE
                     RLRQCIAAAPFYAGERIGTLEVTASVGVAALEFADDTPELILKRADQALYCAKRDGRN
                     RVVADAA"
     gene            complement(1929..2096)
                     /gene="rpmG"
                     /locus_tag="VI319_03930"
     CDS             complement(1929..2096)
                     /gene="rpmG"
                     /locus_tag="VI319_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY6863025.1"
                     /translation="MAKPVTQKIKLVSSAGTGYYYVTKKNPRNQTEKLVFKKYDPVAR
                     KHVEFKEAKIK"
     gene            complement(2153..>2567)
                     /locus_tag="VI319_03935"
     CDS             complement(2153..>2567)
                     /locus_tag="VI319_03935"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY6863026.1"
                     /translation="LRGAKHKCPACGVGALYWRFLKVSDTCPHCGEELHHHRADDAPP
                     YFTVVIVGHIVVALVLLVEMTYRPPLWVHAALWGPLTIILALILLPSVKGGLIGLQWA
                     LRMHGFDPNATEDGIERFVVPEDEQVVVPARLPRS"
BASE COUNT          488 a          769 c          805 g          505 t
ORIGIN      
        1 cggtcgccat gttcctcgat actgtccgcc agttcatcgg cgtcgcacga taacacggta
       61 acaagttatg accgcccgcg tgcttgtggt tgacgacgtc gacgccaacg tgaagctgct
      121 cgaggcgcgg ctgacggccg actatttcga ggtgcggacc gcgcggagcg gtccggaggc
      181 attgcatatt tgcgcgcgcg aacgcgccga cgtcatcctg ctcgacgtga tgatgccggg
      241 catggacggc ttcgaggtct gccgaaggtt gaaagcggag acccgcactc agcacatccc
      301 ggtcatcatg gtcaccgccc tcgatcagcc gtcagaccgc gtgaaggggc tcgaggcggg
      361 agccgacgac ttcctcacca agccggtcga cgatatcgcg ctcatcacgc gagtaaagaa
      421 tctcgcgcgt ctcaagatgc tgaccgacga gatgctgatg cgcgcctcga ccgaggagca
      481 gatgggtttt gccgcgacta tgggcatcag gctcgatcag ctcgggcgcg gcgggcgcat
      541 tcttctcgtc gaggaccgtg accgctcggc cgaccgcatc atcgagacgc tgggcgccga
      601 acatcatatc gagcgtgagg cggacccggc gcgggctctg ctgcgtctcc ccgagggcaa
      661 tttcgatctc atgatcgtca gtctcagcct cgagcatgcc gatggcttga ggctgtgctc
      721 acaggttcgc tcgctcgatc gcacgcgcca tctgccgatc atgatgatca tcgagccagg
      781 cgacgacgcg cgcctgttgc ggggtctcga catgggggtc aacgactata tcgtccgccc
      841 ggtggacccc aacgagctgc tcgcgcgcgt gcgcacgcag gtgaagcgca agcgctacac
      901 cgatcatttg cgcaccaggc tcgaggaaac cgtcgaaatg gcgattctcg acccgctcac
      961 cgcgctgcac aaccggcgct acatgacgag ccatttgaag accttgttcg aggagtcctc
     1021 gcaacggggc aaaccgcttt cggtgctgtt gctcgacatc gactatttca aggcggtgaa
     1081 cgacagccac ggccatgacg cgggagactc ggtgctgcgc gagtttgctt cccgcgtccg
     1141 tcgcaatatt cgcggcatcg acctcgcgtg ccggctgggc ggagaggaat tcgtggtggt
     1201 gatgccggat accgacctcg ccaaggccta tctggtcggc gagcgcctgc gccagtgcat
     1261 tgccgcggct ccgttctatg ccggggagag gatcgggact ctggaagtga cggcaagcgt
     1321 cggtgtggcg gcgctcgaat tcgccgacga cacccccgag cttatcttga agcgtgccga
     1381 tcaggcgctc tattgcgcga agcgcgacgg gcgcaaccgg gttgtagccg acgccgctta
     1441 gcagctagcc agcgagcgct gcgttgggcc gaaagcgaat cattccaggc aaattcgttg
     1501 gctgagggcc tcttttgggc ctggtcggac ttgacgttgg acctgagcgc ttaaccttaa
     1561 ggctttacac tgcctagcat tttatcgtat taagccaaga aagcgggttg atttcgttcc
     1621 gtctttggct gttttatggt ttgactgtcc agctaagaca ggacgtggag gcaatcgggg
     1681 tgagggatct tgcgtgagcc aaagcccacg cgttcgcaac tcggttcctt cgaagcagag
     1741 ataaccctgc ggaatgctca ggtccgccgc atctcctggg tccgtagggt tcggccggcc
     1801 ggaccgcggt tgagtggcct cctcgttagc gccgcgtttc ggccggccac ctaattttgg
     1861 atgcaagcgc gcatgggccg cgcgtccaac gcgtgcgtcg ccaatcggcc caaagctcaa
     1921 gccaatcgct atttgatctt ggcttctttg aactcgacgt gcttgcgcgc gaccggatcg
     1981 tattttttga acacgagctt ctcggtctga ttgcgcgggt tcttcttggt cacgtagtaa
     2041 tagcccgtgc cggctgagct cacgagcttg atcttttggg tgacgggctt ggccatctcg
     2101 gaaaactctc tctagcgaat tggcgcgaac cctacccgat caagacttga agtcaagagc
     2161 gggggaggcg cgctgggacg acgacctgct catcttcggg gacgacgaag cgctcgatac
     2221 cgtcctcggt cgcattcgga tcgaagccat gcatgcgaag tgcccattgc agcccgatca
     2281 atccgccctt gaccgacggc aataggatga gggcaaggat gatggtcagc ggcccccaca
     2341 gcgcagcatg cacccagaga ggcggccggt acgtcatctc gaccaaaagc acgagagcga
     2401 ccacgatgtg gccgacgata acgacagtaa aataaggcgg cgcatcgtcg gcgcggtgat
     2461 gatgcaactc ttcgccgcaa tgcgggcagg tgtcagagac cttcaggaat cgccaataaa
     2521 gtgcgccaac cccgcaagcc ggacatttgt gtttggcgcc gcgcagc
//
LOCUS       DATDMW010000094         5729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_896968, whole genome shotgun sequence.
ACCESSION   DATDMW010000094 DATDMW010000000
VERSION     DATDMW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896968"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VI319_03940"
     CDS             complement(<1..332)
                     /locus_tag="VI319_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6863027.1"
                     /translation="MRLTQIKLAGFKSFVDPTSISIPGQLVGVVGPNGCGKSNIMDAV
                     RWVLGESRAAALRGDSMQDVIFNGTKTRKPASRSSVELVFDNADHRAGGQWAQFAEIA
                     VKRVLTRDG"
     gene            415..831
                     /gene="queF"
                     /locus_tag="VI319_03945"
     CDS             415..831
                     /gene="queF"
                     /locus_tag="VI319_03945"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY6863028.1"
                     /translation="MPSKPSKTLDTFPNPNPGRDYRIHMRIPEFTCLCPKTGQPDFAT
                     LFLDYVPDRTCVELKSLKLYIWSFRDEGAFHEVVTNRIVDDLVAAIAPRYLRLTARFF
                     VRGGIYTTVVAEHRKPGWKAPEPVALDGLPDRESAG"
     gene            828..2018
                     /gene="dapC"
                     /locus_tag="VI319_03950"
     CDS             828..2018
                     /gene="dapC"
                     /locus_tag="VI319_03950"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY6863029.1"
                     /translation="MNPRLALLQPYPFEKLRRLFEGVTPPAGVAHISLGIGEPRHPTP
                     ELVKRALTASLAKLAAYPPTAGGEDLRRAIADWLMRRYALPAVDWKTEVLPVNGSREA
                     LFAFAQAVIDPGRAGAAVLCPNPFYQIYEGAALLAGAQPVYVNTLAGRGFRSDYGSLP
                     EETLRRAQLLYVCSPGNPTGHVAGLEEWREIFALADRHGIVIASDECYSEIYFDEAAP
                     PLGALQAAHRLGRGFERLVVFSSLSKRSNVPGMRSGFVAGDAAILKSFLLYRTYHGGA
                     MSAAVQDASAAAWRDEAHVRDNRARYREKFDRVMPIVGEALDVGRPDAAFYLWARTPI
                     SDTEFARRLYAEQHVTVLPGSYLARTSGGVNPGEGRIRMALVADMDECVEAVRRVVEF
                     TRTL"
     gene            2015..2836
                     /gene="dapD"
                     /locus_tag="VI319_03955"
     CDS             2015..2836
                     /gene="dapD"
                     /locus_tag="VI319_03955"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY6863030.1"
                     /translation="MSELQNIIEQAWEDRAKLSPGSAPAKVGEAVGQVLEALDQGRLR
                     VAEKTGGEWRTHQWLKKAVLLSFRLEENRPVHAGALQFYDKVPTKFERYDAERFARGG
                     FRVVPPAVARRGAYIAKNVVMMPSYVNVGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVVVEENSVLSMGVFIGQSTRIYDRA
                     TGRILQGRVPAGSVVVPGTLPSPDGKYGLYCAVIVKRVDAQTRAKTSINDLLRGE"
     gene            2873..4048
                     /locus_tag="VI319_03960"
     CDS             2873..4048
                     /locus_tag="VI319_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY6863031.1"
                     /translation="MSAMKRLFQLMAEKKASDIFLSVKSPINIKINGVAVPVNQQLLE
                     ANSILALLYEVLNERQRKEFEQTLELNIGYTLEGVGTFRISAFRQKGSPAVVVRYIPG
                     TIPPIDSLGLPPALKEIIMEKRGLLLMVGATGSGKSTSLAAMLDYRNEQKSGHILTLE
                     DPIEFVFQNKKSIVNQREVGTDTLDFKVGLKNALRQAPDCILIGEIRDKDTMSSAIAY
                     AQSGHLCLATLHANNSYHAMNRIISFYPLENRPALLEDLASTLKCVVSQRLVRNKQGL
                     RSAAVEIMLNTRHLQELIAKGEIEQMKEAMEKSLAPGSQTFEQALFKMIKDGVITQEE
                     GLANAASATNLLWLINNTQAGAPPADGGGERRYVPTETSPGSSFSDFKLDVGETASH"
     gene            4048..5184
                     /gene="dapE"
                     /locus_tag="VI319_03965"
     CDS             4048..5184
                     /gene="dapE"
                     /locus_tag="VI319_03965"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY6863032.1"
                     /translation="MQPDAATLALARELISRRSVTPEDGGCQPLLAQRLRALGFEVQA
                     LRSGDVDNLWARRGNAAPLVCFAGHTDVVPTGPLGQWHTDPFVPTERDGKLFGRGAAD
                     MKTSIAAFVVALERFLAERPKHRGSIALLITSDEEGLAVDGTVRVVERLKARGERIDY
                     CVVGEPTCVSRLGDTVKNGRRGSLSGRIKVKGLQAHIAYPHLGKNPIHLVAPAFAELS
                     TTEWDKGNEYFPPTTWQISNVHAGTGAENVIPGELEAAFNFRFSTASSPEDLKRRVHE
                     VLDRHRLDYELQWILGGRPFLTPRGELVDTVSAAIEEVTGLRPEISTTGGTSDGRFIA
                     DVCPQVVEFGPVNASIHKLNEHIELAAIGPLQEIYRRVLLKLLG"
     gene            5187..>5729
                     /locus_tag="VI319_03970"
     CDS             5187..>5729
                     /locus_tag="VI319_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HEY6863033.1"
                     /translation="MAAAPVTVGESLARAEEAFSRAHLHFGHGTHNAADEAAWLVTHA
                     LRIPHERLGASLSRPLTPAERAKVSHLVSERVRRRVPLAYLLREAWLGEHRFYVDERV
                     IVPRSYIAELLREGLAPWAGDPSRVTRALDMCTGSGCLAVLLALAFPRARVHAVDVSP
                     GALAVARRNVAAYRLKSRVRL"
BASE COUNT          914 a         2009 c         1933 g          873 t
ORIGIN      
        1 ccgtcgcgcg tcaacacgcg cttgacggcg atctcggcga actgcgccca ctgcccgccg
       61 gcccggtggt cggcgttgtc gaacaccaat tccaccgacg aacgcgaagc gggcttgcgg
      121 gtcttggtgc cgttgaagat cacgtcctgc atcgagtcgc cgcgcagcgc cgccgcccgg
      181 gactccccga gcacccagcg caccgcgtcc atgatgttcg acttgccgca gccgttcggg
      241 ccgacgacgc cgacgagctg gccggggatg ctgatggagg tcggatcgac gaacgacttg
      301 aagccagcga gcttgatctg agtgaggcgc acttgctccc ttttcttgcg cctgcgttgt
      361 ccaatagcgc ggcgcttata ataccaattt tcctccgtcg attccgtttt gtggatgccc
      421 tcgaagccct cgaaaacgct tgacacgttc ccgaatccga accccgggcg cgactaccgc
      481 atccacatgc gaatccccga gttcacctgt ctctgtccga agacagggca gccggatttc
      541 gccacgcttt tcctcgacta cgtccccgac cggacgtgcg tcgagctgaa gagcctcaag
      601 ctctacatct ggtcgttccg cgacgaaggc gcgttccacg aggtggtcac gaaccgcatc
      661 gtcgacgacc tcgtggccgc gatcgcgccg cgctacctgc gcctgacggc gcgcttcttc
      721 gtgcgcggcg gaatctatac gaccgtggtc gccgagcacc gcaagccggg ctggaaggcg
      781 cccgagcccg tggcgctcga tgggctgccc gatcgagaga gcgcgggatg aatccgcgcc
      841 tcgcgctgct ccagccctac ccgttcgaga agctgcgcag actgttcgag ggcgtgacgc
      901 cgccggccgg cgtcgcgcac atcagcctcg gcatcggcga gccgcgccat ccgacgccgg
      961 agctcgtgaa gcgcgcgctg acggcgagcc tcgccaagct cgccgcgtac ccgcccaccg
     1021 ccggcggcga ggacctgcgc cgcgcgatcg ccgactggct gatgcgccgc tacgcgctgc
     1081 cggcggtcga ctggaagacc gaggtcctgc ccgtcaacgg ctcgcgcgag gcgctgttcg
     1141 cgttcgcgca ggcggtgatc gaccccggtc gcgcgggcgc cgcggtcctg tgcccgaatc
     1201 cgttctacca gatctacgag ggcgcggcgc tgctcgcggg ggcgcagccc gtgtacgtca
     1261 acacgctcgc cgggcgcggc ttccgctccg attacggatc gctcccggag gagacgctgc
     1321 ggcgcgcgca gctcctgtac gtgtgctcgc ccggcaaccc gaccggccat gtcgccggcc
     1381 tcgaggagtg gcgcgagatc ttcgcgcttg ccgaccgcca cggcatcgtg atcgcatccg
     1441 acgagtgcta ctccgagatc tatttcgacg aggcggcgcc gccgctcggc gcgctccagg
     1501 ccgcgcaccg cctcggccgc ggcttcgagc gcctggtcgt gttctcgagc ctgtcgaagc
     1561 gctcgaacgt tccggggatg cgctcgggct tcgtcgccgg ggatgcggcg atcctgaagt
     1621 cgttcctgct ctatcgcacc tatcacggcg gcgcgatgag cgccgcggtg caggacgcga
     1681 gcgccgcggc ctggcgcgac gaggcgcacg tgcgcgacaa ccgggcgcgc taccgcgaga
     1741 aattcgaccg cgtcatgccg atcgtcggcg aggctctcga tgtcgggcgg cccgatgcgg
     1801 cgttctacct ctgggcccgc acgccgatct ccgacaccga attcgcgcgg cggctgtacg
     1861 ccgagcagca cgtcaccgtt cttcccggga gctacctcgc ccggacttcg ggcggcgtca
     1921 accccgggga aggccggatc cgcatggccc tggtcgccga catggacgag tgcgtcgagg
     1981 cggtccggcg cgtcgtagaa ttcacgagga cgctatgagc gagctgcaga acatcatcga
     2041 gcaggcctgg gaggaccgcg cgaagctctc gccgggatcg gctcccgcga aggtcggcga
     2101 ggcggtcggc caggtcctcg aagccctgga ccagggacgg ctgcgcgtcg ccgagaagac
     2161 gggcggcgaa tggcgcaccc accagtggct gaagaaggcg gtgctgctgt cgttccggct
     2221 cgaggagaac cgtcccgtgc acgccggcgc gctgcagttc tacgacaagg tgccgaccaa
     2281 gttcgagcgc tacgacgccg agcgcttcgc gcgcggcggc ttccgggtcg tgccgccggc
     2341 ggtggcgcgc cgcggcgcgt acatcgcgaa gaacgtcgtc atgatgccct cgtacgtgaa
     2401 cgtcggcgcc tacgtcgacg agggcacgat ggtcgacacc tgggcgaccg tgggctcgtg
     2461 cgcgcagatc ggcaagaacg tgcacctctc gggcggcgtc gggatcgggg gcgtgctgga
     2521 gccgcttcag gcgaacccca cgatcatcga ggacaactgc ttcatcggcg cgcgctccga
     2581 ggtcgtcgag ggcgtggtgg tcgaggagaa ctcggtgctg tcgatgggcg ttttcatcgg
     2641 gcagagcacg cgcatctacg atcgcgcgac cggccgcatc ctgcagggcc gcgttcccgc
     2701 gggatccgtc gtcgttccgg gaactcttcc ctcccccgac ggcaaatacg gcctatactg
     2761 tgccgtgatc gtgaagcggg tcgacgcgca gacgcgcgcg aagacgagca tcaacgacct
     2821 gctgcgcggc gaataacaaa gatccggcgc tcgcgaatcc agggagagca gcatgtcggc
     2881 gatgaaacgg ctgttccagc tgatggccga gaagaaggcc tcggacatct tcttgtcggt
     2941 caagtcgccg atcaacatca agatcaacgg cgtcgcggtg ccggtgaacc agcagctgct
     3001 cgaggcgaac tcgatcctgg cgctgctgta cgaggtgctg aacgagcgcc agcgcaagga
     3061 gttcgagcag accctggagc tcaacatcgg ctacaccctc gaaggcgtgg gcacgttccg
     3121 catcagcgcg ttccgccaga agggctcgcc tgcggtcgtc gtgcgctaca ttcccgggac
     3181 catcccgccg atcgactcgc tcgggctgcc ccccgcgctg aaggagatca tcatggagaa
     3241 gcgcgggctc ctgctcatgg tcggcgcgac cggctccggg aagtcgacgt cgctcgcggc
     3301 catgctcgac taccgcaacg agcagaaatc cggccacatc ctgacgctcg aggacccgat
     3361 cgagttcgtg ttccagaaca agaagtcgat cgtgaaccag cgcgaggtcg ggaccgacac
     3421 gctcgacttc aaggtcgggc tgaagaacgc gctgcggcag gcgcccgact gcatcctgat
     3481 cggcgagatc cgcgacaagg acaccatgtc gtcggcgatc gcgtacgccc agtcggggca
     3541 tttgtgcctc gcgacgctgc acgcgaacaa cagctatcac gcgatgaacc ggatcatcag
     3601 cttctatccg ctcgagaacc ggcccgcgct gctcgaggat ctcgcgtcga cgctcaagtg
     3661 cgtggtgtcg cagcgcctgg tgcgcaacaa gcaggggctg cgcagcgccg cggtcgagat
     3721 catgctcaac acgcggcacc tgcaggagct gatcgcgaag ggcgagatcg agcagatgaa
     3781 ggaggccatg gagaagagcc tggctcccgg ctcgcagacc ttcgagcagg cgctgttcaa
     3841 gatgatcaag gacggcgtca tcacgcagga agaaggcctc gccaacgcgg cctccgcgac
     3901 caacctgctc tggctcatca acaacaccca ggcgggcgcg ccgcccgccg acggcggcgg
     3961 cgagcgccga tacgttccca ccgagacctc gccgggcagc tcgttcagcg acttcaagct
     4021 cgacgtcggg gagaccgcat cgcattgatg cagcccgacg cggccaccct cgcgctcgcg
     4081 cgcgagctga tctcccggcg atccgtaact cccgaggacg gcggctgcca gcccctgctg
     4141 gcgcagcgcc tgcgcgcgct cggcttcgag gtgcaggcgc tgcgcagcgg cgacgtcgac
     4201 aacctctggg cgcgccgcgg caatgccgcg ccgctcgtct gcttcgccgg ccataccgac
     4261 gtcgtgccga cgggtccgct cggccagtgg cacaccgatc ctttcgtgcc gaccgagcgc
     4321 gacggcaagc tgttcgggcg cggcgccgcc gacatgaaga cctcgatcgc ggcgttcgtg
     4381 gtcgcgctcg agcgcttcct cgccgagcgc ccgaagcacc gcggctcgat cgcgctcctc
     4441 atcacgtccg acgaggaagg cctcgcggtc gacggcacgg tgcgcgtggt cgagcgcctc
     4501 aaggcgcgcg gcgagcgcat cgactactgc gtggtcggcg agccgacctg cgtgagccgc
     4561 ctgggcgaca cggtcaagaa cggccgccgc ggatcgctct cgggccggat caaggtcaag
     4621 ggactgcagg cgcacatcgc ctatccccat ctcgggaaga acccgatcca tctcgtcgcg
     4681 cccgccttcg ccgagctctc gaccaccgag tgggacaaag gcaacgagta tttcccgccg
     4741 accacgtggc agatctcgaa cgtgcacgcg gggacgggcg cggagaacgt gatcccgggc
     4801 gagctcgagg ccgcgttcaa cttccgcttc tcgaccgcga gctcgcccga agatctcaag
     4861 cgccgcgtgc acgaggtgct cgaccgccac aggctcgact acgagctcca gtggatcctc
     4921 ggcggcaggc cgttcctcac gccgcgcggc gagctggtgg atacggtgag cgccgcgatc
     4981 gaagaggtca ccgggctgcg gccggagatc agcaccaccg gcgggacgtc cgacggccgc
     5041 ttcatcgccg acgtctgccc gcaggtcgtc gagttcggcc cggtcaacgc gtcgatccac
     5101 aagctgaacg agcacatcga gctggcggcg atcggcccgc tgcaggaaat ctaccggcgg
     5161 gtgctgctga agctgctcgg ctgaggatgg ccgcggcgcc cgtcaccgtg ggcgaatccc
     5221 tcgcgcgcgc ggaggaagcc ttctcccgcg cccatctcca tttcgggcac ggaacccaca
     5281 acgccgcgga cgaggccgca tggctcgtga cgcacgcgct gaggattccg cacgagcggc
     5341 tcggcgcgtc gctctcgcga cccctgacgc cggccgagcg cgcgaaagtg tcgcacctcg
     5401 tgtcggagcg cgtgcgccgc cgcgtcccgc tcgcctacct tctgcgcgag gcctggctcg
     5461 gggagcaccg tttctacgtc gacgagcgcg tgatcgtgcc gcgctcgtac atcgccgagc
     5521 tcctgcgcga aggcctcgcg ccctgggccg gggatccttc gagggtgacc cgggcgctcg
     5581 acatgtgcac cggctcgggg tgcctcgcgg tgctgctcgc gctcgccttc ccgcgcgcgc
     5641 gcgtccacgc ggtcgacgtc tcgcccggcg cgctggccgt cgcccggcgc aacgtcgcgg
     5701 cgtatcgcct gaagtcgcgc gtgcggctg
//
LOCUS       DATDMW010000095         3220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_275077, whole genome shotgun sequence.
ACCESSION   DATDMW010000095 DATDMW010000000
VERSION     DATDMW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275077"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VI319_03975"
     CDS             <1..449
                     /locus_tag="VI319_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6863034.1"
                     /translation="GIKLVHVPYKGAGQSIPDVIGGQVPIGVYGILTISPQAKAGKLR
                     ILAVTTPKRSPIAPDVPTMAESGFPGFDTSLWFGLLAPAGTPKDVIAKVHDDAVRALG
                     LPDVAERIAAQGGEVIGSTPAQFAAFIAAEDAKYAKIIREAGVRLE"
     gene            complement(452..841)
                     /locus_tag="VI319_03980"
     CDS             complement(452..841)
                     /locus_tag="VI319_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863035.1"
                     /translation="MRVFRIFTGPSGESAIEVRRVPMSGGERPMSRSFGCESIFFRET
                     PEGHVQDLHNAPRRQLIFLVSGLLELESSTGRRFVCRPGDLIFAEDTTGRGHITRSLR
                     DVRGFVHVAMPADFDVSGWPLEGPENP"
     gene            1001..1984
                     /locus_tag="VI319_03985"
     CDS             1001..1984
                     /locus_tag="VI319_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY6863036.1"
                     /translation="MKKILVTGGAGFLGSHLCDALIDAGHEVLCVDNLFTGSKQNVAH
                     LLGEPRFEFMRHDVTFPLYVEVDEIYNLACPASPVHYQFDPVQTTKTSVHGAINMLGL
                     AKRTRARVFQASTSEVYGDPEMHPQQEQYWGRVNPIGIRACYDEGKRCAETLFFDYHR
                     QHGLEIKVARIFNTYGPRMHPDDGRVVSNFIVQALRGDDITIFGDGSQTRAFCYVEDM
                     IRGFLSLMATEHCVTGPINLGNPAEYTVAELAHKVVEMTGSASRVVYRPLPSDDPRQR
                     RPDIRLARERLGWAPQVALEDGLMETILYFDRLLGQREEQVPVRAAARAAA"
     gene            1996..2970
                     /gene="galE"
                     /locus_tag="VI319_03990"
     CDS             1996..2970
                     /gene="galE"
                     /locus_tag="VI319_03990"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY6863037.1"
                     /translation="MPRRAVLVTGGAGSIGSHACKALARAGHLPVTLDSLVHGHASAV
                     RWGPLVQGDVRDRALLERTMREHRVAAVVHFAAYAYVGESMRDPGKYFGNNVAGTLSV
                     LDAMRACDVRPIVFSSSCATYGVPRSLPIGEDHPQDPINPYGESKRVAERALHWYGAA
                     HGLRSAALRYFNAAGADLDREIGEDHDPETHLVPLAIGAALGTQPPLAVCGTDYPTAD
                     GTAVRDYVHVSDLADAHALALDYLLDGGASVALNLGTGRGHSVREVVAAVERVARRDV
                     PRRDVARRPGDPACLVADAARARELLGWEPRCSGLETIVDTAWRWHAR"
     gene            2949..>3220
                     /locus_tag="VI319_03995"
     CDS             2949..>3220
                     /locus_tag="VI319_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863038.1"
                     /translation="MALARALSAEPALDAPVPRAPKTLDARPRVAGKFLYAGDEKLWL
                     KGVTYGTFRPGPGGDYPQPEQLDADFSAMAAAGINAVRVYTVPPRAL"
BASE COUNT          468 a         1196 c         1110 g          446 t
ORIGIN      
        1 cgggcatcaa gctcgtgcac gtcccgtaca agggcgcggg ccagtcgatc cccgacgtga
       61 tcgggggcca ggtgccgatc ggcgtctacg gcatcctcac catcagcccg caggcgaaag
      121 cgggcaagct gcgcatcctg gccgtgacca cgccgaagcg ctcgccgatc gccccggacg
      181 tgccgacgat ggccgagtcg ggcttcccgg gcttcgacac gtcgctgtgg ttcggcctgc
      241 tcgcgccggc ggggacgccg aaggacgtga tcgcgaaggt ccacgacgac gcggtgcgcg
      301 cgctcgggct gcccgacgtc gccgagcgca tcgccgccca gggcggcgag gtgatcggca
      361 gcacgccggc gcagttcgcg gcgttcatcg cggccgagga cgcgaagtac gcgaagatca
      421 tccgcgaggc gggcgtgcgg ctcgagtagc cctacgggtt ctccggcccc tccagcggcc
      481 agccggagac gtcgaagtcc gccggcatcg ccacgtgcac gaacccgcgg acgtcgcgca
      541 gcgagcgcgt aatgtggccg cggccggtcg tgtcctcggc gaagatgagg tccccgggcc
      601 ggcacacgaa gcgccggccc gtgctcgact ccagctcgag cagcccggac acgaggaaga
      661 tgagctgccg ccgaggcgcg ttgtgcaggt cctggacgtg gccctcgggc gtctctcgga
      721 aaaagatcga ctcgcagccg aagctccgcg acatggggcg ctcgccgccg ctcatcggca
      781 cgcggcggac ctcgatcgcc gactcgcccg acggcccggt gaagatgcgg aacacgcgca
      841 tcgcgcgatc ctagccggtt tcgggcgagc gcacccgtca acgtctccgc gcgcggacac
      901 tccgtaagga ccatggagcg gctgctctcc gtcatgaggg gagccggggt tcgctcttta
      961 acatcggcat cggcacatca catcgggttg actcctctgc atgaaaaaga tactcgtcac
     1021 cggcggcgcc ggtttcctgg gctcgcacct gtgcgacgcc ctgatcgacg cggggcacga
     1081 ggtcttgtgc gtcgacaatc tgttcaccgg cagcaagcag aacgtcgctc acctgctggg
     1141 cgagccgcgc ttcgagttca tgcgccacga cgtcaccttc ccgctgtacg tggaggtcga
     1201 cgagatctac aacctcgcct gcccggcctc gcccgtgcac taccagttcg acccggtgca
     1261 gacgacgaag accagcgtgc acggcgcgat caacatgctg ggcctcgcga agcgcacgcg
     1321 cgctcgcgtg ttccaggcgt cgacctcgga ggtctacggc gatcccgaga tgcacccgca
     1381 gcaggagcag tactgggggc gcgtcaaccc gatcggcatc cgcgcctgct acgacgaggg
     1441 caagcgctgc gccgagacgc tgttcttcga ctaccaccgg cagcacggcc tcgagatcaa
     1501 ggtcgcgcgc atcttcaaca cctacggccc gcgcatgcat cccgacgacg gccgcgtggt
     1561 gtcgaacttc atcgtgcagg cgctgcgcgg cgatgacatc acgatcttcg gcgacggcag
     1621 ccagacgcgc gccttctgct acgtcgagga catgatccgc gggttcctct cgctcatggc
     1681 gaccgagcac tgcgtcaccg gcccgatcaa cctcggcaac ccggccgagt acacggtggc
     1741 cgagctcgcg cacaaggtcg tcgagatgac cggttcggcg tcgcgcgtcg tgtatcgccc
     1801 gctgccgtcc gacgatccgc gccagcggcg ccccgacatc cggctcgcgc gcgagcgcct
     1861 gggctgggcg ccgcaggtcg cgctcgagga cgggctcatg gagaccatcc tctacttcga
     1921 ccgcctcctc ggccagcgcg aggagcaggt cccggtgcgc gccgcggcgc gcgccgccgc
     1981 ctgaggcacg agcgcgtgcc ccggcgcgcc gttctcgtga cgggaggggc gggctccatc
     2041 gggagccacg cctgcaaggc gctcgcgcgc gcgggccacc tgccggtcac gctcgacagt
     2101 ctcgtgcacg gccacgcctc ggccgtgcgc tggggaccgc tcgtccaggg cgacgtgcgc
     2161 gaccgcgcgc tcctcgagcg cacgatgcgc gagcaccgcg tcgcggcggt cgtgcacttc
     2221 gccgcctacg cctacgtggg cgagtcgatg cgcgatcccg gcaagtactt cggcaacaac
     2281 gtcgccggga cgctctcggt gctcgacgcc atgcgcgcct gcgacgtgcg cccgatcgtg
     2341 ttctcctcga gctgcgcgac gtacggcgtg ccgcgctcgc tgccgatcgg cgaggatcat
     2401 ccgcaggatc cgatcaaccc gtacggcgag tcgaagcgcg tcgccgagcg cgcgctgcac
     2461 tggtacggcg cggcccacgg cctgcgctcg gccgcgctgc gctacttcaa cgccgccggc
     2521 gccgatctcg accgtgagat cggcgaggac cacgatcccg agacgcacct cgtcccgctc
     2581 gcgatcgggg ccgcgctcgg cacgcagccg ccactcgcgg tctgcggcac cgactacccg
     2641 accgccgacg gcacggcggt gcgcgactac gtgcacgtga gcgatctcgc tgacgcgcac
     2701 gcgctcgcgc tcgactacct cctcgacggc ggcgccagcg tcgcgctcaa cctgggcacc
     2761 gggcgcggcc actcggtgcg cgaggtcgtc gcggcggtcg agcgcgttgc gcgccgcgac
     2821 gtgccgcggc gcgacgtcgc ccgccgtccc ggcgatcccg cgtgcctggt cgccgacgcg
     2881 gcccgggcgc gcgagctgct gggctgggag ccgcgatgct ccgggctcga gacgatcgtc
     2941 gacacggcgt ggcgctggca cgcgcgctga gcgccgagcc ggcgctggat gcgccggtgc
     3001 cccgcgcgcc gaagacgctc gacgcgcgtc cgcgcgtcgc cgggaaattc ctctacgcgg
     3061 gagacgagaa gctctggctc aagggcgtca cgtacggcac tttccgcccc ggacccggcg
     3121 gcgactatcc gcagcccgag cagctcgacg ccgacttctc cgccatggcg gccgccggca
     3181 tcaacgcggt gcgcgtctac accgttccgc cgcgcgccct
//
LOCUS       DATDMW010000096        19475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1271652, whole genome shotgun sequence.
ACCESSION   DATDMW010000096 DATDMW010000000
VERSION     DATDMW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271652"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="VI319_04000"
     CDS             <1..170
                     /locus_tag="VI319_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY6863039.1"
                     /translation="QVGYFKWSGGKRIFRMAPVHHHFELKGWHENQVVVRAWIITMLL
                     VLAGLSSLKLR"
     gene            194..1555
                     /gene="murD"
                     /locus_tag="VI319_04005"
     CDS             194..1555
                     /gene="murD"
                     /locus_tag="VI319_04005"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY6863040.1"
                     /translation="MLGLGDTGLSMAHWLTRRQAKVRVADSREAPPGLDALGRSLPGL
                     APVLGPFREESFAGVDLIAASPGVPLAEPRLQQALRAGVEVVGDIELFARSLEAARRP
                     LVLGVTGTNGKSTVTALAGAMARAAGSDCEVAGNIGPPVLDALMRREDEARAAKLWVL
                     ELSSFQLETTSSLDASAAVVLNITEDHLDRYPGMAEYAAAKARIFRGDGVQVLNRNDP
                     RTLAMALPGRTVLTFGLDAPPGPRDYGILARSVEDWLAEGAEALMPVKAMKLPGRHNA
                     ANALAALALCRAVGLPMAPLLGALREFRGLPHRVEPVGEIGGVRYYDDSKGTNVGASV
                     AAIAGLAHDHPGIVLIAGGEGKGQDFAPLREPLARSARAVVLIGRDGPLIEKALSGAG
                     VPLRKAAGMQEAVTLAAAEARPGDAVLLSPACASFDMFRNYAHRGEVFRATVEGMARG
                     DAR"
     gene            1539..2699
                     /gene="ftsW"
                     /locus_tag="VI319_04010"
     CDS             1539..2699
                     /gene="ftsW"
                     /locus_tag="VI319_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006580277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY6863041.1"
                     /translation="MATLVSAGRPQAPEYDSGLLWAAVLLLGFGLVMVYSASIAMAEA
                     SAFTGGRATYFLMRHAVFLVFAVIAGMLAFQVPMRLWQRAAPALFLASAAMLALVLVP
                     GIGREVNGARRWIGLGPAGLQPSEFMKLFAVLYAADYTVRKSALMQSFKQGLLPMLAV
                     MLVVGGLLLQEPDFGAFVVIAAIATGILFLGGMNGKWFAGLLVMLALGFAGLVLSSPY
                     RMQRIFGFMDPWSDPFGRGYQLSHALIAFGRGEWFGVGLGGSVEKLLYLPEAHTDFLL
                     AVIAEELGFAGVLAVIVLFSWLVLRAFAIGRQAVALERPYWALAAQGIGIWIGAQTLI
                     NMGVNMGVLPTKGLTLPLMSFGGSGLVANCIALAVLLRIDWESRQMMRGRPG"
     gene            2710..3771
                     /gene="murG"
                     /locus_tag="VI319_04015"
     CDS             2710..3771
                     /gene="murG"
                     /locus_tag="VI319_04015"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY6863042.1"
                     /translation="MSRTLMIMAGGTGGHIFPALAVADRMREAGWRVVWLGARTGMEA
                     RLVPARGYEMAWVAFSGVRGKGPLALALLPTRLLVAFWQSARAIFSRRPDVVLGMGGY
                     VSFPGGMMAALLGRPLVIHEQNSIAGLANKVLARVADRVLASFPGALPGAVVTGNPVR
                     PEIGALEGPEIRYAGRSGPLRLLVVGGSLGAKALNDAVPAAMALVPRERRPVIRHQAG
                     AKHIEALRAAYAAAGVEAETVPFIDDMAKAYAEADLVVCRAGATSIAEIAAAGVASVL
                     VPFPFAVDDHQTTNARLLADANAGVLLPQSQLTPERLSALVLSFDRGRLLDMARRARA
                     LGRPDAARAVAQACEALAR"
     gene            3768..5177
                     /gene="murC"
                     /locus_tag="VI319_04020"
     CDS             3768..5177
                     /gene="murC"
                     /locus_tag="VI319_04020"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY6863043.1"
                     /translation="MKYKVKRVHFVGIGGAGMSGIAEVLLKLGYQVSGSDVAESATTQ
                     RLEERGAKVRIGHDPDHVSGADVVVISTAIKHDNPEIAAARAGHIPVVPRAMMLAELM
                     RLKQGIAVAGTHGKTTTTSLIASVLAEAGEDPTFVIGGRLNSAGSHAKLGSGDFLVVE
                     ADESDASFLHLQPVVAVVTNIDVDHMETYNHDFSGLKRTFIDFLQRLPFYGAAVVCLD
                     DPGVSEIIPLVSRRLITYGLRSDADIRALGVEHVAGKMRFRAERAPRLLRRGQKAALE
                     IQLNAAGAHNVLNALAAIGVAQEIGLDDGAVVRALANFQGVGRRLQRYGDVTLRRGCT
                     VTVVDDYGHHPAEMRVTLEALRGAFPGRRLLLAFQPHRYTRTRDLFGSFVEVLSGVDE
                     LLLADVYPAGEAAIAGADGKALYQAVRRFDAVKASFVGNISDMAEAIRKAAQNGDVVV
                     TMGAGSIGTVPAALLKTAR"
     gene            5174..6100
                     /gene="murB"
                     /locus_tag="VI319_04025"
     CDS             5174..6100
                     /gene="murB"
                     /locus_tag="VI319_04025"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY6863044.1"
                     /translation="MKMTEPIHKPAAALRGTLRLNEPMARHVSWRAGGAAARAYLPAD
                     TADLALFLRTLPAGEPVTFVGLGSNLLVRDGGYRGTVVLLHNTRAAVRLDGPLVYAEA
                     GVAAPKVARFAGTHALEGAEFLAGIPGTVGGALAMNAGCYGSETWDIVARVQTVSRSG
                     DLREHGREQFDIGYRHVALRGARLGEDEWFVAGWFALRAGDGARARARVKQLLGRRIA
                     TQPLGLPNAGSVFRNPPGDHAARLIEVCGLKGYAAGGARISEKHANFIVNPEGGARAA
                     DIERLIEHARAEVKRRHGVDLVPEVRIIGEAT"
     gene            6097..7041
                     /locus_tag="VI319_04030"
     CDS             6097..7041
                     /locus_tag="VI319_04030"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY6863045.1"
                     /translation="MKPADFGKVAVLLGGKSAEREVSLNSGRMVLAALRAKGVDAHPF
                     DPRDRGLDQLIAARFDRVFIALHGRYGEDGTLQGALELLGLPYTGSGVLASALAMDKW
                     RTKLVWQSCGVPTPHSEVLTPQTDFEAVANRVGLPLMVKPANEGSSIGMSKVRTAAAL
                     ADAYALAAKYDSLVIAESFVEGVELTGAVLGDTPLPLIRLETPHDFYDYDAKYKAADT
                     RYIIPCGLPPDAERVIQDEVLRAFRAVGCSGWGRVDLILDRSGKPYFLEVNTSPGMTD
                     HSLVPMAARHAGIAFEDLCLRILELARVTTKTGGDGVG"
     gene            7031..7780
                     /locus_tag="VI319_04035"
     CDS             7031..7780
                     /locus_tag="VI319_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY6863046.1"
                     /translation="MWDSPRLLNLAAGVLLAAAVALVLAGAAAYAMRSPAFVLNSIRV
                     VGDVDHVAVPQVIDALQGRVSGTFFSVDIDGIRALFETLPWVRRAEVRRLWPDGLEVR
                     IEEHVPLARWGQERDGRLVNSYGEPFRGRVEGALPAFFGPPGSEGMLARRYADFRRVL
                     APLSIEPASVLLSARYAWQLQLSSGLIVKLGRDRDKDDAVQRLARFVAVYPYTLGKAQ
                     RRFEYVDLRYPNGFAVRAADARSLHERLPRT"
     gene            7780..9009
                     /gene="ftsA"
                     /locus_tag="VI319_04040"
     CDS             7780..9009
                     /gene="ftsA"
                     /locus_tag="VI319_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY6863047.1"
                     /translation="MPKDNKNLVVGLDIGTSKVVAMVAELMPDGRMDVIGMGSHESKG
                     LKKGVVVNIESTVSAIQRALEEAELMADCKIQRVFTGIAGSHIKSFNSTGMVAIKEKE
                     VTPLDVDRAIETARAMPIPTDQQVLHILTQEFIIDGQDGVREPLGMSGVRLEVKVHIV
                     TGAVSAAQNIVKCVRRCGLEVSDLILQPLASSLAVLTEDEKELGVCLVDIGGGTTDIA
                     VFREGAIRHTAVIPIAGDQITNDVAMALRTPTAEAEELKVRHGCALRQLAEAGQMIEV
                     PGVGDRPPRMLSRQTLAEVIEPRVEELYSLIQQVLRDSGYEELLSSGLVLTGGSAVMQ
                     GMVELGEEIFHMPVRLGVPRYSGGLSDVVRSPRYATAVGLLMEGVTQTRRGQVARQGG
                     SFKQVMGRMREWVQRNF"
     gene            9061..10212
                     /gene="ftsZ"
                     /locus_tag="VI319_04045"
     CDS             9061..10212
                     /gene="ftsZ"
                     /locus_tag="VI319_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY6863048.1"
                     /translation="MFEIVENSSGGTVIKVIGVGGAGGNAVDHMIRNGVCGVEFIAAN
                     TDAQALSRGLAKQQLQLGATGLGAGAKPEAGRAAALEARDQLAESLRGAHMVFITAGM
                     GGGTGTGAAPVVAEVAKEMGILTVAVVTKPFSFEGSRRMQSAEAGIEELSQHVDSVIV
                     ILNEKLEEVLGDDISMEEAFGTADSVLKNAVAGISEIINVPGLINVDFQDVRTVMAEQ
                     GMAMMGSAASSGVDRARIAAEQAVACPLLEGVNLSGARGVVVNITADKSSLKLRETKE
                     VMNTIRGFAAEDATIIFGAVYDDAMGDELRVTVVATGLGQPQAARQPKPQLVQRTGTD
                     NLPVAGVDYRALDEMPAVIRKNRSSTIEALQNSGVDKYDIPAFLRKQAD"
     gene            10236..10385
                     /locus_tag="VI319_04050"
     CDS             10236..10385
                     /locus_tag="VI319_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863049.1"
                     /translation="MSIAKRPALPPYRRKPALARPRLRVSVPVEPVLVRTVAFMPAYQ
                     RKRIH"
     gene            10466..11380
                     /gene="lpxC"
                     /locus_tag="VI319_04055"
     CDS             10466..11380
                     /gene="lpxC"
                     /locus_tag="VI319_04055"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY6863050.1"
                     /translation="MFRQRTLKSAIRATGVGLHTGEKVAMTLRPARPNTGIVFRRVDT
                     PKPVDLRAEAFRVGDTRLSTTLEDGEVKVATIEHLMSAFAGLGVDNAYVDLSGSEVPI
                     MDGSAAPFVFLIQSAGIEEQPAAKRFFRIKRPVEVRDGDKWARFEPYEGLKLSFSIVF
                     DHPVFEKSAQEASVDFAETSYVKEVARARTFGFMQDVEAMRSSGLAQGGSLDNAIVMD
                     EYRVLNAEGLRFGDEFVKHKLLDAIGDLYLLGYPLIGAYAAHKSGHGLNNRLLRETMA
                     RKEAWELVSFERAEDAPAALSRLFALPA"
     gene            11389..11574
                     /locus_tag="VI319_04060"
     CDS             11389..11574
                     /locus_tag="VI319_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863051.1"
                     /translation="MLALRVVAILVVLGALSALALYLFTRDRRYLRFAVRLLQVAVIF
                     ALFLFGLLALERAVALA"
     gene            complement(11575..12009)
                     /locus_tag="VI319_04065"
     CDS             complement(11575..12009)
                     /locus_tag="VI319_04065"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEY6863052.1"
                     /translation="MHPHKPSALLSESDGIPALMPQARRLLELRQILASLLPGALSRA
                     CTVANYKQGKLVLFAENNAVAAKLKLLSPTIREQFSDRGVQVTEMDIGVQAPEAAQEK
                     PEKAAKLSGSAVASLAELAAQLPDSPLKQCVAALARHDVSKP"
     gene            12038..13000
                     /locus_tag="VI319_04070"
     CDS             12038..13000
                     /locus_tag="VI319_04070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY6863053.1"
                     /translation="MHIILVSNRLAKARSVTLTMAHLVAGAACFATVILGLAAAIFWV
                     SVRHADEFNIPQLQSLVLSAQQQETQKTRDFLRENLNAMAVKLGEMQAQLTRIDALGE
                     RVSALAGFKPQEFRFSEAPGRGGAVSSIPPQNLSMVEFSQKLDQLSKRMENRTDYLGI
                     LESQLFDTKMKKKLMPTIPPVDGSWNASSFGWRIDPFTGMHAMHEGIDFLAEPGTPVF
                     ASAGGVVTFADFHPQYGYMVEIDHGNDFTTRYAHNSKLLVKVGDLVHRGRKIAESGST
                     GRSTGPHVHFEVRYKGVAQDPNRFLQASARQEHNRVLQASARAR"
     gene            13141..15930
                     /gene="secA"
                     /locus_tag="VI319_04075"
     CDS             13141..15930
                     /gene="secA"
                     /locus_tag="VI319_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY6863054.1"
                     /translation="MISRFLKAIFGSRNDRLLKQYAKSVRAINALEPAVSTLSDTELA
                     AKTPELRERVAKLLEADTQEDRKAAERAALHEVLPEAFAVVREAAKRTLNMRHFDVQL
                     IGGMVLHDGKIAEMRTGEGKTLVATLPAYLNALTRKGVHIVTVNDYLASRDAEWMGRV
                     YNFLGLSVGVILTQMPHEDKQKAYGADITYGTNNEFGFDYLRDNMAMSLADKFQRGLN
                     YAIVDEVDSILIDEARTPLIISGQAEDRTEIYHRMNEVAPLLKRQEKEGAPGDFLVDE
                     KNHTVLLSEEGHEHAESFLARAGLLPEGRSLYEPAYVNLMHHMNAALRAHHLFFRDRH
                     YVVQDGEVVIVDEFTGRLMPGRRWSDGLHQAIEAKENVSIQSENQTLASSTFQNYFRM
                     YGKLAGMTGTADTEAYEFQEIYGLEVVVIPTHKKMVRIDNLDQVFRTAKEKYKAVIDD
                     IKDCYERGQPALVGTTSVENSELLSGMLQKQGIPHQVLNAKQHAREAEIVAQAGRPRM
                     ITIATNMAGRGTDIVLGGNAEKQIDLLRQNPDLPDDDREARISKLRDEWQSLHEQVVA
                     AGGLHIVGTERHESRRIDNQLRGRSGRQGDPGSSRFYLALEDPLLRIFAGDRVNAIMQ
                     RLKMPEGEAIEHVLVTRSIESAQRKVEAHNFDIRKQLLEYDNVANEQRQNIYDLRNGV
                     LESENVSDRIRALREGVVTDLFRAYVPQESVEEQWDIAGLEKAYVSELRVEVPLAKWL
                     EQDPSLDDEALLGRAVEAANRAYDSKMQGAQADSVHHLERWVLLSTLDTHWREHLAAL
                     DHLRQGIHLRGYAQKNPKQEYKREAFELFGNLLETIKLEVTRTLMSVRPPTEEELRQV
                     EEQQRTPAVENVQYQHAGYEEALAGPAPGEEEQAGDAAPKTAPFVRQMGKIGRNDPCP
                     CGSGKKYKHCHGKLA"
     gene            15930..17156
                     /gene="argJ"
                     /locus_tag="VI319_04080"
     CDS             15930..17156
                     /gene="argJ"
                     /locus_tag="VI319_04080"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY6863055.1"
                     /translation="MAVNLKAPDPADLHPVRGVRLGVAQAGIRKANRTDLLLVALDPG
                     ASAAGVFTRNRFCAAPVLVAKEHLDAGTSVRALVVNTGVANAGTGETGIARARETCRE
                     AARLLGCDASQVLPFSTGVIMEPLPADRIAAALPRCVAGLSESRWSEAAAAIMTTDTV
                     PKAASRRVRAGDASIGFTGIAKGAGMIHPDMATMLAFIAFDARVAPALLQRMVERAAA
                     ASFNRITVDGDTSTNDAFIVMATGKAGPPIESERDPGFASVYEGLEDVAKRLAQAIVR
                     DGEGATKFITVRIEQGADAAECDRVAYAIAHSPLVKTAFFASDPNLGRILAAIGYAGI
                     ADLDVSRVDVYLDDVLVAKGGARAAGYREEDGQRVMKKAEIAVRVALGRGAAGTEIWT
                     CDLSHDYVSINADYRS"
     gene            17181..18059
                     /locus_tag="VI319_04085"
     CDS             17181..18059
                     /locus_tag="VI319_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6863056.1"
                     /translation="MSDVDRLMQQGHELLARIEALLPGRPAPVDWDASIAFRWRKQAQ
                     RGVLQPVANLHKIRLSDLQGVDRQKALIEQNTRQFVEGRPANNVLLTGARGTGKSSLV
                     KAMLNKFHKQGLRLIEVEKHDLLDLPEIVDQVAKRRERFVIFCDDLSFEASEPGYLAL
                     KVVLDGSIAAPSDNVLIYATSNRRHLMPEYMQENLETKHRGEEIHPGETVEEKISLSE
                     RFGLWISFYPFDQDGYLGIVAHWLEELGCPGDFEKARQEALQWALLRGSRSGRVAWQF
                     ARDYAGRHGRTPKKPG"
     gene            18056..18991
                     /locus_tag="VI319_04090"
     CDS             18056..18991
                     /locus_tag="VI319_04090"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HEY6863057.1"
                     /translation="MTPIEVAVAVFVRADGAVLLARRPAGKVYAGYWEFPGGKVEPGE
                     AMAAALRREIREELGADVDRAYPWITRVFVYPHATVRLHFHRVVAWRGEPRALEHDAL
                     SWQNPHAISVAPMLPANGPVLKGLLLPPEYAISDASGYGIEPFLARLEARLREGLSLV
                     QVREKALSAAALRDFAARAVSLCRRYGARVLINADTQLSREVGADGVHLTSAQLGTLT
                     ERPAVSWCGASCHSAEELRRAERLGADFAVLGPVRATPTHPGVATLGWAGFRAAAEGA
                     AIPVYALGGVVPRDLDDALAGGAHGLAMVRGAWSA"
     gene            complement(18996..19184)
                     /locus_tag="VI319_04095"
     CDS             complement(18996..19184)
                     /locus_tag="VI319_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY6863058.1"
                     /translation="MGKVRTVACPRCGKQVAWDPGNAYRPFCSERCKIFDLGAWATEQ
                     YRVPVEDEKDEPDSDENR"
     gene            complement(19193..>19475)
                     /gene="zapD"
                     /locus_tag="VI319_04100"
     CDS             complement(19193..>19475)
                     /gene="zapD"
                     /locus_tag="VI319_04100"
                     /inference="COORDINATES: protein motif:HMM:NF018740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEY6863059.1"
                     /translation="LLPLRDGSNIVLRLLREGGKPTQLVAHQGTFSQMLGGKVSQMVR
                     IRLPRESQFIPEISANRYALNIRFTMFGTDPRPRICESDVEFELTFCNL"
BASE COUNT         3089 a         6557 c         6806 g         3023 t
ORIGIN      
        1 tgcaggtcgg gtacttcaag tggtcgggcg gcaagcggat cttccgcatg gcgcccgttc
       61 accaccactt cgagctcaaa ggctggcatg aaaaccaggt agttgttcgc gcctggatca
      121 ttacgatgct gctagtactt gccggtctct catcgttgaa actgcggtga atgtccgaga
      181 caaaaacata ctggtgctcg ggttgggaga cacgggcctc tccatggcgc actggctgac
      241 gcgacgccag gcgaaggtcc gggtcgccga ctcccgcgag gcgccgccag gcctggacgc
      301 gctgggccgc tcgctgcccg ggctcgcgcc ggtgctcggg ccgttccgcg aggagagctt
      361 cgccggcgtg gacctgatcg ccgcgagccc cggggtgccg ctcgcggagc ctcgtctaca
      421 gcaggcgctg cgcgcgggcg tcgaggtcgt cggcgacatc gagctcttcg cccggtcgct
      481 ggaggccgcg cgccggccgc tcgtcctcgg cgtgacgggc accaacggca agagcaccgt
      541 gactgcgctc gccggcgcca tggcgcgcgc cgcgggcagc gactgcgaag tcgccggcaa
      601 catcgggccg ccggtgctcg acgcgctgat gcgaagggag gacgaggcgc gtgccgcgaa
      661 gctctgggtg ctcgagctgt cgagcttcca gctcgagacg acgagctcgc tggatgcgag
      721 cgcggccgtc gtgctgaaca tcaccgagga ccatctcgac cgctatccgg gcatggccga
      781 atacgccgcg gcgaaggcgc gcatcttccg gggcgacggt gtgcaggtgc tgaaccgcaa
      841 cgatccgcgg acgctcgcga tggcgctgcc cggccgcacg gtgctgacct tcgggctgga
      901 tgcgccgccc ggtccgcgcg actatgggat cttggcgcgc tcggtggagg actggctcgc
      961 cgaaggtgcc gaagcgctga tgccggtgaa ggctatgaag ctgccgggcc gtcacaacgc
     1021 cgccaacgcg ctcgccgcgc tcgcgctgtg ccgcgccgtc ggcctgccga tggcgccgct
     1081 gctcggcgcg ctgcgcgagt ttcgcggcct gccccatcgc gtcgagccgg tcggcgagat
     1141 cggcggcgtg cgctactacg acgattccaa gggcaccaac gtcggagcgt cggtggccgc
     1201 gatcgccggg ctcgcgcacg atcatcccgg gatcgtcctg attgccggcg gcgaaggaaa
     1261 aggccaggac ttcgcgccgc tgcgcgagcc gctcgcgcgc tcggcccgcg cggtggtact
     1321 catcggccgc gacggcccgc tgatcgagaa ggcgctgagc ggggcgggcg tacctctgcg
     1381 taaggcggcc ggcatgcagg aggcggtaac gctcgccgcc gcggaggcgc ggcccgggga
     1441 cgccgtgctg ctgtcgcccg cgtgcgcgag cttcgacatg ttccgcaact acgctcaccg
     1501 cggcgaggtg ttccgcgcga cggtggaagg gatggcccgt ggcgacgctc gttagcgcgg
     1561 gccggccgca ggcgcccgag tacgactcgg gcctgctctg ggccgccgtt ttgctcctcg
     1621 gcttcgggct cgtgatggtg tattccgcgt cgatcgcgat ggcggaggcg agcgcgttca
     1681 cgggcggccg cgcgacgtat tttctcatgc ggcacgccgt cttcctcgtg ttcgccgtga
     1741 tcgccgggat gctcgcgttc caggtgccga tgcgcctctg gcagcgcgcc gcgcccgcgc
     1801 tgttccttgc ctcggcggcg atgctcgccc tcgtgctcgt cccgggcatc gggcgcgagg
     1861 tgaacggcgc gaggcgctgg atcgggctcg ggcccgcggg gttgcagccc tcggagttca
     1921 tgaagctctt cgcggtcctc tatgcggccg attacacggt gcgcaagtcg gcgctgatgc
     1981 agagcttcaa gcagggcctg ttgcccatgc tcgccgtgat gctcgtggtc ggcgggctgc
     2041 tcctgcagga gccggacttc ggagccttcg tcgtgatcgc cgcgatcgcc accgggatcc
     2101 tgttcctcgg cggcatgaac gggaaatggt tcgcgggatt gctcgtcatg ctggcgctcg
     2161 gcttcgcggg actcgtgctg tcgtctcctt accgcatgca gcgcatcttc ggcttcatgg
     2221 atccgtggtc cgatcccttc gggcgcggct atcagctctc gcacgcgctg atcgcgttcg
     2281 gccgcggcga gtggttcggc gtcgggctgg gaggcagcgt cgagaagctg ctctacctgc
     2341 cggaagcgca caccgacttc ctgctcgcgg tgatcgccga ggagctcggc ttcgcgggcg
     2401 tgctcgcggt catcgtcctg ttttcctggc tcgtgctgcg cgcgttcgcg atcggccgcc
     2461 aggcggtggc gctcgagcgt ccgtattggg cgctcgcggc gcagggcatc ggcatctgga
     2521 tcggcgcgca gacgctgatc aacatgggcg tcaacatggg cgttctgccg accaagggac
     2581 tgacgctgcc cctgatgagc ttcggcggct cgggcctcgt cgccaactgc atcgccctcg
     2641 cggtgctcct caggatcgac tgggagtcgc ggcagatgat gcggggacgt cccgggtgag
     2701 cgccacgcca tgtcgaggac cctcatgatc atggcaggcg gcaccggcgg acacatcttt
     2761 ccagcgctcg cggtcgccga tcgcatgcgc gaggccggct ggcgcgtcgt ctggctcggc
     2821 gcgcgcaccg ggatggaggc gcgcctcgtg ccggcgcgcg gctacgagat ggcctgggtc
     2881 gccttctccg gcgtgcgcgg caaggggccg ctcgcgctcg cgctgctgcc gacgcggctg
     2941 ctggtggcgt tctggcagag cgcgcgcgcg atcttctccc ggcgccccga cgtcgtgctc
     3001 ggcatgggcg gctacgtctc gtttcccggc ggcatgatgg cggcgctgct cggccggccg
     3061 ctcgtcatcc acgagcagaa ttcgatcgcc gggctcgcga acaaggtgct cgcccgcgtc
     3121 gccgaccgcg tgctcgcgag cttcccgggc gcgctgccgg gggcggtggt caccggaaac
     3181 ccggtgcgtc ccgagatcgg cgcgctcgag gggccggaga tccggtacgc aggccgctcg
     3241 gggccgctgc gcctgctcgt cgtcggcggc agtctcgggg cgaaggccct caacgatgcc
     3301 gttccggcgg caatggcgct cgtgccgcgc gagcggcgcc cggtgatccg gcaccaggcc
     3361 ggcgcgaagc acatcgaggc gcttcgcgcg gcgtacgccg ccgcgggcgt cgaggcggag
     3421 accgtgccgt tcatcgacga catggcgaag gcctacgccg aggcggacct ggtcgtgtgc
     3481 cgcgccggcg cgaccagcat cgccgagatc gcggccgccg gcgtggcgag cgtgctggtt
     3541 ccgtttccgt tcgcggtcga cgatcaccag accacgaacg cgcgcctgct cgcggacgcg
     3601 aacgccgggg tgctgctgcc gcaatcgcag ctcacgcccg agcgcctgtc cgccctggtg
     3661 ctgtcgttcg accgcgggcg gctgctcgac atggcgcgcc gcgcgcgcgc gctcggccgc
     3721 ccggacgcgg cgcgcgcggt cgcgcaagcg tgcgaggcgc tcgcccgatg aagtacaagg
     3781 tcaagcgcgt gcacttcgtc ggcatcggcg gggcgggcat gagcggcatc gccgaggttc
     3841 tgctcaagct cggctaccag gtgagcggct ccgacgtggc cgagagcgcc accacgcagc
     3901 gcctcgagga gcgcggggcc aaggtccgga tcgggcacga ccccgatcac gtcagcggcg
     3961 ccgacgtcgt ggtgatctcc accgcgatca agcacgacaa tcccgagatc gccgcagcgc
     4021 gtgccggcca cattcccgtg gtgccgcgcg cgatgatgct cgccgagctg atgcgtctca
     4081 agcaggggat cgcggtcgcc ggcacgcacg gcaagacgac gacgaccagc ctgatcgcga
     4141 gcgtgctggc cgaggctggc gaggacccga ccttcgtcat cggcgggcgc ctgaacagcg
     4201 cgggctcgca cgcgaagctc ggcagcggcg acttcctcgt ggtcgaagcc gacgagtcgg
     4261 atgcttcctt cctgcacctg cagccggtgg tcgccgtcgt gaccaacatc gacgtcgacc
     4321 acatggagac ctacaaccac gatttctccg ggctgaagcg caccttcatc gatttcctgc
     4381 agcggctccc gttctacggc gccgcggtcg tctgcctcga cgacccgggc gtgagcgaga
     4441 tcatcccgct ggtgagccgg cgactcatca cctacggctt gcgcagcgac gccgacatcc
     4501 gcgcgctcgg cgtggagcac gtcgccggaa agatgcgctt tcgcgcggag cgcgcgcccc
     4561 ggctgctgcg gcgcgggcag aaagcggcgc tcgagatcca gctcaacgcc gccggcgctc
     4621 acaacgtgct caacgcgctc gcggcgatcg gcgtggcgca ggagatcggc ctcgacgatg
     4681 gcgccgtggt tcgcgcgctg gcgaatttcc agggcgtggg ccgccgcctt cagcgctacg
     4741 gcgatgtgac gctgcggcgc ggctgcaccg tgaccgtggt cgacgattac ggccaccacc
     4801 cggcggagat gcgcgtcacc ctcgaggccc tgcgcggagc gtttccgggg cggcgcctgc
     4861 tgctcgcgtt ccagccgcac cgctacacgc gcacgcgcga tctcttcggc agcttcgtcg
     4921 aggtgctctc gggcgtcgac gagctgctgc tcgccgacgt ctatcccgcg ggcgaagcgg
     4981 ccatcgccgg cgccgacggc aaggcgctgt accaggcggt gcggcgcttc gacgcggtca
     5041 aggcctcgtt cgtcggaaac atctcggaca tggcggaggc gatccgcaag gcggctcaga
     5101 acggagacgt cgtcgtgacc atgggggcgg gctcgatcgg gacggtgccc gcggcactgc
     5161 tgaagacggc gcgatgaaga tgaccgagcc tatccacaag cccgccgcag ccctgcgcgg
     5221 tacgctgcgc ctcaacgagc cgatggcgcg ccacgtgagc tggcgcgcgg gtggcgccgc
     5281 ggcgcgcgcg tacctgcccg cggacactgc agatctagcc ctcttcctgc gcaccttgcc
     5341 cgccggcgag ccggtcacgt tcgtcgggct cggcagcaac ctgctcgtgc gcgacggcgg
     5401 ctaccgcggc accgtcgtcc tgctgcacaa cacgcgcgca gcggtccggc tcgacggtcc
     5461 gctggtctac gcggaggccg gcgtcgctgc gcccaaggtc gcccggttcg cgggcacgca
     5521 cgcgctcgaa ggcgcggagt tcctcgccgg gatcccgggg accgtgggcg gcgcgctcgc
     5581 gatgaacgcc ggctgctacg gcagcgaaac ctgggacatc gtggcgcggg tgcagacggt
     5641 ctcgcgctcg ggagacttgc gcgagcacgg ccgcgagcag ttcgacatcg ggtaccggca
     5701 cgtcgcgctg cgcggcgcgc ggctcggcga ggacgagtgg ttcgtcgccg gctggttcgc
     5761 gctgcgcgcc ggcgacggcg cgcgcgcacg cgcacgcgtg aagcagctgc tcggccgccg
     5821 catcgcgacg cagccgctcg gcctgccgaa cgccggctcg gtgttccgca atccgcccgg
     5881 cgaccacgcg gcccgcctca tcgaggtctg cggactcaaa ggctacgccg cgggcggagc
     5941 gcgcatctcg gagaagcacg cgaacttcat cgtcaatccc gagggcggcg cgcgcgccgc
     6001 cgatatcgag cgcctgatcg agcacgcgcg cgccgaggtg aagcgccggc acggcgtcga
     6061 cctggtgccc gaagtgagaa tcatcggaga agcgacatga aacccgccga tttcggaaag
     6121 gtcgccgtgc tgctcggcgg caagtcggcc gagcgcgaag tctcactcaa cagcgggcgt
     6181 atggtgctcg ccgcactgcg cgcaaagggc gtcgacgcgc atccgttcga tccccgcgac
     6241 cgcgggctgg accagctcat tgccgcgcgc ttcgaccgcg tgttcatcgc cctccatggc
     6301 cgctacggcg aggatgggac cctgcagggg gcgctcgagc tgctcggcct gccttacacg
     6361 ggatcggggg tgctcgcgag cgctctcgcg atggacaagt ggcgcaccaa gctggtctgg
     6421 cagtcgtgcg gggtgccgac ccctcactcg gaggtgctca ccccgcaaac ggatttcgag
     6481 gccgtcgcga accgggtcgg ccttccactc atggtgaagc ccgcgaacga aggctcgtcg
     6541 atcggcatga gcaaggtccg gacggcggcg gcgctcgccg acgcgtacgc gctcgccgcg
     6601 aaatacgact cgctcgtcat cgccgagagc ttcgtcgagg gcgtggagct caccggcgct
     6661 gttctcggcg atacgccgct gccgctgatc cggctcgaga cgccgcacga tttctacgac
     6721 tacgacgcca agtacaaggc cgccgacacg cgctacatca ttccctgcgg gctgcccccc
     6781 gacgccgagc gtgtcatcca ggacgaggtg ctgcgcgcgt tccgggcggt cggctgcagc
     6841 ggctgggggc gggtcgatct cattctcgat cgctcgggca agccctactt cctcgaagtc
     6901 aacacgtcgc ccgggatgac cgaccactcg ctcgtcccga tggcggcgcg tcacgccggc
     6961 atcgcgttcg aggatctgtg cctgcggatt ctcgagctcg cgcgcgtcac gacgaaaacg
     7021 ggaggcgacg gtgtgggata gcccgcgcct gctcaacctc gcggccggag tcctgctcgc
     7081 ggccgcggtc gcgctcgtgc tcgcgggggc tgccgcctat gccatgcgct cgcccgcgtt
     7141 cgtgctcaac agcatccgcg tcgtcggcga cgttgaccat gtcgccgtgc cgcaggtgat
     7201 cgacgcgctc cagggccgcg tcagcggcac tttcttttcg gtcgacatcg acggcatccg
     7261 tgcgctgttc gagacgctgc cctgggtgcg gcgcgccgag gtgagacgcc tgtggcccga
     7321 cggcctcgag gtccgcatcg aggagcatgt gcccctcgcc cgctggggcc aggagcgcga
     7381 cggccggctc gtgaacagct acggcgagcc tttccgcggc cgtgtcgaag gagcgctccc
     7441 cgcattcttc ggaccgccgg gcagcgaagg catgctcgcg cgtcgctatg ccgacttccg
     7501 gcgcgtgctc gcgccgctct cgatcgagcc ggcctcggtg cttctgagcg cgcgctacgc
     7561 ctggcagctg cagctgtcga gcgggctcat cgtcaagctc ggacgcgacc gcgacaagga
     7621 cgatgcggtc cagcggctcg cacgcttcgt cgccgtgtac ccgtacaccc tcgggaaggc
     7681 gcagcgccgg ttcgagtacg tcgacttgcg ctacccgaac ggattcgcgg tgcgcgcggc
     7741 ggacgcgcgc tcgctgcacg agcggctgcc gaggacctga tgcccaaaga caacaagaac
     7801 ctcgtggtcg gactcgacat cgggacctcc aaggtggtcg cgatggtcgc ggagctgatg
     7861 cccgacggcc gcatggatgt gatcggcatg ggaagccacg agtcgaaagg cctgaagaaa
     7921 ggcgtcgtgg tgaacatcga gtcgacggtc agcgccatcc agcgcgcgct cgaggaagcc
     7981 gagctgatgg ccgactgcaa gatccagcgc gtcttcacgg gcatcgcggg cagccacatc
     8041 aagagcttca actcgaccgg gatggtcgcg atcaaggaga aggaggtcac gccgctcgac
     8101 gtcgaccgcg ccatcgagac ggcgcgcgcg atgccgatcc cgaccgacca gcaggtcctg
     8161 cacatcctca cgcaggaatt catcatcgac ggccaggacg gcgtgcgcga gccgctcggc
     8221 atgagcggcg tgcgcctcga ggtcaaggtg cacatcgtga ccggcgccgt gtcggccgcg
     8281 cagaacatcg tgaagtgcgt gcgccgctgc ggcctcgagg tgagcgacct gatcctgcag
     8341 ccgctcgcct ccagcctcgc ggtgctcacc gaggacgaga aggagctcgg cgtgtgcctg
     8401 gtcgacatcg gcggcggcac gaccgacatc gccgtgttcc gagaaggcgc gatacgccac
     8461 accgcggtca tcccgatcgc gggcgaccag atcacgaacg acgtcgcgat ggcgctgcgg
     8521 acgccgaccg ctgaggccga ggagctgaag gtgcggcacg gctgcgcgct gcggcagctc
     8581 gccgaggccg gccagatgat cgaggtgccg ggcgtcggcg atcggccgcc gaggatgctg
     8641 tcgcgccaga cgctcgccga ggtcatcgaa ccgcgcgtcg aggagctcta ctcgctgatc
     8701 cagcaggtgc tgcgcgactc ggggtacgag gagctgcttt cgtcgggcct ggtgctgacc
     8761 ggcggctcgg cggtcatgca gggcatggtc gagctcggcg aagagatctt ccatatgccg
     8821 gtgcggctcg gggtgccgag gtattcgggc gggctgtccg acgtcgtgcg cagcccgcga
     8881 tacgccacgg cggtggggct gctcatggaa ggcgtgacgc agacccggcg cgggcaggtc
     8941 gcccgccagg gcgggtcgtt caagcaggtg atgggaagga tgcgcgaatg ggtgcagcgg
     9001 aatttctagg agcgggaggg aagcggatgt ttcactttct gatgagtcaa ggaggcaggc
     9061 atgtttgaaa tcgtcgaaaa cagctcgggc ggcaccgtga tcaaggtgat cggcgtcggc
     9121 ggcgcaggcg gcaacgcggt cgatcacatg atccgcaacg gcgtgtgcgg cgtggaattc
     9181 atcgccgcga acacggacgc gcaggcgctg tcgcgaggtc ttgccaagca gcagctgcag
     9241 ctcggggcca cgggcctggg cgcgggcgcg aagcccgagg ccgggcgcgc cgctgcgctc
     9301 gaggcgcgcg accagctcgc ggagtcgctg cggggcgcgc acatggtgtt catcaccgcc
     9361 ggcatgggcg gtggcacggg cacgggggcg gctccggtcg tcgcggaagt cgccaaggag
     9421 atgggcattc tcaccgtggc cgtggtcacc aagccgttct cgttcgaggg cagccggcgc
     9481 atgcagtccg ccgaggccgg aatcgaggag ctgtcgcagc atgtcgactc ggtgatcgtg
     9541 atcctgaacg agaagctcga ggaggtcctc ggcgacgaca tctcgatgga ggaggccttc
     9601 ggcacggccg acagcgtgct caagaacgcg gtggccggga tctccgagat catcaacgtc
     9661 ccgggcctga tcaacgtgga cttccaggac gtgcgcacgg tgatggccga gcagggcatg
     9721 gcgatgatgg gatccgcggc gtcgtcgggc gtcgaccggg cgcgcatcgc ggccgagcag
     9781 gcggtcgcgt gtccgctgct cgagggcgtc aacctctccg gagcacgcgg cgtcgtcgtg
     9841 aacatcacgg ccgacaagtc gagcctcaag ctgcgcgaga ccaaggaggt gatgaacacg
     9901 atccgcggct tcgccgccga ggacgcgaca atcatcttcg gagcggtgta cgacgacgcg
     9961 atgggtgatg agcttcgcgt gaccgtcgtc gcgacgggcc tcgggcagcc gcaggccgct
    10021 cgccagccca agcctcaact cgtgcagcgg accgggacgg acaacctgcc cgtggctgga
    10081 gtcgactacc gggcgctcga cgagatgccc gcggtcatcc gcaagaaccg cagctccacg
    10141 atcgaggcgc tgcagaacag cggcgtcgac aagtacgaca ttccggcgtt cctgaggaag
    10201 caggccgact gacgaaccga cagggagact cacccatgag catcgcaaag cggcccgcgc
    10261 ttccgcccta tcgtcgcaag cccgccctgg cgaggccccg gctgcgcgtg tcggtgccgg
    10321 tcgagccggt cctcgttcgc accgtcgcgt tcatgccggc gtaccagcgc aagcgtattc
    10381 attgaataag gagcgcaaaa tcacgtcctg acgaggggct tccgggctac aatcgatgtc
    10441 cgtcccgctc catacaagcc acgcgatgtt caggcagcgc accctcaaat cggcgattcg
    10501 cgccaccggc gtgggtctgc acacgggtga aaaagtcgcg atgacgctga gacccgcgcg
    10561 ccccaacacc gggatcgtgt tccgccgtgt cgacacgccc aagcccgtgg acctgcgcgc
    10621 cgaggcgttc cgcgtcggtg acacccgcct cagtacgacg ctcgaggacg gcgaggtgaa
    10681 ggtcgcgacc atcgagcacc tcatgtccgc gttcgccggg ctgggtgtcg acaacgccta
    10741 cgtcgacctc tcgggctcgg aagtgccgat catggatggc agcgccgctc cattcgtgtt
    10801 tctcatccag tcggcgggca tcgaagagca gccggccgcc aagcggttct tccgcatcaa
    10861 gcgcccggtc gaggtgcgcg acggcgacaa gtgggcgcgt ttcgagcctt acgagggcct
    10921 gaagctctca ttctcgatcg tgttcgatca cccggtattc gagaagtccg cgcaggaggc
    10981 gagcgtcgac tttgccgaga cctcctacgt caaggaagtg gcgcgcgccc gcacgttcgg
    11041 cttcatgcag gacgtcgaag caatgcgctc gagcggtctc gcgcagggcg ggagcctcga
    11101 caacgcgatc gtcatggacg aataccgcgt cctgaacgcc gagggacttc gtttcggcga
    11161 cgagttcgtc aagcacaagc ttctggacgc catcggtgat ctgtatctgc tagggtatcc
    11221 gctcatcggc gcctatgccg cgcacaagtc ggggcatggg ctaaacaacc gcctgctgcg
    11281 ggagacgatg gcacgcaagg aggcgtggga gctcgtgagc ttcgagcgcg ccgaggacgc
    11341 gcccgcggcc ctgagccgcc tgtttgcttt gcccgcatag cgcgccccgt gctggcgctg
    11401 cgcgtcgtcg caatcctggt cgtgctcggc gcgctgtctg cgttggcact ctatctcttc
    11461 actcgcgacc gccgctatct acgcttcgcc gtgcgactgc tccaggtcgc tgtgatattt
    11521 gccctgtttt tgtttggctt attggcgctc gaacgagcgg tggcgcttgc ctagtcaggg
    11581 cttgctgacg tcatggcggg cgagcgccgc gacgcattgt ttcagcgggg aatcagggag
    11641 ttgggccgca agctccgcga ggctggcgac cgcggagccg ctcagctttg ccgccttctc
    11701 aggtttttcc tgagccgctt caggagcttg cacgccgata tccatttcgg taacctgaac
    11761 tccacggtcc gaaaactgct cgcgtatcgt cggagaaagc agcttgagtt tcgcggcgac
    11821 cgcgttgttt tcggcgaaaa gaacgagctt cccctgcttg tagttagcga ccgtgcaggc
    11881 tcgcgacaac gcaccgggaa gtaggctcgc caggatctga cgaagttcga gcaggcgccg
    11941 ggcctgcggc atcaatgctg ggatgccgtc ggactccgaa agcagggcgc ttggcttgtg
    12001 tggatgcatg gtcatccgcc gagtagggag aagcaacatg cacattattc tggtttccaa
    12061 ccggctcgcc aaggcgagat cggtgacact gacgatggcg cacctggtcg ccggcgcggc
    12121 gtgcttcgcc acagtcatcc tcggcctcgc cgccgccatt ttctgggttt ccgtgcgcca
    12181 cgccgacgag ttcaacatcc cgcagctgca atcgctcgtg ctctccgccc agcaacagga
    12241 gacgcagaag acgcgcgact tcctgcgcga gaacctcaac gcgatggccg tcaagctcgg
    12301 cgagatgcag gcacagctca cgcggatcga cgcgctgggc gagcgcgttt ctgccctggc
    12361 gggattcaag ccgcaggaat tccgcttcag cgaagcaccc ggccgcggcg gcgccgtgtc
    12421 gagcattccg ccgcagaatc tctcgatggt ggagttcagt cagaagctcg accagctctc
    12481 caagcgcatg gagaaccgca ccgattacct cggcatcctc gagtcgcagc tgttcgacac
    12541 caagatgaag aagaagctga tgcccacgat tccgccggtg gacggtagct ggaacgcctc
    12601 gagcttcggc tggcgcatcg atccattcac gggaatgcac gcgatgcacg agggcatcga
    12661 cttcctggcc gagccgggca cgcccgtgtt cgcttccgcg ggcggtgtgg tgaccttcgc
    12721 cgactttcat ccgcagtacg gctacatggt cgagatcgac cacggcaacg acttcacgac
    12781 gcgctatgcg cacaattcca agctgctcgt caaagtcggc gacctggtgc accgcggccg
    12841 caagatcgcc gaatcaggat cgaccggccg ctcgaccggc ccgcacgtgc acttcgaggt
    12901 gcgctacaag ggcgtggcgc aggatcccaa ccgtttcctc caggcctcgg cgcgccagga
    12961 gcacaaccgg gtgctccagg cgtcggcgcg cgcgcgctaa cggccctgcc cggcccgctc
    13021 gggcttcgag cgcttcccgt agtccgtaat gagcccggtc cgccccgagg gcggacgcgc
    13081 gcggcatgct aaaatttccc cttttttgcg cgccccgcat ctgcttgaaa tcccgttgaa
    13141 atgatttccc ggttcctcaa ggccatcttc ggcagccgca acgaccggct gctgaagcag
    13201 tacgcgaagt cggtgagggc gatcaacgcc ctggagccgg ccgtgtcgac gctctcggac
    13261 accgaactcg ccgcgaaaac gcccgagctg cgcgagcgcg tggcgaagct gctcgaggcc
    13321 gatacccagg aggaccgcaa ggcggccgag cgcgcggcgc tgcacgaggt ccttcccgag
    13381 gcgttcgcgg tcgttcgcga agccgccaag cgcacgctca acatgcgcca tttcgacgtg
    13441 cagctgatcg gcggcatggt cctgcacgac ggcaagatcg ccgagatgcg aaccggcgag
    13501 ggcaagacgc tggtcgcgac acttcccgcg tacctgaatg cgctcacccg caagggcgtg
    13561 cacatcgtca cggtgaacga ctatctggcg agccgcgacg ccgaatggat gggccgtgtc
    13621 tacaacttcc tcgggctgtc ggtcggcgtg atcctcacgc agatgccgca cgaggacaag
    13681 cagaaggcct acggcgccga catcacctac gggaccaaca acgagttcgg gttcgactac
    13741 ctgcgcgaca acatggcgat gtcgctcgcc gacaagttcc agcgcgggct gaactacgcg
    13801 atcgtcgacg aggtcgactc gatcctcatc gacgaggcgc gcacgccgct catcatctcg
    13861 ggacaggccg aggaccggac cgagatctac catcgtatga acgaggtggc gccgctgctg
    13921 aagcgccagg agaaggaggg cgctcccggc gatttcctcg tcgacgagaa gaaccacacg
    13981 gtactgctct cggaagaagg gcacgagcac gccgagagct tcctggcgcg cgccggcctg
    14041 ctgcccgagg gccgcagcct gtacgagccc gcgtacgtga acctgatgca tcacatgaac
    14101 gcggcgctgc gtgcccatca tctcttcttc cgcgaccgtc attacgtggt gcaggacggc
    14161 gaggtcgtga tcgtcgacga gtttaccggc cggctgatgc cgggccggcg ctggtccgac
    14221 ggcctgcacc aggcgatcga ggcgaaggag aacgtctcga tccagagcga gaaccagacg
    14281 ctcgcctcga gcacgttcca gaactacttc cgcatgtacg gcaagctcgc cggcatgacg
    14341 ggcaccgcgg acaccgaggc gtacgaattc caggagatct acggcctcga ggtcgtggtg
    14401 atcccgacgc acaagaagat ggttcgcatc gacaacctcg accaggtgtt ccgcacggcg
    14461 aaggagaagt acaaggccgt catcgacgac atcaaggact gctacgagcg cggccagccg
    14521 gcgctggtcg gcacgacctc ggtcgagaat tccgagctcc tgtccgggat gctgcagaag
    14581 cagggcatcc cgcaccaggt gctgaacgcc aagcagcacg cgcgagaggc cgagatcgtg
    14641 gcccaggccg gtcggcccag gatgatcacg atcgcgacca acatggcggg ccgcggtacc
    14701 gacatcgtcc tcggcggcaa cgccgagaag cagatcgatc tgctgcgcca gaaccccgat
    14761 ctgccggacg acgatcgcga agcgcgcatc agcaagctgc gcgacgaatg gcagtcgctg
    14821 cacgagcagg tggtggccgc gggcggactg cacatcgtcg gcaccgagcg gcacgagtcg
    14881 cgccgcatcg acaaccagct gcgcggccgc tcgggtcgcc agggcgaccc ggggtcgtcg
    14941 cgcttctatc tcgcgctcga ggacccgctg ctgcgcatct tcgcgggcga ccgcgtcaac
    15001 gccatcatgc agcgcctcaa gatgcccgag ggcgaggcga tcgagcacgt gctcgtgacg
    15061 cgctcgatcg aatcggcgca gcgcaaggtc gaggcgcaca acttcgatat ccgcaagcag
    15121 ctgctcgaat acgacaacgt ggccaacgag cagcggcaga atatctacga cttgcgcaac
    15181 ggcgttctcg agtccgagaa cgtgtcggat cgcatccgcg cgctgcgcga aggcgtcgtc
    15241 accgaccttt tccgcgcata cgttccgcag gaaagcgtcg aggagcaatg ggatatcgcc
    15301 ggcctcgaga aggcctatgt ctccgagctg cgcgtcgagg tgccgctcgc gaagtggctc
    15361 gagcaggatc cgagcctcga cgacgaagcc ctgctcgggc gcgccgtcga ggcggcgaac
    15421 cgggcgtacg actccaagat gcagggcgcg caggccgaca gcgtgcacca cctggagcgg
    15481 tgggtgctgc tgtccacgct cgacacgcac tggcgcgagc acctggcggc gctcgaccat
    15541 ctgcgccagg gcatccacct gcgagggtac gcgcagaaga acccgaagca ggagtacaag
    15601 cgcgaggcct tcgagctctt cggcaacctg ctcgagacga tcaagctcga ggtcacgcgg
    15661 accctcatgt ccgtgcggcc gccgaccgag gaggagctgc ggcaggttga agagcagcag
    15721 cgcacccccg ccgtggagaa cgtgcaatac cagcacgccg gctacgaaga ggctctcgcc
    15781 ggccccgcgc cgggagaaga agagcaggcg ggagacgccg cgcccaagac cgcgcccttc
    15841 gtgcggcaaa tgggcaagat cggccgcaac gatccctgcc cctgcggctc gggcaagaaa
    15901 tacaagcact gccacggcaa gctggcctga tggccgtcaa tctcaaggca cccgatccgg
    15961 ccgatctgca cccggttcgc ggagtgaggc tgggcgtcgc ccaggccggg atccgtaaag
    16021 ccaaccgcac cgacctgctt ctggtcgcgc tcgacccggg cgcgagcgcc gcgggcgtgt
    16081 tcacgcgcaa tcgcttctgc gccgcgccgg tgctggtcgc gaaggagcac ctcgacgccg
    16141 gcacgtccgt ccgtgcgctg gtcgtgaaca cgggcgtcgc gaatgcgggc acgggagaga
    16201 cgggcatcgc gcgcgcccgg gagacctgcc gcgaagcggc acggctgctc ggctgcgacg
    16261 cgtcccaggt cctcccgttc tccaccggcg tgatcatgga gccgctcccc gcggacagga
    16321 tcgcggcggc gcttccgaga tgcgtcgccg gcctgtccga aagccggtgg tccgaggccg
    16381 cggccgcgat catgaccacc gataccgtgc cgaaagcggc ctcgcggcgc gtgcgcgcgg
    16441 gtgatgcgag catcggcttc accggcattg ccaaaggcgc cggcatgatc catcccgaca
    16501 tggccaccat gctcgccttc attgcgttcg acgcgcgggt cgccccagcg ctgctgcagc
    16561 gcatggtcga gcgcgccgca gcagcctcgt tcaatcgcat cacggtggac ggcgatacct
    16621 cgaccaacga cgcattcatc gtgatggcga ccgggaaggc ggggccgccg atcgagagcg
    16681 agcgcgatcc gggcttcgcg tccgtgtacg aggggctcga agacgtcgcg aagcgtctcg
    16741 cgcaggcgat cgtccgcgac ggggaaggcg cgaccaagtt catcacggtc cgcatcgagc
    16801 agggcgccga cgcagccgag tgcgaccgcg tggcctacgc gatcgcgcat tcgccgctcg
    16861 tgaagacggc gttcttcgct tccgacccga acctcggcag gatcctggcg gcgatcgggt
    16921 atgccggcat cgccgacctc gacgtctccc gggtcgacgt ctacctcgac gacgtgctgg
    16981 tcgccaaggg aggagctcgc gcggcgggct accgcgagga ggacggacag cgcgttatga
    17041 agaaagccga gatcgccgtg cgtgtcgcgc tcggccgggg tgccgccggg accgagatct
    17101 ggacgtgcga cctctcgcac gactatgtct ccatcaacgc cgattaccgc agctgacgcg
    17161 tggcgaaagg gggaaaccgc atgtcggacg tcgatcggct gatgcagcag gggcacgagc
    17221 tgctcgcccg catcgaggcg ttgctcccgg gacgtccggc gcccgtcgac tgggacgcga
    17281 gcatcgcctt ccgttggcgc aagcaggcgc agcgcggcgt gctccagccg gtcgcgaatc
    17341 tgcacaagat ccgcctgtcc gacctgcagg gcgtcgatcg tcagaaggcg ctgatcgagc
    17401 agaatacccg ccagttcgtt gagggccgcc ccgcaaacaa tgtgctgctg acgggcgcgc
    17461 gtggcaccgg gaaatcctcg ctcgtgaagg cgatgctgaa caagttccac aagcaggggc
    17521 tgcgcctgat cgaggtggaa aagcacgacc tgctcgacct gcccgagatc gtggaccagg
    17581 tcgcaaagcg gcgcgagcgc ttcgtcatct tctgcgacga tctgtcgttc gaggcctcgg
    17641 agcccggcta cctcgccctc aaggtcgtac tcgacggatc gattgctgcg ccctcggaca
    17701 acgtgttgat ctacgcgacg tccaaccgca ggcatctcat gccggagtac atgcaggaga
    17761 acctcgagac caagcaccgc ggcgaggaga tccatccggg cgaaaccgtg gaagagaaga
    17821 tctcgctgtc ggagcgcttc gggctttgga tctcgttcta cccgttcgac caggacgggt
    17881 acctcggcat cgtcgcgcac tggctcgagg agctcggttg tcccggcgat ttcgaaaagg
    17941 cgcgccagga ggcgctgcaa tgggcgctcc tgcgcggctc gcgcagcggc cgcgtggcct
    18001 ggcagttcgc gcgcgactac gccggccgcc acggcaggac gcccaagaag ccgggatgac
    18061 gccgatcgag gtcgccgtcg cggtcttcgt gcgggccgac ggcgcggtcc tgctcgcgcg
    18121 acggcccgcg gggaaggtct acgcgggcta ctgggagttc ccgggcggga aggtcgagcc
    18181 cggggaagcc atggccgcgg cgctgcggcg cgagatccgc gaagagctcg gcgccgacgt
    18241 cgatcgcgcg tacccgtgga tcacgcgcgt cttcgtctac ccgcacgcga cggtgcgcct
    18301 gcatttccac cgcgtcgtcg cctggcgcgg cgagccccgg gcgctcgagc acgacgccct
    18361 gtcctggcag aacccgcacg ccatatcggt cgcgccgatg ctgcccgcca acgggccggt
    18421 cctcaaagga ttgctgctgc cgcccgagta cgcgatcagc gacgcgtcgg gatacggcat
    18481 cgagccgttt ctcgcgcggc tggaggcccg cctgcgggag gggttgagcc tggtgcaggt
    18541 gcgcgagaag gcgctgagcg cggccgcgct gcgcgatttc gcggctcgcg ccgtatcgct
    18601 gtgccggcgc tacggtgcgc gcgtgctgat caacgccgac acgcagctct cgcgcgaggt
    18661 cggtgccgat ggcgtacatc tgacgagcgc ccagctgggg acattgaccg agcgccccgc
    18721 ggtgtcctgg tgcggcgcgt cgtgccattc ggcggaggag ctgcgccgcg ccgagcggct
    18781 gggcgccgac ttcgcggtgc tcgggccggt ccgcgcgacg ccgacgcatc ccggggttgc
    18841 gacgctcgga tgggccggct tccgcgcggc ggccgagggc gcggcgattc cggtgtacgc
    18901 gctcgggggc gtcgtaccgc gggacctcga cgacgcgctc gcgggcggcg cgcatgggct
    18961 cgccatggtt cgcggcgcgt ggtccgcgta gccgttcagc ggttctcgtc gctgtcgggc
    19021 tcgtcctttt cgtcctcgac cggaacgcgg tactgctcgg tcgcccaggc gccgagatcg
    19081 aagatcttgc agcgctccga gcagaacggg cggtacgcgt ttccgggatc ccacgcgacc
    19141 tgcttgccgc atcgcggaca cgcgacggtg cggaccttgc ccacgttcgc cctcagaggt
    19201 tgcagaaggt cagctcgaac tccacgtccg attcgcaaat ccgcgggcgc gggtcggtgc
    19261 cgaacatcgt gaagcgtatg ttgagcgcgt aacggttcgc gctgatctcg ggaatgaact
    19321 gcgattcgcg cggaagccgg atccgcacca tctgcgacac cttgccgccg agcatctgcg
    19381 agaacgtccc ctggtgcgcc acgagctggg tgggcttgcc accctcgcgc agcagtctga
    19441 ggacgatgtt cgagccgtcg cgcagcggca gcagc
//
LOCUS       DATDMW010000097        21428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2392769, whole genome shotgun sequence.
ACCESSION   DATDMW010000097 DATDMW010000000
VERSION     DATDMW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21428
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392769"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VI319_04105"
     CDS             complement(<1..241)
                     /locus_tag="VI319_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863060.1"
                     /translation="MSRLRRRRALQGAQESHGQGLEEHASIHERPFRRDLVERSEVLD
                     WIRRIDRKPLLLLDWPLGDTAASPLKAPRASKVCPT"
     gene            296..721
                     /gene="msrB"
                     /locus_tag="VI319_04110"
     CDS             296..721
                     /gene="msrB"
                     /locus_tag="VI319_04110"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY6863061.1"
                     /translation="MLADGADVAASAEPIERANAEWRKTLTPAQYDVLRQEGTERAGT
                     SPLDREKRPGVFACAGCGLPLFTSAMKFDSGTGWPSFFTTIPGMLATKRDFKLIVPRT
                     EYHCVRCGGHQGHVFNDGPQPTGLRYCNNGVALRFIPKA"
     gene            complement(705..1580)
                     /locus_tag="VI319_04115"
     CDS             complement(705..1580)
                     /locus_tag="VI319_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6863062.1"
                     /translation="MKLRSVELELPRADAAVQFLTGPWGLIEAGKSGGTVYLRGTGEH
                     PYVMAIAEAPGPALVSVTFAGAPKELERVKARAKKAGRAITPYAPRPGEPGAPKGFTL
                     AGPEGQVFRFVSDKKPATRIKDADRPIGLTHVPISAADADACTKFALEVLGFKLSDRT
                     KMMDFIRCDSTHHSLAYVRTGAPALHHMAFEMQDLDAVMRGVGRLKDAGLDPVWGPGR
                     HGPGNNVFAYYVSPFGAVVEYTSEVERVGPGYKVGGPEDWKWPPGRIDHWGLSKRDET
                     RLRAAETSFRFRPSG"
     gene            1641..2630
                     /locus_tag="VI319_04120"
     CDS             1641..2630
                     /locus_tag="VI319_04120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY6863063.1"
                     /translation="MSDDNAPAYAGLTPERVLDALDAVGFRGDGRLLALNSYENRVYQ
                     VWLEEGGSVVAKFYRPERWSDAQIHEEHAFASELADREIPAVPPIADARGATLHAHEG
                     FRFAVFPRKGGRAPELEDPAVLEWLGRFLGRIHAVGATRPFAHRPALDLETFGREPRD
                     WLLGHGFVPADLLEAWRAAVGQALDGVALCYERAGGVKTLRTHGDCHSGNVLWTDEGP
                     HFVDFDDARTAPAVQDLWMLLSGDRPAMTRQLGDLLAGYEDFMDFDRRELHLVEALRT
                     LRLVHYSAWIARRWSDPAFPAAFPWFGTQRYWQDRILELKEQIALMAEEPLHA"
     gene            2674..3516
                     /locus_tag="VI319_04125"
     CDS             2674..3516
                     /locus_tag="VI319_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6863064.1"
                     /translation="MPVAKVRGLNIQYRVVGERGPWVTLTTGGRRGHQEFVALAQKIA
                     DAGFRVLLHDRRNTGASDILIEGDETEEAIWADDLYELLRQLGAAPAFIGGSSSGART
                     SILFALRHPDAARGLLLLRVTGGAFAAKRLPEMYYDQFLRAAKEGGMAAVCATEQYRE
                     RFAENPANLERMKKTDVKQYVAVMTRWRDNFVAGASLPVMGVTEKELRSIRVPTIVIP
                     GNDRTHSSESARVAHRMIPGSELHALPITDQDVDLIPFPKWAPYEDEIARVFVDFMRR
                     NGAR"
     gene            complement(3526..4401)
                     /locus_tag="VI319_04130"
     CDS             complement(3526..4401)
                     /locus_tag="VI319_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY6863065.1"
                     /translation="MKAIKGIYRGSEPHWVGDGFPVHTVFSYQDLASELSPFLLLDHA
                     GPADFEPTARERGVGWHPHRGFETVSVAYEGEVDHEDSTGSRGTIGPGDVQWMTAGAG
                     VLHKEMHGRNFARSGGRFEMLQLWVNLPAASKMTEPRYQTLLAADIPVVKLPRDGGSV
                     RVIAGEYQGAKGPARTFTPINLLDVRLKGDHRVRLELREGWTAALFVVAGEVIVNGER
                     GARAGDLVVLGRENGKVEIEGAPDATVFVMNGEPIDEPIVGYGPFVMNSVEEIQQAFA
                     DFQAGRLGKVPETVA"
     gene            4612..4809
                     /locus_tag="VI319_04135"
     CDS             4612..4809
                     /locus_tag="VI319_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863066.1"
                     /translation="MSQKPNSLEPSRPAGRRRVVDRVRERALLRAMRGALRRSVVLLP
                     LTEEDDLLQRFLVDALGLVPA"
     gene            complement(4865..5062)
                     /locus_tag="VI319_04140"
     CDS             complement(4865..5062)
                     /locus_tag="VI319_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863067.1"
                     /translation="MDRTLLVEDVTWLRAMRDAQHSSIPLHVAMKLRLRGYAVPNGQT
                     YTITLKGRDELIDRERESLFG"
     gene            complement(5319..5717)
                     /locus_tag="VI319_04145"
     CDS             complement(5319..5717)
                     /locus_tag="VI319_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863068.1"
                     /translation="MGARILGLCLVAALAVAGCASREPAPDPRQAWLGTTYDEVVRAW
                     GTPVRSATLSDGRQAYTWAWEGSRPRTGVSPSVGIGVGSGGFGIGTGVIFGSGGSEQV
                     QCERTLIFEAGHVAEQNWQGPPEVCDRFGR"
     gene            complement(5749..6639)
                     /locus_tag="VI319_04150"
     CDS             complement(5749..6639)
                     /locus_tag="VI319_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY6863069.1"
                     /translation="MIELVIEARRRDLGGFEVARVLPHAKRRMVGPFIFFDHMGPVEL
                     PRGVPRTVDVRPHPHIGLSTVTYLFEGEIMHRDSVGSEQAIRPGEVNWMTAGRGITHS
                     ERFERARAEGGRLHGIQAWVALPREAEESEPAFAHHGPGDLPFYEGGGLAARLLAGEA
                     FGARAAVRTHSPMFYVHWELAAGAKAQLPAQYPERAAYIVQGEVEVAGERHSAGRMLV
                     LAPGQPVVFEARAPSVLMLLGGEPVGERYIEWNFVASSRERIERAKADWRAGRMKLPE
                     RDDREFIPLPADAPPPEPMS"
     gene            6697..7842
                     /locus_tag="VI319_04155"
     CDS             6697..7842
                     /locus_tag="VI319_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY6863070.1"
                     /translation="MSELDTLRTDVVGSLLRPADWREARTRFERGEIDAGAFAAIERE
                     CVRRAIALQESAGLGVVTDGELSRLNFQDSFGMAVSGYDAAPDRLHGYERRTDGAVPL
                     KRWDMPDVAERGTPVSHRRPVVARLALVRNVPLEEYERARPLAKRPLKVSLIGPDRVL
                     QRFDHAGSKRVYASVDDFGADVVAVQRRMIGGLVDAGCRYVHIDAPGLTAYVDEPSLA
                     AMRARGEDPAQNLARGLAAEAAVVRGFDGVTFGLHLCRGNQRSMWHREGAYDAIAEQM
                     FGTLPHRRFLLEYDTPRAGSFAPLRFVPKDRIIVLGLVSTKVPRLETVDELRRRIDEA
                     AKVVPLERLAISPQCGFGSDVAGNLVGEDDQKRKLERVAETARLVWG"
     gene            complement(7846..8343)
                     /locus_tag="VI319_04160"
     CDS             complement(7846..8343)
                     /locus_tag="VI319_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863071.1"
                     /translation="MPHNRRLLRLIAVAVLLAAPGARAADQGSSSFGLSLAPGRQLDQ
                     TYTVLSGRVGHYFADDFEGSLGLEAWRGKDPSIYKLVPEIRYTVPTNPRARPYGALFV
                     SRTFYDGLPDKNTFGARLGFAFTINRGASLGVGIVHERISGCDEGTYRHCSDTWPEVG
                     LSFAY"
     gene            8455..9087
                     /locus_tag="VI319_04165"
     CDS             8455..9087
                     /locus_tag="VI319_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY6863072.1"
                     /translation="MPAEPPKHFSMIRGLHLADAFTLANAACGVAAVFCAMAYVAGPA
                     PAYFFGAAALAPAAFAFDVLDGRVARHRHTHSALGRELDSLSDVISFGVAPAALGFAA
                     GLRGGWDWIVLLYFVCCGVARLARYNVTAEALAGGDESGKVAYFEGTPIPTSVVLVAV
                     LALAAWRGRVGEQLWGGAWTLGPWDLHPLALLFALSGTLMISKTLRIPKL"
     gene            9084..10298
                     /locus_tag="VI319_04170"
     CDS             9084..10298
                     /locus_tag="VI319_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6863073.1"
                     /translation="MTPRATSVPATRRDAVATVALVVFCQGFQALTVGGIALFLPLIR
                     EDFGMTFSQAGALSAATTFTYMLGQIPAGYLTDRFGPKRLFFIGILGSTLLSLNFGTI
                     HSYPAAVANQLVSGVFRALLFAPGVTLIASWFPPDRKATAMGIFVIGGVSGNTFLSLV
                     GPLLVDRYGWRPPFIAFALIGVGAAFAYFFFGRERPAGPRHPVGMLDAFGLARYPIMW
                     VCAGIQFVRFGVTTSFHFWLPSLLVADRGLSLKTAGLITAMAFVLTGPSNAIGGYVSD
                     RLRNPPLVIGGSLAVLACTSALLVVVESVPAIVLVVAVNAVFIQFYFGPLFYVPVEVL
                     GQRVAGISTGFSNMFANIGGLTVAWALGAIKDAAGAFRWGYVATSALCLVGIGLTLAL
                     ARMRGAALQTKV"
     gene            10342..10695
                     /locus_tag="VI319_04175"
     CDS             10342..10695
                     /locus_tag="VI319_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863074.1"
                     /translation="MEDLLQNPPVLLMLIGCTLVILLTWTLDGIGRPGNAEAAEAPRR
                     PAPAVVRRLPAPAFDTPERCERPIGRYMDSTIFETVAIGGVEYRFDHVLGPGARWQSE
                     LGERCIAPGLVYLTR"
     gene            10719..11810
                     /gene="dinB"
                     /locus_tag="VI319_04180"
     CDS             10719..11810
                     /gene="dinB"
                     /locus_tag="VI319_04180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY6863075.1"
                     /translation="MVPRRIAHLDMDAFYASVELLRRPELREEPVAIGGDGDPMRRGV
                     VTTANYVARTYGIRSAMPLRTAYELCPRCVFLPVDFDEYRRVSRLFKAAIREVAPVVQ
                     DNGIDEAYIDLTELPGESGALARAIKDSVLRATALTCSIAVAPNKLLAKIGSELDKPD
                     GLTVLTDADLETRIWPLAVRRLNGVGPKSEAKLARLGIRTIGDLARQPLAGLIERFGA
                     SYGRWLHESAHGRDERPVVTFSEPKSRSRETTFERDTRDRGEVAGTLAELCAQVADDL
                     RSHGYAGRTIGVKLRTADFRTVTRAVTLAAATSDAGAIREAAFEALGRIPLERAVRLI
                     GVRVGNLCRAGGAPGVGDSPAAGVSLSLF"
     gene            11919..12734
                     /locus_tag="VI319_04185"
     CDS             11919..12734
                     /locus_tag="VI319_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863076.1"
                     /translation="MLAKIFAAAVAIGVSLPALAACPKHAFTDPADVHLTGGSLMIVT
                     HASSNDDGRIATKLGVDEAIRFAKGARMPVVYLQDDRPEEAYFMEDCSPDYWVSSTGE
                     VRFDVPASRVYLIGGHLEECLSATVHDVLLGWSRQPKRNLTLTYFMDGIFSNGRYVQQ
                     SDSWSGRYRRFISIVTYAKPAGEHFQKITLLETMGVIRDERKQYEYLERILPHFERTL
                     PDYRVEIKLMDAPARVLQRGRGERPPVLRFHFIDSAISVENAALFRTTESSAP"
     gene            complement(12763..12963)
                     /locus_tag="VI319_04190"
     CDS             complement(12763..12963)
                     /locus_tag="VI319_04190"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY6863077.1"
                     /translation="MAGVDVRFIDKGPIELKGSLELRDNDGRALQKKAGDAIYLCRCG
                     ASKSKPYCDGAHSAVGFDGKLA"
     gene            complement(13049..13453)
                     /locus_tag="VI319_04195"
     CDS             complement(13049..13453)
                     /locus_tag="VI319_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY6863078.1"
                     /translation="MNRSDPSTDPGRVRLDKWLWAARFFKTRSLAAQAVDGGRVKLNG
                     DRAKPSKDVKPGDRVEVAAGELRWDVEVLAVSARRGPAVEAAKLYAESEESRLRREQA
                     LARRRSAPHPAHDARGRPTKRDRRMIRRFTGS"
     gene            13530..14042
                     /locus_tag="VI319_04200"
     CDS             13530..14042
                     /locus_tag="VI319_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY6863079.1"
                     /translation="MSKIHAGAALAALAFAATGAQAQGLITTHKLSAALANELVGDAV
                     AACAQKGYKVVAVVVNLDGVRQAFLRGDGSPIHSVDNSYYKAYTVASLGLARNEQTTK
                     AIADRLAKNPPSNVPQTQLPNVTYAQGAVAIVAGSETIGALGVSGAPGGNFDEECARA
                     ALAKIKDRMK"
     gene            14153..14554
                     /locus_tag="VI319_04205"
     CDS             14153..14554
                     /locus_tag="VI319_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863080.1"
                     /translation="MTFIDTPTLDRLRDALLRLHKSLLDAQRIRYEREHGRVESRGEL
                     LELVLEDASFEWLRVLSSLIARLDELSAVDDKDVDEEVRAVIQRLLTLVRFEGNVGFT
                     APYREIIEAVPDALVAHVQLSRLLAELADPK"
     gene            complement(14758..15156)
                     /locus_tag="VI319_04210"
     CDS             complement(14758..15156)
                     /locus_tag="VI319_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863081.1"
                     /translation="MNKFAKFVTVLGAAAVFGCAATSQQNDSEYAKWQLLDEYEASMP
                     VLVGWGDYGSASRVALAIAIQRSQLEGTTPEVCQALSESRDLQRMASRSTEDLQVLVG
                     MQDSGAAMAGPRATCAHAQEVAARGQEGGF"
     gene            complement(15320..15724)
                     /locus_tag="VI319_04215"
     CDS             complement(15320..15724)
                     /locus_tag="VI319_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY6863082.1"
                     /translation="MKKLHRIADSKKIAGVCTGLGQYFDLDPVLFRLAFVVSVFFGGV
                     GILAYLVMWILVPEAPGQVSAPTSTKRLHLSSADRKIAGVCGGLGEWLDIDPVFARVA
                     FVLLALAGGFGVLLYVLLWLLAPRAPAAPVAG"
     gene            complement(15728..16972)
                     /locus_tag="VI319_04220"
     CDS             complement(15728..16972)
                     /locus_tag="VI319_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6863083.1"
                     /translation="MNKPERIPTTQAEEHVVGGVRMPRPFRIRRLGHFGVNVADPERS
                     KDFYCRLLGFRVADPIDFGERVPEDQKGRTGPTVGYFARHGTDHHSFVFFPKRTLESF
                     RPTARTPSGTVNQITWQTGSLQEVSDAFDWFTRRGKPIHRAGRDLPGSNWHFYPPDPD
                     GHTNELYYGIEQIGWDGYSKPRDMYGVRYAKPPQLPHKSEYAEVMQATQDGIPIEKGT
                     RAADLGREEKHDVGGILLARPFKIEKIGPVRLFAQDMDAMVSFYRDDLGLSLTEEVNY
                     KGHTCYFLRANTEHHTMALYPVALREELGLRSDTTLMSFGLRLGSYRQLRDAVAFLKQ
                     NGVTIKYLPPELFPGIDYCAFAIDPDGFAFQLHYYMEQVGWDGKPRPASQRPKIDNAK
                     WPETVEGRSDSFLGEVYMGPLG"
     gene            complement(17070..17807)
                     /locus_tag="VI319_04225"
     CDS             complement(17070..17807)
                     /locus_tag="VI319_04225"
                     /inference="COORDINATES: protein motif:HMM:NF015652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEY6863084.1"
                     /translation="MGEIHYQPLLVALSYVISVLGSYTALRLAVAIPQAQDRVSAIGW
                     TIGAATALGGGAIWSMHFIGMNAADMGMPVAYDATLTFASLVLAILAPGVGLYLVGRG
                     QGSVVKLVLAGIFTGLGVAVMHYTGMASMIMPATINYDPNLFKLSLVIAVAAATVAFW
                     LAFNLRGNLQRFGSALVMGVAVCGMHYTGMYALRLEHAAQASGSGVYLPPSAMGYTIF
                     AITAVLMLLLLVFNAWRVQQEMSEEIA"
     gene            complement(17851..18555)
                     /locus_tag="VI319_04230"
     CDS             complement(17851..18555)
                     /locus_tag="VI319_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863085.1"
                     /translation="MATVITISGADKTGALARLYSFFARKGYGVRGHQVSESDSGKLL
                     SISIDAARIDREALSAEIHELSPDYAVVGVVAEGTDTAATQKKTQPDAAALKEIAASF
                     PKIAGLVEKYAEGFDFGVRDLELFAAGKKLGSYHYKKEWSLGAPLKMPVALRRTLVPA
                     LEKMCQVEASDEEVVLKDSPFCGGDRLRCCCEFVTGFMQGFLDAGPGSAGSRVQKTGC
                     KAQGGPHAAYAIKYSA"
     gene            18794..19747
                     /locus_tag="VI319_04235"
     CDS             18794..19747
                     /locus_tag="VI319_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY6863086.1"
                     /translation="MANVLLVLTLPEPVRRQYVERLREAFPGVQVDMVDHHSKVDPYI
                     GDADVLITFGVQVAPHVFDKAKRLKWVQALGTGVDGIVDQPALGKDVMVTNMHGLHGG
                     PVSEAALAAMLALARDLPRSMRLQRAHRWERFPASLLKDKTVGIFGVGAIALELAPKC
                     KAFGMRVVGITSHRREAPGFDAMVGRDALERAVSELDFLVLLTPYSEATRNIVNASIF
                     AAMKPGACLVNLARGGIVDEAALVEALRAKKLAGAALDVFAEEPLPADSPLWDMDNVL
                     VTQHQGGFFDAYPRYALPVVEENMRRWLAGDLAGMVNVVRH"
     gene            19747..20952
                     /locus_tag="VI319_04240"
     CDS             19747..20952
                     /locus_tag="VI319_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY6863087.1"
                     /translation="MASERLNERLNCAISTRELERRWAAVRAAMTERRIDALVMQASN
                     DWMGGYVKWFTDVPATNGYPATVVFPRDERMTVIGQGPMGLERELPAEGDGVRRGVAR
                     FVGSPAYASCPYTAEYDARLAEKALAKYAGGTIGLVGRGAMSFAFGDYLQRKLSRARF
                     VDASDLVDWIKAVKSEEELELIRRTAAMQDACMEAAFRHIHAGMRDIEVAAIAEQIGH
                     KFGSEQGLFLAASAPVGVSAPFANRYQQNRVLREGDQFTLLIENNGPGGFYTELGRTC
                     VLGKASQEMKDEFAFVLEAQKFTLSLMRPGAKCSEIFAEYNAFMKRSKRPEERRLHAH
                     GQGYDMVERPLVRHDETMTIEKNMNMVCHPTYVTERTFSWCCDNWLIGASGPAEKLHR
                     FPQEIVELG"
     gene            20963..>21428
                     /locus_tag="VI319_04245"
     CDS             20963..>21428
                     /locus_tag="VI319_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6863088.1"
                     /translation="MNRKLLRPFIVTALLAAVEVAHAQSWPTRPVKVIVSQAAGGAPD
                     TICRLVTDRLGNALGQQFVVDNRPGSGNIIGAQAAARSAPDGYTLFFATAAALVTNPY
                     TFKSLPYDPVKDFAPVAMVAKGPFFVLAHPSVPAKTLSELVAYDKANPGKLAF"
BASE COUNT         3265 a         7545 c         7279 g         3339 t
ORIGIN      
        1 atgtcgggca gaccttggac gcgcgaggcg ccttgagcgg ggaggctgcc gtatccccga
       61 gcggccagtc tagcaaaagc aggggtttcc ggtctatcct gcgaatccaa tcgagaactt
      121 cggaccgctc gacaaggtct cttcggaacg gacgctcatg aatcgacgca tgctcctcca
      181 gaccctgacc gtggctttcc tgagcgccct gcaacgctcg ccggcggcgc agccggctca
      241 tggagatcga ggacgtcccg tgtcgataga cgacctgcag aaaaactgga aggcgttgct
      301 cgccgacggc gccgacgtgg cggcgagcgc ggagccgatc gagcgcgcga acgccgagtg
      361 gaggaagacg ctcacgccgg cgcagtacga cgtcctgcgc caggaaggca ccgagcgcgc
      421 gggcaccagc ccgctcgacc gcgagaaacg gcccggcgtg ttcgcctgcg cgggctgcgg
      481 gctgccgctg ttcacctcgg cgatgaagtt cgacagcggc accggctggc cgagcttctt
      541 cacgaccatc ccgggcatgc tcgcgaccaa gcgcgacttc aagctgatcg tgccgcgcac
      601 cgagtaccac tgcgtgcgct gcggcggcca ccagggccac gtcttcaacg acgggcccca
      661 gcccacggga ctgcgctact gcaacaacgg cgtggcgctg cgcttcatcc cgaaggcctg
      721 aaccggaagc tcgtctcggc ggcgcgcagc cgcgtctcgt cgcgcttcga caggccccag
      781 tggtcgatgc gtcccggcgg ccatttccag tcctcggggc caccgacctt gtagcccggg
      841 ccgacgcgct cgacctccga ggtgtattcg acgaccgcgc cgaacggcga cacgtaatag
      901 gcgaagacgt tgttgccggg accgtgccgt cccgggcccc acacgggatc gaggcccgcg
      961 tccttgagcc ggccgacgcc gcgcatgacg gcgtcgaggt cctgcatctc gaacgccatg
     1021 tgatgcagcg cgggcgcgcc cgtgcgcacg tacgcgagcg agtggtgcgt gctgtcgcag
     1081 cggatgaagt ccatcatctt cgtgcgatcc gagagcttga accccagcac ctcgagcgcg
     1141 aacttcgtgc acgcgtcggc gtcggccgcg ctgatcggca cgtgcgtgag gccgatcggg
     1201 cggtcggcgt ccttgatccg ggtcgcgggc ttcttgtcgc tgacgaagcg gaacacctgc
     1261 ccttccggcc ccgcgagcgt gaagcccttc ggcgcgccgg gctcgccggg ccgaggcgcg
     1321 tagggcgtga tcgcgcggcc ggccttcttc gcgcgggcct tcacgcgctc gagctccttc
     1381 ggcgcgccgg caaaggtgac cgacacgagc gccgggcccg gcgcctcggc gatcgccatc
     1441 acatagggat gctcgcccgt gccgcggagg tacacggttc cgccgctctt ccccgcctcg
     1501 atcaggcccc atgggccggt gaggaattga acggccgcgt cggcgcgcgg caactcgagc
     1561 tccacgctgc gcaatttcat ggagaagact ccttcggttc gctcgccgga atcatagcgc
     1621 agcgccccgt acaatgaggg atgtccgacg acaacgcccc ggcctacgcg ggcctcaccc
     1681 ccgagcgcgt gctcgacgcc ctcgacgccg tcggcttccg cggcgacggg cggctgctcg
     1741 ccctgaacag ctacgagaac cgcgtctacc aggtgtggct ggaggaaggc ggctcggtgg
     1801 tcgcgaagtt ctaccggccg gagcgctgga gcgacgcgca gatccacgag gagcacgcgt
     1861 tcgcgagcga gctcgccgat cgcgagatcc cggccgtccc gccgatcgcc gacgcgcgcg
     1921 gcgcgacgct gcacgcgcac gaaggcttcc gcttcgccgt gttcccgcgc aagggcgggc
     1981 gggcgcccga gctcgaggat cccgccgtgc tcgagtggct cgggcgcttc ctcggccgca
     2041 tccacgcggt cggcgcgaca cggcctttcg cgcaccgccc ggcactcgat ctcgagacct
     2101 tcggccgcga gccgcgcgac tggctgctcg ggcacggctt cgtgccggcg gacctgctcg
     2161 aggcgtggcg agccgccgtc ggccaggccc tcgacggcgt cgcgctgtgc tacgagcgcg
     2221 ccggcggcgt gaagacgctg cgcacgcacg gcgactgcca ttccggaaac gtgctgtgga
     2281 ccgacgaagg cccgcatttc gtcgacttcg acgacgcgcg caccgcgccc gccgtccagg
     2341 acctgtggat gctgctctcg ggcgaccgcc ccgcgatgac gcgccagctc ggcgacctgc
     2401 tcgcgggcta cgaggacttc atggacttcg accggcgcga gctgcatctg gtcgaggcgc
     2461 tgcgcacgct gcgtctcgtg cactactcgg cgtggatcgc gcggcgctgg agcgatcccg
     2521 cgttcccggc ggccttcccc tggttcggca cgcagcgcta ctggcaggac cgcatcctgg
     2581 agctgaagga gcagatcgcg ctcatggccg aggagccttt gcacgcgtga gctacaatcg
     2641 cccccttcca tcgcagccca cccgggagcc gacatgccgg tcgcaaaagt ccgaggcctg
     2701 aacatccagt accgggtcgt cggcgagcgc gggccgtggg tcacgctcac caccggcggc
     2761 cggcgcggcc accaggagtt cgtagcgctc gcgcagaaga tcgccgacgc cggcttccgc
     2821 gtgctgctgc acgaccggcg caacaccggc gcctccgaca tcctgatcga aggcgacgag
     2881 accgaggagg cgatctgggc cgacgacctc tacgagctgc tgcggcagct cggcgccgcg
     2941 ccggccttca tcggcggctc gtcgtccggc gcgcgcacct cgatcctgtt cgcgcttcgc
     3001 caccccgacg cagcgcgcgg cctgttgctc ctgcgcgtga cgggcggcgc cttcgcggcg
     3061 aagcgcctgc ccgagatgta ctacgaccag ttcctgcgcg cggcgaagga aggcggcatg
     3121 gccgccgtgt gcgcgaccga gcagtaccgc gagcgcttcg ccgagaaccc ggcgaacctg
     3181 gagcgcatga agaagaccga cgtgaagcag tacgtcgccg tcatgacgcg ctggcgcgac
     3241 aacttcgtcg ccggggcgag cctgcccgtg atgggcgtga cggaaaagga gctgcgctcg
     3301 atccgggtgc cgacgatcgt catcccgggc aacgaccgga cgcattcgtc cgagagcgcg
     3361 cgcgtcgcgc accgcatgat cccggggagc gagctgcacg cgctgcccat cacggaccag
     3421 gacgtcgacc tgatcccgtt cccgaagtgg gcgccctacg aggacgagat cgcgcgcgtc
     3481 ttcgtcgatt tcatgaggcg caacggcgcg cgctgagcgc cgggctcagg cgacggtctc
     3541 gggcaccttc cccaggcgcc cggcctggaa gtcggcgaag gcctgctgga tctcctcgac
     3601 gctgttcatg acgaacggcc catagcccac gatcggctcg tcgatcggct cgccgttcat
     3661 cacgaagacc gtcgcatcgg gcgcgccctc gatctccacc ttgccgttct cgcgtccgag
     3721 aacgacgaga tcgccggcgc gcgctccgcg ctcgccgttc acgatcacct cgcccgcgac
     3781 cacgaacagc gccgcggtcc agccctcgcg cagctcgagg cgcacgcggt ggtcgccctt
     3841 cagtcgcacg tcgagcaggt tgatcggcgt gaaggtgcgc gccgggccct tcgcgccctg
     3901 gtactcgccc gcgatcacgc gcaccgagcc gccgtcgcgc ggcagcttca cgaccgggat
     3961 gtcggcggcg agcagcgtct gatagcgcgg ctcggtcatc ttcgaggcgg ccggcaggtt
     4021 gacccagagc tggagcatct cgaagcgtcc ccccgagcgc gcgaaattgc gcccgtgcat
     4081 ctccttgtgc agcacgcccg cgcccgcggt catccactgc acgtcgccgg ggccgatcgt
     4141 gccgcggctg ccggtgctgt cctcgtggtc gacctcgccc tcgtaggcga cgctgaccgt
     4201 ctcgaagccg cggtgcgggt gccagcccac gccgcgctcg cgcgcggtcg gctcgaaatc
     4261 ggcgggaccc gcatggtcga gcaggaggaa aggactcagc tcggacgcga ggtcctggta
     4321 gctgaacacc gtgtgcacgg ggaagccgtc gcccacccag tgaggctcgc tgccgcggta
     4381 gatgcctttg atcgctttca tggggtcctt tccgtccaga ccgggtgcag atgggggcat
     4441 tttggcattg cacaagccgg ttccggaaac gtgtcgggct gcggcgacca cgataggccg
     4501 gggacaaaat cgccgcgcgg cggcgtgaat tagtgcacgc gacccgcggc gctggcacgc
     4561 aactggctat gacagggaca tacgcatttc gcatatgacg gagacattcc cgtgagccag
     4621 aagccgaata gcctcgagcc cagccggccc gccggccggc gccgcgtcgt cgaccgggta
     4681 cgcgagcgag cgctgctgcg cgccatgcgt ggggccctgc gccgcagcgt cgtccttctc
     4741 cccctgaccg aggaagacga tctgctccag cgattcctgg tcgacgcgct cggcctcgtc
     4801 cccgcctagg aataaaaaag ccgcgctgct cggcgcggct ttccgggtga cagtttcggg
     4861 ggcttcagcc gaacaggctc tcacgctccc gatcgatcag ctcgtcgcgg cccttcaggg
     4921 tgatcgtata ggtctggccg ttcggcaccg cataaccgcg aaggcgcagc ttcatcgcta
     4981 cgtgcagggg aatgcttgag tgttgcgcat cgcgcattgc gcgcagccaa gtgacgtcct
     5041 cgacgagcag cgtccgatcc atgacacctc cttgaaagca ccgactcccc tgacgctccc
     5101 tgattgccga atagggcctc cttattgttg ttgcgaccct ggtccggatg gagcaacggc
     5161 tgtgccactc tgaaatattg ataggccaca gggacttagg aaaagcggac tgtttccgaa
     5221 ggggaaagag tcgtctttcg cgggcgacgc gagcgaccac tatctatagc acctatctga
     5281 ttacaaacga taaacgatgt cgcggtcagg cgacaggatc agcgaccgaa acggtcgcag
     5341 acttcgggcg gtccctgcca gttctgctcg gcaacgtgcc cggcttcgaa gatgagcgtg
     5401 cgctcgcatt ggacctgctc ggacccgccg gagccgaaga tcaccccggt cccgatcccg
     5461 aatccgccgg agccgacccc gatcccgacc gagggcgaca cccccgtcct ggggcgcgaa
     5521 ccctcccagg cccaggtata ggcctgccga ccgtcggaca gagtggcgct tcggaccgga
     5581 gtgccccagg cgcggactac ctcgtcgtag gtggtcccga gccacgcctg gcgggggtcc
     5641 ggggcgggct cgcgcgaggc gcagccggcc acggccagcg ccgccacgag gcaaagaccg
     5701 aggatgcgcg cgcccatgat ctctcggatg cccgcgccgg gcgtccgttc acgacatcgg
     5761 ctcgggcggg ggagcgtccg ccggcagggg tatgaactcc cggtcgtcgc gctcgggaag
     5821 cttcatccgg ccggcgcgcc agtcggcctt ggcgcgctcg atgcgctccc ggctcgaggc
     5881 cacgaagttc cactcgatgt agcgctcgcc gaccggctcg ccgccgagga gcatcaagac
     5941 tgacggcgcg cgcgcctcga acaccaccgg ctgtcccggc gcgagcacga gcatgcggcc
     6001 cgccgaatgg cgctcgcccg cgacttcgac ctcgccttgc acgatgtagg ccgcgcgctc
     6061 gggatactgg gccgggagct gtgccttcgc gcccgcggcg agctcccagt gcacgtagaa
     6121 catcggcgaa tgcgtgcgca ccgcggcgcg cgccccgaac gcctcgccgg cgagcagccg
     6181 cgcggccagg ccgccgcctt cgtagaacgg caggtcgccc ggaccgtgat gggcgaacgc
     6241 gggctcggac tcctcggcct cgcgcggcag cgcgacccag gcctggatgc cgtgaaggcg
     6301 cccgccctcg gcgcgcgcgc gctcgaagcg ctccgaatgc gtgatgccgc gccccgccgt
     6361 catccagttc acctcgccgg ggcggatcgc ctgctccgag ccgacactgt cccggtgcat
     6421 gatctcgccc tcgaagagat aggtcaccgt cgagagcccg atgtgcggat gcggtcgcac
     6481 gtcgaccgtg cgcggcacgc cgcgcggcag ctccacgggg cccatgtggt cgaagaagat
     6541 gaacggcccc accatccggc gcttcgcgtg cggcagaacg cgcgcgacct cgaagccgcc
     6601 gagatcgcgg cgccgcgcct cgatgacgag ctcgatcatc gcttggtttc gtcgcctgga
     6661 tttccccggt tccacgatag caaataaact gccggcatga gcgagctcga caccctccgg
     6721 acggacgtcg tcggcagcct gctgcggccg gccgactggc gcgaggcgcg cacgcgcttc
     6781 gagcgcggcg agatcgacgc gggcgcgttc gcggcgatcg agcgcgaatg cgtgcgccgc
     6841 gcgatcgcgc tgcaggagag cgcgggactc ggcgtggtga ccgacggcga gctctcgcgg
     6901 ctcaatttcc aggacagctt cggcatggcg gtttcgggat acgacgcggc gcccgaccgg
     6961 ctgcacggct acgagcggcg caccgacggc gcggtgccgc tgaagcgctg ggacatgccc
     7021 gacgtggccg agcgcggcac gccggtgtcg caccggcggc cggtggtcgc gcgcctcgcg
     7081 ctcgtgcgca acgtgccgct cgaggagtac gagcgcgcgc ggccgctcgc gaagcggccg
     7141 ctgaaggtca gcctgatcgg gcccgatcgc gtgctgcagc gcttcgacca cgcgggctcg
     7201 aagcgggttt acgcgagcgt ggacgacttc ggcgccgacg tggtcgcggt ccagcgccgg
     7261 atgatcggcg gcctcgtcga cgccggatgc cgctatgtgc acatcgacgc gcccggcctg
     7321 accgcctacg tcgacgagcc ttcgctcgcc gcgatgcgcg cgcggggcga agacccggcg
     7381 cagaacctgg cgcgcgggct agccgccgag gccgcggtcg tgcgcggctt cgacggagtg
     7441 accttcggcc tgcacctgtg ccgcggcaac cagcgcagca tgtggcatcg cgaaggcgcg
     7501 tacgacgcga tcgccgagca gatgttcggc acgctgccgc accggcgctt cctcctcgaa
     7561 tacgacacgc cgcgcgccgg gagcttcgcg ccgctgcgtt tcgtgccgaa ggaccgcatc
     7621 atcgtgctcg gtctggtcag caccaaggtg ccacggctcg agaccgtgga cgagctgcgc
     7681 cggcgcatcg acgaggcggc gaaggtcgtg ccgctcgagc gcctcgcgat cagcccgcag
     7741 tgcgggttcg gctcggatgt cgccggcaac ctcgtcggcg aggacgacca gaagcgcaag
     7801 ctcgagcgcg tcgccgagac ggcgcggctc gtgtggggct gagccctagt acgcgaagct
     7861 gagtccgacc tcgggccagg tatcgctgca gtggcggtac gtgccttcgt cgcaacccga
     7921 gatgcgctcg tggacgatgc cgacgccgag cgacgcgccg cggttgatcg tgaacgcgaa
     7981 gcccagccgc gccccgaacg tgttcttgtc gggcagcccg tcatagaagg tgcgcgacac
     8041 gaacagcgcg ccgtacggcc ttgccctcgg gttcgtcggc acggtgtagc ggatctccgg
     8101 gacgagcttg tagatcgaag gatccttgcc gcgccaggcc tcgagcccga ggctgccctc
     8161 gaagtcgtcc gcgaagtagt ggccgacgcg gcccgacagg accgtgtagg tctggtcgag
     8221 ctggcgtccc ggcgcgagcg agaggccgaa gctcgaggag ccctggtcgg ccgcgcgcgc
     8281 accgggcgcg gcgagcagca cggcaacggc gatcaggcgg agcaggcggc ggttgtgcgg
     8341 catggagatc tcctcgaagg aacgccgcaa gataccagaa atcggccgcg gcccgcgtcg
     8401 atgatcttcg gcgtgcgcgc agagcgaagc cgcgagtacg ctacactccg cggcatgccc
     8461 gccgagccgc cgaagcattt ctcgatgatc cgcggcctgc acctggccga tgcgttcacg
     8521 ctcgcgaatg ccgcgtgcgg cgtcgcggcc gtgttctgcg ccatggccta cgtcgcgggt
     8581 cccgcgcccg cgtatttctt cggcgccgcg gcgctcgcgc cggccgcgtt cgccttcgac
     8641 gtgctcgacg ggcgcgtggc gcggcatcgc cacacgcact cggcgctggg gcgcgagctc
     8701 gattcgctct cggacgtcat ctcgttcggc gtcgcgccgg ccgcgctcgg cttcgcggcc
     8761 ggcctgcgcg gcggctggga ctggatcgtg ctgctctatt tcgtctgctg cggcgtggcg
     8821 cggcttgcgc gctacaacgt caccgccgag gcgctcgcgg gaggcgatga gagcggcaag
     8881 gtcgcctact tcgaaggcac gccgatcccg accagcgtcg tcctcgtcgc ggtgctcgcg
     8941 ctcgccgcgt ggcggggccg cgtcggcgag cagctgtggg gcggcgcctg gaccctcggt
     9001 ccctgggacc tgcacccgct cgcgctgctg ttcgcgctct ccgggacgct gatgatcagc
     9061 aagacgctgc gcatcccgaa gctgtgacgc cgcgggccac ctcggtcccg gcgacgcggc
     9121 gcgacgccgt cgcgacggtc gcgctggtcg tcttctgcca gggcttccag gcgctcacgg
     9181 tcggcggcat cgcgctgttc ctgccgctga tccgcgagga cttcgggatg acgttctcgc
     9241 aggcgggagc gctgtcggcg gcgaccacgt tcacctacat gctgggccag atccccgcgg
     9301 gctatctcac cgaccgcttc ggcccgaagc ggctgttctt catcggcatc ctcggctcga
     9361 cgctgctctc gctcaacttc ggcacgatcc actcgtatcc ggcggcggtc gcgaaccagc
     9421 tcgtgtcggg cgtgttccgc gcgctgctgt tcgcgccggg cgtgacgctg atcgcgtcgt
     9481 ggttcccgcc cgaccgcaag gcgaccgcga tgggaatctt cgtgatcggc ggcgtgtcgg
     9541 gaaacacctt tctctcgctc gtcggccccc tgctcgtcga ccgctacggc tggcgcccgc
     9601 ccttcatcgc gtttgcgctg atcggcgtcg gcgccgcatt cgcttatttc ttcttcggcc
     9661 gggagcggcc cgccggcccc aggcacccgg tcggcatgct ggacgcgttc ggcctcgcgc
     9721 gctatccgat catgtgggtg tgcgcgggca tccagttcgt ccgcttcggc gtgacgacct
     9781 cgttccactt ctggctgcct tccctgctcg tcgccgaccg cggcctgtcg ctgaagacgg
     9841 ccggcctgat caccgcgatg gcgttcgtcc tcaccgggcc gtcgaacgcc atcggcggct
     9901 acgtctccga ccgcctgcgc aacccgccgc tcgtgatcgg cggctcgctc gccgtgctcg
     9961 cctgcacgtc cgcgctgctg gtggtcgtcg aatcggtgcc cgcgatcgtg ctcgtggtcg
    10021 cggtgaacgc ggtcttcatc cagttctact tcggcccgct gttctacgtc ccggtggagg
    10081 tcctcggcca gcgcgtcgcg ggcatctcga cgggcttcag caacatgttc gccaacatcg
    10141 gcggcctgac ggtcgcctgg gcgctcggag cgatcaagga cgcggccggg gcgttccgct
    10201 ggggatacgt cgcgaccagc gcgctgtgcc tggtcggcat cgggctcacg ctggcgctcg
    10261 cgcgcatgcg cggggccgcg ctgcagacga aagtttgatc cggatcaaag cgcgggcggc
    10321 ggggaaggcc attctggatg catggaagac ctgctccaga acccgcccgt gctgctcatg
    10381 ctgatcggct gcacgctcgt gatcctcctc acgtggaccc tggacggcat cggccggccg
    10441 ggaaacgcgg aggcggcgga agccccgcgc cgcccggcgc ccgcggtcgt gcgcaggctg
    10501 ccggcgccgg ctttcgacac gcccgagcgc tgcgagcgtc cgatcggccg ctacatggac
    10561 tcgaccatct tcgagacggt cgccatcggg ggcgtcgaat accgcttcga tcacgtgctc
    10621 gggcccggcg cgcgctggca gtccgagctc ggcgagcgct gcatcgcccc gggcctggtg
    10681 tacctgacgc gctagaattc cgggcacgga gcccggaatt ggtcccccgc cgcatcgcgc
    10741 atctcgacat ggacgcgttc tacgcgtcgg tggagctgct gcgccggccc gagctgcgcg
    10801 aggagccggt cgcgatcggc ggagacggcg atcccatgcg ccgcggcgtg gtcaccaccg
    10861 cgaactacgt ggcgcgcact tacggcatcc gctcggcgat gccgctgcgc accgcgtacg
    10921 agctgtgccc gcggtgcgtg ttcctgccgg tcgacttcga cgagtaccgc cgcgtctcgc
    10981 gcctgttcaa ggcggcgatc cgcgaggtcg cgccggtcgt gcaggacaac ggcatcgacg
    11041 aggcctacat cgacctcacc gagctcccgg gcgagtccgg cgcgctcgcg cgcgcgatca
    11101 aggacagcgt gctgcgcgcg accgcgctca cctgctcgat cgcggtcgca cccaacaagc
    11161 tgctcgccaa gatcggctcg gagctcgaca agcccgacgg gctcaccgtg ctcaccgacg
    11221 ccgacctcga gacgcgcatc tggccgctcg ccgtgcgccg actgaacggc gtcggcccca
    11281 agagcgaggc gaagctcgcg cggctcggca tccgcaccat cggcgacctc gcgcggcagc
    11341 cgctcgcggg cctgatcgag cgcttcggcg cgagctacgg gcgctggctg cacgagagcg
    11401 cgcacggccg cgacgagcgc ccggtcgtga ccttcagcga gccgaagtcg cgcagccgtg
    11461 agaccacctt cgagcgcgac acgcgcgacc gcggcgaggt cgccgggacg ctggccgagc
    11521 tgtgcgccca ggtggccgac gacctgcgct cgcacggata cgcgggccgc accatcggcg
    11581 tgaagctgcg caccgccgat ttcaggacgg tgacgcgcgc cgtgacgctc gccgccgcga
    11641 cgagcgacgc cggcgccatc cgcgaggcgg ccttcgaggc gctcggccgc ataccgctcg
    11701 agcgcgcggt gcgcctgatc ggcgtgcgcg tgggcaatct gtgccgcgcg ggaggcgcgc
    11761 ccggcgtcgg ggattccccg gcagccggcg tcagcctcag tctcttttag gctctctttt
    11821 aggctctctt ctaggccctc ttctgacatc tcgctgacag cccgcgccgc gcgtgcgggc
    11881 ttgataaaat ttcggctatg gcgttaggga gagccaacat gctcgcgaaa atcttcgctg
    11941 cagcggtcgc catcggggtg tcgctgcccg cgctcgcggc gtgcccgaag cacgcgttca
    12001 cggaccccgc cgatgtgcac ctgacgggcg gctcgctgat gatcgtgacg cacgcgtcct
    12061 cgaacgacga cgggcgcatc gcgaccaagc tcggcgtcga cgaggcgatc cgctttgcca
    12121 agggagcgcg catgccggtc gtgtacctgc aggacgaccg ccccgaggaa gcgtatttca
    12181 tggaggactg cagcccggac tactgggtgt cctccaccgg cgaggtccgc ttcgacgtgc
    12241 ccgcgtcgcg cgtctacctg atcggcgggc acctcgagga atgcctctcg gcgaccgttc
    12301 acgacgtgtt gctcgggtgg tcgcgccagc cgaagcgcaa cctcacgctg acctacttca
    12361 tggacggcat cttctcgaac ggccgctacg tgcagcagag cgactcctgg agcggccgct
    12421 atcgccgctt catcagcatc gtcacctacg ccaagcccgc gggcgagcat ttccagaaga
    12481 tcacgctgct cgagaccatg ggcgtgattc gcgacgagcg caagcagtac gagtacctcg
    12541 agcgcatcct gccgcatttc gagcgcaccc ttcccgatta ccgggtcgag atcaagctga
    12601 tggacgcccc ggcgcgggtg ctgcagcgcg gacgcggaga gcgtccgccg gtcctgcgct
    12661 tccacttcat agactccgcg atcagcgtcg agaacgcggc gctcttccgg acgacggaaa
    12721 gctcggcgcc gtagcggcgc ccttgcgtat cattcggccc ctttaggcga gcttgccgtc
    12781 gaacccgacg gccgagtgcg cgccgtcgca atagggcttc gacttcgacg cgccgcagcg
    12841 gcacaggtag atcgcgtcgc cggccttctt ctgcagcgcc cgaccgtcgt tgtcgcgcag
    12901 ctccaggctg cccttcagct cgatcggacc cttgtcgatg aatcggacat ccactcccgc
    12961 catttgccgt tctccggaaa agttggaaag cagcacgata ggatgcccgg ccgtccggtt
    13021 cttgatctgg atcagacccg cgccggcttc agctgccggt gaagcggcgg atcatgcgcc
    13081 gatcgcgctt cgtcgggcgg ccgcgcgcat cgtgcgccgg atgcggcgcg gaccggcgcc
    13141 gcgccagcgc ctgctcgcgc cgcagccggc tttcctcgct ctcggcgtag agcttcgccg
    13201 cctcgaccgc cggtccgcgc cgggcggaga cggccagcac ttcgacgtcc cagcgcagct
    13261 ccccggcggc gacctcgacg cggtcgccgg gcttcacgtc cttcgacggc ttggcgcgat
    13321 ctccgttcag cttcacccgg ccgccgtcga cagcctgcgc cgccaggctg cgggtcttga
    13381 agaagcgggc ggcccacagc catttgtcga ggcgcacgcg gccggggtct gtggacggat
    13441 cggatcgatt catgcgcttc ggcgactcgg gcggagctcg ggattttgac gtatcgttgc
    13501 ggtcccgcca aacccccagg aggggaccca tgtcgaagat ccacgcagga gccgcgctcg
    13561 cggcgctcgc cttcgccgca acgggcgcgc aggcgcaggg gctcatcacg acgcacaagc
    13621 tttccgcggc gctcgccaac gagctggtcg gcgacgccgt ggccgcatgc gcgcagaagg
    13681 gctacaaggt ggtcgcggtg gtcgtgaatc tcgacggcgt gcgccaggcg ttccttcgcg
    13741 gtgacggctc gccgatccac tcggtcgaca actcgtacta caaggcgtac accgtcgcgt
    13801 cgctgggcct cgcccggaac gagcagacga ccaaggcgat cgccgaccga ctggcgaaga
    13861 acccgccgag caacgtcccc cagactcagc tgcccaacgt gacctacgcg cagggcgctg
    13921 tcgcgatcgt cgccggcagc gagacgatcg gcgcgctcgg cgtgagcggc gctcccggcg
    13981 gcaacttcga cgaggagtgc gcgcgcgcgg cgctcgcgaa gatcaaggac cggatgaagt
    14041 agcgccaaat tcgagtcggc gtcgcaaaac gccccggtcc ccggggcgtt ttgtttttga
    14101 aacggccgcc ccaatgtacg gtcttaccgg attgccggcc aaaagcgacg ccatgacttt
    14161 catcgacacg ccgaccctcg accgccttcg cgacgccctg ctgcggctgc acaagtcgct
    14221 gctcgacgcg cagcgcatcc gctacgagcg cgagcacggg cgggtggaat cacgcggcga
    14281 gctgctcgag ctcgttctcg aggatgcgtc gttcgagtgg ctgcgcgtgc tgtcctcgct
    14341 catcgcgcgg ctcgacgagc tctcggccgt ggacgacaag gacgtcgacg aggaagtgcg
    14401 agcggtgatc cagcgcctgc tgacgctggt gcgcttcgag ggcaacgtcg ggttcaccgc
    14461 gccttaccgt gagatcatcg aggcggttcc ggacgcgctc gtggcgcacg tgcagctctc
    14521 gcggctgctc gcggagctgg cggacccgaa atgaaaacgc cccggagatc gctctccggg
    14581 gcgtctgtcc tgctaaggca cgccggccta gcggctggcg gagcccccgg tactgcggat
    14641 ggatccgcgg tgcgaggccg ccaaaccggt cctgccttgt gggtctctca cgggatcacc
    14701 tcctttcaaa gttgtcagcc gccagtttag caacgcggct cgactccgaa aagagggtca
    14761 gaagccgcct tcctggccgc gcgcggccac ttcctgggca tgcgcgcacg tggcgcgcgg
    14821 gcccgccatc gcggcaccgc tatcctgcat cccgacgagc acctggaggt cctcggtgct
    14881 gcgcgacgcc atgcgctgaa gatcgcgcga ctccgacagc gcctggcaga cttcgggcgt
    14941 cgtgccttcc agctggctgc gctggatcgc gatcgcgagc gcgacacggc tggcggagcc
    15001 gtagtcaccc cagcccacga gcaccggcat cgaagcctcg tattcgtcga gcagctgcca
    15061 cttcgcgtac tcggaatcgt tctgctgcga cgtcgcggcg cagccgaaca ccgcggcagc
    15121 gcccagtacc gtgacaaact tggcaaactt gttcattgct cccccgctcc tttgtttgaa
    15181 accgcgacac ccctgaccgg gaattgccgc tccgggcccg atttcctgtg ctaacccggg
    15241 cttgggatca atcgagcaag gcaggtgcca gcgccgcggg cgcggcgtga aaaatccctc
    15301 ggatcgaggg atgtgggggt caaccggcga ccggagcggc cggagcgcgg ggcgcgagca
    15361 gccagagcag gacgtagagc agcacgccga agcctcccgc caacgcgagc agcacgaagg
    15421 cgacgcgcgc gaaaacggga tcgatgtcga gccactcgcc caggccgccg cacacgccgg
    15481 cgatcttgcg gtcggcgctc gagaggtgca ggcgcttggt agacgtcggc gcagaaacct
    15541 gccccggcgc ttcggggacg aggatccaca tcaccaggta cgcgagtatc ccgactccgc
    15601 cgaagaaaac cgacaccacg aaggccagtc ggaacagcac cggatccagg tcgaagtact
    15661 ggcccaggcc ggtgcagacg ccggcgatct tcttcgagtc tgcgatgcgg tggagcttct
    15721 tcatcgacta tccgagcggc cccatgtaga cctcgccgag gaaactgtcg cttctgcctt
    15781 ccacggtctc gggccacttg gcgttgtcga tcttcggccg ctgcgacgcg gggcgcggct
    15841 tgccgtccca gccgacctgc tccatgtagt agtggagctg gaaggcgaag ccgtccgggt
    15901 cgatggcgaa agcgcagtag tcgatgccgg ggaagagctc cggcggcagg tactttatcg
    15961 tcaccccgtt ctgcttcagg aatgcgaccg cgtcgcgcag ctgccggtag ctgccgagtc
    16021 ggagcccgaa agacatcagc gttgtatccg atctcaggcc gagctcctcg cgcagcgcca
    16081 ccgggtacag cgccatcgtg tggtgctcgg tgttcgcgcg caggaagtag cacgtgtggc
    16141 ccttgtagtt cacctcttcg gtgagcgaca gcccgaggtc gtcgcggtag aacgagacca
    16201 tcgcgtccat gtcctgggcg aacagccgca ccggcccgat cttctcgatc ttgaacggcc
    16261 gggcgaggag gatcccgccg acgtcgtgct tctcctcgcg cccgagatcc gcggcgcgcg
    16321 ttcccttttc gatcggaatg ccgtcctgcg tcgcctgcat gacctcggcg tactcggact
    16381 tgtgcggcag ctgcggcggc ttggcgtagc gcacgccgta catgtcgcgc ggcttcgagt
    16441 agccgtccca gccgatctgc tcgatgccgt agtacagctc gttggtgtgg ccgtccgggt
    16501 ccggcgggta gaagtgccag ttggatccgg ggagatcgcg gccggcgcgg tggatcggct
    16561 tgccgcgccg cgtaaaccag tcgaacgcgt ccgagacctc ctgcagcgaa ccggtctgcc
    16621 acgtgatctg gttcaccgtg ccgctcggcg tgcgcgcggt cggacggaac gactcgagcg
    16681 tgcgcttcgg gaaaaacacg aacgagtgat gatccgtgcc gtgccgagcg aaatagccga
    16741 ccgtcggccc ggtcctgccc ttctggtcct cgggcacgcg ctcgccgaag tcgatcgggt
    16801 cggccacccg gaagccgagc agccgacaat agaagtcctt gctgcgctcg ggatcggcga
    16861 cattgacgcc gaagtgcccg aggcggcgaa tgcggaacgg ccggggcatc cgcacgccgc
    16921 cgacgacgtg ctcttccgcc tgcgtggtgg ggatccgttc gggcttgttc atcgggcctc
    16981 ctcgcgccgc ggctgcggca ggttcgcccg attttacgcc caaaaaaaag cccggctcga
    17041 ggccgggctt tctccggtgc gccggagact tacgcgatct cttccgacat ctcctgctgc
    17101 acgcgccagg cgttgaacac gagcagcagc agcatcagca ccgccgtgat ggcgaagatc
    17161 gtgtacccca tcgccgaggg cggcaggtac acgcccgacc cgctggcctg ggccgcgtgc
    17221 tcgaggcgca gggcgtacat gccggtgtag tgcatgccgc acacggcgac gcccatcacc
    17281 agcgcgctcc cgaagcgctg caggttgccg cgcaggttga acgccagcca gaacgccacc
    17341 gtcgcggccg cgacggcgat caccagggac agcttgaaca ggttcgggtc gtagttgatc
    17401 gtcgccggca tgatcatcga cgccatgccc gtgtagtgca tgaccgcgac gccgagaccg
    17461 gtgaagatcc ccgcgagcac cagtttgacg acgctgccct gcccgcggcc gaccaagtaa
    17521 agaccgacgc cgggcgccag gatcgccagc accagcgacg cgaacgtcag cgtggcgtcg
    17581 tacgccaccg gcatgcccat gtcggctgcg ttcatgccga tgaagtgcat cgaccagatc
    17641 gcgccgccgc ccagcgcggt cgccgcgccg atcgtccagc cgatggccga cacccggtcc
    17701 tgcgcctgcg ggatcgcgac tgcgagccgc agagcggtgt aggacccgag caccgagatc
    17761 acgtacgaca gcgccacgag caacggttga taatgaattt cgcccatcgt gcttcctccc
    17821 ttctggattg atccgaaaac cgcctcgccg tcacgcggag tacttgatcg cgtacgcggc
    17881 gtgcggacct ccctgcgcct tgcaacccgt cttctgcacg cggctgccgg cggagccggg
    17941 acccgcgtcc aggaaaccct gcatgaaacc ggtgacgaac tcgcagcagc accgcagccg
    18001 gtcgccgccg cagaaggggc tatccttcag caccacctcc tcgtcgctcg cctcgacctg
    18061 gcacatcttc tcgagagccg gcacgagcgt gcgccgcagc gccaccggca tcttcagcgg
    18121 cgcgcccagc gaccattcct tcttgtagtg gtacgaaccg agcttcttgc ccgctgcgaa
    18181 caactccagg tcgcgaacgc cgaaatcgaa gccctcggcg tacttctcca cgagcccggc
    18241 gatcttcggg aagctcgccg cgatctcctt gagcgcggcc gcgtcgggct gcgttttctt
    18301 ctgggtcgcg gcggtatcgg tcccttcggc cacgacgccg accacggcat aatccggact
    18361 gagctcgtgg atctcggccg acagcgcctc gcggtcgatg cgcgcggcgt cgatgctgat
    18421 gctcagcagc ttgccgctgt ccgactcgct cacttggtgc ccgcgtacgc cgtagccctt
    18481 gcgcgcgaaa aagctgtaaa gccgcgccag agcgccggtc ttgtccgcgc cgctgatcgt
    18541 gatcacggtg gccatgactg cctcccctac gtaagtgtca gttcctgccg gacagtcagc
    18601 aagttgcgtg ccggtcgaat atgaccccgc catagggcgt aacgggcgtt ttgatgtgca
    18661 atttgccctg cgatccgggt ttccccggtg tcagcgcccc gtcatgcgtg cgcccgcggc
    18721 cccgccttga caaggcggga agccggattt gaaaatgaac gtgttcggga atttttcccg
    18781 ccggccccac tcgatggcga acgtcctgct cgtcctgacc ctcccggagc cggtgcgccg
    18841 gcagtacgtc gagcgtctgc gcgaggcctt tcccggggtc caggtcgaca tggtcgacca
    18901 ccacagcaag gtcgatccct acatcggcga cgccgacgtg ctcatcacct tcggcgtgca
    18961 ggtggcgccg catgtcttcg acaaggcgaa gcgcctgaag tgggtgcagg cgctcggcac
    19021 gggcgtcgac ggcatcgtcg accagccggc gctcgggaag gacgtcatgg tcaccaacat
    19081 gcacggcctg cacggcggcc cggtgtcgga ggccgcgctc gcggcgatgc tcgcgctcgc
    19141 gcgcgacctg ccgcgctcga tgcggctgca gcgcgcccac cgctgggagc gcttcccggc
    19201 gagcctgctc aaggacaaga cggtcgggat cttcggcgtc ggcgcgatcg cgctcgagct
    19261 cgcgccgaag tgcaaggcat tcgggatgcg cgtagtgggc atcacgtcgc atcggcgcga
    19321 agccccgggc ttcgacgcca tggtcgggcg cgacgcgctc gagcgcgcgg tgagcgagct
    19381 cgatttcctg gtgctcctga cgccgtactc ggaggccacg cgcaatatcg tgaacgcgag
    19441 tatcttcgcg gccatgaagc ccggcgcctg cctcgtcaac ctcgcgcgcg gcggcatcgt
    19501 cgacgaggcg gcgctggtcg aagccctgcg cgcaaagaag ctcgccgggg ccgcgctcga
    19561 tgtcttcgcg gaggagccgc tgcccgccga ttcgccgctc tgggacatgg acaacgtgct
    19621 cgtcacgcag caccagggcg gcttcttcga cgcttatccg cgctacgcgc tccccgtggt
    19681 cgaggagaac atgcgccggt ggctcgccgg cgacctcgcc ggcatggtca acgtcgtgag
    19741 gcactgatgg ccagcgagcg cctcaacgag cgtctgaact gcgcgatctc gacgcgcgag
    19801 ctcgagcggc gctgggccgc ggtgcgcgcc gccatgaccg agcgccgcat cgacgctctc
    19861 gtgatgcagg cgagcaacga ctggatgggc ggctacgtca aatggttcac cgacgtgccc
    19921 gccacgaacg gctatccggc gacggtcgtc ttcccgcgcg acgagcgcat gaccgtgatc
    19981 ggccagggac cgatgggcct ggagcgcgag ctgcccgccg agggcgacgg cgtaaggcgc
    20041 ggcgtcgcgc gcttcgtcgg gtcgcccgcg tacgcgtcgt gcccgtacac cgccgagtac
    20101 gacgcgcggc tcgcggagaa ggcgctcgcg aagtacgccg gcggcacgat cggcctcgtc
    20161 ggccgcggcg cgatgtcgtt cgcgttcggc gactacctgc agcgcaagct cagccgcgcc
    20221 aggttcgtcg acgcctcgga cctcgtcgat tggatcaagg cggtgaagag cgaggaggag
    20281 ctcgagctca tccgccgcac cgccgcgatg caggacgcgt gcatggaggc ggcgttccgg
    20341 cacatccatg ccggcatgcg cgacatcgag gtggccgcga tcgccgagca gatcgggcac
    20401 aagttcggaa gcgagcaggg gctgttcctc gcggcgtcgg cgccggtggg cgtatcggcg
    20461 ccgtttgcga accgctacca gcagaaccgc gtcctgcgcg agggcgacca gttcacgctg
    20521 ctgatcgaga acaacggccc gggcggcttc tacaccgagc tcggccgcac ctgcgtgctc
    20581 ggcaaggcct cgcaggagat gaaggacgag ttcgccttcg tgctcgaggc gcagaagttc
    20641 acgctctcgc tcatgcgccc gggcgcgaag tgcagcgaga tcttcgccga gtacaacgcc
    20701 ttcatgaagc gcagcaagcg gcccgaggag cggcgcctgc acgcgcacgg ccagggctac
    20761 gacatggtcg agcgtccgct cgtacgccac gacgagacga tgacgatcga gaagaacatg
    20821 aacatggtct gccacccgac ctacgtcacc gagcgcacct tcagctggtg ctgcgacaac
    20881 tggctgatcg gcgcctcggg cccggcggag aagctgcacc gcttcccgca ggagatcgtg
    20941 gagctgggat gaggagaaag cgatgaacag gaaactactc cggcccttca tcgtcacggc
    21001 gctcctggca gcggtcgagg tggcgcacgc gcagtcgtgg cccacgcgtc ccgtgaaggt
    21061 catcgtctcg caggccgcgg gcggcgcgcc cgacacgatc tgccggctgg tgacggaccg
    21121 cctcggaaat gcgctcggac agcagttcgt ggtcgacaac cgtccgggaa gcggcaacat
    21181 catcggcgct caggccgccg cgcgctcggc gcccgacggc tatacgctgt tcttcgcgac
    21241 ggcggccgcg ctcgtgacca atccgtacac cttcaagtcg ctgccctatg acccggtgaa
    21301 ggacttcgcg cccgtcgcca tggtcgccaa aggacccttc ttcgtgctcg cacacccgag
    21361 cgtgccggcg aagacgctgt ccgagctcgt cgcttacgac aaggccaacc ccggcaagct
    21421 cgcgttcg
//
LOCUS       DATDMW010000098         6267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2393251, whole genome shotgun sequence.
ACCESSION   DATDMW010000098 DATDMW010000000
VERSION     DATDMW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393251"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /gene="glgB"
                     /locus_tag="VI319_04250"
     CDS             <1..1477
                     /gene="glgB"
                     /locus_tag="VI319_04250"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY6863089.1"
                     /translation="NALDAPISVYEVHLGSWRRSPDTPHRVLGYRDRARALAADVKEM
                     GVTHVELMPVTEHPFYGSWGYQTTGYFAPTARYGTPQDFMYLVDVLHQNGIGVILDWV
                     PSHFPNDPHGLAEFDGTHVYEHADPRQGFHPEWGSCIFNYGRHEVRAFLASSALFWLD
                     RYHIDGLRVDAVASMLYLDYARKPGEWVPNVHGGHENLDAVTFLQQLNEAVYRHHPDV
                     QTIAEESTAWPAVSRPVYMGGLGFGMKWNMGWMHDTLSYFSKDPVHRKFHHGQLTFSA
                     WYAFTENFVLPLSHDEVVHGKGSLIGKMPGDDWQKFAGLRALFGYMWTHPGKKLLFMG
                     GEFGQRREWTHEQSLEWHVLDWAEHRGVRDWVRDLNRYHRSAPALYEQDFDSAGFEWI
                     DHNDAQNSVLSYIRKPRSHAPTVLVVCNLTPVPRWGYVLGVPRGGWWREVLNSDASCY
                     FGSGMGNAGGVHARAEAAHGRPFSLSLTLPPLSVVIFESES"
     gene            1581..3521
                     /locus_tag="VI319_04255"
     CDS             1581..3521
                     /locus_tag="VI319_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEY6863090.1"
                     /translation="MVEAITPMVDHGRFMAKRCAGDAVTVEADVFVDGHDVPRALLLH
                     RKQGGKRWNETPMSFLVNDRWRGEFPVPEIGVYEYTIAAWADPFASWRRDLARWTAAE
                     DVAVSLAAGAMIVGAAAERARGADAKRLEEWSERLAGGASLEDRRALANDPSLEALVA
                     AYPDRATITTFEPVLSLRVDPPLARFSSWYEMFPRSAAGGGRHGTFRDVEAQLAYVEA
                     LGFDVLYFPPIHPIGTTKRKGPNNAVVSGPGDPGSPWAIGSAEGGHKAIHPELGTAED
                     FRRLVRAARARGIEVALDIAFQASPDHPYVKAHPDWFRHRPDGSVQYAENPPKKYQDI
                     YPFDFESESWSALWHELRDVFLHWIAQGVRVFRVDNPHTKPFAFWEWAIAEIKREHPE
                     VIFLSEAFTRPKVMHRLAKLGFSQSYTYYAWRNAKGELTEYFTELSQSPSREYFRPNV
                     WPNTPDILTAALQTGGRAAFMSRLVLAATLSANYGIYGPAFELQEHVPLESGSEEYRD
                     SEKYQVRDWHLERSDSLRGLISKVNRIRRENPALHDDWRLRFHPVGNDQILCYSKTSA
                     DLSNAILVAVNLDPRNVQSGWVDLALGELGVDTPPFEVHDLLTDARYTWNGPRNYLEL
                     RPHDIPAHVFRVVPGTRISEQV"
     gene            3529..>6267
                     /gene="treS"
                     /locus_tag="VI319_04260"
     CDS             3529..>6267
                     /gene="treS"
                     /locus_tag="VI319_04260"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEY6863091.1"
                     /translation="MEMQNQEQLGQSEGPAQKAWRDDPLWYKDAIVYELHVKAFYDSN
                     GDGIGDFPGLTEKLDYIKELGVNTIWLLPFYPSPLKDDGYDISDYRGVHPSYGTLADF
                     RNFVREAHRRDLKIITELVINHTSDQHPWFQAARAAPAGSKKRDYYVWSDTAKKWEGT
                     RIIFTDTENSNWTWDPVAKAYYWHRFFSHQPDLNFDNPHVVRAVLKVMQYWFDMGVDG
                     MRLDAIPYLCERDGTSNENLPETHAVLRHMRRELDKHYKNRFFLAEVNQWPEDVREYF
                     GNGDECHMAYHFPLMPRIYMSVAEEDHYPIIDIMSQTPEIPDNCQWACFLRNHDELTL
                     EMVTERERDYMYRTYATDARARLNLGIRRRLAPLLQNNRRQIELVNSLLVSLIGSPII
                     YYGDEIGMGDNIYLGDRHGVRTPMQWSPDRNAGFSRADPQSLYLPPIMDPIYGYQAVN
                     VEAQARSPSSLLNWTRRMLTVSKAHVAFGRGSLRFVRVGNRKILAYIREYGDQTLLCV
                     ANLSRSSQPVELDLREYRTRIPVELLGRTPFPPIGELPYLLTLPAWGFYWFELATVAK
                     IPSWHDERPTRKEMPILVIPEGLRAILTSEAQTRNDLRSLMAQRQRQQLEREALPTFL
                     ASQRWFAGKGRDLAGTEITEHDEWSAPPGGNWLLTVAHVHYMDGTRQSYFLPVAIGWE
                     DGDYERLQALMHAAVARVRQRARVGILYDAFWDDAFCHSIVASMRSGEERRLPSGRLV
                     FWGTSAFVGMDPSGFAVSHPSFEQSNTMAILGNSLVLKGYRLVREGINPEIEIGKFLT
                     EVSPFPHIAPLMGALEHVGDDGRTFALAVLHKYVENQGSGWNYTIEYLGRYLEASLAA
                     TSLERTLGEPAAPGPAASVAPVAPGGDADSPHAAFLVLASTLGRRTGQMHVALAKHT"
BASE COUNT         1028 a         2252 c         2065 g          922 t
ORIGIN      
        1 gaacgcgctc gacgcgccga tctcggtgta cgaggtgcac ctcggctcgt ggcggcgcag
       61 cccggacacg ccgcaccgcg tcctcggcta ccgcgatcgc gcgcgcgcgc tcgccgccga
      121 cgtgaaggag atgggggtca cgcacgtcga gctgatgccg gtgaccgagc atcccttcta
      181 cggctcgtgg ggctaccaga ccaccggcta cttcgccccg accgcgcgct atgggacgcc
      241 gcaggacttc atgtatctcg tcgacgtcct gcaccagaac ggcatcggcg tgatcctcga
      301 ctgggtgcct tcgcatttcc cgaacgatcc gcacggcctc gcggagttcg acggcacgca
      361 cgtgtacgag cacgccgatc cgcgccaggg cttccatccc gagtggggaa gctgcatctt
      421 caactacggc cggcacgagg tgcgcgcctt cctcgcctcg agcgcgctct tctggctcga
      481 ccggtaccac atcgacggcc tgcgcgtcga cgcggtcgcg tcgatgctgt atctcgacta
      541 cgcgcgcaag cccggcgaat gggtgcccaa cgtgcacggg ggccacgaga acctcgacgc
      601 ggtcaccttc ctgcagcagc tgaacgaggc ggtctaccgg caccatcccg acgtgcagac
      661 gatcgccgag gagtcgaccg cgtggcccgc ggtgtcgcgc cccgtgtaca tgggcggcct
      721 gggcttcggc atgaagtgga acatgggctg gatgcacgac acgctgtcgt acttctcgaa
      781 ggacccggtg caccgcaagt tccaccacgg ccagctcacc ttcagcgcct ggtacgcatt
      841 caccgagaac ttcgtgctgc cgctctcgca cgacgaggtc gtgcacggca agggctccct
      901 gatcggcaag atgccgggag acgactggca gaagttcgct ggcctgcgcg cgctcttcgg
      961 ctacatgtgg acgcaccccg gcaagaagct gctgttcatg ggcggcgagt tcgggcagcg
     1021 gcgcgagtgg acgcacgagc agagcctcga gtggcacgtg ctcgactggg ccgagcaccg
     1081 cggcgtgcgc gactgggtgc gcgacctgaa ccgctaccat cgctcggcgc cggcgctcta
     1141 cgagcaggac ttcgattccg ccggcttcga gtggatcgat cacaacgacg cgcagaacag
     1201 cgtgctctcc tacatccgca agccgcgctc gcacgcgccc accgtgctcg tggtctgcaa
     1261 cctgacgccg gtgccgcgct ggggctacgt gctcggcgtg ccgcgcggcg gatggtggcg
     1321 cgaggtgctg aacagcgacg cgtcgtgcta tttcggcagc ggcatgggca acgcgggcgg
     1381 cgtgcacgcg cgcgcggaag ccgcgcacgg gcggccgttc tcgctcagcc tgaccctgcc
     1441 gcccctttcc gtggtgatct tcgaaagtga aagctaggag ccaggacacg ccgaccctca
     1501 cccccggccc tctcgctcaa ggaagagaga gggaggagac gcgcgcgctt ccgcgcgtcg
     1561 cggacggcaa gaaacgcgcc gtggtcgagg cgatcacccc gatggtcgac cacgggcgct
     1621 tcatggcgaa gcgctgcgcg ggcgacgccg tgaccgtcga ggccgacgtc ttcgtcgacg
     1681 gccacgacgt gccgcgcgcg ctgctgctcc accgcaagca gggcggcaag cgctggaacg
     1741 agacgccgat gagcttcctc gtgaacgacc gctggcgcgg cgagttcccg gtgccggaga
     1801 tcggcgtcta cgagtacacc atcgccgcct gggccgatcc gttcgcgtcg tggcggcgcg
     1861 acctcgcgcg ctggacggcg gcggaggacg tcgcggtgtc gctggcggcc ggcgcgatga
     1921 tcgtcggcgc ggccgccgag cgcgcgcgcg gcgccgacgc gaagcggctg gaggagtgga
     1981 gcgagcgtct cgcgggcggc gcgtcgctcg aggaccggcg cgcgctcgcg aacgatccct
     2041 cgctcgaggc cctggtcgcc gcctatcccg accgcgccac gatcacgacc ttcgagccgg
     2101 tgctctcgct gcgcgtcgat ccgccgctcg cgcgcttctc gagctggtac gagatgttcc
     2161 cgcgcagcgc cgcgggcgga gggcgtcacg gcacgttccg cgacgtcgag gcacagctcg
     2221 cgtacgtcga ggcgctcggc ttcgacgtgc tctacttccc gcccatccac ccgatcggca
     2281 cgaccaagcg caaaggcccg aacaacgcgg tcgtctcggg acccggcgat cccggcagcc
     2341 cgtgggcgat cggctccgcc gagggcggcc acaaggcgat ccatcccgag ctcggcacgg
     2401 ccgaggactt ccggcgcctg gtgcgtgcag cgcgcgcgcg cgggatcgag gtcgcgctcg
     2461 acatcgcgtt ccaggcctcg cccgaccatc cctacgtgaa ggcgcacccg gactggttcc
     2521 ggcaccggcc cgacggctcg gtgcagtacg ccgagaaccc gccgaagaaa taccaggaca
     2581 tctatccctt cgacttcgag agcgagtcgt ggtccgcgct gtggcacgag ctgcgcgacg
     2641 tgttcctgca ctggatcgcg cagggcgtgc gcgtgttccg cgtcgacaac ccgcacacca
     2701 agcccttcgc cttctgggaa tgggcgatcg ccgagatcaa gcgcgagcac cccgaggtga
     2761 tcttcctgtc cgaggccttc acgcggccga aggtgatgca tcgcctggcg aagctcggct
     2821 tctcgcagtc gtacacctac tacgcctggc gcaacgcgaa gggcgagctg accgagtatt
     2881 tcaccgagct gtcgcaatcg ccctcgcggg agtacttccg gccgaacgtc tggccgaaca
     2941 cgcccgacat cctcacggcg gcgctgcaga cgggcgggcg cgcggcgttc atgtcgcggc
     3001 tcgtgctcgc ggcgacgctg tcggcgaact acggcatcta cggccccgcg ttcgagctgc
     3061 aggagcacgt gccgctcgag agcggcagcg aggagtaccg cgactccgag aagtaccagg
     3121 ttcgcgactg gcacctcgag cgctccgaca gcctgcgggg gctgatctcg aaggtgaacc
     3181 ggatccggcg cgagaatccc gcgctgcacg acgactggcg cctgcgcttc cacccggtcg
     3241 gcaacgacca gatcctctgc tactcgaaga ccagcgccga cctctcgaac gcgatcctcg
     3301 tcgcggtcaa cctcgacccg cgcaacgtgc agtcgggctg ggtcgacctc gcgctgggcg
     3361 agctcggcgt cgacacgccg ccgttcgagg tccacgacct cctcaccgac gcgcgctaca
     3421 cctggaacgg cccgcgcaac tatctcgagc tgcggccgca cgacattccc gcgcacgtct
     3481 tccgggtcgt tcccggtacc cgcatttcgg aacaggtcta ggccgctcat ggaaatgcag
     3541 aaccaggagc agctgggaca atccgaaggc ccggcgcaga aggcgtggcg cgacgatccg
     3601 ctctggtaca aggacgcgat cgtctacgag ctgcacgtca aggcgttcta cgactcgaac
     3661 ggcgacggca tcggcgactt tcccgggctc accgagaagc tcgactacat caaggagctc
     3721 ggggtcaaca cgatctggct ccttccgttc tatccctcgc cgctcaagga cgacggctac
     3781 gacatctccg actaccgcgg cgtgcacccg agctacggca cgctcgccga tttccgcaac
     3841 ttcgtgcgcg aggcgcaccg gcgcgacctg aagatcatca ccgagctcgt catcaaccac
     3901 acctccgacc agcatccctg gttccaggcg gcgcgcgcgg cgcccgccgg ctcgaagaag
     3961 cgcgactact acgtctggag cgacacggcg aagaagtggg aaggcacgcg catcatcttc
     4021 accgacaccg agaactcgaa ctggacctgg gacccggtgg cgaaggccta ctactggcac
     4081 cgcttcttct cgcaccagcc cgacctcaac ttcgacaacc cgcacgtcgt gcgcgcggtg
     4141 ctcaaggtga tgcagtactg gttcgacatg ggcgtcgacg ggatgcgcct cgacgcgatc
     4201 ccgtacctgt gcgagcgcga cggcaccagc aacgagaacc tgcccgagac gcacgcggtg
     4261 ctgcggcaca tgcgccgcga gctcgacaag cactacaaga accgcttctt cctcgcggaa
     4321 gtgaaccagt ggcccgagga cgtgcgcgag tacttcggca acggcgacga gtgccacatg
     4381 gcgtaccact tcccgctgat gccgcgcatc tacatgtcgg tggccgagga ggaccactac
     4441 ccgatcatcg acatcatgtc gcagacgccg gagatcccgg acaactgcca gtgggcgtgc
     4501 ttcctcagga accacgacga gctcacgctc gagatggtga ccgagcgcga gcgcgactac
     4561 atgtaccgca cctacgccac cgacgcgcgc gcgcgcctca acctcgggat ccgccggcgc
     4621 ctcgcgcccc tgctgcagaa caaccggcgc cagatcgagc tggtgaacag cctgctcgtg
     4681 tcgctgatcg gctcgcccat catctactac ggcgacgaga tcggcatggg cgacaacatc
     4741 tacctcggcg accgccacgg cgtccggacg ccgatgcagt ggagcccgga ccgcaacgcc
     4801 ggcttctcgc gcgcggatcc ccagagcctc tacctgccgc cgatcatgga cccgatctac
     4861 ggctaccagg cggtgaacgt cgaggcgcag gcgcgcagcc cctcgtcgct gctcaactgg
     4921 acgcggcgca tgctgacggt gagcaaggcg cacgttgcgt tcgggcgcgg ctcgctgcgc
     4981 ttcgtgcgcg tgggcaaccg caagatcctc gcctacatcc gcgagtacgg cgaccagacg
     5041 ctgctctgcg tcgcgaacct ctcgcgctcg agccagccgg tcgagctcga cctgcgcgag
     5101 taccgcaccc gcatcccggt cgagctgctc gggcgcacgc cgtttccccc gatcggcgag
     5161 ctgccctacc tgctgacgct gcctgcgtgg ggcttctact ggttcgagct ggcgacggtc
     5221 gcgaagatcc cgtcctggca cgacgagcgc ccgacgcgca aggagatgcc gatcctggtc
     5281 attcccgagg gattgcgcgc gatcctcacc tcggaggcgc agacgcgcaa cgacctgcgc
     5341 agcctgatgg cgcagcgcca gcgccagcag ctcgagcgcg aggcgctgcc caccttcctc
     5401 gcctcgcaac gctggttcgc gggcaagggc cgcgatctcg ccggcaccga gatcaccgag
     5461 cacgacgagt ggagcgcccc gcccgggggc aactggctgc tgaccgtggc gcacgtgcac
     5521 tacatggacg gcacgcgcca gagctacttc ctgcccgtcg cgatcgggtg ggaggacggc
     5581 gactacgagc ggctgcaggc gctgatgcac gccgcggtgg cgcgcgtgcg ccagcgcgcc
     5641 cgcgtcggca tcctctacga cgctttctgg gacgacgcct tctgccactc catcgtcgcg
     5701 agcatgcgct cgggcgagga gcgccggctg ccgagcgggc gcctcgtgtt ctggggcacg
     5761 agcgccttcg tcggcatgga cccgagcggc ttcgcggtga gccacccgtc cttcgagcag
     5821 agcaacacca tggcgatcct cgggaactcg ctcgtgctca agggctaccg gctggtgcgc
     5881 gagggcatca accccgagat cgagatcggc aagttcctca ccgaggtctc gcccttcccg
     5941 cacatcgcgc cgctcatggg cgcgctcgag cacgtcggcg acgacggccg caccttcgcg
     6001 ctcgcggtcc tgcacaagta cgtcgagaac cagggcagcg gctggaatta cacgatcgag
     6061 tatctcggcc gctacctcga ggcgagcctc gccgcgactt cgctcgagcg gacgctgggc
     6121 gagccggccg cgccggggcc cgcggcgagc gtcgctccgg tcgcgccggg cggcgacgcc
     6181 gacagcccgc acgccgcctt cctcgtgctc gcgagcacgc tcggacggcg cacgggacag
     6241 atgcacgtcg cgctcgccaa gcacacc
//
LOCUS       DATDMW010000099        27016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_400684, whole genome shotgun sequence.
ACCESSION   DATDMW010000099 DATDMW010000000
VERSION     DATDMW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 27016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27016
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400684"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..156
                     /gene="rpmH"
                     /locus_tag="VI319_04265"
     CDS             19..156
                     /gene="rpmH"
                     /locus_tag="VI319_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY6863092.1"
                     /translation="MKRTLHPSVRRRKRTHGFLVRMRTRGGRAVIRARRAKGRARLGV
                     V"
     gene            153..482
                     /gene="rnpA"
                     /locus_tag="VI319_04270"
     CDS             153..482
                     /gene="rnpA"
                     /locus_tag="VI319_04270"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY6863093.1"
                     /translation="MSDTRRYSREQRLRGEDIEALLAAGKGLRRSGVWVQARVNALEC
                     PRLGMIIPRRVVPRAVDRNRIRRVLREWFRLNRERLGSRDILVRVTDKPVELVSDVVA
                     QLLAKLP"
     gene            479..709
                     /gene="yidD"
                     /locus_tag="VI319_04275"
     CDS             479..709
                     /gene="yidD"
                     /locus_tag="VI319_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY6863094.1"
                     /translation="MKTVLSTLLRAYRYAVSPMLGLQCRFHPSCSAYALEAIEHWGAL
                     RGSWLAIRRLARCHPWHPGGHDPVPHCEQENA"
     gene            734..2353
                     /gene="yidC"
                     /locus_tag="VI319_04280"
     CDS             734..2353
                     /gene="yidC"
                     /locus_tag="VI319_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY6863095.1"
                     /translation="MDTQRLILFFVFSFSLLLLWEAWQKESRPPPAPAAAAAPTGVPG
                     PAASKAGSAAKPETVPGAGAEAPGELLTVRTDTMLARVSTTGGDIVYLELLQHKSTLD
                     STKNFVLFDKDHRYAAESGLTGGLPYHRSVFAASAKEFELAPGADKVDVRLEARTPSG
                     VRVVKTLTFRRSSYFIDVGEDIVNGSSAPVSTTAYFQLTRDDKAPAGDPNMVKTFTGA
                     AFYTNQDKFQKVAFSDIDKGKFTAKSADNGWAAMVQHYFVAALLPGDKVQREFYAQKL
                     GDDFYSVGVKIPVGPIEAGGTASVTVPMYAGPEYQDKLKAIAPGLDRVVDYGMLTIIA
                     EPLFWVLKVFHGWLANWGLAIILLTICIKLLFFPLSAASYRSMAKMKLVTPRLAKLRE
                     QYADDRMKMNQAMMELYKTEKINPLGGCLPIVVQIPVFISLYWVLLASVELRHAPFYG
                     WITDLSAEDPYYVLPALMMASMLLQTRMNPTPPDPVQAKVMLIMPFVFGVMFFFFPSG
                     LVLYWLVNNILSIAQQWQITRMIEAKKPPKR"
     gene            2409..3752
                     /gene="mnmE"
                     /locus_tag="VI319_04285"
     CDS             2409..3752
                     /gene="mnmE"
                     /locus_tag="VI319_04285"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041491300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY6863096.1"
                     /translation="MSPDTDVIAAVATAQGRAGIGIVRISGRKLQPFVSALLGRRPPP
                     RSAVRAVFRGADGRPIDEGLALYFAAPSSYTGEDVLELHGHGGPVVLRMLLRRCIELG
                     ARLAEPGEFTRRAFLNDKLDLAQAEAVADVIDAATETAARCALRSLRGEFSQLVRGFA
                     DQLLELRALVEATLDFPEEDIDAQHRLQAEVRLEGLREQLRRALEQARRGSVLRAGIQ
                     VVLAGQPNVGKSSLLNKLAGEDVAIVTDIPGTTRDAVRQAIQVEGVPMNIIDTAGLRD
                     TTDTVERFGIARTWDAIERADVLLLVVDARTGVSPEDVRILERLPPKLARVIVFNKID
                     LCGVPAASEESDGERRIRLSAMTGEGVDLLRAAMVSTAGWEATNEDVFMARERHVVCL
                     ERAAGALERAAAAMARIELFAEELRIAQCALDAITGETTPDDVLGEIFSRFCIGK"
     gene            4049..5881
                     /locus_tag="VI319_04290"
     CDS             4049..5881
                     /locus_tag="VI319_04290"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY6863097.1"
                     /translation="MNQPYTPGVDIAAPNYVRHQPVKEWVARIAALTKPERVVWCDGS
                     QDEYDRLCDEMVKKGTMKRLNQKLRPGCFLALSDPSDVARMEDRTFICSRQKEDAGPT
                     NNWMDPGEMRVKLDQLFDGSMRGRTMYVVPYSMGPLGSHIAHIGIELTDSPYVVVSMR
                     VMTRMSKKVWDILDENGPFVPGVHSVGAPLTRGESDVSWPCNKEHKYIVHYPETKEIW
                     SFGSGYGGNALLGKKCFSLRIASVMGREQGWLAEHMLILGVTSPDGRKTYVSGAFPSA
                     CGKTNFAMLIPPPAFNGWKVTTIGEDIAWLKPGKDGRFYAINPEAGFFGVAPGTSNKT
                     NPNCMKMIQSNTIFTNVAMTPEGDVWWEGMTETPPARLTDWTGKEWTPDCGRKAAHPN
                     SRFTVSAAQCPSIDPEWENPDGVPISAFMFGGRRSDTVPLVMESFDWDSGVYLAGTMG
                     SETTAAATGKVGVVRRDPMAMIPFIGYNVGDYFNHWLKLGRSVPNRPRIFCVNWFRTD
                     ANGKFIWPGFGENMRVLKWIVERCQGKAHAVKTPVGFVPEFGDLEWKGLEGFGNEQFA
                     KVTSIDPNAWRQELKYHDQILGIIGSRLPGELAAKRDALGQVFR"
     gene            6125..8017
                     /gene="mnmG"
                     /locus_tag="VI319_04295"
     CDS             6125..8017
                     /gene="mnmG"
                     /locus_tag="VI319_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY6863098.1"
                     /translation="MRFPGHFDVIVVGGGHAGTEAALASARAGCRTLLLTHSIETLGQ
                     MSCNPSIGGIGKGHLVREVDALGGAMALATDEAGIQFRVLNSSKGPAVRATRAQADRL
                     LYKQAIRMRLENQPNLTLFQQAVDDLVLEAGHVAGVVTQLGIRFYARAVVLTTGTFLS
                     GKVHVGLDNYQAGRAGDPASLALAGKLRELGLPVGRLKTGTPPRIDGRSIDFSVMKEQ
                     PGDKPAPCFSFLGMPDVHPRQVPCWITHTNSRTHDIIRAGLDRSPMYTGVIEGVGPRY
                     CPSIEDKIHRFAGKESHQIFLEPEGLTTHEFYPNGISTSLPFDVQVDLVHSINGLEEA
                     HVLRPGYAIEYDYYDPRGLKSSLETRAIPGLFFAGQINGTTGYEEAAAQGLLAGINAA
                     LRVRDRAAWCPRRDEAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLMLREDNADLRLTE
                     VGRKLGVVDDQRWDAFNRKRDAIARESERLKSTWVGPKTIDADAAEQLLGAPLERDYT
                     LLDLLKRPDVRYGELMKLVGGSATQLDTAVTEQVEIQAKYSGYIERQREEVERRERHD
                     SMPLPSDLDYRTVRGLSIEVQQKLNAHKPETLGQASRISGVTPAAISLLLVHLKRAMQ
                     AQKSAA"
     gene            8014..8670
                     /gene="rsmG"
                     /locus_tag="VI319_04300"
     CDS             8014..8670
                     /gene="rsmG"
                     /locus_tag="VI319_04300"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY6863099.1"
                     /translation="MSTEADTAPATDPKKQLDRGLGELGLELDRNARKRLLAFAELLE
                     KWNRVYNLTAIRGADQIVSGHLLDCLAIVPHIPSTGRALDVGSGAGFPGIPLALARPR
                     MQVTLLDSNQKKAAFLRQAVGELQLDNATVIEDRVEAWQPQEKFDVVVSRALTDTGEF
                     VRWSRHLLAPGGVFAAMKGVVPRDEFEGLPQGYRVRDTVRLTVPGLEAQRHLILVERA
                     "
     gene            8672..9451
                     /locus_tag="VI319_04305"
     CDS             8672..9451
                     /locus_tag="VI319_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6863100.1"
                     /translation="MTRILAITNQKGGVGKTTTSVNLAAGLAQSGKRVLLVDLDPQGN
                     ATMGSGIDKRALSATVYQVLLGFATAPSVRQRSEAGGYDIIPANRDLAGAEVEMVDLE
                     HRESRLKSALQAIAADYDFVLLDCPPSLNMLTLNGLVAADAVMIPMQCEYYALEGLSD
                     LVNTIKKVRQHLNPRIEIEGLLRTMYDPRNTLAQQVSAQLQEHFGAKVYETVIPRNVR
                     LAEAPSFGAPAISFDRECKGSQAYMALASEILNRNPLAAAA"
     gene            9448..10326
                     /locus_tag="VI319_04310"
     CDS             9448..10326
                     /locus_tag="VI319_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY6863101.1"
                     /translation="MNMATRPKGLGRGLDALLDKENAGGAASGEVQATVAVGRLERGR
                     YQPRTNMDEGSLQDLAASIKSQGLMQPILVRPVNGTREEPRYEIIAGERRWRAAKIAG
                     LAEVPVLVRNVPDSAALAMALIENIQREDLNPLEEANGVRRLVEEFNLTHQQAAEAIG
                     RSRAATTNLLRLLQLQKSVQQLVLDGQLEMGHARALLALEGRRQEDTAKSVAERGLSV
                     RQTEQLVNEILNPPAIKAKPRPRNDRDVERLEEELSGELGTSVEIRPGKKGTGKLVIH
                     YASPEHLDDLLSRLKG"
     gene            complement(10334..12391)
                     /locus_tag="VI319_04315"
     CDS             complement(10334..12391)
                     /locus_tag="VI319_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6863102.1"
                     /translation="MSSGRLRALAALFLASVWLSRPDYALAQESPDEVRVTGHYENAV
                     GTSDAASQGNITPKLLEARPMQRPAEVLEYVPGVIITQHSGEGKANQYFLRGFNLDHG
                     TDFATTVDHMPVNMPTHAHGQGYSDLNFLIPELVSRISYKKGPYFADEGDFASAGAAN
                     INYATKLKAPIASLGLGNDGWKRAMAANSFDSGPGHLLYGLELFHNDGPWDNPSDYNK
                     VNGVLSYSVGTNADGYTLTGMAYSGKWNATDQIPQRAVDSQQVSRFGAIDPSDGGQTS
                     RYSLSLDRHRSWSNGDFHMNTYFIKYRLRLFSNFTFFLDDPVNGDQFEQFDDRKVYGV
                     DPTWTFTGKWGERQVTNRFGADLRYDDIGTVALYHTVERERLATVREDKVGQTGAGLF
                     YENTVQWNDWVRTIAGLRTDYYRARVDSSVDANSGSSRDHATSPKLNFVFGPFDHTEY
                     FLNLGQGFHSNDARGTTIAVDPTTGAPAEKVKPLVKTRGEELGVRTEVVPGLQSSLAL
                     WRLRLDSELVFVGDAGTTEASRPSLRRGIEWSNRYVPRPWVIVDLDLSASRANFRGDG
                     PAGNYIPGAIDKVASLGVTLNDIGRWSYTAHLRYFGPRPLVEDNSVRSPSSTLVSLRT
                     SYRIDPRFRINFDVLNLLNRKVDDIDYFYASQLRNESAPVNDIHFHPAEPRSFRIALV
                     GQF"
     gene            12556..13791
                     /locus_tag="VI319_04320"
     CDS             12556..13791
                     /locus_tag="VI319_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY6863103.1"
                     /translation="MQTANDARVPWPEEGVARIPYWVYTDPRIYAREQERIFCGPSWS
                     YVALEAEIPNAGDFKRTFIGEKPVVVVRDKDGGINVVENRCAHRGVQFCQKHLGHATE
                     FMCPYHQWTYDLKGNLVGVPFRRGLKKQGGMPADFDLKAHGLTKLRVARRHGVVFASF
                     ATNVEALEDYLGETILGLFDRVFDGRALRVLGYSRQMIPANWKLMFENIKDPYHASLL
                     HVFLVTFGLFRADNPSRVVTDPTGRHGALLSWKGEQKRTADNADMKSLIEDFRLEDPS
                     MLEPVREFAQYTVAMTTLWPNLIIQQQSNTLAMRQLVTRGPEAFELAWTFFGYESDDE
                     AMTRRRLRQANLMGPAGYVSVDDSEIMMFSQGGLRHRPDAAAVMEMGGRDWRPAEEHM
                     VTESAIRGFYDYYRKIMEV"
     gene            13838..14338
                     /locus_tag="VI319_04325"
     CDS             13838..14338
                     /locus_tag="VI319_04325"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY6863104.1"
                     /translation="MTAGQRYLSMNASELRLALEELYAEYIGCLDEERFEAWPEFFTD
                     PCLYKIVPRENFERGLPLATWLCESRGYLRDRVAAIRKTAMYAPRTLRRMVSAIRILG
                     WRGETLEVRANYLALETLQDELTRVFNTGQYHDMLVVDDGRLRFREKVCVFDSALVPT
                     SMIYPL"
     gene            14357..15337
                     /locus_tag="VI319_04330"
     CDS             14357..15337
                     /locus_tag="VI319_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HEY6863105.1"
                     /translation="MEKLRLSIALDEYDHTRDVMSGRVPVEGVELVPMNLQIEEIFYR
                     TTFYREWDISELSMGKYCSLRSQGDETFTAIPVFVSRQFRLSMVYVRDDGKITRPEQL
                     AGKRVGVPEWAQTATVYSRGWLAHDCGVKLESIDWVQAGLQTPGRVEKVKLNLPQGLK
                     LRPAPQGTLNDMLLAGELDAILTARAPRAVGKGIVRLIPDYRPVEEAHYRRTGIYPIM
                     HVVVLRTEVLARHPWVAGNLLKAFEEAKRRSYERLDQIALSAVPLAWIYDYVGQMKAI
                     FGDDFFPYGVEKNRRTLDAFLQYGHEQGVFHRRLQPEDLFPKQVLSTFKV"
     gene            15345..16343
                     /locus_tag="VI319_04335"
     CDS             15345..16343
                     /locus_tag="VI319_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY6863106.1"
                     /translation="MTTDPLWITESEVVALLDLKDAIVALEDALRKEASGDAQNMTKT
                     LLQYGKSNLHAIGGKLGELVGTKTWTHTPGGTCPLLLLWSATDGSLVAVIEAFALGNM
                     RTGGISGVAADWMAKPDARVFALIGTGKQALAQAATMIAVRPIERLQVYSPRTETRQA
                     FAVKAREEFGIEVVECASAADACRGAQIVTLVTRAAQPFLTARMLDAGAHLNAVGAIA
                     PDREEFAQDVFDRATLVAVDNLPGVQQLSRELMTRYGARGWDDVTPLSRLIANGRRRT
                     AGDDVTLFKAMGMGISDLALGAELVARARRRGIGRTIAQPKKVKPRLTRAQTKSAA"
     gene            16372..17496
                     /locus_tag="VI319_04340"
     CDS             16372..17496
                     /locus_tag="VI319_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEY6863107.1"
                     /translation="MNEFRKDFERLEGRFEDRFGAPARDENRYWPPIIVGKEQIDAEV
                     ERLAAQPLPANGRRESLIVHPLAAPNAPGFAPGIQVKLSVLKPGESSAPFRHNATEVN
                     FCIRGSGHALVAGRRVAYEQYDVWNTPGYAIYRHVNDGKDLAVRLTYSNAPLLQHLQV
                     YLPEDDPATGLKEVHEDEDKRNDPRRRNPYGMFPITDDGGLLMPYETLINPQPVESRP
                     LHFAWKRVKPELDKLEALGKDYIGRRLYMYYNPVTGRTNGITPNFFATMTIRPPKIVD
                     RPHRHASAAINYYFQGRGYSMVAGNRYEWQAGDLMLSAPGWAVHNHASYDDYVYELTV
                     QDQPLHIWMESLLWQEDLKGSIALLGTHAGFETNRSKVAA"
     gene            17549..18829
                     /locus_tag="VI319_04345"
     CDS             17549..18829
                     /locus_tag="VI319_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY6863108.1"
                     /translation="MAETIVKARKRGDYLSRSEALEILRIKPQTLYCYVSRGYIRSVP
                     QPGGRTSYYLREDVERVKAKSVARSGHGPAAASAMRWGEPVIESSITEITDRGPRYRS
                     RLAVDLARSGATFESVAEYLWTGDWHEGARGWHVEDRLGELPNLLTTRVKLFPEVHIL
                     QLFSGAAVIRGFAPVSAQHKQPGAAPACASRLIRTMTGAFGFIGPQRAFTALEAGESV
                     AHGLARALGIPTDPARLRALDAALVLIADHELNPATFVARVAASAGSSLHACVQAALG
                     VWVGSAIGLTCDQLEQMFAPGVAADEALARARSTLRAGSKLPGFDLPVYPNGDPRAET
                     LIDIAREVDPAAVECMLDAVQRIEGELEVRPVVEIGLVMLCRALGAPAGAAGGIFALG
                     RVAGWVAHVLEQREQSFLIRPRAQFARSAARALS"
     gene            18852..19649
                     /locus_tag="VI319_04350"
     CDS             18852..19649
                     /locus_tag="VI319_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6863109.1"
                     /translation="MRMPDYQVTGEGKVTVFLLHGMYGAKDYWRYQTERLVARGYRVV
                     AWDAPGYGLSPLPATFSFDVLAEAAAQLIRATGGERNVVFGHSMGGQITPRIRLKIPE
                     LVHGIVISATIGYFGNRTKEEQEEFVRKRSAPPPPGTDPVTAQLAVVNSMLGPKSAGP
                     EVELVRWIASRTPPKSVQAAVKAVQSYPEADAVAAIRAVSVPTLLVAGEVDQTGHAAG
                     MKRVADMIEGAAFAVVPGSGHYPWAENPEEFNRAFFGFLDRHFPARG"
     gene            complement(19654..20634)
                     /locus_tag="VI319_04355"
     CDS             complement(19654..20634)
                     /locus_tag="VI319_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863110.1"
                     /translation="MLTRRSLVVVALAAALGTVVGQAAAQAWPSRPMRIIVPLPPGGS
                     PDYMARLLSEKLQPVLGQPLVVENKPGASGHIARDFVAKAPADGYTLLMTEASHVISA
                     AVGAKLPYDPINDFQPIALIATIPFGLTVNSKTPVHTLREFIDYAKASPRPLTYGTAG
                     VGAPHHFAVELLRAETGIKVTHVPYKGSAGIIPALLSGEIDFTIAAVNSLLPHFKSGK
                     LRPIAMAGSARTSVLPEIPTIAEAGPFPGYSMDVWLGVMAPAGTPRPIIDRLNAEINR
                     IVRDPQIVKERMNTVGLEPLGSTPEKMLEVMKTELAKYRKVARDADIKAD"
     gene            complement(20646..22433)
                     /locus_tag="VI319_04360"
     CDS             complement(20646..22433)
                     /locus_tag="VI319_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY6863111.1"
                     /translation="MNAKENLAANAVRHDTPVAGAEPKEFWGSDAVAAMLRALGIPYL
                     ALNPGASYRGLHDSIVNYLGNSAPQMLLCLHEEAAIHIAQGYTKASGKMMGAVVHSNV
                     GLMHATMAIFNAWCDRVPMLVLGATGPWDAAKRRPWIDWIHTATDQGALVRGFTKWDN
                     QPGSALAAYEALLRAAQIANTAPRGPTYINLDAALQEQKIGALPPVPDPARFRAPDPV
                     IPAPHLVEQAAKLLSQAKHPVILAGRVSRSESGWKQRVALAEKLQARVFTDIKIAAAF
                     PTDHPLHAAPPSAFHSPVVAKMIREADVVLSLDAVDLAGTLKAAWGAEAVGAKVINVS
                     PDAHIHRGWSMDYQGLPPADVYLMCEPDAAVPLLLDAVKARPAAAPAPKPELPPADAK
                     VVSLRTLADAFNAATEGLDVSITRVPLGWNGAYRHFRHPMDFLGSEGGGGVGASVGNT
                     VGAALALKGSGRLPVAITGDGDFLMGVTGLWTAAHYGIPCLIIVANNRSFFNDELHQE
                     RVAIERSRPVQNRWIGQRIGDPDIDLAAMARAQGAQGIGPVTETKDLLPAIRKGIDAV
                     RAGAVCVIDVRVQPGYDTELKSSSATSRK"
     gene            complement(22504..23556)
                     /locus_tag="VI319_04365"
     CDS             complement(22504..23556)
                     /locus_tag="VI319_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6863112.1"
                     /translation="MASAGRDPLAGKLPVTLITGFLGSGKTTLLRRLLRDPAMNRAAV
                     IINELGEVGIDHELVAQSSESMTLLENGCLCCAVRTDLQETLRELFIRRRAGEVIDFD
                     RVFVETTGLADPVPVLHTLQSDGLLGAQYRLDGVVTLVDAVNGAATLDRAPEAPRQVA
                     VADRIVVTKTDLAPPATVAALHDRLEAMNPYAARTEAINGELDPELLRSIGPRSARAN
                     VDEVSRWLAPSGSPDVTGQGAYLGKAVRAVHDPSIRSFVLWFDKPFTWDSFHAAVQAL
                     TSLRGPDLLRVKGLINVQGERGPVVVQGAQHVFHPPVTLESWSSEERRSRLVFITRGV
                     ERDAVASLFAAVGALA"
     gene            complement(23556..24794)
                     /locus_tag="VI319_04370"
     CDS             complement(23556..24794)
                     /locus_tag="VI319_04370"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863113.1"
                     /translation="MPSTRIGPRADQTLALNMRLLSHHELQGFGGIGEGMGMQLTRGR
                     RILWLAHESAPKNFTAVDVTDPRKPHVVVQTELPHAKVRSNSLDVVGDVMAVAYQTKE
                     VGLKPAGFELFDISDPEKPRVISHFDCSGPHSRGVHAVWFVDGETVHMAAGAADFQPT
                     HPNDDQCYRIVDVKNTSSPREVGRWWFPGTRVGDDVPPPARLPKIDAGYRAHNTNVYP
                     ERPDRAYVGYIDGGAVILDIADKSRPKLVANWRYSPPYNGFCHTVMPLLDRGLLIVSD
                     ECIKNDGADWPKLVWVVDAREEKNPVPISTFPLPPHETFTKRGGRFGAHNLHENLPVP
                     GSWRSQEIVVGTFFSGGVRAFDVGNPYQPQEVAYYVPGTPNGSPVGSAQLNDVYVDDR
                     GLVYTVDRFIGGLYILEMNI"
     gene            24917..25885
                     /locus_tag="VI319_04375"
     CDS             24917..25885
                     /locus_tag="VI319_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863114.1"
                     /translation="MHRLIRSTIGWVAAFACGAALAQTPAYPNHPVKLIVTYPPGGSS
                     DLMARVFGAKLAEHWGQQVITENKPGAAGSIGMEYAARQPADGYSFVVGNIGPAAVNP
                     LLTKVPYSMDRDFVAISMLSTGPNILIVHPDVPAKSLAELTSYAKANAGKMNYGTSGP
                     GSISQLVGAMYKNIAGVSATEVPYKGGILAVQDVVAGHLQYIFSDTLPAMQFIKSGRV
                     RPLCTTGAKRSDILPEYPACAEAIPGLVAINWWGVFMPAGTPKAITDKFHGDLVKVLQ
                     DPDVKKKFADLGVEAVSSTPEELVRFMKSETAKYSKLIQEAGIKAE"
     gene            complement(25890..26783)
                     /locus_tag="VI319_04380"
     CDS             complement(25890..26783)
                     /locus_tag="VI319_04380"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6863115.1"
                     /translation="MKIGVVGIGRMGSAITTRLLGLGHEVTVWNRTPDKTRPLVAAGA
                     TSAATPAAVATRSEAVISIVSDAQAIASAYEGLQGLLHGEPAGKLFIEMSTVRPDVQR
                     ALAPKVRAAGAAFVECPVGGTVGPAREGKLFGFVGGEPEDVARARPLLEQLCRRVEHV
                     GPVGAGATVKLAINLPLLVYWQALGEALALCGPLDIEQTRLMDIFADTSGGTNAVKGR
                     GPAIAAVMKGGAPGPVASDVELARKDLAAMIEEAEALGVKLPVSASALECYDAAVREG
                     RGGSDMSLVPVELYAKTRRRR"
BASE COUNT         4516 a         8872 c         9240 g         4388 t
ORIGIN      
        1 ccttgaggaa gacgtttcat gaaacgcact ttgcacccct cggtgcgtcg ccggaagcgc
       61 acgcacggat ttctggtacg gatgcgcacc cgcggaggcc gcgcggtgat ccgcgcgcgc
      121 cgcgcgaaag ggcgcgcgcg cctcggggtc gtttgagcga cacgcggcgc tattcccgcg
      181 aacaaagact gcgcggcgag gacatcgagg cgctgctagc ggcaggcaag ggcctgaggc
      241 gctcgggcgt ctgggtgcag gcccgagtca atgcgctcga gtgcccgcgc ctgggcatga
      301 tcattccgcg gcgcgtcgtg ccgcgagccg tcgaccgcaa tcgaatccgc cgtgtgctgc
      361 gagaatggtt ccgactgaat cgagagcgcc tgggaagccg cgacattctc gtacgagtga
      421 ccgataagcc cgttgagctc gtgagcgacg tggtggcgca actgctggcg aaacttccgt
      481 gaagaccgtg ctctccacgc tgctgcgagc gtatcgatac gccgtgagcc cgatgctcgg
      541 gctgcagtgc cgatttcacc cgtcgtgctc ggcatacgcg ctcgaggcga tcgagcattg
      601 gggcgcgctc cgcgggagct ggctcgccat taggcgcctc gcccgatgcc atccgtggca
      661 tcccggcggc catgatcccg tcccacattg cgaacaagaa aacgcctgaa acgatctcga
      721 ctgcgaactg cggatggata cccagcggct gatccttttc ttcgtctttt cgttttcgct
      781 gctcctgctt tgggaggcgt ggcaaaagga gagtcggccg ccgcccgcgc cagcagccgc
      841 ggcagcgccg accggtgtcc ccgggcctgc ggcttcgaaa gccggtagcg cggccaagcc
      901 cgagacggtc ccgggcgcgg gcgccgaggc gcccggtgag ttgctcacgg tgcgcacaga
      961 cacgatgctg gcccgcgtga gcacgacggg cggggacatc gtctatctcg agcttctgca
     1021 gcacaagagc acgctcgaca gcacgaagaa cttcgtgctg ttcgacaagg accaccgcta
     1081 cgcggcggag agcgggctca cgggagggct gccttatcac cgctcggttt tcgcggcgtc
     1141 ggcgaaggaa ttcgagctcg ccccgggcgc cgacaaggtg gacgtcaggc tcgaagcccg
     1201 gaccccttcc ggggtgagag tggtgaagac gctgacgttc cgccgttcga gctacttcat
     1261 cgacgtcggc gaggacatcg tcaacggctc ctccgcgccc gtctccacca cggcttattt
     1321 ccagctgacg cgagacgaca aggcgcccgc gggcgatccc aacatggtga agaccttcac
     1381 cggcgcggcg ttctacacga atcaggacaa gttccagaaa gtcgccttca gcgacatcga
     1441 caagggcaag tttacggcca agtcggccga caacggctgg gccgcgatgg tgcagcacta
     1501 cttcgtcgca gcgctgctgc ccggggacaa ggtgcagcgc gagttctacg cgcagaagct
     1561 gggtgacgac ttctattcgg tcggcgtgaa gatcccggtg ggacccatcg aggcgggtgg
     1621 cacggccagc gtcacggtgc cgatgtacgc gggccccgag tatcaggaca agctgaaagc
     1681 gattgcgccc gggctcgatc gggtcgtcga ttacggcatg ctcaccatca tcgccgagcc
     1741 cctgttctgg gtgctcaagg tcttccatgg ttggctcgcc aactggggtc tcgccatcat
     1801 tctcctgacc atctgcatca agctgctctt cttcccgctg tcggccgcga gctaccgctc
     1861 gatggccaag atgaagctcg tcacgccgag gctcgccaag ctccgcgagc aatacgccga
     1921 cgaccgcatg aagatgaacc aggcgatgat ggagctctac aagacggaga agatcaaccc
     1981 gctcggcggc tgcctgccga tcgtcgtcca gattcccgtg ttcatctcgc tctactgggt
     2041 gttgctcgcg agcgtcgagc tgcgccacgc gccgttctac ggctggatca ctgacctgtc
     2101 ggccgaggac ccatattacg tgttgcccgc gctgatgatg gcgtccatgc tgctgcagac
     2161 gagaatgaac ccgacgccgc cggatcccgt ccaggcgaag gtcatgctga tcatgccgtt
     2221 cgtattcggc gtgatgttct tcttctttcc gtcgggcctg gtgctctatt ggctcgtgaa
     2281 caacatcctg tcgattgccc agcagtggca gatcacccgc atgatcgaag ccaagaagcc
     2341 gcctaagcgc tgaccgacat cgcgtacgga ggcgccgtcc gttcgcgggc aggcggcttc
     2401 ccgcagacat gtcccccgac accgatgtga tcgccgccgt tgccaccgcg caaggtcgcg
     2461 cgggcatcgg tatcgtccgc atctcgggga ggaagctcca gcctttcgtg agtgccctgc
     2521 tcgggaggcg gcccccgccg cggtccgcgg tgcgcgcggt gttccgggga gcggacgggc
     2581 gaccgatcga cgagggtctc gccctttact tcgccgcgcc gtcctcctat acgggggaag
     2641 acgtcctcga gcttcacggc cacggcggcc cggtggtgct gcggatgctc ttgcggcggt
     2701 gcatcgagct cggagcgcgc ctcgcggagc ccggcgagtt cacgcgacgc gcgtttctga
     2761 acgacaagct ggatcttgcg caggccgaag ccgtcgcgga cgtgatcgac gctgcgacgg
     2821 agaccgccgc gcgttgcgcg ctgcgctcgc ttcgcgggga gttctcacag ctggtccgcg
     2881 gcttcgcgga ccagctgctg gagctgcggg cgctggtgga ggcgacgctc gactttccgg
     2941 aggaggacat cgacgcccaa caccgtctcc aggccgaggt gcggctcgaa ggtctccgcg
     3001 agcaattgcg ccgggcgctc gagcaggcgc gccggggcag cgtgttgcgc gcgggcatcc
     3061 aggtggtgct cgcggggcag ccgaacgtcg gcaaatcgtc cctgctcaac aagctcgccg
     3121 gcgaagatgt ggccatcgtg accgatatcc ccggaacgac gcgagacgcg gttcgccagg
     3181 cgatccaagt cgaaggcgtg ccgatgaata tcatcgacac cgctggcttg cgcgacacga
     3241 ccgacacggt cgagcgattt ggcattgccc gcacctggga cgcgatcgag cgggccgatg
     3301 tgctgctgct cgtcgtcgac gcgaggacgg gcgtctcccc tgaggatgtc cgcatcctcg
     3361 aacggctgcc cccgaagctc gcgcgcgtca tcgtgttcaa caagatcgat ctgtgcggcg
     3421 tgccggccgc atcggaggag tcggacggcg agcgccgtat tcgcctgtcg gcgatgaccg
     3481 gcgagggcgt cgacttgctg cgcgccgcaa tggtttcgac ggccggatgg gaggcgacga
     3541 acgaagatgt cttcatggcg cgcgagcgcc atgtggtctg cctcgagcga gcagccgggg
     3601 ccttggagcg cgcggccgcc gccatggcgc ggatcgagct cttcgcggaa gagctgcgca
     3661 tcgctcagtg cgcgctcgac gcgatcaccg gcgagaccac gcccgacgac gtgctcggcg
     3721 agatcttctc ccggttctgc atcggtaaat gatgccgacg agcgccgcgt gccgttccga
     3781 acgcgcagcg cgcgttcgcc catgcaccgg cgaacctcac ggcacctggg gtgtcattac
     3841 gtacgccggc ttgctatggt agagtcgcgc ctctactcgt cgatcagcca cgacttccgg
     3901 ttcgccccat cgcccggaag cggcgaaaac ttctcttttt ttgcgctgcg aggctgctgt
     3961 ttttgaatgg caatgcccta gcttttccgg ctctcgatgc tgcgatgcaa aacgcgcgag
     4021 agctcaaatc ccgaggaaga ggccgtagat gaaccaaccg tataccccag gcgtcgatat
     4081 cgctgctccc aattacgtcc ggcatcagcc ggtgaaggag tgggtggcgc gaatcgcagc
     4141 actcaccaaa ccggaacgcg tggtttggtg cgacggctcg caggacgagt acgaccgtct
     4201 ctgcgacgag atggtcaaga agggtacgat gaagcggctc aaccagaagc tgcgtccggg
     4261 ttgcttcctc gcgctgtcgg acccgagtga tgtcgcgcgc atggaggata ggacctttat
     4321 ctgcagcagg cagaaggaag acgccgggcc gacgaacaac tggatggatc cgggcgagat
     4381 gcgagtcaag ctcgaccagc tgttcgacgg gagcatgcgc ggacgcacga tgtacgtcgt
     4441 gccctattcg atggggccgc tcggatcaca catcgcgcac atcggcatcg agctcacgga
     4501 ttcgccgtac gtggtcgtga gcatgcgggt catgacgcgc atgtcgaaga aggtctggga
     4561 catcctcgac gagaacgggc ctttcgtgcc gggcgtgcat tcggtcgggg cgccgctcac
     4621 gcgcggagaa agcgacgtgt cctggccgtg caacaaggaa cacaagtaca tcgttcatta
     4681 ccccgagacc aaggagatct ggtcgttcgg atcgggctac ggcggcaatg cgctgctcgg
     4741 gaagaagtgc ttctcgctgc gcatcgcgtc ggttatggga cgagagcaag gctggctcgc
     4801 agagcacatg ctcatcctcg gtgtgacgtc gcccgacgga cgcaagacgt acgtgagcgg
     4861 cgcattcccg tcggcgtgcg gcaagacgaa tttcgcgatg ctgatcccgc cgccggcgtt
     4921 caacggctgg aaggtgacca cgatcggcga ggacatcgcc tggctcaaac cgggcaagga
     4981 cggtcgtttc tacgcgatca atccggaagc cgggttcttc ggtgtggcac cgggaacgtc
     5041 gaacaagacg aacccgaatt gcatgaagat gatccagagc aacaccatct tcacgaacgt
     5101 cgcgatgacg cccgagggtg acgtgtggtg ggagggaatg acggagactc ctcctgccag
     5161 gctgaccgac tggaccggca aagagtggac gccggactgc ggtcgcaagg ccgcgcatcc
     5221 gaactctcgc ttcacggtgt ctgcggctca atgcccgtcg atcgatcccg agtgggagaa
     5281 ccccgatggc gtgccgatct ctgcgttcat gtttggaggc cggcggtcgg acacggtgcc
     5341 gctcgtcatg gaatcgttcg actgggattc gggcgtctat ctcgccggca cgatgggatc
     5401 ggaaaccacg gcggccgcca ccggcaaggt cggtgtcgtc cggcgcgatc cgatggccat
     5461 gattccgttc atcggctaca acgtgggcga ctacttcaat cactggctca agctcggacg
     5521 ttcagtgccg aaccgtcctc gcatcttttg cgtcaactgg ttccgcacgg acgccaacgg
     5581 caaattcatc tggcccggct tcggcgagaa catgcgcgtg ctgaagtgga tcgtcgagcg
     5641 ttgccaaggc aaggcgcacg cggtgaaaac gccggtcggc ttcgtgccgg aattcggcga
     5701 cctggaatgg aaaggtctcg agggcttcgg aaacgagcag ttcgccaagg tgacctcgat
     5761 agatcccaat gcctggcgcc aggagctgaa gtaccacgat cagatcctcg gcattattgg
     5821 ctcgcgcttg cccggcgaat tggcggccaa gcgagacgcg ctgggacagg tctttcgctg
     5881 aaggtctacg aggcgcataa aggccgcctc agggcggcct ttttatttat attcagcatc
     5941 ttgcgactaa ttctcattag tggatgaggc agtggattac caggggggcg ttcgtgcaca
     6001 agctgtgatg ttccacgtgg aaccacgact ttaggacgcc cgctaatcgt agaggtcgcg
     6061 gctgctttcg ccgcgacgcg cgcaaaaata ccctacaatc agcgccgaag agcaggtgat
     6121 ctgaatgcga ttccccggtc atttcgatgt catcgtggta ggcggcggtc acgctgggac
     6181 cgaagccgcg ctggcttcgg ctcgcgccgg gtgccgaacg ctgctgctga cccacagcat
     6241 cgagacgctg gggcaaatgt cctgcaatcc gtcgattggc gggattggaa aagggcatct
     6301 cgtcagagag gtggacgccc ttggcggtgc gatggcactc gcgacggacg aagcagggat
     6361 ccaattccgc gttctgaatt cgtcgaaagg gcctgcggtc cgggcaacgc gtgcgcaggc
     6421 tgaccggctc ctctacaagc aggcgatcag aatgcgcctc gagaaccagc caaacctgac
     6481 gcttttccag caggcggtcg acgatctcgt gctcgaagcg ggtcatgtgg cgggcgtggt
     6541 gacccagctc ggcatccggt tttacgctcg agcggtggtc ctgacgacgg gtaccttcct
     6601 ttccgggaag gtccacgtcg gtctcgacaa ctatcaggct ggtagggcgg gggacccggc
     6661 gtctttggcc ctcgcgggga aactgcgcga gctcgggctc ccggtcggcc gattaaagac
     6721 tggcacgcct ccacgcatcg atggacgcag catcgatttt tccgttatga aggagcaacc
     6781 cggcgacaag cccgcgccgt gcttttcctt cctcgggatg cccgatgtgc atccgcgaca
     6841 agtgccgtgc tggatcacgc acaccaactc ccgcacgcac gacatcatcc gcgccgggct
     6901 cgatcgttcg ccgatgtata cgggagtcat cgaaggcgtc ggcccgcgct attgcccctc
     6961 catcgaggac aagatccatc gttttgccgg aaaagagagt caccagatct tcctggagcc
     7021 cgagggtctg accacccacg agttctatcc caacggcatt tcgaccagcc tgccgttcga
     7081 tgttcaagtt gatttagtcc attcaatcaa tggattagag gaagcgcacg tcctgaggcc
     7141 ggggtacgcg atcgagtacg actattacga cccccgaggc cttaagtctt cgctggagac
     7201 gagggccatc ccggggcttt tcttcgcggg tcagatcaat ggcaccactg gctacgagga
     7261 agccgctgca caaggtctgc tcgcgggcat caatgccgcc ctgcgggtcc gagaccgagc
     7321 cgcctggtgt ccacggcgcg acgaggccta tctcggggtg ctcgtggatg acctggtcac
     7381 gcgcggtgtg agtgagccgt accgcatgtt cacgagccgc gcggagtatc gcctgatgct
     7441 gcgcgaggac aacgccgacc tgcgcttgac cgaggtcggt cgcaagctcg gcgtggtgga
     7501 tgatcagcgt tgggatgcgt tcaaccgcaa acgcgatgcc atcgcgcgcg aatcggagcg
     7561 attgaagtcg acctgggttg gaccgaaaac gatcgacgcc gacgccgcgg agcagctgct
     7621 cggagcaccg ctcgagcgcg attacacttt gctcgatctc ctgaaacggc ccgacgtgcg
     7681 ctacggcgag ctgatgaaac tcgttggggg cagcgcgacg caactcgaca ccgcggtcac
     7741 cgagcaggtg gagatccaag ccaaatacag cggctacatc gagcggcagc gggaggaggt
     7801 cgaacggcgc gagcgccacg attcgatgcc tttgccgagt gaccttgact accggacggt
     7861 gcgcggcctg tcgatcgaag tgcagcagaa gttgaacgcc cacaagccgg aaacactggg
     7921 acaggcctcg cgaatctcgg gcgtcacgcc ggccgccatt tctttgctgc tggttcatct
     7981 gaagcgcgcg atgcaagcgc agaagagcgc cgcatgagca ccgaagccga taccgcgcct
     8041 gcgaccgacc ccaagaagca gctcgaccgc ggcctcggag agctcggcct cgagctcgac
     8101 cgcaacgcgc gtaaacggct gctcgcgttt gccgaactgc tcgagaagtg gaaccgggtc
     8161 tacaacctga ccgccatacg gggcgctgac cagatcgtaa gcgggcattt actcgattgc
     8221 ctcgccatcg tgccccacat cccctccacc gggcgggcac tggatgtcgg cagcggcgcc
     8281 ggcttccccg ggatcccact ggcgctcgca cggccgcgca tgcaggtgac gctgctcgac
     8341 tcgaaccaga agaaggcggc gttccttcgt caggcggttg gcgagctgca gctcgacaac
     8401 gcgaccgtga tcgaggaccg ggtcgaggcc tggcagcccc aggagaagtt cgatgtcgtc
     8461 gtctcgcgcg cgctcaccga caccggcgag ttcgtccgct ggtcgcgaca cctgctcgcg
     8521 ccgggtggcg tgttcgctgc gatgaaaggc gtcgtcccgc gcgacgagtt cgaaggcctt
     8581 ccccagggct atcgcgtgcg cgataccgtg cgccttacgg ttccggggct ggaggcgcag
     8641 cggcatctca tcctcgtgga gcgcgcatga catgactcgc atattggcga tcacgaacca
     8701 gaagggcggc gtcggcaaga cgaccacgag cgtgaatctc gccgcgggct tggcgcagtc
     8761 cggcaagcgc gtgctgctcg tggatttgga tcctcagggc aacgccacca tgggtagcgg
     8821 catcgacaag cgcgctctca gcgcgaccgt gtaccaggtc ctgctcggct tcgcgaccgc
     8881 gccctcggtg cggcagcgat cggaagccgg cggctacgac atcatcccgg cgaaccgcga
     8941 tctcgccgga gcggaagtgg agatggtgga cctcgagcac cgcgagtcgc ggctcaagtc
     9001 ggcgctgcag gcgatcgcgg cggactacga cttcgtcctg ctcgattgcc cgccgtcgct
     9061 caatatgctc acgctcaacg gcctcgtcgc ggcggacgcc gtcatgatcc cgatgcagtg
     9121 cgagtactac gcgctcgagg gtctgtccga tctcgtaaac acgatcaaga aggttcgcca
     9181 gcatctgaat ccgaggatcg agatcgaggg actgctgcgc acgatgtacg atccgcgcaa
     9241 cacgctcgcg cagcaggttt ccgctcagct ccaggagcac ttcggggcaa aagtgtacga
     9301 gacggtcatt ccgcgcaacg tccggctcgc cgaggcgccc agcttcggcg cgcccgcgat
     9361 cagcttcgac cgggagtgca aaggctcgca agcctacatg gcgctcgcga gcgagatcct
     9421 gaaccgcaac ccactggcgg ccgccgcgtg aacatggcga cccgacccaa gggcctcggt
     9481 cgggggctcg acgcgctact cgacaaggag aacgcgggcg gggcggcgtc gggcgaggtg
     9541 caagcaaccg tcgccgtcgg gcgcctcgag cgcgggcgct atcagccccg cacgaacatg
     9601 gacgaaggat cgcttcagga tctcgcggcc tcgatcaagt cgcagggcct gatgcagccg
     9661 atcctcgtgc gcccggtgaa cggaacccgc gaggagccgc gctacgagat catcgccggc
     9721 gagcgccgct ggcgggcggc gaagatcgcg gggctcgccg aggttccggt cctggtgcgc
     9781 aacgtgcccg attcggcggc gctcgcgatg gcgctgatcg agaacatcca gcgcgaggac
     9841 ctgaatccgc tcgaggaagc gaacggcgtc cggcgcctcg tcgaagagtt caatctcacg
     9901 caccagcagg ccgccgaggc gatcgggcgg tcacgcgctg cgacgacgaa cctgctgcgc
     9961 ctgctgcagt tgcagaagtc tgtgcagcag ctcgtgctgg atgggcagct ggagatggga
    10021 cacgcgcggg cgctgctggc actcgagggt cgccgccagg aagacaccgc gaagagcgtc
    10081 gcggagcgcg gcctctcggt gcgccagacc gagcagctcg tgaacgagat cctcaatcct
    10141 cccgcgatca aggcgaagcc gcggccgcgc aacgaccgtg atgtcgagcg cctggaagag
    10201 gagctctccg gcgagctcgg cacgagcgtg gagatccgcc ccgggaagaa agggacgggc
    10261 aagctcgtga tccactatgc cagccccgag cacctcgacg acctgctttc gcggctgaaa
    10321 ggctaggccg ccgctagaac tggccgacta gggcgatgcg gaagctgcgc ggctctgccg
    10381 gatggaaatg gatatcgttc acgggcgccg actcgttgcg cagctgcgac gcgtagaagt
    10441 aatcgatgtc gtccactttc cggttcaaca ggttcagcac gtcgaaattg atccggaacc
    10501 ggggatcgat ccggtacgag gtgcgaagcg acacgagcgt tgacgatggc gagcgcacgc
    10561 tgttgtcctc gaccagcggc cggggaccga agtagcgcag gtgtgcggtg tacgaccagc
    10621 gtccgatgtc gttcagcgtg acgccgagcg aggccacctt gtcgatggcg cccggaatgt
    10681 aattgccggc aggaccgtcg ccccggaaat tggcgcgcga cgcggagaga tccaggtcga
    10741 cgatcaccca cggtctcggg acgtagcggt tcgaccattc gatgccgcgg cgcaggctcg
    10801 ggcggctcgc ctcggtcgtc ccggcgtcgc cgacgaacac cagctccgaa tccaggcgca
    10861 gccgccagag cgcgagcgag ctctgcagtc cgggaacgac ttccgtgcgc acgcccagct
    10921 cctcgccgcg cgtcttcacc aggggcttca ccttctccgc aggagcacct gtcgtcggat
    10981 cgaccgcgat ggtcgtgccg cgcgcgtcgt tgctgtggaa gccctggccg agattcagga
    11041 agtactcggt gtggtcgaag ggcccgaaga cgaaattgag cttgggcgag gtggcgtggt
    11101 cgcgactcga gcccgagttc gcgtccacgc tgctgtcgac gcgggcgcga tagtagtcgg
    11161 tgcgcagccc ggcgatggtc cgcacccagt cgttccactg aaccgtgttc tcgtagaaga
    11221 ggccggcgcc cgtctggccg accttgtcct cgcgcacggt cgccaggcgc tcgcgctcga
    11281 cggtatggta aagcgccacg gtgccgatgt cgtcgtagcg caagtcggcg ccgaaccggt
    11341 tcgtgacctg gcgctcgccc cacttgcccg tgaacgtcca ggtcggatcc acgccgtaca
    11401 ccttgcgatc atcgaactgc tcgaactggt cgccgttcac cgggtcgtcg aggaagaagg
    11461 tgaagttcga gaacagtcgc aggcggtact tgatgaagta cgtattcatg tgaaagtcgc
    11521 cgttcgacca cgaccggtga cggtcgagcg agaggctgta gcgcgacgtc tggccgccgt
    11581 cgctcgggtc gatcgcgccg aagcgcgaga cctgctgcga atcgacggcg cgctgtggga
    11641 tctggtcggt cgcgttccat ttgccgctgt aggccatgcc cgtgagcgtg tagccgtcgg
    11701 cgttggtgcc gacgctgtag ctcaggactc cgttcacctt gttgtagtcg gacgggttgt
    11761 cccacgggcc gtcgttgtgg aagagctcca ggccatagag cagatggccg ggcccggagt
    11821 cgaacgagtt cgccgccatc gcccgcttcc agccatcgtt gccgaggccg aggctggcga
    11881 tcggggcctt gagcttggtc gcatagttga tattggccgc gcccgcggaa gcgaaatcgc
    11941 cttcgtcggc gaaatacggt cccttcttgt acgagatgcg cgagaccagc tcggggatga
    12001 ggaagttgag atcggaatag ccctgaccgt gggcgtgtgt cggcatgttg accggcatgt
    12061 ggtcgaccgt cgtcgcgaag tcggtgccgt ggtcgagatt gaagcctcgc aggaagtact
    12121 gattcgcttt cccttcgccg ctgtgctgcg tgatgatcac gccggggacg tactcgagga
    12181 cctcggcggg ccgctgcatc ggccgcgctt cgagcagctt cggcgtaatg ttcccctggc
    12241 tcgccgcgtc cgacgtgccg acggcatttt cgtagtggcc cgtgacgcgg acttcgtcgg
    12301 ggctctcctg cgccagggcg taatccgggc gcgagagcca aaccgaggca agaaaaagcg
    12361 cagcgagcgc gcgaagcctg cccgacgaca tcgtctctcc ctgaaagccg ccggagcccg
    12421 cggctcgaag ccgcggatta tctccgtttg atcatgccgc cgccacacac cgggccgggt
    12481 tcgttcgcct gccatgcagg acgtggccgg cgtgccgctg ctatgatccg gtcgaaaccg
    12541 cttgatccgg atcacatgca gaccgcgaac gacgcgcgcg tgccctggcc cgaggaggga
    12601 gtcgcgcgga tcccgtactg ggtgtacacc gacccgcgca tctacgcgcg cgagcaggag
    12661 cggattttct gcggcccgag ctggagctat gtcgcgctcg aagcggaaat cccgaacgcc
    12721 ggcgacttca agcgcacgtt catcggcgag aaacccgtgg tcgtcgtccg agacaaggac
    12781 ggtgggatca atgtggtcga gaaccgctgc gcccaccgcg gcgtgcagtt ctgccagaaa
    12841 catctcgggc acgcgaccga gttcatgtgc ccgtatcacc agtggacata cgacctcaag
    12901 ggcaacctcg tgggcgtgcc gttccgccgc gggttgaaga agcagggcgg tatgccggcc
    12961 gacttcgacc tgaaggcgca cggcctcacg aagcttcgcg tcgcgcgccg gcacggggtc
    13021 gtgttcgcct cgttcgccac gaacgtcgag gcgctcgagg actatttggg cgagacgatc
    13081 ctcgggctgt tcgaccgcgt cttcgacggc cgcgccctgc gcgtcctcgg gtattcgcga
    13141 cagatgatcc cggcgaactg gaagctgatg ttcgagaaca tcaaggaccc gtaccacgca
    13201 agcctgctgc acgtgttcct ggtgaccttc ggcctgttcc gcgccgacaa tccctcgcgc
    13261 gtcgtgaccg atccgaccgg ccggcacggc gcgctgctgt cgtggaaggg cgagcagaag
    13321 cgcaccgcgg acaacgccga catgaaatcc ctgatcgagg acttcaggct cgaagatccg
    13381 tcgatgctcg agccggtgcg cgagttcgcg cagtacacgg tcgcgatgac gacgctctgg
    13441 cccaatctca tcatccagca gcagtcgaac acgctcgcca tgcggcagct cgtgacccgc
    13501 gggcccgagg cgttcgagct cgcatggacc ttcttcggct acgagagcga cgacgaggcg
    13561 atgacgcggc gccggttgcg ccaggcgaac ctcatgggcc cggcgggtta cgtctcggtc
    13621 gacgacagcg agatcatgat gttttcgcaa ggcggcctgc ggcaccggcc cgacgcagcg
    13681 gcggtgatgg aaatgggagg ccgggactgg cgacctgccg aggagcacat ggtcacggaa
    13741 tcggcgatcc gaggcttcta tgactactat cggaagatca tggaagtatg atatttcatg
    13801 gttttatctc aataattcga acgttcgttc atatcgtatg actgccggtc agcgctatct
    13861 ttcgatgaac gcctcggagc tgcgcctggc cctggaggag ctgtacgccg agtacatcgg
    13921 ctgtctcgac gaggagcgct tcgaggcgtg gcccgagttc ttcaccgatc cgtgcctgta
    13981 caagatcgtg ccgcgcgaga acttcgagcg cggcctgcct ctcgcgacct ggctgtgcga
    14041 gagccgcggc tacctgcgcg accgcgtcgc cgcgatccgc aagacggcga tgtacgcgcc
    14101 gcggacgctg cggcgcatgg tgagcgcgat ccgcatcctc ggctggcgcg gcgagacgct
    14161 cgaggtgcgc gccaactacc tcgcgctcga aaccctgcag gacgagctca cgcgggtatt
    14221 caacaccggg cagtaccacg atatgctcgt ggtcgacgac ggccgcctgc gctttcgcga
    14281 gaaagtatgc gtcttcgaca gcgcgctcgt tccgaccagc atgatctacc cactctgacc
    14341 gagaatcttc caccccatgg aaaagctaag gctcagcatc gcgctcgacg aatacgacca
    14401 cacccgcgac gtgatgtcgg gccgcgtccc ggtcgagggc gtcgagctcg tgccgatgaa
    14461 cctgcagatc gaggagatct tctaccgcac gaccttctat cgcgagtggg acatctccga
    14521 gctgtcgatg ggcaagtact gctcgctgcg ctcgcaaggc gacgagacct tcaccgcgat
    14581 cccggtgttc gtgtcgcggc agttccggct gtcgatggtg tacgtgcgcg acgacggcaa
    14641 gatcacgcgg cccgagcagc tcgccggcaa gcgcgtcggc gtccccgaat gggcgcagac
    14701 ggcgaccgtc tacagccgcg gctggctcgc gcacgactgc ggcgtgaagc tcgagtcgat
    14761 cgactgggtg caggcgggcc tgcagacgcc cggccgcgtg gagaaggtga agctgaatct
    14821 tccgcaggga ttgaagctgc gacccgcgcc gcagggtacg ctgaacgaca tgctgctcgc
    14881 gggcgagctc gacgcgatcc tcacggcgcg ggccccgcgc gcggtcggca aagggatcgt
    14941 gcgcctcatt cccgattacc ggcccgtcga ggaggcgcac taccgccgca ccggcatcta
    15001 cccgatcatg cacgtcgtcg tgctgaggac cgaggtcctc gcgcgccatc cctgggtggc
    15061 gggcaatctc ctgaaggcgt tcgaggaggc gaagcgccgc agctacgagc ggctcgacca
    15121 gatcgcgctg tccgcggtcc cgctcgcctg gatctacgac tacgtcgggc agatgaaggc
    15181 gatcttcggc gacgatttct ttccgtacgg ggtcgagaag aaccggcgca cgctcgacgc
    15241 gttcctgcag tacggtcacg agcagggcgt gttccatcgc cggctgcaac ccgaggacct
    15301 gttcccgaag caggtgctct ccacgttcaa ggtgtgaaag ccctatgacg accgacccgc
    15361 tctggatcac cgaaagcgaa gtcgtcgcgc tgctggacct caaggacgcg atcgtggcgc
    15421 tcgaggatgc gctgcgcaag gaagcctcgg gcgatgcgca gaacatgacg aagacgctgc
    15481 tgcagtacgg caagagcaac ctgcacgcga tcggcggcaa gctgggcgag ctggttggca
    15541 ccaagacctg gacccatact ccgggcggga cgtgcccgct gctcctgctg tggagcgcga
    15601 ccgacggctc gctggtcgcg gtcatcgaag ccttcgcgct cgggaatatg cgcaccggcg
    15661 gcatttcggg ggtcgccgcc gactggatgg cgaagcccga cgcgagggtc ttcgcgctga
    15721 tcggcacggg caagcaggcg ctcgcgcagg cggcaaccat gatcgccgtg cgccccatcg
    15781 agcggctgca ggtctacagc ccgcgcacgg agacgcgcca ggcgttcgcg gtgaaggcgc
    15841 gcgaggagtt cggcatcgag gtggtcgagt gcgcgtcggc ggccgacgcg tgccgcggcg
    15901 cgcagatcgt gacgctggtg acgcgcgcag cgcagccctt cctcaccgcg cgcatgctcg
    15961 atgcgggcgc gcatctcaac gccgtggggg cgatcgcgcc cgaccgcgag gagttcgccc
    16021 aggatgtgtt cgaccgcgcc acgctggtgg cggtggacaa tctgccgggc gtgcagcagc
    16081 tatcgcgcga gctgatgacc cgctacgggg cccgcggctg ggacgacgtg acgccgcttt
    16141 cgcggctgat cgcgaacggg cgccgccgca ccgccggcga cgacgtgacg ctattcaagg
    16201 cgatgggcat ggggatctcc gacctcgccc tgggggccga gctggtggct cgcgcgcgcc
    16261 ggcgcggcat cgggcgtacc atagctcaac cgaagaaggt aaagccccgg ctgacgcgag
    16321 cgcaaacgaa atcggccgca tgaaggtcta aacctcgtcc gaggaggctg catgaacgaa
    16381 tttcgcaagg atttcgaaag gctcgagggc cggttcgagg accggttcgg cgccccggcg
    16441 cgcgacgaga accggtactg gccgccgatc atcgtcggca aggaacagat cgacgccgag
    16501 gtcgagcgcc tcgcggcgca gccgctgccg gcgaacggca ggcgcgagtc gctcatcgtc
    16561 catccgctcg ccgcgccgaa cgcgcccggc ttcgccccgg gaatccaggt gaagctctcg
    16621 gtgctcaagc cgggggagtc gagcgcgccc ttccgccata acgccaccga ggtcaacttc
    16681 tgcatccgcg gctcgggcca cgcgctcgtc gccggccggc gcgttgccta cgagcagtac
    16741 gacgtctgga acaccccggg ctacgcgatc taccgccacg tgaacgacgg caaggacctc
    16801 gctgtgcgcc tcacgtactc gaacgcgccg ctgctgcagc acctgcaggt gtacctcccc
    16861 gaggacgacc cggccaccgg gctgaaggaa gtgcacgagg acgaggacaa gcgcaacgac
    16921 ccgcgccggc gcaatcccta cggcatgttc ccgatcaccg acgacggcgg gctgctgatg
    16981 ccgtacgaga cgctgatcaa cccgcaaccg gtggagtcgc gcccgctgca cttcgcgtgg
    17041 aagcgcgtga agcccgagct cgacaagctc gaagcgctcg gcaaggacta catcggccgg
    17101 cggctgtaca tgtactacaa ccccgtgacc ggccgcacca acggcatcac gccgaacttc
    17161 ttcgcgacga tgacgatccg tccgccgaag atcgtcgacc ggccgcatcg ccatgcgtcg
    17221 gcggcgatca actactattt ccagggacgc ggctactcga tggtcgccgg caaccgctac
    17281 gagtggcagg cgggcgatct catgctgtcg gcgccgggct gggccgtgca caaccacgcc
    17341 tcgtacgacg actacgtgta cgagctcacg gtgcaggacc agcccttgca catctggatg
    17401 gaatccctcc tgtggcagga ggatctgaag ggatcgatcg cgctccttgg cacgcacgcc
    17461 ggcttcgaga ccaaccgctc gaaggtggcc gcgtgagagg cgtcggagtt ttcagctgaa
    17521 cgagtccacc atgcccgggg aggggcatat ggccgaaacc atcgtaaagg cgcgaaagcg
    17581 cggcgactat ctatcgcgta gcgaggccct ggagatcctg cggatcaagc cgcagacgct
    17641 ctactgctac gtgagccgcg gctacatccg cagcgtcccg caaccgggcg ggcgcaccag
    17701 ctactacctg cgcgaggacg tcgagcgcgt caaggcgaag agcgtcgcgc gctcgggcca
    17761 cgggcccgct gcggcctccg cgatgcgctg gggcgagccg gtgatcgagt cctcgatcac
    17821 cgagatcacc gaccgcgggc cgcgctaccg cagccgcctg gccgtcgacc tggcgcgctc
    17881 gggggcgacc ttcgagtcgg tcgcggaata cctctggacc ggcgactggc acgagggcgc
    17941 gcggggctgg cacgtcgagg accggctcgg cgagcttccg aatctgctca cgacgcgcgt
    18001 caagctgttt cccgaggtgc acatcctgca attgttctcg ggcgcggcgg tcattcgggg
    18061 attcgcgccc gtgagcgcgc agcacaagca gcccggcgct gcgccggctt gcgcgagccg
    18121 cctgatccgg accatgaccg gcgccttcgg cttcatcggc ccgcagcgcg cgttcaccgc
    18181 gctcgaggcc ggcgagtcgg tcgcgcacgg cctggcgcgc gcgctcggca tcccgaccga
    18241 cccggcgcgc ctgcgcgcgc tcgacgccgc gctcgtgctc atcgccgacc acgagctgaa
    18301 cccggcgacc ttcgtcgcgc gcgtcgcggc gtccgcggga tcgtcgctgc acgcctgcgt
    18361 gcaggccgcc ctcggcgtct gggtcgggtc ggcgatcggg ctgacgtgcg accagctcga
    18421 gcagatgttc gcccccggcg tcgccgccga cgaggcgctc gcgcgcgccc gctcgacgct
    18481 gcgtgcgggc agcaagctgc cggggttcga cctgccggtg tatcccaacg gcgacccgcg
    18541 tgccgagacg ctgatcgata tcgcgcgcga ggtcgacccc gcggcggtgg agtgcatgct
    18601 cgacgcggtg cagcgcatcg aaggcgagct cgaggtgcga cccgtcgtcg agatcggcct
    18661 ggtgatgctg tgccgcgcgc tgggtgcgcc cgccggggcc gccggcggca tcttcgctct
    18721 gggacgcgtc gcgggatggg tcgcgcacgt cctcgagcag cgcgagcagt cgttcctgat
    18781 ccgcccgcgc gcgcaattcg cacgctccgc ggcgcgcgcg ctctcgtaga cgctttttcg
    18841 cagggatccg catgcgcatg cccgactacc aggtgaccgg cgagggcaag gtcacggtct
    18901 tcctgctgca cgggatgtac ggcgccaagg actactggcg ctaccagacc gagcggctgg
    18961 tcgcgcgcgg ctaccgcgtg gtcgcctggg atgcgcccgg ctacggcctg tccccgttgc
    19021 cggcgacatt cagcttcgac gtgctggccg aagcggcggc gcagctcatc cgtgcgacgg
    19081 gcggcgagcg caatgtcgtc ttcgggcaca gcatgggcgg ccagatcacg ccgcgcatcc
    19141 ggctgaagat ccccgagctc gtgcacggca tcgtgatctc cgcgaccatc ggctacttcg
    19201 gcaaccgtac caaggaggag caggaagaat tcgtgcgcaa gcgcagcgcg cctccgccgc
    19261 cggggaccga tccggtcacg gcgcagctcg cggtcgtcaa ctcgatgctc gggccgaagt
    19321 cggccggccc ggaggtggag ctcgtgcgct ggatcgcgtc gcgcacgccg ccgaagtcgg
    19381 tgcaggcggc ggtgaaggcg gtgcagagct atcccgaagc cgacgcggtc gctgcgatcc
    19441 gcgcggtctc ggtgccgacg ctgctggtcg cgggcgaagt cgaccagacg ggccacgcgg
    19501 cgggcatgaa gcgcgtcgcg gacatgatcg aaggcgccgc gttcgccgtt gtcccgggct
    19561 cgggccacta tccgtgggcc gagaaccccg aggaattcaa tcgcgcgttc ttcggttttc
    19621 tcgaccggca cttccccgcg cggggatagg cggtcagtcc gccttgatgt cggcgtcgcg
    19681 tgcgaccttg cggtacttcg cgagctcggt cttcatgacc tcgagcatct tctccggcgt
    19741 gctgccgagc ggctcgaggc cgaccgtgtt catgcgctcc ttcacgatct gcgggtcgcg
    19801 cacgatgcgg ttgatctcgg cgttcaggcg atcgatgatc ggacgcggcg tcccggccgg
    19861 cgccatgacg ccgagccaca cgtccatcga gtagcccggg aacgggcccg cctcggcgat
    19921 cgtcgggatc tcgggaagca ccgacgtgcg cgccgatccc gccatcgcga tcgggcggag
    19981 cttccccgac ttgaaatgcg gcagcagcga attgaccgcg gcgatggtga agtcgatctc
    20041 acccgagagc agcgccggga tgatcccggc cgagcccttg tacggcacgt gcgtgacctt
    20101 gatcccggtc tcggcgcgca gcagctcgac ggcgaagtgg tgcggcgcgc cgacgcccgc
    20161 ggtgccgtag gtgagcggcc gcggcgacgc cttggcgtaa tcgatgaact cgcgcagcgt
    20221 gtgcaccggc gtcttcgagt tcacggtgag cccgaagggg atcgttgcga tcagcgcaat
    20281 cggctggaag tcgttgatcg ggtcgtacgg cagcttcgcg cccaccgccg cgctgatgac
    20341 gtggctcgcc tcggtcatga gcagcgtata gccgtcggcg ggcgccttgg cgacgaagtc
    20401 gcgcgcgatg tggcccgaag ccccgggctt gttctcgacc acgagcggct ggccgagcac
    20461 gggctgcagc ttctccgaga gcagccgcgc catgtaatcg ggcgagccgc ccggcggcag
    20521 cggcacgatg atgcgcatcg gtcgcgaagg ccaggcttgc gcggcggcct gtcctacgac
    20581 cgtgccgaga gccgccgcga gagcgacgac gaccagggag cggcgcgtca gcatggcgtt
    20641 cctccctatt tacgcgaggt cgcgctcgag ctcttcagct cggtgtcgta gcccggctgc
    20701 acgcgcacgt cgatcacgca caccgccccc gcgcgcaccg cgtcgatccc cttgcggatc
    20761 gcgggcagca ggtccttcgt ctcggtcacc gggccgatgc cttgcgcgcc ctgcgcgcgc
    20821 gccatcgcgg cgaggtcgat gtcgggatcg ccgatgcgct gcccgatcca gcggttctga
    20881 accggccgcg agcgctcgat cgcgacccgc tcctggtgca gctcgtcgtt gaagaacgag
    20941 cggttgttcg cgacgataat caggcacggg atgccgtagt gcgccgcggt ccacagcccg
    21001 gtgacgccca tgaggaagtc gccgtcgccg gtgatcgcga ccggcagccg cccgctgcct
    21061 ttgagcgcga gcgccgcgcc gacggtgttg ccgacgctcg cgccgacgcc gccgccgccc
    21121 tcgctgccga ggaagtccat cgggtggcgg aaatggcggt aggcgccgtt ccagccgagc
    21181 ggcacgcgcg tgatcgagac gtcgaggcct tcggtggcgg cgttgaacgc gtcggcgagc
    21241 gtgcgcagcg acacgacttt cgcgtctgcc ggcggcagct cgggcttggg cgcgggcgcg
    21301 gcggcggggc gcgccttcac cgcgtcgagc agcagcggca ccgccgcgtc gggctcgcac
    21361 atcaggtaga cgtccgcggg cggcaggccc tggtagtcca tgctccagcc gcggtggatg
    21421 tgcgcgtcgg gcgagacgtt gatgaccttg gcgccgacgg cctcggcacc ccaggcggcc
    21481 ttcagcgtgc cggcgaggtc gaccgcgtcg agcgagagga cgacgtccgc ctcgcggatc
    21541 attttcgcga cgaccggcga atggaacgcc gagggcgggg cggcgtgcag cgggtgatcg
    21601 gtcggaaacg ccgccgcgat cttgatgtcg gtgaagacgc gcgcctgcag cttctccgcg
    21661 agcgcgacgc gctgcttcca gcccgattcg ctgcgcgaga cgcggccggc gaggatcacg
    21721 ggatgcttcg cctgcgacag cagcttcgcc gcctgctcga ccaggtgcgg cgccgggatc
    21781 acgggatcgg gcgcgcggaa ccgggccggg tcgggcacgg gcggcagcgc gccgatcttc
    21841 tgctcctgca gcgcggcgtc gagattgatg taggtcggcc cacgcggcgc ggtgttcgcg
    21901 atctgcgcgg cgcgcagcag cgcctcgtac gccgcgagcg cggagccggg ctggttgtcc
    21961 cacttggtga agccgcgcac gagcgcgccc tggtcggtcg cggtgtggat ccagtcgatc
    22021 cacgggcggc gcttcgccgc gtcccacggg cccgtcgcgc cgagcacgag catcggcacg
    22081 cggtcgcacc aggcgttgaa gatcgccatg gtcgcgtgca tcaggccgac gttcgagtgc
    22141 acgaccgcgc ccatcatctt gcccgaggcc ttggtatagc cctgggcgat gtggatcgcc
    22201 gcctcctcgt gcaggcacag cagcatctgc ggggcgctgt tgccgagata gttgacgatc
    22261 gagtcgtgca ggccgcggta gctcgcgccc gggttgagcg ccaggtaggg aatgccgagg
    22321 gcgcgcagca tcgcggcgac ggcgtcggag ccccagaact ccttcggctc ggcgcccgcg
    22381 accggcgtgt cgtggcggac tgcgttggcg gcgaggttct ctttcgcgtt catggggact
    22441 ccgtcgggtg atctgtggct ggaagcgctg cgattctcgt gtacgaaggc ttgatcggtc
    22501 aatctaggcg agcgcgccga ccgcggcaaa gagcgacgcc accgcgtcgc gctcgacgcc
    22561 ccgggtaatg aagaccaggc gcgagcgccg ctcctcgctc gaccaggact ccagcgtcac
    22621 cggcgggtgg aacacgtgct gcgccccttg caccaccacg gggccgcgct cgccctggac
    22681 gttgatcagc cctttcaccc ggagcaggtc gggcccgcgc agcgacgtga gcgcctgcac
    22741 cgcggcatgg aacgagtccc aggtgaaggg cttgtcgaac cagaggacga atgagcggat
    22801 cgacggatcg tgcacggcgc gcaccgcttt tccgaggtaa gcgccctggc cggtgacatc
    22861 gggagagccg gacggagcga gccagcggct cacctcgtcg acgttcgcgc gggcgctccg
    22921 gggcccgatc gagcgcagca gctccggatc cagctcgccg ttgatcgctt ccgtgcgcgc
    22981 cgcgtacgga ttcatcgcct cgagccggtc gtggagcgcg gcgaccgtcg ccggcggcgc
    23041 gaggtcggtc ttggtgacga cgatgcggtc ggcgaccgcc acctggcgcg gcgcctcggg
    23101 cgcgcggtcg agcgtcgcgg cgccgttcac cgcgtcgacg agcgtgacca cgccgtcgag
    23161 ccggtactgc gcgccgagca gcccgtcgct ctgcagcgtg tgcagcaccg gcaccgggtc
    23221 ggcgagcccg gtggtctcga cgaagacgcg gtcgaagtcg atcacctcgc ccgcgcggcg
    23281 gcggatgaac agctcgcgca gcgtctcctg gaggtcggtg cgcacggcgc agcacaggca
    23341 tccgttctcg agcagcgtca tcgactcgga cgactgcgcg acgagctcgt ggtcgatgcc
    23401 gacctcgccc agctcgttga tgatcaccgc cgcccggttc atcgccgggt cgcgcagcag
    23461 gcggcggagc aatgtggtct tgccgctgcc gaggaacccc gtgatcaggg tgacgggcag
    23521 tttgccggcg agcgggtcgc ggccggcgct cgccatcaga tgttcatctc caggatgtac
    23581 aggccgccga tgaagcggtc gaccgtatac accaggccgc ggtcgtcgac gtagacgtcg
    23641 ttcagctgcg ccgagcccac gggcgatccg ttcggagtgc cgggcacgta ataggcgacc
    23701 tcttgcggct gataggggtt gccgacgtcg aaggcgcgca cgccgcccga gaagaacgtg
    23761 ccgaccacga tctcctgcga gcgccacgag cccggcaccg gcaggttctc gtgcaggttg
    23821 tgcgcgccga agcgcccgcc gcgcttggtg aaggtctcgt gcggcggcag cggaaaggtg
    23881 ctgatcggca ccgggttctt ttcctcgcgc gcgtcgacca cccacacgag cttgggccag
    23941 tcggcgccgt cgttcttgat gcactcgtcc gagacgatga gcagcccgcg gtcgagcagc
    24001 ggcatcaccg tgtggcagaa gccgttgtaa ggcggcgagt agcgccagtt ggcgaccagc
    24061 ttcggccggg acttgtcggc gatgtcgagg atcaccgcgc cgccgtcgat gtagccgacg
    24121 tacgcgcggt cgggccgctc ggggtagacg ttggtgttgt gcgcgcggta gccggcgtcg
    24181 atcttcggca gccgcgcggg cggcggcacg tcgtcgccga cgcgcgtgcc gggaaaccac
    24241 cagcgcccga cctcgcgcgg gctcgacgtg ttcttcacgt cgacgattcg gtagcactgg
    24301 tcgtcgttcg ggtgggtcgg ctggaagtcg gccgcgcccg ccgccatgtg cacggtctcg
    24361 ccgtcgacga accacaccgc gtgcacgccg cgcgaatgcg ggcccgagca gtcgaagtga
    24421 gagatcacgc gcggcttctc gggatcggag atgtcgaaca gctcgaagcc ggcgggcttg
    24481 aggccgacct ccttggtctg gtaggcgacc gccatcacgt cgccgacgac gtcgagcgag
    24541 ttggagcgca ccttcgcgtg cggcagctcg gtctgcacca cgacgtgcgg cttgcgcggg
    24601 tcggtgacgt cgaccgcggt gaagttcttg ggcgcggact cgtgcgcgag ccacaggatg
    24661 cggcggccgc gggtgagctg catgcccatg ccttcgccga tgccgccgaa tccctgcagc
    24721 tcgtggtggg acagcaggcg catgttgagc gcgagcgtct ggtcggctcg cggtccgatt
    24781 cgggtcgagg gcatcgaaga ggtcctgtga gattggccgg cgcgccgcga tcgcggcgcg
    24841 aaggtgcaag gttagcggcc cggcgccgcg cgcgcaacgc ttcgggtatg atttcgcctc
    24901 cccgaggagg atatcgatgc accgactgat ccgcagcacc atcggttggg tcgcggcgtt
    24961 cgcgtgcggc gcggcgctgg ctcagacccc ggcctacccg aaccatcccg tgaagctgat
    25021 cgtgacctat ccgccgggcg ggagctccga cctgatggcg cgcgtgttcg gcgccaagct
    25081 cgccgagcac tggggccagc aggtgatcac cgagaacaag ccgggcgccg ccggctcgat
    25141 cggcatggag tatgcggcca ggcagcccgc cgacggctac agcttcgtcg tcggcaacat
    25201 cgggcccgcg gcggtgaacc cgctgctcac caaggtcccg tacagcatgg accgcgattt
    25261 cgtcgcgatc tcgatgctct cgacgggccc gaacatcctg atcgtgcatc ccgacgtgcc
    25321 ggcgaagtcg ctcgccgagc tgacgtcgta cgcgaaagcg aacgccggca agatgaacta
    25381 tggcaccagc ggcccgggca gcatctcgca gctcgtcggc gcgatgtaca agaacatcgc
    25441 cggcgtgagc gcgaccgaag tgccgtacaa ggggggcatc ctcgcggtgc aggacgtcgt
    25501 cgccgggcac ctgcagtaca tcttctccga tacgctgccc gcgatgcagt tcatcaagag
    25561 cggccgcgtg cgcccgctgt gcaccacggg cgcgaagcgc agcgacatcc tgcccgagta
    25621 cccggcgtgc gccgaggcga tcccggggct ggtcgcgatc aactggtggg gcgtgttcat
    25681 gcccgccggc acgcccaagg cgatcaccga caagttccac ggcgacctgg tgaaggtgct
    25741 tcaggacccc gacgtgaaga agaagttcgc cgatctcggc gtcgaggccg tgtcgagcac
    25801 gcccgaagag ctcgtgcgct tcatgaaatc cgagacggcg aagtactcca agctgatcca
    25861 ggaagccggg atcaaggcgg agtgacggtc tagcgccgtc tgcgcgtctt cgcgtaaagc
    25921 tcgaccggga cgagcgacat gtcgctgccg ccgcgacctt cgcgcacggc cgcgtcgtag
    25981 cactcgagcg cgctcgcgct gaccggcagc ttcacgccga gcgcttcggc ctcctcgatc
    26041 atcgcggcga ggtccttgcg cgcgagctcc acgtccgagg cgaccggtcc cggcgcgccg
    26101 cctttcatca ccgcggcgat cgccggtccg cggcctttca ccgcgttcgt gccccccgac
    26161 gtatcggcga aaatgtccat gagccgcgtc tgctcgatgt cgagcggccc gcacagcgcg
    26221 agcgcctccc cgagcgcctg ccagtacacg agcagcggca gattgatcgc gagcttcacc
    26281 gtggcgccgg cgcccaccgg cccgacgtgc tcgacgcggc gacagagctg ctcgagcagc
    26341 ggccgtgcgc gcgcgacgtc ctcgggctcg ccacccacga agccgaagag ctttccctcg
    26401 cgcgccgggc cgaccgtgcc gccgacggga cactcgacga acgccgcacc cgcggcgcgc
    26461 accttcggcg cgagggcacg ctgcacgtcg ggccgcacgg tgctcatctc gatgaacagc
    26521 ttgccggcgg gctcgccgtg cagcagcccc tgcaggcctt cgtacgcgct cgcgatcgcc
    26581 tgcgcgtcgg agacgatcga gatcacggct tcggagcgcg tcgcgacggc cgcgggcgtg
    26641 gccgccgacg tcgcgcccgc agcgaccagc ggccgcgtct tgtcgggcgt gcggttccag
    26701 acggtcacct cgtgcccgag acccagcagc cgcgtcgtga tggccgagcc catcctgccg
    26761 atgccgacta cgccgatttt catgccgcct ccagcgctcc ggcgcccggt tgacatccct
    26821 gggcggccta gagtgaaatg aaggcgccaa tcatagcaac ttcaccccct cgtcagcctt
    26881 acaccatgga ggagaggcga tgagttcgcc gaaaagcgag ctccgcgacg gcatgcgcat
    26941 cgactgggac gtcgcgatcc gcatggacga cggcctcgag ctgcgcgccg acgtcttccg
    27001 cccggacgac ggcgcg
//
LOCUS       DATDMW010000100         4806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2268669, whole genome shotgun sequence.
ACCESSION   DATDMW010000100 DATDMW010000000
VERSION     DATDMW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2268669"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VI319_04385"
     CDS             <1..615
                     /locus_tag="VI319_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY6863116.1"
                     /translation="EASHLEGAKWLAQGTIYPDVIESAGAKTKKAQNIKSHHNVGGLP
                     ETLHLKLLEPLRELFKDEVRELGVALGLPREMVFRHPFPGPGLGVRILGAIERPYVDL
                     LQRADAIFIDELRAAGWYDQVAQAFAVFLPVRSVGVMGDGRTYEHVVALRAVQTTDFM
                     TAHWAPLPHDLLAKVSNRIINEVRGINRVVYDVSSKPPATIEWE"
     gene            606..1025
                     /gene="tadA"
                     /locus_tag="VI319_04390"
     CDS             606..1025
                     /gene="tadA"
                     /locus_tag="VI319_04390"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY6863117.1"
                     /translation="MGIEPDERFMREALELAKLAAERGEVPVGAVVVRDGEIIGRGYN
                     APISGHDPTAHAEIAALRDAARATGNYRLPGTDLYVTLEPCDMCVGAMFHARIARVVY
                     GAKDPKKLVLKNQVAIEGGVLAEEAGRLLSDFFAARR"
     gene            1022..1501
                     /locus_tag="VI319_04395"
     CDS             1022..1501
                     /locus_tag="VI319_04395"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY6863118.1"
                     /translation="MRIEVSIPAQTLALLDDAGRELARWRVSTAKNGAGEVRDSLCTP
                     RGRHVVRAKIGAGAAPNTVFVRRRPTGEIWTPLLAREFPGRDWILTRILWLSGCEPGF
                     NRLGDVDTMRRYIYIHGSPDAVPMGIPGSIGCIRMRNADIVELFDRVPARTPVEIRG"
     gene            complement(1498..1995)
                     /locus_tag="VI319_04400"
     CDS             complement(1498..1995)
                     /locus_tag="VI319_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY6863119.1"
                     /translation="MRTPATIAKHPLHPMLIVFPVGLWIFSLVCDIVVLAGATSPGWQ
                     TTAFYTMVGGLVGALVAAVPGLIDLVSPQLARVRRIAVTHMVLNLVAVALYAVNIWLR
                     TQPTADMRYPVLLSVVAVVILAVSGWLGGEMVHVHGVAVEDGALPAPHGGRSRSERLD
                     AAPQR"
     gene            2287..2841
                     /locus_tag="VI319_04405"
     CDS             2287..2841
                     /locus_tag="VI319_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6863120.1"
                     /translation="MLTLAAGVALPAAAQTTRETTVTTERYYMPYEKNFWSYIGANVG
                     QSKLKDSDFGISDNTDTGFKIYGGGKFRDWLGLELAYMNFGKAKLTNGGEEKAQGLNV
                     QAVVGLPLGANSSIFAKGGAAYTRADVIGAKVNGWEPSYGAGAAIGLNRNWQIRVDWD
                     RVRFEVPNGKDNFEMVSAGLQYRY"
     gene            2863..3135
                     /locus_tag="VI319_04410"
     CDS             2863..3135
                     /locus_tag="VI319_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863121.1"
                     /translation="MAFGFLGRRRRGPRRDRGAVVASPGARMPHERDQVVQQPPRPKD
                     ELRRAARDEESGRQDTEARWRATDEFSKNARPEKPGPVIIRRRYRI"
     gene            3359..4342
                     /gene="galU"
                     /locus_tag="VI319_04415"
     CDS             3359..4342
                     /gene="galU"
                     /locus_tag="VI319_04415"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY6863122.1"
                     /translation="MQTRRQTVRKAVFPVAGFGTRFLPATKACPKEMLPVVDKPLIQY
                     AVEEAAAAGITEMIFVTGRNKRAIEDHFDRAYELEKELEAAGKTDTLRELRNALPSGL
                     TYSYVRQPEMRGLGDAVLCAASLVGDEPFAVILPDDVIDAEIPAMRQMVELFEQTRSS
                     VVAVERVDPAETHRYGVIKPESAAGGPVQRLADLVEKPKPGTAPSSLAIVGRYILSPA
                     IFELLRMLQARVEKGELQLTDGIRGLLGLESVLAYEFEGVRYDCGTKIGHLQAQVWYA
                     LKRPELRDQFASYLRQLSWRPRQKAPAVHRDEPSRAWSKSNNPLRVVSERD"
     gene            complement(4339..>4806)
                     /locus_tag="VI319_04420"
     CDS             complement(4339..>4806)
                     /locus_tag="VI319_04420"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY6863123.1"
                     /translation="RPYLQGARLTAWEMVQEKIPVTLITDSMAGHMMRRDEVDLVIVG
                     ADRVAANGDAANKIGTYPLAVLAARHGLPFYVAAPVSTIDLEVASGDAIPIEERSASE
                     VTGYGGLRWAPEGVRVANPAFDVTPAELVTGLITERGVVERPDRAKIERLLGR"
BASE COUNT          830 a         1624 c         1664 g          688 t
ORIGIN      
        1 gaggcctcgc acctcgaagg cgcgaaatgg ctcgcgcagg gcaccatcta tcccgacgtc
       61 atcgagtcgg ccggcgccaa gaccaagaag gcgcagaaca tcaagtcgca ccacaacgta
      121 ggcggcttgc cggaaacgct gcatctcaag ctgctggaac cgctgcgcga gctcttcaag
      181 gacgaggtgc gagagctcgg cgtcgcgctc ggcctgccgc gcgagatggt gttccgccat
      241 ccgtttcccg gcccgggcct cggcgtgcgc atcctcggcg cgatcgagcg gccgtacgtc
      301 gacctcctgc agcgcgccga cgccatcttc atcgacgagc tgcgcgccgc cggctggtac
      361 gaccaggtcg cgcaggcctt cgccgtgttc cttcccgtgc gctccgtcgg cgtgatggga
      421 gacggccgga cctacgagca cgtcgtcgcg ctcagggcgg tgcagaccac cgacttcatg
      481 accgcgcact gggcgccgct cccgcacgat ctgctggcga aggtctcgaa ccgcatcatc
      541 aacgaggtgc gcgggatcaa ccgggtcgtc tacgacgtct cgagcaagcc gcccgcgacc
      601 atcgagtggg aatagagccg gacgagcgct tcatgcgcga ggcgctggag ctcgcgaagc
      661 tcgccgccga gcgcggcgag gttccggtgg gcgccgtggt ggtccgcgac ggcgagatca
      721 tcggccgagg ctacaacgcc ccgatctccg gccacgatcc cacggcacat gccgagatcg
      781 cggcgctgcg cgacgcggcg cgtgcgaccg gcaactaccg gctccccggc accgacctgt
      841 acgtcacgct cgagccttgc gacatgtgcg tcggcgcgat gttccacgcg cgcatcgcac
      901 gggtcgtgta cggcgcgaag gacccgaaga agctcgtgct caagaaccag gtcgcgatcg
      961 aaggcggggt gctagccgag gaggcaggcc gcctgctctc ggatttcttc gcggcgcgcc
     1021 ggtgagaatc gaggtctcga tcccggcgca gacgctcgcg ctcctcgacg acgcgggtcg
     1081 cgagctcgcg cgctggcgcg tctcgacggc gaagaacggg gcgggcgagg tccgcgacag
     1141 cctgtgcacg ccgcgcgggc gccacgtcgt gcgcgccaag atcggcgccg gcgccgcgcc
     1201 gaacacggtg ttcgtgcgcc gccgtccgac gggcgagatc tggacgccgc tgctcgctcg
     1261 agaattcccg ggccgcgact ggatcctgac gcggatcctg tggctctcgg gctgcgagcc
     1321 tggcttcaac cggctcggcg acgtcgacac gatgcgccgc tacatctaca tccacggcag
     1381 cccggacgcg gtcccgatgg gcatcccggg ctcgatcggc tgcatccgca tgcgcaacgc
     1441 cgacatcgtc gagctgttcg accgggtgcc ggcgcgcacg ccggtcgaga tccgcggcta
     1501 gcgctgcggg gcggcgtcga ggcgctccga gcgcgagcgc ccgccgtgag gagcgggcag
     1561 agcgccgtcc tcgaccgcga cgccgtgcac gtggaccatc tcgcccccga gccagcccga
     1621 caccgcgagg atgacgaccg cgaccaccga cagcagcacc gggtagcgca tgtcggccgt
     1681 cggctgcgtg cgcagccaga tgttgaccgc gtagagcgcg accgcgacca ggttgaggac
     1741 catgtgcgtg accgcgatcc ggcgcacgcg cgcgagctgc ggcgacacca ggtcgatcag
     1801 cccgggcacg gccgcgacga gcgcaccgac caggccgccc accatcgtgt agaaggcggt
     1861 ggtctgccat ccgggcgagg tcgctccggc gagcacgacg atgtcgcata cgagagagaa
     1921 gatccatagg ccgacaggaa acacgatcag catcggatgc agcggatgct tcgcgatcgt
     1981 tgcgggtgtt cgcatgatta ttcgctccat cgaggagagt tgacgcggag gagggtgcat
     2041 catccatgcc aatgcacgtg ctccgtttgc cggaaaaatt ttccgatgcg cgggcaatag
     2101 ttaccgcttc gcatgatccg aaagagggag gaaaacgcgg ctgcggccgg cttttgcgca
     2161 cggcatggag cttgctgccg cattgcgcga ggccgctttt ctgcgcccgg cggggtcgcc
     2221 ggggagcggc gattcacccg aggaacctga accatgcggc aatcgactct tctcgccgga
     2281 ctctccgtgc tcacacttgc agcgggcgtc gccttacccg ctgccgcgca gaccacgcgc
     2341 gagaccacag tcacgaccga acgctactac atgccttacg agaagaactt ctggagctat
     2401 atcggcgcga acgtcgggca atcgaagctc aaggactccg acttcgggat ctccgacaac
     2461 accgataccg gcttcaagat ctacggaggc ggcaagttcc gcgactggct cgggctcgag
     2521 ctcgcataca tgaacttcgg caaggcgaag ctcaccaacg gcggcgagga gaaggcgcaa
     2581 ggactcaacg tgcaggccgt ggtcggcctc ccgctcggcg cgaactcgag catcttcgcg
     2641 aagggcggcg ccgcgtacac gcgggcagac gtgatcggcg cgaaggtcaa cggctgggag
     2701 cccagctacg gggcgggcgc ggcgatcggc ctgaaccgca actggcagat ccgggtcgac
     2761 tgggaccgcg tccgcttcga ggtcccgaac ggcaaggaca acttcgagat ggtgtcggcg
     2821 ggcctgcagt accgctattg agaacgcaac aaggagaagc acatggcatt cgggttcctg
     2881 ggccgcaggc ggcgcgggcc gcggcgcgat cgtggcgcag tcgtcgcgtc cccgggggcc
     2941 cgcatgccgc acgagcgcga tcaggtcgtg cagcagccgc cgcgccccaa ggacgagctg
     3001 cgccgggcgg ctcgggacga ggagagcggc cggcaggaca cggaggcgcg gtggcgcgcg
     3061 accgacgagt tctccaagaa cgcacggccg gaaaagcccg ggccggtgat catccggagg
     3121 cgttaccgga tctagcggca acctttacaa ccgccctgaa aaatttaccg atgcgcaacg
     3181 atcgcgggcg cgaaagatcg aatgttttca ggccggcggc ttcccgtgcc ggacggcacg
     3241 gttcgtgctg cgtcaaacag acacacagcg tcgtctcatc cgaggcgaca gcagggacgg
     3301 caaggggatc caagcctgaa atttttcgtg gccaaggccg ctgattagga aattccatat
     3361 gcagacgaga cgacagacgg tccgcaaggc cgtatttccg gtggccggat tcggcacccg
     3421 gttcctgccc gccaccaagg cgtgcccgaa agagatgctg ccggtggtag acaagccgtt
     3481 gatccagtac gcggtcgagg aagccgccgc ggccgggatc accgagatga tcttcgtcac
     3541 cggccgcaac aagcgtgcga tcgaggacca tttcgaccgc gcctacgagc tcgagaagga
     3601 gctcgaagcc gccggcaaga ccgacacgct gcgcgagctg cgcaacgcgt tgccctcggg
     3661 cctcacctac agctacgtgc gccagcccga gatgcgcggc ctgggcgacg cggtgctgtg
     3721 cgcagcatcg ctggtcggcg acgagccgtt cgccgtcatc ctgcccgacg acgtcatcga
     3781 cgccgagatc ccggccatgc ggcagatggt cgagctgttc gagcagaccc gcagctcggt
     3841 ggtcgcggtc gagcgcgtcg accccgccga gacgcaccgc tacggcgtga tcaagcccga
     3901 gagcgcggcc ggcgggccgg tccagcgtct ggcggacctg gtggagaagc ccaagcccgg
     3961 gacggcgccg tcgtcgctcg cgatcgtggg ccgctacatc ctgagcccgg cgatattcga
     4021 gctgctgcgg atgctgcagg cacgcgtcga gaaaggcgaa ttgcagttga ccgacggtat
     4081 ccgcgggctg ctggggctcg aatcggtgct cgcctacgag ttcgagggcg tgcgttatga
     4141 ctgcggcacc aagatcgggc acctgcaggc gcaggtctgg tacgccctga agcggcccga
     4201 gctgcgcgac cagttcgcgt cgtacctgcg ccagctcagc tggaggccgc gccagaaggc
     4261 gcccgcggtg caccgcgacg agccgtcgcg cgcctggagc aagagcaaca atccgctgcg
     4321 cgtggtgagc gagcgcgact agcgcccgag gagtcgctcg atcttcgccc ggtcggggcg
     4381 ctcgacgacg ccgcgctcgg tgatcagccc ggtcacgagc tccgccggcg tgacgtcgaa
     4441 cgccgggttc gcgacgcgaa cgccttcggg cgcccagcgc agcccgccgt agccggtgac
     4501 ctcggacgcg ctgcgctcct cgatcgggat cgcgtcgccg ctcgcgactt ccaagtcgat
     4561 ggtcgagacc ggcgccgcga cgtagaaggg caggccgtgc cgggcggcca gcaccgcgag
     4621 cgggtacgtc ccgatcttgt tcgccgcgtc gccgttggcc gcgacacgat cggctccgac
     4681 gatgaccagg tcgacctcgt cgcgtcgcat catgtgcccg gccatgctgt ccgtgatcag
     4741 cgtgacggga atcttctcct gcaccatctc ccaggcggtc agccgcgcgc cctgcaggta
     4801 cgggcg
//
LOCUS       DATDMW010000101        11898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1147899, whole genome shotgun sequence.
ACCESSION   DATDMW010000101 DATDMW010000000
VERSION     DATDMW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147899"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..491
                     /gene="rimI"
                     /locus_tag="VI319_04425"
     CDS             69..491
                     /gene="rimI"
                     /locus_tag="VI319_04425"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY6863124.1"
                     /translation="MTEADLESVLRVERAIYDFPWTRGNFRDSLRAGYSCWIARSEER
                     LAGYAVMMLAAGEAHLLNLSVAAERQRRGHGRALLAHLLDVAREHRARVLFLEVRPSN
                     AAAQRLYASFAFEQIGVRRGYYPAHGGREDALVYSINL"
     gene            488..1342
                     /gene="ung"
                     /locus_tag="VI319_04430"
     CDS             488..1342
                     /gene="ung"
                     /locus_tag="VI319_04430"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY6863125.1"
                     /translation="MSLRERRLAEMGLGPVWKLRSRATTVRPEPVEGPAEGPVEAPVE
                     KTAAPAAGDDAGSKIKLHPSWKSRLIAEFNKPYMADLREFLRKEKAAGKTIYPKGNEY
                     FAALDHTPFDGVKVVILGQDPYHGPSQAHGLCFSVRPGVALPPSLQNIFAELKNDLGI
                     EPPGHGHLTAWAEQGVLLLNATLTVEAGKAGSHQNRGWEQFTDAVIHALAREREHLAF
                     VLWGSYAQKKGAFIDSRRHLVLKSPHPSPLSAHNGFFGCRHFSKINDYLRQHGQAPID
                     WRLPPLSS"
     gene            complement(1370..1837)
                     /locus_tag="VI319_04435"
     CDS             complement(1370..1837)
                     /locus_tag="VI319_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY6863126.1"
                     /translation="MAKSTFVYVTYIRTTPEKLWAALTGPEFQEQYWFGCHCESGWTA
                     GSPWKLVYPDGRLADSGEIVEAEAPRRLVIRWRNEWKPELKAEGWSLCTMEIEPGPGA
                     VKLSITHSVERDASKFIEAVSGGWPKVVSNLKSLLETGTAALLEPYPAAAAAR"
     gene            complement(1830..2153)
                     /locus_tag="VI319_04440"
     CDS             complement(1830..2153)
                     /locus_tag="VI319_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY6863127.1"
                     /translation="MADTDLLFKALADPSRRKLLDLLHAHDGRTLGDLCEHLDMSRQA
                     VTQHLDVLEAASLVAPLRRGREKLHFLNPVPLQEIYERWIAKFEKPRLKALTDLKRRL
                     EKAHG"
     gene            2276..2887
                     /locus_tag="VI319_04445"
     CDS             2276..2887
                     /locus_tag="VI319_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY6863128.1"
                     /translation="MRIFQGAVAVLVVLLLSGCGYNDIQRGDEQVKAAWSEVLNQYQR
                     RNDLVPNLVNTVKGFAAQEQNVLTQVTEARARVGSIQATPELVNDPQAFRRFQDAQAS
                     MTSALSRLLVVAENYPQLKSDQNFRDLQAQLEGTENRIAVARNRYIRSVQEYNTMIRQ
                     FPVNLTAMVFHYEPKPNFSVEDEKAISRPPAVDFGAPKAAPAK"
     gene            2884..3753
                     /locus_tag="VI319_04450"
     CDS             2884..3753
                     /locus_tag="VI319_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY6863129.1"
                     /translation="MKHLAAFVLALAALLAAAAFAQAPVPPYKARVVDLTGTLTAAQA
                     QALESALRELEQRKGSQIAILMVPTVQPESIEQYSIRVAEAWKSGRRNVDDGALIVVA
                     KNDRQARIEVGRGLEGAIPDVTASRIGREIMAPHFRDGDYYGGLSEATRALVKLIDGE
                     PLPPPPAPGQDYGHLLVPLLLVALFGGAFLSRLLGAGGGALGTGALVGFIAWALTGLL
                     LAAVIAGLIGFVLGLFGAASGARHRGGWGGPWGGGGWGGGGWGGGGGFGGGGGFGGGG
                     GGTFGGGGASVRW"
     gene            3756..4256
                     /locus_tag="VI319_04455"
     CDS             3756..4256
                     /locus_tag="VI319_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY6863130.1"
                     /translation="MDLGRALRHLFTPHRAVARVFPDASMQAIERGIAEEEKRHAGEI
                     QFAVESALPLIELLRGTSARRRAIGWFARLHVWDTAHNSGVLIYLLLADRRIEIVADR
                     GISAKVDGRTWELICGEMQREFAKGRFQQGVVLGIRAIGDVLAAHFPARGGPNPNELP
                     DKPVVL"
     gene            complement(4253..5548)
                     /gene="lplT"
                     /locus_tag="VI319_04460"
     CDS             complement(4253..5548)
                     /gene="lplT"
                     /locus_tag="VI319_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEY6863131.1"
                     /translation="MKRGFYTIMAAQFFSSLADNALLIAAIAMLLELQGPPWMTPLLK
                     FFFTVSYVALAAFVGAFADALPKGRVMFITNTVKIVGCAMMFLPGWVQVGSLPDYAMV
                     LVAYGVVGLGAAAYSPAKYGILTELLPPSQLVVANGWIEGLTVTSIIVGTVLGGALIS
                     PKVSAALLSLDVPNIDTGIDTPAEAAITVIAGFYVVAALFNLFIPDTGARYARQRAHP
                     GYLIRDFWICFATLWKDKLGQVTLGVTTLFWGAGATLQFIVLKWAEVHLGLPLDQGAV
                     LQGVTAIGIAAGAVIAGRFVPLRRSLSVLPVGIAMGLTMLTMLGAHNMVLVYFLLILI
                     GAMAGFFVVPMNALLQHRGHVLLSAGHSIAVQNFTENISILVMLAIYAAMIKADVHIN
                     KVIVLFGTFVSCAMLLVMLLHRYNQRRGDSLHLIGMEKR"
     gene            5737..6639
                     /locus_tag="VI319_04465"
     CDS             5737..6639
                     /locus_tag="VI319_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6863132.1"
                     /translation="MADRRLQVFHAVAKQMSFTKAAETLFMTQPAVTFQIKQLEEQYN
                     TRLFERGHGRVALTPAGEIVYDYAERILALSAELDTRLKEMTGRLAGPLNIGASSTIA
                     EFMLPRVLGEFKSRYPDVHARLFVANSGIVEGRVGEHSIDVGLIEAPSHLPALTTEPC
                     FEDELQVVVSPSHPLAKLKSVTPKQLLAHPYVSREEGSGTREFTEIYMRKAGIGESEL
                     RIEMELGSPEALKGVVCAGAGFAILSELAIEKEKKLGDLVAVPLQPRLMRMLSLVYPK
                     EKFRSRLVATFVEFAKQKLRPPGH"
     gene            6647..7708
                     /gene="alr"
                     /locus_tag="VI319_04470"
     CDS             6647..7708
                     /gene="alr"
                     /locus_tag="VI319_04470"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY6863133.1"
                     /translation="MRPTAARIHLSAFRHNLAVVRRHAPKARVWAVVKADAYGHGLKR
                     AARALDAEGLALLELEGALALRSAGERRPMLLLEGFFSPRELPSFAEHDLTPVVHSLE
                     QVAMLEKARLDSRLGVYLKLNTGMNRLGLNPAEFRGALARLQECRSVAGVTLMTHFAD
                     TEGGTRMRDQCVIFDAASAGSRLPRSLANSAAILRCPEAHGDWVRPGIMLYGCSPFAD
                     ASAESLGLKPAMTLVSEIIAVRDLAAGDSVGYGWTFTAPAPMRIGVVACGYADGYPRH
                     APTGTPIVVAGKRTRTVGRVSMDMLFVDLGGIANASVGSNVILWGEHVSADEVAAAAG
                     TVSYELLCGLGGRVPVSEI"
     gene            7734..9089
                     /gene="radA"
                     /locus_tag="VI319_04475"
     CDS             7734..9089
                     /gene="radA"
                     /locus_tag="VI319_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY6863134.1"
                     /translation="MAKAKTVYVCTECGGQSPKWQGQCPACAAWNTLVEAAAEAPARH
                     RFAGVARASGLQRLADVAAKESHRTPTGIEELDRALGGGLVAGQVVLIGGDPGIGKST
                     LLLQALARLSAETRALYVSGEESAEQVALRAQRLQLDCGKLEVLAEIQLERILEALES
                     AKPKIAVVDSIQTLYSEALTSAPGSVAQVRECVAQLARWAKQSGTVIIQVGHVTKEGA
                     LAGPRVVEHIVDTVLYFEGDTQQSFRVVRAFKNRFGAVNELGVFAMTERGLKGVSNPS
                     ALCLSGHSGEAAGSCIMVTQEGTRPLLVEIQALVDAAQAPNPRRLGVGIEQNRLAMLL
                     AVLHRHAGISCGDQDVFVNAVGGVRIAEPAADLAILLAVVSSLADKPLAPKLVVFGEV
                     GLAGEVRPAPRGQERLKEAAKLGFAQAIVPRANQPRQPIEGLEVIAVDRLADAIVRFR
                     S"
     gene            9334..11301
                     /locus_tag="VI319_04480"
     CDS             9334..11301
                     /locus_tag="VI319_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6863135.1"
                     /translation="MITPESAPRTDDERLTAARAAAHGPLPQELEDPFRDLVAAVEEY
                     AIFLLNAKGEIQSWNAGAERIKGYEAHEILGRHFSVFYPPESVASGWPDHELRVAEAA
                     GRFNDEGWRLRKDGNRFWASVTITALRHRDGALRGFLKITRDLTERKRAEEGLRQSEE
                     RFRLLIEGVQDYAIYMLDPNGNVASWNVGAERIEGYRAEEIIGRHFSAFYPPEAVAQG
                     RPDWQLRTALEQGRAEDEGWRTRKDGTQYWANAVITALKDKDGTLRGFAKIVRDMTER
                     RKIEALQRADRQKNEFLALLAHELRNPLAPIRNALYIMGQPEATSANATRAREIAERQ
                     VQHMSRLLDDLIDVARVSRGTMELRKELIDVDVAVASAVEAVRPLVQDQRHQLTVAVP
                     PRTLWVDADPARLEQVLINLLTNAIKYTDPGGHIWITAETQGEEVVLRVRDSGIGVDP
                     VMLPRIFELFVQAERRMDRAVGGVGIGLSLVKKLIELHGGTVQAFSAGLGKGTELVVR
                     LPAAKTPEAGGAQSRTDGVPSDLFGLRILVVDDNINAADSLATLLELAGHEVRVAYEG
                     EAALLVAEAFKPQVVLLDIGMPGMDGYEVGRALRQKPQTRSALLVAITGWGAPDDLRR
                     SKEAGFDHHLVKPVEPAALQRLLARRSIRTR"
     gene            complement(11323..>11898)
                     /locus_tag="VI319_04485"
     CDS             complement(11323..>11898)
                     /locus_tag="VI319_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6863136.1"
                     /translation="GEPGRFRLDDEADSYSHVVVACAPQHVAALLPKWSGLEGVRAAL
                     GRFEYEPIVTCYLQYPDSVALSAPMIGFAGGLIQWVFDRGRLGGPPGLLAAVISGSGR
                     HAEMESPALTAAVEREIEAAWGRLPAPLWSRTIAERRATFSCAPALERPATVTPVPGL
                     LLAGDYVASDYPGTLETAVRSGLAAARAITP"
BASE COUNT         1840 a         4144 c         4228 g         1686 t
ORIGIN      
        1 aagacgagcg agcgcgcatg agcgcagtgc ccaggcccgc gtcgggaccc gcgacgcatt
       61 acgcgccgat gaccgaggcg gacctcgagt ccgttctccg agtcgagcgg gcgatctacg
      121 acttcccctg gacgcgcggc aacttccgcg actcgctgcg cgcaggctac agctgctgga
      181 tcgcgcgcag cgaggagcgg ctcgcgggct acgcggtgat gatgctcgcg gcgggcgagg
      241 cgcacctgct gaacctctcg gtggccgcgg agcggcagcg ccggggtcac gggagggcgc
      301 tcctcgcgca cctgctcgac gtcgcgcgcg agcaccgcgc gcgcgtgctg ttcctcgagg
      361 tgcgcccgtc gaacgcggcg gcgcagcgcc tgtacgcgag cttcgccttc gagcagatcg
      421 gcgtgcggcg cggctactat ccggcgcacg gcgggcgcga ggacgcgctg gtctactcga
      481 tcaacctatg agcctgcgcg agcgacgtct cgccgagatg ggcctcggcc ccgtgtggaa
      541 gctgcgctcg cgcgcgacga ccgttcgccc tgagcctgtc gaagggcctg ccgaagggcc
      601 cgttgaagcg cccgtcgaga aaaccgcggc gcccgccgcg ggcgacgacg ccggctcgaa
      661 gatcaagctg cacccgtcgt ggaagtcgcg cctcatcgcc gagttcaaca agccgtacat
      721 ggccgacctg cgcgagttcc tgagaaagga aaaggccgcg ggaaaaacga tctacccgaa
      781 gggcaacgag tacttcgccg cgctcgacca cacgcccttc gacggcgtga aggtcgtgat
      841 cctcggccag gatccgtacc acggcccgag ccaggcgcac gggctgtgct tctcggtgcg
      901 tcccggcgtc gcgctgccgc cgagcctgca gaacatcttc gccgagctga agaacgatct
      961 cggcatcgag ccgccgggcc acgggcatct cacggcatgg gcggagcagg gcgtgctgct
     1021 gctcaacgcg acgctcacgg tcgaggccgg caaggcgggc agccaccaga accgcggctg
     1081 ggagcagttc accgacgccg tcatccacgc gctcgcgcgc gagcgcgagc acctcgcgtt
     1141 cgtgctctgg ggcagctacg cgcagaagaa gggcgcgttc atcgattcgc gccggcacct
     1201 ggtgctgaag agtccgcacc cgtcgccgct ctccgcgcac aacggcttct tcggctgccg
     1261 gcacttctcg aagatcaacg attacctgcg ccagcacggg caggcgccga tcgactggcg
     1321 cctgccgccg ttgtcgagtt gactggcgag ctcattcgcg accggggcgt cagcgggccg
     1381 cagcagccgc cggatagggc tcgagcagcg cggcggtgcc ggtctcgagc agagacttga
     1441 gattggacac gactttcggc cagccgcccg acaccgcctc gatgaacttg gaggcgtcgc
     1501 gctcgacgct gtgggtgatg gagagcttga ccgcgcccgg gcccggctcg atctccatcg
     1561 tgcacagcga ccagccctcg gccttgagct cgggcttcca ttcgttgcgc cagcggatca
     1621 ccaggcgccg cggcgcctcg gcctcgacga tctcgccgga gtcggcgagg cggccgtcgg
     1681 gatacaccag cttccacggc gatcccgccg tccacccgct ctcgcagtgg cagccgaacc
     1741 agtactgctc ctggaactcc gggccggtca gcgccgccca aagcttttcg ggcgtggtgc
     1801 ggatgtaggt cacgtagaca aaggtcgatt tagccatggg ctttctccag tcgtcgtttc
     1861 aggtcggtga gcgccttgag gcgcggcttt tcgaacttgg cgatccagcg ttcgtagatc
     1921 tcctgcagcg gaaccgggtt gaggaagtgc agcttttcgc ggccgcggcg caagggggcg
     1981 accaggctcg ccgcctccag cacgtcgagg tgctgggtca cggcctgccg gctcatgtcg
     2041 aggtgctcgc acagatcgcc gagggtgcgg ccgtcgtgcg cgtgcagcag gtcgaggagc
     2101 ttcctccggc tgggatcggc cagggccttg aacaggagat cggtgtcggc catcgcaata
     2161 ggcaagtatc cgcttgcctg aatgataggc aggtaaatac ctgcatgtca agcttcgact
     2221 tgtaatcggc gccggcgccc ccatctacgc gccggtaccc ccacaggaga cccacgtgcg
     2281 catattccag ggagcggtcg cggtcctcgt cgtgctgctg ctgtccggct gcggctacaa
     2341 cgacatccag cgcggcgacg agcaggtcaa ggccgcctgg tcggaggtcc tgaaccagta
     2401 ccagcggcgc aacgacctgg tgccgaatct cgtgaacacg gtcaagggct tcgcggcgca
     2461 ggagcagaac gtgctgacgc aggtgaccga ggcgcgcgcg cgcgtcggca gcatccaggc
     2521 gacgcccgag ctcgtcaacg acccgcaggc cttccggcgc ttccaggacg cgcaggcgag
     2581 catgaccagc gcgctctcgc gcctgctcgt cgtcgccgaa aactacccgc agctcaagtc
     2641 ggaccagaac ttccgcgacc tgcaggcgca gctcgagggc accgagaacc gcattgccgt
     2701 cgcgcgcaac cgctacatcc gctcggtgca ggagtacaac acgatgatcc ggcagttccc
     2761 ggtgaacctc acggcgatgg tcttccacta cgagcccaag ccgaacttca gcgtcgagga
     2821 cgagaaggcg atctcgcgtc cgccggcggt ggacttcggc gcgccgaagg cggctcccgc
     2881 caagtgaagc acctcgccgc attcgtcctc gcgctcgcgg cgctgctcgc ggcagccgct
     2941 tttgcccagg cgccggtccc gccgtacaag gcgcgcgtcg tcgatctgac cgggacgctg
     3001 accgcggcac aggcgcaggc gctcgagagc gcgctgcgcg agctcgagca gcgcaagggc
     3061 agccagatcg cgatcctcat ggtgccgacc gtccagcccg agtcgatcga gcaatactcg
     3121 attcgcgtcg ccgaggcgtg gaaatcgggg cgccgcaacg tcgacgacgg cgccctcatc
     3181 gtcgtcgcga agaacgaccg tcaggcgcgc atcgaggtcg gccgcggcct ggagggcgcg
     3241 atcccggatg tcaccgcgag ccggatcggc cgcgagatca tggcgccgca tttccgcgac
     3301 ggcgactatt acggcggcct gagcgaagcc acgcgcgcgc tggtgaagct gatcgacggc
     3361 gagccgctgc cgcctccgcc cgcgccgggg caggactacg ggcatctcct cgtgcccttg
     3421 cttctggtcg ccttgttcgg cggcgcgttc ctgtcgcggc tgctcggcgc cggcggcggc
     3481 gcgctcggga ccggcgcgct ggtcggcttc atcgcgtggg cgctgaccgg gctgcttctc
     3541 gcggcggtga tcgcgggact gatcggcttc gtattgggcc tgttcggggc ggcgagcggc
     3601 gcacgtcatc gcggcggatg gggcggcccc tgggggggcg gcggttgggg cggcggaggc
     3661 tggggaggcg gaggcggctt cggcggaggc ggcggtttcg gcggcggagg cggcggcacg
     3721 ttcggcgggg gcggcgcgtc ggtgcgctgg tagccatgga cctcgggcgc gcgcttcgtc
     3781 acctgttcac gccgcaccgc gcggtggcgc gcgtcttccc ggatgcgtcg atgcaggcga
     3841 tcgagcgcgg gatcgccgag gaggagaagc gccacgccgg cgagatccag ttcgcggtcg
     3901 agtccgcgct gccgctgatc gagcttctgc gcggcacgag cgcgcgccgc cgcgcgatcg
     3961 ggtggttcgc gcggctgcac gtctgggaca ccgcgcacaa cagcggcgtg ctgatctacc
     4021 tgctcctcgc cgaccgccgc atcgagatcg tcgccgaccg cggcatcagc gccaaggtcg
     4081 acggccgcac ctgggagctg atctgcggcg agatgcagcg cgagttcgcg aaggggcgct
     4141 tccagcaggg cgtggtgctc gggatccgcg ccatcggcga tgtcctggcc gcgcacttcc
     4201 ccgcgcgcgg cggcccgaac ccgaacgagc tgcccgacaa gcccgtcgtg ctctagcgct
     4261 tctccatccc gatcaggtgc agcgagtcgc cgcgccgctg gttgtagcgg tgcagcagca
     4321 tgacgagcag catcgcgcac gacacgaagg tgccgaacag gacgatcacc ttgttgatgt
     4381 gcacgtccgc cttgatcatc gccgcgtaga tcgcgagcat cacgaggatc gagatgttct
     4441 cggtgaagtt ctgcaccgcg atcgagtgtc ccgccgagag cagcacgtgg ccgcggtgct
     4501 gcagcagcgc gttcatcggc acgacgaaga atcccgccat ggcgccgatc aggatcagca
     4561 ggaagtagac gaggaccatg ttgtgcgcgc cgagcatggt cagcatggtg aggcccatcg
     4621 cgatgcccac cggcagcacc gagagcgacc ggcgcagcgg cacgaacctc cccgcgatca
     4681 ccgcgcccgc cgcgatgccg atcgcggtca cgccctggag cacggcgccc tggtcgagcg
     4741 gcaggccgag gtgcacctcg gcccacttga ggacgatgaa ctgcagcgtc gcgcccgcgc
     4801 cccagaacag ggtcgtcacc ccgagcgtga cctggccgag cttgtccttc cacaacgtgg
     4861 cgaagcagat ccagaaatcg cggatcaggt acccgggatg cgcgcgctgg cgcgcgtagc
     4921 gcgcgcccgt gtccgggatg aacaggttga acagcgccgc gaccacgtag aacccggcga
     4981 tcaccgtgat cgccgcctcg gccggcgtgt cgatgccggt gtcgatgttc gggacgtcga
     5041 gcgagagcag cgccgccgac accttggggc tgatcagcgc gccgccgagc accgtgccga
     5101 cgatgatcga cgtgaccgtg aggccctcga tccagccgtt cgctacgacg agctggctcg
     5161 gcggcagcag ctcggtcagg atgccgtact tcgcgggcga atacgcggcg gcgccgaggc
     5221 cgacgacgcc ataggcgacc agcaccatgg cgtagtcggg caggctgccg acctgcaccc
     5281 agccgggcag gaacatcatc gcgcagccga cgatcttcac cgtgttggtg atgaacatca
     5341 cgcgtccctt gggcagggcg tcggcgaagg cgccgacgaa ggccgcgagc gcgacgtacg
     5401 agacggtgaa gaagaacttc agcagcggcg tcatccacgg cggcccctgc agctccagca
     5461 gcatcgcgat cgcggcgatc agcagcgcgt tgtcggcgag cgagctgaag aactgcgccg
     5521 ccatgatcgt gtagaagccg cgcttcatgc gatggagccg cgtgtcatcc gggagatggc
     5581 ctctggaaaa ggaaggaccg ccttcgttct tataatgtgc cggcgtctta taccatgact
     5641 ccgttcggac tacgcgccgg cccggaagac ggataccgcg cgcaggctgg tccagttatc
     5701 cttcttatgg ttcaataagc cggtagtcgg tgcggtatgg cagacaggcg cttgcaggtg
     5761 ttccacgcgg tggccaagca gatgagcttc accaaggccg ccgagacgct attcatgacg
     5821 cagccggcgg tgaccttcca gatcaagcag ctcgaggagc agtacaacac ccgcctgttc
     5881 gagcgcgggc acggccgcgt cgcgctcacg cccgccggcg agatcgtgta cgactacgcc
     5941 gagcgcatcc tcgcgctctc ggccgagctc gacacgcggc tcaaggagat gaccgggcgt
     6001 ctcgcgggcc cgctcaacat cggcgcgagc agcaccatcg ccgagttcat gctgccgcgg
     6061 gtcctgggcg agttcaagtc gcgctatccg gacgtgcacg cgcgcctgtt cgtggcgaac
     6121 tcgggcatcg tcgagggccg ggtcggagag cacagtatcg acgtcggcct gatcgaggcg
     6181 ccgtcgcacc tgccggcgct caccaccgag ccgtgcttcg aggacgagct gcaggtcgtc
     6241 gtctcgccgt cgcacccgct cgccaagctc aagagcgtga cgcccaagca gctcctcgcg
     6301 cacccgtacg tcagccgcga ggagggctcg ggcacgcgcg agttcaccga gatctacatg
     6361 cgcaaggccg gcatcggcga gagcgagctg cgcatcgaga tggagctcgg cagccccgag
     6421 gcgctgaagg gcgtcgtgtg cgccggcgcg ggcttcgcga tcctctcgga gctcgcgatc
     6481 gagaaggaga agaagctcgg cgacctcgtc gccgtgccgc tccagccgcg cctcatgcgc
     6541 atgctgtcgc tcgtctatcc gaaggagaag ttccggtcgc ggctcgtcgc caccttcgtc
     6601 gagttcgcga agcagaagct gcgtccgccg ggccactaga gcgcgcatgc gaccgaccgc
     6661 ggcccgcatc cacctctcgg cgttccgcca caatctcgcc gtcgtccgcc gccacgcgcc
     6721 gaaggcgcgc gtctgggccg tggtcaaggc cgacgcctac ggccacggcc tgaagcgcgc
     6781 ggcgcgcgcg ctcgacgcgg aggggctggc gctgctcgag ctcgaagggg ctctcgccct
     6841 gcgctcggcg ggcgagcgca ggccgatgct gctgctcgag ggctttttct cgcccagaga
     6901 attgccgagc ttcgccgagc acgacctgac gccggtggtg cacagcctcg agcaggtcgc
     6961 gatgctcgag aaggcgcgcc tcgactcgcg cctcggcgtc tatctcaagc tcaacaccgg
     7021 gatgaaccgc ctcggcctga atcccgccga attccgcggc gcgctcgcgc gcctgcagga
     7081 gtgccgctcg gtcgccggcg tgacgctcat gacgcacttc gccgacaccg agggcggcac
     7141 gcgcatgcgc gaccagtgcg tcatcttcga cgccgcgagc gcgggcagcc gcctgccgcg
     7201 gagcctcgcc aactcggccg cgatcctgcg ctgccccgag gcgcacggcg actgggtgcg
     7261 gccggggatc atgctctacg gctgctcgcc gttcgccgac gcgagcgcgg aatcgctcgg
     7321 gctgaagccg gcgatgacgc tcgtctcgga gatcatcgcg gtgcgcgacc tggccgcggg
     7381 cgactcggtg ggctacggct ggaccttcac ggcgccggcg ccgatgcgca tcggcgtcgt
     7441 cgcctgcggc tacgccgacg gctatccgcg gcacgcgccg accgggacgc ccatcgtcgt
     7501 ggcgggcaag cgcacgcgca ccgtggggcg cgtgtcgatg gacatgctgt tcgtcgacct
     7561 gggcggcatc gccaacgcat cggtgggctc gaacgtgatc ctctggggcg agcacgtctc
     7621 cgcggacgag gtggccgcgg cggccggcac cgtgagctac gagctgctgt gcgggctcgg
     7681 gggccgcgtc ccggtcagcg aaatctaggc gcgattgctg taatctgccg cccgtggcga
     7741 aggcgaagac cgtttacgtc tgcaccgagt gcggcggcca gtctccgaag tggcaggggc
     7801 agtgcccggc gtgcgccgcg tggaacacgc tggtcgaggc ggcggccgag gcgccggcgc
     7861 ggcaccgctt cgccggcgtc gcgcgcgcct cgggcctgca gcgcctcgcc gacgtcgcgg
     7921 ccaaggagtc gcaccgcacg ccgaccggga tcgaggagct cgaccgcgcg ctcggcggcg
     7981 gcctggtcgc cgggcaggtg gtgctgatcg gcggcgatcc cggcatcggg aaatccacgc
     8041 tcctgctgca ggcgctcgcg cggctgtcgg ccgagacgcg cgcgctctac gtctcgggcg
     8101 aggagtccgc ggagcaggtc gcgctgcgcg cccagcgcct gcagctcgac tgcgggaagc
     8161 tcgaggtgct ggccgagatc cagctcgagc gcatcctcga ggcgctcgag agcgcgaagc
     8221 cgaagatcgc agtggtcgac tccatccaga ccctgtattc cgaggcgctc acctcggcgc
     8281 cgggctcggt cgcgcaggtg cgcgagtgcg tggcgcagct cgcgcgctgg gccaagcagt
     8341 cgggcacggt catcatccag gtcggccacg tgaccaagga gggggcgctc gcggggccgc
     8401 gcgtggtcga gcacatcgtc gacaccgtgc tctacttcga aggcgacacg cagcagagct
     8461 tccgcgtcgt gcgcgcgttc aagaaccgct tcggcgcggt gaacgagctg ggcgtgttcg
     8521 cgatgaccga gcgcggcctg aagggcgtgt cgaacccgtc ggcgctgtgt ctctcgggac
     8581 acagcggcga ggccgcgggc tcgtgcatca tggtgacgca ggagggcacg cggccgctgc
     8641 tggtcgagat ccaggcgctg gtcgacgccg cgcaggcgcc caacccgcgc cgcctcggcg
     8701 tcggcatcga gcagaaccgc ctggcgatgc tgctcgcggt gctgcaccgg cacgcgggca
     8761 tctcgtgcgg cgaccaggac gttttcgtga acgcggtcgg cggcgtgcgc atcgccgagc
     8821 cggccgcgga cctcgcgatc ctgctcgcgg tcgtgtcgtc gctcgccgac aagccgctcg
     8881 cgccgaagct cgtcgtcttc ggcgaggtcg ggctcgcggg cgaggtgcgt cccgcgccgc
     8941 gcgggcagga gcgcctgaag gaagcggcca agctcggctt cgcgcaggcg atcgtcccgc
     9001 gcgcgaacca gccgcgccag ccgatcgagg gtctcgaggt gatcgcggtc gaccgcctcg
     9061 ccgacgccat cgtgcgcttc cgcagctagg cggcgccggc acgtttcatg ccgtcgtcat
     9121 agaccgacgg ccgcggcggc ggcgcggggc tgcgaaccga accgggcccg cgcgtgtcct
     9181 atgcgctgat tgcggctaaa caaccgcttg cgcggaaaag agcggcggaa cctgtcaaag
     9241 acaacagggt ttcgcacgcc gcgcgactgg cgcacaatga gaatggagat acgaagcgca
     9301 tcccatcctg ccgcccgctc catgtcccac gacatgatca cccccgaatc ggctccgcgc
     9361 acggacgacg agcgcctcac ggccgcgcgt gccgccgcgc acggcccgct gccgcaggag
     9421 ctggaggacc ccttccgcga cctggtcgcg gcggtcgagg aatacgcgat cttcctgctc
     9481 aacgcgaaag gcgagatcca gagctggaac gcgggcgccg agcgcatcaa aggctacgag
     9541 gcgcacgaga tcctcggccg gcacttctcc gtcttctatc cgcccgagtc ggtggcctcg
     9601 ggctggcccg atcacgagct gcgcgtggcc gaggccgccg gccgcttcaa cgacgagggc
     9661 tggcgcctgc gcaaggacgg caaccgcttc tgggcgagcg tcacgatcac ggcgctgcgc
     9721 caccgcgacg gcgcgctgcg cggcttcctc aagatcacgc gcgacctgac cgagcgcaag
     9781 cgcgccgagg agggcctgcg ccagagcgag gagcgcttcc gcctgctgat cgagggcgtg
     9841 caggactacg ccatctacat gctcgacccg aacggcaacg tcgcgagctg gaacgtcggc
     9901 gccgagcgca tcgagggcta ccgggccgag gagatcatcg gccggcactt ctcggccttc
     9961 tatccgcccg aggcggtcgc tcaggggcgg cccgactggc agctgcgcac ggcgctggag
    10021 caggggcgcg ccgaggacga gggctggcgc acgcgcaagg acggcacgca gtactgggcg
    10081 aacgcggtga tcaccgccct caaggacaag gacggcacgc tgcgcggctt cgccaagatc
    10141 gtgcgcgaca tgaccgagcg ccgcaagatc gaggctctgc agcgcgccga tcgccagaag
    10201 aacgagttcc tggcgctgct cgcgcacgag ctgcgcaacc cgctcgcgcc gatccgcaac
    10261 gcgctctaca tcatggggca gcccgaggcg acctcggcga acgcgacgcg cgcgcgagag
    10321 atcgccgagc gccaggtgca gcacatgtcg cggctgctcg acgacctgat cgacgtcgcg
    10381 cgcgtgagcc gcggcaccat ggagctccgg aaagagctga tcgacgtcga cgtcgcggtc
    10441 gcctccgcgg tcgaggcggt gcggccgctg gtccaggacc agcgccacca gctcaccgtc
    10501 gccgtgccgc cgcgcacgct gtgggtcgat gccgacccgg cgcgcctcga gcaggtcctg
    10561 atcaacctgc tgaccaacgc gatcaagtac accgacccgg gcgggcatat ctggatcacc
    10621 gccgagacgc agggcgagga ggtggtgctg cgcgtgcgcg actcgggcat cggcgtcgac
    10681 ccggtgatgc tgccgcgcat cttcgagctg ttcgtgcagg ccgagcgccg catggaccgc
    10741 gccgtcggcg gcgtcggcat cgggctctcg ctcgtcaaga agctgatcga gctgcacggc
    10801 ggcaccgtgc aggcgttcag cgccggcctc ggcaaaggca ccgagctggt ggtgcgtctg
    10861 cccgcggcga aaacgcccga ggcgggcggc gcccagtcgc gcacggacgg cgtgccttcc
    10921 gacctgttcg ggctgcgcat cctggtggtg gacgacaaca tcaacgccgc cgacagcctc
    10981 gcgacgctgc tcgagctcgc cgggcacgaa gtgcgcgtcg cctacgaggg cgaggccgcg
    11041 ctgctggtgg ccgaggcgtt caagccgcag gtcgtgctgc tcgacatcgg catgccgggg
    11101 atggacggct acgaggtcgg gcgcgcgctg cgccagaagc cgcagacgcg ctcggcgttg
    11161 ctggtcgcga tcacgggctg gggcgcgccc gacgacctgc gccgctcgaa ggaagccggc
    11221 ttcgaccatc acctggtgaa gccggtcgag ccggccgcgc tgcagcgcct gctcgcgcgc
    11281 cgctccatac gcacccgcta gggcgcgtcg ttccgagctg cgctaaggcg tgatcgcgcg
    11341 cgccgcggcg agcccgcttc gcaccgccgt ttccagcgtt cccggatagt cgctcgcgac
    11401 gtaatcgccc gcgagcagca gcccgggcac gggcgtgacg gtcgccgggc gctcgagcgc
    11461 cggcgcgcac gagaaggtcg cgcgccgctc ggcgatcgtg cgcgaccaca gcggcgccgg
    11521 cagcctcccc cacgccgcct cgatctcgcg ctccaccgcg gcggtcagcg ccggcgactc
    11581 catctccgcg tgccgtcccg agccgctgat caccgcggcg agcagcccgg gtggcccgcc
    11641 gagccggccg cggtcgaaga cccactggat cagcccgccg gcgaagccga tcatcggcgc
    11701 cgagagcgcg accgagtcgg ggtactgcag gtagcacgtg acgatgggct cgtactcgaa
    11761 gcgcccgagc gccgcgcgca cgccttcgag gccgctccat ttcggcagca gcgccgcgac
    11821 gtgctggggc gcgcacgcga cgacgacgtg cgagtacgaa tccgcctcgt cgtcgaggcg
    11881 gaagcggccc ggctcgcc
//
LOCUS       DATDMW010000102         9582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2145108, whole genome shotgun sequence.
ACCESSION   DATDMW010000102 DATDMW010000000
VERSION     DATDMW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9582
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145108"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..367
                     /gene="gloA"
                     /locus_tag="VI319_04490"
     CDS             2..367
                     /gene="gloA"
                     /locus_tag="VI319_04490"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY6863137.1"
                     /translation="MLRVGDLERSLRFYTEVLGMKLLRRNDYPEGKFTLAFVGYGAES
                     ETAVLELTHNWGVEKYELGTAYGHVAIAVDDARKACDEVTKRGGKVTREAGPMKHGTT
                     VIAFVEDPDGYKIELIERK"
     gene            complement(369..1034)
                     /locus_tag="VI319_04495"
     CDS             complement(369..1034)
                     /locus_tag="VI319_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863138.1"
                     /translation="MGLLDDLKKQAEMLQTQQNVQQSVHADRIKLVEEKMKQTFQYVH
                     ELLKQLSVLKPTSPLVFYIMGVGDLKNLSFAEQFSDFRRKRINDKEYFDTINFYIKWA
                     SNNTMVVDRDNPPMAIKTREALYASKVKFTEEELKNPKGFVTGAKFIIPSVVITDVQI
                     KADHDQGRLLMMTKNLLGLGPDTFALPAPEINEALLEEFGKALIGQPNNFRRFRGVAP
                     APR"
     gene            complement(1089..1868)
                     /gene="rsmA"
                     /locus_tag="VI319_04500"
     CDS             complement(1089..1868)
                     /gene="rsmA"
                     /locus_tag="VI319_04500"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY6863139.1"
                     /translation="MRHVARKRFGQHFLEAPEIVRAIVDAMALRPDDSVVEIGPGLGA
                     LTATLLDRVRHLHVVEIDRDLCARLRQRFTPERLTVHEGDALAFDFASLGAPLRVVGN
                     LPYNISTPLLFHLMGAAGRCVDLHLMLQKEVVDRMAADPGSKDYGRLTVMLQYRYRLE
                     RLLDVPASAFRPRPEVDSAVVRLVPRPVRELSALDADAFSRVVKTAFSTRRKIVKNSL
                     SSLVSAPQLEALGIDPTSRAEDLPVSDFIAIANGMHEAGPR"
     gene            complement(1880..2851)
                     /gene="pdxA"
                     /locus_tag="VI319_04505"
     CDS             complement(1880..2851)
                     /gene="pdxA"
                     /locus_tag="VI319_04505"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY6863140.1"
                     /translation="MIAVTSGEPAGIGPDICLALARDELAARVVVLGDRHLLEQRAAA
                     LGIEVRLSEAGSTWRPGALAVEHVPLAVPSIPGVLDPRNARYVIAQLDRALDGCLSGR
                     FAAMVTAPVHKAAINDAGIAFTGHTEFLAERTRTPHVVMMLAGGGLRVALATTHMAVK
                     DVPGALSPESLEATIRVLAADLRRRFGVESPRILVAGLNPHGGEGGHFGREEIELIAP
                     VLERLRAEGLRLAGPLPADTLFTPDVLSQGDCVLAMYHDQGLPVLKYASFGRGINITL
                     GLPIIRTSVDHGTALDLAGTGKADAGSLLEAVRAAAELWRTAAGVPR"
     gene            complement(2901..4283)
                     /locus_tag="VI319_04510"
     CDS             complement(2901..4283)
                     /locus_tag="VI319_04510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6863141.1"
                     /translation="MSRIFLFAALLWPLAALAQDGAPAPAAAPAATPAVPTPSVAPAA
                     PRAPASGKMELVDRIVAVVNNEVITQFELSERVGRILKELAQRGTPLPPRDQLERQVL
                     ERMILDKVQLQLARDTGLRVDDLDLDKTIARIAEGNNISLTEFRTRLERDGIAFDKFR
                     EDVRTEIVLSRLREREVDNKITVAESEIDNFLADQGATAKGAPVEYNVAHILLRVPEQ
                     ARPDQLEQQRSRALEVVKQLRGGADFAQVAAVYSDAPDALRGGAMGWRSQDRLPELFV
                     EALAKLQPGDVSDVLRSPAGFHVLKLLDRRGASALSEVEQFHVRHILVRTNELVSEAD
                     ARRKLTQLRERIANGADFGELARLHSDDASASRGGDLGWIYPGDTVPEFERAFMHLKD
                     GEVSEPVKTPFGWHLIQVLEHRTADASAERKRLDARKILRERKSDEAYQEWLRQLRDR
                     AYVEYRLEDR"
     gene            complement(4300..6573)
                     /locus_tag="VI319_04515"
     CDS             complement(4300..6573)
                     /locus_tag="VI319_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEY6863142.1"
                     /translation="MSFSGARWLAAATIALAPAGVCAQDLGLKLKLQRTWVGPPSSTA
                     LVPTFIFADRLEGTQGEEARAEGSAELRKGDTNVEADSLRYFQQREDVEAVGNVRIER
                     TGDVITGPSLKYNMNSGTGAFDHPDFTLAPRSKTGVAGVEARGNASSIDFLGEDRFKV
                     HDVFFTTCKPGNEDWYVQARELDLDYTRQVGTARGGKVVFKGVPIIPAPYLDFSLNNQ
                     RKSGFLPPHVGTTGKSGAEFSTPYYFNLAPNYDATLTPREMAKRGLQLAAEARYLEPT
                     YFGEYRGEVLPNDQARGGETRSAQTFVHTYRNGPVTGGLNLNKVSDDFYFIDLASRIN
                     ITSQVNLIREGFLTYGGAWAGTGGYSLTGRVQSYQTLQTDPDNPVPIPYARRPQLALS
                     AVRQDLPGGVDFGMSGEFVDFSHPSNVVGTRAIAYPSFTLPVIRPSSYITPKIGLHIT
                     QYELSRQDPSVPSAINRTLPIASLDSGLLFERDTQWRGQSFTQTLEPRAYYLYVPFRD
                     QNNIPLFDTAVADFNYAQIFSENAFAGGDRINDANQLTLGVTSRMLVAATGQEALRAT
                     IAQRYYFEDQRVTLTPTDTPRTYHSSDYLASISGRASRDWTVEASEQYNPRDSRTERL
                     TLSGRYQPSPLHTVNLSYRYLRDQIAQVDISSQWPLGGGWYGVGRFNYSTSDKRIVEA
                     LAGIEYNAGCWISRVVIQHFALTAGSSTSTLFLQLELNGFSRIGSNPLEALKRNVPGY
                     QTLGAPAAPDQPARPFD"
     gene            6672..7649
                     /locus_tag="VI319_04520"
     CDS             6672..7649
                     /locus_tag="VI319_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY6863143.1"
                     /translation="MRQWLEGVLGTPGFDLAPASGDASFRRYFRVTRSSSDTLIAMDA
                     PPEKEDCRPFLHAAALFADAGVHVPAVHAQNLAEGFLLLSDLGSTTYLQALDSDSADR
                     LYGLAIAALVRIQRASAAGRLPEYDRELLLRELKLFPEWYVARQLGRELTPAQRATLD
                     AAFELLLENNLAQPRVYVHRDYHSRNLMVCEPGPGILDFQDAVYGPITYDLVSLLRDA
                     YIEWDEERVLDWAIVYWEAARKAGLPVAGDFAAFYRDFEWMGVQRQLKVIGIFARLCH
                     RDGKEGYLKDQPLVMRYLRRACERYRELAPLAQLLGELQDERRQARHSF"
     gene            7667..8332
                     /locus_tag="VI319_04525"
     CDS             7667..8332
                     /locus_tag="VI319_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY6863144.1"
                     /translation="MKAMLLAAGRGERMRPLTDTTPKPLLEVRGKPVIVWTLERLARA
                     GFRELVINHAHLGHKIEQSLGGGSRFGVSIRYSPEGEALETAGGIANALPLLGPEPFV
                     VVNGDIYCDYDFSRLRGRSLGKNLAHLVLVPNPEHNPGGDFVLDASEIRMEGGPKHTF
                     SGIALYRPELFHGLERGSKGRMLPLWVEAIGQRRVGGELHAGVWHDVGTPQRLAALNQ
                     PGA"
     gene            8424..>9582
                     /locus_tag="VI319_04530"
     CDS             8424..>9582
                     /locus_tag="VI319_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY6863145.1"
                     /translation="MGAGIAVVPTAPERIRNRDSDYLYRFDSYFYYLTGFPEPDAVLV
                     LLAGEPPRTILFCRERNPERELWDGLRYGPEGAKETFGVDEAYSITEIDERLPKLIAD
                     QPALHYPVGADAGWDARMMGWLNQVRALARTGVAAPAAMHDVRVALDEMRLFKDDAEL
                     AVMRRAGAISAGAHERAMRAARPGRWEYEIEAELLYEFRRRGAQCPAYWPIVAGGANA
                     CILHYRENNAQLAQDDLLLIDAGCELDGYAADITRTFPVGGRFTPAQRDIYELVLAAQ
                     AAAIAAVAPGARWNAPHDAAVRVLAQGFVDLGLCAGDVEHVIQSEDYKRFYMHRTGHW
                     LGLDVHDAGDYKRGGEWRALETGMVLTVEPGCYIRPAENVPQRYWNIGVRIE"
BASE COUNT         1501 a         3220 c         3311 g         1550 t
ORIGIN      
        1 catgcttcgc gtcggcgacc tcgagcgctc gctgcggttc tacaccgagg tcctcgggat
       61 gaagctgctc cggcgcaacg attacccgga aggcaagttc acgctcgcct tcgtcggcta
      121 cggcgcggaa tccgagaccg cggtgctcga gctgacccac aactggggcg tcgagaagta
      181 cgagcttggc accgcctacg gccacgtcgc gatcgcggtc gacgacgcac gcaaggcgtg
      241 cgatgaagtg acgaagcgcg ggggaaaggt cactcgcgag gcgggtccca tgaagcacgg
      301 gaccacggtg atcgccttcg tcgaggaccc cgacggctac aagatcgagc tgatcgagcg
      361 gaagtagctc agcgcggcgc cggcgccacg cctcggaagc ggcggaagtt gttcggctgg
      421 ccgatcagcg ccttgccgaa ctcctcgagc agcgcttcgt tgatctccgg ggcgggcagc
      481 gcgaacgtgt cgggccccag accgagcagg ttcttggtca tcatcagcag cctgccctgg
      541 tcatggtcgg ccttgatctg gacgtcggtg atgacgaccg acgggatgat gaacttcgct
      601 ccggtgacga agcccttggg gttcttcagc tcttcctcgg tgaacttcac cttgctcgcg
      661 tagagcgcct cgcgcgtctt gatcgccatg ggcgggttgt cgcggtcgac caccatggtg
      721 ttgttcgagg cccacttgat gtagaagttg atggtgtcga aatattcctt gtcgttgatg
      781 cgcttgcgcc ggaagtcgct gaattgctcg gcgaacgaca ggttcttcag gtcgcccacg
      841 cccatgatgt agaagacgag cggcgaggtg ggcttcagca ccgacagctg cttgagcagc
      901 tcgtggacgt actggaacgt ctgcttcatc ttctcttcga ccagcttgat gcggtcggcg
      961 tgtaccgact gctgcacgtt ctgctgcgtc tgcagcatct cggcctgttt tttcagatcg
     1021 tcgagaaggc ccatggcgta accccgtccc tgattgtcac ggctacccgg gtattgtgcc
     1081 agcagcgctt accggggtcc agcttcgtgc atgccgttgg caatcgcaat gaagtcggag
     1141 acaggaagat cttccgcgcg cgatgtcgga tcgatgccca gcgcctcgag ctgcggggcc
     1201 gataccaggc tggagagtga atttttcacg atcttgcgac gggtcgagaa cgccgttttc
     1261 accacgcgcg agaacgcgtc cgcgtccaga gccgagagct cccggacggg acgcggcacc
     1321 aggcgcacca ccgccgagtc gacttcgggt cgggggcgga aagccgaagc cgggacatcg
     1381 agcaggcgct cgagacggta ccggtactgc agcatgaccg taaggcgtcc gtaatccttg
     1441 gatccgggat cggcagccat ccggtcgacg acctccttct gcagcatcag atgcagatcg
     1501 acacagcgcc cggccgcccc catcaggtgg aacagcaggg gcgtcgatat gttgtacggc
     1561 agattgccca cgacccgcag cggcgcgccc aggctcgcga agtcgaacgc cagcgcgtcg
     1621 ccctcgtgga ccgtcaggcg ctcgggcgtg aagcgctggc gcagccgagc gcagaggtcg
     1681 cgatcgatct cgacgacgtg caggtgccgc acgcgatcga gcagcgtcgc ggtgagagcg
     1741 cccagcccgg ggccgatctc caccaccgaa tcgtccgggc gcaacgccat cgcgtcgacg
     1801 atcgcgcgga cgatctccgg ggcctcgaga aagtgctggc cgaaccgctt tcgcgcgacg
     1861 tggcgcatcg gcggtcgcgc tagcgcggga cccccgccgc cgtgcgccac agctcggctg
     1921 cggcgcgcac cgcctcgagc aggctgccgg cgtcggcctt tccggtcccg gcgaggtcca
     1981 gggccgtgcc gtgatccacc gacgtccgga tgatcggcag gccgagcgtg atattgatgc
     2041 cgcggccgaa gctcgcgtac ttcagcaccg gcagcccctg gtcgtggtac atcgcgagca
     2101 cgcaatcgcc ctgcgacagc acgtcgggcg tgaacagcgt gtccgcaggg agcggcccgg
     2161 ccaggcgcag gccttcggcg cgcaacctct ccagcaccgg agcaatgagc tcgatctcct
     2221 cgcggccgaa gtgtccgcct tcgccaccgt gcgggttgag cccggcgacg aggatccgcg
     2281 gcgattcgac tccgaagcgc cggcgaagat cggcggcgag cacgcgtatc gtcgcttcga
     2341 gactctcggg cgagagcgcc cccggcacgt ccttcaccgc catgtgcgtc gtcgcgagcg
     2401 cgacccgcag gccgcccccc gcgagcatca tcacgacgtg cggcgtgcgc gtgcgctcgg
     2461 cgagaaactc ggtgtgaccg gtgaaggcga tgccggcgtc gttgatcgcc gccttgtgca
     2521 ccggcgccgt gaccatggcg gcgaacctcc cgctcaggca gccgtcgagc gcccgatcga
     2581 gctgcgcgat cacatatcgc gcgttgcgcg gatcgagaac gccggggatg gacgggaccg
     2641 cgaggggaac gtgctcgacc gcgagtgcgc cgggacgcca cgtggatccg gcctccgaga
     2701 gccggacttc gattccgagc gctgccgcgc gctgctccag cagatgccgg tcgccgagaa
     2761 cgacgacgcg cgcggcgagc tcatcgcgcg cgagggcgag gcagatgtcg ggaccgatgc
     2821 cggccggctc tcccgacgtc accgcgatca cgggtccggg ttccctcgca gacaaagtcg
     2881 cgccgcggcg ggcgcctgtc tcagcggtct tcaagccggt actcgacgta cgcgcggtcg
     2941 cgcagctgcc tgagccattc ctggtacgcc tcgtcgctct tgcgctcgcg caggatcttg
     3001 cgcgcgtcga ggcgcttgcg ctcggcggac gcgtccgcgg tgcgatgctc gagcacctgg
     3061 atcaggtgcc agccgaaagg cgtcttcacg ggctcgctca cctcgccgtc cttcagatgc
     3121 atgaaggcgc gctcgaactc gggcaccgta tcgcccgggt agatccagcc gaggtcgccc
     3181 ccgcgcgagg ccgaagcgtc gtccgaatgc agccgggcga gctcgccgaa gtccgcgccg
     3241 ttcgcgatgc gctcgcggag ctgcgtgagc ttgcgcctgg cgtcggcctc ggacacgagc
     3301 tcgttggtgc gcacgaggat atgccgaacg tggaactgct cgacctcgct cagcgccgag
     3361 gcgccgcgcc gatcgaggag cttgagcacg tggaaccccg cggggctgcg cagcacgtcg
     3421 gacacgtcgc ccggctgcag cttcgcgagg gcctcgacga agagctccgg cagccggtcc
     3481 tgcgagcgcc agcccatcgc accgccgcgc agcgcgtcgg gcgcgtccga gtacacggcg
     3541 gcgacctgtg cgaaatccgc tccgccgcgc agctgcttca ccacctcgag cgcgcggctg
     3601 cgctgctgct cgagctggtc gggccgcgcc tgctcgggaa cgcgcagcaa tatgtgcgcg
     3661 acgttgtact ccaccggcgc gcctttcgcc gtggcgccct ggtcggcgag gaagttgtcg
     3721 atctcgctct ccgcgacggt gatcttgttg tcgacctcgc gctcgcgcag cctcgacagg
     3781 acgatctcgg tgcgcacgtc ctcgcggaat ttgtcgaagg cgattccgtc tctctcgagc
     3841 cgggtcctga actcggtaag cgaaatgttg ttgccttcgg cgatgcgcgc gatcgtcttg
     3901 tcgaggtcga ggtcgtcgac gcgcaagccc gtgtcccggg cgagctgcag ctggaccttg
     3961 tcgaggatca tccgctcgag cacttgccgc tcgagctggt cgcgcggcgg cagcggcgtt
     4021 ccccgctgcg ccagctcctt caggatgcgg cccacccgct cggacaactc gaactgcgtg
     4081 atgacctcgt tgttcaccac ggcgacgatg cggtcgacga gctccatctt cccggacgcg
     4141 ggcgcgcgag gggcggcagg cgccacggag ggcgtcggca ccgcgggcgt tgcagcgggc
     4201 gccgcggccg gcgccggtgc accgtcctgg gccagcgcgg cgaggggcca cagcagcgcc
     4261 gcgaagagaa atatcctgga catccgaatc gatccgatat cagtcaaaag gccgcgccgg
     4321 ctggtcgggc gccgccggag cgccgagggt ctggtagccc gggacgtttc gcttcagcgc
     4381 ttcgagcgga ttcgacccga tgcgggagaa gccgtttagc tccagttgga gaaacagcgt
     4441 gctcgtcgac gagcccgcgg tcagggcgaa gtgctggatc acgacgcgcg atatccagca
     4501 ccccgcattg tattcgatcc cggccagggc ttcgacgatt cgcttgtcgc tcgtcgagta
     4561 gttgaagcgg ccgacgccgt accagccgcc cccgagcggc cattgcgacg agatgtcgac
     4621 ctgcgcgatc tggtcgcgca gatagcggta gctcaaattc accgtatgca gcggactggg
     4681 ctgatagcga cccgacaggg tcaggcgctc ggtccgcgag tcgcgcgggt tgtactgctc
     4741 ggacgcttcg accgtccagt cgcgcgacgc gcgtccggag atcgaggcga gataatcgga
     4801 gctgtgatag gtacgcggcg tgtcggtcgg ggtgagcgtc acgcgctggt cctcgaagta
     4861 gtagcgctgg gcgatggtcg cgcgcagcgc ctcctgaccg gtggcggcga ccagcatccg
     4921 cgaagtgact ccgagcgtga gctggttggc gtcgttgatg cggtcgccgc ccgcgaaggc
     4981 gttctccgag aaaatctgcg cgtagttgaa gtcggcgacg gcggtgtcga agagcgggat
     5041 gttgttctgg tcccggaacg gcacgtacag gtagtaggcg cgcggctcga gcgtctgggt
     5101 gaacgattgg ccgcgccact gcgtgtcgcg ctcgaacagc aggccgctgt cgaggctggc
     5161 gatcggcagc gtgcggttga tcgcgctcgg caccgagggg tcttgccggc tcagctcgta
     5221 ctgcgtgatg tggagcccga tcttcggcgt gatgtaggac gaaggccgga tcaccggcag
     5281 cgtgaagctc gggtacgcga tcgcccgggt tccgaccacg ttgctgggat ggctgaagtc
     5341 cacgaactcg cccgacatcc cgaagtcgac gccgccgggc aaatcctggc gcaccgcgga
     5401 cagcgccagc tgcggccgcc gggcgtaggg aatcggcacc ggattgtcgg gatcggtctg
     5461 cagggtctgg tagctctgga cgcgccccgt gaggctgtag ccgccggtcc ccgcccaggc
     5521 gccgccatag gtcaggaatc cctcgcggat gaggttcacc tgcgacgtga tgttgatgcg
     5581 gctcgcgaga tcgatgaagt agaagtcgtc cgacaccttg ttcaggttca gaccgccggt
     5641 cacgggtccg ttgcggtaag tgtggacgaa ggtctgcgcc gagcgcgtct cgcccccgcg
     5701 cgcctggtcg ttcggcagca cctcgccccg gtactcgccg aaataggtcg gctcgaggta
     5761 gcgggcctcg gcggcgagct gcagaccgcg cttggccatt tcgcgcggcg tcagcgtcgc
     5821 gtcgtagttc ggcgcgagat tgaagtaata cggcgtcgag aattccgcgc cgctcttgcc
     5881 ggtggtcccc acgtgcggag gcaggaagcc cgacttgcgc tggttgttga gcgagaaatc
     5941 cagatagggc gccgggatga tcggcacgcc cttgaacacg accttgcctc cgcgcgccgt
     6001 gccgacctgg cgcgtgtagt cgaggtcgag ctcgcgcgcc tgcacgtacc agtcctcgtt
     6061 tcccggcttg cacgtcgtga agaagacatc gtggaccttg aagcggtcct cgcccagaaa
     6121 gtcgatcgac gaggcgtttc cgcgcgcctc gacccccgcc accccggtct tgcttcgcgg
     6181 cgcgagggtg aagtcgggat gatcgaaggc cccggtaccg ctgttcatgt tgtacttcag
     6241 cgagggaccg gtgatcacgt cacccgtgcg ctcgatgcga acgttgccga ccgcctcgac
     6301 gtcctcgcgt tgctggaaat agcggagcga gtccgcctcg acgttggtgt cgcccttgcg
     6361 cagctcggcg ctgccctcgg cgcgggcctc ctcgccctgc gtgccttcga gccgatcggc
     6421 gaagatgaaa gtcggcacca gcgccgtcga cgacggcggg ccgacccacg tgcgctgaag
     6481 cttgagcttc agcccgaggt cttgtgcgca cacgcccgcg ggcgccagcg cgatcgtcgc
     6541 cgccgccagc cagcgggctc cggaaaaact catgaaattc ttggaattat cctcgtgtgc
     6601 gcgaatgcta aaatcgcaca aatcgagagc gaaggcaatt tcgggacggc ggattggatc
     6661 ggctagcgca gctgcgtcaa tggctggaag gcgtgctcgg cacccccggc ttcgatctcg
     6721 cgcccgcgtc gggagacgcg agcttccggc gctacttccg ggtgacccgg tcctcgagcg
     6781 acaccttgat cgcgatggac gcgccgcctg agaaagagga ctgccggccc tttctccacg
     6841 ccgcggccct gttcgctgac gccggcgtcc acgtgccggc cgtgcacgcg cagaaccttg
     6901 ccgaaggctt tttgctgctc tccgacctcg gcagcaccac gtatctgcag gcgctggatt
     6961 cggacagcgc ggatcggctg tacggacttg cgatcgcggc gctcgtccgg atccagcgcg
     7021 cgagcgccgc gggaaggctt cccgaatacg accgcgagct gctcctgcgc gagctcaagc
     7081 tctttcccga gtggtacgtg gcgcgccagc tgggacgcga gctcaccccg gcgcagcggg
     7141 cgacgctcga cgccgcgttc gagctgctgc tcgagaacaa tctcgcgcag ccccgggtgt
     7201 acgtgcaccg ggattaccac tcgcgcaacc tgatggtgtg cgagccgggt cccgggatac
     7261 tcgacttcca ggacgcggtg tacgggccga tcacctacga cctcgtctct ctgcttcggg
     7321 acgcttacat cgaatgggac gaggagcgcg ttctcgactg ggccatcgtg tactgggaag
     7381 ccgcgcgcaa ggcggggctt cccgtcgcgg gcgatttcgc ggcgttctac cgcgacttcg
     7441 aatggatggg tgtgcagcgg cagctcaaag tcatcggcat cttcgcccgg ctgtgccacc
     7501 gcgacggcaa agagggttat ctcaaggacc agccgctggt catgcgctac ctgcgccgcg
     7561 cctgcgagcg ctatcgcgag ctcgcgcctc tcgcgcagct gctcggcgag ctccaggacg
     7621 agcggcgcca agcgcgccat agcttctgag catcgatggt gcacccatga aggcgatgct
     7681 actggcggcc gggcgcggcg agcgcatgcg gccgctgacc gacacgacgc cgaagccgct
     7741 gctcgaggtg cgcggcaagc ccgtcatcgt ctggaccctc gagcgcctgg cgcgcgcggg
     7801 gttccgcgag ctcgtcatca atcacgccca tctcgggcac aagatcgagc agtcgctggg
     7861 tggcggaagc cgcttcggag tctcgatacg ctattcgccc gagggcgagg cactcgagac
     7921 cgcgggcggg atcgccaacg cgctgccgct cctcggaccc gagcccttcg tggtcgtgaa
     7981 cggcgacatt tactgcgact acgacttctc gcgcctgcgc ggacgatcgc tcgggaagaa
     8041 cctcgcgcac ctcgtgctcg tgccgaaccc cgagcacaat cccggcggcg atttcgtcct
     8101 cgacgcctcg gagatccgga tggagggagg ccccaagcac accttcagcg ggatcgcgct
     8161 gtaccggccc gagctgttcc acgggctgga gcggggctcc aagggcagga tgctgccgct
     8221 gtgggtcgag gcgatcggac agaggcgcgt cgggggcgag ctgcacgcgg gcgtctggca
     8281 cgacgtcggg acgccgcagc gcctggcggc gctgaaccag ccgggcgctt gatcccggcc
     8341 gcccggcgga cgacaatccg gcgatggacg ccgcaacgca tctctcccgg tacgcggagc
     8401 gccgccggcg gttgagccgg cagatgggcg ccgggatcgc agtcgtgccg accgcaccgg
     8461 agcgcatccg caatcgcgac agcgactacc tgtaccgctt cgacagctat ttctactatc
     8521 tcaccgggtt tcccgagccc gacgccgtgc tggtgctcct cgccggcgag ccgccgcgca
     8581 cgatcctgtt ctgccgcgag cgcaaccccg agcgcgagct gtgggacggg ctgcgctacg
     8641 gcccggaagg cgccaaagaa acgttcggcg tcgacgaggc ctatagcatc accgagatcg
     8701 acgagcgcct gcccaagctg atcgccgacc agcccgcgct ccactatccg gtcggagccg
     8761 acgccggctg ggacgcgcgc atgatgggct ggctgaacca ggtgcgcgcc ctcgcgcgca
     8821 cgggcgtcgc ggcgccggcg gcgatgcacg acgtgcgcgt cgcgctcgac gagatgcggc
     8881 tgttcaagga cgacgccgag ctcgcggtca tgcgccgcgc cggcgcgatc tccgcgggcg
     8941 cgcacgagcg cgcgatgcgc gccgcgcgcc cgggccgctg ggagtacgag atcgaggccg
     9001 agctcctgta cgagttccgc cggcgcgggg cgcagtgccc ggcgtactgg cccatcgtcg
     9061 cgggcggcgc caacgcctgc atcctccatt accgcgagaa caacgcgcag ctcgcgcagg
     9121 acgacctgct gctcatcgac gcgggctgcg agctcgacgg ctacgcggcc gacattacgc
     9181 gcacgttccc ggtcggcgga cgcttcacgc ccgcgcagcg cgacatctac gagctcgtgc
     9241 tcgccgcgca ggccgccgcc atcgccgccg tcgcgcccgg ggcccgctgg aacgcgccgc
     9301 acgacgcggc ggtgcgcgtg ctggcgcagg gcttcgtcga cctgggccta tgcgcgggcg
     9361 acgtcgagca cgtgatccag agcgaggact acaagcgctt ctacatgcat cgcactggcc
     9421 actggctcgg tctcgacgtc cacgacgccg gcgactacaa gcgcggcggc gagtggcgcg
     9481 cgctcgagac cggcatggtg ctgaccgtcg agcccggttg ctacatccgg cccgcggaaa
     9541 acgtgccgca gcgctactgg aacatcggcg tgcgcatcga gg
//
LOCUS       DATDMW010000103         7491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1896809, whole genome shotgun sequence.
ACCESSION   DATDMW010000103 DATDMW010000000
VERSION     DATDMW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896809"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1055)
                     /locus_tag="VI319_04535"
     CDS             complement(138..1055)
                     /locus_tag="VI319_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY6863146.1"
                     /translation="MQHIYIHEVVARDGFQIEPQFVPTERKIELLDALSETGVAKIEV
                     TSFVSPKAVPNLRDAEEVMAGIRRNPAVRYVALIANAKGVERAAAARVDEVNLVVSVS
                     ETHNRANVRRSTDASFEGFAEILRALDGTGIDVTGSLSTAFGCPFEGDQAAGRVMEFV
                     RRYVDLGVDAVTLADTTGMAHPRQVESLASAVREAFPELPVALHFHNTRGMGLANVLA
                     GLAAGVRRYEGCLGGLGGCPFAPGATGNICTEDLVHMLEAMGYGTGVDLDRLLAASRR
                     LGDIVRHDLPGQVVKAGKATELHPAPQGS"
     gene            complement(1075..2274)
                     /locus_tag="VI319_04540"
     CDS             complement(1075..2274)
                     /locus_tag="VI319_04540"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY6863147.1"
                     /translation="MTPGALAGLKVIELGQLIAGPFAGKFFAELGAEVIKIEPPEGDP
                     LRKWRKLHEGTSLWWYVQNRNKKSVTADLRLPEGQEVVRRLARGADVVIENFRPGTME
                     KWGLGYERLADGNPGLVMLRLSGFGQTGPYRDQAGFGAIGESMGGLRYVTGFPDRPPV
                     RPNLSIGDALAALHGVIGTLAALRHRDAGGLRSGKGQVVDVALYEAVFNMMESTLPEY
                     DLFGEVRERTGSNLTGIVPSNTYLTREGRHVVIGANADSIFKRLMNLIGRPDLAEDPA
                     LADNAGRAKRADELDAVIGEWTSRHGAEDVLRMLNEAKVPSGKIYSIADIARDPQYLA
                     RDMIREIALPDGARVKVPGVVPKLSATPGDIGRPGPRLGEHTEAVLAAHGYGAAEIAQ
                     LRGRRAV"
     gene            complement(2271..2873)
                     /gene="recR"
                     /locus_tag="VI319_04545"
     CDS             complement(2271..2873)
                     /gene="recR"
                     /locus_tag="VI319_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY6863148.1"
                     /translation="MRPPSSLEDLIEALRCLPGVGPKSAQRIAYHLLQYDRAGAERLA
                     QTLARAAHAIRRCAKCNSFTETELCALCASDRRDAAQLCVVETPGDLLMMEQTLAYRG
                     LYFVLMGRLSPLDGIGPREIGLDVLHRRAGDGVVKEVIVATNFTNEGEATAHTIGEML
                     KDRGLKITRIARGIPVGGELEYVDTGTLAQAYAERRPVEP"
     gene            complement(2870..3196)
                     /locus_tag="VI319_04550"
     CDS             complement(2870..3196)
                     /locus_tag="VI319_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY6863149.1"
                     /translation="MMKGQLAGLMKQAQQMQDNMKKLQDELGSMQVEGVSGAGMVKVA
                     MTCKHEVKRVTIDPSLLKDDKEMLEDLVAAAMNDAAHKAEAAVQEKMSALTGGLGLPP
                     GLKLPF"
     gene            complement(3210..4733)
                     /gene="dnaX"
                     /locus_tag="VI319_04555"
     CDS             complement(3210..4733)
                     /gene="dnaX"
                     /locus_tag="VI319_04555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY6863150.1"
                     /translation="MSYQVLARKWRPRTFESLVGQEHVVRALTHALDQKRLHHAYLFT
                     GTRGVGKTTLARILAKSLNCETGISSKPCGKCSACLEIDAGRFVDLIEVDAATNTKVD
                     EMRQLLENAVYSPTRGRFKVYVIDEVHMLSTSAFNAMLKTLEEPPEHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPAPAITARLSQILAEEKVPAEDDALRLLAHSARGSMRDAL
                     SLLDQAIAHGGGKVAAAEVRAMLGAIDDEFLLRIAEALAQGDAKAALAAADSMQERNV
                     SFDGALRELAALFHRIALAQTAPAALAEEGAERERVIRLAQALDPEDVQLYYQIAIHG
                     RQDLPFAPDEYAGFTMAVMRMLAFRAEAGEGGRAPAESEGAKPARPSAAPAARAATPT
                     AQGEWPSLVRSLALTGLAGQLAQASELVRFSEGEIELRVPPAAKHLADKTYQDKLRAS
                     LESHFGRPVRLAVKVGETTGNTARDKASASISGDAFVKDLMEHFDATIVESSIKPNR"
     gene            complement(4791..4889)
                     /gene="ffs"
                     /locus_tag="VI319_04560"
     ncRNA           complement(4791..4889)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VI319_04560"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(4904..5734)
                     /locus_tag="VI319_04565"
     CDS             complement(4904..5734)
                     /locus_tag="VI319_04565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6863151.1"
                     /translation="MPIVDSQVHIWAADSPERPWPARHKPHRSDPITAEDLLREMTAA
                     GVDRVVLVPPSWEGERNDVVLAAARAHPQRFAVMGRLDPEAPQSRALLREWRAQPGML
                     GLRFTFHRPSLQPLLTEGRADWVWAEAERHAIPVMLTCPHAILHHLERIAERHPGLRL
                     TLDHLGLLPGTKDEEAFRDFDRLLALARRPNVAVKASALPCYTSDAYPYRRLHPYIER
                     AVAAFGPERVFWGTDLSRLPCTYREAVTMFTDEMPWLGAPEKDEIMGRALCRWLGWLC
                     "
     gene            complement(5742..6077)
                     /locus_tag="VI319_04570"
     CDS             complement(5742..6077)
                     /locus_tag="VI319_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863152.1"
                     /translation="MEPRGNRLACALRTIDGCIGTYDAYPGSAPKSISRVDPVKWDKP
                     PQKDVMEASFSIITDLGMTGAIIRLNQYQWRALNHVKLIEPFYGFMLWGGNPMKVVDD
                     AMMLAKGRI"
     gene            complement(6123..6215)
                     /locus_tag="VI319_04575"
     tRNA            complement(6123..6215)
                     /locus_tag="VI319_04575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6179..6181),aa:Ser,seq:gga)
     gene            6350..7159
                     /locus_tag="VI319_04580"
     CDS             6350..7159
                     /locus_tag="VI319_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6863153.1"
                     /translation="MGSGTFRTSDGCMLAFRVHPAPGPRAPRIALLHSLGLDRSMWDG
                     AVSVLKERAEVLTYDSRGHGRSERRAMPYTPQLLARDLAELLDHTGWTSAAVAGCSLG
                     GCVAQAFAAEYPPRASALGLISTTAWYGAKAPAIWHERAEIAKTQGLRALVDSQLERW
                     FGESFRKLDDAAAAALVDSLLANDVQCYAAACEMLGTLDLRPGLAAITAPTAVIAGKE
                     DQAEPLKMAKALHEGIAGSSLVYIPKSRHLTPVERPKETAEALFDLLEPRR"
     gene            complement(7163..>7491)
                     /locus_tag="VI319_04585"
     CDS             complement(7163..>7491)
                     /locus_tag="VI319_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HEY6863154.1"
                     /translation="KLVAEAAVVGRPDELTGEAIVAFVVLKGLRPSGAEAKKIADELR
                     NWVAKEIGPIAKPKDIRFGDNLPKTRSGKIMRRLLRAIARGEEIKQDVSTLENPAILE
                     QLKQAT"
BASE COUNT         1055 a         2680 c         2575 g         1181 t
ORIGIN      
        1 atcgagaagc gcttcgacat cgcgtgccgg cgcttcgggc tgaacggcca cggcgcgggt
       61 cgcaagccgc cggagctcga ctgcacgaga ttcaagtccc ccgcgcggcg cgacgacgcg
      121 aagcagctcg gcctgttcta acttccctgc ggcgccgggt gcagctcggt cgccttgccc
      181 gccttcacga cctggcccgg cagatcgtgg cggacaatgt cgcccaggcg gcgcgaggcg
      241 gccagcagcc ggtcgagatc gacgccggtc ccgtagccca tcgcctccag catgtgcacc
      301 aggtcttcgg tgcagatgtt ccccgtcgcg cccggcgcaa aggggcagcc gccgagaccg
      361 cccagacagc cttcgtagcg gcgcacgccc gcggcgagac ccgcgagcac gttcgcgagc
      421 cccatgccgc gcgtgttgtg gaaatgcagc gcgaccggca gctcgggaaa ggcttccctc
      481 accgcggacg cgagcgactc gacctggcgc ggatgcgcca tgccggtcgt gtcggcgagc
      541 gtgacggcgt cgacgcccag gtcgacgtag cgccgcacga actccatcac gcgcccggcg
      601 gcctggtcgc cttcgaaggg acagccgaaa gcggtcgaga gcgacccggt cacgtcaatc
      661 cccgttccgt ccagcgcgcg caggatctcg gcgaagcctt cgaacgacgc gtcggtcgaa
      721 cgccgcacgt tcgcccggtt gtgcgtctcc gacaccgaga ccacgaggtt cacctcgtcg
      781 acccgcgccg cggcggcgcg ctcgacgcct ttcgcgttgg cgatcagcgc gacgtagcgc
      841 accgccggat tgcgccggat gcccgccatc acctcctccg cgtcgcgcag gttgggcacc
      901 gccttcggcg agacgaacga ggtcacctcg atcttcgcca cgccggtttc cgagagcgca
      961 tcgagcaact cgatcttgcg ctcggtcggg acgaactgcg gctcgatctg gaagccgtcg
     1021 cgcgcgacga cctcgtggat gtagatgtgc tgcatgcccg cctccccggg cccgtcagac
     1081 ggcacggcgc ccccgcagct gcgcgatctc cgccgcgccg tagccgtgag cggcgagcac
     1141 cgcctcggtg tgctcgccga ggcgcggccc gggccggccg atgtcgccgg gcgtcgccga
     1201 gagcttgggc accacgccgg ggaccttgac gcgcgcacca tcgggcagcg cgatctcgcg
     1261 gatcatgtcg cgcgccaggt actgcgggtc gcgcgcgatg tcggcgatgc tgtagatctt
     1321 gccgctcggg accttcgctt cgttcagcat gcgcaggacg tcctcggcgc cgtgccgcga
     1381 cgtccactcg ccgatcacgg catcgagctc gtccgcgcgc ttcgcgcggc cggcgttgtc
     1441 ggcgagcgcg gggtcttcgg cgaggtcggg ccgcccgatc aggttcatca ggcgcttgaa
     1501 gatgctgtcc gcgttcgcgc cgatcacgac gtgccggcct tcgcgggtca gataggtgtt
     1561 cgacggcacg atgcccgtga gattgctgcc ggtgcgctcg cgcacctcgc cgaacaggtc
     1621 gtactccggc agcgtcgact ccatcatgtt gaacaccgcc tcgtacagcg cgacgtccac
     1681 cacctggcct ttaccggacc tgaggccgcc cgcgtcgcgg tggcgcagcg cggcgagcgt
     1741 gccgatcacg ccgtgcagcg ccgcgagcgc gtcgccgatc gagaggttcg gccgcaccgg
     1801 cggccggtcg ggaaagcccg tgacgtagcg aagtccgccc atcgattcgc cgatcgcacc
     1861 gaagccggcc tggtcgcggt acggcccggt ctggccgaag cccgagagcc gcagcatcac
     1921 gaggccgggg ttcccgtcgg cgagccgctc gtagccgagc ccccatttct ccatcgtccc
     1981 ggggcggaag ttctcgatca cgacgtcggc gccgcgggcg aggcgccgca cgacctcctg
     2041 tccctcgggc aggcgcagat ccgcggtgac cgacttcttg ttccggttct ggacgtacca
     2101 ccacagcgac gtgccctcgt ggagcttgcg ccacttgcga agcggatcgc cttcgggcgg
     2161 ctcaatcttg atcacctcgg cgccgagctc ggcgaagaac ttgcccgcga acggcccggc
     2221 gatgagctgg ccgagctcga tcaccttgag gccggcgagc gcccccggcg tcatggctcg
     2281 accggccggc gctcggcgta cgcctgcgcg agcgtcccgg tgtcgacgta ctcgagctcg
     2341 ccgccgaccg ggatcccgcg cgcgatccgg gtgatcttca gcccgcggtc cttcagcatc
     2401 tcgccgatgg tgtgcgcggt cgcctcgcct tcgttggtga aattcgtcgc gacgatcact
     2461 tccttcacca cgccgtcgcc ggcccggcgg tgcagcacgt cgagcccgat ctcgcgcggc
     2521 ccgatgccgt cgagcggcga cagccgcccc atgagcacga agtacaggcc gcggtacgcg
     2581 agcgtctgct ccatcatcag caggtcgccc ggcgtctcga cgacgcagag ctgcgccgcg
     2641 tcgcggcggt ccgacgcgca cagcgcgcac agctcggtct cggtgaagct gttgcacttg
     2701 gcgcagcgcc ggatcgcgtg cgccgcgcgc gcgagcgtct gcgcgaggcg ctcggcgcca
     2761 gcgcggtcgt actgcaggag gtgatacgcg atgcgctgcg ccgacttcgg cccgaccccc
     2821 ggcaggcagc gcagggcctc gatcaggtct tcgagcgagg acggcggtct caaaacggca
     2881 gcttcagtcc cggcggcagc ccgagtccgc cggtgagggc cgacatcttc tcctggacgg
     2941 cggcctcggc cttgtgcgcg gcgtcgttca tggccgcggc gaccaggtcc tcgagcatct
     3001 ccttgtcgtc cttgagaagg gaaggatcga tcgtcacgcg cttcacctcg tgcttgcagg
     3061 tcatcgcgac cttcaccatg cccgcccccg acacgccctc gacctgcatc gagcccagct
     3121 cgtcctggag cttcttcatg ttgtcctgca tctgctgcgc ctgcttcatc agcccggcga
     3181 gctggccttt catcatgtcc gttctccgtt catcgattcg gcttgatgga cgattcgaca
     3241 atggtcgcgt cgaaatgctc catcaggtcc ttcacgaacg cgtccccgct gatcgacgcg
     3301 ctcgccttgt cgcgcgcggt gttgcccgtg gtctctccca ccttcacggc gaggcgcacc
     3361 ggccggccga agtgggactc gagcgacgcg cgcagcttgt cctggtaggt cttgtccgcg
     3421 aggtgcttcg ccgcgggcgg cacccgcagc tcgatctcgc cttcggagaa gcgcaccagc
     3481 tcgctcgcct gcgcgagctg gcccgcaagc ccggtgagcg cgagcgagcg cacgaggctc
     3541 ggccactcgc cctgcgccgt cggcgtcgcg gcgcgcgcag ccggcgcggc ggacgggcgc
     3601 gcgggcttcg cgccctcgct ttccgcggga gcgcggccgc cctcgcccgc ttccgcgcgg
     3661 aacgcgagca ttcgcatcac cgccatggtg aatcccgcgt actcgtcggg cgcgaacggc
     3721 aggtcttgcc ggccgtggat cgcgatctgg tagtagagct ggacgtcctc ggggtcgagc
     3781 gcctgcgcga gcctgatcac ccgctcgcgc tcggcgcctt cctcggcgag cgccgccggc
     3841 gccgtctgcg cgagcgcgat gcggtggaac agcgccgcca gctcgcgcag cgcgccgtcg
     3901 aaggagacgt tgcgctcctg catcgagtcc gcggcggcga gcgcggcctt cgcgtcgccc
     3961 tgcgcgagcg cctcggcgat ccggagcagg aactcgtcgt cgatcgcgcc gagcatcgcg
     4021 cgcacctcgg ccgcggccac cttgccgccg ccgtgcgcga tcgcctggtc gaggagcgac
     4081 agcgcgtcgc gcatgctgcc gcgcgcggaa tgcgcgagca gccgcagcgc gtcgtcctcc
     4141 gccggcacct tctcttcggc gaggatctgc gagagccgcg ccgtgatcgc cggcgccggc
     4201 atctgcttca ggttgaactg gaggcagcgc gacagcaccg tcaccgggat cttctgcgga
     4261 tcggtcgtcg cgaggatgaa cttgacgtgc tcgggcggct cctcgagcgt cttcagcatc
     4321 gcgttgaacg ccgacgtcga gagcatgtgg acttcgtcga tgacgtagac cttgaagcgg
     4381 ccgcgcgtcg gcgagtacac ggcgttctcg agcaactgcc gcatctcgtc gaccttggtg
     4441 ttggtcgcgg cgtcgacctc gatcaggtcg acgaagcggc ccgcgtcgat ctcgaggcag
     4501 gccgagcact tgccgcaggg cttcgacgag atcccggtct cgcagttgag cgacttcgcc
     4561 aggatgcgcg cgagcgtggt cttgccgacg ccgcgcgtgc ccgtgaacag gtaggcatgg
     4621 tgcagccgct tctggtcgag cgcgtgcgtg agggcgcgca cgacgtgctc ctgcccgacc
     4681 agcgactcga acgtgcgggg acgccacttg cgggcgagga cttgatacga catgccccga
     4741 ttttagcagc cgggaaacga tcgggcctcg cggcgccgat ccggaatggg ggtggcgagc
     4801 cttgcccccg gcacttgcag tgaatagctg tggctgcttc cttccggacc tgaccaggtt
     4861 cactacgctg caatgcgggg aggcccgccg tggtaagagc agtctagcac agccacccga
     4921 gccaccggca cagcgcgcgg cccatgatct cgtccttttc gggcgcgccg agccacggca
     4981 tctcgtcggt gaacatcgtg accgcctctc ggtaggtgca cggcagtcgc gagagatcgg
     5041 tgccccagaa cacgcgctcg ggtccgaacg cggcgaccgc gcgctcgatg tacggatgca
     5101 gccggcgata cggataggcg tcgctcgtgt agcacggcag cgcgctcgcc ttcaccgcga
     5161 cgttgggacg gcgcgcgagc gcgagcagcc ggtcgaagtc gcggaacgcc tcctcgtcct
     5221 tcgtgccggg cagcaggccg agatggtcga gcgtgaggcg cagcccggga tggcgctcgg
     5281 cgatgcgctc caggtgatgc aggatcgcat gcgggcacgt gagcatgacc gggatcgcgt
     5341 gccgctccgc ctccgcccag acccagtcgg cgcgtccctc ggtgagcaac ggctgcagcg
     5401 agggacgatg gaacgtgaag cgcaagccca gcatcccggg ctgcgcgcgc cattcgcgca
     5461 gcaacgcgcg cgactgcggc gcctcgggat ccagccgtcc catcacggcg aagcgctgcg
     5521 gatgcgcgcg cgccgccgcg agcacgacgt cgttgcgctc gccttcccac gaaggcggca
     5581 cgaggacgac gcggtccacg cccgccgccg tcatctcgcg cagcaagtcc tccgcggtga
     5641 tcggatcgga gcggtgcggc ttgtggcgcg cgggccacgg ccgttcgggc gaatcggcag
     5701 cccagatgtg gacctgcgag tcgacgatcg gcacgaccag gctagatcct gcctttcgcg
     5761 agcatcatcg cgtcgtcgac gaccttcatc gggttcccgc cccacagcat gaagccgtag
     5821 aacggctcga tgagcttcac gtggttgagc gcgcgccact ggtattgatt gaggcggatg
     5881 atggcgccgg tcatgccgag atcggtgatg atgctgaagg aagcctccat gacgtccttc
     5941 tgcggcggct tgtcccactt caccggatcg acgcgcgaga tggacttcgg cgcggagccc
     6001 gggtatgcgt cgtaagtccc gatgcagccg tcgatcgtgc gcagcgcgca ggcgagccgg
     6061 ttgccgcgcg gttccatgcg gttctccttc gatcgaaagc cggtcggcga attctaacgg
     6121 cgtggcggag agggtgggat tcgaacccac ggtacggctt ttgaccgtac acgcgctttc
     6181 caggcgcgca ccttcgaccg ctcggtcacc tctccgtccg gaacgccccg cgtcccgcca
     6241 tcgattctac cgtaaggcac ccggccgtct tcgcgccggc cggcggctag tgatacagtt
     6301 acgccggcct tgcagcaaca gcagcgaggg gccgtcgagg agggcgcgcg tgggaagcgg
     6361 aacgttcagg acgtcggacg gctgcatgct cgcgttcagg gtgcatcccg cgcccggccc
     6421 gcgcgcgccg cgcatcgcgc tgctccattc cctcgggctc gaccgcagca tgtgggacgg
     6481 cgcggtgtcc gtgctcaagg agcgcgccga ggttctcacg tacgacagcc gcgggcacgg
     6541 ccgctcggag cgccgcgcga tgccgtatac cccgcagttg ctcgcgcgcg atctcgccga
     6601 gctgctcgac cacaccggtt ggacgtcggc ggcggtcgcg ggatgctcgc tcgggggctg
     6661 cgtcgcgcag gcgttcgccg cggaatatcc gccgcgcgcg tcggcgctcg gattgatctc
     6721 gacgaccgcg tggtacggcg cgaaggcgcc cgcgatctgg cacgagcgcg ccgagatcgc
     6781 gaagacccag ggcctgcgcg cgctggtcga ctcgcagctc gagcgctggt tcggcgagtc
     6841 cttccgcaag ctcgacgacg ccgcggccgc ggcgctggtc gactcgctgc tcgcgaacga
     6901 cgtgcagtgc tacgccgccg cgtgcgagat gctcggcacg ctcgatctcc ggcccggcct
     6961 cgcggcgatc acggcgccga ccgcggtgat cgccgggaag gaggaccagg ccgagccgct
     7021 gaagatggcg aaggcgctgc acgagggcat cgccggatcc agcctcgtct acatcccgaa
     7081 gtcgcggcac ctcactccgg tcgagcggcc gaaggaaacc gcggaggcgc tgttcgatct
     7141 cctcgagccg cgccgctgag cgctaggtcg cctgcttcag ctgctcgagg atcgcggggt
     7201 tctcgagcgt cgagacatcc tgcttgatct cctcgccgcg cgcgatcgcg cgcagcagcc
     7261 gccgcatgat cttgcccgag cgagtcttcg gcaggttgtc gccgaagcgg atgtccttcg
     7321 gcttcgcgat cggcccgatc tccttcgcga cccagttgcg cagctcgtcg gcgatcttct
     7381 tcgcctcggc ccccgaaggc cgcagcccct tgagcacgac gaaggcgacg atcgcctcgc
     7441 cggtgagctc gtccgggcgc cccaccaccg cggcctcggc gaccagcttc g
//
LOCUS       DATDMW010000104         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2270116, whole genome shotgun sequence.
ACCESSION   DATDMW010000104 DATDMW010000000
VERSION     DATDMW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270116"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VI319_04590"
     CDS             complement(<1..422)
                     /locus_tag="VI319_04590"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEY6863155.1"
                     /translation="MRKAEVVRNTKETQIRVRLDLDGRGAARLATGLPFLEHMLDQVA
                     RHGMLDLEIEAKGDLHIDGHHTVEDIGITLGQAFAKALGDKSGIRRFGHAYVPLDEAL
                     SRVVIDFSGRPGLEYHVEYARGLIGDFDVDLVHEFFQG"
     gene            complement(415..1473)
                     /gene="hisC"
                     /locus_tag="VI319_04595"
     CDS             complement(415..1473)
                     /gene="hisC"
                     /locus_tag="VI319_04595"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY6863156.1"
                     /translation="MNPERWVRPEVLAMSAYHVPDARGMVKLDAMENPYSLPEELRRR
                     LAERLARVDLNRYPEPNPRALRELIARTMGVPAGMEVLLGNGSDDLIQVIALALARPG
                     AAMMFPSPTFVMYSVNATLTGMRAVAVPLREDFSLDRDAFVARVKELRPAVVFLAYPN
                     NPTGVLYPPGDVEAIVRAAEGLVVVDEAYHAFAGASFLPRLAEFPNLVVLRTLSKVGL
                     AGLRLGYLAGAPEWLEQFNKVRQVYNVDVLTEAAATFALEHLDVLDAQAARIRADRTE
                     LARGLASLGVTVFPSSANFLLVRLADAPRAFEALRRQGVLVKNLHPGVPGCLRITVGT
                     PDENRILLSALKEALVHA"
     gene            complement(1470..>2069)
                     /locus_tag="VI319_04600"
     CDS             complement(1470..>2069)
                     /locus_tag="VI319_04600"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY6863157.1"
                     /translation="PSEILVICDAGTPAAWVAMDLFSQAEHDEAAQAIVLSPDSAHLD
                     AIAAALEARLAEMPRREIIATSLQTRGALIETRDLAEACAIADRIAPEHLELAVREPR
                     EWLPRLHNAGAIFLGRWSSEAIGDYCAGPNHVLPTAGTARFSSPLGVYDFQKRTSVIE
                     VSRAGAERLGRVAAILADEEGLQAHARSARLRFESGSEP"
BASE COUNT          322 a          724 c          735 g          288 t
ORIGIN      
        1 aaaccctgga agaactcgtg cacgagatcg acatcgaagt cgccgatcag cccgcgcgcg
       61 tactcgacgt gatattcgag cccggggcgt ccggagaaat cgatcaccac gcgcgacagc
      121 gcctcgtcga gcgggacgta ggcatggccg aagcggcgga tgccggactt gtcgccgagc
      181 gccttcgcga acgcctggcc gagcgtgatg ccgatgtcct cgacggtgtg gtggccgtcg
      241 atgtgcaggt cacccttcgc ctcgatctcg aggtcgagca tgccgtggcg cgccacctgg
      301 tcgagcatgt gctcgaggaa cggcaggccg gtcgcgagcc gcgccgcgcc gcggccgtcg
      361 aggtcgagcc gcacgcggat ctgcgtctcc ttggtattgc gcaccacttc ggccttacgc
      421 atggacgagc gcttccttca gggcggaaag cagtatacgg ttttcgtccg gcgtgccgac
      481 cgtgatgcgc aggcagcccg gcacgccggg gtgcaggttc ttcaccagca cgccctggcg
      541 gcgcagcgcc tcgaaggcgc gcggcgcgtc ggccaggcga acgagcagga agttcgcgct
      601 cgacgggaac accgtgacgc cgagcgacgc caggcctcgc gcgagctcgg tgcgatcggc
      661 gcggatgcgc gcggcctgcg cgtcgagcac gtcgagatgc tcgagcgcga acgtcgccgc
      721 cgcctcggtg agcacgtcga cgttgtagac ctgccgcacc ttgttgaact gctccagcca
      781 ttccggcgca ccggcgagat agccgagccg caggcccgcg aggcccactt tcgacagcgt
      841 gcgcagcacg acgaggttcg ggaactcggc gagccgcggc aggaagctcg cgccggcgaa
      901 ggcatggtag gcctcgtcca ccacgaccag cccttcggcg gcgcgcacga tcgcttccac
      961 gtcgcccggc gggtagagca cgccggtcgg attgttcgga taggcgagga acacgaccgc
     1021 cgggcgcagc tccttcacgc gcgcgacgaa cgcgtcgcgg tcgagcgaga agtcttcgcg
     1081 cagcggcacc gccacagcgc gcatgccggt gagcgtcgcg ttgaccgagt acatgacgaa
     1141 ggtcggcgac gggaacatca tcgcggcgcc gggccgcgcg agcgcaaggg caatgacctg
     1201 gatcagatca tccgagccgt tgccgaggag cacctccatc cccgccggca cgcccatcgt
     1261 gcgcgcgatg agctcgcgca gcgcgcgcgg gttgggctcc ggatagcgat tgagatccac
     1321 gcgcgccagc cgctcggcga gccgccggcg cagctcctcg ggcagcgaat acgggttctc
     1381 catggcgtcg agcttcacca tgccgcgcgc gtcgggcacg tgataggcgg acatggccag
     1441 cacttccggc ctgacccagc gttccggatt cacggttcgg acccgctttc gaaccgaagg
     1501 cgggccgagc gcgcgtgcgc ctgcaagcct tcctcgtcgg cgaggatcgc cgccacccgg
     1561 cccaggcgct cggcacccgc gcgcgacacc tcgatgacgc tcgtgcgctt ctggaagtcg
     1621 tacacgccga gcggcgacga gaagcgcgcg gtgcccgcgg tcggcagcac gtggttcggg
     1681 ccggcgcaat agtcgccgat cgcctccgag ctccagcgcc cgagaaagat ggcgcccgcg
     1741 ttgtgcagcc gcggcagcca ttcgcgcggc tcgcgcaccg cgagctcgag gtgctcgggc
     1801 gcgatgcgat cggcgatggc gcacgcctcg gcgaggtcgc gtgtctcgat cagcgcgccg
     1861 cgcgtctgga gcgatgtggc gatgatctcg cgccgcggca tctcggcgag gcgcgcctcg
     1921 agcgccgccg cgatcgcgtc gaggtgcgca ctgtcgggcg aaagcacgat cgcctgcgcg
     1981 gcctcgtcgt gctccgcctg cgagaagagg tccatcgcca cccacgccgc gggcgtgccc
     2041 gcgtcgcaga tcaccaggat ctccgaggg
//
LOCUS       DATDMW010000105         5814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1398744, whole genome shotgun sequence.
ACCESSION   DATDMW010000105 DATDMW010000000
VERSION     DATDMW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398744"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1040)
                     /locus_tag="VI319_04605"
     CDS             complement(75..1040)
                     /locus_tag="VI319_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6863158.1"
                     /translation="MKLALRAAACGIAFSLAGACLAQDAQWPGRPVRMIVPFAPAGPT
                     DIIARLVVSKAGEILVTQVVIENHAGAGGNIGAAMVAKSAPDGYTTLLTSSAFAVNVS
                     LFSSPGYETRQFEPVVVAARQPNLIFVAASYPPHTMAELVDLAKRTRPGYASPGVGTT
                     PHLTAETVFRSMAGLDLTAIHFKGAGQAVTAVIAGEPPIGCGAISGPLPYIKSGKLRP
                     IAVSSDKRVATLPDVPTLAEAGFPGVEDYTWVGIFFPAGTPHTIVQKLNDAVNRALHD
                     AAIRQRLDQLAFDPVGGSPQEFADYLKQEIPKWAKVVRAAKVKVD"
     gene            1111..2082
                     /locus_tag="VI319_04610"
     CDS             1111..2082
                     /locus_tag="VI319_04610"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY6863159.1"
                     /translation="MAARKLTRQDLRDAAEKYKNWGKWGPKDEIGTLNYTTPEDIVAA
                     ARLVRKGKVISLALNFDHTGPQGAKSKYPAMGRTNPIHTMLRTGTDAYSGVLDHRGIR
                     AADDMVTMPLQCGTQWDGLGHVFYENSMWNGYDCREVTSAGAQKCGIEKTKARMVGRG
                     VFLDVPRALGKKVLDDGYAITCADLDRTAKAQNVEVRRGDFVVVRTGQMEAKLEAGSW
                     DGYPGGDAPGFGFETLEWIQRTQLAALASDTWGCEVRPNETEPGINQPWHWITIPIMG
                     MTMGEIFYLKDLADDCAADRVYEFLFVAPAIPITGAVGSPTNPLAIK"
     gene            complement(2086..2622)
                     /locus_tag="VI319_04615"
     CDS             complement(2086..2622)
                     /locus_tag="VI319_04615"
                     /inference="COORDINATES: protein motif:HMM:NF020931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HEY6863160.1"
                     /translation="MASFDAFFPTLLRNEGGFVDDPVDPGGATNKGVTLETFKSSAQR
                     YLGIEPTLENLKALTDDQAAKIYKPLYWDKIRGDDIQLQDLADIVFDFQVNAGAAASK
                     LLQRVLNDLGAQPPLGVDGEIGPGTMAALARADAVAVYRAYKQGRIAYYQDLVAKRPP
                     LGKFLNGWLKRVNAFPEL"
     gene            complement(2635..3012)
                     /locus_tag="VI319_04620"
     CDS             complement(2635..3012)
                     /locus_tag="VI319_04620"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEY6863161.1"
                     /translation="MTASRVLAIVVLAAILGAPARAPAQPACENARTTAAMRQCEIAR
                     LKQAEVGMDAAYQALAARLDAAGRAKLSAAQDAWRAYRAAEAEFQADHAREGTLAPLI
                     ADSVRADLTEARRRELERALKSK"
     gene            complement(3009..3332)
                     /locus_tag="VI319_04625"
     CDS             complement(3009..3332)
                     /locus_tag="VI319_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863162.1"
                     /translation="MKRTVAGVAAAALCGCTTMVVPEVKEALKCEPSDAMLAACGSPS
                     SIKDGVTFGEMIEVSGRDRDALKECALKQQALAQSIAACNERIEKYNAAIREMNARNR
                     DAAKQ"
     gene            complement(3344..3847)
                     /locus_tag="VI319_04630"
     CDS             complement(3344..3847)
                     /locus_tag="VI319_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863163.1"
                     /translation="MLDKVKLVIAGVIGAVLFGGGYSFGARKVDALEAQIEELKKTGD
                     SAEARRKQAQADIDKALKDQAAEHAKAMEALKAESGRREKDLGDALAGANARNTDLQS
                     KLKANEAQRAKLQAAMATASDAQRRTLQGQLQVLSAEDTKLLAQLDANKCLALAVPTT
                     VIGPLVK"
     gene            4030..5706
                     /locus_tag="VI319_04635"
     CDS             4030..5706
                     /locus_tag="VI319_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY6863164.1"
                     /translation="MAKMTGAQCLAEMLKGYGVTHVFMVPAVLRRTFAEMEKRTGIAR
                     IHTHGEKSAAYMADGYARASGRPGICMAQVIGALNLAAGLRDAYLAHSPVIAFTGGRD
                     PKTKFRKVYQEVDDVPAFEPVTKLNATVDDVSRFPDMVRQAFRVAVSGAPGPVHLQFR
                     GNEGQIDAEEADMEPLVEPLFSRVPPFRPEPDREQMRAALVLLQQAERPVIVAGGGVR
                     ASGGARALTALAERLQIPVATSLNGKDSIPGTHPLSVGVVGTYSRESANRVVAQADLV
                     CFVGTEAGGMTTHFWAVPKIGVKAVQIDIEPEALGRNYPLQAAVLGDARTTLERMLEA
                     ADASSAAKRKAWIATARGMCAEWREKYTSQLESSAVPMRPERLVAELTQRMPDDATVI
                     VDTGHAGMWMGGMFDLRTPKQNYMRSAGHLGWAFPAGLGAKCATPERPVVVFTGDAGL
                     WYHIGELETAVRWGINTVVVVNNNGGGNQSKRGFDRAYGGEQTQKARELWTYRMVSFA
                     KIAEDIGALGIRVEKPNDIGPAIERAVAAKRPAVVDVVTDIEALAPTAVG"
BASE COUNT          870 a         2017 c         2048 g          879 t
ORIGIN      
        1 cgacgcgatg gagctcggct cgctcggctc ggtcagcttc atcctctgag ccgctcaggt
       61 cggtccagcc gggctcagtc caccttgacc ttggccgctc gcaccacctt cgcccacttc
      121 gggatctcct gcttcaggta gtcggcgaac tcctgcggcg atccgcccac gggatcgaag
      181 gcgagctggt ccaggcgctg gcggatggcg gcgtcgtgca gcgcgcggtt gacggcgtcg
      241 ttcagcttct gcacgatcgt gtgcggcgtg cccgccggga agaagatgcc cacccaggtg
      301 tagtcctcga cgccgggaaa gcccgcctcg gcgagcgtcg gcacgtcggg aagcgtggcc
      361 acgcgcttgt cgctcgagac cgcgatcggg cgcagcttgc cggacttgat gtacggcagc
      421 ggccccgaga tcgctccgca gccgatgggc ggctcgcccg cgatgaccgc ggtgacggcc
      481 tgccccgcgc ccttgaagtg gatcgcggtc aggtcgagcc ccgccatcga gcggaacacg
      541 gtttccgcgg tcaggtgcgg cgtagtgccg acgccgggag acgcgtagcc cgggcgcgtg
      601 cgcttcgcga ggtcgacgag ctcggccatg gtgtgcggcg gatagctcgc cgcgacgaag
      661 atcaggttcg gctgccgggc agcgacgacc acgggctcga actggcgcgt ctcgtagccc
      721 ggactggaga acaggctgac gttgaccgcg aacgcggagg aggtcagcag cgtggtgtag
      781 ccgtcgggcg ccgatttcgc caccatcgcc gcgccgatgt tgccgccggc gccggcgtgg
      841 ttctcgatca cgacctgcgt gaccagtatc tctcccgcct tggagacgac caggcgcgcg
      901 atgatgtcgg tcggtcccgc aggggcgaac ggcacgatca tgcgcacggg ccggccgggc
      961 cattgcgcat cctgcgcgag gcacgcgccg gcgagcgaga aagcgatgcc gcacgcggcg
     1021 gcgcgtaagg cgagcttcat gggtccctcc tccgggatgc cgcaatggta gcatccgggc
     1081 tcgatcgcat cacaccaccc cgaggagacc atggccgccc gcaagctcac ccgtcaggac
     1141 ctccgcgacg ccgcggagaa atacaagaac tggggcaagt gggggcccaa ggacgagatc
     1201 ggcacgctga actacacgac gcccgaggac atcgtcgccg cggcgcgcct cgtgaggaaa
     1261 ggcaaggtga tctcgctcgc gctcaacttc gatcacaccg ggccgcaggg cgcgaagagc
     1321 aagtaccctg cgatggggcg caccaacccg atccacacga tgctccggac cggcaccgac
     1381 gcgtattcgg gcgtgctcga ccaccgcggc atccgcgccg cggacgacat ggtgacgatg
     1441 ccgctccagt gcggcacgca gtgggacggt ctcgggcacg tcttctacga gaactcgatg
     1501 tggaacggct acgactgccg cgaggtcacg tccgccggcg cgcagaagtg cggcatcgag
     1561 aagaccaagg cgaggatggt cggccgcggc gtcttcctcg acgtgccgcg cgcgctcggg
     1621 aagaaggtcc tcgacgacgg ctacgcgatc acctgcgccg acctcgatcg cacggcgaag
     1681 gcgcagaacg tcgaagtgcg ccgcggggac ttcgtggtcg tgcgcaccgg gcagatggag
     1741 gccaagctcg aggccggcag ctgggacggc tatcccggcg gcgacgcgcc cggcttcggc
     1801 ttcgagacgc tcgaatggat ccagcgcacg cagctcgcgg cgctcgcctc cgacacctgg
     1861 ggctgcgagg tgcgtcccaa cgagaccgag cccggcatca accagccctg gcactggatc
     1921 accataccca tcatgggcat gaccatgggc gagatcttct acctgaagga tctggccgac
     1981 gactgcgccg ccgaccgcgt ctacgagttc ctgttcgtcg cgccggcgat cccgatcacg
     2041 ggcgcggtcg gctcgccgac gaatccgctc gcgatcaagt gacgcctaaa gctccgggaa
     2101 cgcgttcacg cgcttgagcc acccgttcag gaacttcccg agcggcggac gcttggcgac
     2161 cagatcctgg tagtacgcga tccggccctg cttgtacgcc cggtagacgg cgaccgcgtc
     2221 ggcgcgagcg agcgcggcca tcgtgccggg gccgatctcg ccgtcgacgc cgagcggcgg
     2281 ctgcgcgccc aggtcgttga gcacgcgctg cagcagctta gacgccgccg cgcccgcgtt
     2341 cacctggaag tcgaacacga tgtcggcgag atcctggagc tggatgtcgt cgccgcggat
     2401 cttgtcccag tacagcggct tgtagatctt cgcggcctgg tcgtcggtga gcgccttcag
     2461 gttctcgagc gtgggctcga tcccgaggta gcgctgcgcg gacgatttga aggtttcgag
     2521 cgtcacaccc ttgttggtcg cgccgccggg gtcgaccgga tcgtcgacga agccgccttc
     2581 gttccggagc agcgtcggga agaacgcgtc gaagctcgcc atgtcgcctc ctcgttattt
     2641 cgacttgagc gcccgctcga gctcgcggcg ccgcgcctcg gtcagatcgg cgcgcacgct
     2701 gtccgcgatc agcggcgcga gcgtgccctc gcgcgcgtgg tcggcctgga actcggcttc
     2761 cgcggcgcgg tacgcgcgcc acgcgtcctg cgcggcgctc agcttcgccc ggcccgccgc
     2821 gtcgagacga gcggcgagcg cctggtacgc cgcgtccatc cccacctccg cctgcttcag
     2881 ccgcgcgatc tcgcactggc gcatcgcggc ggtggtgcgc gcgttctcgc acgcgggctg
     2941 cgcgggagca cgtgccggcg cgccgaggat cgccgcgagg accacgatgg cgaggacccg
     3001 cgacgccgtc actgcttcgc ggcgtcccgg ttccgggcgt tcatctcgcg gatcgcggcg
     3061 ttgtacttct cgatgcgctc gttgcaggcg gcaatcgact gcgccagcgc ctgctgcttg
     3121 agcgcgcatt ccttgagcgc atcgcggtcg cgccccgaga cctcgatcat ctcgccgaag
     3181 gtcacgccgt ccttgatcga cgacggcgac ccgcaggcgg cgagcatcgc gtcggagggc
     3241 tcgcacttga gcgcttcctt gacctcggga acgaccatgg tggtgcagcc gcacaaggcg
     3301 gcggcggcga cgccggcaac ggtccgcttc atgaggtctc ctctcatttg acgagcggcc
     3361 cgatgaccgt ggtgggaacg gcgagggcga ggcacttgtt cgcgtcgagc tgcgcgagca
     3421 gcttggtgtc ctcggcgctc agcacctgga gctggccttg cagcgtgcgg cgctgcgcgt
     3481 cggatgcggt cgccatggcg gcctggagct tggcgcgctg cgcctcgttc gccttgagct
     3541 tcgactgcag gtcggtattg cgcgcattgg cgccggcgag cgcgtcgccg aggtccttct
     3601 ctcggcggcc cgattccgcc ttcagcgcct ccatcgcctt ggcgtgctcg gccgcctggt
     3661 ccttcagcgc cttgtcgatg tcggcctgcg cctgcttgcg ccgcgcctcg gcgctgtcgc
     3721 cggtcttctt cagctcctcg atctgcgcct cgagggcatc gaccttgcgg gcgccgaagc
     3781 tgtatccgcc cccgaacagc accgccccga tcacgccggc gatgaccagc ttgaccttgt
     3841 ccagcatggc tcccccctat tgtcggccgc gtagccccgc atcctagcgc acaaagccgg
     3901 ggcgcatgtc ggcgtcatgc gcgtcctaca gcggccgtcg acgccggctg acacggccgc
     3961 gccatggaac cggcgctgcg gctaaaatcg gcccggaccc gcggggtcga tgtccgaacg
     4021 aggagaagca tggccaagat gaccggggcg caatgcctcg cagagatgct gaagggctac
     4081 ggcgtgacgc acgtgttcat ggtccccgcg gtgctgcgcc gcacgttcgc cgagatggag
     4141 aagcgcaccg gcatcgcgcg catccatacc cacggagaga agtccgcggc ctacatggcc
     4201 gatggctacg cgcgcgcctc gggaaggccc ggcatctgca tggcccaggt gatcggcgcg
     4261 ctcaatctcg cggccggcct gcgcgacgcg tacctcgcgc attcgccggt gatcgctttc
     4321 accggagggc gcgacccgaa gaccaagttc cgcaaggtct accaggaggt cgacgacgtg
     4381 ccggcgttcg agccggtcac gaagctgaac gcgacggtgg acgacgtctc gcgcttcccc
     4441 gacatggtgc gccaggcgtt ccgcgtggcg gtctcgggcg cgccgggccc cgtgcacctg
     4501 cagttccgcg gcaacgaggg ccagatcgac gccgaggagg ccgacatgga gccgctggtc
     4561 gagccgctct tctcgcgcgt gccgcccttc cggcccgagc ccgaccgcga gcagatgcgg
     4621 gcggcgctcg tgctgctgca gcaggccgag cgccccgtga tcgtcgcggg cggcggcgtg
     4681 cgcgcgtccg gcggcgcgcg cgcgctgacc gcgctcgccg agcgtctgca gattccggtg
     4741 gcgacctcgc tcaacgggaa ggattcgatc cccggcacgc atccgctctc ggtcggcgtc
     4801 gtcggcacct actcgcgcga gagcgcgaac cgggtcgtgg cgcaggccga tctcgtgtgc
     4861 ttcgtcggca ccgaggcggg cgggatgacg acgcacttct gggccgtgcc gaagatcggc
     4921 gtgaaggcgg tccagatcga catcgagccc gaggccctcg ggcgcaacta cccgcttcag
     4981 gccgcagtcc tgggcgacgc gcgcacgacg ctcgagcgca tgctcgaggc ggcggacgct
     5041 tcgagcgccg cgaagcgcaa ggcctggatc gcgaccgcgc gcggcatgtg cgccgagtgg
     5101 cgcgagaagt acacctcgca gctcgagtcg agcgcggtgc ccatgcgccc cgagcgtctc
     5161 gtcgccgagc tcacgcagcg catgcccgac gacgcgaccg tgatcgtcga caccgggcac
     5221 gccggcatgt ggatgggcgg gatgttcgac ctgcgcacgc cgaagcagaa ttacatgcga
     5281 agcgccggtc acctcggatg ggcgttcccc gcggggctgg gcgcgaaatg cgcgacgccc
     5341 gagcggccgg tcgtggtctt caccggcgac gccggcctct ggtaccacat cggcgagctg
     5401 gagacggccg tgcgctgggg aatcaacacc gtcgtcgtcg tcaacaacaa cggcggcggc
     5461 aaccagtcga agcgcggctt cgaccgcgcc tacggcggcg agcagacgca gaaggcgcgc
     5521 gagctctgga cctaccgcat ggtgagcttc gccaagatcg ccgaggacat cggcgcactc
     5581 gggatccgcg tcgagaagcc gaatgacatc ggcccggcga tcgagcgcgc cgtcgcggcg
     5641 aagcggccgg cggtggtcga cgtcgtcacc gatatcgagg cgctggcgcc gacggcggtc
     5701 ggctgagaaa aaaatgcccg cgcgtggcgc gggcatccga ggcctgctgg tccgtcagaa
     5761 cacgaaccgg gtggtcgtca ggcgcgtcgt cgtggtcgtc gtggtaagcg tcgg
//
LOCUS       DATDMW010000106         5149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2396567, whole genome shotgun sequence.
ACCESSION   DATDMW010000106 DATDMW010000000
VERSION     DATDMW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2396567"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..311
                     /locus_tag="VI319_04640"
     CDS             153..311
                     /locus_tag="VI319_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEY6863165.1"
                     /translation="MTVGTILLIVLVLLLVGAIPAWPYSRAWGYGPSSILGIVVLILL
                     VLALTGRL"
     gene            422..751
                     /locus_tag="VI319_04645"
     CDS             422..751
                     /locus_tag="VI319_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863166.1"
                     /translation="MSEHVWQIFDVDELQRRVAGGKAPAIAEFLRTPSLSCAVYRLPA
                     GARDMQAPHVEDELYFVIRGRAKIRIAGEEHQVEPGRILYVRATSEHSFFDIQEDLTL
                     VAIFGAS"
     gene            complement(770..1630)
                     /locus_tag="VI319_04650"
     CDS             complement(770..1630)
                     /locus_tag="VI319_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY6863167.1"
                     /translation="MRRSFLAAVLAGLAQLALLARAPVASAIDAPPRRAAARVALVEG
                     DARFLDAYGGAERRPRVGDLLYEGDRIVTGPDGEVQLAMEDGGYLGVRPDTEVQVVAF
                     RAEGRPDDLSVIALARGALRAVTGWIAGGGTGGTGVVLRTPNAAIGVRGTDHEALVVP
                     EGARNAEPGTYDRVYSGATVIESRGGELGVGAGQAGFAPLDGRGARVLERIPEVFRPT
                     RNERRFDGLHDRIQRELEKRRQERRSFNDRDQRGDRAERSERAERPERVERVERVEKV
                     DRVERVERLK"
     gene            complement(1692..2795)
                     /locus_tag="VI319_04655"
     CDS             complement(1692..2795)
                     /locus_tag="VI319_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863168.1"
                     /translation="MKTRTAIAVLAAAVLASCSHVPGVRSESDAPRFAVDPYWPRPLP
                     GNWILGQVSGIAVDKNDHIWIVHRPGTLVDDEKGAMQNPPATRCCTPAPPVLEFDAQG
                     NLYRSWGGPGKGYDWPESEHGIYVDPHGDVWIGGNGNKDQQILKFSPDGKFLKQIGRP
                     GPTGGSNNTGALGRPANVEIDAATNELFIADGYGNRRVLVLDADSGKYKRHWGAYGNR
                     PSDDKQPAYDPAQPPSPQFGNPVHCARLSSDGLVYVCDRANDRIQVFEKSGKFVREFR
                     IEPQTLQNGSVWDLVLSRDPQQRWIFMADGANGQVLVIERASGKVAAGFGRSGRMAGE
                     FKWVHNIAIDSKGNLYTSEVGTGRRAQKFVRVD"
     gene            2862..3956
                     /locus_tag="VI319_04660"
     CDS             2862..3956
                     /locus_tag="VI319_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEY6863169.1"
                     /translation="MKPLLWAALALLCGPVYAQSQSVPTIAFDASDPLHLPKDLYLGE
                     ATGVAVNSKGHIFVLSRGNTTGPAYGAAATQLLEFDPQGKFLREIGHNLYAWSFGHTV
                     RVDKSDNLWVTDKGSDMVVKFNPAGRVLMVFGRKQESSDKETGPLEHPKPPLPPQEGR
                     FRQVTDVAFAPDGSVFISDGYINSRVAKVDRNGVWLKSFGEPGNQAGQFSTPHSIAAD
                     AKGNIYVADRGNRRIQVFDGEGNFQREIRIDVPVPAGARTALGPKPDLAKLEASGGPF
                     TQAPGAPWAVCITPGPNQVLYTSDAFPGRVYKLSLDGKVLGVLGESGKQLRQFGWIHE
                     IACPSENVLYVAELLNWRVQKLVLHPSGKR"
     gene            complement(3961..>5149)
                     /locus_tag="VI319_04665"
     CDS             complement(3961..>5149)
                     /locus_tag="VI319_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6863170.1"
                     /translation="IAPSTPVVLQAIYNHSYLNTAALKAAGIDAKTPDPRGGKIEKDA
                     KGRPTGVVSGPGGVAFVAARIPLPDKGAWTENAKRLVAELNAMGLTAWYDAGGRGVDE
                     RHYAVYRELAERGELNARAFWTTIRQPSTPEQVDKVLAEIAQAKPFQGSDHFDNIGWG
                     ESVYLPATTSLLRPGFQAKPEDMAQVRRIALAVAERGMMLNAHVEMTPAIDAYLDVYE
                     AINRERPIKGLRWVLSHLDQVDDAQLERMKKLGLYAGLHSRPLIQGALMHKAHGDRAW
                     DMPPFRRVQASGIVWGLGSDATAVTPSNPFYTLWFAVTGRMIGGRQVNRQTVTREQAL
                     VAHTRSNALFLFQESNLGSIAPGKYADLVVLDRDYLAVPADEIKDIRPVMTLVGGKVV
                     YSR"
BASE COUNT          725 a         1834 c         1764 g          826 t
ORIGIN      
        1 ctgtcgcttg gacctcggtc gcgccccgcg gtgctcggaa gcggccgagt ggttcaacct
       61 gcccagcgcg cgcgccgccg gccgcgccgt tcgagacaca cgccggccct acgcttgccc
      121 gtgcgagagg aacaacggaa ggaatccgaa caatgaccgt gggaacgatt ctcctgatcg
      181 tgctcgtcct gctgctggtc ggcgcgatac cggcgtggcc ctacagccgc gcctggggct
      241 acggcccgag cagcatcctc gggatcgtcg tcctgatcct gctggtgctg gcgctgacgg
      301 gccggctgta gcccggcggc tcgctcgccc gggatttgac gcccgtcaaa tcccgccggc
      361 gcacgccggg cactatcggc gcatcggaac cgccgagggc gccggtcggc gcgggcaaac
      421 catgagcgag catgtctggc agattttcga cgtcgacgag ctgcagcgcc gggtcgcggg
      481 cgggaaggcg ccggcgatcg cagaattcct gcgcacgccg tcgctcagct gcgccgtgta
      541 ccggctgccc gcgggcgcgc gcgacatgca ggcgccgcac gtcgaggacg agctctactt
      601 cgtgatccgt gggcgcgcca agatccgcat cgcgggcgag gagcatcagg tggagccggg
      661 acgcatcctg tacgtgcgcg cgacctccga gcacagcttc ttcgacatcc aggaagatct
      721 gacgctggtc gcgatcttcg gcgcgtcgtg aggcgaccgg gcccgcgcgc tatttgaggc
      781 gctcgactct ctcgacccgg tcgacctttt ccacgcgctc gacccgttcg acgcgctcgg
      841 gccgctccgc gcgctcggac cgctctgcgc gatcgccgcg ctggtcccgg tcgttgaagc
      901 tgcggcgctc ctggcggcgc ttctcgagct cccgctggat gcggtcgtgc agaccgtcga
      961 agcggcgctc gttgcgcgtc ggacggaaca cttcgggaat gcgctcgagc acgcgcgcac
     1021 cgcggccatc gagcggggcg aagccggcct gtccggcccc gacgccgagc tcgccgccgc
     1081 ggctctcgat caccgtcgcg ccggagtaga cacgatcgta agttcccggc tcggcattgc
     1141 gcgctccctc gggcacgacg agcgcctcgt ggtcggtgcc gcgtacgccg atcgccgcgt
     1201 tcggcgtgcg caggaccacg cccgtgccgc ccgtgccgcc ccccgcgatc catcccgtga
     1261 ccgcacgcag cgcgccgcgc gcgagcgcga tgaccgataa atcgtcagga cggccctcgg
     1321 cgcggaacgc gacgacctgg acttccgtgt cgggccgcac gccgagatag ccgccgtcct
     1381 ccatcgcgag ctggacctcg ccgtcggggc cggtgacgat gcggtcgcct tcgtacagga
     1441 ggtcgccgac cctggggcgg cgctcggcgc cgccataagc gtcgagaaag cgcgcgtcgc
     1501 cctcgaccag cgcgacgcgc gcggcggcgc ggcgcggcgg cgcatcgatc gcagatgcga
     1561 ccggcgcgcg cgcgaggagc gcgagctgtg ccaggccggc gagcaccgcc gcaaggaagg
     1621 agcgccgcat gcgtccatca catcacgcgg acgcgggcgc ggcttgattc agatcaaacg
     1681 gcggctcggg ctcagtcgac gcggacgaat ttctgcgcgc ggcgcccggt gccgacctcg
     1741 gaggtataga ggttcccctt ggagtcgatg gcgatgttgt gcacccactt gaactcgccc
     1801 gccatgcggc cgctgcgtcc gaagccggcg gcgaccttgc cgctcgcgcg ctcgatcacg
     1861 agcacctggc cgttcgcgcc gtcggccatg aagatccatc tctgctgcgg atcgcgcgag
     1921 agcacgaggt cccacaccga gccgttctga agcgtctgcg gctcgatccg gaactcgcgc
     1981 acgaacttcc cgctcttctc gaacacctgg atgcgatcgt tcgcgcggtc gcagacgtag
     2041 accagcccgt cgctcgacag ccgcgcgcag tgcaccgggt tgccgaattg cggcgatggc
     2101 ggctgcgccg ggtcgtacgc gggctgcttg tcgtcgctcg ggcggttgcc gtacgcgccc
     2161 cagtggcgct tgtacttgcc gctgtcggcg tcgagcacga gcacgcgccg gttgccgtag
     2221 ccgtcggcga tgaacagctc gttggtggcg gcgtcgatct cgacgttcgc cggccggccg
     2281 agcgcgccgg tgttgttcga gcctcccgtc ggccccggcc tgccgatctg cttcaggaac
     2341 tttccgtccg ggctgaactt caggatctgc tggtccttgt tgccgttgcc cccgatccag
     2401 acgtcgccgt gcgggtcgac gtagatgccg tgctcgctct cgggccagtc gtagcctttg
     2461 ccgggcccgc cccagctgcg gtagaggttg ccctgcgcgt cgaactccag caccggcggc
     2521 gcgggcgtgc agcagcgcgt cgcgggcgga ttctgcatcg cgcccttctc gtcgtcgacg
     2581 agcgtcccgg gccggtgcac gatccagatg tggtcgttct tgtcgaccgc gatgcccgag
     2641 acctgaccga ggatccagtt gcccgggagg ggccttggcc agtacgggtc gaccgcgaag
     2701 cgcggcgcat ccgattccga tctcacgccc ggcacgtggc tgcagctcgc gagaacggcg
     2761 gcggcgagca cggcgattgc ggtgcgggtt ttcatggcgt ctcctccgga tcgacccagc
     2821 cggagtatat tccggccggt ccaaccccga ggaggtctcc catgaaaccc ctgctgtggg
     2881 cagcgctcgc gctgctctgc ggtcccgttt acgcgcaatc gcaatcagtc ccaaccatcg
     2941 cgttcgacgc gagcgacccg ctgcacctgc ccaaggatct ctatctcggc gaggccacgg
     3001 gcgtcgccgt gaactcgaaa ggacacatct tcgtgctctc gcgcggcaac accaccgggc
     3061 ccgcgtacgg cgcggcggcg acgcagctgc tcgagttcga tccgcagggc aagttcctgc
     3121 gcgagatcgg acacaacctc tacgcctggt cgttcggcca cacggtgcgc gtggacaaga
     3181 gcgataacct ctgggtgacc gacaagggct cggacatggt ggtgaagttc aacccggcgg
     3241 gccgcgtgct gatggtgttc ggccgcaagc aggagtcctc cgacaaggag accggtccgc
     3301 tcgagcatcc gaagccgccg ctgcctccgc aggagggccg cttccgccag gtgaccgacg
     3361 tcgccttcgc tcccgacggc agcgtgttca tttccgacgg ctacatcaac tcgcgcgtcg
     3421 ccaaggtcga ccgcaacggc gtgtggctga agtccttcgg cgagcccggc aaccaggccg
     3481 gccaattcag cacgccgcac tcgatcgcgg ccgacgcgaa gggcaacatc tacgtcgccg
     3541 accgcggcaa ccgccgcatc caggtgttcg acggcgaagg caacttccag cgcgagatcc
     3601 gcattgacgt gccggtgccc gcgggcgcgc gcacggcgct cgggcccaag cccgacctgg
     3661 cgaagctcga ggcctcgggc gggccgttca cgcaggcgcc gggcgcgccg tgggcggtgt
     3721 gcatcacgcc cgggccgaac caggtcctct atacctccga cgctttcccg ggccgcgtct
     3781 acaagctgtc gctcgacggc aaggtgctcg gcgtgctcgg cgagtccggg aagcagctca
     3841 ggcaattcgg ctggatccac gagatcgcct gcccgtcgga gaacgtgctc tacgtcgccg
     3901 agctcctcaa ctggcgcgtg cagaagctgg tcctgcaccc cagcggcaag cggtgagcgc
     3961 ctatcgcgag tagacgactt tcccgccgac gagcgtcatc acgggacgga tgtccttgat
     4021 ctcgtcggcg ggcaccgcca ggtagtcgcg gtcgagcacg acgaggtccg cgtactttcc
     4081 gggcgcgatc gacccgaggt tgctctcctg gaagagaaag agcgcgttcg agcgcgtgtg
     4141 cgcgacgagc gcctgctcgc gcgtgacggt ctggcggttc acctggcggc caccgatcat
     4201 gcgcccggtg accgcgaacc atagcgtgta gaacgggttc gacggcgtca ccgcggtcgc
     4261 gtccgacccg aggccccaca cgatcccgct cgcctgcacg cgccggaacg gcggcatgtc
     4321 ccaggcgcga tcgccgtgcg ccttgtgcat gagcgcaccc tggatcagcg gccggctgtg
     4381 caggcccgcg tagaggccga gcttcttcat gcgctcgagc tgcgcatcgt cgacctggtc
     4441 gaggtgcgag agcacccagc gcaggccctt gatcggccgc tcgcggttga tcgcctcgta
     4501 gacgtcgaga taggcgtcga tcgccggcgt catctcgacg tgcgcgttca gcatcatgcc
     4561 gcgctcggcg accgcgagcg cgatgcggcg cacctgcgcc atgtcctcgg gcttcgcctg
     4621 gaagcccggg cgcagcaggc tcgtcgtcgc gggcagatac accgattcgc cccagccgat
     4681 gttgtcgaaa tggtcgctgc cctggaacgg cttcgcctgc gcgatttcgg cgagcacctt
     4741 gtcgacctgc tcgggcgtcg acggctgccg gatcgtggtc cagaacgcgc gcgcgttcag
     4801 ctcgccgcgc tcggccagct cgcggtagac cgcgtagtgg cgctcgtcga cgccgcgtcc
     4861 tccggcgtcg taccaggcgg tcaggcccat cgcgttcagc tcggcgacca ggcgcttcgc
     4921 gttctcggtc cacgcgccct tgtcgggcag cgggatcctc gccgcgacga aggcgacgcc
     4981 gcccggcccg ctcaccacgc cggtcggccg gcctttggca tccttctcga tctttccgcc
     5041 gcgcgggtcg ggcgtcttcg cgtcgatccc cgcggctttg agcgccgcgg tgttgagata
     5101 gctgtggttg tagatcgcct gcagcaccac cggcgtcgac ggcgcgatg
//
LOCUS       DATDMW010000107        29437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1649324, whole genome shotgun sequence.
ACCESSION   DATDMW010000107 DATDMW010000000
VERSION     DATDMW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 29437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29437
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649324"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..2000
                     /locus_tag="VI319_04670"
     CDS             186..2000
                     /locus_tag="VI319_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863171.1"
                     /translation="MSDAPRAAMSAAALARRLLAQARPYWAHLAGIVALDLLAVPLAL
                     LTPLPLKIAIDSALGGQGLPGPLARLGDAGIGALGMTTALAVAVGGIVAIALATELQK
                     LLTWLLQSWTGEKLVLAFRARLFAHVQRLSLAWHDTRGTADAIYRLEYDAPAIQWIGV
                     YGVSPFLTAALTLGGMIIVTARLDGSLALVALAVVPVLALLIRVFGGRLHAKWHEAKE
                     LGSSATSVLQEVLTALRVVKAFGQERREERRFVHQASQEVRANVELVRLQGAFYSLIG
                     LTLAAGTAAALWLGVSHVQQGRLSLGELVIVMAYLAQLYTPLDALSKNLSSLQRSVVG
                     AERAFALLDVLPEVADRPDARPLSRARGEIEFRAAGFAYERARPVLRDLSLRVPAGTR
                     VGIVGRTGAGKTTLVNLITRFYDPTEGAVLLDGEDLRRWRLEDLRRQFAIVLQEPVLF
                     STSIGENIAYGRARASEAQIEAAARAAGAHDFIAALPEGYRTPVGERGMRLSGGERQR
                     IALARAFLKDAPILILDEPTSSVDLKTEQGIIQALERLMHGRTTFIIAHRLSTLAHCD
                     VVYAVEDGRLAAATVDQSGPLLANRGLATDAPIRASGR"
     gene            complement(1987..2571)
                     /locus_tag="VI319_04675"
     CDS             complement(1987..2571)
                     /locus_tag="VI319_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6863172.1"
                     /translation="MNRLRAVAASVALLALAPGAALALDPEKLEGWWAGADVGYASMT
                     RHFSAQDESTTQHKMITGLRLGYSWDPRLLLGVEASNWLLKGANTWHLNEGQGIQTFN
                     VIAQAYPAGNRWWVKAGLGGARFWTDVPEEGTGRGFGGVLGFGYDVPLWGSFRLTPAI
                     DYAWGRIKDATSPAGVKQDQDFRAFAFKIGLTFR"
     gene            2666..3589
                     /locus_tag="VI319_04680"
     CDS             2666..3589
                     /locus_tag="VI319_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY6863173.1"
                     /translation="MKIRFLPLLAWALAAAPAFADTALPLSAPVPGGVAVVCLGRASE
                     PAPRVEFDGRRVLVARVGDTWRAVVGLPLALAPGRHALEVEDGDGGRSVAFEVVPHKY
                     GEQHVRLRDQRMVTPPKALLARIAREQETLLSAFSTWSEPALADLQLDLPAPGRISGG
                     FGMARFFNDQPRAPHSGLDIAAAAGTPILAPAAGIVIDTGQYYFNGRTIVLDHGEGLI
                     TMYNHLDHIGVAKGARVERGQVIGTVGHTGRVTAAHLHWTVSLNNARVDPALFLAGEV
                     REQLLPAYVPASASAASSTGTAESAAPGCGS"
     gene            3586..4989
                     /gene="asnS"
                     /locus_tag="VI319_04685"
     CDS             3586..4989
                     /gene="asnS"
                     /locus_tag="VI319_04685"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEY6863174.1"
                     /translation="MNTVLSVRAILGGQAPADRPVTVRGWVRTRRDSKAGVSFVHVSD
                     GSGFQPLQVVAPATLVNYASEVQKLTAGCAVEAAGTIVPSPAKGQPFEMQAQSLRVVG
                     WVEDPDTYPIQPKPHTLEFLREVAHLRPRTNVIGAASRVRHALAMAIHRFFHEEGFVW
                     VNTPIITASDAEGAGELFRVSTLDLANLPRTSDGKVDFAKDFFGREAFLTVSGQLNVE
                     TYCMALSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFADLADDAALAERLLKYVLK
                     AVLDERPDDMAFFDERIEKGIVAKLQGIVDKPFVRMDYTEAVRVLEGSRQKFEFPVRW
                     GTDLQSEHERWLCEQHVKGPLILVNYPKAIKAFYMRLNDDGRTVAAMDVLAPGIGEII
                     GGSQREERLAVLDARIVEAGLDPETYGWYRDLRRYGTVPHAGFGLGFERTVAYVTGLA
                     NVRDVIPFPRTPGSARY"
     gene            complement(5005..5586)
                     /locus_tag="VI319_04690"
     CDS             complement(5005..5586)
                     /locus_tag="VI319_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY6863175.1"
                     /translation="MIKLVRAGLLAVAVLTAPHAGARSEAQPKLVQKPQPRIATDRLE
                     KSIHALINRERAAKGLKPLAWDAKLAAIARGHSADMGKRNYFAHATPEGANFDARDAK
                     ARYKCFVPVGDVVHGGAENIARDDLWARITTLGDRKYYDWHSENELARRTVDGWMKSP
                     GHRANILTPHWMHEGLGVTVTRDLKVYVTEDFC"
     gene            complement(5643..5969)
                     /locus_tag="VI319_04695"
     CDS             complement(5643..5969)
                     /locus_tag="VI319_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863176.1"
                     /translation="MPRSHHYHVYVIELSKDVLDEPKFRRANADYRPGKPCVYVGMTG
                     LDPDLRFDKHKAGIQSNRYVFRYGLRLLPDLYECYNPMPYDAARDMEVELAIGLREDG
                     YGVWQG"
     gene            6008..6874
                     /locus_tag="VI319_04700"
     CDS             6008..6874
                     /locus_tag="VI319_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6863177.1"
                     /translation="MAGALEGVLTLPDGRRLGWAEYGDRRGRPVLAFHGLPGSRRQRH
                     PDDAIALGLGARTLHLERPGFGISDPAPGRTFAGWARDVAAACDLLGVERIRIAGVSG
                     GGPYALACAALLGPRVERVALISGVGPAEAMRARGHSLFVRLGFALAPHGAWTMRPFT
                     AAASAVGRRLPERYFELLGLGLNAADRRVLQRAEIRAMFVEDMAEAFAQSGAAMAEDL
                     ALVASRWDFDPGAIRAPVRSWHGSDDTIVPLRAAEAAAARVPGAELTVIAGEGHFLVF
                     ERWREILEWLTR"
     gene            6975..7157
                     /locus_tag="VI319_04705"
     CDS             6975..7157
                     /locus_tag="VI319_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY6863178.1"
                     /translation="MNKHQIAGAAKEIAGKAQETIGKLLGSGKEQIKGLKKQLTGKTQ
                     RAASRTRKAGRRVRAR"
     gene            complement(7174..8022)
                     /locus_tag="VI319_04710"
     CDS             complement(7174..8022)
                     /locus_tag="VI319_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY6863179.1"
                     /translation="MDATRVSGSTNALYSPAVALQFFKSEGKLQDIPQGKTIFAESEK
                     GLPFLLMRQRMYLLLEGEVSVLAKDTPIATLGKGEIFGEMASINQAPRSAAAVAKTPC
                     RVIALDDRQFQAALARRPEFALMLMSVMVHRLRENIGRLSAHDALKGVPWKEAAVLDE
                     QILAELGQQIGPAARFRYNAGKLIMKEGAVGVVSYVVLDGRVAITIGNAVMEKVGPGG
                     IFGEMALVERAPRLASAIAETDCTLLAISRLEFVRLVKNNPRFGAALLSAVSERARWT
                     ASRQAA"
     gene            8375..9193
                     /locus_tag="VI319_04715"
     CDS             8375..9193
                     /locus_tag="VI319_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY6863180.1"
                     /translation="MKRPFWLSAALALGLLGSLPARAEITLAVNEGVTYFVTAQEIRE
                     KYHELAEAIGKTLKTTVKVVPVDQYPVLRKGLDEQMYDLAFVHPAHHSLISMRDGKYR
                     LVVLTKGYTEYRARFFTAKGTALKQPSDMKGKRIGMPDPDSITAVITRATLRDLGIDA
                     SKAELRTTRYQDAVPFFVENGFSDVGVAASGAVVKSWQEKGGSVLFESKPVPIKHMIA
                     STKMNPGDVAKVRELMLGLDKSDAGRKILAKIGYQGYEAGDDQQLAALTKWLGY"
     gene            9210..10121
                     /locus_tag="VI319_04720"
     CDS             9210..10121
                     /locus_tag="VI319_04720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6863181.1"
                     /translation="MKTAAPAAVFFGSRYDRYKARAEAGMRIGGRAGRWAALACGALA
                     FAAAAQQPGASGYEPIPGQPGKDVVWVPMPDSQVERMLDLARVTAADFVVDLGSGDGR
                     TVIAAAKRGARAHGVEFNPDLVALSRERAREAGVADKATFAEGDLYKADLSRATVVTL
                     FLLDDINVRLRPALLALKPGTRVATNTFKMGDWEPDAETGAPGCYSWCFIYLWIVPAR
                     IEGTWRTPQGELSLTQQYQAVSGTLGSGPLGQAVHGRLSGDRIVLTAAGAEYRARLVG
                     DSMEGTVSTGGAIRPWQAGRGAVAPPR"
     gene            10261..10623
                     /locus_tag="VI319_04725"
     CDS             10261..10623
                     /locus_tag="VI319_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863182.1"
                     /translation="MTQLAYSIFTAHQRMPENDIALQIIELFERFPGLTAFTVGKDEA
                     AVAEQPAEPTVAIRNVSFGDARKEDEGEIMARIRDALVQFVHGRPEMLDLLSGRTFSR
                     TLHQLLDTRALQAQQRAA"
     gene            complement(10684..11010)
                     /locus_tag="VI319_04730"
     CDS             complement(10684..11010)
                     /locus_tag="VI319_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEY6863183.1"
                     /translation="MPMLRFGRILTLAAAVALSPGAIAAGKSHQVVIEGIKFVPERIE
                     VAVGDSITWVNKDPVPHTVTNAAKKIESGTIDPNGKWKYVVRRKGDIDYVCRFHPGMR
                     GTISVK"
     gene            complement(11010..11528)
                     /locus_tag="VI319_04735"
     CDS             complement(11010..11528)
                     /locus_tag="VI319_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY6863184.1"
                     /translation="MKNASITVLAIAAFAAGAANAQAPSDPQIAGIVVTANQIDIDAG
                     KLAKSHTKNKEVQGFANQMIKDHTAVNKQAVALAKKLGVKPAASDTQASLKKGAAENM
                     SNLKKLKGKQFDKAYIDHEVDYHQAVLDAIDKTLIPSAQNAELKGLIEKVRPAIAAHL
                     DHAKMIQSKLGG"
     gene            11666..12364
                     /gene="queC"
                     /locus_tag="VI319_04740"
     CDS             11666..12364
                     /gene="queC"
                     /locus_tag="VI319_04740"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY6863185.1"
                     /translation="MHETAMVLLSGGQDSTTCLAWALEHFGNVETVGFRYGQRHAVEL
                     DCRPVILAELRTRFPGWARKLGDDHVLDLGVIPALGATAMTHDIAIETGRSGLPNTFV
                     PGRNLLFFTAAAALAYRRGVRTLVGGMCETDYSGYPDCRDDTLKALQVALGLGMERRF
                     VIETPLMWIDKAQTWSLARALGGEPLLELIVEHTHSCYLGERSVRHPWGYGCGGCPAC
                     ELRRGGWERYRASS"
     gene            complement(12346..13689)
                     /locus_tag="VI319_04745"
     CDS             complement(12346..13689)
                     /locus_tag="VI319_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6863186.1"
                     /translation="MSTQFQLMRERRFRPLFFTQFLGAFNDNVFKTALVTLVTFRTGQ
                     LTDADPRMLVTLLPGLFILPFFLFSATSGQLADKLDKSRIARAVKIFEIAVMVLAAWG
                     FLAGSLWQLVTALVLMGVHSTVFGPVKYGYLPQHLRTEELIGGNGLIEMGTFVAILLG
                     EVLGAWLAAIPQGAVATSTTVVGVAVLGWWASRGIPYTPPVDPDLRVGPNFAAETWRN
                     LRFAHGNRVVWLSLIGISWFWFYGATLLAQFPVYAKDVLGGDESVFILLLTIFSVGIG
                     AGSLACERLSGGRVEIGLVPFGTIGLTLFGVDLYFASGSQAAAAGAHFTHFLREPSHW
                     RLLADLALLGVFGGFYIVPLYALIQTRSEKTHQSRVIAANNILNAAFMVASALASMAL
                     FAAGLSIPQLFLATALANAAVAAWIYARVPEFMQRFVAWVSRRPAAGSGRYDDAR"
     gene            complement(13686..14666)
                     /locus_tag="VI319_04750"
     CDS             complement(13686..14666)
                     /locus_tag="VI319_04750"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863187.1"
                     /translation="MTRALALVLASRTLCAAAILLIAASASAQYPTRPLRLLVPNPPG
                     GATDTIARVIAPGLGEALGQPVVVENRPGSNGNLSSELAAKAAPDGYTLLLGQDSQIV
                     ISPHLYRNMPVDTLKDLAPVASLVTTQMVLSVNPSLPVHTLPEFIEYARRASPPLAYA
                     SIGNGSQHHLTMEMLKARAGINLVHVPFKGGGPATLAVIAGEVPVMFGGNSVTGQIKA
                     GKLRGLAVAGKHRSETFPDLPTLSEFYPGLEVTAWLGLFTGGEVPAAIVTRLHAETNR
                     LLGDPATRAKIAKVGGLTPFATTTEEFTALIRSEYAKYGEVVKAVGATID"
     gene            14782..15288
                     /locus_tag="VI319_04755"
     CDS             14782..15288
                     /locus_tag="VI319_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEY6863188.1"
                     /translation="MKISMYQASAPRFVNMLTNLSAILDKAQAHAEARKIDPATLAND
                     RLFPDMFPMKRQVQIACDTAKGAVARLAGVDVPKHEDTEQTFPELKARIAKTIDFVKS
                     IPPAQLEGTEDKNIHLKLGPREVDFTGMQYLLGHALPNFYFHVTTAYDILRHNGVELA
                     KRDYIGTP"
     gene            15320..16012
                     /locus_tag="VI319_04760"
     CDS             15320..16012
                     /locus_tag="VI319_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863189.1"
                     /translation="MNRRAFLAAGGALVLGGCATPRLGEYEPTVVEAPQWRAGDSWTF
                     RRIDGYNGLPRDLLTRTVSATSPAGTRFVTVDQNGATRDDALFEAPGVQVSGLLSEDG
                     PIAGLFEPRLVVYDFPLHSGKEWRQGLYRIYGSGFRSYLSHVSRAEGWEDIAVGARTY
                     RAILIRRTFNFGARDSFTPRPLVHDETEWYVPELRGTASLQTNEWLQAPPGGFSWPGY
                     RYVYTLDSFRLV"
     gene            16119..16316
                     /locus_tag="VI319_04765"
     CDS             16119..16316
                     /locus_tag="VI319_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY6863190.1"
                     /translation="MNKDQVKGSAKRLAGKAQEKTGKLTKNRKQQAKGLAKQAAGKVQ
                     KAYGDARDDLDKDREDSGRDG"
     gene            16440..17258
                     /locus_tag="VI319_04770"
     CDS             16440..17258
                     /locus_tag="VI319_04770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6863191.1"
                     /translation="MTAWRFPRRWAVALLVLAVPAGALAQTAASSQFQPEVGQQGKDV
                     VWVPTPEALVQKMLDMAKVTPKDFVMDLGSGDGRTVIGAAKRGAHALGIEYNPDMVEL
                     SRRNAKQAGVTDRAKFERADLFKTDLSRATVITMFLLTDINLRLRPTILNLKPGTRVV
                     SNTFRMGDWDPDETAELGCSSYCTAYLWIVPAHVEGSWKTAQGELTLRQTYQTVSGTL
                     SSGGKSLPVQRGKLRGDQISFTAGGAEYRGRVGDKMIEGTVKSGAKSERWSANR"
     gene            17267..18247
                     /locus_tag="VI319_04775"
     CDS             17267..18247
                     /locus_tag="VI319_04775"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6863192.1"
                     /translation="MRPLLFAAAIAAALATGAAGAADADAVRVNAFPNAKSLPLHAGV
                     EKGIFAARGLAVRLSFTENSTKQRQGLAAGEFDVVHAAVDNAVAMADGGRDVVIVTGG
                     DSGMNQFFVQPGIGSFADLRGRVLVVDAPNTAYALLAKKMLQKHGLLEGRDYTVKPVG
                     RGELRLKAMAADPANAAAILNLPYTIQAEAMGMKSLGNPVDALGPYQANGAFALRAWA
                     ERNRGLLERYIAAYVESLRWVLDPRHRDECIAMLQRELKITPEVAARAYRQLADPAAG
                     FTPDARFDMQGFRNMLALRQEIEGGGRPAPAPERYVDLSYYERALATLSR"
     gene            complement(18214..19095)
                     /locus_tag="VI319_04780"
     CDS             complement(18214..19095)
                     /locus_tag="VI319_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6863193.1"
                     /translation="MPFATINGIRIHYAVQGAGPHLLMFAPGGWRSVMSRWTAAGGKE
                     AFGEMDGLATLSRHFTTIAYDRRECGLSGGRVEPLAWDLYVAEAKALLDLAGAKRAWV
                     LGSCMGASLALAFAARHPQACLGLLLHWPVGGYLWMKKGHGFFQRHIDFVRANGLAAV
                     VARAPKGENFWYDPEIGPWGSPAAVYLEFAAELARQDVGRYLEICAQSRDAIFNDTMP
                     SGASGDELMRMDMPALILSGADTRHTVSSAWALKELMPRAELWDVLPPRQTGANVLEQ
                     VLRFASPQRDKVASARS"
     gene            19138..19602
                     /locus_tag="VI319_04785"
     CDS             19138..19602
                     /locus_tag="VI319_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863194.1"
                     /translation="MESKWKHQGVRVVRSGELDVNTAQTPGMNRAAAITYARAGAEKL
                     WAGTVVIHPKAKTGAHHHGPVESVIYVVSGRARMRWGERLEFVAEAGPGDFIYVPPYV
                     PHQEINASDEQPLSCVLVRSGQQPVVVNLDIPAVEQPEQVHWVDNIHPAPKA"
     gene            19978..20307
                     /locus_tag="VI319_04790"
     CDS             19978..20307
                     /locus_tag="VI319_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863195.1"
                     /translation="MKTLQIEQLSPQLRRVEEGFAALVHVLFRTYPELLCFSLGGRAD
                     LPPGMDIGDTEAQIFITDVDFGKPISQEYQNEIGEALGGIVSDIVTERPEALEVLPGR
                     TFARTLQ"
     gene            complement(20314..21177)
                     /locus_tag="VI319_04795"
     CDS             complement(20314..21177)
                     /locus_tag="VI319_04795"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY6863196.1"
                     /translation="MSFSERRQRLRALLSGTRCIYPGSVYDALSARIAEDLGFEAGMF
                     AGSIASLAVLGAPDLVVLTLTEFAEQARRICRAGTLPLIVDADHGYGNALNVRRTVEE
                     LENAGVAALTIEDTLLPAPFGGGKDQLIPTAEATGKMRAALSARGDPALVVVGRTSAL
                     PFAGVEEAVARAKAYEAAGVDALFFVGMKSRAQLDAIAPSVGVPIFLGNVGPELMDRD
                     YLGSRGVRVCLQGHQPFMAAVRAVHETLKALREGTPPAQITGVAAADLVKRATRDSDY
                     RRWMREFLGSP"
     gene            21324..22265
                     /locus_tag="VI319_04800"
     CDS             21324..22265
                     /locus_tag="VI319_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HEY6863197.1"
                     /translation="MLPTSKTNRPADLFAGKPSSFQAVLAEVEEFWTPLPDKPEETAE
                     AVARALWLTAAGTPMSAERCVALALPALDEQALGRLRTLLERKRDGEPLAHLTGRQTF
                     MGLDLLAGPSALIPRKETEILGRALAGKLDQIARERDEIRVIDLCTGCGNLALACASH
                     EPRARVWGADLSPEAVALARLNAERCGLASRVEFREGDLFAPFQDAEFLGRCDLVSCN
                     PPYISSSKVPGMHREISGFEPGLAFDGGTFGISVLTRFLREAPRFLKPGAWLALEIGA
                     GQGPATARQLAKNPALAEVETRSDAGGEIRAVLARAR"
     gene            22342..23328
                     /locus_tag="VI319_04805"
     CDS             22342..23328
                     /locus_tag="VI319_04805"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6863198.1"
                     /translation="MRTIAIEEHYTTAMYRQQVPANEFRNFYLTSRSEQLGHDIVDEL
                     GDLGARRIAAMDAAGIDVQVLSFGSPGPQGFAKEMAIPMARDANDRVHEAMQAHPARF
                     EGFAALPTADPAAAADELERCVRKLGFRGAMIHGHQQGEFLDDRKYWPVFERAEALGV
                     PVYLHPTLAHPKALESYFKGYEELARAAWGFAIDTSCHFLRIVFAGVFDAYPRLRIIL
                     GHLGEGLPFAMHRLDDHAGRAAARRGLKKRPIEYLRDNLLVTTSGNWYEPAFVCTLLA
                     LGADNILFAADWPYEANLTAAAFLGKISISEGDREKIAFRNAERLLGIRGAS"
     gene            23325..25055
                     /locus_tag="VI319_04810"
     CDS             23325..25055
                     /locus_tag="VI319_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY6863199.1"
                     /translation="MSLSEVREGMRIEWDVPITMDDGLVLRADVFRPEAEGRYPVILS
                     YGPYGKGLAFQDGYKTAWEIMARDYPDAVAGSSNRHANWEVVDPEKWVPDGYACVRVD
                     SRGAGRSPGFLCHNNARETRDIYLCIEWAAQQAWCSGKVGMNGISYYASNQWRAAAAN
                     PPHLAAICVWEGWNDAYRESARHGGIICSFRKNWQDMQVKTVQHGQGTRGARSRVTGE
                     LVCGPETLPPDELVKNREDMWQAFLSREMWDRYYAERSPDLARVNVPLLSAANWGGQG
                     LHSRGNFEGFMFAGTKQKWLEVHGGSHWAPFYTDYGVKLQKRFFAHFLKEEKNGWERQ
                     PRVLLNVRHPGEKYVLREENEWPLARTKWTRFHLDPKGMRLGTGEVGAVSSLAYDALG
                     DGLTFSTPPLAKPMEITGPSSLKLSLSSSTADADVFAVLRVFDPKGREVLFQGALDPK
                     TPVAQGWLRASHRKLDPRRSLPYRPFHTHDQKQPLEPGKVYELEVEIWPTCIVVPAGY
                     RIALSIRGRDYEHDEAPATLSNMKNPMRGCGPFTHDDETDRPPKVFGGKVTLHFGAGR
                     PASLLLPVIP"
     gene            complement(24997..25458)
                     /locus_tag="VI319_04815"
     CDS             complement(24997..25458)
                     /locus_tag="VI319_04815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6863200.1"
                     /translation="MDVLHPKGWAAPIGYANGIAASGRIVFVAGQVGWDERQQFQSED
                     IAPQFEQALRNVLAVLAEAGARPEHVCRMTAYCCDKPRYLRARKELGAIWRRHMGRHY
                     PAMSMVFVADLLDSPGKIELEATAVVPVRAGSARGSRAAAAKRGGRRRSAA"
     gene            complement(25467..26042)
                     /locus_tag="VI319_04820"
     CDS             complement(25467..26042)
                     /locus_tag="VI319_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEY6863201.1"
                     /translation="MSARPAPKATRKATRRKAAGRARASDQADFLARAWTMEVEAAER
                     YLTFADAMQTHNNPEVAELFRKLARIEQMHADQILEHSLATHPPVLPPGGVRWGAGRE
                     GPETADPGDLHYRMQPYHALQVALACERRARAYFAEVARSAPLPAVRKAAREMAEEEA
                     EHIELIEEWISRTPAPDKDWQHDPDPPVFSD"
     gene            complement(26039..26872)
                     /locus_tag="VI319_04825"
     CDS             complement(26039..26872)
                     /locus_tag="VI319_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY6863202.1"
                     /translation="MSSVPSAAPTAQAFKSDYKPIWCPGCGDYSVLSSITKAFAMLAL
                     RPENVAVVSGIGCSSRIPAYTNCYGFHGVHGRALAAGTGLKIARPDLTVMVASGDGDG
                     YSIGGNHFLHACRRNVDITYIVMDNHVYGMTKGQPSPTTEPDWDSKLSPGGTGLRSFH
                     PLVIALAAGANFVARAFAGDPNGTAEIIADAIRTPGFSFVEILSPCVTFRPEQREWKD
                     KVRAAPVAVTDDAARAARRIMTDDGFNIGVLYKGNRKPFARSAGAAVKRPADLESDFA
                     L"
     gene            complement(26869..28611)
                     /locus_tag="VI319_04830"
     CDS             complement(26869..28611)
                     /locus_tag="VI319_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY6863203.1"
                     /translation="MTDRSDAVAAASSVSVALTGSGGSGVMTAGNVLLEAAARAGWYG
                     LMVRSSGPQIRGGEAAALLRFAARPIDCLDDRFDALLAIDWQNVHRFADEIPIDGASL
                     MVGDPDQGEPPEVFVKRAPRRASVAFKKTAKSIPGSWPNMVMVGVAGALVGLPADAVK
                     AVLRQSQKRGQDALAATLAAVDAGYAAATQLPGVPRLAPPRRAAAPRWLISGNEAAGY
                     GALRGGVRFVAAYPITPATEVLEWMAPALAEVGGALVQAEDELASINMAIGASFGGVP
                     SLTATSGPGLALMTEALGLAVAAEVPIVVVDVMRGGPSTGIPAKSEQSDVNIAVKGLH
                     GDAPRVVVAPNSVADCLTATQWAVHLAEAMQAPAIVLSDQYLGQTRATVDRPADMSFI
                     GERAVSAARADGYKRYALTESGISPMAVPGTPGCAYTADGLEHNERGIPSSQSADHAA
                     QLAKRERKLKQHDYGALWAEIEGDGDTAVITFGSCTGPVREALARAAQDGLKARLVSM
                     RLLSPAQPERLAQALAGVKRVLVVEQNHGAQFHRYLRSDFDLPGEVRSLHRPGPLPIA
                     PGEVYRELVEWSRA"
     gene            28733..>29437
                     /locus_tag="VI319_04835"
     CDS             28733..>29437
                     /locus_tag="VI319_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY6863204.1"
                     /translation="MNAPAAPFVLKSHDLVDGWTARGVRVEKRAARTPDGRTVPGLYN
                     AWIWLDNEAQYNSYTTEMAKGIVLAFRAASNSRDVNCVVFTGVGDKAFCTGGNTKEYA
                     EYYAGRPQEYRQYMRLFNDMVSSILACDKPVICRVNGMRVGGGQELGMACDFSIAQDL
                     ARFGQAGTKHGSAPIGGATDFLPPVMGADRAMVACVLCEPFSAHKAYHWGVLADIAPA
                     LKVDGKYVANPLVETQR"
BASE COUNT         4478 a        10200 c        10308 g         4451 t
ORIGIN      
        1 cgccgtcgcg acggtcgccg cgctcggcgc gctcgccgtc ctcgcgctcc tcgaccgcgg
       61 ctgggtcgcg gctgcggcgc tcgccgcgct ggacgggctg ctcgcctggc gcattgcgcg
      121 cgactgcggc gccgcgctcg cgtcgctgga gcgcgcgctc gccgaggccg agcgatgagg
      181 ccgccgtgag cgacgccccg cgcgccgcga tgagcgccgc cgcgctcgcc cggcggctgc
      241 tcgcgcaggc gcggccgtac tgggcccatc tcgccggcat cgtcgcgctc gacctgctcg
      301 cggtgccgct cgcgctgctc acgccgctgc ccctgaagat cgcgatcgac agcgcgctcg
      361 gcgggcaggg gctgcccggg ccgctcgccc gcctcggcga cgccgggatc ggggctctcg
      421 ggatgacgac ggctctcgcc gtggccgtcg gcggcatcgt cgcgatcgcg ctcgcgaccg
      481 agctgcagaa gctcctcacc tggctgctgc agtcgtggac cggcgagaag ctcgtgctcg
      541 cgttccgcgc caggctgttc gcgcacgtgc agcgcctgtc gctcgcgtgg cacgacacgc
      601 gcggcacggc cgacgcgatc taccgcctcg agtacgacgc gcccgcgatc cagtggatcg
      661 gcgtgtacgg ggtgtcgccc ttcctcaccg ccgcgctcac cctcggcggc atgatcatcg
      721 tcacggcgcg cctcgacggc tcgctcgcgc tggtcgcgct cgcggtcgtg ccggtgctcg
      781 ccttgctcat ccgcgtcttc ggcgggcggc tgcacgccaa gtggcacgag gcgaaggagc
      841 tgggcagctc ggccacctcg gtgctgcagg aggtgctcac cgcgctgcgc gtggtcaagg
      901 cgttcgggca ggagcgccgc gaggagcgcc gcttcgtcca ccaggcgtcg caggaggtgc
      961 gcgccaacgt cgagctggtg cgcctgcagg gagcgttcta ctcgctcatc gggctgacgc
     1021 tcgccgcggg caccgcggcg gcgctgtggc tgggcgtgtc gcacgtgcag cagggtcgcc
     1081 tttcgctggg cgagctcgtg atcgtgatgg cctatctcgc gcagctctac acgccgctcg
     1141 acgcgctgtc gaagaacctg tcgagcctgc agcgctcggt ggtgggcgcc gagcgcgctt
     1201 tcgcgctgct cgacgtgctg cccgaggttg ccgaccgacc cgacgcgcgg ccgctgtccc
     1261 gcgcgcgcgg cgagatcgag ttccgcgccg cgggcttcgc ctacgagcgc gcgcgcccgg
     1321 tgctgcgcga tctgagcctg cgcgtgcccg ccggcacgcg cgtcggcatc gtcgggcgca
     1381 ccggcgccgg caagaccacg ctcgtcaacc tgatcacgcg cttctacgat cccaccgagg
     1441 gcgcggtgct gctcgacggc gaggacctgc ggcgctggcg gctcgaggac ctgcgccgcc
     1501 agttcgcgat cgtgctgcag gagcccgtgc tgttctcgac ctcgatcggc gagaacatcg
     1561 cctacggccg cgcgcgcgcg agcgaagcgc agatcgaggc ggcggcgcgc gccgcgggcg
     1621 cgcacgactt catcgccgcg ctgcccgagg gatatcgcac cccggtcggc gagcgcggca
     1681 tgcgtctctc gggcggcgag cgccagcgca tcgcgctcgc gcgcgccttc ctgaaggacg
     1741 cacccatcct catcctcgac gagccgacca gctcggtcga cctgaagacc gagcagggca
     1801 tcatccaggc gctggagcgg ctgatgcacg ggcgcacgac gttcatcatc gcgcaccggc
     1861 tgtcgacgct cgcgcactgc gacgtcgtct acgcggtgga ggacgggcgg ctggccgccg
     1921 cgacggtcga ccagtcggga ccgctgctcg cgaaccgcgg gctcgcgacg gacgcgccga
     1981 tccgggctag cggaaggtga ggccgatctt gaaggcgaag gcgcggaagt cctgatcctg
     2041 cttcacgccg gcgggcgagg tcgcgtcctt gatgcgcccc caggcgtagt cgatcgcggg
     2101 cgtcagccgg aacgagcccc acagcggcac gtcgtagccg aacccgagga cgccgccgaa
     2161 gccgcggccg gtgccttcct cgggcacgtc ggtccagaat cgcgcgccgc cgagaccggc
     2221 cttgacccac caccggttgc ccgccgggta ggcctgcgcg atgacattga aggtctggat
     2281 gccttggccc tcgttgaggt gccacgtgtt ggcgcctttc agcagccagt tcgacgcctc
     2341 gacgccgaga agcaggcgcg gatcccagct gtagccgaga cgcagcccgg tgatcatctt
     2401 gtgctgcgtc gtcgactcgt cctgggccga gaagtgccgc gtcatcgagg cgtagccgac
     2461 gtcggcgccg gcccaccagc cttcgagctt ctcgggatcc agcgcgagcg ccgcgcccgg
     2521 cgcgagagcg agcagggcga cggaagcggc gacggcgcgc aggcggttca tcgggcgact
     2581 cacgggctca gacagcggtt cggcgttttc gtgctcgccg gcgatggtat cctctttccg
     2641 tgtgtcaggc attttccgac cttccgtgaa aattcgattt ctcccgctcc tcgcgtgggc
     2701 gctcgccgcg gcgcccgctt tcgccgacac ggcgctgccg ctttcggctc ccgtgcccgg
     2761 cggcgtcgcc gtcgtctgtc tcgggcgcgc ctcggagccc gcgccccgcg tcgagttcga
     2821 cggccgccgc gtgctggtcg cgcgcgtcgg cgacacgtgg cgagccgtgg tggggctgcc
     2881 gctcgcgctc gccccgggcc ggcacgcgct cgaggtcgag gacggcgacg gcggccgcag
     2941 cgtggcgttc gaggtcgtgc cgcacaagta cggcgagcag cacgtgcggc tgcgcgacca
     3001 gcgcatggtg acgccgccga aggcgctgct cgcgcgcatc gcgcgggagc aggagaccct
     3061 gctgagcgcg ttctcgacct ggagcgagcc ggcgctcgcc gacctgcagc tcgacctgcc
     3121 cgcgccgggg cgcatctcgg gcgggttcgg gatggcgcgc ttcttcaacg atcagccgcg
     3181 cgcgccgcac agcggcctcg acatagcggc ggcggccggg acgccgatcc ttgcgccggc
     3241 cgccggcatc gtcatcgaca ccggccagta ctatttcaac ggccgcacca tcgtcctcga
     3301 ccacggcgag ggcctgatca cgatgtacaa ccacctcgac cacatcggcg tggcgaaagg
     3361 cgcccgcgtc gagcgcggcc aggtcatcgg caccgtggga cacaccggcc gcgtcaccgc
     3421 tgcgcacctg cactggaccg tgagcctcaa caacgcgcgc gtcgatccgg cgctgttcct
     3481 cgcgggcgag gtgcgcgagc agctcctgcc ggcgtacgtg cccgcgtcgg cttcggccgc
     3541 atcgtcgacc gggacggcgg aatccgcggc gccgggctgc ggatcgtgaa caccgtgctg
     3601 tcggtgcgcg ccatcctcgg cggccaggcc ccggccgacc gcccggtgac cgtgcgcggc
     3661 tgggtgcgca cccggcgcga ctcgaaagcg ggcgtctcgt tcgtgcacgt ctcggacggc
     3721 tcgggattcc agccgctgca ggtggtggcg ccggcgacgc tcgtgaacta cgcatccgag
     3781 gtgcagaagc tcaccgccgg ctgcgcggtc gaggcggccg gaacgatcgt gccgtcgccg
     3841 gcgaagggac agccgttcga gatgcaggcg cagtcgctgc gcgtcgtcgg ctgggtcgag
     3901 gaccccgaca cctatccgat ccagcccaag ccgcacacgc tcgagttcct gcgcgaggtg
     3961 gcgcacctgc ggccgcgcac caacgtgatc ggcgcggcgt cgcgcgtgcg gcacgcgctc
     4021 gcgatggcga tccaccgctt cttccacgag gaaggcttcg tctgggtcaa cacgccgatc
     4081 atcaccgcct ccgacgccga aggcgcgggc gagctcttcc gcgtgtcgac gctcgatctc
     4141 gcgaacctgc cgcgaacgag cgacggcaag gtggacttcg cgaaggactt cttcgggcgc
     4201 gaggccttcc tcaccgtgtc ggggcagctg aacgtcgaga cctactgcat ggcgctgtcg
     4261 aaggtctaca ccttcggccc gacgttccgc gccgagaact cgaacacgag ccggcacctc
     4321 gccgagttct ggatgatcga gcccgagatc gcgttcgccg acctcgcgga cgacgccgcg
     4381 ctcgccgagc ggctgctgaa atacgtgctc aaggcggtgc tcgacgagcg ccccgacgac
     4441 atggcgttct tcgacgagcg catcgagaag gggatcgtcg ccaagctcca gggcatcgtc
     4501 gacaagccgt tcgttcgcat ggactacacc gaggcggtgc gcgtgctcga gggctcgagg
     4561 cagaagttcg aattcccggt gcggtggggc accgacctgc agagcgagca cgagcgctgg
     4621 ctgtgcgagc agcacgtgaa gggcccgctg atcctcgtga actatccgaa ggcgatcaag
     4681 gcgttctaca tgcgcctgaa cgacgacggc cggacggtcg cggcgatgga cgtgctcgcg
     4741 ccggggatcg gcgagatcat cggcggctcg cagcgcgagg agcggctcgc ggtgctcgac
     4801 gcgcgcatcg tggaagccgg gctcgatccc gagacctacg gctggtaccg cgacctgcgc
     4861 cgctacggca ccgtgccgca cgcgggcttc ggcctgggct tcgagcgcac ggtcgcgtac
     4921 gtcaccggcc tcgccaacgt gcgcgacgtg attccgtttc cgcgcacgcc gggcagcgcg
     4981 cgatattgac gaacctgcgg agcctcagca gaaatcctcg gtcacgtaga ccttgaggtc
     5041 gcgcgtgacg gtcacgccca gcccctcgtg catccagtgc ggggtgagga tgttcgcgcg
     5101 gtgcccggga ctcttcatcc agccgtcgac cgtgcggcgc gcgagctcgt tctcggagtg
     5161 ccagtcgtag tatttcctgt cgccgagcgt cgtgatcctg gcccagaggt cgtcgcgcgc
     5221 gatgttctcg gccccgccgt ggacgacgtc gccgaccggc acgaagcact tgtagcgcgc
     5281 cttcgcgtcg cgcgcgtcga agttggcgcc ttcgggcgtg gcgtgggcga aatagttgcg
     5341 cttccccatg tcggcgctat gcccgcgggc gatcgcggcg agcttcgcgt cccaggcgag
     5401 gggtttgaga cctttcgcag cgcgctcgcg gttgatgagg gcgtggatcg acttctccag
     5461 gcgatcggtc gcgattcgcg gctgcggctt ctgcacgagc ttcggctgcg cctccgaccg
     5521 cgcgcccgcg tgcggcgcgg tcaaaacggc gacggcgagc agtcccgcgc ggacgagctt
     5581 gatcatggtt cctcccggga cgcatcgtag cccgggcggc gccgccgcgg cgatggaatt
     5641 cttcacccct gccagacgcc gtacccatcc tcgcgcagac cgatcgcgag ctcgacctcc
     5701 atgtcgcgcg cggcgtcgta gggcatgggg ttgtagcatt cgtagaggtc ggggagcagc
     5761 cgcaggccat agcggaatac gtagcggttc gactggatcc ccgccttgtg cttgtcgaag
     5821 cgcagatcgg ggtcgagacc cgtcatgccg acgtagacgc acggcttgcc cggacggtag
     5881 tcggcgttgg cgcggcggaa cttcggctcg tcgagcacgt ctttcgacag ctcgatcacg
     5941 tagacgtgat aatgatggct ccgcggcacg ccggctcccg cgcgacagaa taacaggagg
     6001 tgaaacgatg gcgggtgcgc tcgagggcgt gctgacgctg cccgatggca gacggctcgg
     6061 ctgggccgaa tacggcgatc gccgcggccg gccggtgctc gcgttccacg gactgccggg
     6121 ctcgcgccgg cagcgccatc cagacgacgc catcgcgctc ggcctcggcg cccgcacgct
     6181 gcatctcgag cggcccggct tcggcatctc cgatcccgcc cccgggcgca cgttcgcggg
     6241 atgggcgcgc gacgtcgccg cagcttgcga cctgctcggc gtcgagcgca tccgcatcgc
     6301 gggcgtgtcg ggcggcggcc cgtacgcgct cgcctgcgcc gccttgctcg gtcctcgcgt
     6361 cgagcgggtc gctctgatca gcggcgtcgg gccggccgag gcgatgcgcg cccgcggcca
     6421 ttcgctgttc gtgcgcctcg ggttcgcgct cgcgccccac ggcgcgtgga cgatgcgccc
     6481 gttcaccgcg gccgcgagcg cggtcggccg gcggctgccc gagcgctatt tcgagctgct
     6541 cgggctcggg ctgaacgcgg ccgaccggcg cgtgctccag cgcgcggaga tccgcgccat
     6601 gttcgtcgag gacatggccg aggccttcgc gcagtcgggc gccgcgatgg cggaggacct
     6661 tgccctcgtc gcctcgcgct gggatttcga tccgggcgcg atccgcgcgc cggtgcgctc
     6721 ctggcacggc agcgacgaca cgatcgttcc gctgcgcgcc gccgaggccg ccgcggcgcg
     6781 cgtgccgggc gccgagctga ccgtgattgc gggcgaaggc cacttcctgg tgttcgagcg
     6841 ctggcgcgag atcctcgagt ggctgacgcg ctagcggcga ccgcgccttt cggcggacgc
     6901 tgtgcgccct cgccgtcgcg aattcgcaca agaatcgcgt gacaccattg cccgggtcac
     6961 caaaggagat ccacgtgaac aagcatcaga tcgcgggcgc ggcgaaagag atcgcaggca
     7021 aggcgcagga aaccatcggc aagctgttgg gcagcggcaa ggagcagatc aagggcctga
     7081 agaagcagct gaccggcaag actcagcgcg ccgcctcgcg cacgcgcaag gccggccgca
     7141 gggtgcgcgc gcgctagaac gaagctgcgg ccctcaggcg gcctggcgcg acgcggtcca
     7201 gcgggcgcgc tcgctcaccg ccgagagcag cgcggcgccg aagcgcggat tgttcttgac
     7261 cagccggacg aactcgagcc ggctgatcgc gagcagcgtg cagtcggtct cggcgatcgc
     7321 gctcgcgagc cgcggcgcgc gctcgaccag cgccatctcg ccgaagatcc cgccggggcc
     7381 gaccttctcc atgacggcgt tgccgatcgt gatcgcgact cgcccgtcga gaacgacgta
     7441 cgagacgacg ccgaccgcgc cttccttcat gatgagcttg ccggcgttgt agcggaagcg
     7501 ggcggccggc ccgatctgct ggccgagctc ggccagtatc tgctcgtcga gcacggcggc
     7561 ttccttccag gggacgccct tcagcgcgtc gtgcgcgctc aggcgtccga tgttctcgcg
     7621 cagccggtgg accatgacgc tcatcagcat cagggcgaac tccgggcgcc tggcgagcgc
     7681 ggcctggaac tggcggtcgt cgagcgcgat gacgcggcac ggggtcttcg cgaccgccgc
     7741 ggcgctgcgc ggcgcctgat tgatcgaggc catctcgccg aagatctcgc ccttgcctag
     7801 ggtggcgatc ggcgtgtcct tggcgagcac gctgacctcg ccctcgagca gcaggtacat
     7861 gcgctggcgc atcagcagaa agggaaggcc cttctcgctc tcggcgaaga tggtcttacc
     7921 ctgcggaatg tcctggagct tgccttcgga tttgaagaac tgcagcgcca cggccgggct
     7981 gtagagcgcg ttcgtgctgc cggagacacg ggtcgcatcc atggggcctc cctttcgagc
     8041 cgcgccggcc tcgccttctt ggtgcgcctg gcgcgcgggg aggtgattat gcagtcggca
     8101 tacccggttg cccccggaaa cgagcgaaaa atcgtttcaa attccacagt tccgcccgac
     8161 ttcgcacccc cggcacgcct gacgaggccc cgacatggag gcggtagcca caagtccgcc
     8221 gtcgatttga ggtgaatcac gttctctgaa cagaagttca tttcaacaca atcacttatc
     8281 aatatcagtt cctggtgtcg gcaaccgggt ataactcttg catcagaaaa cgctaaagca
     8341 cgccaggacg tgcttcaaac aaaggagaac acagatgaaa cgtccgtttt ggctgtccgc
     8401 ggcgctggcg ctcgggctcc tcggctcgct tcccgcgcgc gctgaaatca cgctcgcggt
     8461 gaacgagggc gtgacctact tcgtcaccgc gcaggagatc cgcgagaagt accacgagct
     8521 ggccgaagcg atcggcaaga cgctgaagac gacggtgaaa gtcgtgcccg tcgaccagta
     8581 cccggtgctc cggaagggcc tcgacgagca aatgtacgac ctcgcgttcg tgcaccccgc
     8641 gcatcactcg ctgatctcga tgcgcgacgg caagtaccgc ctggttgtcc tgaccaaggg
     8701 ctataccgaa tacagggcgc gcttcttcac ggcgaagggc acggcgctca agcagccgtc
     8761 cgacatgaag ggcaagcgca tcggcatgcc ggaccccgac tcgatcaccg cggtgatcac
     8821 gcgggctacg ctgcgggacc tcgggatcga cgcctccaag gcggagctgc ggaccactcg
     8881 ctatcaggac gcggtcccgt tcttcgtcga gaacggattc agcgatgtcg gcgtggcggc
     8941 gtcgggcgcc gtcgtgaaat cgtggcagga gaagggcggc tcggtgctct tcgagtcgaa
     9001 gcccgtcccg atcaagcaca tgatcgcgtc gacgaagatg aatcccggcg acgtcgccaa
     9061 agtgcgcgag ctgatgctcg gcctggacaa gagcgacgcg ggccgcaaga tcctcgcgaa
     9121 gatcggctat cagggatacg aggccggaga cgaccagcag ctggcggcgc tcaccaagtg
     9181 gctgggctat tgacgagccg catggaccga tgaaaacggc ggctccggcc gccgtttttt
     9241 ttggaagccg ctatgatcgt tacaaggcgc gagcggaggc cgggatgagg atcggcggac
     9301 gggcggggcg gtgggcggcc ttggcgtgcg gtgcgctggc atttgcggcg gcggcgcagc
     9361 agccgggcgc atcgggctac gagcccattc cgggccagcc cgggaaggac gtggtgtggg
     9421 tgcccatgcc cgactcgcag gtggagcgaa tgctcgatct cgccagggtg acggctgccg
     9481 acttcgtcgt cgatctcggg tcgggcgacg gacggaccgt gatcgcggcc gcgaagcgcg
     9541 gcgctcgcgc gcacggggtg gagttcaatc ccgacctggt cgcgctctcg cgcgagcgcg
     9601 ctcgcgaggc tggcgtcgcg gacaaagcga cgttcgcgga gggcgacctg tacaaggcgg
     9661 acctgtcgcg ggcgaccgtg gtcaccctgt tcctgctcga cgacatcaat gtccggttgc
     9721 ggcccgcgct gcttgccctc aaaccgggga cccgcgtcgc gaccaatacc ttcaagatgg
     9781 gcgactggga gccggacgcc gagacgggcg ctcccggctg ctattcctgg tgcttcatct
     9841 acttgtggat cgtcccggcg cgcattgaag gcacttggcg taccccgcag ggcgagctct
     9901 cactcacgca gcaataccag gcggtttcag ggacgctcgg ctcggggccc ctggggcagg
     9961 cggtgcacgg gcggctgtcc ggggaccgga tcgtattaac ggcggccgga gcggagtacc
    10021 gggcccgcct cgtcggcgac tcgatggagg gtacggtcag caccgggggc gcgatccgtc
    10081 cctggcaggc gggccgcggg gccgtggcgc cgccccgctg aagtactccc ttaggactat
    10141 ccggcaactg ccggtccggc ctcaggtttt tcgaaggcta cgccgggatt cacccgattc
    10201 atcgggccac cctcgaccga tacgatgccc tcactgggac ttgtcgaggc gaggatttcc
    10261 atgactcagc tggcttattc gatcttcacc gcgcatcagc gcatgcccga gaacgatatc
    10321 gcgctgcaga tcatcgagct gttcgagcgc tttccgggcc tgacggcctt caccgtcggc
    10381 aaggacgagg ccgccgtggc cgagcagccc gccgagccta cggtcgcgat ccgcaacgtc
    10441 agcttcggcg acgcccgcaa ggaagacgag ggcgagatca tggctcgcat ccgcgacgcg
    10501 ctcgtgcagt tcgtgcatgg gcgtcccgag atgctggatc tgctcagcgg ccggacgttc
    10561 agccgcaccc tgcaccagct gctggacacg cgcgccctgc aggcgcagca gcgcgccgcc
    10621 taaggcacct acaacacgct cggggagaga cgcgcgggtc cggtcaaacc ggcccgcgcg
    10681 ttttcatttc acgctgatcg ttccgcgcat gccggggtgg aaccggcaca cgtagtcgat
    10741 gtcgcccttg cggcgcacga cgtatttcca cttgccgttc gggtcgatcg ttcccgactc
    10801 gatcttcttc gcggcgttgg tcacggtgtg aggcaccggg tccttgttga cccaggtgat
    10861 ggagtcgccg accgcgacct cgatgcgctc ggggacgaat ttgatcccct cgatgacgac
    10921 ctgatgcgac ttgccggcgg cgatcgcgcc cggtgagagc gcgaccgccg cagccagcgt
    10981 gaggatccgg ccgaaccgga gcatcggcat cagccgccga gcttgctctg gatcatcttg
    11041 gcgtgatcca ggtgcgcggc gatggccggg cgcaccttct cgatcagccc cttcagctcg
    11101 gcgttctgcg cgctcgggat gagggtcttg tcgatcgcgt cgagcacggc ctggtggtaa
    11161 tcgacctcgt ggtcgatgta ggccttgtcg aattgcttgc ccttcagctt cttcaggttg
    11221 gacatgttct ccgcggcgcc tttcttcagg ctcgcctgcg tgtcgctcgc cgccggcttg
    11281 acgccgagct tcttcgcgag cgcgaccgcc tgcttgttca ccgcggtgtg gtccttgatc
    11341 atctggttcg cgaagccctg cacttccttg ttcttcgtgt gcgacttcgc gagcttgccc
    11401 gcgtcgatgt cgatctggtt cgcggtgacg acgatgcccg cgatctgcgg gtcgctcggc
    11461 gcctgggcat tggccgcgcc ggcggcgaac gccgcgatcg cgagcacggt aatggatgcg
    11521 tttttcatgt ctaccttctc ctttccatga gtagggtttt ttcgacgggt tgacgtccgc
    11581 cataggatgc tcgaccgggg agccgcgttc ccgcagccgc cggtatgatg agcgcgtgat
    11641 ccagccggaa acgcggatga gccgaatgca cgagaccgcg atggtgctgc tctccggcgg
    11701 ccaggactcg accacctgcc tggcgtgggc gctggagcat ttcggcaacg tcgagaccgt
    11761 cgggttccgc tacggtcagc ggcacgcggt cgagctcgat tgccgtccgg tgatcctcgc
    11821 cgagctgcgc acccggttcc cgggctgggc acgaaagctg ggcgacgacc acgtgctcga
    11881 cctcggcgtg atccccgcgc tcggcgccac ggcaatgacg cacgacatcg ccatcgagac
    11941 cggccgctcg ggcctgccga acacgttcgt cccgggacgc aacctgctgt ttttcaccgc
    12001 ggcggcggcg ctcgcgtacc ggcgcggcgt gcgcacgctc gtcggcggca tgtgcgagac
    12061 ggactattcg ggctatcccg attgccgcga cgacacgctg aaggcgctgc aggtcgcgct
    12121 cggcctcggg atggagcgcc gcttcgtcat cgagacgccg ctcatgtgga tcgacaaggc
    12181 gcagacgtgg tcgctcgcgc gcgcgctcgg cggcgagccg ctgctggagc tcatcgtcga
    12241 gcacacgcac agctgctatc tcggcgagcg cagcgtgcgg cacccctggg gctacggctg
    12301 cggcggctgc ccggcgtgcg agctgcggcg cggaggctgg gagcgctatc gggcgtcgtc
    12361 gtagcgcccg gatcccgcgg cgggcctgcg gctcacccac gcgacgaaac gctgcatgaa
    12421 ctcggggacc cgcgcgtaga tccaggcggc caccgcggcg ttggcgagcg ccgtcgcgag
    12481 gaacagctgc gggatcgaca ggccggccgc gaacagcgcc atcgaggcga gcgccgacgc
    12541 caccatgaag gccgcgttga ggatgttgtt cgccgcgatg acgcgcgact ggtgcgtctt
    12601 ctcgctgcgc gtctggatga gcgcgtagag cggcacgatg tagaacccgc cgaacacgcc
    12661 cagaagcgcg aggtcggcga gcaggcgcca gtgcgagggc tctcgcagga agtgcgtgaa
    12721 atgcgcgcct gcggccgccg cctgcgagcc gctcgcgaag tagagatcga cgccgaacag
    12781 cgtcagtccg atcgtgccga aagggaccaa cccgatctcg acgcggccgc ccgagagcct
    12841 ctcgcacgcg agcgagccgg cgccgatgcc caccgagaag atcgtgagca gcaggatgaa
    12901 cacgctctcg tcgcccccga gcacgtcctt cgcgtagacc gggaactgcg cgagcagcgt
    12961 tgcgccgtag aaccagaacc acgagatgcc gatgagcgac agccacacca cgcggttgcc
    13021 gtgggcgaag cgcaggttgc gccacgtctc ggcggcgaag ttagggccga cgcgcaggtc
    13081 gggatcgacc ggcggggtgt acgggatgcc gcgactcgcc caccagccga ggaccgccac
    13141 gccgacgacc gtcgtcgagg tcgcgaccgc gccctgcggg atcgccgcga gccaggcgcc
    13201 gaggacctcg ccgagcagga tcgcgacgaa ggtgcccatc tcgatcagtc cgttgccgcc
    13261 gatcagctcc tcggtcctca ggtgctgcgg caggtagccg tacttgaccg ggccgaacac
    13321 cgtcgagtgc acccccatca ggaccagcgc cgtcacgagc tgccacaggc tgcccgccag
    13381 gaagccccac gcggccagga ccatcacggc gatctcgaag atcttcaccg cgcgcgcgat
    13441 gcgcgacttg tcgagcttgt cggcgagctg tccgctcgtc gcggagaaca ggaagaaggg
    13501 caggatgaac agcccgggaa gcagcgtgac gagcattcgc ggatccgcgt cggtcagctg
    13561 ccccgtgcgg aacgtcacca gcgtgaccag cgccgtcttg aagacgttgt cgttgaacgc
    13621 gcccaggaac tgcgtgaaga agagcggccg gaagcgccgc tcgcgcatca attgaaactg
    13681 ggtgctcaat cgatggtcgc gccgacggcc ttcacgactt ctccgtactt cgcgtactcg
    13741 gaccggatca gcgccgtgaa ctcctccgtc gtcgtcgcaa acggcgtgag cccgccgacc
    13801 tttgcgatct tcgcgcgcgt cgccgggtcg cccagcaacc ggttggtctc ggcgtgaagg
    13861 cgcgtgacga tcgccgcagg gacttcgccc ccggtgaaca gcccgagcca cgcggtgacc
    13921 tcgaggcccg gatagaactc cgacagcgtc ggcaggtcgg ggaaggtctc cgagcggtgc
    13981 ttgcccgcga ctgcgaggcc tcgcagcttg cccgccttga tctggccggt gacggaattt
    14041 ccgccgaaca tgaccggcac ttctcccgcg atcaccgcga gcgtcgccgg cccgccgcct
    14101 ttgaacggga cgtggacgag gttgatgccg gcgcgcgcct tgagcatctc catcgtcagg
    14161 tggtgctggc tgccgttgcc gatagaggcg tacgcgagcg gcgggctggc gcggcgcgcg
    14221 tactcgatga actcgggcag cgtgtgcacc ggcagcgacg ggttgaccga gagcaccatc
    14281 tgggtggtca cgagggaggc gaccggcgcc aggtccttca gcgtgtcgac cggcatgttg
    14341 cggtagaggt gcgggctgat gacgatctgg ctgtcctgcc cgaggagcag cgtatagccg
    14401 tcgggcgcgg ccttggccgc gagctcgctc gagagattgc cgttcgatcc cggccggttc
    14461 tcgaccacca ccggctggcc gagcgcctcg ccgaggcccg gcgcgatcac gcgcgcgata
    14521 gtgtcggtcg cgccgccggg cgggttgggc acgaggagcc ggagcggccg cgtcgggtac
    14581 tgcgccgacg ccgatgccgc gatcagcagg atcgccgcgg cgcacagcgt tcgcgacgcg
    14641 agaacgagag caagagctcg ggtcatgtcc acctccttgc gccattttgg ccgcaaaccc
    14701 cgccgcgggt ccagccggcg acgcacggcg gctccgtcgg aactagaatg gccggctgcc
    14761 aacgatccag tgggagccgc gatgaaaatc tcgatgtacc aggcgagcgc gccgcgcttc
    14821 gtcaacatgc tgaccaacct ttcggccatc ctcgacaagg cccaggccca tgccgaggcg
    14881 aggaagatcg acccggcgac gctcgcgaac gaccggctct tccccgacat gttcccgatg
    14941 aagcgccagg ttcagatcgc gtgcgacacc gccaagggcg ccgtcgcacg cctcgccggc
    15001 gtcgacgtgc caaagcacga ggacaccgag cagacctttc ccgagctgaa ggcgcgtatc
    15061 gcgaagacga tcgacttcgt caagagcatc cccccggcgc agctcgaagg caccgaggac
    15121 aagaacatcc atctcaagct cggtccgcgc gaggtcgact tcaccggcat gcaatacctg
    15181 ctcgggcacg cgctgccgaa tttctatttc cacgtgacca cggcgtacga catcctccgc
    15241 cacaacggcg tcgagctcgc caagcgcgac tacatcggca cgccgtgatc gggcgcccgg
    15301 cggtcgatcc ggaagagcca tgaaccgcag agccttcctc gctgccggcg gcgcgctcgt
    15361 gctcggcggc tgcgcgacgc cgcgcctggg cgagtacgag cccacggtcg tcgaggcccc
    15421 gcaatggcgc gcgggcgaca gctggacctt tcggcgcatc gacggctaca acggcctgcc
    15481 gcgcgacctc ctcacgcgca cggtgagcgc gacgagcccg gcgggcaccc gcttcgtcac
    15541 cgtggatcag aacggcgcga cgcgcgacga cgccctgttc gaggcacccg gtgtgcaggt
    15601 ctcggggctc ctcagcgagg acggcccgat cgcgggtctg ttcgagccgc ggctcgtggt
    15661 ctacgacttt ccgctccatt ccggcaagga atggcgccag ggcctctacc ggatttacgg
    15721 cagcggcttc cgctcctatc tcagccacgt gtcgcgcgcc gaaggctggg aggacatcgc
    15781 cgtcggcgcg cgcacttacc gcgcgatcct catccgcagg accttcaatt tcggggcgcg
    15841 cgattcgttc actccccgac cgctggttca cgacgagacc gagtggtacg tccccgagct
    15901 gcgcgggacg gccagcctgc agacgaacga atggctgcag gcgccgccgg gcggcttcag
    15961 ctggccggga taccgctacg tatacacgct cgactctttc cgccttgtct gaagcgccgg
    16021 gctcgtttgc gctgaacgtt gcgcgccgcg cgcgtgtcct acgagcgccg gcacgagcgc
    16081 gcgccggcga ttccacgaat ctttttcagg agaccgaggt gaacaaggac caggtgaagg
    16141 gcagcgcgaa gcggctcgcg ggcaaggcgc aggaaaaaac gggcaagctg accaagaacc
    16201 gcaagcagca ggcgaagggc ctcgccaagc aggcagcggg gaaggtgcag aaggcgtacg
    16261 gcgacgcacg cgacgacctc gacaaggacc gggaggattc cggccgcgac ggctagcgag
    16321 ccgccgctcc tccgacagcc gcgccacgcg gctgtttttt ttggcgggta agatacggct
    16381 gccgcgggta gtcgccgcgt cccgggcgtg gtcgagcgcc cactaaccag gagatcagca
    16441 tgaccgcatg gcgttttccg cggcggtggg ccgtcgcgtt gctcgttctc gcggtgccgg
    16501 ccggtgcact ggcgcagacc gcggccagca gccagttcca gccggaggtc gggcagcagg
    16561 gcaaggacgt cgtctgggtg ccgacgcccg aggcgctggt gcagaagatg ctcgacatgg
    16621 cgaaggtcac tcccaaggac ttcgtgatgg acctcggctc gggggacggc cgcaccgtca
    16681 tcggcgcggc caagcggggg gcgcacgcgc tcggcatcga gtacaacccc gacatggtcg
    16741 agctgtcgcg ccgcaacgcg aagcaggccg gcgtcacgga tcgggccaag ttcgagcgcg
    16801 ccgatctgtt caagaccgac ctgtcccggg ccacggtgat cacgatgttc ctgctcaccg
    16861 acatcaacct gcggctgcgg ccgacgatcc ttaatctgaa gcccggcacg cgcgtcgtct
    16921 ccaacacgtt ccgcatgggc gattgggatc cggacgagac ggccgaactc ggctgcagct
    16981 cgtactgcac cgcgtacctg tggatcgtcc cggcgcacgt cgagggctcg tggaagacgg
    17041 cccagggcga gttgacgctg cggcagacgt accagacggt gagcggcaca ctgagctcgg
    17101 gcggcaagtc gctgccggtc cagcgcggca agctgcgcgg cgaccagatc agcttcaccg
    17161 ccgggggcgc cgagtaccgc gggcgcgtcg gtgacaagat gatcgagggc accgtcaaat
    17221 ccggcgcgaa gagcgagcgc tggagcgcga accgctgagc cgcgccatgc gtccactgct
    17281 cttcgccgcc gccatcgctg cggcgctcgc cacgggcgcc gccggcgccg ccgatgcaga
    17341 cgccgtccgc gtcaacgcct tccccaatgc gaagtcgctg cccctccatg ccggcgtgga
    17401 gaagggcatc ttcgcggcgc gcgggctcgc ggtacggctc tcgttcaccg aaaactccac
    17461 caagcagcgc cagggcctgg ccgcgggcga gttcgacgtc gtgcacgcgg cggtcgacaa
    17521 cgcggtggcg atggccgacg gcggccgcga cgtcgtcatc gtcaccggcg gcgacagcgg
    17581 catgaaccag ttcttcgtgc agccgggcat cggctccttc gccgacctgc gcggccgcgt
    17641 gcttgtcgtc gacgcgccga acaccgcgta cgcgctgctc gcgaagaaga tgctgcagaa
    17701 gcacggcctg ctcgaaggcc gcgattacac cgtgaagcct gtcggccgcg gcgagctgcg
    17761 cctgaaggcg atggccgcgg atccggccaa cgcggcggcg atcctcaatc tcccgtatac
    17821 gatccaggcc gaggcgatgg ggatgaagag cctcggcaac ccggtcgacg cgctaggacc
    17881 gtaccaggcg aacggcgcct tcgccctgcg cgcctgggcc gagcgcaatc gcggcctgct
    17941 cgagcgctac atcgccgcgt acgtcgagtc gctgcgctgg gtgctcgacc cgcgccaccg
    18001 cgacgagtgc atcgcgatgc tccagcgcga gctcaagatc acgccggagg tggccgcccg
    18061 cgcttaccgg cagctcgccg atcccgcggc agggttcacg cccgacgcca ggttcgatat
    18121 gcaggggttc cgcaacatgc tggcgctgcg ccaggagatc gaaggaggcg gccgcccggc
    18181 gcccgcgccc gagcgctacg tcgacctgtc gtactacgag cgcgcgctgg cgaccttgtc
    18241 tcgctgaggc gacgcgaagc gcaggacctg ctcgagcacg ttggcgccgg tctggcgcgg
    18301 cggcagcacg tcccagagct cggcgcgcgg catgagctcc ttgagcgccc acgccgagga
    18361 caccgtgtgg cgcgtgtcgg ctcccgacag gatgagcgcg ggcatgtcca tgcgcatcag
    18421 ctcgtcgccg ctcgcgcccg agggcatggt gtcgttgaag atcgcgtcgc ggctctgcgc
    18481 gcagatctcg aggtagcgcc cgacgtcctg gcgcgcgagc tcggcggcaa actccagata
    18541 gaccgcggcc ggcgagcccc agggcccgat ctcggggtcg taccagaaat tctcgccttt
    18601 cggcgcgcgc gcgacgaccg ccgcgaggcc gttcgcgcgc acgaaatcga tgtgtcgctg
    18661 gaagaagccg tggcccttct tcatccagag gtagccgccg actggccaat gcagcagcag
    18721 gccgaggcag gcctgcgggt ggcgggccgc gaacgcgagc gcgagcgacg cgcccatgca
    18781 cgagccgagc acccaggcgc gcttcgcgcc ggcgagatcg agcagtgcct tcgcctcggc
    18841 gacgtagaga tcccaggcga gcggctcgac gcgcccgccc gacaggccgc actcgcggcg
    18901 gtcgtaggcg atcgtggtga agtggcgcga gagcgtcgcg aggccgtcca tctcgccgaa
    18961 cgcctccttg ccgcccgcgg ccgtccagcg cgacatcacc gagcgccagc cgcccggagc
    19021 gaacatcagc aggtgcggtc ccgcgccctg gacggcgtaa tggatgcgga tcccgttgat
    19081 cgtcgcgaac ggcatgccgc tattatagag acgcgcaatt catcaggcgg agagcctatg
    19141 gaatcgaaat ggaagcacca gggcgtgcgc gtcgtgcgct ccggcgagct cgacgtgaac
    19201 acggcgcaga cgccgggcat gaaccgcgcc gcggcgatca cgtacgcgcg cgcgggcgcc
    19261 gagaagctct gggccgggac cgtcgtgatc cacccgaaag cgaagaccgg cgcgcatcat
    19321 cacgggccgg tcgagagcgt catctatgtc gtgagcgggc gcgcgcgcat gcgctggggc
    19381 gagaggctcg agttcgtcgc cgaggcgggc cccggagatt tcatctacgt gccgccgtac
    19441 gtgccgcacc aggagatcaa cgcgagcgac gagcagccgc tctcgtgcgt gctcgtgcgc
    19501 agcggccagc agccggtggt cgtgaacctg gacatccccg cggtggagca acccgagcag
    19561 gtgcactggg tcgacaacat tcatcccgcg ccgaaggcgt agcgtcttcg tcgccggatc
    19621 gccggacgcg ctgtcggtgc gcaacgacag acggcgcact cttgcagaac catcggcaaa
    19681 gttgtccatt cgccagtgag cgggctcttt ccgacacccg gaggacgggt gaaagggcct
    19741 cccggcgcgc gtttttccgt atcgattcgt catattcttc gccttccgaa acagcccctt
    19801 agcggcccga catgggccgc gtcccgcggc agcccacccc cgtccgggcc atcgccgtca
    19861 aattcaggcg tattcgtgga acggagcttg ctcgttaagc gaccgtaagg atcggaatcg
    19921 ggaggcgcgt gaaatacgtc acgcctccgc gttcgggacg ctcgaaagga gacgacgatg
    19981 aaaacgctcc aaatcgaaca gctgagccct cagctccgcc gggtcgagga gggtttcgcg
    20041 gcgctggtgc acgtgctgtt ccgcacgtac ccggagctgc tgtgcttctc cctgggcggg
    20101 cgcgccgatc tgccgccggg catggacatc ggcgacaccg aggcgcagat cttcatcacc
    20161 gacgtcgatt tcggcaagcc catcagccag gaataccaga acgagatcgg cgaggccctc
    20221 ggcggcatcg tgtccgacat cgtgaccgag cgtccggaag cgctcgaagt gctccccgga
    20281 aggaccttcg cccgcacgct gcagtagctt ctgctacggg ctccccagga attcgcgcat
    20341 ccaccgacga tagtcgctgt cgcgcgtcgc ccgtttcacg aggtcggcgg cggccacgcc
    20401 ggtgatctgc gcgggcggcg ttccctcccg cagcgccttc agcgtctcgt gcaccgcgcg
    20461 caccgcggcc ataaaaggct ggtgcccctg caggcacacg cgcacgccgc ggcttcccag
    20521 gtaatcgcga tccatcagct ccggcccgac gttgccgagg aagatcggca cgcccacgct
    20581 cggcgcgatc gcgtcgagct gcgcccgcga tttcatgccc acgaagaaca gcgcgtcgac
    20641 tccggcggcc tcgtacgctt tcgctcgcgc gacggcctcc tcgacgccgg cgaacggcag
    20701 cgcgctggtc cggccgacga cgaccagcgc cgggtcgccg cgcgccgaca gcgcggcgcg
    20761 catcttgccg gtcgcctccg cggtcgggat gagctggtcc ttgccgccgc cgaagggtgc
    20821 gggcaggagc gtatcttcga tcgtgagcgc ggccacgccc gcgttctcca gctcctcgac
    20881 ggtgcggcgc acgttgagcg cgttgccgta gccgtggtcg gcgtcgacga tcagcggcag
    20941 cgtgccggcg cggcagatcc ggcgcgcctg ctcggcgaac tcggtcagcg tcagcacgac
    21001 gaggtccggg gcgccgagca cggcgagcga cgcgatcgac ccggcgaaca tgccggcctc
    21061 gaacccgagg tcctccgcga tacgggccga gagcgcgtcg tacacggaac ccggatagat
    21121 acagcgcgtc cctgacaaga gggcgcgcag gcgctggcgt cgttcgctga agctcatcga
    21181 gggtccgatg cggaagaacc atcgcagatt atcatgagcg ctggctcaat gagccgaacg
    21241 gcccgtggcc cggtgcatgc agccgccatc gcggttcgcg gccccttcat agaatccacc
    21301 ggtcgcgcgc ctagaccgga aaaatgctcc ccacctcgaa gacgaaccga cccgcagacc
    21361 tcttcgccgg gaagccgtcg tcgttccagg ccgtgctcgc cgaagtcgag gagttctgga
    21421 cgccgctgcc cgacaagccg gaggagaccg cagaagccgt cgcgcgggcc ttgtggctca
    21481 ccgccgcggg gacgcccatg tccgccgagc gctgcgtcgc cctggcgctc cccgcgctcg
    21541 acgaacaggc gctcgggcgc ctgcggacgc tgctcgagcg caagcgcgac ggagagccgc
    21601 tcgcgcatct caccggtcgg cagacgttca tgggcctcga cctgctcgcc ggtccgtcgg
    21661 cgctcatccc gcgcaaggaa accgagatcc tgggacgcgc gctcgccggc aagctcgatc
    21721 agatcgcgcg cgagcgcgac gagatccgcg tgatcgacct gtgcaccggc tgcggcaacc
    21781 tcgcgctcgc ctgcgcgagc cacgagccgc gcgcgcgcgt ctggggcgcg gatctgtctc
    21841 ccgaggcggt cgcgctcgcg cggctcaatg ccgagcgctg cgggctcgcg tcgcgcgtcg
    21901 agttccggga aggcgacctg ttcgcgccgt tccaggacgc ggagttcctc ggccggtgcg
    21961 acctcgtgtc gtgcaatcct ccttacatct cgtcctcgaa agtgcccggg atgcaccgcg
    22021 aaatctcggg cttcgagccg gggctggcgt tcgacggcgg aacgttcggc atctcggtgc
    22081 tcacgcgctt cctgcgcgag gcgccgcgct tcctgaagcc gggcgcgtgg ctcgcgctgg
    22141 agatcggcgc ggggcagggc ccggcgaccg cacgtcagct cgcgaagaac cctgcgctcg
    22201 cggaggtcga gactcgcagc gacgccggcg gggagatccg cgcggtgctc gcccgcgcgc
    22261 gctgacgcgg ggttcgcctc cggagccgcg ccgccgcccg gcgggttacc attgccgtcc
    22321 gcgcgaggat ccgggacggt catgagaacc atagcgatcg aggagcacta caccaccgcg
    22381 atgtaccggc agcaggtgcc ggcgaacgag ttccgcaact tctacctgac ctcccgcagc
    22441 gagcagctcg ggcacgacat cgtggacgag ctgggcgacc tcggcgcccg gcggatcgcc
    22501 gccatggacg ccgccggcat cgacgtgcag gtgctctcgt tcggctcgcc cggtccgcaa
    22561 gggttcgcga aagagatggc gatcccgatg gcgcgcgacg cgaacgatcg ggtgcacgag
    22621 gcgatgcagg cgcatccggc ccgcttcgag ggattcgccg cgctgccgac cgccgacccc
    22681 gcggcggcag ccgacgagct cgagcgctgc gtgcgaaagc tcggcttccg cggcgcgatg
    22741 atccacggcc accagcaggg agagttcctc gacgaccgca agtactggcc ggtgttcgag
    22801 cgcgccgagg cgctcggtgt gccggtctac ctgcacccga cgctcgcgca tcccaaggcg
    22861 ctcgagtcct acttcaaagg ctacgaggag ctggcgcgcg ccgcatgggg cttcgcgatc
    22921 gacacgagct gccacttcct gcgcatcgtg ttcgcgggcg tgttcgacgc gtacccgcgc
    22981 ctgaggatca tcctcgggca cctcggcgaa ggcctgccgt tcgcgatgca ccgtctggac
    23041 gaccacgccg gccgcgccgc ggcgcgccgg ggcctgaaga agcgcccgat cgagtatctg
    23101 cgcgataacc ttctcgtcac gaccagcggc aactggtacg agccggcgtt cgtgtgcacg
    23161 ctgctcgcgc tcggcgcgga caacatcctg ttcgccgcgg actggccgta cgaggcgaac
    23221 ctcacggcgg cggcgtttct cgggaagatc tcgatcagcg agggcgaccg cgagaagatc
    23281 gccttccgca acgccgagcg cctgctcggg atccggggcg cgtcatgagc ctgtccgagg
    23341 tgcgcgaagg catgaggatc gaatgggacg tgccgatcac gatggacgac ggcctggtgc
    23401 tgcgcgccga cgtgttccgg cccgaggccg agggacgcta tccggtgatc ctgagctacg
    23461 gtccgtacgg caagggcctc gccttccagg acggctacaa gacggcgtgg gagatcatgg
    23521 cgcgcgacta ccccgacgcc gtcgccggct cgtccaaccg acacgcgaac tgggaggtcg
    23581 tcgatcccga gaagtgggtg cccgacggct acgcctgcgt ccgcgtcgac tcgcgcggcg
    23641 ccgggcgctc gccgggattc ctgtgccaca acaacgctcg cgaaacgcgc gacatctacc
    23701 tctgcatcga gtgggccgcg caacaggcct ggtgcagcgg caaggtcggc atgaacggca
    23761 tctcgtatta cgcgagcaac cagtggcgcg ccgccgcggc gaacccgccg cacctcgccg
    23821 ccatctgcgt ctgggaaggc tggaacgacg cctaccgcga gagcgcgcgc cacggcggga
    23881 tcatctgctc gttccgcaag aactggcagg acatgcaggt gaagaccgtg cagcacgggc
    23941 agggcacgcg cggcgcgcgg agccgcgtca cgggcgagct ggtctgcggg cccgagacgc
    24001 tgccgcccga cgagctcgtc aagaaccgcg aggacatgtg gcaggcgttc ctgtcgcgcg
    24061 agatgtggga ccgctattac gcggagcgct cgcccgacct tgcgcgggtc aacgtgccgc
    24121 tgctctcggc ggcgaactgg ggcggccagg ggctgcactc gcgcggcaac ttcgagggct
    24181 tcatgttcgc agggaccaag cagaagtggc tcgaggtgca cggcggctcg cattgggcgc
    24241 cgttctacac cgactacggc gtgaagctgc agaagcgctt tttcgcccat ttcctgaagg
    24301 aggagaagaa cggctgggag cggcagccgc gcgtgctgct caacgtgcgt catcccggcg
    24361 agaagtacgt gctgcgcgag gagaacgaat ggccgctcgc gcgcacgaag tggacgcgct
    24421 tccatctcga tccgaagggc atgcgcctgg gaacgggcga ggtcggggca gtgtcgtcgc
    24481 tcgcctacga cgcgctgggc gacggcctca ccttctcgac gccgccgctc gcgaagccga
    24541 tggagatcac cgggccttca tcgctcaagc tctcgctgtc ttcgtcgacc gcggacgccg
    24601 acgtgttcgc cgtgctgcgc gtgttcgacc cgaagggcag ggaggtgctg ttccagggcg
    24661 cgctcgatcc gaagacgccg gtggcgcagg gctggctgcg cgcctcccac cgcaagctcg
    24721 atccgcggcg ctcgctgccg taccgtccgt tccacacgca cgaccagaag cagcccctcg
    24781 agcccggcaa ggtgtacgag ctcgaggtcg agatctggcc cacctgcatc gtggtgcctg
    24841 ccggctaccg cattgcgctg tccatccgtg gccgcgacta cgagcacgac gaggcgccgg
    24901 cgacgctctc gaacatgaag aacccgatgc gcgggtgcgg gccgttcacc cacgacgatg
    24961 agaccgaccg gccgccgaag gtcttcggcg gcaaggtcac gctgcacttc ggcgccggcc
    25021 gccccgcttc gctgctgctg cccgtgatcc cctagcgctg ccggcgcgca ccggcaccac
    25081 cgcggtcgcc tcgagctcga tcttgccggg ggagtcgagc aggtcggcga cgaagaccat
    25141 gctcatcgcc ggatagtggc gtcccatgtg acgccgccag atcgcgccca gctccttgcg
    25201 cgcgcgcagg tagcggggct tgtcgcagca gtacgcggtc atgcggcaga cgtgctcggg
    25261 ccgcgcgccc gcctcggcga gcaccgcgag cacgttccgg agcgcctgct cgaactgcgg
    25321 tgcgatgtct tcggattgga actgctggcg ctcgtcccag ccgacctggc cggcgacgaa
    25381 gacgatgcgt cccgaggcgg cgatgccgtt ggcgtagccg atcggcgcgg cccagccctt
    25441 cgggtgcagc acgtccacgc gagccctcaa tccgagaaca ccggcgggtc gggatcgtgc
    25501 tgccagtcct tgtcgggcgc cggcgtgcgc gagatccatt cttcgatcag ctcaatgtgc
    25561 tcggcttctt cttccgccat ctcgcgcgcg gccttccgca ccgcgggcag cggggcgctg
    25621 cgcgccactt cggcgaaata cgcgcgtgcg cggcgttcgc acgcgagcgc gacctggagc
    25681 gcgtggtagg gctgcatccg gtaatggaga tcgcccgggt cggcggtctc ggggccttcc
    25741 ctgcccgcgc cccagcgcac gcctccggga ggcagcaccg gcgggtgcgt cgcgaggctg
    25801 tgctcgagga tctggtcggc gtgcatctgc tcgatccgcg cgagcttgcg gaagagctcc
    25861 gcgacctcgg gattgttgtg ggtctgcatc gcgtcggcga acgtgaggta gcgctcggcg
    25921 gcctcgacct ccattgtcca ggcgcgcgcg aggaaatcgg cctggtcgct tgcgcgggcc
    25981 cgcccggcgg ccttgcggcg tgtggccttg cgcgtcgcct tcggggcagg gcgggcgctc
    26041 acagggcgaa gtccgactcg agatcggctg gacgcttcac cgccgcgccc gccgagcgcg
    26101 cgaacggctt gcggttgccc ttgtagagca ccccgatgtt gaagccgtcg tcggtcatga
    26161 tgcggcgcgc ggcgcgcgcc gcatcgtccg tgaccgcgac cggcgcggcg cgcaccttgt
    26221 ccttccattc gcgctgctcg ggacggaacg tcacgcaagg gctcaggatc tcgacgaacg
    26281 agaaccccgg cgtgcggatc gcgtcggcga tgatctcggc cgtgccgttc gggtcgccgg
    26341 cgaaggcgcg cgcgacgaag ttcgctccgg ccgcgagcgc gatcacgagc gggtggaagc
    26401 tgcgcagtcc ggtgccgccg ggcgagagct tggagtccca gtcgggctcg gtggtcggcg
    26461 acggctgtcc cttggtcatg ccatagacgt ggttgtccat gacgatgtac gtgatatcga
    26521 cattgcgccg gcaggcgtgc aggaaatggt tgccgccgat cgagtagccg tcgccgtcgc
    26581 cgctggcgac catgaccgtg aggtcgggcc gcgcgatctt gagacccgtg cccgccgcga
    26641 gcgcgcgccc gtgcacgccg tggaagccgt agcagttggt gtacgccggg atgcgcgacg
    26701 agcagccgat cccggacacg accgcgacgt tctcggggcg cagcgcgagc atcgcgaacg
    26761 ccttggtgat cgaggacagc accgagtagt cgccgcaccc cgggcaccag atgggcttgt
    26821 agtcggactt gaacgcctgc gccgtcggcg cggcggaggg gacgctgctc atgcgcggct
    26881 ccattcgacg agttcgcggt agacctcgcc cggcgcgatc ggcaacggac cgggacggtg
    26941 caggctgcgc acctcgccgg gaagatcgaa gtccgagcgc aggtagcggt ggaactgcgc
    27001 gccatggttc tgctcgacga cgagcacgcg cttcacgccg gcgagcgcct gcgcgagacg
    27061 ctcgggctgc gcgggcgaga gcaggcgcat cgagacgagg cgcgccttga ggccgtcctg
    27121 tgcggcgcgc gccagcgctt cccgcacagg acccgtgcac gagccgaacg tgatcaccgc
    27181 cgtgtccccg tcgccctcga tctcggccca cagcgcgccg tagtcgtgtt gcttcagctt
    27241 gcgctcgcgc ttggcgagct gcgccgcgtg gtcggccgac tgcgacgagg gaatgccgcg
    27301 ctcgttgtgc tcgagaccgt cggcggtata cgcgcagccc ggcgtaccgg gaaccgccat
    27361 gggcgagatg cccgattcgg tcagcgcgta gcgcttgtag ccgtccgcgc gcgccgcgga
    27421 gacggctcgc tcgccgatga agctcatgtc ggcaggccgg tcgaccgtcg cgcgcgtctg
    27481 cccgaggtac tggtcggaca gcacgatcgc gggcgcctgc atggcctcgg ccaggtgcac
    27541 ggcccattgc gtcgcggtga ggcagtcggc cacggagttc ggcgcaacca cgacccgcgg
    27601 cgcgtcgccg tgcaggccct tgaccgcgat gttgacgtcg ctctgctcgc tcttcgccgg
    27661 gataccggtc gacggaccgc cgcgcatcac gtcgacgacg acgatcggca cctcggccgc
    27721 gaccgcgagg ccgagcgcct cggtcatcag cgcgaggccg gggcccgagg tcgcggtgag
    27781 cgaaggcacg ccgccgaacg aggcgccgat cgccatgttg atcgaggcga gctcgtcctc
    27841 ggcctgcacc agagcgccgc cgacctcggc gagcgcgggc gccatccact ccagcacctc
    27901 ggtcgccggc gtgatcggat aggcggcgac gaagcgcact ccgccgcgca gtgccccgta
    27961 gcccgcggcc tcgttgccgc tgatcagcca gcgcggcgcg gcggcgcggc gcggcggcgc
    28021 gagccgcggc acgccgggca gctgcgtcgc ggccgcatac ccggcgtcga cggcggcgag
    28081 cgtcgccgcg agcgcgtcct gtcccctctt ctgcgattgt ctgagcaccg ccttgaccgc
    28141 gtcggcggga agaccgacca gcgcgcccgc gacgccgacc atcaccatgt tgggccagct
    28201 tccgggaatc gacttcgccg tcttcttgaa cgcgaccgac gcgcggcgcg gcgcgcgctt
    28261 gacgaaaacc tcgggcggct cgccctggtc ggggtcgccg accatcaggc tcgcgccgtc
    28321 gatgggaatt tcgtcggcga agcggtggac gttctgccag tcgatcgcaa gcagcgcgtc
    28381 gaagcggtcg tcgaggcagt cgatcgggcg cgccgcgaag cgcagcagcg ccgccgcctc
    28441 gccgccgcgg atctgcgggc cgctcgagcg caccatcagg ccgtaccagc ccgcgcgcgc
    28501 tgcggcctcg agcaggacgt tgccggccgt catcacgccg gagccgccgc tgccggtgag
    28561 cgcgacggaa acgctggatg ccgcggccac ggcgtcggat ctgtcggtca cctgaagtcc
    28621 tccttggtga tcgcagtcta ggcagccgaa accggcccgg gtttgatcta ggacaaaggt
    28681 attgtggtaa aggggtactg taataccgcc attccgttcc ggaggaggcc cgatgaacgc
    28741 tcccgccgct cctttcgtgc tcaagagcca cgacctcgtc gacgggtgga ccgcgcgcgg
    28801 cgtgcgcgtc gagaagcgcg ccgccaggac gcccgacggc cgtaccgtcc cgggtctgta
    28861 caacgcctgg atctggctcg acaacgaggc gcagtacaac tcctatacga ccgagatggc
    28921 caaggggatc gtgctcgcgt tccgggcggc ctcgaactcg cgcgacgtga actgcgtcgt
    28981 cttcaccggc gtcggggaca aggccttctg caccggcggc aacaccaagg agtacgccga
    29041 gtactacgcg gggcgtccgc aggagtaccg gcagtacatg cgcctcttca acgacatggt
    29101 ctcctcgatt ctcgcctgcg acaagccggt gatctgccgc gtgaacggca tgcgcgtcgg
    29161 cggcggccag gagctcggca tggcgtgcga cttctcgatc gcgcaggacc tcgcgcgctt
    29221 cggccaggcg ggcaccaagc acggctcggc gccgatcggc ggcgcgaccg acttcctgcc
    29281 gcccgtcatg ggcgcggacc gcgcgatggt cgcgtgcgtg ctgtgcgagc cgttctcggc
    29341 gcacaaggcc taccactggg gcgtgctcgc cgacatcgcc cccgcgctga aggtcgacgg
    29401 caagtacgtc gccaacccgc tggtcgaaac gcagcgc
//
LOCUS       DATDMW010000108        14987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_31440, whole genome shotgun sequence.
ACCESSION   DATDMW010000108 DATDMW010000000
VERSION     DATDMW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31440"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..693)
                     /locus_tag="VI319_04840"
     CDS             complement(136..693)
                     /locus_tag="VI319_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEY6863205.1"
                     /translation="MDEIVVRSMLRWPDVPSVYGWLALDRRGAWLIKGVSGAFGRITN
                     AAVIDFIGRNYAADGAGRWYFQNGPQRVFVSLAYTPWVWRLDDSGMRLVSHTGADAGA
                     LRAGFLDESGALLLETALGVGVVSDRDLAAVAARVPEPVARGETADCALLGARVRVAP
                     IRSADVASRFGFVPRPAPAPGEPEC"
     gene            707..1156
                     /locus_tag="VI319_04845"
     CDS             707..1156
                     /locus_tag="VI319_04845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016254048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY6863206.1"
                     /translation="MDRGDFTFFHTLRVRWAEVDRQDVVFNGHYFLYFDVAIAEYWRA
                     IGFRYPDDVLAHGTDIYAVKAAAEYHASATYEDVLDVGCRVARIGRSSMTFAFGIWRG
                     GDRITSGELVYVNADPKTRKSAPWPEALRSAVLRYERARPDVASNRD"
     gene            complement(1162..2196)
                     /locus_tag="VI319_04850"
     CDS             complement(1162..2196)
                     /locus_tag="VI319_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="depolymerase"
                     /protein_id="HEY6863207.1"
                     /translation="MPGMLARLLLALVLLLASPARAAERLPALGADGAAVTVSGVSSG
                     AFMAVQFHVAHSATVRGAGAIAGGPYDCAQGSLWTALTSCMDPGPFSPLPDARRLAEL
                     ARGLAKAGGIDPVERLAGAKLWLFTGTRDDTVSSEVVEALRRFYLALAPDADVAFENS
                     VPAGHGMPTQEEGNECSTTQPPFIVDCAYDAAGILLAQLLGPLAAPTPKQDGKLLEFD
                     QREFAGGNPAAISMGDAGYVFVPDACAKERCRVHVVFHGCRQSATQIDKRFVLEAGYN
                     RWAAANRLIVLYPQTTPRWGYSFASGNFVFNPKACWDWWGYTGARYAAKEGAQVRAVQ
                     AMLERLAQPR"
     gene            2251..3696
                     /locus_tag="VI319_04855"
     CDS             2251..3696
                     /locus_tag="VI319_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6863208.1"
                     /translation="MQNRDQLYVDGRWTASAGANTIAVVSPSTEEPIGRVPEGTPDDV
                     DSAATAARAAFDSWAATPPAGRAAFLSKIHEGLKARADEIGRTIAAEVGMPLKLATRI
                     QAGSPIYTFAMYAKMLGSFAFEERVGNSIVLREPVGVVGAITPWNYPLHQIAAKVAPA
                     LAAGCTVVLKPSELAPLNAFILAEVVHAAGLPKGVFNLVTGYGPVVGEAIARHPGVDM
                     VSFTGSVRAGKRVAELGAQQVKRVTLELGGKSAAIVLDDADLAQAVKSTVNACFLNTG
                     QTCSAHTRLLVPESRYEEAARLAVEAAQGFTVGDPFGDAKLGPVTSKAQLERVRGYIA
                     KGVAEGAQLLTGGAERPDGLARGWYVRPTVFGRVDPHSTLAQEEIFGPVLAIITYRDD
                     DEAVRIANDTIYGLAGGVWSKDEERAKRVARRLRTGQVDINGGAFNMQAPFGGYKQSG
                     RGRELGRPGLEEYLEYKSLQLKAELRSESRS"
     gene            3712..4443
                     /locus_tag="VI319_04860"
     CDS             3712..4443
                     /locus_tag="VI319_04860"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEY6863209.1"
                     /translation="MILSHKVHVLTRKEELDTVRLPGKVVIVLDVLFATSTIVAALAA
                     GATEVIPTLDGAAARAVAAREDAGGFVLAGELNTVTLEGFAPPTPLALIEHGVAGRKL
                     IYSTTNGTVAFHACSGAQDVYAGALLNGEAVVDRVVSAHPDRTLLIVCSGSAGNFNLE
                     DFYGAGYLVELLASRLGDGADFSDAARAARALYRSGAPEETLLACRVGRMMIVRGLDR
                     EIRYAAQRSVARVVPRYDGAALRPC"
     gene            4479..5447
                     /locus_tag="VI319_04865"
     CDS             4479..5447
                     /locus_tag="VI319_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863210.1"
                     /translation="MKLIFAALVALAAAGGVSAQESAADYPSRAVRLVVPNAPGSSID
                     TMSRIVAAKLGETLGQSVFVENRDGAGGLIGVEAARSAKPDGYTLLCASNGSMMIAPL
                     LKKPVPYDALNDFTLIGPFAVTPNVLVVNPELPVKTVKELIEYARTHASTINMASAGV
                     GSQSHLSGVLLMTMAGFDSLHVPHKGGGPSVNSVVAGQTHWTLTPAPAAISFVRNGKL
                     RALGQSLPHRSSILGDMPAISETVPGYDYSGWAGLVAPKGVPAPVVDKLRDALTKTLS
                     LADVKEGLARQGAEELTGTPDEFRRFIERDHANTVKVIRAAKLQSE"
     gene            5455..6117
                     /locus_tag="VI319_04870"
     CDS             5455..6117
                     /locus_tag="VI319_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863211.1"
                     /translation="MDLLPPSALLESPLAIAGLALGAAGAILVLAGMIALVRMRPVRF
                     ALRTLAGLLLLSLAGLAGAVLLGVQGYRALTREDVAARVSVRPTGPQSFAATVRTADG
                     RQASYALAGDEIYIDARILKWKPVANVLGLHTEYELDRVAGRYRDVEQERSAPRSVYA
                     LGTEKPVDLFSLRRRYAFLAPLLDAEYGSATFVPVTEPAELELRVSTTGLLIRDATPP
                     RP"
     gene            complement(6130..7125)
                     /locus_tag="VI319_04875"
     CDS             complement(6130..7125)
                     /locus_tag="VI319_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863212.1"
                     /translation="MKQTRASIAAALALAAASCCAMAQQSRGGAAAGDYPARPVHIMV
                     GFTPGGGPDITARYIAQKLGETWKQQVLVDNRPGAGGTIAANAVAKAAPDGYTLLSVS
                     SAHAVAPAIYRTLPYDTFKDLAGITQTASSSYVLVVAPSLGLRSVKELIARAKAAPGQ
                     LNFSSAGVGSGTHFAGEMLKAMAGIDVVHVPFKGIPEALTETTTGRVQFFMAPIANSV
                     NLVRDGKLVGLAVSSRERDPLLPDLPTLSEAGVPGYESILWFGLLTSSAVPRPIVARL
                     NRDIVRVLQDPETRARWAPIGMTPRPMAPEAFDALIRHDEAVFSRIARAASIRAD"
     gene            7343..9016
                     /locus_tag="VI319_04880"
     CDS             7343..9016
                     /locus_tag="VI319_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863213.1"
                     /translation="MTLHIAGLARFLAVLAVCGAGLAAAQDPEENVPPVPTFAELEAA
                     GAVIGEIHVDSEDIFDLTDPREDKPLYSLANWLHIRTRPDVIRRQLLFASGERVSARL
                     IDETERNLRGNDYFYDVAIRPLDVHDGVVDIQVKTRDTWTLVPTANLSRSGGVNRGGL
                     GFRDSNFLGTGTRFSIGRKASSDNTGNTAPASLDLQLSQANAFDGHTALSYNLSRFAD
                     GRHDGLSVARPFYALDTRWSAGASGARDDRTIFSFVGANTAVPIYRRREDTASVAGGW
                     SPGLADGWTHRYSLGLSWLSDNYSPAADAAPDAQIPTDRTLVSPFLQYEAVQDDFRKV
                     VNRDLIERTEIFAMGLHANAQFGRSLPQLGSTQYVSMYSGGLSKGFDVGSSGTLLASG
                     SVSGEYGDHHNDRQVGNTTFKYYLAQSASAVFFASFAADRLRFKDGSQQLLLGGDNGL
                     RGYPAHYQSGDRRVLFTAEQRLYTDWFPYRLFRVGGAIFLDAGRAWSGPLDEEPANRR
                     WLADVGFGIRVLNARSSSGTTVHIDFAFPLHREDGIKSYQFSFMSKTGF"
     gene            9086..10735
                     /locus_tag="VI319_04885"
     CDS             9086..10735
                     /locus_tag="VI319_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863214.1"
                     /translation="MSRPLAGWALLLACIAAPAWSQADPDAARLPSFGELEAAGAVIG
                     EIRIDNQNVFDERDARENNALYRLVNWLHVRTRPEVIRRQLLFASGERVSLRVIEETE
                     RLLRGNVYLYDVHIRPVEVRDGVVDVEVSTRDSWSLAPSASLSRAGGTNSGGIGLRDY
                     NVLGSGMRISLASKANVGSTGGVSHTGTDFQQSYPNAFAERVAVGYTRATFAGGSSQG
                     FVLSRPFYALDARWAAGVSLSRDNRPFSIVNSDGSTGQYRRKQEKDEVFGGWSRGLVD
                     GWVQRYSVGLDYERDRYGLDDGVVPAERVPADRKLVAPFFRYEVVQDNFRKVTNVDQI
                     GRPEYFALGFFSSLQYGRALPALGSSEYASLYSGTLGKGFRLPGDATLLASGSFSGEY
                     ANGRGDQQLLGGLLRFYYRQESGSVFYSALSADHARYVDGTLPLTLGGDTGMRGYPSS
                     YQSGNQRVLFTAEERFYTDWYPFRLFRLGSAVFFDHGRAWSEPLSPPNSRWLSDVGFG
                     LRIQSARSATGTTLHVDFAFPVHRDTGIKSYQFSFMSKTGF"
     gene            10779..11144
                     /locus_tag="VI319_04890"
     CDS             10779..11144
                     /locus_tag="VI319_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863215.1"
                     /translation="MRVTRLAVMLALGLSPLASNAEDAVRWRCAYDPAPAPRIACRLV
                     RAPGMDAAAPFPETAGRQLPMLTRIRTVPETLVDERIVIPLWGPPVDMRFVARLATEV
                     MCGRRPDCGVDFSSDPIDE"
     gene            complement(11149..13197)
                     /gene="fusA"
                     /locus_tag="VI319_04895"
     CDS             complement(11149..13197)
                     /gene="fusA"
                     /locus_tag="VI319_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY6863216.1"
                     /translation="MPKYSTEAIRTVALVGHTGAGKTTLAEALLAKAGAIPAPGSVER
                     GNTVCDFDPLEKLYKHSLASSLVNFAWKGTHVHLIDTPGSPDFMGQAVGALAAVETAA
                     VVVNAETGIQLATDRMLKWAAKRGLCRMIVINRIDAENVDLPALLERIQQSVGRECLP
                     INLPAKGATEVVDCFFNPDGESDFSSVKEAHAALVDQVVEVDEALMAKYLEQGEISPE
                     QLHAPFERALREGHLIPVCFVSAKNGAGVESFLDILANLAPNATEGNAPPFLKGEGAD
                     ATEFYAVPGPKAHVLAHVFKIVVDPFVGKVGIFRMHQGTMTPNTQLFIGSGKRPFKVG
                     HLFTIQGKDYVETDALVPGDIGAVTKVDEIEFDCVLHDSHEEDHIHLRPLEFPTPMHG
                     IAVETKKKGDEQRLFEVLHKLEMEDPCFRVERHATTNETVIRGLGELHIRAKLEKMSQ
                     QYKMELATQPPKIAYRETVTRRAEGHCRHKKQTGGAGQFGEVFLRVEPLARGAGFEFV
                     DAVKGGVIPNVFIPAVEKGVRMALDSGVAAGYPVHDLRVTVYDGKHHAVDSKEVAFVS
                     AGRKAAIDAISKAGAIVLEPIVNVEINAPERFMGDLTSDLSGKRGQVTGTDSAGGDLV
                     VVRGLAPLSELADYQTRLKSVTGGQGSYSVDFSHYAPLPGHLQAQLASRHRQPVEEES
                     "
     gene            complement(13300..14460)
                     /gene="clsB"
                     /locus_tag="VI319_04900"
     CDS             complement(13300..14460)
                     /gene="clsB"
                     /locus_tag="VI319_04900"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF008427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEY6863217.1"
                     /translation="MTYELVPGNRITLLRSGVEYFPALEAAIRAARREIYLESYLYEA
                     DETGWRITNALADAARRGVAVHVLVDGFGARGMPPEMVRALQRAGAERLVYRPEISPF
                     RVRKYRLRRMHRKLVAIDGALAFVGGINVIDDIGPDGQGPPRLDFAVRIEGPLLRPIQ
                     QAVQRLWTRVAWVRLHRRRRAKRRVDVDDAPKGDQAAAFVVRDNFRHRSDIEDAYLEA
                     IDGAHDEILIANAYFFPGTRFRRALLRAAARGVRVTLLLQGKIEYTLQHYASRALYGA
                     LLQSGVEIYEYHKSYLHAKVAVIDRRWATVGSSNIDPFSLLLAREANVVVEDRAFAAE
                     LRLALHAALEDGSLVIEGARWSRLSVWRRIPMWVSYGIGRFLMGMVGFGGQV"
     gene            complement(14457..>14987)
                     /locus_tag="VI319_04905"
     CDS             complement(14457..>14987)
                     /locus_tag="VI319_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY6863218.1"
                     /translation="EFAYGRNVVYDHGHHGNAVLSRYPIVNAENEDVSSHEFERRGLL
                     HCEIRVPGLDRHLHCICVHLALNERGRRHQVGALIKRIERECPGDAPVIVAGDFNDWR
                     NLAGKRLAVELGLREAHADQFGRVARSFPCAFPVLRLDRIYVRGFRVNHTEVHRGRPW
                     SRLSDHAPVSAHLQPA"
BASE COUNT         2177 a         5354 c         5219 g         2237 t
ORIGIN      
        1 ccacgtcggc gcccgcgagc atcgcgatcg acaggagaaa gacgatccag cgcttcatgc
       61 cttgagtgta gacccgcggt gcgcggcggg gttctgcttt tacgccttgt tacactgccg
      121 cgtcgcagca aggcctcagc attcgggctc gcccggcgcg ggcgcggggc gcggcacgaa
      181 gccgaagcgc gacgccacgt cggccgagcg tatcggcgcg acgcgcacgc gcgcgccgag
      241 cagcgcgcag tcggcggtct cgccgcgcgc caccggctcg ggcacgcgcg cggccaccgc
      301 cgcgaggtcg cggtccgaca cgacgccgac gccgagcgcg gtctcgagca gcagcgctcc
      361 cgactcgtcg aggaagcccg cgcgcagcgc gccggcgtcg gcgccggtgt gcgacaccag
      421 gcgcatgccc gagtcgtcga gccgccacac ccagggcgtg tacgcgagcg acacgaacac
      481 gcgctgcgga ccgttctgaa aataccagcg cccggcgccg tcggccgcgt aattgcggcc
      541 gatgaaatcg atcaccgccg cgttcgtgat gcggccgaac gcgcccgaga cgcccttgat
      601 cagccacgcg ccgcggcggt cgagcgcgag ccagccgtag accgacggca cgtcgggcca
      661 cctgagcatc gagcggacga cgatttcatc cataatgcga gtctaaatgg accgcggcga
      721 cttcaccttc ttccacacgc tgcgcgtgcg ctgggccgag gtcgaccgcc aggacgtggt
      781 cttcaacggc cactacttcc tctacttcga cgtcgcgatc gcggagtact ggcgcgcgat
      841 cgggttccgc taccccgacg acgtcctcgc gcacggcacc gacatctacg cggtgaaggc
      901 cgccgccgag tatcacgcct cggccaccta cgaagacgtg ctcgacgtgg gctgccgcgt
      961 cgcgcgcatc gggcgctcgt cgatgacgtt cgcgttcggc atctggcgcg gcggcgacag
     1021 gatcacgagc ggcgagctgg tctacgtcaa cgccgatccg aagacgcgca agtcggcgcc
     1081 gtggcccgaa gcgctgcgca gcgcggtgct tcgctacgag cgagcgcgcc ccgacgtcgc
     1141 ttcgaatcgg gactgaggcg ctcagcgcgg ctgcgcgagc cgctcgagca tcgcctgcac
     1201 ggcgcgcacc tgcgcgccct ccttcgccgc gtagcgcgcg ccggtgtacc cccaccagtc
     1261 ccagcaggcc ttcggattga atacgaaatt gccggacgcg aagctgtagc cccagcgcgg
     1321 cgtcgtctgc gggtagagca cgatcagccg gttcgccgcc gcccagcggt tgtacccggc
     1381 ctcgagcacg aaacgcttgt cgatctgggt cgcgctctgg cggcatccgt ggaagacgac
     1441 gtggacgcgg caacgctcct tcgcgcacgc gtccgggacg aagacgtagc ccgcatcgcc
     1501 catgctgatc gccgccggat ttccgccggc gaactcgcgc tgatcgaact cgagaagctt
     1561 gccgtcctgt ttcggtgtcg gcgcggcgag cggcccgagc agttgagcga gcaggatgcc
     1621 cgcggcgtcg taggcgcagt cgacgatgaa cggcggctgc gtggtcgagc actcgttgcc
     1681 ctcttcctgc gtcggcatgc cgtggccggc gggcacgctg ttctcgaacg cgacgtcggc
     1741 gtcgggcgcg agcgcgaggt agaagcgccg cagcgcctcg accacctcgg aggagacggt
     1801 gtcgtcgcga gtccccgtga acagccacag cttcgcgccg gcgagccgct cgaccgggtc
     1861 gatcccgccg gccttggcga ggccgcgcgc gagctcggcg aggcgccgcg cgtctgggag
     1921 cggcgagaac gggccggggt ccatgcagct cgtgagcgcg gtccagaggc tcccctgcgc
     1981 gcaatcgtag ggcccgcccg cgatcgcgcc cgcgccgcgc acggtcgccg aatgcgcgac
     2041 gtggaactgg accgccatga acgcacccga ggacacgccc gagacggtga ccgccgcgcc
     2101 gtcggccccg agcgcgggca gccgctcggc ggccctcgcg ggcgacgcga gcaggaggac
     2161 gagcgcaaga agaagacgag cgagcatgcc gggcacgccg tattgggcat ttccttataa
     2221 tcgccgttca ctcccgtgtc caatcttccc atgcagaacc gcgaccagct ctacgtcgac
     2281 gggcgctgga ccgcctccgc cggcgcaaac accatcgccg tcgtctcgcc gtccaccgag
     2341 gagcctatcg gccgcgtgcc cgaggggaca ccggacgatg tcgattcggc ggcgaccgcc
     2401 gcccgcgcgg ccttcgattc ctgggcggcg acgccgcccg ccgggcgcgc cgccttcctc
     2461 tcgaagatcc acgagggcct gaaagcgcgc gccgacgaga tcgggcgcac gatcgccgcc
     2521 gaggtcggca tgccgctcaa gctcgcgacg cgcatccagg cgggctcgcc gatctacacc
     2581 ttcgcgatgt acgcgaagat gctcgggagc tttgcgttcg aagaacgcgt cggcaactcg
     2641 atcgtgctgc gcgagccggt cggggtcgtg ggcgcgatca cgccgtggaa ctatccgctg
     2701 caccagatcg ccgccaaggt ggcgccggcg ctcgccgccg gctgcaccgt ggtgctcaag
     2761 ccctccgagc tcgcgccgct caacgccttc atcctcgccg aggtcgtgca tgccgcggga
     2821 ctgccgaagg gcgtgttcaa tctcgtcacc ggctacggcc cggtcgtggg cgaggcgatc
     2881 gcgcgccatc ccggcgtgga catggtttcc ttcaccggct cggtgcgcgc cggcaagcgc
     2941 gtcgccgagc tcggcgcgca gcaggtcaag cgcgtgacgc tcgagctggg cggcaagtcg
     3001 gcggcgatcg tactcgacga cgccgacctc gcgcaggccg tgaagagcac cgtcaacgcg
     3061 tgcttcctca acaccggcca gacctgcagc gcgcacacgc ggctgctcgt gcccgagagc
     3121 cgctacgagg aggccgcgcg cctcgcggtc gaggcggcgc agggcttcac cgtcggcgat
     3181 cccttcggcg acgcgaagct cggcccggtc acgtcgaagg cgcagctcga gcgcgtgcgc
     3241 ggctacatcg cgaaaggcgt cgccgaaggc gcgcagctgc tcaccggcgg cgccgagcgg
     3301 cccgatggcc tcgcgcgcgg ctggtacgtc cggccgaccg tgttcgggcg cgtcgacccg
     3361 cactcgacgc tcgcgcagga agagatcttc ggcccggtcc tcgcgatcat cacctaccgc
     3421 gacgacgacg aggcggtgcg catcgccaac gataccatct acggcctcgc cggcggcgtg
     3481 tggtcgaagg acgaggagcg cgccaagcgc gtcgcgcggc ggctgcgcac cgggcaggtc
     3541 gacatcaacg gcggcgcctt caacatgcag gcgccgttcg gcggctacaa gcagtcgggg
     3601 cgcgggcgcg agctcggccg gcccggcctc gaggagtatc tcgagtacaa gtcgctgcag
     3661 ctgaaggccg aactcaggtc cgaatcccgg tcctgagccg gtcccgagcc ggtgatcctc
     3721 tcgcacaagg tccacgtcct cacccgcaaa gaggagctcg acaccgtccg ccttcccggc
     3781 aaggtggtga tcgtgctcga cgtgctgttc gcgacctcga ccatcgtcgc ggcgctggcg
     3841 gcgggcgcga ccgaggtgat cccgacgctc gacggcgccg cggcgcgcgc cgtggccgcg
     3901 cgcgaggacg cgggcggctt cgtcctcgcc ggcgagctga acacggtgac gctcgagggg
     3961 ttcgcgccgc cgacgccgct cgcgctgatc gagcacggcg tcgcgggccg caagctcatc
     4021 tactcgacga ccaacggcac cgtcgcgttc cacgcgtgct cgggcgcgca ggatgtctac
     4081 gcgggggcgc tgctgaacgg cgaggcggtg gtcgaccggg tcgtctccgc gcacccggac
     4141 cgaacgctgc tgatcgtgtg ctcgggctcg gccggcaatt tcaacctcga ggacttctac
     4201 ggcgcgggct acctcgtcga gctgctcgcg agccggctcg gcgacggcgc cgacttctcc
     4261 gatgcggcgc gcgccgcgcg cgcgctctac cgctcgggcg cgccggagga gacgctgctc
     4321 gcctgccgcg tggggcgcat gatgatcgtg cgcggcctcg accgcgagat ccgctacgcc
     4381 gcgcagcgct cggtcgcgcg cgtggtgccg cgctacgacg gcgcggcgct gcgaccgtgc
     4441 tagcattcgg gcgcagggac gctgtgcaag ggggagacat gaagctcatt ttcgcggcgc
     4501 tggtcgcgct cgccgcggcg ggcggggtgt ccgcgcagga atcggccgcc gactacccga
     4561 gccgcgccgt gcgcctggtc gtgccgaacg cgccgggcag ctcgatcgac accatgagcc
     4621 gcatcgtcgc ggcgaagctc ggagagacgc tgggccagtc cgtgttcgtc gagaaccgcg
     4681 acggcgccgg cgggctgatc ggcgtcgagg cggcgaggag cgcgaagccc gacggctaca
     4741 cgctgctgtg cgcgtcgaac ggctcgatga tgatcgcgcc gctgctgaag aagccggttc
     4801 cgtacgacgc gctcaatgac ttcacgctga tcggtccgtt cgccgtcacc ccgaacgtgc
     4861 tggtcgtcaa tcccgagctc ccggtgaaga ccgtgaagga gctgatcgag tacgccagga
     4921 cgcacgcctc gaccatcaac atggcttcgg cgggcgtggg ctcgcagagc catctctcgg
     4981 gcgtgctgct catgacgatg gccggcttcg actcgctcca cgtcccgcac aagggcggcg
     5041 gaccgtcggt gaactcggtg gtcgcgggcc agacgcactg gaccctgacg ccggcgcccg
     5101 ccgcgatatc gttcgtgcgc aacggcaagc tgcgcgcgct cggccagagc ctgccgcacc
     5161 gctcgtcgat tctgggcgac atgccggcga tctcggagac ggtgcccggc tacgactaca
     5221 gcggctgggc cgggctggtc gcgccgaagg gcgtgcccgc cccggtcgtc gacaagctgc
     5281 gcgacgcgct gacgaagacg ctgtcgcttg ccgacgtcaa ggagggcctc gcgcgccagg
     5341 gcgccgagga gctcaccggc acgcccgacg agttccgccg cttcatcgag cgcgaccacg
     5401 ccaacacggt gaaggtcatc cgcgcggcga agctgcagtc cgagtagggg cggcgtggac
     5461 ctgctgccgc cctcggcgct gctcgaatcg ccgctcgcga tcgcggggct cgcgctgggc
     5521 gcggccggcg cgatcctcgt cctcgccggc atgatcgcgc tcgtgcgcat gcggccggtg
     5581 cgcttcgcgc tgcgcacgct cgccggcctg ctgctgctgt cgctcgcggg gctcgcgggc
     5641 gcggtgctgc tcggcgtgca aggctaccgc gccttgaccc gggaggacgt ggcggcccgg
     5701 gtctcggtgc ggccaaccgg gccgcagagc ttcgccgcga ccgtgcgcac ggccgacggg
     5761 cgccaggcgt cgtacgccct cgccggcgac gagatctaca tcgacgcccg catcctcaag
     5821 tggaagccgg tcgccaatgt gctcggcctg cacaccgagt acgagctcga ccgggtcgcg
     5881 ggccgctacc gcgacgtcga gcaggagcgc agcgcgccgc gcagcgtcta cgcgctcggc
     5941 accgagaagc cggtcgacct gttctcgctg cggcggcgct acgccttcct cgcgccgctg
     6001 ctcgacgccg aatacggatc ggcgaccttc gtgccggtga cggagcccgc cgagctcgag
     6061 ctgcgcgtct cgaccacggg gctcctgatc cgcgacgcca cgccgccgcg gccctgaagg
     6121 ccggcgccgt caatccgccc ggatgctcgc ggcgcgcgcg atgcggctga acaccgcctc
     6181 gtcgtggcgg atgagcgcgt cgaaggcctc gggcgccatc ggacgcggcg tcatcccgat
     6241 cggcgcccag cgggcccgcg tctccggatc ctggaggacg cggacgatgt cgcggttgag
     6301 cctggcgacg atcggcctcg gcaccgccga ggacgtgagc aggccgaacc agaggatcga
     6361 ctcgtagccc gggacgcccg cctcggagag cgtcggcaga tccggcagca gcggatcgcg
     6421 ctcgcgcgag gacaccgcga gccccacgag cttgccgtcg cgcacgaggt tgaccgagtt
     6481 ggcgatgggc gccatgaaga actgaacgcg ccccgtggtc gtctcggtga gggcctcggg
     6541 aatgcctttg aagggaacgt gcacgacgtc gatgccggcc atcgccttca gcatctcgcc
     6601 ggcgaagtgc gttccgctgc cgaccccggc cgaggaaaag ttgagctggc cgggcgccgc
     6661 tttcgcgcgc gcgatcagct ccttcaccga acgcagcccg agcgagggcg cgacgaccag
     6721 cacgtagctc gagctggccg tctgcgtgat cccggcgagg tccttgaagg tgtcgtacgg
     6781 cagcgtccgg tagatcgccg gcgccacggc gtgcgccgac gagaccgaca gcagcgtgta
     6841 gccgtccggc gccgccttcg ccacggcgtt ggccgcgatg gtgccgcccg cgccgggccg
     6901 gttgtcgacg aggacctgct gcttccacgt ctcgccgagc ttctgcgcga tgtagcgcgc
     6961 ggtgatgtcg ggaccgccgc cgggcgtgaa ccccaccatg atatgtaccg gccgcgcggg
     7021 ataatcgcct gccgcggcgc cgcccctgga ctgctgcgcc atcgcgcagc acgacgccgc
     7081 cgcgagcgcc agcgcggccg cgatcgatgc tctcgtctgc ttcattccgc cttcctccgt
     7141 aaggatggcg gcgattctat ccggacgcgc gatggcgtac catggcggcc cgagacccgc
     7201 tcgcagcgcc acaaggccac acgatgtcgc cggaaatcga cgccgccgcg cgacggccgc
     7261 gcggccccgt ccgccgcgtg catgaactcg aaccctgcgc gagagtctct acgctcacta
     7321 tgcccacgct tcgccggcgc gtttgactct gcacatcgca gggctcgcgc gttttctcgc
     7381 ggttctcgcg gtgtgcggcg cgggcctcgc ggcggcgcag gatcccgagg agaacgtccc
     7441 gccggtgccg accttcgccg agctcgaggc cgccggcgcc gtgatcggtg agatccacgt
     7501 cgacagcgag gacatcttcg atctcaccga tccgcgcgag gacaagccgc tctacagcct
     7561 ggcgaactgg ctccacatcc gcacgcgccc ggacgtgatc cgcaggcaat tgctgttcgc
     7621 gagcggcgag cgcgtctcgg cgcgcctgat cgacgagacc gagcgcaacc tccgcggcaa
     7681 cgactatttc tacgatgtcg ccatccggcc gctggacgtc cacgacggcg tcgtcgacat
     7741 ccaggtgaag accagggaca cctggacgct cgtgccgacg gccaacctca gccgctccgg
     7801 cggcgtgaac cgtggcggcc tcggattccg cgattccaac ttcctcggca cgggcacgag
     7861 gttcagcatc ggccgcaagg cgagctccga caacaccggc aacaccgcgc ccgcgagcct
     7921 cgacctgcag ctgtcgcaag cgaacgcgtt cgacggccac acggccttga gctacaacct
     7981 gtcgcgcttc gccgacgggc gccacgacgg cctgtcggtc gctcggccgt tctacgcgct
     8041 cgatacccgc tggtcggccg gcgcctccgg agcgcgcgac gaccgcacca tcttctcgtt
     8101 cgtgggcgcg aacaccgcgg tgcccatcta ccggcggcgc gaggacaccg cgtcggtcgc
     8161 cggcggctgg tcgccgggac tcgcggacgg atggacgcac cgctattcgc tcggcctctc
     8221 gtggctgtcg gacaactaca gccctgccgc cgacgcggcg ccggacgcgc agatcccgac
     8281 cgaccggacc ctcgtgtctc cgttcctgca gtacgaggcg gtccaggacg atttccgcaa
     8341 ggtcgtcaac cgcgacctga tcgagcgcac cgagatcttc gccatgggcc tgcatgcgaa
     8401 cgcgcagttc gggcgctcgc tgccccagct ggggtcgacg cagtacgtct cgatgtactc
     8461 gggcggcctg agcaagggct tcgatgtcgg atcgagcggc acactgctcg cctcggggtc
     8521 ggtctcgggc gagtacggcg accatcacaa cgaccgccag gtgggaaaca cgaccttcaa
     8581 gtactacctc gcgcagagcg cgagcgcggt gttcttcgcc tccttcgccg ccgaccggct
     8641 gcggttcaag gacggctcgc agcagctcct gctcggcgga gacaacggcc tgcgcggcta
     8701 tcccgcgcac taccagagcg gcgaccggcg cgtgctcttc accgcggagc agcgcctcta
     8761 caccgactgg ttcccgtacc ggctgttccg cgtggggggt gcgatcttcc tcgacgccgg
     8821 gcgcgcctgg agtgggccgc tcgacgagga gccggcgaac cggcgctggc tcgccgacgt
     8881 cggcttcggg atccgcgtcc tgaacgcgcg ctcctcgagc gggacgaccg tgcacatcga
     8941 tttcgccttc ccgctgcacc gcgaggacgg catcaagtcg tatcagttct cgttcatgtc
     9001 gaagacggga ttttgatcgg cgtcagcccg gcgcaggacg ggcgcgctat cttgtcccgg
     9061 tgcgctcttc ctgcaatccg tacccgtgag ccggccgctc gccggctggg cgctcctgct
     9121 cgcgtgcatc gccgctcccg catggagcca ggctgatccc gacgccgctc gcctgccctc
     9181 cttcggcgag ctcgaggccg ccggcgccgt gatcggcgag atccgcatcg acaaccagaa
     9241 cgtcttcgac gagcgcgacg cgcgcgagaa caacgccctg taccggctcg tgaactggct
     9301 gcacgtgcgc acccggcccg aggtgatccg caggcagctc ctgttcgcga gcggcgagcg
     9361 ggtttcgctg cgcgtgatcg aagagaccga gcggctgctg cgcggcaacg tctacctgta
     9421 cgacgttcac atccgccccg tcgaggtccg cgacggcgtc gtggacgtcg aggtgagcac
     9481 gcgcgacagc tggtcgctcg cgccgagcgc gagcctcagc cgcgccggcg gcaccaacag
     9541 cggcggcatc ggcctgcgcg actacaacgt gctcggctcc ggcatgcgca tcagcctcgc
     9601 gtccaaggcc aacgtcggca gcaccggcgg cgtgagccat accggcacgg atttccagca
     9661 gtcgtatccc aacgcgttcg ccgaacgcgt cgcggtcggc tacacgcgcg cgacgttcgc
     9721 cggcggcagc agccagggat tcgtgctgag ccggccgttc tacgcgctcg acgcccggtg
     9781 ggcggcgggc gtctccctct cgcgggacaa ccggccgttc tcgatcgtga acagcgacgg
     9841 cagcaccggc cagtaccggc gcaagcagga aaaggacgag gtgttcggag gctggtcgcg
     9901 cggcctggtg gacggctggg tccagcgcta ttccgtcggg ctcgactacg agcgcgaccg
     9961 ctacggtctc gacgacggcg tcgtccccgc ggagcgcgtt ccggccgatc gcaagctggt
    10021 ggcgcccttc tttcgctacg aggtggtgca ggacaatttc aggaaagtga ccaacgtcga
    10081 ccagatcggg cgccccgagt acttcgcgct gggattcttc tccagcctgc agtacggccg
    10141 cgcgctgccc gcgctcgggt cgtcggaata cgcctcgctc tattcgggca cgctgggcaa
    10201 gggcttccgt ctcccgggcg acgccacgct gctcgcttcc gggtcgttct ccggggaata
    10261 tgcgaacggc cgcggcgacc agcagctgct cggcggcctg ctgcgcttct attaccggca
    10321 ggagagcggc tcggtcttct attccgcgct gagcgccgac cacgcccggt acgtggacgg
    10381 cacgctgccg ctcacgctcg gcggggacac gggcatgcgc ggctatccct cgagctatca
    10441 gagcggcaac cagcgcgtgc tgttcacggc cgaggagcgc ttctacaccg actggtaccc
    10501 gttccggctc ttccggctgg gctcggccgt cttcttcgac catggacgcg cctggagcga
    10561 gcccctgtcg ccgcccaact cgcgctggct gagcgacgtc gggttcggct tgcgcatcca
    10621 gagcgcgcgc tcggcgaccg ggacgacgct gcacgtcgac ttcgcgttcc ccgtgcaccg
    10681 cgacaccggg atcaagtcgt atcagttctc gttcatgtcg aagaccgggt tttgacccca
    10741 gttgatacaa tgaaatatcg atcctgggag ggatcctgat gcgcgtgacg cgcctcgccg
    10801 tgatgctggc gctcgggctg tcgcccctcg cgtccaacgc ggaggacgcg gtgcgctggc
    10861 gctgcgcgta cgatcccgct ccggctccgc gcatcgcgtg ccgcctcgtg cgagcgccgg
    10921 gcatggatgc ggcggccccg tttcccgaga ccgcgggccg ccagctgccg atgctgaccc
    10981 gtatccgcac ggtccccgag acgctcgtcg acgagcgcat cgtcatcccg ctgtggggcc
    11041 cgccggtcga catgcgcttc gtcgcgcggc tcgcgacgga ggtcatgtgc ggaagacgcc
    11101 cggactgtgg ggtggacttc tcttccgatc cgatcgacga gtgaggcgct agctttcctc
    11161 ctcgaccggc tgccgatgcc tggacgcgag ctgcgcctgc agatgcccgg gcagcggcgc
    11221 gtagtggctg aagtcgaccg agtacgagcc ctgtccgccc gtcaccgact tgaggcgggt
    11281 ctggtagtcg gcgagctcgg aaagcggcgc gaggccgcgc acgacgacga ggtcgccgcc
    11341 tgcggagtcc gtgccggtca cctgcccgcg cttgcccgag aggtccgacg tgagatcgcc
    11401 catgaagcgc tcgggcgcgt tgatctcgac gttcacgatc ggctccagca cgatcgcgcc
    11461 ggccttggag atcgcgtcga tggccgcctt gcggccggcc gacacgaagg cgacctcctt
    11521 ggaatccacc gcgtgatgct tgccgtcgta gacggtcacg cgcaggtcgt gcaccgggta
    11581 gcccgccgcg acgcccgagt cgagcgccat gcgcacgccc ttctcgaccg cggggatgaa
    11641 cacgttcggg atcacgccgc ccttcaccgc gtcgacgaac tcgaacccgg cgccgcgcgc
    11701 gagcggctcg acgcgcagga acacctcgcc gaactgcccg gcgccgccgg tctgcttctt
    11761 gtgccggcaa tggccttcgg cgcggcgcgt gaccgtctcg cggtacgcga tcttcggcgg
    11821 ctgcgtcgcg agctccatct tgtactgctg gctcatcttc tcgagcttgg cgcggatgtg
    11881 cagctcgccc aggccgcgga tcacggtttc gttggtcgtc gcgtggcgct cgacgcggaa
    11941 gcaaggatcc tccatctcga gcttgtggag cacctcgaac agccgctgct cgtcgccttt
    12001 cttcttggtc tcgaccgcga tgccgtgcat gggcgttgga aactcgagcg gccgcaggtg
    12061 aatgtggtcc tcctcgtgcg agtcgtgcag cacgcagtcg aactcgatct cgtccacctt
    12121 ggtcaccgcg ccgatgtcgc ccggcacgag cgcgtcggtt tcgacgtagt ccttgccctg
    12181 gatcgtgaac aggtgcccga ccttgaaggg gcgcttgccg ctgccgatga agagctgcgt
    12241 attcggagtc atcgtgccct ggtgcatgcg gaagatcccg accttgccga cgaagggatc
    12301 gaccacgatc ttgaacacgt gcgcgagcac gtgcgccttc gggcccggca cggcgtagaa
    12361 ctcggtcgcg tccgcgccct cgcccttcag gaacggcggc gcgttgccct cggtcgcgtt
    12421 gggcgcgagg ttcgccagga tgtcgaggaa ggactcgacg ccggcgccgt tcttcgccga
    12481 cacgaagcac accgggatga ggtgcccttc gcgcagcgcg cgctcgaacg gcgcgtgcag
    12541 ctgctcgggg ctgatctcgc cctgctcgag gtacttcgcc atcagcgcct cgtccacttc
    12601 cacgacctgg tcgacgagcg ccgcgtgcgc ctccttcacc gacgagaaat cggactcgcc
    12661 gtcggggttg aagaagcagt ccaccacttc ggtcgcgccc ttcgccggca ggttgatcgg
    12721 gaggcactcg cggccgaccg actgctggat gcgctcgagc agcgccggga ggtcgacgtt
    12781 ctccgcgtcg atgcggttga tcacgatcat ccggcacagg ccgcgcttcg ccgcccattt
    12841 gagcatgcgg tcggtcgcga gctggatgcc ggtctcggcg ttcaccacca cggccgcggt
    12901 ctcgaccgcg gcgagcgcgc cgaccgcctg gcccatgaag tcgggcgacc cgggcgtgtc
    12961 gatgaggtgc acgtgcgtgc ccttccacgc gaagttgacg agcgacgagg cgagcgaatg
    13021 cttgtagagc ttctcgagtg gatcgaagtc gcagacggtg ttgccgcgct cgacgctgcc
    13081 cggggccggg atggcccccg ccttcgcgag cagcgcctcg gcgagggtgg tcttgccggc
    13141 gccggtgtgg ccgacgagcg cgacggtgcg gatggcttcg gtcgaatact tcggcatggg
    13201 ccggggctcc tcgctgagat ggccggacgt cgggaacgag ggtgcgttcc ccatttaact
    13261 cccagcccgg gcggtcccgc aagcgcccgg gagacggcct cagacctggc cgccgaaccc
    13321 caccatgccc atgaggaacc ggccgatgcc gtacgacacc cacatcggga tgcgccgcca
    13381 gacggacagc ctggaccagc gggcgccttc gatcacgagc gagccgtcct cgagcgccgc
    13441 gtgcagcgcg aggcgcagct cggccgcgaa ggcgcggtcc tcgaccacga cgttcgcctc
    13501 gcgggcgagc agcaggctga acggatcgat gttcgacgag cccacggtcg cccagcgccg
    13561 gtcgatcacg gcgaccttcg cgtgcaggta gctcttgtgg tactcgtaga tctccacgcc
    13621 ggactggagc agcgcgccgt agagcgcgcg cgaggcgtag tgctggagcg tgtactcgat
    13681 cttcccctgc agcaggagcg tcacgcgcac gccgcgcgcc gccgcgcgca gcagcgcccg
    13741 gcggaaccgg gtcccgggaa agaagtacgc gttcgcgatg aggatctcgt cgtgcgcgcc
    13801 gtcgatcgcc tcgaggtacg cgtcctcgat gtcgctgcgg tgtcggaagt tgtcgcggac
    13861 gacgaaggcg gcggcctggt cgcctttcgg cgcgtcgtcc acgtcgacgc ggcgcttcgc
    13921 gcgccggcgc cggtgcagcc gcacccaggc gacgcgcgtc cacagccgct gcaccgcctg
    13981 ctggatcggg cgcagcagcg gcccctcgat ccgtaccgcg aagtccaggc gcggcggccc
    14041 ctggccgtcg ggcccgatgt cgtcgatgac gttgatgccg ccgacgaagg cgagggcccc
    14101 gtcgatcgcg accagcttgc ggtgcatccg acgcagccgg tacttgcgga cgcggaacgg
    14161 cgagatctcg ggccggtaca ccaggcgctc ggcgccggcc cgctgcaatg cccgcaccat
    14221 ctcgggcggc atgcccctcg cgccgaagcc gtcgacgagg acgtgcacgg cgacgccgcg
    14281 gcgcgccgcg tcggcgagcg cgttggtgat gcgccagccg gtctcgtcgg cctcgtagag
    14341 ataggactcg aggtagatct cgcgccgggc cgcgcggatc gccgcctcga gcgcgggaaa
    14401 gtactcgacc cccgagcgca acagcgtgat gcggttgccg gggacgagct cgtaggtcat
    14461 gccggctgca ggtgggccga tacgggtgcg tgatccgaga gccgcgacca gggccgcccg
    14521 cggtgcacct cggtatggtt gacgcggaag ccgcgcacgt agatgcggtc gaggcgcagc
    14581 accggaaacg cgcacgggaa gctgcgcgcg acgcggccga actggtccgc gtgcgcttcg
    14641 cgcaacccca gctcgaccgc aaggcgcttg ccggcgaggt tgcgccagtc gttgaagtcg
    14701 cccgcaacga tcaccggcgc gtcgcccggg cactcgcgct cgatgcgctt gatcagcgcg
    14761 ccgacctggt gcctgcggcc gcgctcgttc agcgcgagat gcacgcagat gcagtgcagg
    14821 tgccggtcca gcccgggcac cctgatctcg cagtggagca ggccgcggcg ctcgaactcg
    14881 tgcgacgaga cgtcctcgtt ctcggcgttg acgatgggat agcgcgacag caccgcgttg
    14941 ccgtggtggc cgtggtcgta gacgacgttg cggccgtacg cgaactc
//
LOCUS       DATDMW010000109         2871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2271918, whole genome shotgun sequence.
ACCESSION   DATDMW010000109 DATDMW010000000
VERSION     DATDMW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271918"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1511
                     /locus_tag="VI319_04910"
     CDS             210..1511
                     /locus_tag="VI319_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY6863219.1"
                     /translation="MDLAVVCLLILLNGFFAMSEMALVSARKARLQRWAEEGRPGAAP
                     ALDLAERPVHFLSTVQVGITSIAILTGALGEDAIANRLGAWLAGFAPIAPYAHGLALA
                     ATVLVITYFSVVVGELVPKQLALRSPEAIASRIARPMQLLARAAAPLVRLLGASSAWI
                     LRAAGAGPREGAPVTDEEIVVLMRQGARAGVFEKAEPALVSNILHLDEQPVGAIMTPR
                     LDMLYLDLYDDFAVNRRKIVASPDWIVPVCRGGSDEVVGMLHTHDLLGRCLAGDEVDL
                     AATLRAPFIIPPTLSVLQVLEGFRTHRVEVALVADEYGHPLGLVSLKHVLEAIVGELP
                     AHGEDDTGAARRDDGSWLLDGAIDLHRFRQLLDVGDLPGEEEGDFHTLGGFVMAQLGR
                     VPRAGDAFRWKDLRVEVLDMDRNRVDKVLVSRVERAETEPS"
     gene            complement(1515..2693)
                     /locus_tag="VI319_04915"
     CDS             complement(1515..2693)
                     /locus_tag="VI319_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY6863220.1"
                     /translation="MRPLTGITVVTLEHAIAAPFATRQLADLGARVIKVERPGVGDFA
                     RGYDERVRGLSSHFVWTNRGKESLTLDVKQPEALALAKRLVLERADVLVQNLAPGAAA
                     RLGLDHASLAPDKPGLIVCDISGYGADGPYRDKKAYDLLIQSEAGVLSVTGTPEEPVK
                     TGISVADIAAGMYAYSNILAALIERARTGRGRRIDVSMLESLVEWMSYPLYYAFDGAA
                     PPERTGARHATIYPYGPFPARDGATVMLGIQNEREWAAFCDKVLARPELASDPRFSSV
                     SKRAAAREQLAPIIAAVFSRLTGDDLVRRLDAAQIANARVNDMRGVWAHPQLRARERW
                     RDVDTPAGPVPAPAPPGIPSEDARMDPVPALGEHTGPILRELGLGDADVARLRAAKAI
                     "
     gene            complement(2707..>2871)
                     /locus_tag="VI319_04920"
     CDS             complement(2707..>2871)
                     /locus_tag="VI319_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY6863221.1"
                     /translation="QAVVRRYEFRAVRPLFDINPFEVCGEPSADGKSVRLWARDHEGW
                     LAMDATAQIG"
BASE COUNT          380 a         1016 c         1043 g          432 t
ORIGIN      
        1 ttcccacggt cattgttcgg attccttccg ttgttcctct cgcacggcaa gcgtagggcc
       61 ggcgtgtgtc gcgaacggcg cggtcccgaa cgcgcgcact gggcaggttg aaccactcgg
      121 ccgcgtccga gcaccgcggg gcgcgaccga ggtccaagcg acagtgcgac cgcgcgccgc
      181 gcggcccggc cgcatgcttc cttcggctca tggatctcgc cgtcgtctgc ctgctcatcc
      241 tgctgaacgg cttcttcgcg atgtcggaga tggcgctcgt ctcggcacgc aaggccaggc
      301 tgcagcgctg ggccgaggaa gggcgaccgg gcgccgcgcc ggcgctcgat ctcgccgagc
      361 ggccggtgca tttcctttcc accgtgcagg tcggcatcac cagcatcgcg atcctcaccg
      421 gcgcgttagg cgaggacgcg atcgcgaacc ggctcggcgc gtggctcgcc ggcttcgcgc
      481 cgatcgcgcc gtacgcgcac ggcctcgcgc tcgcggcgac ggtgctggtc atcacctatt
      541 tctcggtggt cgtgggcgag ctggtgccga agcagctcgc gctgcgcagc cccgaggcga
      601 tcgcctcgcg catcgcgcgg ccgatgcagc tgctcgcgcg cgcggcggcg ccgctcgtgc
      661 gcctgctcgg cgcgtcgagc gcctggatac tgcgcgccgc tggcgccggg ccgcgcgaag
      721 gcgcgcccgt gaccgacgag gagatcgtgg tgctgatgcg ccagggcgcg cgcgcgggcg
      781 tgttcgagaa ggcagagccg gcgctggtct cgaacatcct gcacctggac gagcagccgg
      841 tcggcgcgat catgacgccg cgcctcgaca tgctctatct cgacctctac gacgacttcg
      901 cggtgaatcg ccgcaaaatc gtcgcgagcc ccgactggat cgttcccgtg tgccgcggcg
      961 ggagcgacga ggtggtcggc atgctgcaca cgcacgatct gctcggccgc tgcctcgccg
     1021 gggacgaggt cgacctcgcg gcgacgctgc gcgcgccctt catcattccg ccgacgctgt
     1081 cggtgctcca ggtgctcgag ggcttcagga cgcatcgcgt cgaggtcgcc ctggtcgccg
     1141 acgaatatgg ccaccctctc ggcctcgtct ccctcaagca cgtcctcgag gcgatcgtcg
     1201 gcgagctgcc cgcgcacggc gaggacgaca cgggggcggc gcggcgcgac gacggctcgt
     1261 ggctcctcga cggggcgatc gatctgcacc gcttccggca gctgctcgac gtcggcgacc
     1321 tgccgggcga ggaggagggc gacttccaca cgctcggcgg cttcgtgatg gcgcagctcg
     1381 gccgcgtgcc gcgcgccggc gacgcgttcc gctggaagga cctgcgcgtc gaggtcctcg
     1441 acatggaccg caaccgcgtc gacaaggtgc tggtgtcgcg cgtcgagcgc gcggagaccg
     1501 agccctcctg aacgttagat cgccttcgcg gcgcgcagcc gcgcgacgtc ggcgtcgccc
     1561 agccccagct cgcgcaggat cggcccggtg tgctcgccga gcgcgggcac cgggtccatg
     1621 cgcgcgtctt ccgacgggat cccgggcggc gcgggcgcgg ggacggggcc cgcgggcgtg
     1681 tcgacgtcgc gccagcgctc gcgcgcgcgc agttgcggat gcgcccagac gccgcgcatg
     1741 tcgttcacgc gcgcgttcgc gatctgcgcg gcatcgaggc gccgcaccag gtcgtcgccg
     1801 gtcaggcgcg agaacaccgc ggcgatgatc ggcgcgagct gctcgcgcgc ggcggcgcgc
     1861 ttcgagacgc tcgagaagcg cggatcggac gcgagctcgg ggcgcgcgag caccttgtcg
     1921 cagaacgccg cccattcccg ctcgttctgg atgccgagca tgacggtcgc gccgtcgcgc
     1981 gcggggaacg ggccgtaggg atagatggtc gcgtggcgcg cgccggtgcg ctcgggcggg
     2041 gcggcgccgt cgaaggcgta gtagagggga tagctcatcc actcgaccag cgactcgagc
     2101 atggagacgt cgatgcgccg gccgcgtccg gtgcgcgcgc gctcgatgag cgcggcgagg
     2161 atgttcgagt aggcgtacat gcccgccgcg atgtcggcga ccgagatgcc ggtcttcacc
     2221 ggctcctcgg gcgtgccggt caccgacagg acgcccgcct cgctctggat cagcaggtcg
     2281 tacgccttct tgtcgcggta cgggccgtcg gcgccgtagc cggagatgtc gcagacgatc
     2341 agcccgggct tgtccggcgc caggctcgcg tggtcgagcc cgaggcgcgc ggccgcgccc
     2401 ggcgcgaggt tctggaccag cacgtcggcg cgttcgagca cgaggcgctt cgcgagcgcg
     2461 agcgcctcgg gctgcttcac gtccagcgtg aggctctcct tgccgcggtt ggtccagacg
     2521 aagtgcgacg acagcccgcg cacgcgctcg tcgtagccgc gcgcgaagtc gccgacgccg
     2581 gggcgctcga ccttgatgac gcgcgcgccc agatcggcga gctgccgcgt cgcgaacggc
     2641 gcggcgatgg cgtgttcgag tgtgacaaca gtgataccgg taagcggtct catcgaatgt
     2701 gctccatcaa ccgatctgtg cagtggcgtc catcgctaac cagccttcgt ggtccctggc
     2761 ccacaagcgc acgctcttgc catccgccga cggctcgcca catacctcga aaggattgat
     2821 gtcgaataac ggccgcacgg cgcgaaactc gtagcgccgc accacggcct g
//
LOCUS       DATDMW010000110         9755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_280011, whole genome shotgun sequence.
ACCESSION   DATDMW010000110 DATDMW010000000
VERSION     DATDMW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280011"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VI319_04925"
     CDS             <1..581
                     /locus_tag="VI319_04925"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY6863222.1"
                     /translation="RVGPGKRPRSSMAPTLVFDAEGDAVSVTASIESAFGNRQMVRGF
                     MLNNELTDFSWLPEADGKPVANRVGPGKRPRSSMAPTLVFDAEGRLLLAIGSPGGHSI
                     INYVAKTLVGVLDWGLYVQDAISLPNFGSRNGPTDLERGTPLERLVPQLRAMQHVVRI
                     RPEPSGLHGIQRVPGGWLGGADPRREGAAVGE"
     gene            671..1336
                     /locus_tag="VI319_04930"
     CDS             671..1336
                     /locus_tag="VI319_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863223.1"
                     /translation="MKIGKIVSVIAVLAAAGAAHAEEGIGLRVGTLGYGGDFGWHIAP
                     GLNARLGYSTYSHNTSITNTDVHYDGKLKLSNAPLLFDFSPIGPFHFTAGIIGNANKI
                     DITGTPASNGTFTFNGQTFTSSQVGSVSGRLKLGNSVAPYLGIGWGNVAGAGVNFYAD
                     LGVAYMGSPKASLSATCGSGVPASTCAQIQADVEAEQRALEDKVKKYKYYPVLGIGLT
                     IGF"
     gene            complement(1400..1906)
                     /locus_tag="VI319_04935"
     CDS             complement(1400..1906)
                     /locus_tag="VI319_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY6863224.1"
                     /translation="MAIKPGDKLPDGKLSESTEFDEAAGCPMPPKDISVADSVKGKKI
                     VIFALPGAYTPTCSAKHVPGYVKNYDKLKSKGVDEIWCVATNDGFVMAAWGRDQKAGG
                     KIRMLGDGSAEWTKKLGLELDLSARNMGLRSQRYSMLVDNGVVKKLNVEAPGKFEVSS
                     AETMLSQL"
     gene            2020..3816
                     /locus_tag="VI319_04940"
     CDS             2020..3816
                     /locus_tag="VI319_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY6863225.1"
                     /translation="MQTAAETVQEHRLTLSEVMQALVADGLVPKAEADRLVADRRLHR
                     ADLHPLAVVADQKWRSGRPPHKPLSLEWLTQWLAEKAGMDYYHIDPLKINFSAVTEVM
                     SNAYAARFRILPVEVTTKEAVVATCEPYVKEWEKELKPILKLDIRRVIANPLDIARYQ
                     VEFYNLAKSVKGAAKQGEQRSGLGNFEQLVELGKTKGQLDANDQHIVHVVDWLWQYAF
                     DQRASDIHMEPRRDVAEVRFRIDGVLHPVYQIPGAVAAAMTSRIKILGRMDVVEKRKP
                     QDGRIKTRTADGLEIELRLSTLPTAFGEKLVMRIFDPEVIVKDFAELGFSEDDKTRWQ
                     HMTEQPNGIILVTGPTGSGKTTTLYSTLKSLATPEVNVCTIEDPIEMIEPTFNQMQVQ
                     PAIELGFAQGVRALMRQDPDIIMVGEVRDLETADMAVQAALTGHLVLSTLHTNDAASA
                     ITRLLDLGVPAYLLNSTILGVMAQRLVRTLCRHCKEKIPYDSDREGELSWDEFVAPWK
                     AKRPSHLYRPVGCLECRNTGYLGRVGLYEILLLSPTIRKLVTSEADVARIREQAYKEG
                     MKPLRISGALKVAMGVTTLDEVLKVAPPPGQA"
     gene            complement(3818..4192)
                     /locus_tag="VI319_04945"
     CDS             complement(3818..4192)
                     /locus_tag="VI319_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEY6863226.1"
                     /translation="MLSKVTIALALAVAAQVASAATVSPKSQPRNAWGAIAYSSQTGA
                     YGFAVDQPSKRSAESEAFRQCGADCDVIKTFRNTCGAVAQSRTRYVWDTGASREIAEM
                     KAQKRCGGDSCRTLVWACTRGK"
     gene            complement(4250..5425)
                     /locus_tag="VI319_04950"
     CDS             complement(4250..5425)
                     /locus_tag="VI319_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6863227.1"
                     /translation="MLTGFLLKLKSHKLPVSIKEWLTLLEAMRKGVISPSIDDFYHLS
                     RTALVKDEQNFDKFDRAFGEYFKGVESIVGPELDVPLEWLLKQAELNLSAEEKAMIEA
                     LGGWEKLMEELKKRLEEQKGRHQGGNKWIGTAGTSPFGAYGYNPEGVRIGQERSRNRS
                     AVKVWDARESRNLDDSVELGTRNIKVALRRLRKFAREGAPEELDLDTTIDKTARNAGW
                     LDLHMRAERHNAVKVLMFLDIGGSMDDHVKLCEELFSASKTEFKHLEYFYFHNCLYDY
                     VWKDNRRRHAERVRTFDIMHKYGHDYKVIFVGDATMSPYEILQPGGSIEYSNDEAGAV
                     WMKRMLDVYPKAAWLNPEPEEIWPYRQSVGILREIMGGRMYPTTIEGLERAMRSLAK"
     gene            complement(5430..6299)
                     /locus_tag="VI319_04955"
     CDS             complement(5430..6299)
                     /locus_tag="VI319_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6863228.1"
                     /translation="MNPSRKNPMRFKGSDTYVATDDLRLAVNAAITLERPLLVKGEPG
                     TGKTMLAVEVARALGLPLLEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDPRVQDIANY
                     IVRGTLWQAFVSETPVVLLIDEVDKADIEFPNDLLRELDRMEFYVYETRELVKARHRP
                     VVFITSNNEKELPDAFLRRCFFHYIRFPDKDTMEKIVDVHFPGLKKSLLREALEVFFE
                     VREVPGLKKKPSTSELLDWLKLLLAEDISPEALRSKDRKTIIPPLHGALLKNEQDVHL
                     FERLIFMSRQQRS"
     gene            6344..7063
                     /locus_tag="VI319_04960"
     CDS             6344..7063
                     /locus_tag="VI319_04960"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY6863229.1"
                     /translation="MRYAALLLLMLVTRAHCAVVAVDVGHFNAEPGTISARGRPEFEF
                     NRDLALEVRAALAARGLDAKLIGADGTMAHLSRRTATAAADGADFLLSVHHDSTQPQF
                     FLSWNDGGVDRSYSDRYAGFALFVSRRNVDPAASLRCASGIGAALRRAGFTPSLYHAE
                     PIPGESKPFADRENGVHYYDNLVVLHTARSPAVLLEAGVIVNRAEETKLASPATRKRI
                     AAAVAEGVGRCLGPRAGASEF"
     gene            7138..7485
                     /locus_tag="VI319_04965"
     CDS             7138..7485
                     /locus_tag="VI319_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6863230.1"
                     /translation="MKKCLGMLLAVASACAFAQQSAPERSGEKAGSTPRFQSMCVGCH
                     GIPYYQTAFPQVYHVPKIAGQSRQYIVNALKAYKAGDRSHPSMQGIAKSLSDEDMAAL
                     AAYYGADPAEAKK"
     gene            7482..7805
                     /locus_tag="VI319_04970"
     CDS             7482..7805
                     /locus_tag="VI319_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6863231.1"
                     /translation="MSKLLALVLAASVSVPALAAGNAEAGKQKSQACAACHGADGNHP
                     TTPDFPRLAGQHPDYLLKALRDYKSGARKNPIMGGQVQALKDEDLADLAAYFSEQSGT
                     LHVKR"
     gene            complement(7812..8234)
                     /locus_tag="VI319_04975"
     CDS             complement(7812..8234)
                     /locus_tag="VI319_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEY6863232.1"
                     /translation="MFNPSRDQVRQFFFDAWRKYGDRSPLTGLEPMAVDVMLAHPEYR
                     DALEHPERYVVREYTPESGESNPFLHLSLHLAIEEQISIDQPPGIRAEFERIARARGD
                     RHEALHAVLECLGETVWRAQRDAAAPDAAAYLECLRKR"
     gene            complement(8237..8881)
                     /gene="nth"
                     /locus_tag="VI319_04980"
     CDS             complement(8237..8881)
                     /gene="nth"
                     /locus_tag="VI319_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY6863233.1"
                     /translation="MNPVKRREIFSRFRAANPSPTTELVYRTPFELLVAVILSAQATD
                     KSVNLATGKLFHDANTPEKMLALGESGLSEYVRTIGLYRGKAKNIIQTCRILIEQHGG
                     RVPESRDALEALPGVGRKTANVVLNTAFGHPTIAVDTHIFRVANRTGIAPGKDVREVE
                     DKLMKFVPEEFCKDAHHWLILHGRYVCKALKPDCPRCLIRDLCEYRPKTREARA"
     gene            complement(8878..9474)
                     /locus_tag="VI319_04985"
     CDS             complement(8878..9474)
                     /locus_tag="VI319_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEY6863234.1"
                     /translation="MSAATVDRIDALLPQTQCGKCGHAGCRPYAQAIAAGAAINRCPP
                     GGEATLRNLAELTGRDAAPLDPACGAETPRRVARIEETRCIGCTLCIQACPVDAIVGA
                     PQRMHTVLTELCSGCELCLAPCPVDCIALVLPAPGDVWTRARADAARDRHEWRNARVA
                     RDRREQARRHSATARKRAVVRAAIERARARRAAAGRAR"
     gene            complement(9471..>9755)
                     /locus_tag="VI319_04990"
     CDS             complement(9471..>9755)
                     /locus_tag="VI319_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY6863235.1"
                     /translation="KHLRHGDEAGGLSGPPLRARATQVVAKLRRALPARVSIIGVGGI
                     ASARDAREKLDAGADLVQVYTALLYRGPRLAGEIVRGLAADTRKAQGAAA"
BASE COUNT         1527 a         3315 c         3295 g         1618 t
ORIGIN      
        1 accgcgtcgg gccgggcaag cggccgcgca gctcgatggc gccgacgctg gtgttcgacg
       61 ccgagggcga cgcggtgagc gtcaccgcga gcatcgagtc ggcgttcgga aaccggcaga
      121 tggtgcgcgg cttcatgctg aacaacgagc tcaccgattt ctcgtggctg cccgaggccg
      181 acggcaagcc ggtcgcgaac cgcgtcgggc cgggcaagcg gccgcgcagc tcgatggcgc
      241 cgacgctggt gttcgacgcc gagggacgcc tgctgctcgc gatcggatcg ccgggcggtc
      301 actcgatcat caattacgtc gcgaagacgc tggtcggggt gctcgactgg gggctgtacg
      361 tgcaggacgc gatctcgttg ccgaatttcg gcagccgcaa cggcccgacc gacctggaac
      421 gcggcacgcc gctggagcgg ctggtcccgc agctccgcgc catgcagcac gtcgtgcgca
      481 tccggcccga gcccagcgga ctgcatggta tacaacgggt ccccggcgga tggctcggag
      541 gagcggaccc gcggcgggag ggcgccgccg tgggggaatg agacgcaggt cacggcgggc
      601 gatggaatac tgccggagct cgccgcgtac gctccataat gtccccgctc ttaaccccaa
      661 gggagccctc atgaagatcg gaaagatcgt ttccgtgatt gcggtcctcg ccgcggcggg
      721 cgcggcgcac gccgaagaag gcatcggtct gcgcgtcggc acgctcggct acggcggcga
      781 cttcggctgg cacatcgcgc ccgggctgaa cgcgcgcctc ggttactcga cgtactcgca
      841 caacacgagc atcaccaata cggacgtcca ctacgacggc aagctgaagc tttctaatgc
      901 gccgctgctg ttcgacttca gcccgatcgg ccccttccac ttcacggcgg gcatcatcgg
      961 caatgccaac aagatcgaca ttaccggtac gccggcctcg aacggcacgt tcacgttcaa
     1021 cggtcagacg ttcacttcga gccaggtcgg cagcgtctcg ggacggctca agctgggcaa
     1081 cagcgtcgcg ccttatctcg ggatcggttg gggcaacgtc gccggcgctg gagtcaactt
     1141 ctatgccgac ctcggcgtcg cgtacatggg atcgccgaag gcgtcgctga gcgcgacgtg
     1201 cgggtccggt gtgccggcga gcacgtgcgc gcagatccag gccgacgtcg aggccgagca
     1261 gcgcgccctc gaggacaagg tcaagaagta caaatactac cccgtgctcg gtatcggtct
     1321 cacgatcggc ttctgagcgg cggcccccga aaagaaaaac gcccggcatc gccgggcgtt
     1381 tttctttcgc gctcgcgcgt cagagctggg agagcatcgt ctcggcgctg ctgacctcga
     1441 acttgcccgg cgcctcgacg ttcagcttct tcaccacgcc gttgtcgacg agcatcgagt
     1501 agcgctgcga gcgcaggccc atgttgcgcg cggagagatc gagctcgagc ccgagcttct
     1561 tcgtccattc ggcgctgccg tcgccgagca tgcggatctt gccgccggcc ttctggtcgc
     1621 ggccccaggc cgccatcacg aagccgtcgt tggtcgcgac gcaccagatc tcgtcgacgc
     1681 ccttgctctt cagcttgtcg tagttcttca cgtagcccgg cacgtgcttg gcggagcacg
     1741 tcggcgtata cgcgcccggc agcgcgaaga tcacgatctt cttgcctttg acggaatcgg
     1801 ccacgctgat gtccttgggc ggcatcgggc agcccgcggc ctcgtcgaat tcggtggatt
     1861 cggaaagctt gccgtcgggc agcttgtcgc ccggtttgat cgccatgggg gtctcctctc
     1921 gaggttgttc gtctgcttgg agggacgcta gcatgtcgca gttcccatgc gctgtcaaaa
     1981 tggcttgctc gccccccggg gcgtggctat attgccgcca tgcaaaccgc cgccgaaacg
     2041 gtccaggagc acaggctcac gctgtcggag gtgatgcagg ctctcgtcgc cgacgggctc
     2101 gtgccgaaag ccgaggccga caggctcgtg gcggaccgcc ggctgcaccg cgcggacctg
     2161 cacccgctcg ccgtcgtcgc cgatcagaaa tggcgctcgg gacgtcctcc gcacaagccg
     2221 ctgtcgctcg agtggctgac gcagtggctc gcggagaagg cggggatgga ctactaccac
     2281 atcgatcccc tcaagatcaa tttctcggcc gtgaccgagg tgatgtcgaa cgcgtacgcg
     2341 gcgcgcttcc gcatcctgcc ggtcgaggtg accacgaagg aggcggtcgt cgcgacctgc
     2401 gagccgtacg tcaaggaatg ggagaaggag ctcaagccca tcctgaagct cgacatccgc
     2461 cgcgtgatcg ccaacccgct cgacatcgcg aggtaccagg tcgagttcta taacctcgcg
     2521 aaatcggtga agggcgcggc caagcaaggg gagcagcgca gcggcctcgg caacttcgag
     2581 cagctggtcg agttgggcaa gaccaagggc cagctcgacg cgaacgacca gcacatcgtc
     2641 cacgtggtcg actggctctg gcagtacgcc ttcgaccagc gcgcgagcga cattcacatg
     2701 gagccgcggc gcgacgtcgc cgaggtccgc ttcaggatcg acggcgtgct gcacccggtg
     2761 taccagatcc cgggcgccgt cgcggcggcg atgacctcgc gcatcaagat cctcgggcgc
     2821 atggacgtag tggagaagcg caagccccag gacgggcgca tcaagacccg gaccgcggac
     2881 ggcctcgaga tcgagctgcg cctctcgacc ctgccgaccg cgttcggcga gaagctggtc
     2941 atgcgcatct tcgatcccga ggtgatcgtc aaggacttcg ccgagctggg gttctccgag
     3001 gacgacaaga cgcgatggca gcacatgacc gagcagccga acggcatcat cctcgtgacc
     3061 ggccccaccg ggtcgggcaa gacgacgacg ctgtactcga cgctgaagag cctcgccacg
     3121 ccggaagtga acgtctgcac catcgaggac ccgatcgaga tgatcgagcc gacgttcaac
     3181 cagatgcagg tgcagccggc gatcgagctc ggcttcgcgc agggcgtgcg cgcgctcatg
     3241 cgccaggacc cggacatcat catggtgggc gaggttcgcg acctcgagac cgccgacatg
     3301 gcggtccagg cggcgctcac cggtcacctc gtgctgtcga ctctgcacac caacgacgcg
     3361 gcgagcgcga tcacgcggct gctcgacctt ggtgtgccgg cgtaccttct gaactccacg
     3421 atcctcggcg tcatggcgca gcgcctggtg cgcacgctct gccgtcattg caaggaaaag
     3481 attccctacg acagcgaccg cgagggcgag ctcagctggg acgagttcgt ggcgccgtgg
     3541 aaggccaagc ggccttcgca tctatatcgg ccggtcggct gcctggaatg ccgcaacacg
     3601 ggctacctcg gccgcgtggg cttgtacgag atactcctgc tcagtccgac gatccgcaag
     3661 ctcgtgacct ccgaggccga cgtcgccagg atccgcgagc aggcctataa ggaaggcatg
     3721 aagccgctgc gcatcagcgg agcgctgaaa gtcgcgatgg gcgtgaccac tctcgacgag
     3781 gtgctgaagg tcgctccgcc gcccggccag gcctgagcta ttttccccgg gtgcaagccc
     3841 acacgagcgt gcggcagctg tcgccgccgc atcgcttctg cgccttcatc tccgcgatct
     3901 cgcgcgaggc gccggtgtcc cagacgtagc gcgtccgcga ctgcgcgacc gcgccgcacg
     3961 tattcctgaa cgtcttgatg acgtcgcagt cggccccgca ttgccggaag gcttcgctct
     4021 cggcggagcg cttcgagggc tggtcgacgg cgaagccgta ggcgccggtc tggctgctgt
     4081 aggcgatcgc gccccaggcg ttgcgcggct ggctcttcgg cgagacggtg gccgcggacg
     4141 cgacctgggc ggcgacggcc agcgccagcg cgatggtgac tttgctcagc atgggcgcga
     4201 agcatacacc ccgcgcggcc ggaaagcagc cggcgttggc tcgccgccgc tacttcgcca
     4261 ggctgcgcat cgcgcgctcg agtccttcta tcgtggtcgg atacatccgc ccgcccatga
     4321 tctcgcgcag gatgccgacc gactggcgat agggccagat ctcctcgggc tcgggattga
     4381 gccacgcggc cttggggtag acgtccagca tgcgcttcat ccagacggcg cccgcctcgt
     4441 cgttcgaata ctcgatcgag cctcccggct gcaggatctc gtacgggctc atggtcgcgt
     4501 cgccgacgaa gatcacctta tagtcgtggc cgtacttgtg catgatgtcg aacgtgcgca
     4561 cccgctcggc gtggcgccgg cgattgtcct tccatacgta gtcgtacagg caattgtgga
     4621 agtagaagta ctcgaggtgc ttgaactcgg tcttcgaagc ggagaagagc tcctcgcaca
     4681 gcttgacgtg gtcgtccatc gagccgccga tgtcgaggaa catgagcacc ttcacggcgt
     4741 tgtgccgctc ggcccgcatg tgcaggtcga gccagccggc attgcgcgcc gtcttgtcga
     4801 tggtcgtgtc gaggtcgagc tcctcgggcg cgccttcgcg cgcgaacttg cgcagccggc
     4861 gcagcgccac cttgatgttg cgcgtgccga gttccaccga gtcgtcgagg ttccgggact
     4921 cacgcgcgtc ccagaccttg accgcgctac ggttgcgcga tcgctcctgg ccgatcctca
     4981 cgccctcggg gttgtagccg taggcgccga aaggcgaggt gcccgcggtg ccgatccact
     5041 tgttgccgcc ctggtgccgg cctttctgct cctccagccg cttcttcagc tcctccatca
     5101 gcttttccca gcccccgagc gcctcgatca tcgccttctc ctcggccgat aggttgagct
     5161 cggcctgctt cagcagccac tcgagcggca cgtcgagctc gggaccgacg atcgactcga
     5221 cgcctttgaa gtactcgccg aaggcgcggt cgaacttgtc gaagttctgc tcgtccttca
     5281 cgagcgcggt gcgcgagaga tggtagaagt cgtcgatcga cggcgagatc acgcccttgc
     5341 gcatcgcctc gaggagcgtc agccactcct tgatcgagac cggcagcttg tggctcttga
     5401 gcttcagcag gaagccggtg agcacggcct cagctccgct gctgcctcga catgaagatc
     5461 agccgctcga acaggtgcac gtcctgctcg ttcttgagca gcgcgccgtg aaggggcggg
     5521 atgatcgtct tccggtcctt gctgcgcagg gcctcgggtg agatgtcctc ggcgagcagc
     5581 agcttcagcc agtcgagcag ctcagacgtc gagggcttct tcttcaggcc cgggacctcg
     5641 cgcacctcga agaagacctc gagcgcctcg cgcagcagcg acttcttcag ccccgggaaa
     5701 tggacgtcga cgatcttctc catcgtgtcc ttgtccggga agcggatgta gtggaagaag
     5761 cagcgcctga gaaaggcgtc gggaagctcc ttttcgttgt tgctcgtgat gaagacgacc
     5821 gggcggtgcc gggccttgac cagctcgcgc gtctcgtaga cgtagaactc catgcggtcg
     5881 agctcgcgca gcaggtcgtt cgggaattcg atgtccgcct tgtcgacttc gtcgatcagc
     5941 agcacgaccg gcgtctcgga gacgaaggcc tgccagagcg tgccgcgcac gatgtagttg
     6001 gcgatgtcct gcacgcgcgg gtcgccgagc tgcgagtcgc gcaggcggga caccgcgtcg
     6061 tattcgtaca gcccctgctg cgccttggtc gtggacttga tgtgccattc gagcagcggc
     6121 aggccgagcg cgcgcgcgac ctccaccgcg agcatcgtct tgcccgtccc gggctcgccc
     6181 ttgaccagca gcggccgctc gagcgtgatc gcggcgttga cggcgagcct caggtcgtcg
     6241 gtcgcgacgt aggtgtcgga gcctttgaat cgcatcgggt tctttcgtga cgggttcatt
     6301 ctacatcctt gctcccggcc gtccgctgga cgagaatggc gccatgcggt atgcagcgct
     6361 gctgttattg atgctggtca cgcgcgcgca ttgcgccgtc gtcgccgtgg acgtcggcca
     6421 cttcaacgca gaaccgggaa cgatcagcgc gcgcgggcgt ccggagttcg agttcaatcg
     6481 cgacctcgcg ctcgaggtgc gggcggcgct cgcggcccgg ggcctcgacg cgaagctcat
     6541 cggcgccgac ggcacgatgg cgcacctgtc gcgtcggacg gcgacggccg cggccgacgg
     6601 cgccgatttc ctgctttcgg tgcaccacga ttcgacccag ccgcagttct tcctcagctg
     6661 gaacgacggc ggcgtcgacc ggtcctacag cgaccgctac gccggattcg cgctgttcgt
     6721 gtcgcggcgt aacgtcgacc ccgccgcgag cctgcgctgc gcttcgggga tcggcgctgc
     6781 gctgcgccga gcggggttca ccccttccct ctaccacgcc gagcccattc cgggcgagag
     6841 caagccgttc gccgaccgcg agaacggcgt gcactactac gacaatctcg tggtgctgca
     6901 tacggcgcgc tcgcccgcgg tgctgctcga ggccggcgtc atcgtgaacc gcgccgagga
     6961 gaccaagctc gcgtcgccgg cgacgcggaa gcgcatcgcc gccgcggtcg ccgaaggcgt
     7021 cggccgctgc ctcgggccgc gtgcgggcgc ttcggagttt tgaccgggga acgggcctcg
     7081 ttatagaatt gcgcggcctc catacgcttc tgccgccgaa taatgaaaat cccgacgatg
     7141 aagaaatgcc tcggcatgct gctcgcggtc gcctccgcat gcgcgttcgc gcaacaatcg
     7201 gcgccggagc gctccggcga gaaggcggga agcaccccgc gcttccagtc gatgtgcgtc
     7261 ggctgccacg gcatcccgta ctaccagacc gccttcccgc aggtctacca cgtgccgaag
     7321 atcgcggggc agtcgcggca gtacatcgtc aacgcgctga aggcgtacaa ggcgggggac
     7381 cgcagccacc cgtcgatgca gggcatcgcg aaaagcctga gcgacgagga catggcggcg
     7441 ctcgccgcct actacggggc cgacccggcg gaggccaaga aatgagcaag ctcctcgccc
     7501 tcgtcctcgc cgcatccgtg tcggttcccg cgctcgccgc cggaaacgcc gaggccggca
     7561 agcagaaatc ccaggcctgc gccgcgtgcc acggcgccga cggcaaccac ccgacgacgc
     7621 ccgattttcc gcgcctcgcc gggcagcatc cggattacct gctgaaggcg ctgcgcgact
     7681 acaagtcggg cgcgcgcaag aatcccatca tgggcgggca ggtccaggcc ctcaaggacg
     7741 aggacctcgc cgacctcgcg gcgtacttct cggagcagag cggcaccctg cacgtcaagc
     7801 gctgagccgg ttcagcgctt cctgagacat tcgagatagg cggccgcgtc cggcgcggcc
     7861 gcgtcgcgct gcgcccgcca taccgtctcg ccgaggcatt cgagcaccgc gtgcagcgcc
     7921 tcgtgccggt cgccgcgggc gcgcgcgatg cgctcgaact cggcgcggat ccccggcggc
     7981 tggtcgatcg agatctgctc ctcgatcgcg agatgcagcg agaggtgcag gaagggattg
     8041 ctctcgcccg attcgggcgt gtactcgcgc acgacgtagc gctccgggtg ctccagcgcg
     8101 tcgcgatact ccgggtgcgc gagcatgacg tcgacggcca tcggctccag gccggtgagc
     8161 ggcgagcggt cgccgtactt gcgccaggcg tcgaagaaga actggcgcac ctggtcgcgc
     8221 gaaggattga acatcgtcag gcgcgggctt cgcgcgtctt gggacggtac tcgcagaggt
     8281 cgcggatcag gcacctcggg cagtcgggct tcagcgcctt gcagacgtag cgcccgtgca
     8341 ggatcagcca gtggtgcgcg tccttgcaga actcttcggg cacgaacttc atcagcttgt
     8401 cctcgacctc gcgcacgtcc ttgcccggcg cgatgccggt gcggttcgcg acgcggaaga
     8461 tgtgcgtatc gactgcgatg gtcgggtgcc cgaacgcggt gttcagcacg acgttcgccg
     8521 tcttgcggcc gacgccgggc agggcctcga gcgcatcgcg cgactcgggg acccggccgc
     8581 cgtgctgctc gatcaggatg cggcaggtct ggataatgtt cttcgccttg ccgcggtaca
     8641 ggccgatggt gcggacgtat tcggagaggc cgctctcgcc gagcgcgagc atcttctcgg
     8701 gcgtgttcgc atcgtgaaac agcttcccgg tcgcgagatt cacgctcttg tcggtcgcct
     8761 gcgccgagag gatcaccgcc accagcagct cgaacggggt gcggtagacg agctcggtcg
     8821 tcggcgacgg gttcgccgcc cggaagcgcg agaagatctc gcgccgcttg acaggattca
     8881 tcgcgcgcgt ccggcggcgg ccctgcgggc gcgcgcgcgc tcgatcgcgg cgcgcacgac
     8941 cgcccgcttc ctggcggtgg ccgagtggcg ccgcgcctgc tcgcggcggt cgcgcgcgac
     9001 gcgtgcgttg cgccattcgt ggcggtcgcg cgcggcgtcc gcgcgggctc gcgtccacac
     9061 gtcgccgggc gcgggcagga ccagcgcgat gcagtcgacg gggcacggtg cgaggcacag
     9121 ctcgcatccg gagcacaact cggtgagcac cgtgtgcatg cgctgcggcg caccgacgat
     9181 ggcgtcgacc gggcacgcct ggatgcacag cgtgcagccg atgcagcgcg tttcctcgat
     9241 ccgcgcgacg cgccgcggcg tctccgcgcc gcacgcggga tcgagcggcg cggcgtcgcg
     9301 cccggtcagc tccgcgagat tgcgcagggt ggcctcgcct ccgggcgggc agcggttgat
     9361 cgccgcgccg gcggcgatcg cctgcgcata gggccggcac cccgcgtggc cgcatttgcc
     9421 gcactgcgtc tgcggaagca gcgcatcgat ccggtcgacc gtcgccgcgc tcacgcggcc
     9481 gctccttgcg ccttccgcgt gtccgccgcg agcccgcgga cgatctcgcc cgccagccgc
     9541 gggccccggt acagcagcgc cgtatatacc tgcaccaggt cggcacccgc gtcgagcttc
     9601 tcgcgcgcgt cgcgggcgct cgcgatgccg cccacgccga tgatcgagac gcgtgccggc
     9661 agcgcgcggc gcagcttcgc cacgacctgc gtggcgcgcg cgcgcagcgg cgggcccgag
     9721 agcccgcccg cctcgtcgcc gtggcgcaag tgctt
//
LOCUS       DATDMW010000111         8646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1276090, whole genome shotgun sequence.
ACCESSION   DATDMW010000111 DATDMW010000000
VERSION     DATDMW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276090"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VI319_04995"
     CDS             <1..617
                     /locus_tag="VI319_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6863236.1"
                     /translation="QVEDTRSALACLAARPEIDPARIAVMGHSFGAAVAVYAAGVDAR
                     FAACISTGGWGDGESKFRAQHASPEAWTRFSAMMDEGRRRKARGESMQVPRYEIVPIP
                     PGLRGNLAPGSIMEFPFEVVESMYTFRANDVVRRIAPRPLLLLHPARDSVTPTEQSIE
                     LFRRAGQPCDLHLVADVDHFMLSESNTLVLGIVRDWLKKYFPAS"
     gene            614..1669
                     /locus_tag="VI319_05000"
     CDS             614..1669
                     /locus_tag="VI319_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY6863237.1"
                     /translation="MSGVRVVSADALRDFAAAVLARTGLSAPRAGEVADALAWANLRG
                     IDTHGVTRLPRYVDLLQSGDMNPAPNLRVVGETAAAVVIDADRAPGPIAMNEAMAAAS
                     RKARAAGIGLGLARATTHTAALGYYTLAAARDGMAAIALAASWPNMAYHGARAAGVST
                     SPISIAVPGGREPLVLDMGTGVVSVGRLTQARKSGTPIPEGWALDADGRPTTDPQKAD
                     LPLPMAGPKGSGLALMIECLTSLVVANPLISEFLEETPLGLRHRQNGLAIAIDVSRFG
                     DPAAFRREVERTVRALKALPRGDGVDEILVPGERGARAFAQRMRDGVPIPPPVWKELA
                     ALAERLGVAMMTIQGDS"
     gene            1671..2666
                     /locus_tag="VI319_05005"
     CDS             1671..2666
                     /locus_tag="VI319_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6863238.1"
                     /translation="MSGPIDVHCHFVPSQFPPYAGRDPKARWPGMQHDGCGHANVVIQ
                     GKNYRTVVEQAWDGAKRVVDMDRMGIARQVVSPMPELLSYWLGVEDAKVLVRHVNDEI
                     AALCAANPRRFSGLGGVPLQDVDAAMRELEHVLRSQKLLGVEIATHVNGVSIGDARFE
                     PFFAEAERLGAPIFVHALHPAGLDRLVGPPALEQVVAFPGDVALGIASMITGGTLERH
                     PKLKIGFSHGGGAFAQVLPRLEHGWKMIPAIRDKIPQAPSAYARKLYYDDLVYDAKSL
                     AFVIERFGATQIMIGTDYPFNVQDKDPLGSLAAAGLDEACARLLREDNARRFLGL"
     gene            2641..3675
                     /locus_tag="VI319_05010"
     CDS             2641..3675
                     /locus_tag="VI319_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEY6863239.1"
                     /translation="MPGASWGSSVRLAAALALSLALAPAFGKTPADCAVLDSDRQRLQ
                     CYDELAKPGAEIADRNSYLVRSWKLGNVEPPRLGIIEEYRPTYFLLWRKSNDPNTMPS
                     TPAPGHALAEPLDLDAQEAKFQLSFKTELARSTRVDVATANEYGFHRFRLWAAYTQQS
                     SWQWFNSRDSRPFRETDYEPELIATFDRHVAGAGLKMVNIGFAHQSNGRDDPFSRSWN
                     RVYLQGGWEWPVRRVAVMARVWARVDSRRDDDNPDITKYLGHGDVVARWESQENCAVD
                     AANDRCHRVWLTMRPNPGHGFVQIDWATAWRIGLARFHVQVTSGYGESLIDYNHSQTT
                     YGFGFSVGDW"
     gene            complement(3676..4275)
                     /locus_tag="VI319_05015"
     CDS             complement(3676..4275)
                     /locus_tag="VI319_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY6863240.1"
                     /translation="MSALPKDVHPETRSRLSPLARGQLDEAGKRLYDSVLDPKRPTIA
                     GFQGPAGIWLHSPRVGEPIREFNRILRTEAGLEPRLRELAILVTARELDSQFEWSAHE
                     PVALREGLAPAVIDAVKHRKPAGKMDEREAAIIRFGRELFRDRRVRPETYAEALRLFG
                     ETGLVNLAALMANYALTAAMLAAFDQQLHEGQEPLLPLA"
     gene            complement(4272..5249)
                     /locus_tag="VI319_05020"
     CDS             complement(4272..5249)
                     /locus_tag="VI319_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863241.1"
                     /translation="MAHSWRWVAIAAVALGAHATAWGQATAAWPTKPIRVIVPFTPGS
                     GTDIMARTVSEGLSKRLGQPLIVENRPGAGGTIGEAIVAKAEPDGHTLLVHSSSYTVT
                     PSTYKDLPFDTERDLMGIVPLGQLPNVLVISPSKGIHSVKELVAAARKKPGAMNSASV
                     GVGSATHLNAERFRLGAGIDVVNIPFKGTPEALTEVIAGRVDYYFCPVNAALPMIKDG
                     KVLALAVGSSERSKALPDLPTTLEAGVLNSDYNFWVGMFGPAKMKRDVVARLYDESVK
                     VLQSPDVREKMSRLGAEPMNYTPGRFNLYLQGEIRANAELVRKAGIKAQ"
     gene            5337..7481
                     /locus_tag="VI319_05025"
     CDS             5337..7481
                     /locus_tag="VI319_05025"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6863242.1"
                     /translation="MLGWLLKSRRNKGDAAAAGAHVSAGLQHHQAGRLRDAEREYAEA
                     LGLDPECIDALHFSGVIAYQQERHDDAQRLISRALSLDAANPPAHNNLGNVLLKQGRL
                     EDALASFERAIALQPDYLDAHVNLASVWLERARPERALECIGRALRSLPGAARLRLQR
                     AQALAALGRHEEAAAAFREAIEADPASAAAYSGLGHALGHAGRPDEAIAAYRRAAALD
                     PGGAGPRYDAGVVCHGIGRTDEALECFGEALAVDPEFAPARWALTTGQLPAVYADAAE
                     PELRRAAFARELATLERWVAPERLAASVHAVGAQQPFYLAYQDRNNRELLGRYGALCA
                     RVMDAWLREERPASAARAARDRVRVGVVSAHFHNHSVWHAIIKGWLLQIDPDRFELDV
                     FHLGSTHDAETDLARSRAAAFHAGPLTLRQWTEAILERRPDVLLYPDVGMEPVTVKLA
                     SLRLAPVQAAAWGHPETTGLPTVDCYLSGLDMEPAGADAHYTERLVALPHLGCWYEAT
                     GVEPEAPPVAVDVPPGAPLLVCPGVPFKYAPEHDAALAGIARALGRCRFVFFTHRPPE
                     LSERLRLRLEAAFRREGLDFRDFGVFAPWLPRGAFLGMLKAADAMLDTIGFSGFNTAM
                     QAVEAGLPIVTVEGRFMRGRFASAILRRMEISELVARSADEYVAVATKLGRDAAYRRG
                     LRERIEARRGILFGDRAPILALEAFLAEAAGR"
     gene            7529..8404
                     /locus_tag="VI319_05030"
     CDS             7529..8404
                     /locus_tag="VI319_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY6863243.1"
                     /translation="MKAAPFEYSRAASIAEACDLLARHGGDAKLLAGGQSLVPMMAMR
                     LLRPAALIDINEIAALKGVARDERFARIGACTRQVAVERDDALAASVPLLRRALAWVG
                     HVQTRNRGTVGGSLAHADPSAELPLAAQVLGAQIALRSKSGTRTLAADAFFAGPMMTA
                     ARPDECIEEIRWPVWPERRTGCAFTEVSVRHGDFAIVAAAAQVALDADGRCARASFGL
                     GGAGGTPLAFPKIAALLVGTKLEDDALEDAAKAAAAGCDPGTDLHATADYRRHLARVL
                     VARVLREARDGAKAR"
     gene            8401..>8646
                     /locus_tag="VI319_05035"
     CDS             8401..>8646
                     /locus_tag="VI319_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6863244.1"
                     /translation="MSETLHSISVEVNGVRREARVPARLSLVDWLRDELRLTGTHVGC
                     EHGVCGACTVLVDGTSIRSCLMFAVQANGRSVMTVEAL"
BASE COUNT         1174 a         3096 c         3115 g         1261 t
ORIGIN      
        1 agcaggtcga ggacacgcgc agcgcgctcg cgtgcctcgc ggcgcggccg gagatcgacc
       61 cggcgcggat cgccgtcatg gggcacagct tcggcgccgc cgtcgccgtc tacgccgcag
      121 gcgtggacgc gcgcttcgcc gcgtgcatat cgaccggcgg ctggggcgac ggcgagtcga
      181 agttccgcgc ccagcacgct tcgcccgagg cctggacgcg cttctccgcc atgatggacg
      241 aggggcgccg gcgcaaggct cgcggcgaat cgatgcaggt gccgcgctac gagatcgtgc
      301 cgatcccgcc cgggctccgc ggcaacctcg ctccgggctc gatcatggag ttcccgttcg
      361 aggtggtgga gagcatgtat acgttccggg cgaacgacgt ggtccgccgg atcgcgccgc
      421 ggccgctgct gctcctgcac ccggcgcgcg actcggtgac accgaccgag cagtcgatcg
      481 agcttttccg ccgcgcgggc cagccgtgcg acctgcacct ggtcgccgac gtcgatcatt
      541 tcatgctgtc cgagagcaac acgctggttc tgggcatcgt gcgcgactgg ctgaagaaat
      601 actttcccgc ctcatgagcg gcgtccgggt ggtgagcgcc gatgcgctgc gcgatttcgc
      661 cgcggcggtg ctcgcgcgca ccggcctgtc ggcgccgcgc gccggtgagg tcgccgacgc
      721 gctcgcgtgg gcgaacctgc gcgggatcga cacgcacggc gtgacgcggc ttccgcgcta
      781 cgtcgacctg ctgcagtccg gcgacatgaa tcccgcgccg aatctgcgcg tcgtgggcga
      841 gaccgcggcg gcggtcgtga tcgacgccga ccgcgcgccc ggcccgatcg cgatgaacga
      901 ggcgatggcc gcggcatcgc gcaaggcgcg cgccgccggc atcggcctcg ggctcgcacg
      961 cgcgaccacg cacaccgcgg cgctcggcta ctacacgctc gccgcagcgc gcgacggcat
     1021 ggcggcgatc gcgctcgccg cgtcgtggcc gaacatggcg taccacggcg cgcgcgcggc
     1081 cggcgtgtcg acgagcccga tctcgatcgc cgtccccggc gggcgcgagc cgctcgtgct
     1141 cgatatgggc acgggcgtgg tctcggtcgg acgcctcacg caggcgcgca agagcggcac
     1201 gccgatcccg gagggctggg cgctcgatgc ggacggccgg ccgacgaccg atccgcagaa
     1261 ggccgacctg ccgctgccca tggcggggcc gaagggctcg ggcctcgcgc tcatgatcga
     1321 atgcctgacg agcctcgtcg tcgcgaaccc gctgatctcc gagttcctcg aggagacgcc
     1381 gctcggcctg cgccaccgcc agaacggcct cgcgatcgcg atcgacgtgt cgcgcttcgg
     1441 cgacccggcg gcgttccggc gcgaggtcga gcgcacggtg cgcgcgctca aggccttgcc
     1501 ccgcggcgac ggcgtcgacg agatcctcgt gccgggcgag cgcggcgcgc gcgccttcgc
     1561 gcagcgcatg cgcgacggcg tcccgatccc gccgccggtc tggaaggagc tcgccgcgct
     1621 cgccgagcgg ctcggcgttg ccatgatgac gatccaagga gacagctgaa gtgagcggcc
     1681 ccatcgacgt gcattgccat ttcgttccgt cgcagtttcc gccgtacgcc ggccgcgatc
     1741 ccaaggcgcg ctggccgggc atgcagcacg acggatgcgg ccacgcgaac gtggtcatcc
     1801 agggcaagaa ctaccgcacg gtggtcgagc aggcgtggga cggcgccaag cgcgtcgtcg
     1861 acatggaccg catgggcatc gcgcgccagg tcgtttcgcc catgcccgag ctgctgtcgt
     1921 actggctcgg cgtcgaggac gcgaaggtgc tggtccggca cgtgaacgac gagatcgcgg
     1981 cgctgtgcgc ggcgaatccg cggcggttct cgggcctcgg cggggttccg ctgcaggacg
     2041 tcgacgccgc gatgcgcgag ctcgagcacg tcttgcgatc gcagaagctg ctcggcgtgg
     2101 agatcgcgac gcacgtgaac ggcgtgtcga tcggcgacgc gcgcttcgag ccgttcttcg
     2161 ccgaggccga gcgcctcggg gcgccgatct tcgtgcacgc gctgcacccg gcgggcctcg
     2221 accggctcgt ggggccgccc gcgctcgagc aggtggtcgc gttcccgggc gacgtcgcgc
     2281 tcgggatcgc gtcgatgatc acgggcggca cgctggagcg ccatccgaag ctgaagatcg
     2341 ggttctcgca cggcggcggg gctttcgcgc aggtgctgcc gcgcctcgag cacggctgga
     2401 agatgatccc cgcgatccgc gacaagatcc cgcaggcgcc gagcgcctac gcgaggaagc
     2461 tctattacga cgacctggtc tacgacgcga agagcctcgc tttcgtcatc gagcgcttcg
     2521 gcgccacgca gatcatgatc ggcaccgact acccgttcaa cgtgcaggac aaggatccgc
     2581 tcggctcgct cgcggccgcc ggcctcgacg aggcgtgcgc ccggctgctg cgcgaggaca
     2641 atgcccggcg cttcctgggg ctctagcgtc cggctcgccg ccgcgctcgc gctgtcgctg
     2701 gcgctcgcgc ccgcgttcgg gaagaccccg gccgactgcg ccgtcctcga cagcgaccgg
     2761 cagcgcctgc agtgctacga cgagctcgcg aagcccggcg cggagattgc cgaccgcaac
     2821 tcctacctcg tgcgctcgtg gaagctcggc aacgtcgagc cgccgcgcct cgggatcatc
     2881 gaggagtacc ggcccacgta cttcctgctc tggcgcaagt cgaacgaccc gaacacgatg
     2941 ccgagcacgc ccgcgcccgg ccacgcgctc gccgagccgc tcgacctaga cgcgcaggaa
     3001 gcgaagttcc agctctcgtt caagacggag ctcgcgcgct cgacgcgcgt cgacgtggcg
     3061 accgcgaacg agtacggctt ccaccgattc cgcctgtggg ccgcgtacac gcagcagtcg
     3121 agctggcaat ggttcaacag ccgcgactcg cggcccttcc gcgagaccga ctacgagccc
     3181 gagctgatcg cgacgttcga ccgccacgtc gccggcgcgg ggctgaagat ggtgaacatc
     3241 gggttcgcgc accagtcgaa cggccgcgac gatccgttct cgcgcagctg gaaccgcgtc
     3301 tacctgcagg gcggctggga atggccggtg cgccgcgtcg cggtgatggc gcgggtctgg
     3361 gcgcgcgtcg acagccgtcg cgacgacgac aaccccgaca tcacgaaata tctcgggcac
     3421 ggcgacgtcg tcgcgcgctg ggagtcgcag gagaactgcg cggtcgacgc cgccaacgac
     3481 cgctgccacc gcgtgtggct gaccatgcgc ccgaacccgg gccacggctt cgtgcagatc
     3541 gactgggcga ccgcgtggcg catcgggctc gcgcgcttcc acgtccaggt cacgagcggc
     3601 tacggcgaga gcctgatcga ctacaaccac tcgcagacga cctacggctt cggcttctcg
     3661 gtcggcgact ggtgatcagg caaggggcag cagcggctcc tgtccctcgt gcagttgctg
     3721 atcgaaggcg gcgagcatcg ccgcggtcag cgcgtagttc gccatgaggg ccgcgaggtt
     3781 caccaggccg gtttcgccga agaggcgcag cgcctcggcg taggtctccg ggcgcaccct
     3841 gcggtcgcgg aacagctcgc gtccgaagcg gatgatcgcg gcctcgcgct cgtccatctt
     3901 tccggcgggc ttgcggtgct tgaccgcgtc gatcaccgcg ggagccaggc cttccctgag
     3961 cgcgaccggc tcgtgcgcgc tccactcgaa ctggctgtcg agctcgcgcg ccgtgacgag
     4021 gatcgcgagc tcgcgcagcc gcggctcgag gccggcctcg gtacgcagga tgcggttgaa
     4081 ctcgcggatc ggctccccga cgcgcgggct gtgcagccag atgccggccg gaccctggaa
     4141 gccggcgatc gtcggccgct tcggatcgag caccgagtcg tagaggcgct tgcccgcctc
     4201 gtcgagctgc ccgcgcgcga gcggcgagag ccgcgatcgc gtctcgggat ggacgtcctt
     4261 cggcagcgcg ctcattgcgc cttgatgccg gccttcctga cgagctcggc gttcgcccgg
     4321 atctcgccct ggagatagag gttgaagcgc cccggcgtgt agttcatggg ctcggcgccg
     4381 aggcgcgaca tcttctcgcg cacgtcgggc gactggagca ccttcacgga ctcgtcgtag
     4441 agccgcgcga cgacgtcgcg cttcatcttc gccgggccga acatgccgac ccagaagttg
     4501 tagtcggagt tgagcacgcc ggcctcgagc gtcgtcggca gatcgggcag cgccttggag
     4561 cgctccgagc tgcccaccgc gagcgcgagc actttgccgt ccttgatcat cggcagcgcg
     4621 gcgttcacgg gacagaagta gtagtcgacg cggccggcga tcacctcggt gagcgcctcg
     4681 ggcgtgccct tgaacggaat gttgacgacg tcgatgcccg cgccgaggcg gaagcgctcg
     4741 gcgttgagat gcgtggcgct gccgacgccg accgaggccg agttcatcgc gccgggcttc
     4801 ttcctggcgg cggcgaccag ctccttcacc gaatggatgc ccttcgacgg cgagatgacg
     4861 agcacgttgg gaagctggcc gagcggcacg atgcccatga gatcgcgctc cgtgtcgaac
     4921 ggcaggtcct tgtaggtcga gggcgttacc gtatatgaag acgagtgcac gagcagcgtg
     4981 tggccgtcgg gctcggcttt ggcgacgatc gcctcgccga tcgtgccgcc ggcgccggga
     5041 cggttctcga cgatcagcgg ctggccgagt cgcttcgaca ggccctccga gacggtgcgc
     5101 gccatgatgt cggtgccgct gcccggcgtg aacggcacga tgacgcggat cggcttcgtc
     5161 ggccacgctg cggtggcctg accccacgcc gtcgcgtgcg cgccaagcgc cacggcggcg
     5221 attgcgaccc atctccagct gtgcgccacg atacctcgct tgtgcattga ggacgtcaaa
     5281 cgatacactt tttccgcggt cggcatccgc ccggcccgct ttctccggac gatcggatgc
     5341 tgggctggct gctcaagtcg cgcaggaaca aaggagacgc cgccgcggcc ggcgcgcacg
     5401 tgagcgccgg gttgcagcat caccaggcgg gccgtttgcg cgatgcggaa cgcgagtacg
     5461 ccgaggccct gggcctcgac ccggagtgca tcgacgcgct gcatttctcc ggcgtgatcg
     5521 cctaccagca ggagcgccac gacgacgcgc agcgcctgat ctcgcgcgcg ctctcgctcg
     5581 acgccgccaa tccgcccgcg cacaacaacc tcggcaacgt gctcctgaag caggggcggc
     5641 tcgaggatgc gctcgcgagc ttcgagcgcg cgatcgcgct ccagccggac tatctcgacg
     5701 cgcacgtcaa cctcgcgagc gtgtggctcg agcgcgcccg gccggagcgc gcgctggaat
     5761 gcatcggacg cgccctgcgc tcgctgcccg gggcggcgcg gctgcggctg cagcgcgcgc
     5821 aggcgctcgc cgccctcggc cgccacgagg aagcggccgc cgcgttccgc gaagcgatcg
     5881 aggccgatcc ggcttccgcg gcggcgtact ccgggctcgg gcatgccctg ggtcacgcgg
     5941 gacgcccgga cgaggccatc gcggcctatc gccgtgccgc cgcgctcgat cccgggggcg
     6001 cgggaccgcg ctacgacgcc ggcgtcgtct gccacgggat cggccggacc gatgaagcgc
     6061 tcgagtgctt cggcgaggcg ctcgccgtcg atccggagtt tgcgccggcg cgctgggcgc
     6121 tcaccaccgg gcagctgccc gccgtctacg cggacgcggc ggagccggag ctacggcgcg
     6181 ccgccttcgc gcgcgagctc gcgacgctcg agcgctgggt cgcgccggag cggctcgcgg
     6241 cgtccgtgca cgcggtgggc gcgcagcagc cgttctacct cgcctaccag gaccggaaca
     6301 accgcgagct gctcgggcgc tacggcgcgc tctgcgcgcg cgtgatggac gcctggctgc
     6361 gcgaagagcg cccggcgagc gccgcccggg cggcgcgcga tcgcgtccgc gtcggcgtcg
     6421 tctcggcgca tttccacaat cactcggtgt ggcacgcgat catcaagggc tggctcctgc
     6481 agatcgaccc ggaccgcttc gagctcgacg tcttccatct cggctcgacg cacgacgcgg
     6541 agaccgatct cgcgcgctcg cgcgctgcgg cctttcacgc ggggccgctg acgctgcggc
     6601 aatggaccga ggcgatcctc gagcggcgcc ccgacgtgct cctctatccc gacgtcggga
     6661 tggagcccgt caccgtgaag ctcgcgagcc tgcgcctggc gccggtacag gccgcggctt
     6721 ggggccatcc cgagaccacg gggctgccga ccgtcgactg ctatctctcg ggactcgaca
     6781 tggagccggc cggcgcggac gcgcattaca cggagcgcct cgtcgcgctg ccgcacctcg
     6841 gatgctggta cgaggcgacc ggggtcgagc ccgaggcgcc gccggtcgcg gtcgatgtcc
     6901 cgccgggcgc gccgctgctc gtgtgtcccg gggtgccgtt caagtacgcg ccggagcacg
     6961 atgcggcgct cgccggcatc gcgcgcgcgc tcggtcgctg ccgcttcgtc ttcttcacgc
     7021 atcgcccgcc cgagctctcc gagcggctgc gcctgcggct cgaggcggcg ttccggcgcg
     7081 aaggcctcga cttccgcgat ttcggcgtct tcgcgccctg gctcccccgc ggcgcgtttc
     7141 tcggcatgct gaaggcggcc gacgccatgc tcgatacgat cggcttttcc gggttcaaca
     7201 ccgcgatgca ggcggtcgaa gcggggttgc ccatcgtcac ggtggaaggc cgcttcatgc
     7261 gcggccggtt cgcgagtgcg atcctgcggc gcatggagat ctccgagctc gtcgcccgct
     7321 cggccgacga atacgtcgcc gtcgcgacga agctcggccg cgacgccgcg taccggcgcg
     7381 ggctgcgcga gcgcatcgag gcccggcgcg ggatcctgtt cggcgaccgg gcgccgatcc
     7441 tcgccctgga agcgttcctc gcggaggcgg cgggccgtta gcgacccggc cagttgtgta
     7501 tcatgcgcgc ttggctcgag gccatgcagt gaaagccgct cccttcgaat actcccgcgc
     7561 cgcatcgatc gccgaagcct gcgacctgct cgcccgccac ggcggcgacg cgaagctgct
     7621 cgccggcggc cagagcctcg tgccgatgat ggcgatgcgg ctgctgcgcc ccgccgcgct
     7681 gatcgacatc aacgagatcg cggcgctcaa gggcgtcgcg cgggacgagc gtttcgcgcg
     7741 catcggcgct tgcacgcgcc aggtcgcagt cgagcgcgac gacgcgctcg cggcgagcgt
     7801 gccgctgctg cgccgggcgc tcgcctgggt cgggcatgtg cagacgcgca accgcggcac
     7861 ggtcggcggc agcctcgcgc acgccgatcc gtcggccgag ctgccgctcg cggcgcaggt
     7921 gctgggagcg cagatcgcgc tgcgctcgaa gtccgggacg cgcacgctcg cggccgacgc
     7981 gttcttcgcg gggccgatga tgaccgcggc gcgtcccgac gaatgcatcg aggagatccg
     8041 ctggccggtg tggcccgagc ggcgcacggg atgcgcgttc accgaggtga gcgtgcgcca
     8101 cggcgacttc gcgatcgtcg cggcggcggc gcaggtcgca ctggacgccg acgggcgctg
     8161 cgcgcgcgcg tccttcggcc tcggcggcgc gggtggcacg ccgctcgcgt tcccgaagat
     8221 cgcggcgctc ctggtcggca cgaagctcga ggacgacgcg ctcgaggacg ccgcgaaggc
     8281 cgcggccgcc gggtgcgatc ccggcacgga cctgcacgcc accgcggact accggcggca
     8341 cctcgcgcgc gtgctcgtcg cgcgcgtcct gcgcgaggcc cgcgacgggg cgaaggcgcg
     8401 atgagcgaga ccctgcattc catctccgtc gaggtgaacg gcgtgcgccg cgaggctcgc
     8461 gtgccggcgc gcctgtcgct cgtcgactgg ctgcgcgacg agctgcgcct caccggcacg
     8521 cacgtcggct gcgagcacgg cgtgtgcggc gcgtgcacgg tgctggtcga cggcaccagc
     8581 atccgatcct gtttgatgtt cgcagttcag gcgaatggtc gatccgtaat gacggtggag
     8641 gcgctt
//
LOCUS       DATDMW010000112         3636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_653029, whole genome shotgun sequence.
ACCESSION   DATDMW010000112 DATDMW010000000
VERSION     DATDMW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653029"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VI319_05040"
     CDS             <1..843
                     /locus_tag="VI319_05040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6863245.1"
                     /translation="WTRLGRFDHAAPLGVVLRELFPRKKLAAPEGRLQILIAGCGTGA
                     HPARSALRFSDAAVVAMDLSRASLAHAVRKTRDLGITNVEYLQGDLLALPRLGRKFGL
                     IECMGVLHHLRDPMQGWRALLEVLAPGGYLRIGLYSELGRTHIVRAQRFAAERGHGST
                     DAGIRALRQDLMRAAPADASLARVARIRDFYSISGTRDLLLHVQEQRYTLARLAQTLA
                     ELGLEFLGFELPGTDSARRYRLRYPADLDMDDLASWQAFEESEPETFTGMYQFWARRA
                     RTGR"
     gene            complement(889..1251)
                     /gene="erpA"
                     /locus_tag="VI319_05045"
     CDS             complement(889..1251)
                     /gene="erpA"
                     /locus_tag="VI319_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY6863246.1"
                     /translation="MNAVTDSVTEMPAPLIFTDSAANKVKQLIDEEGNTELKLRVFVT
                     GGGCSGFQYGFTFDEVKNDDDTVMEKNGVMLLIDPMSYQYLVGAEIDYTEGLEGAQFV
                     IKNPNATSTCGCGSSFSA"
     gene            complement(1327..1740)
                     /locus_tag="VI319_05050"
     CDS             complement(1327..1740)
                     /locus_tag="VI319_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6863247.1"
                     /translation="MFKKQPTKPQNRIDSLIGAGTRIEGNVTFSGGLRVDGEVIGNVA
                     AVPDQPSTLVVSEQARIDGEITVSHLVVNGTINGPVHASEFLELQSRCRVKGDVQYNV
                     LEMHLGAIVEGRLAHASAESKPVELKLAASKPSGA"
     gene            complement(1753..2481)
                     /locus_tag="VI319_05055"
     CDS             complement(1753..2481)
                     /locus_tag="VI319_05055"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEY6863248.1"
                     /translation="MQLRFGISAPKMTVRTHLAWYWRWLGTMVMIAVSLALAAWMYDA
                     GRRFAGFDRTELELEVSKLKDSLSDVKSEAARLRSIATASESRLKIEQAAQQQLAGQV
                     KALEDENNRLREDLAFFDTLIPAERRGDKVSILGFKVDRDVLPGEFRYRLLVLQGGKR
                     DRPFSGSLQLIVEVQRDGKNDMILMPNPADAGSAAFKLSFKYFHRVEGTFRVPAEVKV
                     RAVQVRVMENGSGDARAVQNAVIS"
     gene            complement(2512..3540)
                     /gene="argC"
                     /locus_tag="VI319_05060"
     CDS             complement(2512..3540)
                     /gene="argC"
                     /locus_tag="VI319_05060"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY6863249.1"
                     /translation="MLKVGIVGGTGYTGVELLRILARHPETELVAITSRKEAGIPVAR
                     FFPSLRGAVMLEFTDPAAAKLERCDIVFFATPNTVAMSQAKVLVDAGVRVVDLSADFR
                     IRDIAEWERWYKTKHAAPELVARAVYGLPEVHRRQIREARLVANPGCYPTAVQLGFLP
                     LVESGCIDLGHLVADAKSGVSGAGRKAEEHLLFAEATDNFKAYGLAGHRHWPEIRQGL
                     DQIAGTKIGLTFVPHLTPMIRGIHATLYARVTKEADFQKLFEQRYRDEAFVDVMPPGS
                     EPDTRSVRAANVCRIAVHRPNGGDMLVVLSVIDNLVKGAAGQAVQNMNIMFGLPEALG
                     LEQLPVVP"
BASE COUNT          563 a         1240 c         1222 g          611 t
ORIGIN      
        1 tggacccgcc tcgggcgctt cgatcacgcc gcgccgctgg gcgtggtcct gagggagctg
       61 ttcccccgca agaagctcgc ggcccccgag ggtcggctgc agatcctgat tgccggctgc
      121 ggcaccggcg cgcacccggc gcgctcggcg ctccggttca gcgacgcggc cgtggtcgcg
      181 atggacctca gccgagcgag cctcgcccac gcggtgcgca agacgcgcga tctcggcatc
      241 acgaacgtcg aatatctgca gggcgacctg cttgcgctgc cgcgcctggg ccgcaagttc
      301 ggcctgatcg aatgcatggg cgtgttgcat cacctgcgcg atccgatgca gggctggcgc
      361 gcgctgctcg aggtgctcgc gcccggcggg tacctgcgca ttggcctcta cagcgagctc
      421 gggcgcacgc acatcgtgcg cgcgcagcgc tttgccgcgg agcgcggcca tggctcgacc
      481 gacgcgggga tccgcgcgct acgccaggac ctgatgcggg cggcgcccgc ggacgcgagc
      541 ctcgccagag tcgcccggat acgcgatttc tacagcatca gcggcacccg agacctgctc
      601 ctgcacgtgc aggagcagcg ttatacgctc gcgcggctcg cgcagacgct cgccgagctg
      661 ggtctcgagt tcctgggctt cgagctcccc ggtacggaca gcgcgcgccg gtaccggcta
      721 cgctaccccg cggacctcga tatggacgac ctggcgagct ggcaggcctt cgaggagtcg
      781 gaacccgaga ccttcacggg catgtaccag ttctgggcgc gacgcgcccg gacgggcagg
      841 taaacggaaa cgggggccgt cggcccccgc tgcaaagaag cgacgcgatc aggccgagaa
      901 cgaggatccg cagccgcagg tgctggtcgc gttcgggttt ttgatcacga actgcgcacc
      961 ttccagtccc tcggtgtagt cgatctcggc gccgacgagg tactggtagc tcatcgggtc
     1021 gatgagcagc atgacgccgt tcttctccat cacggtgtcg tcgtcgttct tcacctcgtc
     1081 gaaggtgaag ccgtactgga agcccgagca gcccccgccg gtgacgaaga cccggagctt
     1141 gagctcggtg ttgccttcct cgtcgatcag ctgcttcacc ttgttggcgg cgctgtcggt
     1201 gaagatcagc ggggccggca tttcggtaac tgagtcggtc actgcgttca tggtctgtct
     1261 cctgccggca ttatcggccg caccggcgga cgggtcaaat ggaggtgggg gcgtgcgccg
     1321 tggagttcaa gcgccgctgg gcttgctggc ggcgagcttg agctcgacgg gcttcgactc
     1381 ggcggaggca tgcgccaaac ggccttcgac gatcgcgccg agatgcatct ccagcacgtt
     1441 gtactgcacg tcgcccttca cccggcagcg cgattgcagc tcgagaaact cgctcgcgtg
     1501 cacgggcccg ttgatggtgc cgttgaccac cagatgcgac acggtgatct cgccatcgat
     1561 gcgcgcctgc tcgctcacga ccagcgtcga gggctggtcg gggaccgccg cgacgttgcc
     1621 gatgacttcg ccgtcgacgc gcaggccgcc ggagaacgtg acgttcccct cgatgcgcgt
     1681 gccggcgccg atcaggctgt cgatccgatt ctgcggtttt gtcggctgct tcttgaacat
     1741 ggttctctcc gcttacgaaa tcaccgcgtt ctggaccgcg cgtgcgtcgc ccgatccgtt
     1801 ctccatgacg cgcacctgca cggcccgaac cttcacttcg gccggtaccc ggaacgtgcc
     1861 ctcgacgcgg tggaaatact tgaaggaaag cttgaacgcc gcgctgcccg cgtcggccgg
     1921 attcggcata agaatcatat cattcttgcc gtcccgctgc acctcgacga tgagctgcag
     1981 cgagcccgag aaaggccggt cgcgcttgcc cccctggagc acgagcagcc ggtagcggaa
     2041 ttcgcccggg agcacgtcgc ggtcgacctt gaacccgagg atgctcactt tgtcgccgcg
     2101 gcgctccgcc gggatcaacg tgtcgaagaa cgcgaggtcc tcgcgcagcc ggttgttctc
     2161 gtcctcgagc gccttcacct ggccggcgag ctgctgctgc gccgcttgct cgatcttgag
     2221 gcgcgattcg ctcgcggtcg cgatcgagcg caggcgcgcc gcctcgctct tcacgtcgga
     2281 caaggaatcc ttcagcttcg agacctcgag ctcgagctcg gtgcggtcga agcccgcgaa
     2341 gcgccggccc gcgtcgtaca tccaggcggc gagggcaagc gataccgcga tcatgaccat
     2401 cgtgccgagc cagcgccagt accaggcgag gtgcgtgcgc accgtcatct tcggcgcgga
     2461 gatgccgaac cgaagctgca ttcgtttcca gagcgagggc accgcgcggc ctcaggggac
     2521 gaccggcagc tgctcgaggc cgagcgcctc gggcaacccg aacatgatgt tcatgttctg
     2581 gaccgcctgt ccggcggcgc ctttcacgag gttgtcgatc accgagagca cgaccagcat
     2641 gtcgccgccg ttcggacggt gcacggcgat gcggcacacg ttggccgcgc gcaccgagcg
     2701 cgtgtcgggc tcgctgcccg gcggcatcac gtcgacgaac gcctcgtcgc ggtagcgctg
     2761 ctcgaagagc ttctggaagt cggcctcctt cgtcacgcgc gcgtagaggg tcgcgtggat
     2821 gccgcggatc atgggcgtca ggtgcgggac gaatgtgagc ccgatcttcg tcccggcgat
     2881 ctggtcgagg ccctggcgga tctccggcca gtggcggtga ccggcgagcc cgtacgcctt
     2941 gaaattatcg gtcgcctcgg cgaacagcag gtgctcctcc gccttgcgcc ctgcgccgct
     3001 cacgccggac ttcgcgtcgg cgaccaggtg gccgagatcg atgcaaccgg actcgaccag
     3061 gggaaggaat ccgagctgca ccgcggtcgg atagcagccg ggattcgcga cgaggcgcgc
     3121 ttccctgatc tggcgacgat gcacttcggg cagaccgtag accgcacgcg cgacgagctc
     3181 gggcgccgca tgcttcgtct tgtaccagcg ctcccattcc gcgatgtcgc ggatgcggaa
     3241 atccgccgac agatcgacga cccgcacgcc agcgtcgacc aggaccttgg cctgcgacat
     3301 cgcgacggtg ttcggcgtcg cgaagaagac gatgtcgcag cgctcgagct tcgccgccgc
     3361 cggatcggtg aactcgagca tcaccgcgcc gcgcaggctc gggaagaagc gcgcgaccgg
     3421 gatgcccgct tccttgcgcg aggtgatggc gacgagctcg gtctcggggt ggcgcgcgag
     3481 gatgcgcagc aactccactc cggtatagcc cgtaccgccg acgatcccga ccttcagcat
     3541 ccttgcccct gtagcgcggc gcgcccggcg ttcattgtac ccacagccgc aagcacccta
     3601 gcgggaaact ctcacaccgc ccgccgcggc gggggg
//
LOCUS       DATDMW010000113         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_653111, whole genome shotgun sequence.
ACCESSION   DATDMW010000113 DATDMW010000000
VERSION     DATDMW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653111"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VI319_05065"
     CDS             <1..529
                     /locus_tag="VI319_05065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY6863250.1"
                     /translation="ASRRYPAEMFARALELLARDTGCHLVLTGDAHEAALVESIRARM
                     SAPSHSLAGRLDLGGLGAVIGAADVLVSNNTGPVHIAAALGTPVVDLYALTNPQHTPW
                     KVPCRVLFHDVPCRYCYKSMCPSGHHECLRNVAPETVAEAARQLLDGRGAHFPELAGG
                     AVPEGCSVIRPAVVE"
     gene            532..>2064
                     /locus_tag="VI319_05070"
     CDS             532..>2064
                     /locus_tag="VI319_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6863251.1"
                     /translation="MYTLGINAVYHDSAACLVRDGEVVAAAEDERFTRIKHGKRPVPF
                     TTWELPYHAIDYCLAAAGIELAEVDHVAYSYDPFLLLGQYKHDAAISLPMEPSAHAAS
                     VEGASPWEPLALAYVVNAERQLVDGAPHHLRARFASAKTDRRWRWHFVEHHGAHEASA
                     YLAAPFDECAVMTLDGRGERATTSYGHFDGERYRRIGEVEVPHSLGLLYEQVTDYLGF
                     LHSSDEYKVMALASYGKPAYLGEFREMLEMRDGGDYVLHPPRLAERFGPPRRSGAELL
                     PKHLDLARSLQALLEETAVGLARWLRRETGAEYLAMAGGVALNCVMNARIRDARLFRG
                     VWVQPAAGDAGTALGAALVVDRQARGGGARRWRMDHAYLGPEYGEAEIRAFLERAKLS
                     YRRLSDIAGETAGILAEDRIVGWFQGRMEFGPRALGARSILASPLAADMQARLNEIKD
                     REDFRPVAPAVVEEEAPAWFERARVSPFMLFVHDVVPDKADRIPAVRHVDGTARIQTV
                     SRS"
BASE COUNT          274 a          762 c          737 g          291 t
ORIGIN      
        1 ggcctcgcgg cgctatccgg ccgagatgtt cgcgcgcgcg ctcgagctgc tcgcgcgcga
       61 taccggctgc catctcgtgc tgacgggcga cgcgcacgag gccgcgctcg tcgagtccat
      121 ccgcgcgcgg atgtcggcgc cgtcgcactc gctcgcggga cgcctcgacc tcggcggcct
      181 gggcgcggtg atcggcgcgg ccgacgtgct ggtgtcgaac aacaccgggc ccgtgcacat
      241 cgccgcggcg ctcggcacgc cggtcgtcga tctgtacgcg ctcaccaacc cgcagcacac
      301 gccgtggaag gtcccttgcc gcgtgctgtt ccacgacgtg ccctgccgct actgctacaa
      361 gagcatgtgc ccgagcgggc atcacgagtg cctgcgcaac gtcgcgcccg agaccgtcgc
      421 cgaggccgcg cgccagctgc tcgacgggcg cggcgcgcat ttccccgagc tcgcgggggg
      481 cgcggtgccc gaggggtgct ccgtcatccg ccccgcggtc gtggagtagc gatgtatacg
      541 ctcggcatca acgccgtcta ccacgacagc gcggcgtgcc tggtgcgcga cggcgaggtc
      601 gtcgccgcgg ccgaggacga gcgcttcacc cgcatcaagc acggcaagcg cccggtgccc
      661 ttcacgactt gggagctgcc ctaccacgcg atcgactact gcctggcggc ggccggcatc
      721 gagctcgccg aggtggacca cgtcgcctac tcgtacgatc ctttcctgct gctcgggcaa
      781 tacaagcacg atgcggcgat ctcgctgccg atggagccca gcgctcatgc cgcctcggtc
      841 gagggcgcgt cgccctggga gccgctcgcg ctcgcctacg tcgtgaacgc cgagcgccag
      901 ctggtcgacg gcgcgccgca ccacctgcgc gcgcgcttcg cgtcggcgaa gaccgaccgg
      961 cgctggcgct ggcacttcgt cgagcaccac ggcgcgcacg aggcgagcgc gtacctcgcg
     1021 gcgccgttcg acgagtgcgc agtgatgacg ctcgacggcc gcggcgagcg cgcgaccacg
     1081 agctacggcc acttcgacgg cgagcgctac cgccgcatcg gcgaggtgga ggtgccgcat
     1141 tcgctcgggc tgctctacga gcaggtgacc gactacctcg gcttcctgca ttcgtcggac
     1201 gagtacaagg tgatggcgct cgcctcatac ggcaagccgg cgtacctcgg cgagttccgg
     1261 gagatgctgg agatgagaga cggcggcgac tacgtgctgc acccgccgcg cctcgccgaa
     1321 cgcttcggtc cgccccgcag gagcggcgcc gagctgctcc cgaagcacct cgacctcgcg
     1381 cgctcgctgc aggcgctgct cgaggagacc gcggtcgggc tcgcgcgctg gctgcgccgc
     1441 gagacgggcg ccgaatacct ggcgatggcg ggcggcgtcg cgctgaactg cgtcatgaac
     1501 gcgcgcatcc gtgatgcccg cctttttcgc ggcgtgtggg tgcagcccgc cgcgggcgac
     1561 gccggcacgg cgctgggcgc ggcgctcgtc gtcgaccgcc aggcgcgcgg cggcggcgcg
     1621 cggcgctggc gcatggacca cgcctacctc ggacccgagt acggcgaggc cgagatccgg
     1681 gcgttcctcg agcgcgcgaa gctgagctac cgccgcctct ccgatatcgc cggggagacc
     1741 gccggcatcc tcgccgagga caggatcgtc ggctggttcc agggccgcat ggagttcggc
     1801 ccgcgcgcgc tcggcgcgcg ctcgatcctc gcctcgccgc tcgcggccga catgcaggcg
     1861 cggctgaacg agatcaagga ccgcgaggat ttccggccgg tcgcgccggc cgtcgtcgag
     1921 gaggaggcgc ccgcatggtt cgagcgtgcg cgcgtgtcgc ccttcatgct gttcgtgcac
     1981 gacgtcgtcc cggacaaggc ggaccgcatc ccggccgtgc gccatgtcga cggcaccgcg
     2041 cgcatccaga ccgtaagccg ctcg
//
LOCUS       DATDMW010000114         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_653710, whole genome shotgun sequence.
ACCESSION   DATDMW010000114 DATDMW010000000
VERSION     DATDMW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653710"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VI319_05075"
     CDS             <1..355
                     /locus_tag="VI319_05075"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEY6863252.1"
                     /translation="SPDLPFEVRAFAVTVERRELHERIARRFDAMLEAGLLDEVRELR
                     RTYRLTARTPSMRAVGYRQAWEHLEGETTLGEMRERAIAATRQLAKRQITWLRSFPEV
                     LPIPPDPERIAHSFL"
     gene            complement(340..567)
                     /locus_tag="VI319_05080"
     CDS             complement(340..567)
                     /locus_tag="VI319_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863253.1"
                     /translation="MDMLDLVQWPAMAVTVLAAWLVGSRSAARRAAGFWCFLAGNVLW
                     VVWGFHDHAYALIFLQFCLAAMNARGVYKNE"
     gene            614..1528
                     /gene="gnd"
                     /locus_tag="VI319_05085"
     CDS             614..1528
                     /gene="gnd"
                     /locus_tag="VI319_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY6863254.1"
                     /translation="MQIGIVGLGRMGAGMARRLARKGVRVVCYDQAEAARARLGGNGG
                     NLECVESLAALCARLDGVRVILLMLPAGSAVEETIAALVPCVSADDTLVDGGNSFYRD
                     SMRRALALSQQGLRYIDAGVSGGVHGLENGYCLMLGGTPKSIEMFEPFARLLAPEPEK
                     GWLHCGPSGAGHYAKMIHNGIEYGMMQALAEGFSLLAARKELDIDVQRLAETWRHGSV
                     VRSWLLDLCSEILKGNETLSGVAPVVADSGEGRWTALESVQLGVPAPVITAALISRFS
                     SQGASDYADRLLAMMRNQFGGHAVEKEK"
     gene            1587..2063
                     /locus_tag="VI319_05090"
     CDS             1587..2063
                     /locus_tag="VI319_05090"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY6863255.1"
                     /translation="MIVVVMGVSGAGKTTIGRRLAERLGLPFIEGDDYHPPANVAKMA
                     SGVPLADEDRWPWLDALNAALRRQRNAVLACSALKAAYRARLLAGIEDARVVHLYGDK
                     AIIAGRLKQRAHRYMPASLLDSQFAILEPPAGAIAIDVSGDVERTVEEIARRVSVL"
     gene            complement(2050..>2692)
                     /locus_tag="VI319_05095"
     CDS             complement(2050..>2692)
                     /locus_tag="VI319_05095"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY6863256.1"
                     /translation="FTVIPVEKLIGPGYLAKRAKLIGETAMRRAPPGDREGGTSHLSI
                     VAPDGEAVAMTTTIESSFGSRIMVRGFLLNNELTDFDFLPGGPNQPGPRKRPRSSMAP
                     TLVFRGGELQLVAGSPGGSAIIDYVAKTLVARFDWHLDLQAAIEVPNFGSRTGALEIE
                     AGSAFAPLAKALAARGHTVELLPLQSGVYGIERVPGGWRGGADPRREGVARGR"
BASE COUNT          414 a          933 c          960 g          385 t
ORIGIN      
        1 ctcgcccgac cttcccttcg aggtgagagc gttcgcggtg accgtcgaaa ggcgcgagct
       61 gcacgagcgg atcgcgcggc gcttcgacgc catgctcgag gcgggattgc tggacgaggt
      121 gcgcgagctg cgaagaacgt atcgcctgac cgcgcgcacg ccgagcatgc gcgcggtcgg
      181 ctatcgccag gcgtgggagc acctcgaggg cgagaccacg ctcggcgaga tgcgcgagcg
      241 ggcgatcgcg gccacgcgcc agctcgccaa gcgccagatc acctggctgc gctcgtttcc
      301 ggaggtgctg ccgatcccgc ccgacccgga gcgcatcgct cactcgttct tgtagacgcc
      361 gcgcgcgttc atcgcggcga ggcagaactg caggaagatc agcgcatacg cgtggtcgtg
      421 gaaaccccac accacccaga gaacgttgcc ggcgaggaag caccagaagc cggcggcgcg
      481 gcgcgcggcg gagcgcgagc cgacgagcca tgcggcgaga acggtgaccg ccatcgccgg
      541 ccactgcacc agatcgagca tatccatcgt cacgctcaga ccctagaatc ggtcgcttcg
      601 ttcaggagat ggtatgcaga taggcatagt cggcctcggg cgcatgggcg ccggcatggc
      661 gcggcgcctc gcgcgcaagg gcgtgcgcgt cgtgtgctac gaccaggccg aagcggcgcg
      721 ggcgaggctc ggcggcaacg gcgggaacct ggagtgcgtg gagagcctcg ccgcgctttg
      781 cgcgaggctg gacggcgtgc gcgtgatcct gctgatgctg cccgccggca gcgcggtcga
      841 ggagacgatc gccgcgctcg tgccgtgcgt ctccgccgac gacaccctcg tcgacggcgg
      901 caacagcttc taccgcgact cgatgcgccg cgcgctcgcg ctctcgcagc aggggctgcg
      961 ctacatcgac gcgggcgtgt cgggcggcgt gcacggcctc gagaacggct attgcctgat
     1021 gctcggcggg acgcccaagt cgatcgagat gttcgagccc ttcgcgcgcc tgctcgcgcc
     1081 ggagccggag aagggctggc tgcactgcgg gccgagcggc gccgggcact acgcgaagat
     1141 gatccacaac ggcatcgagt acgggatgat gcaggccctc gccgagggct tctccctcct
     1201 cgccgcgcgc aaggagctcg acatcgacgt gcagcgcctc gccgagacgt ggcgccacgg
     1261 cagcgtggtg cgctcctggc tgctcgacct gtgcagcgag atcctgaaag gcaatgaaac
     1321 gctttccggc gtcgcgccgg tggtcgcgga ctccggcgaa gggcgctgga ccgcgctcga
     1381 gtcggtgcag ctcggcgtgc ccgcgcccgt gatcaccgcg gcgctgatca gccgcttctc
     1441 cagccagggc gcttcggatt acgcggaccg gctcctcgca atgatgcgca accagttcgg
     1501 cgggcacgcc gtcgagaagg aaaagtgaat gacggggtca ggtcttgcct tgatgcatcg
     1561 gatgcgcgaa ttcaagacct gaccccatga tcgtcgtcgt gatgggcgtt tcgggggcag
     1621 ggaagacgac gatcggccgc cggctcgccg agcgcctcgg cctgcctttc atcgaaggcg
     1681 acgactacca tccgccggcg aacgtggcca agatggcgag cggcgtgccg ctcgcggacg
     1741 aagaccgctg gccgtggctc gacgcgctga acgcggcgct caggaggcaa cgcaacgccg
     1801 tgctcgcctg ctcggccctg aaggccgcct accgcgcgcg cctgctcgcc ggcatcgaag
     1861 acgcccgggt ggtccatctg tacggcgaca aggcgatcat cgccggccgg ctgaagcagc
     1921 gcgcgcaccg ctacatgccc gcctcgctcc tcgacagcca gttcgccatt ctcgagccgc
     1981 ccgccggcgc aatcgcgatc gacgtgtcgg gcgatgtcga gcgcacggtc gaagagatcg
     2041 cgcgccgggt cagcgtcctc tagcgacccc ctcgcgccgc ggatcggcgc cgccgcgcca
     2101 gccgccgggg acgcgctcga tgccgtacac cccgctctgc agcggcagca gctcgacggt
     2161 atgcccgcgc gccgcgagcg ccttcgcgag gggcgcgaac gcgctgcccg cctcgatctc
     2221 cagcgcgccg gtgcggctgc cgaagttggg cacctcgatc gccgcctgga ggtcgaggtg
     2281 ccagtcgaag cgcgcgacga gcgtcttcgc cacgtagtcg atgatcgccg agccgcccgg
     2341 cgagccggcg acgagctgga gctcgccgcc gcggaagacg agcgtcggcg ccatgctgct
     2401 cctcggccgc ttgcgcggcc ccggctggtt cggcccgccc ggcaggaaat cgaagtcggt
     2461 gagctcgttg ttgaggagga agccgcgcac catgatgcgg ctgccgaacg agctctcgat
     2521 ggtcgtggtc atcgccaccg cctcgccgtc cggcgcgacg atcgagagat ggctcgtccc
     2581 gccctcgcgg tcgcccggcg gggcgcggcg catcgccgtt tcgccgatga gctttgcccg
     2641 cttcgcgaga tagcccgggc cgatcagttt ttcgacgggt atgacggtaa ag
//
LOCUS       DATDMW010000115         5369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1401679, whole genome shotgun sequence.
ACCESSION   DATDMW010000115 DATDMW010000000
VERSION     DATDMW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401679"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VI319_05100"
     CDS             <1..890
                     /locus_tag="VI319_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6863257.1"
                     /translation="PTTELVSTTRKVTSVVPEGAVWVDIGFGDLELSSWAMDYQRLMH
                     AHLRVMADTTVAIPALTGLVKRRIAGDGALKKTIDERTKRWHELHRKTRAQWAAEAKE
                     DWDASPIPLGRLASEVWDAIKGEDWVLTANTLEEWSLKLWNFDKPYRHPGKSLGTSTQ
                     IGISLGVALAHRDAGRLVVDLQPDGDLMFDAGALWVAAKHKIPMLVVMYNNRAYYNDW
                     EHQIRMARHRGTPLDRAHIGMDIEGPFVDFAGLARSMGWYAEGPIERGQDVAAALKRA
                     IAKVKAGQPALVDTLTQKR"
     gene            887..1861
                     /locus_tag="VI319_05105"
     CDS             887..1861
                     /locus_tag="VI319_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6863258.1"
                     /translation="MMRALALVAALLFANAAQSADVAAAKREGTLTVYASMAEKDLLR
                     LVSAFEKRYGIKVNVWRSGKNKVLQRAVTEARAGRFEVDFVHNPSPEMELLHREGLLA
                     PVRSPYQDALIPEAVARHHEWAGPRVYIFVQAYNTQKVRRDELPKTFEDLLDPRWKGR
                     LGIEGKEQEWFFTLVHAMGEERGLRFFHDLVAMNGLSVRSGNALLNNMVVAGEVPLAV
                     TMYSYLAEQSKRAGAPIDWIALKPTIAYTDGIGIMKHAPHPNAALLFYEFVLSDGQQL
                     LAGLNHVISSRKNEAYIRSFDPTFIDVEAVLADYDRWTKVFEDTIYAR"
     gene            1858..3126
                     /locus_tag="VI319_05110"
     CDS             1858..3126
                     /locus_tag="VI319_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6863259.1"
                     /translation="MTTREQNETLTKTGRGTPLGELLRRYWLPVLLASELPEADCPPV
                     RAKLLGERLVAFRDTGGRIGLVGEFCAHRGVSLWFGRNEEHGIRCPYHGWKYDVTGQC
                     VDVPSEPADNGFCAKIKLDAYPCIERAGVIWAYLGPKEARPPEPAYDWTLVPDSHRYI
                     SKRLQECNWLQAMEGALDSIHSTFLHRYSVGEDPLLKRDPASLALIKGDTSPKFVPTV
                     SPAGLYVATRRDAGPDAYYWRVTQWLMPCFSFFPPYGDNPHGGHAFVPIDDERCWVFS
                     IDYQPRRPLSAEERDAAHAGKGIHVRLIEGTFTPVANKRNDYLVDREAQKAKKTFSGV
                     FDVGVQDAAIQESMGPVQDRAREHLVSSDNGIIMTRKRLTDAAAAVARGEPPPGLDPA
                     AQGERAKSMVAPRDVPLLEAMNSRKATEAA"
     gene            3123..3941
                     /locus_tag="VI319_05115"
     CDS             3123..3941
                     /locus_tag="VI319_05115"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6863260.1"
                     /translation="MSGTIERVGVIGAGRMGQPIIGHLARKGFAVQVHDVDPAKKAAV
                     EKRGARWADLRTLARESQAILVCVGYDRELRELLSERGLLKDAARGTIVAVLSTVHPR
                     TIKELAQQAAPAGVDVVDTTVCRGGWAADEGTLLSFVGGPAQVVERLKPVIAAYSSDI
                     EHTGEAGTAQVAKAANNLMLWACLVASHEGLALAHRYGLDVEKLRKVLLTSSATSGAL
                     ENWGNQTMAWAEDDMAIVAEMASEAGIALPQAGVDREICRALKPRRYQLDKYGT"
     gene            3931..4935
                     /locus_tag="VI319_05120"
     CDS             3931..4935
                     /locus_tag="VI319_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6863261.1"
                     /translation="MERRRVLAALAGLIGNAAVAPSARGQDLLQRARREGTLTVYTSF
                     PPEDIAAVNALFEKRTGVKVRPWRSSSENVLQRTLAETRSGRFDVDVVECNSIPLETL
                     HREGVLQRVQTPGTADLVPAAVPKHGEWVASRLSLFVQAYNTRQVRKDELPKSYADLL
                     DPRWKGRLGVEAADMDWFSEVVRGLGEEKGLKLFRDLVATNGLSVRRGHSVIAQLVAT
                     GDIPLALTVYNFTAEQLKRRGAPLDWFVLPPAVAHANGVAIPKRAPRPHAALLYYEFM
                     IGEEAQRLLASRDSIPTSTKVESALDRSSFRVVDPARMLDEGERWAKVYDEIIVKGSR
                     "
     gene            4932..>5369
                     /locus_tag="VI319_05125"
     CDS             4932..>5369
                     /locus_tag="VI319_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863262.1"
                     /translation="MKRDPVDVVDKPEPVANESHYHSKKDRIFVRAIAGQYNLKDELK
                     RLRSLPRVRKAKDIRFNDGPQTFSRHYVEPKDGITQTLHIHLEEYSPGAISQKHGHVN
                     EAAFYILDGRGYEIHDGVRYDWQAGDVAIVHNNCVHQHHNADPE"
BASE COUNT          921 a         1814 c         1900 g          734 t
ORIGIN      
        1 ggccgacgac cgagctggtc agcacgacgc gcaaggtcac gagcgtcgtg cccgagggcg
       61 cggtctgggt cgacatcggc ttcggcgacc tcgagctctc gagctgggcg atggactatc
      121 agcgcctgat gcacgcgcat ctgcgcgtga tggccgacac cacggtcgcg atcccggcgt
      181 tgaccgggct cgtgaagcgc cggatcgccg gcgacggcgc gctgaagaag acgatcgacg
      241 agcgcacgaa gcgttggcac gagctgcacc ggaagacaag agcgcaatgg gccgcggaag
      301 cgaaagaaga ctgggacgcg agccccatcc cgctcgggcg cctcgcgtcc gaggtctggg
      361 acgcgatcaa aggcgaggac tgggtcctca ccgcgaacac gctcgaggag tggagcctca
      421 agctctggaa cttcgacaag ccttatcgcc acccgggcaa gtcgctcggg acgtcgaccc
      481 agatcgggat ctcgctgggc gtcgcgcttg cccaccgcga cgccggccgc ctggtcgtcg
      541 acctccagcc cgacggcgac ctgatgttcg acgcgggcgc gctctgggtg gcggcgaagc
      601 acaagatccc gatgctggtc gtcatgtaca acaaccgcgc ctactacaat gactgggagc
      661 accagatccg catggcgcgc catcgcggca cgcccctgga ccgcgcgcat atcggcatgg
      721 acatcgaggg tccgttcgtc gacttcgccg ggctcgcgcg ctcgatgggc tggtacgccg
      781 aaggcccgat cgagcgcggg caagacgtgg ccgcggcgct gaagcgcgcc atcgccaagg
      841 tcaaggcggg gcagccggcg ctggtcgaca ctctcacgca gaagcgatga tgcgagccct
      901 ggccctcgtc gccgccttgc tgttcgcgaa tgccgcgcaa tccgcggacg tcgcggcggc
      961 gaagcgcgag gggacgctga cggtctacgc ctcgatggcc gagaaggacc tgctgcgcct
     1021 cgtctcggcg ttcgagaagc gctacggcat caaggtcaac gtctggcgct cgggcaagaa
     1081 caaggtgctg cagcgcgcgg tgaccgaggc gcgcgcgggg cgcttcgagg tcgatttcgt
     1141 gcacaacccg tcgcccgaga tggagctgct gcaccgggaa ggcctgctcg cgcccgtgcg
     1201 ctcgccgtac caggacgcgc tcatccccga ggcggtcgcg cgccaccacg agtgggcggg
     1261 cccgcgcgtc tacatcttcg tgcaggcgta caacacgcag aaggtgaggc gcgacgagct
     1321 gccgaagacc ttcgaggacc tgctcgaccc gcgctggaaa ggccggctcg ggatcgaggg
     1381 caaggagcag gaatggttct tcacgctggt gcacgcgatg ggcgaggagc gcgggctgcg
     1441 cttctttcac gacctcgtgg cgatgaacgg gctctcggtc cgcagcggca acgcgctcct
     1501 gaacaacatg gtggtcgccg gcgaggtccc gctcgcggtg accatgtaca gctacctcgc
     1561 cgagcagtcg aagcgcgcgg gggcgccgat cgactggatc gcgctcaagc cgacgatcgc
     1621 gtacaccgac ggcatcggca tcatgaagca cgcgccgcac ccgaacgccg cgctgctgtt
     1681 ctacgagttc gtcctgtccg acgggcagca gctgctcgcc gggctgaacc acgtgatctc
     1741 gagccggaag aacgaggcct atatccgcag cttcgacccg acgttcatcg acgtcgaggc
     1801 ggtgctcgcc gattacgacc gctggacgaa agtgttcgaa gacaccattt acgcccgatg
     1861 acgacgcgcg agcaaaacga gacgctgacg aagaccggcc gcggcacccc gctgggcgag
     1921 ctgctgcgcc ggtactggct gcccgtgctg ctcgcgagcg agctgcccga ggctgactgc
     1981 ccgccggtgc gcgcgaagct gctcggcgag cgcctcgtcg cgttccgcga caccggcggc
     2041 cgcatcgggc tcgtcggcga gttctgcgcc caccgcggcg tgagcctgtg gttcgggcgg
     2101 aacgaagagc acggtatccg ctgcccgtat cacggctgga agtacgacgt gacggggcag
     2161 tgcgtcgacg tgccgtcgga gcccgcggac aacggcttct gcgccaagat caagctggac
     2221 gcgtacccgt gcatcgagcg cgcgggcgtg atctgggcgt acctcggtcc gaaggaggcg
     2281 aggccgcccg agcccgcgta cgactggacg ctcgtcccgg actcgcatcg ctacatctcg
     2341 aagcggctgc aggagtgcaa ctggctgcag gcgatggaag gcgcgctcga ctcgatccat
     2401 tcgacgttcc tgcaccgcta ctcggtcggc gaagatcctc tgctcaagcg cgatcccgcg
     2461 agcctggcgc tgatcaaggg cgataccagc ccgaagttcg tcccgaccgt ctcgcccgcc
     2521 ggactgtacg tcgcgacccg gcgcgacgcc gggcccgacg cgtattactg gcgcgtcacg
     2581 caatggctga tgccgtgctt cagcttcttc ccgccgtacg gggacaatcc gcacggcggg
     2641 cacgccttcg tgccgatcga cgacgagcgc tgctgggtct tcagcatcga ctaccagccg
     2701 agacgcccgc tctcggccga ggagcgcgac gccgcgcacg cgggcaaggg tatccacgtg
     2761 cggctgatcg aaggaacctt cacgccggtc gcgaacaagc gcaacgacta cctcgtcgac
     2821 cgcgaggcgc agaaggcgaa gaaaacattc tcgggcgtgt tcgacgtcgg cgtgcaggac
     2881 gcggccatcc aggagagcat ggggccggtg caggaccggg cgcgcgagca cctcgtgtcc
     2941 tcggacaacg gcatcatcat gacgcgcaag cgcctgaccg acgcggccgc ggcggtcgcg
     3001 cgcggcgagc cgccgccggg cctcgatccc gcggcgcagg gcgagcgcgc gaagtcgatg
     3061 gtcgcgccgc gcgacgtgcc gctgctcgag gcgatgaaca gccggaaggc gacggaggcg
     3121 gcatgagcgg aacgatcgag cgcgtcggcg tgatcggcgc gggccggatg gggcagccga
     3181 tcatcgggca cctcgcgcgc aagggattcg cggtgcaggt gcacgacgtc gacccggcga
     3241 agaaggcggc ggtcgagaag cgcggcgcgc gctgggccga tctccgcacg ctcgcgcgcg
     3301 aatcgcaggc gatcctggtg tgcgtcggct acgaccgcga gctgcgcgag ctgctctcgg
     3361 agcgcggcct gctcaaggac gcggcgcgcg ggacgatcgt cgcggtgctc tccaccgtcc
     3421 acccgcgcac gatcaaggag ctcgcgcagc aggcggcgcc cgcgggcgtc gacgtcgtcg
     3481 acaccacggt gtgccgcggc ggctgggcgg cggacgaggg cacgctgctc tcgttcgtcg
     3541 gcggtccggc gcaggtggtc gagcggctga agccggtcat cgccgcgtac tcgagcgaca
     3601 tcgagcacac gggcgaggcg ggcactgcgc aggtcgcgaa ggccgccaac aatctcatgc
     3661 tctgggcgtg cctggtcgcg tcgcacgagg gcctcgcgct cgcgcaccgc tatgggctcg
     3721 acgtcgagaa gctgcgcaag gtgcttctca ccagcagcgc caccagcggc gcgctcgaga
     3781 actggggcaa ccagaccatg gcctgggcgg aggacgacat ggcgatcgtc gccgagatgg
     3841 cgagcgaggc cggcatcgcg ctgccgcagg cgggcgtgga ccgcgagatc tgccgcgcgc
     3901 tcaagcccag gcgctaccag ctggacaagt atggaacgta ggcgcgttct cgcggcgctc
     3961 gcggggctga tcggaaatgc ggcggtcgcg ccgtctgcgc gcggccagga cctgctccag
     4021 cgcgcgcgcc gcgaggggac gctcacggtc tacacgtcgt tcccgcccga ggacatcgcg
     4081 gcggtcaacg cgctgttcga gaagcgcacc ggcgtcaagg tgaggccgtg gcgctcgtcg
     4141 tcggagaacg tcctgcagcg cacgctcgcc gagacgaggt ccggccgctt cgacgtcgac
     4201 gtcgtggagt gcaactcgat cccgctcgag acgctgcacc gcgagggcgt actgcagcgc
     4261 gtgcagaccc ctggcaccgc ggacctggtg ccggccgcgg tgccgaagca cggcgaatgg
     4321 gtcgcgtcgc gcctgagcct cttcgtgcag gcgtacaaca cccggcaggt ccgcaaggac
     4381 gagctcccga agagctatgc cgacctgctc gatccgcgct ggaagggccg cctcggcgtc
     4441 gaggcggccg acatggactg gttctccgag gtcgtgcgcg gcctcggcga ggagaagggc
     4501 ctcaagctct tccgcgacct cgtggcgacg aacgggctgt cggtgcgccg cgggcacagc
     4561 gtcatcgcgc agctggtcgc gaccggcgac atccccctcg cgctgacggt ctataatttc
     4621 actgcggaac agctcaagcg ccgcggcgcg ccgctcgact ggttcgtgct cccgcccgcg
     4681 gtcgcgcacg ccaacggcgt cgcgattccg aagcgggctc cgcgcccgca cgcggcgctg
     4741 ctgtactacg agttcatgat cggcgaggag gcgcagcgcc tgctggcgag ccgcgactcg
     4801 atcccgacca gcacgaaagt ggagagcgct ctcgaccgga gctcgttcag ggtcgtcgac
     4861 ccggcgcgca tgctcgacga aggcgagcgc tgggccaagg tgtacgacga gatcattgtg
     4921 aaaggaagcc gatgaaacgc gatcccgtgg acgtggtgga caagcccgag cccgtggcca
     4981 acgaatccca ttaccacagc aagaaggacc ggatcttcgt ccgggccatc gccgggcagt
     5041 acaacctcaa ggacgagctg aagcgcctgc gctcgctgcc gcgcgtgcgc aaggcgaagg
     5101 acatcaggtt caacgacggc ccgcagacct tcagcaggca ctacgtcgag cccaaggacg
     5161 ggatcacgca gacgctgcat atccacctgg aggagtactc gccgggcgcc atatcgcaaa
     5221 agcacggtca cgtgaacgag gcggcgttct acatcctcga cgggcgcggc tacgagatcc
     5281 acgacggcgt gcgctacgac tggcaggcgg gcgacgtcgc gatcgtgcac aacaattgcg
     5341 tgcaccagca ccacaacgcc gaccccgag
//
LOCUS       DATDMW010000116         8022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_157523, whole genome shotgun sequence.
ACCESSION   DATDMW010000116 DATDMW010000000
VERSION     DATDMW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157523"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..702)
                     /locus_tag="VI319_05130"
     CDS             complement(52..702)
                     /locus_tag="VI319_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY6863263.1"
                     /translation="MAVRAVLFDLDGTLADTASDLARALNSVRAERGLGPVPPAMTRP
                     HTSKGARGLLGVGMGVAPEHATYESLRDRFLEYYARELCVDTKLFDGIPELLGTLRER
                     GVAWGVVTNKPGRFTVPLMRQLDPNGTAACVVSGDTTPKSKPFPEPLLHAAREIDVPP
                     RECLYVGDDLRDVQAARAAGMGVVVAAWGYLGDAGDPAGWGADAVIAHPRETLNFI"
     gene            complement(703..1398)
                     /gene="ubiG"
                     /locus_tag="VI319_05135"
     CDS             complement(703..1398)
                     /gene="ubiG"
                     /locus_tag="VI319_05135"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY6863264.1"
                     /translation="MNADPAELAKFSALAHRWWDPTSEFRPLHEINPLRLDWIDSIAR
                     LAGKSALDVGCGGGILAEAMARRGARVKGIDLSEKALKVAELHGMESRAGVEYEQIAA
                     EALAAREPGRWDVVTCMELLEHVPDPESTVRACAQAVKPGGLVFFSTINRNPKSYLFA
                     VIGAEYLLRLLPRGTHDYAKFIRPSELARWCRGAGLETREIKGMTYNPLTKVYALGRD
                     TDVNYLVCATKAA"
     gene            complement(1468..1986)
                     /locus_tag="VI319_05140"
     CDS             complement(1468..1986)
                     /locus_tag="VI319_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6863265.1"
                     /translation="MNKAVRSAFAASLLFVAACASQQPAPPPAPAPAPEVTAPKPEVA
                     PAAVEAPAPKPLPEKVTLQADALFDFDKSAIRADAQGRLDDVVARAKGVSVEAVNVVG
                     HTDSIGSDEYNMKLSQRRAASVKDYLVKHGVPASRVHTQGKGESAPIADNKTREGRQK
                     NRRAEIEVLGTR"
     gene            complement(2086..2874)
                     /locus_tag="VI319_05145"
     CDS             complement(2086..2874)
                     /locus_tag="VI319_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6863266.1"
                     /translation="MSTTRGLRFHPTLVAASVAAAFATIAHDASAQAVVERDATTRPT
                     IVDRADASRYVTTNQGNVVVSGTGLCVHTGSWTPGTSVAGCDPLPRRVAVAPEPQPEP
                     PAPAAAAPAEPVAPPQQLAEPTPAPLPEKLSLQTDALFNFDKSDLRPDAKAELDNLLE
                     RIRASNVDEIDLVGHTDSIGTDQYNQKLSERRAEAVKAYLVSQGMAAEKIHTEGRGER
                     EPVADNKTKEGRQENRRVDVAVAGTTTQPQVVGQTPSESTTRTQ"
     gene            complement(2950..4275)
                     /locus_tag="VI319_05150"
     CDS             complement(2950..4275)
                     /locus_tag="VI319_05150"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEY6863267.1"
                     /translation="MENADLLISARWVIPVEPAGAVLADHAVAVRGGRIAAVLPAAEA
                     ERRYAAAARVRLDEHALIPGLVNLHTHAALTLMRGLADDLPLMEWLKDHVWPAEAKHV
                     SHDFVYDGTLLACAEMLRGGVTCFSEMYFFPDAAAKAALEARMRAVIGIIAIELPTAY
                     ASDADDYIAKGLAARDAFRDEKRLSFSMAPHAPYTVADRTFARMLTIAEELDLPIHVH
                     LHETEDEIGQSLAQHAKRPLERLRALGLVGPRLIAVHGIHLTREEIALLARFGASVAH
                     CPSSNLKLASGFAPVAAMIDAGVNVGIGTDGAASNNRLDMFEEMRTAALLAKAVARKA
                     DALPAHQALAAATLAGARALGLDRSIGSLVPGKCADLCAVSLSDVELSPVYDPISHLV
                     YAAGREHVTHVWCEGELLVEEGRLIRWSRPDLLRRAHSWRDRLAQDQKG"
     gene            complement(4329..4898)
                     /locus_tag="VI319_05155"
     CDS             complement(4329..4898)
                     /locus_tag="VI319_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6863268.1"
                     /translation="MSTEQTLKSFIVAGLIVLAGCASQAPAPAPAPAPAPRSEPAPTV
                     SAPTQQVAPAAVATPAKRCDATVTLTNNETFAFRKADLTAAAKARLDRDVVAKLAGCS
                     RVEAIVIEGHADRLGTQEFNQKLSEQRAEAVRAYLALHGAANDPIETIGMGKTVPAKF
                     CPDVKDRAKLIDCLAPNRRAIVTIKGTAR"
     gene            5036..7648
                     /gene="gyrA"
                     /locus_tag="VI319_05160"
     CDS             5036..7648
                     /gene="gyrA"
                     /locus_tag="VI319_05160"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY6863269.1"
                     /translation="METFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMFETSNVWNRPYVKCARVVGEVLGKYHPHGDAATYEALVRMAQDFSMRYVL
                     IDGQGNFGSIDGDSAAAYRYTECRLDRIASEMLADIEKETVDFVPNYDGKEREPVTLP
                     TRVPNLLINGSSGIAVGMATNIPPHNLGEVVDACQALLKSPEIAIDDLIELMPAPDFP
                     TAGIIYGVAGVREGYRTGRGRIVIRARTHFEELDRGNRQAIVIDEIPYQLNKTTLLQR
                     IGELVNEKKLEGISHIQDESDKSGMRVVLELKRDALPEVVLNNLFKQTQLQDSFGMNM
                     VALVDGQPRLLNLKEMLEAFLAHRREVITRRTLYDLREARKKAHIQEGLAVALSNVDE
                     IIALIKASKTPPEAKAALMAKAWRSSVVAEMLQRTTLEVSRPDDLPEGLGMGPDGYRL
                     SDAQAQAILEMRLQRLTGLEQEKIVEEYKSLIAQITDFLDILAKPARITAIIASELAA
                     IKEQYGDKRKSEIVVQAEDLSMEDLIAAEDVVVTFSHSGYIKAQPLTDYRAQRRGGRG
                     KQAATTKEDDFIERLFIANTHDTMLCFSSRGRVYWLKVYNVPQGTRTSRGKPIVNLFP
                     LGEAEKITAVLPIKEYDENHFVFMATAQGTVKKTRLPEFSNPREKGIIAVELDDGDHL
                     IGAAITDGKHDVMLFSDAGKAVRFEEDDVRAMGRQARGVRGMKLADDGSQRVISMLVA
                     ADEAQTVLTATENGFGKRTAIAEYTRHGRGTQGMIAIQTSERNGKLVSAVLVKDEDEI
                     MLISTGGVLIRTRVDQIREMGRSTQGVVLINLDEGTKLAGIECIAEREESEGNGNGNG
                     HGGNGSGDGQPAGE"
     gene            7626..>8022
                     /locus_tag="VI319_05165"
     CDS             7626..>8022
                     /locus_tag="VI319_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6863270.1"
                     /translation="MGNRPVNRVYNFSAGPAALPEEVLSQAAAEMLDWHGSGMSVMEM
                     SHRGKEFIAIAADAEKDLRDLLAVPNNYKVLFLQGGATLQFAAVPMNLLGGKKKADYV
                     NTGEWSKKAIKEANKYCEVNVAAGSDDKNF"
BASE COUNT         1257 a         2717 c         2819 g         1229 t
ORIGIN      
        1 gggttgtcga tgcgcgatct tgcgacccag taagcgggca ttgccggctc ctcaaatgaa
       61 attcaaagtc tcgcgcgggt gcgcgatcac cgcgtcggcg ccccagcccg ccgggtcgcc
      121 ggcgtcgccg agatagcccc acgccgcgac gaccacgccc atgccggcgg cgcgcgccgc
      181 ctgcacgtcg cgcaggtcgt cgcccacgta gaggcactcg cgcggcggca cgtcgatctc
      241 gcgcgccgcg tgcagcagcg gctcggggaa cggcttcgat ttcggcgtcg tgtcgccgct
      301 gacgacgcat gccgcggtgc cgttcggatc gagctgccgc atgagcggca cggtgaagcg
      361 cccgggcttg ttcgtgacca cgccccaggc gactccgcgc tcgcgcagcg tcccgagcag
      421 ctcggggatg ccgtcgaaga gcttcgtatc gacgcacagc tccctggcgt agtactcgag
      481 gaagcggtcg cgcagcgact cgtacgtcgc gtgctcgggg gcgacgccca tgccgacacc
      541 gagcagcccg cgcgcgcctt tcgaggtgtg cggccgcgtc atggccgggg gcacggggcc
      601 gaggccgcgc tcggcgcgca cgctgttgag cgcgcgcgcg aggtcgctcg ccgtgtcggc
      661 gagcgtgccg tcgaggtcga acagcaccgc gcgtaccgcc attcaggcgg ccttcgtcgc
      721 gcagacgaga tagttgacgt cggtgtcgcg cccgagcgcg tagaccttgg tcagcgggtt
      781 gtaggtcatg cccttgatct cgcgcgtctc gaggccggcg ccgcggcacc agcgcgcgag
      841 ctcggacggg cggatgaact tcgcgtagtc gtgcgtcccg cgcggcagca gccgcagcag
      901 gtattcggcc ccgatcactg cgaacaggta cgacttcgga ttgcggttga tggtcgagaa
      961 gaacacgagc ccgcccggct tcaccgcctg cgcgcaggcg cgcaccgtgc tctcggggtc
     1021 ggggacgtgc tcgagcagct ccatgcaggt gacgacgtcc cagcggccgg gctcgcgcgc
     1081 cgcgagcgcc tcggccgcga tctgctcgta ctcgacgccg gcgcggctct ccatcccgtg
     1141 cagctcggcg accttcagcg ccttctccga caggtcgatg cccttcacgc gcgcgccgcg
     1201 ccgcgccatc gcctccgcga ggatcccgcc gccgcagccg acatcgagcg ccgactttcc
     1261 ggcgagccgg gcgatcgagt cgatccagtc gaggcgcagc gggttgatct cgtgcagcgg
     1321 ccggaactcg ctggtcgggt cccaccagcg gtgcgcaagc gcgctgaact tggcgagctc
     1381 tgcgggatcg gcgttcatgg cgccatcata cgacgcaacg aatgtcgcgt cgcccccgcg
     1441 gaatccccgc tttcgcgggg atgactgcta acgcgtgccg agcacctcga tctcggcgcg
     1501 ccggttcttc tggcggcctt cgcgcgtctt gttgtcggcg atcggagcgc tttcgccctt
     1561 gccctgcgta tggacgcggc tcgcgggcac gccgtgcttc accaggtagt ccttcaccga
     1621 cgccgcgcgc cgctgcgaga gcttcatgtt gtactcgtcg ctgccgatcg agtcggtgtg
     1681 ccccacgacg ttgaccgcct cgaccgacac gcccttcgcc cgggcgacga cgtcgtcgag
     1741 gcgcccctgc gcgtcggcgc ggatcgccga cttgtcgaag tcgaacagcg cgtcggcctg
     1801 cagcgtcacc ttctcgggca agggtttcgg cgcgggcgcc tcgaccgcgg cgggcgcgac
     1861 ctcgggcttg ggcgccgtga cctcgggcgc cggtgcgggc gccggcggcg gggccggctg
     1921 ctggctcgcg caggcggcca cgaagagcag ggaagcggcg aatgcggatc ggacggcttt
     1981 gttcatgttt tccccccggg ctttgttgtg gcgccgatga tacgccggga cgccgcaatg
     2041 aaaaagcccc gccggagcgg ggcttcgtgg accagcgggc ggcgtctact gtgtcctcgt
     2101 cgtcgactcg gacggcgtct ggccgacgac ctgcggctgg gtcgtcgtcc cggcgaccgc
     2161 gacgtcgacg cggcggttct cctgccggcc ttccttcgtc ttgttgtcgg cgaccggctc
     2221 gcgctcgccg cggccctcgg tgtggatctt ctccgccgcc atgccctggc tgacgagata
     2281 ggccttcacc gcttcggcgc ggcgctcgga cagcttctgg ttgtactggt ccgtgccgat
     2341 gctgtcggtg tggccgacga ggtcgatctc gtcgacgttc gacgcgcgga tgcgctcgag
     2401 cagattgtcg agctcggcct tggcatcggg ccgcagatcc gacttgtcga agttgaacag
     2461 cgcgtcggtc tggagcgaga gcttctccgg cagcggcgcg ggcgtcggct cggcgagctg
     2521 ttgcggcggc gcgaccggct cggcgggcgc ggccgcggcg ggcgcgggcg gctcgggctg
     2581 gggctccggc gcgacggcaa cgcggcgcgg cagcggatcg cagcccgcga cgctcgtgcc
     2641 cggcgtccac gatcccgtgt gcacgcatag ccccgtgccg ctgacgacca cgttgccctg
     2701 gttcgtcgtc acgtagcgcg acgcgtcggc gcggtcgacg atcgtcggtc gggtggtcgc
     2761 gtcgcgctcg acgaccgcct gcgcggaagc gtcgtgcgcg atcgttgcaa aggcggccgc
     2821 caccgaagcg gcgaccagcg tcggatgaaa acgaagtcct cgagtcgtgc tcatagttcc
     2881 tctggaaagg ttgcgcgatg cacttgcagg cgcgcctccc cagaatgccc gcggtcgcga
     2941 gctctcggct tagccttttt ggtcttgcgc caatcggtcg cgccacgaat gcgcacgacg
     3001 cagcaaatcc ggcctgctcc accgaatcag acgaccctct tccacgagaa gttccccctc
     3061 acaccagaca tgcgtgacgt gctctctgcc cgcggcatag accagatggg agatgggatc
     3121 gtagaccggc gaaagctcga cgtccgagag cgacaccgcg cacaggtcgg cgcacttccc
     3181 tggaaccaat gatccgatcg agcgatcgag cccgagcgcg cgcgcgcccg cgagcgtcgc
     3241 ggccgcgagc gcctggtgcg cgggcagcgc gtcggccttg cgcgcgaccg ccttcgcgag
     3301 cagcgccgcg gtgcgcatct cctcgaacat gtcgaggcgg ttgttgctcg ccgcgccgtc
     3361 ggtgccgatg ccgacgttga cgccggcgtc gatcatcgcc gcgaccggcg cgaagccgct
     3421 cgcgagcttc aggttcgacg acgggcagtg tgcgaccgag gccccgaagc gcgcgagcag
     3481 cgcgatctcc tcgcgcgtca agtggatgcc gtgcaccgcg atcagccgcg gtcccacgag
     3541 cccgagcgcg cgcaggcgct cgagcgggcg cttcgcgtgc tgcgcgaggc tctgcccgat
     3601 ctcgtcctcg gtctcgtgca ggtgcacgtg gatcggcagg tcgagctcct cggcgatcgt
     3661 cagcatgcgc gcgaaggtgc ggtcggcgac ggtgtagggc gcgtgcggcg ccatgctgaa
     3721 cgagagccgc ttctcgtcgc ggaaggcgtc gcgcgccgcc aggcccttcg cgatgtagtc
     3781 gtcggcgtcg ctcgcgtacg cggtcggcag ctcgatcgcg atgatgccga tcaccgcgcg
     3841 catccgcgcc tcgagcgccg ccttcgccgc cgcgtcgggg aagaagtaca tctcgctgaa
     3901 gcaggtcacc ccgccgcgca gcatctcggc gcaggcgagc agcgtgccgt cgtatacgaa
     3961 gtcgtgcgac acgtgcttcg cctcggccgg ccagacgtgg tccttgagcc actccatcag
     4021 cgggaggtcg tcggcgagcc cgcgcatcag cgtcagtgcc gcgtgcgtgt gcagattgac
     4081 gagtcccggg atgagcgcgt gctcgtcgag ccgcacgcgc gcggcggcgg catagcgccg
     4141 ctcggcctcc gccgccggca ggaccgcggc gatgcggccg ccgcgcaccg cgaccgcgtg
     4201 gtcggcgagc acggcacccg ccggctcgac cgggatcacc cagcgcgcgc tgatgaggag
     4261 gtcggcgttt tccatcgcga aaagaaaagg ccccgcggac ggggcctttc gggttacggc
     4321 agtttaaatt acctggccgt gcccttgatg gtcacgatcg cgcggcggtt gggcgcgagg
     4381 cagtcgatca gcttcgcgcg gtccttgacg tcgggacaga acttcgccgg gacggtcttg
     4441 cccatgccga tcgtctcgat cgggtcattg gccgcgccgt gaagggcaag ataggcgcgc
     4501 acggcctcgg cgcgctgctc cgagagcttc tggttgaatt cctgggtgcc gaggcggtcg
     4561 gcgtggccct cgatcacgat cgcctcgacg cggctgcagc ccgcgagctt ggcgacgacg
     4621 tcgcggtcga gccgcgcctt cgccgcggcc gtgaggtcgg ccttgcggaa ggcgaaggtc
     4681 tcgttgttgg tcagcgtcac ggtcgcgtcg cagcgcttcg ccggggtcgc gaccgcggcg
     4741 ggcgcgacct gctgcgtcgg cgctgagacc gtcggcgcgg gctcggatcg gggcgcgggc
     4801 gccggcgcgg gtgccggagc gggcgcctgg ctggcgcagc cggcgagaac gatgaggccg
     4861 gcgacgatga acgacttcag ggtctgttct gtggacatct ttttcctccc tggatcggct
     4921 tgtcggtgcg ccggaatcat acaccaatcg cgcttgggtc cggccctcgc aagctgctaa
     4981 aatttatgtt ttttcgagcg cccctttccg gtccgcattt ttcgttcaga cctcgatgga
     5041 aaccttcgca aaagaaacgc tccccgtcag cctcgaagag gagatgcggc gctcgtatct
     5101 cgactacgcg atgagcgtca tcgtcgggcg cgcgctgccc gacgtgcgcg acggcctgaa
     5161 gccggtgcac cggcgcgtcc tctacgcgat gttcgagacg agcaacgtct ggaaccgccc
     5221 ctacgtgaag tgcgcgcgcg tggtcggcga ggtgctcggc aagtaccacc cgcacggcga
     5281 cgccgcgacc tacgaggcgc tggtgcgcat ggcgcaggac ttctcgatgc gctacgtgct
     5341 gatcgacggc cagggcaact tcggctcgat cgacggcgac tcggcggcgg cgtaccggta
     5401 tacggagtgc cggctggacc gcatcgcctc ggagatgctc gccgacatcg agaaggagac
     5461 ggtcgacttc gtcccgaact acgacggcaa ggagcgcgag ccggtcacgc tgccgacccg
     5521 cgtgccgaac ctgctgatca acggctcctc gggcatcgcc gtgggcatgg cgaccaacat
     5581 cccgccgcac aatctcggcg aggtggtcga cgcgtgccag gcgctgctga agagcccgga
     5641 gatcgccatc gacgatctca tcgagctgat gccggcgccc gacttcccga ccgcggggat
     5701 catctacggc gtcgccggcg tgcgcgaggg ctaccgcacc ggccgcggcc gaatcgtcat
     5761 ccgggcgcgc acgcacttcg aggagctcga ccgcggcaac cgccaggcga tcgtcatcga
     5821 cgagatcccg taccagctca acaagacgac cctgctgcag cgcatcggcg agctggtcaa
     5881 cgagaagaag ctcgagggca tcagccatat ccaggacgag tccgacaagt ccggcatgcg
     5941 cgtggtgctc gagctcaagc gcgacgcgct gcccgaggtg gtgctgaaca acctgttcaa
     6001 gcagacgcag ctgcaggaca gcttcggcat gaacatggtg gcgctggtgg acggccagcc
     6061 gcggctcctg aacctcaagg agatgctcga ggcgttcctc gcccaccgcc gcgaggtcat
     6121 cacccggcgc accctctacg acctgcgcga ggcgcgcaag aaggcgcaca tccaggaagg
     6181 cctcgcggtc gcgctctcga acgtcgacga gatcatcgcc ctcatcaagg cgtcgaagac
     6241 gccccccgag gcgaaggccg cgctgatggc gaaggcgtgg cgctcgagcg tcgtcgccga
     6301 gatgctccag cgcaccacgc tcgaggtctc gcgccccgac gacctgcccg aagggctcgg
     6361 catggggccc gacggctacc gcttgtcgga cgcgcaggcg caggccatcc tcgagatgcg
     6421 gctgcagcgc ctgaccggcc tggagcagga gaagatcgtc gaggagtaca agagcctgat
     6481 cgcgcagatc accgacttcc tcgacatcct ggcgaagccg gcgcgcatca ccgcgatcat
     6541 cgcctccgag ctcgccgcga tcaaagagca gtacggcgac aagaggaaga gcgagatcgt
     6601 ggtccaggcc gaggacctgt cgatggagga cctgatcgcg gccgaggacg tggtcgtgac
     6661 cttctcgcac tcgggctaca tcaaggcgca gccgctcacc gactaccgtg cgcagcggcg
     6721 cggcgggcgc ggcaagcagg cggcgacgac gaaggaagac gacttcatcg agcggctgtt
     6781 catcgccaac acgcacgaca cgatgctgtg cttctcgtcg cgcgggcgcg tctactggct
     6841 caaggtctac aacgtgccgc agggcacgcg caccagccgc ggcaagccga tcgtcaacct
     6901 gttcccgctc ggggaggccg agaagatcac cgcggtgctg ccgatcaagg agtacgacga
     6961 gaaccacttc gtgttcatgg cgacggcgca gggcacggtg aagaagacgc gcctgcccga
     7021 gttctcgaac ccgcgcgaga agggcatcat cgcggtcgag ctcgacgacg gcgaccatct
     7081 catcggcgcc gcgatcaccg acggcaagca cgacgtgatg cttttctccg acgccggcaa
     7141 ggcggtgcgc ttcgaggagg acgacgtgcg cgcgatgggc cgccaggcgc gcggcgtgcg
     7201 cggcatgaag ctcgccgacg acggctcgca gcgggtcatc tcgatgctgg tggcggccga
     7261 cgaggcccag accgtgctga ccgcgaccga gaacggcttc ggcaagcgca ccgcgatcgc
     7321 ggaatacacg cgtcacggcc gcggcacgca ggggatgatc gcgatccaga cctcggagcg
     7381 gaacggaaag ctggtgagcg ctgtcctcgt gaaggacgag gacgagatca tgctcatctc
     7441 cacgggcggt gttctcatcc gcacccgggt cgaccagatc cgagagatgg gccgatcgac
     7501 gcagggcgtg gtgctgatca acctggacga gggaaccaag ctcgcgggca tcgaatgcat
     7561 cgccgagcgc gaagagagcg aaggcaatgg caacggcaac ggacacggag gcaacggaag
     7621 cggcgatggg caaccggccg gtgaataggg tctacaactt cagcgcgggt cccgccgcgc
     7681 tccccgaaga ggtcctgtcg caggccgccg ccgagatgct cgactggcac gggtcgggca
     7741 tgtcggtgat ggagatgagc caccgcggca aggagttcat cgcgatcgcc gcggacgcgg
     7801 agaaggacct gcgcgatctg ctcgcggtgc cgaacaatta caaggtgctg ttcctccagg
     7861 ggggcgcgac gctgcaattc gccgccgtcc cgatgaacct gctcggcgga aagaaaaagg
     7921 ccgactacgt caatacgggc gagtggtcga aaaaggcgat caaggaggcg aacaaatatt
     7981 gcgaagtgaa cgtcgccgcc ggctccgacg acaagaattt ca
//
LOCUS       DATDMW010000117        14683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1402545, whole genome shotgun sequence.
ACCESSION   DATDMW010000117 DATDMW010000000
VERSION     DATDMW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14683
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402545"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VI319_05170"
     CDS             <1..286
                     /locus_tag="VI319_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6863271.1"
                     /translation="PRDAIHRAALLRFRPIMMTTMAALLGAVPLAIGFGEGAEMRRPL
                     GIAIVGGLIFSQALTLYTTPVVYLYVDRFRHWARRRRGLPPIDRLAHESR"
     gene            273..1685
                     /locus_tag="VI319_05175"
     CDS             273..1685
                     /locus_tag="VI319_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY6863272.1"
                     /translation="MNRASALSLAALALLSACSVGPDYVRPDAPVPQAFKETQGWKPA
                     EPRDAEVRGSWWEAFGDPLLNQLEEQVSVSNQNVAAAEARFRQAMALIGAARAQYFPA
                     ITGDVSATRSRSSSGSNSAVISRGVITSRSAAINADWELDLWGRVRRTVESNVAGAQA
                     SAGDLESARLSAQVQLAQSYFQLRQRDTERRLLDDTVQALGVSLELTRNRYAAGVAAK
                     VDVVQAQTLLKTTQAQAIDLGVQRAQLEHAIALLAGKAPADRSIPPGALGALPPPAPV
                     GLPSALIERRPDIAAAERRMAQANAQIGVAKAAYFPQLTLSGSIGYQSTSLANWFTAP
                     SRFWSVGPAVAATLFDAGLRRSQTEQAIANYDATVAAYRQTVLTAMQDVEDNVAALRI
                     LEEEAVVQKEALEAARQNLELTLNQYKGGVVSYLNVVTAQTTAIQNERTAVDLQSRRL
                     QASVLLVRALGGGWNASAAR"
     gene            complement(1687..2667)
                     /locus_tag="VI319_05180"
     CDS             complement(1687..2667)
                     /locus_tag="VI319_05180"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY6863273.1"
                     /translation="MEIRDGFVGTIGNTPLIRLRGPSKLTGCDILGKAEFLNPGGSVK
                     DRAALAIILDAERRGLLAPGGTVVEGTAGNTGIGLALVGNARGYRAVIVIPDTQSREK
                     KDMLRLCGAELREVPAVPYKDPNNYVHVSERLAKEMGAFWANQWDNTANRDAHYRGTG
                     PEIWAQTGGRVDGFTCAIGTGGTLSGVALYLKEKKPGIRIALADPMGAAMYEWFKNGV
                     LKSEGSSITEGIGQGRVTKNVEGTPVDEAYRIPDSEALPILFDLLRYEGLCLGGSSGI
                     NVAGAVRLAKDLGPGHTVVTILADSGTRYQSKLFDPQFLREKGLPAPGWL"
     gene            2716..3414
                     /locus_tag="VI319_05185"
     CDS             2716..3414
                     /locus_tag="VI319_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY6863274.1"
                     /translation="MLEIRKANERGYADHGWLRSFHTFSFADYYDPRHMGFGPLRVIN
                     EDRIKPGTGFGTHGHRDMEIISYVLEGALAHKDSMGTGSTIVPGDVQRMSAGTGVMHS
                     EFNHADGAVTHFLQIWIEPNVRGIAPSYEQKHFDAQSKRGKLRLVASPDGRDGSVRIH
                     QNAFLYAALLDGAERVSHALAEGRRGYVHVARGEVTVNGQPLEAGDALKATSTKEIVL
                     ENGRDAEVLLFDLA"
     gene            3426..3827
                     /locus_tag="VI319_05190"
     CDS             3426..3827
                     /locus_tag="VI319_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6863275.1"
                     /translation="MTGQNLAALVGRVLLVLIFLGSGLDKVMHPDATLAYMNSAHLPA
                     AQLLLWASVVVEIGAGLAMALGWKARWAAALLFLWMIPGTAVVHNPWAGDPSQAQMQM
                     VHLMKNISIMGGLLLALAFGPGGWSVDRRKA"
     gene            3832..4218
                     /locus_tag="VI319_05195"
     CDS             3832..4218
                     /locus_tag="VI319_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6863276.1"
                     /translation="MTDRLARNKANVQAFYDLAFNQCKAADAMTKYAGDVYIQHNPAV
                     ANGKQAFVDYFTRMAAEYPGKRVHFVRVIAEGDYVVLHCRQEWPGDSDWAGIDIFRCN
                     DQGKVVEHWDVLQRVPVKSANGNTMF"
     gene            4230..5012
                     /gene="ygiD"
                     /locus_tag="VI319_05200"
     CDS             4230..5012
                     /gene="ygiD"
                     /locus_tag="VI319_05200"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEY6863277.1"
                     /translation="MSSTIPAVFFGHGSPMNALARNEYSEAWRRVGESMPKPRAVLAV
                     SAHWYTRGTLVTADEHPKTVHDFYGFPKALFDVQYPAPGDPALVERVRELLAPVPVRP
                     DRSWGFDHGTWSVLVHVYPKADVPVLQLSIDATQPPQFHYDLGRRLAPLRDEGVLLAG
                     FGNVVHNLQAMRRTEDATAFDWAARFESAIRKALIGRDHDTLVHWERLGDDARLSVPT
                     PEHYLPLLYVAGAQRDAEQVAIAVDGVDLGSISMLTAVVGSA"
     gene            5062..6867
                     /locus_tag="VI319_05205"
     CDS             5062..6867
                     /locus_tag="VI319_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY6863278.1"
                     /translation="MHLLEIAGIFLLTAVLLVPLFRRLGLGAVLGYLAAGMLIGPWGL
                     GLAGDVDATLEFAEFGVVLLLFLIGLELEPRRLWVLRHSVFGLGGAQVAVTGLVLGVA
                     ALALRYDWRAALVIGLGLAMSSTAMVLTSLSERGQLADRHGRVAFAVLLFQDLSVIPL
                     LALLPWLAASESAGVGGWIAAAKGLAVIVAVVAASRILVRPALKAVARWGGQDVFTAA
                     ALLMVVGASLAMTWIGLSMSLGAFLAGVLLADSEFRHELEADIEPFKGLLLGLFFMAV
                     GMSANLELLAERPLLVVGLALALVVVKFVLVFGIARVFGHERDPAWRLAVALAQGGEF
                     AFVLFALAGSLGVLASAASELLVLVVTVSMLLAPLAFAVQDRIVDPWLAQRGAPEFDV
                     IDGPGNPVIIAGYGRVGQVVSRVMRMVGLSFTALESSFQQVDFVRRYGGKVYYGDASR
                     LELLNAAKAGEARLFVLAIDDVDASVKTAGVVRRHFPDLPIIARARNRTHYFQLRNLG
                     IKNIFRETFPASVDMARLALLMLGMAPAATERTIALFRSHDEERLDAQYAVRQDEARL
                     IQSTREAADQLREVFETDIGPEAGFGQRASKERAA"
     gene            6864..7742
                     /locus_tag="VI319_05210"
     CDS             6864..7742
                     /locus_tag="VI319_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY6863279.1"
                     /translation="MRRRAWAAVLVLGLLLPGAAAAERFAHGMLWRVSRDGVAPSYVF
                     GTIHVADPRVLDLPEPVGRALGRSRRLYVETQLGAREEARFFEAAQFEDGRRLEPLIG
                     AEAYAGVAAELRRRNVPEEVIARIKPWAALVNLTVQPEDYDGATLDQRIAAIARAHGA
                     RVLGLEGIEEQIAVFEHIPLDTQIALLKHALAHRDELAAMIEPTIQAWMRRDLAGLHA
                     ASERVEERYPEMAPHYRILFRSVVDNRTVVMAHRLFMPLREGRAFVAVGANHLYGEHG
                     LLALIAEQGYRVTRVY"
     gene            complement(7761..8792)
                     /locus_tag="VI319_05215"
     CDS             complement(7761..8792)
                     /locus_tag="VI319_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6863280.1"
                     /translation="MKYPLKRLQDQVIVITGASSGIGLTTARYAAKRGAKLVLASRNE
                     KALKQLTGELAKCYGCQSIYVVADVGKEKDVRDIAAQALKRFDRFDTWINNAGVSIYG
                     RIEQIPVEDQRKLFETNFWGVVYGSIVAVEHLKQHGGALINLGSEVSDVAIPLQGIYS
                     ASKHAVKGFTDSLRLELEQEKAPVSVTLIKPAAVDTMYVAHAKNFMEVEPKLPGPIYA
                     PELVADAILIASQYPKRDIFVGGAAKFFSSAAHYAPRMMDKFLQSFMFKRQLTARPAR
                     AVGDDGLHQSGGTALQERQGQESHVYSSSVYTRAVTRPKSTAAVLLAAGLVAAVVWQL
                     LRPAGARSR"
     gene            8889..9539
                     /locus_tag="VI319_05220"
     CDS             8889..9539
                     /locus_tag="VI319_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6863281.1"
                     /translation="MDRKKDSRMSWLMSALYDRFMRQSEAACLGRWRAELLHDLSGSV
                     LEIGAGTGASLTHYPASVSRLVLCEPDPHMRRKLQARAAAVAPGRTEVADCSAQALGF
                     PSASFDAVVSSLVLCSVSDPDAALAEVARVLKPRGRLVFLEHVAADSNPPRLKWQKRI
                     EPVWKHLMGNCHLTRRTEAVIGAAGFRIERIQRESIRKAMPLVRPSIRGIAVKMTA"
     gene            9536..10162
                     /gene="purN"
                     /locus_tag="VI319_05225"
     CDS             9536..10162
                     /gene="purN"
                     /locus_tag="VI319_05225"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY6863282.1"
                     /translation="MKSIVVVISGRGSNMEALVRADLPARIAAVVSNRPEAAGLRFAA
                     ERGIPTAVVDHERYPSRDAFDAALAEALERHRPDLVALAGFMRVLGGDVVRRYEGRML
                     NIHPSLLPSFQGLHTHARALEQGVKVHGCTVHFVTPALDHGPIVIQAAVPVHPGDDET
                     ALAARVLRQEHVIYPRAVRWFLEGRLVIEEGRVRVTGSDERQLLWSDE"
     gene            10159..10848
                     /locus_tag="VI319_05230"
     CDS             10159..10848
                     /locus_tag="VI319_05230"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY6863283.1"
                     /translation="MRHRWLVLAALAPTALCAAPPHHVVVEYDMSRNDMVMAQLKETL
                     DHDGKSYRIVSEGKATGIFAVLAPGTVLRTSEGAVAAEGLLPREYREQRGSKSASARF
                     DRSAHTLVQQREGQAAETQPLPERAQDRLAYLWSFAFVPPKSGHVEAMVADGRGAPVR
                     YDYAVAGGETLKTPMGDLQTLHLVKQRTPDDSRQTEVWLDEKRNFVPVRVLVVEKDGT
                     RLDQVVTRIEE"
     gene            10857..12158
                     /locus_tag="VI319_05235"
     CDS             10857..12158
                     /locus_tag="VI319_05235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY6863284.1"
                     /translation="MAVPSAVETARAESSRRYSRALLDACCGALAAVLPLERAADASL
                     SAYFRDHPALGQRDRALVADTVYAVLRRRRLLERVAGATGARRLVLAAWAVLLGANLR
                     ELEPLARGDEPGWLREVKREAHAPQPFEVECELPDWLIERLRTRFDDAGLLEIARGLL
                     APAPLDLRVNVAKASREQVLERLRAEGMGAEPTPFSPDGMRVAGHPAINRHPLFADGA
                     VEVQDEGSQIVCRLLAPRRGEMVVDFCAGAGGKTLALGALMRSSGRLYAFDVSDARLA
                     KLKPRLARSGLSNVHPQRLAGENDARVKRLAGKIDRVLVDAPCTGLGTLRRNPDLKWR
                     QTPGDVVELAAKQRAILESAARLVKPGGRLVYATCSILDEEDRGVVEPVLERHPEFAL
                     RPAGEALAQERIAIEADRFLELWPHRHGTDGFFAAVLERSA"
     gene            12155..13426
                     /locus_tag="VI319_05240"
     CDS             12155..13426
                     /locus_tag="VI319_05240"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY6863285.1"
                     /translation="MRNLVYEAWLDLQHPEVVWQIGALALALAVSWLVNRLARFDRIE
                     ASGVWKFGVGGLKRILAPVVALLIVIVAREVLIRHVRVSVDLLHLAVPLLGSLALVRV
                     FFYALRHVFAPGGVLGRLERTLAAIVWVVFALYIVGALPTVVAGLDDAGFTLGRYRIS
                     AWLVLQAAFWTFVTLLGALWSGSAIEARLMRAEGLHYSLRVALSRVSRALLVLIALLV
                     VLPLVGIDITVLSVFGGALGVGLGFGLQKVAGNYVSGFVVLLERSIRIGDMITADNFH
                     GKVLDITSRYTLVRAQDGREAIIPNETLITSTVLSHTHSDRMLRVACPVSVAYGTDLD
                     RAMSTLRDVALAHPRVLRDPEPGALVLRFGDSGIELELGFWIGDPEQGTSNVRSEINL
                     AIWRGLRDAGIEIPYPRRVIQITGGNSEARS"
     gene            13509..14683
                     /locus_tag="VI319_05245"
                     /pseudo
     CDS             13509..14683
                     /locus_tag="VI319_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827753.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
BASE COUNT         2056 a         5151 c         5163 g         2313 t
ORIGIN      
        1 gccgcgcgac gcgatccacc gcgccgcgct gctgcgcttc cgcccgatca tgatgacgac
       61 catggcggcg ctgctcggcg cggtcccgct cgcgatcggc ttcggcgaag gcgccgagat
      121 gcgccgcccg ctcgggatcg ccatcgtcgg cgggctcatc ttcagccagg cgctcacgct
      181 ttatacgact ccggtcgtct atctctacgt cgatcgcttc cggcactggg cgcgccgccg
      241 ccgcggcctg ccgccgatcg atcgtctcgc ccatgaatcg cgctagcgca ctctcgctcg
      301 cggcgctcgc gctgctctcc gcctgctcgg tcggccccga ctacgtccgc cccgacgcgc
      361 cggtgccgca ggcgttcaag gagacgcagg gctggaagcc cgccgagccg cgcgacgccg
      421 aggtccgcgg cagctggtgg gaggccttcg gcgatccgct gctgaaccag ctcgaggagc
      481 aggtgagcgt ctcgaaccag aacgtcgccg cggccgaggc gcgcttccgc caggcgatgg
      541 cgctcatcgg cgcggcgcgc gcgcagtact ttccggcgat caccggcgac gtgtcggcga
      601 cgcgctcgcg ctcgtcgagc ggttcgaact cggcggtgat ctcgcgcggc gtgatcacga
      661 gccgcagcgc ggcgatcaac gccgactggg agctcgacct ctggggccgg gtgcgccgca
      721 cggtcgagtc gaacgtcgcg ggcgcgcagg cgagcgccgg cgacctcgag tcggcgcgcc
      781 tgtcggcgca ggtgcagctc gcgcagagct atttccagct gcgccagcgc gataccgagc
      841 ggcggctcct cgacgacacg gtccaggcgc tcggcgtctc gctcgagctc acgcggaacc
      901 gctacgccgc gggcgtcgcg gcgaaggtcg acgtggtgca ggcgcagacg ctgctcaaga
      961 ccacgcaggc gcaggcgatc gacctcggcg tgcagcgcgc gcagctcgag cacgcgatcg
     1021 cgctgctcgc gggcaaggcg cccgccgacc gctcgatccc gcctggggcg ctcggcgcgc
     1081 tgccgccgcc cgcgcccgtc gggctgccgt ccgcgctgat cgagcgccgg cccgacatcg
     1141 ccgcggccga gcggcgcatg gcgcaggcga acgcgcagat cggcgtggcg aaggccgcct
     1201 atttcccgca gctcacgctg tcgggctcga tcggctacca gtcgacgagc ctcgccaact
     1261 ggttcaccgc gccgtcgcgc ttctggtcgg tcggcccggc ggtcgccgcg acgctgttcg
     1321 acgccggcct gcgcagatcg cagaccgagc aggcgatcgc gaactacgac gccacggtcg
     1381 cggcgtaccg ccagaccgtg ctcaccgcga tgcaggacgt cgaggacaac gtcgcggcgc
     1441 tgcgcatcct cgaggaggag gccgtcgtgc agaaagaagc gctcgaggcc gcgcgccaga
     1501 atctcgagct cacgctcaac cagtacaagg gcggcgtcgt gagctacctc aacgtcgtca
     1561 cggcgcagac caccgcgatc cagaacgagc gcaccgccgt cgacctccag tcgcgcaggc
     1621 tgcaggcgag cgtgctgctg gtgcgggcgc tggggggcgg ttggaacgcg tccgccgcgc
     1681 gctgagctag agccagcccg gagccggaag gcctttctcc ctcaggaact ggggatcgaa
     1741 cagcttggac tggtagcggg tgcccgagtc cgccaggatg gtgacgacgg tatgtccggg
     1801 tcccaggtcc ttcgcgaggc gcaccgctcc ggcgacgttg atcccgctcg atccgcccag
     1861 gcagagtccc tcgtacctca gcagatcgaa caggatcggc agcgcctccg aatccgggat
     1921 gcgatacgcc tcgtcgacgg gcgtgccttc gacgttcttc gtcacgcgcc cctggccgat
     1981 gccttcggtg atcgacgagc cctccgattt cagcacgccg ttcttgaacc actcgtacat
     2041 cgccgcaccc atcggatcgg cgagcgcgat ccggataccg ggcttcttct ccttgaggta
     2101 gagcgcgacc cccgagagcg tcccgccggt gccgatcgcg cacgtgaacc cgtcgaccct
     2161 cccgcccgtc tgcgcccaga tctccggccc cgtgccgcgg tagtgcgcgt cgcggttggc
     2221 ggtgttgtcc cactggttcg cccagaaggc gcccatctcc ttcgccaggc gctcggagac
     2281 gtgcacgtag ttgttcgggt ccttgtaagg caccgccggc acttcgcgca gctcggcgcc
     2341 gcacaggcgc agcatgtcct tcttttcccg gctctgggtg tcggggatga cgatgacggc
     2401 gcggtagccg cgcgcgttgc cgacgagcgc gaggccgatg ccggtgttgc ccgcggtgcc
     2461 ctcgacgacg gtgccgcccg gcgcgagcag gccgcgccgc tctgcgtcta gaatgatggc
     2521 gagcgcggcg cggtccttga ccgagccgcc cgggttcagg aactcggcct ttccgaggat
     2581 gtcgcacccg gtgagcttcg aaggtccgcg cagccggatc agcggcgtgt tgccgatcgt
     2641 gccgacgaag ccatcgcgga tttccatggg gcaggccaga ctttcgtctc catcataatc
     2701 gaagaaaaag ccgacatgct cgagatccgc aaagcgaacg aacgtggcta cgccgaccac
     2761 ggctggctgc gctccttcca caccttctcc tttgccgact actacgatcc gcgccacatg
     2821 ggcttcgggc cgctgcgcgt gatcaacgag gaccgcatca agccggggac gggcttcggc
     2881 acgcacggcc accgcgacat ggagatcatc agctacgtgc tcgagggcgc gctcgcgcac
     2941 aaggacagca tgggcacggg ctcgaccatc gtcccgggcg acgtgcagcg catgagcgcg
     3001 ggcaccggcg tgatgcacag cgagttcaac cacgccgacg gcgcggtcac gcacttcctg
     3061 cagatctgga tcgagccgaa cgtccgcggc atcgcgccga gctacgagca gaagcacttc
     3121 gacgcgcaat cgaagcgcgg caagctgcgc ctcgtcgcct cgcccgacgg gcgcgacggc
     3181 tcggtgcgca tccaccagaa cgccttcctc tacgcggcgc tgctcgacgg cgccgagcgc
     3241 gtgtcgcacg cgctcgccga agggcggcgc ggctatgtgc acgtcgcgcg cggggaggtt
     3301 acggtgaacg gccagccgct cgaagccggc gacgcgctca aggccacgag caccaaggag
     3361 atcgtcctgg agaacggccg ggacgccgaa gtcctgctct tcgacctcgc atagggagac
     3421 ccaatatgac cggacaaaac ctcgcggcgc tcgtcgggcg cgtgctgctc gtgctcatct
     3481 tcctcgggtc gggcctcgac aaggtgatgc accccgacgc gacgctcgcg tacatgaaca
     3541 gcgcgcacct gcccgccgcg cagctgctgc tctgggcctc ggtggtggtc gagatcggcg
     3601 cgggcctcgc catggcgctc ggctggaagg cccgttgggc cgcggccttg ctcttcctct
     3661 ggatgatccc gggtaccgcg gtcgttcaca atccgtgggc gggcgacccg tcgcaggcgc
     3721 agatgcagat ggtccacctg atgaagaaca tctcgatcat gggcggcctg ctgctcgcgc
     3781 tcgcgttcgg gccgggcgga tggagcgtcg atcggagaaa ggcgtgacgc cgtgaccgac
     3841 cggctcgcgc gcaacaaggc gaacgtccag gcgttctacg acctcgcgtt caaccagtgc
     3901 aaggccgccg acgcgatgac gaaatacgcg ggcgatgtct acatccagca caaccccgcg
     3961 gtcgcgaacg gcaagcaggc gttcgtcgac tacttcacgc gcatggcggc ggagtacccg
     4021 ggcaagcgcg tgcatttcgt gcgcgtgatc gccgagggcg actacgtcgt cctgcattgc
     4081 cgccaggagt ggccgggcga ctcggactgg gcgggcatcg acatcttccg ctgcaacgac
     4141 cagggcaaag tcgtcgagca ctgggacgtg ctgcagcgcg tccccgtgaa gtcggccaac
     4201 ggcaacacga tgttctgacg gagaacgcga tgtcgtcgac gattccggcg gtgttcttcg
     4261 gccatggcag cccgatgaac gcgctcgcgc gcaacgaata ctcggaggcg tggaggcgcg
     4321 tcggcgagtc gatgcccaag ccgcgcgccg tgctcgcggt ctccgcgcac tggtacacgc
     4381 gcgggaccct cgtgaccgcc gacgagcatc ccaagaccgt gcacgacttt tatggttttc
     4441 cgaaggcgct tttcgacgtc cagtatccgg cgccgggcga cccggccctg gtcgagcgcg
     4501 tgcgcgagct gctcgcgccg gtcccggtgc gcccggaccg ctcgtggggc ttcgaccacg
     4561 gcacctggtc ggtgctggtg cacgtctatc cgaaggccga cgtccccgtc ctgcagctca
     4621 gcatcgacgc gacgcagccg ccgcagttcc actacgatct cggccgcagg ctcgcgcccc
     4681 tgcgcgacga gggcgtgctg ctcgccggct tcggcaacgt cgtgcacaac ctgcaggcga
     4741 tgcggcgcac cgaggacgca acggccttcg attgggccgc gcgcttcgag tccgcgatcc
     4801 gcaaggctct gatcgggcgc gatcacgaca cgctcgtgca ctgggagcgc ctcggcgatg
     4861 acgcgcgtct ttcggtgccc acgcccgagc actacctgcc gctcctgtac gtggccggcg
     4921 cgcagcgtga cgccgagcag gtcgcgatcg ccgtcgacgg cgtcgatctg ggctccatca
     4981 gcatgctgac cgccgtcgtc ggctcggcgt aacgcagacg cggcgcggat gcggtaacgt
     5041 tcccgggtca cgggggacgc catgcatctg ctcgaaatcg ccgggatctt cctgctcacc
     5101 gcggtcctgc tcgtgccgct gttccggcgg ctcggcctgg gcgcggtgct cggctacctc
     5161 gcggccggga tgctgatcgg cccgtggggt ctcggcctcg cgggcgacgt cgacgcgacc
     5221 ctggagttcg ccgagttcgg cgtcgtcctg ctgctgttcc tgatcgggct cgagctcgag
     5281 ccgcggcgtc tctgggtgct gcgccactcg gtgttcgggc tcggcggcgc gcaggtcgcg
     5341 gtgaccggcc tcgtcctcgg cgtcgccgcg ctcgcgctgc gttacgactg gcgcgcggcg
     5401 ctcgtgatcg gcctggggct cgccatgtcg tcgaccgcga tggtgctgac ctcgctttcg
     5461 gagcgcggcc agctcgccga ccgccacggg cgcgtcgcgt tcgcggtgct gctgttccag
     5521 gacctgtccg tgatcccgct gctcgcgctg ctgccgtggc tcgccgcgtc cgaatccgcc
     5581 ggcgtgggcg gctggatcgc ggcggcgaag ggcctggcag tgatcgtcgc ggtggtcgcc
     5641 gcgagccgaa tcctcgtgcg gcccgcgctg aaggcggtcg cccgctgggg cgggcaggac
     5701 gtgttcaccg cggcggcgct gctgatggtc gtcggggcgt cgctcgcgat gacgtggatc
     5761 gggctgtcga tgtcgctcgg cgccttcctc gccggcgttc tgctcgccga ctccgagttc
     5821 cgccacgagc tcgaggccga catcgagccg ttcaaggggc tgctgctcgg gctgttcttc
     5881 atggcggtcg gcatgagcgc caacctcgag ctcctcgcgg agcggccgct cctcgtcgtc
     5941 ggtctcgcgc tcgcgctcgt ggtcgtgaag ttcgtcctgg tattcggcat cgcccgcgtc
     6001 ttcggccacg agcgcgatcc cgcctggcgg ctcgccgtgg cgctcgcgca gggcggcgag
     6061 ttcgcgttcg tgctgttcgc gctcgccggg tcgctcggcg tgctcgcgag cgcggcttcg
     6121 gagctgctcg tgctcgtcgt cacggtctcc atgctcctcg cgccgctcgc gttcgcggtc
     6181 caggaccgca tcgtcgatcc gtggctcgcg cagcgcggcg cgcccgagtt cgacgtcatc
     6241 gacgggccgg gcaacccggt gatcatcgcg ggctacggcc gcgtcggcca ggtggtctcg
     6301 cgcgtgatgc gcatggtggg cctctcgttc accgcgctcg agtcgagctt ccagcaggtg
     6361 gacttcgtgc ggcgctacgg cggcaaggtc tactatggcg acgcctccag gctcgagctc
     6421 ctgaacgcgg cgaaggcggg cgaggccagg ctgttcgtgc tcgcgatcga cgacgtcgac
     6481 gcgtcggtga agaccgcggg cgtcgtgcgc cggcattttc cggacctgcc gatcatcgcg
     6541 cgggcgcgca accggaccca ctacttccag ctgcgcaacc tcggcatcaa gaacatcttc
     6601 cgcgagacct tcccggcgag cgtcgacatg gcgcggctcg cgctgctcat gctcggcatg
     6661 gcgcccgctg cgaccgagcg caccatcgcg ctgttccgga gtcacgacga ggagcgcctc
     6721 gacgcgcagt acgccgtgcg ccaggacgag gcgcgcctga tccagagcac gcgcgaggcg
     6781 gccgatcagc tgcgcgaggt gttcgagacc gacatcggtc cggaagcggg attcggccag
     6841 cgcgcgtcga aggagcgggc ggcgtgaggc gaagagcgtg ggccgccgtg ctggtgctcg
     6901 ggctgctgct cccgggcgcg gcggcggcgg agcgcttcgc acacggcatg ctctggcgcg
     6961 tgtcgcgcga cggcgtggcg ccgagctacg tcttcggcac gatccacgtc gccgatccgc
     7021 gcgtcctcga cctgcccgag ccggtcgggc gcgcgctcgg gcgctcgcgg cggctgtacg
     7081 tcgagacgca gctcggcgcg cgcgaggagg cgcgcttttt cgaggccgcg cagttcgagg
     7141 acggccggcg gctcgagccg ctgatcggcg ccgaggccta cgcgggcgtg gccgccgagc
     7201 tgcgccggcg aaacgttccc gaggaggtca tcgcgcgcat caagccgtgg gccgcgctcg
     7261 tcaacctcac ggtgcagccc gaggactacg acggcgccac cctggatcag agaatcgcgg
     7321 cgatcgcgcg cgcgcacggc gcgcgcgtgc tcggcctgga ggggatcgag gagcagatcg
     7381 cggtcttcga gcacataccg ctcgacacgc agatcgcgct gctcaagcac gcgctcgcgc
     7441 atcgcgacga gctcgccgcg atgatcgagc cgacgatcca ggcctggatg cggcgcgacc
     7501 tcgccgggct gcacgccgcg agcgagcgcg tcgaggagcg ctacccggag atggcgccgc
     7561 actaccgcat cctgttccgg agcgtggtcg acaaccgcac cgtcgtgatg gcgcaccggc
     7621 tcttcatgcc gctgcgcgaa gggcgcgcct tcgtcgcggt cggcgccaac cacctctacg
     7681 gcgagcacgg gctgctcgcg ctgatcgcgg agcaggggta ccgcgtcacg cgcgtctact
     7741 gacgcgcgtc ccggccggcg ctagcgcgat cgagcgccgg cgggccgaag cagctgccac
     7801 accaccgcgg cgaccaggcc ggccgcgagc aggacggcgg cggtggactt cggtcgcgtc
     7861 accgcgcgcg tgtagacgct cgagctgtac acgtgactct cctggccctg gcgctcctgc
     7921 agcgcggtgc cgcccgattg gtgcagcccg tcgtcgccga cggcgcgcgc cggccgcgcg
     7981 gtgagctggc gcttgaacat gaagctctgc aggaacttgt ccatcatgcg cggcgcgtag
     8041 tgcgcggccg aggagaagaa cttcgcggcc ccgccgacga agatgtcgcg cttcgggtac
     8101 tgcgaggcga tcaggatcgc gtcggcgacc agctcgggcg cgtagatcgg tccgggcagc
     8161 ttgggctcga cctccatgaa gttcttcgcg tgcgcgacgt acatggtgtc gaccgcggcc
     8221 ggcttgatca gcgtcaccga caccggcgcc ttctcctgct ccagctccag gcgcagcgag
     8281 tccgtgaagc ccttgacggc gtgcttcgac gccgagtaga tgccctgcag cgggatggcg
     8341 acgtcggaca cttcgctgcc caggttgatg agcgcgccgc cgtgctgctt gaggtgctcg
     8401 accgccacga tcgagccgta cacgacgccc cagaagttgg tctcgaagag cttgcgctga
     8461 tcctcgacag ggatctgctc gatgcgcccg tagatcgaca cgccggcgtt gttgatccag
     8521 gtgtcgaagc ggtcgaagcg cttcagcgcc tgcgcggcga tgtcgcgcac gtccttctcc
     8581 ttgccgacgt cggcgacgac gtagatcgac tggcagccgt agcacttggc gagctcgccc
     8641 gtgagctgct tcagcgcctt ctcgttgcgc gaggcgagga cgagcttcgc gccgcgcttg
     8701 gcggcatagc gcgcggtcgt caggccgatg ccgctggagg cgccggtgat gacgatgacc
     8761 tggtcctgga gccgcttgag cggatatttc atcttttggg tcccttttgg tttcgtgaga
     8821 acattggaca cggccgcggc cgcatctccc tggaccgcaa ggaataccgg ggaagcggcc
     8881 cgcaggcaat ggaccgaaag aaggattccc ggatgtcgtg gctcatgtcg gcgctatacg
     8941 atcggttcat gcggcagagc gaggccgcct gtctcgggcg ctggcgcgcc gagctgctgc
     9001 acgacctttc tggcagcgtg ctcgagatcg gcgcgggcac gggcgccagc ctgacgcatt
     9061 atccggcgag cgtgagtcgg ctcgtgctgt gcgagccgga cccgcacatg cgccggaagc
     9121 tgcaggcgcg cgccgcggca gtggcgccgg gccgcaccga ggtcgccgat tgctccgcgc
     9181 aggcgctcgg cttcccgtcc gcctcgttcg atgccgtggt ctcgtcgctc gtgctgtgct
     9241 cggtctcgga tccggacgcg gccctcgccg aggtcgcccg cgtgctcaag ccccgcggcc
     9301 gcctggtgtt cctcgagcac gtcgccgccg atagcaatcc gccccggctc aagtggcaga
     9361 agcgcatcga gcccgtgtgg aagcacctca tgggcaactg ccacctcacg cgccgcaccg
     9421 aagccgtcat cggtgccgcc gggttccgga tcgagcgcat ccagcgcgag agcatccgca
     9481 aggcgatgcc gctggtgcgg cccagcatcc gcggcatcgc cgttaaaatg acggcgtgaa
     9541 gtccatcgtc gtcgtcatct ccggccgcgg cagcaacatg gaggccctgg tccgcgccga
     9601 ccttccggcg cggatcgcgg ccgtcgtcag caaccggccg gaagcggccg gcttgcgctt
     9661 cgcggccgag cgcggcatcc cgaccgcggt cgtcgaccac gagcgctacc cgagccgcga
     9721 cgccttcgac gccgcgctcg ccgaggcgct cgagcgccac cggcccgacc tcgtggcgct
     9781 cgcgggcttc atgcgggtgc tcggcggcga cgtcgtgagg cggtacgagg gccgcatgct
     9841 gaacatccat ccgtcgctcc ttccctcctt ccagggcctg cacacgcacg cgcgcgcgct
     9901 cgagcaggga gtgaaggtgc acggctgcac cgttcacttc gtgacgccgg cgctcgacca
     9961 cggccccatc gtgatccagg ccgccgtgcc cgtgcacccg ggcgacgacg agacggcgct
    10021 cgcggcgcgc gtcctgcgcc aggagcacgt gatctatccg cgcgcggtgc gctggttcct
    10081 cgagggacgg ctcgtcatcg aggagggccg cgtgcgcgtt accggaagcg acgaacgaca
    10141 gctcctgtgg tccgacgaat gaggcatcgc tggctggtcc tcgcggcgct cgcgccgacc
    10201 gcgctgtgcg cggcgccccc gcatcacgtg gtggtcgaat acgacatgag ccgcaacgac
    10261 atggtcatgg cgcagctcaa ggagacgctc gatcacgacg gcaagtcgta ccgcatcgtc
    10321 tcggagggca aggccacggg catcttcgcg gtgctcgcgc ccgggacggt gctgcgaacg
    10381 agcgaggggg ccgtcgcggc cgaagggctg ctgcctcgcg aataccgcga gcagcgcgga
    10441 agcaaatcgg cctccgccag gttcgaccgc tcggcgcata cgctggtgca gcagagggag
    10501 ggacaggcgg ccgagacgca gcctctcccc gagcgcgcgc aggaccgact cgcctacctg
    10561 tggagcttcg cgttcgtgcc gccgaagtcc gggcacgtcg aggccatggt cgccgacggg
    10621 cgcggcgcgc cggtgcgcta cgactacgcc gtcgcgggcg gcgagacgct caagaccccg
    10681 atgggggatc tccagacgct gcatctcgtc aagcagcgaa cgcccgacga ttcgcgccag
    10741 accgaggtct ggctggacga gaagcgcaat ttcgtgcccg tgcgggtgct cgtcgtcgag
    10801 aaggacggca cgcgcctcga ccaggtcgtc acccgaatcg aggaatgacg cgcgccatgg
    10861 ccgttccgtc ggcggtggaa acggcgcgcg cggagtcctc gcggcgctat tcgcgcgcgc
    10921 tgctcgacgc gtgctgcggc gcgctggcgg ccgtgcttcc gctcgagcgc gcggccgacg
    10981 cttcgctctc ggcgtacttc cgggatcacc cggcgctggg ccagcgcgac cgcgccctgg
    11041 tggccgacac cgtctacgcg gtgctgcggc ggcggcgcct cctcgagcgc gtcgccggcg
    11101 cgaccggcgc tcgccgcctg gtgctcgccg cctgggcggt cctgctcggc gccaacctgc
    11161 gcgagctcga gccgctcgcg cgcggcgacg agcccgggtg gctgcgcgag gtcaagcgcg
    11221 aggcccacgc gccgcagccg ttcgaggtcg agtgcgagct gcccgactgg ctgatcgagc
    11281 gcctgcggac gcgcttcgac gacgcaggcc tgctcgagat cgcacgcggg ctgctggcgc
    11341 cggcgccgct cgacctgcgg gtcaacgtcg ccaaggcgtc gcgggagcag gtgctcgagc
    11401 gcctgcgcgc cgagggcatg ggcgccgagc cgacaccgtt ttccccggac ggcatgcgcg
    11461 tcgcgggtca tcccgcgatc aaccgccacc cgctgttcgc cgacggagcg gtcgaggtgc
    11521 aggacgaggg cagccagatc gtctgccgcc tgctcgcgcc ccggcgcggc gagatggtcg
    11581 tggacttctg cgccggggcg ggcggcaaga cgctcgcgct cggggcgctc atgcgctcga
    11641 gcgggcggct gtacgcgttc gacgtgtcgg atgcgcggct cgcgaagctc aagccgcggc
    11701 tcgcccgctc gggactgtcc aacgtgcacc cgcagcgcct cgcgggcgag aacgatgcgc
    11761 gcgtcaagcg cctcgccggg aagatcgacc gcgtcctggt cgacgcgccg tgcaccgggc
    11821 tcgggaccct gcggcgcaac ccggatctca agtggcggca gacgccgggc gacgtcgtcg
    11881 agctcgccgc gaagcagcgc gccattctcg agtcggcggc gcgcctggtg aagccgggcg
    11941 ggcggctggt ctacgccacg tgcagcatcc tcgacgagga ggaccgcggc gtcgtcgagc
    12001 ctgtcctcga gcgccacccg gagttcgcgc tgcggccggc gggcgaggcg ctcgcgcagg
    12061 agcgcatcgc gatcgaggcc gaccgcttcc tcgagctctg gccgcaccgg cacgggaccg
    12121 acggcttctt cgccgcggtg ctcgagcgct ccgcatgagg aacctcgtct acgaggcgtg
    12181 gctcgacctg cagcatcccg aggtggtctg gcagatcggg gcgctcgccc tcgcgctcgc
    12241 ggtctcgtgg ctcgtcaacc ggctcgcgcg cttcgaccgc atcgaggcga gcggcgtctg
    12301 gaagttcggc gtcggcgggc tgaagcgcat cctcgcgccg gtcgtggcgc tgctgatcgt
    12361 gatcgtcgct cgcgaggtgc tgatccggca cgtgcgggtg agcgtcgacc tccttcacct
    12421 cgccgtgccg ctgctcggct cgctcgcgct ggtgcgcgtc ttcttctatg ccttgcggca
    12481 cgtgttcgcg ccgggcggcg tgctcggccg gctcgagcgc acgcttgcgg cgatcgtctg
    12541 ggtcgtgttc gcgctctaca tcgtcggcgc gctgccgacc gtcgtcgcgg gcctcgacga
    12601 cgccggcttc accctgggca ggtaccgcat ctcggcgtgg ctcgtgctcc aggccgcgtt
    12661 ctggaccttc gtcacgctgc tcggggcgct gtggtcgggc agcgcgatcg aggcgcgcct
    12721 gatgcgcgcc gaaggcctgc actacagcct gcgcgtagcg ctgtcgcgcg tgtcccgggc
    12781 gctgctggtg ctgatcgcgc tgctggtggt gctgcctctc gtcggcatcg acatcacggt
    12841 gctgtcggtc ttcggcggcg cgctcggcgt cgggcttggc ttcgggctgc agaaggtggc
    12901 gggcaactac gtcagcggct tcgtcgtcct gctcgagcgc tcgatccgga tcggcgacat
    12961 gatcacggcc gacaacttcc acggcaaggt gctcgacatc acctcgcgct acaccctggt
    13021 gcgggcgcag gacggccgcg aggcgatcat cccgaacgag acgctgatca cctcgaccgt
    13081 gctcagccat acgcacagcg accgcatgct gcgcgtcgcg tgcccggtga gcgtcgcgta
    13141 tggaaccgac ctcgaccgcg cgatgtcgac tctccgtgac gtcgcgctcg cgcatccccg
    13201 ggtgctgcgc gatcccgagc cgggcgcgct ggtgctgcgc ttcggggaca gcgggatcga
    13261 gctcgagctc gggttctgga tcggggaccc ggagcagggc acgtcgaacg tgcgctcgga
    13321 gatcaatctc gcgatctggc ggggtttgcg cgatgccggc atagagattc cctatccccg
    13381 cagagtgatc cagatcacgg gcggaaattc cgaggcaaga tcttgattcc ggtaaaaata
    13441 ccctcaggac tggagccagg aaatccccgc ttgccctttc accgaattaa cgtagaatcc
    13501 ggcgtcaaat gacgactatt cctgccgatt cctttttctt ttccggggtc ttcgaccttc
    13561 cctggtgggg cctggtgctg gtggcgctcg ggctgaccca cgtgacgatc gcctcggtga
    13621 cgatcttcct gcaccgctgc caggcgcacc gggcgctcga gctgcatccc atccccagcc
    13681 acttcttccg cttctggctg tggctgacca cgggcatggt gaccaaggag tgggcggcga
    13741 tccaccgcaa gcaccacgcc aagtgcgaga ccgtcgacga tccgcacagc ccgcaggtgt
    13801 acggcatcaa ccgcgtgctc tggggcggcg tcttcctgta cgtcagggag tcgtacaaca
    13861 aggaaacgat ggagcgctac ggtcacggga cgcccgacga ctggctcgag cgcaagatct
    13921 agtcgcgctt cgcggtgctc ggcctcacga tcatgggcgc gaccgatctg gtgctgttcg
    13981 ggccggtgcc gggcgcgctg atcctgctca cgcagatcgc ctggatcccg ttctgggcgg
    14041 cgggcgtcat caacggcatc ggtcatttct ggggctatcg caactggggg accgaggacg
    14101 cgagcaccaa tatcgtgccc tggggcatcc tgatcggcgg cgaggagctg cacaacaacc
    14161 accacgcttt cgcctcgtcg gccaagctgt cgaacaagtg gtacgagctc gacgtcggct
    14221 ggctgtacat ccgcatgctc gagagcctcg gcctcgccca ggtgaagaag gtggcgccga
    14281 cgccccggtt cgccgaggcg aagcacacgg tcgacgtcga cacgctgcag gcggtcatca
    14341 cgcaccgcta cgatgtgctg gcgaagtacg cgaagtcgct gcgcgcgacg tacgccgagg
    14401 aaatgaagaa gctgagcgcc ctggcgccgc acgacgtgca cctgttccgc ggcctcaagc
    14461 gctggctgca tatcgacgag aacagcctgt ccgagccgca gcgccaccat ctcgccgaaa
    14521 tgctgaagaa gtcgagcgcg ctgaacaccg tctattccat gcgcgccgag ctgcgcgccg
    14581 tctgggagcg ctcgagcgcg tcgcgcgagc agctcgtgcg gcagctgcag gactggtgcc
    14641 accgcgcgga ggcgagcggc atccggccgc tggtcgagtt ctc
//
LOCUS       DATDMW010000118         3427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1527447, whole genome shotgun sequence.
ACCESSION   DATDMW010000118 DATDMW010000000
VERSION     DATDMW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527447"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VI319_05250"
     CDS             complement(<1..230)
                     /locus_tag="VI319_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY6863286.1"
                     /translation="MPYQVDDVRIREIKELAPPSHLLREFPTSDRTEALAYNTRSAIH
                     RVLHGSDDRLVVVIGPCSMHDPKAGVEYARRLR"
     gene            complement(503..934)
                     /locus_tag="VI319_05255"
     CDS             complement(503..934)
                     /locus_tag="VI319_05255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY6863287.1"
                     /translation="MANASAFRPEVTASELNRRGVGQLPGLVGLQVLEVADGRVTGRM
                     PVRPDLLAPNGFLHAAAIIALADTLCGYGCVAHLPEGAKGFTTVELKSNFLGTLLEGA
                     LACEALAVHRGRNTQVWDATVTDEARGRRLALFRCTQMVLW"
     gene            1150..2121
                     /locus_tag="VI319_05260"
     CDS             1150..2121
                     /locus_tag="VI319_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6863288.1"
                     /translation="MTAAALSIPIAGFKDVYDVAEVDRALQELPPSANEALKTTYEKM
                     IKAGGTRLTVKPSGVPAMESLYDELPNFADVLDDVRKHIALCASSNDCMELPPVLLLG
                     EPGIGKTHFGKRLAHLLSTGFGFCSMSSMTAGWVISGASSQWKNAKPGKVFDTILNGT
                     YANPVIMVDEIDKASAESQYDPLGALYSLLERETATEFVDEFVEIPIDASAVVWIATA
                     NDESRIPEPILNRMNVYEITPPDRDGSIRIAQSIYSDIRTSHDWGKGFPERLGDAVAD
                     KVSELSPREMRRVLMNAFGNAKLAGRDEVALGDVGESRGPRKQRIGF"
     gene            complement(2316..>3427)
                     /locus_tag="VI319_05265"
     CDS             complement(2316..>3427)
                     /locus_tag="VI319_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY6863289.1"
                     /translation="VSSAIARALGAGARDRANALAAHAFALAIGMAVVFSGLMLSTGP
                     IVYRWMGGRGAVLEAALAYSAAASAAAISICMLNILANVVRGTGNMAFPAAVLVGSVL
                     AHVAVSPALIFGWGPMPALGPAGAGWGLAASFGIGSAALFAHLRSRDATVKLTFAGLP
                     RRGDLYRDLFRVGVPGMINVAINNLTVIVLTGVAGHLGRQAAIGYAMGARLEYIVIPL
                     AFGFGTALVAMVGTNWGAKQLPRARAIAWIGAATVAAVCGAVGVFFALFPGLWMGLFG
                     HDAEVMRLGTLYLRIAGPMYALSGLGMAIYFAMQGIGSVVPAVLANAGRLIVSAGGAL
                     AAVTWLDAGPLGVFTAIALGFAFYGAANAVVLSRR"
BASE COUNT          596 a         1182 c         1178 g          471 t
ORIGIN      
        1 cgcagccggc gggcatactc cacgcccgcc ttcgggtcgt gcatcgagca cgggccgatc
       61 accaccacca gccggtcgtc cgacccgtgc agcacgcggt ggatcgcgga ccgagtgttg
      121 taggcgagcg cctcggtcct gtcgctcgtc gggaactcgc gcagcaggtg cgacggcggg
      181 gcgagctcct tgatctcccg gatgcggacg tcgtccacct ggtagggcat ggttgcctcc
      241 tcgggggctt gcagctcgtg ctcggcggtc gtgctcatgt ctgcctctgt caaaaaaaaa
      301 ccgccagcgg gttcgctggc ggtttcgggg atccggtcga atgcggcctg ggctcagtcc
      361 ctccgccagc gggcgggaaa gccgaaaaaa taatacgcga agaaatggcc gcggctcatc
      421 acacccgatt caatcagaaa ccgccgcccc tggcaagcgg ggccgttggg gtaggccggc
      481 ttctaggccc gtacaacccc gctcaccaca ggaccatctg ggtgcagcgg aagagcgcga
      541 ggcgcctgcc gcgcgcctcg tcggtgaccg tcgcgtccca gacctgcgtg ttgcgcccgc
      601 gatgcaccgc gagcgcctcg cacgcgagcg cgccctcgag cagcgtgccg aggaaattgc
      661 tcttcagctc gacggtcgtg aaccccttcg cgccctcggg caggtgcgcc acgcagccgt
      721 agccgcacag cgtgtcggcg agcgcgatga tcgccgccgc gtgcaggaag ccgttcggtg
      781 cgagcaggtc gggccggacc ggcatgcggc cggtcacgcg gccgtccgcc acctcgagca
      841 cctggagtcc gaccagcccc ggcagctgtc cgacgccgcg ccggttcagc tccgaggccg
      901 tcacttcggg acgaaacgcc gacgcgtttg ccatttcgcg ggctcagcgg gagaagcgcg
      961 cactatagcg ggtcgacgcg aatgtgacca attgacgctc gcgatgtgcc gatcgggcgg
     1021 tttcgcccga cgcgcggtgt cgactcggaa accacggcgg cgttaaatgg gtctcagggg
     1081 caggcatgtc gattgcataa ggcgaggttg cgtggtaagt ttttcgggca actgtccagg
     1141 gagaagaaaa tgacggcagc tgcgctcagc atcccgatcg ccggattcaa ggatgtgtac
     1201 gacgtcgcgg aagtcgaccg cgcgctgcag gagcttccgc cttcggcgaa cgaggcgctc
     1261 aagacgacct acgagaagat gatcaaggcg ggcggaaccc gtctcacggt caagccctcg
     1321 ggcgtgccgg cgatggaatc cctgtacgac gagctcccca acttcgccga cgtgctcgac
     1381 gacgtgcgca agcacatcgc gctgtgcgcc tcgtccaacg actgcatgga gctgccgccg
     1441 gtgctgctcc tgggcgagcc gggcatcggc aagacccact tcggcaagcg cctcgcgcac
     1501 ctgctctcca cgggattcgg cttctgctcg atgagctcga tgaccgcggg ctgggtgatt
     1561 tcgggcgctt cctcgcagtg gaagaacgcg aagccgggca aggtgttcga cacgatcctg
     1621 aacggcacct acgcgaaccc ggtgatcatg gtcgacgaga tcgacaaggc gagcgccgag
     1681 agccagtacg atcctctggg cgcgctctac tcgctgctcg agcgcgagac cgcgaccgag
     1741 tttgtcgacg agttcgtcga gatcccgatc gacgcgtccg ccgtcgtctg gatcgccacc
     1801 gcgaacgacg agtcgcgcat cccggagccg atcctcaacc gcatgaacgt gtacgagatc
     1861 acgccgcccg accgcgacgg ctccatccgc atcgcgcagt cgatctacag cgacatccgc
     1921 accagccacg actggggcaa gggcttcccc gagcggctcg gcgacgcggt cgccgacaag
     1981 gtctcggagc tctcgccgcg cgagatgcgt cgcgtgctga tgaacgcgtt cggcaacgcc
     2041 aagctcgcgg ggcgcgacga ggtcgcgctc ggcgatgtcg gcgagagccg cggaccgagg
     2101 aagcagcgca tcgggttcta gggatcgcag ggtccgccgc ctccccggaa cagccgctgc
     2161 cgcagaaccc ggcccggaac cttgcgcgca tcggacgagc gaccgggcat cggtcgccgc
     2221 gccggcggcg cgggcgtagc ggcgccgcag gcgatcccgg ctgcgaagag gatcgagaag
     2281 aatgcgtagc gagtcatgtc agctcctttc gtgaatcagc gtcgcgagag cacgaccgcg
     2341 ttcgcggccc cgtagaaagc gaagccgagt gcgatcgcgg tgaaaacgcc caagggaccg
     2401 gcgtcgagcc aggtcacggc ggccagcgcg ccgccggcgc tgacgatcag tctcccggcg
     2461 ttggccagca cggccgggac caccgagcct atgccctgca tcgcgaagta aatggccatg
     2521 ccgaggcccg agagcgcgta catcgggccc gcgatccgca gatagagggt ccccagccgc
     2581 atcacttcgg catcgtggcc gaagaggccc atccacagcc cggggaagag cgcgaagaac
     2641 actcccaccg ctccgcacac cgccgcgacc gtcgcggcgc cgatccacgc gatggcgcgc
     2701 gcacgcggca gctgcttcgc gccccagttg gttccgacca tggcgaccag cgcggtgccg
     2761 aagccgaacg cgagcgggat cacgatgtac tcgaggcgcg cgcccatggc gtagccgatc
     2821 gcggcctggc ggccgagatg tccggccacg ccggtgagaa cgatgacggt gaggttgttg
     2881 atggcgacat tgatcattcc cgggacgccc acacggaaga ggtcgcggta gagatccccg
     2941 cgccgcggca gcccggcgaa cgtcagcttg accgtcgcgt cgcggcttcg cagatgggca
     3001 aagagggcgg cgctgccgat gccgaacgag gcggcgaggc cccagccggc gccggcgggc
     3061 ccgagcgccg gcatcgggcc ccagccgaaa atgagcgccg gcgacacggc cacgtgcgcg
     3121 aggacgcttc cgaccagcac cgccgcgggg aatgccatgt tgccggtgcc gcggacgacg
     3181 ttcgcgagga tgttcagcat gcagatcgag atcgcggctg cgctcgcggc cgccgaatag
     3241 gcgagcgcag cctcgagcac ggccccgcgg ccgcccatcc agcggtacac gatcggcccc
     3301 gtcgagagca tcagccccga gaacacgacc gccatcccga tcgcgagcgc gaacgcgtgc
     3361 gcggccagcg cgttcgcccg gtcgcgcgct cccgcgccga gcgcccgcgc gatcgccgag
     3421 gacaccg
//
LOCUS       DATDMW010000119         2813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1029884, whole genome shotgun sequence.
ACCESSION   DATDMW010000119 DATDMW010000000
VERSION     DATDMW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029884"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VI319_05270"
     CDS             complement(<1..1029)
                     /locus_tag="VI319_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY6863290.1"
                     /translation="MLVLSNEDVERLLPMADCIAALEPMYADYANGRALLSPRVDNIA
                     PGKLDDGYYAFKHMGGTWPARGIQALRINSDVITHPLVAGKPRRVKRPLANGRWVGLV
                     LLFSTETGALLAMFPDGVMQRMRVGAANGIAMKHLARADATTLALIGAGWQAGAQLMA
                     ALAVRAFREVRVFSPRKDSRDAFVAEARSRHPGVEIRAVDSAQAAAAGADVIAAATSS
                     MVRVIDPAWLKPGVHASCIKSQEVDREVFARCDRVVTHTSEQPKAEENVLAGTPNVPA
                     ESGRGWWNDGKTDLARFSDLGRIASGRLPGRARPEEITLFVNNVGMGLQFAAAGALVL
                     ERARQARAG"
     gene            1098..1173
                     /locus_tag="VI319_05275"
     tRNA            1098..1173
                     /locus_tag="VI319_05275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Arg,seq:cct)
     gene            1180..2376
                     /locus_tag="VI319_05280"
     CDS             1180..2376
                     /locus_tag="VI319_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6863291.1"
                     /translation="MTDRSNTRLPRTVYALGLVSFLNDVASDIVIPLIPIVLATVLAA
                     GPVALGLVEGVADAVASFLRLWAGRHSDVTLGRRKPLVIAGYALSNLARPLLALAGGW
                     VTVLILRSIDRVGKGIRSAPRDAWVADVAPRELTGKAFGVHRAFDNAGAVGGGLLAAA
                     AIAYAGTSLHNDILYSAIPGVLCLLLIAIVRDALQRSAPEKLPPLRWSVLSGGMRRYL
                     LVLGAFTFARVSETFLVLYGHGLGMSVVELLLLWSALNAMKSAGAYAGGVVSDRVGRK
                     AVMLTSWIGFAAAYYFVCATSTAGGLWTVTLFYGALEGLSEGAERALIKEFGREGEQG
                     TAFGWYHLLVGIAAIPAGAIFGALWQLESAAIAFSFAGAVAAASALLLAFWVKPRASR
                     ATIPGS"
     gene            2373..2501
                     /locus_tag="VI319_05285"
     CDS             2373..2501
                     /locus_tag="VI319_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863292.1"
                     /translation="MTRSQKILFALGALVFLALVAVAFRGYFTPAMLVDFVVRLCS"
     gene            2492..>2813
                     /locus_tag="VI319_05290"
     CDS             2492..>2813
                     /locus_tag="VI319_05290"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY6863293.1"
                     /translation="MQLTRFPEVLDFWFGAPDSPVRGRARKAWFTKSDEFDREIRERF
                     SSLWKKASDGALASWRGTPLAALALVVVLDQFPRNMFRGAPRAFASDALALATATDMI
                     ERRFD"
BASE COUNT          344 a         1015 c          983 g          471 t
ORIGIN      
        1 tccggcccgc gcctggcgcg cgcgctcgag caccagcgcg ccggcggccg cgaactgcag
       61 gcccatcccg acgttgttga cgaacagggt gatctcctcg gggcgggcgc gtcccggcag
      121 ccggcccgag gcgatccgcc ccaggtccga gaagcgcgcg aggtcggttt tgccgtcgtt
      181 ccaccagccg cggccgcttt ccgccggcac gttcggcgtt ccggcgagga cgttctcctc
      241 ggccttcggc tgctcggagg tgtgcgtcac gacccggtcg cagcgcgcga acacctcgcg
      301 gtcgacctcc tgcgacttga tgcagctcgc gtggacgcca ggcttcagcc acgccggatc
      361 gatcacgcgc accatcgacg acgtcgccgc cgcgatcacg tccgcgccgg ccgcggccgc
      421 ctgcgccgaa tcgaccgcgc ggatctcgac gcccgggtga cggctccgcg cctcggcgac
      481 gaaggcgtcg cggctgtcct tgcgcggact gaacacgcgc acctcgcgga acgcgcgcac
      541 cgcgagcgcc gccatcagct gcgcccccgc ctgccatccg gcgccgatca gcgcgagcgt
      601 cgtcgcgtcc gcgcgcgcga ggtgcttcat cgcgatcccg ttcgccgccc cgacgcgcat
      661 ccgctgcatc accccgtccg ggaacatcgc gagcagcgcg cccgtctcgg tcgagaacag
      721 cagcaccagc ccgacccagc gcccgttcgc gagcggccgc ttcacgcgcc gcggcttgcc
      781 ggcgacgagc gggtgcgtga tcacgtccga gttgatgcgc agcgcctgga tcccgcgcgc
      841 gggccacgtg ccgcccatgt gcttgaacgc gtagtagccg tcgtcgagct tccccggcgc
      901 gatgttgtcc acgcgcggcg agagcagcgc gcggccgttc gcatagtcgg cgtacatcgg
      961 ctcgagcgcc gcgatgcaat cggccatcgg cagcaggcgc tcgacgtctt cgttggaaag
     1021 gaccagcatg cgggctccgg atcactgttc gtatacagac gcccgtatta tcgtccatgc
     1081 gataattccc gttcagcgcc ccggtagctc agtggataga gcggccccct cctaaggggc
     1141 aggtcggacg ttcgattcgt cttcggggcg ccactcgaca tgaccgatcg ctcaaacacg
     1201 aggcttccgc gcactgtcta cgccctcggc ctcgtcagct tcctgaacga cgtcgcgagc
     1261 gacatcgtca tcccgctgat cccgatcgtg ctcgcgacgg tgctcgcggc gggcccggtc
     1321 gctctcggcc tggtcgaggg cgtggccgac gcggtcgcca gttttctgcg actctgggcc
     1381 gggcggcatt ccgacgtgac gctcggccgg cgcaagccgc tcgtgatcgc aggctacgcg
     1441 ctctcgaacc tcgcgcggcc gctgctcgcg ctcgcgggcg ggtgggtcac cgtgctgatc
     1501 ctgcgcagca ttgaccgcgt ggggaagggc atccgcagcg cgccgcgcga cgcctgggtc
     1561 gcggacgtgg cgccgcgcga gctgacgggg aaggcgttcg gcgtgcaccg cgcgttcgat
     1621 aacgcgggcg cggtcggggg cgggctgctc gcggcggcgg cgatcgcgta cgccgggacc
     1681 agcctgcaca acgacatcct gtactcggcg atccccggcg tgctgtgcct gctgctgatc
     1741 gctatagtgc gcgacgcgct gcagcgctcg gcgccggaga agctgccgcc tttgcgctgg
     1801 agcgtgctct cgggcgggat gcggcgctat ctcctggtgc tcggcgcgtt caccttcgcg
     1861 cgcgtgtcgg agaccttcct cgtgctctac ggccacggcc tcgggatgag cgtggtcgag
     1921 ctgctgctgc tgtggtccgc gctgaacgcg atgaaatccg cgggcgcgta cgcgggcggc
     1981 gtcgtgtccg accgcgtcgg gcgcaaggcc gtgatgctga ccagctggat cggattcgcg
     2041 gccgcgtatt acttcgtctg cgccacctcg acggcaggcg ggctgtggac ggtgaccctc
     2101 ttctacggcg cgctcgaagg cctgtccgaa ggcgccgagc gcgcgctgat caaggaattc
     2161 ggccgcgagg gcgagcaggg cacggcgttc ggctggtacc accttctggt cgggatcgcc
     2221 gcgatcccgg ccggcgcgat cttcggcgcg ctgtggcagc tcgagagcgc ggcgatcgcg
     2281 ttctccttcg cgggcgcggt ggcggcggcc tcggcgctgc tgctcgcctt ctgggtgaag
     2341 ccgcgggcgt cccgcgctac aatcccggga tcatgacgcg ctcgcagaag atcctcttcg
     2401 cgctcggcgc gctcgtcttc ctcgcgctcg tcgccgtcgc cttccggggc tatttcacgc
     2461 cggcgatgct ggtcgacttc gtcgtccgcc tttgcagctg acgcgcttcc ccgaggtcct
     2521 cgatttctgg ttcggcgcgc cggactcgcc ggtgcgaggc cgcgcgcgca aggcgtggtt
     2581 caccaagtcg gacgagttcg atcgcgaaat ccgcgagcgg ttctcgtcac tctggaagaa
     2641 ggcctcggac ggcgctttgg cgtcgtggcg cgggacgccg ctcgcggcgc ttgcgctcgt
     2701 ggtcgtcctc gaccagtttc cccgcaacat gttccgcggc gcgccgcgcg cgttcgcctc
     2761 cgacgcgctt gcgctggcga cggcgacgga catgatcgag cggcgtttcg acc
//
LOCUS       DATDMW010000120         6147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2024915, whole genome shotgun sequence.
ACCESSION   DATDMW010000120 DATDMW010000000
VERSION     DATDMW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024915"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VI319_05295"
     CDS             complement(<1..483)
                     /locus_tag="VI319_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6863294.1"
                     /translation="MPSWLPFEWIAASRFLREGRMQTISILLGVSIGVGVIVFMSAAL
                     AAQQANIIHRVLSAQAHISLLPPKEVARPLRGDVAEAAIVQKPFQRVRPIDQWQRILQ
                     QVAAMPAVTAVSPMAAGSLLAVRGEATRAVTVQGVEPALYFRIVPLPDKIVAGRARLD
                     A"
     gene            complement(476..1741)
                     /locus_tag="VI319_05300"
     CDS             complement(476..1741)
                     /locus_tag="VI319_05300"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6863295.1"
                     /translation="MPTYLIPEPPDYRILDEIMRLLKPRRARVALGTLALAAAAYLAF
                     GAISIPKVELATVTRRDVVQSVVASGRVATPNRVDIGSQVVGTVAAVPVVEGQEVKAG
                     QMLIALEASEARAGAKQAELAVAQAEARLRQLRELQLPVADHALRQAQANLDNARVQH
                     ERSRRLFESGYVGKSALDDARRNVDVAQAQVDSARKQVETAQPEGSDTAVARAALEQA
                     RASLQMARSRLGYTEVRAPADGTLIARDVERGEAVQPGKALMVLAPHGETQLVLQIDE
                     RNLAKLKLGQKALASADAYPSQRFAAELVYINPGVDAQRGTVEVKLRVPEPPAYLRQD
                     MTVSVDIEIERHAGALALPADAVRDTAGATPWVLAVEYGRTVRRNVKVGLRGEGAVEI
                     VDGLLEGGRVVPAGSSVRAGQRVRAAGDA"
     gene            2125..2430
                     /locus_tag="VI319_05305"
     CDS             2125..2430
                     /locus_tag="VI319_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY6863296.1"
                     /translation="MPLTPRERALVSLGAAMGSNCISCIEYHIPAARKAGLTGAQTSE
                     AIGLADKVRQVPARKVLDTAVRLLADAPVPASADTGCGEVAPTAHASGGAAKPCGCS"
     gene            2477..2704
                     /locus_tag="VI319_05310"
     CDS             2477..2704
                     /locus_tag="VI319_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY6863297.1"
                     /translation="MKLNVGTTDRAIRIVAGLVLLSLVFILESNARWWGLVGVLPLVT
                     GLAGRCLLYVPLGIDTTESGHAAPGAPHPSS"
     gene            2805..3767
                     /locus_tag="VI319_05315"
     CDS             2805..3767
                     /locus_tag="VI319_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863298.1"
                     /translation="MRLLAAALAASLVSFCAGAQTFPSRPVTLVVPFTTGTGIDILAR
                     VIGPRLSERWKQPVVVENKPGASGNIGTDAVAKAEPDGHTLMVTVNTYTITPALFKNL
                     PYDPVTDLAPISKIALATYTFAVNPTVLPAHNMKEAIDIIRKNPGKYYFASPGNGTPH
                     HVGMELIKQRLGLDIIHVPYKGFAGAMTDLVGGQVAMMFTLVHSSLPYVRAGKIRALA
                     VTGAKRSPQYPDAPTFREQGIDFMDDVDAWYAVMAPGKASPQLIARLNRDVNAVMENA
                     TVKDQLVKQGMIPTTSTPEALAALIKADLARWAKVVADAHIKID"
     gene            complement(3764..6079)
                     /locus_tag="VI319_05320"
     CDS             complement(3764..6079)
                     /locus_tag="VI319_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6863299.1"
                     /translation="MSRNGIGQSVRRVEDPRFLTGRGRYVDDIVLPRMAHGAVVVSPH
                     AHARIRSIDATAALAAPGVLAVLTGADAKAEGLGGIPPLFMPEDMGGPKGYRTFRPLL
                     EAAKARYVGDRIAFVVAETPEQARIGAELVEVDYEPLPAAVNVEDGAKEGAPRVWDDN
                     AAGNLAFPLMMGNKDATDAAFAKAKHVVSLRLRNNRLCANSLEPRATLGDFANGAYTL
                     YTSSQNPHGVRQILAQRVFSIPETSLRVIAPDVGGGFGMKGDVYPEDGLVLWASRRLG
                     RPVKWVGTRTESMLGDNHGRDQLVDAEMALDADGKILALRAHSLHAVGAYVTNAGVVP
                     VLCALRNIPNVYVVPAFHVAAKATFTHTTPLGPYRGAGRPEAAYVIERLMDAAARKLG
                     LDPVEIRRRNYIAKFPHATGTGWTYDSGDFARLTDRGVELADWGGYAARREASQRRGR
                     LRGRSFIYYLEDSGVFNERMEIRFDAGGTVTIVAGTLSHGQGHATTYAQLVSDWLGVP
                     FESIRLVQGDTDAVPFGRGTYASRSAMLGGSALKAASDAVIEKAKPLAAHLLEAAPAD
                     LEFADGKFSVAGTDRGVPLTEVAKAFHRPVGLPPQFGVGLDASGASSHPPTFPNGCHA
                     CELEVDPETGVVTIDRYVVVDDVGRVINPMICHGQVEGALAQGIGQALMENVAFDAES
                     GQMLSASFTDYAMPRADDLPPHYELDFVEVPAKTNPLGVKGVGEAGCVGAPPAVMNAL
                     LDALAPLGVEHLDMPATPDRVWRAIGAAKKG"
BASE COUNT          903 a         2231 c         2138 g          875 t
ORIGIN      
        1 ggcgtcgaga cgcgcccgcc cggcgacgat cttgtcgggc agcgggacga tgcggaagta
       61 gagcgccggc tcgacgccct gcaccgtcac cgcgcgcgtc gcctcgccgc gcacggcgag
      121 gagcgagccc gcggccatgg gcgacacggc cgtcacggcg ggcatcgcgg cgacctgctg
      181 caggatccgc tgccactggt cgatcgggcg cacgcgctgg aacggcttct gcacgatcgc
      241 ggcctcggcg acgtcgccgc gcagcggccg cgccacctcc ttcggcggca gcagcgagat
      301 gtgcgcctgc gccgagagca cgcggtggat gatgttggcc tgctgcgcgg cgagcgccgc
      361 ggacatgaac acgatcacgc cgacgccgat cgacacgccg agcaggatcg agatcgtctg
      421 catccggcct tcgcgcagga agcggctcgc cgcgatccac tcgaacggca gccagctagg
      481 catcgcccgc cgcgcgcacc cgctggcccg ccctgacgga gctgcccgcc ggcacgacgc
      541 gcccgccttc gagcaggccg tcgacgatct cgaccgcgcc ctcgccgcgc aggccgacct
      601 tgacgttgcg gcgcacggtc ctcccgtact cgacggcgag cacccagggc gtcgcgccgg
      661 ccgtgtcgcg caccgcgtcc gcgggaagcg cgagcgcgcc cgcgtgccgc tcgatctcga
      721 tatcgaccga gacggtcatg tcctgccgca aatacgccgg cggctcgggg acgcgcagct
      781 tgacttccac cgtgccgcgc tgcgcgtcga cgccgggatt gatgtagacg agctcggcgg
      841 cgaagcgctg cgacgggtac gcgtcggccg aggcgagcgc cttctggccg agcttcagct
      901 tcgcgaggtt gcgctcgtca atctgcagca cgagctgcgt ctcgccgtgc ggcgcgagca
      961 ccatcagcgc cttgcccggc tgcaccgctt cgccgcgctc gacgtcgcgc gcgatcaggg
     1021 tgccgtccgc tggcgcgcgc acctcggtgt agcccaggcg cgagcgcgcc atctgcaggc
     1081 tggcgcgcgc ctgctcgagc gcggcgcgcg cgaccgccgt gtcgctgccc tcgggctgcg
     1141 ccgtctcgac ctgcttgcgc gcggagtcga cctgcgcctg cgcgacgtcg acgttgcgcc
     1201 gggcgtcgtc gagcgccgac ttgccgacgt agccgctctc gaagaggcgg cggctgcgct
     1261 cgtgctgcac gcgcgcgttg tcgaggttcg cctgcgcctg gcgcagcgcg tggtcggcca
     1321 ccgggagctg cagctcgcgc agctgccgca gccgcgcctc ggcctgggcg accgcgagct
     1381 ccgcctgctt cgcgccggcg cgcgcctcgc tcgcctcgag cgcgatcagc atctgtcccg
     1441 ccttcacctc ctggccttcg acgaccggca ccgcggcgac cgtgccgacg acctggctgc
     1501 cgatatcgac gcggttcggc gtggccaccc gtccgctcgc gacgaccgac tgcacgacgt
     1561 cgcggcgcgt cacggtcgcg agctcgactt tcgggatcga gatcgcaccg aaggccagat
     1621 acgcggctgc cgccagcgcc agcgtgccga gggcgacgcg cgcgcggcgc ggcttgagga
     1681 gccgcatgat ctcgtcgagg atccggtagt ccggcggctc ggggatcagg taggtcggca
     1741 tgttcaccag tctcgtccgc gcggaagaaa gccctttgat gcatatcaaa aaagccgccc
     1801 cgtcgcggag ggattgacga ggatcaacac gccaggcgcg cggcctgcca tcctggaatc
     1861 gagccttcgg gaagtcctcc cggctcggat cgtcccccgt ggggggcgga cgagcggaag
     1921 ctctctcctg cacacaggag ccaggagcgg ggggactacc cgagggcttt tgtcattcga
     1981 acgcgaggcg cggcggagtg gatcgccgcg atgcggcgcg cgatcgagag cccgaggccg
     2041 ctccccgccg gcccgtatcc tattcctcag tcgtcgcgtc taagggaatg aaagacatcc
     2101 ttggagacga ctatggacac tggcatgcct ctcaccccgc gcgagcgcgc gctcgtgtcg
     2161 ctcggcgccg caatgggcag caattgcatc tcgtgcatcg agtaccacat cccggccgcc
     2221 agaaaggcgg ggctcaccgg tgcgcagacc tcggaagcga tcggcctggc cgacaaggtc
     2281 cggcaagtgc cggcacgcaa ggtgctcgac accgccgtca gactcctcgc cgacgccccc
     2341 gttccggcgt cggccgacac ggggtgcggc gaggtcgcgc cgacggcgca tgccagcggc
     2401 ggcgccgcga agccgtgcgg ctgcagctag ctcggcattc gcaaggcgct cgccctatat
     2461 cgcaaaggag cgactcatga agctaaacgt cggaacaacg gatcgcgcga tccgcatcgt
     2521 ggccgggctg gtgctgctga gcctggtatt catactcgag agcaatgccc ggtggtgggg
     2581 tctcgtcgga gtgctgcccc tcgtgacggg gctcgcgggc cgctgcctgc tgtacgtgcc
     2641 gctcggcatc gacacgacgg agagcgggca cgcggcgccg ggagcgccgc acccgagcag
     2701 ctgagccttt ccgcgcagac gatcgggggc cgcggcgttg acacgaggcg ccgtcatggt
     2761 ctaaaaccga acgcgttcgg acccctacgc cctcgcgagg ttccatgaga ctcctggccg
     2821 cggcgctcgc cgcttctctc gtttccttct gtgccggcgc gcagacgttt ccatcgcgcc
     2881 cggtcacgct ggtcgtgccg ttcaccacgg gcaccggcat cgacatcctc gcgcgcgtga
     2941 tcggcccgag gctgtctgag cgctggaagc agccggtcgt ggtcgagaac aagcccggcg
     3001 cgagcggcaa catcggcacc gatgccgtcg ccaaggccga gcccgatggc cacacgctga
     3061 tggtcacggt caacacctac acgatcacgc cggcgctgtt caagaacctg ccctacgacc
     3121 cggtcaccga cctcgcgccg atcagcaaga tcgcgctcgc gacctacacc ttcgcggtca
     3181 acccgaccgt cctccccgcg cacaacatga aggaggcgat cgacatcatc cgcaagaacc
     3241 cgggcaagta ctacttcgcc tcgcccggga acggcacgcc gcaccacgtc ggcatggagc
     3301 tgatcaagca gcggctcggg ctcgacatca tccacgtgcc ttacaagggg ttcgccgggg
     3361 cgatgaccga cctggtcggc ggccaggtgg cgatgatgtt cacgctggtg cactcgtcgc
     3421 tgccgtacgt gcgcgcgggc aagatccgcg cgctcgccgt aacgggcgcg aagcgctcgc
     3481 cgcaataccc cgacgcgccg accttccgcg agcagggcat cgacttcatg gacgacgtcg
     3541 acgcctggta cgccgtgatg gcgccgggca aggcgtcgcc tcagctcatc gcgcgcctga
     3601 accgcgacgt gaacgcggtg atggagaacg cgacggtgaa ggaccagctc gtgaagcagg
     3661 gcatgatccc gacgaccagc acgcccgagg cgctcgccgc gctgatcaag gccgacctcg
     3721 cgcgctgggc gaaggtcgtc gccgacgctc acatcaagat cgactagcct ttcttcgccg
     3781 ccccgatcgc gcgccagacg cggtcgggcg tcgccggcat gtcgaggtgc tcgacgccga
     3841 gcggcgcgag cgcgtcgagc agcgcgttca tcaccgcggg cggcgcgccg acgcagcccg
     3901 cctcgcccac acctttgacc ccgagggggt tggtcttcgc gggaacctcg acgaagtcga
     3961 gctcgtaatg cggcggcagg tcgtcggcgc gcggcatggc gtagtcggtg aagctcgccg
     4021 agagcatctg gccgctctcg gcgtcgaagg cgacgttctc catcagcgcc tggccgatcc
     4081 cctgcgcgag cgcgccctcg acctggccgt ggcagatcat cggattgatc acgcggccga
     4141 cgtcgtcgac gacgacgtag cggtcgatgg tcaccacgcc cgtctccggg tcgacctcga
     4201 gctcgcaggc gtggcagccg ttggggaagg tcggcgggtg gctcgaggcc ccgctcgcgt
     4261 cgagaccgac gccgaactgc ggcggcagcc ccaccgggcg gtggaaggcc ttggcgactt
     4321 cggtgagcgg gacgccgcgg tcggtgccgg ccacgctgaa cttcccgtcc gcgaactcga
     4381 ggtcggccgg cgcggcctcg agcaggtgcg cggccagcgg cttcgccttc tcgatcaccg
     4441 cgtcggacgc ggccttcagc gccgagccgc cgagcatggc gctgcgcgac gcataggtgc
     4501 cgcgtccgaa cggaaccgca tcggtgtcgc cctgcaccag gcggatgctt tcgaacggca
     4561 cgccgagcca gtccgagacg agctgcgcgt aggtcgtcgc gtgcccctgc ccgtgcgaca
     4621 aggtgccggc gacgatggtg acggtgccgc ccgcgtcgaa gcggatctcc atgcgctcgt
     4681 tgaagacgcc cgagtcctcg aggtagtaga taaacgagcg cccgcgcagc cggccgcgcc
     4741 gctgcgacgc ctcccggcgc gcggcgtagc cgccccagtc ggcgagctcc acgccgcggt
     4801 cggtgagccg cgcgaaatcg cccgaatcgt aggtccagcc ggtgccggtc gcatgcggga
     4861 atttcgcgat gtagttgcgc cggcggatct ccaccgggtc gagcccgagc ttgcgcgccg
     4921 ccgcgtccat caggcgctcg atcacgtacg cggcctcggg ccgtcccgcg ccgcggtaag
     4981 gaccgagcgg cgtggtatgc gtgaacgtcg ccttcgccgc gacgtggaag gccggcacga
     5041 cgtagacgtt cgggatattg cgcagcgcgc acagcaccgg caccacgccg gcgttggtga
     5101 cgtacgcgcc gaccgcgtgc aggctgtgcg cgcgcagcgc caggatcttg ccgtcggcgt
     5161 cgagcgccat ctcggcgtcg acgagctggt cgcgcccgtg gttgtcgccg agcatcgact
     5221 cggtgcgcgt gccgacccac ttcaccggcc gcccgaggcg ccgcgacgcc cagagcacga
     5281 ggccatcctc gggatagacg tcgcccttca tgccgaagcc gccgccgaca tcgggcgcga
     5341 tcacgcgaag cgaggtctcg gggatcgaga agacgcgctg cgccaggatc tggcgcacgc
     5401 cgtgcgggtt ctgcgagctc gtatacagcg tgtacgcgcc gttcgcgaaa tcccccagcg
     5461 tcgcgcgcgg ctcgagcgag ttcgcgcaga ggcggttgtt gcgcagacgc agtgatacca
     5521 cgtgcttcgc cttcgcgaac gcggcgtcgg tcgcgtcctt gttgcccatc atgagcggaa
     5581 acgcaaggtt gcccgcggcg ttgtcgtccc agactcgcgg cgcgccctcc ttcgctccat
     5641 cctcgacgtt caccgcggcg ggcagaggct cgtaatcgac ctccaccagc tccgcgccga
     5701 tgcgcgcctg ctcgggcgtc tcggccacga cgaaggcgat gcggtcgccg acgtagcgcg
     5761 ccttcgccgc ctcgagcagc ggccggaacg tgcggtagcc cttcggcccg cccatgtcct
     5821 cgggcatgaa cagcggcggg atgccgccca ggccctcggc cttggcgtcg gcgcccgtga
     5881 gcacggcgag cacgcccggc gcggcgagcg cggccgtcgc gtcgatcgag cggatcctcg
     5941 cgtgcgcgtg cggcgagacg acgaccgcgc cgtgcgccat gcgcggaagc acgatgtcgt
     6001 cgacgtagcg gccgcgcccg gtgagaaagc gcggatcctc gacgcgccgg accgactggc
     6061 cgatgccgtt gcggctcatc gccgcaccgc cgccgcgtac tcggcctcgt cgaagccgac
     6121 cagcaccgtc gacccgatct cgatcac
//
LOCUS       DATDMW010000121         9427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_283776, whole genome shotgun sequence.
ACCESSION   DATDMW010000121 DATDMW010000000
VERSION     DATDMW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283776"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VI319_05325"
     CDS             complement(<1..251)
                     /locus_tag="VI319_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HEY6863300.1"
                     /translation="MTQREGSLEAPTRHPIAWRSPDFLDEPKLFAEMERIFEICHGCR
                     RCVNLCTTFPTLFDLVDESATMEVDGVEKPKYWNVVDQC"
     gene            complement(321..740)
                     /locus_tag="VI319_05330"
     CDS             complement(321..740)
                     /locus_tag="VI319_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEY6863301.1"
                     /translation="MQLKGSKTEGNLKAAFAGESQANRRYLYFAAKADVEGQNDVAAV
                     FRSTAEGETGHAHGHLEYLEQSGDPATGLPIGNSRSNLKAAIAGETHEYTDMYPGMAK
                     QARQEGFGEIADWFETLAKAERSHANRFQKALDALSD"
     gene            complement(872..1363)
                     /locus_tag="VI319_05335"
                     /pseudo
     CDS             complement(872..1363)
                     /locus_tag="VI319_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            1479..3176
                     /locus_tag="VI319_05340"
     CDS             1479..3176
                     /locus_tag="VI319_05340"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY6863302.1"
                     /translation="MRTSRYFLATLKEAPADAELISHNLMLRAGLIRRLAGGIYTWLP
                     MGLRVVRKVEAIVREEMNRAGALELFMPAVQPAELWQESGRWEKYGPELLRFKDRHER
                     DFVIGPTHEEVITDIARREIRSYRQLPVHFYQIQTKFRDEIRPRFGVMRGREFTMKDG
                     YSFHASYADLQREYRNMHETYTRIFERLGLRFRAVAADPGSIGGTGSHEFHVLADSGE
                     DAIAYCEGSDYAANVELAEAVAPAAPRKAPGAAMRKVPTPGKTRCEDVAALLGVPLAA
                     TVKAIAVMNEGRLALLLVRGDHTLNEIKAQKTIGEFRFAADAEIEQALGCKPGYIGPV
                     GANVKIVADRSVAAMSDFVCGANEEGFHLAGVNFGRDLAEPADVADIRNVVDGDPSPD
                     GKGALRIVRGIEVGHIFQLRTKYSEAMKATFLDEAGQQRPFEMGCYGIGVTRIVGAAI
                     EQNHDERGIIFPEPIAPFRAVLIPIGYAKSAAVRETTDAMYAQLTKAGIEVLLDDRDE
                     RPGVLFADAELTGIPYRVVIGERGLKEGVVEYQPRREKEPRKVPVEEALSLLEERGK"
     gene            3173..3802
                     /locus_tag="VI319_05345"
     CDS             3173..3802
                     /locus_tag="VI319_05345"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY6863303.1"
                     /translation="MRGPALALSVLLAAFAGEAFAGAQIYEPLAESVQAGLHAAIADK
                     PAPEHGFTNSIEAVNWLTEMSHRLERKVPSFRTRLDFLRPVHYEATLAGLDPQLVLAV
                     IQVESNFRKYAVSRAGARGYMQVMPFWVELVGRKGDNLFSLRTNLRYGCVILKHYIDI
                     ERGDLTRALARYNGSIGRREYSNAVFSAWRGNWRYDGKLAGAAAPARPG"
     gene            complement(3799..4524)
                     /gene="pyrF"
                     /locus_tag="VI319_05350"
     CDS             complement(3799..4524)
                     /gene="pyrF"
                     /locus_tag="VI319_05350"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY6863304.1"
                     /translation="MTASPDSKSPPRRERLIVALDVAGADEARRLVERLGDSASFYKV
                     GLELFMSGAAFELIGWLKQRGARVFADFKLFDVPETVAAAVRSLARHGAAFATLHGNQ
                     AIMEAAGREKGAMKILAVTALTSLDRGDLDDLGFRCDVEALVLSRARRALAAGCDGVI
                     SSGLEASALKRELGERLLVVTPGIRPVENRPADDQKRTVDAAQAFRNGADYIVVGRPI
                     RNAPDPRAAALAIQGTIAEIFPR"
     gene            complement(4508..5794)
                     /locus_tag="VI319_05355"
     CDS             complement(4508..5794)
                     /locus_tag="VI319_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEY6863305.1"
                     /translation="MRHRLRQLGRFILHVLRRFQEERCLQVASSLTYTTLLSLVPLLT
                     VALTLISAFPAFQDFTANVDDFLSENVLPEPIANVISTYIQEFTEKAARLTALGVAGL
                     AVTSFLLMYTIERAFNVIWRVRRGRGIAQRVVMYWSILTLAPALIGASLTMTTFVISR
                     SLGWMETAWVGDTALKLIPPLFTVTAFTLLYYIVPSRPVRPAHALIGGLVAGVAFEIM
                     KRGFALYVALTPTYTMVYGAFATIPVFLVWIYASWVVTVMGAVVTALLPDYRLLRGES
                     RAATGEAFAEALQVLRVLVGSQRRADTPDIRRICELTGVTRESCETVLERLSDAGWVA
                     RAAGWRWVLACDPDTVTIAHVHRRFVIDAERLAERAGEGATREVLQRIAGDVARDLDA
                     PIASLAREPDDEGLSRIGPAEARTSFPRRNRSDGKP"
     gene            5858..6460
                     /gene="wrbA"
                     /locus_tag="VI319_05360"
     CDS             5858..6460
                     /gene="wrbA"
                     /locus_tag="VI319_05360"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY6863306.1"
                     /translation="MSDILVLYYSQRGAVRELAQWVARGIDEVPGAQARLRTVPRVAA
                     ATAVAEPPVPESGAPYCELRDLEECAGIAVGSPTRFGNMAAAMKQFWDGTSSLWLKGA
                     LAGKPAAVFTSTASAHGGQEATLLSMMLPLLHHGMLIVGLPYTEPELMSTRTGGTPYG
                     ASHVAGPGSERAVDEDERRLARALGRRLAAIALKLSTPGD"
     gene            complement(6444..7979)
                     /locus_tag="VI319_05365"
     CDS             complement(6444..7979)
                     /locus_tag="VI319_05365"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY6863307.1"
                     /translation="MTDRLTIFDTTMRDGEQSPGASMTKDEKLRIARQLERLRVDVIE
                     AGFAASSPGDFEAVQAVAGLIRESVVCSLARANDRDIGRAAEAVRPARRKRIHTFIAT
                     SPIHMEKKLRMTPDQVLTEAVNAVRYARKFTDDVEFSPEDAGRSDVDFLCRVLEAVIR
                     EGAGTVNIPDTVGYTMPEQFGALIRTLRERIPNADKVVWSVHCHNDLGLAVANSLAAV
                     MNGARQVECTINGLGERAGNASLEEIVMAVRTRQDVFPCDTRIDSTQIVPASRLVSGI
                     TGFPVQPNKAIVGANAFAHEAGIHQDGVLKSRETYEIMRAQDVGWSANKLVLGKHSGR
                     TAFKQRLKELGIELDSEDATNAAFQRFKDLADKKHEIFDEDLQALVSDEALSSDAEHW
                     RLVAMRQVSGTSERPRATVVLSEAGSDRNADAEGDGPVDATFKAIESLAKSGADLLLY
                     SVNAVTQGTESQGEVTVRLSRGGRIVNGVGADTDVIVASAKAYVNALNKLHSKAQRIN
                     PQV"
     gene            complement(8186..8974)
                     /gene="pssA"
                     /locus_tag="VI319_05370"
     CDS             complement(8186..8974)
                     /gene="pssA"
                     /locus_tag="VI319_05370"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY6863308.1"
                     /translation="MADLDAHKPPLRPLLNQTIRRRGIYLLPNLFTTAALFAGFYAIV
                     QGMNQQFELAAIAIVVAMVLDGLDGRVARLTRTQSAFGAEYDSLSDMVSFGVAPALVM
                     YEWALKDLGRIGWVAAFVYCVGAALRLARFNTNLGVVDKRFFQGLPSPAAAALVAGFV
                     WLANDNKIPVSEYGMPWLATLLTVYAGITMVSNVPFYSFKDINLRRSVPFAVILLIAL
                     AFVLISVDPPIIGFLLFVAYGVSGYVYWIWRRRPRTRAAPPPPP"
     gene            complement(8978..>9427)
                     /locus_tag="VI319_05375"
     CDS             complement(8978..>9427)
                     /locus_tag="VI319_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY6863309.1"
                     /translation="VVAPADGRIVAVGAAYDPYLKRDTLKISIFMNVFNAHSNRSPVD
                     GEVRRRWYHPGTFLNASLDKAALENERNALWIHAEAGDVTCVQVAGLVARRILCYVDQ
                     GTPLHRGQRFGFIRFGSRVDVYLPKNATVKAALGDKVHAATSIIAVL"
BASE COUNT         1498 a         3220 c         3259 g         1450 t
ORIGIN      
        1 tagcactggt cgaccacgtt ccagtacttc ggcttctcga cgccgtcgac ctccatcgtc
       61 gcgctctcgt cgacgaggtc gaacagcgtc gggaaggtcg tgcacaggtt cacgcagcgg
      121 cggcagccgt ggcagatctc gaagatgcgc tccatctccg cgaacagctt cggctcgtcc
      181 aggaaatcgg gcgagcgcca cgcgatcggg tggcgcgtcg gggcttcgag gctgccttcg
      241 cgctgggtca tggggagggc acgctgccga aagagaaaag gcggaaggcg ccggccttcc
      301 gcctcttcga cacgatcggc tcagtccgac agcgcgtcga gcgccttctg gaagcggttg
      361 gcgtgggagc gctcggcttt cgccagcgtc tcgaaccagt cggcgatctc gccgaaacct
      421 tcctggcgcg cctgcttcgc catgcccgga tacatgtcgg tgtactcgtg cgtctcgccc
      481 gcgatcgcgg ctttcaggtt ggagcgagag ttgccgatcg gcaggccggt cgcggggtcg
      541 cccgactgct cgaggtactc gaggtgcccg tgcgcgtgcc cggtctcgcc ctcggcggtc
      601 gaccggaaca ccgcggcgac gtcgttctga ccctcgacgt cggccttcgc ggcgaagtag
      661 agatagcggc ggttcgcctg ggattcgccg gcgaacgcgg ccttgagatt gccctcggtt
      721 ttcgagcctt tgagctgcat tgcggtctcc ttgggggaat gggaggaaaa ctagactggt
      781 tctaatcagt ggaccatgta tagcgccgcc gctgcgggtt tgtcaaggcg accggcactc
      841 gccgcgctca gggtgatccg gccgaagcgg cctgggcgcg gctcttgggc tctcggaaca
      901 ggtagcgggc gagctcggtg agcgcctgct gatagacgtc gcgcttgaac tccacgacag
      961 catcgagcgg tatccagtaa tcgtgccagc gccaggcgtc gaactcgggc ttgggcgagg
     1021 cgcgcaggca cacgtcgcaa tcgcgcccga cgaggcgcag caggaaccag atctgcttct
     1081 ggcccttgta attgccgcgc cagtcgcgct tcagccactg cgccggcact tcatagcgca
     1141 gccagtcccg cgtgcgcccg aggatcttca cgtggggggg catgaggccg acttcctcgc
     1201 gcagctcgcg gtacatcgcc tgcaccggcg tctcgccgtg cttgatgcca ccctgcggaa
     1261 actgccagga atgctcccgc acccgcttgc cccagaacac ctcgttgcgc ccgttgcaca
     1321 ggacgatgcc gacgttcggc cgatagccgt ctcgatcgag catgttccat ccagcaaaaa
     1381 atcagtcggc ttcattgttc cacactttgc cggcgatttg aaggcgagca agtcccctgt
     1441 tccattgaag taaaatgggt cgctttgccc gcccacccat gcgcacttcc cgctattttc
     1501 tcgcgacgct caaggaagct cccgccgacg ccgagctgat cagccacaac ctgatgctgc
     1561 gcgccggtct catccgccgg ctcgcgggcg gcatctacac gtggctgccg atggggctgc
     1621 gcgtggtgcg caaggtcgaa gcgatcgtcc gcgaggaaat gaaccgcgcc ggcgccctcg
     1681 agctgttcat gcccgcggtg cagcccgccg agctgtggca ggagtccggg cgctgggaga
     1741 aatacgggcc cgagctgttg cgcttcaagg accggcacga gcgcgacttc gtcatcgggc
     1801 cgacgcacga ggaagtgatc accgatatcg cgcggcgcga gatccgcagc taccgccagc
     1861 tgccggtgca tttctaccag atccagacga agttccgcga cgagatccgg ccgcgcttcg
     1921 gcgtgatgcg cggtcgcgag ttcacgatga aggacgggta ctccttccac gcgagctacg
     1981 ccgacctgca gcgcgagtac cgcaacatgc acgagaccta cacgcgcatt ttcgagcggc
     2041 tcgggctgcg ctttcgcgcg gtggccgccg acccgggctc gatcggcggg accggatcgc
     2101 acgagttcca cgtgctcgcc gattcgggcg aggacgcgat cgcgtactgc gaaggatcgg
     2161 attacgcggc caacgtcgag ctcgccgagg ccgtggcgcc cgcggcgccg cgcaaggccc
     2221 ccggcgcggc gatgcgcaag gtgccgacgc ccggcaagac gcgctgcgag gacgtggcgg
     2281 cgctgctcgg cgtgccgctc gcggcgaccg tgaaggccat cgcggtcatg aacgaggggc
     2341 gcctcgcgct gctgctcgtg cgcggcgacc acacgctgaa cgagatcaag gcgcagaaga
     2401 cgatcggcga gttccgcttc gccgccgacg ccgagatcga gcaggcgctc gggtgcaagc
     2461 cgggctacat cggtccggtc ggcgcgaacg tcaagatcgt cgccgaccgc tcggtcgccg
     2521 ccatgagcga cttcgtgtgc ggcgcgaacg aggagggctt ccacctcgcc ggcgtgaact
     2581 tcgggcgcga tctcgccgag ccggccgacg tcgccgacat ccgcaacgtc gtcgacggcg
     2641 acccgagccc cgacggcaag ggcgcgctga gaatcgtccg cgggatcgag gtcggccaca
     2701 tcttccagct gcggaccaag tactccgagg cgatgaaggc caccttcctc gacgaggccg
     2761 ggcagcagcg cccgttcgag atgggctgct acggcatcgg cgtgacgcgc atcgtcggcg
     2821 cggcgatcga gcagaaccac gacgagcgcg gcatcatatt cccggagccg atcgcgccgt
     2881 tccgggccgt gctcatcccg atcgggtacg cgaagagtgc ggccgtgcgc gagacgaccg
     2941 atgcgatgta cgcgcagctc acgaaagccg gcatcgaagt gctgctcgac gaccgcgacg
     3001 agcggccagg cgtcctgttc gcggacgccg agctcaccgg gatcccgtac cgggtagtca
     3061 tcggcgagcg cggcctcaag gagggcgtcg tcgaatacca gccgcggcgc gagaaggagc
     3121 cgcgcaaggt gccggtcgaa gaggccctga gcctgctgga ggaacgaggg aagtgagagg
     3181 tcctgcgctg gcgctttccg tattgctcgc ggccttcgcc ggcgaagcct tcgccggcgc
     3241 gcagatctac gagccgctcg ccgagagcgt gcaggccggc ctgcacgcgg cgatcgccga
     3301 caagcccgcg cccgagcacg gcttcacgaa ctcgatcgag gcggtgaact ggctgaccga
     3361 gatgtcgcac cgcctggagc gcaaggtccc gagcttccgg acgcggctcg atttcctgcg
     3421 ccccgtccac tacgaggcca cgctcgcggg gctcgatccg cagctcgtgc tcgcggtcat
     3481 ccaggtcgag agcaacttcc gcaagtacgc cgtgtcgcgc gccggggcgc gcggctacat
     3541 gcaggtcatg ccgttctggg tggagctcgt cggccgcaag ggcgacaacc tgttcagcct
     3601 gcgcaccaac ctgcgctacg gctgcgtcat cctcaagcac tacatcgaca tcgagcgcgg
     3661 cgacctgacg cgcgcgctcg cgcgctacaa cggcagcatc ggccggcgcg agtattcgaa
     3721 cgcggtgttc tccgcgtggc gcggaaactg gcgctatgac ggcaagctcg cgggcgccgc
     3781 ggctccggcg cgcccgggct agcgcggaaa aatctccgcg atcgttccct ggatggcgag
     3841 cgccgccgcg cgcgggtcgg gcgcgttgcg gatcggacgt cccacgacga tgtagtcggc
     3901 gccgttgcgg aacgcctgcg cggcgtcgac ggtgcgcttc tggtcgtcgg cgggccggtt
     3961 ctcgaccggg cggatgcccg gcgtcacgac caggagacgc tcccccagct cgcgcttgag
     4021 cgcgctcgct tcgaggcccg acgagatcac gccgtcgcat cccgccgcga gcgcgcggcg
     4081 cgcgcgagag agcacgagcg cctcgacgtc gcagcggaac ccgaggtcgt ccaggtcgcc
     4141 gcggtcgagg ctggtcagag cggtcacggc gagtatcttc atcgcgcctt tttcccggcc
     4201 ggcggcttcc atgatcgcct ggttgccgtg cagggtggcg aaagcggccc cgtggcgcgc
     4261 gagcgagcgc accgccgccg ccaccgtctc gggaacgtcg aacagcttga agtcggcgaa
     4321 caccctggcg ccgcgctgct tgagccagcc gatgagctcg aacgcggccc cggacatgaa
     4381 cagctccagc ccgaccttgt agaaggacgc cgaatcgccg aggcgctcga ccaggcgccg
     4441 cgcctcgtcg gcgcccgcga cgtcgagcgc gacgatcagg cgctcgcgcc gcggcggcga
     4501 tttcgaatca gggcttgccg tcacttcgat ttctcctcgg gaacgaggtc cgcgcctccg
     4561 ccggaccgat gcggctcagc ccttcgtcgt cgggctcccg cgcgagcgac gcgatcggtg
     4621 cgtcgagatc gcgcgcgacg tcgcccgcga tgcgctgcag gacctcgcgc gtggcgccct
     4681 cgcccgcgcg ctcggccagc cgttccgcgt cgatcacgaa ccggcgatgc acgtgcgcga
     4741 tcgtcacggt gtccggatcg cacgcgagga cccagcgcca tcccgcggcc cgcgcgaccc
     4801 atcccgcgtc ggacaggcgc tcgagcaccg tctcgcagga ctcgcgcgta acgcccgtga
     4861 gctcgcagat gcgccggata tccggcgtgt cggcgcgccg ctgcgagccg accagcacgc
     4921 gcagcacctg cagcgcctcc gcgaacgctt ctcccgttgc ggcgcggctc tcgccgcgga
     4981 gcagccggta atcgggcagc agcgccgtga ccaccgcgcc catcacggtc accacccagg
     5041 acgcgtagat ccacaccagg aacaccggga tggtggcgaa agcgccgtac accatggtgt
     5101 acgtcggcgt gagcgcgacg tagagcgcga agccgcgctt catgatctcg aacgccacgc
     5161 ccgccacgag cccgccgatc agggcgtgcg cgggccggac gggccggctc ggcacgatgt
     5221 aatagagcag cgtgaacgcc gtgaccgtga acagcggcgg gatcagcttg agcgccgtgt
     5281 ccccgaccca ggcggtctcc atccagccga gcgaccggct gatgacgaag gtggtcatgg
     5341 tgaggctcgc gccgatcagc gcgggcgcga gcgtgaggat gctccagtac atcaccacgc
     5401 gctgcgcgat gccgcggccc ctgcgcacgc gccagatcac gttgaaggcg cgctcgatcg
     5461 tgtacatgag caggaacgac gtgaccgcga gcccggcgac gccgagcgcg gtgagccggg
     5521 ccgccttctc ggtgaactcc tggatgtagg tcgagatgac gttcgcgatc ggctcgggca
     5581 gcacgttctc ggagagaaaa tcgtccacgt tggccgtgaa atcctggaac gcggggaagg
     5641 ccgagatcag ggtcagcgcc accgtcagca gcggcacgag cgagagcagc gtcgtatagg
     5701 tcaggctcga ggcgacctga agacagcgct cctcctggaa acgccgcagg acgtggagga
     5761 tgaagcgtcc gagctgccgc agccgatggc gcatgggaaa cggccgtaaa gggcctcgcg
     5821 cctataatac cagcgcgatc ccgcctcctc ccgagccgtg tccgacatcc tcgtcctcta
     5881 ctacagccag cgcggcgccg tgcgcgagct ggcgcaatgg gtcgcgcgcg gcatcgacga
     5941 ggtgccgggc gcacaggcgc ggctgcgcac cgtgccgcgc gtggcggccg cgaccgcggt
     6001 cgccgagccg cccgtgcccg agtcgggcgc gccctactgc gagctgcgcg acctcgagga
     6061 gtgcgccggc atcgccgtgg gcagccccac ccgcttcggc aacatggcgg ccgcgatgaa
     6121 gcagttctgg gacggcacct cctcgctctg gctcaaaggc gcgctcgcgg ggaagcccgc
     6181 ggccgtgttc acctcgaccg cgagcgctca cggcggccag gaggcgacgc tgctgtcgat
     6241 gatgctgccc ctgctccacc acggcatgct gatcgtcggg ctgccttaca ccgagcccga
     6301 gctgatgagc acgcgcacgg gaggcacgcc ttacggcgcg agccacgtcg ccggaccggg
     6361 atcggagcgc gcggtcgacg aggacgagcg caggctcgcg cgcgcgctgg ggcgccgcct
     6421 cgcggcgatc gccctcaagc tttctacacc tggggattga tccgctgcgc cttgctgtgc
     6481 agcttgttca gcgcgttgac gtaggccttc gccgacgcga cgatcacgtc ggtgtcggcg
     6541 ccgacaccgt tgacgatgcg tccgcccctc gacagccgca cggtcacctc gccctgcgac
     6601 tcggtgccct gggtcacggc gttcaccgag tagagcagaa ggtccgcgcc gctcttcgcg
     6661 agcgactcga tcgccttgaa ggtggcgtcc accgggccgt cgccctcggc gtcggcgttg
     6721 cggtcgctgc cggcttccga tagcaccacc gtcgcgcgcg ggcgctcgct cgtcccgctg
     6781 acctggcgca tcgcgaccag gcgccagtgc tccgcgtccg agctgagcgc ctcgtcggaa
     6841 acgagcgcct gcaggtcctc gtcgaagatc tcgtgcttct tgtcggcaag gtccttgaag
     6901 cgctggaagg cggcgttcgt ggcgtcctcg gagtcgagct cgatcccgag ctccttgagg
     6961 cgctgcttga acgcggtgcg gcccgaatgc ttgcccagca cgagcttgtt cgccgaccag
     7021 cccacgtcct gcgcgcgcat gatctcgtag gtctcgcggc tcttcagcac gccgtcctgg
     7081 tggatgcccg cctcgtgcgc gaaggcgttg gcgccgacga tcgccttgtt cggctgcacc
     7141 gggaagccgg tgatgcccga cacgagccgc gacgccggca cgatctgcgt cgagtcgatg
     7201 cgcgtgtcgc aggggaagac gtcctggcgc gtgcgtaccg ccatgacgat ctcctcgagc
     7261 gacgcgttcc cggcgcgctc gcccagcccg ttgatggtgc actccacctg gcgcgcgccg
     7321 ttcatcaccg cggcgagcga gttcgcgacc gcgaggccga gatcgttgtg gcagtgcacg
     7381 gaccacacca ccttgtccgc gttcgggatg cgctcgcgca gcgtgcggat cagcgcgccg
     7441 aactgctcgg gcatggtata gcccaccgtg tcgggaatgt tgacggtccc ggcgccttcc
     7501 ctgatcaccg cctcgagcac gcggcagagg aaatcgacgt cggagcgccc ggcgtcctcg
     7561 ggcgagaact cgacgtcgtc ggtgaacttg cgcgcgtagc gcaccgcgtt cacggcctcg
     7621 gtgagcacct ggtcgggcgt catccgcagc ttcttctcca tgtggatggg cgaggtggcg
     7681 atgaaggtgt ggatgcgctt gcggcgtgcg ggcctgaccg cctcggcggc gcggccgatg
     7741 tcgcggtcgt tggcgcgcgc gagcgagcag acgacgctct cgcggatcag tcccgccacc
     7801 gcctgcacgg cctcgaaatc tcccgggctc gacgccgcga agccggcctc gatcacgtcg
     7861 acgcgaaggc gctccagctg gcgcgcgatg cgcagcttct cgtccttcgt catggacgcg
     7921 cccgggctct gctcgccgtc gcgcatggtg gtgtcgaaaa tggtcaatcg gtctgtcatg
     7981 gagcgcctcc gtgtcgaacc gtgaggttca ttcgcagcgc tctttcgagg ctgcggccgg
     8041 gtactgccga attgggagtg gggtatttag cgcgcgaggc gcagcagcag gcccggaagc
     8101 aggccgccga gaggcagaag cagaacgagc gtgtggactc gcatggcgcc gaatatagcg
     8161 agtttcgtgc cggggcgcaa caacgtcacg gcggcggggg cggagcggca cgggtgcgcg
     8221 ggcgccgccg ccagatccag tagacgtagc ccgacactcc gtacgcgacg aacagcagga
     8281 atccgatgat gggcgggtcg acggagatca gcacgaatgc cagcgcgatc agcaggatca
     8341 ccgcgaacgg gacgctgcgc ctcaggttga tgtccttgaa gctgtagaac gggacattgc
     8401 tcaccatggt aatgcccgcg tacaccgtga gaagcgtcgc cagccacggc atgccgtact
     8461 cgctcacggg tatcttgttg tcgttcgcga gccagacgaa ccccgcgacc agcgctgcgg
     8521 ccgccgggct gggtaatccc tggaagaagc gcttgtcgac cacgccgagg ttcgtgttga
     8581 agcgcgcgag tcgcaaggcc gcaccgacac agtagacgaa cgccgcgacc cagccgatgc
     8641 ggccgagatc cttcagcgcc cactcgtaca tcacgagcgc gggcgccacg ccgaaggaaa
     8701 ccatgtccga gagcgagtcg tactcggcgc cgaacgcgct ctgggtgcgg gtgagccgag
     8761 cgacgcgtcc gtccagtccg tcgaggacca tcgccacgac gatcgcgatg gcagccagct
     8821 cgaactgctg gttcatgccc tgcacgatgg cgtagaaccc ggcgaacagc gccgcggtcg
     8881 tgaagagatt cgggagcagg tagatcccgc gcctgcggat cgtctggttg agcagcggcc
     8941 gcagcggcgg cttgtgggcg tcgaggtccg ccacgggcta cagcaccgcg atgatgctgg
     9001 tggccgcgtg caccttgtcg ccgagcgcgg ccttgacggt cgcgttcttc ggcaggtaca
     9061 cgtcgacgcg cgagccgaag cggatgaaac cgaagcgctg gccgcggtgc agcggcgtcc
     9121 cctggtcgac atagcacagg atccgccggg cgacgaggcc cgcgacttgc acgcaggtga
     9181 cgtcgcccgc ttcggcgtga atccacaagg cgttgcgctc gttctcgagc gccgccttgt
     9241 cgagggacgc gttcaggaac gtgccaggat ggtaccagcg ccgcctcacc tcgccgtcga
     9301 ccggcgagcg gttcgagtgg gcgttgaaca cgttcatgaa gatgctgatc ttcagcgtgt
     9361 cgcgcttcag gtacggatcg taggccgcgc ccacggccac gatgcgtccg tcggcgggtg
     9421 cgaccac
//
LOCUS       DATDMW010000122         7382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_906350, whole genome shotgun sequence.
ACCESSION   DATDMW010000122 DATDMW010000000
VERSION     DATDMW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906350"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VI319_05380"
     CDS             <1..603
                     /locus_tag="VI319_05380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY6863310.1"
                     /translation="TLAGMLQFAIRPMWAVNYLAHERFRMPQLEEHVDMGGGAMSIGR
                     YFTEMLDPSMSWDDVANMVREWGGRFCLKGVMSVADARRAAEIGCAGVILSNHGGRQL
                     DGSRSPFDQLAEVADAVGDRIDVLMDGGVQRGTHVLKALSLGAKAVGLGRYYLFPLAA
                     AGEAGVERALAQMRAELERDMRLMGCSSVAQLSRENLRFR"
     gene            684..1514
                     /locus_tag="VI319_05385"
     CDS             684..1514
                     /locus_tag="VI319_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEY6863311.1"
                     /translation="MNPSTADAVADVTAELSYTVDTGVKPVNETFGPNNIRRRVSATF
                     DERPVRVRDGRPLAKTFRLDTHGFELVEHRTAVRDFFDADELKRVYYAEVEALVKRVS
                     GATRVVVFDHTLRSGDESEREARRIREPVLSVHNDYTEWSGPQRVRELLPDEAGDLLE
                     RRFAIVQVWRAIARPIESNPLAIADARSIEPRDLIACERRYPHRVGETYRLAYNARHE
                     WYYFPRMRRDEALVFKVYDSEKDGRARFTAHTSFADPTTPPGAPPRQSIEARTLAFFG
                     "
     gene            1528..1956
                     /locus_tag="VI319_05390"
     CDS             1528..1956
                     /locus_tag="VI319_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863312.1"
                     /translation="MAEIRRDDPRGRRVALAVVVVGAVAGALAIAAVEHWRPELDAWL
                     RRDPSSSSSALVLAALIAGLPSLAAAAWLWAYGARVARTAMYPAPGGALLRDTRVLTG
                     PSAVRRGRLIQALAALLWMTGVLLIAALWRLWTVLPAGPA"
     gene            1953..2207
                     /locus_tag="VI319_05395"
     CDS             1953..2207
                     /locus_tag="VI319_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863313.1"
                     /translation="MSGRLLRAAIALCAGAAVGVAYPHIELALACREPASEACVWGKA
                     YFSLTLTVSLVFLGPIAAALVYAAFAWLRRQARGREGGKP"
     gene            2204..2707
                     /locus_tag="VI319_05400"
     CDS             2204..2707
                     /locus_tag="VI319_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863314.1"
                     /translation="MKPLFAVFRVIHALIALLFAIAALMLIAVATRDGWSAFFGDWDE
                     EVSQSIIEAVGLLAAAVVALQIAETIAEEEVIREADISAPTRVRRFLSRFFVVIIVSL
                     AVEGLVATFRSIHEDIRQLVYAAASILATAALLAAWGFFVHRNRAAEELEPEAMDEAK
                     REDRKLQ"
     gene            2717..3499
                     /locus_tag="VI319_05405"
     CDS             2717..3499
                     /locus_tag="VI319_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6863315.1"
                     /translation="MENPTHDPFPAPLEAACACAGDVGVVLLHGKAGTPTGRVAELGT
                     ALKRCGFRVSVPVMPWAEDRIYSASYEDSLREIDREVRQLKREGAKLVAVAGHSLGAN
                     AAIGYGAWTRADAIVALAPAHYPDQGRMRERFAADVGRARELIAAGKGGHKQTFSDLN
                     QDVPLQVTATAEAYLSWFDPEGPAVMPRNAAAFKAATPLLVIIGAGERTARWRHYFFE
                     KAPPHPKSAFTVVSGDHVEVPDRAIAQVQEWLRRLDPGAVEH"
     gene            3523..4242
                     /locus_tag="VI319_05410"
     CDS             3523..4242
                     /locus_tag="VI319_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY6863316.1"
                     /translation="MIQTFDDPDFEDHCWKDVVPAEMLEVYSHYRRDLFVGPSPALLA
                     IDLYELAYQGGARPVAEVAREHPSSCGEHAWAAIAPTQRLFAAARAAGLPVFYSTRDC
                     RPDSAPRAIVATRRRDTPTDPEVYRIKAEFAPRPGDVVVTKMRASAFFGTPLAAHLTQ
                     LGVQTLIVCGESTSGCVRATAVEAYSHGFHVVLAEECCFDRCTISHKINLFDMHHKYA
                     DVMRVDEVIGHLERSRLRKAG"
     gene            complement(4272..5216)
                     /locus_tag="VI319_05415"
     CDS             complement(4272..5216)
                     /locus_tag="VI319_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863317.1"
                     /translation="MKYAWLLLAIAASAAHAQWPAKPIRLITPFSPGGGADTLARVIT
                     PSLSQALGEPLVVENKPGAGTVIGVEYASKAEPDGYTFLIAANSMVINAALRKTRYDP
                     VKDFAPVGMTASSAVVLAVHPSVPATNAAELIAYVRANPGKIAYGDCGTGSVMHLAGE
                     LMKLIAKIDMARVTYKGCGPALTDALAGRIPVIFNTYGNTAPQARAGKLRILGLASAT
                     RSPVDASVPTIAEAGLSGIDADIWYAVFAPAGTPDAFVRRLSAETRATLAKPDIVEKL
                     RLQYFDVRPGTPEELARTVRDDLARWSKVIRDANIKID"
     gene            complement(5213..5974)
                     /locus_tag="VI319_05420"
     CDS             complement(5213..5974)
                     /locus_tag="VI319_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY6863318.1"
                     /translation="MSEPVWNRFLTERDRRVFAASGFAERQGFGKRPAVMVVDVNYAF
                     CGDRPEPILESIKRWRASCGEDAWTGIKAIKTLLAAARARGLPVIYSTGARRDDDWDR
                     GSWTWKSSRGNERPKTPAGLDGNTIVPDIAPQPQDIVIAKHKPSVFHGTPLASFLTLL
                     GADSLIVAGTTTSGCVRATVVDAFSDNYRVAVVEEACFDRSEASHAISLCDMDAKYAD
                     VVKLAEALAWIETLPRGLFELPGGGLQALRAVKSL"
     gene            6056..6532
                     /locus_tag="VI319_05425"
     CDS             6056..6532
                     /locus_tag="VI319_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863319.1"
                     /translation="MYWWNLRAAKSELRAGAPGARALVPYLIAAASVQGLIVESSFLL
                     PAQEEPTSARVWLTAGVSVAITAGGCLYVYRRNGGAAGRSLLERLLVLGWVAGVRYTA
                     FLILALAVFFGLAVALYPEAGEAAGEWSDEAFMLASAGFYLYLGRHIRDLAALQEH"
     gene            6535..7182
                     /locus_tag="VI319_05430"
     CDS             6535..7182
                     /locus_tag="VI319_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY6863320.1"
                     /translation="MSRVVVRSFGVSLDGFSAGPRQDLQNPLGVGGPDIMGWFFPTRV
                     FQKMHGRGEGETGVDNGIAEQGFDNIGAWILGRNMFGPVRGPWPDESWKGWWGEEPPY
                     HVPTFVLTHHARAPLKMKGGTTFHFVTDGIESALKQAKAAAGARDVRIGGGAATIRQY
                     LQARLIDELHLAVRPVLMGAGEKLFEGIDARALGYETAKTVMGERAMHVFLRKKT"
     gene            7199..>7382
                     /locus_tag="VI319_05435"
     CDS             7199..>7382
                     /locus_tag="VI319_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863321.1"
                     /translation="MRRFIALAALALFASAALAQGYPSRPVTIIVPFAAGGPTDVIAR
                     TLAEPMRAVLGQPVVIE"
BASE COUNT          999 a         2566 c         2694 g         1123 t
ORIGIN      
        1 acgctcgccg gcatgctgca gttcgcgatc aggccgatgt gggcggtgaa ctacctcgcg
       61 cacgagcgct tccgcatgcc gcagctcgag gagcacgtcg acatgggcgg cggcgcgatg
      121 tcgatcggcc gctatttcac cgagatgctc gacccgtcga tgagctggga cgacgtggcg
      181 aatatggtgc gcgagtgggg cggccggttc tgcctgaagg gcgtcatgtc ggtcgccgac
      241 gcgcggcgcg cggccgagat cggctgcgcc ggcgtcatcc tctccaacca cggcgggcgc
      301 cagctcgacg gctcgcgcag ccccttcgac cagctcgccg aggtcgccga cgccgtgggc
      361 gaccgcatcg acgtcctgat ggacggcggc gtgcagcgcg ggacgcacgt gctcaaggcg
      421 ctgtcgctgg gcgcgaaggc ggtcgggctc ggccgctact atctcttccc gctcgcggcc
      481 gcgggcgagg cgggcgtcga gcgcgcgctc gcgcagatgc gcgccgagct cgagcgcgac
      541 atgcgcctga tgggttgctc gtccgtcgcg cagctgtcgc gcgagaacct gcgcttccgc
      601 tgacaggctg ctgacgcgcc gcgggcggtt gcgttccggc ggccgctgac caagaatagg
      661 cggatcgatc cgcggagcgc gccatgaacc cttccaccgc cgacgccgtc gccgacgtca
      721 ccgccgagct gagctacacc gtcgacacgg gcgtgaagcc cgtgaacgag accttcgggc
      781 cgaacaacat tcgccggcgc gtgagcgcca cgttcgacga gcggccggtg cgcgtgcgcg
      841 acgggcggcc gctcgcgaag acgttccgcc tcgacaccca cggcttcgag ctcgtcgagc
      901 accgcaccgc ggtgcgcgat ttcttcgacg ccgacgagct gaagcgcgtc tattacgccg
      961 aggtcgaggc gctggtgaag cgcgtctcgg gggccacccg ggtcgtggtc ttcgaccaca
     1021 cgctgcgctc gggcgacgag agcgagcgcg aggcgcgcag gatccgcgag ccggtgctct
     1081 cggtgcacaa cgactacacc gagtggtccg ggccgcagcg cgtgcgcgag ctgctcccgg
     1141 acgaggccgg ggacctgctc gagcgccgtt tcgccatcgt ccaggtctgg cgtgcgatcg
     1201 cgcgcccgat cgagtcgaac ccgctcgcga tcgccgacgc gcgctcgatc gagccgcgcg
     1261 acctgatcgc ctgcgagcgg cgctacccgc accgcgtcgg cgagacctat cgcctcgcgt
     1321 acaacgcgcg ccacgagtgg tactacttcc cgaggatgcg ccgcgacgag gcgctcgtct
     1381 tcaaggtcta cgattccgag aaggacggcc gcgcgcgctt caccgctcac acgtccttcg
     1441 ccgacccgac cacgcctccc ggcgcgccgc cgcgccagag catcgaggcg agaacgctgg
     1501 cgttcttcgg ctagcgggcg agggcggatg gccgagatcc gcagggacga tccgcgcggg
     1561 cggcgcgtcg cgctcgccgt cgtggtggtc ggggccgtcg ccggcgcgct cgccatcgcc
     1621 gccgtcgagc attggcggcc ggagctcgac gcctggctgc ggcgcgatcc gtcctcgtcg
     1681 tcgtcggccc tcgtgctggc ggcgctgatc gccggcctgc cctcgctcgc cgcggcggcc
     1741 tggctctggg cgtacggcgc gcgcgtggcg cgcacggcga tgtaccccgc gcccggcggc
     1801 gcgttgctgc gcgacacgcg cgtgctcacg gggccgagcg ccgtgcgtcg cgggcgcctg
     1861 atccaggcgc tggccgcgct cctctggatg accggcgtcc tgctcatcgc cgcgctgtgg
     1921 cggctctgga ccgtgctgcc cgcggggccg gcgtgagcgg ccggctgctc cgggccgcga
     1981 tcgccctgtg tgccggcgcc gcggtcggcg tcgcgtaccc gcatatcgag ctggcgctcg
     2041 cgtgccgcga gcccgcgtcc gaggcgtgcg tctggggcaa ggcctacttc tcgctcacgc
     2101 tgaccgtgag cctggtgttc ctcggcccga tcgcggccgc gctggtatat gctgcgttcg
     2161 cgtggctgcg ccgccaggcg cggggccgcg agggaggcaa gccatgaagc ccttgtttgc
     2221 ggtgttccgg gtgattcatg cgctgatcgc gctgctgttc gcgatcgcgg cgctgatgct
     2281 catcgccgtc gcgacccgcg acggctggtc ggcgttcttc ggcgactggg acgaggaagt
     2341 ctcgcagtcg atcatcgagg cggtcgggct gctcgccgcc gccgtggtcg cgctgcagat
     2401 cgccgagacc atcgccgagg aggaggtgat ccgcgaggcc gacatcagcg cgccgacgcg
     2461 ggtgcgccgg ttcctgtcgc gcttcttcgt cgtgatcatc gtgtcgctcg cggtcgaagg
     2521 gctggtcgcg accttccgct cgatccacga ggacatccgg cagctcgtct acgccgcggc
     2581 ctcgatcctg gcgaccgccg cgcttctcgc cgcctggggc tttttcgtcc atcgcaaccg
     2641 cgccgccgag gagctcgagc ccgaggcgat ggacgaggcc aagcgcgagg accgcaagct
     2701 gcaatagcat tcgcgagtgg agaacccgac gcacgatccg ttcccggccc cgctcgaggc
     2761 cgcctgcgcc tgcgccggcg acgtcggcgt ggtgctgctg cacggcaagg cggggacgcc
     2821 caccggccgg gtcgccgagc tgggcacggc gctgaagcgc tgcggcttcc gggtgtcggt
     2881 cccggtgatg ccgtgggccg aggaccgtat ctacagcgcc tcgtacgagg attcgctgcg
     2941 cgagatcgat cgcgaggtcc ggcagctcaa gcgcgagggc gccaagctcg tggccgtggc
     3001 cggccacagc ctgggcgcga acgccgcgat cggctacggc gcgtggaccc gcgccgatgc
     3061 catcgtcgcg ctcgcgcccg cgcattaccc ggaccagggc cggatgcgcg agcgcttcgc
     3121 cgccgacgtc ggccgcgcgc gcgagctcat cgccgccggc aagggcggcc acaagcagac
     3181 gttctcggac ctgaaccagg acgtgccgct gcaggtcacg gcgaccgccg aggcgtacct
     3241 ctcgtggttc gaccccgagg ggcccgcggt catgccgagg aacgccgcgg cgttcaaggc
     3301 ggccacgccg ttgctcgtga tcatcggcgc cggagagcgc accgcgcgct ggcggcatta
     3361 cttcttcgag aaggcgccgc cgcatccgaa gagcgcgttc acggtggtgt ccggggacca
     3421 cgtcgaggtg ccggaccgtg cgatcgcgca ggtgcaggag tggctgcgcc ggctcgatcc
     3481 cggggcggtc gaacattagc gatcggaaag gaccgtggcg ccatgatcca gactttcgac
     3541 gacccggatt tcgaggacca ttgctggaag gacgtcgtcc cggccgagat gctcgaggtg
     3601 tactcgcact accggcgcga cctcttcgtc ggcccgtcgc cggcgctcct cgcgatcgac
     3661 ctgtacgagc tcgcctacca gggcggcgcg cgcccggtgg cggaagtcgc gcgcgagcac
     3721 ccgagctcgt gcggcgagca cgcctgggcc gcgatcgcgc cgacgcagcg cctgttcgcg
     3781 gcggcgcgcg ccgcggggct gcccgtgttc tactcgacgc gcgactgccg tcccgacagc
     3841 gcgccgcgcg cgatcgtcgc cacgcgccgg cgcgacacgc ccaccgaccc cgaggtgtac
     3901 cggatcaagg ccgagttcgc gccgcgcccg ggcgacgtcg tggtcaccaa gatgcgcgcg
     3961 agcgcgttct tcggcacgcc gctcgccgcg cacctgacgc agctcggcgt gcagacgctg
     4021 atcgtctgcg gtgagagcac ctcgggctgc gtgcgcgcga ccgcggtcga ggcctactcg
     4081 cacggcttcc acgtcgtgct cgccgaggag tgctgcttcg accgctgcac gatctcgcac
     4141 aagatcaatc ttttcgacat gcaccacaag tacgccgacg tgatgcgcgt cgacgaggtg
     4201 atcggccatc tcgaaagaag ccgcctgaga aaagcgggct gagcgatgcg ctcgcggcct
     4261 ggcgcgctcc cctaatcgat cttgatgttc gcgtccctga tgaccttgct ccagcgcgcc
     4321 aggtcgtcgc ggacggtccg tgcgagctcc tcgggcgttc cgggacggac gtcgaagtac
     4381 tggaggcgga gcttctcgac gatatcgggc ttcgcgagcg tcgcgcgggt ctcggcgctc
     4441 aggcgccgca cgaacgcgtc cggcgtgccc gccggcgcga acaccgcgta ccagatgtcg
     4501 gcgtcgatcc cgctcaggcc ggcctcggcg atcgtgggca cgctcgcgtc gacgggagag
     4561 cgcgtcgccg acgcgaggcc gaggatgcgc agcttcccgg ctctcgcctg cggcgcggtg
     4621 ttgccgtagg tgttgaagat gaccgggatg cggccggcga gcgcgtcggt gagcgcgggt
     4681 ccgcagccct tgtaggtgac gcgcgccatg tcgatcttcg cgatcagctt catcagctcg
     4741 ccggcgaggt gcatcaccga tcccgtcccg cagtcgccgt acgcgatctt gccggggttc
     4801 gcgcgcacgt aggcgatgag ctcggccgcg ttcgtggccg gcacggaagg gtgcacggcg
     4861 aggaccaccg cgctcgaggc ggtcatgccg accggggcga aatccttcac cggatcgtag
     4921 cgcgtcttgc gcagcgcggc gttgatgacc atggagttgg ccgcgatcag gaaggtgtag
     4981 ccgtcgggct cggccttcga cgcgtactcg acgccgatga cggtgccggc gccgggcttg
     5041 ttctcgacga cgagcggctc gccgagcgcc tgcgagagag aaggcgtgat gacgcgcgcg
     5101 agcgtgtcgg cgccgccgcc gggggagaac ggggtgatca gccggatggg cttcgccggc
     5161 cattgcgcgt gcgcggccga cgcggcgatc gccaggagaa gccaggcgta cttcaaagcg
     5221 acttgacggc gcgcagcgcc tggaggcctc cgcccggcag ctcgaacagt ccgcgcggca
     5281 gcgtctcgat ccaggcgagc gcctcggcga gcttgaccac gtcggcatac ttcgcgtcca
     5341 tgtcgcacag gctgatcgcg tggctcgcct cggagcggtc gaagcaggcc tcctcgacca
     5401 ccgcgacgcg gtagttgtcg gagaatgcgt ccaccacggt cgcgcgcacg cagcccgagg
     5461 tggtggtgcc ggcgacgatc agcgagtccg cgccgagcag cgtcaggaac gacgcgagcg
     5521 gcgtgccgtg gaatacgctc ggcttgtgct tcgcgatgac gatgtcctgt ggctgcgggg
     5581 cgatgtcggg gacgatggtg ttgccgtcga ggcccgcggg cgtcttcggg cgctcgttgc
     5641 cgcggctcga tttccaggtc caggagccgc ggtcccagtc gtcgtcgcgc cgggcgccgg
     5701 tcgagtagat gacgggcagg cccctggcgc gcgccgcggc gaggagcgtc ttgatcgcct
     5761 tgatgccggt ccaggcgtcc tcgccgcacg aggcgcgcca gcgcttgatc gactcgagga
     5821 tgggctcggg ccggtcgccg cagaacgcgt aattgacgtc gaccaccatg accgcgggcc
     5881 gcttgccgaa gccctggcgc tcggcgaagc ccgacgcggc gaagacccgc cggtcgcgct
     5941 cggtcagaaa ccggttccac acgggctcgc tcatgggacg ctccgaaacg atcctcgatc
     6001 gaccgggacg atgctatgct ccctcgcaaa tcggtgtcaa tcgcagggag gggcggtgta
     6061 ctggtggaac ctgcgcgcgg cgaagagcga gctccgcgcc ggcgcgcccg gcgcgagggc
     6121 gctcgtgccg tacctgatcg ccgccgcgag cgtgcaaggc ctgatcgtcg agtcctcgtt
     6181 cctgctgccg gcgcaggagg agccgacgag cgcgcgcgtg tggctcaccg ccggtgtctc
     6241 ggtcgcgatc acggccggcg gatgcctgta cgtttacagg cgcaacggcg gcgccgcggg
     6301 aagaagcctc ctcgagcggc tgctcgtgct cggctgggtc gcgggcgtgc gctacaccgc
     6361 gttcctgatc ctcgcgctcg ccgtcttctt cgggctcgcc gtcgcgctct atcccgaggc
     6421 gggcgaggcg gcgggggaat ggagcgacga ggcgttcatg ctggcctcgg cagggtttta
     6481 tctttatctc gggcgccaca tccgtgatct ggccgccctc caggagcatt gagtatgtcc
     6541 agagtcgtcg ttcgcagctt cggcgtctcc ctcgacggtt tcagcgccgg tccgcgccag
     6601 gacctgcaga atccgctcgg cgtcgggggc cccgacatca tggggtggtt cttccccacg
     6661 cgcgtgttcc agaagatgca cggccgcggc gagggcgaga ccggcgtcga caacgggatc
     6721 gccgagcagg gcttcgacaa catcggcgcg tggatcctcg gtcgcaacat gttcgggccg
     6781 gtgcgcgggc cgtggcccga cgagagctgg aagggctggt ggggcgagga gccgccgtac
     6841 cacgtgccga ccttcgtgct cacgcaccac gcgcgcgcgc ctttgaagat gaaaggcggc
     6901 accacgttcc atttcgtcac cgacggcatc gagtcggcgc tgaagcaggc gaaggccgcc
     6961 gcgggcgcgc gcgacgtgcg tatcggcggc ggcgcggcca ccatccggca gtatctccag
     7021 gcgcggctga tcgacgagct gcacctcgcc gtgcggccgg tgctgatggg cgccggcgaa
     7081 aagctcttcg aggggatcga cgcgcgcgcg ctcggctacg agaccgcgaa gaccgtcatg
     7141 ggcgagcgcg cgatgcacgt gttcctgcgc aaaaagacat gaccaaggga gggagctaat
     7201 gaggagattc atcgctctcg ccgctctcgc gctgttcgcc tcggcggcgc tggcgcaggg
     7261 ctatccgtcg cgcccggtca cgatcatcgt gcccttcgcc gcgggcgggc ccaccgacgt
     7321 gatcgcgcgc acgctcgccg agccgatgcg cgccgtgctc gggcagccgg tcgtgatcga
     7381 ga
//
LOCUS       DATDMW010000123         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1779586, whole genome shotgun sequence.
ACCESSION   DATDMW010000123 DATDMW010000000
VERSION     DATDMW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779586"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..931)
                     /locus_tag="VI319_05440"
     CDS             complement(380..931)
                     /locus_tag="VI319_05440"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6863322.1"
                     /translation="MRFPDVRLRAVATTAAVILIGGVSFAWLGDATPPADPRAVHAEK
                     KRAEEVKARFDQGVVMLHAKQYDHALTAFHRVLELEPAIPEAHVNTGYALLGLKRYDA
                     ARDFFESATELRPGQANAYWGLAVALEALGDKPGAIGAMRTFVHLSKPDDPYVRRAQA
                     ALWEWETAVAEEREKIAKAPAKK"
     gene            complement(928..1737)
                     /gene="ccsA"
                     /locus_tag="VI319_05445"
     CDS             complement(928..1737)
                     /gene="ccsA"
                     /locus_tag="VI319_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY6863323.1"
                     /translation="MSELELLWAALLAYVAAGSIAIVGVVMQRRVERAVLALAVAGLA
                     VHALSLALRWDRLGHGPFITLFEVLSSNVFSLLLVYSIAYARVRAIRPTAAVVMPILF
                     LMMGWLLVAKADDSHLPQTYHTVWLWVHVGLGKVFLGSVLVAVGISGVVLLRAAGIGT
                     SRFAALPADEKLERLAFRFMVLGFVFETLMLVAGAIWAQNAWGRYWAWDPLETWAFMT
                     WLALAFVLHLRATTKLAPAIGSAAIVAVFVLAFLTFFGVPFVSTAPHKGAI"
     gene            complement(1751..>2573)
                     /locus_tag="VI319_05450"
     CDS             complement(1751..>2573)
                     /locus_tag="VI319_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY6863324.1"
                     /translation="NLGAAVLTNAAFRRQAPLLVFHLALLAIVLLAAAGRLTYLKGRV
                     ELSTGETFAGELTQSERGPLHASRLARAAFTNEGFTIGYSAGIRRDETRNAVAWTDAG
                     GGVGRAVIGDQQPLVLAGYRFYTSPNKGYAPVFAWQPKGGSARLGTIHLPAYPAHEYR
                     QALEWTLPGSDVSVWTMLAFDDVILDPQRPSQFRLPERHTLIVRIGDERRVMAPGERL
                     ELAQGTLVYEGLTTWMGYTVFYDWTLPWLLAACVLAVASLAWHFWRKFAAQPWDA"
BASE COUNT          439 a          901 c          884 g          349 t
ORIGIN      
        1 cattgggaca tcgctcggtt ttgggagcag tccgcgcgcg atcgatttcg ggaagaacca
       61 caaaacgagc aggcataccc cggacacaat gtacccaccc gccaaaaaag ctagcgttgg
      121 tgagtcgtat ggcggacgcc acgctaccaa cgccactgcc tcacgaatga gaaccagggc
      181 ataccagatc gcaaagagtc gtacgccgag cgccaatgtc tggtggggtg tcacttcagc
      241 cttctaacct atgccggcca cggggccggt gagcacggta cggtgagcgc ggtcgtcgcg
      301 tcaactgtca atttcttcag ggcgtcgcga aacccggatt ctactgctcg acgctgaagc
      361 gcagcccgcc cgacgcgcgt cacttcttcg cgggcgcctt cgcgatcttc tcgcgttcct
      421 ccgccaccgc cgtctcccac tcccacagcg ccgcctgcgc gcgccgcacg taggggtcgt
      481 cgggcttgga caggtgcacg aaggtgcgca tcgcgccgat cgcgcccggc ttgtcgccga
      541 gcgcttcgag cgcgacggcg aggccccagt aggcgttggc ctggcccggg cgcagctcgg
      601 tcgcgctctc gaagaagtcg cgcgcggcgt cgtagcgttt gaggccgagg agcgcgtagc
      661 cggtgttgac gtgcgcctcg gggatcgcgg gctcgagctc gaggacgcgg tggaaggcgg
      721 tgagcgcgtg gtcgtactgc ttcgcgtgca gcatgacgac gccctggtcg aagcgcgcct
      781 tgacctcctc ggcacgcttc ttctccgcgt gcacggcgcg cggatcggcg ggcggcgtgg
      841 cgtcgcccag ccacgcgaag gacacgccgc cgatcaggat caccgcggcg gtcgtcgcga
      901 cggcgcgcag gcgcacatcg ggaaatctca tatcgccccc ttgtgcggcg cggtcgacac
      961 gaacggcacg ccgaagaacg tgaggaacgc gagcacgaac accgcgacga tcgccgccga
     1021 gccgatcgcg ggcgcgagct tcgtggtcgc gcgcaggtgc aggacgaacg cgagcgcgag
     1081 ccaggtcatg aacgcccagg tctcgagcgg gtcccaggcc cagtagcggc cccaggcatt
     1141 ctgcgcccag atcgccccgg cgacgagcat cagcgtctcg aacacgaagc cgagcaccat
     1201 gaagcggaag gcgagccgct cgagcttctc gtcggcgggc agcgccgcga agcggctcgt
     1261 cccgattccc gcggcgcgca gcagcaccac gcccgagatc ccgaccgcga ccagcaccga
     1321 gccgaggaac accttgccca ggcccacgtg cacccacagc catacggtgt gataggtctg
     1381 cggcaggtgg ctgtcatcgg ccttggcgac gagcagccat cccatcatca ggaacaggat
     1441 cggcattacc accgccgcgg tcggccggat cgcgcgcacg cgcgcgtacg cgatcgagta
     1501 cacgagcagc aggctgaaga cgttcgacga cagcacctcg aacagcgtga tgaacggccc
     1561 gtggccgagc cggtcccagc gcagcgcgag cgagagcgcg tgcaccgcga gcccggcgac
     1621 ggcgagcgcg agcaccgcgc gctcgacgcg cctttgcatc acgacgccga cgatcgcgat
     1681 cgatcccgcg gcgacgtacg cgagcagcgc ggcccagagg agctcgagct cgctcatttg
     1741 cgtatacatg tcaggcgtcc cacggctgcg cggcgaattt gcgccagaag tgccacgcga
     1801 ggctcgccac ggcgagcacg cacgcggcga gcagccacgg cagcgtccag tcgtagaaga
     1861 ccgtgtagcc catccaggtg gtgaggccct cgtagacgag cgtgccctgc gcgagctcca
     1921 ggcgctcgcc gggagccatc acgcggcgct cgtcgccgat gcgcacgatg agcgtgtggc
     1981 gctcgggaag ccggaactgc gacggtctct gcggatccag gatcacatcg tcgaacgcga
     2041 gcatcgtcca cacgctcacg tcgctcccgg gcagcgtcca ttcgagcgcc tgccggtact
     2101 cgtgcgcggg atacgcgggc aggtgaatgg tcccgagccg agccgagccg cccttcggct
     2161 gccacgcgaa caccggcgca tagcccttgt tcggcgacgt gtagaagcgg tagcccgcga
     2221 gcacgagcgg ctgctggtcg ccgatcacgg cgcggcccac gccgccgccg gcgtcggtcc
     2281 aggcgacggc gttgcgcgtc tcgtcgcggc ggatcccggc gctgtagccg atcgtgaagc
     2341 cctcgttcgt gaacgccgcg cgcgcgaggc gcgacgcgtg cagcgggccg cgctccgact
     2401 gcgtcagctc gcccgcgaag gtctcgccgg tggacagctc gacacgcccc ttgagatacg
     2461 tgagccgccc ggccgccgcg agcagcacga tcgcgagcag cgccaggtgg aagacgagca
     2521 gcggcgcctg cctccggaac gcagcgttcg tgagcaccgc ggcgccgagg ttc
//
LOCUS       DATDMW010000124         5766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2152883, whole genome shotgun sequence.
ACCESSION   DATDMW010000124 DATDMW010000000
VERSION     DATDMW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152883"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VI319_05455"
     CDS             complement(<1..252)
                     /locus_tag="VI319_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEY6863325.1"
                     /translation="MNIGIPAETRPGETRVAATPETVKKLAAKHTVVVQSGAGVAASV
                     PDSEYQAAGAKIAASAQEAYASDIVLKVRGPQEAEIGMLK"
     gene            407..886
                     /locus_tag="VI319_05460"
     CDS             407..886
                     /locus_tag="VI319_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863326.1"
                     /translation="MTVKVRRVVTGHDAKGRALVKIDEVAKNVISSRPGASSCVVWTT
                     EGFPVDNSGDADTSTRQVGTTLAGGVVFRIVEFSPGVAPRNHRTDSIDCAVVMSGEID
                     MELDEGRSVHLKAGDVLVQRGTIHNWVNRGSVPCQIAFVLIDAKPVQAGGKTLNAVG"
     gene            complement(892..2394)
                     /locus_tag="VI319_05465"
     CDS             complement(892..2394)
                     /locus_tag="VI319_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6863327.1"
                     /translation="MSAPERRQSDASPAMRRVGRRAPAWVLGLSTAAAALAALFVASV
                     APARGYGAVLLLAGLAVSLALYAIVRAAVGVRASDAVALSDAQAAVAAHGERLRILHE
                     IDRAIAAEEPTEAIAAAVLPPLRALLGVARVVVNMFDLAAGEVEWLAAAGRRRTHVGP
                     GVRYSIRLMGDVEALKRGEPQVVDTRALPPGREVEALLASGVETYMAVPMIAGGELIG
                     ALSFGGERGATFPAEQVRVAREVATQLAIAIAQARLLAQVRRHAQELEERVQARTREL
                     EAANEDLQSFAYSVSHDLRAPLRHIDGFSRMLEQDHAARLDDDGRRLLSTVRKGAARM
                     GRLLDDLLAFSRYGRAAISPGAVDMTALARTVADELARGTPCTVVIDTLPPARADAAL
                     VRQVWVNLIGNAIKYSGKRAEPRVEITGRVEGDLAVYGVRDNGAGFDARYADKLFKVF
                     QRLHNEDEFSGTGVGLAIVHRIVARHGGRVWAEGRPGEGATFWFALPPAG"
     gene            complement(2402..3886)
                     /locus_tag="VI319_05470"
     CDS             complement(2402..3886)
                     /locus_tag="VI319_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY6863328.1"
                     /translation="MSARLAAALLLLAALPAHADVLRIGSKRFTESYILGEVLKRQVA
                     GATAAEHKPGLGNTGIVFNALESGAIDLYPEYTGTIAKEILKLDGRPSLDELDRTLAT
                     RGLAAGIPLGFNNTYALAMRDDEAQKLGIRSITDLSRHPDVRLGLSQEFLGRADGWPG
                     LKTAYALPQATPRGLDHGLAYEAIAAGRIDVMDVYTTDAKIERYSLRVLEDDRGYFPR
                     YDAVILYRLDVPKRFPGAWSELKKLEGRIDERTMIRMNAAAELDKRSFADAAALFFGA
                     ATPRAERRFVDVLFGPDFGRLTAQHLLLVFVSLAASIALGVPLGMVAAKFARTGQLIL
                     GAVGVIQTIPSLALLAFLIAMMGTIGTAPAIVALFLYALLPIVQSTCTGLIGIAKGMR
                     QAALALGLSTRDRVRLIEVPLAMPSILAGVKTSAVINVGTATIAAFIGAGGYGERIAS
                     GLALNDTATLLAGAIPAAALALVIQGTFEFGERRLGWMRRAGRR"
     gene            complement(3883..4674)
                     /locus_tag="VI319_05475"
     CDS             complement(3883..4674)
                     /locus_tag="VI319_05475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6863329.1"
                     /translation="MSAPLFSVIVATHERPALLERALASLAAQTFRDFETIVVSDEPS
                     EATHAAAGRGLGARGVYLRRAGTPGPGPSRNAGLRLASGEYVLFLDDDDSCEPGLLER
                     FAGCGRLDGRTVLYCDLEIVMESRASMPPRRLSASRVATGAQRPEDLLVENFIPNNCV
                     AVPRAAALRLGFDERLRKLEDWDFLLGLLQHSAFAHVDFAGPRYHRDQAGEGHRDSYA
                     PADRNQFALDHLGIYRKWPAASDAQRAARAAVLARWGMQLPAGWL"
     gene            complement(4671..>5766)
                     /locus_tag="VI319_05480"
     CDS             complement(4671..>5766)
                     /locus_tag="VI319_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6863330.1"
                     /translation="DAFSTAVRRIRQRALLIALVMLLAFCAVAIILWVGGRDVLAGRL
                     TAGDLSAFVFYAVLVAGAFGAVSEVIGDLQRAAGATERLMELLAAEPSIAAPAQPKPL
                     PTPSRGEVRFERVSFHYPSRPDSAALADFSLAVSRGERVALVGPSGAGKSTVLQLLLR
                     YYDPDSGAITLDGVRLADADPRELRRRIALVPQDPVIFAASVAENVRYGRPGASDAEV
                     RAACEAAYASEFVDRLPQGADTFLGERGVRLSGGQRQRLSIARALLADRAVLLLDEAT
                     SSLDAESERWVQMALERLMRGRTTLVVAHRLATVRSADRIVVMDHGRVIASGTHAELM
                     REEGLYARLAALQFLGGAAAAESGASRIGA"
BASE COUNT          814 a         2108 c         2114 g          730 t
ORIGIN      
        1 cttcagcatc ccgatctcgg cttcctgcgg gccgcgcacc ttgagcacga tgtcgctggc
       61 gtaggcctcc tgcgcgctcg cggcgatctt cgcgccggcg gcctggtact cggaatcggg
      121 caccgaggcg gcgacgccgg cgccggactg cacgaccacc gtgtgcttcg cggcgagctt
      181 cttgacggtc tccggcgtgg ctgcgacgcg cgtttctccg ggacgggtct cggcggggat
      241 accgatgttc actttgaggt cttcttgggt ccgggccggt cgcgtgagca ggcgatcata
      301 caacaggcgg cacgaatgtt ccgcttcccg ggcgggcgcg ctttgacgca gatcaagccg
      361 ggcacggccc tataatcgcg gctcgccaat caacgaggag agcgagatga ccgtgaaggt
      421 gaggcgcgtg gtcacgggcc acgacgccaa gggccgggcg ctggtgaaga tcgacgaggt
      481 cgcgaagaac gtgatctcga gccgccccgg cgcgtcgtcg tgcgtcgtct ggaccacgga
      541 aggattcccc gtcgacaact cgggagacgc cgacacgtcg acgcgccagg tcggcacgac
      601 gctcgcgggc ggcgtggtgt tccgcatcgt cgagttctcg cccggcgtcg cgcctcgcaa
      661 tcaccgcacg gactcgatcg actgcgccgt ggtgatgtcg ggcgagatcg acatggagct
      721 cgacgagggg cgttccgtgc acctgaaggc gggcgacgtg ctcgtgcagc gcggcaccat
      781 ccacaactgg gtgaaccgcg gcagcgtccc gtgccagatc gcgttcgtgc tgatcgacgc
      841 gaagcccgtg caggcgggcg gcaagacgct gaacgcggtc ggctgagccg atcagccggc
      901 cggcgggagg gcgaaccaga acgtcgcccc ctccccgggc cggccctcgg cccaaacgcg
      961 gccgccgtgc cgagcgacga tgcggtgcac gatggcgagc cccacgccgg tgccggagaa
     1021 ctcgtcctcg ttgtgcagcc gctggaacac cttgaacagc ttgtcggcgt agcgcgcatc
     1081 gaagccggcg ccgttgtcgc gcacgccgta gaccgcgagg tcgccctcga cccggccggt
     1141 gatctcgacg cggggttcgg cgcgcttgcc gctgtacttg atcgcgttgc cgatcaggtt
     1201 gacccagacc tggcgcacga gcgccgcgtc cgcgcgcgcg ggcggcagcg tgtcgatgac
     1261 caccgtgcac ggcgtgccgc gcgcgagctc gtcggcgaca gtgcgggcga gcgcggtcat
     1321 gtcgaccgcg cccggcgaga tcgccgctcg cccgtagcgc gagaacgcga gcaggtcgtc
     1381 gagcagccgg cccatgcgcg cggcgccttt gcgcaccgtc gagagcaggc gccggccgtc
     1441 gtcgtcgagg cgcgcggcgt ggtcctgctc gagcatgcgc gagaagccgt cgatgtggcg
     1501 caaaggcgcg cgcaggtcgt gcgacaccga gtaggcgaag gactggaggt cctcgttcgc
     1561 ggcctcgagc tcgcgcgtgc gcgcctgcac ccgctcctcg agctcctggg catgccgccg
     1621 cacctgcgcg agcagccgcg cctgcgcgat cgcgatcgcg agctgcgtcg cgacctcgcg
     1681 cgcgacgcgc acctgctccg ccggaaacgt cgcgccgcgc tctccgccga agctgagcgc
     1741 gccgatcagc tcgccgcccg cgatcatcgg caccgccatg taggtctcga cgcccgaggc
     1801 gagcagcgcc tcgacctcgc ggcccggcgg cagcgcgcgc gtgtcgacga cctgcggctc
     1861 gccgcgcttc agcgcctcga cgtcgcccat cagccggatc gagtagcgca cgccggggcc
     1921 gacgtgcgtg cgacggcggc ccgcggccgc gagccactcg acctcgcccg ccgcgagatc
     1981 gaacatgttc accaccacgc gcgcgacccc gagcagcgcg cgcagcggcg gcagcaccgc
     2041 ggcggcgatc gcctcggtcg gctcctcggc ggcgatcgcg cggtcgatct cgtgcaggat
     2101 gcgcaggcgc tcgccgtgcg cggcgaccgc ggcctgcgcg tcgctcagcg cgactgcgtc
     2161 gctcgcccgc acgccgaccg ccgcgcgcac gatcgcgtac agcgccaggc tcaccgcgag
     2221 gcccgcgagc agcaggacgg cgccgtaccc ccttgcgggc gccaccgacg cgacgaacag
     2281 cgccgcgagc gcggccgcgg ccgtcgacag cccgagcacc caggccggcg cgcgccgtcc
     2341 gacccgccgc atcgcgggcg aggcgtcgga ctgcctgcgc tcgggcgcgc tcacggacac
     2401 gttatcgccg cccggcgcgc ctcatccacc cgagccgccg ctcgccgaac tcgaacgtcc
     2461 cctggatgac gagcgccagc gccgccgccg gtatcgcgcc cgcgagcagc gtggcggtgt
     2521 cgttgagcgc gaggcccgag gcgatgcgct cgccgtagcc gcccgcgccg atgaacgcgg
     2581 cgatggtcgc ggtgccgacg ttgatcaccg cggaggtctt cacgcccgcg aggatcgaag
     2641 gcatcgcgag cggcacctcg atcagccgca cgcggtctcg cgtcgacagg ccgagcgcga
     2701 gcgccgcctg acgcatgccc ttggcgatgc cgatcaggcc ggtgcacgtg ctctgcacga
     2761 tcggcagcag cgcatagagg aagagcgcga cgatcgcggg cgccgtcccg atcgttccca
     2821 tcatcgcgat gagaaaggcg agcagcgcga gcgacgggat ggtctggatc acgccgaccg
     2881 cgccgaggat cagctgtccc gtgcgcgcga atttcgcggc gaccatgccg agcggcacgc
     2941 cgagcgcgat gctcgccgcg agcgagacga acacgagcag caggtgctgc gcggtgagcc
     3001 gcccgaagtc cgggccgaag agcacgtcga cgaagcggcg ctcggcgcgc ggcgtggcgg
     3061 cgccgaagaa gagcgccgcg gcgtcggcga agctgcgctt gtcgagctcg gccgcggcgt
     3121 tcatgcggat catggtgcgc tcgtcgatcc tgccttcgag cttcttcagc tcggaccacg
     3181 cgccgggaaa gcgcttcggc acgtccaggc ggtagaggat caccgcgtcg tagcgcggga
     3241 agtagccgcg gtcgtcctcg agcacgcgca aggagtagcg ctcgatcttg gcgtcggtcg
     3301 tgtagacgtc catgacgtcg atcctgccgg cggcgatcgc ctcgtacgcg aggccgtggt
     3361 cgaggccccg cggcgtcgcc tgcggcagcg cgtacgcggt cttcaggccc ggccagccgt
     3421 cggcgcggcc gaggaactcc tgggaaagcc cgaggcgcac gtcgggatgc cgggagagat
     3481 ccgtgatcga gcggatgccg agcttctgcg cctcgtcgtc gcgcatcgcg agcgcgtagg
     3541 tgttgttgaa gccgagcggg atcccggccg cgagcccgcg cgtcgcgagc gtgcggtcga
     3601 gctcgtcgag cgacggccgg ccgtcgagct tcaggatctc cttcgcgatc gtgccggtgt
     3661 actcgggata caggtcgatc gcgcccgact cgagcgcgtt gaagacgatg ccggtgttgc
     3721 cgaggcccgg cttgtgctcg gccgccgtcg cgcccgcgac ctggcgcttc agcacctcgc
     3781 cgagaatata ggactcggtg aagcgcttcg acccgatgcg cagcacgtcg gcgtgcgcgg
     3841 gcagcgccgc gaggagcagc agagccgcgg cgaggcgcgc gctcagagcc acccggccgg
     3901 gagctgcatg ccccatcgcg cgagcaccgc ggcgcgcgcg gcgcgctgcg cgtcgctcgc
     3961 cgccggccac ttgcggtaga tgccgaggtg gtccagcgcg aactggttgc ggtcggccgg
     4021 ggcgtaggaa tcgcggtggc cctcgcccgc ctggtcgcgg tggtagcgcg ggcctgcgaa
     4081 gtcgacgtgc gcgaaggcgc tgtgctgcag cagcccgagc aggaagtccc agtcctcgag
     4141 cttgcgcagc cgctcgtcga acccgagccg cagcgcggcg gcgcgcggca cggcgacgca
     4201 gttgttcggg atgaaattct cgaccaggag gtcctcggga cgctgcgcgc cggtggcgac
     4261 gcggctcgcc gagaggcgcc gcggcggcat gctcgcgcgc gattccatga cgatctcgag
     4321 atcgcagtag agcaccgtgc ggccgtcgag ccggccgcag ccggcgaagc gctcgagcag
     4381 cccgggctcg cagctgtcgt cgtcgtcgag gaagagcacg tactcgccgc tcgcgagccg
     4441 caggcccgcg ttgcggctcg ggccggggcc cggagtcccg gcgcgcctca aatagacgcc
     4501 gcgcgcgccg agcccgcggc ccgcggccgc gtgcgtcgcc tcgctcggct cgtcgctcac
     4561 gacgatggtc tcgaagtcgc ggaaggtctg cgccgcgagc gacgcgagcg cgcgctcgag
     4621 cagcgccggc cgctcgtgcg tggcgacgat caccgagaac aggggcgcgc tcatgcgcct
     4681 attctagacg cacccgattc ggccgcggcc gcgccgccga ggaactgcag cgccgcgagc
     4741 cgcgcgtaca gcccctcctc gcgcatcagc tcggcgtgcg tgccgctcgc gatgacgcgc
     4801 ccgtggtcca tcacgacgat ccggtcggcg ctccgcaccg tcgccagccg gtgcgcgacg
     4861 acgagcgtgg tgcgcccgcg catcaggcgc tcgagcgcca tctgcaccca gcgctcgctc
     4921 tcggcgtcga gcgagctggt cgcctcgtcg aggagcagca ccgcgcggtc ggcgagcagc
     4981 gcccgcgcga tcgacagccg ctggcgctgc ccgcccgaga gccgcacgcc gcgctcgccg
     5041 aggaaggtgt ccgcgccctg cggcagccgg tcgacgaact cgctcgcgta cgcggcctcg
     5101 cacgcggcgc gcacctcggc gtcgctcgcc ccggggcggc cgtagcgcac gttctcggcg
     5161 acgctcgcgg cgaagatcac cgggtcctgc ggcacgagcg cgatgcgccg ccgcagctcg
     5221 cgcggatcgg cgtcggcgag acgcacgccg tcgagcgtga tcgcgcccga gtccgggtcg
     5281 tagtagcgca gcagcagctg gagcacggtc gacttccccg cgcccgacgg ccccacgagc
     5341 gcgacgcgct cgccgcgcga cacggcgagc gagaaatcgg ccagcgcggc gctgtcggga
     5401 cgcgacgggt agtggaagct cacgcgctcg aagcgcacct cgccgcgcga aggcgtcggc
     5461 agcggcttcg gctgcgcggg cgccgcgatc gagggctcgg ccgcgagcag ctccatcagc
     5521 cgctcggtcg cgcccgccgc gcgctgcagg tcgccgatca cctcggacac cgcgccgaag
     5581 gcgcccgcga ccagcaccgc gtagaacacg aaggccgaca ggtcgcccgc cgtcagccgc
     5641 cccgcgagca cgtcgcgccc gccgacccag aggatgatcg cgaccgcgca gaaggcgagc
     5701 agcatcacca gagcgatcag cagcgcgcgc tgccggatgc gccgcaccgc ggtcgagaac
     5761 gcatcc
//
LOCUS       DATDMW010000125         2465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1156197, whole genome shotgun sequence.
ACCESSION   DATDMW010000125 DATDMW010000000
VERSION     DATDMW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156197"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VI319_05485"
     CDS             <1..232
                     /locus_tag="VI319_05485"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY6863331.1"
                     /translation="VILWIYSAKPRPVGAVSAAFLVGYGVFRFIAEYFREPDDFLGLL
                     ALNLSMGQWLSLPMIVAGLGLFAWAWHRRKKV"
     gene            242..460
                     /locus_tag="VI319_05490"
     CDS             242..460
                     /locus_tag="VI319_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY6863332.1"
                     /translation="MIGTTEIGLRDQLQRRLGELMVEHRDLDVAIHRLGDDPVHDELQ
                     LRRLKKRKLLLKDQIAWIERQIDPDVPA"
     gene            complement(472..>2465)
                     /locus_tag="VI319_05495"
     CDS             complement(472..>2465)
                     /locus_tag="VI319_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6863333.1"
                     /translation="RGVVERFIRGDPAAVLFTEFAGDDPADIRARLARLVELLADLGH
                     PGSVIEIVDAQLQRDVWEVRKAGLNILMSMKGDGKPVSFIEDCAVPLENLADYTERLT
                     EVFRKHGTEGTWYAHASVGTLHVRPVLNMKEDGAKKMRAIAEEACALVKSYKGAAYSG
                     EHGDGLVRSEWIAPILGARLAAALAEIKDAFDPKGLMNPGKIVRPPKQDDRSLFRYKN
                     GYATQPLATALDWSEWEVQSEVERIHGRGFAAAVEMCNNNGHCRKFDAGTMCPSFRAT
                     GDEQHLTRGRANTLRLALSGQLPGGLASEAVKAALDLCVSCKGCKRECPTGVDMARMK
                     IEFLHHWHAARGASLKDRLIAYLPRYAPWAARAPWLSNLRDAIPGLAALTEGITGLSS
                     KRRLPKWRSDSFLRSGQANTSSVSSGREVVLFVDTFNNYFEPENARAALEVLAAGGYR
                     VHVARAPDGGRPLCCGRTFLAAGMVAQAKREAERTLAALRPYLERGVPVVGLEPSCIL
                     GLRDEYLGLALGKAAGELALDSHLLEEFLVREKKAGRVDLKLKGNGAKKALLHGHCHQ
                     KAFGAMGAVEQVLKWIPGLEVETVQSSCCGMAGSFGYEAAHYEISMKMAETSLLPKVR
                     AAAADTVVVADGTSCRHQIADGAGRDAVHVARVLREALS"
BASE COUNT          352 a          868 c          832 g          413 t
ORIGIN      
        1 cgtgatcctg tggatctact cggccaagcc gcggcccgtg ggggcggtgt cggcggcatt
       61 cctggtcggc tacggggttt ttcgctttat tgccgaatac ttccgcgagc ccgacgattt
      121 cctgggcctg cttgcactga acctttccat gggtcagtgg ctctcattgc cgatgatcgt
      181 tgccgggctg ggactcttcg cctgggcttg gcatcggcga aagaaggtct aatatagcca
      241 catgatcggg acgaccgaaa tcgggctccg agaccagctg cagcggcggt tgggcgagct
      301 gatggtcgag caccgcgatc tcgacgtcgc catccaccgc ctgggcgacg atcccgtcca
      361 cgacgagctg cagctgcgtc gtctcaagaa gcgcaagctt ctcctcaaag accagatcgc
      421 ctggatcgag cggcagatcg atcccgacgt ccccgcgtag cagcccctta gctaggacag
      481 cgcttcgcgc agcacgcgcg cgacgtgcac ggcgtcgcgc cccgcgccgt cggcgatctg
      541 gtggcggcag ctcgtcccgt cggcgacgac gaccgtgtcg gccgcggccg cgcgcacctt
      601 cggcagcagc gacgtctcgg ccatcttcat cgagatctcg tagtgcgcgg cctcgtagcc
      661 gaagctgccc gccatgccgc agcacgagct ctggacggtc tcgacctcga gtcccgggat
      721 ccatttcagc acctgctcga ccgcgcccat ggcgccgaag gccttttgat ggcaatggcc
      781 gtggaggagc gcctttttcg cgccgttgcc cttcagcttc aggtcgactc gtccggcctt
      841 cttctcgcgg accaggaact cctcgagcag gtgcgagtcg agcgcgagct cgcccgcggc
      901 cttgcccagg gccagcccga ggtattcgtc gcgcagcccg aggatgcacg agggctcgag
      961 gccgaccacc ggcacgccgc gctcgaggta aggccgcagc gcggcgagcg tgcgctcggc
     1021 ttcgcgcttc gcctgcgcga ccattccggc ggcgaggaag gtgcgtccgc agcacagcgg
     1081 ccggccgccg tcgggcgcgc gcgcgacgtg cacccggtag ccgccggcgg cgagcacctc
     1141 gagcgccgcg cgcgcgttct ccggctcgaa gtaattgttg aacgtgtcga cgaacagcac
     1201 gacttcccgg ccggaggata cggaggatgt gtttgcttgc cccgaccgga ggaagctgtc
     1261 ggagcgccat ttcggcagtc tgcgcttcga cgacaggccg gtgatgcctt ccgtcagggc
     1321 cgccagcccg ggaatcgcgt cgcgcaggtt ggacagccac ggcgcccgcg cggcccaggg
     1381 cgcgtagcgc ggcaggtacg cgatcagcct gtccttcagc gacgcgccgc gcgccgcgtg
     1441 ccagtgatgc aggaactcga tcttcatccg cgccatgtcg acgccggtcg gacactcgcg
     1501 cttgcagcct ttgcacgaca cgcacagatc cagcgccgcc ttgaccgcct ccgacgcgag
     1561 cccgcccggg agctggcccg agagcgcgag gcgcagcgtg ttggcgcggc cgcgcgtgag
     1621 gtgctgctcg tcgccggtgg cgcggaacga cgggcacatc gtgcccgcgt cgaacttgcg
     1681 gcagtggccg ttgttgttgc acatctccac ggccgcggcg aacccgcggc cgtggatgcg
     1741 ctcgacctcg ctctgcacct cccactccga ccagtcgagc gcggtcgcga gcggctgcgt
     1801 cgcgtagccg ttcttgtagc ggaacaggct gcggtcgtcc tgcttcggcg ggcgcacgat
     1861 cttgcccggg ttcatcaggc ccttcgggtc gaaggcgtcc ttgatctcgg cgagcgcggc
     1921 ggcgaggcgc gcgcccagga tcggcgcgat ccactccgag cgcaccaggc cgtcgccgtg
     1981 ctcgcctgag tacgcggcgc ccttgtagct cttcacgagc gcgcacgcct cctcggcgat
     2041 cgcgcgcatc ttcttcgcgc cgtcctcctt catgttgagc accgggcgca cgtgcagcgt
     2101 gccgaccgac gcgtgcgcgt accacgtgcc ctcggtgccg tgcttgcgga agacctcggt
     2161 caggcgctcg gtgtagtcgg cgaggttctc gagcggcacg gcgcagtcct cgatgaagga
     2221 caccggcttg ccgtccccct tcatcgacat caggatgttc agccctgcct tgcgcacttc
     2281 ccagacgtcg cgctgcagct gggcgtcgac gatctcgatc acgctcccgg gatggccgag
     2341 gtcggcaagc agctcgacga gcctggcgag ccgcgcgcgg atgtcggccg ggtcgtcgcc
     2401 ggcgaactcg gtaaacagca cggccgcagg atcgccgcgg atgaagcgct ccacgacacc
     2461 gcgat
//
LOCUS       DATDMW010000126         3545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2028698, whole genome shotgun sequence.
ACCESSION   DATDMW010000126 DATDMW010000000
VERSION     DATDMW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028698"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VI319_05500"
     CDS             complement(<1..885)
                     /locus_tag="VI319_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6863334.1"
                     /translation="MRTLAAALCVLLAGCQPAQSDLALGRSVWNGSCYFCHGYSGDAR
                     TVAARYLEPKPVDFTAPAASRLTREDIARAVRDGRPGTAMRGFAGRLSPAEIAAVAAF
                     VREELIAKKSENTRYHTRENGWPDHERYRAAYPFARGQIALAVPAETLAPEQAAGRRL
                     FLGSCVSCHDGGGRKEPPVWETRAVSFPLNVDACASCHAYSAALHDRAPPADATHGGQ
                     VKREDYAAGPYAKHDVAPRLAHATPLERRGEALFRKNCAFCHAADGSGRNWIGTFMEP
                     HPRDLADPEFRRAATRERI"
     gene            complement(885..1676)
                     /locus_tag="VI319_05505"
     CDS             complement(885..1676)
                     /locus_tag="VI319_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6863335.1"
                     /translation="MRRLGIVLQVASVSAALALLGPAVSLAADAPAAKRAIKPDLIYH
                     NYCSVCHGDRGDGRSRARGSLVPPPKDFTAPGLREQLPRDWMVRVVRDGKPGTAMVGW
                     KTQLDEREIEAVVDYVRDAFMSRQPGAPAPDVSGTSAHGGRGRDPSAATAPQGMRADM
                     SLPLPNGLAGDRARGKRFYDANCATCHGVKGDAQGPRAYFINPKPRNFLDASSRAQLN
                     RPALYAAIYAGRRGTEMPAWRTVIDDQTMADVAEYVFTAFVRPAK"
     gene            1762..2877
                     /locus_tag="VI319_05510"
     CDS             1762..2877
                     /locus_tag="VI319_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEY6863336.1"
                     /translation="MHKNTPRSGIAFACLAAALLAACAQVPTGSERAASGPPVFPSPP
                     DEPRFVYERSLYSSGDVVPDEAGAAFRRMVTGEQRSGEGLAKPYAVAVYHGRVYLSDS
                     VERFIKVFDVPNGRYYKIGADDPGRLYKPLGIDIDRAGNLYVADATQKAILVYGANGK
                     YLRKIGGPAVFDRLASVTVERSGERVYAVDIGGVSSELHRVRVFDAKTGEHLFDIGKR
                     GSGAGEFNLPRDVAIGREGRLYVVDGGNFRVQVFERDGRFVRSFGSVGRQMGQFARPK
                     EIAADADGNVYVIDTAFGNFQIFGPEGDLLMYVGTRSERDGPARFMLPSGVYVDEDGR
                     VYVVDQWFRKVDVFRPYALSADSGWLGRRPGTKSPAK"
     gene            2982..>3545
                     /locus_tag="VI319_05515"
     CDS             2982..>3545
                     /locus_tag="VI319_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863337.1"
                     /translation="MQGLKQGFIRGLDRWGGWIAAAGVALFVATATQYAAAQTSGSIA
                     NTKHNLSSTAVAGQNRVTAGTDEICVFCHTPHGADTTAPAPLWNKSLSSLSGNTYKTY
                     DSLGSTTIDGAFATDGVNNSSVGSVSLACLSCHDGTQAMDNIINAPGSGGYQVNGGGA
                     TGLAYTWDTTTGRVDADGKLIANFIANI"
BASE COUNT          519 a         1255 c         1271 g          500 t
ORIGIN      
        1 gatgcgctcg cgcgtcgccg cgcgccggaa ctccgggtcg gcgaggtcgc gcggatgcgg
       61 ctccatgaac gtgccgatcc agttccggcc gctgccgtcg gcggcgtggc agaaggcaca
      121 gttcttccgg aacagcgcct cgccgcggcg ctcgagcggc gtggcgtgcg cgagtcgcgg
      181 cgcgacgtcg tgcttcgcgt aggggccggc ggcgtagtcc tcgcgcttca cctggccgcc
      241 gtgcgttgcg tccgcgggcg gcgcgcggtc gtgcagcgcc gcgctgtacg catgacagct
      301 cgcgcaggcg tcgacgttga gcgggaagga caccgcgcgc gtctcccaga cgggcggctc
      361 cttccggccg ccgccgtcgt ggcagctcac gcacgagccg aggaagaggc gcctcccggc
      421 ggcctgctcc ggcgcgagcg tctcggccgg aacggcgagc gcgatctgcc cgcgcgcgaa
      481 cgggtacgcc gcgcgatagc gctcgtggtc gggccagccg ttctcgcgcg tgtggtagcg
      541 cgtgttctcc gacttcttcg cgatcagctc ctcgcgcacg aacgcagcga ccgcggcgat
      601 ctcggcgggc gagaggcgcc ccgcgaagcc gcgcatcgcg gtgcccgggc gcccgtcgcg
      661 cacggcgcgc gcgatgtcct cgcgcgtcag gcgcgacgcg gccggcgcgg tgaagtcgac
      721 cggcttcggc tcgagatagc gcgcggccac ggtgcgcgcg tcgcccgaat agccgtggca
      781 gaaatagcac gagccgttcc acacgctccg gcccagcgcg aggtcgctct gcgccggctg
      841 gcagcccgcg agcagcacgc acagcgccgc ggcgagcgtg cgcatcactt tgccgggcgc
      901 acgaacgccg tgaacacgta ctctgcgacg tcggccatcg tctggtcgtc gatcaccgtg
      961 cgccacgccg gcatctcggt gccgcgccgg ccggcgtaga tcgcggcgta gagcgccggg
     1021 cggttcagct gcgcgcgcga cgacgcgtcg aggaagttgc gcggcttcgg gttgatgaag
     1081 taggcgcgcg gaccctgcgc gtcgcccttc acgccgtggc aggtggcgca gttcgcgtcg
     1141 tagaagcgct tgccgcgggc gcggtcgccc gcgagcccgt tcggcagcgg caggctcatg
     1201 tcggcgcgca tcccctgggg cgcggtcgcc gcggaggggt cgcgcccgcg cccgccgtgc
     1261 gccgacgtgc ccgacacatc gggcgcgggc gcgcccggct gcctcgacat gaacgcgtcg
     1321 cgcacgtagt ccacgacggc ctcgatctcg cgctcgtcga gctgcgtctt ccagccgacc
     1381 atcgccgtgc cgggcttgcc gtcgcgcacg acgcgcacca tccagtcgcg cggcagctgc
     1441 tcgcgcaggc ccggcgcggt gaagtccttc ggcggcggca ccaggctccc gcgcgcgcgg
     1501 ctgcggccgt cgccgcggtc gccgtgacag accgagcagt agttgtggta gatcaggtcg
     1561 ggcttgatcg cgcgcttcgc cgcgggcgcg tcggcggcga gcgacaccgc gggtccgagc
     1621 agcgcgagcg ccgccgaaac ggaggcgacc tgtaggacga ttccgagacg tctcatggcc
     1681 cgaccgagtg tgcccgaccc ccggagccca aatatgacct agatcaaatc gactacggaa
     1741 tcaacttcca gaatgcgggg catgcacaag aacacgcctc gctcagggat cgccttcgcc
     1801 tgcctcgccg ccgccctgct cgcggcctgc gcgcaggtgc cgaccggatc ggagcgcgcc
     1861 gcctcggggc cgcccgtctt cccgtcgccg cccgacgagc cgcgcttcgt ctacgagcga
     1921 agcctctaca gctcgggcga cgtcgtgccc gacgaggcgg gcgcggcgtt ccggcgcatg
     1981 gtgacgggcg agcagcgctc gggcgagggc ctggcgaagc cgtacgcggt cgccgtctat
     2041 cacggccgcg tgtacctgtc cgactcggtc gagcgcttca tcaaggtctt cgacgtcccg
     2101 aacggccgct actacaagat cggcgccgac gacccgggac gcctgtacaa gccgctcggc
     2161 atcgacatcg accgcgccgg caatctctac gtcgccgacg cgacgcagaa ggcgatcctc
     2221 gtctacggcg cgaacggcaa gtacctgcgc aagatcggcg ggccggcggt gttcgaccgg
     2281 ctcgcgagcg tcaccgtcga gcgctcgggc gagcgcgtct acgcggtcga catcggcggc
     2341 gtctcctcgg agctgcaccg cgtgcgcgtc ttcgacgcga agacgggcga gcatctcttc
     2401 gacatcggca agcgcggctc gggcgcgggc gagttcaacc tgccgcgcga cgtcgcgatc
     2461 ggcagggagg ggcgcctcta cgtcgtcgac ggcggcaatt tccgcgtgca ggtgttcgag
     2521 cgcgacggcc ggttcgtgag gagcttcggc tcggtcggcc gccagatggg ccagttcgcg
     2581 cggccgaagg agatcgccgc ggacgcggac ggaaacgtct acgtgatcga caccgcgttc
     2641 ggcaacttcc agatcttcgg ccccgaggga gatctgctca tgtacgtggg gacgcgctcg
     2701 gagcgcgacg gacccgcgcg cttcatgctg ccctcgggcg tgtacgtcga cgaggacggc
     2761 cgcgtctacg tcgtcgacca gtggttccgc aaggtcgacg tcttccgccc ctacgcattg
     2821 tcggcggatt ccggctggct cggccggcgt cccggaacga agtccccggc gaagtaggcg
     2881 cgctttgacc tgcatcaatc cccccatgac cgccgctgca acaatttcgg gcaacgaccg
     2941 ccatgacgaa gaatgcggcc ggcccgcaaa aggaggcgaa catgcaaggg ctgaagcagg
     3001 gatttatccg gggactcgat cgctggggcg gctggatcgc cgcggcgggc gtcgcgctgt
     3061 tcgtggcgac ggcgacgcag tatgcggcgg cgcagacgtc gggcagcatc gcaaacacca
     3121 agcacaatct gtcgagcacc gcggtcgcgg gccagaaccg cgtgacggcg ggcacggacg
     3181 agatctgcgt gttctgccat acgccgcacg gcgcggacac gacggcgccg gcgccgctct
     3241 ggaacaagag cctctcgtcg ctctcgggca acacctacaa gacgtacgac tcgctgggct
     3301 cgacgacgat cgacggcgcg ttcgcgacgg acggcgtcaa caacagcagc gtcggctccg
     3361 tatcgctcgc ctgcctttca tgccacgacg gcacgcaggc gatggacaac atcatcaacg
     3421 cgccgggttc gggcggctac caggtcaacg gcggcggagc gaccggtctc gcctatacct
     3481 gggatacgac caccgggcgc gtggacgcgg acggcaagct gatcgcgaac ttcatcgcga
     3541 acatc
//
LOCUS       DATDMW010000127        13808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1157532, whole genome shotgun sequence.
ACCESSION   DATDMW010000127 DATDMW010000000
VERSION     DATDMW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157532"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VI319_05520"
     CDS             <1..869
                     /locus_tag="VI319_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY6863338.1"
                     /translation="PLLFLYRKEVVDSGGAGRWRGGLSAESCFVPHGTDTIVQDTLSS
                     GNAVPTSPGMMGGYPGSVNVYKFKRGSDVLERLKGRSIPGDIAEVKGENVTLQLRQEN
                     FEQKPADVYAVIWSAAGGFGDPLERDPERVREDVVEHRSVSVQAARDIYGVAIEGERL
                     DVEATRRLRAERRSANRRKDARVPKLAGKVVARLTDNLEARREDDGLHTACAKCAADL
                     GPVRGNYKDACVRREADISAANPNIGDWRRYIDDRPVFRQFFCPGCGALVENEIARAD
                     DEVLRDIEIDLS"
     gene            1022..2272
                     /locus_tag="VI319_05525"
     CDS             1022..2272
                     /locus_tag="VI319_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6863339.1"
                     /translation="MKELIRFTGRLLGVLSIAAALGSAALAQQPIKVGIGISQTGTLG
                     GGGKAALLGLQMWVEDVNKRGGLLGRKVELIAYDDQSNPATTPGIYTKLLDVDKVDLL
                     VAPYGTVPTAPIMPLVKQRGLLLMGNFSFQVNHTVKHDMWFNNAPWGDATSWTEGFLQ
                     VGQKAGAKTIAFIAADQEFAQNLANGAKAVAKKSGLKTVYEQNYPPSTVDFSSMIRAV
                     RNAKPDMVFICSYPNDSVALVRAINEIGVGPSVKLIGGGMVGLQFTPIMENMGSQLNG
                     IVNYTSYAPGMEYPGIDDFFQRYHVKAAEAKVDPLGFYLPPFNYAIGQILEQAVTATK
                     SLDSKTLAKYLRSHEFKTIVGPISWGPDGERAKPSVPMVQFRDLKDKDVNQFLQKGKQ
                     VIVYPPQFKQGDAIVPYDQARKAK"
     gene            2376..3221
                     /locus_tag="VI319_05530"
     CDS             2376..3221
                     /locus_tag="VI319_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6863340.1"
                     /translation="MQGVLLGCFYAAVSIGLSVAFGLLDVPHIAHPAFVVLGSYGTWL
                     VAGRYGWDPLLSGLALTPVFFLIGVAVYRFYYETFEKRGTDTGVRGLAFFFGVAFIIE
                     VGLIIGFGVDQRLVDAPYIGKSLELGEELRVPMRMLVAFGTALALTVLLGLYLSRTFM
                     GRAIKAVAQDEPALRLMGANPVRVKQWAFGIATAVAAVGGALLIIVGPVEPSMDRVYI
                     GRTFCVVVLAGLGSMSGTLVAGLILGISESIVLTLLGASWAPAVSFGLLLLVLGVRPQ
                     GLFGR"
     gene            3240..4193
                     /locus_tag="VI319_05535"
     CDS             3240..4193
                     /locus_tag="VI319_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6863341.1"
                     /translation="MTVRRHYGFWIAAAAALALAATAALTLRNEYYFFAGYVVLQFVI
                     LATAWNILGGYCGYVNFGTGAFFGVGAYTAVLLIKSLEASLPVQIAAAALVGGALGLG
                     TGLLTLRLRGIFFSIATVAVAIILQTVVINWRYLGGATGVQILKPEPLAPFDNYVKML
                     FVIAVALAVAAVAIARYVETSWLGRGLRAIRDNEEAAECTGVPTLKLKLIACSVSGAL
                     MSAAGAPLAMYQSFVEPASAFDLNYSVSALAMPIIGGTAHWIGPVIGAVLLGTVQQLV
                     QVNISSEYNVMVVGVLLVVFVVGAPEGITGLALKLWRRIGR"
     gene            4190..4918
                     /locus_tag="VI319_05540"
     CDS             4190..4918
                     /locus_tag="VI319_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863342.1"
                     /translation="MSAPLLAIDKVSKSFGGFAALTEVSVEIAEGERFGLIGPNGSGK
                     TTLINCVSGALRNDRGSITFAGEEVSRLPAYQRTRRGIARSFQIPRPFRSMTVLENLV
                     VPLEFVVHRHSLHQADTRAEAMEILRGMGLADKADTPSSRLSQVELRKLELARAMAAR
                     PKLLISDEAMAGLSGNEVDEVLRILFKLNETGITIVMIEHIMQAVMRFSQRVICLDAG
                     RIICEGTPESVVKDGGVQRAYLGA"
     gene            4911..5639
                     /locus_tag="VI319_05545"
     CDS             4911..5639
                     /locus_tag="VI319_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863343.1"
                     /translation="MLKLEIRDVEAGYGAVRALHGVSLDIGQGETVALLGTNGNGKST
                     LMKCVMGMVRPTRGAIALELDGERHDLTRLSTERIVDLGIAMVPEGRRLFPRLTVEEN
                     LLLGAFRPAARKDIGKNLAFAFELFPVLAERRRQLAGSMSGGQQQMLAIARALMSSPR
                     LLLVDEPSVGLAPILVSATITKIGELKDRYGLTVLMAEQNFHQAIRIANRGYIIVHGE
                     IVFEGKTVAELEQNPLVKSYYLGA"
     gene            5649..6608
                     /locus_tag="VI319_05550"
     CDS             5649..6608
                     /locus_tag="VI319_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863344.1"
                     /translation="MARQSVRNLPIGMYGAVMGLAGLGLACRAAREVLPVPAFFGEIW
                     VALGVAMLALLLPAYLAKLVRHPGAVREEFLHPSHLGFCGALPVGMALVAGGLAPYWH
                     AGGAALWWISAVMLLAFQVWAMRLFVGGGIPIAEVNGGWLIVMVGGIVVPGPALALGL
                     GGASEFCFGLSAMIAPIIVSLLFYRTVALAPLPPALRPTWFIFLVPPSLIYANGVALS
                     GSTSLFLNGLFYLALALTPALLIASRRFLEWPFAASWWAFTFPLDALATAAAEHARLQ
                     PSALWRGVAGVTLALAVAFVALVLARTVSAFLRGKLFAALKGA"
     gene            6638..6865
                     /locus_tag="VI319_05555"
     CDS             6638..6865
                     /locus_tag="VI319_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863345.1"
                     /translation="MRTEQSVRKLIGQLYQDLGQNPARLKSIKPVGGDWASAASYEVV
                     RDDGAVVHVQGKDIDEANEPQLAEVLGRFRK"
     gene            6978..7184
                     /locus_tag="VI319_05560"
     CDS             6978..7184
                     /locus_tag="VI319_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEY6863346.1"
                     /translation="MARYGKKAQSKVRRAMHERKRGTLKSGRSGKKVKSRKQAIAIGL
                     SEARRAGAKVPRRKSSRGKSRGRR"
     gene            complement(7196..7381)
                     /locus_tag="VI319_05565"
     CDS             complement(7196..7381)
                     /locus_tag="VI319_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863347.1"
                     /translation="MPSCGTVWHTFWTAGVCPGCRWRWEKTQCPACGELAPHKSWYRD
                     PEDRPAERDSEKARLPG"
     gene            7829..9706
                     /locus_tag="VI319_05570"
     CDS             7829..9706
                     /locus_tag="VI319_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6863348.1"
                     /translation="MAERKTRTTKGKIARLARANAVMSAINSAAIRIRDRHALFEEAC
                     NIAVSRGAYRMAWIGLPEGANGALKAAAHAGFNGAARTSALAPLAPDAERGMRRVLRQ
                     GRPHVAQDLAAEERGIPFRKAALARGARSLASLPLRVDGRVAGALLLYAGERDAFDAG
                     ELALAGQLADSIAFALAYIEKEEQLHYLASYDVLTKSANRNLLHDRLAQAIALAGRQG
                     RQVAVIFVDVDNFKYVNDSLGHRAGDLLLKTVTERLGSCVRDVDTIARHGGDEFVVVL
                     PDQSSEEQAGQIIQRILTAIAQPLVIDGQELSITCSLGASFYPQDGADGETLLKNADA
                     AMYRAKELGRNTFQFYGKDINTRVSERLGMETSLRRAIERNELILRYQPQINLTTGRI
                     FGAEALIRWRHPKLGTVPPERFIPLAEETGLIDAIGEWVLRTACAQNKAWQDARLPKI
                     VVSVNLSARQFRQKGFAESISRVLRETGLPSRYLGLELTEGLVMHDTAAGVPILENLK
                     KMGVNLSIDDFGTGYSSLSYLKRFPIDELKIDQSFVRDVMVDPNDATIAKAIIALALE
                     LNLKVTAEGVETEKHIAFLQQHRCDQAQGFYFSRPLPSHEFGALLEQSTLQATSDGRE
                     P"
     gene            9735..10889
                     /locus_tag="VI319_05575"
     CDS             9735..10889
                     /locus_tag="VI319_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY6863349.1"
                     /translation="MPDIDKPKTVPGDPHADNGALVGNDRLIARIRELEAYRHRHETL
                     RKLSSYLKASLAPNDAYAALEAFGHELFPGTTGKLYLAHPPAKYLEVVSAWRDNRSSE
                     QTFTMQDCWALRRAQPHWVREGGGAALLCGHVAPGPKPPAPYLCVPLFAQGETIGMLH
                     LARQKDELAFADASAWLDAALNLAVAAAEEIGLALANLKLRETLHEQSIRDPLTGLYN
                     RRFLEEFLMRELARADRKKHALSIITLDIDHFKRINDSLGHGAGDMVLRRIGLLLQGY
                     VRESDIACRVGGEEFALLLPEVAMPIATQRAEDIRKAVHDMTLKHEDQTLDGITVSLG
                     VAAFPDHASTPEALIRAADQALYDAKYAGRDMVVSAGPESREPRMRQELN"
     gene            complement(10886..11359)
                     /locus_tag="VI319_05580"
     CDS             complement(10886..11359)
                     /locus_tag="VI319_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6863350.1"
                     /translation="MSESDHPVRIRKIGHVGLYCRDLPKMVDFYTRVLGFQVSDVNEK
                     GMTFLRFGADHHSFVLAKMPEEDQKKGAGATVIQQIAMEVASLEALKRIRRHLAAQGV
                     ALKGKIKHEGPGSDYTFDFDDPEGNRLQFFSDMDRIGWDGRSRPKEQWKRFEVED"
     gene            11453..12505
                     /locus_tag="VI319_05585"
     CDS             11453..12505
                     /locus_tag="VI319_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6863351.1"
                     /translation="MRAQRLLLVLLALAGPALAQDAPNRELYLDRGADREQRLIAGAK
                     KEGTLTLYSTMTVSDGKAFADAFERRYGVRLVHWRTSSEKIVQRAVAETRAKRYEADV
                     IESSAPHMEALRREGVLEDFDSPSLAQLVPGAVFPGNRQYVADRLVFFVMGWNTNLVK
                     PSEVPKTYEDLLDPRWAGRITIEGTDVSWFAAVTLAMGEAKGLAYFKRLAAQKPQIRS
                     GHILTAQLVASGEVPFFLTAYNNNIETLKKKGAPIDWRPLQPAFGQAAAVGVAKHAPH
                     PHAALLFADFILSREGQEIYRKVNRVPTSARVDSPLNKFPYQVIDPLLTLDEDGQWSK
                     LFSALFLGGEAVQKGE"
     gene            complement(12545..13492)
                     /locus_tag="VI319_05590"
     CDS             complement(12545..13492)
                     /locus_tag="VI319_05590"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6863352.1"
                     /translation="MTVMNLNVSPVVKGALTFIPGFASLLPERAGETDSARYCYGAWL
                     KHLVMLHQSGARTPLTTLVELGPGRSLGTGLAAMLCGVNHYYALDVVQHASIETNLRI
                     FDELVEMFQKRAPRPAKSWPDYDAHLDARLFPVHILTDEVLKRALTPQRIANIRNAIV
                     QGRSSGPQVTIQYIAPWADESVIAPGTVDLVLSHAVLARVADPAAAYRACYRWLKPGG
                     AMSHQIGFAFPAFTGPWNGYWACPEPLWRIFKGRRPFVMNRLPCSAHLSLMEAQGFRF
                     ACKLKQYRDGVPRSQLAAQWRTLSDDDLRCSGLFVQATK"
     gene            complement(13583..>13808)
                     /locus_tag="VI319_05595"
     CDS             complement(13583..>13808)
                     /locus_tag="VI319_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863353.1"
                     /translation="PETVFKVLKGRRPFVINRLPCSVHLDLMQKQGFRFTCQLKRYEN
                     GLRRSQLAARWRTLSDDDLNCAELFVQATK"
BASE COUNT         2160 a         4745 c         4723 g         2180 t
ORIGIN      
        1 tcccgctgct gttcctgtac cggaaggaag tggtggattc gggcggcgcc gggcgctggc
       61 gcggcggact gtcggccgag tcctgcttcg tgccgcacgg caccgacacc atcgtgcagg
      121 acacgctgtc ctcgggcaac gcggtgccga cctcgcccgg gatgatgggc ggctaccccg
      181 gctccgtaaa cgtatacaag ttcaagcgcg ggagcgacgt gctcgagcgg ctgaagggcc
      241 gctcgatccc cggcgacatc gccgaggtga agggcgagaa cgtcacgctg cagctgcgcc
      301 aggaaaattt cgagcagaag cccgccgacg tctacgcggt gatctggtcg gccgcgggcg
      361 gcttcggcga tccgctcgag cgcgaccccg agcgcgtgcg cgaggacgtg gtcgagcacc
      421 gctcggtgtc ggtccaggcg gcgcgcgaca tctacggcgt cgcgatcgaa ggcgagcggc
      481 tggacgtgga agccacacgg cgcctgcgcg ccgagcgccg ctcggccaac cgccgcaagg
      541 acgcgcgcgt gccgaagctc gcgggcaagg tcgtcgcgcg cctgaccgac aatctcgagg
      601 cgcgccgcga ggacgacggc ctgcacacgg cgtgcgcgaa atgcgcggcc gacctcggac
      661 cggtgcgcgg caactacaag gacgcctgcg tgcgccgcga ggccgacatc tcggccgcca
      721 acccgaacat cggcgactgg aggcgctaca tcgacgaccg cccggtgttc cgccagttct
      781 tctgccccgg gtgcggcgcg ctggtcgaga acgagatcgc ccgtgccgac gacgaggtgc
      841 tgcgcgatat cgagattgac ctgtcctgag tccttcgact cgctcaggac aagctccagc
      901 gagggatccg ttttttgggt tgactggcct ccccgcttga caaataccgc cctgcttcca
      961 taatccggcg gctatctcgc gtttaccaat ccggttcgga cagagctcta aggaggaggt
     1021 catgaaagaa ctcattcgct tcacgggacg cttgctgggc gtcctgtcga tcgccgcggc
     1081 cctcgggtcc gctgcgctcg cgcagcagcc gatcaaggtc ggcatcggca tctcgcagac
     1141 cggcacgctc ggcggcggcg gcaaggccgc gctgctcggc ctgcagatgt gggtcgagga
     1201 cgtcaacaag aggggcggcc tgctcgggcg caaggtcgag ctgatcgcct acgacgacca
     1261 gtcgaacccc gcgaccacgc cgggcatcta caccaagctg ctcgacgtcg acaaggtcga
     1321 cctgctggtc gcgccctacg ggacggtgcc caccgcgccc atcatgccgc tcgtcaagca
     1381 gcgcggcctc ctgttgatgg gcaacttctc cttccaggtg aaccacacgg tcaagcacga
     1441 catgtggttc aacaacgcgc cctggggcga tgcgaccagc tggaccgagg gcttcctgca
     1501 ggtcggccag aaggcggggg cgaagacgat cgccttcatc gccgcggacc aggagttcgc
     1561 tcagaacctc gcgaacggcg ccaaggcggt ggccaagaag agcgggctga agaccgtcta
     1621 cgagcagaac tatccgccga gcacggtcga cttctcctcg atgatccgcg cggtgcgcaa
     1681 cgccaagccc gacatggtgt tcatctgctc gtacccgaac gactcggtcg cgctggtgcg
     1741 cgcgatcaac gagatcggcg tcgggccgtc ggtgaagctg atcggcggcg gcatggtcgg
     1801 cctgcagttc acgccgatca tggagaacat gggcagccag ctgaacggca tcgtcaacta
     1861 cacctcgtat gcgcccggaa tggagtatcc cggcatcgac gacttcttcc agcgctacca
     1921 cgtcaaggcg gccgaggcga aggtcgatcc gctcggcttc tacctgccgc cgttcaacta
     1981 tgcgatcggc cagatcctcg agcaggcggt caccgcgacc aagagcctcg actcgaagac
     2041 gctcgcgaag tacctgcgct cgcacgagtt caagaccatc gtcggcccga tcagctgggg
     2101 acccgacggc gagcgcgcga agccctcggt gccgatggtg cagttccgcg atctgaagga
     2161 caaggacgtc aaccagttcc tgcagaaggg caagcaggtc atcgtgtacc cgccgcagtt
     2221 caagcagggc gacgcgatcg tgccgtacga ccaggcgcgc aaggcgaagt agccggggac
     2281 ccggtcagga cggcgcggcg ctcaccgcgc cgtcttgcat ttcggaccta cgcggagggc
     2341 ttctgttctc gctcgacctg ctcggcgacg cgctggtgca aggcgtcctg ctcggctgct
     2401 tctacgccgc ggtcagcatc ggcctgtcgg tcgccttcgg gctgctcgac gtccctcaca
     2461 ttgcacatcc tgcgttcgtc gtccttggct cgtacggcac ctggctcgtc gccggccgct
     2521 acggctggga cccgctcctg tcgggcctcg cgctcacgcc ggtgttcttc ctgatcggcg
     2581 tcgcggtcta ccgcttctac tacgagacct tcgagaagcg cggcaccgac accggcgtgc
     2641 gcgggctcgc cttcttcttc ggcgtcgcct tcatcatcga agtcgggctg atcatcggct
     2701 tcggggtcga ccagcgcctg gtcgacgcgc cctacatcgg caagagcctc gagctcggcg
     2761 aggagctgcg cgtgccgatg cgcatgctgg tggcgttcgg caccgcgctc gcgctcacgg
     2821 tgctgctcgg gctgtacctg tcgcgcacgt tcatgggccg cgcgatcaag gcggtggcgc
     2881 aggacgagcc cgcgctgcgg ctgatggggg cgaacccggt gcgcgtcaag cagtgggcct
     2941 tcggcatcgc gaccgcggtg gcggctgtgg ggggggcgct gctcatcatt gtcgggccgg
     3001 tcgagccgag catggaccgc gtctacatcg ggcggacgtt ctgcgtggtc gtgctcgccg
     3061 ggctcgggag catgtcgggg acgctcgtcg ccgggctgat cctcggcatc tcggagtcga
     3121 tcgtgctcac gctgctcggc gcgtcgtggg cgccggcggt gtcgttcggg ctgctgctgc
     3181 tggtgctcgg cgtgcggccg caagggctct ttggaagatg agcggaagat gagcggagaa
     3241 tgacggttcg ccggcactac gggttctgga tcgccgccgc cgccgccctc gcgctcgccg
     3301 cgaccgcggc gctcacgctg cgcaacgagt actacttctt cgccggctac gtcgtgctgc
     3361 agttcgtgat cctcgccacg gcgtggaaca tcctcggcgg ctactgcggc tacgtgaact
     3421 tcggcaccgg cgccttcttc ggcgtcggcg cctataccgc cgtgctgctc atcaagtcgc
     3481 tcgaggcgtc gctgccggtg cagatcgccg ccgcggcgct ggtggggggc gcgctcggcc
     3541 tcggcaccgg gctgctcacg ctgcggctgc gcggcatctt cttctcgatc gcgacggtcg
     3601 cggtcgcgat catcctgcag acggtcgtga tcaactggcg ctacctcggc ggcgcgaccg
     3661 gcgtgcagat cctgaagccg gagccgcttg cgcccttcga caactacgtc aagatgctct
     3721 tcgtcatcgc ggtcgcgctc gccgtggcgg cggtcgcgat cgcgcgctac gtcgagacgt
     3781 cgtggctcgg gcgcggcctg cgcgcgatcc gcgacaacga ggaagccgcc gagtgcaccg
     3841 gcgtgccgac gctcaagctg aagctgatcg cctgctccgt gtccggggcg ctgatgagcg
     3901 ccgccggcgc gccgctcgcc atgtaccaga gcttcgtcga gccggcgtcg gccttcgacc
     3961 tcaactattc ggtgtcggcg ctcgcgatgc cgatcatcgg cggcaccgct cactggatcg
     4021 ggccggtgat cggcgcggtc ctgctcggga ccgtgcagca gctggtgcag gtgaacatct
     4081 cctccgagta caacgtgatg gtcgtcggcg tgctgctcgt ggtgttcgtg gtcggcgcgc
     4141 ccgagggcat caccgggctg gctctgaagc tctggcgcag gatcggccga tgagcgcgcc
     4201 gctgctcgcg atcgacaagg tgtcgaagag cttcggcggc ttcgccgcgc tcaccgaggt
     4261 cagcgtcgag atcgccgagg gcgagcgctt cgggctgatt ggccccaacg ggtcgggcaa
     4321 gaccacgctc atcaactgcg tctcgggcgc gctgcgcaac gaccgcggct cgatcacctt
     4381 cgccggcgag gaggtgtcga ggctgcccgc gtaccagcgc acgcgccgcg gcatcgcgcg
     4441 cagcttccag atcccgcggc cgttccgcag catgaccgtg ctcgagaacc tggtcgtgcc
     4501 gctcgagttc gtcgtgcacc ggcattcgct gcaccaggcc gacacccgcg ccgaggcgat
     4561 ggagatcctg cgcggcatgg gcctcgccga caaggccgac acgccctcga gccgcctttc
     4621 gcaggtcgag ctccgcaagc tcgagctcgc gcgcgcgatg gccgcgcgcc ccaagctcct
     4681 catctccgac gaggcgatgg cgggcctctc gggcaacgag gtcgacgagg tgctgcggat
     4741 cctgttcaag ctgaacgaga ccggcatcac catcgtcatg atcgagcaca tcatgcaggc
     4801 ggtgatgcgc ttctcgcagc gcgtgatctg cctcgacgcg ggccgcatca tctgcgaggg
     4861 gacgcccgag tccgtggtca aggacggcgg cgtgcagagg gcgtatctcg gtgcttaagc
     4921 tcgagatccg tgacgtcgag gccggctacg gcgcggtgcg cgcgctgcac ggcgtgtcgc
     4981 tcgacatcgg ccagggcgag accgtggccc tgctcggcac caatggcaac ggcaagagca
     5041 ccctgatgaa gtgcgtgatg ggcatggtgc gcccgacgcg cggcgcgatc gcgctcgagc
     5101 tcgacggcga gcgccacgat ctcacgcgcc tgtcgaccga gcgcatcgtc gacctcggca
     5161 tcgcgatggt gcccgagggc aggcgcctct tcccgcgcct cacggtcgag gaaaacctgc
     5221 tgctcggcgc cttccgcccg gccgcgcgca aggacatcgg caagaacctc gccttcgcgt
     5281 tcgagctctt ccccgtgctc gccgagcgcc gccgccagct cgcgggctcg atgtcgggcg
     5341 ggcagcagca gatgctggcg atcgcgcgcg cgctgatgtc gtcgccgcgg ctgctgctgg
     5401 tcgacgagcc ctcggtcggc ctcgcgccca tcctcgtctc cgccacgatc accaagatcg
     5461 gcgagctgaa ggaccgctac ggcctcacgg tgctgatggc cgagcagaac ttccaccagg
     5521 cgatccgcat cgccaaccgc ggctacatca tcgtccacgg cgagatcgtg ttcgagggaa
     5581 agaccgtggc cgagctcgag cagaacccgc tcgtgaagag ctactacctg ggagcctgag
     5641 ccgcaaccgt ggcgcgccag tccgtccgca acctcccgat cggcatgtac ggggccgtca
     5701 tgggcctcgc gggcctcggc ctcgcgtgcc gcgccgcgcg cgaggtgctc ccggtcccgg
     5761 ccttcttcgg cgaaatctgg gtcgcgctcg gcgtcgcgat gctcgcgctg ctgctcccgg
     5821 cctatctcgc gaagctcgtg cggcacccgg gggcggtgcg cgaggagttc ctgcacccgt
     5881 cgcacctcgg cttctgcggc gcgctgcccg tcgggatggc gctggtcgcg ggcggcctcg
     5941 cgccgtactg gcacgcgggc ggcgcggcgc tgtggtggat ctccgccgtc atgctcctcg
     6001 ccttccaggt ctgggcgatg cgcctcttcg tcggcggcgg catcccgatc gccgaggtga
     6061 acggcggctg gctgatcgtg atggtgggcg ggatcgtggt ccccgggccc gcgctcgcgc
     6121 tcgggctcgg cggcgcctcc gagttctgct tcgggctctc ggcgatgatc gcgccgatca
     6181 tcgtgtcgct gcttttctac cgcaccgtgg cgctcgcgcc gctgccgccg gcgctccggc
     6241 cgacgtggtt catcttcctc gtgccgccgt cgctgatcta cgccaacggc gtcgcgctct
     6301 cgggctcgac cagcctgttc ctgaacgggc tgttctacct ggccctcgcg ctgacgcccg
     6361 cgctgctcat cgcctcgcgc cgcttcctcg agtggccgtt cgccgcgtcg tggtgggcgt
     6421 tcaccttccc gctcgacgcg ctcgcgaccg cggcagccga gcacgcgcgc ctgcagccga
     6481 gcgcgctgtg gcgcggcgtc gcgggcgtga cgctcgcgct cgccgtggcg ttcgtcgcgc
     6541 tggtgctggc gcggaccgtg tcggctttcc tgcgcgggaa gctgttcgcc gcactgaagg
     6601 gcgcttaaga gatacgatcg cgacagagga gggagccatg agaaccgagc aaagcgtgcg
     6661 caagctgatc ggccagctct accaggacct cggccagaac cccgcgcggc tgaagagcat
     6721 caagcccgtc ggcggcgact gggcgtcggc cgcctcgtac gaggtcgtgc gcgacgacgg
     6781 cgccgtcgtg cacgtccagg gcaaggacat cgacgaggcg aacgagccgc agctcgccga
     6841 ggtgctgggg cgcttccgga agtagctccg gacgtcgccc gcgccccgac cggcgcatgc
     6901 gcacgggccc cggctgaaat ccgtctgtcg cgccaacggc ttaggctggg cgcgtcaata
     6961 tcatccggtg acacgacatg gccaggtacg gcaagaaggc gcagagcaag gtgcgccgcg
     7021 cgatgcacga gcgcaagcgc ggcacgctga aaagcggacg ctcgggcaag aaggtgaaga
     7081 gccgcaagca ggcgatcgcg atcggcctct ccgaggcgcg gcgcgcgggc gcgaaggtgc
     7141 cgcgcaggaa atcgtcgcgc gggaagagcc gcgggcgccg ttaagcattc cgcggctagc
     7201 ccggcagccg cgccttctcg gaatcgcgct cggcgggccg gtcctcgggg tcgcggtacc
     7261 agctcttgtg cggtgcaagc tcgccgcacg cggggcattg cgtcttctcc cagcgccagc
     7321 ggcagccagg acagacgccc gcggtccaga aggtgtgcca cacggtgccg cagctcggca
     7381 cgcaggacca gcgatcctcc ggacgcggac gatagccgca tttcgggcag cggattcgga
     7441 ccggacgatg caagtctcgc gccatcttcg aagtttgctt catctgaaag atagacccgc
     7501 ggcgagcgaa tgaaaaggtc cggcgcggct ggtgtgctgt gtccggtttt acattacact
     7561 tcgcttcacg aaggacgcgg ccgaagcaac gatctcggcg gcggccacac ggatcgcggg
     7621 atgcggtaac gtgcgggggg agcttgatgt gtgaacacgt agtcgcgtgc gcattgatgt
     7681 ctgcaagccc tcctgccgcc ggctcccgat cgatcccaga cctcagcttc cccggtttca
     7741 ttccattgtc attgtcgcaa tcccgcgccg cgtcctgcag cggccggcac tgcacgaggt
     7801 gacggatcga tattcccgat cattgagcat ggcggagcga aaaacgagaa caacaaaagg
     7861 gaagatcgcg cgcctggccc gcgccaacgc ggtgatgagc gccatcaatt ccgcagcgat
     7921 acgcatccgc gatcgtcatg cgctgttcga ggaagcgtgc aacatcgcag tctcgcgagg
     7981 ggcctaccgc atggcatgga tcggtctacc ggagggcgcg aacggcgcgt tgaaggcggc
     8041 ggcgcacgcc ggcttcaacg gggcggcgcg cacgagcgcg ctcgcgccgc tcgccccgga
     8101 cgccgagcgc ggcatgcgcc gggtgctgcg ccagggccgg ccccacgtcg cgcaggacct
     8161 cgccgccgag gagcgcggca ttcccttccg caaggccgcc ctcgcccgcg gcgcccgctc
     8221 gctcgcgtcg ctgccgctgc gcgtcgacgg acgcgtcgcc ggggcgctgc tgctctatgc
     8281 cggcgagcgc gacgccttcg acgccggcga gctcgcgctc gccggccagc tcgccgacag
     8341 catcgccttc gcgctcgcgt acatcgagaa agaggagcag ctgcactacc tcgcctcgta
     8401 cgacgtcctc accaagagcg cgaaccgcaa cctgctgcac gaccgcctcg cgcaggcgat
     8461 cgcgctcgcg ggacggcagg gacgccaggt cgccgtcatc ttcgtggacg tcgacaactt
     8521 caagtacgtg aacgactccc tcgggcatcg cgccggcgac ctgctgctca agaccgtgac
     8581 cgagcgcctc ggctcctgcg tgcgcgacgt cgacaccatc gcgcgccacg gcggggacga
     8641 gttcgtcgtg gtcctgccgg accagtcgag cgaggagcag gcgggccaga tcatccagcg
     8701 catcctgacc gcgatcgcgc agccgctcgt gatcgacggc caggagctct cgatcacgtg
     8761 cagcctcggc gcgagcttct acccgcagga cggcgccgac ggcgagacgc tgctcaagaa
     8821 cgccgacgcg gccatgtacc gggcgaagga gctcggccgc aacaccttcc agttctacgg
     8881 caaggacatc aacacgcggg tctccgagcg cctcgggatg gaaaccagcc tgcgccgggc
     8941 gatcgagcgg aacgaattga tccttcgcta ccagccgcag atcaacctga cgacgggccg
     9001 catcttcggc gcagaggcgc tgatccgctg gcgccatccg aagctcggga ccgtaccccc
     9061 cgaacggttc atcccgctcg cggaggagac cggcctgatc gacgcgatcg gcgagtgggt
     9121 gctgcgcacg gcgtgcgcgc agaacaaggc ctggcaggac gcgcggctgc cgaagatcgt
     9181 ggtctcggtg aacctgtcgg cgcgccagtt ccgccagaag ggcttcgccg agtcgatctc
     9241 gcgggtgctg cgcgagaccg gactgccgtc gcgctacctg ggcctggagc tgaccgaggg
     9301 cctggtcatg cacgacaccg cggccggcgt cccgatcctc gagaacctga agaagatggg
     9361 ggtgaacctc tcgatcgacg acttcggcac ggggtactcg agcctctcgt acctgaagcg
     9421 cttcccgatc gacgagctga agatcgacca gtccttcgtg cgcgacgtca tggtcgatcc
     9481 gaacgacgcg accatcgcca aggcgatcat cgcgctcgcc ctggaactca atctcaaagt
     9541 gacggccgaa ggcgtggaga cggaaaagca tatcgctttc ctgcagcagc accggtgcga
     9601 ccaggcccaa ggcttctatt tcagcaggcc cctcccttct cacgagttcg gagcattgct
     9661 ggagcagagc acgctccaag caacgagcga tgggcgagaa ccttagggtc atattttctc
     9721 gccaagcgaa gaagatgccc gacatcgaca agcccaagac cgtgcccggc gatccgcacg
     9781 ccgacaacgg cgcgctcgtg ggcaacgacc ggctgatcgc gcgcatccgc gagctcgagg
     9841 cctaccgtca ccggcacgag acgctgcgca agctctcgtc ctacctcaag gcgagcctcg
     9901 cgccgaacga cgcctacgcg gcgctcgagg ccttcgggca cgagctcttc cccggcacga
     9961 cgggcaagct ctacctggcg catccgccgg cgaagtacct ggaggtggtc tcggcgtggc
    10021 gcgacaaccg ctcctccgag cagaccttca ccatgcagga ctgctgggcg ctgcgccgcg
    10081 cgcagccgca ctgggtgagg gaaggcggcg gcgcggcgct gctctgcggc cacgtcgcgc
    10141 ccggccccaa gccgcccgcg ccctacctgt gcgtgccgct gttcgcgcag ggcgagacga
    10201 tcggcatgct gcacctcgcc cgccagaagg atgagctcgc gttcgccgac gcgtcggcct
    10261 ggctcgacgc ggccctcaac ctcgcggtcg cggcggccga ggagatcggc ctcgcgctcg
    10321 ccaacctgaa gctccgggag acgctgcacg agcagtcgat ccgcgacccg ctgaccggcc
    10381 tgtacaaccg gcgcttcctc gaggaattcc tgatgcgcga gctcgcgcgc gccgaccgca
    10441 agaagcacgc gctgtcgatc atcacgctcg acatcgacca cttcaagcgc atcaacgaca
    10501 gcctggggca cggggcgggc gacatggtcc tgaggcgcat cggcctgctc ctgcagggct
    10561 acgtgcgcga gtccgacatc gcctgccgcg tcggcggcga ggagttcgcg ctgctcctgc
    10621 ccgaggtcgc gatgccgatc gcgacgcagc gcgccgagga catccgcaag gccgtgcacg
    10681 acatgacgct caagcacgag gaccagacgc tcgacggcat caccgtgtcg ctcggcgtcg
    10741 cggccttccc cgaccatgcc tccacccccg aggcgctgat ccgcgccgcc gaccaggcgc
    10801 tctacgacgc gaagtacgcg gggcgcgaca tggtcgtttc ggcgggtccc gagtcgcgcg
    10861 agccgcggat gcggcaggaa ttgaactagt cttccacctc gaagcgcttc cactgctcct
    10921 tcgggcggct cctgccgtcc cagccgatgc ggtccatgtc gctgaagaac tgcagccggt
    10981 tgccctcggg gtcgtcgaaa tcgaaggtgt agtcgctgcc cggtccctcg tgcttgatct
    11041 tgcccttgag cgccacgccc tgggcggcga ggtgccggcg gatgcgcttg agcgcctcga
    11101 ggctggcaac ctccatggcg atctgctgga tcaccgtcgc gccggccccc ttcttctggt
    11161 cctcctcggg catcttcgcc agcacgaagc tgtggtgatc ggcgccgaag cgcaggaacg
    11221 tcatcccctt ctcgttcacg tcggagacct ggaagccgag aacgcgggtg tagaagtcga
    11281 ccatcttcgg cagatcgcgg cagtacaggc cgacgtggcc gatcttgcgg atgcggacgg
    11341 gatggtcgct ctcgctcatg gcgcctcttt ccttcggtgc gttccgcagc gcggaacggg
    11401 ggtcgttcgg gtttgacaaa tgtacaccgc cttaccgcaa aatgttcccg acatgcgcgc
    11461 gcaacgcctg cttctcgttc ttctcgccct cgccggcccg gcgctcgcgc aggacgcgcc
    11521 caaccgcgag ctctacctcg accgcggggc cgaccgcgag cagcggctga tcgccggcgc
    11581 gaagaaggaa ggcacgctca cgctctacag cacgatgacc gtgagcgacg gcaaggcctt
    11641 cgccgacgcc ttcgagcgca ggtacggcgt gcgcctggtg cactggcgca cgtcctcgga
    11701 gaagatcgtg cagcgcgccg tcgccgagac gcgcgcgaag cgctatgagg ccgatgtcat
    11761 cgagagcagt gcgccgcaca tggaggcgct gcgccgcgag ggcgtcctcg aggacttcga
    11821 cagcccgtcg ctcgcgcagc tggtgcccgg ggccgtcttt ccggggaacc gccagtacgt
    11881 cgccgaccgg ctcgtgttct tcgtcatggg ctggaacacg aacctggtga agccgtcgga
    11941 agtgccgaag acgtacgagg acctgctcga cccgcgctgg gcggggcgga tcacgatcga
    12001 gggcaccgac gtgtcgtggt tcgccgcggt gacgctcgcg atgggcgagg cgaagggcct
    12061 cgcctacttc aagcgcctgg cggcgcagaa gccgcagatc cgcagcggcc atatcctcac
    12121 tgcgcagctc gtcgcctcgg gcgaggtgcc gttcttcctc accgcctaca acaacaatat
    12181 cgagacgctg aagaagaagg gcgcgccgat cgactggcgt cctctgcagc ccgcgttcgg
    12241 gcaggcggcc gcggtcggcg tggcgaagca cgcgccgcat ccgcacgcgg cgctgctgtt
    12301 cgccgacttc atcctctcgc gcgagggcca ggagatctac cggaaagtga accgcgtgcc
    12361 gacgagcgcc agggtcgact cgccgctgaa caagtttccc taccaggtca tcgacccgct
    12421 gctcacgctc gacgaggatg ggcagtggtc gaagctgttc tcggcgctgt tcctgggcgg
    12481 cgaggccgtg cagaaaggcg aatagcccgc cggcgagctc gggacgggcg cgtacggcgt
    12541 acggttactt cgtcgcctgg acgaacaggc ccgagcagcg cagatcgtcg tccgacagcg
    12601 tacgccactg cgcggcgagc tgcgagcgtg ggacgccgtc gcggtactgc ttgagcttgc
    12661 acgcgaagcg gaagccctgc gcctccatga gcgacagatg ggccgagcag ggcaggcggt
    12721 tcatcacgaa cggccgccgc cccttgaaga tcctccacag gggctcggga cacgcccagt
    12781 agccgttcca ggggccggtg aaggcgggaa aggcgaatcc gatctggtgg ctcatggcgc
    12841 cgcccggctt gagccaccgg tagcacgccc ggtaggcggc cgcgggatcg gcgacgcgcg
    12901 cgaggacggc gtgcgacagg acgaggtcga ccgtcccggg ggcgatgacg ctctcgtcgg
    12961 cccacggcgc gatgtactgg atcgtgacct gcggtcccga ggacctgccc tggacgatgg
    13021 cattgcggat attggcgatc cgctgcgggg tcagcgcgcg cttgagcacc tcgtcggtga
    13081 ggatgtggac cggaaagagg cgcgcgtcga ggtgcgcgtc gtaatcgggc cagctcttcg
    13141 ccggccgcgg ggcgcgcttc tggaacatct cgaccagctc gtcgaagatg cgcaggttgg
    13201 tctcgatgct cgcgtgctgg acgacgtcga gcgcgtagta gtggttcacc ccgcacagca
    13261 tcgcggcgag acccgtgccg agggagcgcc cgggcccgag ctcgacgagc gtcgtgagcg
    13321 gggtccgcgc tccgctctgg tgaagcatca ccaggtgctt gagccaggca ccgtagcagt
    13381 acctcgcgga gtcggtttcc ccggcgcgct cgggaagcag cgaggcaaat cccgggatga
    13441 aagtcagcgc gcctttcacg acggggctca cgttgaggtt catcactgtc atggccgctc
    13501 cttcctttgg cagcagcctg aaggaagcac gtgccatccg gaacctgcaa acgcccgcga
    13561 cgcttggtcc cttcggctca agctacttgg tcgcctggac gaacagctcc gcgcagttga
    13621 ggtcgtcgtc ggagagggtg cgccagcgcg cggccagctg cgagcgccgc agcccgttct
    13681 cgtaccgctt gagctggcac gtgaagcgaa agccctgctt ctgcatgaga tcgaggtgca
    13741 ccgagcaggg tagccggttg atcacgaacg ggcggcggcc cttgaggacc ttgaacaccg
    13801 tctccggg
//
LOCUS       DATDMW010000128         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1283069, whole genome shotgun sequence.
ACCESSION   DATDMW010000128 DATDMW010000000
VERSION     DATDMW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283069"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="VI319_05600"
     CDS             complement(<1..1332)
                     /locus_tag="VI319_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEY6863354.1"
                     /translation="MPEPIKEISLSRYALQRLHAHPEWAAELADPAPFAREEMSAALE
                     AARGADEAGFKRALRELRQRVLLRAMARDLSGRAALDEVCAAMSDLAEIEIDAALAWL
                     GEPDLVVVGMGKLGGRELNVSSDVDLVFLFRGSMDEQERYERAGRRLIRLLSEPTEDG
                     FAFRVDMRLRPYGESGPLACSFDALELYFVAQGREWERDAWIKARALSGAPEAQRELE
                     KIVRPFVFRKYLDYATLGAMRNLHAEVRREVARRELAEHVKLGPGGIREIEFIAQALQ
                     LVRGGRDPELMARPTLQVLKLLSGKKLLPARAADELSEAYVFLRKVEHRLQYLDDAQR
                     HDLPRDVEDQGRIARMSGFASWDEFARRLAEARTRVSSHFEDVLADPASDQAYVPWPD
                     HPRLAAFRASQRYATLPAESKRRVDALVPALAKACGSEATLARALFGEAVRE"
     gene            1370..>2098
                     /locus_tag="VI319_05605"
     CDS             1370..>2098
                     /locus_tag="VI319_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY6863355.1"
                     /translation="MKRLLQALEVLAWLMFFAFAGLVLALRFWLLPDIEHYRGDIVAA
                     VTRAVGQPVKIGAIEAGWLGLRPQVSLYDVRVYDSAGQEALVLPVVDNVVSWRSIARG
                     DLRLHSLAIDSPRLSVRRDAAGELYVAGIKLAASARGDSRLADWVLDQAEISVRNAQI
                     EWLDEKRNAPPLTLTEVDVRLRNRGNEHLVGVSARPPARLGAALDVRAELAGRSLAEF
                     SAWNGKLYLALGATDLSAWRAWVDY"
BASE COUNT          289 a          739 c          750 g          320 t
ORIGIN      
        1 ttcgcgcacc gcctcgccga agagcgcgcg cgcgagcgtg gcctccgagc cgcaggcttt
       61 cgccagcgcc ggaacgagcg cgtcgacccg gcgcttcgat tcggcgggca gcgtggcata
      121 gcgctggctc gcgcggaacg ccgcaaggcg cggatggtcc ggccacggga cgtacgcctg
      181 atccgacgcg ggatccgcga gcacgtcctc gaagtggctc gagacgcgcg tgcgcgcctc
      241 cgcgaggcgg cgcgcgaact cgtcccacga cgcgaagccg ctcatccgcg cgatgcggcc
      301 ctggtcttcc acgtcgcgcg gcaggtcgtg gcgctgcgcg tcgtcgaggt attgcaggcg
      361 gtgctcgacc ttgcgcagga acacataggc ctcggagagc tcgtccgccg cccgcgcggg
      421 aagcagcttt tttccggata gcaatttcag aacctggagc gtgggcctcg ccatgagctc
      481 ggggtcgcgc ccgccgcgca cgagctgcag cgcctgcgcg atgaactcga tctcgcggat
      541 gccgcccggc ccgagcttga cgtgctcggc gagctcgcgg cgcgccactt cgcggcgtac
      601 ctccgcgtgc aggttgcgca tcgcgccgag cgtcgcgtag tcgaggtact tgcggaacac
      661 gaacggccgg acgatcttct cgagctcgcg ctgcgcctcg ggcgcgccgc tcagcgcgcg
      721 cgccttgatc caggcgtcgc gctcccactc gcggccctgc gcgacgaaat agagctcgag
      781 cgcgtcgaag ctgcacgcga gcggcccgga ttcgccgtag gggcgcagcc gcatgtcgac
      841 gcggaacgcg aagccgtctt cggtcggctc cgagagcagg cggatcagcc tgcgcccggc
      901 gcgctcgtag cgctcctgct cgtccatcga gccgcggaag aggaacacga ggtcgacgtc
      961 ggaggagacg ttgagctcgc ggccgccgag cttgcccatg ccgaccacca cgaggtccgg
     1021 ctcgccgagc catgcgagcg ccgcgtcgat ctcgatctcg gcgaggtcgc tcatcgcagc
     1081 gcacacctcg tcgagcgcgg cgcgcccgga caggtcgcgc gccatcgcgc gcagcaggac
     1141 gcgctggcgc agctcgcgca gcgcgcgctt gaagccggct tcgtcggcgc cgcgcgccgc
     1201 ctcgagcgcg gcgctcattt cctctcgcgc gaacggcgcc gggtcggcga gctcggcggc
     1261 ccattccgga tgcgcgtgca ggcgctgcag cgcatagcgt gaaaggctga tttctttaat
     1321 cggttccggc atttatcgct aaaatgggcg cctaagagtg aattccacca tgaagcggct
     1381 cctgcaggcg ctcgaagtcc tcgcctggct gatgtttttc gcgtttgcgg ggctcgtcct
     1441 cgcgctgcgt ttctggctgc ttcccgatat cgagcactat cgcggcgaca tcgtcgccgc
     1501 ggtcacgcgc gccgtcgggc agccggtgaa gatcggcgcg atcgaggcgg gctggctcgg
     1561 cttgcggccg caggtcagcc tgtacgacgt ccgagtctac gacagcgcgg ggcaggaggc
     1621 gctcgtgctt ccggtcgtgg acaacgtagt ctcgtggcgc tcgatcgcgc gcggcgacct
     1681 gcgcctgcat tcgctcgcca tcgacagccc gcgcctgagc gtgcgccgcg acgccgcggg
     1741 cgagctctac gtggcgggca tcaagctcgc ggcgtccgcg cgcggcgatt cgcgcctcgc
     1801 cgactgggtg ctcgaccagg ccgagatcag cgtgcgcaac gcgcagatcg aatggctcga
     1861 cgagaagcgc aacgcgccgc cgctcacgct gaccgaggtg gacgtgcggc tgcgcaaccg
     1921 cggcaacgag cacctggtgg gtgtttcggc gcggccgccg gcccgtctcg gcgcggcact
     1981 ggacgtgcgc gccgagctcg ccgggcgcag cctggccgaa ttctcggcgt ggaacggcaa
     2041 gctgtatctc gcgctcggcg cgacggacct ctcggcatgg cgcgcgtggg tcgactat
//
LOCUS       DATDMW010000129         3747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2155493, whole genome shotgun sequence.
ACCESSION   DATDMW010000129 DATDMW010000000
VERSION     DATDMW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2155493"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..497)
                     /locus_tag="VI319_05610"
     CDS             complement(54..497)
                     /locus_tag="VI319_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6863356.1"
                     /translation="MFKHILIPTDGSPTSAKAVEGGIELARQLGARVTGFHACEPALA
                     RVYGDGYMIGPRDAAGSAEHANEAGERAVAQIAAAAQAAGVPFERLMVEADSAYRGII
                     DAATSRGCDAIFMASHGRSNIAELVLGSVTHQVLSHSKIPVLVYR"
     gene            complement(561..1175)
                     /locus_tag="VI319_05615"
     CDS             complement(561..1175)
                     /locus_tag="VI319_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY6863357.1"
                     /translation="MNDPVAYWHAEHGYYTRLLDILDREVAAFHAGRKPDYTLMRDIV
                     RYLRYFPDRFHHPREDEAFRRLAARDPGMALPLSRLLQEHRVIGEAGDQLLWRLEQVA
                     EDVVVSRAIVESMAATYLVYYRHHIATEEREVLPRAAALLAAADWSAIAAAAPEGIDP
                     LFGGEDCAPRYRDLRRRIAQEADAAAREAAASEPLPGPVFDTNQ"
     gene            1300..2067
                     /locus_tag="VI319_05620"
     CDS             1300..2067
                     /locus_tag="VI319_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6863358.1"
                     /translation="MRDIPIYRSALFAAAVAAAWAALAGAALAQDGERSGKQIVEEQC
                     VKCHRTGVNGAPKIGDRDAWIPRMKQGLDALVRAAIRGHGGMPARGGRADLTDSEFRA
                     AILYMFNPTAAAAAPAESGAPPAQPPGPNHKTVGSLDVDLGIVPARSLLAFPRDSVER
                     SMHGGVPSGKDYYHLNVSLADARTHAPVGDASVAVDIEEPGITRTSTRLEPMAVGPAS
                     YGNYVRLKPHTAYLITVHVRAPSAPPGAQAQFEHRTD"
     gene            2092..2451
                     /locus_tag="VI319_05625"
     CDS             2092..2451
                     /locus_tag="VI319_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY6863359.1"
                     /translation="MEKPLQIVMRHLEPSLALEARIREHIARLETVHPHIVGCRVAVE
                     ALRRHDRQGHAFQVRIDARVPGKEYVATRKHDSDVMLALHEAYHALRRQLEDDIRLKR
                     GEVKQHAPRRAPEENGA"
     gene            2448..2942
                     /locus_tag="VI319_05630"
     CDS             2448..2942
                     /locus_tag="VI319_05630"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEY6863360.1"
                     /translation="MKNGRAGFRPQRREQLLDDPRHDAYRASGKLPDPTRCPHCGASF
                     QNGRWTWNAAPRDAHEHACPACRRTRDGFPAGYVSLAGEYFAAHRGEILNLVRNCEAA
                     EKIEHPMQRIIAIDDDDGGVLVKTTDAHLARNIAERVHDACKGALAVQYSRQENLFRA
                     NWRR"
     gene            complement(2943..>3747)
                     /locus_tag="VI319_05635"
     CDS             complement(2943..>3747)
                     /locus_tag="VI319_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY6863361.1"
                     /translation="QGRLAREGFVSATAREQAELALRTAERTLEAAKFAEDAAGHEVE
                     QARAALVRYRAESGARVPSATAWEIKSPATGRVLKVVQESEGAVALGAPLLEIADSRS
                     LEAIVDVLSQESVAIRPGMPARIDVGQGVAPLAGLVRLVEPAAFTKISALGVEEQRVN
                     VVLDFAEPLDRVQTIGDGFRVEAHIVTHRVDDAVRVPVGALFRDGDGWAVFAVDGTRA
                     VKRPVKAPRRNGLEAQIEAGLSAGDKVVVYPPDTLKDGARIREQEKKAR"
BASE COUNT          576 a         1370 c         1317 g          484 t
ORIGIN      
        1 aatctcgccg aggcgatcgg gcgccgcctg cggcgccggc gttcgcacgg cggtcaacgg
       61 tagacgagca ccgggatctt cgagtgcgag agcacctggt gcgtgacgct gccgagcacc
      121 agctcggcga tgttcgagcg cccgtgcgag gccatgaaga tcgcgtcgca gccgcggctg
      181 gtcgcggcgt cgatgatgcc gcggtacgcc gagtcggcct cgaccatcag gcgctcgaac
      241 ggaaccccgg cggcctgtgc cgccgccgcg atctgcgcaa ccgccctttc gcccgcctcg
      301 ttggcgtgct ccgccgagcc cgccgcgtcg cgcgggccga tcatgtagcc gtcgccgtag
      361 acgcgcgcga gcgcaggctc gcaagcgtgg aagccggtga cgcgggcgcc gagctgccgg
      421 gcgagctcga tgccgccctc caccgccttg gccgaggtcg gcgagccgtc agtcggtatc
      481 agtatgtgct tgaacatcgt tcctccgtcg cggcaacgcc gatcacgatg caggagatcg
      541 tacgcgcggc aggaccgccg ctattgattt gtatcaaaaa ccgggcccgg cagcggctcg
      601 ctcgccgcgg cttcgcgggc cgccgcgtcc gcctcctgcg cgatgcgccg gcgcaggtcg
      661 cggtagcgcg gcgcgcagtc ctcgccgccg aacagcgggt cgatgccttc gggcgcggcc
      721 gcggcgatcg ccgaccagtc ggccgcggcg agcagcgcgg ccgcgcgcgg cagcacttcg
      781 cgctcctcgg tcgcgatgtg gtggcggtag tagacgaggt aggtcgccgc catggactcg
      841 acgatcgcgc gcgagacgac gacgtcctcg gcgacttgct cgagccgcca cagcagctgg
      901 tcgcccgcct cgccgatgac gcggtgctcc tgcagcaggc ggctcagcgg cagcgccatg
      961 ccgggatcgc gcgccgcgag gcggcggaac gcctcgtcct cgcgcggatg gtggaagcgg
     1021 tcggggaagt agcgcaggta gcgcacgatg tcgcgcatca gcgtgtagtc gggcttcctc
     1081 ccggcgtgga aggcggcgac ctcgcggtcg aggatgtcga gcagccgggt gtaatacccg
     1141 tgctcggcgt gccagtaggc gacaggatcg ttcatggcga aagcggaggg gttgccgtgt
     1201 cgattcaggc tagtcgcgcc agcgcgcgtg ctcttgattc acgtcaaccc gacgcgctcg
     1261 aaacggcgga ggctaaggga cacgggaaag gaagacacca tgagagacat cccgatttac
     1321 cgaagcgcgc tcttcgccgc agcggtcgcg gccgcatggg cggccctcgc cggcgccgcg
     1381 ctcgcgcagg acggtgagcg cagcggcaag cagatcgtcg aggagcagtg cgtgaaatgc
     1441 caccggaccg gcgtcaacgg cgcgccgaag atcggcgacc gcgacgcgtg gatcccgaga
     1501 atgaagcagg ggctcgacgc gctcgtgcgc gccgcgatcc gcgggcacgg cggcatgccg
     1561 gcgcgcggcg gacgcgccga tctcacggac tccgagttcc gtgcggcgat cctctacatg
     1621 ttcaacccca cggccgcggc cgccgcgccc gccgagagcg gagcgccgcc cgcgcagccg
     1681 ccgggaccga accacaagac cgtcggcagc ctggatgtcg acctcggcat cgtgccggcg
     1741 cgctcgctcc ttgcgttccc gcgcgactcg gtcgagcgct cgatgcacgg cggcgtgccg
     1801 agcggcaagg actactacca cctcaacgtc tcgctggccg atgcgaggac ccacgctccc
     1861 gtcggcgacg ccagcgtcgc ggtcgatatc gaggagcccg gcatcacgcg cacgtcgacc
     1921 cggctcgagc ccatggcggt cgggccggcg agctacggca actacgtgcg cctgaagccg
     1981 cacacggcct acctcatcac ggtgcacgtg cgcgcgcctt ccgcgccgcc gggcgcgcag
     2041 gcgcagttcg agcaccggac agactgagaa aaacagcttc ggaaagagac cgtggagaaa
     2101 ccgctgcaga tcgtgatgcg ccacctcgag ccctcgctcg cgctcgaagc gcgcatccgc
     2161 gagcacatcg cgaggctcga gaccgtccat ccgcatatcg tcggctgccg cgtcgcggtc
     2221 gaggcgctgc gccggcacga ccggcagggc cacgcattcc aggtacgcat cgacgcgcgc
     2281 gtgcccggca aggagtacgt ggcgacgcgc aagcacgaca gcgacgtcat gctggcgctg
     2341 cacgaggcct accacgccct gcgccgccag ctcgaggacg acatccgcct gaagcgcggc
     2401 gaggtcaagc agcacgcgcc ccgccgcgcg cccgaggaga acggcgcatg aagaacggac
     2461 gcgcaggatt ccggccgcag cgccgcgagc agctgctcga cgatccgaga cacgacgcct
     2521 accgcgcgag cgggaagctc cccgacccga cgcgctgccc gcactgcggc gcgagcttcc
     2581 agaacggccg ctggacgtgg aacgccgcgc cgcgggacgc gcacgagcac gcctgccccg
     2641 cgtgccggcg cacgcgagac ggctttcccg cgggctacgt cagcctcgcc ggcgagtact
     2701 tcgccgcgca ccgcggcgag atcctcaacc tcgtcaggaa ctgcgaggcg gcggagaaga
     2761 tcgagcaccc gatgcagcgc atcatcgcga tcgacgacga cgacggcggc gtgctggtga
     2821 agaccaccga cgcgcatctc gcgcgcaaca tcgccgagcg cgtgcacgac gcgtgcaagg
     2881 gcgccctcgc ggtccagtac agccggcagg agaacctgtt ccgcgcgaac tggcggcgct
     2941 gatcagcgcg ccttcttctc ctgctcacgg atgcgcgcgc cgtccttgag cgtgtcgggc
     3001 ggatagacga ccactttgtc gcccgcgctc agacccgctt cgatctgcgc ctcgaggccg
     3061 ttgcgccgcg gcgccttcac cgggcgcttc accgcccgcg ttccgtcgac ggcgaacacg
     3121 gcccagccgt cgccgtctcg gaagagcgcg ccgacgggca ccctgacggc gtcgtcgacg
     3181 cgatgcgtga cgatgtgcgc ctcgacccgg aagccgtcgc ctatcgtctg tacgcgatcg
     3241 agcggctcgg cgaaatcgag cacgacgttg acgcgctgct cctccacgcc gagcgcggag
     3301 atcttcgtga acgccgccgg ctccaccagg cgcaccaggc cggcgagcgg cgccacgccc
     3361 tgaccgacgt cgatcctcgc cggcatgccc ggccggatgg caaccgactc ctgggacagg
     3421 acatccacga tcgcctcgag gctgcgggag tcggcgatct cgagcagcgg cgcgcccagc
     3481 gcgacggcgc cctcgctttc ctggacgact ttcaggacac ggccggtcgc gggcgacttg
     3541 atttcccacg ccgtggcgct cggcacgcgc gccccggact ccgcgcggta gcgcacgagc
     3601 gcggcgcgcg cctgctcgac ctcgtgcccc gcggcgtcct cggcgaactt cgccgcctcg
     3661 agcgtgcgct cggcggtgcg cagcgcgagc tcggcctgct cgcgcgccgt cgccgagacg
     3721 aagccttcgc gcgcgagcct gccctgg
//
LOCUS       DATDMW010000130        10678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1408078, whole genome shotgun sequence.
ACCESSION   DATDMW010000130 DATDMW010000000
VERSION     DATDMW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408078"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1723
                     /locus_tag="VI319_05640"
     CDS             245..1723
                     /locus_tag="VI319_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY6863362.1"
                     /translation="MALLAALAPAAARAEAASSAAALPDFLWSSAPLEAKTKPILDMP
                     SSPRDASLQRREVRDPITLPRALDLTQSPDDLWDRIRRGFSMPNLDSPLVRNRQVYYA
                     SQKAYFERMVARSRRYMYHIVEEIEKRGMPTELALLPMVESAFNPMAYSRAHASGLWQ
                     FIPSTGKNYKLKQNWWFDERRDILASTTAALDYLQSLYDTYGDWHLALASYNWGENAV
                     ARAIAKNRKKGLPTDYLHLSMPRETKYYVPKLQALKNIVMNPAAYGLSLDPIPNEPYF
                     VTVNTDKDIDIRVAAQLAEMPIEELIALNPAYKRPVMAGSQRLTLVLPADRVEVFEEN
                     LENHSDPLVSWQTYTLKHGDKLEKLAARHGITLARLKLVNGIGKRTRVTPGMQIMLPI
                     KGADIGNLEPLPAAFFQPTPDAPRRGGKKLVHVVKPGETLFGIARRYRVTVDDLKRWN
                     HALGAALSAGQRIFIYRGPAKRMSSTHVKPVAKLKAKATSPA"
     gene            complement(1731..3599)
                     /locus_tag="VI319_05645"
     CDS             complement(1731..3599)
                     /locus_tag="VI319_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863363.1"
                     /translation="MSELLLRVQGLKTVLYGAGEPVRAVDGIDLEIRRGETFALVGES
                     GSGKSMTALSIMRLLPETGRIAAGTVELEGTDLLALPEAAMRVVRGRRVAMIFQEPGT
                     SLNPVLTVGYQIREVLQRHTPLKGAAIDARVVELIESVGIPDAGRRATAYPFQLSGGM
                     KQRVMIAMALAAGPDLLIADEPTTALDVTIQAQILELLRDLQKKTGMSMLLITHDLGI
                     VSQMAQRVAVMYAGHLVEVADRERFFSAPQHPYSRKLFAALPDAGKRGGELSVIKGQV
                     PPLTADFRQCRFAERCDFAFERCFREDPGWTRTGPGPDHPQIVRCHLREEKGPAGAMD
                     EAQAIAAAAREAATAKPLLSVRDLKVHFPIRKGVLRRTVGWVKAVDGVSLEIGAGRTH
                     ALVGESGSGKTTAGKAILQLIPATAGEVVFDGVELTKLARGELRARRGDFQIIFQDPY
                     SSLNPRMRIADILSEGMTALGVESGAAARSKRVDELLEQVGLPRDSRERYPHEFSGGQ
                     RQRIAIARALAVRPRLIVCDEPTSALDVSVQAQILNLLKSLQRDLGLSYLFITHNIAV
                     VEYLAHEVSVMYLGRIVEHGPARQVLESPAHPYTQALLAAVPSVADDVEKRGKRDA"
     gene            complement(3596..4993)
                     /locus_tag="VI319_05650"
     CDS             complement(3596..4993)
                     /locus_tag="VI319_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6863364.1"
                     /translation="MPVILWTDALVFVLVAALALYSWYARRQPHLRRPWGRVTQSAAG
                     MAALVVLVVYVAIGLLDSLHYRPALDRKGERGETAYSTEVRSVFDALATPLWAHRERT
                     YSAPLATRAFARETLDVPGPGGKTLQVREYPRLAWGGAHLKEESDRGRDIALRAAGGL
                     AVAVLVWAGLGTLVSRRHSWRAVWSGGTGFPWAAVLLTAGALLALAGPMLALCGYYHV
                     LGTDKVGRDVLYLAMKSVRTALVIGTVTTLMLLPFAIALGIMAGYFRGWIDDVIQFVY
                     TVINSIPGVLLIAAFILLAQVFLDQHAGLFPTSVQRADVRLLVLCGVLGITGWTTLAR
                     LLRGETLKLRELEFVQAAQAFGVRDTRIMTRHVLPNVMHIVLISLAIDFSGLVLAEAV
                     LSYVGVGVDPSMISFGTMINAARLEMSREPMVWWTLGAAFVFMFALVLAANLFADAVR
                     DAFDPRTRVGGLLPV"
     gene            complement(5004..5981)
                     /locus_tag="VI319_05655"
     CDS             complement(5004..5981)
                     /locus_tag="VI319_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6863365.1"
                     /translation="MIAYIVRRALYAIPILIGVNLLTFFLFFKLNTPDDMARLQLGAR
                     RITAEAVERWKAEHGYDKPTWYNDRATGLARVTSTIFFEKSVRLFVFDFGNADDGRSI
                     RHEISTRMWPSLAVQLPVFLVGLAVNITFALLMAFFRGTYLDFWGVVLCVAMMSISSL
                     FYIIGGQYVFSKIWNLVPISGYSSGPDAFKFLVLPVAIGAVSGIGSGARWYRTIFLEE
                     IGKDYVRTARAKGLAETVVMFRHVLKNGLIPILTGAVVVIPTLFLGALITESFFGIPG
                     LGSYTIDAIQNQDFLVVRSMVFLGAVLYIVGLLLTDISYTVADPRVRFE"
     gene            complement(5978..8215)
                     /locus_tag="VI319_05660"
     CDS             complement(5978..8215)
                     /locus_tag="VI319_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6863366.1"
                     /translation="MRGPFLRQLVLLALLACGLTGCGRVWNDPYPADERGRDILYTFF
                     IERPKHLDPAQSYTSDEYDIIQEIYEPPLQYHYLKRPYELEATTATEVPKPRYLDANG
                     RPLPADSDRVAYSEYDIHIKPGIMYQPHPAFATDDRGEPLYLSLAEGDVRSKYKLSDF
                     PKTGSRELVAEDYVYQIKRLAHPRIVSPIYGHMTDYIVGLKDLGDRLRRDNAVIAAAH
                     RERYGAADTQLPWLDLRGYELSGVHAVDRYTYRVRIKGKYPQFVYWLAMPFFAPMPWE
                     ADRFYSQRGMSEGRNLTLDWYPVGTGPYMLAENDPNSRMVLAANPNFHGEAYPSEGEP
                     GDAGAGLLADAGKRLPLNERIVFTREKESIPLWNKFLQGYYDAAGIGSDNFDQAVRIS
                     VGGDATLSPEMEERGIRLRTSVATSTFYLAFNFLDPVVGNKGDARARERARKLRQAVS
                     IAIDWEEYISIFANGRGLPGMGPLPPGIFGYHEGEAEYNPVVYERGPDGKPRRKGVEA
                     AKKLLAEAGYPDGRDAKTGEPLVLYLDTTERGPDDASRLNWYRRQFAKIDVQLEIRST
                     DYNRFQDKIRSGNTQMFFLGWNADYPDPENFMFLLYGPNGTQDGENKARYSNPVFDRL
                     FEQMKNMENGPKRQAIIDEMVKLVRDDAPWVWGFHPKDYVLSHAWVHNSKPNKMARNG
                     LKYIRVDAALREAKRREWNRPDVWPLALILLALVLGSVPAWMSYRRRERIVARPAAQV
                     RQTAA"
     gene            8307..9104
                     /gene="fabI"
                     /locus_tag="VI319_05665"
     CDS             8307..9104
                     /gene="fabI"
                     /locus_tag="VI319_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY6863367.1"
                     /translation="MALLEGKRILVTGLLSNRSIAWGIAKAAKREGAQLAFTYQNERF
                     KDRVTEMAAELGSRLAVPCDVASDDEIARLFESLRREWDGLDGVVHAIAFAPREAIAG
                     DFLDGFSREAFRVAHDVSAYSFGALAKGALPLMQGRHGALLTLTYLGAERTVPNYNTM
                     GLAKASLEASVRYLAASLGPRGMRVNAISAGPIKTLAAAGIAGFGKILKHVEEHAPLR
                     RNVTIDDVGNAAAFLLSDLAAGVTGEVLHVDAGFSTTVGGMPTEAGA"
     gene            complement(9118..>10678)
                     /locus_tag="VI319_05670"
     CDS             complement(9118..>10678)
                     /locus_tag="VI319_05670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6863368.1"
                     /translation="QLRDAITSIPAFQEDGKFSSARYETLLRGEGYSPASFEASVRRD
                     LMLQQLTGALSDSGIASKAVAQRLARLSQQQREVAESVIAADPKAREAQPSAEAVRAY
                     YDQNPAQFQVPEQISVEYVVLDLDALAAAETIDPAAVKSYYEQNAAKYGEAEQREASH
                     ILLSYKPNATDADKAKVRAKAEQVLAEVRKSPGAFAALAKKYSEDPGSAPKGGDLGWF
                     ARGMMVPQFEDAVFRMKPNQISGLVESEFGLHIIKLTGVKPGKKRPLEEVRPEIEREL
                     KRQAAQRRYPEAAESFSNTVYEQPDSLKPAADKFHLQIRRADGVTRQSAPVQVLNAPR
                     VLQALFSEDVITNKRNSEAVEAAPNALVAARMVEHKAASQRPFEAVKSDIQRLLAQRQ
                     AREIAAKKGAERLAELKKGNAAGVAFAPAKTVSREKTAGLAPAAVSRIFSADASALPA
                     YVGVEVPAGYALYRVGKVIDVEPDEARQRAVQSELGRANGEEEFRSFLTGLRSDVGVE
                     INREAIEKKPQ"
BASE COUNT         1698 a         3725 c         3635 g         1620 t
ORIGIN      
        1 cctcgagaga ggcgtctccc gggcccgacg aaacgccgat gtccatacac cgataggaac
       61 caaagctgcc accgggcggt cgattcgata cgcgcacgac attcaggttt tccccgagcc
      121 gggctaaggt taaaaacccc tttttattga gtaacttcct tgaccaggct cgagaggctg
      181 gataccatgc gcgctgccac cgaggactga cggagccctc ttgggacgct gcataccgat
      241 tgtgatggcc ctgctcgccg cgctggcgcc tgccgccgcg cgcgccgaag ccgcgtcgtc
      301 cgcagccgct ctccccgatt tcctgtggtc gagcgctccg ctcgaggcca agacgaagcc
      361 gatcctcgac atgccgtcgt cgccgcgcga cgcgtcgctg cagcggcgcg aggtgcgcga
      421 tccgatcacg ctgccgcgtg cgctcgacct cacccagtcg cccgacgacc tgtgggatcg
      481 catccgccgc ggcttctcca tgccgaatct cgacagcccg ctggtgcgca accgccaggt
      541 ctattacgcg agccagaagg cgtacttcga gcgcatggtg gcgcgcagcc ggcgctacat
      601 gtatcacatc gtcgaggaga tcgagaagcg cggcatgccg accgagctcg cgctgctgcc
      661 gatggtcgag agcgcgttca acccgatggc ctactctcgc gcgcacgcct cgggcctgtg
      721 gcagttcatc ccgtcgacgg gcaagaacta caagctgaag cagaactggt ggttcgacga
      781 gcggcgcgac atcctcgcct ccaccaccgc cgcgctcgac tacctgcagt ccctgtacga
      841 cacctatggc gactggcacc tcgccctcgc ctcctacaac tggggcgaga acgcggtcgc
      901 gcgggcgatc gcgaagaaca ggaagaaagg cctgccgacc gactacctgc acctgtcgat
      961 gccgcgcgag accaagtact acgtgcccaa gctgcaggcg ctcaagaata tcgtgatgaa
     1021 cccggccgcc tacggcctca gcctcgaccc gatcccgaac gagccctact tcgtcaccgt
     1081 caacaccgac aaggacatcg acatccgcgt cgcggcccag ctcgccgaga tgccgatcga
     1141 ggagctgatc gcgctcaacc ccgcgtacaa gcggccggtg atggcgggct cgcagcgcct
     1201 gacgctggtt ctccccgcgg accgcgtcga agtcttcgag gagaacctcg agaaccattc
     1261 cgacccgctc gtctcctggc agacctacac cctgaagcac ggcgacaagc tcgagaagct
     1321 cgccgcgcgg cacggcatca cgctggcgcg cctgaagctc gtgaacggca tcggcaagcg
     1381 cacgcgcgtc acgccgggaa tgcagatcat gctgccgatc aagggcgccg acatcggcaa
     1441 cctcgagccg ctgcccgcgg cgttcttcca gccgacgccc gacgcgccgc gccgcggcgg
     1501 caagaagctc gtgcacgtcg tgaagcccgg ggagacgctg ttcgggatcg cgaggcgcta
     1561 ccgcgtcacc gtcgacgacc tcaagcgctg gaaccacgcg ctcggcgcgg cgctatccgc
     1621 gggccagcgg atcttcatct accgcggccc ggcgaagagg atgtcgtcca cgcacgtcaa
     1681 gcccgtcgcc aagctcaagg ccaaggccac ctcgcccgcc tgagcgcgtc tcaggcgtcc
     1741 cgcttccccc gcttttctac atcgtccgcg acgctcggca ccgccgcgag cagcgcctgc
     1801 gtataggggt gcgcgggcga ttccagcacc tgccgcgcag gaccgtgctc gacgatgcgg
     1861 ccgaggtaca tcaccgacac ctcgtgcgcc aggtactcga ccaccgcgat gttgtgcgtg
     1921 atgaagaggt acgacaggcc caggtcgcgc tgcagcgact tcagcaggtt gaggatctgc
     1981 gcctgcaccg agacgtcgag cgcgctggtc ggctcgtcgc agacgatcag ccgcgggcgc
     2041 accgccagcg cgcgcgcgat cgcgatgcgc tggcgctggc cgcccgagaa ctcgtgcggg
     2101 tagcgctcgc gcgaatcccg gggaaggccg acctgctcga gcagctcgtc cacgcgcttc
     2161 gatcgcgcgg cggcgccgga ctcgacgccg agcgccgtca tgccctccga gaggatgtcg
     2221 gcaatgcgca tccgcggatt cagcgacgag tacggatcct ggaagatgat ctggaaatcg
     2281 ccacggcgcg cgcgcagctc gccgcgggcg agcttcgtca gctcgacgcc gtcgaacacg
     2341 acctcgccgg cggtcgcggg gatcagctgc aggatcgcct tgcccgcggt ggtcttgccc
     2401 gatcccgact cgccgacgag cgcgtgcgtg cgtccggcgc cgatctcgag ggagacaccg
     2461 tcgaccgcct tgacccagcc gacggtgcgg cgcaggaccc ctttgcggat cgggaagtgc
     2521 accttgagat cgcgcaccga gagcagcggc ttcgcggtcg ccgcctcgcg cgccgcggcc
     2581 gcgatggcct gcgcctcgtc catcgctccg gccgggcctt tctcctcgcg caggtggcag
     2641 cggacgatct gcgggtgatc cgggccgggg ccggtcctgg tccatcccgg atcctcgcgg
     2701 aagcagcgct cgaacgcgaa atcgcagcgc tcggcgaagc ggcactgccg gaagtccgcg
     2761 gtgagcggcg gcacctgtcc cttgatcacc gacagctcgc cgccgcgctt gcccgcatcg
     2821 ggcagcgcgg cgaagagctt gcgcgaatac gggtgctggg gcgcgctgaa gaagcgctcg
     2881 cggtcggcga cctcgaccag gtggcccgcg tacatcaccg cgacgcgctg cgccatctgc
     2941 gacacgatcc cgaggtcgtg cgtgatgagc agcatcgaca tgccggtctt cttctgcagg
     3001 tcgcgcagca gctcgaggat ctgcgcctgg atcgtgacgt cgagcgcggt ggtcggctcg
     3061 tcggcgatca gcaggtcggg gccggcggcc agcgccatcg cgatcatgac gcgctgcttc
     3121 atcccgccgg agagctggaa cgggtacgcg gtcgcgcggc gcccggcgtc cgggatgccg
     3181 accgactcga tcagctcgac gacgcgggcg tcgatcgcgg cgcctttcag cggcgtatgc
     3241 cgctggagca cctcgcggat ctggtagccg accgtgagca ccgggttgag gctggtcccc
     3301 ggctcctgga aaatcatcgc cactcgccgc ccgcgcacca cgcgcatcgc cgcctcgggg
     3361 agcgcgagca gatcggtgcc ctcgagctcg accgtgccgg cggcgatgcg gccggtctcg
     3421 ggcagcaggc gcatgatcga aagcgccgtc atcgacttgc cgctgcccga ctcgcccacc
     3481 agcgcgaagg tctcgccgcg gcggatctcg aggtcgatgc cgtcgacggc gcgcacgggc
     3541 tcgccggcgc cgtagaggac ggtcttcagg ccctggacgc gaagcagcag ctcgctcata
     3601 ccggcagcaa ccctccgacc cgggtgcgcg gatcgaaagc gtctcgcacg gcgtccgcga
     3661 acaggttcgc cgccagcacc agcgcgaaca tgaacacgaa cgccgccccg agcgtccacc
     3721 agaccatggg ctcgcgcgac atctccaggc gcgcggcgtt gatcatggtt ccgaagctga
     3781 tcatcgacgg gtcgacgccg acgccgacgt aggacagcac ggcctcggcg agcaccagcc
     3841 cggagaagtc gatcgccagc gagatgagca cgatgtgcat cacgttcggc agcacgtggc
     3901 gcgtcatgat gcgcgtgtcg cgcacaccga aggcctgcgc cgcctgaacg aattcgagct
     3961 cgcgcagctt cagcgtctcg ccgcgcagca gccgcgcgag ggtcgtccag ccggtgatgc
     4021 cgagcacgcc gcacagcacg agcaggcgca cgtcggcgcg ctgcaccgac gtcgggaaca
     4081 ggccggcgtg ctggtcgagg aacacctgcg ccaggaggat gaatgcggcg atcagcagaa
     4141 cgccggggat cgagttgatc accgtgtaga cgaactggat gacgtcgtcg atccagccgc
     4201 ggaagtatcc cgccatgatg ccgagagcga tcgcgaacgg cagcagcatc agcgtcgtca
     4261 ccgtgccgat gacgagcgcc gtcctcacgc tcttcatcgc caggtacagg acgtcccgcc
     4321 cgaccttgtc cgtgccgagc acgtggtagt acccgcacag cgcgagcatc gggcccgcga
     4381 gcgccagcag cgcgcccgcc gtgagcagca ccgcggccca cgggaagccc gtgccgccgc
     4441 tccagacggc gcgccaggaa tgccgccgcg acaccagcgt gccgagcccg gcccagacga
     4501 gaaccgcgac cgcgagcccg ccggccgcgc gcagcgcgat gtcgcgcccg cggtcggact
     4561 cctccttcag gtgcgcgccg ccccaggcca gccgcgggta ctcccgcact tgcagcgtct
     4621 tgccgcccgg cccgggcacg tcgagcgtct cgcgcgcgaa cgcgcgcgtc gcgagcggcg
     4681 ccgaataagt gcgctcgcgg tgcgcccaca acggcgtcgc cagcgcgtcg aacaccgagc
     4741 gtacctcggt cgagtacgcg gtttccccgc gctcgccctt gcgatcgagc gccggacgat
     4801 agtgcaacga atcgagcagc ccgatggcga cgtagacgac gaggaccacc agcgccgcca
     4861 tccccgccgc gctctgcgtc acgcggcccc acgggcgccg caggtgcggc tgccggcggg
     4921 cgtaccagga atacaaggcc agcgccgcga ccagcacgaa taccagcgcg tcggtccaga
     4981 ggatcaccgg catcggcgag gcctcattcg aaccgcaccc gggggtccgc caccgtatag
     5041 gaaatatcgg tgagcagcaa tccgacgatg tagagcacgg cgccgaggaa caccatcgag
     5101 cgcaccacga ggaaatcctg gttctggatg gcgtcgatcg tgtagctgcc gagccccggg
     5161 attccgaaga acgactccgt gatcagcgcg ccgaggaaca gcgtcggtat gacgacgacg
     5221 gcacccgtca ggatcgggat caggccgttc ttgagcacgt gccggaacat cacgacggtc
     5281 tcggccaggc ctttggcgcg cgcggttcgc acgtagtcct tgccgatctc ctcgagaaag
     5341 atcgtgcggt accagcgagc gccggagccg atgccgctca cggcgccgat cgcgaccggc
     5401 agcacgagga acttgaacgc atcgggcccc gaggagtagc ccgagatcgg gacgaggttc
     5461 cagatcttcg agaacacgta ttggccgccg atgatgtaga acaggctcga gatcgacatc
     5521 atcgcgacgc acaggaccac gccccagaaa tccaggtagg tgccgcggaa gaacgccatc
     5581 agcagcgcga aggtgatgtt gaccgcgagc ccgaccagga acaccggcag ctgcaccgcg
     5641 aggctgggcc acatccgggt cgagatctcg tggcggatcg agcggccgtc gtcggcgttg
     5701 ccgaagtcga atacgaaaag ccgcacggac ttctcgaaga agatggtgct cgtgacgcgc
     5761 gcgagcccgg tggcgcgatc gttgtaccac gtcggcttgt cgtagccgtg ctccgccttc
     5821 cagcgctcca ccgcctcggc cgtgatgcgc cgggcgccga gctgcagccg agccatgtcg
     5881 tccggggtgt tgagcttgaa gaacaggaag aacgtgagga ggttcacccc gatcaggatc
     5941 gggatcgcgt agagcgcgcg ccggacgata tacgcgatca cgcggcagtc tgtcggacct
     6001 gcgccgcggg gcgggcgacg atccgctcgc ggcggcgata gctcatccac gccgggacgc
     6061 tgcccagcac cagggcgagc aggatgagcg cgagcggcca gacgtccggc cggttccatt
     6121 cccggcgctt cgcctcgcgc agcgccgcgt cgacgcggat gtacttgagg ccgttgcgcg
     6181 ccatcttgtt cggcttgctg ttgtgcaccc aggcgtggga gagcacgtag tccttgggat
     6241 ggaagcccca gacccagggc gcgtcgtcgc gcacgagctt caccatctcg tcgatgatcg
     6301 cctgccgctt cgggccgttc tccatgttct tcatctgctc gaacagccgg tcgaagaccg
     6361 ggttggagta gcgcgccttg ttctcgccgt cctgcgtgcc gttcgggccg tagagcagga
     6421 acatgaagtt ctcgggatcc gggtagtcgg cgttccaccc gaggaaaaac atctgcgtgt
     6481 tgccggagcg gatcttgtcc tggaagcggt tgtagtcggt cgagcggatc tcgagctgca
     6541 cgtcgatctt ggcgaactgc cgacggtacc agttgagccg cgacgcgtcg tcgggcccgc
     6601 gctcggtggt gtcgaggtac aggaccagcg gctccccggt cttcgcgtcg cggccgtccg
     6661 ggtagccggc ctcggcgagc agcttcttcg cggcctccac gcccttgcgc cgcggcttgc
     6721 catcggggcc gcgctcgtag acgaccgggt tgtattcggc ctcgccctcg tgatagccga
     6781 agatcccggg cggcaaggga cccatgcccg ggagcccgcg gccgttcgcg aagatggaga
     6841 tgtactcctc ccagtcgatc gcgatcgaga ccgcctggcg cagcttgcgc gcccgctcgc
     6901 gcgcgcgtgc gtcgcccttg ttccccacga cgggatcgag gaagttgaac gcgaggtaga
     6961 aggtgctggt cgcgaccgag gtgcgcaggc ggatgccgcg ctcctccatc tcgggcgaaa
     7021 gcgtcgcgtc gccgccgacg ctgatgcgca ccgcctggtc gaaattgtcc gagccgatgc
     7081 ccgcggcgtc gtagtagccc tgaaggaact tgttccagag cgggatgctc tccttctcgc
     7141 gcgtgaacac gatgcgctcg ttcagcggca ggcgcttgcc cgcgtcggcg agcagccccg
     7201 cgccggcgtc gccgggctcg ccctccgacg gataggcctc gccgtggaag ttcgggttcg
     7261 cggcgagcac catgcgcgag ttcgggtcgt tctcggcgag catgtaaggc ccggtgccga
     7321 ccgggtacca gtcgagcgtg aggttgcgcc cttcgctcat cccgcgctgc gagtagaagc
     7381 ggtccgcctc ccacggcatg ggcgcgaaga acggcatcgc gagccagtac acgaactgag
     7441 ggtacttgcc cttgatgcgc acgcggtagg tgtagcggtc gacggcgtgc acgccggaga
     7501 gctcgtagcc gcgcaggtcg agccacggca gctgggtgtc ggcggcgccg tagcgctcgc
     7561 ggtgcgcggc ggcgatgacg gcgttgtcgc gccgcaggcg gtcgccgaga tccttcagcc
     7621 cgacgatgta gtcggtcatg tggccgtaaa tcggcgagac gatccgcgga tgcgcgagcc
     7681 gcttgatctg gtagacgtag tcttcggcga ccagctcgcg cgagccggtc ttcgggaagt
     7741 cggagagctt gtacttcgag cgcacgtcgc cttccgcgag cgacaggtac agcggctcgc
     7801 cgcggtcgtc ggtcgcgaac gccgggtgcg gctggtacat gatccccggc ttgatgtgga
     7861 tgtcgtactc gctgtacgcg acgcggtccg aatcggccgg gagcgggcgg ccgttcgcgt
     7921 cgaggtagcg cggcttcggg acctcggtcg cagtcgtagc ctcgagctcg taagggcgct
     7981 tcaagtagtg gtactgcagc ggcggctcgt agatctcctg gatgatgtcg tactcgtcgg
     8041 acgtgtacga ctgcgccggg tcgaggtgct tcggccgctc gatgaagaag gtgtagagga
     8101 tgtcgcggcc gcgctcgtcg gcgggatacg gatcgttcca gacgcgcccg cagccggtca
     8161 ggccgcacgc cagcagcgcc aggagaacca actgccggag aaacggcccg cgcacgaccc
     8221 gagtttagcg gaagcggccg cgcaccgtca cccgggcgcc gcgcgggcgt ccgctataat
     8281 cgggcgctct ccctgaggaa tccgcgatgg cgctgctcga aggcaaacgc atactcgtca
     8341 ccggcctgct ctccaaccgc tcgatcgcct ggggcatcgc gaaggcggcg aagcgcgagg
     8401 gcgcgcagct cgccttcacc taccagaacg agcgcttcaa ggaccgcgtc accgagatgg
     8461 ccgccgagct gggcagccgc ctggcggtgc cctgcgacgt cgcgagcgac gacgagatcg
     8521 cgcgcctgtt cgagtcgctg cgccgtgaat gggacggcct cgacggcgtg gtgcacgcga
     8581 tcgccttcgc gccgcgcgag gcgatcgccg gcgacttcct cgacggcttc tcgcgcgagg
     8641 cgttccgcgt cgcgcacgac gtcagcgcct acagcttcgg tgcgctggcg aagggcgcgc
     8701 tgccccttat gcagggccgc cacggcgcgc tgctcacgct cacctatctc ggagcggagc
     8761 gcacggtccc gaactacaac accatggggc tcgccaaggc gagcctcgag gcgagcgtgc
     8821 gctatctggc cgcgagcctg gggcccaggg gcatgcgggt gaacgcgatc tcggccgggc
     8881 cgatcaagac gctcgccgcg gccgggatcg ccggtttcgg caagatcctg aagcacgtcg
     8941 aggagcacgc gcctctgcgg cgcaacgtga ccatcgacga cgtcggcaac gcggcggcct
     9001 tcctgctctc ggacctggcg gcgggagtca cgggcgaggt gctgcacgtc gacgccggct
     9061 tcagcaccac ggtgggcggc atgccgaccg aggccggcgc gtaagactct cgcgccgcta
     9121 ctgcggcttc ttctcgatcg cctcgcggtt gatctcgacg ccgacgtcgg accgcagtcc
     9181 cgtgaggaag gagcggaatt cctcctcgcc gttggcgcgg ccgagctcgc tctggacggc
     9241 gcgctggcgc gcctcgtcgg gctcgacgtc gatcaccttg cccacgcggt acagcgcgta
     9301 gcccgcgggc acctcgacgc ccacgtacgc gggcagcgcg gacgcgtccg cgctgaagat
     9361 gcgcgacacg gccgccgggg cgagacccgc ggtcttctcg cggctcaccg tcttcgccgg
     9421 cgcgaacgcg acgcccgcgg cgttgccctt cttcagctcg gcgagccgct cggcgccctt
     9481 cttcgcggcg atctcgcgcg cctgccgctg cgcgagcagg cgctggatgt ccgacttcac
     9541 cgcctcgaag ggacgctggc tggcggcctt gtgctcgacc atgcgcgcgg cgaccagcgc
     9601 gttcggagcc gcctcgaccg cctcgctgtt gcgcttgttc gtgatcacgt cctccgagaa
     9661 cagcgcctgc agcacgcgcg gcgcgttcag gacctgcacc ggcgccgact gccgcgtgac
     9721 gccgtcggcg cggcggatct gcaggtggaa cttgtcggcg gcaggcttga gcgagtcggg
     9781 ctgctcgtac acggtgttgc tgaacgactc cgccgcctcg gggtagcggc gctgcgccgc
     9841 ctggcgcttc agctcgcgct cgatctcggg acggacctcc tcgagcgggc gcttcttgcc
     9901 gggcttcacg cccgtgagct tgatgatgtg gaggccgaac tcggactcga ccagcccgct
     9961 gatctggttc ggcttcatcc tgaagacggc gtcctcgaac tgcggcacca tcatgccgcg
    10021 cgcgaaccag ccgaggtcgc cgcccttcgg cgccgagccc gggtcctccg aatacttctt
    10081 cgcgagcgcc gcgaaggcgc cgggcgactt gcgtacctcg gcgagcacct gctcggcctt
    10141 ggcgcgcacc ttcgccttgt cggcgtcggt ggcgttgggc ttgtacgaga gcaggatgtg
    10201 gctcgcctcg cgctgctcgg cctctccgta cttggcggcg ttctgctcgt agtagctctt
    10261 cacggcagcc ggatcgatcg tctcggcggc ggcgagcgcg tccaggtcga gcaccacgta
    10321 ctcgaccgag atctgctcgg gtacctggaa ctgcgcaggg ttctggtcgt agtacgcgcg
    10381 cacagcttcg gcgctcggct gcgcctcgcg cgccttcgga tccgccgcga tcacgctctc
    10441 ggcgacctcg cgctgctgct gcgagaggcg cgcgagacgc tgcgcgaccg ccttcgacgc
    10501 gatgcccgag tcggacagcg cacccgtcag ctgctggagc atcaggtcgc gccgaaccga
    10561 agcctcgaag ctcgccggcg aatacccttc cccgcgcagc agcgtttcgt accgggcgct
    10621 cgagaacttg ccgtcctcct ggaaggccgg gatcgaggtg atggcgtcgc gcagttgc
//
LOCUS       DATDMW010000131         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_536747, whole genome shotgun sequence.
ACCESSION   DATDMW010000131 DATDMW010000000
VERSION     DATDMW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536747"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /gene="glyS"
                     /locus_tag="VI319_05675"
     CDS             <1..990
                     /gene="glyS"
                     /locus_tag="VI319_05675"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY6863369.1"
                     /translation="LTGRIAHDLGIDPLPAERAAWLSKADLLTGMVGEFPELQGVMGR
                     YYARHDGEPAAVCEAIEQHYWPRFAGDRLPRDVMGSAVALADRLDTLIGFLGVGLAPT
                     GDRDPFGLRRLALGVIRILSEQKLRLDLNELLRVAHANYPKGRLLDTHEHGANRVPNP
                     IEVGFFITDRARSYLREAGYATLEVEAVLDLAPRPAEYVERLDAVRSFLKLPQAAALA
                     ESDKRIRNILTKSGGAGVMQQTNVALLQDEAEKRLLGTTRMLRQEVESMLQEEKFRDA
                     LLSTARIHEPVATFFEKVLVNASDEQLRMNRFALLHEVIGLTNRVANISKLAA"
     gene            987..1520
                     /gene="gmhB"
                     /locus_tag="VI319_05680"
     CDS             987..1520
                     /gene="gmhB"
                     /locus_tag="VI319_05680"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEY6863370.1"
                     /translation="MKLVILDRDGVINQDSDEYIKNPDEWIPIPGSVEAIARLTHCGW
                     RVVVASNQSGVARGLFDMATLNAINAKMHRAVNQAGGRIDAVFYCPEHPDAASPFRKP
                     EPGMFLAIAERFNVPLVDVPVVGDSESDLRAAIAAGARPILVLTGKGQRTRANGILPS
                     GTTVYPDLAAVADALCP"
     gene            1511..2230
                     /locus_tag="VI319_05685"
     CDS             1511..2230
                     /locus_tag="VI319_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY6863371.1"
                     /translation="MPVIVLRSLAFALGLVLITPPYSLLALATFPLPRLLRYRIITGW
                     SRTVIWLARIVLGIRYSVRGRERLPAHPAVILAKHQSAWETLAFQAIFPPQVLVVKRE
                     LLWIPFFGWGLALASPIAIDRSRGPSALKRMAELGRRRLAQGFWITVFPEGTRTRPGE
                     RRPYHVGGAWLAVQCGAPVVPVAHNAGLLWGRNAFLKRPGTVTVEIGPTLQSTGADPH
                     ALNAAVEEWIESRMTALGPSH"
     gene            2248..2631
                     /gene="gloA"
                     /locus_tag="VI319_05690"
     CDS             2248..2631
                     /gene="gloA"
                     /locus_tag="VI319_05690"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY6863372.1"
                     /translation="MRILHTMLRVGDLDRSLRFYTEVLGMRPLRRNDYPDGKFTLAFV
                     GYGAESETAVLELTHNWGVEKYELGTAYGHVAIAVDDARKACEEVKERGGKVTREAGP
                     MKHGTTVIAFVEDPDGYKIELSERK"
BASE COUNT          428 a          956 c          934 g          414 t
ORIGIN      
        1 ctcaccgggc gcatcgcgca cgatctcggg atcgatccgc tgcccgccga gcgcgcggcg
       61 tggctgtcca aggccgactt gctcaccggc atggtgggcg agtttcccga gctgcagggg
      121 gtaatgggcc gttactacgc gcgccacgac ggcgagcccg cagccgtgtg cgaggcgatc
      181 gagcagcatt actggccgcg cttcgcgggc gaccggctgc cgcgcgacgt catggggtcg
      241 gcggtcgcgc tcgccgatcg tctcgacacg ctgatcggct ttctcggcgt cggactggcg
      301 ccgaccgggg acagagaccc gttcgggctg cgtcgcctcg cgctcggcgt catccgcatc
      361 ctgtccgagc agaagctgcg gctcgacctg aacgagctgt tgcgcgtcgc gcacgcgaac
      421 tacccgaagg gccggctgct cgatacgcac gagcacggag cgaaccgggt cccaaacccg
      481 atcgaggtcg ggttcttcat caccgaccgg gcgcgcagct acctgcgcga ggccggctac
      541 gcgacgctcg aggtggaagc cgtgctcgac ctcgcgcccc gcccggccga gtacgtcgag
      601 cggctcgacg cggtgcgcag cttcctcaag ctcccgcagg ccgcggcgct cgccgagtcc
      661 gacaagcgca tccgcaacat cctgacgaaa tccggcggcg ccggcgtcat gcagcagacc
      721 aatgtcgcgc tgctccagga cgaagccgag aagcggctcc tcggcaccac gcgcatgctg
      781 cgccaggaag tcgagtcgat gctgcaggag gagaagttcc gcgatgccct gctgtcgact
      841 gcgcgtatcc acgagccggt cgcgaccttc ttcgagaagg tcctcgtgaa cgcgagcgac
      901 gagcagctgc gcatgaaccg cttcgcgctg ctgcacgagg tgatcggcct gaccaaccgc
      961 gtcgccaaca tctcgaagct cgcggcatga agctcgtcat cctcgatcgc gacggcgtca
     1021 tcaatcaaga cagcgacgag tacatcaaga atcccgacga gtggatcccg attccgggca
     1081 gcgtcgaggc gatcgcgcgc ctgacgcatt gcggctggcg tgtcgtcgtc gcgagcaatc
     1141 aatcgggggt cgcgcgcggc ctgttcgaca tggcgacgct gaacgcgatc aacgccaaga
     1201 tgcaccgggc ggtgaaccag gccggcggac gcatcgacgc cgtgttctac tgccccgagc
     1261 accccgacgc ggcctcgcct ttccgcaaac cggagcccgg catgttcctc gcgatcgccg
     1321 agcgcttcaa cgtcccgctg gtcgacgtgc cggtggtggg cgacagcgaa agcgacctgc
     1381 gcgcggcgat cgcggccggc gcccggccga tcctggtcct gaccggcaag ggccagcgca
     1441 cgcgcgcgaa cggcatcctg ccctcgggca ccacggtcta cccggatctc gcggcggtcg
     1501 ccgacgccct gtgcccgtga tcgtgctgcg ctcgctcgcc ttcgcgctcg ggctcgtgct
     1561 gatcacgccg ccgtactcgc tgctcgcgct ggcgactttc ccgctgccgc gattgctccg
     1621 ctatcgcatc atcacgggct ggtcgaggac cgtgatctgg ctcgcgcgca tcgtcctcgg
     1681 catacgctac agcgtgcgcg gccgcgagcg gctgcccgcg catccggcgg tgatcctcgc
     1741 gaagcaccag tcggcgtggg agacgctcgc cttccaggcg atctttccgc cgcaggtgct
     1801 ggtcgtgaag cgagagctcc tgtggatccc attcttcggc tggggtcttg cgctcgccag
     1861 cccgatcgcc atcgaccgca gccgcgggcc ctcggcgctg aagcgcatgg cggagctcgg
     1921 ccggcggcgg ctcgctcagg gcttctggat cacggtgttc cccgagggca cgcgcacgcg
     1981 gccgggtgag cgcaggccct accacgtcgg gggcgcctgg ctcgcggtgc aatgcggggc
     2041 accggtcgtt ccggtcgcgc acaacgcggg cctgctctgg ggaaggaacg ccttcctgaa
     2101 gcgcccgggc acggtgacgg tggagatcgg tccgacgctg cagtccacgg gcgccgaccc
     2161 gcacgcgctg aacgccgcgg tcgaggagtg gatcgaatcg cggatgacgg cgctcgggcc
     2221 gtcgcactga tcacgatagg agagaccatg cgcatcctgc acaccatgct ccgggtcggc
     2281 gacctcgatc gttcgctgcg gttctacacc gaagtcctcg gcatgaggcc gctccggcgc
     2341 aacgactacc cggacggcaa attcacgctc gctttcgtcg gctacggcgc cgaatcggag
     2401 accgcggtgc tcgagctgac ccacaactgg ggcgtcgaga agtacgagct cggcaccgcc
     2461 tacggccacg tcgcgatcgc ggtcgacgac gcgcgcaagg cgtgcgaaga agtgaaggag
     2521 cgcggcggga aggtcactcg cgaggcgggt cccatgaagc acgggaccac ggtgatcgcc
     2581 ttcgtcgagg accccgacgg ctacaagatc gagctgagcg agcggaagta gctcagcgcg
     2641 gcgccggcgc cacgcctcgg aagcggcgga agttgttcgg ctggccgatc agcgccttgc
     2701 cgaactcctc gagcagcgct tcgttgatct cc
//
LOCUS       DATDMW010000132         8039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_661260, whole genome shotgun sequence.
ACCESSION   DATDMW010000132 DATDMW010000000
VERSION     DATDMW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661260"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VI319_05695"
     CDS             complement(<1..441)
                     /locus_tag="VI319_05695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6863373.1"
                     /translation="MGSPKHGDTALLLAVLLAILNNIGLKGSKMLVALYALDLGASPF
                     AIGALVSMYAIFPLVLAVHAGGVSDRFGPRRPMMLGSFGVATGLMLPVLFPALPTLYF
                     ACALIGASSLFFQVSVQNLVGSLGDASERTANFGTFSLGASISGF"
     gene            510..1046
                     /locus_tag="VI319_05700"
     CDS             510..1046
                     /locus_tag="VI319_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY6863374.1"
                     /translation="MNPGRNDPCPCGSGMKYKKCCGAVVPIQSSPQARACGTCTACCD
                     GWVAGTIRGHEMRPGVRCHFAGDGCCTIYEERPVSPCRNFVCGWLMPGSPFPEEFRPD
                     RLGVMIIQLAWRGKPAYLLRSAGRDPDEPLLEWMRAFSVRTGRPFFYEQAGEKLGFGP
                     AAFQQDMLQKAERGEAMW"
     gene            1192..2247
                     /locus_tag="VI319_05705"
     CDS             1192..2247
                     /locus_tag="VI319_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6863375.1"
                     /translation="MERRSRGAWPLLSVFSLALATSAPAAALAQGGNAALYTYKGADR
                     EQRLIEGAKKEGSVVLYTSLATTESVPLSQAFEKKYGVKVELWRATSDKVVQRALTEA
                     RARRHAVDVVETNGPELEMLAREKLLSRFESPYLEDLPPAAVPAHRLWVSDRLNFFVV
                     AYNTDKVKRAELPKTYDGFLDPKGKGRIGIEATDQEWLATIVKLWGEPKGMDFFRKLA
                     AMRPDVRKGHVLLSELIAVGEVPVGLTVYNANAESMKRRGGPIDWAPVQPVVARPQAI
                     AVFAEAPHPHAALLLADFVLSPEGQQLFLSMGRVPASIKVKSPLNNFPYTLVDPITVL
                     DESGKWEKIWNDLFIKR"
     gene            2255..3055
                     /locus_tag="VI319_05710"
     CDS             2255..3055
                     /locus_tag="VI319_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HEY6863376.1"
                     /translation="MSVMPHDLPGLLLLVFLLGVKHGVDPDHLATVDGLTRFNLAARP
                     GLARWSGCLFSLGHGLMVMAVAALVALSAGAWSAPGWLEPVGAWISIAFLVALGALNL
                     AAVLRTPRDRVARAAGLKGRWIGRLTETSHPVLIASIGAAFALSFDTMSQAALFSLSA
                     SGAAGWAFAAFLGFVFTSGMIVADGANGLWVSRLLARADRRARVASRVLGLSIAFLSL
                     AIAALGVAKAASPSAAALAEAGGLALGLGVVVIVLASYALALRLSQEG"
     gene            3056..3871
                     /locus_tag="VI319_05715"
     CDS             3056..3871
                     /locus_tag="VI319_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY6863377.1"
                     /translation="MDLSVKPVRIKTRLSSVANALRLMKAFSDDDTEIGISALAQRLG
                     LAKSTVHRLAATLITEGMLEQDRETGKYRLGLAVFELGALVRRKMDVSTEARPYLKAL
                     RDTTGETIHLAVLDHASVLYINKMESRQAIRMSSNIGGRAPAYCTSDGKALLAFQPED
                     FVAGMLKAGMPARTPNTITDVKGLLRDLSSIRARGYAVDNEESELGMRSVAAPIRTHS
                     GGVIASLSIAGPTHRVSKKTLAVYAREIVQAAEAISQRLGYQPARVLAPLKSA"
     gene            3882..5627
                     /locus_tag="VI319_05720"
     CDS             3882..5627
                     /locus_tag="VI319_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY6863378.1"
                     /translation="MAIAGELTAGRGMPPAERRGFDPKWIFIAVSVAIVAYLGLVPLV
                     FLLWQSFFTPQTAAKAAQFTFDNYVQAYTSLETFRLFANSLQFAVGSAIFAFVLGTLL
                     AWMNERTNTPFKGLFFALSIIPLIIPGILFVVAWILLGSPKIGLINLFLQKWLGTDFV
                     FINVYSLWGMMFVDGLHYSPMAFLLMTAAFRSMDPALEESAMMSGANVLQVAWRVTLR
                     LSWPAVFATLLILFVRALESFEVPALLGLPVGIEVFTSSIYRAIHQYPSQIGLASSYA
                     VTLLLITSVGVYFQSRLSSQGSKYSTMTGKGFRPRTIDLGAWRWAAAGLFIAYFLLIV
                     ILPFLVLLWSSFQRFYSVPSMQALHNLTLDAYRFVFTYPTLATSVWNSLLLAFGSATI
                     IMLVTSVICWIVVKTKFPGRWLLDNLASLPMVFPGLVLGLSIMIFYLNVDIGIYGTIW
                     IMFLAYITRFLPYGLRYNTTSMLQIHKELEESAAMSGASWSTTFWRIILPLLKPGLLA
                     GWIYIVIVSIRELSASILLYSPGTEVVSITIWELWENGQYVELSALGVLLILALFVLV
                     MLAQMVARRFGMKEA"
     gene            5633..6748
                     /locus_tag="VI319_05725"
     CDS             5633..6748
                     /locus_tag="VI319_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863379.1"
                     /translation="MLKVDALYTEYPNERGQVVKAAQNVSFEVPQGKLFTLLGPSGCG
                     KTTTLRSIAGLERPRSGEIAVDGRVVYSSQKKTFVAPNKRNFGMVFQSYAIWPHMTVY
                     ENAAFPLQVGNGRHGRQEIRDKVMRVLSTVALDHLADREATKLSGGQQQRLALARALA
                     MEPQLLLLDEPLSNLDAKLRERMRFELKRLQRELRLTTVYVTHDQGEALALSHEIAVM
                     NEGQIVQVGAPRDIYERPQNRFVADFVGSANFLDATVAGKGDARNGWHVRTAIGLLEC
                     TAVDALREGDTVAVSVRPEDVHMSAERQGERNVIVGTVDAKIFLGECLEFQVKVDGTM
                     LLARVHPSFSPPVGTQVHLRIDPEKCIAIPEEKRKAA"
     gene            6751..>8039
                     /locus_tag="VI319_05730"
     CDS             6751..>8039
                     /locus_tag="VI319_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY6863380.1"
                     /translation="MANDARPDPKSPKKSPYAPDRRWASDVIVDLLQRYDLKYAALNP
                     GASYRGLHDSIVNYGGNKPVMMLCQHEETAVQIAHGYAKASGKPMVAILHNLVGLLHA
                     NLSIYYAYIDRAPVFIVGATGPMDETKRRPRIDWTHTALVQGNAVRDYTKWDYQPHVI
                     DGVPESFARAYAVMMTEPRGPIYMCYDAWLQEQPLDHDVPLPPETAAAVPAPLGPDPE
                     ALARAADLIAKAKRPAIIAEFVGRDPKGFHALVEFAETIGAPVYDVNARLNFPTEHPL
                     NLSMVKDIFRDADLVLTLDVRDWERPTTELVSTTRKVTSVVPEGALWVDIGFGDLELS
                     SWAMDYQRLMHAHLRVLADTTVAIPVLTELLKRRIAGDGALTKTIDERTKRWHELHRK
                     TRAQWAAEAKEDWDASPIPLGRLASEVWDAIKGEDWVL"
BASE COUNT         1253 a         2770 c         2740 g         1276 t
ORIGIN      
        1 aaacccggag atcgatgcgc ccaggctgaa ggtgccgaag ttcgcggtgc gctcgctcgc
       61 gtcgccgagc gagccgacca ggttctgcac cgagacctgg aagaagagcg agctcgcgcc
      121 gatcagcgcg caggcgaagt agagcgtcgg cagcgcgggg aagagcaccg gcagcatcag
      181 tcctgtcgcg acgccgaacg atcccagcat catcggccgg cgcggcccga agcggtccga
      241 cacgccgccc gcgtgcaccg cgagcaccag cgggaagatc gcgtacatcg agaccagagc
      301 gccgatcgcg aacgggctcg cgccgagatc gagcgcgtag agcgccacca gcatcttgct
      361 gcccttcagg ccgatgttgt tgaggatggc gagaaggacg gcgaggagca gcgccgtgtc
      421 gccatgtttc ggggacccca ttcggagcta acgcggaaga agatcgaagc ttgacatggc
      481 gagatgcgct tcgtagtgta cccgcgagca tgaatcccgg cagaaacgat ccgtgtccct
      541 gcggcagcgg catgaaatac aagaagtgct gcggcgccgt cgtcccgatc cagtcctcgc
      601 cgcaggcgcg cgcctgcggc acgtgcacgg catgctgcga cggctgggtc gccggcacga
      661 tccgcgggca cgagatgcgg cccggcgtgc gctgccactt cgccggcgac ggctgctgca
      721 ccatctacga ggagcgcccg gtctcgccgt gccggaactt cgtctgcggc tggctcatgc
      781 cggggagccc cttccccgag gagttccgtc ccgaccggct gggcgtcatg atcatccagc
      841 tcgcgtggcg cgggaagccc gcgtacctgc tgcgctccgc gggacgcgat cccgacgagc
      901 cgctgctcga atggatgcgc gcgttcagcg tacgcaccgg gcggccgttc ttctacgagc
      961 aggcgggaga gaagctcggc ttcggaccgg cggcgttcca gcaggacatg ctccagaagg
     1021 cggagcgcgg ggaggcgatg tggtaggcgc agtccggcct cgtccctgat tccccggggt
     1081 ttacccgcgc cgcgctaagg cgccgttccg atatatggaa cggggttcca tttgtccgga
     1141 aaatcccgat agaatccccg cgaggagaga gtcgaaggag gaagagacga catggagcgg
     1201 cgctcgaggg gcgcatggcc cctgctttcc gttttttcgc tggcgctcgc gacgtccgcc
     1261 ccggcggcgg cgctcgccca gggcggcaat gcggctctgt atacatacaa gggagccgac
     1321 cgcgagcagc gcctgatcga gggcgcgaag aaggagggca gcgtcgtcct ctacacgtcg
     1381 ctcgccacca ccgagtcggt gccgctgtcg caggcgttcg agaagaagta cggcgtcaag
     1441 gtcgagctgt ggcgcgcgac cagcgacaag gtcgtgcagc gcgcgctgac cgaggcaaga
     1501 gcgcgccgcc acgcggtcga cgtggtcgag accaacggcc ccgagctcga gatgctcgcg
     1561 cgcgagaagc tgctgtcgcg cttcgagagt ccctacctcg aggacctgcc gccggccgcg
     1621 gtccccgcgc accggctgtg ggtctccgac cgcctcaact tcttcgtcgt cgcctacaac
     1681 accgacaagg tgaagcgcgc cgagctgccg aagacctacg acggcttcct cgacccgaag
     1741 gggaagggcc gcatcggcat cgaggcgacc gaccaggagt ggctcgcgac catcgtcaag
     1801 ctctggggcg agcccaaggg catggacttc ttccgcaagc tcgccgcgat gcggccggac
     1861 gtgaggaagg ggcacgtgct gctctccgag ctgatcgcgg tcggcgaggt gccggtgggg
     1921 ctcaccgtgt acaacgcgaa cgccgagtcg atgaagcgcc gcggcggccc gatcgactgg
     1981 gcgccggtgc aaccggtggt cgcgcgcccg caggcgatcg cggtcttcgc cgaggcgccg
     2041 cacccgcacg cggcgctgct gctcgccgac ttcgtgctct cgcccgaggg gcagcagctg
     2101 ttcctgtcga tggggcgcgt cccggcgagc atcaaggtga agagcccgct gaacaatttc
     2161 ccgtacacgc tggtcgaccc gatcacggtg ctcgacgagt ccgggaaatg ggaaaagata
     2221 tggaacgacc tgttcatcaa gcgctgagcg cggcatgagc gtcatgccgc acgacctgcc
     2281 ggggctgctg ctgctggtct ttctcctcgg cgtgaagcac ggcgtcgacc ccgatcacct
     2341 tgccacggtc gacggcctga cgcgattcaa cctcgcggcg cggccgggcc tcgcgcgctg
     2401 gtccgggtgt ctgttctcgc tcgggcacgg gctgatggtg atggcggtcg ccgcgttggt
     2461 cgcgctgagc gccggggcct ggtcggcgcc gggctggctc gagccggtgg gggcgtggat
     2521 ctcgatcgcg ttcctggtcg cgctcggcgc gctcaacctc gccgcggtgc tgcgcacgcc
     2581 gcgcgaccgc gtcgcgcgtg ccgccggtct caaaggccgc tggatcgggc ggctgaccga
     2641 gaccagccat ccggtgctga tcgcctcgat cggggccgcg ttcgcgctgt cgttcgacac
     2701 catgagccag gcggcgctgt tctcgctgtc cgcgtccgga gctgccggct gggcgttcgc
     2761 ggcgttcctg ggattcgtct tcacctccgg catgatcgtc gcggacggcg ccaacggact
     2821 ctgggtgtcg cgcctgctcg cgcgcgccga ccgccgcgcg cgcgtcgcct cgcgcgtgct
     2881 cgggctctcg atcgcgttcc tgtcgctggc catcgcggcg ctcggcgtcg ccaaggccgc
     2941 gtcgccgagc gcggcggcgc tggccgaggc gggcgggctc gcgctcgggc tgggcgtcgt
     3001 cgtcatcgtg ctcgcgagct acgcgctcgc gctgcgcctg tcgcaggagg ggtagatgga
     3061 tctgagcgtc aagcccgtgc gcatcaagac gcggctgtcg tcggtcgcga acgcgctgcg
     3121 cctgatgaag gcgttctccg acgacgacac cgagatcggc atcagcgcgc tcgcgcagcg
     3181 cctgggcctc gccaagagca cggtgcaccg gctcgccgcg acgctgatca ccgagggcat
     3241 gctcgagcag gatcgcgaga ccggcaagta ccggctgggg ctcgccgtgt tcgagctggg
     3301 cgcgctcgtg cgccgcaaga tggacgtgtc gaccgaggcg cgcccgtacc tgaaggcgtt
     3361 gcgcgacacg accggcgaga ccatccacct cgcggtgctc gatcacgcga gcgtgctcta
     3421 catcaacaag atggagagcc gccaggcgat ccggatgagc tccaacatcg gcggccgggc
     3481 gccggcgtac tgcaccagcg acggcaaggc gctgctcgcg ttccagcccg aggacttcgt
     3541 cgccgggatg ctgaaggccg ggatgccggc gcgcacgccg aacacgatca ccgacgtgaa
     3601 ggggctgctg cgcgacctct cgtcgatccg ggcgcgcggc tacgccgtcg acaacgagga
     3661 gagcgagctc ggcatgcgct cggtcgcggc gccgatccgc acgcattcgg gcggcgtcat
     3721 cgcgtcactc tcgatcgccg ggccgacgca ccgcgtcagc aagaagacgc tcgccgtcta
     3781 cgcgcgcgag atcgtccagg ccgccgaagc catctcgcag cggctcggct accagccggc
     3841 gcgcgtgctc gcgccgctga agagcgcctg aggagaccgc catggcgatc gcgggtgagc
     3901 tgacggccgg ccgcggaatg ccgccggccg agcgccgcgg cttcgacccg aaatggatct
     3961 tcatcgccgt gtcggtcgcc atcgtcgcct acctcggcct ggtgccgctc gtgttcctgc
     4021 tgtggcagag cttcttcacg ccgcagacgg ccgccaaggc cgcgcagttc acgttcgaca
     4081 actacgtcca ggcgtacacc agtctcgaga ccttccgcct gttcgcgaac tcgcttcagt
     4141 tcgccgtcgg ctcggcgatc ttcgccttcg tcctcgggac gctgctcgcg tggatgaacg
     4201 agcgcaccaa cacgccgttc aagggcctgt tcttcgcgct ctcgatcatc ccgctgatca
     4261 tcccggggat cctgttcgtg gtcgcgtgga tcctgctcgg cagcccgaag atcggcctca
     4321 tcaacctgtt cctgcagaag tggctcggga ccgacttcgt gttcatcaac gtctactcgc
     4381 tctggggcat gatgttcgtc gacgggctgc actactcgcc gatggccttc ctgctcatga
     4441 ccgcggcgtt ccgctccatg gaccccgcgc tcgaggagtc ggcgatgatg agcggcgcca
     4501 acgtcctgca ggtcgcgtgg cgcgtgacgc tgcgcctgtc gtggcccgcg gtgttcgcga
     4561 cgctgctgat cctgttcgtg cgcgcgctcg agtccttcga ggtaccggcg ctgctggggc
     4621 tgccggtcgg catcgaggtg ttcacctcgt cgatctaccg cgcgatccac cagtatccgt
     4681 cgcagatcgg gctcgcctcg tcctatgcgg tgaccctgct gctgatcacc agcgtcggcg
     4741 tctacttcca gtcgcggctc tcgagccagg ggagcaagta ctcgaccatg acggggaagg
     4801 gcttccggcc gcgcaccatc gacctcggtg cctggcgctg ggccgccgcg ggcctgttca
     4861 tcgcctactt cctgctgatc gtgatcctgc cgttcctggt gctgctatgg tcgtcgttcc
     4921 agcgcttcta cagcgtgccg tcgatgcagg cgctgcacaa cctgacgctc gacgcgtacc
     4981 gcttcgtgtt cacctacccg acgctcgcga cctcggtctg gaacagcctg ctgctcgcgt
     5041 tcggcagcgc caccatcatc atgctcgtga cctcggtgat ctgctggatc gtggtcaaga
     5101 cgaaattccc gggccgctgg ctgctcgaca acctggcgtc gctgccgatg gtgttcccgg
     5161 gcctggtgct cggcctgtcg atcatgatct tctacctgaa cgtcgacatc ggcatctacg
     5221 gcacgatctg gatcatgttc ctcgcctaca tcacgcgctt cctgccgtac gggctgcgct
     5281 acaacacgac ctcgatgctg cagatccaca aggagctgga ggagtccgcg gcgatgagcg
     5341 gcgcgtcgtg gagcacgacg ttctggcgca tcatcctgcc gctcctgaag ccgggcctgc
     5401 tcgcgggctg gatctacatc gtcatcgtct cgatccgcga gctgtcggcg tcgatcctgc
     5461 tctacagccc gggcaccgag gtggtgtcga tcaccatctg ggagctctgg gagaacgggc
     5521 agtatgtcga gctgtcggcg ctcggcgtgc tgctgatcct cgcgctcttc gtgctcgtga
     5581 tgctggcgca gatggtcgcg cgccggttcg gaatgaagga ggcctgatcc ccgtgctgaa
     5641 agtcgacgcc ctctacaccg agtatccgaa cgagcgcggc caggtcgtga aggccgcgca
     5701 gaacgtctcg ttcgaagtgc cccagggcaa gctgttcacg ctgctcgggc ccagcggctg
     5761 cggcaagacc acgacgctgc gctcgatcgc gggcctcgag cgcccgcgct cgggcgagat
     5821 cgcggtcgac ggccgcgtcg tgtactcgtc gcagaagaag accttcgtcg cgcccaacaa
     5881 gcgcaacttc ggcatggtgt tccagtcgta cgcgatctgg ccgcacatga cggtgtacga
     5941 gaacgccgcg ttcccgctgc aggtgggcaa cggcaggcac ggcaggcagg agatccgcga
     6001 caaggtcatg cgcgtgctga gcaccgtggc gctcgaccac ctcgccgacc gcgaggcgac
     6061 caagctctcg ggcggccagc agcagcgcct ggcgctcgcg cgcgccctcg cgatggagcc
     6121 gcagctgctt ctgctcgacg agccgctctc caacctcgac gccaagctgc gcgagcgcat
     6181 gcgcttcgag ctgaagcggc tgcagcgcga gctgcgcctc accactgtct acgtgacgca
     6241 cgaccagggc gaggcgctcg cgctctcgca cgagatcgcc gtgatgaacg aggggcagat
     6301 cgtgcaggtc ggcgcgccgc gcgacatcta cgagcggccg cagaaccgct tcgtcgccga
     6361 cttcgtcggc tcggccaact ttctcgacgc gaccgtcgcg ggcaagggcg acgcgcggaa
     6421 cggctggcac gtgcgcaccg cgatcggcct gctcgagtgc accgccgtcg acgcgctgcg
     6481 cgagggcgac acggtcgccg tctcggtgcg gcccgaggat gtccacatgt cggccgagcg
     6541 ccagggcgag cgcaacgtca tcgtcggcac cgtcgacgcg aagatcttcc tgggcgagtg
     6601 cctcgagttc caggtcaagg tcgacggcac gatgctgctc gcgcgcgtgc acccgtcgtt
     6661 ctcgccgccg gtcggcaccc aggtgcacct gcgcatcgat cccgagaagt gcatcgcgat
     6721 tcccgaagag aagaggaaag ccgcctgacc atggccaacg acgctcgtcc cgaccccaag
     6781 agcccgaaga agagccctta tgcgcccgac cgccgctggg cctccgacgt catcgtcgac
     6841 ctgctgcagc gctacgacct caagtacgcg gcgctcaacc ccggcgccag ctaccgcggc
     6901 ctgcacgact cgatcgtgaa ctacggcggc aacaagcctg tcatgatgct gtgccagcac
     6961 gaagagaccg cggtgcagat cgcgcacggc tacgccaagg cgagcgggaa gccgatggtc
     7021 gcgatcctgc acaacctggt cggcctgctg cacgcgaacc tgtcgatcta ctacgcctac
     7081 atcgaccgcg cgccggtctt catcgtcggc gccaccggcc cgatggacga gaccaagcgc
     7141 cgcccgcgca tcgactggac ccacaccgcg ctcgtgcagg gcaacgcggt gcgcgactac
     7201 accaagtggg actaccagcc gcacgtgatc gacggcgtgc cggagtcctt cgcgcgcgcc
     7261 tacgccgtga tgatgaccga gccgcgcggc ccgatctata tgtgctacga cgcctggctg
     7321 caggagcagc cgctcgacca cgacgtgccg ctgccgcccg agacggccgc cgcggtgccg
     7381 gcgccgctcg ggcccgaccc ggaggcgctc gcgcgcgccg ccgacctgat cgctaaggcg
     7441 aagcgcccgg cgatcatcgc cgagttcgtc ggtcgcgacc cgaagggctt ccatgcgctg
     7501 gtcgagttcg ccgagacgat cggggcgccg gtctacgacg tcaacgcgcg gctgaacttc
     7561 ccgaccgagc acccgctgaa cctttcgatg gtgaaggaca tcttccgcga cgcggacctg
     7621 gtcctgacgc tcgacgtgcg cgactgggag cggccgacga ccgagctggt cagcacgacg
     7681 cgcaaggtca cgagcgtcgt gcccgagggc gcgctgtggg tcgacatcgg cttcggcgac
     7741 ctcgagctct cgagctgggc aatggactat cagcgcctga tgcacgcgca tctgcgcgtg
     7801 ttggccgaca ccacggtcgc gatcccggtg ttgaccgagc tattgaagcg ccggatcgcc
     7861 ggcgacggcg cgctgacgaa gacgatcgac gagcgcacga agcgctggca cgagctgcac
     7921 cgcaagacgc gggcgcagtg ggccgccgaa gcgaaggagg actgggacgc gagccccatc
     7981 ccgctcgggc gcctcgcgtc cgaggtctgg gacgcgatca aaggcgagga ctgggtcct
//
LOCUS       DATDMW010000133         2247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_413846, whole genome shotgun sequence.
ACCESSION   DATDMW010000133 DATDMW010000000
VERSION     DATDMW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413846"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VI319_05735"
     CDS             complement(<1..595)
                     /locus_tag="VI319_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6863381.1"
                     /translation="MSAEALLERELDRFEQEHPRSRELANEAKGPLLSGVPMPWMIRW
                     PGGFPVFAAEARGARFTDVDGHEYVDFCLGDTAAMTGHSPEPTVRALREQAAKGITLM
                     LPSEDALWVGRELTRRFGLARWQFALTATDANRFAIRLARERTGRPKILVFNWCYHGS
                     VDETFAVIGPDGTAIERRGNIGRPVALAETTRVVESND"
     gene            complement(592..1770)
                     /locus_tag="VI319_05740"
     CDS             complement(592..1770)
                     /locus_tag="VI319_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6863382.1"
                     /translation="MHPRWLNRGVAGIGVASFFSDVSHEVPTALLPALLTTTLGAPAA
                     ALGTIEGIADGIAGAAKLAGGALADDPGRRRATAMGGYTATAVLSALIGAATAVWQVG
                     VLRAGAWLSRGLRTPSRNALLTDVVEPEVYGRAYGFERAMDNAGAIAGPLLALALISV
                     VGIREAILISVVPGLLAAVAILVAVRSAQRLEPHERPPLRIRIRPVLHGRLGRLLGAV
                     TAFELGNAAATLLILRATDLLTPAHGHDSAVRLAVVLYAVYNAAAMVISVPAGHTTDR
                     RGATAVLVVGAFAALAAFAGFALSGSSLALLAALFALAGTGKGAVETAQSAAVALLAP
                     PGLLGSAFGALATIQSFANLAASAVAGALWTLVSPRAAFLYLAGWAAVSVVALATVRR
                     "
     gene            complement(1752..>2247)
                     /locus_tag="VI319_05745"
     CDS             complement(1752..>2247)
                     /locus_tag="VI319_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863383.1"
                     /translation="QRQRVAIAAAIVLDPELLVADEPVSMLDVSVRAGVLGLLDRLRR
                     GGLGVLMITHDLSTAARFADRIAVMYLGRIVEEGPAAQVVDDPQHPYTQALLSVVPRR
                     DPRERTRPQILRGEAPDAVRIPAGCRFHPRCPIAIDDCRSVDPPLEPAGSSAAHRAAC
                     ILAG"
BASE COUNT          304 a          820 c          880 g          243 t
ORIGIN      
        1 ggtcgttgct ctccacgacg cgcgtcgtct cggcgagggc cacgggcctg ccgatgttgc
       61 cccggcgctc gatcgccgtc ccgtccgggc cgatgaccgc gaaggtctcg tcgaccgagc
      121 cgtggtagca ccagttgaag acgaggatct tcggccggcc cgtgcgctcg cgcgcgagcc
      181 ggatcgcgaa ccggttcgcg tccgtcgcgg tgagcgcgaa ctgccagcgc gcgagcccga
      241 agcgccgcgt cagctcgcgg ccgacccaga gcgcgtcctc gctcggaagc atcagcgtga
      301 tcccctttgc cgcctgctcg cgcagggcgc ggaccgtcgg ctcgggcgag tggccggtca
      361 tcgccgcggt gtcgccgagg cagaagtcga cgtactcgtg gccgtcgacg tcggtgaagc
      421 gcgcgccgcg cgcctcggcc gcgaagacgg ggaagccgcc cggccagcgg atcatccacg
      481 gcatcggcac gccgctcagc agggggccct tcgcctcgtt cgccagctcg cgggagcgcg
      541 ggtgctcctg ttcgaagcgg tcgagctcgc gctcgagcag cgcctcggcg ctcaccggcg
      601 caccgttgcg agggcgacca cgctcacggc tgcccagcct gccaggtaga ggaaggccgc
      661 gcgaggggag acgagcgtcc agagcgcgcc ggcgaccgcg gaggcagcga ggttggcgaa
      721 gctctggatc gtcgcgagcg cgccgaaggc cgagccgagc aggccgggcg gcgcgagcag
      781 cgcgacggcg gcgctctgcg cggtctccac cgcgcccttg ccggtgcccg cgagggcgaa
      841 gagcgcggcc agcagcgcga ggctcgagcc cgagagggcg aagccggcga acgcggcgag
      901 ggcggcgaag gcaccgacca cgagcaccgc ggtcgcgccg cggcggtcgg tcgtgtggcc
      961 cgcggggacg ctgatcacca tcgcggcggc gttgtagacc gcgtacagca cgaccgccag
     1021 ccggaccgcg gagtcgtggc cgtgggcggg cgtcaggagg tcggtcgcgc gcaggatcag
     1081 cagcgtcgcg gccgcgttgc cgagctcgaa cgccgtgacc gcgccgagga ggcggccgag
     1141 ccgcccgtgc agcaccggcc ggatgcggat gcgcagcggg gggcgctcgt gcggctcgag
     1201 ccgctgcgcc gagcggacgg cgacgaggat cgccaccgcg gccagcaggc ccgggacgac
     1261 cgagatcagg atcgcctccc ggatgcccac gaccgagatc agcgcgagcg ccaggagcgg
     1321 cccggcgatc gcgcccgcgt tgtccatcgc ccgctcgaac ccgtatgcgc gaccgtagac
     1381 ctccggctcg acgacgtccg tcaggagcgc gttgcgcgac ggcgtccgca gcccgcgcga
     1441 gagccacgcg cccgcgcgca ggacgccgac ctgccagacc gcggtcgcgg ctccgatcag
     1501 cgcgctcagg acggcggtcg cggtgtagcc gcccatcgcc gtcgcacgac ggcggccggg
     1561 gtcgtccgcg agggcgccgc cggcgagctt ggccgcgccc gcgatcccgt cggcgatgcc
     1621 ctcgatcgtg ccgagcgccg ccgcgggcgc gccgagcgtc gtcgtcagca gggccggcag
     1681 gagcgccgtc ggcacctcgt ggctcacgtc cgagaagaag ctcgcgacgc cgatcccggc
     1741 gacgccgcgg ttcagccagc gaggatgcac gcggcccggt gagcggcgct cgaccccgcc
     1801 ggctccagcg gagggtcgac cgagcggcag tcgtcgatcg cgatcgggca cctcgggtgg
     1861 aagcggcagc ccgcggggat gcgcactgcg tcgggcgcct cgccgcgcag gatctgcggc
     1921 cgcgtgcgct cgcgcgggtc gcggcgcggg acgacggaca gcagggcctg ggtgtacggg
     1981 tgctgcgggt cgtcgaccac ctgcgcggcc gggccctcct cgacgatccg ccccaggtac
     2041 atgaccgcga tgcggtcggc gaagcgggct gcggtggaga ggtcgtgcgt gatcatcagc
     2101 acgccgaggc cgccgcggcg aagccggtcg agcagcccga gcacgcccgc gcgcaccgag
     2161 acgtcgagca tcgagaccgg ctcgtcggcc acgagcagct cggggtcgag cacgatcgcc
     2221 gcggcgatcg cgacgcgctg gcgctga
//
LOCUS       DATDMW010000134         3772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1410050, whole genome shotgun sequence.
ACCESSION   DATDMW010000134 DATDMW010000000
VERSION     DATDMW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410050"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VI319_05750"
     CDS             <1..1011
                     /locus_tag="VI319_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY6863384.1"
                     /translation="KHVEVAHELEATLEERNVAADDALDDAIGDDLLRLMFTACHPVL
                     STEARVALTLRLLGGLTTDEIARAFLAAEPAIAQRIVRAKRALAEAKVPFEVPGAGEL
                     TARLASVLEVIYLVFNEGYSATAGEDWMRPALCQDALRLGRVLAELLPNEPEVHGLVA
                     LMELQASRAGARTGPEGEPVLLLDQDRSRWDRLLIRRGLEALERVRELKGESGPYALQ
                     AAIAACHARAATAKETDWVRIAALYAALAQSAPSPVVELNRAVAVAMAFGPEAGLEIV
                     DALASEPSLGSYHLLPSVRGDLLAKLGRKAEARVEFERAASLTQNARERKLLLERAAR
                     WS"
     gene            1116..1460
                     /locus_tag="VI319_05755"
     CDS             1116..1460
                     /locus_tag="VI319_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863385.1"
                     /translation="MPPKAVAAVVANGVRYQVPHFGALHGKAQNGGYVQAWDVKTGKL
                     LWDRMVYRVRYDENLEKDVQDDFISRISVRKGHLRVTTEGSEEFDMDLKSGVVRALTP
                     LGRNIEVADPKP"
     gene            1457..1858
                     /locus_tag="VI319_05760"
     CDS             1457..1858
                     /locus_tag="VI319_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863386.1"
                     /translation="MIWRKAVALWIVLLVFAIALAGVREAALAPALGDFRAHQVETVA
                     FCAVVAAVAFFGVRWMRATRRQGIRVGLLWLAMTLLFELGVFGLALGHPWPELLADYN
                     ILEGRLWPLVLVTELVAPAIFARRTEKEKTQ"
     gene            1855..2205
                     /locus_tag="VI319_05765"
     CDS             1855..2205
                     /locus_tag="VI319_05765"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY6863387.1"
                     /translation="MMAVIFEVWPAEGRAQDYFSIAGQLKADLEKIDGFVSIERFESL
                     TEKGKFLSLSFWRDEDAIRRWRNLEGHRAAQAKGRGGIFADYRLRVASVVRDYGMGER
                     AQAPKDSRAVHDTR"
     gene            complement(2210..3163)
                     /locus_tag="VI319_05770"
     CDS             complement(2210..3163)
                     /locus_tag="VI319_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY6863388.1"
                     /translation="MRRPALAACALVLLLAGCGTFAQPGPGSIAFALIGDAPYNTLEV
                     DEVDRLIDALNAQDLAFVIHVGDITAGHGPCNDEWFEARKRQFQRSKHPFVIVPGDND
                     WVDCHRSGKDPLERLDFFRKLFESGDESLGQQKIRLERQSSDRRFPEYREDMRWTAGD
                     VLFVGVNVQGSNNNVGRNPAMDREAEQRMAAVFDWLDEAVKLVEQRDLRGLVVFAQAD
                     PDFEKRVRRRPGVPDGWESFRNVLRAHALYLRKLGKPLLYVHGDRHHFVYDHPLADAS
                     GKTIGSFTRVEVWGSPFVRWIKAWIDPKAPDVFGVTPATGP"
     gene            complement(3186..>3772)
                     /locus_tag="VI319_05775"
     CDS             complement(3186..>3772)
                     /locus_tag="VI319_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY6863389.1"
                     /translation="LGGVGPSGGGWGAKHDEDGMSATVCMNDGDTHNHPIEQMEAKFP
                     LLFERHALREDSGGAGRHRGGLGTEQVVRARSTLVMNVQVDRMHCPPWGLEGGLAGAG
                     NQVTLRLGGVEKTDLPNGKVLTQRLQKGDAFTLRAGGGGGFGAPFERDPERVAEDVRQ
                     GYVSPRAARELYGVALDGNLAVDTAATAKLRKPS"
BASE COUNT          545 a         1279 c         1359 g          589 t
ORIGIN      
        1 aagcacgtcg aagtcgcgca tgagctcgaa gcgacgctgg aagagcgcaa cgtggctgcc
       61 gacgacgccc tcgacgatgc gatcggcgac gatctgttgc ggctgatgtt cacggcctgc
      121 cacccggtgc tgtcgaccga ggcgcgcgtg gcattgacgc tgcgcctgct cggcggcctg
      181 accacggacg aaatcgcacg cgcctttctc gccgcggagc cggccatcgc gcagcgcatc
      241 gtgcgcgcca agcgcgcgct cgcggaggcg aaggtgccgt tcgaggtgcc gggcgcaggc
      301 gagctcaccg cgcgcctcgc ctcggtgctc gaggtgatct acctcgtctt caacgaaggc
      361 tactcggcga ccgcgggcga ggactggatg cggcccgcgc tgtgccagga tgcgctgcgc
      421 ctcgggcggg tgctcgcgga gctgctgccg aacgaacccg aggtgcacgg cctcgtcgcg
      481 ctgatggaac tgcaggcctc gcgcgcgggc gcgcgcaccg ggcccgaagg cgagcccgtg
      541 ctgctcctcg accaggaccg ctcgcgctgg gaccggcttc tcatccgaag agggctcgag
      601 gcgctcgagc gcgtgcgcga gctgaaaggc gagtcgggtc cgtacgccct ccaggcggcg
      661 atcgcggcgt gccatgcgcg tgcggcgacc gccaaggaga cggactgggt ccggatcgcc
      721 gcgctctacg ccgcgctcgc ccagagcgcg ccttcacccg tcgtcgagct gaaccgcgcc
      781 gtcgcggtcg cgatggcgtt cgggcccgag gcggggctgg agatcgtcga cgcgctcgcg
      841 tcggagccgt cgctcgggtc ctatcacttg cttccgagcg tcaggggaga cctgctcgcg
      901 aagctgggcc gcaaggccga ggcgcgcgtg gagttcgagc gcgcggcgtc gctgacgcaa
      961 aacgcgcgag agcgtaagct gctgctcgag cgagccgcgc gatggtcctg aaaatgatgt
     1021 cggatagacc ggcgatggtc atgggacaaa aaatattcga actcgtcctg gcttcagcag
     1081 tgattgcgag cgggggagag gcgctggcga agcgcgtgcc gccgaaagcg gtcgccgcgg
     1141 tggtcgccaa cggcgtcagg taccaggtcc ctcacttcgg cgcgctccac ggcaaggcgc
     1201 agaacggcgg ctacgtgcag gcctgggacg tgaagacggg caagctgctc tgggaccgga
     1261 tggtgtatcg cgtccggtac gacgagaatc tcgagaaaga cgtccaggac gatttcatct
     1321 cgcggatctc ggtcaggaaa gggcatctca gggtgacgac cgagggctcc gaggagttcg
     1381 atatggacct gaagtcggga gtggtccgcg cgcttacccc gctgggccgc aacatcgagg
     1441 tcgcggaccc gaagccgtga tctggcggaa ggcggtcgcg ctctggatcg tgctcctcgt
     1501 cttcgcgatc gcgctcgcgg gcgtgcgcga ggcggcgctc gcgcctgcgc tcggtgattt
     1561 ccgcgcgcac caggtcgaga ccgtcgcgtt ctgcgcggtg gtcgcggccg tcgcgttctt
     1621 cggcgtgcgc tggatgcgcg cgacgcggcg ccagggaatc cgcgtcggcc tgctgtggct
     1681 ggcgatgacg ctgctgttcg agctcggcgt gttcggcctc gcgctcgggc acccgtggcc
     1741 cgagctgctc gccgactaca atatcctcga agggcggctg tggccgctgg tgctcgtgac
     1801 cgagctcgtg gcgcccgcga tcttcgcgcg ccgtacggaa aaggagaaaa cgcaatgatg
     1861 gccgtgattt tcgaggtgtg gcccgcggag ggcagggcgc aggactattt ctcgatcgcg
     1921 ggacagctga aggccgacct ggaaaagatc gacggcttcg tctcgatcga gcgcttcgag
     1981 agcctcaccg agaagggcaa gttcctgtcg ctctcgttct ggcgcgacga ggacgcgatc
     2041 cggcgctggc gcaacctgga ggggcatcgc gccgcgcagg cgaaggggcg cggggggatc
     2101 ttcgcggact accggctgcg cgtggcgtcg gtggtgcgcg actacggcat gggcgagcgc
     2161 gcgcaggcgc cgaaggacag ccgcgcggtg cacgacacgc gctgaagcgt cacggccccg
     2221 tcgcgggcgt cacgccgaac acgtccggcg ccttcgggtc gatccacgcc ttgatccagc
     2281 gcacgaaggg cgagccccag acttcgacgc gggtgaagct cccgatcgtc ttcccgctcg
     2341 cgtcggcgag cggatggtcg tagacgaagt ggtgcctgtc gccgtgcacg tagagcagcg
     2401 gcttcccgag cttccgcagg tacagcgcgt gcgcgcgcag gacgttgcgg aagctctccc
     2461 agccgtcggg cacgccgggc ctgcgcctca ccctcttctc gaaatcggga tccgcctggg
     2521 cgaagacgac cagcccgcgc aggtcgcgct gctcgacgag cttcaccgcc tcgtccagcc
     2581 agtcgaagac cgcggccatg cgctgctccg cctcgcggtc catcgcgggg ttgcgcccga
     2641 cgttgttgtt gctgccctgg acgttgacgc cgacgaacag cacgtcgccc gcggtccagc
     2701 gcatgtcctc gcgatactcg gggaagcggc ggtcgctcga ctggcgctcg agcctgatct
     2761 tctgttgtcc caggctctcg tcgccagact cgaacagctt cctgaaaaaa tcgagccgct
     2821 cgagcgggtc cttgccgctg cggtggcagt cgacccagtc gttgtcgccc gggacgatga
     2881 cgaaggggtg cttcgagcgc tggaactggc gcttgcgcgc ctcgaaccat tcgtcgttgc
     2941 acggaccgtg acccgcggtg atgtccccga cgtggatcac gaacgccaag tcctgcgcgt
     3001 tcagggcgtc gatgaggcgg tcgacctcgt cgacctcgag cgtgttgtag ggcgcgtcgc
     3061 cgatcagcgc gaacgcgatc gacccgggac ccggctgcgc gaaggtgccg cagccggcga
     3121 ggagcaggac gagggcgcag gcggcgagcg cagggcggcg catggcggcg cagtctacgc
     3181 ccgctttatg acggtttccg cagcttcgcc gtcgcggccg tgtcgaccgc gagatttccg
     3241 tccagcgcga cgccgtagag ctcgcgcgcc gcgcgcggcg acacgtagcc ctgccgcacg
     3301 tcctcggcga cccgctccgg atcgcgctcg aacggcgctc cgaacccgcc gccgccgccc
     3361 gcgcgcaggg tgaaggcgtc gcctttctgg agcctttgcg tcagcacctt gccgttcggc
     3421 aggtcggtct tctcgacgcc ccccaggcgc agcgtcacct ggttgcccgc gcccgcgagc
     3481 ccgccctcga ggccccacgg cgggcagtgc atgcggtcga cctgcacgtt catcacgagg
     3541 gtcgagcgcg cgcgcacgac ctgctcggtg ccgaggccgc cgcggtggcg tcccgctccg
     3601 cccgagtcct cgcgcagcgc gtgccgctcg aacagcagcg ggaacttcgc ctccatctgc
     3661 tcgatggggt ggttgtgcgt gtcgccgtcg ttcatgcaga ccgtggcgct catcccgtcc
     3721 tcgtcgtgct tcgcgcccca gccgccgccc gagggcccga cgccgccgag ga
//
LOCUS       DATDMW010000135        13154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_538103, whole genome shotgun sequence.
ACCESSION   DATDMW010000135 DATDMW010000000
VERSION     DATDMW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13154
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538103"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VI319_05780"
     CDS             complement(<1..257)
                     /locus_tag="VI319_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY6863390.1"
                     /translation="MNADLHCHSTASDGLLAPAALIERACARGVTMFALTDHDAIAGL
                     DEARGAAQARGLRFIDGVEISVTWRETTIHVLGLNIDPANE"
     gene            complement(268..1140)
                     /locus_tag="VI319_05785"
     CDS             complement(268..1140)
                     /locus_tag="VI319_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6863391.1"
                     /translation="MKPSESLFVDVRGLRYHCRCWGRAGQPALFLLHGWMDVSASFQF
                     LVDAFARDWRVIAPDWRGFGLSERAKDGAYWFPDYFADLDALLAHFHAAAPATLIGHS
                     MGGNVAAMYAGIRPERVARLVNLEGFGLAATDPAQAPKRYARWLAELAGDPPSFRDYD
                     SFDALAARLREGNPRLTSERAAFLARHWGRPRPDGRVEIASDPAHKLVNPVLYRVEEA
                     EACWRNVVAPVLWVAGAETKIPSALKLEPHSLAARKACFRDLAERVVPDAGHMLHHDQ
                     PEKLAAVIEEFLAC"
     gene            1189..1716
                     /locus_tag="VI319_05790"
     CDS             1189..1716
                     /locus_tag="VI319_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY6863392.1"
                     /translation="MSIFSLGERKVVVRGDEWFVAPNATVVGSVVIENQASVWFNVVV
                     RGDNDLITIGERSNVQDGSVLHTDPGIKLTLGRGVSIGHKAMLHGCTVHDGALVGINS
                     VILNHAVIGKGSLVGANSLVPEGKVIPEGVLALGTPAKVLRELKPEERALLASIADGY
                     VERARIFRAQMKPQD"
     gene            1719..2021
                     /locus_tag="VI319_05795"
     CDS             1719..2021
                     /locus_tag="VI319_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863393.1"
                     /translation="MRHKALIAVVVVAALAILLAVSNPGPEVHKQAIRDAVYKDQPLA
                     SALGLGVLSSELATYESYLFWSTMTLQDERVSFGMLGRVRVDVDRLKLPKADPSAR"
     gene            complement(2053..2820)
                     /locus_tag="VI319_05800"
     CDS             complement(2053..2820)
                     /locus_tag="VI319_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY6863394.1"
                     /translation="MKISGSTFLVTGGASGLGEGTVRMLAESGANVVVADLQVDKGEA
                     IAKQLGAKARFVKCDVTSEADGQAAVERALRDFGALQGLVNCAGIAIGERTVKKDGPH
                     ALASFTRVIQINLIGTFNMIRLAATAMTKGQPNAAGERGVIVNTASVAAFDGQIGQAA
                     YSASKGGVVGMTLPIARDLSRDGIRVCTIAPGIFETPMLLGMPKEVQEALGKQVPFPS
                     RLGKPAEYAALVKHIVENEMLNAEVIRLDGAIRMAPK"
     gene            complement(2835..3992)
                     /locus_tag="VI319_05805"
     CDS             complement(2835..3992)
                     /locus_tag="VI319_05805"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEY6863395.1"
                     /translation="MSRKQFLVVLAVLLGLAVAAVAVVLSDNGGWRGGEAQAGRSITP
                     DLKVSDVAEIGVVGASGAVHLVRSDRGWVVRERGDFPADLERIRDILIKLAEVRVAQA
                     EPLPQSQRARLQLVEPADKNADKDAKDAGTVLELKDAKGKVLAHLLLGKKVYRQTEVA
                     GPAGAPPEATGRYLIAGTDADTMLVVPDPFNAAEPRPESWLRKELLRAERVKTVTSIG
                     SDRRTRWTLTRDKDNMDWRFAGSREQPDLQKATDAAGAFGWMDVVDVVVDPQSAETGL
                     SHAVTVRAETFDNVTYTVRIGNKTGDNYYVAVSVAGEPPQSRTPESGETAEEKQKKDK
                     NFADDKARLAERLERERGLDRWRYVVGKNMIEPLLRDRAQMLPEKKTGKKT"
     gene            complement(3989..5848)
                     /locus_tag="VI319_05810"
     CDS             complement(3989..5848)
                     /locus_tag="VI319_05810"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY6863396.1"
                     /translation="MKKYEAFLYSAGGLAAIFVVLLLVNFVLGALPVRIDLTQGKLYT
                     LSDGTREILRKLDAPVKLRLYFSQSDASVPLAIKAYGRHVEDLLAEYRRASGGKVQVE
                     KLDPQPDSDAEDAATLEGVEAQVAPTGDKFYLGLSVSYVDQKVAIPALSPERERLLEY
                     DLTRAIARATSPGKPVVGVMSPLPVFGAPPSPMTGGQPMEQQVFVNELQRDYTVKRVS
                     PAAEKIDDDVKVLVVIHPRGIGDAAQYAIDQFVLRGGKLVAFVDPAAYFDPMAQMSGG
                     PTSSSLDKLFKAWGIQYNPGKVVMDMRYITGSGPRALPTLLSLNDNAFDPNDISTSRL
                     GSALLPFSGVLAGKPADGLKETVLMKTSPFSQLVDASSAISQGEAAIRGFQPSNTEYP
                     LAIRLTGKFRTAFPDGKPKPEDKKAPPKAPDAKPEAPQLKDSKGDTAVALVADSDFVN
                     DGAAVQVQELFGQRIVIQRNGNLAFAQALVDQFAGDPALVELRSRASASRPFTVIRDM
                     EARAQQAYLGKIKALEDSLNQTQEKLAQLQRARAPGASTILSPEQQAELENFRKKAAE
                     TRRELKDVRKDLRADSEALETWTKVVNIAAMPLLVAVAGLGLALWRRRRKVTL"
     gene            complement(5850..6590)
                     /locus_tag="VI319_05815"
     CDS             complement(5850..6590)
                     /locus_tag="VI319_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6863397.1"
                     /translation="MSRSIVATITRRELGAYFQSPVAYVFLVIFLLLAGVFTFTFGGF
                     FERGEASLSALFNWMPWLFLFLVPAVGMRLWSEERRLGTMELLLTMPVASWQAILGKF
                     VASWLFLAIALALTFPMIITVNWLGDPDNGVIASGYVGALLLAGAYLAISSMTSAMTR
                     NQVIAFIVAVVLCLLLILVGFSPVTDMLSRWASPRVVESVARFSVLTHYDGFQRGVIE
                     SRDLAYFLSVIAFSLFATGVVIRSHRAG"
     gene            complement(6587..7564)
                     /locus_tag="VI319_05820"
     CDS             complement(6587..7564)
                     /locus_tag="VI319_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6863398.1"
                     /translation="MIKVDNLSKSFGAKLAVDGVSFEVGRGEVLGFLGPNGAGKSTTM
                     RMITGFIPPSGGSVSVCGHDMVADPIPGKRRIGYLPEAAPSYTDMTVLSFLEFAADIR
                     GLSGDARRKAVRRAVDACYLEPVLGQTIDTLSKGFRHRTCFAQAIIHDPDVLIMDEPT
                     DGLDPNQKHEVRTLIREMGQNKAIIFSTHILEEVEAACTRAIIIDRGRIVANGTPDEL
                     KARSDRAGAVLVKLGGASAQAVSAALADSPLVARSEILATDPKWVVARAYPRKPAPEG
                     ALAARVAELAVRERWKIEELVVEEGRLDDVFRSLTWSETASKAREEAKA"
     gene            complement(7693..8409)
                     /locus_tag="VI319_05825"
     CDS             complement(7693..8409)
                     /locus_tag="VI319_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863399.1"
                     /translation="MSAPAALELRGVHAYYGRAHILADVGFTAARGEVTVLVGRNGAG
                     KSTTLKTIMGLVRAARGEILFEGRRIERREAYEIARLGVGYVPEDRRIFAGLSVAENL
                     EVGRQPPREGAPHWTPEALFALFPNLASVRDRRGDRISGGEQQMLAIARALMGNPRCL
                     LLDEPSEGLAPLIVEGLAAAILELKAQGVAVLLSEQNVRFAERVGDRACVIEQGRVRF
                     SGTLDELARDEAARAATLGV"
     gene            complement(8406..9149)
                     /locus_tag="VI319_05830"
     CDS             complement(8406..9149)
                     /locus_tag="VI319_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863400.1"
                     /translation="MTALAVSGLAKSFGAVRAVDGVDFEVGSGELVAMIGPNGAGKST
                     CFNMLSGQLAPDAGTIRFDGTEIQGRRPRDIWRLGVGRTFQVAAVFASMSVRENVQVA
                     LLSRHRRLAGFWRRAAAQHVGEAGALLDRVGMLGQADRPCAVLAYGDLKRTELAIALA
                     NQPRLLLMDEPTAGMAPRERNALMGLAASIVRERGIAVLFTEHDMDVVFRHADRILVL
                     DRGRIVARGAPAEVRADRQVRRIYLGSAP"
     gene            complement(9146..11023)
                     /locus_tag="VI319_05835"
     CDS             complement(9146..11023)
                     /locus_tag="VI319_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6863401.1"
                     /translation="MASFVAQFLNGLASASTLFLIASGLSLIFGVTRVVNFAHGSLYM
                     LGLYVAYSAAGRVGFWPAIAVAATVVAAAGALIELAVLRRIYRQPAMFQLLATFAVAL
                     IVKDACLWIWGPEDLLGPRAPGLRDAVDVLGATVPSYDLFLVAVGPAVLGLLWLALRR
                     TRWGMLVRAATEDRDTASALGVDSKRLFTGVFALGALLAGLGGALQLPREPAQLALDF
                     AAVSDAFVVVVVGGMGSIPGAFLAALIIGEVKALCIALGTVHVLGVSVSFSKLTLVAE
                     FLVMAVVLVLRPWGLLGSSQPDAQRIGISLEPAPSAASLPARHAGAIVLAALAAFPFV
                     APEYGVVLMNDVLVFALFAASLHFIMGPGGLVSFGHAAYFGLGAYGAGLLVKHAGWGM
                     EASLGGGLLAAALGAALFGWFCVRLSGVYLAMLTLAFAQIAWSIMFQWDEVTGGSNGI
                     VGLWPPDWLASRTAYYLATLALAAAAVALMRRALSAPFGWALRASRDSPLRCGAIGID
                     VARVQWIAFIVAGAAAGLAGALYAFSKGSISPESTMAIGRSVDALVMVLLGGLHSPWG
                     PLAGAAAFTWLQDTVARSSDYWRALLGGVILLLVLVFPRGIAGSLPRRSRRERDRRDE
                     A"
     gene            complement(11028..12221)
                     /locus_tag="VI319_05840"
     CDS             complement(11028..12221)
                     /locus_tag="VI319_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6863402.1"
                     /translation="MRSILRLLLAAAILSAGPAAAQDAVKIGELNSYKVFPAFLEPYR
                     KGMELALDEINAAGGVHGRKLELVIRDDNGNPGDAVRVAEELLAREKVDLLMGTFASN
                     VGLAVADLARQRKVLFLASEPLTDKLVWQSGNRYTFRLRPSTWMQTAMLVDEAAKLGK
                     KRWAIVYPNYEYGQSATAAFKELLKARQPGVEFVAEQAPPLGKIEAGAVVQALADAKP
                     DAIFSSLFAGDLAKFVREGNQRGAFRNRTVLNLLGGEPEYLDPLKDETPTGWYVTGYP
                     WSQIRTPEHQRFLDAYRKKYDDYPRLGSVVGYSAVKSAAAAVAKAGGTDTEKLVEAMK
                     DLRVDTPFGRITYRAIDHQSTMGAFVGRTALKDGKGVMTSFRYVDGAKVQPSDAEVRK
                     LRPAE"
     gene            12386..12709
                     /gene="fdxA"
                     /locus_tag="VI319_05845"
     CDS             12386..12709
                     /gene="fdxA"
                     /locus_tag="VI319_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY6863403.1"
                     /translation="MTHVVTESCIKCKYTDCVDVCPVDCFREGPNMLAIDPDECIDCA
                     VCIPECPVNAIYAEEDLPEDQKGFTALNAELAKIWPAITDAKEAPEDADQWKDVKDKQ
                     QHLER"
     gene            12726..>13154
                     /locus_tag="VI319_05850"
     CDS             12726..>13154
                     /locus_tag="VI319_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863404.1"
                     /translation="MLRLGHGLEFPDLYRREGLLRLDTAFLETLAAADAGLKDRLVAA
                     RRDPSALPAKEESELILEVAPHLDDFLASLFGIAPEFAALSARHHELAPLYSVKRQFV
                     QRRAANKIKPEQAEALDGPALEARLTPLLGSFDELAFARKV"
BASE COUNT         2011 a         4650 c         4587 g         1906 t
ORIGIN      
        1 tcttcgttcg cgggatcgat gttcagcccg aggacgtgga tcgtggtctc gcgccaggtc
       61 accgagatct cgacgccgtc gatgaagcgc agcccgcgcg cctgcgccgc gccgcgcgcc
      121 tcgtcgagcc ccgcgatcgc atcgtgatcg gtcagcgcga acatggtcac gccgcgcgca
      181 cacgcgcgct cgatcagcgc cgcgggcgcg agcagcccgt ccgacgccgt cgaatggcaa
      241 tgcaggtccg cgttcatgcg aaaaatctta gcacgcgaga aattcctcga tcaccgcggc
      301 gagcttctcc ggctggtcgt ggtggagcat gtgtcccgcg tcgggaacga cgcgctcggc
      361 caggtcgcga aagcacgcct tgcgcgcggc gagcgagtgc ggctcgagct tgagcgccga
      421 ggggatcttc gtctcggcgc ccgcgaccca gaggacgggc gcgacgacgt tgcgccagca
      481 ggcctcggct tcctcgacgc ggtagagcac cgggttcacg agcttgtgcg cgggatcgct
      541 cgcgatctcg acacgcccgt cgggcctcgg ccggccccag tggcgcgcga ggaacgcggc
      601 gcgctccgag gtcaggcgcg gattgccttc gcgcaggcgc gcggcgagcg cgtcgaagga
      661 gtcgtagtcg cggaacgacg gcggatcgcc cgcgagctcg gcgagccagc gcgcgtagcg
      721 cttgggagcc tgcgccgggt cggtcgccgc gaggccgaag ccctcgaggt tgaccaggcg
      781 cgcgacgcgc tcgggccgga tgccggcgta catggcggcg acgtttccgc ccatgctgtg
      841 cccgatcagc gtcgccggcg cggcggcgtg gaagtgcgcg agcagcgcgt cgagatcggc
      901 gaaataatcg gggaaccagt aggcgccgtc cttcgcgcgc tcgctcagcc cgaatccgcg
      961 ccagtcgggc gcgatcacgc gccagtcgcg cgcgaaggcg tcgaccagga actggaacga
     1021 agccgagacg tccatccatc cgtgcaggag aaagagcgcc ggctgccccg cgcggcccca
     1081 gcagcggcaa tggtaacgga ggccgcggac gtcgacgaac agggattcgc tgggcttcat
     1141 ctcgggtgct taggataaca ggcgcttctg ctatcatcgg ccgcgatcat gtcgatattc
     1201 tccctcggcg agcgcaaggt cgtcgtgcgc ggcgacgagt ggttcgtggc gccgaacgcg
     1261 accgtcgtcg gctcggtcgt catcgagaac caggcgagcg tgtggttcaa cgtcgtcgtg
     1321 cggggcgaca acgacctcat cacgatcggc gagcgcagca atgtccagga cggctcggtg
     1381 ctgcacacgg accccggcat caagctcacc ctgggccggg gcgtctcgat cggccacaag
     1441 gcgatgctgc acggctgcac cgtgcacgat ggcgcgctgg tcggcatcaa cagcgtgatc
     1501 ctgaaccacg ccgtgatcgg caagggatcc ctcgtcggcg cgaacagcct ggtgcccgaa
     1561 ggcaaggtca tccccgaggg cgtgctggcg ctcggcacgc cggccaaggt gctgcgcgag
     1621 ctgaagcccg aggagcgcgc gctgctcgca tccatcgccg acggctacgt cgagcgggcg
     1681 cggatcttcc gcgcgcagat gaagccgcag gactagccgt gcgccacaag gcgctcatcg
     1741 ccgtcgtcgt cgtcgcggcg ctcgcgatcc tgctcgcggt ctccaacccc ggtcccgagg
     1801 tgcacaagca ggcgatccgc gacgccgtct acaaggacca gccgctcgcg agcgcgctcg
     1861 gcctgggcgt cctgtcctcg gagctcgcga cctacgagtc ctacctcttc tggtcgacga
     1921 tgacgctgca ggacgagcgc gtcagcttcg gcatgctcgg acgcgtgcgg gtggacgtgg
     1981 acaggctgaa gcttccaaaa gcagatcctt cggcgcgcta gcgcgcctca cgatggccgc
     2041 gcattcgcgc ggtcacttgg gcgccatgcg gatggcgccg tcgaggcgga tcacctcggc
     2101 gttcagcatc tcgttctcga cgatgtgctt cacgagcgcc gcgtactccg cgggcttgcc
     2161 gaggcgcgac gggaaaggga cctgcttccc gagcgcctcc tgcacctcct tcggcatccc
     2221 gagcagcatc ggcgtctcga agatcccggg cgcgatcgtg cagacccgga tgccgtcgcg
     2281 cgagaggtcg cgcgcgatcg gcagcgtcat gcccacgacg ccgcccttcg acgccgaata
     2341 ggccgcctgt ccgatctggc cgtcgaaggc tgcgaccgag gcggtgttga cgatgacgcc
     2401 gcgctcgccc gcggcgttcg gctggccctt ggtcatcgcg gtcgcggcga gccggatcat
     2461 gttgaaggtg ccgatgaggt tgatctggat gacgcgggtg aacgacgcga gcgcgtgcgg
     2521 cccgtccttc ttcaccgtgc gctcgccgat cgcgatgccg gcgcagttga cgaggccctg
     2581 gagcgcgccg aagtcgcgca gcgcgcgctc gaccgcggcc tggccgtcgg cctcgctggt
     2641 gacgtcgcac ttgacgaagc gcgccttcgc gccgagctgc ttcgcgatcg cctcgccttt
     2701 gtcgacctgg aggtcggcga cgacgacgtt ggcgcccgac tcggccagca tccggaccgt
     2761 cccttcgccc agccccgacg cgccgccggt aaccaggaac gtgctcccgc tgatcttcac
     2821 gattcggcct ttcgtcaggt tttcttgccg gtcttcttct ccggcagcat ctgcgcgcgg
     2881 tcgcggagca gcggctcgat catgttcttg cccaccacgt agcgccagcg gtcgagcccg
     2941 cgctcgcgct cgagccgctc ggcgagccgc gccttgtcgt cggcgaagtt cttgtccttc
     3001 ttctgcttct cctcggcggt ctcgccgctt tccggcgtgc gcgactgcgg cggctcgccc
     3061 gccaccgaga cggcgacgta gtagttgtcg ccggtcttgt tgccgatccg gaccgtatac
     3121 gtgacattgt cgaaggtctc ggcgcgcacc gtgaccgcgt gcgagagccc ggtctcggcg
     3181 ctctgcgggt cgacgaccac gtcgacgacg tccatccagc cgaacgcgcc ggcggcgtcg
     3241 gtcgccttct gcaggtcggg ctgctcgcgc gagccggcga agcgccagtc catgttgtcc
     3301 ttgtcgcgcg tcagcgtcca gcgcgtgcgg cggtcgcttc cgatcgaggt cacggtcttc
     3361 acgcgctcgg cccggagcag ctctttacgc agccacgact cggggcgcgg ctcggccgca
     3421 ttgaatggat cgggcaccac cagcatcgtg tccgcgtcgg tgccggcaat gagatagcgc
     3481 ccggtcgcct cgggtggggc gccggcgggc cccgcgacct cggtctgccg gtagaccttc
     3541 ttgccgagga gcagatgcgc aaggaccttg cccttcgcgt ccttgagctc gagcacggtg
     3601 ccggcgtcct tcgcgtcctt gtccgcattt ttgtccgcgg gctcgaccag ctgcagacgc
     3661 gcgcgctgcg attggggcag cggctcggcc tgcgccacgc gcacttcggc cagcttgatc
     3721 aggatgtcgc gaatgcgctc gagatccgcc ggaaagtccc cgcgctcgcg cacgacccag
     3781 ccgcgatcgc tgcggacgag gtgcaccgcg cccgaggcac cgacgacgcc gatctcggcc
     3841 acgtcgctca ccttgaggtc gggcgtgatg ctgcggcccg cctgagcctc gccgccgcgc
     3901 cagccgccgt tgtccgagag cacgaccgcc accgccgcga ccgcaaggcc gagcagcacc
     3961 gcgagcacga ccaggaactg cttgcggctc acagggtcac cttgcggcgc cggcgccaca
     4021 gcgcaagccc caggcccgcg accgcgacga ggagcggcat ggcggcgatg ttgacgacct
     4081 tggtccaggt ctcgagcgcc tcgctgtcgg cgcgcaggtc cttgcgcacg tccttcagct
     4141 cgcggcgcgt ctcggccgcc ttcttgcgga agttctcgag ctcggcctgc tgctcgggcg
     4201 acaggatggt gctcgccccg ggcgcgcgcg cgcgctgcag ctgtgcgagc ttttcctgcg
     4261 tctggttcag gctgtcctcg agcgccttga tcttgccgag gtaggcctgc tgcgcgcgcg
     4321 cctccatgtc gcggatgaca gtgaacggcc gcgacgcgct cgcgcggctg cgcagctcga
     4381 ccagcgcggg gtcgcccgcg aactggtcga cgagcgcctg cgcgaaggcg aggttgccgt
     4441 tgcgctggat cacgatgcgc tggccgaaaa gctcctgcac ctggaccgcg gcgccgtcgt
     4501 tgacgaagtc gctgtcggcg acgagcgcga ccgcggtgtc gcctttcgag tccttgagct
     4561 gcggcgcttc gggcttcgcg tcaggggcct tcggcggcgc cttcttgtcc tcgggcttcg
     4621 gtttgccgtc ggggaacgcg gtcctgaact ttccggtcag gcggatcgcg agcgggtact
     4681 cggtgttcga cggctggaag ccgcggatcg ccgcctcgcc ctgcgagatc gccgacgacg
     4741 cgtcgacgag ctgcgagaac ggcgaggtct tcatcagcac cgtctccttc aggccgtcgg
     4801 ccggcttgcc cgcgagcacg ccggagaacg gcaacagcgc cgagcccagg cgcgacgtgc
     4861 tgatgtcgtt cgggtcgaag gcgttgtcgt tcagcgagag cagcgtcggc agggcgcgcg
     4921 gccccgagcc ggtgatgtag cgcatgtcca tcaccacctt gcccgggttg tactggatcc
     4981 cccaggcctt gaacagcttg tcgaggctgg acgaggtcgg cccgcccgac atctgcgcca
     5041 tcggatcgaa atacgcggcc gggtcgacga aggcgacgag cttgccgccg cgcaggacga
     5101 actggtcgat cgcgtactgc gccgcgtcgc cgatgccgcg cggatggatc accaccagga
     5161 ccttcacgtc gtcgtcgatc ttctcggcgg cggggctcac gcgcttgacc gtgtagtcgc
     5221 gctggagctc gttgacgaag acctgctgct ccatcggctg cccgcccgtc atcgggctgg
     5281 gcggcgcccc gaacacgggc agcgggctca tcacgccgac cacgggcttc ccgggcgacg
     5341 tcgcgcgcgc gatcgcgcgc gtgaggtcgt actcgagcag ccgctcgcgc tcgggcgaga
     5401 gcgccgggat cgcgaccttc tggtcgacgt agctcaccga cagcccgagg tagaacttgt
     5461 cgccggtggg cgcgacttgc gcctcgacgc cctcgagggt cgccgcatcc tcggcgtcgg
     5521 agtcgggctg cgggtcgagc ttctcgacct gcaccttgcc gccggaggcg cgccggtatt
     5581 cggcgagcag gtcctcgacg tggcggccgt aggccttgat cgcgagcggc acgctcgcgt
     5641 cgctctggct gaagtacagc cgcagcttca ccggggcgtc gagcttgcgc aggatctcgc
     5701 gcgtgccgtc cgagagcgta tacagcttcc cctgggtgag gtcgatgcgc acgggaagcg
     5761 cgccgaggac gaaattgacg agcagcagca cgacgaagat cgcggccagc ccgccggcgg
     5821 aatagaggaa ggcctcgtat ttcttcatgt cagccggcgc ggtggctgcg gatcacgacg
     5881 cccgtcgcga acagcgagaa cgcgatcacc gagaggaagt acgcgaggtc gcgtgactcg
     5941 atgacgccgc gctggaaccc gtcgtagtgc gtgagcaccg agaagcgcgc gaccgactcg
     6001 accacgcgcg ggctcgccca gcgcgagagc atgtcggtca ccggcgagaa gccgaccagg
     6061 atcagcagca ggcacagcac caccgcgacg atgaacgcga tcacctggtt gcgcgtcatt
     6121 gccgaggtca tgctgctgat cgcgaggtag gcgccggcga gcagcagcgc gccgacgtag
     6181 ccgctcgcga tcacgccgtt gtccgggtcg ccgagccagt tgaccgtgat gatcatcggg
     6241 aacgtgagcg cgagcgcgat cgcgaggaac agccacgacg cgacgaactt cccgaggatc
     6301 gcctgccagc tcgccaccgg catggtgagc aggagctcca tcgtgccgag ccgccgctct
     6361 tccgaccaca gccgcatgcc gaccgccggg accaggaaca ggaacagcca cggcatccag
     6421 ttgaagagcg cggacagcga cgcctcgccg cgctcgaaga atccgccgaa ggtgaacgtg
     6481 aagacgcctg cgagcagcag gaagatgacg aggaagacgt acgcgaccgg cgactggaaa
     6541 tacgcgccga gctcgcgccg ggtgatggtg gcgacgatcg agcggctcat gccttcgcct
     6601 cctcgcgcgc tttcgacgcg gtctcggacc aggtcagcga ccggaacacg tcgtcgagac
     6661 ggccctcctc gaccaccagc tcctcgatct tccagcgctc gcgcaccgcc agctcggcga
     6721 cgcgcgcggc gagcgcgccc tcgggcgcgg gcttgcgcgg atacgcgcgc gcgaccaccc
     6781 acttgggatc ggtcgcgagg atctcggacc tcgcgacgag cgggctgtcg gccagcgccg
     6841 cgctcaccgc ttgcgcgctc gcgccgccga gcttgaccag caccgcgccg gcgcggtccg
     6901 agcgggcctt cagctcgtcg ggcgtgccgt tggcgacgat gcgcccgcgg tcgatgatga
     6961 tcgcgcgcgt gcacgccgcc tcgacctcct cgaggatgtg cgtcgagaag atgatcgcct
     7021 tgttctgccc catctcgcgg atcagcgtgc gcacctcgtg cttctggttg gggtcgaggc
     7081 cgtcggtggg ctcgtccatg atcaggacgt cgggatcgtg gatgatcgcc tgcgcgaagc
     7141 aggtgcggtg gcggaagccc ttcgacagcg tgtcgatcgt ctgcccgagc acgggctcga
     7201 ggtagcacgc gtcgaccgcg cggcgcaccg ccttgcggcg cgcgtcgccg ctcaggccgc
     7261 ggatgtcggc ggcgaactcc aggaacgaga ggaccgtcat gtccgtatac gacggcgcgg
     7321 cctcgggcag gtagccgatg cgtcgcttcc cggggatcgg gtccgcgacc atgtcgtgac
     7381 cgcagacgct cacggagccg ccgctcggcg ggatgaaccc cgtgatcatg cgcatggtgg
     7441 tcgacttgcc ggcgccgttg gggccgagga acccgagcac ctcgccgcgc ccgacctcga
     7501 aggagacgcc gtcgacggcg agcttcgcgc cgaacgactt gctgagattg tcgaccttga
     7561 tcatgtccgg aatcgccgcc cggcctgaag aaaacatcgc ggcggcaagc tgggtttctc
     7621 ttgtcggaat ttcaagcccg agattatcgc agcttgggcg ccgccgtaaa ggtcttcggg
     7681 aattacgttt ggttacacgc cgagggtcgc cgctctggcc gcctcgtcgc gcgccagctc
     7741 gtccagggtc cccgagaacc gcacgcgccc ctgctcgatc acgcacgcgc ggtcgccgac
     7801 gcgctccgcg aagcgcacgt tctgctccga gagcagcacc gcgacgccct gcgccttcag
     7861 ctcgaggatc gcggccgcga ggccttcgac gatcagcggc gcgaggccct cggagggctc
     7921 gtcgagaagc aggcagcgcg ggttgcccat cagcgcgcgc gcgatcgcga gcatctgctg
     7981 ctcgccgccc gagatgcggt cgccgcgccg gtcgcgcacc gaggccagat tggggaacag
     8041 cgcgaacaag gcctcgggcg tccagtgcgg cgcgccctcg cggggcggct ggcggccgac
     8101 ctcgaggttc tcggcgaccg acagtcccgc gaaaatgcgc cggtcctcgg gcacgtagcc
     8161 cacgcccagg cgcgcgatct cgtaggcctc gcggcgctcg atcctccggc cctcgaagag
     8221 gatctcgccc cgcgcggcgc ggacgagccc catgatcgtc ttcagcgtgg tcgatttccc
     8281 ggcgccgttg cggccgacga gcaccgtcac ctcgccgcgc gccgcggtga agccgacgtc
     8341 ggcgaggatg tgcgcgcgcc cgtagtacgc gtgcacgccg cgcagctcga gggccgccgg
     8401 cgcgctcacg gcgcgctccc gagatagatg cgccgcacct ggcggtccgc gcgcacctcg
     8461 gccggcgcgc cccgcgccac gatgcgcccg cggtcgagca ccaggatgcg gtcggcgtgc
     8521 cggaacacga cgtccatgtc gtgctcggtg aagagcaccg cgatgccgcg ctcgcggacg
     8581 atcgaagccg cgaggcccat gagcgcattg cgctcgcgcg gcgccatgcc ggccgtgggc
     8641 tcgtccatga gcagcaggcg gggctggttg gcgagcgcga tcgcgagctc ggtgcgcttg
     8701 agatcgccgt aggcgagcac ggcgcacgga cggtcggcct ggcccagcat gccgacgcga
     8761 tccagcagcg cgccggcttc cccgacgtgc tgcgcggccg cgcgtcgcca gaagccggcg
     8821 agccggcgat gccgcgagag cagcgcgacc tggacgttct cgcgcacgct catcgacgcg
     8881 aagaccgccg cgacctggaa ggtgcggccg acgcccaggc gccagatgtc gcgcgggcgc
     8941 cggccctgga tctcggtgcc gtcgaagcgg atcgtcccgg cgtcgggcgc gagctggccg
     9001 ctcagcatgt tgaagcaggt gctcttcccc gccccgttcg gaccgatcat cgcgaccagc
     9061 tccccgctcc cgacctcgaa gtcgacgccg tcgacggcgc gcaccgcgcc gaagctcttc
     9121 gcgaggccgg acacggcgag cgcggtcatg cttcgtcccg ccggtcccgt tcacgccggc
     9181 ttcgccgcgg cagcgatccc gcgatgccgc gcgggaagac cagcacgagc agcaggatca
     9241 cgccgccgag cagcgcgcgc cagtagtcgc tgctgcgcgc gacggtgtcc tgcagccagg
     9301 tgaacgcggc ggcgccggcg agcggccccc acggcgaatg caggcctccg agcagcacca
     9361 tcacgagcgc gtcgaccgag cgcccgatcg ccatcgtaga ctcgggcgaa atgctgccct
     9421 tcgagaacgc gtagagcgct cccgcgagcc ccgcggcggc gccggcgacg atgaacgcga
     9481 tccactgcac ccgcgcgaca tcgatgccga tcgcgccgca gcgcagcggc gagtcgcgcg
     9541 acgcgcgcag cgcccagccg aagggcgcgg acagcgcgcg ccgcatcagc gccacggccg
     9601 cggcggcgag cgcgagcgtc gccaggtagt acgcggtgcg cgacgcgagc caatccggcg
     9661 gccagaggcc gacgatgccg ttcgagccgc ccgtgacctc gtcccactgg aacatgatcg
     9721 accaggcgat ctgcgcgaag gcgagcgtca gcatcgcgag atagacgccc gagagcctga
     9781 cgcagaacca gccgaacagc gccgcgccga gcgccgcggc gagcagcccg cccccgagcg
     9841 aggcctccat gccccagccg gcgtgcttca ccagcagccc cgcgccgtac gcgccgaggc
     9901 cgaaatacgc ggcgtggccg aacgacacca gcccgcccgg ccccatgatg aaatgcaggc
     9961 tcgcggcgaa cagcgcgaac acgagcacgt cgttcatcag cacgacgccg tactcgggcg
    10021 cgacgaacgg aaatgcggcg agcgccgcga ggacgatggc gccggcgtgg cgcgccggga
    10081 gcgatgccgc ggacggcgcg ggctcgagcg agatcccgat gcgctgcgcg tccggctgcg
    10141 acgagccgag caggccccac ggccgcagca cgagcacgac cgccatgacg agaaactcgg
    10201 cgaccagcgt cagcttcgag aacgacacgc tcacgccgag cacgtgcacc gtgccgagcg
    10261 cgatgcacag cgccttcacc tcgccgatga tgagcgccgc gaggaacgcg cccgggatgc
    10321 tccccatccc gcccaccacc acgacgacga aggcgtcgga gactgcggcg aaatcgagcg
    10381 cgagctgcgc cggctcgcgc ggcagctgca gcgcaccgcc gaggcccgcg agcagcgcac
    10441 cgagcgcgaa cacgccggtg aacaggcgct tcgagtcgac acccagggcc gacgccgtgt
    10501 cgcggtcctc ggtcgcggcg cgcacgagca tgccccagcg cgtgcgccgg agcgcgagcc
    10561 acagcagccc cagcaccgcg ggacccaccg cgaccaggaa gaggtcgtag ctcggaaccg
    10621 tcgcgccgag aacgtcgacc gcgtcgcgca ggccgggcgc gcgcggcccg agcaggtcct
    10681 cggggcccca aatccacagg cacgcgtcct tgacgatcaa ggccactgcg aaggtcgcga
    10741 ggagctggaa catcgccggc tggcgataga tccggcgcag cacggcgagc tcgatcaggg
    10801 cgccggcggc ggcgacgacc gtcgcggcca ccgcgatcgc gggccagaac ccgacgcgcc
    10861 cggcggcgct gtacgcgacg tagaggccga gcatgtacag cgacccgtgc gcgaagttga
    10921 cgacgcgcgt gacgccgaag atcagcgaca ggcccgacgc gatcaggaac agcgtcgacg
    10981 cgctcgcgag cccgttcagg aactgcgcga cgaagctcgc cacggtccta ttcggcgggg
    11041 cgcagctttc ttacttcggc gtcgctcggc tgcaccttcg cgccgtcgac gtaccggaac
    11101 gacgtcatga cgcccttgcc gtccttcagc gcggtgcgtc cgacgaacgc gcccatggtc
    11161 gactggtggt cgatggcgcg gtaggtgatg cggccgaaag gcgtgtcgac gcggaggtcc
    11221 ttcatcgcct cgaccagctt ctcggtgtcc gtgccgcccg ccttcgccac cgcggccgcg
    11281 gcgctcttca ccgccgagta gccgacgacc gagccgagcc gcgggtagtc gtcgtacttc
    11341 ttccggtacg cgtcgaggaa gcgctggtgc tcgggcgtcc ggatctggct ccagggatag
    11401 ccggtcacgt accagccggt cggcgtctcg tccttgagcg ggtcgagata ttcgggctcg
    11461 cccccgagca ggttgagcac ggtgcggttc ctgaacgcgc cgcgctggtt gccctcgcgc
    11521 acgaacttgg cgaggtcgcc ggcgaacagc gacgagaaga tcgcgtcggg cttcgcgtcg
    11581 gcgagggcct ggacgaccgc gcccgcctcg atcttcccga gcggcggcgc ctgctcggcg
    11641 acgaactcga cgccgggctg ccgcgccttc agcagctcct tgaacgccgc ggtcgccgac
    11701 tgtccgtact cgtagttggg atagacgatc gcccagcgct tcttcccgag cttcgccgcc
    11761 tcgtcgacca gcatggcggt ctgcatccag gtcgaaggcc gcagccggaa cgtgtagcgg
    11821 ttgccgctct gccacacgag cttgtcggtg agcggctcgg acgcgaggaa cagcaccttg
    11881 cgctggcgcg cgaggtcggc gacggcgagc ccgacgttgg acgcgaacgt gcccatcagc
    11941 aggtcgacct tctcgcgcgc gagcagctcc tcggcgacgc gcaccgcgtc gcccgggttg
    12001 ccgttgtcgt cgcggatcac cagctcgagc ttgcgcccgt gcacgcctcc ggccgcgttg
    12061 atctcgtcga gcgcgagctc catgcccttg cggtaaggct cgaggaaggc gggaaagacc
    12121 ttgtagctgt tgagctcgcc gatcttcacc gcgtcctggg ccgcggcggg accggcggag
    12181 aggatcgcgg cggcgaggag caggcgcagg atggatcgca tcgagaaacc ccggaacgaa
    12241 ggagcgctcg aatatacacc acggcgggcc cgggtcgctg ctcgccgcgc cggtcataag
    12301 caaattaccg ggatgcatct tccggcgggt acggtccacg ccgtataatt tcgcctcttg
    12361 cgcaccgcgc gccgccgagg aagagatgac ccacgtcgtc accgaaagct gcatcaaatg
    12421 caagtacacc gactgcgtcg acgtctgtcc ggtggactgc tttcgcgagg gcccgaacat
    12481 gctggcgatc gatcccgacg agtgcatcga ctgcgccgtg tgcattcccg aatgcccggt
    12541 caacgccatc tacgccgagg aggacctgcc cgaggatcag aagggcttca cggcgctgaa
    12601 cgccgagctc gcgaagatct ggccggcgat caccgacgcg aaggaagcgc cggaggacgc
    12661 cgaccagtgg aaagacgtca aggacaagca gcagcacctt gaacgctagg atcgatcccg
    12721 atcgcatgct gaggctcggc cacggccttg agttcccgga tctgtatcgt cgagagggcc
    12781 ttctccggct cgacaccgca ttcctcgaga cgctcgccgc cgccgacgcc ggcctgaagg
    12841 atcggctcgt cgccgcgcgc cgcgatcctt ccgcgcttcc cgccaaggag gagtcggagc
    12901 tgatcctcga ggtcgcgccg catctcgacg acttcctcgc gtcgctcttc ggcatcgcgc
    12961 ccgagttcgc ggcgctctcg gcgcgccacc acgagctcgc cccgctctac agcgtcaagc
    13021 gccagttcgt gcagcggcgc gcggccaaca agatcaagcc cgagcaggcg gaggcgctcg
    13081 atggtccggc gctcgaggca cggctgacgc cgctgctcgg gagcttcgac gagctcgcgt
    13141 tcgcgcgcaa ggtg
//
LOCUS       DATDMW010000136        18807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1285565, whole genome shotgun sequence.
ACCESSION   DATDMW010000136 DATDMW010000000
VERSION     DATDMW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285565"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VI319_05855"
     CDS             complement(<1..506)
                     /locus_tag="VI319_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6863405.1"
                     /translation="MRFVVTLARITVLEALRERLLWLAALVVITGAALAQFLHQVAIT
                     ESREIETAVLAALLRMSAAFIVAIVVVSGMAREWNDKITELLLSFPVHRSRYVFGKLL
                     GYAAVCGLLAVVFAVPLALYARSSGVLAWTASLVCELLIVAAVGVFCTLTLTRTLSAL
                     AAVAGFYLL"
     gene            619..2337
                     /locus_tag="VI319_05860"
     CDS             619..2337
                     /locus_tag="VI319_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863406.1"
                     /translation="MAKTRLLQLSQVVISRSWRLLFVLMLVLLHVCAMRGAEDFWARG
                     LMLAHFGLFIIWQPFMRSEQRLTVPQLLVVAAIAVGTLLYLNWWLLALWVSVLAGIVG
                     GKVFLFQARWLRRFYLVVLLYLVMLLLVWIVPNSFLDVPVEPEAVTLAQYGLPALFLL
                     MILIPAEADSAETPQIVDFFYAALIFLLLVVLVLGSFALMTIADVNYVAALSYSMLII
                     AGVLIVLSLAWNPRAGFSGLAMYFSRYLLSIGLPFEQWLYFLAELSQLETKPERFLKE
                     ACAGLGRLPWVNGGFWHTAGESGDFGTVSKNSVEYINQELKLTVFTKLPLSPSLMWHF
                     HLLGQLLGEFYIAKQREQKLQQQTYVQAVHETGARMTHDVKNLLQSLNVLCAAAERDT
                     SPESGELPSLIRRQLPAITQRLQQTLDKLQKPQAESGRFVAARSWWEALQRSYSSRGV
                     EFAVEDIDETVLLPKELFDSSGDNLLQNALRKRKIDPAVAVRASFQCRDTIQFSVCDT
                     GTPVAAEVLKGLLRGPVPSDTGFGIGLYQTSRLAEISGYSLQLTTNEPGRVCFSLLGE
                     VRRGGR"
     gene            complement(2344..3108)
                     /locus_tag="VI319_05865"
     CDS             complement(2344..3108)
                     /locus_tag="VI319_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY6863407.1"
                     /translation="MNGRHRGFTLPELAIALVIIALLLAGAIIPLSAQIDVRNTTETQ
                     RTMDQIKEAAISFALANGRLPCPADGTVAAGAAGAGAENCAISAGVVPWAALGVPETD
                     AWGRRFSYWVSPFFADAVGTTFNCTPTPAPTQSSFALCSLGTMTVNNRGSTHAAVAIG
                     TALPVVIVSHGKNGRGSYTPAGTVMNAPPAGTDEAANATHTAGATVFYSRTPTPAAAG
                     CSDSAAGSPFCEFDDLVVMISAPVLAARMVGAGKLP"
     gene            complement(3105..5063)
                     /locus_tag="VI319_05870"
     CDS             complement(3105..5063)
                     /locus_tag="VI319_05870"
                     /inference="COORDINATES: protein motif:HMM:NF027516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-activating E1 FCCH domain-containing
                     protein"
                     /protein_id="HEY6863408.1"
                     /translation="MASGVHKRAPRERGFALIVVVSLVVLIASYLLVSALNKSSSQIS
                     NAREQTTMSALLKAKSALIAYAASEQWQLYKNQSPNQPGGLPCPDQDDDGDADCVGAG
                     ITNSISLIGRLPWKTIGTEDLRDSSGERLWYAVSRNFRKLYGTTVINSDTPGQLTVTG
                     TASGSNIVAVIIAPGQVTQGQNRVTGHNSAAAYLEGFNANDSVNFIFTTNALPTDALN
                     DRLVAITQADLMAAVEPVVSARIERDVKPYLVAYFNQWGAFPFPAKFAEPAPPGPGTN
                     GPATTRPQAAASGPDYSGDASQAAGLLPISTLANTPTPYPWSGSSVTKSSGVARVDIN
                     SCVSVALSSPFPPPSTGWQCSLRVRSDNFFGSDIIFFPRVSLSSSVGTKAGTSLATLP
                     DVSTITTTIDGASATVSSPSIHGSLTAAGAGTVTYDATLPISCMSSCSDRTVVVTIPD
                     LTVSRLTSAADSTITAASNASPIVVTTSAPHNFPTGAAVTISNVSGNGAANGTWTVTV
                     IDATRFSLNGSSGSGTYTSGGTASAAAWFIANEWFRQTYYAVSPGYLPGGSGACTARP
                     TPPAAAIAPSCLMISNLDPAKYPTVNDKRAILILMGRSLTGNTRPSASLAEYFEGANA
                     PLVAAPYAYENRPGVPTSINDRVVVISP"
     gene            complement(5072..5488)
                     /locus_tag="VI319_05875"
     CDS             complement(5072..5488)
                     /locus_tag="VI319_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863409.1"
                     /translation="MTRARALATVVLACCMAGAAAPVAAQEIGRLFFTPQQRQELDRR
                     REANVQESASAAVESSMTVNGHVSRSSGRTTTWINGVPQYDSVRTPDPSRVTIGGHEG
                     DPPVDLKIGQTLDKTRGEVRDELGGGRIVVKRNPTP"
     gene            complement(5485..6099)
                     /locus_tag="VI319_05880"
     CDS             complement(5485..6099)
                     /locus_tag="VI319_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863410.1"
                     /translation="MNLRREDMKRLRLPAAIAAVLLGAGLAFVVASNQYLGQATDKLE
                     NARGQRSAAQERVSKVSEEEREIRESLVFYQRMQRQGMIGPENRLDWIDAIARIKSER
                     KLFEIKYSIEPQKRLDYPGIVPIKGGDFVVSRVKLDMLLLHEEDLLNFLSDLRATGRF
                     YVSVRHCGINRLERGAVSPGQPLMPRLRSDCQIDLITVDQETAS"
     gene            complement(6096..7646)
                     /locus_tag="VI319_05885"
     CDS             complement(6096..7646)
                     /locus_tag="VI319_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863411.1"
                     /translation="MLPDKRLLYLTSHELVAYLWKRGELVEEATFKPGDEGAAAFSEY
                     VSRASDSLFHIVADLVEEDFFQENIPYLRGGDRRALLVRKLAQRYRDTSLAMALSLGT
                     EMTGRREERILYASFSNTQQFQPWLGSLRAIQARIAGVYSIAMLAPLLCRGLGVKSRQ
                     YMIVSLQEAGLRQTYVDNGKARFSRLSKVDRDDPNALADACAAESARFHQYLLNSRIL
                     SRDSTTLDVVVLVPGESRTLYEGACVSNAQLRYRLVDLNFARRSVGLKSAPSRALAEG
                     LYLHVAARARFLAQFADDALRRFYDLWRTQVALILSGAAVFALCLMVSGMKVIQADYV
                     DRAVQSERIAESTASEQYARMQANFPKTPVPAEVLKLTVRNFQTLMRQDAHVRDILAE
                     ISRALASAPQIEVERIEWDIAPLKQPGARDAAPAAQAPGGGEPRVQLVEIGGRLLVQQ
                     ASDYRNISMVVNQFVDALRSRPGVEIVSTKLPFDLAAEKSISGDIGAARGFEIPRFSV
                     VISRRLET"
     gene            complement(7646..8851)
                     /locus_tag="VI319_05890"
     CDS             complement(7646..8851)
                     /locus_tag="VI319_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY6863412.1"
                     /translation="MALFIYKAIDGRGKSSMGQIEAINIVDLEMRLKRMGLDLIRGAP
                     TKPGGGLLRGGGIKRADLINFCFQLEQLSNSGVPLVDALMDLRDSTENLRLREVVSGV
                     VESIQGGASLSQAMTDYPAVFSPVFSSLVRAGETTGRLPEVLKSLAESLKWEDELAAQ
                     TKKLIMYPAFVGSIVIMVTFFLMVYLVPQMVGFIRNMGQEIPWQTRMLIQVSAFFVDY
                     WWALLLAPVVVAVLVKVLAKTNPLVRYQLDRLKLTLPYLGNILRKIILSRFASTFALM
                     YASGITILEAIRSSEETAGNLVIQEGLRIAGQQIADGKNVTTAFQDVGLFPPLVVRML
                     RVGEATGGLDVALNNVSYFYNREVRESIARVQVMIEPALTIFLGAILGWVMLSVLGPI
                     YDVISKIKI"
     gene            complement(8877..10601)
                     /locus_tag="VI319_05895"
     CDS             complement(8877..10601)
                     /locus_tag="VI319_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY6863413.1"
                     /translation="MNSPRMSAQLGPAVKRHIGQILIAQGILTEDQLRIALLEQLKTN
                     QPVGKLLVNLGFVSEATLRDALSEKLGLQSVDLTQIVVDSVALKLVPRDFARRHHIFP
                     VALDRETRKLIIALADTNNIVALDQVRAHLRGEFELETRLAGETEIARAIEQYYGHDF
                     SIDGILHEIETGEIDYQSLQASGDAYSQPVVRLISALLADAVERAASDIHFEPEQNFL
                     RIRYRIDGVLRQIRSLHKTYWPAMAVRIKVMSKMNIAETRAPQDGRISLTLTGRAVDF
                     RVSSQATTHGENIVLRILDRQKGIVPLDGLGLAQEQLALLKKMIARPEGLILVTGPTG
                     SGKTTTLYSVLNHINTDGLNIMTLEDPVEYPMTLIRQTSINEAAKMDFGNGIRSLMRQ
                     DPDVILVGEIRDEDTATMAFRASMTGHQVYSTLHTNSAVGAVPRLLDIGIVPDILAGN
                     IIGIVAQRLVRRLCKHCKQPHEPTPAERALLGSDGERSPKLYRAVGCDQCDYQGYKGR
                     MAIMEILKMDAELDELVAHRATGRELQKTAVAKGFRTLADDGVRRVLDGSTSLEELIR
                     VVDLTDRM"
     gene            complement(10609..11961)
                     /locus_tag="VI319_05900"
     CDS             complement(10609..11961)
                     /locus_tag="VI319_05900"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6863414.1"
                     /translation="MSLLLEALKKAELAKQRAGGTEPARDPEPSPEAAKSVITREKLP
                     DIGEHVEIHSDDLPSAKSPSGEVRESGKPQHLELEIVADPQPAPRPSGLRSERAPADN
                     QDAARRLFEVKEIDYNPRRPFYATLIALGLVAVAYGGYVWWQLQPRYSYNTQATRDAK
                     ERIAAAAAVQRPLPPTTAAAPVSAVPAPGPTPVPVSAPPQQRLASPGVRSATPAVPKP
                     APAPRPFVPTSRAAQTASAPQAGDPVYAPEPGPRQSIAINPASMQVDPELQRAYEAFQ
                     ANDLETARQIYQGILQREPSNHDALLGMAAIDLRMRNYETAQARYLRLLELDPRDGYA
                     QAGLLALNQADPVASESRLKNLLASQPDSPQLLFALGNQYALQSRWGEAQQAYFRALA
                     ADPENPDYAFNLAVSLDHLHQKPQAVEFYRRSLALADRRSATFDRALAAARALDLQQR
                     "
     gene            complement(11958..13544)
                     /locus_tag="VI319_05905"
     CDS             complement(11958..13544)
                     /locus_tag="VI319_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY6863415.1"
                     /translation="MVNNVRVQELLFALARDAKLNVDIHPGIAGTVTLNAIDQTLPQL
                     LARIAKQVDMRWELDGPNLVVMPDSPYLRVYKIDYVNMDRATVATVGVSSQISSGTTT
                     AGAGGPGAAGGGSALNASNTTVRSTATNRFWETLVDNVKDILRETDKIIPAGTVAAPS
                     QSAAAAAASAAGGVAGAAPSSQPPIAVPSATFREAASVIANPEAGVLTIRATSRQHEK
                     IQEFLDQVLVNAKRQVLIEATIAEVQLNNQYQQGIDWSLMRTGPAGFNVAQTSAGSPA
                     GVSTNMFVLGYVASGISSTIRLLESFGTVRVLSSPKLSVINNQTAILKVVDNRVYFTV
                     QANQTATQTVVTTAFTTTPNVVPVGFVMNVTPQISDSDTVLLNVKPSVSRIISFVNDP
                     NPSLASPCGSAPTAAAACQPIVSRIPEVQTREMESMMKVNSGQIAVLGGLIQDSISDT
                     QDRVPLLAEIPLLGGFFTQRNKANVKTELVVFLRPVVVKDPSIDGDYRAFRSSVPDEN
                     FIGRMNPGKQTPLGGPLSPP"
     gene            complement(13854..15299)
                     /locus_tag="VI319_05910"
     CDS             complement(13854..15299)
                     /locus_tag="VI319_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6863416.1"
                     /translation="MYLDHFGLSEPPFRITPNTEFFFQGANRGATLEALVYAICQDEG
                     IVKVSGEVGSGKTMLCRVLMERLPKNVETVFLANPSLSADEILLAIADELGFDMGELR
                     SARLLRALQDRLIALFADGRRVVVLIDEAHAMPKPTLEEIRLLSNLEANRHKLLQIVL
                     FGQPELDDLLDTADMRQLKERITHSFRLDPLRREDVDSYVDFRMRAAGYRGPNVFARP
                     ALELIAQASQGLTRRVNILADKALLAAFAAGSHAVGPREVRRAIGDSNFYRPARRLGR
                     VAAAVGALVTSLALGWMLHALLADPGRAEPAATVRSPASVAPLPAAQAPAPAAAASAS
                     APKPVPVTTEFSNPKVTEDAKLVVTDAGVPARTDTTAVALWRDRLAATQRLLDAAPGE
                     RQAIQLLTVEAEDVQNLENFLLKASKLVPADELLVYSVKFGGKQHYRVAFGNYADARE
                     ALVAANRLPPLLRALGPYPRSFERMRSQNRQ"
     gene            complement(15329..16753)
                     /locus_tag="VI319_05915"
     CDS             complement(15329..16753)
                     /locus_tag="VI319_05915"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6863417.1"
                     /translation="MPDAKPSLLIVDDDPLITDTLSYALGKDFEIMVSDSRQHAVSLL
                     RQLDDAPQLALVDLGLPPMPHRPDEGFQLVTDLLAHSPTMKIIVLSGQNDSANARHAR
                     TLGAAEFVTKPCDAEKLKQVLLGALQFRAAEISKDKAVEADPMIGKSPALQKLKSQVS
                     QYADSPFPALIEGESGTGKDVVANMLHRLSWRRSKPWFALNCAAIAPTLVEPTLFGYT
                     KGAFTGATQNKSGYFEDAGEGTLFLDEIGELPLELQAKLLRVLENGEYQRVGETQQRF
                     SKARVIAATNRDLRQEIRKGNFRADLYHRLSVFTISVPPLREMDDDRGALLEHFRKFY
                     ADQAKVAPFTLDDDAQRTWRDYHFPGNVRELRNIVIRLTTKYAGRKLAPADLEPEFDL
                     EALAEATPGGMPADPGTLVEQAQRHLQRERSFNLDQMLKAWEQGYIEAAMKLTRGNVS
                     QAAKMLGINRTTLYSRMESLQKQQ"
     gene            complement(16746..17480)
                     /locus_tag="VI319_05920"
     CDS             complement(16746..17480)
                     /locus_tag="VI319_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863418.1"
                     /translation="MHQPVVRFQGVGKSYGSITALRGIDLEIGAGEIVGVAGVNGAGK
                     TTLMKCLLDFCDIDAGTIELHGIGHRRPESRARLAYLPENFVPPHFLTGREFLAAMRR
                     LAGGTGDGEEVVLDALQLKRATLDRPVRTCSKGTIQKLGLAACLASARDLYVLDEPMS
                     GLDPRARAGVKQVLLERKSAGRTAFLSAHSLADIEEICDHMAVLHEGRLAYVGPPSEL
                     LRRYSVDSLESAFLRCIEETNGGCHA"
     gene            17626..18432
                     /locus_tag="VI319_05925"
     CDS             17626..18432
                     /locus_tag="VI319_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863419.1"
                     /translation="MPTENLVEIENVTFGYDQRTVLHGINLTVPKGAVVAIMGLSGCG
                     KTTLLRLIAGAIRATRGDVRVAGERLNGLSDTGLFALRRKMGMLFQFGALFTDMSVFD
                     NVAFQVREHTRLPAAIARDLVLMKLESVGLRGAADLMPSELSGGMARRVALARTIALD
                     PSLIMYDEPFAGLDPVSLGAIGQLIRKLNDALGATSIIVTHDVAEALEIVDYVYYMSA
                     GRIEFRGTPAEVRASRHPSLRQFAYGEPDGPVPFHYPAPDYRADLSAGSA"
     gene            18432..>18807
                     /locus_tag="VI319_05930"
     CDS             18432..>18807
                     /locus_tag="VI319_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6863420.1"
                     /translation="MSRLAEMLRALGARVVTQWVWRVGFAGRFFWLTLAGSGTSFRRF
                     SLTIREIYFAGVLSLVIILVSGLFVGMVLGLQGYDTLVRYGSSEALGVLVSLSLVREL
                     GPVVAGLLFASRAGSAITAEIGL"
BASE COUNT         3012 a         6088 c         6401 g         3306 t
ORIGIN      
        1 agcaggtaga agcccgccac agccgcgagc gcggacagcg tgcgcgtaag cgtcagcgtg
       61 caaaacacgc cgaccgcggc gacgatgagc agctcgcaaa cgagcgacgc ggtccaggcg
      121 agcacgccgc tggagcgcgc gtagagcgcc aggggcaccg cgaagacgac ggccagcagg
      181 ccgcagacgg cggcatagcc gagaagtttg ccgaaaacgt aacggctgcg atgcaccgga
      241 aacgacagca gcagctcggt gatcttgtcg ttccattcgc gcgccatccc ggatacgacg
      301 acgatcgcga cgataaacgc cgccgacatg cgaagcaggg cggcgaggac cgctgtctcg
      361 atctcgcgcg attccgtgat cgcgacctgg tgcaggaact gcgcgagcgc agccccggtg
      421 atgacgacga gcgccgccag ccacagcagg cgctcgcgaa gcgcctcgag gaccgtgatg
      481 cgcgccaaag tgacgacgaa acgcatgcgg aaaaaatcgg tcagccggac ggacccggca
      541 gcattacaat gacacaaaac gtgcctaccg aagcgtaagg cttgactacc gacgacgcaa
      601 gcgcgtcggg ccgctcaaat ggcaaaaaca agactcctcc agctgtcgca ggtcgtcatc
      661 agccgctcgt ggcggctgct cttcgtcctg atgctggtgc tgctgcacgt ttgcgccatg
      721 cgcggagccg aggacttctg ggcgcggggc ctgatgctgg cccacttcgg gctgttcatc
      781 atctggcagc cgttcatgcg cagcgagcag cggctgacgg tgccccagct tctcgtcgtc
      841 gccgccatcg ccgtcggcac gctcctctac ctgaactggt ggctgctcgc cctgtgggtg
      901 agcgtgctcg cgggtatcgt cggcggcaag gttttcctgt tccaggcgcg ttggttgcgc
      961 cgcttctacc tggtggtgct gctctacctg gtgatgctgc tgctcgtctg gatcgtcccg
     1021 aacagctttc tcgacgtgcc ggtcgagccc gaagccgtga ccctcgcgca atacgggctg
     1081 ccggcgctgt tcctgctgat gatcctgatc ccggccgaag ccgactcggc cgaaacgccg
     1141 cagatcgtcg acttcttcta cgccgcgctg atcttcctgc tgctcgtggt cctggtgctc
     1201 ggcagcttcg ctctgatgac catcgcggac gtgaactacg tcgccgcgct ctcctacagc
     1261 atgctcatca tcgcgggcgt gctgatcgtg ctctcgctgg cgtggaaccc gcgcgcgggg
     1321 ttctccggac tggcgatgta tttctcgcgc tacctgctgt cgatcggtct gccgttcgag
     1381 caatggctct acttcctggc agagctctcg cagctcgaga ccaagcccga gcgtttcctg
     1441 aaggaagcct gtgcgggact gggccgcctt ccgtgggtca acggcgggtt ctggcacacg
     1501 gcgggggaat ccggggattt cgggacggtc tcgaagaatt cggtggagta catcaaccag
     1561 gagctcaagc tcacggtgtt tacgaagctc ccgctgagcc cctcgctgat gtggcatttc
     1621 cacctcctgg gacagctcct cggagagttc tacatcgcca agcaacgcga gcagaaactg
     1681 cagcagcaga cctacgtcca ggccgtgcac gagaccggcg cgcgaatgac gcacgacgtc
     1741 aagaatctgc tgcagtcgct gaacgtcctg tgcgcggctg ccgagcgcga caccagtcca
     1801 gaatcgggcg agctgccctc cctgattcgc cggcagcttc ccgccatcac ccagaggctg
     1861 cagcaaacgc tcgacaagtt gcagaagcct caggccgaga gcgggcgctt cgtcgccgcg
     1921 cgcagctggt gggaggcgct ccagcgcagc tattcgagcc gaggcgtgga gttcgcagtc
     1981 gaggacatcg acgagaccgt gctgctcccc aaggagctct tcgacagctc gggcgacaac
     2041 ctgctgcaga acgcgctgcg caagcgcaag atcgatccgg cggtggccgt gcgcgcgagc
     2101 ttccagtgcc gggacacgat ccaattctcc gtgtgcgaca caggtacgcc ggtcgcggcc
     2161 gaggtgctca aggggctgct gcgcggacca gtgccctcgg acacgggctt cggcatcggc
     2221 ctgtaccaga ccagccgcct cgcggagatc tcgggctact cgctgcagct gacgaccaac
     2281 gagccgggcc gggtctgctt cagcctgctc ggcgaagtga ggcgcggcgg gcgctagcta
     2341 gcgctatggc agcttcccgg cacccaccat gcgcgcggcg agtacgggag ccgaaatcat
     2401 cactacgaga tcatcgaact cgcaaaaggg cgagccggcg gcagaatcgc tgcagcccgc
     2461 ggcagccgga gtaggagtgc gcgagtagaa caccgtcgcg ccggccgtgt gagtggcatt
     2521 cgccgcctcg tccgtaccgg ccggcggtgc gttcatgacc gtccccgccg gggtgtagct
     2581 cccgcgtccg ttcttgccgt gcgaaacgat gacgacggga agggcggtgc cgatcgcgac
     2641 ggccgcgtgc gtggaacctc ggttgttgac ggtcatggtc ccgagggagc aaagcgcgaa
     2701 tgaggactgc gtgggcgcgg gggtcggcgt gcaattgaag gtcgttccta cggcgtcggc
     2761 gaagaagggc gacacccagt acgaaaaccg tcggccccag gcgtccgtct ccggcacgcc
     2821 gagcgcggcc cacggaacga caccggcgga tattgcgcag ttctcggcgc cggcgccggc
     2881 cgcgccagcg gcaacggtcc cgtccgcggg acagggcaat cgaccgttag cgagcgcgaa
     2941 gctgatcgcc gcttccttga tctggtccat cgtgcgctgc gtctccgtcg tgttgcgcac
     3001 gtcgatctgg gcgctgagcg gaatgattgc gcctgcgagg agaagcgcga tgatgacgag
     3061 cgcgatcgcg agctcgggca atgtaaagcc gcggtgccgg ccgttcatgg cgatatcacc
     3121 acgacccggt cgttgatgga cgtcggaacc ccgggacggt tttcgtaggc gtatggcgcc
     3181 gccacgagcg gcgcgttggc gccttcgaag tattcggcga gcgaagcgga aggtcttgtg
     3241 ttcccggtca gggatcgccc catcaaaatc agtattgcgc gcttgtcgtt gaccgtcggg
     3301 tatttggccg gatcgagatt gctgatcatc aggcaagacg gtgcgatcgc cgctgccggc
     3361 ggggtcggcc gcgccgtgca cgcgccggag ccgccgggaa ggtagcccgg agaaacggcg
     3421 taataggtct gccggaacca ctcgttcgca atgaaccagg cggcggccga ggccgttcct
     3481 cccgaggtgt aggtgccgct gccggaagat ccgttcaggc tgaaccgcgt cgcatcgatc
     3541 accgtgacgg tccacgtgcc gttcgccgcg ccgttgccgc tgacgttcga gatggtgacg
     3601 gccgcacccg tcggaaaatt atggggcgcg ctcgtcgtaa ccacgatcgg cgaggcgttc
     3661 gaagccgcgg tgatggtcga gtccgcggcg ctcgtcagcc ggctcacagt gaggtccggg
     3721 atggtcacga cgacggtgcg atcgctgcag ctggacatgc acgagatggg cagggtcgca
     3781 tcgtaggtca ccgtcccggc accggcagcc gtcaggctgc cgtggatcga cggcgacgag
     3841 accgtcgcgg acgcgccgtc gatcgtcgtc gttatcgtcg agacgtcggg cagggtcgcg
     3901 agcgaggtgc cggctttggt tccgacgctc gagctgaggc tcacgcgcgg gaagaagatg
     3961 atgtcgctgc cgaagaagtt gtcgctccgc acacgcaacg agcactgcca cccggtcgac
     4021 ggcggcggaa atggcgaact caaagcgacc gatacgcaag agttgatatc gacccgcgcg
     4081 accccgctcg acttggtcac ggagctgccg ctccacggat acggcgtcgg cgtgttcgcc
     4141 agcgtgctta tcggcagcag cccagcggcc tgcgaggcgt ctcccgagta gtccggaccc
     4201 gatgccgccg cttgcggccg cgtcgtcgcg gggccgttcg tgccgggacc tggcggcgca
     4261 ggctcggcga attttgcggg aaacggaaac gctccccact gattaaagta ggccacgaga
     4321 tacggcttga cgtcgcgctc gatgcgggcg ctcaccaccg gctcgacggc ggccatgaga
     4381 tccgcctgcg tgatggcgac caggcgatcg ttcagggcat ccgtaggcag ggcgttcgtc
     4441 gtgaatatga aattgacgct atcgttggcg ttgaatccct cgagataggc cgccgcgctg
     4501 ttgtgcccgg tcacacggtt ctgtccttgt gtcacctgcc cgggcgcaat gatgacggcg
     4561 acgatattgc tgccgctcgc agtgcccgtg accgtcagct gaccgggcgt atcgctgttg
     4621 atgactgtcg tgccatacag cttgcgaaag ttgcgcgata ccgcatacca gaggcgttca
     4681 ccgctcgagt cgcggagatc ctccgtgccg atggtcttcc agggaaggcg gccgatcaga
     4741 ctgatggaat tggtaatacc ggcgccgacg caatcggcat cgccatcgtc atcctggtcc
     4801 ggacacggca atccgcccgg ctggtttggg gactggttct tgtacagctg ccattgctcc
     4861 gacgcggcgt aggcgatgag cgcggacttc gccttcagca gtgcgctcat cgtggtctgc
     4921 tcccgggcgt tcgatatctg gctgctgctc ttgttgagcg cgctcaccag caggtagctc
     4981 gcgatgagaa cgaccagcga gaccacgacg atgagcgcga aaccgcgctc cctgggcgct
     5041 cgcttgtgaa cgcccgatgc cacgcgggac ctcacggtgt cgggttacgc tttaccacga
     5101 tccgcccgcc gccgagctcg tccctcacct cgccgcgcgt cttgtcgagc gtctgcccga
     5161 tcttgagatc gaccggcggg tcgccttcgt ggccgccgat cgtgacgcgc gacgggtcgg
     5221 gcgtgcgcac gctgtcatat tgcggcacgc cgttgatcca ggtggtcgtt ctccccgacg
     5281 agcgcgaaac gtggccgttc accgtcatgc tgctttccac ggcggcggaa gcgctttcct
     5341 ggacgttggc ttcgcgccgg cggtcgagct cctggcgctg ttgcggggtg aagaacaggc
     5401 gcccgatctc ctgcgccgcg accggcgcgg cggctcccgc catgcagcac gcgaggacga
     5461 ccgttgcgag ggcgcgcgcg cgcgtcacga ggcggtctcc tgatcgaccg tgatgaggtc
     5521 gatctggcaa tcggatctga ggcgcggcat gagcggctgt cccggcgata ctgcgccgcg
     5581 ttcgaggcgg ttgatgccgc agtgacgcac cgaaacgtag aagcgtcccg tggcgcggag
     5641 gtcggatagg aagttgagca ggtcctcctc gtgcaggagg agcatgtcga gcttgacgcg
     5701 gctcacgacg aagtcgccac ccttgatcgg gacgatcccg ggatagtcga gtcgtttctg
     5761 cggctcgatg ctgtacttga tctcgaacag cttgcgctcc gacttgatcc gggcgatcgc
     5821 gtcgatccag tcgagacggt tctcgggccc gatcatcccc tgccgctgca tgcgctggta
     5881 gaagaccaag ctctcgcgga tctcgcgctc ctcttccgac accttggata cgcgttcctg
     5941 cgcggcgctg cgctggccgc gcgcgttctc gagcttgtcg gttgcctggc cgaggtactg
     6001 gttgctcgcg acgacgaacg cgaggccggc gccgagaagc acggccgcga tggctgccgg
     6061 caggcgcaag cgtttcatgt cctcgcggcg caggttcatg tctcgagccg cctgctgatg
     6121 accaccgaga agcgcgggat ctcgaagccc cgcgctgcgc cgatgtcgcc cgagatgctc
     6181 ttctccgcgg ccaggtcgaa cggtagcttg gtggagacga tctccacgcc gggccggctg
     6241 cgcagcgcat cgacgaactg attgaccacc atggaaatgt tgcggtagtc ggacgcctgc
     6301 tgcacgagga ggcgtccgcc gatctcgacc aactggacgc gcggctcgcc gccgccgggg
     6361 gcctgcgcgg ccggcgccgc gtctcgggcg ccgggttgct tcagcggcgc gatatcccat
     6421 tcgatgcgct cgacctcgat ctgcggtgcc gaagcgagcg cgcggctgat ttccgccaga
     6481 atgtcgcgga catgggcatc ctgccgcatc aaggtctgaa agttcctgac cgtgagcttc
     6541 agcacttctg ctggaacggg cgtcttcggg aagttcgcct gcatccgcgc gtactgctcg
     6601 gacgccgtcg actcggcgat gcgctcggac tgcacggccc gatcgacgta gtcggcctgg
     6661 atgactttca tcccggagac catgaggcac agtgcgaaaa ccgcggcccc cgacaggatc
     6721 agcgccacct gggtccgcca cagatcatag aagcgccgca acgcgtcgtc cgcgaattgc
     6781 gcaaggaagc gcgcgcgcgc ggcgacgtgg aggtagagac cttcggcaag ggcgcggctg
     6841 ggggccgatt tcaagccgac gctgcgccgc gcgaaattca gatccacgag ccggtagcgc
     6901 aattgggcgt tgctgacgca ggccccctcg tacagcgtcc gtgactcccc gggcacgaga
     6961 accacgacgt ccagcgtcgt gctgtcgcgc gacagaatgc ggctgttgag taggtattga
     7021 tggaaacgcg ccgattcggc cgcgcacgcg tcggcgagcg cgttcggatc gtcccgatcg
     7081 accttgctga gacgcgaaaa ccgcgccttg ccgttgtcga cgtaggtttg ccgcaggccc
     7141 gcctcctgga gacttacgat catgtattgc cttgacttga cgccgaggcc cctgcacaac
     7201 aggggtgcga gcatcgcgat cgaatacacg ccggcgatcc gggcctgaat tgcgcgcaag
     7261 gagccgagcc acggttggaa ttgctgcgtg ttgctgaagg aggcgtagag gatgcgttcc
     7321 tcccgccggc ccgtcatctc ggtgccgagc gacagcgcca tggcgaggct ggtgtcccgg
     7381 taccgctgcg cgagcttccg gacgagcagc gcgcgccggt caccgccgcg cagatacggg
     7441 atgttctcct ggaagaagtc ctcctcgacc agatccgcga cgatgtgaaa aaggctgtcg
     7501 gatgcgcgcg acacgtactc cgagaaggcg gcggcccctt cgtctccggg cttgaacgtc
     7561 gcttcctcga ccagctcgcc gcgtttccag aggtacgcga ccagctcgtg cgaggtcagg
     7621 tacagcagac gcttgtcggg cagcatcaga tcttgatttt cgaaatgacg tcgtagatcg
     7681 gcccgagcac tgaaagcatg acccagccga ggatcgcccc gaggaagatc gtcagggcgg
     7741 gctcgatcat cacctgcact ctggcgatcg actcgcgcac ttcccggttg tagaagtagc
     7801 tcacgttatt cagcgctacg tcgagcccgc ccgtcgcctc gccgacgcgc agcatgcgca
     7861 cgacgagtgg cgggaacagc cccacgtcct ggaacgccgt cgtgacgttc ttgccgtctg
     7921 cgatctgctg gcccgcgatg cgcagcccct cctggatcac gaggttcccg gccgtctcct
     7981 cggagctgcg tatcgcctcg agaatcgtga tcccggaagc gtacatgagc gcaaaggtgc
     8041 tcgcgaaccg cgagagaatg atcttgcgca agatgttgcc gagatacggc agcgtgagct
     8101 ttagccggtc gagctggtag cgcacgagcg ggttggtctt cgccaacacc ttcaccagga
     8161 ccgcgaccac gacaggtgcg agcagcagag cccaccagta atcgacgaaa aaagcggaaa
     8221 cctggatcag catgcgcgtc tgccacggga tctcctgccc catgttcctg atgaagccga
     8281 ccatctgcgg caccaggtag accatgagga agaaggtcac catgatgacg atcgacccga
     8341 cgaatgccgg gtacatgatc agcttcttgg tctgcgcggc gagctcgtcc tcccatttca
     8401 agctctcggc caggctcttc agcacctccg ggaggcgccc cgtggtctcg ccggcacgca
     8461 ccaggctcga gaacaccggc gagaacaccg cgggatagtc ggtcatcgcc tgcgacaggc
     8521 tcgccccgcc ctggatgctc tccaccacgc ccgagacgac ctcgcgcagc cgcaggttct
     8581 cggtgctgtc gcgcaggtcc atcagcgcgt cgaccagcgg gacacccgag ttcgagagct
     8641 gctcgagctg gaagcagaag ttgatgagat cggcgcgctt gatgccgccg ccgcgcagca
     8701 gtccgccgcc gggcttggtg ggagcgccgc ggatcaggtc gagacccatg cgcttcaggc
     8761 gcatctccag gtccacgatg ttgatcgcct cgatctggcc catgctcgac ttcccccgac
     8821 cgtcgatcgc cttgtagatg aagagcgcca tgcgattccc ggggcggtga agcgcgctac
     8881 atgcgatcgg tgaggtcgac gacgcggatc agttcctcga gagaggtgga tccgtcgagc
     8941 acccgccgca cgccgtcgtc ggcgagcgtc cggaacccct ttgcgaccgc ggtcttctgc
     9001 agctcgcgcc cggtcgcgcg gtgcgccacc agctcatcga gctccgcatc catcttgagg
     9061 atctccatga tcgccatacg gcccttgtaa ccctggtagt cgcactggtc gcagccgacg
     9121 gctcggtaca acttgggcga gcgctcgccg tcgctcccga gcagcgcgcg ctcggcaggg
     9181 gtcggctcgt gcggctgctt gcagtgcttg cacagccggc gcaccaagcg ctgcgcaacg
     9241 atgccgatga tgttgccggc caggatgtcg ggcacgatgc cgatatcgag cagccgcggg
     9301 accgcgccca ccgccgagtt cgtatgcagg gtcgaataca cctggtgacc ggtcatcgag
     9361 gcgcggaagg ccatcgtggc cgtgtcctcg tcgcggatct cgccgacgag gatgacgtcc
     9421 gggtcctgcc gcatgagcga gcggatgccg ttgccgaaat ccatcttcgc cgcctcgttg
     9481 atcgaagtct ggcggatgag cgtcatcggg tattcgaccg ggtcctcgag cgtcatgatg
     9541 ttgagcccgt ccgtgttgat atggttcagc acggagtaca gggtcgtggt tttgccgctg
     9601 ccggtcggcc ccgtgaccag gatcaatccc tcgggccggg cgatcatctt cttgagcagc
     9661 gcgagttgct cctgcgcgag ccccagcccg tcgagcggca cgatcccctt ctggcggtcg
     9721 aggatgcgca gcacgatgtt ctcgccgtgc gtggtcgcct gcgacgacac gcggaaatcg
     9781 accgcgcggc cggtgagcgt gagcgagatc cggccgtcct gcggcgcgcg cgtctcggcg
     9841 atattcatct tcgacatcac cttgatgcgc accgccatcg ccggccagta ggtcttgtgc
     9901 agcgaccgga tctggcgcag cacgccgtcg atccggtacc ggatgcgcag gaagttctgc
     9961 tcgggctcga agtggatgtc ggaggctgcg cgctcgaccg cgtcggcgag cagcgcggag
    10021 atcagccgca cgaccggttg gctgtaggca tcgcccgagg cctgcaggct ctggtagtcg
    10081 atctcgcccg tttcgatctc gtgcaggatc ccgtcgatcg agaagtcatg cccgtagtac
    10141 tgctcgatgg cgcgcgcgat ctcggtctcg cccgcgagcc gggtctcgag ctcgaactcg
    10201 ccccgcaggt gagcgcgcac ctggtcgagc gcgacgatat tgttcgtgtc tgcgagcgcg
    10261 atgatgagct tgcgcgtctc gcggtccagc gccacgggga aaatgtgatg ccgccgcgcg
    10321 aagtcgcgag gaacgagctt cagcgcgacc gagtcgacga cgatctgcgt gaggtcgacg
    10381 ctctgcaggc cgagcttctc ggagagcgcg tcgcgcagcg tcgcctcgga cacgaagccc
    10441 aggttgacga gcagcttgcc gaccggctgg ttcgtcttga gctgctcaag gagcgcgatc
    10501 cggagctggt cctcggtcag gatcccctgc gcgatgagga tctggccgat gtggcgcttg
    10561 accgccggcc cgagctgggc cgacatacgg ggcgagttca tcggcgccct atctctgctg
    10621 cagatccaga gcgcgcgcgg cggcgagcgc gcggtcgaaa gtcgcgctgc gccggtcggc
    10681 gagcgccagc gagcgccggt agaactcgac cgcctgcggc ttctgatgca ggtgatcgag
    10741 gctcacggcc agattgaatg cgtaatccgg gttctcgggg tcggccgcaa gcgcccggaa
    10801 gtacgcctgc tgcgcctcgc cccagcggct ttgcagcgcg tattggttgc cgagcgcgaa
    10861 cagcagttgc ggcgagtccg gctggctcgc gagcaggttc ttgagccggc tctccgaggc
    10921 caccgggtcg gcctggttga gcgcgagcag accggcctgc gcgtagccgt cccgcgggtc
    10981 gagctccagc agccgcaaat agcgagcctg cgccgtctcg tagttgcgca tcctgagatc
    11041 gatggccgcc atcccgagca gcgcgtcgtg attgctgggt tcgcgctgca ggatgccctg
    11101 gtagatctga cgggccgtct cgagatcgtt cgcctggaaa gcttcatacg cgcgctgcag
    11161 ctccggatcg acctgcatcg aggccgggtt gatcgcgatc gactggcgcg ggccaggttc
    11221 gggtgcgtaa acagggtcgc ccgcctgggg cgccgacgct gtttgggcgg cgcgcgacgt
    11281 gggcacgaaa ggtcgcggcg cgggagcagg cttgggtacg gcgggagtgg ccgagcgaac
    11341 gcccggggac gcgaggcgct gctgcggtgg cgcgctgact ggcaccgggg tcggcccagg
    11401 cgccggtaca gccgacacag gcgccgcggc ggtcgtcgga ggcagcggcc gctgcaccgc
    11461 tgctgccgcc gcgatgcgct ccttcgcgtc gcgcgtggcc tgggtgttgt acgagtatcg
    11521 gggttgaagc tgccaccaca cgtagccgcc gtatgcgacc gcgaccaggc cgagcgcgat
    11581 cagcgtggcg tagaacgggc gtcgcgggtt gtaatcgatt tccttcacct cgaacagccg
    11641 tcgggcggcg tcctggttgt cagcgggggc gcgctcgctg cgcaatccgc tggggcgcgg
    11701 cgcgggctgc ggatcggcga ctatttcgag ctccaggtgc tgcggcttgc cgctctcgcg
    11761 gacttcgccg gacggcgatt tcgcagatgg caagtcgtca ctgtgaatct cgacgtgctc
    11821 gccgatgtcc ggcagctttt cgcgcgtgat gacggacttg gcggcctcgg gcgacggctc
    11881 cggatcgcgc gccggttccg tgccgccggc gcgctgcttc gcgagctcgg ctttcttgag
    11941 ggcttcgagg agcaggctca tggcggcgac agggggccgc ccagcggcgt ctgcttgccc
    12001 gggttcatgc ggccgatgaa gttctcgtcc ggcacgctgc tgcggaaagc gcgatagtcg
    12061 ccgtcgatac tcggatcctt gacgaccacg gggcgcagga agacgaccag ctcggtcttg
    12121 acgttcgcct tgttgcgctg ggtgaagaag cccccaagga gcgggatctc cgcgagcagg
    12181 ggcacccgat cctgcgtgtc gctgatggaa tcctggatca ggccgccgag caccgcgatc
    12241 tgaccgctgt tgaccttcat catcgattcc atctcccgcg tttgcacctc gggaatgcgg
    12301 cttacgatgg gctggcacgc ggcagccgcc gtgggcgcag agccgcatgg actggcgagc
    12361 gacggattcg gatcgttcac gaagctgatg atgcgcgaca cgctgggctt cacgttgagc
    12421 aggaccgtgt cgctgtcgct gatctggggc gtgacgttca tgacgaagcc gaccggcacg
    12481 acgttcggcg tcgtcgtgaa ggcggtggtc acgaccgttt gcgtcgccgt ctggttcgcc
    12541 tgcaccgtga aatagacgcg gttgtcgacg accttcagga tggcggtctg gttgttgatg
    12601 accgagagct tcggactcga gagcacgcgc acggtgccga aggactcgag cagccggatg
    12661 gtggagctga tgccgctcgc gacgtagccg aggacgaaca tgttcgtgct gacgccggcc
    12721 ggactgcccg cgctggtctg cgcgacgttg aacccggcgg gcccggttcg catgagcgac
    12781 caatcgatgc cttgctgata ctggttgttc agctgaacct cggcgatcgt cgcctcgatc
    12841 agaacctgac gcttggcgtt gaccaggacc tggtcgagga actcctggat cttttcgtgt
    12901 tgccgcgagg tcgcgcggat cgtcagcacc cccgcctcgg ggttcgcgat caccgaggcc
    12961 gcttcgcgga aagtcgcgct cggaacggcg atcggcggtt gtgaagaagg agccgcgccg
    13021 gcgacgccgc ccgcggccga cgccgcggcg gcggccgcgg actgcgatgg cgcagcgacg
    13081 gtccccgccg ggatgatctt gtcggtctcg cgcaggatgt ccttcacgtt gtcgacgagc
    13141 gtctcccaga accggttcgt cgcggtgctg cgtaccgtcg tgttggaggc gttgagcgcc
    13201 gacccgccgc ccgcggcccc cgggccgccg gcgcctgcgg tcgtcgtgcc ggaagagatc
    13261 tggctcgaga cgccgaccgt cgcgaccgtc gcgcggtcca tgttgacgta atcgatcttg
    13321 tagacgcgca gataaggcga gtcgggcatg acgacgaggt tgggcccatc gagctcccaa
    13381 cgcatgtcga cctgcttcgc gatgcgggcg agcagctgcg gcagcgtctg atcgatcgca
    13441 ttcagcgtca cggtgcccgc gatgcccgga tggatgtcga cattgagctt cgcgtcgcgc
    13501 gcgagcgcga acaagagctc ctgcacgcgc acattgttga ccacgacggt gtacgtctcc
    13561 gggcgcgcgg taggcttcgg cttcgggatg attgtcgaaa tctgaacagg cggggggatg
    13621 ctgcctgccg attcggcgtc cggacgaata tgtgtgtcgg cactgcgcat gggcgcgtgg
    13681 gtgcaggccg tgaccaccaa ggccatgccg gacatcgcca cgcgaactga cttcattttg
    13741 tgagggcccc cctgttcggc aacgcgcgct gcgcgccgtg ggcaatttcg ctggtcagta
    13801 tatcagacca gtctaagaat ttgatttaac gagctaaaaa aactccttta tcctcattgc
    13861 cgattctggc tgcgcatgcg ctcgaagctg cgcggataag gtcccagggc gcgcagcagc
    13921 gggggcagcc gattcgcggc aaccagcgcc tcgcgcgcgt ccgcataatt cccaaacgcg
    13981 acgcggtagt gctgcttccc cccgaatttg acgctgtaca ctaggagctc gtctgccgga
    14041 accagtttcg aagccttaag caagaaattc tccaagttct ggacgtcctc cgcctccacg
    14101 gtcaacaact gaatcgcctg acgctccccg ggcgccgcat cgagcagccg ctgggtagct
    14161 gccagccggt cccgccacag cgcgaccgca gtcgtgtcgg tccgtgccgg taccccggcg
    14221 tcggtaacca cgagcttagc gtcttcggta actttcgggt tggaaaattc ggtcgtgacc
    14281 ggaaccggct tcggtgcgga tgccgaagcc gctgcagcgg gagccggggc ctgggcggca
    14341 gggaggggcg cgacgctggc tggcgagcgc acggtcgccg ccggctccgc ccgacccggg
    14401 tcggcgagca gcgcatgcag catccagccg agggcgagtg aggtgacaag cgctccgacc
    14461 gcggccgcga ccctgccgag ccggcgggcg ggacggtaga agttcgagtc gccgatggcg
    14521 cgacggactt cgcgcggacc gaccgcatgc gagcccgctg cgaacgcggc caggagcgcc
    14581 ttgtcggcga ggatgttgac gcgccgcgtc aggccttgag acgcctgcgc gatcagctca
    14641 agcgctggcc gcgcgaagac attgggcccg cgatagcccg cggcgcgcat ccggaagtcg
    14701 acgtagctgt cgacgtcttc gcgccgcagc ggatccagcc ggaagctgtg cgtaatgcgc
    14761 tccttgagct ggcgcatgtc tgccgtatcg agcaggtcgt cgagctcggg ctggccgaac
    14821 agtacgatct gcagcagctt gtggcggttc gcctcgaggt tcgagaggag acggatctcc
    14881 tcgagcgttg gcttcggcat cgcgtgcgcc tcgtcgatca gtaccacgac gcggcgtccg
    14941 tccgcgaaca gggcgatgag gcggtcctgc agagcgcgca gaaggcgcgc ggagcgcagt
    15001 tcgcccatgt cgaagccgag ctcgtcggcg atggcgagca ggatctcgtc ggccgacagc
    15061 gacgggttgg cgaggaaaac agtctcgacg ttcttcggca gccgctccat gagcacgcgg
    15121 cacagcatgg ttttgccgct gcccacctcg cccgagacct tgacgatgcc ctcgtcctgg
    15181 cagatggcgt agaccagcgc ctcgagggtc gcgccgcggt tggcaccctg gaagaagaac
    15241 tcggtgttcg gcgtgatccg gaacggcggc tcgctcagcc cgaagtggtc caggtacatg
    15301 gaggggccgc tcgagaacgt ccgcggtgtt actgctgctt ctgcagcgat tccatccggg
    15361 agtacagcgt ggtccggttg atgccgagca tcttggcggc ctggctgacg ttgccgcggg
    15421 tgagcttcat ggcggcctcg atgtagccct gctcccaggc tttcagcatc tggtcgaggt
    15481 tgaagctgcg ctcgcgctgc agatgacgct gcgcctgctc gaccagcgtc cccggatcgg
    15541 ccggcatccc gccgggcgtc gcctcggcga gcgcttcgag gtcgaattcg ggctccagat
    15601 cggcgggcgc gagcttgcgc cccgcgtact tggtcgtgag gcggataaca atgttgcgca
    15661 gctcgcggac gttgccgggg aagtgatagt cgcgccaggt ccgctgcgcg tcgtcatcga
    15721 gcgtgaacgg ggcgaccttg gcctggtccg cgtagaactt ccggaaatgc tcgagcagcg
    15781 ccccgcggtc gtcgtccatc tcccgcagcg gcggcacgct gatggtgaac accgaaagcc
    15841 gatggtacag gtcggcgcgg aaattaccct tgcggatctc ctgccgcagg tcgcggttgg
    15901 tcgccgcgat cacgcgcgcc ttcgagaagc gctgctgcgt ctcgccgacg cgctggtact
    15961 cgccgttctc gagcacgcgc agcagcttcg cctgcagctc gagaggcagc tcgccgatct
    16021 cgtcgagaaa cagcgtgcct tcgcccgcgt cctcgaaata gccggacttg ttctgcgtgg
    16081 cgcccgtgaa ggcgcccttg gtgtagccga agagagtcgg ttcgaccagg gtcggcgcga
    16141 tcgcggcgca gttgagtgcg aaccagggtt tgctccgccg ccaggacagc cgatgcaaca
    16201 tgttggcgac cacgtctttg ccggtccccg attcgccttc gatgagagcc gggaacggcg
    16261 agtccgcgta ctgcgacacc tggctcttca gcttctgcag cgcggggctc ttgccgatca
    16321 tcggatcggc ttcgacggcc ttgtccttgg agatctccgc cgcccggaac tgcagcgcac
    16381 cgagcaggac ctgcttgagc ttctcggcgt cgcacggctt ggtgacgaat tccgcggcgc
    16441 ccagcgtgcg cgcgtgccgc gcgttggcgg aatcgttctg gcccgagagc acgatgatct
    16501 tcatcgtcgg ggagtgcgcg agcaggtcgg tgaccagctg gaagccctcg tccgggcggt
    16561 gcggcatcgg cggcagcccg aggtccacga gcgcgagctg cggcgcgtcg tcgagctggc
    16621 gcagcaggct caccgcgtgc tggcgcgagt cgctcaccat gatctcgaag tccttgccga
    16681 gcgcgtagga cagcgtgtcc gtgatgagcg ggtcgtcgtc gacgatcagc aggctcggct
    16741 tggcgtcagg catggcatcc gccattcgtt tcttctatac acctcagaaa ggcgctttcg
    16801 aggctgtcca cggaatagcg ccggagcagc tccgacggcg ggcccacata ggccagcctc
    16861 ccttcgtgca gcacggccat atggtcgcag atttcctcga tgtccgcgag cgaatgcgca
    16921 ctcaaaaacg cggtccggcc ggcagatttg cgctcgagca gcacctgttt cacgccggcg
    16981 cgagcccggg ggtcgagacc gctcatgggc tcgtcgagca cgtacaggtc ccgcgccgac
    17041 gcgaggcagg cggcgagccc gagcttctgg atcgttccct tggagcaggt gcgtaccggc
    17101 ctgtcgagtg tcgcgcgctt cagctgcaac gcgtcgagta cgacctcctc gccgtcgccc
    17161 gtcccgccgg cgagacgccg catggcggcg aggaactcgc gaccagtcag gaaatgcggc
    17221 ggcacgaaat tctcgggcag gtatgccagg cgcgcacgcg actcgggacg tcggtggcca
    17281 ataccgtgca gctcgatggt gcccgcgtcg atgtcgcaaa aatcgagcag gcatttcatc
    17341 agcgtggtct tgccggcgcc gttgacgccc gcgacgccga cgatttcgcc cgcgccgatc
    17401 tccaaatcga tcccgcgcag cgcggtgatg ctgccgtagc tcttgccgac cccctggaat
    17461 cggactaccg gctggtgcat gggacctcgc cagccccgtt ggagtgcgcc cgatgataac
    17521 gcgctttgac tgcgccgcga agcatcatcc agagctgcgc tcgacccggg agcgaccaga
    17581 cgcggttgta cgctagaatt gccgcttttc cccgatcccc cgatcgtgcc cacggaaaat
    17641 ctggtcgaaa tcgagaacgt cacgttcggc tacgatcagc gcaccgtact gcacggcatc
    17701 aatctcaccg tcccgaaggg cgccgtggtg gccatcatgg gcctgtcggg gtgcgggaag
    17761 acgacgctgc tgcggctgat cgccggcgcg atccgcgcca cgcgcggcga cgtgcgcgtc
    17821 gccggcgagc gcttgaacgg gctgtccgac acgggcctgt tcgccctgcg ccggaagatg
    17881 ggcatgctgt tccagttcgg ggccttgttc accgatatgt cggtcttcga caacgtcgcc
    17941 ttccaggtgc gcgagcacac ccggctgccc gcggcgatcg cgcgcgatct cgtgctcatg
    18001 aagctcgagt cggtcggcct tcggggcgcg gccgacctca tgccctccga gctctcgggc
    18061 ggcatggcgc gacgcgtcgc gctcgcgcgc acgatcgcgc tcgatccgtc gctcatcatg
    18121 tacgacgagc cgttcgccgg tctcgatccg gtgtcgctgg gcgcgatcgg acagctcatc
    18181 cgcaagctca acgacgcgct cggcgccacc tcgatcatcg tcacgcacga cgtggcggag
    18241 gcgctcgaga tcgtcgacta cgtctattac atgtcggccg ggcgcatcga gttccgcggc
    18301 acgccggccg aggtgcgcgc ctcgcgccat ccgtcgctcc ggcagttcgc gtacggcgag
    18361 cccgacgggc cggttccttt ccactatccc gcgcccgact accgcgcgga cctctcggcc
    18421 ggatcggcct gatgtcgcgc ctcgccgaaa tgctgcgtgc gctcggcgcg cgggtggtga
    18481 cccagtgggt gtggcgcgtc ggtttcgcgg gccgcttctt ctggctcacg ctcgccggat
    18541 cggggacgag cttccggcgc ttctcgctca ccatccggga aatctatttc gccggggtcc
    18601 tctcgctcgt catcatcctc gtgtcggggc tcttcgtcgg gatggtgctt ggcctgcagg
    18661 gttacgacac gctggtgcgc tacggctcct ccgaggcgct cggcgtcttg gtgtcgctct
    18721 cgctcgtgcg cgagctcggg ccggtggtcg ccgggctgct gttcgcgagc cgcgcgggga
    18781 gcgcgatcac ggccgagatc ggcttga
//
LOCUS       DATDMW010000137         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2407845, whole genome shotgun sequence.
ACCESSION   DATDMW010000137 DATDMW010000000
VERSION     DATDMW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2407845"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VI319_05935"
     CDS             complement(<1..399)
                     /locus_tag="VI319_05935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6863421.1"
                     /translation="MRRLLALAAALVLVDTMLYAALTPLLPHFLHEFGLSKSRAGILV
                     AAYAGGALVGGFLGGLAATHFGSRRAVLAGLALMGLSSLGFAFADTFWTLFGARLLQG
                     LGSGFTWSGALAWLLAVAPRARRGELLGPAM"
     gene            460..978
                     /locus_tag="VI319_05940"
     CDS             460..978
                     /locus_tag="VI319_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6863422.1"
                     /translation="MNAASPPLPATVASRPGFAEVVAGQLDAVYAYVVYLTGDRSAAE
                     DIAAETFEKAFRTWRRFDPRRGTPRTWLCAIARGIALDWLRAEARRRRREETYARDLE
                     LVGELGDGLPGPVEEALRRLSPGEREVIALRVLLELDGPSAARVLGISQTACSTRLSR
                     ALKRLEERIGDE"
     gene            971..1948
                     /locus_tag="VI319_05945"
     CDS             971..1948
                     /locus_tag="VI319_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY6863423.1"
                     /translation="MSELTIENVLRVHAPQAPQALRQRVLALEPVPRRRRWALAVVPA
                     AVAAAVVAAVVHGVVGSSSPKQAGRAFDRALPAQGNGAVASAPERKALTPSVGAASRL
                     QRTDASLQVRVSDVQKLTQTTAAATRIATSLGGYAQSVVYRAPAAGEGAAYIELRVPA
                     QNVKTALTRLAALGTLVSQQLSVQDLQHDFEVETAQIAGLRRTVAALQTALRNSSLPE
                     AQRVLLQIKLAESKRALFQRLHGRKGTLAAGTTARISLVLSTQKSVVPAVHHRGRLGR
                     MLHSAVGFLGLEGTIALYALIVISPIALVAALGWWLRGARRRRLEALVM"
BASE COUNT          333 a          818 c          822 g          282 t
ORIGIN      
        1 catcgccggc ccgagcagct cgccccggcg ggcgcggggc gcgaccgcga gcagccaggc
       61 gagcgcaccc gaccaggtga agccgctgcc gagcccctgc aggaggcggg cgccgaacag
      121 cgtccagaag gtgtccgcga acgcgaagcc gagactcgag agccccatca acgcgagccc
      181 tgcgagcact gcccgccgcg agccgaaatg ggtcgcggcc aggccgccga ggaagccgcc
      241 gacgagcgcg ccgcccgcgt aggcggcgac gaggatcccc gcgcgcgatt tcgagagccc
      301 gaactcgtgc aggaagtgcg gcagcagcgg cgtcagcgcc gcgtacagca tcgtgtcgac
      361 gaggacgagg gcggcggcga gagccagcag acggcgcatc gatttcaacc taccctgagc
      421 ggtgtgacga atcgcgggcg cggcgccgta caaacgggcg tgaacgcggc ctcgcctccc
      481 ctgccggcaa ccgtcgcctc ccgccccggc ttcgccgagg tcgtcgccgg gcagctcgac
      541 gccgtctacg cgtacgtcgt ctacctgacc ggcgaccgtt ccgcggccga ggacatcgcc
      601 gccgagacct tcgagaaggc ttttcgcacg tggcgccgct tcgacccgcg gcgcggcacg
      661 ccgcgaacgt ggttgtgcgc gatcgcgcgc ggcatcgccc tcgactggct gcgggccgag
      721 gcgcgacgcc ggcggcgcga ggagacgtac gcccgcgacc tcgagctcgt cggcgagctc
      781 ggagacggac tgccgggccc ggtcgaggag gcactccgcc ggctctcgcc gggcgagcgc
      841 gaagtgatcg cgttgcgcgt cctgctcgag ctcgacggcc cgagcgcggc gcgcgtgctc
      901 gggatcagcc agaccgcgtg ctcgactcga ctgagccgcg cgctgaaacg actggaggag
      961 aggatcggcg atgagtgagc tgacgatcga gaacgtgctg cgcgtgcacg cgccgcaggc
     1021 cccgcaggcg ctccggcagc gcgtgctcgc gctcgagccg gtgccgcgac gccggcgctg
     1081 ggcgctcgcg gtcgtcccgg cggcggtggc cgccgcagtc gtcgctgcgg tcgtccacgg
     1141 tgtcgtcggc tcctcgtctc cgaagcaggc cggaagggca ttcgaccgcg cactgccggc
     1201 gcaggggaac ggggcggtcg cgagcgcgcc ggaaaggaag gcgctcaccc ccagcgtcgg
     1261 cgccgcgtcg cgccttcagc gcaccgacgc ctcgctgcag gtccgggtct ccgacgtcca
     1321 gaagctcacg cagacgaccg cggccgcgac gcgaatcgcg acctccctcg gcggctacgc
     1381 gcagtcggtc gtctaccgcg cgcccgccgc cggcgaaggc gcggcgtaca tcgagctccg
     1441 cgtcccggcg cagaacgtga agacggcgct cacccggctc gccgcgctcg gcacgctggt
     1501 ctcgcagcag ctctccgttc aggacttgca gcacgacttc gaggtcgaga cggcgcagat
     1561 cgccggcctc cggcgtaccg tcgccgcgct gcagaccgcg cttcgcaact cgtcgctgcc
     1621 cgaggcccag cgggtgctgc tccagatcaa gctcgccgag tcgaagcgcg cgctcttcca
     1681 gcggctgcac ggacgcaagg ggacgctcgc ggccggaacg accgcgcgaa tctcgctcgt
     1741 cctcagcacg cagaagtcgg tcgtcccggc ggtgcaccac cgcggccggc tgggccgcat
     1801 gctgcattcg gcggtcgggt tcctcgggct cgaggggacg atcgcgctct acgcgctgat
     1861 cgtgatcagc ccgatcgcgc tcgtcgctgc gctcggctgg tggcttcgcg gggccaggcg
     1921 acggcgcctc gaagccctcg tgatgtagcc ggtcggccgc gctcccagcg ccgtcgaagg
     1981 gcaacacagc gcttcctcgg gttatcggcg cgatgcgctt caatcgggca gatgctcgag
     2041 aagctgcgtc atctgctgag gcgcatggtc ccgcggggca cagagcaagt tcgactcaca
     2101 cggggggaac gagacgagcg tgaccgcgtg ctcgcgcacc gatgggagca cgtcaggcac
     2161 cggcaggaag agaagcccta gactcgccgg cactgcggcg gcgtgggcga acttccgtaa
     2221 cgggctctgc gcgtctagac cgggtcgttc gaccg
//
LOCUS       DATDMW010000138        18970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1037500, whole genome shotgun sequence.
ACCESSION   DATDMW010000138 DATDMW010000000
VERSION     DATDMW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18970
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037500"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VI319_05950"
     CDS             <1..359
                     /locus_tag="VI319_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863424.1"
                     /translation="AFVAIEAVLTDAFVDAGQHFALRQLAASAAAGNASAGGAILALA
                     AVVTIAVAAAYGALVALLARRYGGAGAWWTGVASGLTVYIVNFHYLLAPTLRWLGEVP
                     DRVSVLSPWYLVSLWP"
     gene            364..1416
                     /locus_tag="VI319_05955"
     CDS             364..1416
                     /locus_tag="VI319_05955"
                     /inference="COORDINATES: protein motif:HMM:NF021595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY6863425.1"
                     /translation="MLLSAEGTEFCGFGALSRLMNMGILVLDQAADLQFANPLAVELL
                     GGGGAEALKSRWPGLRGELGLVAERLRAATPARLVADTESGSLRLEVYSIEEEPCTGY
                     LVLVKDRRAADVLETDLLLASQMRSVPHLYRVLAHDLKAPLNAMQLTLELLSDPLSGP
                     AEPAREAKRQRYIGVLREEMRRLDRTLQTMLGENEPIGAAARTFDFRDLVRDIAALLT
                     PQARRQRVAIDLRVPEQPVEATAMRDRLKQAFLNVAINALEAMPAGGRLHLALSRDGD
                     GVVLECRDTGPGMKPEQLDEVFQLYYTTKKSGSGLGLYVARLVAESHGGEIRIDSRPG
                     QGTTVALSVPARGPAR"
     gene            1449..2765
                     /locus_tag="VI319_05960"
     CDS             1449..2765
                     /locus_tag="VI319_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY6863426.1"
                     /translation="MIRTQWFLIIGALLLLMALLGSLVKRLPLSMAMLYLLIGAGLGP
                     LGLGILRVDAVADSALLEPVLEIGVLISLFSAGLKLRAPMSDEPWKLPVRLAVVAMIV
                     SIALLALAAWALLGLPAGVAILLAAILAPTDPVLASDVQVEHPGDQHEVRFGLTGEAG
                     LNDGIAFPFVMLGLGLMGLHALGPLGARWLALDVGWGIVAGIAAGWLPGTALGRLVLY
                     LRRTMKEAVGLDEFLGLGLLALSYGLALLAHGYGFLAVFTAGVALRRVERKLSGGRSP
                     QELVVSAEPGAARESATDPKDAPAYMAWAVLSFNERLERVVEVGLVLVIGGMLPASAL
                     SLQAAALALLLFVVVRPASVMISLAGAGVPATHARLMAWFGIRGIGSVYYLVFAARHG
                     LPAAQAAGLADLVLGVVAASVIVHGVSATPLMRLYSGMQSRRPGSR"
     gene            3186..3416
                     /locus_tag="VI319_05965"
     CDS             3186..3416
                     /locus_tag="VI319_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863427.1"
                     /translation="MASAAITEDPTAWYRDEQRKLEKLQSEFRHWLETKEDIVRLADR
                     RDQLLALLDAKYGYRADAAEAAERNEADPLCP"
     gene            3624..3785
                     /locus_tag="VI319_05970"
     CDS             3624..3785
                     /locus_tag="VI319_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY6863428.1"
                     /translation="MLQLAAVFFVIALIAAIFGFGGIAAGAVEIGKILFFIFLVIFVV
                     TFVLGIMRS"
     gene            3862..4107
                     /locus_tag="VI319_05975"
     CDS             3862..4107
                     /locus_tag="VI319_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEY6863429.1"
                     /translation="MARSIEGTAADAGEMVRDVGNKIGARMQTARQQLTQAQSAVSDG
                     ARQAAEFTDEYVRMNPWKSLGIAAAAGLLIGILMTRR"
     gene            4116..4517
                     /locus_tag="VI319_05980"
     CDS             4116..4517
                     /locus_tag="VI319_05980"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY6863430.1"
                     /translation="MMVREEIAAGRAQAGGIIDSLKGIARTSVEIVHSRLELLVTELS
                     EEQSRLIELALVAALALLALFLGIVFAAFLVVAAFWDTPYRLWAPGIIALVLIAGAAV
                     LWTVFVKKAKAKGQAFKATLHELATDVEHLR"
     gene            4514..4852
                     /locus_tag="VI319_05985"
     CDS             4514..4852
                     /locus_tag="VI319_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863431.1"
                     /translation="MRLRSRELAERRERLQSRIAAQRLELERQLSDWRGPVHAFEVAR
                     HAGQRLRSQATALLAIASALLLISRGRVLGKMFLALRWARFAAKWWAFGRMLYRASGG
                     FGRGGAAHYR"
     gene            4869..5411
                     /locus_tag="VI319_05990"
     CDS             4869..5411
                     /locus_tag="VI319_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY6863432.1"
                     /translation="MANESAFITDIQTLRKRAREHIENGAITPGYAADRGTVIKLLNE
                     ALATEIVCTLRYKRHYFMAKGINAEAVAAEFGEHATEEQEHADQIAQRIVQLGGEPDL
                     SPQGLASRSHAEYIEGDSLTDMIKEDLVAERIAIDSYKEMVNYFGSQDPTTRRLMEQI
                     LAKEEEHAEDLVSLLEGMEG"
     gene            5453..5785
                     /locus_tag="VI319_05995"
     CDS             5453..5785
                     /locus_tag="VI319_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863433.1"
                     /translation="MRNVTLSFLIVALAAVSACNRTADQTPASGPQAKAPSTPPPSGS
                     TPPAEVNQPSSGATQPQAPSASTGTSHAAESPSKELSKQEESTGQPSPGTGGADHSTP
                     AREPGAKG"
     gene            complement(5817..6041)
                     /locus_tag="VI319_06000"
     CDS             complement(5817..6041)
                     /locus_tag="VI319_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863434.1"
                     /translation="MGSIAASFVRCVRCQRDFRSPVTFTTLEDFERAAEIGMIAQCPS
                     CFTIFECNGTNMYCELAARAEPASGAPSSG"
     gene            complement(6145..6888)
                     /locus_tag="VI319_06005"
     CDS             complement(6145..6888)
                     /locus_tag="VI319_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEY6863435.1"
                     /translation="MNAQESSWIRRVMTAGSFADLKDELAHAAAGLGFAHYALRARYP
                     NLRTGFNEISFDNCPNGWRRYYLKHGPGAESDPVRARAQQQTTPVLWRELMPQAPTLV
                     RKAREFGFATGVIFPTHGPAGQWSALTMISSQGGVDAERAILDTMAEAHLLAAYAHDA
                     CARIIRRRLHASVPVSQPVPEVALSRRERECLVLAARGKTAAEIGRSLEISERTVTFH
                     LTNARRKLGTASSPHAVVRAVSLGLIAAG"
     gene            7165..8859
                     /gene="phaC"
                     /locus_tag="VI319_06010"
     CDS             7165..8859
                     /gene="phaC"
                     /locus_tag="VI319_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY6863436.1"
                     /translation="MTGTAAFAAEEFYRAGQALFQNFFGAMAAQSGGGTGEPLEASAA
                     KLASLQAEFSRRHLALWQSALSRGADAEPDAPVDRRFSSPEWRSGPWFDYVRRSYEIN
                     ARFLADCVEAIDVQPLAKQRLRFATRQMIDALSPANFVATNPEAIRLALETGGESLTR
                     GVLNLIEDAHKGRVSTTDESQFEVGRNLAVTPGAVVHENELMQLIQYAPQTETVHERP
                     LVMIPPCINKFYILDLQPGNSFVRHAVEQGHTVYMVSWRNVGPEQGRYGWDDYLELGV
                     LRAIDVARDIAGADAVNALGFCVGGTMLGAALAVLAARGESRVASATYLASMLDFSDT
                     GDIGLFIDEASLAMRETAIGQGGIMPGRELAFVFSALRANDLVWSYVVNNYLKGRPPE
                     AFDLLHWNADSTNLPGPMYCQYVRRTYLGNELREPGRVSMLGSPIDLGAVKTPAYLFA
                     TREDHIVPWRTAYRSTGLLGGDLRFVLGASGHVAGVINPAAKNRRSHWIGGNLPPDPD
                     TWLQNAVEQRGSWWTDWSAWLAPHGGRRVQARKSLGSARHKPIEPAPGRYVKHRIA"
     gene            8875..10053
                     /locus_tag="VI319_06015"
     CDS             8875..10053
                     /locus_tag="VI319_06015"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY6863437.1"
                     /translation="MTDVVIVAAARTAIGKFGGALSKIPAADLGAHVIKAVLARARLK
                     PEQVSEVIMGQILTAGIGMNGARQASIRAGIPDMSPAMTINMVCGSGLRAVALAAQAI
                     KAGDSEIVVAGGQESMSLAPHVLMGSREGFRMGDAKLIDTMINDGLWDVFNQYHMGVT
                     AENVAKQYQVSRAEQDEFAAKSQQKAEAAQKAGRFKDEIAPVEIAQRKGPALAFDADE
                     YPKHGTTAEALAALKPAFDKAGSVTPGNASGINDGAAAVAVMSARTAEKLGLKALARI
                     KAYATAGVDPKIMGMGPVPASKLCLSRADWTIKDLDLMEINEAFAAQAIAVNKQMGWD
                     TSKINVNGGAIALGHPIGASGCRILVTLIHEMIRRDAKKGLASLCIGGGMGVALAVER
                     "
     gene            10172..10912
                     /locus_tag="VI319_06020"
     CDS             10172..10912
                     /locus_tag="VI319_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEY6863438.1"
                     /translation="MKQRIAFVTGGMGGIGTAVCRRLAKNGVRVVAGCLPGYDKKEQW
                     LDAMRAEGLSLYAAEGNVDDYESCAEMFYKIKSIVGPVDILVNNAGITRDGVFKRMSP
                     TDWYAVINTNLNSVFNVTRQVIEGMIERGWGRIVNMSSVNALKGQFGQTNYSAAKAGM
                     HGFTKALAQEVVKKGVTVNTISPGYVETDMVKAMKPEVLKSVIDQIPMGRLAQPDEIA
                     GLIGYLVSEEAGYVTGANISINGGLHME"
     gene            11027..11770
                     /gene="phbB"
                     /locus_tag="VI319_06025"
     CDS             11027..11770
                     /gene="phbB"
                     /locus_tag="VI319_06025"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY6863439.1"
                     /translation="MAQRLALITGGMGGLGETISTKMSDAGYRVAVTYSPSNKQSETW
                     IGEMKARGYAFRAYACDVADYDSAKRCVEAVVADVGPVDILVNNAGITRDMTFRKMGK
                     ADWDAVLRTNLDSVFNMTKQVCDGMVDRGWGRVINVSSVNGSKGAFGQTNYAAAKAGM
                     HGFTKSLALEVAKKGVTVNTISPGYIGTKMVTAIPKEILDSKILPQIPVGRLGKPDEV
                     AGLIIYLCSEEAAFVNGANIAINGGQHMA"
     gene            complement(11767..12309)
                     /locus_tag="VI319_06030"
     CDS             complement(11767..12309)
                     /locus_tag="VI319_06030"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY6863440.1"
                     /translation="MAHAPETLSGWQRAAIDTTIACAQTALAGAEQLLRLNIDAARRA
                     LEQHAQAVRALLGTSDPAELMRLRSRLAQQTMEQAASYAQEVYELVRETQAQLAQHAE
                     QQLSGLNDDLARRADESATPGAEVAVAAVRSSIAASAAVMENLNRATQQFADLSQATI
                     RAAATNLGRVGEPQAPGRRA"
     gene            12464..13075
                     /gene="phaR"
                     /locus_tag="VI319_06035"
     CDS             12464..13075
                     /gene="phaR"
                     /locus_tag="VI319_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY6863441.1"
                     /translation="MAEPVRLIKKYPNRRLYDTRTSTYITLADVKQLVLQNEEFQVVD
                     AKTSEDLTRAILLQIILEEEAGGAPMFSSDVLTQFIRSYGNAMQGMLRGYLERNMQFF
                     TEIQKRMQEQSKQLYGEPVKFNEDLWKQFMSFQAPAMQSLMSAYMEQSRNMFLQMQEQ
                     LQNQTRNMFSGFTFPGFPGFGAAAGGAAKAEGEEKKPDDEKKS"
     gene            13199..14206
                     /locus_tag="VI319_06040"
     CDS             13199..14206
                     /locus_tag="VI319_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEY6863442.1"
                     /translation="MFLAVLAALGAWVVVDQRSGPRAPGPASGGQPAGAARAPSEFRA
                     ELPERAPLQRAGKDPFSTRSWTPPPARRPAGPVAAAPPPVAPPLPYRYVGELVLPTGT
                     QVYLARGDDTFRVSEGETLDGQYRVESIKRGEMVFVHLATGQQQTMRFSLPGEDAEAQ
                     ARAIVRSADGAPRPAGAPAAVLPASSPTAPATASAGPAEPARLRWDGPASAKAGATFN
                     VALRMTSASEQIRSAPMEVRFDPKLLEPVSVRPGRYFGADRSNFGYRISGEGSIYIGV
                     SNRTPPSTANDAELLVLTFKPIKAGALAELNVAALNLQGTAGRPVAYTALGAFKTTII
                     P"
     gene            14339..15391
                     /locus_tag="VI319_06045"
     CDS             14339..15391
                     /locus_tag="VI319_06045"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HEY6863443.1"
                     /translation="MSAIPANAPAAGQRTVRPEFTASGAEYFRIWIVNLLFTLLTAGI
                     YSAWAKVRKKRYFYGSTKFDGSTFDYFASPRAILNGRIIAFVVFVVYAIAGDLHPQSH
                     VAFLAVGGLALPWLVVRALGFNARNSAWRGLRFDFTATTRQAITVYIGYFLIVVLTLG
                     FAYPWFVARQKAFVIGNHALGKSRFGCDMPARALFGIYVRAGLLMTVLVAPLVGLMTF
                     VMVKMPVPENLAWLKFTLPILPMYAAYVAVYAYSQARTQNLMWNSAYGPGMRFSSTLS
                     AVRLARIYIGNIVVAACSAGLLIPWAVVRTMRYRLEQFAVVLEGDPVHEASPALARVG
                     ATGQELGDFFNLDLGL"
     gene            15388..17646
                     /locus_tag="VI319_06050"
     CDS             15388..17646
                     /locus_tag="VI319_06050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY6863444.1"
                     /translation="MNVIAGDYYDGRTSVRHAVSAIVSNGRLKVVGRDVNLELDLRRV
                     RRSLRVANTPRWLYLPGGGALVTADNDAIDRMTRKRRYDRLLHRWESRPALAALAMVA
                     VIASLWLAFDRGVPLAVERIAERIPPTAEAALGEQTLRSLDQRLTQPSTLSRERQAAL
                     RERFTAMTQAAGDPTPYRLEFRSSKFIGANAFALPSGIIVMTDELVKLSRRDEEVLAV
                     LAHELGHVHHRHTMRRLLEASATGLIIAAVTGDVASATSLAAAAPAVLLQTRYSRDNE
                     READAYSIAMLQKSGLSPRNMATILGRLEAKHAGRRRGGLPDFLSSHPATKERQAQAL
                     AAARDDAPADDATPADGAVPMPARAPRGVIVDPVQRQVVELVEKRDYETLERLLSERQ
                     AAFERDPATSPVLDSSYRAFDKVDPASAPALDEWIARYPSSYAARAGRATFLLYRGQD
                     ARGTATFDETPPENIEAMRALLTRALEDAGRSLAMTPKPYISHRTLMTVARLSGRRKE
                     ALEHYEAAVRLAPESIDTRLDHMTLLEPRWGGSYAEMEKFAARSRAELKAPGGADRLA
                     AHVGEYRADESLRARDFAAALRDYDWALGLDPGAARSCGRAYALSELKRDDEALADVK
                     RVLAIAPDQRYCRELAPHVASRAANAGEAIAVMNLVIDADPSSTPALTQRGWRYQQQN
                     RPELAFPDYLAAAKLGDPWAELQAGKFLWNGLGVKADREEALGWLRKAAAHGDRDAKV
                     SLEQALAELARK"
     gene            17753..18745
                     /locus_tag="VI319_06055"
     CDS             17753..18745
                     /locus_tag="VI319_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY6863445.1"
                     /translation="MSLALAPLAASAWDRGDVQRFATLPSGAGHPEGIAADKDGNIYT
                     TIFDPTGAATNKHQMFVFDRQGRLVREVNIAGASHALLGLDFHPTTGALLVLDFGQGK
                     VLKVDPVTGASTVFTTVTGASGLNALTFDQQGNVYISDSFQGIIWRTGPNGSPTATVW
                     AQDPLLQPNGIPPFGANGLGFNKAFNTLFVANTANDSIIQIPVTPSATGPVAGPASAL
                     AYSVNGADGLILDADDNLWICANQSDEIVVLDKTGKVISKLGDFDGVDRDQAPIGLLF
                     PASPVRVGDWIYITNLSLDLRNVGGPQTIDSQWADEVQTQTLARIRARIRPNRE"
BASE COUNT         2991 a         6514 c         6604 g         2861 t
ORIGIN      
        1 ccgcgttcgt cgcgatcgag gcggtcctca ccgacgcctt cgtcgacgcc gggcagcact
       61 tcgcgctgcg ccagctcgcg gcgtcggccg ccgccggcaa cgcgtcggcc ggcggggcca
      121 tcctcgccct cgccgcggtc gtcaccatcg cggtggccgc ggcatacggc gcgctcgtgg
      181 cgctgctcgc gcgccgctac ggcggcgccg gcgcgtggtg gaccggcgtc gcgtccggcc
      241 tcaccgtcta catcgtcaac ttccactacc tgctcgcccc gacgctgcgc tggctcggcg
      301 aagtgcccga ccgcgtgagc gtgctgtcgc cctggtacct cgtctcgctg tggccctgag
      361 ggggtgctgc tgagcgccga aggcaccgag ttctgcggat tcggcgcgct gtcgcgcctg
      421 atgaacatgg gcatcctggt cctcgaccag gccgccgatc tgcagttcgc caatccgctc
      481 gccgtcgagc tcctgggcgg cggaggcgcc gaggcgctga agagccgctg gcccggcctg
      541 cgcggcgagc tcggcctcgt ggccgagcgc ctgcgcgccg cgacgcccgc gcgcctcgtc
      601 gccgacaccg agagcggctc gctgcggctc gaggtctatt cgatcgagga ggagccctgc
      661 accggctacc tcgtcctcgt caaggaccgc cgcgccgccg acgtgctcga gaccgacctg
      721 ctgctcgcca gccagatgcg ctcggtcccg cacctgtacc gggtgctggc ccacgatctc
      781 aaggcgccgc tcaacgcgat gcagctgacg ctcgagctgc tgagcgatcc gctctcgggc
      841 ccggccgagc ccgcgcgcga ggcgaagcgc cagcgctaca tcggcgtgct gcgcgaggag
      901 atgcggcggc tcgaccgcac gctgcagacg atgctcgggg agaacgagcc catcggcgcg
      961 gccgcgcgca ctttcgactt ccgcgacctg gtgcgcgaca tcgcggcgct gctcacgccg
     1021 caggcgcgcc ggcagcgcgt cgcgatcgac ctgcgcgtgc cggagcagcc ggtggaggcg
     1081 accgccatgc gcgaccgcct gaagcaggcg ttcctcaacg tcgcgatcaa cgcgctcgag
     1141 gcgatgcccg cgggcgggcg actgcacctg gccctgtccc gcgacggcga cggcgtcgtg
     1201 ctcgagtgcc gggacaccgg ccccggcatg aagcccgagc agctcgacga ggtctttcag
     1261 ctgtactaca ccaccaagaa gagcggcagc ggcctcggcc tctacgtcgc gcgtctcgtg
     1321 gcggagtcgc acggcggcga gatccgcatc gacagccgtc ccggacaagg caccaccgtc
     1381 gcgctcagcg tgccggcgcg cggaccggcg cgctgatccc cgtcccgggt cgcgcccggg
     1441 cccgtcccat gatccggacc cagtggttcc tcatcatcgg cgcgctgctg ctcctgatgg
     1501 cgctgctcgg atcgctggtg aagcgcctgc cgctcagcat ggcgatgctc tacctgctga
     1561 tcggcgcggg gctcgggccg ctcggcctcg gcatcctgcg cgtcgatgcc gtcgcggact
     1621 ctgccctcct cgagccggtg ctcgagatcg gggtgctgat ctcgctgttc tcggcggggc
     1681 tgaagctgcg cgcgccgatg tcggacgagc cctggaagct gccggtgcgc ctcgcggtgg
     1741 tggcgatgat cgtgagcatc gcgctgctcg cgctcgcggc atgggcgctg ctgggacttc
     1801 cggccggcgt cgcgatactg ctcgccgcga tcctcgcccc gaccgacccg gtgctcgcgt
     1861 cggacgtgca ggtcgagcat ccgggcgacc agcacgaggt gcgattcgga ctcacgggcg
     1921 aggcggggct caacgacggg atcgcgtttc ccttcgtgat gctcggactg gggctgatgg
     1981 gcctgcatgc gctcgggccg ctcggcgcgc gctggctcgc gctcgacgtc ggctggggca
     2041 tcgtcgccgg gatcgccgcg ggttggctgc ccggaacggc gctcgggcgc ctcgtcctct
     2101 acctgcggcg cacgatgaag gaggcggtcg gcctcgacga gtttctcggc ctcgggctgc
     2161 tcgcgctctc gtacggcctc gcgctgctcg cgcacggcta tgggttcctc gccgtgttca
     2221 cggcgggcgt ggcgctgcgg cgcgtggagc gcaagctctc gggagggcgc tcgccgcagg
     2281 agctggtggt gagcgccgag cccggggcgg cccgggagtc ggcgaccgat ccgaaggatg
     2341 cgccggcgta catggcgtgg gcggtgctct cgttcaacga gcgcctggag cgcgtggtcg
     2401 aggtcgggct ggtgctcgtc atcggcggaa tgttgccggc atcggctctg tcgctgcagg
     2461 cggcggctct cgcgctgctg cttttcgtcg tcgtgcgtcc ggcctcggtg atgatctcgc
     2521 tcgcaggggc gggtgtgccg gcgacccacg cgcgcctgat ggcatggttc ggcatccgcg
     2581 ggatcgggtc ggtgtactac ctggttttcg cggcacggca cgggctgccg gcggcccagg
     2641 cggccggcct cgccgacctg gtgctcggcg tggtcgcggc ttcggtgatc gtgcacgggg
     2701 tgtcggcgac gccgctcatg cgcctctaca gcgggatgca gtcccggagg cccggaagtc
     2761 gatgaatcct ttgcgatgtc gcgatagtgc gacagacgta aagcttgcac gatggcctgt
     2821 aagtcttact ggcacggccc ccagtcggcg catgccgtgg gggcgaaatg acgaaggttt
     2881 tctgcatcgt agatggcccg gcaggacttg gcatgaacgt tgctgatagt gccatgcgca
     2941 tgtaccgaag gtctgcaggt cccggttgtc cggtcttgtt cgtccgtcgg aaacttctca
     3001 gcaggaaacc gttcgatgag cgtcactgcc accacgaaga agcccgcctc cggcaagcga
     3061 gcgtatcggc gcgaggatcc cgagcgccgg cgcgcggagc gcaagtggtt cgatcccgcg
     3121 atgagacgcg agaaacacgc gcgccgaccc ggccagggtc acggtctcgc aggggagcgc
     3181 tagccgtggc gagcgccgcg atcaccgaag accccaccgc ctggtaccgc gacgagcagc
     3241 gcaagctgga gaagctgcaa tcggagttcc gccactggct ggagaccaag gaagacatcg
     3301 tgcggctcgc cgaccggcgc gaccagctgc tcgccttgct tgacgcgaag tacggctacc
     3361 gcgccgacgc cgccgaagcg gccgagcgga acgaggcgga cccgctctgt ccctgaagct
     3421 gctctgctgt aactcttacc gcgctcctgc aattccttcc ggggccccgc gcgccgcggt
     3481 cggggcggcg ccgaaataac ccttgtaaat gagccgggtg tcccgcccgg cgggacgccg
     3541 cgcgcgcggc acggagcttg cagtagaccg tgcggggcga acgggaggcg ggtggtcatt
     3601 cgcctcgcta caaggagatc aacatgttgc aactggctgc agtgttcttc gtgatcgccc
     3661 tgatcgccgc gatcttcggc ttcggcggga tcgccgcggg cgcggtcgag atcggcaaga
     3721 ttctgttctt catatttctg gtgatcttcg tcgtcacgtt cgtgctgggc atcatgcgaa
     3781 gttaggtact caaaccagga ggcgcaagtg gaaagcgatg tgatgaagag ccccgagggg
     3841 cttcagagaa ggagcgacaa agtggcacgc agcattgaag ggaccgcggc cgacgccggc
     3901 gagatggtga gagacgtcgg caacaagatc ggcgccagga tgcagaccgc gagacagcag
     3961 ctcacgcagg cgcagagcgc ggtttccgac ggagcccggc aggcagccga gttcaccgac
     4021 gagtacgtgc gcatgaaccc gtggaagtcc ctcggcatcg cggccgcggc cggcctgctg
     4081 atcggcatcc tgatgacgcg ccgctaggca ccggcatgat ggtgcgcgaa gagatcgccg
     4141 ccggcagggc tcaggcgggc ggaatcatcg actcgctgaa gggcatcgcc cggacgtcgg
     4201 tcgagatcgt ccactcgcgg ctcgagctgc tggtcaccga gctctccgaa gagcagtcgc
     4261 ggctgatcga gctggcgctg gtcgccgcgc tcgcgctgct cgcgctgttc cttggcatcg
     4321 tgttcgccgc cttcctcgtc gtcgccgcgt tctgggacac accgtaccgg ctgtgggcgc
     4381 cgggcatcat cgcgctcgtg ctgatcgccg gcgcggcggt gctctggacg gtgttcgtga
     4441 agaaggcgaa ggcgaagggc caggcgttca aggccacgct gcacgagctc gcgaccgacg
     4501 tcgagcactt gcgatgaggc tgaggtcccg ggagctcgcc gagcggcgcg agcgcctgca
     4561 gagccggatc gccgcgcagc gcctggagct cgagcggcag ctctcggact ggcgcggccc
     4621 cgtgcacgcc ttcgaggtcg cgcgccacgc gggccagcgc ctgcgcagcc aggcgacggc
     4681 gctgctcgcg atcgcgagcg cgctgctgct gatctcgcgc ggtcgcgtcc tcgggaagat
     4741 gttcctcgcg ttgcgctggg cgcgcttcgc cgccaagtgg tgggcgttcg gccggatgct
     4801 gtaccgcgcg agcggcggct tcgggcgcgg cggcgccgcc cattaccgat gaccgcaagg
     4861 agaagacgat ggcaaacgaa tccgctttca tcaccgacat ccagacgctg cgcaagcgcg
     4921 cgcgcgagca catcgagaac ggcgcgatca cgcccggcta cgcggccgac cgcggcacgg
     4981 tcatcaagct gctcaacgag gcgctcgcca ccgagatcgt gtgcacgctg cgctacaagc
     5041 gccattactt catggcgaag ggcatcaacg ccgaggcggt cgccgccgag ttcggcgagc
     5101 acgcgaccga ggagcaggag cacgccgacc agatcgcgca gcgcatcgtc cagctcggcg
     5161 gcgagccgga cctctcgccg caggggctcg cgagccgcag ccacgccgag tacatcgagg
     5221 gagattcgct caccgacatg atcaaggaag acctggtcgc cgagcgcatc gccatcgaca
     5281 gctacaagga gatggtgaac tacttcggca gccaggatcc gacgacgcgg cggctcatgg
     5341 agcagatcct cgccaaggag gaggagcacg ccgaggacct ggtgagcctg ctcgagggca
     5401 tggaagggtg acgagcgcgc gcccgcgcat cgagatgaac ctggagacct caatgagaaa
     5461 cgtaaccctt tcgttcctga tcgtcgcgct cgccgcggtg agcgcgtgca accgtacggc
     5521 cgaccagact cccgcgagcg gaccgcaggc gaaggcgccg agcacgccgc cgccgtcggg
     5581 cagcacgccg cccgcggagg tcaaccagcc gtcgagcggc gcgacccagc cgcaggcgcc
     5641 ttcggcgagc accggcacga gccacgcggc cgagtctccc tcgaaggagc tgagcaagca
     5701 ggaggagtcc accggccagc cgagcccggg caccggcggg gcagatcact cgacgccagc
     5761 gcgcgagccc ggcgccaagg gctgatccgc ccgggccggg aatgaggtag acggcttcag
     5821 cccgaagacg gcgcgccgct ggcgggttcc gcgcgagccg ccagctcgca gtacatgttc
     5881 gtgccgttgc actcgaagat cgtgaagcag ctggggcact gggcgatcat gccgatctcg
     5941 gccgcgcgct cgaagtcctc cagcgtcgtg aaggtcacgg gcgagcggaa gtcgcgctgg
     6001 cacctgacgc accggacgaa cgacgcggcg atcgatccca tgaaccactc cgcagaatcg
     6061 ccaccgctgg gacggcggcg gatatccgtg cgttggatcg aatagggccc ggctagttcc
     6121 tcgccggcag gcccggaagc ggatttatcc cgctgcgatg agaccgaggg acaccgcgcg
     6181 cacgaccgcg tgcggcgagc tcgcggtgcc cagcttccgg cgcgcgttgg tgaggtgaaa
     6241 tgtcacggtg cgctcggaga tctcgaggct ccggccgatc tcggcggcgg tctttccgcg
     6301 cgccgcgaga accaggcact cgcgctcgcg ccggctgagg gccacctcgg gcaccggctg
     6361 cgacaccggc accgacgcgt ggaggcgccg gcggatgatg cgcgcgcagg cgtcgtgcgc
     6421 gtacgccgcg agcagatgcg cctcggccat cgtgtcgagg atcgcgcgct cggcgtcgac
     6481 gccgccctgc gagctgatca tcgtcaaagc gctccactgg ccggccggcc cgtgcgtcgg
     6541 aaagatgacc ccggtggcga agccgaactc gcgcgccttg cgtacgagag tcggcgcctg
     6601 cggcatcagc tcgcgccaga gcacgggcgt ggtctgctgc tgggcacgcg cgcgcaccgg
     6661 gtcgctctcg gcgccgggac cgtgtttcag gtagtagcgg cgccatccgt tcgggcagtt
     6721 gtcgaaggag atctcgttga agcccgtgcg caggttcggg tagcgggcgc gcagggcgta
     6781 gtgggcgaag ccgagaccgg cggcggcgtg cgcgagctcg tctttcagat cggcgaacga
     6841 gccggccgtc atcacgcggc ggatccacga gctctcttgc gcgttcatgc gcgcctcttc
     6901 tttggcgccc gctgcagcat tcagccggcc gcctcccgga gcgtcactgt acgctttcgt
     6961 gtcagcgccg caagattctt tcggcaaaaa caacccgata gatcgcgtca ggggtttccg
     7021 gacagccggc gatcagtact gcgctgggca ccatgagtaa ttagacaata aaagcaaata
     7081 gatacgtttc ttgacttctc cccgttgtgc ggcgcaatat tcggtaccgt ttccgcggct
     7141 cttttgcgcc gcgctggagc ctagttgacc ggaaccgcag cattcgcagc cgaggagttc
     7201 taccgcgccg gccaggcgct gttccagaat tttttcggcg cgatggcggc acaatcgggt
     7261 ggcgggacgg gcgagccgct cgaggcctcg gcggcgaagc tcgcaagcct gcaggccgag
     7321 ttctcgcggc gtcacctggc gctgtggcaa tcggcgctgt cgcggggcgc ggacgccgag
     7381 cccgacgcgc ccgtcgaccg ccgcttcagc tcgccggagt ggcgctccgg tccctggttc
     7441 gactacgtgc gcagaagcta cgagatcaac gcgcgcttcc ttgccgattg cgtcgaggcg
     7501 atcgacgtcc agccgctcgc gaagcagcgg ctgcgcttcg cgacgcggca gatgatcgac
     7561 gcgctcagcc cggcgaactt cgtcgcgacc aatcccgagg cgatccggct cgcgctcgag
     7621 actggcggcg agagcctcac ccggggcgtg ctgaatctca tcgaggatgc gcacaagggg
     7681 cgcgtttcca ccaccgacga atcgcagttc gaggtgggcc ggaacctcgc cgtcacgccg
     7741 ggagcggtcg tgcacgagaa cgagctgatg cagctgatcc agtacgcgcc gcagaccgag
     7801 accgtgcacg agcggccgct cgtgatgatc cccccgtgca tcaacaagtt ctacatcctc
     7861 gatctgcagc ccgggaactc gttcgtgcgc catgccgtgg agcaggggca caccgtctac
     7921 atggtgtcgt ggcgcaacgt cgggcccgag cagggccgct acggctggga cgactacctc
     7981 gagctcggcg tgctgcgcgc gatcgacgtc gcgcgcgaca tcgcgggcgc ggacgcggtg
     8041 aacgcgctcg gcttctgcgt cggcggcacc atgctcggcg cggcgctcgc cgtgctcgcc
     8101 gcgcgcggcg agagcagggt ggcgagcgcg acgtatctcg cctccatgct cgacttctcc
     8161 gataccggcg acatcgggct cttcatcgac gaggcgagcc tcgccatgcg cgagacggcg
     8221 atcggccagg gcggcatcat gccggggcgc gagctcgcgt tcgtgttctc ggcgctgcgg
     8281 gcgaacgacc tcgtgtggtc gtacgtcgtc aacaactacc tcaagggcag gccgcccgag
     8341 gcgttcgatc tgctccattg gaacgccgac tcgaccaacc tgcccgggcc gatgtattgc
     8401 cagtacgtgc gccgcacgta tctcgggaac gagctgcgcg agcccgggcg cgtcagcatg
     8461 ctcggcagcc cgatcgatct cggcgcggtg aagacgccgg cgtacctgtt cgccacgcgc
     8521 gaggaccaca tcgtcccctg gcggacggcg taccgctcga ccgggctgct gggcggcgac
     8581 ctgcgcttcg tgctcggcgc gagcgggcac gtcgccggag tgatcaatcc cgctgcgaaa
     8641 aaccgccgca gccactggat cggtggcaat ttgcccccgg atccggatac atggttgcag
     8701 aacgcggtcg aacagcgagg aagctggtgg accgactgga gcgcgtggct cgctcctcac
     8761 ggcgggcgac gcgtgcaggc ccgcaaatct ctcgggagcg cgcggcacaa gcccatcgag
     8821 cccgcgcccg gccggtacgt caagcaccgc atcgcctgat caacggagac cgatatgact
     8881 gacgtagtga tcgttgcagc ggcgcgcacc gccatcggca agttcggcgg cgcgctttcg
     8941 aagattcccg cggccgacct cggcgcgcac gtgatcaagg cggttctcgc ccgcgcccgg
     9001 ctcaagcccg agcaggtctc ggaagtgatc atgggccaga tcctcaccgc gggcatcggc
     9061 atgaacggcg cgcgccaggc gtcgatccgc gccggcatcc ccgacatgtc accggcgatg
     9121 accatcaaca tggtgtgcgg ctccgggctg cgcgcggtgg cgctcgcggc gcaggcgatc
     9181 aaggcgggcg actcggagat cgtcgtcgcc ggcggccagg agagcatgag cctcgcgccg
     9241 cacgtgctga tgggctcgcg cgaaggcttc cgcatgggcg acgccaagct catcgacacg
     9301 atgatcaacg acggcctctg ggacgtgttc aaccagtacc acatgggcgt caccgccgag
     9361 aacgtggcga agcagtacca ggtctcgcgc gccgagcagg acgagttcgc ggcgaagtcg
     9421 cagcagaagg ccgaggccgc gcagaaggcg ggccgcttca aggacgagat cgccccggtc
     9481 gagatcgcgc agcgcaaggg cccggcgctg gccttcgacg ccgacgagta cccgaagcac
     9541 ggcacgaccg ccgaagcgct cgcggccctg aagcccgcgt tcgacaaggc cggcagcgtg
     9601 accccgggca acgcctcggg catcaacgac ggcgccgccg cggtggcggt catgtcggcg
     9661 cgcaccgcgg agaagctcgg cctgaaggcc ctcgcgcgga tcaaggccta cgcgaccgcg
     9721 ggcgtcgatc ccaagatcat gggcatgggc ccggtgccgg cgagcaagct gtgcctgtcc
     9781 cgtgccgatt ggaccatcaa ggacctcgat ctcatggaga tcaacgaggc cttcgccgcc
     9841 caggccatcg cggtgaacaa gcagatgggc tgggacactt cgaagatcaa cgtcaacggc
     9901 ggcgccatcg ccctcgggca tccgatcggc gcatcgggct gccgcatcct ggtgacgctg
     9961 atccatgaaa tgattcggcg cgacgccaaa aagggattgg cgtcgctctg catcggaggc
    10021 ggcatgggcg tagccctcgc cgtcgagcgc tgagccgcgg ggcgcaagca accgccgcgg
    10081 cgtatcacag ggagaagagg tcgatatgag ttcagtaata gcacccaaga cgcgcaaggg
    10141 cgcgagggca tccgacgagt cggacaagca gttgaaacag aggatcgcct tcgtcaccgg
    10201 cggcatgggc gggatcggca cggcagtgtg ccggcgtctc gcgaaaaacg gggtccgcgt
    10261 cgtcgcgggt tgtctcccgg ggtacgacaa gaaggagcag tggctcgacg cgatgcgcgc
    10321 cgaagggctg agcctctacg ccgccgaggg caacgtcgac gactacgagt cctgcgcgga
    10381 gatgttctac aagatcaaga gcatcgtcgg cccggtcgac atcctggtga acaacgccgg
    10441 catcacgcgc gacggcgtgt tcaagcgcat gtcgccgacc gactggtacg cggtgatcaa
    10501 caccaatctg aacagcgtgt tcaacgtgac ccgccaggtc atcgagggca tgatcgagcg
    10561 cggctggggc cgcatcgtca acatgtcgtc ggtgaacgcg ctcaaaggcc agttcgggca
    10621 gaccaactac tcggcggcga aggccggcat gcacggcttc accaaagcgc tcgcgcagga
    10681 ggtcgtgaag aagggcgtga cggtgaacac catctcgccg ggctacgtcg agaccgacat
    10741 ggtgaaggcg atgaagcccg aggtgctgaa gtccgtcatc gaccagattc ccatgggccg
    10801 gctggctcag cccgacgaga tcgcgggcct catcgggtac ctcgtgtcgg aggaggcggg
    10861 ctacgtcacc ggcgccaaca tctcgatcaa cggcggcctg cacatggagt gatcccggcc
    10921 gccgcgccag gggaagcccc gcgcgagcgg ggctttttga tttttgcagc gcaaatcgac
    10981 ttcgccgccg attcgcaaga gaatggagcg aaccgaagga ggagtcatgg cgcagcgatt
    11041 ggccctgatc acgggcggca tgggtggtct gggcgaaacc atcagcacga agatgtcgga
    11101 cgcgggctac cgcgtcgcgg tcacctattc gccctcgaac aagcaatccg agacctggat
    11161 cggcgagatg aaggcgcgcg gctacgcgtt ccgcgcgtac gcgtgcgacg tcgccgacta
    11221 cgattcggcg aagcgctgcg tggaggcggt cgtcgccgac gtcgggccgg tcgacatcct
    11281 ggtgaacaac gccggcatca cgcgcgacat gacgttccgc aagatgggca aggccgactg
    11341 ggacgcggtg ctgcgcacga acctggactc ggtgttcaac atgaccaagc aggtgtgcga
    11401 cggcatggtg gaccgcggct ggggccgcgt catcaacgtc tcgtcggtga acggctcgaa
    11461 gggcgcgttc ggccagacca actacgccgc cgcgaaggcc ggcatgcacg gcttcacgaa
    11521 gtcgctcgcg ctcgaggtcg cgaagaaagg cgtcaccgtg aacaccatct cgcccggcta
    11581 catcggcacg aagatggtga ccgcgatccc gaaggagatc ctcgactcga agatcctgcc
    11641 gcagatcccc gtgggccggc tcggcaagcc cgacgaggtc gccgggctca tcatctacct
    11701 ctgctcggag gaggcggcct tcgtcaacgg cgccaacatc gccatcaatg gcggccagca
    11761 catggcctag gcgcggcgtc ccggcgcctg cggctcgccg acgcgcccca ggttcgtcgc
    11821 cgccgcgcgg atcgtcgcct gcgacaggtc ggcgaactgc tgggtcgccc ggttgaggtt
    11881 ctccatcacc gccgcggacg cggcgatcga cgacctcacg gcggccaccg cgacttcggc
    11941 gcccggagtc gcgctctcgt ccgcgcgccg cgcgagatcg tcgttcaggc ccgagagctg
    12001 ctgctcggcg tgctgcgcaa gctgcgcctg cgtctcgcgc acgagctcgt agacttcctg
    12061 ggcgtacgac gccgcctgct ccatggtctg ctgcgcgaga cgcgagcgca gccgcatgag
    12121 ctcggccggg tcgctcgtgc cgagcagggc gcgcacggcc tgcgcgtgct gctcgagcgc
    12181 gcggcgcgcg gcgtcgatgt tcaggcgcag cagctgctcg gctcccgcga gcgccgtctg
    12241 ggcgcacgcg atggtggtat cgatggcggc tcgctgccac cccgagagcg tctccggtgc
    12301 atgggccatg gcactcctcc tggcaaaagg ggtctgcttt gaagatagac cgctttggtg
    12361 cagaatacat tcaatattgc ggagcagcat cgagcttagt cgtcaaagcg ccgaatccag
    12421 agagaaaccg atgtcgcagc gcaacgcggc cagcgagggc cccatggcgg aacccgtccg
    12481 actgatcaag aaatatccca accgacgtct ctacgacacc cggacgagca cctacatcac
    12541 gctcgcggac gtgaagcagc tggtgctgca gaacgaggag ttccaggtcg tcgacgcgaa
    12601 gacgagcgag gatctcacgc gcgcgatcct gctgcagatc atcctcgagg aggaggcggg
    12661 gggcgccccg atgttctcct ccgacgtcct gacgcagttc atccgctcct acggcaatgc
    12721 gatgcagggc atgctgcgcg gctacctcga gcgcaacatg cagttcttca ccgagatcca
    12781 gaagcgcatg caggagcagt cgaagcagct ctacggcgag ccggtgaagt tcaacgagga
    12841 cctgtggaag cagttcatga gcttccaggc gccggcgatg cagagcctca tgtccgcgta
    12901 catggagcag agccgcaaca tgttcctgca gatgcaggag cagctgcaga accagacgcg
    12961 caacatgttc tccgggttca cgttcccggg ctttcccgga ttcggcgcgg ccgccggcgg
    13021 cgctgcgaaa gccgagggcg aggaaaagaa gcccgacgac gagaagaaat cctgatcttc
    13081 cgcattcgca acgccccgtg aacgccgacc tgctatcctt gtggcaggtc tcccgaagcc
    13141 gttcgatcag cagtgtctaa ctggcgcgtc gttcatgcgt aagccgccga gaatcgcagt
    13201 gtttctcgcc gttctcgcgg cgctcggcgc atgggtggtc gtcgaccagc gctcgggccc
    13261 gcgcgcgccc ggacccgcgt ccggcggaca gcccgcgggc gcggcacgcg cgccttccga
    13321 gttccgcgcc gagctgcccg agcgagcgcc gctgcagcgt gccgggaagg acccgttctc
    13381 gacgcgatcg tggacgccgc cgcccgcgcg ccggcccgcg ggccccgtcg ccgccgcgcc
    13441 gccgccggtc gcgccgccgc tgccttatcg ctacgtcggc gagctggtgc tcccgacggg
    13501 cacgcaggtc tatctcgcgc gcggcgacga tactttccgg gtgagcgaag gcgagacgct
    13561 ggacggccaa taccgcgtgg aatccatcaa gcgcggcgag atggtgttcg tgcatctcgc
    13621 gaccggacag cagcagacga tgcgcttcag cctgcccggc gaggacgccg aagcgcaggc
    13681 gcgcgcgatc gtgcgcagcg cggacggcgc gccgcgtccc gccggagcgc cggccgcggt
    13741 tctgcccgcg agctccccga cggcaccggc cactgcgagc gccggtccgg ccgagcccgc
    13801 gcgcctgaga tgggacggtc ccgcgagcgc gaaagccggc gcgaccttca acgtcgcgct
    13861 gcgcatgacc tcggcgagcg agcagatccg ttccgcgccg atggaagtgc gcttcgaccc
    13921 gaagctcctg gagccggtct cggtgcgccc ggggcgctat ttcggtgcgg accgctccaa
    13981 cttcggctat cgcatcagcg gggaaggctc gatctacatc ggcgtgtcga accggacgcc
    14041 gccgtccacg gcgaacgacg cggagctcct cgtgctgacc ttcaagccga tcaaggcggg
    14101 cgctctggcg gagctgaacg tcgcggcgct caatctgcag gggaccgcgg gtcgtccggt
    14161 cgcatacacg gcgctgggcg cgttcaagac cacgatcatt ccctgatccc cacccgaccc
    14221 cgctccgccg tagtatcctt cgatcaataa agggaaagca tatttcccgg ggagggcagg
    14281 tggccgctcg tcagcgttct cgccgcagct tctcgtcgcc tgatcgggcc tgcgcctgat
    14341 gtcggcgatc cccgccaacg caccggccgc gggccagcgc accgtccgcc ccgagttcac
    14401 cgcctcgggc gccgagtact tccgcatctg gatcgtcaat ctcctcttca cgctgctcac
    14461 cgcgggcatc tactcggcct gggcgaaggt gcgcaagaag cgctacttct acggcagcac
    14521 gaagttcgac ggctcgacgt tcgattattt cgccagcccg cgcgcgatcc tgaacggccg
    14581 catcatcgcg ttcgtggtgt tcgtggtgta cgcgatcgcg ggggacctcc atccgcagtc
    14641 gcacgtcgcc tttctcgcgg tgggcgggct ggcgctgccg tggctggtgg tgcgcgcgct
    14701 cggcttcaac gcgcgcaatt cggcatggcg cgggctgcgc ttcgatttca cggcgacgac
    14761 gcgccaggcg atcacggtct acatcggcta tttcctgatc gtcgtgctga cgctcggctt
    14821 cgcgtacccg tggttcgtcg cacgccagaa ggcgttcgtg atcggcaacc acgcgctcgg
    14881 gaaatcgcgc ttcggctgcg acatgccggc gcgcgcgctc ttcggcatct acgtccgcgc
    14941 cgggctgctc atgacggtgc tcgtcgcgcc gctggtcggc ttgatgacct tcgtgatggt
    15001 gaagatgccg gtccccgaga atctcgcctg gctcaagttc acgctgccga tcctgccgat
    15061 gtacgcggcc tacgtcgcgg tctacgccta ttcgcaggcg cgcacgcaga acctgatgtg
    15121 gaacagcgcg tacggccccg ggatgcgctt ctcgagcacg ctctcggcgg tgcgcctcgc
    15181 gcggatctac atcggcaaca tcgtggttgc ggcgtgcagc gccgggctgc tcatcccctg
    15241 ggcggtggtg aggacgatgc gctaccggct cgagcagttc gccgtcgtgc tcgagggcga
    15301 cccggtgcac gaggcgagcc cggcgctcgc gcgggtgggc gcgaccggcc aggagctcgg
    15361 cgacttcttc aacctggatc tcggcctgtg aacgtcatcg ccggcgacta ctacgacggc
    15421 cgcacctcgg tgcggcacgc ggtgagcgcg atcgtgtcga acggccggct gaaggtcgtc
    15481 ggccgcgacg tgaacctgga gctcgacctg cgccgggtgc ggcgttcgct gcgcgtcgcg
    15541 aacacgccgc gctggctcta ccttccgggc ggcggcgcgc tcgtcaccgc ggacaacgac
    15601 gcgatcgacc gcatgacgcg caagcgccgc tacgaccggc tgttgcaccg ctgggagtcg
    15661 cgccccgcgc tggcggcgct cgcgatggtc gcggtgatcg cgtcgctctg gctcgcgttc
    15721 gaccgcggcg tgccgctggc ggtggagcgc atcgccgagc gcatcccgcc gaccgcggag
    15781 gcggcgctgg gcgagcagac gctgcgcagc ctcgaccagc ggctgacgca gccgtcgacc
    15841 ctctcgcgcg agcgccaggc ggcgctgcgc gagcgcttca cggccatgac gcaggcggct
    15901 ggcgacccga cgccttaccg cctcgagttc cgctcgagca agttcatcgg ggcgaacgcg
    15961 ttcgcgctgc cctcgggcat catcgtgatg acggacgagc tggtgaagct ctcgcgccgc
    16021 gacgaggagg tgctcgcggt gctcgcgcac gagctcgggc acgtccacca ccggcatacg
    16081 atgcggcgcc tgcttgaggc gtccgccacg ggactcatca tcgccgccgt caccggcgac
    16141 gtcgcctcgg ccacgtcgct cgcggccgcc gcgccggcgg tgctgctcca gacgcggtac
    16201 tcgcgcgaca acgagcgcga ggcggatgcc tattccatcg ccatgctgca gaaatccggg
    16261 ctcagcccgc gcaacatggc gaccatcctc gggcgcctgg aggcgaagca cgccgggcgc
    16321 cgccgcggcg ggctccccga cttcctgtcc tcgcacccgg cgacgaaaga gcgccaggcg
    16381 caggcgctcg ccgccgcgcg cgacgacgcg ccggccgacg acgcgacgcc cgccgacggc
    16441 gccgtgccga tgccggcgcg cgccccgcgc ggagtcatcg tcgacccggt gcagcgccag
    16501 gtcgtcgagc tggtcgagaa gcgcgactac gagacgctcg agcggctgct ctcggagcgg
    16561 caggccgcgt tcgagcgcga cccggcgacg agcccggtgc tcgacagctc gtaccgcgcc
    16621 ttcgacaagg tggacccggc gtccgcgccc gcgctcgacg aatggattgc gcgctatccg
    16681 agctcgtacg ccgcgcgcgc gggccgcgcg accttcctgc tctaccgcgg acaggacgcg
    16741 cgcggcaccg ccacgttcga cgagaccccg cccgagaaca tcgaggcgat gcgtgcgctc
    16801 ctcacccgtg cgctcgagga cgcgggccgg tcgctcgcga tgacgcccaa gccctacatc
    16861 agccaccgga cgctgatgac cgtcgcgcgc ctgtcggggc ggcgcaagga agcgctcgag
    16921 cactacgagg cggcggtgcg cctggcgccc gagagcatcg acacgcgcct ggatcacatg
    16981 acgctgctcg agccgcgttg gggcggcagc tacgcggaga tggagaagtt cgccgcccgg
    17041 tcgcgcgcgg agctgaaagc cccgggcggc gccgaccggc tcgccgcgca cgtcggcgaa
    17101 taccgggccg acgagagcct gcgcgcgcgg gacttcgcgg cggcgctgcg cgactacgac
    17161 tgggcgctcg ggctcgatcc cggcgccgcc cggtcgtgcg gaagggccta cgcgctgagc
    17221 gagctcaagc gcgacgacga ggcgctcgcc gacgtcaagc gcgttctcgc gatagcgccc
    17281 gaccagcgct actgccgcga gctcgctcct catgtcgcct cgcgcgccgc gaacgcgggc
    17341 gaggcgatcg cggtgatgaa cctggtcatc gacgccgacc cgagctcgac gccggcgctg
    17401 acccagcgcg gctggcgcta ccagcagcag aaccggcccg agctcgcgtt cccggactac
    17461 ctcgccgcgg cgaagctcgg cgacccctgg gccgagctcc aggcgggcaa gttcctgtgg
    17521 aacggcctgg gcgtcaaggc cgaccgggaa gaggccctcg gctggctgcg caaggccgcc
    17581 gcgcacggcg accgcgacgc caaagtcagc ctcgagcagg ccctcgcgga gctcgcgcgc
    17641 aagtaaggtt cgggcgcggg tttttgtgta atctaggcgg cgcttcgctc tccccctaaa
    17701 tcagaaattc ctgaaggagg actcccccat gcgttggtta cgtcttctcc tgttgtccct
    17761 ggcacttgct cccctggctg cttccgcgtg ggatcgcggc gacgtccagc ggttcgcgac
    17821 gcttccttcg ggtgcagggc atcccgaggg tatcgccgcc gacaaggacg gaaacatcta
    17881 cacgacgatc ttcgatccca ccggcgcggc gaccaataag caccagatgt tcgtcttcga
    17941 ccgccagggg cgtctggtgc gcgaggtgaa tatcgccggc gccagccacg cgttgctcgg
    18001 gcttgatttc caccccacca cgggcgcgct actcgtgctc gacttcgggc agggcaaggt
    18061 gctcaaggtc gatcccgtga ccggcgcgtc cacggttttt actacggtca ccggggcttc
    18121 cggcctgaac gcgctgacct tcgaccagca gggcaacgtc tatatctccg attccttcca
    18181 gggcatcatc tggaggaccg ggccgaacgg cagcccgacg gcgacggtct gggcgcagga
    18241 cccgctgctg cagccgaacg ggattccgcc tttcggcgcg aacgggctcg ggttcaacaa
    18301 ggcgttcaat acgctgttcg tcgccaatac cgcgaacgac tcgatcatcc agattcccgt
    18361 gacgccaagc gcgacgggcc ccgtggcggg tccggcgtcc gcgctggcct acagcgtcaa
    18421 cggcgccgac ggactgatcc tcgacgctga cgacaatctc tggatctgcg cgaaccagtc
    18481 ggacgagatc gtcgtgctcg acaagaccgg gaaggtgatc agcaagctcg gcgacttcga
    18541 cggcgtcgac cgcgaccagg cgccgatcgg gctgctgttc cccgcgagcc cggtgcgcgt
    18601 gggcgactgg atctacatca ccaacctgtc gctcgatctg cgcaacgtcg gtggaccgca
    18661 gacgatcgac agccaatggg ccgacgaggt gcagacgcag acgctcgccc gcatccgcgc
    18721 ccgcatccgg ccgaaccgcg agtgagccag gccaccgcgg agaaaaggcg ccttcgggcg
    18781 cctttttttc gccgacgcgt ccggtcaggg ggagccttac gccgcggcga ttgattgtcg
    18841 aatcggcttt tcggaccgcg cacgaccacc cggcgcaccc gcactgaccg ccgctcagcg
    18901 cgcgggcggc ggcgccggca tcggcgcgat cggctgctgc gggttggccg cgcccggcgc
    18961 gggcgcgccc
//
LOCUS       DATDMW010000139        13083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_912788, whole genome shotgun sequence.
ACCESSION   DATDMW010000139 DATDMW010000000
VERSION     DATDMW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912788"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1048
                     /locus_tag="VI319_06060"
     CDS             53..1048
                     /locus_tag="VI319_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY6863446.1"
                     /translation="MSITFEVVVFLIAVALVFDFLNGFHDAANSIATIVSTGAMSPRW
                     AVAWAAFFNFVAFLVFGAAVASTVGKGIIDISIVDHRVVFGALVGAIAWNLITWYYGI
                     PSSSSHALVGGLCGAAIAKGGVHTLVPAGVLKTAVAIVLSPAGGFVLAVALMIAVSWI
                     FHRSTPKRVDRWFRYLQFVSAAFYSIGHGGNDAQKTMGIIWMLLIASGHGSSDHLPFW
                     VVLVCQAAMGLGTLFGGWRIVKTMGMRITKLKPVGGFCASASGAITLFIATGLGIPVS
                     TTHTITGSIVGVGSARSFNAVRWGVAGNIVWAWLFTIPGSAFIAGIAWWLGTQYF"
     gene            1048..2118
                     /locus_tag="VI319_06065"
     CDS             1048..2118
                     /locus_tag="VI319_06065"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY6863447.1"
                     /translation="MQPLRASSLARYLFGAYVLLVIYASLHPFSGWQVQGPTPFAFLT
                     APFPRAITRFDVVTNVLGYVPLGFLALLAVQPRLGGRRALAFGIGCSILLSFVLESLQ
                     SYLPSRTASNVDLLANAAGGTLGALLAMPAAPRLLADGGLKALRYRLIAAGAEFDLGL
                     VLIALWLLAQLSPETLLFGTGDLRELFQPPPGKLYPAELFVRVEAGVAAANTIAVGLF
                     VSCLTAARQPARLLFGALLGAALALRWIAFDVLMRDMLWITSGAVAGLAAGVLVVLAG
                     VSLPRTARLAVAGLALMGATALVNLAPGNPYLAASLALWQRGAYFNFNGLTHVVSVTW
                     PFAAMFYLVVLAAERGRDRGTI"
     gene            complement(2115..2603)
                     /locus_tag="VI319_06070"
     CDS             complement(2115..2603)
                     /locus_tag="VI319_06070"
                     /inference="COORDINATES: protein motif:HMM:NF045536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070088 - polyhydroxyalkanoate granule
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate granule-associated phasin"
                     /protein_id="HEY6863448.1"
                     /translation="MRTPSFTTLYGDPFAAWTRLAWKTGELMLHSGEVIGRRVTGFAL
                     AGPGDGREIALMSREKVEAAAESGYAMWWSLVRINQQLAGLALGQMFAGAAGMASLAS
                     GATPAQAVERQARLVRDAVGRSATAAAAISGSTARLAQRGLTPIRSRAARNAKRLRKR
                     RR"
     gene            complement(2600..4192)
                     /locus_tag="VI319_06075"
     CDS             complement(2600..4192)
                     /locus_tag="VI319_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY6863449.1"
                     /translation="MPEYSLSDYHERSKHRLDRYAPGPRGLDWASQPDPFRRYEGCAL
                     VELPLAAEALEVRWADIRAARPPAARPLDLGSIALLFELSLAVSAWKSYGGTSWALRC
                     NPSSGNLHPTEGYLVAGAIPGIEAGVYHYASREHALERRGAWQDAPALGGVLVGLASI
                     HWREAWKYGMRAYRYCQHDCGHALAALAYAAACLGWRARLLEEPGDAEVSALLGLDRD
                     GDYAGAEREVPEALLWIAPADRDAPALPHPPERWAGRANRLSQGHVAWPDIDAVERLA
                     TKPRTVPERPVRTPAAPAPVRGDASAAAVIRGRRSAVAFDGATSITAEAFFAMLDATL
                     PRAAPPWSAWPGPALVHPAIFVHRVEGLEPGLYLLVREPAALDALRAAMRRDWLWRKT
                     GPVGLPLHLLLAHDLRGAAQSVSCHQEIAADSCFALGMLARFGAARAEPWRYRALHWE
                     CGMLGQVLYLEAEAAGIRGTGIGCFFDDEMHGLVGLRDTEWQTLYHFTAGGAVEDTRL
                     TTLPAYMHRRHELARAGTVIPS"
     gene            4234..4542
                     /locus_tag="VI319_06080"
     CDS             4234..4542
                     /locus_tag="VI319_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY6863450.1"
                     /translation="MSYYDKHVFFCCNQRTNGEACCNDKGASAMRDYAKKRVKELGLA
                     GKGAVRINQAGCLDRCEEGPCIVVYPDEVWYTYVDRADIDEIIEEHLKNGRVVERLRL
                     "
     gene            4539..5156
                     /locus_tag="VI319_06085"
     CDS             4539..5156
                     /locus_tag="VI319_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6863451.1"
                     /translation="MSAPAERVFVAGDAGRIETVIDLPAASGSGAPRGLALVAHPHPL
                     YGGTLDNKVAQTLARAFVELGYVALRPNFRGVGQSEGEHDEGDGETDDLVKVAQYASG
                     RFGALPVALAGFSFGAFVQTRVAKRIVPERMALVGLAVRRAGTDTVPADTVLIHGERD
                     DTVPLADVLRWAEPQELPVVVIAGADHFFHRRLHVIRRIVQDAWR"
     gene            5227..5640
                     /locus_tag="VI319_06090"
     CDS             5227..5640
                     /locus_tag="VI319_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY6863452.1"
                     /translation="MLWVKTLHIIFVVTWFAGLFYLPRLYVYHAMTDDRPGRERFKIM
                     ERKLYWGITTPGGVLAIVFGVWLWLGWGFGGGWLYAKLAVVALLVAYHLWCGKLLVDF
                     REDRNTRSHVWYRWFNEAPTLLLLAGVALAVVKPF"
     gene            5646..6860
                     /locus_tag="VI319_06095"
     CDS             5646..6860
                     /locus_tag="VI319_06095"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY6863453.1"
                     /translation="MPASVTPESIRQAAAVIAGSVEKTPCFESRALSEATGARVWLKF
                     ESHQFTSSFKERGALNKLSSLSAAERSAGVLAISAGNHAQGVAYHAQRLGIRAVIVMP
                     RLTPHVKVQRTRRFGAEVILIGDTFDEAKTAGLALAAERSLVLIHPYDDEHVISGQGT
                     VALEMLAAQPALDTLIVPVGGGGLIAGIAVAAKGINPGIEVLGVETESYPAVHCALRG
                     VPPAFAHTIAEGIALKEPGTRTLPIIREKVSDVVLVSESEIEQAIVMLLEAEKAVVEG
                     AGAAPLAALVRYGERFRGRRTGLVVSGGNIEPLLLSEIITRGLVKAGRLARLRVRVRD
                     LPGSLARVTACLAAADANIEEVHHQRAFAADLPLQQVEIEFVLQTRGPEHVQELIAAL
                     DRSGFPARLTLD"
     gene            7044..7556
                     /locus_tag="VI319_06100"
     CDS             7044..7556
                     /locus_tag="VI319_06100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6863454.1"
                     /translation="MTAKNITLRPGRPDDAEKIAMMSRDLIETGLGWSWGPDRVSRSL
                     RNKDTLTVIACDRERIVAFAIMYFGEEHAHLNLLAVRPSHQRLGIGRRLVEWMVESAF
                     SAGIATIHLELRASNYTARSFYRALGFAESAYIPGYYRGREMALRMMRVLRKPGMPLP
                     AWPIPKLPKS"
     gene            7526..10183
                     /locus_tag="VI319_06105"
     CDS             7526..10183
                     /locus_tag="VI319_06105"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY6863455.1"
                     /translation="MADPEAAQELIPSRRRAAPALLLVLSLVAPGVFAPEAVAAPPVG
                     PGISRALAAWRAPRYRDVRYSLDLEVDRDAARLAGTVQIELTLASPPPDVVLDWRPAA
                     GTRLEDLRVDGAPAAAARVVRDHIVVPARLLHPGRNTVRARIEAPIAVAGGALTRYED
                     HEDGSRYVYTLFVPADASTVFPCFDQPDLKGRFRLELAAPASWTVIGNGPARDVEDRG
                     ATKRWRFEETPPISTYLFAFAAGPFVEAGNGTDLRTRLFVRRSRAERASREAGDVLEQ
                     NARALDWLAEYFDRPFPFAKYDLVLVPEFAYGGMEHAGAGFLREESVLFPSEPNENDR
                     LRRAQLLFHEATHQWFGDLVTMKWFDDLWLKEGFANFMAAKATEALLPKDPAWTAFHA
                     LKVTAYRTDVTRGTTPIFQPLANLAAAKSAYGTIVYGKAPAVLRQAEFYVGEAAFRAA
                     VRAFVAAHAYGSADWSDLVHMLERASRKDLRAWAAAWVQRRGMPRVRLAWSTDASGAV
                     RGATLRQSDVLREGGVWPMRVRLAAVAPDGSARGFDATLRGRRAPLPALDGIPAPALL
                     FANDGDYGYGQFLLDAKSRATVLARPDLARDALLRSLVFDAVWDSVREAELAPREYID
                     FALRAAPAERDPVTLSTLLARVEAAFRVYLSDAQRDAVAPALERTLVNGMLGADSAGR
                     RISFFRAFADSAWSPEGRAVIKRLLARALEVPGVPLSSRDRFRLVARLLVLGDPDGET
                     LLAAQAEADPGDDGRRYAYAAGAARRDAAVKSRYFERALRDAALPESWVEAALGAQNA
                     PEHAALTAALVEPALRALPELKRSRKIFFVNEWLSAFLGGQRDAEVLAVVRRFLQRPE
                     LDRDLRLKVLEAVDPLERAVRIRAKFAAG"
     gene            10180..11340
                     /locus_tag="VI319_06110"
     CDS             10180..11340
                     /locus_tag="VI319_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEY6863456.1"
                     /translation="MTDRFDLFATCPRGLEALLAEELGALGAREPRTVPGGVAFRGDL
                     AACYSANLGSRFATRVLLRLGTAPYRREQDVYDAALALPWPEWFGERQTIRVDLNATR
                     SPLKSLDFATLRVKDAVCDRFRADRGSRPDVDTRSPDVRIHCYLDAKQAMFYLDTSGE
                     PLYKRGYRKDTGEAPLRENLAAGIVALTGWDGVEPLLDPMCGTGTLVIEAALKALAIP
                     PGHARGFGFERLRVHDAKQWERVRDGMLAKRQSRPLAVFGRDRYGDELKKAERNVEAA
                     GLSGSIELKQADVLESSAPAKSGLLVANPPYGVRLGDKNELAAFYPRLGDALKRHYAG
                     WRCFVFSGDPQLAKLIRLHPSRKIPLYNGALECRLYEYRMVAGSMRKDARPR"
     gene            complement(11337..12077)
                     /locus_tag="VI319_06115"
     CDS             complement(11337..12077)
                     /locus_tag="VI319_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY6863457.1"
                     /translation="MPVSWESLAVAAAVVACGYFVFGLSGFGAALVTVPVVSHLWPVQ
                     FVLPVLALLDFTASMFLGVRHHREAERGELTRMIPMAVLGAALGATLLVNIPRGAGLA
                     GIGAFAVLYGFYALVERGAFGRISRNWAYVAGIVGGTSGALFGIGGPPYAIYLSRRIE
                     DKDVLRATVATMVIFSVGSRLVMFAIAGLVAWNELKTALVLLPFLFAGLWAGGRAHLR
                     ISRRALGRFIAALLIATGASLLWRAAAG"
     gene            complement(12082..12513)
                     /locus_tag="VI319_06120"
     CDS             complement(12082..12513)
                     /locus_tag="VI319_06120"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY6863458.1"
                     /translation="MVQHPDISHLISLAVAPVFLLTAVGTLLGVLSNRLGRVVDRIRM
                     LDELAPSLDVKARAACDSELAVLSRRMRLVYTAMTAAVLCGLFVGLLIIVAFVDALVA
                     VDLWHTVGLLFIAAMLSFIGSLCVFLREIFVAVTSTHSPMR"
     gene            complement(12517..12849)
                     /locus_tag="VI319_06125"
     CDS             complement(12517..12849)
                     /locus_tag="VI319_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863459.1"
                     /translation="MAEPSHTYPELKSKNLAELREIAAGIQHDAVQGATQMNKDHLLA
                     AICKALNIDMHVHHEVKGIDKAAVKGKIREWQKKRDEALKAKDRKQLKVALDHIHHFK
                     HALRKAMA"
     gene            complement(12939..>13083)
                     /locus_tag="VI319_06130"
     CDS             complement(12939..>13083)
                     /locus_tag="VI319_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HEY6863460.1"
                     /translation="YEAGKISYEDALRNADSVNDLRLAIKLQSKEAKNRDLTEGIQNL
                     NIV"
BASE COUNT         1797 a         4727 c         4600 g         1959 t
ORIGIN      
        1 gcgaggacgt ggcgaacatc atcgaaggca tcgtgctcga gaacgcctga gcatgtcgat
       61 cacgttcgag gtcgtcgtct tcctgatcgc cgtcgcgctc gtattcgact tcctcaacgg
      121 cttccacgac gcggcgaact cgatcgcgac catcgtctcg accggcgcga tgagtccgcg
      181 ctgggcggtg gcctgggcgg cgttcttcaa cttcgtcgcc ttcctcgtgt tcggcgcggc
      241 cgtcgcgtcg accgtgggca agggcatcat cgacatctcc atcgtggacc accgcgtcgt
      301 gttcggcgcg ctcgtcggcg cgatcgcgtg gaacctgatc acctggtact acggcatccc
      361 gtcctcgtcc tcgcacgcgc tcgtcggcgg gctgtgcggc gcggcgatcg ccaagggcgg
      421 cgtccacacg ctggttccgg cgggcgtgct gaagaccgcg gtcgccatcg tgctctcccc
      481 cgcgggggga ttcgtgctgg ccgtggcgct gatgatcgcc gtgtcctgga tcttccaccg
      541 ctccacgccg aagcgcgtcg accgctggtt tcgctatctg cagttcgtct cggccgcctt
      601 ctacagcatc ggccacggcg gcaacgatgc gcagaagacg atgggcatca tctggatgct
      661 gctgatcgcg tcggggcacg gcagctccga tcacctgccc ttctgggtcg tgctcgtgtg
      721 ccaggcggcg atggggctcg ggacgctgtt cggcggctgg cgtatcgtca agaccatggg
      781 catgcgcatc accaagctca agcccgtcgg cggcttctgc gcctcggcga gcggggccat
      841 cacgctgttc atcgcaaccg gcctcgggat cccggtgtcg acgacgcaca cgatcaccgg
      901 ctcgatcgtc ggcgtggggt ccgcgcgcag cttcaacgcc gtgcgctggg gcgtggccgg
      961 caacatcgtc tgggcgtggc tcttcaccat cccgggctcg gcgttcatcg ccggcatcgc
     1021 gtggtggctg ggaacgcagt acttctgatg cagccgctgc gcgcatcgtc gctcgcgcgc
     1081 tatctcttcg gcgcctacgt tctgctggtg atctacgcga gcctgcaccc gttctccggc
     1141 tggcaggtgc aggggccgac gcccttcgcg ttcctgaccg cgcccttccc gcgcgcaatc
     1201 acccgcttcg acgtcgtcac caacgtgctc ggctacgtgc cgctcggatt cctcgcgctg
     1261 ctcgcggtcc agccgcggct gggcggacgg cgcgcgctcg ccttcggcat cggctgctcg
     1321 atcctgctca gcttcgtgct cgagtcgctg cagagctatc ttccgagccg caccgcgtcg
     1381 aacgtcgacc tgctcgcgaa cgccgccggc gggacgctcg gcgcgctgct cgcgatgccc
     1441 gcggcgccgc ggctgctcgc ggacggcggc ctgaaggcgc tgcgctaccg gctgatcgcc
     1501 gcgggcgccg agttcgacct cgggctggtc ctgatcgccc tctggctgct cgcgcagctc
     1561 tcgcccgaga cgctgctctt cgggacgggc gacctgcggg agctcttcca gcccccgccc
     1621 ggcaagctct acccggcgga gctgttcgtc agggtcgagg ccggcgtcgc cgcggcgaac
     1681 acgatcgcgg tgggcctgtt cgtctcctgc ctcaccgcgg cgcggcagcc ggcgcggctc
     1741 ctcttcggcg cgctcctcgg cgcggcgctc gcgctgcgct ggatcgcgtt cgacgtgctg
     1801 atgcgcgaca tgctgtggat cacctcgggc gcggtggccg ggctcgccgc cggcgtgctg
     1861 gtcgtcctcg ccggcgtgtc gctgccgcgc acggcgcgcc tcgcggtcgc cgggctcgcg
     1921 ctcatgggcg cgaccgcgct cgtcaacctc gcgccgggca acccgtacct ggcggcgtct
     1981 ctcgcgctgt ggcagcgcgg cgcctacttc aacttcaacg ggctgaccca cgtcgtctcg
     2041 gtgacgtggc ccttcgccgc gatgttctac ctcgtggtcc tcgccgccga gcgcggccgc
     2101 gatcgcggaa cgatctagcg gcgtcgcttg cgaagccgct tcgcgttccg ggcggcgcgg
     2161 gagcggatcg gcgtgagccc gcgctgtgcg agccgggcgg tcgagccgga gattgcggcg
     2221 gccgccgtcg ccgaccggcc gacggcgtcg cgcaccagcc gcgcctggcg ctcgaccgcc
     2281 tgcgccggcg tcgcgccgga ggcgagggaa gccatccccg ccgcgccggc gaacatctgc
     2341 ccgagcgcga gccccgcgag ctgctgattg atgcgcacca gcgaccacca catcgcgtag
     2401 cccgattccg ccgccgcctc caccttctcg cggctcatga gcgcgatctc gcggccgtcg
     2461 ccgggtccgg cgagcgcgaa tcccgtcacg cggcggccga tgacctcgcc ggagtgcaac
     2521 atcagctcgc cggtcttcca ggcgagccgg gtccaggcgg cgaacggatc gccgtacaaa
     2581 gtcgtgaacg atggggttct catgagggta tgacggtgcc ggcccgcgca agttcgtgac
     2641 gccgatgcat atacgcgggc agcgtcgtca gccgcgtgtc ctccaccgcg ccgcccgcgg
     2701 tgaagtggta gagggtctgc cactccgtgt cgcgaaggcc gaccagaccg tgcatctcgt
     2761 cgtcgaagaa gcagccgatg cccgtgccgc ggatgccggc cgcctcggct tcgagataga
     2821 gcacctggcc gagcatcccg cattcccagt gcagcgcgcg gtagcgccac ggctcggcgc
     2881 gcgccgcgcc gaagcgcgcg agcatgccga gcgcgaagca cgaatcggcg gcgatctcct
     2941 ggtggcagga cacgctctgc gccgccccgc gcaggtcgtg cgcgagcagc aggtggagcg
     3001 ggagccccac cggccccgtc ttgcgccaga gccagtcccg ccgcatcgcc gcgcgcagcg
     3061 cgtcgagggc ggccggctcg cgcacgagca ggtagaggcc gggctcgaga ccttcgacgc
     3121 ggtgaacgaa gatcgccgga tgcacgagcg ccggccccgg ccaggccgac cacggaggcg
     3181 cggcgcgcgg cagcgtcgca tcgagcatgg cgaagaacgc ctcggccgtg atcgaagtgg
     3241 cgccgtcgaa ggcgaccgcg ctgcgccggc cgcggatgac cgccgcggcg ctcgcgtcgc
     3301 cgcgcacggg cgccggcgcc gcgggcgttc gcaccgggcg ctccggcacg gtgcgcggct
     3361 tcgtcgccag gcgctcgacg gcgtcgatgt ccggccaggc gacgtgacct tgcgacaggc
     3421 ggttcgcgcg gcccgcccag cgctcgggcg ggtgcggtag cgccggcgcg tcgcggtccg
     3481 cgggcgcgat ccacagcagg gcctcgggca cctcgcgctc ggcaccggca tagtcgccgt
     3541 cgcgatcgag gccgagcagc gcggagacct cggcgtcgcc cggctcctcg agcagcctcg
     3601 cgcgccatcc caggcacgcc gccgcgtacg cgagcgccgc gagcgcatgc ccgcagtcgt
     3661 gctggcaata gcggtacgcg cgcatcccgt acttccacgc ctcgcgccag tggatgctcg
     3721 cgaggccgac gaggacgccc ccgagcgcgg gcgcgtcctg ccacgcgccg cggcgctcga
     3781 gcgcgtgctc gcggctcgca tagtggtaca cgcccgcctc gatccccggg atcgcgcccg
     3841 cgacgagata gccctcggtg ggatgcaggt tgccgctcga gggattgcag cgcaacgccc
     3901 acgaggtgcc gccgtagctc ttccacgcgg acacggcgag cgagagctcg aacagcagcg
     3961 cgatcgatcc cagatcgagg ggccgcgccg cgggcggccg ggcggcgcgg atgtccgccc
     4021 agcgcacctc gagcgcttcc gccgcgagcg gcagctcgac gagcgcgcag ccttcgtacc
     4081 ggcggaaagg gtcgggctgg ctcgcccagt cgagcccgcg cgggcccggc gcgtagcggt
     4141 ccagccggtg cttgctgcgc tcgtggtagt cggaaagaga gtactcgggc atcgggtggg
     4201 cggaagggcg ccgcccccgt ataatttacc ctcatgagct actacgacaa gcacgtcttc
     4261 ttctgctgca accagcgcac caacggcgag gcctgctgca acgacaaggg cgcgagcgcg
     4321 atgcgcgact acgccaagaa gcgggtgaag gagctgggcc tcgccggcaa gggggccgtg
     4381 cgcatcaacc aggcggggtg cctcgaccgc tgcgaggaag gaccctgcat cgtggtgtac
     4441 ccggacgagg tctggtacac ctacgtcgac cgcgccgaca tcgacgagat catcgaggag
     4501 cacctcaaga acggccgggt cgtcgagcgc ctgcgcctat gagcgcgccg gccgagcgcg
     4561 tcttcgtcgc cggagacgcg ggccggatcg agaccgtgat cgaccttccc gccgcgtccg
     4621 gatccggcgc gccgcgcggc ctcgcgctgg tcgcgcaccc gcatcccctg tacggcggca
     4681 cgctcgacaa caaagttgca cagacgctcg cgcgcgcctt cgtcgagctc ggctacgtcg
     4741 cgctgcggcc caatttccgg ggggtcgggc aatccgaggg cgagcacgac gagggcgacg
     4801 gcgagaccga cgacctggtg aaggtcgcgc aatacgcctc ggggcgcttc ggcgccctgc
     4861 ccgtcgcgct cgccggcttc tccttcggcg ccttcgtgca gacgcgcgtg gcgaagcgca
     4921 tcgtccccga gcgcatggcc ctcgtcggac tcgccgtgag gcgtgccggc accgatacgg
     4981 tgccggccga taccgtcctg atccacggcg agcgcgacga caccgtcccg ctcgccgacg
     5041 tgctgcgctg ggccgagcct caggagctgc cggtggtcgt cattgccggg gcggaccatt
     5101 tcttccatcg ccggctgcac gtcatccggc gcatcgtcca ggacgcctgg cgctagggct
     5161 tgccctggcc gcgggcgcgg tcttatgctc gcgccggacg cccccttaag cgctgaactc
     5221 gtccccatgc tctgggtcaa gactctacac atcatcttcg tcgtcacctg gttcgcgggg
     5281 ctgttctacc tgccgcgcct ctacgtgtat cacgccatga ccgacgaccg gccgggccgc
     5341 gagcgcttca agatcatgga acgcaagctc tactggggca tcaccacgcc gggcggggtg
     5401 ctcgcgatcg tcttcggcgt ctggctctgg ctcggctggg gcttcggcgg cggctggctg
     5461 tacgcgaagc tcgcggtcgt cgcgctgctc gtcgcctatc acctctggtg cggaaagctc
     5521 ctggtcgact tccgcgaaga ccgcaacacg cgcagccacg tctggtaccg ctggttcaac
     5581 gaagcgccca cgctgctgct cctcgcgggc gtcgccctcg cggtggtgaa gccgttctag
     5641 gcgccgtgcc tgcgagcgtc accccagaat cgatccggca ggcggcggcg gtcatcgccg
     5701 gcagcgtcga gaagacgccg tgcttcgagt cgagggcgct ctccgaggcg accggcgcgc
     5761 gcgtgtggct gaagttcgag agccaccagt tcacgtcctc gttcaaggag cgcggcgcgc
     5821 tgaacaagct ctcgtcgctc tccgccgccg agcgctcggc cggggtgctc gcgatctcgg
     5881 ccggcaatca tgcgcagggc gtcgcctacc atgcgcagcg cctcggcatc cgcgcagtca
     5941 tcgtcatgcc gcgcctgacg ccgcacgtga aggtgcagcg cacgcggcgc ttcggcgccg
     6001 aggtgatcct catcggcgac acgttcgacg aggcgaagac cgccggcctc gccctcgccg
     6061 ccgagcgcag cctggtgctc atccacccct acgacgacga gcacgtcatc tcgggccagg
     6121 ggacggtcgc gctcgagatg ctggcggcgc agccggcgct cgataccctc atcgtgccgg
     6181 tgggcggcgg cgggctgatc gccggcatcg cggtcgccgc caagggaatc aatcccggca
     6241 tcgaggtgct cggcgtcgag accgagtcct acccggccgt gcattgcgcg ctgcgcggcg
     6301 tgccgcccgc gttcgcgcac acgatcgccg aaggtatcgc gctcaaggag cccggcaccc
     6361 gtacgctgcc catcatccgc gagaaggtct ccgacgtggt cctcgtttcc gagagcgaga
     6421 tcgagcaggc gatcgtgatg ctgctcgagg cggagaaggc cgtggtcgag ggcgcgggcg
     6481 cggcgccgct cgcggcgctg gtgcgctacg gcgagcgttt ccgcgggcgg cgaaccggcc
     6541 tggtcgtgtc ggggggcaac atcgagccac tgctgctctc ggagatcatc acgcgcggcc
     6601 tggtgaaggc cggccgcctg gcgcgcctgc gggtgcgggt acgggacttg cccgggtctc
     6661 tcgcccgggt caccgcctgc ctggcggcgg ccgacgcaaa catcgaggaa gtgcatcacc
     6721 agcgggcttt cgcggccgac ctgccgctgc agcaggtgga gatcgagttc gtgctgcaga
     6781 cgcgcggccc ggagcacgtg caggaactga tcgcggcgct ggaccgctcg ggtttcccgg
     6841 ctcgactgac gctcgattga cgggcctgtc gcccggctga cgagcgagcc gcattctcgc
     6901 tgctggaacc cgcaatatta cccgttcgga tgcccgcgtg gagtgctgct aatcgtgacc
     6961 gaaaacggta tgcttcatgc taagcttttc gcgtagggaa cgagaagcca gatcaagaaa
     7021 cagggagcgc ggcggaagcg atcatgacag cgaagaacat cactttgcgg cccggccggc
     7081 ccgacgacgc cgagaaaatc gcgatgatgt ctcgggacct gatcgaaacc ggcctcggat
     7141 ggtcctgggg cccggaccgc gtgagccgct cgctgcgcaa caaggacacg ctcaccgtga
     7201 tcgcctgcga ccgcgagcgc atcgtcgcgt tcgcgatcat gtacttcggc gaggagcacg
     7261 cgcacctcaa cctcctcgcg gttcgtccgt cgcaccagcg cctcggcatc ggacgccgcc
     7321 tcgtcgaatg gatggtggag tcggcgttct ccgcgggcat cgcgacgatc cacctcgagc
     7381 tgcgcgcgag caactacacg gcgcgcagct tctaccgcgc gctcggcttc gcggagtccg
     7441 cgtacattcc cggctactac cgcggccgcg agatggcgct gcgcatgatg cgggtgctgc
     7501 gcaagcccgg catgccgctg cccgcgtggc cgatcccgaa gctgcccaag agctgattcc
     7561 atcccggcgt cgcgcggcgc cggcgctcct gctggtcctc tcgctggtcg cgcccggggt
     7621 ctttgcgccc gaagccgtcg ccgccccgcc ggtcggcccc ggcatctcgc gcgcgctcgc
     7681 cgcgtggcgc gcgccgcgct accgcgacgt gcgctactcg ctcgatctcg aggtcgaccg
     7741 cgacgcggcc cggctcgccg ggaccgtgca gatcgagctc acgctcgcct cgccgccgcc
     7801 cgatgtcgtg ctcgactggc gcccggccgc aggcacccgg ctggaggatc tgcgcgtcga
     7861 cggcgcgccg gccgcggccg cgcgcgtcgt gcgcgaccac atcgtcgtcc ccgcgcgcct
     7921 gctgcacccc gggcgcaaca ccgtgcgcgc gcggatcgaa gcgccgatcg ccgtcgcggg
     7981 cggcgcgctc acgcgctacg aggaccacga ggacggctcg cgctacgtgt atacgctgtt
     8041 cgtgcccgcg gacgcgagca cggtgtttcc gtgcttcgac cagcccgatc tgaaaggccg
     8101 cttccggctc gagctggcgg cgccggcgtc ctggacggtg atcggcaacg gcccggcgcg
     8161 cgacgtcgag gaccggggcg cgacgaagcg ctggcgcttc gaggagacgc cgccgatcag
     8221 cacctacctg ttcgcattcg ccgcgggccc tttcgtcgag gcgggcaacg gcaccgacct
     8281 gcgcacgcgg ttgttcgtgc ggcgatcgcg cgccgagcgc gcgtcgcgcg aggcgggcga
     8341 cgtgctcgag cagaacgcgc gcgcgctcga ctggctggcg gagtacttcg accggccctt
     8401 cccgttcgcg aaatacgatc tcgtgctcgt gccggaattc gcctacggcg gcatggagca
     8461 cgcgggcgcc gggttcctgc gcgaggagtc ggtgctcttc ccgtccgagc cgaacgagaa
     8521 cgaccggctg cggcgcgcgc aactgctgtt ccacgaggcg acgcaccagt ggttcggcga
     8581 cctggtgacg atgaagtggt tcgacgacct gtggctgaag gaaggcttcg cgaacttcat
     8641 ggcggcgaag gcgaccgagg cgctgctgcc gaaggatccg gcgtggacgg cgttccacgc
     8701 gctcaaggtc accgcgtacc gaaccgacgt gacgcgcggg acgacgccga tcttccagcc
     8761 gctcgccaac ctggccgcgg cgaagtcggc gtacgggacg atcgtctacg gcaaggcgcc
     8821 ggccgtgctg cgccaggccg agttctacgt cggcgaggcc gcgttccgcg ccgcggtgcg
     8881 cgcgttcgtc gccgcgcacg cctacggctc tgcggactgg agcgacctgg tgcacatgct
     8941 cgagcgcgcc tcgcgcaagg acctgcgcgc ctgggcggcc gcgtgggtcc agcgccgcgg
     9001 catgccgcgc gtgcgcctcg cctggagcac ggacgcgagt ggcgcggtgc ggggggcgac
     9061 cctccgccag agcgacgtcc tgcgcgaggg cggcgtctgg ccgatgcgcg tgcgcctcgc
     9121 cgcggtcgcg cccgacggga gcgcgcgagg cttcgatgcg acgctgcgcg gccgccgcgc
     9181 gccgcttcct gcgctcgacg gcatccccgc gcccgcgctg ctcttcgcca acgacggcga
     9241 ctacggctac gggcagttcc tcctcgacgc gaaaagccgc gcgaccgtgc tcgcccgccc
     9301 ggacctcgcg cgcgatgcgc tgctgcgctc gctcgtgttc gacgcggtct gggactcggt
     9361 gcgcgaggcc gagctcgccc cgcgcgagta catcgacttc gccctgcgcg ccgcgccggc
     9421 cgagcgcgat ccggtcacct tgtcgacgct cctcgcgcgc gtcgaggcgg cgttccgggt
     9481 ctatctttcc gacgcgcagc gcgacgcggt ggcgcccgcg ctcgagcgga cgctcgtcaa
     9541 cggcatgctc ggcgccgact ccgccggaag gcggatcagc ttctttcgcg cgttcgccga
     9601 tagcgcctgg tcgcccgagg gccgagccgt gatcaagcgg ctgctcgcgc gcgcgctcga
     9661 ggttccgggc gttccgcttt cgtcgcggga ccggttccgc ctggtcgcgc ggctgctcgt
     9721 cctcggcgac ccggacggcg agacgctgct cgcggcgcag gcggaggcgg acccgggcga
     9781 cgacgggcgg cgctacgcgt acgcggccgg cgccgcgcgt cgcgacgccg cggtcaagtc
     9841 ccggtacttc gagcgcgctc tgcgcgacgc cgcgctgccc gagagctggg tggaggcggc
     9901 gctgggggcc cagaacgcgc ccgagcacgc cgcgctcacc gcggcgctgg tcgagcccgc
     9961 gctgcgcgcg ctgcccgagc tcaagcgctc gcgcaagatc ttcttcgtga acgagtggct
    10021 gtcggcgttc ctgggcggcc agcgggacgc ggaagtgctc gccgtggtcc ggcgcttcct
    10081 gcaaaggccg gagctcgatc gcgacctgcg gctcaaggtg ctcgaggcgg tcgatccgct
    10141 cgagcgcgcc gtgcgcatcc gcgcgaagtt cgccgccgga tgaccgaccg cttcgatctg
    10201 ttcgcgacct gcccgcgcgg gctggaagcc ctgctcgcgg aggagctcgg ggcgctcggc
    10261 gcgcgcgagc cgcgcacggt gccgggcggg gttgcgttcc gcggcgacct cgccgcgtgc
    10321 tactcggcca acctcggctc gcgcttcgcg acgcgcgtgt tgctgcggct cggcaccgcg
    10381 ccgtaccggc gcgagcagga cgtctacgac gccgcgctcg cgctgccgtg gccggagtgg
    10441 ttcggcgagc ggcagacgat ccgcgtcgac ctgaacgcga cgcgctcgcc gctgaagagc
    10501 ctcgacttcg ccacgctgcg cgtcaaggac gccgtgtgcg atcgtttccg cgccgaccgc
    10561 ggcagccggc ccgatgtcga tacgcgctcg cccgacgtgc gcatccactg ctatctcgac
    10621 gcgaagcagg cgatgttcta tctcgatacg tcgggcgagc cgctctacaa gcgcggctac
    10681 cgcaaggaca cgggcgaggc tcctctgcgc gagaacctcg cggccgggat cgtggcgctc
    10741 accggctggg acggggtcga gccgctcctc gacccgatgt gcggcaccgg caccctcgtg
    10801 atcgaggcgg cgctgaaggc gctcgcgatc ccccccgggc acgcgcgcgg cttcggcttc
    10861 gagcggctgc gcgtccacga cgcgaagcag tgggagcggg tgcgtgacgg gatgcttgcg
    10921 aagcggcaaa gccggccgct cgccgtattc ggccgcgacc gctacggcga cgagctcaag
    10981 aaggccgagc gcaacgtcga ggcagccgga ctctccgggt cgatcgagct caagcaggcc
    11041 gacgtcctcg agagctccgc gccggcgaaa agcggactcc tcgtcgccaa cccgccttac
    11101 ggcgtgcggc tcggcgacaa gaacgagctc gccgcgttct atcccaggct cggcgacgcg
    11161 ctcaagcggc actacgccgg ctggcggtgc ttcgtcttct cgggcgatcc gcagctcgcg
    11221 aagctgatcc gcctgcatcc ctcgcgcaag atcccgctct acaacggcgc gctcgagtgc
    11281 cggctgtacg agtaccggat ggtcgccggg tccatgcgca aggatgcgcg gccgcgctag
    11341 cccgccgcgg cgcgccagag cagcgacgcg ccggtcgcga tcaacagcgc cgcgatgaag
    11401 cggccgagcg cgcggcgcga gatgcgcagg tgcgcgcgcc cgccggccca caggcccgcg
    11461 aagaggaagg gcaggagcac cagcgccgtc ttcagctcgt tccacgcgac cagtcccgcg
    11521 atcgcgaaca tgaccaagcg gctgccgacg ctgaagatca ccatggtggc gaccgtggcg
    11581 cgcagcacgt ccttgtcctc gatgcgccgc gagagataga tcgcgtaagg cggaccgccg
    11641 atgccgaaca gcgcgccgct cgtgccgccg acgatgccgg cgacgtaggc ccagttgcgg
    11701 ctgatccggc cgaacgcccc gcgctcgacg agcgcgtaga agccgtagag caccgcgaac
    11761 gcgccgatgc cggcgagccc agcgccgcgc ggaatgttga cgagcagcgt cgcgcccagc
    11821 gccgcgccga ggaccgccat cgggatcatg cgcgtcagct cgccgcgctc ggcctcgcgg
    11881 tggtggcgca cgccgaggaa catcgacgcg gtgaagtcga gcagcgccag caccggcagc
    11941 acgaactgca ccggccacag atgcgagacg accggcacgg tgaccagcgc cgccccgaaa
    12001 cccgacagcc cgaacacgaa gtagccgcac gcgacgaccg cggccgcgac ggcgagggac
    12061 tcccagctga ccggcaaggc gctagcgcat cgggctgtgc gtgctcgtca ccgcgacgaa
    12121 gatctcgcgc aggaacacgc acaggctgcc gatgaaggac agcatcgccg cgatgaacag
    12181 cagcccgacc gtatgccaaa ggtcgaccgc cacgagcgca tcgacgaacg cgacgatgat
    12241 cagcaggccg acgaacaggc cacagagcac tgccgccgtc atcgccgtat acacgagccg
    12301 catgcgacgg gaaaggaccg cgagctcgct gtcgcacgca gcgcgcgcct tgacatccag
    12361 gctcggggcg agctcgtcga gcatccggat ccggtcgacc acgcgcccga gccggttcga
    12421 cagcaccccg agcagcgttc ccaccgcggt cagcaggaac accggcgcga ccgcgagcga
    12481 gatgagatgc gagatatcgg ggtgctggac caccgatcag gccatcgcct tgcgcagcgc
    12541 gtgcttgaaa tggtggatgt ggtcgagcgc gaccttcagc tgcttgcggt ccttcgcctt
    12601 cagcgcctcg tcgcgcttct tctgccactc gcggatcttg cccttcaccg cggccttgtc
    12661 gatgcccttc acctcgtggt gaacgtgcat gtcgatgttg agcgccttgc agatcgcggc
    12721 gagcaggtgg tccttgttca tctgcgtcgc gccctgcacg gcgtcgtgct ggatgccggc
    12781 ggcgatctcg cgcagctcgg cgaggttttt gctcttcagt tccgggtagg tgtgtgaggg
    12841 ttctgccatg tgggcctcct tcgggtccga accgaaatct tagcgcgaaa cgaaaaaggg
    12901 gcgctgccgc gcgccccttt ctgcgacacg tccgccgctc agacgatatt gaggttctgg
    12961 atcccttccg tgagatcgcg gttcttcgct tccttcgact gcagcttgat ggcgagccgc
    13021 aggtcgttga cgctgtcggc gttgcgcagc gcgtcctcgt agctgatctt gcccgcctcg
    13081 tac
//
LOCUS       DATDMW010000140        10520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2159257, whole genome shotgun sequence.
ACCESSION   DATDMW010000140 DATDMW010000000
VERSION     DATDMW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159257"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VI319_06135"
     CDS             <1..378
                     /locus_tag="VI319_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEY6863461.1"
                     /translation="RFPGNNGHTEVPNMSMNLNQGASASGAGGSTAAPETWNLRLYIA
                     GQTPRSLAAFANLKTLCETHLKGRYTIEVVDLMQHPQLAEGDQIVAIPTLVRKLPPPM
                     KKIIGDLSNEEKTLVGLDLIPRR"
     gene            380..664
                     /locus_tag="VI319_06140"
     CDS             380..664
                     /locus_tag="VI319_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEY6863462.1"
                     /translation="MSAEPLRFSFVLYVAGAGARSLRAIQNAKAACEELLAGRYDLEI
                     VDIYKHPERARDDQIVAVPTLERKRPVPLRYLIGDLSVRERLVESMGMAA"
     gene            661..2292
                     /locus_tag="VI319_06145"
     CDS             661..2292
                     /locus_tag="VI319_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6863463.1"
                     /translation="MSATPDNVIPFDSEPLEALRARLAEAEEKLEALRTGAVDALVVG
                     GPDGPKVYVLQSADHPYRMLVEQMQDAALTLDKTGEVLYCNSCFGELAGVPGERILGR
                     RLGDFVQSEDRLLLETALATARNNPTRVELHLLGPEGHGTPAHFAFSPLRTEQFDGIC
                     LIVTDLTEHYRARELIASGRKKDEFLAMLAHEFRNPLAPIRHAVDVLRLLEPGDERFG
                     QMRDLIGRQTHHLSRLVDDLLDISRVTQGKINLQIQPVDVNRVLSEGVELARPLIDER
                     RHRLTLRPLIEPARVAGDFTRLVQVVGNLLNNAAKYTAPGGEILASVERDGRWVAIRV
                     VDNGMGIAADLLPRVFDLFTQASRAPDRAPGGLGIGLSLVHSIVALHGGSVEAKSDGA
                     QKGSEFVVRLPLSAERCDAASDAEPRPVGRSSRRIVVIDDNRDAADSMATLLQLRGHA
                     VQVAYDGPSGLALALAHRPHVAFVDIGLPGLDGYELARQLRARGAGLVLVALTGYGRD
                     EDRILSKDAGFDHHLVKPVPGEALERLLADAPGSG"
     gene            complement(2296..3147)
                     /locus_tag="VI319_06150"
     CDS             complement(2296..3147)
                     /locus_tag="VI319_06150"
                     /inference="COORDINATES: protein motif:HMM:NF027432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4824 family protein"
                     /protein_id="HEY6863464.1"
                     /translation="MKRSHTRLAGLVLLALTNAIALGGALWNRSGEPQARMRLTEREL
                     QRFEPWYGNRENSGLTLRLRWRTLVEERTAADFYSLRYGSSGGAPGWLDRAKMESLGF
                     DTSMLAAYSERARKRYEKQLPREVLLVLELDGAAYRRALELTRERVDGELAKPFIAGD
                     RAAEERRKSLRETLDWETSRSSRLFVVDAGTDADALRAKYQDRARYAIVRGEARPWAA
                     GAGSGRAAGIVDSVSVASINVPLRLRGVLAGVQALDYAPAPRTPFDATVAWGRRFEPW
                     LVEAARR"
     gene            complement(3144..4211)
                     /locus_tag="VI319_06155"
     CDS             complement(3144..4211)
                     /locus_tag="VI319_06155"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEY6863465.1"
                     /translation="MSASSRSEAQQRADEIRAFRAELERLEREGVLVLAPEQRRAVEA
                     HHGELLAGYARAFDIDRDAEAKQLSLGMRVASFLGALALAASVFFLFYQFWGLLPTAA
                     QVGILLAAAFGTFLATLLVQARDPTGYFTKLAAMVAFACFVLNIAMLGQIFDITPSDK
                     ALLPWAAFALLLAYACDLRLLLAAGILCLIAFISARTGTWSGMYWLYFGERPENFFPA
                     AVAMFLVPQLVDHRRYPGFGAIYRVFALLSLFLPVLVLSNWGEISYIDADDKIIEGAY
                     QVLGFAVSGGAVWLGAKRHWPEAVNTGLVFFVVFLYTKFYDWWWEIMPKWLFFLVIAL
                     TAVLLLLVFKRVRTVRVGAAG"
     gene            4342..5211
                     /locus_tag="VI319_06160"
     CDS             4342..5211
                     /locus_tag="VI319_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY6863466.1"
                     /translation="MTPYPDPHAFHSELCRFNAGRLTPARIRPDWEADIRAELDMRAT
                     EGYFVEVERARAFERAAQAPWDAAGFVDWFEDLRRCGPGQGDPLFPWLAERATLEEMR
                     WFLTQEAAGEAGFDDLVAMAQVKLQPVRAKLEMARNYWDEMGRGHERGMHGPMLHTVV
                     RELDLRPRIEDTVWESLALANLMVALAANRRYAYQAIGALGVIEMTAPGRVGLVNDGL
                     RRLGVATDARLYFQLHSELDIKHSKAWNAEVLYSIVDSDPELAQPIAEGALMRLACGA
                     RCFERYRRHFGLT"
     gene            complement(6606..7205)
                     /locus_tag="VI319_06165"
     CDS             complement(6606..7205)
                     /locus_tag="VI319_06165"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6863467.1"
                     /translation="MPAPVFVCAHGAGGHKDDRGMLALAKVLEARGVEVVRFDFPYRE
                     RKSGRPDPMPVLEATVAEVVARVREQRRPDGLVIGGRSMGGRAASMLAAKGFQCDGLL
                     LLAYPLHPAGQPEKLRDAHLASIEAPVLCLNGTRDALCRRDLMEKAVAGLSSWTMHWL
                     DGADHSFRVLKSSGRTDADVLDEIGDESGAWLESLAHRR"
     gene            complement(7205..8182)
                     /locus_tag="VI319_06170"
     CDS             complement(7205..8182)
                     /locus_tag="VI319_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY6863468.1"
                     /translation="MKITILDDYFDTLRTLDCFAKLAGHDVTVWNDHVQDTDRLAERL
                     KDAEAIVLIRERTKIQAPLLERLPKLRLISQRSVYPHIDVAACTRLGVIVSSFQHDGT
                     PSYATAELAWGLILAAMRRIPQQVEALKRGAWQLGVFGSVRGKTLGVHSYGRIGAVVA
                     GYGRAFGMRVVAWGREASRQRAERDGYVAARSREEFYEQCDVISLHMRLVDATRGIVT
                     RADLARMKPTALLVNTSRAGLIEPGALVDALEAGRPGLAAVDVYEQEPIRDGSHPLFA
                     MDNVVCTPHVGYVTREEYETQFSDVFDQIVAYAAGAPINVVNPEVLKRR"
     gene            8362..9519
                     /locus_tag="VI319_06175"
     CDS             8362..9519
                     /locus_tag="VI319_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863469.1"
                     /translation="MKRRRLVVVGTLAANPYAGMAWMHMQIVLGLMRLGHEAWYFEAS
                     SGWPYDPVRGTAVCDSDYAVPYLARTAEAFGLGDRWAYRRSYSDKEWLGMPRDKAEAL
                     LAGADAVFNVAGATKFAEEGLKVGRLVYFGTDPVADEIAVSRGEKQWLVEEHHDFVTY
                     GERIGRPGCAIPPLPRLRARTRQPMILDLWAGTQPTRDAYTTVGNWRQDGEVEFRGDT
                     YYWSKDREFRKFIALPRRVARPVEIALGVHDLDPDSRALLDANGWRLADAHGFTQDPW
                     KYRDYVRASRAEFTVAKDQNVRLRSGWFSERSASYLAAGRPVITQNTGFGDVLPTGEG
                     LFAFDTLAEIVAAFEAIESDYARHARAARLIAEEYFRAETVLERLLRDLGL"
     gene            9519..10274
                     /locus_tag="VI319_06180"
     CDS             9519..10274
                     /locus_tag="VI319_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEY6863470.1"
                     /translation="MAARSLIVNADDFGQTPGVNRGIIAAHENGIVTSASLMTHGAAV
                     REAVDYALSHPALGLGLHADLGEWFFRDGAWHARYQRVRVEDAGEVARELAAQLDAFQ
                     RLTGRAPTHLDSHQNVHMREPARSCFAQAAARWNIPLRACAPGFHWCGDFYGQTAEGL
                     PFPQAIRPESLVRIIETLPAGVTVLVCHPAERADLETTYLHERRIELATLCDRRVRAA
                     LASRGVALRSFEESAAETKSPDRAVRARAARAL"
     gene            complement(10271..>10520)
                     /locus_tag="VI319_06185"
     CDS             complement(10271..>10520)
                     /locus_tag="VI319_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863471.1"
                     /translation="GLFVRVSASFGILLMLVYWTAHMSFPYIENKNNFLLDFHIVYAV
                     VLVQLIVERAGHVWGLDGWAEKIDFFGRHPKLKAALVA"
BASE COUNT         1596 a         3790 c         3666 g         1468 t
ORIGIN      
        1 agattccccg ggaacaacgg ccacacggag gtgcccaaca tgtctatgaa cctgaaccaa
       61 ggcgcgagcg cctccggcgc cggcgggagt acggcggcgc cggaaacctg gaaccttcgg
      121 ctctacatcg cgggccagac gccccgctcg ctcgccgcgt tcgcgaacct gaagacgctg
      181 tgcgagaccc acctcaaagg ccgctacacc atcgaggtgg tcgacctcat gcagcacccg
      241 cagctcgccg agggggatca gatcgtcgcc atcccgaccc tggtgcgcaa gctccctccg
      301 ccgatgaaga agatcatcgg cgatctgtcc aacgaggaaa agacgctggt ggggctggat
      361 ctcatcccgc gtcgatagcg tgagcgccga gccgctccgc ttctcgttcg tgctctacgt
      421 cgcgggcgcg ggcgcgcgtt cgctgcgcgc gatccagaac gcgaaggcgg cgtgcgagga
      481 gctgctcgcc gggcgctacg atctggagat cgtcgatatc tacaagcatc cggagcgcgc
      541 ccgcgacgat cagatcgtcg ccgtgccgac gctggagcgc aagcgccccg tcccgctgcg
      601 ctacctcatc ggcgacctct cggtgcgcga gcggctcgtc gagagcatgg ggatggccgc
      661 atgagcgcga cgccggacaa cgtgattccg ttcgacagcg agccgctcga ggcgctgcgc
      721 gcgcggctgg ccgaggccga ggagaagctc gaggcgctgc gcaccggcgc ggtcgacgcc
      781 ctggtggtgg gcgggcccga cggccccaag gtctacgtgc tgcagagcgc cgatcacccg
      841 taccgcatgc tggtcgagca gatgcaggac gcggcgctca cgctggacaa gaccggcgag
      901 gtcctctact gcaactcgtg cttcggcgag ctggcgggcg tgccgggcga gcgcatcctg
      961 ggcaggcgcc tcggggactt cgtgcagtcc gaggaccggc tgctgctcga gacggcgctc
     1021 gcgaccgcgc gcaacaaccc gacgcgcgtc gagctgcacc tgctcggccc cgaagggcac
     1081 ggcacgcccg cgcatttcgc tttcagcccg ctgcgcaccg agcagttcga cggtatctgt
     1141 ctcatcgtca ccgacctcac cgagcactac cgcgcgcgcg agctgatcgc ctctggacgc
     1201 aagaaggacg agttcctcgc catgctcgcg cacgagttca ggaacccgct cgcgccgatc
     1261 cgccacgcgg tcgacgtgct gcgcctgctc gagcccgggg acgaacgctt cgggcagatg
     1321 cgcgatctca tcgggcgtca gacgcaccac ctgtcgcgcc tggtcgacga cctgctcgac
     1381 atctcgcgcg tcacccaggg caagatcaac ctgcagatcc agccggtgga cgtcaatcgc
     1441 gtgctctccg agggcgtcga gctcgcgcgc ccgctgatcg acgagcgccg gcaccgcctg
     1501 acgctgcgtc cgctgatcga gcctgcgcgc gtcgcgggcg acttcacgcg cctggtgcag
     1561 gtcgtcggca acctcctcaa caacgccgcg aaatacaccg cgccgggcgg cgagatcctc
     1621 gcgagcgtcg agcgcgacgg ccgctgggtc gcgatccgcg tcgtcgacaa cggcatgggc
     1681 atcgcggccg acctgctccc gcgcgtgttc gacctcttca cgcaggcgtc gcgcgcgccc
     1741 gatcgcgcgc cgggcgggct gggcatcggc ctgtcgttgg tgcacagcat cgtcgcgctg
     1801 cacggcggct cggtcgaggc gaagagcgac ggcgcgcaga aaggcagcga gttcgtcgtg
     1861 cggctgccgc tgtcggccga gcgctgcgat gcggcctccg acgccgagcc gcggcccgtg
     1921 ggcaggagct cgcgccgcat cgtcgtcatc gacgacaacc gagacgccgc cgacagcatg
     1981 gcaacgctgc tccagctccg gggacacgcg gtgcaggtcg cgtacgacgg cccgagcggg
     2041 ctcgcgctcg cgctggcgca ccggccgcac gtcgccttcg tggacatcgg tttgcccggc
     2101 ctcgacggct acgagctcgc gcgccagctg cgcgcccgcg gtgcggggct cgtgctcgtg
     2161 gcgctgaccg gatacgggcg cgacgaggac cggattctct ccaaggacgc gggcttcgat
     2221 caccacctgg tcaagcccgt gcccggcgag gcgctcgagc ggctgctcgc ggacgcgccc
     2281 ggctcgggct gagcgtcacc ggcgcgcggc ctcgaccagc cagggctcga agcgcctgcc
     2341 ccatgcgacc gtcgcgtcga acggcgtcct cggcgcgggc gcgtaatcga gcgcctgaac
     2401 gcccgcgaga actccgcgca gccgcagcgg aacgttgatc gaggccacgc tcacgctgtc
     2461 gacgatgcct gcggcgcggc cggagcccgc gcccgcggcc cacggcctcg cctcgccgcg
     2521 aacgatcgcg tagcgggcgc ggtcctgata cttcgcgcgc agcgcgtcgg cgtcggtccc
     2581 ggcgtcgacg acgaagaggc ggctgctgcg cgaggtctcc cagtcgagcg tctcgcgcag
     2641 gctcttgcgc cgctcctcgg cggcgcggtc gccggcgatg aagggcttcg ccagctcgcc
     2701 gtcgacgcgc tcgcgcgtga gctcgagcgc gcggcggtac gccgcgccgt cgagctcgag
     2761 caccagcagc acctcgcgtg gcagctgctt ttcgtagcgc ttgcgcgcgc gctcggaata
     2821 cgccgcgagc atcgaggtgt cgaatccgag cgattccatc ttggcgcggt ccagccatcc
     2881 gggagctccg cccgaggagc cgtaacgcag cgaatagaaa tccgccgctg tccgctcctc
     2941 gaccagcgtg cgccagcgca agcgcaacgt cagaccgctg ttctcgcggt tgccgtacca
     3001 cggctcgaac cgctgcagct cgcgctcggt caggcgcatg cgcgcctgcg gctcgcccga
     3061 gcggttccac aacgcgccgc cgagcgcgat cgcgttcgtg agcgcgagca ggaccaggcc
     3121 ggcgaggcgc gtgtgcgacc gtttcatccg gcggctccta cgcgcaccgt gcgcacgcgc
     3181 ttgaacacga gcagcagcag caccgcagtc agcgcgatga ccaggaagaa cagccatttc
     3241 ggcatgatct cccaccacca gtcgtagaac ttggtgtaga ggaacacgac gaagaagacg
     3301 agcccggtgt tgacggcctc gggccagtgg cgcttcgcgc cgagccacac cgctccgccg
     3361 gagaccgcga agccgagcac ctggtacgcg ccctcgatga tcttgtcgtc cgcgtcgatg
     3421 tagctgatct cgccccagtt cgacagcacg agcacgggaa gaaacagcga caggagcgcg
     3481 aacacgcgat agatcgcccc gaagcccggg tagcgccggt gatcgacgag ctgcggcacc
     3541 aggaacatcg cgaccgctgc cgggaagaaa ttctccgggc gctcgccgaa gtagagccag
     3601 tacatcccgc tccacgtccc ggtccgcgcc gagatgaacg cgatcaggca caggatgccg
     3661 gcggcgagca gcagcctgag gtcgcacgcg tacgcgagca gcagcgcgaa cgccgcccag
     3721 ggcagcagcg ccttgtccga cggcgtgatg tcgaagatct gcccgagcat ggcgatgttc
     3781 agcacgaagc acgcgaacgc caccatcgcg gcgagcttgg tgaagtagcc ggtcggatcg
     3841 cgcgcctgga ccagcagcgt cgcgaggaag gtgccgaacg cggccgcgag caggatcccg
     3901 acctgcgccg cggtcggcag cagcccccag aactggtaga agaggaagaa aacgctcgcc
     3961 gccagcgcga gggccccgag gaaggaggcg acgcgcatgc cgagcgacag ctgctttgcc
     4021 tcggcgtcgc ggtcgatgtc gaaggcgcgg gcatagcccg cgagcagctc gccgtggtgc
     4081 gcctcgacgg cgcggcgctg ctcgggcgcg agaacgagaa cgccttcgcg ctccaggcgc
     4141 tcgagctcgg cccggaaggc gcgtatctcg tcggcgcgct gctgcgcttc gctgcgcgaa
     4201 gaggcgctca tcttctctcc cgccgcgact ataccattcg gcgcccgcca atcggtctac
     4261 accgggaaac ggcgcggcga aatccgtcgc ccggaagata cagtcctgtt cagcctatca
     4321 ggctccaaag gatcactgtc cgtgacgcct tatcccgatc cccatgcgtt ccattcggag
     4381 ctgtgccggt tcaacgccgg caggctgacc cccgcccgca tccgtccgga ctgggaggcc
     4441 gacatccgcg cggagctgga catgcgcgcg accgaaggct acttcgtcga ggtcgagcgc
     4501 gcccgcgcgt tcgagcgggc ggcgcaggcg ccctgggacg cagccggctt cgtcgactgg
     4561 ttcgaggacc tgcgccgctg cggcccgggc cagggcgacc cgctgttccc gtggctcgcg
     4621 gagcgggcga cgctcgagga gatgcgctgg ttcctgaccc aggaggccgc gggcgaggcc
     4681 ggcttcgacg acctggtggc gatggcccag gtgaagctgc agccggtccg cgcgaagctc
     4741 gagatggcgc gcaactactg ggacgaaatg ggccgcggcc acgagcgcgg catgcacggc
     4801 ccgatgctgc acaccgtggt gcgcgagctc gacttgcgcc cgaggatcga ggacaccgtc
     4861 tgggagtcgc tcgccctcgc caacctgatg gtcgcgctcg cggcgaaccg gcgctacgcc
     4921 taccaggcga tcggcgcgct cggggtgatc gagatgacgg cgccgggacg cgtcgggctc
     4981 gtgaacgacg ggctgaggcg cctcggcgtg gcgaccgacg cacggctcta cttccagctg
     5041 cactccgagc tcgacatcaa gcattcgaag gcctggaacg ccgaggtcct gtattcgatc
     5101 gtcgactcgg atcccgagct ggcgcagccg atcgccgagg gtgccctgat gcggctcgcc
     5161 tgcggcgcgc gctgcttcga gcgctaccgc cgccacttcg ggctgacgtg acgccgtcgg
     5221 cagcaacgct gctgcggcgc tggaccgcgg cgggcgtccc ctggaccgtc gccggcgacc
     5281 cgcggcttct cctgaagtac gccctggccg gcgcgctgcg cgcgcgctcg ccgctcgtcg
     5341 cgcggcgctg cgccgggctg ctgcgccgcg agcgcgccga tccggcgctg caccgggcgc
     5401 gcgccggggc gctgatcgcg cgcacgctgc gcgcggcgcg ccgcatcccg gcgcacgcgc
     5461 aaatcgcggc gcgcgccccg gcgtccgacc tgctcgcctg gctctcgagc gaggtcccga
     5521 tcatggacaa ggcgtcgctc aagacgcagc gcgcggcgta ctacccgaac cgcggccggc
     5581 ggctgccgtg gtggccgctc ggcgagagca gcggcagcac cggcgctccg accgacgtct
     5641 tccgcacttt cgactcgact ctctgggagg aagccttcca tcgccagcat tggcgctggg
     5701 cgggcgtgag cgccgccgag cgtcaggccg tgctgcgcgg cgaccgcgtg aagccgctcg
     5761 aagaccgcga gccgccgtac tggctcgagg accgcgtcgg ccggcagctc gtgctgtcca
     5821 cgcggcacct ggacgagcgc accgcgcgcg agttcgccga ggccatcgcg cgcttcggcg
     5881 ccgtgcagct gcgcgcctac ccgtcggcgg cgtacgcgct cgcgagcgcg ctcgaaggcc
     5941 ggcggccgcg tctgcgcctg tggtcggtga tcaccggctc ggagatgctc tacccgttcc
     6001 agcgcgcgaa gatcgaggcg gcgctcggag cgcgcgtgta ctcgttctac ggcatgtccg
     6061 agcgcgtcgt cttcgccgcg gagtgcgagc acgggcgcat gcacgtgaac ccggaatact
     6121 ccgtcgtcga gatcgtggac gggcaaggac ggcccaccga ggacgaaggt ttcctcgtcg
     6181 gcaccacgct gcacaacgag gcgatgccgc tcctgcgcta ccggctcacg gacaccgcgc
     6241 gctgggaccg gcggccctgc ccgtgcgggc gcacgtaccc ggtgatcgag gcgctcgctg
     6301 gcaagctcga ggaccgcgtc tacgacctgc gcggaaggcc gatcaccgcc gcggtcgtcg
     6361 gcttcgcgta caagggcctg ctcaacatcc gcaagggtca gatcgcgcag atcgcgcgcg
     6421 accgctgggt ggcgcggctc gtcccggggc ccgggtacgg cgccgccgac gccgagcgct
     6481 tcctcttgaa cgttcgcacg tacgtctcgg ccgacgtcaa cgtcgcgatc gagcgggtcg
     6541 acgacatccc cgcgctgccg aacggcaagt acaagtgggt gtcgcaggag tggcgcggcg
     6601 cagcctcagc gccgatgcgc gaggctctcg agccaggccc cgctctcgtc cccgatctcg
     6661 tccagcacgt cggcgtcggt cctcccggag gacttgagca cgcggaagga atgatcggcg
     6721 ccgtcgagcc agtgcatcgt ccacgatgac aagccggcca cggccttctc catgagatcg
     6781 cggcggcaca gcgcatcgcg cgtgccgttg agacagagaa cgggcgcctc gatcgaggcg
     6841 aggtgtgcat cgcgcagctt ctccggctgc cctgccggat gcagcggata cgcgagcagc
     6901 agcaagccgt cgcattggaa gcccttcgcc gcgagcatcg acgccgcgcg gccgcccatc
     6961 gagcgcccgc cgatgacgag cccgtccggc cggcgctgct cgcgcacgcg cgcgacgacc
     7021 tccgcgacgg tcgcctcgag caccggcatc ggatcgggtc ggccggattt gcgctcgcgg
     7081 tacgggaaat cgaagcgcac gacctcgacg ccgcgcgctt cgagaacctt cgcgagcgcg
     7141 agcatgccgc ggtcgtcctt gtggccgccc gctccgtgcg cgcagacgaa taccggcgcc
     7201 ggcatcagcg acgcttcagg acctcgggat tgacgacgtt gatcggcgcg cccgcggcat
     7261 acgcgacgat ctggtcgaac acgtcggaga actgcgtctc gtactcctcg cgcgtgacgt
     7321 agcccacgtg cggcgtgcac acgacgttgt ccatcgcgaa cagcgggtgc gagccgtcgc
     7381 ggatcggctc ctgctcgtag acatcgacgg cggcgaggcc cgggcgcccg gcctcgagcg
     7441 cgtcgaccag cgcgcccggc tcgatcaggc cggcgcggct cgtgttgacg aggagcgccg
     7501 tcggcttcat gcgcgcgagg tccgcgcgcg tcacgatgcc gcgcgtcgcg tcgaccaggc
     7561 gcatgtgcag cgagatgacg tcgcactgct cgtagaactc ctcgcggctg cgcgcggcga
     7621 cgtagccgtc gcgctctgcg cgctggcgcg acgcctcgcg cccccatgcg accacgcgca
     7681 tgccgaaggc gcgcccgtag cccgcgacca ccgcgccgat gcggccgtag ctgtgcacgc
     7741 cgagcgtctt gccgcgcacc gagccgaaca cgccgagctg ccatgcgccg cgcttgagcg
     7801 cctcgacctg ctgcgggatg cgccgcatcg cggcgaggat cagcccccag gcgagctcgg
     7861 ccgtcgcgta cgaaggcgtg ccgtcgtgct ggaacgacga aacgatcacg ccgaggcgcg
     7921 tgcacgccgc gacgtcgatg tggggataga cgctgcgctg gctgatcagg cgcagcttcg
     7981 gaagccgctc gagcagcggc gcctggatct tcgtgcgctc gcggatcagc acgatcgcct
     8041 cggcgtcctt caggcgctcc gcgaggcggt cggtgtcctg gacgtggtcg ttccacaccg
     8101 tgacgtcgtg gcccgcgagc ttcgcgaagc agtccagcgt gcgcagggtg tcgaaatagt
     8161 cgtcgaggat ggtgattttc atgatgcggg gattatcgcg ccttcggcgc cttcgggttt
     8221 accctgggcg cggagcacgc gctcttcgcc gtttcgcaag ctcaggcagg ccgttatgac
     8281 tagagtttgc ctcaagcgcg cgcacgcaat cgaaagctat agtcggctcg ccatggcccg
     8341 ctcgccgtcc cgccgtccgc gatgaaacgc cggcgtctcg tcgtcgtcgg aacgctcgcc
     8401 gcgaatccct acgccggcat ggcctggatg cacatgcaga tcgtgctcgg cctgatgcgg
     8461 ctcgggcacg aggcctggta cttcgaggcg agctccggct ggccctacga cccggtgcgc
     8521 ggcaccgccg tctgcgactc cgactacgcg gtgccctatc tcgcgcgcac cgccgaagcc
     8581 ttcggcctcg gcgatcgctg ggcgtatcgc cgcagctact cggacaagga atggctcggc
     8641 atgccgcgcg ataaggccga ggcgctgctc gccggcgccg acgcggtctt caacgtcgcc
     8701 ggcgccacca agttcgccga agaaggactg aaggtcggcc ggctggtgta cttcggcacc
     8761 gatccggtcg ccgacgagat cgcggtgagc cgcggagaaa agcagtggct ggtcgaggag
     8821 caccacgact tcgtcactta cggcgagcgc atcggccggc ccggctgcgc gatcccgccg
     8881 ctgccgcgcc tgcgcgcgcg cacgcggcag ccgatgatcc tcgacctgtg ggccggcacg
     8941 cagcccacgc gcgacgcgta tacgaccgtc ggcaactggc gacaggacgg cgaggtcgag
     9001 ttccgcggcg atacgtacta ctggagcaag gaccgcgagt tccgcaagtt catcgcgctg
     9061 ccgcgccgcg tcgcgcggcc ggtggagatc gccctcggcg tgcacgacct cgacccggac
     9121 agccgcgccc tcctcgacgc gaacggctgg cgcctcgccg acgcccacgg cttcacgcag
     9181 gacccctgga agtaccgcga ctacgtgcgc gcgtcgcgcg ccgagttcac cgtcgccaag
     9241 gaccagaacg tgcgcctgcg cagcggctgg ttcagcgagc gcagcgcgag ctacctagcc
     9301 gccgggcgtc ccgtgatcac gcagaacacc ggcttcggcg acgtgctgcc gaccggcgag
     9361 ggcctgttcg ccttcgacac gctcgcggag atcgtcgcag cgttcgaggc gatcgagtcc
     9421 gattacgccc gccacgcgcg cgcggcgcgc ctcatcgccg aggagtactt ccgcgccgag
     9481 accgtgctcg agcgcctgct gcgcgacctg ggattgtaat ggcggctcgc tcgctcatcg
     9541 tcaacgccga cgacttcggg cagacgccgg gcgtgaaccg cgggatcatc gccgcgcacg
     9601 agaacggcat cgtcaccagc gcgagcctca tgacgcacgg cgccgccgtg cgcgaggcgg
     9661 tcgactacgc gctctcccat ccggcgctcg ggctggggct gcacgccgac ctgggcgagt
     9721 ggttctttcg cgacggcgcc tggcacgcgc gctaccagcg cgtccgcgtc gaggacgccg
     9781 gcgaggtcgc gcgcgagctc gcggcgcagc tcgacgcctt ccagcgcctg accggccgcg
     9841 cgccgacgca cctcgactcg caccagaacg tgcacatgcg cgagccggcc cggagctgct
     9901 tcgcgcaggc ggccgcgcgc tggaacattc ccctgcgcgc ctgcgcgccc ggcttccact
     9961 ggtgcggcga tttctacggg cagaccgccg aaggcctgcc ctttccgcag gccatccgtc
    10021 cggagagcct cgtgcgcatc atcgagacgc tgcccgcggg cgtcaccgtg ctggtgtgcc
    10081 acccggccga gcgcgcggac ctggagacga cctacctgca cgagcgccgg atcgagctgg
    10141 cgacgctgtg cgaccgacgc gtgcgcgccg cgctcgcgtc gcgcggcgtg gcgctgcgct
    10201 cgttcgagga aagcgcagcg gagacgaaaa gcccggaccg ggcggtccgg gcccgtgcgg
    10261 cgcgcgcgct ctaggcgacc agcgccgcct tcagcttcgg atggcgcccg aagaagtcga
    10321 tcttctcggc ccagccgtcc aggccccaga cgtgacccgc gcgctcgacg atgagctgca
    10381 cgagcacgac ggcgtagacg atgtggaagt cgagcaggaa gttgttcttg ttctcgatgt
    10441 acgggaagct catgtgcgcg gtccagtaca ccagcatcag caggatcccg aaggacgcgc
    10501 tcacgcgcac gaacaggccg
//
LOCUS       DATDMW010000141        29898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_913012, whole genome shotgun sequence.
ACCESSION   DATDMW010000141 DATDMW010000000
VERSION     DATDMW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 29898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29898
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913012"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="VI319_06190"
     CDS             complement(<1..1607)
                     /locus_tag="VI319_06190"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEY6863472.1"
                     /translation="MSKKRRKGGDGNTLDLFDDDKKLKGGKGGGGGKPPPARASKGDD
                     DSIDLGAFAERSYLTYAMSVVRGRAIPNVEDGQKPVQRRILYVMGELGLRPGAKPIKS
                     ARIVGEVLGKYHPHGDAATYEAMVRMAQSFTLRYPLIDGQGNFGTADGDSAAAYRYTE
                     ARLTPIADLLLSEIDQDTVDFVPSYDGAFREPELLPARLPMLLLNGASGVAVGMACEF
                     QPHNLREVADAAVQLLQHPRTTTDKLLEDIQGPDFPGGAQIISSRQEIREAYETGRGS
                     LRARCRWKVEELARGQWRVVVTELPPTTSATRVMEEIEAIVDPKAKEKGGKVTQEQQN
                     KKALLLSQLEKVTDESDKNNKVRLVLEPRTSRLDPDEFMKLLLAHTSLEENFPINMVV
                     LGRDGRPGRKNLRDLLEEWVGFRVDTVTRRTKHRLGEVERRIHILDGRKIVYLNIGEV
                     IKIIRASDEPKPALMKRFKLTEVQAEDILEIRLRQLARLEAIKIEQELKEQRDQQSRL
                     EDILSSPASLKRTVVKEIEADAKQHGD"
     gene            complement(1604..3565)
                     /locus_tag="VI319_06195"
     CDS             complement(1604..3565)
                     /locus_tag="VI319_06195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY6863473.1"
                     /translation="MAKKTYDEASITKLKGIEPVKLLPGMYTRVSDPTHIIQEAIDNA
                     ADEAYGGHASRIDVTVHKDGSVTVADNGRGIPVGLHPVEKIPTVQLVFTELHAGAKFY
                     KRSGDAAYQFSGGLHGVGVSVTNALSERLEVEVVREGERHRIVFADGDVKEKLKKVGS
                     AKGSSTTLRIWPNKKYFDSPKVSMPDLERLVRSKAVLLPGVTVTLTVEGSKDGTRTWK
                     YPEGMAGYLNEMLTTGELQAVAPVVTAERYVEEGNGFAKGEGAGWALAFIDGEQKGAG
                     ESYANLIPTPAGGTHESGMRDGAFEAVKSFAEHHNMMPRGIKLLPDDVWSRAVYFLSA
                     KILEPQFHGQTKEKLSNRDAVKLVSGMVKDPLELWMNQHVDHGRRIAEVAIAQAVNRS
                     KAVQKVEKKKGSSVVMLPEKLTDCESSDIAENELFLVEGDSAGGSAKQARDKRFQAIF
                     KMRGKALNSWEVERERLFSNKEIHDIAVALGVDPHGPDDEPSLEGLRYGKIATMTDAD
                     VDGAHISVLLLTLFFKHFPKLIERGHIYVAQPPLYKIDAPAHGKRPAKKVYALDDEEL
                     KGIKDRLADEGVKVDALSVQRFKGLGEMNPDQLWETTLSPDTRRLLPVKVTDAKKTLA
                     MFNMLMSKKEAEARCAWMEEKGDQVEADV"
     gene            3654..4751
                     /locus_tag="VI319_06200"
     CDS             3654..4751
                     /locus_tag="VI319_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863474.1"
                     /translation="MNDSAQPDMLRDPYGSWLQAEGVPVVEDFGIDLLELPVRPWPRF
                     GVDGGVAHLKGRGDFVAVFVLDLAPGAKSEPQRHLYEEVVYVLSGHGSTTIETADGRR
                     HSFEWGPKSLFALPLNARYRHFNASGRERARLASTNNLPIVMNLFHNEAFIFDNPFEF
                     SERLGTARYFAGEGDFIPVRPGRHMWETNFVPDLAAFELKTWEQRGAGSSNMKFILAD
                     GTMHAHSSQMPVGTYKKAHRHGPDFHVYAVTGHGYSLFWYEKAGDFVRIDWKHGTVFA
                     PPDMMFHQHFNTAPEPSRYLAVALGSLRYPFTSEKRTQFSSVDVNVDQGGRQIDYKDQ
                     DPRIHKIFLEELAKHGVKSGMAKHIDESTVA"
     gene            4751..6262
                     /locus_tag="VI319_06205"
     CDS             4751..6262
                     /locus_tag="VI319_06205"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEY6863475.1"
                     /translation="MHDLDFEHVHVDPEERAQLSDAIWSADNVELTTVGIDIGSSTSH
                     LMFARVHMQRLATALSSRFVVVDRKILWESPILLTPYLPDYTIDTEKLAAFVDSCYSG
                     AALERAAVDSGAVILTGEALKRRNARAIADLFSAESGKLVCASAGHHMEAALAAQGSG
                     TMKLSAERRKTLLNVDIGGGTTKLALVRNGELAATVAIAVGGRLLVEDPENGLVRIEE
                     PIVEVARSLGIELSAGKKLAPRERRKIVERMAQILVAMISRREPDALASRLLVTAGWP
                     KPLLDLSIDAITFSGGVAEYVYGRETRRFGDLGPDLAHALAHAIEDGDLRAPIWDPGH
                     GIRATVTGASQFTVQVSGNTIFIAEPDILPLRNLPVAACRIDLGDAVSAPAIERAVRG
                     TLALTDFEDGSGPVAFAFPWTGDPDHARLHAVASGIAAAVPRTVAAGMPVVLMIDGDV
                     GLTLGRVLHREVAPGSRVVAIDSVRLNAFEYVDIGTPVAKTGVVPVIIKSLVF"
     gene            complement(6425..7027)
                     /locus_tag="VI319_06210"
     CDS             complement(6425..7027)
                     /locus_tag="VI319_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEY6863476.1"
                     /translation="MKFRKTIIAATLALAAAWTAPAFAHCDTLDGPVVGAARKALDTG
                     NVNLVLVWVQKNDEAQIRDAFQKARNVRKAGGDAKELADRYFFETLVRIHRAGEGAGY
                     TGLKPAGQVEPPVAAADKAIASGRLEGLAKTISARTEKGLHEQFAQVMAKKKYDANDV
                     AAGRAYSSAYVEFVHYAERLYDAAETMAPEHAQPAPAHNH"
     gene            complement(7131..7817)
                     /gene="narI"
                     /locus_tag="VI319_06215"
     CDS             complement(7131..7817)
                     /gene="narI"
                     /locus_tag="VI319_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEY6863477.1"
                     /translation="MSDFLHQLLFGIYPYVCLAVFLLGSLIRFEREQYTWKSDSSQLL
                     RRGQLRWGSNLFHVGILMLFFGHLVGLLTPPAAYHALGLSTEAKQMLAVGAGGIFGAM
                     CLIGLILLVYRRLAEPRIRATSTRMDIVILLWILATLGLGLASIWWSLGHRDGGVMLQ
                     LAAWAQHIVTFRAGAADFIREVPPIYRVHLFFGMTVFLLFPFSRLVHVWSGFATVGYL
                     GRAYQIVRGR"
     gene            complement(7814..8470)
                     /gene="narJ"
                     /locus_tag="VI319_06220"
     CDS             complement(7814..8470)
                     /gene="narJ"
                     /locus_tag="VI319_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEY6863478.1"
                     /translation="MKTYRALSALLSYPEPALVGALPEISTALDEEELLPRAARRAVA
                     ELVAELAGADLYDLQERYVSLFDRGRALSLHLFEHVHGESRDRGQALVDLANRYRERG
                     YRIAGNELPDYLPLFLEYLSLLPCREAKDQLAETAHIVRAIGDGLVRRGSRYAAVPAA
                     LLALAGEPGLTQKIAAPPGAEDSAEALDRAWADEPVTFGGGCAAPGTRPVRFYRARTA
                     "
     gene            complement(8467..9999)
                     /gene="narH"
                     /locus_tag="VI319_06225"
     CDS             complement(8467..9999)
                     /gene="narH"
                     /locus_tag="VI319_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEY6863479.1"
                     /translation="MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGMEYAWFNNV
                     ETKPGVGYPKDWENQERWSGGWVRSGSGRLQPKQGGKLKVLANIFANPNLPQIDDYYE
                     PYTFDYEHLHDAPRMEAAPVARPRSLITGERMEKVESGPNWEEILGGEFEKRSKDANF
                     EAMQKQMYGEFENTFMMYLPRLCEHCLNPTCVASCPSGSIYKREEDGIVLIDQDKCRG
                     WRMCVSGCPYKKIYYNWESGKAEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVVLYDA
                     DRIEQAASVAEPKDLYEAQLRLFLDPNDPAIVEQARRDGVPDAWLEAAKASPVYKMAM
                     DWKLAFPLHPEYRTLPMVWYVPPLSPISAAANSGLLGANGEIPDVSQLRIPLRYLANL
                     LTAGDEAPVKLALERMLAMRAFMRARHVEKRENAAALAQVGLSAAQVEDMYRTLAIAN
                     YEDRFVIPTTHREHAEDAYDLRGSCGFSFGNGCSGGESGASLFGGKKRVIPLKVLSKA
                     GS"
     gene            complement(9996..13688)
                     /locus_tag="VI319_06230"
     CDS             complement(9996..13688)
                     /locus_tag="VI319_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEY6863480.1"
                     /translation="MSHFLDRLKFLAESREPFADGHGELRREDRGWEDAYRKRWQHDK
                     IVRSTHGVNCTGSCSWKVYVKGGIVTWETQQTDYPRTRPDMPNHEPRGCSRGASYSWY
                     LYSGNRLKYPMVRRPMLELWREARKTSDPVGAWRAIVENPQAAATYKKQRGLGGFLRS
                     SWDEVNEIIAAANVHTIKRWGPDRVIGFSPIPAMSMVSYAAGSRYLSLIGGVCMSFYD
                     WYCDLPPASPMTWGEQTDVPESADWYNSTFIMMWGSNVPQTRTPDAHFLTEARYKGTK
                     VVSVFPDYAEGSKFGDLWLHPKQGTDAALAMAMGHVVLTEYHAKDKSAYFREYCRQYT
                     DMPMLVRLVKQGERYVPERFLRASDFADKLGEANNPEWKTVCFDEASGRLAVPLGSIG
                     FRWGEKGKWNLEEKNPQGAPIKPRLSLAGAKDEVVPVAFPYFGGIAHEHFGSNPQSDV
                     LVRGVPVKKAGEHLVATVYDLLLANYGVGAAKSFDDDVPYTPAWQEAITGVPRDQVIT
                     VARQFAENADKTHGKSMVIIGAGMNHWYHMDMNYRSIINLLTMCGCVGQSGGGWAHYV
                     GQEKLRPQTGWTALAFALDWARPPRQMNSTSFFYAHTDQWRYEKVSVAEILSPLADPK
                     RYTGSLIDFNVRAERMGWLPSAPQLQRNPLEIAKEAARAGVDPKDYVVQQLKSGKLKM
                     SCEDPDHPDNFPRNLFVWRSNLLGSSGKGHEYFLKHLLGTTHGVQGKDLGEMGGQKPE
                     EVAWRETTAEGKLDLLVTLDFRMSTTCLYSDIVLPTASWYEKNDLNTSDMHPFIHPLS
                     AAVDPVWQSKSDWEIYKGIAKKFSEIAAHHLGVEKDVVLTPLMHDTPGELGQAFEPKD
                     WKTGECEPVPGKTLPQITIIERDYPNTYKRFTALGPLMSKIGNGGKGIAWNTQHEVEL
                     LGSLNYEVTEEGPTKGLPKIDSDIDACEVILALAPETNGEVAVKAWNALSEITGREHA
                     HLAVPREDDKIRFRDVQAQPRKIVSSPTWSGVESEHVSYNAGYTNVHELIPWRTLTGR
                     QQLYQDHPWMRAFGETLCVYRPPVDTKTVQPMLGKRANGHPELVLNFITPHQKWGIHS
                     TYTDNLLMLTLSRGGPIVWMSEKDASKAGIVDNDWVEAYNVNGALTARAVVSQRVPEG
                     MVMMYHAQEKIVNTPGSEVTGTRGGIHNSVTRACPKPTHMIGGYAQQSYGFNYYGTVG
                     ANRDEFVIVRKMAKVDWMDAPAKERP"
     gene            complement(13699..15114)
                     /locus_tag="VI319_06235"
     CDS             complement(13699..15114)
                     /locus_tag="VI319_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HEY6863481.1"
                     /translation="MTTQAVPLRSRLGKTLTLWTPEDKEFWITQGRAIANINLWISVP
                     ALFLAFAVWQLWSVVAVNLNALGYRFTPNQLFWLAAAPALSGATLRIFYSFMIPVFGG
                     RRWTAISTASLLVPALGIGVAIQDQTTSYGTFLALALLCGFGGGNFSSSMANINFFFP
                     KERKGSALGVNAGLGNLGVSAVQFMAPLVIAAGVYGPIAGDPQTIVRSGQTVQIWAQN
                     AAFVWVPFIVVASIAAWVGMNDLATAKASVREQAVIFGRKHNWLMCWLYLGTFGSFIG
                     YSAGFPLLIKSQFPEVSALAYAWMGPLVGAAIRPFGGWLADKLGGAIVTFWTFVVMAA
                     GVAGVIYFLPSGPSGGSFAGFFVCFIVLFLMTGIGNGSTFRMIPIIFLTEHQRAAAGK
                     GTDAQEQAVKQATREGAAVLGFTAAIGAYGGFFIPKSYGTSIELTGGPESALVVFIVF
                     YLTCIAVTWWYYARRNAEMPC"
     gene            complement(15241..15984)
                     /locus_tag="VI319_06240"
     CDS             complement(15241..15984)
                     /locus_tag="VI319_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6863482.1"
                     /translation="MPKTNIVLLGLLSVALAGCAGHRTAGAANYAAIVAAPDRSDADR
                     QTDRRRDPATMLLFTRVRPGMKVLDMGAGGGYSTELLARAAGPTGVVYAQDSSDVSPR
                     AKERFEARMKTPPMKNVVRLERPFDDPVPPGVRDLDLVTFFFFYHDTTYMPVDRAKMN
                     RALFDALKPGGVLVIADHSAKPGDGTSVGKSLHRIEEGALVREVEAAGFRLVERGEFL
                     RHPEDPRDTPVFRPKVPVDEFVLKFGKPR"
     gene            16049..16852
                     /locus_tag="VI319_06245"
     CDS             16049..16852
                     /locus_tag="VI319_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY6863483.1"
                     /translation="MERRAFLLGALAAAASPCVIAQETPPGAADAALREKLATCARIF
                     AMQGLLGLFGHVSAFDPKGRRVLITPGAGVDKARLKGPDMVVIDLSGKVLEGRGRPPI
                     EWPIHTALHAARADALAVAHLHPPQATLFGIARREFAPVTVQGCLLIDGVPLYRDAEL
                     VTTIERGQRLAATIGAKPALFMRGHGVVVAGKSLERVLYAALILEDEARKAAQAAALG
                     EVGTIGEEECRAFSTDAEWDARSRRAWSYYAAMESRWDRQPASGMVPFS"
     gene            complement(16860..17756)
                     /locus_tag="VI319_06250"
     CDS             complement(16860..17756)
                     /locus_tag="VI319_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6863484.1"
                     /translation="MDKLRAMTAFTRIVDKGSLTAAAADLGTSLPSVVRTLAALEREL
                     GTTLLQRTTRRLRLTDEGRQYHEHCRIILGQLRDAEAALASRRLAPHGKLTLTASVMF
                     GRRYVAPLASDFARRWPALSVDLLFVDRVVNLIEEGVDVALRIGPLQDSSLVAIPVGQ
                     VRRVVCASPHYLRAHGTPRRPDDVRAHRCVRFTGLAPRSEWPFRVDSRMMSVPITSAM
                     SCNQVDGALDACIAGLGLGSFLSYMTAPAKRARRLRYVLEKFEADPLPVHVVYPHSRI
                     LSANVRAFVDACVHRLRQTTFE"
     gene            17865..19463
                     /locus_tag="VI319_06255"
     CDS             17865..19463
                     /locus_tag="VI319_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863485.1"
                     /translation="MKRQWLAAFFLLLCAAGHAHDADDRPQAGRAQLGKVEFANSCAA
                     AVAGDFNVSVALLHSFWVKDAIEGFNVVLKQDPSCAIAYWGIAMSLQQNPLTGQEPSA
                     DATRLALAGLDKARAIGAKTQRERDYLAAIDLIYRDADKTPFRARRLAYEKAMEALAQ
                     RYPADTEARIFYALSLDMTADLSDKTYANQLKAAAILEPLSRELPEHPGIAHYLIHSY
                     DYPAIASRGVEAARRYAEVAPGHPHALHMPSHIFTRLGTWQASVDTNRRSAEAAKGEG
                     NGGEQAHAMDYMVHAYLQLGQDGQAKRVVDESRTITTLNPASFVGYYGVAAMPARYAL
                     ERRAWKDAVALEPRETRWPFTTAMTHFARGLGFARLGDTASAQKEIDVLASERDALRS
                     QSNAYWANQVEVQRLAVAAWTALAQGARDDALKSMQASADLEDSMEKHIVTPSPVVPA
                     RELLGDMLLELKRPAEALAAYEASAVREPNRLRGIYGAAQAAALGGDRDKARSYYARL
                     AALAAGANPGRPELQQAKAYLAKR"
     gene            complement(19473..19856)
                     /locus_tag="VI319_06260"
     CDS             complement(19473..19856)
                     /locus_tag="VI319_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863486.1"
                     /translation="MVRMVVALLVQAVLVDTALAQGAGGIDPASLADGTQKALVQQYC
                     DKDGRFMRCLGVDIKKDAARCADLVRGNWAFCRRTFMMTAPATVPASDARGYTDNLAD
                     CLRAGAVAAAGKSPGAVDQCMSETR"
     gene            complement(19996..21495)
                     /locus_tag="VI319_06265"
     CDS             complement(19996..21495)
                     /locus_tag="VI319_06265"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY6863487.1"
                     /translation="MSGMLSVVSYGLGTIVGFALIGVLLFWFVQDVTQKKHSVLRNYP
                     VVGRLRYLLEKQGEYFRQYFFMNDREEMPFNRATRGWVYRLAKAEGGVIGFGSTNNTN
                     EPGSILFVNHPYPVLEEDRLPTPPLAIGEGWCDRPFVAKSVVNVSGMSFGAISEPAVR
                     ALSRGAKMAGCWMDTGEGGLSPFHLEGGCDVIMQIGTANYGVRSANGGFSSERARELA
                     RHPEVKAFEIKLSQGAKPGKGGVLPAAKVTEQIAGIRGIPPHQDSISPNRHRDVANVD
                     QLLDKIAYIRDLTGKPVGVKTAIGGWQFINELADAVHRRGLEVAPDFLAIDGGEGGSG
                     AAPQALMDHMALPIAEALPRVVDALIESGLRERIRVIAAGKLVNPARAAWALCVGADF
                     VNTARGFMFSLGCIQALRCHQNTCPTGVTTHNPRLQRGLVVEDKAERVANYAQGMNRE
                     IDMIAHSCGCRHARELRREHVRIVQSAGQSVALNILYPYPEASAKRKAA"
     gene            complement(21530..22009)
                     /locus_tag="VI319_06270"
     CDS             complement(21530..22009)
                     /locus_tag="VI319_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6863488.1"
                     /translation="MKVTKNAVVSLDVELSDIWGNLIQKSDEPVQYLHGGYGNVFPAV
                     EALLEGKSAGERVETRLEPEDAFGDYDENLLRMAPRSDFPEGLEVGMRVEGDDGKGLY
                     TVTDIAEGQVVLDGNHPLAGMALKFVARVADVRPATPAEIENGTADDPESVILRILP"
     gene            complement(22041..22508)
                     /locus_tag="VI319_06275"
     CDS             complement(22041..22508)
                     /locus_tag="VI319_06275"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY6863489.1"
                     /translation="MLLAGQPAPVFSLPDADMESCDLADFRGKKHVVLYFYPKDGTPG
                     CTMQATDFSDHEQDFARYDCIVIGVSPDDCLRHQAFRDEHGVSIRLLADPEGEACRAY
                     GVLVEKETNGTKKPCIVRSTFIIDKRGTVRHALYEVQPKGHAAEVLSLVKSLR"
     gene            22711..23769
                     /locus_tag="VI319_06280"
     CDS             22711..23769
                     /locus_tag="VI319_06280"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863490.1"
                     /translation="MLIRSAIPGFSGIIQPPELFRLAGRDDVESRIVARRAGRWTLAQ
                     GPFSRADIERQPRTNWTLLVQGVNCVHPPADDLLRRFDFIPYARLDDVMVSYAAPGGG
                     VGPHVDSYDVFLLQASGRRRWRLSGKVAPEFREDAPLRVLKRFRPEREWVLEPGDMLY
                     LPPGWAHEGTAVGACTTCSIGFRAPARAELAGEFLAWLGAHADLPGLYRDPGLRPAAN
                     AAEIPRELVRAAARAVRSLRWTERDLAGFVGSYLTAPKPRIVFDPPPRALSPAVFARR
                     CREKGLALDARSQLLFLGRDFFVNGEPFAPRPALAPPLRRLAHERRLGPGRAPAALCA
                     LAYRWYLSGWVRPGDGHG"
     gene            23762..24265
                     /locus_tag="VI319_06285"
     CDS             23762..24265
                     /locus_tag="VI319_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863491.1"
                     /translation="MDDAVREAAPQRRLLATRKEYLEAIEQLLGLVARELRIFDPDLA
                     ELGMNAEPRIARLRAFLAASPTHRLYVAIRDTDFVTRRCARVLALLGSYPRQMFIQQT
                     VGEALKAQDCFVIADDRHLVRRSVAAQPRGVLVLDDPKDCGSIRDRFEEIWESSEPTV
                     SPNVTGL"
     gene            complement(24252..24686)
                     /locus_tag="VI319_06290"
     CDS             complement(24252..24686)
                     /locus_tag="VI319_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863492.1"
                     /translation="MKKPARKAGFFCARAGDAYFFEASDGLDGVAGAELDDGAPEDGA
                     AEDGAPEGALDAGGVDGAVDAGGVEPVLGVDDSFFWQAASARAAAKPAAMRSVRFIVV
                     LLSDRGNGGRRLGQIPTPRVQILRRFIAPRHDPRQIRRVTVP"
     gene            complement(24700..25473)
                     /locus_tag="VI319_06295"
     CDS             complement(24700..25473)
                     /locus_tag="VI319_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6863493.1"
                     /translation="MRFALLGSGSEGNALIVEAGATCLMLDCGFGPRDCVARLERLGL
                     EPSRLAGIVVTHEHSDHADGVFPLARRFGLPVWMTWGTFSAMRWSEPDAGRDVEVRLV
                     DPDSAFAVADLEVRPLTVPHDAREPVQYVFGDGAHRLGVLTDTGCATAHIEASLSGCD
                     ALVLECNHDVDLLMGGEYPPSLKARIAGRWGHLDNAVSAAILAALDRSRLKHVVAAHL
                     SRKNNTPELARAALSSALGCAPGWIAVATQEEGLGWRDV"
     gene            complement(25484..26623)
                     /gene="bamC"
                     /locus_tag="VI319_06300"
     CDS             complement(25484..26623)
                     /gene="bamC"
                     /locus_tag="VI319_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEY6863494.1"
                     /translation="MTRAARRALLAATVTLGLAGCTTGFWEGKKIDYKSAEAQRTTTL
                     DVPPDLTSPTRDDRYQVPDVSPTGTATFSAYSSERAGAARTASPNDVLPEVKDAHVER
                     AGAQRWLVVQMPPERVWPVVKEFWQEAGFLIKSENPEIGVMETDWAENRAKIPDDIVR
                     NILGKVIDGMYSTSERDKFRTRLERGAQPGTTEVYISHRGVVEVYTNQQRDSTVWQPR
                     PSDPELEAEFLRRLMVRFGVDEARAKQQVAEGPKQERARIVTMQDGTGSLELSEPFDR
                     AWRRVGLALDRVGFTVEDRDRAHGYYFVRYVNPEKDALDTDKQGFFSRLIFGKNKGIG
                     STEQYRVQVKDLKAISEVQVLNKDGATEPSDTARKILALLHDQLK"
     gene            complement(26620..27546)
                     /gene="dapA"
                     /locus_tag="VI319_06305"
     CDS             complement(26620..27546)
                     /gene="dapA"
                     /locus_tag="VI319_06305"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY6863495.1"
                     /translation="MTKPSLPRGSIVAIVTPMHEDGRLDLPRFRALIDWHIAEGTDGI
                     VVVGTTGESPTVDFDEHKELIKIAVEHSRGRIPIIAGTGGNSTAEAIELTESAKKAGA
                     NACLSVVPYYNKPTQEGMYRHFRAAAERVDIPQIVYNVPGRTVADLANDTTLRLAQVP
                     NIVGIKDATANIERGSDLLRRAPKGFMVWSGEDATGLALILLGGSGIISVTANVAPRL
                     MHEMCAAGLQEDVKRARELNFKLLGLHQKLFVEANPIPVKWAAAQMGLIGPGIRLPLT
                     PLSPQFHDVVREAMRQADLAPAGLRVVEGRTA"
     gene            complement(27559..28095)
                     /locus_tag="VI319_06310"
     CDS             complement(27559..28095)
                     /locus_tag="VI319_06310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6863496.1"
                     /translation="MSHDLESPSDWVRRWAHLVRPGGRVLDLACGGGRHARFLAGLGL
                     RVLGVDRDPAALAGVPGVELRVADLEGGPWPLGDERFDGVVVTNYLHRPLFPRLVDAL
                     APGGVLIYETFALGNERYGRPGNPDFLLQPNELLERTRALRPVAFEQGVVARPKPAVV
                     QRLCAVRESEWPPELEPR"
     gene            28142..29362
                     /locus_tag="VI319_06315"
     CDS             28142..29362
                     /locus_tag="VI319_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/OsmC family
                     protein"
                     /protein_id="HEY6863497.1"
                     /translation="MAPSSKPITFPGASGAMLAARLDGPLAPPLAYVLFAHCFTCSKD
                     TKGAVYISEALAEAGLAVLRFDFTGLGGSEGDFANTNFSSNVADLVAAADWLRREHKA
                     PAILVGHSLGGAAMIAAAAKIPEARALVTIGSPAEPAHLGHLLRDSRETIERTGEATI
                     DIGGRPFHVKKQLLEDIESQKIAEALPRLGKALLVMHSPKDTVVGIDNASKIFLAAKH
                     PKSFVSLDDADHLLGRRADARYAAGVLAAWSQRYLGVEPEKVPEAPEGRVLVRETREG
                     KFTNQVVVGRHVFRADEPVAVGGNDTGPSPYDLLCAALGACTAMTIRSYADLKKLPLE
                     RASVELRHDKIYAADCADCETREGKIDRIERVVRLEGDLNPEQRAKLLEISNKCPVHR
                     TLHSEISIVTALGD"
     gene            complement(29407..>29898)
                     /locus_tag="VI319_06320"
     CDS             complement(29407..>29898)
                     /locus_tag="VI319_06320"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY6863498.1"
                     /translation="WFTKSDDFDLEIRERFSSLWTKASGGALASWRGTPLAALALVVV
                     LDQFPRNMFRGEPRAFASDALALATAEDMIERRFDRLLRPIERAFVYLPFQHAEDVEI
                     QRRSVALYDELAILGVDHRDFARRHHDIVSRFGRFPHRNEILGRESTREEIEFLKQPG
                     SRF"
BASE COUNT         4462 a        10392 c        10207 g         4837 t
ORIGIN      
        1 tcgtcgccgt gctgcttggc gtcggcctcg atctccttga ccacggtgcg cttgagcgag
       61 gccgggctgg agaggatgtc ctccagcctg ctttgctgat cgcgctgctc cttcagctcc
      121 tgctcgatct tgatcgcttc cagccgcgcc aactggcgca gccggatctc gaggatgtcc
      181 tcggcctgga cctcggtgag cttgaagcgc ttcatcagcg cgggcttggg ctcgtcggag
      241 gcgcggatga tcttgatcac ctccccgatg ttcaggtaga cgatcttgcg gccgtcgagg
      301 atgtggatgc gccgctcgac ctcgcccagg cggtgcttcg tgcgccgcgt gacggtgtcg
      361 acccggaagc cgacccactc ctcgagcagg tcgcgcaggt tcttgcggcc gggccggccg
      421 tcccggccga gcaccaccat gttgatgggg aagttctcct cgaggctggt gtgcgcgagg
      481 agcagcttca tgaactcgtc gggatcgagg cgcgacgtgc gcggctcgag caccagccgc
      541 accttgttgt tcttgtcgga ctcgtcggtg accttctcga gctgcgagag cagcagcgcc
      601 ttcttgttct gctgctcctg cgtgaccttg ccgcctttct ccttcgcctt cggatcgacg
      661 atcgcctcga tctcctccat gacgcgcgtc gccgaggtcg tcggcggcag ctcggtcacg
      721 accacgcgcc actggccgcg cgcgagctcc tcgaccttcc agcggcagcg cgcgcgcagc
      781 gagccgcgcc cggtctcgta ggcctcgcgg atctcctggc gcgaagagat gatctgcgcg
      841 ccgccgggga agtcggggcc ctggatgtcc tcgagcagct tgtcggtcgt cgtgcgcggg
      901 tgctgcagca gctgcaccgc ggcgtccgcc acctcgcgca gattgtgcgg ctggaactcg
      961 cacgccatgc cgaccgcgac gccggaagcg ccgttcagca gcagcatcgg cagccgcgcg
     1021 ggcagcagct cgggctcgcg gaacgcgccg tcgtagctcg ggacgaagtc gaccgtgtcc
     1081 tggtcgatct ctgagagcag cagatcggcg atcggcgtga gccgcgcctc cgtataccgg
     1141 taggccgcgg ccgagtcgcc gtccgcggtg ccgaagttgc cctggccgtc gatcagcggg
     1201 tagcgcagcg tgaagctctg cgccatccgc accatcgcct cgtaggtggc ggcgtcgccg
     1261 tgcgggtggt acttgccgag cacctcgccg acgatgcgcg ccgacttgat gggcttcgcg
     1321 cccgggcgca ggcccagctc gcccatcacg tagaggatgc ggcgctgcac gggcttctgc
     1381 ccgtcctcga cgttcgggat ggcgcggccg cgcaccacgc tcatcgcgta ggtgaggtac
     1441 gagcgctcgg cgaaggcgcc gaggtcgatc gagtcgtcgt cgcctttcga ggcacgcgcg
     1501 ggaggcggct tgccgccccc tccgcctttg ccgcccttca actttttgtc gtcgtcgaac
     1561 aggtcgagag tattgccgtc gccgcctttg cggcgcttct tgctcatacg tcggcctcga
     1621 cctggtcgcc cttctcctcc atccaggcgc agcgcgcttc ggcttccttc ttcgacatga
     1681 gcatgttgaa catcgcgagc gtcttcttcg cgtcggtgac tttaacgggc agcagccgcc
     1741 gcgtatcggg cgagagcgtc gtctcccaca gctggtcggg gttcatctcg cccaggccct
     1801 tgaagcgctg caccgacagc gcatcgacct tcacgccttc gtcggcgagc cggtccttga
     1861 tgcccttcag ctcctcgtcg tcgagcgcgt aaaccttctt cgcgggacgc ttgccgtggg
     1921 ccggcgcgtc gatcttgtac agcggcggct gcgcgacgta gatatggcca cgctcgatga
     1981 gcttcggaaa gtgcttgaag aagagcgtga ggagcagaac cgagatatgg gcgccgtcga
     2041 cgtcggcatc cgtcatcgtg gcgatcttcc cgtagcgcag gccctcgagg ctcggctcgt
     2101 cgtcgggccc gtgcgggtcg accccgagcg cgaccgcgat gtcgtggatc tccttgttcg
     2161 agaacaggcg ctcgcgctcg acttcccagg agttgagcgc cttgccgcgc atcttgaaga
     2221 tcgcctggaa gcgcttgtcg cgcgcctgct tggccgagcc gccggccgag tcgccctcga
     2281 ccaggaacag ctcgttctcc gcgatgtcgc tcgactcgca gtcggtgagc ttctccggga
     2341 gcatcacgac cgacgagccc ttcttcttct cgaccttctg cacggccttc gagcggttca
     2401 ccgcctgcgc gatcgcgacc tcggcgattc tccggccgtg gtcgacgtgc tggttcatcc
     2461 agagctcgag cgggtccttg accatgccgg agacgagctt caccgcgtcg cggttggaga
     2521 gcttctcctt ggtctgcccg tgaaactgcg gctcgaggat cttggccgag aggaagtaca
     2581 ccgcgcgcga ccagacgtcg tcgggcagca gcttgatccc gcgcggcatc atgttgtggt
     2641 gctcggcgaa ggacttcacc gcctcgaacg cgccgtcgcg catccccgac tcgtgcgtgc
     2701 cgccggcggg cgtcgggatc aggttcgcgt acgactcgcc ggcgcccttc tgctccccgt
     2761 cgatgaaggc gagcgcccag ccggcgccct cgcccttcgc gaagccgttg ccctcctcga
     2821 cgtagcgctc ggcggtgacg acgggcgcga ccgcctgcag ctcgcccgtc gtgagcatct
     2881 cgttgagata gcccgccatg ccctcgggat acttccacgt gcgcgtgccg tccttcgagc
     2941 cttcgacggt gagcgtcacg gtgacgccgg gcagcagcac cgccttcgag cgcaccagcc
     3001 gctcgagatc gggcatcgag accttgggcg agtcgaagta cttcttgttc ggccagatgc
     3061 gaagcgtggt gctcgagccc ttggccgagc cgaccttctt cagcttctcc ttgacgtcgc
     3121 cgtcggcgaa gacgatgcgg tggcgctcgc cctcgcgcac gacctcgacc tcgagccgct
     3181 cggagagcgc gttcgtgacc gacacgccga cgccgtgcaa ccctccggag aactggtacg
     3241 ccgcgtcgcc cgagcgcttg tagaacttcg cgcccgcgtg cagctcggtg aagacgagct
     3301 gcaccgtcgg gatcttttcc accgggtgca ggccgacggg gatgccgcgg ccgttgtcgg
     3361 ccactgtcac cgagccgtcc ttgtgcaccg tgacgtcgat gcgcgacgcg tgcccgccgt
     3421 aagcctcgtc ggcggcgttg tcgatcgctt cctggatgat gtgggtggga tcgctgaccc
     3481 gggtgtacat cccggggagg agcttgacgg gctcgatgcc cttcagcttg gtgatggagg
     3541 cttcgtcgta cgtctttttc gccatggtcc tcgctgtcgt tgcgctgctg caggcgccat
     3601 tctacatcgg tatcattccg actccgatcc cagcgcaact cgaggtccgc tccatgaacg
     3661 actccgccca gcccgacatg ctccgcgatc cgtacggctc atggctccag gccgaaggcg
     3721 tgcccgtcgt cgaggacttc ggcatcgatc tgctcgagct gccggtgagg ccgtggccgc
     3781 gcttcggcgt ggacggcggc gtcgcgcatc tgaaaggccg cggcgacttc gtcgcggtgt
     3841 tcgtgctcga cctcgcgccc ggcgcgaagt cggagccgca gcgccacctc tacgaggagg
     3901 tcgtgtacgt gctctcgggc cacggcagca cgacgatcga gaccgccgac ggccgcaggc
     3961 actcgttcga atggggcccg aagagcctgt tcgcgctgcc gctcaacgcg cgctaccggc
     4021 atttcaacgc gagcggccgc gagcgcgcgc gtctcgcctc gacgaacaac ctgccgatcg
     4081 tgatgaacct gttccacaac gaggcgttca tcttcgacaa tcccttcgag tttagcgagc
     4141 gcctcggcac cgcccgctat ttcgccggcg agggcgactt cattcccgtg cgtcccggcc
     4201 gccacatgtg ggagacgaac ttcgtccccg acctcgccgc cttcgagctg aagacctggg
     4261 agcagcgcgg cgcgggcagc tcgaacatga agttcatcct cgccgacggc accatgcacg
     4321 cgcactcgtc gcagatgccg gtcggcacct acaagaaggc gcaccggcac ggcccggact
     4381 tccacgtcta cgccgtcacc ggccacggct actcgctctt ctggtacgag aaggccgggg
     4441 acttcgtgcg catcgactgg aagcacggca ccgtgttcgc gccgcccgac atgatgttcc
     4501 accagcactt caacaccgcg cccgagccct cgcgctacct cgcggtggcg ctcggcagcc
     4561 tgcgctaccc cttcacctcc gagaagcgca cgcagttctc gagcgtcgac gtcaacgtcg
     4621 accagggcgg gcgccagatc gactacaagg accaggaccc gcgcatccac aagatctttc
     4681 tcgaggagct cgcgaagcac ggcgtgaagt cggggatggc gaagcacatc gacgaatcga
     4741 cggtcgcata gtgcacgacc tcgacttcga gcacgtccac gtcgaccccg aggagcgggc
     4801 gcagctctcc gacgcgatct ggagcgcgga caacgtcgag ctgaccacgg tcggcatcga
     4861 catcggctcc tcgacgtcgc acctgatgtt cgcgcgcgtg cacatgcagc gcctcgccac
     4921 ggcgctgtcg agccgcttcg tggtggtcga ccggaagatc ctctgggagt cgccgatcct
     4981 cctgacccct tatcttcccg attacacgat cgatacggag aagctcgccg cgttcgtcga
     5041 ctcctgctat tcgggcgctg cgctcgagcg cgccgcggtc gactccggcg ccgtgatcct
     5101 caccggcgag gcgctcaagc gccggaacgc gcgcgcgatc gccgatctct tctccgccga
     5161 gtcgggcaag ctcgtgtgcg cctccgcggg ccaccacatg gaggcggcgc tcgcggcgca
     5221 agggtccggg acgatgaagc tctcggccga gcgcaggaag acgctgctca acgtcgatat
     5281 cggcggcggc accacgaaac tcgcgctggt gcgcaacggc gagctcgccg cgacggtcgc
     5341 gatcgcggtc ggcggacggc tgctggtcga ggacccggag aacggactcg tccgcatcga
     5401 agagccgatc gtggaggtgg cgcgctcgct cgggatcgag ctttccgcgg gaaagaagct
     5461 tgctccgcgc gagcgccgga agatcgtcga gcggatggcg cagattctcg tcgcgatgat
     5521 ctcgcgtcgg gagcccgatg cgctggcgtc gcggctcctg gtgaccgcgg gctggccgaa
     5581 gcctctcctc gacctgagca tcgacgcgat caccttctcg ggcggcgtcg ccgaatacgt
     5641 ctacggccgc gagacgcggc gcttcggcga tctcggcccc gacctcgcgc acgcgcttgc
     5701 gcatgcgatc gaggacggcg atctgcgcgc gccgatctgg gacccgggac acggcatccg
     5761 cgcgacggtg acgggcgcgt cgcagttcac cgtgcaggtc agcggcaaca cgatcttcat
     5821 cgccgagccg gacatcctgc cgctgcgcaa ccttccggtc gcggcctgcc gcatcgattt
     5881 gggcgatgcc gtctccgcgc cggcgatcga gcgcgccgtc cgcggcacgc tggcgctgac
     5941 tgatttcgag gacggctcgg gtcccgtcgc cttcgcattt ccctggaccg gcgatcccga
     6001 ccacgcgcgg ctgcacgccg tcgcctcggg catcgcggcc gcggtgccgc gcaccgtcgc
     6061 cgcgggaatg ccggtcgtcc tgatgatcga cggcgacgtc ggcctcacgc tcggccgcgt
     6121 cctgcaccgc gaggtggcgc cggggagccg cgtcgtcgcc atcgacagcg tcaggctcaa
     6181 cgctttcgaa tacgtcgaca tcggaacgcc ggtcgcgaag accggcgtcg tgccggtgat
     6241 catcaagtcg ctggtgttct gagcgccttc tccagcgact cgcgcacgcg ctgcccgacc
     6301 acgatggcgg ccggatagag cacccgctcc tccgcgggag ctcggcttcc agccgcttcg
     6361 tcatcggcag cgcctcggcc atctccgcgg tgacgcggcc gacagaacct catggcgtgc
     6421 ctcctcagtg gttgtgggcg ggagccggct gcgcgtgctc gggcgccatc gtctcggcgg
     6481 cgtcgtagag ccgctcggcg tagtgcacga actcgacgta cgcgctcgag taggcgcgcc
     6541 cggcggcgac gtcgttcgcg tcgtacttct tcttcgccat cacctgcgcg aactgctcgt
     6601 gcaagccctt ctccgtgcgc gccgagatcg tcttcgccag gccctcgagc ctgccgctcg
     6661 cgatcgcctt gtccgccgct gcgaccggcg gctcgacctg ccccgcgggc ttgaggcccg
     6721 tgtagccggc gccctcgccc gcgcggtgga tgcgcaccag cgtctcgaag aagtagcggt
     6781 cggcgagctc cttcgcgtcg cccccggcct tgcgcacgtt gcgcgccttc tggaaggcgt
     6841 cgcggatctg cgcctcgtcg ttcttctgca cccagaccag cacgaggttg acgttgccgg
     6901 tgtcgagcgc cttgcgcgcc gcgccgacga ccggcccgtc gagcgtgtcg cagtgcgcga
     6961 acgcgggagc ggtccaggcg gcggcgagcg cgagcgttgc tgcgatgatc gtcttgcgga
     7021 atttcatgat cggcctccga taagagaatg gagccatcgt aggaggcggc gggcgtcttg
     7081 ttaacgccgc cgtcggaaaa ccttgacgat tctgccggaa cggcaccgcg ctagcgtccg
     7141 cggacgatct ggtaggcgcg gccgagatag ccgaccgtcg cgaacccgct ccacacgtga
     7201 accaggcgcg agaacgggaa cagcaggaac accgtcatgc cgaagaagag gtgcacgcgg
     7261 tagatcggtg gcacctcgcg gatgaagtcc gccgcaccgg cgcggaacgt gacgatgtgc
     7321 tgcgcccagg ccgcgagctg cagcatgacg ccgccgtcgc ggtggccgag cgaccaccag
     7381 atcgacgcga ggccgaggcc gagcgtcgcc aggatccaca gcaggatcac gatgtccatg
     7441 cgcgtcgagg tcgcgcggat gcgcggctcg gcgaggcgcc ggtagacgag caggatcagg
     7501 ccgatcaggc acatggcgcc gaagatcccg cccgctccga ccgcgagcat ctgcttcgcc
     7561 tcggtcgaca ggccgagcgc gtggtaagcc gcgggcggcg tgagcagccc gacgaggtgc
     7621 ccgaagaaca gcatcaggat gccgacgtgg aacaggttcg atccccagcg cagctgcccg
     7681 cgccgcagga gctggctcga gtcgctcttc caggtgtact gctcgcgctc gaagcggatc
     7741 agcgacccca gcaggaacac ggcgaggcac acgtaggggt agatgccgaa caggagctgg
     7801 tgaaggaagt cgctcatgcg gtcctcgcgc ggtagaaacg caccggccgc gtcccgggtg
     7861 cggcgcagcc gccgccgaac gtgaccggct cgtccgccca ggcgcggtcg agcgcctcgg
     7921 cggagtcctc cgcgccgggc ggcgccgcga tcttctgcgt cagtccgggc tcgcccgcca
     7981 gggcgagcag cgccgcgggc acggcggcgt agcggctgcc ccggcgcacg aggccgtcgc
     8041 cgatcgcgcg cacgatgtgc gccgtctcgg cgagctggtc cttcgcctcg cggcacggaa
     8101 ggagcgacag gtactccagg aacagcggca ggtaatccgg gagctcgttg cccgcgatgc
     8161 ggtagccgcg ctcgcggtag cggttcgcga gatcgaccag cgcctggccg cggtcgcgcg
     8221 actcgccgtg gacgtgctcg aacaggtgca gcgagagcgc gcgtccgcgg tcgaacagcg
     8281 acacgtagcg ctcctgcagg tcgtagaggt ccgcgccggc cagctcggcg acgagctccg
     8341 ccacggcgcg ccgtgcggcg cgcggcagca gctcctcctc gtccagcgcg gtggagattt
     8401 ccggcagcgc gccgacgagc gccggttccg gatacgagag cagcgcggac agagcgcggt
     8461 aggtcttcat gaccccgcct tgctcaggac cttcagcggg atcacccgct tcttcccgcc
     8521 gaacaggctc gccccggact ctccgcccga gcagccgttg ccgaaggaga agccgcacga
     8581 gccgcgcagg tcgtaagcgt cctcggcgtg ctcgcggtgc gtggtcggga tcacgaagcg
     8641 gtcctcgtag ttggcgatcg ccagcgtgcg gtacatgtcc tcgacctgcg cggccgagag
     8701 gccgacctgc gcgagcgcgg ccgcgttctc gcgcttctcg acgtggcggg cgcgcatgaa
     8761 ggcgcgcatc gcgagcatgc gctccagcgc gagcttcacc ggcgcctcgt cgcccgcggt
     8821 cagcaggttg gcgagatacc tgagcgggat ccggagctgc gacacgtccg ggatctcgcc
     8881 gttcgcgccg agcagcccgc tgttcgcggc ggcgctgatc ggcgagagcg gcggcacgta
     8941 ccagaccatc ggcagcgtgc ggtactcggg gtgcagcggg aaggccagct tccagtccat
     9001 cgccatcttg tatacgggcg aggccttcgc cgcctcgagc caggcgtcgg gcacgccgtc
     9061 gcggcgcgcc tgctcgacga tcgccgggtc gttcgggtcg aggaacagcc gcagctgcgc
     9121 ctcgtacaga tccttcggct ccgcgacgga ggccgcctgc tcgatgcggt cggcgtcgta
     9181 gagcaccacg ccgaggtagc ggatgcgccc gacgcaggtc tcggagcaca ccgtcggctg
     9241 gccggcttcg atccgcgggt agcagaagat gcacttctcg gccttgccgc tttcccagtt
     9301 gtagtagatc ttcttgtacg ggcagcccga gacgcacatg cgccagccgc ggcacttgtc
     9361 ctggtcgatg aggacgatgc cgtcctcctc gcgcttgtag atcgaccccg agggacagct
     9421 cgcgacgcag gtcgggttga gacagtgctc gcacaggcgc ggcaggtaca tcatgaacgt
     9481 gttctcgaac tcgccgtaca tctgcttctg catcgcctcg aagttcgcgt ccttcgagcg
     9541 cttctcgaac tcgccgccga ggatctcctc ccagttcggc ccgctctcga ccttctccat
     9601 gcgctcgccg gtgatcagcg agcgcggccg cgcgaccggc gcggcctcca tcctcggcgc
     9661 gtcgtgcagg tgctcgtagt cgaacgtgta gggctcgtag tagtcgtcga tctgcggcag
     9721 gttcggattg gcgaagatgt tcgcgagcac cttgagcttg ccgccctgct tcggctgcag
     9781 cctgccgctg ccgctcctga cccagccgcc gctccagcgc tcctggttct cccagtcttt
     9841 cgggtagccg acgccgggct tggtctcgac gttgttgaac cacgcgtatt ccatccctgg
     9901 acgcgaggtc cagacgttct tgcacgtcac cgagcaggtg tggcagccga tgcacttgtc
     9961 caggttcagg accatgccga tctgcgcgcg gatcttcatg gccgctcctt cgccggcgcg
    10021 tccatccagt cgaccttcgc catcttgcgc acgatgacga actcgtcccg gttcgcgccg
    10081 accgtgccgt agtagttgaa cccgtagctc tgctgcgcgt agccgccgat catgtgggtc
    10141 ggcttcgggc aggcgcgcgt gaccgagttg tggatgccgc cccgggtgcc ggtgacctcg
    10201 gagcccggcg tgttcacgat cttctcctgc gcgtggtaca tcatcaccat gccctcgggc
    10261 acgcgctggc tcaccacggc gcgcgcggtg agcgcgccgt tcacgttgta ggcctcgacc
    10321 cagtcgttgt cgacgatgcc ggccttgctc gcgtccttct ccgacatcca cacgatcgga
    10381 ccgccgcgcg agagcgtcag catcagcagg ttgtcggtgt aggtcgagtg gatgccccac
    10441 ttctggtgcg gcgtgatgaa gttgagcacc agctcgggat ggccgttcgc gcgcttgccc
    10501 agcatgggct ggaccgtctt cgtgtccacc ggcggccggt agacgcacag ggtctcgccg
    10561 aacgcgcgca tccacgggtg atcctggtag agctgctggc ggccggtgag cgtgcgccag
    10621 ggaatcagct cgtgcacgtt ggtgtagccg gcgttgtagg agacatgctc cgactcgacg
    10681 ccggaccagg tgggcgagga gacgatcttg cgcggctgcg cctgcacgtc gcggaagcgg
    10741 atcttgtcgt cctcgcgcgg caccgcgagg tgcgcgtgct cgcgcccggt gatctcggag
    10801 agcgcgttcc acgccttcac cgcgacctcg ccgttcgttt ccggggcgag cgcgaggatc
    10861 acctcgcacg cgtcgatgtc cgagtcgatc ttcggcaggc ccttcgtcgg cccctcctcg
    10921 gtcacctcgt agttcaacga cccgagcagc tcgacctcgt gctgcgtgtt ccaggcgatg
    10981 cccttgccgc cgttgccgat cttggacatg agcggcccca gcgcggtgaa gcgcttgtac
    11041 gtgttcgggt agtcgcgctc gatgatcgtg atttgcggca gggtctttcc gggcaccggc
    11101 tcgcattcgc ccgtcttcca gtccttgggc tcgaacgcct gcccgagctc gcccggcgtg
    11161 tcgtgcatga gcggcgtcag cacgacgtcc ttctcgacgc cgagatggtg cgcggcgatc
    11221 tcggagaact tcttcgcgat gcctttgtag atctcccagt cgctcttcga ctgccagacc
    11281 gggtccacgg cggcggagag cgggtggatg aacgggtgca tgtccgaggt gttgaggtcg
    11341 ttcttctcgt accagctcgc ggtcggcagc acgatgtcgg agtacaggca ggtcgtgctc
    11401 atgcggaagt cgagcgtgac gagcaggtcg agcttgccct cggcggtcgt ttcgcgccag
    11461 gcgacttcct cgggcttctg cccgcccatc tcgccgagat ccttgccctg cacgccgtgc
    11521 gtcgtgccca ggagatgttt caggaagtac tcgtgcccct tgcccgagga gccgagcagg
    11581 ttcgagcgcc acacgaagag attcctcggg aaattgtccg gatggtccgg atcctcgcag
    11641 ctcatcttga gcttgccgct cttcaattgc tgcacgacgt agtccttcgg gtcgaccccg
    11701 gcgcgcgccg cctccttcgc gatctccagc gggttcctct gcagctgcgg cgcggagggc
    11761 agccagccca tgcgctcggc gcgcacgttg aaatcgatga ggctccccgt gtagcgcttc
    11821 gggtccgcca gcggcgaaag gatctcggcg accgacacct tctcgtagcg ccactggtcg
    11881 gtgtgggcgt agaagaacga ggtcgagttc atctggcgcg gcgggcgcgc ccagtcgagc
    11941 gcgaaggcga gcgcggtcca gccggtctgc ggcctgagct tctcctgccc gacgtagtgc
    12001 gcccagccgc cgcccgactg gccgacgcag ccgcacatcg tcagcaggtt gatgatcgag
    12061 cggtaattca tgtccatgtg gtaccagtgg ttcatcccgg cgccgatgat caccatggac
    12121 tttccgtgag tcttgtcggc gttctcggcg aactggcgcg cgaccgtgat cacctggtcg
    12181 cgcgggacgc ccgtgatcgc ctcctgccac gccggcgtgt acggcacgtc gtcgtcgaag
    12241 gacttcgccg cgcccacgcc gtagttggcg aggagcaggt cgtacacggt ggcgacgagg
    12301 tgctcgccgg ccttcttcac ggggacgccg cggacgagga cgtcgctctg cgggttcgac
    12361 ccgaagtgct cgtgcgcgat gccgccgaaa tagggaaacg cgaccggcac gacctcgtcc
    12421 ttcgcgccgg cgagagacag gcgcggcttg atgggcgctc cttgcgggtt cttctcctcg
    12481 aggttccact tgcccttctc gccccagcgg aagccgatcg agccgagcgg cacggcgagc
    12541 ctgccgctcg cctcgtcgaa gcacaccgtc ttccactcgg ggttgttggc ctcgcccagc
    12601 ttgtcggcga agtccgacgc cctgaggaat ctctcgggga catagcgctc gccctgcttc
    12661 accagccgca ccagcatggg catgtcggtg tactgacggc agtactcgcg gaaatatgcg
    12721 ctcttgtcct tcgcgtggta ctcggtgagg acgacgtgcc ccatcgccat cgcgagcgcg
    12781 gcgtcggtcc cctgcttcgg gtgcagccag aggtcgccga acttgctgcc ttcggcgtag
    12841 tcggggaaga ccgagacgac cttcgtgccc ttgtagcgcg cctcggtcag gaagtgcgcg
    12901 tcgggcgtgc gcgtctgcgg cacgttcgag ccccacatca tgatgaaggt cgagttgtac
    12961 cagtcggccg actccggcac gtcggtctgc tcgccccagg tcatcggcga cgccggcggc
    13021 aggtcgcagt accagtcgta gaagctcatg cacacgccgc cgatgagcga gaggtagcgg
    13081 ctgcccgcgg cgtaggagac catcgacatc gccgggatgg gcgagaagcc gatgacgcgg
    13141 tccgggcccc agcgcttgat cgtgtggacg ttcgcagcgg cgatgatctc gttcacctcg
    13201 tcccaggacg agcggagaaa acccccgagg ccgcgctgct tcttgtaggt cgcggcggcc
    13261 tgcgggttct cgacgatcgc gcgccacgcc ccgaccgggt cgctggtctt cctcgcctcg
    13321 cgccagagct cgagcatcgg gcgcctcacc atcgggtact tgaggcggtt tcccgagtac
    13381 aggtaccagg agtaggacgc gccgcgcgag cagccgcgcg gctcgtggtt gggcatgtcg
    13441 ggacgggtgc gcgggtagtc ggtctgctgc gtctcccagg tgacgatgcc gcccttcacg
    13501 tacaccttcc acgagcacga gccggtgcag ttcaccccgt gcgtcgagcg cacgatcttg
    13561 tcgtgctgcc agcgcttgcg gtaggcgtcc tcccagccgc ggtcctcgcg gcgcagctcg
    13621 ccgtggccgt ccgcgaacgg ctcgcgcgac tcggcgagga atttcaggcg gtcgaggaag
    13681 tggctcatgg gatggcgctc agcacggcat ctccgcgttc cggcgggcgt agtaccacca
    13741 ggtcaccgcg atgcaggtca gatagaacac gatgaagacc acgagggccg actcggggcc
    13801 gcccgtcagc tcgatcgacg tgccgtagct cttcggaatg aagaacccgc cgtacgcgcc
    13861 gatcgcggcg gtgaatccca gcaccgcggc gccttcgcgc gtcgcctgct tgaccgcctg
    13921 ctcctgggca tccgtgcctt tgccggccgc ggcgcgctga tgctcggtga ggaagatgat
    13981 cgggatcatg cggaaggtcg agccgttgcc gatgccggtc atgaggaaca gcacgatgaa
    14041 gcacacgaag aatccggcga agctgccgcc cgacgggccc gacgggagga agtagatgac
    14101 gccggcgacg ccggccgcca tgacgacgaa ggtccagaac gtgacgatgg cgccgcccag
    14161 cttgtccgcc agccagccgc cgaagggacg gatcgccgcg ccgacgagcg gacccatcca
    14221 tgcgtacgcg agcgcgctca cctcggggaa ctggctcttg atcagcagcg ggaatcccgc
    14281 cgagtagccg atgaacgagc cgaaggtccc gaggtagagc cagcacatca gccagttgtg
    14341 cttgcgcccg aagatcaccg cctgctcgcg caccgaggcc ttcgcggtgg cgaggtcgtt
    14401 catgccgacc caggccgcga tgctcgcgac gacgatgaac ggcacccaca cgaaggcggc
    14461 gttctgcgcc cagatctgca cggtctggcc cgacctcacg atcgtctgcg gatcgccggc
    14521 gatcgggccg tagacgcccg cggcgatgac cagcggcgcc atgaactgga ccgccgagac
    14581 gccgaggttg ccgagcccgg cgttcacgcc gagcgccgag cccttgcgct ccttgggaaa
    14641 gaagaaattg atgttggcca tcgacgagga aaagttgccg ccgccgaatc cgcacaggag
    14701 cgccagcgcg aggaaggttc cgtagctcgt cgtctgatcc tggatcgcga ccccgatccc
    14761 gagcgcggga acgagcagcg acgcggtgga gatcgccgtc cagcgccggc cgccgaagac
    14821 cgggatcatg aacgaataga agatgcgcag cgtcgcgccg gagagcgccg gcgcggcggc
    14881 gagccagaac agctggttcg gcgtgaaccg gtagccgagc gcgttcaggt tcaccgcgac
    14941 cacgctccag agctgccaca cggcgaacgc gaggaacagc gccggcaccg agatccacag
    15001 gttgatgttg gcgatcgcgc ggccctgcgt gatccagaac tccttgtcct ccggagtcca
    15061 cagggtgagc gtcttgccca ggcgtgacct gagcggaacg gcttgggtcg tcatggcttc
    15121 ctcgcttcct ggcggagcga atgcaccgtc tgccggcgcg cgcgcggcga agcggcattc
    15181 acgaggaaac tgtgccaccc gcggccggcg ggtggttgat ctacgtcaac gcgattcgcg
    15241 tcagcgcggc ttcccgaact tcagcacgaa ctcgtcgacg ggaaccttcg ggcggaagac
    15301 cggcgtatcg cgcgggtcct cgggatggcg caggaactcg ccgcgctcga ccaggcggaa
    15361 tccggctgct tccacctcgc gcacgagtgc tccttcctcg atgcggtgca ggctcttgcc
    15421 cacgctcgtg ccgtcgccgg gcttcgccga atggtcggcg atcaccagca ccccgcccgg
    15481 cttgagcgcg tcgaagagcg cgcggttcat cttcgcccgg tcgaccggca tgtacgtggt
    15541 gtcgtggtag aagaagaaga acgtgaccag gtccaggtcg cgaacgccgg gaggcaccgg
    15601 gtcgtcgaac gggcgctcga ggcgcaccac gttcttcatc gggggggtct tcatccgcgc
    15661 ttcgaagcgc tccttcgcgc gcgggctgac gtcgctcgag tcctgcgcgt acaccacgcc
    15721 ggtcggtccg gccgcgcgcg ccagaagctc ggtgctgtag cccccgccgg cgcccatgtc
    15781 gaggaccttc atcccgggac gcacgcgcgt gaacagcagc atcgtcgccg ggtcgcggcg
    15841 gcggtcggtc tggcggtcgg cgtcgctgcg gtccggcgcg gcgacgatcg cggcgtaatt
    15901 cgctgccccg gcggtgcgat gacccgcgca gcccgcgagc gcgaccgaga gcaggccgag
    15961 aagaacgatg ttcgtcttgg gcatggcgag ccattctacc caagctttcg gctaaactgc
    16021 ccgcctactc cggagggagg agagatcgat ggagcgacgg gccttcctgc tcggcgcgct
    16081 ggcggctgcc gcgtcgccgt gcgtgatcgc gcaggagact ccgccgggtg cagccgatgc
    16141 ggccttgcgc gagaagctcg ccacctgcgc gcgcatcttc gcgatgcagg ggctcctggg
    16201 gctgttcggt cacgtcagcg ctttcgatcc gaaaggccgc cgcgtgctga tcacacccgg
    16261 cgcgggcgtc gacaaggcga ggctgaaagg cccggacatg gtcgtcatcg acctctcggg
    16321 caaagtgctc gagggccgcg gccgaccgcc catcgagtgg ccgatccaca ccgcgctgca
    16381 cgccgcgcgc gccgacgcgc tggcggtcgc gcatctgcat ccgccgcagg cgaccttgtt
    16441 cggcatcgcc cggcgcgaat tcgcgcccgt gacggtccag ggctgcctgc tgatcgacgg
    16501 cgtgcctctc taccgggacg ccgagctggt gaccacgatt gaacgtggtc agcgtctcgc
    16561 cgcgacgatc ggagccaagc cggccctgtt catgcgcggc cacggcgtgg tcgtggccgg
    16621 caagagtctc gagcgcgttc tctacgccgc gctcatcctc gaagacgagg cgcgcaaggc
    16681 ggcgcaggcc gccgcgctcg gcgaggtcgg caccatcggc gaggaggaat gccgcgcgtt
    16741 ctcgaccgac gcggaatggg acgctcgctc gcgccgcgcg tggagctact acgcggcgat
    16801 ggaatcgaga tgggaccgcc agcccgcttc gggcatggtc ccgttctcct gatccgagct
    16861 cattcgaacg tcgtttgcct cagccggtga acgcacgcgt cgacgaacgc ccggacgttc
    16921 gccgacagga tgcgcgaatg cgggtagacg acgtgcaccg gcagcgggtc ggcctcgaac
    16981 ttctcgagga cgtacctcag ccggcgcgcg cgcttggccg gcgcggtcat gtacgagagg
    17041 aaagagccga gcccgaggcc cgcgatgcac gcgtccagcg cgccgtcgac ctggttgcag
    17101 ctcatcgcgc tggtgatcgg caccgacatc attcgcgaat ccacgcggaa cggccattcg
    17161 ctgcgcggcg caaggccggt gaagcgcacg caacggtgcg cgcgcacgtc gtcggggcgg
    17221 cgcggcgtgc cgtgcgcgcg caggtagtgc gggctggcgc agacgacgcg gcgcacctgc
    17281 ccgacgggaa tcgcgaccag cgacgagtcc tgcaacggcc cgatacgaag cgcgacgtcg
    17341 accccctcct cgatcaggtt gacgacgcga tcgacgaaca gcaggtcgac cgacagggcc
    17401 ggccaccgcc gcgcgaagtc gctcgcgagc ggcgcgacgt agcggcggcc gaacatcacc
    17461 gacgccgtca gcgtcagctt gccgtgcggc gccagtcgac gcgacgcgag cgccgcctcg
    17521 gcgtcgcgca gctgccccag gatgatgcgg cagtgctcgt gatactgccg gccttcgtcg
    17581 gtgaggcgaa ggcggcgcgt cgtgcgctgg agcagcgtcg tcccgagctc gcgctccagc
    17641 gccgcgaggg tgcgcaccac cgacggaagc gacgtgccca ggtcggcggc cgcggcagtg
    17701 aggctgccct tgtcgacgat ccgcgtgaat gcggtcatgg cgcgcagctt gtccatggcc
    17761 gggagattat tccacttttt gcaatattga actgagacct gccccattta ttcagcacgt
    17821 gcgttaccgg acactgtgcg ccggttccaa ccgggaggcc gcacatgaag cgacagtggc
    17881 ttgcagcatt tttccttctg ctctgcgccg cgggccatgc ccacgacgcg gacgaccggc
    17941 cgcaggccgg gcgcgcccag ctggggaagg tcgagtttgc caattcctgc gccgccgccg
    18001 tcgccgggga tttcaacgtc tcggtcgcgc tgctgcactc gttctgggtg aaggacgcga
    18061 tcgagggctt caacgtcgtc ctgaagcagg acccatcgtg cgcgatcgcc tactggggca
    18121 tcgcgatgtc gctgcagcag aaccccctca ccggccagga gccgagcgcg gacgccacgc
    18181 ggctcgcgct cgccgggctc gacaaggcca gggccatcgg cgccaagacg cagcgcgagc
    18241 gcgactacct cgcggccatc gacctgatct accgcgacgc cgacaagacg ccgttccgcg
    18301 cgcgccggct cgcgtacgag aaagcgatgg aggcgctcgc gcagcgctac cccgcagaca
    18361 ccgaggcgcg catcttctac gcgctgtcgc tcgacatgac cgcggatctc tccgacaaga
    18421 cctacgccaa tcagctcaag gcggccgcga tcctcgagcc gctgtcgcgc gagctgcccg
    18481 agcatcccgg gatcgcgcac tacctgatcc acagctacga ctacccggcg atcgcgtcgc
    18541 gcggcgtcga ggcggcgcgc cgctatgcgg aggtcgcgcc cggacatccg cacgccctgc
    18601 acatgccttc gcacatcttc acgcggctcg ggacctggca ggcgtccgtg gacaccaacc
    18661 ggcgctccgc cgaagcggcg aagggcgaag gcaacggcgg cgagcaggcg cacgcgatgg
    18721 actacatggt gcacgcctac ctgcagctcg gccaggacgg gcaggcgaag cgggtcgtgg
    18781 acgagagccg aacgatcacc acgctgaacc cggcgagctt cgtcggctac tatggcgtcg
    18841 cggcgatgcc cgcccggtac gcgctggagc gccgcgcgtg gaaggacgcc gtggcgctcg
    18901 agccgcgcga gacccgctgg ccgttcacga ctgcaatgac gcatttcgcg cgcggcctcg
    18961 gcttcgcgcg cctcggcgac acggcgtcgg cgcagaagga aatcgatgtg ctcgcgtccg
    19021 agcgcgacgc gctcaggagc cagagcaacg cttactgggc gaaccaggtc gaggtgcagc
    19081 ggctcgccgt cgcggcctgg actgcgctcg cgcagggcgc gcgcgacgac gcgctgaaat
    19141 cgatgcaggc ctcggccgat ctcgaggact cgatggagaa gcacatcgtg acgccctcgc
    19201 ccgtggtgcc cgcgcgggag ctgctcggcg acatgctgct cgagctgaag cggcccgcgg
    19261 aagcgctcgc ggcctacgag gcgtcggccg tccgcgagcc gaaccggctg cggggcatct
    19321 acggagcggc gcaggcggcc gcgctcggcg gcgaccgcga caaagcgcgg agctactacg
    19381 cccggctcgc ggcgctcgcg gcgggcgcga accccgggcg cccggagctg caacaggcga
    19441 aggcctacct ggcgaagcgc tgaggcggtc gcttagcggg tctcgctcat gcactgatcg
    19501 acggcgcccg gcgatttccc cgcggcggcg accgcgcccg cgcgcaggca gtcggcgagg
    19561 ttgtcggtgt agccgcgggc gtccgaagcg gggaccgtcg cgggcgcggt catcatgaag
    19621 gtcctgcggc agaacgccca gttgccgcgc accaggtccg cgcaccgggc cgcgtccttc
    19681 ttgatgtcga cgccgaggca gcgcatgaag cggccgtcct tatcgcaata ctgctgcacc
    19741 agcgccttct gcgtgccgtc ggcgagcgac gcgggatcga tgcctcccgc gccctgggcg
    19801 agcgccgtat cgacgagcac ggcctgcacg agcaatgcaa cgaccatcct gaccatggtt
    19861 ccctcctccg gacaaatgcg cttccgcgct tcgccgcggt cggctccttc ggacgggagc
    19921 cttgcgcggc gcttacaaag gatatgcaaa cggaatggcg ccggggcgcc ggaaacggcc
    19981 cgagagccaa gtaagtcacg ccgccttccg tttcgcgctc gcctcgggat acgggtagag
    20041 gatgttgagc gccacgctct gccccgcgct ctgcacgatg cgcacgtgct cgcgccgcag
    20101 ctcgcgcgcg tgccggcagc cgcacgagtg cgcgatcatg tcgatctccc ggttcatgcc
    20161 ctgcgcgtag ttcgcgacgc gctccgcctt gtcttcgacc accagcccgc gctgcagccg
    20221 cgggttgtgc gtggtgacgc cggtcgggca ggtgttctgg tggcagcgca gcgcctggat
    20281 gcagccgagc gagaacatga agccgcgcgc ggtgttcacg aaatcggcgc cgacgcacag
    20341 cgcccaggcg gcgcgcgccg ggttgaccag cttgcccgcg gcgatcacgc gaatgcgctc
    20401 gcgcagcccc gattcgatca gcgcgtcgac gacgcgcggc agcgcttccg cgatcggcag
    20461 cgccatgtga tccatcagcg cctggggcgc cgcgcccgat ccgccttcgc cgccgtcgat
    20521 cgcgaggaaa tcgggcgcga cctcgagccc gcgccggtgc actgcgtcgg cgagctcgtt
    20581 gatgaactgc cagccgccga tcgcggtctt cacgccgacg ggcttgccgg tcagatcgcg
    20641 gatgtacgcg atcttgtcga gcagctggtc gacgttggcg acgtcgcggt ggcgattcgg
    20701 gctgatcgag tcctggtgcg gcgggatgcc gcggatgccg gcgatctgct cggtgacctt
    20761 cgccgcgggc agcacgccgc ccttgcccgg cttcgcgccc tgcgacagct tgatctcgaa
    20821 cgccttgacc tccggatggc gcgcgagctc gcgcgcgcgc tcggacgaga atccgccgtt
    20881 ggccgagcgc acgccgtagt tggccgtgcc gatctgcatg atcacgtcgc agccgccctc
    20941 gagatggaac ggcgacaggc cgccctcgcc cgtgtccatc cagcagcccg ccatcttcgc
    21001 gccgcgcgag agcgcgcgca ccgcgggctc cgagatcgcg ccgaagctca tgccgctcac
    21061 gttcacgacc gatttcgcca cgaacggccg gtcgcaccag ccctcgccga tcgcgagcgg
    21121 cggcgtcggc aggcgatcct cctcgagcac cgggtacggg tgattcacga acaggatcga
    21181 tcccggctcg ttggtgttgt tcgtggagcc gaagccgatc acgccgccct cggccttcgc
    21241 caggcgatac acccagccgc gcgtcgcgcg gttgaagggc atctcctcgc ggtcgttcat
    21301 gaagaagtac tggcggaagt actcgccctg cttctcgagc aggtagcgca gccgcccgac
    21361 caccgggtag ttgcgcagca ccgagtgctt cttctgcgtg acgtcctgga cgaaccagaa
    21421 gaggaggacg ccgatcagcg cgaacccgac gatcgtcccg aggccgtagg aaacgacgct
    21481 caacattccg gacatggaat ccctccctgg gagtggaggg atgatagcgc taaggcagga
    21541 ttctgagaat cacggactcg ggatcatcgg ccgttccgtt ctcgatctcc gcaggcgtcg
    21601 cgggacgcac gtccgcgacc cgcgcgacga acttgagcgc catgccggcg agcggatggt
    21661 tgccgtccag caccacctgg ccctcggcga tgtcggtcac ggtgtagagg cccttgccgt
    21721 cgtcaccctc gacgcgcatg ccgacctcga ggccctcggg aaaatcgctc cgcggcgcca
    21781 tgcgcagcag gttctcgtcg tagtcgccga atgcgtcctc gggctccagg cgcgtctcga
    21841 cgcgctcgcc ggcggacttg ccctcgagca atgcctcgac cgcgggaaag acgttgccgt
    21901 agccgccgtg caggtactgc acgggctcgt cggacttctg gatcaggttg ccccagatgt
    21961 cggacagctc gacgtcgagc gagacgacag cgttcttggt gactttcatg gatccgcccg
    22021 ccggcgggaa aaccgcggcg ctagcgcagc gacttgacca ggctcagcac ctcggccgcg
    22081 tggcccttgg gctgcacctc gtacagcgcg tggcgcacgg tgccgcgctt gtcgatgatg
    22141 aaggtcgagc gcacgatgca gggcttcttc gtgccgttgg tctccttctc gaccagcacg
    22201 ccgtaggcgc ggcaggcttc gccctcggga tcggcgagca gccggatcga cacgccgtgc
    22261 tcgtcgcgga acgcctggtg gcgcaggcag tcgtcggggc tgacgccgat cacgatgcag
    22321 tcgtagcgcg cgaaatcctg ctcgtggtcg gagaagtcgg tggcctgcat ggtgcacccg
    22381 ggcgtgccgt ccttcgggta gaagtagagg acgacgtgct tcttgccgcg gaagtccgcg
    22441 aggtcgcacg actccatgtc ggcgtcgggc aggctgaaga ccggcgcggg ctgacctgcg
    22501 agcaacatat cccctaagcc tagacgaccc atcttccacg cacgcggagc gctctcgccc
    22561 cgtcccctgg ttcgcttcgc ggcagcttaa ctcaaaccgc gcgccccgga tcaaaagcaa
    22621 cgacagacta taatcgccgc ggtgggaatg caactgctcg gcggcctgtc ggcaaacaat
    22681 tttctgtcgc gattctggca gaagaaaccg atgctgatac gctccgcgat tcccggcttc
    22741 tcgggcatca tacaaccacc tgaattgttt cggctcgccg gccgggacga cgtggaatcg
    22801 agaatcgtcg ctcgacgcgc agggcgctgg acgctcgcac aaggaccgtt ctcgcgtgca
    22861 gacatcgagc gccagccgcg cacgaactgg acgctgctgg tgcaaggggt gaattgcgtc
    22921 cacccgcccg cggacgatct gctgcggcgc ttcgatttca ttccttacgc gcgactcgac
    22981 gatgtcatgg tcagctacgc cgctcccggc ggaggcgtgg gcccccacgt cgactcctac
    23041 gacgtgttcc tgctccaggc ctcgggacgg cgacgctggc gtttgagtgg caaagtcgca
    23101 ccggagtttc gcgaggacgc gccgctgcgc gtgctgaagc gcttccggcc cgagcgcgag
    23161 tgggtgctcg agccgggcga catgctctac cttccgcccg gctgggcgca cgagggcacc
    23221 gcggtcggcg cctgcacgac ctgctcgatc ggcttccgcg cgcccgcgcg cgccgagctc
    23281 gcgggcgagt tcctggcctg gctgggcgca cacgccgatc ttcccggcct ctaccgggac
    23341 ccgggcctgc ggccggcggc caacgcggcc gagatcccgc gcgagctggt gcgcgccgcc
    23401 gcccgggcgg tgcggtcgct gcgctggacc gagcgggatc tcgccggttt cgtcgggagt
    23461 tacctgacgg cgcccaagcc caggatcgtc ttcgatccgc cgccgcgcgc gctgtcgccg
    23521 gcggtgttcg cgcggcgatg ccgcgagaaa gggctcgcgc tcgacgcgcg ctcgcagctg
    23581 ctgttcctcg gccgcgactt tttcgtgaac ggcgagccgt tcgcgccgcg tcccgcgctc
    23641 gccccgccgc tgcggcggct cgcgcacgag cggcgcctcg gcccggggcg cgcgccggcc
    23701 gcgctttgcg ccctcgccta ccgctggtat ctttccggct gggtgcggcc cggcgacgga
    23761 catggatgac gcggttcgag aggcggcgcc gcagcgcagg ctgctggcga cccgcaagga
    23821 gtacctggag gcgatcgagc agctgctcgg cctcgtcgcg cgcgagctgc gcattttcga
    23881 tcccgacctc gccgagctcg gcatgaacgc cgagccccgg atcgcgcgcc tgcgcgcctt
    23941 cctcgcggcg agccccacgc accggttgta cgtcgcgatc cgcgacaccg acttcgtaac
    24001 ccggcgctgc gcgcgcgtgc tcgcgctgct cgggtcgtat ccgcggcaga tgttcatcca
    24061 gcagacggtc ggcgaggcgc tcaaggcgca ggactgcttc gtgatcgccg acgacaggca
    24121 tctcgtgcgg cgctcggtcg cggcccagcc gcgcggcgtg ctcgtcctcg acgacccgaa
    24181 ggattgcggc tccatacgcg atcgcttcga ggagatctgg gaatcgtcgg agccgaccgt
    24241 gtcgcccaac gtcacgggac tgtgacgcgc cggatttgac gcgggtcatg ccgcggcgca
    24301 ataaaacgcc ttagaatttg aactcggggc gtcggtattt gtcctagcct tcgccctcca
    24361 ttacccctat cagacaggag aacaacaatg aagcgcacgc ttctcatggc cgccggtttg
    24421 gcggccgccc tggcgctcgc cgcttgccag aagaacgaat cgtccacgcc caagaccggc
    24481 tcgacgccgc ccgcgtccac ggcgccgtcg acgccgccgg catcgagcgc gccctcgggt
    24541 gccccgtcct cggccgcgcc gtcctcgggt gcgccgtcgt cgagctccgc gcccgcgacg
    24601 ccgtccagcc cgtccgacgc ctcgaagaag taagcgtcgc ccgctcgggc gcaaaaaaag
    24661 ccggcctttc gggccggctt tttcatttcc gcggaggcgc tacacgtcgc gccagccgag
    24721 gccctcctcc tgcgtcgcca cggcgatcca cccgggagcg cagccgagcg cggacgagag
    24781 cgcggcgcgc gcaagctccg gcgtattgtt cttgcgcgac aggtgcgcgg ccaccacgtg
    24841 cttcagccgg ctgcggtcga gcgctgccag gatcgccgcc gacaccgcgt tgtcgagatg
    24901 gccccagcgg cccgcgatgc gcgccttcag cgacggcgga tattcgccgc ccatcagcag
    24961 gtcgacgtcg tgattgcact cgagcacgag cgcgtcgcac cccgacaggc tcgcctcgat
    25021 gtgagccgtg gcgcagccgg tgtcggtgag cacgccgagc cggtgcgcgc cgtcgccgaa
    25081 gacgtattgc acgggctcgc gcgcgtcgtg cggcacggtg agcggccgca cctcgagatc
    25141 ggcgacggcg aaggcggaat cgggatcgac cagccgcacc tcgacgtcgc ggcccgcgtc
    25201 gggctcgctc cagcgcatcg ccgagaacgt gccccaggtc atccacacgg gcagcccgaa
    25261 gcgccgcgcc agcgggaaca cgccgtcggc gtgatcggaa tgctcgtggg tgacgacgat
    25321 gccggcgagg cgcgagggct cgaggccgag acgctcgagc cgcgcgacgc agtcgcgcgg
    25381 gccgaagccg cagtcgagca tcaggcaggt cgcgcccgct tcgacgatca gggcgttccc
    25441 ctcgctgccg ctgccgagta gagcgaagcg caagcgccct cttctacttc agctggtcgt
    25501 gcagcagggc gaggatcttg cgcgccgtgt ccgagggctc cgtggcgccg tccttgttga
    25561 ggacctggac ctcgctgatc gccttcaggt ccttgacctg gacgcggtac tgctcggtgg
    25621 agccgatgcc cttgttcttg ccgaagatca gccgggagaa gaatccctgc ttgtcggtgt
    25681 cgagcgcgtc cttctcggga ttcacgtagc gcacgaagta gtagccgtgg gctcggtcgc
    25741 ggtcctcgac ggtgaagccg acgcggtcga gcgcaagccc gacgcgccgc caggcgcggt
    25801 cgaagggctc cgagagctcg agcgagccgg ttccgtcctg catcgtcacg atgcgcgcac
    25861 gctcctgctt cggaccctcg gcgacctgct gcttggcgcg cgcctcgtcc acaccgaaac
    25921 gcaccatcag gcgccgcagg aactcggcct cgagctcggg gtccgagggc cgcggctgcc
    25981 acacggtcga gtcgcgctgc tggttggtgt agacctcgac cacgccgcgg tggctgatgt
    26041 agacctcggt ggtgccgggc tgcgccccgc gctcgaggcg cgtgcggaac ttgtcgcgct
    26101 cggaggtcga gtacatgccg tcgatgacct tgcccaggat gttgcggacg atgtcgtcgg
    26161 ggatcttggc ccggttctcg gcccagtcgg tctccatgac gccgatctcg gggttctcgc
    26221 tcttgatgag aaagccggcc tcctgccaga actccttcac caccggccag acgcgctcgg
    26281 gcggcatttg caccacgagc cagcgctgcg cgccggcgcg ctcgacgtgc gcgtccttga
    26341 cctcgggcag gacatcgttc gggctggcgg tgcgcgccgc gccggcacgc tcgctgctgt
    26401 acgccgagaa cgtcgccgtg ccggtcgggc tgacgtcggg aacctggtag cggtcgtcgc
    26461 gcgtcggcga cgtgaggtcg ggcggcacgt cgagcgtcgt cgtccgctgc gcctcggcgg
    26521 acttgtagtc gattttcttg ccttcccaga agccggtggt gcagccggcc agcccgagcg
    26581 ttaccgtcgc cgcgagcagc gcgcggcgtg ctgcgcgcgt catgccgtcc tgccctccac
    26641 cacgcgcagg cccgccgggg cgaggtcggc ctgccgcatc gcctcgcgca ccacgtcgtg
    26701 gaactgcggc gagagcggcg tgagcggcag ccggatgccg ggaccgatca ggcccatctg
    26761 cgcggccgcc cacttcacgg gaatggggtt cgcctcgacg aagagcttct ggtgcagccc
    26821 gaggagcttg aagttcagct cgcgcgcgcg cttcacgtcc tcctggaggc ccgccgcgca
    26881 catctcgtgc atcaggcgcg gcgcgacgtt cgccgtgacc gagatgatgc cgctgccgcc
    26941 gaggaggatc agcgcgaggc cggtcgcgtc ctcgccgctc cagaccatga agcccttcgg
    27001 cgcgcgccgc agcaggtcgc tgccgcgctc gatgttggcc gtcgcgtcct tgatgccgac
    27061 gatgttcggc acctgcgcga gccgcagcgt cgtgtcgttc gcgagatcgg cgaccgtgcg
    27121 gcccgggacg ttgtagacga tctgcgggat gtcgacgcgc tcggcggccg cgcggaagtg
    27181 gcggtacatc ccttcctgcg tcggcttgtt gtagtacggc acgaccgaga ggcacgcgtt
    27241 cgcgccggcc ttcttcgcgg attccgtgag ctcgatcgcc tcggcggtcg agttgccgcc
    27301 ggtgccggcg atgatcggga tgcggccgcg cgagtgctcg accgcgatct tgatcagctc
    27361 cttgtgctcg tcgaagtcga ccgtgggcga ctcgccggtg gtgccgacca cgacgatgcc
    27421 gtcggtgccc tcggcgatgt gccagtcgat cagcgcgcgg aagcgcggca ggtcgaggcg
    27481 cccgtcctcg tgcatgggcg tgacgatggc gacgatgctg ccgcggggaa gagagggctt
    27541 cgtcatcagg cggatattct atcgcggctc gagctcgggg ggccattcgg actcgcgcac
    27601 ggcgcaaagg cgctgcacca cggcaggctt gggccgcgcc acgacgccct gctcgaaggc
    27661 caccggccga agcgcccgcg tgcgctccag caattcgttg ggctgcaaca ggaaatccgg
    27721 attgcccgga cggccgtagc gctcgttgcc gagcgcgaag gtctcgtaga tcagcacgcc
    27781 gccgggggcg agcgcgtcga ccagccgcgg gaacagcggc cggtgcaggt agttcgtgac
    27841 gacgacgccg tcgaagcgct cgtcgccgag cggccacggt ccgccctcga gatcggcgac
    27901 gcgcagctcg acgccgggaa cgccggcgag cgccgccgga tcgcgatcga cgccgagcac
    27961 gcgcaggccg aggcccgcga gaaagcgagc gtgccggccg cccccgcagg cgagatcgag
    28021 cacgcgcccg cccggccgca cgaggtgcgc ccagcggcgc acccagtcgg agggagattc
    28081 gagatcgtga gacatggcgg ctccagttgt agcacactgc gagcggtacg gaggaaccgc
    28141 catggccccc agctcgaaac cgatcacctt ccccggcgcg tcgggcgcaa tgctcgccgc
    28201 gcgcctcgac ggcccgctcg cgccgccgct cgcgtacgtg ctgttcgcgc actgcttcac
    28261 ctgctcgaag gacacgaagg gcgcggtgta catcagcgag gcgctcgcgg aggccgggct
    28321 ggccgtgctg cgcttcgact tcaccgggct cggcgggagc gagggcgact tcgccaacac
    28381 caatttcagc tcgaacgtcg ccgatctcgt cgccgccgcc gactggctga ggcgcgagca
    28441 caaggcgccg gcgatcctgg tcggccacag cctcggcggc gcggccatga tcgccgccgc
    28501 ggcgaagatc ccggaggcgc gcgcgctggt gacgatcggc tcgcccgcgg agccggcgca
    28561 cctcggccac ctgctgcgcg actcgcgcga gaccatcgag cgcacgggcg aggcgacgat
    28621 cgacatcggc gggcggccct tccatgtgaa aaagcagctg ctcgaggaca tcgagtcgca
    28681 gaagatcgcc gaggcgctgc cgcgcctggg gaaggcgctg ctcgtcatgc actcgccgaa
    28741 ggacacggtc gtcggcatcg acaacgcctc gaagatcttc ctcgcggcga agcacccgaa
    28801 gagcttcgtc tcgctcgacg acgccgatca cctgctcggc cgccgcgccg atgcccgcta
    28861 cgccgccggc gtgctcgcgg cctggtcgca gcgctacctc ggcgtcgagc ccgagaaagt
    28921 gcccgaggcg cccgaaggcc gcgtgctcgt gcgcgagacg cgcgagggca agttcaccaa
    28981 ccaggtcgtc gtcggccggc acgtgttccg cgccgacgag ccggtcgcgg tcggcggcaa
    29041 cgacaccggg ccctcgccct acgacctgct gtgcgcggcg ctcggcgcct gcaccgcgat
    29101 gaccatccgc tcgtacgcgg acctgaagaa gctgcccctc gagcgcgcct cggtcgagct
    29161 gcgccacgac aagatctacg cggccgactg cgccgactgc gagacgcgcg agggcaagat
    29221 cgaccgcatc gagcgcgtcg tgcgcctcga gggcgacctc aaccccgagc agcgcgcgaa
    29281 gctcctcgag atctcgaaca agtgcccggt gcaccggacg ctgcactcgg agatcagcat
    29341 cgtcaccgcg ctcggggatt gagctttcgc ttgtcatcct gagcaaagcg aaggatctgc
    29401 ttttttctaa aacctcgacc ccggctgctt caggaactcg atctcctccc gcgtcgactc
    29461 gcgccctaga atctcgttcc ggtgcggaaa gcgaccgaag cgcgagacga tatcgtgatg
    29521 ccggcgggcg aagtcgcgat gatccacgcc gaggatcgcg agctcgtcgt agagcgccac
    29581 cgagcggcgc tgaatttcga cgtcttccgc gtgctggaac ggcagataca cgaacgcgcg
    29641 ctcgatcggc cgcagcaggc ggtcgaagcg ccgctcgatc atgtcctccg ccgtcgccag
    29701 cgcaagcgcg tcggacgcga acgcgcgcgg ctcgccgcgg aacatgttcc ggggaaactg
    29761 gtcgaggacg accacgagcg cgagcgccgc gagcggcgtc ccgcgccagg atgccaaggc
    29821 gccgcccgag gccttcgtcc agagcgacga gaaccgctcc cggatttcga gatcgaagtc
    29881 gtccgacttg gtgaacca
//
LOCUS       DATDMW010000142         5697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1910914, whole genome shotgun sequence.
ACCESSION   DATDMW010000142 DATDMW010000000
VERSION     DATDMW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910914"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..618
                     /locus_tag="VI319_06325"
     CDS             88..618
                     /locus_tag="VI319_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY6863499.1"
                     /translation="MASGDGHLWRDPAEIRPAAVLVPVVRRDDLTVLFTRRTPHLQDH
                     AGQISFPGGRTEPGDPSLEATALREAQEEIGLAQERVELLGRLPEYVTVTGYRVTPVV
                     GLVSPPLDLRPDPFEVAEIFEVPLAFLLDPANHLRNTVLQEGKRRRYYAMPYREYYIW
                     GATAGMLMNLYGFLRA"
     gene            625..1545
                     /locus_tag="VI319_06330"
     CDS             625..1545
                     /locus_tag="VI319_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEY6863500.1"
                     /translation="MGLAALIAALLLEQWRPLVERRYLFSLAVRYAMFLEQQFNAGEA
                     EHGAIAWLIAVLPPVLATWAVYALLWRVSPLLALAFNVVALYFTMGFRQFSHHFTEIH
                     LALKQDDVTRARDVLSQWLGQRCDELDREALVRITIEQALVASHRHVFGVVFWFVLLP
                     GPSGAVLYRLAVFLKRRWAGAHSDTGPFGRFSARALALLEWPALRATAASFAIVGDFE
                     DAVYCWRTQAASWPDPGTGIVLAAGAGALGVKLGMPVYEQGSLVDRPPLGVGDDADTG
                     FLDSAIGLVWRALILWLLLLLLLGIANAVR"
     gene            complement(1556..1861)
                     /locus_tag="VI319_06335"
     CDS             complement(1556..1861)
                     /locus_tag="VI319_06335"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY6863501.1"
                     /translation="MKVVFSSLNLVELHHLKNLLESAGIRCRIERELLSRLAGEIPFP
                     ECAARLVVERPEDEASAEEIVRDFGRPRRPGAPWTCASCGERLEGQFTACWRCGASR"
     gene            complement(1858..2745)
                     /gene="rsgA"
                     /locus_tag="VI319_06340"
     CDS             complement(1858..2745)
                     /gene="rsgA"
                     /locus_tag="VI319_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY6863502.1"
                     /translation="MSPAALVSGRVVAAYGRRFLVETGSGIRSCIVRGRTSEVVCGDR
                     VALRPAEADGGVIEAVDPRSSLFMRSAPHRAKPIAANVSQVAVVVATDPSFSDEMIAR
                     VLAAAEHQRLKSCIVLNKVDLPAVTARERLEPFAAAGCRIVETSALGDIANLRAALRG
                     ETTLLLGQSGMGKSTLVNALVPAAQAKTREISKFLASGRHTTTSVMLYRLDAESAIVD
                     APGMQEFGLAHLAPGDIERGFVEFAPFLGHCRFPDCRHGSEPGCALAAAAQDARIHPR
                     RLELFRRIVAAERASGSAR"
     gene            complement(2742..3074)
                     /locus_tag="VI319_06345"
     CDS             complement(2742..3074)
                     /locus_tag="VI319_06345"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY6863503.1"
                     /translation="MMTRLADEHSRALPAGTAPLSRAEIDALLAEVPGWSYDGRVISK
                     SYSFEDYHRTIAFVNALAWIAHREDHHPDLAVGYNRCRVEFSTHSIGGISRNDFICAA
                     RIERLLSP"
     gene            complement(3074..4321)
                     /locus_tag="VI319_06350"
     CDS             complement(3074..4321)
                     /locus_tag="VI319_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY6863504.1"
                     /translation="MHWFTALFLLALAVSTATRLWLSLRQLRHVTRERRAVPAGFEDS
                     IPLSAHQKAADYTVAKTRLGLLETAVGALVVLAFTVGGGLQRLIEAWSGVLDPGGYAH
                     GVALVVSVVVVSGLVDLPFSAVRAFVIEQRFGFNRMTPALFIADLAKQALLSALLGIP
                     LLFCVLWLMARAGEAWWLYAWLTWVAFSLVVHFLYPTVFARWFNKFSPLTDAALKARI
                     ESLLAKCGFRAKGLFVMDGSKRSSHGNAYFTGFGAAKRIVLFDTLISRLQPPEIEAVL
                     AHELGHYSLHHIWKRMALVFAGSLATLWLLGWLAGQDWFYTGLGVQTPGTAVALLLFF
                     MAVPAFTFPLHPLASLYSRKHEYEADAYASRHADAAELARALVKLYEDNAATLTPDPL
                     HSAFYDSHPPAVHRIARLQTLGA"
     gene            4413..4964
                     /gene="orn"
                     /locus_tag="VI319_06355"
     CDS             4413..4964
                     /gene="orn"
                     /locus_tag="VI319_06355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY6863505.1"
                     /translation="MAKDQNNLVWLDMEMTGLNPDSDRIIEVAVVITDSRLDTVATAP
                     VLVVHQSDEALAAMDDWNKSTHGRTGLIDKVRASVLTEAGAEDLLLAFVSEHVPGKTS
                     PLCGNSICQDRRFLARYMPRLEAYLHYRNLDVSTLKELARRWRPQISKGLAKQGKHEA
                     LADIHESIDELKYYRDNFLRLAP"
     gene            complement(4966..>5697)
                     /locus_tag="VI319_06360"
     CDS             complement(4966..>5697)
                     /locus_tag="VI319_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6863506.1"
                     /translation="PEAISAAVCSFADMAGAVSTVIQTLQAGIPIARSEALCGTTMKA
                     INAHSKTSWREQPTLFLEFHGSQAGVEEQARLVQEIARENGGMDFEWTTKPEDRNRLW
                     SARHQAYFACLQLRPGSRAVSTDVCVPISRLTECIVETSKDIARASMPIPLFGHVGDG
                     NFHCEILIRPDDEAELAEARAFNERVVNRALAMEGTCTGEHGIGFGKMKSLRKEMGDA
                     VDLMVALKRALDPENLMNPGKVVDF"
BASE COUNT          881 a         1974 c         2014 g          828 t
ORIGIN      
        1 gaaccagaac cacgaggcgt gtcggaggag gcgggctcgg agccctggct gcgcgcgcgg
       61 ttcgcgcgcg acgcgcgccc ggagccgatc gcgtccggcg acgggcacct gtggcgggac
      121 ccagccgaga tccgccccgc cgcggtcctg gtcccggtcg tgcgccgcga cgacctgacc
      181 gtcctcttca cgcgccgcac gccgcatctg caggaccacg cgggccagat cagcttcccc
      241 ggcgggcgca ccgagcccgg agacccgtcg ctcgaggcga cggcattgcg cgaggcgcag
      301 gaggagatcg ggctcgcgca ggagcgcgtc gagctcctcg ggcgtctgcc cgagtacgtc
      361 accgtgaccg gctaccgcgt gacgccggtg gtcgggctgg tgtcgccgcc cctggacctg
      421 cgtcccgacc cgttcgaggt cgccgagatc ttcgaggtgc cgctcgcgtt cctgctcgac
      481 ccggcgaacc atctgcgcaa caccgtgctc caggagggca agcgccggcg ctattacgcg
      541 atgccgtacc gcgagtacta catctggggc gcgaccgccg ggatgctcat gaacctgtac
      601 ggcttcctgc gggcctagag cggcatgggc ctcgccgcgc tcatcgccgc gctgctgctc
      661 gagcaatggc gtcccctggt cgagcggcga tacctgttct cgctcgccgt gcgctacgcg
      721 atgttcctcg agcagcagtt caacgccggc gaggccgagc acggcgcgat cgcctggctg
      781 atcgccgtgc tgccgccggt gctcgcgacc tgggccgtgt acgcgctcct gtggcgggtg
      841 agcccgctgc tcgcgctcgc cttcaacgtc gtggcgctct acttcacgat gggcttccgc
      901 cagttcagcc accacttcac cgagattcat ctcgcgctca agcaggacga cgtgacgcgc
      961 gcgcgcgatg tgctctcgca gtggctcggg cagcgctgcg acgagctcga ccgcgaggcg
     1021 ctggtgcgca tcacgatcga gcaggcgctg gtcgcctcgc accgccacgt gttcggagtg
     1081 gtgttctggt tcgtgctgct gccggggccg agcggcgcgg tgctgtaccg gctcgcggtt
     1141 ttcctcaagc ggcgctgggc cggcgcgcac agcgacaccg gaccgttcgg ccggttctcg
     1201 gcgcgcgcgc tggcgctgct cgaatggccg gcgctgcgcg cgacggcggc cagcttcgcg
     1261 atcgtgggtg acttcgagga cgcggtctac tgctggcgca ctcaggcggc gagctggccc
     1321 gatcccggga cgggcatcgt gctggccgcg ggcgcgggcg cgctgggggt gaagctcggc
     1381 atgccggtct acgagcaagg cagcctcgtc gaccgcccgc cgctcggcgt cggcgacgac
     1441 gccgacacgg gtttcctcga cagcgcgatc gggctcgtct ggcgcgcgct gatcctgtgg
     1501 ctgctgctgc tgctcctcct cggcattgcg aacgcggtgc gctgaagcgc gcgcttcagc
     1561 gagacgcgcc gcagcgccag cacgcggtga actgaccctc cagccgctcg ccgcagcttg
     1621 cgcaggtcca cggcgcgccg ggccgccggg gacggccgaa atcgcgcacg atctcctccg
     1681 cggacgcctc gtcctcgggc cgctcgacaa cgaggcgggc ggcgcactcg gggaacggga
     1741 tctcgcccgc gaggcgcgag agcagctccc gctcgatccg gcagcggatg cccgccgact
     1801 cgagcaggtt cttgagatgg tggagctcga ccaggttgag cgaggagaag acgaccttca
     1861 ccgggccgac cccgacgcgc gctcggcggc gacgatgcgc cgaaagagct cgagccggcg
     1921 cgggtggatg cgcgcgtcct gcgccgcggc ggcgagcgcg cacccgggct cgctgccgtg
     1981 gcggcagtcc gggaagcggc aatgcccgag gaacggcgcg aactccacga atccgcgctc
     2041 gatgtcgccg ggcgcgagat gcgcgaggcc gaactcctgc atcccgggcg cgtccacgat
     2101 cgcgctttcc gcgtccagcc ggtacagcat cacgctggtc gtcgtgtgcc ggccggacgc
     2161 gaggaacttc gagatttcac gcgtcttcgc ctgtgccgcg ggcacgagcg cgttcacgag
     2221 cgtcgatttt cccatcccgg attgtccgag aagcagcgtg gtctcgccgc gcagcgcagc
     2281 gcgcaggttg gcgatgtcgc ccagcgcgct ggtctcgacg atgcggcagc ccgccgcagc
     2341 gaaaggctcg agacgctcgc gcgccgttac ggccgggagg tcgaccttgt tcagcacgat
     2401 gcacgacttc aggcgctggt gctcggccgc ggcgagcacg cgcgcgatca tctcgtcgga
     2461 gaagctcggg tcggtcgcga cgacgacggc gacctggctg acattggcgg cgatgggctt
     2521 ggcgcggtgc ggcgcggagc gcatgaacag cgacgagcgc ggatcgaccg cctcgatcac
     2581 gccgccgtcc gcttccgctg ggcgcaaggc gacgcggtcg ccgcacacca cctcgctggt
     2641 gcggccgcgc acgatgcagg agcggatgcc cgatccggtc tcgacgagaa agcgccggcc
     2701 gtacgcggcg accacgcggc ccgagaccag ggccgcgggg ctcacgggct cagcagccgc
     2761 tcgatcctcg ccgcgcaaat gaaatcgttc cgcgatatcc cgccgatgga gtgtgtgctg
     2821 aactccacgc ggcagcgatt gtagccgacc gcgaggtcgg gatgatggtc ctcgcggtgc
     2881 gcgatccagg cgagcgcgtt cacgaacgcg atcgtacgat ggtagtcctc gaacgagtac
     2941 gacttggaga tcacgcggcc gtcgtagctc cagcccggga cctcggcaag gagcgcgtcg
     3001 atctcggccc gggagagcgg cgcggtgccc gcgggcagcg cgcgggaatg ctcgtcggcg
     3061 aggcgtgtca tcatcaggct ccgagcgtct gcagccgcgc gatgcggtgc accgcgggcg
     3121 gatgcgaatc gtagaacgcc gagtgcagcg ggtcgggcgt cagcgtggcg gcgttgtcct
     3181 cgtagagctt caccagcgcg cgcgcgagct ccgccgcgtc ggcgtgccgc gaggcgtagg
     3241 cgtccgcctc gtactcgtgc ttgcgcgagt acaggctggc gagaggatgc agcgggaagg
     3301 tgaacgccgg aaccgccatg aagaagagca gcagcgcgac cgcggtcccg ggcgtctgca
     3361 cgccgagccc ggtgtagaac cagtcctggc cggcgagcca gccgagcagc cacagcgtcg
     3421 cgaggctgcc ggcgaagacc agcgccatcc gcttccagat gtgatgcagc gagtaatggc
     3481 cgagctcgtg cgcaagcacc gcttcgatct cgggcggctg caggcgcgag atcagcgtat
     3541 cgaacagcac gatgcgcttc gccgcgccga agccggtgaa gtaggcgttg ccgtggctgg
     3601 agcgcttcga gccgtccatg acgaacaggc ctttggcgcg gaacccgcac ttcgcgagca
     3661 gcgactcgat gcgtgccttc agcgccgcgt cggtcagggg cgagaacttg ttgaaccagc
     3721 gcgcgaacac ggtcggatac aggaagtgca ccacgaggct gaacgccacc cacgtgagcc
     3781 acgcatacag ccaccacgcc tcgccggcgc gcgccatgag ccagagcacg cagaagagca
     3841 aagggatccc gaggagcgcg ctgagcagag cctgcttcgc gaggtctgcg atgaacagcg
     3901 ccggcgtcat gcggttgaag ccgaagcgct gctcgatgac gaaggcgcgc accgcgctga
     3961 acggcagatc cacgaggccc gagacgacca cgaccgacac gacgagggcg acgccgtgcg
     4021 cgtagccccc gggatcgagc acgccggacc aggcctcgat cagcctctgc agtccgccgc
     4081 cgacggtgaa cgcgagcacc acgagcgcgc ccacggcggt ctcgagcagg ccgagccgcg
     4141 tcttcgcgac cgtgtagtcg gccgccttct gatgtgccga gagagggatg ctgtcttcga
     4201 atcccgccgg gaccgcgcgg cgctcgcgcg tcacgtgccg cagctggcgc agggacagcc
     4261 acaggcgtgt cgcggttgag accgcgagcg cgaggaggaa gagcgcggtg aaccagtgca
     4321 tgggtcggcc ggggatgcgc gcagcgacgt tgtgcgacaa ttgcgatttt atacgcgaac
     4381 gcggctcgtc acagcgcgca tcaccgcgcc acatggccaa ggatcagaac aacctcgtct
     4441 ggctcgacat ggagatgacg ggcctcaacc ccgacagcga ccgcatcatc gaggtcgccg
     4501 tcgtgatcac cgattcgcgg ctcgacacgg tcgcgaccgc gccggtgctg gtcgtgcatc
     4561 agtccgacga ggcgctcgct gccatggacg actggaacaa gagcacccac gggcgcaccg
     4621 gcctgatcga caaggtgagg gcctcggtgc tgaccgaggc gggcgccgag gacctgctgc
     4681 tcgcgttcgt gtccgagcac gtcccgggca agacctcgcc gctgtgcggc aactcgatct
     4741 gccaggaccg ccgcttcctc gcgcgctaca tgccgcggct cgaggcctac ctgcactaca
     4801 ggaacctgga cgtcagcacg ctgaaggagc tggcgcgccg ctggcgcccc cagatctcca
     4861 agggtctcgc aaagcagggc aagcacgaag cgctcgcgga catccacgag tccatcgacg
     4921 agctgaagta ctaccgcgac aacttcctga ggctcgcgcc ctagctcaga agtcgaccac
     4981 cttcccggga ttcatcaggt tctccgggtc gagcgcgcgc ttgagcgcga ccatgagatc
     5041 gaccgcgtcg cccatttcct tccgcagcga tttcatcttc ccgaatccga tgccgtgctc
     5101 gcccgtgcag gtgccttcca tcgcgagcgc gcgattgacc acgcgctcgt tgaaggcgcg
     5161 cgcctcggcg agctcggcct cgtcgtcggg acggatgagg atctcgcagt ggaagttgcc
     5221 gtcgccgacg tggccgaaga gcggaatggg catcgacgcg cgcgcgatgt ccttcgaggt
     5281 ctcgacgatg cactcggtca ggcgcgagat cgggacgcag acgtcggtcg agaccgcgcg
     5341 cgagccgggc cgcagctgca ggcacgcgaa gtacgcctgg tgccgcgccg accacagtcg
     5401 gttgcggtcc tcgggcttcg tcgtccactc gaaatccatg ccgccgttct cgcgcgcgat
     5461 ctcctggacc aggcgcgcct gctcctcgac gcccgcctgg ctgccgtgga attcgaggaa
     5521 cagcgtcggc tgctcgcgcc agctcgtctt cgagtgcgcg ttgatcgcct tcatcgtcgt
     5581 cccgcacagc gcctcgctgc gcgcgatcgg gatgcccgcc tgcagcgtct ggatcaccgt
     5641 gctcacggcg ccggccatgt cggcgaagga gcagaccgcg gccgagatcg cctcggg
//
LOCUS       DATDMW010000143         2675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2409743, whole genome shotgun sequence.
ACCESSION   DATDMW010000143 DATDMW010000000
VERSION     DATDMW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409743"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VI319_06365"
     CDS             <1..344
                     /locus_tag="VI319_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863507.1"
                     /translation="IELKNRGAAMHADYVQIYTVTEPHSETGCYDNKFVIRGTAFKAA
                     RATVPAAKAADSDSAETAPKAAPASSCDPPCSPGFACKAGACEPVCNPACEKGETCTR
                     KRICEPTPAGA"
     gene            complement(400..1155)
                     /locus_tag="VI319_06370"
     CDS             complement(400..1155)
                     /locus_tag="VI319_06370"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY6863508.1"
                     /translation="MNLTLHVWRQRAADAPGAFVRYEIASAAPEMSFLEMLDALNERL
                     AREGGDPIAFDSDCREGICGACGFVINGVAHGPQAGGTVCQLHLRSFRDGEELWLEPW
                     RARAFPIVRDLVVDRGALDRVIAAGGYVTAPTGSAPEANSVLVAKPDADAAMDAAACI
                     GCGACVAACPNASASLFTAAKVAHLAHLPQGQPERGARALGMVAQAEREGFGGCSNVG
                     ACEAVCPKGIRASTISLLNRDHLLAALAGAPRS"
     gene            complement(1152..>2675)
                     /gene="sdhA"
                     /locus_tag="VI319_06375"
     CDS             complement(1152..>2675)
                     /gene="sdhA"
                     /locus_tag="VI319_06375"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HEY6863509.1"
                     /translation="VWRLFYDTVKGGDFRSREANVYRLAQISTAIIDQCAAQGVPFAR
                     EYGGTLANRSFGGAQVSRTFYARGQTGQQLLLGAYQALSRQIHAGKVAMYPRTEMLDL
                     IVAGGHAKGSNATAIWRAHRRGAAFANPCFTQIHPTCIPVTGEHQAKLTLMSESLRND
                     GRVWVPKTRGDARAPAAIPEAERDYFLERRYPSFGNLVPRDIASRAAKDVCDAGLGVG
                     PGGYGVYLDLADALGRLGRRALVERYGNLFEMYQRITDEDPLAVPMRIYPAPHYTMGG
                     LWVDYALMSTIPGLFVLGEANFSDHGANRLGASALMQGLADGYFVAPPAVGDYLAGAK
                     LAAVDARHAAFTDAEAEAGRRLDGLLAARGRRTADDFHRELGKLMWDACGMSRAAGGL
                     RAALDRLPALREEFARDVAVPGGGRELNQALERAGRVSDFLELAELMCRDALARDESC
                     GSHFRVEHQTSEGDARRDDARFAHVAVWEHAGAGEPPRRHEEPLTVTEVAPSRRSYQ"
BASE COUNT          374 a          957 c          986 g          358 t
ORIGIN      
        1 tgatcgagct caagaaccgg ggcgcggcca tgcacgcgga ctacgtccag atctatacgg
       61 tcaccgagcc gcactcggag acgggatgct acgacaacaa gttcgtgatt cgcgggacgg
      121 cgttcaaggc cgctcgcgcg acagtgccgg ccgccaaggc ggctgactcg gattcagcgg
      181 agacggcgcc gaaggccgcg cccgccagca gctgtgaccc accctgctcg ccaggatttg
      241 cctgcaaggc gggcgcttgt gagccggttt gcaacccggc ctgcgagaag ggcgagacct
      301 gcacgcgcaa gcgcatctgc gagccgacgc ccgcgggcgc ctgattcgat ctcgtagacc
      361 gcgtgctggg ccagctccga ttcacctccg actgtgctgt caggatcgcg gcgcacccgc
      421 tagcgccgcg agcaggtggt cgcggttcaa gagcgagatc gtcgacgcgc ggatgccctt
      481 ggggcacacg gcctcgcatg cgcccacgtt gctgcagccg ccgaagccct cgcgctcggc
      541 ctgcgcgacc atgccgaggg cgcgcgcccc gcgctcgggc tgcccctgcg gcagatgcgc
      601 gaggtgggcg accttggcgg ccgtgaacag cgaggcggac gcgttcgggc acgcggcgac
      661 gcacgcgccg cagccgatgc aggccgcggc gtccatggcg gcgtcggcgt cgggcttcgc
      721 gacaagaacg gaattggcct cgggcgcgct gccggtgggc gccgtcacgt acccgccggc
      781 cgcgatgacc cggtcgagcg cgccgcggtc gacgacgagg tcacggacga tcgggaacgc
      841 gcgcgcgcgc cagggctcga gccagagctc ctcgccgtcg cggaagctgc gcaggtgcag
      901 ctggcacacc gtcccgcccg cctgcgggcc gtgcgcgacg ccgttgatga cgaagccgca
      961 ggcgccgcag atgccctcgc ggcagtcgct gtcgaacgcg atggggtcgc cgccctcacg
     1021 cgcgaggcgc tcgttcaacg cgtcgagcat ctcgaggaac gacatctcgg gcgcggcgct
     1081 cgcgatctcg tagcggacga acgcgccggg cgcgtccgcg gcgcgctgcc gccagacgtg
     1141 caatgtcagg ttcattggta gctcctccgg ctcggcgcga cctcggtgac ggtcagcggc
     1201 tcctcgtggc ggcgcggcgg ctcccccgcg ccggcgtgct cccacacggc gacgtgcgcg
     1261 aaccgcgcgt cgtcgcgccg cgcgtcgccc tcggacgtct gatgctcgac ccgaaagtgg
     1321 ctgccgcagg attcgtcgcg cgcgagggcg tcgcggcaca tgagctcggc gagctcgagg
     1381 aagtcggaga cgcgccctgc ccgctcgagc gcctggttga gctcgcggcc gccgcccggc
     1441 acggcgacat cgcgcgcgaa ctcctctcgc aacgccggca gccggtcgag cgccgcgcgc
     1501 agcccgcccg ccgcgcgcga catgccgcac gcatcccaca tgagcttgcc gagctcgcgg
     1561 tggaagtcgt cggccgtgcg gcgcccgcgg gcagccagca agccgtcgag gcgccgccca
     1621 gcctcggcct cggcgtcggt gaacgccgcg tggcgcgcgt cgacggccgc gagcttggcc
     1681 cccgcgaggt aatcgcccac ggcgggcggc gcgacgaagt atccgtcggc gagcccctgc
     1741 atgagcgccg acgcgcccag tcggttggcg ccgtggtcgg agaagttcgc ctcgcccagg
     1801 acgaacagcc cggggatcgt gctcatgagc gcgtagtcga cccataggcc gcccatcgtg
     1861 tagtgcggcg ccgggtaaat gcgcatcggc acggccagcg ggtcctcgtc ggtgatgcgc
     1921 tggtacatct cgaacaggtt gccgtagcgc tcgacgagcg cacgccggcc caggcggccc
     1981 agcgcgtcgg cgaggtcgag atagacgccg tagccgcccg gcccgacgcc gagacccgcg
     2041 tcgcacacgt ccttggccgc gcggctcgcg atgtcgcggg gcacgaggtt gccgaacgac
     2101 ggatatcggc gctcgaggaa gtagtcgcgc tcggcctcgg ggatcgcggc gggcgcgcgc
     2161 gcgtcgccgc gcgtcttcgg cacccagacg cggccgtcgt tgcgcagcga ctcgctcatg
     2221 agcgtgagct tcgcctggtg ctcgcccgtc acggggatgc aggtcgggtg gatctgcgtg
     2281 aagcacgggt tcgcgaaggc ggcgccgcgc cggtgggcgc gccagatcgc cgtggcgttc
     2341 gagcccttgg cgtgcccgcc ggccacgatc aggtcgagca tctcggtgcg cgggtacatc
     2401 gctaccttgc cggcgtggat ctgccggctg agcgcttgat acgccccaag cagcagctgt
     2461 tggccagtct gcccgcgcgc gtagaacgtg cgcgagacct gggcgccgcc gaacgagcgg
     2521 ttggcgagcg tgccgccgta ctcgcgcgcg aacggcactc cctgcgcggc gcactggtcg
     2581 atgatcgcgg tcgagatctg ggcgaggcgg tagacgttgg cctcgcgcga gcggaagtcg
     2641 ccgcccttca ccgtgtcgta aaagagccgc cagac
//
LOCUS       DATDMW010000144         5093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_540258, whole genome shotgun sequence.
ACCESSION   DATDMW010000144 DATDMW010000000
VERSION     DATDMW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540258"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VI319_06380"
     CDS             complement(<1..904)
                     /locus_tag="VI319_06380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6863510.1"
                     /translation="MRIALVRQRYNPHGGAERFIERVLGAFPGGGATFSVFARRWTGA
                     RAGIEVVRCDPFYLGRVWRDAGFERAVCRALAARRFDLVQSHERIACCDIYRAGDGVH
                     AQWLENRRRALGPVGRLGIACNPAHRYTLAAERRLFASPRLRAVICNSQMVKDDIAGR
                     FGVPLSRLHVIYNAVASDAFSPALRKHRRSVRAQFGIPEQAPLFLQVGSGFERKGVAT
                     SIDALAQLQAPSRLIVVGGDKDLRRYEARARRSSGRVTFAGPQSDPKPFYGAADAFVL
                     PTLYDPCPNAALEAMACALPIVTST"
     gene            complement(913..1776)
                     /locus_tag="VI319_06385"
     CDS             complement(913..1776)
                     /locus_tag="VI319_06385"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY6863511.1"
                     /translation="MRLARGLVAALTLLASAHAAATAIEVRDDRGVTVVLDAPARRIV
                     ALAPHLAEIAYAAGAGDRLVGVSNFSDFPPAAKRLPVVFDWGRVDYERVIQLRPDVVL
                     AWRSGTPALQVERLERLGLKVVVTEVRHFADVAALLRTVGRIAGTGASAGAAARDFER
                     RLATLRAEYAARPELSVFLEVWHTPMITINGEHVFSEAIELCGGRNVFAQARTATPVV
                     SREQLLGARPEVVVTGSAAERDAWSGLDAVPAVRTHRVYAVDANDLHRPGPRLLDGAR
                     ELCIALDRARG"
     gene            complement(1773..2861)
                     /gene="rfaQ"
                     /locus_tag="VI319_06390"
     CDS             complement(1773..2861)
                     /gene="rfaQ"
                     /locus_tag="VI319_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HEY6863512.1"
                     /translation="MPYGPADAIDPGALSRALVIKLRHHGDVLLASPVFSVLKNLAPR
                     IEIDALVYADTRDMLSLHPAIAEIHVIDRAWKRSGALAQARAELALFSRLRARRYDLV
                     VHLSEHPRGAWLARALGARWAVAPDYADKPRYWKRSFTHRYALPKGARRHMVELNLDA
                     LRRLGIRPGDDERRLVLVPGEAAERRVDALLAQAGLAAKGFVHFHPGSRWQFKCWPAD
                     RAAALLDELERRGHRVVLTAAPDPAERELVDDMLSRTRVKPLDLSGQLTLKELAALAG
                     RARLFVGVDSAPMHIAAAMQTPVVALFGPSGELEWGPWQVAHRVVASTVHPCRPCGND
                     GCGGGKVSECLTHLPLASVLGAVDALLR"
     gene            complement(2919..3857)
                     /locus_tag="VI319_06395"
     CDS             complement(2919..3857)
                     /locus_tag="VI319_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY6863513.1"
                     /translation="MRIVSLLPAATEIVAALGFADSLVGVSHECNYPPEVNGRPRVTH
                     CEVYGKGLPSAEVDRWVSGELSSNGTLYTIDEPLLRRLAPDVIITQQLCDVCAIDYRT
                     ISAIAHSLPGKPQLVSLSPSSFADVLDDIRRVAAALGAPERGDALVESLERRVEAVHA
                     KASRAQSRPRCVQLEWIDPLYCSGHWSPELVEIAGGVDPLGLKGKPSVRTAWDKLVEA
                     APDILVLACCGYEIERTLADLRILQSRRAWDSLPAVREDRVYAVNGCAYFSRPGPRLV
                     DSLEIMAEIIHPEIFPRRSAASDLVRVETPRHIASS"
     gene            complement(3862..4377)
                     /locus_tag="VI319_06400"
     CDS             complement(3862..4377)
                     /locus_tag="VI319_06400"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEY6863514.1"
                     /translation="MKHGLVVLLIVMALAGRLLPHPDNFTPVLAIALFGGAMLPGAAA
                     FAVPVTALFLSDLALGNSFGWMTAVVYGAFLAGTGIGRWLATQRTWTRTGFAAVGGSL
                     LFYLVTNFAVWALPGNHAPLYPPTLDGLAESYWMGLPFLRNGLAGDLFWTAVLFGAFD
                     LVRSRAGRAPA"
     gene            complement(4391..>5093)
                     /locus_tag="VI319_06405"
     CDS             complement(4391..>5093)
                     /locus_tag="VI319_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HEY6863515.1"
                     /translation="RAAPRPPETGAPDRVAERRGGAPVRIAVARDAALHFYYQENLDL
                     LACKGAQIAPFSVLDDDRLPGGAHGLYLGGGFPELFAEKIAANATMLDAIRVFAAAGG
                     PIYAECGGLMILCDALVDLEGRRHAMAGLVPATSVMRRDQLSLGYVEVEALRDTMLAR
                     AGDRYRAQTFHHSVLEDARFAPALKLYHGGKTTLDGCAEGNLLASYVHAHFAAQPALA
                     ERFVERCRDTMKTTH"
BASE COUNT          766 a         1804 c         1909 g          614 t
ORIGIN      
        1 ccgtgctcgt cacgatcggc agcgcgcagg ccatcgcctc cagcgccgcg ttcgggcacg
       61 gatcgtagag cgtcggcagc acgaaggcgt cggccgcgcc gtagaacggc ttcggatcgc
      121 tctgcgggcc ggcgaacgtg acccgccccg atgagcggcg ggcgcgcgcc tcgtagcgcc
      181 gcaggtcctt gtcgccgccc accacgatca ggcgcgaagg cgcctggagc tgcgccagcg
      241 cgtcgatcga cgtcgccaca cccttgcgct cgaatcccga tccgacctgc aggaacaaag
      301 gcgcctgctc ggggatcccg aactgcgcgc ggacgctgcg gcgatgcttg cgcagcgccg
      361 gcgagaaggc gtcggaggcg acggcgttgt agatgacgtg caggcgcgaa agcggcacgc
      421 cgaagcggcc ggcgatgtcg tccttcacca tctgcgaatt gcagatcacc gcgcgcagcc
      481 gcgggctcgc gaagagccgc cgctcggccg cgagcgtgta gcggtgcgca gggttgcatg
      541 cgatgccgag ccggccgacc ggcccgagcg cccggcgacg gttctccagc cactgcgcgt
      601 gcacgccgtc gccggcgcgg tagatgtcgc agcaggcgat gcgctcgtgc gactgcacca
      661 ggtcgaagcg gcgcgcggcg agcgcgcggc acaccgcgcg ctcgaagccc gcgtcgcgcc
      721 agacgcggcc gaggtagaac gggtcgcagc gcacgacctc gatgcccgcg cgcgcgcccg
      781 tccagcgccg cgcgaagacg ctgaacgtcg cgccgccgcc ggggaaggcg ccgagaacgc
      841 gctcgatgaa gcgctcggca ccgccgtggg ggttgtagcg ctgccggacg agcgcgatgc
      901 gcacggtcgc gcctagccgc gcgcccggtc gagcgcgatg cacagctcgc gcgcgccgtc
      961 gagcagccgc gggccgggac ggtgcaggtc gttcgcgtcg accgcgtata cgcggtgggt
     1021 gcgcaccgcg ggcacggcgt cgagtccgct ccaggcgtcg cgctcggccg cggacccggt
     1081 gacaacgacc tccggccggg cgccgagcag ctgctcgcgc gacacgaccg gcgtcgcagt
     1141 gcgcgcttgc gcgaagacgt tgcgcccgcc gcagagctcg atcgcctcgc tgaacacgtg
     1201 ctcgccgttg atcgtgatca tgggcgtgtg ccagacctcg aggaaaacgg agagctccgg
     1261 ccgcgccgcg tactccgcgc gcagcgtcgc gagccgccgt tcgaaatcgc gcgccgcggc
     1321 gccggcgctg gccccggtgc cggcgatgcg gccgacggtg cgcagcagcg cggcgacgtc
     1381 ggcgaagtgg cgcacctcgg tgaccacgac cttgaggccg aggcgctcga gccgctcgac
     1441 ctgcagcgcg ggcgtgccgc tgcgccaggc gaggacgacg tcgggcctga gctggatcac
     1501 gcgctcgtaa tcgacgcggc cccagtcgaa gacgaccggc aggcgcttcg ccgcgggcgg
     1561 gaagtcgctg aagttggaca cgccgaccag ccggtcgccc gcgcccgccg cgtacgcgat
     1621 ctcggcgagg tgcggcgcga gcgcgacgat gcggcgcgcc ggcgcgtcga gcacgaccgt
     1681 cacgccgcgg tcgtcgcgca cctcgatggc tgtcgcggcg gcgtgcgccg aggcgagcag
     1741 cgtcagcgcc gcgacgagcc cgcgcgcgag cctcatcgca gcagcgcgtc caccgccccg
     1801 agcaccgacg cgagcggcag gtgcgtgagg cactcgctca ccttgccgcc gccgcagccg
     1861 tcgttgccgc acggccggca cggatgcacg gtcgacgcga ccacgcggtg cgcgacctgc
     1921 caaggccccc actcgagctc gccgctcggg ccgaacaggg cgacgacggg cgtctgcatc
     1981 gccgcggcga tgtgcatggg cgcggagtcg acgccgacga agagcctggc gcgcccggcg
     2041 agcgccgcga gctccttgag cgtcagctgc cccgagaggt cgagcggctt cacgcgcgtg
     2101 cgcgagagca tgtcgtcgac gagctcgcgc tcggcgggat cgggcgcggc ggtgagaacg
     2161 acccgatgcc cgcgccgctc cagctcgtcg agcagcgccg cggcccggtc ggcgggccag
     2221 cacttgaact gccagcgcga gcccgggtgg aaatgcacga agcccttcgc cgcgaggccg
     2281 gcctgcgcga gcagcgcgtc gacgcggcgc tcggccgcct cgccggggac gaggacgagg
     2341 cgccgctcgt cgtcgcccgg ccggatgccg agccggcgca gcgcgtcgag gttcaactcg
     2401 accatgtggc ggcgcgcgcc tttcggcagc gcgtagcggt gcgtaaagct gcgcttccag
     2461 tagcgcggct tgtcggcgta gtcgggcgcg accgcccagc gcgcgccgag cgcgcgcgcg
     2521 agccacgcgc cgcgcggatg ctcggagagg tgcacgacga ggtcgtagcg gcgcgcgcgc
     2581 agccgcgaga acagcgcgag ctccgcgcgc gcctgcgcga gcgcgcccga tcgcttccag
     2641 gcgcggtcga tcacgtggat ctcggcgatc gccggatgca gggacagcat gtcgcgcgta
     2701 tcggcgtaga ccagcgcgtc gatctcgatt cgcggcgcga ggttcttcag caccgagaac
     2761 acgggcgacg cgagcagcac gtcaccgtgg tggcgcagct tgatgacgag cgcccgcgag
     2821 agcgcgccgg ggtcgatcgc gtcggcgggt ccgtagggca tggcgggcaa gatagcagag
     2881 aacccggcgg cggccaaagc aggcgcgcag cgcgcgcgct acgacgacgc gatgtggcgc
     2941 ggtgtctcga cgcggacgag gtcgctcgcc gcggagcgcc gcggaaagat ctccgggtgg
     3001 atgatttcgg ccatgatctc caggctgtcg acgagcctgg gtccgggccg gctgaaataa
     3061 gcgcagccat tgaccgcgta gacgcggtcc tcgcggaccg ccggcaggga gtcccacgcg
     3121 cggcgcgact gcaggatgcg caggtcggcg agggtgcgct cgatctcgta gccgcagcac
     3181 gcgagcacca ggatatcggg ggccgcttcg acgagcttgt cccaggcggt gcgcaccgag
     3241 ggcttgccct tgaggccgag cggatcgacg ccccccgcga tctcgaccag ctcggggctc
     3301 caatggccgc tgcagtagag cggatcgatc cattcgagct gcacgcaccg gggccgggat
     3361 tgggcgcgcg aggccttggc gtgcacggcc tcgacgcggc gctcgagcga ctcgaccagc
     3421 gcgtcgccgc gctcgggcgc gcccagcgcg gccgcgacgc ggcggatgtc atccagcacg
     3481 tcggcgaagg acgagggcga gaggctcacg agctgcggct tgccggggag cgagtgcgcg
     3541 atcgcggaga tcgtgcggta atcgatggcg cagacgtcgc acagctgctg cgtgatgatc
     3601 acatccggcg ccaggcggcg cagcagcggc tcgtcgatcg tgtacagcgt gccgttcgac
     3661 gagagctcac cgctcaccca gcggtcgacc tccgcgctcg gcaggccctt gccgtagacc
     3721 tcgcaatgcg tgacgcgcgg ccggccgttg acctcgggcg gatagttgca ctcgtgcgac
     3781 acgccgacca gcgagtcggc gaagccgagc gccgcgacga tctcggtcgc cgcggggagc
     3841 agcgagacaa tgcgcatgcc gctaggccgg cgcgcgcccg gcgcgcgagc gcacgagatc
     3901 gaacgcgccg aagagcacgg cggtccagaa caggtccccg gcgagcccgt tgcgcaggaa
     3961 cggcagtccc atccagtagc tctcggcgag gccgtcgagc gtaggcggat agagcggcgc
     4021 gtggttgccc gggagtgccc agaccgcgaa gttggtcacc agatagaaca ggagcgaccc
     4081 gccgaccgcg gcgaagccgg tccgcgtcca agtcctttgc gtcgccagcc agcggccgat
     4141 gccggtgccg gctaggaacg cgccgtagac cacggccgtc atccatccga aagaattccc
     4201 gagcgcgagg tcgctcagga acagcgcggt caccgggacc gcgaacgcgg ccgccccggg
     4261 aagcatcgcg ccgccgaaca gggcgatggc gagcaccgga gtgaagttgt cggggtgcgg
     4321 caggaggcgc ccggccagcg ccatgacgat cagcaggacc acgagcccgt gtttcatcgt
     4381 cgtctccttt tcagtgcgtg gttttcatgg tgtcgcggca gcgctcgacg aagcgctccg
     4441 ccaatgccgg ctgggcggcg aagtgcgcgt gcacgtagct cgcgagcagg ttgccctcgg
     4501 cgcagccatc caacgtggtc ttgccgccat gatacagctt gagcgccggc gcgaagcgcg
     4561 cgtcctcgag caccgaatgg tggaaggtct gcgcgcggta gcggtccccg gcgcgcgcga
     4621 gcatggtgtc gcgcagcgcc tcgacctcga cgtacccgag agacagctga tcacgccgca
     4681 tcaccgaggt cgccggcacg agacctgcca tcgcgtggcg ccgtccctcg agatccacca
     4741 gcgcatcgca gaggatcatc aagccgccgc actccgcgta tatcggcccg ccggccgccg
     4801 cgaagacgcg gatcgcgtcg agcatcgtcg cgttcgcggc gatcttctcc gcgaacagct
     4861 cggggaaccc gccgccgaga tacaggccgt gcgcaccgcc cggcagccgg tcgtcgtcga
     4921 gcacgctgaa cggggcgatc tgggcgcctt tgcacgcgag caggtcgagg ttctcctggt
     4981 aatagaagtg gagcgccgcg tcgcgggcga cggcgatgcg caccggcgcg ccgccgcggc
     5041 gctcggcgac gcgatcgggc gccccggtct ccggcggccg cggcgcggcg cgc
//
LOCUS       DATDMW010000145         4181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_44390, whole genome shotgun sequence.
ACCESSION   DATDMW010000145 DATDMW010000000
VERSION     DATDMW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44390"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VI319_06410"
     CDS             <1..490
                     /locus_tag="VI319_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6863516.1"
                     /translation="PVWETRAVSFPLNVDACASCHAYSAALHDRAPPADETHGGKVKR
                     EDYAAGPYAKHDVAPRLADATPLERRGEALFRKNCAFCHAADGSGRNWIGTFMEPHPR
                     DLADPEFRRAATRERIARAVREGLPGTSMPAWKSVLAPPDIDAVVAYVARGFGPYGDR
                     AD"
     gene            572..2446
                     /locus_tag="VI319_06415"
     CDS             572..2446
                     /locus_tag="VI319_06415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6863517.1"
                     /translation="MRLLLINPRYPESFWSFKWAVDSVLPGKRAINPPLGLATLAALC
                     PRHWHVEIVDENVEPVPLDPEADIVGVCGMGVQHERQQALLDFYRARGHTVVAGGSYA
                     SLCPEQYAGRADSVIAGEAETIWPEFCRDFEAGCAKALYRETGVVALADSPVPRFDLL
                     KLDRYTTATLQFSRGCPFLCEFCDIIVMFGRKPRHKTPGQVGRELDALRALGATNLFF
                     VDDNLIGNKPVARELLRFLADYQARHGHPFNFGTEVSLNLAQDAELLALFRAAGFTWV
                     FTGIESPDPETLKATRKIQNTKEDPLVSVRKLYAHGIDVLAGFIIGFDRDTEATFDVQ
                     HDFIVESGIQAAMAGLLTALPRTPLYERLEQEGRLRRDTGHSDNTKLATNVVPKNMTY
                     DRMVALYRRLHQRLASDAGIARRIRNKMRYMADPVYRGEYSGAAQAGIVAKLFFRGIL
                     PGGPGRIAAFLTSVPWTAPRKIPLAIVDWIAGLAMHSYVERHFHVAASRDSALVRVRR
                     ALRRAAARHAPEGRIALALRRLGTAASVSLRIRGEVPNEYFRRIARPIERLLANGRSR
                     LDLHVDDLCDPGADALRELLVRLRRHGDRVSIFVAEKWRAAVAVDSSVFHHRLQPAEG
                     "
     gene            complement(2463..2816)
                     /locus_tag="VI319_06420"
     CDS             complement(2463..2816)
                     /locus_tag="VI319_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863518.1"
                     /translation="MKARRASIGDLPVAPTTVTDASEPIGFPRVHAAIKWAIVVALVL
                     MAILAAAFWALILGGFAASGFHASDPAGGQAEVVITPECAWPYSVDDHDAQAVCRMFY
                     RLTPEQRAQVLKARK"
     gene            complement(2813..3001)
                     /locus_tag="VI319_06425"
     CDS             complement(2813..3001)
                     /locus_tag="VI319_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863519.1"
                     /translation="MKPFTTIAVVFLAAIAVLHLFRLLAGWEVSVVGFVIPVWWSVIG
                     FAVPGGLAFMVWREHARA"
     gene            complement(2998..3471)
                     /locus_tag="VI319_06430"
     CDS             complement(2998..3471)
                     /locus_tag="VI319_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6863520.1"
                     /translation="MTVKRMDNVGIVVEDLDVAIGFFTELGLELEGRAPIEGDWADGI
                     TGLRDMRVEIAMMRTPDGHGRLEMSRFLAPPAVADHRSAPVNALGYLRVMFTVEDIDD
                     TLARLGKRGAKLVGEVVRYRDVYRLCYIRGPEGILIGLAQELGQHAYQSNAEGPT"
     gene            complement(3583..>4181)
                     /locus_tag="VI319_06435"
     CDS             complement(3583..>4181)
                     /locus_tag="VI319_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863521.1"
                     /translation="RADDKRHALIYLDQRTVMHTIHVRPHMTLGDKLKALLALGKSAG
                     ASATGGDPLDYDQVVSLDAEDLAEQGILAAYQALLPQLRQYAASPLELTETIDNDAAM
                     YSVCAGDQKFEIWEAGAKNPDGWERATVAFFQIVNASLTSSPYKFYALYGGNDLSGIF
                     LTEVEFAAARRAIKEPSNRPWLPVDEPPHYGYPVAGAV"
BASE COUNT          675 a         1454 c         1383 g          669 t
ORIGIN      
        1 gcccgtctgg gagacgcgcg cggtgtcctt cccgctcaac gtcgacgcct gcgcgagctg
       61 tcatgcgtac agcgcggcgc tgcacgaccg cgcgccgccc gcggacgaga cgcacggcgg
      121 caaggtgaag cgcgaggact acgccgccgg tccctacgcg aagcacgacg tcgcgccgcg
      181 actcgcggac gccacgccgc tcgagcgccg cggcgaggcg ctgttccgga agaactgcgc
      241 gttctgccac gcggccgacg gcagcggccg gaactggatc ggcacgttca tggagccgca
      301 tccgcgcgac ctcgccgacc cggagttccg gcgcgcggcg acgcgcgagc ggatcgcgcg
      361 cgcggtgcgc gaggggctgc cggggacttc gatgccggcg tggaaatcgg tgctcgcgcc
      421 gcccgacatc gacgccgtcg tggcctacgt cgcgcgcggc ttcgggccgt acggcgatcg
      481 cgccgactga ctccgccgcg cgtgacccgg gtcacagccc gggcggcggg cgcagactac
      541 attcgacttc cttctccgag gagcgagcgc gatgcgcctc ctgctgatca atccccgcta
      601 tcccgagagc ttctggtcgt tcaagtgggc ggtcgattcc gtgctgcccg gcaagcgcgc
      661 gatcaacccg ccgctcggcc tggcgacgct cgccgcgctg tgcccgcggc actggcacgt
      721 cgagatcgtc gacgagaacg tcgagcccgt gccgctcgat cccgaggccg acatcgtcgg
      781 cgtatgcggc atgggcgtgc agcacgagcg ccagcaggcg ctgctcgact tctaccgcgc
      841 gcgcggccac accgtggtcg cgggcggcag ctacgcgtcg ctgtgccccg agcagtacgc
      901 ggggcgcgcg gacagcgtga tcgccggcga ggccgagacc atctggcccg agttctgccg
      961 tgatttcgag gcgggatgcg cgaaggcgct ctaccgagag accggcgtcg tggccctcgc
     1021 cgactcgccg gtgccgcgct tcgatcttct caagctcgac cggtatacga ccgcgacgct
     1081 gcagttctcg cgcggctgcc cgttcctgtg cgagttctgc gacatcatcg tcatgttcgg
     1141 ccgcaagccc cgccacaaga cgcccgggca ggtcgggcgc gagctcgacg cgctgcgcgc
     1201 gctcggggcg acgaacctgt tcttcgtcga cgacaacctg atcggcaaca agccggtcgc
     1261 gcgcgagctg ctgcgcttcc tcgccgacta ccaggcgcgg cacggccacc ccttcaactt
     1321 cggcaccgag gtctcgctga acctcgccca ggacgcggag ctgctcgcgc tgttccgcgc
     1381 cgcgggcttc acctgggtgt tcaccggcat cgagtcgccc gaccccgaga cgctgaaggc
     1441 gacgcgcaag atccagaaca cgaaggagga cccgctcgtc tcggtgcgca agctctacgc
     1501 ccacggcatc gacgtgctcg cgggcttcat catcggcttc gaccgcgaca ccgaggcgac
     1561 cttcgatgtg cagcacgact tcatcgtcga atccgggatc caggcggcga tggcggggct
     1621 cctcaccgcg ctgccgcgca cgccgctcta cgagcggctg gagcaggaag ggcggctgcg
     1681 ccgcgacacc ggccacagcg acaacaccaa gctcgcgacc aacgtcgtgc cgaagaacat
     1741 gacctacgac cgcatggtgg cgctgtaccg gcggctgcac cagcggctcg cctccgacgc
     1801 cggcatcgcg cggcgcatcc gcaacaagat gcgctacatg gccgatcccg tataccgcgg
     1861 cgagtactcg ggcgcggcgc aggccgggat cgtggcgaag ctcttcttcc gcggcatcct
     1921 cccgggcggg ccgggacgga tcgcggcgtt cctgacatcc gtgccctgga ccgcgccgcg
     1981 caagatcccg ctcgcgatcg tcgactggat cgcgggcctg gcgatgcaca gctacgtcga
     2041 gcggcacttc cacgtcgcgg ccagccgcga ctccgcgctc gtgcgcgtgc gacgcgcgct
     2101 gcgccgggcc gcggcgcgcc acgcccccga aggccggatc gcgctcgcgc tcaggcgctt
     2161 gggcaccgcg gcctcggtgt cgctgcgcat ccgcggcgag gtgccgaacg agtatttccg
     2221 ccgcatcgcg cgcccgatcg agcgcctgct cgcgaacggc cgctcgcgcc tcgacctgca
     2281 cgtcgatgac ctgtgcgacc ccggcgccga cgcgctgcgc gagctgctcg tccgcctgcg
     2341 ccggcacggc gaccgcgtgt cgatcttcgt cgcggagaaa tggcgcgcgg cggtggcagt
     2401 cgactcgtcg gtgttccacc accggctgca gccggcggag ggctgatcgc tcagcgaggg
     2461 ttttacttgc gcgccttcag gacctgggca cgttgctcgg gcgtcaggcg gtagaacatc
     2521 cggcatacgg cttgagcgtc gtgatcgtcc acgctgtaag gccacgcgca ctcgggagtg
     2581 atcaccacct cggcttgtcc acccgccggg tcggatgcat gaaatccgga agcggcgaat
     2641 ccacccagga tcaatgccca aaatgccgcc gccaggatcg ccatgagcac cagtgcgacc
     2701 acgatcgccc acttgatcgc ggcatggacc ctcggaaacc cgatcggctc ggaggcgtcc
     2761 gtgaccgtcg tcggcgctac cggcaagtcg ccgatgctcg cacgccgggc tttcacgctc
     2821 gcgcgtgctc gcgccagacc atgaacgcga ggccacccgg aacggcaaag ccaatgacgc
     2881 tccaccagac cgggatgacg aacccaacga cactgacttc ccagcccgcg aggagccgaa
     2941 acaaatggag aacggcgatt gccgcgagga aaacgacagc gatggtcgtg aacggcttca
     3001 tgtgggtccc tcggcattcg attggtaagc gtgctgcccg agctcctggg cgagcccgat
     3061 gagaattcct tcggggcccc ggatgtagca gaggcgatac acgtcccggt accggaccac
     3121 ttcgccgacg agcttcgcgc cgcgtttccc gagccgggcg agcgtatcgt cgatgtcctc
     3181 gacggtgaac atcacgcgca ggtaaccgag agcgttcacc ggagcgctgc ggtgatcggc
     3241 gaccgcgggc ggcgcgagga atcgggacat ctcgagccgg ccgtgaccgt ccggcgtccg
     3301 catcatggca atctcgacgc gcatgtcgcg caatccggtg atgccatcgg cccagtctcc
     3361 ctcgatcggg gcgcgcccct cgagctcgag cccaagctcg gtgaagaacc cgatggcgac
     3421 atcgaggtcc tcgaccacga tgccgacgtt gtccatgcgc ttgaccgtca tggcgtctaa
     3481 cgtcggagtt gaccggcacg tagcggcatt gcgccgcacg gcgtacgctg accagcacgc
     3541 cttgcggcgc aatgccactg cgtgtccgcg tcgaacgacc tgttagaccg caccggcgac
     3601 cgggtaccca taatgcggtg gctcgtcgac tgggagccaa ggccgattgg atggctcctt
     3661 aatcgctcgg cgggcagcgg caaattcaac ttcggtgagg aatatgccgc tcaggtcatt
     3721 gccaccgtaa agagcgtaga acttgtacgg cgagctagtg aggctggcat tcacgatttg
     3781 gaagaacgcc accgtcgcgc gctcccagcc gtctgggttc ttcgctcctg cttcccatat
     3841 ttcgaacttc tgatctccag cgcagactga gtacattgcg gcatcgttgt caatggtctc
     3901 ggtgagttcc aagggagatg cagcgtactg cctcagctga gggagcagtg cttggtaggc
     3961 agcgaggatt ccctgttctg caagatcctc tgcgtccaat gacaccactt ggtcgtaatc
     4021 caatggatcg ccgccggtgg cggatgcgcc agcggacttg ccgagcgcca gtagtgcctt
     4081 aagtttgtcg ccgagtgtca tgtgcggtct aacatgtatg gtgtgcatca ctgtcctttg
     4141 atcaaggtat atcagggcgt gtcttttgtc atcggccctc g
//
LOCUS       DATDMW010000146        15679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_292212, whole genome shotgun sequence.
ACCESSION   DATDMW010000146 DATDMW010000000
VERSION     DATDMW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15679
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292212"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1140)
                     /locus_tag="VI319_06440"
     CDS             complement(148..1140)
                     /locus_tag="VI319_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY6863522.1"
                     /translation="MAWWLIACAALIYVMVVVGGVTRLTHSGLSIVEWQPIVGAVPPL
                     DAAQWQEAFDQYRKTPEYLQVNRGMTLAQFKDIFWWEYAHRLLGRLIGVVFLVPLAWF
                     ALRGRVPRALLPRLAAIFVLGGLQGALGWYMVQSGLVDDPRVSQYRLAAHLGLAAAIY
                     AAIVWTALDLLRAGAPRPPAPAGLRRFALALVGIVFVMMLSGALVAGIRAGLAYNTFP
                     LMDGHVLPPESFALEPWFRNLFSNMALVQFDHRLVAWTLAGLVPWFWVRSRRGAPSPL
                     ARRAGDALLGLFILQIALGISTLLLRVPVPLAAAHQAGALAVLTAALVAAHALR"
     gene            complement(1160..1534)
                     /locus_tag="VI319_06445"
     CDS             complement(1160..1534)
                     /locus_tag="VI319_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEY6863523.1"
                     /translation="MEFATLRAVHVAAVAASGLMFFVRGLWMLARSPMLERRWVRIVP
                     HLNDTVLLVAGAWMAVLLRQVPGVSPWLTAKLVALVIYVALGAVALRRRRVGAWLAAL
                     AVFFYIVAVALTHDALPWRAWR"
     gene            1599..2030
                     /gene="trxC"
                     /locus_tag="VI319_06450"
     CDS             1599..2030
                     /gene="trxC"
                     /locus_tag="VI319_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEY6863524.1"
                     /translation="MAEPVHGVCPHCAATNRVPGDRLDERPVCGKCKQALFTGRPVEL
                     DADAFERHVGANDIPVVVDFWAAWCGPCRMMAPQFERAAADLEPRARLAKLDTEAEQA
                     IAARYGIRSIPTVIAFRGGREVARQSGAMDSGTLVRWVRGL"
     gene            complement(2027..3004)
                     /locus_tag="VI319_06455"
     CDS             complement(2027..3004)
                     /locus_tag="VI319_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY6863525.1"
                     /translation="MQLFAAGRRLEQVDAAPASPDAGQILVRVHACGVCRTDLHIVDG
                     ELPAPAHPVVPGHEIVGTVEAAGAGVERFRAGDRVGVPWLGWTCGRCAYCRSGRENLC
                     DGARFTGYHIAGGYADYALADARYCFALPAGYDDARAAPLLCAGLIGYRALRMAGDAD
                     RLGLYGFGAAAHIVAQLARHEGRKVFAFTRAGDAASQRFALGLGAAWAGASTEAPPEP
                     LDAAILFAPVGALVPAALRAVAKGGTVVCAGIHMSDVPSFPYSILWGERRIVSVANLT
                     RADGEAFLALAPKARVVTSVETIPLEQANEALARLREGRVEGALVLTTG"
     gene            3107..3343
                     /locus_tag="VI319_06460"
     CDS             3107..3343
                     /locus_tag="VI319_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863526.1"
                     /translation="MFAIVYKADGVPICVQVAGVSPDPVVVWNSEGDAKAFIEAKGGS
                     ADYKPVAVDEKSMEAMAKALGCPVEKLMVEPYPS"
     gene            complement(3359..4183)
                     /gene="panC"
                     /locus_tag="VI319_06465"
     CDS             complement(3359..4183)
                     /gene="panC"
                     /locus_tag="VI319_06465"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY6863527.1"
                     /translation="MLHSVAALRERLQRAPDNVFVPTMGNLHDGHIELMRLAKQRAEC
                     VVVSIFVNRLQFGPKEDFDRYPRTLQADADKMRAVGVDVVFAPDEREIYPEPQTFVVE
                     PSDLQHILEGAFRPGHFRGVATVVLKLFNMVRPRAALFGKKDYQQYVVLRDMVKQLAL
                     PLEIMPVDTVRAEDGLALSSRNGYLSPVERKEAPRLYQTLQVARAEIAAGKRDFQRME
                     LQAMSTLAWHGWRPDYISVRRQSDLQPPAPGDRELVVLAAAVLGRTRLIDNVEITL"
     gene            complement(4189..4983)
                     /gene="panB"
                     /locus_tag="VI319_06470"
     CDS             complement(4189..4983)
                     /gene="panB"
                     /locus_tag="VI319_06470"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY6863528.1"
                     /translation="MSRLSITAMQKMRDDGERIAMLTCYDASFAAALDAAGVDSMLIG
                     DSLGMVLQGHDTTLPVKLADVAYHTAAVVRGSKRAFIIADLPFGTYQESPAQAFASAA
                     QLLAAGAQMVKLEGGAEFSDTVRFLAARSVPVCAHLGLTPQSVHAFGGYRVQGRGAEA
                     ERRLIEDAVELEAAGAGMLVLEAIPAKLGEQVTKRLKIPTIGIGAGVQCSGQVLVLHD
                     MLDVFPGKKAKFVRNFMEGASSIADAVTRYVRAVKNGSFPAEEHTF"
     gene            complement(5015..5656)
                     /locus_tag="VI319_06475"
     CDS             complement(5015..5656)
                     /locus_tag="VI319_06475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY6863529.1"
                     /translation="MSLSKYRYIVVEGPIGVGKTSLARRLAERLDSALLLERPELNPF
                     LPRFYQDMDRFALPTQLFFLFQRVQQLRELAQLDLFRRVTVSDFLLEKDPLFARLTLS
                     GDELHLYQQIYDSLKPQAPKPDLVIYLQAQPATLWERVRRRGAEFERPITPEYLAMLA
                     DSYARFFYHYGGGPVLIVNSERLNFVRQDEDLDLLVERVDAMRGGREFFNWGE"
     gene            complement(5754..6440)
                     /locus_tag="VI319_06480"
     CDS             complement(5754..6440)
                     /locus_tag="VI319_06480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6863530.1"
                     /translation="MLALIGKLGEYLRTDGVWRTSLRALGHAVYLHNRWIDRGFDRRF
                     GTDTCGVIPPEELDLGGEHAAGANRYEPVQVPAFRRIMRDLPIRYEDHTFLDLGSGKG
                     RALLMASRVPFRRVIGVELSPAMHAAAARNVAIFERRHPEAARIELVHGDALHYPLPE
                     GNLVCFFYNSFGCPLVAKMLARVEAATRDRQRHIFVVYRNPVCADLFDGASFMHTVAH
                     HPAYRIYRTR"
     gene            complement(6515..6862)
                     /locus_tag="VI319_06485"
     CDS             complement(6515..6862)
                     /locus_tag="VI319_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEY6863531.1"
                     /translation="MRATVLLQTVGLLTLSNVFMTFAWYAHLKELSAKPWFVAALVSW
                     GVALFEYLLQVPANRIGFTQYSLAQLKIVQEAVTLSVFVPFALLYMNQPLKLDYLWAS
                     LCIMGAVYFIFRA"
     gene            complement(6867..7349)
                     /gene="folK"
                     /locus_tag="VI319_06490"
     CDS             complement(6867..7349)
                     /gene="folK"
                     /locus_tag="VI319_06490"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY6863532.1"
                     /translation="MAEKAFIGLGANLGDPGEQLRGALAALDAIPGTRLTRASSLWRT
                     APVGHAAQPDFVNAVAEVETTRAPRALLDALLAIEERFGRERSFANAPRTLDLDLLLY
                     GDRVLSEHGLEVPHPRMHERAFVLAPLVEVAPDVEIPGKGRADALLARCRDQRIEKTG
                     "
     gene            complement(7366..8553)
                     /locus_tag="VI319_06495"
     CDS             complement(7366..8553)
                     /locus_tag="VI319_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6863533.1"
                     /translation="MRRLLALWKKEWLALARDVHGLAVLFLMPAAFIVIMSLALSDAF
                     KDDAGRSTDFAVLGTEDAVLAERLAQSLAGGGFRATPAPADEGAARDSVRRGSRGLVL
                     VVPPGFGRTLETVPDPKVQAPALVLLADPTLPPTQLVAFQQRVLGATLGARMSALATG
                     AGFSSDPGAFDLKRAATLNVEVVGNPRSGRPSSVQQNVPAWLIFGMFLVVMPISSLFI
                     VERREGTLARLVSQQVPFSMLLLGKVGPFFVVNLLQTALMLLAGRYVVPWFGGEALVV
                     PPSWGLLAAVSACTSLAAIGWGLAVAVFTRTAEQAIVLGGVGTILVAAMGGIMVPRFV
                     MPESMQSVVDLSPMSWALQAFHAVILRQGGWSDIALPCAKLLALGAALLALALLVHHR
                     RRV"
     gene            complement(8565..9488)
                     /locus_tag="VI319_06500"
     CDS             complement(8565..9488)
                     /locus_tag="VI319_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863534.1"
                     /translation="MLSIEGLSFRYPRGDADVLEDVALEIPAGGIFGLLGPNGAGKTT
                     LISIVSGQLRAGAGGVAFAGEPLDALRRRRPHCLGLVPQEHAFYPMLTCAENLRFFAG
                     AQGLAGARLRERVEAVAAFARIEDVLGRRAGQLSGGLKRRLNLAIGLLTEPDIVLLDE
                     PTVGVDPQSRAFLLDSIRSLAGPGRVIVYTSHYMEEVQAICDRIAIIDRGRVLLSGTL
                     DDVLRDASNLLTLRLRAPLPKWLATEWTTRYALADDGDARYRLRLPDASAIAGTIESA
                     RAAGCEPLAVAYGERDLEQVFMQLTRRSLRD"
     gene            complement(9482..10531)
                     /locus_tag="VI319_06505"
     CDS             complement(9482..10531)
                     /locus_tag="VI319_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HEY6863535.1"
                     /translation="MQQRAMQPQDATTKQHGSFTHAHAAHCESGVMASLVRHYGFPFT
                     EAMAFGLSSALCFAYLPFVRINGLPLIAFRMWPKSIIQGLEKGLGLGMRFETFRSPER
                     GMARLDELLEEGKLVGLQTSVFWLPYFPSDLRFHFNAHNVLVCAREGDEYVISDPVAE
                     MLVRCARADLQKARFAKGMLAPKGLLYYPTRKPVAPDWARVGRRAIAKTCHVMLHSPL
                     PIIGVRGIRMLAKRVERLPVDGNGGAARMYMGNIVRMQEEVGTGGAGFRFLYASFLQE
                     LAALAGHGALEGFASRLVDIGDRWREFAVAAARQIRRRDPLAPPALAAQLRGIADHEE
                     SFFRDLRRAQQAWRC"
     gene            complement(10534..10929)
                     /locus_tag="VI319_06510"
     CDS             complement(10534..10929)
                     /locus_tag="VI319_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863536.1"
                     /translation="MRERDVPQEGNVTLAGHRKAVYAVGEDGRLKIVASRGWEVEETV
                     TRQAVEELERLAREARERALDGTGSTLEYHMYRARMDVPLLSQTTGLWRWRVKRHLRP
                     GAFERLPGAIKRRYAEAMGIPVEALEKLD"
     gene            complement(10922..12079)
                     /locus_tag="VI319_06515"
     CDS             complement(10922..12079)
                     /locus_tag="VI319_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY6863537.1"
                     /translation="MSRRTTYITRTAALLPNAPVENDAVEAILGQVGDRPSRARRLVQ
                     RSNGIRRRHYVIDPKTGEPTHSNASLTAAAVKGLVGGGFALEDIACLACGTSNPDQLL
                     PNHAVMVHGELGAPVCEVVATSGVCVSGMTALKYAWMAVASGDAKNAIATGSEVASLG
                     LRAHNYDGERQQDVAALEAQPGLAFEKDFLRWMLSDGAGAVLIEPAPRKGLNLRIEWI
                     DVFSYAHAMPACMYMGAEKEADGRLRGWMRYDVADRDRHSVFAIKQDVRLLNESVIEY
                     TVVKPLRKVLERREVTPAGVTWLLPHISSEYFRQPVADGFARAGLPLPQDKWFTNLDH
                     VGNTGSASVYLMLDELAKSGRLKEGDRLLCFVPESGRFSSSFMVLTVVNHA"
     gene            complement(12086..13003)
                     /locus_tag="VI319_06520"
     CDS             complement(12086..13003)
                     /locus_tag="VI319_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
                     /protein_id="HEY6863538.1"
                     /translation="MKRVLLVHFSQSGQLTRVARRLVEPLAAADDVELVEEALRPVAP
                     YAFPWSFFGFLDAMPESVLLDPPALEPLAVRGDEHFDLVVLAYQVWFLAPAGPITAFL
                     KSETGRRLLAGRPVVTVIACRNMWLVAQEAVKGLIADAGGVLKDNVVFTDQGGTVATL
                     VTTPRWVLTGKKNAFCGLPPAGVAEEEIAGADRFGRALLAALRAGRERDGRPMLAGLG
                     AARVDPTLIFSERAARRAFGAWSRVIRLGGRRGAATRLPFLALFCVYLVAMVIVIVPP
                     SLLVQRLLRPLLARRLASQKAYFELPSGG"
     gene            complement(13183..14577)
                     /gene="pcnB"
                     /locus_tag="VI319_06525"
     CDS             complement(13183..14577)
                     /gene="pcnB"
                     /locus_tag="VI319_06525"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEY6863539.1"
                     /translation="MIKRFISRVFRLGSGPQPVVVPREKHGLGRERISSGSRKVCEVL
                     RERGYQAYVVGGAVRDLLLGLEPKDFDVAPDARPEEIKRLFRRAIVIGRRFRLVHVMF
                     GDETVEVSTFRAPSAPEPEETLAGETHERDAAPDHQVDAHGRILRDNVYGTREDDAAR
                     RDLTVNALYYDPYSEAILDFHGGLRDLERKSVRMIGDPRGRYREDPVRMLRAARFAAK
                     LGFKIDAKTRAPIRELAPLLENVPPARVFEEMLKLLLSGHAATSVRQLRDEGLHHGLL
                     PLLDVIVEQPLGQRFVQLALEQTDERVRAGKPVSPAFLFAALLWHEVLAAWSKAEKKG
                     AKRIPALYQAMDAVVDIQVSKLSIPRRFTAIMKEIWSMQPRFEQRSGRRPFALTAHER
                     FRAAYDFLVLRASSGEVPEELVQWWTRFQHAGESERTTMLLAPGPGEPGRRRRRRRRN
                     RGGGAGQPPAAPAA"
     gene            complement(14574..15317)
                     /locus_tag="VI319_06530"
     CDS             complement(14574..15317)
                     /locus_tag="VI319_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863540.1"
                     /translation="MLAARAIGIQAGGRTLVEGLDLALAPGQCWALLGPNGAGKSSLI
                     LALAGLRAPHRGEIALDERPLARWRRSELARRVGVLLQDDTPDFWGTALEYARLGRYP
                     RSGGAWGGGAEDDDAALGWLARLDLAERARQPYVTLSGGERQRARIAQLLVQDAALLL
                     LDEPLSHLDLRHQVQVMELLAESARAGRAVLMALHQPSLAARYCDHAVLLYDAGRSSA
                     GSCADMLTPSRLEALYRCRLESAGLSIPT"
     gene            complement(15320..>15679)
                     /locus_tag="VI319_06535"
     CDS             complement(15320..>15679)
                     /locus_tag="VI319_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY6863541.1"
                     /translation="RASALDVVLLGSDKAASLGVDVARLQVIAFAAATLATVAAVLLG
                     GAIGFVGLVVPHVLRLAGVRAHRLLLPLSVCAGGSLLVVADTVARTATAPSELPVGVI
                     TALIGVPALLFLLARVR"
BASE COUNT         2418 a         5544 c         5669 g         2048 t
ORIGIN      
        1 cgtcctcgcg catgacgcgt gcgcgacgcg tgcgctcagc ttcggcgaca cgcgcgttcc
       61 cgcggagcag gtgcactgct cgttcctctc ggcgctgtcg ggatcctacg cgacggtgca
      121 gacggccgcc gagctgaaca agtccctcta gcgcagcgcg tgcgcggcga ccagcgccgc
      181 ggtgagcacg gcgagcgcgc ccgcctggtg ggccgctgcg agcggcaccg ggacccgcag
      241 cagcagcgtc gagatcccga gcgcgatctg caggatgaac agcccgagca gcgcgtcgcc
      301 cgcgcgccgc gcgagcggcg agggcgcgcc gcgccgtgag cgtacccaga accacggcac
      361 cagccccgcg agcgtccagg cgacgaggcg gtggtcgaac tgcaccagcg ccatgttcga
      421 gaacaggttg cggaaccacg gctcgagcgc gaaggactcg ggcggcagca cgtgcccgtc
      481 catgagcggg aaggtgttgt acgcgaggcc cgcgcggatc ccggcgacca gcgcccccga
      541 gagcatcatg acgaacacga tcccgaccag cgcgagcgcg aagcgccgca gcccggccgg
      601 cgcgggcgga cgtggcgcgc ccgcgcgcag cagatcgagc gcggtccaga cgatggccgc
      661 gtagatcgcc gccgcgagcc ccaggtgcgc ggcgagccgg tattgcgaga cgcgcggatc
      721 gtcgaccagg ccgctctgca ccatgtacca gccgagcgct ccctgcagcc cgcccagcac
      781 gaagatcgcg gcgagccgcg gcagcagcgc gcgcggaacg cggccgcgga gcgcgaacca
      841 cgcgagcggc acgaggaaca cgacgccgat cagccgcccg agcagccggt gcgcgtactc
      901 ccaccagaag atgtccttga actgcgcgag cgtcatgcct cgattcacct gcaggtactc
      961 gggcgtcttc cggtactggt cgaaggcctc ttgccattgc gccgcgtcga gcggcgggac
     1021 cgcgcccacg atcggctgcc attccacgat cgagagcccc gagtgcgtaa ggcgcgtgac
     1081 gccgcccacg acgaccatca cgtagatcag cgccgcgcag gcgatcagcc accacgccac
     1141 cgcgcggcgg cctgcaacgt cagcgccagg cacgccaggg gagcgcatcg tgggtgagcg
     1201 cgacggcgac gatgtagaag aacacggcga gcgccgcgag ccacgcgccg acgcggcgcc
     1261 ggcgcagcgc cacggctccc agcgcgacgt agatcacgag cgcgacgagc ttggcggtga
     1321 gccagggcga cacgcccggg acctggcgca gcagcaccgc catccaggcg cccgcgacga
     1381 gcaggacggt gtcgttcagg tgcgggacga tgcgcaccca gcggcgctcg agcatgggcg
     1441 agcgcgcgag catccacaag ccgcgcacga agaacatgag ccccgaggcg gcgaccgcgg
     1501 cgacgtgcac tgcccggagc gtggcgaatt ccatgagcgg gattttaatc gcaccgctcg
     1561 cgcttgaaac gaccgggcgc cggacccatc tgagcgccat ggccgagccc gtccacggcg
     1621 tctgccccca ttgcgccgcg accaaccgcg ttcccggtga tcgtctcgac gagcgtcccg
     1681 tctgcggcaa gtgcaagcag gcgctgttca ccgggcgccc ggtcgagctc gacgcagatg
     1741 cgttcgagcg ccacgtcggc gccaacgaca tcccggtggt cgtggacttc tgggccgcct
     1801 ggtgcggtcc gtgccggatg atggcgccgc agttcgagcg cgcggccgcc gatctcgagc
     1861 cccgggcgcg gctcgccaag ctcgacaccg aggccgagca ggcgatcgcg gcccgctatg
     1921 ggattcgcag cattccgacg gtgatcgcgt tccgcggcgg ccgcgaggtc gcgcgccagt
     1981 cgggcgcgat ggactcgggc acgctggtgc gctgggtccg cggcctctag cccgtcgtca
     2041 gcaccagagc gccttcgacc cggccttcgc gcaggcgcgc gagcgcctcg tttgcctgct
     2101 cgagcgggat cgtctccacc gacgtgacga cgcgcgcctt cggcgcgagc gcgaggaacg
     2161 cctcgccgtc ggcgcgcgtg aggttcgcga ccgacacgat gcgccgctcg ccccagagga
     2221 tggaataggg gaacgacggc acatcgctca tgtggatgcc cgcgcacacg accgtcccgc
     2281 ctttcgcgac cgcgcgcagc gccgcgggca cgagcgcgcc gacgggcgcg aacaggatcg
     2341 ccgcatcgag cggctcgggc ggcgcctcgg tggaggcgcc cgcccacgcc gcgcccaggc
     2401 cgagcgcgaa gcgctgcgac gccgcatctc ccgcgcgcgt gaacgcgaac accttgcggc
     2461 cctcgtggcg cgcgagctgc gccacgatgt gcgcggccgc gccgaagccg tagaggccga
     2521 ggcgatcggc gtcgcccgcc atgcgcaacg cgcggtatcc gatcagcccc gcgcacagca
     2581 gcggcgcggc gcgcgcgtcg tcgtagccgg cgggcagcgc gaagcagtag cgcgcgtcgg
     2641 cgagcgcgta gtcggcgtag ccgccggcga tgtggtaccc ggtgaagcgc gcgccgtcgc
     2701 agaggttctc gcggcccgac cggcagtacg cgcagcgccc gcacgtccat ccgagccacg
     2761 gcacgccgac gcggtcgccg gcgcgaaagc gctcgacgcc ggcacccgcg gcctcgaccg
     2821 tgccgacgat ctcgtggccc ggaacgacgg gatgagccgg cgccggcagc tcgccgtcga
     2881 cgatgtgcag atcggtgcgg cacacgccgc acgcgtgcac gcgcacgagg atctgtcccg
     2941 cgtccggcga cgcgggcgct gcgtcgacct gctcgagacg ccggcccgcc gcgaacaatt
     3001 gcatggaacg cgtcaaagta ccgcccgacg acgatcctgc ccggctatgg tgagggtccg
     3061 gaacatgggt tagagtagcg caacaacgga cgggaggagg gcggacatgt ttgcgatcgt
     3121 gtacaaggcc gacggcgtcc cgatctgcgt ccaggttgcg ggcgtctcgc cggaccccgt
     3181 cgtcgtgtgg aactcggaag gggacgccaa ggcgttcatc gaagcgaagg gcggttccgc
     3241 cgattacaag cccgtcgcgg tcgacgagaa gagcatggaa gcgatggcca aggcgctcgg
     3301 ctgcccggtc gagaagctga tggtggagcc ttacccgtcc tgaacgctgc agcgtccgct
     3361 acagcgtaat ttcgacgttg tcgatcagcc gcgtgcggcc gagcacggcc gcggcgagca
     3421 ccacgagctc gcgatctccg ggcgccggcg gctgcaggtc cgactgcctt cgcacggaga
     3481 tgtagtcggg ccgccagccg tgccaggcga gcgtcgacat ggcctgcagc tccatccgct
     3541 ggaagtcgcg cttgccggcc gcgatctcgg cgcgcgcgac ctgcagggtc tggtagagac
     3601 gcggcgcctc cttgcgctcg accggtgaca ggtagccgtt gcgcgagctg agggcgaggc
     3661 cgtcctcggc gcgcacggtg tcgaccggca tgatctcgag cggaagcgcg agctgcttca
     3721 ccatgtctcg cagcaccacg tactgctggt agtccttctt cccgaacagc gcggcgcgcg
     3781 gccgcaccat gttgaagagc ttcagcacca cggtggcgac gccgcggaaa tgcccggggc
     3841 ggaacgcgcc ctcgaggatg tgctgcaggt cggagggctc gacgacgaag gtctgcggct
     3901 cgggatagat ctcgcgctcg tcgggcgcga acaccacgtc gacgccgacc gcgcgcatct
     3961 tgtcggcatc ggcctgcagc gtgcgcgggt agcggtcgaa gtcctccttc gggccgaact
     4021 gcagccggtt gacgaagatc gacacgacca cgcattcggc ccgctgcttg gcgagccgca
     4081 tcagctcgat gtggccgtcg tgcaggttgc ccatcgtggg cacgaacacg ttgtcggggg
     4141 cgcgctgcag ccgctcccgc aaggccgcga ccgagtgcag gatgtccact agaaggtgtg
     4201 ctcctcggcc gggaagctcc cgttcttcac ggcgcgaacg tagcgcgtca cggcgtcggc
     4261 gatggacgag gcgccttcca tgaagttgcg cacgaacttc gccttcttcc cggggaagac
     4321 gtcgagcatg tcgtgcagca ccagcacctg ccccgagcac tgcacgccgg cgccgatgcc
     4381 gatggtcggg atcttcaggc gcttggtgac ctgctcgccg agcttcgccg ggatcgcctc
     4441 gagcacgagc atgcccgcgc ccgcggcttc gagctcgacc gcgtcctcga tgagccggcg
     4501 ctcggcctcg gcgccgcggc cctggacgcg gtagccgccg aaggcgtgca ccgactgcgg
     4561 cgtgagcccc aggtgcgcgc acaccgggac gctgcgcgcg gcgaggaagc ggacggtgtc
     4621 ggagaattcc gcgccgccct cgagcttcac catctgcgcg cccgcggcga gcagctgcgc
     4681 ggcgctcgcg aacgcctgcg cgggcgactc ctggtaggtg ccgaagggca ggtcggcgat
     4741 gatgaacgcg cgcttcgagc cgcgcaccac cgccgcggtg tggtacgcga cgtcggcgag
     4801 cttcaccggc agcgtcgtgt cgtgtccctg cagcaccatg ccgagcgagt cgccgatcag
     4861 catcgagtcg acgccggcgg cgtcgagcgc ggcggcgaag ctcgcgtcgt agcaggtcag
     4921 catggcgatg cgctcgccgt cgtcgcgcat cttctgcatc gccgtgatcg atagccggct
     4981 catggttcgc cttccctggg gtcgtgtccg gggctcattc gccccagttg aagaactcgc
     5041 ggccgccgcg catcgcgtcg acgcgctcga cgagcaggtc gaggtcctcg tcctggcgca
     5101 cgaagttgag gcgctccgag ttcacgatca agaccggccc tcccccgtaa tgataaaaga
     5161 accgggcgta gctgtcggcg agcatcgcga ggtactcggg cgtgatcggg cgctcgaact
     5221 cggcgccacg ccgccgcacc cgctcccaca gcgtcgccgg ctgcgcctgg aggtagatga
     5281 cgaggtcggg cttcggcgcc tgcggcttga gcgagtcgta gatctgctga tacaggtgca
     5341 gctcgtcgcc cgacagcgtg aggcgcgcga acagcgggtc cttctcgagc aggaagtcgc
     5401 tcaccgtcac gcgcctgaac agatcgagct gcgccagctc gcgcagctgc tggacgcgct
     5461 ggaacaggaa gaacagctgt gtcggcagcg cgaagcggtc catgtcctgg tagaagcgcg
     5521 gcaggaacgg gttcagctcg ggccgctcga gcagcagcgc cgaatcgagc cgctcggcga
     5581 ggcgccgcgc gaggctcgtc ttgccgacgc cgatcgggcc ttcgacgacg atatagcggt
     5641 atttcgagag cgacatgctg tccgaagctt gcggaagacg cgccatcata cacaagcggc
     5701 ccctcgggtt cgcggggcga gcgcggcggg cttcgggccg ccggcctgcc ggatcagcgc
     5761 gtgcggtaga tgcggtacgc ggggtggtgc gcgacggtgt gcatgaagga cgcgccgtcg
     5821 aacaggtccg cgcacaccgg gttgcggtag acgacgaaga tgtggcgctg ccggtcccgg
     5881 gtcgcggcct cgacgcgcgc gagcatcttc gcgaccagcg ggcagccgaa ggagttgtag
     5941 aagaagcaga cgaggtttcc ttccgggagc gggtagtgca gcgcgtcgcc gtggaccagc
     6001 tcgatccggg ccgcttccgg gtggcggcgc tcgaagatcg cgacgttgcg ggccgcggcg
     6061 gcgtgcatcg cgggcgagag ctcgacgccg atcacgcggc ggaacgggac ccgcgacgcc
     6121 atcagcagtg ctcgcccctt gcccgagccg agatcgagaa aggtgtggtc ctcgtagcgg
     6181 atcggcagat cgcgcatgat gcggcggaac gcggggacct gcaccggctc gtagcggttc
     6241 gcgccggcag catgctcgcc gccgaggtcg agctcctcgg gcgggatcac gccgcaggta
     6301 tcggtgccga agcgccggtc gaagccgcgg tcgatccagc gattgtggag gtagacggcg
     6361 tggccgagcg cgcgcagcga cgtgcgccac acgccgtccg tgcgcaggta ctcgcccagc
     6421 ttcccaatca gcgcgagcat gccccgattg ttcccggtca aagtgaccgt tcggcggcgc
     6481 cccgcgtggc cagcttggcc tgagccgtcc ggcctcaggc gcggaagatg aagtacaccg
     6541 ctcccatgat gcaaagggac gcccagagat agtccagctt gagcggctgg ttcatgtaga
     6601 gcagcgcgaa gggcacgaac accgacagcg tgaccgcctc ctggacgatc ttgagctggg
     6661 ccaggctgta ctgcgtgaag ccgatgcggt tcgccgggac ctgcagcagg tattcgaaca
     6721 gcgccacgcc ccaggagacc agggccgcga cgaaccaggg cttggcggag agctccttga
     6781 ggtgcgcata ccaggcgaaa gtcatgaaca cgttcgagag ggtgagcaga ccgacggtct
     6841 gcagcagaac ggtggctctc atggggtcat ccggtttttt cgattcgttg gtcccggcac
     6901 cgcgcgagga gcgcgtccgc gcgtcccttc ccggggatct cgacgtccgg ggcgacctcg
     6961 accagcggcg cgagcacgaa cgcgcgctcg tgcatgcgcg ggtgcggcac ctcgaggcca
     7021 tgctccgaaa ggacccggtc gccgtaaagg agcaggtcga ggtcgagcgt gcgcggcgcg
     7081 ttggcgaagc tgcgctcgcg accgaagcgc tcctcgatcg caagcagcgc gtcgagcagc
     7141 gcgcgcggcg cccgcgtggt ctcgacctcg gcgaccgcgt tgacgaagtc tggctgcgcc
     7201 gcatggccca ccggcgccgt gcgccataag gaggaggcgc gcgtcaggcg cgttccaggg
     7261 atcgcgtcga gcgcggcgag cgcgccccgc agctgctccc cggggtcgcc gaggttggcc
     7321 ccgaggccga tgaacgcctt ctccgccatg ggcggattct agccgtcaca cccggcggcg
     7381 gtgatggacg aggagcgcga gcgcgagcag cgccgcgccg agcgcgagca gcttggcgca
     7441 gggaagggcg atgtcgctcc agccgccctg gcgcaggatc acggcgtgga aggcctgcaa
     7501 agcccaggac atcggcgaga gatcgacgac cgattgcatc gattccggca tcacgaagcg
     7561 cggcaccatg atgccgccca tcgccgcgac caggatggtg ccgacgccgc ccagcacgat
     7621 cgcctgctcg gcggtgcgcg tgaacacggc gaccgcgagc ccccagccga tcgccgcgag
     7681 gctcgtgcac gccgacaccg cggcgagcag cccccagctg ggcggcacga ccagggcctc
     7741 gccgccgaac cacgggacga cgtagcgccc ggcgagcagc atcagcgcgg tctgcagcag
     7801 gttgacgacg aagaacggcc cgacctttcc gagcagcagc atcgagaagg gcacctgctg
     7861 cgacaccagg cgtgcgaggg tgccctcgcg ccgctcgacg atgaacagcg acgagatcgg
     7921 catgaccacg aggaacatgc cgaagatcag ccacgcgggc acgttctgct ggaccgacga
     7981 ggggcgcccc gagcgcgggt tgccgacgac ctcgacgttg agcgtcgcag cgcgcttcag
     8041 gtcgaaggcg cccgggtcgg acgagaagcc cgcgccggtc gccagcgccg acatgcgcgc
     8101 gccgagcgtc gcgccgagca cgcgctgctg gaaggcgacg agctgcgtcg gcggcagcgt
     8161 cgggtccgcg agcagcacca gcgccggcgc ctgcaccttg gggtcgggca cggtctcgag
     8221 cgtccggccg aagccgggcg gcacgacgag gaccagcccg cgcgagccgc ggcgcacgct
     8281 gtcgcgcgcc gcgccttcgt cggcgggcgc gggcgtcgcg cggaagcccc cgccggcgag
     8341 gctctgcgcg aggcgctcgg cgagcacggc gtcctccgtg ccgagcacgg cgaagtcggt
     8401 gctgcggccc gcgtcgtcct tgaaggcgtc ggacagcgcg agcgacatga tgacgatgaa
     8461 cgccgccggc atcaggaaca gcacggcgag gccgtgcacg tcccgcgcga gcgcgagcca
     8521 ttccttcttc caaagagcga gcagccgtcg catggttcgc gccttcagtc ccgcagcgag
     8581 cgccgcgtga gctgcatgaa cacctgctcg aggtcgcgct cgccgtaggc gaccgcgagc
     8641 ggctcgcagc ccgcggcgcg cgccgactcg atcgtccccg cgatcgcgga ggcgtcgggc
     8701 agccgcagcc gatagcgcgc gtcgccgtcg tcggcgagcg cgtagcgcgt ggtccattcc
     8761 gtggccagcc acttcggcag cggcgcgcgc aggcgcagcg tgagcaggtt cgacgcgtcg
     8821 cgcagcacgt cgtcgagcgt gcccgagagc agcacgcggc cgcggtcgat gatcgcgatg
     8881 cggtcgcaga tcgcctgcac ctcctccatg tagtggctgg tgtagacgat cacgcgcccg
     8941 ggccccgcga gcgagcggat cgagtcgagc aggaaggcgc gcgactgcgg atcgacgccg
     9001 accgtcggct cgtcgagcag cacgatgtcg ggctcggtga gcaggccgat cgcgaggttg
     9061 aggcggcgct tgaggcctcc cgagagctgt ccggcgcggc ggccgagcac gtcctcgatg
     9121 cgggcgaagg ccgcgaccgc ctcgacgcgc tcgcgcagcc gcgcgcccgc gagcccctgc
     9181 gctcccgcga agaagcgcag gttctcggcg cacgtgagca tgggatagaa ggcgtgctcc
     9241 tgcgggacca gccccaggca atgcgggcgc cgccggcgca gcgcgtcgag cggctcgccg
     9301 gcgaacgcga cgccgcccgc gccggcgcgc agctgtcccg agacgatcga gatcagcgtg
     9361 gtctttccgg ccccgttcgg cccgagcagg ccgaagatgc cgccggcggg gatctccagc
     9421 gcgacatcct ccagcacatc ggcgtcgccc cgcggatacc ggaacgacag gccctcgatg
     9481 ctcagcatcg ccacgcctgc tgcgcgcggc gcaggtcgcg gaagaacgac tcctcgtggt
     9541 ccgcgatccc gcgcagctgc gccgcgagcg cgggcggggc gagcggatcg cggcggcgga
     9601 tctggcgcgc ggccgcgacc gcgaattccc gccagcggtc gccgatgtcg accaggcgcg
     9661 aggcgaagcc ctcgagcgcg ccgtggccgg cgagcgcggc gagctcctgg aggaacgagg
     9721 cgtacaggaa gcggaacccg gcgccgccgg tgccgacctc ctcctgcatc ctgacgatgt
     9781 tgcccatgta catgcgcgcc gcgccgccgt tgccgtcgac cgggagacgc tcgacgcgct
     9841 tcgcgagcat gcggatgccg cgcacgccga tgatcggcag cggcgaatgc agcatgacgt
     9901 ggcaggtctt cgcgatcgcg cggcggccga cccgcgccca gtccggcgcc accggcttcc
     9961 gcgtcgggta gtagagcagg cccttcggcg cgagcatgcc tttcgcgaag cgcgccttct
    10021 gcaggtcggc gcgcgcgcag cgcaccagca tctcggcgac cgggtcgctg atcacgtact
    10081 cgtcgccctc gcgcgcgcac acgagcacgt tgtgcgcgtt gaaatggaag cgcaggtcgg
    10141 acggaaagta gggcagccag aacaccgagg tctgcaggcc gaccagcttg ccttcctcga
    10201 gcagctcgtc gaggcgcgcc atgccgcgct cgggcgagcg gaacgtctcg aagcgcatgc
    10261 ccaggccgag tcccttctcc agcccctgga tgatcgattt cggccacatg cggaacgcga
    10321 tcagcggcag gccgttgatg cgcacgaacg gcaggtatgc gaagcacagc gccgacgaca
    10381 gcccgaaggc catcgcctcg gtgaagggaa agccgtagtg gcgcacgagc gaggccatca
    10441 cgcccgactc gcagtgcgcg gcgtgcgcgt gcgtgaagct gccgtgctgc tttgtcgtcg
    10501 cgtcctgtgg ctgcatcgcc cgctgctgca tcgctagtcc agcttctcca gcgcctcgac
    10561 cgggattccc atcgcctcgg cgtagcgccg cttgatcgcg cccggcaggc gctcgaacgc
    10621 gcccgggcgc aggtggcgct tcacgcgcca gcgccacagg ccggtcgtct gcgacaggag
    10681 cggcacgtcc atgcgcgcgc ggtacatgtg gtactcgagc gtcgagccgg tgccgtcgag
    10741 cgcgcgctcg cgcgcctcgc gcgcaagccg ctcgagctcc tcgaccgcct ggcgcgtcac
    10801 cgtctcctcg acctcccagc cgcgcgaggc gacgatcttc agccggccgt cctcgcccac
    10861 cgcgtacacc gccttgcggt gcccggcgag cgtgacgttg ccctcctgcg ggacgtcgcg
    10921 ctcacgcatg attgacgacg gtgaggacca tgaagctgct cgagaagcgc ccgctctcgg
    10981 gcacgaagca cagcagccgg tcgccttcct tgaggcggcc gctcttcgcc agctcgtcga
    11041 gcatgaggta gaccgaggcc gagccggtgt tgccgacgtg gtcgaggttg gtgaaccact
    11101 tgtcctgcgg cagcggcagg cccgcgcgcg cgaagccgtc ggccaccggc tgacggaagt
    11161 actccgacga gatgtgcggc agcagccagg tgacgccggc cggcgtgacc tcgcgccgct
    11221 cgaggacctt gcgcagcggc ttcacgaccg tatactcgat caccgactcg ttcaggagcc
    11281 gcacgtcctg cttgatcgcg aacaccgagt ggcggtcgcg atcggcgacg tcgtagcgca
    11341 tccagccgcg caggcggccg tcggcctcct tctccgcgcc catgtacatg cacgcgggca
    11401 tcgcgtgtgc gtacgagaac acgtcgatcc actcgatgcg caggttgaga cccttgcgcg
    11461 gcgcgggctc gatcagcacc gcgcccgcgc cgtcggacag catccagcgc aggaagtcct
    11521 tctcgaacgc gaggccgggc tgcgcctcga gcgccgcgac gtcctgctgg cgctcgccgt
    11581 cgtagttgtg cgcgcgcagg ccgagcgacg cgacctccga gccggtcgcg atcgcgttct
    11641 tcgcgtcgcc cgacgcgacc gccatccagg cgtacttgag cgcggtcatg cccgagacgc
    11701 acacgcccga ggtcgcgacc acctcgcaca ccggcgcgcc gagctcgccg tgcaccatca
    11761 ccgcgtggtt gggcagcagc tggtccgggt tcgacgtgcc gcacgcgagg caggcgatgt
    11821 cctcgagcgc gaagccgccg ccgaccagtc ctttcaccgc cgcggcggtg aggctcgcgt
    11881 tgctgtgggt cggctcgccg gtcttggggt cgatcacgta gtggcgccgc cggatgccgt
    11941 tcgagcgctg caccaggcgc cgcgcgcgcg acggccggtc gcccacctgg ccgaggatcg
    12001 cctcgaccgc gtcgttctcg accggcgcgt tcggcagcag cgccgcggtg cgcgtgatgt
    12061 aggtcgtacg tcggctcatc gccgtctagc cgcccgaagg cagctcgaag tacgcctttt
    12121 gcgacgcgag gcgccgcgcg agcagcggac gcagcaaccg ctgcacgagc aggctgggcg
    12181 gcacgatgac gatcaccatc gccacgaggt agacgcagaa cagcgcgagg aacggcaggc
    12241 gggtcgccgc gccgcgcctg ccgccgaggc ggatcacgcg cgaccatgcg ccgaacgcgc
    12301 ggcgcgccgc gcgctcgctg aagatcaggg tcggatcgac tcgcgccgcg ccgagacccg
    12361 cgagcatcgg ccgtccgtcg cgctcgcgtc cggcgcgcag cgccgcgagg agcgcgcggc
    12421 cgaagcggtc ggcgcccgcg atctcctcct cggcgacgcc cgcgggcggc aggccgcaga
    12481 atgcgttttt ctttcccgtc agcacccagc gcggcgtcgt caccagggtc gcgaccgttc
    12541 cgccctggtc ggtgaacacg acgttgtctt tcaacacgcc gcccgcatcg gcgatcaggc
    12601 ccttcacggc ctcctgggcc accagccaca tgttgcggca ggcgatgacg gtcacgaccg
    12661 ggcgaccggc gagcagccgc ctgcccgtct cgctcttgag gaacgcggtg atcggaccgg
    12721 cgggcgcgag gaaccacacc tggtacgcga gaaccaccag atcgaaatgc tcgtcgccgc
    12781 gcacggcgag cggctcgagc gcgggcgggt cgagcagaac cgactcgggc atcgcgtcga
    12841 ggaagccgaa gaacgaccac gggaacgcgt acggcgcgac cggacgcagc gcctcttcga
    12901 cgagctcgac gtcgtccgcc gcggcgagcg gctcgacgag ccggcgcgcc acgcgcgtga
    12961 gctgccccga ttgcgagaaa tgaacgagaa ggacgcgctt cacggggcgg tcctcccggc
    13021 gatctgattc atttgcaacg tatttttgtc cggcgcccat tcgcccgtgg cgattcccga
    13081 gggcaccggt ccttccctgg ttaactaacc gcgaaggata ccccggcaga gggccggggt
    13141 gtcaggattt tgtcttcttt tgggcgcgcg atgccgcgga aatcaggcgg ccggcgctgc
    13201 cgggggctgt ccggcgccgc ctccgcgatt ccggcgacgg cggcggcgcc gccggccggg
    13261 ctcgccgggc cccggcgcga gcagcatcgt agtccgttcg gattcgccgg cgtgctggaa
    13321 tcgggtccac cactgcacca gctcctcggg gacctcgccg ctcgacgcgc gcagcacgag
    13381 gaagtcgtag gccgcgcgga agcgctcgtg ggcggtgagc gcgaacggac gccggccgct
    13441 tctctgctcg aagcgcggct gcatcgacca gatctccttc atgatcgcgg tgaagcggcg
    13501 cgggatcgag agcttcgaca cctggatgtc cacgaccgcg tccatcgcct ggtagagcgc
    13561 ggggatgcgc ttggcgcctt tcttctccgc cttgctccat gcggcgagca cctcgtgcca
    13621 cagcagcgcg gcgaacaaga acgcgggcga cacgggcttg cccgcgcgca cgcgctcgtc
    13681 ggtctgctcc agcgcgagct ggacgaagcg ctggccgagc ggctgctcga cgatcacgtc
    13741 gagcaacggc agcaggccgt ggtgcaggcc ttcgtcgcgc agctgccgca cgctggtcgc
    13801 ggcgtgcccg gagagcagca gcttcagcat ctcctcgaac acgcgcgccg gcggaacgtt
    13861 ctcgagcagc ggcgcgagct cgcggatcgg cgcgcgcgtc ttcgcatcga tcttgaagcc
    13921 gagcttcgcg gcgaagcgcg ccgcgcgcag catgcgtacc gggtcctcgc ggtagcgccc
    13981 gcgcgggtcg ccgatcatgc gcaccgactt gcgctcgagg tcgcgcaggc cgccgtggaa
    14041 gtcgaggatc gcctcgctgt agggatcgta gtagagcgcg ttcaccgtga ggtcgcgccg
    14101 cgcggcgtcg tcctcgcgcg tgccgtaaac gttgtcgcgc aggatgcggc cgtgcgcgtc
    14161 gacctggtga tccggcgcgg cgtcgcgctc gtgggtctcg ccggcgagcg tctcctcggg
    14221 ctcgggcgcc gagggcgcgc ggaacgtcga cacctcgacc gtctcgtcgc cgaacatcac
    14281 gtgcaccagg cggaagcgcc gtccgatcac gatcgcgcgg cggaacaggc gcttgatctc
    14341 ctcgggcctc gcgtcggggg cgacgtcgaa gtccttgggc tcgaggccga gcagcaggtc
    14401 gcgcacggcg ccgccgacca cgtaggcctg atagccgcgc tcgcgcagca cctcgcacac
    14461 cttgcgcgag ccggacgaga tgcgctcgcg cccgagcccg tgcttctcgc gcgggacgac
    14521 gacgggctga gggccggagc ccaggcggaa gacgcggctg atgaatcgct tgatcaagtg
    14581 gggatcgaca ggcccgcgga ctccaggcgg catcggtaca gcgcctcgag gcgggacggc
    14641 gtcagcatgt cggcgcagga tccggcgctg gagcggcccg catcatacaa caaaaccgcg
    14701 tgatcgcagt accgcgcggc gaggctcggc tggtgcagcg ccatgagcac cgcgcggccg
    14761 gcgcgggcgg actcggcgag gagctccatg acctgcacct ggtggcgcag gtcgagatgg
    14821 ctgagcggct cgtcgagcag cagcagggcc gcgtcctgca cgagcagctg cgcgatgcgc
    14881 gcgcgctggc gctcgccgcc cgagagcgtg acgtagggct gacgcgcgcg ctcggcgagg
    14941 tcgagccgcg cgagccagcc gagcgcggcg tcgtcgtcct cggcgccgcc tccccacgcg
    15001 ccgcccgagc ggggatagcg cccgagccgc gcgtactcga gcgcggtgcc ccagaagtcc
    15061 ggcgtgtcgt cctgcagcag gacaccgacg cggcgcgcga gctcgctccg gcgccagcgc
    15121 gcgagcggac gctcgtcgag cgcgatctcg ccccggtgcg gcgcgcgcag gcccgcgagc
    15181 gccaggatca ggctcgattt tccagccccg ttgggcccga gcagcgccca gcactggccg
    15241 ggcgcgagcg cgaggtccag tccttcgacc agcgttcgtc cgccagcctg gatgccgatc
    15301 gcgcgcgcgg cgagcatggc tagcgcacgc gcgcgagcag gaacagcagc gccggaacgc
    15361 cgatcagcgc cgtgatcacg ccgaccggca gctccgaggg cgcggtcgcg gtgcgcgcca
    15421 cggtatccgc caccacgagc aggctcccgc ccgcgcacac cgagagcggc agcagcaggc
    15481 ggtgggcccg gactcccgcg agacgcagca cgtgcgggac gacgaggccg acgaagccga
    15541 tcgcgccgcc gaggagcacc gccgcgacgg tggcgagcgt cgcggccgcg aacgcgatca
    15601 cctgcagccg cgcgacatcg accccgagcg aggcggcctt gtcgctcccg aggagcacga
    15661 cgtcgagcgc ggacgcgcg
//
LOCUS       DATDMW010000147         8663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2160952, whole genome shotgun sequence.
ACCESSION   DATDMW010000147 DATDMW010000000
VERSION     DATDMW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160952"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VI319_06540"
     CDS             <1..235
                     /locus_tag="VI319_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863542.1"
                     /translation="GLSAIVHADRNGANSAAPVRVDGPVLQTAAGAELEAFRAEKRER
                     LESYGWSDRRAGRAHIPIERAMALEAKEAEKTR"
     gene            232..966
                     /locus_tag="VI319_06545"
     CDS             232..966
                     /locus_tag="VI319_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY6863543.1"
                     /translation="MRRLFHVILSAAKDLLLLLAFVALPASAHLAHPERLAWDPHPGA
                     SVPIDLRFGDATAPVVLVLGYLRCPNLCDTTLAGVTEGLRMAGLRPGPDYRALFVSID
                     PLDTPALAAETKARRLPADARGAWRFVTGDTAAIARAAGFRYARDGDEYAHPVGFVVL
                     TPDGKVSRTFPGVRFDPDELRAALAEARRGERGPLARPLLLLCAHLDPAGAHTTAILD
                     ALRAAGVLFLAGAAMFAWRAGRKGSR"
     gene            963..1901
                     /gene="coxB"
                     /locus_tag="VI319_06550"
     CDS             963..1901
                     /gene="coxB"
                     /locus_tag="VI319_06550"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY6863544.1"
                     /translation="MSGGIFWLPPAASDVAARVDALFFAMVALTGTVALAITIVVLVF
                     AVRYRRGSRADRSNAPAKKLPVEIAWTVTPLAIFLGVFVWGAAVYAGLYRPAADAMPV
                     YVVGKQWMWKAEHANGRREIDELHLPAGQPVRLVLATQDVIHSFYVPAFRIKQDAVPG
                     RYTTIAFTPTKAGTFALRCAEYCGTDHAHMGGRVIVMQPADFQRWLAQGAPAGTMAAR
                     GFARFRELGCSGCHDPRSSVHAPDLAGLYGRTVHLADGRSVVADESYIRDSILLPKRD
                     VVAGFDPIMPSFQGQVGEDDLLELIAYIKSTKSESG"
     gene            1898..3511
                     /gene="ctaD"
                     /locus_tag="VI319_06555"
     CDS             1898..3511
                     /gene="ctaD"
                     /locus_tag="VI319_06555"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY6863545.1"
                     /translation="MSYLEEGYTLRSWLTTHDHKRIAILYAIAITAFFFVGGAAATMI
                     RLELATPQADLVQADTYNKLFTIHGVIMVWFFLIPSIPATLGNFLMPLMIGARDLAFP
                     RLNLASWYIYVLGGLFTVATLIAGGVDTGWTFYTPYSTMFSNTNVILAAAGVFITGFS
                     SILTGLNFIVTVHKMRAPGMTWFRLPLFVWAHYATSVILVLATPVLAMTLVLIAAERL
                     LHVGVFDPALGGDPLLFQHLFWFYSHPAVYIMVLPAMGVASEIITCFARKRVFGYRFM
                     AYAILGIATIGFLVWGHHMFVSGQSMYAGMVFSVLSFMVAVPSAIKVFNWTATLYKGS
                     ITFSAPMLYALGFVGLFMIGGMTGLILATLALDVHLTDTYFVVAHFHYIMVGGSVMAY
                     LGGIHYWWPKATGRMYPEMWGRVAAIVIFVGFNLTFFPQYLLGYYGMPRRYHVYPPEF
                     TVLNVLSSAGASILAIGYLLPIGYLAWSLFRGKKAGANPWDARGLEWTTPSPPPKDNF
                     AVAPTVTEEPYAYSPERDAEMAGGRIAQP"
     gene            3508..4149
                     /locus_tag="VI319_06560"
     CDS             3508..4149
                     /locus_tag="VI319_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY6863546.1"
                     /translation="MSVAHRIAEQFETREQQREADSLGTWVFLATELMFFGPLFLAYF
                     YGRTHYGEGFAVASGRTHVWLGTANTALLLTSSLTMALAVRASWIGRRGVTLLLALTA
                     ALGVAFLAVKGIEYRKEWDEALVPPFRFVFAGEHARAVEVFYWLYFLITGLHALHLTI
                     GIGLVLWLALRARRGDFGPHYHAPVEMTGLYWHFVDVLWIFLYPLIYLLERWR"
     gene            4146..4463
                     /locus_tag="VI319_06565"
     CDS             4146..4463
                     /locus_tag="VI319_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY6863547.1"
                     /translation="MNPAPVRTYVLVWAALIALLALTAASAFVNLGAFNSVANLAIAA
                     AKALLVAAFFMHLRRHASALVRVFAGAALFMLALLFALSGADYATRHIAPAAWSSPPS
                     PTP"
     gene            4472..5491
                     /locus_tag="VI319_06570"
     CDS             4472..5491
                     /locus_tag="VI319_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6863548.1"
                     /translation="MQQAPIVVITGASAGVGRAAARHFGSRGWRVALLARGADGLEAA
                     RREIEAAGGRAMAIPADVADHAQVEAAAERVEREWGPIDVWVNDAMATIFCELEKIAP
                     EDFRRSTEVTYLGTVWGTMAALKRMSARNRGAIVQVGSALAYRSIPLQAPYCGAKSAI
                     RGFTDSLRSELIHKRSKVHVTMVQLSAFNTPQFDWGRTCMTRQPQPLPPIFQPEVAAE
                     AIYYAATHRRRELWVGWPAIKAIVGARFLPALGDRILATEAYRGQHTDEPLPAGRRDN
                     LYAPVPGDHGAHGRFDAHARRTSVQLWLNLRPWIGLAAGVGIALAAAALRLRNIGPGR
                     RVSRL"
     gene            5503..5811
                     /locus_tag="VI319_06575"
     CDS             5503..5811
                     /locus_tag="VI319_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863549.1"
                     /translation="MLTALALAVPMTAWAKLPPPTPAEQDRLEQQKAAKAAATEQDKK
                     ELAQVQDRIVKRYRTRHPDYAAAHPAVRTNAPLTDADVPKAARVPGPSGQPHSGRNES
                     "
     gene            complement(5808..8471)
                     /locus_tag="VI319_06580"
     CDS             complement(5808..8471)
                     /locus_tag="VI319_06580"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY6863550.1"
                     /translation="MATPLARALIACLALLASAAPAATPPRAEDVARATLANGMRVVV
                     VRNALAPVVSTSLNYLVGANETPEGFPGTAHAVEHIMFRGSPGVSADQLADIGSIMGG
                     EFNADTRQTVTQYLYTVPAEDLGVVLNIEALRMRSVLAGEDDWKGERGAIEQEVAQDL
                     SNPQYNLYTRLRAAVFAGSPYAHDGLGTRESFEKTSGAMLKDFHDRWYAPNNAILIVV
                     GNVDPSATLAQVRKLFEPIASRPLPARPEVAFAPVAPQSFTLESDLAYGLQVIAMRFP
                     GLEDADFPAVEVLIDVLKSRRGELYGLVPEGAALATDFSFEPLPKAGLAYALAAFPAG
                     GDAKALETRMRAILAKVAQEGVPADLVAAAKLQERRDAELQRNSIAGLAAVWSEAVAV
                     YGLESPDEDLARIERVTVEDVNRVARKVLDLDHAVTAVMTPVGSGKPVASQGFGGKEK
                     LAPGEIKPVPLPDWAAPLARLSVPASSVRPVVSRLPNGITLIVQPEDVSASVSVYGHV
                     ENRPEMQVPKGREGLAEVMEQLFPYGSRRLDRVALQRALDEIGADASAGTDFSLNVLA
                     EHFDRGVGLLADNLLEPGFPAAAFEVVKRQVADTVAGQLSTPSYLGRMALRAGLFPKN
                     DPSLREPSPKTVGRVTLADVRAYYRAVFRPDLTTIVVIGRITPERARAVVQRYFGGWT
                     AVGPAPRTTLPPVPLNRAAVSAVPDASRVQDRVILGETLGLTRAHPDSYALDLGNAVL
                     GGAFYSTRLTRDLRKEAGLVYYVGSSLQYGRTRTIYLVDYACDPQNVGKVHDIVAGEL
                     VQMQKTPVGEDELGRAKALLVRRLPLEEASVDGIARGFIDRRTLNLPLDEPARAARRY
                     AALGARDVQAAFAKWVRPGALVRVSEGPAPR"
     gene            8470..>8663
                     /locus_tag="VI319_06585"
     CDS             8470..>8663
                     /locus_tag="VI319_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863551.1"
                     /translation="MVLQTSTQARGSPASAWRRAAAPRRGTSRSLAGPICMSRPFAAA
                     AAVLGALLVYAALGTLVAPRL"
BASE COUNT         1143 a         3196 c         3010 g         1314 t
ORIGIN      
        1 gggcctgtcc gcgatcgtgc atgccgaccg caacggcgcg aacagcgcgg cgccggtgcg
       61 cgtcgacggc ccggtcctgc agaccgcggc cggcgccgaa ctcgaggcct tccgcgccga
      121 gaagcgcgag cgcctcgaga gctacggctg gagcgatcgc cgcgcgggcc gcgcgcacat
      181 cccgatcgag cgcgcgatgg cgctcgaggc gaaggaagcg gagaagaccc ggtgaggcgc
      241 ctgttccatg tcatcctgag cgcagcgaag gatctgcttt tgttgctcgc cttcgtcgcg
      301 ctgccggcct cggcgcatct cgcccatccc gagcggctcg cctgggaccc gcacccgggc
      361 gcgagcgtgc cgatcgacct gcgcttcggc gacgcgacgg cgccggtggt gctcgtgctc
      421 ggctacctgc gctgcccgaa tctctgcgac accacgctcg ccggcgtgac cgagggcctg
      481 cgcatggcgg ggctgcgccc gggcccggac tatcgcgcgc tcttcgtcag catcgatccg
      541 ctcgacacgc ccgcgctcgc agccgagacg aaggcgaggc gcctgccggc cgatgcccgc
      601 ggcgcctggc gcttcgtcac gggcgacacg gccgccatcg cgcgggccgc gggcttccgc
      661 tacgcgcgcg acggggacga atacgcgcat cccgtgggct tcgtggtgct gacgccggac
      721 ggcaaagtgt cccgcacgtt tcccggcgtg cgcttcgatc cggacgagct gcgcgccgcg
      781 ctcgccgagg cgcgccgcgg cgagcgcggt cccctcgcgc ggcccctgct gctgctctgc
      841 gcgcacctcg accccgcggg cgcgcacacc accgcgatcc tcgacgcgct gcgcgccgcc
      901 ggcgtcctgt tcctcgccgg cgcggcgatg ttcgcgtggc gcgccggccg gaaggggtcg
      961 cggtgagcgg agggatcttc tggctgccgc ccgcggcctc ggacgtcgcc gcgcgcgtcg
     1021 acgcgctgtt cttcgcgatg gtcgcgctga ccggcacggt ggcgctcgcg atcaccatcg
     1081 tcgtgctggt gttcgcggtg cgctaccgcc gcggctcgcg cgccgaccgc tcgaacgcgc
     1141 cggcgaagaa gctgccggtc gagatcgcgt ggaccgtgac gccgctcgcg atcttcctcg
     1201 gcgtgttcgt ctggggcgcc gcggtgtacg cgggcctcta ccggcccgcg gccgacgcga
     1261 tgcccgtcta cgtcgtcggc aagcagtgga tgtggaaggc cgagcacgcg aacggccggc
     1321 gcgagatcga cgagctgcac ctgccggccg gccagccggt gcggctcgtg ctcgcgacgc
     1381 aggacgtgat ccacagcttc tacgtcccgg cgttccgcat caagcaggac gccgtgcccg
     1441 ggcgctacac cacgatcgcg ttcacgccga cgaaggcggg gacgttcgcg ctgcgctgcg
     1501 ccgaatactg cggcaccgac cacgcgcaca tgggcgggcg cgtgatcgtg atgcagcccg
     1561 ccgatttcca gcgctggctc gcgcagggcg cgccggcggg caccatggcg gcgcgcggct
     1621 tcgcgcgctt ccgcgagctg ggctgcagcg gctgccacga cccgcgctcg agcgtgcacg
     1681 cgcccgacct cgcgggcctg tacgggcgca cggtccacct cgccgacggg cgcagcgtcg
     1741 tcgccgacga gtcctacatc cgcgactcga tcctgctgcc gaagcgcgac gtcgtcgcgg
     1801 gcttcgatcc gatcatgccg tcgttccagg gacaggtggg cgaggacgac ctgctcgagc
     1861 tgatcgcgta catcaagtcg acgaagagcg agagcggatg agctacctcg aggaaggcta
     1921 cacgctgcgc tcgtggctga cgacgcacga ccacaagcgc atcgcgatcc tgtacgcgat
     1981 cgcgatcacc gccttcttct tcgtcggcgg cgcggcggcg acgatgatcc ggctggagct
     2041 cgcgacgccg caggccgacc tggtgcaggc cgacacctac aacaagctgt tcaccatcca
     2101 cggcgtgatc atggtgtggt tcttcctgat cccgtcgatc ccggcgacgc tcggaaactt
     2161 cctcatgccg ctgatgatcg gcgcgcgcga cctcgccttc ccgcggctca acctcgcgtc
     2221 ctggtacatc tacgtgctcg ggggcctgtt cacggtcgcg accctgatcg cgggcggcgt
     2281 cgacaccgga tggaccttct acacgccgta ctcgacgatg ttctcgaaca ccaacgtgat
     2341 cctcgccgcg gcgggcgtgt tcatcaccgg gttctcctcg atcctcacgg gcctgaactt
     2401 catcgtcacc gtgcacaaga tgcgcgcgcc ggggatgacc tggttccggc tgccgctgtt
     2461 cgtctgggcg cactacgcga ccagcgtcat cctcgtgctc gcgaccccgg tgctggcgat
     2521 gacgctggtg ctgatcgcgg ccgagcggct gctgcacgtc ggcgtgttcg atcccgcgct
     2581 cggcggcgat ccgctgctct tccagcacct gttctggttc tactcgcacc cggcggtcta
     2641 catcatggtg cttccggcga tgggcgtggc gagcgagatc atcacctgct tcgcgcgcaa
     2701 gcgcgtcttc ggctaccgct tcatggccta cgccatcctc ggcatcgcca cgatcggctt
     2761 cctcgtctgg ggccaccaca tgttcgtctc gggacagtcc atgtacgcgg gcatggtgtt
     2821 ctcggtgctc tcgttcatgg tcgccgtgcc ctcggcgatc aaggtgttca actggaccgc
     2881 gaccctgtac aagggctcga tcacgttctc cgcgccgatg ctgtacgcgc tcggcttcgt
     2941 cggcctgttc atgatcggcg gcatgaccgg actgatcctc gcgacgctcg cgctcgacgt
     3001 gcacctcacc gacacctact tcgtggtcgc gcacttccac tacatcatgg tcggcggctc
     3061 ggtgatggcc tacctcggcg gcatccacta ctggtggccg aaggcgaccg ggcgcatgta
     3121 cccggagatg tgggggcgcg tcgccgcgat cgtcatcttc gtcggcttca acctcacctt
     3181 cttcccgcag tacctgctcg gctactacgg catgccgcgg cgctaccacg tctacccgcc
     3241 cgagttcacg gtcctgaacg tgctgtcctc ggccggcgcg tcgatcctcg cgatcggcta
     3301 cctgctcccg atcggctatc tcgcgtggtc gctcttccgg gggaagaagg cgggcgcgaa
     3361 cccctgggac gcccgcggcc tggagtggac cacgccctcg ccgccgccca aggacaactt
     3421 cgccgtcgcg ccgaccgtga ccgaggagcc gtacgcctac tcgcccgagc gcgacgcgga
     3481 gatggcgggc gggaggatcg cccagccatg agcgtcgccc accgcatcgc cgagcagttc
     3541 gagacacgcg agcagcagcg cgaggccgac tcgctcggca cctgggtgtt cctcgcgacc
     3601 gagctgatgt tcttcgggcc gctcttcctc gcctacttct acggtcgaac ccattacggc
     3661 gagggcttcg cggtcgcgag cggccgcacg cacgtgtggc tcgggacggc gaacaccgcg
     3721 ctgctgctca cgagcagcct caccatggcg ctcgcggtgc gcgcgtcgtg gatcggccgg
     3781 cgcggcgtga cgctgctgct cgccctcacc gccgcgctcg gcgtcgcctt cctcgccgtc
     3841 aaaggcatcg agtaccgcaa ggagtgggac gaggcgctgg tgccgccgtt ccgcttcgtg
     3901 ttcgcgggcg agcacgcgcg cgcggtcgag gtgttctact ggctgtactt cctcatcacc
     3961 gggctgcacg cgctgcacct gacgatcggc atcggactcg tgctgtggct cgcgctccgc
     4021 gcgcggcgcg gcgacttcgg gccgcattac cacgccccgg tcgagatgac cgggctgtac
     4081 tggcatttcg tcgacgtcct ctggatcttc ctttaccccc tcatctacct gctggagcgc
     4141 tggcgatgaa tccggctccg gtgcgcactt acgttctcgt atgggccgcg ctgatcgcgc
     4201 tgctcgcgct gaccgcggcg agcgccttcg tgaacctcgg ggccttcaat tcggtcgcga
     4261 acctcgcgat cgcggcggcc aaggcgctcc tggtcgcggc cttcttcatg cacctgcgcc
     4321 gccacgcgtc cgccctggtg cgcgtgttcg cgggcgcggc gctgttcatg ctggcgctgc
     4381 tgttcgcgct gagcggcgcc gattacgcca cccgtcatat cgcgccggcc gcgtggtcgt
     4441 cgccgccgtc gccgacaccg taggaggctc catgcagcaa gcacccatcg tcgtcatcac
     4501 cggcgcctcg gccggggtcg ggcgcgccgc cgcgcgtcat ttcggctcgc gcggctggcg
     4561 cgtcgccctg ctcgcccgcg gcgcggacgg cctcgaggcc gcgcgccgcg agatcgaggc
     4621 cgcgggcggc cgcgcgatgg cgatcccggc cgacgtcgcc gatcacgccc aggtcgaggc
     4681 cgcggccgag cgcgtcgagc gcgagtgggg cccgatcgac gtctgggtga acgatgcgat
     4741 ggcgaccatc ttctgcgagc tcgagaagat cgcgccggag gacttccgcc gctcgaccga
     4801 ggtgacgtat ctcggcaccg tgtggggaac gatggccgcg ctcaagcgca tgagcgcgag
     4861 gaaccgcggc gcgatcgtgc aagtgggctc ggcgctcgcg taccgctcga tcccgctcca
     4921 ggccccttat tgcggcgcga agagcgcgat ccgcggcttc accgactcgc tgcgatccga
     4981 gctgatccac aagcgcagca aggtgcacgt cacgatggtg cagctctcgg cgttcaacac
     5041 gccgcagttc gactggggcc gcacctgcat gacgaggcag ccgcagccgc tgccgccgat
     5101 cttccagccc gaggtcgcgg ccgaggcgat ctactacgcc gcgacgcacc gccggcgcga
     5161 gctctgggtc ggctggcccg cgatcaaggc catcgtcggc gcccggttcc tgcccgcgct
     5221 cggcgaccgc atcctcgcga ccgaggcgta ccgcggccag cacaccgacg agccgctgcc
     5281 ggcggggcgc cgcgacaacc tctacgcgcc cgtgcccggc gaccacggcg cgcacgggcg
     5341 cttcgatgcg catgcgcgcc ggacgagcgt ccagctgtgg ctcaacctgc ggccgtggat
     5401 cgggctggcg gcgggcgtcg ggatcgcgct cgccgctgcg gcgctcaggc tgcggaacat
     5461 cggcccgggt cgacgcgtct cacgactatg aggatctttc acgtgttgac ggcgctcgcc
     5521 ctcgcggtcc cgatgacggc atgggcgaag ctgccgccgc ccacccccgc cgagcaagac
     5581 aggctcgagc agcagaaggc ggccaaggcc gcggccaccg agcaggacaa gaaagagctc
     5641 gcgcaggtgc aggaccggat cgtgaagcgc taccgcacgc gccacccgga ctacgccgcc
     5701 gcgcatcccg cggtccggac gaacgccccg ctcaccgacg ccgacgtgcc caaggcggcg
     5761 cgcgtaccgg ggccttcggg ccagccgcat tccgggcgca acgagtccta gcgcggcgcc
     5821 gggccctcgc tgacgcgcac cagcgcgccg ggccggaccc acttcgcgaa cgccgcctgc
     5881 acgtcgcgcg cgccgagcgc cgcgtagcgc cgcgccgcgc gcgccggctc gtcgagcggc
     5941 aggttcaggg tgcggcgatc gatgaagccg cgcgcgatgc cgtccacgct cgcctcctcg
     6001 agcggcagcc ggcgcacgag cagcgccttc gcgcgtccga gctcgtcctc gccgaccggc
     6061 gtcttctgca tctgcaccag ctcgcctgcg acgatgtcgt gcaccttgcc gacgttctgc
     6121 gggtcgcagg cgtagtcgac gaggtagatc gtccgcgtgc gcccatactg cagcgacgag
     6181 cccacgtagt agaccagccc cgcctccttg cgcaggtcgc gcgtgagccg cgtcgagtag
     6241 aacgcgccgc cgagcaccgc gttgccgagg tcgagcgcgt acgagtcggg gtgcgcgcgc
     6301 gtgagcccga gcgtttcgcc gaggatcacg cggtcctgca cgcggctcgc atcgggcacg
     6361 gcgctcaccg ccgcgcggtt gagcgggacc ggcggcagcg tggtccgcgg cgccgggccg
     6421 acggcggtcc agcccccgaa ataccgctgc acgacggcgc gcgcgcgctc gggcgtgatc
     6481 ctgccgatca ccacgatggt ggtgagatcg ggacggaaca cggcgcggta gtaggcgcgc
     6541 acgtcggcga gcgtgacccg gcccaccgtc ttcggcgaag gctcgcgcag gctcggatcg
     6601 ttcttcggga acagccccgc gcgcagcgcc atgcgcccga ggtacgacgg ggtcgagagc
     6661 tggccggcga cggtgtcggc gacctggcgc ttcacgacct cgaacgccgc ggcggggaat
     6721 cccggctcga gcaggttgtc ggcgagcagg ccgacgccgc ggtcgaagtg ctcggcgagc
     6781 acgttcagcg agaaatcggt gcctgcgctc gcgtccgcgc cgatctcgtc gagcgcgcgc
     6841 tgcagcgcga cgcggtccag gcgccgcgag ccgtacggga agagctgctc catcacctcg
     6901 gcgagtccct cgcggccctt cgggacctgc atctcgggcc ggttctcgac gtggccgtag
     6961 acgctcacgc tcgcgctcac gtcctcgggc tgcacgatga gcgtgatgcc gttcggcagc
     7021 cggctcacga ccgggcgcac gctcgacgcc ggcaccgaca ggcgcgcgag cggcgcggcc
     7081 cagtccggca gcggcacggg cttgatctcg cccggcgcga gcttctcctt tccgccgaag
     7141 ccctgcgacg cgaccggctt gcccgacccg accggcgtca tcaccgcggt cacggcatgg
     7201 tcgaggtcga gcaccttgcg cgcgacgcgg ttcacgtcct cgaccgtcac gcgctcgatg
     7261 cgcgcgaggt cctcgtcggg cgattccagc ccgtagaccg cgaccgcctc cgaccacacc
     7321 gcggcgaggc ccgcgatcga gttgcgctgc agctcggcgt cgcgccgctc ctgcagcttc
     7381 gccgcggcga cgaggtcggc cggcacgccc tcctgcgcga ccttcgcgag gatcgcgcgc
     7441 atcctcgtct ccagcgcctt cgcgtcgccg cccgccggaa aggcggcgag cgcgtacgcg
     7501 agccccgcct tggggagcgg ctcgaacgag aagtcggtcg cgagcgccgc gccctcgggc
     7561 acgaggccgt agagctcgcc gcggcggctc ttcagcacgt cgatcagcac ctcgaccgcg
     7621 gggaagtcgg cgtcctcgag ccccgggaag cgcatcgcga tcacctgcag gccgtaggcg
     7681 agatcgctct cgagcgtgaa cgactgcggc gcgaccggcg cgaacgcgac ctcgggccgc
     7741 gccggcagcg ggcgggacgc gatcggctcg aagagcttgc gcacctgcgc cagcgtcgcc
     7801 gacggatcga cattgccgac gacgatcagg atggcgttgt tcggggcgta ccagcggtcg
     7861 tggaagtcct tgagcatcgc gccgctcgtc ttctcgaacg actcgcgcgt gccgaggccg
     7921 tcgtgcgcgt agggcgagcc cgcgaacacc gcagcgcgca ggcgcgtata gaggttgtac
     7981 tgcgggttcg acaggtcctg cgcgacctcc tgctcgatcg cgccgcgctc gcccttccag
     8041 tcgtcctcgc cggcgagcac cgagcgcatg cgcagcgcct cgatgttcag caccacgccg
     8101 agatcctcgg ccggcaccgt atacaggtac tgcgtgacgg tctggcgcgt gtcggcgttg
     8161 aactcgccgc ccatgatgct gccgatgtcg gcgagctggt cggccgacac tcccgggctg
     8221 ccgcggaaca tgatgtgctc gaccgcgtgc gccgtccccg ggaagccctc cggcgtctcg
     8281 ttcgcgccga cgaggtagtt caggctggtc gagacgaccg gcgcgagcgc gttgcgcacg
     8341 acgacgacgc gcatgccgtt ggcgagcgtc gcgcgcgcga cgtcctcggc gcgcggcggc
     8401 gtcgcggccg gcgcggccga cgcgaggaga gcgaggcacg cgatcagcgc gcgggcgagg
     8461 ggcgtggcca tggttcttca gactagcacg caggcccgcg gttcgcccgc gtccgcatgg
     8521 cgcagggcgg ccgcgccccg ccgcggaacc agtcgatcgc tcgccggccc aatctgcatg
     8581 agcaggccct tcgcagcggc ggcggccgtg ctgggcgcgc tgctcgtcta tgccgcgctc
     8641 gggaccctgg tcgcgccgcg gct
//
LOCUS       DATDMW010000148        14894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_169655, whole genome shotgun sequence.
ACCESSION   DATDMW010000148 DATDMW010000000
VERSION     DATDMW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14894
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169655"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /gene="acnA"
                     /locus_tag="VI319_06590"
     CDS             complement(<1..1607)
                     /gene="acnA"
                     /locus_tag="VI319_06590"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY6863552.1"
                     /translation="MAHNLFNTLQEFKMASGKKGKLYSLPALEKALGAKVSRLPVSLR
                     IVLEAVLRHCDGKKVTEDHVRQLASWKAKAARTEEIPFVLARILLQDFTGIPALVDLA
                     AMRNVAEKVGKDPKVIEPLVPVDLVVDHSVQIDHYGAKNALDLNMRIEFERNKERYQF
                     MKWAMQAFDTFKVVPPGIGIVHQVNLEYLARGVHEKDGIYYPDSLVGTDSHTTMINGI
                     GVVAWGVGGIEAEAGMLGQPNYFLTPDVVGVHLKGRLRGGVTATDLVLTVTEMLRKAK
                     VVGKFVEFFGEGAESLTVPDRATLGNMAPEYGATMGFFPVDDQTIAYFEGTGRSKDEV
                     DALRSYFKAQGMYGVPKPGQIDYSEVVELDLATVAPSVAGPKRPQDRIEIGKVKGKFG
                     ELFSKPVAEAGFAKRPEDLAKRFKTRDGIELGSGDVLIAAITSCTNTSNPGVLLAAGL
                     LAKKAVERGLTVKKHVKTSLAPGSRVVTDYLTKTGLLPYLEKLGFYLSAYGCTTCIGN
                     SGPLAETIEETVNKSDLVCASVLSGNR"
     gene            complement(1627..3069)
                     /locus_tag="VI319_06595"
     CDS             complement(1627..3069)
                     /locus_tag="VI319_06595"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEY6863553.1"
                     /translation="MSAPISNVRPKPDKVLVDIADYVARHEIESAEAYETARLCLMDT
                     LGCGLEALEYPACTKLLGPLVPGTVIPNGARVPGTSYQLDPVNAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILAVADWLSRTAGADRREPLVVRDVLTAMIKAHEIQGVIAL
                     ENSFNRVGLDHVVLVKVASAAVVSKMLGLDRDRIIDAVSNAWIDGQSLRTYRHAPNTG
                     SRKSWAAGDATARAVRLALMVRAGEMGYPSALSAKTWGFYDVLFRGKPFKFTRKYGSY
                     VMENVLFKISFPAEFHAQTAVEAAIALHPKVRDRLADVKKIEIVTHESAIRIIDKKGP
                     LHNPADRDHCIQYMVAVGLLKGALTAADYEDAVARDPRIDALRAKMVCIENKRWSKDY
                     LDPKKRSIANQIQIFFRGGGATPKVAVEYPLGHRRRRREGIPALEAKFRANLARRFPE
                     ARQKQILDLCQDAKRLEATPVDQFVDLFVN"
     gene            complement(3072..3623)
                     /locus_tag="VI319_06600"
     CDS             complement(3072..3623)
                     /locus_tag="VI319_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863554.1"
                     /translation="MTRFKLACFGVLCATGTAVAAPADIAGTWECRQPGVNYGNKPPI
                     LYFTDAPGGGASAVTVEVDGFSREVYGRAELAQDPDGWLKVKPAKGQEFSVKPEADAR
                     QRTASMALRWPEAKAEYRCLRLPSSASRPVTESSPGAANPQGTLAVPPSGAAPQEAAP
                     QEAAPQNAAPPQAPTPQEPEKKQ"
     gene            complement(3643..4794)
                     /gene="prpC"
                     /locus_tag="VI319_06605"
     CDS             complement(3643..4794)
                     /gene="prpC"
                     /locus_tag="VI319_06605"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEY6863555.1"
                     /translation="MSDLTMAPKPKKSVALSGVPAGNTALCTVGRTGNDLHYRGYDIL
                     DFADQAEFEEVAHLLVHEKLPTAAELAGYKKKLKALRGLPVAVKAALEVVPASAHPMD
                     VMRTAVSVLGTTLPEKDDHNVPGARDIADRLMACLGSALLYWYHWSHNGRRIEVETDD
                     DSIGGHFLHLLHGKAPSAEWVRAMHTSLILYAEHEFNASTFTCRVVAGTGSDMYSAIC
                     GGIGALRGPKHGGANEVAFEVQSRYDTPDQAEADVVRRIGNKEIVIGFGHPVYTIADP
                     RNEVIKAVAKRLSKSAGDTKLYDIAERLEAVMAREKKMFANLDWYSAVSYHVMGVPTA
                     MFTPIFVISRTSGWSAHIIEQRIDGKIIRPGANYTGPEDQRWVPLKDRR"
     gene            complement(4791..5681)
                     /gene="prpB"
                     /locus_tag="VI319_06610"
     CDS             complement(4791..5681)
                     /gene="prpB"
                     /locus_tag="VI319_06610"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY6863556.1"
                     /translation="MSAASAGARLRAAMQAEKPLQCVGTINAYHARLAERVGYRSIYL
                     SGGGVAAGSLGLPDLGISNLEDVLIDVRRITDVCALPLLVDVDTGFGASAFNIARTVK
                     SLIKAGAAGMHIEDQVGAKRCGHRPGKELVAKEEMVDRVKAAVDAKTDPDFLVMARTD
                     ALAVEGLERAIERAVACVAAGADAIFPEAMTDLAMYRRFADAVKVPVLANITEFGSTP
                     LFTLDELRSANVAIALYPLSAFRAMNAAALGVYEAIRKEGTQKNVVGQMQTRADLYDY
                     LDYHSYEKKLDQLFAKGKDK"
     gene            complement(5678..6685)
                     /locus_tag="VI319_06615"
     CDS             complement(5678..6685)
                     /locus_tag="VI319_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY6863557.1"
                     /translation="MSKPLHPHAALFKGERPFPVIPSCEHFAGNENFIGKAFEIQHRL
                     GPIFDITCDCEDGAAPGREREHAEMIVRMLASPANRFRHAGVRIHDFSHPHWRRDVDI
                     LVEGAGELLAYITLPKATSAAQVADMIGYVQQVAAHHGIRREIPIHALIETHGGLREA
                     YEIAGLPWMQALAFGQMDFVSSHHGAIPASAMRSPGQFEHKLLARAKARVAAAALGNG
                     IVPTHNVTQDLKNAYVTYEDAWRARQEYGFMRMWSIHPTQIQPIVDAMKPDFSEVRDA
                     AGILLAAQRADWGPIQYAGELHDRASYRYYWEVLQKAKVTGVPIPEDAARAFFETERQ
                     P"
     gene            complement(6696..7688)
                     /locus_tag="VI319_06620"
     CDS             complement(6696..7688)
                     /locus_tag="VI319_06620"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY6863558.1"
                     /translation="MSKSPMRVAVTGAAGQIGYSLLFRIASGAMLGADQPVVLQLLEI
                     PDEKAQKALKGVMMELDDCAFPLLAGMAATSDPMVAFKDVDVALLVGARPRGPGMERK
                     DLLEANGKIFAPQGQALDRVAKRDVRVLVVGNPANTNCLIAMKNAKSLKPAQFTAMMR
                     LDHNRAVSQIAQKIGKPIASVRKVAVWGNHSSTQYPDVFHAEVDGKKVWPMINDQQWL
                     ESSFIPTVQKRGAAIIEARGLSSAASAANAAIDHVRDWVHGTREGDWVSMGIPSDGSY
                     GIPEGVLYGYPVTCRGGKYEIVKGLDISDFARKRMDATLKELQEERDGIKALLG"
     gene            7832..8221
                     /gene="sdhC"
                     /locus_tag="VI319_06625"
     CDS             7832..8221
                     /gene="sdhC"
                     /locus_tag="VI319_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY6863559.1"
                     /translation="MPDLAAKRKRPVWFNLSLLKLPAPGLVSIFHRASGAWLFLGLIW
                     FLFLLEMSLGSEAGYEHARAYVAHPVVKLALLAFLWSYLHHFCAGIRYLFLDMDVGLP
                     LAAARRSAFVVFAVSLALTVLIGARLW"
     gene            8218..8562
                     /gene="sdhD"
                     /locus_tag="VI319_06630"
     CDS             8218..8562
                     /gene="sdhD"
                     /locus_tag="VI319_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY6863560.1"
                     /translation="MKRVVVGAHYGLGGWMVQRVSAAYMALYTVLFVALLLASGPPSY
                     ASWRALFAPGWMRFATFLFAASVLAHAWVGMRDIWMDYIKPTGLRFALEAATALLLVG
                     CGAWALQILWRI"
     gene            8563..10326
                     /gene="sdhA"
                     /locus_tag="VI319_06635"
     CDS             8563..10326
                     /gene="sdhA"
                     /locus_tag="VI319_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY6863561.1"
                     /translation="MALIRRSFDVVVVGAGGAGLRASLQLAEAGFRVAVLSKVFPTRS
                     HTVAAQGGIGAALGNMGEDSWLWHMYDTVKGSDWLGDQDAIEFMVREAPRAVYELEHY
                     GMPFDRNDDGTVYQRPFGGHSMNFGERPVQRSCCAADRTGHAMLHTLYQRNVRANTQF
                     FVEWMALDLIRDAGGRVLGVTALEMETGEVSLLQARATLFATGGAGRIYASTTNAFIN
                     TGDGLGMAARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGYLLNKNGERFMERY
                     APNLKDLASRDVVSRAMATEIKEGRGAGQHADYLLLKLDHLGRETIEKRLPGIAEIAK
                     KFANVDPVKDPIPVVPTAHYQMGGIPTNFRGEVIVPGRGGPETVVPGFYAAGEVACAS
                     VHGANRLGTNSLTDLLVFGKASAESIDKFLRENPGTPDLPKDAGDASLARLARLDGQK
                     GGERVADVAHEMRRTMQAHCGVFRFPDLLASGVKKIFEVCDRATRTEIQDKSKVFNTA
                     RVEALELDNLVEVARASMVSAEARKESRGAHDRDDFHKRDDVNWLKHTLWYKDGDRLE
                     YKPVHLKPLTAASIEPKVRTY"
     gene            10326..11024
                     /locus_tag="VI319_06640"
     CDS             10326..11024
                     /locus_tag="VI319_06640"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY6863562.1"
                     /translation="MRFRIFRYDPEKDAKPYMKDYDVQLDPHDRMLLDALVRIKEQVD
                     DSLALRRSCREGVCGSDAMNINGKNGLACLTKLADLKEPVELRPLPGLPVIRDLIVDM
                     TQFFNQYHSITPYLQNHDPAPEKERLQSPEERDQLNGLWECVLCACCSTSCPSFWWNP
                     DKFVGPAGLLWAYRFIADSRDQATGERLDNLEDPYRLFRCHTIMNCVDACPKGLNPTR
                     AIGHIKELMVKRAV"
     gene            11030..11287
                     /locus_tag="VI319_06645"
     CDS             11030..11287
                     /locus_tag="VI319_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY6863563.1"
                     /translation="MEAAELRRLEWRCRRGLLENDLVLRAFLDRHGPGLAGERLRAFR
                     VLLEYSDNDLWDLVSGRAECADPALCAVVEELRRCQIRETQ"
     gene            11284..12582
                     /gene="gltA"
                     /locus_tag="VI319_06650"
     CDS             11284..12582
                     /gene="gltA"
                     /locus_tag="VI319_06650"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY6863564.1"
                     /translation="MTSSKKAVLQYGDGKSLEFPVLDGSIGPEVIDIRTLYGKTGMFT
                     YDPGYLSTASCSSKITYIDGDQGVLLYRGYPIEDLATRCDFLEVCYLLQYGELPTQKQ
                     KDEFVNIVTHHTMVHEQLARLYQGFRRDAHPMAVMVGVVGAMSAFYHDALDITNPQHR
                     VISAFRLVAKMPTIVAMSYKYSVGQPFVYPRNDLSYTANFMRMMFAVPAEEYKVNDVL
                     VRAMDRIFILHADHEQNASTSTVRLCGSSGANPYACIAAGIASLWGPAHGGANEAALQ
                     MLEEIGDVSKVGDFIKRVKNKEPGVRLMGFGHRVYKNYDPRAKLMRETCHEVLDELGL
                     HDDRIFKLAIALEKIALEDDYFVSRKLYPNVDFYSGIVQRALGIPVAMFTCIFSLART
                     IGWIAQWQEMISDPEYKIGRPRQLYTGAPRREFVPIDKRK"
     gene            12637..>14894
                     /locus_tag="VI319_06655"
     CDS             12637..>14894
                     /locus_tag="VI319_06655"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY6863565.1"
                     /translation="MSGMKDFLSNSYIFGANAPFIEGLYESYLDNPQSVPEQWREHFD
                     KLQLLPGAGDGRGRDVAHAPIVESFAQRAKQNKLRAAESPTELSVERKQVYVLLLIAA
                     YRTRGSRWAELDPLKRQQRPQIPELEPAFYDLTEADMETVFNTGTLVGPERMTLREIL
                     KALRETYCSSIGVEYMYMSDTAQKRWIQQRLESVRSHPSLGPEVKKRILERLTAAETL
                     EKYLHTRYVGQKRFSLEGGESLISSMDEVVQRAGSHGVQELVIGMAPRGRLNVLVNTL
                     GKNPKELFSEFEGKHHSELSAGDVKYHNGFSSDISTSGGPIHLSLAFNPSHLEIVNPV
                     VEGSVRARQRRRKDVKGDQVLPLLIHGDASFAGQGVVMETLNLSSTRGYGTAGTVHIV
                     VNNQIGFTTSDPRDARSTIYCTDVAKMVEAPIFHVNGDDPEAVHFVTQIAMDYRKEFR
                     RDVVVDIVCYRRLGHNEQDEPLVTQPLMYKKIAQTPGTRKLYSDKLIAQKVIDPEAPE
                     QMIKDYRQALEEGRHTSSPVLSNFQRNFAVDWSPFMNAKWTDAADTSVPLEELQKLAE
                     RITTVPAGFKLHPTVERVMQARREMGQGKKMLDWGMAENLAYATLLVNGYAVRLSGQD
                     SGRGTFAHRHAVLHDQNREKWDAGTYVPLEHLKPDQPSFEIIDSVLSELGVVGFEYGY
                     STSDPARLVIWEAQFGDFANGAQVIIDQFISSGEVKWGRICDLVMLLPHGYEGQGPEH
                     SSARPERYLQLCA"
BASE COUNT         2330 a         4999 c         5070 g         2495 t
ORIGIN      
        1 ttgcggttgc ccgagagcac cgacgcgcac acgaggtcgc tcttgttgac ggtctcctcg
       61 atcgtctcgg cgagcgggcc ggagttgccg atgcaggtgg tgcagccgta cgcggagaga
      121 tagaagccga gcttctcgag atagggcagc aggccggtct tcgtcaggta gtcggtgacg
      181 acgcgcgatc ccggcgcgag agacgtcttc acgtgcttct tgacggtcag cccgcgctcc
      241 accgccttct tcgcgaggag cccggccgcg agcaggacgc ccgggttgga ggtgttcgtg
      301 cacgaggtga tcgccgcgat cagcacgtcg cccgagccga gctcgatgcc gtcgcgggtc
      361 ttgaagcgct tcgcgaggtc ctcggggcgc ttcgcgaagc ccgcctcggc tacgggcttc
      421 gagaacagct cgccgaactt gcccttcacc ttgccgatct cgatgcggtc ctgcgggcgc
      481 ttcgggccgg cgaccgacgg cgcgacggtc gcgaggtcga gctcgaccac ctcgctgtag
      541 tcgatctgcc ccggcttcgg cacgccgtac atgccctgcg ccttgaagta ggacctgagc
      601 gcgtcgacct cgtccttgct gcgcccggtg ccctcgaagt aggcgatggt ctggtcgtcg
      661 accgggaaga agcccatggt cgcgccgtac tcgggcgcca tgttgccgag cgtggcgcgg
      721 tcggggacgg tcaggctctc ggcgccttcg ccgaagaact cgacgaactt gccgacgacc
      781 ttggccttgc gcagcatctc ggtgacggtg agcacgaggt cggtcgcggt gacgccgccg
      841 cgcagccgcc ccttgaggtg cacgccgacc acgtccgggg tgaggaagta attcggctgg
      901 ccgagcatcc cggcctcggc ctcgatgccg cccacgcccc aggcgacgac gccaatgccg
      961 ttgatcatag tggtgtggct gtcggtgccg accaggctgt cgggatagta gatcccgtcc
     1021 ttctcgtgca cgccgcgcgc gagatattcg aggttgacct ggtggacgat gccgatgccg
     1081 ggcggcacga ccttgaacgt gtcgaaggcc tgcatcgccc atttcatgaa ctggtagcgc
     1141 tccttgttgc gctcgaactc gatgcgcatg ttcaggtcga gcgcgttctt cgcgccgtag
     1201 tggtcgatct ggaccgagtg gtcgacgacc aggtcgaccg ggaccagcgg ctcgatcacc
     1261 ttgggatcct tgccgacctt ctccgcgacg ttgcgcatgg cggcgaggtc gaccagcgcc
     1321 gggataccgg tgaagtcctg gagcaggatg cgcgcgagca cgaacgggat ctcctcggtg
     1381 cgcgccgcct ttgccttcca cgaggcgagc tggcgcacgt ggtcctcggt gaccttcttg
     1441 ccgtcgcagt ggcgcagcac cgcctcgagc acgatgcgca gggaaacggg gagccgggac
     1501 accttggcgc cgagcgcttt ctcgagcgcc ggcagggaat agagcttccc tttctttccc
     1561 gacgccatct tgaattcctg gagggtgttg aacaggttgt gggccatggc gggttcggat
     1621 gacgagtcag ttaacgaaca gatcgacgaa ttgatcgacg ggcgtcgcct cgaggcgctt
     1681 cgcgtcctgg cacaggtcga ggatctgctt ctgccgggct tcggggaagc ggcgcgcgag
     1741 gttcgcgcgg aacttcgctt ccagggccgg gatgccttcg cggcggcggc gacggtgacc
     1801 gagcggatat tctaccgcca ccttcggggt cgcgccgccg cccctgaaga agatctggat
     1861 ctggttcgcg atcgagcgct tcttcggatc gagatagtcc ttcgaccagc gcttgttctc
     1921 gatgcagacc atcttcgcgc gcagcgcgtc gatgcgcgga tcccgcgcga cggcgtcctc
     1981 gtagtcggcc gcggtcaggg ctcccttgag cagtccgacg gcgaccatgt actggatgca
     2041 atggtcgcga tccgccgggt tgtgcaaagg ccccttcttg tcgatgatcc ggatcgccga
     2101 ttcgtgggtg acgatctcga tcttcttcac gtccgcgagg cggtcgcgga ccttcgggtg
     2161 cagcgcgatg gcggcttcca ccgcggtctg cgcgtggaac tcggccggga aggagatctt
     2221 gaagagcacg ttctccatca cgtaggagcc gtacttccgc gtgaatttga acggcttgcc
     2281 cctgaacagg acgtcgtaga agccccaggt cttggcgctg agcgccgagg ggtagcccat
     2341 ctcgccggcg cgcaccatca gcgcgagccg caccgcgcgc gcggtcgcgt cgcccgctgc
     2401 ccacgacttg cgcgagccgg tgttcggggc gtggcggtag gttcgcaggc tctggccgtc
     2461 gatccaggcg ttcgagaccg cgtcgatgat ccggtcgcgg tcgaggccga gcatcttcga
     2521 gacgaccgcg gcggaggcga ccttcaccag gaccacgtgg tccagcccca cccggttgaa
     2581 gctgttctcg agcgcgatca cgccctggat ctcgtgcgcc ttgatcatgg cggtcagcac
     2641 gtcgcgcacg acgagcggct cgcggcggtc ggcgcccgcg gtgcgcgaca gccagtcggc
     2701 gaccgcgagg atgccgccga gattgtccga gggatgtccc cattcggcgg cgagccaggt
     2761 gtcgttgaaa tcgagccagc ggatcatcgc gccgatgttg aacgccgcgt tgaccgggtc
     2821 gagctggtag ctcgtgcccg gcacgcgcgc gccattcggg atcacggtcc cgggaacgag
     2881 cggcccgagg agcttcgtgc acgccgggta ctcgagcgcc tcgagcccgc agcccagcgt
     2941 gtccatcagg cacaggcgcg ccgtctcgta ggcttcggcg ctctcgatct cgtgccgggc
     3001 gacgtaatcg gcgatatcga cgaggacttt gtccggcttc ggccggacat tcgagatggg
     3061 agcggacatc cctattgctt cttctcgggc tcctgcggcg tgggggcctg cggcggcgcg
     3121 gcgttctggg gcgcggcctc ctgcggagcg gcttcctgcg gcgcggcgcc cgagggcggc
     3181 acggcgagcg ttccctgcgg gttcgcggcg ccgggcgagg actcggtcac ggggcggctc
     3241 gccgaggagg gcaggcgcag gcatcggtac tccgccttcg cctcgggcca gcgcagcgcc
     3301 atgctcgcgg tgcgctgccg ggcgtcggcc tcgggcttca cgctgaactc ctggcctttc
     3361 gccggcttca ccttcagcca gccgtccgga tcctgcgcga gctcggcgcg gccgtagacc
     3421 tcgcgcgaga agccgtccac ctcgaccgtg accgccgacg cgccgccgcc gggcgcgtcc
     3481 gtgaagtaca ggatcggcgg cttgttgccg tagttcacgc cgggctggcg gcattcccag
     3541 gttccggcga tgtcggcggg cgcggcgacg gcggtgccgg tggcgcagag gacgccgaag
     3601 cacgcgagct tgaatcgtgt catggcctac tccctgttgg gatcaccggc ggtccttgag
     3661 cggcacccag cgctggtcct cggggccggt gtagttcgcg cccgggcgga tgattttacc
     3721 gtcgatccgc tgctcgatga tatgcgcgga ccagcccgag gttcgcgaga ttacgaagat
     3781 cggcgtaaac atcgccgtgg gcacgcccat cacgtggtag gacaccgcgc tgtaccagtc
     3841 gaggttggcg aacatcttct tctcgcgcgc catcaccgcc tcgaggcgct cggcgatgtc
     3901 gtagagcttc gtgtcgccgg cggacttcga aaggcgcttc gccaccgcct tgatcacctc
     3961 gttgcgcggg tcggcgatgg tatagaccgg gtggccgaag ccgatcacga tctccttgtt
     4021 gccgatgcgg cgcacgacat cggcctcggc ctggtcgggc gtgtcgtagc gcgactgcac
     4081 ctcgaacgcg acctcgttcg cgccgccatg cttggggccg cgcagcgcgc cgatgccgcc
     4141 gcagatcgcc gagtacatgt ccgaaccggt gcccgcgacc acgcggcacg tgaacgtgga
     4201 ggcgttgaac tcgtgctcgg cgtagaggat gagcgaggtg tgcatcgccc gcacccactc
     4261 ggccgagggc gccttgccgt ggagcagatg caggaagtgc ccgccgatcg agtcgtcgtc
     4321 ggtctcgacc tcgatgcggc ggccgttgtg gctccagtgg taccaataga gcagggcgga
     4381 gccgaggcac gccatcagac gatcggcgat gtcgcgcgcg cccggcacgt tgtggtcgtc
     4441 cttttccggg agggtcgttc cgagcaccga cacggcggtg cgcatcacgt ccatcgggtg
     4501 ggccgacgcg ggcacgactt cgagcgccgc cttgaccgcc acgggcagcc cgcggagcgc
     4561 cttcagcttc ttcttgtagc ccgcgagctc ggccgcggtc ggcagcttct cgtgcaccag
     4621 caggtgcgcg acctcctcga actcggcctg gtcggcgaag tcgaggatgt cgtagccgcg
     4681 gtagtgcagg tcgttgcccg tgcggccgac ggtgcagagc gccgtgttgc cggccgggac
     4741 gcccgacagg gcgacggact tcttgggctt cggagccatg gtgagatcgc tcatttgtcc
     4801 ttccctttcg cgaacagctg gtcgagcttc ttctcgtacg aatgatagtc gagatagtcg
     4861 tagaggtcgg cgcgcgtctg catctgtccg acgacgttct tttgcgtgcc ttccttgcgg
     4921 atcgcctcgt acaccccgag cgccgcggcg ttcatcgcgc ggaacgccga cagcggatag
     4981 agcgcgatcg cgacgttcgc cgagcgcagc tcgtcgagcg tgaacagcgg cgtcgatccg
     5041 aactcggtga tgttggcgag caccggcacc ttcaccgcgt cggcgaacct gcggtacatg
     5101 gcgaggtcgg tcatcgcctc ggggaagata gcgtcggcgc cggccgcgac gcacgcgacc
     5161 gcgcgctcga tcgcgcgctc gaggccttcg acggcgagcg cgtcggtgcg cgccatgacc
     5221 aggaaatccg ggtcggtctt cgcgtcgacc gccgccttga cgcggtcgac catctcttcc
     5281 ttcgcgacca gctccttccc gggacggtgg ccgcagcgct tcgcgccgac ctggtcctcg
     5341 atatgcatcc ccgcggcgcc ggccttgatc agggacttca cggtgcgggc gatgttgaac
     5401 gcgctcgcgc cgaagcccgt gtcgacgtcc accagcagcg gcagggcgca cacgtcggtg
     5461 atccggcgca cgtcgatgag cacgtcctcg agattgctga tgccgaggtc gggcaggccg
     5521 agcgagccgg ccgcgacgcc gccgcccgaa agatagatcg atcggtagcc gacccgctcc
     5581 gcgaggcggg cgtggtaggc gttgatcgtg ccgacgcact ggagcggctt ctcggcctgc
     5641 atcgcggcgc gcagccgcgc gcccgccgaa gctgcgctca tggctgtcgc tccgtctcga
     5701 agaacgcgcg cgcggcgtcc tcggggatcg ggacgccggt caccttcgcc ttctgcagga
     5761 cttcccagta gtagcggtac gaggcgcggt cgtggagctc gcccgcgtac tggatcgggc
     5821 cccagtcggc gcgctgcgcc gcgagcagga tcccggccgc gtcccgcacc tcggagaagt
     5881 cgggcttcat cgcgtcgacg atcggctgga tctgcgtcgg gtggatgctc cacatgcgca
     5941 tgaagccgta ttcctggcgc gcgcgccacg cgtcctcgta ggtgacgtac gcgttcttca
     6001 ggtcctgggt gacgttgtgc gtgggcacga tgccgttgcc gagcgccgcg gcggcgacgc
     6061 gcgccttcgc gcgcgcgagc agcttgtgct cgaactggcc gggcgagcgc atcgccgagg
     6121 cggggatcgc gccgtggtgg ctggagacga agtccatctg gccgaacgcg agcgcctgca
     6181 tccacggcaa acccgcgatc tcgtaggcct cgcgcagccc gccgtgcgtc tcgatcagcg
     6241 cgtggatggg gatctcgcgc cggatgccgt gatgcgcggc cacctgctgg acgtagccga
     6301 tcatgtcggc gacctgggcg gcggaggtcg ccttcggcag ggtgatgtag gcgagcagct
     6361 ccccggcgcc ttcgaccagg atgtcgacgt cgcggcgcca gtgcgggtgg ctgaagtcgt
     6421 ggatccggac cccggcgtgc cggaagcggt tcgcgggcga ggcgagcatg cgcacgatca
     6481 tctcggcgtg ctcgcgctcg cggccgggcg ctgcgccgtc ctcgcagtcg caggtgatgt
     6541 cgaagatcgg tccgagcctg tgctggatct cgaacgcctt gccgatgaag ttctcgttcc
     6601 ccgcgaagtg ctcgcaggac gggatcacgg gaaacggccg ctcccccttg aacaaggccg
     6661 cgtgggggtg gagcggcttg gacacgggtc tgtctctatc cgaggagcgc cttgatgccg
     6721 tcgcgctcct cctgcagctc cttgagcgtc gcgtccatgc gcttgcgcgc gaagtcgctg
     6781 atgtcgaggc ccttgacgat ctcgtacttc ccgccacggc aggtgaccgg atagccgtac
     6841 aggacgccct ccgggatgcc gtagctgccg tcggacggga tgcccatcga gacccagtcg
     6901 ccctcgcgcg ttccgtgcac ccagtcgcgc acgtggtcga tcgcggcgtt tgccgcgctc
     6961 gccgccgacg acaggccgcg cgcctcgatg atcgcggcgc cgcgcttctg gacggtcggg
     7021 atgaagctgc tctcgagcca ttgctggtcg ttgatcatcg gccagacttt tttcccgtcc
     7081 acctcggcgt ggaagacgtc ggggtactgg gtcgacgaat gattgcccca gaccgcgacc
     7141 ttgcgcaccg acgcgatcgg cttgccgatc ttctgcgcga tctgcgacac ggcacggttg
     7201 tggtcgaggc gcatcatcgc ggtgaactgc gcgggcttca ggctcttcgc gttcttcatc
     7261 gcgatcaggc aattggtgtt cgcgggattg ccgacgacga gcacgcgcac gtcgcgcttc
     7321 gcgacccggt cgagcgcctg gccctgcggc gcgaagatct tgccgttcgc ctcgagcagg
     7381 tccttgcgct ccatgccggg accgcgcggc cgcgcgccca ccaggagcgc gacgtcgacg
     7441 tccttgaacg cgaccatcgg atccgaggtc gccgccatgc ccgcgagcag cgggaaggcg
     7501 cagtcgtcga gctccatcat cacgcccttc agcgccttct gcgccttctc gtccgggatc
     7561 tcgagcagct gcagcacgac cggctggtcg gcgccgagca tcgcgccgct cgcgatgcgg
     7621 aacagcaggc tgtagccgat ctggccggcg gcgccggtga cggccacgcg catcggtgac
     7681 ttggacatgg agagttcctc gagactgggg cgagagacgg aagacgcgat tatccccgac
     7741 tcttttgcag ggcacaaatc gacgccgacc ctgctataat cttgattctc gtcaagactt
     7801 caataagcca gcgaaccaag gatacggtta catgcctgat ctggcggcga aaagaaaacg
     7861 cccagtctgg ttcaacctga gtcttctcaa gcttcccgct cccggcctgg tctcgatctt
     7921 ccaccgcgcg agcggcgcgt ggctgttcct cggcctgatc tggttcctgt tcctcctcga
     7981 gatgagcctc ggctcggagg ccggctacga gcacgcgcgc gcctacgtcg cgcacccggt
     8041 cgtgaagctc gcgctgctcg cgttcctgtg gtcgtacctg caccacttct gcgccggcat
     8101 ccgctatctc ttcctcgaca tggacgtcgg cctgccgctc gcggcggcgc gccgcagcgc
     8161 cttcgtcgtg ttcgcagtga gcctcgcgct cacggtcttg atcggagcgc ggctgtggtg
     8221 aagcgagtcg tcgtcggcgc gcactacggg ctcgggggct ggatggtcca gcgcgtgtcg
     8281 gcggcgtaca tggcgctgta tacggtgctt ttcgtcgcgc tgctgctcgc ctcgggcccc
     8341 ccgagctacg cctcgtggcg cgccctgttc gctcccggct ggatgcgctt cgccacattc
     8401 ctgttcgcgg cgagcgtgct cgcgcacgcc tgggtcggga tgcgcgacat ctggatggat
     8461 tacatcaagc cgaccggtct gcgcttcgcg ctcgaagcgg cgaccgcgct cctgctcgtc
     8521 ggctgcggcg cctgggcgct gcagattctc tggaggatct gagtggcgct catccggcgc
     8581 agcttcgatg tcgtggtggt cggcgcgggc ggcgccggcc tgcgcgcctc gctccagctc
     8641 gccgaggccg ggttccgcgt cgcggtgctc tcgaaggtgt tcccgacgcg ctcgcacacg
     8701 gtcgcggcgc agggcggcat cggcgcggcg ctcggcaaca tgggcgagga ctcgtggctg
     8761 tggcacatgt acgacacggt caagggatcg gactggctcg gcgatcagga cgcgatcgag
     8821 ttcatggtgc gcgaagcccc gcgcgcggtg tacgagctcg agcactacgg gatgccgttc
     8881 gaccgcaacg acgacggcac cgtgtaccag cgtcccttcg gcggccactc gatgaatttc
     8941 ggcgagcgcc cggtgcagcg ctcgtgctgc gccgccgacc gcaccggcca cgcgatgctg
     9001 cacacgctgt accagcgcaa cgtgcgcgcc aacacccagt tcttcgtcga gtggatggcg
     9061 ctcgacctga tccgcgacgc gggcgggcgg gtgctgggcg tcaccgcgct cgagatggag
     9121 acgggcgagg tgtcgctgct ccaggcgcgc gcgaccctgt tcgcgaccgg cggcgccggg
     9181 cgcatctacg cgtcgaccac caacgccttc atcaacacgg gcgacgggct gggcatggcc
     9241 gcgcgcgcgg ggatcccgct cgaggacatg gagttctggc agttccaccc gacgggcgtc
     9301 gcgggcgccg gcgtgctcat caccgagggc gtgcgcggcg agggcgggta cctgctgaac
     9361 aagaacggcg agcgcttcat ggagcgctac gcgccgaacc tcaaggacct cgcctcgcgc
     9421 gacgtcgtgt cgcgcgccat ggccaccgag atcaaggaag gccgcggcgc gggccagcac
     9481 gcggattacc tgctgctcaa gctcgatcac ctcggccgcg agaccatcga gaagcgcctc
     9541 ccgggcatcg ccgagatcgc gaagaaattc gccaacgtcg acccggtgaa ggacccgatc
     9601 ccggtggtgc ccaccgcgca ctaccagatg ggcgggatcc cgacgaactt ccggggcgag
     9661 gtgatcgttc ccggccgcgg cggcccggag accgtcgtcc ccggcttcta tgccgcgggc
     9721 gaggtcgcgt gcgcctcggt gcacggcgcg aaccgtctcg gcaccaactc gctcaccgac
     9781 ctgctggtct tcggcaaggc gtccgcggag tcgatcgaca agttcctgcg cgagaatccc
     9841 gggaccccgg accttcccaa ggacgcgggc gacgcgagcc tcgcgcgcct cgcgcgcctc
     9901 gacggacaga agggcggcga gcgcgtcgcg gacgtcgccc acgagatgcg ccgcacgatg
     9961 caggcgcact gcggcgtgtt ccgcttcccg gacctgctcg cctcgggcgt gaagaagatc
    10021 ttcgaggtct gcgaccgcgc gacgcgcacc gagatccagg acaagagcaa ggtgttcaac
    10081 acggcgcgcg tcgaggcgct cgagctcgac aacctggtcg aggtggcgcg ggcgtcgatg
    10141 gtgtcggccg aggcgcgcaa ggagagccgc ggcgcgcacg accgcgacga ctttcacaag
    10201 cgcgacgacg tcaactggct caagcacacg ctctggtaca aggacgggga ccggctcgag
    10261 tacaagccgg tgcacctgaa gccgctaacg gcggcgagca tcgaacccaa ggtgagaacc
    10321 tactgatgcg attccgcata ttccgctacg accccgagaa ggacgcgaag ccctacatga
    10381 aggactacga cgtccagctc gatccgcacg accgcatgct gctcgacgcg ctcgtgcgga
    10441 tcaaggagca ggtcgacgac agcctggcgc tgcgtcgctc gtgccgcgag ggcgtctgcg
    10501 gctccgacgc gatgaacatc aacggcaaga acggcctggc gtgcctcacc aagctcgccg
    10561 acctgaagga gccggtcgag ctgcgcccgc tccccgggct gcccgtgatc cgcgacctga
    10621 tcgtggacat gacgcagttc ttcaaccagt accactcgat cacgccatac ctgcagaacc
    10681 acgatcccgc gccggagaag gagaggctgc agtcgcccga ggagcgcgat cagctgaacg
    10741 ggctgtggga gtgcgttctc tgcgcctgct gctcgacctc gtgcccgtcg ttctggtgga
    10801 accccgacaa gttcgtcggc cccgccgggc tgctgtgggc ctaccgcttc atcgccgaca
    10861 gccgcgacca ggccacgggc gagcgactcg acaacctcga ggacccgtac cgcctgttcc
    10921 gctgccacac gatcatgaac tgcgtcgacg cctgcccgaa ggggctgaac ccgacgcggg
    10981 cgatcgggca catcaaggaa ttgatggtca agcgggcggt ctagcgtcga tggaagccgc
    11041 ggagctgagg agactggagt ggcgctgccg gcgcggtctg ctcgagaacg acctggtcct
    11101 gcgcgcgttc ctcgaccggc acggccccgg tctcgcgggc gagcggctgc gggcgttccg
    11161 ggtgctgctg gagtacagtg acaacgatct gtgggatctg gtgagcggcc gcgccgagtg
    11221 cgccgatccc gccctgtgcg ccgtcgtgga agagctgcgg cgctgccaaa ttcgggagac
    11281 gcaatgacga gctcgaagaa ggccgtgctc cagtacggcg acggcaaatc gctggaattc
    11341 ccggtgctgg acggcagcat cggtcccgag gtgatcgaca tccgaacgct ctacggcaag
    11401 accgggatgt tcacctacga cccgggctat ctctcgaccg cgagctgcag ctcgaagatc
    11461 acctacatcg acggcgacca gggcgtcctg ctctaccgcg gctacccgat cgaggacctg
    11521 gcgacgcgct gcgacttcct cgaggtttgc tacctgctgc agtacggcga gctgccgacg
    11581 cagaagcaga aggacgagtt cgtcaacatc gtcacgcacc acacgatggt gcacgagcag
    11641 ctggcgcgcc tttaccaggg gttccgtcgc gacgcgcacc cgatggccgt catggtcggc
    11701 gtggtcggag cgatgtcggc cttctaccac gacgccctcg acatcacgaa tccgcagcac
    11761 cgcgtgatct cggcgttccg gctggtcgcc aagatgccga ccatcgtcgc gatgtcgtac
    11821 aagtactcgg tcggccagcc gttcgtgtac ccgcggaacg acctgtcgta caccgcgaac
    11881 ttcatgcgca tgatgttcgc cgtcccggcg gaggagtaca aggtcaacga cgtgctggtg
    11941 cgcgcgatgg accgcatctt catcctgcac gccgaccacg agcagaacgc ctcgacgtcg
    12001 acggtgcgcc tctgcggctc ctcgggcgcg aacccgtacg cctgcatcgc cgcgggcatc
    12061 gcctcgctct ggggcccggc gcacggcggc gccaacgagg cggcgctgca gatgctcgag
    12121 gaaatcggcg acgtctccaa ggtcggcgac ttcatcaagc gggtcaagaa caaggagccc
    12181 ggcgtgcgcc tgatgggctt cggccaccgc gtctacaaga actacgatcc gcgcgcgaag
    12241 ctgatgcgcg agacctgcca cgaggtgctc gacgagctcg gcctgcacga cgaccggatc
    12301 ttcaagctcg cgattgcgct ggagaagatc gcgctcgagg acgactactt cgtcagccgc
    12361 aagctctatc cgaacgtcga cttctactcg ggtatcgtgc agcgcgcgct cggcatcccg
    12421 gtcgcgatgt tcacctgcat cttctcgctc gcccgcacca tcggctggat cgcgcaatgg
    12481 caggagatga tctccgatcc ggagtacaag atcgggcggc cgcgccagct ctacaccggc
    12541 gcgccgcggc gcgagttcgt gccgatcgac aagagaaaat aagcccgtcc ctcggcattg
    12601 cgaacggggc gtcgcgcccc catctggaga atcgacatga gcggaatgaa agacttcctc
    12661 tccaactcgt acatcttcgg ggcgaacgcc ccgttcatag aagggctgta cgagtcctat
    12721 ctggacaatc cgcagtcggt gccggagcag tggcgcgagc atttcgacaa gctccagctg
    12781 ctcccggggg ccggcgacgg ccgcggccgc gacgtcgcgc acgccccgat cgtcgagtcg
    12841 ttcgcccagc gcgccaagca gaacaagctg cgcgcggcgg agtcgccgac cgagctctcg
    12901 gtggagcgca agcaggtcta cgtcctgctc ctcatcgccg cctaccgcac ccgcggctcg
    12961 cgctgggccg agctcgatcc cctgaagcgc cagcagcggc cgcagatccc cgagctcgag
    13021 cccgccttct acgacctcac cgaggccgac atggagacgg tgttcaacac cggcacgctg
    13081 gtgggccccg agcgcatgac gctgcgcgag atcctgaagg cgctgcgcga gacctactgc
    13141 agctcgatcg gcgtcgagta catgtacatg agcgacacgg cgcagaagcg ctggatccag
    13201 cagcgactgg agtccgtgcg ctcgcacccg agcctcgggc ccgaggtgaa gaagcgcatc
    13261 ctcgagcgcc tcaccgcggc cgagacgctg gagaagtacc ttcatacccg ctacgtcggg
    13321 cagaagcgct tctcgctcga gggcggggag agcctgatct cgtcgatgga cgaggtggtt
    13381 cagcgcgccg gcagccacgg cgtgcaggag ctggtgatcg gcatggcgcc ccgcgggcgc
    13441 ctcaatgtcc tggtcaacac cctcggaaag aatcccaagg agctgttctc cgagttcgaa
    13501 ggcaagcatc acagcgagct ttctgcgggc gacgtcaagt atcacaacgg cttctcgtcc
    13561 gacatctcga cctccggcgg gccgatccac ctgtcgctcg ccttcaaccc gtcgcacctg
    13621 gagatcgtca acccggtagt cgaaggctcg gtgcgtgcgc gccagcgccg gcgcaaggac
    13681 gtgaagggcg accaggtgct gccgctgctg atccacggcg acgcgtcctt cgcgggccag
    13741 ggcgtggtca tggagacgct gaacctgtcc tcgacgcgcg gctacggcac cgcgggcacg
    13801 gtgcacatcg tcgtcaacaa ccagatcggc ttcaccacgt ccgatccgcg cgacgcgcgc
    13861 tcgaccatct actgcaccga cgtggcgaag atggtcgagg cgccgatctt ccacgtgaac
    13921 ggcgacgacc ccgaggcggt gcacttcgtg acccagatcg cgatggacta ccgcaaggag
    13981 ttccgccggg acgtcgtcgt cgacatcgtc tgctaccggc gcctggggca caacgagcag
    14041 gacgagccgc tggtcacgca gcccctcatg tacaagaaga tcgcccagac cccggggacg
    14101 cgcaagctct actccgacaa gctgatcgcg cagaaagtca tagatccgga agcccccgag
    14161 cagatgatca aggattaccg gcaggcgctc gaggaaggac gccatacctc gagcccggtg
    14221 ctctcgaact tccagcgcaa cttcgccgtc gactggtcgc cgttcatgaa cgccaagtgg
    14281 accgacgccg ccgacacgag cgtccctctc gaggagctgc agaagctcgc cgagcgcatc
    14341 acgacggtgc ccgcgggctt caagctccat cccacggtcg agcgcgtcat gcaggcgcgc
    14401 cgcgagatgg ggcaggggaa gaagatgctc gactggggca tggccgagaa cctcgcctac
    14461 gcgacgctgc tcgtcaacgg ctacgccgtg cgcctctcgg ggcaggactc gggccgcggc
    14521 accttcgcgc accggcacgc ggtgctgcac gaccagaatc gcgagaagtg ggacgccggt
    14581 acctacgtgc cgctcgagca cctgaagccg gaccagcctt cgttcgagat catcgactcg
    14641 gtcctgtccg agctcggggt ggtcggcttc gaatacgggt actcgacttc ggatcccgcg
    14701 cggctcgtca tctgggaagc gcaattcggc gattttgcga atggcgcgca ggtgatcatc
    14761 gatcagttca taagctccgg cgaagtgaag tggggacgca tctgcgatct cgtcatgctg
    14821 ctgccacacg ggtatgaagg tcagggaccg gagcattcct ccgcgcggcc cgagcgttac
    14881 ctgcagctgt gcgc
//
LOCUS       DATDMW010000149         4085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_541762, whole genome shotgun sequence.
ACCESSION   DATDMW010000149 DATDMW010000000
VERSION     DATDMW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541762"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VI319_06660"
     CDS             <1..516
                     /locus_tag="VI319_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6863566.1"
                     /translation="LNGKILTGRDAAHKRIEDMLSHGEKLPVDFTNRVIYYVGPVDPV
                     RDEVVGPAGPTTSTRMDKFTETMLAKTGLIGMIGKAERGPTAIEAIRKHKAVYLIAVG
                     GAAYLVSRAIRKSRVLAFEDLGMEAIYEFEVKDMPVTVAVDARGASVHQSGPQEWEKR
                     IRELKIPVTVA"
     gene            518..1144
                     /locus_tag="VI319_06665"
     CDS             518..1144
                     /locus_tag="VI319_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863567.1"
                     /translation="MSPTHTGAILLKCLGTPRELRSLSAAASALAAECVPLAAGSAGH
                     SAVESDEIYLYVYSREFGALGPAVPARVAERARALPAFAGLAITPVRLAPVFDSPGAS
                     RNVTALFHYVVETDVDDEANDADLNAWYDTEHMPGLAACPGTVRARRFRNPDGSPRYH
                     ACYDLVTKETLGSAPWLAVRHSAWSDRVRPHFANTKRTMFRRLFHVEL"
     gene            1141..2034
                     /locus_tag="VI319_06670"
     CDS             1141..2034
                     /locus_tag="VI319_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6863568.1"
                     /translation="MTALPRWLSIVLLLAIAASFGSNHIAARVAFDHGANVMTAVVFR
                     SIGTGLAVLGVLLALRIPLALPAPTAGRALVIGLVIAAQSWFLYSSVARLPVALALLT
                     FNSYPILYTLLAWATGLEPRPPRAALLAMPVALFGLALALDVVGGLDRLSSRWAEIGA
                     GAGFAVAASVSFALVLYLGARWMKGVDGRVRSAYGMASCGVAAFAAASATGTLVLPGD
                     GAGWLGLMLLTVFYGSSITALFVLQPRMRSASDVAVLNFEPVAVLFLAWAILGQALAL
                     KQVAGALIVIACIVAIGTARR"
     gene            2044..2928
                     /locus_tag="VI319_06675"
     CDS             2044..2928
                     /locus_tag="VI319_06675"
                     /EC_number="1.13.11.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HEY6863569.1"
                     /translation="MATILGGIGTSHVPTIGVAWDRKKQNDPDWAPLFKGYEPVAEWL
                     AQKAPDVLVFFYNDHATTFFFDHYPTFALGVGETYAIADEGLGPRPIPPLKGHAGLSR
                     HLAESLIEDEFDIATWQDLPIDHGCHSPLTMFWPPKPDWPGRIVPIEINVLQHPIPTP
                     MRCFKLGQAVRRAILSYPEDLKVVIVGTGGLSHQMNGERAGFNNEEWDLRFLDLIERD
                     PRALTAMRHADYIRLGGTEGAEIIMWLAMRGALSEGARKIHQNYYLPMTSAMAVAVFE
                     EPRTAAREPRAALSRERE"
     gene            2925..3284
                     /locus_tag="VI319_06680"
     CDS             2925..3284
                     /locus_tag="VI319_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423368.1"
                     /note="extradiol catechol dioxygenase that catalyzes the
                     oxidative cleavage of substituted catechols; part of the
                     bacterial aromatic compound degradation pathway; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEY6863570.1"
                     /translation="MNRQLQGVETLEGTYLFDLARSSRAMRLNRFLHGLTRPENRDLF
                     KENADAAFERAGLTAEERRMVRDLDWAALMRYGASFFCLEKLGRVRGVSNPEMVAGFR
                     GETLEQFLKTRRVPGAR"
     gene            complement(3302..>4085)
                     /locus_tag="VI319_06685"
     CDS             complement(3302..>4085)
                     /locus_tag="VI319_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6863571.1"
                     /translation="SVTEARAGRFDVDVIETNGPELESLHREKLLQKAGSPQFKDLLP
                     DAIRPHREWIGDRLNIFVQAYNTNLVKPADLPKTYQDLLDPKWKGRLGVEAEDIDWFA
                     TLVTAMGEQKGLQLFRDIVAKNGLSVRKGHSLLAGLVASGEVPFTLTVYNHNANKLKK
                     RGAPIDWYSIPPAIARVNGIALSRKPPHPNAAVLFYDFMLSPEGQAILVKGNYVPTNT
                     KTTGVDPKLTQLKLRFVDPAMILDQNAKWDKLYEDIITRQAK"
BASE COUNT          577 a         1455 c         1392 g          661 t
ORIGIN      
        1 ctgaacggaa agatcctcac cggccgcgac gccgcgcaca agcgcatcga ggacatgctc
       61 tcgcacggag agaagcttcc cgtcgacttc accaaccgcg tcatctacta cgtcggcccg
      121 gtcgacccgg tgcgcgacga ggtggtgggc cccgcgggcc cgaccacgtc cacgcgcatg
      181 gacaagttca ccgagaccat gctagcgaag accggcctga tcggcatgat cgggaaggcc
      241 gagcgcgggc cgacggcgat cgaggcgatc cgcaagcaca aggccgtgta tctcatcgcc
      301 gtcggcggcg ccgcgtacct cgtgtcgcgc gcgatccgca agtcgcgcgt gctcgcgttc
      361 gaggacctcg gtatggaggc gatctacgag ttcgaggtca aggacatgcc ggtgacggtc
      421 gcggtcgacg cgcgcggcgc gtcggtccac cagagcggtc cgcaggaatg ggagaagcgc
      481 atccgcgagc tcaagatccc ggtcaccgtc gcctgacgtg agcccgacgc acaccggagc
      541 gatcctcctg aagtgcctcg gaacgccgcg ggagctgcgc tcgctgtccg ccgcggcgag
      601 cgcgctcgcg gccgagtgcg tgcccctcgc cgcgggaagc gctggacact ccgcggtcga
      661 gagcgacgag atctatctgt acgtctactc gcgcgagttc ggcgcgctgg gccccgcggt
      721 tccggcgcgc gtggccgagc gagcgcgtgc cctcccggca ttcgccggcc tggcgatcac
      781 gccggtccgg ctcgcgccgg tgttcgactc cccgggcgcc tcgcgcaacg tgaccgcgct
      841 cttccactac gtcgtcgaga ccgacgtcga cgacgaagcg aacgacgccg acctcaacgc
      901 ctggtacgac accgagcaca tgcccgggct cgccgcgtgt ccggggacgg tgcgcgcgcg
      961 gcggttccgc aacccggacg ggtcgccccg ctaccacgcc tgctacgacc tcgtgacgaa
     1021 ggagacgctc ggcagcgcgc catggctcgc ggtgcgccac agcgcgtgga gcgaccgcgt
     1081 gcgtccgcat ttcgcgaaca ccaagcgcac gatgttccgg cgcctgttcc acgtcgaact
     1141 ttgaccgccc tgccccgctg gctgagcatc gtgctgctgc tcgccatcgc ggcgagcttc
     1201 ggcagcaacc acatcgccgc gcgcgtcgcc ttcgaccacg gcgcgaatgt catgacggcg
     1261 gtcgtgttcc gctcgatcgg aaccggcctc gcggtgctcg gcgtgctgct cgcgctgcga
     1321 attccgctcg cgctgccggc gccgacggcc gggcgcgcgc tcgtcatagg gctggtgatc
     1381 gcggcgcaga gctggttcct ctactcgtcg gtcgcgcggc tgcccgtggc gctcgcgttg
     1441 ctgaccttca atagctaccc gatcctgtac acgctgctcg cctgggcgac cgggctcgag
     1501 ccgcgccccc cgcgcgccgc gctgctcgcg atgccggtgg cgctgttcgg gctcgcgctc
     1561 gcgctcgacg tcgtcggagg cctcgaccgg ctctcgtcgc gctgggccga gatcggcgct
     1621 ggggcggggt tcgccgtcgc ggcctcggtg tcattcgcgc tggtgctcta cctcggcgcg
     1681 cgctggatga agggcgtcga cggccgcgtg cgcagcgcgt acggcatggc gagctgcggc
     1741 gtcgcggcgt tcgccgcggc gagcgcgacc gggacgctgg tcctgcccgg cgacggagcc
     1801 ggctggctcg ggctcatgct cctcaccgtc ttctacggct cgtcgatcac cgcgctgttc
     1861 gtgctccagc cgcgcatgcg ctcggcgagc gacgtcgcgg tgctcaactt cgagccggtc
     1921 gcggtgctct tcctcgcgtg ggcgatcctg ggccaggcgc tcgcgctgaa gcaggtggcg
     1981 ggcgcgctga tcgtcatcgc gtgcatcgtg gcgatcggca cggcgaggcg ctaggataag
     2041 cgcatggcga ccatcctcgg cggcatcggc acctcgcacg tccccacgat cggcgtggcc
     2101 tgggaccgca agaagcagaa cgatcccgac tgggccccgc tcttcaaggg ctacgagccg
     2161 gtcgcggaat ggctcgccca gaaggcgccc gacgtgctgg tgttctttta caacgaccac
     2221 gcgacgacct tcttcttcga ccactacccg accttcgcgc tcggcgtcgg cgagacctac
     2281 gccatcgccg acgaaggcct gggcccgcgc ccgatcccgc cgctcaaggg tcatgcgggg
     2341 ctctcgcgcc acctcgccga gagcctgatc gaggacgagt tcgacatcgc gacctggcag
     2401 gacctcccga tcgaccacgg ctgccactcg ccgctcacca tgttctggcc gccgaagccc
     2461 gactggccgg gccggatcgt gccgatcgag atcaacgtgc tgcagcaccc gatcccgacg
     2521 ccgatgcgct gcttcaagct cggacaggcg gtgcgccgcg cgatcctgtc gtatcccgag
     2581 gacctgaagg tcgtgatcgt gggcaccggc gggctctcgc atcagatgaa cggcgagcgc
     2641 gccggcttca acaacgagga gtgggatctg aggttcctcg acctgatcga gcgcgacccg
     2701 cgcgccctca ccgcgatgcg ccacgccgac tacatccgcc tcggcggcac cgaaggcgcc
     2761 gagatcatca tgtggctcgc gatgcgcggg gcgctctccg aaggcgcgcg caagatccac
     2821 cagaactact acctgccgat gacctcggcg atggcggtcg cggtgttcga ggagccgcgg
     2881 accgccgcgc gcgagccgcg cgcggctctt tcgcgcgagc gcgaatgaac cggcagctcc
     2941 agggcgtcga gacgctcgag ggcacgtatc ttttcgacct cgcgcgcagc tcgcgcgcga
     3001 tgcggctcaa ccgctttctc cacgggctca cacggcccga gaaccgcgac ctcttcaagg
     3061 agaacgccga cgcggcgttc gagcgcgccg gcctgacggc cgaggagcgc cgcatggtgc
     3121 gcgatctcga ctgggcggcg ctcatgcgct acggcgcgag cttcttctgc ctggagaagc
     3181 tcggccgcgt gcgcggcgtg tccaaccccg agatggtcgc cgggtttcgc ggcgagacgc
     3241 tcgagcagtt cctgaagacg cggcgcgtgc cgggcgcgcg ctgacgcgcc cggcgcgcga
     3301 ctcacttcgc ctgccgcgtg atgatgtcct cgtagagctt gtcccacttc gcgttctggt
     3361 cgaggatcat cgccgggtcg acgaacctga gcttcagctg ggtcagcttc gggtcgaccc
     3421 ctgtcgtctt cgtattcgtc ggcacgtagt tgcctttaac caggatcgcc tgcccctccg
     3481 ggctgagcat gaagtcgtag aacagcaccg cggcgttcgg gtgcggcggc ttcctcgaca
     3541 gcgcgatgcc gttcacgcgc gcgatcgcgg gcgggatcga gtaccagtcg atcggcgcgc
     3601 cgcgcttctt cagcttgttc gcgttgtggt tgtacaccgt cagcgtgaag ggcacctcgc
     3661 ccgaggcgac caggccggcg agcagcgagt ggcccttgcg caccgagagc ccgtttttcg
     3721 cgacgatgtc gcggaacagc tgcaggcctt tctgctcgcc catcgccgtc accagcgtgg
     3781 cgaaccagtc gatgtcctcg gcctcgacgc cgagcctccc cttccatttc gggtcgagca
     3841 ggtcctggta ggtcttgggc aggtcggccg gcttcacgag gttcgtgttg taggcctgca
     3901 cgaagatgtt gagccggtcc ccgatccact cgcggtgcgg acgaatcgcg tcgggcagca
     3961 ggtccttgaa ctgcggcgag cccgccttct gcagcagctt ctcgcggtgc agcgactcga
     4021 gctcgggacc gttggtctcg atcacgtcga cgtcgaagcg gcccgcgcgc gcctcggtga
     4081 ccgag
//
LOCUS       DATDMW010000150         4208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1663755, whole genome shotgun sequence.
ACCESSION   DATDMW010000150 DATDMW010000000
VERSION     DATDMW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663755"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..954)
                     /gene="rfbD"
                     /locus_tag="VI319_06690"
     CDS             complement(91..954)
                     /gene="rfbD"
                     /locus_tag="VI319_06690"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY6863572.1"
                     /translation="MLIGATGQLGGDLLRNNAEHEIDAPARGRLDLARPAEAAEYLRR
                     ARPDLVINCAAFHNVPLCETEPEQAFRVNCIAVRDIALACRDIGARLVTFSSDYVFRG
                     DRRTPYAEEDAPGPVQIYGITRLAGENAALAAAPEHAIVIRTCGLYGRSGAKSKGGNF
                     VDKVLELARRERAFDMSSDQTVAPTSTDDLSRAVLALIAHPGARPGIYHLVNEGECTW
                     HEFSRAIVELAGLGAEVRPVDRGGRTGDMRRPLYSVLANTRARALGIALRPWREALAD
                     YVAAKAAPRSA"
     gene            complement(960..1499)
                     /gene="rfbC"
                     /locus_tag="VI319_06695"
     CDS             complement(960..1499)
                     /gene="rfbC"
                     /locus_tag="VI319_06695"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY6863573.1"
                     /translation="MKFVATPLAGAFVVELEELADERGFFARSFCRDEFARHGLEPAV
                     AQCNVSYNAQRGTLRGLHYQAEPHPEAKLVRCTRGAVWDVAVDLRPGSPTLHRWFALE
                     LTADNRRALYVPAGFAHGFQSLTDSSEVLYQMSESYRAELARGVRWDDPRLAIPWPVA
                     PAIVSERDRALPLVGKAAR"
     gene            complement(1496..2737)
                     /locus_tag="VI319_06700"
     CDS             complement(1496..2737)
                     /locus_tag="VI319_06700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6863574.1"
                     /translation="MRSSRRCRFCAAPLELSFADLGMSPLSNANLRAAQLNAMEPFYP
                     LHAWACTSCFLVQLEQFESRERIFSDDYAYFSSYSDSWLAHCRAYTEAMIARFGYGAK
                     SLVVEIASNDGYLLQYFAQRGVPVLGIEPAANVARAAEAKGIPTRVEFFGTETAKRLA
                     AEGRRADLLLGNNVLAHVPDLNDFVRGMKELLAPGGVITMEFPHLLRLMAESQFDTIY
                     HEHFSYFSFATAEKVFAAHGITLFDVERIPTHGGSLRIYGRHADDAAKPVSQRVKDLL
                     RDEHDAGLARPGTYAAFAEQVKATKRALLRFLIDAKESGKRVAGYGAPAKGNTLLNYC
                     GVRSDLLEYTVDRSPHKQGLFLPGTHIPIHAPERIAETRPDYVLILPWNLKDEITAQM
                     AHVRSWGGRFVVPIPRATVLE"
     gene            complement(2734..3795)
                     /gene="rfbG"
                     /locus_tag="VI319_06705"
     CDS             complement(2734..3795)
                     /gene="rfbG"
                     /locus_tag="VI319_06705"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEY6863575.1"
                     /translation="MTNRDFWAGKRVFLTGHTGFKGSWLALWLHALGADVAGYALAPP
                     TEPSLWDRLGLATRVRDERADVRDLDRLMRALAGFRPEVVFHLAAQSLVRPSYADPVA
                     TYATNVLGTVHVLEAARRTDSVRVVVVVTSDKCYENREQDRGYTEEDAMGGHDPYSSS
                     KGCAELVTAAYRRSFFGKGAVVTSARAGNVIGGGDWAADRLVPDLVRAARSGLEARIR
                     NPNATRPWQHVLDPLHGYLILAERAWGDPALAGGWNFGPDERDAVPVGRIADEFASRW
                     GAGAAWAADGGEHPHEARYLKLDCAKARSRLGWAPRLPRAAALDWTVEWYRADAAGAD
                     VRALSLEQIRRYEERGERK"
     gene            complement(3792..>4208)
                     /locus_tag="VI319_06710"
     CDS             complement(3792..>4208)
                     /locus_tag="VI319_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY6863576.1"
                     /translation="LDGDFCMTYGDGVSNVDIAKAIAFHRAQRTHATLTAVQPPGRFG
                     ALVLEDRLITSFQEKPKGDGGWINGGFFVLSPQVLDTIDGDHTVWEREPLERLARERT
                     LAAWFHRGFWQPMDTLRDKNHLEELWASGSPPWKVW"
BASE COUNT          599 a         1478 c         1539 g          592 t
ORIGIN      
        1 gggatggcgc cggtacgact cgagaaagcg gcgcagcggc tcgacgccgt tccggcgtcg
       61 cacgaggtgc acgaccgcga tcgccggcat tcaggcgctc cgcggcgcag ccttcgccgc
      121 gacgtagtcg gcgagggcct cgcgccacgg acgcagcgcg atcccgagcg cgcgggcacg
      181 cgtgttcgcg agcaccgagt acagcggccg gcgcatgtcg cccgtgcgcc cgccgcggtc
      241 gaccggccgc acctccgccc cgagtccggc gagctcgacg atggcgcggc tgaactcgtg
      301 ccaggtgcac tcgccctcgt tcaccagatg gtagatgccc gggcgcgctc ccgggtgggc
      361 gatcagcgcg agcactgcgc gcgacaggtc gtcggtcgac gtcggcgcga cggtctggtc
      421 gctgctcatg tcgaaggcac gctcgcggcg cgccagctcg agcaccttgt cgacgaagtt
      481 gccgcccttg gacttcgccc ccgagcgtcc gtagaggccg caggtgcgga tgacgatcgc
      541 gtgctcgggc gccgcggcga gcgcggcgtt ctcgccggcg agccgcgtga tgccgtagat
      601 ctgcaccggc ccgggcgcgt cctcctcggc gtagggcgtg cgccggtctc cgcggaacac
      661 gtagtcgctg ctgaaggtca cgagccgcgc tccgatgtcg cggcatgcga gcgcgatgtc
      721 gcgcacggcg atgcagttga cgcggaaggc ctgctcgggc tcggtctcgc acagcggcac
      781 gttgtggaag gcggcgcagt tgatcaccag gtccgggcgc gcgcggcgga gatactccgc
      841 ggcttcggcc ggccgcgcga ggtcgagccg gccgcgcgcc ggcgcgtcga tctcgtgctc
      901 ggcgttgttg cgcaggagat cgccccccag ctgccccgtg gcgccgatca gcacgatcct
      961 caacgcgcgg ccttcccgac gagcggcagc gcgcggtcgc gctccgagac aatcgcgggc
     1021 gcgaccggcc agggaatcgc gaggcgcgga tcgtcccagc gcacgccacg ggcgagctcc
     1081 gcacggtagg actcggacat ctggtacagg acctcgctcg agtcggtgag cgactggaag
     1141 ccgtgggcga atccggccgg gacgtacagc gcccggcggt tgtccgcggt gagctcgagc
     1201 gcgaaccagc ggtgcagcgt cggcgagcct ggccgcagat cgaccgcgac gtcccacacc
     1261 gcgccgcgcg tgcagcgcac cagcttcgcc tcgggatgcg gctcggcctg gtagtggagt
     1321 ccgcgcagtg tgccgcgctg cgcgttgtac gagacgttgc actgcgcgac cgccggctcc
     1381 aggccgtggc gcgcgaactc gtcgcggcag aacgagcgcg cgaagaagcc gcgctcgtcg
     1441 gcgagctcct cgagctcgac cacgaacgcg cccgcgagcg gcgtcgcgac gaacttcatt
     1501 cgagcaccgt cgcgcgcgga atggggacga cgaagcgccc gccccaggag cgcacgtgcg
     1561 ccatctgcgc cgtgatctcg tccttcaggt tccacggcag gatcagcacg taatcgggac
     1621 gcgtctcggc gatgcgctcg ggcgcgtgga tcgggatgtg cgtcccgggg aggaacagcc
     1681 cctgcttgtg cgggctgcgg tcgaccgtgt actcgagcag gtcggagcgc acgccgcagt
     1741 agttgagcag cgtgttgccc ttggccgggg cgccgtagcc cgcgacgcgc ttgcccgatt
     1801 ccttggcgtc gatcaggaag cgcagcagcg cgcgcttcgt ggccttcacc tgctcggcga
     1861 aggcggcgta cgtgcccgga cgcgcgaggc ccgcgtcgtg ctcgtcgcga agcaggtcct
     1921 tcacgcgctg cgacacgggc ttcgccgcgt cgtcggcgtg ccggccgtag atgcggagcg
     1981 atccgccgtg cgtcgggatc cgctcgacgt cgaacagcgt aatcccgtgc gccgcgaaca
     2041 ccttctcggc cgtggcgaac gagaagtagg aaaagtgctc atggtagatg gtgtcgaact
     2101 ggctctcggc catcagccgc agcaaatgcg ggaactccat cgtgatgacg ccgcccggag
     2161 cgagcagctc cttcatgccg cgcacgaagt cgttcaggtc cgggacgtgg gcgagcacgt
     2221 tgttgcccag cagcaggtcg gcgcgccggc cctcggcggc gaggcgcttc gccgtttccg
     2281 tgccgaagaa ctcgacgcgc gtcgggatcc ctttcgcctc ggccgcgcgc gccacgttgg
     2341 ccgcgggctc gatcccgagc accggcacgc cgcgctgcgc gaagtactgc agcaggtagc
     2401 cgtcgttgct cgcgatctcg accacgaggc tcttcgcgcc gtagccgaag cgcgcgatca
     2461 tcgcctcggt gtacgcgcgg cagtgcgcga gccaggagtc ggaatacgag gagaagtagg
     2521 cgtagtcgtc ggagaagatg cgctcgcgcg actcgaactg ctcgagctgc accaggaagc
     2581 acgacgtgca cgcccaggcg tgcagcggat agaagggctc catcgcgttc agctgcgcgg
     2641 ccctgaggtt cgcgttggag agggggctca tcccgaggtc ggcgaacgac agctcgagcg
     2701 gcgcggcgca gaagcggcag cggcgcgagc tcctcatttg cgttcacctc gctcctcgta
     2761 gcgccggatc tgctcgagcg agagcgcgcg cacgtcggcg ccggccgcgt ccgcgcggta
     2821 ccactcgacg gtccagtcga gcgccgcggc gcgcggcagc cgcggcgccc agccgaggcg
     2881 cgagcgcgcc ttcgcgcagt cgagcttcag gtagcgcgcc tcgtgcgggt gctcgccccc
     2941 atccgcggcc caggcggcgc ccgcgcccca gcgcgacgcg aactcgtcgg cgatccggcc
     3001 gaccggcacg gcgtcgcgct cgtcgggtcc gaagttccag ccgccggcga gcgccgggtc
     3061 gccccacgcg cgctcggcga ggatcaggta gccgtgcagc ggatcgagca cgtgctgcca
     3121 ggggcgggtc gcgttcgggt tgcggatgcg tgcctcgagt cccgagcgcg cggcgcgcac
     3181 caggtcgggc acgagccggt ccgcggccca atcgccgccg ccgatgacgt tgcccgcgcg
     3241 cgccgaggtc acgacggcgc ctttgccgaa gaacgagcgc cggtacgccg cggtcacgag
     3301 ctcggcgcag cccttgctgc tcgagtacgg atcgtggccg cccatggcgt cctcctcggt
     3361 gtagccgcgg tcctgctcgc ggttctcgta gcacttgtcg ctggtcacga ccacgacgac
     3421 gcgcacgcta tcggtgcggc gcgccgcttc gagcacgtgc accgtgccga gcacgttggt
     3481 cgcgtaggtg gcgacgggat cggcgtacga cggccgcacc agcgactggg ccgcgagatg
     3541 gaacacgact tcgggacgga acccggcgag cgcgcgcatc agacgatcga gatcgcgcac
     3601 gtcggcgcgc tcgtcgcgca cgcgtgtcgc gagcccgaga cgatcccaga gcgagggctc
     3661 ggtgggcggg gcgagcgcgt agcccgcgac gtcggcaccg agcgcgtgga gccacagcgc
     3721 gagccagctg cccttgaagc cggtgtggcc ggtgaggaag acgcgcttac ctgcccagaa
     3781 atcgcggttc gtcaccagac cttccacggg ggcgacccgc tcgcccacag ctcctcgaga
     3841 tggttcttgt cgcgcagcgt gtccatcggc tgccagaagc cgcggtggaa ccacgccgcc
     3901 agcgtgcgct cgcgcgcgag gcgctcgagc ggctcgcgct cccagaccgt gtggtcgccg
     3961 tctatggtgt cgagcacctg cggcgacagc acgaaaaagc cgccgttgat ccagccgccg
     4021 tcgcccttcg gcttctcctg gaagctcgtg atcagccggt cctcgagcac gagcgcgccg
     4081 aagcggccgg gcggctgcac cgcggtgagc gtcgcgtgcg tgcgctgcgc ccgatggaac
     4141 gcgatcgcct tggcgatgtc gacgttcgag acgccgtcgc cgtacgtcat gcagaagtcg
     4201 ccatcgag
//
LOCUS       DATDMW010000151         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1041041, whole genome shotgun sequence.
ACCESSION   DATDMW010000151 DATDMW010000000
VERSION     DATDMW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041041"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..759)
                     /gene="pdsR"
                     /locus_tag="VI319_06715"
     CDS             complement(70..759)
                     /gene="pdsR"
                     /locus_tag="VI319_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HEY6863577.1"
                     /translation="MAKRIAIVEDDAAIRANYAEAVKKHGYEVSAYARRAEAMSAFRA
                     RLPDLAVIDIGLGDEPDGGFALCRDLRALAPALPIIFLTARDSDFDVVSGLRLGADDY
                     LTKDVSLPHLMARISALFRRIDALREPKVADEALERGPLRLDARRFTASWKERPVELT
                     LTEFWMVHTLAKFPGHVKDRDALMRDANLVVDDGTITSHIKRIRRKFAAVDPAFDQID
                     TVYGMGYRWKA"
     gene            996..1151
                     /locus_tag="VI319_06720"
     CDS             996..1151
                     /locus_tag="VI319_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863578.1"
                     /translation="MSESSSTATPEAVRPSPRIVDVIAYMALTFGIGIAVSMVLAGAV
                     LFLNSPV"
     gene            1341..>2938
                     /locus_tag="VI319_06725"
     CDS             1341..>2938
                     /locus_tag="VI319_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HEY6863579.1"
                     /translation="MSQHEAVPARRAQTRPWVDDFLTRMLAAFTIAVSVAVSGAMLLA
                     ARGARAQEPSVDPSDAKSGTLLLRDRAGATLTAPLLSTDVRIRVSGMVARAEVRQTFA
                     NPCGEWLEGVYVFPLPENAAVDHLRMRLGERTVEGEIRERGEAQRVYASARGAGQRAA
                     LLEQERPNIFTSSVANVGPDERVTVEIEYEQTLRYQALADGGARWSLRFPTVIGPRYI
                     PAGRLSEVRDAARVTDAARVTPPVLPPRRDEPPVNPVTIRVELDAGVPIATIDSPYHR
                     ITVQTLDERRAVIELADGATPATRDFELVWTPVLAQAPRAAWFTERRGDRSYGLLMLM
                     PPTAPRLASERLPREAIFVVDTSGSMVGTSISQAIEALEFAIDRLSPGDRFNVIEFNS
                     TARALFPDARPATRDNRATARGWVRALRAQGGTEMAKALDLALNGRQDPGRVRQVVFL
                     TDGQVGNEDELFRLIAGKLGDSRLFTVGIGSAPNSHFMTQAAKQGRGTFTYIGRIGEV
                     RDKMTELFAKLESPVLSGIRIDWP"
BASE COUNT          431 a         1056 c         1021 g          430 t
ORIGIN      
        1 cccacggcag cgcgagcagc agcagcgcga cgagcgcgag cttccagcgc aggctgatcc
       61 gcgggcgtct catgccttcc agcggtagcc catcccgtac accgtgtcga tctggtcgaa
      121 cgcgggatcg accgcggcga acttgcgccg gatgcgcttg atgtgcgagg tgatcgtgcc
      181 gtcgtcgacg accaggttgg cgtcgcgcat cagcgcgtcg cggtccttca cgtggcccgg
      241 aaacttggcg agcgtgtgca ccatccagaa ctcggtgagc gtcagttcga ccgggcgctc
      301 cttccagctc gcggtgaagc ggcgcgcgtc gagcctcaag ggcccgcgct cgagcgcctc
      361 gtcggcgacc ttgggctcgc gcagcgcgtc gatgcggcgg aagagcgccg agatccgcgc
      421 catcaggtgc ggcaggctca cgtccttggt gaggtagtcg tcggcgccga ggcgcaggcc
      481 cgacacgacg tcgaagtccg agtcgcgcgc ggtgaggaag atgatcggca gcgcgggcgc
      541 gagcgcgcgc aggtcgcggc agagcgcgaa gccgccgtcg ggctcgtcgc ccagaccgat
      601 gtcgatcacc gcgagatcgg gcaggcgcgc gcggaacgcg ctcatcgcct cggcgcggcg
      661 cgcgtaggcg ctcacctcgt agccgtgctt cttcaccgcc tccgcgtaat tcgcgcggat
      721 ggcggcgtcg tcctcgacga tggcgatgcg cttggccatg atgcgtccgg gattttttcc
      781 gcggcgcgcg acgcagcgtc gctgcgcggc gacatatttc acgaaaaatt ccgacgacta
      841 tagcgcaacg ccgcgcgctt tcgagcgcct gccatctttt tgccacaatt cttcggcatc
      901 atggcgtcat ggcgccggga tgcgatccgg ccggaacggt tgaatacagc tgtgtcgcac
      961 gggagagtgc ggccgcaaat ggaaaggaaa gagcgatgag cgagtcgagc agcaccgcca
     1021 cccccgaagc agtccgcccc agccccagga tcgtcgacgt catcgcctac atggcgctca
     1081 cgttcggcat cggcatcgcc gtgagcatgg tgctcgccgg cgcggtgctg ttcctgaact
     1141 ccccggtcta aggccaagcg tccccgcgag gccgggctcc gcctgaaggc gggcccggcc
     1201 tcttcatttc gggcgccatc ccggcgccac atttcttccc cgcttttgcg ccgcgcgcgc
     1261 acggcggggc ccttttccgg ccgtgtaatg cccggcatgg cccggcgacc cgccggccga
     1321 acggaaaggg agactcccgc atgtcgcaac acgaagccgt cccggcgcgc cgggcacaga
     1381 cgcgtccctg ggtggacgat ttcctcaccc gcatgctcgc cgcgttcacg atcgccgtct
     1441 cggtcgcggt gtcgggcgcg atgctgctcg ccgcgcgcgg ggcgcgcgcg caggagccct
     1501 cggtcgatcc gtccgacgcg aaatccggga cgctgctcct gcgcgaccgc gccggcgcga
     1561 cgctgaccgc gccgctgctc tcgaccgacg tgcgcatccg cgtctcgggc atggtcgcgc
     1621 gcgccgaggt gcgccagacc ttcgccaacc cctgcggcga atggctcgag ggcgtgtacg
     1681 tgttcccgct gcccgagaac gccgcggtcg accacctgcg catgcgcctc ggcgagcgca
     1741 cggtcgaagg cgagatccgc gagcgcggag aggcgcagcg cgtgtacgcg agcgcacgcg
     1801 gcgccggaca gcgtgccgcg ctcctcgagc aggagcgccc caacatcttc acctcgagcg
     1861 tcgccaacgt cggcccggac gagcgcgtga cggtcgagat cgagtacgag cagacgcttc
     1921 gctatcaggc gctcgccgac ggcggggcgc gctggtcgct gcgcttcccc accgtgatcg
     1981 gcccgcgcta catccccgcg ggccgcctgt ccgaggtgcg cgacgcggcc cgggtgaccg
     2041 acgctgcccg cgtcacgccc cccgtcctgc cgccgcggcg cgacgagccg ccggtgaacc
     2101 cggtgacgat ccgcgtcgag ctcgacgccg gcgtcccgat cgccaccatc gacagcccct
     2161 accaccggat cacggtgcag acgctcgacg agcgccgcgc ggtgatcgag ctcgccgacg
     2221 gcgcgacccc cgcgacgcgc gacttcgagc tggtgtggac gccggtgctc gcgcaggcgc
     2281 cgcgcgcggc ctggttcacc gagcgccgcg gcgatcgcag ctacgggctg ctgatgctga
     2341 tgccgccgac cgcgccgcgg ctggcgagcg agcgcctgcc gcgcgaggcg atcttcgtgg
     2401 tcgacacttc gggctcgatg gtcgggacgt cgatctcgca ggcgatcgag gcgctcgaat
     2461 tcgcgatcga ccgcctgtcg cccggcgacc ggttcaacgt catcgagttc aactccaccg
     2521 cccgcgccct gttccccgac gctcgccccg cgacgcgcga caaccgcgca acggcgcgcg
     2581 gctgggtgcg ggcgctgcgc gcgcagggcg gcaccgagat ggcgaaggcg ctcgatctcg
     2641 ccctgaacgg ccgccaggac ccgggccggg tacgccaggt cgtgttcctg accgacggcc
     2701 aggtcggcaa cgaggacgaa ctatttcggc tgatcgcggg aaagcttggc gactcgcggc
     2761 tgtttacggt gggcatcggc tcggcgccca acagccactt catgacgcag gcggcaaaac
     2821 aagggcgcgg cacgttcacc tatatcggca ggatcgggga ggttcgcgac aagatgaccg
     2881 agctatttgc caagctcgag tcgcccgtgc tctcgggcat ccgcatcgac tggccgga
//
LOCUS       DATDMW010000152         3727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_46332, whole genome shotgun sequence.
ACCESSION   DATDMW010000152 DATDMW010000000
VERSION     DATDMW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46332"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VI319_06730"
     CDS             <1..1455
                     /locus_tag="VI319_06730"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEY6863580.1"
                     /translation="RSGVSSNDMHVRPQAPGHGPLLAANDSQHTLVERQLAPVALAQA
                     MLDPAVVITALLACTAAFDQSFDAPYMILALIVFSLVFPGSVPRRSTLPGLAGDILAG
                     WLVVVGLLFLLGLATRTLGAFDPRVVYAWVLATPFAMLGAHVAFPRVLPRLLDAEGVQ
                     RTAIIAGGGELGRRLAERIRATPWLGIRVAGYFDDRTQDRITGIAPTEVLGNLQQLAS
                     YVKQNRVDLIYVTLPMASQPRIVKLLDELHDTTASIYFTPDIFLFHLIQARMDAIGGI
                     PVVAVCETPFYGINGLVKRASDIVLALAILLLISPLMLAIAIGVKRSSPGPILFRQRR
                     YGLDGREIVVYKFRSMKVLEDGNVIRQATRDDDRVTPLGAYLRRTSLDELPQFINVLQ
                     GRMSVVGPRPHAVAHNEMYRKLIKGYMIRHKVRPGITGWAQVNGLRGETETLDKMKER
                     IEYDLEYLRNWSLLLDLQIVVRTVFVVLRKQNAY"
     gene            1445..2875
                     /locus_tag="VI319_06735"
     CDS             1445..2875
                     /locus_tag="VI319_06735"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY6863581.1"
                     /translation="MPTDIAVYPVILSGGSGTRLWPMSRESMPKQFLPLLDGTTPFRA
                     TLARLRAVAGAKAPIVVANQDHRFLVLEQLREAGETPLAVYAEPAGRNTAPAAAIVAH
                     HLAQTDPDALMLVLPADHDIPDAEAFAEAVAAAATAARARRLVVFGIRARWPETGYGY
                     IERGEPLDAAPGCHGVASFIEKPELEIAERFVASGRHYWNSGMFLFGAEHFLSELARF
                     QPDVAAACEAAARSVRDDHGCMRIDAAQFGRCRSISIDYAVLEHTADAAVVPADFRWS
                     DIGSWNALWDASGKDAAGNVVRGDVMLSDVSGSYVCSTQRMVVGIGLKDTVVVETPDA
                     ILVADRSESQKVREMVEQLKARGRPEHKLHRRVHRPWGTYEDVDSGERFRVKRITVNP
                     GAKLSLQLHHHRAEHWVVVSGTARITRGEETLLLTENQSTYIPLGVRHRLENPGMIPL
                     QIIEVQSGAYLGEDDIVRFEDAYHRA"
     gene            2868..3065
                     /locus_tag="VI319_06740"
     CDS             2868..3065
                     /locus_tag="VI319_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863582.1"
                     /translation="MPRPLPPARFAVPAAWRAACLVVAALVVPDVADFVLDIGAGVAA
                     GALMLLYVRRKSETEAVCAES"
     gene            3050..>3727
                     /locus_tag="VI319_06745"
     CDS             3050..>3727
                     /locus_tag="VI319_06745"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674737.1"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEY6863583.1"
                     /translation="MCGIVSAIAPRNVVPVLIEGLKRLEYRGYDSAGVAVVNGRLHRL
                     RSAGRVAELERLAGGLSGEIGIAHTRWATHGVPSERNAHPHVSGGVSVVHNGIIENHE
                     AMRAKLIALGYEFTSDTDTEVIAHLVHARLAQGAPDLFAAVRQAVTELVGAYAIAVIC
                     ESDPARLVVARHGAPLLLGLGEGENFAASDTSALLQVTRRVIYLEDGDSAEVTLRGVT
                     IADRDGNV"
BASE COUNT          529 a         1376 c         1270 g          552 t
ORIGIN      
        1 cggagcggcg tgagctcgaa tgacatgcac gtgcggcccc aggctcccgg tcacgggccc
       61 ttgctcgccg cgaacgacag ccagcacacc ctcgtagaac ggcagctcgc gccggtcgcc
      121 ctggcgcagg cgatgctcga ccccgccgtc gtgatcacgg cgctgctcgc ctgcaccgcc
      181 gcgttcgacc agtcgttcga cgcgccgtac atgatcctgg cgctgatcgt gttctcgctc
      241 gtgttccccg ggagcgtccc gcgccgctcg acgctgccgg gactcgccgg cgacatcctc
      301 gccggctggc tggtcgtcgt ggggctgctg ttcctgctcg gcttggcgac gcgcacgctc
      361 ggcgccttcg acccgcgcgt cgtctacgcc tgggtgctcg ccacgccatt cgcgatgctc
      421 ggcgcgcacg tcgcgttccc gagggtgctg ccgcggctcc tcgacgccga aggcgtgcag
      481 cgcaccgcga tcatcgccgg cggcggcgag ctcggccggc gcctcgccga gcgcatccgc
      541 gccacgccct ggctcgggat ccgcgtcgcc ggctacttcg acgaccgcac gcaggaccgc
      601 atcacgggca tcgcgcccac cgaggtgctc ggcaacctgc agcagctcgc gagctacgtc
      661 aagcagaacc gcgtcgacct gatctacgtc acgctgccga tggcgtcgca gccgcgcatc
      721 gtgaagctgc tcgacgagct gcacgacacg accgcctcga tctacttcac gcccgacatc
      781 ttcctgttcc acctcatcca ggcgcgcatg gacgcgatcg gcggcatccc cgtcgtcgcg
      841 gtgtgcgaga cgccgttcta cggcatcaac ggcctggtca agcgcgcgag cgacatcgtg
      901 ctcgcgctcg cgatcctgct gctcatctcc ccgctgatgc tcgcgatcgc gatcggcgtg
      961 aagcgcagct cgcccggccc gatcctgttc cgccagcgcc gctacggcct ggacgggcgc
     1021 gagatcgtcg tctacaagtt ccgcagcatg aaggtgctcg aggacggcaa cgtcatccgc
     1081 caggcgaccc gggacgacga ccgcgtgacg cccctcggcg cctacctgcg gcgcacctcg
     1141 ctcgacgagc tgccgcagtt catcaacgtg ctccagggcc gcatgagcgt ggtcggcccg
     1201 cggccgcacg cggtcgcgca caacgagatg taccgcaagc tcatcaaggg ctacatgatc
     1261 cgccacaagg tgcgccccgg catcaccggc tgggcccagg tcaacggcct gcgcggcgag
     1321 accgagacgc tcgacaagat gaaggagcgc atcgagtacg acctcgagta cctgcgcaac
     1381 tggtcgctgc tgctcgacct gcagatcgtg gtgcgcaccg tgttcgtcgt actgaggaag
     1441 cagaatgcct actgacatcg ccgtctatcc ggtgatcctt tccggcggct cgggcacgcg
     1501 gctgtggccg atgtcgcgcg agtcgatgcc caagcagttc ctgcccctgc tcgacgggac
     1561 gacgccgttc cgggcgacgc tcgcgcgcct gcgcgcggtc gcgggcgcga aggcgccgat
     1621 cgtggtcgcg aaccaggacc accgcttcct cgtgctcgag cagctgcgcg aggcgggcga
     1681 gacgccgctc gcggtctacg ccgagcccgc cggacgcaac accgcgcccg ccgcggcgat
     1741 cgtcgcgcac cacctcgcgc agaccgatcc cgacgcgttg atgctggtgc tgcccgccga
     1801 ccacgacatc cccgacgccg aggcgttcgc cgaggcggtc gccgccgccg cgaccgctgc
     1861 gcgcgcgcgg cgcctcgtcg tcttcggcat ccgcgcccgc tggcccgaga ccggctacgg
     1921 ctacatcgag cgcggcgagc cgctcgacgc ggcgcccggc tgccacggcg tcgcgagctt
     1981 catcgagaag cccgagctcg agatcgccga gcgcttcgtc gcgtcaggac gccactactg
     2041 gaacagcggg atgttcctct tcggcgccga gcattttctg agcgagctcg cgcgcttcca
     2101 gcccgacgtc gccgccgcgt gcgaggccgc ggcgcgaagc gtgcgcgacg atcacggctg
     2161 catgcggatc gacgccgcgc agtttggacg ctgccgctcg atctcgatcg actatgccgt
     2221 gctggagcac accgccgacg cggccgtcgt tccggcggac ttccgctggt ccgacatcgg
     2281 ctcgtggaac gcgctctggg acgcctcggg caaggacgcc gcgggcaacg tcgtgcgcgg
     2341 cgacgtgatg ctctccgacg tgtcgggcag ctacgtctgc tcgacgcagc gcatggtcgt
     2401 cggcatcggc ctgaaggaca cggtcgtggt cgagacgccc gacgcgatcc tggtcgccga
     2461 ccggtccgaa tcgcagaagg tgcgcgagat ggtggagcag ctgaaggcgc gcgggcgccc
     2521 cgagcacaag ctgcaccgac gcgtgcatcg cccgtggggc acctacgagg acgtcgactc
     2581 gggcgagcgc ttccgcgtga agcgcatcac cgtcaatccg ggcgcgaagc tgtcgttgca
     2641 gctgcatcat caccgcgccg agcactgggt ggtggtgagc ggcaccgcgc gcatcacgcg
     2701 cggcgaggag acgctgctgc tcacggagaa ccagtcgacc tacatcccgc tcggcgtgcg
     2761 ccaccgcctc gagaaccccg ggatgattcc gctgcagatc atcgaggtgc agtcgggtgc
     2821 gtacctcggc gaggacgaca tcgtgcgctt cgaggacgcg taccaccgtg cctaggcccc
     2881 tgccgcccgc gcgcttcgcc gtgcccgccg cctggcgcgc cgcctgcctc gtggtcgcgg
     2941 cgctggtcgt gcccgacgtc gccgacttcg tgctcgacat cggcgccggc gtcgcggcgg
     3001 gcgcgctcat gctgctgtac gtgcggagga aatccgagac ggaggccgta tgtgcggaat
     3061 cgtgagcgcg atcgcgccgc gcaacgtcgt gccggtgctg atcgaggggc tgaagcgcct
     3121 cgagtaccgc ggatacgact cggcgggagt cgccgtcgtc aacggccggc tgcaccggct
     3181 gcgctcggcg ggccgcgtcg ccgagctcga gcggctcgcg ggcggcctgt cgggcgagat
     3241 cggcatcgcg cacacgcgct gggccacgca cggcgttcct tccgagcgca acgcccatcc
     3301 gcacgtctcg ggcggcgtgt cggtcgtgca caacggcatc atcgagaacc acgaggcgat
     3361 gcgcgcgaag ctgatcgccc tcggctacga gttcacctcg gacaccgaca ccgaggtgat
     3421 cgcgcacctc gtccacgccc ggctcgcgca gggcgcgccc gacctcttcg ccgcggtgcg
     3481 ccaggcggtc acggagctgg tcggcgccta cgcgatcgcg gtcatctgcg agtccgatcc
     3541 ggcgcgcctg gtggtcgcgc gccacggcgc gccgctgctc ctcggcctgg gtgaaggcga
     3601 gaacttcgcg gcgtcggaca cctcggcgct gctgcaagtg acccggcgcg tgatctacct
     3661 cgaggacggc gacagcgccg aagtcacgct gcgcggcgtc acgatcgcgg accgcgacgg
     3721 caacgtc
//
LOCUS       DATDMW010000153         4626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1289995, whole genome shotgun sequence.
ACCESSION   DATDMW010000153 DATDMW010000000
VERSION     DATDMW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289995"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VI319_06750"
     CDS             <1..242
                     /locus_tag="VI319_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6863584.1"
                     /translation="GFNFSPTIGVFAPAGTHKDVIGRIASDVAEAVKVPEVVTRLTNL
                     DIQPIGSTPEEYAADLRADDERYSKAVKISGAKVD"
     gene            251..1210
                     /locus_tag="VI319_06755"
     CDS             251..1210
                     /locus_tag="VI319_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863585.1"
                     /translation="MAAVVKPDRAAFSRELEALDVRPLWERVMRLKPGTAAVPAMWRW
                     AQMRPRLARASELITAKEAERRVLMLENPALRGMTFATTTLYAGLQIIMPGEIAPTHR
                     HTPNALRFIVEGEGAYTAVDGERVAMRPGDFVVTPNWTWHDHGNAGSEPVVWLDGLDT
                     AFANLFGAHFREDYPEERQPVAREDAASPLRYPYESTRAALARLAREGNPDPAHGHRL
                     RYRRADTGADPFPTMAVFLQHLPAGFSGRSYRSTDGAVFCVFEGRGTVVIGEKTFEFA
                     PHDVFVVPSWEAYRLAAAAETVLFSYSDRAAQETLGFWRESRE"
     gene            1207..2172
                     /locus_tag="VI319_06760"
     CDS             1207..2172
                     /locus_tag="VI319_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863586.1"
                     /translation="MSAVRPLAFAVALLACTGVFAQDYPARPVRVVVPSAPGGGFDLV
                     GRVLSQKLGEQTGQPFVVENRPGAGTLVGSQLVARAAPDGYTLLVGGLSNIALNMALY
                     KAPGYDSLADLAPVSLVVSHSYTLVGRKDLPQGTLREVIDFARANPGRLNMGTSGPGT
                     GQYVLAALLIAAAGVDIQKVPYKGAQPVYQDLLSGRIDLFFDNTTTAAPYIEAGQVKP
                     LAVSSRERVTALPRVPTVTETGLLDLEMETWFGLFAPARTPRPAIARLRAEIVRATDQ
                     ADVRKRLEQGSGRSLRMGAAETEAFVRAEVPRWIALVRKAGITPE"
     gene            2175..2771
                     /locus_tag="VI319_06765"
     CDS             2175..2771
                     /locus_tag="VI319_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6863587.1"
                     /translation="MQDWLDQLEIVTLIQNWALWRDTGDWDKLRAAVHPDATMTATWF
                     HGRFDDFIDAAQAAWRERSTSHHALGGTSVELNRSRAIAQTRMTIRVRTQLDGVEVDV
                     SCHGRFFDRVEKRGSAWRIARRSVIYEQDRIDPVEPGTRLVLDRTLLERFPEGYRHLA
                     YVQTKAGAKVDPELPTGVAESQKKMIKEARAWLAAAPR"
     gene            3087..>4626
                     /locus_tag="VI319_06770"
     CDS             3087..>4626
                     /locus_tag="VI319_06770"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY6863588.1"
                     /translation="MAARKTKITSTTAPTPKRAARPIENSSKLPYADLHEHLRTLDKA
                     GLLVTVDRRIDKDTEMHPLVRWQFRGGIEEKDRKAFLFTNVVDAKGKKYDIPVVVGAL
                     AANREIYRLGMGCALESINETWTRAIANPIAPRIVEDAPCHEIVIQGKDLDKPGNALD
                     SIPLPISTPGWDIAPYTTLSQYITKDPDTGVQNMGIYRGMLKAPKRLGMNPSLELRPG
                     IYIHWEKMRKRGQKLPAAVVLGCPPCVAFTSAQKVPETLDELHVAGGLVGAPINVVKA
                     KTVDLLVPAEAEIVVEGYIDTEYLEPEAPFGESHGHVNLQEYNAFMEVTCITRRRDAI
                     LTSIISQVTPSESSLIKRVALEPVFTHHLRNGLGIHGVKKVSMHEPLTNLRKLVVVVM
                     DRNAPRTEVWRALYGAAAWNRAAGKYVVAVNEDIDPDNADAIFWAMSYRANPALDLQL
                     LHHRDQGHGPRSKRNHGEDASVLIDATLKEDFPPISLPKREYMERARKIWEELELPKL
                     KPEAP"
BASE COUNT          744 a         1627 c         1592 g          663 t
ORIGIN      
        1 ccggtttcaa cttctcgccg acgatcggcg tgttcgcgcc cgcgggaacg cacaaggacg
       61 tcatcggcag gatcgcctcg gacgtggccg aggcggtgaa ggtccccgag gtggtcacgc
      121 gcctcacgaa cctcgacatc cagccgatcg gcagcacgcc cgaggaatac gccgccgatc
      181 tgcgggccga cgacgagcgc tattccaagg cggtgaagat ctccggcgcg aaggtcgact
      241 gaggagcgcc gtggccgccg tcgtcaagcc cgaccgcgcg gccttctcgc gcgagctcga
      301 ggcgctcgac gtgcgcccgc tgtgggagcg ggtgatgcgg ctcaagcccg ggaccgcggc
      361 agttccggcg atgtggcgct gggcgcagat gcggccgcgg ctggcgcgcg cctccgagct
      421 gatcaccgcg aaggaagccg agcgccgcgt gctgatgctc gagaacccgg cgctgcgcgg
      481 gatgactttc gccacgacca cgctctacgc ggggctgcag atcatcatgc cgggtgagat
      541 cgccccgacg caccgtcata cgccgaacgc cctgcgcttc atcgtcgagg gcgagggcgc
      601 gtataccgcc gtcgacggcg agcgcgtggc gatgcggccc ggcgacttcg tggtcacgcc
      661 gaactggacc tggcacgatc acggcaacgc cggctccgag ccggtggtgt ggctcgacgg
      721 cctcgacacg gcgttcgcga acctcttcgg cgcgcatttc cgggaggact atcccgagga
      781 gcgccagccg gtcgcacgcg aggacgcggc gtcgccgctg cgttacccgt acgagagcac
      841 gcgcgcggcg ctggcgcggc tcgcgcgaga gggaaatccg gatccggcgc acggccaccg
      901 cttgcgctac cggcgcgccg acaccggcgc cgatccgttt ccgacgatgg cggtattcct
      961 gcagcatctt cccgccgggt tctccgggcg ctcgtaccgc agcaccgacg gagcggtgtt
     1021 ctgcgtgttc gagggccgcg ggaccgtggt catcggggag aagacgttcg agttcgcgcc
     1081 acacgacgtc ttcgtggtgc cgtcctggga ggcgtaccgg ctcgccgccg ccgccgagac
     1141 ggtcctcttc agctattccg accgcgcggc gcaggagacg ctcgggttct ggcgggaatc
     1201 gcgcgagtga gcgccgtgcg cccgctcgcg ttcgccgtcg cgcttctcgc ctgcacgggc
     1261 gtcttcgcgc aagactaccc ggcccgtccc gtgcgcgtcg tcgtgccttc ggcgccgggc
     1321 ggaggcttcg atctcgtcgg gcgcgtcctt tctcagaagc tcggcgagca gaccgggcag
     1381 ccgttcgtgg tcgagaaccg tcccggcgcc ggcacgctgg tcggctcgca gctcgtcgcg
     1441 cgcgcggcgc ccgacggcta cacgctgctg gtcggagggc tttcgaacat cgcgctcaac
     1501 atggcgctgt acaaggcgcc gggctacgac tccctcgccg acctcgcgcc ggtgagcctc
     1561 gtggtgagcc actcgtatac gctcgtcggc cgcaaggacc tgccgcaggg aacgctgcgc
     1621 gaggtgatcg acttcgcccg cgccaatccc ggcaggctca acatggggac gagcggcccc
     1681 ggcaccggcc agtacgtgct ggccgcgctg ctgatcgcgg cggctggcgt cgacatccag
     1741 aaggtgccgt acaagggcgc tcagcccgtg taccaggacc tgctctcggg ccgcatcgat
     1801 ctcttcttcg acaacacgac cacggcggcg ccttacatcg aggcgggaca ggtgaagccg
     1861 ctcgcggtgt cgagccgcga gcgcgtgacc gcgctgcccc gggtgccgac cgtcaccgag
     1921 accgggctgc tcgacctgga gatggagacg tggttcgggc tgttcgcgcc cgcgcgcacg
     1981 ccgcgaccgg cgatcgcgcg gctgcgcgcc gagatcgtgc gcgcgaccga ccaggccgac
     2041 gtgcgcaagc gcctggagca gggtagcggg cgcagcctgc ggatgggcgc ggcggagacc
     2101 gaggcgttcg tgcgcgccga ggtcccgcgc tggatcgctc tcgtgcgcaa ggccggcatc
     2161 acgccggagt gacgatgcag gactggctcg atcagctcga gatcgtcacg ctgatccaga
     2221 actgggcgct gtggcgcgac acgggcgact gggacaagct gcgcgccgcg gtccatcccg
     2281 acgcgaccat gacggcaacc tggttccacg gccgcttcga cgacttcatc gacgccgcgc
     2341 aggcggcgtg gcgagagcgc tcgacctcgc accatgcgct gggcggcacg agcgtcgagc
     2401 tgaaccgctc gcgcgccatc gcccagacgc gcatgacgat ccgcgtgcgg acgcagctcg
     2461 atggcgtcga ggtcgacgtc agctgccacg gccggttctt cgaccgcgtc gagaagcgcg
     2521 ggagcgcctg gcgcatcgcg aggcgcagcg tgatctacga gcaggaccgc atcgaccccg
     2581 tcgagcccgg cacgcggctc gtgctcgacc gaacgctgct cgagcgcttc cccgagggct
     2641 atcgccatct cgcctacgtc cagacgaagg cgggcgcgaa ggtcgacccc gagctgccga
     2701 ccggcgtcgc cgagtcgcag aagaagatga tcaaggaagc gcgcgcctgg ctcgcggccg
     2761 cgccgcggtg agctcgttga aatgagcctt tcgagacggt cgccgcgggg aagcgccgta
     2821 acatcttcgg cttacgctgg cggcatggat catacgctcg cccgccacct gttctggctg
     2881 ttcaccggcg cgctgctcgc cctgtcggca ctctatccgg tatacgtctt gtactgaatc
     2941 gggtctcgcc ggggtcgcgg tccgagcaag gaccggccct cccgaggaga ccggcggccg
     3001 ccgcagttcg ggcggccgta tttttttcgg cgcgccaacg tcccgcattg acacgcgcgc
     3061 gaccgcccct atagcatcgg cggaccatgg ccgcccgaaa aaccaagatc acttccacga
     3121 ccgctccgac gcccaagcgc gcggcgcgcc cgatcgaaaa ttcgtcgaag ctaccgtacg
     3181 ccgacctgca cgagcacctg cgcacgctcg acaaggccgg cctgctcgtc accgtcgacc
     3241 gccgcatcga caaggacacc gagatgcacc cgctggtgcg ctggcagttc cgcggcggca
     3301 tcgaggagaa ggaccgcaag gccttcctgt tcaccaatgt cgtcgacgcg aaagggaaga
     3361 agtacgacat cccggtggtc gtcggcgcgc tcgccgccaa ccgcgagatc taccgcctcg
     3421 gcatgggctg cgcgctcgag agcatcaacg agacctggac gcgcgccatc gccaacccga
     3481 tcgctccgcg gatcgtcgag gatgcgccgt gccacgagat cgtgatccag ggcaaggacc
     3541 tggacaagcc gggcaacgcc ctcgactcga tcccgctgcc gatctcgacg ccgggctggg
     3601 acatcgcgcc gtatacgacg ctgtcgcagt acatcaccaa ggatcccgac acgggcgtgc
     3661 agaacatggg gatctaccgc ggcatgctga aagcgccgaa gcgcctgggc atgaacccgt
     3721 cgctcgagct gcgccccggc atctacatcc actgggagaa gatgcgcaag cgcggccaga
     3781 agctgccggc cgcggtcgtg ctcggctgcc cgccgtgcgt cgcgttcacg tcggcccaga
     3841 aggtgcccga gacgctcgac gagctgcatg tcgccggcgg cctggtcggc gcgccgatca
     3901 acgtcgtgaa ggcgaagacg gtcgacctgc tcgttccggc cgaggccgag atcgtggtcg
     3961 aggggtacat cgacacggaa tacctcgagc ccgaggcgcc gttcggcgag tcgcacgggc
     4021 acgtgaacct gcaggagtac aacgcgttca tggaggtcac ctgcatcacg cgccgccgtg
     4081 acgccatcct gacgtcgatc atctcccagg tgaccccgag cgaatcgagc ctgatcaagc
     4141 gcgtcgcgct cgagccggtg ttcacgcacc acctgcgcaa cggtctcggc atccacggcg
     4201 tgaagaaggt gtcgatgcac gagccgctca cgaacctgcg caagctcgtc gtggtcgtca
     4261 tggaccgcaa cgccccgcgg accgaggtct ggcgcgcgct ctacggcgcg gcggcgtgga
     4321 accgcgccgc cggcaagtac gtggtggccg tgaacgagga catcgatccc gacaacgccg
     4381 acgcgatctt ctgggcgatg tcgtaccgcg cgaaccccgc gctcgacctg cagctgctgc
     4441 accaccgcga ccagggccac ggcccgcgca gcaagcgcaa tcacggcgag gacgcctcgg
     4501 tcctgatcga cgcgacgctg aaggaggatt tcccgccgat ctcgctcccg aagcgcgagt
     4561 acatggagcg cgcgcggaag atctgggagg agctggagct cccgaagctc aagcccgagg
     4621 cgccgt
//
LOCUS       DATDMW010000154         3070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1664664, whole genome shotgun sequence.
ACCESSION   DATDMW010000154 DATDMW010000000
VERSION     DATDMW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664664"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VI319_06775"
     CDS             <1..253
                     /locus_tag="VI319_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6863589.1"
                     /translation="SIETVEGLATQSRPSRLQRAFHEKLGTQCGFCTPGMIMAAEALL
                     RRNPSPSEADIREALSGNICRCTGYVKIIESVQAAAVAP"
     gene            250..2583
                     /gene="hcrA"
                     /locus_tag="VI319_06780"
     CDS             250..2583
                     /gene="hcrA"
                     /locus_tag="VI319_06780"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit alpha"
                     /protein_id="HEY6863590.1"
                     /translation="MTTKTKSAGVPMPLIDGIEKVTGRARYTADLPAHDALVGRILRS
                     PIAHGAIRGIDVSRALALPGVRAVVTGADCDATYGVLPIAMNEYPLARARVRYVGEPV
                     AAVAAVDEATARAAVALIRLDLEPLPAYLTAKDALAEGATLLHPDKPGNVERAVDQEF
                     GDVDGGFRAADLVREASFHYAEVTHAQMEPDCSLAAYDAERDQLTLHSTTQVPYYVHL
                     MLAQCLKMDASRIRVVKPFVGGGFGHRTETLNFELIAALLARAARGTVKLELSREETL
                     ITHRGRPETDVRLKLGLKKNGEITAAECDVVQRGGAYAGYGIVTILYAGALLHAIYRL
                     PASRYHGRRVYANTPPCGAMRGHGCVDVRHAFESLLDRMAGELGLDPFAVRRANLLSA
                     PVRTPNDLQVNSYGLEECLRRVEEASRWRERRGKLGRGRGLGLGCSHYPSGAAKPVHW
                     TGEPHAVVNLKLDFDGAVVALTGASDIGQGSSTLITQVVGEMLGLGPERIRVVANDSA
                     VTPKDNGSYSSRVSFMVGNAAIAAARELRKVLVEAAARRLEVAAADVECLGESYRVVG
                     SDREISYASAVAAALENTGAITVKGTYSTPPETQGGKFRGAAVGTSAGYSYAAQVAEV
                     SVDEDTGEVRVERVWVAHDCGYAINPLSVEGQVQGSVWMGMGQALCEETQYDDASGRP
                     LRANFLDYRILTIADSPPIEVLIVESRDPGGPLGAKEAGEGSLAGFLPALTSAIRDAV
                     GLELDELPASPERVLEALQAKRRAERLKREKSEKAVA"
     gene            2586..>3070
                     /locus_tag="VI319_06785"
     CDS             2586..>3070
                     /locus_tag="VI319_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY6863591.1"
                     /translation="MQILPAFKLIRPASVPEASALLARDPAARVLAGGTDLIANLRHG
                     LGEPSTLVDLGGLVDLAGIEAEGGGLRIGAGVTLAQLAAHARVRAAYAAVAQAAAAIA
                     GPGHRSAATVGGNLCLDTRCVFYNQSDWWRGAKGYCLKHGGDTCHVAPQGKICRAAYS
                     G"
BASE COUNT          453 a         1083 c         1137 g          397 t
ORIGIN      
        1 gagtatagaa accgtcgaag gcttggctac acagagccgt ccgagccggc tgcaacgcgc
       61 ctttcacgaa aagctcggga cgcagtgcgg cttctgcacg ccggggatga tcatggcggc
      121 cgaggcgctg ctgcggcgca atccgtcgcc cagcgaggcg gacatccgcg aagcgctctc
      181 gggaaacatc tgccgctgca cggggtacgt gaagatcatc gagtcggtgc aggcggccgc
      241 ggtggcgcca tgacgacgaa aacgaagagc gccggcgtgc cgatgccgct catcgacggc
      301 atcgagaaag tcaccggccg cgccaggtac accgccgacc tgccggcgca cgacgccctc
      361 gtcggccgca tcctgcggag cccgatcgcg cacggggcaa tccgcggcat cgacgtgtcg
      421 cgcgcgctcg cgctgccggg cgtgcgcgcg gtggtgacgg gcgccgactg cgacgcgacc
      481 tacggcgtgc tgccgatcgc gatgaacgag tacccgctcg cgcgcgctcg cgtgcgctac
      541 gtcggcgagc ccgttgcggc cgtcgctgcc gtcgacgagg cgacggcgcg ggcggcggtt
      601 gcgttgatac ggctcgatct cgagcccctg cccgcgtatc tcacggcgaa ggacgcgctc
      661 gccgaggggg cgacgctgct gcatcccgac aagcccggca acgtcgagcg cgcggtcgac
      721 caggaattcg gcgacgtcga cggaggattc cgcgccgccg acctggtgcg cgaggcgagc
      781 ttccactacg ccgaggtcac gcacgcgcag atggagccgg actgctcgct cgccgcctac
      841 gacgccgagc gcgaccagct cacgctgcat tccacgacgc aggtgcccta ctacgtgcac
      901 ctgatgctcg cgcagtgcct gaagatggac gcgagccgca tccgcgtggt gaagcccttc
      961 gtcggcggcg gcttcggcca ccgcaccgag acgctcaact tcgagctgat cgcggcgttg
     1021 ctcgcgcgcg cggcgcgcgg cacggtgaag ctcgagctct cgcgcgagga gacgctcatc
     1081 acccaccgcg gccggcccga gaccgacgtg cggctcaagc tggggctgaa gaagaacggc
     1141 gagatcaccg ccgccgagtg cgacgtcgtg cagcgcggcg gcgcgtacgc cggctacggc
     1201 atcgtcacca tcctgtacgc cggcgcgctg ctgcacgcga tctaccggct gcccgcgtcg
     1261 cgctaccacg gccggcgcgt gtatgcgaac acgccgccgt gcggcgcgat gcgcggccac
     1321 ggctgcgtcg acgtgcgcca cgcgttcgag tcgctgctcg accgcatggc gggcgagctg
     1381 ggtctcgacc cgttcgcggt gcgccgcgcg aacctcctga gcgcgcccgt gcgcacgccg
     1441 aacgacctgc aggtcaactc gtacggcctc gaggagtgct tgcggcgcgt cgaggaggcg
     1501 tcgcgctggc gcgagcggcg cggcaagctc ggccgcgggc gcggcctggg gctcggctgc
     1561 tcgcactacc cgagcggcgc ggcgaagccc gtgcactgga ccggcgagcc gcacgcggtc
     1621 gtgaacctca agctcgattt cgacggcgcg gtggtcgcgc tgacgggcgc ctccgacatc
     1681 ggccagggct cctcgacgct gatcacgcag gtcgtcggcg agatgctggg gctgggaccc
     1741 gagcgcattc gcgtcgtcgc gaacgacagc gcggtcacgc ccaaggacaa cggctcgtac
     1801 tcctcgcgcg tgtcgttcat ggtcggcaac gccgcgatcg cggcggcgcg cgagctgcgc
     1861 aaggtgctgg tcgaggcggc cgcgcggcgc ctcgaggtcg ctgccgccga cgtcgagtgc
     1921 ctgggcgaga gctaccgcgt cgtgggcagc gaccgcgaga tctcctatgc gagcgcggtc
     1981 gctgcggcgc tcgagaacac gggggcgatc acggtgaaag gcacgtactc gacgccgccc
     2041 gagactcagg gcggcaagtt ccgcggcgcg gccgtgggca cgagcgccgg ctactcgtac
     2101 gccgcgcagg tggccgaggt gagcgtcgac gaggacacgg gcgaggtgcg cgtcgagcgc
     2161 gtctgggtcg cgcacgactg cggctacgcc atcaacccgc tctcggtcga gggccaggtg
     2221 caaggctccg tgtggatggg catgggccag gcgctgtgcg aggagacgca gtacgacgac
     2281 gcgtcggggc ggccgctgcg cgccaacttc ctcgactacc gcatcctcac gatcgccgac
     2341 tcgccgccga tcgaggtgct gatcgtcgag agccgagatc cgggcggtcc gctcggcgcg
     2401 aaggaggcgg gtgaaggctc gctcgccggc ttcctgcccg cgctcacgag cgcgatccgc
     2461 gacgcggtgg ggctcgagct cgacgagctt ccggcgtcgc ccgagcgcgt gctcgaggcg
     2521 ctgcaggcga agcgccgggc cgagcggctg aagcgggaga aatcggaaaa ggcggtggcc
     2581 tgaccatgca gatccttccc gcgttcaagc tgatcaggcc ggcgagcgtg cccgaggcga
     2641 gcgccctgct cgcgcgcgac ccggcggcac gcgtgctcgc cggcggcacc gacctcatcg
     2701 cgaacctgcg ccacgggctc ggcgaaccgt cgacgctggt cgatctcggc gggctcgtgg
     2761 acctcgcggg catcgaggcc gagggcggcg gcctgcgcat cggcgccggc gtgacgctcg
     2821 cacagcttgc cgcgcacgca cgggtgcgcg cggcgtacgc cgcggtcgcg caggccgcgg
     2881 ccgcgatcgc cggtcccggg caccgcagcg ccgccacggt cggcggcaac ctctgcctcg
     2941 acacgcgctg cgtgttctac aaccagagcg actggtggcg cggcgcgaaa ggctattgcc
     3001 tcaagcacgg cggcgacacc tgccacgtgg cgccgcaagg gaagatctgc cgcgccgcgt
     3061 acagcggcga
//
LOCUS       DATDMW010000155         5578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1540064, whole genome shotgun sequence.
ACCESSION   DATDMW010000155 DATDMW010000000
VERSION     DATDMW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540064"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..822
                     /locus_tag="VI319_06790"
     CDS             79..822
                     /locus_tag="VI319_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY6863592.1"
                     /translation="MKTLLILGAAILGTASAHAAELRVLASNGVKAALEELAPAFERE
                     TGHRLALEFGVANVLKRKIDEGAAFDVAILTEANIADLAKAAKIDAASRAGIARSGVG
                     IGVRKGAKHPDISSPEALKRTVLAAKSISWAREGASGQYFAGLVERMGIGEETRRKAV
                     LAANGVEVGKLVGEGKAELAALLVNEIIAAPGMDLVGPLPAALQTYLVFDGGVAGASK
                     SADAAKALIAFLRRPASKAVFTAKGQEPA"
     gene            819..1748
                     /locus_tag="VI319_06795"
     CDS             819..1748
                     /locus_tag="VI319_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6863593.1"
                     /translation="MRASRRTFLRGASALAGACALPAFAQGARTRLVLLGTGGGPRVT
                     AKGRGKPGNLLVIGGVPYVIDCGEGVALALVRAGVALDALRYVFVTHHHSDHNLDYGN
                     LLYDGWAAGLRTPVDAYGPPPIRAITDAYWQMNRFDIETRIADEGRVDLRKLVTVHEF
                     SAPGAVMRNGDVKVTAARVRHPPIEQAYAYRFDGPDRSIAFSGDTAVSPELVALAKGA
                     DVLVSEAMHLGGLESLLKKVPNAATLREHLLASHITTEQLGALAAEAGVKTLVLSHLV
                     PGDDPSITDAMWEEGVRRHYGGRIVVGRDGMEV"
     gene            1748..2623
                     /locus_tag="VI319_06800"
     CDS             1748..2623
                     /locus_tag="VI319_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863594.1"
                     /translation="MEALLYSGAGLGGLIALVGLVLLARRVRGHKAEIDLGGIGRIAT
                     THHGVALVFVGAAMIWQSAAGLAQRDELRATVDRLKRSEEMLVAFVTPSLMRDPSFRS
                     YFAAASPELREAIVRRAMGMLNPEFHAVTDRRQSGFAEEDFATLRTILDFLLADDPHN
                     GSALYYKGEVSRLLRKPFVMREYFGLYLAQAPEPGGRIPGVRGLQYFDERTAWVHHLL
                     ANDYLCEALQTATGKPDLKMVLAEVETVRKYHHEGFTASETLLSTELLEKAALNGAGA
                     VSRPRCAARQGVGKL"
     gene            2703..>5578
                     /locus_tag="VI319_06805"
     CDS             2703..>5578
                     /locus_tag="VI319_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863595.1"
                     /translation="MADSKDTPVPIGGIAVLLLLAVGVFVKHQWPLETSRPGDGQLRS
                     ISPPNVRDLDARLWEDPFAALGRLKKPEDGAPGAAAAARADPDLQSLAARVAPDKQLL
                     VLGVMVYATPYADETELRRRTRYAVLAGLHAGDYSPELYDKIGYFRTGAPPAGAAAWR
                     SAPELVPYELYVTEDRKREVALLWLAEEDFGERMAENLGAFLGELKRAFARHPSVRFG
                     LVGPASSSGLIDITRGCVPQPKPDPALGAPVEVYSANATAVDAAKLPELLGCRVAGAD
                     GRGLTVQMSVVSTITPDARVLEALIDELALRGFAPGRDHVILASEWDTEYGLTLPRQF
                     IAAGCDRATPARRVAGPAIDDAPATAADAGDACRGDATHDPAWVTRLSYLRGLDGDER
                     ASAAKRAAKDKPDGGGADKDAKTREPNDRAIGNGQYDYLRRLADSVEQRARSAPGARE
                     VKAVGVLGSDPYDKLLLLQALRPQFPKAIFFTTDLDARYIHPAEFEWTHNLIVASAHG
                     LELAEAVQGRAPPFRDAYQTSAFLATRLALDRDTPFAKKRERLGHWLASPRVYEIGRS
                     RAYAFQPRGTAPAASGCRDLADCADIRPPLPFFLAPGETRSWALVVAALALIVVSVAA
                     GVWLLRIYLRSFHEATVRVGEALRRPAIGYPALAVVLGVTALALCVPFNSRGEPFTWT
                     AGISIWPSEIIRLAALALCAVFIGRMLRPVALADDEPDGAFFDAGPGASAVAPGTAPA
                     YARVNDGYDEYHDSPGEVDAVTLWRQFRALERPRKRWSRLWPQLVAYAIVFGCLLVGF
                     HDRPNVPARGEFSRGVEHVMSVLSMGAFLAVLLLVIDVIRLGSTFIRNLSTSRSVYPA
                     GTIARVRGELGLGAGHPDHYLCDLLDIRLIAAYTERINAFVYYPFVVLALIVVARSPI
                     FDDWYLPPALALVFLYALVAAVVCAVVLQRAASRAREIAVGRMRG"
BASE COUNT          732 a         2014 c         2060 g          772 t
ORIGIN      
        1 cgatgatgca gtgcggggcg ccgaacgtga aggcgctcaa tccgaagttc gtggggcggt
       61 tctgaccggg agacggacat gaagactttg ctcatcctcg gcgcggcgat cctcggcacg
      121 gcgtcggcgc acgccgccga gctccgcgtc ctcgcctcga acggcgtcaa ggccgcgctc
      181 gaggagctcg cgcccgcgtt cgagcgcgag accgggcacc ggctcgcgct cgagttcggc
      241 gtcgcgaacg tgctgaagcg caagatcgac gaaggcgcgg cgttcgacgt cgcgatcctc
      301 accgaggcca acatcgccga cctggcgaag gccgcgaaga tcgacgccgc ctcgcgcgcg
      361 gggatcgcgc gctcgggcgt cggcatcggc gtgaggaagg gcgcgaagca tcccgacatc
      421 tcgagccccg aggcgctgaa gcgcacggtg ctcgcggcga aatcgatctc gtgggcgagg
      481 gaaggcgcga gcggccagta cttcgccggg ctggtcgagc gcatgggcat cggcgaggag
      541 acgcgcagga aggcggtgct cgccgcgaac ggcgtcgagg tcggcaagct cgtcggcgag
      601 ggcaaggccg agctggcggc gctgctcgtg aacgagatca tcgccgcgcc cggcatggac
      661 ctcgtcgggc cgctgcccgc ggcgctgcag acgtacctgg tgttcgacgg cggcgtggcg
      721 ggcgcgtcga agagcgcgga cgccgcgaag gcgctgatcg cgttcctgag gcggccggcg
      781 tcgaaggccg tgttcaccgc caagggccag gagcccgcat gagagcctcg cgccggacgt
      841 tcctgcgcgg cgcgtccgcg ctcgccgggg cctgcgcgct ccccgccttc gcgcagggcg
      901 cgaggacgcg cctcgtcctc ctcggcaccg gcggcggccc gcgcgtgacg gcgaagggcc
      961 gcggcaagcc gggcaatctc ctcgtgatcg gcggcgtgcc gtacgtgatc gactgcggcg
     1021 agggcgtcgc gctcgcgctg gtgcgcgcgg gcgtcgcgct cgacgcgctg cgctacgtct
     1081 tcgtcacgca ccatcactcc gaccacaatc tcgactacgg caacctgctc tacgacggct
     1141 gggccgcggg gctgcgcacg ccggtcgacg cctacgggcc gccgccgatc cgggcgatca
     1201 ccgacgcgta ctggcagatg aaccgcttcg acatcgagac ccgcatcgcc gacgagggcc
     1261 gcgtcgacct gcgcaagctc gtgaccgtgc acgagttctc ggcgccgggc gcggtgatgc
     1321 ggaacggcga cgtgaaggtc acggccgcgc gcgtgcgcca cccgccgatc gagcaggcgt
     1381 acgcgtaccg gttcgacggc cccgaccgct cgatcgcctt ctcgggcgac accgccgtgt
     1441 cgcccgaact tgtcgcgctc gcgaaaggcg ccgacgtgct ggtgtcggag gcgatgcacc
     1501 tcggcggcct cgagagcctg ctgaagaagg tgccgaacgc ggcgacgctg cgcgagcacc
     1561 tgctcgcgag ccacatcacg accgagcagc tcggcgcgct cgccgcggag gcgggcgtga
     1621 agacgctggt gctgtcgcac ctggtgccgg gcgacgaccc gtcgatcacc gatgcgatgt
     1681 gggaggaagg cgtgcgcagg cactacggcg gccggatcgt cgtcggccgc gacggcatgg
     1741 aggtgtgatg gaggcgctgc tgtactcggg cgccggtctc ggcgggctga tcgcgctcgt
     1801 cggcctggtg ctgctcgcgc gccgcgtgcg cggccacaag gccgagatcg acctcggcgg
     1861 catcggcagg atcgcgacca cgcaccacgg cgtggcgctc gtcttcgtcg gcgcggcgat
     1921 gatctggcag tcggccgcgg gcctcgcgca gcgcgacgag ctgcgcgcga cggtcgaccg
     1981 cctgaagcgc tccgaggaga tgctggtcgc gttcgtcacg ccctcgctca tgcgcgaccc
     2041 gtcgttccgc agctatttcg cggcggcgtc gcccgagctg cgcgaggcga tcgtgcgccg
     2101 cgcgatgggc atgctcaatc ccgagttcca cgccgtcacc gaccgcaggc agtcgggctt
     2161 cgccgaggag gacttcgcga cgctgcgcac gatcctcgac ttcctgctcg ccgacgaccc
     2221 gcacaacggc agcgcgctct actacaaggg cgaggtgagc cggctgctgc gcaagccctt
     2281 cgtgatgcgc gagtacttcg ggctgtacct cgcgcaggcg cccgagcccg gcgggcgcat
     2341 cccgggcgtg cgcgggctgc agtacttcga cgagcgcacc gcgtgggtgc accacctgct
     2401 cgcgaacgac tatctgtgcg aggcgctgca gaccgccacc ggcaagccgg acctgaagat
     2461 ggtcctcgcc gaggtcgaga cggtgcgcaa gtatcaccac gagggcttca ccgcgagcga
     2521 gaccctgctg tcgacggaat tgctcgagaa ggcggcgctg aatggcgccg gcgcggtatc
     2581 gcggccgcgg tgcgcggcgc gccagggcgt gggcaaactg taggggaatt cccgacttcg
     2641 caccgctccc acccgcggat taatatcggt cgcgggctca agaaacccga cagggaggga
     2701 gcttggccga cagcaaggac acgccggttc ccatcggcgg gatcgccgtt ctgcttctgc
     2761 tcgcggtcgg cgtgttcgtc aagcaccagt ggccgctcga gacgtcgcgg cccggcgacg
     2821 ggcagctccg ctcgatcagc ccgccgaacg tgcgcgatct cgacgcccgg ctgtgggagg
     2881 atccgttcgc ggcgctcggg cgcctgaaga agcccgagga cggcgcgccc ggcgccgcgg
     2941 cggccgcgcg cgccgacccg gacctgcagt cgctcgcggc gcgcgtggcg ccggacaagc
     3001 agctgctcgt gctcggcgtc atggtgtacg cgacgccgta cgcggacgag accgagctgc
     3061 gccgccgcac gcgctacgcc gtgctcgccg ggctgcacgc gggcgactac agcccggagc
     3121 tgtacgacaa gatcggctac ttccgcaccg gcgccccgcc ggccggcgcg gccgcgtggc
     3181 ggtcggcgcc cgagctcgtg ccgtacgagc tctatgtcac cgaggaccgc aagcgcgagg
     3241 tcgcgctgct gtggctcgcc gaggaggatt tcggcgagcg catggcggag aatctcggcg
     3301 cctttctcgg cgagctgaag cgcgcgttcg cgcggcaccc ctcggtgcgc ttcggcctgg
     3361 tcgggcccgc gagctcgtcg ggcctcatcg acatcacgcg cggctgcgtg ccgcagccga
     3421 agcccgaccc ggcgctcggc gcgccggtcg aggtctattc ggcgaacgcc acggcggtcg
     3481 acgcggcgaa gctcccggag ctgctcggct gccgcgtcgc cggcgccgac ggccgcgggc
     3541 tcaccgtgca aatgtcggtc gtgtcgacga tcacgcccga tgcacgggtg ctcgaggcgc
     3601 tgatcgacga gctcgcgctg cgcggcttcg cgccggggcg cgatcacgtg atcctcgcct
     3661 cggagtggga caccgagtac ggcctcacgc tgccgcggca gttcatcgcc gcgggctgcg
     3721 atcgggcgac gcccgcgcgc cgcgtggccg gccccgcgat cgacgatgcc ccggcgactg
     3781 cggcggacgc cggcgacgcc tgccgcggcg acgccacgca cgacccggcg tgggtgacgc
     3841 gcctgagcta cctgcgcggg ctcgacggcg acgagcgcgc gtcggccgcg aagcgcgccg
     3901 ccaaggacaa gcccgacggc ggcggcgcgg acaaggacgc gaagacgcgc gagccgaacg
     3961 accgcgcgat cggcaacggg cagtacgact acctgcgccg cctcgccgac tcggtggagc
     4021 agcgcgcgcg ctcggcgccc ggggcgcgcg aggtgaaggc ggtcggcgtg ctcggcagcg
     4081 atccctacga caagctgctc ttgctccagg cgctgcggcc gcagttcccc aaggcgatct
     4141 tcttcaccac cgacctcgac gcgcgctaca tccacccggc ggagttcgag tggacgcaca
     4201 acctgatcgt cgcgtcggcg cacggcctgg agctcgccga ggcggtgcag gggcgcgcgc
     4261 cgccgttccg cgacgcctac cagacctcgg ccttcctcgc gacgcgcctc gcgctcgacc
     4321 gcgacacgcc gttcgcgaag aagcgcgagc ggctcgggca ctggctcgcc tcgccgcgcg
     4381 tctacgagat cgggcgcagc cgcgcgtacg cgttccagcc gcgcggcacg gcgcccgcgg
     4441 cgtcgggctg ccgcgacctc gccgactgcg ccgacatccg cccgccgctg ccgttcttcc
     4501 tcgcgccggg cgagacgcgc agctgggcgc tggtggtggc ggcgctcgcg ctgatcgtcg
     4561 tgtcggtcgc ggccggcgtg tggctgctgc gcatctacct gcgctcgttc cacgaggcga
     4621 ccgtccgcgt gggcgaggcg ctgcggcgcc cggcgatcgg ctacccggcg ctcgcggtcg
     4681 tgctcggggt gaccgcgctc gcgctgtgcg tgccgttcaa ctcgcgcggc gagcccttca
     4741 cctggaccgc gggcatctcg atctggccct cggagatcat ccggctcgcg gcgctcgcgc
     4801 tgtgcgcggt gttcatcggg cggatgctgc ggcccgtggc gctcgcggac gacgagcccg
     4861 acggcgcctt cttcgacgcc gggcccggcg cctcggcggt cgctcccggg accgcgcccg
     4921 cctacgcgcg cgtcaacgac gggtacgacg agtaccacga cagcccgggc gaggtggacg
     4981 cggtgacgct gtggcggcag ttccgcgcgc tcgagcggcc gcgcaagcgc tggagccggc
     5041 tgtggccgca gctcgtcgcc tacgcgatcg tgttcggatg cctgctcgtc ggcttccacg
     5101 accggccgaa cgtgcccgcg cgcggcgagt tcagccgcgg cgtcgagcac gtgatgagcg
     5161 tgctgtcgat gggcgcgttc ctggcggtgc tgctgctcgt gatcgacgtg atccggctcg
     5221 ggtcgacgtt catccgcaac ctgtcgacga gccgcagcgt ctaccccgcc ggcacgatcg
     5281 cgcgggtgcg cggcgagctc ggcctcggcg ccgggcatcc cgaccactac ctgtgcgacc
     5341 tgctcgacat ccgcctgatc gcggcgtaca ccgagcgcat caacgccttc gtctactacc
     5401 cgttcgtggt gctggcgctg atcgtggtcg cgcgcagccc gatcttcgac gactggtacc
     5461 tgccgcccgc gctcgcgctc gtgttcctgt acgcgctggt cgcggcggtc gtctgcgcgg
     5521 tggtgctgca gcgcgccgct tcgcgcgcgc gagagatcgc cgtcggccgc atgcgcgg
//
LOCUS       DATDMW010000156        11064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_916973, whole genome shotgun sequence.
ACCESSION   DATDMW010000156 DATDMW010000000
VERSION     DATDMW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916973"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..603
                     /locus_tag="VI319_06810"
     CDS             223..603
                     /locus_tag="VI319_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863596.1"
                     /translation="MRNKSLLQRFRAGCVIFSTLVLGACATVQGPPPPTPDEIVALAK
                     SGAPAQDIIRRMQESGAVYPLSASQLARLREQGVPDEVIDYMNRTYLDEVRRREAMRQ
                     QFFYGPMFPPYYPYYFGPGLPPWW"
     gene            645..1439
                     /locus_tag="VI319_06815"
     CDS             645..1439
                     /locus_tag="VI319_06815"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY6863597.1"
                     /translation="MAVPPDSLDSAALASRLPTPLSVEVLDECASTSSVLLERAARGA
                     AHGSVVACEHQSAGRGRRGNPWVSARGGSLAFSLLWRFGRPPAALPGLSLAVAVMVAR
                     ALEREGVAGVRLKWPNDLLLGDGKLGGILVELSGDAAGASAAVIGVGLNVHLDAEARA
                     RIGQPAADLAQCAAPLPSRTGLLAALLESLAEGLSRFAREGFAPFRDEWQRRHAWQGR
                     RVALASSGRRVVEGEALGVAEDGALVLRSPDGVERFHSGELSLRPA"
     gene            1444..2157
                     /locus_tag="VI319_06820"
     CDS             1444..2157
                     /locus_tag="VI319_06820"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY6863598.1"
                     /translation="MASLLAIDCGNSVLKWGLHRDGAWERKDAVPLGALVRLEPQWRA
                     LGTVDKVAVSNVAGQAVGLEIERLIARWPVRAIHVAPKPSECGVVNRYEPPERLGADR
                     WAALIGARALERGACLVVDAGTATTADILGADGAFRGGVILPGIDLMKRALAQHAAQL
                     SLAEGQVVAEPRRTADAIETGCVLAQAGAIERLYAGMEPGAACLLSGGNSAKVARALR
                     IETRLVDNLVLEGLLRIAG"
     gene            2154..2969
                     /locus_tag="VI319_06825"
     CDS             2154..2969
                     /locus_tag="VI319_06825"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY6863599.1"
                     /translation="MRIVFFVLLLANLAFFAWTWFTQNASGESQLVDQQLHPEAIKLL
                     TPDQVAKASAKQVPKPAQAAAAKSEPETAPKPQQAPADAAKAPVPAAGKTADVQPAPK
                     APLPVKVAACVELGAFNTAEAARVEQALAPLALGARLSQRRIEETAGFWVYLPPQPNR
                     QGANRKVAELRKLGVGEFFIVQDDAEYRYAISLGVFKSREAARSRLAELRTKGVRTAR
                     IGTRETQVPKVLFSVRDVPEPEAARLNEVRQAFPGAELKDCPLEKPLPPAAPA"
     gene            complement(2966..3451)
                     /locus_tag="VI319_06830"
     CDS             complement(2966..3451)
                     /locus_tag="VI319_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY6863600.1"
                     /translation="MRPDFENKICTPAQLGARMAASARPLVFTNGVFDILHRGHVTYL
                     ARARALGASLLVALNSDASARRLGKGDDRPINPLADRMALVAALEPVTLVTWFEEDTP
                     LALILECRPEVLVKGGDWSPEAIVGASEVRGWGGAVHSVPFEHQRSTTALLRTIRGKA
                     G"
     gene            3516..4073
                     /locus_tag="VI319_06835"
     CDS             3516..4073
                     /locus_tag="VI319_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY6863601.1"
                     /translation="MRLSRLLLFVAAVVPVGASAADACPALLDYTLPSLMDRPTALCQ
                     FRGKVVLIVNTASECGYTPQYQGLEALYRRYRDRGLVVLGVPANDFGGQEPGGNKEIA
                     HFCEVNYGVSFPMFAKSTVAPGKAINPFYEALAARTGQRPQWNFHKYLVDRSGERVLS
                     FASGVEPDDRRLVSELERMLGDGPR"
     gene            complement(4089..4721)
                     /locus_tag="VI319_06840"
     CDS             complement(4089..4721)
                     /locus_tag="VI319_06840"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY6863602.1"
                     /translation="MTRTVLARAVLALGAAAALSGCIPAVILGGAAATAVAISDRRQP
                     EVILGDKRIEYAVSGRIDDALRDQAHINASSYNSTVLLTGEAYTPELKAQAEKIATEV
                     PQVKNVVNEIEVAGRSSLASRSNDSLITTKVKANLISGAGKRYEPEQVKIVTERGVVY
                     LLGLVTREEADAITDVVRSTAGVQKVVRVFEYILPPAKRDSDVRDPTPSR"
     gene            complement(4718..5356)
                     /locus_tag="VI319_06845"
     CDS             complement(4718..5356)
                     /locus_tag="VI319_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEY6863603.1"
                     /translation="MPAMKSFPARGRSAELDLPARIRKHFSDSAELKVRAVDALTAPI
                     AQAVDLMVTALLADRKILSCGNGGSAADAQHFSAELLNRFERERPALAAIALTTDTST
                     LTSIANDYDYDQVFSKQVSALGNAGDVLLAISTSGNSRNVIRAMNVARERDMRIVALT
                     GRGGGEMAKLLGAGDVHVCVPHPQTARIQEVHLLTIHCLCDGIDASLLGAEP"
     gene            complement(5365..5736)
                     /locus_tag="VI319_06850"
     CDS             complement(5365..5736)
                     /locus_tag="VI319_06850"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEY6863604.1"
                     /translation="MRAALLALVAAAACCGDAASAQSARVLVQSSRLAGFVHDEAAAV
                     FPELRVGDALALAREPDNRHDRNAVRVEWRGHKLGYVPRAENEALAWAMDRGETLSAR
                     VTRLRHHPNPRMRIEFDVYME"
     gene            complement(5762..6100)
                     /locus_tag="VI319_06855"
     CDS             complement(5762..6100)
                     /locus_tag="VI319_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY6863605.1"
                     /translation="MKTGAAAEQLAAAWLARKGLQLIEANYRSRFGEIDLVLRDGDTI
                     VFAEVRLRTSDRFGGAAYSVDRRKQSRIIAAARRWLAGKKDAPCRFDVVLMDRLDASR
                     IEWIRDAFTT"
     gene            complement(6097..7095)
                     /locus_tag="VI319_06860"
     CDS             complement(6097..7095)
                     /locus_tag="VI319_06860"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY6863606.1"
                     /translation="MTPAAPVPAPPPSGPYVALLLPLNAPEYAAPAEAVRQGCQAAQT
                     HDPKLALQVVRTNARPDSILAEYNALVARGAAVVVGPMTRTGVSALAARRDPGVPTVA
                     LNTPEREVHAPAWLYSFGLGIESEARLVAHAAMGAKTKDAVVVRANTALAKRASQAFA
                     DEWVLLGGRIAAAEEFGPSTDLPQMQQRLAGVEASMTFLAADATQARTVRPYLNNQMP
                     VYGTSQINDERTGLLVNQDLAGIRFVDMPWLVQPDHPAVMVYPRANSLSVELQRFYAL
                     GIDACRIAAQLVAHAERVRLDGVTGRITLAADHVFQREPVLAVIRDGGASALQDQR"
     gene            7225..8055
                     /gene="rsmI"
                     /locus_tag="VI319_06865"
     CDS             7225..8055
                     /gene="rsmI"
                     /locus_tag="VI319_06865"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY6863607.1"
                     /translation="MVATPIGNLEDIGLRALAVLREVDLVAAEDTRTASILLDHHGIR
                     ARLLALHEHNERKAAESVLAALGEGRDVALVSDAGTPAVSDPGAALVARAREAGFRVS
                     PVPGANAAIAAVSAAGLAQSGFVFEGFLPPKRAARRAAIERLSGERRPIVLYEAPHRV
                     LECVEDLAAVLGGARGLVVARELTKLFEQIHRCRLDEAAAWLQADPHRQKGEFVLIVE
                     GAPAEDPGAAAAAATDPVLRALLAELPLAQAVRLACAITGAKRNAVYARALELSGKKV
                     "
     gene            8075..9112
                     /gene="pyrC"
                     /locus_tag="VI319_06870"
     CDS             8075..9112
                     /gene="pyrC"
                     /locus_tag="VI319_06870"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY6863608.1"
                     /translation="MTATTLTLSRPDDWHLHLRDGPAMAAVLPHAARQFARAIVMPNL
                     RPPVLTTAQAGAYRERILAALPKGAAFTPLMTLYLSDSTGRDEIARAKSSGFVHAVKY
                     YPAGATTNSDYGVTSLERVSGTLEAMQERGLPLLVHGEVTDPEVDVFDRERVFVETVL
                     ERIVRRFPGLRVVLEHVTTREGSDYVSAAGPNVAATITAHHLLYSRGALFAGGIRPHL
                     YCLPVLKREEHRRALVACATGGSRKFFLGTDSAPHARRDKESACGHAGVYTAHAALEL
                     YAEVFEAAGALERLEAFASHHGADFYGLPRNTAKVMLRKEPWVVPRDMPFGSETVVPL
                     RAGESVGWRLS"
     gene            9097..9921
                     /locus_tag="VI319_06875"
     CDS             9097..9921
                     /locus_tag="VI319_06875"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HEY6863609.1"
                     /translation="MAAVLSERRSAALASPIFAPLAPLLARLPRGRFPDEGELNALVP
                     AGTATGAGAALRFVPPSRASPAQYEAQVYETGEVPTRPGEAHDLFNALVWIAFPRTKA
                     VFNRTHYAEMRARRGERLRGTPRDVLTLFDEAGVIVACADRSLAALLAAHEWKALFWS
                     RRADVTRSMRFFVFGHAIYEKALEPYKGVTAKALIVDVEDAFFAQPLERQLARLDERA
                     AAYFADPRSLESTRILPPLPVLGVPGWDPANESAAYYDDAAQFRPLGTAPTGTRAA"
     gene            complement(9899..10411)
                     /locus_tag="VI319_06880"
     CDS             complement(9899..10411)
                     /locus_tag="VI319_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEY6863610.1"
                     /translation="MRRRLYFLLPDLASATQTANDLLLARVDDRCMHFLAKRGTPLGL
                     LREASFLQKSDAIHGAELGMTIGAVGGFLVGVYMFMTPPDGVHMQAVTVLLSTLIGAV
                     FGAWAASLVAMSVPNTRLKAFHADIEGGMILLMVDVPLSRVEEVRELVTRRHPEARSR
                     GVEPTLPAFP"
     gene            10514..10840
                     /gene="rnpB"
                     /locus_tag="VI319_06885"
     ncRNA           10514..10840
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VI319_06885"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1537 a         3922 c         4027 g         1578 t
ORIGIN      
        1 ggcgccgcct ggataaaaaa atacggggga agggagggtt tcccccgtaa gccgccgttt
       61 gctgggccgg cggaaaatcg tgggcgacgc cgcgcggcgc cgcctatgac ggcatcataa
      121 caagcgccga ttggcgcatg caaggccgcc tgaacctaca aaccgcgcaa tgtccaacat
      181 tacagcggcc ttgccgtccg ccgcgacagg ctccgatcac cgatgcgcaa caaatccctg
      241 ctccagcgat tccgcgccgg ctgcgtgatt ttttccacgc tcgttctcgg cgcctgcgcc
      301 acggtgcagg gaccgccgcc cccgaccccg gacgagatcg tggccctcgc gaaatcgggc
      361 gcgcctgcgc aggacatcat ccggcgcatg caggaatccg gcgcggtcta tcccttgagc
      421 gcgtcgcagc tcgcgcggct gcgcgagcag ggcgtgcccg acgaggtgat cgactacatg
      481 aaccgcacgt atctcgacga ggtgcggcgc cgggaggcga tgcggcagca gttcttctac
      541 ggcccgatgt tccctccgta ttacccgtac tacttcgggc cggggctgcc gccgtggtgg
      601 taggcgggat gcccggcgcc gccgtgctac cattcccgcg ccccatggcc gtcccgcccg
      661 attccctcga ctccgcggcg ctcgcgtcgc gcctgccgac gccgctctcg gtcgaggtcc
      721 tcgacgaatg cgcctcgacc agcagcgtgc tgctcgagcg cgcggcgcga ggagccgctc
      781 acggcagcgt cgtcgcgtgc gagcaccaga gcgcggggcg cgggcgccgc ggcaatccgt
      841 gggtctccgc gcgcggcgga agcctcgcgt tctcgctgct gtggcgcttc gggcggccgc
      901 cggccgcgct cccgggcctg agcctcgccg tggccgtcat ggtcgcgcgc gcgctcgagc
      961 gcgaaggggt cgcaggcgtg cgactcaaat ggccgaacga cctcctcctc ggcgacggca
     1021 agctcggggg catcctggtc gagttgtccg gcgacgccgc cggcgcgagc gcggcggtga
     1081 tcggcgtggg acttaacgtg catctcgacg ccgaggcgcg cgcgcgcatc gggcagccgg
     1141 ccgccgatct ggcgcagtgc gcggcgccct tgccgtcgcg caccgggctg ctcgcggcgc
     1201 tgctcgagtc gctcgcggaa gggctctcgc gcttcgcccg cgaaggcttc gctcctttcc
     1261 gcgacgaatg gcagcgccgt cacgcctggc agggccggcg cgtcgcgctc gcgtcctcgg
     1321 gacgccgcgt cgtcgagggc gaagcgctcg gcgtcgccga ggacggcgcg ctcgtgctgc
     1381 gctcgccgga cggcgtcgag cgcttccaca gcggcgagct ctcgctgcgc cccgcctgaa
     1441 agcatggcct cccttctcgc catcgactgc ggcaacagcg tcctcaaatg gggactgcac
     1501 cgcgacggcg cgtgggagcg caaggacgcg gtgccgctcg gcgcgctcgt caggctcgag
     1561 ccgcaatggc gcgcgctcgg gacggtcgac aaggtcgccg tctcgaacgt cgcggggcag
     1621 gcggtcggcc tcgagatcga gcggctgatc gcgcgctggc cggtgcgcgc gatccacgtc
     1681 gcgcccaagc cgagcgagtg tggcgtcgtc aaccgctacg agccgcccga gcgtctcggc
     1741 gccgaccgct gggccgcgct gatcggcgcg cgcgcgctcg agcgcggcgc gtgcctcgtg
     1801 gtcgacgcgg ggacggcgac caccgcggac attctcggcg ccgacggcgc cttccgcggc
     1861 ggcgtcatcc tgcccgggat cgacctgatg aagcgcgccc tcgcccagca cgccgcgcag
     1921 ctctcgctcg cggaaggtca ggtggtcgcc gagccccggc gcaccgccga cgcgatcgag
     1981 accggctgcg tgctcgccca ggcgggcgcg atcgagcgcc tgtacgcggg catggagccc
     2041 ggcgcggcgt gtttgctctc gggcgggaac tcggcgaagg tcgcaagagc gcttcggatc
     2101 gagacccggc tggtggataa tctcgtgctg gagggattgc tgagaatcgc cggatgagga
     2161 tcgtcttctt cgtgctgctg ctcgccaacc tggcgttctt cgcctggacc tggttcaccc
     2221 agaacgcgtc cggcgaatcg cagctcgtcg atcagcagct ccaccccgag gcgatcaagc
     2281 tgctcacgcc ggaccaggtg gcgaaggcgt ccgcgaagca ggtgccgaag ccggcgcagg
     2341 ccgcggcggc gaaatccgag ccggagacgg cgccgaagcc gcagcaggcc cccgcggacg
     2401 cggcaaaggc gcccgtcccg gccgccggca agaccgcgga cgtgcagccc gcgcccaagg
     2461 cgcccttgcc ggtgaaggtg gccgcgtgcg tggagctcgg agccttcaac accgccgagg
     2521 ccgccagggt cgagcaggcg ctggcgccgc tcgcgctcgg cgcgcggctg tcgcagcggc
     2581 gcatcgagga gaccgccggc ttctgggtct acctgccgcc gcagcccaac cggcagggcg
     2641 ccaatcgcaa ggtcgccgag ctgaggaagc tcggcgtggg cgagttcttc atcgtccagg
     2701 acgatgccga gtaccgctac gcgatatcgc tcggcgtctt caagagccgc gaggcggcgc
     2761 gctcgcggct cgccgagctg cgcacgaagg gggtgcgcac cgcccgcatc ggcacgcgcg
     2821 agacgcaggt gccgaaggtc ctgttctcgg tgcgcgacgt gcccgagccc gaggccgcgc
     2881 ggctgaacga agtgcggcag gcttttcccg gggccgagct gaaggattgc ccgttggaaa
     2941 agccgctccc gccggccgcg ccggcctagc ccgcttttcc gcggatcgtc cgcagcagcg
     3001 cggtggtcga gcgctggtgc tcgaacggga ccgagtgcac cgcgccgccc cagccgcgca
     3061 cttccgacgc gccgacgatc gcctcggggc tccagtctcc gcctttgacc agcacctccg
     3121 gccggcattc gaggatgagc gcgagcggcg tgtcctcctc gaaccacgtg accagcgtga
     3181 ccggctcgag cgccgcgacc agcgccatgc ggtcggcgag cgggttgatc gggcggtcgt
     3241 cgcccttgcc gaggcgcctg gccgaggcgt cgctgttcag cgcgaccagc aggctcgcgc
     3301 cgagcgcgcg cgcccgggcg aggtaggtga cgtgaccgcg gtgcaggatg tcgaacacgc
     3361 cgttcgtgaa cacgaggggc cgcgccgacg cggccatgcg cgcgccgagc tgcgccggcg
     3421 tgcagatctt gttctcgaaa tcgggtctca ttttcgctcg cgtcgcgctg cggcgtagaa
     3481 tgcgctcatc cgcgcgagtt taaaggaatc ctgccatgcg actgtcccgg ctgctgctct
     3541 tcgtcgccgc cgtcgtgcct gtgggcgctt cggcggccga tgcctgcccc gcgcttctcg
     3601 attacacgct gcccagcctg atggaccgtc ccaccgcgct gtgccagttc cgcggcaagg
     3661 tggtgctcat tgtcaacacc gcgagcgagt gtggctacac gccgcagtac caggggctcg
     3721 aggccctcta ccgccgctac cgtgaccgcg ggctggtcgt gctcggcgtc cctgcgaacg
     3781 acttcggcgg ccaggagccg ggcggcaaca aggagatcgc gcatttctgc gaggtgaact
     3841 acggcgtgag cttcccgatg ttcgccaagt ccaccgtcgc tccgggcaag gccatcaacc
     3901 ccttctacga ggcgctcgcc gcgaggaccg ggcagcgtcc gcagtggaac ttccacaagt
     3961 accttgtcga ccgcagcggc gagcgcgtgc tctcgttcgc gagcggcgtc gagcccgacg
     4021 accggcgcct cgtgtcggag ctggagcgga tgctcggaga cggtccgcgc tagcggggaa
     4081 gcgtcgcgct agcgcgacgg cgtcgggtcg cgcacgtccg agtcgcgctt cgccggcggc
     4141 aggatgtact cgaagacgcg cacgaccttc tgcacgccgg ccgtgctgcg aacgacgtcg
     4201 gtgatcgcgt cggcctcctc gcgcgtcacc aggccgagca ggtacaccac gccgcgctcg
     4261 gtgacgatct tgacctgctc gggctcgtag cgcttgccgg cgccggagat caggttcgcc
     4321 ttcaccttcg tcgtgatgag gctgtcgttg ctgcgcgagg cgagcgagct gcggccggcg
     4381 acctcgatct cgttcaccac gttcttgacc tgcggcacct cggtggcgat cttctcggcc
     4441 tgcgccttga gctcgggcgt gtaggcctcg ccggtcagca gcaccgtgga gttgtagctg
     4501 ctcgcgttga tatgggcctg atcgcgcagc gcgtcgtcga tgcggccgga gacggcgtac
     4561 tcgatgcgct tgtcgccgag gatcacctcg ggctgccgcc ggtcgcttat cgcgaccgcc
     4621 gtggccgcag cgccgccgag gatcaccgcg gggatgcagc cggagagcgc agcggcggcg
     4681 ccaagggcga ggacggcgcg ggccagaacg gttcgtgtca tggttcagct cccagaagcg
     4741 aggcgtcgat gccgtcgcag aggcagtgga tcgtcaggag gtggacctcc tggatgcgcg
     4801 cggtctgcgg gtgcgggacg cagacgtgga cgtcgccggc gccgagcagc ttcgccatct
     4861 cgccgccgcc gcggccggtg agcgcgacga tgcgcatgtc gcgctcgcgc gcgacgttca
     4921 tcgcgcggat gacgttgcgc gagttgccgc tggtcgagat cgcgagcagc acgtcgcccg
     4981 cattgccgag cgcgctcacc tgcttggaga acacctggtc gtagtcgtag tcgttggcga
     5041 tcgaggtcag cgtggacgta tcggtcgtga gcgcgatcgc ggcgagcgcc ggtcgctcgc
     5101 gctcgaagcg gttgagcagc tccgcggaga aatgctgcgc gtcggccgcc gatccgccgt
     5161 tgccgcacga caggatcttg cggtcggcga ggagcgccgt caccatgagg tccacggcct
     5221 gtgcgatcgg cgcggtgagc gcgtcgaccg cgcgcacctt gagctcggcg ctgtcgctga
     5281 agtgctttcg gatccgtgcg ggcagatcga gctccgcgga ccggccgcgg gcggggaacg
     5341 atttcatggc cggcatttta ccggctactc catgtagacg tcgaactcga tccgcatccg
     5401 gggattggga tggtgtcgca gccgggtgac acgcgccgac agcgtctcgc cgcggtccat
     5461 cgcccacgcc agcgcctcgt tctcggcgcg cggcacgtag ccgagcttgt gtccgcgcca
     5521 ctcgacgcgc accgcgttcc gatcgtggcg attgtcgggc tcgcgcgcga gcgcgagggc
     5581 gtcgccgacg cgcagctcgg gaaacaccgc cgccgcctcg tcgtgcacga agcccgcgag
     5641 acgcgagctc tgcaccagca cgcgggccga ttgcgccgac gccgcgtcgc cgcagcaggc
     5701 cgcggcggcg acgagcgcga gcagggccgc gcgcacgaac cccgtcgcgt atcgtgaacg
     5761 ctcatgtcgt gaacgcatcg cgtatccatt cgatccggga cgcgtcgagc cggtccatga
     5821 gcacgacgtc gaagcgacag ggcgcgtcct tcttgccggc gagccagcgc cgcgcggccg
     5881 cgatgatgcg cgactgcttg cgccggtcca cgctgtaggc ggcgccgccg aagcggtcgc
     5941 tcgtcctcag ccgtacctcg gcaaaaacga tcgtgtcccc gtcccggagg acaaggtcga
     6001 tctcgccgaa gcgcgagcgg tagttcgcct cgatgagctg aaggcccttg cgggcaagcc
     6061 aggccgcggc gagctgctcg gccgcggccc ccgtcttcac cgctggtcct ggagcgccga
     6121 ggcgcccccg tcgcggatca cggcgaggac gggctcgcgc tggaacacgt ggtcggccgc
     6181 gagggtgatg cgcccggtga cgccgtccag ccgcacgcgc tcggcgtggg cgacgagctg
     6241 cgcggcgatc cggcacgcgt cgatcccgag cgcgtagaac cgctgcagct cgaccgagag
     6301 cgaattcgca cgcgggtaaa ccatgaccgc gggatgatcc ggctgcacga gccagggcat
     6361 gtcgacgaag cggatgccgg cgaggtcctg gttgacgagg agcccggtgc gctcgtcgtt
     6421 gatctgcgag gtgccgtaga ccggcatctg gttgttcagg tacggccgca cggtgcgggc
     6481 ctgggtcgcg tcggccgcga ggaaggtcat gctcgcctcg acgccggcga gccgctgctg
     6541 catctgcggc aggtccgtgg aggggccgaa ttcctcggcg gcggcgatgc ggccgccgag
     6601 cagcacccac tcgtcggcga aggcctgcga cgcccgcttc gcgagcgccg tgttcgcgcg
     6661 cacgaccacg gcatccttgg tcttcgcgcc catcgcggcg tgcgcgacca ggcgcgcctc
     6721 gctttcgatg ccgagcccga agctgtagag ccacgccggc gcgtgaacct cgcgctcggg
     6781 cgtgttcagc gcgaccgtcg gcacgccggg gtcgcgccgc gcggcgagcg cggagacgcc
     6841 ggtgcgcgtc atcggcccga ccaccacggc ggccccgcgc gcgaccagcg cgttgtactc
     6901 ggcgaggatc gaatcgggcc gcgcgttggt ccgcacgacc tgcagggcga gcttcgggtc
     6961 gtgcgtctgc gcggcctggc agccctggcg caccgcctcg gcgggcgcgg cgtactcggg
     7021 cgcgttcagc ggcagcagca gcgcgacgta ggggcccgac ggcggcggcg ccgggaccgg
     7081 cgcggcgggc gtcaagggcg cggttgcggg cggctcctga gccgcgcaca atcccgggct
     7141 cgacaacgcg agaacgacga gcagggcttg ggcgcgaaga ccgaggagca tggcggggac
     7201 cgtcggaggg acggccaatt atatgtggtc gcaacgccga ttgggaacct cgaggacatc
     7261 ggcctgcgcg cgctcgccgt gctgcgcgag gtcgacctcg tcgccgccga ggacacgcgc
     7321 accgcgtcga tcctgctcga ccaccacggc atccgcgcgc ggctgctcgc gctgcacgag
     7381 cacaacgagc gcaaggccgc cgagtcggtg ctcgccgcgc tgggcgaagg ccgcgacgtc
     7441 gcgctcgtga gcgacgccgg cacgccggcc gtctccgacc ccggcgccgc cctcgtcgcg
     7501 cgcgcgcgcg aggcgggctt ccgcgtgagc ccggtcccgg gcgcgaacgc ggcgatcgcg
     7561 gcggtctcgg ctgcgggtct cgcgcagagc ggcttcgtgt tcgaaggctt cctgccgccg
     7621 aagcgcgcgg cgcgccgcgc ggcgatcgag cggctgagcg gggagcgccg ccccatcgtg
     7681 ctgtacgagg cgccgcaccg cgtgctcgag tgcgtcgaag atctcgccgc tgtcctcggc
     7741 ggcgctcgcg ggctcgtcgt cgcgcgcgag ctgacgaagc tgttcgagca gatccaccgc
     7801 tgccggctgg acgaggcggc ggcgtggctg caagccgacc cgcaccgcca gaagggcgag
     7861 ttcgtgctca tcgtggaagg cgcgcccgcg gaggacccgg gcgcggcggc cgcagccgcg
     7921 acggacccgg tgctgcgcgc gctgctcgcc gagctgccgc tcgcgcaggc ggtgcggctc
     7981 gcgtgcgcga tcacgggcgc gaagcgcaac gccgtctacg cccgggcgct ggagctctcg
     8041 ggcaagaagg tatgatggac gcggatcgac cgcgatgacc gccaccaccc tcacgctctc
     8101 ccgacccgac gactggcacc tgcacctgcg cgacggaccc gcgatggccg ccgtgctgcc
     8161 gcacgccgcg cgccagttcg cgcgggcgat cgtgatgccg aacctgcgtc cgccggtgct
     8221 gacgaccgcg caggccgggg cgtaccgcga gcgcatcctc gccgcgctgc cgaaaggcgc
     8281 ggcgttcacg ccgctcatga cgctctatct ttccgacagc accggccgcg acgagatcgc
     8341 gcgcgcgaag tcgagcggct tcgtgcacgc ggtcaagtac tacccggcgg gcgcgacgac
     8401 gaactcggat tacggcgtca cctcgctcga gcgcgtctcc gggacgctcg aagccatgca
     8461 ggagcgcggc ctgccgctgc tcgtccacgg cgaggtgacc gatcccgagg tcgacgtctt
     8521 cgaccgcgag cgcgtgttcg tcgagaccgt gctcgagcgc atcgtgcgcc gcttcccggg
     8581 cctgcgcgtg gtgctggagc acgtgaccac gcgcgagggc tcggattacg tctcggcggc
     8641 tgggccgaat gtcgccgcca ccatcaccgc gcaccacctg ctctacagcc gcggcgcgct
     8701 gttcgcgggc gggatccggc cgcatcttta ctgcctgccg gtgctcaagc gcgaggagca
     8761 ccggcgcgcg ctcgtcgcgt gcgcgaccgg cggcagccgc aagttcttcc tcggcaccga
     8821 cagcgcgccg cacgcgcgcc gcgacaagga gagcgcgtgc gggcacgcgg gcgtgtatac
     8881 ggcgcacgcc gcgctcgagc tctacgccga ggtgttcgag gcggcgggcg cgctcgagcg
     8941 cctcgaggcc ttcgcgagcc atcacggcgc cgacttctac gggctgccgc gcaacacggc
     9001 gaaggtgatg ctgcgcaagg agccgtgggt ggtgccgcgc gacatgccgt tcggctccga
     9061 gacggtggtg ccgctgcgcg cgggcgagtc ggtcgggtgg cggctgtcct gagcgaacgc
     9121 cggagcgcgg cgctcgcgtc gccgatcttc gcgccgctcg cgccgctcct cgcgcgactg
     9181 ccgcgcgggc gcttccccga cgaaggcgag ctgaacgcgc tcgtcccggc gggcacggcg
     9241 accggcgcgg gcgcagcgct gcggttcgta ccgccgtcgc gcgcgtcccc ggcgcagtac
     9301 gaggcgcaag tgtacgagac cggcgaggtg ccgacccggc ccggcgaggc gcacgatctc
     9361 ttcaacgcgc tcgtgtggat cgcgtttccg cggaccaagg cggtgttcaa ccgcacgcac
     9421 tacgccgaga tgcgcgcccg gcgcggcgag cggctgcgcg ggacgccgcg cgacgtgctc
     9481 acgctcttcg acgaggccgg ggtgatcgtc gcctgcgccg atcgctcgct cgccgcgctg
     9541 ctcgcggcgc acgagtggaa ggcgctgttc tggagccggc gggccgacgt gacgcgctcg
     9601 atgcgcttct tcgtcttcgg gcacgcgatc tacgagaagg cgctcgagcc ttacaagggc
     9661 gtgacggcga aggcgctgat cgtcgacgtc gaggacgcgt tcttcgcgca gccgctcgag
     9721 cggcagctcg cgcgcctcga cgagcgcgcc gcggcctact tcgccgaccc gcgctcgctc
     9781 gagtcgacgc gcatcctgcc gccactgccc gtgctcggcg tgcccgggtg ggacccggcc
     9841 aacgagagcg ccgcgtacta cgacgacgcg gcgcagttcc gtccgctcgg gaccgcgcct
     9901 acgggaacgc gggcagcgta ggctcgacgc cgcgcgagcg cgcctcggga tgccggcgcg
     9961 tgaccaactc gcggacctcc tcgacgcgcg acagcggcac gtcgaccatg agcaggatca
    10021 tgccgccctc gatgtcggcg tggaaggcct tcagccgcgt gttgggcacc gacatcgcga
    10081 ccaggctcgc ggcccaggcg ccgaacaccg cgccgatcag cgtggagagc aatacggtga
    10141 cggcctgcat gtgcacgccg tcgggcggcg tcatgaacat gtacacgccg acgaggaagc
    10201 cgccgacggc gccgatcgtc atgccgagct ccgcgccgtg gatcgcatcg ctcttctgca
    10261 ggaagctcgc ctcgcgcagg agcccgagcg gcgtgccgcg cttggcgagg aagtgcatgc
    10321 agcggtcgtc gacccgggcg agcagcaggt cgttggccgt ctgggtggcg ctcgccaggt
    10381 cggggagcag gaaatacagt ctgcgtctca tggaggacct ccttgggaga ccaggtgact
    10441 gtgagagtcc attcaagtcc ccgaattccc cgaagtcaag gccgcggccg tcgtcagcgt
    10501 acaatgcggg agtgaagccg gccaggcggt cgctgcgcgt ggtttatccc gcgcagagga
    10561 aagtccgggc tccacagagc ggggtgccgg gtaacgcccg gccgctataa accggaggtt
    10621 tcctccggcg aaggcgagga ccagggccac agagacgagt ctcgtcgggt caaaccggcg
    10681 agggtgaaac gcggcaacct ccacccggag caacaccaaa taggcggacg atgacgctgc
    10741 tcgccgagtc cgcgggtagg tggctagagc cccggggaga cccgcggccc agaggaatga
    10801 ccgtccacga cagaacccgg cttaccggcc ggcttcaccc ttcttcgcgg cggcgcgcag
    10861 cacccatgcc gccatggcgt cgagcatctc gggggcttcg cccagcgccg gcgtgatcgt
    10921 gaactcggtc ttcggatgca gcgagcggat gtcggcgacc atccgcggca ggtcttcctt
    10981 gagatgtccg ccctgtgcga gaaacatcgg cacgatcgtc acccgcgtcg tgccctcgcg
    11041 cgcgagcgct gcgaccgcct cctc
//
LOCUS       DATDMW010000157         4276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2413392, whole genome shotgun sequence.
ACCESSION   DATDMW010000157 DATDMW010000000
VERSION     DATDMW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413392"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VI319_06890"
     CDS             complement(<1..1286)
                     /locus_tag="VI319_06890"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY6863611.1"
                     /translation="MAPEGGAKMKQRVHYRGLRDWLEKVDKMGELARVSGAHWDAEMG
                     ALTHMLTEKSGGTAPALLFDDVPGYPKGFRTLYGHFSSIRRVALTLGLPLEYERKVDI
                     VKQYYERTREMKPIAPRVVKDGPILQNVLEGDACDVLKFPVPRHHEADTSRYIGTADC
                     VITKDPDDGWYNLGAYRSQVYDGRTIGCQITEGKHGRIHRDKYFARGEPMKVVILCGQ
                     DPLLFMLAGLPVPEISELDIAGGLRGEPIDVIRGPYTGFPIPADCEIAIEGETVPGQV
                     RPEGPFGEWMGYYSDDTQPRPYVNVKTILYRNDPILCCAPQHKPVDETGLLKGIGGAA
                     EIWRALEACGIPDVLGVWNHEGAPATRFTVIQIRQRYPGHARNALHVASNCLGGAYAG
                     KWTVVVDEDIDAGDLDQVLWAMSTRFDPASDIDMIQ"
     gene            complement(1296..2252)
                     /locus_tag="VI319_06895"
     CDS             complement(1296..2252)
                     /locus_tag="VI319_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863612.1"
                     /translation="MRRFMVALLVFASVPALAQTYPIRPVKLVVPFAPGGSADLVARI
                     LGQKLGEAWGQQVIVENKGGGSGMIGNDYVAKSAPDGYTLTVGTMGPFSVNPTLFEKM
                     PYDPIRDFAPITLTGIASHVLVANPKVPVHSVSELIALAKAKPGQLTFASSGTGNATH
                     LTFELFKATAGIDIVHVPYKGGGPAMADLVGGQVSFSFASMPSALPYVQSGRLRAIAV
                     GGAHRSPLFPDVPTVAEAGLPGFVSEDWQGILAPAKTPAATVAKLNADLVRTLHTPEM
                     EAKLAGAGFDVKTTTPQEFADFIRADTAKWAKVLRRSGTKAE"
     gene            complement(2249..>4276)
                     /locus_tag="VI319_06900"
     CDS             complement(2249..>4276)
                     /locus_tag="VI319_06900"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6863613.1"
                     /translation="DEVVHVGEPVAIVVADDRYLAEDGANLVEVDYEPLPAVADCQAA
                     LAEGAPKAHRASPHNLLAEFKMGYGDADAAFARAPHVFRESLRQHRGGSHSIECRGCV
                     ASHDAIEDRLTLWSSTQMPHQALRQLCDLLGRDENRVRVVTPDVGGGFGPKLVFYPED
                     AATALAAILVGRPVKWIEDRREHFVATTQERDQVWDVEIAVDRDARILAIRGTIIHDH
                     GAYTARGVNLPHNSAETVPLQYEVPAYAMSVKVALTNKVPVTPVRGAGHPQGTFAIER
                     LLDRVARELKLDRAEVRRRNLIPPEKMPYATKLKARGGQPVLLDSGDYPRCQAEALER
                     AGWSDFPARQRAARAAGRYLGIGLANYCKGTGRGPFESATVRIGPSGKVHVYTGAAAM
                     GQGTRTMLAQVVAEQLGADLANVTVTTGDTAAIALGLGGSNSRQTVTAGSSAHVAAVK
                     VREKTLAIAAQLLEAAAEDLEIAGDHVRVKGVPDMKVSLAKIARSVAGTAGFTLPGGI
                     SPGLEATEHVVIDDMAHANGTAVAEVEVDIETGRVDVLRYVSVHDCGRMVHPTLVDGQ
                     IAGGTAHGIGNALYEWMGYDADAQPVTTTFADYLLVTATEMPRVEIAHQESPSPLNPL
                     GVKGVGECGVVPVTAAIASAVEDALAPFGVRVTQTPMLPAQIAAMIEEARR"
BASE COUNT          563 a         1567 c         1449 g          697 t
ORIGIN      
        1 ttctggatca tgtcgatgtc gctcgccggg tcgaagcgcg tgctcatcgc ccagagcacc
       61 tggtcgaggt cgccggcgtc gatgtcctcg tcgaccacca cggtccactt gcccgcgtac
      121 gcgccgccga ggcagttcga cgcgacgtgc agcgcgttcc gcgcgtggcc cgggtagcgc
      181 tggcggatct ggatcacggt gaagcgcgtc gcgggcgcgc cctcgtggtt ccacacgccg
      241 agcacgtccg ggatgccgca ggcctcgagc gcgcgccaga tctccgcggc gccgccgatg
      301 cccttcagca ggcccgtctc gtcgaccggc ttgtgctgcg gcgcgcagca caggatcggg
      361 tcgttgcggt agaggatcgt cttgacgttc acgtacgggc gcggctgcgt gtcgtccgag
      421 tagtagccca tccactcgcc gaacggcccc tccggccgca cctggcccgg caccgtctcg
      481 ccctcgatcg cgatctcgca gtcggcgggg atcgggaatc ccgtatacgg cccgcggatg
      541 acgtcgatcg gctccccgcg cagtccaccg gcgatgtcga gctcgctgat ctcgggcacg
      601 ggcaggcccg cgagcatgaa cagcagcggg tcctgcccgc acaggatcac gaccttcatc
      661 ggctcgccgc gcgcgaagta cttgtcgcgg tggatgcggc cgtgcttgcc ctccgtgatc
      721 tggcagccga tcgtgcggcc gtcgtagacc tgcgagcggt acgcgccgag gttgtaccag
      781 ccgtcgtcgg ggtccttcgt gatcacgcag tcggcggtgc cgatgtagcg cgaggtgtcg
      841 gcctcgtggt ggcgcggcac cgggaacttg agcacgtcgc aggcgtcgcc ctcgagcacg
      901 ttctgcagga tcgggccgtc cttcacgacg cgcggcgcga tcggcttcat ctcgcgcgtg
      961 cgctcgtagt actgcttgac gatgtcgacc ttgcgctcgt actcgagcgg cagccccagc
     1021 gtcagcgcga cgcgcctgat cgacgagaag tgcccgtaga gcgtgcggaa gcccttcggg
     1081 tatcccggca cgtcgtcgaa gaggagcgcg ggcgccgtgc cgccgctctt ctcggtgagc
     1141 atgtgcgtga gcgcgcccat ctccgcgtcc cagtgcgcgc cgctcacgcg cgcgagctcg
     1201 cccatcttgt ccactttctc cagccagtcg cgcaggcccc ggtagtggac ccgctgcttc
     1261 atcttcgcgc cgccttcggg agccatcttc tcctcctact ccgccttcgt tccagagcgc
     1321 ctcaatacct tcgcccactt cgcggtgtcc gcccggatga agtcagcgaa ctcctgcggc
     1381 gtggtggtct tcacgtcgaa gccggcgccg gcgagcttcg cttccatctc gggcgtatgc
     1441 agcgtgcgca cgagatcggc gttgagcttc gcgacggtcg cggcgggcgt cttcgccggc
     1501 gcgaggattc cctgccagtc ctcggagacg aatccgggaa gcccggcttc ggctaccgtc
     1561 ggtacatcgg gaaacagcgg cgagcgatgc gcgccgccga ccgcgatcgc gcgcagccgc
     1621 ccggactgca cgtaaggcag cgccgagggc atgctcgcga acgagaagct cacctgccct
     1681 ccgacgagat cggccatcgc cggtccgccg cctttgtacg ggacgtgcac gatgtcgatg
     1741 cccgccgtcg ccttgaacaa ctcgaacgtg agatgcgtgg cgttgccggt gccgctcgag
     1801 gcgaaggtca gctgccccgg tttcgccttc gccagcgcga tcagctcgct cacgctatgc
     1861 accggcacct tcggattcgc gaccagcacg tgcgaggcga tacccgtgag cgtgatcggc
     1921 gcgaagtcgc ggatcgggtc gtacggcatc ttctcgaaca gcgtcgggtt caccgagaac
     1981 ggccccatcg tgccgacggt gagcgtgtag ccgtcgggcg cggacttggc gacgtagtcg
     2041 ttgccgatca tgccgctgcc gccgcccttg ttctcgacga tcacctgctg cccccacgcc
     2101 tcgccgagct tctgccccag gatgcgcgcg accagatcgg cgctcccccc cggggcgaac
     2161 ggcacgacca gcttcacggg ccggatcgga tacgtctgcg cgagcgcggg gacggatgcg
     2221 aaaacgagca acgccaccat gaaccgtctc atcgcctcgc ctcctcgatc atcgccgcga
     2281 tctgcgccgg cagcatcggc gtctgcgtga cgcgcacgcc gaagggcgcg agcgcgtcct
     2341 cgaccgccga cgcgatcgcc gccgtgaccg gcaccacgcc gcactcgccc acgcccttca
     2401 ccccgagcgg gttcagcggc gaaggcgact cctggtgcgc gatctcgacg cgcggcatct
     2461 cggtcgcggt gacgagcagg tagtcggcga aggtggtggt cacgggctgc gcgtcggcat
     2521 cgtagcccat ccactcgtag agcgcgttgc cgatgccgtg cgcggtgccg cccgcgatct
     2581 ggccgtcgac cagcgtcggg tgcaccatgc ggccgcagtc gtgcaccgag acgtagcgca
     2641 gcacgtccac gcgcccggtc tcgatgtcca cctcgacctc ggcgaccgcg gtgccgttcg
     2701 cgtgcgccat gtcgtcgatc accacgtgct cggtcgcctc gagccccggg gagattccgc
     2761 ccgggagcgt gaagcccgcc gtgcccgcga cgctgcgcgc gatcttcgcc aggctcacct
     2821 tcatgtccgg gacgcccttg acgcggacgt ggtcgccggc gatctcgagg tcctcggccg
     2881 ccgcctcgag cagctgcgcg gcgatcgcga gcgtcttctc gcgcaccttc accgcggcga
     2941 cgtgcgccga cgaccccgcg gtgacggtct ggcggctgtt cgatccgccc aggccgagcg
     3001 cgatcgcggc ggtgtcgccg gtcgtgaccg tcacgttggc gagatcggcg ccgagctgct
     3061 cggcgactac ctgcgcgagc atcgtcctcg tcccctgccc catcgcggcg gcgcccgtat
     3121 acacgtgcac ctttcccgac gggccgatgc gcacggtcgc cgactcgaac ggcccgcggc
     3181 cggtgccttt gcagtagttc gcgaggccga tcccgagata gcgcccggcc gcgcgcgccg
     3241 ctcgctgccg cgccgggaaa tcgctccagc cggcgcgctc gagcgcctcg gcctggcacc
     3301 tggggtagtc gccgctgtcg agcagcaccg gctggccgcc gcgcgccttg agcttcgtcg
     3361 cgtacggcat cttctccggc gggatcaggt tgcgccttct gacttccgcc cgatcgagct
     3421 tcagctcgcg tgcgacgcga tcgagcagcc gctcgatcgc gaacgtgccc tgcgggtgcc
     3481 ccgccccgcg caccggcgtc accggcacct tgttcgtgag cgcgaccttc acgctcatcg
     3541 cgtacgccgg cacctcgtac tgcagcggca cggtctccgc ggagttgtgc ggcaggttca
     3601 ccccgcgcgc ggtgtacgcg ccgtggtcgt ggatgatcgt cccgcggatc gccaggatgc
     3661 gcgcgtcgcg gtcgaccgcg atctcgacgt cccagacctg gtcgcgctcc tgcgtcgtcg
     3721 cgacgaaatg ctcgcgccgg tcctcgatcc atttcaccgg acggccgacg aggatcgcgg
     3781 cgagcgcggt ggcggcgtcc tcgggataga acacgagctt cggcccgaag ccgccgccga
     3841 cgtctggcgt caccacgcgc acgcggttct cgtcgcgccc gaggagatcg cacagctggc
     3901 gcagcgcctg gtgcggcatc tgcgtcgagc tccatagcgt caggcggtcc tcgatcgcgt
     3961 cgtgcgacgc gacgcagccg cggcactcga tcgagtggct gccgccgcgg tgctggcgca
     4021 gcgactcgcg gaacacgtgc ggcgcgcgcg cgaaggccgc gtccgcgtcg ccgtagccca
     4081 tcttgaactc ggcgagcagg ttgtgcggcg aggcgcggtg cgccttcggc gcgccctccg
     4141 cgagcgccgc ctggcagtcg gccaccgcgg gcagcggctc gtagtcgacc tcgacgagat
     4201 tggcgccgtc ctcggcgagg tagcggtcat cggcgaccac gatcgcgacc ggctcgccga
     4261 cgtgcaccac ctcgtc
//
LOCUS       DATDMW010000158         7916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2163544, whole genome shotgun sequence.
ACCESSION   DATDMW010000158 DATDMW010000000
VERSION     DATDMW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163544"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VI319_06905"
     CDS             complement(<1..882)
                     /locus_tag="VI319_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6863614.1"
                     /translation="MSIDRRSVIKAGLAVGAAGLIAPPILSYGRGETPIKIGMHDPLT
                     GTYAAEGESEVNGAKLALAQVNRKGGMLGRPVELVVEDDAANVGTAVQKAHKLLEKEG
                     CSMLMGAVSSATALAVNQVAHEKGTIYMVTGGHTDPVTGKEAHWTTFRICTTTYMLAA
                     GLVNTLYKKFGKRWYFITPGYAFGHTFQAAYAQILGSLGGTVLGNSLSPLGTTDFSSY
                     LIKAQSTNPEVLILLVDGQDLVNCMKQAVQFGLQKKMAIGGGLSELEVMAALPPEARS
                     GWWTVEWYWNQPKTPHVK"
     gene            1006..1512
                     /locus_tag="VI319_06910"
     CDS             1006..1512
                     /locus_tag="VI319_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY6863615.1"
                     /translation="MRRTKKATPPRPGAGSRHRDTALRVLMQFRQAANSAKRHFRWIG
                     DQCGVSGVHVWALWELSQAPGASVSDVARALAIHQSTASNLLDRLTRDGFLRRERGRA
                     DQRVSRLFVTPAGAALLRRAPSPVRAMLLQALVDLSDADLRQLETLLARLLAHMKVDR
                     SAATRPLD"
     gene            1542..2780
                     /locus_tag="VI319_06915"
     CDS             1542..2780
                     /locus_tag="VI319_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY6863616.1"
                     /translation="MNPNESVELAKHVLLAFGVILLAGTLTALVAQKLRVPDVAMFLL
                     VGTALGPAGAGLLRIDTGSALNQLILIFGSCYILFDGGASLRLKVLKEVWITIVLLAT
                     AGVLITAAVTAAAAWYFLAVPGMVALLLGMAIASTDPATLVPIFRQVRIRDRVAQTVM
                     SESAFNDATGAIGTFAVLGVAMGTGAFSPAHALLDLAQQAGIGIVAGAVLGYAAALLI
                     AHERWAFLAEYAPAVTLMAVIGAYLGADGLQASGFMAVFVFGIVVGNKDAFGFRMSGG
                     EAQKLDEYVATTAFLMRMFIFVLLGSQVDFALMSRYLAGGAAVVAVFMLIARPAAVLL
                     CALPDRRTRWSAREIVFMCWTRETGVIPAALAGLLVGMKAPGAEIIASVTFLAIMITI
                     LVQATTTRWLAGRLGLLLTD"
     gene            complement(2785..3933)
                     /locus_tag="VI319_06920"
     CDS             complement(2785..3933)
                     /locus_tag="VI319_06920"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEY6863617.1"
                     /translation="METAQRSHARLGFAESRPLTIGIELEVQVVNTRDYNLTRGASDL
                     LASLKGGAPYPGEIKPEVTESMIELATSIHAGHAGMLAELVAMRDVTVAAAQRLNVGI
                     AGGGAHPFQTWSDRRIYDTERFNQVAHEYGYLAKQFTVFGQHIHVGCASGDDAVYLTH
                     MLSRYVPHFIALSASSPFHQGEDTLFDSSRLSIVNAFPLSGRIPLVRDWEQFEGYFAK
                     MRGFGIARSMKDFYWDIRPKPEYGTIEIRACDTPLGIAHAAALAAYAQVLARHFLSEP
                     RRPVTDDWYLPYNYNRFQACRYGLEASIIDPVDGRRTVLADDIVAGFGRLEEHAAELR
                     CRPALEALLLGARARENDARRMRAKLRETGSLAGVVRWQCERWMQATI"
     gene            complement(3935..5155)
                     /locus_tag="VI319_06925"
     CDS             complement(3935..5155)
                     /locus_tag="VI319_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY6863618.1"
                     /translation="MSDLSFLPALPFVSTQIAWFGALLLAGIAGGQIAERAFRLPRIT
                     GYAAAGLALGPNGVGAIDIRLLQDLHVLADVSIGLILFDLGQHLDLQWLRRAPRLALT
                     SLAECGCVAVLGYFTLHGFFEIEPLQAATAAAIGVATSPAAVMRITPDLGAQGQVTQR
                     SLLLTALNSSVAVLALTALVPWLYLEYGGALLTILLHPLYLLAGSFLLGLGASAVTLV
                     LARVLRGRDDVQLTLLIGLILVTVGMAIVLKLSVLLALFAYGALLRNLDRNRIVSAVD
                     FGLTGQMFFVILFVLSGANLDLGATWTGIALGASFALARFVGKVAGVMAFIPGSGLKP
                     RQGLCLAASLAPMSGFAVVMLQDTQALFPEFGRSLFTVVLPAVVLMEIFGPIFTQVAL
                     KIAGEARPEPAGRH"
     gene            complement(5322..6725)
                     /locus_tag="VI319_06930"
     CDS             complement(5322..6725)
                     /locus_tag="VI319_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY6863619.1"
                     /translation="MSANLVALWYLLASVCFILALKGLSSPETARRGNAFGIAGMAIA
                     VLTTIAIIAHGRIDLILIAMVIGGSIGALVARRVQMTQMPELVAAMHSLVGMAAVLIA
                     VSVVNNPGAFGEHDPLPRGNRLELFIGTFVGAITFSGSVIAFGKLAGLGKHFRLFSSA
                     PVVFKGQHALNLVLAVVMIGFGVAFFLAEEKAWTPFLVMLAIAFVLGVLIIIPIGGAD
                     MPVVISMLNSYSGWAAAGIGFSLNNTMLIIAGSLVGSSGAILSYIMCKAMNRSFFSVI
                     LGGFGAAEGAAAADSSQKTVKSGSAEDAAFLLANAESVVIVPGYGLAVARAQHAVKEL
                     AQKLIAKGVNVRYAIHPVAGRMPGHMNVLLAEAEVPYDQVVEMEDINGEFGTTDVALV
                     LGANDVVNPLAHEKGSAIYGMPIIEANKARTVLVNKRSMASGYAGLDNPLFYMDKTMM
                     VFGDAKKVVEDMVKALE"
     gene            complement(6722..7045)
                     /locus_tag="VI319_06935"
     CDS             complement(6722..7045)
                     /locus_tag="VI319_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY6863620.1"
                     /translation="MTGTVDPIVVNLIVFVLAIFVGYHVIWNVTPALHTPLMSVTNAI
                     SSVIIVGAILAAGPAQIDLGSVMGAVAVALASVNVAGGFLVTQRMLEMFRKKTPKKTG
                     TAEKK"
     gene            complement(7042..>7916)
                     /locus_tag="VI319_06940"
     CDS             complement(7042..>7916)
                     /locus_tag="VI319_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY6863621.1"
                     /translation="KRGALLVGLLNPYDGAAMQRLAQAGVTAFAMEWLPRTTRAQSMD
                     VLSSQANIAGYKAVIVAANEYQRFMPMLMTAAGTVKAARVLILGAGVAGLQAIATAKR
                     LGAVIEASDVRPAVKEQIESLGAKVLDVPYLTDEEKQIAQGAGGYARPMPADWMRRQA
                     ELVHERAKSADIVITTALIPGRPAPVLIKEDTVRAMKPGAVLLDMAIEQGGNCPLTER
                     DKVVVKHGVKIIGWSNLPAMVAADASALYARNLLNFLALVVDPKTGESKLDRADEIIA
                     GSLVCINGEVVKKS"
BASE COUNT         1189 a         2706 c         2787 g         1234 t
ORIGIN      
        1 cttcacgtgc ggggtcttcg gctggttcca gtaccactcc accgtccacc agcccgagcg
       61 cgcctcgggc ggcagcgccg ccatcacctc gagctccgac aggccgccgc cgatcgccat
      121 cttcttctgc aggccgaact gcaccgcctg cttcatgcag ttcaccaggt cctggccgtc
      181 gacgagcagg atcagcacct cggggttggt cgactgcgcc ttgatcaggt acgacgagaa
      241 gtcggtcgtg ccgagcggcg agagcgagtt gccgagtacc gtgccgccga ggctgcccag
      301 gatctgcgcg tacgcggcct ggaaagtgtg gccgaaggcg tagccggggg tgatgaagta
      361 ccagcgcttg ccgaacttct tgtacagcgt gttcacgagg cccgcggcga gcatgtaggt
      421 cgtcgtgcag atgcggaacg tcgtccagtg cgcttccttg ccggtgacgg ggtccgtgtg
      481 cccgcccgtg accatataga tcgtgccctt ctcgtgcgcg acctggttca ccgcgagcgc
      541 ggtcgccgag gacaccgcgc ccatcagcat cgagcagccc tccttctcca ggagcttgtg
      601 cgccttctgc acggcggtgc cgacgttggc ggcgtcgtcc tcgacgacca gctcgacggg
      661 ccggcccagc atcccgcctt tgcgattgac ctgcgcgagc gcgagcttcg cgccgttcac
      721 ctcgctctcg ccctcggcgg cgtaggtgcc ggtgagcggg tcgtgcatgc cgatcttgat
      781 cggcgtctcg ccgcggccgt agctcaggat cgggggcgcg atcaggcccg cggcgccgac
      841 ggccaggccg gctttgatta cactacggcg atcgatgctc atgtctctcg tctcctcttg
      901 ggttcagccg tggattactt ttgtataaaa catttgtgaa aatgtatctc aaactgtcat
      961 gagacgtcca gcggggcaag gaacgggcgt ccggggaggg ccatgatgcg tcgcaccaag
     1021 aaggccacgc cgccgaggcc cggagcgggc agccgccacc gggacacggc gctgcgggtc
     1081 ctgatgcagt tccggcaggc ggccaactcc gccaagcgcc atttccgctg gatcggagac
     1141 cagtgcgggg tcagcggcgt gcacgtctgg gcgctgtggg agctgagcca ggcgcccggc
     1201 gcgagcgtct cggacgtcgc ccgggcgctc gccatccacc agtcgacggc gagcaacctc
     1261 ctcgacaggc tgacccgcga cgggttcctc cggcgcgagc gcggccgcgc cgatcagcgg
     1321 gtgtcgcggc tgttcgtcac gcccgccggc gcggcgctgc tgcggcgcgc gccctcgccc
     1381 gtgcgcgcga tgctgctcca ggcgctggtc gacttgagcg acgccgacct ccgccagctg
     1441 gagacgctgc tggcgcgcct gctggcgcac atgaaggtcg accggagcgc ggcgacccgg
     1501 cctctcgatt agactggcgg ccggacgaag ggaggacgac gttgaacccg aacgaatcgg
     1561 tcgaactggc caagcacgtg ctgctcgcct tcggcgtgat cctgctcgcg ggcacgctga
     1621 ccgccctggt cgcgcagaag ctgcgcgttc cggacgtggc gatgttcctg ctggtgggga
     1681 ccgcactcgg ccccgccggg gccggcctgc tgcgcatcga caccggctcg gccctgaatc
     1741 agctcatcct gatcttcggc tcgtgctaca tcctgttcga cggcggggcg tcgctgcgcc
     1801 tcaaggtgct gaaggaggtg tggatcacga tcgtcctgct cgcgaccgcc ggcgtgctga
     1861 tcacggcggc cgtcacggcg gccgcggcgt ggtatttcct cgccgtgcct gggatggtcg
     1921 cgctgctgct cgggatggcg atcgcgtcga ccgaccccgc gaccctggtg ccgatcttca
     1981 ggcaggtgcg gatcagggac cgcgtcgcgc agaccgtcat gagcgagtcc gcgttcaacg
     2041 acgccaccgg cgcgatcggc accttcgccg tgctcggcgt ggccatgggc accggcgcct
     2101 tctcgcccgc gcacgcgctg ctcgacctcg cgcagcaggc gggcatcggt atcgtcgcgg
     2161 gcgcggtgct cggctacgcc gcagcgctgc tcatcgcgca cgagcgctgg gccttcctcg
     2221 ccgagtacgc gcccgcggtg accctgatgg cggtgatcgg cgcctacctc ggcgcggacg
     2281 gcctgcaggc gagcggcttc atggcggtgt tcgtgttcgg catcgtggtc ggcaacaagg
     2341 acgcgttcgg gttcaggatg agcggcgggg aggcgcagaa gctcgacgag tacgtcgcga
     2401 cgacggcctt cctcatgcgc atgttcatct tcgtcctgct cgggtcgcag gtcgatttcg
     2461 cgctgatgag ccgctacctc gcggggggcg ccgcggtcgt cgccgtcttc atgctcatcg
     2521 cgcggccggc ggcggtgctc ttgtgcgcgc tgcccgaccg ccgcacgcgc tggagcgcaa
     2581 gagagatcgt cttcatgtgc tggacgcgcg agaccggcgt catccccgcc gcgctcgccg
     2641 ggctcctcgt cgggatgaag gccccgggcg cggaaatcat cgcgtcggtc accttcctcg
     2701 cgatcatgat cacgatcctg gtccaggcga cgaccacccg ctggctcgcg ggccggctcg
     2761 ggctgctgct caccgactga gcacctagat cgtcgcctgc atccagcgct cgcactgcca
     2821 gcgcacgacg ccggcgagcg agccggtctc gcgcagcttc gcgcgcatgc ggcgcgcgtc
     2881 gttctcgcgc gctcgggcgc cgagcagcag cgcctcgagc gcgggccggc agcgcagctc
     2941 ggcggcatgc tcctccagcc gcccgaagcc cgcaacgatg tcgtcggcga gcacggtgcg
     3001 gcggccgtcg accgggtcga tgatgctcgc ctcgaggccg tagcggcacg cctggaagcg
     3061 gttgtagttg tacggcaggt accagtcgtc ggtgaccggg cggcgcggct cgctcaggaa
     3121 atgccgcgcg agcacctgcg cgtaggcggc gagcgcggcg gcgtgcgcga tgccgagcgg
     3181 cgtgtcgcag gcgcggatct cgatcgtgcc gtactcgggc ttggggcgga tgtcccagta
     3241 gaagtccttc atgctgcgcg cgatgccgaa gccgcgcatc ttcgcgaagt agccctcgaa
     3301 ctgctcccag tcgcgcacga gcgggatgcg cccgctcaac gggaaggcgt tcacgatcga
     3361 caggcgcgac gagtcgaaca gcgtgtcctc gccctggtga aacggcgatg aagcggacag
     3421 cgcgatgaag tgcggcacgt agcgcgacag catgtgcgtc aggtagaccg cgtcgtcgcc
     3481 gctcgcgcag ccgacgtgga tgtgctggcc gaaaacggtg aactgcttgg cgagatagcc
     3541 gtactcgtgc gcgacctggt tgaagcgctc cgtgtcgtag atgcgccggt cgctccaggt
     3601 ctggaacggg tgggcgccgc cgcccgcgat gccgacgttg aggcgctgcg ccgcggcgac
     3661 ggtcacgtcg cgcatcgcca ccagctcggc gagcatcccg gcgtgcccgg cgtggatcga
     3721 ggtggcgagc tcgatcatgc tctcggtgac ctcgggcttg atctcgcccg ggtagggcgc
     3781 gccgcctttg agggacgcga gcaggtcgga ggcgccgcgc gtgaggttgt agtcgcgcgt
     3841 gttgacgacc tgcacctcga gctcgatccc gatcgtgagc ggacgcgact cggcgaagcc
     3901 gagacgcgcg tgggaccgct gcgccgtctc cacgtcagtg ccttccggcc ggctccggcc
     3961 gcgcctcgcc cgcgatcttg agagcgacct gggtgaagat aggaccgaag atctccatca
     4021 gcaccaccgc gggcagcacg accgtgaaga ggctccgccc gaactccggg aacagcgcct
     4081 gcgtgtcctg cagcatgacc acggcgaagc ccgacatcgg cgcgaggctc gccgcgaggc
     4141 acagcccctg gcgcggcttc aggccgctgc ccgggatgaa cgccatgacg ccggccacct
     4201 tgccgacgaa gcgcgcgagc gcgaacgacg ccccgagcgc gatgccggtc caggtcgcgc
     4261 ccaggtccag gttcgcgccg ctcagcacga acaggatcac gaagaacatc tggcccgtga
     4321 ggccgaagtc gacggccgag acgatgcggt tgcggtcgag gttccggagc agcgcgccgt
     4381 aggcgaacag cgcgagcagc accgagagct tgagcacgat cgccatcccg acggtcacga
     4441 ggatcagccc gatcagcagc gtgagctgca cgtcgtcgcg cccgcgcagc acgcgcgcga
     4501 gcacgagcgt cacggcgctc gcgccgagcc cgagcaggaa cgagccggcg agcaggtaga
     4561 gcgggtgcag caggatggtg agcagcgcgc cgccgtactc gaggtacagc cacggcacga
     4621 gcgccgtgag cgcgagcacg gcgacgctcg agttgagcgc ggtcaggagc agcgagcgct
     4681 gcgtcacctg cccctgggcg ccgaggtccg gggtgatgcg catcaccgcc gcgggcgagg
     4741 tggcgacgcc gatcgcggcg gcggtcgcgg cctgcagcgg ctcgatctcg aagaacccgt
     4801 gcagcgtgaa gtagccgagc accgcgacgc agccgcactc ggcgagcgag gtcagcgcga
     4861 gccgcggcgc gcgccgcagc cactgcaggt cgaggtgctg gccgaggtcg aacaggatca
     4921 ggccgatcga gacgtctgcg agcacgtgca ggtcctgcag caggcggatg tcgatcgcgc
     4981 cgacgccgtt cgggcccagc gcgaggcccg ccgccgcgta gccggtgatg cgcggcagcc
     5041 ggaacgcgcg ctcggcgatc tgtccgccgg cgatgccggc gagcagcagc gcgccgaacc
     5101 acgcgatctg ggtggacacg aacggcagtg ccggcaggaa cgacagatcc gacatgcgct
     5161 ggacctctgc tctgtttcgc cgcggcggga aggcgtgtgc agctcgcttg ttgttcgccg
     5221 gcccgggcgc cggcttacat tcttttcgag aagacccggc ttcgacaagc gcggcggcgt
     5281 cgggttccgc cggaggatca gcgggttgtg ggtttacccg cctattcgag cgccttgacc
     5341 atgtcctcga cgaccttctt cgcgtcgccg aagaccatca tggtcttgtc catgtagaag
     5401 agcggattgt cgaggccggc gtagcccgag gccatggagc gcttgttcac gagcacggtg
     5461 cgcgccttgt tcgcctcgat gatcggcatg ccgtagatcg cgctgccttt ctcgtgcgcg
     5521 agcgggttca cgacgtcgtt cgcgccgagc acgagcgcga cgtcggtagt gccgaactcg
     5581 ccgttgatgt cctccatctc gaccacctgg tcgtagggca cctcggcctc ggcgagcagc
     5641 acgttcatgt ggccgggcat ccgtcccgcg accgggtgga tggcgtagcg gacgttcacg
     5701 cccttggcga tcagcttctg cgcgagctcc ttcaccgcgt gctgggcgcg cgccaccgcc
     5761 aggccgtagc cgggcacgat caccaccgat tccgcgttcg cgagcaggaa ggccgcgtcc
     5821 tcggccgagc ccgacttcac cgtcttctgc gagctgtccg cggccgccgc gccctcggcc
     5881 gcgccgaacc cgcccaggat cacgctgaaa aaggaccggt tcatcgcctt gcacatgatg
     5941 taggagagga tcgcgcccga ggagccgacg agcgacccgg cgatgatcag catcgtgttg
     6001 ttgagcgaga agccgatgcc cgccgcggcc cagcccgagt agctgttcag catcgagatc
     6061 acgaccggca tgtcggcgcc gccgatcgga atgatgatca gcacgccgag cacgaacgcg
     6121 atcgcgagca tgaccaggaa cggcgtccac gccttctcct cggcgaggaa gaaggcgacg
     6181 ccgaagccga tcatcacgac cgcgagcacg aggttcagcg cgtgctgccc cttgaacacg
     6241 accggcgcgc tcgagaacag ccggaagtgc ttgccgaggc cggcgagctt gccgaaggcg
     6301 atgacgctgc ccgagaacgt gatcgcgccg acgaaggtgc cgatgaagag ctcgaggcgg
     6361 ttgccgcgcg gcagcgggtc gtgttcgccg aaggcgccgg ggttgttgac gaccgagacc
     6421 gcgatcagca ccgcggccat gccgaccagc gagtgcatcg ccgcgaccag ctcgggcatc
     6481 tgcgtcatct gcacgcgccg ggccacgagc gcgccgatcg agcctccgat caccatcgcg
     6541 atgaggatca ggtcgatgcg gccgtgcgcg atgatcgcga tcgtggtcag gaccgcgatc
     6601 gccatcccgg cgatgccgaa ggcgttgccg cgccgcgccg tctccggcga cgacagccct
     6661 ttcagcgcca ggatgaagca gaccgacgcg agcaggtacc agagcgcgac gaggttggcg
     6721 ctcatttctt ctccgcggtc ccggtctttt tcggggtctt tttccggaac atctcgagca
     6781 tgcgctgcgt cacgaggaag ccgcccgcga cgttcaccga ggcgagcgcg accgcgaccg
     6841 cgcccatgac ggagccgagg tcgatctgcg ccggccccgc ggccaggatc gcgccgacga
     6901 tgatcaccga ggagatggcg ttggtcacgc tcatcagcgg ggtgtgcagc gccggcgtca
     6961 cgttccagat gacgtgatag ccgacgaaga tcgcgagcac gaacacgatg aggttgacga
     7021 cgatggggtc gacggtgccg gtcatgattt cttcaccacc tcgccgttga tgcacaccag
     7081 gctgcccgcg atgatctcgt ccgcgcggtc gagcttcgat tcgcccgtct tcgggtcgac
     7141 gacgagcgcg aggaagttga gcaggttgcg cgcgtagagc gcgctcgcgt cggcggcgac
     7201 catcgccggc aggttcgacc agccgatgat cttcacgccg tgcttcacca ccaccttgtc
     7261 gcgctcggtg agcgggcagt tgccgccctg ctcgatcgcc atgtcgagga gcacggcccc
     7321 gggcttcatc gcgcgcacgg tgtcctcctt gatcagcacc ggcgccgggc gccccgggat
     7381 cagcgccgtc gtgatgacga tgtcggcgct cttcgcgcgc tcgtgcacga gctcggcctg
     7441 ccggcgcatc cagtcggcgg gcatcggccg cgcgtagccg cccgcgccct gcgcgatctg
     7501 cttttcctcg tcggtgagat agggcacgtc gaggaccttg gcgccgagcg actcgatctg
     7561 ctccttcacc gccgggcgca cgtcggaggc ctcgatcacc gcgccgaggc gcttggccgt
     7621 cgcgatcgcc tgcaggccgg cgacgcccgc gccgaggatc agcacgcgcg cggccttcac
     7681 cgtgcccgcg gcggtcatca gcatcggcat gaagcgctgg tactcgtttg cggcgacgat
     7741 gacggccttg tagccggcga tgttcgcctg cgaggagagc acgtccatcg actgcgcgcg
     7801 cgtcgtgcgc ggcagccatt ccatggcgaa cgcggtgacg cccgcctgcg cgaggcgctg
     7861 catggcggcg ccgtcgtagg ggttgagcag gccgaccagg agggcgccgc gcttca
//
LOCUS       DATDMW010000159         5638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2288147, whole genome shotgun sequence.
ACCESSION   DATDMW010000159 DATDMW010000000
VERSION     DATDMW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288147"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..661)
                     /gene="pal"
                     /locus_tag="VI319_06945"
     CDS             complement(131..661)
                     /gene="pal"
                     /locus_tag="VI319_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY6863622.1"
                     /translation="MKIVSIRTALVLLVLGFVSGCASQASHDQAGATPASGAGSAAVQ
                     PSAGAAASSSISGSADTAASERAAAERSVFYGFDQYAVDSQERSIVEANARYLRAHPQ
                     TKVRIEGNADERGSREYNLALGQRRADAVMKMMNVLGVRESQMETVSYGKEKPRAAGH
                     DEDAWKQNRRSDIVYR"
     gene            complement(825..1727)
                     /locus_tag="VI319_06950"
     CDS             complement(825..1727)
                     /locus_tag="VI319_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6863623.1"
                     /translation="MKLALKVDVDTFRGTREGVPRLIEILKRHGARATFLFSLGPDHT
                     GRAAKRVFRKGFMHKVSRTSVREHYGTKTLLYGTLLPGPDIGKRAAPAMHQARDAGFE
                     VGIHCWDHVRWQDHVVGADGVWTEREMRRSVERFRQVFGTAPTAHGAAGWQMNVHALR
                     LTQRLGFAYSSDTRGTSPFIPVCRAEVVLCPQIPTTLPTLDEMIGLDGTTADNVHEKL
                     LESTATPAQHVYTLHAELEGGKLAPAFERLLEGWKAQGYELVAVREVADGLDLKKLPR
                     HEMVMDQIPGRSGEVAVQGPEFLA"
     gene            complement(1737..2780)
                     /locus_tag="VI319_06955"
     CDS             complement(1737..2780)
                     /locus_tag="VI319_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEY6863624.1"
                     /translation="MKKILILGVNGFIGHHLSKRIVAATDWEVYGMDMQTDRISDLLD
                     EPRFHFFEGDITINREWIEYHVRKCDTVLPLVAIATPATYVKQPLRVFELDFEANLPI
                     VRSCVKHGKRLVFPSTSEVYGMCRDREFDPEASELVLGPINKPRWIYSCAKQLMDRVI
                     HAYGMEQGLDYTLFRPFNWIGAGLDNLNAPKEGSSRVITQFLGHIVRGEPIKLVDGGK
                     QKRAFTYVDDGIDALLRIIENPRRIASGKIYNVGNPRNNYSVRELAQMMVKLALKYDE
                     YRPNARKVRLVKTTAAQYYGKGYQDVQNRVPRIANTMKELGWKPRTNMQQALEHIFEA
                     YREHVAEAQRLVE"
     gene            complement(2777..3697)
                     /locus_tag="VI319_06960"
     CDS             complement(2777..3697)
                     /locus_tag="VI319_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEY6863625.1"
                     /translation="MTRAVVFAYHDVGARCLEVLLRHGVQVPLVVTHEDDPKEAIWFE
                     RVADRAARHGIPAITPGDPNAAATVARVRKAKPDFLFSFYYRRMLGPKLLALPTRGAF
                     NMHGSLLPKYRGRAPVNWAILKGETETGATLHEMVAKPDAGRIVGQERVAIDEDDLAV
                     DVFRKMTAAAEAVLERSLPKLVDGTAELREQDLAAGSYFGGRTPEDGRIDWSRPAREV
                     HDLVRAVAPPFPGAFTELGGERVRVLRTRLDPARQPRAGGVGLYRDALQWFADCADGK
                     VLRLLEVEAGADLERRAAAGKKIPLPHKKP"
     gene            complement(3704..4693)
                     /locus_tag="VI319_06965"
     CDS             complement(3704..4693)
                     /locus_tag="VI319_06965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6863626.1"
                     /translation="MSPRLSVVIPVYNEEQVLPALFERLYAALDALALPYEVIFVDDG
                     SRDKSAALLRAQFQKRPDATRVVIFNANFGQHMAIMAGFERCRGERVVTLDADLQNPP
                     EEIGKLLAKMDEGHDYVGGVRAERHDTAFRRYASRLMNAFRERITQIRMTDQGCMLRA
                     YSRDIIAAINSCREVNTFIPALAYSFARKPAEVEVAHEPRAAGDSKYSLYKLVQLNFD
                     LVTSFSVKPLHFFSMLGMLVAALSVMAYVIVIIQRWLEAGSFADGVRAVWDRDILEFF
                     LIGLMLFGLGLLGEYIGRIYELVRQRPRYIVQAVLEKIDERPDSTQAKQSIGR"
     gene            complement(4690..>5638)
                     /locus_tag="VI319_06970"
     CDS             complement(4690..>5638)
                     /locus_tag="VI319_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEY6863627.1"
                     /translation="IGLKLAGVGAGDEVITTPLSWVATANVIVQAGARPVFVDVDPAT
                     RNIDLAKMQAAIGPRTKALLPVYLAGLPVDLDRLYAVAASGKLRVLEDAAQAFGSSWR
                     GTAIGARGDLVAFSFHANKNITTAEGGAIVLPSETDVPLCEKLRLQGVVRSGEDGMEV
                     DVAGGKFNLTDVAARIGLGQLPRLAEFTAKRRALAKRYFARFDRSLGCGLPPEDFENS
                     NWHMFQVVLPPDRLPRAEFIARMREAGIGVGVHYPAIHLFALYRKLGYREGQFPNAER
                     IGRAIATLPLFPAMEPSDVDRVCDAAAKILSQVRGGAHA"
BASE COUNT          818 a         1913 c         1983 g          924 t
ORIGIN      
        1 aaggccgccg ccgcggcgac caggacggcg agcagcgccg gtatcacccg gcgccgcgag
       61 ctgcttgccc tgtcgagata gacggggcga tcttccgcgg cggaccagtg ctccatgact
      121 gcctctgact tcagcgatag acgatgtcgc tgcggcggtt ctgcttccag gcgtcctcgt
      181 cgtggccggc ggcgcgcggc ttctccttgc cgtagctcac ggtctccatc tgcgactcgc
      241 gcacgccgag gacgttcatc atcttcatca cggcgtcggc gcggcgctgg ccgagagcga
      301 ggttgtactc gcggctgccg cgctcgtcgg cgttgccctc gatgcggacc ttcgtctgcg
      361 ggtgcgcgcg caggtagcgc gcgttggcct cgacgatgct tcgttcctgg gagtcgaccg
      421 cgtactggtc gaagccgtag aacacgctgc gctcggcggc ggcgcgctcg ctcgccgcgg
      481 tgtccgcgga cccggagatg ctcgaggacg cggccgcgcc cgccgacggc tggacggccg
      541 cgctgccggc gccggaggcc ggcgtcgcgc cggcctggtc gtgcgacgcc tggctggcgc
      601 agcccgatac gaatccgagg acaagcagga ccagagcagt gcggatcgag acgatcttca
      661 tggtgtgttc tcccgtggtg tagtccaacc ggattgttgg acaggatcgc tcacgagtgt
      721 gctacagatt gttacgccgc tctagttccg ctgaaccacg ccgatggcgc ccgcgcggcg
      781 ctgcatgggg taaaccccga tgcgatcgcg ccaatggcgc acggctacgc gagaaactcg
      841 ggtccctgca ccgcgacctc gcccgagcgc cccgggatct ggtccatcac catctcgtgt
      901 cgcggaagct tcttgagatc gagcccgtcg gcgacctcgc gcacggcgac cagctcgtag
      961 ccctgggcct tccagccctc gagcaggcgc tcgaacgccg gcgcgagctt gccgccttcg
     1021 agctcggcgt ggagcgtgta cacgtgctgg gcgggcgtcg cggtgctctc gagaagcttc
     1081 tcgtgcacgt tgtcggccgt cgtcccgtcg agcccgatca tctcgtcgag cgtcggcagc
     1141 gtcgtcggga tctgcgggca cagcacgacc tcggcgcggc acacggggat gaagggcgag
     1201 gtcccgcgcg tgtcggacga gtaggcgaag ccgaggcgct gcgtgaggcg cagcgcgtgc
     1261 acgttcatct gccacccggc cgcgccgtgc gcggtcggcg cggtgccgaa cacctgccgg
     1321 aagcgctcga ccgacctgcg catctcgcgc tcggtccaga cgccgtcggc gcccaccacg
     1381 tggtcctgcc atcgcacgtg gtcccagcag tggatgccga cctcgaagcc ggcgtcgcgc
     1441 gcctggtgca tggcgggcgc ggcgcgcttg ccgatgtccg gaccggggag cagcgtgccg
     1501 tagagcaggg tcttcgtgcc gtagtgctcg cgcacggacg tgcgcgacac cttgtgcatg
     1561 aagcccttgc ggaagacgcg cttcgcggcg cggccggtgt gatcggggcc gagcgagaac
     1621 aggaacgtgg cgcgcgcgcc gtggcgcttg aggatctcga tcaggcgcgg gacgccctcc
     1681 cgcgtgccgc ggaaggtgtc gacgtcgacc ttgagcgcga gcttcattcg accgcgctac
     1741 tcgaccagcc gctgcgcctc ggcgacgtgc tcgcggtacg cctcgaagat gtgctcgagc
     1801 gcctgctgca tgttcgtgcg cggcttccag ccgagctcct tcatcgtgtt cgcgatccgg
     1861 ggaacgcggt tctggacgtc ctggtagcct ttcccgtagt actgcgccgc ggtcgtcttc
     1921 acgagccgga ccttcctcgc gttggggcgg tattcgtcgt acttcaacgc gagcttcacc
     1981 atcatctgcg ccagctcgcg caccgagtag ttgttcctcg ggttgccgac gttgtagatc
     2041 ttgccgctcg cgatccgccg cgggttctcg atgatcctca gcagcgcgtc gatgccgtcg
     2101 tcgacgtagg tgaaggcgcg tttctgcttc ccgccgtcga ccagcttgat cggctcgccg
     2161 cgcacgatgt ggccgaggaa ctgcgtgatc acgcgcgagc tgccttcctt cggcgcgttg
     2221 agattgtcga ggcccgcgcc gatccagttg aacgggcgga agagggtgta gtcgagcccc
     2281 tgctccatgc cgtaggcgtg gatcacgcgg tccatcagct gcttcgcgca ggaatagatc
     2341 cagcgcggct tgttgatcgg gccgagcacg agctccgacg cctcggggtc gaactcgcgg
     2401 tcgcggcaca tgccgtagac ctccgaggtc gacgggaaca cgaggcgctt cccgtgcttc
     2461 acgcacgagc gcacgatcgg caggttcgcc tcgaagtcga gctcgaacac gcgcagcggc
     2521 tgcttcacgt aggtcgcagg cgtggcgatg gcgaccagcg gcagcaccgt gtcgcacttc
     2581 cggacgtggt actcgatcca ctcgcggttg atcgtgatgt cgccctcgaa gaaatggaag
     2641 cgcggctcgt cgagcaggtc ggagatgcgg tcggtctgca tgtccatgcc gtagacctcc
     2701 cagtccgtcg cggcgacgat gcgcttcgac aggtggtggc cgatgaagcc gttgacgccg
     2761 aggatcagga tcttcttcat ggttttttgt gcgggagcgg gattttcttg ccggccgcgg
     2821 ctcggcgctc gagatcggcg ccggcctcga cctcgagcag acgcagcact ttaccatcgg
     2881 cgcagtcggc gaaccattgc agcgcgtcgc ggtacaggcc gacgccgccg gcgcgcggct
     2941 ggcgcgcggg atcgagccgg gtgcgcagca cgcgcacgcg ctcgccgccg agctcggtga
     3001 acgcgccggg gaagggcggc gcgaccgcac gcaccaggtc gtgcacctcg cgcgcggggc
     3061 ggctccagtc gatgcggccg tcctcgggcg tgcggccgcc gaaatagctg ccggccgcga
     3121 ggtcctgctc gcgcagctcg gcggtgccgt cgacgagctt cggcaggctg cgctcgagca
     3181 ccgcctcggc cgccgcggtc atcttgcgga agacgtcgac cgcgaggtcg tcctcgtcga
     3241 tcgccacgcg ctcctggccg acgatgcggc cggcgtcggg cttcgcgacc atctcgtgca
     3301 gcgtcgcgcc ggtctcggtc tcgcccttga ggatcgccca gttgaccggc gcgcggccgc
     3361 ggtacttcgg caggagcgac ccgtgcatgt tgaacgcgcc gcgcgtgggg agggcgagca
     3421 gcttcggacc gagcatcctg cggtagtaga acgagaacag gaaatcgggc ttcgccttcc
     3481 tcactcttgc gaccgtcgcc gcggcgttcg ggtccccggg cgtgatcgcg gggatgccgt
     3541 ggcgcgccgc gcgatcggcg acgcgctcga accagatcgc ttccttcggg tcgtcctcgt
     3601 gcgtcacgac cagcggcacc tgcacgccgt ggcgcagcag cacctcgagg cagcgcgcgc
     3661 cgacgtcgtg gtaggcgaac acgaccgcgc gcgtcaccgt ccctcagcgc ccaatggact
     3721 gcttcgcttg cgtcgaatca ggccgctcgt cgattttttc gagcaccgcc tggacgatgt
     3781 agcgcgggcg ctgccgcacc agctcgtaga tgcggccgat gtactcgccg agcagcccca
     3841 gcccgaacag catcaggccg atgaggaaga actccaggat gtcgcggtcc cagaccgcgc
     3901 gcacgccgtc ggcgaaggag ccggcttcca gccagcgctg gatgatgacg atcacgtagg
     3961 ccatcaccga gagcgcggcg accagcatcc cgagcatcga gaagaagtgc agcggcttca
     4021 ccgagaagct ggtcaccagg tcgaaattga gctgcaccag cttgtacagc gagtacttgg
     4081 agtcgccggc cgcgcgcggc tcgtgcgcga cctcgacttc ggcgggcttg cgcgcgaagc
     4141 tgtacgcgag cgcggggatg aaggtattga cctcgcggca gctgttgatc gcggcgatga
     4201 tgtcgcggct gtaggcgcgc agcatgcagc cctggtcggt catgcggatc tgcgtgatgc
     4261 gctcgcggaa ggcgttcatc aggcgcgagg cgtagcgccg gaaggcggtg tcgtggcgct
     4321 cggcgcgcac gccgccgacg tagtcgtggc cctcgtccat cttcgcgagc agcttgccga
     4381 tctcctcggg cgggttctgc aggtcggcgt cgagcgtcac cacgcgctcg ccgcggcagc
     4441 gctcgaagcc cgccatgatc gccatgtgct ggccgaagtt ggcgttgaag atcaccacgc
     4501 gcgtcgcgtc gggccgcttc tggaactgcg cgcgcagcag cgccgcggac ttgtcgcggc
     4561 tgccgtcgtc gacgaagatc acctcgtagg gcaacgcgag cgcgtcgagc gcggcgtaca
     4621 gccgctcgaa cagcgcgggg agcacctgct cctcgttgta gacggggatg acgaccgaca
     4681 gccgagggct catgcgtggg cgcctcccct gacctgagac aggatcttgg cggcggcgtc
     4741 gcagacgcgg tcgacgtccg agggctccat cgcgggaaag agcggcagcg tcgcgatggc
     4801 gcggccgatg cgctcggcgt tcggaaactg cccttcgcga tatccgagct tgcgatagag
     4861 cgcgaacagg tggatcgccg ggtagtgcac gccgacgccg atgccggcct cgcgcatgcg
     4921 cgcgatgaac tcggcgcgcg gtaagcgatc aggtggcagc accacttgga acatgtgcca
     4981 gttggaattc tcgaaatcct cgggcggcag gccgcagccg agcgagcggt cgaagcgagc
     5041 gaagtagcgc ttcgcgagcg cgcgccgctt cgcggtgaac tcggcgagcc gcggaagctg
     5101 gccgaggccg atgcgcgccg cgacgtcggt gaggttgaac ttgccgcccg cgacgtcgac
     5161 ctccattccg tcctcgcccg agcgcaccac gccctggagc ctcagcttct cgcacagcgg
     5221 gacatccgtc tcggacggca gcacgatggc gccgccctcc gcggtcgtga tgttcttgtt
     5281 ggcgtggaac gagaacgcga ccaggtcccc gcgcgcgccg atcgcggtgc cgcgccagct
     5341 cgagccgaag gcctgcgccg cgtcttccag gacgcggagc ttgccgctcg cggcgaccgc
     5401 atacaggcgg tcgagatcga ccggcagtcc cgcgagatag accggcagca gcgccttcgt
     5461 tcgcggcccg atcgccgcct gcatcttcgc gagatcgatg ttgcgcgtgg ccgggtcgac
     5521 gtcgacgaac accggccgcg cgcccgcctg cacgatgacg ttcgcggtcg cgacccacga
     5581 cagcggcgtc gtgatcacct cgtcgcccgc gccgacgccg gcgagcttca gcccgatc
//
LOCUS       DATDMW010000160         5777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2038415, whole genome shotgun sequence.
ACCESSION   DATDMW010000160 DATDMW010000000
VERSION     DATDMW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038415"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /gene="mutL"
                     /locus_tag="VI319_06975"
     CDS             complement(<1..771)
                     /gene="mutL"
                     /locus_tag="VI319_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY6863628.1"
                     /translation="MPPIRLLPDVLVSQIAAGEVVERPASVLKELLENSLDAGASEVS
                     VALVEGGIRRVRVSDDGCGIDASDLPMAFARHATSKIASLEELEAVRSLGFRGEALAS
                     IASVARVTLTSRTDGAATAFALSAEGGSSQPARPAAHARGTTVEVEDLYFNTPARRKF
                     LRSDATELAHCQDVFRRAALSRPDVAFTLRHNERVVARMAPAQLSARVGAVLGDDFAA
                     SMRALGETGPGLSVLGYVGSPDAARGSRDAQFTFVNGRF"
     gene            823..1473
                     /locus_tag="VI319_06980"
     CDS             823..1473
                     /locus_tag="VI319_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY6863629.1"
                     /translation="MEILLGAWDFVVHLDKHLAALLQQHGAWVYVLLFLIIFGETGLV
                     VTPFLPGDSLLFVAGTLAATGGIDVHLLAVVLMLAAVLGNTANYSIGYWLGPKVFHWE
                     QSRWFNRRALDRAHAFYERHGGKTIVVTRFVPILRTFAPFVAGIARMTYVRFTAYNVT
                     GAVLWVGLLLYAGYWFGNVPMVRDNLAIVMLLIVAVSLAPIAVEIVRHRARSAGGG"
     gene            complement(1470..2780)
                     /locus_tag="VI319_06985"
     CDS             complement(1470..2780)
                     /locus_tag="VI319_06985"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY6863630.1"
                     /translation="MPRRAAQRLIGAVRVAAVVLAALLGSPQLVRADTIEPIRAVRIW
                     PAADYTRLTLETAQPLRYNLLSLKNPDRLVLDLEDVEPERVQELLAGKILPIDPYIAA
                     ARVGRFKPGVARLVLDLKEAVKPQIFVLAPVGDYGHRLVLDLYPAVPIDPLMALLQKE
                     QLDPAAAGPVDPGATKNLGEPKDAGKPRGVPRPAVARLITVAVDAGHGGEDPGAKGRR
                     GTYEKNVTLLIARKLKAVIDAEPNMRAFLTRDGDYYIALGERVGKARRVKADLFVSVH
                     ADAFVKPHARGSSVFALSERGATSTAAQWLAKRENDADLIGGVNLNRKDRYLAMTLAD
                     LSLTAQISDSLKLGNAVLSELGGINSLHRGRVEQAGFAVLKAPDVPSILVETAFISNP
                     DEERRLANAAYQEKIANAIFRGIKGYFAKNPPLSRSTLAANGSL"
     gene            complement(2753..3244)
                     /gene="tsaE"
                     /locus_tag="VI319_06990"
     CDS             complement(2753..3244)
                     /gene="tsaE"
                     /locus_tag="VI319_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY6863631.1"
                     /translation="MTHPCNHSQPHSLELALSDEAATRRAGARLARCVAPGLKIYLRG
                     PLGAGKTTFVRGLLGGLGWRGKVKSPSYALVEVYVVSSIHLYHFDFYRFRDPGEWDDS
                     GFRETFDSPAVCIVEWPEKAGGALPPADLEIGLELALPGRALRASAVGPAGERCLGAL
                     RNG"
     gene            3236..4372
                     /gene="queG"
                     /locus_tag="VI319_06995"
     CDS             3236..4372
                     /gene="queG"
                     /locus_tag="VI319_06995"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY6863632.1"
                     /translation="MSHAARDRVGSVDFEMLAAAVKGWGRELGFQAIGISDVDLSEAE
                     PRFAEWLARGYAGELDYMRKHGAKRTRPGELVPGTLRVISARMDYRPQAADAGAVLAD
                     GAKAFIARYALGRDYHKTLRGRLQRLAERVEGEVGAFGYRVFTDSAPVLEVELARKAG
                     LGWRGKNTLLLAREAGSFFFLGEIYTDLPLPVDVPAGEHCGTCERCLDACPTRAIVAP
                     YVLDARRCISYLTIELKGSIPVELRPLVGNRVYGCDDCQTVCPWNRYAQVSPEPDFRV
                     RHGLDGAELIELFGWTEDEFRERTAGSAIARIGYERWCRNLAVGLGNAPTSAAVVAAL
                     ERRSADPSPLVREHVAWALARHVGPAAGPNEGVGDAVRRARNAL"
     gene            4482..>5777
                     /locus_tag="VI319_07000"
     CDS             4482..>5777
                     /locus_tag="VI319_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6863633.1"
                     /translation="MSSFAELGLLPELLKAVSEQGYTEPTPIQAQAIPYVLQGRDLMG
                     GAQTGTGKTAGFTLPLLQRLARHQNTSASPARHPVRALILTPTRELAAQVEESVRTYG
                     KYLSLRCTVVFGGVPMDPQTAALRRGVEFLVATPGRLLDHVQQKTVNLQQVEAFVLDE
                     ADRMLDMGFIPDVRRIMALMPKQRQNLLFSATLPAEIKTLADSFLHDPVTVQVARRNA
                     TADLVTHVVHPVRRERKRELLAYLIQSRELRQVLVFAATRIGANRLAYQLNKDGIHTA
                     AIHGDKSQPERLQALADFKSGKVGVLVATDVAARGLDIEDLPHVVNFELPNSPEDYVH
                     RIGRTGRAGASGEALSLVCAEEHERLAAIEKTIKLSISKVIVPGFEPDARIVPTLIAG
                     GRGREGREGRERRPRGDRDRLTVAAAKPSRPGKPVDPIFD"
BASE COUNT          852 a         2008 c         2030 g          887 t
ORIGIN      
        1 gaagcggccg ttgacgaagg tgaactgcgc atcgcgcgac ccgcgcgcgg cgtcgggcga
       61 gccgacgtag ccgagcaccg agaggccggg accggtctcg ccgagcgcac gcatcgaagc
      121 cgcgaaatcg tcgccgagca ccgcgccgac gcgcgcgctc agctgcgcgg gcgccatgcg
      181 cgcgacgacc cgctcgttgt ggcgaagcgt gaacgcgaca tccggacgcg acagcgcggc
      241 gcgccggaac acgtcctggc agtgcgcgag ctcggtcgcg tcagagcgca ggaatttgcg
      301 gcgcgcgggc gtgttgaagt aaaggtcctc gacctccacc gtggtgccgc gcgcgtgcgc
      361 cgcgggccgc gccggctgcg acgagccgcc ctcggccgat agcgcgaatg cggtcgcggc
      421 gccgtcggtg cggctcgtga gcgtcacgcg cgcgaccgaa gcgatgctgg cgagcgcctc
      481 gccgcggaac ccgagcgagc gcaccgcctc gagctcctcg agcgaggcga tcttgctggt
      541 ggcgtggcgc gcgaaggcca tgggcaggtc ggaggcgtcg atgccgcagc cgtcgtcgct
      601 gacccgcacg cgccggattc cgccttcgac gagcgcgacc gacacctcgc tcgccccggc
      661 gtcgaggctg ttctcgagca gctccttcag gaccgacgcc gggcgctcga cgacttcgcc
      721 ggccgcgatc tggctgacga ggacgtcggg caggagtcgg atagggggca tctgcgctgc
      781 caagctcgcg gccattatag gtgatcgcgc tagaatgcct cgatggaaat cctgctcggc
      841 gcatgggact tcgtcgttca cctcgacaag catcttgccg cgctgctgca gcagcacggc
      901 gcctgggtct acgtgctgtt gttcctgatc atcttcggcg agaccgggct ggtcgtcacc
      961 cctttccttc cgggcgactc gctgctcttc gtcgcgggga cgctcgcggc gaccggaggc
     1021 atcgacgtgc acctgctcgc ggtcgtcctc atgctcgcgg cggtcctggg caacaccgcc
     1081 aattattcga tcgggtactg gctcggaccg aaggtgttcc actgggagca gtcgcgctgg
     1141 ttcaaccggc gcgcgctcga tcgcgcgcac gccttctacg aacggcacgg cggaaagacg
     1201 atcgtcgtga cacgcttcgt tcccatcctg cgcaccttcg cgcccttcgt cgccgggatc
     1261 gcgcggatga cttacgtgcg gttcaccgcg tacaacgtca ccggcgccgt gctgtgggtc
     1321 ggactgctgc tctacgcggg ctactggttc ggcaacgtgc cgatggtgcg cgacaacctg
     1381 gcgatcgtga tgctgctgat cgtcgcggtg tcgctcgcgc cgatcgcggt cgagatcgtg
     1441 cgccaccgcg cgcgcagcgc gggcggcggc tagagcgagc cgttcgccgc gagcgtcgag
     1501 cgcgacagcg gcgggttctt cgcgaagtag cccttgatgc cgcggaagat cgcgttcgcg
     1561 atcttctcct ggtaggccgc gttcgcgaga cgccgctcct cgtcggggtt gctgatgaac
     1621 gcggtctcga cgaggatgga aggcacgtcg ggcgctttca gcacggcgaa ccccgcctgc
     1681 tcgacccgtc cccggtgcag cgaattgatg ccgcccagct cgctcagcac ggcgttcccg
     1741 agcttgaggc tgtcgctgat ctgcgcggtc aggctcagat cggcgagcgt cattgccagg
     1801 taccggtcct tgcggttgag gttcacgccg ccgatcaggt cagcgtcgtt ctcgcgcttc
     1861 gccagccact gcgccgcggt gctggtcgcg ccgcgctcgg acagcgcgaa caccgacgag
     1921 ccgcgcgcat gcggcttgac gaaagcgtcg gcgtgcaccg acacgaacag gtcggccttg
     1981 acccggcgcg ccttcccgac gcgctcgccg agcgcgatgt agtaatcgcc gtcgcgggtc
     2041 aggaacgcgc gcatgttggg ctcggcgtcg atgacggcct tcagcttgcg cgcgatgagc
     2101 agcgtcacgt tcttctcgta cgtcccgcga cgtcccttcg cgccggggtc ctcgccgccg
     2161 tgcccggcgt cgaccgcgac cgtgatcagg cgcgcgaccg ccggcctggg cacgccgcgc
     2221 ggcttgcccg cgtccttggg ctcgcccagg ttcttcgtgg cgccgggatc gacaggaccc
     2281 gcggccgccg ggtcgagctg ctccttctgc agcagcgcca tcaacggatc gatgggcacg
     2341 gcggggtaga ggtcgagcac gaggcggtgg ccgtaatcgc cgacaggcgc cagcacgaag
     2401 atctggggct tgaccgcctc cttgagatcg aggaccagcc gcgcgacgcc cggcttgaat
     2461 cggccgacgc gcgccgcggc gatgtacgga tcgatgggca ggatctttcc agcgagcagc
     2521 tcctggacgc gctcgggctc cacgtcctcg agatcgagga ccaggcggtc gggattcttg
     2581 aggctgagca ggttgtagcg cagcggctgc gcggtctcga gcgtgagccg ggtgtaatcg
     2641 gccgccggcc agatgcgaac cgcccggatg ggctcgatgg tgtcggcacg aacgagttgc
     2701 ggggagccga gcagcgcggc gagcacgacg gcggcgaccc gcaccgcgcc gatcagccgt
     2761 tgcgcagcgc gcctaggcat cgttccccgg ccgggccgac ggcgctcgcc cgcagcgcgc
     2821 ggcccgggag cgccagctcc aagccgatct ccaggtcggc tgggggaagc gcacccccgg
     2881 ccttttcggg ccattcgacg atgcagaccg cgggactgtc gaaggtttct cgaaagccgc
     2941 tgtcatccca ttctcctgga tcccggaagc gataaaaatc aaagtggtac aagtgtatgc
     3001 tagaaacgac ataaacttca accagcgcat agctcggact cttcactttg ccgcgccagc
     3061 cgagcccgcc gagcagcccc cggacgaacg tcgtcttccc ggcgccgagc ggcccgcgca
     3121 ggtagatctt cagccccggc gcgacacagc gcgcgaggcg cgccccggcg cgccgggtcg
     3181 ccgcttcgtc ggaaagagcg agctccagcg agtgtggctg gctatgatta cagggatgag
     3241 tcacgcagcg cgggaccggg tcggttcggt cgatttcgag atgctggcgg ctgccgtcaa
     3301 gggatggggc cgggagctcg gctttcaggc gatcggcatt tccgacgtcg atctttccga
     3361 ggcggagccg cgcttcgcgg aatggctggc gcgcggatac gcgggcgagc tggattatat
     3421 gcgaaagcac ggggcgaaac ggacccggcc cggcgagctc gtccccggca cgctgcgcgt
     3481 gatctccgcg agaatggatt accggccgca ggcggccgat gccggcgcgg tgctcgccga
     3541 cggggcgaag gcgttcatcg ctcgctacgc gctcggccgg gactaccaca agacgctgcg
     3601 cggccggctg cagcggctcg ccgagcgcgt cgaaggcgag gtcggcgcct tcggctaccg
     3661 tgtgttcacc gactcggcgc cggtgctcga agtcgagctc gcgcgcaagg cgggcctcgg
     3721 ctggcgaggc aagaacacgc tcctgctcgc gcgcgaggcg ggctcgttct tctttctcgg
     3781 cgagatctac accgacctgc cgctcccggt cgacgtgccc gccggggagc actgcgggac
     3841 ctgcgagcgc tgcctcgacg cctgtccgac gcgggccatc gtggcgcctt acgtgctcga
     3901 tgcgcgccgc tgcatctcct acctgacgat cgagctgaag ggcagcatcc cggtggagct
     3961 gcggcccctc gtcggcaacc gcgtgtacgg ctgcgacgac tgccagaccg tgtgcccctg
     4021 gaaccgctac gcccaggtat cgccggagcc cgacttccgc gtgcgccacg ggctggacgg
     4081 cgccgagttg atcgagctct tcggatggac ggaggacgag ttccgcgagc ggacggcggg
     4141 cagcgcgatc gcgcgcatcg gctacgagcg ctggtgccgc aacctcgcgg tcggcctggg
     4201 caacgcgccg accagcgccg cggtggtcgc cgccctcgag cgccgttcgg ccgatccgtc
     4261 gccgctcgtg cgcgagcacg tggcctgggc gctcgcgcgc catgtcggac cggccgctgg
     4321 tccgaatgaa ggggttggcg atgcagtccg ccgcgcccgg aacgcgctat aatctgcagg
     4381 ttgtcatacc cacttttcgt ccgccgccct cgctcatccg gccttcgcgc cgcgcctgaa
     4441 gcgtctccgg cctgcaaccc gggccgaagg agattccccg tttgtccagc ttcgccgaac
     4501 taggactgct cccagagctg ctgaaagcgg tctctgagca aggctatacc gagccgacgc
     4561 cgatccaggc gcaggcgatt ccgtatgtgc tgcaggggcg cgacctgatg ggcggcgccc
     4621 agaccggcac cggcaaaacc gccggcttca cgctgccgct gctgcagcgg ctggcgcgcc
     4681 accagaacac cagcgcgtcg cccgcgcgcc acccggtgcg tgcgctcatc ctgacgccta
     4741 cgcgcgagct cgccgcgcag gtcgaggaga gtgttcggac ctacggcaag tacctgagcc
     4801 tgcgctgcac cgtggtcttc ggcggcgtgc caatggaccc gcagacggcg gcgctgcgcc
     4861 ggggcgtcga gttcctggtc gcgacgccgg ggcggctgct cgatcacgtg cagcagaaga
     4921 ccgtaaacct gcagcaggtc gaggccttcg ttctggacga ggccgaccgc atgctcgaca
     4981 tgggcttcat cccggacgtg cgtcgcatca tggcgctgat gccgaagcag cggcagaacc
     5041 tgctgttctc cgccacgctg ccggcggaga tcaagaccct cgccgattcc ttcctgcacg
     5101 atcccgtgac ggtgcaggtc gcccgccgca acgcgaccgc ggacctcgtg acgcacgtgg
     5161 tgcacccggt gcgccgggag cgcaagcgcg agctgctcgc ctacctcatc cagtcgcggg
     5221 agctgcgcca ggtgctcgtg ttcgccgcga cccgcatcgg cgcgaaccgg ctcgcctacc
     5281 agctcaacaa ggatggcatc cacaccgccg cgatccacgg cgacaagagc cagcccgagc
     5341 ggctgcaggc cctggcggat ttcaagtcgg gcaaggtggg cgtgctggtg gcgaccgacg
     5401 tcgcggcgcg cgggctggac atcgaggacc tgcctcatgt cgtcaacttc gagctgccga
     5461 actcgcccga ggactacgtg caccgtatcg gccgcaccgg ccgcgccggc gcgagcggcg
     5521 aggcgctgtc cctcgtatgc gccgaggagc acgagcggct cgcggcgatc gagaagacga
     5581 tcaagctttc gatctccaag gtcattgtcc cgggattcga gcccgacgcg cgcatcgtgc
     5641 cgacgttgat cgctggcggg cgcggccgcg aagggcgcga aggccgcgag cggcggccgc
     5701 gcggcgaccg cgaccggctc accgtggccg cggccaagcc cagcaggccg ggcaagcccg
     5761 tggacccgat cttcgac
//
LOCUS       DATDMW010000161         5515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1915491, whole genome shotgun sequence.
ACCESSION   DATDMW010000161 DATDMW010000000
VERSION     DATDMW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915491"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VI319_07005"
     CDS             <1..810
                     /locus_tag="VI319_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY6863634.1"
                     /translation="VDVVVTSIGDARSLLGGTLLLTPLKGADAVVHALAQGPLSVGGY
                     KYDLNGNVVQKNHPTVGAIPSGATVELAVATEVVKDDHLSFVLSDPDYTTASRVADSL
                     NTTLGSGAVARAVDAGRIEIAIPPEERARLVTYMTRIENVSIEPDQRAKVVVNERTGT
                     VVSGGDVRISHVVVSHGDLKVSITTDYLVSQPTGVAFTGPGVRTAVVPRTRIEVQEAP
                     ADGNVAVVEVSNTVADLVKALARIKTQPRDMISILQGIKAAGALHADLIIQ"
     gene            830..1198
                     /locus_tag="VI319_07010"
     CDS             830..1198
                     /locus_tag="VI319_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863635.1"
                     /translation="MDTGALTGATGALIRMALDGVSMRQLATANNIANASTSDYAPVH
                     VDFEQQLADAMRDAARGRGPDPARLLPTVTTDPTPGAKVQLDLEATQMAQDVLHYQAL
                     LRALREQMDVLQSAINDGRK"
     gene            1198..1638
                     /gene="flgC"
                     /locus_tag="VI319_07015"
     CDS             1198..1638
                     /gene="flgC"
                     /locus_tag="VI319_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY6863636.1"
                     /translation="MDYLAAFAISGSGMTVEKMRLDVTAMNLANVNSSRGADGTMFQP
                     LRVVSGVRTDPRFEAAMAAWGVQLGGAQVQQLVPTDAPPRMVLEPGHPDADSRGFVSY
                     PGINPVAEMTNLITAVRTYEANVVALNAAKTMAQRALDIGGGGR"
     gene            1635..1964
                     /gene="fliE"
                     /locus_tag="VI319_07020"
     CDS             1635..1964
                     /gene="fliE"
                     /locus_tag="VI319_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY6863637.1"
                     /translation="MSIDAVGFIPSATAQAIESKLPGSAVGTGTGTGAAPASGFGDWL
                     ASEIAQVNDKIQSADKKVQDLALGKASNLHDVMIALEDTKLSFQLMVQIRSRVLEAYQ
                     DILRMQV"
     gene            1974..3461
                     /gene="fliF"
                     /locus_tag="VI319_07025"
     CDS             1974..3461
                     /gene="fliF"
                     /locus_tag="VI319_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY6863638.1"
                     /translation="MANARERWAALGAGAKVSIVTGLLVVVVLLIAGAAWILRDDYQV
                     LFADLDPQDGAAIVAELDRMKVGYRLADSGRTILVERDAVHKTRLKIMGGKGVPLKGN
                     VGFEIFNNSDFGMTEFAQKINYQRALQGELARTIMSIEEIRQARVHLAFPEGGLLKKN
                     SAQPKASVTLALRGDSRLRPEQIVGIQRLVAAAVPDMDPQAVTVLDHQGVALSRLADE
                     RDDMGGVGGRLELKKSTEVYLTKKAIEVLDRAFGPGQAIVSIDVTLSQDSVKTTREDV
                     IPFARKDGEFAGAVTRRRTNVQGVATPRGYEVQATADLAKQTPQPVGTQTSSVEVEYE
                     NGKQVEQVVTTPGSIKRISVGVVLPGRMDAQRIQRVSDVVAMAVGLQRQRGDASAVHA
                     VDELGARSVSGLPAALPEPAPSEEPPQARAVARDAGPKSVDLSVPIAAALAMLLAVAL
                     ALFVAASRRRPEPPTMTAEDREKALSQIRAWVQGGEPTPTQETRS"
     gene            3458..4039
                     /locus_tag="VI319_07030"
     CDS             3458..4039
                     /locus_tag="VI319_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863639.1"
                     /translation="MSDGLRLAALRLHGVAAADRDWILDRLGRRDRRALGLLLRDLKR
                     SGIVAAHVEAAKEELAAAPERERLEPVAPGVPADLAHADPEAVARALSGEPEWVRSTI
                     LAMHHWKWRRAVLQRLAAGAPDSSVRSAPVAAAPHPAFAAALARSLARRIGETSAELE
                     TRGASFERVMDNLDPLARSPRPRIWERLRAWMR"
     gene            4027..4785
                     /locus_tag="VI319_07035"
     CDS             4027..4785
                     /locus_tag="VI319_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEY6863640.1"
                     /translation="MDALIRGAHVSERAVVLGVRRPGHAEAEPVARPAPAGTGADPCP
                     VGARVESAAPEAAWLESERRKIADELRLELDARLEREAEEARKAGHAEGVEQGRAEAL
                     REASEQAAAGTRALAAVAKHAEREIEGAQDLLVGIAFEAVCKLLGARALEREAVAGMV
                     HEVIARVKRDEAMLVRLHPQDCALLRGAAGDPRGDGDRHFKVELVADDKVALGGCLVE
                     TEGGLLDARLETQLERLRATLLDARASTRPAEHP"
     gene            4782..>5515
                     /gene="fliI"
                     /locus_tag="VI319_07040"
     CDS             4782..>5515
                     /gene="fliI"
                     /locus_tag="VI319_07040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835723.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HEY6863641.1"
                     /translation="MNLERYLAAVREADLCDRTGRVTHSYGLVVEAKGPDAFVGEVCD
                     ICPGTRAERVPAEVVGIKDGKVLLMPYSGLHGVRLGSEVIATGKPLQIPVGDALLGRV
                     VDAFGSPLDGRPAPACSGRYGFRAEPLNPLSRASIRDVLETGVKAIDALLTTGKGQRV
                     GIFSGSGVGKSTLLGMIARKVRADVNVIALVGERGREVREFIDRVLGAEGIARSVVVV
                     ATSDQPALVRSSAAYSATAMAEYFRD"
BASE COUNT          867 a         1909 c         2026 g          713 t
ORIGIN      
        1 gtcgacgtgg tggtcacgtc gatcggcgac gcgcgcagcc tgctcggcgg cacgctgctg
       61 ctcacgccgc tgaagggcgc agatgccgtc gtgcacgcgc tcgcgcaggg tccgctctcg
      121 gtgggcggct acaagtacga cctcaacggc aatgtcgtgc agaagaatca tccgaccgtg
      181 ggcgcgatcc cgagcggcgc gacggtcgag ctcgcggtcg ccaccgaggt ggtgaaggac
      241 gaccatctga gcttcgtcct gtccgatccc gactatacga ccgcgagccg cgtcgccgac
      301 tcgctcaaca ccacgctcgg gtcgggcgcg gtcgcgcggg cagtcgacgc tggccgcatc
      361 gagatcgcga ttccgccgga agagcgcgcc cgactggtca cgtacatgac ccgcatcgag
      421 aacgtcagca tcgagcccga ccagcgcgcg aaggtggtgg tgaacgagcg caccggcacg
      481 gtcgtgtccg gtggcgacgt gcgcatctcg cacgtcgtcg tctcgcacgg cgacctgaag
      541 gtttcgatca ctaccgacta cctggtgtcg cagccgacgg gagtggcgtt caccgggccg
      601 ggagtgcgca cggcggtcgt gccgcgcacc cgcatcgagg tgcaggaggc gcccgcggac
      661 ggcaacgtcg cggtggtcga ggtgagcaac acggtcgccg acctcgtgaa ggcgctcgcg
      721 cgcatcaaga cgcaaccgcg cgacatgatc tcgatcctgc agggcatcaa ggcggccggc
      781 gcgctgcatg ccgacctcat catccagtga cgcaaggggg aatctgacga tggacacggg
      841 agcactcacc ggcgcgacgg gcgcgttgat ccgcatggcg ctcgacggcg tcagcatgcg
      901 ccagctcgcc accgcgaaca acatcgccaa cgcgagcacg tccgattacg cgccggtgca
      961 cgtggacttc gagcagcagc tcgcggacgc gatgcgcgat gccgcgcgcg gccgcggtcc
     1021 cgatcccgcg cgcctgctgc ccacggtcac gaccgatccg acgcccggcg cgaaggtgca
     1081 gctcgacctc gaggcgacgc agatggcgca ggacgtgctg cattaccagg cgctgctgcg
     1141 cgcgctgcgc gagcagatgg atgtactgca atccgcgatc aacgacggga ggaaatgatg
     1201 gactatctgg ccgcattcgc aatcagcggt tccggcatga cggtcgagaa gatgcggctc
     1261 gacgtcacgg ccatgaacct cgcgaacgtc aacagctcga gaggcgccga cggcacgatg
     1321 ttccagccgc tgcgcgtcgt atcgggggtg cgcaccgacc ctcgcttcga ggccgcgatg
     1381 gcggcctggg gcgtgcagct cggcggcgcg caggtgcagc agctcgtgcc gacggacgca
     1441 ccgccgcgga tggtgctcga gcccgggcat cccgacgcgg actcgcgcgg cttcgtctcg
     1501 tatcccggca tcaacccggt cgccgagatg accaatctca tcaccgcggt gaggacctac
     1561 gaggcgaacg tcgtagcgct caacgccgcg aagacgatgg cgcagagagc gctcgacatc
     1621 ggcggaggcg gccgatgagc atcgacgcgg tcggcttcat cccttcggcg acggcgcagg
     1681 cgatcgagtc gaagctcccc gggtcggcag tcgggaccgg aaccgggacc ggggccgcgc
     1741 ccgcgtccgg cttcggcgac tggctcgcga gcgagatcgc acaggtgaac gacaagatcc
     1801 agagcgcgga caagaaggtg caggacctcg cgctcggcaa ggcgagcaac ctgcacgacg
     1861 tgatgatcgc gctcgaggac accaagctct cgttccagct catggtgcag atacgctcgc
     1921 gcgtgctgga agcgtaccag gacatcctgc gcatgcaggt ctgaggagcg gacatggcga
     1981 acgcaagaga acgctgggct gctctcggcg cgggcgcgaa ggtgagcatc gtgacggggt
     2041 tgctcgtcgt cgtggtgctg ctgatcgcgg gcgccgcgtg gatcctgcgc gacgactacc
     2101 aggtgctgtt cgcggatctc gacccgcagg acggcgcagc catcgtcgcc gagctcgacc
     2161 gcatgaaggt cggctaccgc ctcgcggaca gcggccgcac catcctggtc gagcgcgacg
     2221 ccgtgcacaa gacgcggctg aagatcatgg gcggcaaagg cgtgccgctc aagggcaacg
     2281 tcggcttcga gatcttcaac aacagcgact tcggcatgac cgagttcgcg cagaagatca
     2341 actaccagcg cgcgctgcag ggcgagctcg cgcgcacgat catgagcatc gaggagatca
     2401 ggcaggcgcg cgtgcacctc gccttcccgg agggcgggct gctgaagaag aacagcgcgc
     2461 agcccaaggc ctcggtcacg ctcgcgcttc gcggcgacag ccgcctgcgg cccgagcaga
     2521 tcgtcggcat ccagcgcctg gtcgcggccg ccgtgcccga catggacccg caggcggtga
     2581 cggtgctcga tcaccagggt gtcgcgctca gccggctcgc cgacgagcgc gacgacatgg
     2641 gaggggtcgg cggccggctc gagctgaaga agagcacgga ggtctatctc acgaagaagg
     2701 cgatcgaggt gctcgatcgc gcgttcggtc ccggccaggc catcgtcagc atcgacgtca
     2761 cgctcagcca ggacagcgtg aagaccacgc gcgaggacgt gatccccttc gcgcgcaagg
     2821 acggggagtt cgccggagcc gtgacccggc gccgcaccaa cgtgcagggg gtggcgaccc
     2881 cgcgcggcta cgaggtccag gccaccgccg acctcgccaa gcagaccccg cagccggtcg
     2941 gaacgcagac ctccagcgtc gaggtcgagt acgagaacgg caagcaggtg gagcaggtcg
     3001 tcaccacgcc gggaagcatc aagcgcatca gcgtcggcgt cgtccttccc gggcggatgg
     3061 acgcgcagcg catccagcgc gtgtcggacg tcgtcgcgat ggcagtcggc ctgcagcgcc
     3121 agcgcggcga cgccagcgcg gtgcacgcgg tcgacgagct gggcgcgcgc tccgtttccg
     3181 gtttgccggc ggcgctgccc gagccggcgc cgtcggagga gccgccgcag gcgcgcgcgg
     3241 ttgcgcgcga cgccggcccg aaatcggtcg acctttcggt gccgatcgcc gccgcgctcg
     3301 cgatgctgct cgccgtggcg ctcgcgctgt tcgtcgcggc gtcgcggcgc cggccggagc
     3361 cgcccacgat gaccgcggag gaccgcgaga aggcgctttc gcagatcagg gcctgggtgc
     3421 agggcggcga gccgacgccg acgcaggaga cgaggtcatg agcgacggat tgcggctcgc
     3481 ggccctcagg ctgcacggcg tcgccgcggc cgaccgcgac tggatcctcg accgcctggg
     3541 ccggcgcgac cggcgggcgc tggggctgct gctgcgcgac ctgaagcgct cgggcatcgt
     3601 cgcggcgcac gtcgaggcgg cgaaggagga gctcgcggcc gctccggagc gcgagcgcct
     3661 cgagccggta gccccgggcg tgccggccga tctcgcgcac gccgaccccg aggccgtggc
     3721 gcgcgctctg agcggcgagc ccgaatgggt acgctcgacg atcctcgcga tgcatcactg
     3781 gaaatggcgc cgggcggtgc tgcagcgcct cgcggcgggc gcccccgatt cgagcgtccg
     3841 atccgcgccg gtcgctgcgg cgccgcaccc ggcgttcgct gccgcgctcg cgcgctcgct
     3901 cgcgcgcagg atcggcgaga cgtcggccga gctcgagacc cgcggcgcgt ccttcgagcg
     3961 agtcatggac aacctcgatc cgctcgcgcg cagcccgcgg ccgcgcatct gggaaaggct
     4021 gcgcgcatgg atgcgctgat ccgcggcgcc cacgtttccg agcgcgccgt cgtcctgggc
     4081 gtgcgccgtc cggggcatgc cgaagcggag cccgtggcgc gcccagcgcc cgcggggacc
     4141 ggcgccgacc cgtgcccggt cggtgcgcgc gtcgaatcgg cggctcccga ggccgcatgg
     4201 ctcgaaagcg agcgcagaaa gatcgcggac gagctgcggc tcgagctcga cgcgcgcctg
     4261 gaacgcgagg cggaggaggc gcgcaaggcc ggtcacgccg aaggcgtcga gcagggccgg
     4321 gccgaggcgc tgcgcgaggc gagcgagcag gcggccgcgg ggacgcgcgc gctcgccgcg
     4381 gtcgcgaagc acgccgagcg cgagatcgaa ggggcgcagg accttctcgt cggcatcgcc
     4441 ttcgaggccg tctgcaagct gctcggcgcc agggcgctcg agcgcgaggc ggtcgccggg
     4501 atggttcacg aggtgatcgc ccgggtgaag cgcgacgagg cgatgctcgt gcgcctgcac
     4561 ccgcaggact gcgcgctgct gcgcggcgcc gccggcgacc cgcgcggcga tggcgaccgg
     4621 cacttcaagg tggagctggt cgcggacgac aaggtcgcgc tcggcggctg cctcgtcgag
     4681 accgagggcg gcttgctcga tgcccggctc gagacgcagc tcgagcgcct gcgcgccacg
     4741 ctgctcgacg cacgcgcgtc cacgcgcccg gcggagcatc cgtgaacctc gagcgctacc
     4801 tggccgccgt gcgcgaggcc gatctgtgcg accggaccgg acgcgtcacg cattcctacg
     4861 gactggtcgt cgaagccaag ggacccgacg cgttcgtcgg cgaagtgtgc gacatctgtc
     4921 ccggcacgcg cgccgagcgc gtccccgccg aagtggtcgg catcaaggac ggcaaggtgc
     4981 tgctcatgcc ctacagcggg ctgcacggcg tgcgcctcgg aagcgaggtg atcgcgaccg
     5041 gcaagccgct gcagatcccg gtcggcgatg cgctcctcgg ccgcgtcgtc gacgccttcg
     5101 gctccccgct cgacggacgg ccggcgcccg cgtgcagcgg gcgctacggc ttccgcgccg
     5161 agccgctcaa tccgctctcg cgagcgtcga tccgcgacgt gctcgagacc ggcgtgaagg
     5221 cgatcgacgc gctgctcacg accggaaagg gacagcgggt cggcatcttc tccggcagcg
     5281 gcgtcggcaa gagcaccctg ctcgggatga tcgcgcgcaa ggtccgcgcc gacgtcaacg
     5341 tcatcgcgct cgtcggggag cgcggtcgcg aggtccgcga gttcatcgat cgcgtgctcg
     5401 gcgccgaggg catcgcgcgc tcggtggtgg tcgtggcaac gtccgaccag ccggcgctgg
     5461 tgcgatcgag cgccgcttac tcggcgaccg cgatggccga gtacttccgc gacca
//
LOCUS       DATDMW010000162         4774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1791803, whole genome shotgun sequence.
ACCESSION   DATDMW010000162 DATDMW010000000
VERSION     DATDMW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791803"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VI319_07045"
     CDS             <1..516
                     /locus_tag="VI319_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863642.1"
                     /translation="QVRRVVTKLDDSGKAVVMIDERTPLTAPRPPNYAANIWVTSTNL
                     PDFTNTADRGKTKIGLVPPNGGTVFRIVDFAPDSQGEHPTDMNHMMKIVGAGAPKRGL
                     PPRHPMMHRTRSLDYGIVMSGEIDMLMDEGEVHFKAGDVFVQQATNHAWVNRGKVPAR
                     VAFILIDSQEP"
     gene            complement(532..1473)
                     /locus_tag="VI319_07050"
     CDS             complement(532..1473)
                     /locus_tag="VI319_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6863643.1"
                     /translation="MEKRAFLKMTTLAVAGMTFGPARAVSAATRFDPRAKLDVKITEV
                     PYRKNAAGRQLLARIYQPQGAGPFPVLLDLHGGAWNRKDRKAEEPMDRAIAESGVLVV
                     AIDMTIAKEAPYPACVQDAHYGVRWLKSKAAGWKGDPATLGVFGSSSGGHVAELIGMR
                     PRDARYAAYPLKEAPNLDASVLYVATRSPISNTFARYQNAVNRKRENMMRNNTNFFKP
                     FDTIHEANPQEILERGEKVVMPPLLIMQGALDDNVLPPDQKKFAETYKAKGGDVRFEL
                     FEGCEHEWVAEPGPQTDRAHEMVKGFIAQQLSARRSA"
     gene            complement(1489..1740)
                     /locus_tag="VI319_07055"
     CDS             complement(1489..1740)
                     /locus_tag="VI319_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863644.1"
                     /translation="MKNSEVPMMTLRRAVQLCDGIDALGDALSVSTATLERMISGDEE
                     VPLDVFTQAVGLLLEVTATRTPKRSTPDSNDGGRKGPRH"
     gene            complement(1764..2783)
                     /gene="waaF"
                     /locus_tag="VI319_07060"
     CDS             complement(1764..2783)
                     /gene="waaF"
                     /locus_tag="VI319_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEY6863645.1"
                     /translation="MPRILIVAPNWIGDTLLAQPLFARLRRKLPSAVIDAMAPPWTAP
                     VLRRMPEISDVIEAPFLHGRLELRARWRLGRSLRAHQYDEAIVLPNTFKAALVPFFAE
                     IPLRVGYVGETRYGLLTLAHKLDEKKLPLMAERYAQLAERPGAAPLRPLAPAKLNVDE
                     ANLIITLGRLGLSRAKPVAALCPGAEYGPSKRWPAGHFAALAKKLAAMGRAVWIFGSP
                     NDRPIGEEIAKLADGAAVNLCGRTDLAAAIDLLSIAEIVITNDSGLMHLAAAVGRPVV
                     ALYGSSSPDHTPPLTKRARIVRTGIDCSPCFARDCPLGHHKCMVDLTPERVLREIADI
                     EAKVH"
     gene            complement(2820..3017)
                     /locus_tag="VI319_07065"
     CDS             complement(2820..3017)
                     /locus_tag="VI319_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY6863646.1"
                     /translation="MGADDRSPRVVSVTEADLPLHCPTPSTPVWSMHPRVFLDVADTG
                     KMLCPYCGTLYVYKGAPAKGH"
     gene            complement(3022..3975)
                     /locus_tag="VI319_07070"
     CDS             complement(3022..3975)
                     /locus_tag="VI319_07070"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY6863647.1"
                     /translation="MSMADRDGVIWYDGKLVPWRSATTHVLTHSLHYGLSVFEGVRAY
                     KTVDGTAIFRLKEHTERLFNSAHIYMMKIPFAREQLIEAQKEVVRENKHESCYVRPIA
                     FYGSEKMGVSPIGASVHVAIAAWPWGAYLGAEAIEKGIRVKTSSYARHHINVTMARAK
                     MAGTYPNSILANLEATQHGYDEGLLLDVDGFVAEGSGENLFIVKDGKLHEPELTSALS
                     GITRASVIELANELGYEVISRRITRDDIYIADEAFFTGTAAEVTPIRELDGRIIGAGK
                     RGPVTTQIQKLFFDVVAGKVAKHKDWLTPVDAKQPAGRVKA"
     gene            complement(4033..>4774)
                     /locus_tag="VI319_07075"
     CDS             complement(4033..>4774)
                     /locus_tag="VI319_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEY6863648.1"
                     /translation="GGKELGYASDLDLIFLHDDEDERAQEIYARLAQRMSRLLTSRTA
                     AGVLFDIDLRLRPDGEGGMVVSSIDAFRRYQREAAWTWERQALTRARFSAGDATVGAA
                     FEDERRRVLREPRDAAKLREDVLSMRTQLLEGHPNTSELFDLKHDRGGMIDIEFVVQY
                     LVLAHAHAHPELVANDGNIALLRIAARLGLIATETAETVGDAYREFRRLQHKLRLNGA
                     RYARVPAEEVRASTGATLALWREVFGAD"
BASE COUNT          687 a         1664 c         1659 g          764 t
ORIGIN      
        1 caggtgcggc gcgtggtcac caagctggac gactcgggca aagccgtggt gatgatcgac
       61 gagcgcacgc cgctcaccgc gccgcgcccg cccaactacg cggccaacat ctgggtgacg
      121 agcacgaacc tccccgactt caccaacacc gcggaccgcg gcaagaccaa gatcgggctg
      181 gtgccgccga acggcggcac ggtgttccgc atcgtcgact tcgcgcccga ctcgcagggc
      241 gagcacccga ccgacatgaa ccacatgatg aagatcgtcg gcgccggggc gcccaagcgc
      301 ggcctgccgc cgcgccaccc gatgatgcac cgcacgcgca gcctcgacta cggcatcgtc
      361 atgtcgggcg agatcgacat gctgatggac gagggcgagg tgcacttcaa ggcgggcgac
      421 gtgttcgtgc agcaggcgac gaaccacgcc tgggtgaacc gcggcaaggt gcccgcgcgc
      481 gtggccttca tcctgatcga ctcgcaggag ccctgagcag ggctcctgtg cctacgccga
      541 gcgccgcgcc gagagctgct gcgcgatgaa ccccttcacc atctcgtgcg cgcggtcggt
      601 ctgcgggccg ggctcggcga cccactcgtg ctcgcagccc tcgaagagct cgaagcgcac
      661 gtcgccgcct ttggccttgt aggtctcggc gaacttcttc tggtcgggcg gcagcacgtt
      721 gtcgtcgagc gcgccctgca tgatgagcag cggcggcatc acgactttct ccccgcgctc
      781 gaggatctcc tgcgggttcg cctcgtggat ggtgtcgaac ggcttgaaga agttggtgtt
      841 gttgcgcatc atgttctcgc gcttgcggtt caccgcgttc tggtagcgcg cgaaggtgtt
      901 gctgatcggc gagcgcgtcg cgacgtagag gacgctcgcg tccagattcg gcgcctcctt
      961 cagtggatac gcggcgtagc gcgcgtcgcg cgggcgcatg ccgatcagct cggcgacgtg
     1021 gccgccgctc gagctgccga acacgccgag cgtcgccggg tcgcccttcc atccggccgc
     1081 cttcgacttc agccagcgca cgccgtagtg cgcgtcctgc acgcacgcgg ggtagggcgc
     1141 ttccttggcg atggtcatgt cgatcgcgac gacgagcacg ccgctctcgg cgatcgcgcg
     1201 gtccatcggc tcctcggcct tgcggtcctt gcggttccac gcgccgccgt gcaggtcgag
     1261 cagcacgggg aacggccccg cgccttgcgg ctggtagatg cgcgccagca gctgccgccc
     1321 ggccgcgttc ttccgatagg gcacctcggt gatcttcacg tcgagcttcg ccctcggatc
     1381 gaatcgggtc gccgcggaca cggcgcgcgc cggcccgaag gtcatgccgg cgaccgccag
     1441 cgtggtcatc ttgagaaatg cgcgcttttc catgccggtc tcctttcgct agtgccgcgg
     1501 gcctttcctg ccgccgtcat tcgaatcggg cgtcgagcgc ttcggcgtgc gcgtggcggt
     1561 gacctcgagg agcaggccca cggcctgcgt gaacacgtcg agcggcacct cttcgtcgcc
     1621 ggagatcatg cgctcgagag tggcggtcga cacgctgagc gcgtcgccga gagcatcgat
     1681 gccgtcgcag agctgcaccg cgcggcgcaa ggtcatcatc ggaacttcgg agttcttcat
     1741 gccgcatcat atcccgaacg gcctcagtgg accttcgcct cgatatccgc gatctcgcgc
     1801 agcacgcgct cgggcgtcag gtcgaccatg cacttgtgat gcccgagcgg gcagtcgcgc
     1861 gcgaagcacg ggctgcagtc gatgcccgtg cgcacgatgc gcgcgcgctt ggtgagcggc
     1921 ggcgtgtgat ccgggctcga cgacccgtag agcgcgacga ccgggcggcc caccgcggcc
     1981 gcgaggtgca tgagccccga gtcgttggtg atgacgatct cggcgatcga gagcaggtcg
     2041 atcgcggccg cgaggtcggt gcggccgcac aggttcaccg ccgcgccgtc ggcaagcttc
     2101 gcgatctctt cgccgatcgg gcggtcgttc ggcgagccga agatccagac cgcgcggccc
     2161 atcgccgcga gcttcttcgc gagcgccgcg aagtggcccg ccggccagcg cttcgaaggc
     2221 ccgtactcgg cgccggggca gagcgccgcg accggcttcg cgcgcgacag ccccaggcgc
     2281 ccgagcgtga tgatcaggtt cgcctcgtcg acgttcagct tcgccggcgc cagcggccgc
     2341 agcggcgccg cgcccggacg ctcggcgagc tgcgcgtagc gctcggccat gagcggcagc
     2401 ttcttctcat cgagcttgtg cgcgagcgtc agcagcccgt agcgcgtctc gccgacatag
     2461 ccgacgcgca gcgggatctc ggcgaagaac gggacgagcg ccgccttgaa ggtgttcggc
     2521 agcacgatcg cctcgtcgta ctggtgcgcg cgcagcgagc ggccgagccg ccagcgcgcg
     2581 cgcagctcga gcctgccgtg caggaacggc gcctcgatca cgtcgctgat ctcgggcatg
     2641 cgccgcagca cgggcgcggt ccacggcggc gccatcgcgt cgatcaccgc gctcggcaat
     2701 ttccggcgca gccgcgcgaa cagcggctgg gccagcagcg tgtcgccgat ccagttcggg
     2761 gcgacgatca ggattctggg cacttcggtt ccgcgtcggg cggccgtgcc gccgggagtt
     2821 caatgcccct tcgccggggc gcccttgtag acgtacagcg tgccgcagta cgggcacagc
     2881 atcttgccgg tgtcggcaac gtcgaggaag acgcgcgggt gcatgctcca caccggcgtg
     2941 ctcggggtcg ggcagtgcag cggcaggtcc gcctcggtga ccgagacgac gcgcgggctg
     3001 cgatcgtcgg cgcccatcgc ctcaggcctt cacgcggccg gcaggctgct tcgcgtcgac
     3061 cggcgtcagc caatccttgt gcttcgcgac cttgccggcg accacgtcga agaacagctt
     3121 ctggatctgc gtcgtcaccg ggccgcgctt gccggcgccg atgatgcggc cgtccagctc
     3181 gcggatcggc gtcacctcgg cggcggtgcc ggtaaagaaa gcctcgtcgg cgatgtagat
     3241 gtcgtcgcgc gtgatccggc gcgagatcac ctcgtagccg agctcgttcg cgagctcgat
     3301 caccgacgcg cgcgtgatgc ccgagagcgc cgaggtcagc tcgggctcgt gcagcttgcc
     3361 gtccttgacg atgaacaggt tctcgcccga gccctcggcg acgaagccgt cgacgtcgag
     3421 caggaggccc tcgtcgtagc cgtgctgcgt cgcctcgagg ttcgcgagga tcgagttcgg
     3481 atacgtgccc gccatcttcg cgcgcgccat cgtcacgttg atgtggtgcc gcgcgtacga
     3541 cgacgtcttc acgcggatgc ccttctcgat cgcctcggcg ccgagatagg cgccccaggg
     3601 ccaggccgcg atcgcgacgt gcacgctcgc gccgatcggc gatacgccca tcttctccga
     3661 gccgtagaac gcgatcggcc ggacgtagca ggactcgtgc ttgttctcgc gcaccacttc
     3721 cttctgcgcc tcgatcagct gctcgcgcgc gaagggaatc ttcatcatgt agatgtgcgc
     3781 cgagttgaac agccgctcgg tgtgctcctt gagccggaag atcgccgtgc cgtcgaccgt
     3841 cttgtacgcg cggacgccct cgaacacgga caagccgtag tgcagggaat gggtgagcac
     3901 gtgcgtggtc gcggagcgcc acggcacgag cttgccgtcg taccagatca ccccgtcgcg
     3961 gtcggccatc gacatcggaa actccggaaa cgcctgggaa ggggacattc tagcggaaag
     4021 cccgcccgct ccctagtcgg cgccgaacac ctcccgccag agcgcgagcg tcgcgccggt
     4081 gctcgcgcgc acctcctcgg ccggcacgcg ggcgtagcgc gcgccgttca gccgcagctt
     4141 gtgctggagg cggcggaatt cgcggtaggc gtcgccgacc gtctcggccg tctcggtggc
     4201 gatcaggccg agccgcgccg caatccgcag cagggcgatg ttgccgtcgt tcgcgaccag
     4261 ctcgggatgc gcatgcgcgt gcgcgagcac caggtactgc acgacgaact cgatgtcgat
     4321 catgccgccg cggtcgtgct tgaggtcgaa cagctcgctc gtgttgggat gcccctcgag
     4381 gagctgcgtc cgcatcgaga ggacgtcctc gcgcagcttc gcggcgtcgc gcggctcccg
     4441 cagcacgcgc cggcgctcgt cctcgaacgc ggcgccgacg gtggcgtcgc cggccgagaa
     4501 gcgcgcgcgc gtgagcgcct ggcgctccca ggtccacgcc gcctcgcgct ggtagcggcg
     4561 gaaggcgtcg atcgagctca cgaccatgcc gccctcgccg tcggggcgca ggcgaaggtc
     4621 gatgtcgaac agcacgccgg cggcggtgcg gctggtgagc aggcggctca tgcgctgcgc
     4681 gagcctcgcg tagatctcct gcgcgcgctc gtcctcgtcg tcgtgcaaga agatgaggtc
     4741 gaggtcggaa gcgtagccga gctccttgcc gccg
//
LOCUS       DATDMW010000163         3581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_296130, whole genome shotgun sequence.
ACCESSION   DATDMW010000163 DATDMW010000000
VERSION     DATDMW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296130"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="VI319_07080"
     CDS             <1..115
                     /locus_tag="VI319_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6863649.1"
                     /translation="GRLGTSEEVASVFAFLASDEASYVNGAVISVDGGMTL"
     gene            complement(143..2854)
                     /locus_tag="VI319_07085"
     CDS             complement(143..2854)
                     /locus_tag="VI319_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEY6863650.1"
                     /translation="MALRDLVSRHYLTPLFEPQSVAIVGATDKKGKVGEVLIANMLEA
                     GFKGPLYAINPKYKEVCGVPSFASIDKLPQRVDLAVIATPPQTVPGVIDECGRAGVRA
                     AVVITAGFSETGPAGAKLERALLENARRHGVRVIGPNCLGILRPALGLNATFARSRVV
                     PGSLGLISQSGAVCTAMLDWAEPGGVGFSSVVSLGGSVELDFGEIIDYLANDPATEHI
                     LLYIEGIRDARRFLSSLRAAARMKPVILMKVGRHPAGSRAAVSHTGAIVGADDVFDAV
                     VKRAGVVRVRSVGQLVAAAQALSSHVRPRGNRLAVVTNGGGPGVMAADLAADLELPLA
                     ELSSATIDSLRDALPPNWSHGNPLDLIGDADSARYRAAVKACLGDDGVDGLLTILTPQ
                     AMTDAEDVARAVVESARGSSKPLVACWMGEAHVASARKILTDAGIPTFRTPDPAVEMF
                     AHVSAFYRNQQALLQAPGPLARGSAPDLRGARLVVEAALADRRKVLTEMESKALLAAF
                     HVPIARTAIARSAQEAVLLAEQFGFPVAMKIDSPDVTHKSDVGGVRLNVADAQSARAA
                     YQEIVEQVKRKRPGARVNGIAIEPMMVRPHGRELMVGVKRDAVFGPAITFGAGGTAVE
                     VHRDRSVALPPLNAFLVADMIRGTRVSRLLGAFRNLPPADTKALESVLLRVSEMVCEL
                     PQIEELDINPLIVDDKGAVVVDARVVVREAPPMRRYAHMAIHPYPSELCTTWQPREGP
                     PVTLRPIRPEDAQMEQEFVKGLSDQSRHFRFMNTLKELTPAMLARFTQIDYDREVAII
                     AVQELDRKEIELGVARYVMNPDGETCEFAIVVADEWQGKGLARRMLEMLVDVARSRGL
                     KAIIGHILSMNQPMIKLSEKLGFTIADGVDDPTTKRATLALERAIGR"
     gene            complement(2857..3123)
                     /locus_tag="VI319_07090"
     CDS             complement(2857..3123)
                     /locus_tag="VI319_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863651.1"
                     /translation="MAKDDKRIDLEQVRTMLEALERDLAAVRGGSQDLQVLRDEVDRL
                     KALLESGEPHHTVRDSLHSLRLALQGEGIRLGQYVGMIGRILGM"
     gene            3269..3523
                     /locus_tag="VI319_07095"
     CDS             3269..3523
                     /locus_tag="VI319_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863652.1"
                     /translation="MNGRHRAQGSSGNAVPRQLPLAFEALASPGTESALRAAYRRLAI
                     SRRLSYEQAMSQRAYEIGIRNLAEAIARRIRHRRSRRGRP"
BASE COUNT          500 a         1288 c         1275 g          518 t
ORIGIN      
        1 cgggcgcctg gggacctccg aggaggtcgc cagcgtgttc gccttcctcg cctcggacga
       61 ggcgagctac gtcaacggcg cggtgatcag cgtcgacggc ggcatgacgc tgtagtcggc
      121 gtcgcaggcg ccgcgacgcg cgtcagcgcc cgatcgcgcg ctcgagcgcg agcgtcgccc
      181 gcttcgtggt cgggtcgtcg acgccgtcgg cgatcgtgaa cccgagcttc tccgagagct
      241 tgatcatcgg ctggttcatc gacaggatat ggccgatgat cgccttcagc ccgcgcgagc
      301 gcgcgacgtc gaccagcatc tcgagcatgc gacgcgcgag gcccttgccc tgccattcgt
      361 cggccaccac gatggcgaac tcgcaggtct cgccgtccgg attcatgacg tagcgcgcga
      421 ccccgagctc gatctccttc cggtcgagct cctggaccgc gatgatcgcg acctcgcggt
      481 cgtagtcgat ctgggtgaag cgcgcgagca tcgcgggcgt cagctccttc agcgtgttca
      541 tgaagcggaa gtgccggctc tggtccgaga gccccttcac gaactcctgc tccatctgcg
      601 cgtcctcggg ccggatcggg cgcagcgtca ccggcggccc ctcgcgcggc tgccacgtgg
      661 tgcacagctc gctcgggtag gggtggatcg ccatgtgcgc gtagcggcgc atcggcggcg
      721 cctcgcgcac gaccacgcgc gcgtcgacga ccacggcgcc cttgtcgtcg acgatgagcg
      781 ggttgatgtc gagctcctcg atctgcggca gctcgcacac catctcggac acgcgcagca
      841 gcacgctctc cagcgccttg gtatcggcgg gcggcaggtt gcggaaggcg ccgagcagcc
      901 tggagacgcg cgtgccgcgg atcatgtcgg cgacgaggaa ggcgttgagc ggcggcagcg
      961 cgaccgagcg gtcgcggtgc acctccaccg ccgtgccgcc cgcgccgaag gtgatcgccg
     1021 ggccgaacac ggcgtcgcgc ttcacgccga ccatcagctc gcggccgtgc gggcgcacca
     1081 tcatcggctc gatggcgatg ccgttcacgc gtgcgcccgg gcgcttgcgc ttcacctgct
     1141 cgacgatctc ctgataggcc gcgcgcgcgc tctgggcgtc ggcgacgttg agccgcacgc
     1201 cgccgacgtc gctcttgtgc gtgacgtcgg gcgaatcgat cttcatcgcc accggaaagc
     1261 cgaactgctc cgcgaggagc accgcctcct gcgccgagcg cgcgatcgcg gttctcgcga
     1321 tcgggacgtg gaacgcggcg agcagcgcct tcgactccat ctcggtgagg accttgcgcc
     1381 ggtcggcgag cgccgcctcg accaccaggc gtgcgccgcg caggtcgggc gccgagccgc
     1441 gcgcgagcgg ccccggcgcc tgaagcagcg cctgctggtt gcggtagaag gccgagacgt
     1501 gcgcgaacat ctcgaccgcc gggtcgggcg tgcggaaggt cgggatgccg gcgtcggtga
     1561 ggatcttgcg cgcggaggcg acgtgcgcct cgcccatcca gcacgcgacg agcggcttcg
     1621 aggacccgcg cgcgctctcg accacggcgc gcgccacgtc ctcggcgtcg gtcatcgcct
     1681 gcggcgtcag gatcgtgagc agcccgtcga cgccgtcgtc gccgaggcag gccttcaccg
     1741 ccgcgcggta gcgcgcgctg tcggcgtcgc cgatcaggtc gagcgggttg ccgtgcgacc
     1801 agttcggcgg cagcgcgtcg cgcagcgaat cgatggtcgc gctcgagagc tcggcgagcg
     1861 gcagctcgag gtcggcggcg aggtccgccg ccatgacgcc cgggccgccg ccgttggtga
     1921 cgaccgcgag acggttgccg cgcgggcgca cgtgcgagga gagcgcctgc gccgcggcga
     1981 cgagctgccc cacggagcgc acgcgcacga cgccggccct tttcacgacc gcatcgaaga
     2041 cgtcgtcggc gccgacgatc gcgccggtgt gcgagaccgc ggcgcgcgag cctgccgggt
     2101 ggcggccgac cttcatcagg atcacgggct tcatgcgcgc cgccgcgcgc aggctcgaca
     2161 ggaagcgccg cgcgtcgcgg atgccctcga tgtagagcag gatgtgctcg gtcgcgggat
     2221 cgttcgcgag gtagtcgatg atctcgccga agtcgagctc gaccgagccg ccgagggaaa
     2281 cgacgctgga gaagcccacg ccgcccggct cggcccagtc gagcatggcg gtgcacaccg
     2341 cgcccgactg cgagatcagc ccgagcgacc cggggacgac gcggctgcgc gcgaaggtcg
     2401 cgttgagccc gagcgcgggc cgcaggatgc cgaggcagtt gggcccgatc acgcgcacgc
     2461 cgtggcggcg cgcgttctcg agcagcgcgc gctcgagctt cgcgcccgcg ggacccgtct
     2521 ccgagaaccc ggcggtgatc acgaccgcgg cgcgcacgcc ggcgcggccg cactcgtcga
     2581 tcacgccggg cacggtctgc ggcggcgtcg cgatcacggc caggtccacg cgttgcggca
     2641 gcttgtcgat cgacgcgaag gacgggaccc cgcagacctc cttgtacttc gggttgatcg
     2701 cgtacagcgg ccccttgaag ccggcctcga gcatgttcgc gatcaggacc tcgccgacct
     2761 tgcccttctt gtcggtcgcg ccgacgatgg ccaccgactg cggctcgaac agcggcgtca
     2821 ggtagtgacg cgagacgagg tcgcgcagcg ccacggtcac atcccgagga tgcgcccgat
     2881 catcccgacg tactgcccga gccggatgcc ctcgccctgg agcgcgagcc gcagcgaatg
     2941 cagcgagtcg cgcaccgtgt gatgcggctc gccggactcg agcagcgcct tgagccggtc
     3001 gacctcgtcg cgtagcacct gcaggtcctg cgacccgccg cggaccgccg ccaggtcgcg
     3061 ctcgagcgcc tcgagcatcg ttcggacctg ctccaggtcg atgcgtttgt cgtctttcgc
     3121 catgacgtca ttctccatct tcggccggcc gtgcgcagcc cggcatcgcc tcattttgat
     3181 gtgcatcaga acgtccgcgc cgcggcgctg ataccctgag agcgtcctca atcacccgaa
     3241 gtttctcgca aacctcgcgg agacgggcat gaacgggcgg caccgcgcgc agggctcctc
     3301 cggcaacgcc gttccccgtc aattgcccct cgcgttcgaa gcgctcgcgt ccccggggac
     3361 cgagtcggcg ctgcgcgccg cgtaccggcg cctcgcgatc tcgcggcggc tcagctacga
     3421 gcaggcgatg tcgcaacggg cgtacgagat cgggatccgc aatctcgccg aggcgatcgc
     3481 gcgccgcatc cggcaccggc gttcgcgccg cggtcgaccg tagacgggcg ccggaatctt
     3541 cgagcgcgcg agggcgaggc cggatcgtac gcgcggcagc c
//
LOCUS       DATDMW010000164         7295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1417407, whole genome shotgun sequence.
ACCESSION   DATDMW010000164 DATDMW010000000
VERSION     DATDMW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417407"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..721)
                     /locus_tag="VI319_07100"
     CDS             complement(104..721)
                     /locus_tag="VI319_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY6863653.1"
                     /translation="MKRAALVLVALFIVEALAACGGGSSPKLAKLPGDAVVLAFGDSL
                     TYGYGANPNESYPARLQALIGRKVVSAGIPGETTAGGLARLQSALDESKPALLILCEG
                     GNDFLQHLDEKIAAQNLRSMIGLARAQGAQVVLIGVPKPGLMPSTATLYTDIATEMKI
                     PYDNSTLVKILTDNELKSDLVHPNAKGYAKMAEGVAALLKKSGAI"
     gene            complement(718..2502)
                     /locus_tag="VI319_07105"
     CDS             complement(718..2502)
                     /locus_tag="VI319_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY6863654.1"
                     /translation="MDSRVARPVFLRTLPFLKWWPLVDRRTLRADVIAGFVGATVVLP
                     QGVAFATLAGLPPEYGLYASMVPVVIAALFGSSLHGMSGPTNPVSLMVLASLAGLAVP
                     GTPYYIHLALTLALMAGAIMIALGLLRLGSLLQFVSDTVVVGFTAAVALLVFASQLPD
                     FLGIRIPAASSFTALVLSTFIRLSHIQPWVVVVACATLAVGALAREFFPRVPPMLVAM
                     LAGGALAWALNAWLGPARTGLRTLGALPGALSPLSVPDVSPLTLRTLLGPALAVAAVC
                     LIQAYSVTRALALKSGQRVDYNQEFVGQGLANVSASFFSGMAVSMSVNRCVLNYESGA
                     RTPMAAILSALFLVLVLAAVGGLAAYLPYAVVAGMLFIVAWSLIDFARIRQIVRTSRG
                     ETTVLVITFAATLLFDMELAIALGVLASLVVYLHRTSHPIMRTLVPDPRHPNRKMTEP
                     EDDLRECPQAKILRIEGSIWFGAVSHVERHFDTLREHAKSQEHLILMAKSVNFVDMAG
                     AELLAEEAERRRAIGGRLYFYSLRQPVEDLLRRGGYMKRIGEENVFRGKQDAFATIFE
                     RLDRDVCATCRARIFVECKTLPPPKDPS"
     gene            2597..3772
                     /locus_tag="VI319_07110"
     CDS             2597..3772
                     /locus_tag="VI319_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6863655.1"
                     /translation="MLVCSSLILTLALGIRHSFGLFLTPMSLEHGWGREVFGLAIAIQ
                     NLLWGLSQPLTGMLADRYGAPRVLMAGGVLYAAGLVAMAYAPTPLVFTLSAGLLIGFG
                     MSSTTFNVVFGALGRAFPPEQRSKILGLGSSAGSFGQFVMLPLALFLISALGWHWAML
                     ALALTAATMIPAALGVPDQGYGGASSAGGLPLKAALGEATRHRGFWLLGVGYFTCGFQ
                     IVFIGTHFPSFLLDHGLSARDGTVALALIGLFNVFGSYLAGYLGGRFPKNYLLSGLYA
                     VRGLAIALLLAFPLTPFSVYCFAAGFGFTWLGTVPLTNGVVAGIFGVRHFAMLSGIVF
                     MFHQLGSFFGSWLGGWIYDHTGSYNAVWMIAIGLSVVSTLVNLPVEEKPVVRGPAAA"
     gene            3779..4585
                     /locus_tag="VI319_07115"
     CDS             3779..4585
                     /locus_tag="VI319_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY6863656.1"
                     /translation="MPRFCANISMLFAEREFPERFDAAARSGFAAVEIQFPYRWSREA
                     LAERARAAGVEIVQINLPAGDAQRGDRGIACDPSRAEEFRDGVGRALEYARALGCRQM
                     NCLAGIAPPGLPEAALRETFVGHLRWAAAALAREGMTLLVEPINTRTIPGFWLNRPSQ
                     ALALIDEAGAANLKLQFDVFHVHVMEGDVGPALEAAFAHIGHIQVADDPGRHEPGTGA
                     IDFPALFERIDGLGYRGWIGAEYVPSAATEDTLGWARRWVHEVPERRNPW"
     gene            4596..6086
                     /locus_tag="VI319_07120"
     CDS             4596..6086
                     /locus_tag="VI319_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6863657.1"
                     /translation="MNVDDLHQPIDGARQAEVVARLRAILPPAALLHAAEDTKSYESD
                     GLSAYRQPPMVVALPDNEDQVAAVLRACRDLGVPVVARGSGTGLSGGALPRGDGVLLS
                     LAKMRRVLAVDPVARVARVQPGVTNLAVSAAAEPYGLFYAPDPSSQIACSIGGNVAEN
                     SGGVHCVKYGLTVHNVLRARAVTIEGEVIELGSEAPDAPGVDLLALLIGSEGMLAVVT
                     EVTVKLLPRPETARCVLASFDDLEKAGNAVADIIAAGIIPAGLEMMDRPATRAVEDFV
                     HAGYDLEAKALLLCESDGTPEEVEEEVARVTAVLERAGATRFRVSGSEAERLRFWAGR
                     KAAFPAVGRISPDYYCMDGTIPRRRLGDVLLFIEELQAKYGLRCPNVFHAGDGNLHPL
                     ILYDANDPDQLARTERFAGEVLEKCVEVGGTITGEHGVGVEKINQMCVQFGEREIAAF
                     QGVKRAFDPQGLLNPGKNIPSLHRCAEFGRMRVHGGALPHPDLPRF"
     gene            6086..6862
                     /locus_tag="VI319_07125"
     CDS             6086..6862
                     /locus_tag="VI319_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863658.1"
                     /translation="MRRLSRPATLVAALLALASPPALAIVDEIQVYTDEVDARGESGL
                     ELHINTTPRGRKTPDYEGDVPPYRSTRITPEFSWGLGHDMDWGLYLPTVVDRDGHWVA
                     SGAKLRFKWMPLHPAEDAEGWYAGANIELSRLSKKYSDSPWSAELRLITGYHARDWLV
                     GVNAIPEWGLSKGFRGSPDFELAWRAVHRAAGPFAMGLEYYNGYGTLADRLPGPEQDR
                     TLYVVGEYEAKSWSLSLGVGHGLTPASDNVTVKAIVGIEF"
     gene            6864..>7295
                     /locus_tag="VI319_07130"
     CDS             6864..>7295
                     /locus_tag="VI319_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY6863659.1"
                     /translation="MDAILDEWSAKIRDAQAARRPLRLRGGGTKDFYGHALDGEVLDT
                     RGYAGIVAYEPSELVVTARCGTPLAQVEHALAGRGQMLAFEPPRYAAGGTLGGMLAAG
                     LSGPRRQACGAVRDFVLGVRVLDGRGADLRFGGQVMKNVAGY"
BASE COUNT         1043 a         2599 c         2592 g         1061 t
ORIGIN      
        1 gatcgatccc gacctccaca agctcttccc tgactcggag gccgtaaatc gcgcgctgcg
       61 tgactacgcc tcgaaaaagc gaacggcaac ctagcggcgc gagctagatc gccccgctct
      121 tcttcagcag cgccgccacc ccttctgcca tcttcgcgta ccccttcgcg ttcgggtgca
      181 cgaggtcgga cttgagctcg ttgtcggtga ggatcttgac gagcgtcgag ttgtcgtagg
      241 ggatcttcat ctcggtcgcg atgtcggtgt agagcgtcgc ggtcgacggc atcaagcccg
      301 gcttcggcac gccgatcagg acgacctggg cgccttgcgc gcgcgcgagg ccgatcatgg
      361 agcgcaggtt ctgggcggcg atcttttcgt cgaggtgctg gaggaaatcg ttgccgcctt
      421 cgcacaggat gagaagggcc ggcttggatt cgtcgagcgc ggattgcagc cgcgcgaggc
      481 cgccggcggt ggtctcgccg gggattccgg ccgagacgac cttgcggccg atcagcgcct
      541 gaaggcgcgc ggggtagctc tcgttcgggt tggcgccgta gccgtaggtc aggctgtcgc
      601 cgaaggcgag gacgacggcg tcgcccggga gcttcgcgag cttgggcgag ctgccgccgc
      661 cgcacgcggc gagggcctcg acgatgaaaa gcgcgacgag gaccagcgcg gcgcgcttca
      721 cgacgggtct ttcggcggcg gcagggtctt gcactcgacg aagatgcgcg cgcggcacgt
      781 cgcgcagacg tcgcggtcca ggcgctcgaa gatcgtggcg aacgcgtctt gcttgccgcg
      841 gaagacgttc tcctcgccga tccgcttcat gtagccgccg cgccggagca ggtcctcgac
      901 gggctggcgc aggctgtaga agtacaggcg cccgccgatc gcgcgccttc gctcggcctc
      961 ctcggcgagg agctcggcgc ccgccatgtc gacgaagttg acgctcttcg ccatgaggat
     1021 gaggtgctcc tgcgatttcg cgtgctcgcg cagggtgtcg aagtggcgct cgacgtggct
     1081 cacggcgccg aaccagatcg agccttcgat cctcaggatc ttcgcctgcg ggcactcgcg
     1141 caggtcgtcc tcgggctcgg tcatcttgcg attcgggtgg cgcgggtcgg ggacgagggt
     1201 gcgcatgatc gggtgcgagg tgcggtgcag gtagacgacg agcgacgcga gcacgccgag
     1261 cgcgatggcg agctccatgt cgaagagcag cgtcgccgcg aaggtgatga cgagcacggt
     1321 ggtctcgccg cggctggtgc gcacgatctg gcggatgcgc gcgaagtcga tcaggctcca
     1381 ggcgacgatg aacagcatcc ccgccacgac cgcgtacggc aggtacgccg cgaggcctcc
     1441 gaccgcggcg aggacgagca cgaggaacag cgccgagagg atcgccgcca tcggcgtgcg
     1501 cgcgccgctc tcgtaattga gcacgcagcg gttcaccgac atgctcaccg ccatccccga
     1561 gaagaagctc gccgagacgt tggcgagccc ctgtccgacg aactcctggt tgtagtcgac
     1621 gcgctgcccg gacttgagcg cgagcgcgcg cgtcaccgag taggcctgga tcaggcacac
     1681 ggccgcgacc gcgagcgcgg gaccgagcag cgtgcgcagc gtgagcggcg agacgtcggg
     1741 caccgacagc ggcgagagcg cgccgggcag cgccccgagc gtgcgcaggc cggtgcgcgc
     1801 cggccccagc caggcgttga gcgcccaggc gagcgcgccg cccgccagca tcgcgaccag
     1861 catcggcggc acgcgcggga aaaattcgcg ggcgagcgcg ccgacggcga gcgtcgcgca
     1921 ggccacgacc acgacccagg gctggatgtg cgagagccga atgaacgtcg agagcacgag
     1981 cgccgtgaag ctcgacgccg ccgggatccg gatcccgagg aaatccggca gctggctcgc
     2041 gaacacgagc agcgccaccg ccgccgtgaa cccgaccacc accgtgtccg acacgaactg
     2101 cagcagcgac ccgagcctca gcagcccgag cgcgatcatg atcgcacccg ccatgagcgc
     2161 gagcgtgagc gcgaggtgga tgtagtaggg cgtgcccggc accgcgaggc ccgcgagcga
     2221 cgcgagcacc atcagcgaca cggggttggt cggccccgac atcccgtgca gcgacgagcc
     2281 gaacagcgcc gcgatcacca ccggcaccat cgaggcgtag aggccgtact cgggcggcag
     2341 tcccgcgagc gtggcgaacg cgacgccctg gggcagcacc accgtcgcgc cgacgaagcc
     2401 ggcgatcacg tccgcgcgca gcgtccggcg gtcgacgagc ggccaccact tcaggaacgg
     2461 aagcgtccga aggaaaaccg ggcgggcgac gcgggaatcc atccggctca atggtagcaa
     2521 accgcgtgct atgctcgcgt tcttccgcgc agagccgcag aggacttccg ccttgtcccc
     2581 ccgcaatctg accgtgatgc tcgtctgctc gagcctgatc ctcacgctcg cgctcggcat
     2641 ccggcacagc ttcggcctgt tcctcacgcc catgagcctc gagcacggct gggggcgcga
     2701 ggtcttcggc ctcgcgatcg cgatccagaa cctgctctgg ggactgtcgc agccgctcac
     2761 cggcatgctc gccgaccgct acggcgcgcc gcgcgtgctg atggcgggcg gcgtgctcta
     2821 cgccgcgggc ctcgtggcga tggcgtacgc gcccacgccg ctcgtcttca cgctgtccgc
     2881 gggcctgctg atcggcttcg gcatgagcag caccaccttc aacgtcgtgt tcggcgcgct
     2941 cgggcgcgcg ttcccgcccg agcagcgctc gaagatcctg gggctcggca gctcggccgg
     3001 atcgttcggg cagttcgtca tgctgccgct cgcgctgttc ctgatctcgg cgctcgggtg
     3061 gcactgggcg atgctcgccc tcgcgctcac cgcggcgacc atgatccccg cggcgctcgg
     3121 cgtgcccgac cagggctacg gcggcgcatc gtcggcgggc gggctgccgc tcaaggccgc
     3181 gctcggcgag gcgacgcgcc accgcggctt ctggctgctc ggcgtcggct acttcacctg
     3241 cggcttccag atcgtcttca tcggcacgca ctttccgtcg ttcctgctcg accacggcct
     3301 cagcgcgcgc gacggcaccg tggcgctcgc gctcatcgga ctgttcaacg tcttcggctc
     3361 gtacctcgcg ggctacctgg gcggacgctt tccgaagaac tacctgctct ccggcctgta
     3421 cgccgtgcgc ggcctggcga tcgcgctgct gctcgccttt ccgctgacgc cgttcagcgt
     3481 ctattgcttc gccgcgggct tcggcttcac ctggctcggc accgtcccgc tcaccaacgg
     3541 cgtcgtcgcc ggcatcttcg gcgtgcgcca cttcgcgatg ctctcgggca tcgtgttcat
     3601 gttccaccag ctcggcagct tcttcggcag ctggctcggc ggctggatct acgaccatac
     3661 cgggtcctac aacgccgttt ggatgatcgc gatcgggctg tcggtcgtgt cgaccctcgt
     3721 caacctgccg gtcgaggaga agccggtcgt ccgcgggccc gcggcggcct gagccggcat
     3781 gccgcggttc tgcgccaaca tcagcatgct gttcgccgag cgcgagttcc ccgagcgctt
     3841 cgacgccgcg gcgcgctcgg gcttcgccgc cgtcgagatc cagttcccct atcgctggag
     3901 cagggaagcg ctcgccgagc gcgcccgcgc cgccggcgtc gagatcgtgc aaatcaacct
     3961 gccggcgggc gacgcgcagc gcggcgaccg cggcatcgcc tgcgatccct cgcgcgcgga
     4021 ggaattccgc gacggcgtcg gccgcgcgct cgagtacgcg cgcgcgctcg gctgccgcca
     4081 gatgaactgc ctcgccggga tcgcgccgcc gggactgccc gaggcggcgc tgcgcgagac
     4141 cttcgtcggc cacttgcgct gggccgcggc cgcgctcgcg cgcgagggga tgacgctcct
     4201 ggtcgagccg atcaacacgc gcaccatccc ggggttctgg ctgaaccggc cgtcgcaggc
     4261 gctcgcgctc atcgacgagg ccggcgcggc gaacctgaag ctgcagttcg acgtcttcca
     4321 cgtccacgtc atggagggcg acgtcggccc cgcgctcgag gcggccttcg cccatatcgg
     4381 ccacatccag gtcgccgacg acccgggacg gcacgagccc ggcaccggcg cgatcgattt
     4441 ccccgcgctg ttcgagcgca tcgacggcct cggctaccgc ggctggatcg gcgccgagta
     4501 cgtgccgtcg gcggcgaccg aggatacgct cggctgggcg cggcgctggg tgcacgaggt
     4561 tcccgagcgc cgcaatccgt ggtagaaaat ccgccatgaa cgtcgacgac ctgcaccagc
     4621 cgatcgacgg cgcgcgccag gccgaggtgg tcgcgaggct gcgcgcgatc ctgccgcccg
     4681 cggcgctgct ccacgccgcc gaggacacga agagctacga gagcgacggc ctgtccgcgt
     4741 accggcagcc gccgatggtg gtcgcgctgc ccgacaacga ggaccaggtg gccgcggtgc
     4801 tgcgcgcgtg ccgcgacctc ggcgtccccg tggtcgcgcg cggctcgggc accgggctct
     4861 cgggcggggc gctgccgcgc ggcgacggcg tgctgctctc gctcgcgaag atgcgccggg
     4921 tgctcgcggt cgacccggtc gcgcgcgtcg cgcgcgtgca gccgggcgtc accaacctcg
     4981 cggtgtcggc ggccgccgag ccctacggcc tgttctacgc gcccgatccc tcgtcgcaga
     5041 tcgcgtgctc gatcggcggc aacgtcgccg agaactcggg tggcgtgcac tgcgtgaagt
     5101 acggcctgac cgtgcacaac gtgctgcgcg cgcgcgcggt cacgatcgag ggcgaggtca
     5161 tcgagctcgg gtcggaggcg cccgacgcgc cgggcgtcga cctgctcgcg ctcctgatcg
     5221 gcagcgaggg catgctcgcg gtggtcaccg aggtcacggt caagctgctg ccgaggcccg
     5281 agacggcgcg ctgcgtgctc gcctcgttcg acgacctgga gaaggcgggc aacgccgtcg
     5341 ccgacatcat cgcggccggc atcatccccg cgggcctgga gatgatggac cggccggcga
     5401 cgcgcgcggt cgaggacttc gtacacgcgg gctacgacct cgaggcgaag gcgctgctcc
     5461 tgtgcgagtc ggacggcacg cccgaggagg tcgaggagga ggtcgcgcgc gtgaccgcgg
     5521 tgctcgagcg cgccggggcg acgcgcttcc gcgtctcggg ctcggaggcc gagcgcctgc
     5581 gcttctgggc cgggcgcaag gccgcgttcc ccgcggtcgg ccgcatctcg cccgactact
     5641 actgcatgga cggcaccatc ccgcggcgca ggctcggcga cgtgctgctc ttcatcgagg
     5701 agctgcaggc gaaatacggg ctgcgctgcc cgaacgtctt ccacgcgggc gacggcaacc
     5761 tgcacccgct catcctgtac gacgcgaacg accccgacca gctcgcgcgc accgagcgct
     5821 tcgccggcga ggtgctcgag aaatgcgtcg aggtcggcgg caccatcacc ggggagcacg
     5881 gcgtcggcgt cgagaagatc aaccagatgt gcgtgcagtt cggcgagcgc gagatcgccg
     5941 ccttccaggg cgtgaagcgc gccttcgatc cccagggcct gctcaacccc ggcaagaaca
     6001 tcccgtcgct gcaccgctgc gccgagttcg gccgcatgcg cgtgcacggc ggcgccttgc
     6061 cgcatcccga tctgccgcgg ttctagtgcg aaggctctcc cgtcccgcga cgctcgttgc
     6121 cgcgctcctg gcgctggcgt ccccgcccgc gctcgcgatc gtcgacgaga tccaggtcta
     6181 caccgacgag gtcgacgcgc gcggcgagag cggcctcgag ctgcacatca acacgacgcc
     6241 gcgcggccgc aagacgcccg attacgaggg tgacgtgccg ccgtaccgct cgacgcgcat
     6301 cacgcccgaa ttctcctggg gcctcggcca cgacatggac tgggggctgt acctgccgac
     6361 cgtcgtcgac cgcgacggcc actgggtcgc ctcgggggcg aagctgcgct tcaagtggat
     6421 gccgctgcac cccgccgagg acgcggaagg ctggtacgcg ggcgcgaaca tcgagctctc
     6481 gcgcctatcg aagaagtact ccgactcgcc gtggagcgcg gagctccgcc tcatcacggg
     6541 ataccacgcg cgcgactggc tggtcggcgt gaatgcgata cccgagtggg ggctgtcgaa
     6601 gggcttccgc ggctcgcccg acttcgagct ggcgtggcgg gcggtgcacc gcgccgccgg
     6661 tcccttcgcg atggggctcg agtactacaa cggctacggc acgctcgccg atcgcctgcc
     6721 cggtcccgag caggaccgca cgctctacgt cgtcggcgaa tacgaggcga aatcgtggtc
     6781 gctcagcctc ggcgtcggcc acggcctcac ccccgcgagc gacaacgtga cggtcaaggc
     6841 gatcgtcggg atcgagttct gagatggacg cgatcctcga cgaatggtcg gcgaagatcc
     6901 gcgacgcgca agccgcgagg cggccgctgc gcctgcgcgg cggcggcacc aaggacttct
     6961 acggccacgc gctcgacggc gaggtcctcg acacgcgcgg ctacgccggc atcgtcgcct
     7021 acgagccgag cgagctggtg gtcacggcgc gctgcggcac gccgctcgcg caggtcgagc
     7081 acgcgctcgc cggccgcggc cagatgctcg cgttcgagcc gccgcgctat gccgccgggg
     7141 ggacgctcgg cggcatgctc gccgcgggcc tctcggggcc gcggcgccag gcgtgcggcg
     7201 cggtgcgcga cttcgtgctc ggcgtccggg tgctcgacgg ccgcggcgcc gacctgcgct
     7261 tcggcgggca ggtgatgaag aacgtcgccg gctac
//
LOCUS       DATDMW010000165         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_49093, whole genome shotgun sequence.
ACCESSION   DATDMW010000165 DATDMW010000000
VERSION     DATDMW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49093"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VI319_07135"
     CDS             <1..615
                     /locus_tag="VI319_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6863660.1"
                     /translation="MALVPSVLVVNPRVLDVKSAQELIAFARAHPGKLTYGSAGLGST
                     SHLTTEYFKLATGTDILHVPYKGVGPVMTDLVGGQISMGLNGAPAVMPYVSQGRLRAL
                     AVSSAKRLEALPDLPTLAESGVPGFDANGWYGIVAPAGTPRVAVDRLNAEVRSSVATP
                     ELKKRLDTEGAIAAAGTPEEFGAFIVSEIERWGAVLRKAGIQPQ"
     gene            complement(616..1212)
                     /locus_tag="VI319_07140"
     CDS             complement(616..1212)
                     /locus_tag="VI319_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY6863661.1"
                     /translation="MSRRLFAFLSTLLAAFAVVAGHPAHAADDDAVLLVAQPKLVDAV
                     YNKTVLIVKPLPNGGHAGFIVNKPTEAHLGEVFPDHAPSKKVVDPLYLGGPESLNTVF
                     ALVERQKAVDESEVPFSPGIYLAVKSTDVDHVIEAESDHARFLVGLVIWKPGELDAEL
                     EKGFWYQMDADPKVFMRKDTTHLWEELMQKGEDRKHAT"
     gene            1522..1881
                     /locus_tag="VI319_07145"
     CDS             1522..1881
                     /locus_tag="VI319_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863662.1"
                     /translation="MNITSEYLADLLIGIARAQNAIVEAIERAEPGFRSTHLLPVLTV
                     AANMRTGDPRAIDLASRILLRLQGRVALDNAQVKADIERLATEVKKDAVAFTPPQAPR
                     AAGPATQPGSDLDFSTK"
BASE COUNT          367 a          687 c          712 g          353 t
ORIGIN      
        1 atggcgctcg tgccctcggt gctcgtggtc aatccgcggg tgctcgacgt gaagagcgcg
       61 caggagctga tcgcgttcgc gcgcgcgcac ccgggcaagc tcacctacgg ctcggcgggg
      121 ctcgggtcga cctcgcacct cacgaccgag tacttcaagc tcgcgaccgg caccgacatc
      181 ctgcacgtgc cgtacaaggg cgtcgggccg gtgatgaccg acctcgtcgg cgggcagatc
      241 tcgatgggcc tgaacggcgc gccggcggtg atgccctacg tgagccaggg acgcctgcgc
      301 gcgctcgcgg tctcgagcgc gaagcggctg gaagccctgc ccgacctgcc gacgctcgcc
      361 gagtcgggcg tgcccgggtt cgacgcgaac ggctggtacg ggatcgtggc gccggcgggg
      421 actcctcgcg tcgcggtgga ccgcctgaac gccgaggtgc gcagcagcgt cgccacgccg
      481 gaattgaaga agcgcctcga caccgaaggc gcgatcgccg ccgccggcac tcccgaggag
      541 ttcggcgcgt tcatcgtgtc ggagatcgag cgctgggggg cggtgctcag gaaggccggg
      601 atccagcccc agtgatcagg tcgcgtgctt ccggtcctcg cccttctgca tcagctcctc
      661 ccacaggtgc gtcgtgtcct tcctcatgaa caccttggga tccgcgtcca tctggtacca
      721 gaagcccttc tccagctcgg cgtcgagctc gcccggcttc cagatcacca ggcccacgag
      781 gaagcgcgcg tggtcggact cggcctcgat cacgtggtcg acgtcggtgc tcttcaccgc
      841 gaggtagatg cccggcgaga acggcacctc gctctcgtcg accgccttct ggcgctcgac
      901 cagcgcgaac accgtgttca ggctctcggg cccgcccagg tagagcgggt cgacgacctt
      961 cttcgacggc gcgtggtccg ggaagacctc gcccaggtgc gcctcggtcg gcttgttcac
     1021 gatgaagccc gcgtgcccgc cgttggggag cggcttcacg atcaggaccg tcttgttgta
     1081 gacggcgtcg accagcttcg gctgggcgac gagcaggacc gcgtcgtcgt cggcggcgtg
     1141 cgcggggtgg cccgcgacga cggcaaaagc tgcaagcagc gtggacagaa atgcaaacaa
     1201 gcgacgcgac atcggaatct ccaggcccgt gaaacaccgt accacggccg ccgcgcggcc
     1261 gctcgaagcg gtgcgatgca acgagcgtgc ccgtcgattg ttacagttaa cgcatcgcgc
     1321 cggtgacacc ttcgcgaagg gcggagggca aagcgcacga cagggtaatg tcagactgta
     1381 ggggttttcc cggcgtgacg gatagacggc agccatgtca tcctgagcgc agcgaaggat
     1441 ctgcttttgc cggaacaggc gccccagacg ccacctggtt tatccttcac cgcgaatgca
     1501 acaagaccca gggagggtcc cgtgaacatc acctccgagt acctcgccga cctcctcatc
     1561 ggcatcgccc gcgcgcagaa cgccatcgtc gaagccatcg agcgggcgga gcccgggttt
     1621 cgcagcaccc acctgctgcc cgtcctcacc gtcgccgcga acatgcgcac cggcgatccg
     1681 cgcgcgatcg atctcgcgtc gcggatattg ctgcggctgc aggggagggt cgcgctggat
     1741 aacgcgcagg tgaaggcgga tatcgagcgg ctggcgacgg aggtgaagaa ggacgcggtg
     1801 gcgttcacgc cgcctcaggc gccccgggcg gcggggccgg cgacgcagcc ggggagtgat
     1861 ttggatttca gcacgaaata gaacacttag aatccgatgc caacgaagcg cagatccaag
     1921 agaggactgc ttcttcagca attggaagac gtgtcttggc gtgtcttcga gacttatgga
     1981 gatgtgctac gcgatttgat tcgcggtcgc ccgggcgtct atgtgctgta ccgcaagcag
     2041 cgggtttact acatcggtct ggccagcaat ctcatgggac gactaaatac tcatctaagc
     2101 gattgtcatc atggtcttt
//
LOCUS       DATDMW010000166        14061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1916614, whole genome shotgun sequence.
ACCESSION   DATDMW010000166 DATDMW010000000
VERSION     DATDMW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14061
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916614"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VI319_07150"
     CDS             <1..1330
                     /locus_tag="VI319_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY6863663.1"
                     /translation="LVMGALSMGLPTIYMPAGPMLRGNWHGQVLGSGSDTRKYWAELR
                     AGTITEHDWEEIEEGIARSPGHCMTMGTASTMTSAVETLGLALPGSASIPAADSHHAK
                     MATATGKRAVEMVWEDVTPKSVLTAASFDNAVTCVLACGGSTNAVIHLIAMARRAGVA
                     LDLQRFDALSRKVPVIANLRPAGKYLMEDFYYAGGLPALLASISDLLDLSAKTITGGT
                     LGANIAGAKNFNADVILPREKALIASGGLAVLRGNLAPDGAIVKPAAAEKRLLQHKGR
                     AVVFEDYDDLQKRIDDPALDVDADCVIVLKNAGPVGAPGMPEWEVPIPKKLLQQGVRD
                     MVRISDARMSGTAYGCCVLHVAPESWVGGPLALVKDGDVIELDVPGRRLQMHVSDAEL
                     EKRRKAWRAPARRFERGYGALFSQHIRQANEGCDFDFLEGTAPTPEPEIH"
     gene            complement(1327..2892)
                     /locus_tag="VI319_07155"
     CDS             complement(1327..2892)
                     /locus_tag="VI319_07155"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY6863664.1"
                     /translation="MRALLLCFALVSAGAAGAGADRGELAGTIRDAAGKPLTGAAVKI
                     RNLDRGVTVTVFSRAGRYRAAGLFPGKTEVSAGPDGHAPGAERTIEIATGKAAKLDLA
                     LRASDPVVTTADWIPQLPDDGDGAKQLVVNRCVNCHGPVNFVSRRFDQLGWKKVILDM
                     GRIEETRKARWREIPDEQAMAAKGEEIERLSRYLARHYGPDKAPQVQAPKPVAYRSTR
                     GATDIVITQFDIPTRGAVPHNVASDAAGRVWFVERFADKVGMLDPQTAGFRELPIPTG
                     ARPHGVIADAGGIVWWTESRGNHLGRLDPRSGEMKRYPLPHERSGPHSLALARDGAIW
                     LTEIGGNRIARFDPAAGRFTEHEVPTKRSRPYGIAIDARGDVWFCEFTGDRIGRLDPA
                     TGRITEYAPPTPRSGPRRLRIDAEGGVWFTEYNTNRIARLEPATGAMREWTIPTPDSG
                     PYDIAVDARGMVWFDEFTANKIVRFDPATARFDEFPLPGPDSQVRKMAFDPSGALWLS
                     EYTGSRMVRVVER"
     gene            3005..3790
                     /gene="hpaH"
                     /locus_tag="VI319_07160"
     CDS             3005..3790
                     /gene="hpaH"
                     /locus_tag="VI319_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HEY6863665.1"
                     /translation="MALSEADRKKAVEILLNAEKEKKQATQLSVTFPGITIEDAYAIS
                     TAVAQHKIKGGAKLIGHKVGLTSKAMQASSQINEPDYGHLLDYMMIPDGAKVPHANYC
                     VPRVEVELAFVLGKALKGPGVGLLDVLRATEYVIPAMEIVDARIVNPRKIFDTVADNG
                     AAAGIVCGGRPIKPGDVDLRWVGGIMYRNSEIEETGLAAGVLGHPALGVAWLANRLGQ
                     NGVTLEAGHLVLAGSFTRVVFAQKGDTLHGDFGPLGGVAVQFV"
     gene            3805..4479
                     /locus_tag="VI319_07165"
     CDS             3805..4479
                     /locus_tag="VI319_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY6863666.1"
                     /translation="MAKVGFRILPVAKRPDKKLLAELAKMVTPHLSDSMERLYAGGAQ
                     LRPMHDGTKLCGPAFTVRTSPGDNLLVHKAIDIAEPGDVIVVDAGGVCDNAIIGELMS
                     ARAEQRGVAGMVIWGAIRDSKELREGSFPVYASGVTHRGPYKNGPGEINVAISIGGMT
                     VNPGDVIVGDADGLVAIPQDQAEAVLASAKAILAKEEKSMKEIKAGTVDRSWVDKALK
                     EKGYQV"
     gene            4482..5246
                     /locus_tag="VI319_07170"
     CDS             4482..5246
                     /locus_tag="VI319_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEY6863667.1"
                     /translation="MDLPVNTFKRALKAGKPQIGLWCSLSSHYAIELVAGSGFDWLLL
                     DTEHSPNDLESVLTQLQAAAPYPTHPVVRVPWNDMVTIKRFLDIGSQTILIPYVQNAE
                     EAKAAVAHTRYPPAGVRGVAGGTRATRWGRVKDYAKRAHEEICVLVQVETQTALDNLE
                     RIAAVDGVDGVFIGPADLHASLGYPGETNNPKVVPVIDDAVRRIRKAGKAPGILTTVE
                     ADAHRWLECGGQFVAVGVDTVVLARGTEALAAKFKT"
     gene            complement(5243..5695)
                     /locus_tag="VI319_07175"
     CDS             complement(5243..5695)
                     /locus_tag="VI319_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEY6863668.1"
                     /translation="MDSKRTERLRKAMDGLAGSVGHFLVDAFHYLALFAIGGAIVWSA
                     ISSFWGMAAKGGASIDDILLLFIYLELGAMVGIYFKTNHMPVRFLIYVAITALTRMMI
                     GDVQEKHLHPDMGIILIAGAILLLALAILVIRYGSNRFPSGDGRDTTS"
     gene            5741..6574
                     /locus_tag="VI319_07180"
     CDS             5741..6574
                     /locus_tag="VI319_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6863669.1"
                     /translation="MKTVKLTAGDPVPALGLGTWRYGEDRSRRTAEIATLKLALDLGA
                     TLIDTAEMYGEGGAEEVIGEAIAGRRDEAFLVSKVYPHNATRKGTAAACERSLKRLKT
                     DRIDLYLLHWRQGTPLGESVEALAALERAGKIRHWGVSNLDLSDMRELWELADGRGVQ
                     TNQLLYNLSRRGIEWDLMPWLRERRIPVMAYSPIEQARLVSHRGLADFAKRHGATPAQ
                     AALAWLLAKDDVIVIPKTSRVDRLKENLGALEIRFSKDQLAELDRLFPQPKGPQPLEM
                     L"
     gene            complement(6571..7614)
                     /locus_tag="VI319_07185"
     CDS             complement(6571..7614)
                     /locus_tag="VI319_07185"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY6863670.1"
                     /translation="MATLQSLLGIDLPIVQAPMAGVQLSALAVAVSNAGGLGSLPCAM
                     LAPEAMRKELSALRAGTTKPYNVNFFCHAQPAPSAEREAAWRKALAPYFAEYGIDPAT
                     IPSGPGRLPFSAEAADVLEEFRPPVVSFHFGLPSKELLARVRAFGAKVLGSATTVDEA
                     RWLEARGVDAVIAQGVEAGGHRGNFLSDDLTAQLGTFTLVPQVVRAVKVPVIAAGGIA
                     DAKGVAAAIALGAAGAQVGTAYLLCPEATTSALHRAALKSEAARHTALTNLLTGRPAR
                     GIVNRVMKELGPLSTLAPAFPLATAAIMALRAKAEAQGRTDFSSLWSGQNASGCKEIP
                     AAQLTRELSANLS"
     gene            complement(7863..8234)
                     /locus_tag="VI319_07190"
     CDS             complement(7863..8234)
                     /locus_tag="VI319_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863671.1"
                     /translation="MKLFLSIAGALAGLFGAMLLFVPAKFYEPTGMVLTPMLATLAQA
                     HGATLVGMGTVLWLARSATGQGLIAVLAGNLVVQILSLAVVVRTMTLGAGAAVAPGIV
                     IHVVLGALFLFFLLKARRVGR"
     gene            9041..10102
                     /locus_tag="VI319_07195"
     CDS             9041..10102
                     /locus_tag="VI319_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY6863672.1"
                     /translation="MNVPAPQLSEQVDAIFSAWGMSEPAREACVRLMIAADLRGIDSH
                     GVMMLPLYDELRQDGRLKMEPNVRTVRESPVTALVDGDMSLGHYPADYAMKLAIRKAR
                     GSGLAAVAVRNSNHFGAAGVYAMQAASAGLIGIAMTNVWNLAIVPTRAAQAMFGTNPI
                     AFAAPAGEQPPFCLDMATSTVAIGKIKLAILHGKPLPHGWAHAADGRITADANAALAA
                     RLLTPVGGTPELSSHKGYGLAAMVEILCAMLPGAWYAPTRDQRHPGEKRYNVGHFFLA
                     MDPNAFRAEGEFEADLDDMIGALRSAKRLDPAEPVLVHGDPERAEMERRGREGVPVPD
                     ALATKIRSIAADCGAPALI"
     gene            10226..10630
                     /locus_tag="VI319_07200"
     CDS             10226..10630
                     /locus_tag="VI319_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY6863673.1"
                     /translation="MRLAPRPAILAALACTLALSLSACGEHSTPATKSTDVTPPQPAQ
                     QTVPAAPAPAPAAQAPKADPDAELAARVKSAFASDPQLKMLPIDVRASNGTVTLYGTA
                     DSARLRDKAIHTAEAVDGVKSVSANLQIVRGS"
     gene            complement(10638..11597)
                     /locus_tag="VI319_07205"
     CDS             complement(10638..11597)
                     /locus_tag="VI319_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863674.1"
                     /translation="MRLLVPLVVFTLAWLSVARAEYPEHPVRLIVPQAAGSATDTIAR
                     LLVAELPAQLGQQVVVENRPGGALTIGINLVAKAAPDGYTVGMGPIGAMAITRHMVSK
                     LPYDIERDLQPIALVVRSHLLLAVSPSLPVHSVRELIAYAKENPGKLLNASSSNGSPG
                     HVGGELFKTMTGTDIVHVPYKGGSMAINDLIAGHVQVMFESLNSIAPYARSGRVRALA
                     VSGAHRSAGFPDLPTIAEAGVPGYEAGTWTGVIGPAGLPRPVVLRLNAAINRAILTPA
                     SKKRFDDIGNEPGGGTPEEFGELIRRESAKWGEVVKRSGAKID"
     gene            11645..12049
                     /locus_tag="VI319_07210"
     CDS             11645..12049
                     /locus_tag="VI319_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY6863675.1"
                     /translation="MPKQIITSPDAPVTGFTDKGTPSPLAQAIRAGNLLFVSGQGPLE
                     PKTREVVAGDIRAQTRQTLTNLKAVLAAAGLDFRHVVNMRVVLRNPADFPAFNETFRD
                     FLQGEKVTRTCIGATPHRKGVDVEIDCVASYD"
     gene            complement(12085..12660)
                     /locus_tag="VI319_07215"
     CDS             complement(12085..12660)
                     /locus_tag="VI319_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863676.1"
                     /translation="MRKISAPAVAFAALALWGVATATAAVRDDVDRLGARRDRNDSRE
                     VRSTLPRNAAGALDVPRLIVEIRAALTRGERDIRFRDTALSAGEANKLRELAARFGLE
                     PVRIGDEGRHAVRVEFRDVDNSDRDRAGPRPDAREDAITHVELRERSPRPERAEKPDR
                     PDKPERADKPERAEKVERADRPERGDRSGRH"
     gene            complement(12806..13654)
                     /locus_tag="VI319_07220"
     CDS             complement(12806..13654)
                     /locus_tag="VI319_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863677.1"
                     /translation="MWTKHLFVTAAALAVLAPAGFAQAQSSIDVRQEDRRVDRQDDRR
                     MDRRNDATESEVENENQNERAEVKLAVPRTATGAVDVDKLLALVRDQIAAGAREIQFR
                     GVTLTAADVQGLLLSTAASDNLLAEIAALLPKDGLERKVTLRGAVDVRVTRQPDGTLR
                     ARIEDVNLAGLTPEQRAQLAQQLAALGFTRVEIRGVDSAGKRVRVEFRAARGVRNDDR
                     GTSSDNRGRRDDRVADARGDRRAADDRPQRAERPERAERAERPERVERVEQVERVDRP
                     DRSGRH"
     gene            complement(13751..>14061)
                     /locus_tag="VI319_07225"
     CDS             complement(13751..>14061)
                     /locus_tag="VI319_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863678.1"
                     /translation="WQKPLMAAVAIASLGLLGAQEVPAQTADANDVRQEDRQQDRQAD
                     RQQDRRADRQQDRRMDRRDDAMDRPERIERAERAERPERAERTERAERVESPDRSGRR
                     "
BASE COUNT         2164 a         4947 c         4864 g         2086 t
ORIGIN      
        1 gctcgtgatg ggcgcgctgt cgatggggct gccgaccatc tacatgcccg cgggtccgat
       61 gctgcgcggc aactggcacg gacaggtcct cggctcgggc agcgacacgc gcaaatactg
      121 ggcggagctg cgcgccggca ccatcaccga gcacgactgg gaggagatcg aggaaggcat
      181 cgcgcgctcg cccggccatt gcatgaccat gggcacggcg tcgacgatga cctcggcggt
      241 ggagacgctc gggctcgcgc ttcccggctc cgcctcgatc cccgcggcgg attcacatca
      301 cgccaagatg gcgaccgcga cgggcaagcg cgcggtggag atggtgtggg aggacgtcac
      361 cccgaagagc gtcctcacgg ccgcgtcctt cgacaacgcg gtgacgtgcg tgctcgcctg
      421 cggcggctcg actaacgccg tcatccatct catcgcgatg gcgcggcgcg cgggtgtcgc
      481 gctcgacctg cagcgcttcg acgcgctatc gcgcaaggtg ccggtgatcg ccaatctccg
      541 gccggcgggc aagtacctga tggaagactt ctactacgcc ggcggccttc ccgcgctgct
      601 cgcgtcgatc tccgatctgc tggatctgtc ggcgaagacc atcaccggcg ggacgctcgg
      661 cgcgaacatc gccggcgcga agaatttcaa tgccgacgtc atcctgccgc gcgagaaggc
      721 gctgatcgca tcgggcggcc tcgcggtgct gcgcgggaac ctcgcgcccg acggcgcgat
      781 cgtgaaaccg gcggcggcgg agaagcgtct gctgcagcac aagggccgcg ccgtggtgtt
      841 cgaggattac gacgacctgc agaagcgcat cgacgaccct gcgctcgacg tcgacgccga
      901 ctgcgtgatc gtgctgaaga acgccggccc cgtcggcgcg cccggcatgc ccgaatggga
      961 ggtgccgatc ccgaagaagc tgctgcagca gggcgtgcgc gacatggtgc ggatctccga
     1021 cgcgcgcatg agcgggaccg cctacggctg ctgcgtgctg cacgtcgccc ccgagtcgtg
     1081 ggtcggcggg ccgctcgcgc tggtgaagga cggcgacgtg atcgagctcg acgtccccgg
     1141 ccggcgcctg cagatgcacg tctccgacgc ggagctggag aagcgccgca aggcgtggcg
     1201 cgcgcccgcg cgccgcttcg agcgcggcta cggcgcgctc ttctcgcagc atatccgcca
     1261 ggcgaacgag ggctgcgatt tcgacttcct cgaaggcacg gcgccgacgc ccgagcccga
     1321 gatccactag cgctcgacca ctcgcaccat gcggctcccg gtgtactccg agagccacag
     1381 ggcgcccgac ggatcgaacg ccatcttgcg cacctgcgag tcggggcccg gcagcggaaa
     1441 ttcgtcgaag cgggcggtcg cgggatcgaa gcgaacgatc ttgttcgcgg tgaactcgtc
     1501 gaaccagacc atgccgcggg catcgacggc gatgtcgtaa gggccggaat ccggcgtcgg
     1561 gatcgtccac tcccgcatcg cgccggtcgc gggttcgagg cgcgcgatcc ggttggtgtt
     1621 gtactcggtg aaccacaccc cgccctcggc gtcgatccgc agccggcgcg gtcctgagcg
     1681 cggcgtcggg ggcgcgtact cggtgatcct cccggtcgcg ggatcgagcc tgccgatcct
     1741 gtcgcctgtg aactcgcaga accagacgtc gccgcgcgcg tcgatcgcga tgccgtacgg
     1801 gcgcgagcgc ttcgtcggca cctcgtgctc ggtgaagcgc ccggcggccg ggtcgaagcg
     1861 cgcgatgcgg ttgccgccga tctcggtgag ccagatcgcg ccgtcgcgcg cgagcgcgag
     1921 gctgtgcggg cccgagcgct cgtgcggcag cggatagcgc ttcatctcgc ctgagcgcgg
     1981 gtcgaggcgc ccgagatggt tgccgcggct ctcggtccac cagacgatgc cgcccgcgtc
     2041 cgcgatcacg ccgtgcggac gcgcgcccgt cgggatgggc agctcccgga aaccggccgt
     2101 ttgcgggtcc agcatgccga ccttgtcggc gaagcgctcg acgaaccaga ctcggcccgc
     2161 cgcgtcggat gcgacgttgt ggggcaccgc gccgcgcgtg ggaatgtcga actgcgtgat
     2221 gacgatgtcg gtcgcgccgc gcgtgctgcg gtacgcgacc ggcttcggcg cctgtacttg
     2281 cggcgccttg tcgggaccgt aatgccgcgc gagatagcgc gacagccgct cgatctcctc
     2341 gccctttgcc gccatcgcct gctcgtcggg gatctcgcgc cagcgcgcct tgcgcgtctc
     2401 ctcgatccgt cccatgtcga ggatcacctt cttccagccg agctggtcga agcgccggga
     2461 gacgaaattg accgggccgt ggcagttgac gcagcggttc acgacgagct gcttggcgcc
     2521 gtcgccgtcg tcggggagct gcgggatcca gtcggccgtc gtgacgaccg ggtccgaggc
     2581 gcgcagcgcc aggtcgagct tcgcggcctt cccggtcgcg atctcgatcg tgcgctcggc
     2641 gccaggcgcg tggccgtcgg ggccggcgct gacctcggtc ttcccgggga agagcccggc
     2701 cgcgcgatag cggccggcgc gcgagaacac cgtgacggtc acgccgcgat cgaggttgcg
     2761 gatcttcacc gcggcgcccg tcagcggctt tccggccgcg tcgcggatcg tgccggcaag
     2821 ctcaccgcgg tccgcgccgg cgccggcggc gcctgccgag acgagcgcaa agcacagcag
     2881 cagagctctc atgacgtctc cctccgcggg aaacattagt cgaactggcg gccgtcgatc
     2941 aagagccggt tcggcgcgcc gaagcgccgt agtagaatcc gccttccccg aagaaggaac
     3001 ccacatggcc ctttccgaag ccgaccgcaa gaaggcggtc gagatcctgc tgaacgcgga
     3061 aaaagagaag aagcaggcca cgcagctctc cgtaaccttc cccggcatca ccatcgagga
     3121 cgcgtacgcg atctcgaccg cggtggcgca gcacaagatc aagggcggcg cgaagctgat
     3181 cggccacaag gtgggcctga cgtccaaggc gatgcaggcc tcctcgcaga tcaacgagcc
     3241 cgactacggg cacctgctcg actacatgat gatccccgac ggcgcgaagg tgccgcacgc
     3301 gaactactgc gtgccgcggg tcgaggtcga gctcgcgttc gtcctcggca aggcgctcaa
     3361 aggcccgggc gtcggcctgc tcgacgtgct gcgcgcgacc gagtacgtga tcccggcgat
     3421 ggagatcgtc gacgcgcgca tcgtcaatcc gcgcaagatc ttcgacaccg tcgccgacaa
     3481 cggcgcggcg gctggcatcg tctgcggcgg ccggccgatc aagccgggcg acgtcgacct
     3541 gcgctgggtc ggcggcatca tgtaccgcaa ctccgagatc gaggagaccg ggctcgccgc
     3601 gggcgtgctc ggccaccctg cgctcggcgt cgcctggctc gcgaaccgcc tcggccagaa
     3661 cggcgtgacg ctcgaggccg ggcacctggt gctggcggga tcgttcacgc gcgtggtgtt
     3721 cgcgcagaag ggcgacacgc tgcacggcga cttcggcccg ctcggcggcg tcgcggtgca
     3781 attcgtctag agaggaagcg catcatggcc aaagtcggat tccgcatcct tcccgtcgcg
     3841 aagcgcccgg acaagaagct gctcgccgag ctcgcgaaga tggtgacgcc gcacctctcc
     3901 gacagcatgg agcgcctgta cgcgggcggc gcgcagcttc ggcccatgca cgacggcacg
     3961 aagctctgcg gcccggcctt caccgtgcgc acctcgcccg gcgacaacct gctcgtgcac
     4021 aaggcgatcg acatcgccga gcccggcgac gtcatcgtcg tggacgcggg cggcgtctgc
     4081 gacaacgcca tcatcggcga gctcatgtcg gcgcgcgccg agcagcgcgg cgtcgcgggg
     4141 atggtgatct ggggcgcgat ccgcgattcg aaggagctgc gcgaaggaag cttcccggtg
     4201 tacgcgagcg gcgtcacgca ccgcgggccc tacaagaacg gcccgggcga gatcaacgtc
     4261 gcgatctcga tcggcggcat gaccgtcaat cccggcgatg tcatcgtggg agatgccgat
     4321 ggcctggtcg cgatcccgca ggaccaggcg gaggccgtgc tcgcgtcggc caaggccatc
     4381 ctcgccaagg aagagaaatc gatgaaggag atcaaggccg gcaccgtcga ccgcagctgg
     4441 gtcgacaagg cgctcaagga gaaaggctac caggtgtgac catggaccta ccggtcaaca
     4501 cgttcaagcg cgcgctcaag gccggcaagc cccagatcgg cctgtggtgc agcctgtcgt
     4561 cgcactacgc gatcgagctg gtcgcgggct cgggcttcga ctggctgctg ctcgacaccg
     4621 agcactcgcc gaacgatctc gagtcggtgc tcacgcagct gcaggcggcg gcgccttatc
     4681 ccacgcaccc ggtggtgcgc gtgccgtgga acgacatggt gacgatcaag cgcttcctcg
     4741 acatcggctc gcagacgatc ctgatcccct acgtgcagaa cgcggaagaa gcgaaggccg
     4801 cggtcgcgca cacgcgctac ccgcccgcgg gcgtgcgcgg cgtcgcgggc ggcacgcgcg
     4861 cgacgcgctg gggccgggtc aaggactacg cgaagcgcgc gcacgaggag atctgcgtgc
     4921 tggtgcaggt cgagacgcag accgcgctcg acaacctcga gagaatcgcc gcggtcgacg
     4981 gcgtcgacgg cgtcttcatc ggcccggccg atctgcacgc ctcgctcggc taccccggcg
     5041 agaccaacaa cccgaaggtg gtgccggtca tcgacgatgc cgtgcgccgc atccgcaagg
     5101 cgggcaaggc gcccggtatc ctcacgacgg tggaggccga cgcgcaccgc tggctcgagt
     5161 gcggcgggca gttcgtcgcg gtgggcgtcg acaccgtcgt gctcgcgcgc ggcaccgagg
     5221 cgctcgcggc gaagttcaag acctaggacg tcgtatcccg gccgtcgccc gacgggaacc
     5281 ggttcgagcc gtagcggatc acgaggatcg cgagcgcgag cagcaggatc gcgcccgcga
     5341 tcaggatgat tcccatgtcg ggatgcaggt gcttctcctg cacgtcgccg atcatcatgc
     5401 gcgtgagcgc cgtgatcgcg acgtagatca ggaacctcac cggcatatgg ttcgtcttga
     5461 agtagatgcc gaccatcgcg ccgagctcga ggtagatgaa cagcagcagg atgtcgtcga
     5521 tcgaggcgcc gcccttcgcg gccatgcccc agaacgacga gatcgcggac cacacgatcg
     5581 cgccgccgat cgcgaacagc gcgaggtagt ggaaagcgtc gaccaggaag tggccgaccg
     5641 agcccgcgag cccgtccatg gccttgcgca gtctttcggt tctcttcgag tccatcgcgg
     5701 ctccaggttg tcgcgccggc gctaggatac agtagcgacg atgaagaccg tgaagctcac
     5761 cgcgggcgat cccgtcccgg cgctcgggct gggcacctgg cgctacggcg aggaccgttc
     5821 gcgccgcacc gcagagatcg cgacgctgaa gctcgcgctc gacctcggcg cgacgctgat
     5881 cgacaccgcc gagatgtacg gcgaaggcgg cgccgaggag gtgatcggcg aggcgatcgc
     5941 cggccggcgc gacgaggcct tcctcgtcag caaggtctac ccgcacaacg cgacgcgcaa
     6001 aggcaccgcc gccgcctgcg agcgcagcct gaagcgcctg aagaccgacc gcatcgacct
     6061 ctatctcctg cactggcgcc agggcacgcc gctcggggaa tcggtcgagg cgctcgcggc
     6121 gctcgagcga gccggcaaga tccggcactg gggcgtgagc aacctcgact tgtcggacat
     6181 gcgcgagctg tgggagctcg ccgacggccg cggcgtgcag acgaaccagc tcctctacaa
     6241 cctctcgcgc cgcggcatcg agtgggactt gatgccgtgg ctgcgcgagc ggcgcatccc
     6301 ggtgatggcg tactcgccga tcgagcaggc gcggctcgtg agccatcgcg gcctcgccga
     6361 cttcgcgaag cgccacggcg cgacgcccgc gcaggccgcg ctcgcctggc tgctcgcgaa
     6421 ggacgacgtg atcgtcatcc cgaagacgag ccgcgtcgat cgcttgaagg aaaatctcgg
     6481 cgcgctcgag atccgcttct cgaaagacca gctcgccgag ctggaccgcc tcttcccgca
     6541 gccgaaaggg ccgcagccgc tcgagatgct ctaggacagg ttcgcgctca gttcgcgcgt
     6601 gagctgcgcg gccgggatct ccttgcagcc gctcgcgttc tgtcccgacc acagcgagga
     6661 gaagtcggtc ctgccctgcg cctcggcctt cgcgcgcagc gccatgatgg ccgcggtcgc
     6721 gagcggaaac gcgggcgcga gcgtgctgag cggaccgagc tccttcatca cgcggttgac
     6781 gatgccgcgc gccggccgcc ccgtgagaag gttggtgagc gcggtgtggc gcgccgcctc
     6841 gctcttcagg gccgcgcggt gcagcgcgct ggtggtcgcc tcgggacaga gcaggtacgc
     6901 ggtgccgacc tgcgctcctg cggcgccgag cgcgatcgcc gcggcgacgc ctttcgcgtc
     6961 ggcgatcccg cccgcggcga tgaccggcac cttcaccgcg cgcacgacct gcggaacgag
     7021 cgtgaacgtg ccgagctgcg cggtgaggtc gtccgacagg aaattcccgc ggtggccgcc
     7081 ggcctcgacg ccttgcgcga tcacggcgtc gacgccgcgc gcctcgagcc atcgcgcctc
     7141 gtcgacggtc gtcgccgacc cgagcacctt cgcgccgaag gcgcgcacgc gcgcgagcag
     7201 ctccttcgac ggcagcccga aatggaagct caccaccggg ggccggaact cctcgagcac
     7261 gtcggcggcc tcggcgctga acggcagccg cccgggaccc gaggggatcg tcgcggggtc
     7321 gatcccgtac tcggcgaagt agggcgcgag cgctttgcgc caggcggcct cgcgctcggc
     7381 gctcggcgcc ggctgcgcgt ggcagaagaa attgacgttg tacggcttgg tcgttccggc
     7441 gcgcaatgcg gacagctcct tgcgcatcgc ttcgggcgcg agcatggcgc acggcagcga
     7501 ccccaggccg cccgcgttcg aaaccgcgac cgcgagcgcg ctcagctgca cgccggccat
     7561 gggcgcctga acgatcggga ggtcgattcc aagaagagac tgcagtgtcg ccatgttttg
     7621 taccgccgat tcgcgcgccg cgccggatcc ctagatcgta agacgataga gatgtccgcg
     7681 caaatacatg aagtgcgcgc atcgccggca tgcggaccga tcatgcagtt ccacaccgag
     7741 cccgcgcttg cgcggaacca tcaccggcgc gggcgtgccg gtcaagttcg ctctcgacac
     7801 gcggcacctg ctcgccgggg cgtcgtacct cttctaggcg cgcgatctgc tgggcggcag
     7861 cttcatctgc cgacgcggcg ggccttgaga aggaagaaca agaacagcgc acccagaaca
     7921 acgtggatga cgatgccggg cgcgaccgcg gcgccggcgc cgagcgtcat cgtgcgaacg
     7981 acgacggcaa gagacaggat ctgcaccaca agattgccgg caagcaccgc aatgagacct
     8041 tggcccgttg cgctacgtgc cagccaaagg acagtgccca tgcccaccag ggttgcgcca
     8101 tgagcctggg caagcgtggc cagcatgggc gtgagaacca tgccggtggg ttcgtagaac
     8161 ttcgccggca cgaacagcag catggcgccg aagagcccgg ccagagcgcc ggcgatggac
     8221 aggaacagtt tcatgctcgc cccctttcta tctcattcgt aatttgatga cgcctgacgt
     8281 tcgcgttgag cggtcaccga aggtccgctc tcgaacgcgt ggtcagggcg caatgacgac
     8341 atttccgagc ggtacagccg tttcatctga gccaacatat tgaacggagt atgacccagg
     8401 cgcgatgaga ccgagattgg cgggtgggca cttcgcattt cgcccaggtc ccccgatgcc
     8461 tgttatcact cggatgctga tggtctttcc attcacggac gcctgcgtct ccacgcacgc
     8521 cacgcccgaa ttcatggtgg tcggtcttgc ggttatggct ctcaaaccgg aaacgtcgcc
     8581 ttgtaccggc aaaacccagc cccttccgac tttctcgggc cggccaacgg aaaagccacc
     8641 cactgacacc aagtacttcc agctcgccac ctccgcaatg gccgccggag cgaccagtgc
     8701 ccctgctaac acgacgagtg cggcgactgc ttcactccat gctttagcgg gtgggtgttc
     8761 gctcattttg tatgacacct aacgttcaat gatctgcaat ccgcgcatcg tagaaccatg
     8821 tataggcata tgcaaacatc cacccgtata tcgaagcata tgccgttata tggaatcccc
     8881 gcgtagggat ttctcggaca cgacgcgatg ccggatccct ggcgcgcgca cggcttgcca
     8941 agtgatctcc ccggtctttt gcgcgggacg ccagtcgacg cggacgcgca cgtggtcgtg
     9001 gccgtcgcgc cgcgcgagat cgtgtaagct tccccgctcg atgaacgtcc ccgccccgca
     9061 gctctccgag caggtcgacg cgatcttctc cgcctggggc atgtccgagc ccgcgcgcga
     9121 ggcgtgcgtg cggctgatga tcgccgcgga cctgcgcggg atcgactcgc acggcgtgat
     9181 gatgctgccg ctctacgacg agctgcggca ggacgggcgg ctgaagatgg agccgaacgt
     9241 gaggacggtg cgcgagtcgc cggtgacggc gctggtcgac ggcgacatga gcctcggcca
     9301 ttaccccgcg gactacgcga tgaagctcgc gatccgcaag gcgcgcggct cggggctcgc
     9361 ggcggtcgcg gtgcgcaact ccaatcactt cggtgccgcc ggcgtctacg cgatgcaggc
     9421 ggcctccgcg ggcctgatcg ggatcgcgat gacgaacgtg tggaacctcg cgatcgtgcc
     9481 gacgcgcgcg gcgcaggcga tgttcggcac gaacccgatc gcgttcgccg cgcccgcggg
     9541 cgagcagccg ccgttctgcc tcgacatggc gacctccacc gtcgcgatcg gcaagatcaa
     9601 gctcgcgatc ctgcacggca agccgctgcc gcacggctgg gcgcacgcgg ccgacggccg
     9661 catcaccgcg gacgcgaacg cggcgctcgc tgcgcgcctg ctcacgccgg tcggcggcac
     9721 gcccgagctc tcgagccaca agggctacgg cctcgccgcg atggtggaga tcctgtgcgc
     9781 gatgctgccg ggcgcctggt acgcgccgac gcgcgaccag cgccaccccg gcgagaagcg
     9841 ctacaacgtc ggccacttct tcctcgcgat ggacccgaac gcgttccgcg ccgagggcga
     9901 gttcgaggcc gacctcgacg acatgatcgg cgcgctgcgc tcggcgaagc gcctcgaccc
     9961 ggccgagccc gtgctcgtgc acggcgaccc cgagcgcgcg gaaatggagc ggcgcgggcg
    10021 cgagggcgtg ccggtgcccg acgcgctcgc gacgaagatc cgctcgatcg cggccgactg
    10081 cggcgcgccc gcgttgatct gatttcatcg ccggtctgcc gcttttgcac gatctttcgt
    10141 gcaggatccg cggctatcgt gcgaatgaac cattcgggcc agtccggctc ggaggtaatg
    10201 gaaccagtcc agaaggagaa cgaccatgag actcgccccc cgccccgcca ttctcgccgc
    10261 cctcgcctgc acgctcgcgc tttccctgag cgcctgcggc gagcacagca cgcccgccac
    10321 caaatcgacc gacgtcacgc cgccgcagcc ggcgcagcag accgtgcccg ccgcacccgc
    10381 cccggccccg gccgcgcagg ccccgaaggc cgaccccgac gccgagctcg ccgcccgggt
    10441 gaagtccgcg ttcgcgagcg acccgcagct caagatgctg ccgatcgacg tgcgcgcctc
    10501 gaacggcacg gtgacgctct acggcaccgc cgacagcgcc aggctgcgcg acaaggcgat
    10561 ccacacggcc gaggccgtcg acggcgtgaa gtcggtgagc gcgaacctgc agatcgtccg
    10621 cggctcgtga cgcggcgcta gtcgatcttc gcgcccgagc gcttcacgac ctcgccccat
    10681 ttcgccgact cgcggcggat cagctcgccg aactcctcgg gcgtgccgcc gccgggctcg
    10741 ttgccgatgt cgtcgaagcg cttcttcgac gcgggcgtga ggatcgcccg gttgatcgcc
    10801 gcgttcaggc gcagcaccac cggccgcggc agccccgccg ggccgatgac gccggtccac
    10861 gttcccgcct cgtagccggg cacgccggcc tcggcgatcg tcggcaggtc cgggaagccg
    10921 gcggaccgat gcgcgccgct cacggcgagc gcgcgcacgc gtcccgagcg cgcgtagggc
    10981 gcgatcgagt tcaggctctc gaacatcacc tggacgtggc ccgcgatcag gtcgttgatc
    11041 gccatcgagc cgcccttgta cggcacgtgc acgatgtcgg tcccggtcat ggtcttgaac
    11101 agctcgccgc cgacgtggcc cggcgagccg ttgctcgagg acgcgttcag cagcttcccg
    11161 gggttctcct tcgcgtaggc gatcagctcg cgcactgaat gcaccggcag cgagggcgac
    11221 accgcgagca gcaggtggct gcgcacgacg agcgcgatcg gctgcaggtc gcgctcgatg
    11281 tcgtaaggca gcttcgacac catgtggcgc gtgatcgcca tggcgccgat cggccccatg
    11341 ccgacggtgt agccgtcggg cgccgccttc gcgacgagat tgatgccgat cgtgagcgcg
    11401 ccgccggggc ggttctcgac caccacctgc tggccgagct gcgcgggcag ctccgcgacg
    11461 agcagccgtg cgatcgtgtc ggtcgcgctg cccgccgcct gcggcacgat caggcgcacg
    11521 ggatgctcgg gatactcggc gcgcgcgacc gagagccagg cgagcgtaaa gacgacaagc
    11581 ggcacgagca gtctcacggg gttctcctcg aggggcgtcg cgccgtattc tataatcgac
    11641 cgccatgccg aagcagatca tcacctcgcc cgacgcgccg gtcaccgggt tcactgacaa
    11701 gggaacgccc tcaccgctcg cgcaggcgat ccgcgccggc aacctgctgt tcgtgtcggg
    11761 acagggcccg ctcgagccga agacgcgcga ggtcgtcgcc ggcgacatcc gcgcccagac
    11821 gcgtcagacg ctgaccaacc tgaaggcagt cctcgccgcg gccggcctcg acttccggca
    11881 cgtggtgaac atgcgggtcg tgctgcgcaa ccccgcggac tttcccgcgt tcaacgagac
    11941 cttccgcgac ttcctccagg gcgagaaggt cacgcgcacc tgcatcggcg cgacgccgca
    12001 ccgcaaggga gtcgacgtcg agatcgactg cgtcgctagc tacgactgat cggggatgtc
    12061 ctagcgatcc gggcgcgcgt tcattcagtg ccgcccggaa cggtcgccac gctcggggcg
    12121 atcggcgcgc tcgaccttct ccgcacgctc gggcttgtcg gcgcgctcgg gcttgtcggg
    12181 acgatcgggc ttctcggcgc gctcgggtcg cggcgagcgc tcgcgcagct ccacgtgcgt
    12241 gatcgcgtct tcgcgcgcgt ccgggcgtgg gccggcgcgg tcgcggtcgc tgttgtcgac
    12301 gtcgcggaat tcgacgcgca cggcatggcg gccttcgtcg ccgatgcgca ccggctccag
    12361 gccgaaccgc gccgccagct cgcgcagctt attcgcctcg ccggcgctca gcgcggtgtc
    12421 gcggaagcgg atgtcgcgct cgccgcgggt gagcgcggcg cggatctcga cgatcagcct
    12481 cggcacgtcg agcgcgcctg ccgcattccg aggcagcgtc gagcggacct cgcgcgagtc
    12541 gttgcgatcc cggcgagcgc caaggcgatc cacgtcatcg cggaccgcgg ccgtggcagt
    12601 ggcgacgccc cagagcgcga gcgcggcgaa cgcgacggcc ggagcgctga tcttgcgcat
    12661 ggttcgtcga agctagcatg caggcaccgc gcgcagtcac cgcgcgcccg agtccgcatg
    12721 ggctgtcgcc gccttgcgac ggcggcacaa aagacgacgc cgcgcggacg cccggagcgg
    12781 gccgcgcggc gtcggagacg cgccgtcagt gacggcccga ccggtcggga cggtccacgc
    12841 gctcgacctg ttctacccgc tcgacgcgct cgggccgctc tgcgcgctcg gccctttccg
    12901 gacgctcggc gcgctgaggc cggtcatcgg cggcgcggcg gtcgcctcgg gcgtcggcca
    12961 cgcggtcgtc gcgccggccg cggttgtcgc tgctcgtccc gcggtcgtcg ttgcgcacgc
    13021 cgcgagcggc gcggaactcg acgcgcacgc gcttgccggc ggagtccacg ccccggatct
    13081 cgacgcgcgt gaatccgagc gcggcgagct gctgcgcgag ctgcgcccgc tgctcgggcg
    13141 tgaggccggc gagattgacg tcttcgatgc gcgcgcgcag cgtgccgtcg ggctgccgcg
    13201 tcacgcgcac gtccaccgcg ccgcgcaggg tcaccttgcg ctcgagcccg tccttgggca
    13261 gcagcgcggc gatctcggcg agcaggttgt cgctggccgc ggtcgacagg agcaggccct
    13321 gcacgtctgc cgcggtcagg gtgacgccgc ggaactggat ctcgcgcgcg cccgccgcga
    13381 tctggtcacg caccagcgcg agaagcttgt cgacatcgac cgcgcccgtc gccgttctcg
    13441 gcaccgcgag cttcacctcg gcgcgctcgt tctggttttc gttctcgact tcgctctcgg
    13501 tcgcgtcgtt gcgccggtcc atccggcggt cgtcctgacg atcgacgcgc cggtcttcct
    13561 ggcggacgtc gatcgacgac tgcgcctgcg cgaacccggc gggcgcgagg accgcgaggg
    13621 ccgccgccgt cacgaacaga tgcttggtcc acatgatcga atcctttcgg cttggaggtt
    13681 gggggctgct acgacggtcc cggcccgccg gggagggagg cgggccgggc cggcgatgcc
    13741 ggcacgatgc tcagcgccgg cccgagcgat cgggactttc gacccgttcg gctcgctcgg
    13801 ttctttccgc gcgctccggg cgctccgccc gctcggcgcg ttcgatccgt tccgggcgat
    13861 ccatcgcgtc gtcgcgacgg tccatccgcc ggtcctgctg gcgatccgcc ctgcggtcct
    13921 gctgccggtc ggcttggcga tcctgctgcc ggtcttcctg ccgcacgtcg ttcgcgtcgg
    13981 cggtctgcgc cgggacctct tgcgccccga gcagcccgag cgatgcgatc gctactgctg
    14041 ccatcaacgg tttctgccac a
//
LOCUS       DATDMW010000167         6799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_795874, whole genome shotgun sequence.
ACCESSION   DATDMW010000167 DATDMW010000000
VERSION     DATDMW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795874"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VI319_07230"
     CDS             complement(<1..321)
                     /locus_tag="VI319_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6863679.1"
                     /translation="MKRSACLLAMVLGASGAVAQQYPAKPVKIIVAFSPGGSTDLLAR
                     AVGQKLGERWGVPVVVENRVGASGMIGADAVAKSAPDGYTLLLTSMGGQAAGPALYKK
                     LNYDP"
     gene            complement(352..1059)
                     /locus_tag="VI319_07235"
     CDS             complement(352..1059)
                     /locus_tag="VI319_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY6863680.1"
                     /translation="MCGRYVSPEEAAIARAFRLAARANPLARRFNVCPTDTVPFVRAR
                     LDTGEPEIASGRWGLVPHWWKEAKAPRASFNARVEEAASKPMWRDALARSRCLVPAEG
                     WYEWGAAERTDPATGEIVAAKQPRYIRRADGALVCFAGLAAYWKDPKTGEALRSCAIL
                     TAAASGALAAIHGRAPVVLPESAWSAWLDRGLDDPGAARALAARAMPPEAFVHWPVRL
                     LVNDAKRDGPELIEPER"
     gene            complement(1079..2041)
                     /locus_tag="VI319_07240"
     CDS             complement(1079..2041)
                     /locus_tag="VI319_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863681.1"
                     /translation="MRTMFRWLAALALAGVIPATHAQQYPAHPVRMIVPFPAGGPADL
                     VARLLSANLARVWGQPLVIENHGGAGGNIGTAQVAKAAPDGYTLLLTTSGLMSNPSLY
                     KDPGFDPVKDFAPISVVGNSATVVVRHPSLAGVNTMKDVERLARTTKVDFATPGIGLA
                     GHLSGELFRTMTHVEMQAIPFAGAAPAIASVLSGQVKLGFMAVPTTVPHIKSGKLVAI
                     AVTSPKRLAVLPDVPTVAESGYPGFQVDNMYGVLAPAGTPQTIVRKVHADILRVLAMP
                     EVKQALDRATIDIVGNTPEEFADYIRSEYTKWAEVVKASGAKVE"
     gene            2110..2532
                     /locus_tag="VI319_07245"
     CDS             2110..2532
                     /locus_tag="VI319_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6863682.1"
                     /translation="MPGPKIATKHRGSCLCGAIRYEIDGEIGPIQYCHCRRCRKATGS
                     AFNVGSTVPASGFRIVAGDDHLGRFLHTSGLERTFCRACGSPIYSWWPKKAPELRRIR
                     VGTLDTPVPGKVSAHIFTASRAEWDEILDGAPQYAERP"
     gene            2618..3067
                     /locus_tag="VI319_07250"
     CDS             2618..3067
                     /locus_tag="VI319_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863683.1"
                     /translation="MKNMRRALVAALVSLPLAAAAEVRVPEKIAYDKDAAVTDAVKKE
                     CALDTKIPQYVKKAAGSAGSGTLDMKITNVLAPGGGAWSGPKAVTVSGTLREGGKVVG
                     SFTVRRQTTRGGYGTCQMLQRDAEAIGKDIGAWVKSPSMDAKLGDAK"
     gene            complement(3071..4036)
                     /locus_tag="VI319_07255"
     CDS             complement(3071..4036)
                     /locus_tag="VI319_07255"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863684.1"
                     /translation="MKTTIAVALLALASLYGEAASAQDYPTRPIHLIVAFPPGGSVDT
                     VARVIGPPLGTSLGQPVVVENRSGASGNIGTEAAARARPDGYTLLIHTIPFVANASLY
                     PRMPYDPLADFAPVSLLCASPAVLIAHPSLPAKSIAELLALARAKPGALNYSAAGAGT
                     NPHMAGELFNMLGGVDIVAVQYKGGGPALVAVLAGEVPITFQNISEAVPHVHSGKLRA
                     LGVTSAKRAAALPDVPTIAEAGVPGYEFTTWHGVLAPRGTPGDVVKLLNARLAQVLHS
                     GDVSARFAQMGLDVIASTPEEFSAHLKKELDKWGKLVRERHIKVE"
     gene            4141..4767
                     /locus_tag="VI319_07260"
     CDS             4141..4767
                     /locus_tag="VI319_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY6863685.1"
                     /translation="MERSIAVCLLLSLFALAGCAHAPSGGGWITLLDGRTLENWNRIG
                     DANWRIEDGAAVADAGNGFLVTKQTFGDFELRAEFYVDENANSGVFIRCSDPKKPASA
                     TGYEVNIFDKRPDPSYGTGAIVNVAKAQPALKAGGQWNTYEITARGDQLTVVLNGVRT
                     AFARDGKHPSGYIALQHGEDSVKNTALVKFRKVEIRSLAPSGPAKPAY"
     gene            complement(4761..5120)
                     /locus_tag="VI319_07265"
     CDS             complement(4761..5120)
                     /locus_tag="VI319_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6863686.1"
                     /translation="MNGRIDVMLSAAKHLLLVFAVALPFSAHAASAEKGKQAFISHGC
                     WQCHGFQGQGGSAGKQLAPNVMPLAAMSGFVRNSNGPMPPYTKAVLSEDELADIHAYL
                     ESVPKPRDYKSIPQLAQ"
     gene            complement(5117..>6799)
                     /locus_tag="VI319_07270"
     CDS             complement(5117..>6799)
                     /locus_tag="VI319_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6863687.1"
                     /translation="CASSYRGIHESLIDYGGNKMPEFLTCMHEESAVAMGHGYFKIAG
                     KPLMTLCHGTVGLQHASMAIYNAWVDRVPVIVCGGNDLDAAHRPPGVPTTHSAQDINA
                     LVRDFTKWDDQPVSLQHFAQSFVRAYKIATTPPYGPVMLSLDAGLQQEPIRDHGGEKL
                     YIPKFVPTAPPQGDSGAVREAAKMLVGAQNPVIVADRCARTAAAIPMLVELAELLGAR
                     VVDQGSRVNFPRTHPLQSPPTAVANADVILGLELNDFWGTVNAFTDNGEGGIGVNRTR
                     IKPGTKLISISSVELNTKSNYQDFQRFQSVDVSMAGDAEATLPALIEAVKRAGPNKGA
                     AESRIAAARKAHAEAHERARAAAAVAWDASPVSTARLVMETYAAIKNLDWSLVIAEGS
                     GFTWPFRLWPIDKHYQWIGRSGGAGVGYAAPASVGAALANRDAGRFTVGFQSDGDLMY
                     APGVLWTAARHKIPLLSVMHNNRAWHQEVMHVQRMANFRNRKVNTGADLGPVGTSIMN
                     PDIEYHKLAESMGWWAKGPIKDPAQLGPALREAVKVVQSGQPALLNVWTQPR"
BASE COUNT          973 a         2390 c         2422 g         1014 t
ORIGIN      
        1 cgggtcgtag ttgagcttct tgtagagcgc cggcccggcc gcctggccgc ccatcgaggt
       61 gaggagcagc gtgtagccgt cgggcgcgct cttcgccacc gcgtcggcgc cgatcatgcc
      121 gctcgcgccg acgcggttct cgaccacgac cggaacgccc cagcgctcgc cgagcttctg
      181 cccgaccgcg cgcgcgagca ggtccgtgct gcccccgggc gagaacgcca cgatgatctt
      241 cacgggcttc gcggggtact gttgcgcgac ggcgccggag gcgccgagga ccatcgcgag
      301 caggcaggcg ctgcgcttca tcgatcgctc ctgggagtgg ggccgtgcat tctaccgctc
      361 gggctcgatc agctccggcc cgtcgcgctt cgcgtcgttg accaggagcc gcacgggcca
      421 gtgcacgaag gcctcgggcg gcatggcgcg cgcggcgagc gcgcgcgccg ccccgggatc
      481 gtcgagccca cggtcgagcc aggcgctcca ggcgctctcg ggcaggacga cgggcgcgcg
      541 gccgtggatc gccgcgagcg cgcccgacgc ggccgcggtg aggatcgcgc acgagcgcag
      601 ggcctcgccg gtcttcgggt ccttccagta tgcggcgagc cccgcgaagc agacgagcgc
      661 gccgtcggcg cggcggatgt agcgcggctg cttggccgcc acgatctcgc cggtcgcggg
      721 atcggtgcgc tcggccgcgc cccattcgta ccagccctcc gccgggacca ggcagcgcga
      781 gcgcgccagc gcgtcgcgcc acatcggctt cgacgccgcc tcctcgacgc gcgcgttgaa
      841 gctcgcgcgc ggcgccttcg cttccttcca ccagtgcggg accaggcccc agcggcccga
      901 ggcgatctcg ggctccccgg tatcgagccg ggcgcgaacg aaagggacgg tatcggtggg
      961 acagacgttg aagcggcgcg cgagcggatt ggcgcgcgcc gcgagacgga aggcgcgcgc
     1021 gatcgcggct tcctcgggac tgacgtagcg gccgcacatg gccgctattt taggcgccct
     1081 actcgacttt cgcgcccgag gccttcacca cctcggccca cttcgtgtac tcggagcgga
     1141 tgtagtcggc gaactcctcg ggcgtgttgc cgacgatgtc gatcgtcgcg cggtcgagcg
     1201 cctgcttcac ctcgggcatc gcgagcacgc gcaggatgtc ggcgtgcacc ttgcgcacga
     1261 tcgtctgcgg cgtgccggcg ggcgcgagca cgccgtacat gttgtcgacc tggaagccgg
     1321 gatagcccga ctcggccacc gtcggcacgt cgggcagcac cgcgagccgc ttcgggctgg
     1381 tcacggcgat cgcgaccagc ttgccgctct tgatgtgcgg caccgtcgtg ggcaccgcca
     1441 tgaagccgag cttcacctgc ccggagagca ccgacgcgat cgcgggcgcg gcgcccgcga
     1501 acgggatcgc ctgcatctcg acatgcgtca tggtccggaa cagctcgccc gagaggtgtc
     1561 ccgcgagccc gatgccgggc gtcgcgaagt cgaccttcgt ggtgcgcgcg agccgctcga
     1621 cgtccttcat ggtgttcacg cccgcgaggc tcggatggcg cacgaccacg gtcgccgagt
     1681 tgccgacgac gctgatcggc gcgaagtcct tcaccgggtc gaagcccggg tccttgtaga
     1741 ggctcgggtt cgacatcagg cccgacgtgg taagcagcag cgtatagccg tccggcgccg
     1801 ccttcgcgac ctgcgcggtg ccgatgttgc cgccggcgcc gccgtggttc tcgatcacga
     1861 gcggctggcc ccagaccctg gcgagattcg ccgacagcag ccgcgcgacc aggtcggcgg
     1921 gcccgcccgc ggggaacggg acgatcatgc gcacggggtg cgcgggatat tgttgcgcgt
     1981 gggtcgcagg aatcacgccg gcgagcgcga gcgccgccag ccatcggaac atcgttcgca
     2041 ttggggcctc ctcgcccgca ttctaaggtc ggacgataat ggcggcacgc cattccgtgg
     2101 agaggaacga tgccgggacc caagatcgca acgaagcatc gcggcagctg cctgtgcggc
     2161 gcgatccgct acgagatcga cggcgagatc gggccgatcc agtactgcca ctgccgccgc
     2221 tgccgcaagg ccaccgggag cgcgttcaac gtcggctcga ccgtgccggc ctcgggcttc
     2281 cgcatcgtgg cgggcgacga ccacctcggg cgcttcctgc acacgtcggg gctcgagcgc
     2341 acgttctgcc gcgcctgcgg ctcgccgatc tacagctggt ggccgaagaa ggcgcccgag
     2401 ctgcggcgca tccgcgtcgg cacgctcgac acgcccgtgc cgggcaaggt ctccgcgcac
     2461 atcttcacgg cgtccagggc cgagtgggac gagatcctgg acggcgcgcc gcagtacgcc
     2521 gagcgtccgt gacgaaatgt gctttcgcgc ggcggcggcc tttgctacgc ttcccgtcac
     2581 ggtgctcgtc gccggacaac cccagaaggg agagaccatg aagaacatgc gccgggcgct
     2641 cgtcgccgcg ctcgtctcgc tgccgctcgc cgcggcggcc gaggtcaggg tgcccgagaa
     2701 gatcgcctac gacaaggacg ccgcggtcac cgacgcggtg aagaaggagt gcgcgctcga
     2761 caccaagatc ccgcagtacg tgaagaaggc cgccggatcg gccggcagcg gcacgctcga
     2821 catgaagatc accaacgtgc tcgcccccgg cggcggcgcg tggagcggcc cgaaggcggt
     2881 cacggtctcg ggaacgctgc gcgagggcgg caaggtcgtc ggcagcttca ccgtgcgccg
     2941 ccagaccacg cgcggcggct acggcacctg ccagatgctc cagcgcgacg ccgaggcgat
     3001 cggcaaggac atcggcgcct gggtcaagtc gccttcgatg gacgcgaagc tgggcgacgc
     3061 gaagtaatcg tcactcgacc ttgatgtgcc gctcgcggac gagcttgccc catttgtcga
     3121 gctccttctt gaggtgcgcc gagaattcct cgggcgtgct ggcgatgacg tcgagtccca
     3181 tctgcgcgaa gcgcgccgag acgtcgcccg agtgcagcac ctgcgcgagc cgcgcgttga
     3241 ggagcttcac gacgtcgccc ggcgtgccgc gcggcgcgag cacgccgtgc caggtcgtga
     3301 actcgtagcc cgggacgccg gcttcggcga tcgtcggcac gtcgggcagc gccgccgcgc
     3361 gcttcgcgct ggtcacgccg agcgcgcgca gcttgcccga gtgcacgtgc ggcacggcct
     3421 ccgagatgtt ctggaaggtg atcggcacct cgcccgcgag caccgcgacc agcgccggcc
     3481 cgccgccctt gtactgcacg gcgacgatgt cgacgccgcc cagcatgttg aagagctcgc
     3541 ccgccatgtg cgggttggtg cccgcgcccg cggccgaata gttgagcgcg cccggcttcg
     3601 ctctcgcgag cgcgagcagc tcggcgatcg acttcgcggg caggctcggg tgcgcgatca
     3661 gcaccgcggg cgaggcgcac aggagcgaca ccggcgcgaa gtcggcgagc gggtcgtacg
     3721 gcatgcgcgg gtacaggctc gcgttggcga cgaacgggat cgtgtggatg agcagcgtgt
     3781 agccgtcggg cctggcgcgc gccgcggctt cggtgccgat gttccccgag gcgccgctgc
     3841 ggttctcgac cacgaccggc tggccgaggc tcgtcccgag cggcgggccg atcacgcgcg
     3901 ccaccgtgtc gacgctgccg cccggcggga acgcgacgat caggtggatc gggcgggtcg
     3961 ggtagtcctg cgccgatgcc gcttctccgt acagggacgc gagcgcgagc agcgcgacgg
     4021 cgatcgtggt cttcacggga acctcctccg cgccagagtg tagcgaaaga accgtcgagc
     4081 cgggtttacc cggccttctt ttttggagcg atactgggcg ccccgctcga aggaggcgcc
     4141 atggaacgct cgatcgcggt ttgcctgctc ctctccctgt tcgcgctcgc cggctgcgcg
     4201 cacgcgccct ccggcggcgg ctggatcacc ctgctcgacg gcaggacgct cgagaactgg
     4261 aaccgcatcg gcgacgcgaa ctggcgcatc gaggacggcg cggcggtggc cgacgccggc
     4321 aacggcttcc tggtgacgaa gcagaccttc ggcgacttcg agctgcgcgc cgagttctac
     4381 gtcgacgaga acgcgaacag cggcgtcttc atccgctgct cggatccgaa gaagccggcg
     4441 agcgcgaccg gctacgaggt gaacatcttc gacaagcgcc ccgacccgag ctacggcacg
     4501 ggcgcgatcg tgaacgtcgc caaggcgcag cccgcgctca aggcgggcgg ccagtggaac
     4561 acctacgaga tcaccgcccg aggcgaccag ctcaccgtgg tcctgaacgg cgtgcgcacc
     4621 gcgttcgcgc gcgacggcaa gcacccgagc ggctacatcg cgctccagca cggcgaggac
     4681 tcggtgaaga acaccgccct cgtcaagttc cgcaaggtcg agatccgctc gctcgcgccg
     4741 agcggaccgg cgaagcccgc ctactgagcc agctgcggga tgctcttgta gtcgcgcggc
     4801 ttcggcaccg actccaggta ggcgtggatg tcggcgagct cgtcctcgga gaggaccgcc
     4861 ttcgtgtagg gcggcatcgg gccgttgctg ttgcgcacga accccgacat cgcggcgagc
     4921 ggcatcacgt tcggcgcgag ctgcttgccc gccgagccgc cctggccctg gaagccgtgg
     4981 cactgccagc agccgtggct gatgaaggct tgcttgcctt tctccgcgga ggcggcgtgt
     5041 gccgagaacg gcaaggcgac ggcgaaaacc aaaagcagat gcttcgctgc gctcagcatg
     5101 acatcgattc tcccgttcat cgcggctgcg tccagacgtt gagcagcgcg ggctggcccg
     5161 actgcacgac cttcaccgcc tcgcgcagcg ccggcccgag ctgcgccggg tccttgatcg
     5221 ggcccttcgc ccaccagccc atcgactcgg cgagcttgtg gtactcgatg tcgggattca
     5281 tgatgctcgt cccgacgggc ccgaggtcgg cgccggtgtt gaccttgcgg ttcctgaagt
     5341 tcgccatgcg ctgcacgtgc atcacctcct ggtgccaggc gcggttgttg tgcatgaccg
     5401 agagcagcgg gatcttgtgc cgcgccgcgg tccagagcac gcccggtgcg tacatcaggt
     5461 cgccgtcgga ctggaagccg acggtgaagc gtccggcgtc gcggttcgcg agcgccgcgc
     5521 ccaccgacgc cggcgccgcg tagccgacgc ccgcgccgcc cgagcggccg atccactggt
     5581 agtgcttgtc gatcggccag aggcggaacg gccaggtgaa gcccgagccc tcggcgatca
     5641 cgagcgacca gtcgaggttc ttgatcgccg cgtaggtctc catcacgagc cgcgcggtgc
     5701 tcaccgggct cgcgtcccag gcgaccgcgg ccgctgcccg cgcgcgctcg tgcgcctccg
     5761 cgtgcgcctt gcgcgcggcc gcgatgcggc tctcggccgc gcccttgttc gggccggcgc
     5821 gcttcaccgc ctcgatcagc gcgggcagcg tcgcctcggc gtcgcccgcc atggagacgt
     5881 cgaccgactg gaagcgctgg aagtcctggt agttcgattt cgtgttcagc tcgaccgagc
     5941 tgatcgagat gagcttcgtg cccggcttga tgcgcgtgcg gttcacgccg atgccgccct
     6001 cgccgttgtc ggtgaacgcg ttcaccgtgc cccagaagtc gttcagctcg aggccgagga
     6061 tgacgtcggc gttcgcgacc gcggtcggcg ggctctgcag cgggtgcgtc ctcgggaagt
     6121 tgacgcggct gccctggtcg acgacgcgcg cgccgagcag ctcggcgagc tcgaccagca
     6181 tcgggatcgc ggccgcggtg cgcgcgcagc ggtcggcgac gatcaccggg ttctgcgcgc
     6241 cgaccagcat cttcgccgcc tcgcgcaccg cgcccgagtc gccctgcggc ggcgcggtcg
     6301 gcacgaactt cgggatgtag agcttctcgc cgccgtggtc gcggatcggc tcctgctgca
     6361 gccccgcgtc gagcgagagc atcaccgggc cgtacggcgg cgtggtcgcg atcttgtacg
     6421 cgcgcacgaa ggactgcgcg aaatgctgca gcgacaccgg ctggtcgtcc cacttggtga
     6481 agtcgcgcac cagcgcgttg atgtcctgcg ccgagtgcgt ggtcggcacg cccggcggcc
     6541 ggtgcgcggc gtcgaggtcg ttgccgccgc agacgatcac cggcacgcgg tcgacccagg
     6601 cgttgtagat cgccatcgac gcgtgctgca ggccgaccgt gccgtggcac agcgtcatca
     6661 gcggcttgcc cgcgatcttg aagtagccgt gccccatcgc gaccgcggat tcctcgtgca
     6721 tgcacgtgag gaactcgggc atcttgttgc cgccgtagtc gatcagcgac tcgtggatgc
     6781 cgcggtagct cgaggcgca
//
LOCUS       DATDMW010000168        19523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1668201, whole genome shotgun sequence.
ACCESSION   DATDMW010000168 DATDMW010000000
VERSION     DATDMW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19523
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668201"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VI319_07275"
     CDS             complement(<1..869)
                     /locus_tag="VI319_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY6863688.1"
                     /translation="MANAGMVALDWQQRINWDRVRKYRLERARARMKAHGLGAMLLMY
                     DENCRYVTSTLTPGWNRLKPGLRYAILCGDAPPVLFEQGDLGTQVTRHSPWLPKENVR
                     WSYAWIKGAAGPASQQQVKKFTNGLLEEMKRNGVAGEKLGVDFIDINMIQAFQDAKIT
                     WTDGMTPMMEARAVKNEDEQEIARIIGAIGDAAHWETMKFLRPGVTENQVTAHIMQFL
                     YAIPGMEDVEDVIVSSGPNTWPNWRNFSDRIVQPGDIVFMDLAALTWNGYKSCYYRTY
                     CVGKEPTQEQKDY"
     gene            complement(941..1909)
                     /locus_tag="VI319_07280"
     CDS             complement(941..1909)
                     /locus_tag="VI319_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863689.1"
                     /translation="MLKPAIALSLALAASCALGQAYPSKPVHMIIPFAPGGASDFVGR
                     IIQPKMGELLGQQIVIENRGGAAGNIGMEAAAKADPDGYTIFLGNIGTIAINAAVFRS
                     LAINPMRDFIAVTEVVDVPGVLIANKAFPPNSVREVVAYAKANPGKLNYGSPGSGSQN
                     RLEMELFRQAAGGLDMVHIPYKGGAGPAVTGLVAGETHLMFTTAASALPYIKGGRLKA
                     LAVTSAKRISALPDTPTMVESGFPRSVTGSWQGIFVPAGTPSPVVDRLFAVTQQVMRT
                     KDVVDRLATGGVDVVLSDSPKAFADFVAAESQRWGRVAREVGATVD"
     gene            complement(1931..3067)
                     /locus_tag="VI319_07285"
     CDS             complement(1931..3067)
                     /locus_tag="VI319_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6863690.1"
                     /translation="MPKKNPKRIGLAIIGGGRVGLFRGEVAARHPQVDFIGLAELKPD
                     RAKEVGPKIKADFVTDDFRALLARPEVNAAIIATDEHLHVEPILAAVERKIPLLIEKP
                     LATTLADSERVLKAIRTAGVDAVIGYTQRFRRRWLAAKEKVRTGALGDVTLVTSRAFM
                     NRLVALDNYKRTDDPSTISPMVISGTHALDIVMWMMEAKKPVEIYARSVDRALGPLCR
                     GIDATAGVITFEDGSLYHSVISWALPIVWPGAVYSLEVGITGTEGVLTIDDTHRDIVL
                     ATTGASGEGYAPDQARRVDFMGSYPPGDVALGELRGPMREETESWLNRVALGVPTQHA
                     TAAEAHDRLMLTKAFDLSARLKKAIPLPIAAEDERGREALRRAG"
     gene            complement(3207..4271)
                     /locus_tag="VI319_07290"
     CDS             complement(3207..4271)
                     /locus_tag="VI319_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6863691.1"
                     /translation="MKQIGVAIVGTGWCGGIRAETCAASPLVKSLHLAEIRPERLAEV
                     ARATGSKTATADYKTLLKNDEIEAVYVSATPETTHYPMTRDFLAAGKHVFLEKPIAMT
                     LDEADELIALARKNKLKFSIGYSQRFNPKFAYVRKSIREGTIGTPVTALVSRHITRGL
                     GKKISGRVKLSPAAMEATHDLDFVLWCLEPAKPIRVYSQTNYGAMQQSSGQPIPDQQW
                     ISVTLDSGLSFIVGGGWSLPPGYPNFSTTWIEMVGTEGAVMVDDSHRDVVLNTMSKGM
                     QLPMSTMPGEFVEHTYAGPMKAETLHFLEAVVHDRPVMVTPEHARMVMEVYIAADLSA
                     EKNEPVSLPMKPAKSVRAAA"
     gene            4390..5586
                     /locus_tag="VI319_07295"
     CDS             4390..5586
                     /locus_tag="VI319_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6863692.1"
                     /translation="MVSDQLRILIVGGGIGGVATALALAKRGLASRVFEKASEFGEIG
                     YGIQQGPNAYRMLDWLGVMKDLEPQAVFTKNLILIDALTDRELTRIDCGPKFRARYGA
                     PYTVVHRRDLHGALLEACRRRPEIELSTSKELVDFEDRGASVVARFADGSEAEGRVLI
                     GADGIRSRVREIVIGDGHPRPAGHVTYRGVVPQDQIRDPSHFDDMVIWIGPNLHFVQY
                     RLRGGTVMNNVATVVSRRFEAGERERYGGPDELMEVFSRTTPHVQEMLTYVGKDKNWV
                     LHDREPNPEWVKGNVCLLGDAAHPTLQYLAQGACMAIEDGVVLSAKIAGALKGGGTLE
                     PALLAYRDERYMRTTRVVVTSRMFGAIIHCDGGSRDLRNWLVEQRDPHNAWEVDWLYK
                     GIPVPA"
     gene            complement(5588..6550)
                     /locus_tag="VI319_07300"
     CDS             complement(5588..6550)
                     /locus_tag="VI319_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863693.1"
                     /translation="MRPDTKTILASVLALAAALLAADAAAQAYPEKPIRFIVGPGPDS
                     LARVFGQKMTEAWGQPVIVEQRGGGGGTISAEAVAKAPPDGYTLLLATGTHTINPSVY
                     KTSYDIVRDFAPVTLIGSTPFVLAVHPSVPVHSVAELIALAKSKPGKLNYGSGGSGTP
                     PHLATEMLKVMAGIDMVHVPYKTVPAAITDLVAGQIQVMFTVGPAGLPQIRAGRIRGL
                     AVSTAKRSAFAPELPTVAESGLPGYDVFGWNGVLVPAGTPKAIIAKLHDAIVGAMKEP
                     AVRERMAGFGFEPVGNTPEEFGEFVKTDIARWAKVVKQSGAKAE"
     gene            complement(6572..6907)
                     /locus_tag="VI319_07305"
     CDS             complement(6572..6907)
                     /locus_tag="VI319_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863694.1"
                     /translation="MNPIRRGLAAVCSVVLVAACAPDTLKSDPAYNSFLDQVQKDCYF
                     ARIGIFNVGDLLKNSGASDSVYFIDETSRLYAGEITRQSWTSAITAFLTGRESDPGVQ
                     CVLKHLPNR"
     gene            complement(6904..7782)
                     /locus_tag="VI319_07310"
     CDS             complement(6904..7782)
                     /locus_tag="VI319_07310"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY6863695.1"
                     /translation="MSHAFDKVAGPYEGGTAGVAWDGSAMLFSAPALGQILRYDPKSG
                     KTSVLRKYTNRTSGIAFAPDGALYGCQENSRRVVRFEADGSTTTTAFLLDGRYHNHPN
                     SLAIDAQGRIWFSDPWSELRASGPQIFPPLEHASVLRLELDAFRKLWAIRRMTYDTAS
                     PRAVALAPDGRTLYVAETDNRPGGTRELRAYPIREDGTLGAFVLLHVFGSDHRGVHRG
                     VEGMCVDREGQVYACAGWKRSGPGPLVYVFSPSGAVVETHSVPADEPVGCAFGGGDLY
                     VTTGRGELYRARDIAT"
     gene            complement(7784..8677)
                     /locus_tag="VI319_07315"
     CDS             complement(7784..8677)
                     /locus_tag="VI319_07315"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY6863696.1"
                     /translation="MLELDRATPALARLIAHDAPLDRLHHGMSFGEGPLWNRRDGSFF
                     FVDIVGDTIWRWRPGTGSEPFIRPSTKANGLTFDREGRLCVAGWASRSVWRVEKDGSV
                     KTLASHYDGKKINSPNDIVVKSDGAIYFTDSPGALYNVGMAAEDVQQYLDFAGVFRIA
                     PDGRVDAVITDTVYPNGLCFNLDESVLYVNDTRLGEIRAYDMKRDGSCGAMRLLHKLT
                     GHEPGIADGMKLDREGNVWCTGPGGIHVIAPDGKLLGRLRIPDHCTNLAWGGEDWRSL
                     YITTYASVYVTRANVAGVAVG"
     gene            complement(8664..9119)
                     /locus_tag="VI319_07320"
     CDS             complement(8664..9119)
                     /locus_tag="VI319_07320"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863697.1"
                     /translation="MIDALAVRRVVTLDDERGRSRAIADGPAPDLRADPFQPGAMCSL
                     IWATDSTPATGAAQPARVGDDLSPPRGGSVCRIVTIPPDAGRSPQMRHARTLDFTLVL
                     EGSITLILDTGEVELNAGDTLVQRATRHAMSNRSGRPCVLAISSHDARA"
     gene            complement(9116..9673)
                     /locus_tag="VI319_07325"
     CDS             complement(9116..9673)
                     /locus_tag="VI319_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863698.1"
                     /translation="MKQGVKPIRRVVTGNDAQGRSRVLFDGPAPNVNPGAVAASAAMT
                     DLWVFESCPVDVSGERDCGNLPFHFEPPESGGHLRIVQSAGKPAEYDAAKDPSAVPVH
                     EPRRRPGGTWDKGGQNAFSSPIHKSDTVDYGIVLEGERVLRLDDGERVMKPGDVVVQL
                     GNWHGWTNPRGPSLMAFVMMGAKLP"
     gene            9738..10607
                     /locus_tag="VI319_07330"
     CDS             9738..10607
                     /locus_tag="VI319_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY6863699.1"
                     /translation="MRLCRFDKNRLGIVQGGEVLDVTKALDVIPEQRWPLAQGDPLIL
                     NLKKVIAAAKKLAPRAKRKPLAKVKLLSPVANPGKIIAAPINYNDHIAESIKDPGIAH
                     GRTNIAKGIGDWGLFLKAGSSLIGFGEEIRLRWPERRNDHEVELAVIIGKKGNRIPRD
                     RAMDYVCAYSIGLDMTVRGPELQCFRKSIDTYSVLGPWLVTADEIADPNALDLSIAVN
                     GELRQNSNTRYLVYDVQRLIEFGSAMYTLHPGDVIMTGTPAGVSPVKPGDRLHAKVQG
                     VGEADIRIAAEYA"
     gene            10616..11110
                     /locus_tag="VI319_07335"
     CDS             10616..11110
                     /locus_tag="VI319_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6863700.1"
                     /translation="MAQDDKLQLTSDLRVALQEFYFRYAECLDGGRLEQWPEFFLDAG
                     AYRLTTRRALRLGPDDDIFSLRTKSALRDRIVAIQQSEDYEPHTQRHFISNFRVQVSG
                     ADELRVQANVQVLRTYPGRETEVLCSGQYADRVASAAGRFNFREKCCVLDSDIAPRDL
                     IYPV"
     gene            11193..11594
                     /locus_tag="VI319_07340"
     CDS             11193..11594
                     /locus_tag="VI319_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY6863701.1"
                     /translation="MSGQKSRPSDPVLDQVYRIIGLQVLVTALISLPLLAIFGARVAA
                     SAFAGGAIGFTTAWVYARRMTAASRTGGRSDDPRILLRNHFAAELLKLGLTVVLLAAV
                     LIFFPEVATLPLLLTFMATLAVYWVALLFVF"
     gene            11614..12429
                     /gene="atpB"
                     /locus_tag="VI319_07345"
     CDS             11614..12429
                     /gene="atpB"
                     /locus_tag="VI319_07345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY6863702.1"
                     /translation="MAESAAPLSANEYIGHHLQNLTVGSGFWTLHVDTLLMSGLVGIV
                     VFGLMYWAARSARNGVPGKLQAFVEIVVGMVDEQVRDTFHAESALVTPLAIIIFVWVF
                     CMNAIDLIPVDFIPGFVEHTFHVEHFRPVPTADPNATFAMSLTVFLLIWVFNIRYKGF
                     VGLLREMLTAPFGPWLAPVNFILRLVEEFAKPVSLALRLFGNMFAGEVLFILIVLLAG
                     VWSGPNVGSASSFLGHVVLSLGWAIFHILIITLQAFIFMMLTIVYLSLASESH"
     gene            12465..12734
                     /gene="atpE"
                     /locus_tag="VI319_07350"
     CDS             12465..12734
                     /gene="atpE"
                     /locus_tag="VI319_07350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY6863703.1"
                     /translation="MEALALFFGQTQIAAGLLIGMGALGTAIGFGILGGRFLEGAARQ
                     PEMIPTLQVKMFIVAGLLDAFTAIATAIAVLLLYANPLIGAVKGG"
     gene            12757..13227
                     /locus_tag="VI319_07355"
     CDS             12757..13227
                     /locus_tag="VI319_07355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY6863704.1"
                     /translation="MNVNATLIGQVIWFALFIWFCMKFVWPYFQRIIAERQRTIAEGL
                     AAAEKGRQSLLTSAQQAEAELRGARERAAEIVAQAEKRAAQLVDEARTAARDEGSREK
                     AAAKAEIDQEVSRARETLREQVAALAVAGAEKILRREVNAQAHAELLAQLKKEI"
     gene            13227..13763
                     /locus_tag="VI319_07360"
     CDS             13227..13763
                     /locus_tag="VI319_07360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY6863705.1"
                     /translation="MAETATIARPYAEAVFGLADKTGALAKWSQLLAAMASSAADPRV
                     RALIGDPKVREDQLYGLIAALAGADIPAEAQNLVRVLMANDRLTLLPEISAQFESLRH
                     EREGVVEAEIRTAFPMANGQLEGLVGDLERRFKRKIDAKVVVDKELIGGVRIAVGDEV
                     IDGSVRGKLADMASALKS"
     gene            13794..15344
                     /gene="atpA"
                     /locus_tag="VI319_07365"
     CDS             13794..15344
                     /gene="atpA"
                     /locus_tag="VI319_07365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY6863706.1"
                     /translation="MQQLNPSEISELIRAKIQNLALSADVRTQGTVVTVTDGICRIHG
                     LSDAMQGEMLEFPGNTFGLALNLERDSVGAVVLGAYEHITEGDTVKCTGRILEVPVGP
                     ELLGRVVNSLGQAIDGKGAIATQRSEPIEKIAPGVIHRKSVSQPVQTGLKAIDAMVPV
                     GRGQRELIIGDRQTGKTAVAVDTIINQKGKDLICVYVAIGQKASTIANVVRKLEEYGA
                     MAYTIVVAASASESAAMQYIAPYAGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQISL
                     LLRRPPGREAYPGDVFYLHSRLLERAARVNEEYVEKYTGGEVKGRTGSLTALPIIETQ
                     AGDVTAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKIMKRKDT
                     GGGVRLALAQYRELAAFAQFASDLDEATRRQLERGRMVTELMKQPQYQPLQVWEMALT
                     LFAVNNGYFDDIDVRKALAAERSMRDYLKTKYAPLVKSMEDKKDLPADAEKALKSAIE
                     DWKKSGSF"
     gene            15344..16207
                     /gene="atpG"
                     /locus_tag="VI319_07370"
     CDS             15344..16207
                     /gene="atpG"
                     /locus_tag="VI319_07370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY6863707.1"
                     /translation="MPGTKEIRMKIRSVQNTRKITKAMEMVAASKMRKAQDRMRHARP
                     YGDKIRNVAAHISHANPEYRHPFLVERDTVKGVGIIVVTTDKGLCGALNTNLLRLALT
                     QYKAWQAQGEKVEVCAIGGKGFGFMQRLGANVVSHVVQLGDRPHMDKLIGAVKVMLDG
                     FVADRFDKLMLFYTKFINTMKQEPVMEQLLPLTGEKIGVPQGAWDYIYEPEARAVLDQ
                     VLTRYIEAIIYQAVSENMASEQSARMVAMKAASDNAATLIDELTLIYNKNRQAAITKE
                     ISEIVGGAAAV"
     gene            16216..17667
                     /gene="atpD"
                     /locus_tag="VI319_07375"
     CDS             16216..17667
                     /gene="atpD"
                     /locus_tag="VI319_07375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY6863708.1"
                     /translation="MAEAQSIGTIVQSIGAVIDIEFPREAMPRVYDALVLEDSGKSFA
                     EKGLTFEVEQQLGDGIVRCIALGSSDGLRRGMKVKSTGAPISVPVGQGTLGRIMDVLG
                     RPIDEAGPIQSKEKRSIHQAAPKFDELSPSVELLETGIKVIDLICPFAKGGKIGLFGG
                     AGVGKTVNMLELINNIATQHSGLSVFAGVGERTREGNDFYHEMIEAEVINLKNITESK
                     VAMVFGQMNEPPGNRLRVGLTGLTMAEYFRDEGRDVLMFIDNIYRFTLAGTEVSALLG
                     RMPSAVGYQPTLAEEMGKLQERITSTKTGSITSIQAVYVPADDLTDPSPATTFGHLDA
                     TVVLSRDIAALGIYPAVDPLDSTSRQVDPNTIGEDHYNTTRAVQATLQRYKELRDIIA
                     ILGMDELSPEDKLAVSRARKIQRFLSQPFTVARNFTGQEGKYVPLKDTIKGFKMIVNG
                     ECDALPEQAFYMVGPIEEAFAKAEEMKKETAKA"
     gene            17667..18095
                     /locus_tag="VI319_07380"
     CDS             17667..18095
                     /locus_tag="VI319_07380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY6863709.1"
                     /translation="MAEAHTIHVDVVSAEESIYSGEATFVALPGEAGELGIYPRHTPL
                     ITRIKPGSVRIQPAGGGEEELIFVQGGILEVQPGVVTVLADTAIRGHDLDEAKATEAL
                     ARAEEARKNAKSQEQVATVEAEVATLAAQLAAIRKLRRKK"
     gene            18233..18736
                     /locus_tag="VI319_07385"
     CDS             18233..18736
                     /locus_tag="VI319_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6863710.1"
                     /translation="MTWRVVPMEEQHIEGYRSALDSVARERKYLAFLEAPPVEAVTVF
                     VLGNLRANRPHFVAVDGGEVVGWCDISSLERPAFAHSGMLGMGVLASHRGRGIGRELL
                     RATLENAHEMGLTRVELTVREPNARARELYEKAGFAVEGLKRNAVRVDGRYENLVCMA
                     ILFHEGR"
     gene            complement(18737..19456)
                     /locus_tag="VI319_07390"
     CDS             complement(18737..19456)
                     /locus_tag="VI319_07390"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEY6863711.1"
                     /translation="MYEDLIGEVEDFHGDLVRNIQTIRVSQDLFDDLSDDPAERAVAV
                     AAEGVARIASAAPLITRPFDYGSVVTYPFVEWNGHATRFSDGLAYGVWYGSLELETTV
                     YETVHHRHRFLADAFPGEDRAIANERRVFEVRCDAILIDLRGRERKEKRLVDRSSYAF
                     TQPLGRYLHDQGQNGLLVRSARDIRPKGGVNAALFRAEALANVRDLCHLTYLMNPTKD
                     SARVERTPGRLWMEIRPSRLA"
BASE COUNT         3150 a         6554 c         6744 g         3075 t
ORIGIN      
        1 tagtagtcct tctgctcctg cgtgggctcc ttgccgacgc agtaggtccg gtagtagcag
       61 gacttgtagc cattccaggt gagcgcggcg aggtccatga agacgatgtc gccgggctgg
      121 acgatgcggt ccgagaagtt gcgccagttg ggccaggtgt tcgggcccga cgacacgatc
      181 acgtcctcga cgtcctccat ccccgggatc gcgtagagga actgcatgat gtgcgcggtc
      241 acctggttct cggtgacgcc ggggcgcagg aacttcatcg tctcccagtg cgccgcgtcg
      301 ccgatggcgc cgatgatgcg cgcgatctcc tgctcgtcct cgttcttgac ggcgcgcgcc
      361 tccatcatcg gcgtcatgcc gtcggtccag gtgatcttcg cgtcctggaa cgcctggatc
      421 atgttgatgt cgatgaagtc cacgccgagc ttctcgccgg cgacgccgtt gcgcttcatc
      481 tcctcgagga gaccattcgt gaacttcttc acctgctgct gcgaggcggg gccggcggcg
      541 cccttgatcc acgcgtacga ccagcggacg ttctccttcg gcagccacgg cgagtggcgc
      601 gtcacctgcg tgccgaggtc gccctgctcg aacagcaccg gcggcgcgtc gccgcacagg
      661 atggcgtagc gcaggccggg cttgaggcgg ttccagccgg gcgtgagcgt gctggtcacg
      721 tagcggcagt tctcgtcgta catgagcagc atcgcgccca gcccgtgcgc cttcatgcgc
      781 gcgcgcgccc gctcgaggcg atacttgcgc acgcggtccc agttgatccg ttgctgccag
      841 tccagtgcca ccatgcctgc gtttgccatt acgctctccg tcgttttgag attggtaact
      901 acattgtcgt cctgagcgaa gcgaaggacc tgcttttcgc ttaatccacc gtcgccccga
      961 cctcgcgcgc gacccggccc cagcgctgcg actcggcggc gacgaagtcc gcgaaggcct
     1021 tcggcgaatc gctcagcacg acatcgacgc cgcccgtcgc gaggcggtcg acgacgtcct
     1081 tggtcctcat cacctgctgc gtcacggcga acagccggtc caccaccggg ctcggcgtgc
     1141 ccgccggcac gaaaatgccc tgccaggagc cggtgaccga gcgcgggaag ccggactcga
     1201 ccatggtcgg cgtgtcgggc agcgccgaaa tgcgcttcgc gctcgtcacc gccagcgcct
     1261 tcagccggcc gcccttgatg taggggagcg ccgaggccgc ggtggtgaac atgagatgcg
     1321 tctcgccggc gacgagcccg gtcaccgccg ggccggcgcc gcccttgtac gggatgtgca
     1381 ccatgtccag gccgcccgcg gcctggcgga agagctccat ctcgaggcgg ttctggctgc
     1441 ccgagccggg cgagccgtaa ttgagcttcc cggggttcgc cttcgcgtac gcgaccacct
     1501 cgcgcacgct gttcggcggg aacgccttgt tggcgatcag cacgccgggg acgtcgacca
     1561 cctcggtcac cgcgatgaag tcgcgcatcg ggttgatcgc gagcgagcgg aacaccgcgg
     1621 cgttgatggc gatggtgccg atgttgccga ggaagatcgt gtagccgtcg gggtccgcct
     1681 tggcggccgc ctccatgccg atgttgcccg cggcgccgcc gcggttctcg atcacgatct
     1741 gctggccgag cagctcgccc atcttcggct ggatgatgcg gccgacgaag tcggacgcgc
     1801 cgccgggggc gaacgggatg atcatgtgca cgggcttcga ggggtaggcc tggcccagcg
     1861 cgcacgatgc cgcgagcgcg agggataggg cgatcgccgg tttcagcatt ctctctcctc
     1921 cgtctttcct tcagcccgct cttctcagcg cttcccgtcc gcgctcgtct tccgcggcga
     1981 tcgggagcgg gatcgccttc ttcagccgcg ccgagagatc gaacgccttc gtgagcatca
     2041 gccggtcgtg cgcctcggcc gcggtcgcgt gctgcgtcgg cacgccgagc gcgacgcggt
     2101 tcagccagct ctcggtctcc tcgcgcatcg ggccgcgcag ctcgccgagg gcgacgtcgc
     2161 cgggcgggta gctgcccatg aagtcgacgc ggcgcgcctg gtcgggcgcg tagccttcgc
     2221 cgctcgcgcc ggtcgtcgcg agcacgatgt cgcggtgcgt gtcgtcgatc gtcagcacgc
     2281 cctcggtgcc ggtgatgccg acctcgaggc tgtagaccgc acccggccac acgatcggca
     2341 gcgcccacga gatcaccgag tggtacaggc tgccgtcctc gaaggtaatg acgccggcgg
     2401 tggcgtcgat ccccctgcac aaggggccga gcgcgcggtc gaccgagcgc gcgtagatct
     2461 cgaccggctt cttcgcctcc atcatccaca tcacgatgtc gagcgcgtgc gtgcccgaga
     2521 tcaccatcgg cgagatcgtc gaggggtcgt cggtgcgctt gtagttgtcg agcgcgacga
     2581 ggcggttcat gaacgcgcgc gaggtgacga gcgtcacgtc gccgagcgcg ccggtcctca
     2641 ccttctcctt cgccgcgagc cagcggcggc ggaagcgctg cgtgtagccg atgacggcgt
     2701 cgacgcccgc cgtccggatc gctttcagga cgcgctcgga gtcggcgagc gtggtcgcga
     2761 gcggcttctc gatcagcagc ggaattttgc gctcgaccgc ggcgaggatg ggctcgacgt
     2821 gcaggtgctc gtcggtggcg atgatggcgg cgttcacctc gggacgcgcg agcagggcgc
     2881 ggaagtcgtc ggtgacgaag tcggccttga tcttcggtcc gacctccttt gcgcgatcgg
     2941 gcttgagctc ggcgaggccg atgaaatcga cctgcgggtg gcgcgcggcc acctcgccgc
     3001 ggaacaatcc gacgcggccg ccgccgatga tggcgaggcc gatgcgcttg gggttcttct
     3061 tgggcatttg gcgattagta gggcatttaa accgagaccc ttgattcatt catttgccgg
     3121 tgcgatcgcg ggtgattcga ggtcgtcccg gcaaatgaat gaatcaaggg tcctttgtta
     3181 acggcaaaag caaagacgaa tgcccgctac gccgccgccc gcacgctctt cgccggcttc
     3241 atcggcaggc tcaccggctc gttcttctcc gccgacagat ccgccgcgat gtacacctcc
     3301 atcaccatgc gcgcgtgctc gggcgtgacc atcaccggcc ggtcgtgcac gaccgcctcg
     3361 aggaagtgga gcgtctcggc cttcatcggg ccggcgtagg tgtgctcgac gaactcgccg
     3421 ggcatggtgg acatgggcag ctgcatgccc ttgctcatgg tgttgaggac gacgtcgcgg
     3481 tggctgtcgt cgaccatcac cgccccctcg gtgccgacca tctcgatcca ggtggtggag
     3541 aagttcgggt agccgggcgg caggctccag ccgccgccga cgatgaacga gagcccgctg
     3601 tccagggtga cgctgatcca ctgctggtcg gggatcggct ggccgctcga ctgctgcatg
     3661 gcgccgtagt tggtctgcga gtagacgcgg atcggcttcg cgggctcgag gcaccacagg
     3721 acgaagtcga ggtcgtgcgt cgcctccatc gcggcgggcg agagcttgac gcgcccgctg
     3781 atcttcttgc ccaggccgcg cgtgatgtgt cgcgagacga gcgcggtgac gggcgtgccg
     3841 atcgtgccct cgcggatcga tttcctcacg tacgcgaact tcgggttgaa gcgctgcgag
     3901 tagccgatcg agaacttgag cttgttcttc ctcgcgagcg cgatcagctc gtcggcctcg
     3961 tcgagcgtca tcgcgatcgg cttctcgagg aagacgtgct tgccggccgc gaggaagtcg
     4021 cgcgtcatcg ggtagtgcgt ggtctcgggc gtggccgaga cgtagaccgc ctcgatctcg
     4081 tcgttcttca ggagcgtctt gtaatccgcg gtcgcggtct tcgagccggt cgccctggcg
     4141 acctcggcga ggcgctccgg gcggatctcg gcgagatgca ggctcttcac cagggggctt
     4201 gccgcgcagg tctccgcgcg gatgccgccg caccagccgg tgccgacgat ggctacgccg
     4261 atctgcttca tggggatctc ctgtccggac cgtctccgag gggaagggca ttttcctatg
     4321 ccttgggcgc cgtgtcgatg ggccgcacgt tgatcgcgca gcacacctgg gcctaagatg
     4381 acggcctcca tggtaagcga tcagctccgg atcctgatcg tcggcggcgg catcggcggc
     4441 gtcgccaccg cgctcgcgct cgcgaagcgc gggctcgcct cgcgcgtgtt cgagaaggcg
     4501 agcgagttcg gcgagatcgg ctacggcatc cagcagggcc cgaacgcgta ccgcatgctc
     4561 gactggctcg gcgtgatgaa ggacctcgag ccgcaggcgg tgttcacgaa gaacctgatc
     4621 ctgatcgacg cgctcaccga ccgcgagctc acgcgcatcg actgcgggcc gaagttccgc
     4681 gcgcgctacg gcgcgccgta tacggtggtg caccggcgcg acctgcacgg ggcgctgctc
     4741 gaggcgtgcc gccggcgccc ggagatcgag ctgagcacgt cgaaggagct ggtcgatttc
     4801 gaggaccggg gcgcgagcgt ggtcgcgcgc ttcgccgacg gcagcgaggc ggaagggcgc
     4861 gtcctgatcg gcgccgacgg catccgctcg cgcgtgcgcg agatcgtgat cggcgacggg
     4921 cacccgcgcc ccgcgggcca cgtcacctac cgcggcgtcg tgccgcagga ccagatccgc
     4981 gatccctcgc acttcgacga catggtgatc tggatcggcc cgaacctgca cttcgtgcag
     5041 tatcgcctcc gcggcggcac ggtgatgaac aacgtcgcca ccgtggtcag ccgccggttc
     5101 gaggcgggcg agcgcgagcg ctacggcggg cccgacgagc tgatggaggt gttctcgcgc
     5161 accacgccgc acgtgcagga gatgctgacc tacgtcggca aggacaagaa ctgggtgctg
     5221 cacgaccgcg agccgaatcc cgagtgggtg aagggcaacg tgtgcctgct cggcgacgcc
     5281 gcgcacccga cgctgcagta cctcgcgcaa ggggcgtgca tggcgatcga ggacggcgtg
     5341 gtgctctcgg cgaagatcgc gggcgccttg aaaggcggcg gcacgctcga gccggcgctg
     5401 ctcgcgtacc gcgacgagcg ctatatgcgc accacgcgcg tggtggtcac gtcgcgcatg
     5461 ttcggcgcca tcatccactg cgacggcggc tcgcgcgacc tgcgcaactg gctggtcgag
     5521 cagcgcgacc cccacaacgc gtgggaggtc gactggctct acaaaggcat cccggtcccg
     5581 gcctgaacta ctccgccttc gccccgctct gcttcaccac cttcgcccag cgcgcgatgt
     5641 cggtcttcac gaactcgccg aactcctcgg gcgtgttccc gaccggctcg aagccgaatc
     5701 ccgccatgcg ctcgcgcacc gcgggctcct tcatcgcgcc gacgatggcg tcgtgcagct
     5761 tcgcgatgat cgccttcggc gtccccgcgg gaacgagcac gccgttccag ccgaagacgt
     5821 cgtagcccgg caatccggac tcggcgacgg tcgggagctc gggcgcgaac gccgagcgct
     5881 tcgccgtcga caccgcgagc ccgcggatgc gcccggcacg gatctgcggc aggcccgcgg
     5941 ggccgaccgt gaacatcacc tggatctgcc cggcgacgag gtcggtgatc gccgcgggca
     6001 cggtcttgta cggcacgtgc accatgtcga tgcccgccat caccttcagc atctcggtcg
     6061 cgaggtgcgg cggcgtgccg ctgccgcccg agccgtagtt gagcttgccg ggcttcgact
     6121 ttgcgagcgc gatcagctcg gcgaccgaat gcacgggcac cgacggatgc accgcgagca
     6181 cgaacggcgt cgagccgatc agcgtcaccg gcgcgaaatc gcgcacgatg tcgtacgagg
     6241 tcttgtacac ggacgggttg atcgtgtgcg tgcccgtggc gagcagcagc gtgtagccgt
     6301 cgggcggcgc cttcgccacc gcctcggccg agatcgtgcc gccgcccccg ccgcgctgct
     6361 cgacgatcac cggctggccc cacgcctcgg tcatcttctg gccgaagacg cgcgcgagcg
     6421 agtcggggcc ggggccgacg atgaagcgga tgggcttctc ggggtaggct tgagcggcgg
     6481 cgtcggccgc gagcagcgct gcggcgagcg cgaggacgct ggcgaggatg gttttcgtgt
     6541 cagggcgcat gtttccttgg ctcatggcag atcaacgatt cggtaagtgc tttagcacgc
     6601 actggacgcc agggtcgctt tcccggccgg tgaggaaggc cgtgatcgcc gacgtccagc
     6661 tttgccgcgt gatctcgccc gcgtaaaggc gcgaggtttc gtcgatgaag tagacgctgt
     6721 cgctcgcccc ggagttcttg agcaggtcgc cgacattgaa gatgccgatg cgggcgaagt
     6781 aacagtcttt ctggacctgg tcgagaaaag agttgtaagc aggatcgctc ttcaaggtgt
     6841 ccggcgcaca cgccgccacg agcaccacgc tgcacactgc agcgagaccg cggcggatcg
     6901 ggttcatgtt gcgatgtccc gcgctcgata gagctcgcca cggcccgtgg tgacgtagag
     6961 atccccgccg ccgaaggcgc agccgaccgg ctcgtccgcc ggaacggaat gcgtctcgac
     7021 gacggcgccc gaaggcgaga agacgtagac gagcggtccc gggccgctgc gcttccagcc
     7081 cgcgcacgcg tagacctgtc cctcgcgatc cacgcacatg ccctcgacgc cgcgatgcac
     7141 gccgcggtga tcgctgccga agacgtgcag cagcacgaac gcgccgagcg tgccgtcctc
     7201 gcggatcgga taagcgcgca gctcgcgcgt gccgcccggc cggttgtcgg tctcggcgac
     7261 gtagagcgtc ctgccgtcgg gcgcgagcgc gaccgcgcgc gggctcgcgg tgtcgtacgt
     7321 catgcgccgg atcgcccaga gcttgcggaa ggcgtcgagc tcgaggcgca gcaccgacgc
     7381 gtgctcgagc ggcgggaaga tctgcgggcc cgaggcgcgc agctcgctcc acggatcgct
     7441 gaaccagatc cggccctgcg cgtcgatcgc gaggctgttc gggtggttgt ggtagcggcc
     7501 gtcgagcagg aacgcggtgg tcgtggtcga gccgtccgcc tcgaagcgca cgacgcggcg
     7561 cgaattctcc tggcagccgt agagcgcgcc gtcgggcgcg aaggcgatgc cgctggtgcg
     7621 gttcgtgtat ttgcgcagga cggatgtctt tcccgacttc gggtcgtagc gcaggatctg
     7681 acccagcgcc ggcgcgctga agagcatggc cgagccgtcc caggcgaccc cggccgtgcc
     7741 gccctcgtaa ggcccggcga ctttatcgaa ggcgtggctc atgtcacccc accgccacgc
     7801 ccgcgacatt cgcgcgcgtg acgtacaccg aggcgtacgt cgtgatgtac agcgagcgcc
     7861 agtcctcgcc gccccacgcg agattggtgc aatggtcggg gatgcgcagc cgcccgagga
     7921 gcttgccgtc gggcgcgatg acgtggatac cgcccggtcc cgtgcaccag acgttgccct
     7981 cgcggtcgag cttcatgccg tcggcgatgc cgggctcgtg ccccgtgagc ttgtggagca
     8041 gtcgcatcgc gccgcagctc ccgtcgcgct tcatgtcgta ggcgcggatc tcgccgaggc
     8101 gcgtgtcgtt cacgtagagc accgactcgt cgaggttgaa gcacaggccg ttcgggtaga
     8161 cggtgtcggt gatcaccgcg tcgacgcggc cgtcgggcgc gatgcggaac acgcccgcga
     8221 agtcgagata ctgctgcacg tcctcggccg ccatgccgac gttgtagagc gcgccgggcg
     8281 agtcggtgaa gtagatcgcg ccgtccgact tcacgacgat gtcgttcggg ctgttgatct
     8341 tcttcccgtc gtagtgggag gcgagcgtct tgacgctgcc gtccttctcc acgcgccaga
     8401 cgctgcgcga ggcccagccc gcgacgcaca gccgtccctc gcggtcgaag gtgaggccgt
     8461 tcgccttggt cgagggacgg atgaagggct cggagccggt gcccggacgc cagcgccaga
     8521 tcgtgtcgcc gacgatgtcg acgaagaaga agctcccgtc gcgccggttc cacagcggcc
     8581 cctcgccgaa gctcatgccg tggtgcagac ggtcgagcgg cgcgtcgtgc gcgatcagcc
     8641 gcgcgagcgc gggcgtggcg cggtcaagct cgagcatcgt gcgaggagat ggcgaggacg
     8701 caggggcggc ccgagcggtt gctcatcgcg tggcgcgtcg cgcgctgcac cagcgtgtcg
     8761 ccggcgttca gctcgacttc cccggtgtcg aggatcagcg tgatgctgcc ctcgagcacg
     8821 agggtgaagt cgagcgtgcg ggcgtgccgc atctgcggag agcggccggc gtcgggcggg
     8881 atcgtcacga tgcggcacac cgagccgccg cggggcggcg agagatcgtc tccgacgcgc
     8941 gcgggctgcg ccgcgcccgt tgccggcgtg ctgtcggtcg cccagatcag gctgcacatc
     9001 gcgccgggct ggaacggatc ggcgcgcagg tcgggcgcgg gaccgtcggc gatcgcgcgc
     9061 gagcggccgc gctcgtcgtc gagcgtcacc acgcgccgca ccgccagggc gtcgatcacg
     9121 gcagcttcgc ccccatcatc acgaacgcca tcaggctcgg cccgcgcgga ttcgtccagc
     9181 cgtgccagtt gccgagctgc accacgacgt cgcccggctt catcacgcgc tcgccgtcgt
     9241 cgagccgcag cacgcgctcg ccctcgagca cgatgccgta gtcgacggtg tcggacttgt
     9301 ggatcggcga gctgaaagcg ttctgcccgc ccttgtccca ggtgccgccg ggacggcgcc
     9361 gcggctcgtg cacgggcacc gccgacgggt ccttcgccgc gtcgtattcc gcgggcttgc
     9421 ccgccgattg cacgatgcgc aggtgcccgc ccgattcggg cggctcgaaa tggaacggaa
     9481 gattgccgca gtcgcgctcg cccgagacgt cgaccgggca cgactcgaac acccagagat
     9541 cggtcatcgc cgcgctcgcc gcgaccgcgc cgggattgac gttcggcgcc gggccgtcga
     9601 agagcacgcg cgagcgcccc tgcgcgtcgt tgcccgtgac cacgcgccgg atcggcttca
     9661 cgccctgctt catcgccccg ctccttttgg agaacgaata tacttcgctt ccccgaaccg
     9721 cgcccgcccg cacgccgatg agactctgcc gcttcgacaa gaaccgtctc ggcatcgttc
     9781 agggcggcga ggtgctcgac gtcacgaagg cgctcgacgt catcccggag cagcgctggc
     9841 cgctcgcgca gggcgacccg ctgatcctca acctgaagaa agtgatcgcc gcggcgaaga
     9901 agctcgcgcc gcgcgcgaag cgcaagccgc tcgcgaaggt gaagctgctc tcgccggtcg
     9961 cgaacccggg caagatcatc gccgcgccga tcaactacaa cgaccacatc gccgagtcga
    10021 tcaaggaccc gggcatcgcg cacggccgca ccaacatcgc gaagggcatc ggcgactggg
    10081 ggctctttct caaggcgggc tcgtcgctga tcggtttcgg cgaggagatc cggctgcgct
    10141 ggcccgagcg gcgcaacgac cacgaggtgg agctcgcggt gatcatcggc aagaaaggga
    10201 acaggatccc gcgcgaccgg gcgatggact acgtctgcgc ctactcgatc gggctcgaca
    10261 tgacggtgcg cggccccgag ctgcagtgct tccgcaagtc gatcgacacc tactcggtgc
    10321 tcgggccgtg gctggtgacc gccgacgaga tcgccgaccc gaacgcgctc gacctctcga
    10381 tcgccgtgaa cggcgagctg cgccagaatt cgaacacgag gtacctcgtc tacgacgtgc
    10441 agcgcctgat cgagttcggc tcggcgatgt acacgctgca cccgggcgac gtgatcatga
    10501 ccgggacgcc ggcgggggtg tcgccggtga agccgggcga ccgcctgcac gcgaaggtcc
    10561 agggcgtagg cgaggccgac atccggatcg cggcggagta cgcctaggag gccgcatggc
    10621 ccaggacgac aagctccagc tcacgagcga cctgcgcgtc gcgctgcagg agttctactt
    10681 ccgctacgcc gagtgcctcg acggcgggcg cctcgagcag tggcccgagt tcttcctcga
    10741 cgcgggcgcc taccggctca ccacgcggcg cgcgctgcgg ctggggcccg acgacgacat
    10801 cttttcgctc aggaccaagt cggcgctgcg cgaccgcatc gtcgcgatcc agcagtccga
    10861 ggactacgag ccgcacacgc agcgccactt catctccaac ttccgcgtcc aggtctcggg
    10921 cgcggacgag ctgcgcgtgc aggcgaacgt ccaggtcctg cgcacctacc cggggcgcga
    10981 gaccgaggtg ctgtgctcgg gccagtacgc cgaccgcgtc gccagcgcgg ccgggcgctt
    11041 caacttccgc gagaagtgct gcgtgctcga ctccgacatc gcgccgcgcg acctgattta
    11101 tcccgtttga tcggcatttg atctgtattt cggtttgacg aaggttcggg ttttccccta
    11161 aaatttcgcg gttttgcagc ttcggggtgg gcgtgagcgg gcagaagagc cggccgagcg
    11221 acccggtgct ggaccaggtg taccggatca tcgggctgca ggttctcgtc accgcgctga
    11281 tatccctgcc gctcctcgcg atctttggcg cgcgggtcgc cgcttccgct ttcgcgggcg
    11341 gggcgatcgg cttcacgacc gcttgggtct acgccaggag aatgaccgcc gcctctcgca
    11401 cgggcgggcg gtccgacgat ccccggatcc tgctccggaa ccacttcgcg gccgagctgc
    11461 tgaagctggg gctcaccgtg gtcctgctcg cggcggtgct catcttcttc ccggaggtgg
    11521 ccacgctgcc gctgctgctg acgttcatgg caacgctggc cgtctactgg gtggcgttgc
    11581 tgtttgtttt ttgaggaaag agaaaaagcc tacatggccg agagcgcggc gccgctgagc
    11641 gccaacgagt acatcgggca tcaccttcag aacctcacgg tcggaagcgg cttctggact
    11701 ctccacgtcg acacgctgct gatgtcgggg ctggtcggca tcgtcgtctt cggcctgatg
    11761 tactgggccg cgcgcagcgc gaggaacggc gtccccggca agctgcaggc cttcgtcgag
    11821 atcgtcgtcg gcatggtcga cgagcaggtg cgcgacacct tccacgcgga aagcgcgctg
    11881 gtcacgccgc tcgcgatcat catcttcgtc tgggtcttct gcatgaacgc gatcgacctg
    11941 atcccggtcg acttcatccc gggcttcgtc gagcacacgt tccacgtcga gcatttccgg
    12001 ccggtgccga ccgccgaccc gaacgcgacc ttcgcgatgt cgctcacggt gttcctgctg
    12061 atctgggtgt tcaacatccg ctacaagggc ttcgtcgggc tgctgcgcga gatgctcacc
    12121 gcgcccttcg ggccgtggct cgcgccggtc aacttcatcc tgcggctggt cgaggagttc
    12181 gccaagcccg tgtcgctcgc gctgcggctg ttcggcaaca tgttcgccgg cgaggtgctg
    12241 ttcatcctga tcgtgctgct cgccggcgta tggagcggcc cgaacgtcgg cagcgcgtcg
    12301 tcgttcctcg gccacgtcgt gctctcgctc ggctgggcga tcttccacat cttgatcatc
    12361 acgctccagg ccttcatctt catgatgctg accatcgtgt acctgtcgct ggcgagcgaa
    12421 tcgcattgac cctttttcta gactaggcaa accaggagaa aaaaatggaa gcattagcgc
    12481 tgttcttcgg acagacccag atcgccgccg gcctgctgat cggcatgggc gcgctcggca
    12541 ccgcgatcgg gttcggcatc ctcggaggac ggttcctcga aggcgccgcg cgccagccgg
    12601 agatgatccc gacgctgcag gtgaagatgt tcatcgtcgc cggcctgctc gacgcgttca
    12661 cggcgatcgc gaccgccatt gcggtgctgc tgctctacgc caacccgctg atcggcgcgg
    12721 tcaagggcgg ctgagcggtc ccgcagggag agggcagtga acgtcaacgc aacactgatc
    12781 ggccaggtga tctggttcgc cctgttcatc tggttctgca tgaagttcgt gtggccgtat
    12841 ttccagcgca tcatcgccga gcgccagagg acgatcgcgg aagggctggc cgccgccgag
    12901 aagggccgcc agtcgctgct cacgtcggcg cagcaggccg aggccgagct gcgcggggcg
    12961 cgcgagcgcg ccgccgagat cgtcgcgcag gccgagaagc gcgccgcgca gctagtcgac
    13021 gaggcgcgca ccgccgcccg cgacgagggc agccgcgaga aggccgccgc gaaggccgag
    13081 atcgaccagg aagtgtcgcg cgcgcgcgag acgctgcgcg agcaggtcgc ggcgctcgcc
    13141 gtcgccggcg ccgagaagat cctgcgccgc gaggtgaacg cccaggcgca cgccgagctg
    13201 ctcgcgcagc tgaagaagga gatctagtgg ctgaaaccgc gacgatcgcg cgcccgtacg
    13261 ccgaggccgt gttcggcctc gccgacaaga cgggcgcgct ggcgaagtgg tcgcagctgc
    13321 tcgccgcgat ggcctcgtcg gcggcggatc cgcgggtccg ggcgctgatc ggcgacccga
    13381 aggtgcgcga ggaccagctc tacgggctga tcgccgcgct cgccggcgcc gacatcccgg
    13441 ccgaggcgca gaacctggtg cgcgtgctga tggcgaacga ccgcctgacg ctgctcccgg
    13501 agatcagcgc gcagttcgag tcgctgcggc acgagcgcga aggcgtggtc gaggccgaga
    13561 tccgcaccgc tttcccgatg gcgaacggcc agctggaagg cctcgtgggc gacctcgagc
    13621 gccgcttcaa gcgcaagatc gacgccaagg tcgtcgtcga caaggagttg atcggcggcg
    13681 tgcgcatcgc cgtcggcgac gaggtcatcg acggctcggt gcgcggcaag ctcgccgaca
    13741 tggcaagcgc gctcaagtct taacgcacta cacagaatcc agcaggagct tccatgcaac
    13801 aactgaaccc ctccgaaatc agcgagctga tccgcgcgaa gatccagaac ctcgcgctct
    13861 cggccgacgt gcgcacgcaa gggacggtgg tgaccgtcac cgacggcatc tgccgcatcc
    13921 acgggctgtc cgacgcgatg cagggcgaga tgctcgagtt cccgggcaac accttcggcc
    13981 tcgcgctcaa cctcgagcgc gactcggtcg gcgcggtggt gctcggcgcc tacgagcaca
    14041 tcaccgaggg cgacacggtg aagtgcaccg ggcgcattct cgaggtgccc gtggggcccg
    14101 agctgctcgg gcgcgtggtg aactcgctcg gccaggcgat cgacggcaag ggcgcgatcg
    14161 ccacccagcg ctccgagccg atcgagaaga tcgcgccggg cgtgatccac cgcaagtccg
    14221 tgtcgcagcc ggtgcagacg ggcctcaagg cgatcgacgc catggtgccg gtcggccgcg
    14281 gccagcgcga gctgatcatc ggcgaccggc agacgggaaa gaccgcggtc gcggtcgaca
    14341 ccatcatcaa ccagaagggc aaggacctga tctgcgtcta cgtcgcgatc gggcagaagg
    14401 cctcgaccat cgccaacgtc gtccggaagc tcgaggagta cggcgcgatg gcgtacacga
    14461 tcgtggtcgc ggcgtcggcg tcggagtcgg cggcgatgca gtacatcgcg ccctacgcgg
    14521 gctgcaccat gggcgagtac ttccgcgacc gcggccagga cgcgctcatc atctacgacg
    14581 acctgaccaa gcaggcgtgg gcgtaccggc agatctcgct gctgctgcgc cgcccgccgg
    14641 gccgcgaggc gtacccgggc gacgtcttct acctgcactc gcgcctgctc gagcgcgccg
    14701 cgcgcgtgaa cgaggagtac gtcgagaagt acacgggcgg cgaggtgaaa ggcaggaccg
    14761 ggtcgctcac ggcgctgccg atcatcgaga cgcaggcggg cgacgtgacc gcgttcgttc
    14821 ccacgaacgt catctcgatc accgacggcc agatcttcct cgagaccgac ctgttcaacg
    14881 ccggcatccg tcccgcgatc aacgcgggca tctcggtgtc gcgcgtcggc ggcgcggcgc
    14941 agaccaagat catgaagagg aaggacacgg gcggcggcgt gcgcctcgcg ctcgcgcagt
    15001 accgcgagct cgcggccttc gcgcagttcg cctccgacct cgacgaggcg acccgccggc
    15061 agctcgagcg cggccgcatg gtcaccgagc tgatgaagca gccgcagtac cagccgctcc
    15121 aggtctggga gatggcgctg acgctgttcg cggtcaacaa cggctacttc gacgacatcg
    15181 acgtcaggaa ggcgctcgcc gccgagcgct cgatgcgcga ctacctgaag accaagtacg
    15241 cgccgctcgt gaagagcatg gaggacaaga aggacctccc ggcggacgcc gagaaggcgc
    15301 tgaagagcgc gatcgaggac tggaagaaga gcggcagctt ctgatgccag gaaccaagga
    15361 aatccggatg aagatccgga gcgtgcagaa cacgcgcaag atcaccaagg cgatggagat
    15421 ggtggccgcg tcgaagatgc gcaaagccca ggaccgcatg cgccacgcgc gcccgtacgg
    15481 cgacaagatc cgcaacgtcg ccgcgcacat cagccacgcg aaccccgagt accgccaccc
    15541 gttcctggtc gagcgcgaca cggtgaaggg cgtcggcatc atcgtcgtca ccaccgacaa
    15601 ggggctgtgc ggcgcgctga acaccaacct gctgcggctg gccctcacac agtacaaggc
    15661 atggcaggcg cagggcgaga aggtcgaggt gtgcgcgatc ggcggcaagg gcttcggctt
    15721 catgcagcgc ctgggcgcga acgtcgtctc gcacgtggtg cagctcggcg accggccgca
    15781 catggacaag ctgatcggcg cggtgaaggt gatgctcgac ggcttcgtcg ccgaccgctt
    15841 cgacaagctg atgctcttct acacgaagtt catcaacacc atgaagcagg agccggtgat
    15901 ggagcagctg ctgccgctca ccggcgagaa gatcggggtg ccgcagggcg cgtgggacta
    15961 catctacgag cccgaggcgc gcgcggtgct cgaccaggtg ctgacgcgct acatcgaggc
    16021 gatcatctac caggcggtgt ccgagaacat ggcctccgag cagtcggcgc gcatggtcgc
    16081 gatgaaggcc gcctcggaca acgccgccac cctgatcgac gagctgacgc tcatctacaa
    16141 caagaaccgg caggcggcga tcaccaaaga gatctctgaa atcgtgggcg gcgcggccgc
    16201 cgtttgagga aaaccatggc agaagcacaa agcatcggaa ccatcgtcca gtcgatcggc
    16261 gcggtgatcg acatcgaatt cccgcgcgag gcgatgccca gggtctacga cgcgctcgtg
    16321 ctcgaggaca gcgggaagag cttcgcggag aaggggctca ccttcgaggt cgagcagcag
    16381 ctcggcgacg gcatcgtgcg ctgcatcgcg ctcggctcct ccgacggcct gcgccgcggc
    16441 atgaaggtga agagcaccgg agcgccgatc tcggtgcccg tcggccaggg cacgctcggg
    16501 cgcatcatgg acgtgctcgg gcgcccgatc gacgaggcgg ggccgatcca gtcgaaggag
    16561 aagcgctcga tccaccaggc cgcgccgaag ttcgacgagc tcagcccttc ggtcgagctg
    16621 ctcgagaccg gcatcaaggt catcgacctg atctgcccgt tcgcgaaggg cggcaagatc
    16681 gggctgttcg gcggcgcggg cgtgggcaag acggtgaaca tgctcgagct gatcaacaac
    16741 atcgccacgc agcactcggg cctgtcggtg ttcgccggcg tcggcgagcg cacgcgcgag
    16801 ggcaacgact tctaccacga gatgatcgag gccgaggtca tcaacctgaa gaacatcacc
    16861 gagtcgaagg tggcgatggt gttcgggcag atgaacgagc cgccgggcaa caggctgcgc
    16921 gtcgggctga ccggcctcac catggcggag tacttccgcg acgagggccg cgacgtcctg
    16981 atgttcatcg acaacatcta ccgcttcacg ctcgcgggga cggaagtctc ggcgctgctc
    17041 ggccggatgc cctccgcggt cggctaccag ccgacgctcg cggaggagat gggcaagctc
    17101 caggagcgga tcacgtccac caagaccggc tcgatcacgt cgatccaggc ggtgtacgtg
    17161 cccgcggacg acctgaccga cccgtcgccc gcgacgacct tcggccacct cgacgcgacc
    17221 gtcgtgctct cgcgcgacat cgcggcgctc gggatctacc cggcggtcga cccgctcgac
    17281 tcgacctcgc gccaggtcga cccgaacacg atcggcgagg accactacaa cacgacgcgc
    17341 gcggtgcagg cgacgctgca gcgctacaag gagctgcgcg acatcatcgc gatcctgggc
    17401 atggacgagc tctcgcccga ggacaagctc gcggtctcgc gcgcccgcaa gatccagagg
    17461 ttcctctcgc agccgttcac ggtggcgcgc aacttcaccg gccaggaagg caagtacgtc
    17521 ccgctcaagg acacgatcaa gggcttcaag atgatcgtga acggcgagtg cgacgccctg
    17581 cccgagcagg cgttctacat ggtcgggccg atcgaggaag cgttcgccaa ggccgaggag
    17641 atgaagaaag agacggcgaa agcgtaatgg ccgaagccca caccatccac gtcgacgtcg
    17701 tcagcgccga agagagcatt tactccggcg aggcgacgtt cgtcgccctc ccgggcgagg
    17761 cgggcgagct cggcatctat ccgcgccata cgccgctcat cacgcggatc aagccgggat
    17821 cggtccggat ccagccggcg ggcgggggcg aggaggagct gatcttcgtc cagggcggga
    17881 tcctcgaggt ccagcccggg gtggtcacgg tgctcgccga cacggcgatc cggggccacg
    17941 acctggacga ggccaaggcg accgaggcgc tggcccgcgc cgaggaagcg agaaaaaacg
    18001 caaaaagcca agaacaagtg gcgacagtcg aggcggaagt tgctacactc gccgcccagt
    18061 tggccgcgat ccgcaagctg cggcgcaaga aataacaaga aacaacaaga aactcgctcg
    18121 cgaacaagaa cagggagaga tcaagaagaa gaacagcgaa agttggaact cgaaaggcgc
    18181 cctccgtggc gcctttttta ttgggcggga ggttgaagat aaaatcccgc cgatgacctg
    18241 gcgcgtcgtc cccatggaag aacagcacat cgagggctac cggtcggcgc tcgacagcgt
    18301 cgcccgcgag cgcaagtacc tcgccttcct cgaggcgccg ccggtcgagg cggtgaccgt
    18361 gttcgtcctg ggcaacctgc gcgcgaaccg gccgcacttc gtcgccgtcg acggcggcga
    18421 ggtggtcggc tggtgcgaca tctcctcgct cgagcgcccg gcgttcgcgc actccgggat
    18481 gctcgggatg ggcgtcctcg cctcgcaccg cgggcgcggc atcggccgcg agctgctgcg
    18541 cgcgacgctc gagaacgcgc acgagatggg gctgacgcgc gtcgagctga ccgtgcgcga
    18601 gccgaacgcg cgcgcccgcg agctctacga gaaggccggc ttcgcggtcg agggcctcaa
    18661 gcgcaacgcc gtgcgcgtcg acgggcgcta cgagaacctc gtgtgcatgg cgatcctgtt
    18721 tcacgagggg cgctagctac gccaggcggc tgggccggat ctccatccac agccgccccg
    18781 gcgtgcgctc gacgcgcgcc gagtccttcg tcgggttcat gaggtacgtc aggtggcaca
    18841 ggtcgcgcac gttcgcgagc gcctcggcgc ggaagagcgc ggcgttgacg ccgcctttcg
    18901 ggcggatgtc gcgcgccgag cgcaccagca ggccgttctg tccctggtcg tgcaggtacc
    18961 ggcccagcgg ctgcgtgaag gcgtagctcg agcggtcgac caggcgcttc tccttcctct
    19021 cccggccgcg gaggtcgatc aggatcgcgt cgcagcgcac ctcgaacacg cggcgctcgt
    19081 tcgcgatggc gcggtcctcg cccgggaacg catcggcgag gaagcggtgg cggtggtgca
    19141 cggtctcgta caccgtggtc tcgagctcga gcgagccgta ccacacgccg taggcgaggc
    19201 cgtcggagaa gcgcgtcgcg tggccgttcc actcgacgaa cgggtacgtg acgacgctgc
    19261 cgtagtcgaa gggccgcgtg atcagcggcg cggcgctcgc gatgcgcgcg acaccctcgg
    19321 ccgcgaccgc gacggcgcgc tcggcgggat cgtccgagag gtcgtcgaac aggtcctgcg
    19381 agacgcggat cgtctggatg ttgcggacga ggtcgccgtg gaagtcctcg acctcgccga
    19441 tgaggtcctc gtacacggca ctaccgtccc cgctcgaaat cgagatagcg ccgcaccgcc
    19501 agcaacccct cgaacccctg ctt
//
LOCUS       DATDMW010000169         7937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1169053, whole genome shotgun sequence.
ACCESSION   DATDMW010000169 DATDMW010000000
VERSION     DATDMW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169053"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VI319_07395"
     CDS             <1..413
                     /locus_tag="VI319_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY6863712.1"
                     /translation="AAMVCGHETAGLSNEDVLKCGRLATIPADAEHSSLNLAQAVQVL
                     AYEVRRASSAGPPARDRLPDYATHEGVEAFYDQLERSLVDAGFLDPANPGRLMERLRR
                     LFGRARLEREEVNILRGMLSAWEPGSPKRKNVRK"
     gene            469..1206
                     /gene="cysE"
                     /locus_tag="VI319_07400"
     CDS             469..1206
                     /gene="cysE"
                     /locus_tag="VI319_07400"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY6863713.1"
                     /translation="MFGRIREDIASIFERDPAARNTWEVLTCYPGLHAVMVHRFAHWL
                     WRHRIRWPARFVSHLGRFLTGIEIHPGANIGRRFFIDHGMGVVIGETAEIGDDCTLYH
                     GVTLGGTTWKRGKRHPSLGNGVVIGAGAKLLGPISVGDRARVGSNAVVVKDVPPGATA
                     VGIPARIMVDEQDRSREEKAATLGFSAYAVSAKAEDDPLAKAVSGLLDHSVDIDQRIA
                     LILEEIESLKRERGETQRQMPRVVNKG"
     gene            1306..1797
                     /gene="iscR"
                     /locus_tag="VI319_07405"
     CDS             1306..1797
                     /gene="iscR"
                     /locus_tag="VI319_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEY6863714.1"
                     /translation="MRLTTKGRFAVTAMLDLALNGGKGPVTLAGISQRQSISLSYLEQ
                     LFGKLRRHTLVESVRGPGGGYTLARGIDKLSVADIVTAVDEPLDATQCGGRENCKDDR
                     RCMTHNLWATLNEKMYEYLDSVKLSDLVAQQAARKDHEHEPKVLQDKRCKESTTASNT
                     VSA"
     gene            1794..3008
                     /locus_tag="VI319_07410"
     CDS             1794..3008
                     /locus_tag="VI319_07410"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEY6863715.1"
                     /translation="MSVKLPIYLDYSATTPVDPRVAQKMIPYLTEHFGNPASRSHAFG
                     WEAEKAVEEARGHVAALLNADPKEIVWTSGATEGNNLALKGAANFNKGKGKHIITQKT
                     EHKAVLDTVRELERQGFEATYLDPEPNGLVDLKKLEAAIRPDTILVSIMMVNNEIGVI
                     QPIAEIGEICRARGIVFHCDAVQAAGKVPIDLQKLKIDLLTVTAHKVYGPKGIGALYV
                     RRKPRVRIEPQNHGGGHERGFRSGTLATHQIVGMGEAFRIARAEMATENERIRMLRDR
                     LLKGIKEIEEIYVNGDLERRIPGNLNVSFNFVEGESLIMAIKDVAVSSGSACTSASLE
                     PSYVLRALGRSDELAHSSIRFTLGRFTTEEEVDYTVDLLKRKIAKLRELSPLWEMHKE
                     GVDLNTVQWAAH"
     gene            3018..3437
                     /gene="iscU"
                     /locus_tag="VI319_07415"
     CDS             3018..3437
                     /gene="iscU"
                     /locus_tag="VI319_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEY6863716.1"
                     /translation="MAYSDKVIDHYENPRNVGSFDKNDEGVGTGMVGAPACGDVMKLQ
                     IRVNEQGIIEDARFKTYGCGSAIASSSLVTEWVKGKSIDEAEKIRNTQIAEELALPPV
                     KIHCSILAEDAIKAAISDYRKKHGLTEPAPVEAKKVA"
     gene            3440..3760
                     /gene="iscA"
                     /locus_tag="VI319_07420"
     CDS             3440..3760
                     /gene="iscA"
                     /locus_tag="VI319_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEY6863717.1"
                     /translation="MITLTEPAATHIKAFLAKRGKGVGLRLGVRTSGCSGLAYKLEYA
                     DVVNPEDKTFESRGIKIVVDPKSLAFLDGTELDFAREGLNEGFKFRNPNVKDQCGCGE
                     SFNV"
     gene            3834..4352
                     /gene="hscB"
                     /locus_tag="VI319_07425"
     CDS             3834..4352
                     /gene="hscB"
                     /locus_tag="VI319_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEY6863718.1"
                     /translation="MNQSQTHFELFGLAAGFDIDLAALESRYRQLQREVHPDRFAAAP
                     QAQQRVAMELATRVNDAYRTLKSPVERAKYLLQLHGVDPELETNTAMPHDFLVQQMEL
                     REALEQAVDAENGEKLADLRDRLQGGRGALLLEIEAQLGARDWNGAAGTLRRLMFMEK
                     LGEEIAAAEDDL"
     gene            4353..6215
                     /gene="hscA"
                     /locus_tag="VI319_07430"
     CDS             4353..6215
                     /gene="hscA"
                     /locus_tag="VI319_07430"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEY6863719.1"
                     /translation="MALLQISEPGQSPAPHEHRLAVGIDLGTTNSLVATVRNGIAVVL
                     NDREGRALLPSIVRYRPDGVEVGHAAEARQARDPRNTIVSVKRFMGRGLKDVTYVENS
                     PYQFVDSPGMVQIRTVAGVKSPVEVSAEILKVLRGRAEESLSGPLTGAVITVPAYFDD
                     AQRQATKDAGRLAGLNVLRLLNEPTAAAIAYGLDNASEGIYAVYDLGGGTFDLSILRL
                     SKGVFEVMATNGDAALGGDDFDHRLFCWVVEEAKLAPVSDEDTRLLLVKSRAAKEFLT
                     LHDEARITASLGSGETVDVTITRRRFHEMTKHLVAKTLGPTRKALRDAKLDVSEVKGV
                     VMVGGATRMPHVQAAVAELFRQAPLNNLDPDKVVALGAAIQANVLVGNRRPGDDWLLL
                     DVIPLSLGIETMGGLVEKVVPRNSTIPVARAQEFTTFKDGQSAMAVHVLQGERELASD
                     CRSLARFELRGIPPMPAGAARIKVTFQVDADGLLAVSAREATTGVEASVAVKPSYGLT
                     DEQVTRMLRDSIEHADADVHRRQLLERRVEGERMAAAVRAALQEDPDLVEAGERPGID
                     AALAALEDAIAGTDAQVIKIAIERANRATEPFAARRMDRSIRRALAGKSVADIR"
     gene            6215..6553
                     /gene="fdx"
                     /locus_tag="VI319_07435"
     CDS             6215..6553
                     /gene="fdx"
                     /locus_tag="VI319_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEY6863720.1"
                     /translation="MPKLTVLPHAALAPEGRVIEAKEGVSICDTLLENGVEIEHACEK
                     SCACTTCHVIVRKGFDSLDPSEEREDDLLDKAWGLEAQSRLSCQAVVQDEDLTIEIPK
                     YTINQVSESK"
     gene            6610..6804
                     /gene="iscX"
                     /locus_tag="VI319_07440"
     CDS             6610..6804
                     /gene="iscX"
                     /locus_tag="VI319_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEY6863721.1"
                     /translation="MKWTDTQDIAIALVDKYPDVDPKTIRFTDLFKWVQELDDFADDP
                     NRCGEKILEAIQMAWIDEAE"
     gene            6852..7118
                     /locus_tag="VI319_07445"
     CDS             6852..7118
                     /locus_tag="VI319_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY6863722.1"
                     /translation="MCRSIKTLFNFQPPVTDEEIRSASLQFVRKLSGFHKPSKANEAA
                     FALAVDEVAAVSKKLLVSLETSAPPRNREEEAAKARARAAQRFA"
     gene            7126..>7937
                     /locus_tag="VI319_07450"
     CDS             7126..>7937
                     /locus_tag="VI319_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6863723.1"
                     /translation="MTPGLSYALGALVLYGLADFVYKRAAAAGAQPHRFLMVQTWFFT
                     PLAILYGAATGTLAYSRGAAWGLVAGVLAVVGYYNFAWSLRHGAVSTNATIFRLSFVV
                     TAALGIALLGEPLTAPKTAGLALALVAVWLLVARPGAAGATAGGERAASLARVLVATV
                     SVGVLSFLYKLGLRLGATPITMVASQGVVAVSVSTLFTAYTERDIRPSAVALRFAPVS
                     AILLVVAFVLLAKGLQAGEASVVAPVAQMGFAVTAILGFVVLREPVTARRVAG"
BASE COUNT         1374 a         2661 c         2754 g         1148 t
ORIGIN      
        1 atgcggccat ggtctgcggc cacgagaccg cgggcctctc gaacgaggac gtgctcaagt
       61 gcggccgcct cgcgaccatc ccggcggatg cggagcattc gtcgctcaac ctcgcccagg
      121 ccgtgcaggt cctggcctac gaggtgcggc gcgcgtccag cgcgggcccg ccggcgcggg
      181 accggctgcc cgactacgcc acccacgagg gcgtcgaggc cttctatgac cagctcgagc
      241 gcagcctggt cgacgcgggt tttctcgacc ctgcgaatcc cgggcgtttg atggagcggc
      301 tgcggcgcct cttcgggcgt gcgcggctgg agcgcgagga ggtcaacatc ctgcgcggca
      361 tgctgtccgc atgggagccg gggtcgccca agcgaaagaa cgtccgaaaa tagttgacca
      421 aatcgttagg ggtttcccat aatgccggat tcgaatggga aaaccggcat gttcggcagg
      481 atccgcgagg acatagcgag catcttcgag cgcgacccgg cggcgcgaaa cacctgggag
      541 gtgctgacgt gctacccggg cctgcacgcg gtcatggttc atcgcttcgc gcactggctc
      601 tggcgccacc gcatccgctg gcccgcgcgc ttcgtgtcgc acctcgggcg ctttctcacc
      661 gggatcgaga tccacccggg cgcgaacatc ggccggcgct tcttcatcga tcacggcatg
      721 ggcgtcgtca tcggcgagac cgccgagatc ggcgacgact gcacgctcta ccacggcgtg
      781 acgctcggcg ggacgacctg gaagcgcggc aagcgccacc cgagcctcgg caatggcgtg
      841 gtgatcggcg ccggggcgaa gctgctcggg ccgatcagcg tcggcgaccg cgcacgcgtg
      901 ggctcgaacg ccgtcgtggt gaaggacgtg ccccccggcg ccaccgcggt cgggatcccg
      961 gcccggatca tggtcgacga gcaggatcga tcgcgcgagg agaaggccgc cacgctgggc
     1021 ttttcggcct atgccgtcag cgcgaaggcc gaggacgatc cgctggccaa ggccgtttcc
     1081 ggtctgctcg accactcggt cgacatcgac cagcgcatcg ccctgattct cgaggaaatc
     1141 gagagcctga agcgcgagcg gggcgagacc cagcggcaga tgcctcgggt cgtgaacaag
     1201 ggttgacctg aaacaaaggt tgatcggatt tgtcggatat cctatacttg agcgtattcc
     1261 tcaggtatta gcctgaccta cgcaaggggt cccggagcgc caacgatgag actcaccacc
     1321 aaaggacgtt tcgccgtcac cgccatgctc gatctcgccc tgaacggcgg gaaagggccg
     1381 gtgacgctgg ccggcatcag ccagcgccag agcatctcgc tgtcgtacct cgagcagctg
     1441 ttcggaaagc tgcgccggca cacgctcgtc gagagcgtgc gcggcccggg cggcggctac
     1501 acgctcgcgc gcgggatcga caagctctcg gtcgccgaca tcgtgaccgc ggtcgacgag
     1561 ccgctcgacg ccacgcagtg cggcggccgc gagaactgca aggacgaccg ccgctgcatg
     1621 acgcacaacc tgtgggcgac gctgaacgag aagatgtacg agtacctcga ctcggtgaag
     1681 ctgtccgacc tggtggcgca gcaggcggcg cgcaaggatc acgagcacga gcccaaggtg
     1741 ctgcaggaca agcgctgcaa ggaaagcacg accgcttcga atacggtgtc ggcatgagcg
     1801 tgaagcttcc catctatctc gactactccg cgaccacgcc ggtcgatccg cgcgtggcgc
     1861 agaagatgat cccgtacctg accgagcact tcggcaaccc ggcgagccga tcgcacgcat
     1921 tcggctggga ggcggagaag gcggtcgagg aggcgcgcgg ccacgtcgcc gcgctgctca
     1981 atgccgatcc caaggagatc gtctggacct cgggcgcgac ggagggcaac aacctcgcgc
     2041 tgaagggcgc cgcgaacttc aacaagggca agggcaagca catcatcacg cagaagaccg
     2101 agcacaaggc cgtgctcgac acggtgcgcg agctcgagcg ccagggcttc gaggcgacct
     2161 acctcgaccc ggagcccaac ggcctcgtcg atctgaagaa gctggaagcc gcgatccgcc
     2221 cggacacgat cctcgtctcg atcatgatgg tgaacaacga gatcggcgtg atccagccga
     2281 tcgccgagat cggcgagatc tgccgcgccc ggggcatcgt cttccattgc gacgcggtgc
     2341 aggccgcggg caaggtgccg atcgacctgc agaagctgaa gatcgacctg ctcacggtga
     2401 ccgcgcacaa ggtctacggg ccgaagggca tcggcgcgct ctatgtgcgc cgcaagccgc
     2461 gcgtgcgcat cgagccgcag aaccacggcg gcgggcacga gcgcggcttc cgctcgggga
     2521 cgctggcgac gcaccagatc gtgggcatgg gcgaggcctt ccgcatcgcc agggccgaga
     2581 tggcgaccga gaacgagcgc atccgcatgc tgcgcgacag gctgctgaag ggcatcaagg
     2641 aaatcgagga gatctacgtc aacggcgacc tcgagcggcg catcccgggc aacctcaacg
     2701 tcagcttcaa cttcgtcgaa ggcgaatccc tgatcatggc gatcaaggac gtcgccgttt
     2761 cttcgggatc cgcgtgcacc tcggccagcc tcgagccgtc ctacgtgctc cgcgcgctgg
     2821 gacgcagcga cgagctggcg cacagctcga tccggttcac gctcggccgc ttcacgaccg
     2881 aggaggaggt cgactacacg gtcgacctgc tcaagcgcaa gatcgccaag ctgcgcgagc
     2941 tgtcgcccct ctgggaaatg cacaaggaag gcgtcgactt gaataccgtg cagtgggctg
     3001 cacattgagg aattgacatg gcatacagcg acaaggtcat cgatcactac gagaacccgc
     3061 gcaacgtcgg ctcgttcgac aagaacgacg agggcgtcgg cacgggcatg gtcggcgcgc
     3121 cggcctgcgg cgacgtcatg aagctgcaga tcagggtgaa cgagcagggc atcatcgagg
     3181 acgcgcgctt caagacctac ggctgcgggt cggcgatcgc gtcgtcctcg ctcgtcaccg
     3241 agtgggtgaa gggcaagtcg atcgacgagg ccgagaagat ccgcaacacg cagatcgccg
     3301 aggagctcgc gctcccgccg gtgaagatcc actgctcgat cctcgccgag gacgcgatca
     3361 aggcggcgat ctcggattac cgcaagaagc acggcttgac cgagcccgct ccggtcgagg
     3421 ccaagaaggt ggcctaggga tgatcacgct caccgagcct gccgcgaccc atatcaaggc
     3481 cttcctcgcc aagcgcggga agggcgtggg actgcgcctg ggcgtgcgca cctcgggctg
     3541 ctcggggctc gcgtacaagc tcgagtacgc cgacgtcgtg aaccccgagg acaagacctt
     3601 cgagagccgg gggatcaaga tcgtcgtcga cccgaagagc ctcgccttcc tcgacggcac
     3661 cgagctcgat ttcgcgcgcg agggcctgaa cgagggcttc aagttccgca acccgaacgt
     3721 gaaggaccag tgcgggtgcg gggagagctt caacgtatga cgtcatcccc gcgcaagcgg
     3781 ggatccagcg acgtcttgat gaaaaggggc gcaagcccct tttttgcacc cggatgaacc
     3841 agagtcagac ccacttcgag ctcttcggcc tcgccgcggg cttcgacatc gacctcgcgg
     3901 cgctggagtc gcgctatcgc cagctgcagc gcgaagtcca tcccgaccgc ttcgccgccg
     3961 cgccgcaggc gcagcagcgc gtggccatgg agctggcgac gcgggtgaac gacgcgtacc
     4021 ggacgctcaa atcgccggtg gagcgcgcga agtacctgct gcagctgcac ggcgtggatc
     4081 ccgagctgga gaccaatacc gccatgccgc acgacttcct cgtgcagcag atggagctgc
     4141 gcgaggcgct cgagcaggcg gtcgacgccg agaacggcga gaagctcgcg gacctgcgcg
     4201 accggctgca gggcggccgc ggcgcgctgc tgctcgagat cgaggcgcag ctcggcgcca
     4261 gggactggaa cggggcggcg ggcacgctgc ggcggctgat gttcatggag aagctcggcg
     4321 aggagatcgc cgcggccgag gacgacctct agatggcgct gctccagatc tccgagccgg
     4381 gccagtcgcc ggcgccgcac gagcaccgcc tcgcggtcgg catcgacctc ggcacgacca
     4441 actcgctggt cgcgacggtg cgcaacggca tcgccgtggt gctgaacgac cgcgagggac
     4501 gcgcgctgct gccgtcgatc gtgcgctacc gtcccgacgg ggtcgaggtg ggacacgcgg
     4561 ccgaggcccg gcaggcgcgc gacccgcgca acacgatcgt gtcggtgaag cgcttcatgg
     4621 ggcgcggcct gaaggacgtc acctacgtcg agaacagccc gtaccagttc gtcgattcgc
     4681 cggggatggt gcagatccgc accgtcgccg gggtgaaatc gccggtcgag gtctcggccg
     4741 agatcctgaa ggtgctgcgc gggcgcgccg aggagtcgct ctcgggcccg ctgacgggtg
     4801 cggtgatcac ggtgccggcc tacttcgacg acgcgcagcg ccaggcgacg aaggacgcgg
     4861 ggcgcctcgc gggcctgaac gtgctgcggc tgctgaacga gccgaccgcg gccgcgatcg
     4921 cctacgggct ggacaacgcc tcggagggca tctacgccgt ctacgacctg ggcggcggca
     4981 ccttcgacct ctcgatcctg cgcctctcga agggcgtgtt cgaggtgatg gcgacgaacg
     5041 gcgacgccgc gctcggcggc gacgacttcg accaccgcct gttctgctgg gtggtcgagg
     5101 aggcgaagct cgcgcccgtc tcggacgagg acacgcgcct gctgctggtg aagtcgcgcg
     5161 cggcgaagga gttcctgacg ctgcacgacg aggcacgcat cacggcatcg ctcgggagcg
     5221 gcgagacggt cgacgtgacg atcacgcgcc gccgtttcca cgagatgacg aagcatctcg
     5281 tcgccaagac gctcggcccg acgcgcaagg cgctgcgcga cgcgaagctc gacgtgtcgg
     5341 aggtgaaagg cgtggtgatg gtcggcggcg cgacgcgcat gccgcacgtg caggccgcgg
     5401 tcgccgagct gttccgccag gcgccgctca acaatctcga tcccgacaag gtggtggcgc
     5461 tcggcgccgc gatccaggcg aacgtgctgg tcggcaaccg ccggcccggc gacgactggc
     5521 tgctgctcga cgtgatcccg ctctcgctcg gcatcgagac catgggcggc ctggtcgaga
     5581 aggtcgtgcc gaggaactcg acgatcccgg tcgcgcgcgc gcaggagttc acgaccttca
     5641 aggacgggca gagcgcgatg gcggtccacg tgctgcaggg cgagcgcgag ctcgcgtccg
     5701 actgccgctc gctcgcgcgc ttcgagctcc gcggcatccc gccgatgccc gcgggcgcgg
     5761 cgcgcatcaa ggtcaccttc caggtggacg ccgacggcct gctcgcggtg tcggcgcgcg
     5821 aggccaccac cggtgtcgag gcctcggtcg cggtcaagcc gtcctacggc ctcaccgacg
     5881 agcaggtgac gcgcatgctg cgcgactcga tcgagcacgc cgacgccgac gtgcaccggc
     5941 gccagctgct cgagcggcgc gtcgaaggcg agcgcatggc ggccgcggtg cgcgcagcgc
     6001 tgcaggagga cccggacctg gtcgaggccg gggagaggcc cggcatcgac gccgcgctcg
     6061 cggccctcga ggacgcgatc gccggcaccg atgcgcaggt catcaagatc gcgatcgagc
     6121 gcgccaaccg cgccaccgag cccttcgcgg cgcgccgcat ggaccggtcg atccggcgtg
     6181 cgctcgccgg caagagcgtc gccgacatcc gctgatgccg aagctcaccg tgctgccgca
     6241 cgccgcgctc gcgcccgaag gcagggtcat cgaggcgaag gagggcgtct cgatctgcga
     6301 caccctgctc gagaacggcg tcgagatcga gcacgcctgc gagaagtcgt gcgcgtgcac
     6361 cacctgccac gtgatcgtgc gcaagggctt cgactcgctc gacccgtcgg aggagcgcga
     6421 ggacgacctg ctggacaagg cctgggggct cgaggcgcag tcgcggctct cgtgccaggc
     6481 ggtggtgcag gacgaggacc tcaccatcga gatcccgaag tacaccatca accaggtgtc
     6541 agaaagcaaa tagcttgtca tcctgagcga agcgaaggat ctgcttttcg gaagggaggc
     6601 gcgaccgaca tgaagtggac cgacacccag gacatcgcca tcgccctggt cgacaaatat
     6661 ccggacgtcg atcccaagac gatccggttt acggacctgt tcaaatgggt ccaggagctc
     6721 gacgatttcg ccgacgatcc gaaccgctgc ggcgagaaga tcctcgaggc gatccagatg
     6781 gcgtggatcg acgaggcgga atgaaagaag gcaatcctca cttcgataaa gatcatcgac
     6841 aagtaaccat catgtgccga agcatcaaga ctctgttcaa tttccagcct cccgtcaccg
     6901 acgaggagat ccgatcggcc tctctgcagt tcgtgcgcaa gctctcgggc ttccacaagc
     6961 cgtcgaaggc gaacgaggcg gcgtttgctc ttgccgtcga cgaggtcgcg gcggtgtcga
     7021 agaagctgct cgtctcgctc gaaacgagcg cgccgccgcg caaccgcgag gaggaggcgg
     7081 cgaaggcgcg ggcgcgcgcg gcgcaacgct tcgcctgagc gccgcttgac cccgggcctg
     7141 tcctacgcgc tcggcgcgct cgtcctctac ggcctcgccg atttcgtcta caagcgcgcc
     7201 gccgcggcgg gcgcgcagcc gcaccggttc ctcatggtgc agacgtggtt cttcacgccg
     7261 ctcgcgatcc tctacggcgc cgcgacgggc acgctcgcgt actcgcgcgg cgcggcctgg
     7321 gggctggtcg cgggcgtgct cgccgtcgtc ggctactaca acttcgcctg gagcctgcgc
     7381 cacggcgcgg tcagcaccaa cgcgaccatc ttccggctga gcttcgtcgt gaccgcggcg
     7441 ctcgggatcg cgctgctcgg cgagccgctg accgcgccga agacggcggg actcgccctg
     7501 gcgctcgtcg cggtctggct gctggtcgcg cggcccggcg ccgcgggcgc gaccgcaggc
     7561 ggcgagcgcg ccgcgtcgct cgcccgcgtg ctggtcgcca cggtgtcggt cggcgtcctg
     7621 agcttcctgt acaagctcgg actgcgcctg ggcgcgaccc cgatcacgat ggtcgcctcg
     7681 caaggcgtcg tcgcggtctc ggtgtcgacg ctgttcaccg cgtacaccga gcgcgacatc
     7741 cggccgtcgg cggtggcgct gcggttcgct ccggtcagcg cgatcctcct cgtcgtcgcg
     7801 ttcgtccttc ttgccaaagg actccaggcc ggcgaggcga gcgtggtcgc gccggtggcc
     7861 cagatgggct tcgcggtcac ggcgatcctc ggcttcgtcg tcctgcgcga gccggtcacc
     7921 gctcgccgcg tcgccgg
//
LOCUS       DATDMW010000170         5431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1418535, whole genome shotgun sequence.
ACCESSION   DATDMW010000170 DATDMW010000000
VERSION     DATDMW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418535"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VI319_07455"
     CDS             <1..771
                     /locus_tag="VI319_07455"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEY6863724.1"
                     /translation="LAVLVAAAAAFYGLHRREDVRAPEAPVPAPAEKAAPATPAPPAD
                     AAPRYEMQPRASEEALPTLENSDAMIGKSISGLVGKKTFERFFIPDMLVRHIVATVDN
                     LPRPTGSTRTVPLHRAPGTLAVTDTGAGLDIDASNTRRYVPYVHIFGSIDARALAERY
                     AATYPLFQRAYEELGYPGRHFNDRLIAAIDDMIAAPELDAPAKVVQSPVIYKFSDPDL
                     ESRSAGQKIMMRLGKENALAVKAKLREIRREIVALTRH"
     gene            complement(800..1918)
                     /locus_tag="VI319_07460"
     CDS             complement(800..1918)
                     /locus_tag="VI319_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY6863725.1"
                     /translation="MRRLLQPFVVVVTALLAAGYAYLALSLASGAGRVALAVPFILIW
                     IVPVFYWSRDRDGGESWLDQAAHQASYLSMALVSFLLVFTFLRDVALVVTSQLPFERV
                     YAMVVQGGVQAVLVASVLAVAIGAVVALRGPGVEEIGVTVPGLHPDLDGFRIVQISDL
                     HVGRTIRRAYVERTVERTRSLAPDLVALTGDMVDGPVDRLAPHVAPLAELARDGRAWF
                     VLGNHDCYSGAASWIAHFRGMGMRVLLNEHAVVRKGAATVLVGGVVDPAYRAARPAIS
                     LEGAPRADFRLLLAHNPKLAPLGARAGFDLQLSGHTHAGQFFPWTLAVRLVHAPHVAG
                     LSREGAMRVYVSAGTGSWGPPVRFGTSPEITLIRLVAA"
     gene            1959..2885
                     /locus_tag="VI319_07465"
     CDS             1959..2885
                     /locus_tag="VI319_07465"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6863726.1"
                     /translation="MTELLETYGLALVFANVLAEQLGLPVPAMPTMMVAAALAAEEHA
                     SLAGLAAATLCAAMTGNAILYAVGERYGQRVMKLLCGISLSPDSCVRQASLRFERWGG
                     WTLVLAKFLPGIGTIAPPLAGVMRFGRGRFLVLSTLGSALWIATALAVGALFHEQIHD
                     ILEALERYGARAAAAVIALVAAYIAFKWWERRRFYRTLRMARITVDELHSLFERKREP
                     LVVDLRGAAERRREGSIPGARVMSFADVEYRLADLPRDRDIVFFCSCPNEASAASAAK
                     LLADLGYARVRPLAGGFDAWVAAGYGVERAAV"
     gene            2895..3761
                     /locus_tag="VI319_07470"
     CDS             2895..3761
                     /locus_tag="VI319_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6863727.1"
                     /translation="MFERFDPRRIETSEAEINLKIGGQGPALLLLHGYPQTHAIWHRV
                     APELANKFTVVAADLRGYGDSSKPHGTPDHATYSKRAMARDQVEVMQQLGFEQFLVVG
                     HDRGARVAHRMALDHAGAVRKLVVLDIVPTRTMYSRVSRDFAKSYYHWFFLLRPEPIP
                     ETMIGANPKFYLEQHMGTRFAGLSAFAPEAMAEYVRCFSDPATIHASCEDYRAAESID
                     LEHDEADLGKKIECPLLALWGEKGTVHRCFKPLEDWRERAADVRGRPLPCGHYIPEEA
                     PADLLAELVKFL"
     gene            3859..4326
                     /locus_tag="VI319_07475"
     CDS             3859..4326
                     /locus_tag="VI319_07475"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY6863728.1"
                     /translation="MSEKPVSDFSLPGTGGKTFRLSAAFSATSSKLVVLYFYPKDNTP
                     GCTAEGQGFRDLHPQFQKAGCSVFGISRDSLKSHESFKAKMGFPFELLADEDETVCKQ
                     FGVIKMKNLYGKKVRGIERSTFVLDRERVIRREWRGVKVPGHVQEVLDFVKSA"
     gene            4373..>5431
                     /locus_tag="VI319_07480"
     CDS             4373..>5431
                     /locus_tag="VI319_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY6863729.1"
                     /translation="MSTRRSHKEPRRAVTKLFVLDTNVLMHDPTSLFRFEEHDVFLPI
                     MTLEELDNNKKGISDASRNARQASRFLDEIVTKHKGPIADGIALARGESDNATGRLFL
                     QTEAINGQLPAGLPSGKADNQIIGVITHLQKKNAERPVILVSKDINMRIKAHALGLEA
                     EDYFNDKVLEDTDLLYTGVTALPADFWEHHGKDVESWKKEGRTYYRVKGPLVGKLLLN
                     EFVHQEGDRPLLAMVREIAGRAAVLETVRDYTHAKNNVWGITARNREQNFALNLLMNP
                     EIDFVTLLGQAGTGKTLLTLAAGLMQTLETKQYSEIIMTRVTVPVGEDIGFLPGTEEE
                     KMTPWIGALEDNLDVLNKD"
BASE COUNT          920 a         1951 c         1812 g          748 t
ORIGIN      
        1 ctcgccgtcc tggtcgccgc ggcggcggcc ttctacggcc ttcatcgccg cgaggacgtg
       61 cgagcgcccg aggcgccggt tcccgcgccg gccgagaagg cggcgcctgc aacgcccgcg
      121 ccgcccgccg acgccgcccc gcgctacgag atgcagccgc gcgcgtccga ggaggcgctg
      181 cccaccctcg agaacagcga cgcgatgatc ggaaagtcga tcagcggcct ggtcgggaag
      241 aagaccttcg agcgcttctt cattcccgac atgctggtgc gccatatcgt cgcgacggtg
      301 gacaacctgc cgcgcccgac cggctcgacg cgcacggtgc cgctgcatcg ggcgcccggg
      361 acgctcgcgg tgaccgacac gggcgccggc ctggacatcg acgcctcgaa cacgaggcgt
      421 tatgtcccct acgtgcacat cttcgggtcg atcgacgcgc gcgcgctcgc cgagcgctat
      481 gcggcgacct atccgctgtt ccagcgcgcc tacgaggagc tcggttatcc cggcaggcat
      541 ttcaacgacc gcctcatcgc ggcgatcgac gacatgatcg ccgcgccgga gctcgacgcg
      601 ccggcgaagg tcgtgcagtc gcccgtgatc tacaagttct ccgaccccga tctcgagtcc
      661 cgctccgccg ggcagaagat catgatgcgc ctgggcaagg agaacgcgct cgccgtcaag
      721 gcgaagctgc gcgagatccg gcgcgagatc gtcgcgctca cgcgccacta gcccgcaccg
      781 gcggcccgag gccccgcgct caggcggcaa cgagccggat cagcgtgatc tcggggctcg
      841 tgccgaagcg caccggcggc ccccacgacc cggtgccggc gctgacgtag acccgcatcg
      901 cgccctcgcg cgagagcccc gcgacgtgcg gcgcgtgcac gaggcgcacg gcgagcgtcc
      961 acgggaagaa ctgccccgcg tgcgtgtggc ccgagagctg gagatcgaag cccgcgcgcg
     1021 cgccgagcgg ggcgagcttc gggttgtgcg cgaggagcag gcggaagtcc gccctcggcg
     1081 cgccctcgag cgagatcgcc ggccgcgcgg cgcgatacgc cgggtcgacc acgccgccga
     1141 cgagcacggt cgccgcgcct ttgcgcacga ccgcgtgctc gttcaagagc acccgcatgc
     1201 ccatgccgcg gaagtgggcg atccacgacg ccgcgccgga atagcagtcg tggttgccga
     1261 gcacgaacca ggcgcgcccg tcgcgggcga gctcggcgag cggcgcgacg tgcggcgcca
     1321 gccggtcgac cgggccgtcg accatgtcgc ccgtgagcgc gacgagatcg ggcgcgagcg
     1381 agcgcgtccg ctcgaccgtg cgctcgacgt aggcgcgccg gatggtgcgc ccgacgtgca
     1441 ggtcggagat ctgcacgatg cggaagccgt cgaggtcggg atgcagtccg ggtaccgtca
     1501 ccccgatctc ctcgacgccc ggaccgcgca acgcgacgac cgcgccgatc gcaacggcga
     1561 gcaccgacgc cacgagcacc gcctgcactc cgccttgcac caccatcgcg tagacccgct
     1621 cgaacgggag ttgcgaggtc acgacgagcg cgacgtcgcg caggaacgtg aagacgagga
     1681 ggaagctcac gagagccatc gaaagatagc tcgcctggtg tgcggcctgg tccagccacg
     1741 actccccgcc gtcgcggtcg cggctccagt agaacaccgg gacgatccag atcaggatga
     1801 aggggacggc gagcgccacg cgtccggccc ccgaggccag gctcagcgca agatacgcgt
     1861 acccggccgc gagcagcgcg gtgaccacca cgacgaacgg ctgcagcagg cgcctcatcg
     1921 cccgcatgct agcgcaaagg ccgatatcat ccgtccgcat gaccgagctc ctcgagacct
     1981 acggcctcgc actcgtgttc gcgaacgtgc tcgccgagca gctcgggctg ccggtgccgg
     2041 cgatgccgac gatgatggtc gcggcggcgc tcgccgccga ggaacacgcg tcgctcgcgg
     2101 gcctggcggc ggcgacgctc tgcgccgcga tgacgggcaa cgcgatcctc tacgcggtcg
     2161 gcgagcgcta cggccagcgc gtgatgaagc tgctgtgcgg gatctcgctc tcgcccgatt
     2221 cctgcgtgcg ccaggcgtcg ctgcgcttcg agcgctgggg cggctggacg ctggtgctcg
     2281 cgaagttcct gcccgggatc ggcacgatcg cgccgccgct cgccggcgtg atgcgcttcg
     2341 gccggggccg cttcctcgtc ctcagcacgc tcggctcggc gctgtggatc gcgaccgcgc
     2401 tcgcggtcgg cgcgctcttc cacgagcaga tccacgacat cctcgaagcg ctcgagcgct
     2461 acggcgcgcg cgcggccgcg gcggtgatcg cgctcgtcgc cgcctacatc gcgttcaagt
     2521 ggtgggagcg gcggcgcttc taccggacgc tgcgcatggc gcgcatcacc gtggacgagt
     2581 tgcactcgct cttcgagcgc aagcgcgagc cgctcgtagt cgacctgcgc ggcgcggccg
     2641 agcgccggcg cgaaggctcg atcccgggcg cccgggtcat gagcttcgcc gacgtcgagt
     2701 accgcctcgc cgacctgccc agggaccgcg acatcgtctt cttctgcagc tgcccgaacg
     2761 aggcctcggc cgcctcggcg gcgaagctcc tcgccgacct cggctacgcg cgcgtgcggc
     2821 cgctcgcggg cgggttcgac gcgtgggtgg cggcgggcta cggcgtggag cgcgccgcgg
     2881 tatagttgcg acccgtgttc gaacgcttcg acccgcgccg catcgagacc tccgaggccg
     2941 agatcaacct gaagatcggc ggccagggtc ccgccctgct cctcctccac ggctacccgc
     3001 agacgcacgc gatctggcac cgcgtcgcgc ccgagctcgc gaataaattc accgtcgtcg
     3061 cggccgacct gcgcggctac ggcgacagct cgaagccgca cggcacgccc gaccacgcga
     3121 cctactccaa gcgcgcgatg gcgcgggacc aggtcgaggt gatgcagcaa ctcggcttcg
     3181 agcaattcct cgtcgtcggc cacgaccggg gcgcgcgcgt cgcgcaccgg atggcgctcg
     3241 atcacgcggg cgcggtgcgc aagctcgtgg tgctcgacat cgtgccgacg cgcacgatgt
     3301 actcgcgcgt atcgcgcgat ttcgcgaagt cgtactacca ctggttcttc ctgctgcgcc
     3361 ccgagccgat cccggagacg atgatcggcg cgaacccgaa gttctacctc gagcagcaca
     3421 tgggcacgcg cttcgccggg ctctcggcgt tcgcacccga ggcgatggcc gagtacgtgc
     3481 gctgcttctc ggacccggcg acgatccacg cgtcgtgcga ggactaccgc gcggccgagt
     3541 cgatcgacct cgagcacgac gaggccgacc tcgggaagaa gatcgagtgc ccgctgctcg
     3601 cgctctgggg cgagaaaggc accgtgcatc ggtgcttcaa gcccctcgag gactggcgcg
     3661 agcgcgccgc cgacgtgcgc gggcggccgc tgccctgcgg ccactacatc cccgaggagg
     3721 cgccggccga tctcctcgcg gagctcgtca agttcctgta acaagcccat gaaaaaataa
     3781 agcttgcatt cggcatcccg gaggcgtaac aatcttcctg acaggggtgc cgacgcactc
     3841 ggagtaccgc cgtattagat gtccgagaaa ccggtcagcg atttcagcct cccgggcacg
     3901 gggggcaaga ccttccgcct ctccgccgca ttttccgcca cgtcgtccaa gctcgtcgtc
     3961 ctgtacttct accccaagga caacacgccc ggctgcaccg ccgagggcca gggcttccgc
     4021 gatctccatc cgcagttcca gaaggccggc tgctcggtct tcgggatctc gcgcgacagc
     4081 ctcaaatccc acgagagctt caaggcgaag atgggcttcc ccttcgagct gctcgccgac
     4141 gaggacgaga ccgtgtgcaa gcagttcggc gtcatcaaga tgaagaacct gtacggcaag
     4201 aaagtgcggg gcatcgagcg cagcaccttc gtgctggaca gggagcgcgt catccgcagg
     4261 gaatggcgcg gagtgaaggt gccgggtcac gtccaggagg tactcgactt tgttaaatcc
     4321 gcttgagcag tccccgcagc ccgcagacga ttccgactca cccaagacga cgatgtccac
     4381 ccgccgatcc cacaaggagc cgcggcgcgc agtcaccaag ctcttcgtcc tcgacaccaa
     4441 cgtcctgatg cacgacccga cgagcctgtt ccggttcgag gagcacgacg tgttcctgcc
     4501 gatcatgacg ctcgaggagc tcgacaacaa caagaagggc atctccgacg cgtcgcgcaa
     4561 cgcgcggcag gcgagccgct tcctcgacga gatcgtgacg aagcacaagg gtccgatcgc
     4621 cgacggcatt gcgctcgcgc gcggcgagtc cgacaacgcc acgggccggc tcttcctcca
     4681 gaccgaggcc atcaacggcc agctgcccgc gggcctgccg agcggcaagg ccgacaacca
     4741 gatcatcggc gtgatcaccc acctgcagaa gaagaacgcc gagcgcccgg tcatcctggt
     4801 gtcgaaagac atcaacatgc gcatcaaggc gcatgcgctc gggctggaag ccgaggacta
     4861 cttcaacgac aaggtgctcg aggacaccga cctcctctac accggcgtca ccgcgctgcc
     4921 cgccgacttc tgggagcacc acggcaagga cgtcgagtcc tggaagaagg aaggccgcac
     4981 ctactaccgc gtcaagggac cgctcgtcgg caagctgctg ctgaacgagt tcgtccacca
     5041 ggagggcgac cggccgctgc tcgccatggt gcgcgagatc gccggccgcg ccgccgtgct
     5101 cgagacggtg cgcgactaca cgcacgcgaa gaacaacgtc tggggcatca ccgcgcgcaa
     5161 ccgggagcag aacttcgcgc tcaacctcct gatgaacccg gagatcgact tcgtcacgct
     5221 gctcgggcag gcgggcaccg gcaagacgct gctcacgctc gcggcgggcc tgatgcagac
     5281 gctcgagacc aagcagtact ccgagatcat catgacgcgc gtcactgtcc ccgtgggcga
     5341 ggacatcggc ttcctccccg gcaccgagga ggagaagatg acgccgtgga ttggcgccct
     5401 cgaggacaac ctcgacgtgc tcaacaagga c
//
LOCUS       DATDMW010000171        10632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_175227, whole genome shotgun sequence.
ACCESSION   DATDMW010000171 DATDMW010000000
VERSION     DATDMW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10632
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175227"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /gene="hcrC"
                     /locus_tag="VI319_07485"
     CDS             complement(<1..447)
                     /gene="hcrC"
                     /locus_tag="VI319_07485"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit gamma"
                     /protein_id="HEY6863730.1"
                     /translation="MKSMKRLVSLLLNGRRREDAVADNLLLLDYLRETAGVTGTKRGC
                     DGGECGACTVLIDGKPRLACLTLAASCEGKTVETVESLAENGRMSALQRAFHEKLGTQ
                     CGFCTPGMIMAAEALLRRNPDPSADDIRAALAGNLCRCTGYVKIIES"
     gene            complement(456..1724)
                     /locus_tag="VI319_07490"
     CDS             complement(456..1724)
                     /locus_tag="VI319_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEY6863731.1"
                     /translation="MPREKLAALQLRRLRQMLRNAYDHVPLHRRRLDAAGCRPEDVRS
                     LRDLAELSFTVKTDLRDHYPFGMFARPREQLVRLHASSGTTGKPTVVGYTAKDIATWA
                     DLMARSMACAGVRPGDVVHNAYGYGLFTGGLGAHYGGERLGATVVPMSGGATERQIVL
                     ITDFRARVLCATPSYALSIAEVAEKQGIDLRASRLEIGLFGAEPWSEAMRKEVEARLG
                     LKAVDIYGLSEILGPGVACECEHQAGLHGWEDHFIFEIIDPESGKALQEGQAGELVIT
                     TLTKEALPMIRYRTRDITRLATERCACGRTHVRIRRITGRTDDMLIIRGVNVYPSQVE
                     AVLVGLPRIAPHYQIVIERRGSLDHVTLEVEAAPGALDSPDAGAPIADSVRHHVKSMV
                     GITVDVMVKQPGEIPRSQGKAVRVRDLRPK"
     gene            complement(1838..2707)
                     /locus_tag="VI319_07495"
     CDS             complement(1838..2707)
                     /locus_tag="VI319_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863732.1"
                     /translation="MIYGSSPWVQRSWDVRAALNFSLGGTGSGMLLVLAFSDVGGDTA
                     LRGAILLALALVAAGLAAVWLEIGRPARAVHVLLNPFTSWMSRESLAAVVLLAVGAGA
                     AATLRVELLIASALAALAFVYCQGRILQNGRGIPAWREPTVTALIVVTALAEGAGALV
                     AVGALARAPMRVALAWCAFAVIARAWAWSAYRRRVTGTVPGPGLAVLDRVGAVLVAVG
                     TLAPLALLLAAALVPPAATVAGVLAGLASLAAGWVFKIALVTRAAYNQGFALPQLPVR
                     GARGTFHRGEARP"
     gene            complement(2704..3360)
                     /locus_tag="VI319_07500"
     CDS             complement(2704..3360)
                     /locus_tag="VI319_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY6863733.1"
                     /translation="MTRWVMIADLRRCVGCQTCTAACRQANATPPGVQWRRVLDMEFG
                     EYPAVKRVFVPVGCMHCDEPPCMQVCPSTATRKRADGVVTIDYDLCIGCSYCAVACPY
                     QARYRTDAPAFAYGEPTAAESEEFDAARIGVATKCTFCVDRIDAGTARGLVPGVDPDA
                     TPACVNSCIAGALHFGDRDDPQSNVSRLLAENRHFRMHESLGTGPGFYYLWDREAEAA
                     "
     gene            complement(3357..6086)
                     /locus_tag="VI319_07505"
     CDS             complement(3357..6086)
                     /locus_tag="VI319_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY6863734.1"
                     /translation="MNARDDAAATRSIPTYCYQCVAGPDLLTVKTVNGVATEVQPNFC
                     AAGEHPGAGRVCVKAYGLVQKTYNPHRLTSPMKRTNPKKGRHEDPGFISISWDEALDT
                     IAERLRALRAFGLLDASGYPKLAASFGGGGTPQSYMGTFPAFLAAWGPVDMGFGSGQG
                     VKCYHSEHLYGELWHRAFTVSPDTPLCDYLISCGANVEASGGVVGVRRHADARARGMK
                     RVQVEPHLSVTGACSAEWVPIRPKTDAAFLFALLHVLLHETPRERLDVAFLEARTSSP
                     YLVGPNGFFLRDPASRKPLVWDARRGAAAPFDAPDVEAALEGVRGTEVDAVEIGADGV
                     VVASGTVEGETSFERLVAHLRPSSPEWAAAVCDVPAATIRRIANEFLDAARVGATIEI
                     EGRMLPYRPVAVSLGKTVNNGWGGYECCWARTMLAALVGALEVPGGTLGTTVRLNRPL
                     SERIKSVKPGADGFMAYPMNPTDPEHWNERPNIRNAYKTLVPLASDGPWSQALGPTHF
                     SWMFLDGTPQGLPEVTLPEVWFVSRTNPAISFWDTESVAAKMARFPFTVAFAYTHDET
                     NHMADILLPDATDLESLQLIRIGGSKYVEQFWRSQGFALRQPAVAPRGESRDFTDVAT
                     ELARRTGILEAYNASINRGAAGVPLKSERWDHSLDTRRAHGRDEIWDAVCRAASAELG
                     DEPRGLDWWKEHGWASRPFPQTDWYLYPALVERGLRFELPYQERLYRIGQELGNRLHD
                     RGMRWWDKQLAEYRPLPEWKDFPGEWTQALVDAGGQPAQFPFWLITARSMQYAWGGNV
                     GMQLIKEVADNVAGHRGVIVNARRAAELGIADGDAVEIATPARSVHGRAVLRHGIRPD
                     TLLLLGQFDHWATPFAKEFGMPSLNALAPMSMALTDATGSGADLLRVALRRAP"
     gene            complement(6083..6493)
                     /locus_tag="VI319_07510"
     CDS             complement(6083..6493)
                     /locus_tag="VI319_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6863735.1"
                     /translation="MKATLKPGLAATRHYPVDRERTIGFMGESARVYATPMLVRDIEV
                     TCRELLLEHLDAGEDSVGTRVEIDHLNPTLLGMEAEITVTVAEVKGRLVTFEIAGSDG
                     VDAIVRGKHSRFVVEVKKTQERLAEKAARAKAAA"
     gene            complement(6577..7278)
                     /locus_tag="VI319_07515"
     CDS             complement(6577..7278)
                     /locus_tag="VI319_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863736.1"
                     /translation="MLRVEGLSSHYGRIPALSGIDLHIERGEMIALVGANGAGKTTLL
                     RALSGVQRVTAGRVLFEGADVTRAPADRRVRLGIVQVPEGRQVFGPLSVEDNLRLGAF
                     VRTRAEAREELERVYALFPALADKRAHAAGTLSGGQQQMLAMGRALMARPRLLLLDEP
                     SMGLAPRLVEEVFARIEALRPEATTVFLVDQNARAALRIADRGYVLETGRIVLSGSGA
                     ELLANERVKAAYLGL"
     gene            complement(7280..8038)
                     /locus_tag="VI319_07520"
     CDS             complement(7280..8038)
                     /locus_tag="VI319_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863737.1"
                     /translation="MLRAESLSKRFGGLNAIADLTFEVERGAVHSVIGPNGAGKTTLL
                     NLLSGVYAPSSGRITFDGRDVTDRAPYQLAARGIARTFQNLQVFFNMSALENVMVGRH
                     LHERCGLAAAMLRPPRVARAERACREESAELMRRVGLGAYLSSRASALPYGALKRLEI
                     ARALAARPRMLLLDEPAAGLNPTETLEIDELIGGIAADGVTVVLVEHNMRLVMGVSDR
                     VLVLDFGQKLAEGTAEAVRGDPRVVEAYLGATEH"
     gene            complement(8041..8988)
                     /locus_tag="VI319_07525"
     CDS             complement(8041..8988)
                     /locus_tag="VI319_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6863738.1"
                     /translation="MLERLAAPRTGGLLALAAFAALLPLALVNNYYYDVAILVGLNAI
                     VCVGLNLLIGYAGQISLGHAGFFGLGAYGSAVLTARYGWPPLAALAAATAGVTVTAFV
                     LGRPILRLRGHYLAMATLGLGVIISIVIATEDRLTGGPDGMAVPPLAVLGAAVQGERV
                     WYWIVAACLLAAVWIALNLVDSPPGRALRALHGTEIGAEVVGIDSARHKLTVFVVSAV
                     FASVAGSLSAHYSGFVTPSKVTFLHSIELVTMVVFGGMASTFGAVVGAAVLTMLPQLL
                     TVLKDYEMLVFGAVLMGTMIFMPRGLVPSLAALAARVRA"
     gene            complement(8997..9872)
                     /locus_tag="VI319_07530"
     CDS             complement(8997..9872)
                     /locus_tag="VI319_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6863739.1"
                     /translation="MSALLVQYLVTGLTVGAVYALVALGFCIIYNASQIINFAQGEFV
                     MIGGMAAVSFSDIGLPLPLAVALAVAAAALVGLALEKLAVEPARHAPVVMLIIITIGA
                     SIFLRGLATLVWDKKLHTLPAFSGDAPIGFFGAVVLPQSLWVLGVTLAIVLLLALFFG
                     RTLLGKAMLATSYNRLAAQLVGIDVRRMLFISFGLSAALGAIAGALIAPIALTSYDIG
                     VMLGLKGFAAAMLGGMGSGTGAVLGGLVLGVAETLGAGYVSSAYKDVIAFAVILAVLF
                     FMPSGLLGVRSSDRV"
     gene            complement(9910..>10632)
                     /locus_tag="VI319_07535"
     CDS             complement(9910..>10632)
                     /locus_tag="VI319_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6863740.1"
                     /translation="PHTDRMACEKIFVDMQSRGMSKIALISGAGGFDKSMRAECLNVA
                     KKHRIDIVADETYGAGDTDMTAQLAKIKSTPGVQAVLNAGFGQGPAIVTKNYRQVDLK
                     LPLYQSHGVASKEFINLAGPAAEGVRLPAAALLVAETLPESDPQKRVVVGYKREYEKR
                     FNSEVSTFGGHAYDGLMLVVEAMKRAGSADKARVRDSLENLRGYMGTAGVVNMGPADH
                     MGLDLTAFRMLEVKNGGWGLVK"
BASE COUNT         1576 a         3783 c         3798 g         1475 t
ORIGIN      
        1 cgactcgatg atcttcacgt aaccggtgca gcggcacagg ttgccggcca gcgcggcgcg
       61 gatatcgtca gccgatggat cgggattgcg ccgcagcagc gcttccgccg ccatgatcat
      121 gccgggcgtg cagaagccgc actgcgtgcc gagcttctcg tggaaggcgc gctgcagcgc
      181 gctcatccgg ccgttctcgg cgagcgactc gaccgtctcg accgtcttgc cttcgcagct
      241 cgccgcgagc gtcaggcacg cgaggcgcgg cttgccgtcg atcagcacgg tgcaggcgcc
      301 gcactcgccg ccgtcgcagc cgcgcttggt cccggtgacg ccggcggtct cgcgcaggta
      361 gtcgagcagc agcaggttgt cggcaaccgc gtcctcgcgg cggcggccgt tcaggaggag
      421 ggagacgaga cgtttcatgc tcttcatcgt tgcgctcatt tcggacgcag atcgcgcacg
      481 cgcacggcct tgccctgcga gcgcggtatc tctccaggtt gcttgaccat gacatcgacg
      541 gtgatgccca ccatcgactt gacgtgatga cgcacgctgt cggcgatcgg tgcgcccgcg
      601 tccggagagt ccagcgcgcc cggcgcggcc tcgacctcga gcgtgacgtg gtcgaggctg
      661 ccgcggcgct cgatcacgat ctggtagtgc ggcgcgatgc gcggcaggcc gaccagcacg
      721 gcctcgacct gcgacgggta gacgttcacg ccgcggatga tgagcatgtc gtcggtgcgt
      781 ccggtgatgc gccggatgcg cacgtgcgtg cgcccgcacg cgcaccgctc ggtggcgagg
      841 cgcgtgatgt cgcgcgtgcg gtagcggatc atcggcagcg cctccttggt gagcgtcgtg
      901 atcacgagct cgcccgcctg gccctcctgc agcgccttgc cgctctcggg gtcgatgatc
      961 tcgaaaatga agtggtcctc ccagccgtgc agcccggcct ggtgctcgca ctcgcacgcg
     1021 actcccgggc ccaggatctc ggacaggccg tagatgtcga cggccttcag gccgaggcgc
     1081 gcctcgactt ccttgcgcat cgcctcgctc cacggctcgg cgccgaacag gccgatctcg
     1141 agccggctcg cgcgcaggtc gatgccctgc ttctccgcga cctccgcgat cgagagcgca
     1201 tacgacggcg tcgcgcacag cactcgcgcc cggaagtcgg tgatcaggac gatctggcgc
     1261 tcggtggcgc cgcccgacat gggcacgacc gtggcgccga ggcgctcgcc gccgtagtgc
     1321 gcgccgagcc ctccggtgaa caggccgtag ccgtaggcgt tgtgcacgac gtcgcccggc
     1381 cgcacgcctg cgcacgccat cgagcgcgcc atcaggtcgg cccacgtggc gatgtccttc
     1441 gccgtgtagc cgacgacggt cggcttgccg gtcgtgcccg acgaggcgtg caggcgcacg
     1501 agctgctcgc gcgggcgcgc gaacatcccg aacggatagt gatcgcgcag gtccgtcttc
     1561 acggtgaagg acagctccgc gagatcgcga aggctgcgga cgtcctcggg cctgcagccg
     1621 gcggcgtcga ggcggcgccg gtgcagcggc acgtggtcgt aggcattccg cagcatctgc
     1681 cgcagccgcc gcagctgcag cgctgcgagc ttctcgcgcg gcatcgtctc ggccgcggcg
     1741 tcgtagcggt agggcggcgc gcgtctcgcg acggcggcgc gtccgcgctt cgccgggcgg
     1801 cgcgcggcgc cgttgcgggt cgcggcagtc atggtgctca tggtcgtgcc tctcctcgat
     1861 ggaacgtccc gcgcgcgccg cgcaccggaa gctggggcag cgcgaatccc tggttatacg
     1921 cggcacgcgt aaccagcgcg atcttgaaga cccagcccgc cgcgagcgac gcgaggccgg
     1981 cgagcacgcc tgccacggtg gcggccggcg gaacgagcgc ggccgcgagc agcagcgcga
     2041 gcggcgcgag cgtgccgacg gcgaccagca cggcgccgac gcgatcgagc accgcgaggc
     2101 cggggccggg cacggtgccc gtgacgcggc ggcgataagc cgaccatgcc caggcgcgcg
     2161 cgatcacggc gaaggcgcac caggcgagtg cgacgcgcat gggcgcccgc gcgagcgcgc
     2221 cgacggcaac gagcgcgccc gcaccttcgg cgagcgcggt gacgacgatc agcgcggtca
     2281 ccgtcggctc gcgccacgcg gggatgccgc ggccgttctg caggatgcgg ccctggcagt
     2341 acacgaaggc gagcgccgcg agcgccgagg cgatgaggag ctctacgcgc agcgtcgcgg
     2401 ccgctcccgc cccgacggcg agcagcacca cggccgcgag cgactcgcgt gacatccacg
     2461 acgtgaacgg attgagcagg acgtggaccg cgcgggcagg ccgcccgatc tcgagccaga
     2521 ccgcggccag gccggccgcg accagcgcga gcgcgaggag aatcgcgccg cgcagcgcag
     2581 tgtcgccgcc gacgtcggag aacgcgagga ccaggagcat gccgctaccc gttccgccga
     2641 gcgagaagtt gagcgccgcg cggacgtccc agctccgctg cacccatggc gacgaaccgt
     2701 agatcatgcc gcctccgctt cccggtccca gaggtagtag aagcccggcc cggtcccgag
     2761 cgactcgtgc atgcggaagt gccggttctc ggcaagcagc cgcgagacgt tgctctgcgg
     2821 gtcgtcgcgg tcgccgaagt gcagggcgcc ggcgatgcac gagttgacgc acgccggcgt
     2881 cgcgtccggg tcgacgccgg gcacgaggcc gcgcgcggtt cccgcgtcga tgcggtcgac
     2941 gcaaaaggtg cacttggtgg cgacgccgat gcgtgcggcg tcgaattctt ccgactccgc
     3001 ggccgtcggt tcgccgtagg cgaacgcggg cgcgtcggtg cggtagcgcg cctggtacgg
     3061 gcaagcgacg gcgcagtacg agcagccgat gcacaggtcg tagtcgatcg tgaccacgcc
     3121 gtcggcgcgc ttgcgcgtcg ccgtggacgg acagacctgc atgcacggcg gctcgtcgca
     3181 gtgcatgcag ccgaccggga cgaacacgcg cttgaccgcg ggatactcgc cgaactccat
     3241 gtcgaggacg cgtcgccatt gcacgccggg cggcgtcgcg ttcgcctggc ggcacgcggc
     3301 cgtgcaggtc tggcagccga cacagcggcg caggtccgcg atcatgaccc agcgcgtcac
     3361 ggcgccctcc tcagcgcgac gcgcagcagg tcggcgcccg agccggtggc gtcggtcagc
     3421 gccatcgaca tcggcgcgag cgcgttcagg ctcggcatgc cgaactcctt cgcgaacggt
     3481 gtcgcccagt ggtcgaactg gccgagcagc agcagggtat cgggacgaat gccgtggcgc
     3541 aggaccgcgc ggccgtgcac gctgcgagcc ggcgtcgcaa tctcgaccgc gtcgccgtcg
     3601 gcgatcccga gctcggcggc gcggcgcgcg ttgacgatca cgccgcggtg cccggcgacg
     3661 ttgtcggcga cttccttgat gagctgcatg ccgacgttgc cgccccacgc gtactgcatg
     3721 ctgcgggcgg tgatcagcca gaacgggaac tgcgcgggct ggccaccggc gtcgacgagc
     3781 gcttgcgtcc attcgccggg gaagtccttc cattccggca gcggccggta ttcggcgagc
     3841 tgcttgtccc accagcgcat gccgcgatcg tgcaggcggt tgccgagctc ctggccgatg
     3901 cggtagaggc gctcctggta cggcagctcg aagcgcaggc cgcgctcgac cagcgcggga
     3961 tagagatacc agtcggtctg cgggaagggc ctgctcgccc agccgtgctc cttccaccag
     4021 tcgaggccgc gcggctcgtc gccgagctcg gcgctcgcgg cgcggcacac ggcgtcccag
     4081 atctcgtcgc ggccgtgcgc gcgccgggtg tcgagcgagt gatcccagcg ctcggacttc
     4141 agcggcacgc cggccgcgcc gcggttgatc gacgcgttgt aggcctcgag gatgcccgtg
     4201 cggcgcgcca gctcggtggc gacgtcggtg aagtcgcgcg actcgccccg cggcgcgacc
     4261 gccggctgcc gcagcgcgaa gccctggctg cgccagaact gctcgacgta cttcgagccg
     4321 ccaatgcgga tcagctgcag gctctcgagg tcggtcgcgt ccggcagcag gatgtccgcc
     4381 atgtggttcg tttcgtcatg cgtatacgcg aacgcgaccg tgaacgggaa gcgcgccatc
     4441 ttcgcggcga cgctctcggt gtcccagaac gagatcgccg ggttggtacg ggagacgaac
     4501 cagacctccg gaagcgtgac ctcgggcagt ccctgcggcg tgccgtcgag gaacatccag
     4561 gagaagtgcg tgggcccgag cgcctggctc cagggcccgt cgctcgcgag cggcaccagc
     4621 gtcttgtagg cattgcggat gttcggccgc tcgttccagt gctcgggatc ggtcgggttc
     4681 atcgggtatg ccatgaaccc gtcagcgccc ggcttgacgc tcttgatgcg ctcggacagc
     4741 gggcggttca ggcgcacggt cgtcccgagc gtgccgcccg gcacctcgag cgcgccgacg
     4801 agcgcggcga gcatggtgcg cgcccagcag cattcgtagc cgccccagcc gttgttcacg
     4861 gtcttgccca ggctcacggc gaccgggcgg tacggcagca tgcggccctc gatctcgatg
     4921 gtcgcgccga cgcgcgccgc gtcgaggaac tcgttcgcga tgcgccggat cgtggccgcc
     4981 ggcacgtcgc acaccgccgc ggcccattcg ggcgagctcg ggcgcaggtg cgcgaccagc
     5041 cgctcgaacg acgtctcgcc ctcgactgtg cccgaggcga cgacgacgcc gtcggcgccg
     5101 atctcgaccg cgtcgacctc ggtcccacgc acgccctcga gcgcggcttc gacgtcgggg
     5161 gcgtcgaacg gagcggccgc gccgcggcgc gcgtcccaca cgagcggttt tcggctcgcc
     5221 ggatcgcgca ggaagaaccc gttcgggccg accagataag gcgacgacgt gcgcgcctcg
     5281 aggaacgcga cgtcgagccg ctcgcgcggc gtctcgtgca gcagcacgtg cagcagggcg
     5341 aacaggaacg cggcgtcggt cttgggacgg atcggcaccc actcggccga gcacgcgccg
     5401 gtgaccgaga ggtgaggctc cacctgcacg cgcttcatgc cgcgcgcgcg cgcgtcggcg
     5461 tgacggcgca cgcccacgac gccgcccgac gcctccacgt tggcgccgca ggagatcagg
     5521 tagtcgcaca gcggcgtgtc gggcgacacg gtgaaggcgc ggtgccagag ctcgccgtag
     5581 aggtgctccg agtgatagca cttcacgccc tgtcccgagc cgaagcccat gtcgaccgga
     5641 ccccaggcgg cgaggaatgc cgggaacgtg cccatgtacg actgcggcgt gccgccgccg
     5701 ccgaagctcg cggccagctt gggatagccc gaggcgtcga gcaggccgaa ggcgcggagc
     5761 gcgcgcagtc gctcggcgat cgtgtcgagc gcttcgtccc acgagatcga aatgaagccc
     5821 gggtcctcgt ggcgtccctt cttcgggttg gtgcgcttca tcggcgaggt cagccggtgc
     5881 gggttgtagg tcttctgcac caggccgtag gccttcacgc acacgcggcc cgcgccggga
     5941 tgctcgcccg ccgcgcagaa gttcggctgc acctcggtcg cgacgccgtt cacggtcttc
     6001 accgtgagca ggtcggggcc cgcgacgcac tggtagcagt acgtcgggat gctgcgcgtt
     6061 gcggcggcgt cgtcgcgggc gttcacgcgg cggccttcgc tctggcggcc ttctcggcga
     6121 gccgctcctg cgtcttcttc acctcgacca cgaaccggga gtgtttcccc cgcacgatgg
     6181 cgtcgacgcc gtcgcttccg gcgatctcga acgtgaccag gcggcccttg acctcggcga
     6241 ccgtcacggt gatctcggct tccatgccga ggagcgtggg gttcaggtga tcgatctcga
     6301 cgcgcgtgcc gaccgagtcc tcacccgcgt ccaggtgctc gagcagcagc tcgcggcagg
     6361 tgacctcgat gtcgcgcacc agcatcggcg tcgcgtagac gcgcgcgctc tcgcccatga
     6421 agccgatcgt ccgctcgcgg tcgacgggat aatgccgggt cgcggcgagc ccgggcttca
     6481 gcgtcgcttt catgctcctc ctcgcctccg gatgttcgtc catcggacca cgggagcccc
     6541 ccgccggact tgatccagat taaacatggt tcactctcac aggccgagat acgcggcctt
     6601 gacgcgctcg ttcgcgagca gctccgcgcc cgagcccgag agcacgatgc ggccggtctc
     6661 gagcacgtag ccgcggtcgg cgatccgcag cgccgcgcgg gcgttctggt ccaccaggaa
     6721 caccgtcgtc gcctcggggc gcagcgcctc gatgcgcgcg aacacctcct cgaccagccg
     6781 cggcgcgagc cccatgctcg gctcgtcgag gagcagcagc cgcggccgtg ccatcagcgc
     6841 gcgtcccatc gcgagcatct gctgctgacc gcccgagagg gtgccggcgg cgtgcgcgcg
     6901 cttgtccgcg agcgccggga acagcgcgta tacgcgctcg agctcctcgc gcgcctcggc
     6961 acgcgtgcgc acgaaggcgc cgaggcgcag gttgtcctcg accgagagcg gcccgaatac
     7021 ctgccggccc tcgggcacct gcacgatgcc gagccggacg cgccggtcgg ccggcgcacg
     7081 cgtgacgtcg gcgccctcga agagcacccg cccggcggtc acgcgctgca cgcccgaaag
     7141 cgcgcgcagc agcgtggtct tgccggcgcc gttggcgccg acgagggcga tcatctcgcc
     7201 gcgctcgatg tgaagatcga tgcccgagag cgccgggatg cgcccgtagt ggctcgagag
     7261 cccctcgacg cgcagcacgt caatgctccg tcgcgcccag gtaggcctcg acgacgcgcg
     7321 gatcgccgcg cacggcctcg gccgtgccct cggcgagctt ctggccgaag tcgaggacga
     7381 ggacgcggtc ggacacgccc atcaccaggc gcatgttgtg ctcgaccagg accacggtga
     7441 ccccgtcggc ggcgatgccg ccgatcagct cgtcgatctc gagcgtttcg gtcgggttga
     7501 gcccggcggc cggctcgtcg agcagcagca tccgcggccg ggcggcgagc gcacgcgcga
     7561 tctcgagccg cttcagcgcg ccgtagggca gggccgacgc ccgcgaggat aagtatgccc
     7621 cgaggcccac tcgccgcatc agctcggcgc tctcctcgcg gcacgcgcgc tcggcgcgcg
     7681 cgacgcgcgg cggccgcagc atcgccgccg cgaggccgca gcgctcgtgc aggtggcgcc
     7741 cgaccatgac gttttcgagc gcgctcatgt tgaagaacac ctgcaggttc tggaacgtgc
     7801 gcgcgatgcc gcgcgcggcg agctggtacg gcgcccggtc cgtgacgtcg cggccgtcga
     7861 acgtgatccg gcccgaggag ggggcgtaga cgccgctcag gagattgagc agcgtggtct
     7921 tcccggcgcc gttgggcccg atcaccgaat gcaccgctcc gcgctcgacc tcgaaagtga
     7981 ggtcggcgat cgcgttcagt ccgccgaagc gcttcgacag cgattcggcg cgcagcaggc
     8041 tcacgcgcgc acccgcgccg cgagcgccgc gaggctgggc acgaggcccc gcggcatgaa
     8101 gatcatggtg cccatcagca ccgcgccgaa cacgagcatc tcgtagtcct tcagcacggt
     8161 cagcagctgc ggcagcatgg tcagcaccgc cgcgccgacc accgcgccga aggtcgacgc
     8221 catgccgccg aacaccacca tggtcacgag ctcgatcgag tgcaggaacg tgaccttcga
     8281 cggcgtgacg aagcccgagt agtgcgccga gaggctgccc gcgaccgaag cgaacaccgc
     8341 cgacacgacg aacaccgtca gcttgtggcg cgcgctgtcg atgccgacca cctcggcgcc
     8401 gatctcggtg ccgtgcagcg cgcgcagcgc gcgcccgggc ggcgaatcga cgaggttgag
     8461 cgcgatccag accgcggcga ggaggcacgc ggcgacgatc cagtaccaga ctcgctcgcc
     8521 ttggaccgcc gcgcccagca ccgcgagcgg cggcaccgcc atgccgtcgg gaccgccggt
     8581 gaggcggtcc tcggtcgcga tcacgatgga gatgatcacg ccgagtccga gcgtcgccat
     8641 ggcgagatag tggccgcgga gcctcaggat cggccggccg agcacgaacg cggtcacggt
     8701 cacgccggcc gtcgcggcgg cgagcgcggc gagcggcggc cagccgtacc gcgcggtgag
     8761 caccgccgag ccgtacgcgc cgaggccgaa gaacccggcg tggcccaggc tgatctggcc
     8821 ggcgtagccg atcagcaggt tgaggccgac gcagacgatc gcgttcagcc cgaccaggat
     8881 cgcgacgtcg taatagtagt tgttgacgag cgcgagcggc agcagcgccg cgaacgcggc
     8941 gagcgcgagc aggcctccgg tgcgaggggc cgcgaggcgc tcgagcacgg tgcgcctcag
     9001 acgcggtcgc tcgaccgcac gccgagcagc ccgctcggca tgaagaacag gaccgcgagg
     9061 atgactgcga acgcgatcac gtccttgtac gccgaggaga cgtagcccgc gcccagcgtc
     9121 tcggcgacac cgagcacgag gccgccgagc accgcgcccg tgccgctgcc catgccgcct
     9181 agcatcgcgg ccgcgaatcc cttcaggccc agcatcacgc cgatgtcgta cgaggtgagc
     9241 gcgatcggcg cgatcagcgc gcccgcaatc gcgcccagcg ccgccgacag gccgaaggag
     9301 atgaatagca tgcggcgcac gtcgatcccg acgagctgcg cggcaagccg gttgtacgag
     9361 gtcgcgagca tcgccttgcc gagaagcgtg cggccgaaga acagcgccag cagcagcacg
     9421 atcgcgagcg tcacgccgag cacccacagg ctctgcggca gcacgaccgc gccgaagaaa
     9481 ccgatcggcg cgtcgccgct gaacgccggc agcgtgtgca gcttcttgtc ccagacgagc
     9541 gtcgcgaggc cgcgcaggaa gatcgacgcg ccgatggtga tgatgatcag catgaccacc
     9601 ggcgcgtggc gcgcgggctc gaccgcgagc ttctcgagcg cgaggcccac gagcgccgcc
     9661 gcggccaccg ccagcgcgac cgcgagcggc aggggcaggc cgatgtccga gaacgacacc
     9721 gcggccatgc cgccgatcat cacgaactcg ccttgcgcga aattgatgat ctggctggcg
     9781 ttgtagatga tgcagaagcc gagagcgacc agcgcgtaga cggcgcccac ggtgagcccc
     9841 gtgacgaggt actggacgag cagcgcgctc atcgtcccga ccgatcgcgc cgccgttccc
     9901 gcggctcggc tacttcacca gcccccagcc cccgttcttc acctcgagca tgcggaacgc
     9961 ggtgaggtcg aggcccatgt ggtcggccgg tcccatgttt accacgccgg cggtgcccat
    10021 gtagccgcgc aggttctcga gcgagtcgcg cacgcgcgcc ttgtccgcgg agcctgcgcg
    10081 cttcatcgcc tcgaccacga gcatgaggcc gtcgtaggcg tggccgccga aggtcgagac
    10141 ctcggagttg aagcgcttct cgtactcgcg cttgtagcca accacgaccc gcttctgcgg
    10201 gtcgctctcg ggcagcgtct cggcgacgag cagcgcggcc gccggcaggc gcacgccctc
    10261 ggccgcgggg ccggcgaggt tgatgaactc cttcgacgcc acgccatgcg actggtacag
    10321 cggcagcttc agatcgacct ggcggtagtt cttggtgacg atcgcgggtc cctggccgaa
    10381 gccggcgttg agcaccgcct gcacgccggg cgtgctcttg atcttggcga gctgggccgt
    10441 catgtcggtg tcgcccgcgc cgtaggtctc gtcggcgacg atgtcgatgc ggtgcttctt
    10501 cgccacgttc aggcactcgg cgcgcatcga cttgtcgaag ccgccggcgc ccgagatgag
    10561 cgcgatcttc gacatgccgc gcgattgcat gtcgacgaag atcttctcgc acgccatgcg
    10621 gtcggtgtgc gg
//
LOCUS       DATDMW010000172         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1294382, whole genome shotgun sequence.
ACCESSION   DATDMW010000172 DATDMW010000000
VERSION     DATDMW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294382"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..553
                     /locus_tag="VI319_07540"
     CDS             95..553
                     /locus_tag="VI319_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY6863741.1"
                     /translation="MQPRLNYRKVAPGVLKTMLDMEAYLGKSSIEHPLLHLIKMRASQ
                     INGCAYCLDMHSKDARAAGETEQRLYALDAWRETPFYTPRERAALAWTESLTLISETH
                     APDDVWSELRAHFSEQEIADLTLAIVAINGWNRLAIGFRVEVGTYQPAKS"
     gene            736..1077
                     /locus_tag="VI319_07545"
     CDS             736..1077
                     /locus_tag="VI319_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863742.1"
                     /translation="MNTLRIIAAIVSALVAGAVCAQTSRSDDKEPLKQGIESVDKNLQ
                     KDPDNKGLQNADKRLRANQQRFDDRRDKRADRREDRTERGERPERAERAERPERAEHV
                     DRVERTERSGR"
     gene            complement(1084..>2505)
                     /locus_tag="VI319_07550"
     CDS             complement(1084..>2505)
                     /locus_tag="VI319_07550"
                     /inference="COORDINATES: protein motif:HMM:NF023689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HEY6863743.1"
                     /translation="MIESGKRVPSEDVLALLSSLFQHAPAWFLDGSPETAPASLAERV
                     GGAAGVPLEPAFLFSKELLQAAIPELLAQTGTTGRQFAHLLIRSHQEMSRNDFPDLER
                     AAERVGERRFPLSVDDLTRLAKHHGLQLRWFERKPVLARDKDRELKSMVRSFFEAPRT
                     VYLNRGLQADPARLKFDLASHLGHKLLHEGDGLKSPHATGGEMGGSPEGGAGLAAGMK
                     PEDVLHAWRDFECSFFAGALLAPKVPFRRFLTRESYRVEAGEKMQLTPAVVMRRMTKV
                     SPYPYWHFFDAYPPGYLRAVYRGNGIPLPWGNMAQVTDPCPQWAVFRMLNEHRGPDRG
                     SQISVLQDGGRSILYCCHSRRVADMAGNPHVLSVGIDLLPALDSHGAASDEIVAGIAD
                     ECRRHGGEARIPKAAVDAIRAAARVLNIAWVEDALAAPARIICPRSTHCPRGEHCDGA
                     RASRAREIGDVREEIVSRARRAR"
BASE COUNT          399 a          891 c          890 g          325 t
ORIGIN      
        1 gggctcgccg ccgcgctcgc ggcgctggcg tgcttcgcct ggagctgaac gaacggaaca
       61 ccgcgaggat ggacatcccg aaaggagctc tctcatgcaa ccccgcctga actaccgcaa
      121 ggtcgccccc ggcgtcctca agaccatgct cgacatggag gcctatctcg gcaagtcgag
      181 catcgagcat ccgctgctgc acctgatcaa gatgcgcgcc tcgcagatca acggctgcgc
      241 ctactgcctc gacatgcact cgaaggacgc gcgcgccgcc ggcgagaccg agcagcggct
      301 gtacgcgctc gacgcctggc gcgagacgcc gttctacacg ccgcgcgagc gcgcggcgct
      361 cgcctggacc gagtcgctga cgctcatctc cgagacgcac gcgcccgacg acgtctggag
      421 cgagctgcgc gcgcatttct ccgagcagga gatcgccgat ctcacgctcg cgatcgtcgc
      481 gatcaacggc tggaaccgcc tcgcgatcgg cttccgggtc gaggtcggca cctaccagcc
      541 cgcgaagagc tgaggcagtc ccgtcgccgc gcactgtctc ccgaaggcga cggaatcgcc
      601 gccggcggcg ctgcgaaacg cgcggagcgt gatctttgct tcacttgcgc cttccggacc
      661 atacggcgcg cgcctacact gttcacgcag gagggtaggc tggcgcgtcc agcaacaacg
      721 aagaaggagc gacgcatgaa cacattgcgc atcatcgcag cgatcgtctc ggcgctggtg
      781 gccggcgccg tatgcgcgca gacctcgagg tcggacgaca aggagccgct gaaacagggc
      841 atcgagagcg tcgacaagaa cctgcagaag gaccccgaca acaaggggct gcagaacgcc
      901 gacaagcgcc tccgtgcgaa ccagcagcgc ttcgacgacc ggcgcgacaa gcgcgccgat
      961 cggcgcgagg accggaccga gcggggcgag cgccccgagc gcgcggaacg cgccgagcgc
     1021 ccggagcgcg ccgagcacgt cgaccgcgtg gagcggacgg agcgctccgg ccgctgattt
     1081 tcgtcagcgc gcgcggcgag cgcggctgac gatctcctcg cgcacgtcgc cgatctcgcg
     1141 cgcccgcgag gcgcgcgcgc cgtcgcagtg ctcgccgcgc gggcagtggg tgctgcgcgg
     1201 gcagatgatg cgcgcgggcg cggcgagcgc gtcctcgacc caggcgatgt tgagcacccg
     1261 ggccgcggcg cggatcgcgt cgacggccgc cttcgggatg cgcgcctcgc cgccgtggcg
     1321 gcggcattcg tcggcgatgc cggcgacgat ctcgtccgag gcggcgccgt gcgagtcgag
     1381 cgccggcagc aggtcgatgc cgaccgagag cacgtgcggg ttgcccgcca tgtcggccac
     1441 gcggcgcgag tggcagcagt agaggatcga ccgcccgccg tcctgcagca ccgagatctg
     1501 cgagccgcgg tccgggccgc gatgctcatt gagcatgcgg aacaccgccc attgcggaca
     1561 cggatcggtg acctgcgcca tgttccccca gggcagcggg atcccgttgc cgcggtagac
     1621 cgcccgcagg tatcccggcg gataggcatc gaagaagtgc cagtacggat acggcgagac
     1681 cttggtcatg cgccgcatga ccaccgccgg cgtgagctgc atcttctcgc ccgcctcgac
     1741 gcggtacgac tcgcgcgtga ggaagcgccg gaacggcacc ttgggcgcga gcagcgcgcc
     1801 cgcgaagaag ctgcattcga agtcgcgcca ggcgtgcagc acgtcctcgg gcttcatgcc
     1861 ggccgcgagc cccgcgccgc cctcggggct gccgcccatc tcgccgccgg tcgcgtgcgg
     1921 cgacttcagg ccgtcgccct cgtgcagcag cttgtgcccg aggtgcgagg cgaggtcgaa
     1981 cttgaggcgc gccggatccg cctgcaggcc gcggttcagg tacacggtgc gcggcgcctc
     2041 gaagaacgag cgcaccatgc tcttcagctc gcggtccttg tcgcgcgcga gcacgggctt
     2101 gcgctcgaac cagcgcagct gcaggccgtg gtgcttcgcg agccgcgtca ggtcgtccac
     2161 ggagagcggg aaccggcgct cgccgacgcg ctcggcggcg cgctcgaggt ccggaaagtc
     2221 gttgcgcgac atctcctggt gcgagcggat gagcaggtgc gcgaactggc gcccggtcgt
     2281 gccggtctgc gccagcagct ccgggatcgc cgcctgcagc agctccttgg agaacaggaa
     2341 cgcgggctcg agcggcacgc cggccgcgcc gccgacgcgc tcggcgagcg aggcgggcgc
     2401 cgtctccggg ctgccgtcca ggaaccacgc cggcgcgtgc tggaacagcg aagacaggag
     2461 cgcgagcacg tcctccgagg gcacgcgctt tccgctctcg atcat
//
LOCUS       DATDMW010000173         4585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2291955, whole genome shotgun sequence.
ACCESSION   DATDMW010000173 DATDMW010000000
VERSION     DATDMW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291955"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /gene="pstC"
                     /locus_tag="VI319_07555"
     CDS             complement(<1..301)
                     /gene="pstC"
                     /locus_tag="VI319_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540924.1"
                     /note="part of the ATP-dependent phosphate uptake system
                     PstABCS responsible for inorganic phosphate (Pi) uptake
                     under Pi starvation conditions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HEY6863744.1"
                     /translation="MSAVANTLEGLAGRAPAAAPASRAIPDARLRRHPRLDLLFRLAT
                     RAFAFLVLALLVAILASLVVGSLPAIGKFGLGFLTDATWNPVTEQFGGLAPIVGTL"
     gene            complement(345..1379)
                     /gene="pstS"
                     /locus_tag="VI319_07560"
     CDS             complement(345..1379)
                     /gene="pstS"
                     /locus_tag="VI319_07560"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY6863745.1"
                     /translation="MRGFRVIAIAGALAAPIAHASDITGAGATFPYPLYAKWAEAYKQ
                     QTGVGLNYQSIGSGGGIKQIRSKTVDFGASDAPLKAEELDKDGLLQFPTVMGGVVPVV
                     NLEGVAPGRMKLTGEALADIYLGRIEKWNDGRIAALNPGMKLPGTPITVVHRADGSGT
                     TFIFTNYLSKVSAEWKVKVGNNASVSWPAPTSTGGKGNEGVASFVQRIKGSIGYVEYA
                     YAKQNRMTFAMLRNRDGEFVAPSDKSFQAAAAGADWKNARGFYEILTDEPGKASWPIT
                     GATFILMHKAQADGAKAKQVLKFFEWAYQNGDRMALELDYVPMPDEVVKLIQSAWKSR
                     MKDASGKPVW"
     gene            1507..1950
                     /gene="sixA"
                     /locus_tag="VI319_07565"
     CDS             1507..1950
                     /gene="sixA"
                     /locus_tag="VI319_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEY6863746.1"
                     /translation="MDLLLWRHADAEPGTRDLERDLTDKGRKQAQRVAEWLRERLPED
                     ARILVSPANRAQQTAGALTKSFATDAALAPGADARDVLAAAGWPDAEGAVLVVGHQPT
                     LGRAAALALTGTSEDWSVKKASVWWLSRRADGKALLRTVISPDLL"
     gene            complement(1961..2521)
                     /gene="msrA"
                     /locus_tag="VI319_07570"
     CDS             complement(1961..2521)
                     /gene="msrA"
                     /locus_tag="VI319_07570"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY6863747.1"
                     /translation="MSTGNQPAPGREIATLAGGCFWCLEAVYDDLKGVESVVSGYMGG
                     ARPNPTYEQVCSGATGHAEVVQVEFDPSKVSYRELLEVFFVIHDPTTPDRQGNDVGTQ
                     YRSAIFYHTPEQQAVARDVMERLGAAKVHDDPIVTQLAPASTFYPAEDYHHEYFRRNP
                     NQPYCAFVVKPKVAKFRKHFLEKTKK"
     gene            2587..3207
                     /gene="msrA"
                     /locus_tag="VI319_07575"
     CDS             2587..3207
                     /gene="msrA"
                     /locus_tag="VI319_07575"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY6863748.1"
                     /translation="MSTVRMLLAALVATAIGAATMPALAQAPQQGLEKATFAGGCFWC
                     MEPPFDKLPGVVSTTSGYTGGTKPNPTYEEVSSGRTGHAESVEVLYDPKQISYEKLLD
                     TFWHNIDPTVRDRQFCDIGTQYRTAIFVHNDEQRRVAERSKAELEKTKPFKQDIVTPI
                     VPASAFYPAEEYHQDFYKKNPIRYKFYRDGCGRDARLKELWGSAPH"
     gene            3213..3671
                     /locus_tag="VI319_07580"
     CDS             3213..3671
                     /locus_tag="VI319_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY6863749.1"
                     /translation="MSDAYTAGARRLTLAGARKIADTAAAAARKAGIAVSIAVVDAGG
                     HLIVLERMDGGRFHTVHSATTKAVSAASNRRATGARGAQGQALDTNHVVGLTLAAGAE
                     RWTAMEGGCPVIVGGECLGGVGVAGGDWKTDEAIAQEAVESIGAAWRDAR"
     gene            3684..>4585
                     /locus_tag="VI319_07585"
     CDS             3684..>4585
                     /locus_tag="VI319_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6863750.1"
                     /translation="MASGPVRVACIGMGWWSDVLADAMVRSGKLRIVSCYTRSEDKRR
                     AFAAKYNCTAAPSYDAVLADRSIEAVVNTTPNNAHLETTRAAARAGKHVFLDKPIANT
                     VSEGREIARACREAGVVLALGYQRRRESHFRWMKAKIEAGEFGRLVNAEANISRDRLG
                     KIDLASWRYTAEGMPGGVMLQIGIHYTDVLAYLMGPIVAVSARSAQLVLPGDNPDVAS
                     LVLEHESGALSTLNASYASASEYYLVNVYGKEASAYYDLHSGLRFLTRGTDRPAPVAC
                     EKRDAFVDELEEFARAVRGEGAPE"
BASE COUNT          697 a         1595 c         1613 g          680 t
ORIGIN      
        1 ccagcgtccc cacgatcggc gcgagcccgc cgaactgctc ggtgaccggg ttccacgtcg
       61 cgtccgtgag gaagccgagg ccgaacttgc cgatcgccgg caggctgccg acgacgagcg
      121 acgcgaggat cgcgaccagc agcgcgagca ccaggaaggc gaacgcgcgc gtcgcgaggc
      181 ggaacagcag gtcgaggcgc gggtgccgcc ggagccgggc atcggggatc gcgcgcgatg
      241 cgggagcggc cgccggagcg cgtccggcga ggccttcgag ggtgttcgcc acggcgctca
      301 ttctatccgc tccccggcgc cgggccccga acgggcacgg cggctcacca gaccggcttc
      361 ccgctcgcgt ccttcatccg gctcttccac gccgactgga tgagcttgac gacctcgtcg
      421 ggcatcggga cgtagtcgag ctcgagcgcc atgcggtcgc cgttctggta ggcccactcg
      481 aagaacttca gcacctgctt cgccttggcg ccgtcggcct gcgccttgtg catgaggatg
      541 aaggtcgcgc ccgtgatcgg ccagctcgcc ttgccgggct cgtcggtgag gatctcgtag
      601 aagccgcgcg cgttcttcca gtcggcgcct gcggccgcgg cctggaagct cttgtcgctc
      661 ggcgcgacga actcgccgtc gcggttcctg agcatcgcga aggtcatccg gttctgcttc
      721 gcgtacgcgt actcgacgta gccgatcgag cccttgatcc gctgcacgaa cgaggcgacg
      781 ccctcgttgc ccttgccgcc ggtgctggtc ggcgccggcc acgagaccga cgcgttgttg
      841 ccgaccttca ccttccactc cgcgctcacc ttcgacaggt agttcgtgaa gatgaacgtc
      901 gtgcccgagc cgtcggcgcg gtgcacgacc gtgatcggcg tgccgggcag cttcatcccc
      961 gggttcagcg ccgcgatccg gccgtcgttc cacttctcga tcctgccgag gtagatgtcg
     1021 gcgagcgcct cgcccgtgag cttcatccgg cccggcgcga cgccctcgag attgacgacc
     1081 gggaccacgc ctcccatcac ggtcggaaac tgcagcaggc cgtccttgtc gagctcttcg
     1141 gccttgagcg gcgcgtcgga ggcgccgaag tcgaccgtct tcgagcggat ctgcttgatg
     1201 cccccgcccg agccgatcga ctggtagttc aggccgacgc ccgtctgctg cttgtaggcc
     1261 tcggcccact tggcgtagag cggatacgga aacgtggcgc ccgcgccggt gatgtcggag
     1321 gcgtgcgcga tcggcgcggc cagcgcgccc gcgatcgcga ttacgcggaa tcctctcatg
     1381 acgttcatga cttcccccgt atcgtgcagt gcaggcgcga tggtaaacgc ccgatatttc
     1441 ggccgcgtta cgatgcgcgc cgggattgtt acgaccccgg cgccgcggcc ggcctaaaat
     1501 caggccatgg acctgcttct ctggcgccac gcggacgccg agcccggaac gcgcgacctg
     1561 gagcgcgacc tcacggacaa gggcaggaag caggcgcagc gcgtcgccga gtggctgcgc
     1621 gaacggctgc ccgaggacgc gcgcatcctc gtgagcccgg cgaaccgcgc gcagcagacc
     1681 gccggggccc tgacgaaatc gttcgcgacc gacgcggcgc tcgcgcccgg cgccgacgcc
     1741 cgcgacgtgc tcgcggcggc cggctggccg gatgcggagg gcgcggtgct cgtcgtcgga
     1801 caccagccga cgctcggacg cgcggccgcg ctcgcgctca ccggtacgag cgaagactgg
     1861 agcgtgaaga aggcgtcggt ctggtggctt tcgcgccgcg cggacgggaa ggcgctgctg
     1921 cggacggtga tatcgcccga cctgctgtag cgcccgaagg ctacttcttc gtcttctcga
     1981 ggaagtgctt gcggaacttc gcgaccttcg gcttcacgac gaaggcgcag tacggctggt
     2041 tgggattgcg gcggaaatat tcgtggtggt agtcctcggc cgggtagaag gtcgaggccg
     2101 gggcgagctg cgtgacgatc ggatcgtcgt ggaccttcgc cgcgccgagc cgctccatca
     2161 cgtcccgcgc aaccgcctgc tgctccggcg tgtggtagaa gatcgccgag cggtattgcg
     2221 tgccgacgtc gttgccctgg cggtcgggcg tcgtcgggtc gtggatcacg aagaacacct
     2281 cgagcagctc acggtacgac actttcgacg ggtcgaactc gacctgcacc acctcagcgt
     2341 gcccggtcgc gccgctgcag acctgctcgt aggtcgggtt ggggcgcgcg ccgcccatgt
     2401 agccggagac gaccgactcc acgcccttca ggtcgtcgta gacggcctcg aggcaccaga
     2461 agcagccgcc ggcgagcgtg gcgatctcgc gtcccggcgc gggttgattt ccggtcgaca
     2521 tgacgcgatt ctaccggcgc ccgccggtcc cgagaaagtt gccggtcggc atggtatgtt
     2581 tgccgcatga gcactgtccg gatgctgctg gcggcgctgg tcgccacagc gatcggggcg
     2641 gcgacgatgc ccgccctcgc gcaagcgccg cagcaaggcc tcgaaaaggc taccttcgcc
     2701 ggcggctgct tctggtgcat ggagccgcct ttcgacaagc tgcccggcgt ggtgtcgacc
     2761 acctcgggct acaccggcgg cacgaagccg aacccgacct acgaggaagt gtcctcgggc
     2821 cgcaccgggc acgccgagtc ggtcgaggtc ctctacgacc cgaagcagat cagctacgag
     2881 aagctgctcg acacgttctg gcacaacatc gatccgaccg tgcgcgaccg ccagttctgc
     2941 gacatcggca cgcagtaccg cacggcgatc ttcgtgcaca acgacgagca gcggcgcgtc
     3001 gccgagcgct cgaaggccga gctggagaag acgaagccgt tcaagcagga catcgtcacg
     3061 ccgatcgtcc cggcctcggc cttctacccg gccgaggagt atcaccagga cttctacaag
     3121 aagaacccga tccgctacaa gttctaccgc gacggctgcg gccgcgacgc gcgcctgaag
     3181 gagttgtggg gcagcgcccc gcactgacgc gcatgagcga cgcctacacc gccggcgcca
     3241 ggcgcctcac gctcgccggc gcgagaaaga tcgccgacac cgccgcggcg gccgcgcgca
     3301 aggccggcat cgcggtctcg atcgccgtgg tcgacgcggg cgggcatttg atcgtgctcg
     3361 agcgcatgga cggcgggcgc ttccacaccg tgcactcggc cacgaccaag gcggtgtccg
     3421 cggcgtcgaa ccggcgcgcg accggcgcgc gcggcgcgca ggggcaggcg ctcgatacca
     3481 atcacgtggt cgggctcacg ctcgccgcgg gggcggagcg ctggaccgcg atggagggcg
     3541 gctgcccggt gatcgtcggc ggcgaatgcc tcggcggcgt cggcgtcgcg ggcggcgact
     3601 ggaagaccga cgaggcgatc gcgcaggagg cggtcgagtc gatcggcgcc gcctggcgcg
     3661 acgcgcggta gccggagcgc gcgttggcgt ccgggccggt ccgcgtcgcc tgcatcggca
     3721 tgggctggtg gtccgacgtg ctcgccgacg cgatggtgcg ctcggggaag ctgcggatcg
     3781 tctcgtgcta cacccgctcc gaagacaagc gccgcgcctt cgccgccaag tacaactgca
     3841 cggcggcgcc gagctacgac gcggtcctcg ccgaccggtc gatcgaggcc gtcgtcaaca
     3901 ccacgccgaa caacgcgcac ctcgagacca cgcgggccgc cgcgcgcgcc ggcaagcacg
     3961 tcttcctcga caagccgatc gccaacacgg tgagcgaggg gcgcgagatc gcgcgcgcgt
     4021 gccgcgaggc gggcgtcgtg ctcgcgctcg gctatcagcg ccgccgcgag agccatttcc
     4081 gctggatgaa agcgaagatc gaggcgggcg aattcgggcg cctcgtgaac gccgaggcca
     4141 acatcagccg tgaccgcctc ggcaagatcg atctcgcgtc gtggcgctac accgcggagg
     4201 gcatgcccgg cggcgtgatg ttgcagatcg gcatccatta caccgacgtg ctcgcgtacc
     4261 tgatggggcc gatcgtcgcg gtgagcgcgc gctcggcgca gctcgtcctg cccggggaca
     4321 acccggacgt cgcgagcctc gtgctcgagc acgagagcgg cgcgctctcg acgctgaacg
     4381 cgagctacgc ctcggcctcc gagtactacc tcgtcaacgt ctacggcaag gaggcgagcg
     4441 cgtactacga cctgcactcg gggctgcgct tcctgacgcg ggggaccgac cggccggcgc
     4501 cggtcgcctg cgaaaagcgc gacgccttcg tcgacgagct cgaggagttc gcgcgcgccg
     4561 tgcgcggcga gggcgcgccg gagat
//
LOCUS       DATDMW010000174        28654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1419964, whole genome shotgun sequence.
ACCESSION   DATDMW010000174 DATDMW010000000
VERSION     DATDMW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 28654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28654
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419964"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VI319_07590"
     CDS             complement(<1..462)
                     /locus_tag="VI319_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY6863751.1"
                     /translation="MFQKILIANRGEIALRIQRACRDLGIRTVVVHSEADRDAKYVKL
                     ADESVCIGPPPSGASYLNIPAIISAAEVTDAEAIHPGYGFLSENADFAERVERSGFVF
                     IGPRADTIRLMGDKINAKAAMLKAGVPCVPGSDGPVPESPEEMIKAGRKIGY"
     gene            complement(472..963)
                     /gene="accB"
                     /locus_tag="VI319_07595"
     CDS             complement(472..963)
                     /gene="accB"
                     /locus_tag="VI319_07595"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY6863752.1"
                     /translation="MDLRKLKKLIDLVEESGVAELEVTEGEEKVRIVKGGMGREPAPP
                     YVVAPALAPYPPQAATASLPAGAAPAPAAVPAPAAAHAAGAEGHVLKAPMVGTFYRAA
                     SPGAKAFAEIGDTVKVGQTVCIIEAMKLMNEIEADKDGVIKAILAENGQPVEYGEPLI
                     VIG"
     gene            complement(1027..1500)
                     /gene="aroQ"
                     /locus_tag="VI319_07600"
     CDS             complement(1027..1500)
                     /gene="aroQ"
                     /locus_tag="VI319_07600"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY6863753.1"
                     /translation="MPKKPARKKAPTRKLRILVVHGPNLNTLGSREPGIYGTQTLDSI
                     DERLQRLAKQAGVDLECFQSNHEGALIDRVHDAKMHGISWIVINPGGLTHTSVALRDA
                     LAAAAIPFVEVHLSNIYARESFRQRSFFSDLAVGTIAGLGSRGYEFALQFAIQSG"
     gene            complement(1578..2216)
                     /locus_tag="VI319_07605"
     CDS             complement(1578..2216)
                     /locus_tag="VI319_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863754.1"
                     /translation="MSRDRPRQQAARLPHQQMRVRIAAVAARLMAEDGIDDYALAKRK
                     AARQLGADDTQSLPGNEEVEAELRAYQSLYQSEEQRERTRELRTKAWQAMQLLAEFRP
                     YLAGAVLKGTAGRYGEIDLQLFTDDSKAVEIALLNRNIPYDVADERRFASGRERAISV
                     LKLVWEGVPLNLSIYASNDERGTLKNSAGGRAIERAGLGAVAQLIQGDSGSG"
     gene            2333..3694
                     /gene="mpl"
                     /locus_tag="VI319_07610"
     CDS             2333..3694
                     /gene="mpl"
                     /locus_tag="VI319_07610"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY6863755.1"
                     /translation="MHLHILGICGTFMGGIAAIAAEAGHTVTGCDANVYPPMSTQLER
                     LGIRLTEGYGADQVAVRPDLWVVGNVVTRGNPLMEALLDRGERLISGPQWLAETVLHG
                     RQVLAVAGTHGKTTVTSMLAWMLEEAGADPGFLIGGVPDNFGISARLGRGPFVIEADE
                     YDTAFFDKRSKFIHYRPRIAILNNLEFDHADIFDDLASIETQFHHFVRTIPSSGTIVV
                     NGNDAALRRVLKRGCWSNVERFGVDDGWHAEEDGTDRFELWRDKARIGGVRWSLAGAH
                     NRANAAAAVAAACSAGFDPAHALASFARFRNVKRRLELRGSAGGVAVYDDFAHHPTAI
                     TATLQALRERVANARILAVLEPRSNTMKLGVMKDELAASLLDADRVFCYSAKLGWDPA
                     AALAPLGAKARCDDDLTSLVEAVAAEARPGDKVLVMSNGGFGGVHEKLLARLGRPSAV
                     SEG"
     gene            3709..4275
                     /locus_tag="VI319_07615"
     CDS             3709..4275
                     /locus_tag="VI319_07615"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEY6863756.1"
                     /translation="MLIYLHGFNSSPRSHKAQVLRQAMEERGLGARFACPALPHWPEE
                     ALAAAERELVARPRDAVTMVGSSLGGFYATCLAERYGVPAVLINPAIEPHVGLRAWLG
                     PQQNPYTGERYELTEEHLHQWQRQYVAPLHLERYLLLVETGDEVLDYRAAVKRYRGAR
                     QVVVQGGDHSLATFPAQIPQILRFAGLA"
     gene            complement(4272..5423)
                     /locus_tag="VI319_07620"
     CDS             complement(4272..5423)
                     /locus_tag="VI319_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY6863757.1"
                     /translation="MPKIASISVATARVPLQHVTSFATRTVSARDYGLVKVRSSEGLQ
                     GIGFCYAGSSGGTLVAHAVEQLLAPLLIGEDSLRVEGLWQRMFQESLLQGRAGSVTRA
                     ISILDCALWDLNARTARLPLYQYLGAAVMDQVPAYASGGYYLDGKTPAKLGAEMAGYV
                     KQGFRAVKMKVGRLSPSEEEARVKAAREAIGPDVELMLDANNAWYDLPTAMQYMRRFE
                     AYDPYWIEEPFFPDDIENHARLAKLTRVPVATGEIGTGRWHFKEILDKGAAAILQTDA
                     LVCGGVSEWKRIAATAASYNITLCPHWFHDLHAHLVAATPNARYVEFFPDDQVLNFRA
                     LIDTQLEQRNGMLVLPRTPGLGFDFDERAVARYAVPAGGTLWTDISSGR"
     gene            5602..7473
                     /locus_tag="VI319_07625"
     CDS             5602..7473
                     /locus_tag="VI319_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HEY6863758.1"
                     /translation="MNVLYEEDGSFKAGTVLADGDTSLQIQAPHGKRAKIKARDVLIR
                     FDEPSAGDVLVQAEQLASDVDLDFLWQCCGSDEFSFIGLARDYYGREPSALQSAAILL
                     KLHSAPMYFYRKGRGRYRAAPPETLRAALAGIEKRKHREQQIAEWSERLAQGELPEEM
                     TPMLDELLYRSDRNRPGTQALERASERTGASPAKLLQRAGALPPSDEYHLNCFLREHF
                     PGGADFPGGLDPVEPADLPPADVNAFSLDDQSTTEIDDAFSLSETADGRLRIGIHIAA
                     PGLGFVPGSPIDVAARARLSTVYMPGGKITMLPPAVIEKFTLAEGRARPALSLYLEVN
                     RDTMAIENRHTRLDVVRIAANLRHQAVGSLDDAFAIGEIPERVPYARELHLLWQVAQA
                     LEASRGKSSAMPDRPDYVFRVDNGRVTIEQRRRGTPLDKLVAELMIAANSAWGKLLDD
                     HGVAAIYRVQSGGRVRMTTSAEAHQALGTSHYAWSTSPLRRYVDLVNQWQLIALLRRE
                     AAPFGRNSDLMLSAVHDFEATYAAYDEFQRRMERYWSLRWLLQEQVRLAPAEVVRETL
                     VKIDGMPLYAKVPSLPQLSPGVRIELEVRDVDVVDMALECRFVRVLEGTAAESAASS"
     gene            7633..8436
                     /locus_tag="VI319_07630"
     CDS             7633..8436
                     /locus_tag="VI319_07630"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY6863759.1"
                     /translation="MLLHGIVLSIRFGFPDPRHPPAAQLDVVLVNSKTRSAPVKPDVQ
                     AQANLDGGGNTDEKRRAKTPLPVLQHNQRGHDLNQARRQVEELEARQRQLLSQLKPSD
                     KPAAQDSRPEETHDPVPRPSGVELASSALMIARMEAQISREIEEYNQRPRRQFVGARA
                     AEARFALYVEHWRQKVEAIGNLNYPDGARGKIYGSLRLTVSINADGSVAGVDLERSSG
                     YKILDAAAERIVTLAAPYAKFPPDIRRDTDVLVITRTWHFAPGDRVFSE"
     gene            8446..9150
                     /gene="mtgA"
                     /locus_tag="VI319_07635"
     CDS             8446..9150
                     /gene="mtgA"
                     /locus_tag="VI319_07635"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY6863760.1"
                     /translation="MRVRARLWKLIAYPLCTLVVAFGLVQLWFLAEVLYWRDHNAAMT
                     RFMELRLEAMRDADRKARLQHRWVEYPRISIHLKRAIIAAEDAKFIDHEGFDWEGIER
                     AIEKNQRRGRYVAGGSTISQQLAKNLFLSGERSLLRKMQEAIITAELETFLTKRRIFE
                     IYLNVVEWADGVFGVEAAAQHYFGISASALNPEQAARLAAMLPRPRYYDRHRDSPYLS
                     QYALTIRGRMEYVQVP"
     gene            9290..10735
                     /gene="mgtE"
                     /locus_tag="VI319_07640"
     CDS             9290..10735
                     /gene="mgtE"
                     /locus_tag="VI319_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY6863761.1"
                     /translation="MSETLSPKKDLEDLQADLREVQELLRRHRAVETAAERSQMPTHE
                     LVESAVHKQNLARLRAKLDSMHPADIAYVLESLPLEERLAVWNLVKAGRDGEILLEAS
                     DAVRETLIRSMDSEELVAAAEQLEADEIADLAPDLPRDVIQDVFQALPIEEREQLRAA
                     MSYPDDAVGALMDFEVVSVREAVTMEAVTRYLRRFDRLPNHTDQLFVVDRDERLKGVL
                     PLSTLIVSDLDRTVEQVMERNMVTLYPDDKAHDAAKAFERYDLVSAAVVDQAGKLVGR
                     VTVDKVVDFIRARTETELLTQAGLREEEDIFSSVWASVRNRWSWLAINLVTAFIASRV
                     IGAFEGSIEKLVALAALMPIVAGIGGNSGNQTITMIVRGIALGQVQNEHGRKLLAKEL
                     GVSLANGLIWGSMLGLIAYWLYHKLSLGLVMTAAMTLNLLLAASAGVLIPLTMWRLGR
                     DPALGSSVLITAITDSGGFFIFLGLATLFLV"
     gene            10759..11181
                     /gene="mscL"
                     /locus_tag="VI319_07645"
     CDS             10759..11181
                     /gene="mscL"
                     /locus_tag="VI319_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY6863762.1"
                     /translation="MAVLREFRDFAVRGNVVDLAVGVVIGAAFGKIVDSLVKDVIMPP
                     IGLALGKTDFTNLFVVLRQGVQPGPYTTLEAAQKAGAVTLNLGMFLNTVVSFVIVAFA
                     IFLLVKLMNRLRREREAAPKEPPEDVRLLREIRDSLKK"
     gene            complement(11192..12481)
                     /gene="hemL"
                     /locus_tag="VI319_07650"
     CDS             complement(11192..12481)
                     /gene="hemL"
                     /locus_tag="VI319_07650"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY6863763.1"
                     /translation="MKSRNQTLFERARAAIPGGVNSPVRAYRGVGGTPPFIARGSGSR
                     LWDVEGKVYIDYVGSWGPLIAGHAHGAVLEAVEAAARDGLSFGAPTEREVQLAELLKE
                     LVPSLELVRLVSSGTEATMTALRLARGYTGRSMVVKFEGCYHGHADSLLVKAGSGALT
                     LGSPDSAGVPPEIAACTLVLPYNDSDALRHAFDRGGKTIAAIIVEPVAGNMNLVVPDR
                     DFLETARRLCSEHGSVLIFDEVMTGFRVALGGAQARFGVRPDLTTLGKVIGGGMPVGA
                     VGGRRDIMERLAPLGPVYQAGTLSGSPVTVAAGLATLALVRTPGFFERLGAETLHLVQ
                     GLTAAARKRGIVFCAQNVGAMFGMYFRAAPPASYGEVMQCDKDAFTRFFHEMLARGIY
                     FAPSAYEAGFVSSAHTRADIDETLAAADAAFAALAGG"
     gene            complement(12478..13068)
                     /gene="thiE"
                     /locus_tag="VI319_07655"
     CDS             complement(12478..13068)
                     /gene="thiE"
                     /locus_tag="VI319_07655"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY6863764.1"
                     /translation="MTPDEPDTALLTAKVERSIRGGARLVQYRNKTAVAALRRRQVDS
                     LLTICRAARVPLIVNDDLDLALALDADGVHLGRDDVDLAVARRSLAPGKLLGASCYDS
                     LELARSATAAGCDYVAFGAAFPTALKPLAPRASLALYAQAKAALRVPIVAIGGITPRN
                     AGSLRQAGVDALAVITALFGAADIETRAREFCAIVS"
     gene            complement(13157..13993)
                     /gene="thiD"
                     /locus_tag="VI319_07660"
     CDS             complement(13157..13993)
                     /gene="thiD"
                     /locus_tag="VI319_07660"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY6863765.1"
                     /translation="MASVPPPPIVLAFAGTDPTGGAGIQADLLTLSSMGCHALSVVTA
                     ITVQDTAGVEDVQAIESDWVSDQARLVLEDMPVAAFKIGLIGSIENVAAIAEVVSDYP
                     DIPLILDPVLASGRGDELATEDMVAAIRELLIPQTTILTPNSREARRLAAEDGEDSEE
                     LDLEACARKILVFGSEFVLITGTHENTPKVINTLYGTEGLVRSDSWERLPGSFHGSGC
                     TLAAAIAATLANGLEIPEAVREAQEYTWQSLNAGFRPGMGQHIPDRFFWARESDDDRS
                     RS"
     gene            14077..14259
                     /locus_tag="VI319_07665"
     CDS             14077..14259
                     /locus_tag="VI319_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEY6863766.1"
                     /translation="MTEKKEFRTWMCLICGFVYDEAAGLPDEGIAPGTHWEDIPINWT
                     CPECGARKEDFEMVEL"
     gene            14527..14886
                     /locus_tag="VI319_07670"
     CDS             14527..14886
                     /locus_tag="VI319_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6863767.1"
                     /translation="MVIDDSNTIRRSAEIFLAQAGCKVILAEDGFDALAKITDHQPDI
                     IFCDIMMPRLDGYQTCALIKKNTRFATTPVIMLSSKDGLFDRARGRMVGSDEYLTKPF
                     TKDSLLKTVGAHAPVKT"
     gene            14900..15265
                     /locus_tag="VI319_07675"
     CDS             14900..15265
                     /locus_tag="VI319_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6863768.1"
                     /translation="MPIKKILIVEDSPTDRQFFMEVLSKQGFQCVTAENGDEAITKSK
                     TEKPDLILMDVVMPGTNGFQATRAITKDEATKHIPVIVCTSKNAETDKIWGMRQGAKD
                     YLVKPIDPKELLAKIAAFK"
     gene            15278..15796
                     /locus_tag="VI319_07680"
     CDS             15278..15796
                     /locus_tag="VI319_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY6863769.1"
                     /translation="MARQISLREFQQSLAQRLREAHAASEQSSRLGVQAGDSFWLLRL
                     DETDEVLPLPEISPVPLTQPWYLGLANIRGVMANVVDFGAFLGDEPTARTPECRLVLV
                     ADRFQSYSGLLISRMMGLRNLQNLEARDAATASERNPWASTLYRDPEGRTWNELNMAA
                     LVAHETFLNVGA"
     gene            16163..18046
                     /locus_tag="VI319_07685"
     CDS             16163..18046
                     /locus_tag="VI319_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY6863770.1"
                     /translation="MLSQRIAKAAQTGLTGNAEAFKQLRASRDQFVTSLKLLTVGGNA
                     SGVSLPATSEDVRPALRELTKEWDKSDQNVSLVLAQEKNLIGLGSAVRSINNNNPALL
                     DLAEQVQALKLQSQAPAREISVAGQLVMLTQRLAKNANAMLGADVIDPEVAFLMGKDT
                     STFRDLLGALTKGSDSLRISATKDPETLEKLADLEKQYQEFQKAVASILGNLQALVNA
                     KNAGRRVVDDSEGLLNAADKLSAAYEDELAQRTLNFIILGGLIVLGIAVVALIVRVYL
                     ADTRRRAEEAERQRGEIERQNKANQDAILRLMNEMGSLAEGDLTVKTTVTEEITGAIA
                     DSVNFTIEELKNVVGRINHAAAQVSIATEAAQKTTTQLLAAAEKQGREIRETSASVLG
                     MARAMNEMSLSATQSADVARQSLAAAQKGTEAVQNSIAGMNEIREQIQETSKRIKRLG
                     ESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFTVVAEEVQRLAERSAEA
                     TKQIGAIVKTIQTDTQDAVSAMEKSTQGVVEGAKLSDAAGQALSEIGDVSRRLAQLIE
                     TISSTTEQQAKSASTVATNMQDILNITQQTTEGTKKTAVSIGQLAQLAQELKGSVSGF
                     KIA"
     gene            18078..23516
                     /locus_tag="VI319_07690"
     CDS             18078..23516
                     /locus_tag="VI319_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY6863771.1"
                     /translation="MTARAEFDVGPLSWVKGEIDLAIQRGLDSLRGFAGDRDVAQIKF
                     SQAQLHQAHGALQIVGLDGITRVSEELEGLLADLEKDAGLRRAETFALAERAFQGILS
                     YLDQLVAGAPNQPLRLFPLYRDLAKARAANREADPTDLYFPDLSFRPPKREGSAAPLR
                     PGAVEQLLREQRAAYQRGFLKFLKGDASGVEEMRGAVLAIDSTQGQTSQRAFWWVAVG
                     FFDALAQKGLSAEHDPKRVCNRIEQQIKRLVEGSSSVAERLMREVLYHVARSRPVTDH
                     VRQVQETFQLEHTLPPQDGVDIVLAPEHPALKSARDLLGQAKDAWNKFASGNPPSLIA
                     FRDAAQAFKDAAAKLGNDDLDNLARETAVLAGWLTSSKDKMSEAIALEVATALLLLEN
                     ALGNLGNLGPEFTQQSQLVCTRLKDCVLGKLLRTTPDIPLLDEMSRRAQERLLMNQVV
                     AEMQTNLRTIEQALDGFFREPAKRDGLAALETPMRQVLGALAMLGEARAQETLSAAGT
                     EIRRFAQPGYVPVQGDFERIAQMLSGLGFYIEALAHGKADFDAAMQPIAPKKPAEAMA
                     SSQPVVTIEAQLDEKKQEAAALYEEWKRKPEDETVKAELQKNLEALQKDAEFVADSRL
                     GTSAEQALKALDASSVLPQEPAIAGAIEQIVPTAASAHSPEAAKMLDASAEQIDAELL
                     GVYLEESEEVLGTIRESLEMCQGQPGNKEALTTIRRGFHTLKGSGRMVGLTRLGEAAW
                     AVEQTMNLWLQEERAVTPDLLKLVQAAHRYFLDNVARLKDGGASSDERELVVMAEKIR
                     AGETLAAPAAAAPVVTAPAAPAIPVEAATARPATADTDRAPRSPTPPRAREPEQFIEI
                     GGIRISATLFTIFSGEARTHLAAIREEHETLKQHGIVTDTLLRAVHTLAGISGTVQLD
                     ALSDLGFALERALQKIASSELSEDEQALVGETIDTVEAMALKAIELEAPAPAPDLIAR
                     LTAVGALAPASSEAVAEAAADAAAEKEIASSFADAVQSQEDDVDAPDALADLGSELDD
                     LQLERRQRRLDDDLDPQLLPIFLEEANELVPSVGAAIRDWKSTPGNPALGNSLQRLLH
                     TLKGSARMAGAMAIGELTHHMEARVEYALSLANLPATLFDELETSYDRMGYLHERIQN
                     PDAALAEPAMEATPAPTAPVVEEQKPVQPAKPAAPESATPLEAVAPQRAAAAEREAIA
                     QRAMLRVRAEVVDRLVNEAGEVSIARSRIEGEMRALKNAMQELTDNVARLRGQLREIE
                     IQAESQMQSRFELAKEAAREFDPLEFDRFTRFQELTRLMAESVNDVSTVHQNLVNTVD
                     ETEKALLAQARLNRDLQQDLMRVRMVPFSSVSERLYRIVRQSAKEVGKRANLDIVGTQ
                     VELDRSVLERITAPFEHLLRNAITHGIETPDKRRAAGKADIGEIRLELTQESNEVQIV
                     FSDDGIGLDIPRIREKAVQMGLLGEHVTLSDAEIADFIFQPGFSTAAEVTQLAGRGVG
                     MDVVRNEIAALGGRIELKFQAGKGTQFTIFLPLTLAVTQAVVVRAGGRIWAIPAVMVE
                     QVLQLREEQLEKAYKNAHAEWQDRRYPFHYLPHLLGSADARAEKTRFSPVLFLRSGSN
                     AISLHVDEMLGSNQEIVVKAIGPQLQRIAGITGATVFGTGEIVLILNPVQLALRDVVA
                     VHAPAVVHEAPKPQAAPTQPTIMVVDDSLTVRKITGRLLERQGYLVVTARDGVEAMEK
                     LQETLPDVMLVDIEMPRMDGFDLTRNVRGDARLKRIPIIMITSRTADKHQNYAREIGV
                     NTFLGKPYQEEQLLAEIHGFLKERRAAA"
     gene            23604..24164
                     /locus_tag="VI319_07695"
     CDS             23604..24164
                     /locus_tag="VI319_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY6863772.1"
                     /translation="MPQVNLTNHFLIAMPNMVDPYFAKSLTYICEHNENGALGLVVNR
                     PIEMTLGTLFERLSLQLASADIGNEPVYFGGPVQTDRGFVLHQPVGQWQSTLSVRDSV
                     GLTTSKDVLEALGQGGGPSKILVTLGYSGWAAGQLENEIRQNAWLTVEAQNAIMFDTP
                     VEERLPAAMQLLGLDFAKLSEEAGHA"
     gene            24161..24607
                     /gene="ruvX"
                     /locus_tag="VI319_07700"
     CDS             24161..24607
                     /gene="ruvX"
                     /locus_tag="VI319_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY6863773.1"
                     /translation="MSATAVAPSKTSGHTILAFDYGEKHVGVAVGDTATRIAHPLARV
                     AAARAFAEIDALVREWNPAELLLGMPLDAQGGEHALSGRVRRFANRLASRYRLPVALI
                     DERFSSTAAEETLRGAGRGGRKDKHLAHALAASTILQGYLDEPARR"
     gene            24588..25094
                     /gene="pyrR"
                     /locus_tag="VI319_07705"
     CDS             24588..25094
                     /gene="pyrR"
                     /locus_tag="VI319_07705"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY6863774.1"
                     /translation="MSLPDAEALCQALVRQMKGHVGEATGMVGIRTGGAWLAERLHRE
                     LGLSTPLGALDISFYRDDFSTSGLRHQVRPSQIPFDVDDRHIVVVDDVLYTGRTVRAA
                     MNELFDYGRPGKIELAVLVDRGERQLPITAQYAGTTLAVPRGKLLRLVRDDSGAFSLV
                     LSDAKPAA"
     gene            25075..26022
                     /locus_tag="VI319_07710"
     CDS             25075..26022
                     /locus_tag="VI319_07710"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY6863775.1"
                     /translation="MRNPQLNERGELTHLLTIEGLPREIVTHILDTAASFVGVTEREV
                     KKVPLLRGKSVFNLFFEPSTRTRTTFEIAAKRLSADVINLAIASSSQTKGETLLDTVD
                     NLSAMHADMFIVRHASSGAAHLIARHVASHVHVINAGDGRHAHPTQGLLDIYTIRHYK
                     QDFTRLSVAIVGDVLHSRVARSLIHALTTLGTPDVRVVGPKTLVPAGVEELGVRVFHD
                     MREGLRDCDVIMMLRLQNERMKGPLLPSAQEFFKSYGLTPQKLALARPDAIVMHPGPM
                     NRGVEIDSAVADGPHSVILPQVTFGIAVRMAVMSILAGN"
     gene            26033..26509
                     /locus_tag="VI319_07715"
     CDS             26033..26509
                     /locus_tag="VI319_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6863776.1"
                     /translation="MTKPPALDPADVESSNASGYPEPYRSRVAGRHKKRLGDAVGLKN
                     FGVNLTTLDPGAESSMRHWHEKQDEFVFVLEGEITLVTNAGRQKLEPGMCAGFPAGRA
                     DGHQLVNETDKPAVYLEIGDRTPGDSATYADPDVDLAVRMVDGKWVFFHRDGRPYR"
     gene            26506..27798
                     /locus_tag="VI319_07720"
     CDS             26506..27798
                     /locus_tag="VI319_07720"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY6863777.1"
                     /translation="MNIHVKNGRLVDPAGRVDSRQDLYIAGGKIAGVGRAPSGFRAER
                     VIDASGLVVCPGLVDLSARLREPGFEDKATLESEMQAAVAGGVTSLSCPPDTDPPLDE
                     PGLVEMLKHRAKSLNLVHVYPLGALTWKLEGQRLTEMAVLRDAGCVAFSHADTPLPDT
                     QVLWRAMQYASTFGFAVWLRPQDGFLARDGVAHDGDVATRLGLPGIPAFAETIALGTI
                     LTLVRATGTRVHLCRISSGEGVALVRAAKKEGLPVTCDVAVHHVHLSEMDLGYFDSHC
                     HVIPPFRSQRDRDALRAGLADGTVDAICSDHAPVDEDAKQLPFAESEPGTTGLELLLP
                     LTLKWAAEARLGLADALAAITARPAAILGIAAGSLAAGADADVCIFDPEEHVRITPEG
                     LHSQGKNTPFIGYELPGRVRYTLVGGQIVFDGGAARAR"
     gene            complement(27795..28085)
                     /locus_tag="VI319_07725"
     CDS             complement(27795..28085)
                     /locus_tag="VI319_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY6863778.1"
                     /translation="MTPWARRVADGWLLSLHVQPGARATQLAGLHGEHLKVRIAASPV
                     EGRANAALEQLIAGALGVPRRCVRVVKGATSRRKTVHVAAPGANPAILEHAP"
     gene            complement(28094..>28654)
                     /locus_tag="VI319_07730"
     CDS             complement(28094..>28654)
                     /locus_tag="VI319_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY6863779.1"
                     /translation="IASLLVQALFGLFVFVVLLRFWMQVLRAPFRNPVGQFVTALSNW
                     IVLPVRRVVPGLLGIDLATVLIAWLAEALTLLVLLSLHGGSLGASPGAGVAVLFGMAF
                     LELIRLSLYLLIGVVLVQVIISWVNPHAPMAGIFDALTRPFYGMFRRFVPPIGGIDLS
                     PLFVLLLAQIMLILLGGLTGMAMRPL"
BASE COUNT         5040 a         9423 c         9588 g         4603 t
ORIGIN      
        1 gtagccgatc ttgcgccccg ccttgatcat ctcctcaggc gactcgggca ccgggccgtc
       61 cgaccccggg acgcatggaa cgccggcttt gagcatcgcc gccttggcgt tgatcttgtc
      121 gcccatcagg cggatcgtgt ccgcgcgcgg accgatgaag acgaagccgc tgcgctcgac
      181 gcgctcggcg aagtcggcgt tctcggaaag gaagccgtat ccagggtgga tggcttccgc
      241 gtcggtcacc tcggcggcgc tgatgatggc cgggatgttc agatagctcg cgcccgaggg
      301 cggcggtccg atgcacaccg actcgtcggc cagcttcacg tacttcgcgt cgcggtcggc
      361 ttcggagtgc acgaccaccg tgcggatgcc gaggtcgcgg caggcacgct ggatgcgcag
      421 ggcgatctcg ccgcggttgg cgatgaggat tttctggaac atgaggcccg gtcagccgat
      481 cacgatgagg ggctcgccgt actccaccgg ctgaccgttt tccgcgagga tggccttgat
      541 gacgccgtcc ttgtcggctt cgatctcgtt catgagcttc atcgcttcga tgatgcatac
      601 ggtctggccc actttcaccg tatccccgat ctcggcaaac gccttcgctc cgggagatgc
      661 ggcgcgataa aaggtcccga ccatcggcgc tttcaagacg tgcccttcgg cgccggcagc
      721 gtgcgcggcg gctggggctg gcacggccgc cggcgcgggc gcggccccgg ctggaaggct
      781 ggcggtcgcc gcctgcggcg gataaggcgc aagcgcgggg gcgacgacgt aaggcggcgc
      841 aggctcgcgg cccatcccgc ctttcacgat cctcactttc tcctcgcctt cggtgacctc
      901 gagctcggcg acgcccgatt cctcgaccag gtcgatcaat ttcttcagct tgcgcaagtc
      961 cattcggcct cctccgatac gcgccgcggc ctccgctgag ccagcgctac tcgggtgttg
     1021 ccggctctag ccggactgaa tagcgaactg cagggcgaac tcgtagcccc gacttccgag
     1081 gccggcgatg gttccaaccg cgaggtcgga gaaaaaagac cgctggcgaa acgactccct
     1141 tgcatagatg ttcgacaagt ggacttccac gaagggaatg gcagcggcgg cgagggcgtc
     1201 ccgcaaagca acgctagtgt gagtcaatcc gcctggattg atgacgatcc aggagattcc
     1261 atgcattttc gcgtcgtgca cgcgatcgat gagagcgcct tcgtgattgc tctggaagca
     1321 ctcgagatcc acgccggcct gcttcgcgag cctctggagc cgttcatcga tgctatcgag
     1381 cgtctgggtg ccgtagattc cgggctccct ggagcccagt gtgttgaggt tcggcccatg
     1441 gacgaccaat atacgcagtt tgcgggtcgg ggccttcttc cgggccggct tcttgggcat
     1501 ccaagaagtt tgccgcaaat ggcaccaagt tgtccacttg atcagcgctt ttgcctgaat
     1561 atcggccccg aagccggcta cccagacccg ctatcgcctt ggatcaactg ggcgacggca
     1621 cccaggccgg ctcgctcgat agcccgcccg cccgcggaat tcttcaatgt gccgcgctcg
     1681 tcgttgctcg catagatgga aaggttcaag ggcacgccct cccagacgag cttgagaacc
     1741 gagatggcgc gctcgcggcc cgatgcgaat cgccgctcgt cggcgacgtc ataggggatg
     1801 ttccggttca ggagcgcgat ctcgaccgcc ttgctgtcgt cggtgaaaag ctgcagatcg
     1861 atctcaccgt agcgcccggc ggtacccttc agcacggcgc cggccaggta tggccggaac
     1921 tccgcgagga gttgcatcgc ctgccatgct ttcgttctga gctcgcgggt tcgctcgcgc
     1981 tgctcctcgc tctgatacag ggactggtag gcacgcagct ccgcctcgac ttcttcgttg
     2041 ccgggaaggg actgtgtgtc gtcagcgccc agctgcctcg cagccttgcg cttcgcgagc
     2101 gcatagtcgt cgatgccgtc ctccgccatg agtcgcgccg cgacagcggc gatgcggaca
     2161 cgcatttgtt gatggggcag gcgcgcagcc tgctgtcgcg ggcggtctcg ggacatcgtg
     2221 gcacgactgt gtccggccat cataccgcag cactcaaccg gcctgaacgg ggccgaacgg
     2281 catccatcgg gtagaatcgc cggtgttcgc cggtttctct gtagccaacc ccatgcatct
     2341 tcacatccta ggaatttgcg gcacgttcat gggcggcatt gccgccattg ccgcggaagc
     2401 cggtcatacc gtgaccggat gcgacgccaa tgtctacccg ccgatgagca cacagctgga
     2461 gcggctcgga atccgtctca ccgaaggcta cggcgcggac caggtggccg tccggccgga
     2521 tctctgggtc gtgggcaatg tggtgacgcg cggcaatcct ttgatggagg cactgcttga
     2581 ccgcggggag cgactcattt cgggtccgca atggctcgcc gagacagtgc tgcacgggcg
     2641 ccaagtgctc gccgtggccg ggactcacgg caaaacgacg gtgacgtcca tgctcgcctg
     2701 gatgctggaa gaggcgggcg ccgatcccgg ttttttgatc ggtggagtcc ccgacaactt
     2761 cgggatctcc gctcgcctcg gccgcggccc attcgtgatc gaggcggacg agtacgacac
     2821 cgctttcttc gacaagcgtt cgaagttcat ccattaccgc cccaggatcg cgattctgaa
     2881 caatctcgag ttcgatcatg ccgacatctt cgatgatctt gcatcgatcg agactcaatt
     2941 tcatcacttt gttcggacga taccgtcttc ggggacgatc gtcgtcaacg ggaacgatgc
     3001 cgctttgagg cgggttctga aacgcggatg ctggagcaac gtggagcgct tcggcgtaga
     3061 cgatggttgg cacgccgagg aggacgggac cgaccgcttc gagttgtggc gcgataaggc
     3121 gcgtatcgga ggagtgcgct ggtccctagc gggcgcgcat aatcgggcga acgctgcagc
     3181 ggctgtcgcg gcggcttgca gcgccggatt cgatccggcg cacgcgctgg catcgttcgc
     3241 tcggtttcgc aacgtcaaaa gaaggctcga gctgcgcggg tccgcgggcg gcgtcgcggt
     3301 ctacgacgac ttcgcgcatc atccgacagc gattacggcg accctgcagg cgctgcgcga
     3361 acgcgtcgcc aatgcgcgga tcctggccgt actggagccg cgctccaata cgatgaagct
     3421 gggcgtgatg aaggacgagc tcgcggcgag ccttctcgat gccgatcgcg tgttctgcta
     3481 cagcgcgaag ctgggctggg accccgccgc agcgctcgcg cctctgggtg cgaaggcgcg
     3541 ctgcgatgac gatctcacca gcttggtcga ggccgtcgcg gccgaggcaa gaccgggaga
     3601 caaggtgctc gtgatgagca acggtggttt cggcggcgtg cacgaaaagt tgctcgcccg
     3661 actcggccgc ccttcagcgg tatccgaagg atagcgccga aacgcgcgat gctgatttat
     3721 ctccacggtt ttaactcttc accgcgctcg cacaaggctc aggtgctgag gcaggcgatg
     3781 gaggagcgcg gcctcggagc tcgcttcgcc tgccccgcgc tgccgcactg gccggaggag
     3841 gcgctcgcgg ccgcggagcg cgagctggtg gcgcgaccgc gcgacgcggt cacaatggtc
     3901 ggaagctcct tgggcgggtt ctacgccacg tgcctcgccg agcgctacgg ggttccggcc
     3961 gtgctgatca atcccgcgat cgagccgcac gtcggcctgc gggcctggct gggtccgcag
     4021 cagaatccat acacaggaga gcgttacgag ctgacggaag agcacttgca tcaatggcag
     4081 cgccagtacg tcgcgccgct acacctggag cgctatctgc tgctcgtgga gacgggcgac
     4141 gaagttctgg attaccgggc tgcggtgaaa cgctatcgcg gcgcgcgcca ggtcgtcgtg
     4201 cagggcggcg accacagcct cgccactttc ccggcgcaga ttcctcagat cctgcggttc
     4261 gcggggctgg cctagcggcc gctgctgatg tcggtccata gggtcccgcc cgcaggaacg
     4321 gcgtagcggg ccacagcgcg ctcgtcgaag tcgaagccca gtcccggcgt gcgcggcagt
     4381 accagcatgc cgttacgctg ttcgagctgc gtgtcgatga gcgcacggaa attgagcacc
     4441 tggtcgtccg ggaagaattc gacgtagcgc gcattcggcg ttgcggccac gaggtgcgca
     4501 tgcaggtcgt gaaaccagtg cgggcatagc gtgatgttgt agctcgcggc agtcgcggcg
     4561 atacgcttcc attcggagac gcccccgcac acgagagcgt cggtctggag gatcgccgcg
     4621 gcacccttgt ccagaatctc cttgaagtgc cagcgcccag tgccgatctc tccggtagcg
     4681 acggggaccc gcgtcagctt cgccagccgg gcgtggtttt cgatgtcgtc cgggaagaac
     4741 ggctcctcga tccagtacgg atcgtaggct tcgaagcgcc tcatgtattg catcgcggtc
     4801 ggaagatcgt accaggcatt gttggcgtcg agcatgagct cgacgtccgg accaatggct
     4861 tcgcgggctg ccttcacgcg cgcctcttcc tcgctgggcg agaggcgacc gaccttcatc
     4921 ttcaccgcgc gaaagccctg cttcacgtag ccggccatct cggcgccgag cttcgccggg
     4981 gtcttgccgt caagatagta gccgccgctc gcgtacgcgg gaacctggtc catgaccgcg
     5041 gctccgaggt actggtacag cggcagtcgc gcggttcgcg cattcaagtc ccacagggcg
     5101 caatcgagga tcgaaatggc gcgcgtgacg ctgcctgcgc gtccctgcag gagcgattcc
     5161 tggaacatgc gctgccatag accttccacc cgcaaactgt cctcgccgat caacagcggc
     5221 gcgaggagct gctcgacggc atgtgcgacc agggtgccgc ccgaggagcc tgcatagcag
     5281 aagccgatcc cttggaggcc ctcgctcgag cgcactttga ccagcccgta gtccctggcg
     5341 gacaccgtgc gcgtggcgaa gctggtgacg tgttggagag ggactcgcgc ggtggcgacg
     5401 gagatcgagg cgatcttggg catggaggcg aggaaccgga tatgagccgg ccgcttcggg
     5461 aagaggacgg cgagcgggcg attatgttga cggtggcgtc gggtgtcaat cgctcgtgcg
     5521 gcggtcgatt gatcctataa tcccgtctga tccatcaagc cggtgctggt ccccccgcgc
     5581 cgttcacccg gaaccgatcc cgtgaacgtt ctctacgagg aagacggctc attcaaggcc
     5641 gggacggtgc tcgctgacgg cgatacctcg ctccagatcc aggcgccgca cgggaaacgc
     5701 gcgaagatca aggccaggga tgtgctgatc cggttcgacg agccctctgc gggcgacgtg
     5761 ctcgttcaag ccgagcagct cgccagcgac gtcgatctgg atttcctttg gcaatgctgc
     5821 ggaagcgatg aattctcgtt catcgggctc gctcgcgatt actacggccg ggagccttcg
     5881 gcgctccagt ccgcggcaat cctgctgaag cttcactcgg ctccgatgta cttctatcgc
     5941 aagggccggg gacgctatcg cgcggctccc ccggagacgc tccgcgccgc cctcgcggga
     6001 atcgagaagc gcaagcatcg ggagcagcag atcgcggagt ggagcgagcg cctggcgcag
     6061 ggtgagctgc ccgaggaaat gacgccgatg ctcgatgagc ttctgtaccg gtccgaccga
     6121 aaccgtcccg ggacacaggc gctcgagcgc gcctccgaac gcaccggcgc gtcgccggcc
     6181 aagcttctac agcgagccgg cgcgcttccc cccagcgatg aatatcacct gaactgcttc
     6241 ctgcgcgagc attttccggg cggcgcggat ttccccggcg gactggatcc cgtcgagccc
     6301 gcggacttgc cgccggccga cgtgaacgca ttcagtctag atgaccagag cacgacggag
     6361 atcgacgacg ccttctcgct gtcggagacg gcggatgggc ggctgcgcat cggcatccat
     6421 atcgcggcgc cgggcctggg gttcgtgccc gggtcgccca tcgatgtggc ggcgcgcgcg
     6481 cggctgtcga ccgtgtacat gcccggcggc aaaatcacga tgctgccgcc ggccgtgatc
     6541 gagaagttca cgctggcgga aggccgcgcg cgtcctgcgc tatcgctcta cctcgaggtg
     6601 aaccgggaca cgatggcgat cgagaaccgg catacccgcc tggatgtcgt tcgcatcgca
     6661 gcgaatctcc ggcatcaagc ggtcgggtcg ctcgatgacg cattcgcgat cggcgaaatt
     6721 ccggagcgcg taccctacgc acgcgagctg catttgctat ggcaggtcgc gcaggccctg
     6781 gaggcgtcgc gcggcaagtc ttcggccatg ccggaccgac ccgattacgt gttccgcgtg
     6841 gacaacggcc gggtcacgat cgagcagcgc cggaggggga cgccgctcga caagctggtg
     6901 gccgagctga tgatcgcggc gaacagcgcg tggggcaagc tgctcgacga ccacggcgtc
     6961 gcggcgatct accgggtgca gtcgggtgga agagtacgca tgacgacctc ggccgaagcc
     7021 caccaggccc tcgggaccag ccattatgca tggtcgacgt ccccgcttcg ccgctatgtc
     7081 gatctggtta accagtggca gctgattgcg ctcctacgtc gggaggccgc cccgttcggg
     7141 cgcaattccg acctcatgct ctcggcggtt cacgacttcg aggcgaccta cgccgcgtac
     7201 gatgagttcc aacgccgcat ggagcgctac tggtcgctgc gctggctgct tcaggagcag
     7261 gttcgcctgg cgccggccga agtggtgcgc gagaccctcg tcaagatcga cggtatgccg
     7321 ttgtacgcga aggttccttc gctaccgcaa ctttcgcccg gggtccggat cgagctcgag
     7381 gtgagggacg tcgacgtcgt cgacatggcc ctcgagtgcc ggttcgtacg cgtcctggag
     7441 ggcaccgccg ccgaatcggc ggcttcgtcc tgagacaaaa ctgtaaatgc agcatagaat
     7501 acgctgatta caaacggctt tgacctgtcg atgtcgagcc tggagcgcgt gttccgagag
     7561 tcgtcctggc tggcgtccct cgccggcctc gaccccggct cgcgtacgct cgcggttgcc
     7621 cttacgcttt cggtgctgct ccacggcatc gtgctgtcga taaggttcgg atttccagat
     7681 ccgcgccatc ctcccgcggc gcagctcgac gtggtgctcg tgaactcgaa aacgcgctcg
     7741 gcgcccgtga aacccgatgt gcaggcccag gcgaatctcg atgggggcgg caacaccgat
     7801 gaaaagcgcc gcgccaagac gcctctgccg gttcttcagc acaaccagcg gggacacgac
     7861 ctcaaccaag ccaggcgtca ggtagaagag ctcgaagcac ggcagcgcca gctgctatcg
     7921 cagctcaagc cgtcggacaa gcccgcggcg caggactcga gacccgagga gacacacgac
     7981 ccggtaccgc gcccgagcgg cgtcgagctc gcgagcagcg cgctgatgat cgcgcgcatg
     8041 gaggcgcaga tctcacgcga gatcgaggaa tacaaccagc gaccgcgccg tcagtttgtc
     8101 ggcgctcgag ccgccgaggc tcgcttcgcg ctctacgtcg agcactggcg ccagaaagtc
     8161 gaagcgatcg gcaacctcaa ctatcccgat ggcgcgcgcg gcaaaatcta cgggagtctg
     8221 cgtctgacgg tctcgatcaa cgccgacggc tcggtcgccg gcgtggactt ggagcggtct
     8281 tcgggctaca agatcctgga cgccgcggcc gagcgcatcg tcacgcttgc cgcgccttac
     8341 gccaagtttc cgcccgacat ccgccgggac accgacgtcc tggtgatcac gcgcacctgg
     8401 catttcgctc ccggggaccg cgtctttagc gaatagccgc gtgccatgag ggtacgggct
     8461 cgtctgtgga agctcatcgc atacccgctg tgtaccctcg tcgtggcgtt cgggctggtc
     8521 cagttatggt ttctcgcgga ggtgctgtac tggcgtgatc acaacgcggc catgacgcgg
     8581 ttcatggagc tgcggctgga agcgatgcgg gacgccgatc ggaaggcaag gctgcagcac
     8641 cgctgggtcg agtatccgcg catctccatc catctcaagc gagcgatcat cgccgcggaa
     8701 gatgccaaat tcatcgatca cgaaggattc gactgggagg gcatcgagcg ggccattgag
     8761 aagaaccagc ggcgcgggcg ttacgtggcg ggcggctcca ccatcagcca acaactcgcc
     8821 aagaaccttt tcctgtccgg tgagcgcagc ctgctgcgca agatgcagga ggcgatcatc
     8881 accgctgaac tcgaaacctt cctcaccaag cgcaggatct tcgagatcta tctcaacgtg
     8941 gtcgagtggg ccgacggggt attcggggtg gaagccgctg cgcagcacta tttcggaatc
     9001 agcgcgtcgg cgttgaatcc cgagcaggcg gcgcgcctcg cagcgatgct gccgcgcccc
     9061 cgctattacg atcgtcaccg ggactcaccg tatctgtcgc aatacgcgct cacgatacgc
     9121 ggccgcatgg agtacgtgca agtaccgtga ccgcgtcggg agcggcgcac gcgctgatgc
     9181 tgagcttccg ggcgccctag cccgctgcta caatcgccgc agccccgcgc gcggcaggcc
     9241 ccggggctgt gagcctccga tccaccacat cgacaagcta tctgggcgaa tgagcgaaac
     9301 cctcagcccg aaaaaggatc tcgaggacct ccaggcggat ctgcgcgaag tgcaggagct
     9361 gctgcgccgc caccgcgcgg tcgagacggc ggccgagcgt tcgcagatgc cgacgcacga
     9421 gctcgtcgag tctgcggtgc acaagcagaa tctcgcgcgg ctgcgcgcca agctcgactc
     9481 gatgcatccg gccgacatcg cgtatgtact tgagtccctg ccgctcgagg agcgtctcgc
     9541 cgtctggaac ctggtcaagg ccgggcgcga cggggagatt ctgctcgagg cgtccgatgc
     9601 cgtgcgcgag acgcttattc gcagcatgga ctccgaggag ctggtcgccg cggccgagca
     9661 gctggaggcc gacgagatcg cggacctcgc acccgacctg ccgcgcgacg tcattcagga
     9721 cgtgttccag gcgctgccga tagaggagcg cgagcagctc cgggcggcaa tgtcctatcc
     9781 ggatgacgcg gtcggcgcgc tcatggattt cgaggtcgtc agcgtgcgcg aggcagtcac
     9841 gatggaagcc gtgacgcgct atcttcgccg cttcgatcgg ctgcccaacc atacggatca
     9901 gctgttcgtc gtcgaccgcg acgagcggtt gaaaggcgtg cttccgcttt cgaccttgat
     9961 cgtgtccgat ctcgatcgca cggtcgagca ggtcatggag cgcaacatgg taacgctcta
    10021 tcccgacgac aaggcgcatg acgcggcgaa ggcgttcgag cgctacgatc tcgtctcggc
    10081 ggccgtggtg gaccaggccg ggaagctcgt cggtcgcgtc accgtcgaca aggtcgtgga
    10141 cttcattcgt gcgcgaacag aaaccgagct tctcacgcag gcgggcctgc gtgaggaaga
    10201 ggacatcttc tcctccgtct gggcgagcgt gcgcaaccgc tggtcctggc tggcaatcaa
    10261 cttggtcacc gccttcatcg cctcgcgcgt gatcggcgcg ttcgagggat cgatagaaaa
    10321 gctggtcgcg ctcgcggcgc tcatgccgat cgtcgcgggt attgggggca attccggcaa
    10381 ccagacgatt acgatgatcg tgcgcggtat cgcgctcggt caggtccaga acgaacacgg
    10441 ccgcaagctt ctggcgaagg agctgggggt ttcgctcgcg aacggactga tctggggcag
    10501 catgctgggg ttgatcgcct actggctgta ccacaagctg tcgctgggcc tggtgatgac
    10561 ggcggcgatg accctcaatc tgctgctcgc cgccagcgcg ggggtcctga tccccttgac
    10621 gatgtggcgg ctcggccgcg acccggcgct cggttccagc gtcctgatca ccgccatcac
    10681 ggatagcggc ggattcttca tttttcttgg cctcgccacg ctgttcctgg tgtagcgggc
    10741 tcactacgga ggtgcgccat ggcggtattg cgggaattcc gggatttcgc agttcggggc
    10801 aacgtcgtgg atctggcggt gggcgtggta atcggcgccg ccttcggaaa gatcgtcgac
    10861 tcgctggtga aggacgtgat catgccgccc atcgggctcg cgctcgggaa gaccgatttc
    10921 accaacctgt tcgtcgttct caggcagggc gtgcagcccg gcccgtatac gacgctggag
    10981 gcggcgcaga aggcgggtgc ggtgacgctc aacctcggaa tgtttctgaa caccgtcgtg
    11041 tcgttcgtca tcgtcgcgtt cgcgatcttc ctgctggtga agctcatgaa cagattgagg
    11101 cgcgagcgcg aagcggcgcc caaggagccg cccgaggacg tgaggctgct gcgcgagatc
    11161 cgcgattcgc tgaagaagta ggacggagag gctagccgcc ggcgagggcc gcgaacgcgg
    11221 cgtccgctgc ggcgagcgtc tcgtcgatgt cggcgcgcgt atgcgcagag gacacgaatc
    11281 ccgcttcgta cgccgaaggt gcgaaataga ttccgcgcgc cagcatctcg tggaagaacc
    11341 gcgtgaacgc gtccttgtcg cactgcatga cttcgccgta gctcgccggg ggggccgctc
    11401 tgaaatacat cccgaacatc gcgcctacgt tttgcgcaca gaacacgatt ccacgcttgc
    11461 gcgccgcggc ggtaaggccc tgcaccaggt gcagcgtctc cgcgcccagg cgctcgaaga
    11521 atccaggcgt gcgcaccagc gccaacgtcg ccaggccggc cgcgacggtg accgggcttc
    11581 ccgagagcgt tcccgcctgg tacaccgggc cgagcggcgc gagccgctcc atgatgtcgc
    11641 ggcgcccgcc gaccgcgccg acgggcatgc cgccaccgat caccttgccg agcgtcgtca
    11701 ggtcgggacg cacaccgaag cgggcttgcg ctcccccgag cgcgacacga aacccggtca
    11761 tcacttcgtc gaagatcagg accgatccgt gctcgctgca aaggcgccgc gcggtctcca
    11821 ggaaatcgcg atccgggacg acgaggttca tgttgccggc gaccggctcg acgatgatcg
    11881 cggcgatcgt cttgccgccg cggtcgaatg cgtgccgcag tgcgtccgag tcgttgtacg
    11941 ggagcaccag cgtgcacgcg gcgatctccg ggggcacgcc cgccgagtcc ggcgatccga
    12001 gggtgagtgc cccggagccg gccttcacga gcaggctgtc ggcgtggccg tgatagcagc
    12061 cctcgaattt cacgaccatg ctgcgccccg tgtagccgcg cgccagccga agcgccgtca
    12121 tggtggcctc ggttcccgag ctcaccaggc gcacgagctc cagcgaaggc accagctcct
    12181 tgagcagctc ggcaagttgc acctcccgct cggtcggcgc tccgaaagac agcccgtccc
    12241 gggccgccgc ctcgaccgcc tcgagcaccg ctccgtgcgc gtgcccggcg atgagcggac
    12301 cccacgagcc gacgtagtcg atgtacacct tcccttccac gtcccagagg cgcgagcccg
    12361 agccacgcgc aatgaacggc ggcgtgccgc cgacgccacg gtacgcacga accggcgaat
    12421 tcacgccgcc gggaatcgct gcacgcgcgc gctcgaacag tgtttgattg cgcgacttca
    12481 tgacacgatt gcgcagaact cgcgcgcgcg cgtctcgatg tccgccgcgc cgaacagcgc
    12541 ggtgatcacg gccaacgcat cgacccccgc ttgcctgagc gatcccgcgt tccgtggtgt
    12601 gatgcccccg atcgcgacga tcggcacgcg cagcgcggcc tttgcctgag cgtaaagagc
    12661 gagcgacgca cgcggcgcca acggcttgag cgcggtcggg aaggctgccc cgaaggcaac
    12721 gtagtcgcag ccggcggcgg tcgcggatcg cgcgagttcg agactgtcgt agcaggaagc
    12781 gcccagcagc tttcccggcg cgagactgcg gcgcgccacg gccagatcga catcgtcccg
    12841 cccgaggtgt acgccatcgg catcgagcgc gagtgccagg tcgaggtcgt cgttgacgat
    12901 aagcggaacg cgcgcagcgc ggcagatggt gagcagcgag tcgacctgtc gcctgcgcag
    12961 cgcggcgacc gcggttttgt tccgatattg cacgagccgg gcgccgccgc gtatggagcg
    13021 ctcgactttc gccgtgagca gcgccgtgtc cggttcgtcg ggcgtcacgg cgtagagacc
    13081 acgcacgcgg gggctggccg cgcggacgtc gctcgggagc agtgaggggg gcgagctcga
    13141 catgcgaggc gctggttcag gaacgcgagc gatcgtcgtc gctttcgcgc gcccagaaga
    13201 aacggtcggg aatgtgctga cccatccccg gccggaatcc ggcgttcaga ctttgccagg
    13261 tgtattcctg cgcttcgcgg acggcctcgg gaatttccag gccattcgcc agggtggcgg
    13321 cgatcgccgc cgcgagcgtg catcccgagc cgtggaaact cccggggagg cgttcccagg
    13381 agtccgagcg cacgagtccc tcggttccgt acagcgtgtt gatcaccttc ggcgtgttct
    13441 cgtgcgtacc cgtgatcagg acgaactcac tgccgaagac gagaatcttg cgcgcgcagg
    13501 cctcgaggtc gagctcttcg ctgtcttcgc cgtcctcggc cgcgagccgg cgcgcttcgc
    13561 ggctgttcgg agtcaggatg gtcgtctgcg ggatgagcag ctcgcggatc gccgcgacca
    13621 tgtcctcggt cgcgagctcg tcgccgcggc ccgaggccaa cacgggatcc agaatgagcg
    13681 gtatgtccgg gtagtcggaa accacttccg cgatcgcggc gacgttctcg atgctgccga
    13741 tcaggccaat cttgaatgcg gcgaccggca tgtcctcgag caccaggcgc gcttggtccg
    13801 agacccaatc cgactcgatc gcctggacgt cctcgacacc ggccgtgtcc tgcacggtga
    13861 tggcggttac gacggacaag gcgtgacagc ccatcgacga gagtgtcaat aaatcggctt
    13921 gtatgccggc gcccccggtc ggatcggtgc ctgcgaaagc gagcacgata ggaggtgggg
    13981 gtacggacgc catggaggtg gaggccccgc gttgctggcg gccggtcagc cagtcggggt
    14041 aagatgggat tggccattgt aacccatccc aacctaatga cggaaaagaa agaattcaga
    14101 acatggatgt gcctgatctg cggcttcgtc tacgatgaag cggccggtct gcctgacgaa
    14161 ggaatcgctc ccggcacgca ttgggaggac atcccgatca actggacttg cccggagtgc
    14221 ggcgctcgga aggaagactt cgaaatggtt gagctgtaac gggcacgggg cgtgcttgat
    14281 cttatccgct tgaccgaaca cacaacatcg gggctacccc ggcgtcataa gccagttttc
    14341 acaggtgttg tgaactaagt aatacaacgc tttgttttga ctgtatagtg tatagcccga
    14401 tccaggctcg tcgtgcaggg ccgacagtcg gggctggagc gagaacaagc tcgcagtaga
    14461 acaagtaatg caaccacggt ggtggaggcc gtgagcgaaa acgccaacct ttccggcatc
    14521 aaggtcatgg tgatcgacga ctcgaacacg attcgtcgca gcgccgagat ttttctcgcc
    14581 caggcgggct gtaaggtcat cctggccgag gacggcttcg acgctctcgc gaaaatcacc
    14641 gaccatcagc cggacatcat cttctgcgac atcatgatgc cgcgcctcga tggctatcag
    14701 acgtgcgcgc tgatcaagaa gaacacgcgc ttcgcgacga ccccggtgat catgctgtcc
    14761 tcaaaagatg gactgttcga ccgtgctcgc ggccgcatgg tcgggtccga cgaatacctg
    14821 accaagccgt tcaccaagga cagcttgctg aagaccgtag gcgcccatgc gccggtaaaa
    14881 acctgaagag gtgaatgcca tgcccatcaa aaaaatcctg atcgtcgaag actcgcccac
    14941 cgaccgccaa ttcttcatgg aagtgctctc gaagcagggc ttccagtgcg ttacggcgga
    15001 aaacggcgac gaggcgatca cgaagtcgaa gaccgagaag cccgatctca ttctgatgga
    15061 cgtggtcatg ccgggaacga acggcttcca ggcgacgcgc gcaatcacca aggacgaggc
    15121 gaccaagcac atcccggtga tcgtctgcac gagcaagaac gccgagaccg acaagatctg
    15181 gggcatgcgc caaggggcga aggactacct ggtcaagccc atcgacccca aagagctgct
    15241 cgcgaagatc gccgctttta agtaatcaag cgatcccatg gcacgccaga tcagtctccg
    15301 cgagttccag caaagcctgg ctcagcggct gcgtgaagcg cacgccgcga gcgagcagtc
    15361 gtcgcggctc ggcgtgcaag cgggtgacag cttctggctc ctgcgactcg acgagacgga
    15421 cgaagtgctg cccctcccgg agatttcgcc tgtgccgctc acgcagccct ggtacctggg
    15481 cctcgcgaac attcgcggcg tgatggccaa cgtggtcgat ttcggggctt tcctcggcga
    15541 cgagccgacg gcgcggacgc cggagtgccg gctggtgctc gtcgcggacc gattccagtc
    15601 ctacagcggc ctgctcatca gccgcatgat gggattgcgg aacctccaga acctcgaggc
    15661 gcgcgacgcc gccacggcgt ccgagcgcaa tccctgggcc agtaccctgt atcgcgatcc
    15721 ggaaggacgc acatggaacg agctcaacat ggcggcgctc gtggcgcacg aaacctttct
    15781 caacgtgggc gcttagcggc ccgcctagct agaagctgat cgggagactg ttatggcatt
    15841 cagactgttg aacatcggaa gcaagaagat tcctgcagcg gcggcagcag ccatgaccgc
    15901 gcccaccgcg aagccctcgc ctgcgccgaa ggccgtcccg aagtccgcgc ccggcgccaa
    15961 gccgacgccg ggcccgacga ccatgtccga aggccccagg cgcgcaggcc tgggtctcgg
    16021 cggcatgcgc gtcggccggc aatacgcgat gctggggacg ttgttcgcgg tggtcttcct
    16081 cgcgtcgatc gtggtcgtcg tactcgacaa ccgcgcggcc acttacggta cggtgtacgt
    16141 gtcgacgtca acccagatgc gcatgttgtc gcagcgcatc gcgaaggcgg cgcagaccgg
    16201 cctgaccggt aacgccgagg cgttcaagca gctgcgcgcc tcgcgcgacc agttcgtgac
    16261 atcgctgaag ctgctcacgg tcggcggtaa cgcgagcggc gtgagcctgc ccgcgacttc
    16321 cgaggacgtg cgtcccgcgc tgagggaact caccaaggag tgggataagt cggaccagaa
    16381 cgtctcgttg gtgctggcgc aggagaagaa cctgatcggt ctcggatccg cggttcgctc
    16441 gatcaacaac aacaacccgg cgctgctcga cctcgccgag caggtccagg ccctgaagct
    16501 tcagagccag gccccggcgc gcgagatttc ggtcgccggt cagctcgtta tgttgacgca
    16561 gcgccttgcg aagaacgcga acgcgatgct gggcgccgac gtgatcgacc cagaagtggc
    16621 gttcctgatg ggtaaggaca ccagtacgtt ccgagacctg ctcggggccc tgaccaaggg
    16681 cagcgactcg ctccggatct ccgcaacgaa ggacccggaa accctcgaaa agctcgccga
    16741 cctcgagaag cagtaccagg agttccaaaa ggccgtggcc tcgatcctcg gtaacctcca
    16801 ggccctggtg aacgcgaaaa acgccggccg tcgcgtcgtg gacgattccg agggactgct
    16861 gaacgccgcc gacaagctgt cggccgcata cgaagacgag ctcgcgcagc ggaccctcaa
    16921 cttcatcatt ctcggcgggc tgatcgtcct cggtatcgcg gtggtcgccc tgatcgtgcg
    16981 tgtgtacctg gccgatacgc gacgccgcgc tgaggaggcg gagcgccagc gtggagagat
    17041 cgaacgccag aacaaggcga accaggacgc gattctgcgg ctgatgaacg agatgggatc
    17101 cctcgcggaa ggcgacctca ccgtgaagac gacggtgacc gaggagatta ccggcgcgat
    17161 cgcggactcg gtcaacttca cgattgaaga gctgaaaaac gtggttggac gaatcaacca
    17221 cgccgctgcg caggtgtcga tcgcgacgga agctgcgcag aagacgacga cgcagcttct
    17281 cgccgctgcc gaaaagcagg gacgagagat tcgcgagacc tcggcatcgg tgctcggcat
    17341 ggcgcgtgcg atgaacgaga tgtcgctgtc cgccacccag tccgcggacg ttgcgcggca
    17401 atcgctggcc gccgcgcaga agggtacgga ggcggtacag aactcgatcg ccggcatgaa
    17461 cgaaatccgc gagcagatcc aggagacgtc gaagcggatc aagcgactcg gcgaatcctc
    17521 gcaggagatc ggcgagatcg tcgagctgat ctctgacatc accgagcaga ccaacgtgct
    17581 cgccttgaac gccgccattc aggccgcgtc ggccggtgaa gcgggacgcg gcttcacggt
    17641 ggtcgcggaa gaagtgcagc gactcgcgga gcgctccgcc gaggcgacga agcagatcgg
    17701 cgcaatcgtg aagacgattc agaccgacac ccaggacgcc gtctccgcta tggagaagag
    17761 cacccagggt gtggtcgaag gagcgaagct gtccgacgcc gccggccagg cgctgtccga
    17821 gatcggcgac gtgtcgcgcc gcctcgcgca gctgatcgag accatttcgt cgacgaccga
    17881 gcagcaggcg aagtccgcga gcacggtggc gaccaacatg caggacattc tgaacatcac
    17941 gcagcagacc accgagggca cgaagaagac cgcggtctcg atcggtcagc tcgcgcagct
    18001 cgcgcaggag ctgaagggtt cggtgtcggg cttcaagatc gcctgagttc agcaacagcg
    18061 aggggacggg gcggggcatg accgcgcgcg cagaattcga cgttgggccg ctttcctggg
    18121 tcaagggcga gatcgacctc gcgatccagc gtggcctgga ctcgctgcgc gggttcgcgg
    18181 gcgatcgcga cgtcgcgcag atcaagttca gccaggcgca gctgcaccag gcgcacggcg
    18241 ccctgcagat cgtcggcctg gacgggatca cgcgcgtatc cgaggagctc gagggactgc
    18301 tcgcggatct cgaaaaggac gctggcctgc gcagggccga gacgttcgcc ctcgccgagc
    18361 gtgcgttcca gggcatcctg tcgtacctcg accagctcgt cgctggcgct ccgaatcagc
    18421 cgctgcgcct tttcccgctg tacagggatc tcgcaaaagc ccgcgcggcc aaccgcgagg
    18481 ccgatccgac cgatctgtat tttccggatc tgtcgtttcg tccgcccaag cgcgaaggct
    18541 cggcggcgcc gctcaggccc ggcgcggtgg aacagctgct gcgcgagcag cgcgcggcat
    18601 atcagcgcgg cttcctcaag ttcctgaagg gtgatgcctc gggcgtcgaa gagatgcgtg
    18661 gcgcggtcct cgcgatcgat tcgacccagg gacagacgtc acaacgggcc ttctggtggg
    18721 tcgccgtggg cttcttcgac gcgcttgcgc agaaggggct gtccgcggag cacgacccga
    18781 agcgcgtctg caaccgcatc gagcagcaga tcaagcgact ggtcgaaggc tcgtccagcg
    18841 tagccgagcg cctgatgcgc gaggtgcttt atcacgttgc ccggtcgagg ccggtgaccg
    18901 atcacgttcg gcaggtgcag gagacttttc agctggagca cacgctgccg ccgcaggatg
    18961 gcgtcgatat cgtgctcgct cccgagcatc ccgccctgaa atccgcgcgc gatttgctgg
    19021 gtcaggcgaa ggatgcttgg aacaagttcg cctccgggaa cccgcccagc ctgatcgcgt
    19081 tccgggatgc cgcgcaggcg ttcaaggatg cggcagcgaa actcggtaac gacgatctcg
    19141 acaatctcgc gcgcgagacg gcagtgctcg cgggctggtt gacgtccagc aaggacaaga
    19201 tgagcgaggc gatcgcgctc gaggtcgcga cggcgctgct gctgctggag aacgcgctcg
    19261 gcaatctggg caacctcggt cccgaattca cgcagcagtc gcagctcgtg tgcacgcgcc
    19321 tcaaggactg cgtgctcggc aagctgctgc gcacgacgcc cgacattccg ctgctcgacg
    19381 agatgtcccg gcgcgcgcag gagcgtctgc tgatgaatca ggtcgtcgcc gagatgcaga
    19441 ccaacctgcg cacgatcgag caggcgctcg acgggttctt ccgcgaaccg gccaagcgcg
    19501 atggtctggc ggcgctcgag acgccgatgc gtcaggtcct cggagcactc gcgatgctgg
    19561 gcgaggcgcg cgcgcaggag acattgagcg ccgcgggcac cgagatcaga cgcttcgcgc
    19621 agccgggtta cgtcccggtg caaggcgact tcgagcggat cgcccagatg ctgtccgggc
    19681 tggggttcta catcgaggcg ctcgcgcacg gcaaggcgga tttcgacgcg gccatgcaac
    19741 cgatcgcccc caagaagccc gccgaggcca tggcctcgtc gcagccggtc gtcaccatcg
    19801 aggcgcaact cgacgagaag aagcaggagg ccgccgccct gtacgaagaa tggaagagga
    19861 agcccgagga cgagacggtg aaagccgagc tccagaagaa cctggaagcg ttgcagaagg
    19921 acgccgagtt cgtcgccgac tcgcgtctcg ggacgtcggc cgagcaggcg ctgaaggcgc
    19981 tcgacgcgag ctcggtgctg ccgcaggagc cggcaatcgc gggcgcgatc gagcagatcg
    20041 tgccgaccgc ggcctcggcg cattcaccgg aagcggccaa gatgctcgac gcaagcgccg
    20101 agcagatcga cgccgagctg ctcggggttt acctcgagga atccgaggag gtcctgggca
    20161 cgatccgcga gagcctcgag atgtgccagg ggcagcccgg caacaaggaa gcgctcacca
    20221 cgatccgccg cggcttccac acgctcaagg gcagtggccg catggtaggc ctcacacgcc
    20281 tcggcgaggc cgcgtgggct gtcgagcaga cgatgaattt gtggctccag gaagagcgcg
    20341 ccgtgacgcc ggacctgctg aagctggtcc aggccgccca ccgctacttc ctggacaatg
    20401 tcgcgcgcct gaaagacggc ggcgcgagca gcgacgagcg cgagctggtc gtcatggcgg
    20461 agaagatccg cgccggagag acgctcgccg cgccggccgc cgcggcaccg gttgtcacgg
    20521 caccggccgc gccggccatt cccgtggaag ccgcaaccgc gcggcctgcc acggctgaca
    20581 cggatcgtgc gccgcgctcg ccgacgccgc cacgcgcgcg ggagcccgag cagttcatcg
    20641 agatcggcgg catccggatt tccgcgacgc tatttacgat cttcagcggc gaggcgcgca
    20701 cgcacctcgc cgcgatacgc gaggagcacg agaccctcaa gcagcacggc atcgtgacgg
    20761 acacgcttct ccgggcggtg catacgctcg cgggtatttc cggcaccgtg cagctcgacg
    20821 cgctgagcga cctgggattc gccctggagc gcgcgttgca gaaaatcgcc tcgagcgagc
    20881 tgtcggagga cgagcaggcg ctcgtcggcg agaccatcga taccgtggaa gcgatggcgc
    20941 tcaaggccat cgagctcgag gccccggcgc ccgcgcccga cttgatcgct cggctgaccg
    21001 ccgtgggcgc gctcgcgccg gcgtcatccg aggccgtggc cgaggcggcc gcggacgcgg
    21061 cggccgagaa ggaaatcgcc tcgtcgttcg cggacgccgt gcaatcgcag gaagacgacg
    21121 ttgacgcccc ggacgcattg gcggacctcg ggtcggagct cgacgacctc cagctcgagc
    21181 gccggcagcg ccgcttggac gacgacctcg acccgcagct gctgccgata ttcctggaag
    21241 aagcgaacga gctggtgcct tcggtgggcg cagcgatccg cgactggaag tccactcccg
    21301 gcaacccggc cctcggcaac tcgctgcagc gcctgctgca cacgctcaag ggcagcgccc
    21361 gtatggcggg cgccatggcg atcggggagc tcacgcacca tatggaggcg cgcgtcgagt
    21421 acgcgctctc gctcgcgaac ctgccggcga cgttgttcga cgagctcgag acctcctacg
    21481 accgcatggg ttatctgcac gagcggatcc agaacccgga cgccgccctc gccgagcccg
    21541 cgatggaggc cacgccggcg cccacagcgc cggtggtgga ggagcagaag ccggtgcaac
    21601 cggccaagcc cgccgctcca gaatccgcga cgcccctcga ggcagtcgct ccacagcgcg
    21661 ctgccgccgc cgagcgcgag gcgatcgcac agcgcgcgat gctgcgcgtg cgcgcagagg
    21721 tcgtcgaccg cctggtcaac gaggcgggcg aggtatcgat cgcccgcagc cggatcgagg
    21781 gcgagatgcg cgccctgaag aacgcgatgc aggaactgac cgacaacgtg gcgcgtctgc
    21841 gcggccagct gcgcgagatc gagatccagg cggaatccca gatgcagtcc cgcttcgagc
    21901 tcgccaagga agccgcgcga gagttcgacc cgctcgagtt cgaccggttc acgcgcttcc
    21961 aggagctgac gcgcctgatg gccgagtcgg ttaacgacgt ctcgaccgtg caccagaacc
    22021 tcgtcaacac cgtcgacgag acggagaagg cgctgcttgc gcaggcgcgg ctcaaccgcg
    22081 acctgcagca ggacctgatg cgggtgcgca tggtgccgtt ctcgagcgta tcggagcgcc
    22141 tctaccgcat cgtacgccag agcgcgaagg aggtcggcaa gcgcgccaac ctcgacatcg
    22201 tggggacgca ggtcgagctg gaccgctcgg tgctcgagcg catcaccgcg cccttcgagc
    22261 acttgctgcg taacgccatc acccacggca tcgagacgcc ggataagcgc cgcgccgccg
    22321 gcaaggccga catcggcgag atccgcctcg agctcaccca ggagagcaac gaggtccaga
    22381 tcgtcttctc ggacgacggc atcggcctcg acattccgcg catccgcgag aaggccgtgc
    22441 agatggggct gctcggggag cacgtgacgc tctcggacgc ggagatcgcc gacttcatct
    22501 tccagccggg cttctcgacc gcagccgagg tgacgcagct cgcgggccgc ggcgtcggca
    22561 tggacgtggt ccggaacgaa atcgcggcgc tgggcggccg catcgagctc aagttccagg
    22621 ccggcaaggg cacgcagttc acgatcttcc tcccgctcac gcttgccgtg acgcaggcgg
    22681 tcgtcgtgcg cgccggcggc cggatttggg cgatccccgc cgtcatggtc gagcaggtgc
    22741 tgcagctgcg cgaggagcag ctcgagaagg cgtacaagaa cgcgcacgcc gaatggcagg
    22801 accgccgcta cccgttccat tacctgccgc acctgctcgg atccgccgac gcgagagcgg
    22861 agaaaacccg attcagcccg gtgctgttcc tgcgcagcgg ctccaacgcg atttcgctgc
    22921 acgtcgacga gatgctgggc agcaaccagg aaatcgtggt gaaggcgatc ggtccgcagc
    22981 tgcagcgtat cgccggcatt accggtgcga cggtgttcgg cacgggcgag atcgtgctga
    23041 tcctgaaccc ggtgcagctc gccctgcgcg acgtcgtggc ggtgcacgcc ccggcggtcg
    23101 tacacgaggc gcccaagccg caggccgcgc ccacgcagcc gaccatcatg gtggtcgacg
    23161 actcgctcac cgtgcgcaag atcaccgggc gcctgctcga gcgccagggc tacctcgtgg
    23221 tcaccgcccg cgacggcgtc gaggcgatgg agaagctgca ggagaccctg cccgatgtca
    23281 tgctcgtgga catcgagatg ccgcgcatgg acggcttcga cctgacccgc aacgtccgcg
    23341 gcgacgcccg cctcaagcgt atcccgatca tcatgatcac ctcgcgcacg gcggacaagc
    23401 accagaacta cgcgcgcgag atcggcgtga acaccttcct gggcaaaccg tatcaggaag
    23461 agcagctgct cgccgagatc cacggtttcc tgaaggagcg ccgggctgcc gcctgagacg
    23521 acctccgggc agtccgaacg gcccgtggtg cgaaattgtg tccgcccgcc tcggcggact
    23581 acaataacga aatcgcccgt cccatgcccc aggtcaacct cacgaatcac ttcctgatcg
    23641 ccatgcccaa catggtcgat ccgtatttcg cgaagagtct gacctacatc tgcgagcaca
    23701 acgagaacgg cgcgctcggc ctcgtcgtga atcgtcccat cgagatgacg ctcggaacgc
    23761 tgttcgagcg gctcagcctg cagctcgcgt cggcggacat cggcaacgag cccgtctact
    23821 tcggcgggcc ggtgcagacc gaccgcggct tcgtgctgca tcagcccgtc ggccaatggc
    23881 aatcgacgct gtcggtgcgc gacagtgtgg ggctgaccac gtccaaggac gtcctcgagg
    23941 cgctcggcca gggcggcggt ccctcgaaga tcctggtgac gctcggatac tccggttggg
    24001 cggccggaca gctcgagaac gagatccggc agaacgcctg gctcaccgtc gaagcgcaaa
    24061 acgccatcat gttcgacact cccgtcgagg agcgcctccc ggccgcgatg cagctgctcg
    24121 gcctggattt cgccaagctg tccgaggagg cggggcacgc gtgagcgcga cggccgtggc
    24181 cccgtccaag acctccggcc acaccattct tgctttcgat tacggcgaaa agcacgtcgg
    24241 cgtcgccgtt ggcgacacgg cgacccgcat cgcgcacccg ctggcccggg tcgccgcagc
    24301 gcgcgcgttc gccgagatcg acgcgctggt gcgcgaatgg aatcccgccg agctgctgct
    24361 cggcatgccg ctcgatgcgc agggcggcga gcacgcgctc tcgggacgcg tacggcgctt
    24421 cgcgaaccgg ctcgcatcgc gctatcgact tccggtggcg ttgatcgacg agcgcttcag
    24481 ctcgaccgcg gccgaggaaa cgctgcgcgg cgccggccgc ggaggccgca aggacaagca
    24541 tctcgcgcac gcgctcgcgg catcgactat cctgcaaggc tatctcgatg agcctgcccg
    24601 acgctgaagc gctgtgccag gcgctcgtgc gccagatgaa gggccacgtg ggcgaggcga
    24661 ccggcatggt cggcatacgc acgggcggcg cgtggctcgc tgaacggctg caccgcgagc
    24721 tcggactctc gactccgctc ggcgcgctcg acatctcctt ctaccgggac gatttcagca
    24781 cctcggggct gcgccaccag gtgcggccct cgcagatccc gttcgacgtc gacgaccggc
    24841 acatcgtcgt cgtcgacgac gtgctctata ccgggcgcac cgtgcgcgcc gcgatgaacg
    24901 agctgttcga ctacggtcgt cctggcaaga tcgagctcgc cgtgctcgtc gaccgcggcg
    24961 agcgacagct gcccatcacc gcgcaatacg ccggtacgac cctcgcggtg ccgcggggga
    25021 agctgctgcg gctggtgcgg gacgactccg gcgccttctc tctcgtgctc tccgatgcga
    25081 aacccgcagc ttaacgaacg cggcgagctc acccacctgc tcaccatcga ggggctgccg
    25141 cgcgagattg tcacgcacat cctcgacacc gcggcctcgt tcgtgggcgt caccgagcgc
    25201 gaggtgaaga aggtcccgct gctgcgcggg aaatcggtgt tcaatctgtt cttcgagccg
    25261 tcgacgcgca cgcgcacgac cttcgagatc gcggcgaagc gcctctcggc cgacgtcatc
    25321 aacctcgcga tcgcgtcgtc ttcgcagacc aaaggcgaga cgctgctcga caccgtggac
    25381 aacctgtcgg cgatgcatgc cgacatgttc atcgtgcggc acgcctcgag cggagcggcg
    25441 cacttgatcg cgcgccacgt cgcctcgcac gtgcacgtga tcaacgccgg cgacggccgt
    25501 cacgcgcatc cgacgcaggg gctgctcgac atctacacca tccggcacta caagcaggac
    25561 ttcacgcgcc tgtcggtcgc gatcgtcggc gacgtgctgc actcgcgcgt cgcgcgctcg
    25621 ctgatccatg cgctcacgac gctcggcacg ccggatgtgc gcgtggtcgg cccgaagacc
    25681 ctggtcccgg ccggcgtgga ggagctcggc gtgcgcgtct tccacgacat gcgcgagggc
    25741 ttgcgcgact gcgacgtcat catgatgctg cggctgcaga acgagcgcat gaaggggccg
    25801 ctgctgccct cggcgcagga gttcttcaag tcctacggac tgacgccgca gaagctcgcg
    25861 ctcgccaggc ccgacgccat cgtcatgcac ccgggcccga tgaaccgcgg cgtggagatc
    25921 gactccgcgg tcgccgacgg cccgcactcg gtcatcctgc cgcaggtgac cttcggcatc
    25981 gccgtgcgca tggcggtgat gagcatcctg gccggaaact gagagaggga cgatgacgaa
    26041 gccgcccgcc ctggatcccg ccgacgtcga atccagcaat gcgagcggct atcccgagcc
    26101 ctaccgcagc cgcgtcgccg gccggcacaa gaagcggctc ggcgacgctg tcgggctgaa
    26161 gaacttcggc gtcaatctca ccacgctcga tccgggcgcc gaatcgtcga tgcgccactg
    26221 gcacgagaag caggacgagt tcgtcttcgt cctcgaaggc gagatcacgc tggtgacgaa
    26281 cgcggggcgc cagaagctcg agcccgggat gtgcgccggc tttcccgccg gccgcgccga
    26341 cggccaccag ctcgtgaacg agaccgacaa gccggccgtg tacctggaga tcggcgaccg
    26401 cacgcccggc gacagcgcca cctacgccga cccggatgtc gatctcgcgg tccgcatggt
    26461 cgacggcaag tgggtcttct ttcaccggga cgggcggccg taccgatgaa catccacgtc
    26521 aagaacggac gcttggtcga ccccgcgggc cgagtcgact ccaggcagga cctgtatatc
    26581 gccggcggaa agatcgcggg cgtgggccgc gcgccctcgg gttttcgcgc cgagcgcgtg
    26641 atcgacgcct cgggcctcgt ggtctgtccg ggcctggtgg atctttccgc gcggctgcgc
    26701 gagcccggct tcgaagacaa ggcgacgctc gagtcggaga tgcaggcggc ggtcgcgggc
    26761 ggcgtgacca gcctctcgtg cccgcccgac accgacccgc cgctcgacga gccggggctg
    26821 gtggagatgc tgaagcaccg cgcgaagagc ctcaacctcg tccatgtcta tccgctcggc
    26881 gcgctgacct ggaagctcga aggccagcgc ctcaccgaga tggcggtcct gcgcgacgcg
    26941 ggctgcgtcg ccttctcgca cgccgacacg ccgcttcccg acacgcaggt cctgtggcgc
    27001 gcgatgcagt acgcgtccac cttcggcttc gcggtgtggc tgcgcccgca ggacggattt
    27061 ctcgcgcgcg acggcgtcgc ccacgacggc gacgtggcga cgcggctcgg cctgcccggc
    27121 atccctgcgt tcgccgagac catcgcgctc ggaacgatcc tgacgctggt gcgggcgacg
    27181 ggcacgcgcg tgcacctgtg ccggatctcg agcggcgaag gcgtcgcgct ggtccgcgcc
    27241 gccaagaaag agggattgcc cgtgacctgc gacgtcgcgg tgcaccacgt gcacctgtcc
    27301 gagatggatc tcggctactt cgactcgcac tgccatgtga ttccgccgtt ccgctcgcag
    27361 cgggaccgcg acgccctgcg cgccgggctc gccgacggca cggtcgacgc catctgctcg
    27421 gaccacgcgc cggtcgacga ggacgcgaag cagctcccgt tcgcggagtc ggagccgggc
    27481 acgaccggtc tggagctgct gctgccgctc acgctcaagt gggcggcgga ggcgcgcctc
    27541 ggcctggccg acgcgctcgc cgcgatcacg gcacggccgg ctgcgatcct gggcatcgcc
    27601 gcgggcagct tggctgcggg cgccgatgcg gacgtctgca tcttcgatcc cgaggagcac
    27661 gtacgcatca cccccgaggg cctgcacagc cagggaaaga acacgccctt catcggctac
    27721 gagctgcccg gacgcgtgcg ctacacgctg gtcggcgggc agatcgtgtt cgacggcggc
    27781 gcggcgcgcg cgcgctaagg agcgtgctcg aggatcgccg ggttcgcgcc gggagcggcg
    27841 acgtgcacgg tcttgcgccg cgatgtcgcg cccttcacga cgcggacgca gcgcctaggg
    27901 accccgagcg cgccggcgat gagctgctcg agcgccgcgt tcgcgcggcc ctcgaccggc
    27961 gacgctgcga tccgcacctt cagatgctcg ccgtgcaggc cggcgagctg cgtcgccctc
    28021 gcgcccggct ggacatggag cgatagcagc cagccgtcgg cgacccgccg ggcccacggt
    28081 gtcatggtcg cgcctacagc ggtcgcatcg ccattccggt gaggccgccg agcaggatca
    28141 gcatgatctg cgcgagcagc aggacgaaaa gcggcgagag atcgatgccc ccgatgggcg
    28201 gcacgaagcg cctgaacatc ccgtaaaagg gtcgcgtcag cgcgtcgaag atgccggcca
    28261 tgggggcgtg tggattcacc cagctgatga tcacctgcac gagcacgact ccgatcagca
    28321 ggtagagcga caggcggatc agctcgagaa acgccatgcc gaagagcact gcgacgcccg
    28381 cgccgggcga ggcgccgagc gatccgccgt gcagcgagag gagaacgagc agcgtcagcg
    28441 cctccgcgag ccaggcgatc agaacggttg cgaggtcgat gccgagcagc cctgggacga
    28501 ctcgacgcac cggcagcacg atccagttcg agagcgcggt gacgaactgc ccgacgggat
    28561 tgcggaaggg cgcccgcagg acctgcatcc agaaccgaag cagcacgacg aacacgaaca
    28621 ggccgaacag cgcctggacg aggaggctcg cgat
//
LOCUS       DATDMW010000175         2550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_797984, whole genome shotgun sequence.
ACCESSION   DATDMW010000175 DATDMW010000000
VERSION     DATDMW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797984"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VI319_07735"
     CDS             <1..228
                     /locus_tag="VI319_07735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6863780.1"
                     /translation="LVDPKRELDEIFSAGERAGMLIYRDDDELVRLARSLPGRATELR
                     AAAREAAPSFHRNHSYLARVAEMMRILAARA"
     gene            complement(256..332)
                     /locus_tag="VI319_07740"
     tRNA            complement(256..332)
                     /locus_tag="VI319_07740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(296..298),aa:Pro,seq:cgg)
     gene            complement(374..1351)
                     /locus_tag="VI319_07745"
     CDS             complement(374..1351)
                     /locus_tag="VI319_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY6863781.1"
                     /translation="MSFVSMLAPVADDLRRVDELIGARLESDVPLVREVAQYIVASGG
                     KRLRPALLLLACGAAGAAGKSAEARFALAAVIEFIHTATLLHDDVVDESRLRRGQPTA
                     NAAFGNAAAVLVGDFLYSRAFQMMVEVDDMRVMRVLADATNTIAAGEVLQLMGSHDPE
                     VDEARYLEVIRRKTARLFEAAARLGGVLARSPAALENGLAAYGAHVGTAFQLIDDVLD
                     YSGDAATIGKSLGDDLAEGKPTLPLIHVLRNGTPAQAGLVRRAIVEGGRDDFAPVLEA
                     IRACGSLDYARAAAARESQAAARAIEGLAGSSYKDSLLELASFSVTRAA"
     gene            1399..1713
                     /gene="rplU"
                     /locus_tag="VI319_07750"
     CDS             1399..1713
                     /gene="rplU"
                     /locus_tag="VI319_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY6863782.1"
                     /translation="MYAVIKSGGKQYRVESGAKLRIERLSADVGAAVSFEEVLLVGDG
                     DGVKVGAPLVSGAQVKATVLAQGRGDKVRIFKLRRRKHYQKTQGHRQSYTEVRIDDII
                     QG"
     gene            1720..1989
                     /gene="rpmA"
                     /locus_tag="VI319_07755"
     CDS             1720..1989
                     /gene="rpmA"
                     /locus_tag="VI319_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY6863783.1"
                     /translation="MAHKKAGGSSRNGRDSQAKRLGVKVFGGERVNAGQILVRQRGTR
                     VHAGENVGIGRDHTLYARVAGQVQYRVRGADNNNTVSVVKENAPA"
     gene            2048..>2550
                     /gene="obgE"
                     /locus_tag="VI319_07760"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             2048..>2550
                     /gene="obgE"
                     /locus_tag="VI319_07760"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616072.1"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY6863784.1"
                     /translation="MKFIDEAKIEVHAGKGGDGSAHFRREKYVPRGGPDGGDGGRGGS
                     VWARADRNLNTLIDYRFARIHRAKDGTRGGGSDCNGAAGADLELRVPVGTMIRDEETG
                     DPVADLARDGARALLARGGQGGLGNVHFKSSVNRAPRQFTRGEPGESRRLALELRVLA
                     DVGLLGLP"
BASE COUNT          407 a          897 c          884 g          362 t
ORIGIN      
        1 cttgtcgacc ccaagcgcga gctcgacgag atcttcagcg ccggcgagcg ggccggcatg
       61 ctgatctatc gtgacgacga cgagctcgtg cgcctggcgc gctcgctacc cgggcgcgcc
      121 accgagctgc gcgccgctgc gcgcgaggcc gcgccgtcct tccaccgcaa tcactcctat
      181 ctcgcgcgag tcgccgagat gatgcggatc ctcgccgcgc gcgcatgaga ccggctaccg
      241 gaatatcaag cagattggtc ggggtgagag gatttgaacc tccgacacct gcgtcccgaa
      301 cgcagtactc taccaggctg agctacaccc cgatgcttcg gccgctttga ctgtgacgct
      361 aggccactga ccgctaggcc gcgcgcgtca cagaaaagga cgccaattct agcaaagaat
      421 ccttgtagct cgacccggcg agcccctcga tcgcccgcgc ggccgcctgc gactcgcgcg
      481 ccgccgcggc tcgcgcgtag tcgagcgagc cgcaggcgcg gatcgcttcg agcaccggcg
      541 cgaagtcgtc gcggccgccc tcgacgatcg cgcgccgcac gagcccggcc tgcgccggcg
      601 tgccgttgcg caggacatgg ataagcggca gcgtcggctt gccttcggcg aggtcgtcgc
      661 ccaggctctt gccgatcgtc gccgcgtcgc ccgagtagtc gaggacgtcg tcgatcagct
      721 ggaaggccgt gccgacgtgc gccccgtagg cggcgaggcc gttctcgagc gccgcggggc
      781 tgcgcgcgag cacgccgccc agccgcgccg ccgcttcgaa gagacgcgcg gtcttgcgcc
      841 ggatcacctc gaggtatctc gcctcgtcca cctccgggtc gtggctcccc atgagctgca
      901 gcacctcgcc tgcggcgatg gtgttcgtgg cgtccgcgag cacgcgcatg acgcgcatgt
      961 cgtcgacctc gaccatcatc tggaaggcgc gcgagtagag aaaatcgccc accagcaccg
     1021 ccgccgcgtt gccgaacgcg gcgttcgccg tcggctggcc gcggcgcagg cgcgattcgt
     1081 ccaccacgtc gtcgtgcagc agggtcgcgg tgtggatgaa ctcgatgacc gcggcgagcg
     1141 cgaagcgcgc ctcggcgctt tttcccgcgg cgccggccgc gccgcaggcg agcaggagca
     1201 gcgcgggtcg cagccgcttg ccgcccgagg cgacgatgta ctgcgccacc tcccgcacca
     1261 ggggcacgtc ggattcgagg cgcgcaccga tcagctcgtc cacccgccgc aggtcgtcgg
     1321 cgacgggggc cagcatggac acgaagctca tgattccttt ataattcgcg ccctttcagg
     1381 cgtccagaaa gcaagcgaat gtacgcagtt atcaagtccg gcggcaaaca gtatcgcgtc
     1441 gagtccggcg cgaagctccg catcgagcgc ctttccgccg atgtcggcgc cgcggtttcg
     1501 ttcgaggagg tgctgctcgt cggcgacggc gacggcgtca aggtgggcgc cccgctcgtg
     1561 tcgggggcgc aggtcaaggc gaccgtcctg gcgcagggcc gcggcgacaa ggtgcgcatc
     1621 ttcaagctgc gccggcgcaa gcactaccag aaaacccagg gccatcggca gtcgtacacc
     1681 gaggtccgca tcgacgacat catccagggg tagtaggcca tggcacacaa gaaggcgggc
     1741 ggcagctcgc gcaacggacg cgactcgcag gcgaagcggc tcggcgtcaa ggtcttcggc
     1801 ggcgagcgcg tcaacgccgg ccagatcctc gttcgccagc gcggcacgcg cgtgcacgcg
     1861 ggcgagaacg tcggcatcgg ccgcgaccat accctctatg cgcgcgtcgc cgggcaggtg
     1921 cagtatcgcg ttcgcggcgc agacaacaac aacacggtaa gcgtcgtgaa ggaaaacgct
     1981 ccggcgtagc cggtcctcat ctcacaaaag ccctatcgag atcggtaggg cttttttgtt
     2041 tgtacagatg aaattcatcg acgaggcgaa gatcgaggtg catgcgggca agggcggcga
     2101 cggctcggcg cacttccggc gcgagaagta cgtgccgcgc ggcgggcccg acggcggcga
     2161 tggcgggcgc ggcggcagcg tgtgggcgcg cgccgatcgc aacctcaaca cgctcatcga
     2221 ctaccgtttt gcccgcatcc accgcgcgaa ggacggcacg cgcggcggcg gctccgactg
     2281 caacggcgcc gccggcgcgg acctcgagct gcgcgtgccc gtcggcacga tgatccgcga
     2341 cgaggaaacc ggcgatccgg tggccgacct cgcgcgcgac ggcgcgcggg cgctgctcgc
     2401 gcgcggcggc cagggcgggc tcggcaacgt gcatttcaag tcgagcgtca accgcgcgcc
     2461 ccggcagttc acgcgcggcg agccgggcga gagccgccgg ctcgcgctcg agctgcgcgt
     2521 gctcgccgac gtcggcctgc tcggcctgcc
//
LOCUS       DATDMW010000176         7608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1920005, whole genome shotgun sequence.
ACCESSION   DATDMW010000176 DATDMW010000000
VERSION     DATDMW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920005"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="VI319_07765"
     CDS             complement(<1..1684)
                     /locus_tag="VI319_07765"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY6863785.1"
                     /translation="MPGPKRARGPCDYNLPMAREVMSFDVVVVGAGPAGLGAAIRLKQ
                     LAAARNAEISVCVLEKGSEVGAHILSGAVMDPRALDELLPDWNSKGAPLRTPVTEDRF
                     LFLTASGALQVPSFLLPECFRNHGNYVVSLGNVCRWLARQAEALGVEIYPGFAAAEVL
                     YGARGNVVGVATGDMGLDREGKPTAQYQSGIELHGKYIFFAEGCRGHLGRRLIERYKL
                     DEHADPQTYGIGLKELWEIDAAKHRPGLVLHSAGWPLDASTYGGSFLYHMEERLVSIG
                     FVVGLSYRNPYLDPYQEFQRYKTHPRIRGFLEGGKRIAYGARALAAGGLVSLPKLVFP
                     GGALIGDDAGFLNASRIKGSHAAIKSGMLAAEAAADALAAGRAGDTLDAYPSAFEKSW
                     LFEELYRARNFKQWMAKGLYAGTMMVGFEQKLLGGKVPWTLHHAHADHECLEPAASHS
                     PIEYPKPDGKLSFDRPSSVFLSNTNHAENQPVHLTLKDPTVPLRIDLPEYAGPEQRYC
                     PAGVYEFVGDGEKRFQINSQNCVHCKTCDIKDPTQNIVWVTPQGGDGPNYPNM"
     gene            1701..3668
                     /locus_tag="VI319_07770"
     CDS             1701..3668
                     /locus_tag="VI319_07770"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEY6863786.1"
                     /translation="MSDQPLWQPTQERIEQANVTRFAREAIRVWGLGFNDYPSFYRWT
                     IEHPEQFWTSLWKFADVRAGLMGERVLVDGDRMPGARWFPDARLNFAENLLRRRDGGE
                     AVVFWGEDKVKRRLSYHDVYDRVSRVAQALRAEGVKAGDRVAGYLPNMPEALIATLAC
                     ASLGAIWSSCSPDFGVQGVLDRFGQIEPVVLFACDGYWYNGKRIDVLAKVRDIEAMLP
                     TVRRVVIAPYVSEHPDLSDVHGAVTFEKFVEPYRGGEIRFEQLPFEHPLYILFSSGTT
                     GVPKCIVHGAGGALLKHVCEQQLHSDVKPGDRLFYFTTLGWMMWNWLISGLASGATLV
                     LYDGSPFVNGGRILFDLADAERVTQFGTSAKFIDACAKAGLEPAKTYPLTSVRTVLST
                     GSPLVPEGFDYVYRALKRDACLSSISGGTDICGCFVLGNPILPVWRGEIQCRALGLAV
                     DVYDEKGRPVRGRKGELVCTRPFPSMPLGFWNDDDGSRYRAAYFEKFPGVWTHGDWAE
                     ITAHDGAIIYGRSDAVLNPGGVRIGTAEIYRQVEQLDEVVESLVIAQDWPPRNPTDVR
                     VVLFVKLREGLVIDEALIAKIKAHVRVNTTPRHVPAKVVQVPDIPRTKSNKIVELAVR
                     NVVHGEPVKNVEALANPEALEHFRDRPELLT"
     gene            3672..4418
                     /locus_tag="VI319_07775"
     CDS             3672..4418
                     /locus_tag="VI319_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY6863787.1"
                     /translation="MALSIEAATGTHIGDKKEQQDRLAVLPGSGAVLAVVADGMGGLT
                     GGSMAAEQVISTARQLFGAWSPASEPGEALLRAIAQEAHVAIRLARFTSEQEPHSTVV
                     ALLVHGERAHWVHSGDSRLYIFRGATPVFRTVDHSYVEQLHREGKISAAERGTHPQRN
                     YLVSCLGAREEPRVEVGAAGDLRAGDAFLLCSDGLWAYFTDAELGAALVALAPRDAAE
                     KLVAAARVRAKGNGDNLSLAIVKLAAGSGG"
     gene            4525..6174
                     /locus_tag="VI319_07780"
     CDS             4525..6174
                     /locus_tag="VI319_07780"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY6863788.1"
                     /translation="MTKYVFVSGGVVSSLGKGIAAASLAAILESRGIKVTLLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFQSAKMKKCNNFTTGQIYESVIKKE
                     RRGDYLGRTVQVIPHVTDEIKSFIQRGANGAEVAIVEIGGTVGDIESLPFLEAVRQMG
                     LELGERNTCYLHLTLVPYIKSAGEIKTKPTQHSVKELREIGIQPDALLCRSDRPVPED
                     EKKKIAMFTNVQPNAVISVWDVDSIYKIPWMLHQQMLDEIVCHKLNIIARPAELRPWK
                     KLVEALEHPQHEVTIAMVGKYVELTDSYKSLNEALVHAGIHTRSKVNIRYIDSEMIES
                     DGVELLKGVDAVLVPGGFGKRGVEGKIRAIQYARENRIPYLGICLGMQLAVIEFARNV
                     VGLAGANSTEFEPDTPHPVIALVTEWQDRDGRVERRSEKSDLGGTMRLGAQAAALEGG
                     SLARKIYGADTVNERHRHRYEVNNQYIERLKDKGLRVSGLTQREQLCEMVELPGHPWF
                     VGCQFHPEFTSTPRAGHPLFTAFVEAALQYQAGKTQTAVAA"
     gene            6176..7024
                     /gene="kdsA"
                     /locus_tag="VI319_07785"
     CDS             6176..7024
                     /gene="kdsA"
                     /locus_tag="VI319_07785"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY6863789.1"
                     /translation="MRLCGFEVGLDQPLFLIAGPCVVESRQMALDTAGALRDIARKAG
                     VPFVYKSSYDKANRSSGKSYRGLGMDKGLEILSDVKKQIGVPVLTDVHDSADVQAVAA
                     VVDVLQTPAFLCRQTDFIHAVAATGKPVNIKKGQFLSPGEMKNVVDKAREAAGRSDTI
                     LVCERGASFGYNNLVSDMRSLAIMRDTGCPVVFDATHSVQLPGGQGTASGGQREFVPV
                     LARAAVASGVAGVFMETHPDPDKAPSDGPNAWPLDRMAELLGTLKELDQLVKRRGFAE
                     QSLAKR"
     gene            7046..>7608
                     /gene="eno"
                     /locus_tag="VI319_07790"
     CDS             7046..>7608
                     /gene="eno"
                     /locus_tag="VI319_07790"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY6863790.1"
                     /translation="MSSIVDVVAREILDSRGNPTVEADVLIESGVMGRAAVPSGASTG
                     TREALELRDKDTSRYGGKGVLKAVEHINTEISEAIMGLDAQEQSFIDKTLIELDGTEN
                     KARLGANALLAVSMAVAKAAAEESGLPLYRYFGGSGGMQMPVPMMNVINGGAHANNSL
                     DIQEFMIVPVGASTFRDALRCGAEIFQA"
BASE COUNT         1241 a         2563 c         2640 g         1164 t
ORIGIN      
        1 acatgttcgg gtaattcggc ccgtcgccgc cctgcggcgt cacccagacg atgttctgcg
       61 tcgggtcctt gatgtcgcag gtcttgcagt gcacgcagtt ctgcgagttg atctggaatc
      121 tcttctcgcc gtcgccgacg aactcgtaca cgcccgccgg gcagtagcgc tgctcggggc
      181 ccgcgtactc gggcaggtcg atgcgcagcg gcaccgtcgg gtccttcagc gtcaggtgca
      241 ccggctggtt ctcggcgtgg ttggtgttcg agaggaacac ggacgacggc cggtcgaagc
      301 tgagcttgcc gtcgggcttc ggatattcga tcgggctgtg gctcgcggcg ggctccaggc
      361 actcgtggtc cgcgtgcgcg tggtgcagcg tccacggcac cttgccgccg agcagcttct
      421 gctcgaagcc gaccatcatc gtgccggcgt acaggccctt cgccatccac tgcttgaagt
      481 tgcgcgcgcg gtacagctcc tcgaacagcc acgacttctc gaacgccgaa ggataggcgt
      541 cgagcgtgtc gccggcgcgt cccgcggcga gcgcgtcggc ggcggcctcg gcggcgagca
      601 tgccggactt gatcgccgcg tggctgccct tgatccgcga agcgttgaga aacccggcgt
      661 cgtcgccgat cagcgcgccg cccgggaaca cgagcttcgg cagcgacacc aggccgcccg
      721 cggcgagggc gcgcgcgccg taggcgatgc gcttgccgcc ctcgaggaag ccgcggatgc
      781 gcgggtgcgt cttgtagcgc tggaattcct ggtacgggtc gagatacgga ttgcggtacg
      841 agagaccgac gacgaagccg atcgacacca ggcgctcctc catgtggtac aggaacgagc
      901 cgccgtaggt cgaggcgtcg agcggccagc cggccgagtg cagcaccagg cccggccggt
      961 gcttcgccgc gtcgatctcc cacaattcct tgagcccgat cccgtaggtc tgcggatcgg
     1021 catgctcgtc gagcttgtag cgctcgatca accgccgccc gaggtggccg cggcagcctt
     1081 cggcgaagaa gatgtacttg ccgtggagct cgatccccga ctgatactgg gcggtcggct
     1141 tgccttcgcg atcgaggccc atgtcgcccg tcgcgacgcc gaccacgttc ccgcgggcgc
     1201 cgtacaggac ctccgccgcg gcgaagccgg gatagatctc gacgccgagc gcctccgcct
     1261 gccgcgcgag ccagcggcag acgttcccca ggctcaccac gtaattcccg tggttgcgga
     1321 agcattccgg aaggagaaag ctcgggacct ggagcgcgcc gctcgcggtg aggaacagga
     1381 accggtcttc ggtgaccggc gtgcgcagcg gagcgccctt cgagttccag tcgggcagca
     1441 gctcgtcgag cgcgcgcgga tccatcacgg cgcccgagag gatgtgcgcg ccgacctccg
     1501 agcctttctc gagcacgcac accgagatct cggcattccg cgcggcagcg agctgcttca
     1561 ggcggatcgc cgctcccagg ccggccggcc cagcgccgac gaccacgacg tcgaagctca
     1621 tcacctcacg cgccatgggc aaattataat cgcacggtcc gcgcgcccgc ttcgggcccg
     1681 gcatccaccc gagagagccc ttgtccgacc agccgctctg gcagcccacc caggaacgga
     1741 tcgagcaggc gaacgtcacg cgcttcgcgc gcgaggcgat ccgcgtctgg ggcctcggct
     1801 tcaacgacta tccgtcgttc tatcgctgga cgatcgagca tccggagcaa ttctggacct
     1861 cgctgtggaa attcgccgac gtgcgcgcgg gcctgatggg cgagcgcgtc ctcgtcgacg
     1921 gcgaccgcat gcccggcgcg cgctggttcc ccgacgcgcg cctcaacttc gccgagaacc
     1981 tgctgcgccg gcgcgacggc ggcgaagcgg tcgtgttctg gggcgaggac aaggtgaagc
     2041 ggcgcctctc gtaccacgac gtctacgacc gcgtctcgcg cgtggcgcag gcgctgcgcg
     2101 ccgagggcgt gaaggcgggc gaccgcgtcg cgggctacct gccgaacatg cccgaggcgc
     2161 tgatcgcgac gctcgcctgc gcgagcctcg gcgcgatctg gtcgtcgtgc tcgcccgatt
     2221 tcggcgtgca gggcgtgctc gaccgcttcg ggcagatcga gccggtcgtg ctgttcgcgt
     2281 gcgacggcta ctggtacaac ggcaagcgca tcgacgtcct cgccaaggtg cgcgacatcg
     2341 aggcgatgct gccgacggtg cgccgggtgg tgatcgcgcc ctacgtgtcc gagcaccccg
     2401 acctgtccga cgtgcacggc gcggtgacgt tcgagaagtt cgtcgagccg taccgcggcg
     2461 gcgagatccg cttcgagcag ctgccgttcg agcatccgct gtacatcctg ttctcgtccg
     2521 ggacgaccgg cgtcccgaag tgcatcgtgc acggcgcggg cggggcgctc ctcaagcacg
     2581 tctgcgagca gcagctgcac agcgacgtca agccgggcga ccggctgttc tacttcacca
     2641 cgctcggctg gatgatgtgg aactggctga tctcggggct cgcgtcgggc gcgacgctcg
     2701 tgctctacga cggctcgccg ttcgtgaacg gcggccggat cctgttcgac ctggccgacg
     2761 ccgagcgcgt gacgcaattc ggcacgtcgg cgaagttcat cgacgcctgc gcgaaggcgg
     2821 gcctcgagcc ggcgaagacg tatcccctga cgtcggtgcg gaccgtgctg tcgaccggct
     2881 cgccgctcgt gcccgagggc ttcgactacg tgtaccgcgc gctgaagcgc gacgcgtgcc
     2941 tgtcgtcgat ctcgggcggc accgacatct gcggctgctt cgtgctcggc aatccgatcc
     3001 tgccggtatg gcgcggggag atccagtgcc gggcgctggg gctcgcggtc gacgtctacg
     3061 acgagaaggg gcgccccgtg cgcggccgca agggcgagct ggtgtgcacg cggccgtttc
     3121 cctcgatgcc gctcggcttc tggaacgacg acgacggctc gcgctaccgc gccgcgtact
     3181 tcgagaagtt tcccggcgtg tggacccacg gcgactgggc cgagatcacg gcgcacgacg
     3241 gcgcgatcat ctacgggcgc tccgacgccg tgctcaatcc cggcggcgtg cgtatcggca
     3301 ccgcggagat ctaccggcag gtcgagcagc tcgacgaggt ggtcgagtcg ctggtgatcg
     3361 cccaggactg gccgccgcgc aacccgaccg acgtgcgcgt ggtgctgttc gtgaagctgc
     3421 gcgagggcct ggtgatcgac gaggcgctga tcgcgaagat caaggcacac gtgcgcgtca
     3481 acacgacgcc gcgccacgtc cccgcgaagg tcgtgcaggt gcccgacatc ccgcgcacca
     3541 agtcgaacaa gatcgtcgag ctcgccgtgc gcaacgtggt gcacggggag ccggtgaaga
     3601 acgtcgaggc gctcgccaat cccgaggcgc tcgagcactt ccgcgaccgc cccgagctgc
     3661 tgacctgatc catggccctc tccatcgagg ccgccaccgg cacccacatc ggcgacaaga
     3721 aggagcagca ggaccggctc gcggtgctcc cggggtcggg cgcggtgctc gcggtggtcg
     3781 cggacggcat gggcgggctc accggcggct cgatggcggc ggagcaggtg atcagcaccg
     3841 cgaggcagct tttcggcgcg tggtcgcccg cgagcgagcc cggcgaggcg ctgctgcgcg
     3901 cgatcgcgca ggaggcgcac gtggcgatcc gtctcgcgcg cttcacgagc gagcaggagc
     3961 cgcacagcac ggtcgtggcg ctgctcgtcc acggcgagcg cgcgcactgg gtgcactcgg
     4021 gcgacagccg cctgtacatt ttccgcggag cgacgccggt cttccgcacc gtggaccatt
     4081 cgtacgtcga gcagctgcac cgcgagggga agatctccgc tgccgagcgc gggacgcatc
     4141 cccagcggaa ctacctggtg agctgcctcg gcgcgcgcga ggagccgcgc gtcgaggtcg
     4201 gcgccgccgg agacctgcgg gccggggacg ccttcctgct gtgcagcgac ggcctttggg
     4261 cctacttcac cgatgccgag ctgggcgccg cgctcgttgc gctcgctccg cgcgacgcgg
     4321 cggagaagct ggtcgccgcg gcgcgcgtgc gggcgaaagg caacggcgac aatctctcgc
     4381 tcgcgatcgt gaagctcgcg gcggggagcg gcggctgagc ggccatattg tttccgcagc
     4441 ggcaacggcg ccgcggaacg gctgcgagcg agcgaggtcc gattttttca tgtaccattg
     4501 gaaatcccat ctccccggtg gtagatgact aagtatgtgt tcgtgtcggg cggtgtggtt
     4561 tcctctctgg ggaaggggat agccgccgcc tccctcgccg ccatcctcga atcgcgcggc
     4621 atcaaggtca ccctcctcaa gctcgacccg tacatcaacg tcgatcccgg cacgatgtcg
     4681 cctttccagc acggagaggt gttcgtcacc gaggacggcg ccgagaccga cctggacctc
     4741 ggacactacg agcgcttcca gtcggcgaag atgaagaagt gcaacaactt caccacgggc
     4801 cagatctacg agtcggtgat caagaaggag cgccggggcg actacctcgg ccgcaccgtg
     4861 caggtcatcc cgcacgtcac cgacgagatc aagagcttca tccagcgcgg cgcgaacggc
     4921 gccgaggtcg cgatcgtcga gatcggcggc acggtcggcg acatcgagtc gctgccgttc
     4981 ctggaggccg tgcgccagat gggcctcgag ctcggcgagc gcaacacctg ctacctgcac
     5041 ctgacgctcg tcccgtacat caagagcgcc ggcgagatca agaccaagcc gacgcagcac
     5101 tcggtcaagg agctgcgcga gatcgggatc cagcccgacg cgctgctgtg ccggtccgac
     5161 cgtcccgtcc ccgaggacga gaagaagaag atcgcgatgt tcacgaacgt ccagccgaac
     5221 gccgtcatct cggtctggga cgtggactcg atctacaaga ttccctggat gctgcaccag
     5281 cagatgctcg acgagatcgt ctgccacaag ctgaacatca tcgccaggcc ggccgagctg
     5341 cgtccgtgga agaagctcgt ggaggcgctc gagcacccgc agcacgaggt cacgatcgcc
     5401 atggtcggca agtacgtcga gctcaccgac tcgtacaagt cgctgaacga ggcgctggtg
     5461 cacgccggca tccatactcg cagcaaggtg aacatccgct acatcgactc ggagatgatc
     5521 gagtcggacg gcgtcgagct gctgaagggc gtcgacgcgg tgctcgtgcc gggcggcttc
     5581 ggcaagcgcg gcgtcgaagg caagatccgc gcgatccagt acgcgcgcga gaatcgcatt
     5641 ccgtacctcg gcatctgcct gggcatgcag ctcgcggtga tcgagttcgc ccggaacgtc
     5701 gtcggcctcg ccggcgcgaa cagcacggag ttcgagccgg atacgccgca cccggtcatc
     5761 gcgctcgtta ccgagtggca ggatcgcgac ggccgcgtcg agcgccggag cgagaaatcc
     5821 gatctcggcg gcaccatgcg actcggggcg caggcggccg cgctcgaagg cggctcgctc
     5881 gcgcgcaaga tctacggcgc cgacaccgtg aacgagcggc accggcaccg gtacgaggtc
     5941 aacaaccagt atatcgagcg gctgaaggac aagggactgc gcgtctcggg cctcacgcag
     6001 cgcgagcagc tgtgcgagat ggtcgagctg ccggggcatc cgtggttcgt cggctgccag
     6061 ttccatccgg agttcacgtc cacgccgcgc gccgggcatc cgctgttcac cgcgttcgtc
     6121 gaggccgcgc tgcaatacca ggcggggaag acgcaaaccg ctgtcgccgc ctgaaatgag
     6181 actttgcggg ttcgaagtcg gcctcgacca accgctgttc ctgatcgccg gcccctgcgt
     6241 ggtggagtcc cggcagatgg cgctcgacac cgcgggagcg ctgcgcgaca tcgcccgcaa
     6301 ggccggcgtg ccgttcgtgt acaagtcgtc ctacgacaag gcgaaccgca gctcggggaa
     6361 atcgtaccgc gggctcggaa tggacaaagg actcgagatc ctgtccgacg tgaagaagca
     6421 gatcggcgtc ccggtgctca cggacgtgca cgactccgcc gatgtccagg cggtcgcggc
     6481 ggttgtcgac gtgctccaga ctcctgcgtt cctctgtcgc cagaccgact tcatccacgc
     6541 ggttgccgcg accgggaagc ccgtgaacat caagaaaggc cagtttcttt cgccggggga
     6601 aatgaaaaac gtggtggaca aggcgcgaga ggccgccggc cgcagcgaca cgatcctcgt
     6661 atgcgagcgc ggggcttcgt tcggctacaa caacctcgtc tccgacatgc gctcgctcgc
     6721 gatcatgcgc gacacgggct gcccggtcgt gttcgacgcg acgcactcgg tgcagctgcc
     6781 gggcgggcag gggaccgcct ccggcggcca gcgcgagttc gtcccggtgc tggctcgcgc
     6841 ggccgtcgcg agcggcgtcg cgggcgtgtt catggagacg catcccgacc cggacaaggc
     6901 gccgtccgac gggccgaacg cctggccgct ggaccgcatg gcggagctgc tcggcacgct
     6961 gaaggagctg gaccagctgg tgaagcggcg gggattcgcc gagcagtcgc tggcgaagcg
     7021 atgaacatcg aacaaggatc gcgacatgag ctccatcgtt gacgtcgtcg caagggagat
     7081 cctcgactca cgcggcaatc ccacggtcga ggccgacgta ctgatcgagt cgggggtgat
     7141 gggccgcgcc gccgtgccgt cgggcgcctc gaccgggacg cgcgaggcgc tcgagctgcg
     7201 cgacaaggac acgagccgct acggcggcaa gggcgtgctg aaagccgtcg agcacatcaa
     7261 caccgagatc tccgaggcca tcatgggcct cgacgcccag gagcagagct tcatcgacaa
     7321 gacgctgatc gagctcgacg gcaccgagaa caaggcgcgg ctcggcgcga acgcgctgct
     7381 ggcggtgtcg atggccgtcg ccaaggccgc ggccgaggaa tcggggctgc cgctgtaccg
     7441 ctacttcggc ggctcgggcg gcatgcagat gcccgtgccg atgatgaacg tcatcaacgg
     7501 cggcgcgcac gcgaacaaca gcctcgacat ccaggagttc atgatcgtgc cggtcggcgc
     7561 gtcgacgttc cgcgacgccc tgcgctgcgg cgccgagatc ttccaggc
//
LOCUS       DATDMW010000177        13989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2293678, whole genome shotgun sequence.
ACCESSION   DATDMW010000177 DATDMW010000000
VERSION     DATDMW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13989
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293678"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2722
                     /locus_tag="VI319_07795"
     CDS             <1..2722
                     /locus_tag="VI319_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6863791.1"
                     /translation="GRPRGGSSAGSGEVRGNVRPPARARADLAEDGDPSHIGSRVLLG
                     LEHEVERALANAESLRVAVRSVIRVVCETLGWDCGRLLRVDEAAGVLRFFEHWSVEGP
                     DIARFIERSRQITHAPGKGLSGHVWQSGAPIWVTDLTKDPRVQYGLALDTVMHGAFHF
                     PVTAQGKVIGVLSFNSREVRPPDEQLLQAIRVIGSLVGQFLQRADTEDVLRRSEERFR
                     HLTEMSSDWYWEQDEGFRFTMVSENLARRTKRPPHLVYGKTRWELPTLNMSTEDWLRH
                     RALLEEHQPYRNLELHRLDADGNDMYFSVSGEPIFDAQGRFRGYRGVGIDITARKQAE
                     EALRQSEVRYRSVVDHLAEGVYIIDAERRIVSCNASAERILGVARESLLGKTLAEVNF
                     KVTREDGTPFPAEDRPASKTLLTGEPQSGVVMGVERADGSVIWISMSTRMLPGAGQGA
                     PSGVVMSFNDITERRSFEEKLTYLAQYDLLTGLPNRALLVDRLDQALIRARRQEAMVG
                     VLLIDLDRFKEINDSMGHSAGDAVLKEVARRVRKALRDTDSVARLGGDEFCIVIEGAE
                     SRSHVSVAAAKIRRLFEEPVLTSENKEIFIELSIGSSVYPEDGETMEDLLKNADIAMY
                     EAKRDGGHAFRFYSSALRAKTADEINLVSMLRRAVDRNELVLYYQPQIDIATGRPVGV
                     EALVRWEHPELGLVSPYRFVRLAEETGLIVPIGEWVLKTACAEAKAWQLAGMPEMKVS
                     VNRSARQFRDSQLIRKIAAVLAATGLPPRLLELEITESVIMSHTDHTIGVLQRLTELG
                     VRVSVDDFGTGYSSLAYLKRFPVHKLKIDLAFVRDITTNKEDAAIVQAIVTLARTLKL
                     RVIAEGVETDEQLKHLASIGCHEYQGFVYSRPVPARELIELVRKQFVGMF"
     gene            complement(2726..3565)
                     /locus_tag="VI319_07800"
     CDS             complement(2726..3565)
                     /locus_tag="VI319_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY6863792.1"
                     /translation="MDMRAAIVSRAALFALAAVTVLAGCETVQTTQPGVVGVDRKQHF
                     LVSSDQMNAAAEDAYKKTIADARAKHQLDTNAAHVEQVKRVVSRLIPNTGYFRPDAPK
                     WAWEFHVLSTDEVNAWCMPGGKIAVYSGLLDKVKPSDDELAAVLGHEIAHALREHSRE
                     QASQAMAEQIGLGVLGAGLGLSDVTQQLSGMLLKVGFELPHSRTDETEADRVGLELAA
                     RSGYDPHAAVSLWQKMEQVAGGGGGGGIQKFLSDHPPTEQRLADMKANADKLQPVYVA
                     AKK"
     gene            3652..6612
                     /locus_tag="VI319_07805"
     CDS             3652..6612
                     /locus_tag="VI319_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863793.1"
                     /translation="MSPRIPRGAYFALFTVSGFAGLVYESIWSHYLRLFLGHAAYAQT
                     LVLALFMGGMAIGAWICSRASSRWTNLLRGYAIAEALIGVAALAFHAVYVGATEAAYS
                     SVLPALGNAAAAATFKWTLAGLLILPQSVLLGMTFPLMSAGLIRRYPAAPGESLALLY
                     FTNSLGASLGVLASGFVMIERFGLPGTVQLAGLVNLALAAAVWLLAGRDEPRPRAAAA
                     APRDDASGAPWRLLLAVAALTGAASFVYEIGWIRMLSLVLGSATHSFELMLSAFILGL
                     ACGGLWVRGRIDALGDPARTLGVVQVAMGLAALATLPLYGRMFEVMQAVIRGLAKTDS
                     GYAMFLLASHGIALAIMFPATFCAGMTLPLITYALLRGGHGERSIGAVYGANTLGSIA
                     GVFAAAHLGMPLLGLKGLIAAGAALDAALGVALLWRAAGARRLGFGAAALTVAAFAAV
                     LVAAPLDAYKMASGVFRRGDLYTEREARLLSQRDGKTATVSLVDFGSDRSLRTNGKSD
                     GAVNMDPGGPRISDEITMTLTGALPLAIRPDARRVAVIGIGTGLTTHTLLGSSTVESV
                     ETIEIEPAMAEASRRFAPRNSNTFADPRSHIVFDDAKTFFSTHNRKYDIVVSEPSNPW
                     VSGVSSLFTAEFYRHVRRYLEPGGVLVQWFQLYEIDPSLVASVLVALGSEFPDYAVYA
                     ATNADLLIVAGDAATLARPLADLLAMPGVARELRRVHVASMDDLRIRRIGGKAALAPL
                     FASYGVPPNSDFRPYLDLHAAKYRFLQQSAGELVGLGLAAVPAVAMLEGASERGTPAP
                     TELGDEFLDKAEHIRRARYARDFLLRPVPPEPQGIPRQLQKDLELVHARLIECRDPGK
                     YDIWLHALYQLAREVNPLLGADDSAAVWDRVERAPCARDRSAEPRAWLALFRAVSRRD
                     AAAMAAGAETLLAVPSDLPAGSRQYLVAAGMAGYLAQGRRAKAAELLARTPREPGGSD
                     ELTLRLLAAHALGK"
     gene            6639..6956
                     /locus_tag="VI319_07810"
     CDS             6639..6956
                     /locus_tag="VI319_07810"
                     /inference="COORDINATES: protein motif:HMM:NF020469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEY6863794.1"
                     /translation="MLIRFHSKTDGFTIQGDLGVALLRLMGMSGTVPGALLAEDVPQA
                     LERLRQGVASGKPIEAPAADASEAGYRISLATRAFPLVQLLENAVKRSSAVVWEELRN
                     HTG"
     gene            complement(6967..7629)
                     /gene="lepB"
                     /locus_tag="VI319_07815"
     CDS             complement(6967..7629)
                     /gene="lepB"
                     /locus_tag="VI319_07815"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY6863795.1"
                     /translation="MRKWIRDNRGWIIFFALFGLMRTAVADWNYVPSASMRPTLLEGD
                     LVFVDRVAYDLKLPFTEIALAHLGEPRRGDIVVFSSPKDGVRLIKRLVAVPGDTVEMR
                     DKRLVINGRPAEYAPRGWVTEPLEGAPVRALRLAETTPDGERTIQWLGSTPRDSFAPF
                     TVPQGHYLMLGDNRDDSADSRYFGLVDRRLLTGRAERVLLSADVLGDWLPRFGRFGRS
                     LR"
     gene            complement(7629..8729)
                     /gene="aroC"
                     /locus_tag="VI319_07820"
     CDS             complement(7629..8729)
                     /gene="aroC"
                     /locus_tag="VI319_07820"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY6863796.1"
                     /translation="MSGNTFGVLYCVTSFGESHGPAYGGVIDGCPPGLALSEADIQRE
                     LDRRKPGTSRHVTQRQESDRIEILSGIFEGKTTGTAIGFVIRNEDQRSKDYSALADTF
                     RPGHADYTYWQKYGLRDYRGGGRASARETMIRVAAGAVAKKWLRERYGVEVRGYLAQL
                     GPHRVPFKSWDGVNDNPFFVADPSAVAKLEAFMDELRSSGDSVGARINVVASGVPVGW
                     GEPVYGRLDSDIAAAMMGINAVKGVEIGAGFASIEQKGSQHGDELTPQGFLSNHAGGV
                     LGGISTGQDVAVSIALKPTSSIRLPRRSIDRKGNPTTVETKGRHDPCVGIRATPIAEA
                     MLACVLMDHALRHRAQCGDVTPPLKPIPGKTR"
     gene            8756..8941
                     /locus_tag="VI319_07825"
     CDS             8756..8941
                     /locus_tag="VI319_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863797.1"
                     /translation="MADDDPDKLKGPGGLTLRQIHEQVQKSVAKDREAQKMRNQLAQK
                     PSRWQRFVRYVWDKKRG"
     gene            complement(8943..9938)
                     /locus_tag="VI319_07830"
     CDS             complement(8943..9938)
                     /locus_tag="VI319_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6863798.1"
                     /translation="MRIAIAFLALPGALALLALLAAWAITSMPGRSHRGPLPPLEPAD
                     AELASRLRAHVTAIASEEHNVIRAEALERSARYIERELARGGPVVRDEFSVAGAKVRN
                     LEVVVPGSDAQAPVLVIGAHYDSVRHAVGANDNGSGVAALIELARALREKPPGNAEIR
                     LVAFVNEESPWFGTDAMGSLRHARALARSGRRVLGMLSLETIGWYSDEPGSQKYPPPF
                     SSLYPREGNFIGFVGNFKSRDFVRRVVASFRAHAAFPAEGGAFPDAIKDAGRSDQWSY
                     WQQGWPGLMVTDTANFRYPYYHTMQDTPDKVDYERTARVVRGLEAVIRDLASGAG"
     gene            complement(9938..11245)
                     /locus_tag="VI319_07835"
     CDS             complement(9938..11245)
                     /locus_tag="VI319_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY6863799.1"
                     /translation="MARIRFPGGKFAFEPTRRQALAAAGALGAAALTGATWHLWPEQG
                     FSNPCEGTLPRELAEHELVLAAWDGLDASQCWDAHVHLAGMGDSDSGIVVNPRMTSLA
                     HPVQFARRRFFLNAGCATSGDGVDKAYVARLRALAEGMRPGFKLLLLPFECNVGENGQ
                     VSWERTSFHVPNRWAAQVAYRDVVRFEWAASIHPYRHDAVERLHAAVAGGARAVKWLP
                     AAMGIDPASPICDPFYYALARAKMPLISHAGMELAVDVESDAFGNPLRLRRALEHGVR
                     VVVAHCASAGTDVDLDRGASGPVVPSFELFSRMMDDPRWEGTLFADISTLTQINRVGP
                     PLARVLERGEWHARLLNGSDYPLPGVMPLYSLERLVELGFLERSAAPVLTAIRAHNAL
                     LFDFVLKRSLRRQGRRFADGVFRTRPFFEPPAERPLRAGQHRA"
     gene            complement(11233..11667)
                     /locus_tag="VI319_07840"
     CDS             complement(11233..11667)
                     /locus_tag="VI319_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6863800.1"
                     /translation="MQVSEILRVKGNVLYTIPMEGSAIDAVKAMADHNMGSLVVFDKG
                     RMVGLLTFHEVLGAMSDRDGRLDGVRVGDIMQRDPVTATPKMEFDQLRRVMLETGARY
                     LPVMDGGDLLGVISFRDVAKAVLEEQDFENRMLKGYIKNWPE"
     gene            11796..12773
                     /locus_tag="VI319_07845"
     CDS             11796..12773
                     /locus_tag="VI319_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863801.1"
                     /translation="MRFSTGFFVAAVVSALALASGPAFPQDYPTKPIRLVVPFAPGGS
                     SSIIARAFSTEMAKGLGQSFVVENKPGGAGNVAMDDVARAAPDGYTLIIGHIGTLAVN
                     PFMFAKLPFDTNRDFRAVSLFAKVPTIFVVHESVPAKDLVEFIALAKKEPGKLYYGSA
                     GNGSAGHLAIEYLKLVSGMEIQHVPYKGTGPNLIDLVAGRTQFSAAGTPPFLPHVKSG
                     KLRVVAVGTTKRLPILPEVKTVAEQGYPGFETSQWYGLNAPAKTPDAIVSRLARESAK
                     AARSADVQRQLEPDAAEAIGSTPEEYERYIKSEQARWSEVVRKAHIKAD"
     gene            12776..>13989
                     /gene="tcuA"
                     /locus_tag="VI319_07850"
     CDS             12776..>13989
                     /gene="tcuA"
                     /locus_tag="VI319_07850"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEY6863802.1"
                     /translation="MAAEYDVVVVGGGNAALCAALSAKEKAQRVLVLERAPEEESGGN
                     SRFTAGLMRIVYNGPDDLAQLFELSAEERERTDFGTYTEAQFLDDMARVTEYRCDPDL
                     TEILVKQSFPTALWMRKKGVRFTAAWGRQAFKIGGKFKFWGGLTLEAIGGGPGLVENL
                     TAAAKKYGIEIWYQSRATALLADDAGVHGVKVRRNGKTVDVKAKAVVLAAGGFQANTE
                     MRTRYLGPGWELAKVRGTRFNTGDAIRMALDVGAAPVGNWSGCHAVAWERNAPEFGDL
                     AVGDQFQKHSYPWGIYINAEGKRFVDEGADFRNYTYAKYGRVILNQPLQFAWQIFDAK
                     VKSQLRDEYRIKQVTKVTANTLEELVQKLDDVNAEQALKEIKAYNAAVKQDIPFNPNV
                     KDGRRTEGLAVN"
BASE COUNT         2060 a         4915 c         4990 g         2024 t
ORIGIN      
        1 aggccgccct cgggggggaa gcagcgcggg atcgggcgag gtgcgcggca acgtgcgccc
       61 gcccgcgcgc gcgcgggccg acctcgccga ggacggcgac ccctcgcaca tcggcagccg
      121 cgtcctgctc ggcctcgagc acgaggtcga gcgcgccctc gccaacgcgg agagcctgcg
      181 cgtcgcggtc aggtccgtga tccgcgtcgt gtgcgagacc ctcggctggg actgcgggcg
      241 gctcctgcgc gtcgacgagg cggccggcgt gctgcgcttt ttcgagcact ggagcgtcga
      301 gggccccgac atcgcgcgct tcatcgagcg ctcgcgtcag atcacccacg cgcccggcaa
      361 ggggctctcg ggacacgtgt ggcagtcggg cgcgccgatc tgggtcaccg acctcacgaa
      421 agacccgcgc gtgcagtacg gcctcgcgct cgacacggtg atgcacggcg ccttccactt
      481 ccccgtcacg gcccagggca aggtgatcgg cgtcctgtcc ttcaacagcc gcgaggtgcg
      541 cccgccggac gagcagctgc tgcaggcgat ccgcgtgatc ggcagcctgg tcgggcagtt
      601 cctgcagcgc gccgacaccg aggatgtcct gcgccgcagc gaggagcgct tccgtcacct
      661 caccgagatg tcctcggact ggtactggga gcaggacgag ggcttccggt tcacgatggt
      721 ctcggagaac ctcgcgcggc ggaccaagcg tccgccccac ctcgtctacg gcaagacgcg
      781 ctgggagctg ccgacgctga acatgagcac cgaggactgg ctgcggcacc gcgcgctgct
      841 cgaggagcac cagccgtacc gcaacctcga gctgcaccgg ctcgacgccg acggcaacga
      901 catgtacttc agcgtgagcg gggagccgat cttcgacgcg caaggccgct tccgcggcta
      961 tcgcggcgtg ggcatcgaca tcaccgcgcg caagcaggcc gaggaggcgc tccggcagag
     1021 cgaggtgcgc taccgctcgg tggtcgatca cctcgccgag ggcgtgtaca tcatcgacgc
     1081 cgagcggcgc atcgtcagct gcaacgcgag cgccgagcgc atcctcggcg tcgccaggga
     1141 gtcgctgctc ggcaagacgc tggcggaggt gaatttcaag gtcacgcgcg aggacgggac
     1201 gccgtttccg gccgaggacc gcccggccag caagacgctg ctcaccggcg agccgcagtc
     1261 gggcgtcgtc atgggcgtgg agcgcgcgga cggctcggtc atctggatct cgatgagcac
     1321 ccgcatgctc cccggcgccg gtcagggcgc gccgagcggg gtggtgatgt cgttcaacga
     1381 catcaccgag cggcgcagct tcgaggagaa gctcacctac ctcgcgcagt acgacctgct
     1441 gaccggcctg ccgaaccgcg cgctcctggt cgaccggctg gaccaggcgc tcatccgggc
     1501 gcgccggcag gaggcgatgg tcggcgtgct gctcatcgac ctcgaccggt tcaaggagat
     1561 caacgactcg atgggccatt ccgcgggcga cgcggtgctg aaggaggtcg cgcgccgggt
     1621 gcgcaaggcg ctgcgcgaca ccgactcggt ggcgcggctg ggcggcgacg agttctgcat
     1681 cgtgatcgag ggcgccgaga gccgctcgca cgtctcggtc gccgccgcca agatccggcg
     1741 gctgttcgaa gagccggtgc tgacctcgga gaacaaggag atcttcatcg agctctcgat
     1801 cggctcgagc gtctaccccg aggacggcga gacgatggag gacctgctca agaacgccga
     1861 catcgcgatg tacgaggcca agcgcgacgg cgggcacgcc ttccgcttct actcgagcgc
     1921 gctgcgcgcg aagaccgccg acgagatcaa cctcgtgtcg atgctgcgcc gcgcggtcga
     1981 ccgcaacgag ctggtgctct actaccagcc gcagatcgac atcgcgaccg ggcgcccggt
     2041 cggcgtcgag gcgctcgtgc gctgggagca tcccgagctc ggcctggtct cgccgtaccg
     2101 gttcgtgcgg ctggccgagg agacggggct catcgtgccg atcggcgagt gggtgctgaa
     2161 gaccgcctgc gccgaggcga aggcgtggca gctcgccggg atgcccgaga tgaaggtgtc
     2221 ggtcaaccgg tcggcgcgcc agttccgcga cagccagctc atccgcaaga tcgccgcggt
     2281 gctcgcggcg accggcctgc cgccgcggct gctcgagctg gagatcaccg agagcgtcat
     2341 catgagccac accgatcaca cgatcggcgt gctgcagcgc ctgaccgagc tcggcgtgcg
     2401 cgtctcggtc gacgacttcg gcaccggcta ctcgtcgctc gcgtacctca agcgcttccc
     2461 ggtgcacaag ctcaagatcg acctcgcctt cgtgcgcgac atcacgacca acaaggaaga
     2521 cgcggcgatc gtgcaggcga tcgtcacgct cgcgcgcacg ctgaagctgc gcgtcatcgc
     2581 cgaaggcgtg gagaccgacg agcagctgaa gcacctggcc tcgatcggct gccacgagta
     2641 ccagggcttc gtctacagcc gccccgtgcc ggcgcgcgag ctgatcgagc tcgtgcgcaa
     2701 gcagttcgtc gggatgttct gagcgctact tcttcgccgc gacgtacacc ggctgcagct
     2761 tgtccgcgtt ggccttcatg tcggcgaggc gctgctccgt cggcgggtgg tcggacagga
     2821 acttctggat cccgccgcct ccgccgccgc ctgcgacctg ctccatcttc tgccacagcg
     2881 agaccgcggc gtgcgggtcg tagcccgagc gcgccgcgag ctccaggccg acgcggtcgg
     2941 cctcggtctc gtcggtgcgc gagtgcggca gctcgaagcc gactttgagc agcatgccgc
     3001 tcagctgctg cgtcacgtcg gagagcccca ggcccgcgcc cagcacgccg agcccgatct
     3061 gctcggccat cgcctgcgac gcctgctcgc ggctgtgctc gcgcagcgcg tgcgcgatct
     3121 cgtgcccgag gacggcggcg agctcgtcgt cgctcggctt gaccttgtcg agcagccccg
     3181 agtacacggc gatcttgccg cccggcatgc accaggcgtt cacctcgtcg gtcgacagca
     3241 cgtggaactc ccaggcccat ttcggcgcgt cggggcggaa atagccggtg ttcgggatga
     3301 ggcggctcac cacgcgcttg acctgctcga cgtgcgcggc gttggtatcg agctggtgct
     3361 tggcccgcgc atcggcgatc gtcttcttgt acgcgtcctc ggcggccgcg ttcatctggt
     3421 cggaggacac caggaagtgc tgcttgcggt cgacgccgac cacgccgggc tgcgtagtct
     3481 gaacggtctc acagcccgcc agaacggtaa cggccgcgag cgcgaagagc gccgcccggc
     3541 tcacgattgc tgcgcgcatg tccatggatc gacacctctt catcggttag ctgcgcgttg
     3601 gacaaggcgg agcgccgctt ggtttttctt ccggttacaa tcgccgcgcc catgagcccc
     3661 cggattccgc gcggcgctta cttcgccctg ttcaccgtct cgggcttcgc cgggctcgtc
     3721 tacgagtcga tctggagcca ttacctgcgg ctgttcctcg ggcacgcggc gtacgcgcag
     3781 acgctggtgc tcgcgctgtt catgggcggg atggcgatcg gcgcgtggat ctgcagccgc
     3841 gcctcgagcc gctggacgaa cctgctgcgc ggctacgcga tcgcggaagc gctgatcggc
     3901 gtcgcggcgc tcgccttcca tgccgtctac gtcggcgcca cggaggccgc gtactcgagc
     3961 gtgctgcccg cgctcggcaa cgccgccgcg gcggcgacgt tcaagtggac gctcgccggc
     4021 ctgctgatcc tgccgcagtc ggtgctgctc gggatgacgt tcccgctgat gagcgcgggg
     4081 ctgatccggc gctacccggc cgcgcccggc gagtcgctcg cgctgctcta tttcacgaac
     4141 agcctcgggg cgtcgctcgg cgtgctcgcg agcggcttcg tgatgatcga gcgcttcggg
     4201 ctgccgggga cggtgcagct cgcgggcctg gtgaacctcg cgctcgccgc cgccgtctgg
     4261 ctgctcgcgg ggcgcgacga gccgcggccg cgggcggctg cggccgcgcc gcgcgacgac
     4321 gcgagcggcg cgccgtggcg cctgctgctc gcggtcgcgg cgctcaccgg cgcggcctcg
     4381 ttcgtctacg agatcggctg gatccggatg ctctcgctcg tgctgggcag cgcgacgcac
     4441 tccttcgagc tgatgctctc ggcgttcatc ctcggcctcg cctgcggcgg cctgtgggtg
     4501 cgcggccgca tcgacgcgct cggcgacccg gcgcggacgc tcggcgtggt gcaggtcgcg
     4561 atggggctcg ccgcgctcgc gacgctgccg ctctacggcc ggatgttcga ggtgatgcag
     4621 gcggtgatcc ggggcctcgc gaagacggat tcgggctacg ccatgttcct cctcgcgagc
     4681 cacggcatcg cgctcgcgat catgttcccg gcgacgttct gcgccgggat gacgctcccg
     4741 ctcatcacct acgcgctgct gcgcggcggc cacggcgagc gctcgatcgg cgcggtctac
     4801 ggcgcgaaca cgctcggctc gatcgcgggc gtgttcgccg cggcgcatct cgggatgccg
     4861 ctcctcggcc tgaaaggcct gatcgcggcg ggcgcggcgc tcgacgcggc gctcggcgtc
     4921 gcgctgctgt ggcgcgccgc cggcgcgcgt cggctcggct tcggcgcggc cgcgctcacc
     4981 gtcgccgcct tcgccgccgt gctggtcgcg gcgcccctcg acgcctacaa gatggcgtcg
     5041 ggcgtgttcc gccgcggcga cctgtatacg gagcgcgagg cgcgcctgct ctcgcagcgc
     5101 gacggcaaga cggcgaccgt gagcctggtc gacttcggca gcgatcggag cctgcgcacc
     5161 aacggcaagt cggacggcgc ggtcaacatg gatcccggcg gcccgcgcat ctccgacgag
     5221 atcacgatga cgctgacggg cgcgctgccg ctcgcgatcc ggcccgacgc gcggcgggtc
     5281 gccgtgatcg gcatcggcac cggcctcacc acgcacacgc tgctcggctc ctcgacggtc
     5341 gagtcggtcg agacgatcga gatcgagccg gcgatggccg aggcgtcgcg gcgcttcgcc
     5401 ccgcgcaact cgaacacctt cgccgatccg cgcagccaca tcgtgttcga cgacgcgaag
     5461 accttcttct cgacgcacaa ccgcaagtac gacatcgtgg tctcggagcc gtcgaacccg
     5521 tgggtgagcg gggtctcgag cctgttcacc gccgagttct accggcacgt gcgccgctac
     5581 ctcgagccgg gcggcgtcct ggtgcagtgg ttccagctct acgagatcga cccgagcctg
     5641 gtcgcctccg tgctggtcgc gctcggatcc gagttccccg actacgccgt ctacgcggcg
     5701 accaacgccg acctgctgat cgtcgccggc gacgcggcga cgctcgcgcg cccgctcgcc
     5761 gacctgctcg cgatgccggg cgtcgcgcgc gagctgcggc gcgtccacgt cgcgtcgatg
     5821 gacgacctgc gcatccgcag gatcggcggc aaggcggcgc tcgcgccgct gttcgcgagc
     5881 tacggcgtcc cgccgaactc ggatttccgg ccttacctcg acctgcacgc ggcgaagtac
     5941 cgcttcctgc agcagtcggc gggcgagctg gtcggcttgg ggctcgccgc cgtgcccgcg
     6001 gtcgcgatgc tcgaaggcgc gagcgagcgc ggcacgcccg cgccgaccga gctcggcgac
     6061 gagttcctcg acaaggccga gcacatccgg cgcgcgcgct acgcgcgcga cttcctgctg
     6121 cgtcccgtgc cgcccgagcc gcagggcatc ccgcgccagc tgcagaagga cctcgagctc
     6181 gtccacgcgc gcctcatcga gtgccgcgat cccgggaagt acgacatctg gctgcacgcg
     6241 ctgtaccagc tcgcgcgcga ggtgaatccc ctgctcggcg ccgacgactc cgcggcggtc
     6301 tgggaccggg tcgagcgcgc gccgtgcgcg cgcgaccggt cggccgagcc gcgcgcgtgg
     6361 ctcgccctct tccgggcggt gagccggcgc gatgccgccg cgatggcggc cggcgccgag
     6421 acgctgctcg cggtcccgag cgacctgccg gcggggtcgc gccagtacct ggtcgccgcc
     6481 gggatggcgg gctacctcgc gcagggccgg cgcgcgaagg ccgccgagct gctggcgcgc
     6541 acgccgcgcg agccgggcgg cagcgacgag ctgacgctgc gcctgctcgc cgcgcacgcg
     6601 ctcgggaaat aatttccgcc ggacgcggtc caacggccat gctcatccga ttccacagca
     6661 agaccgacgg cttcaccatc cagggcgatc tcggcgtcgc gctgcttcgc ctcatgggca
     6721 tgagcggcac ggtgccgggc gcgctgctcg ccgaggacgt gccccaggcg ctcgagcgcc
     6781 tccggcaggg ggtcgcgtcc ggcaagccga tcgaggcgcc ggccgccgac gcgtcggagg
     6841 ccggctaccg catcagcctg gcgacccgcg cgtttcccct ggtgcagctg ctggagaatg
     6901 ccgtcaagcg cagctcggcg gtcgtctggg aggagctccg gaaccacacg gggtgaatgt
     6961 cgggcgtcag cgcagcgacc gcccgaagcg cccgaaccgc ggcagccagt cgcccagcac
     7021 gtcggccgag agcagcacgc gctcggcgcg cccggtgagc agccgccggt cgaccaggcc
     7081 gaagtagcgc gagtcggcgc tgtcgtcgcg gttatcgccg agcatcaggt agtggccctg
     7141 cgggacggtg aaaggcgcga acgaatcgcg cggcgtggat cccagccact ggatcgtgcg
     7201 ctcgccgtcg ggcgtcgtct cggcgaggcg cagcgcgcgc accggcgcgc cttcgagcgg
     7261 ctcggtcacc cagccgcgcg gcgcgtactc ggccgggcgc ccgttgatga cgaggcgctt
     7321 gtcgcgcatc tcgaccgtgt cgcccggcac cgcgaccagg cgcttgatca gccgcacgcc
     7381 gtccttcggc gaggagaaca cgacgatatc gccgcgccgg ggctcgccga gatgcgcgag
     7441 ggcgatctcc gtgaacggca gcttcagatc gtaggcgacg cggtcgacga agaccaggtc
     7501 gccctcgagc agcgtcgggc gcatcgacgc cgagggcacg tagttccaat cggcgaccgc
     7561 ggtgcgcatc aggccgaaga gcgcgaagaa gatgatccag ccgcggttgt cgcggatcca
     7621 ctttctcatc atcgtgtctt tccgggaatc ggtttcaaag gcggcgtgac gtcgccgcac
     7681 tgcgcgcggt gccgcagcgc gtggtccatg agcacgcacg cgagcatggc ctcggcgatc
     7741 ggcgttgcgc ggatgccgac gcagggatcg tggcggcctt tggtctcgac ggtcgtggga
     7801 ttgcccttgc ggtcgatcga gcgccgcggc aggcggatgc tcgaggtcgg cttgagcgcg
     7861 atcgagacgg cgacgtcctg cccggtcgag atgccgccga gcacgccgcc cgcgtggttc
     7921 gagaggaagc cctgcggcgt cagctcgtcg ccgtgctgcg agcctttctg ctcgatcgac
     7981 gcgaaccccg cgccgatctc gacgcccttc accgcgttga tgcccatcat cgccgccgcg
     8041 atgtccgagt cgagccgccc gtacaccggc tcgccccagc cgacggggac gcccgaggcg
     8101 acgacgttga tgcgcgcgcc gaccgagtcg cccgagctgc gcagctcgtc catgaacgct
     8161 tcgagcttcg ccaccgccga agggtcggcg acgaagaaag gattgtcgtt cacgccgtcc
     8221 cacgacttga acggcacgcg gtgcggcccg agctgcgcga ggtagccgcg cacctcgacg
     8281 ccgtagcgct cgcgcagcca cttcttcgcg accgcgcccg cggcgacgcg gatcatcgtc
     8341 tcgcgcgccg acgcgcgccc gccgccgcgg tagtcgcgca gcccgtattt ctgccagtac
     8401 gtgtagtcgg cgtggccggg gcggaacgtg tcggcgagcg ccgagtaatc cttcgagcgc
     8461 tggtcctcgt tgcggatcac gaagccgatc gcggtgccgg tggtcttgcc ttcgaagatc
     8521 ccggaaagga tctcgatgcg gtcggactcc tggcgctgcg tgacgtggcg cgaggtgccc
     8581 ggcttcctgc ggtcgagctc gcgctggatg tcggcctccg agagcgcgag ccccggcggg
     8641 cagccgtcga tcacgccgcc gtacgcgggc ccgtggctct cgccgaacga ggtcacgcag
     8701 tagaggacgc cgaacgtgtt gccggacatg tgtgtcgaag tctaccatcg aagccatggc
     8761 ggacgacgac cccgacaagc tcaaaggccc cggcggactg acgctgcgcc agatccacga
     8821 gcaggtgcag aagtcggtcg cgaaggaccg cgaggcgcag aagatgcgca atcagctcgc
     8881 gcagaagccg agccggtggc agaggttcgt ccgctacgtc tgggacaaga agcgcggctg
     8941 actcagcccg cgccggaagc gagatcgcgg atcaccgcct cgagcccgcg caccacgcgc
     9001 gcggtgcgct cgtagtcgac cttgtcgggc gtgtcctgca tcgtgtggta gtacgggtag
     9061 cggaagttcg cggtgtcggt gaccatgagg ccgggccagc cctgctgcca gtacgaccac
     9121 tggtccgagc gccccgcgtc cttgatggcg tcggggaagg cgccgccttc ggccgggaac
     9181 gcggcgtgcg cgcggaacga ggcgacgacg cggcgcacga agtcgcgcga cttgaagttg
     9241 ccgacgaagc cgatgaagtt gccctcgcgc ggatagagcg agctgaacgg cggcgggtat
     9301 ttctggctgc cgggctcgtc gctgtaccag ccgatggtct cgagcgagag catgccgagc
     9361 acgcgccggc ccgatcgcgc gagcgcgcgc gcatggcgca ggctgcccat cgcgtcggtg
     9421 ccgaaccacg gcgactcctc gttgacgaac gcgaccaggc ggatctccgc atttcccggc
     9481 ggcttctcgc gcagcgcgcg cgccagctcg atcagcgccg ccacgcccga gccgttgtcg
     9541 ttcgcgccga ccgcgtggcg caccgagtcg tagtgcgcgc cgatcacgag cacgggcgcc
     9601 tgggcgtcgc tccccgggac gacgacctcg aggttgcgca ccttcgcgcc cgcgacgctg
     9661 aactcgtccc gcacgaccgg cccgccgcgc gcgagctcgc gctcgatgta gcgcgccgag
     9721 cgctcgagcg cctcggcgcg gatgacgttg tgctcctcgc tcgcgatcgc ggtcacgtgc
     9781 gcgcgcaggc gcgaagcgag ctccgcgtcg gcgggctcga gcggcggcag cggcccgcgg
     9841 tgcgagcgcc cgggcatcga cgtgatcgcc cacgcggcga gcagcgcgag gagcgcgagc
     9901 gcgccgggaa gcgcgaggaa ggcgatcgcg atgcgcatca ggcgcggtgc tgcccggcgc
     9961 gcagcggccg ctcggccggc ggctcgaaga aggggcgcgt gcggaacacg ccgtcggcga
    10021 agcgccggcc ctgccggcgg aggctccgct tgagcacgaa gtcgaagagc agcgcgttgt
    10081 gcgcgcggat cgcggtgagc acgggcgcgg ccgagcgctc gaggaagccg agctcgacca
    10141 ggcgctcgag cgagtacagc ggcatcacgc ccggcagcgg gtagtcggag ccgttcagga
    10201 ggcgcgcgtg ccactcgccg cgctcgagca cgcgcgcgag cggcggcccg acgcggttga
    10261 tctgcgtcag cgtcgagatg tcggcgaaca gcgtcccctc ccagcgcgga tcgtccatca
    10321 tgcgcgagaa cagctcgaag ctcgggacga ccggcccgct cgcgccgcgg tcgaggtcga
    10381 cgtcggtccc ggccgaggcg cagtgcgcga ccacgacgcg cacgccgtgc tcgagcgcgc
    10441 gccgcagccg caaggggttg ccgaaggcgt ccgactcgac gtcgaccgcc agctccatgc
    10501 cggcgtggct gatgagcggc atcttcgcgc gcgcgagcgc gtagtagaac gggtcgcaga
    10561 tcggcgatgc gggatcgatg cccatcgccg cgggcagcca cttcaccgcg cgcgccccgc
    10621 ccgcgaccgc cgcgtgcagc cgctcgaccg cgtcgtggcg gtaggggtgg atcgacgccg
    10681 cccactcgaa gcgcacgacg tcgcggtacg cgacctgcgc cgcccagcgg ttggggacgt
    10741 ggaaggaggt gcgctcccac gagacctggc cgttctcgcc gacgttgcac tcgaacggca
    10801 gcagcagcag cttgaacccg ggccgcatgc cctcggcgag cgcgcgcagg cgcgcgacgt
    10861 acgccttgtc gacgccgtcg ccgctcgttg cgcagccggc gttgaggaag aagcgccgcc
    10921 gcgcgaactg caccgggtgc gcgaggctgg tcatgcgcgg attgacgacg atgccgctgt
    10981 cggaatcgcc catgccggcg aggtgcacgt gcgcgtccca gcactgcgac gcgtcgagcc
    11041 cgtcccaggc ggcgagcacc agctcgtgct cggccagctc gcgcggcagc gtgccctcgc
    11101 acggattgct gaagccctgc tcgggccaga ggtgccaggt cgcgccggtc agcgcggcgg
    11161 cgccgagcgc gcccgcggcg gcgagcgcct gccggcgggt cggctcgaag gcgaacttac
    11221 cgccggggaa tcttattcgg gccaattctt gatgtagccc ttcagcatgc ggttctcgaa
    11281 gtcctgctcc tcgagcacgg ccttggcgac gtcgcggaag gagatgacgc cgagcaggtc
    11341 gccgccgtcc atgacgggga ggtagcgcgc gccggtctcg agcatgacgc ggcgcagctg
    11401 gtcgaactcc atcttcggcg tcgcggtgac cgggtcgcgc tgcatgatgt cgccgacgcg
    11461 cacgccgtcg aggcgcccgt cgcggtcgct catcgcgccg agcacctcgt ggaaggtgag
    11521 gaggcccacc atgcggcctt tgtcgaagac gacgagcgac cccatgttgt ggtcggccat
    11581 ggccttgacg gcgtcgatcg cgctgccctc catcgggatg gtgtacagga cgttcccctt
    11641 gaccctcagg atctcgctca cttgcatggc cgtctccttg gtgggctccc tggcgaaaat
    11701 catacgcccg ggcgcagcgt gccgccaccc gcgcctctgg cgccggtccg cgaccgcgtg
    11761 gtaagttcgg cgttccccga tgagaggata aattcgtgcg tttctccacc ggctttttcg
    11821 tcgcagcggt cgtgtcggcg ctcgccttgg cgtccggccc cgcgttcccc caggattacc
    11881 ccaccaagcc gatccgcctc gtcgtcccgt tcgcgcccgg cggcagctcg tcgatcatcg
    11941 cgcgcgcctt ctcgaccgag atggcgaagg ggctcggcca gtcgttcgtg gtcgagaaca
    12001 agccgggcgg cgcgggcaac gtcgcgatgg acgacgtcgc gcgcgccgcg cccgacggct
    12061 acacgctgat catcggccac atcgggacgc tcgcggtgaa cccgttcatg ttcgcgaagc
    12121 tgccgttcga cacgaaccgc gatttccgcg cggtgtcgct gttcgcgaag gtgccgacga
    12181 tcttcgtcgt gcacgagagc gtgccggcga aggacctcgt ggaattcatc gcgctcgcga
    12241 agaaggagcc ggggaagctc tactacggct cggccgggaa cggctcggcg gggcatctcg
    12301 cgatcgagta cctgaagctc gtcagcggga tggagatcca gcacgtgccg tacaagggca
    12361 cgggcccgaa cctgatcgac ctcgtcgccg ggcgcacgca gttctccgcg gccggcacgc
    12421 cgccgttcct gccgcacgtg aagtcgggga agctgcgcgt ggtggcggtc gggaccacca
    12481 agagactccc gatcctgccc gaggtgaaga ccgtcgccga gcagggctat cccggcttcg
    12541 agacgtcgca gtggtacggg ctgaacgcgc cggcgaagac gcccgatgcg atcgtgagcc
    12601 ggctcgcgcg cgagtcggcg aaggcggcga ggagcgccga cgtgcaaagg cagctcgagc
    12661 ccgacgccgc cgaggcgatc ggctcgacgc cggaggagta cgagcgctac atcaagagcg
    12721 agcaggcgcg ctggagcgag gtcgtgcgca aggcgcacat caaggcggac tgacgatggc
    12781 cgccgagtac gacgtggtgg tggtgggcgg gggcaacgcg gcgctgtgcg ccgcgctctc
    12841 ggcgaaggag aaggcgcagc gcgtgctcgt gctcgagcgc gcgcccgagg aggagtcggg
    12901 cgggaattcc cggttcaccg ccggcctgat gcgcatcgtc tacaacgggc cggacgacct
    12961 cgcgcagctc ttcgagctct cggccgagga gcgcgagcgc accgacttcg ggacgtatac
    13021 ggaagcgcag ttcctcgacg acatggcgcg cgtcaccgag tatcgctgcg accccgacct
    13081 gaccgagatc ctggtgaagc agagcttccc gaccgcgctc tggatgcgca agaaaggcgt
    13141 gaggttcacg gcggcgtggg gccggcaggc gttcaagatc ggcggcaagt tcaagttctg
    13201 gggcggcctc acgctggaag cgatcggcgg cggcccgggc ctggtcgaga acctcaccgc
    13261 cgccgcgaag aagtacggca tcgagatctg gtaccagtcg cgcgcgaccg ccctcctcgc
    13321 cgacgacgcc ggcgtccacg gcgtgaaagt gagacgcaac ggcaagaccg tcgacgtcaa
    13381 ggcgaaggcc gtggtgctcg ccgccggcgg cttccaggcc aacaccgaga tgcgcacccg
    13441 ctacctcggc cccggctggg agctcgcgaa agtgcgcggc acgagattca acaccggcga
    13501 cgccatccgc atggcgttgg acgtgggagc ggcgcccgta ggcaactgga gcggctgcca
    13561 cgccgtcgcc tgggagcgca acgcccccga gttcggcgac ctcgccgtcg gcgaccagtt
    13621 ccagaagcac tcgtatccct ggggcatcta catcaacgcc gagggcaaga gattcgtcga
    13681 cgaaggcgcc gacttccgca actacaccta cgcgaaatac ggacgggtga tcctgaatca
    13741 gccgctgcag ttcgcctggc agatcttcga cgcgaaagtg aagagccagc tgcgggacga
    13801 gtaccgcatc aagcaggtca cgaaggtcac cgccaacacg ttggaagagc tcgtgcagaa
    13861 gctcgacgac gtcaacgccg agcaagcgct gaaagagatc aaggcctaca acgccgcggt
    13921 caagcaggac atccccttca accccaacgt caaggacggc cgccgcaccg aaggcctcgc
    13981 tgtaaacaa
//
LOCUS       DATDMW010000178         5695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_179362, whole genome shotgun sequence.
ACCESSION   DATDMW010000178 DATDMW010000000
VERSION     DATDMW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179362"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VI319_07855"
     CDS             <1..1353
                     /locus_tag="VI319_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6863803.1"
                     /translation="FSVVNPGTKADKLAWLLDKYRATALVTEGRLLPVAAEAIPRAPS
                     LVLALVAGARDLPAGLVDWDAALAAAPETPVRDRGIDVDLAMIVTTSGSTGVPKGVMM
                     THQNVVAAATSITTYLQSSPEDRVLSVLPLAFDYGLYQVLMSVKVGATLVLEKSFTYP
                     SLVLQKLRQERITGLPVVPTMVAILLQMKDIRPRDFPDLRTITSTAAALPPAHIQRLQ
                     DLFPATRLYSMYGLTECKRCTWLPPEELRRRPGSVGIAIPGTEAWIVDESGRRVGPGV
                     TGELVIRGAHVFKGYWENPEETDKMLKPGPWPWEKVLHTGDLFKADADGYLYFVGRKD
                     DIIKTAGEKVSPKEVENVIYALPGIRHAAVIGVPDAVLGQAIRAVVVAEPDSGLTERG
                     VIAHCAAHLESFMVPGAVEFRSELPQGPTGKIRRSQLQAEAARRVLGLPAADAEAVAP
                     "
     gene            1350..2360
                     /gene="nadE"
                     /locus_tag="VI319_07860"
     CDS             1350..2360
                     /gene="nadE"
                     /locus_tag="VI319_07860"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEY6863804.1"
                     /translation="MTHVYSDLRDIAPALAFSAHALDIDPETVADDIAAVLREQVLKH
                     LRKRGVVVGLSGGVDSSVVAALAVRALGPEHVFGLVTPEQDSEPDSLRLGRGVAEAFG
                     IESALEDITPILAAAGCYRRRDEFIREVFPEYTPEFRCKLVLPDVLAGRGYNVHQLVI
                     ESPDGTQHRARPPLHVALGIVAATNMKQRTRKQIEYYHADRLNYAVAGTPNRLEYDQG
                     FFVKNGDGAADVKPIAHLYKTQVYRLAEFLGVPAEVRRRPPTTDTYSLAQTQEEFFFS
                     VPLAKLDLCLYALDHSVPASETAPATELSEDQVERVYDDIRRKRASARYQHTPPLLVE
                     TV"
     gene            2357..3064
                     /locus_tag="VI319_07865"
     CDS             2357..3064
                     /locus_tag="VI319_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY6863805.1"
                     /translation="MRSLPVLRRFHVPAIRLPEWCHLIHTDYRRYRATEESALTTIFL
                     TQGFWASCAYRFSRGILHGMRPGVPKNILKTFTALLQKTVEIVTGICIPRECNIGEGF
                     YIGHYGTIILPRFGRIGHNCNVGQNVTIGIVVKGAKRWTPTIGNRVFIGAHSVIIGNL
                     TIGDDAMICAGSVVTRSVPARGVVMGNPAKIISYEGSFDYISYDGMESDTDRRVSLEA
                     ARKQAASAADPAVSAAA"
     gene            3114..3890
                     /locus_tag="VI319_07870"
     CDS             3114..3890
                     /locus_tag="VI319_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY6863806.1"
                     /translation="MAVQSLRKGKTVRQQVSKEEWGKRVDLAAAYRLMDVYGMTEMSA
                     NHISTRVPGEENAFLINPYGLLYDQMVASCFIKLDLAGNVLFNPTELGFNKAGYVIHG
                     AIHGARHDVDCVIHTHTVAGMAVSAMKCGLMPIAQTSMRFAKIAYHDYQGVAIDEAEQ
                     ASLVRDLGEHAGMILRNHGLLVVGGSIAEAFNNIFRLERACQVQVAALSCNVELQLPP
                     QDVIDASYRLYLPQTRRPFGVLEWPALLRKLDKIDPSYRE"
     gene            3901..4680
                     /locus_tag="VI319_07875"
     CDS             3901..4680
                     /locus_tag="VI319_07875"
                     /inference="COORDINATES: protein motif:HMM:NF004796.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY6863807.1"
                     /translation="MTTELLHEVRDGVAYVTFNRPQARNALTFAMYDRLAEICNAANE
                     DRSLRAILITGGGDKAFAAGTDISQFRAFDKEEDALAYEARIDRVMNAIERCRVPTVA
                     AIHGACTGGGASIAACCDLRIASREMKYGFPVARTLGNCLSLASYSRLVYLVGPALVK
                     DIVFRARLIEADEALDKGLVSELCDDQAALLKRAEEIVRTVASHAPLTLQATKEAVLR
                     LRPEVPHGAGNDLVLMCYMSQDFREGMEAFLGKRKPVWKGK"
     gene            4796..5467
                     /locus_tag="VI319_07880"
     CDS             4796..5467
                     /locus_tag="VI319_07880"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY6863808.1"
                     /translation="MAQWASIAVVAAFAGCSTINRGHDPSDVSKTEVSRAQATLEEFR
                     NDTQMAGFRERLKDAKGVVIAPRLGRGGFILGGGGAEAVALLREPGGRWSSPAFYKLA
                     SASVGLQAGADVSEVVMLLMTDKAVNALLNPKFDLGADASVAAGPVGVGAQRDVTADV
                     LSYARSRGAYAGLSVGGSYIQPDEAANKAYYGKAVTPTDILVRRNVSNPESASLQRSL
                     SRMPG"
     gene            5488..>5695
                     /locus_tag="VI319_07885"
     CDS             5488..>5695
                     /locus_tag="VI319_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863809.1"
                     /translation="MAKSKDLQGEGNYDAAREFDQAEEKFVKSGKVEQAARDAEPKNE
                     KEKEEIERAEEEARKRAKGEDPQVK"
BASE COUNT          944 a         1967 c         1981 g          803 t
ORIGIN      
        1 ttcagcgtgg tgaacccggg caccaaagcc gacaagctcg cctggctgct cgacaagtac
       61 cgcgcgacgg cgctggtcac cgagggccgg ttgctcccgg tcgcggccga ggccatcccg
      121 cgcgcgccgt cgctcgtgct cgcgctggtc gcgggcgcgc gcgacctgcc cgccggtctg
      181 gtcgactggg acgcggcgct cgccgcggcg cccgagacgc cggtgcgcga ccgcggcatc
      241 gacgtcgact tggcgatgat cgtcacgacc tcgggctcga ccggcgtgcc caagggcgtg
      301 atgatgaccc accagaacgt ggtcgccgcc gcgacctcga tcacgaccta cctgcagagc
      361 tcgcccgagg accgcgtgct gtcggtgctg ccgctcgcct tcgactacgg gctgtaccag
      421 gtgctgatga gcgtgaaggt cggcgcgacg ctcgtgctcg agaaatcctt cacctatccg
      481 tcgctcgtcc tgcagaagct caggcaggag cgcatcaccg ggctgccggt cgtgccgacc
      541 atggtcgcga tcctgctgca gatgaaggac atccgccccc gcgattttcc cgacctgcgc
      601 accatcacca gcaccgcggc cgcgctgccg cccgcgcaca tccagcggct gcaggacctg
      661 ttccccgcga cgcgcctgta ctcgatgtac ggcctgaccg aatgcaagcg ctgcacctgg
      721 ctgccgcccg aggagctgcg gcggcgtccg ggctcggtcg ggatcgccat ccccggaacc
      781 gaggcgtgga tcgtcgacga gagcggccgc cgcgtcggac ccggcgtgac cggcgagctc
      841 gtgatccgcg gcgcgcacgt gttcaagggc tactgggaga acccggagga gaccgacaag
      901 atgctgaagc ccggcccctg gccgtgggag aaggtgctcc acaccggcga cctgttcaaa
      961 gccgacgccg acggctatct ctacttcgtc gggcgcaagg atgacatcat caagaccgcg
     1021 ggagagaagg tgagcccgaa ggaggtcgag aacgtgatct acgcgctgcc gggcatccgg
     1081 cacgcggcgg tgatcggcgt tccagacgcg gtgctcgggc aggcgatccg cgcggtcgtc
     1141 gtcgccgagc ccgactcggg cctgaccgag cgcggcgtca tcgcgcactg cgccgcgcac
     1201 ctcgagagct tcatggtgcc cggcgcggtc gagttccgca gcgagctgcc gcagggcccg
     1261 acgggcaaga tccgccgcag ccagctgcag gcggaggccg cgcgccgggt gctcgggctt
     1321 cccgccgccg acgcggaggc ggtcgcgcca tgacccatgt ctattcggac ctgcgcgaca
     1381 tcgcgccggc gctcgctttc tcggcgcacg cgctcgacat cgacccggaa acggtcgccg
     1441 acgacatcgc cgccgtgctg cgcgagcagg tgctcaagca cctgcgcaag cgcggcgtgg
     1501 tggtgggtct ttcgggcggc gtcgactcga gcgtggtcgc cgcgctcgcc gtgcgcgcgc
     1561 tcgggcccga gcacgtgttc gggctggtca cgcccgagca ggactcggag cccgacagcc
     1621 tgcgcctcgg gcgcggggtc gccgaggctt tcggcatcga gagcgcgctc gaggacatca
     1681 cgccgatcct cgccgccgcc ggctgctacc gcaggcgcga cgagttcatc cgcgaggtgt
     1741 tccccgagta cacgccggag ttcaggtgca agctggtgct ccccgacgtg ctcgcgggcc
     1801 gcggctacaa cgtgcaccag ctcgtgatcg agtcgcccga cgggacgcag caccgcgcgc
     1861 gcccgccgct gcacgtcgcg ctcggcatcg tcgccgcgac caacatgaag cagcgcacgc
     1921 gcaagcagat cgagtactac cacgccgacc gcctgaacta cgcggtcgcc gggacgccca
     1981 accgcctcga gtacgaccag ggcttcttcg tcaagaacgg agacggcgcg gccgacgtga
     2041 agccgatcgc gcacctgtac aagacgcagg tgtaccggct ggccgagttc ctcggcgtgc
     2101 ccgccgaggt gcgccggcgg ccgccgacca ccgacacgta ctcgctcgcg cagacgcagg
     2161 aagagttctt cttctcggtg ccgctcgcca agctcgacct gtgcctgtac gcgctcgacc
     2221 attcggtgcc cgcgtccgag acggctcccg cgacggagct ctccgaggac caggtggagc
     2281 gcgtctacga cgacatccgt cgcaaacgcg cgagcgcgcg ctaccagcac acgccgccgc
     2341 tcctggtgga gacggtatga ggagcctgcc ggtgctgcgc cgctttcacg tgcccgccat
     2401 ccgcctgccg gaatggtgcc acctcatcca taccgactac cggcgctacc gggcgaccga
     2461 ggagtcggcg ctgaccacga tctttctcac gcagggcttc tgggcgagct gcgcgtaccg
     2521 cttctcgcgc gggatcctgc acggcatgcg gccgggcgtc ccgaagaaca tcctgaagac
     2581 gttcaccgcg ctgctgcaga agacggtgga gatcgtcacc gggatctgca tcccgcgcga
     2641 gtgcaacatc ggcgagggct tctacatcgg gcactacggc acgatcatcc tgccgcgctt
     2701 cggccgcatc ggccacaact gcaacgtcgg gcagaacgtg accatcggta tcgtggtgaa
     2761 aggcgcgaag cgctggacgc cgacgatcgg caaccgcgtg ttcatcgggg cgcactccgt
     2821 gatcatcggc aacctcacca tcggcgacga cgccatgatc tgcgccggct cggtggtgac
     2881 gcgctcggtg ccggcgcgcg gggtggtgat gggcaacccc gcgaagatca tctcgtacga
     2941 aggcagcttc gactacatca gctacgacgg catggagagc gacaccgacc gccgcgtctc
     3001 gctcgaggcg gcgcgcaagc aggccgcttc cgccgccgat ccggccgtct cggccgcggc
     3061 ctgaaaatcg cgcgcgctgt atacttctcc ggttccccgc cggagaagct gccatggccg
     3121 tccagtccct gaggaaaggc aagaccgtcc gccagcaggt cagcaaggag gagtggggca
     3181 agcgcgtcga cctcgcggcc gcgtaccggt tgatggacgt gtacggcatg accgagatga
     3241 gcgcgaacca catcagcacg cgcgtcccgg gcgaggagaa cgcgttcctg atcaacccgt
     3301 acgggctgct gtatgaccag atggtcgcgt cgtgcttcat caagctcgac ctggcgggca
     3361 acgtgctctt caacccgacc gagctcggtt tcaacaaggc gggctacgtg atccacggcg
     3421 cgatccacgg cgcgcgccac gacgtcgact gcgtgatcca cacgcacacg gtcgcgggca
     3481 tggcggtgtc ggcgatgaag tgcgggctga tgccgatcgc gcagacgtcg atgcgcttcg
     3541 cgaagatcgc ctaccacgac taccagggcg tcgcgatcga cgaggccgag caggcgagcc
     3601 tggtgcgcga cctgggcgag cacgccggga tgatcctgcg caaccacggg ctgctcgtgg
     3661 tcggcggctc gatcgccgag gcgttcaaca acatcttccg cctcgagcgc gcctgccagg
     3721 tgcaggtcgc ggcgctctca tgcaacgtcg agctgcagct gccgccgcag gacgtgatcg
     3781 acgcgtcgta ccggctgtac ctgccgcaga cgcgccggcc gttcggcgtg ctggagtggc
     3841 cggcgctgct ccggaagctc gacaagatcg atccctcgta ccgcgaatga gagagaagaa
     3901 atgaccaccg agctgctgca cgaggtgcgc gacggcgtcg cgtacgtcac cttcaaccgc
     3961 ccgcaggcgc gcaacgcgct caccttcgcg atgtacgacc ggctcgccga gatctgcaac
     4021 gcggcgaacg aggaccgctc gctgcgcgcg atcctgatca cgggcggcgg cgacaaggcg
     4081 ttcgcggccg ggaccgacat ctcgcagttc cgcgccttcg acaaggagga ggacgcgctc
     4141 gcgtacgagg cgaggatcga ccgcgtgatg aacgcgatcg agcgctgccg cgtgccgacg
     4201 gtggccgcga tccacggcgc gtgcacgggc ggcggcgcga gcatcgccgc gtgctgcgac
     4261 ctgcgcatcg cctcgcgcga gatgaagtac ggcttcccgg tcgctcgcac gctcggcaac
     4321 tgcctgtcgc tcgcgagcta ttcgcgcctg gtgtacctcg tcgggcccgc gctggtgaag
     4381 gacatcgtgt ttcgcgcgcg cctgatcgag gccgacgagg cgctcgacaa gggcctcgtg
     4441 agcgagctgt gcgacgacca ggccgctctg ctcaagcgcg ccgaggagat cgtgcgcacc
     4501 gtcgcgagcc acgcgccgct cacgctgcag gcgaccaagg aggcggtcct gcgccttcgt
     4561 cccgaggtgc cgcacggcgc gggcaacgat ctggtgctga tgtgctacat gagccaggat
     4621 ttccgcgagg ggatggaggc gttcctcggc aagcgcaagc ccgtctggaa gggcaagtag
     4681 tccgttcggc gcagccggcc ggtcgagatc ggccggattt tccgggcgtc gcggtgtcca
     4741 actcagcgtc gaacaacgct cgaggaggca caccatgaca ccccaagtaa gacgaatggc
     4801 gcagtgggca tcgatcgccg tggtcgcggc gttcgccggc tgctcgacga tcaaccgcgg
     4861 gcacgacccg agcgacgtca gcaagaccga ggtgagccgc gcgcaggcga cgctcgagga
     4921 attccgcaac gatacgcaga tggccggctt ccgcgagcgg ctgaaagacg ccaaaggcgt
     4981 cgtgatcgct ccgcgcctgg gccgcggcgg attcatcctg ggcggcggcg gcgccgaggc
     5041 cgtggcgctc ttgcgcgagc cgggcggccg gtggtcgagc ccggcgttct acaagctcgc
     5101 ctcggcgagc gtcggcctgc aggccggcgc cgacgtgtcc gaggtcgtca tgctgctcat
     5161 gaccgacaag gccgtgaacg cgctgctcaa cccgaagttc gacctcggcg ccgacgcgag
     5221 cgtcgccgcg gggccggtgg gcgtcggcgc gcagcgcgac gtgacggcgg acgtgctttc
     5281 gtacgccagg tcgcgcggtg cgtacgccgg cctctcggtc ggcggctcgt acatccagcc
     5341 cgacgaggcg gcgaacaagg cctactacgg caaggccgtc acgccgaccg acatcctcgt
     5401 ccgccgcaac gtgagcaacc ccgaatcggc gtcgctgcag cgctcgctca gccgcatgcc
     5461 cggttgagct tcgacaggag agacgagatg gcgaagagca aggacctgca gggcgaaggc
     5521 aactacgacg cggcgcgcga gttcgaccag gccgaggaga aattcgtgaa gtccggcaag
     5581 gtcgagcagg ctgcgcgcga cgcggagccg aagaacgaga aggagaagga agagatcgag
     5641 cgcgccgagg aagaggcgcg gaagcgcgcg aagggcgagg atccgcaggt gaagc
//
LOCUS       DATDMW010000179        13007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1547944, whole genome shotgun sequence.
ACCESSION   DATDMW010000179 DATDMW010000000
VERSION     DATDMW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547944"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VI319_07890"
     CDS             <1..698
                     /locus_tag="VI319_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6863810.1"
                     /translation="SGTKTWISNAPIADLFVVWAKDEAGAICGLLLERGWAGLSAPEI
                     HGKIGLRASITGEIVMDDVFVPDSHRLPDAAGIKAPFACLNSARYGIAWGALGAAEDC
                     WHRARQYVLDRKQFGRPLAANQLVQKKLADMQTEIALGLQGCLRLGRLKDEGADAPEM
                     TSILKRNSCGKSLDIARLARDMLGGNGISDEFGVVRHMLNLEVVNTYEGTHDIHALIL
                     GRAQTGIQAFSPE"
     gene            796..999
                     /locus_tag="VI319_07895"
     CDS             796..999
                     /locus_tag="VI319_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863811.1"
                     /translation="MAPRELFNKPVQGLLCPLCLSQSYSLIARRDPKRGILQTVYLCG
                     DCRSYFGDAREFDGKNDSGSDRS"
     gene            1004..1207
                     /locus_tag="VI319_07900"
     CDS             1004..1207
                     /locus_tag="VI319_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863812.1"
                     /translation="MVSRERFAGPVAGIVCPLCLSKSHTVVTRRDPKLGLRQTIYRCE
                     DCKSYFGDPREAADDAPAGPSGE"
     gene            1321..3243
                     /locus_tag="VI319_07905"
     CDS             1321..3243
                     /locus_tag="VI319_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6863813.1"
                     /translation="MSEEPRKDRSEDRFRLLVESVRDYAIFMLDPEGRVATWNAGAQR
                     IKGYAAEEIIGRHFSTFYPPDAIAAQWPQQELEMAKRDGRFEDEGWRLRKDGSRFWAN
                     VVITALYDRSSGELRGFGKVTRDLSERRAHEERVRASEERFRMLVEGVKDYAIFMLDP
                     QGRVVSWNAGAQRIKGYRPEEIIGQHFSVFYPPEALERGWPEYELTVARKEGRLEDEG
                     WRVRRDKSLFWANVVITPLYGEDGVLRGYSKVTRDMTERKHVEALEEGRRQMSEFLAM
                     LGHELRNPLAPIRNAVHVMSRKKLDPTASEWCRDVIDRQVSQLARLVDDLLDVTRITS
                     GKIIVHKMPIDLNLAVERAIESSRPVLDARRHTLKVRLADEPVTVEGDMVRLSQAILN
                     LLNNAAKYTPEGGVVSCAVRRDGANAVVSVRDNGVGIPRDLLPKIFELFVQGDRSLDR
                     SEGGLGVGLTLVDRLVRLHGGTVHAESAGPGMGSEFVVRLPLAEQAAAGSGQDTRKAP
                     EAAESARRVLVVEDNRDAADTLAAAVRSWGHVVQVAYDGTSALEAARDFRPDVALLDI
                     GLPGLNGYQLADRLKSHFGEDELLLIAVSGYAQEEDLERSLNAGIARHFAKPVDLELL
                     RELLARPQTVPNGPGA"
     gene            3318..4253
                     /locus_tag="VI319_07910"
     CDS             3318..4253
                     /locus_tag="VI319_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY6863814.1"
                     /translation="MGVYIGISGWRYPPWRGVFYPPGLAQHRELAWAARVLPTIEING
                     SFYSLQTPQSYAEWYAETPPGFVFGVKGGRFITHVRRLREIEKPLANFFASGVFNLRE
                     KLGPFLWQFPPQFRYDAERFEDFFALLPRDAEQALALARRRDSRMTGRARLAIDANYR
                     LRHAVEIRHESFLDEGFLAQLERHRIALVVADTAGKWPLLERVTSDFMYLRLHGDKAL
                     YASGYGDAALERWAAKIRGWARKRDVYCYFDNDVKVRAPFDARRLIEKLGLADSEAPA
                     RSWTEGRRRLRGAKVETPALQPLRWDFGRKRARVR"
     gene            4283..5254
                     /locus_tag="VI319_07915"
     CDS             4283..5254
                     /locus_tag="VI319_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863815.1"
                     /translation="MRCGARAFVALLFLAFAFPAEAQQWPTRPVRVIVPFALGGATDL
                     VARILGPALAESLGQPFVIENRPGASGNIGVEIAAKAPADGTTLLVGNISTNAINASG
                     FAGRLKFDPVKDLAPVTLVASVPTILVTSPHLPPNTVKELIAYARARPGELNYSNPLG
                     SYSHLDTLDLAARQGIKVVLIPSKGAGSAITSIINGEVHFSFLTMSSVIPHVKAGRMK
                     ALATTSRERVSELPDVPTMAEAGFPGVGSENWNGLFFQAQTPRAIVDRLFAATIAAMG
                     RKPVHDAFVSASLPVTLSKSPEDFREFVDAEVRRWARIVKEHDIHLE"
     gene            5294..6097
                     /locus_tag="VI319_07920"
     CDS             5294..6097
                     /locus_tag="VI319_07920"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY6863816.1"
                     /translation="MEQPLRNQVALVTGASSGIGRGCALALARAGAAVAVDYRSSPDD
                     AKVVVAEIERAGGRAIALQADVSREAEVERLFSDTVARFGTVDILVANAGIQKDAPFK
                     DLSLADWQRVIDVNLTGYFLCARAAVREFLRRGLKPEVSRSAGKIVFVSSVHEIIPWA
                     GHVNYAASKGGISMLMKSLAQEVLEQKIRVNSVAPGAIRTDINRPAWETEKAMRALLN
                     FIPYGRIGEPDDVGRAVAWLASDDADYVAGTTLFIDGGMTLYPEFRHGG"
     gene            6119..6319
                     /locus_tag="VI319_07925"
     CDS             6119..6319
                     /locus_tag="VI319_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863817.1"
                     /translation="MQPKKQPQEQQPKEKQRQPQEKPRPIERDETDALGVKPTKTNRG
                     DSESEVPPEGDEDAGPEEEHQR"
     gene            6503..10669
                     /locus_tag="VI319_07930"
     CDS             6503..10669
                     /locus_tag="VI319_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY6863818.1"
                     /translation="MTQKSKRAVPASAKRAGAPKESLFPIVGVGASAGGLEAFKQFLS
                     ALPRDTGMAFVLVQHLAPTHASMLADILSRETKLPVAEVKDEPAVRPNRVYVIPPDRS
                     MTISGGVLQLQPRKSAPAVHHPVDQFLGSLARDRGHQAIGVILSGTANDGTAGMAEIK
                     AAGGITFAQDDTAEHRGMPRSAIAAGCVDFILPPEGIARKIAELARHPHVYTDHEAAP
                     GSAGGPNPAQGPDFARVLELLHQTTGVNFGQYKTNTLQRRITRRMAVAGVENPAKYLA
                     VLKQDHDELTALYQDILIGVTHFFRNREAFEALRERVLPALFKGRAPDQALRVWVLGC
                     SSGEEAYSLAIVLAEFAESRPLPTPIQLFATDLNGAALEKARAGIYPRTIVRDVSPER
                     IRRFFVETDGHYRVTKQIRDMCVFAQHNVLADPPFSRMDIITCRNLLIYLEPAMQQKI
                     LPLLHYALNPGGVLVLGSSETVGGFRDLFEVEDTKQKIFLKKPGSGRPAPSLAGRDYR
                     GARASAATRPFGTAALDEADLQKEADRLLLTKYAPAGVLVDENLEIMQFRGDTGVCLT
                     PGPGKASHNLLKMAREGLLVPLRAVIARARKTGTPVREEGVRVKANGGQRTVDVEVIP
                     LKPAAGRAPGRAGLLILFQETGQHAVAIERRRAALPRAAGAARRGGPSPDEAEVARLT
                     QELAVTREYLQSVIDQQEVINEELQSANEEVQSANEELQSTNEELETSKEEIQSSNEE
                     LATVNDELQHRNRELGLINSDLVNLLDSVQMPIIMVGHDLRIRRFTPMAERILGLIPA
                     DLGRPMANINLNLSLGDLQPILLGVMDTLATKEVEVQDKHGTWYSLRIRPYRTLDNKI
                     DGAVLTLIDVDTLRRARQYAQSIVATVREPMLVLDGDLRVRTASRSFYQTFQCSPAQV
                     ENRALYDLAERQWNIPELRERMAEVLRRDLPFEDYEMEAEFPTLGRKTLRLNARRLVQ
                     DAGAAPLVLLAIEDVSERTRLASDLEQRVEELAAAHRSKDQFLAVMAHELRNPLAPLH
                     NAVHLLKTKNNAAEALPAALPMMERQIRNLPRLVDDLLDVSRITRGKVELQRERVRLG
                     AIVASAVEAARPVMGANGLHLSVALPPQAVYVDADPTRLEQILGNLLNNAAKFTRRGG
                     HVWLSVATAPGEHGGREAVIRVKDDGIGISEDTLPRVFDLFVQADRSLERAQGGLGIG
                     LTLVRSLVELHGGRVEGRSAGLGKGSEFIVHLPILEEADETSSPQDSEPAQAGSVEGS
                     GGSAPHRVLVVDDNEDSAHSLAELLSAAGHEVKTARDGAQTFETVAAFRPEVILLDIG
                     LPGMDGYEVARRVRRQSGNGRALLIALTGYGRDEDRRLSQEAGFDYHLTKPVDLQKLK
                     TLLAGAPRER"
     gene            complement(10676..>13007)
                     /locus_tag="VI319_07935"
     CDS             complement(10676..>13007)
                     /locus_tag="VI319_07935"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEY6863819.1"
                     /translation="AAARLGFALALNAEQVTAVWPPTGIALAAVLVFGLRAAPGVLLG
                     AFAANASLHEPWLVAAGIGVGNALEAVAGAELLRRLRFDRRLTRVRDVLALLLAAAAS
                     PLIAATLGVGCLVGGNVQPAAAFAALWKLWWIGDALGALIVTPVLIVWGRDSLPRRRG
                     APFEAAALLAALVAVSALAFSEAPAAQLSEYIIFPFLMWGALRFGPAGSAVVALAAYV
                     FEAYATIRGFGPFPATEPGAALVLLQGFMGVAAVTGLVLAAITVQSRSAHESAQRSEQ
                     WLLLALRAARAGAWDWDLVTGKTRRSPGLELVLGLPPGGLGDTQDALRERIAPEDRAR
                     FEAEMRRAIETRSRYSVEVRLAREDGTTAWADTRGEVVTDPSGNPVRMVGIAIDVSRQ
                     KELEQELRRQADQLAEADRRKTEFLAMLSHELRNPLAPIVHAVDLLDSADPKTRLQAQ
                     AVIRRQAAHLARIVGDLLDVSRISRGAIRLEPRRVALADLVATAAEEWRHMMAERGQA
                     LSIDLPPEPVWLEADPTRFAQVISNLVHNSAKFTPHGGRIAIRAETRGKALRLSVRDT
                     GAGIAAEILPQVFEVFVQGAPPFDRAQGGLGLGLALVRRLVELHGGTVEALSDGPGKG
                     SEFVVRMPLAAAPVEAPAPSGEAAPAAALRILVVEDNQDARETLRALLESDGHEVLAL
                     ADGAEALEQAKAFAPDVALLDLGLPGMDGYALARALRATPEGAGIFIAAISGYGQAED
                     RAKSKAAGFDRHLVKPVDPERLRQLLLRQPERSPGA"
BASE COUNT         2063 a         4695 c         4486 g         1763 t
ORIGIN      
        1 tctccggcac caagacctgg atcagcaacg ccccgatcgc cgacctcttt gtggtctggg
       61 ccaaggacga ggccggcgcc atctgcggcc tgctgctgga gcggggctgg gccggactct
      121 ccgcccccga gatccacggc aagatcggcc tgcgcgcctc gatcaccggc gagatcgtga
      181 tggacgacgt gttcgtccct gactcccacc gcctgcccga cgccgccggg atcaaggccc
      241 ccttcgcctg cctcaattcc gcccgctacg gcatcgcgtg gggcgcgctc ggcgccgccg
      301 aggactgctg gcaccgcgcg cggcagtacg tgctcgaccg caagcagttc ggccggccgc
      361 tcgcggcgaa ccagctggtg cagaagaagc tcgccgacat gcagaccgag atcgcgctcg
      421 ggctccaggg ctgcctgcgc ctcggccgcc tcaaggacga gggcgccgac gcgcccgaga
      481 tgacctcgat cctgaagcgc aactcgtgcg gcaagtcgct cgacatcgcg cgactcgcgc
      541 gcgacatgct cggcggcaac ggcatctccg acgagttcgg tgtcgtgcgc cacatgctca
      601 acctcgaggt cgtcaacacc tacgagggca cgcacgacat ccacgccctg atcctcggcc
      661 gcgcgcagac cggcatccag gcgttcagcc cggaatgatg tcgtcctgag cgaagcgaag
      721 gacctgcttt tttgatttag aattcgcccg cgccaccgcc tgcagcgggg gtacgtcgat
      781 cgaaacataa aagcaatggc gcctcgcgaa ctgttcaaca agcccgtcca gggcctgctg
      841 tgcccgctgt gcctgtcgca gagctactcg ctgatcgcca ggcgcgaccc caagcgcggg
      901 attctgcaga ccgtctacct ctgcggcgac tgccgatcct acttcggcga cgcgcgcgag
      961 ttcgacggca agaacgattc cggatcggat agatcctgag cccatggttt cgcgagagcg
     1021 ctttgccggc ccggtcgcgg ggatcgtgtg cccgctgtgc ctctcgaaga gccacaccgt
     1081 ggtgacgcgc cgcgacccga agctcggcct gcggcagacg atctaccgct gcgaggactg
     1141 caagtcctac ttcggcgatc cgcgggaagc cgcggacgac gcgccggccg ggccttcggg
     1201 cgaatagcgc aacccggcgg gaactatccg gagcatcggt tgtcgctgcg acgccgtcat
     1261 tgcggcgctt ccgacacttg ggttagagtt ttcggcttcg ccggagcggc acagggagtc
     1321 atgtccgagg aacctcgaaa agatcgcagc gaagaccgtt tccgcctgct cgtggagagc
     1381 gtgcgggact acgcgatctt catgctcgat cccgagggcc gcgtcgccac ctggaacgcg
     1441 ggcgcgcagc gcatcaaggg ctacgccgcc gaagaaatca tcggccggca tttctcgacg
     1501 ttctatccgc ccgacgccat cgccgcgcag tggccgcagc aggagctcga gatggcgaag
     1561 cgcgacggcc gcttcgagga cgagggctgg cgcctgcgca aggacggctc gcgcttctgg
     1621 gcgaacgtcg tcatcaccgc gctctacgac cgctcgtcgg gcgagctgcg cggcttcggc
     1681 aaggtcacgc gcgacctgag cgagcggcgc gcgcacgagg agcgcgtgcg cgcgagcgag
     1741 gagcgcttcc gcatgctggt cgagggcgtg aaggactacg ccatcttcat gctggatccg
     1801 cagggccgcg tggtgagctg gaacgccggt gcgcagcgca tcaagggcta caggcccgag
     1861 gagatcatcg gacagcactt ctcggtgttc tacccgcccg aggcgctcga gcgcggctgg
     1921 cccgagtacg agctgacggt cgcgcgcaag gagggccgcc tcgaggacga gggctggcgc
     1981 gtgcgcaggg acaagtcgct gttctgggcc aacgtcgtga tcacgccgct gtacggggag
     2041 gacggcgtgc tgcggggcta ctccaaggtg acgcgcgaca tgaccgagcg caagcacgtc
     2101 gaggcgctcg aggaaggccg gcgccagatg agcgagttcc tcgccatgct cggccacgag
     2161 ctgcgcaatc cgctcgcgcc gatccgcaac gccgtgcacg tgatgtcgcg caagaagctc
     2221 gatcccactg cgagcgagtg gtgccgcgac gtgatcgacc ggcaggtctc gcagctcgcg
     2281 cgcctggtcg acgacctgct cgacgtcacg cgcatcacga gcggcaagat catcgtgcac
     2341 aagatgccca tcgacttgaa tctcgcggtc gagcgggcga tcgagtcgag ccggccggtg
     2401 ctcgacgcgc gccggcacac gctcaaggtg cggctcgcgg acgagcccgt caccgtcgaa
     2461 ggcgacatgg tgaggctctc gcaggcgatc ctcaacctgc tgaacaacgc ggccaagtac
     2521 accccggaag gcggcgtcgt ctcgtgcgcc gtgaggcgcg atggcgcgaa cgccgtggtc
     2581 tcggttcgcg acaacggcgt cggcatcccg cgcgacctgc tgccgaagat cttcgagctg
     2641 ttcgtgcagg gcgaccgctc gctcgaccgc tcggaaggcg ggctcggcgt cgggctcacg
     2701 ctcgtcgacc ggctggtgcg cctgcacggg gggacggtcc acgccgagag cgccggaccc
     2761 ggcatgggca gcgagttcgt cgtgcgcctg ccgctcgccg agcaggcggc ggccgggagc
     2821 ggccaggaca cccggaaggc gcccgaagcg gccgagagcg cccgccgcgt gctggtcgtg
     2881 gaggacaacc gcgatgccgc cgacacgctc gcggccgcgg tgcgcagctg gggccacgtg
     2941 gtccaggtcg cgtacgacgg cacgagcgcc ctcgaggccg cgcgcgattt ccggcccgac
     3001 gtggcgctgc tcgacatcgg gctgcccggc ctgaacggat atcagctggc cgaccggctc
     3061 aagtcccatt tcggcgagga cgagctgctg ctcatcgccg tcagcggcta cgcgcaggaa
     3121 gaggacctcg agcgcagcct gaacgcgggc atcgcgcggc atttcgccaa gcccgtggac
     3181 ctcgagctgc tgcgcgagct gctcgcgcga ccgcagaccg tcccgaacgg ccccggcgct
     3241 tagcgcgcgc cttcgatagg ccgaacggcg cggccggcgc gcaacgtcgc gtgaccgcgc
     3301 gcggtcatgc tggcgccatg ggcgtctaca tcggcatctc gggatggcgc tatccgccct
     3361 ggcgcggcgt gttctatccg cccggcctcg cgcagcaccg cgagctcgcc tgggccgcgc
     3421 gcgtgctgcc gaccatcgag atcaacggct cgttctactc gctgcagacg ccgcagagct
     3481 acgccgagtg gtacgccgag accccgcccg gcttcgtgtt cggcgtcaag ggcgggcgct
     3541 tcatcacgca cgtgcgccgg ctgcgcgaga tcgagaagcc gctcgcgaac ttcttcgcct
     3601 ccggcgtatt caacctgcgc gagaagctcg gcccgttcct gtggcagttc ccgccgcagt
     3661 tccgctacga cgcggagcgc ttcgaggact tcttcgcgct gctgccgcgc gacgccgagc
     3721 aggccctcgc gctcgcgcgc cggcgcgact cccgcatgac cggccgcgcg cggctcgcga
     3781 tcgacgcgaa ctaccggctg cgccatgcgg tcgagatccg gcacgagagc tttctcgacg
     3841 agggcttcct cgcgcagctc gagcgccacc gcatcgcgct ggtcgtcgcc gacaccgcgg
     3901 ggaaatggcc cctgctcgag cgcgtcacct ccgacttcat gtacctgcgg ctgcacggcg
     3961 acaaggcgct ctacgcgagc ggctacggcg acgccgcgct cgagcgctgg gcggcgaaga
     4021 tccggggctg ggcgcgcaag cgcgacgtct actgctactt cgacaacgac gtgaaggtgc
     4081 gcgcgccctt cgacgcgcgc cgcctgatcg agaagctcgg cctcgccgat agcgaagcgc
     4141 ccgcgcgcag ctggaccgag ggacgccggc gcctgcgcgg cgcgaaggtc gagacgccgg
     4201 cgcttcagcc gctgcgctgg gacttcgggc gcaagcgcgc gcgagtccgc taaactagcc
     4261 cgctgcagag gaggaatcga gaatgagatg tggcgcccgc gcgttcgtcg cgcttctgtt
     4321 tctcgcgttc gctttccccg cggaagccca gcaatggccg acgcgccccg tgcgcgtgat
     4381 cgtgccgttc gcgctcggcg gcgcgaccga cctggtcgcg cgcatcctcg ggccggcgct
     4441 cgccgagtcg ctgggccagc cgttcgtgat cgagaaccgc ccgggcgcct cgggcaacat
     4501 cggcgtcgag atcgccgcga aggcgcctgc cgacggcacc acgctgctcg tcggcaacat
     4561 ctcgaccaac gccatcaacg cgagcggctt cgccgggcgg ctgaagttcg atcccgtgaa
     4621 ggacctcgcg ccggtgacgc tggtcgcctc ggtgccgacg atcctcgtca ccagccctca
     4681 cctgccgccg aacaccgtca aggagctgat cgcgtacgcg cgcgcgcgcc ccggcgagct
     4741 gaactactcc aacccgctcg gctcgtactc gcacctcgac acgctcgacc tcgcggcccg
     4801 gcagggcatc aaggtggtgc tgatcccctc gaagggcgcg ggctcggcga tcacctcgat
     4861 catcaacggc gaggtgcact tctcctttct caccatgtcg tcggtgatcc cgcacgtcaa
     4921 ggccgggcgc atgaaggcgc tcgcgaccac gtcgcgcgag cgcgtgtccg agctgcccga
     4981 cgtgccgacc atggccgagg cgggattccc cggagtcggc agcgagaact ggaacggcct
     5041 tttcttccag gcgcagacgc cgcgcgcgat cgtcgatcgc ctgttcgccg cgaccatcgc
     5101 cgcgatgggc cgcaagcccg tgcacgacgc gttcgtgagc gcctcgctgc cggtgacgct
     5161 gtcgaagtcg cccgaggact tccgcgagtt cgtcgacgcc gaggtgcgcc gctgggcgcg
     5221 catcgtcaag gagcacgaca tccacctgga gtagccgcgc ggaaccattt ccgggcggac
     5281 ggtcctaccg gagatggaac agccgctccg caaccaggtc gcgctggtga cgggcgcctc
     5341 ctccggcatc ggccggggct gcgcgctcgc gctcgcgcgc gcgggcgcag cggtcgccgt
     5401 cgattaccgg tcgtcgcccg acgatgcgaa ggtcgtggtc gccgagatcg agcgcgcggg
     5461 cggccgcgcg atcgcgctcc aggccgacgt gtcgcgcgag gcggaggtcg aacgcctctt
     5521 ctcggacacg gtcgcgcgct tcggcaccgt cgacatcctc gtcgccaacg cgggcatcca
     5581 gaaggacgcg ccgttcaagg acctctcgct cgccgactgg cagagagtga tcgacgtcaa
     5641 cctcaccggc tatttcctgt gtgcgcgcgc ggcggtgcgc gagttcctgc ggcgcggcct
     5701 gaagcccgag gtctcgcgct cggcgggcaa gatcgtcttc gtctcgtcgg tgcacgagat
     5761 cattccctgg gcgggccacg tcaactacgc ggcctcgaaa ggcggcatct cgatgctgat
     5821 gaagagcctc gcgcaggagg tgctcgagca gaagatccgc gtcaactcgg tcgcgccggg
     5881 cgcgatccgc accgacatca accggcccgc gtgggaaacc gagaaggcga tgcgcgcgct
     5941 gctgaacttc atcccctacg ggcgcatcgg cgagcccgac gacgtcggcc gcgccgtggc
     6001 gtggctcgcc tccgacgacg ccgactacgt cgcgggcacc acgctgttca tcgacggcgg
     6061 catgacgctc taccccgaat tccgccacgg cggctgacga cccaggagac tcatcattat
     6121 gcaaccgaag aagcagccgc aggagcagca accgaaggag aagcagcgcc agccgcagga
     6181 gaaaccgcgg ccgatcgagc gcgacgagac cgacgcgctg ggcgtgaagc cgacgaagac
     6241 gaaccgcgga gacagcgagt ccgaggtccc tcccgaaggc gacgaggacg ccggcccgga
     6301 agaagagcac cagcgctaaa agttctgcac gcgcgccgcg aaagcgccgt cttttgtcgt
     6361 gcccccacgg cacgtcgggg ttcgcattaa gttgtttccg gccaatccga tacgccgcaa
     6421 acgacgtatc tgttcttttc cccggaacgc gtatagagtg atcaacccgc ccccccggga
     6481 gcgcagccgg tccacaatcc tcgtgaccca gaagtccaag cgcgccgttc ccgcatcggc
     6541 caagcgcgcc ggggcgccga aggagtccct gtttcccatc gtcggcgtcg gcgcctccgc
     6601 cggagggctc gaagcgttca agcaattcct gagcgcgctg ccgcgcgaca ccggaatggc
     6661 gttcgtactg gtgcagcacc tcgcgcccac gcacgcgagc atgctcgccg acatcctgtc
     6721 gcgcgagacg aagctccccg tcgccgaggt gaaggacgag ccggcggtgc ggccgaatcg
     6781 cgtctacgtg atcccgcccg accgcagcat gaccatctcc ggcggcgtgc tgcagctgca
     6841 gccgcgcaag agcgcgccgg ccgtgcatca tccggtggac cagttcctgg gctccctcgc
     6901 ccgcgaccgc ggccaccagg ccatcggcgt gattctctcg ggcaccgcga acgacggcac
     6961 cgcgggaatg gcggagatca aggcggccgg gggcatcacc ttcgcgcagg acgacaccgc
     7021 ggagcaccgc ggcatgccgc gcagcgcgat cgccgccggc tgcgtcgatt tcatcctgcc
     7081 cccggagggc atcgcgagaa agatcgcgga gctcgcgcgc catcctcacg tctacaccga
     7141 tcacgaggcc gcgcccggga gcgccggggg accgaacccg gcgcagggcc ccgacttcgc
     7201 ccgcgtgctc gagctgctgc accagacgac cggcgtcaac ttcgggcaat acaagacgaa
     7261 cacgctgcag cggcgcatta cgcggcgcat ggcggtggcg ggcgtggaga atccggccaa
     7321 gtacctcgcc gtcctgaaac aggaccacga cgagctgacg gcgctgtacc aggacatcct
     7381 gatcggcgtc acgcatttct tccgcaaccg cgaggccttc gaggcgctcc gcgagcgcgt
     7441 gctccccgcg ttgttcaagg gtcgcgcgcc cgaccaggcg ctgcgcgtgt gggtgctcgg
     7501 ctgttcgagc ggcgaggagg cctattcgct cgccatcgtg ctcgccgaat tcgccgagag
     7561 ccggcccctg cccacgccga tccagctttt cgccaccgac ctgaacggcg ccgcgctcga
     7621 gaaggcgcgc gccgggatct atccccggac gatcgtccgc gacgtctcgc ccgagcgcat
     7681 acgccgcttc ttcgtcgaga ccgacggcca ctaccgcgtc acgaaacaga tccgcgacat
     7741 gtgcgtgttc gcgcagcaca acgtgctcgc cgacccgccg ttctcgcgca tggacatcat
     7801 cacctgccgc aacctgctca tctacctcga gccggcgatg cagcagaaga tcctgccgct
     7861 gctgcactac gcgctcaacc cgggcggcgt gctcgtcctc ggcagctccg agacggtcgg
     7921 cggcttccgc gacctgttcg aagtcgaaga cacgaagcag aagatctttc tgaagaagcc
     7981 cggctccggg cggcccgcgc ccagcctcgc gggccgcgac taccgcggag cgcgcgcgag
     8041 cgcagcgacg cgcccgttcg gcacggcggc gctcgacgaa gcggacctgc aaaaggaagc
     8101 cgaccgcctg ctgctcacga agtacgcgcc cgccggcgtg ctcgtcgacg agaacctgga
     8161 gatcatgcag ttccgcggcg acaccggcgt gtgcctcacg cccggaccgg gcaaggcgag
     8221 ccacaacctg ctcaagatgg cgcgcgaggg gctgctggtg cctttgcgcg cggtgatcgc
     8281 gcgggcgcgc aagacgggaa ccccggtgcg cgaggaaggc gtgcgggtga aagcgaacgg
     8341 cggccagcgc accgtcgacg tcgaagtcat cccgctcaag ccggcggccg gacgcgcgcc
     8401 gggccgggcc gggttgctga tcctcttcca ggaaaccgga caacatgccg tcgcgatcga
     8461 gcgccggcgc gcagcgctgc cccgcgcggc aggcgccgcg cgcagaggcg ggccgtcccc
     8521 ggacgaggcc gaggtggcgc gcctgacgca ggagctggcg gtgacgcgcg aatacctgca
     8581 gtcggtgatc gaccaacagg aggtgatcaa cgaggagctg cagtcggcga acgaggaggt
     8641 gcagtccgcg aacgaggagc tgcagagcac caacgaggag ctcgagactt cgaaggagga
     8701 gatccagtcg agcaacgagg agctcgccac cgtcaacgac gagctgcagc accgcaaccg
     8761 cgagctcggc ctgatcaaca gcgaccttgt caacctgctc gacagcgtgc agatgccgat
     8821 catcatggtc gggcacgacc tgcgcatccg gcgcttcacg ccgatggccg agcgcatcct
     8881 cgggctcatt cccgccgacc tcggccggcc aatggccaac atcaacctga acctgagcct
     8941 cggggacctg cagccgatcc tgctcggggt catggacacg ctcgcgacca aggaggtcga
     9001 ggtccaggac aagcatggca cctggtactc gctgcgcatc aggccctacc ggacgctcga
     9061 caacaagatc gacggcgccg tgctgacgct gatcgacgtc gatacgctgc gccgcgcgcg
     9121 gcagtacgcg cagagcatcg tagcgacggt gcgcgagccg atgctcgtgc tggacggcga
     9181 cctgcgcgtg cgcaccgcga gccgttcgtt ctaccagacg ttccagtgct cgcctgccca
     9241 ggtcgagaac cgcgccctgt acgatctcgc cgagcggcaa tggaacatcc cggagctgcg
     9301 cgagcgcatg gccgaagtgc ttcggcgcga cctcccgttc gaggactacg agatggaggc
     9361 cgagttcccg acgctggggc gcaagaccct gcgcctgaat gcccgccggc tggtgcagga
     9421 cgccggcgcc gcgccgctcg tgctgctcgc gatcgaggac gtgagcgagc gcacgcggct
     9481 cgcgagcgac ctggagcagc gcgtcgagga gctcgccgcc gcgcatcgca gcaaggacca
     9541 gttcctcgcg gtcatggcgc acgagctgcg caacccgctc gcgccgctgc acaacgccgt
     9601 gcatctgctg aagacgaaga acaatgccgc cgaggcgctg cccgccgcgc tgccgatgat
     9661 ggagcggcag atccgcaacc tcccccgcct cgtcgacgat ctgctcgacg tctcgcgcat
     9721 cacgcgcggc aaggtcgagc tccagcgcga gcgcgtgcga ctgggcgcca tcgtcgcgag
     9781 cgcggtcgag gcggcgcgtc cggtaatggg cgcgaacggg ctgcaccttt ccgtcgcgct
     9841 gccgccgcag gcggtctacg tcgacgcgga tccgactcgg ctcgagcaga tcctcggcaa
     9901 cctgctcaac aacgccgcca agttcacgcg ccgcggcgga cacgtgtggc tctcggtcgc
     9961 gacggcgccc ggcgaacacg gcgggcgcga ggccgtcatc cgggtcaagg acgacggcat
    10021 cggcatcagc gaggacacgc tgccccgcgt gttcgacctg ttcgtgcagg ccgaccgctc
    10081 gctcgagcgc gcacagggcg gcctgggcat cggcctcacg ctggtgcgca gcctggtcga
    10141 gctgcacggc ggccgtgtcg aaggccgcag cgccggactg gggaagggca gcgaattcat
    10201 cgtccacctg cccatcctcg aagaagcgga cgagacatcg tcaccgcagg attccgagcc
    10261 cgcacaggcg ggcagcgtcg agggtagcgg cggatccgcg ccgcaccgcg tcctcgtggt
    10321 cgacgacaac gaagattcgg cgcacagcct cgccgagctg ctgagcgccg cggggcacga
    10381 ggtgaagacg gcgcgcgacg gcgcgcagac cttcgagacc gtcgcggcgt tccggccgga
    10441 ggtgatcctg ctcgacatcg gccttccggg aatggacggc tacgaggtcg cgcggcgcgt
    10501 gcgccggcaa tccggcaacg gccgcgcgct gctgatcgcg ctcaccgggt acggacgcga
    10561 cgaagaccgc aggctgtcgc aggaagccgg gttcgactac cacctgacca agccggtcga
    10621 tctgcagaag ctgaagaccc tgctcgccgg cgcgccgcgc gagcgctgaa tcgcgtcacg
    10681 ccccgggcga gcgctcgggc tggcgcagca gcaattggcg cagccgctcc ggatcgaccg
    10741 gcttgaccag gtgccggtcg aatcccgcgg ccttcgattt cgcccgatcc tccgcctgcc
    10801 cgtagcccga gatcgcggcg atgaagatgc cggcgccctc gggcgtcgcg cgcagcgcgc
    10861 gggcgagcgc gtagccgtcc attcccggaa ggcccaggtc gagcagcgcg acgtcgggcg
    10921 cgaacgcctt cgcctgctcg agtgcctcgg cgccgtcggc gagcgcgagg acctcgtggc
    10981 cgtcgctctc cagcagggcg cgcagcgtct cgcgcgcatc ctgattgtcc tcgacgacca
    11041 ggatgcgcag ggccgccgcg ggcgccgcct cgccggaagg cgccggtgcc tccaccggcg
    11101 ccgccgcgag cggcatgcgc acgacgaact cgctcccctt ccccggcccg tcgctcagcg
    11161 cctcgaccgt tccgccgtgc agctccacca ggcgccgcac cagcgcgagg ccgagtccga
    11221 gaccgccctg cgcgcggtcg aacggcggcg cgccctggac gaagacctcg aagacctgcg
    11281 gcaggatctc ggccgcgatg cccgcgcccg tgtcgcgcac cgacagccgc agcgccttgc
    11341 cgcgagtctc cgcgcggatc gcgatgcggc cgccgtgcgg cgtgaacttc gccgagttgt
    11401 gcacgaggtt cgagatcacc tgcgcgaagc gcgtcgggtc ggcctcgagc cataccggct
    11461 cgggcgggag atcgatcgac agcgcctggc cgcgctcggc catcatgtgc cgccactcct
    11521 ccgccgcggt cgcgacgaga tcggcgagcg ccacccggcg cggctcgagc cggatcgcgc
    11581 cgcggctgat gcgcgacacg tcgagcaggt cgccgacgat gcgcgcgagg tgcgccgcct
    11641 ggcggcggat cacggcctgc gcctgcagcc gcgtcttcgg gtcggcgctg tcgagcaggt
    11701 cgacggcgtg cacgatgggc gcgagcgggt tgcgcagctc gtgcgagagc atcgccagga
    11761 actcggtctt gcgccggtcg gcctcggcga gctggtccgc ctggcggcgc agctcctgct
    11821 cgagctcctt ctggcggctg acgtcgatcg cgatgccgac catgcgcacc gggttgcccg
    11881 acgggtcggt caccacctcg ccgcgcgtgt ccgcccacgc ggtcgtcccg tcctcgcgcg
    11941 cgagccgcac ctcgacgctg tagcgcgagc gcgtctcgat cgcgcggcgc atctcggcct
    12001 cgaagcgcgc gcggtcctcg ggcgcgatgc gctcgcgcag cgcgtcctgc gtgtcgccga
    12061 ggcccccggg cggcagcccc agcaccagct cgaggcccgg cgagcggcgc gtcttgccgg
    12121 tcacgaggtc ccagtcccag gcgcccgcgc gcgcggcgcg cagcgcgagc agcagccatt
    12181 gctcgctccg ctgcgcgctc tcgtgcgcgc tgcggctctg caccgtgatc gcggcgagca
    12241 cgaggccggt cacggccgcc acccccatga agccctgcag cagcacgagc gccgctccgg
    12301 gctccgtcgc cggaaacgga ccgaagccgc ggatcgtggc ataggcctcg aacacgtacg
    12361 cggcgagcgc gaccacggcg ctgcccgcgg gcccgaagcg cagcgcgccc cacatgagaa
    12421 acgggaagat gatgtactcg gagagctgcg ccgcgggcgc ttcgctgaac gcgagcgcgc
    12481 tgaccgcaac cagggcggcg agcagcgcgg ccgcctcgaa cggcgcgccg cgccgcctcg
    12541 gcagcgaatc tcgcccccac acgatcagca ccggcgtgac gatcagcgcg ccgagcgcgt
    12601 cgccgatcca ccagagcttc cacagcgcgg cgaaggcggc ggcgggctgc acgttgccgc
    12661 cgacgaggca gccgacgccg agggtcgcgg cgatcagcgg gctcgccgcc gccgcgagca
    12721 gcagcgcgag cacgtcgcgt acccgcgtca ggcggcgatc gaagcgcagc cggcgcagca
    12781 gctcggcgcc ggcgactgcc tcgagcgcgt tgccgacgcc gatgccggcg gcgaccagcc
    12841 agggctcgtg caggctcgcg ttcgcagcga acgcgccgag gagcacgccg ggcgcggccc
    12901 gcagcccgaa cacgagcacc gccgcgagcg cgatccccgt cggcggccag actgcagtga
    12961 cctgctcggc gttcagcgcg agcgcgaagc ccagccgtgc cgcggcg
//
LOCUS       DATDMW010000180        11648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1299340, whole genome shotgun sequence.
ACCESSION   DATDMW010000180 DATDMW010000000
VERSION     DATDMW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299340"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /gene="selD"
                     /locus_tag="VI319_07940"
     CDS             complement(<1..796)
                     /gene="selD"
                     /locus_tag="VI319_07940"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY6863820.1"
                     /translation="MNAPTDLIRLTQFSHGGGCGCKIAPALLTEILAQAPIRGLPKEL
                     MVGIESGDDAAVYRLNDEQALGATTDFFTPIVDDPYDFGRIAATNALSDVYAMGGRPI
                     FALAIVGMPIEKLPVAAIQKVIAGGESVCAKIGIPIAGGHSIDTLEPIYGLVALGLVH
                     PDKVKRNDRARAGDALVLGKPLGVGILSAALKQGKLFPAGYKQMLAATTALNTPGSRL
                     AEMDGVHAMTDVTGFGLAGHLLGICRGSKLGAEVRFDALPLLPEAAE"
     gene            862..1911
                     /gene="mnmH"
                     /locus_tag="VI319_07945"
     CDS             862..1911
                     /gene="mnmH"
                     /locus_tag="VI319_07945"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY6863821.1"
                     /translation="MKQTGTVTLAQLSEFDEVIDVRSPSEFALDHVPEAVNCPVLDDA
                     ERAEVGTLHKQSSPFDAKKIGAALVSKNIARHIEDRFRDRAREWRPLVYCWRGGKRSG
                     AMAHVLREVGWPAATLEGGYRAYRREVVRQLGELPGRLRFEVICGATGSGKSRLLEAI
                     AARGAQVLDLEGIARHRGSVLGELPDDPQPSQKMFDSMVWDRLRRFDTDRPVYVEAES
                     KKIGQLQVPELLLERMRASACVRLEAPVEARVRFLIEEYGHFLEEPARLKAKLACLRN
                     LHGSEVVARWAAQIDAAQWDQLVTDLLVRHYDPAYQRSTLRNYVRSADPGALELAELS
                     PRSFGEAADGLLANR"
     gene            1921..2298
                     /locus_tag="VI319_07950"
     CDS             1921..2298
                     /locus_tag="VI319_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY6863822.1"
                     /translation="MNPLARLVLVAAALLSGAAGAQTFKCVDAAGKVTYTNGKCSDLG
                     LKNAGEVPDHLNTNPAFRPSSPAPLQPQPSSAPAPAPSKPAAAPAGSAAPGEGDAPDP
                     NRRCFTVKTPTGNVTRCNERPDQ"
     gene            complement(2303..3226)
                     /locus_tag="VI319_07955"
     CDS             complement(2303..3226)
                     /locus_tag="VI319_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY6863823.1"
                     /translation="MATAYITHADCMKHDMGRYHPECPARLSAIEDHLIASGVGATLQ
                     RHEAPEAKISELARVHPLEYIESIREASPERGIVHLDPDTAMCPDTLSAALHAAGAAV
                     HATDLVVGGRVSAAFCAVRPPGHHATRARAMGFCIFNNVAVAVRHALEHHGLERVAVI
                     DFDVHHGNGTEDIFGGDDRVLMASIFQHPFYPYSGTEDPAPNMANVPLAAGSGGAELR
                     AAVEQVWMPALEAHRPQMIFISAGFDAHVEDDMAMLRFTEADYDWVTRRIKEIADRHA
                     RGRIVSVLEGGYALSALGRSVVTHLKVLGGS"
     gene            3313..4233
                     /locus_tag="VI319_07960"
     CDS             3313..4233
                     /locus_tag="VI319_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6863824.1"
                     /translation="MTLASIQRVVQQASQIILGKEGQIRLALACLLARGHLLIEDLPG
                     VGKTTLAHVLAKLLGLNFHRIQFTSDMLPADIIGVSIYDRDSATFKFHPGPVFAQLIL
                     ADEVNRATPKTQSALLEAMEEHQVTAEGETRRLPEPFFVIATQNPSHQVGTFPLPESQ
                     LDRFLMRIELGYPDRDAERALLQGADRRDLIVGVGSCLSPAELSDLQARVKEVHVAAP
                     LLDYIQSLVEHTRRAPEYSTGLSPRAALALLHSSRAWALLEGRDKVIPEDVQAVLPGV
                     AGHRLRPIHDGMRVTSIDKANGLIAAVPIP"
     gene            4242..5210
                     /locus_tag="VI319_07965"
     CDS             4242..5210
                     /locus_tag="VI319_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY6863825.1"
                     /translation="MLDRVLQYSQLYNFYSWVFKRHAPERGVVFLGQRRVYVLPTRHG
                     LTFSLAVLLMLLGSINYVLSLGFVLTFLLAGLGLVSILHTFRNLAHLYVGPGRVDDVF
                     AGEVAGFGLTLESRADFERFALDLTAGGPAITCDVPARRTVYVSVPVRAAKRGWLQLP
                     RVTIETRYPLGLTRAWAYVQPDMRALVYPAPDNAPLPPSAAVPDTGETLTLGAGMDDF
                     AGLRPYQASDSPRHIAWKAVARGGPVLTKTFSGRASSQLWFDWAALPDAMGLEARLSR
                     LTRWVLLAHQEGARFGLRLPGVEIGPAQGEAHKVSCLRELALFEPR"
     gene            5207..7174
                     /locus_tag="VI319_07970"
     CDS             5207..7174
                     /locus_tag="VI319_07970"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY6863826.1"
                     /translation="MIAALRVPAKPGPLPLPLLLWLTAGLAMIAAPHALRLPAWVNGS
                     VAVVLFWRVYLGWRQAELPRRWLLLTLALGQLIAVYFTFRTIFGRDAGVTLLVLLLAL
                     KLMETRAHRDVFVVIFLGYFVALTNFFYSQGIPTGLYTMATVVVITAALVGFNARARP
                     MMDNLGTAGAMLIQAAPVMLLLFLLFPRVQGPLWGMPQDAFGGTTGLSDTMSPGSISQ
                     LSLSDAIAFRAKFHGLRPSNNQLYWRGPVLTSFDGRTWHAAEPRLRYEVQFQAQGRPI
                     EYEITLEPHNRHWLYALDMPAGRPSAARITPEYQVLSLSPVRTRMRYEMISYPSYVVD
                     SGATRDELREDLAIPAGFNPRAQRLAREWRRAAADDAEVVRLALQYFRSNGFLYSLEP
                     PLLGRDSVDEFLFDTREGFCEHYSSSFVFLMRAAGVPARVVTGFQGGAINPVDEYLAV
                     RDSDAHAWAEVWLGASRGWVRVDPTAAVAPARVERGIAAAVPQGSPLPLMIRENLAWL
                     RSLRNSWEALANQWNQWVLGYNPDRQREMLSWLGMEHPSWQSMAMLLFWSIAGVILAT
                     GLWLVFRMRRESAVQRAWLRFCAKLGRAGIMRAAHEGPVDYAARASARLPTRAESIRA
                     IAQLYVDLRYGPRADVERLLELRSLVRGFRA"
     gene            7171..8208
                     /gene="mltB"
                     /locus_tag="VI319_07975"
     CDS             7171..8208
                     /gene="mltB"
                     /locus_tag="VI319_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEY6863827.1"
                     /translation="MTRSRCVLLAAALALAAAQTPARAAESYAKRDDVRQFISVMVQK
                     HGFSKKRLESVFGRVQAQPAVLKSMTLAPESSRAWQSYRSIFVNPQRIDAGVQFWKRH
                     AAALERASAEFGVPEEVILGIIGVETTYGRNMGSYRVIDTLTTLAFDSPNRGEFFRSQ
                     LESFLLFTRESRTDVFRMKGSYAGAIGIPQFMPGSYRQFAVDYDGDGRKDLADSPADA
                     IGSVANFLREHGWRRGEPAAVPATVSSPSAQLLADSGVKPLYRAADLPSFGVEPAGDL
                     ADDTLCSLIELESPAQQSEYWVGLHNFWVLTRYNRAVFYGVAVLELGRAVREAKDART
                     AASEAKPADKP"
     gene            8268..8936
                     /locus_tag="VI319_07980"
     CDS             8268..8936
                     /locus_tag="VI319_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY6863828.1"
                     /translation="MAAFVTVLLCSNFIGAAKQAVITLPGLGVISFGAGVLFFPISYI
                     FGDVLTEVYGYGRDRRVVWAGFAALVFASVMAWAVISLPPARTEFMSGYQAHLEAVFG
                     NAWRIAAGSMIAFWSGSFANSYVLAKMKIWTRGRWLWTRTIGSTLVGEAVDSALFYVI
                     AFAGIWATGDIVKIAATQYVLKSGWEAVMTPVTYRVVGFLKRAEREDYYDRDTDFNPF
                     TLRT"
     gene            9047..9988
                     /locus_tag="VI319_07985"
     CDS             9047..9988
                     /locus_tag="VI319_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863829.1"
                     /translation="MQRRGNEYDVSCKVNTTDSAVVNGEVDRIFHELYPEHSPRQIDQ
                     AFHDIGQLYRGAYPGFHACDTPYHDVQHVMDVTLAMARLIDGYERGRIGAEPVDASMF
                     QLGVIVALFHDCGYVRALDDKLHKNGGELTLTHVSRGAQFLRQYLPKIGMGDVAEIAA
                     SLIHFTGYEMPISHIKVPSLGYKLLGSLLGSADIVAQMSDRCYLEKCRDRLYPEFVHA
                     GIARRRLPDGKEEVVYASGADLVLKTPRFYQGAARRLESDLGGTHVYAERHFGGQDLY
                     LEEVEKNIQFAEDLADRGDADALKRNPPVTQAGHGSR"
     gene            complement(9985..10860)
                     /gene="rpoS"
                     /locus_tag="VI319_07990"
                     /pseudo
     CDS             complement(9985..10860)
                     /gene="rpoS"
                     /locus_tag="VI319_07990"
                     /inference="COORDINATES: protein motif:HMM:NF004207.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
     gene            complement(10908..>11648)
                     /locus_tag="VI319_07995"
     CDS             complement(10908..>11648)
                     /locus_tag="VI319_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY6863830.1"
                     /translation="LRLRAPENIVQVQPASGAGAIEARPLGSDAPAAAPLPTPAPSPA
                     PAARAPGQAPLVTGPKAQKLPWSDENVALLARADAAAAQSQAPALASAPGTSRPDLAK
                     PAPIPAPAPAPQASQGPDDGADRVDWVWPATGKVVAGFSDASNKGVDIAGRVGDPVFA
                     SAGGRVVYSGQGLRGYGKLIIIKHNNTYLSAYAHNSNLLVKEGQNVVRGQKIAEIGST
                     DASQAKLHFEIRRLGKPIDPAKLLPERP"
BASE COUNT         1639 a         4165 c         3996 g         1848 t
ORIGIN      
        1 actcggccgc ctcgggcagc agcggcagcg cgtcgaagcg gacctccgcg ccgagcttcg
       61 agccccggca gatgccgagc aggtggccgg cgaggccgaa gccggtgacg tcggtcatcg
      121 cgtgcacgcc gtccatctcc gcgaggcgcg accccggcgt gttgagcgcc gtcgtcgccg
      181 ccagcatctg cttgtagccc gcggggaaca gcttgccctg cttgagcgcg gccgagagga
      241 tgccgacgcc gagcggcttg ccgagcacca gcgcgtcgcc cgcgcgggcg cggtcgttgc
      301 gcttcacctt gtcggggtgc acgagcccga gcgcgaccag gccgtagatc ggctcgagcg
      361 tgtcgatcga atggccgccc gcgatcggga tgccgatctt cgcgcacacc gactcgccgc
      421 cggcgatcac cttctggatc gccgcgacgg gcagcttctc gatcggcatg ccgacgatcg
      481 caagcgcgaa gatcggccgg ccgcccatcg cgtacacgtc ggacagcgcg ttggtcgcgg
      541 cgatgcggcc gaagtcgtac ggatcgtcga cgatgggcgt gaagaagtcg gtggtcgcac
      601 ccagcgcctg ctcgtcgttc agccggtaca ccgcggcgtc gtcgcccgac tcgatgccga
      661 ccatcagctc cttgggcagg ccgcggatcg gcgcctgcgc gaggatctcg gtgagcagcg
      721 cgggcgcgat cttgcagccg cagccgccgc cgtgagagaa ctgggtgaga cggatcaggt
      781 cggtcggggc attcatcggg tgctcaagta aaatgtcggg ggctcggaat gtcatcctga
      841 gcgaagcgaa ggatctgctt cttgaagcaa accgggacag taacgctagc acagctctcg
      901 gaatttgacg aggtgatcga cgtccgctca ccctcggagt tcgcgctcga ccacgtcccg
      961 gaagcggtca actgccccgt gctcgacgac gccgagcgcg ccgaggtggg cacgctgcac
     1021 aagcagtcgt ccccgttcga cgcgaagaag atcggcgccg cgctcgtctc gaagaacatc
     1081 gcccgccaca tcgaggaccg tttccgcgac cgcgcgcgcg agtggcggcc gctcgtctac
     1141 tgctggcgcg gcggcaagcg ctcgggcgcg atggcgcacg tgctgcgcga ggtcggctgg
     1201 ccggcggcga cgctcgaagg cggctaccgc gcgtaccggc gcgaggtcgt gcgccagctc
     1261 ggcgagcttc ccgggcgcct tcgcttcgag gtgatctgcg gcgcgaccgg cagcggcaag
     1321 agccggctgc tcgaggcgat cgccgcgcgc ggcgcgcagg tgctcgacct cgagggcatc
     1381 gcccgccacc gcggctcggt gctcggcgag ctgcccgacg atccgcagcc gtcgcagaag
     1441 atgttcgaca gcatggtctg ggaccggctg cgtcgcttcg acaccgaccg gcccgtctac
     1501 gtcgaagcgg aaagcaagaa gatcggacag ctgcaggtgc ccgagctcct gctcgagcgc
     1561 atgcgcgcct cggcgtgcgt ccgcctcgag gcgccggtcg aagcgcgcgt gcgcttcctg
     1621 atcgaagagt acgggcattt cctcgaagag cccgcgcgcc tcaaggccaa gctcgcctgc
     1681 ctgcgcaatc tccacgggag cgaggtcgtc gcacgctggg cggcgcaaat cgacgcggcg
     1741 caatgggatc agctcgtcac ggatctgctc gtgaggcact acgatccggc atatcagcgc
     1801 tccacgctca ggaactacgt acggtccgcg gatcccggcg cgctggagct cgccgagctt
     1861 tccccacgga gcttcggcga agccgcggac ggcctcctcg cgaaccggta gactgccgtc
     1921 atgaacccgc tcgcccggct cgtcctcgtc gcggccgcgc tgctctccgg agccgccgga
     1981 gcgcagacgt tcaagtgcgt cgatgcggcc ggcaaggtca cctacaccaa cggcaagtgc
     2041 agcgatctcg gcttgaagaa cgccggcgag gttcccgacc acctgaacac gaacccggct
     2101 ttccgcccgt cgtcgccggc gccgctgcag ccgcagcctt cgtcggcccc ggccccggcg
     2161 ccgtcgaagc ccgcggccgc tcccgcgggc agcgccgcgc ccggcgaagg cgacgccccc
     2221 gatccgaacc gccgctgctt caccgtgaag accccgacgg gaaacgtcac ccgctgcaac
     2281 gagcggccgg atcagtgacc ggctagctcc cgcccagcac cttcaggtgc gtgacgacgc
     2341 tgcggccgag cgccgacagc gcgtacccgc cctcgagcac cgagacgatc cggccacgcg
     2401 cgtgccggtc ggcgatctcc ttgatcctgc gggtcaccca gtcgtagtcg gcctcggtga
     2461 agcgcagcat cgccatgtcg tcctcgacgt gcgcgtcgaa gccggccgag atgaagatca
     2521 tctgcggccg gtgcgcctcg agcgcgggca tccacacctg ctcgaccgcc gcgcgcagct
     2581 ccgcgccgcc cgagcccgcg gcgagcggga cgttcgccat gttgggcgcg ggatcctcgg
     2641 tgccgctgta cggatagaac gggtgctgga agatgctcgc catgagcacc cggtcgtcgc
     2701 cgccgaagat gtcctccgtg ccgttgccgt ggtgcacgtc gaaatcgatg acggcgacgc
     2761 gctccagccc gtggtgctcg agcgcgtgcc gcacggcgac cgcgacgttg ttgaagatgc
     2821 agaagcccat ggcgcgggcg cgggtcgcat ggtggccggg cggccgcacg gcgcagaacg
     2881 cggccgacac ccggccgccg acgaccaggt cggtcgcgtg caccgccgcg cccgccgcgt
     2941 gcagcgccgc cgacagggtg tcgggacaca tcgccgtgtc cggatccagg tgcacgatgc
     3001 cgcgctcggg agacgcctcg cggatggatt cgatgtactc gagcggatgc acccgggcga
     3061 gttcgctgat cttggcttcc ggggcctcgt ggcgctggag cgtggcgccg acgccggagg
     3121 cgatgagatg atcttcgatg gcggagagcc gcgccgggca ctccggatga tacctgccca
     3181 tgtcgtgctt catgcaatcg gcatgggtta tatacgctgt tgccacggtg atgacgaccc
     3241 gatgtgcctt tgttccgcgt cccatcatat cggctttcca gtaaacttgg ggattctccg
     3301 gtccgttcgc gaatgacact tgcgtcgata cagcgcgtcg ttcagcaggc gagccagatc
     3361 atcctcggca aagagggaca gatccggctc gcgctcgcct gcctgctcgc gcgcgggcac
     3421 ctgctgatcg aggatctccc cggcgtcggc aagaccacgc tcgcgcacgt gctggcgaag
     3481 ctgctcgggc tgaacttcca ccgcatccag ttcacgagcg acatgctgcc ggcggacatc
     3541 atcggcgtgt cgatctacga ccgggacagc gcgaccttca agttccatcc cggcccggtg
     3601 ttcgcgcagc tgatcctcgc cgacgaggtc aaccgggcga cgccgaagac gcagtcggcg
     3661 ctgctcgagg cgatggagga gcaccaggtg acggccgaag gcgagacgcg ccgcctgccc
     3721 gagcccttct tcgtgatcgc cacgcagaac ccgtcgcacc aggtcgggac cttcccgctg
     3781 cccgaatcgc agctcgaccg ctttctcatg cgcatcgagc tcggctatcc cgaccgcgac
     3841 gccgagcgcg cgctgctgca gggcgccgac cgccgcgacc tcatcgtcgg cgtcggctcg
     3901 tgcctgtcgc cggccgagct ctcggacctg caggcgcgcg tcaaggaagt gcacgtggcg
     3961 gcgccgctgc tcgactacat ccagtcgctg gtcgagcaca cgcggcgcgc gcccgagtac
     4021 tcgaccggcc tttcgccgcg cgcggcgctc gcgctgctgc actcgtcgcg cgcgtgggcg
     4081 ctgctcgagg gccgcgacaa ggtcatcccg gaggacgtgc aggcggtgct gcccggcgtc
     4141 gccggccacc gcctgcggcc gatccacgac ggcatgcgcg tgaccagcat cgacaaggcg
     4201 aacggcctga tcgcggccgt gcccattccc tgacccggtc catgctcgat cgcgtcctgc
     4261 agtattcgca gctgtacaac ttctacagct gggtgttcaa gcgccacgcg cccgagcgcg
     4321 gcgtggtgtt cctcgggcag cgccgcgtct acgtcctgcc gacgcgccac gggctcacgt
     4381 tctcgctcgc cgtgctgctg atgctgctcg gctcgatcaa ctacgtcctg agcctcggct
     4441 tcgtcctcac cttcctgctc gcgggtctcg gcctggtttc gatcctgcac accttccgca
     4501 atctcgcgca cctgtacgtc ggtccgggac gcgtcgacga cgtgttcgcc ggcgaggtcg
     4561 ccggcttcgg cctcacgctc gagagccgcg ccgacttcga gcgcttcgcg ctcgacctca
     4621 ccgccggcgg gcccgcgatc acctgcgacg tgccggcgcg ccggacggtg tacgtgtcgg
     4681 tgccggtgcg cgcggcgaag cgcggctggc tgcagcttcc ccgcgtcacg atcgagacgc
     4741 gttacccgct cggcctcacg cgcgcctggg cgtacgtcca gcccgatatg cgggcgctcg
     4801 tctatcccgc gccggacaac gccccgctgc cgcccagcgc cgcggtgccg gacacgggag
     4861 agacgctgac gctcggcgcc ggcatggacg atttcgccgg gctgcggccg taccaggcga
     4921 gcgactcgcc gcgtcacatc gcctggaagg ctgtggcgcg cggcggcccg gtgctcacga
     4981 agacgttcag cggacgcgcc tcgtcccagc tctggttcga ctgggccgcg ctccccgacg
     5041 cgatgggcct cgaggcgcgg ctctcccggc tcacgcgctg ggtgctgctc gcgcaccagg
     5101 aaggcgcgcg cttcgggctg aggctgccgg gcgtcgagat cggtcccgcc cagggcgagg
     5161 cgcacaaggt ttcgtgcctg cgcgagctcg cgctgttcga gccgagatga tcgccgcgct
     5221 gcgcgtcccg gccaagcccg gcccgctgcc gctgccgctc ctgctgtggc tcacggccgg
     5281 gctggccatg atcgccgctc cgcacgcgct gcgcctgccc gcctgggtga acggcagcgt
     5341 cgcggtcgtg ctgttctggc gcgtctatct cgggtggcgg caggccgagc tgcccaggcg
     5401 ctggctgctg ctcacgctcg cgctcgggca gctgatcgcg gtgtacttca ccttccgcac
     5461 gatcttcggg cgcgacgccg gcgtgaccct gctggtgctg ctgctcgcgc tgaagctgat
     5521 ggagacgcgc gcccaccgcg acgtcttcgt cgtgatcttc ctcggctact tcgtcgcgct
     5581 gacgaacttc ttctactcgc agggcatacc caccggcctg tatacgatgg ccacggtggt
     5641 cgtgatcacc gccgcgctgg tcggattcaa cgcccgcgcg cggcccatga tggacaatct
     5701 cggcacggcc ggcgcgatgc tcatccaggc ggcgcccgtc atgctgctgc tgttcctgct
     5761 gttcccgcgc gtgcagggcc cgctgtgggg catgccccag gacgcgttcg gcggcacgac
     5821 cgggctttcc gacaccatgt cgccgggctc gatcagccag ctctcgctct ccgacgcgat
     5881 cgcgttccgg gcgaagtttc acggcctgcg cccgtcgaac aaccagctgt actggcgcgg
     5941 ccccgtcctc accagcttcg acggacgcac ctggcacgcg gccgagccgc ggctgcgcta
     6001 cgaggtgcag ttccaggcgc agggacgccc catcgagtac gagatcacgc tcgagccgca
     6061 caaccggcac tggctctacg cgctcgacat gcccgccggg cgcccgtctg cggcgcgcat
     6121 cacgccggaa taccaggtgc tgtcgctctc gccggtccgc acgcgcatgc gctacgagat
     6181 gatctcgtac ccgagctacg tggtcgactc cggcgcgacc cgggacgagc tgcgcgagga
     6241 cctcgcgatc ccggccgggt tcaacccgcg cgcgcagcgc ctcgcgcgcg aatggcgccg
     6301 ggccgcggcc gacgacgcgg aggtcgttcg gctcgcgctg cagtacttcc gctcgaacgg
     6361 cttcctctac agcctcgagc cgccgctgct cggccgcgac agcgtcgacg agttcctctt
     6421 cgacacgcgg gaaggattct gcgagcacta ctcgtcgagc ttcgtcttcc tcatgcgcgc
     6481 ggccggggtc ccggcgcgcg tggtcaccgg cttccaggga ggcgcgatca acccggtgga
     6541 cgagtacctg gccgtgcgcg actcggacgc gcacgcctgg gctgaggtct ggctcggggc
     6601 atcgcgcggc tgggtgcgcg tcgatccgac cgcggcggtc gcgccggcgc gcgtcgagcg
     6661 cggcatcgcg gccgcggtgc cccagggaag ccctttgccg ctcatgatcc gcgagaatct
     6721 cgcctggctg cgctcgctca ggaacagctg ggaggcgctc gcgaaccagt ggaaccagtg
     6781 ggtgctcggc tacaatcccg accggcagcg cgaaatgctg tcgtggctcg ggatggagca
     6841 tccgagctgg cagagcatgg ccatgttgct gttctggagc attgcaggcg tgatcctcgc
     6901 caccgggctt tggctggtct tccgcatgcg ccgcgagagc gcggtgcagc gcgcgtggct
     6961 gcgcttctgc gcgaagctcg gccgcgccgg catcatgcgc gccgcgcacg aaggccccgt
     7021 cgactacgcg gctcgcgcct cggcgcggct gccgacgcgg gccgagtcga tccgcgccat
     7081 cgcgcagctc tatgtcgatc tgcgctacgg gccgcgcgcc gacgtcgagc gcctgctcga
     7141 gctgcgcagc ctggtgcgcg ggttcagggc atgacccgct cgcgctgcgt cctcctcgcc
     7201 gccgcgctgg ccctcgccgc ggcgcagacg cccgcgcgcg cggcggagtc gtacgccaag
     7261 cgcgacgatg tgcgccagtt catctcggtc atggtgcaga agcacggctt ctcgaagaag
     7321 cgcctcgagt cggtcttcgg ccgcgtgcag gcgcagcccg cggtgctgaa gtccatgacg
     7381 ctcgcgcccg agtcgagccg cgcctggcag agctaccgtt cgatcttcgt gaatccgcag
     7441 cgcatcgatg ccggggtgca gttctggaaa cggcacgcgg cggcgctcga gcgcgcgagc
     7501 gccgagttcg gcgtgcccga ggaggtcatc ctcggcatca tcggcgtcga gaccacgtac
     7561 ggccgcaaca tgggcagcta ccgcgtcatc gacacgctta cgacgctcgc cttcgattcg
     7621 ccgaaccgcg gcgagttctt ccgctcgcag ctcgagagct tcctgctttt cacccgcgag
     7681 agccggaccg acgtgttccg catgaagggc tcgtacgccg gcgcgatcgg catcccgcag
     7741 ttcatgccgg gaagctatcg gcagttcgcc gtcgactacg acggcgacgg ccgcaaggac
     7801 ctcgccgaca gccccgcgga cgcgatcggc agcgtcgcca acttcctgcg cgagcacggc
     7861 tggcggcgcg gcgagcccgc ggccgtgccc gcgacggtgt cctcgccctc ggcgcagctg
     7921 ctcgccgact ctggcgtgaa gccgctgtac cgggccgccg acctgccttc gttcggcgtc
     7981 gagcccgcgg gcgatctcgc cgacgacacg ctgtgctcgc tgatcgagct cgagtcgccg
     8041 gcgcagcaat ccgagtactg ggtcggtctg cacaacttct gggtgctcac gcgctacaac
     8101 cgcgcggtgt tctacggcgt cgccgtgctc gagctcggcc gcgcggtgcg cgaggcgaag
     8161 gacgcccgga cggcggcgtc ggaggcgaag ccggcggaca agccgtgagc gaccctgcgg
     8221 cgtcgctcag gacgggcgag cgctcgtacc gctattacga cttcgtgatg gcggccttcg
     8281 tgacggtgct gctgtgctcc aatttcatcg gcgcggcgaa gcaggcggtg atcacgctgc
     8341 cgggcctggg ggtgatctcc ttcggcgcgg gcgtcctgtt cttcccgatc tcgtatatct
     8401 tcggcgacgt cctcaccgag gtctacggct acggccgcga ccggcgcgtc gtctgggcgg
     8461 gattcgccgc gctcgtgttc gcctcggtga tggcgtgggc ggtcatcagc ctgccgcccg
     8521 cgaggacgga attcatgtcg ggctaccagg cgcacctcga ggcggtgttc ggcaacgcgt
     8581 ggcgcatcgc ggccgggtcg atgatcgcct tctggtccgg cagcttcgcc aacagctacg
     8641 tgctggcgaa gatgaagatc tggacccgcg gccgctggct ctggacgcgc accatcggct
     8701 cgaccctggt cggcgaggcg gtggactccg ccctcttcta cgtcatcgcc ttcgccggga
     8761 tctgggcgac cggcgacatc gtcaagattg ccgcaacgca atacgtgctc aagtcgggct
     8821 gggaggcggt gatgacgccc gtgacctacc gcgtcgtcgg cttcctgaag cgcgccgagc
     8881 gcgaggacta ctacgaccgc gacaccgact tcaatccgtt cacgctgcgg acctaggagt
     8941 cgtgaattag tgcacgtccg gggtaaatcg gcccaagggg accggtttct agctgtttac
     9001 cgatcagcgt agaatgcccc acaaacgata agcggagggt tgccccatgc agcgccgagg
     9061 caacgagtac gacgtatcct gcaaagtcaa caccaccgat tccgccgtgg tgaacggcga
     9121 ggtcgaccgg atcttccacg agctctaccc cgagcactcg ccgcgccaga tcgaccaggc
     9181 gttccacgac atcggccagc tctaccgcgg cgcctatccc ggcttccacg cctgcgacac
     9241 cccgtaccac gacgtccagc acgtcatgga cgtgacgctc gccatggcgc ggctgatcga
     9301 cggctacgag cgcggccgca tcggcgccga gccggtggac gcgtccatgt tccagctcgg
     9361 cgtgatcgtc gcgctgttcc acgactgcgg ctatgtgcga gcgctcgatg acaagctgca
     9421 caagaacggc ggcgagctga cgctgaccca cgtctcgcgc ggcgcgcagt tcctgcgcca
     9481 gtacctgccg aagatcggca tgggcgacgt cgcggagatc gccgcctcgc tcatccactt
     9541 caccggctac gagatgccca tctcgcacat caaggtgccg agcctcggct acaagctgct
     9601 cggcagcctg ctcggctcgg ccgacatcgt ggcgcagatg tccgaccgct gctatctcga
     9661 gaaatgccgc gaccgcctgt atcccgagtt cgtgcacgcc ggcatcgcgc ggcgccgcct
     9721 gcccgacggc aaggaggagg tcgtctatgc ctcgggcgcc gatctcgtgc tgaagacgcc
     9781 gcgcttctac cagggggcgg cgcgccggct cgagtccgat ctcggcggga cgcacgtcta
     9841 cgccgagcgc catttcggcg ggcaggacct ctatctcgag gaagtcgaga agaacatcca
     9901 gttcgccgag gacctcgccg accgcggcga cgcggacgcg ctcaagcgca acccgcccgt
     9961 gacgcaggcg gggcacggct cgcgctagag cagcatctcc ttcgacaggc cgcggccgcg
    10021 cagcacgcgc cgcagcgccg cgagcgcttc cagctggatc tggcgcacgc gctcgcgcgt
    10081 cacgcccagc cggaccgcca gctgctccag cgtgcacacc tcctgcccgc tcaagccgaa
    10141 tcgccgctcg atcaccatgc gctgcttgtc gttcagctgt gagagccaga cgcgcaccag
    10201 cgactcgatc tcgctcgcct gcagggcgag ctcggcgggc atcgactgct cgtcggcgat
    10261 ggagtcgccg atcgagagca gcggatcgat gtcgagcggc gcatcgagcg acgccgtgtg
    10321 ctcgctgtgc gcgagcacgt cgcgcacctc gtcggggtcc ttgccaagca gatgcgcgat
    10381 gtcctcgatc gtgggctcgc gctccgagtg cgcccccagg tgccgccgcg cgcgcaggac
    10441 cgtgttcagc tcgcggatga cgtgcaccgg cagccggatc gtgcgcgcct ggttcatgat
    10501 cgcgcgctcg atgttctgcc ggatccacca cgtggcgtag gtcgagaagc ggaacccgcg
    10561 ctcggggtcg aacttgtcga gggcgtgcat gagcccgagg tttccctcct cgatcagatc
    10621 gagcagcggc atgcctcggt tcaggtactg cttggcgacg ttcacgacca gccgcaggtt
    10681 gtgctcgatc atcttctgcc gggcgccgaa gtcgccggcc gcggcgagcc gcgcctggcg
    10741 cagctcctcg accggcgtga acagccggtt cagaccgatg ttgtggaggt agatctgggt
    10801 cgcgtcgctg agcggctgcg cctcgagggc cgcttcgtcc tcggcgattt tgtccggctg
    10861 ctgtccgtcc tcgtcggcgt cgctcgggac gggcgaggac cgccgggtca cggccgctcc
    10921 ggcagcagct tcgcgggatc gatcggcttg ccgaggcggc ggatctcgaa atgcagcttc
    10981 gcctggctcg cgtcggtgct gccgatctcg gcgatcttct ggccgcggac gacgttctgc
    11041 ccttccttca ccaggagatt gctgttgtgc gcgtacgccg agaggtacgt gttgttgtgc
    11101 ttgatgatga tgagcttgcc gtatccgcgc agcccctgcc cgctgtagac cacgcgcccg
    11161 ccggccgagg cgaacaccgg gtcgccgacc ctgccggcga tgtcgacgcc cttgttgctc
    11221 gcatccgaga agccggcgac cactttcccg gtcgcgggcc agacccagtc gacgcggtcg
    11281 gcgccgtcgt cgggtccctg cgatgcctgc ggcgcgggcg cgggcgcggg aatcggcgcc
    11341 ggcttcgcga gatcgggcct ggacgtgccc ggcgcgctcg cgagcgcggg cgcctgggac
    11401 tgcgccgccg cggcgtcggc gcgcgcgagc agcgcgacgt tctcgtccga ccacggcagc
    11461 ttctgcgcct tcggcccggt gacgagcggc gcctgtcccg gagcgcgcgc ggccggtgcc
    11521 ggcgacggcg ccggcgtcgg cagcggcgcg gcggcgggcg cgtcggagcc gagcggtcgc
    11581 gcctcgatcg cgccggcgcc gctcgcgggc tgaacctgca cgatgttctc gggcgcccga
    11641 aggcgcag
//
LOCUS       DATDMW010000181         7745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_56053, whole genome shotgun sequence.
ACCESSION   DATDMW010000181 DATDMW010000000
VERSION     DATDMW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56053"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VI319_08000"
     CDS             <1..222
                     /locus_tag="VI319_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6863831.1"
                     /translation="PYGIVGPRGMEPRVVKTLHDAFKKTLDDPEHQKVLDQLDQELWY
                     RSREDYAKYAREDLQQERAVIERLGLLSK"
     gene            247..684
                     /locus_tag="VI319_08005"
     CDS             247..684
                     /locus_tag="VI319_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6863832.1"
                     /translation="MFNRILLAYNGSREGKSALLACAEIAAFAKSETHLLAVAGMPSS
                     MFLTEGFLPEELLEEEKKRAQEVLNEGISQLQKRGFTVKGHLAVGEPVEEICRLAADI
                     KCDVIVVGHQQKASWASRWWRGSVGKSLLDCSPCSIYITQTRD"
     gene            781..3054
                     /locus_tag="VI319_08010"
     CDS             781..3054
                     /locus_tag="VI319_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6863833.1"
                     /translation="MKGRLEDYRLLTGRGRYTSDWNFENQAYAAFLRSDRAHAEIVSI
                     DAAKALAMPGVLALVTGDDVAREGWRSLPTNLAINDRSGNPLKKPPRPALAQGRVRHV
                     GECVACVVAASPQIAQDALEAIEIEYRDLPSVVDARAALAPGAPQLHESAPGNLVFDY
                     GTGDEPAVAAAFAKAARVVKIALENTRVVGNPLEPRACAATWDAARRHYALYACTQGT
                     AGMRAQLVNAMQIADDSIDVIAEDVGGGFGVRFNLYPEYMAVMLAAKKAARPVKWTGT
                     RSEVFLADEQARDFHCEGELALDATGRFLAMRFSLVANLGAYLAPTGPFVSTQGVSAC
                     LTGVYDVPAASMRTQLAVTNTAPVAAYRGAGRPIMSYILERLVDQAAVELKIDPAELR
                     RRNFVPKDRFPYTTANGGKYDCGDFEGVLDDALAASDWKGFPARREKAKARGRLLGRG
                     ISTYIEATGGGFAPHDQVAVRFGADGGVTLYAVSHSHGQGHETTYAQIVSGVLGVPIE
                     NVRLRNGDPSVRLVGNATGGSRSLLGVGSVMQIAAKEVVEKGRALAAGTLEAAAADIE
                     FRDGEYRIKGTDRAITLTELAKKHPGRLDVQTQHKIGATYPNGCHIAEVEIEPETGEA
                     EIVRYTAVDDAGNVVNPMIVHGQMQGGVTQGAGQVFGELAVYDPDTGQLLTGSFMDYF
                     MPRAGLVPEPVLREHPVPTATNPLGAKGVGEAGVTGSLPALMNAVMDALRQAGVARFD
                     MPATPARVWEAIRAAPH"
     gene            3263..4336
                     /locus_tag="VI319_08015"
     CDS             3263..4336
                     /locus_tag="VI319_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6863834.1"
                     /translation="MGSAAILAVDDDARTLLALRELLGGEGQEVVTAGSGEEALRCAL
                     KQDFAVILLDARMPGVDGFEAARMIRERARSRHTPIIFLTGAYEDVTSMFRGYEAGAV
                     DYLVKPLVPEVLKSKIAVFVELYNKNERLRASEERLRALAAREQSVREEERARIAREI
                     HDELGQALTGLKMDLTWLNGRLPGQKAVAEKVKSMFKLIDDTIQSVRKIATLLRPEVL
                     DQLGLAAAVGWQAREFQMRTGIRCKVTLPAEPVAAGPELSTAAFRIFQELLTNVARHA
                     RATRIDVAMRTDAGRLVLEVEDNGKGISEAELSASKSLGLLGMRERATAFGGGVEFAG
                     GGGKGTRVTVVLPLGPNEKAREN"
     gene            4385..5014
                     /locus_tag="VI319_08020"
     CDS             4385..5014
                     /locus_tag="VI319_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6863835.1"
                     /translation="MRVLIADDHPVVRSGLRQILASERDMVVVGEARNGHEALDLVRK
                     LEWDVAILDFSMPGRSGVDLLKEIKRERPTRPVLVLSMLPEELHATQVFKAGAAGYMN
                     KESACEELTAALRKLANGGKYVSPMLAEKLASELAPDADKPLHETLSDREYRVMWLLA
                     SGRKINHIAKEMFLSPSTISTYRSRILKKLRLSNNAQLVHYAVKNRLVA"
     gene            5186..5878
                     /gene="phoB"
                     /locus_tag="VI319_08025"
     CDS             5186..5878
                     /gene="phoB"
                     /locus_tag="VI319_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY6863836.1"
                     /translation="MNGAILVVEDEPVTQKLITVNLERAGHQVLRAGSVPEAQAAIRE
                     VVPDLILLDWMLPKTTGVMFAKQLRSDRRTKDVPIIMLTARMQETDKVTGLESGADDY
                     IIKPFSPRELLARIKAVMRRCAPQLTDDVVEIAGLKVDPAAHRVTGGGNEIDLGVTEF
                     RMLHFFMTHPERVYSRAQLLDEVWGDHVFVEERTVDVHIRRLRQALAPTGHERLIETV
                     RGTGYRFRKTLV"
     gene            6007..7575
                     /locus_tag="VI319_08030"
     CDS             6007..7575
                     /locus_tag="VI319_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863837.1"
                     /translation="MRPLAVALLTHDYTLARWEADSLRRLESAGVVAVTALIALGSRP
                     RTLERGWLRAQQALEGRMDDPLAAPLDARAAFPRAAAAGEHAGDVVVACGVDPGARGA
                     HAGADTVALSFAGASDDIGLFRAYMHGVPLDLAVTRSSPGGERLLASARPALPERGLL
                     SEIQHEVLRRASGLLEKALRELADELAPMCAGASAIAPPVTLDAVCRYGSRKAARLMR
                     RVLRRLGDSGAGDPDDNWFLAYRTNREAFVCNSERFRADGYRLILPPPGRFYADPCVI
                     EHEGDDHVFFEEWRPDLGRGVISWMRRDGERFAPPEQVLERPYHLSYPFVFRAGGDIF
                     MIPETGRANRIEMYRAVDFPQRWEPAQILVDDIAAADATVLEADGGWWMFATVAEEGS
                     SKCDQLWVFHADAVGGPWKPHVRNPVKIDVRSARPAGSLFRRGGRLIRPTQDCSATYG
                     GALTLCEVTRLTRRDYAEVEIAKLLPGWLPSNLGFHTLSSSRGLEVIDGKMPSGRESR
                     FPALEGALYIEAPA"
BASE COUNT         1195 a         2692 c         2755 g         1103 t
ORIGIN      
        1 ccctacggca tcgtcggccc ccgcggcatg gagccgcgcg tggtgaagac gctgcacgac
       61 gccttcaaga agacgctcga cgacccggag caccagaagg tgctcgacca gctcgaccag
      121 gagctctggt accggtcgcg cgaggattac gcgaagtacg cgcgcgagga cctgcagcag
      181 gagcgcgcgg tgatcgaacg gctcggcctg ctgtcaaaat aacgcccggc cgcgaaggag
      241 gagaccatgt tcaaccggat actgctcgcc tacaacggct cgcgcgaggg caagtcggcg
      301 ctgctcgcct gcgccgagat cgcggcgttc gcgaagtcgg agacgcacct gctcgcggtg
      361 gcggggatgc cctcgtccat gttcctcacc gagggcttcc ttcccgagga gctgctcgag
      421 gaggagaaga agcgcgcgca ggaggtgctg aacgagggca tctcgcagct gcagaagcgc
      481 ggcttcacgg tgaaggggca cctcgcggtc ggcgagccgg tggaggagat ctgccggctc
      541 gcggcggaca tcaagtgcga cgtcatcgtc gtcggccacc agcagaaggc gtcgtgggcc
      601 tcgcgctggt ggcgcggctc ggtgggcaag tcgctgctcg actgctcgcc gtgctcgatc
      661 tacatcacgc agacccgcga ctgagcccgg actgagcgcg ccgggccgcg tggcggccgc
      721 ccgggtcgtt ctggtattct tgggcgttct ccgccatccc gccgcacatc ccctgcaccc
      781 atgaaaggaa gactggaaga ctatcgcctg ctcaccgggc gcgggcgcta cacgtccgac
      841 tggaacttcg aaaaccaggc gtacgccgcg ttcctgcgct cggaccgggc gcacgcggag
      901 atcgtctcca tcgacgcggc caaagcgctg gcgatgcccg gcgtgctcgc gctcgtcacc
      961 ggcgacgacg tcgcccgcga aggctggcgc tcgctgccga ccaacctggc gatcaacgac
     1021 cgctcgggca atcccctgaa gaagccgccg cgcccggcgc tcgcgcaggg acgcgtgcgc
     1081 cacgtcggcg agtgcgtcgc ctgcgtcgtg gccgcgtcgc cgcagatcgc gcaggacgcg
     1141 ctcgaggcga tcgagatcga atatcgcgat cttcccagcg tcgtcgacgc gcgcgccgcg
     1201 ctcgcgccgg gagcgccgca gctgcacgag agcgcgcccg gcaacctcgt cttcgactac
     1261 ggcaccggcg acgagcccgc cgtcgccgcc gcgttcgcga aagcggcgcg cgtcgtgaag
     1321 atcgcgctcg agaacacgcg cgtggtcggc aatccgctcg agccgcgcgc ctgcgcggcc
     1381 acctgggatg cggcgcgccg gcactacgcg ctctacgcct gcacccaggg cacggccggg
     1441 atgcgcgcgc agctcgtcaa cgcgatgcag atcgcggacg acagcatcga cgtgatcgcc
     1501 gaggacgtgg gcggcggctt cggcgtgcgc ttcaatctct atcccgagta catggccgtg
     1561 atgctcgcgg cgaagaaggc cgctcgcccg gtgaagtgga ccggaacgcg ctcggaggtc
     1621 ttcctcgccg acgagcaggc gcgcgacttc cactgcgagg gcgagctcgc gctcgacgcc
     1681 acggggcgct tcctcgcgat gcggttctcg ctggtcgcga atctgggcgc gtacctggcg
     1741 ccgaccggcc ccttcgtcag cacgcagggc gtgtcggcgt gcctcacggg cgtgtacgac
     1801 gtgccggccg cgagcatgcg cacgcagctc gccgtcacga acacggcgcc ggtcgccgcg
     1861 taccggggcg cgggccggcc aatcatgtcc tacatcctcg agcggctggt cgaccaggcc
     1921 gcggtcgagc tgaagatcga ccccgccgag ctgcgccggc gcaacttcgt gccgaaggac
     1981 aggttcccgt atacgaccgc gaacggcggc aagtacgact gcggcgactt cgagggcgtg
     2041 ctcgacgacg cgctcgccgc ctccgactgg aagggctttc ccgcgcggcg cgagaaggcg
     2101 aaggcgcgcg gccggctgct cggtcgcggc atctcgacct acatcgaggc cacgggcggc
     2161 ggcttcgccc cgcacgacca ggtcgcggtg cgcttcggcg ccgacggcgg cgtcacgctc
     2221 tacgccgtgt cgcactcgca cgggcagggc cacgagacga cgtacgcgca gatcgtgtcg
     2281 ggcgtcctcg gcgtgccgat cgagaacgtg cggctccgga acggcgaccc ttcggtgcgc
     2341 ctggtcggca acgcgaccgg cggctcgcgc tcgctgctcg gcgtcggcag cgtcatgcag
     2401 atcgccgcga aggaagtggt cgagaagggc agggcgctcg ccgccgggac gctcgaggcc
     2461 gccgcggccg acatcgagtt ccgcgacggc gagtaccgca tcaagggcac cgaccgcgcg
     2521 atcaccctga ccgagctcgc gaagaagcat ccgggccgcc tcgacgtcca gacccagcac
     2581 aagatcgggg cgacgtatcc gaacggctgc cacatcgccg aggtcgagat cgagcccgag
     2641 accggcgagg ccgagatcgt gcgctacacc gcggtcgacg acgcgggcaa cgtcgtcaac
     2701 cccatgatcg tgcacgggca gatgcagggc ggcgtgacgc agggagcggg gcaggtgttc
     2761 ggcgagctcg ctgtatacga tcccgacacg ggacagctcc tgacgggcag cttcatggat
     2821 tacttcatgc cgcgcgcggg gctggtgccc gagccggtgc tgcgcgagca cccggtgccg
     2881 accgcgacca acccgctcgg cgcgaagggc gtcggcgagg ccggcgtcac gggctcgctt
     2941 cccgccctca tgaacgccgt gatggacgcg ctgcggcagg cgggcgtcgc ccgcttcgac
     3001 atgccggcaa cgccggcgcg cgtgtgggag gcgatccgcg cggcgcctca ttaacgtttc
     3061 tgtagccgac gcgcgacagc gaaaatcgcg cgccctcgac gaaagaattg gcgctcgcgc
     3121 gatggcgagc cgcccgcggc cccgtctatc atcggttcca ggctggcgtc ggttcttccc
     3181 cctcctctct agtgcggacg cccggctcgg gcccggaaac gcagattccg ggcctttttc
     3241 tttggtgcgc taagatggcc gcatgggctc cgccgccatt ctcgccgtcg acgacgacgc
     3301 caggacgctg ctggcgctgc gcgagctgct cggcggggaa ggccaggagg tcgtcaccgc
     3361 cggctcgggc gaggaggcgc tgcgctgcgc gctgaagcag gacttcgccg tgatcctgct
     3421 cgatgcgcgc atgccgggcg tcgacggctt cgaggcggcg cgcatgatcc gcgagcgcgc
     3481 gcgctcgcgc cacacgccga tcatcttcct cacgggcgcc tacgaggacg tcacgtcgat
     3541 gttccgcggc tacgaggcgg gtgcggtcga ctacctcgtc aagccgctcg tccccgaggt
     3601 gctcaagtcc aagatcgcgg tgttcgtcga gctgtacaac aagaacgagc gcctgcgcgc
     3661 ctccgaggag cggctgcgcg cgctcgcggc gcgcgagcag tcggtgcgcg aggaggagcg
     3721 cgcgcgcatc gcgcgcgaga tccacgacga gctcggccag gcgctcaccg ggctgaagat
     3781 ggacctcacc tggctgaacg gccggctgcc cgggcagaag gcggtcgccg agaaggtgaa
     3841 gtcgatgttc aagctgatcg acgacaccat ccagtcggtg cgcaagatcg cgaccctgct
     3901 gcggcccgag gtcctggacc agctcgggct cgcagccgcg gtcggatggc aggctcgcga
     3961 gttccagatg cggaccggca tccgctgcaa ggtgacgctc ccggccgagc ccgtcgcggc
     4021 cggccccgag ctgtcgacgg ccgccttccg catcttccag gagctgctca cgaacgtcgc
     4081 ccgccacgcg cgcgcgaccc ggatcgacgt ggcgatgcgc accgacgccg ggcggctcgt
     4141 cctcgaggtc gaggacaacg gcaagggcat cagcgaggcg gagctgagcg cgtccaagtc
     4201 cctgggcctg ctgggaatgc gcgagcgcgc caccgcgttc gggggcggcg tcgagttcgc
     4261 cggggggggc gggaagggga cgcgggtgac cgtcgtcctg cccctgggcc cgaacgaaaa
     4321 ggcgcgcgaa aactaattcc ttcggaggat gtctatgtga cggcaggcgc acctggagga
     4381 gtccatgaga gtactcatcg ccgacgacca tcccgtcgtc cgcagcgggt tgaggcagat
     4441 cctggcctcg gagcgcgaca tggtggtggt gggcgaggcc cgcaacggcc acgaggcgct
     4501 cgacctggtg cgcaagctcg aatgggacgt cgccatcctg gacttctcca tgccaggacg
     4561 cagcggcgtc gacctcctga aggagatcaa gcgcgagcgg ccgacccgcc cggtgctggt
     4621 gctctccatg ctgcccgagg agctgcacgc gacgcaggtg ttcaaggccg gcgccgccgg
     4681 ctacatgaac aaggagagcg cctgcgagga gctgaccgcg gcgctgcgca agctcgcgaa
     4741 cggcggcaag tacgtgagtc cgatgctcgc cgagaagctc gcctccgagc tcgcgcccga
     4801 cgccgacaag ccgctgcacg agacgctgtc cgaccgcgag taccgcgtca tgtggctgct
     4861 cgcctcgggg cgaaagatca accacatcgc gaaagagatg ttcctttcgc ccagcaccat
     4921 cagcacctac cgctcgcgca tcctgaagaa gctgcgcctc tccaacaacg cgcagctcgt
     4981 ccactacgcg gtcaagaacc gcctcgtcgc ctgattctgc cgatcgtgta gcgggaacgc
     5041 gacgcgccga catcgcgtcg cgacggcgct cgcacgttcg tcgccgcgga gcgaccgtcc
     5101 gagtggattc gatgaactac ttcggggttt ccattgcctt agtctgaaca gagcacgggt
     5161 gggccgggcc gaaggggtga tgagaatgaa tggcgcgata ctggtggtag aagacgaacc
     5221 cgtgactcag aagctcatca cggtcaacct cgagcgcgcc gggcaccagg tgctgcgcgc
     5281 gggcagcgtc cccgaggcgc aggccgcgat ccgcgaagtc gtgccggacc tcatcctgct
     5341 cgactggatg ctgccgaaga cgaccggcgt catgttcgcg aagcaactgc gcagcgaccg
     5401 gcgcaccaag gacgtgccga tcatcatgct caccgcgcgc atgcaggaga ccgacaaggt
     5461 caccggcctg gagtccggcg cggacgacta catcatcaag ccgttctcgc cgcgcgagct
     5521 gctcgcgcgc atcaaggcgg tgatgcgccg ctgcgcgccg cagcttaccg acgacgtggt
     5581 cgagatcgca gggctcaagg tcgatcccgc ggcgcaccgc gtcaccggcg gcggcaacga
     5641 gatcgatctc ggggtgaccg agttccgcat gctgcatttc ttcatgacgc acccggagcg
     5701 cgtctacagc cgcgcgcagc tgctcgacga ggtgtggggc gaccacgtct tcgtcgagga
     5761 gcgcaccgtc gacgtgcaca tccggcggtt gcgccaggcg ctcgcgccga cgggacacga
     5821 gcgtctcatc gagaccgtgc gcggcacggg ctaccgcttc cgcaagacgc tggtctgaaa
     5881 gcctcgtgtc aggctcggtc cggacgctga gccgttcggc gtgtcgccgc gcggcgatgg
     5941 ttgtgtcgat ttgtatccgg acgcgccgag aatggtggcc gtaaacgtta cgcaggccac
     6001 cgcaggatgc gacccctcgc cgtcgcgctg ctcactcacg actacacgct cgcccgctgg
     6061 gaagccgact cgctccggcg gctcgaatcc gccggcgtcg tcgccgtcac cgcgctgatc
     6121 gcgctcggat cgcgcccgcg cacgctcgag cgcggctggc tccgggcgca gcaggcgctc
     6181 gaaggccgca tggacgatcc gctcgctgcg ccgctcgacg cgcgtgcggc gttcccgcgc
     6241 gcggccgccg ccggcgagca tgcgggcgac gtcgtcgtcg cgtgcggcgt ggacccgggc
     6301 gcgcgcggcg cgcacgcggg ggccgacacc gtcgccctgt cgttcgcggg cgcgagcgac
     6361 gacatcgggc ttttccgcgc gtacatgcac ggcgtgccgc tggacctggc ggtcactcgc
     6421 tcgtcgccgg gcggcgagcg ccttctcgcg tcggcgcgcc cggcgctgcc agagcgcggg
     6481 ctcctgtccg agatccagca cgaggtgctg cggcgcgcgt cggggctgct ggagaaggcg
     6541 ctgcgcgagc tcgccgacga gcttgcgccg atgtgcgcgg gagcgagcgc gatcgcgccg
     6601 cccgtcacgc tggacgcggt gtgccggtac ggatcgcgaa aggccgcacg cctgatgcgg
     6661 cgcgtcctga ggcggctcgg ggacagcggc gcgggcgatc ccgacgacaa ctggttcctc
     6721 gcgtaccgca cgaaccgcga ggccttcgtc tgcaacagcg agcgcttccg cgccgacggc
     6781 taccggctga tcctgccgcc gccgggacgc ttctacgccg atccctgcgt gatcgagcac
     6841 gagggcgacg atcacgtgtt cttcgaggaa tggcgcccgg acctcggccg cggcgtgatc
     6901 tcgtggatgc gccgcgacgg cgagcgcttc gcgccgcccg agcaggtgct cgagcgcccg
     6961 tatcacctgt cgtatccgtt cgtgttccgc gcgggcggcg acatcttcat gatccccgag
     7021 accgggcgcg ccaaccgcat cgagatgtac cgcgcggtcg atttcccgca gcgctgggag
     7081 ccggcgcaga tcctggtcga cgacatcgcc gccgcggatg cgacggtgct cgaggccgac
     7141 ggcggatggt ggatgttcgc cacggtcgcc gaggaaggct cgtcgaaatg cgaccagctc
     7201 tgggtcttcc acgccgacgc cgtcggcggt ccgtggaagc cgcacgtgcg caacccggtc
     7261 aagatcgacg tgcgctcggc gcggcccgcg gggtccctgt tccgacgcgg cggcaggctc
     7321 atccgcccca cgcaggactg ctcggcgacc tacggcggcg cgctgaccct gtgcgaggtg
     7381 acgcgtctca cgcgccgcga ttacgcggag gtcgagatcg cgaagctgct cccgggctgg
     7441 ctgccgtcga acctgggatt ccacacgctc tcgagctcgc gcggactcga ggtcatcgac
     7501 ggcaagatgc cctcgggccg ggagtcgcgg ttcccggcgc tcgagggggc gctctacatc
     7561 gaggcgccgg cctgagcctt cgagccggtc actcgacggt gaccgacttc gcgaggttcc
     7621 tgggcttgtc gacgtcgttg ccgcgctcga gcgcggcgtg gtaggcgagc agctgcagcg
     7681 gcaccgagtg caggatgggc gagagcatgc cggcgtggtc gggcatgcgg atgacctgca
     7741 cgccc
//
LOCUS       DATDMW010000182        17232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_57057, whole genome shotgun sequence.
ACCESSION   DATDMW010000182 DATDMW010000000
VERSION     DATDMW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57057"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VI319_08035"
     CDS             complement(<1..856)
                     /locus_tag="VI319_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY6863838.1"
                     /translation="MRNWLLPENIDDILPAEAWRIERLRRRLLELFRGHGYELVIPPM
                     LEYLESLLSGTGHDLDLRTFKLVDQISGRMMGVRADITPQVARIDAHLLNREGVARLC
                     YCGSVLHARPRGLDATREPLQIGAEVYGHAGIESDREVQGLAMQALRASGIREPRIDL
                     GHVAIFRALCRRGGVGAELETELHQALEAKDRPSLESATRRLPKATRAALLALPELYG
                     GREVLARAARALPRHPEIAAALRDLRALTGRTDFQVGIDLADLRGYHYHTGVVFAAYA
                     PGVSNAVAL"
     gene            complement(859..1053)
                     /locus_tag="VI319_08040"
     CDS             complement(859..1053)
                     /locus_tag="VI319_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEY6863839.1"
                     /translation="MTYTTLLMAFALMLILEGILPFLAPAKWREIFRRITEFSDGQIR
                     FFALSSMLVGVLILLLFGGG"
     gene            complement(1096..1974)
                     /gene="hflC"
                     /locus_tag="VI319_08045"
     CDS             complement(1096..1974)
                     /gene="hflC"
                     /locus_tag="VI319_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY6863840.1"
                     /translation="MNNRLVAVLVAVLAVLLLVSLSTFTVDQRQRAIVFQLGEIKEVI
                     NEPGLHFKWPLIQNVRSFDNRILTLDTLETEPFMTSEKKNVLVDSFVKWRIEDLRLYY
                     VAASGDEAQAANRLNGWVKSALFEEFGKRRVHDVISGDRDKIMQIVRERVQDDAKRLG
                     VSIVDVRIKRVELPDEVRDSVYRRMEAERKSAAAQSRSQGFSDGERVRAEADRDREII
                     IAEAYRSAQRVKGEGDARASAIYAKAYSENPEFYAFYRSLDAYRNTFRGKNDVLVIDP
                     SSEFFRYFKNPARSGK"
     gene            complement(1988..3172)
                     /gene="hflK"
                     /locus_tag="VI319_08050"
     CDS             complement(1988..3172)
                     /gene="hflK"
                     /locus_tag="VI319_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY6863841.1"
                     /translation="MSLNDPQWGKRGSGGNEGPPDLDEMWRSFNQKLAGLFGRRRPQD
                     PGGPAGGRNFGGSIGLMIAVVLVAWLASGFYIVVEGQRGVVLTFGKFYEVTGGGPHWR
                     WPYPIQSHEIVDLNQIRTLEIGYRNNVKTRVLRESLMLTDDENIVDAQFSVQYSIRDA
                     REYLFNNRKPDDTVLHAAETALREIVGTSRMDSVLYEERAQIPVLAQKLMQSILDRYQ
                     VGINIVRVTMQNSQPPDQVKDAFADAVRAKQDLERQKNEGQAYYNDVVPKARGTAARL
                     TEEANAYRQRVVADAEGDAARFRQVLVEYNKAPAVTRERLYLDAMQQILSNSSKVVID
                     AKTGGNLLYLPLDKLMQLPAPGASSDGGSSRQPMASEPAPSDGGARSRDTLRSRDREG
                     RP"
     gene            complement(3241..4374)
                     /gene="hflX"
                     /locus_tag="VI319_08055"
     CDS             complement(3241..4374)
                     /gene="hflX"
                     /locus_tag="VI319_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY6863842.1"
                     /translation="MFERPTGGNAAVLVGLDFGAEGYAETLEELGLLAASAGLETRAL
                     VRGRRNRPDPSLFAGSGKVDEIKQALREQSARIAVFNHDLSPAQERNLEKALEARVMD
                     RTTLILNIFAQRAKSHEGKLQVELAQLEHAATRLVRGWTHLERQRGGRGFLGGMGETQ
                     LEVDRRLIGDRVKVLKDKLHKLQRQRSVQRRARSRGNALAVSLVGYTNAGKSTLFNAL
                     THAGTSAANRLFETLDTTTRRLYLPDAGNIVISDTVGFIRDLPHTLIAAFRATLEETI
                     HADLLLHVVDAASDARDVQIDEVNKVLAEIGADGIPQVLVWNKIDRTGAAPGLDRDAY
                     ARIVRVRLSAKTGEGVEFLRQTLTEAAASRTSQPTQHERAVQA"
     gene            complement(4426..4662)
                     /gene="hfq"
                     /locus_tag="VI319_08060"
     CDS             complement(4426..4662)
                     /gene="hfq"
                     /locus_tag="VI319_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY6863843.1"
                     /translation="MSNKGQLLQDPFLNTLRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARAVNLSLDHSDS"
     gene            complement(4752..6083)
                     /gene="der"
                     /locus_tag="VI319_08065"
     CDS             complement(4752..6083)
                     /gene="der"
                     /locus_tag="VI319_08065"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY6863844.1"
                     /translation="MKPTLVLVGRPNVGKSTLFNRLTKSRDALVADVPGLTRDRLYGT
                     GRVGAKPFLVVDTGGLEPGTRDGIFAEMAKQTETAIAEADAIVFLVDAREGLTAQDRR
                     IAEQLRRSGRGILIAVNKTEGLNPEVAVAEFHELGLGAPRAISAAHGEGVGALVDAAL
                     EPFPAEVDAEEGETGVPKVAIVGRPNVGKSTLVNTLVGEERMIAADEPGTTRDAVDVD
                     IEIDGRPYTLIDTAGLRRRGKVFESIEKFSVVKTLQAIDAANVVILVLDARQEISEQD
                     AHIAGFIADRGRALVVAVNKWDGLDDYARDVAKRTIARKLRFLSFARYHYVSGLKGIG
                     IRPLFASVDAAYRAAMVRLPTPKLTRALIAAVAKQAPPRAGYSRPKLRYAHQGGRNPP
                     TIIVHGTALDAVPDSYRRYLEGEFRAVFALEGTPLRIEFRTGRNPYAPKRV"
     gene            complement(6080..7213)
                     /gene="bamB"
                     /locus_tag="VI319_08070"
     CDS             complement(6080..7213)
                     /gene="bamB"
                     /locus_tag="VI319_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEY6863845.1"
                     /translation="MRALSRGAALAASIALAGCFGSSGPKMAELQDIKTTLSVRLAWE
                     ARVGDAKEAVLAPVVAGGYVYAAAHDGTVVSLDAARGRERWRADAGEALSGGVGTDGV
                     IVAVGSEEGQVVALDAATGKARWRSRVSSEVLSPPVVTGDLVLVRSGDSRLFALDAKD
                     GTRRWLYQRATAPLSVRATEGMAVSRGIVYAGFSGGKLMALSLANGAPRWEATVAVPK
                     GSTELERVSDVVGIPAVGERDVCAAAYQGRVGCFDLASANPLWTRELSSSAGVALDPG
                     FVFVSDEKGSVHALDRSSGTSVWKQDKLFLRRLTAPLPLGRSVAVADVEGYVHLLARD
                     TGGFVARVETDGTPVSAYLVRIEGGFLAQTRGGALYAFTVADK"
     gene            complement(7200..7841)
                     /locus_tag="VI319_08075"
     CDS             complement(7200..7841)
                     /locus_tag="VI319_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6863846.1"
                     /translation="MSAYDLEEQEQLAELKAWWKTHGNAVLTAAIVVLLAVAAWNGWT
                     WYQRTQAAQAGGLYDTLQKAARANDLKATRDSAGAILENYPRTTYAPLAALTSAKVHF
                     QAGDLKTARAQLAWVVDHARNAQLRDIARLRLASVLLDDGAPDEALKVLDAKPDPAFE
                     ALFAAERGDVLVLQKKPAEARTAYRSALDKASDRDGALRESLRLKLDALGEGS"
     gene            complement(7838..9139)
                     /gene="hisS"
                     /locus_tag="VI319_08080"
     CDS             complement(7838..9139)
                     /gene="hisS"
                     /locus_tag="VI319_08080"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY6863847.1"
                     /translation="MTQPIQAVRGMNDILPDETERWEALEELLRAWLRSYGYRNIRTP
                     LLESTQLFRRAIGEATDIVEKEMYSFVDELNGEALTLRPEATASTVRAAIEHSLLYNG
                     PQRLYYLGPMYRHERPQKGRYRQFHQVGAEALGFAGPDVDAELLLMCRRLWDDLGLEG
                     VRLELNSLGSAAERAAYRAGLVAYLKKREGELDADSRRRLDSNPLRVLDSKNPAMQPI
                     IAEAPRLIDTLGDASLKHFDGVQAILKDAGQPYEINARLVRGLDYYNLTVFEWVSDRL
                     GAQGTVCAGGRYDGLFEQLGGKPTPACGFAMGIERLLAVAGEGAKGEAPDVYLVNQGE
                     DAARFAFRVAEALRDAGFGVLQHAGGGSFKSQMKKADASGAWVAVIVGDDEARAGAAG
                     VKALREEREQSRVPLAGVADAVAALLLDGGEDKETRKSNGK"
     gene            complement(9150..10433)
                     /gene="ispG"
                     /locus_tag="VI319_08085"
                     /gene_synonym="gcpE"
     CDS             complement(9150..10433)
                     /gene="ispG"
                     /locus_tag="VI319_08085"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY6863848.1"
                     /translation="MSQSPGSARRKTRQVRIGRVAIGGGEPIVVQSMTNTDTADAQAT
                     VRQVLDLARAGSELVRVTVNTAEAAAAVAGIRERLDAAGCDVPLVGDFHFNGHKLLQE
                     HPDCARALDKYRINPGNVGRGSKRDEQFATLIEIACREAKPVRIGVNWGSLDQELLAR
                     LMDENAKRAQPLEADLVMREALVASALESAARAEALGLPGDRIVLSCKVSGVQDLIAV
                     YRELASRCDYPLHLGLTEAGMGTKGVVASSAALAVLLQEGIGDTIRISLTPEPGGDRT
                     REVVVAQELLQTMGLRSFAPMVIACPGCGRTTSTTFQELADRIQRFLREKMPEWRARY
                     PGVADMHVAVMGCVVNGPGESKQANIGISLPGSGERPVAPVYVDGQKTVTLKGERIAE
                     EFQALVERYVEQTYGGGSPGRDAPPAKTIPIKTAV"
     gene            complement(10439..11422)
                     /locus_tag="VI319_08090"
     CDS             complement(10439..11422)
                     /locus_tag="VI319_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEY6863849.1"
                     /translation="MNDAVTADVATGRLLARLRAERKLTVADVAQRLKYAVRQIEALE
                     AEEFSRLPGTTFVRGMVRGYAKLLEIDAEPLLKSLEARQIPGAATVDLRAKRVPFPDG
                     TRRRRGTRLYAVMSALVLVAVGGVLYEWHSGGLPLPKRSAPAELADADAAQVPAAPEA
                     APAPVTRVQAEKPAAAPTREIPKAATSLAQNGAQPARPAGTAASAAPTPKIASAAAPA
                     PSTANAPPTPAKTAGSAAPAPVAKDVKSIPGQGRIVMEFDDDAWVEINDGDGKLLMAQ
                     LNHAGSRRVVSGHPPFSVVIGNAASVHVVYNDRPLDLVPYVKVEVARFTLP"
     gene            complement(11419..12204)
                     /gene="pilW"
                     /locus_tag="VI319_08095"
     CDS             complement(11419..12204)
                     /gene="pilW"
                     /locus_tag="VI319_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY6863850.1"
                     /translation="MSVARSLALALLVAGPLSGCAGSGSGSGDDTGPVIGDVAPPRER
                     ARAHTELAAAYLQRGNLGVALEESRRALRADSGYAPAYNVLGLVQMDLRENSEAERAF
                     ERALRIDPNDADTNHNYGWFLCRTGREDESVRYFMNAVRNPLYARPQKTYAVAAGCVQ
                     KKNPKDALDFYDRALRLDPTYPPALIGLAQLRYRRGELRDARALVQRFNKAVDPTAES
                     LWLALRIERKLGDRVAEGSYATQLRRRFAGSAEYQELQKGRYE"
     gene            complement(12201..13289)
                     /gene="rlmN"
                     /locus_tag="VI319_08100"
     CDS             complement(12201..13289)
                     /gene="rlmN"
                     /locus_tag="VI319_08100"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY6863851.1"
                     /translation="MSVNLLDLDGAALERFVAGLGEKPFRARQLKRWLHRAGEPDFAR
                     MSDLAKGFREKLAREAVVRAPRIVSDTTAEDGTRKWLLDIGADNAIETVFIPEAARGT
                     LCVSTQAGCTLACGFCSTGRQGFNRNLGVGEIVGQLWLASRSVDGAAGVAGERRITNV
                     VMMGMGEPLLNFEPTVAALGLMLDDDAYGLSRRRVTVSTSGIVPMIDRLRDECPVALA
                     VSLHAPNDVLRDRLMPINRKYPLAELLDACNRYLERAPRDFITFEYVMLRGVNDGDEH
                     ARELVGIARRVPCKINLIPFNPFPDSGFERSPRETIRRFQDTLNAAGVVTTTRKTRGD
                     EIDAACGQLAGQIQDRTRKTVRLARLAS"
     gene            complement(13286..13756)
                     /gene="ndk"
                     /locus_tag="VI319_08105"
     CDS             complement(13286..13756)
                     /gene="ndk"
                     /locus_tag="VI319_08105"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY6863852.1"
                     /translation="MAIERTLSIVKPDAVAKNVIGQIYSRFEKAGLRVVAARMMHLSE
                     REAEGFYAVHRERPFFRDLVQFMISGPIMVQVLEGENAVARNRELMGATDPRKADKGT
                     IRADFAESIDANAVHGSDSAENARIEIAYFFPACEVFPRRVANARKPEPERATS"
     gene            14103..14825
                     /locus_tag="VI319_08110"
     CDS             14103..14825
                     /locus_tag="VI319_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6863853.1"
                     /translation="MRVLIVEDDKALADGLTRTLKQSGYAVDHAATGELALRACAEEH
                     YDLIVLDISLPGIDGFEVLRQLRRNHRAGSILILTAHDSEGDRVRGLDLGADDYVTKP
                     FSLPELEARVRALIRRSQAVRSPKLRFGGLTVDTVARRASIGAEPLDLTPREWAVLEY
                     LLMRIGQVVSKEHMLQAMCSWDDSLTHNAVEVYISRLRSKLQHAGIRIRTVRGFGYMV
                     EDPDTAEAGTPVPDTADRPGMR"
     gene            14828..16261
                     /locus_tag="VI319_08115"
     CDS             14828..16261
                     /locus_tag="VI319_08115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY6863854.1"
                     /translation="MPRSRSLRQRLTYWLFGPLLLLVGAGTFASYRIALNAANDAYDS
                     ALLDPALAIASHVRRAGGRLELDLPNVALEALRYDAQDRVFLQVRGPGGELIAGSPQL
                     PAPPERVGPDRNIFYDAKLDTERVRVAALGVRAETGIVLVQVAETFVKRDKLVLELLI
                     ASTLPQVMIAIAAVALFWFGIGRGLTPLDRLSDEIAARSPRDLRAVHEEGQPLEVKPL
                     VVALNRLLSRLSAAIESQQRFIANAAHQLRTPLAGLKTHAELARRQPSTVELRALLDM
                     IAGETERTSHLINQLLTLARAEPQEAHAGAGQPLDLHEQVGRDVREWVQRAVDKNIDL
                     GFELEDAWTLGQPLLVRELVGNLLDNALAYTQPGGAVTVRTRESGAEATVEVEDNGPG
                     IPESEREKVFERFYRMPATGGEGCGLGLSIVAEIAERHGARVELGAPARGRGTLVRVL
                     FPRLAREALAANDARGGKALASRTAPS"
     gene            complement(16239..>17232)
                     /gene="tilS"
                     /locus_tag="VI319_08120"
     CDS             complement(16239..>17232)
                     /gene="tilS"
                     /locus_tag="VI319_08120"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY6863855.1"
                     /translation="RVQADAIALAQHRDDQAETLLLQLARGTGVRGMAGMPGERLLEG
                     SSIRILRPLLDVSRAEIEACARERGLAWVEDESNDDTARRRNFVRHEVLPRVERQFPA
                     ARAAIARAARHLREAGDLADALAREDLERIGGMPLDAVALLTLGEARAKNLLRHCCER
                     DGIGAPGARRLGELLRQLSAARQDSAVSVRVSGWCFRRYRGRVYFEPERAPSEVCEAW
                     NGESALPLLALGGVLRFKPEEGKGLSVDKLRSAAVTVRARRGGERLRPDCRRPRRTLK
                     NLLQERRVPPWRRERLPLLYCGEDLVSVPGVGDECAYHADAGEPGVIVSWEPFD"
BASE COUNT         2438 a         6113 c         5962 g         2719 t
ORIGIN      
        1 cgagggcaac cgcgttcgac acgcccgggg cgtacgcggc gaacaccacg cccgtgtggt
       61 aatggtatcc gcgcaggtcg gcgagatcga ttccgacctg gaagtcggtc cttccggtga
      121 gcgcgcgcag gtcgcgcagc gcagccgcga tctcgggatg acgcggcagc gcgcgcgccg
      181 cgcgcgcgag cacctcgcgc ccgccgtaga gctcgggcag ggccagcagc gccgcgcgcg
      241 tcgccttcgg caggcggcgc gtcgccgatt cgagcgaggg ccggtccttc gcctccagcg
      301 cctggtgcag ctcggtctcg agctcggcgc cgacgccgcc gcgccggcac agcgcgcgga
      361 agatggcgac gtgcccgagg tcgatgcgcg gctcgcggat gccgctcgcc cgcagcgcct
      421 gcatcgccag gccctgcacc tcgcggtcgc tctcgatccc cgcgtgcccg tagacctcgg
      481 cgccgatctg caggggctcg cgcgtcgcat cgaggccgcg cggtcgcgcg tgcagcacgc
      541 ttccgcaata gcacaatcgc gcgacgccct cgcggttcag cagatgcgcg tcgatgcgcg
      601 cgacctgcgg cgtaatgtcg gcgcgcacgc ccatcatgcg gcctgagatc tggtcgacca
      661 gcttgaacgt gcgcaggtcg aggtcgtgcc ccgtgcccga gagcagcgac tcgaggtact
      721 cgagcatggg cgggatcacg agctcgtagc cgtgcccgcg gaacagctcg agcaggcgcc
      781 ggcgcagccg ctcgatgcgc cacgcctcgg cgggcaggat gtcgtcgatg ttctcgggca
      841 gcaaccagtt gcgcatggtc atccgccgcc gaacagcagc aggatcagca cgccgacgag
      901 catggagctc agcgcaaaga agcggatctg cccgtccgag aactcggtga tgcggcggaa
      961 gatctcccgc cacttggcgg gcgcgagaaa gggcaggatg ccctcgagaa tcagcatcag
     1021 cgcgaacgcc atcagcaggg tggtgtacgt catccctcgc ctctccctat gctcgcgctg
     1081 gcgtcagcgg gtgagctact tcccgctccg cgcgggattc ttgaagtatc ggaagaactc
     1141 ggagctggga tcgatcacga gcacgtcgtt cttgcccctg aaagtgttgc gataggcgtc
     1201 gaggctgcgg tagaaggcgt agaactcggg gttctcgctg tacgccttgg cgtagatcgc
     1261 cgaggcgcgc gcgtcgccct cgcccttcac ccgctgcgcg ctgcggtacg cctccgcgat
     1321 gatgatctcg cggtcgcggt cggcctcggc gcgcacgcgc tcgccgtcgg aaaagccctg
     1381 cgagcggctc tgcgcggcgg cgctcttgcg ctcggcttcc atgcgccggt acaccgagtc
     1441 gcgcacctcg tcggggagct cgacacgctt gatgcgcaca tcgacgatgc tgacgcccag
     1501 gcgcttggcg tcgtcctgga cgcgctcgcg cacgatctgc atgatcttgt cgcggtcgcc
     1561 ggagatgacg tcgtgcacgc ggcgcttgcc gaattcctcg aacagcgccg acttcaccca
     1621 gccgttcagg cggttcgcgg cctgcgcctc gtcgccggat gccgccacgt agtacagccg
     1681 caggtcctcg atgcgccatt tcacgaagga atcgaccaga acgttcttct tctccgaggt
     1741 catgaacggc tcggtctcca gcgtgtcgag cgtcaggatg cggttgtcga aggacctgac
     1801 gttctggatc agaggccatt tgaagtgcag gcccggctcg ttgatgacct ccttgatctc
     1861 gcccagctgg aacacgatcg cgcgctggcg ctggtccacg gtgaacgtgg acaggctcac
     1921 cagcaagagc acggcaagga cggcgacgag cacggcgacg aggcggttgt tcatgcggct
     1981 ctcaccttca agggcgccct tcgcggtcgc ggctgcgcag cgtgtcgcgc gagcgcgcgc
     2041 cgccgtctga gggcgcgggc tctgaagcca tcggctggcg cgagcttccg ccgtcggacg
     2101 aggcgccggg cgcgggcagc tgcatcagct tgtcgagcgg caggtacagc aggttgccgc
     2161 cggtcttcgc gtcgatcacc accttgctcg agttcgacag gatctgctgc atcgcgtcca
     2221 ggtacaggcg ctcgcgcgtg accgcgggcg ccttgttgta ctcgaccagg acctggcgga
     2281 agcgcgcggc gtcgccctcg gcatcggcaa ccacgcgctg gcggtaggcg ttcgcctcct
     2341 cggtgagacg cgcggccgtg ccgcgcgcct tgggcacgac gtcgttgtag taggcctggc
     2401 cctcgttctt ctggcgctcg aggtcctgct tggcgcgcac cgcgtcggcg aaggcatcct
     2461 tcacctggtc gggcggctgc gagttctgca tcgtgacgcg cacgatgttg atgcccacct
     2521 ggtagcggtc gaggatcgac tgcatcagct tctgcgcgag caccgggatt tgcgcgcgtt
     2581 cctcgtacag caccgagtcc atccggctcg tgcccacgat ctcgcgcagc gccgtctcgg
     2641 ccgcgtgcag cacggtgtcg tcgggcttgc ggttgttgaa gaggtactcg cgcgcgtccc
     2701 ggatgctgta ctggaccgag aactgcgcgt cgacgatgtt ctcgtcgtcg gtcagcatca
     2761 gcgactcgcg cagcacgcgc gtcttgacgt tgttgcggta gccgatctcg agcgtgcgga
     2821 tctggttgag gtcgacgatc tcgtggctct ggatgggata cggccagcgc cagtgcggcc
     2881 cgccgccggt gacttcgtag aacttcccga acgtgagcac gacgccgcgc tggccttcaa
     2941 cgacgatgta gaagccgctc gcgagccagg cgaccagcac cacggcgatc atgagcccga
     3001 tgctgccgcc gaaattgcgg ccgcccgcgg gtccgcccgg gtcctgcggg cgacgcctgc
     3061 cgaacaggcc ggcgagcttc tggttgaagc tgcgccacat ctcgtcgagg tcgggcgggc
     3121 cttcgttgcc tccgctgcct cgcttgcccc attgcgggtc gttcaatgac ataccggacc
     3181 gggtcctcgg ggaatctttc gaatccgccc gtctcgcaag cggctccctt tgcttccagg
     3241 ctacgcctgg accgctcttt cgtgctgagt cggctgagag gttctcgagg ccgccgcctc
     3301 ggtcagggtc tggcgcagga actcgacgcc ttcgcccgtc ttcgcgctga gacgaacgcg
     3361 gacgattcta gcataggcgt ctcgatccaa gcccggagcc gcgcccgtgc ggtcgatctt
     3421 gttccagacg agcacctgcg ggatgccgtc ggcgccgatc tcggcgagca ccttgttgac
     3481 ctcgtcgatc tgcacgtcgc gcgcgtcgct ggccgcgtcg accacgtgca gcagcaggtc
     3541 cgcgtggatc gtttcctcga gcgtcgcgcg gaacgccgcg atcagggtgt gcggcaggtc
     3601 gcggatgaag ccgaccgtgt ccgagatcac gatgttgccc gcgtcgggaa gatacaggcg
     3661 gcgtgtcgtc gtatcgagcg tctcgaacaa tcggttcgcg gccgaggtgc cggcgtgcgt
     3721 cagcgcgttg aacagcgtcg acttgcccgc gttggtatag ccgaccagcg acacggcgag
     3781 ggcgtttccg cgcgagcgag cgcgccgctg cacgctgcgc tggcgctgca gcttgtgcag
     3841 cttgtccttg agcaccttga cgcggtcgcc gatcagccgc cggtcgacct cgagctgcgt
     3901 ctcgcccatg ccgccgagga agccgcggcc cccgcgctgg cgctcgaggt gcgtccagcc
     3961 ccgcaccagg cgcgtcgccg cgtgctcgag ctgcgccagc tcgacctgga gcttgccctc
     4021 gtggcttttc gcgcgctgcg cgaagatgtt caggatcagc gtcgtgcggt ccatcacccg
     4081 ggcctcgagc gccttctcga gattgcgctc ctgggccggc gaaaggtcgt gattgaagac
     4141 cgcgatgcgg gcgctctgct cgcgcagcgc ctgcttgatc tcgtccacct tgccgcttcc
     4201 cgcgaacagc gacggatccg gacggttgcg ccggccgcgc acgagcgcgc gcgtctccag
     4261 accggcgctc gcggcgagga ggcccagctc ctcgagcgtc tcggcatagc cctcggcccc
     4321 gaaatcgagg cctacgagca cggccgcgtt gccgcccgtc ggacgttcga acatcagatg
     4381 gggttcaggc gagcggtccc tgtccgctgc gccgcaggac ggacatcaac tgtcggagtg
     4441 atccagcgag agattgaccg cacgcgccgg caccacggtc gagatcgcgt gcttgtagac
     4501 catctgcgtg acggtgttct tgagcaggac tacgtactgg tcgaaggact cgatctggcc
     4561 ctgcagcttg atgccgttga cgaggtagat ggaaaccgga acgtgctcct tgcgcaaggt
     4621 gttcagaaac gggtcctgca gtaattgtcc tttgttgctc atgggttacc cccgagtccg
     4681 ttcttttttg gaatgctact gtaattgatt tttaaggcaa aagccaacgc gctcgcaatc
     4741 gcaagcaccc gttagacccg cttcggcgcg taggggttcc ggccggtgcg gaattcgatc
     4801 ctgagcggcg tgccctcgag cgcgaacacg gcgcggaact cgccttcgag gtagcgccgg
     4861 tacgagtccg gtacggcgtc gagcgcggtg ccgtgcacga tgatggtcgg cggattgcgc
     4921 ccgccctggt gggcgtagcg aagcttcggc cgcgagtacc cggcgcgagg cggcgcctgc
     4981 ttggcgactg ccgcgatcag cgcgcgcgtg agcttcggcg tcggcaggcg gaccatcgcc
     5041 gcgcggtacg cggcgtcgac ggacgcgaac agcggccgga tgccgattcc cttgagcccg
     5101 gagacgtagt gatagcgcgc gaacgaaagg aaccggagct tgcgcgcgat ggtgcgcttc
     5161 gccacgtcgc gcgcgtagtc gtccaaccca tcccatttgt tcacggcgac caccagcgct
     5221 cgcccgcgat cggcgatgaa cccggcgatg tgcgcgtcct gctccgagat ctcctggcgc
     5281 gcgtcgagca cgaggatgac gacgttggcg gcgtcgatcg cctgcagcgt cttgacgacc
     5341 gagaacttct cgatgctctc gaagaccttg ccgcggcggc gcagcccggc cgtgtcgatg
     5401 agggtgtagg ggcgcccgtc gatctcgata tcgacgtcga ccgcgtcgcg cgtcgtgccg
     5461 ggctcgtccg cggcgatcat gcgctcctcg ccgacgagag tgttcaccaa ggtcgacttg
     5521 ccgacgttgg gacggcccac gatcgccacc ttcgggacgc cggtctcccc ctcttcggcg
     5581 tcgacctcgg cgggaaacgg ctcgagcgcc gcgtccacga gcgcgccaac cccctcgccg
     5641 tgcgcggcgg agatcgcccg cggggcgccg aggccgagct cgtggaactc ggcgaccgcg
     5701 acttccgggt tcaggccctc ggtcttgttc accgcgatca ggatcccccg cccggaacgg
     5761 cgcagctgct cggcgatgcg ccggtcctgc gcggtcaggc cctcgcgcgc atcgaccagg
     5821 aagacgatcg cgtccgcctc ggcgatcgcg gtctcggtct gcttcgccat ctcggcgaag
     5881 atgccgtcgc gcgtgcccgg ctccaggcct cccgtgtcca ccacgagaaa cggcttggcg
     5941 ccgacgcgcc ccgtgccgta caggcgatcg cgcgtcaggc ccggcacgtc ggcgaccagc
     6001 gcgtcgcggc tcttcgtcag ccggttgaac agggtcgact tgccgacgtt cggccggccg
     6061 acgagaacca gcgtgggctt cacttgtcgg cgacggtgaa ggcgtagagc gcgcctccgc
     6121 gcgtctgagc gaggaagccg ccctcgatgc gcacgaggta cgccgagacc ggcgtgccgt
     6181 cggtctcgac gcgcgcgacg aagccgccgg tgtcgcgcgc gagcaggtgc acgtagccct
     6241 cgacgtcggc gaccgcgacg ctgcgcccga gcggcagggg cgcggtcagc cgccgcagga
     6301 aaagcttgtc ctgcttccac acgctcgtcc cactggagcg gtcgagcgcg tgcaccgatc
     6361 ccttctcgtc gctcacgaag acgaacccgg ggtcgagggc gacgccggcg ctgctcgaga
     6421 gctcgcgcgt ccagaggggg ttggcgctcg cgaggtcgaa gcagccgacc cgtccctggt
     6481 aggcggcggc gcacacgtcg cgctcgccga ccgccggaat cccgaccacg tcgctcacgc
     6541 gctcgagctc ggtcgacccc ttggggaccg ccactgtcgc ttcccagcgc ggcgcgccgt
     6601 tcgcgagcga aagcgccatg agcttcccgc ccgagaaacc cgcatacacg atgcctctgc
     6661 tgacggccat gccctcggtc gcgcgcaccg acagcggcgc ggtcgcccgc tggtagagcc
     6721 accggcgcgt gccgtccttc gcgtcgagag cgaacaggcg gctgtcgccg ctgcgcacga
     6781 gcaccagatc gcccgtgacc accggaggcg acaggacctc gctcgagacg cgggagcgcc
     6841 agcgcgcctt gcccgtcgcg gcgtcgaggg cgaccacctg gccctcctcc gagccgacgg
     6901 cgacgatgac gccgtcggtg ccgacgccgc cgctcagcgc ctcgccggcg tcggcgcgcc
     6961 agcgctcgcg gccgcgcgcc gcgtccaggc tcacgaccgt accgtcgtgc gcggcggcgt
     7021 agacgtaacc cccggccacg acgggcgcga ggacggcttc cttggcgtcg ccgacgcgcg
     7081 cttcccacgc gagccgcacc gaaagcgtcg tcttgatgtc ctgcagctcg gccatcttcg
     7141 ggcccgaaga cccgaagcag ccggcgagcg cgatcgacgc ggcgagcgcc gccccgcggc
     7201 taagagccct caccgagggc atcgagcttg agacgcagcg actcgcgcag cgcgccgtcc
     7261 cggtcgctcg ccttgtcgag agccgagcga taggccgtac gcgcttcggc gggcttcttc
     7321 tggaggacga ggacgtcgcc gcgctcggcc gcgaacagcg cttcgaaggc gggatcgggc
     7381 ttcgcgtcga ggaccttgag cgcctcgtcc ggcgcaccgt cgtcgagcag cacgctggcg
     7441 aggcgcagcc gcgcgatgtc gcgcagctgc gcattgcgcg cgtgatcgac cacccaggcg
     7501 agctgggcgc gcgccgtctt caggtcgccg gcctggaagt gcaccttcgc cgacgtgagc
     7561 gctgcgagcg gcgcgtacgt cgtgcgcggg tagttctcga ggatggcgcc ggccgagtcg
     7621 cgcgtcgcct tcaggtcgtt cgcgcgcgcc gccttctgca gcgtgtcgta aaggccgccg
     7681 gcctgcgccg cctgcgtgcg ctggtaccag gtccacccgt tccatgccgc gaccgcgagc
     7741 agcacgacga tggccgcggt gagcacggcg ttgccgtgcg tcttccacca cgccttcagc
     7801 tcggccagct gctcctgctc ttccagatcg tatgcgctca tttgccgttg ctcttcctcg
     7861 tttccttgtc ctcgccgccg tcgagcagga gcgccgcgac ggcgtcggcg acgccggcca
     7921 gcggcacgcg cgactgctcg cgctcctcgc gcagcgcctt gacgcccgcc gccccggcgc
     7981 gcgcctcgtc gtcgccgacg atgaccgcca cccaggcccc gctcgcgtcc gccttcttca
     8041 tctgcgactt gaagctgccg ccccccgcgt gctgcagcac gccgaacccg gcgtcgcgca
     8101 gcgcctcggc gacgcggaac gcgaagcgcg ccgcgtcttc gccctggttg acgagataga
     8161 cgtcgggcgc ttcgcctttc gcgccctcgc cggccacggc gagcaggcgc tcgatgccca
     8221 tcgcgaatcc gcacgccggc gtcggcttgc cgcccagctg ctcgaacagg ccgtcgtagc
     8281 ggccgcccgc gcacaccgtc ccttgcgcgc ccaaccggtc gctgacccac tcgaacacgg
     8341 tcagattgta atagtcgagg ccgcgcacga ggcgggcatt gatctcgtaa ggctggcccg
     8401 cgtccttgag tatcgcctgg acgccgtcga aatgcttgag cgacgcgtcg cccagcgtgt
     8461 cgatcagccg cggagcctcg gcgatgatcg gctgcatcgc cgggttcttg ctgtcgagga
     8521 cgcgcagcgg attgctgtcc aggcggcgcc gcgagtcggc gtcgagctcg ccttcgcgct
     8581 tcttcaggta ggcgacgagt cccgcgcggt acgccgcccg ctccgcggcc gagccgagcg
     8641 agttcagctc gaggcggacg ccttcgagac cgagatcgtc ccacagcctc cggcacatga
     8701 gcagcagctc ggcgtcgacg tccgggcccg caaagcccag tgcttccgcg ccgacctggt
     8761 ggaactggcg ataacgcccc ttctgcggac gctcgtggcg gtacatgggc ccgaggtaat
     8821 agagccgttg cggcccgttg tagagcaggc tgtgctcgat cgccgcgcgt accgtggacg
     8881 ccgtggcctc gggacgcagg gtgagcgcct cgccgttcag ctcgtcgacg aagctgtaca
     8941 tctccttctc gacgatgtcg gtcgcctcgc cgatcgcccg gcggaagagc tgcgtgctct
     9001 cgagcaatgg ggtgcggatg ttgcgatacc cgtacgatcg cagccaggcg cgcagcagct
     9061 cctcgagcgc ctcccagcgc tcggtctcgt cgggcaggat gtcgttcatc ccgcgcactg
     9121 cctggatcgg ctgcgtcacg gagctcccgt cagaccgcgg tcttgatcgg aatggtcttc
     9181 gccggcggcg cgtcgcggcc cgggctcccg ccgccgtagg tctgctcgac gtagcgctcg
     9241 accagggcct ggaattcctc ggcgatgcgc tcgcccttga gcgtcacggt cttctggccg
     9301 tcgacgtaga ccggagcgac cggccgctcg cccgagccgg gcaggctgat gccgatgttc
     9361 gcctgcttgc tctccccggg gccgttgacg acgcagccca tgaccgcgac gtgcatgtcc
     9421 gcgaccccgg gatagcgagc gcgccactcg ggcatcttct cgcgcaggaa gcgctggatg
     9481 cggtcggcga gctcctggaa ggtcgtgctg gtggtccggc cgcagcccgg acacgcgatc
     9541 accatcggcg cgaacgagcg cagccccatg gtctgcagca gctcctgcgc gaccacgacc
     9601 tcgcgcgtgc ggtcgccgcc gggctcgggc gtgagcgaga tgcggatcgt gtcgccgatg
     9661 ccttcctgca gcagcaccgc gagcgccgcg ctcgacgcga ccacgccctt ggtgcccatc
     9721 ccggcctcgg tgaggccgag gtgcagcggg tagtcacagc gcgaggcgag ctcgcggtag
     9781 accgcgatca ggtcctgcac gccgctcacc ttgcacgaga gcacgatgcg gtcgccgggc
     9841 agcccgagcg cctcggcgcg ggcggcggat tccaacgccg aggcgacgag cgcctcgcgc
     9901 atcacgagat cggcctcgag cggctgcgcg cgcttcgcgt tctcgtccat gaggcgcgcc
     9961 agcagctcct gatcgaggct gccccagttg acgccgatgc gcacgggctt cgcctcgcgg
    10021 cacgcaattt cgatcagggt cgcgaactgc tcatcgcgct tcgagccgcg gccgacgttg
    10081 ccgggattga tgcggtactt gtcgagagcg cgcgcgcaat cgggatgctc ctggaggagc
    10141 ttgtggccgt tgaaatggaa gtcgccgacg agcgggacgt cgcatccggc cgcgtccagc
    10201 cgctcgcgga tgccggcgac cgccgccgcc gcttcggcgg tgttgacggt gacgcgcacc
    10261 agctccgagc cggcgcgcgc gagatcgagc acctgccgga cggtcgcctg cgcgtccgcc
    10321 gtgtccgtgt tggtcatcga ctgcacgacg atcggctcgc cgccgccgat ggcgacgcga
    10381 ccgatgcgca cctggcgcgt ttttcgccgt gcggacccgg gactttgcga catgggcgtt
    10441 acgggagcgt gaagcgtgcg acctcgacct tgacgtaggg caccaggtcg agcggccgat
    10501 cgttgtagac gacgtgcacg gacgcggcgt tgccgatcac caccgagaac ggcggatgcc
    10561 cggatactac gcgccggctg ccggcgtggt tgagctgcgc catgagcagc ttgccgtcac
    10621 cgtcgttgat ctcgacccat gcgtcgtcgt cgaactccat gacgatgcgg ccctgcccgg
    10681 ggatgctctt cacgtccttc gcgaccggcg ccggcgcggc gctccccgcg gtcttcgccg
    10741 gcgtcggggg ggcgttcgcg gtgctcggcg cgggcgctgc cgcgctcgcg atcttcggag
    10801 tcggcgccgc cgatgccgcc gtccccgccg gcctggcggg ctgggcgccg ttctgggcga
    10861 gactggtcgc ggccttcggg atctcgcggg tcggggccgc cgcgggcttc tccgcctgca
    10921 cgcgcgtgac gggagccggc gcagcctccg gggcagccgg gacctgcgcc gcatcggcgt
    10981 ccgcgagctc ggccggggcc gagcgcttgg gcagcggcaa cccgcccgaa tgccattcgt
    11041 acagcacgcc gccgacggcg accagcacga gcgccgacat gaccgcgtac aggcgggtgc
    11101 cgcgccggcg gcgcgtgccg tcggggaaag gaacgcgctt cgcgcgcagg tcgaccgtgg
    11161 ccgcgcccgg gatctgccgg gcctcgagcg acttcagcag cggctcggcg tcgatctcga
    11221 gcagcttcgc gtagccgcgc accatgccgc gcacgaaggt cgttcccggc agccgcgaga
    11281 attcctcggc ctcgagcgct tcgatctggc gcaccgcgta cttgaggcgc tgcgcgacgt
    11341 ccgcgacggt gagcttgcgc tcggcgcgca ggcgcgcgag caggcgcccg gtcgccacgt
    11401 ccgcggtaac cgcgtcgttc attcgtatct ccccttctgc agttcctggt attccgccga
    11461 tccggcgaag cggcgccgaa gctgggtcgc gtacgagcct tcggcgaccc ggtcgccgag
    11521 cttgcgctcg atgcgcaacg cgagccacag cgactcggcg gtcgggtcga ccgccttgtt
    11581 gaagcgctgg accagggcgc gcgcgtcgcg cagctcgccg cggcggtacc tgagctgcgc
    11641 caggccgatc agcgccggcg gatacgtcgg atcgagacgc agcgcgcggt cgtagaagtc
    11701 gagcgcgtcc ttcggattct tcttctgcac gcaccctgcc gcgacggcat acgtcttctg
    11761 cggccgggcg tagagcggat tgcgcaccgc gttcatgaaa tagcgcaccg actcgtcctc
    11821 gcggccggtc ctgcacagga accagccgta gttgtgattg gtgtccgcgt cgttcggatc
    11881 gatgcgcagc gcgcgctcga acgcacgctc ggcctcggaa ttctcgcgca ggtccatctg
    11941 cacgaggccg aggacgttgt acgccggcgc atagccggag tcggcgcgca gcgcgcggcg
    12001 cgattcctcc agcgccacgc cgaggttgcc gcgctggaga taagccgcgg cgagctcggt
    12061 gtgtgcgcgc gcgcgctcgc gcggcggggc cacgtcgccg atgaccggac cggtgtcgtc
    12121 gcccgagccg gagcccgagc ccgcacagcc ggaaagcggc ccggcgacca ggagcgcgag
    12181 cgccagcgac cgcgcgacgc tcacgaggcg agcctcgcca ggcggaccgt cttccgggtg
    12241 cggtcttgga tctgcccggc gagctggccg caggcggcgt cgatctcgtc gccgcgcgtc
    12301 ttgcgcgtcg tcgtcaccac gccggcggcg ttcagcgtgt cctggaagcg ccggatggtc
    12361 tcgcgcggcg agcgctcgaa gccggagtcg ggaaagggat tgaacgggat caggttgatc
    12421 ttgcagggga cccgccgggc gatcccgacc agctcgcgcg cgtgctcgtc accgtcgttc
    12481 accccgcgca gcatgacgta ctcgaaggtg atgaagtcac gcggcgcgcg ctcgagatag
    12541 cggttgcagg cgtcgagcag ctcggccagc gggtacttgc gattgatggg catcagccgg
    12601 tcgcgcagca cgtcgttcgg cgcgtgcagc gacaccgcga gcgccaccgg gcattcgtcg
    12661 cgcagccggt cgatcatcgg cacgatgccc gaggtcgaca ccgtgacccg ccgccgcgac
    12721 agcccgtagg cgtcatcgtc gagcatcagt ccgagcgcgg cgacggtcgg ctcgaagttc
    12781 agcagcggct cgcccatgcc catcatcacg acgttggtga tcctgcgctc gcccgcgacg
    12841 cccgcggcgc cgtccacgct gcggctcgcg agccacagct ggccgacgat ctcaccgacg
    12901 ccgaggttgc ggttgaaccc ctggcggccg gtcgagcaga agccgcacgc gagcgtgcac
    12961 cccgcctgcg tggacacgca cagcgtgccg cgagcggcct cgggaatgaa gacggtttcg
    13021 atcgcgttgt cggcgccgat gtcgagcagc catttgcgcg tgccgtcctc ggccgtggtg
    13081 tcgctcacga tgcgcggcgc gcggaccacc gcctcgcgcg cgagcttctc gcggaaaccc
    13141 ttcgcaaggt cgctcatgcg cgcgaagtcc ggttcgccgg cccggtgcag ccagcgcttg
    13201 agttgccgcg cgcggaacgg tttctcgccc agcccggcga cgaagcgctc gagcgccgcg
    13261 ccgtccagat cgagaagatt gacgctcacg acgtggcgcg ctccggctcg ggcttgcgcg
    13321 cgttggcgac gcggcgcggg aacacctcgc acgcggggaa gaaataggcg atctcgatgc
    13381 gcgcgttctc ggcgctgtcg gacccgtgaa ccgcgttcgc gtcgatgctc tcggcgaagt
    13441 cggcgcgtat ggtgcctttg tcggccttgc gcgggtcggt cgcgcccatc agctcgcggt
    13501 tgcgggcgac cgcgttctcg ccctcgagca cctgcaccat gatcgggccg gaaatcatga
    13561 actgcaccag gtcgcgaaag aagggccgct cccgatggac ggcatagaag ccctcggcct
    13621 cccgctcgga gaggtgcatc atccgggcgg cgacgacccg gagcccggcc ttctcgaaac
    13681 gggagtagat ctgtcctatc acgttcttgg ccacggcgtc gggtttgacg atggacaggg
    13741 ttctttcaat ggccatgcgg ctctccaagt cgatttcctg cgatttctcg cgatgttccc
    13801 gaatttgcta actttatatt ttaacaagct tttgacgccg tggcgccggt tttctgcaaa
    13861 atcgcggcga gccgctgaag cgagccaatg ggatgattca gctatctgtc aggattcgct
    13921 caggattctg aaagcaggcc cgcgtagagt cgatcacgta gtctctttcc tcctctgtgg
    13981 cgtgggggcg aatcgtcccc acgctttttt cttttccaaa tcgtgatcgt atgcaaaccg
    14041 ccgggaagga cgagcccgta ccgcgtgcta tgctgcctcg ggacgcctca ccccggaacg
    14101 gtatgcgagt tctcatcgta gaagacgaca aggcgctggc cgacggcctc acgcgcacgc
    14161 tgaagcaatc gggctatgcg gtcgatcacg ccgcgaccgg cgagctcgcg ctgcgtgcgt
    14221 gcgccgagga gcactacgac ctcatcgtcc tcgacatcag cctgccgggg atcgacggct
    14281 tcgaggtcct gcggcagctg cggcgcaacc accgcgccgg gagcatcctc atcctgaccg
    14341 cgcacgactc cgaaggcgac cgcgtccgcg ggctcgacct cggcgcggac gactacgtga
    14401 ccaagccttt ctcgctgccc gagctggaag cgcgcgtgcg cgcgctcatc cgccgcagcc
    14461 aggccgtgcg cagcccgaag ctgcgcttcg gcggcctgac ggtcgatacc gtcgcgcggc
    14521 gcgcgagcat cggagcggag ccgctcgacc tgacgccgcg cgagtgggcg gtgctcgaat
    14581 acctgctgat gcgcatcggc caggtcgtga gcaaggagca catgctgcag gcgatgtgca
    14641 gctgggacga ctcgctcacg cacaacgccg tcgaggtgta catctcgcgg ctgcgttcca
    14701 agctgcagca cgcgggcatc cgcatccgca ccgtgcgcgg gttcggctat atggtggagg
    14761 acccggatac cgccgaggcc ggcaccccgg tgccggacac cgcggacagg ccgggcatgc
    14821 gctgacagtg ccgcgctccc gcagcctcag gcagcggctg acctattggc tgttcggtcc
    14881 gctgctcctg ctggtcggag ccggaacgtt cgcctcgtac cgcatcgccc tgaacgcggc
    14941 gaacgacgct tacgacagcg cactgctcga tcccgcgctc gcgattgcga gccacgtgcg
    15001 ccgcgccggc ggccgcctgg agctggacct cccgaacgtc gccctcgagg ccctgcgcta
    15061 cgacgcccag gaccgcgtct tcctgcaggt gcgcggcccg ggaggcgagc tgatcgcggg
    15121 cagtccgcag cttcccgctc cgcccgagcg ggtcgggccc gaccgcaaca tcttctacga
    15181 cgcgaagctc gacaccgagc gcgtgcgcgt cgccgcgctc ggtgtgcgcg ccgagaccgg
    15241 catcgtgctc gtgcaagtgg ccgagacttt tgtgaagcgc gacaagctgg tgctggagct
    15301 gcttatcgcg tcgacgctgc cccaggtcat gattgcgatc gccgcggtgg ccctgttctg
    15361 gttcgggatc ggccgcggtc tcaccccgct cgaccgcctg agcgatgaga tcgcggcacg
    15421 ctcgccgcgc gacctgcgcg cggtgcacga ggaaggccag ccgctcgagg tgaagcccct
    15481 cgtcgtcgcg ctgaaccgcc tgctctcgcg gctttccgct gcgatcgagt cgcagcagcg
    15541 cttcatcgcc aacgccgcgc accagctgcg cacgccgctc gcgggtctca agacgcacgc
    15601 cgagctcgcg cgccgtcagc cgtccaccgt cgagctgagg gcgctgctcg acatgatcgc
    15661 gggcgagacc gaacgcacca gccacctgat caaccagctg ctcacgctgg cgcgcgccga
    15721 gccgcaggag gcgcacgccg gcgcgggaca gccgctcgac ctgcacgagc aggtcggccg
    15781 cgacgtgcgc gaatgggtgc agcgcgcggt cgacaagaat atcgacctcg ggttcgagct
    15841 ggaggatgcg tggacgctcg gccagccgct gctggtgcgc gagctggtcg gcaacctcct
    15901 cgacaacgcg cttgcctaca cccagcccgg aggcgccgtg accgtgcgca cccgcgagag
    15961 cggcgccgag gcgaccgtcg aggtcgagga caacgggccc gggatcccgg agagcgagcg
    16021 cgaaaaggtc ttcgagcgct tctaccggat gcccgccacg ggcggcgaag gctgcggcct
    16081 ggggctgtcg atcgtcgcgg agatcgcgga gcgccacggc gctcgggtcg agctcggcgc
    16141 gcccgcgcga ggcaggggca cgctcgtgcg cgtgctgttc ccgcgcctcg cgcgcgaggc
    16201 gctcgccgcg aacgacgccc gcggcggcaa ggcgctcgct agtcgaacgg ctcccagctg
    16261 acgatcacgc cgggctcgcc ggcgtcggcg tggtaggcgc actcgtcgcc cacgcctgga
    16321 accgatacga gatcctcgcc gcagtagagg agcggcagcc gctcgcgccg ccacggcggc
    16381 actctgcgct cctgcagcag gttcttcagc gtgcggcgcg gccgccggca atcgggccgc
    16441 agccgctctc ccccgcgccg ggcccgcacg gtcacggcag cgcttcgaag cttgtcgacg
    16501 ctcagtccct tcccctcctc gggcttgaag cggagcacgc cgccgagcgc gagcagcggc
    16561 agcgcgctct cgccgttcca ggcctcgcac acctcggacg gcgcgcgctc gggctcgaag
    16621 tacacgcgtc cgcggtagcg gcggaagcac catcccgaga cgcgcacgct gacggccgaa
    16681 tcctgccgcg cggcggagag ctgccgcagc agctcgccga gacgcctcgc gccgggcgca
    16741 ccgatcccgt cgcgctcgca gcaatggcgc agcaggttct tggcgcgcgc ctcccccagc
    16801 gtcagcagcg ccaccgcgtc gagcggcatc ccgccgatac gctcgaggtc ctcgcgcgcg
    16861 agcgcgtcgg cgagatcgcc ggcctcgcgc agatggcgcg ccgcgcgcgc gatcgccgcg
    16921 cgcgcggccg ggaactgccg ctccacccgc ggcagcacct cgtgccgcac gaaattgcgg
    16981 cggcgcgcgg tatcgtcatt gctctcgtcc tcgacccacg cgagaccgcg ctcgcgggcg
    17041 cacgcctcga tctcggcgcg cgagacgtcg agcagcgggc gcaggatcct gatggaggat
    17101 ccctcgagca ggcgctcgcc cggcatgccc gccatcccac gcacgccggt gccgcgcgcg
    17161 agctgcagga gcagcgtctc ggcctggtcg tcgcgatgct gcgccagcgc gatcgcatcg
    17221 gcctggacgc gc
//
LOCUS       DATDMW010000183         9104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1425901, whole genome shotgun sequence.
ACCESSION   DATDMW010000183 DATDMW010000000
VERSION     DATDMW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425901"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VI319_08125"
     CDS             complement(<1..299)
                     /locus_tag="VI319_08125"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY6863856.1"
                     /translation="MFDFVAKHKRLLQIILGLMIVPPFAFWGIQWTQREVVGPDTVAK
                     VGGQRITDQEFTEALRQQEDRMRQLLGRNYNPALMESPAMRSELLDGMISQRLLT"
     gene            complement(357..433)
                     /locus_tag="VI319_08130"
     tRNA            complement(357..433)
                     /locus_tag="VI319_08130"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(397..399),aa:Asp,seq:gtc)
     gene            complement(442..517)
                     /locus_tag="VI319_08135"
     tRNA            complement(442..517)
                     /locus_tag="VI319_08135"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
     gene            complement(575..862)
                     /locus_tag="VI319_08140"
     CDS             complement(575..862)
                     /locus_tag="VI319_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY6863857.1"
                     /translation="MRRGCMNKTEIIDHISKSADISKAAAGRALEATVGAIKTAMKRG
                     GMVTLVGFGTFYVGKRAARAGRDPRTGDAIRIKAARVPKFRAGKALKDAVN"
     gene            complement(1190..3514)
                     /gene="lon"
                     /locus_tag="VI319_08145"
                     /pseudo
     CDS             complement(1190..3514)
                     /gene="lon"
                     /locus_tag="VI319_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
     gene            complement(3619..4884)
                     /gene="clpX"
                     /locus_tag="VI319_08150"
     CDS             complement(3619..4884)
                     /gene="clpX"
                     /locus_tag="VI319_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY6863858.1"
                     /translation="MTEKTGGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCND
                     IIREESATDKGGKPAKSDLPTPREICNILDEYVIGQETAKKILSVAVYNHYKRLKHLS
                     KNDEVELAKSNILLVGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGEDVENI
                     IQKLLQNCDYDVDKAKRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTI
                     ASVPPQGGRKHPNQDFVQVDTTNILFICGGAFDGLEKIIRNRSEKSGIGFGADVRSLK
                     NAKDISAVLKTVEPEDLIKYGLIPEFVGRLPVVATLEELDEQALIQILTEPKNALIKQ
                     YQKLFAMEGVDLEVRNQALQAIAKKALARKTGARGLRSILEQVLLDIMFDLPTMENVS
                     KVVVDEGTIEGGGKPLLIYADQPKVAGSA"
     gene            complement(4895..5569)
                     /gene="clpP"
                     /locus_tag="VI319_08155"
     CDS             complement(4895..5569)
                     /gene="clpP"
                     /locus_tag="VI319_08155"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEY6863859.1"
                     /translation="MSGFNWQVTDSSGSSRYEEPQGLGLVPMVIEQSGRGERAYDIYS
                     RLLKERVVFLVGPVNEVTANLIVAQLLFLESENPDKDIFFYINSPGGSVSAGMAIYDT
                     MQFVKPDVSTLCVGQAASMGSLLLAAGAKGKRFALPNSRVMIHQPMGGFQGQASDIEI
                     HAKEILYLRGRLNEILAKHTGQTVERIARDTDRDNFLSAEDSVKYGLVDRVLSSRTEA
                     PATASV"
     gene            complement(5625..6929)
                     /gene="tig"
                     /locus_tag="VI319_08160"
     CDS             complement(5625..6929)
                     /gene="tig"
                     /locus_tag="VI319_08160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY6863860.1"
                     /translation="MQTSIETLSPLERRLNVDVPAEQIDREVEQRLKKLSRTVRLAGF
                     RPGKVPLKIVAQHYGPQVRSEVIGDAVQNAFAEAVRNQNLRVAGYPRIERKEGGDASH
                     VSFSAVFEIYPEVTLGDVAGARIERPALAVGDAEVDKTIEILRKQRSSWTGVERAARS
                     GDRVTVDFTGRIDGAEFAGGSAKDFPVVLGERQMLPEFETALSGVNAGETKTFPVTFP
                     ADYAGREVAGKTAEFEVAVKRVEERKLPAVDTEFAKLLGVADGDVAKMRAEIRANVER
                     EVKKRVENDVKQKVMQALLDTTKIDVPKSLIEMEVQRLVQQTRAELESRGLKSDRLPM
                     GVDAIEQQARRRVTLGLILGELVKAHGLAARPEQVRALVEEHAQSYEQPAEVVKWLYS
                     EPQRLGEFEGLAVESNVVGWVLEHAKVEDKAISFDELMGGAV"
     gene            complement(6951..7035)
                     /locus_tag="VI319_08165"
     tRNA            complement(6951..7035)
                     /locus_tag="VI319_08165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6999..7001),aa:Leu,seq:tag)
     gene            7136..7945
                     /gene="hpnC"
                     /locus_tag="VI319_08170"
     CDS             7136..7945
                     /gene="hpnC"
                     /locus_tag="VI319_08170"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY6863861.1"
                     /translation="MSVGHYENFPVASLLLPRPLRRPVAVIYRFARSADDFADEGDLP
                     AAERLERLEGYRGELRRIAAGRAPGEPLFEELREVIAAHHLPIEPFHDLLDAFAQDVT
                     KKRYASFDEVVDYCRRSADPIGRLMLRLYGQASEKHVLWSDSICSALQLVNFWQDVEL
                     DWAKDRVYLPQDEMARFGVSEGQIAARDAGGSWAALMQFQVERARAMLQRGAPLALAL
                     PGRIGLEIRTVVQGGLRILQKIERVSGDVFRHRPVLRAWDWPLMLSRALLM"
     gene            7958..8791
                     /gene="hpnD"
                     /locus_tag="VI319_08175"
     CDS             7958..8791
                     /gene="hpnD"
                     /locus_tag="VI319_08175"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEY6863862.1"
                     /translation="MTPDEYCQEKAAKSGSSFYYSFLFLPPDRRRAITALYAFCREVD
                     DAVDEPSDAAIARARLAWWRDEVARLFHGTPQHPVSQALAAAVRDFGVTEAGLSEILD
                     GMEMDLAQTRYLDYAALRVYCHRVAGVVGTLAAGIFGASDPRTYEYAERLGLAFQLTN
                     IIRDVGEDARKNRIYLPMDEMQRFGVTAADVLGARETEGFRRLMDFQSARARGCYDEA
                     LALLPAGDRRAQRPGLVMAAIYRALLDEIARDGYRVLRERVALTPIRKLWIAWRTWIT
                     A"
     gene            8788..>9104
                     /locus_tag="VI319_08180"
     CDS             8788..>9104
                     /locus_tag="VI319_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6863863.1"
                     /translation="MTAAARVAVVGGGWAGLAAAAELAARGIPVTVLEAARTLGGRAR
                     RVALEGVELDNGQHVLVGAYRDTLAAMRRVGVDPALALERVPLALEFADGFRMRAPRA
                     PAPL"
BASE COUNT         1302 a         3159 c         2981 g         1662 t
ORIGIN      
        1 tcggtgagca ggcgctgcga gatcatgccg tcgagcagct ccgagcgcat cgcgggggac
       61 tccatgagcg cggggttgta gttgcgcccg aggagctggc gcatccggtc ctcctgctga
      121 cgcagcgcct cggtgaattc ctggtcggtg atccgctgcc cgccgacctt cgccaccgtg
      181 tccgggccga ccacctcgcg ctgcgtccat tgaatgcccc agaacgcgaa cgggggaacg
      241 atcatcagcc ccaggatgat ctgcagcagg cgcttgtgct tggcgacgaa gtcgaacatg
      301 agtacgcggg ccccaaagca atcggggcgc tcgaagcgcc ccggtgatcg tgaatctggc
      361 ggagtggacg ggactcgaac ccgcgacccc cggcgtgaca ggccggtatt ctaaccagct
      421 gaactaccac tcctctcgac ctggtgggtg ctgacgtgat cgaaccgccg acattcgcct
      481 tgtaagggcg acgctctacc agctgagcta agcacccttc accaacagcg cgccaacgag
      541 tgcaccaaca agcgggccca cctcggaagg ccgactagtt tactgcatct ttcaacgcct
      601 tgccagcgcg gaacttgggc acgcgcgcag ccttgatccg gatggcgtcc ccggtgcggg
      661 ggtcgcggcc cgcgcgcgcg gcgcgcttgc cgacatagaa ggtgccgaag cccacgagcg
      721 tcaccatgcc gccgcgcttc atcgcggtct tgatcgcccc gaccgtcgct tcgagcgcgc
      781 gacccgcggc ggccttggag atgtcggcgg acttggagat gtggtcgatg atttcggtct
      841 tgttcatgca tcccctccgg atgtaaggca cgaatcgcga aaaagccgtc cctgcagcgg
      901 cccccgagcg gccgcctggc ttgcaattcc ttccgcttat ttgcttccga acgtgtgacg
      961 gcgcttcgcg gcacgcgagc tgcggcgcta tcacttcgtc aatttatagc gctcgcgaaa
     1021 cggccgtgtc aaggcgcggc atcaggcaag gacgcgactc ggcgccgcgc gcgcggttcg
     1081 tctgctccgc gcgccccggg tgtcgctaat gagtgacaac ggtcgaagag cccttgtcgt
     1141 cggagaccgg cgggaggggc ccgaccttct cgtcggcctt gcgcggctcg ggcttgcgct
     1201 cgagcgcgag ctcgagcacc tggtcgaccc atttcacggg gacgatctcg agcttgttct
     1261 tgatgttgtc cgggatctcg acgaggtcct tcacgttctc ctcggggatc agcaccgtct
     1321 tgatgccgcc gcggtgcgcc gccaggagct tctccttcag gccgccgatc accagcacct
     1381 cgccgcgcag cgtgatctcg ccggtcatgg cgacgtcggc ccgcaccggg atgcccgtca
     1441 gcatcgacac catcgcggtg cagatgccga tgcccgcgct cggcccgtcc ttgggcgtgg
     1501 cgccctcggg caggtggatg tggatgtcgt tcttctggta gaagtcctcg gtcacgccga
     1561 gcttggacga gcggttgcgc accaccgtga gcgcggcctg gatcgactcc tgcatcacct
     1621 cgccgagctt gccggtcgtg atggtcttgc ccttgccggg catggtcacc gcctcgatcg
     1681 tcagcagctc gccgccgacc tcggtccagg cgaggcccgt gacctggccg acctggctct
     1741 gcttctcggc gatgccgtag gtgtagcgcc gcaccccgac gaacttgtcg aggttcttgg
     1801 gcgtgaccag gatcttgtgc ggcttcgacg ccgtgaccag ggtcttcacg accttgcggc
     1861 agatcttgga cacctcgcgc tcgagaccgc gcacgccggc ctcgcgcgtg tagtagcgga
     1921 tcaggtcgcg gatcgccgcg tcggagaccg agatctcgtc cttcttcagg ccgttgttct
     1981 tcagctgctt cggcagcagg tagcgaaccg cgatgttgac tttctcgtcc tcggtgtagc
     2041 ccgagatccg gatgacttcc atccggtcga gcagcggcgg cgggatgttc agcgtattcg
     2101 ccgtcgcgac gaacatgacc tccgagaggt cgtactcgac ctcgacgtag tgatcggtga
     2161 acgtatggtt ctgctcggga tcgagcactt cgagcagcgc cgacgacgga tcgccgcgga
     2221 aatccattcc gagcttgtcc acctcgtcga gcaggaacag cgggttcttc acgccgacct
     2281 tcgacatgtt ctgcaggatc ttgccgggca tcgagccgat gtaggtgcgc cggtgcccgc
     2341 ggatctcggc ttcgtcgcgc acgccgccca gcgacatgcg aacgaacttg cggttcgtcg
     2401 ctttcgcgat cgactgcccg agcgacgtct tgccgacccc gggggcgccc accaggcaca
     2461 ggatcggggc cttcaccttg tcgacgcggc tctgcaccgc gagatactcg acgatgcgct
     2521 ccttcacctt ctcgagcccg tagtgatcgg cgtcgaggat cttctcggcc gtggcgatgt
     2581 cgcgcgagat cttgctgcgc ttcttccacg gcagcccgac gatggtctcg atgtagttgc
     2641 gcaccaccgt cgcctccgcc gacatcggcg acatcaggcg cagcttcttc agctcggcct
     2701 cggccttctt gcgcgcgtcc ttgggcatgc gagccgactt gatgcgcttc tccatctcct
     2761 cgaggtcggc gccgtcctcg ccttcgccga gctccttctg gatcgccttc acctgctcgt
     2821 tgaggtagta ctcgcgctgg ctcttctcca tctggcgctt gacgcgcccg cggatgcgct
     2881 tctcgacctg gaggatgtcg atctcggtct cgagctgcga gagcaggtgc tccagccgct
     2941 gcttgacctc gaacatctcg aggacctgct gcgtctgctc gagcttgagc ggcaggtgcg
     3001 cggcgatcgt gtccgccagg cggcccggct cctcgatgcc cgccagagac gtgaggatct
     3061 cgggcgggat cttcttgttc agcttcacgt actggtcgaa ctgcgcgatc agcgcccggc
     3121 gcatcgcctc gacctcggtg ttgtcgcccg cgtcgggcgg cacggggatc gcgtcggcga
     3181 cgtagtggct gcgcgcgtcg tcgatcgact ggatgcgtgc acgctggccg ccctcgacca
     3241 gcaccttcac cgtgccgtcg ggcagcttca gcatctgcag gatgttcgag acgcagccga
     3301 tcttgtacat gtcctcggcc gccggctcgt ccttggccgc ggacttctgc gcgaccagca
     3361 ggatcgactt gcccgcctcc atcgcgatct ccatcgcctt gatggacttc ggccgcccga
     3421 cgaacagcgg gatcaccatg tgcgggaata ccaccacgtc ccgcaggggc aatagcgggt
     3481 actggttgag ctcaggaagg atttcgttgg ccatgatgct cccgtgcgag tggtacaggc
     3541 actatacaac ttggggcggg cgcccgtgtt tcaatgtgtt tcaggcgccc ccgggacggt
     3601 gcgggcgcgg cgggcaggtc acgcgctgcc ggccaccttg ggttggtcgg cgtagatcag
     3661 cagcggcttg ccgccgccct cgatcgtgcc ttcgtcgacg acgaccttgc tgacgttttc
     3721 catcgtcggc aggtcgaaca tgatgtcgag gagcacctgc tccaggatcg agcgcagtcc
     3781 gcgggcgccg gtcttgcgcg ccagcgcctt cttcgcgatc gcctgcaggg cctggttgcg
     3841 gacctcgagg tcgacgccct ccatcgcgaa cagcttctgg tactgcttga tgagggcgtt
     3901 cttcggctcg gtgaggatct ggatcagcgc ctgctcgtcg agctcctcga gcgtcgccac
     3961 gaccggcagc cgcccgacga actcggggat gaggccgtac ttgatcaggt cctcgggctc
     4021 cacggtcttc agcacagcgc tgatgtcctt cgcgttcttc aggctgcgca cgtcggcgcc
     4081 gaagccgatg ccgctcttct cggagcggtt gcggatgatc ttctcgagac cgtcgaacgc
     4141 cccgccgcag atgaacagga tgttcgtggt gtcgacctgg acgaagtcct ggttcgggtg
     4201 cttgcgcccg ccctgcggcg gcaccgaggc gatcgtgccc tcgatcagct tgagcagcgc
     4261 ctgctgcacg ccctcgcccg agacgtcgcg ggtgatcgac gggttgtccg acttgcgcga
     4321 gatcttgtcg atctcgtcga tatagacgat gccgcgcttc gccttgtcga cgtcgtagtc
     4381 gcagttctgc agcagcttct ggatgatgtt ctcgacgtcc tcgccgacgt agcccgcttc
     4441 cgtgagcgtg gtcgcgtcgg cgatcacgaa cggcacgttc agcaggcgcg ccagcgtctg
     4501 cgcgagcagc gtcttgcccg agcccgtggg gccgacgagc aggatgttcg acttcgcgag
     4561 ctcgacctcg tcgttcttcg acaggtgctt gagccgcttg taatggttgt agacggcgac
     4621 cgacaggatc ttcttcgccg tctcctgccc gatcacgtac tcgtcgagga tgttgcagat
     4681 ctcgcgcggc gtcggcaggt ccgacttcgc gggcttgccg cccttgtcgg tcgcgctctc
     4741 ctcgcggatg atgtcgttgc agagctcgat gcactcgtcg cagatgaaca ccgagggacc
     4801 ggcgatcagc ttccgcacct cgtgctggct cttgccgcag aaggagcagt agagaagctt
     4861 ctcgccgccg gtcttttcgg tcattccggt tccctcacac gctcgccgtc gccggcgcct
     4921 cggtgcgcga gctcagcacg cggtcgacca ggccgtactt caccgaatcc tccgccgaga
     4981 ggaaattgtc gcggtcggtg tcgcgcgcga tgcgctcgac ggtctggccc gtgtgcttgg
     5041 ccaggatctc gttcaaccgc ccgcgcaggt acaggatctc cttggcgtga atctcgatgt
     5101 cggaggcctg gccctggaag ccgcccatcg gctggtggat catcacgcgc gagttgggca
     5161 gcgcgaagcg cttgcccttg gcgcccgcgg cgagcagcag cgatcccatg ctcgccgcct
     5221 ggccgacgca cagcgtcgac acgtccggct tcacgaactg catggtgtcg tagatggcca
     5281 tgccggccga taccgagccg cccggcgagt tgatgtagaa gaagatgtcc ttgtccgggt
     5341 tctcggactc gaggaacagc aactgcgcga cgatcaggtt cgcggtgacc tcgttgaccg
     5401 ggccgaccag gaaaaccacc cgttccttca gcaagcggga gtagatgtcg taggcgcgct
     5461 cgccgcggcc gctctgctcg atgaccatcg gcaccaagcc gaggccctgc ggctcctcgt
     5521 aacgggagga gccagaagaa tcagtgactt gccagttgaa tccgctcatg cgttcgtctc
     5581 cgagccaacg aaagctatcc taccactatg gcgccggccc ggcgtcaaac cgcacccccc
     5641 atcaactcgt cgaaactgat cgccttgtcc tccactttcg cgtgctcgag cacccagccg
     5701 accacgttgg actccaccgc aaggccctcg aactcgccca gccgctgcgg ctcggaatag
     5761 agccatttta ccacctcggc cggctgctca tagctctgcg cgtgctcctc gaccagcgcg
     5821 cgcacctgct cggggcgggc ggcgaggccg tgcgccttca ccagctcgcc gaggatcagc
     5881 ccgagcgtga cgcgccggcg cgcctgctgc tcgatcgcgt cgacgcccat cggcagacgg
     5941 tccgatttca ggccgcggct ctcgagctcg gcgcgcgtct gctgaaccag gcgctgcacc
     6001 tccatctcga tcagcgactt gggcacgtcg atcttcgtcg tgtcgagcag cgcctgcatc
     6061 accttctgct tgacgtcgtt ctcgacgcgc ttcttcacct cgcgctcgac gttcgcccgg
     6121 atctccgcgc gcatcttcgc gacgtcgccg tcggcgacgc ccagcagctt cgcgaactcc
     6181 gtatccaccg ccgggagctt gcgctcctcg acgcgcttca ccgcgacctc gaactcggcg
     6241 gtcttccccg cgacctcgcg gcccgcgtaa tccgcgggga acgtcaccgg gaacgtcttg
     6301 gtctctcccg cgttcacgcc cgagagcgcg gtctcgaact cgggcagcat ctggcgctcg
     6361 cccagcacga ccgggaagtc cttggccgag ccgccggcga actcggcgcc gtcgatgcgg
     6421 cccgtgaagt cgaccgtgac gcggtcgccg ctgcgcgcgg cgcgctcgac gcccgtccag
     6481 ctcgagcgct gcttgcgcag gatctcgatc gtcttgtcga cctcggcgtc gcccaccgcg
     6541 agcgcgggcc gctcgatgcg ggcgcccgcg acgtcgccca gcgtcacctc ggggtagatc
     6601 tcgaacacgg cgctgaagga gacgtgcgac gcgtcgccgc cctccttgcg ctcgatccgc
     6661 gggtagccgg cgacgcgcag gttctggttg cgcaccgcct cggcgaacgc gttctgcacc
     6721 gcgtcgccga tcacttccga gcgcacctgc ggcccgtaat gctgcgcgac gatcttgagc
     6781 ggcaccttgc ccggccggaa gccggcgagc cgcaccgtgc gcgacagttt cttcaggcgc
     6841 tgctcgacct cgcggtcgat ctgctctgcg gggacgtcga cgttcaggcg ccgctcgaga
     6901 gggctcaggg tttcgatgct ggtctgcatg ccgcgttcat ccggtcgtcg tggtgcgaag
     6961 gaagggactc gaacccccat gccttgcggc gctggaacct aaatccagtg cgtctaccaa
     7021 ttccgccacc ttcgcatcgg cgtgcggtcg ctcgcggccc gggagggctg tgattctacc
     7081 gtaaggcccc tatactgtca gcctgaagcg gggaaaaccc tgcaaaaaca tggaaatgtc
     7141 cgtcgggcat tacgaaaact tcccggtcgc ttcgctgctg ctgccgaggc ctttgcgccg
     7201 gccggtcgcg gtcatctacc gcttcgcgcg cagcgccgac gacttcgccg acgagggcga
     7261 tctcccggcc gcggagcggc tcgagcgcct cgagggctat cggggggagc tgcggcgcat
     7321 cgccgcggga cgcgcgccgg gcgagccgct gttcgaggag ctccgcgaag tcatcgccgc
     7381 gcatcatctc cccatcgagc cgttccatga cctgctcgac gccttcgcgc aggacgtgac
     7441 gaagaagcgc tatgcgagct tcgacgaggt cgtcgactac tgtcgccgct ccgccgatcc
     7501 gatcggccgg ctgatgctgc gcctctacgg gcaagcgagc gagaagcacg tgctgtggtc
     7561 ggactcgatc tgctcggcgc tgcagctcgt caacttctgg caggacgtcg aactcgactg
     7621 ggcgaaggac cgcgtctatc tgccgcagga cgagatggcg cgcttcggcg tgagcgaggg
     7681 ccagatcgcc gcacgcgacg cgggcggcag ttgggctgcg ctcatgcagt tccaggtcga
     7741 gcgcgcccgt gccatgctcc agcgcggcgc gccgctcgcg ctcgcgctcc cgggaaggat
     7801 cggcctcgag atccgcacgg tggtccaggg gggcctgcgc atcctgcaga agatcgagcg
     7861 cgtgagcggc gacgtgttcc ggcatcggcc cgtgctgcgc gcctgggact ggccgctgat
     7921 gctctcgcgc gcgcttttga tgtaaacgct cgatgcgatg acccccgacg agtactgcca
     7981 ggagaaggcg gcgaagagcg gatcgagctt ctactactcg ttcctgttcc tgccgccgga
     8041 tcggcgccgc gccatcaccg ccctctacgc gttctgccgc gaggtcgacg acgcggtcga
     8101 cgagccgagc gacgccgcga tcgcgcgcgc gcgcctcgcg tggtggcgcg acgaggtcgc
     8161 gcggctcttc cacggcacgc cgcagcaccc ggtgtcgcag gccctcgcgg ccgcggtgcg
     8221 cgacttcggc gtcaccgagg ccggcctgtc cgagatcctg gacggcatgg agatggatct
     8281 cgcgcagacg cgctacctcg actacgccgc gctgcgcgtc tattgccacc gcgtcgccgg
     8341 cgtcgtcggc acgctggccg ccggcatctt cggcgcgagc gacccgcgga cctacgaata
     8401 cgccgagcgc ctcgggctcg cgttccagct cacgaacatc atccgcgacg tcggcgagga
     8461 cgcgcgcaag aaccgcatct acctgccgat ggacgagatg cagcgcttcg gcgtcaccgc
     8521 ggcggacgtc ctgggcgcgc gcgagaccga gggcttccgg cgtctcatgg acttccagtc
     8581 ggcgcgcgcg cgcggctgct acgacgaggc gctggcgctg ctccccgcgg gcgaccggcg
     8641 cgcgcagcgc ccgggcctgg tgatggccgc gatctaccgc gcgctgctcg acgagatcgc
     8701 gcgcgacggc taccgcgtcc tgcgcgagcg cgtggcgctc acgccgatcc gcaagctctg
     8761 gatcgcctgg cgcacctgga tcacggcgtg acggccgccg ccagggtggc cgtggtcggc
     8821 ggcggctggg ccggcctcgc cgccgccgcc gagctcgcgg cgcgcggcat cccggtcacg
     8881 gtgctcgagg cggcgcgcac gctcggcggg cgcgcgcgcc gcgtcgcgct cgagggcgtc
     8941 gagctcgaca acggtcagca cgtcctggtc ggcgcctacc gcgacacgct cgccgcgatg
     9001 cgccgggtgg gagtggaccc ggcgcttgcg ctcgagcgcg tgccgctcgc gctcgagttc
     9061 gccgacgggt tccggatgcg ggcgccgcgc gccccggctc cgct
//
LOCUS       DATDMW010000184         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_678047, whole genome shotgun sequence.
ACCESSION   DATDMW010000184 DATDMW010000000
VERSION     DATDMW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678047"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..461
                     /locus_tag="VI319_08185"
     CDS             207..461
                     /locus_tag="VI319_08185"
                     /inference="COORDINATES: protein motif:HMM:NF025653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAN domain-containing protein"
                     /protein_id="HEY6863864.1"
                     /translation="MELAKTKAMNRRGSDYRSFEARGAAQCAATCAGERQCRAWTWTR
                     PAAPEAQGRCWLKDDAPAPAKSACCISGVKATRPDVARRR"
     gene            472..3474
                     /locus_tag="VI319_08190"
     CDS             472..3474
                     /locus_tag="VI319_08190"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6863865.1"
                     /translation="MSTTPTGDGGASAQSAEQALRALERAYVERSYGYRDDADIIALA
                     VEFARAGGPLADDPDLMALACLDARFPEERHRALGALHAALEARAPGWALTTHGEAIA
                     LDLLDGEARRYLQPGNSVGRLVGLVNALIEDFGARPWLGPFAPDGAETRLDLLDELAE
                     DTEWLRQACAGYLAWREHPSRSDQPGQPRARPRQTDADRELELAYEEFETARRAHGAG
                     EPGAETHFRSALAAFERRLPDSRWLATARNELGVVLKEKGRPRDAAALFRMAHEYFEA
                     RHPEDEPDVAVTLHNLGAALSQLGDAKAAGYLERALQLQLTHGRTRPIDIANNCQTLG
                     ENQHARGLLREARSSFEQGLAILDRMPERPLKAIGDACAHLCEVTQELGDLAAAESYA
                     RRLVAARTEALGPFHRDAADSLNALATVLHSQGRLREAEETLRLALGRFEEGPGRYDA
                     YAINTRINLAQVLHWRGDADSADRTVREALEASEHAEGRDSARTARALSVLGEILNDR
                     GLHGDAVPVLTRARDIYVRERGPDHVDTAYCMNRLGLAFRGCGALAEAVNMHEHAEAV
                     LARRRGPDHVDTLTARSNRAVALEMQGRAQDAEAVWADVVARSARIAGAQALKGEAAY
                     RMAESLRARGELAAARGRYLEALEAFEGRDDTANPGIICEALARLHASAGEFDRAEHY
                     LGRVPPIYARVYGQLHEETIRVGSEFVEVLARRDVPAAARIASDRLAAVRKHREAPAD
                     MLARLSLQAGRLLRRAGDARQARSLLQEAHLSILRSGALRPDAETAIDVAFELAGAHA
                     EVKDGASRVGTLRRALGRCDELPDADEGSVAALCLALALALVDGGDREQAHVAAVRAW
                     SYFRSARGDPDAAASAFTAYWQTAPERDVRPAAWCAHLEAGGASATWRLLLEFSTEDG
                     RELDCILGALSEHIPAEVERVGSRLTLSSPPGAPLDAQTDLVYALAAYPRLSWARWTI
                     WCDAGTGEAMRAQGTDAADFLAIVRAPR"
BASE COUNT          501 a         1277 c         1288 g          459 t
ORIGIN      
        1 gcaccgcgtc tccgtgaagt tcggggtgtg gcgctgtcac cggtcggcaa acctgcttgc
       61 gggcgacgga gagtccgatt cgaacgtgtc ggcgtacggg ctggacatct acatctacgg
      121 gccggtcggc gtcgatccct acaattgagg catcggctgc agaggcagca tcgccttcac
      181 accaggctgg tgtcgacatc gaccacgtgg aacttgcgaa gacgaaggcg atgaaccgcc
      241 gcggctcgga ttaccgcagc ttcgaggcgc gcggtgccgc gcagtgcgcg gcgacgtgcg
      301 cgggcgagcg acagtgccgc gcgtggacgt ggacccgacc ggccgcgccg gaggcgcagg
      361 ggcgctgctg gctcaaggac gatgcgcccg cgcccgcgaa gagcgcgtgc tgcatctccg
      421 gagtgaaagc gacgaggccg gacgtcgcca ggcgtcgcta gcccgcaagt catgtccacg
      481 accccgaccg gagacggcgg cgcatcggcg caatccgccg agcaggcgct gcgcgcactc
      541 gagcgcgcct acgtcgagcg cagctacggc tatcgcgacg acgccgacat catcgcgctc
      601 gcggtcgagt tcgcccgcgc cggcgggccg ctcgccgacg acccggacct catggctctc
      661 gcctgcctcg atgcgcgctt ccccgaggag cgccaccgcg cgctcggggc gctccacgcg
      721 gcgctcgagg cccgcgcccc gggatgggcg ctcaccacgc acggcgaagc gatcgcgctc
      781 gacctcctcg acggcgaagc gcgccggtac ctgcagcccg gcaattccgt gggacggctg
      841 gtcgggctcg tcaatgcgct catcgaggat ttcggcgcgc ggccatggct cgggccgttc
      901 gcacccgacg gcgcggaaac gcggctcgat ctcctcgacg agctcgccga ggacaccgag
      961 tggctgcgcc aggcctgcgc ggggtatctc gcgtggcgcg agcatccgtc gcggtccgat
     1021 cagcctgggc agccgcgcgc ccgcccaagg cagacagacg cggaccgcga gctcgagctc
     1081 gcctacgagg aattcgagac ggcccgccgg gcgcacggcg ccggcgagcc cggcgccgag
     1141 acgcatttcc gctcggcgct cgccgcgttc gagcgccggc tgcccgattc gcggtggctc
     1201 gcgacggcgc gcaacgagct cggggtcgtg ctgaaggaga aggggcgccc gcgcgacgcc
     1261 gccgcgctgt tccgcatggc gcacgagtat ttcgaggccc gtcaccctga agacgagccc
     1321 gacgtggccg tcacgctgca caacctcggc gcggcgctga gccagctggg cgatgccaag
     1381 gccgcaggat acctggagcg ggcgctgcag ctgcagctga cgcacggccg caccaggccg
     1441 atcgacatcg cgaacaactg tcagacgctg ggcgaaaacc agcacgctcg cggcctcctg
     1501 cgggaggcac ggagcagctt cgagcagggc ctcgcgatcc tggaccggat gcccgagcgc
     1561 ccgctcaagg ccatcggcga cgcgtgcgcc cacctgtgcg aggtgacgca ggagctcggc
     1621 gacctggccg ccgccgaatc ctacgcgcgc cgcctggtcg cggcgcgcac cgaggcgctc
     1681 ggcccgttcc accgcgatgc cgcggattcg ctgaacgcgc tcgcgaccgt gctgcattcg
     1741 cagggccggc tgcgcgaggc cgaggagacg ctgcgcctcg cgctcggccg cttcgaggaa
     1801 ggcccgggtc ggtacgatgc ctacgccatc aacacgcgca tcaacctcgc gcaagtcctg
     1861 cactggcgcg gcgacgccga cagcgccgac cgcaccgtgc gcgaagcgct ggaggcgagc
     1921 gagcacgccg agggcagaga cagcgcacgc acggcgaggg ccctttccgt gctcggcgag
     1981 atcctcaacg accgcggcct gcacggcgac gccgtcccgg tcctcacgcg cgcgcgcgac
     2041 atctacgtcc gcgagcgcgg gcccgatcac gtcgacacgg cgtactgcat gaaccggctg
     2101 gggctcgcct tccggggctg cggagcgctg gccgaggcgg tgaacatgca cgagcacgcc
     2161 gaggccgtcc tcgcgcgccg ccgtggcccg gatcacgtcg acacgctcac ggcgcgcagc
     2221 aatcgggccg tggcgctcga gatgcaggga cgggcgcagg acgcagaggc cgtgtgggcc
     2281 gacgtggtgg cgcgctcggc gcgcatcgcc ggggcgcaag cgctgaaggg cgaggccgcg
     2341 tatcggatgg ccgagtcgct gcgggcgcgt ggcgagctcg ccgcggcccg cggacgctat
     2401 ctcgaggcgc tggaggcgtt cgagggccgg gacgacaccg ccaatccggg cattatctgc
     2461 gaggcgctcg cccggctgca cgcgagcgcc ggcgagttcg atcgcgccga gcactatctc
     2521 ggccgggtgc ccccgatcta cgcccgcgtc tacggccagc tgcacgagga gacgatccgc
     2581 gtcggcagcg agttcgtgga ggtccttgcg aggcgggacg tgcccgccgc ggcgcgcatc
     2641 gcatccgatc ggctcgccgc ggtccgcaag caccgcgaag cgccggccga catgcttgcc
     2701 cggctttcgc tccaggcggg acgtctgctg cggcgcgccg gcgacgcgcg ccaggcgcgc
     2761 agcctgctgc aggaggccca tctatccatc ctgcgctcgg gcgcgctccg cccggatgcc
     2821 gagaccgcga tcgacgtcgc gttcgagctc gccggcgctc acgccgaggt gaaagacggc
     2881 gcctcccggg tcggcaccct gcggcgcgcg ctcggccgct gcgacgagct cccggacgcc
     2941 gacgaagggt ccgtcgccgc gctgtgcctc gccctcgcgc ttgcgctcgt cgacggcggc
     3001 gatcgcgaac aggctcacgt cgcggcagtg cgcgcctggt cgtatttccg cagcgctcgc
     3061 ggcgatccgg acgccgcggc gtcggcgttc acggcatact ggcagaccgc cccggagcgt
     3121 gacgttcggc ccgcagcgtg gtgcgctcac ctggaggcgg gcggagcgag cgcgacatgg
     3181 cggctgctgc tcgagttctc cacggaagac gggcgcgagc tcgattgcat cctcggcgcg
     3241 ctcagcgaac acatcccggc cgaggtcgaa cgcgtcggat cccggcttac gctgtcgtcg
     3301 ccgccgggcg cgccgctcga tgcgcagacc gatctggtgt acgcgctcgc cgcgtatccg
     3361 cggctttcgt gggcgcgctg gacgatctgg tgcgacgccg gcacgggcga ggcgatgcgt
     3421 gcccaaggca ccgacgccgc cgacttcctg gcgatagtac gcgcgccgcg ctgaggtagg
     3481 actgcaggtt ctgcgtcgtt tcgagcttct tgcgctcggt gatgt
//
LOCUS       DATDMW010000185         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1426220, whole genome shotgun sequence.
ACCESSION   DATDMW010000185 DATDMW010000000
VERSION     DATDMW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426220"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..86)
                     /locus_tag="VI319_08195"
     CDS             complement(<1..86)
                     /locus_tag="VI319_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY6863866.1"
                     /translation="MSFRIGQGFDSHALAAGRRLVIGGVEIP"
     gene            complement(83..775)
                     /gene="ispD"
                     /locus_tag="VI319_08200"
     CDS             complement(83..775)
                     /gene="ispD"
                     /locus_tag="VI319_08200"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY6863867.1"
                     /translation="MAARTFGLIPAAGSGSRLGSAAPKQYLSLAGRPMLHHAIAGLLA
                     DKRVEMVFVVLAPDDAAFRTLDWSAFGERVAPLWCGGASRRDTVLNGLIAAASAVEPN
                     DWMLVHDAARPCLGKVELARLIDSASRDEVGGILAVPVADTLKRADAEKRIAATEPRD
                     GLWQAQTPQMFRHGLLLRALRGAPLVTDEAAAVEGLGYRPLLVEGSARNVKVTFPSDL
                     ALAERFLEENLR"
     gene            861..>2019
                     /locus_tag="VI319_08205"
     CDS             861..>2019
                     /locus_tag="VI319_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY6863868.1"
                     /translation="MTDIATASLSGHRPAVAPKLAGSADALALARLAAASRPLVVFTA
                     DAGEAQRLRDEIPWFAPELQVALLPDWETLPYDSFSPHHDLVSERLATLYRITRGEFD
                     VAIVPATTALYRMGPPAYLAAYSFFVKKGDRLAVQPLRSQLVTAGYTHVTQVVAPGEY
                     CVRGGLIDLFPMGSALPFRIDLADDEIESIRTFDPDTQRTVYQVPEIRLLPAREFPMD
                     DAGRTRFRAHFRETFEGDPSKSSIYKDVSNGIAPAGIEYFLPLFFDQSATLFDYLPEG
                     TRLWLPPDLHEAFTRFGRDTQSRYQVLKGDRTRPVLPPSDLFLSAENFFAEAKRFARA
                     ETPDVRAEPLPDLAVERRAADPLHRLKDFLAGFSGRTLVLAESPGRRETITQ"
BASE COUNT          271 a          742 c          701 g          305 t
ORIGIN      
        1 tgcgggatct cgacgccgcc gatgacgagc ctgcgccccg cggcgagcgc gtggctgtcg
       61 aagccctggc cgatgcggaa gctcatcgga ggttctcctc gagaaagcgc tccgcgagcg
      121 cgagatcgga gggaaaggtc accttgacgt tgcgcgcgct gccttcgacg agcagcggcc
      181 ggtacccgag gccctcgacc gccgccgcct cgtcggtgac gagcggcgcg ccgcgcagcg
      241 cgcgcagcag cagcccgtgc cggaacatct gcggcgtctg cgcctgccac aggccgtcgc
      301 gcggctcggt cgcggcgatg cgcttctccg cgtcggcgcg cttgagcgtg tcggcgaccg
      361 gcacggcgag gatgccgccg acctcgtcgc gcgacgcgct atcgatcagg cgcgcgagct
      421 cgaccttgcc gaggcacggg cgcgccgcgt cgtgcaccag catccagtcg ttcggctcga
      481 ccgccgacgc cgcggcgatg aggccgttca gcacggtgtc gcggcgcgag gcgccgccgc
      541 accacagcgg cgcgacgcgc tcgccgaagg cgctccagtc gagcgtgcgg aacgccgcgt
      601 cgtcgggcgc gagcaccacg aacaccatct cgacgcgctt gtcggcgagc aggccggcga
      661 tcgcgtggtg gagcatgggc cggccggcga gggagaggta ctgcttgggg gcggcggatc
      721 cgagccggga acccgagccg gccgcgggga tcaatccgaa ggttcgtgcc gccacggttt
      781 ataattatat ccgtgtcccg cgtcggggct cgccgcgcga aatgcgcggc gaggtcttac
      841 gcgggttttg ccgtttctgc atgaccgaca tcgccaccgc atctttgtcc gggcaccgcc
      901 ccgcggtcgc tccgaagctc gccggctccg ccgacgcgct ggcgctcgcg cggctggcgg
      961 cggcttcgcg tccgctggtc gtgttcaccg ccgacgccgg cgaagcccag cgcctgcgcg
     1021 acgagatccc ctggttcgcc cccgagctgc aggtggcgct gctccccgac tgggagacgc
     1081 tgccctacga cagcttctcg ccgcaccacg acctcgtgtc ggagcgcctc gcgacgctgt
     1141 accgcatcac gcgcggcgag ttcgacgtcg cgatcgtccc cgccacgacc gcgctctacc
     1201 gcatggggcc gccggcgtac ctcgccgcgt acagcttctt cgtgaagaag ggcgaccggc
     1261 tcgcggtgca gccgctgcgc tcgcagctcg tgaccgcggg ctacacgcac gtgacgcagg
     1321 tggtcgcgcc cggcgagtac tgcgtgcgcg gcggcctgat cgacctcttc ccgatgggct
     1381 cggcgctgcc cttccgcatc gatctcgccg acgacgagat cgagtcgatc cgcaccttcg
     1441 acccggacac gcagcgcacg gtctaccagg tgcccgagat ccgcctcctg cccgcgcgcg
     1501 agttcccgat ggacgacgcc gggcgcaccc gatttcgcgc gcattttcgt gaaacgttcg
     1561 agggcgatcc gtcgaagtcg tcgatctaca aggacgtgtc gaacggcatc gcgccggcgg
     1621 ggatcgagta tttcctgccg ctgttcttcg accagagcgc gacgctgttc gactacctgc
     1681 ccgagggcac gaggctgtgg ctgccgcccg atctgcacga ggcgttcacg cgcttcgggc
     1741 gcgacacgca gtcgcgctac caggtgctca aaggcgaccg cacgcggccg gtgctgccgc
     1801 cgtccgatct gttcctctcc gccgagaatt tcttcgccga ggcgaagcgc tttgcccgcg
     1861 ccgagacgcc cgacgtgcgc gccgagccgc tgcccgatct cgcggtcgag cgccgcgcgg
     1921 ccgatccgct gcacaggctc aaggacttcc tcgcggggtt ttccggccgc acgctcgtgc
     1981 tcgccgagtc gcccgggcgg cgcgagacga tcacgcagt
//
LOCUS       DATDMW010000186         3762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1801089, whole genome shotgun sequence.
ACCESSION   DATDMW010000186 DATDMW010000000
VERSION     DATDMW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801089"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VI319_08210"
     CDS             <1..450
                     /locus_tag="VI319_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6863869.1"
                     /translation="IEDDGFVLWEPAAIILYLARRNAARLWPSTVHGEGRMLQWAFFT
                     ATQIEPPMNTLHEHRVRLPERERNAAIADNCQRRLLEKLGVLEVQLAATPYFGGVRWD
                     LSDFIVASALYAIYEMKLDLVQVPKVDRWLTRSVQRPAAREAIMLRA"
     gene            complement(447..632)
                     /locus_tag="VI319_08215"
     CDS             complement(447..632)
                     /locus_tag="VI319_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863870.1"
                     /translation="MRNRIATLTRWLDRHCPECETEQAHVNDGTREQMYWNYGYLAAL
                     RDVLRKLGGKPGKPGVT"
     gene            complement(770..1921)
                     /locus_tag="VI319_08220"
     CDS             complement(770..1921)
                     /locus_tag="VI319_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6863871.1"
                     /translation="MRIAVVGAGPAGLLFALLARRRFPACSVDVVEQNPVGATYGFGV
                     VFSAGALAFLERDAPDVLAALSAAMETWPVQRIVHRGERVDIDGNGFAAIARLRLLEI
                     LGRLCADAGVAVRWDTTLASLDELGAADLVVGADGFNSLVRRSLADRFGTEVRQLTNR
                     FVWYGTAQPFDCLTLTFRASADGAFVAHHYRYAPGMSTFIVECGDATWRRAGLAAMSD
                     AQSRAYCERVFADDLGGRPLVSNKSVWRQFPLLSARRWWTESGVGHAVLIGDALRTVH
                     FSIGSGTRLAFEDAIALDRAFGEAGLDVPAALAAFERERRPVVEKMLAAASASSHWYE
                     GLAGKMSLDPLSLAYDYMTRSGRMTGARLRETAPKFMKKFDDAQRGWSS"
     gene            complement(1929..2525)
                     /locus_tag="VI319_08225"
     CDS             complement(1929..2525)
                     /locus_tag="VI319_08225"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY6863872.1"
                     /translation="MSRENADAPERAPTVILFAETRGFTRMSEMLDPAVALARVSEFF
                     GLVSAAVEQHEGVVLHLLNDTVMATFTGADDAPHAVDAARRIQSEFALLAERWQSEFG
                     LKAAVAMGLHSGDAVLGYTDGALADRLLVLGDCVSVAERLLHRARSGEFVFSEGVMEV
                     LRASGYDLEAAPLPPLEMARREPIHLFGVLLDTRLDFT"
     gene            2669..3643
                     /locus_tag="VI319_08230"
     CDS             2669..3643
                     /locus_tag="VI319_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6863873.1"
                     /translation="MNAALLRLARACIVALAALPAAPAGADDYPKGPINLVIPLAPGD
                     ATDTSGRGIAEELARELKVPVVPVNRPGGGGSVGTESVVKAKPDGHTITLTNNAAVIY
                     RSILDPKNVTYDPLRDLTPLGLAMRSPTVIAVRGDAPYKTLAELVAYSKQHPGAVRIG
                     TAGSGSVGEFCVRTITSLTGADLTLVPFTGASPGVTALLGGHVEGIALALGTMTGHLR
                     SGAFRGMVISSKYPDFPGIPTMAELGYKEPLFDVWVAFFAPAGVPPEVTSVLVPALER
                     AIKSPPIAAKLKPLGMILDYVPPDALVEQIRVEHRRVEAIASKAGLVK"
BASE COUNT          580 a         1362 c         1288 g          532 t
ORIGIN      
        1 atcgaggacg acggcttcgt gctgtgggaa ccggcggcga tcatcctgta cctcgcccgc
       61 aggaatgcgg cgcgcctgtg gccgagcacc gtccacggcg aaggccggat gctgcaatgg
      121 gcgttcttca ccgccaccca gatcgagccg ccgatgaaca cgctgcacga gcatcgcgtg
      181 cgcctgcccg agcgtgagcg caacgccgcg atcgccgaca actgccagcg gcggctgctc
      241 gaaaagctcg gcgtcctgga ggtccagctc gcggcgacgc cgtactttgg aggcgtccgc
      301 tgggacctgt ccgacttcat cgtcgcgagc gcgctctacg cgatctacga gatgaagctc
      361 gacctcgtcc aggtcccgaa ggtggatcga tggctcacgc gcagcgtaca gcggcctgcg
      421 gcgcgcgaag cgatcatgct cagggcttaa gttactcccg gtttgccggg cttgccgccc
      481 agcttgcgca gcacgtcgcg cagcgcggcc aggtagccgt aattccagta catctgctcg
      541 cgtgtgccgt cgttgacgtg cgcctgctcg gtctcgcatt cggggcagtg ccggtccagc
      601 caacgcgtca gtgtggcgat tcggttacgc aagtcttgta agacttttgt agaaagtgcc
      661 acggtcgtcg ctccgtattt tcttcttcaa aggagcttcg accacggcgc gacgcgcggt
      721 tccatccgcc ggttactccg tttgggagcg tcgtccggtc gcgacttcgt cacgaagacc
      781 agccccgctg cgcatcgtcg aactttttca tgaacttggg cgcggtttcg cgcagtctcg
      841 ccccggtcat gcgcccgctt cgggtcatgt agtcgtaggc gagcgacagc ggatcgagcg
      901 acattttccc cgccagtccc tcgtaccaat gcgagctcgc actggcggcc gcgagcatct
      961 tctcgacaac ggggcggcgc tcgcgctcga acgccgcgag cgccgccggc acgtccagcc
     1021 ccgcttcgcc gaaggcgcgg tcgagcgcga tcgcgtcctc gaatgccagg cgcgtacccg
     1081 agccgatgga gaaatgcacc gtgcgcagcg cgtctccgat caggacggcg tgtcccaccc
     1141 ccgactccgt ccaccagcgc cgggcggaca ggagcggaaa ctgccgccat acggatttat
     1201 tggacacgag cggacgcccg ccgagatcgt cggcgaatac gcgctcgcag tacgcgcggc
     1261 tctgcgcgtc ggacatcgcc gcgagccccg cgcgcctcca ggtcgcgtcg ccgcactcga
     1321 cgatgaaggt gctcatgccc ggcgcgtagc ggtagtgatg cgcgacgaac gcgccgtcgg
     1381 cgctcgcgcg gaacgtgagc gtcaggcagt cgaagggctg cgccgtgccg taccagacga
     1441 agcggttcgt gagctgccgc acctcggtcc cgaaccgatc ggcgagcgag cgccgcacca
     1501 gcgaattgaa gccgtccgcg ccgaccacga gatcggccgc gcccagctcg tccagcgacg
     1561 cgagggtcgt gtcccaccgc accgcgacgc ccgcgtcggc gcagaggcga ccgaggatct
     1621 cgagcagccg caggcgcgcg atggcggcga agccgttgcc gtcgatgtcg acgcgctcgc
     1681 cgcgatggac gatgcgctgg acgggccagg tctccatcgc cgcggacagc gcggcgagga
     1741 cgtcgggcgc gtcgcgctcg aggaaagcga gcgcgcccgc cgagaacacc acgccgaagc
     1801 cgtaggtcgc gccgaccggg ttctgctcga cgacgtcgac gctgcaggcg ggaaaccgac
     1861 gccgggcgag gagcgcgaac agcaggccgg cggggccggc gccgacgacg gcgatgcgca
     1921 cgcggcgctc aggtgaaatc gagccgggtg tcgagcagca cgccgaacag gtggatcggc
     1981 tcgcgccgcg ccatctcgag cggcggcagc ggcgcggcct cgaggtcata gccgctcgcc
     2041 cgcaagacct ccatcacgcc ttcggagaac acgaactcgc ccgagcgcgc ccggtgcagc
     2101 aggcgctcgg ccacgctcac gcagtcgccg aggacgagca aacggtcggc gagcgcgccg
     2161 tccgtgtagc cgagaaccgc atcgcccgag tgcaggccca tcgcgacggc ggccttgagg
     2221 ccgaactcgc tctgccagcg ctccgcgagc agggcgaact cgctctggat gcggcgcgcc
     2281 gcgtcgacgg cgtgcggcgc gtcgtcggcg ccggtgaagg tggccatcac ggtgtcgttg
     2341 aggaggtgca gcacgacgcc ctcgtgctgc tcgaccgcgg cggagaccag cccgaagaac
     2401 tccgagacgc gcgcgagcgc gaccgcaggg tcgagcatct ccgacatgcg ggtgaagccg
     2461 cgcgtctcgg cgaagaggat gaccgtcggc gcgcgctcgg gggcgtcggc gttctctcgg
     2521 ctcatcgtcg tcaggctccg tgtcccgctg ccaattcccg gcaaagtcta cgggtgcggc
     2581 cgcgacggcg tcaacggccg cgggccatcc cgctcccgaa atgttacgct cacgcctccg
     2641 gaggacgaac gagagaggaa cgcaagcgat gaacgcagcc ctgcttcgcc tcgcgcgcgc
     2701 gtgcatcgtc gcgctcgcgg cgctgcccgc ggcgccggcc ggcgccgacg actaccccaa
     2761 gggtcccatc aatctcgtga tcccgctcgc gcccggcgac gcgaccgaca cgtcggggcg
     2821 cggcatcgcc gaggagctcg cgcgcgagct gaaggtgccg gtcgtcccgg tcaaccgccc
     2881 cggcggtggc ggctcggtcg gcaccgagag cgtcgtcaag gcgaagcccg acggccacac
     2941 catcaccctc acgaacaacg cggcggtgat ctaccgctcg atcctcgacc cgaagaacgt
     3001 cacctacgac ccgctgcgcg acctcacccc gctcggcctg gcgatgcgat cgccgacggt
     3061 catcgcggtg cgcggcgacg cgccctacaa gacgctcgcc gagctggtcg cgtactcgaa
     3121 gcagcatccc ggcgcggtgc gcatcggcac cgccggctcg gggtcggtgg gcgagttctg
     3181 cgtccgcacc atcacctcgc tcacgggcgc cgacctgacg ctcgtgccct tcaccggcgc
     3241 ctcgcccggc gtgacggctc tgctcggcgg ccacgtcgag ggcatcgcgc tcgcgctggg
     3301 cacgatgacg ggccacttgc gcagcggcgc cttccgcggc atggtcatct cgagcaagta
     3361 cccggacttc cccggcatcc cgaccatggc cgagctcggc tacaaggagc cgctgttcga
     3421 cgtctgggtc gcgttcttcg cgcccgccgg cgtgccgccc gaggtgacga gcgtcctggt
     3481 ccccgcgctc gagcgcgcga tcaagtcgcc tccgatcgcg gccaagctca agccgctcgg
     3541 gatgattctc gactacgtgc cgccagacgc cctggtcgag cagatccgcg tcgagcaccg
     3601 tcgcgtcgag gcgatcgcga gcaaggccgg gctggtcaag tagcgcgccg cgtcagcgcg
     3661 acgacggcgc ggcccccagc cgcgcgcgcg cccactccac cgcgcggcgc gcgtcgcgcg
     3721 cctggaccgc ggccttcagg cggatccagt ccgcggtctt ct
//
LOCUS       DATDMW010000187         8625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1551035, whole genome shotgun sequence.
ACCESSION   DATDMW010000187 DATDMW010000000
VERSION     DATDMW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8625
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551035"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1950)
                     /locus_tag="VI319_08235"
     CDS             complement(4..1950)
                     /locus_tag="VI319_08235"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY6863874.1"
                     /translation="MIALRILKWAGITILALWLLLVGFITFFSFDFMRGPIERMVKKS
                     TGRELSIQGDLRLKWAWPVPHVVAEKLTFGNPPWARERNMLELDRAEATFDVTEMIKR
                     VWVIVEARVDNPVIHLQQAADGRKNWLLDTQQKNEKSRVEVGRLVVNKGKITYLDEGH
                     DTDISADVQTSAAGTGVTFQAKGRFNGLPLAASGSGGSIIALREETHPYPLDVEATIG
                     RTRARAKGMVTSLTRLTAVDAEVALQGRSLAELYDVIGIALPDTPAYSTSGHVAHKDE
                     VWRYEKFSGKIGSSDVAGTVQVDLGRERPYLSGEVASKLLSIADLGPMLGGGEPKNKA
                     PAAGRARVLPDTPFDTERWDTVDADVAFRAGTLKYVNELPLQDVDARVKMDGAVLTID
                     PLKFAAAGGKVDASIRLDAREEPLAAKLTARAQGVKVEQLLRTTKLEKAGIGRFDAAV
                     DLAGSGNSIARILGASNGKVGLVVPAGEVSAEIMAMVAIDLWRIAKFKLGGDEPVKIR
                     CGVADFEVKNGVATANALVFDTEPVNVQGEGTVDLRREQLDITLYPEPKQRSLASLRT
                     PLYVSGPFAKPKAGPDKGRLAAKGLGAVVLGAVAPLLAVLPLVDTGPGADSNCVKLVQ
                     QAKALPKEPSQAQANQRPGGTPRS"
     gene            2080..2844
                     /locus_tag="VI319_08240"
     CDS             2080..2844
                     /locus_tag="VI319_08240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6863875.1"
                     /translation="MTAPRKAALVTGGATGIGRSAVLALARAGFDVAVNYSSSEAAAR
                     DAAAEVEKAGARALLVRCDVSDENAVRAMAAKIDATFGRLDVLVNNAGTTAKWKPRDL
                     ETLSLEEWDRVFAVNVRGLFQVTRAMLPLLKKGEHPCVINTASIVGLRPGPQPLPYAA
                     SKAAVVNLTKTLAWNLGPAIRVNAVAPGWMEGDWMMRMLGDKYDELMGKRAKATPLKR
                     VVTADDVAETMMSLVLGNRFVTGEVIVIDGGFTSST"
     gene            2852..4624
                     /locus_tag="VI319_08245"
     CDS             2852..4624
                     /locus_tag="VI319_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6863876.1"
                     /translation="MLAGVVPFPPEFARRYREKGYWRDRTLAQEFAEVFRRYADRICI
                     VEGERSYTYTDVDRLSDRLALNLLHLGLRPLDRVVLALPNVGEFVILYFALQKIGCIP
                     IAALATHRYAEIGQFVRIAQAAACVYPEKQGSFEFGPMIRRVQEESRCLRFPIVLGKA
                     RYWEHSLAQLMDDAPKAPRGVLEKIAIDPVDPCIFQLSGGTTGVPKLIPRTHNDYAYN
                     SRIAAEVCEVTADTVMLIALPIAHNLPLACPGIQGTMLAGGKVVLCASTKPEAMFEAI
                     QKHRVTHVKVVPALLIRLINHPDAGEYDLSSVRIIQSGGQRMQPEVRTRTRQLIPGVF
                     VQENFGMSEGMLLFVRRGDPEDVVMETCGRPISPDDEIMLLDDDDNVVKPGDVGELCA
                     RGPYTLRGYYGAEEHNKRAFTPDGFYRSGDLMRQHPSGNYVVEGRKKDLINRGGEKIS
                     AEEIENLILSHPAVQNVACIPVPDPDLGERMCACAILRAGAALSLPELIAYLSQKEIA
                     KFKLPERLEIMDDFPLSTFGKVSKKALVETVSRKMASEPKPAPRAAAPRPQGPARELG
                     ERAWRVVVGAGRLALQTLRRRKAS"
     gene            4627..5469
                     /locus_tag="VI319_08250"
     CDS             4627..5469
                     /locus_tag="VI319_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6863877.1"
                     /translation="MRIIDLHCYPGTREWIACQGPYVEALAKYWSRAWVAKTEDEVLK
                     DFTDAGVEAVLVALDLETTIGTQPVTNEYVHAMWKRHPKRIIQAWGALEPAKGEVAIA
                     QAKKAVRELGLVGFHFHPIMQHFAVNDRRWYPLFETISELKAAVMIDIGTTGMGAGLP
                     GGMGARIRHAHPSAIDDLAADFPDMTIVMAHPGWPWVDEATAVALHKGNVYWEMSGWA
                     PKYFPQSLKVDMRARLQDKIMFGSDYPSLPYARILKEWAELGYSDAVMEKIFHGNAER
                     VLRL"
     gene            5554..6231
                     /locus_tag="VI319_08255"
     CDS             5554..6231
                     /locus_tag="VI319_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863878.1"
                     /translation="MKQAHMRSAAVIAVIAAALSGQVGAQTMYRCGKVYQDRPCDAGQ
                     QGRAVGSATTGAGGGTSDAECRQRGERALKIIWAREGGTTADAALSQAGSADDRSLVQ
                     QVYARRGSSSQIRAAIEADCVAQKERDAQAAAMVKAMGLSGSPQQPAQPAAPPTGEDP
                     AAAEARRQRLAAENDAAVKKATCSQLNTEMQRNVSAQRTGGSAATMERLNDQRRDIER
                     RTREAGC"
     gene            6239..7030
                     /locus_tag="VI319_08260"
     CDS             6239..7030
                     /locus_tag="VI319_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6863879.1"
                     /translation="MLFLYHGDTSVCASKARLVLAEKDLSWEGRLLDLRKGEQHAPNY
                     LKLNRNAVVPTLVHDGRVVVESTVIIEYLDEAFPSPPLMPADPFRRAQARLWMKKIDD
                     YLHAACSALTFAVAFRETMLKKDPKDLAAHLARIPDPAYRERQRLSIELGLDAPHVAQ
                     AVRQHERFVGEMEEALKASRWLAGDDYSLADAAATPYLHRAELLGMEGLWAARPYVAE
                     WLSCVRARRSFEDGIVKWFSDEARRRFDVPRAEIWGKVRRILGAA"
     gene            7041..8210
                     /gene="pobA"
                     /locus_tag="VI319_08265"
     CDS             7041..8210
                     /gene="pobA"
                     /locus_tag="VI319_08265"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEY6863880.1"
                     /translation="MRTQVAIIGSGPAGLFLGQLLHHAGIDAVILERKSKEYVIGRLR
                     AGLLEQGTVDMMDQVKASERLHRHGLVHEGLDLCFAGVRHRIDLYGLTGRKVTIYGQT
                     EMTRDLIEAREACGAKSVYEAEDVSVHDFDGTRPRVRWRKDGVARELECDFIAGCDGY
                     HGVCRPSVPQRAIATFERVYPFAWLGILSDTPPVSHELVYVHHERGFALCTMRSTTRS
                     RLYIQCAGDEKVEDWTDERFWSELKSRLDPKTAAEIVTGPAIETTFTALRSFVAEPMR
                     FGRLFLAGDAAHIVPPTGAKGLNLAASDVHYLSEALVEHYRTGSSAALDAYSAKALRR
                     VWQAERFSWWMTGLLHKFPETGPFGWKMQLAELDYIASSKAAAKAMADNYVGLPF"
     gene            8215..>8625
                     /locus_tag="VI319_08270"
     CDS             8215..>8625
                     /locus_tag="VI319_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863881.1"
                     /translation="MARVLEGGCHCGKVRVELTTSMRTEEFRPRACDCSFCTKHGAAY
                     VSDPAGRLRIVASGGLADYRQGSGTARMLLCRSCGVLVAAVLEEDGRRYGTVNAKCLD
                     GVAFGEPQAVSPQTLAVDDKKARWTRLWTPDVEIV"
BASE COUNT         1345 a         2973 c         3032 g         1275 t
ORIGIN      
        1 ggactagctc cgaggcgtgc cgcccggccg ctgattcgcc tgagcctgcg acggctcctt
       61 cggcagcgcc ttcgcctgct gcaccagctt gacgcaattg ctgtccgcgc cgggtcccgt
      121 gtcgaccagc ggcagcacgg cgagcagcgg cgccaccgcg ccgagcacca ccgcgccgag
      181 gcccttggcg gcgaggcgtc ccttgtccgg gccggctttg ggcttcgcga acgggccgct
      241 cacatagagc ggcgtgcgca gcgacgcgag gctcctctgc ttgggctccg ggtagagcgt
      301 gatgtcgagc tgctcgcgcc gcaggtcgac cgtgccttcg ccctggacgt tgaccggctc
      361 ggtgtcgaag acgagcgcgt tcgcggtcgc cacgccgttc ttcacctcga agtccgccac
      421 gccgcagcgg atcttgacgg gctcgtcgcc gccgagcttg aacttggcga tgcgccacag
      481 gtcgatcgcg accatcgcca tgatctcggc gctgacctcg ccggcgggca cgacgaggcc
      541 caccttgccg ttcgacgcgc ccaggatccg cgcgatcgag ttgcccgagc ccgccaggtc
      601 cacggccgcg tcgaagcggc cgatgcccgc cttctcgagt ttggtggtcc gcagcagctg
      661 ctcgaccttg acgccctgcg cgcgtgccgt gagcttcgcg gcgagcggct cctcgcgcgc
      721 gtcgaggcgt atcgacgcgt cgaccttgcc gcccgcggcg gcgaacttga gcgggtcgat
      781 ggtgagcacg gcgccgtcca tcttcacgcg cgcgtcgacg tcctgcagcg gcagctcgtt
      841 cacgtatttc agcgtcccgg cgcggaacgc cacgtcggcg tcgaccgtgt cccagcgctc
      901 ggtgtcgaac ggcgtgtcgg gcagcacgcg cgcgcgcccg gccgcgggcg ccttgttctt
      961 cggctccccg ccgccgagca tggggcccag gtcggcgatg ctgagcagct tcgacgcaac
     1021 ctcgccgctg agatacgggc gctcgcggcc gaggtcgacc tgcaccgtgc cggcgacgtc
     1081 gctggagccg atcttccccg agaatttttc gtagcgccac acctcgtcct tgtgcgcgac
     1141 gtggccggag gtcgagtacg cgggggtgtc ggggagcgcg atgccgatga cgtcgtagag
     1201 ctccgcgagg ctgcggccct gcagcgccac ctcggcgtcc accgcggtga gccgggtcag
     1261 gctcgtcacc atgcccttcg cgcgcgcccg cgtgcggccg atggtggctt cgacgtcgag
     1321 cggatacgga tgcgtttcct cgcgcagcgc gatgatcgag ccgccgctgc cgctcgccgc
     1381 gagcggcaga ccgttgaagc gacccttggc ctggaacgtg accccggtgc ccgcggcgga
     1441 cgtctgcacg tcggcgctga tgtcggtgtc gtgtccctcg tcgagatagg tgatcttgcc
     1501 cttgttcacg acgagccggc cgacctcgac gcgcgacttc tcgttcttct gctgcgtgtc
     1561 gagcagccag ttcttgcgcc cgtccgccgc ctgctgcagg tggatcaccg ggttgtcgac
     1621 gcgcgcctcg acgatgaccc acacgcgctt gatcatctcg gtcacgtcga aggtcgcctc
     1681 ggcgcggtcg agctcgagca tgttgcgctc gcgcgcccac gggggattgc cgaaggtgag
     1741 cttctcggcg acgacgtggg gcacgggcca tgcccacttg agcctcaggt cgccctggat
     1801 cgagagctcg cggccggtcg atttcttgac catgcgctcg atcggtccgc gcatgaaatc
     1861 gaagctgaag aacgtgatga agccgaccag cagcagccac agcgcgagga tggtgatccc
     1921 cgcccatttc aggattcgga gcgcgatcat ggcggcggcg ggcacacggc ttctcgcttc
     1981 atgctgcagc aggcaaatga aggaaccgag gtggccggca taggccgcct tgacacgctc
     2041 cgggaaacga ttgaaactga acatgttcgg atttctgcga tgactgcccc gaggaaagca
     2101 gccctggtca cgggcggtgc gaccggcatc gggcgcagcg cggtgctcgc gctcgcgcgc
     2161 gccgggttcg acgtcgccgt gaactacagc tcgagcgagg ccgccgcgcg cgacgccgcc
     2221 gcggaggtgg agaaggccgg cgcgcgcgcg ctcctcgtga ggtgcgacgt cagcgacgag
     2281 aacgcggtgc gggcgatggc cgccaagatc gacgcgacct tcggccgcct cgacgttctg
     2341 gtgaacaacg ccggcacgac cgcgaagtgg aagccgaggg acctcgagac gctctcgctc
     2401 gaggaatggg accgggtctt cgccgtgaac gtgcgcggcc tgttccaggt gacgcgcgcg
     2461 atgctgccgc tgctgaagaa gggcgagcac ccgtgcgtga tcaacaccgc gagcatcgtc
     2521 ggcctgcggc ccgggccgca gccgctgccg tacgcggcga gcaaggctgc ggtggtcaac
     2581 ctcaccaaga cgctcgcgtg gaatctcggc ccggcgatcc gggtcaacgc cgtcgccccc
     2641 ggctggatgg aaggcgactg gatgatgcgc atgctcggcg acaagtacga cgagctgatg
     2701 ggcaagcgcg ccaaggcgac gccgctcaag cgcgtggtca ccgctgacga cgtggccgag
     2761 acgatgatga gcctggtcct cggcaaccgc ttcgtcacgg gggaggtgat cgtgatcgac
     2821 ggcggcttta ccagctcgac ctaggcgcgc cgtgctcgcc ggcgtcgtcc cgttcccgcc
     2881 cgagttcgcg cggcgctatc gcgagaaggg ctactggcgc gaccgcacgc tcgcgcagga
     2941 gttcgccgag gtcttccgcc gctacgcgga ccgcatctgc atcgtcgaag gcgagcgcag
     3001 ctacacctac accgacgtcg accgcctgag cgaccggctc gcgctcaacc tgctgcacct
     3061 gggcctgcgg ccgctcgacc gcgtcgtgct cgcgctgccg aacgtcggcg agttcgtgat
     3121 cctctatttc gcgctgcaga agatcggctg catcccgatc gccgcgctcg cgacgcaccg
     3181 ctacgcggag atcggccagt tcgtgcgcat cgcgcaggcg gccgcgtgcg tgtacccgga
     3241 gaagcagggc agcttcgagt tcgggccgat gatccggcgc gtgcaggagg agagccgctg
     3301 cctgcgcttc ccgatcgtgc tcgggaaggc gcgctactgg gagcattcgc tcgcgcagct
     3361 gatggacgac gcgcccaagg cgcctcgcgg cgtcctcgag aagatcgcca tcgacccggt
     3421 ggacccgtgc atcttccagc tctcgggcgg caccaccggc gtgcccaagc tgatcccgcg
     3481 cacgcacaac gactacgcct acaactcgcg catcgcggcc gaggtgtgcg aggtgaccgc
     3541 cgacacggtg atgctgatcg cgctgccgat cgcgcacaac ctgccgctcg cctgccccgg
     3601 catccagggc acgatgctcg ccggcggcaa ggtcgtgctg tgcgcgagca cgaagcccga
     3661 ggcgatgttc gaggcgatcc agaagcaccg cgtcacgcac gtcaaggtcg tgcccgcgct
     3721 gctgatccgg ctcatcaacc atccggacgc cggcgaatac gacctgtcgt cggtccggat
     3781 catccagagc ggcggccagc gcatgcagcc cgaggtgcgc acacgcacgc ggcagctgat
     3841 ccccggcgtg ttcgtgcagg agaacttcgg catgtcggag gggatgctgc tgttcgtgcg
     3901 gcgcggcgat cccgaggacg tggtcatgga gacttgcggc cgcccgatct cgcccgacga
     3961 cgagatcatg ctgctcgacg acgacgacaa cgtggtgaag cccggcgacg tgggcgagct
     4021 gtgcgcgcgc gggccgtaca cgctgcgcgg ctactacggc gccgaggagc acaacaagcg
     4081 tgcgttcacg ccggacggct tctaccgctc gggcgacctc atgcgccagc acccctcggg
     4141 caactacgtc gtcgagggcc ggaagaagga tctcatcaac cgcggcggcg agaagatcag
     4201 cgccgaggag atcgagaacc tgatcctgtc gcaccccgcg gtgcagaacg tcgcgtgcat
     4261 cccggtgccc gatcccgacc tgggcgagcg catgtgtgcc tgcgcgatcc tgcgcgcggg
     4321 cgccgcgctc tcgctgcccg agctcatcgc gtacctgtcg cagaaggaga tcgcgaagtt
     4381 caagctgccc gagcgcctcg agatcatgga cgatttcccg ctctcgacct tcggcaaggt
     4441 ctcgaagaag gcgctggtcg agaccgtgtc gaggaaaatg gcgtcggagc cgaagcccgc
     4501 gccgcgggcc gcggccccgc ggccgcaagg cccggcgcgc gagctgggcg aacgcgcctg
     4561 gcgcgtggtc gtgggcgccg gccgtctcgc gctgcagacg ctgcggcggc gcaaggcctc
     4621 ctgaccatgc gcatcatcga cctgcactgc tatcccggca cccgcgaatg gatcgcgtgc
     4681 caggggcctt acgtcgaggc gctcgcgaaa tactggagcc gcgcctgggt cgcgaagacc
     4741 gaggacgagg tcctcaagga cttcaccgac gcgggcgtcg aggcggtgct cgtcgcgctc
     4801 gacctcgaga cgacgatcgg cacgcaaccc gtcacgaacg agtacgtgca cgcgatgtgg
     4861 aagcgccacc cgaagcgcat catccaggcg tggggcgcgc tcgagccggc gaagggcgag
     4921 gtcgcgatcg cgcaggcgaa gaaagcggtg cgcgagctcg ggctggtcgg cttccacttc
     4981 cacccgatca tgcagcactt cgccgtgaac gaccggcgct ggtacccgct gttcgagacg
     5041 atcagcgagc tcaaggcagc ggtcatgatc gacatcggca cgaccggcat gggcgcgggg
     5101 ctgcccggcg ggatgggagc gcgcatccgc cacgcgcatc cctcggcgat cgacgacctc
     5161 gccgccgact tcccggacat gaccatcgtc atggcgcacc cggggtggcc ctgggtcgac
     5221 gaggcgaccg cggtcgcgct gcacaagggc aacgtctact gggagatgtc gggctgggcg
     5281 ccgaaatatt tcccgcaatc gctcaaggtc gacatgcgcg cgcgcctgca ggacaagatc
     5341 atgtttggga gcgactatcc gagcctgccg tatgcgcgca tcctcaagga atgggccgag
     5401 ctcggctatt ccgacgccgt gatggagaaa atttttcacg gcaatgccga gcgcgtgctc
     5461 aggctatagc gggcgccccg ccgaatcgtc cgccggggat cgcgtcgtgt attacactga
     5521 tccggcagga cgacaaatca ggggagagca accatgaagc aggcgcacat gcgctcggcc
     5581 gccgtgatcg ccgtgatcgc cgcggcgctg tccgggcagg tcggggcgca gacgatgtac
     5641 cgctgcggca aggtgtacca ggatcgcccg tgcgacgccg ggcagcaggg ccgcgcggtg
     5701 ggaagcgcga ctaccggggc aggcggcggc acgagcgacg ccgagtgccg ccagcgcggc
     5761 gagcgcgcgc tcaagatcat ctgggcgcgc gaaggcggca ccaccgccga cgccgccctg
     5821 tcccaggcgg gaagcgcgga cgatcgcagc ctcgtgcagc aggtctatgc gcggcgcggc
     5881 tcgtcctcgc agatccgcgc ggcgatcgag gcggattgcg tcgcgcagaa ggagcgcgac
     5941 gcgcaggccg ccgccatggt gaaggccatg ggcctctccg gaagcccgca gcagccggcc
     6001 cagcccgcgg cgccgccgac gggtgaggac cccgccgcgg ccgaggcgcg gcgccagcgc
     6061 ctggcggcgg agaacgacgc ggccgtgaag aaggcgacct gctcgcagct gaacaccgag
     6121 atgcagcgca acgtctcggc gcagcgcacc ggcggcagcg cggccaccat ggagcggctg
     6181 aacgaccagc gccgcgacat cgagcgccgc acccgcgagg cgggctgctg aggccgtaat
     6241 gctgttcctg taccacggcg acacctcggt gtgcgcgagc aaggcgcgcc tggtgctcgc
     6301 cgagaaggac ctgtcgtggg aaggtcggct gctcgacctg cgcaagggcg agcagcacgc
     6361 cccgaactac ctcaagctca atcgcaacgc cgtcgttccg acgctggtgc acgacggtcg
     6421 ggtcgtcgtc gagtcgaccg tcatcatcga atacctggac gaggcgtttc cctcgccgcc
     6481 gctcatgccc gcggacccgt tccggcgcgc gcaggcgcgg ctgtggatga agaagatcga
     6541 cgattacctt cacgccgcgt gctcggcgct cacgttcgcc gtcgcgttcc gcgagaccat
     6601 gctgaagaag gacccgaagg acctggcggc gcacctggcg cggatcccgg acccggccta
     6661 ccgcgagcgc cagcgcctct cgattgagct cggactcgac gcgccgcacg tcgcgcaggc
     6721 ggtgcgccag cacgagcgct tcgtcggcga gatggaagag gcgctcaagg cgtcgcgctg
     6781 gctcgcgggc gacgactatt cgctcgccga cgcggcggcg accccgtacc tgcaccgcgc
     6841 cgagctgctc gggatggagg gcctgtgggc ggcgcgtccg tacgtcgccg aatggctctc
     6901 gtgcgtgagg gccaggcgga gcttcgagga cggcatcgtc aagtggttct ccgacgaggc
     6961 ccgcaggcgc ttcgacgtcc cgcgcgccga gatctggggc aaggtccgcc gcatcctggg
     7021 cgcggcctga gcgagctcgc gtgcgcacac aagtcgccat catcggctcg ggccccgcgg
     7081 gactcttcct gggtcagctg ctgcatcacg ccggcatcga cgcggtcatc ctggagcgca
     7141 agagcaagga gtacgtcatc gggcgtctgc gcgcgggcct gctcgagcag ggcacggtcg
     7201 acatgatgga ccaggtgaag gcctcggagc ggctgcaccg gcacggtctc gtgcacgaag
     7261 ggctggacct gtgcttcgcc ggcgtgcgcc accgcatcga tctgtacggg ctcaccggcc
     7321 gcaaggtcac catctacggc cagaccgaga tgacgcgcga cctgatcgag gcgcgcgagg
     7381 cctgcggcgc gaagagcgtg tacgaggccg aggacgtgag cgtgcacgac ttcgacggca
     7441 cgcgcccgcg ggtgcgctgg cgcaaggacg gcgtcgcccg cgagctcgag tgcgacttca
     7501 tcgccggctg cgacggctac cacggcgtgt gccggccgag cgtcccgcag cgggcgatcg
     7561 ccaccttcga gcgcgtgtac ccgttcgcct ggctcggcat cctgtccgat acgccgccgg
     7621 tgtcgcacga gctggtctac gtccaccacg agcgcggctt cgcgttgtgc accatgcgct
     7681 cgaccacccg cagccgcctc tacatccagt gcgccggcga cgagaaggtc gaagactgga
     7741 ccgacgagcg cttctggagc gagctgaaga gccgcctcga tccgaagacg gccgcggaga
     7801 tcgtaaccgg ccccgcgatc gagacgacgt tcacggcgct gcgcagcttc gtcgccgagc
     7861 cgatgcgctt cgggcgcctg ttcctcgcgg gcgacgcggc gcacatcgtg ccgccgaccg
     7921 gcgccaaggg cctgaacctc gcggcgagcg acgtgcatta cctgtcggag gcgctcgtcg
     7981 agcactaccg caccggatcg agcgccgcgc tcgacgccta ctcggcgaag gcgctgcggc
     8041 gcgtgtggca agcggagcgc ttctcgtggt ggatgaccgg cctgttgcac aagtttcccg
     8101 agaccgggcc gttcggctgg aagatgcagc tcgccgagct cgattacatc gccagctcga
     8161 aggcggcggc gaaggcaatg gccgacaatt acgtcggcct gccgttctag tccgatggcg
     8221 cgcgtcctcg aaggcggctg ccactgcggc aaggtgcgcg tcgagctcac gacctcgatg
     8281 cggaccgagg aattccgccc gcgcgcgtgc gactgctctt tctgcaccaa gcatggggcg
     8341 gcctacgtct cggatcccgc gggccggctg cgcatcgtcg cttccggcgg gctcgccgat
     8401 taccggcagg gatcggggac tgcgcgcatg ctgctttgtc gcagctgtgg tgtcctcgtg
     8461 gcagcagtgc tggaagaaga cggacgccgc tacggcaccg tcaacgcgaa gtgcctcgac
     8521 ggcgtcgcct tcggcgagcc gcaggcggtc tctccgcaga ccctggcggt cgacgacaag
     8581 aaagcgcgct ggacgcgcct gtggacgccc gacgtggaga tcgtc
//
LOCUS       DATDMW010000188         5244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_679251, whole genome shotgun sequence.
ACCESSION   DATDMW010000188 DATDMW010000000
VERSION     DATDMW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679251"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VI319_08275"
     CDS             complement(<1..332)
                     /locus_tag="VI319_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY6863882.1"
                     /translation="MSDATEKAKELTLLDGGIEAFPRMVLAIEQARETVQLEMYLFRL
                     DATGRCFVAALSAAAGRGVQVRVSLDGWGSSQDAREIEERLAAAGCEVTIHAWRWWFL
                     RRALARNHR"
     gene            complement(341..1465)
                     /locus_tag="VI319_08280"
     CDS             complement(341..1465)
                     /locus_tag="VI319_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6863883.1"
                     /translation="MKRPELWPQLRQLTEISRALTYAASLEEASQLAVDRAAELIGAE
                     TALLLIKGEAGPLTVRASRGVDAAVAQGFAVLLSEAVVAQLAAALGVEAANVLALPLV
                     VNGEIDGLLVVVRPRGAPASERDEWLVGAIADQAAVALEKTRMVETAGFREQLMGIVG
                     HDLRNPLQTISVASHLLLLRDGMGAKETELVRKIAAAGASASKVVEQLLDLTRSRLGG
                     GIPLDVRRVDLGEICRQLIGETELKHLDRPLRVQLEGDLAGTWDRDRLYQLLANLIGN
                     AVQHGAAQSPIDLRAEGGRDEVVIAVGSRGAPIPPAMLPVIFNAFRKNRPPQPGGTPT
                     AGLGLGLFIAQQIARAHRGSIAVTSSESAGTVFRVRLPRS"
     gene            complement(1462..2868)
                     /locus_tag="VI319_08285"
     CDS             complement(1462..2868)
                     /locus_tag="VI319_08285"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY6863884.1"
                     /translation="MSNPAATPVVRISSGSREVDEVLGGGLPENSINIVMGEPGSGKT
                     TLAEAFLFANANDAKRPVLFLTTLSEPLEKVIRYGQQYRFFDADKIGTAVLYDSIAAD
                     MVTGGVSTLVPKLKEAIKAHSPRLIVIDSFKVIHDLAKSPEEVRLMLHEAAGLLTAYN
                     VTVLLVGEYRHEDIARYPEFAVADGIIELARNALGSRDERYLRVFKLRGSSYREGSHA
                     FEISSDGLQVYPRLVSPVEAASYQRSSKRASSGVPGLDAMFAGGVWQGSATLVAGPTG
                     SGKTTMGIQFVVEGLKQGERCLLVNFQENPTQLAHQLEQIGGKLDAETRERLQLQYHS
                     AVELPIDRIVVSIFKALRRSPIHRVVIDAVGDLAVAANDAARLHDYLYAMGQHFAVMG
                     VSSLLTLEVDAGVEATSAIQGRLSHMTDNIVYLTLESRSGTMARSIRVAKARGLGHDL
                     QPSGLQIDARGLQVIERS"
     gene            complement(2865..3254)
                     /locus_tag="VI319_08290"
     CDS             complement(2865..3254)
                     /locus_tag="VI319_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6863885.1"
                     /translation="MEKRGKAIILVVDRDPHVRELEAHFLGQAGYDVIFEQDGNGALE
                     RARELSPDIIVTEILVPGLDGLALCRQLKAGPKTRGIAVLVFSVLATAGRAAEAGADA
                     FLQKPLTDHALVETVARLLASAKKAGT"
     gene            3543..3896
                     /locus_tag="VI319_08295"
     CDS             3543..3896
                     /locus_tag="VI319_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6863886.1"
                     /translation="MLIVDDDADLRGAVTRLLQLEGHAVIEAADGQLALDALSAGPRV
                     GLIILDLMMPVMDGATFLAHKAKGDHAAVPVVVFSSSPGQDFAAFADVIAMVPKLEGT
                     VGLLAALQLAAFNRP"
     gene            complement(4142..4861)
                     /locus_tag="VI319_08300"
     CDS             complement(4142..4861)
                     /locus_tag="VI319_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863887.1"
                     /translation="MRTISWKLPIVLSASFVFAADGHAASGYKGIPWGAACSEAVRNL
                     QAQSFVFDRTDKLGLPPQARSPQEPQLFARSSGLASCSSEESDLRLDTHRANPYVEIA
                     AASGDITVTLLCRDQRFVGARLETPMGRTAAVAMLTKAAGASVRTIRANTCGEEALNC
                     TADHSLLLSRGDTVRYLEKPVRSSRGYAGQDPPLIRYLILARSEDRALQTASLACAKK
                     RLADGRLEKQRIQDGNRAALQ"
     gene            complement(5046..>5244)
                     /locus_tag="VI319_08305"
     CDS             complement(5046..>5244)
                     /locus_tag="VI319_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6863888.1"
                     /translation="HRVERSYGNFGRRFTLPSTVDTGNVTAQSRDGVLRIFLPKKAET
                     KPRQVKVQTDSNLGTQAKQKQ"
BASE COUNT          823 a         1837 c         1733 g          851 t
ORIGIN      
        1 cgatgattgc gcgccagcgc ccggcgcagg aaccaccagc gccatgcgtg aatggtgacc
       61 tcgcagccgg cagcggccag tcgttcctcg atctctcgcg cgtcctgcga cgagccccat
      121 ccgtcgagcg agacgcgaac ctggacaccg cggcccgccg ctgccgacaa cgcggcgacg
      181 aagcaccggc ctgtcgcgtc gaggcggaag agatacatct ccaactgcac ggtctcgcga
      241 gcctgctcga tggccagcac catccgcgga aaggcctcga tgccaccgtc gagcagcgtc
      301 agctctttcg ccttctcggt cgcgtcgctc aagcgagacc tcaactccgc ggcagccgca
      361 cccggaagac ggttcctgcg ctctccgacg aggtgacggc gatcgagccg cgatgcgcgc
      421 gcgcgatctg ttgggcgatg aacagcccca acccgagccc cgccgtcgga gtgccgccag
      481 gctgtggcgg gcgattcttc cggaacgcat tgaagatcac cggcagcatc gcggggggga
      541 tcggcgcgcc gcggctccca accgcgatca ccacttcgtc gcgcccgccc tcggcgcgca
      601 ggtcgatcgg cgactgcgcg gcgccgtgtt gaacggcgtt gccgatcagg ttcgcgagca
      661 gctggtacag gcgatcgcga tcccacgtgc cggcgaggtc gccctcgagc tgcacccgca
      721 gcggtcggtc caggtgcttg agctccgtct ctccgatcag ctgccggcag atctcgccga
      781 ggtcgacgcg ccgaacgtcg agcggaatgc ctccgccgag ccggctgcgc gtcaggtcga
      841 gcagctgctc gacgaccttc gaggccgagg ctccggcagc ggcgatcttc ctcaccagct
      901 cggtctcctt cgcgcccatc ccatcgcgca acagtagcag gtgcgacgct actgagatcg
      961 tctgcagcgg gttcctcaga tcgtgcccga cgatccccat cagctgctcg cgaaagccgg
     1021 ccgtctccac catccgcgtc ttctcgagcg cgacggccgc ctggtccgcg atcgcgccga
     1081 ccagccattc gtcccgctcc gaggccggcg cgccgcgcgg gcgcaccacc accaagaggc
     1141 cgtcgatctc tccattcacc accagcggca gcgcgagcac gttcgccgcc tcgacgccca
     1201 gagcggcggc gagctgcgcc accaccgcct cgctcagcag aacagcgaag ccttgcgcca
     1261 ccgcggcgtc gacgccacgc gaggcgcgca ccgtgagcgg cccggcctcg cccttgatca
     1321 gcagcagcgc cgtctcggcc ccgatcagct cagcggcccg gtcaaccgcg agctgcgacg
     1381 cctcttcgag cgaggcggcg taggtcagcg cgcggctgat ctcggtcagc tgccgcagct
     1441 gcggccagag ttcgggccgc ttcacgagcg ctcgatgact tgcagcccgc gtgcgtcgat
     1501 ctgcagtccg ctcggctgga ggtcgtgacc caatccccgc gccttcgcga cgcgaatcga
     1561 gcgcgccatc gttcccgacc gcgactcgag ggtgaggtag acgatgttgt cggtcatgtg
     1621 gctcaggcgg ccctggatgg cggacgtcgc ctccaccccg gcatcgacct ccagcgtcaa
     1681 caggctgctg acccccatca ccgcgaagtg ctgtcccatc gcgtacaggt agtcgtgcag
     1741 ccgtgcggcg tcattggcgg cgacggcgag gtctccgacc gcgtcgatga cgacgcggtg
     1801 gatcgggctg cggcgcagcg ccttgaagat gctgaccacg atgcggtcga tcggcagctc
     1861 gacggcggag tggtactgca gctggaggcg ctcgcgcgtc tcggcgtcga gcttgccgcc
     1921 gatctgctcg agctggtgcg cgagctgggt ggggttctcc tgaaagttca ccagcaggca
     1981 gcgctcgccc tgcttcagcc cttcgaccac gaactggatg cccatcgtcg tcttgcccga
     2041 gcccgtgggc ccggcgacca gcgtggcgct gccctgccac acgccgcctg cgaacatggc
     2101 gtcgagcccc ggcacccccg acgacgcgcg cttcgagctg cgctggtacg aggccgcttc
     2161 gaccgggctc acgaggcgcg ggtacacttg caggccgtcc gagctgatct cgaacgcgtg
     2221 cgacccctcg cggtagctgc tgccgcgcag cttgaacacg cgcaggtagc gctcgtcgcg
     2281 ggaacccagt gcattgcgcg ccagttcgat gatgccgtcg gccacggcga actcggggta
     2341 gcgcgcgatg tcttcgtgcc ggtactcgcc gaccagcagc acggtgacgt tgtaggcggt
     2401 gagcagcccg gccgcctcgt gcagcatcag gcgcacttct tcgggcgact tcgccaggtc
     2461 gtgaatcacc ttgaacgagt cgatgacgat caaccgcggc gaatgcgcct tgatcgcctc
     2521 cttgagcttc ggcaccagcg tgctgacgcc gccggtgacc atgtcggcgg cgatgctgtc
     2581 gtacaggacg gcggtgccga tcttgtcggc gtcgaaaaag cggtactgct gcccgtaccg
     2641 gatcaccttc tcgagcggct cggagagcgt ggtgaggaag agcaccggcc gttttgcgtc
     2701 gttcgcgttc gcgaacagga aggcctcggc cagggtggtc ttccccgagc ccggctcgcc
     2761 catcacgatg ttgatcgagt tctcggggag cccgcccccc agcacctcgt cgacctctcg
     2821 gctgccggag gagatgcgca cgacgggagt ggcagcgggg ttgctcatgt tccagccttc
     2881 ttcgcgctgg cgaggagccg ggcgacggtt tcgaccagcg cgtgatcggt aaggggcttc
     2941 tgcagaaagg cgtcggcgcc ggcctcggcg gcgcggccgg cggtggcgag cacgctgaac
     3001 acgagcaccg cgatgccgcg ggtcttcggc ccggccttca gctgccggca cagcgcgagg
     3061 ccgtcgagcc ccggcacgag gatctcggtg acgatgatgt cgggagaaag ctcgcgcgcg
     3121 cgctcgagcg ctccgttgcc gtcctgctcg aagatgacgt cgtaacccgc ctggccgagg
     3181 aagtgcgcct cgagctcgcg aacgtggggg tcgcgatcga cgacgaggat gatggccttg
     3241 ccgcgctttt ccaaggctgg ctcgtatcac gttctcaccc ggaccgggat cctccgggac
     3301 ccgcgcgacg cctaatcaac atcatgtcca gcagccgcct gcacgagtgt ccggttcacc
     3361 gcgcggacga ggcgtcaagc gcgaaggtcg ccagccgcgc cagtccacac caagggtccg
     3421 aatgtggacg ccggcctcgc tcgcgccgag cgcagcctgg ccggtccagc acaatagatg
     3481 tgcgtggagc gagctttggc ctcgcggact cgtacactgc aacgatggtt gcccctgcgc
     3541 gcgtgctcat cgtcgatgac gacgccgacc tgcgcggcgc ggtaacccgc ctgctccaac
     3601 tcgagggcca cgcggtcatc gaggcggccg acgggcaact tgcgctcgac gcgctctcgg
     3661 ccgggcctcg cgtcggcctg atcatcctcg atctgatgat gccggtgatg gacggtgcaa
     3721 ccttcctcgc gcacaaggcg aagggcgacc atgccgccgt cccggtggtg gtcttttcct
     3781 cctccccagg tcaggacttc gcggcgttcg ccgatgtaat cgcgatggtc cccaagctcg
     3841 aaggcaccgt cgggctgctc gccgccctcc aactcgcggc gttcaatcga ccgtaacctt
     3901 tcccggacga gattcacggt cgcagggact cgtgagaggg ctagtgcgcc ggctccacga
     3961 ctgaccgcca atagtggcgc aagcacgccg ggccgctgcg gtccacttcg ggcgcggcga
     4021 ccatgcattc ctcgccgggg tcgcgctggt acagccgccg cgggagggcc atgtaaaagc
     4081 gcgattcgcc cgcggccgtg cggtaccggc ccaaccggtc tgcactcacg aagacccgct
     4141 tctactgcag tgccgcccgg ttcccgtcct ggatccgctg tttttccagc cggccgtcgg
     4201 cgaggcgctt cttcgcgcag gcgagcgacg cagtctgaag cgcgcggtcc tcggaccggg
     4261 cgaggatcaa gtatcgaatc aacggcgggt cctggccggc gtaaccgcgc gacgatcgga
     4321 ccggcttttc caggtagcgg acggtatctc cgcgcgaaag aaggagcgaa tggtcggccg
     4381 tgcaattcaa ggcttcctcg ccgcaggtgt tcgcgcggat ggtccgcacc gatgcaccgg
     4441 ccgccttggt caacatcgcc actgccgcgg tcctgcccat cggcgtctcg agccgcgcgc
     4501 ccacgaacct ctggtcgcgg cagagcaacg tgacggtgat gtctccggac gcggcggcga
     4561 tttctacgta cggattcgcc cgatgcgtat ccagcctcag atccgactct tcgctggagc
     4621 aggaggccag tccggagctg cgcgcgaaca gctggggctc ttgcggcgag cgagcctgag
     4681 gcggcaaccc gagcttgtca gtacgatcga agacgaagct ttgcgcctgc agattccgga
     4741 cggcctcgct gcacgccgct ccccaaggga ttcccttgta tcccgaggcc gcgtggccat
     4801 cggcagcaaa cacgaaagat gcactcaaga cgatcgggag cttccacgaa atggtgcgca
     4861 tggggtcctt atctgtccct ctgcgccgag cggcgcgaga ccgtccatca agtcgcggaa
     4921 tgggcaggcg atgagcgaga cggaacaggt ctcagtctag gggcacgaag cgcaaaagct
     4981 cgtcaatcgc ccttcccgcc gagcgaaaga aaagcggggc cgcaggggcc ccgccacctt
     5041 cacgcttact gcttctgctt cgcctgcgtg ccgagattcg agtcggtctg caccttgacc
     5101 tgccgcggct tcgtctcggc cttcttggga aggaagatgc ggagcacgcc gtcgcgactt
     5161 tgcgccgtga cgttcccggt atccaccgtc gacggcagcg tgaatctcct gccgaagttg
     5221 ccgtaggatc gctcgacgcg gtga
//
LOCUS       DATDMW010000189         3682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_928761, whole genome shotgun sequence.
ACCESSION   DATDMW010000189 DATDMW010000000
VERSION     DATDMW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928761"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VI319_08310"
     CDS             <1..463
                     /locus_tag="VI319_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863889.1"
                     /translation="EPQRAATPTQNTESWVASRAPRAGAGGAAAATPRGAAAAEEPFA
                     AGKPGGPAVAARDADAAPALDPARAFELQIAARLWGAPRRWDPRRVHARERGGRVWIW
                     IRDAEFDDAQTGAVASALSTLAREAGLELEGITVNGKVVFGRLPDDRENTT"
     gene            480..893
                     /locus_tag="VI319_08315"
     CDS             480..893
                     /locus_tag="VI319_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEY6863890.1"
                     /translation="MAVEALGNINTAATTTVPQSVVNQDDFLKILLTQLQFQDPLKPL
                     DNQEFIAQLAQFTNLEQTRELDDNVSTLLSIQSISQSIGLIGKTVDVAAQDGSQSTGT
                     VTTLGFNAGQPRLTVRTSNGAFITDVSLDQVSLVR"
     gene            915..2159
                     /locus_tag="VI319_08320"
     CDS             915..2159
                     /locus_tag="VI319_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEY6863891.1"
                     /translation="MLDTIYIGLTGLTGFSDGLKNISNNVANVNTPGFKGTQLKFEDL
                     FYQLQSGTGNGSDQSGMFMGAGMQAGGSTITFKQGDVKSTGNDLDAAVSGNGFFVIKA
                     ADGTTSYTRDGEFSIDSSGFLVTRGGTSRVQGIENGNLVDINIADLRVSPARATATIK
                     FTGNLSVDDSDNTQRVSGIPVFSADGGNNPLTITFTKDTSIGAAPRTWKITIDDAAGN
                     LVDDTGSIVFNGDGSPQAGTETHSFVFTPVGGQPMTLTLDFGTAGSFSGATNFSAGAD
                     STLALSSQDGLAAGALTKTTFDADGKLALTYSNGRKNSHGQLALAWFDFLQGLQPAGK
                     ASFDNNTNLPIRLGAANTSLFGGIQAGSIESSNVDLSQEFTDLIVIQRGFQGSSQVVT
                     VANEMMQQLLDIQNGGGGSSGG"
     gene            2156..2923
                     /locus_tag="VI319_08325"
     CDS             2156..2923
                     /locus_tag="VI319_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HEY6863892.1"
                     /translation="MKPRAFRLLGASELERVVAASGEALARWCSAWFGPGAANIVDAR
                     SAAGARPLAVAEAPVARAADYARWAAMLAGPGAYRAAATRFAAQAGDAVPARGELPPV
                     LRGAIDECFATLADELLALVRAQAVAGSAADVDSAWAPGSGAAVVTARLDGETFGALL
                     GPGVVSALAGAIRPSPRGGLARRMDCIQGRGVPVHVWAGGAELGLGSLASITPGDVIV
                     LDARIDETFALTGPGGTPLGRGFLGVRAASKALQLVS"
     gene            2976..3281
                     /locus_tag="VI319_08330"
     CDS             2976..3281
                     /locus_tag="VI319_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEY6863893.1"
                     /translation="MQVRLQEIQPHDPGGKSLAAAGNIDLIKDVRVKVTVSVGQAELT
                     VAELFALKDDSVVELQASTTDPVEILLDGKVIARGTLVAVGDRFGVGITEILPASGR"
     gene            3281..>3682
                     /locus_tag="VI319_08335"
     CDS             3281..>3682
                     /locus_tag="VI319_08335"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY6863894.1"
                     /translation="MLRALLASGIVAAACVMVTPGVAADAPMPAAPDSAIPFKKAERE
                     PSSEWVQAFGALVLVGALAWAAVYALKRSRLAPAALRGARRIRLVETARLDTRVTLYL
                     VEGEDRSYLLGRCGDSLVVIRDFEGRPGAASE"
BASE COUNT          583 a         1318 c         1268 g          513 t
ORIGIN      
        1 cgagccgcag cgcgccgcga cgccgacgca gaacaccgag tcgtgggtcg catcgcgggc
       61 gccgcgcgcc ggcgcagggg gcgcagcggc ggcgacgccg cgcggcgccg ccgcggccga
      121 ggagcccttc gccgcgggca agccgggcgg gccggccgtt gccgcgcgcg acgccgatgc
      181 ggcgcccgcg ctcgatcccg cgcgcgcctt cgagctgcag atcgcggcgc gtttgtgggg
      241 cgccccgcgc cgctgggatc cgcgccgggt acacgcgcgc gagcgcggcg ggcgggtctg
      301 gatctggata cgcgacgcgg agttcgacga cgcgcagacg ggcgccgtcg cgagcgcctt
      361 gtcgaccctc gcacgagaag ccgggctcga gctcgagggg atcaccgtca acggaaaggt
      421 cgtcttcggg cgcttgcccg acgaccgtga aaacacaacc tgacctccaa ggaggaacca
      481 tggccgtcga agcgctgggc aacatcaaca ccgcggcaac caccaccgtc ccgcagtcgg
      541 tggtgaacca ggacgacttc ctcaagatcc tgctcacgca gctgcagttc caggatccgc
      601 tcaagccgct cgacaaccag gagttcatcg cacagctcgc gcagttcacg aacctggagc
      661 agacgcgcga gctcgacgac aacgtcagca cgctgctcag catccagtcg atcagccagt
      721 cgatcggcct catcggcaag acggtcgacg tggcggcaca ggacggcagc cagtccaccg
      781 gcaccgtcac cacgctcggc ttcaacgccg gccagccccg gcttacggtg cggacctcga
      841 acggcgcgtt catcaccgac gtcagcctcg accaggtctc gctggtgcgc tgagcgccca
      901 ctgaaggaga tcccatgctc gataccatct atatcggcct cacgggcctg accggcttct
      961 ccgacgggct gaagaacatc agcaataacg tcgcgaacgt caacacgccg ggcttcaagg
     1021 gcacccagct gaaattcgag gacctgttct accagctgca gtcgggcacg gggaacggct
     1081 cggaccagtc cggcatgttc atgggcgcag gcatgcaggc gggcggcagc accatcacgt
     1141 tcaagcaggg cgacgtgaaa tccaccggca acgacctcga cgccgccgtg agcggcaacg
     1201 gcttcttcgt gatcaaggcc gcggacggga cgacgagcta cacccgcgac ggcgagttca
     1261 gcatcgactc tagcgggttc ctcgtgacgc gcggcgggac ctcgcgcgtc cagggcatcg
     1321 agaacggcaa cctggtcgat atcaacatcg ccgatctgcg ggtcagcccg gccagggcca
     1381 cggcgacgat caagttcacc ggcaacctgt cggtcgacga ttccgacaac acccagcgcg
     1441 tgagcggcat ccccgtgttc agtgcggacg gcggcaacaa tccgctcacg atcaccttca
     1501 ccaaggacac gagcatcggc gcggcgccgc gtacctggaa gatcaccatc gacgacgccg
     1561 ccggcaacct ggtcgacgac acgggctcca tcgtcttcaa cggcgacggc tcgccgcagg
     1621 ccgggaccga gacgcacagc ttcgtgttca ccccggtggg cgggcagccg atgacgctga
     1681 ccctcgattt cggcacggcc ggcagcttct ccggcgcgac caatttctcc gccggggcgg
     1741 actcgacgct cgcgctctcg tcgcaggatg ggctcgccgc gggcgcgctc accaagacca
     1801 cgttcgacgc cgacggcaag ctcgcgctca cgtattcgaa cgggcggaaa aactcccacg
     1861 gccagctcgc gctggcgtgg ttcgacttcc tccagggatt gcagcccgcc ggcaaggcct
     1921 ccttcgacaa caacaccaac ctcccgatcc ggctcggcgc ggccaacacc tcgctcttcg
     1981 gcggaatcca ggccgggagc atcgagtcgt cgaacgtgga cctgtcgcag gagttcaccg
     2041 atctcatcgt catccagcgc ggcttccagg gttcgtccca ggtggtgacg gtcgccaacg
     2101 agatgatgca gcagctgctc gacatccaga acggcggcgg cgggagcagc ggcggatgaa
     2161 gccgcgcgcg ttccgtctgc tcggggcgag cgagctcgag cgcgtcgtgg ccgcctccgg
     2221 cgaggcgctc gcacgctggt gcagcgcctg gttcggaccg ggcgcggcaa acatcgtcga
     2281 cgcgcggtcg gccgccggcg cgcggcccct tgccgtcgcc gaggcgccgg tcgcccgcgc
     2341 ggccgactac gcgcgctggg ccgcgatgct cgcgggcccg ggcgcgtacc gcgccgcggc
     2401 cacgcgcttc gcggcgcagg cgggcgacgc ggtgcccgcg cgcggcgagc tgccgccggt
     2461 gctgcgcggg gcgatcgacg aatgcttcgc gacgctcgcg gacgagcttc tcgccctcgt
     2521 gcgcgcgcag gccgtcgctg gatccgcagc cgacgtcgat tccgcctggg cgcccggctc
     2581 gggcgcggcg gtcgtgacgg cgcgcctcga cggcgagacg ttcggcgcgc tgctgggccc
     2641 gggcgtcgtg tcggcgctgg ccggcgcgat ccgaccttct ccccgcggcg gtctcgcccg
     2701 ccgcatggat tgcatacagg gccggggcgt gccggtgcac gtatgggcgg gcggcgccga
     2761 gctcggactc ggctcgctcg catcgatcac gccgggcgac gtcatcgtgc tcgacgcacg
     2821 catcgacgaa accttcgccc tgacgggtcc gggcggcacg ccgctcggac gcggtttcct
     2881 gggcgttcgc gccgcatcga aggcgctgca gctcgtttct tgaaagcagg gactcaatga
     2941 tcaccgaaaa atccgaaaca cacgaatcct cagccatgca ggtccgcctc caggagatcc
     3001 agccgcacga tccgggcggc aaaagcctcg cggcggcggg caacatcgac ctcatcaagg
     3061 acgtcagggt gaaggtcacc gtttccgtcg gccaggccga gctcacggtc gccgagctgt
     3121 tcgcgctcaa ggacgactcc gtggtcgagc tgcaggcgtc caccaccgac ccggtcgaga
     3181 tcctgctcga cggcaaagtg atcgcgcgcg gtacgctggt cgcggtgggc gaccgcttcg
     3241 gcgtcggcat caccgagatc ctgccggcgt cggggcgcta gtgctacgcg cgctcctcgc
     3301 ctcgggcatc gtcgcggccg cctgcgtcat ggttacgccc ggcgtcgccg cagacgcgcc
     3361 gatgcctgcg gcgcccgaca gcgcaattcc gttcaagaag gccgagcgcg agccttcgtc
     3421 cgagtgggtg caggccttcg gcgcgcttgt tctcgtgggt gccctggcgt gggcagcggt
     3481 gtacgcgctc aagcgctcgc gcctcgcgcc agcggcgctg cgcggcgcgc gcaggatccg
     3541 gctggtggaa acggcgcgcc tcgacacgcg cgtcacgctg tacctggtgg aaggggagga
     3601 ccgttcctac ctgctcgggc gctgcggcga ctcgctcgtc gtgatcaggg acttcgaggg
     3661 gcgcccgggc gcggcctcgg ag
//
LOCUS       DATDMW010000190         8225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1178057, whole genome shotgun sequence.
ACCESSION   DATDMW010000190 DATDMW010000000
VERSION     DATDMW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178057"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VI319_08340"
     CDS             <1..1231
                     /locus_tag="VI319_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY6863895.1"
                     /translation="EICHGCRRCVNLCTTFPTLFDLVDESATMEVDGVEKPKYWNVVD
                     QCYLCDLCYMTKCPYVPPHPWNVDFPHVMLRAKALKFAKGGTRFRDRLLSSTDAMGKL
                     AAIPVVAQAVNAANASPPVRAVMEKALGVHRDRELPPFAPRTFRGKAKASASREPRAG
                     ERTPGKAAIFATCYVNYNEPGIGHDLLAVLDHNDIPYVIVDKEACCGMPKLEIGDLES
                     VARLKEINVPRLARLAREGYAILTPVPSCTLMFKTELRLLFPEDAETKQVADAMFDPF
                     EYFVLRHKDGLLRTDFRKPLGRLSYHIPCHSRVQNMGQKTREALALVPGTEVRVVERC
                     SGHDGTWGVKSEHFANSMKIGRPVFRLMAEGDPDWISSDCPIAARQIEQGMAEGGKDV
                     RAKKAHPLTLLRMAYGL"
     gene            1233..1814
                     /locus_tag="VI319_08345"
     CDS             1233..1814
                     /locus_tag="VI319_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY6863896.1"
                     /translation="MPRIERASLLTLEAYARERGAFRARVIEHKRHRTVALGEHVRLI
                     FEDELTIRYQVQEMLRVERIFEESGIQEELDTYNPLVPDGRNFKATLMLEYPDPEERQ
                     RELARLKGVERRIWLEVEGSPRIWAIADEDLERENEEKTSSVHFLRFELDERAAQAAK
                     RGAALSLGVDHPQCRAAVALPGAVRDAVVQDLA"
     gene            1811..2353
                     /locus_tag="VI319_08350"
     CDS             1811..2353
                     /locus_tag="VI319_08350"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEY6863897.1"
                     /translation="MRLPSLAAASLLAALCAGAFAQVLAPQYPGGYKPQFDEDKPWEE
                     QKWALPAYPADENLLRFYVSPTTPFEFFIDSKSVSVGKDGVVRYTLVARSPSGTSNVS
                     YEGIRCESRERRLYAFGGADRTWLQARSADWARIPSRRSFPNNQQAALADEFFCVQGS
                     HVGSPDVAVNALARASRELR"
     gene            complement(2358..3464)
                     /gene="proB"
                     /locus_tag="VI319_08355"
     CDS             complement(2358..3464)
                     /gene="proB"
                     /locus_tag="VI319_08355"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY6863898.1"
                     /translation="MKAARRFVVKVGSSLVTNHGQGLDAAAIGRWAAQVAELRGAGHE
                     VILVSSGAIAEGMQRLGWSRRPRTMHELQAAAAVGQMGLAQVYESCFRGHGLRTAQVL
                     LTHADLADRQRYLNARSTLLTLLSLGIVPVINETDTVVTDEIRFGDNDTLGALVTNLI
                     EADIFVILTDQEGLYTSDPRRDAAATLVRSANAGDPRLEDMAGGAGSETARGGMLTKV
                     LAAKRAARSGAQTVIASGRESDVLRRIAAGEQIGTLIQPQTTALAARKQWLADHVQIG
                     GRLHLDAGAARALLARGKSLLPVGVKSVEGDFERGAVIACVDPDGREIARGLVNYSAQ
                     ESRRIAGRASSEIEAILGYVDEPELIHVDNLVLL"
     gene            complement(3461..4480)
                     /gene="obgE"
                     /locus_tag="VI319_08360"
     CDS             complement(3461..4480)
                     /gene="obgE"
                     /locus_tag="VI319_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY6863899.1"
                     /translation="MKFIDEAVIEVHAGKGGNGVVSFRREKYVPRGGPDGGDGGRGGS
                     VWAVADGNINTLVDYRFARIHRAKDGERGQGGQKNGRGADDVVLRMPVGTVIADAETG
                     QAICDLDKDGERALLARGGEGGLGNVNFKSSINRAPRKATPGGEGENRKLKLELKVLA
                     DVGLLGLPNAGKSTLIRAVSAARPKVADYPFTTLQPYLGVVRLGVDRSFVIADIPGLI
                     EGAAEGAGLGHQFLRHLARTRLLLHVIDVAPLEPGADPARDARAIAAELKKYDPGLHR
                     KARWLVLNKMDMLPAEEREPAAAAIVRKLRWRGPHFGVSALTGAGCTELSSAVMHFLE
                     TGKTR"
     gene            complement(4547..4804)
                     /gene="rpmA"
                     /locus_tag="VI319_08365"
     CDS             complement(4547..4804)
                     /gene="rpmA"
                     /locus_tag="VI319_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY6863900.1"
                     /translation="MAHKKAGGSSRNGRDSESKRLGVKRYGDQIVLAGSILVRQRGTR
                     IHAGENVGMGKDHTLFAKVDGKVKFAVRGPSRRQTVSVVPA"
     gene            complement(4814..5125)
                     /gene="rplU"
                     /locus_tag="VI319_08370"
     CDS             complement(4814..5125)
                     /gene="rplU"
                     /locus_tag="VI319_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY6863901.1"
                     /translation="MYAVVKTGGKQYRVSAGEKLRVEKLAAEVGAEVVLDQVLAVGEG
                     ESLKIGTPVVPGAKVKAKVVSHVLGEKLKIFKLRRRKSSKRTTGHRQRYTEIEITGIS
                     G"
     gene            5278..6246
                     /gene="ispB"
                     /locus_tag="VI319_08375"
     CDS             5278..6246
                     /gene="ispB"
                     /locus_tag="VI319_08375"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEY6863902.1"
                     /translation="MPLESIQKLIAADMQAVDALIRARLDSDVVLIRTIAEYIIGGGG
                     KRLRPALVLLASGALGYRGRDHHELAAVIEFIHTATLLHDDVVDESELRRGRKTANAE
                     FGNAASVLVGDFLYSRAFQMMVALSNMRVMEVLADATNTIAEGEVLQLLNSHNTRVSE
                     QQYLEVIRRKTAKLFEAAAQLGGILGGAGSAAESGLARYGMHLGTAFQLIDDVLDYSG
                     DAAHTGKNLGDDLNEGKPTLPLIYVMERGAEAQARTVRQAIEHGGRGEFGDVLAAIQS
                     TGALDYARAKATEEAKTARDALAPLADSKHKECLLELATFAVERDY"
     gene            6296..6372
                     /locus_tag="VI319_08380"
     tRNA            6296..6372
                     /locus_tag="VI319_08380"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6330..6332,aa:Pro,seq:cgg)
     gene            complement(6381..>8225)
                     /locus_tag="VI319_08385"
     CDS             complement(6381..>8225)
                     /locus_tag="VI319_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6863903.1"
                     /translation="LEPDYLDARSNLAAALARRAGAGGTPAAGARAQVDSGNAHKDAG
                     RLDQALACYEQSVALAPDLTAGYVDIGSTIVQLGRPRDALPWFRKALALEPDLPEALY
                     GLGMAASRAGDAAGAKAALARYLRLEPNDEPAMLALAEAHSQSFELDEARAWFERALA
                     ANPGRAGAHIGLGNVLRNQGRLADAIGEYRLALERDPGAVVAYQNVLFCMMCTSAYSA
                     RDVYQEHLEFARRFERPLFAPRPSHPNPPDPRRRLRIGYVSPDLRRNIVGYYVEPILR
                     HHDRDAFEIFCYHTGARDAFSEELAGLVHGWRDVGSLSDDAIAARVREDSIDILVDLC
                     GHGAGNRILVFARRPAPVQASYLDYSTTTGLASIDWRLTTGYCDPQGADAYYSEKLYR
                     LPGTYWTYNPPLRPPIPAPPSQSAGHVTFGSFNLYYRITPEVVALWARVLLAVPGSRL
                     AIVGVADGSTQAALLAAFAASGVDAGRLSAHSVVPFARYNELLAAVDIALAPFPYNGA
                     TTMMDCLWNGVPAVALKGGETFYSRIGCSILEEAGLGRLIAEDADDYVRIAADLAADT
                     RARETLRASLRDTMERSRLRDFAGFTRGLEAAYREMWTAWCASRREAS"
BASE COUNT         1249 a         2886 c         2844 g         1246 t
ORIGIN      
        1 cgagatctgc cacggctgcc gccgctgcgt gaacctgtgc acgaccttcc cgacgctgtt
       61 cgacctcgtc gacgagagcg cgacgatgga ggtcgacggc gtcgagaagc cgaagtactg
      121 gaacgtggtc gaccagtgct acctgtgcga cctgtgctac atgaccaagt gcccgtacgt
      181 gccgccgcat ccgtggaacg tggacttccc gcacgtgatg ctgcgcgcca aggcgctgaa
      241 gttcgccaag ggcggcacgc gctttcgcga ccggctgctg tcgtcgaccg acgcgatggg
      301 gaagctcgcg gcgatcccgg tcgtcgcgca ggcggtgaat gccgccaatg cgagcccgcc
      361 cgtgcgcgcg gtcatggaga aggcgctcgg cgtgcaccgg gaccgggagc tgccgccgtt
      421 cgcgccgcgg accttccgcg gcaaggccaa ggcgagcgcg tcgcgggagc cgagggcggg
      481 cgagcgcacg ccggggaagg ccgcgatctt cgcgacctgc tacgtgaact acaacgagcc
      541 cggtatcggc cacgacctgc tcgccgtgct cgaccacaac gacattccct acgtcatcgt
      601 ggacaaggaa gcctgctgcg ggatgcccaa gctcgagatc ggcgatctcg agagcgtcgc
      661 acggctgaag gaaatcaacg tgccgcgatt ggcgcgcctc gcgcgcgagg gctacgccat
      721 cctcaccccc gtgccctcgt gcacgctcat gttcaagacc gagctgcggc tgctcttccc
      781 ggaggacgcc gagacgaagc aggtggccga cgccatgttc gatcctttcg agtacttcgt
      841 cctgcggcac aaggacggcc tgctcaggac cgacttcagg aagccgctcg gccggctgtc
      901 gtaccatatc ccttgtcatt cgcgggtcca gaacatgggc cagaagacgc gcgaggccct
      961 cgcgctcgtc cccggaaccg aggtgcgcgt cgtcgagcgc tgctccgggc acgacggcac
     1021 ctggggcgtg aagagcgagc acttcgccaa ctcgatgaag atcggccgcc cggtgttccg
     1081 cctcatggcc gagggggacc cggactggat cagctccgac tgcccgatcg ccgcccgcca
     1141 gatcgagcag gggatggccg agggcgggaa ggacgtgcgc gcgaagaaag cgcatccgct
     1201 cacgctgctg cgcatggcgt acgggctcta gcatgccgcg gatcgagcgc gccagcctgc
     1261 tcacgctcga ggcgtacgcg cgcgagcgcg gcgccttccg cgcgcgcgtg atcgagcaca
     1321 agcgccaccg caccgtcgcg ttgggcgagc acgtccggct gatcttcgag gacgagctga
     1381 cgatccgcta ccaggtgcag gagatgctgc gcgtcgagcg catcttcgag gagagcggga
     1441 tccaggagga gctcgacacc tacaatccgc tcgtccccga cggccgcaac ttcaaggcga
     1501 cgctgatgct cgagtatccc gatcccgagg agcgccagcg cgagctcgcg cgcctcaagg
     1561 gcgtcgagcg caggatctgg ctcgaggtcg agggctcgcc ccgcatctgg gcgatcgcgg
     1621 acgaggatct cgagcgcgag aacgaggaga agacctcgtc ggtgcatttc ctgcgcttcg
     1681 agctcgacga gcgcgcggcg caggccgcca agcgcggcgc cgcgctctcg ctcggagtcg
     1741 atcacccgca gtgccgcgcc gcggtggcgc tgcctggtgc tgttcgcgac gccgtcgtgc
     1801 aggacctcgc atgagactgc cctccctagc ggcggcctcg ctgctcgcgg ccctgtgcgc
     1861 cggagccttc gcccaggtgc tcgcgccgca atacccgggg ggatacaagc cgcagttcga
     1921 cgaggacaag ccctgggaag agcagaagtg ggcgctcccg gcataccccg cggacgagaa
     1981 cctgctgcgc ttctacgtga gccccacgac tcccttcgaa ttcttcatcg actcgaagtc
     2041 ggtgagcgtc ggaaaggacg gcgtcgttcg ctacacgctc gtcgcgcgca gcccgagcgg
     2101 cacgtcgaac gtcagctacg agggcatccg ttgcgagtcg cgcgagcgca ggctgtacgc
     2161 gttcggggga gccgatcgga cgtggctcca ggcgcgcagc gccgactggg cgcggattcc
     2221 gtcccggcgt agctttccga acaaccagca ggctgcgctc gccgacgaat tcttctgcgt
     2281 acaaggctcg cacgtcggca gcccggacgt cgcggtcaac gcgctggccc gggcgagccg
     2341 cgagctgcgc tgagccgtca gagcaggacg aggttgtcga cgtggatgag ctccggctcg
     2401 tccacgtagc cgaggatcgc ctcgatctcg ctagaggcgc gtcccgcgat ccggcgcgac
     2461 tcctgcgcgc tgtaattcac cagcccgcgc gcgatctccc gtccgtcggg atcgacgcag
     2521 gcgatcaccg ctccgcgctc gaagtcgcct tcgacggatt tgacgccgac cggcaggagg
     2581 ctcttcccac gggcgagcag cgcccgcgcg gcgcctgcgt cgagatgcag cctgccgccg
     2641 atttgcacgt ggtcggcgag ccactgcttg cgcgcagcga gcgccgtggt ctgcggctgg
     2701 atcagcgtcc cgatctgctc gcccgccgcg atgcggcgga gcacgtcgga ctcgcggccc
     2761 gacgcgatca cggtctgcgc gcccgagcgc gccgcgcgct tcgcggcgag caccttggtg
     2821 agcatgccgc cgcgcgcggt ctcggagccg gcgccgcccg ccatgtcctc gaggcgcgga
     2881 tcgccggcgt tggccgagcg cacgagcgtc gcggctgcgt cgcggcgcgg gtcgctcgta
     2941 tacaggccct cctggtcggt gaggatgacg aagatatcgg cctcgatcag gttcgtcacc
     3001 agcgcgccca gcgtgtcgtt gtcgccgaag cggatctcgt cggtcacgac ggtgtcggtc
     3061 tcgttgatga ccgggacgat gccgagcgag agcagcgtga gcagcgtcga gcgcgcgttg
     3121 agatagcgct gccggtcggc gaggtcggcg tgcgtgagga gcacctgcgc cgtgcgcagt
     3181 ccgtggccgc ggaagcacga ttcgtagacc tgcgccaggc ccatctggcc caccgcggcc
     3241 gcggcctgca gctcgtgcat ggtgcgcggc ctgcggctcc acccgaggcg ctgcatgccc
     3301 tcggcgatcg cgccgctcga gaccaggatc acctcgtggc ctgcgccgcg cagctcggcg
     3361 acctgcgccg cccagcgtcc gatcgcggcc gcgtcgaggc cctgcccgtg gttcgtgacc
     3421 agcgagctgc cgaccttgac gacgaagcgg cgagcggcct tcatcgcgtc tttcccgtct
     3481 ccagaaaatg catgaccgcg gagctgagct cggtgcagcc cgcgcccgtg agcgcggaga
     3541 cgccgaaatg cggcccgcgc cagcgcagct tccggacgat ggccgccgcc gccggctcgc
     3601 gctcttcggc gggcagcata tccattttgt tgagcaccag ccagcgcgcc ttgcggtgca
     3661 ggcccgggtc gtacttcttc agctcggccg cgatggcgcg cgcgtcgcgc gcggggtcgg
     3721 ccccgggctc gagcggcgcg acgtcgatca cgtgcagcag gaggcgcgtg cgcgcgagat
     3781 gccgcaggaa ctggtgcccc aggccggcgc cctcggcggc gccctcgatc aggcccggga
     3841 tgtcggcgat cacgaagctg cggtcgactc cgagcctcac cacgccgaga tacggctgca
     3901 gcgtggtgaa ggggtagtcc gcgaccttcg gccgtgcggc cgacaccgcg cggatgagcg
     3961 tcgacttgcc cgcgttcggc agcccgagca ggccgacgtc ggccagcacc ttgagctcga
     4021 gcttgagctt gcggttctcg ccttcgccgc cgggcgtcgc cttgcgcggc gcgcggttga
     4081 tgctcgactt gaagttgacg ttgccgaggc cgccttcgcc gccgcgcgcg agcagcgccc
     4141 gctcgccgtc cttgtcgaga tcgcagatcg cctggcccgt ttccgcgtcc gcgatgaccg
     4201 tcccgaccgg catgcgcagc accacgtcgt cggcgccgcg gccgttcttc tgcccgccct
     4261 ggccgcgctc gccgtccttc gcgcgatgaa tgcgtgcgaa gcgatagtcc accagcgtat
     4321 tgatgttgcc gtcggcgacc gcccacacgc tgccgccgcg tccgccgtcg ccgccgtcgg
     4381 ggccgccgcg cggcacgtac ttctcgcgcc ggaagctgac gacgccgttc ccgcccttgc
     4441 cggcgtgcac ctcgatcacg gcttcgtcga tgaacttcat agaaacaaaa aagccctacc
     4501 gcgtggatag ggctttcgtc ggagtgaagg gacgctcagt tcggactcat gccgggacca
     4561 cgctcaccgt ctgcctgcgg ctcgggcccc tcacggcgaa cttcaccttg ccgtcgacct
     4621 tcgcgaacag cgtatggtcc ttgcccatgc cgacgttctc acccgcatgg atgcgcgtgc
     4681 cgcgctggcg cacgaggatg ctccccgcga ggacgatctg gtcgccgtag cgcttcacgc
     4741 ccagccgttt cgactcggag tcgcgcccgt tgcgcgagct gccgcctgcc ttcttgtgtg
     4801 ccatgtgttt gcctcagccc gaaattccgg tgatctcgat ttccgtatag cgctggcggt
     4861 gcccggtcgt gcgcttggag ctcttgcgcc ggcgcagctt gaagatcttc agcttctcgc
     4921 cgagcacgtg cgacacgacc ttcgccttca ccttggcgcc cggaacgacc ggcgtgccga
     4981 tcttgagcga ctcgccctcg ccgacggcga gcacttggtc gagcaccacc tcggcgccga
     5041 cctcggccgc gagcttctcg accctcagct tctcgccggc ggacacgcgg tactgcttgc
     5101 cgccggtctt cacgaccgca tacatgagac ttctcccgag aggggccgcc gcctttcgat
     5161 cagggcttgc tgcggcaccg gaaaaccgca aattatacat gccgttagac tggccggtca
     5221 aagggcatgt aatattctcg cccagcccgc cgaaacgccc gccgcgagac cccgagagtg
     5281 cccctcgaat cgatccagaa gctgatcgcc gccgacatgc aggcggtcga cgcgctgatc
     5341 cgcgcgcggc tcgattccga cgtggtcctg atccggacca tcgcagagta catcatcggc
     5401 ggcggcggca agcgcctgcg gcccgcgctg gtgctgctcg cctccggcgc gctcggctac
     5461 cgcggccgcg accaccacga gctcgctgcg gtaatcgagt tcatccacac cgcgacgctg
     5521 ctgcacgacg acgtggtcga cgagtcggag ctgcgccgcg gccgcaagac cgcgaacgcc
     5581 gagttcggta acgcggcgag cgtgctcgtc ggcgatttcc tgtactcgcg tgcgttccag
     5641 atgatggtgg ccttgagcaa catgcgcgtg atggaggtgc tagccgacgc gaccaacacc
     5701 atcgccgagg gcgaggtgct gcagctgctg aacagccaca acacgcgcgt ctcggagcag
     5761 cagtacctcg aggtcatccg gcgcaagacc gcgaagctgt tcgaggccgc cgcgcagctc
     5821 ggcggcatcc tcggcggcgc cggctccgcg gcggaatccg gtctcgcgcg ctacggcatg
     5881 catctcggga cggcgttcca actgatcgac gacgtgctcg actactcggg cgatgccgcg
     5941 cacacgggca agaacctcgg cgacgacctc aacgaaggca agccgacgct gccgctgatc
     6001 tacgtcatgg agcgcggcgc agaggcgcag gcgcgcacgg tgcgccaggc gatcgagcac
     6061 ggaggccggg gcgagttcgg agacgtcctg gcggcgatcc agtcgacggg cgcgctcgac
     6121 tatgcgcggg ccaaggcgac cgaggaggcg aagaccgcgc gcgacgcgct cgcgccgctg
     6181 gccgattcaa agcacaagga atgtctgcta gaattggcga ccttcgcggt cgagcgcgat
     6241 tactgaagtc atcgatcagg ttcccggcgc tcttcctcat gatagcaacg cggttcgggg
     6301 tgtagctcag cctggtagag tactgcgttc gggacgcagg agtcgctggt tcgaatccag
     6361 tcaccccgac cagatccgct tcacgacgcc tcccgccgcg acgcgcacca cgccgtccac
     6421 atctcccggt acgccgcctc cagcccgcgg gtgaagcccg cgaagtcgcg caggcgcgag
     6481 cgctccatcg tgtcgcgcag cgatgcgcgc agggtctcgc gcgcgcgggt gtcggcggcg
     6541 agatccgccg cgatgcggac gtagtcgtcc gcgtcctccg cgatcagccg cccgagcccg
     6601 gcctcctcga ggatgctgca gccgatgcgc gagtagaacg tttcgccgcc tttcagcgcg
     6661 accgccggca cgccgttcca caggcaatcc atcatcgtcg ttgcgccgtt gtagggaaac
     6721 ggcgcgagcg cgatgtcgac cgcggcgagc agctcgttgt acctggcgaa cgggaccacg
     6781 ctgtgcgcgg acaagcgccc ggcgtccacg ccggacgcgg cgaaggcggc gagcagcgcc
     6841 gcctgggtcg aaccgtccgc gacgccgacg atcgcgagcc gcgagcccgg cacggcgagc
     6901 agcacgcgcg cccacagcgc gaccacctcg ggcgtgatcc ggtaatagag gttgaagctg
     6961 ccgaaggtga cgtggccggc cgactgcgac ggcggcgccg ggatgggcgg ccgcaggggc
     7021 gggttgtaag tccagtacgt gccggggagc cggtagagct tctccgaata gtacgcgtcg
     7081 gctccctggg gatcgcagta cccggtcgtc agtcgccagt cgatcgacgc gagcccggtc
     7141 gtcgtcgagt aatcgagata gctcgcctgg accggcgcgg gcctgcgtgc gaacacgagg
     7201 atgcggttgc ccgcgccatg tccgcacagg tcgacgagga tgtcgatgct gtcctcgcgc
     7261 acgcgcgcgg cgatggcgtc gtcggacagc gagccgacgt cgcgccagcc atggaccagc
     7321 ccggcgagct cctcgctgaa cgcgtcgcgc gcgccggtgt gatagcagaa gatctcgaag
     7381 gcgtcccggt cgtgatgccg cagaatcggc tcgacgtagt agccgacgat gttccggcgc
     7441 aggtcgggcg agacgtagcc gatgcgcagg cgccgacgcg gatccggcgg attgggatgg
     7501 ctcgggcgcg gagcgaacag cggccgctcg aagcgccgcg cgaactcgag gtgctcctgg
     7561 tacacgtcgc gcgccgagta cgcgctcgtg cacatcatgc agaacagcac gttctggtag
     7621 gcgacgaccg cgcccgggtc gcgctcgagc gcgaggcggt attcgccgat ggcgtcggcg
     7681 agccgcccct ggttgcgcag aacgttgccc aacccgatgt gcgcgcccgc ccgccccggg
     7741 ttcgccgcca gcgcacgctc gaaccacgcg cgcgcctcgt cgagctcgaa ggattggctg
     7801 tgcgcttccg cgagcgcgag catcgcgggc tcgtcgttgg gctcgaggcg caggtaccgc
     7861 gcgagcgcgg ccttcgcgcc ggccgcgtct ccggcgcggg acgccgccat cccgaggcca
     7921 tacagcgctt ccggcagatc aggctcgagc gcgagcgcct tcctgaacca cgggagtgca
     7981 tcgcgcggac ggccgagctg cacgatcgta ctgccgatat cgacgtagcc cgcggtcaga
     8041 tccggcgcga gcgcgacgct ctgctcgtag cacgcgagcg cctggtcgag gcgcccggcg
     8101 tctttgtgcg cgttgccaga gtccacctgc gcacgcgccc cggctgccgg ggtgccgccg
     8161 gccccggcgc gccgggcgag cgcggcggcg agattggacc gggcgtcgag atagtcgggc
     8221 tcgag
//
LOCUS       DATDMW010000191         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_555530, whole genome shotgun sequence.
ACCESSION   DATDMW010000191 DATDMW010000000
VERSION     DATDMW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555530"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VI319_08390"
     CDS             <1..1010
                     /locus_tag="VI319_08390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6863904.1"
                     /translation="GWGARRVLRVDPFHIGKVWRDWSFARAARAAWRREGFDLVQSHE
                     RIPGAPLYRAGDGVHRRWLELRAASAGKLERLGMAINPYHRYVCAAERRMFEHPRLRA
                     VICISEMVRADVLRAFRVDPAKLHVIYNGVDLEYFHPRARAGREATRHELGCGPRDML
                     FLFVGSGFPRKGLAAAIDALAAAGEPRFRLWIVGRDRQQARFEERARAAGLGERVRFL
                     GGQDDVRPFYAAADCLVMPSRYEPFGSTALEALASGLPVIASTRSGASEVLEPGVSGW
                     VCEPDDAQGLARLMKLADAAAGSPTMREAARRGAERYGIDAMAAKLVGLYATLGAQRG
                     A"
     gene            1012..1953
                     /locus_tag="VI319_08395"
     CDS             1012..1953
                     /locus_tag="VI319_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6863905.1"
                     /translation="MHMLTVFDEKEVLSRAVAAAGCTVRRVAADELPPPREGERYFYF
                     GNVFEQIKQPLRLSRLRRRLAADGAPYVWWNRDAPWNCAIKPLRRLLVHASRPVDIHL
                     AHSLQSVSLFGEPVRYFPNAAQVDRYQLAGRSLESLRDPATYRWDVSFVGTLNPAFRQ
                     VRQRVAFFAELAPRLEREGISFHLVDTSVGSAVGLAEQVHTIQASRINLSVGAVCDDP
                     VPSWGLPERCFGVGACGGFLLCDERRHGPETFPAQAWESFSSVEECVARIKFFLGHFP
                     VARDKAEALHHDVMEHHTYEARARELLRLVAAWRGSR"
     gene            1935..>2155
                     /locus_tag="VI319_08400"
     CDS             1935..>2155
                     /locus_tag="VI319_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863906.1"
                     /translation="MAGLALIESGAGALYVRLLSYVRPYAAVFAISVLGMVLAAATEP
                     LFPALIKPLLDGGFAKGGSHPPIVFAGAIV"
BASE COUNT          260 a          777 c          776 g          342 t
ORIGIN      
        1 agggctgggg cgcgcgccgc gtactgcgcg tcgacccctt ccacattggc aaggtctggc
       61 gcgactggtc gtttgcgcgc gcggcgcgtg ccgcctggcg acgcgagggc ttcgacctcg
      121 tgcagtcgca cgagcgtatc cccggcgcgc cgctctaccg cgcgggcgac ggcgtgcacc
      181 ggcgctggct cgagctgcgc gcagcgagcg ccgggaagct ggagcgtctc ggcatggcga
      241 tcaatccgta ccaccgttac gtgtgcgcgg cggagcggcg catgttcgag catccgcggc
      301 tgcgcgccgt catctgcatc tccgagatgg tgcgcgccga cgtgctgcgc gcctttcgcg
      361 tcgacccggc caagctgcac gtgatctaca acggcgtcga cctcgagtac ttccatccgc
      421 gcgcccgcgc cggccgcgaa gccacgcggc acgagctcgg ctgcggcccg cgcgacatgc
      481 tgttcctctt cgtcggctcg ggcttcccgc gcaagggtct tgccgcagcg atcgatgcgc
      541 tcgccgcggc cggcgagccg cgttttcgtc tgtggatcgt cggcagggac cggcagcagg
      601 cgcgcttcga ggagcgggcg cgcgccgcgg gcctgggcga gcgcgtccgc ttcctcggcg
      661 ggcaggacga cgtgcgtccc ttctatgccg cggccgactg cctcgtcatg ccgagccgct
      721 acgagccgtt cggcagcacc gcgctcgaag cgctggcgag cgggctgccg gtgatcgcga
      781 gcacgcgcag cggcgcctcc gaggtcctcg agccgggcgt gagcggctgg gtgtgcgagc
      841 ccgacgatgc gcaggggctc gcgcgcctca tgaagctcgc cgacgccgcc gccggatcgc
      901 cgacgatgag ggaggcggcg cgccgcgggg ccgagcgcta cggcatcgac gcgatggcgg
      961 cgaagctggt cggcctctac gcgacgctcg gcgcccagcg gggcgcgtga gatgcacatg
     1021 ctcaccgtgt tcgacgaaaa ggaagttctc tcccgggcgg tcgcggctgc gggctgcacg
     1081 gtgcgccgcg tcgccgcgga cgagctgccg ccgccgcgcg aaggcgagcg gtatttctac
     1141 ttcggcaacg tgttcgagca gatcaagcag ccgttgcggc tctcgcgcct gcggcgccgg
     1201 cttgccgcgg acggcgcgcc ttacgtctgg tggaaccgcg atgcgccgtg gaactgcgcg
     1261 atcaagccgc tgcggcgcct gctggtccat gcttcgcggc cggtcgacat ccacctcgcc
     1321 cattcgctgc aaagcgtgtc gctcttcggc gagcccgtgc gctatttccc gaacgccgct
     1381 caggtcgacc gctaccagct cgccggccgc tcgctcgaat cgctgcgcga cccggcgacg
     1441 taccgttggg acgtcagctt cgtcggcacg ctgaaccccg cgttccggca ggtccggcag
     1501 cgcgtcgcct tcttcgccga gctcgcgccg cggctcgagc gcgagggcat ctccttccac
     1561 ctcgtcgaca cgagcgtcgg ctcggcagtc gggctcgcgg agcaggtgca caccatccag
     1621 gcgagccgga tcaacctgag cgtcggcgcg gtgtgcgacg acccggtgcc gagctggggg
     1681 ctgcccgagc gctgcttcgg cgtcggcgcg tgcggcgggt tcctgctgtg cgacgagcgc
     1741 cgccatggcc ccgagacctt ccccgcgcaa gcctgggagt cgttctcttc ggtcgaagag
     1801 tgcgtggcgc ggataaaatt tttcctcggg cactttcccg tggcccgcga caaggcggaa
     1861 gcgctgcacc acgacgtcat ggagcaccac acctacgagg cgcgcgcgcg cgagctgctc
     1921 cggctcgtcg ccgcgtggcg gggctcgcgt tgatcgagtc gggcgccggc gcgctctatg
     1981 tgcggctgct ttcctacgtc cggccgtacg cggccgtgtt cgccatctcg gtgctcggca
     2041 tggtcctcgc cgcggcgacc gagccgctct tcccggcgct catcaagccg ctcctcgacg
     2101 gcggattcgc gaagggcggc agccatccgc cgatcgtgtt cgccggcgcg atcgt
//
LOCUS       DATDMW010000192         2352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_680343, whole genome shotgun sequence.
ACCESSION   DATDMW010000192 DATDMW010000000
VERSION     DATDMW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680343"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VI319_08405"
     CDS             <1..719
                     /locus_tag="VI319_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY6863907.1"
                     /translation="GRPAIVADPQPPETVLEAARRVGADLRLIGRDFGYRVQGGQWMF
                     WGREGRRPGLAHPALRGASQLGNASAALAALETQRGRIPVGAQDVRTGLARAELPGRF
                     QVLPGRPTVILDVAHNPQAAAVLAENLGAMGFYPETYAVFGMLKDKDIAGVCAALKGR
                     VSAWFAASLTGPRAASSADLARAIRESGAGGEVTQYASPAQAYAAARSRAGENDRILV
                     FGSFHTVAEIMAGQSGTRGT"
     gene            734..1342
                     /locus_tag="VI319_08410"
     CDS             734..1342
                     /locus_tag="VI319_08410"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY6863908.1"
                     /translation="MAETQDALMLKRRARRRLVGAIALVLLVVIVLPVILDQEPKPVR
                     HDLTVQIPSQDAGRFGARVLQAPAPAPAKPKPEAAAAPAEEKPEAKAPEAHKTAPKST
                     KPADKRAASRKDDEAKRALALLNDTGYVVPIGVFANPENAQKARDQAAAAGFAAYTEK
                     IQSKEGEQTRVRAGPFGTRESAERARDRLKALGVPVGQVTSK"
     gene            1339..1827
                     /locus_tag="VI319_08415"
     CDS             1339..1827
                     /locus_tag="VI319_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY6863909.1"
                     /translation="MTWFDYGVLIVLVLSVVVSMLRGLALEIVSLGGWIAAFVLAVGF
                     GGALGEYMPESLGPGLRPVAGFFALFLGALVVAGFIGLLVQRMVRAAEWTWTDRALGG
                     AFGMLRGLIIVLGLVMIGGMTALPRDPFWRNAVLSGPLETMVLGLRPLLPDRIAQRIH
                     YR"
     gene            1839..>2352
                     /locus_tag="VI319_08420"
     CDS             1839..>2352
                     /locus_tag="VI319_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY6863910.1"
                     /translation="MCGIIGVVAKSPVNQLLYDGLLLLQHRGQDAAGIVTSEHKQFHM
                     HKGPGLVRDVFRTRNMRNLGGEMGVAHCRYPTAGSAFNTAEAQPFYVNSPFGIVLGHN
                     GNLTNSEALKQEMFRQDLRHINTNSDSEVLLNVLAHELEKASVKLRLDPQTIFTAVAA
                     VHKRVRGAYAV"
BASE COUNT          354 a          807 c          858 g          333 t
ORIGIN      
        1 cggggcggcc cgcgatcgtc gccgatcccc agccgccgga gaccgtgctc gaggcggcgc
       61 gccgcgtcgg cgcggacctg cggctgatcg gccgcgactt cggctaccgc gtgcagggcg
      121 ggcagtggat gttctgggga cgcgaggggc gccgtccggg ccttgcacat cccgcgctgc
      181 gcggcgcgag ccagctcggc aacgcgagcg ccgcgctcgc cgcgctcgag acgcagcgcg
      241 gccgcatccc ggtcggcgcg caggacgtgc gcaccgggct cgcgcgcgcg gagctgccgg
      301 ggcgcttcca ggtgctgccg ggacgtccga cagtgatcct cgacgtcgcg cacaacccgc
      361 aggcggccgc ggtgctcgcg gagaacctcg gcgcgatggg cttctatccg gaaacctacg
      421 cggtgttcgg gatgctcaag gacaaggaca tcgccggcgt gtgcgcggcg ctcaagggac
      481 gcgtctccgc gtggttcgcg gcgagcctca ccggacctcg cgccgcctcg agcgccgatc
      541 tcgcgcgcgc gatccgcgaa tcgggcgcgg gcggcgaggt gacgcagtac gcgtcgccgg
      601 cgcaagcgta tgccgcggcg cggagtcggg cgggtgaaaa tgatagaatt ctggttttcg
      661 gatccttcca caccgtggcc gaaatcatgg ccgggcagtc cggaacgcgc gggacctgac
      721 tcggagcgtc gccatggcag aaacccagga tgcattgatg ctgaagcgcc gggcgcgccg
      781 gcggctcgtg ggcgcgatcg cgctggtgct gctcgtcgtg atcgtgcttc ccgtgatcct
      841 ggaccaggag cccaagccgg ttcgccacga cctgacggtg cagatccctt cgcaggacgc
      901 gggcaggttc ggcgcgcgcg tgctgcaggc tcccgcgccg gcgcccgcga aaccgaaacc
      961 cgaggccgcc gccgcgcccg ccgaggagaa gcccgaagcg aaggcgcccg aagcgcacaa
     1021 gaccgcgccg aaatcgacga agcccgccga caagcgggcg gcctcgcgca aggacgacga
     1081 ggcgaagcgc gcgctggccc tgctgaacga caccggctac gtggtcccga tcggcgtctt
     1141 cgccaacccg gaaaacgccc agaaggcgcg cgaccaggcg gcggccgccg gattcgccgc
     1201 ctacacggag aagatccaga gcaaggaagg cgagcagacg cgcgtgcgcg ccggcccgtt
     1261 cggcacgcgc gagtccgcgg agcgcgcgcg cgacaggctg aaagcgctcg gcgttcccgt
     1321 cggccaggtg acgtcgaagt gacctggttc gactatgggg tgctcatcgt gctcgtgctc
     1381 tcggtggtcg tcagcatgct gcgcggcctc gcgctcgaga tcgtctcgct cggcggctgg
     1441 atcgcggcgt tcgtgctcgc ggtcggcttc ggcggagcgc tcggcgaata catgccggag
     1501 agcctcggcc cgggcctgag gcccgtcgcc ggcttcttcg cgctgttcct cggcgcgctg
     1561 gtggtcgcag ggttcatcgg gctgctggtt cagcgcatgg tgcgcgcagc cgagtggacc
     1621 tggaccgacc gtgcgctcgg cggcgcgttc ggcatgctgc gcgggctgat catcgttctc
     1681 gggctggtga tgatcggcgg catgacggcg ctgccgcgcg acccgttctg gcgcaacgcg
     1741 gtgctgtccg ggccgctcga gaccatggtg ctggggctgc ggccgctcct gcccgaccgc
     1801 atcgcgcagc gcatccatta ccggtaggga aaagagctat gtgcggaatc atcggagtcg
     1861 tggcgaagag tcccgtgaac cagctgctct acgacgggct gctgctcctg cagcaccgcg
     1921 gtcaggacgc ggccgggatc gtgacgtccg agcacaagca gttccacatg cacaaggggc
     1981 cgggcctggt gcgcgacgtg ttccgcacgc gcaacatgcg caacctcggc ggcgagatgg
     2041 gcgtggcgca ttgccgctac ccgaccgcgg gatcggcgtt caacacggcc gaggcgcagc
     2101 cgttctatgt caactcgccg ttcggcatcg tcctcggcca caacggcaac ctcacgaact
     2161 ccgaggcgct caagcaggag atgttccggc aggacctgcg gcacatcaac acgaactccg
     2221 actccgaggt cctgctcaac gtgctcgcgc acgagctgga gaaagcctcg gtcaagctgc
     2281 gcctggatcc gcagacgatc ttcaccgcgg tggcggccgt gcacaagcgc gtgcgcggcg
     2341 cctacgcggt ga
//
LOCUS       DATDMW010000193         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1178760, whole genome shotgun sequence.
ACCESSION   DATDMW010000193 DATDMW010000000
VERSION     DATDMW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178760"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VI319_08425"
     CDS             <1..191
                     /locus_tag="VI319_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6863911.1"
                     /translation="LMDALLEQGLWVPAIRPPTVPAGTARLRISLSAAHEAEEVDRLV
                     EALRGLAARRHARAALAR"
     gene            205..1518
                     /gene="bioA"
                     /locus_tag="VI319_08430"
     CDS             205..1518
                     /gene="bioA"
                     /locus_tag="VI319_08430"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY6863912.1"
                     /translation="MNERSPSERSIAAVWHPCTQMKTHERVPPVAIARGSGAWLYDLE
                     GRRYLDAISSWWVNLFGHANPRINAALRDQLERLEHVMLAGFTHEPVVELSEQLAALT
                     GGHLGHCFYASDGASAVEIALKMSAHYWRNRGLPKKHRFVALANGYHGETLGALAVTD
                     LGLFKAPYADLVIEAAKVPSPDWRLAEPGETPTDYAGRCAAALESHLQRYHTETAAVV
                     VEPLVQCAGGMAMYHPEYLRRARALCERFAVHLIADEIAVGMGRTGTMLACEQAAITP
                     DFLCLSKGLSGGTLPLSVVLASDAVYRAFYDDDPARGFLHSHSYTGNPLACRAALATL
                     AIFRDDDVIAGNRARAQKLTRMLAPLAAHPRVRAFRNTGMIWAFEVEGAGPQFPARFH
                     AAALERGLLLRPLGGTVYFMPPYVLDDEQCELLASETLALVDAVE"
     gene            1523..2197
                     /gene="bioD"
                     /locus_tag="VI319_08435"
     CDS             1523..2197
                     /gene="bioD"
                     /locus_tag="VI319_08435"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY6863913.1"
                     /translation="MPGVFVTGTDTGVGKTLVACALLRRLAADGASTVGMKPVAAGCE
                     QTPAGRTNEDVEALIAASTVRADPALVNPYALREAIAPHLAAAHESVRIDLEHLVACY
                     TRLAGIARWVVVEGVGGFRVPLNESHDAADLARMLALPVVLVVGMRLGCLNHATLTRE
                     AIASRGLGLAGWVANRIDPAMARCDENIEFLKRALDAPFLGEIPHVEHAEGRLQTAID
                     VISFPG"
     gene            2214..>3207
                     /locus_tag="VI319_08440"
     CDS             2214..>3207
                     /locus_tag="VI319_08440"
                     /inference="COORDINATES: protein motif:HMM:NF028467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HEY6863914.1"
                     /translation="MAFEERPIFLSTMPPDARERWLALAVVVASAVLFAVAAPLATMP
                     LLPVPAFIPAYQSAVFVNDAVTAVLLFGQYAILGSVAVLVLAAGYVFTACMAVIHALT
                     FPGLASSGGLLGAGPQSTAWLYMFWHAGFPVAVIAYAVLARRVRGEARAKPSRPGIAI
                     TSAAAAAMVCAGTAAAVATAGHDALPPIMQGNRYTPAMIVVVSGVWGASLLALAVLWL
                     RRRRSVLDVWLMVVMCAWLFDIALSAVLNAGRFDLGFYAGRAYGLLAASFVLIVLLLE
                     NGVLYARLADAHAGERQARRRVEEKTTELVIANKELDAFSHSVSHDLRAPLRAVD"
BASE COUNT          416 a         1171 c         1124 g          496 t
ORIGIN      
        1 agctcatgga cgcgctgctc gagcaggggc tctgggtgcc ggcgatccgg ccgccgaccg
       61 tgccggcggg cacggcgcgt ctgcgcatct cgctctcggc cgcgcacgag gccgaagaag
      121 tcgaccgcct ggtcgaggcg ctgcgcggac tcgccgcgcg gcgccacgca cgcgccgcgc
      181 tcgcgcgctg agcgcgcacg tcccgtgaac gagcgcagcc cttccgagcg cagcatcgcc
      241 gcggtctggc acccgtgcac gcagatgaag acgcacgagc gcgtcccgcc cgtggccatc
      301 gcgcgtggca gcggcgcctg gctctacgat ctcgaaggcc gccgctacct ggacgcgatc
      361 agctcctggt gggtgaacct gttcgggcac gccaacccgc gcatcaacgc ggcgcttcgc
      421 gatcagctcg agcggctcga gcacgtgatg ctggccggct tcacgcacga gcccgtcgtc
      481 gagctgtcgg agcagctcgc ggcgctgacg ggcgggcacc tcggccactg cttctacgcc
      541 tccgacggcg cgtccgcggt cgagatcgcg ctcaagatga gcgcgcatta ctggcgcaac
      601 cggggactgc cgaagaagca ccgcttcgtc gcgctggcca atggctatca cggcgagacg
      661 ctcggggcgc tggcggtcac ggacctcggg ctgttcaagg ccccttacgc cgacctcgtg
      721 atcgaggcgg caaaggtgcc gagccccgac tggcgactgg ccgaacctgg cgagaccccc
      781 accgactacg ccgggcgctg cgccgcggcg ctcgagtcgc atctgcagcg gtaccacacg
      841 gaaaccgcgg ccgtcgtcgt cgagccgctg gtccaatgcg ccggcggcat ggcgatgtac
      901 caccccgaat acctgcgccg cgcgcgcgcg ctgtgcgagc gcttcgccgt ccacctgatc
      961 gccgacgaga tcgccgtcgg catggggcgc accggcacca tgctcgcctg cgagcaggcg
     1021 gcgatcacgc ccgatttcct gtgcctgtcg aagggactga gcggcggcac cctgccgctg
     1081 tcggtggtgc tcgcgagcga cgcggtctac cgcgccttct acgacgacga ccccgcccgg
     1141 ggctttctcc actcccactc gtatacaggg aaccccctcg cctgccgcgc cgcgctcgcg
     1201 acgctcgcca tcttccgcga cgacgatgtg atcgccggca accgcgcgcg cgcgcagaag
     1261 ctgacccgga tgctggctcc cctcgccgcg cacccgcggg tgcgcgcctt ccggaacacc
     1321 ggcatgatct gggcgttcga ggtcgaaggc gccggtccgc aattcccggc gcgcttccac
     1381 gccgccgcgc tcgagcgcgg gctgctgctg cggccgctcg gcggcacggt ctatttcatg
     1441 ccgccgtacg tcctcgacga cgagcagtgc gagctgctcg cctcggagac gctcgcgctg
     1501 gtcgacgcgg tggaatagcg cgatgccggg cgtctttgtc accggcaccg acacgggcgt
     1561 cggcaagacg ctggtcgcct gcgcgctgct gcgccggctc gccgcggacg gcgcgtcgac
     1621 cgtcgggatg aagccggtcg cggcgggctg cgagcaaacg ccggccggga ggaccaacga
     1681 ggacgtcgaa gcgctgatcg ccgcgagcac ggtgcgggcg gacccggcgc tcgtgaatcc
     1741 atatgccctg cgcgaggcga tcgcgccgca cctcgccgcg gcgcacgagt cggtccgcat
     1801 cgacctcgag cacctcgtcg cctgctacac ccggctcgcc gggatcgcgc gctgggtcgt
     1861 cgtcgaaggc gtcggcggct tccgcgtgcc gctgaacgaa tcgcacgacg cggccgacct
     1921 cgcgcggatg ctcgcgctgc cggtggtcct cgtcgtcggc atgcgcctcg gctgcctcaa
     1981 ccatgcgacg ttgactcggg aggcgatcgc gtcgcgcggg ctggggctcg ccggctgggt
     2041 cgccaaccgc atcgaccctg cgatggcgcg ctgcgacgag aacatcgaat tcctgaaacg
     2101 cgcgctcgac gcgccgttcc tcggagaaat cccgcacgtc gagcacgccg aagggcgcct
     2161 ccaaacggcc atcgatgtga tatctttccc cgggtaatcc aggggaaagg cgcatggcgt
     2221 tcgaagagcg cccgatattc ctgtcgacga tgccgcccga cgcgagggag cgttggctgg
     2281 cgctggcggt cgtcgtggcg tccgccgtgc tgtttgccgt cgccgcgccc ctggccacga
     2341 tgccgctgct ccccgtcccg gcgttcatcc ccgcgtacca gtcggccgtc ttcgtcaacg
     2401 atgccgtgac cgcggtgctg ctgttcgggc agtacgcgat actgggctcg gtcgcggtgc
     2461 tggtgctggc ggcgggctat gtcttcacgg cttgcatggc ggtgatccac gccctgacgt
     2521 ttccggggtt ggcctcctcc ggcggactgc tcggcgcggg gccgcagagc accgcatggc
     2581 tctacatgtt ctggcacgcg ggcttcccgg tcgcagtgat cgcgtacgcc gtcctcgccc
     2641 gccgcgtgcg cggcgaggcg cgcgccaagc cgtcccgtcc gggcatcgcg atcacgagtg
     2701 cggccgcggc ggcaatggtg tgcgccggca cggcggccgc cgtcgccacc gcagggcacg
     2761 atgcgctgcc cccgatcatg cagggcaacc gctacacgcc cgcgatgatc gtcgtcgtct
     2821 cgggcgtgtg gggcgcgagc ctgctcgccc tcgccgtgct gtggctgcgc cgccggcgct
     2881 cggtcctcga cgtgtggctg atggtcgtca tgtgcgcatg gctgttcgat atcgcgcttt
     2941 ccgcggtgtt gaacgccggg cgcttcgatc tcggctttta cgcgggccgg gcctacggcc
     3001 tgctcgccgc gagcttcgtc ctgatcgtgc tgctgctcga gaacggcgtg ctctacgcgc
     3061 ggctcgcgga cgcgcacgcg ggcgagcgcc aggcgcgccg gcgcgtcgag gagaagacca
     3121 cggagctggt gatcgccaac aaggagctgg atgcgttttc gcattcggta tcgcacgacc
     3181 tgcgcgcgcc cctgcgcgcc gtcgacg
//
LOCUS       DATDMW010000194         8904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2176344, whole genome shotgun sequence.
ACCESSION   DATDMW010000194 DATDMW010000000
VERSION     DATDMW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176344"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VI319_08445"
     CDS             complement(<1..864)
                     /locus_tag="VI319_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863915.1"
                     /translation="MLASYLFAWLAVLGISLGAMANLMVHTLTHGRWGLPLRPPLLAA
                     VRLLPLVALLFLPVLIGYRHIFPWAGDEAAAEHAAWWLNAPFFLARSVAYLAIWNWLA
                     WLWLRADARSGAPWAPTDAARRASAAGLVVYTFTISLAATDWIASLTPAWYSSGFGLV
                     VGVGQMLAGAAFGVAAFCIAAPENEETRALAHDHGNLLLMYVMTWAYLAFTQFLIIWA
                     ENLPHEIAWYVPRLQTGWWGLGAFLVAFQFFLPFLILLSRGAKRAPRFLAGLATALLV
                     AYVANVFWLVLP"
     gene            complement(864..1463)
                     /locus_tag="VI319_08450"
     CDS             complement(864..1463)
                     /locus_tag="VI319_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6863916.1"
                     /translation="MTRLACALAVVLALAGCERIGRNMYDQPRDKPLRPSPLFPDGSS
                     SRPGVPGTVARSEGVLSGTSSGRAGADEALRRAAAERAQSLPKPVTTAMLRRGRERFD
                     IYCAPCHSPVGDGDGMVARRGFPHPPSYHVERLRAAPDRHFYDVITNGHGVMASYANR
                     VEPEDRWAIVAYIRALQRSQWAAESDVPPDELARLKEQR"
     gene            complement(1460..1993)
                     /locus_tag="VI319_08455"
     CDS             complement(1460..1993)
                     /locus_tag="VI319_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEY6863917.1"
                     /translation="MSAPLWGLMAELADADALLAAAQRAGAAGWRRAEAYSPFPVEGV
                     AEALGFARNRVPLLALLGGVAGGVGGYFMQWYSAVVSYPLNIGGRPLHSWPSFIPATF
                     ELTVLGAALAAVFGMLALNGLPRLRHPVFDVPEFDTATRNRFFLCLRADDPKFDDDAA
                     RRFLEALRPLAILEVPA"
     gene            complement(2004..3335)
                     /gene="nrfD"
                     /locus_tag="VI319_08460"
     CDS             complement(2004..3335)
                     /gene="nrfD"
                     /locus_tag="VI319_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY6863918.1"
                     /translation="MAEVLRGRWGYASITDKIADIVLTRPVHWVWALVFGAAASGALF
                     FFGAMGYLFVRGIGIWGVDIPVAWGYAIGNFVWWIGIGHAGTFISAFLLLLRQKWRNS
                     INRFAEAMTLFAAGIAGVWPILHLGRPWFFYWLTPYPNVMELWPQWRSPLVWDIFAIS
                     TYLIVSLLFWYVGLVPDLATLRDRAHTRAKQVVYGLLALGWRGEARHWARFETAYLLL
                     AGLAAPLVISVHSVVSLDFAVGNTPGWHSTLFPPYFVAGALFSGFAMVLTLAIPMRHF
                     FGLQDFITARHLDHAAKIMLATGLIVAYGYFMEIFTAFYSGDEYEIYMTLNRMAGPYA
                     PVYWLLMACNVIAPQLLWFRRVRRTVWLLFVLSIVINVGMWSERFMIVVTSLHRDFLP
                     SSWGMFYPTGWDWSMLAGSIGLFTMLFLLFVRFLPAISIFEMREIAREERE"
     gene            complement(3337..6174)
                     /locus_tag="VI319_08465"
     CDS             complement(3337..6174)
                     /locus_tag="VI319_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY6863919.1"
                     /translation="MPPVSRALWRSVDEPAAGSDGHGPHDAHPLEFSPSRRDFMRLAA
                     ASLALAGAGCSPPAEEITPYVKSDGEPVPGVPRYFATAMSLGGAAIGVLARSDMGRPT
                     KIEGNPTHPSSLGATDVWAQAAVLELWDPRRSQTVRRGALPMSREAFFSALGARLERL
                     RARGGEGLRVLTRYVDSPTLEAQLRAVLERFPAARRHVWEPLHRDNSVEGARLAFGRV
                     LEPVYRFDRADVVVALDADFLREGPGRVRYARDFAARRRADGRAGAMSRVYAIESRPT
                     LAGAMADHRVALPVAGIERWLAQLDGQLRGAESDDPRVRAAAADLAASAGRALIVAGE
                     SLPPGAHALVHALNDALGAPGQTLQYIAPVAAAESCTASLRELTGDMHAGRVDTLLVL
                     GGNPVYDAPADLAFGDALARVPFSAHLALRRDETSAAATWHVPATHFLEHWSDARAHD
                     GTASVVQPLIAPLYGGTSPHALLAAVAGEPDADGHRRVRRTWTLRQAQGEREFENFWR
                     DSLREGVVRGSAAAPVSARPRGVPYSPPAGPAGWSVRFVEDASVRDGEFAANAWLQEL
                     PRPDSKLTWDNAALLAPGSAAVLGVRTGDLVEIEAGAARVRAPVWVLPGQAEGAIAIA
                     LGYGRGTGVDAYPLRASGSPWEVRDAHVRRSDGRHAFATTQEHARMEGRDPVRRAAIA
                     DFEAGDFDLGKEQPEESLYPEWRYEGYRWGMAIDLGACIGCNACTIACQAENNIPTVG
                     KDQVARGREMHWIRVDRYYEGDAAAPRTLFQPVPCMQCENAPCEEVCPVGATVHDSEG
                     VNVQVYNRCVGTRFCSNNCPYKVRRFNFLQFADRESESLKAMRNPEVTVRMRGVMEKC
                     NYCLQRITRARIETEREGRRIADGEVVTACQAACPAEAIVFGDLNDPKSRVIAAKRSP
                     LEYALLGELNTRPRTTYLARLFNPNPDIEEG"
     gene            complement(6162..6809)
                     /locus_tag="VI319_08470"
     CDS             complement(6162..6809)
                     /locus_tag="VI319_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY6863920.1"
                     /translation="MAQIFRPGQILGLKLALLFVLGIVAIGVVTVYKAAGGPVAPGDA
                     AQQPIPFSHKHHVGDDGIDCRYCHTSVEKSSFAGLPATETCMTCHSQLFTDAPMLAPL
                     RDSAASGKPLRWTRVHDLPDFVYFDHSIHIAKGVACVECHGRVDQMPLTRRGASLEMQ
                     WCLGCHRHPEEHLRPREDVFSMQPARVTAASSPELLRLLALVPERRREDCSTCHR"
     gene            6887..7429
                     /locus_tag="VI319_08475"
     CDS             6887..7429
                     /locus_tag="VI319_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY6863921.1"
                     /translation="MTTVLTLGHSTRPIEELVSLLRAHGVTRLVDVRTVPRSRRNPQF
                     NADALPGTLAPSGISYTHLGGLGGLRRPRQDSPNTGWRNESFRGYADYMQTGEFLDSL
                     EHLIEMARHDHVAIMCAEAVPWRCHRSLIADALLVRGVRVDEIVGADRMRAHALTPFA
                     RVDGHAITYPPEGAEGPTRA"
     gene            complement(7416..8291)
                     /locus_tag="VI319_08480"
     CDS             complement(7416..8291)
                     /locus_tag="VI319_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6863922.1"
                     /translation="MISTASPGSSGDVALRMVAAKMAAAMGHPVIVDVRPAARGAQMA
                     QALARAAPDGHTITFGTSGTYVNARFLFKNVSFDILRDFVPISLTVRSPTYVAISSTT
                     PIHTLAELIDYAKKHPGKIEYASTGAGSVFHLAGESLKLYAGIDMLHVPYAQANFGQM
                     INDWVSGRVPLYFPAYGFLAPNLGKVRPLAVLATKRTERMPDVPAVVETLPQFQTFDV
                     WWAFFAPAGTPSEVCAKIADESRAALKFPDVLARLDQLALTPVGSTPAELDAELKRDL
                     ATVGNLVQKIGLKPE"
     gene            8406..>8904
                     /locus_tag="VI319_08485"
     CDS             8406..>8904
                     /locus_tag="VI319_08485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6863923.1"
                     /translation="MRAINTHFHWYPRSVFEALAKRGDFPRAVATARGGYMVSPHEGA
                     ARTDTWADWFDLDAMLGTMDAAGAKIGCDFGVVCTTGPFGAFFSETDAAHGLAFAQAW
                     NDEMAGAVARNPGRVWASGVVPLQDPKKAVDELERAMRLGLVGVNVPSSIGPRGVTRI
                     DDPSLE"
BASE COUNT         1359 a         3122 c         3274 g         1149 t
ORIGIN      
        1 gggcagtacc agccagaaca cgttcgcgac gtacgcgacg agaagcgccg tggcaaggcc
       61 ggccaggaag cgcggcgcgc gcttcgcgcc gcgcgagagc aggatcagga acggcaggaa
      121 gaactggaag gcgacgagaa aggcgccgag cccccaccag ccggtctgca gccgcggcac
      181 gtaccaggcg atctcgtgcg gcaggttctc ggcccagatg atcaggaact gcgtgaacgc
      241 gaggtacgcc caggtcatca cgtacatgag gagcaggttg ccgtggtcgt gggcgagcgc
      301 gcgcgtctcc tcgttctccg gcgcggcgat gcagaaggcg gcgacgccga acgcggcgcc
      361 ggcgagcatc tgccccacgc ccaccaccag gccgaacccg ctcgagtacc acgccggcgt
      421 gagcgacgcg atccagtcgg tggcggcgag cgagatcgtg aaggtgtaga cgacgagccc
      481 tgccgcgctc gcgcgccgcg cggcgtcggt cggcgcccac ggcgcgcccg agcgcgcgtc
      541 ggcgcgcagc cacagccacg cgagccagtt ccagatcgcg aggtaggcga ccgagcgcgc
      601 gaggaagaag ggcgcgttca gccaccaggc cgcgtgctcg gcggcggcct cgtcgccggc
      661 ccaggggaag atgtggcgat agccgatcag caccgggaga aacagcagcg cgacgagcgg
      721 cagcagccgc accgcggcca ggagcggcgg ccgcagcggc aggccccagc gcccatgcgt
      781 gagcgtgtgc accatcaggt tcgccatcgc gccgagcgag atcccgagca cggcgagcca
      841 cgcgaagaga tacgaggcga gcatcagcgc tgctctttca ggcgggccag ctcgtcgggt
      901 ggcacgtcgc tctccgccgc ccactggctg cgctgcagcg cgcggatgta cgcgacgatc
      961 gcccagcggt cctcgggctc gacgcggttc gcgtacgagg ccatgacgcc gtgcccgttc
     1021 gtgatcacgt cgtagaagtg gcggtcgggc gccgcgcgca ggcgctcgac gtggtacgag
     1081 ggcgggtgcg ggaagccgcg gcgcgcgacc atgccgtcgc cgtcgccgac cgggctgtgg
     1141 cagggcgcgc agtagatgtc gaagcgctcg cggccgcggc gcagcatcgc cgtcgtcacc
     1201 ggtttcggca gcgactgcgc gcgctcggcc gcggcgcggc gcagcgcctc gtcggcgccg
     1261 gcgcggccgc tcgaagtgcc cgagagcacg ccctcggagc gcgccacggt gcccggcacg
     1321 ccgggccgcg acgaggagcc gtcgggaaag agcgggctcg gccggagcgg cttgtcgcgc
     1381 ggctggtcgt acatgttgcg accgatgcgc tcgcagcccg cgagcgccag cacgacggcg
     1441 agcgcgcagg cgagccgcgt cacgccggca cctccaggat cgcgagcggc cgcagcgcct
     1501 cgaggaagcg gcgcgcggcg tcgtcgtcga acttcggatc gtcggcccgc aggcacagga
     1561 agaagcggtt gcgcgtcgcc gtgtcgaatt cgggcacgtc gaacaccggg tggcgcagtc
     1621 gcggcaggcc gttcaacgcg agcatgccga agaccgcggc gagcgccgcg ccgagcaccg
     1681 tcagctcgaa ggtcgccggg atgaacgagg gccagctgtg cagcggccgc ccgccgatgt
     1741 tcagcgggta cgagacgacc gccgagtacc actgcatgaa gtagccgccg acgccgcccg
     1801 cgacgccgcc gagcagcgcg agcagcggca cgcggttcct cgcgaagccg agcgcctcgg
     1861 cgacgccctc gaccgggaac ggcgagtacg cctcggcgcg gcgccatccg gccgcgcccg
     1921 cgcgctgcgc ggcggcgagc agcgcgtcgg cgtcggcgag ctcggccatc aggccccaga
     1981 gcggcgcgct cattccggct tcctcattcg cgctcctcgc gcgcgatctc gcgcatctcg
     2041 aagatcgaga tcgcgggcag gaaacgcacg aacagcagga acagcatcgt gaacaggccg
     2101 atcgaccccg cgagcatcga ccagtcccag ccggtcggat agaacatgcc ccacgacgac
     2161 ggcaggaaat cgcggtgcag gctggtgacg acgatcatga agcgctcgct ccacatgccg
     2221 acgttgatca cgatgctgag cacgaacagc agccacaccg tgcgccgcac gcgccggaac
     2281 cacagcagct gcggcgcgat cacgttgcag gccatgagca gccagtacac gggcgcgtac
     2341 gggccggcca ttcggttcag ggtcatgtag atctcgtact cgtcgcccga gtagaaagcg
     2401 gtgaagatct ccatgaagta gccgtaggcg acgatcaggc cagtcgcgag catgatcttc
     2461 gcggcgtggt cgaggtggcg cgcggtgatg aagtcctgca gcccgaagaa gtgccgcatc
     2521 gggatcgcga gcgtgagcac catcgcgaac cccgagaaca gcgccccggc gacgaaatac
     2581 ggcgggaaca gggtcgagtg ccagccgggc gtgttgccga cggcgaagtc gagcgacacg
     2641 acgctgtgca ccgagatcac cagcggcgcc gcgaggcccg cgagcagcag gtacgcggtc
     2701 tcgaagcgcg cccagtggcg cgcctcgccg cgccagccga gcgcgagcag gccgtagacg
     2761 acctgcttcg cgcgcgtgtg ggcccggtcg cgcagcgtcg cgaggtcggg cacgagcccc
     2821 acgtaccaga agagcagcga cacgatcagg taggtgctga tcgcgaagat gtcccagacc
     2881 agcgggctgc gccactgcgg ccagagctcc atcacgttcg ggtacggcgt cagccagtag
     2941 aagaaccacg gccgcccgag gtgcaggatc ggccacacgc cggcgatgcc cgccgcgaac
     3001 agcgtcatcg cctcggcgaa gcggttgatc gagttgcgcc acttctggcg cagcagcagc
     3061 aggaacgccg agatgaaggt ccccgcgtgg ccgatgccga tccaccagac gaagttgccg
     3121 atcgcgtagc cccacgcgac cgggatgtcc acgccccaga tgccgatccc gcgcacgaac
     3181 agatacccca tcgcgccgaa gaagaagagc gctcccgacg ccgcggcccc gaacacgagc
     3241 gcccacaccc agtgcaccgg gcgcgtgagc acgatgtcgg cgatcttgtc ggtgatgctg
     3301 gcgtagcccc agcggccgcg caggacttcg gccacgtcaa ccctcctcga tgtcgggatt
     3361 ggggttgaag agccgcgcga ggtaggtcgt gcgcgggcgc gtgttcagct cgccgagcag
     3421 ggcgtactcg agcggcgagc gcttcgcggc gatgaccctg ctcttcggat cgttcaggtc
     3481 gccgaagacg atcgcctcgg cggggcaggc ggcctggcac gccgtcacca cctcgccgtc
     3541 ggcgatgcgg cggccttcgc gctcggtctc gatgcgcgcg cgcgtgatgc gctgcagaca
     3601 gtagttgcat ttttccatca cgccgcgcat gcgcacggtg acctcggggt tgcgcatcgc
     3661 tttcagggat tccgattcgc ggtcggcgaa ctgcaggaag ttgaagcgcc gcaccttgta
     3721 cgggcagttg ttcgagcaga agcgcgtgcc gacgcagcgg ttgtagacct gcacgttgac
     3781 gccttcgctg tcgtgcacgg tcgcgcccac gggacatact tcctcgcacg gcgcgttctc
     3841 gcactgcatg cacggcaccg gctggaacag cgtgcgcggc gcggcggcat cgccctcgta
     3901 gtagcggtcg acgcggatcc agtgcatctc gcgcccgcgc gcgacctggt ccttgccgac
     3961 cgtcgggatg ttgttctcgg cctggcacgc gatcgtgcac gcgttgcagc cgatgcacgc
     4021 gccgagatcg atcgccatgc cccagcggta gccttcgtag cgccattccg ggtagagcga
     4081 ctcctccggc tgctccttcc cgaggtcgaa atcgcccgcc tcgaagtcgg cgatcgcggc
     4141 gcggcggacc gggtcgcggc cctccatgcg cgcgtgttcc tgcgtcgtcg cgaaggcgtg
     4201 gcggccgtcc gagcgtcgca cgtgcgcgtc gcggacctcc cacggcgagc ccgaggcgcg
     4261 cagcgggtag gcgtcgacgc cggtgccccg tccgtagccg agcgcgatcg cgatcgcgcc
     4321 ctcggcctgg ccgggaagga cccagaccgg ggcgcgcacg cgcgcggcgc ccgcctcgat
     4381 ctcgacgagg tcgcccgtgc gcacgccgag gaccgcggcc gagccgggcg cgagcagcgc
     4441 cgcgttgtcc caggtgagct tggaatcggg gcgcggcagc tcctgaagcc acgcgttggc
     4501 ggcgaactcg ccgtcgcgca ccgaagcgtc ctcgacgaag cgcacgctcc aacccgcggg
     4561 tcccgcgggc ggcgagtagg gaacgccgcg cgggcgcgcg gagacgggcg ccgcggcgct
     4621 gccgcgcacg acgccctcgc gcagcgagtc gcgccagaaa ttctcgaact cccgttcgcc
     4681 ctgggcttgt cgaagggtcc aggtgcgccg gacccgccga tggccgtctg cgtcgggctc
     4741 ccccgccacc gcggcgagca gcgcgtgcgg cgacgtgccg ccgtagagcg gcgcgatcag
     4801 cggctgcacc acgctcgcgg tgccgtcgtg ggcgcgcgcg tcgctccagt gctcgagaaa
     4861 gtgcgtcgcc gggacgtgcc aggtcgcggc cgccgacgtc tcgtcgcggc gcagcgccag
     4921 gtgcgcggag aaaggcacgc gcgcgagcgc gtcgccgaag gcgaggtcgg cgggcgcgtc
     4981 gtacacgggg ttgccgccga gcacgagcag cgtgtcgacg cggcccgcgt gcatgtcgcc
     5041 ggtcagctcg cgcagcgacg cggtacagct ctcggccgcg gcgacgggcg cgatgtactg
     5101 cagcgtctgt ccgggcgcgc cgagcgcgtc gttcagcgcg tgcaccagcg catgcgcgcc
     5161 gggcggcagc gattcgccgg cgacgatgag cgcgcgcccg gcgctcgccg ccaggtcggc
     5221 tgccgccgcg cgcacgcgcg gatcgtcgct ctcggcgccg cgcagctggc cgtcgagctg
     5281 cgcgagccag cgctcgattc cggcgaccgg cagcgcgacg cggtggtcgg ccatcgcgcc
     5341 cgcgagcgtc ggccggctct cgatcgcgta gacgcggctc atcgctcccg cgcggccgtc
     5401 ggcgcgccgg cgcgcggcga agtcgcgcgc gtagcgcacc cgcccggggc cctcgcgcag
     5461 gaagtcggcg tcgagcgcga ccacgacgtc ggcgcggtcg aagcggtaca ccggctcgag
     5521 cacgcgaccg aacgcgaggc gcgcgccttc gacggaattg tctcggtgca gcggctccca
     5581 cacgtggcgg cgcgccgcgg ggaagcgctc gagcacggcg cgcagctgcg cttccagcgt
     5641 gggcgagtcg acgtagcgcg tcagcacgcg caagccctcg ccgccgcgcg cgcgcagccg
     5701 ctcgagcctc gcgccgagcg ccgagaagaa ggcctcgcgc gacatcggca gcgcgccgcg
     5761 gcgcacggtc tgcgagcgcc gcgggtccca cagctcgagc accgcggcct gcgcccagac
     5821 gtcggtcgcg ccgaggctcg acgggtgcgt gggattgccc tcgatcttgg tcggccgtcc
     5881 catgtcgctg cgcgcgagca cgccgatcgc ggcgccgccg aggctcatcg ccgtcgcgaa
     5941 gtagcgcggc acgccgggca ccggctcgcc gtccgatttc acgtagggcg tgatctcctc
     6001 ggcaggcggc gagcagcccg cgcccgcgag cgcgagcgag gcggcggcga gccgcatgaa
     6061 gtcgcgccgc gaaggggaga actcgagcgg gtgcgcgtcg tgcgggccgt gcccgtcgct
     6121 tccggcggcc ggctcgtcga cgctgcgcca gagcgcccgg ctcaccggtg gcatgtcgag
     6181 caatcctcgc ggcggcgctc cgggacgagc gcgagcagcc gcagcagctc ggggctgctc
     6241 gccgcggtga cgcgcgccgg ctgcatcgag aacacgtcct cgcgcgggcg caggtgctcc
     6301 tccggatggc ggtggcagcc caggcaccac tgcatctcga ggctcgcgcc ccggcgcgtg
     6361 agcggcatct ggtcgacgcg gccgtggcac tcgacgcagg cgacgccctt ggcgatgtga
     6421 atgctgtgat cgaagtagac gaaatccggc aggtcgtgca cgcgcgtcca gcgcaggggc
     6481 ttcccgctcg ccgcgctgtc gcgcagcggc gcgagcatcg gcgcatcggt gaacagctgc
     6541 gagtggcagg tcatgcaggt ctcggtcgcg ggcagcccgg cgaacgagct cttctcgacc
     6601 gacgtgtggc agtagcggca gtcgatgccg tcgtcgccga catggtgctt gtggctgaag
     6661 ggaatcggct gctgcgcggc gtcgccggga gccaccgggc ccccggccgc cttgtacact
     6721 gtcaccacgc cgatcgccac gattccgaga acgaacagga gcgcgagctt gaggcccagg
     6781 atttgaccgg gcctgaagat ctgcgccatg ggtgattgag cgcgagggga accgggagtt
     6841 cccggggctc ccggaacccg cacgcccgga gagagtcgat cagcgcatga cgaccgtcct
     6901 gaccctcggc cattcgacgc ggcccatcga ggagctcgtc agcctgctgc gagcgcacgg
     6961 cgtgacgcgg ctcgtcgacg tgcgcaccgt gccccgctcg cgccgcaatc cgcagttcaa
     7021 cgccgacgcg ctgcccggga cgctcgcgcc ctcgggtatc tcgtatacac acttaggcgg
     7081 actcggggga ttgcggcgtc cgcgccagga ttccccgaac accggctggc gcaacgagtc
     7141 gttccgcggc tacgcggact acatgcagac gggggaattc ctggacagcc tcgagcatct
     7201 gatcgagatg gcccggcacg accacgtcgc gatcatgtgc gccgaagccg ttccgtggcg
     7261 ctgccaccgc tcgctgatcg ccgatgcgct gctggtgcgc ggcgtgcgcg tggacgagat
     7321 cgtgggcgcg gaccgcatgc gcgcccacgc gctgacgcct ttcgcgcgcg tggacggcca
     7381 cgcgatcacg tatccgcccg aaggcgccga agggcctact cgggcttgag cccgatcttc
     7441 tgcacgagat tgccgaccgt cgcgaggtcg cgcttgagct cggcgtcgag ctcggccggc
     7501 gtgctgccga cgggcgtgag cgcgagctga tcgagccggg cgaggacgtc ggggaatttc
     7561 agcgccgcgc gcgactcgtc ggcgatcttc gcgcacacct cggacggcgt tcccgcgggc
     7621 gcgaagaacg cccaccacac gtcgaaggtc tggaactggg gcagcgtctc gaccaccgcc
     7681 ggcacgtcgg gcatgcgctc ggtgcgcttc gtcgcgagca cggcgagcgg tcgcaccttc
     7741 cccaggttcg gcgcgaggaa gccgtacgcg gggaagtaga gcggcacgcg ccccgagacc
     7801 cagtcgttga tcatctgccc gaagttcgcc tgcgcgtacg gcacgtgcag catgtcgatg
     7861 ccggcgtaga gcttgagcga ctcgcccgcg aggtggaaca cggagcccgc gccggtcgag
     7921 gcgtactcga tcttgccggg gtgcttcttc gcgtagtcga tcagctcggc gagcgtatgg
     7981 atcggcgtcg tgctgctgat cgcgacgtag gtcggcgagc gcacggtgag gctgatcggg
     8041 acgaagtccc tgaggatgtc gaacgacacg ttcttgaaca ggaaccgcgc gttgacgtac
     8101 gtgcccgagg tgccgaaggt gatggtgtgg ccgtctggcg ccgcgcgcgc gagcgcctgc
     8161 gccatctgcg ccccgcgcgc ggcggggcgc acgtcgacga tgacgggatg gcccatcgcc
     8221 gccgccatct tcgccgcgac catgcgcagc gccacgtcgc cgctcgagcc gggcgacgcg
     8281 gtgctgatca cctggatggg cttcgtgggg aacggagcct ggctccacgc cagcgtgggc
     8341 gcgagacagg cggcgcagag gatcgatcga accggcattt tggaaacgag ggtaccatac
     8401 cgcccatgcg ggcgatcaac acccacttcc actggtatcc ccggtcggtc ttcgaggcgc
     8461 tggcgaagcg cggcgatttc ccgagggcgg tcgccaccgc gcgcggcggc tacatggtct
     8521 cgccgcacga gggcgcggcg cgcaccgaca cctgggccga ctggttcgac ctcgacgcga
     8581 tgctcggcac gatggacgcg gccggggcga agatcggctg cgacttcggc gtcgtctgca
     8641 ccaccggccc cttcggcgcc ttcttctcgg agacggacgc ggcgcacggt cttgcgttcg
     8701 cgcaagcatg gaacgacgag atggcgggcg cggtcgcgcg caacccgggc cgcgtctggg
     8761 cgagcggcgt cgtgccgctg caggacccga agaaggcggt cgacgagctc gagcgcgcga
     8821 tgaggctcgg cctcgtcggc gtcaacgtgc cgagctcgat cgggccgcgg ggcgtgacgc
     8881 gcatcgacga cccctcgctc gagg
//
LOCUS       DATDMW010000195         4475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_432699, whole genome shotgun sequence.
ACCESSION   DATDMW010000195 DATDMW010000000
VERSION     DATDMW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432699"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VI319_08490"
     CDS             <1..877
                     /locus_tag="VI319_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEY6863924.1"
                     /translation="ELAALADRVRAEGGAVIGSYLDPLGGRPLALAVLPIDKIEPTPF
                     QRDLSDAHHKRLSEVIHKTGRFLDPVIAITAPEKTGGFWTPNGRHRLEAMRRLGARSI
                     TALVVPKREVAWQILALNTEKAHNLREKSLEVIRITRGLLDDPADARRPETDFAFYLE
                     EASLVTLGVCYEKNGKFAGGAYNPIVRRLEKFSDEPLSKAIRAHEKHAEMRADLDARV
                     AEVIAKLKDRGFVSPYLRAFVVARINPLRWIQGEPPPLEEVLKTMRERAGKFNVDKVK
                     QEDLAGAGGAPDEAE"
     gene            complement(890..1750)
                     /locus_tag="VI319_08495"
     CDS             complement(890..1750)
                     /locus_tag="VI319_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6863925.1"
                     /translation="MKPGIEAFFDPVTATMSYVVYDAPGGRAAVVDSVLDFDPKSGRT
                     STASADKLLEFVSAQRLTLDWILETHAHADHLSAARYLKRRAGGRIAIGERIRQVQGA
                     FKKIFNLEPEFRLDGSQFDHLFSEDETFRIGELEGRVLFVPGHTPADVAYHVGDAVFV
                     GDTIFMPDVGSARCDFPGGNAHALYRSVRRLLALAGETRIFVCHDYPPAGRGPRAETT
                     VREQRLANIHLRDGVGEEPFVAMRTARDATLDMPALLLPSVQVNVRAGEMPPAEANGV
                     TYLKIPINAL"
     gene            1862..2143
                     /locus_tag="VI319_08500"
     CDS             1862..2143
                     /locus_tag="VI319_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY6863926.1"
                     /translation="MRAAVGEAAAALRVLANENRLLLLCELSQGERSVGELEETLDIR
                     QPSLSQQLGVLRAVGLVSTRRNGKFIHYSVADRKALALLDTLYHLYCRS"
     gene            2149..2583
                     /locus_tag="VI319_08505"
     CDS             2149..2583
                     /locus_tag="VI319_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEY6863927.1"
                     /translation="MSIDWNAFTPSQSLAGGAMIGAATALFFMLNGRIAGISGIIGGL
                     LRPQAGDISWRLTFVFGMLLAPLAYAAFAALPEIRIAGGYPTLALAGLLVGLGTRYGS
                     GCTSGHGVCGVARLSPRSLAATLAFMAAGFATVFAVRHLAIV"
     gene            2594..3031
                     /locus_tag="VI319_08510"
     CDS             2594..3031
                     /locus_tag="VI319_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEY6863928.1"
                     /translation="MYSFASFAAGLLFGAGLIVAGMANPAKVLGFLDVAGSWDPSLAL
                     VMAGAVGVGAAAFALMRRRRHTLIGAPLRLPGARKVDRRLVIGSLVFGVGWGLAGICP
                     GPALVLAGQASAKGLVFLAAMLAGNVLFELFEKKRERKARTRA"
     gene            3122..3343
                     /locus_tag="VI319_08515"
     CDS             3122..3343
                     /locus_tag="VI319_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863929.1"
                     /translation="MTDITHSLSALIKRSLLFYVGIILICFAIFPIVFLYGLSLVIEK
                     FVGRENAARIERFWDTIGKPYEYLTRAAR"
     gene            complement(3398..3934)
                     /locus_tag="VI319_08520"
     CDS             complement(3398..3934)
                     /locus_tag="VI319_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY6863930.1"
                     /translation="MERSTYGTTAKLLHWLIAAILLVQLALGWLMPEVRRGMSPGTAM
                     NAHMSIGVIALALIVARLAWRVTHPVEPDPALRGWQRASSEAVHWLLYALVLVATITG
                     WFYASMRGWTVTVFSLFPLPALTPEGSQLGRAIGRLHESATAILAAAIGAHVAAALVH
                     LFVYRDRVMQRMLPSIRK"
     gene            4092..>4475
                     /locus_tag="VI319_08525"
     CDS             4092..>4475
                     /locus_tag="VI319_08525"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY6863931.1"
                     /translation="MHRSAGLFALAAAVAAALIAACAGTGPGAAPGANDIAGTVSGPS
                     GPEAGVWVIAETTELPTKYAKVVVTDDAGRYVIPELPKAEYSVWVRGYGLVDSPKVSA
                     HPGQTLALTATVAPSAKAAAEYYPGM"
BASE COUNT          699 a         1534 c         1580 g          662 t
ORIGIN      
        1 ggagctcgcc gcgctcgccg atcgcgttcg agccgagggg ggcgccgtca tcggcagcta
       61 cctcgacccg ctcggcggtc gtccgctcgc gctcgccgtc ctgccgatcg acaagatcga
      121 gccgacgccg ttccagcgcg acctgtccga cgcgcaccac aagcgcctct ccgaggtgat
      181 ccacaagacc gggcgcttcc tcgatccggt catcgcgatc acggctccgg agaagacggg
      241 cgggttctgg acgccgaacg gccgccaccg cctcgaggcg atgcgccggc tcggcgcacg
      301 ctcgatcacg gcgctggtcg tgccgaagcg cgaggtggcg tggcagatcc tcgcgctcaa
      361 caccgagaag gcgcacaacc tgcgcgagaa gtcgctcgaa gtcatccgca tcacgcgcgg
      421 gctgctcgac gatccggccg acgcgcggcg ccccgagacc gacttcgcgt tctacctcga
      481 ggaggcgtcg ctcgtcaccc tcggcgtctg ctacgagaag aacggcaagt tcgcgggcgg
      541 cgcctacaac ccgatcgtgc ggcgcctgga gaagttctcc gacgagccgc tgtcgaaggc
      601 gatccgggcg cacgaaaagc acgccgagat gcgcgccgac ctcgatgcgc gcgtcgccga
      661 ggtgatcgcg aagctgaagg accgcggctt cgtcagcccc tacctgcgcg cgttcgtcgt
      721 cgcgcgcatc aatccgcttc gctggatcca gggcgagccg ccgccgctcg aggaggtgct
      781 gaagaccatg cgcgagcgtg ccggcaagtt caacgtcgac aaggtgaagc aggaggacct
      841 cgccggcgcg ggcggcgcgc ccgacgaggc ggaataaggg tccgacgagc tagagcgcgt
      901 tgatgggaat cttgaggtag gtgacgccgt tcgcctcggc gggcggcatc tccccggcgc
      961 gcacgttcac ctgcaccgag ggcagcagca gggccggcat gtcgagcgtg gcgtcgcgcg
     1021 cggtccgcat cgcgacgaac ggttcctcgc cgacgccgtc gcgcagatgg atgttcgcga
     1081 gccgctgctc gcgcacggtc gtctccgcgc gcggcccgcg gccggcgggc gggtagtcgt
     1141 ggcacacgaa gatgcgggtc tcgcccgcga gcgcgagcaa ccggcgcacc gagcggtaca
     1201 gcgcgtgcgc gttgccgcca ggaaagtcgc agcgcgccga gcccacgtcg ggcatgaaga
     1261 tggtgtcgcc gacgaagacg gcgtccccga cgtggtaggc gacgtccgcg ggcgtgtgtc
     1321 ccggcacgaa cagcacccgg ccctcgagct ccccgatgcg gaaggtctcg tcctcggaga
     1381 agagatggtc gaactgcgat ccgtccagcc ggaactcggg ctcgaggttg aagatcttct
     1441 tgaacgcgcc ctgcacctgg cggatgcgct ccccgatcgc gatgcggccc ccggcgcgcc
     1501 gcttcagata gcgcgccgcc gataggtggt cggcatgcgc gtgcgtctcc aggatccagt
     1561 cgagcgtcag ccgctgcgcc gagacgaact cgagcagctt gtcggccgag gcggtcgagg
     1621 tgcgccccga cttggggtcg aagtcgagca ccgagtccac caccgcggcg cggccgcccg
     1681 gggcgtcgta cacgacgtag gacatcgtcg cagtgacggg atcgaagaac gcctcgatgc
     1741 cgggtttcat gggtctccct cttgagagac aatatataga atgatataat gtaagtcaat
     1801 aaaacgtttc cggtgaggcg cgatgagcgt ggcggcgaaa cccagattga gcacgacgcg
     1861 catgcgggcg gcggtggggg aggcggccgc ggcgctgcgg gtgctggcga acgagaaccg
     1921 gctgctcctc ctgtgcgagc tcagccaggg cgagaggtcg gtgggcgagc tcgaggagac
     1981 gctcgacatc cggcagccct cgctgtcgca gcagctcggc gtgctgcgtg ccgtggggct
     2041 cgtgagcacg cgccggaacg gaaaattcat ccattactcg gtggccgacc gcaaggcgct
     2101 cgcgctgctc gatacgcttt atcacctcta ctgcaggagc tgagcatcat gtcgatcgac
     2161 tggaacgctt tcacgccgtc gcagtcgctc gccggcggcg cgatgatcgg cgccgcgacg
     2221 gcgctcttct tcatgctcaa tgggcgcatc gccggcatca gcggcatcat cggcggcctg
     2281 ctgcggccgc aggcgggcga catctcgtgg cggctgacgt tcgtattcgg gatgctactc
     2341 gcgccgctgg cgtacgccgc gttcgcggcg ctgcccgaga tccggatcgc gggcgggtac
     2401 cccacactcg cgctcgccgg gctgctggtc ggcctcggca cgcgctacgg ctcgggctgc
     2461 accagcggcc acggggtgtg cggcgtcgcg cggctgtcgc cgcgctcgct ggccgcgacg
     2521 ctcgcgttca tggcggccgg gttcgcgacg gtgttcgcgg tgcgccacct cgcgatcgtc
     2581 tgagaggccc ctcatgtaca gcttcgcttc cttcgccgcc ggccttctgt tcggggccgg
     2641 cctgatcgtc gccggcatgg cgaatcccgc caaggtcctc ggcttcctcg acgtcgcggg
     2701 atcgtgggat ccgtcgctcg cgctggtgat ggccggcgcg gtcggcgtcg gcgccgccgc
     2761 cttcgcgctg atgcgcaggc gcaggcacac gctgatcggc gcgccgctgc ggcttcccgg
     2821 cgcgcgcaag gtcgaccgcc gcctcgtgat cggcagcctc gtgttcgggg tcggctgggg
     2881 gctcgcgggg atctgccccg gacccgcgct ggtgctcgcc gggcaggctt cggcgaaggg
     2941 actcgtgttc cttgccgcga tgctggcggg caatgtgctg ttcgagctat tcgagaagaa
     3001 gcgcgagcga aaggcgcgga cgcgggcgta ggccgctcgg acggcacgcg gggaaacgcg
     3061 aataccgcga gcgtttcccc taatagccgg aggagggcat cggtggtacg gtcgatgggc
     3121 catgaccgac atcacgcact ccctaagcgc cctcatcaag cgctcgctgc ttttctacgt
     3181 gggcatcata ctcatctgct tcgcgatctt cccgatcgtc ttcctgtacg gactctcgct
     3241 cgtcatcgag aagttcgtcg gccgcgagaa cgcggcgagg atcgagcgat tctgggacac
     3301 gatcggcaag ccgtacgagt atctgacccg cgccgcgcgc tagccgctac aggacctcga
     3361 ggcccgaccc gcccgccgga aggcgcggcg gacgatttca tttccggatt gacggcagca
     3421 tgcgctgcat cacgcggtcg cggtaaacaa agaggtggac gagcgcggcc gcgacgtgcg
     3481 cgccgatcgc ggccgccagg atcgccgtgg cgctctcgtg cagccgcccg atcgcgcggc
     3541 cgagctgcga gccttcgggc gtgagcgcgg gcagcgggaa cagcgagaac accgtcacgg
     3601 tccagccgcg catcgacgcg tagaaccagc cggtgatcgt cgccacgagc acgagcgcgt
     3661 agagcagcca gtgcaccgcc tcggaagacg cgcgctgcca gccgcgcagg gcggggtcgg
     3721 gctcgaccgg atgcgtcacg cgccacgcga gccgagcgac gatcagcgcc agtgcgatca
     3781 cgccgatcga catgtgcgcg ttcatcgcgg tgcccggcga catcccgcgc ctgacctcgg
     3841 gcatcagcca gccgagcgcg agctgcacga gcaggatcgc ggcgatcagc cagtgaagga
     3901 gtttcgcggt cgtgccgtac gtggagcgct ccatgacgcg cacgataccg cacggaggcc
     3961 cggcgccgcc cggacacacg ttgcttgcgc gatcaacacg gaccgtgttg ccggcattcg
     4021 gggaaaggtg cattctcttg cgcggaaacc cgatccgcca tccaagcccg cacgactccg
     4081 agggggaaca catgcacaga tccgcaggcc ttttcgcgct cgccgccgcc gtcgcagccg
     4141 cgctgatcgc cgcgtgcgcc ggcaccggcc cgggcgccgc tccgggcgcc aacgacatcg
     4201 ccggaaccgt gagcgggccc tccggccccg aagccggcgt gtgggtgatc gccgagacga
     4261 ccgagctgcc cacgaaatac gcgaaggtgg tcgtcacgga cgacgccggt cgctacgtga
     4321 tccccgagct gcccaaagcc gagtacagcg tctgggtccg cggctacggc ctggtcgatt
     4381 cgcccaaggt gagcgcgcat cccggacaga cgctggcgct gaccgcgacg gtcgcgccga
     4441 gcgcgaaggc cgcggccgag tactaccccg ggatg
//
LOCUS       DATDMW010000196         5879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2428092, whole genome shotgun sequence.
ACCESSION   DATDMW010000196 DATDMW010000000
VERSION     DATDMW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428092"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="VI319_08530"
     CDS             complement(<1..1720)
                     /locus_tag="VI319_08530"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY6863932.1"
                     /translation="MNAATASTNPLLDFSGLPQYGAVRPEHVAPAIARLLAENRGLVG
                     RLVAESTPATWDDFVRPLEEANERLARAWGQVAHLHAVDDNPAIRDAYNASLPKVTQY
                     WTELAQHAGLFAKYKALAGSPAFASLETAQRRIVENELRDFRLGGAELAEDEKREFAA
                     IQEELAALAAKFSENLLDATNAFSLFVDERGRLSGIPDDALRAAAESARKDGKAGWKL
                     TLHAPSYLPVMQYADDRGLRETLYREYVTRASEFGAPGLDNTEIIRRELVLRRQKARL
                     LGYESFAEYSLVPKMAESPREVLRFLDDLAERALPFARKDYAELQSFAKSELGLIEVC
                     AWDVAWASEKLRAKKYAFSDQEVKQYFPEPKVLEGMFRVVQTIYGVRIEPDSAPVWHP
                     DVRFFRITDRSGALIGRFYVDLYARETKRGGAWMDEAITRHRTKAGVQAPVAYLICNF
                     SGPLEGPDGRRRPALFTHDEVSTLFHEFGHGLHHLLTRVDYLGASGIRGVEWDAVELP
                     SQFMENFCWEWEVLQHMSAHVDTGQPLPRPLFDKMLAAKNFQSGLATLRQIEFALFDM
                     LLHTEHD"
     gene            complement(1756..2607)
                     /gene="folD"
                     /locus_tag="VI319_08535"
     CDS             complement(1756..2607)
                     /gene="folD"
                     /locus_tag="VI319_08535"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY6863933.1"
                     /translation="MQTRIIDGNALAREARAALRMEVDALSAAGRRPGLAVVLAGDDP
                     ASRVYVRNKARACEETGVLSRQIDLPADVSEAALLGQVERLNADPAIHGILVQLPLPR
                     GVSSERVVRAIAPEKDVDGFHPCNMGLLAEGRPRFVPCTPKAVMAILDREGIALEGKR
                     AVVVGRSNIVGKPVALLLLQRGATVTVCHSKTADLEEVTRQADVLVVAVGRPNLVTGR
                     AVKSGAVVIDVGINRLPGGRLVGDVDASSVTGVAARLTPVPGGVGPMTITMLIANTVL
                     AARSVHD"
     gene            2754..5432
                     /gene="aceE"
                     /locus_tag="VI319_08540"
     CDS             2754..5432
                     /gene="aceE"
                     /locus_tag="VI319_08540"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY6863934.1"
                     /translation="MSALPQFTAANDPDAQETQEWLDALEAVLEHEGPERAHYLLEKL
                     VEKARRSGAYLPYTAQTAYINTIPPHLEERSPGDHALEERIRSYCRWNAMVMVARANK
                     NDDELGGHIASFASVSTLFDVGFNHFWRAPSAEHGGDLVYFQGHSAPGIYARALLEGR
                     LTEEQLNNFRREVDGKGVSSYPHPWLMPEFWQFPTVSMGLGPIQGIYMARFLKYLQAR
                     GLANTEGRKVWVFCGDGEMDEPESLGAIGLAAREKLDNLIFIVNCNLQRLDGPVRGNG
                     KIIQELEGDFRGSGWNVIKLIWGSYWDPLLARDKEGRLLKVMEDSVDGEYQNYKANDG
                     AFVRKYFFGKDSKLLEMVSRMTDDDIWRLNRGGHDPHKIYAAYAAATKHQGQPSVILA
                     KTIKGYGMGKIGEAKMATHQQKKMDLESIRQFRDRFSIPIADDQLEKLPFYIPAADSP
                     EMQYLHERRRALGGYLPQRRRRADTTPEVPPLSAFEALLKATDREISTTMAFVRVLTA
                     LVRDKNLGRHVVPIVPDEARTFGMEGMFRQLGIYSSEGQKYEPVDRGQVMYYREDKAG
                     QILEEGITEAGSFCSWIAASTSYSHSNQAMVPFFVFYSMFGFQRVGDLAWAAGDQRAR
                     GFLLGGTSGRTTLNGEGLQHEDGHSHALSSVIPNCVSYDPTFAYELAVIVHDGLRRMV
                     ANQEDVFFYITVMNENYTHPAMPEGAAEGILKGMYLLRPRGKKKHRAQLLGSGTILRE
                     VIAAAELLEADWDVGADVWSATSFTELRREGQAAERWNMLHADAKPRIPYVEQCLAKH
                     EGPVVAASDYVRTFADQIRPYVPRDRVYTVLGTDGFGRSDSRAKLREFFEVNRYYVAV
                     AALKALADEGTITPAVVAQAIKKYGLDPEKPNPMTV"
     gene            5462..>5879
                     /locus_tag="VI319_08545"
     CDS             5462..>5879
                     /locus_tag="VI319_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY6863935.1"
                     /translation="MGAIKEVLVPDIGDFKDVDVIEVLVKPGDSVQPEQSLITVESDK
                     ATMEIPSPEAGKVKELRVKVGDKVSQGRPILVLEAGDGAAAKAQQPAPPKPASAAQPA
                     KPAPAAQPAKAAPPPAAAPRAASGGPREVAVPDIGDF"
BASE COUNT          962 a         2033 c         2000 g          884 t
ORIGIN      
        1 gatcgtgctc ggtgtgcagc aacatgtcga acagtgcgaa ctcgatctgg cgcagcgtgg
       61 caaggccgct ctggaagttc ttcgcggcga gcatcttgtc gaacagcggg cgcggcagcg
      121 gctgcccggt gtcgacgtgc gcgctcatgt gctgcagcac ctcccattcc cagcagaagt
      181 tctccatgaa ctgcgaaggc agctcgaccg cgtcccattc gacgccgcgg atgcccgagg
      241 cgcccaggta gtcgacgcgg gtgagcaggt gatgcaggcc gtggccgaac tcgtggaaca
      301 gcgtgctgac ttcgtcgtgg gtgaacagcg ccggccgcct gcgtccgtcg ggaccctcga
      361 gcggcccgga gaaattgcag atcaggtacg cgaccggagc ctgcacgccg gccttcgtgc
      421 gatgccgcgt gatcgcctcg tccatccagg cgccgccgcg cttggtctcg cgcgcgtaca
      481 ggtcgacgta gaacctgccg atcagggcgc ccgagcggtc cgtgatgcgg aagaagcgca
      541 cgtcgggatg ccacacgggc gcgctgtccg gctcgatgcg cacgccgtag atcgtctgca
      601 cgacgcggaa catgccctcg aggaccttgg gctcggggaa gtactgcttc acctcctgat
      661 ccgagaacgc gtacttcttg gcgcgcagct tctcggatgc ccaggcgacg tcccaggcgc
      721 acacctcgat cagtcccagc tcgctcttcg cgaacgactg aagctcggcg tagtccttgc
      781 gcgcgaaggg cagcgcgcgc tcggcgagat cgtcgaggaa gcgcagcacc tcgcgcggcg
      841 actcggccat cttcggaacg agcgagtact cggcgaagct ctcgtagccg agcagccgcg
      901 ccttctgccg gcgcagtacg agctcgcgcc ggatgatttc ggtattgtcg agcccgggtg
      961 ctccgaactc gctcgcgcgc gtcacgtact cgcggtacaa cgtctcgcgc aggccgcggt
     1021 cgtcggcgta ctgcatgacc ggcagatacg acggcgcgtg cagcgtgagc ttccagccgg
     1081 ccttgccgtc cttgcgcgcg gactcggctg cggcccgcag cgcgtcgtcg gggatgcccg
     1141 aaagcctccc gcgctcgtcc acgaacagcg agaacgcatt ggtcgcgtcg agcaggttct
     1201 ccgagaactt cgcggcgagc gcggccagct cctcctggat cgcggcgaac tcgcgcttct
     1261 cgtcctcggc cagctcggcg cccccgagcc ggaagtcgcg tagctcgttc tccacgatgc
     1321 gccgctgcgc ggtctcgagc gacgcgaacg ccggggaccc cgcgagcgcc ttgtacttcg
     1381 cgaacagccc ggcgtgctgc gcgagctcgg tccagtactg cgtgaccttc ggcaggctcg
     1441 cgttgtaggc gtcgcggatc gccgggttgt cgtcgacggc gtgcaggtgc gcgacctggc
     1501 cccaggcgcg cgcgagacgt tcgttcgcct cctcgagcgg tcgcacgaag tcgtcccagg
     1561 tcgcgggcgt gctctcggcc accagccgac ccaccagccc gcgattctcg gcgagcagcc
     1621 gcgcgatcgc cggcgcgacg tgctccggcc ggacggcgcc gtactgcgga aggcccgaaa
     1681 aatcgagcaa ggggttggtg gaggctgtgg ctgcgttcac cgcttgttgt tgcatgtcgc
     1741 gtttccccgg agtgttcaat cgtgcaccga acgcgcggcg agcacggtgt tcgcgatcag
     1801 catcgtgatg gtcatcgggc ccacgccgcc cggcaccggc gtcagacgcg ccgcgacgcc
     1861 ggtcacgctg ctcgcgtcga cgtcgccgac gagccggccg ccgggcaggc gattgatacc
     1921 cacgtcgatc accacggcac ccgatttgac cgcgcgaccc gtgacgaggt tcggccggcc
     1981 gaccgcgacg accagcacgt ccgcctgccg cgtcacctcc tccaggtcgg cggtcttgga
     2041 atggcacacc gtcaccgtcg cgccgcgctg gagcagcagc agcgcgaccg gcttgccgac
     2101 gatattgctg cgtcccacga cgacggcgcg cttgccttcg agcgcgatgc cttcgcggtc
     2161 gaggatcgcc atcacggcct tcggcgtgca gggcacgaag cgcggacggc cttccgcgag
     2221 cagccccatg ttgcagggat ggaagccgtc gacgtccttc tccggcgcga tcgccctcac
     2281 cacgcgctcg ctcgagacgc cgcgcggcag cggcagctgc accaggatgc cgtggatcgc
     2341 ggggtcggcg ttgagccgct cgacctgccc gagcagcgcc gcctcgctga cgtccgccgg
     2401 cagatcgatc tggcgcgaca gcacgccggt ttcttcgcac gcgcgcgcct tgttgcgtac
     2461 gtacactcgg gaggcgggat cgtctccggc gagcacgacc gccaggcccg gccggcgccc
     2521 cgccgcggac agggcgtcga cctccatgcg cagcgccgcc cgggcctcgc gcgcgagggc
     2581 attgccgtcg atgattcgcg tctgcatggg agcgcttcgg accggaagca ggggaaggcg
     2641 agcgcattct agcatttgct ccgggcggct caggcggggc gctcggggct tttgtataat
     2701 gaaaacgccg cacgccccga tcccgtcccg cccatgcctt gagagggaac accatgtccg
     2761 cgctgccgca gttcaccgct gcgaacgatc ccgacgccca ggaaacccag gagtggctcg
     2821 acgcgctgga agcggtgctc gagcacgagg ggcccgagcg cgcgcattac ctgctcgaga
     2881 agctggtcga aaaggcgcgg cgctcgggcg cctacctgcc ctacaccgcg cagaccgcgt
     2941 acatcaacac gatcccgccg cacctcgagg agcgttcgcc cggcgaccac gcgctcgagg
     3001 agcgcatccg ctcgtactgc cgctggaatg cgatggtgat ggtcgcgcgc gcgaacaaga
     3061 acgacgacga gctcggcggc cacatcgcga gcttcgcgtc ggtcagcacc ctgttcgacg
     3121 tcggcttcaa ccatttctgg cgtgcgccgt cggccgagca tggcggcgat ctggtgtact
     3181 tccagggcca ctcggcgccg ggcatctacg cgcgcgccct gctcgagggc cggctcaccg
     3241 aggagcagct gaacaacttc cgccgcgagg tggacggcaa gggcgtctcg tcctatccgc
     3301 atccctggct gatgcccgag ttctggcagt tcccgaccgt gtcgatgggc ctgggcccga
     3361 tccagggcat ctacatggcg aggttcctca aatacctgca ggcgcgcggc ctcgcgaaca
     3421 ccgagggccg caaggtctgg gtgttctgcg gcgacggcga gatggacgag cccgagtcgc
     3481 tgggcgcgat cgggctcgcc gcgcgcgaga agctcgacaa cctgatcttc atcgtgaact
     3541 gcaacctgca gcgcctcgac ggcccggtgc gcggcaacgg caagatcatc caggagctcg
     3601 aaggcgattt ccgcggctcg ggctggaacg tgatcaagct gatctggggc tcgtactggg
     3661 acccgctgct cgcgcgcgac aaggagggcc ggctgctcaa ggtcatggag gactccgtcg
     3721 acggcgagta ccagaactac aaggcgaacg acggcgcctt cgtgcgcaaa tacttcttcg
     3781 gcaaggactc gaagctgctc gagatggtct cgcgcatgac cgacgacgac atctggcgcc
     3841 tgaaccgcgg cgggcacgat ccgcacaaga tctacgccgc ctacgccgcg gcgaccaagc
     3901 accagggcca gcccagcgtc atcctggcga agacgatcaa gggctacggc atgggcaaga
     3961 tcggcgaggc caagatggcc acgcaccagc agaagaagat ggatctcgag tcgatccgcc
     4021 agttccgcga ccgcttcagc atcccgatcg cggacgacca gctcgagaag ctgccgttct
     4081 atattcccgc ggccgactcg ccggagatgc aatatctgca cgagcgccgg cgtgcgctcg
     4141 gcggctacct gccgcaacgc aggcggcggg cggacaccac gcccgaggtc ccgccgctct
     4201 ccgcgttcga ggcgctgctc aaggcgaccg accgcgagat ctcgaccacc atggcgttcg
     4261 tgcgcgtgct gaccgcgctc gtgcgggaca agaacctcgg ccggcatgtc gtgccgatcg
     4321 ttccggacga ggcgcgcacc ttcggcatgg aaggcatgtt ccgccagctc gggatctact
     4381 cctcggaagg gcagaagtac gagcccgtcg acagaggcca ggtgatgtac taccgcgagg
     4441 acaaggccgg tcagatcctc gaggaaggca tcaccgaagc gggctcgttc tgctcctgga
     4501 tcgcggcgtc gacctcttac agccactcga accaggcgat ggtgcccttc ttcgtcttct
     4561 actcgatgtt cggcttccag cgcgtgggcg acctcgcgtg ggcggccgga gaccagcgcg
     4621 cccgcggctt cctcctgggc gggacctcgg gacgcaccac gctgaacggc gagggactcc
     4681 agcacgagga cggtcactcg catgcgttgt cgtcggtgat cccgaattgc gtgtcctacg
     4741 atccgacctt cgcctacgag ctggcggtca tcgttcacga cgggctgcgg cgcatggtcg
     4801 ccaaccagga agacgtgttc ttctacatca ccgtgatgaa cgagaactac acgcacccgg
     4861 cgatgcccga gggcgccgcc gaaggcatcc tgaagggcat gtacctgctg cgcccgcgcg
     4921 gcaagaagaa gcatcgcgcg cagctcctcg gctcgggcac catcctgcgc gaggtgatcg
     4981 ccgcggccga gctgctcgag gccgactggg acgtgggcgc cgacgtgtgg agcgcgacca
     5041 gcttcaccga gctgcgtcgc gaggggcagg cggcggagcg atggaacatg ctgcacgcgg
     5101 atgcgaagcc gcggattccc tacgtcgagc aatgcctcgc caagcacgag gggccggtgg
     5161 tcgccgcgag cgattacgtg aggaccttcg ccgaccagat ccggccgtac gttccgcgcg
     5221 accgcgtcta caccgtcctc ggcacggacg gcttcgggcg ctccgactcg cgcgcgaagc
     5281 tgcgcgaatt cttcgaggtg aaccgctatt acgtggccgt ggccgcgctg aaggcgctgg
     5341 cggacgaggg gacgatcacc ccggccgtgg tggcgcaggc gatcaagaag tacggcctcg
     5401 acccggagaa gcccaacccg atgacggtct gacccgtccg cgcggacacg aacgacaaca
     5461 catgggcgcg atcaaggaag tcctggtccc cgacatcggc gatttcaagg acgtcgacgt
     5521 catcgaggtg ctggtgaagc ccggcgactc ggtgcagccg gagcagtcgc tgatcacggt
     5581 cgagtccgac aaggcgacga tggagatccc gtcgcccgag gccgggaagg tgaaggagct
     5641 gcgcgtcaag gtcggcgaca aggtgtcgca gggccggccg atcctcgtgc tcgaggcggg
     5701 cgacggcgcc gcagcgaagg cgcagcagcc ggcgccgccg aagcccgcat cggccgccca
     5761 gccggccaag cccgccccgg ccgctcagcc ggcgaaggcc gcgccgccgc ccgccgctgc
     5821 gccgcgcgcc gcctccggcg gaccgcgcga ggtcgcggtt cccgacatcg gcgacttca
//
LOCUS       DATDMW010000197         4448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1430105, whole genome shotgun sequence.
ACCESSION   DATDMW010000197 DATDMW010000000
VERSION     DATDMW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430105"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2336)
                     /locus_tag="VI319_08550"
     CDS             complement(<1..2336)
                     /locus_tag="VI319_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY6863936.1"
                     /translation="MSARGLALLALSALSVGAALAAPVADIRNTKHNLSAAGPGTVKA
                     TSESQLCVFCHTPHGATPALTPLWNRAVSGGTYSVYTSSSLDANAIQGSLDQPGGSSK
                     LCLSCHDGTLAIGNVNVLAGQGSVSTQGSVSVPMTGVGAGGTMPTGAGTTSGATRNLG
                     VDLTNDHPVSVSFTSALAARDGELRPVDATQKWPPGSGTAVGIPSPGYKPLLPLEPTG
                     VGRTGQVQCATCHDPHLRETDPSKGNQKFLRANRFQEAPPSNVYDPANDIVCLSCHDK
                     NAGSGAWAYSAHANPLVATETYTVTAAGTREFPSSQPVWKAACLNCHDTHTVQGARRL
                     LREGTDSPATPKQGGASAIEQTCYQCHTSAATTAITPGTTVPNIQGDFALPRHMPIAS
                     TEQAAGAEVHDIGGNFGDASFVDCTSPTNKCGADFVEQRTKLGVANLSNRHAECTDCH
                     NPHRVVKFRSVVGTPAGSISGAPDAAGTHPHTDTAGTTHTNIASGVLRGAWGVEPVYG
                     SAAFQNAPISYLVKRGDPGASADTSVGAAYVTREYQVCLKCHSDYGYSDNNIYPTGNR
                     PPLGPPGTPSGTNNLTQYTNQAKELQAPFTHQGEVSTADSGAAAAYSLNNHRAWHPVM
                     GQTGRTPPVRGGLAANNWNFPWRNAVGTQTMYCTDCHGSNVSSATSVIPDGGENGNPW
                     GAHGSANNFLLKGVWNTSTGTGQQTNGLCFKCHSYTVYATNSGTRTGFFLADIGKDGH
                     THHADKIGRLRCNWCHVAVPHGWKNKAFLVNLNDVGAEA"
     gene            complement(2326..3402)
                     /locus_tag="VI319_08555"
     CDS             complement(2326..3402)
                     /locus_tag="VI319_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEY6863937.1"
                     /translation="MTARAFAILLAAALAACAGPGGRALYYGVEDVPEAQRPIWPAAP
                     ETPRYLYAGQLVGEQNFRASEPAPGLQRVLAAIAGIVAGERAPVVLQRPQAGVVDDAG
                     RVYVTDASRQAVFVFDARAGELAVWDRADGLTGFVAPVGVALGPAGELLVADAELGIV
                     ARLDARGATLPPFGRGVLKRPTGLAYDPIGGRIYVADTYAHDVKVFDADGRLLATLGR
                     RGSGEGEFNYPTHLAFARGELYVTDTLNSRIEVFRTDGTGLVRTFGARGLYVGNLVRP
                     KGVAVDSEGNVYVVESYHDHLLVFDRNGAFLMAIGGVGQDAGRFYLPAGVWVDAKNRV
                     FVADMFNGRVAVFQFLGGEVDSER"
     gene            complement(3405..3794)
                     /locus_tag="VI319_08560"
     CDS             complement(3405..3794)
                     /locus_tag="VI319_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HEY6863938.1"
                     /translation="MRIARILLAIAAAASLAALVEIGPAQAEYGDVVLNKRSDREGMR
                     PVVFPHWFHRIRFRCKVCHAALGFKRRAGANDVQMADINDGRFCGMCHNGQIAWAADR
                     CDLCHSGRPGLPSGIYGGDRTLGPGRW"
     gene            complement(3798..4334)
                     /locus_tag="VI319_08565"
     CDS             complement(3798..4334)
                     /locus_tag="VI319_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HEY6863939.1"
                     /translation="MRKRRTARWVLAALLLAAQATRAGDWLPLARDGVHDPSSPSLQL
                     LQEPREALSGLAPDTAGNQVRWVEALDKGQISPRTNILPETKVNVLDLDVLMNLRGGT
                     PIVRFPHRQHTLWLDCSNCHEHLFKSKAGANKLSMLQILQGEQCGVCHGAVSFPLTEC
                     TRCHSVPRGTAASQPAKP"
BASE COUNT          570 a         1578 c         1627 g          673 t
ORIGIN      
        1 gcctcggcgc cgacgtcgtt caggttcacg aggaacgctt tgttcttcca gccgtgcggc
       61 accgcgacgt ggcaccagtt gcagcgcagc cgcccgatct tgtcggcgtg gtgcgtgtgg
      121 ccgtccttgc cgatgtcggc gaggaagaag ccggtgcgcg tccccgagtt ggtcgcgtac
      181 acggtgtagc tgtggcactt gaagcacagg ccgttcgtct gctgcccggt gccggtcgac
      241 gtgttccaca cgcccttcag caggaagttg ttggccgagc cgtgcgctcc ccagggattg
      301 ccgttctcgc cgccgtcggg gatcaccgag gtcgccgacg acacgttcga gccgtggcag
      361 tcggtgcagt acatcgtctg cgtgccgacg gcgttgcgcc acgggaaatt ccagttgttc
      421 gccgcgagtc cgccgcgcac cggcggcgtg cggccggtct gccccatcac cgggtgccag
      481 gcgcggtggt tgttgagcga gtacgcggca gccgcgcccg agtcggcggt cgaaacctcg
      541 ccctggtggg tgaacggcgc ctggagctcc ttcgcctggt tcgtatactg cgtcaggttg
      601 ttcgtgcccg agggcgtgcc gggcggcccg agcggcggcc ggttcccggt cgggtagatg
      661 ttgttgtcgc tgtagccgta gtcggagtgg cacttgaggc acacctggta ctcgcgcgtg
      721 acgtacgcgg cgccgacgct cgtgtcggcg ctggcgcccg ggtcgccgcg cttcacgaga
      781 tagctgatcg gcgcgttctg gaacgccgcc gagccgtaca cgggctcgac gccccaggcg
      841 ccgcgcagca cgcccgaggc gatgttcgtg tgcgtcgtgc ccgccgtgtc ggtgtgcggg
      901 tgcgtgccgg cggcgtccgg cgcgccgctg atgctccccg ccggcgtgcc gactaccgag
      961 cggaacttca ccacgcggtg cgggttgtgg cagtcggtgc actcggcgtg gcggttggag
     1021 agattggcga cgccgagctt ggtgcgctgc tcgacgaagt cggcgccgca cttgttggtc
     1081 ggcgaggtgc agtcgacgaa gctcgcgtcg ccgaagttgc cgccgatgtc gtgcacctcg
     1141 gcgcccgcgg cctgctcggt cgaggcgatc ggcatgtggc gcggcagcgc gaagtcgccc
     1201 tggatgttcg gcaccgtcgt gcccggcgtg atcgccgtcg tcgcggccga cgtgtggcac
     1261 tggtagcagg tctgctcgat cgccgaggcc ccgccctgct tcggcgtcgc cgggctgtcg
     1321 gtgccctcgc gcagcagccg ccgcgcgccc tgcaccgtgt gcgtgtcgtg gcagttgagg
     1381 caggccgcct tccacaccgg ctggctcgac gggaattcgc gcgttcccgc ggcggtcacc
     1441 gtgtacgtct ccgtggccac cagcggattc gcgtgcgccg agtacgccca ggccccgctg
     1501 cccgcgttct tgtcgtggca ggacaggcac acgatgtcgt tcgccggatc gtagacgttc
     1561 gacggcggcg cctcctggaa gcggttcgcg cgcaggaatt tctggttgcc cttcgacggg
     1621 tcggtctcgc gcaggtgcgg atcgtggcag gtcgcgcact gcacctggcc cgtgcgcccg
     1681 acgccggtcg gctcgagcgg cagcagcggc ttgtagcccg ggctcgggat gccgaccgcg
     1741 gtgcccgacc cgggcggcca cttctgcgtc gcgtcgaccg gccgcagctc gccgtcgcgc
     1801 gcggcgagag cgctggtgaa gctcaccgac accgggtggt cgttcgtgag atcgacgccg
     1861 aggttgcgcg tcgcgcccga ggtcgtgccc gcgcccgtcg gcatcgtgcc gcccgcgccg
     1921 acgccggtca tcggcaccga gacgctgccc tgcgtcgaca ccgatccctg cccggccagc
     1981 acgttgacgt tgccgatcgc gagcgtgccg tcgtggcacg acaggcacag cttcgagctt
     2041 ccgcccggct ggtcgagcga gccctggatc gcgttcgcgt cgagcgagga cgaggtgtag
     2101 acgctgtagg tgccgcccga caccgcgcgg ttccacagcg gcgtcagcgc cggcgtcgcg
     2161 ccgtgcggcg tgtggcagaa cacgcacagc tgcgactcgg aggtcgcctt caccgtcccg
     2221 ggccccgccg cggacaggtt gtgcttggta ttgcggatgt cggcgacagg cgcggcgagc
     2281 gccgcgccca ccgacagcgc ggacagggcg agcagcgcga ggcctctagc gctcactgtc
     2341 cacctctccg ccgaggaact ggaacacggc gacgcggccg ttgaacatgt cggcgacgaa
     2401 cacgcggttt ttcgcgtcga cccacacgcc cgccggcaga tagaagcggc ccgcgtcctg
     2461 cccgacgccg ccgatcgcca tgaggaaagc gccgttgcgg tcgaacacga gcaggtggtc
     2521 gtggtaggac tcgaccacgt agacgttgcc ctcgctgtcg accgcgacgc ccttgggccg
     2581 caccaggttg ccgacgtaca gcccgcgcgc gccgaacgtg cgcaccagcc cggtgccgtc
     2641 ggtgcggaag acctcgatgc ggctgttgag cgtgtcggtg acgtagagct caccgcgcgc
     2701 gaacgcgagg tgcgtcggat agttgaactc gccctcgccc gagccgcggc gcccgagcgt
     2761 cgcgaggagc cggccgtccg cgtcgaacac cttgacgtcg tgcgcgtagg tgtcggcgac
     2821 gtagatgcgc ccgccgatcg gatcgtaggc gagcccggtc gggcgcttca gcacgccgcg
     2881 cccgaaaggc gggagcgtcg cgccgcgcgc gtcgagtcgc gcgacgatgc cgagctcggc
     2941 gtcggcgacg agcagctcgc ccgcgggccc cagcgccacc ccgaccggcg cgacgaagcc
     3001 ggtgaggccg tcggcacggt cccagacggc gagctcgccg gcgcgcgcgt cgaagacgaa
     3061 caccgcctgg cggctggcgt cggtcacgta gacgcgcccc gcgtcgtcga ccacgcccgc
     3121 ctgcggccgc tgcagcacga ccggcgcgcg ctcgcccgcg acgatgcccg cgatcgcggc
     3181 gagcacacgc tgcaggcccg gcgccggctc gcttgcgcgg aagttctgct cgccgacgag
     3241 ctgtcccgcg tagagatagc gcggggtctc gggcgcagcc ggccagatcg ggcgctgcgc
     3301 ctcgggcacg tcctcgacgc cgtagtagag cgcgcgcccg cccgggcccg cgcacgcggc
     3361 gagcgcggcc gcgaggagga tggcgaaagc gcgcgcggtc acggctacca ccgccccggg
     3421 ccgagcgtcc ggtcgccgcc gtagatcccg ctcggcaacc cggggcgtcc cgagtggcaa
     3481 aggtcgcagc ggtccgccgc ccaggcgatc tgtccgttgt ggcacatgcc gcagaagcgc
     3541 ccgtcgttga tatcggccat ctgcacgtcg ttcgcgccgg cgcgcctctt gaagccgagc
     3601 gcggcgtggc acaccttgca gcggaagcgg atgcggtgga accagtgcgg aaacacgacc
     3661 ggccgcatgc cttcccggtc cgagcgcttg ttcagcacga cgtcgccgta ctcggcctgc
     3721 gccggtccga tctcgacgag cgccgcgagc gacgccgcgg ccgcgatcgc gagaaggatg
     3781 cgcgcgatcc gcatgggtca cggcttcgcc ggctgcgacg ccgcggtccc gcgcggaacg
     3841 ctgtggcagc gcgtgcattc ggtcagcggg aaggagacgg cgccgtggca cacgccgcac
     3901 tgctcgccct gcaggatctg cagcatcgag agcttgttcg cgccggcctt cgacttgaac
     3961 aggtgctcgt ggcagttcga gcagtcgagc cagagcgtgt gctgccggtg cgggaaacgc
     4021 acgatgggcg tgccgccgcg caggttcatc agcacgtcga gatcgaggac gttcaccttg
     4081 gtctcgggca ggatgttcgt gcgcgggctg atctgcccct tgtcgagcgc ctcgacccag
     4141 cgcacctggt tgcccgccgt gtcgggcgcg agcccggaga gcgcctcgcg cggctcctgc
     4201 agcagctgca gcgacgggct cgagggatcg tgcacgccgt cgcgcgcgag cggcagccag
     4261 tcgcccgcgc gcgtcgcctg cgccgcgagc agcagcgccg ccagcaccca tcgagccgtc
     4321 ctgcgcttgc gcatgctctc tcctcacccc gggatgtaga cgccggcgcc gcggatcgcg
     4381 cgcaccagct cctgcgtcga ctcctcgagg agccggatcg cgccgcgcca gtcgcgcgcg
     4441 agcgcggc
//
LOCUS       DATDMW010000198        10625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_187834, whole genome shotgun sequence.
ACCESSION   DATDMW010000198 DATDMW010000000
VERSION     DATDMW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10625
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187834"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2111)
                     /gene="alaS"
                     /locus_tag="VI319_08570"
     CDS             complement(<1..2111)
                     /gene="alaS"
                     /locus_tag="VI319_08570"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY6863940.1"
                     /translation="MKSSEIRERFLKFFEERGHAILASSPLVPANDPTLLFTNSGMVQ
                     FKDVFLGKESRPYRRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIRFAWDLVTRVYKIPADKLWTTVYHEDDEAYDLWTKEIGVPKERVVRIGDKPGAG
                     KYQSDNFWQMADTGPCGPCSEIFYDHGPGIPGGPPGSPDADGDRYIEIWNLVFMQFDR
                     DESGKLNPLPKPSVDTGMGLERIAAVLQGVHSNYEIDLFQELIRAAARAVYGEMRGTK
                     DLKSNSLKVIADHIRACSFLVVDGVIPGNEGRGYVLRRIIRRAIRHGYKLGQKQPFFH
                     KLVPDLARVMGDAYPELRKSQQRVVQVLEAEEERFAETLENGMKVLEAALASKPKTLD
                     GETVFQLYDTFGFPMDLTADIARERGVAVDHAGFEAAMERQRERARAAGKFRMEAGVE
                     YSGGATEFHGYETLSLAAKVVALYREGARVDELRPGEPAVVVLDRTPFYAESGGQVGD
                     RGELVSAGATFSVDDTQKIQATVSGHHGALTRGTLRVGDAVDARVDALARARTMRNHS
                     ATHLMHKALREVLGPHVQQKGSLVNAEKTRFDFSHDAPLSDDQVRRVEAIVNAEVLRN
                     EPTSARVMKFDDAVKAGAMALFGEKYGDEVRVLDIGSSRELCGGTHVARTGDIGFFKI
                     VGESGVAAGVRRVEAVTGDVAV"
     gene            complement(2098..2787)
                     /locus_tag="VI319_08575"
     CDS             complement(2098..2787)
                     /locus_tag="VI319_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863941.1"
                     /translation="MTAATLHELIEGHLRSAQSSWSIGALGAIAEFERAESEPAEVRA
                     LSVVTARGAISLDLPAGCRVFARERPSAPPHLWLCLPAPEARISARAGIADLGRDSGA
                     PRAQDRDALLFDLGLGLANCEFCVRTGDASLAAALRAAEGTALLGNAPLLERLKRASP
                     HRVVRSRAGRIEVYQDIAPDGGRSPSGPHTHLLPKLLAQGRTHAAGVAIPEGWLPCLT
                     LYPLKSVHEVL"
     gene            2903..3886
                     /locus_tag="VI319_08580"
     CDS             2903..3886
                     /locus_tag="VI319_08580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6863942.1"
                     /translation="MLRLLEPLLIAADGALGRLVLGRQGFGQLVREAGLRCLDSEDLD
                     RAWRWLSLATRLTPDDAQAQFLSGVTALGRKDVAEAGRRFQTAADLDPGSSHAHLALA
                     QISLPGPGYYDVLRRIHGHLRPRTYLEVGVEAGRSLALALPGTRAIGIDPAPQLAAPP
                     APGVTVHATTSAEFFARHDVRAELGGLPIDLAFIDGMHTFESALRDFIEIEKHCAPQS
                     TILFHDGYPLDRATAERERRTKFWSGDIWRLVLALRRHRPDLSVHTVTTPPTGLGLVR
                     RLDPGSRVLEERFREIEREFLALDYSVLDADKRGMLAAVANDWEQIAPLLS"
     gene            complement(3905..5146)
                     /locus_tag="VI319_08585"
     CDS             complement(3905..5146)
                     /locus_tag="VI319_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEY6863943.1"
                     /translation="MQALQSLLGFFALHGVAWALGENRRAVAWRPVIGGMGLTLALGV
                     VLLKIPAIRQIFFGLNHALDALERATREGTAFVFGYLGGAPLPFAETQAGSTFILAFR
                     ALPLVVVVSALSALLFHWRVLPWLVRGFSLLLQKTMGVGGAIGLSSAANVFVGMVEAP
                     LVVKPYIADMSRGELFVLMSCGMATIAGTVMALYASILSPVVPDALGHILIASIISTP
                     AAIAVSALMVPVDRQTGGALALEDPASSSMDAVTRGTVDGMTLLINIVAMLLVLVALV
                     ALANQIVGLLPDVAGAPLTLQRVLGWLMAPLAWVMGVPWSEATTAGALMGTKTVLNEL
                     IAYIDLAKLPEGALEPRSRLIMTYALCGFANFGSLGIMIGGLATMAPGRRAEIVQLGG
                     RTIVSGTLATCIAGAVVGVLY"
     gene            complement(5146..6174)
                     /locus_tag="VI319_08590"
     CDS             complement(5146..6174)
                     /locus_tag="VI319_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY6863944.1"
                     /translation="MPEADNGWIDEMRSAYLYRVLAEVERGTPRAQMFDGLAKEAREQ
                     ASIWAKSAKLAAVPEFKPDLRTRVVAALARRYGPRAIRGILAAMKVRGLSVYSHAAPG
                     HPATTSVDEVGKRHRGVAGQGNLRAAVFGVNDGLVSNVSLILGVAGASPSPGVIVLSG
                     VAGLLAGAFSMAAGEYVSVRSQREMYEHQIGLERAELAEYPKAEAEELALIYAARGMP
                     QAEAHALATRTIADPRQALDTLAREELGLNPDELGSPWGAALASFLSFAIGAAVPLVP
                     FLAAGGERALAVSIALTAVSLFAVGALISLFTGRSALRDGLRMLAIGAGAGAITYAIG
                     RAMGVGLG"
     gene            complement(6198..6644)
                     /gene="recX"
                     /locus_tag="VI319_08595"
     CDS             complement(6198..6644)
                     /gene="recX"
                     /locus_tag="VI319_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEY6863945.1"
                     /translation="MARQPTLKERAIRLLAGREHTRAVLARKLAGHAGPEDDLSALLD
                     ELTQRGQLSESRYAEARARQLARKFGAARIAHELRAKGLPKEAAEAAAAAARETELDR
                     AREVWSRKFGTPPESREEKARQIRFMQSRGFSFDTIRTVTGGTEDD"
     gene            complement(6667..7719)
                     /gene="recA"
                     /locus_tag="VI319_08600"
     CDS             complement(6667..7719)
                     /gene="recA"
                     /locus_tag="VI319_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY6863946.1"
                     /translation="MVNEGKVMEEAKAKALQAALAQIEKQFGKGSIMKMDSAAAQDVQ
                     VVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLSVIAQIQKMGGTAAFIDAE
                     NALDPQYAKKLGVKIEDLLISQPDTGEQALEIADMLVRSGSIDVIVVDSVAALTPKAE
                     IEGEMGEPQMGLQARLMSQALRKLTANIKRTNTLVIFINQIRMKIGVMFGNPETTTGG
                     NALKFYASCRLDIRRIGSIKKGDEVIGSETRVKVVKNKVAPPFRDTVFDILYGEGVSR
                     EGEILELGVNCGIVDKSGAWYQYKKDRIGQGKDNARDYLKENPELADEIEAKIREKLG
                     VKNPMAASAPAVTAEV"
     gene            complement(7860..8954)
                     /gene="amrS"
                     /locus_tag="VI319_08605"
     CDS             complement(7860..8954)
                     /gene="amrS"
                     /locus_tag="VI319_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY6863947.1"
                     /translation="MSARPDPHFPGRWWHLLADGRLKCDLCPRDCKLHEGQRGACFVR
                     MRENDAMVLTTYGRSSGFCVDPVEKKPLNQFYPGSAILSFGTAGCNLACKFCQNWDIS
                     KSQDFDRLADAADPETIAKAALQLGCRSVAFTYNDPVIFAEYAMDIADACREKGVKAV
                     AVTAGYMHPEPRREFYAKMDAANVDLKAFTDEFYFKTCGARLAPVLDTLAYLRHETGV
                     WLEITTLLIPGKNDSDAEIDAECRWIARELGPDVPLHFTAFHPDWKMTDLPATPAATL
                     TRARRIALGHGLRYVYTGNVHDPEGGSTYCPGCGTAVIGRDWYKILDYGVTEDGRCKD
                     CGHAIAGRYERFTKSFGSRRIPVRLASAST"
     gene            complement(8951..10312)
                     /gene="amrB"
                     /locus_tag="VI319_08610"
     CDS             complement(8951..10312)
                     /gene="amrB"
                     /locus_tag="VI319_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY6863948.1"
                     /translation="MRPAAVAGMFYPADPRILERDIDAMLEAAGERALAPGFPKLVIV
                     PHAGYVYSGPVAANAYAALAPARGLVRRIVLLGPCHRVPVRGLALPAARAFQTPLGRV
                     PVDADAVAALRGLAQVTESAATHAQEHALEVQLPFLQRVLGEFSLVPLVVGDAAADEV
                     AEVIERLWGGPETVIVISSDLSHYLPYDVARRVDGATLKSILGFQTGIAHDQACGATP
                     IAGALVAARRRGLAPELLDYRNSGDTAGDRRQVVGYASFAFADKAAYGRAHGEQLLAI
                     ARASIASAFRRDAAVPKFDAPWLQEWRSSFVTLKLDGELRGCVGALEPHRPLAEDVAA
                     NASAAAFQDPRFAPLREPELERTELEVSLLSAPTRIAFDSHEQLLARLRPGVDGLIVQ
                     LLDDPSRRATFLPQVWESLPEREAFVAHLKRKAGLAETEPSARCVFKRYTVQKWREGD
                     PRP"
     gene            complement(10396..>10625)
                     /locus_tag="VI319_08615"
     CDS             complement(10396..>10625)
                     /locus_tag="VI319_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY6863949.1"
                     /translation="GALERSRAGLAVAVTGIAGPAGGTPAKPVGTVCFAWASRGGRAR
                     SVVRRFDGDRESVRRQSVIAALEGVLDSLEG"
BASE COUNT         1549 a         3753 c         3738 g         1585 t
ORIGIN      
        1 acggcaacgt cgccggtgac ggcttcgacg cggcgcacgc cggcggcgac gcccgattcg
       61 ccgacgatct tgaagaagcc gatgtcgccc gtgcgcgcga cgtgcgtccc gccgcacagc
      121 tcgcgcgagg agccgatgtc gagcacgcgc acctcgtcgc cgtacttctc gccgaagagc
      181 gccatcgcgc cggccttcac cgcgtcgtcg aacttcatga cgcgcgcgct cgtgggttcg
      241 ttccgcagga cctcggcgtt cacgatcgcc tcgacgcggc ggacctggtc gtccgagagc
      301 ggcgcgtcgt gcgagaagtc gaagcgcgtc ttctcggcgt tgacgagcga gcccttctgc
      361 tgcacgtgcg gccccagcac ctcgcgcagc gccttgtgca tgaggtgcgt cgccgagtgg
      421 ttgcgcatgg tgcgggcgcg cgcgagggcg tcgacccggg cgtcgacggc gtcgccgacg
      481 cggagcgttc cgcgcgtcag cgcgccgtga tggcccgaga ccgtcgcctg gatcttctgc
      541 gtgtcgtcca ccgagaacgt ggcacccgcg gagacgagct cgccgcggtc gccgacctgg
      601 ccgcccgatt cggcgtagaa aggcgtgcgg tcgagcacga ccaccgcggg ctcgcccggt
      661 cgcagctcgt cgacccgcgc gccctcgcgg tagagcgcga ccaccttcgc cgctagcgac
      721 agcgtctcgt agccgtgaaa ctcggtggcg ccgcccgagt actcgacgcc cgcctccatc
      781 ctgaacttcc ccgcggcccg cgcgcgctcg cgctggcgct ccatcgcggc ttcgaagccc
      841 gcgtggtcga ccgcgacgcc gcgctcgcgc gcgatgtcgg cggtgagatc catcgggaag
      901 ccgaaggtgt cgtacagctg gaacacggtc tcgccgtcga gcgtcttcgg cttcgacgcg
      961 agcgcggcct cgagcacctt catgccgttc tcgagcgtct cggcgaagcg ctcttcttcg
     1021 gcctcgagca cctggacgac gcgctgttgc gacttgcgca gctccgggta ggcgtcgccc
     1081 atgacgcgcg cgagatccgg gaccagcttg tggaagaacg gctgcttctg ccccagcttg
     1141 tagccgtgcc ggatcgcgcg ccggatgatc cgccgcagca cgtagccgcg gccttcgttc
     1201 ccggggatga cgccgtcgac gacgaggaag gagcaggcgc ggatgtggtc ggcgatgacc
     1261 ttgagcgagt tgctcttcag gtctttcgtg cccctcattt cgccataaac tgcgcgcgcc
     1321 gccgcgcgga tgagctcctg gaacagatcg atctcgtagt tcgagtgcac gccctgcagc
     1381 accgcggcga tgcgctcgag gcccattccc gtgtcgaccg acggcttcgg cagcgggttg
     1441 agcttccccg actcgtcgcg gtcgaactgc atgaacacga ggttccagat ctcgatgtag
     1501 cggtcgccgt ccgcatcggg agagccgggc ggcccaccgg ggattccggg accgtgatcg
     1561 tagaagatct cgctgcacgg gccgcacgga ccggtgtccg ccatctgcca gaagttgtcg
     1621 ctctggtact tgccggcgcc cggcttgtcg ccgatgcgaa cgacgcgctc tttcggcacg
     1681 ccgatctcct tggtccagag atcgtaggcc tcgtcgtcct cgtggtagac ggtggtccag
     1741 agcttgtcgg ccgggatctt gtagacccgg gtcaccaggt cccaggcgaa ccggatcgca
     1801 tcgcgcttga agtagtcgcc gaaggagaag ttgcccagca tctcgaagaa cgtgtggtgg
     1861 cgcgccgtgt agccgacgtt ctcgaggtcg ttgtgcttgc cgcccgcgcg cacgcagcgc
     1921 tgcgccgtcg tcgcgcgcct gtacggccgc gactccttgc cgaggaacac gtccttgaac
     1981 tgcaccatcc ccgagttcgt gaagagcagc gtcgggtcgt tggcgggcac gagcgggctc
     2041 gaggcgagga tcgcgtggcc gcgctcctcg aagaacttca ggaacctctc gcggatttca
     2101 gaggacttca tggacggatt ttagcggata cagggtgagg cacggcagcc aaccctccgg
     2161 gatcgcgacg cccgcggcat gcgtgcggcc ctgcgcgagc agcttgggca ggaggtgcgt
     2221 gtgcggcccg ctcggggagc gtccgccgtc cggcgcgatg tcctggtaca cctcgatacg
     2281 ccctgcgcgc gagcggacga cccgatgcgg gctcgcgcgc ttgagccgct cgagcagcgg
     2341 cgcgttcccg agcagcgccg tgccttcggc cgcgcgcagc gcggcggcga gagacgcgtc
     2401 cccggtgcgc acgcagaact cgcagttggc gagacccagg ccgagatcga acagaagcgc
     2461 gtcccggtcc tgcgcgcgcg gcgcacccga gtcgcggccg agatcggcga tgcccgcacg
     2521 cgcgctgatg cgcgcctcgg gcgcgggcag gcacagccac agatgcggcg gggccgacgg
     2581 ccgctcgcgg gcgaacacgc ggcagccggc ggggagatcg agcgatatcg cgccgcgcgc
     2641 ggtcaccacc gagagcgcac ggacctcggc gggctccgac tccgctcgct cgaactcggc
     2701 gatcgcgccg agcgcgccga tgctccagct cgactgcgcg gagcgcaggt gtccttcgat
     2761 cagctcgtgc agcgtggcgg cggtcatcgg attttcccgg tcggtgcgcg tgaattgtgc
     2821 gacgcgccca cccgggcgcg caatgactgc ggcggacgcc tgcgctatgc tcacgactcg
     2881 tcatgaggtc gacgcacgat cgatgctgcg ccttctcgaa cctctgctga tcgcagcgga
     2941 cggtgcgctc ggccgtctcg tgctgggccg ccaaggcttc gggcagctcg tgcgcgaagc
     3001 aggcctgcgg tgcttggact cggaggacct cgaccgggcg tggcgctggc tgtcgctcgc
     3061 gacgcgcctg acgcccgacg acgcgcaagc gcagtttctc tccggggtga cggcactcgg
     3121 tcgcaaggac gtcgccgaag cgggcaggcg cttccagacc gccgcggacc tcgacccggg
     3181 cagcagccac gcccacctgg cgctcgcgca gatcagcctt cccgggccgg gctactacga
     3241 cgtgctgcgc cggatccacg ggcatctgcg gccgcgcacc tacctcgagg tcggcgtgga
     3301 ggccgggcgc tcgctcgcgc tggccctccc cgggacgcgc gccatcggca tcgatccggc
     3361 gccgcagctc gcggcgccgc cggcccctgg cgtcactgtg cacgcgacca cgagcgcgga
     3421 gttcttcgcc cgccacgacg tccgcgccga gctcggcggc ctgccgatcg atctcgcgtt
     3481 catcgacggc atgcacacat tcgagtcggc gttgcgcgat ttcatcgaga tcgagaagca
     3541 ctgcgcgccg cagtcgacga tcctcttcca cgacggctat ccgctggacc gcgccaccgc
     3601 ggagcgcgag cgccggacga agttctggag cggcgacatc tggcgcctgg tgctcgcgct
     3661 tcgccggcac cggccggatc tcagcgtgca tacggtgacg acgcccccga cgggtctcgg
     3721 cctcgtgcgc cgcctcgacc cgggctcgcg agtgctcgag gagcgcttcc gagagatcga
     3781 gcgcgagttc ctcgcgctcg actattcggt gctggacgcg gacaagcggg gcatgctggc
     3841 cgccgtggcg aacgactggg agcagatcgc tccgctcctg tcgtagcgcg cccgcggcgc
     3901 gcggtcagta gagcacgccc acgaccgccc cggcgatgca ggtggcgagc gtgcccgaga
     3961 cgatggtgcg tccgccgagc tgcacgatct ccgcgcggcg cccgggcgcc atggtggcga
     4021 gcccgccgat catgatgccg aggctgccga agttcgcgaa gccgcacagc gcgtaggtca
     4081 tgatcagccg gctgcggggc tcgagcgcgc cttccgggag cttcgcgaga tcgatgtacg
     4141 cgatcagctc gttcagcacg gtcttcgtgc ccatgagcgc acccgcggtg gtcgcctcgg
     4201 accagggcac gcccatgacc cacgcgagcg gcgccatcag ccagccgagc acgcgctgca
     4261 gcgtgagcgg cgcgccggcg acgtcgggaa gcagcccgac gatctggttg gcgagggcga
     4321 cgagcgcgac caggacgagc agcatcgcga cgatgttgat cagtagcgtc atgccgtcga
     4381 cggtgccccg ggtcaccgcg tccatgctgc tcgaggccgg gtcctcgaga gcgagcgcgc
     4441 cgccggtctg gcggtcgacc ggcaccatca gcgccgagac cgcgatcgcg gccggcgtgc
     4501 tgatgatcga tgcgatcagg atgtggccga gcgcgtcggg gacgaccggc gagaggatcg
     4561 acgcgtacaa ggccatcacg gtgcccgcga tcgtcgccat gccgcacgac atgaggacga
     4621 agagctcgcc gcggctcatg tcggcgatgt agggcttcac cacgagcggc gcctcgacca
     4681 taccgacgaa cacgttcgcc gcggacgaga ggccgatcgc cccgcccacg cccatcgtct
     4741 tctgcaggag cagcgagaag ccgcgcacga gccagggcag cacccgccag tggaagagca
     4801 gcgccgagag cgccgagacc acgaccacca gcggcagcgc ccggaacgcg aggatgaagg
     4861 tcgacccggc ttgcgtctcg gcgaacggca gcggcgcgcc gccgaggtag ccgaaaacga
     4921 acgcggtgcc ctcgcgcgtg gcgcgctcga gcgcgtcgag cgcatggttc aggccgaaga
     4981 agatctgcct gatcgccggg atcttgagca atacgacgcc gagcgccagc gtcagcccca
     5041 tgccgccgat gaccgggcgc caggcgacgg cgcgacggtt ctcaccgagc gcccaggcga
     5101 cgccgtgcag cgcgaagaac ccgagcaggc tttggagcgc ctgcatcagc cgagacccac
     5161 gcccatcgcg cgtccgatcg cgtacgtgat cgcgccggcg cccgcgccga tcgcgagcat
     5221 gcgcaagccg tcgcgcaagg cgctgcggcc cgtgaacagg ctgatcagcg cgccgaccgc
     5281 gaacagcgac accgcggtga gcgcgatcga cacggcgagc gcgcgctcgc cgccggcggc
     5341 gaggaacggc acgagcggca ccgcggcgcc gatcgcgaac gacaggaacg acgcgagcgc
     5401 cgcgccccag ggcgagccga gctcgtcggg gttgagccca agctcctcgc gcgcgagcgt
     5461 atcgagcgcc tgccggggat cggcgatggt gcgcgttgcc agcgcgtgcg cctcggcctg
     5521 cggcatgccg cgcgccgcgt agatcagcgc cagctcctcg gcctcggctt tggggtactc
     5581 ggcgagctct gcgcgctcca ggccgatctg gtgttcgtac atctcccgct gcgagcgcac
     5641 cgagacgtac tcgcccgcgg ccatcgagaa ggcgccggcg agcagccccg cgaccccgga
     5701 gagcacgatg acgcccgggc tcggcgacgc gcccgcgacg ccgaggatca ggctcacgtt
     5761 cgagaccagg ccgtcgttga cgccgaacac cgcggcgcgc aggttgccct gccccgcgac
     5821 cccgcgatgg cgcttgccga cctcgtcgac cgaggtcgtc gccgggtgtc ccggcgcggc
     5881 gtggctgtag accgagagcc cgcgcacttt catcgcagcg aggatgccgc ggatcgcccg
     5941 cgggccgtag cgccgcgcga gcgcggcgac gacccgggtg cgcaggtcgg gtttgaattc
     6001 gggaacggcg gcgagcttcg cgctcttcgc ccagatcgac gcctgctccc gggcttcctt
     6061 cgcgagaccg tcgaacatct gcgcgcgagg tgtcccgcgc tccacttccg cgagcacgcg
     6121 gtagagatac gccgagcgca tctcgtcgat ccagccgttg tccgcctcag gcataccggg
     6181 gcggattgtg gcagaactca gtcgtcctcg gttccgcccg tcacggtccg tatggtgtcg
     6241 aaggagaagc ctcgcgattg catgaagcga atctgccgcg ccttctcctc gcgcgactcg
     6301 gggggggtcc cgaacttccg gctccagact tcgcgggcgc gatcgagctc ggtctcgcgc
     6361 gccgcagccg cggcggcctc cgcagcctcc ttgggcaggc ccttggcgcg aagctcgtgc
     6421 gcgatgcgcg ccgcgccgaa cttgcgcgcg agctggcgcg cgcgggcctc ggcatagcgc
     6481 gactcggaaa gctgcccgcg ctgcgtcagc tcgtcgagca gcgcggagag gtcgtcttcg
     6541 gggccggcgt ggccggcgag cttgcgggcg agcaccgcgc gcgtgtgctc gcggccggcg
     6601 aggagtcgga tggcgcgctc cttcagggtg ggttgacgcg ccatccgccc tgccgttcgg
     6661 gtaaaactag acttcggccg tcaccgccgg cgcggatgcc gccatcggat tcttcacccc
     6721 gagcttctcg cggatcttcg cctcgatctc gtcggcgagc tcggggttct ccttgagata
     6781 gtcgcgggcg ttgtccttgc cctggccgat gcggtccttc ttgtactggt accaggcgcc
     6841 cgacttgtcc acgatgccgc agttgacgcc gagctcgagg atctcgccct cgcgcgacac
     6901 gccctcgccg taaaggatgt cgaagaccgt gtcgcggaag ggcggcgcga ccttgttctt
     6961 caccaccttg acgcgggtct cggagccgat cacctcgtcg cccttcttga tcgagccgat
     7021 gcggcggatg tccagccggc acgaggcgta gaacttgagc gcgttgccgc cggtcgtcgt
     7081 ctcgggattg ccgaacatga cgccgatctt catgcggatc tggttgatga agatgacgag
     7141 cgtattggtg cgcttgatgt tggcggtgag ctttcgcagc gcctggctca tcagccgcgc
     7201 ctgcaggccc atctgcggct cgcccatctc gccctcgatc tcggccttgg gcgtgagcgc
     7261 cgcgaccgag tcgacgacga tgacgtcgat cgagcccgag cgcaccagca tgtcggcgat
     7321 ctcgagcgcc tgctcgccgg tgtcgggctg cgagatgagc aggtcctcga tcttcacgcc
     7381 gagcttcttc gcgtactggg ggtcgagcgc gttctccgca tcgatgaagg cggccgtgcc
     7441 gcccatcttc tggatctggg cgatgaccga gagggtcagc gtcgtcttgc ccgaggattc
     7501 cggcccgtaa atctcgacca cccgcccgcg cggcagcccg ccgacgccga gcgcgatgtc
     7561 gaggccgagc gacccggtcg aaaccacctg cacgtcctgc gccgcggccg agtccatctt
     7621 catgatcgag cccttgccga actgcttctc gatctgcgcg agcgccgcct gcagcgcctt
     7681 ggctttcgct tcttccatga ccttcccttc gttgaccatt ggattatctc ccaattgtgc
     7741 gttgaagcgg gcgcccgccg ccggacaggc tcgctcgaag ggatgaaacg gtcggagcga
     7801 cgagccggtt gactgtatct ttatacagtc cggccgaaaa agcaagcccc ggctccgggt
     7861 caggtggacg cggaagcgag ccggacggga atccggcggg aaccgaagga ttttgtgaac
     7921 cgctcgtagc ggcccgcgat cgcgtggccg cagtccttgc agcggccgtc ctcggtgacc
     7981 ccgtaatcga ggatcttgta ccaatcgcgc ccgatcaccg cggtcccgca gcccgggcag
     8041 taggtgctgc cgccttccgg gtcgtgaacg ttgcccgtat acacatagcg cagcccgtgg
     8101 ccgagcgcga tgcggcgcgc gcgggtgagc gtcgccgcgg gcgtcgccgg caggtcggtc
     8161 atcttccagt cggggtggaa ggcggtgaaa tgcagcggca cgtccggccc gagctcgcga
     8221 gcgatccagc ggcattccgc gtcgatctcg gcgtcggagt cgttctttcc cgggatcagc
     8281 agcgtcgtga tctcgagcca gacgccggtc tcgtgcctca ggtacgcgag cgtgtcgagc
     8341 accggcgcca ggcgcgcccc gcacgtcttg aagtagaact cgtcggtgaa cgccttgagg
     8401 tcgacgttcg ccgcgtccat tttggcgtag aactcgcggc gcggctccgg gtgcatatac
     8461 ccggccgtca ccgccaccgc cttcacgccc ttctcgcggc aggcatcggc gatgtccatc
     8521 gcgtactcgg cgaagatgac cgggtcgttg taggtgaacg cgaccgagcg gcagccgagc
     8581 tggagcgcgg ccttcgcgat cgtttcggga tcggccgcgt cggcgagccg gtcgaagtcc
     8641 tgcgacttcg agatgtccca gttctggcag aacttgcacg cgagattgca gcccgcggtg
     8701 ccgaacgaga ggatggccga gcccgggtag aactggttga gcggcttctt ctcgaccgga
     8761 tcgacgcaga agcccgacga gcggccgtag gtcgtcagca ccatcgcgtc gttctcgcgc
     8821 atgcgcacga agcaggcgcc gcgctgccct tcgtgcagct tgcagtcgcg cggacacagg
     8881 tcgcacttca ggcggccgtc ggccagcagg tgccaccagc gcccgggaaa atgcgggtcg
     8941 ggacgcgcgc tcacggccgc gggtcgcctt cgcgccattt ctggacggtg tagcgcttga
     9001 agacgcagcg cgccgacggc tcggtctcgg cgaggccggc cttgcgcttc aggtgcgcga
     9061 cgaaggcctc gcgctcggga agcgattccc acacctgcgg caggaaggtc gcccgccggg
     9121 acgggtcgtc gagaagctgc acgatcagcc cgtcgacccc gggacgcagc cgcgcgagca
     9181 gctgctcgtg cgagtcgaac gcgatccgcg tcggcgcgga cagcagcgac acctcgagct
     9241 cggtgcgctc gagctcgggc tcgcgcagcg gcgcgaagcg cgggtcctgg aacgccgcgg
     9301 cgctcgcgtt ggcggcgacg tcctcggcga gcgggcggtg cggctcgagc gctccgacgc
     9361 agccgcgcag ctcgccgtcg agcttgagcg tgacgaagct cgagcgccat tcctggagcc
     9421 acggcgcgtc gaatttcggg acggccgcgt cgcgccggaa cgcgctcgcg atcgacgcgc
     9481 gcgcgatcgc gagcaactgc tcgccgtgcg cgcgcccata ggcggctttg tccgcgaacg
     9541 cgaacgacgc gtagccgacc acctggcgcc ggtcgccggc cgtgtccccc gagttgcggt
     9601 agtcgagcag ctcgggcgcg aggccgcggc gcctcgccgc gaccagggcg ccggcgatgg
     9661 gcgtcgcgcc gcacgcctgg tcgtgcgcga tcccggtctg gaagccgagg atgctcttca
     9721 gcgtcgcgcc gtccacgcgg cgcgccacgt cgtagggaag gtaatgcgag aggtccgagc
     9781 tgatcacgat gacggtctcc gggccgcccc agagccgctc gatcacctcg gcgacctcgt
     9841 cggccgccgc gtcgccgacg acgagcggaa ccagcgagaa ctcgcccagc acccgctgca
     9901 ggaacggcag ctgcacctcg agcgcgtgct cctgcgcgtg cgtggcggcg gactcggtca
     9961 cctgcgctag cccgcgcagc gcggctacag catcggcgtc gaccggtacc cgcccgagcg
    10021 gcgtctggaa ggcgcgcgcc gcgggcagcg ccaggccgcg caccgggacg cggtggcaag
    10081 gcccgagcag cacgatgcgc cgcaccagcc cgcgcgcggg cgcgagcgcg gcgtacgcgt
    10141 tcgcggccac cgggccgctg tagacgtacc ccgcgtgcgg aacgatgacc agcttcggga
    10201 accccggcgc gagcgctcgc tcaccggccg cctcgagcat ggcgtcgatg tcgcgctcga
    10261 ggatccgggg atccgccgga tagaacatgc ccgccacggc ggcggggcgc atgccggcgg
    10321 ctcggatgtc gagaaagccc atcccctcaa tataggggag cggcgcggcc ggtccaagac
    10381 cgggccacga ttttttcagc cctcgagcga gtcgagcacg ccttcgagcg ccgcgatcac
    10441 cgactggcgg cgcacgctct cgcggtcgcc gtcgaagcga cggaccacgc tgcgcgcccg
    10501 cccgccgcgg ctcgcccatg cgaaacatac ggtgccgacc ggcttcgcgg gcgtaccgcc
    10561 cgcgggtccc gcgatcccgg tcaccgcgac cgcgagcccg gcgcggctgc gctcgagcgc
    10621 gcccg
//
LOCUS       DATDMW010000199         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1806553, whole genome shotgun sequence.
ACCESSION   DATDMW010000199 DATDMW010000000
VERSION     DATDMW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806553"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VI319_08620"
     CDS             complement(<1..200)
                     /locus_tag="VI319_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863950.1"
                     /translation="MLLHFLGEHRDEILAKARARAITRKAPAADPAEIEKGIPLFFDQ
                     LVSILEGRTPAKELKSDAVFHGG"
     gene            complement(209..1063)
                     /locus_tag="VI319_08625"
     CDS             complement(209..1063)
                     /locus_tag="VI319_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY6863951.1"
                     /translation="MAAPRPGWRGFAGSLKRDWTRNGLTDAAAALTFYAVLALFPFIL
                     FTVAAASLILRIDQVQALGAALARDTPAALAPILLARFRELASRPRVGMLTFSAIAAI
                     WSATAGVKSLVTALNSACGVPETRRAWRVYAMAFAFMLGSSVLVLLAAATALAAPLAA
                     SKLGASSGALAVWLRIGAASLMMIVWAALFYVLPDVRQTVRSIIPGSIAGVLVWLAAT
                     LVFSFYVSHFGSFGVVYGALGGIVILLIWMWLSSLALLLGAEINALLRRRADWRGGAA
                     LTHRGRSP"
     gene            1508..>2475
                     /locus_tag="VI319_08630"
     CDS             1508..>2475
                     /locus_tag="VI319_08630"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY6863952.1"
                     /translation="MNPTTPQVRFTGRGAFHAELQQRAQDFFEGSGRSRHGGWRIGAK
                     IAVMLSWLAASWALAVFAPLPAWTLALLAVSIGFAMAGVGFCAMHDANHGATSSSARV
                     NRIVSLSLDLVGASSVLWREKHNVRHHTYTNIAGADPDLEGGSPFLRLAPWQRRRAWH
                     RFQHLYVWLIYAVFPLKWWFFDDLRELSRLRPRDRLSLLAGKGVFLAWAVVIPAMLHP
                     GWGLVALWAIAVATLGNVLAAVFQLAHCMDDAEFVDATAERGDWAEHQLSTTVDFAPR
                     NALLGWYLGGLNFQVEHHLFARVCHVHYPALAAMVRDASARHGLRYR"
BASE COUNT          361 a          875 c          895 g          344 t
ORIGIN      
        1 ccgccgtgga agacggcgtc gctcttcagc tccttcgcgg gagtgcggcc ctcgaggatc
       61 gagacgagct gatcgaagaa cagcgggatg cccttctcga tctcggccgg atccgccgcc
      121 ggcgcctttc tcgtgatggc gcgcgcgcgg gccttcgcga ggatttcgtc gcggtgctcg
      181 ccgagaaagt ggaggagcat caaagaccct atggactgcg tccccgatgt gtcaacgcgg
      241 cgccgcctcg ccagtcggcc cggcgacgaa ggagcgcgtt gatctccgcg ccgaggagca
      301 aggcgagcga ggagagccac atccagatga ggaggatgac gatccccccc agggcgccat
      361 agacgacgcc gaagctcccg aagtgcgaga cgtagaacga gaagacgagc gtcgccgcga
      421 gccagacgag cactccggcg atcgacccgg ggatgatcga tcgcacggtc tgcctgacgt
      481 ccgggaggac gtagaagagc gcagcccaga cgatcatcat cagcgacgcg gcgccgatcc
      541 gcagccagac ggcgagggct cccgaggagg ccccgagctt gctcgcggcg agcggcgccg
      601 cgagcgcggt cgcggccgcg agaaggacca ggaccgacga gccgagcatg aaggcgaagg
      661 ccatcgcgta gaccctccac gcgcgccggg tctcgggcac gccgcaggcc gagttcagcg
      721 cggtcacgag gctcttgacg ccggccgtcg ccgaccagat cgcggcgatc gcgctgaacg
      781 tcagcatgcc caccctcggt cgcgaggcga gctcccggaa tcgggcgagc aggatcggcg
      841 cgagcgctgc cggcgtgtcg cgcgcgagag ccgctccgag cgcctgcacc tgatcgatcc
      901 ggaggatcag gctcgccgcc gcgacggtga agaggatgaa cgggaagagg gcgaggacgg
      961 cgtagaacgt gagcgcggcg gcggcgtcgg tcaggccgtt gcgcgtccag tcgcgcttga
     1021 gcgagcccgc gaagccgcgc caccccgggc gcggcgctgc catcatgcga gacgggacca
     1081 tgccctcgaa cgtaggtcgg ggcgaggcgg cccgctgcca gcgcgtcggc gcgccgcggc
     1141 ccgcgtgact atcctcccgg cacgcccgcg ccgaccgtcg tcgacctccg gacggggagg
     1201 cgaccgcaat gaagcacgtc ggggacgtcc tgggccggtt tcgcgggtgg gtcgagcggc
     1261 tgagcgatcg gcgcgaccgg aagctgttcg agccgcctgc gccgttcgcg cccgaccgcc
     1321 cgcgccgttc cgactggcac tccacggcga atgaccggat gacggacgac ggcgctcctc
     1381 cgctccgcgc tggtggcgcg ccgccggcgc gtcgtggagg agactgaccg gcggctactt
     1441 ccgccgccgg ctgccgtcga agcgcgcgcg atgacccccc ggtgactcgc cgacactact
     1501 tggcaggatg aaccccacca cgccgcaggt tcgcttcacc ggccgcggcg cgttccacgc
     1561 cgagctgcag cagcgcgcgc aggacttctt cgaggggagc ggccgctcgc ggcacggcgg
     1621 ctggcgcatc ggcgcgaaga tcgccgtgat gctctcctgg ctcgcggcgt cgtgggcgct
     1681 ggcggtgttc gcgccgctgc cggcgtggac gctcgcgctg ctggccgtct ccatcgggtt
     1741 cgccatggcg ggcgtgggct tctgcgccat gcacgacgcg aatcacggcg cgacctcgtc
     1801 gagcgcgcgg gtcaaccgga tcgtctcgct ctcgctcgac ctggtcggcg cgagctcggt
     1861 gctctggcgc gagaagcaca acgtccggca ccacacgtac acgaacatcg ccggcgccga
     1921 tcccgacctc gagggaggat cgcccttcct ccgcctcgcg ccgtggcaac gccggcgcgc
     1981 ctggcatcgc ttccagcatc tctatgtctg gctcatctac gcggtgtttc cgctcaagtg
     2041 gtggttcttc gacgatctgc gcgagctgtc gcgcctgcgc ccccgcgacc ggctctcgct
     2101 gctcgccggc aagggggtct tcctcgcctg ggcggtggtg attccggcga tgctgcaccc
     2161 ggggtggggc ctggtcgcgc tctgggcgat cgcggtggcg acgctcggca acgtgctcgc
     2221 cgccgtcttc cagctcgcgc actgcatgga cgacgccgag ttcgtcgatg ccacggcgga
     2281 gcggggcgac tgggccgaac accagctctc gacgacggtg gacttcgcgc cgcgaaacgc
     2341 gctcctcggc tggtacctgg gcgggctcaa cttccaggtc gagcaccatc tcttcgcgcg
     2401 cgtgtgccac gtccactatc cggcgctggc ggcgatggtg cgcgacgcca gcgcgcggca
     2461 cggcctgcgc taccg
//
LOCUS       DATDMW010000200         4137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_684344, whole genome shotgun sequence.
ACCESSION   DATDMW010000200 DATDMW010000000
VERSION     DATDMW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684344"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..705
                     /locus_tag="VI319_08635"
     CDS             112..705
                     /locus_tag="VI319_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863953.1"
                     /translation="MALAKPSAKPQARRPVTTAADLLAHFMPRGVLRDLQQLANAFQN
                     EDLFRDYVGQRIGYLAVIVLIFVLIATVCAATTMLSVPRLAPPPAGAGLKFLAFLCGL
                     FVWVAGTMAQIYLFFGWVQERALEKAGKLPPHKFRFDRIGFEFLLKKRRIVVPCTLIL
                     ICLLVPLAILAVRAPLVALTLFIVGLGVPALYTRLDP"
     gene            complement(702..3272)
                     /gene="glgP"
                     /locus_tag="VI319_08640"
     CDS             complement(702..3272)
                     /gene="glgP"
                     /locus_tag="VI319_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY6863954.1"
                     /translation="MTQGTQGTSYSLEVHPKLPQRLARLEELAGNLWYSWDRATRALF
                     ARLDPDLWEATGHSPKALLKRIDQQRLLDAATDPIFLSAFNRVLSAFDTYHSERFSKP
                     GTAALQPGDQVAYFCAEFGFHESFPMYSGGLGILAGDHCKAASDHQLPFIAIGLLYRQ
                     GYFVQTVDAEGSQRAEYVDSDFENLPVAPVRKSDGSPLTIELPLPGRSLQVKVWQARA
                     GHVTLYLLDTDVPENRENDRHIAHRLYGGDRTTRLEQEIILGMGGVRAIIAMGLRPSV
                     WHINEGHAAFLILERMRYLVLGGLEFETALEAVASNTVFTTHTAVPAGHDQFPEQIIR
                     PFLEAYCAELRVPVEKVASLARVASGGDFNMTALAVRGSRFQNGVSRIHGDVSAEMLR
                     DFWPKVPFRENPIRYITNGVHVPTFLAPEWGDVFDRYLGIGWLHRLDDPATLREIEAI
                     PDHEFWSVRQTLKTQLLEMLRARLRTQHFRNHGSESHLDRMLRLADPNEPNVLTVGFA
                     RRFATYKRAALLFSNLDVLRQIVGDSKRPILFIFAGKAHPADEPGQDLIRRIVRASRM
                     PEFEGKILFVEGYDLNIARRLVTGVDVWLNNPVYPLEACGTSGMKAGMNGAVNLSVLD
                     GWWDEAYAHDNGWAIKPAPSSFDPERRDREEAHTLYEILQDHVLPLYYERDHKPYSPG
                     WVRMAKRSMAALLPRFNASRMLNEYVSNFYGAASRQGWRHAEKDYEGARTLAQWKARV
                     RGAWPGVSARRADAAVRRIEFGASVPVEVRVRLNGLAPEDVAVELLMERNDRQPVEHQ
                     AYMLAPAGGEGRSGDAGEHRYALELKPEMCGKLDYRIRLYPKHPLMTHPFEMGLMVWI
                     "
     gene            complement(3311..>4137)
                     /locus_tag="VI319_08645"
     CDS             complement(3311..>4137)
                     /locus_tag="VI319_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6863955.1"
                     /translation="ESPRWLITHGRAERAEAEVAAIEAAVAAERGPLPAAHGALAMRM
                     RGHVAWSEIARLMAFRYRRRAVLGLSLMASQAFFYNAIFFTYGLVLTRFYGVADERIG
                     AYILPFALANFAGPLVLGWLFDTVGRRKMIALTYALAGAGLIGAGWAFAREALDAQGL
                     TLAWAAIFFVASAAASSAYLTVSEVFPLEIRAVSISIFYAIGTGVGALLTPALFGALI
                     GTGSREWLFGGYAAAGLLMCAAAAVAWIAGVDAERKPLEAVAAPLSSDPVPEAVAG"
BASE COUNT          613 a         1490 c         1400 g          634 t
ORIGIN      
        1 ggagcggcac aaatccacgg ggctttttcg cgccggactg catcccgtcc ggggcgcggt
       61 tatgctgtcg tcacgcgccg cccaaagcgc gcgagaacga ggagaagcgc catggccctt
      121 gccaagccgt ccgcgaaacc ccaggcccgc cgtcccgtca cgaccgccgc cgacctgctc
      181 gcgcatttca tgccgcgcgg cgtgctgcgc gacctgcagc agctggcgaa cgcgttccag
      241 aacgaggatc tgttccggga ttacgtgggg cagcgcatcg gctacctcgc ggtcatcgtg
      301 ctgatcttcg tcctgatcgc cacggtgtgc gcggcgacca cgatgctgtc ggtgccgcgg
      361 ctggcgccgc cgcccgcggg cgccggcctc aagttcctcg cgttcctctg cggcctcttc
      421 gtctgggtcg cgggcacgat ggcgcagatc tatctcttct tcggctgggt ccaggagcgc
      481 gcgctcgaga aggcggggaa gctgccgccg cacaagttcc gcttcgaccg catcggcttc
      541 gagttcctgc tgaagaagcg gcgcatcgtc gtgccgtgca cgctgatcct gatctgcctc
      601 ctggtgccgc tcgcgatcct ggcggtgcga gcgccgctcg tcgcgctcac gctcttcatc
      661 gtcgggctcg gcgtccccgc cctctacacg cgcctcgacc cctagatcca caccatcagg
      721 cccatctcga agggatgggt catgagcggg tgcttgggat agagccggat gcggtagtcg
      781 agcttgccgc acatctcggg cttcagctcg agcgcatagc gatgctcgcc cgcatctcca
      841 cttcggccct cgccgcccgc cggcgcgagc atgtacgcct ggtgctcgac cggctgccgg
      901 tcgttgcgct ccatcagcag ctcgaccgcc acgtcctccg gcgcgagccc gttcagccgc
      961 acgcgcacct cgaccggcac gctcgcgccg aactcgatgc gccgcaccgc cgcatcggcc
     1021 cgccgcgccg acacgcccgg ccacgcgccg cgcacgcgcg ccttccattg cgcgagcgtg
     1081 cgcgcgccct cgtaatcctt ctccgcgtgc cgccagccct ggcgcgacgc cgctccgtag
     1141 aaattcgaca cgtactcgtt cagcatccgt gacgcgttga accgcggcag cagcgcggcc
     1201 atcgagcgct tcgccatgcg cacccagccg ggcgagtacg gcttgtggtc gcgctcgtag
     1261 tagaggggca gcacgtgatc ctgcaggatc tcgtacagcg tgtgcgcctc ctcacggtcg
     1321 cggcgctcgg ggtcgaacga ggacggcgcc ggcttgatcg cccagccgtt gtcgtgcgcg
     1381 tacgcctcgt cccaccagcc gtcgagcacc gagaggttca ccgcgccgtt catcccggcc
     1441 ttcatgcccg acgtgccgca ggcctcgagc ggatacaccg ggttgttcag ccacacgtcg
     1501 acgccggtga ccaggcgccg cgcgatgttg aggtcgtagc cctcgacgaa caggatcttc
     1561 ccttcgaact cgggcatgcg cgacgcgcgc acgatgcgcc ggatcaggtc ctgccccggc
     1621 tcgtcggccg ggtgggcctt gcccgcgaag atgaacagga taggccgctt cgagtcgccg
     1681 acgatctggc gcagcacgtc gaggttggag aacagcagcg ccgcgcgctt gtacgtcgcg
     1741 aagcgccgcg cgaagccgac ggtgagcacg ttgggctcgt tgggatcggc gaggcgcagc
     1801 atgcggtcga ggtgagactc gctgccgtga ttgcggaagt gctgcgtgcg cagcctcgcg
     1861 cgcagcatct cgagcagctg cgtcttcagc gtctggcgca cgctccagaa ctcgtggtcc
     1921 gggatcgcct cgatctcgcg cagcgtggcc ggatcgtcga gccggtgcag ccagccgatg
     1981 ccgagatagc ggtcgaacac gtcgccccac tccggcgcga ggaacgtcgg cacgtgcacg
     2041 ccgttcgtga tgtagcggat cgggttttcc ctgaacggca ccttcggcca gaagtcgcgc
     2101 agcatctccg ccgagacgtc gccgtgaatg cgcgacacgc cgttctggaa gcgcgagccg
     2161 cgcacggcga gcgccgtcat gttgaagtcc ccgccgctgg cgacgcgcgc gagcgacgcg
     2221 accttctcca ccgggacgcg cagctccgcg cagtaggcct cgaggaacgg gcggatgatc
     2281 tgctccggga actggtcgtg gcccgccggc accgccgtgt gcgtcgtgaa cacggtgttg
     2341 gacgcgaccg cctcgagcgc ggtctcgaac tccaggcccc cgagcacgag gtagcgcatg
     2401 cgctcgagga tcaggaacgc cgcgtggccc tcgttgatgt gccacacgct cggccgcagg
     2461 cccatcgcga tgatggcgcg cacgccgccc atgccgagga tgatctcctg ctccaggcgc
     2521 gtcgtgcggt cgccgccgta gagacggtgc gcgatgtggc ggtcgttctc gcggttctcc
     2581 ggcacgtcgg tgtcgagcag gtagagcgtc acgtggcccg cgcgcgcctg ccacaccttc
     2641 acctggagcg atcgccccgg cagcggcagc tcgatcgtga gcggcgagcc gtcgctcttc
     2701 ctcaccggcg cgaccggcag gttctcgaaa tccgagtcga cgtactcggc gcgctggctg
     2761 ccctccgcgt cgacggtctg cacgaagtag ccctggcggt agagcagccc gatcgcgatg
     2821 aacggcagct ggtgatcgct cgccgccttg cagtggtcgc ccgcgaggat gccgaggccg
     2881 cccgagtaca tcgggaagct ctcgtggaag ccgaactcgg cgcagaagta cgcgacctgg
     2941 tctcccggct gcagcgccgc cgtgcccggc ttggagaagc gctccgagtg gtaggtgtcg
     3001 aacgccgaca gcacgcggtt gaacgcggac aggaagatcg ggtccgtagc cgcgtcgagc
     3061 agccgctgct ggtcgatgcg cttcagcagc gccttgggcg agtgcccggt cgcctcccac
     3121 agatcgggat cgagacgcgc gaacagcgcg cgcgtcgcgc ggtcccagct gtaccagaga
     3181 ttgcccgcca gctcctcgag gcgcgcgagg cgctgcggca gcttcggatg gacttccagc
     3241 gaatacgacg ttccctgcgt tccctgcgtc atctgcgttc tcgttccttt tcaattcgtc
     3301 ttttcgggat tcatcccgcg accgcttccg gaaccggatc cgagctgagc ggcgccgcca
     3361 ccgcctcgag cggcttgcgc tcggcgtcga cgcccgcgat ccacgcgacc gccgcggccg
     3421 cgcacatcag cagaccggcg gccgcgtagc cgccgaacag ccattcgcgg ctgcccgtcc
     3481 cgatcagcgc gccgaagagc gccggcgtca gcagcgcccc gaccccggtc ccgatcgcgt
     3541 agaagatcga gatcgagacg gcgcggatct cgagcggaaa gacctcgctc accgtcaggt
     3601 aggccgagct cgccgccgcc gacgcgacga agaagatcgc cgcccacgcg agcgtgagtc
     3661 cctgcgcgtc gagcgcctcg cgcgcgaagg cccagcccgc gccgatcagc cccgctcccg
     3721 cgagcgcgta ggtgagcgcg atcatcttgc gccggcccac ggtatcgaac agccacccga
     3781 ggacgagcgg gccggcgaag ttcgccaggg cgaacggcag gatgtaggcg ccgatgcgct
     3841 cgtcggcgac gccgtagaag cgcgtcagca ccagcccgta ggtgaagaag atcgcgttgt
     3901 agaagaacgc ctgcgacgcc atgagcgaaa gcccgagcac cgcgcggcgg cggtagcgga
     3961 aagccatgag gcgtgcgatc tcggaccagg cgacgtgccc gcgcatgcgc atcgcgagcg
     4021 ccccgtgcgc cgcgggcagc gggccgcgct cggccgcgac cgccgcctcg atggccgcga
     4081 cctcggcctc ggcgcgctcg gcgcgcccgt gggtgatcag ccagcgcggg ctctcgg
//
LOCUS       DATDMW010000201         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2430231, whole genome shotgun sequence.
ACCESSION   DATDMW010000201 DATDMW010000000
VERSION     DATDMW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430231"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /gene="serC"
                     /locus_tag="VI319_08650"
     CDS             <1..710
                     /gene="serC"
                     /locus_tag="VI319_08650"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY6863956.1"
                     /translation="NFTFAPEQSGWQLDPKAAYVHYTSNETIGGVEFHWIPDTGHVPL
                     VADASSHILSRPLDVSKFGLIYAGAQKNIGPAGLVIVIVREDLLGRASPNTPTVLDYK
                     VQAEADSMSNTPASYSWYIAGLVFQWLKRQGGLAAMEKKNIEKSKLLYDYLDSSGFFK
                     SPVRKQDRSRMNIPFTLADAKLDAEFLKGAEARGMFQLKGHRSVGGMRASIYNAMPIE
                     GVRRLVEYMREFEAKHG"
     gene            737..1921
                     /locus_tag="VI319_08655"
     CDS             737..1921
                     /locus_tag="VI319_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY6863957.1"
                     /translation="MAAKKYEILVLNQISPAGLKRLPGERYKCGKEVAKPDAILVRSA
                     DMHAMEIGDTVRAIGRAGAGTNNIPVKKMSERGVPVFNAPGANANAVKELVLAGMLMA
                     ARNLVPAVNFVSALDCDDKTLGERVEAEKKQFAGMELPHHTLGVIGLGKIGSLVADAA
                     IKLGMNVLGYDPQITVEAAWSLPSQVKRANSIEEVLKTCQFVTVHVPLLPATKHLINA
                     KNLDLAHAGAILLNFSREEIVDAKAVAKALESKKLRTYVTDFPAEVLCGNPAVIALPH
                     LGASTREAEDNSAVMVVDEVREYLENGNIQNAVNFPDVVMGRESPYRVAIANANVPNM
                     VGQISSAMANEQLNIHNMMNRSRGEMAYTLVDVDSPVKGSLIEQLCRIKGVLAVRYLP
                     AV"
     gene            1938..>2125
                     /gene="pheA"
                     /locus_tag="VI319_08660"
     CDS             1938..>2125
                     /gene="pheA"
                     /locus_tag="VI319_08660"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY6863958.1"
                     /translation="MADDIAKHRAAIDALDDRLLELLNERAAHAQAIGKLKGNGKAYR
                     PEREAEVQRRARAANRGPL"
BASE COUNT          401 a          705 c          719 g          300 t
ORIGIN      
        1 agaacttcac cttcgcgccc gagcagtcgg gctggcagct cgaccccaag gcggcgtacg
       61 tgcactacac ctcgaacgag accatcggcg gcgtcgagtt ccactggatc ccggacacgg
      121 gccatgtgcc gctcgtcgcc gacgcctcgt cgcacatcct gtcgcgcccg ctcgacgtct
      181 cgaagttcgg cctgatctat gccggcgcgc agaagaacat cgggcccgcg ggcctggtga
      241 tcgtgatcgt gcgcgaggac ctgctgggac gcgcgtcgcc gaacacgccg accgtgctcg
      301 actacaaggt gcaggcggag gccgactcga tgtcgaacac gcccgcgtcc tactcgtggt
      361 acatcgccgg cctcgtgttc cagtggctga agcgccaggg cgggctggcc gcgatggaga
      421 agaagaacat cgagaaatcg aagctgctgt acgactatct cgactcgagc ggcttcttca
      481 agagcccggt ccggaagcag gaccgctcgc gcatgaacat cccgttcacg ctcgccgacg
      541 ccaagctcga cgccgagttc ctgaagggcg ccgaagcgcg cggcatgttc cagctcaaag
      601 gccaccgctc ggtcggcggc atgcgcgcct ccatctacaa cgcgatgccg atcgagggcg
      661 tgcggcgcct ggtcgagtac atgcgcgagt tcgaggcgaa gcacggctga gcgcgcgctt
      721 ggtttatagt ctcgggatgg ccgcgaaaaa gtacgaaatc ctggtgctga accagatctc
      781 gccggcgggc ctcaagcgcc tccccggcga gcggtacaag tgcggcaagg aagtcgccaa
      841 gcccgacgcg atcctggtgc gctcggccga catgcacgcg atggagatcg gcgacaccgt
      901 gcgcgccatc ggccgcgcgg gtgcgggcac gaacaacatc ccggtgaaga agatgagcga
      961 gcgcggcgtg ccggtgttca acgcgccggg cgcgaacgcg aacgcggtga aggagctggt
     1021 gctcgccgga atgctgatgg cggcgcgtaa cctggtgccc gcggtgaatt tcgtgagcgc
     1081 gctcgactgc gacgacaaga cgctcggcga gcgcgtcgag gccgagaaga agcagttcgc
     1141 gggcatggag ctgccgcacc acacgctcgg cgtgatcggc ctgggcaaga tcggcagcct
     1201 cgtcgccgac gcggcgatca agctcgggat gaacgtgctc ggctacgatc cgcagatcac
     1261 ggtcgaggcg gcgtggagcc tgccgtcgca ggtgaagcgc gccaactcga tcgaggaagt
     1321 gctcaagacg tgccagttcg tcacggtcca cgtgccgctg ctgccggcga cgaagcacct
     1381 gatcaacgcc aagaacctcg acctggcgca cgcgggggcg atcctgctca acttttcgcg
     1441 cgaggagatc gtcgacgcca aggcggtcgc caaggcgctc gagtcgaaga agctcaggac
     1501 ctacgtcacc gatttcccgg cggaagtcct ctgcggcaac ccggcggtca tcgcgctgcc
     1561 gcacctcggc gcctcgacgc gcgaggccga ggacaacagc gccgtcatgg tcgtcgacga
     1621 ggtgcgcgag tacctcgaga acggcaacat ccagaacgcc gtgaactttc ccgacgtcgt
     1681 gatggggcgc gagtcgcctt accgcgtcgc gatcgccaac gccaacgtgc ccaacatggt
     1741 cgggcagatc tcgagcgcga tggcgaacga gcagctcaac atccacaaca tgatgaaccg
     1801 gtccagggga gagatggcct acacgctggt cgacgtcgac agcccggtga agggctcgct
     1861 gatcgagcag ctgtgccgca tcaagggcgt cctcgcggtc cgctacctgc ccgcggtcta
     1921 gggctatcat cgagcccatg gccgacgaca tcgccaagca ccgggccgcg atcgacgccc
     1981 tcgacgacag gctgctcgag ctgctgaacg agcgcgccgc gcacgcgcag gcgatcggca
     2041 agctgaaggg caacggcaag gcctaccgtc ccgagcgcga ggccgaggtg cagcggcgcg
     2101 cgcgcgccgc gaaccgcggc ccgct
//
LOCUS       DATDMW010000202         6573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1556562, whole genome shotgun sequence.
ACCESSION   DATDMW010000202 DATDMW010000000
VERSION     DATDMW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556562"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..442)
                     /locus_tag="VI319_08665"
     CDS             complement(113..442)
                     /locus_tag="VI319_08665"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY6863959.1"
                     /translation="MADSWLGWTLIAGTAALVYLTRAVFVLPGSRLRLPPLAERVLRY
                     APAAALAAIIAPDLALVHGALAVSPDNPRLVGGIVAFAVAAATRSVTLTIIVGMLAMT
                     AVRLIAA"
     gene            complement(444..1136)
                     /locus_tag="VI319_08670"
     CDS             complement(444..1136)
                     /locus_tag="VI319_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY6863960.1"
                     /translation="MSAFRDGAASAVSLLFGMVPWGLVAGLAMVSAGLTPAQAVVMGL
                     LVYAGSAQLAVLPLLIAKAPLWVMYATALAVNLRYVIYSAVLAPYFERLSRPWRVLLS
                     YVMVDAMFALFVGRFRPEDRAPHRHWFFLGGSVVLWIGWQAASWLGIFGGALIPRAWS
                     LEFAATLSLIALLMPLLYDRAVVLGALAAGGVALAGAGLPFNLGLICAIAAGIAAGLA
                     ASRVQRARARGA"
     gene            1247..2251
                     /locus_tag="VI319_08675"
     CDS             1247..2251
                     /locus_tag="VI319_08675"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEY6863961.1"
                     /translation="MRSRGILRGLAFFLVLAAALLAGPGARADIVVMQNGDRITGKVK
                     HKAGDTLDVDTDYAGTVELRWSQVKSIQTDAPVEIVVEGEPASSLFWFSGDESDVRAD
                     RIVYINPSPEEWGHAFAYKTRLNVSGTWTSGNSRSGRGQADGELTARSSDVRYILSGK
                     ARRESVSGEETASNWLGSLKRDHFLLDPAHFIYARASLERDRFKDISLRSTLGGGFGW
                     QLLDSEITKLTFGAGLDAVRTLRITAPDEHYPALGWDVRLSHWLGAKTAELVHEEEGF
                     WNLHDTRQVTLRSRTGVRVPVAKGMVANASLNVDWEREPAAGRKPVDATLLLGVGAEW
                     "
     gene            2348..3097
                     /locus_tag="VI319_08680"
     CDS             2348..3097
                     /locus_tag="VI319_08680"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY6863962.1"
                     /translation="MIFAFPALGASAQSADDYALLGAGVRTRPAYDGSASQKTDVIPA
                     VRYYGRPWFARTTQGMLEGGARMEIAPGLAFGIQLAYEPGRKRSESPFLREHDIPDLD
                     VGASLGLHLEWDGKIGPAPVNVLLRQRSNAEAIRGDQIDLRGTMGVYGHGPVSIGVFG
                     QTTWASQKSTNTLYGITPEESVTSGLPAFAAKGGVMFASLGLLGGYDFNPRWTALWSI
                     EARRLEGDARRSPLAERTSNHYASISIARRF"
     gene            complement(3103..3999)
                     /locus_tag="VI319_08685"
     CDS             complement(3103..3999)
                     /locus_tag="VI319_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6863963.1"
                     /translation="MKRSIRAAIVLAAALAAPSYAQDDFSKVEIQTQKLADTVYMMTG
                     SGGNLGLSAGEDAVFLIDDQFAPLTPKIEAAIAKITDKPVKFVLDTHWHFDHTGGNEN
                     LGKTGAVIFAHENVRKRLSTEGFIEFLGMKTSPSPKAALPLITFTRDLTFHINGDEVF
                     VLYAPNAHTDGDAIIHFKKSNVIHTGDTFFNRMYPFIDTSSGGTLDGMIRAADLVLRL
                     ADDDTKIIPGHGPLGNKADLQAYRDMLADVAARMRRLVRQGKTLDQAVAAKPTAKFDE
                     QWGKGFLPPEKFVAMLYGNLKR"
     gene            complement(4044..5345)
                     /gene="rmuC"
                     /locus_tag="VI319_08690"
     CDS             complement(4044..5345)
                     /gene="rmuC"
                     /locus_tag="VI319_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY6863964.1"
                     /translation="MDSVLFGLTFLVAGVAIGGVAVWLVIREKLKTAEDRARAETAVE
                     LATLRERLEAQKDKLAFVSEAREDLGARFKNLANEILEEKSRRFDETNRQGLETLLKP
                     LREKIGEFQAKVEQTYDKESRERLTLQSEIKRLAELNVQISEDADNLARALKGDSKTQ
                     GTWGELVLEKVLESSGLRLNEEYVVQKAFLNEDDRRSRPDVVVNLPEKRHVVIDSKVS
                     LTAYDRYVAAEDESERQVALRQHALSVRSHIMELSAKNYQNLYGLESLDFVLMFVPVE
                     SACMLAMQSERDLFLDALRKNVLIVSPSSLIGVLRTIAHIWRQEYQNRNAKELARHCA
                     ALYDKFVGFVEDLEDIGRKLEATRLSYEGAKSKLSSGRGNLIRQAERIRSLGVKPAKA
                     LPAHLVEEAVDNAESDLDQQMSLDEEPAANVVRLPEERKNP"
     gene            complement(5356..5745)
                     /locus_tag="VI319_08695"
     CDS             complement(5356..5745)
                     /locus_tag="VI319_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY6863965.1"
                     /translation="MSAPPAPYERVGGDAIVRALVSRFYDLMDSDSQYAGIRGLHPAD
                     LAPAREKLYLFLSGWLGGPQLYAAKYGHPMLRARHLHAAIGIAERDQWLACMSRAMAD
                     VEIEEGLRRELYNAFYKTADWMRNREG"
     gene            complement(5742..>6573)
                     /locus_tag="VI319_08700"
     CDS             complement(5742..>6573)
                     /locus_tag="VI319_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6863966.1"
                     /translation="DYADERARRQVEREFNVTDMTDLPPDNTVSEGRWFTKQDFDGGA
                     VSVEKWIAERLGIGLGDRLEFEAGGRSFSAPVTSIRKLEWDTMRTNFFFVTTPKLLRD
                     APTTYMTGFHSGEAELTARLSRAFPNLTVIDVGSIFRQVRAMMDQVIRAVQFVFLFAL
                     VAGMLVLYAALLATQDERAQESALMRALGASRAQILGAQRAEFTVLGLLAGVLASLGA
                     TAIGWVLAERVFQFDYVWNAWIWAVGPGLGLACILFNAWAGARAALSRPPIAALREAA
                     "
BASE COUNT         1025 a         2257 c         2284 g         1007 t
ORIGIN      
        1 cgtacgcgcg ccgcgaaggc gtcggccgtc cgctcgcgca tgggcagcat cgcgcacgcg
       61 gccgccagcg cggcgacgac gcacgacgcg aggagcggac gcgcccgcat ggctacgccg
      121 cgatcagccg caccgcggtc atcgccagca tcccgacgat gatggtgagc gtgacgctgc
      181 gcgtcgccgc cgcgaccgcg aacgcgacga ttccgccgac gaggcgcgga ttgtcgggcg
      241 acaccgcgag cgcgccgtgg accagcgcga gatcgggcgc gatgatcgcg gcgagcgcgg
      301 ccgcgggcgc gtagcgcagc acgcgctcgg caagcggggg cagtcgcaac cggctgccgg
      361 gaagcacgaa cacggcgcgc gtaaggtata cgagcgccgc cgttccggcg atcagggtcc
      421 acccgagcca ggaatcggcc acgtcaggct ccccgcgcgc gggcgcgctg cacgcgtgag
      481 gccgccagcc ccgccgcgat gccggcggcg atcgcgcaga tcaacccgag gttgaagggc
      541 agcccggccc cggcgagggc gacgcctccg gcggcgagcg cgccgagcac gaccgcgcgg
      601 tcgtagagca gcggcatgag cagcgcgatg agcgacagcg tcgcggcgaa ctcgagcgac
      661 catgcgcgcg ggatgagcgc cccgccgaag atgcccagcc aggacgccgc ctgccagccg
      721 atccacagga ccacggagcc gccgaggaag aaccagtgcc ggtgcggggc ccggtcttcc
      781 gggcggaagc ggccgacgaa gagcgcgaac atcgcatcga ccatgacgta gctgagcagc
      841 acgcgccacg gccgcgacag gcgctcgaag taaggcgcga gaacggcgct gtagatgacg
      901 taacggaggt ttaccgcgag cgcggtcgcg tacatcaccc agagcggcgc tttcgcgatg
      961 agaagcggca gcacggcgag ctgcgccgag cccgcgtaca cgagcagccc catcacgacg
     1021 gcctgcgcag gcgtgagccc ggcgctcacc atcgcgaggc ccgcgaccag gccccagggc
     1081 accatgccga agagcagcga gacggcgctc gccgcaccgt cgcgaaaagc cgacattcga
     1141 tcgccgtgca tttcccgcga ttccatcaaa tggacgatgc cggccggcgg caaatgacac
     1201 aatccgcggc cagcattcgc attgtcccat ctcccctgga gccggtttga ggtcgcgcgg
     1261 catccttcgc gggctggcgt tcttcctcgt cctcgcggcc gcgctgctcg ccggtccggg
     1321 cgcgcgcgcc gacatcgtcg tcatgcagaa cggcgaccgg atcaccggca aggtgaagca
     1381 caaggccggc gacacgctcg acgtcgatac cgattatgcc ggcacggtcg agctgcgatg
     1441 gagccaggtc aagtcgatcc agaccgatgc cccggtcgag atcgtggtcg agggcgagcc
     1501 ggcgtcgagc ctgttctggt tctcgggcga cgagtccgac gtgcgggcgg accgcatcgt
     1561 ctacatcaat ccgtcgcccg aggaatgggg acacgcgttc gcgtacaaga cgcggctcaa
     1621 tgtctccggg acctggacct cgggcaacag ccgcagcggc cgcggccagg ccgacggcga
     1681 gctcaccgcg cgctcgagcg acgtgcgcta catcttgagc ggaaaggcgc ggcgcgagtc
     1741 ggtctcgggc gaggagaccg cgtcgaactg gctcgggtcg ctcaagcgcg accattttct
     1801 cctcgacccg gcgcacttca tctacgcccg ggcgtcgctc gagcgcgacc ggttcaagga
     1861 catcagcctg cgctcgacgc tcggcggcgg cttcggctgg cagctgctcg acagcgagat
     1921 cacgaagctc acgttcggcg ccggcctcga cgcggtgagg acgctgcgca tcaccgcgcc
     1981 cgacgagcac tatcccgcgc tcggctggga cgtgcgcctg tcgcactggc tcggcgcgaa
     2041 gacggcggag ctcgtgcacg aggaggaagg cttctggaac cttcacgaca cgcggcaggt
     2101 gacgctgcgc agccgcaccg gcgtgcgcgt gccggtggcg aagggcatgg tcgcgaacgc
     2161 ctcgctcaac gtcgactggg agcgcgagcc cgcggcgggg cgcaagccgg tcgacgcgac
     2221 gctgctcctc ggcgtcggcg cggaatggtg aggccgcggc ttgggaaaac cgcgccggtc
     2281 ggttatcctt gcggcctttg ccgggagcga cattcgaatg cgaagttccg cgagatgggc
     2341 agccgccatg atcttcgcgt ttcccgcgct cggtgcgagc gcgcagagcg ccgacgacta
     2401 cgcgctgctc ggcgccgggg tgcggacgcg cccggcgtac gacggctcgg cgtcgcagaa
     2461 gaccgatgtg atccctgcgg tgcgctatta cggccgtccg tggttcgcgc gcacgaccca
     2521 ggggatgctc gagggcggcg cgcgcatgga gatcgcgccc ggcctcgcgt tcggcatcca
     2581 gctcgcctac gagccgggac gcaagcgcag cgagtcgccg ttcctgcgcg agcacgacat
     2641 ccccgatctc gacgtcggcg cgtcgctcgg gttgcacctg gaatgggacg gcaagatcgg
     2701 cccggcgccg gtgaacgtgc tcctgcggca gcgctcgaac gccgaggcga tccgcggcga
     2761 ccagatcgac ctgcgcggca cgatgggcgt ctacggccac ggtcccgtga gcatcggcgt
     2821 gttcgggcag accacctggg ccagccagaa atcgaccaac acgctctacg gcatcacgcc
     2881 cgaggaatcc gtgacgagcg ggctcccggc gttcgcggcg aaaggcggcg tgatgttcgc
     2941 gagcctgggc ttgctcggcg gctacgactt caatccgcga tggacggcgc tgtggagcat
     3001 cgaggcccga aggctcgaag gcgacgcccg acgcagcccg ctcgcggagc gcacctccaa
     3061 ccactacgcg agcatcagca tcgcgcgccg gttctagagg cgctagcgct tcaggttgcc
     3121 gtagagcatc gccacgaact tctcgggcgg caggaagccc ttcccccact gctcgtcgaa
     3181 cttggccgtc ggcttcgccg cgacggcctg gtcgagcgtc ttgccctggc gcacgagccg
     3241 gcgcatgcgc gcagcgacgt ccgcgagcat gtcgcggtag gcctggagat cggccttgtt
     3301 cccgagcgga ccgtggcctg ggatgatctt cgtgtcgtcg tcggcgagcc tgagcaccag
     3361 gtcggccgcg cggatcatgc cgtcgagcgt cccgccgctc gacgtgtcga tgaacgggta
     3421 catcctgttg aagaaggtgt cgcccgtgtg gatgacgttg ctcttcttga agtggatgat
     3481 ggcgtcgccg tcggtatgcg cgttcggcgc atagagcacg aacacctcgt cgccgttgat
     3541 gtggaaggtc aggtcgcggg tgaacgtgat cagcggcagc gcggccttcg gcgacgggct
     3601 ggtcttcatg ccgaggaact cgatgaagcc ctcggtggag aggcgcttgc gcacgttctc
     3661 gtgggcgaag atcaccgccc cggtcttgcc gaggttctcg ttgcccccgg tgtgatcgaa
     3721 gtgccagtgc gtgtccagca cgaacttcac cggcttgtcg gtgatcttcg cgatcgcggc
     3781 ctcgatcttg ggcgtgagcg gcgcgaactg gtcgtcgatc aggaacaccg cgtcctcgcc
     3841 ggccgagagc ccgaggttgc cgcccgagcc ggtcatcatg tagaccgtgt cggcgagctt
     3901 ctgcgtctgg atctcgacct tggagaaatc gtcctgggcg taggacggcg ccgcgagcgc
     3961 ggcggcgaga acgattgcag cgcggatgct tcgcttcatc acggctctcc cctggcgtaa
     4021 atgccggcgg cgccggctcg gtgctacggg ttcttgcgct cctccgggag ccgcacgacg
     4081 ttcgccgccg gttcttcgtc gaggctcatc tgctggtcga gatcgctctc ggcgttgtcg
     4141 acggcctcct ccacgaggtg cgcgggcagg gccttcgcgg gcttcacgcc gaggctgcgg
     4201 atgcgctcgg cctgccggat gagatttccg cggcccgacg agagcttgct cttcgccccc
     4261 tcgtagctca gccgcgtggc ctccagcttg cggccgatgt cctccaggtc ctcgacgaag
     4321 ccgacgaact tgtcgtagag cgcggcgcag tgccgcgcga gctccttggc gttgcggttc
     4381 tggtattcct ggcgccagat gtgggcgatc gtgcgcagca cgccgatcag gctgctcggg
     4441 ctgacgatca ggacgttctt gcgcagcgcg tccaggaaca ggtcgcgctc ggattgcatc
     4501 gcgagcatgc acgcggattc gaccgggacg aacatcagca cgaagtcgag cgactcgagc
     4561 ccgtacaggt tctggtagtt cttcgccgag agctccatga tgtgcgagcg gaccgacagg
     4621 gcgtgctggc gcagggccac ctggcgctcc gactcgtcct cggcggcgac atagcggtcg
     4681 tacgccgtga gcgagacctt ggaatcgatg acgacgtgcc gcttctcggg gagattgacg
     4741 acgacgtcgg ggcgcgaacg ccggtcgtcc tcgttcagga aggctttctg cacgacgtat
     4801 tcttcgttca ggcgcaaccc cgagctctcg agcacttttt cgagcaccag ctcgccccag
     4861 gtcccttgcg tcttcgagtc gcccttcagc gcccgcgcga gattgtccgc gtcctcgctg
     4921 atctgcacgt tcagctcggc gaggcgcttg atctcgctct gaagcgtgag ccgctcgcgc
     4981 gactccttgt cgtacgtctg ctcgaccttc gcctggaact cgccgatctt ctcgcgcagc
     5041 ggcttgagca gcgtctcgag gccctgccgg ttcgtctcgt cgaagcgccg cgacttctct
     5101 tcgaggatct cgttggcgag attcttgaag cgggcgccga ggtcctctct cgcctcggac
     5161 acgaaggcga gcttgtcctt ttgcgcctcg aggcgctcgc gcagggtcgc gagctcgacc
     5221 gcggtctcgg cccgcgcgcg gtcttcggcg gtcttcagct tctctcgaat gaccagccag
     5281 acggcgacgc cgccgatcgc cacgccggcg acgagaaacg tcaggccgaa cagcaccgag
     5341 tccatcgaag cgccttcatc cttccctgtt ccgcatccag tccgcggtct tgtagaaggc
     5401 attgtatagc tcccggcgca ggccttcctc gatttcgacg tcggccatcg cgcgcgacat
     5461 gcacgcgagc cactggtcgc gctcggcgat cccgatcgcg gcgtgcaggt ggcgcgcccg
     5521 cagcatggga tggccgtact tcgcggcgta gagctgcggg ccgccgagcc agcccgaaag
     5581 aaagaggtag agcttctcgc gcgccggggc gaggtcggcc gggtgcagcc cgcggatgcc
     5641 ggcgtactgg gagtccgagt ccatcaggtc gtagaaccgc gagacgagcg cgcgcacgat
     5701 cgcgtcgccc ccgacccgct cgtaaggcgc cggcggcgcg ctcacgccgc ctcgcgcagc
     5761 gccgcgatcg gcggccggct gagcgcggct ctcgcgcccg cccacgcgtt gaacaggatg
     5821 cacgcgagcc cgagcccggg cccgaccgcc cagatccacg cgttccagac gtaatcgaac
     5881 tggaacacgc gctcggcgag cacccagccg atcgccgtgg cgccgagcga ggcgagcacg
     5941 cccgcgagca ggccgagcac ggtgaactcg gcgcgctgcg cgccgaggat ctgggcacgg
     6001 ctcgcgccga gcgcgcgcat cagcgccgac tcctgcgcgc gctcgtcctg cgtcgcgagc
     6061 agcgccgcgt acagcaccag catcccggcg acgagcgcga acaggaacac gaactgcacg
     6121 gcgcggatca cctggtccat catggcgcgc acctggcgga agatcgagcc gacgtcgatg
     6181 acggtgaggt tcgggaaggc gcgcgacagg cgcgcggtca gctcggcctc cccgctgtga
     6241 aatcctgtca tgtacgtcgt gggcgcgtcg cgcagcagct tcggcgtggt gacgaagaag
     6301 aagttcgtgc gcatcgtgtc ccactcgagc ttgcggatgc tcgtcaccgg tgcgctgaag
     6361 ctgcgtcctc ccgcctcgaa ctcgagccgg tcgccgagcc cgatgcccag gcgctcggcg
     6421 atccacttct cgaccgacac cgcgcctccg tcgaaatcct gcttcgtgaa ccagcgtccc
     6481 tcgctcaccg tgttgtcggg cggaagatcg gtcatgtcgg tgacgttgaa ctcgcgctcg
     6541 acctgccggc gcgcgcgctc gtcggcgtag tcc
//
LOCUS       DATDMW010000203         6767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_435994, whole genome shotgun sequence.
ACCESSION   DATDMW010000203 DATDMW010000000
VERSION     DATDMW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435994"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..989
                     /locus_tag="VI319_08705"
     CDS             84..989
                     /locus_tag="VI319_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY6863967.1"
                     /translation="MTLIDPRTIHVLKNPGAFTLQVLKAFQANQGLLLAGAVAYYALL
                     SMVPLLILIVIALSHVIDREVLLETLRQALVYVVPGKSREVVGELRSFLEHRDTVGWV
                     LLATMLFFAQLGFKVLESAISVIFVHRVRRRRRPLFVTLLLPFGYIFLIGAALFAGTY
                     MIAVLIAIGGESLVVFGHAFSLGGTSRFLIYVAGVAGEILLISAIYYFMPAGSASVRP
                     ALVGGATAGLLWEVIRYALGWYFATLSQVSVVYGSLATAIVVLLSLEIAATLLLLGAQ
                     VIAEYERIGLGAAPREPVPMRTEAA"
     gene            complement(1006..1263)
                     /locus_tag="VI319_08710"
     CDS             complement(1006..1263)
                     /locus_tag="VI319_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863968.1"
                     /translation="MQEILNYLKSRGEQLDTDIAKATGMSIENVRRNVNKLSAMGEVM
                     VCRSIRFHNGKEKEQLLCRISGYIPPPAPGRKPKAQPKIPV"
     gene            complement(1379..2479)
                     /locus_tag="VI319_08715"
     CDS             complement(1379..2479)
                     /locus_tag="VI319_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY6863969.1"
                     /translation="MLFEPFSLRAIALKNRMVMSPMTRSRAIEAATPNALMAEYYAQR
                     AGAGLIITEGTSPSPNGLGYARIPGLYNDAQAAGWRHVTDAVHAKGGKIFVQLMHTGR
                     VTHALNLPDRAEVVGPTVEICPGEMYTDRRGMQPHTPPRAMEEKDIRHAVDEYAHAAR
                     LAIQAGFDGVELHGANGYLIEQFLNANVNKRTDRWGGSIENRNRFALEVARAAANAIG
                     AEKLGIRLSPYGAFNATGAFPEVDAQYVALVKELSSLGLLYIHVLDHSAMGAPKVPDA
                     IKAKLRETFKGAYIAAGGFDKASAEQWLKEGRADLIAFGRPFLANPDLPERYRKGAAL
                     NEPDMATFYTPGEKGYTDYPRLSAEPARDAAR"
     gene            2617..3933
                     /locus_tag="VI319_08720"
     CDS             2617..3933
                     /locus_tag="VI319_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY6863970.1"
                     /translation="MRNVLLGLVALCAGAACAQSDPAPGRSPLSSAAQGVYTAARPRL
                     VQIRTVVTAAGRQSTLGSGFVVSGDGLALTNYHVVSKYALEPATYRLEYTSPDGTSGK
                     VALLAIDVANDLAVVRLDRRGGPSFQFDARALHGDLPKGERLYSMGNPLDLGFAIVEG
                     TYNGLVERSYNERMHFSGALNPGMSGGPTVTADGHVVGVNVARRFGAELVSFLVPGRF
                     AAALLERAARAEPLSPKTVRAEIGRQLASWQAGLYKAVGAAGFRDAALGPYRALESSA
                     PWFRCWASTNADRVPRPRAIVEATSCNTETQLFVADDLGTGHIGVTHAYVKSLDLNAF
                     QFATFLSQQYAPSLSGWNRKWQTRQRCHDDFVETAGPAPGPPLRAVWCARAYRDFDDL
                     YDVSVTVVTQDRGDAALVSKLSMQGVAYGEAAALARRFVEATRIAP"
     gene            3930..4892
                     /locus_tag="VI319_08725"
     CDS             3930..4892
                     /locus_tag="VI319_08725"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY6863971.1"
                     /translation="MIWVEILSRHHHDVQARHRCPGPVVRIGRGYDNDVVIDDPYVAP
                     RHLVVRRSENGELVAEDLGSANGTLVEPGAERRERVALDGTRVLRIGRTRLRIRDALQ
                     AVAPERVLRPAQRTWPATLALIAAVVLLQLLADWLGETTEPRLSRYAKPLLVLPLIVV
                     AWTSAWAVLSRIFAGQAQLGRHLLIAMCGLLAIEAYDAVPEYAAFALSWGPLAGARVA
                     GLWLIIGAVCVFHLRAISPARLRLKAGLVAALAGIPIALQALAQSDLGAGYSGERYVQ
                     LLKPPAFRFSALQDESAFFAGTARLKDRLDRARTEDSPADSDDD"
     gene            complement(4889..5569)
                     /locus_tag="VI319_08730"
     CDS             complement(4889..5569)
                     /locus_tag="VI319_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6863972.1"
                     /translation="MPGPTSKTLALALAAGLAAGCQTTDPYSGESKTTRSTYGALIGA
                     GAGAAIGMISGRDAKQRRERVLIGAGVGALSGGAIGAYQDRQENELRAQMRDSGVTVT
                     RKGDDIVLNMPGNITFRTASADLNGQFYKVLDGVALVAKKYDKTILEVAGHTDNVGGE
                     AYNRQLSQRRADSVAQYLESKGIAQARIMTAAGGEDHPIASNATEAGRSQNRRVEVTI
                     APLTQAER"
     gene            5818..6051
                     /locus_tag="VI319_08735"
     CDS             5818..6051
                     /locus_tag="VI319_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863973.1"
                     /translation="MDPSLIGAEIAWLRELRDAKQNKQPVPSLPPEVAFRLRMRGHVS
                     ANAHGEYGITIRGQSELTEREHGLAADSQRSTL"
     gene            complement(6033..>6767)
                     /locus_tag="VI319_08740"
     CDS             complement(6033..>6767)
                     /locus_tag="VI319_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6863974.1"
                     /translation="RAEAVKALLLAKAGSTRIADPTFSIDWHFTSGDSLESASIEEMA
                     NDVLHDGAYPEIHGGVRGFIARYLDAPETVLVLQGPPGTGKTRLIRAILGEMSRRKGE
                     HAQAIYTGDMKALAADEIYVKFITGEHDAFIVEDADHLLKPRADGNEHLHRFLTIADG
                     VVRAQGRKIIFSTNLPNVGDLDDALIRPGRCFARVHVRTLTGAEAKALAEEIAAGDAE
                     KLARAARVFGASEGRRSLAEVYSVLR"
BASE COUNT          866 a         2350 c         2428 g         1123 t
ORIGIN      
        1 gtgcgaggtc ttcgacggcg cgggccgcga gaagtacttg gacgatctcg cgtttcgttc
       61 ctgaacgacc gaactttgcc cccatgaccc tgatcgaccc caggaccatc cacgtcctga
      121 agaaccccgg cgccttcacg ctccaggtgc tgaaggcttt tcaggcgaac caaggcctgc
      181 tgctcgcggg cgcggtggcg tattacgcgc tgttgtcgat ggtgccgctg ctgattctga
      241 tcgtgatcgc gctgtcgcac gtgatcgacc gggaggtgct gctggagacg ttgcgccagg
      301 cgctggtgta cgtggtgccg gggaagagcc gggaggtggt gggggagctg cggtcgtttc
      361 tcgagcatcg cgacacggtc ggctgggtgc tgctcgcgac gatgctgttt ttcgcgcagc
      421 tcgggttcaa ggtgctggag agcgcgatct cggtgatttt cgtgcaccgg gtgcgcaggc
      481 gcaggcggcc gctcttcgtc acgctgctgc tgccgttcgg ctatatcttc ctgatcggcg
      541 cggcgctgtt cgcggggacg tacatgatcg cggtgctgat cgcgatcggc ggggagagcc
      601 tggtggtgtt cgggcacgcc ttttcgctcg gcggcacgtc gcgcttcctg atctacgtcg
      661 cgggcgtggc cggggagatc ctgctgatct cggcgatcta ctacttcatg ccggcgggca
      721 gcgcgtcggt gcggccggcg ctggtcggcg gggcgacggc gggcctgctg tgggaggtga
      781 tccgctatgc gctcggctgg tacttcgcga cgctgtcgca ggtgagcgtg gtgtacggct
      841 cgctcgccac cgcgatcgtg gtgctgctca gcctggagat cgccgcgacg ctgctcctgc
      901 tcggcgcgca ggtgatcgcg gagtacgagc gcatcgggct cggcgcggcg ccgcgggagc
      961 ccgtgccgat gcgcacggaa gccgcctgaa ccggcggcgc ggtcttcaga ccgggatctt
     1021 cggctgcgcc ttgggcttgc ggccgggcgc gggcggcggg atgtagccgg agatgcggca
     1081 caggagctgc tctttctcct tgccgttgtg gaagcggatg gagcggcaca ccatgacttc
     1141 gcccatggcg gagagcttgt tcacgttgcg gcggacgttc tcgatcgaca tgccggtcgc
     1201 tttcgcgatg tcggtgtcga gctgctcgcc gcggctcttc aggtaattca ggatttcctg
     1261 caagtcggat gctccttggt gatcgcgccc atcgacggag cggtataggt tacgggaaag
     1321 cgcccggcgg ctcgatgatt ctttcgccga gcgcgcaagc cgctgattcg tcggccatct
     1381 atcgcgcggc gtccctcgcc ggctcggccg agagcctcgg ataatcggtg taccccttct
     1441 cccccggcgt atagaacgtc gccatgtccg gctcgttgag cgcggcgcct ttgcggtagc
     1501 gctcgggcag gtccgggttg gcgaggaacg ggcggccgaa ggcgatcagg tcggcgcggc
     1561 cttctttcag ccactgctcc gcgctcgcct tgtcgaagcc gccggcggcg atgtaggcgc
     1621 ctttgaaggt ctcgcgcagc ttggccttga tcgcgtcggg caccttcggc gcgcccatgg
     1681 cgctgtggtc gaggacgtgg atgtagagca ggccgaggct cgagagctcc ttcaccagcg
     1741 cgacgtactg cgcgtcgacc tcggggaagg cgccggtcgc gttgaacgcg ccgtagggcg
     1801 agaggcggat gccgagcttc tccgcgccga tcgcgttggc ggcggcgcgc gcgacctcga
     1861 gcgcgaagcg gttgcggttc tcgatcgagc cgccccagcg gtcggtgcgc ttgttgacgt
     1921 tcgcgttcag gaactgctcg atcaggtagc cgttcgcgcc gtgcagctcg acgccgtcga
     1981 agccggcctg gatcgcgaga cgcgcggcgt gggcgtactc gtcgacggcg tggcggatgt
     2041 ccttctcctc catggcgcgc ggcggggtgt ggggctgcat gcccctgcgg tccgtataca
     2101 tctcgccggg gcagatctcg accgtgggcc cgacgacctc ggcgcggtcg ggcaggttga
     2161 gcgcgtgagt gacgcggccg gtgtgcatga gctgcacgaa gatcttcccg cccttcgcgt
     2221 gcacggcgtc ggtgacgtgc ctccagcccg ccgcctgcgc gtcgttgtac aggcccggga
     2281 tgcgcgcgta gccgagcccg ttcggcgagg gcgacgtccc ttcggtgatg atgaggccgg
     2341 cgcccgcgcg ctgcgcgtag tactcggcca tgagggcgtt cggggtcgcc gcctcgatcg
     2401 cgcggctgcg cgtcatcggc gacatgacca tgcggttctt gagggcaata gctcgcagcg
     2461 agaaaggctc gaagagcatc ggggtggtcc ttgcggagat tgttcttgga gccgatctgg
     2521 gggcgggtcc gccggattgc aagcctttcg ttgtgtttcc cgacggcaca ggtcaaactc
     2581 cggccccaga agccgaaagc gacccaggga gatcgcgtga gaaacgtctt gcttggcctc
     2641 gtggccctgt gcgcgggcgc ggcctgcgcg caatccgacc ctgcgcccgg gcgctcgccg
     2701 ctgtcctcgg cggcgcaggg cgtgtatacg gcggcgcggc cgcggctggt ccagatccgg
     2761 accgtggtca ccgccgccgg ccgccagtcc actctcggct ccgggttcgt cgtcagcggc
     2821 gacgggctcg cgctcaccaa ctaccacgtg gtctcgaagt acgccctcga gcccgcgacc
     2881 taccgcctcg agtacacctc gcccgacggc acgtccggca aggtcgcgct gctcgcgatc
     2941 gacgtcgcca acgacctcgc ggtcgtgcgc ctcgaccgcc gcggcgggcc gtccttccag
     3001 ttcgacgccc gcgccctgca cggcgacctg cccaagggcg agcggctgta ctcgatgggc
     3061 aacccgctcg acctcggctt cgccatcgtc gaaggcacgt acaacggcct ggtcgagcgc
     3121 agctacaacg agcgcatgca cttctccggc gcgctcaacc cgggcatgag cggcgggccg
     3181 accgtcaccg cggacgggca cgtggtcggc gtcaacgtcg cgcggcgctt cggcgccgag
     3241 ctggtcagct tcctcgtgcc cgggcgattc gccgcggcgc tgctcgagcg cgcggcgcgc
     3301 gccgagccgc tgtcgccgaa gaccgtgcgc gccgagatcg gccgccagct cgcctcgtgg
     3361 caggcgggcc tgtacaaggc ggtcggcgcc gcgggcttcc gcgacgcggc gctcgggccc
     3421 taccgggcgc tcgagagctc cgcgccctgg ttccgctgct gggcgagcac caacgccgac
     3481 cgcgtgcccc ggccgcgcgc catcgtcgaa gcgaccagct gcaacaccga aacccagctg
     3541 ttcgtcgccg acgacctggg gaccggccac atcggcgtca cgcacgcgta cgtcaagagc
     3601 ctcgacctca acgcgttcca gttcgccacg ttcctgtcgc agcagtacgc cccgtcgctg
     3661 tcgggctgga accgcaagtg gcagacccgg cagcgctgcc acgacgactt cgtcgagacc
     3721 gccggtccgg cgccggggcc gccgctgcgc gccgtgtggt gcgcgcgcgc ctaccgcgac
     3781 ttcgacgacc tctacgacgt ctcggtgacg gtggtgacgc aggaccgcgg cgacgcggcg
     3841 ctcgtgtcca agctgtccat gcagggcgtc gcctacggcg aggccgcggc gctcgcccgg
     3901 cgcttcgtcg aggcgacgag gatcgcgccg tgatctgggt cgagatcctc tcgcgccacc
     3961 accacgacgt gcaggcgcgc caccgctgcc ccggccccgt cgtccggatc ggccgcggct
     4021 acgacaacga cgtcgtcatc gacgacccgt acgtggcccc gcggcacctg gtcgtgcggc
     4081 gcagcgagaa cggcgagctg gtcgccgagg acctcggcag cgccaacggc acgctggtcg
     4141 agccgggcgc ggaaaggcgc gagcgcgtgg ccctcgacgg cacgcgcgtc ctgcgcatcg
     4201 gccgcacgcg cctgcgcatc cgcgacgcgc tgcaggcggt cgcgcccgag cgcgtgctgc
     4261 ggcccgcgca gcgcacctgg ccggcgacgc tcgcgctgat cgccgcggtg gtgctgctgc
     4321 agctcctcgc cgactggctc ggcgagacca ccgagcccag gctctcgcgc tacgccaagc
     4381 cgctgctcgt cctgccgctg atcgtcgtcg cctggacctc ggcgtgggcg gtcctgtcgc
     4441 gcatcttcgc cggccaggcg cagctcggac gccatctcct catcgccatg tgcggcctgc
     4501 tcgcgatcga ggcgtacgac gccgtccccg agtacgcggc gttcgcgctg tcgtggggcc
     4561 cgctcgcggg cgcccgagtc gctggcctgt ggctgatcat cggggcggtg tgcgtcttcc
     4621 acctgcgggc catcagcccg gcgcggctgc ggctgaaggc ggggctggtc gcggcgctcg
     4681 ccgggatccc gatcgcgctg caggcgctgg cgcagtccga cctgggcgcg ggctacagcg
     4741 gcgagcgcta cgtgcagctg ctcaagccgc ccgcgttccg cttcagcgcg ctgcaggacg
     4801 agagcgcgtt cttcgccggc acggcgcggc tcaaggaccg gctcgaccgc gcccgcacgg
     4861 aagactcgcc ggcggactcg gacgacgact agcgctcggc ctgggtgagc ggcgcgatcg
     4921 tcacctccac ccgccggttc tgcgaccgcc cggcctcggt cgcgttgctc gcgatcgggt
     4981 ggtcctcgcc gcccgcggcg gtcatgatgc gcgcctgggc gatgcccttg ctctcgaggt
     5041 actgggcgac ggaatcggcg cggcgctgcg acagctgccg gttgtacgcc tcgccgccga
     5101 cgttgtcggt gtggcccgcg acctcgagga tggtcttgtc gtacttcttc gcgaccagcg
     5161 cgacgccgtc gagcaccttg tagaactgcc cgttcaggtc cgcgctcgcg gtgcggaacg
     5221 tgatgttgcc cggcatgttg agcacgatgt cgtcgccctt gcgggtgacg gtgacgcccg
     5281 agtcccgcat ctgcgcgcgc agctcgttct cctgccggtc ctggtaggcg ccgatcgcgc
     5341 cgcccgagag cgcgccgacg ccggcgccga tcagcacgcg ctcgcgccgc tgcttcgcgt
     5401 cgcgcccgga gatcatgccg atcgcggcgc cggcgcccgc gccgatcagc gccccgtagg
     5461 tgctgcgcgt ggtcttcgac tcgccgctgt aggggtcggt ggtctggcag ccggcggcga
     5521 ggcccgcggc gagcgcgagc gcgagggtct tcgaggttgg tccgggcacg atgcctcctg
     5581 gagaggggaa cgcccaatgg tagacacacc gcgctgcgat ttatagcgtc gccgcgcccc
     5641 gacacgccga tcggccgatg gatcagcgat ctaatgcatt cggcatgttc cgatgtcgcc
     5701 ttgcgccgac acgattccgg cgccggcgcg ccgtttcccc cggggccggc ccggatggca
     5761 cggcgattgc tcgccagccg tcgggtcgtc aaaacaaaac gcagggggag atcagggatg
     5821 gatccatcgc tcatcggcgc ggagatcgcg tggctgcgcg agctgcgcga cgcgaagcag
     5881 aacaagcagc ccgtccccag cctgccgccg gaagtcgcct tccggctgcg gatgcggggc
     5941 cacgtctcgg ccaacgcgca cggcgaatac gggatcacga tccgcgggca gtcggagctc
     6001 accgagcgcg agcacggact ggccgcggat tctcagcgca gcacgctgta gacctcggcg
     6061 agcgagcgcc gcccttcgga cgcgccgaag acgcgcgccg cgcgggcgag cttctcggcg
     6121 tcgcccgcgg cgatctcctc cgcgagcgcc ttcgcctctg cgcccgtgag cgtgcgcacg
     6181 tgcacgcgcg cgaagcaacg gccggggcga atcagcgcgt cgtccaggtc gccgacgttc
     6241 ggcaggttgg tcgagaagat gatcttccgt ccttgcgcgc gcaccacgcc gtccgcgatc
     6301 gtgagaaagc ggtgcaggtg ctcgttcccg tcggcgcgcg gcttcaggag atggtccgca
     6361 tcctcgacga tgaacgcgtc gtgctcgccg gtgatgaact tcacgtagat ctcgtcggcg
     6421 gcgagcgcct tcatgtcgcc ggtgtagatc gcctgcgcgt gctcgccctt gcggcgcgac
     6481 atctcgccca ggatcgcccg gatgagacgc gtcttgcccg tccccggcgg accttgcagc
     6541 acgagcaccg tctccggggc gtccaggtag cgcgcgatga agccgcgcac gccgccgtgg
     6601 atctcggggt aagcgccatc gtgcagcacg tcgttcgcca tctcctcgat ggacgcgctc
     6661 tcgagcgagt cgcccgaggt gaagtgccaa tcgatcgaga acgtcgggtc ggcgatccgc
     6721 gtcgagcctg ccttcgcgag gagcagcgcc ttcaccgcct ccgcgcg
//
LOCUS       DATDMW010000204         4246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_436508, whole genome shotgun sequence.
ACCESSION   DATDMW010000204 DATDMW010000000
VERSION     DATDMW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436508"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VI319_08745"
     CDS             <1..746
                     /locus_tag="VI319_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEY6863975.1"
                     /translation="GRRTGEMHVALARHTGDAAFDPERITPADFAAWAAQVRASVDQT
                     LAQLAERRAGLSESARTDADALLAARDRMLRHIDELTNEPVQAWKTRFHGDYHLGQVL
                     LTAHDFVIVDFEGEPARPLQERRAKSSPLRDVAGMLRSFGYAAEIALDEATADRPDDR
                     ARLVARAGAWKREATRAFLAGYIDAAHGSPAYPNMPGDARRLICLFMLDKALYEVRYE
                     LGSRPDWIRIPIAGLMELLGDDPPDVGPA"
     gene            757..2916
                     /gene="glgX"
                     /locus_tag="VI319_08750"
     CDS             757..2916
                     /gene="glgX"
                     /locus_tag="VI319_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY6863976.1"
                     /translation="MDLHTRTIASPSAVWRGRPWPLGASWDGEGVNFALYSEHAERVE
                     LCLFDAKGRSETERIELRDRTDFVWHAYLPEARPGTLYGYRVHGPWAPEQGQRFNPHK
                     LLLDPYARMLVGSLRWSDAVLGHRAGGRREDLVADPRDSAACVPKCQVVDPAFTWSED
                     RRPSVPWHDTVIYELHVKGYTQLNPHVPPHLRGTYAGLASAPAVEHLKRLGVTAVELL
                     PVHAFVDEKRLAQAGLRNYWGYNSIGFFAPEPRYSATGTLGEFKTMVQTLHSAGIEVI
                     LDVVYNHTGEGDRLGPTLSFRGIDNAVYYRLERDNPREYVNWTGTGNTLNVPHPAVLQ
                     LVMDSLRYWVTEMHVDGFRFDLATVLARGEHAFDAQGAFLAAVRQDPVVSRVKLIAEP
                     WDLGEGGYQVGAFPAGWSEWNGKYRDAVRSYWKGDGGVMGELASRLSGSSDLYGRRRR
                     GPAASVNFVTAHDGFTLRDLVSYDEKHNEANLEDNRDGESNNRSWNCGAEGASGDPAI
                     LALRARQQRNLLATLLFSQGVPMLLAGDELCRTQHGNNNAYCQDNELSWVDWSMPDEA
                     RALLEFVRRAIALRNAHPLFRRRAFFHGRAERDPAVKDIRWLNPDGRDLSDPDWNRAF
                     ARCLGVFVSGRGLTERDERGNLVEDDDLLLLLSADHDDVPFTLPGAPGTRWDPLVDTD
                     RADGVPAPGALAAGDIFLLRGRSVVLFRSKRTHAPSP"
     gene            2900..>4246
                     /gene="treZ"
                     /locus_tag="VI319_08755"
     CDS             2900..>4246
                     /gene="treZ"
                     /locus_tag="VI319_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEY6863977.1"
                     /translation="MRRRHEMPFGASPKDGGARFRLWAPAAKTVELCLGDETLAMDRD
                     PEGWFETVRPAGGGERYRYRIDSDLLVPDPASRFNPDDVHGPSELVDPLAYEWRDAAW
                     RGRPWEDAVIYELHVGTFSAEGTYAGVERRLDELAGVGVTAVELMPLADFPGTRGWGY
                     DGVLPYAPDASYGRPEDLKRLVDGAHARGLMMFLDVVYNHFGPDGNYLHSYAPRFFTD
                     RYRTPWGAAIDFEDERAVREFFVHNALYWLEEYRFDGLRFDAVHAIHDASRPDIVEEI
                     ADRVRAGPGRERAIHLVLENDANRARYLERGPDGRPRRYTAQWNDDVHHAFHVLLTGE
                     SDGYYSDYADRPAARLGRCLTEGFAYQGEPSPFRDGEIRGEPSAHLPPTAFVSFIQNH
                     DQVGNRALGERISRLAPEAALEAAMAVLLLAPSPPMLFMGDEFAAATPFLFFCDFPG"
BASE COUNT          600 a         1568 c         1496 g          582 t
ORIGIN      
        1 tcggacggcg cacgggagag atgcacgtcg cgctcgccag gcacaccggc gacgccgcct
       61 tcgacccgga gcggatcacg cccgcggact tcgcggcctg ggccgcgcag gtgcgcgcga
      121 gcgtcgatca gacgctcgcg cagctcgccg agcgccgcgc ggggctctcg gagtcggcgc
      181 ggacggacgc cgacgcgctg ctcgcggcgc gcgatcgcat gctgcgtcac atcgacgagc
      241 tcacgaacga gccggtccag gcgtggaaga cgcgtttcca cggcgactac cacctcggcc
      301 aggtgctgct caccgcgcac gatttcgtca tcgtggattt cgagggcgag ccggcgcgcc
      361 cgctccagga gcggcgcgcg aagagcagcc cgctgcgcga cgtcgccggc atgctgcgct
      421 cgttcggcta cgccgccgag atcgcgctcg acgaggcgac ggccgatcgt cccgacgacc
      481 gcgcgcggct cgtggcgcgc gccggcgcgt ggaagcgcga ggcgacgcgc gcgttcctcg
      541 ccggatacat cgatgccgcg cacggctcgc cggcgtaccc caacatgccg ggcgacgcgc
      601 gccggctgat ctgcctgttc atgctcgaca aggcgctcta cgaggtgcgc tacgagctcg
      661 gcagccggcc cgactggatc cggatcccga tcgccggcct catggagctg ctcggagacg
      721 atccgccgga cgtcggcccg gcctaggctc gagccgatgg acctgcacac gcggacgatc
      781 gcctcgccga gcgccgtctg gcgcgggcgg ccctggccgc tcggggcgag ctgggacggc
      841 gaaggcgtca acttcgcgct gtactcggag cacgccgagc gcgtcgagct gtgcctgttc
      901 gacgcgaagg gccggagcga gaccgagcgc atcgagctgc gcgaccgcac cgatttcgtc
      961 tggcacgcgt atctccccga ggcgcgtccg ggcacgctgt acggctaccg cgtgcacggc
     1021 ccgtgggcgc ccgagcaggg ccagcgcttc aatccgcaca agctgctcct cgacccgtac
     1081 gcgcgcatgc tcgtcgggtc gttgcgctgg agcgacgcgg ttctcgggca tcgcgccggc
     1141 ggccgccgcg aggacctcgt cgccgacccg cgcgacagcg cggcctgcgt gccgaagtgc
     1201 caggtggtcg acccggcctt cacctggagc gaggaccggc ggccgagcgt gccctggcac
     1261 gacaccgtga tctacgagct gcacgtcaag ggctacacgc agctcaaccc gcacgtgccg
     1321 ccgcacctgc gcggcaccta tgccggcctc gcgagcgcgc ccgcggtcga gcacctgaag
     1381 cgcctcgggg tgacggcggt cgagctgctg cccgtgcacg cgttcgtcga cgagaagcgg
     1441 ctcgcgcagg cgggcctgcg gaactactgg ggctacaact cgatcggctt tttcgcgccc
     1501 gagccgcgct actcggcgac cgggaccctc ggcgagttca agaccatggt gcagacgctg
     1561 cactcggccg ggatcgaggt gatcctcgac gtcgtctaca accacacggg cgaaggcgac
     1621 cggctcgggc cgacgctctc gttccgcggc atcgacaacg ccgtctacta ccgcctcgag
     1681 cgcgacaatc cgcgcgagta cgtgaactgg acgggcaccg gcaacacgct caacgtcccg
     1741 caccccgcgg tgctgcagct cgtcatggac agccttcgct actgggtgac cgagatgcac
     1801 gtcgacggct tccgcttcga cctcgccacc gtgctcgcgc gcggcgagca cgccttcgac
     1861 gcgcagggcg ccttcctcgc cgccgtgcgg caggacccgg tcgtgtcgcg cgtcaagctg
     1921 atcgcggagc cctgggacct cggcgaaggc ggctaccagg tcggcgcctt cccggccggc
     1981 tggtcggaat ggaacggcaa ataccgcgac gcggtgcgct cgtactggaa gggcgacggc
     2041 ggcgtgatgg gcgagctcgc ctcgcgcctc tcgggctcgt cggacctcta cggtcgcagg
     2101 cgccgcgggc cggcggcgag cgtgaacttc gtgaccgcgc acgacggctt cacgctgcgc
     2161 gacctggtca gctacgacga gaagcacaac gaggcgaacc tggaggacaa ccgcgacggc
     2221 gagtcgaaca accgcagctg gaactgcggc gccgagggcg cgagcggcga tcccgcgatc
     2281 ctcgcgctgc gcgcccggca gcagcgcaac ctgctcgcga cgctgctgtt ctcgcagggc
     2341 gtgccgatgc tcctcgccgg cgacgagctg tgccgcacgc agcacggcaa caacaacgcc
     2401 tactgccagg acaacgagct cagctgggtc gactggtcga tgcccgacga agcgcgcgcg
     2461 ctgctcgagt tcgtgcggcg cgcgatcgcg ctgcgcaacg cgcatccgct gttccggcgg
     2521 cgcgccttct tccacggccg cgccgagcgc gaccccgcgg tcaaggacat ccgctggctg
     2581 aacccggacg ggcgcgacct gtccgacccg gactggaacc gagccttcgc ccgctgcctc
     2641 ggggtgttcg tgtcgggccg cggcctcacc gagcgagatg agcgcggcaa cctcgtcgag
     2701 gacgacgacc tgctgctgct gctcagcgcc gatcacgacg acgtgccgtt cacgctcccc
     2761 ggagcgccgg ggacgcgctg ggatccgctc gtcgacaccg accgcgccga cggcgtgccg
     2821 gcgcccggcg cgctcgccgc cggcgacatc tttctgctgc gcgggcgctc ggtcgtgctg
     2881 ttccgctcga aaaggaccca tgcgccgtcg ccatgagatg cccttcggcg cgtcgccgaa
     2941 ggacggcggc gcgcgctttc gcctgtgggc gccggcggcg aagacggtgg agctctgcct
     3001 cggcgacgag acgctcgcga tggatcgcga tccggaagga tggttcgaga cggtgcggcc
     3061 ggcgggcggc ggcgagcgct accgctatcg catcgacagc gacctgctcg tccccgatcc
     3121 cgcgtcgcgc ttcaacccgg acgacgtcca cgggccgagc gagctcgtcg atccgctcgc
     3181 gtacgagtgg cgcgacgccg cctggcgggg ccgtccgtgg gaagacgcgg tgatctacga
     3241 gctgcacgtc gggaccttca gcgccgaagg cacgtacgcg ggcgtcgagc ggcgcctcga
     3301 cgagctcgcc ggcgtcggcg tcaccgccgt cgagctgatg ccgctcgccg acttccccgg
     3361 cacgcgcggg tggggctacg acggcgtgct gccgtacgcg cccgacgcct cgtacggccg
     3421 gcccgaggac ctgaagcgcc tggtcgacgg cgcgcacgcg cgcggcctga tgatgttcct
     3481 cgacgtggtc tacaaccact tcgggcccga cggcaactac ctgcactcgt atgcgccgcg
     3541 gttcttcacc gaccgctacc gcacgccctg gggcgcggcg atcgacttcg aggacgagcg
     3601 cgcggtgcgc gagttcttcg tccacaacgc gctctactgg ctcgaggaat accgcttcga
     3661 cggcctgcgc ttcgacgcgg tgcacgcgat ccacgacgcg tcgcgtcccg acatcgtcga
     3721 ggagatcgcc gaccgcgtgc gcgccgggcc ggggcgcgag cgcgcgatcc acctcgtgct
     3781 cgagaacgac gcgaaccgcg cgcgctatct cgagcgcggc cccgacgggc ggccgcgccg
     3841 gtacacggcg caatggaacg acgacgtgca ccacgccttc cacgtgctgc tcaccggcga
     3901 gtccgacggc tactactccg actacgccga ccggccggcc gcgcgcctcg ggcgctgcct
     3961 gaccgagggg ttcgcgtacc agggcgagcc ctcgccgttc cgcgacggcg agatccgcgg
     4021 cgagccgagc gcgcatctgc cgccgaccgc gttcgtgtcg ttcatccaga accacgacca
     4081 ggtcggcaac cgggcgctcg gcgagcgcat ctcgcggctc gctcccgagg cggcgctcga
     4141 ggccgcgatg gcggtattgc tgctcgcgcc ctcgccgccg atgctcttca tgggcgacga
     4201 gttcgcggcg gcgacgccgt tcctgttctt ctgcgatttc ccggga
//
LOCUS       DATDMW010000205        15012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1060258, whole genome shotgun sequence.
ACCESSION   DATDMW010000205 DATDMW010000000
VERSION     DATDMW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15012
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060258"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VI319_08760"
     CDS             <1..298
                     /locus_tag="VI319_08760"
                     /inference="COORDINATES: protein motif:HMM:NF023816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863978.1"
                     /translation="EQRQLELAIALAAAPRVLLLDEPAAGLSPEETRRMVELVRALKG
                     RYTIVLIEHKIDIIMSVSDRISVMNFGKLIADGSPAEIRANPEVRRAYLGGMAA"
     gene            295..993
                     /locus_tag="VI319_08765"
     CDS             295..993
                     /locus_tag="VI319_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6863979.1"
                     /translation="MILEVDAVDTYYGLGHILRGLSLGVDEGEVVALLGRNGAGKTTT
                     LRSVTGLTPPRSGRIRFKGRDIAGLDPHRISRLGIALVPETRGIFSYLTARENLAIAR
                     RAASRWQMEDVLGRFPGLRPRLESKGRSLSGGEQQMLAIGRALMTGPDLLLLDEPSQG
                     LAPLVVDAVMSTIRELKKERVSMLLVEQNAEMALALADRVYVIDHGAIVFAGTPAALR
                     ADRGVTATYLGVGA"
     gene            complement(972..1460)
                     /locus_tag="VI319_08770"
     CDS             complement(972..1460)
                     /locus_tag="VI319_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY6863980.1"
                     /translation="MADFISTYRLTHEASLKMIQAGIAKADELGAKVALAVVDQSCQL
                     IALVKMDGAKFFASRTTIKKAITSASQRRPTGYAAEKDVLSMQIRMDGDFTNVPGGFP
                     VVVAGQVIGAVAAGGAKIEEDVIVAKAALAALELPELEDEARDENAAARAREPAQAPT
                     PR"
     gene            1555..2772
                     /locus_tag="VI319_08775"
     CDS             1555..2772
                     /locus_tag="VI319_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6863981.1"
                     /translation="MRRIHVAVTAILAFLASAALPASAQVTAYEGARLIVGDGRVIER
                     ATLLVNGAKIVQAGPDVRTPAGAKRVDLAGKTVMPMIVDTHVHLTTTREGLMKDLRHR
                     AYWGVSAALSLGLDGFELLDVRKQVLPGTARFFSAGRGITMPEPGRTTAPFWVTSAAE
                     GRKAVDEDAAHQVDIIKIWVDTRDGKYQKLTPEIYGAIIDEAHKRKLRVIAHLFDLED
                     AKGLVRANIDAFAHGVRDRDIDDEFVALWKAHPKLVLCPNLPERGVKTDLSWLKAGLA
                     PEAYAKLEKANKDDPKAQAFYGIQARNLARLNAAGARIVLGTDGNKPWGPHEEMQDMV
                     IAGMTPAQVLVAATRNGAEFLKISDAGTISAGKSADFIVLDANPLDDITNTRRIAAVV
                     LRGTAVDRSRAEQ"
     gene            2772..3194
                     /locus_tag="VI319_08780"
     CDS             2772..3194
                     /locus_tag="VI319_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6863982.1"
                     /translation="MPRIVVERSKAEAASRALWKGLVWFNREQGGPFDYSRVVIGVRD
                     DSGRLLGGLILQSYWRESFIELLWLSTRARGAGFGGKLLAEAERRARRRGSRLIHLST
                     YSFQAPKFYEKHGYRRFGGISGSPKGATRHFYVKRLRP"
     gene            3200..4744
                     /locus_tag="VI319_08785"
     CDS             3200..4744
                     /locus_tag="VI319_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6863983.1"
                     /translation="MDTDVLIVGGGPTGLMLAAQLVRRGVRAIIVDRASGPAQQTRAM
                     AVHARTLEIYQHLGIAGRALELGQVGQAANMWANGRRRARIPIGDIGKDISPFPFVLM
                     LGQDDNERILGAHLAQWKVAVQWSTELVSLEQASDGVAATLKQPDGSTRKITAAWLAG
                     CDGGRSTVRERLGIGFPGAPYEHVFFVADTEATGSMVPDDLNVYLLRDGFHLFFPMRG
                     KDRWRVIGILPERLRGRHDLTFEETVPAIRSEADAHVTFKHCSWFSTYRIHHRRAERF
                     RQGRCFLLGDAAHVHSPMGGQGMNTGLQDAYNLAWKLAAVVAGKADARLLDSYEAERI
                     PVAERLLQTTDRAFSLVVADTWTAALFRTRIMARIAATAMRIPRVRRAAFLTISQTGI
                     RYPRSPLSRTSGDLPGDAPQPGDRFPWLRLRLRAGGPVEDLFRALDDTRWHLLVFGDA
                     KADPGDGAERHVVPADPDNARELERARIPAQSFYLLRPDGHVGLAGTRPMPGAIARYL
                     AEAGMR"
     gene            4890..5954
                     /locus_tag="VI319_08790"
     CDS             4890..5954
                     /locus_tag="VI319_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863984.1"
                     /translation="MGNRYKILIMGASYGSLLGTKLALAGHDTHLICLPAEAELINKE
                     GAIVRMPAKGREGLVEINSKKLKGKITAGGTAGVKPGDYDLVALAMQEPQYRSAGVRE
                     LLDAVAKARVPCMSIMNMPPLPYLARIPGVSVGSCRPAYTDATVWDSFDPKLMTLCSP
                     DPQAFRPPEEKVNVLQVRLPTNFKSARFDSDQHTAILRQLEADIEASRFDGLELPVKL
                     KVHDSVFVPLAKWAMLLAGNYRCALKDSVRPIKDAVHSDLEASRSVYSWVVKLCVSLG
                     AKEADMVPFEKYANAALSLLSPSSAARALAAGAPNIERTDRLVQLIGQQRGMRNEIVD
                     QTVATVDGWLERNRAKAKAA"
     gene            complement(5956..6258)
                     /locus_tag="VI319_08795"
     CDS             complement(5956..6258)
                     /locus_tag="VI319_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863985.1"
                     /translation="MFGPMRTSDPARLAHADRVQAWTRARFGLAPDAVISVAQLACGV
                     PGCPPVETVVMFWTADGGHYHFKLFKRLEEVVEDDLPPGWYRDALRVVPGVECSCC"
     gene            complement(6272..7465)
                     /locus_tag="VI319_08800"
     CDS             complement(6272..7465)
                     /locus_tag="VI319_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863986.1"
                     /translation="MSTASVLVPTPPAASAGADARARHPLAQVKADIARARPLCTVFA
                     TSRTDHTLQLYSGLYALRDAGCIRLRQRHARAAAIRSLGAAPAARPRFAKVLENLLVH
                     VEGAGLVFFDVRDSGAWFPDVADRVALYAKRSFRSGAYDGPRGKFVPLGLNYAVVLDR
                     TCADELARALAQLDGSAFSLKRVCLSLVRLVPPIGAALGAPTVSRLSAAPAPGVAPRV
                     IFFARTWEPVEAGDEGFDLLNDTRAACIRALREAFGPRFLGGFSRTPHACRRYPDCVV
                     GADVSTRRADYLRRLRAYPICVATTGLYGSIGWKFAEYVALSKAIVSEPLEFEAPGPM
                     APGENYLEFATPEECVGAVARLFADRDLRSRMMARNREYYLEHGAPDAIVARVLHAAL
                     AAGRF"
     gene            7686..7835
                     /locus_tag="VI319_08805"
                     /pseudo
     CDS             7686..7835
                     /locus_tag="VI319_08805"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
     gene            8185..8463
                     /locus_tag="VI319_08810"
     CDS             8185..8463
                     /locus_tag="VI319_08810"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863987.1"
                     /translation="MVVNTTSQGMVGQEPLDLRLDRLPKNALAADIVYIPLETPFLAA
                     ARRRGNRTVNGLGMLLHQGRPAWKAWFGIEPEVTPELRRMVEKTIQPA"
     gene            complement(8460..10640)
                     /locus_tag="VI319_08815"
     CDS             complement(8460..10640)
                     /locus_tag="VI319_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6863988.1"
                     /translation="MLTRFFRRALRAGADTQPDHAGREAAGLHRRVLIIGVLCLVAVG
                     LVTWRMVVLGQQRVIEHQAIEVAEVVARSALSARSVYTTQIVEKLARDGYGSHAAFED
                     QAGFVPLPAQFLKMFGRFVSRENDGLFSYKPVSKWNIEPTQYLADDFLRWAWPQLEAQ
                     DEAAPTGPIAWKPVWRIEQANGQRILRYLRADPASAQSCVACHNAHEQLDDVRAQRVA
                     NGVTPGKQFRLHQLLGAIDVSIPLDKVEVFAAAQNRITLAVIIGVAGAGVLILGFLIS
                     LDVTRSRRMARELSWQANHDSLTGLASRAAFERRAEELLRQARSADAVHALLFMDLDQ
                     FKLVNDTSGHTAGDELLRELSMVLKGQIRANDMLARLGGDEFGLLLERCPPAKAIEIA
                     EKLHAAVQNFRFVRQGKRFSVGISIGMCQITPATESIASVMSQADLACYAAKDAGRNR
                     IHVFSPTDADITRRRSEMEWVSRVSRAHEEGRISLAVQDARRLNGDGPRAGLYRELLL
                     RMLDEDGKPVAPATLIAAAERFNFMPTIDRWVVKTAFAAIARGDIAIAEDDVVAINLS
                     GTSLNDDHFLDFIREQVRHLSPGVPQQICFEITETAAISSVARARDFIEAVRVMGCRF
                     ALDDFGSGSSSLTYIRQLPLDFLKIEGSFVQSILTDPVAYAMVDAVCHIGRAIHATLV
                     AEWVETDALLARVREMGIDYAQGYAVGPVVPLQLKHGDILPIAA"
     gene            complement(10806..11777)
                     /locus_tag="VI319_08820"
     CDS             complement(10806..11777)
                     /locus_tag="VI319_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6863989.1"
                     /translation="MKLSSLVFAVLTVAAGSASAQAYPVRPITLIVPFAPGGGIDSSA
                     RIQAQAMGELLGQPIVVENMGAAAGTVGSLRVAKAEPDGYIMLMANSGTHAYSQWIYN
                     KRPYDASADFTPVGLVSESPRILVARRDLPVSGLQEFIAYVKANQAKMQFGSAGVGSG
                     THLPCALLNHMLGVNPTHIPYRGAGPAMQDLIAGRIDYMCDTIQTGAVQAKSGKVKGI
                     AVMAAKRSPSIPDLATTGEQGLGGAEVSVWNAFFLPKGAPAPVVRRLNKAMSDMLDSP
                     VIRKRLEDLGLEIVAPERRSPEYLARFLAEEIRRWGVVISDAQIKAD"
     gene            11912..13540
                     /locus_tag="VI319_08825"
     CDS             11912..13540
                     /locus_tag="VI319_08825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6863990.1"
                     /translation="MRVALVHPAGYNFCPGQPDLTLFANRMAPIGILSLASWLDKHGH
                     ETMLLDCLGPRAPKTTEETVQRILAFKPDMVGFSTTTSAFLDGYDIASQIKQKHPEIT
                     TIFGAVHPSSIGGQLLEHFPNADYLCVGEGEGTLVDLADGKALKDVPNLVYRDGGKIV
                     SNPRRARINDLDELPFPAWDKLDGFASGYHLPLFSYIKRYGTNMITSRGCPFTCSFCD
                     RTVYEHKYRHNSAEYTWEHMKLLRDTYGVHHINFYDDLFTASRKRVVELCETLIRKPL
                     GMDWSCAIRVGHNDDEELFALMKRAGALQLSMGIETADPGMMERHKTGLEPEMVRATV
                     RRIQKLGMRVKGLFIMGLPGETPESFRKTSDFVLDLDLDEMNMTKFTPFHGAPMWHEI
                     IANNGKDGNFHEDWRLMNCLNFVYIPEGFQSREQMEDLYNWHILRFYKSKNYERRFRK
                     RLWQHRWTLWHIVKHLPTLLRAKTHFTHEGVSVATQLEMIPLHPAQPTVPLAAGTTSH
                     TAFANVPEQFLPSDKKVIPVRQKSRPDKPERAAA"
     gene            complement(13600..14307)
                     /locus_tag="VI319_08830"
     CDS             complement(13600..14307)
                     /locus_tag="VI319_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY6863991.1"
                     /translation="MTPVELKQREESVSLGELKQQLVDCIRMLERSDIIDYNGHCSIR
                     IAVNRILINTGSCQRSRLTVDDIVTIDLEGNVVEGKGNPPLERHLHMGIYRVRADVRA
                     IVHAHPKWSTFLTMVGAPYQPVYAQGSLVHPLPLLDSPNSINNREMADRLAATLGDRP
                     AALMKSHGAVAVGKDIVEAFVLANYMEENSYRQYMAMQIGKPYAFSEHEIALCREKLW
                     NAGLFKRTWDHFRAKLD"
     gene            complement(14310..>15012)
                     /locus_tag="VI319_08835"
     CDS             complement(14310..>15012)
                     /locus_tag="VI319_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY6863992.1"
                     /translation="IADLAAASRILAAQGVVDGFGHVSMRHPQAPNRYLMARSLAPAL
                     VTPADIVEYDLDSNAVNAEGRPSFLERFIHGEIYKARPDIMAVVHSHSPSVIPFGLVG
                     VPMRAMFHNAGFIAEGVPVFDIREKFGVTDMLVGNGDKGVALAQVMGRKDIVLMRAHG
                     SVACGATLQSAVFRAVYTEVNARIQHWTSALAGGGPIAAVEPEEGKLCDVVNRTAGLR
                     AWDLWRAQVRSQVNW"
BASE COUNT         2350 a         5270 c         5154 g         2238 t
ORIGIN      
        1 cgagcagcgc cagctcgagc tcgccatcgc gctcgccgcc gcgccgcgcg tgctgctgct
       61 cgacgagccc gcggcgggcc tgtcgcccga ggagacgcgg cgcatggtgg agctcgtgcg
      121 cgcgctcaag ggccgctaca cgatcgtgct gatcgagcac aagatcgaca tcatcatgag
      181 cgtgtccgac cgcatctcgg tgatgaattt cggcaagctg atcgccgacg gatcgcctgc
      241 ggaaatccgc gcgaaccccg aggtgcgccg cgcttatctg ggcgggatgg cggcatgatc
      301 ctcgaggtcg atgcggtcga cacttactac ggcctgggac acatcctgcg cggattgtcg
      361 ctcggcgtcg acgaaggcga ggtggtggcg ctgctgggaa gaaacggcgc ggggaagacc
      421 acgacgctgc gctcggtcac gggcctcacg ccgccgcgca gcggccgcat ccgcttcaag
      481 ggccgcgaca tcgccggcct ggacccgcac cgcatctcgc gcctcgggat cgcgctggtc
      541 ccggagacgc gcggcatctt ctcgtatctc acggcgcgcg agaacctcgc gatcgcacgg
      601 cgcgcggcct cgcgctggca aatggaggac gtgctcgggc gcttcccggg cctgcggccg
      661 cgcctcgagt ccaaggggcg ctcgctctcg ggcggcgagc agcagatgct cgcgatcggc
      721 cgggcgctca tgaccggccc cgacctgctg ctgctcgacg agccctcgca gggcctggcg
      781 cccctggtgg tcgacgccgt gatgagcacg atccgcgagc tgaagaagga gcgggtcagc
      841 atgctgctgg tcgagcagaa cgccgagatg gcgctcgcgc tcgccgaccg cgtgtacgtc
      901 atcgaccacg gcgcgatcgt gttcgccggc acgccggccg cgctgcgcgc cgaccgtggc
      961 gtgaccgcga cctacctggg cgtcggcgcc tgagccggct cgcgcgcgcg cgcggccgcg
     1021 ttttcgtccc gggcctcgtc ctcgagctcc ggcagctcga gcgccgcgag cgcggccttc
     1081 gcgacgatca cgtcttcctc gatcttcgcg ccgccggcgg cgacggcgcc gatcacctgc
     1141 cccgcgacca cgacgggaaa tcccccgggg acgttggtga aatccccgtc catgcggatc
     1201 tgcatcgaca gcacgtcctt ctcggccgcg tagccggtgg gacggcgctg cgacgccgag
     1261 gtgatcgcct tcttgatggt cgtgcgcgag gcgaagaact tggcgccgtc catcttcacg
     1321 agcgcgatca gctggcagct ctggtcgacc acggccagcg cgaccttggc gccgagctcg
     1381 tcagccttgg cgatgccggc ctggatcatc ttcaggctcg cctcgtgggt cagtcggtac
     1441 gtcgaaatga agtcggccat gccgatttcg acgctggtcg tcttgctttc gttcccggga
     1501 cagtccccgg caccccaccg ttatactggc gggcccagcg gtcaggaggc ccccatgcgc
     1561 cgaattcacg tcgccgtaac cgccatcctc gcctttctcg catcagcggc actccccgct
     1621 tccgcccagg tcaccgccta cgaaggcgcg cgcctcatcg tcggtgacgg gcgcgtgatc
     1681 gagcgcgcga cgctcctggt gaacggcgcg aagatcgtgc aggccggtcc cgacgtgcgc
     1741 acgccggcgg gcgcgaagcg cgttgatctc gccggcaaga cggtgatgcc gatgatcgtc
     1801 gacacgcacg tccacctcac caccacgcgc gaaggcctca tgaaggatct gcgccaccgc
     1861 gcctactggg gcgtgagcgc ggcgttgagc ctcggcctcg acggcttcga gctgctcgac
     1921 gtgcgcaagc aggtgctccc gggcacggcg cgcttcttca gcgccgggcg cgggatcacg
     1981 atgcccgagc cggggcggac caccgcgccc ttctgggtga cgagcgcggc cgagggccgc
     2041 aaggcggtcg acgaggacgc cgcgcaccag gtcgacatca tcaagatctg ggtcgacacg
     2101 cgcgacggca agtaccagaa gctgacgccg gagatctacg gcgcgatcat cgacgaggca
     2161 cacaagcgca agctgcgcgt gatcgcgcac ctgttcgacc tcgaggacgc caaaggcctg
     2221 gtccgcgcga acatcgacgc cttcgcgcac ggcgtgcgcg accgcgacat cgacgacgag
     2281 ttcgtcgccc tgtggaaggc gcatccgaag ctcgtcctct gcccgaacct gcccgagcgc
     2341 ggcgtgaaga cggacctctc gtggctgaag gcgggtctcg cgcccgaggc gtacgcgaag
     2401 ctcgagaagg ccaacaagga cgacccgaag gcgcaggcct tctacggcat ccaggcgcgc
     2461 aatctcgcgc ggctgaacgc ggccggcgct cgcatcgtgc tcggcaccga cggcaacaag
     2521 ccctggggcc cgcacgagga gatgcaggac atggtcatcg ccggcatgac gcccgcgcag
     2581 gtcctggtgg cggcgacgcg caacggcgcc gagttcctga aaatctccga cgccggaacg
     2641 atctcggccg gcaagagcgc cgacttcatc gtgctcgacg ccaacccgct cgacgacatc
     2701 accaacacgc ggcgcatcgc ggcggtggtc ctgcgcggga ccgcggtgga ccggtcgcgc
     2761 gccgagcaat agtgccgcga atcgtcgtcg agcgctcgaa ggcggaagcc gcttcgcgcg
     2821 cgctgtggaa agggctcgtg tggttcaacc gcgagcaagg cgggccgttc gactattcgc
     2881 gcgtcgtgat cggcgtccgg gacgatagcg gccggctgct gggcgggctg atcctgcagt
     2941 cgtactggcg cgagagcttc atcgagctgc tctggctgtc gacgcgggcg cgcggggccg
     3001 ggttcggcgg caagctactg gcggaggccg agcgccgagc gcgacggcgc ggcagccgcc
     3061 tcatccatct cagcacgtat tcgttccagg cgccgaagtt ctacgagaag cacggctatc
     3121 gccgcttcgg cggaatctcc ggctcgccga agggcgcgac ccggcatttt tatgtgaagc
     3181 gtctgcgtcc ctaagctcca tggataccga tgtcctcatc gtcggcggcg gcccgaccgg
     3241 attgatgctc gccgcccagc tcgtccgccg cggcgtgcga gcgatcatcg tcgaccgcgc
     3301 ctccggcccc gcgcagcaga cgcgcgcgat ggcggtgcac gcccggacgc tcgagatcta
     3361 ccagcacctc ggcatcgccg ggcgcgctct cgagctcggc caggtcgggc aggccgcgaa
     3421 catgtgggcc aacggccggc gccgcgcccg cattcccatc ggcgacatcg gcaaggacat
     3481 cagccccttc ccgttcgtgc tgatgctcgg ccaggacgac aacgagcgca tcctcggtgc
     3541 gcacctcgca cagtggaagg tcgcggtgca gtggagcacc gagctcgtgt ctttggaaca
     3601 ggcgagcgac ggggtcgcgg cgacgctgaa gcagcccgac ggctcgaccc gcaagatcac
     3661 cgcggcctgg ctcgccggct gcgacggcgg gcgcagcacc gtgcgcgagc ggctcggcat
     3721 cggcttcccc ggcgcgcctt acgagcacgt gttcttcgtt gccgacaccg aggcgaccgg
     3781 ctcgatggtg cccgacgacc tgaacgtgta tctgttgcgc gacggcttcc atctcttctt
     3841 ccccatgcgc ggcaaggacc gctggcgcgt gatcggcatc ctgcccgagc gcctgcgcgg
     3901 caggcacgac ctgacgttcg aggagaccgt gcccgcgatc cgcagcgagg ccgacgcgca
     3961 cgtgacgttc aagcactgca gctggttctc gacctaccgc atccatcacc ggcgcgccga
     4021 gcgcttccgc caggggcgct gcttcctgct cggcgacgcc gcgcacgtgc acagcccgat
     4081 gggcggccag ggcatgaaca ccgggctgca ggacgcctac aacctcgcgt ggaagctcgc
     4141 ggcggtcgtc gcagggaaag ccgacgcgcg cctgctcgac agctacgagg ccgagcgcat
     4201 cccggtcgcg gagcgcctgc tgcagacgac cgaccgcgcg ttctcgctgg tggtcgccga
     4261 cacgtggacc gcggcgctct tccgcacccg gatcatggcg aggatcgcgg cgaccgcgat
     4321 gcgcatcccg cgcgtgcgcc gcgccgcgtt cctcacgatc tcgcagaccg gcatccgtta
     4381 cccgcgcagc ccgctctcgc ggacgagcgg cgatcttccg ggagacgcgc cccagccggg
     4441 cgaccgattc ccgtggctgc ggctcaggct gcgcgccggc gggccggtcg aggacctgtt
     4501 tcgcgcgctg gacgacacgc gctggcacct gctcgtcttc ggcgatgcga aggcggatcc
     4561 cggcgacggc gcggagcgtc acgtcgtacc ggccgatccg gacaacgcgc gcgagctcga
     4621 gcgcgcccgg atcccggcgc agtctttcta cctgttgcgt cccgacggcc acgtcggcct
     4681 cgcggggacg cgtcccatgc ccggcgcgat tgcgcgctat ctcgccgagg ccgggatgcg
     4741 atagccgcat gctccgttcg gggcccgggc cgcccggcat ttgcctctcg gcgccctgtt
     4801 ggactacgat cggggattcg ttgtccgtaa cgccgttccg catgccggaa cggcgcagga
     4861 cagcgaccaa gagaggagaa acgtttttca tgggcaaccg ctacaagatt ctgatcatgg
     4921 gcgcgtccta cggctccctg ctcgggacca agctcgcgct cgcgggccac gacacgcacc
     4981 tcatctgcct ccccgccgag gccgagctca tcaacaagga aggcgcgatc gtgcgcatgc
     5041 cggccaaggg ccgcgaaggc ctggtcgaga tcaactccaa gaagctgaag ggaaagatca
     5101 ccgccggcgg caccgcgggc gtcaagcccg gcgactacga tctcgtcgcg ctggcgatgc
     5161 aggagccgca gtaccgctcc gcgggcgtgc gcgagctgct cgacgcggtc gcgaaggcgc
     5221 gcgtgccgtg catgtcgatc atgaacatgc cgcccctgcc gtacctcgcg cgcatccccg
     5281 gcgtctcggt cggctcctgc cgccccgcct acaccgacgc gaccgtgtgg gacagcttcg
     5341 atcccaagct catgacgctg tgcagccccg acccgcaggc gttccgtccg cccgaggaga
     5401 aggtcaacgt gctgcaggtg cgcctgccga ccaacttcaa gtcggcgcgc ttcgactccg
     5461 atcagcacac ggcgatcctg cgccagctcg aggccgacat cgaggcctcg cgcttcgacg
     5521 gcctggagct cccggtgaag ctcaaggtgc acgactcggt gttcgtgccg ctcgcgaaat
     5581 gggcgatgct gctcgccggc aattaccgct gcgcgctcaa ggacagcgtg cgcccgatca
     5641 aggacgcggt gcactccgac ctcgaggcct cgcgctcggt ctacagctgg gtcgtgaagc
     5701 tgtgcgtgtc gctcggcgcg aaagaagccg acatggtgcc cttcgagaag tacgccaacg
     5761 ccgcgctgtc gctgctgtcg ccctcctcgg cggcgcgcgc gctcgccgcg ggcgcgccca
     5821 acatcgagcg caccgaccgc ctggtgcagc tcatcggcca gcagcgcggc atgcgcaacg
     5881 agatcgtcga tcagacggtc gccacggtcg acggctggct cgagcgcaac cgcgcgaagg
     5941 cgaaggccgc ctgatttagc agcagctgca ctccacgccc gggacgacgc gcagcgcgtc
     6001 ccggtaccac cccggcggga ggtcatcctc gacgacctcc tccagccgct tgaacagctt
     6061 gaagtggtag tggccgccgt cggcggtcca gaacatcacg accgtctcga cgggcgggca
     6121 gccgggcacc ccgcaggcga gctgcgcgac cgagatcact gcgtcgggcg cgaggccgaa
     6181 gcgcgcgcgc gtccaggctt gcacgcggtc ggcatgggcg agccgcgccg ggtcgctcgt
     6241 gcgcatcggg ccgaacatgg gcccgagcgt atcagaatcg gcccgcggcg agcgcggcgt
     6301 gcagcacgcg tgccacgatc gcgtcgggcg cgccgtgctc gaggtagtac tcgcggttgc
     6361 gcgccatcat gcgcgagcgc agatcgcggt cggcgaacag gcgcgcgacg gcacccacgc
     6421 actcctcggg cgtcgcgaac tcgaggtagt tctcgccagg ggccatcggg ccgggggcct
     6481 cgaactccag gggctcggag acgatcgcct tcgacagcgc gacgtactcg gcgaacttcc
     6541 agccgatgga tccgtacagt cccgtcgtcg cgacacagat cgggtaggcc cgcaggcgcc
     6601 ggaggtagtc ggcgcggcgc gtgctgacgt ccgcgccgac gacgcagtcc gggtagcggc
     6661 ggcacgcgtg cggcgtgcgc gagaagccgc cgaggaatcg cggaccgaaa gcctcccgca
     6721 gcgcgcggat gcaggccgcg cgcgtgtcgt tgaggaggtc gaagccctcg tcgcccgcct
     6781 cgaccggctc ccaggtgcgc gcgaagaaga tgacccgcgg cgcgaccccg ggcgccggcg
     6841 cggcggagag ccggctcacc gtgggcgcgc ccagcgccgc gccgatcggg ggcacgaggc
     6901 gcacgagcga aaggcacacg cgcttgagcg agaacgccga gccgtcgagc tgcgcgagcg
     6961 cgcgggcgag ctcgtcggcg caggtccggt ccaggaccac ggcgtaattg aggccgagcg
     7021 gcacgaactt gccgcgcggc ccgtcgtagg ccccgctgcg gaagctgcgc ttcgcgtaca
     7081 gggccacgcg gtcggcgaca tccgggaacc acgcgccgct gtcccggacg tcgaagaaga
     7141 cgagccccgc gccttcgacg tgcacgagca ggttctcgag caccttggcg aagcgcggcc
     7201 gcgcggcagg cgccgcaccg aggctgcgga tcgccgcggc gcgcgcatgc ctctgacgca
     7261 gccggatgca gcccgcatcg cgcagcgcgt acaggcccga gtagagctgc agggtatggt
     7321 ccgtccggct cgtggcgaac acggtgcaga gcggccgcgc gcgggcaatg tcggccttca
     7381 cctgcgcgag cgggtggcgc gcgcgcgcgt cggccccggc cgaagcggcg ggcggtgtcg
     7441 gaacgaggac cgaggcggtg ctcatggagg ctcctgcgca gaggacggcg gcgccgcccc
     7501 aggagtggac atctcagctt tagcgcgccg cgtgcgcgga atcgcatcgc gcgacaccgg
     7561 cggcgcgtgg gacgatcggc gcatgcccgc cgacgccgtg cgaaaatgcg ccgatggcac
     7621 accccgaccg cttcctcctc gccggcgtca tgggctggcc ggtgatgcat tcgcgctcgc
     7681 ccatgatgca caaccactgg ttcggcaagc acggcctggc cggcagctac gtgccgctcg
     7741 cgataaggcc cgagggcctc gagcgcgcgt tgcgcgcgct gcatccgctc ggcttcgcgg
     7801 gatgcaatct cactatcccg cacaaggaaa aggcgatgtc gatcgtcgcc gagatcgacg
     7861 cgctcgcggg cgcgatcggg gcgatcagct gcgtcgtggt gaggcccgac gggtcgctcg
     7921 cgggcacgaa caacgactgc ttcggcttcg tccagaacgt ccgggacgag cagcccggat
     7981 ggcgcgccga cgcggggccg atcacggtga tcggagcggg cggcggctcg cgcgcggtgt
     8041 gctacggtct ggcgcgcgaa ggcgcgccgg acatccgcct cgtcaaccgc accttcgcgc
     8101 gcgaaggcgc tcgccgagga gttcggcggg ccgatccggg cgctgccctg gagcgaccgc
     8161 cacgacgcgc tcgagggcgt cgccatggtg gtcaacacca cgagccaggg catggtcggc
     8221 caggagccgc tcgatctccg gctcgacagg ctgccgaaga acgcgctcgc cgcggatatc
     8281 gtctacatcc cgctcgagac gccgttcctc gcggccgcgc gccgccgcgg caatcgcacg
     8341 gtgaacgggc tcgggatgct gctgcaccag ggccgcccgg cgtggaaggc ctggttcggg
     8401 atcgagcccg aggtgacgcc cgagctgcgc cggatggtgg agaagacgat ccagcccgcc
     8461 taggcggcga tcggcaggat gtctccgtgt ttgagctgca gcgggacgac ggggccgacg
     8521 gcgtagcctt gcgcgtagtc gatccccatc tcgcgcacgc gcgcgagcag cgcatcggtc
     8581 tcgacccatt cggccacgag cgtggcatgg atcgcgcggc cgatgtggca gaccgcgtcg
     8641 accatcgcgt aggcgaccgg atcggtcagg atcgactgca cgaagctgcc ttcgatcttg
     8701 aggaagtcga gcggcagctg gcggatgtag gtgagcgagc tggatcccga tccgaagtcg
     8761 tccagcgcga acctgcagcc catgacgcgc accgcctcga tgaaatcgcg cgcgcgcgcg
     8821 acgctcgaga tagccgcggt ctccgtgatc tcgaagcaga tctgctgcgg caccccgggc
     8881 gatagatgcc gcacctgctc gcgaatgaag tcgaggaagt ggtcgtcgtt cagcgaggtg
     8941 cccgacaggt tgatcgcgac gacgtcgtcc tccgcgatcg cgatgtcccc gcgcgcgata
     9001 gccgcgaacg ccgtcttgac cacccaacgg tcgatggtcg gcatgaagtt gaagcgctcg
     9061 gccgcggcga tcagggtcgc gggcgcgacc ggcttgccgt cctcgtcgag catgcgcagc
     9121 agcagctcgc ggtacagacc ggcccgcggt ccgtcgccgt tgagccgccg cgcgtcctgc
     9181 acggccaggc tgatgcggcc ttcctcgtgc gctcggctca cgcgcgagac ccactccatc
     9241 tcggaccgcc ggcgcgtgat gtcggcgtcg gtcggcgaaa acacgtggat gcggttgcgt
     9301 cccgcgtcct tggccgcgta gcaggcgaga tcggcctggc tcatgacgct ggcgatcgat
     9361 tcggtggccg gggtgatctg gcacatgccg atgctgatcc cgacggagaa ccgcttgccc
     9421 tggcgcacga agcggaagtt ctggaccgcg gcgtgcagct tctcggcgat ctcgatcgcc
     9481 ttggcgggcg ggcagcgctc gagcaacagg ccgaactcgt cgccgccgag ccgcgccagc
     9541 atgtcgttcg cgcggatctg gcccttgagc accatgctca gctcgcgcag gagctcgtcg
     9601 cccgcagtgt gcccggaggt gtcgttcacc agcttgaact ggtcgagatc catgaaaagc
     9661 agcgcgtgca cggcgtccgc cgagcgcgcc tggcgcagca gctcctcggc gcgccgctcg
     9721 aacgccgccc ggctcgcgag cccggtcagc gagtcgtggt tggcctgcca gctcagctcg
     9781 cgcgccatgc gccgcgagcg cgtcacgtcg agcgagatca ggaacccgag gatcagcacg
     9841 cccgcgcccg cgacgccgat gatgacggcc agcgtgatcc ggttctgcgc ggccgcgaac
     9901 acctcgacct tgtcgagcgg gatcgagacg tcgatcgccc cgaggagctg gtgcagcctg
     9961 aactgcttgc cgggggtcac gccgttcgcg acgcgctgcg cccggacgtc gtccagctgc
    10021 tcgtgcgcgt tgtggcacgc aacgcacgat tgcgcggagg cgggatcggc gcgcaggtag
    10081 cgcaggatgc gctgcccgtt cgcctgctcg atgcgccaga ccggcttcca ggcgatcggt
    10141 cccgtcggcg ccgcctcgtc ctgcgcttcg agctgcggcc aggcccagcg caggaagtcg
    10201 tcggcgaggt actgcgtggg ctcgatgttc cacttgctga ccggcttgta gctgaacagg
    10261 ccgtcgttct cgcggctgac gaagcgcccg aacatcttca ggaactgagc cggcagcggc
    10321 acgaagccgg cctggtcctc gaacgcggcg tgcgagccgt acccgtcgcg ggcgagcttt
    10381 tcgacgatct gtgtcgtgta cacggagcgc gcgctcagcg ccgagcgcgc caccacctcg
    10441 gcgacctcga tcgcctggtg ctcgatcacg cgctgctgcc cgaggaccac catgcgccag
    10501 gtgaccagtc ccacggccac caggcacagc accccgatga tgaggacgcg acgatgcagc
    10561 ccggcggcct cccgcccggc gtgatcgggt tgcgtgtccg cgccggcgcg cagcgcgcgg
    10621 cggaaaaagc gggtcagcac gacccctccc tgttcgaact gaaaactgga aatgcgcaca
    10681 cgctaacggc gcggcgctaa ttgttatttc ttgaaactag ccggttttcg tgaccaaaga
    10741 cagggaatcc ggcagaaaaa cgacaacttg ttgcgcgcgc cgctccaagc ggccggcagg
    10801 ttgcctcaat cggccttgat ctgggcgtcg ctgatcacga cgccccagcg tcggatctcc
    10861 tcggcgagga agcgcgcgag gtattccggg ctgcggcgct ccggcgcgac gatctcgagc
    10921 cccaggtcct ccaggcgctt gcggatcacc gggctgtcga gcatgtcgct catcgccttg
    10981 ttgaggcggc gcacgaccgg ggcgggcgcg cccttcggca ggaagaacgc gttccagacg
    11041 ctgacctcgg cgccgccgag cccctgctcg cccgtggtgg cgagatccgg gatgctcggc
    11101 gagcgcttcg ccgccatcac cgcgatgccc ttcactttcc ccgacttcgc ctggacggcg
    11161 ccggtctgga tcgtgtcgca catgtagtcg atgcgccccg cgatgaggtc ctgcatcgcc
    11221 gggcccgcgc cgcgataggg aatgtgcgtg gggttgacgc cgagcatgtg gttgagcagc
    11281 gcgcacggca ggtgcgtgcc cgagccgacg cccgccgagc cgaactgcat cttcgcctgg
    11341 ttcgctttca cgtaggcgat gaactcctgc aggccgctca cgggaagatc cctgcgcgcg
    11401 acgagaatgc gcggcgactc cgagaccagc ccgacgggcg tgaaatcggc gctcgcgtcg
    11461 tagggccgct tgttgtagat ccactggctg tacgcgtgcg tcccgctgtt cgccatcagc
    11521 atgatgtagc cgtcgggctc ggccttggcg acgcgcaggc tgcccaccgt gcccgccgct
    11581 gcgcccatgt tctcgaccac gatcggctgc ccgagcagct cgcccatggc ctgcgcctgg
    11641 atgcgcgcgc tcgagtcgat gccgcctccc ggagcgaacg gcacgatcag cgtgatgggg
    11701 cgaaccggat acgcctgggc agaggcgctt ccggcggcga cggtcagcac ggcgaagacg
    11761 aggctcgaga gcttcattgc gcgggttcct ggcgggggac cgatcgtgcc gaaatctatt
    11821 ccaaatggcc tgcgcgaggc aagggctccc tttgcgctcg cgacgcttcg tgctacagtc
    11881 gtcccgcttc gtatcccgag ggaggcgcga aatgcgcgtc gcactggtcc acccggccgg
    11941 ttacaacttc tgccccgggc agccggacct caccctgttc gcgaaccgga tggccccgat
    12001 cggcatcctg tcgctcgcgt cgtggctcga caagcacggc cacgagacga tgctgctcga
    12061 ctgcctcggg ccgcgcgccc cgaagaccac cgaggagacg gtgcagcgca tcctcgcgtt
    12121 caagcccgac atggtcggct tctcgaccac gacctcggcg ttcctcgacg gctacgacat
    12181 cgcctcgcag atcaagcaga agcaccccga gatcacgacc atcttcggcg cggtgcatcc
    12241 ctcctcgatc ggggggcagc tgctcgagca cttcccgaac gccgactacc tgtgcgtcgg
    12301 cgagggcgaa ggcacgctcg tggacctcgc cgacggcaag gcgctgaagg acgttcccaa
    12361 cctcgtctac cgcgacggcg gcaagatcgt ctcgaacccg cgccgcgcgc gcatcaacga
    12421 cctcgacgag ctgcccttcc ccgcctggga caagctcgac ggcttcgcga gcgggtatca
    12481 cctgccgctc ttcagctaca tcaagcgcta cggcacgaac atgatcacca gccgtggctg
    12541 cccgttcacg tgcagcttct gcgatcgcac ggtgtacgag cacaagtacc gccacaactc
    12601 ggccgagtac acctgggagc acatgaagct cctgcgcgac acctacggcg tgcaccacat
    12661 caacttctac gacgacctgt tcaccgcgag ccgcaagcgc gtggtcgagc tgtgcgagac
    12721 gctgatccgc aagccgctcg gcatggactg gagctgcgcc atccgcgtcg gtcacaacga
    12781 cgacgaggag ctgttcgcgc tcatgaagcg cgccggcgcg ctgcagctgt cgatgggcat
    12841 cgagaccgcc gacccgggga tgatggagcg gcacaagacg ggcctggagc ccgagatggt
    12901 ccgcgccacg gtccgccgca tccagaagct cggcatgcgc gtgaagggcc tgttcatcat
    12961 ggggctgccg ggcgagacgc ccgagagctt ccgcaagacc tcggacttcg tcctcgacct
    13021 cgacctcgac gagatgaaca tgacgaagtt cacgccgttc cacggcgcgc cgatgtggca
    13081 cgagatcatc gccaacaacg gcaaggacgg aaattttcac gaggactggc gcctgatgaa
    13141 ctgcctgaac ttcgtgtaca tccccgaggg cttccagtcg cgcgagcaga tggaagacct
    13201 ctacaactgg cacatcctgc gcttctacaa gagcaagaac tacgagcgcc gcttccgcaa
    13261 gcgcctgtgg cagcaccgct ggaccctctg gcacatcgtc aagcacctgc cgacgctgct
    13321 cagggcgaag acgcacttca cgcacgaggg cgtctcggtc gcgacgcagc tcgagatgat
    13381 cccgctgcac ccggcgcagc cgacggtccc gctcgccgcg ggcaccacct cgcacacggc
    13441 cttcgccaac gtgccggagc agttcctgcc ttcggacaag aaggtcatcc cggtgcggca
    13501 gaagtcgcgg ccggacaagc ccgagcgcgc ggcggcgtag gtcatctcag aaacgaaaac
    13561 ggccgggatc cccggccgtc tttgtttgcg gcgcgccgct cagtccagct ttgccctgaa
    13621 gtgatcccag gtgcgcttga acaggcccgc gttccagagc ttctcgcggc acagcgcgat
    13681 ctcgtgctcg ctgaacgcgt acggcttgcc gatctgcatc gccatgtact ggcggtacga
    13741 gttctcttcc atgtagttcg cgagcacgaa cgcctcgacg atgtctttcc cgaccgcgac
    13801 ggcgccatga gatttcatga gcgccgcggg ccggtcgccc agcgtcgccg cgaggcggtc
    13861 ggccatctcg cggttgttga tcgagttggg cgagtcgagc agcgggagcg ggtgcacgag
    13921 cgagccctgc gcgtacacag gctggtaggg cgcaccgacc atcgtcagga acgtcgacca
    13981 tttcgggtgc gcgtgcacga tcgcgcgcac gtccgcgcgc acgcggtaga tgcccatgtg
    14041 caggtggcgc tcgagcggcg ggttgccctt gccttcgacg acgttgccct cgaggtcgat
    14101 ggtcacgatg tcgtcgaccg tgagccggct gcgctggcac gaccccgtgt tgatcaggat
    14161 gcggttcacc gcgatgcgga tgctgcagtg gccgttgtag tcgatgatgt ccgagcgctc
    14221 gagcatgcgg atgcagtcga cgagctgctg cttcagctcg cccagcgaga cgctctcttc
    14281 gcgctgcttg agctcgaccg gggtcatggt caccagttta cctggcttcg gacctgcgcg
    14341 cgccagaggt cccaggcgcg caggcccgcg gtgcggttga cgacgtcgca gagcttgcct
    14401 tcctcgggct cgaccgccgc gatcgggccg ccgcccgcga gcgcgctcgt ccagtgctgg
    14461 atgcgcgcgt tgacctcggt gtagaccgca cggaacaccg ccgattgcag cgtcgctccg
    14521 caggcgacgc tgccgtgcgc gcgcatgagc acgatgtcct tgcggcccat cacctgcgcg
    14581 agcgcgacgc ccttgtcgcc gttgcccacc agcatgtcgg tcacgccgaa cttctcgcga
    14641 atgtcgaaca cgggcacgcc ctccgcgatg aatcccgcgt tgtggaacat ggcccgcatc
    14701 ggcacgccga ccagcccgaa cgggatcacc gacggcgagt ggctgtgcac cacggccatg
    14761 atgtcgggcc gcgccttgta gatctcgccg tggatgaagc gctcgaggaa gctcgggcgg
    14821 ccctcggcgt tcaccgcgtt gctgtcgagg tcgtactcga cgatgtcggc cggcgtcacc
    14881 agcgccggcg cgagcgagcg cgccatcaga tagcggttcg gcgcctgcgg atgccgcatc
    14941 gagacgtgtc cgaagccgtc gaccacgccc tgggccgcga ggatccggct ggcggcggcg
    15001 aggtcggcga tc
//
LOCUS       DATDMW010000206         3843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2181112, whole genome shotgun sequence.
ACCESSION   DATDMW010000206 DATDMW010000000
VERSION     DATDMW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181112"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VI319_08840"
     CDS             <1..628
                     /locus_tag="VI319_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863993.1"
                     /translation="MSEPKCPARGDYIVMQNVVLTPLHMRSVWHYRHRVDDTVSPPYE
                     AFWDSGGINVGYSYATAKSFFGDGFSNWVKHTGLHFDTSAFEHWTIRSAVLKLRVDLS
                     NVGTDRHEDHTTSCVTRVEEAREYWWDHPNAKAMIQTVPGAENRGVQNGPDLVLDVTS
                     IVRGWAQFPDRNFGLVLGNDKTGVDVFLDEACLTQYVPTSARLEVDFY"
     gene            645..2345
                     /locus_tag="VI319_08845"
     CDS             645..2345
                     /locus_tag="VI319_08845"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEY6863994.1"
                     /translation="MSRNALKIPLAAIAIAAAGAACAQGAPSSLPRGNAASPFGAAPQ
                     DSQAPAEWRPGQRTTTVAPAQTPPISARSAAKAPVPPPIRIAPGPRPFVVASACRAGT
                     PLVTLNVVLRNVGSAAVSSRLPLVARDEGAVAWSGRAPVPNLAPGASANVSIPLYPPT
                     YAPRADGTHRFTVASALGSAAAPIAVTLPARVCAGLASRFKASAVLAKSVAAAPAQTS
                     AAGYTTRRAGATAIQSQNGAITPEELRLAGRVRRNRPSPPSDVTSTADPKVCTDHGGL
                     GGGLACAALLPQGRLALVWTWSGTAADGFNVYRVDGGQRARVGRQANGKDVTLYVIDP
                     VPADGYSGKCYAVSATRGNEESDLSVPACTDAARVMQTATLTPGAMNSSRRERLASTG
                     ALGIAPEEWVSDSGEYAAGYDYSRTITVIGDTSHNGYGRLGILFDTGSISSTAVYTAR
                     LKLTVDHTKVNQDEDPRLNSNHSTSCVARVGLGTANWWKYPGDWIEFSTATTPRIDQV
                     GPDVYIDVTDIVRGWAQAPKSNFGFVLMGDNESLSGFTNAACWSVYDPASVQLQVQFF
                     "
     gene            2345..2617
                     /locus_tag="VI319_08850"
     CDS             2345..2617
                     /locus_tag="VI319_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863995.1"
                     /translation="MDGLGWSVALGLTALALIAVAARLLIGRTFALTLAALALIAGLA
                     WLGRAHLGDAAAMVLWSRIPFTAMLIPLGEVLVVLSLVVLYARRRY"
     gene            2624..3121
                     /locus_tag="VI319_08855"
     CDS             2624..3121
                     /locus_tag="VI319_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863996.1"
                     /translation="MKILFWAGLTAVNVATPLAVVSCTRARPAPCAACVAVALAAVAA
                     VWALWAGPIDSLQLTRVFGSGEKALAFIFAPWLATVLAAGMILAFPGWASRAPPRSAQ
                     RALDGRAGQIGHVSLCVLALLGVVYAFWSELDEAACWISLVTLQSAGLAWLSAGRRVA
                     LPPSA"
     gene            3136..3543
                     /locus_tag="VI319_08860"
     CDS             3136..3543
                     /locus_tag="VI319_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6863997.1"
                     /translation="MRAVFALVIGVPGTLVFVPVIALALVTFVTSVLGVMIGNTAGSA
                     PMRGALLALFWSAGGLAGLIGFWAWVFLRPAAPWKTRIAIACCVGAGMCAVVPFALGG
                     ELLGSLALIGLAAGAAICAWLVFPASPLNPDAP"
     gene            complement(3540..>3843)
                     /locus_tag="VI319_08865"
     CDS             complement(3540..>3843)
                     /locus_tag="VI319_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6863998.1"
                     /translation="LEQHPWVAMSVVQAFEQAKELGYRRMRDPRNLALAWVREAREEQ
                     QAVLGKDAWTCVLVEPNSKAIEAVVRYAYDQGMIKKKPRVEELFFAPSLQEIQHYL"
BASE COUNT          518 a         1342 c         1351 g          632 t
ORIGIN      
        1 gatgagcgag ccgaagtgcc cggcgcgcgg cgactacatc gtgatgcaga acgtcgtgct
       61 cacgccgctg catatgcgct cggtctggca ttaccgccac cgtgtcgacg acaccgtcag
      121 ccctccgtac gaggcgttct gggactcggg cggaataaac gtgggctatt cctacgcgac
      181 ggcgaaatcg ttcttcggcg acgggttcag caactgggtc aagcatacgg ggctgcactt
      241 cgacaccagc gccttcgagc attggacgat ccgctcggcc gtgctcaagc tgcgcgtcga
      301 tctcagcaac gtcggcaccg accgccacga ggatcacacg acgtcgtgcg tgacccgggt
      361 cgaggaagcg cgcgaatact ggtgggacca tcccaacgcg aaggcgatga tccagacggt
      421 gcccggcgcc gagaacaggg gagtccagaa cggcccggat ctcgtgctcg acgtgacctc
      481 gatcgttcgc ggctgggcgc aattcccgga caggaacttc gggctcgtgc tcggcaacga
      541 caagaccggt gtcgacgtgt ttctcgacga ggcgtgcctg actcagtacg tgccgacctc
      601 ggcgcggctc gaggtcgact tctactgagg atctggagca ggatatgagc cgcaatgcac
      661 tgaaaatccc gctcgcggcg atcgccatcg ccgctgccgg cgcggcgtgc gcgcaaggtg
      721 cgccgagctc gctcccgcgc ggcaacgcgg catcgccgtt cggcgctgcg ccgcaggatt
      781 cgcaggcgcc ggcggagtgg cggccgggcc agcggaccac cacggtcgcg ccggcacaga
      841 cgccgccgat ctccgcgcgg tccgccgcaa aggctcccgt gccgcctccg atccggatcg
      901 cgccggggcc gcgtcctttc gtcgtggctt ccgcgtgccg tgccgggacg ccgctggtca
      961 cgctcaatgt cgtgctgcgc aacgtcggca gcgcagccgt gtcgagccgc ctgccgctgg
     1021 tcgcgcgcga cgagggcgcg gtcgcctggt cggggagggc gccggtgccg aatctggcgc
     1081 cgggcgcgtc cgcgaacgtc tcgattccgc tgtatccgcc gacctatgcg ccgcgcgcgg
     1141 acggcacgca ccgcttcacc gtcgcttccg cgctcggctc ggccgcggcg cccatcgccg
     1201 tcaccctgcc ggcccgcgtg tgcgcggggc tcgcgtcccg gttcaaggcg agcgctgtcc
     1261 tcgcgaagtc ggttgcggcg gcgccggcac agacgagcgc ggcgggctac acgacgagac
     1321 gcgcgggcgc gaccgccatt cagtcgcaga acggagccat caccccggag gagctcaggc
     1381 tcgcgggccg cgtgcgccgg aaccggccgt cgccgccgtc ggacgtgacg agcaccgcgg
     1441 acccaaaagt ctgcaccgac cacggcggtc tcggcggcgg gctcgcgtgc gcggcgctcc
     1501 tgccgcaggg tcgcctcgcg ctggtgtgga cgtggtcggg cacggcggcc gacggcttca
     1561 acgtctatcg cgtcgacggt ggccagcgcg cgcgcgtcgg ccgccaggcg aacggcaagg
     1621 acgtgacgct ctacgtgatc gatccggtgc ccgccgacgg ctactcggga aagtgctacg
     1681 cggtgagcgc gactcgcggc aacgaggagt cggacctgag cgtgcccgcg tgcaccgacg
     1741 ccgccagggt catgcagacc gcgaccctga cgccgggcgc gatgaattcc tccaggcgcg
     1801 agcgtttggc ttccaccggg gccctgggaa tcgctcccga ggaatgggtt tcggactcgg
     1861 gagaatacgc cgccggatac gactacagtc gcaccatcac cgtgatcgga gacacgtcgc
     1921 acaacggtta tggccgtctc ggcatcctgt tcgataccgg ctcgatctcg agcacggctg
     1981 tctataccgc gcgcctgaag ctcacggtcg accatacgaa ggtcaatcag gacgaggatc
     2041 ccaggctcaa cagcaaccat tcgacgtcct gcgtcgcgcg cgtcggcctg ggcacggcga
     2101 actggtggaa atatccgggc gactggatcg aattttccac cgcgaccacg ccgagaatcg
     2161 accaggtcgg acctgacgtg tacatcgacg tcaccgatat cgtgcggggg tgggcccagg
     2221 ccccgaaatc gaatttcggg ttcgtgctga tgggggacaa cgagagtctc tcggggttca
     2281 ccaacgccgc ctgctggagc gtgtacgacc ccgcgagcgt gcagctgcag gtgcagttct
     2341 tctgatggac ggcctcggct ggagcgtcgc gctcgggctg acggcgctcg cgctgatcgc
     2401 cgtcgcggcg cgcctgctga tcggccgcac cttcgccttg acgctcgccg cgctggccct
     2461 gatcgccggg ctggcgtggc tcgggcgcgc gcacctcgga gacgccgcgg cgatggtctt
     2521 gtggagccga atcccgttca ccgcgatgct gattccgctg ggcgaggtgc tcgtggtgct
     2581 atcgctcgtc gtgctgtacg cccgacggcg ctactgatcc atcgtgaaga tactattctg
     2641 ggccggcctg accgcggtga acgtcgccac gccgctcgcc gtcgtgtcgt gcacgcgcgc
     2701 gcgcccggcg ccgtgcgccg cgtgcgtggc cgtcgcgctc gcggccgttg ccgcggtgtg
     2761 ggcgctctgg gccggcccaa tcgactcgct gcagctcacg cgcgtcttcg gaagcggcga
     2821 gaaggcgctg gcgttcatct tcgcgccctg gctcgccacc gtgctcgcgg cggggatgat
     2881 cttggccttc cccggctggg cgtcgagggc gccgccgcgg tccgcgcagc gcgcgctcga
     2941 cgggcgcgcg gggcagatcg ggcacgtctc gctttgcgtg ctcgcgctcc tcggcgtggt
     3001 ctacgccttc tggagcgaac tcgacgaagc ggcgtgctgg atctcgctgg tcacgctgca
     3061 gtcggccggc ctcgcgtggt tgagcgcggg ccggcgcgtc gcattgccac cgtccgcatg
     3121 acgcgccggg cgctggtgag agcggtcttc gccctcgtca tcggcgtgcc cgggacgctg
     3181 gtgttcgtgc cggtcatcgc gctggcgctc gtcaccttcg tcaccagcgt gctcggcgtg
     3241 atgatcggca acacggcagg gtcggcgccc atgcgcgggg cgctgctcgc gctcttctgg
     3301 agcgcgggcg gcctcgccgg gctgatcggg ttctgggcct gggtgttcct gcgtcccgcg
     3361 gcgccgtgga agacgcggat cgcgatcgcc tgctgcgtcg gcgccggcat gtgcgctgtc
     3421 gtgcctttcg cgctcggcgg cgagctgctc ggcagtctcg cgctgatcgg gctggccgcg
     3481 ggagcggcga tctgcgcgtg gctggtcttc cccgcgtccc cgctcaatcc cgacgccccc
     3541 tagaggtagt gctggatctc ctgcaggctc ggcgcgaaga acagctcctc gacccgcggc
     3601 ttcttcttga tcattccctg gtcgtacgcg tagcgcacca cggcctcaat cgccttgctg
     3661 ttcggctcga cgaggacgca ggtccacgcg tccttgccga ggacggcctg ctgctcctcc
     3721 ctcgcctcgc gcacccacgc gagcgcgagg ttgcgcgggt cgcgcatgcg gcggtagccg
     3781 agctccttcg cctgctcgaa cgcctgcacc acgctcatcg cgacccaggg atgctgctcg
     3841 agg
//
LOCUS       DATDMW010000207        16711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_934930, whole genome shotgun sequence.
ACCESSION   DATDMW010000207 DATDMW010000000
VERSION     DATDMW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934930"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VI319_08870"
     CDS             <1..922
                     /locus_tag="VI319_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HEY6863999.1"
                     /translation="PAPGFCPYRSLLATGAALFGRPEFKAKASALDDKTRWLLGDADE
                     RYAAVDDSRARLPVRREFPEGGYRVLGTDFETAREIRLVVDCGPLGYTRIAAHGHADA
                     LAFTLSVGGLEFLVDPGTYAYHTQGRWRQYFRGTSAHNTLRVDGESQSQPGGSFLWLA
                     KAEARCDAWDTSDERDVFEGRHDGYRRLPDPVVHRRRVVLEKRERRILVDDGVEARGA
                     HRVEAFFHCHEACAVEPVPGGFALRRDGRTVMLLFPDAGVSDTSVLRASVDPIGGWVS
                     RRFDDKVPATTIVRRATTAGNAVLRTLIVC"
     gene            1061..2386
                     /locus_tag="VI319_08875"
     CDS             1061..2386
                     /locus_tag="VI319_08875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY6864000.1"
                     /translation="MKISVMGLGYVGAVSAACLAKQGHEVIGVDPQRAKVDLINEGRT
                     PVIERDLGEIIAEAVGDGRLRATSDVVAAVQHTDLAFVCVGTPSLPNGGIDLRYMRRV
                     CEEIGQALRGHAGAPTIVIRSTMLPGTMRDVVIPTLESGSGKRAGAEFGVCINPEFLR
                     ESTAVHDYFNPPKTVIGEVNRASGDVLASLYAGLSAPMIRTDVETAEMVKYADNCWHA
                     VKVGFANEIGNICKAVDVDSHRVMEIFCQDTKLNLSSYYLKPGFAFGGSCLPKDLRAL
                     LHKAKSLDVDVPILASVLPSNELQIDRAVRAVIDAAAKARSRKIGVLGFSFKAGTDDL
                     RESPVVELTERLIGKGYELRIYDRNVSLASIHGANRDYILNHIPHISRLMVGSIGEVL
                     DHGATIVIGNADPDFRGVPKLLGDGQAVIDLVRVADSRSVSGVYEGICW"
     gene            2394..3296
                     /locus_tag="VI319_08880"
     CDS             2394..3296
                     /locus_tag="VI319_08880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6864001.1"
                     /translation="MTAVYTPVPGAGNDDAPLPKALRAPIARGELRDEAQLREHYLIE
                     RELADRLRRAAPDERRRLYAQVYDELYRRVPHHPMNRPSGHPRAAGVERDLVFLRRFL
                     RPTTAFLEIGAGDCALACRVAAVVRRVVAVDVSEEIMRLAPSAMNLERALSDGTSIPV
                     RAGSIDVAFSDQLMEHLHPDDAAEQLRNIHRALAPGGVYLCITPNRLYGPRDISGYFD
                     DVATGFHLREYTGGELAPLFRSVGFSRLRFYAGARGWYLPVPAAPVHAAETALEMLPA
                     PARRRVAGVAPVRALLGLRVAAIK"
     gene            3311..4525
                     /locus_tag="VI319_08885"
     CDS             3311..4525
                     /locus_tag="VI319_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6864002.1"
                     /translation="MAKRVLILVENLPSPFDRRVWQEATALRDAGWTVSIVCPTGRGY
                     EQRFERLDGIDIHRYPLPVEASGALGYALEYGAALFWSFVLSWKVFFGRGFDVVHACN
                     PPDLFFLVGAVFKLFGRKFVFDHHDLNPELYEAKFGRRDLFYRAMLALERWTFRTADV
                     SIATNESYRRIAIERGGMAPGRVFVVRSGPSLERMKIVPPVETLKCGRRYLVGYVGVM
                     GRQEGIDLLLRAAREIVHERGRGDVHFGLVGGGTSLEEMKALARELRVSDHVTFTGRV
                     PDAALLAMLNTADVCVNPDVANEMNDKSTMNKIMEYMSLGKPIVQFDLTEGRFSAQEA
                     SLYARRNDPADLAAKIRELLDDPERRARMGAFGRRRVEQELEWRHEVPKLLAAYEALR
                     SPSRAAPAAQRY"
     gene            complement(4519..4713)
                     /locus_tag="VI319_08890"
     CDS             complement(4519..4713)
                     /locus_tag="VI319_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864003.1"
                     /translation="MPRDSFICLGCKKPSRLVEVHDADKAPYVVCAHCGTRNKVLRLK
                     TFLSGAPEYAVVGIRLDDDQ"
     gene            4899..5270
                     /locus_tag="VI319_08895"
     CDS             4899..5270
                     /locus_tag="VI319_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6864004.1"
                     /translation="MKVLIADDSQIVRERLAYLLGDVEGVEIVGQAEDAVEGSNLAEA
                     LKPDVAILDVRMPRGSGLDVLRSIKSHHPSATVIILTNFVDPEARQLCLARGADYFFD
                     KSIEFDKAVDVLRGLAQPETP"
     gene            5276..7756
                     /locus_tag="VI319_08900"
     CDS             5276..7756
                     /locus_tag="VI319_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864005.1"
                     /translation="MKFEDGLDRRSILAALRAYRKGDFSARLPLDLVGVDGEIAQVFN
                     DLVETSAACAAEVARVRDEVGGQGRLAQRAGVPAAAGGWASLLEGVNALLDDSGRPLA
                     EIARVVQAVTHGDLSRAMALEIDGRPLRGELLRIAQAVNAMVRHLAAQDWLKSNLARF
                     TRMLQGHGDLVTVSRLVLSELAPLVSVQRAVLYGRAKRAAEPRLDLLATYATSSDQAV
                     PPSLRIGEGLVGQCAYEKKRLLLEEVPADYPRASSGLGSVRPLNIVIVPVLFEGEIEA
                     VIELASFRKFGDTQLAFLDQLAESIGIVFNTIEANMRTVELLERSQALTRELQGQQDG
                     LIKTNDRLERQADALRKSESLLLKQQDELRRSNEQLAERAKALSEQMKQVELRNKEVE
                     QARAALEEKAEQLALSSRYKSEFLANMSHELRTPLNSLLILAKIFADNAASNLTPKQV
                     EYAQTIHAAGTDLLSIINDILDLAKIESGTVTLEIGHEPFSQLRDYVERTFRQVAIGK
                     RLDFAVTLDPALPEAIETDSKRLQQVLKNLLSNAIKFTERGRVSLHIAPARSGWLPGN
                     ARLDGASHVVSFAVTDTGIGIAESKQKIIFEAFRQADGSTGRRYGGTGLGLSISRELA
                     RLLGGEIRVSSAPGKGSTFVVYLPVMHKATEGEPAPAAARAPAFSAAYASRSAPRAAP
                     RPQPQVPAAVMREVKEELAGRKVLVVDDDIRNIFAMTASLEQLGMTVAHAESGKEGIE
                     VLKSEPGIDVVLMDIMMPDLDGYDTIRIIRGLDGLRDVPIIAVTAKAMKEDRERCLQA
                     GANDYISKPVNMEQLSLMLRASLARSLG"
     gene            7761..11039
                     /locus_tag="VI319_08905"
     CDS             7761..11039
                     /locus_tag="VI319_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864006.1"
                     /translation="MAVRTSKANILVVDDELHSLIALQHLLSGPDRNVVPARSGREAL
                     RHVLKNDFAVILLDVRMPDMDGFETAGLIRQLRRCRHTPIMFLTAAVEDTQSMFRGYE
                     VGGADYILKPVDPAVLTSKVAVFVDLYGKSAELTTQIHKRKEAERELYRTNEDLETKI
                     RERTASLIAANDLLQKEIARRERIEERLRRSEAEARRLSLVASLTENAVLIKDADDRV
                     EWVNESFTRMFGYPLDEVRGRRTDEVFPREAGDLGVAAGTDVEGGYRAERVRRSRGGR
                     KYWLAVECRPILDDGGAPAGFIEIATDITERKLSEESLRESEARKTAILESALDCIVT
                     MDHKCRILEFNPAAEKAFRCARAEAVGRYAPGLLLPAATRKAQLRLLGTLLAPGDPAD
                     RARRVEITARRCDGSEFPAELTIAMATLGGRPFFAAYVRDITARKEAEADLHKAKQAA
                     EAANLAKSQFLANMSHEIRTPMNAIIGMTELALQTWLNGEQREYLGLVKDSGETLMKL
                     INEILDFSKIEAGRLEVETIPFSLRESVGDTMKTLGFQAHKKKGLELAYDIAPEVPDA
                     VVGDPVRLRQIVINLVDNAIKFTERGEIVMRVSCEGIDAGGVVCRFAVRDTGVGIARE
                     KQAAIFAPFLQADASTTRIFGGTGLGLTISARLVEMMGGEIWVESEPGAGSIFHFTVR
                     FGLQRAAEIPFENSAADFAGARALVAEDHPVSRGFIVSTLRQWNIEVVEAASAAEAME
                     SIARSAGAPFHLALIDDALPGGDAAGLLARLRHSGGAACPAVIVLASALRREGAEAGA
                     ALIAKPVKPSELLAAVRAALIGDAGAQAASAPPAAASARERLRGLLVEDNPVNHKLAV
                     HVLEKDGHDVVAAENGEVAVSVLDSSTFDLVLMDVQMPKMDGIEATRAIREKERSSGA
                     HVPIIALTAHAMAGDRERCLQAGMDGYLIKPIRPATLLEAIQRLQVVAPARAAPAAAQ
                     VAEAGVPVLDREALLERVAGDHELLSEVTGMLMRDAPKLIADVRDALERRDRDAFGYS
                     AHTLRGMLRNFSAAAAEEAAARLQALDCARDGPRAEAACQALEREIQRLSGELAGMTK
                     EMIA"
     gene            11183..11311
                     /locus_tag="VI319_08910"
     CDS             11183..11311
                     /locus_tag="VI319_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864007.1"
                     /translation="MLIAARYYKCGLCTVTFRQSAPLAEAADVDRFSSGFLPPRGK"
     gene            complement(11497..11748)
                     /locus_tag="VI319_08915"
     CDS             complement(11497..11748)
                     /locus_tag="VI319_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864008.1"
                     /translation="MKEEKEIQHVIGFSVSDIEELPMGPARYIRIFRIRTLLGDETVL
                     TLRSESRLGLVLEAEREAPVIPKQKLRRRKDDRDHKPEH"
     gene            11966..13849
                     /locus_tag="VI319_08920"
     CDS             11966..13849
                     /locus_tag="VI319_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY6864009.1"
                     /translation="MNNNTNGQPSSLRALALAALGVVYGDIGTSPLYAFKEAFAGAHP
                     LALAEANVLAVLSMMFWSIALIVSLKYVGFVLRFDNGGEGGILALLARATRVTRDRPR
                     VAAAVAIVGIFGASLFYGDAIITPAISVVSAVEGMSVATPAFEHWIVPITIAILVGLF
                     AIQSRGTGSVGKLFGPVIVVWFLVIAGLGAHSIAQTPSVIGALDPRHALRFAWQFPGL
                     TFLVMGAVFLAITGAEALYADMGHFGRAPIRLAWFGLVFPALMINYFGQGALALRDPA
                     AMKNPFYLLAPQEWTLPLVLLATAATVIASQATIAGAFSVTQQATRLGYLPRVPTRHT
                     SESQPGQIYVPQLNWVMLVLVVLLVLEFKSSTALAAAYGIAVSGTMLLTTMLVGVVAW
                     FARGRLRVPFLALLAAIGLVELMFFAANTLKFADGGWFPLACGLAIFVALTTWKRAET
                     VLAAHESRRRLPLAELARLAGPEIPRVPGTAVYLSPDPDALPGVLLHNLKHNKVLHER
                     IVLLTLVDSDEPRVADRERTEVSVLEIGRAYQVKLHFGFMEEPDVPKGLKLLARHGLR
                     FDPMDTTFFLGRTALARAAKPGLFTWRRELFRWMQRNSPATAEYFKLMPERVVELGTR
                     VVL"
     gene            complement(13868..14167)
                     /locus_tag="VI319_08925"
     CDS             complement(13868..14167)
                     /locus_tag="VI319_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864010.1"
                     /translation="MDKPEPLFPAIAACPSCGKRTRLKWQELKPPARGVERVGILNCP
                     SCGGSFLRTVGTADGIAALAQALREEHVRHHQHDHGEPRILGRTERWAYIEVPHH"
     gene            14185..15987
                     /locus_tag="VI319_08930"
     CDS             14185..15987
                     /locus_tag="VI319_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY6864011.1"
                     /translation="MRSATIGHVRLRSSLVTLVVATVVPLVLFAALVTVFLVRYERDN
                     FVQAMNDRNRAFLSAVDAELKGQITSLHALAASRGLAVDDLRAFHDDASAVLRTQTDW
                     LNVVLTAPGGRQLVNARAPWGSRLRATPVQPDTLARVADTLAPVVGNVAAPGAIVRLP
                     GIPVRVPILRKGALVYVLTAIVDPDVFQRLIVSQQLPSGWVSGLVDASGKFVARVPPQ
                     PIGSPASAQYLARQRSQREGFYRGTTVEGIDTYTAYGSSDFSGWSVGLAIPASAVLGG
                     AVHAAWMMAAGVIVFLAIAIGIALWLARRIAGPIEALASAAPALGTAAARVRTRTAVK
                     ELQQLAWALDGASHAIRERGELLVRERDALRSADRAKQEFLAMLSHELRNPLAAIANS
                     AYLLKSAGTDREMLATAQRVIERQTQHMKKLVDELIDVSGIAMGKAKLELEPLDVAEL
                     VSRLVEGWRHRLGNHDVRLSAAPVWVKADRRRLEQVVSNLLDNAAKYTPAHKAITVHV
                     GGEGGTARIVVQDEGIGIAPADLERVFDLFAQADQGLSRSGGGIGVGLAVVKRVVEMH
                     GGTVVAESAGPGSGSRFTVNLPAVEAPDAERAAA"
     gene            16038..>16711
                     /locus_tag="VI319_08935"
     CDS             16038..>16711
                     /locus_tag="VI319_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864012.1"
                     /translation="MKHTARLVISLAAAALAAVSFPCSAQGYPAKPVLVVMPLQAGSA
                     VDVMIRIVAQRMSENLGQQIVIENQPGAAGSIGAERVKRAAPDGYTVGVLNDSILTMI
                     PNIRHVGYDPTKDFEPVGVVAAITWVMVVNNDVPAKTVREFIDYAKARPGKIDYSSGG
                     NGSPQHVAMEAFKAATGLDLVHIPYKGATQAAIDIMSGRVQAHFGAVSIVQPYVKDGR
                     MRALGVP"
BASE COUNT         2500 a         5851 c         5841 g         2519 t
ORIGIN      
        1 gccggcgcca ggcttctgtc cgtaccgctc gctgctcgcg accggtgcgg cgctcttcgg
       61 ccgtcccgag ttcaaggcca aagcgagcgc gctcgacgac aagacgcgct ggctcctcgg
      121 cgacgcggac gagcgctatg ccgccgtcga cgactcgcgc gcgcgcctgc cggtgcgcag
      181 ggaattcccc gagggaggct accgcgtgct cgggaccgac ttcgagaccg cccgcgagat
      241 ccggctcgtg gtcgactgcg gcccgctcgg ctacacgcgc atcgccgcgc acggccacgc
      301 cgacgcgctc gccttcacgc tttcggtcgg gggcctcgag ttcctggtcg accccggcac
      361 ctacgcctat cacacgcaag gccggtggcg tcagtatttc cgcggcacct cggcgcacaa
      421 cacgctgcgc gtcgacggcg agtcgcagtc gcagccgggc ggcagcttcc tgtggctggc
      481 gaaggccgag gcccgatgcg acgcctggga cacgtccgac gagcgcgacg tcttcgaggg
      541 ccggcacgac ggctaccggc gccttccgga ccccgtcgtg caccgccgcc gcgtcgtcct
      601 cgagaagcgc gagcgccgca tcctggtcga cgacggggtc gaggcgcgcg gcgcgcaccg
      661 cgtcgaggcc ttcttccact gccacgaggc ctgcgcggtc gagcccgtgc cgggcgggtt
      721 cgccctgagg cgcgacggca ggacggtgat gctgctgttt cccgacgcgg gcgtctcgga
      781 tacgagcgtg ctgcgcgcga gcgtcgatcc gatcggcggc tgggtgtcgc ggcgcttcga
      841 cgacaaggtt cccgcgacga cgatcgtgcg ccgggcgacg accgccggga acgctgtgct
      901 gcgcacgctc atcgtctgct gaagctctgt tgcctgccga cgacgcttcg ctagtccatc
      961 gggcgcaacc cgggcggact accgcgcatg accggcgcgg acagcaaccc tgtcagcgcg
     1021 cgcgctcaat cttccttctt ctgcaggaga ggcatccgag atgaaaatca gcgtcatggg
     1081 gctgggctat gtgggggccg tgtcggccgc ctgcctggcg aagcagggcc acgaggtgat
     1141 cggggtcgac ccgcagcggg cgaaggtcga cctgatcaac gagggcagga cccccgtcat
     1201 cgagcgcgac ctcggggaga tcatcgccga ggcggtcggc gacgggcgcc tgcgcgcgac
     1261 ctccgacgtc gtggccgccg tgcagcacac cgacctcgcg ttcgtgtgcg tcggcacgcc
     1321 gagcctgccg aatggcggca tcgacctccg gtacatgcgc cgggtgtgcg aggagatcgg
     1381 acaggcgctg cgcggccatg ccggcgcgcc gaccatcgtc atccgcagca ccatgctgcc
     1441 agggacgatg cgcgacgtcg tcatcccgac cctcgagtcg ggctcgggca agcgcgcggg
     1501 cgcggagttc ggcgtgtgca tcaaccccga gttcctgcgc gagagcaccg cggtgcacga
     1561 ctatttcaac ccgccgaaaa ccgtcatcgg cgaggtcaac cgcgcgagcg gcgacgtgct
     1621 cgcgagcctg tacgcgggcc tgtcggcgcc gatgatccgg acggatgtcg agaccgccga
     1681 gatggtgaag tacgccgaca actgctggca tgcggtcaag gtcgggttcg cgaacgagat
     1741 cggcaacatc tgcaaggcgg tcgacgtcga cagccaccgg gtcatggaga ttttctgcca
     1801 ggacacgaag ctcaacctct cgtcctatta cctcaagccc gggttcgcgt tcggcggctc
     1861 gtgcctgccc aaggacctgc gcgccctgct gcacaaggcc aagtcgctcg acgtggacgt
     1921 gcccatcctg gcgtcggtgc tgccgagcaa cgagctgcag atcgaccgcg ccgtgcgcgc
     1981 cgtgatcgac gcggcggcga aggcgcgcag ccgcaagatc ggcgtcctgg gattcagctt
     2041 caaggcgggc accgacgacc tgcgcgagag cccggtggtc gagctgaccg agcggctgat
     2101 cggcaagggc tacgagctgc gcatctacga ccgcaacgtc agcctcgcca gcatccacgg
     2161 cgcgaaccgc gactacatcc tcaatcacat cccgcacatc tcgcgcctca tggtcggctc
     2221 gatcggcgag gtgctggacc acggcgcgac gatcgtcatc ggcaatgccg atcccgactt
     2281 ccgcggcgtg cccaagctgc tcggcgacgg gcaggccgtg atcgacctgg tgcgcgtcgc
     2341 cgactcgcgc agcgtgagcg gggtgtacga aggcatctgc tggtgagcgc gcgatgaccg
     2401 ccgtctatac gccggtcccc ggcgccggca acgacgacgc cccgctcccc aaggcgctgc
     2461 gcgcgccgat cgcgcgcggg gagctgcgcg acgaggcgca actgcgcgag cactacctga
     2521 tcgagcgcga gctggccgac cggctgcgcc gcgcggcgcc cgacgagcgc cggcgcctgt
     2581 atgcgcaggt ctacgacgag ctgtaccgcc gcgtgccgca ccatccgatg aaccggccgt
     2641 ccgggcaccc gcgcgcggcc ggcgtcgagc gcgacctcgt gttcctgcgg cgcttcctgc
     2701 gcccgacgac cgcgttcctg gagatcgggg ccggcgactg cgcgctcgcg tgccgcgtcg
     2761 cggccgtcgt gcgccgcgtc gtcgccgtcg acgtctccga ggagatcatg cggctcgcgc
     2821 cttccgcgat gaacctcgag cgcgcgctct ccgacggcac cagcatcccg gtgcgcgcgg
     2881 gcagcatcga cgtcgcgttc agcgaccagc tcatggagca cctgcacccc gacgacgccg
     2941 cggagcagct gcgcaacatc caccgcgcgc tcgcccccgg cggcgtgtac ctttgcatca
     3001 cgcctaaccg cctctacggg ccgcgcgaca tctcgggcta tttcgacgac gtcgcgaccg
     3061 gcttccacct gcgcgagtac acgggcgggg agctggcgcc gctgttccgc tcggtcggct
     3121 tctcgcgcct gcgcttctac gccggcgcgc gcggctggta cctgccggtg cccgcggcgc
     3181 cggtgcacgc ggccgagacc gcgctcgaga tgctccccgc gcctgcgcgc cggcgcgtcg
     3241 ccggcgtcgc cccagtgcgc gcgctgctcg ggctgcgcgt ggccgcgatc aagtgagtcc
     3301 gggcacgggg gtggcgaagc gcgtcctgat cctcgtcgag aacctgccca gcccgttcga
     3361 ccggcgcgtg tggcaggagg cgacggcgct gcgcgatgcg ggatggacgg tttcgatcgt
     3421 ctgtcccacg ggacgcggct acgagcaacg cttcgagcgc ctcgacggca tcgacatcca
     3481 ccgctacccg ctgccggtcg aggcctcggg cgcgctcggc tacgcgctcg agtacggcgc
     3541 ggcgctgttc tggtcgttcg tcctgtcgtg gaaggtcttc ttcggacgcg gcttcgacgt
     3601 cgtccacgcc tgcaacccgc cggacctgtt cttcctcgtc ggcgccgtgt tcaagctctt
     3661 cggccgcaag ttcgtgttcg atcatcacga cctcaacccc gagctgtacg aggcgaagtt
     3721 cgggcgccgg gatctcttct accgcgcgat gctcgcgctc gagcgctgga ccttccgcac
     3781 cgcggacgtc tcgatcgcga ccaacgagtc gtaccgccgc atcgcgatcg agcgcggcgg
     3841 catggcgccc gggcgcgtgt tcgtggtcag gagcggcccg agcctggagc gcatgaagat
     3901 cgtgccgccg gtggagacgc tcaaatgcgg gcgccggtac ctcgtcggct acgtcggggt
     3961 gatggggcgt caggaaggga tcgacctgct gctgcgggcc gcgcgcgaga tcgtccacga
     4021 gcgaggccgc ggcgacgtgc atttcgggct cgtcggcggc ggcacgtcgc tcgaggagat
     4081 gaaggcgctc gcgcgcgagc tgcgcgtgtc cgaccacgtc acgttcaccg gccgcgtccc
     4141 cgacgccgcg ctgctcgcga tgctcaatac cgccgacgtc tgcgtcaacc ccgacgtcgc
     4201 gaacgagatg aacgacaagt ccacgatgaa caagatcatg gagtacatgt cgctcggcaa
     4261 gccgatcgtg cagttcgacc tcaccgaagg ccggttctcg gcgcaggagg cgtcgctgta
     4321 cgcacgccgc aacgacccgg cggacctggc ggcgaagatc cgcgagctgc tcgacgaccc
     4381 ggagcggcgc gcgcgcatgg gcgccttcgg ccggcgccgc gtcgagcagg agctcgagtg
     4441 gcgccacgag gtcccgaagc tgctcgccgc gtacgaggcg ctgcggtcgc cgtcgcgcgc
     4501 ggcgccggcg gcgcagcgct actgatcgtc gtcgagacgg atcccgacca ccgcgtactc
     4561 gggcgcgccg ctcagaaaag tcttcagtcg cagcaccttg ttcctggtcc cgcaatgcgc
     4621 gcacacgacg tacggcgcct tgtcggcgtc gtgaacctcg acgagccgcg acggcttctt
     4681 gcagcccaga caaatgaagc tgtccctggg cacatctcct cccgctggat ccgagcggaa
     4741 tatatacgcc ggataatctg tacgggctag tgtctcggaa aggcgacatc gccgattcgc
     4801 tacatgctgt agttgaagct cgacggcgga atcgcgccgc ggcgataccg gcggggagcc
     4861 tcgcgctcta tgatttcgct ggtgttccgg tgttcgaaat gaaggtcctg atcgctgacg
     4921 actcgcagat cgtaagagag aggctggctt atctgctcgg cgacgtcgaa ggcgtcgaga
     4981 tcgtcgggca ggcggaggat gcggtggagg gaagcaatct cgccgaggcg ttgaagcccg
     5041 acgtcgcgat actggacgtg cgcatgccgc gcggcagcgg gctggacgtg ctgcgctcca
     5101 tcaagagcca ccacccgtcc gcgacggtca tcatcctcac caatttcgtc gaccccgagg
     5161 cgcgccagct gtgcctcgcc cgcggcgccg actacttctt cgacaagtcg atcgagttcg
     5221 acaaggcggt cgacgtgttg cgcgggctcg cgcagccgga gaccccgtga gcgacatgaa
     5281 gttcgaggac ggcctcgacc gccgctccat cctcgccgcg ctgcgcgcgt accgcaaggg
     5341 cgacttctcg gcgcgcctgc cgctcgacct cgtcggcgtc gacggcgaga tcgcgcaggt
     5401 cttcaacgac ctggtcgaga ccagcgcagc gtgcgcggcc gaggtcgcgc gcgtgcgcga
     5461 cgaggtgggc gggcagggac ggctcgcgca gcgcgcgggc gtgcccgccg cggcgggcgg
     5521 ctgggcgtcg ctgctcgagg gggtgaacgc gctgctcgac gactcggggc gcccgctcgc
     5581 cgagatcgcg cgcgtggtgc aggccgtgac gcacggggac ctctcgcgcg ccatggcgct
     5641 cgagatcgac ggccgcccgc tgcgcggcga gctcctgcgc atcgcgcagg cggtgaacgc
     5701 catggtccgg cacctcgccg cgcaggattg gctgaagtcg aacctcgcgc gcttcacgcg
     5761 catgctgcag gggcacggcg acctcgtcac cgtgtcgcgc ctggtgctct cggagctggc
     5821 gccgctcgtg agcgtccagc gcgccgtgct ctacgggcgc gcgaagcgcg ccgccgagcc
     5881 gcggctcgac ctgctcgcga cctacgcgac ctcgtccgac caggcggtgc cgccgtcgct
     5941 gcgcatcggc gagggcctgg tgggccagtg cgcgtacgag aagaagcgcc tgctgctcga
     6001 ggaggtgccc gcggactacc cgcgcgcgag ctcggggctg ggatcggtga ggccgctcaa
     6061 catcgtcatc gtgccggtcc tgttcgaggg cgagatcgag gccgtgatcg agctcgcgtc
     6121 cttcaggaag ttcggcgaca cgcagctcgc ctttctcgac cagcttgccg agagcatcgg
     6181 catcgtattc aacacgatcg aggcgaatat gcgcacggtc gagctgctcg agcgctcgca
     6241 ggcgctgacg cgcgagctgc aggggcagca ggacgggctg atcaagacca acgaccgcct
     6301 ggagcgccag gccgatgctc tgcgcaagtc ggagagcctg ctgctcaagc agcaggacga
     6361 gctgcggcgc tcgaacgagc agctcgccga gcgcgcgaag gcgctctcgg agcagatgaa
     6421 gcaggtcgag ctgcgcaaca aggaggtcga gcaggcgcgc gccgccctcg aggagaaggc
     6481 cgagcagctg gcgctctcgt cgcgctacaa gtccgagttc ctcgccaaca tgtcgcacga
     6541 gctgcgcacg ccgctcaaca gcttgctcat cctcgccaag atcttcgccg acaacgccgc
     6601 gagcaacctt acccccaagc aggtcgagta cgcgcagacc atccacgccg cgggcaccga
     6661 cctgctctcg atcatcaacg acatcctgga cctcgccaag atcgagtcgg gcaccgtgac
     6721 cctggagatc gggcacgagc cgttctcgca attgcgcgat tacgtcgagc gcacgttccg
     6781 gcaggtcgcg atcggcaagc gcctcgactt cgccgtcact ctcgacccgg cgctgcccga
     6841 ggccatcgag accgactcga agcgcctgca gcaggtgctg aagaacctgc tctcgaacgc
     6901 gatcaagttc accgagcgcg ggcgcgtgtc gctgcacatc gcgccggccc gctcgggctg
     6961 gctgccgggc aacgcccgcc tcgacggcgc gagccacgtg gtgtcgttcg cggtcaccga
     7021 taccggcatc ggcatcgccg agagcaagca gaagatcatc ttcgaggcgt tccgccaggc
     7081 cgacggctcg accgggcggc gctacggggg caccgggctc ggcctctcta tctcgcgcga
     7141 gctcgcgcgc ctgctcggcg gcgagatccg cgtgtcgagc gcgcccggga agggcagcac
     7201 cttcgtggtc tacctgccgg tcatgcacaa ggcgaccgag ggagagccgg cgccggcggc
     7261 cgcgcgcgcg ccggccttca gcgcggcgta cgcgtcgcgc tcggcgccgc gtgccgctcc
     7321 gcgcccgcag ccgcaggtgc ccgccgcggt catgcgcgag gtgaaggagg agctcgccgg
     7381 ccgcaaggtc ctcgtggtcg acgacgacat ccgcaacatc ttcgcgatga ccgcttcgct
     7441 cgagcagctc gggatgacgg tggcgcacgc cgagagcggc aaggagggca tcgaggtcct
     7501 gaagagcgag cccggcatcg acgtcgtgct gatggacatc atgatgcccg acctcgacgg
     7561 ctacgacacg atccgcatca tccgcgggct cgacgggctg cgcgacgtcc cgatcatcgc
     7621 cgtcacggcc aaggcgatga aggaggatcg cgagcggtgc ctgcaggccg gcgccaacga
     7681 ctacatctcg aagcccgtga acatggagca gctctcgctc atgctgcgcg cctcgctcgc
     7741 ccgctcgctc ggctgacggc atggccgtgc gcacctccaa ggccaacatc ctcgtcgtcg
     7801 acgacgagct gcacagcctg atcgcgctgc agcacctgct gtcgggaccc gaccgcaacg
     7861 tcgtgccggc gcgctcgggg cgcgaggcgc tgcgccacgt tctcaagaac gacttcgccg
     7921 tcatcctgct cgacgtgcgc atgcccgaca tggacggctt cgagacggcg gggctgatcc
     7981 ggcagctgcg gcgctgccgc cacacgccga tcatgttcct caccgcggcc gtcgaggaca
     8041 cgcagtcgat gttccgcggc tacgaggtcg gcggcgccga ctacatcctc aagcccgtgg
     8101 atccggcggt gctgacctcg aaggtcgccg tgttcgtcga cctgtacggc aagagcgccg
     8161 agctgacgac gcagatccac aagcgaaaag aggcggagcg cgagctctat cggaccaacg
     8221 aggacctcga gaccaagatc cgcgagcgta ccgcgagcct gatcgcagcg aacgacctgc
     8281 tgcagaagga gatcgcgcgg cgagagcgca tcgaggagcg cctgcgccgc agcgaggccg
     8341 aggcgcggcg cctgtcgctg gtcgcgagcc tgacggagaa cgcggtcctg atcaaggatg
     8401 ccgacgaccg cgtcgagtgg gtgaacgaga gcttcacgcg gatgttcggg tacccgctcg
     8461 acgaggtgcg cggccgccgc accgacgagg tgttcccccg cgaggccggg gacctcggcg
     8521 tcgcggccgg cacggacgtc gagggaggct accgcgccga gagggtccgc cgcagccgcg
     8581 gcggccgcaa gtactggctg gccgtggagt gccggccgat cctggacgac ggcggcgcgc
     8641 cggccgggtt catcgagatc gcgaccgaca tcacggagcg caagctctcg gaggagtcgc
     8701 tgcgcgagag cgaggcgcgc aagacggcga tcctggagtc ggcgctcgat tgcatcgtga
     8761 cgatggacca caagtgccgg atcctcgagt tcaacccggc cgccgaaaag gccttccgct
     8821 gcgcgcgcgc cgaggcggtg ggccgctatg cgccggggct gctgctgccg gcggcgacac
     8881 gcaaggcgca gctgcgcctg ctgggcacgc tgctcgcgcc gggcgatccg gcagaccgcg
     8941 cccgccgcgt cgagatcacg gcgcggcgct gcgacggcag cgagttccca gccgagctga
     9001 ccatcgcgat ggcgaccctc ggcggccggc cgttcttcgc cgcctacgtg cgcgacatca
     9061 ccgcgcgcaa ggaggccgag gccgacctgc acaaggcgaa gcaggccgcc gaggcggcga
     9121 acctggcgaa gtctcagttc ctcgccaaca tgagccacga gatccgcacg ccgatgaacg
     9181 ccatcatcgg catgaccgag ctcgcgctgc agacctggct gaacggcgag cagcgcgagt
     9241 atctcgggct ggtgaaggac tcgggcgaga cgctgatgaa gctcatcaac gagatcctcg
     9301 acttctccaa gatcgaggcg gggcgcctcg aggtcgagac catcccgttc tcgctgcgcg
     9361 agagcgtcgg cgacacgatg aagacgctcg gcttccaggc gcacaagaag aagggcctgg
     9421 agctcgcgta cgacatcgcg cccgaggtgc ccgacgcggt ggtcggcgat cccgtgcggc
     9481 tgcgccagat cgtcatcaac ctggtcgaca acgcgatcaa gttcaccgag cgcggcgaga
     9541 tcgtgatgcg cgtgtcgtgc gagggcatcg acgccggcgg cgtcgtgtgc cgcttcgccg
     9601 tgcgcgacac gggcgtcggc atcgcgcgcg agaagcaggc cgcgatcttc gcgccgttcc
     9661 tccaggccga cgcctcgacg acgcgcatct tcggcgggac cggcctcggc ctcaccatct
     9721 cggcgcggct ggtcgagatg atgggcggcg agatctgggt cgagagcgag ccgggcgcgg
     9781 gcagcatctt ccatttcacg gtgcgcttcg ggctgcagcg ggccgcggag attccgttcg
     9841 aaaactcggc ggccgacttc gccggcgccc gcgcgctcgt cgccgaggac catccggtga
     9901 gccgcggctt catcgtgtcg acgctgcggc aatggaacat cgaggtggtc gaggccgcga
     9961 gcgccgccga agcgatggag tcgatcgccc gcagcgccgg cgcgcccttc cacctcgcgc
    10021 tgatcgacga cgcgctgccg ggcggggacg cggcgggcct gctcgcgcgc ctgcgccaca
    10081 gtggcggcgc cgcgtgcccc gccgtgatcg tgctcgcgtc ggcgttgcgc cgcgaaggcg
    10141 ccgaggccgg cgccgcgctg atcgccaagc cggtcaagcc ctcggagctg ctcgccgcgg
    10201 tgcgcgcggc gctcatcggc gacgccggcg cgcaggccgc ctcggcgccg ccggcggcgg
    10261 cgtccgcgcg cgagcgcctg cgcgggctcc tggtcgagga caatcccgtc aaccacaagc
    10321 tcgccgtgca cgtgctggag aaggacggcc acgacgtcgt cgcggcggag aacggcgagg
    10381 tcgcggtctc ggttctggat tcgtcgacct tcgacctggt cctgatggac gtacagatgc
    10441 cgaagatgga cggcatcgag gcgacgcggg cgatacgcga gaaggagcgc tcgagcggcg
    10501 cgcacgttcc gatcatcgcg ctcaccgcgc acgccatggc cggggaccgc gagcgctgcc
    10561 tgcaggccgg gatggacggc tacctgatca agccgatccg gcccgcgacg ctgctcgagg
    10621 cgatccagcg tctccaagtc gtcgcgcccg cgcgcgcggc gccggccgcc gcgcaggtcg
    10681 ccgaagccgg tgtcccggta ctcgaccgcg aggcgctgct cgagcgcgtc gcgggcgacc
    10741 atgagcttct ctccgaggtg accgggatgc tgatgcgcga cgcgcccaag ctcatcgccg
    10801 acgtgcgcga tgcgttggag cgccgcgacc gggacgcctt cggctactcc gcgcacacgc
    10861 tgcgcgggat gctgcgcaac ttctcagcgg ccgcggccga agaggccgcc gcgcggctgc
    10921 aggcgctgga ctgcgcgcgc gacggcccgc gcgccgaggc cgcgtgccag gcgctcgagc
    10981 gcgagatcca gcggctgagc ggcgagctcg cgggcatgac gaaggaaatg atcgcctgag
    11041 cgggtcccgc cagtagtccg tcggcaggcg gtcgccggac acgaaagcga caggcttgcc
    11101 gccgtagact tggccgatgt cccgagacac gccaccgcct tgcccggcgt gcggatcgcg
    11161 cgacgtcgag cgcgttcctc tgatgctcat cgcggcccgc tactacaagt gcggcctgtg
    11221 cactgtcacg ttccgccaga gtgcgccgct cgccgaagcg gcggacgtcg atcgcttcag
    11281 cagcggcttc ctgccgccgc gcgggaaata aaaaagcccc ggactaaagg gataggccgg
    11341 ggctgaaaat gcctttagaa ggcatcccgc tcagggagga gaagcgggag atggccgaag
    11401 ccacctgtat gttcagaacg cagcgctgcg gaaaggttcc accggagcgc ggtccggtcg
    11461 gatcatcggc gagcgagcga tgctcgcgcc attcattcaa tgctcgggct tgtggtcgcg
    11521 gtcgtctttt ctgcgccgca gcttctgctt cgggatgacg ggggcttcgc gctccgcttc
    11581 gagcaccagc ccgaggcggc tctcgcttcg cagggtcagg accgtctcgt caccgagaag
    11641 cgtgcggatt ctgaagatac gtatatagcg cgcaggaccc atcggcagct cctcgatgtc
    11701 ggacaccgaa aagccgataa catgctgaat ttccttctct tccttcaatc tgcctccgcg
    11761 cgccgttctc cccagcaaga gcggcggatt ttttgttgtg cgtcccctgc gccgctgttg
    11821 gcggcgatac cttaggagtg ccaaatccgt gacttagttc attcggatca gggcctagcg
    11881 tgtgaatttg taagcatagt gctgtgttgc attgcaacat tccgaggcgt agagtggctt
    11941 ccctcgaggt cccggaaggc ttcccatgaa caacaacacc aacggccaac cctcgtccct
    12001 gcgcgcgctc gcgctcgccg cgctcggcgt cgtgtacggc gacatcggca ccagcccgct
    12061 gtacgcgttc aaggaagcct tcgccggagc gcatccgctc gctctggccg aggccaacgt
    12121 gctcgccgtg ctgtcgatga tgttctggtc gatcgcgctc atcgtgtccc tcaagtacgt
    12181 cggcttcgtg ctgcgcttcg acaacggcgg cgagggtggc atcctggcgc tgctcgcgcg
    12241 cgcgacgcgc gtgacccgcg atcgtccccg cgtggcggcc gcggtggcga tagtcgggat
    12301 cttcggcgcg tcgctcttct acggggacgc gatcatcacg ccggcgatct cggtcgtgtc
    12361 ggcggtcgaa ggcatgtcgg tcgcgacgcc cgccttcgag cactggatcg tgccgatcac
    12421 gatcgcgatc ctggtcgggc tgttcgcgat ccagagccgc ggcacgggat cggtcggcaa
    12481 gctcttcggg ccggtcatcg tggtctggtt cctcgtgatc gcgggcctcg gcgcgcactc
    12541 gatcgcccag acgccgtcgg tcatcggggc gctcgatcct cgtcacgcgc tgcggttcgc
    12601 gtggcagttc cccggtctca cgttcctggt gatgggcgcg gtgttcctcg cgatcaccgg
    12661 cgccgaggcg ctctacgccg acatgggaca cttcggccgc gcgccgatcc ggctggcgtg
    12721 gttcggcctc gtgttccccg cgctgatgat caattacttc ggccagggcg cgctcgcgct
    12781 gcgcgatccc gcggcgatga agaacccctt ctacctgctc gcgccccagg agtggacgct
    12841 gccgctcgtg ctgctcgcga ccgccgcgac cgtgatcgca tcgcaggcga cgatcgcggg
    12901 cgcgttctcc gtcacccagc aggcgacgcg cctcggctat ctgccgcgcg tgccgacccg
    12961 ccacacctcc gagagccagc ccgggcagat ctacgtgccg cagctgaact gggtcatgct
    13021 cgtgctggtg gtgctgctcg tgctcgagtt caagtcgtcg acggcgctcg ccgccgccta
    13081 cggcatcgcc gtgtcgggca cgatgctcct cacgacgatg ctggtcggcg tcgtcgcctg
    13141 gttcgcgcgc gggcggctcc gcgttccatt cctcgcgctg ctcgcggcga tcgggctcgt
    13201 ggagctgatg ttcttcgccg ccaacacgct gaagttcgcc gacggcggct ggttcccgct
    13261 cgcgtgcggg ctcgcgatct tcgtcgcgct cacgacctgg aagcgcgccg agaccgtgct
    13321 cgccgcgcac gagtcgcgcc ggcgcctgcc gctcgcggag ctcgcgaggc tcgcgggccc
    13381 cgagatcccg cgcgtcccgg gcaccgccgt ctatctttcg ccggaccccg acgcgctgcc
    13441 cggggtgctg ctgcacaatc tgaagcacaa caaggtgctc cacgagcgca tcgtcctcct
    13501 gacgctcgtc gattccgacg agccgcgcgt cgcggaccgc gagcgcaccg aggtgagcgt
    13561 gctggagatc gggcgagcgt accaggtgaa gctgcacttc ggcttcatgg aggagcccga
    13621 cgtcccgaag gggctgaagc tgctcgcgcg gcacgggctg cgattcgacc cgatggatac
    13681 caccttcttc ctcgggagga ccgcgctcgc gcgcgcggcg aaaccgggac tgttcacgtg
    13741 gcggcgcgag ctgttccgct ggatgcagcg caacagcccg gcgaccgcag agtacttcaa
    13801 gctcatgccc gagcgggtcg tcgagctggg gacgcgcgtc gtgctctagc tcgcggcgat
    13861 gcgccgccta gtggtgcggc acctcgatgt acgcccagcg ctcggtgcgt ccgaggatcc
    13921 tgggctcgcc gtggtcgtgc tggtgatggc gcacgtgctc ctcgcgcagc gcctgcgcga
    13981 gcgcggcgat gccgtcggcg gtcccgacgg tgcgcaggaa cgatccgccg cacgaagggc
    14041 agttcagaat gccgacacgc tcgacacctc gcgcgggtgg tttgagctcc tgccatttca
    14101 ggcgcgtgcg cttgccgcag ctcgggcagg cggcgatggc ggggaagagc ggctcgggct
    14161 tgtccatcgc cgaagtataa ggccgtgcgc tcggctacaa tcggccacgt gcgcctgcgt
    14221 tccagcctcg tcaccctcgt cgtcgccacc gtcgtgccgc tcgtgctgtt cgcggcactc
    14281 gtgaccgtct tcctcgtgcg ctacgagcgc gacaacttcg tccaggcgat gaacgatcgc
    14341 aaccgggcgt tcctgagcgc ggtcgacgcc gagctgaaag gacagatcac gtcgctgcac
    14401 gcgctcgccg cgtcgcgcgg cctcgcggtc gacgacctgc gcgcgttcca cgacgacgcc
    14461 tccgcggtgc tgagaaccca gaccgactgg ctgaacgtcg tgctcaccgc gccgggaggc
    14521 cgccagctcg tgaacgcccg cgcgccctgg ggcagccggc tgcgcgcgac gcccgtgcag
    14581 cccgacacgc tcgcccgggt cgcggacacg ctcgcgcccg tggtcgggaa cgtcgcggcg
    14641 cccggcgcca tcgtgaggct cccgggtatt ccggtccggg ttcccatcct gcgcaaaggc
    14701 gcgctcgtct acgtgctcac tgccatcgtc gatccggatg tgttccagcg gctgatcgtg
    14761 agccagcagc tgccgtccgg gtgggtcagc gggctggtcg acgcgagcgg caaattcgtc
    14821 gcccgcgtcc cgccacagcc catcggcagc ccggcgagcg cgcaatacct cgcgcggcag
    14881 cgctcgcagc gcgaagggtt ctatcgcggc acgaccgtcg agggcatcga cacgtacacg
    14941 gcgtacggca gctcggattt ctcggggtgg agcgtcggac tcgcgattcc cgcgtcggcc
    15001 gtgctcggcg gcgccgtgca cgccgcctgg atgatggcgg ccggcgtgat cgtttttctc
    15061 gcgatcgcga tcggcatcgc gctctggctc gcccgacgga tcgccgggcc gatcgaggcg
    15121 ctcgcgtcgg cggcgccggc gcttggtacc gcggctgcgc gggtccggac gcgtaccgcg
    15181 gtcaaggagc tgcagcagct cgcctgggcc ctcgacgggg cgtcgcacgc gatccgcgag
    15241 cgcggcgagc tgctcgtgcg cgaacgcgac gcgctgcgct cggccgaccg tgccaagcag
    15301 gaatttctgg cgatgctttc ccacgagctg cgcaacccgc tcgccgcgat cgcgaactcc
    15361 gcttatctgc tcaagagcgc cgggaccgac cgcgagatgc tggcgacggc gcagcgcgtc
    15421 atcgagcgcc agacgcagca catgaagaag ctggtggacg agctgatcga cgtgagcggc
    15481 atcgccatgg gcaaggcgaa gctcgagctg gagccgctcg acgtcgccga gctcgtgtcg
    15541 cgcctggtcg aaggctggcg gcaccggctg gggaaccacg acgtccgcct ttcggccgcg
    15601 ccggtgtggg tcaaggccga ccggcgccgg ctcgagcagg tcgtgtccaa cctgctcgac
    15661 aacgcggcga agtacacgcc cgcgcacaag gcgatcacgg tccatgtcgg cggggagggt
    15721 ggcactgcgc gtatcgtcgt ccaggacgag ggcatcggca tcgcgcccgc cgacctcgag
    15781 cgcgtgttcg accttttcgc gcaggccgac caggggcttt cgcgctcggg cggcggcatc
    15841 ggcgtcggcc tcgcggtcgt gaagcgcgtc gtcgagatgc acggcggcac ggtggtcgcc
    15901 gagagcgccg gaccgggctc gggctcccgc ttcacggtca acctgcccgc ggtcgaggcg
    15961 ccggacgcgg agcgcgccgc cgcctgagcc ggcgcgtcgg tgctattctc tcgcgtcgct
    16021 cgagaggagg acgcaaaatg aagcacaccg ccaggctcgt gatctcgctc gccgccgcgg
    16081 cgctcgccgc ggtctcgttt ccgtgctccg cgcagggcta cccggcgaag ccggtgctcg
    16141 tcgtgatgcc gctgcaggcg ggcagcgccg tcgacgtcat gatccgcatc gtcgcccagc
    16201 gcatgagcga gaacctcggc cagcagatcg tgatcgagaa ccagcccggc gctgcgggct
    16261 ccatcggagc cgagcgcgtg aagcgcgccg cgcccgacgg ctacaccgtc ggcgtgctga
    16321 acgattcgat cctcaccatg attccgaaca tccggcacgt cggctacgac ccgacgaaag
    16381 acttcgaacc ggtcggcgtg gtggcggcga tcacctgggt catggtggtc aacaacgacg
    16441 tgccggcgaa gacggtgcgc gagttcatcg actacgcgaa ggcccggccg gggaagatcg
    16501 actattcctc cggcggcaac ggcagcccgc agcacgtcgc gatggaggca ttcaaggcgg
    16561 ccaccggcct cgatctcgtg cacatcccgt acaagggcgc gacgcaggcc gcgatcgaca
    16621 tcatgtccgg acgcgtgcag gcgcacttcg gcgcagtgtc gatcgtgcag ccgtacgtga
    16681 aggacggccg catgcgcgcg ctgggcgtgc c
//
LOCUS       DATDMW010000208         3212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1558433, whole genome shotgun sequence.
ACCESSION   DATDMW010000208 DATDMW010000000
VERSION     DATDMW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558433"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /gene="aceE"
                     /locus_tag="VI319_08940"
     CDS             complement(<1..1712)
                     /gene="aceE"
                     /locus_tag="VI319_08940"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY6864013.1"
                     /translation="MDRIPDIDPQETREWLEALDGVLATQGPDRAHFLIEQLIDKARR
                     SGAYLPFSANTAYINTISPERQARIPGDQNIEHRIRSYTRWNAMAMVVRANKHTNVGG
                     HIASYASAATLYDVGFNHFWHGMSDKHAGDLVFFQGHSSPGVYSRAFLLGRLTESQLD
                     NFRQEVDGKGIASYPHPWLMPDFWQFPTVSMGLGPLMAIYQARFMKYMHDRGLANTEG
                     RKVWCFCGDGEMDEPESMGAIGMASRENLDNLIFVINCNLQRLDGPVRGNGKIIQELE
                     SEFRGAGWNVIKLVWGSRWDALLTRDRKGILMRRMMEAVDGEYQTFKAKDGAYVREYF
                     FNSPELKELVADYSDKDIWDLNRGGHDPHKVYGAYHAAVNHVGQPTVILAKTIKGYGM
                     GIGFGEAQNVTHQQKKMNVEAIRRFRDRFAIPVSDEKLEEVPYVKFAEGSPELEYMRA
                     RRMELGGYLPARRRKAEPLVIPPLSAFERFLKSTDEREISSTMAFVQILQMLLRDKNL
                     AKHIVPIVPDESRTFGMEGLFRQLAIWNQLGQLYTPEDKDQLMFYKEDKHGQILQEGI
                     NEAGA"
     gene            complement(1741..2937)
                     /locus_tag="VI319_08945"
     CDS             complement(1741..2937)
                     /locus_tag="VI319_08945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6864014.1"
                     /translation="MTSARTAAIALAATLAIQIYTAFAAAAAAVLAPEIALAFDVEAR
                     WIGAFIGLVYAGGMIASLASGGFIERLGSIRVSQACVVLCALGVAGIAFAPAHAIALV
                     AAFGIVIGLGYGPITPASSQLLQRTAPPARMALTFSIKQTGVPAGAALAGALLPALAL
                     ATGWRGAFAIAALAGLAIVAVAQPIRAGLDADRNPKGRVSVAGVFAPLRLLRHSRALR
                     DLAFVAFAYSATQVCLTSFLVVYLTETLGYSLVAAGLALTIATIGGVAGRVAWGYVAD
                     RWLAPRRVLATIGFCAGACGVLMASATTAWSPLAIGVLAALFGATAIGWNGVQLSEVA
                     RLAPPGSAGKVTGATGFVTFAGVVVGPPSFALLSSATGSYRIGFAAFAAASVCAAVAL
                     LARRIQ"
     gene            complement(2969..>3212)
                     /locus_tag="VI319_08950"
     CDS             complement(2969..>3212)
                     /locus_tag="VI319_08950"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY6864015.1"
                     /translation="TSAELLPDVLLVPMLAFDRSGFRLGYGGGFYDRLLPIVPQTAAR
                     VAGAFEMQVVERVPTAPHDVAVDMIVTERRTLNCSA"
BASE COUNT          509 a         1106 c         1094 g          503 t
ORIGIN      
        1 gcgcccgcct cgttgatgcc ctcctggagg atctggccgt gcttgtcctc cttgtagaac
       61 atgagctggt ccttgtcctc gggcgtgtag agctgcccga gctggttcca gatcgcaagc
      121 tggcggaaga gcccttccat cccgaacgtc cgcgactcgt cagggacgat cgggacgatg
      181 tgcttcgcga gattcttgtc gcgcagaagc atctgcagga tctgcacgaa cgccatcgtc
      241 gacgagatct cgcgctcgtc ggtgctcttc aggaaccgct cgaacgcgct gagcggagga
      301 atgaccagcg gctccgcctt ccgccttcgc gcgggcaggt agcctccgag ctccatccgt
      361 cgtgcgcgca tgtactcgag ctcgggagag ccttccgcga acttgacgta cggcacttcc
      421 tcgagcttct cgtcggatac cggaatcgcg aagcgatcgc ggaagcgccg gatcgcctcg
      481 acgttcatct tcttctgctg gtgcgtgacg ttctgcgcct cgccgaagcc gatccccatg
      541 ccgtagccct tgatcgtctt cgcgaggatc accgtcggct gcccgacgtg gttgacggcc
      601 gcgtgatagg cgccgtagac cttgtgcgga tcgtggccgc cgcggttcag gtcccagatg
      661 tccttgtccg aatagtcggc gaccagctcc ttcagctcgg ggctgttgaa gaagtactcg
      721 cggacgtagg cgccgtcctt cgctttgaac gtctggtact cgccgtcgac ggcttccatc
      781 atccgccgca tcaggatgcc cttgcggtcg cgcgtgagca gcgcgtccca gcgcgagccc
      841 cagacgagct tgatcacgtt ccatcccgcg ccgcggaatt cgctctcgag ctcctggatg
      901 atcttgccat tgccgcgcac cggcccgtcg agccgctgca ggttgcagtt gatgacgaag
      961 atcagattgt cgaggttctc ccgcgacgcc atgccgatcg cgcccatcga ctcgggctcg
     1021 tccatctcgc cgtcgccgca gaaacaccag accttgcgcc cctcggtgtt cgcgagcccg
     1081 cggtcgtgca tgtacttcat gaagcgcgcc tggtagatcg ccatcagcgg cccgaggccc
     1141 atcgacaccg tggggaattg ccagaagtcg ggcatcagcc acggatgcgg atacgacgcg
     1201 atgcccttgc cgtcgacctc ctgacggaag ttatcgagct ggctctcggt gagccgcccg
     1261 agcaggaacg cccgcgagta gacgccgggc gacgagtgtc cctggaagaa cacgaggtcg
     1321 ccggcgtgct tgtcggacat tccatgccag aagtggttga agccgacgtc gtagagcgtc
     1381 gcggccgacg catagctcgc gatgtggccg ccgacgttcg tgtgcttgtt tgcgcgcacc
     1441 accatcgcca tcgcgttcca gcgcgtgtac gagcggatcc ggtgctcgat gttctggtcg
     1501 cccgggatgc gcgcctggcg ctcgggcgaa atcgtgttga tgtacgccgt gttcgcggag
     1561 aaggggagat aggcgccgga gcggcgcgcc ttgtcgatca gctgctcgat caggaaatgc
     1621 gcgcggtcgg gcccctgcgt cgccagcacg ccgtcgagcg cctcgagcca ctcgcgggtt
     1681 tcctgcggat cgatgtcggg gatgcggtcc atggtcgact ccgtttgctc gttccgacgg
     1741 tcattgtatg cgcctcgcca gcagggcaac ggccgcgcag acgctggcgg cggcgaacgc
     1801 ggcgaagccg atccggtagc tgccggtcgc gctcgacagc agcgcgaacg acggcggccc
     1861 cacgacgacg cccgcgaacg tcacgaaccc ggtcgcgccc gtcaccttgc ccgcgcttcc
     1921 cggcggcgca agacgtgcga cctcggaaag ctggacgccg ttccagccga tagccgtcgc
     1981 gccgaacagt gcggcgagca cgccgatcgc gagcggcgac cacgccgtcg tcgcgctcgc
     2041 catcagcacg ccgcacgcgc cggcgcagaa tccgatcgtt gccagcacgc gccgcggcgc
     2101 gagccagcgg tcggcgacgt agccccacgc aacgcgcccc gcgacgcccc cgatcgtcgc
     2161 gatcgtgagc gcgaggccgg ccgcgaccag cgagtagccg agcgtctcgg tcaggtagac
     2221 gacgaggaag ctcgtcaggc acacttgcgt cgccgagtag gcgaacgcga cgaacgcgag
     2281 atcgcggagc gcccgcgaat ggcgcagcag gcgaagcggc gcgaagacgc cggcgacgct
     2341 gacgcggccc ttcggattgc ggtcggcgtc gaggccggcc cggatcggct gcgcgaccgc
     2401 gacgatcgcg agtccggcga gtgccgcgat cgcgaacgcg ccgcgccatc cggtcgcgag
     2461 cgcgagtgcc ggcagcagcg cgcccgccag cgccgcaccc gcgggcacgc ccgtctgctt
     2521 gatcgagaac gtgagcgcca ttcgcgcggg cggcgcggtg cgctgcagaa gctgtgacga
     2581 cgcgggcgtg atcggcccgt agcccaggcc gatgacgatg ccgaacgccg cgaccagcgc
     2641 gatcgcgtgc gccggcgcga atgcgatgcc ggcgacgccg agcgcgcaca gcacgacgca
     2701 cgcttgcgag acgcggatcg agccgaggcg ctcgatgaag ccgccggatg cgaggctcgc
     2761 gatcatgccg cccgcgtaga ccaggccgat gaatgcgccg atccagcgcg cctcgacgtc
     2821 gaaggcgagc gcgatctccg gcgcgagcac cgcggccgca gccgccgcga acgcggtgta
     2881 gatctggatg gccagcgttg ccgcgagcgc gatcgccgcg gtgcgcgccg acgtcaaggt
     2941 gctcgcctcg cgcttggggc tctcgccctc atgcgctgca attcagcgtt cgccgctcgg
     3001 tgacgatcat gtcgacggcg acgtcgtggg gcgccgtcgg cacgcgctcg acgacctgca
     3061 tctcgaaggc tcccgcgacg cgcgcggcgg tctgcggcac gatgggcagc agccggtcgt
     3121 agaaaccgcc gccatagccg agccggaagc ctgaacggtc gaaggccagc atcggcacga
     3181 ggagcacgtc cggcagcagc tcggcagaag tc
//
LOCUS       DATDMW010000209        14642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_190493, whole genome shotgun sequence.
ACCESSION   DATDMW010000209 DATDMW010000000
VERSION     DATDMW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14642
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190493"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..997)
                     /locus_tag="VI319_08955"
     CDS             complement(143..997)
                     /locus_tag="VI319_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY6864016.1"
                     /translation="MSSLLRLAAHCPLRLLHALGALAGWAVYLGSPRYRRYIGENLRR
                     AGFGAPAVCRAAVAESGKAVLELPAVWLRPLDAVARLVVDEHGWHHIEAARRSGKGLI
                     LLTPHLGCWEIAGQYIASRIPLTVMYSPPKLRALEPYMVAGRRRQNLKSVPADLSGVR
                     AMLKALKRGEAIGMLPDQVPGVGEGEWVDFFGRPAYTMVLVSRLAEQTGAPVLTVFAE
                     RLPGGRGYRFVVEPLLEARPPESPVRALNRSLEQLIRRRPEQYLWSYNRYKVPAGAAG
                     PGDEAGAP"
     gene            1095..2279
                     /gene="metK"
                     /locus_tag="VI319_08960"
     CDS             1095..2279
                     /gene="metK"
                     /locus_tag="VI319_08960"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY6864017.1"
                     /translation="MSSFLFTSESVSEGHPDKVADQISDAVLDAILAQDKRSRVAAET
                     LVKDNLVVLAGEITTGARVDFGAVARRTIKRIGYTDPSIGFDASTCTVMVAYGEQSQD
                     IAQGVDEGKGLDLEQGAGDQGLMFGYACDETPQLMPMPIYLAHRLVERQAELRRDGRL
                     GWLRPDAKSQVTLRYVDDKPESIETVVLSTQHKPGMAHKQIEEAVVEQVIKPVLPKNL
                     VKGPIKFLVNPTGSFEIGGPKGDCGVTGRKIIVDTYGGSAPHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNVVASGIASRCLVQVSYAIGVAKPTSVMVTTYGTGKISDDKIAELVQK
                     HFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWENTDKAQALAADAGVRKAAM
                     AL"
     regulatory      2372..2455
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            2475..3914
                     /gene="ahcY"
                     /locus_tag="VI319_08965"
     CDS             2475..3914
                     /gene="ahcY"
                     /locus_tag="VI319_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY6864018.1"
                     /translation="MSAVLQSGKSTDYRVADLSLADWGRKEIAIAETEMPGLMAIREE
                     YAATQPLRGARIAGSLHMTIQTAVLIETLKALGADVRWASCNIYSTQDHAAAAIAKSG
                     TPVFAYKGESLEEYWEFTHRIFEWPGKSEQAYANMILDDGGDATLLLHLGTQAESDAS
                     VIAHPSSEEETALFAAIKTRLAKDPKWYSTRIAHIRGVTEETTTGVKRLYRMAKEGAL
                     EFPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVAVVAGYGDVGKGSAQA
                     LRALSAQVWVTEIDPICALQASMEGYRVVTMDYACDKADIFVTATGNFHVITHEHMKR
                     MKNQAIVCNIGHFDNEIDVAGLKQHKWENIKPQVDHVIFPDGKRIILLAEGRLVNLGC
                     ATGHPSYVMSSSFANQTIAQIELWTESQKKSSKYPVGVYVLPKHLDEKVARLQLKKLG
                     AMLTELSDAQARYIGVNREGPYKADHYRY"
     gene            3919..4869
                     /locus_tag="VI319_08970"
     CDS             3919..4869
                     /locus_tag="VI319_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864019.1"
                     /translation="MSLVSKLLARDGFVLLYGTTPPRSGSPDELIRGAAEKLAERVRP
                     LPLDGFVVYDLQDESARTPLPRPFPFAPTVDSRGYAKLLTELTGRSAVCYKCIGRMTH
                     ADWRQWLGDARRDYGVELLTLVGRPSSQKIPYAMTLTQAYSIAAGHDARFVLGGVAIA
                     ERHGVGRDESRRLHDKADVGCRFFVSQTVYQPAATIRMLAGYAALCRKLGRAPCRIVL
                     TFAPCGRPKTMDFMKWLGIAIPEETEREILAAPTPLAKSIEICRENLRQILEQDYVHE
                     LPLGVNTESVSINKEEIDASVDLFHALKDVLDRREQALRT"
     gene            4971..5678
                     /locus_tag="VI319_08975"
     CDS             4971..5678
                     /locus_tag="VI319_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY6864020.1"
                     /translation="MNGLMESEYVFITVNLVAALAAGGIIGLERSHHGRPAGFRTHAL
                     VCLASGLLMLMTVNYTKFAGLAALEGTAPIDPTRMAQGIMTGIGFLGAGVIIKEGMAV
                     RGLTTAASIWVTAGIGVLVGIGFYFAAGIATVLTLGVLSVFRWIERKMPIEFYAKLCV
                     GFLRDARLPEEELRSIVQRQGFIVANMNYSLSGDGRVFQYDMVIHSPVRENTRRLADA
                     FAMMGSVVEFRIAPTGD"
     gene            complement(5696..6637)
                     /locus_tag="VI319_08980"
     CDS             complement(5696..6637)
                     /locus_tag="VI319_08980"
                     /inference="COORDINATES: protein motif:HMM:NF009327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEY6864021.1"
                     /translation="MNFQQLRYVRETVRQGLNLTEAANRLFTSQPGVSKQIRELEKEL
                     GVEIFVRRGKRLVALTEPGRMVVRIIEDLLQQADNLKGAAREFQDQASGTLAVATTHT
                     QARYSLPTVVRQFKRRYPKVHLTLKQGNPPQLAEMVLAGEADVAIATEALDHYPKLLA
                     LPGFQWNHCVVVTAKHPLAKASRVTLEDIARYPVITYDPAFAGRTHIDEAFAARGLHP
                     DIVLSAIDADVIKTYVELDLGIGIVAAMAFDPKRDRGLRALDASHLFKSNTTRIAIRR
                     GAYLRGYAYAFIELFSPQLSREVIDAALAGKGEMYEL"
     gene            6737..7510
                     /locus_tag="VI319_08985"
     CDS             6737..7510
                     /locus_tag="VI319_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY6864022.1"
                     /translation="MDFLYVISGFAVGVIVGITGAGGGSLMTPLLVLLFGVSPVTAVG
                     TDLLFAAITKTGGAWTHARRGGVDWTIVARLAAGSVPAAVLTLVWLHFFVSDQKALSH
                     VVSIGLGVALLLTACALLFREPLRRWAARRGTSILDERATVKRTVVFGAVLGMLVSVS
                     SVGAGALGVTALFFLYPGLAAARIVGTDIAHAVPLTLVAGLGHAAAGVVDWPLLGTLL
                     VGSLPGIWLGSRLGQAMPERFLRGALATMLVLIGGKLVF"
     gene            7514..9229
                     /locus_tag="VI319_08990"
     CDS             7514..9229
                     /locus_tag="VI319_08990"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY6864023.1"
                     /translation="MYIYDEIDQRIVDERVRQFRGQVRRFLDGKLSEDDFRALRLRNG
                     LYMQRHAHMLRISIPYGLLSTRQLRKLAHITRTYDRGYGHFSTRQNLQLNWPKLENVP
                     DILAELASVQMHCIQACGNDLRNITTDHFAGVAPDEIVDPFVWCEIVRQWGALHPEFN
                     FLPRKFKIAVNGAKADRAAVAFHDVGLHAVRADDGAVGFRVLVGGGLGRTPMIGSVIR
                     EFLPWQHLLSYLEAIMRVYNRYGRRDNIHKARIKIVVKERGIEWLRNEVEAEWTHLKD
                     GPSTLLREEVERIESRFTRTSYDKLPGRDPALEAALAGERAFASWHARNVHAHKVPGY
                     AAVTLSLKKTGVPTGDVTSDQMDAIADLADRYSAGELRVSHEQNLIFADVRLRDLHAL
                     WQEVRALGFATPNVGLLTNIICCPGGDLCSLANAKSIPVAEAIQRRFDDLDYLHDIGD
                     IDLNISGCMNACGHHHIGHIGVLGVDKDGEEWYQIEVGGNQGEVRPGVGASLGKVIGP
                     SFARAEVPDVVEQLIACYLERRDSEAERFIDVVHRIGVAPFKEKVYGSTDKTEARRRR
                     QLASA"
     gene            9174..9692
                     /locus_tag="VI319_08995"
     CDS             9174..9692
                     /locus_tag="VI319_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY6864024.1"
                     /translation="MAALIKQKRVVADSWRRLERAADGSLPAVPRQGDVIVPLALWLE
                     RRDDLLAFPGRLGVWLDSHEGPEALVADVGRFEVIAVNFPKYQDGRGYSIARLLRERY
                     GYRGELRAIGEVARDHLFYMAGCGFDAFALREGEDVDEALTAFADFSESYQSSVAQPV
                     PLFRRRLAEGKA"
     gene            9689..10435
                     /locus_tag="VI319_09000"
     CDS             9689..10435
                     /locus_tag="VI319_09000"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY6864025.1"
                     /translation="MSATLQARTDELERVLARAALEHAPSAFASSLGAEDMVITDLIL
                     RRGFDIEIFTLDTGRLHAETLALLEEIRAHYECDVRGRDIRVMRPDAGAVAHYVTVHG
                     LNAFYGSIDLRKRCCEIRKVEPLKRALAGKASWITGQRREQSTTRAALALQEHDAAHG
                     LVKFNPLVDWTEREVWEYIREHGVPYNALYDQGYRSIGCAPCTRPALPGEDVRAGRWW
                     WESPEVRECGLHVAPDGRLVRARKPAETQA"
     gene            10432..11373
                     /gene="cysD"
                     /locus_tag="VI319_09005"
     CDS             10432..11373
                     /gene="cysD"
                     /locus_tag="VI319_09005"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY6864026.1"
                     /translation="MSAVLEQARNEPTPVRARGHLDWLESEAIFILREVAAECERPAL
                     FFSGGKDSIVLLRLAEKAFRPERFPFPLLHIDTGHNFPEVIDFRDRRAAELGERLIVR
                     SVEDSIRRGTVRLRSATDSRNAAQSVTLLEAIAEHGFDACIGGARRDEEKARAKERIF
                     SFRDEWGQWDPKNQRPELWNLFNTRVHKGEHMRVFPISNWTELDVWQYIACERLEVPS
                     IYYAHRRPVVRRRGLLVPVTPLTPAAEGERVEDVQVRFRTVGDVSCTCPVESDAADVA
                     AIIAETARTTVTERGATRMDDQTSDASMERRKKEGYF"
     gene            11375..12658
                     /locus_tag="VI319_09010"
     CDS             11375..12658
                     /locus_tag="VI319_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6864027.1"
                     /translation="MSLLGEIERADAFEPSADDGVLRFITAGSVDDGKSTLIGRLLHD
                     SRAVLDDQLAAVERASRKRGADALDLSLLTDGLEAEREQGITIDVAYRYFATPRRKFI
                     IADTPGHEQYTRNMVTAASTADAAVILIDAGKGVLPQTKRHLTLANLLGVRHILVAVN
                     KMDAVGWDRGVFERVCEDLRRFAAPLSLGELRFFPVSALRGDFVVQRGEHAPWYAGPP
                     LLEALEGLDLDDDAVALPLRFPIQLVSRPGAAGIRRYAGTLASGTLAPGTEVEVLPSG
                     RRTAVRAVATFEGRRTLATAGDAVTLTLSDELDVSRGDMLVDVRRPPRASKSVRAEIC
                     WLAQSPLDPAGRYLLKHTTRTVRARVVTVNHRLDIHTLERAAPDAIGTNDIAGVSLSL
                     AQPIFCDPYRENRATGSFILIDEITHQTVAAGLVE"
     gene            12655..13419
                     /gene="cobA"
                     /locus_tag="VI319_09015"
     CDS             12655..13419
                     /gene="cobA"
                     /locus_tag="VI319_09015"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY6864028.1"
                     /translation="MTGTVYLIGAGPGAPDLLTLRAAGILGRADVVFYDALIHPGTLA
                     LAVRAEKIAVGKRSGRHSTAQRFINKQLVDAARKHTVVVRLKGGDPLLFGRAQEEIDA
                     LRSAEVPFEIVPGVTAASAAGAELGVSLTRRGVARKVAFVTPRAGAGEPANEWASSVA
                     AADTAVIYMGAGLAETLVRELAHRGMRADTPLAIVENASLPGAKILTGTLEQLPGLAG
                     SLGSGPAVVVLGEVVRAARAAGASLPARLRPLLKRA"
     gene            13495..13719
                     /locus_tag="VI319_09020"
     CDS             13495..13719
                     /locus_tag="VI319_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864029.1"
                     /translation="MASTKNPEPVWRSPTGDVVSCVEKLKVLRENLDEIRQMCQDALE
                     DAVLMGCDEGQFKDVLAHVVQALENPYKDR"
     gene            13722..14165
                     /locus_tag="VI319_09025"
     CDS             13722..14165
                     /locus_tag="VI319_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864030.1"
                     /translation="MSRAAALALLFALPFLALAQARETERALLERQQQSDRFSLQLRQ
                     SQSVLGAPAAERLDLDARYLGQRQAQDRLHDAQLREVVQQPEAMRSGGPDRYERDRRS
                     QNLGVVTPPQHGRPDPDAGVRWTPTLEDRSPDGVSWTPTLEKRRR"
     gene            complement(14176..>14642)
                     /locus_tag="VI319_09030"
     CDS             complement(14176..>14642)
                     /locus_tag="VI319_09030"
                     /inference="COORDINATES: protein motif:HMM:NF003354.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY6864031.1"
                     /translation="GNAIGFLVCAGLLAFAYYLQFARGLEPCPLCILQRVVFAAMAIF
                     FLVAAIHRPRRVGAWIYGVLLFALAVTGTAIASRHVWLQHTPEAERPSCGPGLDYLLS
                     RFGPLGGLQRILRGSGECGAVDWTFLGLSIPEWSLACFIALGIYALVLAARD"
BASE COUNT         2352 a         5095 c         5016 g         2179 t
ORIGIN      
        1 cagcgctcga ggttgaccag gcacacgcgc cggcgctcgc gcgcgagcgg gaagagcagc
       61 ctgccgacgc cctcgccgat gcgcgcgagc aggccgagcg gcagcaggtg cagcagccac
      121 atgaacgcca atgcgagccg catcatggcg cgcccgcctc gtctccgggc ccggcagcgc
      181 ccgcgggcac cttgtagcgg ttgtacgacc acagatactg ctcgggccgc cggcggatga
      241 gctgctcgag cgagcggttg agcgcgcgta ccggcgactc cggcggccgc gcctccagca
      301 acggctcgac cacgaagcga taaccgcgtc cgcccggcag ccgctcggcg aagacggtca
      361 gcacgggcgc gcccgtctgc tcggcgagac gcgacacgag caccatggtg taggccgggc
      421 ggccgaagaa gtccacccac tcgccctcgc cgacgccggg cacctggtcc ggcagcatgc
      481 cgatcgcctc gccgcgcttg agcgctttca gcatcgcgcg cacgccgctc agatcggcgg
      541 gcacgctctt caggttctgg cggcggcggc cggcgaccat atagggctcg agggccctga
      601 gcttgggcgg gctgtacatg accgtgagcg ggatccggct cgcgatgtac tggcccgcga
      661 tctcccagca gcccaggtgc ggcgtgagca ggatgaggcc cttgcccgag cgacgggcgg
      721 cttcgatgtg atgccagccg tgctcgtcga ccaccaggcg cgcgaccgcg tcgagcggac
      781 gcagccacac cgccggaagc tcgagcaccg ccttgccgct ctcggcgacc gcggcccggc
      841 acacggcggg cgcgccgaag ccggcgcggc gaagattctc cccgatatat cgccggtagc
      901 gcggcgagcc gaggtagacc gcccagccgg cgagcgcgcc caatgcgtgc agcagccgca
      961 gtgggcagtg ggccgcgagc cgcagcaacg acgacatttg tggttctgat ggagtgggat
     1021 gtaggccgat tgggtgggcg aaattcgcgt gctacactgc gccctcttct tcttacctac
     1081 gacgccgccc gaacatgagc agctttctct tcacgtccga atccgtctcc gagggtcatc
     1141 cggacaaggt cgccgaccag atttcggacg ccgtcctcga cgccattcta gcccaggaca
     1201 agcgctcgcg cgtcgccgcc gagacgctgg tgaaggacaa cctcgtcgtg ctcgcgggag
     1261 aaatcacgac cggcgcgcgc gtcgatttcg gcgccgtggc gcgcaggacg atcaagcgca
     1321 tcggatacac cgatccgtcg atcggcttcg atgcctcgac ctgcacagtg atggtcgcct
     1381 acggcgagca gtcgcaggac atcgcgcagg gcgtcgacga aggcaagggc ctggatctcg
     1441 agcagggcgc gggcgaccag ggcctcatgt tcggctacgc ctgcgacgag actccgcagc
     1501 tgatgccgat gccgatctac ctcgcgcacc gcctggtcga gcgccaggcc gagctgcgcc
     1561 gcgacgggcg cctcggctgg ttgcgtcccg acgcgaagtc tcaggtcacg ctgcgctacg
     1621 tcgacgacaa gcccgaatcg atcgagacgg tggtgctctc cacgcaacac aagcccggaa
     1681 tggcgcacaa gcagatcgag gaagcggtgg tcgagcaggt gatcaagccc gtgttgccga
     1741 agaacctcgt gaagggcccg atcaaattcc tcgtcaatcc gaccggcagc ttcgagatcg
     1801 gcgggccgaa gggcgactgc ggcgtgaccg ggcgcaagat catcgtcgac acctacggcg
     1861 gctcggcgcc gcacggcggc ggcgcctttt cgggcaagga tccctcgaag gtcgatcgct
     1921 cggccgcgta cgcggcgcgc tacgtcgcca agaacgtggt tgcctcgggc atcgcctcgc
     1981 gctgcctggt gcaggtgtcg tacgcgatcg gggtcgcgaa gccgacctcg gtcatggtga
     2041 ccacgtacgg gaccggcaag atctccgacg acaagatcgc cgagctggtg cagaagcact
     2101 tcgacctgcg gccgaaaggc atcatccaga tgctcgacct cctgcgcccg atctacgaga
     2161 agaccgcggc gtacggtcac ttcgggcgcg aggagcccga gttcacctgg gagaacaccg
     2221 acaaggcgca ggcgctcgcc gccgacgcgg gcgtgcgcaa ggccgcgatg gcgctgtagc
     2281 ggttccgcga caccgaggaa gcccgggcgc gacccgggct ttgttttttc cgcggctggc
     2341 gacgcccgtc gccggtgcgg tagaatctgc cttcctcgag gagcgttgca acgagccggg
     2401 acccgcgccg gccgccaggc tcgaggattc aacaccgcaa cggcgctcac gaactctgta
     2461 gaaaggaggg cgtgatgagc gccgtactgc aatccggaaa atccaccgac taccgcgtcg
     2521 ccgatctgtc gctcgccgac tgggggcgga aggagatcgc catcgccgag accgagatgc
     2581 ccggtctgat ggcgatccgg gaagaatatg cggccacgca gccgctgcgc ggcgcgcgta
     2641 tcgcgggctc gctgcacatg acgattcaaa ctgccgttct cattgagacg ctgaaggcgc
     2701 tcggggccga cgtgcgctgg gcttcctgca atatttattc gacgcaggat cacgccgcgg
     2761 cggcgatcgc gaagtcggga acgccggtat tcgcctacaa gggcgaatcg ctcgaggagt
     2821 actgggagtt cacgcaccgc attttcgaat ggcccgggaa gagcgagcag gcctacgcca
     2881 acatgatcct cgacgacggc ggcgacgcga ccctgctgct gcacctcgga acccaggcgg
     2941 agtccgacgc ctcggtgatc gcgcacccct cgagcgagga ggaaacggcg ctcttcgccg
     3001 cgatcaagac gcgcctcgcc aaggacccga agtggtactc gacgcgcatc gcgcatatcc
     3061 gcggcgtgac cgaggagacc acgaccggcg tcaagcgact gtaccgcatg gcgaaggaag
     3121 gcgcgctcga gttcccggcg atcaacgtca acgactcggt gacgaagtcg aagttcgaca
     3181 acctctacgg ctgccgcgag tcgctggtcg acggcatcaa gcgcgcgacc gacgtgatga
     3241 tcgcgggcaa ggtcgccgtc gtcgccggct acggcgacgt cggcaagggc tcggcgcagg
     3301 cgctgcgcgc gctgtccgcg caggtctggg tgaccgagat cgacccgatc tgcgcgctgc
     3361 aggcgtcgat ggaaggctac cgcgtcgtga ccatggacta cgcctgcgat aaggccgaca
     3421 tcttcgttac cgccacgggc aacttccacg tcatcacgca cgagcacatg aagcgcatga
     3481 agaaccaggc gatcgtgtgc aacatcggcc acttcgacaa cgagatcgac gtcgccggcc
     3541 tgaagcagca caaatgggag aacatcaagc cgcaggtcga ccacgtcatc tttcccgacg
     3601 gcaagcggat catcctgctc gccgaagggc gcctggtgaa cctcggctgc gccaccggcc
     3661 acccgagcta cgtgatgagc tcgtcgttcg ccaaccagac gatcgcgcag atcgagctct
     3721 ggaccgagtc gcagaagaag tcgagcaagt acccggtcgg cgtctacgtg cttcccaagc
     3781 acctcgacga gaaggtcgcg cgcctgcagc tgaagaagct cggcgcgatg ctgaccgagc
     3841 tgagcgatgc gcaggcgcgc tacatcgggg tgaaccggga aggcccgtac aaggcggacc
     3901 actaccgcta ctgaggctgt gagcctcgtt tcgaagctgc tcgcgcgcga cggcttcgtg
     3961 ctgttgtacg gcacgacccc gccgcgctcg ggctcgccgg acgagctgat ccgcggcgcc
     4021 gcggaaaagc tggccgagcg cgtgcggccg ctgccgctcg acgggttcgt cgtctacgat
     4081 ctccaggacg agagcgcgcg cacccccttg ccgcgcccgt tcccgttcgc gccgaccgtg
     4141 gattcgcgcg gctacgcgaa gctgctcacc gagctcaccg ggcgctccgc cgtgtgctac
     4201 aagtgcatcg gccgcatgac gcacgcggac tggcgtcagt ggctcggcga cgcgcgccgc
     4261 gattacggcg tcgagctgct cacgctcgtg ggccggccga gctcgcagaa gatcccgtat
     4321 gccatgacgc tcacgcaggc ctactcgatc gcggcgggtc acgacgcgcg cttcgtcctg
     4381 ggcggcgtgg cgatcgccga gcggcacggc gtcgggcgcg acgagagccg gcggctgcac
     4441 gacaaggccg atgtcggctg ccgcttcttc gtctcgcaga cggtctacca gcccgccgcc
     4501 accatccgca tgctcgccgg ctacgccgcg ctgtgccgca agctcgggcg cgcgccctgc
     4561 cgtatcgtgc tgaccttcgc tccctgcggg cgcccgaaga ccatggactt catgaagtgg
     4621 ctcggcatcg ccatccccga ggagaccgag cgcgagattc tcgcggcgcc gacgccgctc
     4681 gcgaagtcga tcgagatctg ccgcgagaac ctgcggcaga tcctcgagca ggactacgtc
     4741 cacgagctgc ccctgggcgt gaacaccgag agcgtctcga tcaacaagga ggagatcgac
     4801 gcgtcggtcg acctgttcca cgcgctcaag gatgtcctcg accgccgcga gcaggcattg
     4861 cgcacgtaga cgccgacacg tcggttggag gacgagcgcg ggcgcgctat gctatcggcg
     4921 gcaccgcgcc atgtcgtctc ggcagcgcgg cacccgggga gagaagaaca atgaacggcc
     4981 tgatggaaag cgaatacgtc ttcatcacgg tcaacctggt cgctgccctc gcggcgggcg
     5041 ggatcatcgg actcgagcgc agccaccatg gccgtcccgc cggattccgg acgcacgcgc
     5101 tcgtctgcct cgcctccggg ctgctgatgc tcatgaccgt gaactacacc aaattcgccg
     5161 gcctcgcagc tctcgaaggc accgctccga tcgacccgac gcgcatggct caggggatca
     5221 tgaccggcat cggcttcctg ggcgccggcg tgatcatcaa ggaaggcatg gcggtccgcg
     5281 gactgaccac cgcggcgtcg atctgggtca cggcaggcat cggcgtgctg gtcggcatcg
     5341 gcttctactt cgccgccggc atcgcgaccg tgctgacgct gggcgtgctg tcggtgttcc
     5401 gctggatcga gcgcaagatg ccgatcgagt tctacgccaa gctgtgcgtg ggcttcctgc
     5461 gcgatgcgcg gcttcccgag gaggagctgc gctcgatcgt ccagcgccag ggcttcatcg
     5521 tcgccaatat gaactacagc ctgtccgggg acggcagggt gttccagtac gacatggtca
     5581 tccactcgcc ggtgcgcgag aacacgcggc gcctcgccga cgccttcgcg atgatgggct
     5641 cggtggtcga gttccgcatc gcgcccacgg gagactgagc ggcgcccggc gccccctaca
     5701 gctcgtacat ctcgcccttg ccggcgagcg cggcgtcgat cacctcgcgt gagagctggg
     5761 gtgagaacag ctcgatgaac gcataggcgt agccgcgcag gtaggcgccg cgccggatcg
     5821 cgatgcgcgt cgtattgctc ttgaacaggt gcgacgcgtc gagcgcgcgc agcccgcggt
     5881 cgcgcttcgg gtcgaacgcc atggcggcga cgatgccaat gcccaggtcg agctcgacgt
     5941 aggtcttgat cacgtccgcg tcgatcgcgg acagcacgat atcgggatgc agcccgcgcg
     6001 ctgcgaacgc ctcgtcgatg tgcgttcggc cggcgaacgc gggatcgtag gtgatcaccg
     6061 gatagcgcgc gatgtcctcg agcgtcacgc gcgaggcctt ggcgagcgga tgcttcgccg
     6121 tcacgaccac gcagtggttc cactggaagc cgggtagcgc gagcagcttc gggtaatggt
     6181 ccagggcttc cgtggcgatt gcgacgtcgg cctcgcccgc gagcaccatc tcggcgagct
     6241 gcggcggatt cccctgcttc agcgtgaggt gcaccttcgg atagcgtcgc ttgaactggc
     6301 gcacgaccgt cggcagcgag tagcgcgcct gggtgtgagt ggtcgcgacc gccagcgtcc
     6361 cgctcgcctg gtcctggaac tcgcgcgccg cgcccttcag gttgtcggcc tgctgcagca
     6421 agtcctcgat gatcctcacc accatgcgtc ccggctcggt cagcgccacc aggcgcttgc
     6481 cccggcgcac gaagatctcg acgccgagct ccttctcgag ctcccggatc tgcttcgaca
     6541 cgcccggctg cgaggtgaac agtctgttcg cggcctcggt gagattcagt ccctggcgca
     6601 cggtctcccg gacgtagcgc aactgctgga aattcatacg gttatgttta tgccatgaag
     6661 ttattaaatt ctaacatgaa agtcttttga gctattaggg ccgggcgcta cgatcgtccc
     6721 tcgctcggag aaacgcgtgg acttcctgta tgtaatttcc ggtttcgcgg tcggcgtcat
     6781 cgtcgggatc accggcgcgg gcggcggctc gctgatgacg ccgctgctcg tgctgctgtt
     6841 cggcgtctcg ccggtgaccg cggtcggcac cgacctgctc ttcgccgcga tcaccaagac
     6901 gggcggcgcc tggacccacg cccgccgcgg cggcgtcgac tggaccatcg tcgcgcggct
     6961 cgccgcgggc agcgttccgg ccgccgtgct gaccctcgtc tggctgcatt tcttcgtctc
     7021 cgatcagaag gcgctctctc acgtggtgag catcggactg ggcgtggctc tgcttctcac
     7081 cgcatgcgcg ctgttgttcc gcgagccgct gcgccgctgg gcggcgcgcc gtggcacctc
     7141 gatcctcgac gagcgggcga ccgtgaagcg cacggtcgtg ttcggtgccg tgctcggcat
     7201 gctcgtgagc gtgtcttcgg tcggcgccgg tgccctcggg gtgacggcac tgttcttcct
     7261 ctatcccggg ctcgccgcgg cgcgcatcgt gggaaccgac atcgcgcacg cggtaccgct
     7321 cacgctggtc gcgggcctcg ggcatgcggc cgcgggcgtc gtcgactggc cgctgctcgg
     7381 caccctgctc gtgggctcgc ttcccggcat ctggctcggg agtcggctcg gccaggcgat
     7441 gcccgagcga tttctgcgcg gcgcgcttgc gacgatgctg gtcctgatcg gcggaaaact
     7501 ggtgttctga cccatgtata tctacgacga aatcgaccag cgcatcgtcg acgagcgcgt
     7561 gcgccagttc cgcgggcaag tgcggcgctt cctcgacggc aagctctcgg aggacgactt
     7621 ccgcgcgctg cggctgcgca acggcctcta catgcagcgc catgcgcaca tgctgcgcat
     7681 ctccattccc tacgggctgc tgtcgacgcg ccagctgcgc aagctcgcgc acatcacccg
     7741 tacgtacgac aggggctacg gacacttcag cacgcgccag aacctgcagc tcaactggcc
     7801 caagctcgag aacgtgcccg acatcctcgc cgagctggcg agcgtgcaga tgcactgcat
     7861 ccaggcctgc ggcaacgacc tgcgcaacat cacgaccgac catttcgcgg gcgtcgcgcc
     7921 cgacgagatc gtcgatccct tcgtgtggtg cgagatcgtg cgtcagtggg gcgcgctgca
     7981 ccccgagttc aacttcctgc cgcgcaagtt caagatcgcc gtgaacggcg cgaaggccga
     8041 ccgcgccgcg gtcgcgttcc acgacgtcgg cctgcacgcc gtgcgcgccg acgacggtgc
     8101 cgtcgggttc cgggtgctcg tcggcggcgg cctcggccgc acgccgatga tcggctcggt
     8161 gatccgcgag ttcctgccgt ggcagcacct gctcagctac ctcgaggcga tcatgcgggt
     8221 ctacaaccgc tacggccgcc gcgacaacat ccacaaggcg cgcatcaaga tcgtcgtgaa
     8281 ggagcgcggc atcgaatggc tgcgcaacga ggtcgaagcc gagtggacgc acctcaagga
     8341 cgggccgtcg acgctgctgc gcgaggaggt cgagcggatc gagtcgcgct tcacgcgcac
     8401 gagctatgac aagcttcccg ggcgcgatcc cgcgctggag gccgccctcg ccggcgagcg
     8461 cgccttcgcc agctggcatg cgcgcaacgt gcacgcgcac aaggtgccgg gctacgccgc
     8521 ggtgacgctc tcgctcaaga agaccggcgt gccgaccggc gacgtcacct ccgaccagat
     8581 ggacgcgatc gccgacctcg ccgaccgcta ctcggccggc gagctgcgcg tctcgcacga
     8641 gcagaacctg atcttcgccg acgtgcgcct gcgcgacctg cacgcgctgt ggcaggaagt
     8701 gcgcgcgctc gggttcgcga cgcccaacgt cggactgctc accaacatca tctgctgccc
     8761 cggcggggac ctctgctccc tcgccaacgc gaagtcgatc ccggtcgccg aggcgatcca
     8821 gcgccgcttc gacgacctcg actatctgca cgacatcggc gacatcgacc tcaacatctc
     8881 gggctgcatg aacgcctgcg gccaccacca catcggccac atcggcgtgc tcggcgtcga
     8941 caaggacggc gaggagtggt accagatcga ggtcggcggc aaccagggcg aggtgcgccc
     9001 cggagtaggc gcctcgctcg gcaaggtcat cggaccgtcg ttcgcgcgcg ccgaggtgcc
     9061 cgacgtggtg gaacagctga tcgcctgcta cctcgagcgc cgcgacagcg aggccgagcg
     9121 cttcatcgac gtcgtgcacc gcatcggcgt cgccccgttc aaggaaaagg tctatggcag
     9181 cactgataaa acagaagcgc gtcgtcgcag acagctggcg tcggcttgag cgcgcggcgg
     9241 acggctcgct gcccgcggtc ccgcggcagg gagacgtcat cgtcccgctc gcgctgtggc
     9301 tcgagcggcg cgacgatctg ctcgccttcc ccgggcggct cggcgtctgg ttggacagcc
     9361 acgagggccc agaggcgctc gtcgccgacg tcggccgctt cgaggtgatc gccgtgaact
     9421 tcccgaagta ccaggacggg cgcggctact cgatcgcgcg gctgctgcgc gagcgctacg
     9481 gctaccgcgg tgagctgcgc gcgatcggcg aggtcgcgcg cgaccatctg ttctacatgg
     9541 ccggctgcgg cttcgacgcc ttcgcgctgc gcgagggcga ggacgtcgac gaggccctca
     9601 cggcgttcgc ggacttcagc gagagctacc agtcctcggt ggcacagccc gtgccgctct
     9661 tccgccgccg gctcgcggag ggcaaggcat gagcgcgacg ctgcaggcgc gcaccgacga
     9721 gctcgagcgc gtgctggcgc gagcggcgct ggagcacgcg ccttcggcgt tcgcctcgag
     9781 cctcggcgcc gaggacatgg tcatcaccga cctcatcctg cgccgcggct tcgacatcga
     9841 gatcttcacg ctcgacacgg gccggctgca cgcggagacg ctcgcgctcc tcgaggagat
     9901 ccgcgcgcac tatgaatgcg acgtgagagg ccgcgacatc cgcgtgatgc gcccggacgc
     9961 cggcgcggtg gcgcactacg tcaccgtcca cggcctcaac gccttctacg gcagcatcga
    10021 tctgcgcaag cgctgctgcg agatccgcaa ggtcgagccg ctcaagcgcg cgctcgccgg
    10081 gaaggcctcg tggatcacgg gacagcgtcg cgagcagagc acgacgcgcg cggcgctcgc
    10141 gctgcaggag cacgacgcgg cccacggcct cgtcaagttc aatcccctcg tcgactggac
    10201 cgagcgcgag gtgtgggaat acatccgcga gcacggcgtc ccgtacaacg cgctgtacga
    10261 tcagggctac cgctcgatcg ggtgcgcgcc gtgcacgcgc cccgcgctgc cgggcgagga
    10321 cgtgcgcgcc ggccgctggt ggtgggaatc tcccgaggtc cgcgagtgcg gtctgcacgt
    10381 tgctcccgac gggcgcctgg tgcgcgcccg caagcccgcg gagacgcagg catgagcgcg
    10441 gtcctcgagc aagcccggaa cgagcccact cccgtccgcg cgcggggcca cctcgactgg
    10501 ctcgagtccg aggcgatctt catcctgcgc gaggtcgcgg ccgagtgcga gcgtccggcg
    10561 ctcttcttct cgggcggcaa ggactcgatc gtgctcctgc gcctcgcgga aaaggcgttc
    10621 cggcccgagc gcttcccctt cccgctgctg cacatcgaca ccggccacaa cttccccgag
    10681 gtgatcgatt tccgcgaccg gcgcgcggcc gagctcggcg agcggctgat cgtgcgctcg
    10741 gtcgaggact cgatccgccg cggcaccgtg cgcctacgct ccgcgaccga cagccgcaac
    10801 gccgcgcagt cggtgacgct gctcgaggcg atcgccgagc acggcttcga cgcctgcatc
    10861 ggcggcgcgc gccgcgacga ggagaaggcg cgcgccaagg agcgcatctt ctcgttccgg
    10921 gacgaatggg gccagtggga cccgaagaac cagcggcccg agctctggaa cctcttcaac
    10981 acgcgcgtgc acaagggcga gcacatgcgt gtgtttccga tcagcaactg gaccgagctc
    11041 gacgtctggc agtacatcgc gtgcgagcgg ctcgaggtgc cgtcgatcta ctacgcgcac
    11101 cgacgtccgg tcgttcgacg ccgggggctg ctggtcccgg tgaccccgct gacgccggcc
    11161 gccgaaggcg agcgcgtcga ggatgtgcag gtgcgcttcc gcaccgtcgg cgatgtcagc
    11221 tgtacctgcc cggtcgaatc cgatgcggcg gacgtggccg cgatcatcgc ggagaccgcg
    11281 cgcaccacgg tgaccgagcg cggcgcgacg cgcatggacg accagacctc ggacgcgtcg
    11341 atggagcgcc gcaagaaaga aggatatttc tagaatgtcg ctgctcggcg aaatcgagcg
    11401 cgcggacgcg ttcgagccat cggcggacga cggggtgctg cggttcatca cggccggcag
    11461 cgtcgacgac ggcaagagca cgctgatcgg gcgcctgctg cacgacagcc gcgcggtgct
    11521 cgacgatcag ctcgccgcgg tcgaacgcgc ttcgcgcaag cgcggcgccg acgccctcga
    11581 cttgtcgctc ctgaccgacg gcctggaggc cgagcgcgag cagggcatca ccatcgacgt
    11641 tgcctaccgc tatttcgcga cgccgcggcg caagttcatc atcgcggaca cgcccggcca
    11701 cgagcagtac acgcgcaaca tggtcacggc ggcgagcacc gccgacgccg cggtgatcct
    11761 gatcgacgcg gggaaagggg tactgcccca gaccaagcgg cacctgacgc tcgccaacct
    11821 gctcggcgtg cgtcacatcc ttgtcgcggt gaacaaaatg gacgccgtcg gctgggaccg
    11881 gggcgtcttc gagcgggtgt gcgaggacct gcgccgcttc gcggctccgc tctcgctcgg
    11941 cgagctcagg ttctttccgg tgtccgcgct gcggggcgat ttcgtggtcc agcgcggcga
    12001 gcacgcgccc tggtatgcgg gtccgccgct gctcgaagcg ctcgaaggtc tcgacctcga
    12061 cgacgatgca gtggcgctgc cgctgcggtt cccgatccag ctggtgtcgc ggccgggagc
    12121 ggccggcatc cggcgctacg ccgggacgct cgcctcgggc acgctggcgc cggggaccga
    12181 ggtcgaggtg cttccctcgg gccgtcgcac cgcggtgcgc gcggtggcga ccttcgaagg
    12241 ccggcggacg ctcgcgacgg cgggcgacgc cgtgacgctg acgctgagcg acgagctcga
    12301 cgtctcgcgc ggcgacatgc tcgtcgacgt gcgccgtccg ccgcgcgcgt cgaaatccgt
    12361 gcgcgccgag atctgctggc tcgcgcagtc gccgctcgat cccgcgggcc gctatctcct
    12421 caagcacacg acgcgcacgg tgagggctcg cgtcgtcacc gtgaaccacc gtctggacat
    12481 ccataccctc gagcgtgccg cgcccgacgc gatcggcacg aacgacatcg ccggcgtatc
    12541 gctgtcgctg gcccagccga tcttctgcga tccctaccgc gagaaccgcg cgaccgggtc
    12601 gttcatcctg atcgacgaga tcacccacca gaccgtcgcc gcgggactgg tcgaatgacg
    12661 ggcaccgtat acctgatcgg cgcgggtccc ggggcgccgg acctgctgac gctgcgcgcc
    12721 gccggcatcc tcggacgcgc cgacgtcgtg ttctacgacg cgctcatcca cccggggacg
    12781 ctggcgctcg cggtacgcgc cgagaagatc gccgtcggca agcgcagcgg ccggcactcc
    12841 acggcgcagc gcttcatcaa caagcagctc gtcgacgcgg cgcgcaagca cacggtcgtc
    12901 gtgcggctga agggcggcga tccgctgctc ttcgggcggg cacaggagga gatcgacgcg
    12961 ctgcgctcgg cggaggtccc gttcgagatc gtcccgggcg tcaccgcggc gtcggcagca
    13021 ggggccgagc tcggcgtctc gctcacgcgc cgcggcgtcg cgcgcaaagt cgcgttcgtg
    13081 acgccgcgcg cgggcgccgg cgagccggcg aacgaatggg cctcgagcgt cgccgccgcc
    13141 gatacggcgg taatctacat gggcgcgggg ctggcggaga ccctggtgcg cgagctcgcg
    13201 caccgcggca tgcgcgccga cacgccgctc gcgatcgtcg agaacgcgtc cttgcctggg
    13261 gcgaagatcc tgacggggac tctcgagcag ctgcccgggc tcgcggggag cctcggctcc
    13321 gggcccgccg tggtcgtcct gggcgaagtc gtgcgcgcgg cgcgggccgc gggcgcctcg
    13381 ctcccggcgc ggctgcgccc gctcttgaag cgcgcctgag ccggccgcac aattcctagt
    13441 atcgaaaccc cacgagcgac acggggtctc atccctatac gacgagacag cgatatggcc
    13501 tccaccaaaa accccgagcc ggtctggcgc tcgcccacgg gcgacgtcgt ctcctgcgtg
    13561 gagaagctca aggtcctgcg cgagaacctc gacgagatcc ggcagatgtg ccaggacgcg
    13621 ctcgaggacg cggttctcat gggctgcgac gaggggcagt tcaaggacgt gctcgcgcac
    13681 gtggtgcagg cgctggagaa tccctacaag gaccgctgac cgtgtcgcgc gccgcggccc
    13741 tggcactgct cttcgcgctc cccttcctcg cgctggcaca ggcgcgcgag accgagcgcg
    13801 cgctgctcga gcggcagcag caatcggatc gcttctcgct gcagctgagg caatcgcaat
    13861 cggtgctggg tgcgcccgcc gcggagcgtc tcgacctcga cgcgcgctac ctcggacagc
    13921 gccaggcgca ggatcggctt cacgacgcgc agctgcgcga ggtggtgcag cagcccgagg
    13981 cgatgcgctc gggcgggccc gaccgctacg agcgcgatcg gcgctcgcag aacctcggtg
    14041 tcgtcacgcc gccgcagcac ggccgccccg atccggacgc gggcgtgcgc tggaccccga
    14101 cgctggaaga ccggtctccg gacggcgtga gctggacgcc gacgctggaa aagcgccggc
    14161 gttagcgccg ccggctcaat cgcgcgccgc gagcacgagc gcgtagatcc cgagcgcgat
    14221 gaagcatgcg agcgaccact cggggatgct gagacccagg aaggtccagt cgaccgcgcc
    14281 gcattcgccc gagccgcgca ggatgcgctg caggccgccc agcggcccga agcgcgagag
    14341 cagatagtcc agtcccggac cacacgaggg gcgctcggcc tcgggcgtgt gctgcagcca
    14401 gacgtgccgg ctggcgatgg ccgtgcccgt caccgcgagc gcgaagagca gcacgccgta
    14461 gatccacgcg ccgacccggc gagggcggtg gatcgcggcg accaggaaga aaatggccat
    14521 cgcagcgaac accacgcgct gcaggatgca cagcgggcag ggctcgaggc ccctggcgaa
    14581 ctgcaggtag tacgcgaacg cgagcagccc cgcgcacacc aggaacccga tcgcgttgcc
    14641 gg
//
LOCUS       DATDMW010000210         4524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_65773, whole genome shotgun sequence.
ACCESSION   DATDMW010000210 DATDMW010000000
VERSION     DATDMW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65773"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..573
                     /locus_tag="VI319_09035"
     CDS             247..573
                     /locus_tag="VI319_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864032.1"
                     /translation="MQAKTLIAVLVAGLFAAPIAFAQTGGTGPSGMDQRSADEQTPPK
                     ADQEKSTTGRSTAPKAKTSKRSSKRDHERLSRNDRGTAADTPNMARSPRTSGNPPKQG
                     SSPEPQ"
     gene            693..1673
                     /locus_tag="VI319_09040"
     CDS             693..1673
                     /locus_tag="VI319_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864033.1"
                     /translation="MKMAKAVLAAAALCLAGAACAQSAASGSGYPVKAIKLLVGFPPG
                     SAADVVARIVSPRLGETLGQPLVVENRAGASSSIAAEAVVRSPADGYALFFGSVANAI
                     NASLNKDLSFDFVKDLAPVAGLTTMPNLLVVHPSLEVRSVAELIAAARAKPDAILYGS
                     SGNGTSPHLSGELFNLAAGVKMVHIPYKGSPQAVTDLLAGRVQVMFSPASTVLAHIKA
                     GKLRALASTGAQRTAAAPDLPTVSESGLPGFETSIWFGLFAPAATPRDVIDRIARETA
                     RVLAAPDVRAQLARQGVDPMPKTPEQLGEYVRDEVDKWGQVVRNAGVRAD"
     gene            1693..2655
                     /locus_tag="VI319_09045"
     CDS             1693..2655
                     /locus_tag="VI319_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864034.1"
                     /translation="MNPFVRFVVADALASAPLVALAQDYPAKPIKLLIGFPPGGNVDV
                     VGRIVAAKMSEGLGQQVVPENRTGAGGIIANDAVAKAAPDGYTLLLVSGAHVTQAAVR
                     ASLPYDPVRAFAWVSTVVKYPLVIEVRSDSSFKSLDDFIAYVKGNPGKVYYPSPGMGT
                     LYHLAAEQFIAMAGIEMQHVPFRGGAEPLTEVLAGRMPMLFDALTNSYPQIRAGKFRP
                     LAVTSAEPSAALPGVPTAAHSVPGYEASSFSGIATTAGTPPAVIERLNRELRRVCALP
                     DVAQRFAEWGGQPEASSPEEMRAYVEREIAKWKRVVEQRKIELQ"
     gene            2678..3088
                     /locus_tag="VI319_09050"
     CDS             2678..3088
                     /locus_tag="VI319_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6864035.1"
                     /translation="MKKSRLAIAAAFAAGAVFGALGIQSLNAQQPGFKRALLQKHELS
                     APGRDAVVATAEFEPGVAAGRHTHPGEELSYVLEGTVLLEVTGQPPRTLHAGDVFFIP
                     PNTIHDGKNIGKGPAKVLATYIVERGKPVATPAK"
     gene            3155..4291
                     /locus_tag="VI319_09055"
     CDS             3155..4291
                     /locus_tag="VI319_09055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY6864036.1"
                     /translation="MRLGYFTMPLHPLHRDPAQTLQEDREAIILADRLGFHDAFVGEH
                     LTDRAENVTSSFIFLASLIAETKTIRLATGTSNLSQSHPVVIAAQAAMFDHLARGRFI
                     FGVSPGALSSDAEALGILEQDRNRMFAECIDVILEIWERDPPYDIELAGNRWKVTTAK
                     TYVPDIGRGVMYKPYQKPRPEIVGTVVAPFSKGVIAMGERDFHPLSANFLLPHWLPSH
                     WTNYVEGKRRAGKAADPSDWRVARTIFVADDAKTARRYAVEDPASPYAFYWKQLRTKM
                     MLAKRHVIFKMREDEDDAAVTLERLLDRLVIHGTPEQVAERIVALRAQAGPFGEIVYA
                     GMDWVDAGLARRSMELMATEVMPRVNAALKEGQWKSASISSPAA"
     gene            4255..>4524
                     /locus_tag="VI319_09060"
     CDS             4255..>4524
                     /locus_tag="VI319_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY6864037.1"
                     /translation="MEERVDFVSGGLRLAGILHLPERRRGRVPAFLVLHGFGSNKDGG
                     TAMIAAKLFAEMGYAALRFDMRGCGESEGERGRVICLEQVEDTRSA"
BASE COUNT          742 a         1585 c         1585 g          612 t
ORIGIN      
        1 gcggcacgac ggccagcacc gagagccgga cgcgcttgag ccgcaacgac accgacacga
       61 ccggcagctc gacgtcgaac gtgaacccgt cgcgctccac gcgcggcgcg gacaaccacg
      121 cggtcaccgg cagcgccggg acgtcgggcg ccgccaacgt gactcagtcc gacatctcga
      181 atgcacccgg caccccgggc agcagctcgc cgtcgcgctg atctcgacgc aacccaagga
      241 gactccatgc aagcaaagac tctcatcgcc gtcctcgtcg cgggcctgtt cgccgccccg
      301 atcgcgttcg cccagaccgg cggcaccggg ccgagcggaa tggaccagcg ctcggcggac
      361 gagcagacgc cgcccaaggc cgaccaggag aagagcacca ccggccgcag caccgcgccg
      421 aaggcgaaga cgagcaagcg cagcagcaag cgcgaccacg agcgcctgag ccgcaacgac
      481 cgtggcacgg ccgcggacac gcccaacatg gcgcgcagcc cgagaacttc cggcaatccg
      541 ccgaagcagg gcagcagccc cgagccgcag tagaccgcgg catcgcgagg cgtcccgcgt
      601 ccggctcgcc gggcgcggga tttttttcgc ccgcgtcttt ggcaggcacg gagcgcctcc
      661 tgtaggatgc agccggaagg aggcgggcgc gaatgaagat ggcgaaggcg gtgctggcgg
      721 ccgcggcgct gtgtctggcg ggtgccgcgt gcgcgcagag cgccgcgagc gggtcgggct
      781 acccggtcaa agccatcaag ctgctggtcg gttttccgcc cggcagcgcg gccgacgtgg
      841 tcgcgcgcat cgtgtcgccg cggctcggcg agacgctcgg tcagccgctc gtcgtcgaga
      901 accgggccgg tgcgagctcg agcatcgcgg cggaggccgt ggtgcgatcg cccgccgacg
      961 gctatgcgct cttcttcggc tcggtcgcga acgcgatcaa cgcgagcctc aacaaggacc
     1021 tcagcttcga tttcgtcaag gacctcgcgc cggtcgcggg actgacgacg atgccgaacc
     1081 tgctcgtcgt gcacccgtcg ctcgaggtgc gctcggtggc cgagctgatc gccgcggcga
     1141 gagcgaagcc ggatgccatc ctctacggct cgtcgggaaa cggcacctcg ccgcacctct
     1201 cgggcgagct gttcaacctc gcggccggcg tgaagatggt gcacatcccg tacaagggca
     1261 gcccgcaggc cgtcaccgac ctgctcgccg gccgcgtgca ggtgatgttc tcgccggcgt
     1321 cgacggttct cgctcacatc aaggcgggga agctgcgcgc gctcgcgtcg acgggcgcgc
     1381 agcgcaccgc ggcggctccc gacctgccga cggtctcgga gtcggggctg ccgggattcg
     1441 agacctcgat ctggttcggg ctgttcgcgc cggcggcgac gccgcgcgac gtgatcgacc
     1501 ggatcgcgcg cgagacggcg cgggtgctcg ccgcgcccga cgtccgtgcg cagctcgcga
     1561 ggcagggcgt ggaccccatg ccgaagacgc ccgagcagct cggcgaatac gtgcgcgacg
     1621 aggtcgataa atgggggcag gtcgtccgca acgccggcgt tcgcgccgac tgagctcggg
     1681 aaaaaaaagc gcatgaaccc gttcgttcgc ttcgtcgtcg ccgacgcgct cgcgtcggcg
     1741 ccgctcgtcg cgctcgcgca ggactatccc gccaagccga tcaagctgct gatcgggttt
     1801 ccgcccggcg gcaacgtcga cgtcgtcggg cgcatcgtcg cggcgaagat gagcgagggc
     1861 ctcgggcagc aggtcgtgcc cgagaaccgc accggcgcgg gcggcatcat cgccaacgac
     1921 gccgtcgcca aggcggcgcc cgacggctac acgctgctcc tcgtgagcgg cgcgcacgtg
     1981 acccaggccg cggtgcgcgc ctcgcttccc tacgacccgg tgcgcgcgtt cgcctgggtc
     2041 tcgacggtgg tgaagtatcc gctcgtgatc gaggtgcgct cggattccag cttcaaatcg
     2101 ctcgacgatt tcatcgccta cgtgaagggc aatcccggca aggtgtacta cccgtcgccg
     2161 ggcatgggca ctctctatca cctcgccgcc gagcagttca tcgcgatggc cgggatcgag
     2221 atgcagcacg tgccgttccg cggcggggcc gagccgctga ccgaggtgct cgccgggcgc
     2281 atgccgatgc tgttcgacgc gctcaccaat tcgtatccgc agatccgcgc cggcaagttc
     2341 cgcccgctcg ccgtgacctc ggccgagccc tcggccgcgc tgcccggcgt gccgaccgcc
     2401 gcgcacagcg tcccgggcta cgaggcgtcg tcgttctcgg gcatcgcgac gacggcgggc
     2461 acaccgcccg cggtgatcga gcgcctgaac cgcgagctgc gccgggtgtg cgcgctgccc
     2521 gacgtcgcgc agcgcttcgc cgagtggggc gggcagcccg aggcgagcag ccccgaggag
     2581 atgcgcgcct acgtcgagcg cgagatcgcg aaatggaagc gcgtggtgga acagaggaag
     2641 atcgagctgc agtgaacaac ccccaaagga ggcgaccgtg aagaagagca ggctcgcaat
     2701 cgcagcagct ttcgccgccg gcgcggtgtt cggcgcgctc ggcatccagt ctctcaacgc
     2761 acagcagccc gggttcaagc gcgcgctgct gcagaagcac gaattgagcg cgccgggccg
     2821 cgacgccgtg gtcgcgaccg ccgagttcga gcccggcgtg gccgcggggc gccatacgca
     2881 tccgggcgag gagttgagct acgtcctcga gggcacggtg ctgctcgagg tcaccggcca
     2941 gccgccgcgg acgctgcacg cgggcgacgt gttcttcatc ccgccgaaca cgatccacga
     3001 cggcaagaac atcggcaaag ggccggcgaa ggtgctcgcg acctacatcg tggagcgcgg
     3061 caagccggtg gcgactccgg cgaaatagat ccaggacgcg cgtaccccgg ccgcgggggc
     3121 gcacgtctca tggcgcacgc ggcgatacgg attcatgcga ctcggctact tcaccatgcc
     3181 cctgcacccg ctgcaccggg atccggcgca gacgctgcag gaagaccgcg aggcgatcat
     3241 cctcgccgac cggctcggct tccacgacgc cttcgtcggc gagcacctca cggaccgcgc
     3301 cgagaacgtc accagcagct tcatcttcct cgccagcctg atcgccgaga cgaagacgat
     3361 ccggctggcg accgggacgt cgaacctctc gcaatcgcac ccggtggtga tcgcggcgca
     3421 ggccgcgatg ttcgatcacc tcgcgcgagg ccgcttcatc ttcggcgtca gcccgggcgc
     3481 gctgtcctcg gatgccgagg cgctcggcat cctcgagcag gaccgcaacc gcatgttcgc
     3541 cgagtgcatc gacgtgatcc tcgagatctg ggaacgcgat ccgccttacg acatcgagct
     3601 cgccgggaac cgctggaagg tgacgacggc gaaaacctac gtgcccgaca tcggccgcgg
     3661 cgtgatgtac aagccgtacc agaagccgcg gcccgagatc gtcggcacgg tggtcgcgcc
     3721 gttctcgaag ggcgtgatcg cgatgggcga gcgcgatttc cacccgctct cggcaaactt
     3781 cttgctgccg cactggctgc cgtcgcactg gacgaattac gtcgagggca agcgccgcgc
     3841 cggaaaggcc gccgatccgt ccgactggcg cgtcgcgcgc accatcttcg tcgccgacga
     3901 cgcgaagacg gcgcgccgct acgccgtcga agatccagcg agcccgtacg cgttctactg
     3961 gaagcagctg cgcaccaaga tgatgctcgc gaagcgccac gtgatcttca agatgcgcga
     4021 ggacgaggac gacgccgcgg tgacgctcga gcggctgctc gatcggctcg tgatccacgg
     4081 cacgcccgag caggtcgccg agcgcatcgt cgcgctgcgc gcgcaggccg ggccgttcgg
     4141 cgagatcgtc tacgcgggca tggactgggt cgacgccggg ctcgcgcggc gctcgatgga
     4201 gctgatggcg accgaggtga tgccgcgggt gaacgcggcg ctgaaagaag ggcaatggaa
     4261 gagcgcgtcg atttcgtctc cggcggcctg aggctcgcag gcatcctgca cctgccggag
     4321 cgccggcgcg gcagagtccc cgcatttctc gtgctgcacg gcttcggcag caacaaggat
     4381 ggcggcaccg cgatgatcgc ggcgaagctc ttcgcggaga tgggctacgc cgcgctgcgc
     4441 ttcgacatgc gcggctgcgg ggagagcgaa ggcgagcgcg ggcgggtcat ctgcctcgag
     4501 caggtcgagg acacgcgcag cgcg
//
LOCUS       DATDMW010000211        34810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1683728, whole genome shotgun sequence.
ACCESSION   DATDMW010000211 DATDMW010000000
VERSION     DATDMW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 34810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34810
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683728"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VI319_09065"
     CDS             <1..637
                     /locus_tag="VI319_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY6864038.1"
                     /translation="FVAIGLNYIDHAQEAGLQIPAEPVIFSKATTCISGPNDDIQQPR
                     NSTKLDYEVEIAIVIGTRCQYVPEDKALEYVAGYCVVNDVSERAFQLQSSQWDKGKGC
                     DTFGPIGPWLVTTDEITNPQTLDIWLDVNGERRQSSNTRNMIFSCARIVSYCSNYMTL
                     MPGDIITTGTPAGVALGMKPTQAWLKPGDIVTLGIKGLGEQRQKVVPYSGK"
     gene            816..2123
                     /locus_tag="VI319_09070"
     CDS             816..2123
                     /locus_tag="VI319_09070"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEY6864039.1"
                     /translation="MKIIVLGAGVIGTTSAWYLARAGHEVVVVDLRSQPGLETSFANG
                     GQISVSHSEPWANPTAPFQILRWLGREDAPLLFRPRLDRGQWLWGARFLRECLPARAR
                     RNTAQALALAIYSREQLQELRRETGIEYDAQTRGILHLYEERRELLHAQAQVDFLRAR
                     GLDVSLKSLEECVEIEPALRHAAHRFAGAAYARNDESGDASLFTRNLGELCRRQGVDF
                     RFNLTITRIECGEGRVARVVVDDEHGAEESLRADAYVVALGSYSPLVLRPIGIAVPVY
                     PVKGYSITLPLAAGDEAPVVSLTDHSRKLVFSRLGERLRVAGTAELNGYDSEINAVRC
                     RALVERCFELFPRAGRPGDAQYWTGLRPATPSNLPIVGRSRIANLYLNTGHGTLGWTL
                     SCGSGRALADIVGGRRPEPEFGFLGLEPKKRGAAIPAAPRLSA"
     gene            complement(2130..3431)
                     /gene="speE"
                     /locus_tag="VI319_09075"
     CDS             complement(2130..3431)
                     /gene="speE"
                     /locus_tag="VI319_09075"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEY6864040.1"
                     /translation="MRRCISVIKQSGDVCKLDGLHVVANLYRCRGSARYLTDAAELRR
                     FCHDSVNRSGLTILGDLFHQFDGGGVTGTVVLAESHLAIHTWPELQSVTLDVYVCNYT
                     QDNTEKARKVVADLMEVFGPEEHVQHDVPRDKQFMYEWLNGDYGFFLRSSKRIETCRT
                     RFQDLEIHESPQFGKLFRLDGCFMTSEKEEFVYHENLIHPGLTAHPEPKQVLIIGGGD
                     GGAAEEALKHPSVERVTLVELDEKVVDIAREHFGEIHKGVFDNPKLRLLIEDGLKFLA
                     TTEERFDVVALDLPDPIGPATALYEEAFFRDCRRSLAPGGVLTLHMGSPVSRPDRVRE
                     IYQRLGNVFPIVRPYTMFIPLYGCLWSMACCSQTLDVSTVTPSEIDRRIARRALPHLQ
                     YYNGATHQAVFALPNFVRDLTVGVRPTPKLVPKRRAAAGGK"
     gene            complement(3441..4268)
                     /gene="nudC"
                     /locus_tag="VI319_09080"
     CDS             complement(3441..4268)
                     /gene="nudC"
                     /locus_tag="VI319_09080"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY6864041.1"
                     /translation="MPFQRPADYTPAIAAPPQPPEHAFWFVFRGSELLVAAPRPRLPH
                     CNLPKELGMMPSRTQYLGVWGGEHCYSAEVAQEAQAPQGWSWQGLRSLFSVLDDGAFA
                     LAGNALQIMDWDRTHQYCGACGAATTARTNERSRECPRCGLVVYPRLAPAVMALVRRG
                     REILLARSPRFPQGMYSALAGFVEPGETIEQCLEREVLEEVGIRVRDARYFASQPWPF
                     PHSLMIAFHAEHEAGELRVDGVEIEDAKWFQLCNLPKLPARISIARRLIDAAVAEAR"
     gene            complement(4298..5176)
                     /locus_tag="VI319_09085"
     CDS             complement(4298..5176)
                     /locus_tag="VI319_09085"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6864042.1"
                     /translation="MSKQKLAFIGLGVMGFPMAGHLVQKGGHEVTVYNRTRAKADAWA
                     AKHGGKVAATPAKAAEGAGIVFSCVGDDPDLREIALGRDGAFAAMARGAVFVDHTTAS
                     AKVARELAAEAKKRGFEFLDAPVSGGQAGAENGQLTVMVGGDGAAFERAKPVIAHFAK
                     AVTLMGPSGSGQLTKMVNQICIAGLVQGLSEGLNFAMKAGLDAKLVLDVISKGAAQSW
                     QMENRGKTMVDDKFDFGFAVDWMRKDLRICAEEARANGAPLPVTQTVAGFYDEVSKMG
                     GGRWDTSSLIRRLRRS"
     gene            5318..7396
                     /gene="tkt"
                     /locus_tag="VI319_09090"
     CDS             5318..7396
                     /gene="tkt"
                     /locus_tag="VI319_09090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY6864043.1"
                     /translation="MSPDASASGHRDPAPDLVDVAKSGGAERELANAIRVLAMDAVQA
                     ANSGHPGMPMGMAEIAVALWRRHLRHNPENPRWHDRDRFVLSNGHGSMLLYALLHLTG
                     YDLPIEELKRFRQLHSKTPGHPEYGITPGAETTTGPLGQGIANAVGMALAERLLAAEF
                     NRPGHDIVDHHTYVFMGDGCMMEGISHEVCSLAGTLKLGKLIALYDDNGISIDGHVRG
                     WFTDDTPRRFEAYGWHVVPNVDGHDVEAVDRAIRAAKAVGDKPSLICCKTVIAHGAPK
                     KAGTHDSHGAPLGADEVAATRAALGWPHAPFEIPRRVYDGWDARARGATLEQAWLERF
                     ARYAKAHPELAREFERRMAGALPQAWAESARQIVAKAEDKAESVATRKASQNALEAIG
                     PVLPELVGGSADLTGSNLTMWSGSRPVGPQGREAHGGNYLYYGVREFGMSAVMNGLAL
                     HGGFIPYGGTFLTFSDYSRNALRMAALMRVRSVFVFSHDSIGLGEDGPTHQSVEHAAA
                     LRLIPGFDVWRPCDTVETTVAWIAAIGRGDGPSALLLSRQNLPFQKRSAAVVAQIARG
                     GYVLADADGARAVILATGSEVALAMGAKVKLAEQGIAARVVSMPSTSVFDRQDAAWRE
                     SVLPAGLPRVAVEAGVSDFWRKYVGAVDSRRGAVVGIDRFGESAPGPRLFEHFGFTVD
                     NVVKAVKSVL"
     gene            7426..8442
                     /gene="gap"
                     /locus_tag="VI319_09095"
     CDS             7426..8442
                     /gene="gap"
                     /locus_tag="VI319_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY6864044.1"
                     /translation="MTIRVAINGYGRIGRNILRAFYESGRKHDLQFVAVNDLGNAQTN
                     AHLTRYDTVHGKFPGKIAVDGDALVVNDDRIQVCAKRNPAELPWKDLKVDVVLECTGL
                     FTSKEKASAHLAAGAKKVIISAPGGKDVDATIVVGVNHKTLKASHTVISNASCTTNCL
                     APLVKALHDRIGVVCGLMTTIHAYTNDQVLTDVYHEDLRRARSATQSQIPTKTGAAAA
                     VGLVLPELNGKLDGYAIRVPTINVSIVDLSFVAKRPTTVDEVNKAVKEASETDLKGIL
                     EYNQDPLVSIDFNHNPASSIFDSTLTKVSEGTLVKVSSWYDNEWGFSNRMLDATVALM
                     NARS"
     gene            8476..9651
                     /locus_tag="VI319_09100"
     CDS             8476..9651
                     /locus_tag="VI319_09100"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY6864045.1"
                     /translation="MSEVDLRGKRVLVRVDFNVPIQDGRITDDTRIRAALPGIRHALG
                     QRARLMLVSHLGRPTEGQFDEKESLAPVARHLSGLLGMEVPLKRDWLGGVDVEPGKAV
                     LLENCRFNKGEKKDDDALAKKMASLCDVYVNDAFGTAHRAEATTHGIAKYAPVACAGP
                     LMAAEIDALSKALERPARPLVAIVAGSKVSTKLSILASLADKVDSLIPGGGIANTFLL
                     ASGREIGKSLAEPDLVPEARKITELMRKKGGEVPLPVDVVCAKEFAGGAHAEVKALGE
                     VADDDMILDIGPKSAAEFARVLSRAGTIVWNGPVGVFEFDQFGQGTKALAQAIAGSEA
                     FSLAGGGETLAAIAKYGVGDKISYVSTGGGAFLEFLEGKKLPAIEILEQRAAKAPAR"
     gene            complement(9669..10793)
                     /locus_tag="VI319_09105"
     CDS             complement(9669..10793)
                     /locus_tag="VI319_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEY6864046.1"
                     /translation="MLATQIRQKDGIFYFGSFRAPELLQRVRFISRFYGEGEEIAPAR
                     ISHDDEIAQFISKIERTDKAFQRTLSRAKVKQLKNFYETAVTQPPIPGTVLLFTPEKL
                     RFTPAGSGESVGTLSEPQQKFLIIDGQHRLAALRFYMEERPDDAKTINVPCVIFDGRS
                     EDFATEMFVIINSTPTRINKSHLVDLYERVSWAAPDRKFAARMVSGLYSEGDSPLRYR
                     INRLGGRSQQDKWILQAELFNELHRWVSRDWRKIQVAGGSAKEAGRYYAIVRDFLKAS
                     EKVWGEAWGNPNYMVTRPVTLKALIRVCADLARENADPAEGRIKRWEERLSPWSEQLR
                     AFKVEGFYERFAAKGEVERVSRVHRDLAKLAGIEVRTAKA"
     gene            10990..12429
                     /gene="pyk"
                     /locus_tag="VI319_09110"
     CDS             10990..12429
                     /gene="pyk"
                     /locus_tag="VI319_09110"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY6864047.1"
                     /translation="MQRSTKIVATLGPASSDAATLERMFLAGVDVVRLNFSHGTAEDH
                     VRRAEMVREASRKTGRTVGIMADLQGPKIRIGKFKDGPITLKPGDSFILDADCELGDD
                     RRVGLDYKELPRDVHPGDMLLLDDGRIVFEVDKVLGNEIHARVEHGGVLSNNKGINRL
                     GGGLTAPALAAKDMEDIRTAAKIRVDYLAVSFPKSSADMYMARQLMRAAGGDAFLIAK
                     IERAEAVEPRALEDIMSACEGIMVARGDLAVEVGDASVPALQKRMIRMAREQNKITIT
                     ATQMMESMINSPVPTRAEVSDVANAVLDGTDAVMTSAETASGKYPVETITNMARICSV
                     AEQSQPVTLDRDFLDRTFTRVDQSIAMAALFTAYHLKVKAIAALTESGSTALWMSRLN
                     CGVPIFALTSQTSTRYRCALFRDVYPLMVKYVGHDREELLAEAENVLVKNDVVQAGDL
                     IVLTIGEPIGKAGGTNTMKIVRVGEHRRG"
     gene            12467..13624
                     /locus_tag="VI319_09115"
     CDS             12467..13624
                     /locus_tag="VI319_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6864048.1"
                     /translation="MGEKRLGIIMHGVTGRMGTNQHLERSIVAIRKQGGVRLSDGTRV
                     MPDPVLVGRNAAKLQALASRHGIERWTTDIEVALANEGDAVFFDAASTQLRPALLRRA
                     IARGKHVYCEKPIATTLAQALGVARAARKARVRHGVVQDKLWLPGLLKLQKLRDSGFF
                     GRMLSVRGEFGYWVFEGDRREQPAQRPSWNYRKPDGGGIILDMLCHWRYVLDNLFGEV
                     RRVSCLGATHIPARWDETGRKYRVTADDAAYATFELRGGVIAHVNSSWAARVRRDDLA
                     VFQVDGTHGSAVAGLTRCFAQSLNATPRPVWNPDVRQAHDFLADWQEVPDDGVYENAF
                     KAEWELFIRHVCEGAPFRWNLVEGAKGLQLVDCALRSWKYRRWIDVPKLKI"
     gene            13633..14823
                     /locus_tag="VI319_09120"
     CDS             13633..14823
                     /locus_tag="VI319_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY6864049.1"
                     /translation="MNAPEKPKLKIRLPAGGRRLEEYRLRGPRDFAMLGKPKFDRIAL
                     AAAHVVGDPCSARDPWLEAAVDWDATLAYRRYLWSLGLGVAEAMDTAQRGMGMDWSAS
                     LELVRRSIDASRDVEGAVLFSGAGTDQLTPAAAVKPADVLAAYEEQCGAIEALGGRIV
                     LMASRALAACARGADDYLEVYARVLSQVREPVIIHWLGDMFDPALAGYWGSRDPAAAM
                     ETALAVVSANAAKVDGIKISLLDKDKEIAMRRRLPAGVRMYTGDDFNYPELIEGDAEG
                     HSDALLGILDAIAPAASAALAWLAAGDREGFRSLLAPTLPLSRHVFSAPTRFYKTGIV
                     FLAWLNGHQDHFVMVGGQQSARSIAHLAETFRLADAAGLLRDPELAVARMKRLLAVHG
                     LDAR"
     gene            14820..15674
                     /locus_tag="VI319_09125"
     CDS             14820..15674
                     /locus_tag="VI319_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY6864050.1"
                     /translation="MTRPGPPDAGLLSLNTATVREKWSLREIIAGCARHRIPGISPWR
                     DQLAQLGAKEAAKVIRDHGLRVTGLCRGGMFPAADRKGRRAAIDDNLRAIDDAAVLEA
                     ACLVLVVGGLPAGSRDLAGAREMVRDGIGEILDHARSAGVPLAIEPMHPMYCADRGCV
                     NTLAQALDLCDQFGSGSSRGDLGVVVDVYHVWWDPDLKAQIERAGDRRLLAYHICDWL
                     VPTTDLLLDRGMMGDGVIDLPLVRSWMSEAGYRGFHEVEIFSARNWWKRDPDEVLMRC
                     RERHQIAS"
     gene            15794..16819
                     /locus_tag="VI319_09130"
     CDS             15794..16819
                     /locus_tag="VI319_09130"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY6864051.1"
                     /translation="MDTNLLSKTAAAMVAKGKGLLAADESSGTCEKRFKSVGVESTES
                     NRRAYRDLLFTTKGIEDYVSGVILFDETLRQSTVSGNDPFPQYLQKKGVIPGIKVDKG
                     THNLALAPGEVVTEGLDGLRGRLEEYFKLGARFAKWRAVINIGPGIPTHACLYANAHA
                     LARYSALCQEQSIVPIVEPEVLLDGNHTIERCFEATEATLAALFAALRAHNVSLEHTV
                     LKASMVVPGKDGGRQASVDEVAELTVRCLKRTVPAALPGVVFLSGGQSDEAATAHLNA
                     MAQMPNLPWPLTFSYSRALQNPALKTWCGKAGNVAAAQRAFYHRARMNSLAAQGKYRP
                     ELEKQAA"
     gene            16819..17724
                     /locus_tag="VI319_09135"
     CDS             16819..17724
                     /locus_tag="VI319_09135"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY6864052.1"
                     /translation="MSASAPLYESGLQSLPLLGRGKVRDMYAVGSDRLLIVTTDRLSA
                     FDVVLQDPIPDKGRVLNAMSNFWFAKLAHIIPNHLNGASPESVVKGEREAVQVRGRSV
                     VVRKLKPLPIEAVVRGYLIGSGWKDYQATGGVCGIPLPKGLKQADKLPEVIFTPATKA
                     EIGAHDENISFEQVEKTIGRDLAKQVRDVSIRLYREAADYARERGIIIADTKFEFGLD
                     DAGRLVLIDEALTADSSRFWPADQYRPGSSPPSYDKQYVRDYLETLDWNKKPPAPRLP
                     PDVLARTSEKYREALYRLTGQKLGG"
     gene            17729..18223
                     /gene="purE"
                     /locus_tag="VI319_09140"
     CDS             17729..18223
                     /gene="purE"
                     /locus_tag="VI319_09140"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY6864053.1"
                     /translation="MASSTPLVGIVMGSTSDWEVMQQAAKQLADFSVPYEARVLSAHR
                     TPEALEQWLDDLTAKGAKCFIAGAGGAAHLAGVVAAKTTLPVLGVPMPSKHLQGLDSL
                     LSIVQMPKGIPVATFAIGDAGAANAGLFAVAMLALADEKLAKRLQDFRRAQADAVKNA
                     RLPA"
     gene            18243..19181
                     /locus_tag="VI319_09145"
     CDS             18243..19181
                     /locus_tag="VI319_09145"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY6864054.1"
                     /translation="MASEPVARAVEILGRGGLVAFPTETVYGLGGDASNDAAVARIYR
                     VKGRPADHPLIVHIGDASLLSRWARDVSADARKLAERFWPGPLTLVLKRAATVSDRVT
                     GGQDTVGLRVPGHPLALELLRAFGGGIAAPSANRFGKLSPTTADHVRRDLGSDVDLVL
                     DGGPCEVGIESTIVDLSRGAPVLLRPGRIGAADIAAAIGHDPRPRDAAAPRAPGALEV
                     HYAPRHPLRLVSRSELEPAVTRGSAVLAFEEAPASTAATMWTRASSDPQRYAHDLYAL
                     LRRLDEAAAATILVERPPEGPGWEAVLDRLTRAAGR"
     gene            19278..19353
                     /locus_tag="VI319_09150"
     tRNA            19278..19353
                     /locus_tag="VI319_09150"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19311..19313,aa:Ala,seq:cgc)
     gene            19466..20635
                     /locus_tag="VI319_09155"
     CDS             19466..20635
                     /locus_tag="VI319_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864055.1"
                     /translation="MAGKLRVLLVEDSEDDAALVLAELKRAGYELLSERVYSAQALQD
                     ALSRREWDFVIADYSMPSFTGLEALKMVRGSGRDVPFIVVSGTIGEDVAVDAVKAGAN
                     DYLIKDRLARLAPAVERELREATERRDRRSAESALRESEDRYRGALQEANLRLQSLSS
                     RMLEVQESERRHIARELHDEIGQALTAVKLHLQSAMRRCAPEARPPLEECVLITDQAL
                     DQVRNLSLNLRPSQLDDLGLIAALRWQIGRQAAASGLHTEFEADELSERLEPALETAC
                     FRFVQEAMTNVARHAQASNAWIEARRKGDRLRVSVRDDGRGFDVAEATREAIARGSLG
                     LLGMQERVGLAGGRLNVSSRPGEGTTIVAEFPLRFAPPRARRARRNDNAGSSTAR"
     gene            20610..21260
                     /locus_tag="VI319_09160"
     CDS             20610..21260
                     /locus_tag="VI319_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6864056.1"
                     /translation="MPVRVLLVDDHTLVRAGIRSLLDGMEGFTVVGEASDGREALAQL
                     KAQQPDVVLMDIAMKDLNGLETTIQIKRDFPGTRVVILSMHGTGDYVQQAIRAGASGY
                     LMKDAATAELGIALQAVMRGEVYLSPAVSKQVVESYMLRPGGDKHPAEQLTPRQREVL
                     QMIAEGLSTKEIAYRMGVSVKTVETHRTQLMERLEIRDVAGLVRYAIRIGLISAEK"
     gene            21436..21810
                     /locus_tag="VI319_09165"
     CDS             21436..21810
                     /locus_tag="VI319_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864057.1"
                     /translation="MKVLLVDDSPYFIRAASRFLSELEAAVAVTTAGNGTDAVKAVLS
                     DRPDLIIMDINMPGTGGLAAVRHIKAIEPDVRVVVVSLNESDDFRAAARAAGADGYIA
                     KRHFATGIVAFFASELDPPRPA"
     gene            complement(21815..23779)
                     /locus_tag="VI319_09170"
     CDS             complement(21815..23779)
                     /locus_tag="VI319_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY6864058.1"
                     /translation="MPLAALRRKLFGKPLDPLDTRTRHNIALVAFFAWVGLGADGISS
                     SCYGPEEAFRALGSHTHLGLYLALAVAVTVFIIALAYNQVIELFPTGGGGYRVSTRLL
                     GPYAGLVSGAALILDYVLTIAISIASGVDALFSLLPLSFQPYKLITEGTLILVLTGLN
                     LRGMREAITVLLPVFVGFIVTHAFLIVYGVLTHAPELPTLVPETLSETHDLASQLGWT
                     GVAALLLLAYSQGGGTYTGLEAVSNNVNVLAEPRVHTGKVTMLYMAISLAFTAGGIVL
                     LYLLWDAKPVEGQTLNAVAFGSIIQSFGWSSPWMNQAGLIVVLALEAGLLFVAANTGF
                     LGGPAVLANMSFDSWVPHKFRYLSARLVTENGILVMGVAALGILLWTGGSVSTLVVLY
                     SISVFLTFATSLFGLCVYWGWHRRERHWLRRLLLSAAGFVVCAGILLMLMAQKFLEGG
                     WLTALIIGAIVGVCIAIRMHYNWTRQQIRKLDAQYADLPFGSVATAPKPDPGQPTAVF
                     LVGSSRGGGMHALLWVQRMFPNHFRNFIFVNARAVDSQSFGGRQQMDAMEVEAKSALS
                     YFVNYCNSKGWAAKSYLSFGTDPIDEFTRLAQRVHAEFPNSIFFTSKLVFDNENWFVR
                     LLHNQAATVLQRRFHLRGMQMVILPMKLSV"
     gene            complement(23781..24122)
                     /locus_tag="VI319_09175"
     CDS             complement(23781..24122)
                     /locus_tag="VI319_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6864059.1"
                     /translation="MTSPVIANLQKLIGTPRDGALLRYSLGNEYLKAGDADNAEAQLR
                     EAVNRDPGYSAAWKLLGKALADSGRLADALAAYREGIAVAERKGDKQAAKEMTVFSRR
                     IERQLDAGRHR"
     gene            24128..26278
                     /gene="recG"
                     /locus_tag="VI319_09180"
     CDS             24128..26278
                     /gene="recG"
                     /locus_tag="VI319_09180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY6864060.1"
                     /translation="MGPDRPLRSDYKSAVPGSLSARPALISCPLSKSARNAADAHVRP
                     TSREASSAAKRLERLGIRREFDLVLHLPLRYEDETRITPIAGAPAGAAVQVEGVVRST
                     DIAYRPKRQLVCRVADESGEVVFRFFRFWASQVKALAPGVRVRAFGELRSGFFGGEMI
                     HPRFRVLRGESPLPAALTPVYPTTAGLSQLELRRAIETALARAQLDDTLPEALLARVG
                     LCGFREAIDALHHPLPGADSHDLLARAEPAWRRVKFDELLAQQLSMRKHYRERKARAA
                     RALAPKRTHTAALIERLPFRLTGAQGRAWSEIERDLAQPHPMHRLLQGDVGSGKTVVA
                     VLAALRAVEHGLQAAVMAPTEILAEQHYRKFSEWLVPLGLPVAWLSGAQSAKERKAAL
                     AAAASGEAPIVVGTHALIQEKARLRALGLAIVDEQHRFGVEQRLALRAKGAEPHVLMM
                     SATPIPRTLAMSFYADLDVSVIDELPPGRRPVITKLIADSRRAEVVQRIRAACRSGRQ
                     AYWICPLIEESEQLQLQTVLETHAVLGAELSDLRVGLLHGKLKPAEKAETMAAFVSGE
                     IQLLVATTVVEVGVDVPNASLMVIEHAERFGLAQLHQLRGRVGRGAEQSVCVLLFQNP
                     PGNAARQRLRVIYENTDGFAIAREDLALRGPGEVLGSRQSGAPLLRFADIVADAELLE
                     LASAAAERLLDEHPEAAQRHLDRWLGGRVEFLRA"
     gene            26307..27203
                     /gene="ubiA"
                     /locus_tag="VI319_09185"
     CDS             26307..27203
                     /gene="ubiA"
                     /locus_tag="VI319_09185"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY6864061.1"
                     /translation="MSLRAITDRISLYERLMRLDKPIGTLLLLWPTLWALWFASAGRP
                     DAWIVWIFILGTLLMRSAGCVLNDIADWQYDAKVKRTRERPLATGALGRGEALALAAV
                     LSLVAFGLVLTLDRLAIILSFVALGLAATYPLTKRFLAVPQAYLGVAFGFGIPMGYAA
                     VLGIVPAVGWMLLLANIFWAIAYDTEYAMVDRDDDVRIGIKTAAITFGRFDVAAVMIC
                     YAATLLVLAGIGAHYRMGPLYYGGLAVAGALMLYHYRLVRTRDRDACFRAFNQNNWVG
                     AAVFAGIFADFATKGLYPWISV"
     gene            27188..27976
                     /locus_tag="VI319_09190"
     CDS             27188..27976
                     /locus_tag="VI319_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6864062.1"
                     /translation="MDLGLKGRVVLVTGGSKGIGLACAGAFAAEGARVAIASRSRDNL
                     AAAEAKLRAAGHDATSIAADLGNPRGAMAAVAETERRFGPIDVLVNSAGAARRHSPEE
                     LDMQAWHEAMDAKYFTYVHAMQAVLPGMAARGRGAIVNIVGQGGKIASPAHLAGGAAN
                     AALMLLSAGLAAAYGKKGVRVNAINPGVTLTERVTGRFEAEGKTAGISPDEARRRGEQ
                     SIPLGRYGTPDEIAAVAVFLASDRASYVTGANIPMDGGAAATVV"
     gene            27976..29454
                     /gene="ubiD"
                     /locus_tag="VI319_09195"
     CDS             27976..29454
                     /gene="ubiD"
                     /locus_tag="VI319_09195"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEY6864063.1"
                     /translation="MADRDLRAFLARLEALGEAKRVRAEVDPRLEMTEICDRVLRAGG
                     PALVFERPSGHSIPVVANLFGTVRRVALAMGADASADPVASLREIGTLLAFLKEPEPP
                     KGVRDALERWLPIGRHVMHMAPKEVRSPPCQEIVREGADVDLTRLPLQTCWPGDAGPL
                     VTWGLTVTRGPHKPRQNIGVYRQQLVARDKLIMRWLAHRGGALDFADHCAAHPGEPFP
                     VAVALGADPATILAAVTPVPDTLSEYEFAGLLRGAKTETAKCLAGDLHVPAASEIVLE
                     GEIRPGETAEEGPFGDHTGYYNERERFPVFTVRRITTRRDPLYHSTYTGRPPDEPAVL
                     GAALNEIFVPILQKQFPEIVDFYLPPEACSYRLAVVSIRKQYPGHAKRVMFGVWSALR
                     QFMYTKFIVVVDDDVDVRDWKQVIWAVATRVDAARDATIIENTPIDYLDFASPAAGLG
                     SKLGIDATNKGPAETARAWGRPIAMDESVKRRVDAMWKDLGL"
     gene            complement(29501..30130)
                     /locus_tag="VI319_09200"
     CDS             complement(29501..30130)
                     /locus_tag="VI319_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864064.1"
                     /translation="MLAGSSSFSYAQDRNVLEGAYVGLGAGATRARFTQEDFATGVPG
                     IQESKDSSDKGYKLTFGAKLSRYWALELGRTNFGKFSHTYDGTGSGLGSVVETYKSAG
                     WWGSVLAVLPIGNSFSLFGRLGQMKSVTTTESINATGTITDTVAGSGVPVSGTKSKIS
                     TILGVGMQIDFPGRLGLRIEAEDYGTVGDSDNTGRARMRMATASLMYRF"
     gene            complement(30269..31792)
                     /gene="dacB"
                     /locus_tag="VI319_09205"
     CDS             complement(30269..31792)
                     /gene="dacB"
                     /locus_tag="VI319_09205"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY6864065.1"
                     /translation="MRRSGTGRALARSFILAFLALAPCLAGAAEPARLPATLRKALAA
                     AGIPIGATAVYVQDVAARTPMLSYNASRPMNPASSAKLVTTYAALEMLGPSFTWKTQA
                     YAGGPMRGDVLDGDLVLRGTGDPKLTIENFWLLLRALRERGLREIRGDLVLDHSYFAP
                     APYDPGKFDNEPARAYNVGPDALLVNFNAIRFHFGPDLEAKSLRVIPEPRPAQIELRA
                     SVALSDAPCGDWRSQIRAEFDSNDASARVNFSGTYPAACGENTWNVALLSHDSYLWGL
                     FRELWDELGGSFKGGVRDGTVAPDSRLIYSAESPPLSEVVRDINKFSNNVMARQLFLT
                     LSAEALHVPGDEHLSEGLIRDWALRKGIAADEIALENGAGLSRSDRISALTFGKLLVA
                     AWHSPVMSELVASMPVVASDGTMRRRLHGSDVAGQAHIKTGTLAGVRTLAGYVLARDG
                     RRFAVVFLVNHPNAAAAEAAQDQLLRWVYEAGRAVPREAGGGRRDVDRSEARSQMRE"
     gene            complement(31839..32165)
                     /locus_tag="VI319_09210"
     CDS             complement(31839..32165)
                     /locus_tag="VI319_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEY6864066.1"
                     /translation="MSDDAILEFLDEATEIAMDELLAASGLARAELIDLIELGVFDLR
                     PGARAADQASWRFSSRAIILGRRAARLRDDFGLNAPGMALALAYLERIEALELRLREL
                     ECQLLR"
     gene            complement(32162..33109)
                     /locus_tag="VI319_09215"
     CDS             complement(32162..33109)
                     /locus_tag="VI319_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY6864067.1"
                     /translation="MKYKDYYALLGVDRRASQDDIKKAYRRLARKYHPDVSKEPDAEE
                     RFKEVAEAYETLKDPEKRAAYDQLGSHQPGQDFRPPPDWETRFSPGGFEDIDLADLFA
                     HFGARPGRGFSGRAGADFAIAGEDYEVEARLSLEDAARGTELALQLAVPEMGPDGVRR
                     RMPKTVRVRVPKGATEGERLRIAGKGGPGVNGGRAGDLYLDIHLEPHPLFRVSGHDLY
                     LELPLAPWEAVLGTQVEIPTLDGRIELSVKPGAKAGQKLRVPGRGLPRPRGTSGDLYC
                     VLSIATPPEPGEREKSLYRELAAASHFDPRRHLKKGEPA"
     gene            33305..33601
                     /gene="groES"
                     /locus_tag="VI319_09220"
     CDS             33305..33601
                     /gene="groES"
                     /locus_tag="VI319_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY6864068.1"
                     /translation="MNKIRPLHDRVVVKRLEQEQKSAGGIVIPDNAAEKPDQGEVVAV
                     GNGKILEDGKVRPLDVKVGDRILFGKYSGQSVKVEGTEYLVMREDDIMGVVASK"
     gene            33633..>34810
                     /gene="groL"
                     /locus_tag="VI319_09225"
     CDS             33633..>34810
                     /gene="groL"
                     /locus_tag="VI319_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828028.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY6864069.1"
                     /translation="MPAKEVRFHEDARHKILAGVNVLANAVKATLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIFRE
                     GIKYVAAGMNPMDLKRGIDKGVAAMTEELKKISKPCTTSKEIAQVGSISANADSDIGK
                     IIADAMDKVGKEGVITVEDGKGLNNELEVVEGMQFDRGYLSPYFINSAEKQGCVLENP
                     YILLHDKKISSIRDLLPILEQVAKAGRPLLIIAEEVEGEALATLVVNTLRGILKTCAV
                     KAPGFGDRRKAMLEDIAILTGGQVIAEEVGLTLEKTTLKDLGQAKRVEVAKEETTIID
                     GAGDGKAIEARVKNIRTQIEEATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     "
BASE COUNT         5625 a        11880 c        12018 g         5287 t
ORIGIN      
        1 gttcgtcgcg atcgggctga actacatcga tcacgcgcag gaggccggcc tgcagatccc
       61 ggccgagccc gtgatcttct cgaaggcgac gacctgcatc agcggcccga acgacgacat
      121 ccagcagccg cgcaactcga cgaagctcga ctacgaggtc gagatcgcca tcgtcatcgg
      181 cacacggtgc cagtacgtac cggaggacaa ggcgctcgag tacgtcgccg gctactgcgt
      241 ggtgaacgac gtgtccgagc gcgccttcca gctgcagtcg agccagtggg acaagggcaa
      301 gggttgcgac accttcggac cgatcgggcc ctggctcgtc accaccgacg agatcacgaa
      361 tccgcagacg ctcgacatct ggctcgacgt caacggcgag cgacggcaat cgtcgaacac
      421 gaggaacatg atcttctcct gcgcgaggat cgtctcctac tgcagcaact acatgacgct
      481 gatgccgggc gacatcatca ccaccggcac gccggcgggc gtcgccctcg gcatgaagcc
      541 gacgcaggcc tggctcaagc cgggcgacat cgtgacgctc gggatcaagg ggctcggcga
      601 gcagcgccag aaggtcgtgc cctattcggg caagtaggcc cccgcaggcg aaagaaggcc
      661 gggtttcgcc cggctttctt tttccagctc tcgcgatcgg aacggtcgac cgcgtcgatt
      721 cgacggttgt ccaacgagac ggcgactgcg cgcagaactc gcttgccccg cgcggatcga
      781 actggaacaa tcccggcatt gggacgcaag ccgcgatgaa aatcatcgtt ctgggggccg
      841 gcgtgatcgg caccacgagc gcgtggtacc tggcgcgggc cggacacgag gtggtcgtgg
      901 tcgacctgcg gtcgcagcca ggcctcgaga ccagcttcgc caacggcggc cagatctcgg
      961 tcagccattc cgagccgtgg gcgaatccga cggcgccgtt ccagatcctg cgctggctgg
     1021 gccgcgagga cgcgccgctc ctgttccggc cgcggctcga ccgcgggcag tggctgtggg
     1081 gcgcgcgctt cctgcgcgaa tgcctgcctg cgcgcgcgcg ccggaacacc gcccaggcgc
     1141 tcgcgctcgc gatctacagc cgcgagcagc tccaggagct gcgccgcgag accggcatcg
     1201 agtacgacgc gcagacgcgc ggcatcctgc atctgtacga ggagcgccgg gagctgttgc
     1261 atgcgcaggc ccaggtcgat tttctgcgtg cgcgcggcct cgacgtgtcg ctgaagtcgc
     1321 tcgaggagtg cgtggagatc gagcccgcgc tccggcacgc cgcgcaccga ttcgcgggcg
     1381 ccgcctacgc gcgcaacgat gagtcgggcg atgcctcgct gttcacgcgt aacctcggcg
     1441 agctgtgccg ccggcaaggc gtcgacttcc gcttcaacct caccatcacg cgcatcgaat
     1501 gcggcgaggg acgcgtcgcc cgggtggtgg tcgacgacga gcacggcgcc gaggaatcgc
     1561 tccgcgccga cgcctacgtc gtggcgctgg ggagttacag cccgctcgtg cttcggccga
     1621 tcggcatcgc ggtgccggtc tacccggtga agggctattc gatcacgctg ccgcttgccg
     1681 ctggcgacga ggcgcccgtc gtcagcctga ccgaccattc gaggaagctc gtgttctcgc
     1741 gcctaggcga gcgtctgcgc gtggccggga cggcggagct gaacggctac gacagcgaga
     1801 tcaacgccgt ccgctgcagg gccctggtcg agcgatgctt cgagctcttt ccgcgcgcgg
     1861 gacgccccgg cgacgcccag tactggaccg gcttgaggcc cgcgacgccc agcaacctgc
     1921 cgatcgtcgg acgctcgcgc atcgcgaacc tgtatttgaa caccggccac gggacgctcg
     1981 gctggacgct ctcgtgcggg tcgggccgcg cgctggccga tatcgtcggc ggacgccgtc
     2041 cggagccgga gttcgggttc ctgggcctgg agcccaaaaa aaggggggcg gccatcccgg
     2101 ccgccccccg tctcagcgct tgagcggttc tacttgccgc cggccgccgc ccgccgcttc
     2161 gggacgagct tgggcgtagg ccgcacccct acggtcagat cgcgcacgaa gttggggagg
     2221 gcgaataccg cctggtgcgt ggcgccgttg tagtactgca ggtgcggcaa cgcgcggcgc
     2281 gcgatacgac ggtcgatctc ggagggcgtc acggtcgaga cgtcgagcgt ctgcgagcag
     2341 cacgccatcg accacaggca cccgtacagc gggatgaaca tggtgtacgg ccgcacgatc
     2401 gggaacacgt tgccgaggcg ctggtagatc tcgcgcacgc ggtccgggcg cgacacgggc
     2461 gagcccatgt gcagcgtcag cacgcctccc ggcgcgagcg agcgccggca atcgcggaag
     2521 aaggcctcct cgtagagcgc cgtcgccggc ccgatcgggt cgggcaggtc gagcgcgacc
     2581 acgtcgaagc gctcctcggt cgtcgcgagg aatttgaggc cgtcctcgat cagcaggcgc
     2641 agcttcgggt tgtcgaagac gcccttgtgg atctcgccga agtgctccct ggcgatgtcg
     2701 accactttct cgtcgagctc gaccagcgtg acgcgctcga ccgagggatg cttgagcgct
     2761 tcctcggccg cgccgccgtc gccgccgcca atgatcagca cctgcttggg ctcggggtgc
     2821 gcggtcagcc ccgggtggat caggttctcg tggtagacga actcttcctt ctcggaggtc
     2881 atgaagcagc cgtccaggcg gaagagcttg ccgaactgcg ggctctcatg gatctcgagg
     2941 tcctggaagc gtgtgcggca ggtctcgatg cgcttggagg agcgcaggaa gaagccgtag
     3001 tctccgttca gccactcgta catgaactgc ttgtcgcggg gaacgtcgtg ctgcacgtgc
     3061 tcctcgggac cgaacacctc catgaggtcg gcgacgacct tacgggcctt ctcggtgttg
     3121 tcctgggtgt agttgcagac gtagacgtcg agcgtgacgc tctggagctc gggccaggtg
     3181 tggatggcga gatgggattc cgccaggacc accgtacctg tcactccgcc accgtcgaac
     3241 tggtggaaca ggtcgcctaa tatggtcagg cccgacctgt ttacggagtc gtgacaaaaa
     3301 cgacgcagct cggctgcgtc agtcaagtac cgtgcggacc ccctgcaacg gtacagattg
     3361 gcgacaacat gcagtccgtc caatttgcat acatccccac tctgtttgat gaccgagatg
     3421 catctacgca tctcccccga ttaccgcgcc tctgccactg ccgcatcgat cagtctgcga
     3481 gcgatgctga ttctagcagg caatttcggc aaattacata gctgaaacca ctttgcgtct
     3541 tcgatttcga ctccgtcgac gcgcagctcg cccgcttcgt gctctgcatg gaacgcgatc
     3601 atcagcgagt gcgggaacgg ccacggctgg ctcgcgaaat agcgagcgtc gcgcacgcgg
     3661 atcccgacct cctccaacac ttcgcgctcc aggcactgct cgatcgtctc gcccggctcc
     3721 acgaagcctg ccagggcgct atacatgcct tgcgggaagc gcggcgagcg cgcgagaaga
     3781 atctcgcgcc cccggcgcac cagcgccatc accgccggcg ccagcctggg atacacgacc
     3841 aggccgcacc gcgggcactc gcggctgcgc tcgttcgtgc gcgccgtcgt cgccgccccg
     3901 cacgcgccgc aatactggtg cgtgcggtcc caatccatga tctgcagcgc gttgccggcg
     3961 agggcgaagg cgccgtcgtc gagcacgctg aacagcgacc gcagaccctg ccaggaccag
     4021 ccttggggcg cctgcgcctc ctgcgcgacc tcggcggagt agcagtgctc cccgccccag
     4081 accccaagat attgtgtccg agacggcatc atacccaatt ccttgggtaa gttgcaatgg
     4141 ggcaggcgcg gccgcggcgc ggcgaccagc agctcgctgc cgcggaagac gaaccagaac
     4201 gcgtgctccg gcggctgcgg tggagcggcg atggccggcg tgtaatccgc cggccgctgg
     4261 aacggcatgg acacggcgcg gcgcttccgc gtgaggctta ggaccggcgc aggcggcgga
     4321 tcaggctcga cgtgtcccag cggccgccgc ccatcttcga gacctcgtcg tagaagccgg
     4381 cgacggtctg ggtgacgggc aagggcgcgc cgttggcgcg cgcctcctcg gcgcagatgc
     4441 gcaggtcctt gcgcatccag tcgaccgcga acccgaagtc gaacttgtcg tcgaccatgg
     4501 tcttgccgcg gttctccatc tgccaggact gcgcggcgcc cttggagatg acgtcgagga
     4561 cgagcttcgc gtcgaggccc gccttcatcg cgaagttgag gccttccgac aggccctgga
     4621 ccagccccgc gatgcagatc tggttcacca tcttcgtgag ctggccggag cccgacgggc
     4681 ccatcagcgt caccgccttc gcgaaatgcg cgatgaccgg cttggcgcgc tcgaacgccg
     4741 cgccgtcgcc gcccaccatg accgtgagct ggccgttctc ggcacccgcc tgcccgcccg
     4801 agaccggcgc gtcgaggaac tcgaacccgc gcttcttcgc ctcggcggcg agctcgcgcg
     4861 ccaccttcgc cgaggcggtc gtgtggtcga cgaacaccgc gccccgggcc atcgccgcga
     4921 acgcgccgtc ccgaccgagc gcgatctcgc gcaggtccgg gtcgtcgccc acgcacgaga
     4981 acacgatgcc ggcgccttcg gccgccttcg ccggcgtggc cgcgaccttg ccgccgtgct
     5041 tggctgccca ggcgtcggct ttggcgcgcg tgcggttgta caccgtgacc tcatgaccgc
     5101 ctttctgcac gaggtgtccg gccatgggga agcccatcac gccgaggccg atgaacgcca
     5161 gtttttgttt gctcactgct cctccgggtt tttcggggtt ggacgcgagc gcgaaaggcc
     5221 cgccgggtgc gccgctctcg ccaagccggc gagattcgcc gataattata gggctcctca
     5281 tgtctttcca ccctttcttc ccggcctttt cccgaccatg agtccggacg cgagcgcctc
     5341 cggtcatcgg gaccctgcgc ccgacctggt cgacgtcgcg aagagcggcg gtgccgagcg
     5401 cgagctcgcg aacgcgattc gtgtcctcgc gatggacgcc gtgcaggcgg ccaactcggg
     5461 ccacccggga atgccgatgg gcatggccga gatcgccgtc gccctgtggc gccgccacct
     5521 gcgccacaac ccggagaacc cgcgctggca cgatcgcgac cgcttcgtgc tgtcgaacgg
     5581 ccacgggtcg atgctgctgt acgcgctcct gcatctcacg ggctacgacc tgccgatcga
     5641 ggagctcaag cgcttccggc agctgcactc gaagacgccc gggcacccgg agtacggcat
     5701 cacgccgggg gccgagacca ccacggggcc cctgggccag ggcatcgcca acgcggtcgg
     5761 catggcgctc gccgagcgcc tcctggccgc cgagttcaac cgcccaggac acgacatcgt
     5821 cgaccatcac acctacgtct tcatgggcga cggctgcatg atggagggca tctcgcacga
     5881 ggtgtgctcg ctcgcgggga cgctcaagct cggcaagctg atcgcgctct acgacgacaa
     5941 cggcatctcc atcgacggcc acgtgcgcgg ctggttcacc gacgacacgc cgaggcgctt
     6001 cgaggcgtat ggctggcacg tcgtgccgaa cgtcgacggg cacgacgtcg aggcggtcga
     6061 ccgcgcgatc cgtgcggcga aggcggtggg cgacaagccg agcctcatct gctgcaagac
     6121 cgtgatcgcg catggcgcac ccaagaaggc gggcacccac gactcgcacg gcgcgccgct
     6181 cggcgccgac gaggtcgcgg cgacgcgcgc ggcgctcggg tggccgcacg cgccgttcga
     6241 gatcccgcgg cgcgtctacg acggctggga cgcccgcgcg cgcggcgcga cgctcgagca
     6301 ggcctggttg gaacgcttcg cgcgctatgc gaaggcgcat cccgagctgg cgcgcgaatt
     6361 cgagcgccgc atggcgggcg ccctgccgca ggcctgggcc gaaagcgccc ggcagatcgt
     6421 cgccaaggcg gaggacaagg ccgagtcggt cgccacgcgc aaggcctcgc agaacgcgct
     6481 cgaggcgatc ggcccggtgc tgccggaact cgtcggcgga tcggccgatc taaccggctc
     6541 gaatctgacg atgtggtcgg ggtcccggcc cgtcggcccg caggggcggg aagcccacgg
     6601 gggcaactat ctctattacg gcgtgcgcga attcggcatg agcgcggtca tgaacgggtt
     6661 ggcgctgcac ggcggcttca tcccgtacgg cggaaccttc ctcaccttct ccgactactc
     6721 ccgcaacgcg ctgcgcatgg cggcgctcat gcgcgtgcgc tcggtgttcg tgttctcgca
     6781 cgactcgatc gggctcggcg aggacggccc gacgcaccag tcggtcgagc acgctgcggc
     6841 cctcaggctg atccccggct tcgacgtctg gcggccgtgc gacacggtcg aaacgacggt
     6901 cgcctggatc gccgcgatcg ggcgcggcga cggtccgtcg gcgctgctcc tgtcgcgcca
     6961 gaacctgccg ttccagaagc gcagcgcggc cgtcgtggcg cagatcgcgc gtggcggcta
     7021 cgtcctcgcc gacgccgacg gcgcgcgcgc ggtgatcctc gcgaccggct cggaagtggc
     7081 cctggccatg ggcgcgaagg tgaaactcgc ggagcaggga atcgcggcgc gcgtcgtatc
     7141 gatgccgtcc acgagcgtat tcgaccggca ggacgcggca tggcgcgaga gcgtgctgcc
     7201 ggccgggctg ccgcgggtcg cggtcgaggc gggcgtgtcc gacttctggc gcaagtacgt
     7261 cggcgcggtc gactcgcgcc gcggggcggt cgtcggcatc gaccgcttcg gcgagtcggc
     7321 gcccgggccc aggcttttcg agcacttcgg cttcaccgtc gacaacgtgg tgaaggcggt
     7381 caagagcgtc ctttagcttt agcggttctt cttcggagca cgtcaatgac cattcgggtg
     7441 gcaatcaacg gctacggccg catcggccgc aatatcctgc gcgcgttcta cgaaagcggc
     7501 cggaagcacg acctgcagtt cgtcgcggtg aacgacctgg gcaacgccca gaccaacgcg
     7561 cacctcacgc gctacgacac ggtgcacggg aagtttcccg gcaagatcgc cgtcgacggc
     7621 gacgccctgg tcgtcaacga cgaccgcatc caggtatgcg cgaagcgcaa tcccgccgag
     7681 ctgccgtgga aggacctgaa ggtcgacgtg gtgctcgagt gcacgggcct gttcacgagc
     7741 aaggagaagg cgtcggccca cctcgccgcc ggggcgaaga aggtcatcat ctccgcgccg
     7801 ggcggcaagg acgtggacgc caccatcgtg gtcggcgtca accacaagac gctgaaggca
     7861 tcgcacacgg tgatctccaa cgcgtcgtgc acgacgaact gcctcgcgcc gctggtgaag
     7921 gcgctgcacg accgcatcgg cgtcgtgtgc gggctcatga ccacgatcca cgcgtatacg
     7981 aacgaccagg tgctcaccga cgtctatcac gaggacctgc gccgcgcgcg ctcggcgacg
     8041 cagtcgcaga tcccgaccaa gacgggcgcg gcggccgcgg tcggcctggt gctgcccgag
     8101 ctgaacggca agctggacgg ctacgcgatc cgcgtgccga cgatcaacgt gtcgatcgtc
     8161 gacctctcgt tcgtggccaa gcgtccgacc acggtcgacg aggtcaacaa ggcggtgaag
     8221 gaggcctccg agaccgacct gaagggcatc ctcgagtaca accaggaccc gctggtctcg
     8281 atcgacttca accacaatcc cgcttcgtcg atcttcgact cgacgctgac caaggtgtcc
     8341 gagggcacgc tggtcaaggt gtcctcctgg tacgacaacg aatggggctt cagcaaccgc
     8401 atgctcgacg cgacggtcgc gctgatgaac gctcggtcct gaacgaagct gagggcgggc
     8461 gtggccatcc tgaagatgtc ggaggtggac ctgcgcggga aacgcgtgct ggtccgggtc
     8521 gacttcaacg tcccgatcca ggacggccgg atcaccgacg acacgcgcat ccgcgcggcg
     8581 ctccccggca tccgccacgc gctcggccag cgcgcgcgcc tcatgctggt ctcgcacctc
     8641 gggcgcccga ccgaggggca attcgacgag aaggagtcgc tcgcgccggt cgccaggcac
     8701 ctctcgggac tcctcgggat ggaagtcccg ctcaagcgcg actggctggg cggcgtcgac
     8761 gtggagccgg gcaaggcggt gctgctcgag aactgccgct tcaacaaggg cgagaagaag
     8821 gacgacgacg ccctcgcgaa gaagatggcc tcgctttgcg acgtctacgt gaacgacgcc
     8881 tttggcaccg cgcaccgcgc cgaggcgacc acgcatggca tcgcgaagta cgcgccggtc
     8941 gcctgcgccg gtccgttgat ggccgccgag atcgacgcgc tctcgaaggc gctcgagcgc
     9001 ccggcgcgac cgctggtcgc catcgtggcg ggctcgaagg tctcgaccaa gctcagcatc
     9061 ctcgcgtcgc tcgcggacaa ggtcgactcc ctgatccccg gcggggggat cgcgaacacc
     9121 ttcctgctcg cgtccgggcg cgagatcggc aagtcgctgg cggagcccga cctcgtgccc
     9181 gaggcgcgca agatcacgga gctgatgcga aagaaaggcg gcgaagtccc gctccccgtg
     9241 gacgtcgtct gcgccaagga gttcgcgggc ggggctcacg ccgaggtcaa ggcgctcggc
     9301 gaggtggccg acgacgacat gatcctcgac atcggcccga aatccgccgc cgaattcgcg
     9361 cgcgtgcttt cgcgggcggg cacgatcgta tggaacgggc cggtgggcgt gttcgagttc
     9421 gaccagttcg gccaggggac caaggcgctc gcgcaggcga tcgccgggtc cgaggcgttc
     9481 tccctggcgg gcggaggcga gacgctcgcc gccatcgcca aatacggcgt cggcgacaag
     9541 atctcctacg tgtcgacggg cggcggggcc ttcctcgagt tcctcgaggg caagaagctg
     9601 cccgccatcg aaatcctgga gcagcgcgcg gcaaaggcgc cggcgcgcta gcgccgcgcg
     9661 cgcccggcct aggccttggc cgtgcgtacc tcgatgccgg ccagcttggc gaggtcgcgg
     9721 tggacgcggc tcacgcgctc cacctccccc ttggcggcga accgctcgta gaaaccctcg
     9781 accttgaatg cccgcaactg ctccgaccag gggctgagcc gttcctccca gcgcttgatc
     9841 cggccttcgg ccggatcggc gttctcgcgc gcgaggtccg cgcacacccg gatcagggcc
     9901 ttcagggtga ccggccgggt caccatgtag ttgggattgc cccaggcctc gccccagacc
     9961 ttttcggagg ccttcaggaa gtcgcgcacg atcgcgtaat agcggccggc ctccttggcg
    10021 ctgcccccgg cgacctggat cttgcgccag tcgcggctga cccagcggtg cagctcgttg
    10081 aacagttccg cctggaggat ccacttgtcc tgctggctgc ggccgccgag gcggttgatg
    10141 cggtagcgca gcgggctgtc gccttcgctg tagaggccgc tcaccatgcg cgccgcgaac
    10201 ttgcggtcgg gcgccgccca cgacacgcgc tcgtacaggt cgaccaggtg gctcttgttg
    10261 atgcgggtcg gcgtcgagtt gatgatgacg aacatctcgg tcgcgaagtc ctcgctgcgg
    10321 ccgtcgaaga tcacgcacgg gacgttgatg gtcttggcat cgtcgggacg ttcttccatg
    10381 tagaaacgca gcgccgcgag gcggtgctgt ccgtcgatga tcaggaactt ctgctgcggc
    10441 tccgagaggg tcccgacgct ctcgccgctg ccggccggag tgaagcgcag cttctccggg
    10501 gtgaacaaca ggacggtccc gggtatggga ggctgggtca ccgccgtctc gtagaagttc
    10561 ttcagctgct tcactttcgc gcgcgagagc gtgcgctgga acgccttgtc ggtgcgctcg
    10621 atcttggaga tgaactgggc gatctcgtcg tcgtgggaga tgcgcgccgg ggcgatctct
    10681 tcgccctcgc cgtagaaacg gctgatgaag cgcacccttt gcagcagctc gggcgcgcga
    10741 aagctgccga aatagaagat gccgtctttt tgccgaatct gggtggcgag cacaggtctt
    10801 ctccccgtct tggtgggctt gagccgcggg caccgaacgc cgaactatag cccagccggg
    10861 gccgtgctga caaaatccgg acttcgtacc ctttgcggcc tccaaattct ctcgccgccc
    10921 ctctggtaaa gtagaccgct tcaccgcccg gcgtccgccg cggcgccgtc ctcgtttttc
    10981 ggagacccga tgcagaggag caccaagatc gtcgcgacgc tcggcccggc gtcgagcgac
    11041 gccgccaccc tggagcgcat gtttttggct ggtgtggacg tcgtccgcct caacttttct
    11101 cacggcaccg ccgaagacca cgtccggcgc gccgagatgg tgcgcgaggc gtcgcgcaag
    11161 accgggcgga ccgtgggcat catggccgac ctgcagggcc ccaagatccg catcggcaag
    11221 ttcaaggacg ggccgatcac gctcaagccc ggcgacagct tcatcctgga cgccgattgc
    11281 gagctgggcg acgatcgccg ggtcgggctc gactacaagg agctgccccg cgatgtgcac
    11341 ccgggcgaca tgctgctgct cgacgacggg cgcatcgtgt tcgaggtgga caaggtgctc
    11401 ggcaacgaga tccacgcgcg cgtcgagcac ggcggcgtgc tctcgaacaa caagggcatc
    11461 aaccgcctcg gcggcggcct caccgcgccg gcgctcgccg ccaaggacat ggaggacatc
    11521 cgcaccgccg cgaagatccg cgtcgattac ctggccgtct cgttccccaa atccagcgcg
    11581 gacatgtaca tggcgcgcca gctcatgcgt gcggccggcg gcgacgcgtt cctgatcgcc
    11641 aagatcgagc gcgccgaggc ggtcgagccg cgcgcgctcg aggacatcat gtcggcctgc
    11701 gagggcatca tggtcgcgcg cggcgacctc gccgtggaag tgggtgatgc ctcggtcccc
    11761 gcgctgcaga agcgcatgat ccgcatggcg cgcgagcaga acaagatcac catcaccgcg
    11821 acgcagatga tggagtcgat gatcaacagc ccggtgccga ctcgcgccga agtgtccgac
    11881 gtcgcgaacg ccgtgctcga cggcaccgac gcggtcatga cctccgcgga aaccgcgagc
    11941 ggcaagtacc cggtcgagac gatcaccaac atggcgcgca tctgcagcgt cgccgagcag
    12001 tcgcagccgg tgacgctcga tcgcgacttc ctcgaccgca cgttcacgcg cgtcgaccag
    12061 tcgatcgcga tggcggcgct gttcacggcc taccatctca aggtcaaggc gatcgcggcg
    12121 ctcaccgagt cgggatcgac cgcgctgtgg atgtcgcgcc tcaactgcgg cgtgcccatc
    12181 ttcgcgctga cctcgcagac gagcacgcgc taccgctgcg cgctgttccg tgacgtctat
    12241 ccgctcatgg tcaagtacgt cggacacgac cgcgaagagc tgctcgccga ggcggagaac
    12301 gtactcgtga agaacgacgt cgtgcaggcg ggcgacctca tcgtcctcac catcggcgag
    12361 ccgatcggca aggcgggcgg caccaacacc atgaagatcg tccgcgtcgg agagcaccgc
    12421 cgcgggtagg gccggccccc gagttccttc caggaagaga acgaacatgg gagagaagag
    12481 gctcggcatc atcatgcacg gcgtgaccgg gcgcatgggc acgaaccagc acctggagcg
    12541 ctcgatcgtc gcgatccgca agcagggcgg cgtgcgcctg tccgacggca cgcgcgtcat
    12601 gcccgatccg gtgctggtcg gccgcaacgc cgccaagctc caggcgctcg catcccggca
    12661 cgggatcgag cgctggacca ccgatatcga ggtcgcgctg gcgaacgagg gcgacgccgt
    12721 cttcttcgat gccgcatcga cgcagctgcg cccggcgctg ctgcggcgcg cgatcgcccg
    12781 ggggaagcac gtctactgcg aaaagccgat cgcgaccacg ctcgcgcagg cgctcggcgt
    12841 cgcgcgcgcc gcgcgcaagg cacgcgtcag gcacggcgtc gtccaggaca agctctggct
    12901 gccgggcctt ctgaagctgc agaagctgcg cgactcgggc ttcttcggcc gcatgctctc
    12961 ggtgcgggga gaattcggct attgggtatt cgagggcgac cggcgcgagc aaccagcgca
    13021 gcggccgtcg tggaactacc gcaagccgga cgggggcggg atcatcctcg acatgctgtg
    13081 ccactggcgc tacgtcctcg acaatctgtt cggcgaggtg cggcgcgtgt cctgcctcgg
    13141 cgcgacgcac atcccggcgc gctgggacga gacgggccgc aagtaccggg tgaccgcgga
    13201 cgacgccgca tacgccacgt tcgagctgcg aggcggcgtg atcgcgcacg tcaacagctc
    13261 ctgggcggcg cgcgtgcggc gcgacgatct ggcggtgttc caggtcgacg gcacgcacgg
    13321 ctcggccgtg gccggcctga cgcgctgctt cgcgcagtcc ctgaacgcca cgccgcggcc
    13381 ggtgtggaac ccggacgtgc gccaggccca cgatttcctc gccgactggc aggaggtccc
    13441 ggacgacggc gtctacgaga acgcgttcaa ggccgaatgg gagctcttca tccgacacgt
    13501 atgcgagggt gctccgttcc gctggaatct cgtcgaggga gcgaagggcc tgcagctcgt
    13561 cgactgcgca ctgcggagct ggaagtaccg gcgctggatc gacgtgccga agctgaagat
    13621 ctgacccgcg ccgtgaacgc gcccgagaag ccgaagctga agatcaggtt gcccgccggc
    13681 ggccgccgtc tcgaagagta ccgcctgcgc gggccgcgcg acttcgcgat gctcgggaag
    13741 ccgaagttcg accgcatcgc gctcgcggcg gcgcacgtcg tcggcgatcc gtgctcggcg
    13801 cgcgacccct ggctcgaggc cgcagtcgac tgggacgcga cgctcgccta ccggcgctac
    13861 ctctggtcgc tgggtctggg cgtcgccgag gccatggaca ccgcgcagcg cggcatgggc
    13921 atggactgga gcgcctcgct cgagctcgtc cgccgctcga tcgacgcgtc gcgcgacgtc
    13981 gaaggcgctg tgctcttcag cggcgccggc accgaccagc tcacccccgc ggccgccgta
    14041 aagcccgccg acgtgctggc ggcgtacgag gagcagtgcg gcgcgatcga ggcgctcggc
    14101 gggcgcatcg tgctcatggc gagccgcgcg ctcgcggcct gcgcgcgcgg cgcggacgac
    14161 tacctcgagg tctatgcgcg ggtgctgtcg caggtgcgcg agccggtgat catccactgg
    14221 ctgggcgaca tgttcgaccc ggcgctcgcc ggctactggg gcagccgcga cccggcggcc
    14281 gccatggaga cggcgctcgc cgttgtgagc gccaacgctg ccaaggtcga cggcatcaag
    14341 atctcgctgc tcgacaagga caaggaaatc gcgatgcgcc gccggctgcc ggcgggcgtg
    14401 cgcatgtaca ccggcgacga cttcaactac cccgagctga tcgagggcga cgccgagggc
    14461 cattccgacg cgctgctcgg catcctcgac gcgatcgcgc ccgccgcgtc ggccgcgctc
    14521 gcctggctcg ccgccggaga ccgcgagggt ttccgcagcc tgctcgcccc gacgctgccg
    14581 ctgtcccggc acgtattctc ggcgccgacg cgcttctaca agacgggcat cgtgttcctc
    14641 gcctggttga acggccacca ggaccacttc gtcatggtcg gcgggcagca atcggcgcga
    14701 tcgatcgctc atctcgccga gacattccgg ctcgccgacg ccgcggggct gctgcgcgat
    14761 ccggagctcg cggtggcgcg catgaagcgg ctgctcgccg tgcacgggct cgacgcgcga
    14821 tgacccgtcc gggcccgccc gacgcggggc tgctctcgct caacaccgcg accgtgcgcg
    14881 agaagtggag cctgcgcgag atcatcgcgg gttgcgcgcg ccaccgcatt ccgggcatct
    14941 cgccctggcg cgaccagctc gcgcagctcg gcgccaagga agccgcgaag gtgatccgag
    15001 accacggcct gcgcgtcacc ggcctgtgcc gcggcgggat gttccccgcc gccgaccgca
    15061 agggccggcg cgcggcgatc gacgacaacc tgcgcgcgat cgacgacgcg gcggtcctcg
    15121 aggcggcgtg cctcgtgctg gtcgtcggcg ggctgccggc gggctccagg gacctcgcgg
    15181 gggcgcgcga gatggtgcgc gacggcatcg gcgagatcct cgaccatgcg cgctcggccg
    15241 gcgtgccgct cgcgatcgag cccatgcacc cgatgtactg cgcggaccgc ggctgcgtca
    15301 acacgctcgc gcaggccctc gatctgtgcg accagttcgg ctcgggttcg agccggggtg
    15361 atctcggcgt agtcgtggac gtctaccacg tctggtggga ccccgacctg aaggcacaga
    15421 tcgagcgcgc gggcgacagg cggctgctcg cgtaccacat ttgcgactgg ctggtgccga
    15481 ccaccgacct gctgctggat cgcggcatga tgggcgacgg cgtcatcgac ctgccgctgg
    15541 tgcgctcgtg gatgagcgag gccggatacc gggggttcca cgaggtcgag atcttctcgg
    15601 cgcgcaactg gtggaaacgc gatcccgacg aagtgctgat gcgttgccgg gaacggcacc
    15661 aaatcgccag ttgacggtcc aaggtccccg gaaaggcccg gtgcggcgtg gcagccgcac
    15721 cgccgcccgg gtaaaatcgc ggcctctccg cgtctattca gaacgagccc gaaactcagt
    15781 agaggaacgt tgcatggaca ccaatcttct ttccaagacc gccgcagcca tggtcgccaa
    15841 gggcaaaggg ctgctcgccg ccgacgaatc gagcggcacc tgcgagaagc gcttcaagtc
    15901 ggtcggtgtc gagagcaccg agtcgaaccg gcgcgcttac cgcgatctgc tgttcaccac
    15961 caagggcatc gaggattacg tgagcggcgt gatcctgttc gacgagacgc tgcgccaaag
    16021 cacggtttcg gggaacgacc cgttccccca atacctgcag aagaagggcg tcatccccgg
    16081 catcaaggtc gacaagggca cgcacaacct ggcgctcgcg ccgggcgagg tcgtgaccga
    16141 gggcctcgac gggctgcgcg gccggctcga ggagtacttc aagctcggcg cgcgcttcgc
    16201 caagtggcgc gcggtgatca acatcggtcc cggcatcccg acccacgcct gcctgtacgc
    16261 gaacgcgcac gcgctcgcgc gctactccgc gctgtgccag gagcaatcga tcgtcccgat
    16321 cgtcgagccc gaagtgctgc tcgatggcaa ccatacgatc gagcgctgct tcgaggccac
    16381 ggaggcgacg ctcgcggcgc tgttcgcggc gctgcgcgcg cacaacgtgt cgctcgagca
    16441 cacggtgctc aaggcgtcga tggtggtccc cggaaaggac ggcggccgcc aggcgagcgt
    16501 cgacgaggtc gccgagctga cggtcagatg cctgaagcgc accgtgccgg cggcgctacc
    16561 gggcgtggtg ttcctctcgg gggggcagag cgacgaggcc gcgacggcgc atctcaacgc
    16621 catggcgcag atgccgaacc tgccgtggcc gctcaccttc tcgtacagcc gcgcgctgca
    16681 gaaccccgcg ctcaagacct ggtgcggtaa ggcaggcaac gtcgccgcgg cgcagcgcgc
    16741 gttctatcat cgcgcgcgca tgaacagcct cgcggcgcag ggcaagtacc ggcccgagct
    16801 cgagaagcag gcggcctgat gagcgcctca gccccgctgt acgaatcggg tctgcagagc
    16861 ctgccgctgc tcgggcgcgg caaggtccgc gacatgtacg cggtcggaag cgaccgcctg
    16921 ctgatcgtca cgaccgaccg gctgtcggcc ttcgacgtcg tcctccagga tccgatcccc
    16981 gacaagggcc gcgtcctgaa cgccatgtcc aacttctggt tcgccaagct cgcgcacatc
    17041 attcccaacc acctgaacgg cgcgtcgccg gagtcggtcg tgaaaggtga gcgcgaggcg
    17101 gtgcaggttc gcgggcgctc cgtggtcgtg cgcaagctca agccgctgcc gatcgaggcg
    17161 gtggtgcgcg gctatctcat cgggtccggc tggaaggact accaggcgac cggcggcgtg
    17221 tgcgggattc cgctgccgaa gggcctgaag caggccgaca agctgccgga ggtcatcttt
    17281 acgccggcga ccaaggccga aatcggcgcg cacgatgaga acatctcctt cgagcaggtg
    17341 gagaagacca tcgggcgcga cctcgcgaag caggtgcgcg atgtgtcgat ccgcctctac
    17401 cgcgaggcgg ccgattacgc gcgcgagcgc ggcatcatca tcgccgatac caagttcgag
    17461 ttcgggctgg acgacgcggg gcgcctggtg ctgatcgatg aggccctcac cgccgattcc
    17521 tcgcgcttct ggccggccga tcagtaccgg ccgggctcga gcccgccgtc gtacgacaag
    17581 cagtacgtgc gcgattatct cgagacgctc gactggaaca agaagccgcc ggcgccccgt
    17641 ctgccgcccg acgtgctcgc caggacttcc gagaagtacc gcgaggccct gtaccgattg
    17701 accggacaga aactgggagg atgaaacgat ggccagcagc acgccgctcg tcggcatcgt
    17761 catgggcagc acgagcgact gggaagtaat gcaacaggcc gcgaagcagc tcgccgactt
    17821 ctcggtgccg tacgaggcgc gcgtgctttc cgcgcaccgg acgcccgagg cgctcgagca
    17881 atggctcgac gatctcaccg cgaaaggcgc gaagtgcttc atcgcgggcg cgggcggcgc
    17941 ggcgcacctc gccggcgtgg tggcggcgaa gaccacgctg ccggtgctcg gcgttcccat
    18001 gccatccaag cacctgcagg gactcgactc gctgctctcg atcgtgcaga tgccgaaggg
    18061 tatcccggtc gcgaccttcg ccatcggcga tgcgggcgcc gcgaacgccg gcctgttcgc
    18121 agtcgcgatg ctcgcgctcg ccgacgagaa gcttgcgaag cgcctgcagg atttccgccg
    18181 cgcgcaggcc gacgccgtga agaacgcgcg gctgccggcc tgagcgcgag gcgcgaagcc
    18241 ctttggcgtc ggagcccgta gcgcgcgccg tcgagatcct cgggcgcggc gggctggtcg
    18301 cgttcccgac cgagacggtg tacgggttgg gcggcgatgc ctccaatgac gccgccgtcg
    18361 cacgcattta ccgcgtgaag ggccggcccg cggatcaccc gctgatcgtg cacatcggcg
    18421 acgcgtcgct gctcagtcgc tgggcgcggg acgtgtcggc ggacgcgcgc aagctcgccg
    18481 agcgcttctg gccgggaccg ctcacgctcg tgctcaagcg cgcggcgacg gtgagcgacc
    18541 gcgtgacggg cggccaggac acggtcggcc tgcgcgtgcc cgggcatcct ctcgcgctcg
    18601 agctgctgcg cgccttcggc ggcgggatcg ccgcgccgtc ggccaaccgg ttcggcaagc
    18661 tgagcccgac gaccgcggat cacgtgcgcc gcgacctcgg atcggacgtc gacctcgtcc
    18721 tcgacggcgg cccctgcgag gtcggcatcg aatcgacaat cgtcgatttg tcgcgcggcg
    18781 ctccggtgct gctgcggccc ggacgcatcg gcgcggccga tatcgcggcg gcgatcggcc
    18841 acgatccacg gccgcgcgac gccgccgccc cgcgcgcgcc cggcgcgctt gaagtccact
    18901 acgcgccgcg tcatccgctg cgcctcgtga gccgcagcga gctcgagccc gcggtgacgc
    18961 gcggatcggc ggtgctcgcg ttcgaggagg cgcccgcgag cacggccgcg acgatgtgga
    19021 cgcgcgcctc gtccgacccg cagcgctacg cgcacgacct gtacgcgctc cttcgacggc
    19081 tggacgaggc ggccgcggcg acgattctcg tcgagcggcc gcccgaggga cccgggtggg
    19141 aggccgtgct cgatcgactc acgcgagcgg cgggtcgctg agcccgaatt ccaattgcaa
    19201 atcaaaccga agcgccccaa actagggccc ggcgacgttg tctggagggg gctttgaggt
    19261 tagaattcag cccgcgtggg gttgtagctc agctgggaga gcgtcgcgtt cgcaatgcga
    19321 aggtcgggag ttcgatcctc ctcaactcca ccagctcttt ccctcgactg cgccgcggaa
    19381 cctccgcggc atggaccatc ccgctcctgc ttcttgcacg ggcacggtgt tgtcgcccgg
    19441 gggcggctcg cgacacgagg cctgaatggc gggaaagctg agggtcctgc tggtcgagga
    19501 ttccgaggac gatgccgcgc tggtgctcgc cgagctcaag cgcgccggat acgagctgct
    19561 ctcggaacgg gtctacagcg cgcaggccct gcaggatgcg ctgtcgcggc gcgaatggga
    19621 cttcgtcatc gccgactaca gcatgccgtc gttcaccggc ctcgaggcgc tgaagatggt
    19681 gcgcgggagc ggtcgcgacg tgcccttcat cgtcgtttcc ggcactatcg gggaagacgt
    19741 cgccgtggac gcggtgaagg ccggcgccaa cgactacctg atcaaggacc gcctcgcgcg
    19801 cctcgcgccg gcggtcgagc gcgagcttcg ggaggccaca gagcgccgcg accgcagatc
    19861 cgcggagtcg gcgctgcgtg aatccgagga tcgctaccgc ggcgcgctgc aggaggcaaa
    19921 cctgagactg cagtcgctgt cgagccgcat gctggaggtg caggagagcg agcggcggca
    19981 catcgcgcgc gagctccacg acgagatcgg acaggcgctc acggcggtga agctgcatct
    20041 gcaatcggcc atgcgccgct gcgcgcccga ggcgaggccg ccgctcgagg aatgcgtgct
    20101 catcaccgat caggccctcg accaggtgcg caacctctcg ctgaacctga ggccttcgca
    20161 gctcgacgat ctcgggctga tcgcggcctt gcgctggcag atcgggagac aggcggccgc
    20221 gtcgggcctg cacaccgagt tcgaggccga cgagctcagc gagcgcctcg agcccgcgct
    20281 cgagaccgcg tgcttccgct tcgtgcagga ggcgatgacg aacgtcgcgc gccacgcgca
    20341 ggccagcaac gcctggatcg aggcgcggcg caagggcgac cggctgcgcg tgagcgtgcg
    20401 cgacgacggc cgcggcttcg acgtggcgga ggcgacgcgc gaggcgatcg cgcgcggcag
    20461 cctcggattg ctcggtatgc aggagcgcgt cgggctcgcg ggcggcagat tgaacgtcag
    20521 ctcgcgaccc ggcgaaggca cgaccatcgt cgccgaattc ccgcttcgtt tcgcgccccc
    20581 gcgcgcgcgc agggcccgga ggaacgacaa tgccggttcg agtactgctc gttgacgatc
    20641 acacgctggt gcgggcgggc attcgctcgc tcctcgacgg catggaggga ttcacggtcg
    20701 tcggcgaggc gagcgacggc cgcgaggcgc tcgcgcagct gaaggcgcag cagcccgatg
    20761 tcgtgctgat ggacatcgcc atgaaggacc tgaacggcct ggagaccacc atccagatca
    20821 agcgcgattt tcccggcacg cgcgtcgtca tcctctcgat gcacgggacc ggtgattacg
    20881 tgcagcaagc gatccgcgcc ggcgcctcgg gctacctcat gaaggacgcc gccaccgccg
    20941 agctcggcat cgcgctgcag gcggtgatgc gcggagaggt gtatctgagc ccggccgtct
    21001 ccaagcaggt ggtggaaagc tacatgctgc ggccgggcgg cgacaagcac ccggccgagc
    21061 agctgacgcc gcgtcagcgc gaggtgctgc agatgatcgc cgaggggctc tcgaccaagg
    21121 agatcgccta tcgcatgggc gtcagcgtca agaccgtgga gacccatcgc acgcagctca
    21181 tggagcgcct ggagatccgc gacgtcgccg gtctcgtgcg ctacgccatc cgcatcgggc
    21241 tcatcagcgc ggaaaagtaa cgcgccgggt ctgccgttcg gcacccctag ggtatttcga
    21301 acccggtttt cgggaggcgc ccgattggtc gtccatggcg tacgagatac cctgacgccg
    21361 tcaatggaac gcactgaaac tgcaggcaaa accagcgtcg cggaggcggt cgaatccgcg
    21421 cccgaccgga gaccgatgaa ggtacttctc gtcgacgaca gcccgtattt catacgcgcc
    21481 gcgagccgct ttctctcgga gctggaagcg gccgtcgccg tcaccaccgc gggcaacggg
    21541 accgacgccg tgaaggcggt cctgagtgac aggcccgacc tgatcatcat ggacatcaac
    21601 atgccgggca cgggcggact ggcggcggtg cggcacatca aggccatcga gcccgacgtg
    21661 cgcgtcgtgg tggtctcgct caacgagtcg gacgatttcc gcgccgccgc gcgcgccgcg
    21721 ggcgccgacg gctacatcgc caagcgccac ttcgcgaccg gtatcgtcgc cttcttcgcg
    21781 agcgagctcg acccgccgcg gcccgcgtag cccgctagac cgacagcttc atcggcagga
    21841 tcaccatctg catgccgcgc agatggaatc ggcgctgcag caccgtcgcc gcctggttgt
    21901 gcagcaggcg cacgaaccag ttctcgttgt cgaagaccag cttgctcgtg aagaagatgc
    21961 tgttcgggaa ctcggcgtgc acgcgctgcg cgagccgcgt gaactcgtcg atcggatcgg
    22021 tcccgaacga aagataggat ttcgccgccc agcccttgct gttgcagtag ttgacgaaat
    22081 agctgagtgc cgacttcgcc tccacttcca tcgcgtccat ctgctggcgt ccgccgaagc
    22141 tctgcgagtc gaccgcgcgc gcgttgacga agatgaagtt ccggaaatgg ttcgggaaca
    22201 tgcgctgcac ccagagcagg gcatgcatgc cgccgccgcg cgaggagccg accaggaaca
    22261 ccgcggtcgg ctggcccgga tcgggcttgg gcgccgtggc cacgctgccg aacgggagat
    22321 cggcgtactg cgcgtcgagc ttgcggatct gctgccgggt ccagttgtag tgcatgcgga
    22381 tggcgatgca gaccccgacg atcgcgccga tgatcagggc cgtcagccag ccaccctcga
    22441 ggaatttctg cgccatcagc atgagcagga taccggcgca gaccacgaat cccgcggcgg
    22501 acagcaacaa gcgccgtagc cagtggcgtt cgcgccggtg ccatccccag tacacgcaca
    22561 ggccgaagag cgaagtggcg aacgtgagga acacgctgat gctgtagagc acgaccagcg
    22621 tggacacgct gccgccggtc cagagcagga tgccgagcgc tgcgacaccc atcaccagga
    22681 tgccgttctc ggtcaccagg cgcgccgaca ggtaccggaa tttgtgcggg acccacgagt
    22741 cgaacgacat gttggcgagc accgcgggac cgccgaggaa gcccgtgttc gcggcgacga
    22801 agagcagccc ggcctcgagc gcgagcacga cgatgaggcc ggcctggttc atccaggggc
    22861 tcgaccagcc gaagctctgg atgatcgagc cgaaggccac cgcgttgagc gtctgccctt
    22921 cgaccggctt cgcgtcccac agcaggtaca gcaaaacgat gccgcccgcg gtgaacgcga
    22981 gcgatatcgc catgtacagc atcgtcacct tgccggtatg cacgcgcggc tcggcgagca
    23041 cgttcacgtt gttggatacc gcctcgaggc cggtgtaggt accgcccccc tgcgagtacg
    23101 cgagaagcag cagtgccgcg acgccggtcc atccgagctg cgacgcgagg tcgtgcgtct
    23161 ccgagagggt ctcgggcacc agtgtgggca gctcgggcgc gtgggtgagg acgccgtaga
    23221 cgatgaggaa ggcgtgggtc acgatgaagc ccacgaatac gggcagcagg accgtgatcg
    23281 cttcgcgcat gccgcgcagg ttcaggccgg tcagcaccag gatcagcgtc ccctcggtga
    23341 tcagcttgta cggctggaag ctgagcggca gcagcgaaaa cagcgcatcg acgccgctcg
    23401 cgatcgagat ggcgatggtc agcacgtagt cgaggatgag cgccgcgccc gagaccagcc
    23461 cggcgtaggg accgagcagc cgggtcgaga cgcgatagcc gccgccgccg gtcgggaaca
    23521 gctcgatcac ctggttgtac gcgagcgcga tgatgaacac cgtgaccgcg accgcgagcg
    23581 cgaggtacag gcctagatgc gtgtggctgc cgagcgcccg gaacgcctcc tcggggccgt
    23641 agcaggaaga cgagatcccg tccgcgccca gcccgaccca ggcaaagaac gcgacgagcg
    23701 cgatgttgtg ccgggtccgg gtatcgagcg gatcgagcgg cttgccgaag agcttgcgcc
    23761 tgagggcggc gagcggcatg tcagcggtgt cgcccggcgt cgagctgcct ttcgatccgg
    23821 cgcgagaaca cggtcatctc cttcgcggcc tgcttgtcgc ctttgcgctc ggcgacggcg
    23881 atgccctcgc ggtaggcagc gagcgcgtcg gcgaggcgtc cggagtcggc gagcgccttg
    23941 ccgagcagtt tccacgccgc ggaatacccg gggtcgcggt tcaccgcttc gcgcagctgc
    24001 gcttcggcgt tgtcggcgtc gccggccttc aggtattcgt tgccgagcga atagcgcagg
    24061 agcgctccgt cgcgcggcgt cccgatcagc ttctgcagat tggcgatgac tggggatgtc
    24121 atttaagatg gggccggacc gcccgctccg gagcgattat aagtccgcgg ttcccgggtc
    24181 gctgtcggcg cggcccgcat tgataagctg cccattgtcg aagagcgccc gcaacgccgc
    24241 cgacgcccac gtccgtccga cttcgcgcga agcgtcctcc gccgcgaagc ggctcgagcg
    24301 gctcggcatc cgccgcgaat ttgatctcgt tctgcatctg ccgctgcgct acgaggacga
    24361 gacgcgcatc acgcccatcg ccggtgcgcc ggcgggcgcg gcggtccagg tcgagggcgt
    24421 ggtgcgctcc accgatatcg cctaccgtcc gaagcgccag ctcgtctgcc gcgtcgcgga
    24481 cgagtcgggc gaggtggtgt tcaggttctt caggttctgg gcgagccagg tgaaggcgct
    24541 ggcgcccggg gtcagggtgc gcgcgttcgg cgagctgcgc tcgggcttct tcggcggcga
    24601 gatgatccat ccgcgcttcc gcgtcctgcg cggcgagtcg ccgctgccgg cggcgctcac
    24661 tcctgtctat ccgacgacgg cggggctgtc gcagctcgag ctccggcgcg ccatcgagac
    24721 cgcgctcgcg cgcgcgcagc tcgacgacac gttgcccgaa gcgctgcttg cgcgcgtcgg
    24781 actgtgcggc ttccgcgaag ccatcgacgc gctccatcac ccgctcccgg gcgcggacag
    24841 ccacgacctg ctcgcgcgcg ccgagccagc gtggcggcgc gtcaagttcg acgagctgct
    24901 cgcgcagcag ttgtcgatgc gcaagcacta ccgagagcgt aaggcgcggg ccgcccgggc
    24961 gctcgccccg aaacgcacgc acaccgcggc gctgatcgag cggctgccgt ttcgcctgac
    25021 cggcgctcaa ggccgcgcct ggagcgagat cgagcgcgac ctcgcccagc cgcatccgat
    25081 gcaccggctg ctccagggcg atgtcggcag cggcaagacc gtcgtcgccg tgctcgccgc
    25141 gctgcgcgcc gtcgagcacg gcctgcaggc ggcggtgatg gcgcccaccg agatcctcgc
    25201 cgagcagcat taccgcaagt tctccgaatg gctcgtgccg ctgggcttgc cggtcgcgtg
    25261 gctgagcggc gcgcagtcgg cgaaggagag aaaagcggct ctcgcagcgg ccgcgtccgg
    25321 cgaagcgccg atcgtcgtcg gaacgcacgc gctcatccag gagaaggcgc ggctgcgcgc
    25381 gctcgggctc gcgatcgtcg acgagcaaca ccgcttcggc gtcgagcagc gcctcgcgct
    25441 gcgggcgaag ggcgccgagc ctcacgtcct catgatgagc gccacgccga ttccgcggac
    25501 gctcgcgatg agcttctatg ccgatctcga cgtttcggtg atcgacgagc tgcccccagg
    25561 ccgccggccc gtgatcacga agctgatcgc ggactcgcgc cgcgccgagg tcgtgcagcg
    25621 catccgcgcg gcctgccgtt cggggcgcca ggcgtattgg atatgtccgc tcatcgagga
    25681 atccgagcag ctgcagctgc agaccgtcct cgaaacgcac gcggtcctcg gcgccgaact
    25741 ctccgatctg cgcgtcgggc tgctccacgg aaagctcaag cccgcggaaa aggccgagac
    25801 gatggcagcg ttcgtgtcgg gcgagatcca gctcctcgtc gcgaccaccg tcgtcgaggt
    25861 gggcgtcgac gtcccgaacg cctcgctcat ggtcatcgag cacgccgagc gcttcgggct
    25921 cgcgcagctc caccagttgc ggggccgcgt cggccgtggc gcggagcagt cggtgtgcgt
    25981 gctgctgttc cagaaccctc ccggaaacgc cgcgcgccag cggctgcgcg tcatctacga
    26041 gaataccgac ggcttcgcga tcgcgcgcga ggacctcgcg ctgcgcgggc ctggcgaggt
    26101 gctggggtca cgccagagcg gcgctccact gctgcgcttt gccgacatcg tcgccgacgc
    26161 cgagctgctc gagctcgcga gcgccgccgc cgagcgcctg ctcgacgagc atcccgaggc
    26221 cgcgcaacgc cacctcgacc gctggctcgg cggccgggtc gagttcctgc gggcatgagc
    26281 gccgggcgcg ctagaattcc ccggccatga gcttgcgcgc tattaccgac cgcatctcgc
    26341 tgtacgagcg cctgatgcgc ctcgacaagc cgatcggcac gctgctgctg ctgtggccga
    26401 ccctgtgggc actgtggttc gcttcggcgg ggcgtccgga cgcgtggatc gtctggatct
    26461 tcatcctcgg cacgctcctg atgcgctcgg cgggctgcgt gctgaacgac atcgcggact
    26521 ggcaatacga cgccaaggtg aagcgcacgc gcgagcggcc gctcgcgacc ggcgcgctgg
    26581 gacgcggcga ggcgctcgcg ctggcggcag tgctttcgct cgtggctttc gggctggtcc
    26641 tgacgctcga tcgtctcgcg atcatcctgt cgttcgtggc gctgggcctc gccgcgacct
    26701 atccgctcac caagcgcttt ctggcggtgc cccaagcgta cctgggcgtc gcgttcggct
    26761 tcggcattcc catgggatac gcggcggtcc tgggaatcgt gccggcggtc ggatggatgc
    26821 tgctcctcgc gaacatcttc tgggcgatcg cctacgacac cgaatacgcg atggtcgatc
    26881 gcgacgacga cgtgcgcatc ggcatcaaga cggccgcgat caccttcggc cgcttcgacg
    26941 tggccgcggt aatgatctgc tacgcggcca cgctgctggt gctggccggc atcggggcgc
    27001 attaccgcat ggggccgctg tactacggcg gactcgccgt ggcgggggcg ctcatgctct
    27061 accactaccg tctcgtccgc acgcgcgatc gcgacgcgtg tttccgggcc ttcaaccaga
    27121 acaactgggt cggtgcggcg gtcttcgccg gcatcttcgc cgacttcgcg acgaaaggac
    27181 tctatccgtg gatctcggtc tgaaggggcg cgtcgtgctg gtcaccggtg gtagcaaggg
    27241 aatcggcctg gcctgcgccg gcgcgttcgc cgcggaaggc gcccgcgtcg cgatcgcttc
    27301 gcgcagtcgc gacaatctcg cggccgcaga ggcgaagctc agggcggcag gacacgacgc
    27361 gacgtcgatc gccgccgatc tcggcaaccc gcgcggcgcg atggcggccg tggccgagac
    27421 cgagcgccgc ttcggcccga tcgacgtcct ggtgaactcg gcgggcgcgg cgcggcggca
    27481 ttcgcccgag gagctcgaca tgcaggcctg gcacgaggcg atggacgcga agtacttcac
    27541 ctacgtgcac gcgatgcagg cggttctgcc cggaatggcg gcgcgcgggc gcggcgcgat
    27601 cgtcaacatc gtcgggcagg gaggcaagat cgcgagcccc gcgcacctcg ccgggggcgc
    27661 ggcgaacgcc gcgctcatgc tgctgtccgc ggggctggcg gccgcctacg gcaagaaggg
    27721 cgtgcgcgtg aacgccatca atccgggtgt cacgctgacc gagcgcgtga ccggacgctt
    27781 cgaggcggag ggcaagaccg ccggcatctc gcccgatgag gcccggcgac gcggcgagca
    27841 atcgatcccg ctcggtcgct acgggactcc ggacgagatc gccgcggtgg cggtgttcct
    27901 ggcctcggac cgcgcgagct acgtgaccgg cgccaatatc ccgatggatg gcggcgccgc
    27961 ggccacggtg gtctagtggc cgaccgcgac ctgcgcgcgt ttctcgcgcg gctcgaggcg
    28021 ctcggcgagg cgaagcgcgt gcgcgccgag gtggacccgc gcctggagat gaccgagatc
    28081 tgcgaccgcg tcctgcgcgc gggcggcccg gcgctcgtct tcgagcgccc gtcggggcac
    28141 tcgattcccg tcgtcgcgaa cctgttcggg accgtgcggc gcgtggcgct cgccatgggc
    28201 gccgacgcct cggccgatcc ggtggcctcg ctgcgggaga tcggaacgct gctcgcgttc
    28261 ctgaaggagc ccgagccgcc caaaggcgtt cgcgatgcgc tcgagcgctg gctgccgatc
    28321 ggtcggcacg tgatgcacat ggccccgaag gaggtgcgtt cgccgccgtg ccaggagatc
    28381 gtccgcgaag gcgccgacgt cgacctgacg cgcctgccgc tgcaaacgtg ctggcccggc
    28441 gacgccggtc cgctcgtcac ctggggcctg acggtgacgc gtggcccgca caagccgcgg
    28501 cagaacatcg gcgtctaccg ccagcagctc gtcgcgcgcg acaagctgat catgcgctgg
    28561 ctcgcgcacc gcggcggcgc gctcgatttc gccgaccact gcgcggcgca tcccggcgag
    28621 ccgtttccgg tggccgtcgc gctgggcgcg gacccggcga ccatccttgc cgccgtcact
    28681 cccgtgccgg acacgctctc ggaatacgag ttcgccggac tgctgcgcgg cgcgaagacc
    28741 gagactgcga aatgtctcgc cggcgatctc cacgtgccgg cggcctcgga gatcgtgctc
    28801 gagggcgaga tccgtcccgg cgagacggcc gaggaagggc ccttcggcga ccacaccggc
    28861 tactacaacg agcgcgagcg gttcccggtc ttcaccgtgc ggcgcatcac cacgcgcaga
    28921 gacccgctct accattcgac gtacaccggt cgaccgcccg acgagccggc cgtgctgggc
    28981 gccgcgctga acgagatctt cgttccgatc ctgcagaagc agtttcccga gatcgtcgac
    29041 ttctatctgc ctcccgaggc ctgctcgtac cggctcgcgg tcgtgagcat caggaaacag
    29101 taccccgggc acgcgaagcg cgtcatgttc ggggtctgga gcgctctgcg gcagttcatg
    29161 tacacgaagt tcatcgtggt cgtcgacgac gacgtcgacg tccgcgactg gaagcaggtg
    29221 atctgggcgg tcgccacgcg cgtagacgcc gcgcgcgacg ccacgatcat cgagaacacg
    29281 ccgatcgatt acctggattt cgcgagcccc gccgccgggc tgggctcgaa gctgggtatc
    29341 gacgcgacca acaagggtcc cgcggagacg gcgcgggcct ggggcagacc gatcgccatg
    29401 gacgagtcgg tcaagcgccg cgtcgacgcg atgtggaaag acctgggact ctgaggcgaa
    29461 ggcgccgccg cgtcgccctg gcgcgtggcg cgcgggccgc tcagaagcgg tacatcaggc
    29521 ttgcggtcgc catccgcatc cgggcccggc ccgtgttgtc ggaatcgccg accgtgccat
    29581 agtcctcggc ctcgatgcgc aggccgagcc ggccggggaa gtcgatctgc atgccgaccc
    29641 cgaggatcgt gctgatcttg ctcttggtgc cgctcacggg cacgccgctg cccgccaccg
    29701 tatcggtgat cgtgccggtc gcgttgatcg attccgtcgt cgtgaccgac ttcatctggc
    29761 cgaggcgacc gaagagcgag aagctgtttc cgatgggaag gaccgcgagg acgctgcccc
    29821 accacccggc agacttatag gtctcgacca ctgagccgag gccgcttccg gtgccgtcgt
    29881 aggtgtgcga gaacttgccg aagttcgtcc gtcccagctc gagcgcccag taacggctga
    29941 gcttggcgcc gaaggtcagc ttgtagccct tgtcgctgct gtccttgctc tcctggatgc
    30001 ccggcacgcc ggtcgcgaag tcttcctgcg tgaatcgcgc gcgcgtcgca ccggcgccga
    30061 ggccgacgta ggcgccctcg agcacgttcc tgtcctgcgc gtaagagaat gagctggaac
    30121 cggcgagcaa cgcggccacg atacacggcc atgtgaggtt tttcatcggg ctccctgatc
    30181 gttgcgttga aaacgtgtct gcctcgaccc gaccgatgat atcccatagc gaaactcggg
    30241 agcaacaaag gagcgccgcg ggagcgaatt attcgcgcat ttgcgatcgg gcctcggatc
    30301 gatccacgtc tcgccttccg cctccggctt cgcgcgggac agcgcggccg gcttcgtaga
    30361 cccagcgcag caactggtcc tgcgccgcct ccgcggcggc ggcgttcgga tggttgacga
    30421 ggaagaccac ggcgaagcgg cgcccgtcgc gcgcaagcac gtaaccggcg agcgtgcgca
    30481 cgccggcgag cgtaccggtc ttgatgtgcg cctgtccggc gacgtcgctg ccgtgcaggc
    30541 gcctgcgcat cgtgccgtcg gatgcgacga cgggcatcga cgcgaccagc tcggacatga
    30601 ccgggctgtg ccaggccgcg acgagcagct tgccgaatgt caaggcgctg atgcggtcgc
    30661 tgcgggacag gccggcgccg ttctccagcg cgatctcgtc ggcggcgatg cctttgcgca
    30721 gcgcccaatc ccggatcagg ccctccgaga gatgctcgtc gccaggcaca tgcagcgcct
    30781 cggctgacaa ggtgaggaac agctggcgcg ccatcacgtt gtttgagaac ttgttgatgt
    30841 cgcgcacgac ttccgacagc ggcggggact cggcgctgta aatcagccgc gagtccggcg
    30901 caaccgtgcc atcgcgcacg ccgcccttga acgagccgcc cagctcgtcc cacagctccc
    30961 ggaacagacc ccacaggtag gagtcgtgcg agagcagggc gacgttccag gtgttctcgc
    31021 cgcacgcggc gggataggtg ccgctgaaat tcacccgggc gctcgcgtcg ttcgagtcga
    31081 attccgccct gatctgcgag cgccagtcgc cgcagggcgc gtcggatagc gccaccgagg
    31141 cgcgcagctc gatctgcgcc gggcgcggct cggggatcac gcgcagcgac ttcgcttcca
    31201 ggtccgggcc gaaatggaag cggatcgcat tgaaattcac cagcagcgcg tccgggccga
    31261 cgttgtacgc gcgcgccggc tcgttgtcga atttcccggg atcgtacggc gcgggggcga
    31321 agtagctgtg gtcgaggacg agatcgccgc ggatctcgcg cagcccgcgc tcgcgcagcg
    31381 cgcgcagcag gagccagaaa ttctcgatcg tgagcttcgg atccccggtg ccgcgcagca
    31441 cgaggtcgcc gtcgagaacg tcgccccgca tcggcccgcc ggcgtacgct tgggtcttcc
    31501 aggtgaagga aggaccgagc atctccaggg ccgcgtacgt cgtcaccagc ttcgccgacg
    31561 aggcggggtt catcgggcgc gaggcgttgt acgacagcat cggcgtgcgc gcggcgacgt
    31621 cctgcacgta gaccgccgtg gcgccgatcg ggatgccggc cgcggccagc gccttgcgca
    31681 gcgtcgcggg caggcgcgca ggctcggccg cgcccgccag gcacggcgcg agcgccagga
    31741 acgccaggat gaacgaccgc gcaagcgcgc ggcccgtgcc ggaccgcctc atgtggaggc
    31801 aagtatgcca gcgcacccgc gccccgggcg aggttcgctc agcgcagcag ctggcactcg
    31861 agctcgcgca ggcgaagctc cagggcctcg atgcgctcca ggtacgcgag cgcaagcgcc
    31921 atacccggcg cgttcaggcc gaagtcgtcg cgcaggcggg ccgcccggcg tccgaggatg
    31981 atcgcccgcg acgagaagcg ccagctcgcc tggtccgcgg cacgcgcccc gggtcggaga
    32041 tcgaagacgc cgagctcgat caggtcgatc aactcggcgc gcgccagccc ggacgcggcg
    32101 agcaactcgt ccatcgcgat ctcggtcgcc tcgtcgagaa actccaggat cgcatcgtcg
    32161 ctcatgcggg ctcgcctttt ttgagatgcc ggcgcgggtc gaagtgcgag gccgcagcga
    32221 gctcacggta cagggacttt tcgcgctcgc cgggttccgg gggcgtcgcg atcgagagga
    32281 cgcagtagag gtcgccgctc gttccccggg gccggggaag tccccggcca gggacgcgca
    32341 gcttctggcc tgctttcgcg ccgggcttca ccgagagctc gatgcggccg tcgagggttg
    32401 ggatctccac ctgggttccg agcaccgcct cccacggggc gagcggcagc tcgaggtaca
    32461 ggtcgtggcc ggacacccgg aacagcgggt gcggctcgag atggatgtcg aggtagagat
    32521 cgcccgcccg cccgccgttc acgccgggcc ctcccttgcc ggcgatgcgc aacctctcgc
    32581 cctcggtcgc ccctttgggc acccgcacac gcacggtctt cggcatgcgc ctgcgcacgc
    32641 cgtcggggcc catctcgggc acggccagct gcagagccag ctcggttccg cgcgccgcgt
    32701 cctcgagcga cagtcgcgcc tcgacctcgt agtcctcgcc ggcgatggcg aagtctgcgc
    32761 ccgcccggcc cgaaaagccc ctgccgggac gggcgccgaa gtgcgcgaac aagtcggcca
    32821 gatcaatgtc ctcgaacccg cccgggctga agcgggtctc ccaatcgggg ggcggacgaa
    32881 agtcctggcc gggctggtgg ctccccagct ggtcgtaggc cgcccgcttc tccgggtcct
    32941 tcagcgtctc gtaggcttcg gcgacttcct tgaagcgctc ctcggcatcg ggctccttgg
    33001 agacgtccgg atggtacttg cgggcgagcc ggcggtaggc cttcttgatg tcgtcctggg
    33061 aggctctgcg gtcgacgccg agcagggcgt agtagtcctt gtacttcatg gcggtcctgc
    33121 ttagatgtcg gcgggaccgc gcaattcaag tcgtaggctc ttgaaatccc gctcgccgaa
    33181 ccgtattatt agcactcgct acgaatgagt gctaagcacc gtagcgcctg atttcccggc
    33241 ccaatcgacg gtcgacctgt ctgtttgtgg ccactaactt cacaaaggag gcagttttat
    33301 gtccatgaac aagattcgtc ctctgcatga ccgcgtggtc gtcaagcggc tcgagcagga
    33361 gcagaagtcc gcgggcggca tcgtgatccc cgataacgcg gccgagaagc ccgaccaggg
    33421 cgaagtcgtc gccgtcggca acggcaagat cctcgaggac ggcaaagtgc ggccgctcga
    33481 cgtcaaggtc ggggatcgca tcctcttcgg gaagtactcc ggccagtcgg tgaaggtcga
    33541 aggcaccgag tacctcgtga tgcgcgaaga cgacatcatg ggcgtcgtcg ccagcaagta
    33601 agtccaacaa tccatcgaat caggagcaag ccatgcctgc caaagaagtc cgtttccacg
    33661 aggacgcccg ccataagatc ctggccggag tgaacgtcct cgccaacgcc gtcaaggcga
    33721 ccctggggcc gaagggccgc aacgtcgtgc tcgagcgctc attcggcgcc cccaccgtca
    33781 ccaaggacgg cgtctcggtc gcgaaagaga tcgagctcaa ggacaagttc gagaacatgg
    33841 gcgcgcagat ggtcaaggaa gtcgcgtcca agacctccga cgtcgccggc gacggcacga
    33901 cgaccgcgac cgtgctcgcc caggcgatct tccgcgaggg catcaagtac gtcgccgccg
    33961 ggatgaaccc gatggacctg aagcgcggga tcgacaaggg cgtcgccgcg atgaccgaag
    34021 agctgaagaa gatctccaag ccgtgcacga cctcgaagga gatcgcgcag gtcggatcga
    34081 tctcggccaa cgccgactcc gacatcggca agatcatcgc cgacgcgatg gacaaggtgg
    34141 gcaaggaagg cgtgatcacg gtcgaggacg gcaagggcct gaacaacgag ctcgaggtcg
    34201 tcgaaggcat gcagttcgac cgcggctacc tgtccccgta cttcatcaac agcgccgaga
    34261 agcagggctg cgttctcgag aatccctaca tcctgctgca cgacaagaag atctcaagca
    34321 tccgtgacct gctgccgatc ctcgagcagg tggcgaaggc cggccggccg ctgctgatca
    34381 tcgccgagga agtcgaaggc gaggcgctcg cgaccctcgt cgtgaacacg ctccgcggca
    34441 tcctgaagac ctgcgcggtg aaggcgccgg gcttcggcga ccgccgcaag gccatgctgg
    34501 aggacatcgc gatcctcacg ggcggccagg tcatcgccga ggaagtcggc ctgacgctgg
    34561 agaagacgac cctcaaggac ctcggccagg cgaagcgcgt cgaagtggcg aaggaagaga
    34621 ccaccattat cgatggcgcc ggcgacggca aggcgatcga ggcgcgcgtc aagaacatcc
    34681 gcactcagat cgaggaagcc accagcgact acgacaagga gaagctgcag gagcgcgtgg
    34741 cgaagctcgc cggcggcgtg gcggtgatca aggtcggcgc cgcgaccgaa gtcgagatga
    34801 aggagaagaa
//
LOCUS       DATDMW010000212        21678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1309026, whole genome shotgun sequence.
ACCESSION   DATDMW010000212 DATDMW010000000
VERSION     DATDMW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309026"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..818)
                     /locus_tag="VI319_09230"
     CDS             complement(60..818)
                     /locus_tag="VI319_09230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEY6864070.1"
                     /translation="MSTNKELDGKVALVTGGARNIGRAISRALAHAGAAVMVNANTSR
                     ADAEQTVSMIEGEGGRAAVHIADVTDGKAVKAMIDATVKKFGRIDVLVNNAAVRAETP
                     FADMTLEEWRRVIATVLDGAFLCTQACLPHIARAGGGAVVNIGGMTAHKGAEGRAHVI
                     AAKSGLAGMTRALALDLAPQGITVNCVVPGLIETVRGLPGAPERPEHRRGLPPVGRRG
                     EPDEIAAVVRLLCGPDSRYITGQAIHVNGGAYMP"
     gene            954..1739
                     /locus_tag="VI319_09235"
     CDS             954..1739
                     /locus_tag="VI319_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEY6864071.1"
                     /translation="MIVAGERVRVFWQPGCTSCLRTKEFLTKNGVDYESINVHGNPAG
                     MEALRKLGARSVPVVARGDKFVFAQTLTDVIKFLGLKIRLQERLSPDDLIAKLNVVLP
                     AAARYVRQIPADWLEKSFRNRKRPIRVLAHHVFRIPDGFLESMRDGRELTYERIMEEP
                     GPAIRTADDIARYGESVLGRFNDWWKAFPDKSCEKPMRTYFGEHPLHVVLERTVWHPA
                     QHTRQLMLILDTIGIEPDRRLTPGDLAGLPLPDKAWDDEKEAS"
     gene            complement(1767..2054)
                     /locus_tag="VI319_09240"
     CDS             complement(1767..2054)
                     /locus_tag="VI319_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864072.1"
                     /translation="MARSKPGFWSRGIDKLRGALLHGEPETASQAPKKKAEQKKPEKK
                     KPAQPARPATAVAKSPEPATTEAVASTATMDDKSPKRKIPVQPWYRHRQRW"
     gene            2114..3106
                     /locus_tag="VI319_09245"
     CDS             2114..3106
                     /locus_tag="VI319_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY6864073.1"
                     /translation="MKLQLSIGMASNPRTWPIFDGRVQPDGIELVASVVHPSELFWRQ
                     LKFADFDVSEMSFSTLMMARAQGDDRWVGLPVFTTRKLFHTEILVRRAAGIERPEDLR
                     GKRVAVPEYQQTAALWTRGALQHEFGVEPKDMEFWMERVPTHSHRGAIGFTAPPGVTI
                     RQIPPEKSIGGMMLAGEVDAVIHYIVNANLVDRSTVDLWSHPEIKPLFPDPVAEGVRY
                     YRKTGLLPINHGMVIRRELVERHPWVVLNLLKAFERASEIAERERLEHAAYHVATGLV
                     PPEGLQSLRTTVVRHGIKANRKVLETAAQYSLEQGLTSRRVGVEEVFAPSTLEQ"
     gene            3183..4169
                     /locus_tag="VI319_09250"
     CDS             3183..4169
                     /locus_tag="VI319_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864074.1"
                     /translation="MTSFRNFPTGAALLAALVAFAPVAVRAQADAYPSKPIRFILPFP
                     PGGGTDILGRLLAERLARRLAQPVVIENRGGAGGNVGAEAAARSAPDGYTIVLVAPSL
                     AISPSLYKKLAYDPVKDFSPVSLVATVPNVMVTNPSIPAKTLSEFIALAKSKPGAMNF
                     GSGGNGTSNHLAGELFNIVAGVRLVHVPYKGVNLAMNDVMSGDVQLVIIGIPAAAPHI
                     KAGKLRALAVIDSKRAAALPDVPTAAEAGLKDFDVTTWYGVLAPAGTPRPIVTRLNAE
                     IVAVMNSSEMKERLATMATEPVTSTPEEFGDLIRSEIVKWAKVVRAAGLKAD"
     gene            4172..4825
                     /locus_tag="VI319_09255"
     CDS             4172..4825
                     /locus_tag="VI319_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY6864075.1"
                     /translation="MYLPAHFEERSVETLHALIREHPLGALITLGPDGLTANHVPFEI
                     DPDPAPFGTLRAHVARANPVWREHPRDADALVVFQAESGYVSPSWYPTKRDTGKVVPT
                     YNYVVVHARGTVRAIEDPGWLRGLVSRLTDRHEAESGHPWKVTDAPEDFIESQLRAIV
                     GIEIRPHSLIGKWKVSQNRPAVDREGVVRALEDAGDAGSLDLARRVQDALRAKPGRV"
     gene            complement(4822..5187)
                     /locus_tag="VI319_09260"
     CDS             complement(4822..5187)
                     /locus_tag="VI319_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864076.1"
                     /translation="MSATDSVLNSWWLLPVLWVGLSMLASAALSTVRRDPAPRPEPEP
                     KIEEPVAPPPFLFDSPEASSEAIGWYMGVPIHRSARFDGIDYEFDRICPPQALRRAVG
                     ERVVAPGIVYTRRSTTRPY"
     gene            complement(6062..6649)
                     /locus_tag="VI319_09265"
     CDS             complement(6062..6649)
                     /locus_tag="VI319_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6864077.1"
                     /translation="MSAALDRRAVVAALLERREALLVVAGLGAPAWDCAAAGDHPLTF
                     PLWGAMGSACMVGMGLAIAQPGRRVLVVTGDGEMLMGLGSLATIGAQRPRNLSLVVLD
                     NERYGETGMQKTHTAHGVDLAAVARACGFSAARGVSAPGEIASMRTAVLEARGPLFFQ
                     VKVAAGASAPALPPRDGGYLKQRFREALLGSDALR"
     gene            complement(6646..7194)
                     /locus_tag="VI319_09270"
     CDS             complement(6646..7194)
                     /locus_tag="VI319_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY6864078.1"
                     /translation="MSNRNRRSSTEAAWPAAVHDVLKSRGVGQVAYVPDAGHRLLIEL
                     CHADRSIVTVPLTSEEQGIGLAAGAWLGGQRAALLMQSSGVGNCVNALTLGSNCGFPL
                     LLIVTMRGEWGEFNPWQIPMGQATEPILHASGVRTFRLERPEDAAATVDAAAALAFDG
                     YAMAAVLVSQKMLGAKSFAGAR"
     gene            7268..7720
                     /locus_tag="VI319_09275"
     CDS             7268..7720
                     /locus_tag="VI319_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY6864079.1"
                     /translation="MRILICLLTAAAALVCAQARAQNIYKWVDRNGRIHYSDVVPGKD
                     SKAIAIQDRLSIYSPEPAVAQALQLTPRSSQTVALADRVATLERQLQAERAARQPAPP
                     DTSAAYERCLADRRTDCDQLLSGAAAPGVDAPTAFRPGPATLRRASAS"
     gene            complement(7717..12846)
                     /locus_tag="VI319_09280"
     CDS             complement(7717..12846)
                     /locus_tag="VI319_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6864080.1"
                     /translation="MIAVWTAVAYQLHQLRSEAIDTARRHGENITAVVGEHFLVFAGS
                     IDGTLRHLREQWLRNPRRFSATVNEHISLSGHILDRLAVTDAEGLVAYPARPGRSLAE
                     TDYFAAARAGGVEQLLVGNPERVPGDARIFIPFALAIRSAGEFRGIVSAAISAGDLVR
                     AYKAIDLGPGGFITLRHHDGTVVARSSDFESLIGGPRPEWQELDASGPATGSLIRRSP
                     RDGIERIYSYRRLSGYPLVVVAGQELQGTLARYYEQRRAYLAGGTLLSLVLLLLVTVT
                     LGRLNRLALAEKDLRESENRYRALTGLSADWYWELDEKFRMLALTPNKPSPRAGEQAR
                     KHLGKRPWEMPYVDPADADWRRYRELLESRSLFRDIVLKATGRGGRVVYLNVRGHPVY
                     DGAGGFRGYRGITKDVTQEKRHERLLELEHRVNRCLATAEDVPSALEAVIRAICEAES
                     WECGRYWRVDGRSQMLRFQQGWGVEGDAMREFIERSRELAFAPGVGLAGIAWQTRTPL
                     WVPDVTKDARVTMVVARPFNMHGAFVFPVTAGGEVIGVLGFASHDVRQPDERLLQAIR
                     VVGSQIGQFVQRKEAEEVLRGSEARFRSLTKLSSDWYWEQDENLRFVAMNEDILGKTG
                     VSAASHIGKLRWDLPALNMSQADWAAHRAIVEAHQPFHDLELCRLAEDGRPSWMLISG
                     EPIFDGSGNFKGYRGIGKDITERKFEERLRALEHAVNRDLADADEVHACVKAVIRTVC
                     ESQGWDCGRYFAVDEEGQVLRFAEAWGVDDPAIQSFIESARKLAYRPGEGLSGCAWQS
                     GQPLWSTDVTKDPRSLTVQGKRSVASALGIHGSFVFPVVADGKTIGVLNFSSRQAREP
                     EERLLQAIHVIGRQIGQFVQRKQSEEDLQRFRAAMDASADMFWLIDPIGMRIVDVNDT
                     ACRTLGYTREELLALGPHDILSAPRAELAETYRRLIAGDGKEMVIAGSYRRKDGALVP
                     MESMRRVVPSGDGYVIVAAARDVTERLEREEELQRFRLAMDNSADMIVLIDRATMRFI
                     DVNRTACRLLGYSREELLQMGPHDVLPVGREELERSYDALIADPSRPSGMNTVYRCRD
                     GTHLPFESTRRVLRSGDKYIIAAVSRDIRERLAAEEALRRSNERFNLAVRATNDVIWD
                     WDLTTDEMWWNENLKRVFGYAPEASRTGEFWKRHIHPEDHDRIVKGIRELLESGGDNW
                     SDEYRFLRADGTYARVLDRGHAVRDSAGKAVRMIGAMTDITMRKEAEEKLAYLAQFDS
                     LTGLPNRHLFRDRLMQAMARARRSSKAMAVLFIDLDRFKLINDTLGHGAGDRLLKDAT
                     ERLQACVRSSDTVGRFGGDEFGAIITDLAKPGDASVVAQKIIDALARPFNLDGHESYV
                     TASVGITLFPTDGEEAGTLIMNADAAMYRAKEQGRNTYQYFTREMNERAMQRVTMESA
                     LRRAIERREFLLHYQPKVSITTGAVCGFEALLRWQHPESGLVPPLQFIPVLEDTGLIV
                     TVGDWVIAEVCEQIARWSREGFRVPPVAVNLSARQFQQKGLEETVAASLRASDIDPKL
                     LQFELTESLLMKEPEVAARTLRGLKELGVTLSVDDFGTGYSSLSYLKRFPIDALKIDR
                     AFIRDVTVDPDDAAITFAIIGLAHSLKLKVIAEGVETRDQLDFLQAHGCDEMQGFLFS
                     RAVSAEECARMLQEKRRLSASKRRGHASRGERRQPAQAGRG"
     gene            13329..13520
                     /locus_tag="VI319_09285"
     CDS             13329..13520
                     /locus_tag="VI319_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864081.1"
                     /translation="MSDEARVVVVDVKIHFWSMVILMVKWAIAAIPAFIILVALFWAA
                     SVVMGLLPFHWFGSGRGVV"
     gene            complement(13517..14137)
                     /locus_tag="VI319_09290"
     CDS             complement(13517..14137)
                     /locus_tag="VI319_09290"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY6864082.1"
                     /translation="MFYALPPATRWLLLANVAFFLVESLSGASGLVSYLALWPLGTAG
                     AVSFEPWQIVTYSFLHASFWHLFVNMFALYMFGGELERLWGQRRFVQLYFASVVAAAV
                     SQLVFASVSGAPPYPTVGASGGIFGLLLAYAMYFPHRTIVLLIPPIPMPAWLFVTLYG
                     LLELYLGVTGTQEGVAHIAHLGGMLGAWILILYWRGRLPFGTRRRR"
     gene            complement(14154..14229)
                     /locus_tag="VI319_09295"
     tRNA            complement(14154..14229)
                     /locus_tag="VI319_09295"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14194..14196),aa:Lys,seq:ttt)
     gene            complement(14269..15375)
                     /locus_tag="VI319_09300"
     CDS             complement(14269..15375)
                     /locus_tag="VI319_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY6864083.1"
                     /translation="MRATDPAALASLVIWGGFALAFVFGAVGNKTRFCTMGAVSDIVG
                     MGDWNRMRMWLLAIAVAILGASALQLAGLIDVSKAIYTTPNFTWLSYAVGGFLFGVGM
                     TLGSGCGSRTLIRIGGGNLKSLVVAIILAITAYMTLKGVLGVLRVAALDSVRATLATP
                     QDLPSLAARTLGIDKRTALAALALVLGGGLLAFVFRAREFRTFDNVLGGLVVGLVVVG
                     GWYLTGHLGYLAEDPTTLEERFVATSSGRMESLSFVAPQAYLLELLMLWSDKSRVVTF
                     TIAIAVGMVAGSFAWALVSRTFRLESFRDSEDLLSHIAGGALMGFGGVLALGCTIGQG
                     ISGVSTLAIGSFIALFGIISGAVVTMRWQYSRAQ"
     gene            complement(15380..16153)
                     /gene="ybgF"
                     /locus_tag="VI319_09305"
     CDS             complement(15380..16153)
                     /gene="ybgF"
                     /locus_tag="VI319_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY6864084.1"
                     /translation="MPRVRVSHVLLALAFAAPAAHAGLFDDEEARRQVANIRAQIDAN
                     QKALDERLKRLEAMGQERSDKTAVDLLQLIESMKQDLSKLRGQIEVLSNQTDQLERRQ
                     KDLYVDLDNRLRKMEQSNAQLQDKLNQGERDAQAETKAYETALSQFKLGNYQLSITGF
                     QNFLATYPNSSLAPNAQYWIGNAHYAMRDYKAAIAAQQKVVSSWPDHPKAPDALLDIA
                     SYQAEMGDEKAARQSLNTLVKKYPTSPAAEKARQRLVRK"
     gene            complement(16153..16701)
                     /gene="pal"
                     /locus_tag="VI319_09310"
     CDS             complement(16153..16701)
                     /gene="pal"
                     /locus_tag="VI319_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY6864085.1"
                     /translation="MNKSFVAACAVVALAVLYGCASSDVKPEGKPGVEERSPGAGGTQ
                     PGGPIVVEPVKPSDVTGNPLKDPKNILSKRSIYYDYDKDDIKDEYRPMVQAHARYLSQ
                     NAGAKMLIQGNCDERGSREYNIALGQRRADAVKRMLVLLGAKDSQIEATSLGEEKPRC
                     TAKTEECFAQNRRSDMLYSGEY"
     gene            complement(16819..18102)
                     /gene="tolB"
                     /locus_tag="VI319_09315"
     CDS             complement(16819..18102)
                     /gene="tolB"
                     /locus_tag="VI319_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY6864086.1"
                     /translation="MTRISRILAVLCLLAAGSARAQLTVEITGAGANQIPIGIVPFQA
                     EQALPQMVTPIVEADLARSGRFRTTYAGGTNPPPAESSQVNFAEWKARAVDALVIGST
                     RRLADGRFEVRFRLLDVPKEAQLAGFAFTLTAAQLRLTAHRIADIVYEKLTGERGVFS
                     TKIAYVVKQESRFALQIADADGFGAQSILISPEPILSPTWSPDGTRIAYVSLQHKKPV
                     IYVQSLTASRQPAPIANYRGSNSAPAWSPDGKQLAVVLSKDGPSQIYLMNPDGSNVRR
                     ITYSGAIDTEPFFSPDGQFIYFTSDRGGSPQVYRMPAQGGDATRLTFEGSYNVTPRVS
                     PDGKALAYIARVEGQFRLALMDLASRQTQLLTDGPRDESPSFAPNGRMLLYATDVGGR
                     GVLAAVSSDGRVQQRLTVQTADIREPAWGPFFGNP"
     gene            complement(18128..18892)
                     /locus_tag="VI319_09320"
     CDS             complement(18128..18892)
                     /locus_tag="VI319_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY6864087.1"
                     /translation="MNPALKVEEPGGKASAVLAVLVHVLLIAFLIVGVRWQSSKPEAV
                     VVELWDRAPVLEEPAPPPPPPPQVEPKPEPKIEPKPERPVEPKVEPKPVKPDIAVEKQ
                     KKPPKKEPPKKEQPKKEPPKKEPPLKLDPKQRIDEELAREMEALKQPKNPSPVTRPAG
                     PPPSLDAGYAAKLRGRIKSNIVLPQNIEGNPEAQFSIVQLPTGEVLSVTLTKSSGNKL
                     LDDAIERAIRKSSPLPLPDRPEQFQRELRIKYSPLD"
     gene            complement(18889..19302)
                     /locus_tag="VI319_09325"
     CDS             complement(18889..19302)
                     /locus_tag="VI319_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEY6864088.1"
                     /translation="MRPRKLVNQINIVPYVDVMLVLLVIFMVTAPLVNPAAIDLPSVG
                     KSSQPPPAPLEVIIRADQTMMLRERDKGRTDERPVSMQQLIAVVRERQRANPNVPVVI
                     AGDKNVRYEAVLKVMDELQKGNVRRVGLLVKPTGT"
     gene            complement(19299..20006)
                     /gene="tolQ"
                     /locus_tag="VI319_09330"
     CDS             complement(19299..20006)
                     /gene="tolQ"
                     /locus_tag="VI319_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY6864089.1"
                     /translation="MNVSQDLSILELVWHASAIVQAVMALLLVVSFMSWYFIFRKVFT
                     IRRAKRQTDQFERDFWSGSDLNALYQSAANHRHTIGSLERIFEAGFREFAKLRTQRAG
                     DPSAMVDGARRAMRATFQREMDRLEAHLAFLASVGSVSPYVGLLGTVWGIMHAFRGLS
                     NVVQATLAAVAPGIAEALVATAIGLFAAIPAVVAYNRFSHDVDRLSVRFESFMEEFSN
                     ILQRSAHSRHDTTKVPA"
     gene            complement(20003..20473)
                     /gene="ybgC"
                     /locus_tag="VI319_09335"
     CDS             complement(20003..20473)
                     /gene="ybgC"
                     /locus_tag="VI319_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEY6864090.1"
                     /translation="MSTKRAATVRRRPPPAASGDDDARAFSWPMRVYYQHTDAGGMVF
                     HANYLSFMECARTELLHALGFDLGELARRDGVLFVVHSAQIAYHKPALLHDALSVTAR
                     VARLGRARMEFHQSVVRGGEVLASADLTLVCVDARTHKPIGVPEAIREKLEGLQ"
     gene            complement(20481..21521)
                     /gene="ruvB"
                     /locus_tag="VI319_09340"
     CDS             complement(20481..21521)
                     /gene="ruvB"
                     /locus_tag="VI319_09340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY6864091.1"
                     /translation="MSIETDRLISGAGAAQEDAFERALRPKSLAEYVGQEKIRGQLAI
                     FVEAARNRKEALDHVLLFGPPGLGKTTLAHIVAREMGVNLRHTSGPVLERPGDLAAIL
                     TNLEPNDVLFIDEIHRLSPVVEEILYPALEDYQIDLMIGEGPAARSVKLDLPPFTLVG
                     ATTRAGMLTNPLRDRFGIVARLEFYSDAELQKIVNRSAALLAVEISPDGALEIAKRAR
                     GTPRVANRLLRRVRDYAQVRAQGAISREVADAGLRMLDVDPLGLDLMDRKLLAAVIEK
                     FAGGPVGLDNLAHAIGEEADTIEDVLEPYLIQQGYLQRTPRGRIATVSAYRHLGFAAP
                     ASGAGGADLFAK"
     gene            complement(21545..>21678)
                     /locus_tag="VI319_09345"
     CDS             complement(21545..>21678)
                     /locus_tag="VI319_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RuvA C-terminal domain-containing protein"
                     /protein_id="HEY6864092.1"
                     /translation="DVVNALLALGYSDKEAAQAVKQLPEGLSLSESIRQALKSLSKA"
BASE COUNT         3411 a         7418 c         7284 g         3565 t
ORIGIN      
        1 cagccgggat agccgggtca cgttgtgcgc gagtcgaggg gcggtcccgt ccatcgcgct
       61 cagggcatgt acgcgccgcc gttgacgtgg atcgcctggc cggtgatgta gcgcgagtcc
      121 gggccgcaca gcagccgcac gacggccgcg atctcatcgg gctcgccgcg cctgccgacg
      181 ggcggaaggc cgcgccggtg ctcggggcgc tcgggcgcgc cgggcagccc gcgcaccgtc
      241 tcgatcagcc ccggcaccac gcagttcacg gtgatccctt gcggagcgag gtcgagcgcg
      301 agcgcgcgcg tcattccggc gagcccggac ttggcggcga tcacatgcgc gcggccttcg
      361 gcgcccttgt gtgcggtcat gccgccgata ttgaccacgg cgcccccgcc cgcgcgcgcg
      421 atgtgcggca ggcacgcctg cgtgcacagg aacgccccgt cgagcacggt cgcgatcacg
      481 cggcgccact cctcgagcgt catgtcggcg aacggcgtct cggcgcgcac ggcggcgttg
      541 ttcacgagca cgtcgatgcg gccgaacttt ttcaccgtgg cgtcgatcat cgccttcacg
      601 gccttgccgt cggtcacgtc ggcgatgtgc acggccgcac ggccgccttc gccctcgatc
      661 atcgagaccg tctgctcggc gtcggcgcgc gacgtgttgg cgttcaccat caccgctgcg
      721 cccgcgtggg cgagcgcgcg actgatcgcg cggccgatgt tgcgggcgcc gccggtcacc
      781 agcgccacct tgccgtcgag ctccttgttc gtcgacatcg ctttccccgc caatgggctc
      841 caggcgaata ttaccaagag cgaatatacg ccgccggacc gtggcttcgt gcacgcccct
      901 gcggtctgct ttagcataac cgggagctcg tccccatccc tcgcggagac cccatgatcg
      961 tcgccggaga acgcgtccgc gtcttttggc agcccggatg caccagctgc ctgcgtacca
     1021 aggaattcct gaccaagaac ggcgtggact acgagtcgat caacgtccac ggcaatccag
     1081 ccggcatgga ggcgctgcgc aagctcggcg cccgcagcgt ccccgtcgtg gcacgcggcg
     1141 acaagttcgt gtttgcccag accctcaccg acgtgatcaa gttcctgggc ctgaagatca
     1201 ggctgcagga gcggctctcg cccgacgact tgatcgcgaa gctcaacgtc gtgctgccag
     1261 cggcggcgcg gtacgtgcgc cagatcccgg ccgactggct ggagaagtcc ttccgcaacc
     1321 gcaagcgccc gatccgcgtg ctcgcgcacc acgtgttccg cattcccgac ggcttcctgg
     1381 aatcgatgcg cgacggccgc gagctgacct acgagcgaat catggaagaa cccggcccgg
     1441 cgatccgcac cgcggatgac atcgcgcgct acggcgagtc ggtgctcggt cgcttcaacg
     1501 actggtggaa ggctttcccg gacaagtcgt gcgaaaagcc gatgcgcacc tacttcggcg
     1561 agcatccgct gcacgtggtg ctcgagcgca ccgtgtggca tccggcgcag cacacgcgcc
     1621 agctgatgct gatcctcgac accatcggca tcgagcccga tcgcaggctc acccccgggg
     1681 atctcgccgg gctgccgcta ccggacaagg cctgggacga cgagaaggag gcatcgtgag
     1741 gatcctgctg gtctgaccag cagcgttcac cagcgctggc gatggcgata ccagggctgg
     1801 accgggattt tccgcttcgg actcttgtcg tccatggtcg cggtgctcgc gaccgcctcg
     1861 gtcgtggcgg gctcggggga tttcgcgacg gcggtcgcgg gtcgggccgg ttgcgcgggt
     1921 tttttcttct cgggtttctt ctgctcggcc ttcttcttgg gggcttgcga cgccgtctcg
     1981 ggctcgccgt gcagcagggc gccgcggagc ttgtcgatgc cgcgcgacca gaagccgggc
     2041 ttggaacggg ccatggttct tgtgatgatc ccgcagtgac tcccctgagc attctattac
     2101 aatcgccgcc tccatgaagc ttcagctcag catcggcatg gcgtccaacc cgaggacctg
     2161 gccgatcttc gacgggcgcg tgcagcccga cggcatcgag ctcgtcgcga gcgtcgtgca
     2221 tcccagcgag ctcttctggc gacagctgaa attcgcggac ttcgacgtgt ccgagatgtc
     2281 gttctccacg ctgatgatgg cgagagccca gggcgacgac cgctgggtcg ggctgccggt
     2341 gttcacgacc cgcaagctct tccataccga gatcctggtg cggcgcgcgg cgggcatcga
     2401 gcgtcccgag gacctgcgcg gcaagcgcgt cgccgtgccg gagtaccagc agacggccgc
     2461 gctgtggacc cgcggagcac tgcagcacga gttcggcgtg gagccgaaag acatggagtt
     2521 ctggatggag cgcgtgccga cgcacagcca tcgcggcgca atcggcttca ccgcgccgcc
     2581 gggggtgacg atccggcaga tccccccgga gaagagcatc ggcggcatga tgctcgcggg
     2641 cgaggtcgac gcggtgatcc actacatcgt gaacgcgaat ctcgtcgacc gcagcaccgt
     2701 ggacctgtgg agccatccgg agatcaagcc gctgtttccc gacccggtcg ccgagggcgt
     2761 tcgctactac cgcaagaccg gcctgctgcc gatcaaccac ggcatggtga tacggcgcga
     2821 gctcgtcgag cgccatccct gggtggtgct gaacctgctg aaggctttcg aacgcgccag
     2881 cgagatcgcc gagcgcgagc gcctcgagca cgccgcctac cacgtcgcga cgggcctcgt
     2941 gccgcccgaa ggactgcaga gcctgcgcac gaccgtggtc cgccatggca tcaaggcgaa
     3001 ccgcaaggtg ctggagaccg ccgcgcaata ctcgctcgag cagggactca cgtcacgccg
     3061 ggtcggtgtc gaagaagtct tcgcgccgag cacgctcgag cagtgacgcg cgcgcccggc
     3121 atggtttctt gccgctacac tgtctgctgc gaattccacc cagtccgata cgaggtttcc
     3181 gcatgacgag cttccgcaac tttccgaccg gcgccgccct cctcgcggcg ctcgtggcgt
     3241 tcgcccccgt cgccgtgcgc gcgcaggccg acgcctatcc gagcaagccg atccggttca
     3301 tcctgccgtt cccgccgggc ggcggtaccg acatcctggg gcggctcctg gccgagcggc
     3361 tcgcgcggag gctcgcgcag ccggtggtca tcgagaaccg cgggggcgcc ggcggcaacg
     3421 tcggcgccga ggccgcggcc cgctcggcgc ccgacggtta cacgatcgtc ctggtcgcgc
     3481 cgtcgctcgc catcagcccg agcctgtaca agaagctcgc ctacgatccg gtgaaggact
     3541 tctcaccggt gagcctcgtc gccaccgtgc cgaacgtgat ggtgaccaac ccgtcgatac
     3601 ccgcgaagac gctctccgag ttcatcgctc tcgcgaagag caagcccggg gcgatgaact
     3661 tcggctcggg cggcaacggg acgtcgaacc acctcgccgg cgagctgttc aacatcgtcg
     3721 ccggcgtgcg cctggtgcac gtgccgtaca agggcgtgaa cctcgcgatg aacgacgtca
     3781 tgtcggggga cgtgcagctg gtaatcatcg ggatccccgc ggcggcaccg cacatcaagg
     3841 cgggcaagtt gcgcgcgctc gcggtgatcg actccaagcg ggccgcggcg ctgcccgacg
     3901 tcccgacggc ggccgaagcg gggctgaagg acttcgacgt gaccacctgg tacggcgtgc
     3961 tggcgccggc cggaacaccc cgaccgatcg tgacgcgtct gaacgcggag atcgtcgccg
     4021 tcatgaactc gagcgagatg aaagagcggc tcgcgacaat ggcgaccgag cccgtgacca
     4081 gcacgcccga ggagttcgga gacctgatcc ggagcgagat cgtgaaatgg gccaaggtcg
     4141 tgcgcgccgc gggactcaag gccgactgac cgtgtacctg cccgcgcact tcgaagagcg
     4201 cagcgtggag acgctgcacg cgctgatccg cgagcatccg ctgggcgccc tgatcacgct
     4261 gggaccggac ggcctcaccg ccaatcacgt gccgttcgag atcgatccgg accccgcacc
     4321 gttcgggacc ctgcgcgccc acgtcgcgcg cgcgaacccg gtgtggcgcg agcatccgcg
     4381 ggacgcggac gccctcgtcg ttttccaggc cgagtcgggc tatgtgtcgc cgtcctggta
     4441 tccgaccaag cgcgacacgg gcaaggtggt ccctacctat aactatgtcg tcgtgcacgc
     4501 ccggggcacg gttcgggcga tcgaggatcc ggggtggctg cgcggcctgg tgagccggct
     4561 gaccgaccgt cacgaggcgg aaagcgggca tccctggaag gtgaccgacg cgcccgagga
     4621 tttcatcgag agccagttgc gcgcgatcgt cggcatcgag atccggccgc acagtctgat
     4681 cggcaagtgg aaggtaagcc agaaccggcc cgcagtcgac cgcgaaggcg tggtgcgagc
     4741 gctcgaggat gctggcgacg cggggtcgct cgacttggcg cggcgcgtcc aggacgcgct
     4801 gcgcgcgaaa ccgggaaggg tttagtaggg gcgtgtcgtc gagcgccggg tgtagacgat
     4861 ccccggtgcc acgacccgct cgccgaccgc gcgccgcaac gcctgcggcg ggcagatccg
     4921 gtcgaactcg tagtcgatgc cgtcgaaacg cgcgctgcgg tggatgggca cgcccatgta
     4981 ccagccgatg gcctcggagc tcgcttcggg tgagtcgaac aggaacggcg gcggtgcgac
     5041 cggctcctcg atcttcggct cgggttcggg ccgcggcgcc gggtcgcgcc gcacggtgct
     5101 cagggccgcg gacgcgagca tcgacaagcc gacccagagg acgggcagca gccaccagga
     5161 gttcagcacc gagtcggtcg cgctcatgtt cgttttaggg gccctcccgc aggcccggag
     5221 accgtcgcga gccgactgtc aagcgccatt cggccgcgcg ctgtttttga aacgctatta
     5281 caaatcagaa tcttatagaa ccgcttgaca gcctgagggg gggttgcctc atgctgttgg
     5341 ggcaatctct cttctgtacc gaatgtctgc ggatcaaccc gagcctgtaa ccccgggctg
     5401 tccgcagcat tcgtacccgc tagggcctcg agttggtccg gaaagatcca ggctgcatgt
     5461 tccaccgcag cctggacgct tccccgaaac cgctcttggc ccttcttttt aggcttcttc
     5521 gtcgccgcct cgactggcgg acttgttctt ggctctttcg cgggattgct tgcttcctta
     5581 cttcttcttc ttagcggatt tctttttcgc ttttttcttt gccatggcaa tctccttcat
     5641 gagttaaaca cacaagcaac ccgacccgtc catgtcggca cgggccattc aaccgcgcgg
     5701 gacaaagccc gtgcgagtga atccctcgtc cgcccgccga agcggcgagc aagatcgatg
     5761 cggtgatttt ttttcttgga ggaaggatgc ggaccgctga cttcgtcagc agaaagtccg
     5821 tgtccaagac gaacgtcgct cggcgcgccg gcgacgcgac tgtgagcgct aggcaaaaag
     5881 ttgggctgca agaacaaaca tggcgcaatt aatactacat tgaagttcac aaagcaagac
     5941 cttcgacgca aatttgcgcc gatcatcatg catttgaggt ccgaaaaggc atccgaaacg
     6001 cgcgatcacg cgtcgaagac gtgttccgag acgctgcaat cgctcgaagc gagcgaatcg
     6061 actaccgcaa cgcgtcggat ccgagcaatg cttcccggaa tctctgcttg agatagccac
     6121 cgtcgcgcgg cggcagggca ggcgccgacg cgcccgccgc gactttcacc tggaagaaaa
     6181 gcggcccgcg cgcttccagc actgcggtgc gcatcgatgc gatctccccg ggcgcggaaa
     6241 cgccgcgggc ggccgagaaa ccgcacgccc gcgccacggc cgccaggtcg acgccgtgcg
     6301 ccgtgtgcgt cttctgcatc ccggtctcgc cgtagcgctc gttgtcgagc acgacgagtg
     6361 aaaggttgcg cggccgctgc gcgccgatgg tggcgaggct gcctagtccc atcagcatct
     6421 cgccatcccc ggtgaccacg agcacgcggc gcccgggctg cgcgatcgcg agacccatgc
     6481 cgaccatgca ggcggagccc atggcacccc aaagcggaaa ggtgagcgga tgatcgcccg
     6541 cggccgcgca atcccaggcc ggcgcaccga gtcccgccac gacgaggagc gcctcgcgcc
     6601 gctcgagcag cgccgcgacc accgcacggc gatcgagcgc cgcgctcacc gcgcacccgc
     6661 gaaggacttc gcgcctagca tcttctgaga gacgaggacg gccgccatgg catatccgtc
     6721 gaacgcgagg gccgccgcgg cgtcgaccgt cgcggctgca tcctcggggc gctcgagacg
     6781 aaaggtgcgc acgccgctcg cgtgcaagat tggctcggtc gcctgtccca tgggaatctg
     6841 ccaggggttg aattcgcccc attcaccgcg catggtgacg atcagcagca gcgggaaccc
     6901 gcagttcgac ccgagcgtga gcgcgttcac gcaatttccg acgccgctcg actgcatgag
     6961 cagcgcggcg cgttgcccgc ccagccaggc gcccgcggcg aggccgatgc cctgctcctc
     7021 gctcgtcagc ggaaccgtga cgatcgatcg gtcggcgtgg cacagctcga tgagaagccg
     7081 atggcccgcg tcggggacat aggccacttg ccccacgccc cgagacttca atacgtcatg
     7141 aacggcggcc ggccaggccg cctcggtgga cgagcgccga ttccgattgg acaaggggcc
     7201 ccgtggcagt gttgtaagga agcgcgcgca ttgtaccgtc cagctctcac cacccgcacg
     7261 agacaccatg cgaatcctga tctgtctgct caccgccgcc gccgcgctgg tctgcgcgca
     7321 ggcacgcgcc cagaacatct acaagtgggt cgaccgcaac ggccgaatcc actactcgga
     7381 cgtggtgccc ggcaaggatt cgaaagcgat cgccatccag gaccgtctgt cgatttactc
     7441 ccccgagccc gccgtcgctc aggcgctcca gctcaccccg aggagcagcc agaccgtggc
     7501 cctcgccgat cgggtggcga cgctggagcg ccagctgcaa gccgagcgcg ccgctcggca
     7561 accggccccg ccggacacca gcgcagcata cgagcgttgc ctcgcggatc ggcgcacgga
     7621 ctgcgatcaa ctgctgagcg gcgccgcggc tccgggcgtc gacgcgccca ccgctttccg
     7681 cccgggacca gcgacgctgc gcagggcgag cgcgtcctag ccgcgccccg cctgcgcggg
     7741 ctgccgccgc tcgccgcgtg aggcgtgacc tcggcgcttc gacgcgctca gccgccgctt
     7801 ttcctggagc atgcgcgcgc attcctccgc ggatacggcg cgggagaaca ggaacccctg
     7861 catctcgtcg cagccgtggg cctggaggaa gtcgagctgg tcgcgcgtct cgacgccctc
     7921 cgcgatgacc ttgagcttca gactgtgcgc caggccgatg atggcgaagg tgatagcggc
     7981 gtcgtccggg tctacggtga cgtcgcgtat gaaggcgcga tcgatcttga gggcgtcgat
     8041 cggaaagcgc ttcagatacg agaggctgga gtagccggtc ccgaagtcgt cgaccgatag
     8101 cgtcacgccg agctccttca ggccgcgtag cgtccgcgcc gccacctccg gctccttcat
     8161 caatagggat tcggtcagct cgaactggag cagcttcggg tcgatgtcgc tcgcgcgcaa
     8221 cgaggcggcg acggtctcct ccagcccctt ctgctggaac tggcgcgccg agaggttcac
     8281 ggcgaccggg ggcacgcgga agccctcacg gctccagcgg gcgatctgct cgcacacctc
     8341 cgcgatcacc cagtcgccga cggtcacgat gagcccggtg tcctcgagga ccgggatgaa
     8401 ctggaggggc ggaaccagtc cgctctcggg gtgctgccag cgcagcagcg cctcgaaccc
     8461 gcacacggcg ccggtggtga tgctgacctt tggctgatag tgcaggagga attcgcgtcg
     8521 ctcgatggcg cggcgcagcg ccgactccat ggtcacccgt tgcatcgcgc gctcgttcat
     8581 ctcgcgcgtg aagtactggt aggtgttgcg gccctgctcc ttcgcgcggt acatggccgc
     8641 gtcggcgttc atgatcagcg taccggcctc ctccccgtcg gtcgggaaca gcgtgatgcc
     8701 gacgctcgcg gtcacgtagc tctcgtggcc gtcgagattg aagggacgcg cgagcgcgtc
     8761 gatgatcttc tgcgccacca cgctggcgtc tccgggcttc gcgaggtcgg tgatgatggc
     8821 gccgaactcg tcgcccccga accggccgac ggtatcgctc gagcgcacgc aggcctgcag
     8881 gcgctcggtc gcatccttga gcagccgatc gccggcgccg tgtcccagcg tgtcgttgat
     8941 cagcttgaat cggtcgaggt cgatgaagag gaccgccatc gctttgctgc tgcgccgggc
     9001 ccgcgccatc gcctgcatca ggcggtcgcg gaagagatga cggttcggaa gtcccgtcag
     9061 ggagtcgaac tgcgccagat acgcgagctt ctcctcggcc tccttgcgca tggtgatgtc
     9121 ggtcatggcg ccgatcatac ggaccgcctt gcccgccgag tcgcggaccg cgtgcccccg
     9181 gtccagcacg cgcgcgtacg tgccgtcggc gcgcaggaag cggtattcgt cgctccagtt
     9241 gtcgcccccg gactcgagca gctcgcggat tcccttcacg atgcggtcgt ggtcctccgg
     9301 atggatgtgg cgcttccaga actcgccggt acggctggcc tcgggggcgt agccgaacac
     9361 gcgcttcagg ttctcgttcc accacatctc gtccgtcgtg agatcccagt cccagatcac
     9421 gtcgttggtc gcgcgcaccg cgaggttgaa gcgctcgttg ctccggcgca gggcttcttc
     9481 cgcggcgagc cgctcgcgga tgtcgcgcga gacggcggcg atgatgtact tgtcgcccga
     9541 gcgcagcacc cggcgggtcg actcgaacgg gaggtgcgtg ccgtcccggc agcggtacac
     9601 cgtgttcatg ccgctcgggc gcgacggatc ggcgatcagc gcgtcgtacg accgctcgag
     9661 ctcctcgcgc ccgaccggca gcacgtcgtg cgggcccatc tgcaggagct cctcgcggct
     9721 gtagccgagc agccggcatg cggtcctgtt cacgtcgatg aagcgcatgg tggcgcggtc
     9781 gatcagcacg atcatgtcgg ccgagttgtc catcgcgagc cggaaccgct gcagctcctc
     9841 ctcgcgctcg aggcgctcgg tgacgtcgcg cgcggccgcg acgatgacat agccgtctcc
     9901 cgaaggcacg acgcggcgca tcgattccat gggcacgagc gcaccgtcct tgcgccggta
     9961 cgagcccgcg atcaccatct ccttgccgtc cccggcgatg agccgccggt aggtctcggc
    10021 aagttcggcg cgcggagccg acaggatgtc gtgcggtccg agcgcgagca gctcctcgcg
    10081 cgtgtacccg agcgtccggc aggcggtgtc gttcacgtcg acgatgcgca tgccgatcgg
    10141 gtcgatgagc cagaacatgt cggccgacgc gtccatggcg gcccggaagc gctggagatc
    10201 ctcctcgctt tgcttgcgct gcacgaactg tccgatctgc cggccgatga cgtggatcgc
    10261 ctggagcaag cgctcctcgg gctcgcgcgc ctggcggctc gagaaattca gcacgccgat
    10321 cgtcttgccg tccgcgacca ccgggaacac gaacgagccg tggatcccga gcgcgctcgc
    10381 gaccgagcgc ttgccctgga cggtgagtga gcgcgggtcc ttggtcacat cggtcgacca
    10441 gagcggttgg cccgactgcc aggcgcagcc ggacaggcct tcgccgggac gataggcgag
    10501 cttgcgcgcc gactcgatga agctctggat cgcgggatcg tcgacacccc atgcctcggc
    10561 gaagcgcaga acctgcccct cttcgtcgac ggcgaagtaa cgcccgcaat cccatccctg
    10621 cgactcgcac acggtccgga tgaccgcctt cacgcaggcg tggacttcat cggcatcggc
    10681 cagatcgcgg ttgaccgcgt gctccagggc gcgcagccgt tcctcgaact tgcgttcggt
    10741 gatgtccttg ccgatgcccc ggtagccttt gaaatttccc gagccgtcga agatcggctc
    10801 gccgctgatc agcatccagc tcggcctgcc gtcctcggca agtcggcaca gctcgaggtc
    10861 gtggaagggc tggtgcgcct ccacgatcgc gcggtgcgcc gcccagtcgg cctggctcat
    10921 gttgagcgcg ggcagatccc agcgcagctt gccgatgtgg ctggcggcgc tcaccccggt
    10981 cttgccgaga atgtcctcgt tcatggcgac gaagcgcagg ttctcgtcct gctcccagta
    11041 ccagtcggag gaaagtttcg tcaggctgcg gaagcgcgct tcgctcccgc gcagcacttc
    11101 ctcggcctcc ttgcgctgca cgaactggcc gatctggctg cccaccacgc ggatcgcctg
    11161 tagcagccgc tcgtcgggct ggcgaacgtc gtgactcgcg aagccgagca cgccgatgac
    11221 ctcgccgccc gcggtcacgg ggaacacgaa agccccgtgc atgttgaacg gacgggcgac
    11281 gaccatggtg acgcgcgcgt ccttcgtgac atccggcacc cagaggggcg tgcgtgtctg
    11341 ccacgcgatg cccgcgagcc cgacgccggg cgcaaacgcg agctcgcgcg atctctcgat
    11401 gaactcgcgc atcgcgtcgc cctccacgcc ccacccctgc tggaagcgca gcatctgact
    11461 tctgccgtcg acgcgccagt agcgcccgca ctcccagctc tcggcctcgc aaatcgcgcg
    11521 gatcaccgcc tcgagcgcgc tcggaacgtc ctcggcggtc gcgagacacc ggttcacccg
    11581 gtgctcgagc tcgaggagcc gttcgtggcg cttctcctgg gtgacgtcct tcgtgatgcc
    11641 gcgatagccc ctgaatccac ccgcgccgtc gtagaccggg tgtccgcgga cattgaggta
    11701 gacgactcgc ccgccccgcc ccgtcgcttt gagcacgatg tcccggaaca gcgagcgcga
    11761 ctcgagcagc tcgcgatagc gccgccagtc cgcgtcggcc ggatcgacgt acggcatctc
    11821 ccagggccgc tttccgaggt gcttccgcgc ctgctctccg gcacgcggcg agggcttgtt
    11881 cggcgtgagg gcgagcatac ggaatttctc gtcgagctcc cagtaccagt ctgcggacag
    11941 gcccgtcaac gcgcggtaac ggttctcgct ctcgcgcaga tccttttcgg cgagcgccag
    12001 ccgattcaga cgcccgagcg tcaccgtaac gagcagcagc agcacgagcg agagcagcgt
    12061 gcctccggcg agataggcgc gccgctgctc gtagtaccgg gcgagcgttc cctgcagctc
    12121 ctggccggcg accacgacga gcggatagcc ggacagcctg cgatagctgt agatgcgctc
    12181 gatcccgtcc ctgggcgagc gccggatgag gctgcccgtc gccgggcccg acgcgtccag
    12241 ctcctgccac tccgggcgcg gtcccccgat caggctctcg aagtcggaag accgagccac
    12301 gaccgtgccg tcgtggtgcc gcagcgtgat gaacccgccc ggaccgaggt cgatcgcctt
    12361 gtacgcgcgc accaggtcgc cggcggagat ggcggcggag acgatgcccc ggaactcgcc
    12421 ggcactcctg atcgcgagcg cgaacgggat gaagattctc gcgtcgccgg ggacgcgctc
    12481 ggggttgccg acgaggagct gttccacgcc gccggcacgc gccgcggcga agtagtcggt
    12541 ctcggccagc gagcgccccg gccgggcagg atacgcgaca agcccttccg cgtccgtgac
    12601 cgcgagccga tcgaggatgt ggcctgacag cgaaatatgc tcgttgacgg tggccgaaaa
    12661 gcggcgcgga tttctcagcc actgttcccg caaatgcctc aacgtgccgt cgatgctgcc
    12721 cgcgaacacc aggaaatgct cgcccaccac cgcggtgatg ttctccccgt gccggcgcgc
    12781 ggtatcgatc gcctcgctgc gcagctggtg cagctggtag gcgacggcgg tccagacggc
    12841 gatcaagacg acgcaagcgc cggccaacag cggcacgcga agccgatgga tcggtgcgcg
    12901 gttcatggtc ctgtcccgag cctccctgct cggcacgctt gtacgggatc ggggtagacg
    12961 aactcatgca agcgtcatgc ccgcccgcgt ctgaagatag ccgcccgtgg gctgccgaat
    13021 ccatagggaa aatcccgaaa cctcggggaa ctcctgactc cggcgcagcg cgcggtgtcg
    13081 gacactcccc atagcgtcgg acggccgcca ctgcgctgcc gccgcaccgg gattaagctc
    13141 gtgactcccg cctgcgatcc cgtgccggac gtactcgccc tcatcggcaa tatctctcga
    13201 tccgcgtcac gcggatcgat acgggtcgac atcctagccg acttggatca ggctccgcgc
    13261 ggcagacgcc ggacgcgaat gggccgtcgg ccgcttagaa tgcgccggac gaaaacaacg
    13321 gaggctctat gagcgacgaa gcgcgcgtgg tggtggtgga cgtcaagatc cacttctggt
    13381 cgatggtcat cctgatggtg aagtgggcga tcgccgcgat ccccgcgttc atcatcctcg
    13441 tggcgctttt ctgggccgcg tcggtcgtaa tggggctgct gcccttccac tggttcgggt
    13501 cgggccgcgg cgtggtctag cgtcggcggc gcgttccgaa cggcagccgg ccgcgccagt
    13561 agaggatcag gatccacgcg cccagcatgc caccgaggtg cgcgatgtgc gcgacgccct
    13621 cctgcgtgcc ggtgacgccg agatagagct cgagcaggcc gtacagcgtc acgaacagcc
    13681 aggcgggcat cgggatcggc ggaatcagca gcacgatcgt ccggtgcgga aaatacatcg
    13741 cgtaggcgag caacagcccg aagatgccgc ccgaggcgcc caccgtcgga tacggcggcg
    13801 cccccgaaac gctcgcgaac acgagctggc tcaccgccgc cgcgacgacg ctggcgaagt
    13861 agagctggac gaagcgtcgc tgcccccaca ggcgctcgag ctcgccgccg aacatgtaca
    13921 gcgcgaacat gttgacgaac aggtgccaga agctggcgtg caggaagctg taggtgacga
    13981 tctgccacgg ctcgaacgag accgcccctg cagtgccgag cggccagagc gcgaggtagc
    14041 tcaccaaccc cgaggcaccg ctcagcgact cgaccaggaa gaacgcgacg ttcgcgagga
    14101 gcagccagcg ggtggcgggc gggagagcgt aaaacatcag attttggacc cggtggtagg
    14161 ccgtgcaggg ctcgaacctg cgaccaacgg attaaaagtc cgctgctcta ccaactgagc
    14221 taacgacccc cgaagagacg cgaaatttta gcacagcgag cgccgcgctc actgggcgcg
    14281 cgagtactgc catctcatcg tcacgaccgc gcccgagatg atgccgaaca aggcgatgaa
    14341 cgacccgatc gcgagcgtcg acacgcccga gatcccctgt ccgatcgtgc agccgagcgc
    14401 gagcacgccg ccgaatccca tcaatgcgcc gccggcgatg tggctcagca ggtcctcgga
    14461 gtcccggaag ctctcgaggc ggaaggtgcg cgacaccaac gcccaggcga acgagccggc
    14521 gaccatcccg accgcgatcg cgatcgtgaa ggtgacgacg cggctcttgt cgctccagag
    14581 catgagcagc tcgagcagat aggcctgggg cgcgacgaac gagagcgact ccatgcgccc
    14641 cgagctggtc gccacgaacc gctcctcgag ggtcgtcgga tcttcggcga gatagccgag
    14701 atggccggtg aggtaccagc cgccgaccac caccaggccg acgaccagcc cgcccagcac
    14761 gttgtcgaag gtgcggaact cgcgcgcccg gaacacgaag gcgagcagcc cgccgccgag
    14821 gacgagggcg agcgccgcga gcgcggtcct cttgtcgatc cccagcgtcc tcgcggcgag
    14881 cgagggcagg tcctgcggcg tcgcgagcgt cgcccgcacc gagtcgagcg ccgcgacgcg
    14941 cagcacgccg agcacgcctt tgagcgtcat ataagcggtg atcgcgagga tgatggcgac
    15001 gacgagcgac ttcaggttcc cgccgccgat gcggatcagc gtgcggctgc cgcagcccga
    15061 tccgagcgtc atgccgacgc cgaagaggaa gccgccgacg gcgtacgaga gccaggtgaa
    15121 gttcggggtc gtgtagatcg ccttcgagac gtcgatcagt ccggcgagct gcagggcgct
    15181 cgcgccgagg atcgcgaccg cgatcgcgag cagccacatg cgcatgcggt tccagtcgcc
    15241 catgccgacg atgtcggaca cggcgcccat cgtgcagaag cgggtcttgt tgccgacggc
    15301 gccgaagacg aacgcgagcg cgaagccgcc ccagatgacc agcgatgcca gcgcggcggg
    15361 gtcggtcgcc ctcaagcgcc tacttgcgca cgaggcgctg tctcgccttt tccgccgccg
    15421 ggctggtggg gtatttcttg accagcgtgt tgagcgactg gcgcgccgcc ttctcgtcac
    15481 ccatctcggc ctggtagctc gcgatgtcga gcagcgcgtc gggcgccttg ggatgatccg
    15541 gccacgagct cacgaccttc tgctgcgcgg cgatcgccgc cttgtagtcg cgcatcgcgt
    15601 aatgcgcgtt gccgatccag tactgcgcat tcggcgcgag cgagctgttc gggtaggtcg
    15661 cgaggaagtt ctggaaaccc gtgatcgaga gctggtaatt gccgagcttg aactggctga
    15721 gcgcggtctc gtacgccttc gtctccgcct gcgcgtcgcg ctcgccctgg ttgagcttgt
    15781 cctgcagctg cgcgttcgat tgctccatct tgcgcagacg gttgtcgaga tcgacgtaga
    15841 ggtccttctg gcgccgctcg agctggtcgg tctggttcga caggacctcg atctggccgc
    15901 gcagcttgct caggtcctgc ttcatggact cgatgagctg cagcaggtcc accgccgtct
    15961 tgtcggagcg ctcctgcccc atcgcctcga gccgcttcag gcgctcgtcg agcgccttct
    16021 ggttggcgtc gatctgcgcg cggatgttcg cgacctggcg gcgcgcctcc tcgtcgtcga
    16081 acagcccggc gtgagccgcc ggggcggcga aggccagcgc gagcaggacg tgggagacgc
    16141 gcacgcgggg cactagtatt ctccgctgta gagcatgtcg ctgcgccggt tctgcgcgaa
    16201 gcactcctcg gtcttcgccg tgcagcgcgg cttctcctcg ccgaggctcg tcgcctcgat
    16261 ctgcgaatcc ttcgccccga gcagcacgag catacgcttg accgcgtccg cgcgccgttg
    16321 cccgagcgcg atgttgtact cgcggctgcc gcgctcgtcg cagttgccct ggatgagcat
    16381 cttcgctccc gcattctgcg aaaggtagcg cgcgtgcgcc tgcaccatcg gacggtactc
    16441 gtccttgatg tcgtccttgt cgtagtcgta atagatcgag cgcttggaca ggatgttctt
    16501 cgggtccttg agcggattgc ccgtgacgtc cgaaggcttg accggctcga cgacgatggg
    16561 tccgcccggc tgggtcccgc ctgcccccgg gctgcgctcc tcgacgcccg gcttgccttc
    16621 gggcttcacg tccgagctcg cgcaaccgta cagaaccgcc agcgccacca cggcgcatgc
    16681 cgcaacgaat gatttgttca tgccgtattc tcctctagaa acgtccgatt tggccaatcg
    16741 ccccggccct cccgtcgatc tcgaggctct tgcatgctgt cgctctgcgg cgacgcgttc
    16801 tttccctgca tccctgcatc acgggttccc gaagaacggt ccccaagcgg gctcgcggat
    16861 gtcggctgtc tgcaccgtca gccgctgctg gacgcggccg tcgctcgaga ccgccgccag
    16921 cacgccgcgt ccgccgacgt cggtcgcgta cagcagcatg cggccgttcg gcgcgaagct
    16981 cggcgactcg tcgcgcgggc cgtcggtgag cagctgcgtc tggcggctcg cgaggtccat
    17041 gagcgccagc cggaactggc cctcgacgcg cgcgatgtac gcgagcgcct tgccgtcggg
    17101 gctcacgcgc ggcgtgacat tgtagctgcc ttcgaaggtg agacgggtgg cgtcgccgcc
    17161 ctgcgccggc atccggtaga cctgcgggct gccgccgcgg tccgacgtga agtagatgaa
    17221 ctgcccgtcg ggcgagaaga agggctcggt gtcgatcgcg cccgaatacg tgatgcgccg
    17281 gacattcgag ccgtcggggt tcatgaggta gatctgcgaa gggccgtcct tcgagagcac
    17341 caccgcgagc tgcttgccgt cgggcgacca ggcgggcgcg ctgttcgagc cgcgatagtt
    17401 cgcgatgggc gccggctgcc ggctggccgt caggctctgc acgtagatga cgggcttctt
    17461 gtgctgcagc gagacgtatg cgatccgcgt gccgtccggc gaccaggtgg gcgagaggat
    17521 cggctcgggc gagatcagga tcgactgcgc gccgaagccg tcggcgtccg cgatctgcag
    17581 cgcaaagcgc gactcctgct tcaccacgta cgcgatcttg gtgctgaaca cgccgcgctc
    17641 gcccgtcagc ttctcgtaga cgatgtccgc gatgcggtgc gcggtcagcc gcagctgggc
    17701 ggcggtcagc gtgaacgcga agcctgcgag ctgcgcctcc ttcggcacgt cgaggagcct
    17761 gaacctcact tcgaagcgtc cgtcggcgag ccggcgcgtg ctgccgatca cgagcgcgtc
    17821 caccgcgcgc gccttccact cggcgaagtt cacctgcgag ctctcggccg ggggcgggtt
    17881 cgtgccgccc gcgtacgtgg tgcggaagcg cccgctgcgc gcgaggtcgg cctcgacgat
    17941 cggcgtgacc atctgcggca gcgcctgctc ggcctgaaaa gggacgatcc cgatcgggat
    18001 ctggttcgcg ccggcgccgg tgatctccac cgtcagctgc gcccgggccg agcccgcggc
    18061 gagcaggcaa aggaccgcaa gaattctgga gattcgtgtc atgcgcggaa tggccggtta
    18121 gtgtagctca atcgagcggg ctgtacttga tgcgcagctc gcgctggaac tgttccggcc
    18181 ggtcgggcag cggcagcggg gacgactttc ggatggcgcg ctcgatggca tcgtcgagca
    18241 gcttgttgcc gctcgacttc gtcagtgtca ccgacaacac ctctccggtc ggaagctgca
    18301 cgatgctgaa ctgggcctcc gggttgccct cgatgttctg cggcagcacg atattcgact
    18361 tgatccgccc gcgcaatttc gccgcgtacc ccgcgtcgag cgaaggcggg ggccccgcgg
    18421 ggcgcgtcac cggcgaaggg ttcttcggct gcttgagcgc ttccatctcg cgtgcgagct
    18481 cttcgtcgat gcgttgtttc ggatccagct tcagcggagg ctccttcttc ggcggctcct
    18541 tcttcggctg ttcttttttc ggcggctctt tcttgggcgg cttcttttgc ttctcgaccg
    18601 cgatgtcggg cttgaccggc ttcggttcga ccttaggttc gactgggcgt tcgggcttgg
    18661 gttcgatttt gggctcgggc ttcggctcga cttgcggcgg cggtggcggt ggcggagccg
    18721 gttcttcgag cacgggggcg cgatcccaca actcgaccac caccgcctcg ggcttgctcg
    18781 actgccagcg cacgcccacg atcaggaagg cgatgagcag cacgtgcacc agcacggcca
    18841 ggacggccga ggctttcccg cccggctcct cgaccttcag cgccggattc atgttcccgt
    18901 gggtttgacg agcagcccca cccgccggac gttgcctttc tggagctcgt ccatgacctt
    18961 caggaccgcc tcgtagcgca cgttcttgtc gccggcgatg accaccggca cgttgggatt
    19021 cgcgcgctgt cgctcgcgca cgactgcgat gagctgctgc atcgacaccg ggcgctcgtc
    19081 ggtgcggccc ttgtcgcgct cgcgcagcat catggtctgg tcggcgcgga tgatcacctc
    19141 gagcggcgcg ggcggcggct gcgagctctt gccgacgctc ggcaggtcga tggccgcggg
    19201 attcacgagc ggcgcggtca ccatgaagat gacgagcagc accagcatca cgtccacgta
    19261 cgggacgatg ttgatctggt tgacgagctt gcgcggcctc acgccgggac cttggtggtg
    19321 tcgtggcgcg agtgcgcgct gcgctgcagg atgttcgaga actcctccat gaagctctcg
    19381 aagcgaaccg agagccggtc gacgtcgtgc gagaagcggt tgtaggcgac caccgccggg
    19441 atcgccgcga acaggccgat cgcggtggcg accagcgcct cggcgatccc gggcgcgacc
    19501 gcggccaacg tcgcctgcac cacgttcgac agcccgcgga aggcgtgcat gatgccccag
    19561 acggtgccga gcaggccgac gtacgggctg accgacccga ccgaggcgag gaacgcgagg
    19621 tgcgcctcga gccggtccat ctcgcgctgg aaggtcgcgc gcatcgcgcg gcgcgcgccg
    19681 tcgaccatcg cgctcggatc gcccgcgcgc tgggtcctca gctttgcgaa ctcgcggaag
    19741 ccggcctcga agatgcgctc gaggctgccg atcgtgtgcc ggtggttggc tgcgctctgg
    19801 tagagcgcgt tcaggtccga gccgctccag aagtcgcgct cgaactgatc ggtctggcgc
    19861 ttggcgcgcc ggatggtgaa caccttgcgg aagatgaagt accacgacat gaacgagacc
    19921 acgagcagca gcgccatcac ggcctgcacg atggcgctcg cgtgccacac gagctccagg
    19981 atggacagat cttgcgagac gttcattgca gtccttcgag tttttcccgg atcgcctccg
    20041 gtacgccgat cggcttgtgc gtccgcgcgt ccacgcatac gagcgtcagg tccgcgctgg
    20101 cgagcacctc cccgccgcgc acgaccgact ggtggaattc catccgggcc cggccgagcc
    20161 gggccacccg cgcggtgacc gagagggcgt cgtgcaggag cgcgggctta tggtaggcga
    20221 tttgcgccga atgcaccacg aacaggactc cgtcgcggcg cgccagttcg ccgagatcga
    20281 agccgagcgc gtgcaatagt tccgtccggg cgcactccat gaacgacagg tagttcgcat
    20341 gaaacaccat gccgcccgcg tcggtgtgct ggtaatagac gcgcatcggc caggaaaagg
    20401 ctctggcgtc gtcgtcgccc gacgctgccg gcggcggacg ccggcgcacc gttgccgcgc
    20461 gcttggtgct cacccggcgc tcatttcgcg aacaggtcgg cgccgccggc gccggacgcc
    20521 ggcgcggcga agccgaggtg gcggtacgcc gagaccgtcg cgatgcggcc gcgcggcgtg
    20581 cgctgcaggt agccttgctg gatgagataa ggctcgagca catcctcgat cgtgtcggct
    20641 tcctcaccga tggcgtgcgc gaggttgtcc aggccgaccg ggccgcccgc gaacttctcg
    20701 atgaccgcgg cgagcagctt gcggtccatg aggtcgaggc cgagcgggtc gacgtcgagc
    20761 atccgcaggc ccgcgtcggc gacctcgcgc gagatcgcgc cctgcgcgcg cacctgtgcg
    20821 tagtcgcgca cgcgccgcag cagccggttc gccacgcgcg gcgtgccccg cgcgcgtttc
    20881 gcgatctcca gcgcaccgtc gggcgagatc tcgaccgcca gcagcgcggc ggagcggttc
    20941 acgatcttct gcagctccgc gtcgctatag aactcgagcc gcgcgacgat gccgaagcgg
    21001 tcgcgcaaag ggttcgtcag catgccggcc cgcgtcgtcg cgccgaccag cgtgaagggc
    21061 ggcaggtcga gcttcaccga gcgcgccgcg gggccctcgc cgatcatgag gtcgatctgg
    21121 tagtcctcga gggcgggata caggatctcc tcgaccacgg gcgacaaccg gtggatctcg
    21181 tcgatgaaca ggacgtcgtt cggctcgagg ttggtgagga tggccgcgag gtcgcccggc
    21241 cgctcgagca ccggtcccga cgtgtgccgg agattgacgc ccatctcgcg cgcgacgatg
    21301 tgcgcgagcg tcgtctttcc gagtccaggc ggcccgaaca ggagcacgtg gtcgagcgct
    21361 tccttgcggt tgcgcgccgc ctcgacgaag atcgcgagct gcccgcggat cttctcctga
    21421 ccgacgtact cggcgagcga cttggggcgc agcgcgcgct cgaacgcgtc ctcctgcgcg
    21481 gcgcccgcgc ccgagatcag gcggtcggtc tcgatcgaca ttgccctatc ttaccgccgg
    21541 cggttcacgc cttcgagagc gacttgagcg cctggcggat gctctcggag agcgacagcc
    21601 cctcgggcag ctgcttcacg gcttgcgcgg cctccttgtc gctgtagccg agcgcgagga
    21661 gcgcgttcac cacgtcgc
//
LOCUS       DATDMW010000213         6606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_562047, whole genome shotgun sequence.
ACCESSION   DATDMW010000213 DATDMW010000000
VERSION     DATDMW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562047"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VI319_09350"
     CDS             complement(<1..838)
                     /locus_tag="VI319_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY6864093.1"
                     /translation="MEKLLIVGCGDIARRALPELLRRYRVSALVRTRDPSLVSAGAGL
                     VEGDLDRAETLAALPAGALRIAHFAPPSEGGTHDLRTRALLAALETAARRGEMLPQRF
                     VYLSTSGVYGDCGGARVDETRAPFPQTERAARRLDAEETLREWGGRLGVEIVVLRVPG
                     IYAADRLPIERLRRGVPTLRAEDDVYTNHIHADDLAAAVIAALDGNAPPGVYNVSDDS
                     EIRVGDWFDLVAGRCGLPRPPRIARIDAAGLIPAAQLSFMSESRRLVNRRMKDGLGVR
                     LRY"
     gene            863..1888
                     /locus_tag="VI319_09355"
     CDS             863..1888
                     /locus_tag="VI319_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEY6864094.1"
                     /translation="MTHQVTIRNTGHQFPAQEGESILQAALNAGLVLPYGCRDGACGT
                     CKGKLVEGTLDYGRYSEKVLTPAEREQGHALFCQAKPLGDVVIEAREVRRAGDIPVKK
                     LPARVQRMQRATDDVMILYLKLPANERLQFLPGQYVDLLLKDGTRRSFSMGNAPHDDE
                     FVQLHVRHVAGGSFTDHVFATMKERDILRFEGPLGTFFLREESDKPVVFVASGTGFAP
                     IKAIIEAALQKGVTRPMTLYWGGRRPRDLYLDELPKQWVAAHPGFEYVPVISDALPED
                     RWTGRTGFVHRAVMQDFPDLSGHQVYACGVPVMVDSARRDFVAQCRLPENEFYADSFT
                     TAADKAA"
     gene            complement(1889..3964)
                     /locus_tag="VI319_09360"
     CDS             complement(1889..3964)
                     /locus_tag="VI319_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY6864095.1"
                     /translation="MSAAQYAVDGAVAVIRLDNPPMNTLAHALREALVQQLARAAADG
                     AVKAVVLAGAGRAFCAGAEIREFNTPKSVASPNLRELIAAIEALDKPVVAAIHGVAMG
                     GGLELALGCHYRVAAPGAQLALPEVKLGLIPGAGGTQRLPRALGVGRALGMIVSGEPV
                     KSEQVAGTGLVDEIIAGELVPGAVGFAERIAARRPLRRLRDLSVTPDAVAFAEARKKV
                     AKEQRGYPAPARAVEAIEGATTLAFDDGVRNERLIFDELVLTPESKALRHAFFGERAV
                     AKVPDVPEDTPTIDVRRVAVVGAGTMGGGIAMAFANAGFPVTVLETSREALERGLATV
                     RRNYAGAVARGRLAQGDMDARLARIGSTLSYDDLRDADLVIEAVFEDMRVKKDVFAAL
                     DRAMKPGAILATNTSTLDVDEIAAATSRPESVIGLHFFSPANVMRLLEIVRAKRTSKP
                     VLATALKLARTIRKLGVVSGVCDGFIGNRMVEQYLRQAGFLLDEGALPHQVDRALQEW
                     GMAMGPFAMSDLAGNDIGWAIRKRRRVERPDFTYSAIPDRICELGRFGQKTGAGFYRY
                     EPGGRAPIPDPEIDKLIVDHSRAIGVERRTITDAEIVERCIYALVNEGARILEEGIAM
                     RAVDIDMVYIAGYGFPPYRGGPMFYADTVGLDKIVAAIESYARGYRGESWEVAPRLAR
                     LAREGRTFN"
     gene            complement(3987..4697)
                     /locus_tag="VI319_09365"
     CDS             complement(3987..4697)
                     /locus_tag="VI319_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864096.1"
                     /translation="MLEVRGLRVAYGGIAAVKGIDLEVGRGELVCLIGANGAGKTTTL
                     KAIAGALAPAAGSVLLEGREVAGVPAHELVRRGLALVPEGRGVFARLTVAENLRMGAY
                     CRDDAGIEPDLERVYGLFPRLRERRRQIAGTLSGGEQQMLAIGRALMSRPKLLLLDEP
                     SMGLAPIAAKAVFATIRGIAADGVTVLLVEQNALAALELARRAYVMESGSIALAGEAR
                     ALLGDPKVREAYLGETTS"
     gene            complement(4690..5457)
                     /locus_tag="VI319_09370"
     CDS             complement(4690..5457)
                     /locus_tag="VI319_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864097.1"
                     /translation="MSALLAASDVSKRFGGVQALSGVSLAIGRGEIYGLIGPNGAGKT
                     TFFNVLTGLYRPDDGELALEGAPLAAGAPHEVARRGIARTFQNIRLFSNMSAWENVMV
                     GRHVRTRAGVLGAVLRDRRTRAEERAIHEKALALLDYVGLAARRDDLARNLSYGDQRR
                     LEIARALATEPRLLALDEPAAGMNATERESLRRLLESIRSGGVTLLLIEHDVKLVMGL
                     CDRVAVLDYGRKIAEGRPAEVQRDPAVIEAYLGGAHA"
     gene            complement(5454..6512)
                     /locus_tag="VI319_09375"
     CDS             complement(5454..6512)
                     /locus_tag="VI319_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864098.1"
                     /translation="MVTAPRRTLAWTGLALVALVLAVLPLALASAFGLAWVRVVNLAL
                     LYVMLALGLNIVVGFAGLLDLGYIAFYAVGAYCYALLSSPQFGLHLPFWITLPLGFLL
                     AAFFGVLLGAPTLKLRGDYLAIVTLGFGEIIRIFLNNLNAPVNITNGPQGVNLIDPLR
                     LGSFSLASRGSFLGLDFGGPQKYYYALLAVTLGIVALNLRLQDSRIGRAWEAIREDET
                     AAKAMGINTRNIKLLAFAMGASFGGLAGGLFASIQGFISPESFGLLESVTVLAMVVLG
                     GMGNIPGVILGAVLLTAVPEVIRYTVVPAQQAMFGRVLVDAEALRMLLFGLALILVMV
                     LRPAGLWPATVRKREFAR"
     gene            complement(6514..>6606)
                     /locus_tag="VI319_09380"
     CDS             complement(6514..>6606)
                     /locus_tag="VI319_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6864099.1"
                     /translation="YQDVFAFMVLVVVLTIRPSGLLGERVSERA"
BASE COUNT          993 a         2407 c         2303 g          903 t
ORIGIN      
        1 gatagcgcag ccgcacgccg agcccgtcct tcatgcgccg gttcaccagg cgccgcgact
       61 cgctcatgaa cgacagctgc gcggcgggaa tcaggcccgc cgcatcaatg cgggcgatcc
      121 tcggcgggcg cggcaggccg cagcgcccgg cgacgaggtc gaaccagtcg cccacgcgga
      181 tctcgctgtc gtcgctcacg ttgtagacgc cgggcggtgc attgccgtcg agcgccgcga
      241 tgaccgccgc cgcgagatcg tcggcgtgga tgtggttggt gtacacatcg tcctcggcgc
      301 gcagggtcgg cactccgcgg cgaagccgct cgatcggcag ccggtcggcg gcgtagatgc
      361 cgggcacgcg caggacgacg atctcgacgc cgaggcgtcc gccccactcg cgcagcgtct
      421 cttcggcgtc gaggcgccgc gcggcgcgtt ccgtctgcgg gaacggcgcg cgcgtctcgt
      481 ccacccgcgc accaccgcag tcgccgtaga cgccgctcgt gctcagatac acgaaacgct
      541 gtggtaacat ttcgccgcgt cgcgctgcag tctcgagcgc ggcgagcaaa gcccgggtgc
      601 gaaggtcgtg cgtgccaccc tcggagggcg gcgcgaagtg cgcaatgcgc aaggcgcctg
      661 cggggagcgc ggcgagcgtc tcggcgcggt cgaggtcgcc ttcgacgagc ccggcgccgg
      721 ccgacacgag cgatggatcg cgcgtgcgca ccagcgccga cacgcggtag cgccgcagca
      781 gctcgggcag cgcgcgccgg gcgatgtccc cgcagccgac gatgagcaac ttctccatgg
      841 gcgaatttta cccccgcctc cgatgacgca tcaggtcacg atacgcaata ccgggcatca
      901 attccccgcg caggagggcg aaagcatcct ccaggcggcg ctgaacgccg gcctcgtcct
      961 gccttacggc tgccgcgacg gtgcctgcgg cacctgcaag gggaagctgg tcgaaggcac
     1021 gctcgattac gggcgctatt ccgagaaggt cctcaccccc gccgagcgcg agcagggcca
     1081 tgctctcttc tgccaggcga agccgctcgg cgatgtcgtc atcgaggcgc gcgaggtgcg
     1141 ccgcgccggg gacatcccgg tcaagaagct ccccgcgcgg gtccagagga tgcagcgcgc
     1201 gaccgacgac gtgatgatcc tgtacctgaa gctgccggcg aacgagcgcc tgcagttcct
     1261 gcccgggcag tacgtcgacc tgctgctcaa ggacggcacg cggcgcagct tctcgatggg
     1321 caacgcgccg cacgacgacg agttcgtgca gctgcacgtg cgccacgtcg ccggcggatc
     1381 gttcaccgac cacgtgttcg ccaccatgaa ggagcgcgac atcctgcgct tcgagggacc
     1441 gctcgggacg ttcttcctgc gcgaggaatc ggacaagccg gtcgtcttcg tcgcctcggg
     1501 caccggcttc gcgccgatca aggcgatcat cgaggccgcg ctgcagaagg gcgtgacgcg
     1561 accgatgacg ctgtactggg gcggacggcg gcctcgggat ctctacctgg acgagttgcc
     1621 gaagcagtgg gtcgccgcgc acccgggctt cgagtacgtg ccggtgatct cggatgcgct
     1681 gcccgaggac cgctggaccg gccgcaccgg cttcgtgcac cgcgcggtga tgcaggactt
     1741 ccccgatctc tcgggccacc aggtgtacgc gtgcggcgtc cccgtcatgg tcgattccgc
     1801 gcggcgcgac ttcgtcgcgc agtgccgcct gcccgagaac gagttctacg ccgattcctt
     1861 caccacggcc gccgacaagg ccgcctgatc agttgaaggt cctgccctcg cgtgcgagcc
     1921 gcgcgagccg cggcgcgact tcccacgact cgccgcggta gcccctcgcg tagctttcga
     1981 tcgccgcgac gattttgtcg agccccaccg tatcggcgta gaacatgggt ccaccacggt
     2041 aaggcgggaa gccgtagccc gcgatgtaga ccatgtcgat gtccacggcg cgcatcgcga
     2101 tgccttcctc gaggatgcgc gcgccctcgt tcaccagcgc gtagatgcag cgctcgacga
     2161 tctcggcgtc ggtgatcgtg cgtcgctcga cgccgattgc gcgcgagtga tcgacgatca
     2221 gcttgtcgat ctcggggtcg ggaatcggcg cgcggccgcc cggctcgtag cggtagaagc
     2281 ccgcccccgt cttctggccg aagcgtccca gctcgcagat ccggtccggg atcgcggagt
     2341 aggtgaagtc gggccgctcg acgcgccgcc gcttgcggat cgcccagccg atgtcgttcc
     2401 cggcgaggtc ggacatcgcg aacgggccca tcgccatgcc ccactcctgc agcgcgcggt
     2461 cgacctggtg cggcagcgcg ccttcgtcga gcaggaaccc ggcctggcgc aggtactgct
     2521 cgaccattcg gttgccgatg aagccgtcgc acacgcccga cacgacgccg agcttgcgga
     2581 tcgtcctggc gagcttcagc gccgtcgcga gcaccggctt cgacgtgcgc ttcgcccgca
     2641 cgatctcgag caggcgcatg acgttcgccg gcgagaagaa gtggagcccg atcaccgact
     2701 cgggacgcga ggtcgcggcc gcgatctcgt cgacgtcgag cgtcgacgta ttggtcgcca
     2761 ggatcgcgcc gggcttcatc gcccggtcga gcgccgcgaa cacgtccttc ttcacgcgca
     2821 tgtcctcgaa caccgcctcg atcacgaggt cggcgtcgcg gaggtcgtcg tacgagaggg
     2881 tcgagccgat gcgcgcgagc cgcgcgtcca tgtcgccctg cgcgaggcgg ccgcgcgcga
     2941 ccgcgcccgc ataattcctg cgcaccgtcg ccagcccgcg ctcgagcgcc tcgcgactcg
     3001 tctcgagcac cgtcaccgga aagcccgcgt tggcgaacgc catcgcgatg ccgccgccca
     3061 tggtcccggc gccgacgacg gcgacgcgcc tgacgtcgat cgtgggcgtg tcctcgggca
     3121 cgtcggggac cttggccacg gcgcgctcgc cgaaaaacgc gtggcgcagc gccttcgact
     3181 ccggggtgag gacgagctcg tcgaagatga ggcgctcgtt ccggacgcca tcgtcgaacg
     3241 cgagcgttgt cgcgccttcg atcgcctcga ccgcgcgtgc gggcgccggg tagccgcgct
     3301 gctctttcgc gaccttcttc cgcgcctcgg cgaacgcgac cgcgtccggc gtcacgctca
     3361 ggtcgcgaag gcgccggagc ggccgccgcg cagcgatgcg ctcggcgaac ccgaccgctc
     3421 ccggcaccag ctcgcccgcg atgatctcgt cgacgagccc cgtgcccgcg acctgctccg
     3481 atttcaccgg ctcgccggag acgatcatcc ccagcgcgcg cccgacgccg agcgcgcgcg
     3541 gcaggcgctg cgtcccgccc gcgcccggga tcagcccgag cttcacctcg ggcagcgcga
     3601 gctgcgcgcc cggcgcggcg acgcgatagt ggcagccgag cgcgagctcg aggccgccgc
     3661 ccatggcgac gccgtggatc gcggcgacca ccggcttgtc gagcgcctcg atcgcggcga
     3721 tcagctcgcg caggttcggc gaggcgaccg acttcggcgt gttgaactcg cggatctcgg
     3781 cgccggcgca gaaggcgcgg ccggcgcccg cgagcacgac cgccttcacc gcgccgtcgg
     3841 ccgccgcgcg cgcgagctgc tgcacgagcg cctcgcgcag cgcgtgcgcg agcgtgttca
     3901 tcggcgggtt gtcgagccgg atgacggcga ccgcgccgtc gactgcgtat tgcgctgcgc
     3961 tcatgcgtcc tcccgggcga acgcgatcac gaggttgtct cgccgaggta cgcctcgcgc
     4021 accttcgggt cgccgagcag cgcccgcgcc tcgccggcga gggcgatgct gccgctctcc
     4081 atcacgtagg cgcggcgcgc gagctcgagc gccgcgagcg cgttctgctc gacgagcagg
     4141 acggtgacgc cgtcggcggc gataccgcgt atggtcgcga acaccgcctt cgcggcgatc
     4201 ggggcgagcc ccatgctcgg ctcgtcgagc agcagcagct tcggacggct catgagcgcg
     4261 cgtccgatcg cgagcatctg ctgctcgccg cccgagagcg ttcccgcgat ctggcggcgc
     4321 cgctcgcgca gccgcggaaa cagaccgtac acgcgctcga ggtcgggctc gatgccggcg
     4381 tcgtcgcggc aataggcgcc catccggaga ttctcggcga ccgtgagccg ggcgaacacg
     4441 ccgcgtcctt cgggcacgag cgccaacccc cggcgcacca gctcgtgcgc cggaacgccg
     4501 gcgacctcgc ggccctcgag cagcacgctg ccggcggcgg gcgcgagcgc gcccgcgatc
     4561 gccttcagcg tcgtcgtctt gcccgcgccg ttggcgccga tgaggcagac cagctcgccg
     4621 cgcccgacct cgagatcgat gcccttgacc gcggcgatgc cgccgtaggc cacgcgcaag
     4681 ccgcgcacct caagcatgcg cgcccccgag gtaggcttcg atcacggccg ggtcgcgctg
     4741 gacctccgcg ggtcggccct cggcgatttt gcggccgtaa tcgaggaccg cgacgcggtc
     4801 gcacaggccc atgaccagct tcacgtcgtg ctcgatcagc agcagcgtca ccccgcccga
     4861 gcggatcgac tccagcagcc gacgcagcga ctcgcgctcg gtcgcgttca tcccggccgc
     4921 gggctcgtcg agcgcgagca gccgcggctc cgtcgcgagc gcgcgcgcaa tctcgagccg
     4981 gcgctggtcg ccgtaggaga ggttgcgcgc gaggtcgtcg cgccgcgcgg cgagcccgac
     5041 gtagtcgagc agcgccagcg ccttctcgtg gatggcgcgc tcctcggcgc gcgtgcggcg
     5101 gtcgcgcagc accgcgccga gcacgcccgc gcgcgtgcgc acgtgccggc ccaccatgac
     5161 gttctcccag gcgctcatgt tggagaacag ccggatgttc tggaaagtgc gcgcgatccc
     5221 gcgccgggcg acctcgtgcg gcgctcccgc cgcgagcggc gcgccctcga gcgcgagctc
     5281 accgtcgtcg ggacggtaca ggccggtgag cacgttgaag aacgtcgtct tcccggcgcc
     5341 gttcgggccg atcagcccgt agatctcgcc gcgcccgatc gcgagcgaca cgcccgagag
     5401 cgcctgcacg ccgccgaagc gcttgctgac gtcgctcgcc gcgagcagcg cgctcaccgc
     5461 gcgaattccc gcttgcgcac cgtcgcgggc cacagccccg ccggccgcag caccatcacc
     5521 aggatgagcg cgaggccgaa gaggagcatc cgcagcgcct cggcgtcgac cagcacccgg
     5581 ccgaacatcg cctgctgcgc gggcacgacg gtgtagcgga tgacctcggg caccgcggtg
     5641 agcagcactg cgccgaggat cacgccgggg atattgccca tgccgccgag caccaccatc
     5701 gcgagcaccg tgaccgactc gagcagtccg aagctctcgg gactgatgaa gccctggatc
     5761 gacgcgaaca gcccgcccgc gaggcccccg aacgaggccc ccatcgcgaa ggcgagcagc
     5821 ttgatgttgc gcgtgttgat gcccatcgcc ttggccgcgg tctcgtcctc gcggatcgcc
     5881 tcccaggcgc ggccgatgcg cgagtcctgc aggcgcaggt tcagcgcgac gatgccgagc
     5941 gtgacggcga gcagcgcgta gtagtacttc tgcggcccgc cgaagtcgag gccgaggaag
     6001 ctgccgcgcg aagcgagcga gaagcttccg agccggagcg gatcgatcag gttcacgccc
     6061 tgaggtccgt tggtgatgtt caccggcgcg ttcagattgt tgaggaagat gcggatgatc
     6121 tcgccgaagc cgagcgtcac gatcgcgagg tagtcgccgc gcagcttgag cgtgggcgcg
     6181 ccgagcagca cgccgaagaa cgcggcgagc aggaagccca gcggcagcgt gatccagaac
     6241 ggcaggtgca gcccgaactg cggcgacgag agcagcgcgt agcagtacgc gcccaccgcg
     6301 tagaacgcga tgtagccgag atcgagcagc ccggcgaagc cgaccacgat gttgagcccg
     6361 agcgcgagca tcacgtagag cagcgcgagg tttacgacgc gcacccacgc gaggccgaac
     6421 gccgacgcga gcgcgagcgg cagcacggcg agcacgaggg cgacgagcgc gaggccggtc
     6481 cacgcgagcg tccggcgtgg tgcggtcacc atgtcaggcg cgctccgaga cccgctcgcc
     6541 cagcagaccc gacggccgga tcgtcagcac cacgacgagc accatgaacg cgaacacgtc
     6601 ctggta
//
LOCUS       DATDMW010000214         3944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1061514, whole genome shotgun sequence.
ACCESSION   DATDMW010000214 DATDMW010000000
VERSION     DATDMW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061514"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1309)
                     /gene="rodA"
                     /locus_tag="VI319_09385"
     CDS             complement(116..1309)
                     /gene="rodA"
                     /locus_tag="VI319_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY6864100.1"
                     /translation="MIEAVNTRARGLRPQRAEAVGAASALRRLDWWLLGATAALLAFG
                     LWAIGGITAHDVTGNANYYVVRQGIYAAVGCVGFVALLFVEPQIFRRYKRWIYGCTTG
                     VMLLVLVGGTVSRHSKRWLDIGPFRFQPSEFGKLLFILFLAGFLADRARRLGESRTVL
                     EAIGLACVPILLVFVQPDVGTAMVYMAALAAVLFIAGVRWVHLAALGVGTLIAILAVL
                     WFLPAAGVHVLKPYQEQRLTGFTHPNTDPAGATYNVAQSINSVGAGGVRGRGEAGATQ
                     TNLNFLPEHATDFAFASLAEQRGFIGVSVLLLLYLLVVWRGLKVIAIARDAFSAIVAG
                     GIVFAFLFQVFINIGMNIGVAPVTGIPLPFVSVGGSAMIANLLALGVLQAIHIRGASR
                     PKAYR"
     gene            complement(1306..3354)
                     /gene="mrdA"
                     /locus_tag="VI319_09390"
     CDS             complement(1306..3354)
                     /gene="mrdA"
                     /locus_tag="VI319_09390"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY6864101.1"
                     /translation="MASSGTRLPRTGSDRFLPPDPRVEGPYRFTPQLALRIGILGAVA
                     LAVFGVLFFRLWALQVLSGPQYLRAAMDNQLRSVRVEAPRGQILDAKGRPLVTNVPGT
                     AVEIHPGDLPDTWAARLDELRRLSKVVHVPVLQMLRGIAARKDDPVTPVVVREDVKRT
                     APINYLYEHQDEFPGVTVADTFLRGYPQGDLAAQVLGYVNEISPDQLKRLKRKGYLAG
                     DKIGGAGVEATYDSYLRGRAGLAQVRVDSLGRPRSQLETKRPAAQGESIKLTIDTNLQ
                     RAAEDAIRYGINKALLNHQWQADGGAIVALDPRNGAIRALASYPTYYPKVYTGRVTTK
                     KLAAQGLTPTTAERRNYPALNRAISGVYPAGSTFKPVTALAALETHILPSPSTSLECT
                     GDFSVPNQYGGPPQVFKNVDPFVFESMTLPTALAVSCDTWFYRLGYDFYNAPPSMGHP
                     LQAWAARFGFGRKSGVDVGPESGGLLPTPEWRKQAFTPKTDPRQWQIDRIWKPGDSIQ
                     LAIGQKDLLVTPLQMARFYALIANGGKLVRPHVVSDVEEGGGGPRSPARVVHTFTPPA
                     PQSVGLDPTYLAAVQDGLWQATHSQNGTATGVFGTFPIQIAGKTGTAQKNPNTDQLDQ
                     SWWCGYGPADGYESPQLVVCALIENGGFGADAAAPAALQVFKEYFKNRLTSTGVGARP
                     "
     gene            complement(3347..>3944)
                     /gene="mreD"
                     /locus_tag="VI319_09395"
     CDS             complement(3347..>3944)
                     /gene="mreD"
                     /locus_tag="VI319_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY6864102.1"
                     /translation="LAERPLRLAALGDRPRAQEAPVNVNLLEPAKAALLIFVASILQV
                     TIFSEIDILGGYPDVVLLTLVAIALLRGSIYGAACGFFAGLLVDTATLSTLGVTSLLL
                     TITGYWIGRYGETTGRDRLHAPFVSVAVATVLVQIGALILHYMLGDDVSARTVLLDAL
                     PAKVGLNLLLTLPVYALTRRLLAIASTPETATEVRLLG"
BASE COUNT          600 a         1357 c         1449 g          538 t
ORIGIN      
        1 tccggcatat cttcggcaag ctcggcctgc gccccgccga cggcgatcat cgccgcgtgc
       61 tggctgtgct cgcgtatctc cgctctacgg agtaggccct tcgacgaagc cggcgctagc
      121 ggtaggcctt cgggcgcgac gcgccgcgga tgtggatcgc ctgcagtacg cccagcgcga
      181 gcaggttcgc gatcatcgcc gagccgccga cgctcacgaa cgggagcggg atgcccgtca
      241 ccggcgcgac gccgatgttc atgccgatgt tgatgaagac ctggaacagg aacgcgaaca
      301 cgatcccgcc cgcgacgatc gccgagaagg cgtcccgcgc gatcgcgatc accttcaggc
      361 cgcgccacac gacgagcagg tagagcagca gcagcaccga gacgccgatg aagccgcgct
      421 gctcggccag cgacgcgaac gcgaagtcgg tcgcgtgctc gggcaggaag ttcaggttcg
      481 tctgggttgc gcccgcctcg ccgcggccgc gcacgccgcc ggcgccgacc gagttgatcg
      541 actgcgccac gttgtacgtc gcccccgccg gatccgtgtt cgggtgcgtg aacccggtga
      601 ggcgctgctc ctggtagggc ttgagcacgt gcacccccgc cgcgggcagg aaccagagca
      661 ccgcgaggat cgcgatcagc gtccccacgc cgagcgcggc caggtgcacc cagcgcacgc
      721 cggcgatgaa gagcacggcc gcgagcgccg ccatgtacac catcgcggtg ccgacgtccg
      781 gctggacgaa gacgagcagg atcggaacgc acgcgagccc gatcgcctcg agcacggtcc
      841 ggctctcccc cagccgccgc gcgcggtcgg cgaggaagcc ggcgaggaag aggatgaaca
      901 gcagcttccc gaactcggac ggctggaagc ggaacggccc gatgtcgagc cagcgcttcg
      961 agtggcgcga gacggtgccg ccgacgagca cgagcagcat cacgcccgtg gtacagccgt
     1021 agatccagcg cttgtagcgc cggaagatct gcggctcgac gaacagcagc gcgacgaagc
     1081 cgacgcaccc gaccgccgcg tagatgccct ggcggacgac gtagtagttc gcgtttcccg
     1141 tgacgtcgtg ggcggtgatg ccgccgatcg cccagaggcc gaacgcgagc agggcggcgg
     1201 tggcgcccag gagccaccag tccagccggc gcagcgcgga ggccgcgccg accgcctcgg
     1261 cgcgttgcgg ccgcagccct cgcgcgcggg tgttgacggc ctcgatcacg gccgcgcccc
     1321 cacgccggtc gaggtcaggc ggttcttgaa gtactccttg aagacctgca gcgcggcggg
     1381 agcggcggcg tcggcgccga agccgccgtt ctcgatcagc gcgcatacca cgagctgcgg
     1441 cgactcgtac ccgtcggcgg gcccgtagcc gcaccaccac gactggtcga gctggtcggt
     1501 gttcgggttc ttctgcgcgg tgccggtctt gcccgcgatc tggatcggga aggtcccgaa
     1561 gacgcctgtc gcggtgccgt tctgcgagtg cgtcgcctgc cagaggccgt cctgcaccgc
     1621 cgcgaggtag gtcgggtcga ggccgaccga ctgcggcgcg ggcggcgtga aggtgtggac
     1681 gacgcgcgcg ggcgagcgcg ggccgccgcc gccctcctcg acgtcggaga cgacgtgggg
     1741 gcgcacgagc ttgccgccgt tggcgatcag cgcgtagaag cgcgccatct gcagcggcgt
     1801 cacgagcagg tccttctgcc cgatcgcgag ctgaatcgag tcgccgggct tccagatgcg
     1861 gtcgatctgc cactgccggg ggtcggtctt cggcgtgaac gcctgcttgc gccactcggg
     1921 cgtcggcagc agcccgcccg actcgggccc gacgtccacg ccgctcttgc ggccgaagcc
     1981 gaaccgcgcc gcccaggcct gcagggggtg gcccatgctc ggaggcgcgt tgtagaagtc
     2041 gtagccgagc cggtagaacc acgtgtcgca cgagaccgcc agcgccgtcg gcagcgtcat
     2101 cgactcgaag acgaacgggt cgacgttctt gaacacctgc gggggcccgc cgtactggtt
     2161 cgggacggag aagtcgcccg tgcactcgag cgaggtcgag ggcgacggga ggatgtgcgt
     2221 ctcgagggcc gcgagcgcgg tcacgggctt gaacgtcgag cccgcgggat acacgccgct
     2281 gatcgcgcgg ttgagcgccg ggtagttgcg gcgctcggcc gtcgtcggcg tcagcccctg
     2341 cgcggcgagc ttcttggtcg tgacacgccc cgtgtagacc ttcgggtagt acgtcgggta
     2401 cgaggcgagc gcgcggatgg cgccgttgcg ggggtcgagc gcgacgatcg cgccgccgtc
     2461 ggcctgccac tggtggttga ggagcgcctt gttgattccg tagcggatcg cgtcctcggc
     2521 cgcgcgctgg aggttggtgt cgatcgtcag cttgatcgac tcgccctggg cggccgggcg
     2581 cttcgtctcg agctggctcc gcggccggcc gagggagtcc acgcgcacct gggcgaggcc
     2641 tgcgcggccg cggagatacg agtcgtaggt cgcctccacc ccggccccgc cgatcttgtc
     2701 gccggccagg tagcccttcc gcttcaggcg cttcagctgg tcgggcgaga tctcgttgac
     2761 gtagccgagc acctgcgcag cgaggtcgcc ctgcgggtag ccgcgcagga aggtgtccgc
     2821 gacggtcacg cccgggaact cgtcctggtg ctcgtagagg tagttgatcg gcgcggtccg
     2881 cttcacgtcc tcgcgcacga cgaccggcgt caccgggtcg tccttgcgcg cggcgatccc
     2941 gcgcagcatc tgcagcaccg gcacgtgcac gaccttcgac aggcgcctga gctcgtccag
     3001 ccgcgccgcc cacgtgtccg ggaggtcgcc ggggtggatc tcgaccgcgg tgcccgggac
     3061 gttcgtgacc agcggccggc ccttggcgtc gaggatctgt ccgcgcgggg cctccacgcg
     3121 caccgagcgc agctggttgt ccatcgccgc gcgcaggtac tgcgggcccg aaagcacctg
     3181 tagcgcccag aggcggaaga agagcacgcc gaacaccgcc agcgcgaccg ccccgaggat
     3241 cccaatgcgc agcgcgagct gcggcgtgaa gcggtacggg ccctcgacgc gcgggtcggg
     3301 cggcaggaag cggtccgagc ccgtgcgcgg caggcgcgtg ccgctgctag ccaaggagcc
     3361 gcacctctgt cgcggtctcg ggcgtcgagg cgatcgccag caggcggcgc gtgagcgcgt
     3421 agaccggcag cgtcagcagc aggttcaggc cgaccttcgc cgggagcgcg tcgagcagca
     3481 ccgtccgcgc ggagacgtcg tcgcccagca tgtagtggag gatcagcgcc ccgatctgga
     3541 cgagcaccgt ggccaccgcg accgagacga acggcgcgtg cagccggtcg cggccggtcg
     3601 tctcgccgta gcggccgatc cagtagccgg tgatcgtcag cagcagcgag gtgacgccga
     3661 gcgtcgagag cgtcgcggtg tccacgagca gccccgcgaa gaacccgcac gcggcgccgt
     3721 agatcgagcc ccgcagcagc gcgatcgcga ccagcgtcag cagcaccacg tcggggtagc
     3781 cgccgaggat gtcgatctcg gaaaagatcg tcacctgcag gatcgaggcg acgaagatga
     3841 gcagcgcagc cttggcgggc tcgagcaggt tgacgttcac gggcgcttct tgggcacgag
     3901 gacgatcacc gagcgcagcg agtcgaagtg gacgctcggc gaga
//
LOCUS       DATDMW010000215         5818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_314051, whole genome shotgun sequence.
ACCESSION   DATDMW010000215 DATDMW010000000
VERSION     DATDMW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314051"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VI319_09400"
     CDS             <1..948
                     /locus_tag="VI319_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6864103.1"
                     /translation="RDMLGVLEEWGDLYDEPFGDQSGVPTYLVSKMARRHVKVALSAD
                     GGDELFSGYARYALTLARQRRLVRVPRAARVALSHALEILAPAQHVVERLPVSGVRHA
                     TRRTVVDRVEKLRHVLPDADPGTIYDLSMSFWLPGETSRLLGETVHPRPALNGYPRKF
                     PDHMSCCDIRHYLPDDILTKVDRTTMAVGLEGREPLLDHRLVEFALRLPYELRQGSLG
                     TKHLLRRVLYRYVPKSVLERPKQGFSMPMSRWLRGDLAPLIDNYLDPQRVRAAGVLDP
                     AVTERAVRNFREGGPARDRIDVQKVWLLVAFEMWREKWA"
     gene            956..2113
                     /locus_tag="VI319_09405"
     CDS             956..2113
                     /locus_tag="VI319_09405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6864104.1"
                     /translation="MKILYVISELTLGGAQKQLVELAKQLAARGHEIRIYTLNDDVPR
                     RAELAGSAVELIVDQKQSRLDLAVLRRLRAQILRWRPDIVHGFLFDGHLYSRLAAAGT
                     GIPVLNSERSHNYRLTPVQRFAHHFTRSLARGVVANTHAGADFARRLFALAPDDVHVV
                     WNGVRVDEIEHGARTALDYKFRFFGDARVKLACLVGAIKPAKDYHLALATAARLVESD
                     PAWRVLFVGDRLAASGRYRANPESDTGGYKAAVLRHYDSLGLEDRIKFAGLRTDVPAI
                     VRQCDVLYVTSEHEGFPNVVLEAMTLGVPVASTEYSDIRRILPCEWQVVSARSAQGLA
                     DAIRRASDERVTVAARQRQWVLANATVEIAAASLERIYEKYARPEICAQLA"
     gene            2130..3293
                     /locus_tag="VI319_09410"
     CDS             2130..3293
                     /locus_tag="VI319_09410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6864105.1"
                     /translation="MSSDAAAGAASSRPPSVCFVAPLAWPILAGIAELKIVGGAEVQQ
                     SILARALAEAGFRISMVCNDFGQAEGVRVHGVTVHKTHRLEQGIPVVRFLHPRLSTTW
                     AALRRANADIYYTRSSSMLAGVVAAFCRRHGRRSVYAAASDVDFIPEKQALRFRRDRW
                     MFEYGVRHADAVIVQNAFQQETCYENYGRRATVIPSAYAPPVGATADESGSVLWVGTI
                     RDYKQPELLLEMARRLPQLRFVMVGGAGGLRQQDIRYFEGVKREAQSLRNVEFAGFVP
                     YAGVEAYFNRARVFVNTSRFEGFPNTFLQAWARGVPTVSFVDTGSRQGLEPIYAVVRD
                     VSAAVQEIGRLMLDDDHWHAASARCRMHYWTHHSMSSVTARYTKALMAIGAAR"
     gene            3290..4852
                     /locus_tag="VI319_09415"
     CDS             3290..4852
                     /locus_tag="VI319_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEY6864106.1"
                     /translation="MKGGARAEAALGPSDGVDPGGTTREKRRPIERQALSLWTANAID
                     YGLQFLLPVVLARTLDVADFGRYRLLWLAVATVMALVPLAMPYSLYYFLPRSDAAAKR
                     LYINQTLIFLAVTGSIAAWFISPWNPWLPQSMRGLSGHAALVPSFVALWAVASLLDLL
                     PTVDERVHWQARATVGLSALRAGALSAAALATGELEAVLAVLLAFVAIKVALLFAYVQ
                     RYHGLGRPVARRAAFADQVRYAAPFGVSSALHGLRGQADGWVAATLFSIHQFAAFSIA
                     GVLGPMLTMCRQSFNYAFLPTMSRLQAGGDIAGMLALNNRANMIVAAVSFPLIAFAFV
                     FAEPLIAAVYTAAYADAVPVMRFSCLANVAFVIELHSILLVFRQGPYAMRVNFLALAL
                     GVPCSLAGALFVGLPGAAAGSVAAIYAERAMTLVRVSSLTGVAVHRLQEWGALGAVLA
                     AAIGSAAVAGEVVARVGGDHWHLAQLAEGAAVIALVYPILLVVAGQRRLLFDLVGLLL
                     REKRPMAKPQPE"
     gene            4857..>5818
                     /locus_tag="VI319_09420"
     CDS             4857..>5818
                     /locus_tag="VI319_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6864107.1"
                     /translation="MNTAYDKTLDQLRSAPRTWLVTGVAGFIGSNLLETLLKLDQKVI
                     GLDNFSTGHRGNLSQVKELVTERQWSSFRFIEGDIRSLDACRRACRPAQIVLHQAALG
                     SVPRSIENPIGAHDSNVTGFLNMMMAAHTAGVKRFVYASSSSVYGDDPALPKVEPQTG
                     RPLSPYAATKHMNEVYADVFARCYGLESVGLRYFNVFGPRQDPNGAYAAVIPKWIASM
                     MRHEPVFINGDGETARDFCYIDNVVQANLLAATAQDPEAVNQVYNVALNDKTTLKGLF
                     QAIRTLLEPRYPQLQGVEPVYRDFRPGDVRYSQADVGKAKERLG"
BASE COUNT          804 a         2094 c         2014 g          906 t
ORIGIN      
        1 cgcgacatgc tcggcgtgct cgaggagtgg ggcgacctct acgacgagcc cttcggcgac
       61 cagtcggggg tcccgaccta cctcgtctcg aagatggcgc gccgccacgt gaaggtggcg
      121 ctgtccgccg acggcggcga cgagctcttc agcggctacg cgcgctacgc gctcacgctg
      181 gcgcgccagc gccggctggt gcgcgtgccc cgcgccgcgc gcgtcgcgct gtcgcacgcg
      241 ctcgagatcc tcgcgccggc gcagcacgtg gtcgagcgcc tgccggtcag cggcgttcgg
      301 cacgcgaccc ggcgcacggt ggtcgaccgt gtcgagaagc tgcgccacgt cctccccgac
      361 gccgatcccg gcacgatcta cgacctgtcg atgtcgttct ggctgccggg cgagacctcg
      421 cggttgctcg gcgagacggt gcacccgcgg ccggcgctga acggctaccc gcgcaagttc
      481 cccgaccaca tgagctgctg cgacatccgg cactacctgc cggacgacat cctgaccaag
      541 gtcgaccgca cgaccatggc ggtcgggctc gaagggcgcg agccgctgct cgatcaccgg
      601 ctggtcgagt tcgcgctgcg ccttccgtac gagctgcgcc aggggtcgct cggcaccaag
      661 cacctgctgc gccgcgtgct ttaccgctac gtgcccaagt cggtcctcga gcgccccaag
      721 cagggcttca gcatgccgat gtctcgctgg ctgcgcggcg atctcgcccc gctgatcgac
      781 aactatctcg atccgcagcg cgtgcgcgcc gcgggcgtgc tcgaccctgc ggtgaccgag
      841 cgcgcggtgc gcaacttccg cgaaggcggc ccggcgcgcg accgcatcga cgtgcagaag
      901 gtctggctgc tggtcgcgtt cgagatgtgg cgcgagaaat gggcctgacg cgccggtgaa
      961 gattctctac gtcatcagcg agctcacgct cggcggcgcg cagaagcagc tggtcgagct
     1021 ggcgaagcag ctcgcggcgc gcggccacga gatcaggatc tacaccctga acgacgacgt
     1081 gccgcgccgc gccgagctcg cgggctcggc cgtggagctg atcgtcgacc agaagcagtc
     1141 gcggctcgac ctcgccgtgc tgcggcggct gcgcgcgcag atcctgcgct ggcgtcccga
     1201 catcgtccac ggcttcctgt tcgacgggca cctgtactcg cggctcgccg cggccgggac
     1261 cggcatcccg gtcctgaact ccgagcgcag ccacaactac cggctgacgc cggtccagcg
     1321 cttcgcgcac catttcaccc gttcgctcgc gcgcggcgtg gtcgcgaaca cgcacgcggg
     1381 cgcggacttc gcgcggcgcc tgttcgcgct ggcgcccgac gacgtccacg tcgtctggaa
     1441 cggcgtgcgc gtggacgaga tcgagcacgg tgcgcgcacg gcgctcgact acaagttccg
     1501 cttcttcggc gatgcccgcg tcaagctcgc gtgcctggtc ggcgcgatca agccggcgaa
     1561 ggactaccac ctcgcgctcg cgaccgcggc gcggctggtc gagagcgacc ccgcgtggcg
     1621 ggtgctgttc gtgggggacc ggctcgctgc gtccggaagg taccgcgcga accccgagtc
     1681 cgacacggga ggctacaagg ccgcggtgct ccggcattac gacagcctgg ggctggagga
     1741 ccggatcaag ttcgccgggc tgcgcaccga cgtccccgcg atcgtgcgcc agtgcgacgt
     1801 gctctacgtg acctcggagc acgagggctt cccgaacgtg gtgctcgagg ccatgacact
     1861 cggcgtcccg gtcgcgagca ccgagtactc cgacatccgg cggatcctgc cctgcgagtg
     1921 gcaggtggtc tccgcgcgct cggcgcaggg gctcgccgac gcgatccggc gcgccagcga
     1981 cgagcgcgtg acggtcgccg cgcgtcagcg ccaatgggtc ctcgccaatg cgaccgtgga
     2041 gatcgcggcc gcctcgctcg aaagaatcta tgagaagtac gcacggcctg aaatctgcgc
     2101 tcagctggcc tgacgccgcc gagccggtat tgagctcgga tgccgccgcc ggcgccgcgt
     2161 cctcgcggcc gccgagcgtg tgcttcgtcg cgccgctcgc ctggcccatc ctcgcgggga
     2221 tcgccgagct gaagatcgtc ggcggcgccg aggtgcagca gagcatcctc gcgcgcgcgc
     2281 tcgccgaggc cgggttccgc atatcgatgg tgtgcaacga cttcgggcag gccgaaggcg
     2341 tgcgcgtgca cggcgtcacc gtccacaaga cgcaccgcct cgagcagggc atcccggtgg
     2401 tgcgcttcct gcacccgcgg ctgtcgacga cgtgggcggc gctgcgccgc gcgaacgccg
     2461 acatctacta cacgcgatcc tcgtcgatgc tcgcgggcgt ggtcgccgcg ttctgccggc
     2521 gccacggacg ccgctccgta tacgccgcgg cctccgacgt cgacttcatc ccggagaagc
     2581 aggcgctgcg cttccgccgc gaccgctgga tgttcgagta cggcgtgcgc catgccgacg
     2641 cggtcatcgt ccagaacgcg ttccagcagg agacctgcta cgagaattac ggccgccgcg
     2701 cgaccgtgat cccgagcgcg tacgcgccgc ccgtcggcgc gaccgcggac gagagcggca
     2761 gcgtgctgtg ggtcggcacg atccgcgact acaagcagcc cgagctgctg ctcgagatgg
     2821 cgcgccggct gccgcagctg cgcttcgtca tggtgggcgg cgcgggcggc ctgcgccagc
     2881 aggacatccg ttacttcgaa ggcgtgaagc gcgaggcgca gtcgctgcgc aacgtcgagt
     2941 tcgccggctt cgtgccttac gcgggcgtcg aggcctattt caaccgcgcc cgcgttttcg
     3001 tcaatacgtc gcgcttcgag ggattcccga acaccttcct gcaggcgtgg gcgcgcggcg
     3061 tgccgaccgt gtccttcgtc gacaccggct cgcgccaggg gctggagccg atctacgcgg
     3121 tggtgcgcga cgtgtccgcg gcggtccagg agatcggccg cctcatgctc gacgacgatc
     3181 actggcacgc cgcgtccgcg aggtgccgca tgcactactg gacgcatcat tccatgagct
     3241 ctgtgacggc gcgctacacc aaggcgctga tggcgatcgg agccgcgcga tgaagggcgg
     3301 cgcccgcgcg gaagccgccc tcggtccctc cgacggcgtc gatccgggcg gcacgacccg
     3361 ggagaagcgc aggccgatcg agcgccaggc gctttcgctc tggaccgcga acgcgatcga
     3421 ctacgggctg cagttcctgc tgccggtcgt actcgcgcgc acgctcgacg tcgccgactt
     3481 cggccgctac cgcctgctgt ggctcgcggt cgcgaccgtc atggcgctcg tgccgctggc
     3541 gatgccgtac agcctgtact acttcctgcc gcgctcggac gcggcggcga agcggctcta
     3601 catcaaccag acgctgatct ttctcgcggt gaccgggtcg atcgccgcct ggttcataag
     3661 cccgtggaac ccgtggctgc cgcagagcat gcgcggcctg agcgggcacg cggcgctggt
     3721 gccgagcttc gtggcgctct gggcggtcgc gtcgctgctc gatctgctgc cgacggtgga
     3781 cgagcgcgtt cactggcagg cgcgcgccac cgtcgggctc tcggccctgc gcgccggggc
     3841 gctctcggcg gcggcgctcg cgaccggcga gctcgaagcc gtgctcgcgg tcctgctcgc
     3901 gttcgtcgcg atcaaggtgg cgctgctgtt cgcctacgtg cagcgctacc acgggctcgg
     3961 gcgcccggtc gcgcgccgcg ccgcgttcgc cgaccaggtg cgctacgccg caccgttcgg
     4021 cgtgtcgagc gcgctgcacg gcctgcgcgg gcaggccgac ggctgggtcg cagccacgct
     4081 cttcagcatc caccagttcg ccgcgttctc gatcgcgggc gttctcgggc cgatgctcac
     4141 catgtgccgc cagtcgttca actacgcctt ccttccgacc atgagccggc tccaggccgg
     4201 aggcgacatc gccgggatgc tcgcgctcaa caaccgcgcg aacatgatcg tcgccgcggt
     4261 gtcgtttccg ctgatcgcct tcgcgttcgt gttcgccgag ccgctgatcg ccgccgtcta
     4321 taccgccgcg tacgcggacg ccgtgccggt gatgcgcttc tcctgcctcg ccaacgtcgc
     4381 cttcgtgatc gagctccaca gcatcctgct ggtcttccgg cagggcccct acgcgatgcg
     4441 cgtgaacttc ctggcgctgg cgctcggcgt gccgtgcagc ctcgcgggcg cgctcttcgt
     4501 cggccttccg ggcgcggccg cgggcagcgt ggcggcgatc tacgccgagc gcgcgatgac
     4561 gctcgtccgc gtctcctcgc tgaccggcgt cgccgtccac aggctccagg aatggggcgc
     4621 gctcggggcg gtgctcgcgg cggcgatcgg ctcggcggcg gtcgccggag aggtggtcgc
     4681 aagggtcggc ggcgaccact ggcacctggc gcagctcgcc gagggcgcgg ccgtcatcgc
     4741 gctcgtgtat cccatcctgc tcgtcgtcgc cggccagcgc cggctgcttt tcgatctcgt
     4801 cgggctgctg ctgcgagaga agcggcccat ggccaaaccg caaccggagt gatcccatga
     4861 ataccgcata cgacaaaacg ctcgaccagc tgcggtccgc gccccgaacc tggctcgtga
     4921 ccggggtcgc gggcttcatc ggctcgaacc tgctcgagac gctgctcaag ctcgaccaga
     4981 aggtgatcgg cctcgacaac ttctcgaccg gccaccgcgg caacctgtcg caggtgaagg
     5041 agctggtgac cgagcgccag tggtcctcgt tccgcttcat cgagggcgat atccgctcgc
     5101 tcgacgcctg ccggcgcgcg tgccgccctg cgcagatcgt gctgcaccag gcggcgctcg
     5161 gcagcgtgcc gcgctcgatc gagaacccga tcggcgcgca cgacagcaac gtgaccggct
     5221 tcctgaacat gatgatggcc gcgcacaccg cgggcgtgaa gcgcttcgtg tacgcgagct
     5281 cgagctccgt gtacggcgac gatccggcgc tgcccaaggt cgagccgcag accgggcggc
     5341 cgctgtctcc gtacgcggcg accaagcaca tgaacgaggt ctacgccgac gtgttcgcgc
     5401 gctgctacgg gctggaatcg gtcgggctgc gctacttcaa cgtcttcggc ccgcgccagg
     5461 acccgaacgg cgcgtacgcg gccgtgatcc ccaagtggat cgcgagcatg atgcgtcacg
     5521 agccggtgtt catcaacggc gacggcgaga cggcgcgcga tttctgctac atcgacaacg
     5581 tcgtgcaggc gaacctgctc gctgcgaccg cgcaggaccc cgaggccgtg aaccaggtgt
     5641 acaacgtcgc gctgaacgac aagaccacgc tgaagggact gttccaggcg atccgcacgc
     5701 tgctcgagcc gcgctatccg cagcttcagg gcgtggagcc ggtctaccgc gacttccgcc
     5761 cgggcgacgt gcgctactcg caggccgacg tcggcaaggc gaaggagcgg ctcgggta
//
LOCUS       DATDMW010000216         8038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1810219, whole genome shotgun sequence.
ACCESSION   DATDMW010000216 DATDMW010000000
VERSION     DATDMW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810219"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VI319_09425"
     CDS             complement(<1..1133)
                     /locus_tag="VI319_09425"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEY6864108.1"
                     /translation="MERVSGGALEKILWRVNRLRCMTPAEVGHRVLRLARAQAERAGF
                     SGVVPAADISAVPKPWIGVPPGVDAEAVLAAAARVAAGRLDVFALSDVDLGDPPRWNR
                     DPKTGVEAPLAFGKLLDYRDARLVGDIKYLWEPNRHLQLVTLAQAWALSGEFRWFAAL
                     RDQLDSWFAACPDGRGPNWASALEAAIRLINWSIAWQLLGGAGSALFRDRAGAEFRAR
                     WLDSVYRHARFVAGNFSLYSSANNHLLGEASGLFIAALAWPHWPRARGWARKATAILE
                     REALLQNAPDGVNREQAVSYQQFTLDFLLLPLLAGRAAGIAFSRAYETRIEAMLEYLA
                     SIMDAGGNVPMIGDADDGYVVRLAPAPGVCPYRSLLAPGAALFG"
     gene            complement(1145..2218)
                     /locus_tag="VI319_09430"
     CDS             complement(1145..2218)
                     /locus_tag="VI319_09430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6864109.1"
                     /translation="MSTARPVVLTVGPQPAARARLAADFELSPFDESGSGLSRVLSPG
                     ARLAATTLWRRAAIVHVRASADERARKREASYLRAAKACGAGTVYELGADAVAHGRAG
                     GMVTPLPRRAARVADVVVVPSEYDADAWRRLAPGQSIAALPSAVDAAAYAGIVRQPRD
                     PGTPLRLLFVGGLVRDQGLYEILQGLRLARVQDVVASLLVAGEGPDETALKRFAASLG
                     LDGAVGFCGAVEGEAKLELLRDADAVVAASHAYCAPGPVLEAMAAGLPAIVTRVGAMP
                     EVVSDGVHGLFVERRAPHSIARAIATLAADREGVVRMGEACRARVASGHSVERQAEGL
                     ASLYTDILAARNLRPASSQGSRG"
     gene            complement(2230..3351)
                     /locus_tag="VI319_09435"
     CDS             complement(2230..3351)
                     /locus_tag="VI319_09435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6864110.1"
                     /translation="MTAARTRLIMLGTAFETRGGISAVVNTYRAHGLFERWPIEYIAT
                     HCDGSGWRKLATAARAVRRLVASLARDPNAIVHIHGASRASFWRKSVFMAIALAARCP
                     VVFHLHGGGFATFYEEECGPVRKRIVRFLLERASRIIVLSAQWRAWLASAMRNPNVVC
                     IANPMPMHPEPAAAGRRNTLLFLGRLERAKGVFDLVDAVAALRDTHPDMRLVLAGDDV
                     DGLAAYAAERGVADRVQLTGWVGPERKRALLDTAAAFVLPSYAEGLPISLLEAMAAGL
                     PSVASEVGGIPDIVADGVNGMLIVPGDVAMLTRVLRKLLDDRMLRARLGAAARETVRL
                     RFDADHVIGELEKIYASLGLARTGKGRVRRTARALREAA"
     gene            complement(3356..4780)
                     /locus_tag="VI319_09440"
     CDS             complement(3356..4780)
                     /locus_tag="VI319_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864111.1"
                     /translation="MSFEDLLHPLLDRYMQAPAWVKASAGRAYAWVPARFRFGGAYYR
                     FRDEVAVRDPGALERLARRKLRSTLEWALQTVPAYRDYAPLLARCDDTAELLARMPLV
                     DKKAIKARPESYLSAAVPQSARLKTVTGGSSRDPLEFYLQKHVSRPKEYAFMHDFRAR
                     TGSDGTEPTLALRGRPVPGADRGRLWTWEPIKRQLIFSSAHLDASHMPRYAEALALHR
                     PQFIEAYPSVLYLLARWLVEHPLPAFTSNVSGVMLYSETVYGFQLKLFRDVFHCPVLA
                     HYGHSERVLMAATMPDDDRYFFWPQYGWLELVGLDGRPITQAGVPGEIVGTSFDNRVM
                     PFVRYRTGDLAILSDRGHARLPGFVACERIEGRLQEFIVDRHERLISVVQIGSAHFPE
                     FGAIDSMQYEQKRPGELVLKFVAGDGFTPEMQRRLARAVGDKAGCDCRVVKVREIGRT
                     ARGKQQLIVQHLDIKRYLGTAGAH"
     gene            complement(4770..6476)
                     /locus_tag="VI319_09445"
     CDS             complement(4770..6476)
                     /locus_tag="VI319_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864112.1"
                     /translation="MAAVALARGPDSVASDHDTAAEGSPARLRIGVLADSLLQPRWAV
                     DGLRKIVDSGVGDVVVIAEAGGPRDAGPFLWRAYCAIDARLFGSSPDPLERTDLAAEL
                     RQARLLRRPPEDSGPASAHAWIAEIRAMDLDVLVALGAETCAALESVARYGVWRYGFG
                     PDASESDSLAAVREVAAGAPVTASGLRARLPGAEDRLIYQSWSRTFPLSLARTRHALL
                     PKTGEFAYRALDELRRSGPAWLQSRAPIREEASLDRASIPGTAGMLWRMSLLGSRIAR
                     RVLQKLACVDQWFLAYRFGGDSRWRGDLRQYVAMMPPKDRFWADPFAVVRDGRYWIFF
                     EELVFSRGKAHIAAVEVSPDGARSQPVRVLERPYHLSYPFLIEQDGELLMIPETACNG
                     TVEVYRCTRFPDQWTLEQTLFENVHFTDSTFHRAGDTWWMFANATVAGAEGADELHLY
                     HADRLAGPWKPHRHNPVKSDVRGTRPAGRLFRRGEDLYRPAQIGVPLYGTGVSINRVL
                     RLTPDSYAEEEVERVAPSYPPRLLGIHTLNRAGELRVIDGFFRRSRLRPDVLESLDTH
                     VF"
     gene            complement(6464..7744)
                     /locus_tag="VI319_09450"
     CDS             complement(6464..7744)
                     /locus_tag="VI319_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY6864113.1"
                     /translation="MGTVAVVGLGYVGLPVAVEFGKRRPTVGYDLSRKKIANLRHGID
                     TTGEVAPEELRQAEKLRVSDDPAILAEADYIIVAVPTPVNAARQPDFSPLESASRTVG
                     EHMKPGAIVVYESTVYPGATEEVCVPILEKHSGMRWRQDFHVGYSPERINPGDKEHSF
                     TRIRKVVSGDDAETLEKVAALYGSVVSAGVFPASSIRVAEAAKVIENTQRDLNIALVN
                     ELAIIFDKLGIDTMEVLKAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAELVGYHPE
                     VILAGRRINDNMGPHIARKTIQQMINAGRNIKGARVTVLGLTFKENCPDIRNSKVVDV
                     IRELAEYGVEISVHDPWADAAEALHEYDVRLRAWDDLAPADALVLAVAHRQFLETPTS
                     TYLQKIVRRGCLIDVKSVLDPDPFRREGLQVWRL"
BASE COUNT         1168 a         2865 c         2924 g         1081 t
ORIGIN      
        1 ccgaagagcg ccgcaccggg cgcgagcagc gagcggtacg gacagacgcc tggcgccggc
       61 gccagcctga cgacgtagcc gtcgtcggcg tcgccgatca tgggcacgtt gccgccggcg
      121 tccatgatcg acgccaggta ctcgagcatg gcctcgatgc gcgtctcgta cgcgcgcgag
      181 aaggcgatcc ccgcggcccg gcccgcgagc agcggcagca gcaggaaatc cagcgtgaac
      241 tgctggtacg agacggcctg ctcgcggttg acgccgtcgg gcgcgttctg cagcagcgcc
      301 tcgcgctcga ggatggccgt cgccttgcgc gcccagccgc gcgcgcgcgg ccagtgcggc
      361 caggcgagcg ccgcgatgaa caggcccgac gcctcgccga gcaggtggtt gttcgccgac
      421 gagtagagcg agaaatttcc cgcgacgaag cgcgcatggc ggtagacgct gtcgagccag
      481 cgcgcgcgga actccgctcc cgcgcgatcg cggaacagcg ccgagcccgc gccgccgagg
      541 agttgccacg cgatcgacca gttgatcagc cggatcgccg cctcgagcgc gctcgcccag
      601 ttcggcccgc ggccgtcggg gcaggccgcg aaccagctgt cgagctggtc gcgcagcgcc
      661 gcgaaccagc ggaactcgcc cgagagcgcc cacgcctgcg cgagcgtcac gagctgcagg
      721 tgccggttcg gctcccacag gtacttgatg tcgccgacga ggcgcgcgtc gcggtagtcg
      781 agcagcttgc cgaaggcgag cggcgcctcg acgccggtct tcgggtcgcg gttccagcgc
      841 ggcggatcgc cgaggtcgac gtcgctcagc gcgaagacgt cgagccggcc ggccgcgacg
      901 cgcgcggcgg cggcgagaac ggcctcggcg tcgacgcccg gcggcacgcc gatccagggc
      961 ttcgggaccg cggagatatc ggcggcggga accacgccag agaatcccgc gcgctcggcc
     1021 tgggcccggg cgaggcgcag cacgcggtgg ccgacctccg cgggcgtcat gcaccggagg
     1081 cggttcacgc gccagaggat cttctcgagc gctcctccgc tcacgcgttc catgcgggga
     1141 gcggtcagcc gcgggagccc tgcgaagacg cgggccgcag gttgcgcgcc gccaggatgt
     1201 cggtgtagag gctggcgagc ccctcggcct ggcgctcgac cgagtgcccg ctcgcgacgc
     1261 gagcgcggca cgcctcgccc atgcgcacga cgccctcgcg gtcggcggcg agcgtcgcga
     1321 tagcgcgcgc gatcgagtgc ggggcgcgcc gctccacgaa cagcccgtgc acgccgtcgc
     1381 tcaccacctc gggcatcgcg cccacgcgcg tgacgatcgc cggcaatcca gcggccatcg
     1441 cttccaggac cggcccgggc gcgcagtagg cgtgcgaggc ggcgacgacc gcgtcggcgt
     1501 cgcgcagcag ctcgagcttc gcctcgcctt cgaccgcgcc gcagaagccg accgcgccgt
     1561 cgagccccag gctggcggcg aagcgcttga gcgccgtttc gtccggccct tcgcccgcga
     1621 cgagcagcga ggccacgacg tcctgcacgc gcgcgagccg cagcccttgc aggatttcgt
     1681 acagcccctg gtcgcgcacc aggccgccca cgaacagcag ccgcagcggc gtgccggggt
     1741 cgcgcggctg gcgcacgatg cccgcgtagg ccgcggcgtc gaccgcgctc gggagcgcgg
     1801 cgatcgactg gccgggcgcg agccggcgcc aggcgtcggc gtcgtattcc gagggcacga
     1861 cgacgacatc ggcgacgcgc gccgcgcgcc gcggcagcgg cgtcaccatg ccgcccgcgc
     1921 gcccgtgcgc gacggcgtcg gcgccgagct cgtacaccgt gcccgcgccg caggccttcg
     1981 cggcgcgcag gtacgaggcc tcgcgcttgc gcgcgcgctc gtccgccgac gcgcggacgt
     2041 ggacgatcgc ggcgcgccgc cacagcgtgg tcgccgcgag ccgcgctccc ggcgagagca
     2101 cgcgcgacag tccgctcccg ctctcgtcga aaggcgagag ctcgaagtcc gccgcgagcc
     2161 gcgcccttgc cgcgggctgc ggtcccaccg tcagtaccac cggtcgtgcc gtactcatcg
     2221 attctccttt caggccgcct cgcgcagagc gcgcgcagtc cgccgcaccc tccccttgcc
     2281 ggtgcgcgcg agccccaggc tcgcgtagat cttctccagc tccccgatga cgtgatcggc
     2341 gtcgaagcgc agccgcacgg tctcgcgcgc cgcggcgccc aggcgcgcgc gcagcatgcg
     2401 gtcgtcgagc agcttgcgca gcacccgcgt gagcatcgcg acgtcgcccg gcacgatcag
     2461 catcccgttc acgccgtcgg cgacgatgtc cgggatgccg ccgacctcgc tcgcgaccga
     2521 cggcaggccc gccgccatcg cctcgagcag gctgatcggc aggccctcgg cgtacgaggg
     2581 caggacgaac gccgccgcgg tgtcgagcag cgcgcgcttg cgctcgggcc cgacccagcc
     2641 ggtgagctgc acgcgatcgg cgacgccgcg ctcggccgcg taggcggcga gcccgtcgac
     2701 gtcgtcgccg gcgaggacca ggcgcatgtc cggatgggtg tcgcgcagcg cggcgaccgc
     2761 atcgaccagg tcgaacacgc ccttcgcgcg ctccaggcgg ccgaggaaca gcagcgtgtt
     2821 gcggcgcccc gccgccgcgg gctccgggtg catcggcatc gggttggcga tgcagacgac
     2881 gttggggttg cgcatggcgc tcgcgagcca ggcgcgccac tgcgccgaca gcacgatgat
     2941 gcgcgaggcg cgctcgagca ggaaacgcac gatgcgcttg cgcaccgggc cgcactcctc
     3001 ctcgtagaag gtcgcgaagc cgccgccgtg caggtggaag accaccgggc agcgcgcggc
     3061 gagcgcgatc gccatgaaca ccgacttgcg ccagaagctc gcgcgcgagg cgccgtggat
     3121 atgcacgatc gcgttcggat cgcgcgccag cgacgcgacc agccggcgca cggcgcgcgc
     3181 ggcggtcgcg agcttgcgcc atcccgatcc gtcgcagtgc gtcgcgatgt actcgatcgg
     3241 ccagcgctcg aacaacccgt gggcgcggta ggtgttcacg acggccgaga tcccgccccg
     3301 cgtctcgaag gcggtgccga gcatgatgag gcgcgtgcgt gctgcggtca tggcttcagt
     3361 gggcccccgc cgtgccgagg tagcgcttga tgtccaggtg ctggacgatg agctgctgct
     3421 tgccgcgcgc ggtgcgcccg atctcgcgca ccttcacgac gcggcagtcg catcccgcct
     3481 tgtcgcccac cgcccgggcg agccgccgct gcatctccgg cgtgaagccg tcgccggcga
     3541 cgaacttcag caccagctcg cccgggcgct tctgctcgta ctgcatgctg tcgatggcgc
     3601 cgaactcggg gaagtgcgcc gagccgatct gcaccaccga gatcagccgc tcgtgccggt
     3661 cgacgatgaa ctcctgcagc cggccctcga tgcgctcgca cgcgacgaag cccgggagcc
     3721 gcgcgtggcc gcggtccgag aggatcgcca ggtcgccggt gcggtagcgc acgaacggca
     3781 tgacgcggtt gtcgaagctc gtgccgacga tctcgccggg cacgccggcc tgcgtgatcg
     3841 ggcggccgtc gagaccgacc agctcgagcc aaccgtactg cggccagaag aagtagcggt
     3901 cgtcgtcggg catcgtcgcc gccatgagca cgcgctccga gtgcccgtag tgcgcgagca
     3961 ccgggcagtg gaacacgtcg cgaaagagct tgagctggaa gccgtacacg gtctccgagt
     4021 acagcatcac gccgctcacg ttcgaggtga acgcgggcag cgggtgctcg accagccagc
     4081 gcgcgagaag gtacagcacc gacgggtacg cctcgatgaa ctgcggccgg tgcagcgcga
     4141 gcgcctccgc gtagcgcggc atgtgcgacg cgtcgaggtg cgccgagctg aagatcagct
     4201 ggcgcttgat cggctcccag gtccagaggc gtccacggtc cgcgccgggc acggggcggc
     4261 cgcggagcgc gagcgtcggc tcggtgccgt cggagccggt gcgcgcgcgg aagtcgtgca
     4321 tgaacgcgta ttccttcggc cgcgagacgt gcttctgcag gtagaactcg agcgggtcgc
     4381 ggctcgagcc gccggtgacc gtcttcaggc gcgccgactg cgggacggcg gcggacaggt
     4441 agctctccgg ccgcgccttg atcgccttct tgtccacgag cggcatgcgc gcgagcagct
     4501 ccgcggtgtc gtcgcagcga gcgaggagcg gcgcgtagtc gcggtacgcc ggcaccgtct
     4561 gcagcgccca ttcgagcgtc gaccgcagct tgcggcgcgc gaggcgctcg agcgcccccg
     4621 ggtcgcgcac cgcgacctcg tcgcggaagc ggtagtaagc gccgccgaag cggaagcgcg
     4681 cgggcaccca ggcgtaggcg cgccccgccg acgccttcac ccaggcgggc gcctgcatgt
     4741 agcggtcgag cagcggatga agcaggtcct caaaagacat gggtgtcgag cgactcgagg
     4801 acgtcggggc gaagacgcga gcggcggaag aagccgtcga tcacccgcag ctcgccggcg
     4861 cggttgagcg tgtggatgcc gagcaggcgc ggcggatacg agggcgccac gcgctcgacc
     4921 tcctcttcgg cgtagctgtc cggcgtgagc cgcagcacgc ggttgatcga cacgccggtc
     4981 ccgtacagcg gaacgccgat ctgcgccggc cggtaaaggt cctcgccgcg gcggaacagc
     5041 cgtccggcgg ggcgcgtgcc gcgcacgtcc gacttcaccg ggttgtggcg atgcggcttc
     5101 cacggaccgg ccagccggtc ggcgtggtac aggtgcagct cgtcggcgcc ttccgcgccc
     5161 gcgacggtgg cgttcgcgaa catccaccag gtgtcgccgg cgcgatggaa ggtcgagtcg
     5221 gtgaagtgca cgttctcgaa cagggtctgc tcgagtgtcc actggtcggg aaagcgcgtg
     5281 cagcggtaga cctcgaccgt gccgttgcag gcggtttcgg gaatcatcag cagctcgccg
     5341 tcctgctcga tcaggaacgg gtacgagagg tggtaggggc gctcgagcac gcgcacgggc
     5401 tgcgagcgcg ctccgtcggg cgacacttcg acagcggcga tgtgcgcctt gccgcgcgag
     5461 aacaccagct cctcgaagaa gatccagtag cggccgtcgc gcacgaccgc gaaaggatcg
     5521 gcccagaagc gatccttcgg gggcatcatc gcgacgtact ggcgcaggtc gccgcgccag
     5581 cgcgagtcgc ctccgaatct gtacgcgagg aaccactggt ccacgcacgc gagcttctgc
     5641 agcacgcggc gcgcgatgcg cgagccgagg agcgacatgc gccacagcat ccccgcggtg
     5701 ccgggaatcg acgcccggtc aagcgatgct tcctcgcgga tcggcgcgcg gctctgcagc
     5761 caggcggggc cggagcgccg cagctcgtcg agcgcgcggt aggcgaactc gccggtcttc
     5821 ggcaacagcg cgtggcgcgt gcgagcgagc gacagcggga aggtgcgcga ccacgactgg
     5881 tagatgagcc ggtcctcggc gcccggaagc cgcgcgcgca ggccggaggc ggtcacgggc
     5941 gcgccggccg ccacctcgcg cacggcggcg agcgagtcgg actcgctggc atcgggcccg
     6001 aagccgtagc gccagacgcc gtagcgcgcg acgctttcga gcgcggcgca ggtctcggcg
     6061 cccagcgcga ccaggacatc gagatccatg gcgcgaatct cggcgatcca cgcgtgcgcg
     6121 ctcgcgggcc cggagtcttc cggcggccgt ctgagcagcc gcgcctgccg cagctccgcc
     6181 gcgaggtcgg ttctctcgag aggatcggga gacgacccga acagccgcgc gtcgatggcg
     6241 cagtacgcgc gccacaggaa cggcccggcg tcgcgcgggc cgcccgcctc ggcgatgacg
     6301 acgacgtcgc cgacgcccga gtcgacgatc ttgcgcagac cgtcgacggc ccagcgcggc
     6361 tgcagcagcg agtcggcgag gacgccgatg cgcagccgcg cgggcgatcc ctcggccgcg
     6421 gtgtcgtggt cgctcgcgac gctgtcgggg ccgcgcgcga gcgctacagc cgccatacct
     6481 gcagtccctc gcgtcggaac ggatccgggt cgagcacgga cttgacgtcg atcaggcatc
     6541 cgcggcgcac gatcttctgc agataggtgg aggtcggggt ctcgaggaac tgccggtggg
     6601 cgaccgccag cacgagcgcg tcggccggcg ccaggtcgtc ccacgcgcgc aggcgcacgt
     6661 cgtactcgtg cagcgcctcc gccgcgtcgg cccacgggtc gtgcacggag atctcgacgc
     6721 cgtactccgc cagctcgcgg atgacgtcga cgaccttgga attgcggatg tcggggcagt
     6781 tctccttgaa cgtcagcccg agcacggtca cgcgcgcgcc cttgatattg cggccggcgt
     6841 tgatcatctg ctggatggtc ttgcgcgcga tgtgcggccc catgttgtcg ttgatgcggc
     6901 ggcccgcgag gatcacctcg gggtggtagc cgaccagctc ggccttgtgc gtaaggtagt
     6961 aggggtcgac gccgatgcaa tggccgccga ccagcccggg acggaacggc aggaagttcc
     7021 acttcgtgcc cgccgccttg agcacctcca tcgtgtcgat cccgagcttg tcgaagatga
     7081 tcgcgagctc gttcaccagt gcgatgttga ggtcgcgctg cgtgttctcg atcaccttcg
     7141 ccgcctcggc gacgcggatc gacgaggccg ggaacacgcc ggcgctcacg accgacccgt
     7201 acagggccgc gaccttctcc agcgtctcgg cgtcgtcgcc cgacacgacc ttgcggatcc
     7261 gggtgaagct gtgctccttg tcgcccgggt tgatgcgctc cggcgagtag ccgacgtgga
     7321 agtcctggcg ccagcgcatc ccggagtgct tctccaggat gggcacgcag acttcctcgg
     7381 tcgcgcccgg gtacacggtc gactcgtaga ccacgatcgc gccgggcttc atgtgctcgc
     7441 cgacggtgcg gctcgcagac tcgagcggcg agaagtccgg ctgccgcgcg gcgttcaccg
     7501 gcgtgggcac cgcgacgatg atgtagtcgg cttccgcgag gatcgccgga tcgtcgctga
     7561 cgcgcagctt ctccgcctgg cgcagctcct cgggcgccac ctcgcccgtg gtgtcgatgc
     7621 cgtggcgcag attcgcgatc ttcttgcgcg acagatcgta gccgaccgtc ggccggcgct
     7681 tgccgaactc gacggcgacc gggaggccga cgtagccgag gccgacgacc gccacggtcc
     7741 ccagttcgga ctgcgcctcg gcgcgcgttt cggaaacttc atcggaccgg agcgagagct
     7801 ccggcttgtc ttcgactagc agcattgctt gaggtcccct gccgaatgga cgcctcattt
     7861 tgaggggggg atgttaagaa cgattgatcg ccgaaggacc tatcgggcta tcgcggtcgt
     7921 gctcctcgac ttcgacgcct tctcgggatg ctcggcgcgg cgtccgccca gattggcgac
     7981 gtaccagtcg atcgcggcgg cgaggccgga gcgcacatcg atccgcggct cgtacccg
//
LOCUS       DATDMW010000217        13214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_562897, whole genome shotgun sequence.
ACCESSION   DATDMW010000217 DATDMW010000000
VERSION     DATDMW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13214
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562897"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VI319_09455"
     CDS             complement(<1..221)
                     /locus_tag="VI319_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864114.1"
                     /translation="MSRVNRLWSKLRMVGLLLAAAGAALGLAPRDWRSAGAAPAGLAP
                     DPAATPEAVVQVYGARVSGWRGIFGVHTWL"
     gene            284..475
                     /locus_tag="VI319_09460"
     CDS             284..475
                     /locus_tag="VI319_09460"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEY6864115.1"
                     /translation="MAARPQAQDYVPPDPTRIDVRDPRALGFWSATLGKSADEIRAAV
                     AKVGPLLEHVKSELGMAGE"
     gene            737..1153
                     /gene="rpsF"
                     /locus_tag="VI319_09465"
     CDS             737..1153
                     /gene="rpsF"
                     /locus_tag="VI319_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY6864116.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMIERYRSTVTSRSGKIHRLEDWGR
                     RQLAYPLAKVHKAHYVLMNIECDSATLGELEHSFKFNDAVLRHLIVKMERAWTTPSPM
                     MKEEKAKSVLNPGEAKSQDGARAAEPAKPAEPQPAA"
     gene            1158..1466
                     /gene="priB"
                     /locus_tag="VI319_09470"
     CDS             1158..1466
                     /gene="priB"
                     /locus_tag="VI319_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEY6864117.1"
                     /translation="MAQANRIVLGGRIVELDVLRHTPAGVPLLRFRLSHESSLAEAGS
                     ERKVDCEIAGVAFEREAKLLASARLGSAVTVTGFLAARSRTSRQPVLHATHIEFTEGD
                     "
     gene            1469..1762
                     /gene="rpsR"
                     /locus_tag="VI319_09475"
     CDS             1469..1762
                     /gene="rpsR"
                     /locus_tag="VI319_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY6864118.1"
                     /translation="MPPPRGKGRGKGKGRDGKRDGKGSKALFKRRKFCRFTAEKMEWI
                     DYKDVDLLKDFIGENGKIIPARITGTRTHYQRQLSDAVKRARFLALLPYTDLH"
     gene            1772..2227
                     /gene="rplI"
                     /locus_tag="VI319_09480"
     CDS             1772..2227
                     /gene="rplI"
                     /locus_tag="VI319_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY6864119.1"
                     /translation="MQVILLEKVVNLGGLGDVVKVKDGYARNFLLPQGKAKRATPENL
                     AEFEKKRAELERAQNEALAHAQEKGAKLDGLMLQITQKAGVDGRLFGSVTNYDIAEAL
                     QKQGFEVERSMIRLPQGPLKQVGDHEIAIALHGDVVVHVKVAVLGETAQ"
     gene            2577..3965
                     /gene="dnaB"
                     /locus_tag="VI319_09485"
     CDS             2577..3965
                     /gene="dnaB"
                     /locus_tag="VI319_09485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY6864120.1"
                     /translation="MAQPAPQSVQDPQLLQLKLPPHSIEAEQSVLGGLLIDNAAFDKI
                     ADIVSDPDFYRDDHRRIFRHIARLIEKGKPADVITVDESVKSSEDKDKVGGLAYLAAL
                     TGNTPSAHNIRRYAEIVRERAIMRKLVEVGTNIADTALNPMGKEVGQLLDEAESRVFE
                     IAEAGSRGRQGFIEIQPLLTQVMERIDMLYHRDNPSDITGVPTGYTDLDSRTSGLQPG
                     DLVIVAGRPSMGKTALALNMAEYVAVENRLPVAVFSMEMAGTQLAMRMLGSIGRLDQH
                     KLRTGKLSDEDWNRLTTAVGKLHDAPIHIDETAALNALELRARARRLHRQYGALGLIV
                     IDYLQLMEASTQGENRATEISEISRSLKALAKELKVPVVALSQLNRGLEQRPNKRPVM
                     SDLRESGAIEQDADLILFIYRDEVYNPDTPEKGIAEIIIGKQRNGPIGTVKLTFLGEY
                     TRFESHADSGKF"
     gene            3970..4908
                     /locus_tag="VI319_09490"
     CDS             3970..4908
                     /locus_tag="VI319_09490"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY6864121.1"
                     /translation="MLRLLAAVLAAGWLQSAIAQIPASYSGPEQPGTVAGRVELIEGD
                     VRVLDGNRNVRHPKLGDEIHAGESIVTGADGEVHLDMEDGGYMGVRPNTNLRFAEFRA
                     DGSDADKSVLDLFEGSFRAVTGWIAKLGAKSYAVRTPTVTIGVRGTEHEPMVIPEGSR
                     LGEAGTYERVHIGETFMQTPQGMVTVRPNQAGFAPHRGALRPRLLDRVPAFFRPTRNE
                     ARFGGLHARLQARMQQRREQRRLFLQERRKSGGLIRGPQSAPLRERQEARREQLQKMR
                     AQKQQVQERREAAAKSRAARKHDEAGEHPRRRREGP"
     gene            complement(4905..5531)
                     /locus_tag="VI319_09495"
     CDS             complement(4905..5531)
                     /locus_tag="VI319_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY6864122.1"
                     /translation="MPPRASLAPSLLSLLALVIAPAALAQQGGEPARELPALQLVDEM
                     RAGGRVLFLRHAATDFTENDSRMAGFEDCAHQRNLVDKGRDDARKIGIAIRALGIPIG
                     RVMASPYCRTVETAELAFGRAEKMNEARYATGEGAERYADLRKQLGTRPAKGNSVIVG
                     HGTPFYALTGVRLVEGEVAVIEPLDGRFEILGRIKPDAWGALRNAARR"
     gene            complement(5593..6075)
                     /gene="bfr"
                     /locus_tag="VI319_09500"
     CDS             complement(5593..6075)
                     /gene="bfr"
                     /locus_tag="VI319_09500"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY6864123.1"
                     /translation="MQGDRKVIEHLNTVLTNELTSINQYFLHSRMFRNWGLEKLARHE
                     YEESVDEMKHADRLIERILFLEGLPNLQQLNNLLIGENVREALECDLKLEMGALPMLK
                     SAIAYCESTADYVSRELFEDILESEEDHVDWIETQIDLIAKIGVENYLQSQMGGAAAS
                     "
     gene            complement(6155..6355)
                     /locus_tag="VI319_09505"
     CDS             complement(6155..6355)
                     /locus_tag="VI319_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6864124.1"
                     /translation="MYICICNGITDRDIRQAAAAGASTLNDLRRELGVGAGCGCCASC
                     AHAVLCESCPAGDVPSPSLQPA"
     gene            6455..7681
                     /locus_tag="VI319_09510"
     CDS             6455..7681
                     /locus_tag="VI319_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY6864125.1"
                     /translation="MRRKSIALALAAAFAAPAFAQQPTVEQKLQILQQEIDELKAQSR
                     SASPPDASIGLRAGATTIVGYGEFNYNRFRQDDHETKADLRRFVLGLDHRFDDRLSFH
                     SEVEVEHAVVSKNDDGEFEIEQAWLDYRLTDGDRVNVKGGLFLIPLGILNETHEPPTY
                     YGVERNEVETRIIPTTWRELGVGVHGAVAPGLRYDVGVTTGFDAGKIDDPSTGIRSGH
                     QEGQQANAKDLSVYGALNYRAPGLLVGGGVFTGNTGQNGQSNPLLKGVDARLTLWDVH
                     ARYRVAGFDLQALYAAGKLGDTDRINAANVAAGQTFAAPKSMKGAYVQAAYHVYKRGS
                     FDVAPFVRLERYDIRQEEDPADNLIQNELIRNERVRTIGVNFWVHPQVVIKADVQRFR
                     NDKSLDRFDLGLGYMF"
     gene            7681..8199
                     /locus_tag="VI319_09515"
     CDS             7681..8199
                     /locus_tag="VI319_09515"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEY6864126.1"
                     /translation="MRAAAVICLALLAAALLALGPARAEVIQDPDAFVGEVFGGRPAP
                     KVLWLTPDIQAEATAILEHPPAQLRQRYWSDARRSLWILEEIGKEEPITAGFVVADGR
                     IEHVRVLVYRESRGGEVREPAFVKQFQGAKLAKSNRLDRDIDGIVGATLSVGAMQRMA
                     RLALYFDRVSRR"
     gene            8200..8937
                     /locus_tag="VI319_09520"
     CDS             8200..8937
                     /locus_tag="VI319_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEY6864127.1"
                     /translation="MSPRLHFLRLLRRWHARIGFIAAAFFILLAVTGVVANHGDALGL
                     DGARVHSEWLDHWYGIALEPPRAAFRAGSHELVAANGRWMLDARPAGDERPQPLGFVE
                     LGDMLVVACAGSLYVLRADGRLVDRLEGRALPAVPIESVGTEGGELLLRTRSGTFASA
                     DVMSWQSARHADVSWSAPVALSDDERERYGAQLAPGIPMQRLLLDVHSGRIAGRYGPL
                     AVDAIALVLVALAASGAWMFASHRRRR"
     gene            8943..9995
                     /locus_tag="VI319_09525"
     CDS             8943..9995
                     /locus_tag="VI319_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY6864128.1"
                     /translation="MPRARPGMWLLWLCAALALRPAAGAEYRAASYVFGTIARITIAG
                     DDADAARGAAGAVLHEFDRLHRALHAWQPGPLVDLNAAIARGERTIRTTPEIAELIVR
                     AQRLEALSDGAFDPAIGRLVALWNFHRDSPGGPLPDASDLSRLVAARPRMADLRVEND
                     VVTSTNPLVQLDLGGYAKGYALDRAAQILRARGVADALVDVGGNVMALGTAESRPWRV
                     AIEAPRGGGTLGTVDLHDGEAMGTSGDYRRYYEIAGRRYAHIIDPCTGYPVRGVESVT
                     VLVAPGPDAGALSDAASKPIFIAGREGWRQAARRMGVAGALLVDSDGVAHMTAPLRAR
                     LRAAGAGKRGGRYATR"
     gene            complement(9980..10885)
                     /locus_tag="VI319_09530"
     CDS             complement(9980..10885)
                     /locus_tag="VI319_09530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY6864129.1"
                     /translation="MTWRKHDVSEVIEQVPIGVAVTLPLGTVEYANPRLLELLQIDAG
                     RVLGALLRDFRASASASRDHAIRRRLCAGASWQGETQLRTAGGEVRHVLESVFPLRDR
                     AGRLTHFIHFLQDMGALEAAETLSSLAYYDSLTGLPNRNLFNDRLSRAIAAARRRHGG
                     FSLLYVDVDRFKDVNDTRGHDAGDELLRQVALRLQKSLRKIDTVARLGGDEFVVILDQ
                     VSDDAQAAKIAAKILASCSGEYELEGAACNVTLSLGISRYPRDAVEVESLVKCADKAM
                     YRAKASGGNDFRLREPAHRHRYRVA"
     gene            11098..11454
                     /locus_tag="VI319_09535"
     CDS             11098..11454
                     /locus_tag="VI319_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864130.1"
                     /translation="MRRARSESESPTRIAREFNLRYDGDPVTAQAVRKWLDGRALPSQ
                     DKVRALASWLEVSPQWLRFGGEDRKDGHALARQETASYNVDHAWVAKKFELLGDQHKK
                     MVLEMVRALLRLEGKQ"
     gene            complement(11473..12903)
                     /gene="boxB"
                     /locus_tag="VI319_09540"
     CDS             complement(11473..12903)
                     /gene="boxB"
                     /locus_tag="VI319_09540"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEY6864131.1"
                     /translation="MTAIDYSEKIPNNVDLAHDRALQRALEHWQPRFLDWWREMGPSD
                     FAGADVYLRTAVGVDAQGWAHYGTVKMPDYRWGIFLADAIPDRRIGFGDALGQPVWQQ
                     VPGEFRSQFRRLIVTQGDTEPASVEQQRRLGLTCPSLYDLRNLFQVNVEEGRHLWAMV
                     YLLHAYFGRDGREEAEELLARHSGDVDKPRILGTFNEPIDDWLSFFCFTYFTDRDGKF
                     QLKSLAESAFDPLARTCRFMLTEEAHHMFVGETGIGRVVRRALEAMKEAGSDDPDAVR
                     RRGAIDLPLLQRYLNFWFSSSLDLFGSEVSSNAASTFATGIKGRPDETTQYPDHVCAD
                     AAFELEQPDGRGGVAREPVPMRNAMNEVVRAAYVKDCEIGVKRWNLQIQRAGHDFGLS
                     LPSPRFRRGIGVWAGVATDLEGRPIDRTEFEHRLPQWLPSAEDKAYVRGLMQRVAEPG
                     KMAAWIAPPDRGINNLPLDYEYVRLS"
     gene            complement(12900..>13214)
                     /locus_tag="VI319_09545"
     CDS             complement(12900..>13214)
                     /locus_tag="VI319_09545"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745258.1"
                     /note="cleaves the ring of
                     2,3-dihydro-2,3-dihydroxybenzoyl-CoA forming
                     6-hydroxy-3-hexenoyl-CoA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA-dihydrodiol lyase"
                     /protein_id="HEY6864132.1"
                     /translation="RIGHKLDARLARELGLVTFTPDDLDWDDEVRIAIEERASLSPDA
                     LTGLEANLRFTGKETMASRIFGRLSAWQNWIFNRPNAVGDHGALKVYGTGAKAKFNRE
                     RV"
BASE COUNT         2159 a         4431 c         4627 g         1997 t
ORIGIN      
        1 agccaggtat gcaccccgaa gatcccgcgc cagcccgata ccctggcgcc gtagacctgg
       61 accacggctt cgggggtcgc cgcgggatcg ggcgcaagtc ccgccggcgc tgcgcccgcg
      121 ctgcgccaat cgcgcggcgc cagcccgagc gcggccccgg ccgcggccag cagcaatccc
      181 accatgcgca atttgctcca cagcctgttc accctgctca ttgcccgtta cctctggtct
      241 gaattcgcga gaacacacgg cgaaagcggc ggtcccatcc gggatggcag cccgacccca
      301 ggcgcaggat tacgtcccgc cggacccgac gcgcatcgac gtgcgcgacc cgcgtgcgct
      361 aggcttctgg tccgccaccc tcggcaagtc ggcggacgag atacgcgcgg cggtcgcgaa
      421 ggtggggccg ctgctcgagc acgtgaagag cgaattgggg atggcgggcg aataagcgcc
      481 ccggcggagc ggttgcgctg gacctttagg accacggctg tcatacccgt aacagaaacc
      541 cggtttatcc gtgacacagc cgcagcgtag tttgtaagtg ttacaaaccc atggcgcgtc
      601 tgttacaatt ccgtgttccg ggatggcggt ttcgtggtcg ttccggttca tacaattcgt
      661 tcaaccctgc cgcaccggca gtcgtcgggt cgcaacggcg atgtgagggc ggctacaaac
      721 catagggaat caaggcatgc ggcattacga gatcgttttc atcgttcacc cggaccagag
      781 cgagcaggtc ccggcgatga tcgagcgcta ccgttccacg gtcacctcga ggagcggaaa
      841 aatccatcgg ctggaagact gggggcggcg ccagctcgcg taccccctcg ccaaggttca
      901 caaggcccac tacgtgctca tgaacatcga gtgcgactcg gccacgctgg gcgagctcga
      961 gcactcgttc aagttcaacg acgcggtcct gcgccacctc atcgtgaaga tggagcgcgc
     1021 ctggaccacg ccttcgccga tgatgaagga agagaaggcg aagtcggtgc tcaaccccgg
     1081 cgaggcgaag tcccaggacg gcgcccgggc cgcagagccg gcgaagccgg ccgagccgca
     1141 gcccgcagcc tgaggccttg gcgcaagcga accgcatcgt tctcggcggc cgaatcgtgg
     1201 agctcgacgt cctgcgccac acgccagccg gcgtgccgct gctcaggttc cgcctgagcc
     1261 atgagtcgtc gctcgccgag gcggggagcg agcgcaaggt cgactgtgag atcgcgggcg
     1321 tcgccttcga gcgtgaagcg aagctgctcg ccagcgcgag gctcggaagc gcggtcacgg
     1381 tcaccggttt cctcgcggcc aggagccgca ccagccggca gcccgtgctg cacgcaacgc
     1441 acatcgaatt tacggaagga gattagcaat gcctccaccc agagggaaag gccgcggcaa
     1501 aggcaagggc cgcgacggta aacgcgacgg aaaaggctcg aaggcgctgt tcaagcggcg
     1561 caagttctgc cgcttcaccg ccgagaagat ggagtggatc gactacaagg acgtcgacct
     1621 gctcaaggat ttcatcggcg agaacggcaa gatcatcccg gcacgcatca ccggcacgcg
     1681 cacccactac cagcgtcagc tctccgacgc ggtgaagcgg gcgcggttcc tggcgctgct
     1741 gccgtacacc gacctgcact gaaggaccgc catgcaagtg atattgctgg aaaaggtagt
     1801 gaacctgggc ggcctcggcg acgtggtcaa ggtcaaggac ggctacgcgc gcaatttcct
     1861 gctgccgcag ggcaaggcca agcgcgccac gcccgagaac ctcgcggagt tcgagaagaa
     1921 gcgcgccgag ctcgagcgcg ctcagaacga ggcgctcgct catgcgcagg agaagggcgc
     1981 gaagctcgac ggcctgatgc tgcagatcac gcagaaggcc ggcgtcgacg ggcgcctgtt
     2041 cgggtcggtc acgaactacg acatcgccga ggcgctgcag aagcagggct tcgaggtcga
     2101 gcgctcgatg atccgcctgc cgcaagggcc gctgaagcag gtcggcgatc acgagatcgc
     2161 gatcgcgctg cacggcgacg tggtggtgca cgtcaaggtg gcggttctgg gagagacggc
     2221 gcagtagtca caggccgcgc acaagctgcg cacagacaat ccacaggtaa tccaacgcga
     2281 ggggcttcgg cccctttcgt tttttgttgc tgtcgccgcg tcaatttctc cgacgaagcc
     2341 gggatcgttt cttgcagcag cgagtgcgcg cgcgcgacgt ggcgcgcgag caacagattc
     2401 gcgaaaaatt ttcgcgcgcc cttgctgcgg tccgcggttc gcggtataaa acctccgcgt
     2461 tacacgtcgg aaaaaagagc ggggaggggt gacgtggcag tccacgttgg cagtcattgt
     2521 tcgcggactg aggtcttcca ccgtggtggc cggtccgcgg cccgcctggc ccgctgatgg
     2581 cgcagcccgc tccccagtcc gtgcaggacc cgcagctgct gcagctcaag ctgccgccgc
     2641 actcgatcga ggccgagcag tcggtcctcg gcggcctgct gatcgacaac gcggccttcg
     2701 acaagatcgc cgacatcgtc tcggacccgg acttctaccg cgacgaccac cgccgcatct
     2761 tccggcacat cgcgcgcctg atcgagaagg gcaagccggc ggacgtcatc accgtcgacg
     2821 agtcggtcaa atcgagcgag gacaaggaca aggtcggggg gctcgcctac ctggcggccc
     2881 tgaccgggaa cacgccgtcg gcgcacaaca tccgccgcta cgcggagatc gtgcgcgagc
     2941 gcgccatcat gaggaagctg gtcgaggtcg gcacgaacat cgccgacacc gcgctcaacc
     3001 ccatgggcaa ggaggtcggg cagctcctgg acgaggccga gtcgcgcgtg ttcgagatcg
     3061 ccgaggccgg ctcgcgcggc cggcagggct tcatcgagat ccagccgctg ctcacgcagg
     3121 tgatggagcg catcgacatg ctctatcacc gcgacaatcc ctcggacatc accggggtgc
     3181 cgaccggcta caccgacctc gactcgcgca cctcgggcct gcagccgggc gacctggtga
     3241 tcgtcgccgg ccgcccgtcg atgggcaaga ccgcgctcgc gctcaacatg gccgagtacg
     3301 tcgcggtgga gaaccggctt ccggtcgcgg tcttcagcat ggaaatggcg ggaacgcagc
     3361 tcgcgatgcg catgctgggt tcgatcggtc ggctcgacca gcacaagctg cgcaccggca
     3421 agctgtcgga cgaggactgg aaccgcctca cgaccgcggt cggtaagctg cacgacgcgc
     3481 cgatccacat cgacgagacg gcggcgctga acgcgctcga gttgcgcgcg cgcgcgcggc
     3541 gcctgcaccg gcagtacggc gcgctcggct tgatcgtgat cgattacctg cagctcatgg
     3601 aggcgtcgac ccagggggag aatcgcgcca cggaaatctc cgagatctcg cgctcgctca
     3661 aggcgctcgc caaggagctc aaggtcccgg tggtggcgct gtcgcagctg aaccgcggcc
     3721 tcgagcagcg gccgaacaag cgacctgtga tgtcggacct gcgcgagtcg ggcgcgatcg
     3781 agcaggacgc cgacctcatc ctcttcatct accgtgacga ggtctacaac ccggacacgc
     3841 cggagaaggg catcgccgag attatcatcg gcaagcagcg aaacgggccc atcggcacgg
     3901 tgaagctcac gttcctcggc gagtacacgc gcttcgagag ccacgccgac tccggaaagt
     3961 tctgagaccg tgctgcgcct gctcgcagcg gtgctggcgg cgggctggct ccaatcggcg
     4021 atcgcccaga ttcccgcgtc gtattccggt cccgagcagc cgggcacggt cgcgggccgc
     4081 gtggagttga tcgaaggcga cgtgcgcgtc ctggacggca accgcaacgt ccggcatccg
     4141 aagctcggcg acgagatcca cgccggggaa agcatcgtca ccggcgccga cggcgaggtg
     4201 catctcgaca tggaggacgg cggctacatg ggcgtgcggc ccaacacgaa tttgcgcttc
     4261 gccgagttcc gcgccgacgg ctcggatgcc gacaaatcgg tgctcgacct gttcgaaggc
     4321 tcgtttcgcg cggtcaccgg ctggatcgcg aagctcggcg cgaagagcta cgcggtgcgc
     4381 acgccgacgg tcacgatcgg cgtgcgcggc accgagcacg agcccatggt gattcccgaa
     4441 ggcagccggc tgggcgaggc gggcacctac gagcgcgtgc acatcggcga gacgttcatg
     4501 cagacgccgc agggcatggt gaccgtgcgt cccaaccagg cggggttcgc gccgcaccgc
     4561 ggggcgctgc ggccgcggct gctcgatcgc gtgccggcat tcttccggcc gacgcgcaac
     4621 gaggcgcggt tcggcggcct gcacgcgcgg ctgcaggcgc gcatgcagca gcggcgcgag
     4681 cagcggcggc tgttcctcca ggaacgccgc aagagcggag gcctgatccg cggtccgcag
     4741 tccgcgcccc tgcgcgagcg gcaggaagcg aggcgcgagc agctgcagaa gatgcgcgcg
     4801 cagaagcagc aggtccagga gcgacgcgag gcggcggcca agtcgcgcgc cgcccgcaag
     4861 cacgacgagg ccggggagca tccgcgccgg cggcgcgagg gaccctagcg ccgggcggcg
     4921 ttgcgcagcg cgccccaggc gtcgggcttg atccgcccga ggatctcgaa gcggccgtcg
     4981 agcggctcga tcaccgcgac ttcgccctcg accaggcgta cacccgtcag ggcgtagaac
     5041 ggcgtgccgt gcccgacgat caccgagttg cccttcgccg gccgcgtacc gagctgcttg
     5101 cgcagatcgg cgtagcgctc ggcaccttcg ccggtcgcgt agcgcgcctc gttcattttc
     5161 tccgcgcgtc cgaacgcgag ctccgcggtc tcgacggtgc ggcagtaggg gctcgccatg
     5221 acgcgcccga tcgggatgcc gagcgcgcgg atcgcgatgc cgatcttgcg cgcgtcgtcg
     5281 cgacccttgt ccacgaggtt acgctggtgc gcgcagtcct cgaagcccgc catgcgcgag
     5341 tcgttctcgg tgaagtcggt ggcggcgtgg cgcaggaaca gcacccggcc gcccgcgcgc
     5401 atctcgtcga cgagctgcaa cgcgggtagc tcgcgcgccg gctcgccgcc ctgctgggcg
     5461 agcgcggccg gcgcgatcac gagagcgagg agcgacagca gcgacggcgc gagcgaagcg
     5521 cgggggggca tgggtcgtcc atccgtgggg tgatggaggc gacgattgta cgcgcgtggc
     5581 tggccgccgg ggtcaggagg cggccgcgcc gcccatctgc gactgcaggt agttctcgac
     5641 cccgatcttg gcgatgaggt cgatctgggt ctcgatccag tcgacgtggt cttcctcgga
     5701 ttcgagaatg tcctcgaaca gctcgcgcga gacgtaatcg gccgtgctct cgcagtacgc
     5761 gatcgcgctc ttcagcatgg gaagcgcgcc catctcgagc ttgaggtcgc actcgagcgc
     5821 ctccctgacg ttctctccga tcagcagatt gttgagctgc tgcaggttcg gcagcccttc
     5881 gaggaacagg atgcgctcga tcaggcggtc ggcgtgcttc atctcgtcga ccgattcctc
     5941 gtactcgtgg cgggcgagct tctcgaggcc ccagttgcgg aacatgcgcg agtgcaggaa
     6001 gtactggttg atcgaggtga gctcgttcgt gagaacggtg ttgaggtgct cgatgacttt
     6061 cctgtcgcct tgcatgcggc ctccgggaaa tcaccaaacg atagcaccgg cgtcgtcttg
     6121 ccgtatggcc ggcgcgtcgc cgggcgatgc ggtcctacgc gggctgcagc gagggcgacg
     6181 ggacgtcgcc ggcagggcag ctctcgcaca gcacggcgtg ggcgcaggaa gcgcagcagc
     6241 cgcagcccgc gcccacgccg agctcgcggc gcaggtcgtt cagcgtgctc gcgccggcgg
     6301 cggccgcttg gcggatgtcg cgatcggtga tgccgttgca gatgcagatg tacatgggcg
     6361 ctcggcgctt gtaatgcgaa ctattctcat ttatattccc gccgtggtca agagggtcag
     6421 gcctttcgga cttgacgcaa gtcaaagagg agccatgaga agaaaaagca tcgcgctggc
     6481 gctggcggcc gcattcgccg cgcccgcgtt cgctcagcag cccactgtgg agcaaaagct
     6541 tcagatcctg cagcaggaga tcgatgagtt gaaggcgcag tcacgcagtg catcgccgcc
     6601 cgacgccagc atcgggctgc gcgccggcgc gaccaccatc gtcggctacg gcgagttcaa
     6661 ctacaaccgc ttccgccagg acgaccacga gaccaaggcc gacctgcggc gcttcgtgct
     6721 cgggctcgac caccgcttcg acgatcgcct gagcttccac tcggaggtcg aggtcgagca
     6781 cgcggtcgtc agcaagaacg acgacggcga attcgagatc gagcaggcgt ggctcgacta
     6841 ccgcctgacc gacggcgatc gcgtcaacgt caagggcggt ctgttcctca tcccgctcgg
     6901 aatcctgaac gagacgcacg agccgccgac gtactacggc gtcgagcgca acgaggtgga
     6961 aacgcggatc atcccgacga catggcgcga gctcggcgtg ggcgtgcacg gcgcggtcgc
     7021 gccggggctg cgctacgacg tcggcgtcac caccggcttc gacgccggga agatcgacga
     7081 tccgtccacg gggatccgca gcgggcacca ggaaggccag caggccaatg ccaaggacct
     7141 gagcgtgtac ggcgctctga attaccgcgc gcccgggctg ctcgtgggcg gcggggtctt
     7201 caccgggaac acggggcaga acgggcagag caacccgctg ctgaagggcg tcgatgcgcg
     7261 tctcacgctg tgggacgtgc acgcgcgata ccgcgtcgcc ggcttcgacc ttcaggcact
     7321 ctacgccgct ggcaagctcg gcgacacgga ccgcatcaac gcggcaaacg tcgccgcagg
     7381 gcagaccttc gccgcgccca agtcgatgaa aggcgcctac gtccaggccg cgtaccacgt
     7441 ctacaagcgg ggtagtttcg acgtcgcgcc ctttgtgcgg ctcgagcgct acgacatccg
     7501 gcaggaggag gatccggcgg acaacctgat ccagaacgag ctgatccgca acgagcgcgt
     7561 gcgcacgatc ggcgtcaact tctgggtgca cccgcaggtc gtgatcaagg ccgacgtgca
     7621 gcgcttccgc aacgataaga gcctcgaccg gttcgatctc ggtctcggct acatgttctg
     7681 atgcgcgcgg cggccgttat ctgtctcgcg ctgcttgcgg cagctctcct cgcgctcggc
     7741 cccgcgcgag ccgaggtgat ccaggatccc gacgccttcg tcggcgaggt cttcggcggg
     7801 cgcccggcgc cgaaggtgct gtggctcacg cccgatatcc aggccgaggc cacggcgatc
     7861 ctcgaacatc cgcccgcgca gttgcgccag cgctactggt ccgacgcgcg ccgcagcctc
     7921 tggatcctgg aggagatcgg caaggaggag ccgatcacgg cgggcttcgt cgtcgccgac
     7981 ggacgcatcg agcacgtgcg cgtgctggtc tatcgcgaaa gccgcggcgg tgaggtgcgc
     8041 gagccggcgt tcgtgaagca gttccagggt gcaaagctcg cgaagtccaa tcgtctcgac
     8101 cgcgacatcg acggcatcgt cggcgcgacg ttgtcggtgg gcgcgatgca gcgcatggcg
     8161 cggctcgcgc tctacttcga ccgcgtgagc aggcgataga tgtcgccgcg actgcatttc
     8221 ctgcggctgc tgcggcgctg gcacgcgcgc atcggcttca tcgccgcggc ctttttcatt
     8281 ctgctcgcgg tcacgggcgt cgtcgccaac cacggcgatg cgctcgggct ggacggcgca
     8341 cgcgtccaca gcgaatggct cgaccactgg tacgggatcg cgctcgagcc gccgcgcgcg
     8401 gcgttccgcg ccggatcgca cgagctggtc gccgcgaacg gccgctggat gctcgacgcg
     8461 cgtcccgcgg gcgacgagcg gccacagccg ctcggcttcg tcgagctggg cgatatgctg
     8521 gtcgtcgcct gcgcgggctc gctgtacgtg ctgcgcgccg acggcaggct ggtcgaccgc
     8581 ctcgaggggc gcgcgctgcc ggcggtcccg atcgaatcgg tgggcacgga gggcggcgag
     8641 ctgctgctgc gcacccgctc cgggacgttc gcgagcgcgg acgtgatgtc gtggcagtcg
     8701 gcgcggcatg cggacgtctc gtggtccgcg ccggtcgcgc tctcggatga cgagcgcgag
     8761 cgctacggcg cgcagctcgc acccggaatc ccgatgcagc gattgctgct cgatgtccac
     8821 agcggacgca tcgccggacg ctacggcccg ctcgccgtcg acgcgatcgc cctggtgctg
     8881 gtggcgctcg cggcgagcgg cgcgtggatg ttcgcctcgc accgccgccg gcgctgaccc
     8941 gcatgccgcg cgcacgtccc ggaatgtggc tgctgtggct gtgcgccgcg ctcgcgcttc
     9001 ggccggccgc gggcgcggag tatcgcgccg cgtcatacgt gttcgggacg atcgcaagga
     9061 tcaccattgc cggcgacgac gcggacgccg cgcgcggcgc cgccggcgcg gtgctgcacg
     9121 aattcgatcg cctgcatcgc gcgctgcacg cctggcagcc gggaccgctc gtcgatctga
     9181 acgccgcgat cgcacgcggc gagcggacga tccggaccac tcccgagatc gccgagctga
     9241 tcgtgcgtgc gcagcgcctc gaggcgctct ccgacggcgc gttcgacccg gccatcgggc
     9301 gcctggtggc gctctggaac ttccatcgcg actcgcccgg cgggccgctt ccggacgcgt
     9361 ccgacctctc gcgcctcgtg gccgcgcgtc cgcgcatggc ggacctgcgc gtcgaaaacg
     9421 atgtcgtgac cagcaccaat ccgctcgtgc agctcgacct cggcggctac gccaagggat
     9481 atgcgctcga ccgcgcggcg cagatcctgc gcgcgcgcgg cgtggcggac gccctcgtcg
     9541 acgtcggcgg caacgtgatg gcgctcggca ccgcggagag caggccctgg cgcgtggcga
     9601 tcgaggcgcc ccgcggcggc ggcacgctcg gcacggtcga tctgcacgac ggcgaggcga
     9661 tggggacctc cggggactat cgccgctact acgagatcgc ggggcggcgc tatgcgcaca
     9721 tcatcgaccc gtgcaccggc tatcccgtgc gcggcgtcga atcggtcacg gtgctggtgg
     9781 cgccggggcc ggacgcgggc gcgctctcgg acgcggcgtc gaagccgatc ttcatcgcag
     9841 gacgcgaagg ctggcgccag gcggcgagac gcatgggcgt cgcgggcgcg ctgctcgtcg
     9901 attcggacgg agtcgcgcac atgacggcgc cgctgcgggc acggctgcgc gccgcgggcg
     9961 cggggaaacg gggcggccgc tacgcgacgc ggtagcggtg gcggtgcgct ggctcgcgca
    10021 gccggaagtc gttgcccccg gaagccttcg ctcggtacat cgccttgtcg gcgcatttca
    10081 cgagcgattc gacctcgacc gcgtcgcgcg gataacggct gatgccgagg ctcagcgtga
    10141 cgttgcacgc cgcgccctcg agctcgtact cgccgctgca cgaggcgaga atcttggcgg
    10201 cgatcttcgc cgcctgcgcg tcgtcggaga cctggtcgag gatgacgacg aactcgtcgc
    10261 cgcccaggcg cgcgacggtg tcgatcttcc gcagcgactt ctggagacgc agcgcgacct
    10321 gccgcagcag ctcgtcgccc gcgtcgtggc cgcgcgtgtc gttcacgtcc ttgaagcggt
    10381 cgacgtcgac gtagagcagc gagaagccgc cgtggcgccg gcgcgcggcc gcgatcgcgc
    10441 gcgacagccg gtcgttgaag agattgcggt tgggcagccc cgtgaggctg tcgtagtacg
    10501 caaggctgga gagcgtctcg gcggcctcga gcgcccccat gtcctgcagg aagtggatga
    10561 agtgcgtgag ccgcccggcg cggtcgcgca gcgggaacac cgactccagc acgtgacgca
    10621 cctcgccgcc cgcggtgcgc agctgcgtct cgccctgcca cgacgcgccg gcgcacaggc
    10681 ggcggcggat cgcgtggtcg cgcgacgcgc tggcggacgc acggaaatcg cgcagcaggg
    10741 cgccgagcac gcgcccggcg tcgatctgca gcagctcgag cagccgggga ttggcgtatt
    10801 cgaccgtgcc cagcggcagc gtcaccgcca cgccgatggg gacctgctcg atcacctccg
    10861 agacatcgtg cttgcgccag gtcatcgcac atttcttcga cgagggcgag gctgagaatg
    10921 cgggcgggac cgcaggcatc gggcccggcc gcgacgtggg gtttcgcatg aatggtaccg
    10981 atagttacct ttcaaggcaa ctaccggtgg tcgtctcctc tcgcgcttgg tgatactttg
    11041 ccaagcatga ccatcgccga tgaaaaactc agtttcagca aacgtctcaa agaggctatg
    11101 cgccgggcgc gctcggagag cgagagcccg accagaatcg cgcgcgagtt caatctccgt
    11161 tacgacggcg acccggtgac cgcccaggcc gtgcgcaagt ggctcgacgg gcgcgcgctg
    11221 ccgtcgcagg acaaggtgcg cgcgctcgcc tcctggctgg aggtctcgcc gcagtggctg
    11281 cgcttcggcg gagaggaccg caaggacggc cacgcgctgg cgcgccagga gacggcgagc
    11341 tacaacgtcg accacgcctg ggtggcgaag aagttcgagc tcctcggcga tcagcacaag
    11401 aagatggtgc tggagatggt ccgcgccctg ctgcgcctcg agggcaagca gtagccgggc
    11461 ggaggacccc gactagctca gtcggacgta ctcgtagtcc agggggaggt tgttgatgcc
    11521 ccgatccggc ggggcgatcc aagccgccat cttccccggc tcggcgacgc gctgcatcag
    11581 cccgcgcacg taagccttgt cctcggcgct cgggagccat tgcggcaggc ggtgctcgaa
    11641 ctccgtgcgg tcgatcggcc ggccttcgag gtccgtcgcc acgccggccc agacgccgat
    11701 gccgcgccgg aagcgcggac tgggaagcga gaggccgaaa tcgtggcccg cgcgctggat
    11761 ctgcaggttc cagcgcttga cgccgatctc gcagtccttg acgtaggcgg cgcggaccac
    11821 ttcgttcatg gcattgcgca tgggaaccgg ttctcgggcg acgccgccgc gcccgtcggg
    11881 ctgctcgagc tcgaatgccg cgtcggcgca cacatgatcc gggtattgcg tggtctcgtc
    11941 cggccgtccc ttgatgccgg tcgcgaacgt gctcgccgcg ttcgacgaga cctccgaccc
    12001 gaacaggtcg agcgacgagc tgaaccagaa gttcagatag cgctgaagga gcggaaggtc
    12061 gatcgcgccg cggcgccgca ccgcgtcggg gtcgtccgag ccggcctcct tcatcgcctc
    12121 gagcgcgcgc ctcacgacgc ggccgatccc ggtctcgccg acgaacatgt ggtgcgcttc
    12181 ctcggtgagc atgaagcggc aggtgcgcgc gagcggatcg aacgcggact ccgcgaggct
    12241 cttcagctgg aacttgccgt cgcggtcggt gaaatacgtg aagcagaaga acgagagcca
    12301 gtcgtcgatc ggctcgttga acgtcccgag aatgcgcggc ttgtcgacgt cgcccgagtg
    12361 ccgcgcgagc agctcctcgg cctcttcgcg gccgtcgcgc ccgaaatagg cgtgcaggag
    12421 atacaccatc gcccagaggt ggcgcccttc ctcgacgttg acctggaaga ggttgcgcaa
    12481 gtcgtagagc gaagggcagg tcaatccgag cctgcgctgc tgctcgaccg aagcgggctc
    12541 ggtgtcgccc tgtgtgacga tgagccggcg gaactgcgag cggaattcgc ccgggacctg
    12601 ctgccacacg ggctgtccga gcgcgtcgcc gaagccgatg cggcggtccg ggatcgcgtc
    12661 ggcgaggaag atgccccagc ggtaatcggg catcttcacc gtgccgtagt gcgcccagcc
    12721 ctgcgcgtcg acgccgaccg cggtgcgcag gtacacgtct gcacccgcga agtcgctcgg
    12781 gcccatctcg cgccaccagt cgaggaagcg cggctgccag tgctcgagcg cgcgttgcag
    12841 cgcgcggtcg tgggcgaggt cgacgttgtt gggaatcttc tccgagtagt cgatcgcggt
    12901 catacgcgtt cccggttgaa tttcgccttg gcgcccgtgc cgtagacttt cagcgcgccg
    12961 tgatccccga cggcgttcgg ccggttgaag atccagttct gccacgccga gagccggccg
    13021 aagatgcggc tcgccatggt ctccttgccg gtgaagcgca ggttcgcctc caggccggtg
    13081 agcgcgtcgg gcgacaggct cgcgcgctcc tcgatcgcga tgcgcacctc gtcgtcccag
    13141 tcgagatcgt cgggcgtgaa ggtgaccagc cccagctcgc gcgcgaggcg cgcatcgagc
    13201 ttgtggccga tgcg
//
LOCUS       DATDMW010000218         3500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_936640, whole genome shotgun sequence.
ACCESSION   DATDMW010000218 DATDMW010000000
VERSION     DATDMW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936640"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VI319_09550"
     CDS             complement(<1..788)
                     /locus_tag="VI319_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY6864133.1"
                     /translation="MKLFMRLAAGLAAVVAASAFAQNVSIATGGTGGVYYPLGGGLAA
                     ALSKYVPGMQATAEVTGGSVDNLKLVGSGKPYVAFSMADAAQDAYRGEDKFKGNKVPV
                     RTLAVLYPNRMHVVTVEGMGIEKLTDLKGKRVSTGSPGSATEVMAFRIIEAAGLDKDR
                     DMKRERLGVAESVNAIKDHKIDAFFWVGGLPTAAVTDLANTPGVKLKMIDHAYVVKRM
                     NQKYGDLYVEDVIPKSTYRGMDADNKQATVMNILVAHANMPDQEA"
     gene            complement(876..1838)
                     /locus_tag="VI319_09555"
     CDS             complement(876..1838)
                     /locus_tag="VI319_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6864134.1"
                     /translation="MRIVATLVLMWAASVASAQTYPARPVTMIGPFAAGGPVDTIARL
                     LAVPMSKALGQPVLVEAVTGAAGTIGVGRVAHAAPDGYTLSIGHWSTHVINGAIYPLQ
                     YDLLRDFAPIAMICANPMLVVAGPSLPVSDLRALIDWLKANPGKGSLGTAGVGSGSHM
                     GGVYFQRATHTDFQYIPYRGTGPAMQDLVAGHIQFMVDQASNSLPQVRGGKIRAFAVT
                     AKARLSAAPQIPTVDEVGLPGLYMSVWYGIWAPRGTPREVVERLNAAVVASLADPTVR
                     QRLGELGQEIPPREQQTPEALGELQKAEIAKWWPIVKAAGIKPE"
     gene            complement(1852..2454)
                     /locus_tag="VI319_09560"
     CDS             complement(1852..2454)
                     /locus_tag="VI319_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6864135.1"
                     /translation="MKHTGRAAALSVVFAAALFSLAAVPVARADADAGRQKAAPCAAC
                     HGPEGNSANPAVPSLAGQPAQFITMQLFQFREGNRKNEQMTPMAANLSNADMNDLAAY
                     FSALKPAPPARQASAEHAAAAPALTKKYNCVQCHGPALVGQQHIPRLAGQQLDYLRAQ
                     LRGFKAQTRADIDGIMTSAAQPLTEADIEALADYLSALPR"
     gene            complement(2451..>3500)
                     /locus_tag="VI319_09565"
     CDS             complement(2451..>3500)
                     /locus_tag="VI319_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY6864136.1"
                     /translation="PLDKLKVPAGFKVEVWAEGIPEARSLALGDKGTVFVSNRLSKNV
                     YAVVDRNGRREVKTVLKGLDAPNGIAFSKGTLYVAERGRITRYDGIEANLDNPPEAKV
                     VIDGLDPTRQAGHFWKYLVMGPDGKLYFNIGAPGNIVMPSYMQASVMRVDPKSGVLET
                     VALGVRNSVGMDFDPKSKDLWFTNHGRDWLSDDTPNDTLHRVAVKNKIIPNFGYPFCH
                     QGDIVDPEFGKNRSCAEFAKPVAKLGAHIAPLGVKFYSGKMFPAEYRNNMFIAMHGSW
                     NRTTKQGYNVMRATVGENGKVKLQPFLTGFLTDEKADPPMWGRPVDLLQMRDGSLLVS
                     DDYNGVLYRISYAKK"
BASE COUNT          487 a         1188 c         1218 g          607 t
ORIGIN      
        1 gcctcctggt cgggcatgtt cgcgtgcgcg accaggatgt tcatgaccgt ggcctgcttg
       61 ttgtcggcgt ccatgccgcg gtacgtgctc ttcgggatca cgtcctcgac gtagaggtcg
      121 ccgtacttct ggttcatcct cttcaccacg taggcgtggt cgatcatctt gagcttgacg
      181 cccggcgtgt tggcgaggtc ggtgaccgcc gccgtcggga ggcccccgac ccagaagaag
      241 gcgtcgatct tgtggtcctt gatcgcgttt accgactcgg cgacgccgag gcgttcgcgc
      301 ttcatgtcgc ggtccttgtc caggccggcg gcctcgatga tgcggaacgc catcacctcg
      361 gtcgcgcttc ccggcgagcc ggtcgacacg cgcttgccct tgaggtctgt cagcttctcg
      421 atgcccatcc cctcgaccgt gacgacgtgc atgcggttcg ggtacagcac ggcgagcgtg
      481 cgcaccggca ccttgttgcc cttgaacttg tcctcgccgc ggtaggcgtc ctgcgccgcg
      541 tccgccatcg agaacgcgac atagggcttg ccgctcccga ccagcttgag gttgtcgacc
      601 gagccgccgg tcacctcggc ggtggcctgc atcccgggca cgtatttcga gagcgcggcg
      661 gcgagaccgc cgccgagcgg atagtagacg ccgcccgtgc cgccggtggc gatggaaacg
      721 ttctgcgcaa aggcgcttgc ggcgacaacg gcggccagcc ccgccgcgag gcgcatgaag
      781 agcttcatca ttcctccttc gatcgtgaga agacggggcc cactcctgcc ccggacgccg
      841 ctctgaaggc ggcgatccat tctagcctca acgcgtcatt cgggcttgat ccccgcggcc
      901 ttgacgatcg gccaccactt ggcgatctcc gccttctgca gctcgccgag cgcctcgggc
      961 gtctgctgct cgcgcggcgg gatctcctgc ccgagctcgc ccagccgctg gcgcaccgtc
     1021 gggtcggcga gcgacgcgac gaccgcggcg ttgagccgct cgacgacctc ccgcggcgtg
     1081 ccgcgcggcg cccagatccc gtaccacacc gacatgtaca ggccgggcag gccgacctcg
     1141 tcgacggtgg ggatctgcgg cgcggcggag agccgcgcct tcgcggtcac cgcgaacgcc
     1201 cggatcttcc cgccgcgcac ctgcggcagg gagttcgagg cctggtcgac catgaactgg
     1261 atgtgcccgg cgacgagatc ctgcatcgcc ggcccggtgc cgcggtacgg gatgtactgg
     1321 aaatcggtgt gcgtcgcgcg ctggaagtag acgccgccca tgtggctccc cgagcccacg
     1381 cccgccgtgc cgagcgagcc cttgcccggg ttagccttca gccagtcgat cagcgcccgg
     1441 agatcggaca ccggcagcga cgggccggcg acgaccagca tcgggttggc gcagatcatc
     1501 gcgatcggag cgaaatcccg gagcagatcg tattgcagcg gatagatcgc gccgttgatg
     1561 acgtgcgtgc tccagtgacc gatcgagagc gtgtatccgt cgggcgccgc gtgcgcgacg
     1621 cggccgacgc cgatcgtccc cgccgcgccg gtcacggcct cgaccagcac gggctgtccg
     1681 agcgccttcg acatggggac ggcgaggaga cgcgcgatcg tgtccaccgg cccgcccgcg
     1741 gcgaacggcc cgatcatcgt caccggtcgc gcgggatagg tctgcgcgga agcgaccgag
     1801 gcggcccaca tcaggaccag cgtggcgacg atccgcatgg gcgctcctct cctagcgcgg
     1861 caacgccgag aggtaatcgg cgagcgcctc gatgtcggcc tcggtgagcg gctgcgccgc
     1921 cgacgtcatg atgccgtcga tgtcggcgcg cgtctgcgcc ttgaagccgc gcagctgcgc
     1981 gcgcaggtag tcgagctgct gcccggcgag ccgcgggatg tgctgctgtc ccacgagcgc
     2041 cgggccgtgg cactgcacgc agttgtactt cttcgtgagc gcgggcgccg cggccgcgtg
     2101 ctcggcgctc gcctggcgcg cgggcggcgc gggcttcagc gccgagaaat acgccgcgag
     2161 gtcgttcatg tcggcgttcg agaggttcgc cgccatcggc gtcatctgct cgttcttgcg
     2221 gttgccctcg cggaactgga acagctgcat cgtgatgaac tgcgccggct gcccggcgag
     2281 cgagggcacc gcgggattcg ccgagttgcc ctccggcccg tggcaggcgg cacagggcgc
     2341 ggccttctgg cggcccgcgt cggcgtcggc gcgcgcgacc gggacggccg cgagagagaa
     2401 aagcgcggcc gcgaagacga ctgaaagcgc ggccgcgcgc ccggtgtgct tcacttcttc
     2461 gcgtagctga tgcggtacag gaccccgttg tagtcgtcgg agacgagcag cgagccgtcg
     2521 cgcatctgca ggaggtcgac cggccggccc cacatcggcg ggtcggcctt ctcgtcggtc
     2581 aggaagccgg tgaggaaggg ctggagcttc accttgccgt tctcgcccac cgtcgcccgc
     2641 atcacgttgt agccctgctt ggtcgtgcgg ttccacgagc cgtgcatcgc gatgaacatg
     2701 ttgttgcggt actcggccgg gaacatcttg ccgctgtaga acttcacgcc gagcggcgcg
     2761 atgtgcgcgc cgagcttggc gacgggcttg gcgaactcgg cgcaggagcg gttctttccg
     2821 aactcggggt cgacgatgtc gccctggtgg cagaacgggt agccgaagtt cgggatgatc
     2881 ttgttcttca ccgccacccg gtgcagcgtg tcgttcggcg tgtcgtcgct cagccagtcg
     2941 cggccgtggt tggtgaacca caggtccttg ctcttcggat cgaagtccat tccgaccgag
     3001 ttgcgcacgc cgagcgcgac cgtctcgagc acgccgctct tcggatcgac gcgcatgacc
     3061 gaggcctgca tgtagctcgg catcacgatg ttgccgggcg cgccgatgtt gaagtagagc
     3121 ttgccgtcgg gacccatcac caggtatttc cagaagtggc ctgcctggcg ggtgggatcg
     3181 aggccgtcga tcacgacttt cgcctcgggc ggattgtcga ggttcgcctc gatgccgtcg
     3241 tagcgcgtga tgcggccgcg ctcggcgacg tagagggtgc ccttgctgaa ggcgatgccg
     3301 ttcggcgcgt cgagcccctt cagcacggtc tttacctcgc gccggccgtt gcggtcgacc
     3361 acggcgtaca cgttcttcga gaggcggttg ctgacgaaca ccgtgccctt gtcgccgagg
     3421 gcgagcgagc gcgcctcggg gatgccctcg gcccagacct cgaccttgaa gccggccggg
     3481 actttcagct tgtcgagcgg
//
LOCUS       DATDMW010000219         2553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2184285, whole genome shotgun sequence.
ACCESSION   DATDMW010000219 DATDMW010000000
VERSION     DATDMW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184285"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VI319_09570"
     CDS             complement(<1..702)
                     /locus_tag="VI319_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864137.1"
                     /translation="MSLSLDIAQPSPVAPGRSADRLAGKIRLAFIMSATHSGSTLLAM
                     QLARQQGVCSVGELSGTRHRARPGYRCSCGAELVDCDFWRRVSAAMARRGFSFSATTA
                     ETDVRNAPSPYARRLLRPMHRGPFLELVRDVALQVSPAARDYLRRHVQLKTALAESVL
                     ECSGDSVLVDSSKLGVQLKYHLRNPRLDVKVLWLVRDGRAVSCSLMRTQKATMSEAAY
                     EWRRFYEEADAIVRRL"
     gene            complement(717..>2553)
                     /locus_tag="VI319_09575"
     CDS             complement(717..>2553)
                     /locus_tag="VI319_09575"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEY6864138.1"
                     /translation="VPEPDLSRLGAKWIFPNENIDAAPYVTAAERAAMGRLDVFALAD
                     LDLGLPPRWNRDPKTGIEAPLRFGKTLDYRDPDLVGDIKYLWEPNRHLQLVTLAQAYA
                     LTRRRKYFDALVEQLDSWFLACPYGRGANWSSALEAGIRLVNWSAAWHLVGGHEALDA
                     DVRARWLKSVYQHARFVRHWFSSHSSANNHLLGEATGLYVAALTWPHWPEAADWLLTS
                     RKILEREALRQVAPDGVSREQATWYQQFVLDMLLVALFAGRANGERFSPQFESRLEAM
                     IDFIGCIMDAGGNVPMFGDADDGALLRLAPGAASPFRSALGLGALLFRRGDFKLKAGA
                     IDDKARWLFGEEADRAFGALEPETTRLPLRQAFPDGGYYILGCGFDTPREVRAVVDAG
                     PLGYTAIAAHGHADALSFTLSVHGREFLIDPGTYAYHTQPAWRGYFRGTSAHNTLRVD
                     GVDQSEQGGNFLWLRKARAGCSLWLSTPEADSFEGWQDGYLRLADPVKHRRLVSFDKR
                     LRRLVVEDRLEMQGSHDLELFFHCAEACSVDAAPGGFVLSRDGIAVRIALPPSGDSSL
                     HRGELAPILGWVSRAFDRRAPTHTIAWRARLSGTALLRTEIQIAS"
BASE COUNT          382 a          876 c          949 g          346 t
ORIGIN      
        1 gaggcggcgc acgatggcgt cggcctcttc gtagaagcgc cgccattcgt aggcggcctc
       61 cgacatggtc gccttctgcg tgcgcatcag gctgcaggag acggcgcgcc cgtcgcgcac
      121 cagccagagc accttcacgt cgaggcgcgg gttgcgcagg tggtacttga gctgcacgcc
      181 gagcttcgag gagtcgacga gcaccgagtc gccgctgcat tccagcacgc tctccgcgag
      241 cgcggtcttg agctgcacgt ggcgccgcag gtaatcgcgc gccgcgggcg agacctgcag
      301 cgcgacgtcg cggaccagct cgaggaacgg gccgcgatgc atcggccgca gcagccgccg
      361 cgcatacggg ctcggcgcgt tgcgcacgtc ggtctcggcc gtggtcgcgc tgaaggaaaa
      421 gccgcgccgc gccatcgccg cggagacgcg gcgccagaag tcgcagtcga cgagctcggc
      481 gccgcacgag caccggtacc ccggccgcgc gcggtggcgc gtgccgctca gctcgccgac
      541 gctgcacacg ccctgctgcc gcgcgagctg catggcgagc agcgtcgagc cggagtgggt
      601 ggcggacatg atgaacgcga ggcggatctt gccggcgagg cggtcggccg agcgcccggg
      661 agcgacgggc gagggctgcg cgatatcgag ggagaggctc atctggtttt tccttttcag
      721 cttgctatct ggatctcggt gcgcagcagc gccgtgccgg acaggcgcgc gcgccaggcg
      781 atggtgtgcg tcggcgcgcg ccgatcgaac gcgcgcgaca cccagccgag gatcggcgcg
      841 agctcgcccc ggtgcagcga cgaatcgccg ctcggcggca gcgcgatccg cacggcgatg
      901 ccgtcgcgcg agagcacgaa gccgccgggc gccgcgtcga cgctgcacgc ctcggcgcag
      961 tggaagaaga gctcgaggtc gtgcgagccc tgcatctcga ggcggtcctc gacgacgagg
     1021 cggcgcagcc gcttgtcgaa cgagacgagc cggcggtgct tcaccgggtc ggcgaggcgc
     1081 aggtaaccgt cctgccagcc ttcgaagctg tccgcctccg gcgtggagag ccacaggctg
     1141 cagccggcgc gcgccttgcg cagccacagg aagttgccgc cctgctccga ctggtcgacg
     1201 ccgtcgacgc gcagcgtgtt gtgcgccgac gtgccgcgga agtatccgcg ccacgccggc
     1261 tgcgtgtgat aggcgtaggt gcccggatcg atgaggaact cgcggccgtg cacggagagc
     1321 gtgaaggaga gcgcgtccgc gtggccgtgc gcggcgatcg cggtgtagcc gagcggcccc
     1381 gcgtcgacca cggcgcgcac ctcgcgcggc gtgtcgaagc cgcagccgag gatgtagtag
     1441 ccgccgtcgg ggaacgcctg gcgcagcggc agccgcgtcg tctcgggctc gagggcgccg
     1501 aacgcccgat cggcctcctc gccgaagagc cagcgcgcct tgtcgtcgat cgccccggcc
     1561 ttcagcttaa agtcgccgcg ccggaagagc agcgcgccga ggccgagcgc cgagcggaag
     1621 ggcgacgccg cgccgggcgc gagccgcagc agcgcgccgt cgtcggcgtc gccgaacatc
     1681 ggcacgttgc cgcccgcgtc catgatgcag ccgatgaaat cgatcatcgc ctcgaggcgc
     1741 gactcgaact gcggcgagaa gcgctcgccg ttcgctctcc cggcgaagag cgccaccagc
     1801 agcatgtcga gcacgaactg ctggtaccac gtcgcctgct cgcggctcac gccgtcgggg
     1861 gcgacctgcc gcaacgcctc tctttccagg atcttcctgc tggtgaggag ccaatccgcg
     1921 gcttcgggcc agtgcggcca ggtgagcgcg gcgacgtaga gcccggtcgc ctcgcccagc
     1981 aggtggttgt tcgccgagga atgcgacgag aaccagtggc gcacgaagcg cgcgtgctgg
     2041 tacaccgact tcagccagcg cgcgcgcaca tccgcgtcga gggcttcgtg acctcctacg
     2101 aggtgccacg cggccgacca gttgacgagg cggatgccgg cttcgagcgc gctcgaccag
     2161 ttggcgcccc ggccgtacgg acaggcgagg aaccagctgt cgagctgctc gacgagcgcg
     2221 tcgaagtact tgcgccggcg cgtgagcgcg tacgcctgcg cgagcgtgac gagctgcagg
     2281 tggcggttcg gctcccagag atacttgatg tcgccgacga ggtcggggtc gcggtagtcg
     2341 agcgtcttgc cgaagcgcag cggcgcctcg atgccggtct tcggatcgcg gttccagcgc
     2401 ggcggcaggc cgagatcgag gtcggcgagc gcgaatacgt cgagccggcc catcgccgcg
     2461 cgctcggccg cggtcacgta gggcgcggcg tcgatgttct cgttaggaaa aatccatttc
     2521 gcgcccaggc gcgacaggtc gggctcgggc acc
//
LOCUS       DATDMW010000220         3923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1686293, whole genome shotgun sequence.
ACCESSION   DATDMW010000220 DATDMW010000000
VERSION     DATDMW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686293"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VI319_09580"
     CDS             <1..402
                     /locus_tag="VI319_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864139.1"
                     /translation="WRANADWMVTPKTALGFETYYEPRSVIAVGVSDALVRGVAFGPG
                     WAPTAKLNFSARVMRERLDYHGGSFTFSVPGVTPPTLELVRLYRLGAYWEYTRQIHWQ
                     FALEHGERESNVLGRDYTYNAIIANVRYLFW"
     gene            complement(445..1890)
                     /locus_tag="VI319_09585"
     CDS             complement(445..1890)
                     /locus_tag="VI319_09585"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY6864140.1"
                     /translation="MIQPVILCGGSGTRLWPLSREYHPKPLHAVNGERTLLQQTAARC
                     IDVAAITDPLVICNEAHRFMVQAQLARAGSRPRAILLEPKGRGTAPALTLAAVHSVTA
                     DDPVLLAMPSDHSIADAAAFRRAVERGAALAQQGFIVTFGVPPRSPETGYGYIRAGRA
                     LGGSSGEEAAFIEAFVEKPDAQRAQEYFASGRFLWNSGIFAVRASVWLEAIAAFRKDI
                     FAACERACRQGRRDGAFLRVDAPAFDACPADSIDYAVMERIVGEAADASGSLHAVVVR
                     LDAGWSDVGAWDALWSIEQKDAEGNVIHGDVHAADTKNALLISQHRLLACVGLEDVVV
                     VETPDAVMVAKKDKAQKVGELVARLKAAGRDETLVHRKVHRPGGSYDGIERGERFQVK
                     RIVVDPGAALSLQMHYHRAEHWIVVRGTARVTRGEESFLLTENESTYIPPGTRHRLEN
                     PGRLPLEIIEVQSGAYLGEDDIVRFDDAYGR"
     gene            complement(1945..2421)
                     /locus_tag="VI319_09590"
     CDS             complement(1945..2421)
                     /locus_tag="VI319_09590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6864141.1"
                     /translation="MGMNRSGGLYVGELAPIDGSAVLVEALSLFPGARIQALPSGEAH
                     AAQRARAMERSAYLVVAPHDGDLQRTLAEAFAQGLAVIAPHEGACAELVEPGRNGLHY
                     EPGSPRDLARRLAWAEAFPERMLQMGECARADYLARFISHWSYPKLFGERRRSARV"
     gene            complement(2408..2722)
                     /locus_tag="VI319_09595"
     CDS             complement(2408..2722)
                     /locus_tag="VI319_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864142.1"
                     /translation="MSAAATAAGYLYASGRLRGRGAREQEHGTEDPKRRTQETGPDAL
                     SHERAALRKPLRKPVRVAALPPEEPASAGRPKRAPRVQYRVRRWRPEQEAGEGREPHG
                     HE"
     gene            complement(2749..3552)
                     /locus_tag="VI319_09600"
     CDS             complement(2749..3552)
                     /locus_tag="VI319_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864143.1"
                     /translation="MNLSFGLLKKAADPQADRLLAREARWLRELEDVRELEGHVPRLL
                     EEGVTSPEGRRFLVISMVPPRGETRAFTDDHARFLASLGKARFRAIDFEVSGTCQWLL
                     RSLTELEDFAPPATVSALGDAYHDCETALLYWTGPYVLAQGDFAPWNIRTLAPGLFVT
                     DWGNARAEASPLDDVLHFLMIQRAACRRPISVPGLQSAMKRAGEFALRAYPEWTWRPR
                     VIGALTLVYLLGTLLHRVLAARRVELADPVVGAYWKLLEKRSAWMPKQD"
     gene            complement(3549..3872)
                     /locus_tag="VI319_09605"
     CDS             complement(3549..3872)
                     /locus_tag="VI319_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864144.1"
                     /translation="MQTSLRQKRLREQRSAFLAFMVLALIYLLAGLPIVYRSLAAQAA
                     HAAPYGMPGFATLDRNHDGYVDSSETALWPAYAGAFARADLNGDGRLDAAEFRAGLKS
                     LARAR"
BASE COUNT          557 a         1388 c         1395 g          583 t
ORIGIN      
        1 tggcgcgcga acgcggactg gatggtcacg cccaagaccg cgctcggctt cgagacctac
       61 tacgagccgc gctcggtcat cgccgtcggg gtgagcgacg cgctcgtgcg cggcgtcgcc
      121 ttcggccctg gctgggcgcc gaccgcgaag ctcaacttct cggcgagggt catgcgcgag
      181 cggctcgact accacggcgg ctcgttcacc ttctcggttc cgggcgtaac gccgcccacg
      241 ctcgagctcg tgcggctcta tcgcctcggc gcctactggg agtacacgcg gcagatccac
      301 tggcagttcg ccctcgagca cggcgagcgc gaatcgaacg tcctgggacg cgattacacg
      361 tacaacgcga tcatcgccaa cgtgcgctac ctgttctggt agcgaacccg ccgctcgcct
      421 gcggcttacg gcccggcgag ctggctagcg gccgtaggcg tcgtcaaagc gcacgatgtc
      481 gtcttcgccg aggtaggcgc ccgactgcac ctcgatgatc tcgagcggca ggcggcccgg
      541 attctccagc cgatgccggg tgcccggcgg gatgtaggtc gactcgttct cggtgaggag
      601 aaacgattcc tcgccgcgcg tgacgcgcgc ggtgccgcgc acgacgatcc agtgctcggc
      661 gcggtggtaa tgcatctgca gcgaaagcgc ggcgccgggg tcgacgacga tgcgcttcac
      721 ctggaagcgc tcgccgcgct cgatgccgtc gtagctgccc ccgggccggt gcaccttgcg
      781 gtgcacgagc gtctcgtcgc gccccgcggc cttcaggcgc gcgaccagct cgccgacctt
      841 ctgcgccttg tccttcttcg cgaccatcac cgcatcgggc gtctcgacca ccaccacgtc
      901 ctcgaggccg acgcacgcga gcaggcggtg ctgcgagatg agcagcgcgt tcttcgtgtc
      961 ggcggcgtgc acgtcgccgt ggatgacgtt gccctcggcg tccttctgct cgatcgacca
     1021 gagcgcgtcc caggcgccga cgtcggacca gcccgcgtcg agccgcacca ccaccgcgtg
     1081 caggctgccg gaggcgtcgg cggcttcacc gacgatccgc tccatcacgg cgtagtcgat
     1141 ggaatcggcc gggcaggcgt cgaacgcggg cgcgtcgacg cgcaggaacg cgccgtcgcg
     1201 ccgcccctgc cggcaggcgc gctcgcacgc ggcgaagatg tccttgcgga aagccgcgat
     1261 cgcttcgagc cagaccgagg cgcgcacggc gaagatgccg ctgttccaga gaaagcggcc
     1321 ggaggcgaag tattcctgcg ctctttgcgc atcgggcttc tcgacgaacg cttcgatgaa
     1381 ggccgcctcc tccccggacg acccgccgag cgcgcgaccg gcgcggatat agccgtagcc
     1441 ggtctccggc gagcgcggcg gaacgccgaa agtgacgatg aagccctgct gcgcgagcgc
     1501 cgcgccgcgc tccaccgcgc gccggaacgc cgccgcgtcg gcaatgctgt ggtccgaggg
     1561 catggcgagc agcaccgggt cgtcggccgt gacgctgtgc accgcggcga gggtgagcgc
     1621 cggcgcggtg ccgcggccct tcggctcgag gaggatggcg cgcggccggc tgccggcgcg
     1681 cgcgagctgc gcctgcacca tgaagcggtg cgcctcgttg cagatcacca gcggatcggt
     1741 gatcgccgcg acgtcgatgc agcgcgccgc cgtctgctgc agcagcgtgc gctcgccgtt
     1801 gaccgcgtgc aggggcttcg gatggtattc gcgcgaaagc ggccagagcc gcgtgcccga
     1861 cccgccgcag agaatgaccg gctggatcat ggtcggcgtg acgccgagct cgatcacctg
     1921 gactcctcga gttgcgaagc tgctctaaac gcgggcgcta cggcgccgct ccccgaacag
     1981 tttcggatag ctccaatgcg agataaaccg cgcgagatag tccgctcggg cgcactcgcc
     2041 catctgcagc attcgttcgg ggaaggcttc cgcccaggcg aggcggcgtg cgagatcgcg
     2101 cggcgagccc ggctcgtagt gcagcccgtt gcggccgggc tcgacgagct ccgcgcaggc
     2161 tccttcgtgc ggcgcgatca ccgccagtcc ctgcgcgaac gcttctgcga gcgtgcgctg
     2221 cagatcgccg tcgtggggcg cgacgacgag gtacgccgag cgctccatcg ctcgcgcgcg
     2281 ttgcgcggca tgggcttcgc cggagggaag cgcctggatg cgggcgccgg gaaagagcga
     2341 gagcgcctcg acgagcaccg cggagccgtc gatcggcgcg agctcgccga cgtacaggcc
     2401 gcccgatcta ttcatgccca tgcggctcgc ggccctcgcc ggcttcctgc tccggccgcc
     2461 agcggcgtac gcgatattgc acgcgcgggg cgcgcttcgg ccgcccggca ctcgccggct
     2521 cctcgggcgg cagcgcggcg acgcgaaccg gtttacgcag cggcttcctg agcgccgcgc
     2581 gctcgtgcga gagcgcgtcg ggccccgttt cctgcgtgcg gcgcttcgga tcttccgtgc
     2641 cgtgctcctg ctcgcgcgcg ccgcggccgc gcaggcggcc cgaggcgtag aggtatccgg
     2701 cggcggtggc ggcggcgctc atggctttct ctgttctcct tgcattcttc agtcctgctt
     2761 cggcatccac gccgagcgct tctcgagcag cttccaatag gcgccgacga ccgggtcggc
     2821 cagctcgaca cgccgcgcgg ccagcacgcg gtgcagcagc gtgcccagca gatacacgag
     2881 cgtgagcgcg ccgatcaccc tcggccgcca cgtccattcc gggtaggcgc gcaatgcgaa
     2941 ctcgccggcg cgcttcatcg ccgactgcag gcccggcacg ctgatcggcc ggcggcacgc
     3001 cgcgcgctgg atcatgagga aatgcagcac gtcgtcgagc gggctcgcct cggcgcgcgc
     3061 gttgccccag tcggtgacga aaagccccgg ggcgagcgtg cggatgttcc agggcgcgaa
     3121 atcgccctgc gcgagcacgt acggcccggt ccagtagagg agcgccgtct cgcagtcgtg
     3181 gtacgcatcg ccgagcgccg acacggtcgc gggcggagca aagtcctcga gctcggtcag
     3241 cgagcgcagc agccactggc aggtcccgga cacctcgaag tcgatcgcgc ggaagcgcgc
     3301 cttgccgagg ctcgcgagga agcgcgcatg atcgtcggtg aacgcgcgcg tttcgccgcg
     3361 cggcggcacc atggagatga cgaggaagcg ccggccttcc gggctggtca cgccttcctc
     3421 caggagccgc ggcacgtggc cttcgagctc gcgaacgtcc tcgagctcgc gcagccagcg
     3481 cgcctcgcgc gcaagcagcc ggtcggcctg cggatcggcg gctttcttga gcaggccgaa
     3541 ggaaaggttc atcgcgccct cgccaggctc ttcagccccg cgcggaactc cgcggcatcg
     3601 aggcggccgt cgccgttcag gtcggcgcgg gcaaaggcgc ccgcgtaggc gggccagagc
     3661 gccgtttccg agctgtcgac gtagccgtcg tggttgcggt cgagggtcgc gaaccccggc
     3721 atgccgtagg gcgcggcgtg cgcggcttgc gcggcgaggc tgcggtagac gatcggcagg
     3781 cccgcgagca gatagatcag cgcgagcacc atgaaagcca gaaaggcgct tcgctgctcg
     3841 cgcagccgct tctgccgcag ggaagtctgc atcttggcgt tatttcgtgt cggactgtgg
     3901 cgtgccccgt gcaagcggcg tac
//
LOCUS       DATDMW010000221         6740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_69417, whole genome shotgun sequence.
ACCESSION   DATDMW010000221 DATDMW010000000
VERSION     DATDMW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69417"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1598
                     /locus_tag="VI319_09610"
     CDS             192..1598
                     /locus_tag="VI319_09610"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY6864145.1"
                     /translation="MSDPCELGAAALVERYRDGSLSPVEATRAILRRIERLNPVLNAF
                     CLVDAKSALAAAAASEARWRSNEPLGALDGVPVSVKDVILARGWPTRRGSKTVDPKGP
                     WKDDAPAVARLREAGAVLLGKTTTPEFGWKGVTDSPLTGITRNPWDPKKTPGGSSGGA
                     AAAVASGMGALALGTDGGGSIRIPSAFTGVFGIKPTHGRVPFWPPSPTGTVAHMGPIA
                     RSVTDAARMLAVLALPDARDWAASPRERRDWLATLEDGVRGLRIAYSADLGYARVDAE
                     IARIARAAVRTFERLDAIVEERDPGFDDPHDLFTRHWFPGAAWIVRSAPPGKRRLMDK
                     GLLAVAKLGERYSAVDYVDAAVQRAALGARMGAFHERYDLLVTPTMPIAAFDAGREVP
                     PKWKTKRWTDWTPFTFPFNLTQQPAASIPCGRTAAGLPVALQIVGARHADALVLRAAR
                     AFEREHPIPLPDVSRLAK"
     gene            complement(1640..2026)
                     /locus_tag="VI319_09615"
     CDS             complement(1640..2026)
                     /locus_tag="VI319_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864146.1"
                     /translation="MPAKKILIVDDSATDRQFYVDTLTRAGYDVVTAQNGEEGVYKSR
                     IEQPDLILMDIVMPGMDGYKATRTVVRDEATKHIPVIICTSRNQETDRLWGMRQGARD
                     FLMKPIREKDLVAKVAAATQPASKVA"
     gene            complement(2110..2346)
                     /locus_tag="VI319_09620"
     CDS             complement(2110..2346)
                     /locus_tag="VI319_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864147.1"
                     /translation="MDKILIVVGSLMLAFSSFAVEPAKPVKISMATETAQPQVHVAKA
                     VKEPKAKAPKKWVDEGDSRLNDYRLERESCCGPQ"
     gene            complement(2511..3293)
                     /locus_tag="VI319_09625"
     CDS             complement(2511..3293)
                     /locus_tag="VI319_09625"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY6864148.1"
                     /translation="MTADAIPLEKNRSVRERVSEAEWRLRCELAAAHRLVAHFVFVDL
                     TYNHISARVPGEPRHFLVKADNVFMEQVTASNLVKYDVDGRQVMESAWKASPAAYNLH
                     AAVLKTRPDIMAAVHTHSPANLAVSAQRRGLLPITQQAMRFYDRIAYYPNEVDDTTPE
                     GTAALARALGDRWTMILENHGALVCGSSLAEAYIYHHFFELACRAQVGALAGGGELIT
                     PAPGVCEERAKKFGRIGQYDSSSRDWVAGMALVRRLYPDFDS"
     gene            3317..4285
                     /locus_tag="VI319_09630"
     CDS             3317..4285
                     /locus_tag="VI319_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864149.1"
                     /translation="MPFPNKSRPNLAALLLFAAANVWGQSWPAHPIKMVVPNAPGGLS
                     DITARLLAPGLGERLGQQIVIENRPGAGGTIATAAVAKAPADGYTLVAVFDAHASNPH
                     LFHNLDYDGIADFAPVSLLVRGPLVLVVHPRVGAKSASELAALARARPGAINFAAVGP
                     GSPARLLAEYFKLAAGIDFTIVPYKGAAPALTDLVSGEVDAMFATVPSVSSHLKAGRL
                     RALAVTSEQPTPAVPGVPTMNEAYPGYVAEAWVGLLAPAKTPAAIVERIHDDAVQALA
                     DPALRARFAAQGLETVASTPAELERWIRVETERWGRVIREQKIKIE"
     gene            4300..5283
                     /locus_tag="VI319_09635"
     CDS             4300..5283
                     /locus_tag="VI319_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864150.1"
                     /translation="MAFSGLKALAAAAALVVAQGAAAQPAPAAAPYPTRTIRLYVGSP
                     PGSGSDLVARLMAQHFSERLGQPVVVEQKLGAAGLVAADAVAKALPDGHTLALISGAH
                     AANAAMRSSLPYDPVKDFAMIGTVVAYPMVVSVSAASPISSFPDLIRRARAAPGKLTY
                     AMTTGTLVHLLGEWINIEAGTNIVGVPYKGSALGLTDVLAGRVDAAIETATAAFGSVR
                     SGKLRPLAISSATRHPALPDLPTIAETLPGVEMTSWLGFVGPAGTPRPVVDRLNAETR
                     AVVALPEVQRKLADLSGVPTPSSPEEMRALVERDIARWTRVVELKKIPKQN"
     gene            complement(5333..6208)
                     /locus_tag="VI319_09640"
     CDS             complement(5333..6208)
                     /locus_tag="VI319_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HEY6864151.1"
                     /translation="MTRSLCALLCVLAAALPVASRGQDPQEEIRAALEAAQKAQVAGP
                     SEVKLLDQAVLKLPAGHVYFPPAEAGRLLQAMGNRTGDDLLGAIFPDRFNWFIVMRQV
                     KSGYVKDDDARDWNSDELLQSLKKGTAASNQDRRSRHIPEIEVIGWVEPPAYDAGTHR
                     LVWSAQSRELGEPDGAERGVNYNTYALGRDGYISMNLVTGMNSIEAQKPVARQMLAAL
                     EYNPGKRYADFDSSTDRVAEFGLLALIGGVAAKKLGLLALIAAFAVKFAKVIGIAVLG
                     LAAVAAKLLRRRPPA"
     gene            complement(6300..6593)
                     /locus_tag="VI319_09645"
     CDS             complement(6300..6593)
                     /locus_tag="VI319_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY6864152.1"
                     /translation="MPAEACQHIKDIVEVVRPKTRQCAECVKTGSSWVQLRTCQTCGA
                     TLCCDSSPNRHASRHARASGHPVIASAEPGDHWLYCFADDAYGEYDPSMQSTA"
BASE COUNT          930 a         2417 c         2366 g         1027 t
ORIGIN      
        1 tgcgctgcgc cagatcagcg agggacgcga caagccgcag gagccgccgc ccgttgtcaa
       61 cttccgcgag cccgtgatcc acacggtcac gaacgtcggc gacatgccgc tctggaactt
      121 catcctggag ttcaagccgc agtctggaaa cagcgcttcg gcgcgatgaa ggagcgggac
      181 gcgcccggga aatgagcgac ccctgcgagc tcggcgccgc ggcgctcgtc gagcgctacc
      241 gcgacggctc gctctcgccg gtcgaggcga cgcgcgcgat cctccggcgc atcgagcgcc
      301 tgaaccccgt gctgaacgcg ttctgcctcg tcgacgcgaa gagcgcgctc gcggccgcgg
      361 ccgcgtcgga ggcgcgctgg cgcagcaacg agccgctcgg tgcgctcgac ggcgtcccgg
      421 tctcggtgaa ggacgtgatc ctcgcgcgcg gctggccgac gcggcgcggc tcgaagaccg
      481 tcgacccgaa gggcccctgg aaggatgacg cgcccgcggt cgcccggctg cgcgaggcgg
      541 gcgcggtgct cctcggcaag accaccacgc ccgagttcgg ctggaagggc gtgaccgaca
      601 gcccgctcac cggcatcacg cgcaacccct gggatccgaa gaagacgccc ggcggctcct
      661 cgggcggcgc cgccgcggcc gtcgcctcgg gcatgggcgc gctcgcgctc ggcaccgacg
      721 gcggcggctc gatccgcatc ccgagcgcgt tcaccggggt gttcggcatc aagccgaccc
      781 acggccgcgt gccgttctgg ccgccgtcgc cgaccggcac ggtcgcgcac atgggcccga
      841 tcgcgcgcag cgtgaccgac gcggctcgca tgctggcggt gctcgcgctc ccggacgcgc
      901 gcgactgggc cgcgagcccg cgcgagcggc gcgactggct ggcgacgctc gaggacggcg
      961 tgcgcgggct gcgcatcgcg tactcggccg atctcgggta cgcgcgcgtc gatgcggaga
     1021 tcgcgcggat cgcgcgcgcc gcggtccgca cgttcgagcg cctcgacgcg atcgtcgagg
     1081 agcgcgatcc cgggttcgac gatccgcacg atctcttcac gcgccactgg ttcccggggg
     1141 cggcgtggat cgtgcgcagc gcgcccccgg gcaagcgcag gctcatggac aagggtctgc
     1201 tcgcggtggc gaagctcggc gagcgctata gcgccgtgga ctacgtggac gcggcggtgc
     1261 agcgcgccgc gctcggcgcg cgcatgggcg cgttccacga gcgctacgat ctgctcgtca
     1321 cgccgaccat gccgatcgcc gctttcgacg cgggccgcga ggtcccgccg aagtggaaga
     1381 ccaagcgctg gaccgactgg acgccgttta cgtttccctt caacctcacg cagcagcccg
     1441 cggcttccat cccctgcgga cgcaccgcgg cgggactccc ggtcgccctg cagatcgtcg
     1501 gcgcgcgcca cgccgacgcg ctcgtgctgc gcgcggcgcg cgccttcgag cgcgagcacc
     1561 cgatcccgct gcccgacgtc tcgcggctcg ccaaatgaaa aggcccgcct cggggcgggc
     1621 cttccccgaa ggtgacgctc tacgcgacct tggacgccgg ctgtgtcgcg gccgcgacct
     1681 tcgcgaccag gtccttctcg cggatgggct tcatcaggaa gtcgcgcgcg ccctggcgca
     1741 tgccccagag gcggtcggtc tcctggttgc ggctggtgca gatgatcacc gggatgtgct
     1801 tggtcgcctc gtcgcgcacg acggtgcgcg tcgccttgta gccgtccatc cccggcatga
     1861 cgatgtccat gaggatgagg tcgggctgct cgatgcgcga tttgtacacg ccctcctcgc
     1921 cgttctgcgc ggtgacgacg tcgtagccgg cgcgggtgag ggtgtcgacg tagaactggc
     1981 gatcggtggc cgagtcgtcg acgatcagga ttttcttggc tggcatcttt tgctccttgg
     2041 gtcatggcgg aagcgatccg gtccgggact ctccccggac cggatcgctt tcgccgttgt
     2101 cgattccgct tactgcgggc cgcagcagct ctcgcgctcg aggcggtagt cgttgaggcg
     2161 cgaatcgccc tcgtcgaccc acttcttcgg cgccttcgcc ttcggctcct tcactgcctt
     2221 cgccacgtgc acctggggct gcgccgtctc ggtcgccatc gagatcttga ccggcttggc
     2281 cggctcgacc gcgaacgaag aaaacgcgag catcaggctg cccaccacga tcaggatctt
     2341 gtccattacc cgtccccccg ttgctgttgg caccggccct ccccggggac ggtgtgcctg
     2401 ttcatgagca aaaggggtgc cagcacgcgg gcggcgccac gtaggcgggc cgggtgactt
     2461 acttcacgga aagacggccg gaaccgctcc cggcttgtcg ggcgaccgac tcagctgtcg
     2521 aagtcgggat aaagccggcg cacgagcgcc atgccggcga cccagtcgcg gctcgaagag
     2581 tcgtactggc cgatgcggcc gaacttcttc gcccgctctt cgcagacccc gggcgccggc
     2641 gtgatcaatt ccccgccgcc cgcgagcgcg ccgacctgcg cgcggcacgc gagctcgaag
     2701 aagtgatgat agatataggc ctcggcgagc gacgagccgc acaccagcgc gccgtggttc
     2761 tcgaggatca tcgtccaccg gtccccgagc gcgcgcgcga gcgccgccgt gccctcgggc
     2821 gtcgtgtcgt cgacctcgtt gggatagtag gcgatgcggt cgtagaagcg catcgcctgc
     2881 tgcgtgatcg gcagcaggcc gcggcgctgc gccgagaccg cgaggttggc cggcgagtgc
     2941 gtgtgcaccg ctgccatgat gtcgggccgc gtcttgagca ccgcggcgtg caggttgtag
     3001 gcggccgggc tcgccttcca ggccgactcc atgacctgcc ggccgtcgac gtcgtacttg
     3061 accaggttgg aagcggtcac ctgctccatg aacacgttgt cggccttcac caggaagtgg
     3121 cgcggctcgc ccggcacgcg cgcggagatg tggttgtagg tgagatcgac gaacacgaag
     3181 tgcgcgacca ggcggtgcgc ggcggcgagc tcgcagcgca gccgccactc ggcctcgctc
     3241 acgcgctcgc gcacggagcg atttttctcc aggggaattg cgtcggccgt cattgccgct
     3301 ctatgctagc accccgatgc cgtttccgaa caaaagccgc ccgaacctgg ccgcgctgct
     3361 ccttttcgct gccgcgaatg tctggggcca gtcatggccc gcccacccga tcaagatggt
     3421 ggtgccgaac gcgcccggcg ggctctcgga catcacggcg cgcctgctcg cacccggcct
     3481 cggcgagcgc ctcggccagc agatcgtgat cgagaaccgc cccggcgccg gaggcacgat
     3541 cgcgaccgcg gccgtcgcga aggcgcccgc cgacggctac acgctcgtcg cggtgttcga
     3601 cgcgcatgcc tcgaacccgc atctgttcca caacctcgat tacgacggca tcgccgactt
     3661 cgcgccggtg tcgctgctcg tgcgcgggcc gctcgtgctg gtggtgcatc cgcgcgtcgg
     3721 cgcgaagagc gcgagcgagc tcgcggcgct cgcccgcgcg cgtccgggtg cgatcaattt
     3781 cgcggccgtc ggcccgggct cgcccgcgcg gctgctcgcg gagtacttca agctcgccgc
     3841 cggcatcgac ttcaccatcg tgccctacaa gggcgccgcg cccgcgctca ccgacctcgt
     3901 ctccggagag gtcgacgcga tgttcgcgac ggtgccgagc gtcagctcgc acctcaaggc
     3961 gggacggctg cgcgcgctcg cggtgacgtc ggagcagccg acgcccgcgg ttcccggcgt
     4021 gcccaccatg aacgaagcgt acccgggcta cgttgccgaa gcctgggtgg ggctgctcgc
     4081 gccggcgaag acgccggccg cgatcgtcga gcgcatccac gatgacgccg tgcaggcgct
     4141 cgccgatccc gccctgcgcg cgcgcttcgc ggcccagggc ctcgagaccg tcgccagcac
     4201 gccggccgag ctcgagcgct ggatccgcgt cgagaccgag cgctggggcc gcgtgatccg
     4261 cgagcagaag atcaagatcg aataggcgga ggatcgggaa tggcgttctc gggattgaaa
     4321 gctctcgccg cggcggcggc gctcgttgtc gcgcagggcg cagcggcgca acccgcgccg
     4381 gcggcggcgc cgtatccgac gcgcacgatc cggctctacg tgggatcgcc gccgggctcg
     4441 ggcagcgacc tcgtcgcgcg gctgatggcg cagcacttct cggagcgcct gggacagccg
     4501 gtcgtggtcg agcagaagct cggcgccgcg ggcctggtcg ccgcggacgc ggtcgccaag
     4561 gcgctgcccg acggacatac gctggcgctg atctcgggcg cgcacgcggc gaatgcggcg
     4621 atgcgctcgt cgctgcccta cgacccggtc aaggacttcg cgatgatcgg caccgtggtc
     4681 gcctatccga tggtcgtgtc cgtgtcggcc gcgtcgccga taagctcctt cccggacctg
     4741 atccggcgcg cgcgcgcggc gcccgggaag ctgacgtacg caatgaccac gggcacgctc
     4801 gtgcacctgc tcggcgaatg gatcaacatc gaggcgggaa cgaacatcgt cggcgttccc
     4861 tacaaaggca gcgcgctcgg actcaccgac gtgctcgccg gccgcgtcga cgcggcgatc
     4921 gagacggcga ccgccgcgtt cggcagcgtg cgctcgggga agctccgtcc gctcgcgatc
     4981 tcgtcggcga cgcgccatcc cgcgctgccg gacctgccca cgatcgccga gaccctgccc
     5041 ggcgtggaga tgacgtcatg gttgggattc gtcggcccgg ccggcacgcc gcggccggtg
     5101 gtcgaccggc tcaacgccga gacccgcgcc gtcgtcgcgc tgcccgaggt gcagcgaaag
     5161 ctcgccgacc tgtcgggcgt gccgacccct tcgagccccg aggaaatgcg cgcgctggtc
     5221 gagcgcgaca tcgcacgctg gacgcgcgtg gtcgagctga agaagatccc gaagcagaat
     5281 tgaccggcga aggccggtca tcctgagcgc agcgaaggat ccgctttttc gcctacgccg
     5341 gcggcctgcg ccggagcagc ttcgcggcga ccgcggccag gccgagcacg gcgataccga
     5401 tgaccttggc gaacttcacc gcgaacgcgg cgatcagcgc gagcagtccc agcttcttcg
     5461 ccgcgacgcc cccgatcagc gcgagcagcc cgaactcggc gacgcggtcg gtggacgaat
     5521 cgaaatccgc gtagcgcttg cccgggttgt actcgagcgc ggcgagcatc tggcgcgcga
     5581 ccggcttctg cgcctcgatc gagttcatgc cggtgacgag gttcatgctg atgtagccgt
     5641 cgcggccgag cgcgtaggtg ttgtagttga cgccgcgctc ggcgccgtcg ggctcgccga
     5701 gctcgcgcga ttgcgccgac cacacgaggc ggtgcgtgcc ggcgtcgtac gccggcggct
     5761 cgacccagcc gatcacctcg atctccggaa tatggcgcga gcggcggtcc tggttcgacg
     5821 ccgctgtgcc cttcttcagg ctctgcagca gctcgtcgga gttccagtcc cgggcgtcgt
     5881 cgtccttgac gtagccggac ttcacctgcc gcatgacgat gaaccagttg aaccggtcgg
     5941 ggaagatggc gccgagcagg tcgtcgcccg tgcggttgcc catcgcctgc agcaggcggc
     6001 cggcttcggc cggcgggaag tagacgtggc ccgcgggcag cttcagcacc gcctggtcga
     6061 gcagcttcac ctccgacggc cccgcgacct gcgccttctg cgccgcctcg agcgccgcgc
     6121 ggatctcctc ctgcgggtcc tgcccgcgcg acgcaacagg cagcgccgcc gcgagcacgc
     6181 acagcagcgc gcaaagactc cgagtcatgg gtccctccct tgaaggacac gtccgggttc
     6241 ggggattatc ccgcccgcat ggacaccgtc ccgatcttcc cgggacggtt cgtgaccgat
     6301 tacgcggtgc tctgcatcga cgggtcgtac tcgccgtacg catcgtcggc gaagcagtac
     6361 agccagtggt cgcccggctc cgccgacgcg atcaccgggt gcccgctcgc gcgcgcgtga
     6421 cggctcgcgt ggcggttggg cgaggagtcg cagcacagcg tcgcgccgca cgtctggcac
     6481 gtgcgcagct gcacccagct cgatccggtc ttcacgcatt ccgcgcactg gcgcgtcttc
     6541 gggcgcacga cctcgacgat gtccttgatg tgctggcagg cttcggcagg catggtgttt
     6601 cctcctttca gggcgtgttc ggacgcggac tggcgatcgc gcccgcgatg tcgcgcgcga
     6661 cgcgcgcgag cgcgcggctg tacgcggccg cgatgccggc ggggtcgccg ggcgcgggct
     6721 cgtcggcgct ggagcgccct
//
LOCUS       DATDMW010000222         5236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1687488, whole genome shotgun sequence.
ACCESSION   DATDMW010000222 DATDMW010000000
VERSION     DATDMW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687488"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VI319_09650"
     CDS             <1..535
                     /locus_tag="VI319_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6864153.1"
                     /translation="MPEANAIEVMNRWAGKPLPVSATAFAGKQLAVRLSGAAAAVKAA
                     CAKIGGERLGDAEAAALWLAVRDHAAPFFESRPGGTPLWRIAVPSATPPLGVPGETMI
                     EWGGGQRWLVTHADARTVRDAARAAGGHATLFRYGDKSVGVFQPLEPAVAAIHRRLKQ
                     EFDPAGIFNPGRMYPNL"
     gene            542..1771
                     /gene="glcF"
                     /locus_tag="VI319_09655"
     CDS             542..1771
                     /gene="glcF"
                     /locus_tag="VI319_09655"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY6864154.1"
                     /translation="MQTALADFIRGTPEGDEADAILRKCVHCGFCTATCPTYLLLGDE
                     NDSPRGRIYLVKELLEGAEVSGKTQLHLDRCLTCRACETTCPSGVQYGRLADIGRKLV
                     EERVPRSPWQRAKRAALAAVLPRRALFSALYRVGRALRPLLPAALRRSIPETVAEPGA
                     WPAPRHARKMLALAGCVQPSLAPDTNAAAARLLDRIGISLVEAPGAGCCGALRFHMND
                     QAAGLNDMRALIDAWWPYVQSRAVEAIVMTASGCGVTVKEYGHLLRDDPRYAERASKI
                     SAMTRDLAEVLDAELDRLAPLFAGSKGKKIAFHPPCTLQHGQRVKGAAERVLARCGFE
                     LTPVPDAHLCCGAAGTYTILQPELSEQLKQNKLSALASGGPELALTANVGCQVHLDSA
                     GTLPVKHWVVALEERIS"
     gene            complement(1764..2438)
                     /locus_tag="VI319_09660"
     CDS             complement(1764..2438)
                     /locus_tag="VI319_09660"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY6864155.1"
                     /translation="MFYEPDKDDHGLPFNPYKSIVVPRPIGWISTLSRDGVANLAPYS
                     QFNNLGYDPPYVMFSANSLPGSGRRKDSVRNAIDTGEFVVNVATYELREAVNITSQFV
                     PPDTDEAALAGLEMIASRLVRAPRVAASPVHLECRFQCSLVLPANAFDQVHEVVVGRV
                     VGVHISDAVLTPEGKIDVLKIRPLARLGYFDYTTVESVFTMRPSGPDLAAREAGLEGR
                     PSRRAS"
     gene            complement(2438..3412)
                     /locus_tag="VI319_09665"
     CDS             complement(2438..3412)
                     /locus_tag="VI319_09665"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864156.1"
                     /translation="MRSTQLVACVLILGMAAGPTAASGAGAAEAWPQRTVRLIVPFGS
                     GTGPDVAARLYAERLGARWHQAVIVENRTGADGLVGATTFAAMRDDHALLFSPAQPIS
                     VFPYTLEKIAYDPVRDFVPISEAVDTFGSIAVSSSLAVRSLAELVALARAQSGERNWT
                     SGGGAFPTLLAGFARTAGLDMVQVSYREQNLALQDLAEGRVQILATTLTALLPYAKAG
                     KIRLLAVTNKSRAPMAPDVPTATEAGFPGLAFEGLIGFFGGRDMPPALRDRISEDVRA
                     VAADPVLVERLARAGQVAHAGTPAQFGGAIEEQRARIAALARLVREAR"
     gene            3512..4519
                     /locus_tag="VI319_09670"
     CDS             3512..4519
                     /locus_tag="VI319_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6864157.1"
                     /translation="MVKPLDWDSRIGRRIRLRDLHILFSVVQHGSMAKAGGHLGMSQS
                     AVSQAIAALENALEVRLLDRTARGVEPTMYGEALMRRGQAAFDELRSGVRDIESLTDP
                     RRGEIRVACPESIAAGLLPIVIERFSRSYPEARLHVFQTTTHLMGFAALHERKADLVI
                     TLLPKPFEDDLTEYLQAEVLFQDSICLAAGTRSPWARRARVQLADLVDAELVLPDPDA
                     PGAAAVIEAFRAAGLPEPRTTVTTFSVHVRSILSMRGRFVAVLPTSVLRLNPGLYPLK
                     ELPLDLPMPQWPALIVTLKHRTLSASVERFIECARDVAGEFHAAPPARGSGMIRRGRK
                     R"
     gene            4527..>5236
                     /locus_tag="VI319_09675"
     CDS             4527..>5236
                     /locus_tag="VI319_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY6864158.1"
                     /translation="MARQIDNLPERPASRDVRQLARVLRFVAPYKARIAVAGVALVVA
                     AGCVLVLGQGLKRVIDSGFASADPASLDTALFALIAIVAVLAAATYTRFYSVSWIGER
                     VSADIRRAVFGHLLEMPPGFFEATRTGEVISRLTSDTALLELVIGSSASFALRNTLIM
                     LGALAMLVITSPKLTALVVLGVPLVVVPILLYGRRVRRLSRATQDRIADVSAHVDESL
                     HEIRTVQAYGHEDADREQ"
BASE COUNT          769 a         1825 c         1878 g          764 t
ORIGIN      
        1 gatgcccgag gcgaacgcga tcgaggtcat gaaccgctgg gcgggcaagc cgctgccggt
       61 gagcgcgacc gccttcgcgg gcaagcagct cgcggtgcgg ctctcgggcg cggcggccgc
      121 ggtgaaggcc gcgtgcgcga agatcggcgg cgagcggctc ggcgacgcgg aagcggcggc
      181 gctctggctc gcggtgcgcg accacgcggc gccgttcttc gagtcgcgcc ccggcggcac
      241 gccgctgtgg cgcatcgccg tgccgtcggc gacgccgccg ctcggcgtcc ccggcgagac
      301 gatgatcgag tggggcgggg gccagcgctg gctcgtgacg cacgccgacg cgcgcacggt
      361 gcgcgacgcg gcgcgcgcgg ccggcgggca cgcgacgctc ttccgctacg gcgacaagtc
      421 ggtcggcgtg ttccagccgc tcgagcccgc cgtcgcggcg atccaccggc ggctcaagca
      481 ggaattcgat ccggccggca tcttcaatcc cgggcggatg tacccaaatc tctaggttca
      541 gatgcaaacc gcattagccg atttcatccg gggaactccc gagggcgacg aagccgacgc
      601 catccttcgc aagtgcgtgc attgcggctt ctgcaccgcg acctgcccga cctacctgct
      661 gctcggagac gagaacgaca gcccgcgcgg ccgcatctac ctggtgaagg agttgctcga
      721 gggcgccgag gtcagcggga agacgcagct ccatctcgac cgctgcctca cgtgccgcgc
      781 ctgcgagacg acctgtccgt cgggcgtgca atacggccgc ctcgccgaca tcgggcgcaa
      841 gctcgtcgag gagcgcgtgc cgcgctcgcc ctggcagcgc gcgaagcgcg cggcgctcgc
      901 cgcggtgctg ccgcgccgcg cgctgttctc ggcgctgtat cgcgtcggac gcgcgctgcg
      961 gccgctgctt cccgccgcgc tccggcgcag catcccggag accgtcgccg agcccggcgc
     1021 ctggcccgcg ccgcgccacg cgcgcaagat gctcgcgctc gcgggttgcg tgcagccgtc
     1081 gctcgcgccc gacaccaacg ccgcggcggc gcggctgctc gaccggatcg gcatctcgct
     1141 cgtcgaagcg ccgggcgccg gctgctgcgg cgcgctgcgc ttccacatga acgaccaggc
     1201 cgctggctta aacgacatgc gcgcgctgat cgacgcatgg tggccctatg tccagagccg
     1261 cgccgtcgag gcgatcgtga tgaccgcgag cggctgcggg gtgacggtga aggagtacgg
     1321 ccacttgctg cgcgacgacc cgcgctacgc cgagcgcgcg tcgaagatca gcgcgatgac
     1381 gcgcgacctc gccgaggtgc tcgacgcgga gctcgaccgg ctcgcgccgc tcttcgcggg
     1441 ctcgaaagga aagaagatcg cgttccaccc gccctgcacg ctgcagcacg gccagcgcgt
     1501 gaagggcgcg gccgagcgcg tgctcgcgcg ctgcggcttc gagctcacgc ccgtgcccga
     1561 cgcgcacctg tgctgcggcg cggccggcac ctacacgatc ctccagcccg agctctccga
     1621 gcagctgaag cagaacaagc tctcggcgct cgcctcgggc gggcccgagc tcgcgctcac
     1681 cgcgaacgtc ggctgccagg tgcacctcga ctccgcgggc acgctgccgg tgaagcactg
     1741 ggtcgtggcg ctggaagagc gcatcagcta gcgcgacggc tgggccggcc ctccagcccg
     1801 gcttcgcgcg cggcgaggtc gggccccgag ggccgcatcg tgaacaccga ctcgaccgtc
     1861 gtgtaatcga aatatccgag gcgcgccagg ggacggatct tcagtacgtc gatcttgcct
     1921 tcgggcgtca gcacggcgtc gctgatgtgc acgccgacga cgcgcccgac cacgacttcg
     1981 tgcacctggt cgaacgcgtt cgccggcagc acgagcgagc actggaagcg gcactccagg
     2041 tgcaccggcg acgccgcgac gcgcggcgcg cgcacgagac gggaggcgat catctcgagg
     2101 ccggcgagcg cggcctcgtc ggtgtccggc gggacgaact gcgacgtgat gttgaccgcc
     2161 tcgcgcagct cgtaggtcgc gacgttcacg acgaactcgc cggtatcgat cgcgttcctc
     2221 acgctgtcct tgcggcgccc gctgccggga agcgagtttg ccgagaacat gacgtaaggc
     2281 gggtcgtatc cgaggttgtt gaactggctg tagggcgcga ggttcgcgac gccgtcgcgg
     2341 ctcagcgtgg agatccagcc gatcggtcgc ggaacgacga tcgacttgta cgggttgaac
     2401 ggcagcccgt gatcgtcctt gtcgggctcg tagaacatca gcgcgcttcc ctgaccaggc
     2461 gggcgagggc ggcgatcctg gcacgctgct cctcgatggc gccgccgaac tgggccgggg
     2521 tgcccgcgtg ggccacctgt cccgcccggg ccagccgctc gaccagcacg ggatccgccg
     2581 ccaccgcgcg gacgtcctcc gaaatccggt ctcgcagcgc cggcggcatg tcccgcccgc
     2641 cgaagaatcc gatcagtcct tcgaacgcga gcccgggaaa tccggcttcg gtcgccgtcg
     2701 ggacatcggg cgccatcggg gcgcgcgact tgttggtgac cgcgagaaga cggatcttgc
     2761 cggccttcgc ataggggagc agggcggtga gcgtcgtggc gaggatctgg acccgtcctt
     2821 cggcgaggtc ctggagcgcg aggttctgct cgcggtagga aacctgcacc atgtcgagac
     2881 ccgcggtcct cgcgaagccg gcgagcagcg tggggaaggc gccgcccccg ctggtccaat
     2941 tgcgctcgcc cgattgcgcg cgggccagcg cgacgagctc ggcgagcgac cggaccgcga
     3001 gcgacgacga aacggctatc gagccgaagg tatcgacggc ctcggaaatg ggaacgaaat
     3061 cccggacagg gtcgtaggcg atcttctcca gggtgtacgg gaagacgctg atcggctgcg
     3121 caggcgagaa gagcagcgcg tgatcgtcgc gcatcgcggc gaaggtcgtc gcgccgacca
     3181 gaccgtcggc gccggtccga ttttcgacga tcaccgcctg gtgccatcgt gcacccaggc
     3241 gctccgcgta aaggcgcgcg gcgacgtcgg gaccggtccc gctcccgaag ggaacgatca
     3301 ggcggaccgt tcgctgcggc cacgcctccg cggcccccgc cccggaggcc gccgtcggac
     3361 cggcggccat tcccaggatc aggacgcagg cgaccagctg ggtgcttctc atcggaggcc
     3421 tccttggttc gggttggacg gttgaagcat agggtccgcg gtgctaaaag gagaatggcg
     3481 attttgagac atgggcatta gcaaaagcta aatggtgaag ccgctcgact gggacagccg
     3541 gatcggccgg cgcatccggc tgcgcgatct ccacatcctg ttctcggtcg tgcagcacgg
     3601 cagcatggcc aaggcgggcg gccatctcgg gatgtcgcag tccgccgtgt cccaggcgat
     3661 cgcagccctg gagaacgcgc tggaggtccg cctgctcgac cgcaccgcgc gcggcgtcga
     3721 gccgaccatg tacggagaag cgctgatgcg gcgcggccag gccgccttcg acgagctcag
     3781 gtccggcgtg agagacatcg agtccctgac ggacccgcgg cgcggcgaga tccgggtcgc
     3841 ctgtcccgaa tcgatcgcgg cggggctcct gccgatcgtg atcgagcgct tttcgcgcag
     3901 ctatcccgag gccaggctgc acgtcttcca gaccacgact cacctgatgg gattcgcggc
     3961 gctgcacgaa aggaaggcgg atctcgtcat cacgctgctg ccgaagccct tcgaggacga
     4021 cctgacggaa tatctgcagg ccgaggtcct gttccaggac agcatctgcc tggccgcggg
     4081 gacgcggagc ccgtgggcac gaagggcgag ggtgcagctc gccgacctgg tcgatgccga
     4141 attggtattg cccgacccgg acgcgccggg ggcggccgcc gtcatcgagg cctttcgcgc
     4201 cgccgggctg cccgagccgc ggacgacggt cacgacgttc tcggtgcacg tgcgcagcat
     4261 cctcagcatg cgggggcgct tcgtcgcggt gctgccgaca tcggttctcc ggctcaatcc
     4321 gggcctgtac ccgctgaagg agctgcccct cgacctgccg atgccgcaat ggcccgcgct
     4381 gatcgtcacg ctgaagcaca ggacgctgag cgcatcggtc gagcgcttca tcgaatgcgc
     4441 gcgcgacgtc gccggggaat tccatgccgc gccgccggcg cgcggatcgg gcatgatacg
     4501 gcgaggtcgg aagcgctgac ggtttcgtgg cacgacaaat cgacaatctc cccgagcgtc
     4561 cggcgagccg cgacgtccgg caactcgcgc gcgtgctgcg cttcgtcgcg ccgtacaagg
     4621 cgcggatcgc ggtcgcgggc gtcgcgctcg tggtcgccgc gggctgcgtg ctggtcctcg
     4681 gccaggggct gaagcgcgtc atcgactcgg gcttcgcgag cgccgatccc gcgtcgctcg
     4741 acacggcgct gttcgcgctg atcgcgatcg tcgcggtgct cgccgcggcg acgtacacgc
     4801 gcttctactc ggtctcctgg atcggcgagc gcgtctcggc cgacatccgg cgcgcggtgt
     4861 tcgggcacct gctcgagatg ccgccggggt tcttcgaggc gacgcgcacc ggcgaggtga
     4921 tctcgcggct cacgagcgac acggcgctgc tcgagctggt gatcggctcg tcggcctcgt
     4981 tcgcgctgcg gaacacgctg atcatgctcg gggcgctggc gatgctggtg atcacgagcc
     5041 cgaagctgac cgcgctcgtc gtgctcggcg tgccgctcgt cgtggtgccg atcctcctct
     5101 acggccggcg cgtgcggcgc ctgtcgcgcg cgacgcagga ccgcatcgcc gacgtcagcg
     5161 cgcacgtcga cgagtcgctg cacgagatcc gcacggtgca ggcctacggc cacgaggacg
     5221 ccgaccgcga gcagtt
//
LOCUS       DATDMW010000223         4563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1438308, whole genome shotgun sequence.
ACCESSION   DATDMW010000223 DATDMW010000000
VERSION     DATDMW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438308"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VI319_09680"
     CDS             <1..623
                     /locus_tag="VI319_09680"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY6864159.1"
                     /translation="LTNGPRGGLRAKGTLYTIDGTYVAFGQRLTIDRGRIIFDGPLDN
                     PALDVVALRKNLPVEAGVQISGTAKLPQVQLTSNPPVPDGEKLSWLVLGRAPDDANKA
                     DLALLQAAAGALLPRGSSVPLTRRIARTVGLDEITLRGNSELAERVVALGKRVSDRLY
                     VSYEQAVGATAANLVKLDYTLSRRWSVRAETGTITGLGLFYRYSWD"
     gene            complement(663..1106)
                     /locus_tag="VI319_09685"
     CDS             complement(663..1106)
                     /locus_tag="VI319_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004523329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6864160.1"
                     /translation="MKVSEAMTRDVMLILADQSIQEAAALMEDCDIGSLPVADSDRLI
                     GMITDRDIAVRAVAKGQPPDTPVSQVMSQEVLYCFEDDDVKNVAKNMSDIQVRRLPVV
                     TRDKKLVGILSLGDLARKSESRTAGEAMKGISKPSDQHSQTAGAH"
     gene            1259..1345
                     /locus_tag="VI319_09690"
     tRNA            1259..1345
                     /locus_tag="VI319_09690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1293..1295,aa:Leu,seq:taa)
     gene            1367..1759
                     /locus_tag="VI319_09695"
     CDS             1367..1759
                     /locus_tag="VI319_09695"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEY6864161.1"
                     /translation="MTIRIVRLGTPRARAEGLRLGTVRKLPRGVRKTDYARLDWFDLW
                     LPELAPSDKLFAFARAAPLEGKRWTGFARRYRREMAAPGAGRILEMLAHLSRQADFSV
                     GCYCEDESRCHRSVLRELLRERGAKIAA"
     gene            1784..2758
                     /locus_tag="VI319_09700"
     CDS             1784..2758
                     /locus_tag="VI319_09700"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY6864162.1"
                     /translation="MAETESWAFPGNLQPDPADYDFPLAAALDALVLVRTEIPEDAFT
                     APILGTERAGNGVVIRDDGLVLTIGYLITEASAVWLTTSRGVAVAGTPLAYDQATGFG
                     LVQPLGRLGVPARARGSAASCSVGDEVIVAGHGGRAHALKANVVARREFAGYWEYVLD
                     EAIFTAPAHPQWGGSALSGDQGKLLGIGSLLVQEQVDDKNVQGNMIVPIDLIEPILGD
                     LLKFGARSGAPRAWLGLYASEVRGMLAVAGVAPRGPADRAQIRTGDLVLEVGGRRVTA
                     LADLFRAVWQLGPAGTDVPLTISRDGKVSHVSVRSGDRHDFLKKPHLH"
     gene            2792..3232
                     /locus_tag="VI319_09705"
     CDS             2792..3232
                     /locus_tag="VI319_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY6864163.1"
                     /translation="MIVFELICPDRHRFEGWFASSEDFHGQRAAGLLSCPVCSSPGVE
                     KLLTAKIAKGEPPREGRPNVSNVKPAPGGAKDRPTLKDVVDFILANTEDVGKAFPEQA
                     RRMHYKEAPHRNIRGVATLEETHELIEEGIDVVPLPVPPRGELN"
     gene            complement(3247..>4563)
                     /locus_tag="VI319_09710"
     CDS             complement(3247..>4563)
                     /locus_tag="VI319_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864164.1"
                     /translation="PNTVLAREHPAHFRLDGRYFAACDESGAPLKPQYSISKPERPDV
                     LLSRLPDGRFVSYFGDLHPSYFGNVVKALGSARQGYPVVSRVLAKKQPSSGEQPSKFF
                     ADLARELLATVHEVKRLTPTIVEVVVRAPLAARKFEPGQFYRLQNFETLSPLVSGSAG
                     ATRMQMEGLALTGAWVDKSRGLISTIVLEMGGSSDLCAMLKPGEPVVLMGPTGTPTHI
                     AANETVILAGGGLGNAVLFSIGQAFRARGSKVLYFAGYKKVVDRYKVAEIENAADVVV
                     WCCDEEPGFRPTRPQDRTFVGNIVQAMDAYAEGRLGRVTLRMDDADRLIAIGSDRMMA
                     AVGRARHDVLKPHLKPHHFAIGSINSPMQCMMKEICAQCLQPHHDPRTGKTSYVFSCF
                     NQDQELDCVDFGALSERLRQNSLQEKLAAQWIRHCLPELAKQRERV"
BASE COUNT          684 a         1604 c         1578 g          697 t
ORIGIN      
        1 agctcacgaa cggcccgcgc ggcggcctcc gagccaaggg gacgctctac acgatcgacg
       61 ggacctacgt tgccttcggc cagcgcctca ccatcgatcg cgggcgcatc atcttcgacg
      121 gtccgctcga caatccggcc ctcgacgtgg tggcgctgcg caagaacctt ccggtggaag
      181 ccggcgtgca gatcagcggc acggcgaagc tcccgcaggt gcagctcacg tcgaatccgc
      241 ccgtgccgga cggcgagaag ctgtcgtggc tggtgctcgg gcgcgcgccc gacgacgcca
      301 acaaggccga cctcgcgctg ctgcaggccg cggcgggcgc gctactgccg cgcggcagct
      361 cggtgccgct cacgcggcgc atcgcgcgca ccgtgggcct ggacgagatc acgctgcgcg
      421 ggaacagcga gctcgccgag cgcgtggtgg cgctcggcaa gcgcgtctcg gaccggctct
      481 acgtcagcta cgaacaggcc gtcggcgcga ccgccgcgaa cctcgtgaag ctcgactaca
      541 cgctgtcgcg gcgctggtcg gtgcgcgccg agaccgggac gatcaccgga ctcggcctgt
      601 tctaccgata ctcctgggat tgaccgcgcc aaggcgcggt catccccgcg aaagcgggga
      661 tctcagtgcg cgccggcggt ctgcgagtgc tgatcgctcg gcttggagat gcctttcatc
      721 gcctcgccgg ccgtgcggct ctcggacttg cgcgcgaggt cgccgagcga caggatgccg
      781 accagcttct tgtcgcgcgt cacgacgggc aggcggcgca cctggatgtc gctcatgttc
      841 ttcgcgacgt tcttcacgtc gtcgtcctcg aagcagtaga gcacttcctg gctcatcacc
      901 tggctcaccg gcgtgtcggg cggctggcct ttcgcgaccg cgcgcaccgc gatgtcgcga
      961 tcggtgatca tgccgatgag gcggtcgctg tcggcgaccg gcagggaccc gatatcgcaa
     1021 tcctccatca gggccgcggc ctcctggatc gattgatcgg caaggatgag catcacgtcg
     1081 cgggtcatcg cttcgctgac tttcatgggg actcctttcg tcgttgccgc cggagcaatc
     1141 atgcggtcgc tccggacgtg tatgtttccg ctccgcgtat cgtcgcgcgc ggccgcaccc
     1201 gccatagacc cgcttcgcgg cgcgatgttt cagcgacttg tgggacaatg cgtgcgccgc
     1261 ctgggtggcg aaactggtag acgcaaggga cttaaaatcc ctcgggggcg accccatgcc
     1321 ggttcgagtc cggccccggg caccatcgcg agcgattcga ccccgcatga cgatacgtat
     1381 cgtgcgcctc ggcacgccgc gcgcgcgggc cgaaggcctg cgcctcggaa cggtgcgcaa
     1441 gctcccacgc ggcgtgcgca agaccgatta cgcgcggctg gactggttcg atctctggct
     1501 gcccgagctc gcgccgagcg acaagctgtt cgcgttcgcg cgcgcggcgc ctctcgaagg
     1561 aaagcgctgg acgggcttcg cgcggcgcta tcgccgcgag atggccgcgc ccggcgcggg
     1621 ccggatcctc gagatgctcg cgcacctctc gcgccaggcc gatttttccg tcggctgcta
     1681 ctgcgaggac gagagccgct gccaccgctc ggtgctgcgc gagcttttgc gcgagcgcgg
     1741 cgctaagata gcggcgtgaa atcgcggcga aagaacaact agcgtggccg aaaccgagag
     1801 ctgggccttc ccgggcaacc tgcagcccga tcccgccgac tacgatttcc cgctcgccgc
     1861 ggcgctcgac gcgctggtgc tcgtgcgcac cgagatcccg gaggacgcgt tcaccgcgcc
     1921 catcctcggc accgagcgcg cgggcaacgg cgtggtgatc cgcgacgacg gcctggtgct
     1981 cacgatcggc tacctgatca ccgaggcgag cgcggtgtgg ctcaccacga gccgcggcgt
     2041 cgccgtcgcc ggcacgccgc tcgcgtacga ccaggcgacc ggcttcggcc tggtgcagcc
     2101 gctcgggcgg ctcggcgttc ccgcgcgcgc gcgcgggtcg gcggcctcgt gctcggtggg
     2161 cgacgaggtc atcgtcgccg gccacggcgg acgcgcgcac gcgctgaagg cgaacgtggt
     2221 cgcgaggcgc gagttcgcgg gctactggga atacgtgctc gacgaggcga tcttcaccgc
     2281 gcccgcgcat ccgcagtggg gcggctcggc gctgagcggc gaccagggca agctgctcgg
     2341 catcggatcg ctgctggtgc aggagcaggt cgacgacaag aacgtgcagg gcaacatgat
     2401 cgtcccgatc gacctcatcg agcccattct cggcgacctg ctcaaattcg gagcgcgctc
     2461 gggcgcgccg cgcgcgtggc tcgggctgta cgcgagcgaa gtgcgcggca tgctggccgt
     2521 cgccggcgtc gcgccgcgcg gacccgcgga ccgcgcgcag atcaggaccg gcgacctcgt
     2581 gctcgaagtc ggcggacggc gcgtcaccgc gctcgccgac ctgttccgcg cggtctggca
     2641 gctcggcccc gcgggcaccg atgtgccgct caccatcagc cgcgacggca aggtatcgca
     2701 cgtcagcgtg cgctcgggcg accgccacga cttcctgaag aagccgcatt tgcactgaaa
     2761 acggcgtgcg attgccggta caatcgccgg catgatcgta ttcgagctga tctgccccga
     2821 ccgccatcgc ttcgagggct ggttcgcgtc ttcggaggat ttccacggac agagagccgc
     2881 cggcctgctt tcgtgcccgg tgtgcagcag tcccggcgtc gagaagctgc tcaccgccaa
     2941 gatcgcgaag ggcgagccgc cgcgcgaagg ccggccgaac gtctccaacg tgaagcccgc
     3001 gccgggcggc gccaaggacc ggcccacgct caaggacgtc gtcgatttca tcctcgccaa
     3061 caccgaggac gtcggcaagg cgttcccgga gcaggcgcgg cgcatgcatt acaaggaagc
     3121 gccgcaccgg aacatccgcg gcgtcgccac gctcgaggag acgcacgagc tgatcgagga
     3181 ggggatcgac gtggtcccgc tgccggtccc gccgcgcggc gagctgaact gaggagctga
     3241 gccgggctag acgcgctcgc gctgcttcgc gagctccggc aggcaatgcc ggatccactg
     3301 cgcggcgagc ttctcctgca gcgagttctg cctgagccgc tcggacagcg cgccgaagtc
     3361 gacgcagtcg agctcctggt cctggttgaa gcaggagaac acgtagctcg tcttccccgt
     3421 gcgcgggtcg tggtgcggct gcaggcactg cgcgcagatc tccttcatca tgcactgcat
     3481 cggcgagttg atcgagccga tcgcgaagtg gtgcggcttg aggtgcggct tcagcacgtc
     3541 gtggcgcgcg cggccgaccg ccgccatcat gcggtcggag ccgatcgcga tcagccggtc
     3601 cgcgtcgtcc atccgaagcg tgacgcgccc gaggcgcccc tcggcgtacg catccatcgc
     3661 ctgcacgatg ttgccgacga aggtcctgtc ctgcggtcgc gtcggccgga agcccggctc
     3721 ctcgtcgcag caccacacga cgacgtcggc cgcgttctcg atctcggcga ccttgtagcg
     3781 gtcgaccacc ttcttgtagc cggcgaagta gagcaccttc gagccgcgcg cgcggaacgc
     3841 ctggccgatc gagaacagca ccgcgttgcc caggccgccg cccgcgagga tcaccgtctc
     3901 gttcgccgcg atgtgcgtcg gcgtcccggt cgggcccatg agcaccaccg gttcgcccgg
     3961 cttcagcatg gcgcagagat ccgaagagcc gcccatctcg agcacgatcg tcgagatcag
     4021 cccgcgggac ttgtcgaccc atgcgccggt caacgccagc ccctccatct gcatcctcgt
     4081 cgcgcccgcc gagccgctca ccaggggcga gagcgtctcg aagttctgca gccggtagaa
     4141 ctgtcccggc tcgaacttgc gcgcggcgag cggggcgcgc accaccacct cgacgatggt
     4201 cggcgtcagg cgcttcacct cgtgcaccgt cgcgagcagc tcgcgcgcga ggtccgcgaa
     4261 gaacttcgac ggctgctcgc ccgagctcgg ctgctttttc gccagcacgc gcgagaccac
     4321 cgggtacccc tgccgcgcgg agccgagcgc cttcaccacg ttgccgaagt aggagggatg
     4381 caggtcgccg aagtagctga cgaagcggcc gtcgggcagg cgcgagagca ggacgtcggg
     4441 ccgctcgggc ttggagatcg agtactgcgg cttgagcggc gccccgctct cgtcgcacgc
     4501 cgcgaagtag cgcccgtcga ggcggaaatg cgccgggtgc tcgcgcgcga gcacggtgtt
     4561 ggg
//
LOCUS       DATDMW010000224         3458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_69806, whole genome shotgun sequence.
ACCESSION   DATDMW010000224 DATDMW010000000
VERSION     DATDMW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69806"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VI319_09715"
     CDS             complement(<1..469)
                     /locus_tag="VI319_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY6864165.1"
                     /translation="MTAYPVKVQDLLLNSPQFAELGKKDLARLAAATSRVRAPAGTVL
                     FRRGEPCAGVYVIVYGQVKLAVHGAEGAEKILEILGPGQTFGEPFMFLDEPHLSFAET
                     LADSLLLLVGKAAVLQTIAGNAHFAKTMLASMAQRLQRVFTDIEAYTLKSGSQR"
     gene            complement(498..1082)
                     /locus_tag="VI319_09720"
     CDS             complement(498..1082)
                     /locus_tag="VI319_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY6864166.1"
                     /translation="MSKALSTIRDEHRSIGAILHGMEYLVGEIRARKAKVDPRVFATM
                     LYYLDTFSERYHHPKEDRYLFAPLKRHGPEAHGVVAQLEHEHAQGGQALRRLEQYLVR
                     YEEGGDREFPAFARAVDEFVQGYWEHMRKEEEDLFPLAEKLLTADDWHVVDAAFAENR
                     DPLAAERDTRDYQKLFTRIVNIAPPPIGVGPQSR"
     gene            1215..1661
                     /locus_tag="VI319_09725"
     CDS             1215..1661
                     /locus_tag="VI319_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6864167.1"
                     /translation="MYKHILVATDGSKLSGKAVRAAARLAKTCKARLTAVYAVQPYPD
                     PVYSEGAAFGPRMSRTRYTELAEREGRKALAAVEIEAQTAGVPYGSVLATDAQAWKAI
                     IRAARAKRCDVVVMASHGRRGLSAMLLGSETSKVLTHSRVPVLVCR"
     gene            1667..2059
                     /locus_tag="VI319_09730"
     CDS             1667..2059
                     /locus_tag="VI319_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY6864168.1"
                     /translation="MRYLTLEQRESLVERLQRRAEALYDEVRAGLHPAERPGDVGFAN
                     RRDETDDEAVADLESSLDVSAVARDVLELHEVERALRRIHTGEYGTCHECGADIPYAR
                     LAAQPTALRCTACQDRHERERGTTVRTL"
     gene            complement(2056..>3458)
                     /locus_tag="VI319_09735"
     CDS             complement(2056..>3458)
                     /locus_tag="VI319_09735"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEY6864169.1"
                     /translation="ERASPSTAARRAEWTLERDYPFGASFLAMCHAWRSPAGEARVVV
                     KGAPETVLPLCAMEPEERNAALEEAASAAARGVRLLAVGEAPWGAAAWPDDPSRYGFR
                     WLGFVALADPLRASVPEAVALCRRAGIRVVMITGDHAATAQAIARQAGIPVDTGVLTG
                     AELARMDDAALAAAVRRTCVFARILPEQKLRLVAAYKADGEVVAMTGDGVNDAPALKA
                     AHIGVAMGRRGTDVAREAAALVLLEDDFGSIVHTVRLGRRIYANIRNAMSYLVAVHVP
                     IAGMSFLPVVFGWPVFLFPVHVVFLEFVIDPACSIVFEAEETDADAMERPPRAPAERL
                     FGARMLAGSLALGAAVLVVTCAAYAWMAHSGRAEAEVRGFGFAAIVFGNLALILASRS
                     RDRTIAETLARPNRALWWVVLGTLAALAASLYVPEVAALFRFGPLGAAELGAAFAAGT
                     AAVIVGEAVKRSRRPA"
BASE COUNT          523 a         1270 c         1229 g          436 t
ORIGIN      
        1 cgcgctgcga gccgctcttc agggtgtagg cctcgatgtc ggtgaacacg cgctgcaggc
       61 gctgcgccat gctcgcgagc atcgtcttgg cgaagtgcgc gttgcccgcg atggtctgca
      121 gcaccgccgc cttgccgacg aggagcagca gcgagtcggc gagcgtctcg gcgaagctga
      181 ggtgcggctc gtcgaggaac atgaagggct cgccgaaggt ctgaccgggc ccgaggatct
      241 cgaggatctt ctcggcgccc tcggcgccgt gcacggcgag cttcacctgc ccgtagacga
      301 tgacgtagac gccggcgcac ggctcgccgc ggcggaaaag gaccgtgccc gctggcgcgc
      361 gcacgcggct cgtcgccgcg gcgaggcgcg cgaggtcctt cttgccgagc tcggcgaact
      421 gcggcgagtt cagcagcaga tcctgcacct tcaccggata cgcggtcatg agcaagggga
      481 cctccgtcgg ggacggatca gcgtgactgg gggccgacgc cgatcggcgg cggcgcgatg
      541 ttgacgatgc gcgtgaacag cttctggtag tcgcgcgtgt cgcgctcggc ggcgagcggg
      601 tcgcgattct cggcgaaggc ggcgtcgacc acgtgccagt cgtcggccgt gagcagcttc
      661 tcggcgagag gaaacagatc ctcctcctcc ttgcgcatgt gctcccagta gccctggacg
      721 aactcgtcga cggcgcgggc gaacgcgggg aactcgcggt cgccgccttc ctcgtagcgg
      781 accaggtact gctcgagacg ccgcagcgcc tgcccgccct gcgcgtgctc gtgctcgagc
      841 tgcgcgacca cgccatgcgc ctcgggaccg tgccgcttga gcggcgcgaa caggtagcgg
      901 tcctccttcg ggtggtgata gcgctccgag aacgtgtcca ggtagtacag catcgtcgcg
      961 aacacgcgcg ggtcgacctt ggccttgcgc gcgcggatct ccccgaccag gtattccatg
     1021 ccgtgcagga tcgcgccgat cgagcggtgc tcgtcgcgga tcgtcgaaag cgccttcgac
     1081 atcgtcatct cctccgggcg tctggccatg gtggccaaat ctagccttcg cgcggaaagc
     1141 ggatttgaca cagatcaaag gggcgtgacg gagcggcgct atcgtgagct ccaaggcaag
     1201 aaaggagcaa gaacatgtac aagcacatcc tggtggcgac cgacggcagc aagctctcgg
     1261 gcaaggcggt gagggccgcc gcgcgcctgg cgaaaacctg caaagcccgg ctcaccgcgg
     1321 tctacgcggt gcagccctac cccgacccgg tctacagcga aggcgccgcc ttcgggccac
     1381 ggatgtcgcg cacccgctac accgagctgg ccgagcgcga gggccgcaag gcgctcgccg
     1441 ccgtcgagat cgaggcgcag actgccggcg ttccgtacgg ctcggtgctc gccaccgacg
     1501 cgcaggcctg gaaggccatc atccgcgccg cgcgcgcgaa gcgctgcgac gtcgtcgtca
     1561 tggcctcgca cgggcgccgc ggcctctcgg cgatgctgct cggcagcgaa accagcaagg
     1621 tgctcacgca ctcgcgcgtg cccgtcctcg tgtgccggtg acggccgtgc gctacctcac
     1681 gctcgagcag cgcgagtccc tcgtcgagcg gctgcagcgg cgcgccgagg ccttgtacga
     1741 cgaagtgcgc gccggcctgc atccggccga gcggcccggc gacgtcggct tcgccaaccg
     1801 gagggacgag accgacgacg aggcggtcgc cgacctcgag tcctccctcg acgtctcggc
     1861 cgtcgcgcgc gacgtgctcg agctgcacga ggtcgagcgc gccctgcggc gcatccacac
     1921 gggcgagtac ggcacctgcc acgaatgcgg cgccgacatc ccgtacgcgc gtctggccgc
     1981 gcagccgacc gcgctgcgct gcaccgcctg ccaggatcgc cacgagcgcg agcgcgggac
     2041 gaccgtgcgc acgctctagg cgggtcgccg cgaccgtttg acggcctcgc cgacgatcac
     2101 cgcggccgtc cccgccgcga acgccgcccc cagctcggcg gccccgagcg ggccgaagcg
     2161 gaacagggcc gcgacctccg gcacgtagag cgacgcggcg agcgcggcga gcgttccgag
     2221 caccacccac cacagcgccc ggttcgggcg cgcgagcgtc tcggcgatcg tgcggtcgcg
     2281 cgagcggctc gcgaggatca acgccagatt cccgaacacg atcgcggcga agccgaagcc
     2341 gcgcacctcg gcctccgcgc gcccggagtg cgccatccag gcgtacgcgg cgcaggtgac
     2401 gaccagcacc gcggcgccca gcgccaggct tccggccagc atgcgcgcgc cgaagagccg
     2461 ctcggcgggc gcgcgcggcg gccgctccat cgcgtcggcg tcggtctcct cggcctcgaa
     2521 cacgatcgag cacgcgggat cgatgacgaa ctcgaggaac accacgtgca ccgggaacag
     2581 gaacaccggc cagccgaaca cgaccgggag gaacgacatg cccgcgatcg gcacgtgaac
     2641 ggcgacgagg tacgacatgg cgttgcggat gttggcgtag atgcgacggc ccaggcgcac
     2701 ggtgtgcacg atcgagccga aatcgtcctc gagcagcacc agcgccgccg cctcgcgcgc
     2761 gacgtcggtg ccgcgccggc ccatcgccac gccgatgtgc gcggccttga gcgcgggcgc
     2821 gtcgttcacg ccgtcgccgg tcatggcgac cacctcgccg tcggccttgt acgcggcgac
     2881 gagccgcagc ttctgctcgg gcaggatgcg cgcgaacacg cacgttcggc ggaccgccgc
     2941 ggcgagcgcg gcgtcgtcca tgcgcgcgag ctcggcgccg gtcagcacgc ccgtgtcgac
     3001 cgggatcccg gcctggcgcg cgatcgcctg cgccgtggcg gcgtgatcgc ccgtgatcat
     3061 caccacgcgg atgcccgcgc ggcggcacag cgccaccgcc tcgggcaccg aagcgcgcag
     3121 cggatcggcg agcgcgacga agcccagcca gcggaagccg taccgcgacg ggtcgtcggg
     3181 ccaggcggcc gcgccccacg gcgcctcgcc cacggcgagc agccgcaccc ccctggccgc
     3241 cgccgatgcc gcctcctcga gcgccgcatt ccgctcctcg ggctccatcg cgcaaagcgg
     3301 caggacggtc tccggcgcgc ccttcaccac gacgcgcgcc tcgcccgcgg gcgagcgcca
     3361 ggcgtggcac atggcgagga aggaggcgcc gaacggatag tcgcgctcga gcgtccactc
     3421 cgcgcgccgc gccgcggtcg acgggctcgc gcgctccg
//
LOCUS       DATDMW010000225         4231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_566297, whole genome shotgun sequence.
ACCESSION   DATDMW010000225 DATDMW010000000
VERSION     DATDMW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566297"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VI319_09740"
     CDS             complement(<1..359)
                     /locus_tag="VI319_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6864170.1"
                     /translation="MFRWLFGNRRASEPAAAVAGAPSIEEALGHHRAGRLAEADALYR
                     RILESDPANIDAAHFLGVLAYQRGDLAQAAERISAALARNPANAPAQNNLGNVLDAQG
                     RHDDALRCYLEALALEP"
     gene            462..674
                     /locus_tag="VI319_09745"
     CDS             462..674
                     /locus_tag="VI319_09745"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEY6864171.1"
                     /translation="MRLLLIALAVTLVYVIVRGMTRASAPRRPTRPVVERMVPCAHCG
                     LNLPEREALPVAGRFYCSEEHRRLAG"
     gene            680..2266
                     /locus_tag="VI319_09750"
     CDS             680..2266
                     /locus_tag="VI319_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY6864172.1"
                     /translation="MAGLALLSWPSAQAEAEGSFWTSLRYFNFYRIGVASVFLLAVLI
                     YGNELALGNHDLAAFRYTSMAYLGLAIAYHIVLRKSPAHFSFQLTLHVITDVVAITLL
                     MYASGGMRSGLGVMLLISLAAAAMVSRGRLMLFYAAIASIALLLEQSYWVLFQDTPTA
                     AFLQPGLMSIGYFATALITNQLAQRLIVNERVARQRGTDLANQMRINELVIQDVQDGV
                     LVVDANGLVRQHNRRVGALLEREPPERGQIDDYSDELARRLAAWRGGGGPASVSLRLP
                     ASGRLVRARFVPAGTAGGSVDLVFLEDLSKLQEQAQQLKLAALGRLTANIAHEIRNPL
                     SAMRHAGDLLVEESRSTQRDRLVQIIRDNTERLDRMVRDVLQLSRRDRVQPEQIRLRP
                     YLLAFVDEFAVKDSVPRECLVVEAELDASVDFDRVHLNQVLWNLLGNACRHSKRRPGS
                     VRVQVSQRANRVELHVIDDGEGVPRELQAQLFEPFFTTFASGTGLGLYIARELCAANG
                     ATLDYLDREQGADFRILWQT"
     gene            2263..3630
                     /locus_tag="VI319_09755"
     CDS             2263..3630
                     /locus_tag="VI319_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6864173.1"
                     /translation="MTNRRAERRSASSRQQGSVLVIDDEADIRELLEISLVRMGLAVD
                     CVGSVSAAKARLQDRQYDLCLTDMRLPDGDGLELVRHIGAHCNDLPVAVITAFGSTEN
                     AVAALKAGAFDYVTKPLSLEQLRALVKSALSLPQRPSLAKPGEPQLLGVSPAIEQVRS
                     MIEKLARSQAPIYITGESGSGKELAARLIHGKGARQDKAFVAVNCGAIPENLMESEFF
                     GYKKGAFTGAETDKDGFFQAASGGTLFLDEVADLPLGMQVKLLRAIQEKRVRKIGATA
                     EDPVDVRVMCATHQNLTARVESGKFRQDLYYRLAVIELKMPPLRECREDIAILAGAVL
                     DRLSAAGGRAAPRLSEAAQKALAAYDFPGNVRELENILERATALSGGQNIEPEDLQLA
                     VPGTVAEPAPNAKWPLQEYLDRIERDAILEALEKTRFNRTAAAKLLGITFRALRYRME
                     RLGIQ"
     gene            3672..4208
                     /gene="ampD"
                     /locus_tag="VI319_09760"
     CDS             3672..4208
                     /gene="ampD"
                     /locus_tag="VI319_09760"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEY6864174.1"
                     /translation="MEIGILREALQVPSPNWDLRPAGEALTLLVVHNISLPPGDFGGD
                     GIVRLFTNTLDCGAHPYYETLRGMRVSSHFLIRRGGELLQFVPCACRAWHAGASTWKG
                     RDRCNDFSIGVELEGADDVPYTDSQYRRLAELTATLKRLYPIADIVGHADIAPGRKTD
                     PGPAFDWARYRELLSRTA"
BASE COUNT          645 a         1480 c         1458 g          648 t
ORIGIN      
        1 tcgggctcga gcgcgagcgc ctcgaggtag cagcgcagcg cgtcgtcgtg ccggccctgg
       61 gcgtcgagca cattgccgag attgttttgc gccggagcgt tcgcgggatt gcgcgcgaga
      121 gccgcggaga tccgctccgc ggcctgcgcg aggtcgcccc gctggtacgc gagcacgccg
      181 agaaaatgcg cggcgtcgat gttcgcgggg tcggattcga ggatgcggcg atacagcgcg
      241 tcggcttccg ccaggcgccc tgcgcggtga tggccgagag cctcttcgat gctcggcgcc
      301 ccggcgaccg cggccgcagg ctcgctcgcc ctgcggtttc cgaaaagcca tcgaaacatc
      361 gggcggccgc cgtcagcggc gagcttcgct catcgagcca atcgtagcag atcgaccggt
      421 ataatgcgtc gcggaacgac gctttgcgga gcaccaacgc cttgagactg ctgctcattg
      481 ccctcgccgt gacgctcgtc tacgtgatcg tgcgcggcat gacgcgcgcg agcgcgccgc
      541 gccgcccgac gcggcccgtc gtcgagcgca tggtcccgtg cgcgcattgc ggcttgaacc
      601 tgcccgagcg cgaggcgctg cccgtcgccg gccgcttcta ctgcagcgaa gagcatcgcc
      661 ggctggcggg ctgagcgccg tggccgggct cgcgctcctt tcctggccgt ccgcgcaggc
      721 ggaggccgaa ggatcgttct ggacctcgct gcggtatttc aatttctacc gcatcggcgt
      781 cgcctccgtc ttcctgctcg ccgtcctgat ctacgggaac gagctggctc tcgggaacca
      841 cgacctcgcc gcgttccgat atacgagcat ggcgtacctc gggctcgcca tcgcgtatca
      901 catcgtcctg cgcaagagcc cggcgcactt cagcttccag ctcacgctcc acgtcatcac
      961 cgacgtcgtc gcgatcacgc tgctcatgta cgcgtcgggc ggaatgcgca gcggcctggg
     1021 cgtgatgctg ctcatctcgc tcgccgccgc cgccatggtg agccgcggac gcttgatgct
     1081 gttctacgcc gcgatcgcct cgatcgcgtt gctgctcgag cagtcgtact gggtcctatt
     1141 ccaggacacg ccgaccgcgg ccttcctcca gccgggactg atgtcgatcg gctacttcgc
     1201 gacggcgctg atcacgaacc agctcgcgca gcgcctgatc gtgaacgagc gggtcgcgcg
     1261 ccagcgcggc accgacctcg ccaaccagat gcggatcaac gagctcgtga tccaggacgt
     1321 ccaggacggc gtgctcgtag tcgatgccaa cggcctggtg cgccagcaca accgccgtgt
     1381 gggcgcgctg ctcgagcgcg agccgccgga gcgcgggcag atcgacgatt attcggacga
     1441 gctcgcgcgg cgcctcgccg cgtggcgcgg cggcgggggg ccggcgagcg tgagcctgcg
     1501 ccttccggcg agcgggcgcc tcgtgcgcgc gcgtttcgtc cccgcgggca ccgccggcgg
     1561 cagcgtcgac ctggtgttcc tcgaggacct gtcgaagctg caggagcagg cgcagcagct
     1621 caagctggcc gcgctcggcc gcctgacggc caacatcgcg cacgagatcc gcaatccgct
     1681 gtccgccatg cggcacgcgg gcgatctcct ggtcgaggaa tcgcgatcga cgcagcgcga
     1741 ccgcctcgtg cagatcatcc gcgacaacac cgagcggctc gaccgcatgg tgcgcgacgt
     1801 gctgcagctc agccgccgcg accgcgtcca gcccgagcag atacggctgc ggccgtatct
     1861 gctcgcgttc gtcgacgaat tcgcggtcaa ggacagcgtg ccgcgcgagt gcctggtggt
     1921 ggaagccgag ctggacgcct cggtcgattt cgaccgcgtg cacttgaacc aggtgctctg
     1981 gaacctgctc ggcaacgcct gccggcacag caaacggcgg ccgggcagcg tccgtgtgca
     2041 ggtctcgcag cgggccaatc gggtagaatt gcacgtcatc gacgacggcg agggcgtgcc
     2101 cagggagctg caagcgcagc tgttcgagcc gttcttcacc accttcgcga gcgggaccgg
     2161 cctcggactg tacatcgcgc gcgagctgtg cgcggcaaac ggcgccacgc tcgactatct
     2221 cgatcgcgaa cagggggcgg acttcaggat cctatggcag acgtgaccaa ccggcgagcg
     2281 gagcgcagga gcgcttcatc gcgtcagcag ggctcggtgc tggtcatcga cgacgaggcc
     2341 gatatccggg agctgctcga gatctcgctg gtacgcatgg gcctggcggt cgactgcgtc
     2401 ggctcggtgt ccgccgccaa ggcgcggctg caggaccgcc agtacgatct ttgcctgacc
     2461 gacatgcggc tgcccgacgg ggacggcctc gagctcgtgc gccacatcgg cgcgcattgc
     2521 aatgacctgc cggtcgcggt gatcacggcg ttcggcagca ccgagaacgc ggtggccgcg
     2581 ctgaaggcgg gcgcgttcga ctacgtcacc aagccgctgt cgctcgagca gctgcgcgcg
     2641 ctggtcaagt ccgcgctctc gctgccgcag cggccgtcgc tcgcgaaacc cggcgagccg
     2701 cagctcctcg gcgtgagccc ggccatcgag caggtgcgct cgatgatcga gaagcttgcc
     2761 cgcagccagg cgccgatcta catcacgggc gagtcgggca gcggcaagga gctcgcggcg
     2821 cggctcatcc acggcaaggg cgcgcgccag gacaaggctt tcgtggcggt gaactgcggg
     2881 gcgatccccg agaatctgat ggaaagcgag ttcttcggct acaagaaggg cgctttcacg
     2941 ggagccgaga cggacaagga cggctttttc caggccgcga gcggcggcac gctgttcctc
     3001 gacgaggtcg ccgacctgcc cctcgggatg caggtcaagc tcctccgcgc catccaggag
     3061 aagcgggtgc gcaagatcgg cgcgacggcc gaggaccccg tggacgtccg cgtcatgtgc
     3121 gcgacgcacc agaacctgac cgcgcgggtc gagtccggca aattccggca ggacctctac
     3181 tatcggctcg ccgtgatcga gctgaagatg ccgccgctgc gcgaatgccg cgaggacatc
     3241 gcgatcctcg cgggcgcggt cctggaccgg ctgtcggcgg ccggcggcag ggcggcgccg
     3301 cggctgtcgg aagccgcgca gaaggcgctt gccgcgtacg atttcccggg taacgtgcgc
     3361 gagctcgaga acatcctcga gcgcgcgacc gctctgtcgg gcgggcagaa catcgagccc
     3421 gaagatctcc agctcgcggt cccggggacg gtcgccgagc cggccccgaa cgcgaagtgg
     3481 ccgctccagg aatacctgga ccgcatcgag cgcgatgcca tcctggaggc gctcgaaaag
     3541 acccgcttca accgcaccgc ggcggcgaag ctgctcggga tcactttccg ggcgctgcgc
     3601 taccgcatgg agcggctggg catccaatag cgagcgcacg ctccgcccgc gtgagctcgc
     3661 gcgcaaccgc cgtggaaatc gggatcctgc gcgaggcgct ccaggtccca tcgccgaact
     3721 gggacctgag accggcgggc gaggccctga ccctgctcgt ggtccacaac atcagcctcc
     3781 ccccgggcga tttcggggga gacgggatcg tgcgcctgtt cacgaacacg ctggattgcg
     3841 gtgcccatcc gtactacgag acgctgcgcg gcatgcgcgt ctcgtcgcat ttcctgatcc
     3901 ggcgcggcgg cgagctgctc cagttcgtgc cctgcgcgtg ccgcgcctgg cacgcgggcg
     3961 cctcgacttg gaaggggcgc gatcgctgca acgacttctc gatcggggtc gagctcgagg
     4021 gcgccgacga cgtcccgtat acggactcgc agtaccgcag gctcgccgag ctcaccgcga
     4081 ccctcaagcg cctctatccg atcgccgaca tcgtcggcca cgccgacatc gcccccggac
     4141 gcaagaccga tcccgggccc gcgttcgact gggcccgcta ccgcgagctg ctgtcccgca
     4201 ccgcctgacg cccggtttcg gccggcgcgc g
//
LOCUS       DATDMW010000226         2682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_691958, whole genome shotgun sequence.
ACCESSION   DATDMW010000226 DATDMW010000000
VERSION     DATDMW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691958"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VI319_09765"
     CDS             complement(<1..647)
                     /locus_tag="VI319_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6864175.1"
                     /translation="MSLILGIHTGHESSAVLFDDATLIAAVSNERLSRIKNDGGKLSD
                     LAIDEVLRLAGRQRADVESLALLYTYFPEEYFVRETWLKELERRMHRARRRRANRERP
                     QMLLSNFIERLNARGKSFDAHFLRDRFLRDEGFARAKPRFFDHHDAHALAAAVYSGFR
                     DCAVVTMDGVGDENISHTSGTWRDGRYERLHVTDRLGASPGLFYSHITRLLGFQP"
     gene            complement(657..2549)
                     /gene="asnB"
                     /locus_tag="VI319_09770"
     CDS             complement(657..2549)
                     /gene="asnB"
                     /locus_tag="VI319_09770"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY6864176.1"
                     /translation="MCGIVGAVLAGGGDVRLDDAVACLEHRGPDQNATWREGEARLGF
                     RRLAIIDLSEAGHQPMHSPDGRYVVVFNGEIYNFQELRDDLEAKGERFRGHSDTEVLL
                     RLFAREGLERCLARLRGMFAFAVWDRHEKRLSLARDRLGVKPLVYAETPAAFLFGSEI
                     QSLFALDPGLSREPDFEGIDHYLTFQYVPAPRSGFAAVRKLPPAHAMTVRAGRVERVF
                     RYWDLDLTRKSALSFGEACEALREQVLEATKLRLVSDVPLGAFLSGGIDSSITVAAMA
                     RLGASPLRTFSIGFEDERFNELPHAREIATHLATEHHEMMVKADAVDVMPKMIEHLGE
                     PLADNSVMPTYYVSKFAKTGVTVALTGDGGDEVFAGYRRFYQIRRMEWLARSGLVPLW
                     RGIRKLAIAVENSTHPGRPPRRFPATRADESLGLDGVARYQHLLAFFSDEEKAGLVTP
                     YFRDRAGRVDPAAYLTAHFERPGDDVVNRYLYLDLSTYLPEDILFKVDVTSMASSLEC
                     RSPFLDHKLIEFAATLPGRYKLSAAGRHKHILKEAFAAWLPPGFMDRPKKGFSVPLAR
                     WLKQDLADMMRDTLVSRRTLARWFRQDVIERYVDEHVSGRASHTNRLWPLFVLALWAE
                     RFSVQV"
BASE COUNT          436 a          893 c          961 g          392 t
ORIGIN      
        1 atcggctgga acccgagcag gcgggtgatg tgcgagtaga agaggccggg cgacgcgccg
       61 agacgatcgg tgacgtgcag acgctcatag cggccgtcgc gccaggtgcc ggaagtgtgg
      121 ctgatgtttt cgtcgccgac gccgtccatg gtgacgacgg cgcaatcgcg gaaacccgag
      181 tacaccgcgg cggcgagcgc atgagcgtcg tgatggtcga agaagcgcgg cttcgcgcgc
      241 gcgaaaccct cgtcgcgcag gaacctgtcg cggaggaaat gggcgtcgaa cgacttgccg
      301 cgggcgttca ggcgttcgat gaagttcgac agcaacatct ggggccgctc gcggttcgcg
      361 cgccggcggc gcgctcggtg catgcggcgc tcaagctctt tcagccatgt ctcgcgcacg
      421 aaatattctt cgggaaaata ggtgtagagc agcgcgaggc tctcgacgtc ggcccgctgg
      481 cggccggcca gccgcagcac ttcgtcgatc gccaggtcgg acagcttgcc gccgtcgttc
      541 ttgatgcggg acaggcgctc attcgagacc gccgcaatca gcgtcgcatc atcgaacagg
      601 acggccgagg attcgtgtcc ggtatggatg ccgagaatca gtgacatggc ttcccctcaa
      661 acttggacgc tgaaccgctc ggcccacagc gccagcacga acaacggcca cagccggttc
      721 gtgtgcgagg cgcggcccga gacgtgctcg tcgacgtagc gctcgatcac gtcctggcgg
      781 aaccagcgcg cgagcgtcct acgcgagacg agcgtgtcgc gcatcatgtc ggcgaggtcc
      841 tgcttcagcc agcgcgcgag cggcaccgag aagcccttct tcggccggtc catgaacccc
      901 ggcggcagcc acgctgcgaa cgcctccttc aggatgtgct tgtgccggcc cgcggccgag
      961 agcttgtagc gcccgggcag tgtcgcggcg aactcgatca gcttgtgatc gaggaacggc
     1021 gagcggcact cgagcgagct cgccatgctg gtgacgtcga ccttgaacag gatgtcctcg
     1081 ggcaggtagg tgctcaggtc gagatagaga tagcggttca cgacgtcgtc gcccgggcgc
     1141 tcgaaatgcg cggtcaggta cgcggcggga tcgacgcgcc cggcacggtc acggaaatac
     1201 ggcgtcacca ggcccgcctt ctcctcgtcg gagaagaagg cgagcaggtg ctggtagcgc
     1261 gcgacgccgt cgagtccgag gctctcgtcg gcgcgcgtcg cggggaagcg ccgcggcgga
     1321 cggccggggt gcgtgctgtt ctcgacggcg atcgcaagct tgcggatccc gcgccacagc
     1381 ggcacgaggc ccgaacgcgc gagccactcc atgcgccgga tctggtagaa gcgccggtag
     1441 ccggcgaaca cctcgtcgcc gccgtcgccg gtcagtgcga ccgtcacgcc ggttttcgcg
     1501 aacttcgaga cgtagtacgt gggcatcacc gagttgtcgg cgagcggctc gccgaggtgc
     1561 tcgatcatct tgggcatcac gtcgaccgcg tcggccttga ccatcatctc gtggtgctcg
     1621 gtggcgaggt gcgtcgcgat ctcgcgcgcg tgcggcagct cgttgaagcg ctcgtcctcg
     1681 aagccgatcg agaaggtgcg caggggtgac gccccgaggc gcgccatcgc cgcgaccgtg
     1741 atcgacgagt cgatgccgcc ggaaaggaac gcgccgagcg gcacgtcgct caccaggcgc
     1801 agcttggtcg cctcgagcac ctgctcgcgc agcgcctcgc aggcctcgcc gaaggagagc
     1861 gcgctcttcc gggtgaggtc caggtcccag tagcggaaca cgcgctcgac gcggccggcg
     1921 cgcacggtca tcgcgtgcgc gggcgggagc ttcctcaccg cggcgaagcc cgagcgcggc
     1981 gcggggacgt actggaaggt gaggtaatgg tcgatgccct cgaagtcggg ctcgcgagag
     2041 agccccggat cgagcgcgaa caacgactgg atctccgagc cgaagaggaa tgccgccgga
     2101 gtctcggcgt acacgagcgg cttcacgccg aggcggtcgc gcgcgagcga gaggcgcttc
     2161 tcgtgccggt cccacaccgc gaacgcgaac atcccgcgca gccgcgcgag gcagcgctcc
     2221 aggccctcgc gcgcgaacag gcgcagcagc acctcggtgt cggagtggcc gcggaagcgc
     2281 tcgcccttcg cctcgagatc gtcgcgcagc tcctggaagt tatagatctc gccgttgaac
     2341 acgacgacgt agcggccgtc gggcgaatgc atcggctggt ggccggcctc ggagagatcg
     2401 atgatggcga gccggcggaa ccccaggcgc gcctcgccct cgcgccacgt cgcgttctgg
     2461 tccgggcccc ggtgctcgag gcacgcgacg gcgtcgtcga gccgcacgtc gccgccgccg
     2521 gcgagcaccg cgccgacgat gccgcacatc agccgcctcc catgaggatc gactcgagcc
     2581 tggcggcgtt cgcggcccag gtgaattccg atcgcgcgcg cgccctcgcg ctcgcgccga
     2641 gcgtggcgag gcccgcacgg tcggcgtcgg cggccgcgat cg
//
LOCUS       DATDMW010000227         2443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1314543, whole genome shotgun sequence.
ACCESSION   DATDMW010000227 DATDMW010000000
VERSION     DATDMW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314543"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VI319_09775"
     CDS             complement(<1..290)
                     /locus_tag="VI319_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY6864177.1"
                     /translation="MSDASDPIALSIDPAAETERICGVLRQQVLRLLRRRGVVLGLSG
                     GVDSSVVAALCVRAFGPTAVFGVLMPEQDSDPDSLRLGRTVASWLGIDIAIE"
     gene            complement(287..1792)
                     /locus_tag="VI319_09780"
     CDS             complement(287..1792)
                     /locus_tag="VI319_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6864178.1"
                     /translation="MRVERFLWEAAERFPDKTAVVAGDARPTYAELAGRAAGLARELK
                     RRGLRRGDRVALFLHNSVEAVAGVFGVLAAGGVFSVVNPGTKADKLAYILNNARASAI
                     ITEPRLAAVAKEAAALAPSILDLIETPFDFPADDARPVGGIDLDLAMIVYTSGSTGFP
                     KGVMMTHANIDAAATSITTYLESRADDVVLSVLPMAFDYGLYQALMCAKVGATLVLEK
                     SFTYPAVVLEKLRAEKVSGLPLVPTLAAMLLQMKQLEPGLFPHLRYLTNTAAALPPAH
                     IARLRQIFPQARVYSMYGVTECKRCTWLPPEQLDVRPSSVGIPIPGTEAWIVDETGER
                     VPPGTTGELVIRGAHVMKGYWENEEATRRALRPGPYPWENVLYTGDLFKADEEGYLYF
                     VARMDDIIKTRGEKVSPREVENCLYEIPGVREAAAIGVPDAVLGNAIKAVIAADPGAG
                     LTERDIIRHCAARLEDFMVPKHVEFRGELPKSENGKIARREIASEAAEVIA"
     gene            complement(1797..2072)
                     /locus_tag="VI319_09785"
     CDS             complement(1797..2072)
                     /locus_tag="VI319_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY6864179.1"
                     /translation="MNHDITQAVRGFIAENFLFRDEADAIASDASLLEAGIIDSTGVL
                     ELVSFLETTFGIEVADEEMLPENLDSIRAITGYVSRKLSERRIELVA"
     gene            complement(2147..>2443)
                     /locus_tag="VI319_09790"
     CDS             complement(2147..>2443)
                     /locus_tag="VI319_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY6864180.1"
                     /translation="LRRALGRHLPEAIVERPKQPYRAPDAESFAGPDAPEYVRALLSP
                     QAVARAGYFEPRAVAHLAAKCARGQPPVSAGDNMAFMAVLSTQLLHARFVGALI"
BASE COUNT          376 a          836 c          862 g          369 t
ORIGIN      
        1 tcctcgatcg cgatgtcgat gccgagccag ctcgccaccg tgcggccgag gcgcaggctg
       61 tcgggatcgg aatcctgctc gggcatcagc acgccgaaca ccgccgtcgg gccgaaggcg
      121 cgcacgcaaa gcgcggcgac cacgctcgag tcgacgccgc ccgagagccc gagcaccacg
      181 ccgcgccggc gcaggagccg cagcacctgc tggcgcagca cgccgcagat gcgctcggtc
      241 tcggcggccg gatcgatcga gagcgcgatc gggtcggacg cgtcgctcat gcgatcacct
      301 ctgccgcttc gctcgcgatc tcccgccgcg cgatcttgcc gttctcgctc ttcggcagct
      361 cgccgcggaa ctcgacgtgc ttgggcacca tgaaatcctc cagccgcgcg gcgcaatggc
      421 gaatgatgtc ccgctcggtg agcccggcgc cgggatcggc ggcgatcact gccttgatcg
      481 cgttgccgag caccgcgtcg ggaacgccga tcgccgcggc ctcgcgaacg cccgggatct
      541 cgtacaggca gttctccacc tcgcgcgggc tcaccttctc gccgcgcgtc ttgatgatgt
      601 cgtccatgcg cgcgacgaag tagaggtagc cttcctcgtc ggccttgaag aggtcgccgg
      661 tgtagagcac gttctcccac gggtacggcc ccgggcgaag cgcgcggcgc gtcgcctcct
      721 cgttctccca gtagcccttc atgacgtgcg cgccgcggat caccagctcg ccggtcgtgc
      781 cgggcggcac gcgctcgccc gtctcgtcga cgatccacgc ctccgtgccg ggaatcggga
      841 tgccgacgga ggaggggcgc acgtcgagct gctcgggcgg cagccacgtg cagcgcttgc
      901 actcggtcac gccgtacatc gagtagaccc gcgcctgagg gaagatctgg cgcaggcggg
      961 cgatgtgggc cggcggcagc gcggcggcgg tattggtcag gtagcgcagg tgagggaaga
     1021 ggcccggctc gagctgcttc atctgcagca gcattgccgc cagcgtcggc acgaggggca
     1081 ggccgctgac cttctccgca cgcagcttct ccagcacgac cgcgggatag gtgaacgact
     1141 tctcgagcac gagcgtcgcg ccgaccttgg cgcacatcag ggcctggtag agcccgtagt
     1201 cgaacgccat cggcagcacc gagaggacga cgtcgtcggc gcggctctcc aggtaagtgg
     1261 tgatcgacgt cgccgcggcg tcgatgttcg cgtgcgtcat catcacgccc ttcgggaagc
     1321 cggtcgatcc cgaggtgtag acgatcatcg ccaggtcgag gtcgatgccg ccgaccggcc
     1381 gtgcgtcatc tgccggaaaa tcaaacgggg tttcgatcaa gtccaggatc gaaggagcga
     1441 gggccgccgc ttccttcgcg acggcggcga gccgcggctc cgtgatgatc gccgaggcac
     1501 gcgcgttgtt caggatgtag gcgagcttgt cggccttggt gcccgggttc acgacgctga
     1561 atacgccgcc cgcggcgagc acgccgaaca cgccggcgac cgcctcgacc gagttgtgca
     1621 ggaagagggc gacgcggtcg ccgcggcgca ggccgcggcg cttcagctcg cgcgcaagcc
     1681 ctgcggcgcg gccggcgagc tcggcatagg tcggccgcgc gtctccggcg acgacggcgg
     1741 tcttgtcggg gaagcgctcc gccgcctccc acaggaaccg ttcgacgcgc acggattcag
     1801 gcgaccagct cgatgcgccg ctcgctcagc ttgcggctga cgtagcccgt gatcgcacgg
     1861 atggagtcga ggttctccgg cagcatctcc tcgtcggcga cctcgatgcc gaaggtggtc
     1921 tcgaggaacg agaccagctc gagcacgccg gtcgagtcga tgatccccgc ctcgaggagc
     1981 gaggcgtcgc tcgcgatcgc gtcagcctcg tcgcgaaaca ggaaattctc tgcgatgaaa
     2041 ccgcgtacgg cctgggtgat gtcgtggttc attttccatc cgggtgggta gcggagatga
     2101 cgatagcgga ccttcgtgac accggattca cgcagcggcg cgcctttcag atcaaagcgc
     2161 cgacgaagcg cgcgtgcagg agctgcgtcg agagcacggc catgaaggcc atgttgtcgc
     2221 cggcggaaac gggcggctgc ccgcgagcgc acttcgccgc cagatgggct acggcgcgcg
     2281 gctcgaaata gcccgcgcgg gcgacggctt gcggcgacag cagcgcgcgc acgtactccg
     2341 gcgcgtccgg cccggcgaag ctctcggcgt cgggagcccg gtagggctgc tttggacgct
     2401 cgacgatcgc ctccggcagg tggcgcccga gcgcgcggcg cag
//
LOCUS       DATDMW010000228         5172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2312135, whole genome shotgun sequence.
ACCESSION   DATDMW010000228 DATDMW010000000
VERSION     DATDMW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312135"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..2796
                     /gene="ppc"
                     /locus_tag="VI319_09795"
     CDS             37..2796
                     /gene="ppc"
                     /locus_tag="VI319_09795"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY6864181.1"
                     /translation="MDAVSPAPEEKELLLREDRRLLGRLLGDVIAEQVGPRTRDLVES
                     IRQTAVRFRRSLSDPALAGEAQGVKAELEAQLDALPIEETLHVVRAFSYFSLLLNIAE
                     DAHQHRRRQAHAQSGSPPRPGSFGYALERAAEGRTASDLLAWFARARVSPVLTAHPTE
                     VQRQSILECEREIARLILLPATPARDRALRAEVLRLWMTAMLRLTKLAVADEIANALA
                     YFRLTFIPEIPRLYADLEEALRARYALEREPWLAPFLTVGSWVGGDRDGNPNVGAHTL
                     EIAVSQQARMILAHYMEEVNQLGRELGLSQRLMPTPREVLELAARSGDDADQRADEPY
                     RRILSGIYSRLAATAKTLTGLEATPAPRFERAPYESAAEFAADLDLIARALDAQGAAL
                     LAEGRLRALRRKVGVFGFHLAAIDLRQSSDAHAQAVAELLARVGVETGYEALDEPART
                     ALLARELAGPRPLRSPHFTYSAPLERELGIVDAAAQAQRDLGPAAVSRYIISHCESVS
                     DLLEVGVLLREAGLLRPHDRSAEVDVDVIPLFESLADLERCGEIMRQAVENPAYRAWV
                     RARGDEQEVMLGYSDSNKDGGYLSSSWTLYKATGDLMRVCRAHGVRLRLFHGRGGTVG
                     RGGGPSYEAVLSQPPGSVDGALRLTEQGEVIASKYADPENGRHNLEILAAATLEASFM
                     TQSADGHGRHAEIMEALSSLALAAYRGLVHDTPGFMDYFRQATPFAEIAKLNIGSRPA
                     SRKASQRLEDLRAIPWVFSWSQCRVMLPGWYGFGAAVSQWLETKRGTLAELREMHERW
                     PFFRAVLSNLDMVLAKTDLGIGERYAELADAGVRAAVFPRIRAEWHAARERLFAITGA
                     TGFLADNPTLARSIRNRFAYLDALNHIQLALLRRYRGGDTEVRTVRAIHLTINGIASV
                     LRNSG"
     gene            complement(2812..3183)
                     /locus_tag="VI319_09800"
     CDS             complement(2812..3183)
                     /locus_tag="VI319_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6864182.1"
                     /translation="MNDRQLTRDGAQPAPAIAPRVDVIEDATGISILADLPGVSKERL
                     SLRIEGETLQIEGDVASDAPEGLEAVYAEVQAPRYRRAFTLSRELDTDKIDAKLKDGV
                     LTLRIPKAEHAQPRRIQVQAG"
     gene            complement(3213..3662)
                     /locus_tag="VI319_09805"
     CDS             complement(3213..3662)
                     /locus_tag="VI319_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6864183.1"
                     /translation="MYESLLTVPGWLAELDRMQRDVQQALGTLGLPSSIRAVAQGAFP
                     QINIGATPREIVVYAFAPGLDPARIDVTVDRGVLTLSGHRLDTRPGQDAKRSVYAAER
                     FSGAFRRAISLPEDADPARVSAQYRDGVLRISIARRESARPKRVEIQ"
     gene            3853..5127
                     /locus_tag="VI319_09810"
     CDS             3853..5127
                     /locus_tag="VI319_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY6864184.1"
                     /translation="MPSAPPTHPLDAAADLAALQVLHARVSRLLSVRTLGDAMQEILR
                     AAIDMQGTELGNVQVLNPATGKLEIVAQRGFRAPFLEHFREVAVDDDSACARALRSGR
                     RHAIHDIRADPECGAEYRRVAAEAGYRGVQSTPLVGRDGSLLGMLSTHYPEPRHLSER
                     ESRALDLYARQAADIIERIRREQALEAADRRKDEFIAVLSHELRNPLAPIVNGVRILR
                     QGARDPAAAERVLAMIERNALHLARMVDDLLDAARIAEGKLSLHLECVDLAALVRQHI
                     EAMIPLVAAGGHALELDLPREPLTVECDPARIAQVLDNLLLNAGKYTPAGGRIEVSLK
                     RVGQGACFSVRDNGIGIPAELLPKLFGMFVQGRHPEGRGGLGIGLALAKRLVELHGGT
                     ITARSPGSGAGAEFVVELPAHDSVTPASRPMP"
BASE COUNT          698 a         1920 c         1858 g          696 t
ORIGIN      
        1 gctccccgag cgagccgcgc ggggcataat ctccgcgtgg acgccgtttc acccgcaccg
       61 gaagaaaagg agctcctcct ccgcgaagac cgccgtctcc tcggccgcct gctcggcgac
      121 gtgatcgcgg agcaggtcgg gccgcggacg cgcgatctgg tcgagtcgat ccggcagacg
      181 gcggtgcggt tccggcgctc gctctcggat ccggcgctcg cgggcgaggc tcaaggcgtg
      241 aaggcggagc tcgaggcgca gctcgacgcg ctgccgatcg aggagacgct gcacgtggtg
      301 cgggcgttca gctatttctc gctcctgctc aacatcgcgg aggacgcgca ccagcaccgc
      361 cggcggcagg cgcacgcgca aagcggctcg ccgccccggc cggggagctt cgggtatgcg
      421 ctcgagcgcg cggcggaggg gaggacggcg agcgatcttc tcgcgtggtt cgcgcgggcg
      481 cgggtgagcc cggtgctgac ggcgcatccg acggaggtgc agcgccagag cattctcgag
      541 tgcgagcgcg agatcgcgcg gctgatcctg ctgccggcga cgcccgcgcg cgaccgggcg
      601 ctgcgcgcgg aggtgctgcg gctgtggatg acggcgatgc tgcgcctgac gaagctcgcg
      661 gtcgcggacg agatcgcgaa cgcgctcgcg tatttccggc tgacgttcat cccggagatc
      721 ccgcggctgt acgcggacct ggaggaggcg ctgcgcgcgc gctatgcgct cgagcgcgag
      781 ccgtggctcg cgcccttcct cacggtcggc agctgggtcg gcggcgaccg cgacggcaat
      841 ccgaacgtgg gcgcgcacac gctcgagatc gcggtgtcgc agcaggcgcg catgatcctc
      901 gcgcattaca tggaggaggt gaaccagctc ggacgcgagc tcggtctctc gcagcggctg
      961 atgcccacgc cgcgcgaggt gctcgagctc gccgcgcgct cgggcgacga cgccgatcag
     1021 cgcgccgacg agccgtaccg gcgcatcctc tcgggcatct acagccggct cgcggccacg
     1081 gcgaagacgc tcacgggcct ggaggcgacg cccgcgccgc gcttcgagcg ggcgccttac
     1141 gagagcgccg ccgaattcgc ggccgacctc gacctgatcg cgcgcgccct ggacgcgcag
     1201 ggcgcggcgc tcctcgccga aggacggctg cgcgcgctgc ggcgcaaagt cggcgtcttc
     1261 ggcttccacc tcgccgcgat cgacctgcgc cagagctccg acgcgcacgc gcaagcggtc
     1321 gccgagctcc tcgcgcgggt cggcgtcgag accggctacg aagcgctgga cgagccggcg
     1381 cgcacggcgc tcctcgcgcg cgagctcgcg ggcccgcggc cgctgcgctc gccgcacttc
     1441 acctattccg cgccgctcga gcgcgagctc ggcatcgtcg acgccgcggc gcaggcgcag
     1501 cgggacctcg ggcccgcggc cgtgtcgcga tacatcatct cgcactgcga gtcggtgtcc
     1561 gacctgctcg aggtcggcgt gctcctgcgc gaggccgggc tgctgcgtcc ccacgatcgg
     1621 agtgcagagg tcgacgtcga cgtcatcccg ctgttcgagt cgctcgccga tctcgagcgc
     1681 tgcggcgaga tcatgcgcca ggcggtcgag aaccccgcgt accgcgcctg ggtgcgcgcc
     1741 cgcggcgacg agcaggaggt gatgctcggc tactccgact cgaacaagga cggcggctac
     1801 ctctcctcca gctggaccct gtacaaggcg accggcgacc tgatgcgcgt gtgccgcgcg
     1861 cacggcgtgc gcctgcggct cttccacggc cgcggcggca cggtcggccg cggcggcggc
     1921 ccgagctacg aggcggtgct ctcgcagccg ccgggcagcg tcgacggcgc gctgcggctc
     1981 accgagcagg gcgaggtcat cgcgagcaag tacgccgatc ccgagaacgg ccggcacaac
     2041 ctcgagatcc tggccgcggc gacgctcgag gcgagcttca tgacgcagtc ggccgacggc
     2101 catggccgcc acgccgagat catggaagcg ctctcgtcgc tcgcgctcgc cgcctaccgc
     2161 ggcctcgtcc acgacacgcc cggcttcatg gactacttcc gccaggcgac gccgttcgcc
     2221 gagatcgcga agctcaacat cggcagccgc ccggcgtcgc gaaaagcctc gcagcgcctg
     2281 gaagacctgc gcgcgatccc ctgggtgttc agctggagcc agtgccgggt catgctgccg
     2341 gggtggtacg gcttcggcgc cgccgtctcg caatggctgg agacgaagcg cggcacgctc
     2401 gcggagctgc gcgagatgca cgagcgctgg ccgttcttcc gcgcggtgct ctccaacctc
     2461 gacatggtgc tcgccaagac cgacctcggc atcggcgaac gctacgccga gctcgccgac
     2521 gccggcgtgc gcgcggcggt gttcccgcgc atccgggccg agtggcacgc ggcgcgcgag
     2581 cggctcttcg cgatcaccgg ggcgaccggc ttcctcgcgg acaacccgac gctcgcgcgc
     2641 tcgatccgaa accgcttcgc ctacctggac gcgctcaacc acatccagct cgcgctcctg
     2701 cggcgctacc gcggcggcga caccgaggtg cgcaccgtgc gcgccatcca cctcaccatc
     2761 aacggcatcg cctcggtcct gcgcaacagc ggctagcgcg acaggacggt ctcagcccgc
     2821 ctgcacctgg atccgccgcg gctgcgcgtg ctccgccttc gggatgcgca gggtcagcac
     2881 gccgtccttc agcttcgcgt cgatcttgtc cgtgtcgagc tcgcggctga gcgtgaaggc
     2941 ccggcggtac cgcggcgcct gcacctccgc gtagacggct tccaggccct ccggcgcgtc
     3001 ggaggcgacg tcgccctcga tctgcagcgt ctcgccctcg atgcgcagcg aaagccgctc
     3061 cttcgacacg ccgggcaggt cggcgaggat gctgatgccc gtcgcgtcct cgatcacgtc
     3121 gacgcgcggc gcaatcgcgg gcgcgggctg cgccccgtcg cgcgtgagct gtcggtcgtt
     3181 catggcggtg gctcctctcg gatggtggga tttcactgga tctcgacgcg cttcggccgc
     3241 gccgactcgc gccgggcgat gctgatgcgc agcacgccgt cgcggtactg cgccgacacg
     3301 cgcgcgggat cggcgtcctc gggcaggctg atcgcgcgcc ggaacgcgcc cgagaagcgc
     3361 tcggcggcgt agacgctgcg cttggcatcc tgcccggggc gcgtgtcgag gcgatgcccc
     3421 gacagggtca gcacgccgcg gtccacggtc acgtcgatcc tggcgggatc gaggccgggc
     3481 gcgaaggcgt agacgacgat ctcccggggc gtcgccccga tgttgatctg cggaaacgcg
     3541 ccctgggcca cggcgcggat gctgctcggc agccccagcg tccccagcgc ctgctgcacg
     3601 tcgcgctgca tgcggtcgag ctcggcgagc cagccgggga cggtcaacaa cgactcgtac
     3661 atggcggtcc tcctgtcctg ggcaaagcgt gcgggaaagg catcccgtcc gcctgggcgc
     3721 gagattgagg gcgggcaccg cgcatttcaa gggcggcgaa gtccgggcgc cccggcgggg
     3781 aacgcccccc gcttcgcggg ggtcataagc cgaacggggg acatcgtccg tccccggcac
     3841 aggtgccgcc ccatgccgtc cgctccgccg acgcacccgc tcgacgccgc cgccgacctc
     3901 gccgcgctgc aggtgctgca cgcgcgcgtc agccgcctgc tctcggtgcg tacgctgggc
     3961 gacgcgatgc aggagatcct gcgcgcggcg atcgacatgc agggcaccga gctcggcaac
     4021 gtccaggtcc tcaatcccgc gaccggcaag ctcgagatcg tggctcagcg cggcttccgc
     4081 gcgccgttcc tcgagcactt ccgcgaggtc gccgtggacg acgattccgc gtgcgcgcgg
     4141 gcgctgcgca gcggccggcg ccacgccatc cacgacatcc gggccgatcc cgagtgcggg
     4201 gccgagtacc ggcgcgtcgc cgccgaggcg ggataccggg gggtgcagtc gacgccgctc
     4261 gtgggccggg acggcagcct gctcggcatg ctctccaccc attatcccga gccgcgccac
     4321 ctctccgagc gcgagtcgcg ggcgctcgac ctctacgcgc gccaggcggc cgacatcatc
     4381 gagcgcatcc ggcgcgagca ggcgctcgag gcggccgacc ggcgcaagga cgaattcatc
     4441 gcggtcctgt cgcacgagct gcgcaacccg ctcgcgccga tcgtgaacgg cgtgcgcatc
     4501 ctgcgccagg gcgcgcgcga ccccgccgcc gccgagcgcg tgctggcgat gatcgagcgc
     4561 aacgccctgc acctcgcgcg catggtcgac gacctgctcg acgccgcgcg catcgccgag
     4621 ggcaagctct cgctgcacct ggaatgcgtc gatctcgccg cgctggtgcg ccagcacatc
     4681 gaggccatga tcccgctcgt cgcggcgggc ggccacgcgc tcgagctcga tctcccgcgc
     4741 gagccgctga cggtcgaatg cgacccggcg cgcatcgcgc aggtcctcga caacctcctg
     4801 ctcaacgcgg gcaagtacac gccggccggc ggccgcatcg aggtgagcct gaagcgcgtg
     4861 ggacagggcg cctgcttcag cgtgcgcgac aacggcatcg gcattccggc cgagctcctg
     4921 ccgaagctct tcgggatgtt cgtgcagggc cggcaccccg aggggcgggg cggcctcggc
     4981 atcggcctcg cgctagcgaa gcgcctggtc gagctgcacg gcggcaccat caccgcgcgc
     5041 agccccggct cgggcgccgg cgccgagttc gtcgtcgagc tgcccgctca cgacagcgtc
     5101 acgcccgcga gccggccgat gccgtaggtg agggcggccg cggcgaggcc gagcagcagc
     5161 tgccgcgccg cc
//
LOCUS       DATDMW010000229         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1564302, whole genome shotgun sequence.
ACCESSION   DATDMW010000229 DATDMW010000000
VERSION     DATDMW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564302"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2066)
                     /gene="hrpA"
                     /locus_tag="VI319_09815"
     CDS             complement(<1..2066)
                     /gene="hrpA"
                     /locus_tag="VI319_09815"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEY6864185.1"
                     /translation="MTRFSAEELERRRASAPRPEFPPELPISARRAEIAAALRDHPAI
                     IVSGETGSGKTTQLPKICLQAGRGVAGMIGHTQPRRIAARATAARIAQELGAETGGAV
                     GYKIRFTDKVGPRTYVKLMTDGILLAETQGDRDLLQYDTLIIDEAHERSLNIDFLLGY
                     VRGLLPRRPDLKLIVTSATLDAERFSRQFAFDGRAAPVIEVSGRLYPVEIRYRPVGED
                     DDERDLEQAIADAVDELALAGPGDVLVFLPGEREIREAAETLRKHHPAHTEILPLYAR
                     LSAAEQERVFKPHPGRRIVLATNVAETSLTVPGVRYVVDAGLARVKRYSYRNKVEQLR
                     VEPVSQASAKQRAGRCGRIANGICIRLYAEDDHDSRPAFTDPEVLRSSLAGVILRMKA
                     LGLGDVEAFPFIDPPAPKAIQDGYALLSELGAVGDDRELTETGRQLARLPLDPRIGRM
                     ILAALSENALEEVLIIAAALSVQDPRERPLERAPAADEAHKRFEDEKSDFLSYLKLWK
                     FFEDALAHRKSGRKLHQACRDHFLSFSRMREWRDVHAQLKELVAEGGWRFSEKPATYA
                     QVHRALLAGLLGNVGMKTEEGNYLGARGIKFWIHPGSGVGRKAGRWIVAAELAETTRL
                     YARCVATIDPDWLESIGAHLVKRHQVDPHWEKTPARVAAFERGTLYGLPLYTNRRVHY
                     GPMDPV"
BASE COUNT          328 a          727 c          764 g          334 t
ORIGIN      
        1 tcgaccgggt ccatgggacc gtagtgcacg cggcgattcg tgtacagcgg caggccgtac
       61 agcgtgccgc gctcgaacgc cgcgacccgc gccggcgtct tctcccagtg cgggtcgacc
      121 tggtggcgct tcacgaggtg ggcgccgatc gattccagcc agtccgggtc gatggtcgcg
      181 acgcaacgcg cgtagagacg cgtcgtctcg gccagctcgg cggccacgat ccagcgccct
      241 gccttccggc cgacgcccga ccccgggtgg atccagaatt tgatcccgcg ggcgccgagg
      301 taattgccct cctcggtctt catgccgacg ttcccgagaa ggccggccag gagcgcgcga
      361 tggacctgcg cgtaagtcgc cggcttctcc gagaagcgcc atccgccctc ggcgaccagc
      421 tccttgagct gcgcgtggac gtcgcgccac tcgcgcatac ggctgaacga caggaagtga
      481 tcccggcagg cctggtgcag cttgcgcccc gacttgcggt gagcgagcgc atcctcgaag
      541 aacttccaga gcttgaggta ggacaggaaa tccgacttct cgtcctcgaa gcgcttgtgc
      601 gcctcgtccg ccgcgggggc gcgctcgagc ggccgctcgc gcggatcctg caccgacagc
      661 gcggcggcga tgatcagcac ctcctcgagg gcgttctccg agagggctgc gaggatcatg
      721 cggccgatcc gcggatcgag cggaagccgc gcgagctggc gtccggtctc cgtgagctca
      781 cggtcgtcgc cgacggctcc cagctcggag agcagcgcgt agccgtcctg gatcgccttc
      841 ggcgcgggcg gatcgatgaa aggaaaggcc tcgacgtctc cgaggccgag cgctttcatg
      901 cgcaggatca cgccggcgag cgacgagcgc agcacctccg gatcggtgaa ggcgggccgc
      961 gagtcgtggt cgtcctcggc gtagaggcgg atgcagatgc cgttcgcgat gcggccgcag
     1021 cgtcccgcgc gctgcttcgc cgacgcctgc gataccggct cgacgcgcag ctgctcgacc
     1081 ttgttgcggt agctgtagcg cttcacccgc gcgagccccg cgtcgacgac gtagcgcaca
     1141 ccgggcacgg tgagcgaagt ctcggcgacg ttggtcgcga gaacgatgcg gcgtcccggg
     1201 tgcggcttga atacgcgttc ctgctcggcg gccgacaggc gcgcgtagag cggaaggatc
     1261 tccgtgtgcg caggatgatg cttgcgcaac gtctcggcgg cttcgcggat ctcgcgctcg
     1321 cccgggagaa acacgagcac gtcgcccgga ccggccagcg caagctcgtc caccgcgtcc
     1381 gcaatcgcct gctcgaggtc gcgctcgtcg tcgtcctcgc cgaccgggcg ataccggatc
     1441 tcgaccgggt acaggcgccc ggagacctcg atcaccggtg ccgccctgcc atcgaaagcg
     1501 aactgccgcg agaagcgctc ggcatcgagc gtcgccgagg tgacgatcag cttgagatcg
     1561 ggccgccgcg gaagcagccc acgcacgtac ccgagcagga aatcgatgtt gaggctgcgc
     1621 tcgtgcgcct cgtcgatgat gagcgtgtcg tactggagca gatcgcggtc gccctgggtc
     1681 tcggcgagca ggatgccgtc ggtcatgagc ttcacgtagg tccgcggacc gaccttgtcg
     1741 gtgaaccgga tcttgtagcc gaccgctccg cccgtctcgg ccccgagctc ctgcgcgatg
     1801 cgcgcggcgg tggcccgcgc ggcgatgcgc cgcggctggg tgtgcccgat catgcccgcg
     1861 acaccgcgac ccgcctggag gcagatcttg gggagctgcg tcgtcttccc ggagccggtc
     1921 tcgccgctga cgatgattgc cgggtggtcg cgcagggcgg cggcgatctc cgcgcgcctg
     1981 gcggagatcg gcagctcggg agggaactcg ggacgtggag cacttgcgcg tcggcgctcg
     2041 agctcctccg cagagaatcg tgtcattcgg cgtcggggcg gacgatgctc ccgaagtcta
     2101 ccaaggccgg gagggtcgcc cctgagatga cggggcgaaa aaaaaaaagg tac
//
LOCUS       DATDMW010000230        11666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_692669, whole genome shotgun sequence.
ACCESSION   DATDMW010000230 DATDMW010000000
VERSION     DATDMW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692669"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VI319_09820"
     CDS             <1..462
                     /locus_tag="VI319_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864186.1"
                     /translation="VDLVPAFHLEGLREIVYLPEAAPDPVPSLPSSRFACSRPRAEFL
                     QEERRVYVYDFDSPDLFFHMLLHEIGHFVFFLVIGSRVKKHWVMHLYPGSSCITPYAE
                     TSAIEDFAETYAYYVLDPDALARAHAVKHAFMRDFVFSGDPDTLKERARGA"
     gene            472..2511
                     /locus_tag="VI319_09825"
     CDS             472..2511
                     /locus_tag="VI319_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY6864187.1"
                     /translation="MFVAIDIGGTFTDLMAFDEASGAFSQAKSLTTPAQLVQGIIDCL
                     KKSAVDPGAIGELIHGSTTAINTLIERKGAKTGLIVTRGTRDVYAIGRGNRPEAYDLF
                     FHRHRPLVPRHLTREVGGRLLASGAEHEPLDRAGVEDACRRLAAEGVEAVAVCFLHSY
                     ANPEHEREAGAIVRRLLPGAYVSLSHEILREYREYERTSTTVVNAYVGPKVGGYVQSL
                     ARGLGGIGFRGDLTIMQSNGGVMTPEVATGRPVAMMESGPVGGIIAAARVAAALGHAD
                     AISFDMGGTTAKASLIREGQPATASGYHVGGYASGHPVMVPVIDVIEVGAGGGSIAWI
                     DDVGALKVGPRSAGADPGPVCYGAGGTEPTITDANLVLGRLDPDDFLGGQMVLDAKGA
                     ERALQEKIARPLGLSSVAAAQAIVDIAVSRMSLAVREVSVAKGHDPRDFALVASGGAG
                     PLHVVAVARELAIPTVIVPRFPAHFSALGMLAADERHDVARTFYADLARVDFAALRAA
                     LDEMRREGLAGLRNRRSIEERLQLDLRYVGQEFTLPVPATEAQLADADRAAIRAAFDA
                     LYEQRYAHSSPREPVEIVNVRLAVIGKRPRMEFPKLREGTKAEPASVQEVFFPGSERP
                     VRCAVYERESLPAGARFAGPALVREHGTTTVMPPATECRVAATGELVIAEMGSAR"
     gene            2508..4262
                     /locus_tag="VI319_09830"
     CDS             2508..4262
                     /locus_tag="VI319_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY6864188.1"
                     /translation="MKDLDPVTLEVVRNALPAIANEMAADLQRTSYNMMIYEVRDFCT
                     ALLDVDGALISQNVGGVSHFVADLGVVVADGVMRYGREGFAPGDVIITNHQAVAGQHL
                     NNVVIYVPCFFEGELLAFAMVRAHWIDVGGRSTGFGAGPDVPDPWQEGLQLDQLKIHE
                     AGKLDETLLRVIRDNIRFPESSLGDMNSQIAACRLGARRLDELFRRYGRATLLAAIRR
                     IFEETERKCRNVVAQLADGEYEAESFFDDDGVTKGERVRIHAKVTVDKGAMTIDLSGC
                     SPERKAAVNSRTLAAARVAYKALTGPLEPVNEGSFRALEVIIPEGSIMMARYPAPMAG
                     WSLFVPMVVETIVTSLARAMPERIPAAHHALLGGAVVFFGTNPETGRRFVVQSVEGGG
                     WGGRPFEDGESATVSVCQGDVRNGSIEGIEMKCPVLIEERALRPDSGGAGKWRGGLGV
                     SLRVHNFVEGRWNFERASRAGCPPWGLWGGRAGASGGYWLSKPGDAEPRGMFGSQHAV
                     PRDCTVIVRTGGGGGWGDPLERDPKRVREDAIEELVSIDAAREHYGVVLGPGFEIDVE
                     ATAALRAKQSRKKQRQPA"
     gene            4259..5476
                     /locus_tag="VI319_09835"
     CDS             4259..5476
                     /locus_tag="VI319_09835"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY6864189.1"
                     /translation="MTALERFGEWIAGAAIPTEGVALHLVDAVAAWIAGAGTAEGALI
                     RRVEDPVARHCAWARLSEIDDIHLASAVTPGGVIVPAALTLAAECEADGAALARAIAA
                     GYEASVRLGRAIDGPHVLYRGVWPTCFAAPFGVAAASASLLGLDAARTAHALGIALGL
                     ASPGAGRSARWLALGHAAAGGVRAARWAQSGFTADLGLMEGALFSVYGIRADVSALTS
                     GLGDASALAQTGFKPWCAARQTMAAAQAWLELTADGFADDGTAEIRVGVPAPYAKMVD
                     HGVVPGERASYLTSVPYQLALAAVEREARFDVQQSPAKISPAVNAFMARVKVEPDAEL
                     LAQYPESWPARVDVAGRRRVVMRVPGEPQRPWTERDVLAKFERYADVDIARMALGVVE
                     GRVAPAALLARIQ"
     gene            5602..5943
                     /locus_tag="VI319_09840"
     CDS             5602..5943
                     /locus_tag="VI319_09840"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY6864190.1"
                     /translation="MQVGKSFWIAGLGLAVAGVAGCAGTYDGPYRGASNSDVRASADD
                     ATITQQIQSSLARDPNLDVANSRVSIRTENGRVALSGDVPSERDRQEAADIASRVPGV
                     TRVDNDIDVRG"
     gene            complement(5949..6896)
                     /locus_tag="VI319_09845"
     CDS             complement(5949..6896)
                     /locus_tag="VI319_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864191.1"
                     /translation="MRRWMLFLALALPALACAQTWPAKAVRLVVGFSAGGPTDLPARF
                     IADRLGTSLGQRVVVENRAGASGQIATQDVLSKPRDGYNLLLCTHFEAINAAVQRHVA
                     YKLDDIAPISQISRYYYGVVATNEIPAKDWDSFIAYAKANPGKLNYGTIGRGSAQEIL
                     ALEIGKLAGIRMTGVPYKGGAEVMNDLIAGRVQFYASPTLGVLPQYRAGKLRLLAVTS
                     AERLAAAPEIPTVSEKGLPFVRYGWLGICAGAGTPHAIVAQLNREIVAIVGSRDYRDM
                     IEKAGSVPVSSTPEELGRVLEETYEQTERVAREFDLKVD"
     gene            6936..8096
                     /locus_tag="VI319_09850"
     CDS             6936..8096
                     /locus_tag="VI319_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY6864192.1"
                     /translation="MADRIRTTHTGSLPRPPEVLAALHARFEGRGVDDAAWDALVRKH
                     VLEIVRRQVEAGIDVVGDGECSKPSFRAYLAERIGGFEPRVPKGGMPAPGPVDPNGRD
                     AAMFPDYYKGALEHSPFANTVRVAPRACVAPVRYVGQKFLRRDLANLKDAMAAAGAAE
                     GFMPSSGPIPADANEHYATEEEYFEAHGEAMREEYRAILDAGLYLQIDDPRLVSSWDG
                     RRDMDLAQYRKWMQKRIEHINHALRGLPPERIRFHTCYGVNFGPRVSDLQLEQVLDLI
                     LTIRAGFYSFEAANPRHEHEWRVAQNLKIPEGKTLIPGAVTHSSATVEHPEVVADRME
                     RWMRAAGRDRVMFGNDCGFCSTAGNTEIPETVAWAKLRALAEGARLASRRPG"
     gene            complement(8097..8462)
                     /locus_tag="VI319_09855"
     CDS             complement(8097..8462)
                     /locus_tag="VI319_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864193.1"
                     /translation="MAIKKILIVDDSPTDRQYLLEVLSKQGYECVTAQSGEEAVDKSK
                     SERPDLILMDIIMPGTDGYKATRIIKHDDATKHIPVIMCTSKNLETDRVWGMRQGAAD
                     YLVKPINAKELLAKIAAIR"
     gene            8602..10029
                     /locus_tag="VI319_09860"
     CDS             8602..10029
                     /locus_tag="VI319_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY6864194.1"
                     /translation="MKANVLRAAVLAALLPAFVAGCSASSASPIASTATTTSVTERTA
                     AGRAGLPDFTRLAEQAGPAVVNITVSKPAPAQAQGFDGDDPFSQFFRQFGVPAPKPGP
                     LRGLGSGFIVSSDGYILTNAHVVDGAKEVNVKLTDRREFRAKVIGEDTKSDIALIKID
                     AASLPTVRIGDSSHIRVGQWVVAMGSPFGFENSVTAGIVSAKSRSLSDSYVPFIQTDV
                     AVNPGNSGGPLFDLDGNVIGINSQIYSQTGGYQGLSFAIPIDIAMHVKDQLQKSGKVT
                     RGRIGVTVQGVNQSLAQSFGLPRPEGALVASVESGSPAERAGIKSGDVILAFNGQAVD
                     DSSTLPMLVADMKPGQSAPVRVWRNGAERTLQVTVGTMAAEQVAASDQSPEANSGRLG
                     LAVRALTPEEKRELKTGSGLVVEQVSGAAEHAGIQPGDVILALNDQPVRDVAQLRRLI
                     GRAGKHVALLVQRDESKMYVPVDLG"
     gene            10096..10758
                     /locus_tag="VI319_09865"
     CDS             10096..10758
                     /locus_tag="VI319_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864195.1"
                     /translation="MRLLLVEDDAMIGESIRAGLRQDGHAVDWVTDGRAAEIAIRTEP
                     YAMVLLDLGLPRKDGYAVLEDLRRGGNPVPVLILTARDAVADRVRGLDRGADDYLVKP
                     FDLDELAARIRAVLRRHAGRAEPVLAFGGLALDPATHKVAYRGRDVPVSAREFALLQA
                     LLERPGAALSRAQLEERLYGWGEEVASNSIEVHIHNLRRKLGEGAIRTVRGVGYALAS
                     DD"
     gene            10772..>11666
                     /locus_tag="VI319_09870"
     CDS             10772..>11666
                     /locus_tag="VI319_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY6864196.1"
                     /translation="MISIRRRLLAALLSALVLVGLAASAATWFAARRQANEMFDYQLE
                     QMAESLRDQAPQAEADLFAGFEHDFVVQMWGPGGVLVYLSNHNVSLPRGGAGFDTVGV
                     DGEDWRVYTMAHGGRVIQVAAPTSLRNERAAAVALRILVPIVASIPLFALLIWWLVGR
                     ELRPLGAIARAIAMRAPSSLESLPETGLPDEVRPMVSQLNGLLGRLGAAIETQKRFTE
                     DAAHELRSPLTALQLQIQLLERAASDDARRDALDQLKAGARRASRLVEQLLTMARLEP
                     ETAQEPREPVDLDRLAASVAAD"
BASE COUNT         1607 a         4166 c         4218 g         1675 t
ORIGIN      
        1 gtcgacctcg tgcccgcgtt ccatctcgag ggcctgcgcg agatcgtgta tctgcccgag
       61 gccgcgcccg acccggtgcc gtcgctcccg tccagccgct tcgcctgctc gcgcccgcgc
      121 gccgagttcc tgcaggagga gcggcgggtc tacgtctacg acttcgactc gccggacctg
      181 ttcttccaca tgctgctgca cgagatcggg cacttcgtct tcttcctcgt catcggcagc
      241 cgcgtgaaga agcactgggt gatgcatctc tatccggggt cgtcctgcat cacgccctac
      301 gcggagacga gcgcgatcga ggacttcgcc gagacctacg cgtattacgt tctcgatccc
      361 gacgcgctcg cccgggccca cgcggtgaag cacgcgttca tgcgcgactt cgtgttctcc
      421 ggcgacccgg acacgctgaa ggaacgcgcg cgcggcgcat agggggcggg cgtgttcgtg
      481 gcgatcgaca tcggcggcac tttcaccgac ctcatggcct tcgacgaggc cagcggagcc
      541 ttctcgcagg cgaagtcgct cacgacgccg gcgcagctcg tccagggcat catcgactgc
      601 ctgaagaaga gcgcggtcga tcccggcgcg atcggcgagc tgatccacgg ctcgaccacc
      661 gcgatcaaca ccctgatcga gcgcaagggc gcgaagaccg ggctcatcgt cacgcgcggc
      721 acgcgcgacg tctacgcgat cggccgcggc aaccgcccgg aggcctacga cctgttcttc
      781 catcgccacc gcccgctcgt gccgcggcat ctcacgcgcg aggtcggcgg gcggctgctc
      841 gcctcgggcg cggagcacga gccgctcgac cgcgccggcg tcgaggacgc gtgccggcgc
      901 ctcgcggccg agggcgtcga ggcggtcgcg gtctgcttcc tgcactcgta cgcgaacccg
      961 gagcacgagc gcgaggcggg cgcgatcgtg cggcggctgc tgcccggcgc gtacgtgtcg
     1021 ctctcgcacg agatcctgcg cgagtaccgc gagtacgagc gcacctcgac cacggtcgtc
     1081 aacgcctacg tcgggccgaa ggtcggcggc tacgtgcaga gcctcgcgcg cggcctgggc
     1141 ggcatcggct tccgcggcga tctcaccatc atgcagtcga acggcggcgt catgacgccc
     1201 gaggtcgcga ccgggcgccc ggtggcgatg atggagtcgg gcccggtggg cggcatcatc
     1261 gccgcggcgc gcgtcgcggc cgcgctcggc cacgccgacg cgatctcctt cgacatgggc
     1321 ggcacgaccg cgaaggcgag cctgatccgc gagggccagc cggcgaccgc gagcggctac
     1381 cacgtcggcg gctacgcgag cggccacccg gtgatggtgc ccgtcatcga cgtgatcgag
     1441 gtcggcgcgg gcggcggcag catcgcctgg atcgacgacg tcggcgcgct caaggtgggc
     1501 cctcgcagcg cgggcgccga tcccgggccg gtttgctacg gcgcgggcgg gaccgagccg
     1561 accatcaccg acgcgaacct ggttctcggc cgcctggatc ccgacgactt cctcggcggg
     1621 cagatggtgc tcgacgcgaa aggcgcggag cgcgcgctcc aggagaagat cgcgcggccg
     1681 ctcgggctct cgagcgtcgc cgcggcgcag gcgatcgtcg acatcgccgt gtcccggatg
     1741 tcgctcgcgg tgcgcgaggt gtcggtcgcg aagggccacg atccgcgcga cttcgcgctc
     1801 gtcgcctcgg gcggcgcggg gccgctgcac gtggtcgcgg tggcgcgcga gctcgccatc
     1861 ccgaccgtga tcgtgccgcg ctttcccgcg catttctcgg cgctcggcat gctcgccgcc
     1921 gacgagcggc acgacgtggc gcgcaccttc tacgccgacc tcgcacgcgt cgacttcgca
     1981 gcgctgcgcg ccgcgctcga cgagatgcgc cgcgaaggtc tcgcggggct gcgcaaccgc
     2041 cgcagcatcg aggagcggct gcagctcgac ctgcgctacg tgggccagga gttcacgctg
     2101 ccggtgccgg cgaccgaggc gcagctcgcg gacgccgacc gcgccgccat ccgcgccgcg
     2161 ttcgacgcgc tgtacgagca gcgctacgcg cacagctcgc cgcgcgagcc ggtcgagatc
     2221 gtcaacgtgc ggctcgcggt gatcggcaag cggccgcgga tggagtttcc gaagctgcgc
     2281 gaaggcacga aggcggagcc ggcctcggtg caggaggtgt tcttcccggg ctcggagcgt
     2341 cccgtgcgct gcgctgtcta cgagcgcgag tcgctgcccg cgggggcgcg cttcgccggc
     2401 ccggcgctgg tgcgcgagca cggcacgacc acggtgatgc cgccggccac ggaatgccgg
     2461 gtggccgcga ccggcgagct ggtcatcgcc gagatgggat cggcccgatg aaggacctgg
     2521 acccggtcac gctcgaggtg gtgcgcaacg cgctgcccgc gatcgcgaac gagatggcgg
     2581 ccgacctcca gcgcacctcg tacaacatga tgatctacga ggtgcgcgac ttctgcaccg
     2641 cgctgctcga cgtcgacggc gcgctcatct cgcagaacgt cggcggcgtg tcgcacttcg
     2701 tcgccgatct cggcgtggtg gtcgccgacg gcgtgatgcg ctacgggcgc gagggcttcg
     2761 cgccgggcga cgtgatcatc acgaatcacc aggcggtcgc cggccagcac ctgaacaacg
     2821 tcgtgatcta cgtgccgtgc ttcttcgagg gcgagctgct cgcgttcgcg atggtgcgcg
     2881 cgcactggat cgacgtcggc ggcaggagca cgggcttcgg cgcggggccc gacgttcccg
     2941 atccgtggca ggagggcctg cagctcgacc agctgaagat ccacgaagcg ggcaagctcg
     3001 acgagacgct gctgcgcgtg atccgcgaca acatccgctt cccggaatcg tcgctcggcg
     3061 acatgaactc gcagatcgcg gcgtgccgcc tgggcgcgcg ccgtctcgac gagctgttcc
     3121 gccgctacgg gcgcgcgacg ctgctcgccg cgatccggcg catcttcgag gagaccgagc
     3181 gcaagtgccg caacgtggtc gcgcagctcg ccgacggcga gtacgaggcc gagtccttct
     3241 tcgacgacga cggggtgacg aagggcgagc gcgtgcgcat ccacgccaag gtcaccgtcg
     3301 acaaaggcgc gatgacgatc gacctctcgg gctgctcgcc cgagcgcaag gccgcggtca
     3361 actcgcgcac gctcgccgcg gcgcgagtcg cctacaaggc gctgaccggg ccgctcgagc
     3421 cggtgaacga gggctcgttc cgcgcgctcg aggtgatcat ccccgagggc agcatcatga
     3481 tggcgcgcta cccggcgccg atggcggggt ggagcctgtt cgtgccgatg gtggtcgaga
     3541 ccatcgtcac ctcgctcgcg cgcgcgatgc ccgagcgcat cccggccgcg caccacgcgc
     3601 tgctcggcgg cgcggtggtg ttcttcggga ccaatccgga gaccgggcgg cgcttcgtcg
     3661 tgcagagcgt cgagggcggc ggctgggggg ggcggccgtt cgaggacggc gagtcggcga
     3721 cggtctccgt gtgccagggc gacgtgcgga acggctcgat cgagggcatc gagatgaagt
     3781 gcccggtgct gatcgaggag cgcgcgctgc gtcccgactc gggcggcgcg gggaagtggc
     3841 gcggcgggct cggtgtgagc ctgcgcgtgc acaacttcgt cgagggccgc tggaacttcg
     3901 agcgcgcgag ccgcgccggc tgcccgccgt ggggattgtg gggcgggcgc gcgggcgcga
     3961 gcggcggcta ctggctgagc aagcccggcg acgcggagcc gcgcggcatg ttcggcagcc
     4021 agcacgccgt gccccgggac tgcaccgtga tcgtgcgcac gggcggcggc ggcggctggg
     4081 gcgatccgct cgagcgcgat ccgaagcgcg tgcgcgagga cgcgatcgag gagctggtgt
     4141 cgatcgacgc ggcgcgcgag cactacggcg tggtgctggg gcccggcttc gagatcgacg
     4201 tggaagcgac cgccgcgctg cgcgcgaagc aatcccggaa gaagcagcgc cagccggcgt
     4261 gaccgcgctc gagcgattcg gcgagtggat cgccggggcc gccatcccga ccgaaggcgt
     4321 cgcgctgcac ctcgtcgacg cggtcgcggc ctggatcgcg ggcgccggca cggccgaggg
     4381 cgcgctgatt cggcgcgtgg aggacccggt cgcgcggcat tgcgcgtggg cgcgcctgtc
     4441 cgagatcgac gacatccatc tcgcctcggc ggtgacgccg ggcggcgtga tcgttccggc
     4501 cgcgctgacg ctcgcggcgg aatgcgaagc cgacggcgcc gcgctcgcgc gcgcgatcgc
     4561 cgccggctac gaggccagcg tgcgcctcgg gcgcgccatc gacggtccgc acgttctcta
     4621 ccgcggcgtg tggccgacgt gcttcgcggc gccgttcggc gtcgccgcgg cgagcgcgag
     4681 cctgctcggc ctcgacgccg cgcgcaccgc gcacgcgctc ggcatcgcgc tcggtctcgc
     4741 ctcgcccggc gctggccgca gcgcgcgctg gctcgcgctc ggccacgccg ccgcgggcgg
     4801 cgtgcgcgcc gcgcgctggg cgcagtccgg gttcacggcg gacctcgggc tgatggaagg
     4861 cgcgctcttc tccgtatacg gcattcgcgc cgacgtctcc gcgctgacct ccggactggg
     4921 cgacgcgagc gcgctcgcgc agaccggctt caagccctgg tgcgccgcgc gccagaccat
     4981 ggccgcggcg caggcgtggc tcgagctcac cgccgatggc ttcgcggacg acggcaccgc
     5041 cgagatccgc gtcggcgtcc ccgcgcctta cgcgaagatg gtcgaccacg gcgtcgtgcc
     5101 cggcgagcgc gcgtcgtacc tcaccagcgt cccgtaccag ctcgcgctcg cggcggtcga
     5161 gcgcgaagcg cgcttcgacg tccagcagag tcccgccaag atctcgccgg cggtgaacgc
     5221 cttcatggcg cgcgtgaaag tcgagcccga cgccgagctg ctcgcccagt atcccgagtc
     5281 gtggcccgcg cgcgtcgacg tcgccgggcg ccggcgcgtc gtcatgcgcg tccccggcga
     5341 gccgcagcgg ccgtggaccg agcgcgacgt gctggcgaaa ttcgagcgct acgccgacgt
     5401 cgacatcgcg cgcatggcgc tcggggtcgt ggaaggacgc gtggcgcctg ccgcgctgct
     5461 cgcgcgaatc caataggcag cgccggagcg tcgtcgtttc catcttgttg atggacagat
     5521 tccggcggaa ccgtcccgcc tccgcctcgt cgtactgatg ttgcggcccc gccggggccg
     5581 cgcttcgtat acggaggctt catgcaagtc ggcaaatctt tctggatcgc gggtctcggg
     5641 ttggcggtgg ccggggtcgc ggggtgcgcg ggcacctacg acgggccgta tcgcggcgcg
     5701 tccaacagcg acgtgcgcgc gagcgcggac gacgcgacga tcacgcagca gatccaatcg
     5761 agcctcgcgc gggacccgaa tctcgacgtg gcgaactcgc gcgtgagcat caggaccgag
     5821 aacgggcgcg tggcgctgag cggcgacgtg ccgagcgagc gcgaccgcca ggaagccgcc
     5881 gatatcgcgt cgcgcgtgcc gggcgtgacg cgggtcgaca acgacatcga cgtgcgcggg
     5941 tagccgcgtc agtcgacctt caggtcgaac tccctcgcga cgcgctcggt ctgctcgtag
     6001 gtctcctcga gcacgcgccc gagctcctcg ggcgtcgacg acaccggcac cgagcccgcc
     6061 ttctcgatca tgtcgcggta atcgcgcgag ccgacgatcg cgacgatctc gcggttgagc
     6121 tgcgcgacga tcgcgtgcgg cgtgccggcg cccgcgcaga tgccgagcca gccgtagcgc
     6181 acgaacggca gtcccttctc ggagacggtc gggatctcgg gcgcggcggc gaggcgctcg
     6241 gcgctcgtca ccgcgagcag tcgcagcttg cccgcgcggt actgcggcag cacgccgagc
     6301 gtgggcgagg cgtagaactg cacgcgcccc gcgatcaggt cgttcatcac ctcggcgccg
     6361 cccttgtaag gcacgccggt catgcggatg ccggcgagct tgccgatctc gagcgcgagg
     6421 atctcctgcg ccgagccgcg cccgatcgtg ccgtagttga gcttgccggg gttcgccttg
     6481 gcgtaggcga tgaagctgtc ccagtccttg gccgggatct cgttcgtggc cacgacgccg
     6541 tagtagtagc gcgagatctg gctgatcggg gcgatgtcgt cgagcttgta cgcgacatgg
     6601 cgctgcaccg cggcgttgat cgcctcgaag tgcgtgcaca gcagcaggtt gtagccgtcg
     6661 cgcggcttcg acagcacgtc ctgcgtcgcg atctggccgc tcgcgccggc gcggttctcg
     6721 accaccacgc gctggccgag cgacgtgccc agccggtcgg cgatgaagcg cgcgggcagg
     6781 tcggtcgggc cgccggcgct gaagccgacg acgaggcgca cggccttcgc gggccaggtt
     6841 tgcgcgcagg cgagcgcggg aagcgcgaga gcgaggaaga gcatccagcg gcgcacggtc
     6901 acctccgtcg tgagcaaccg ttaatataag gcgatatggc cgatcgcatc cgcaccaccc
     6961 acaccggcag ccttccgcgc ccgcccgagg tgctcgccgc gctgcacgcg cgcttcgagg
     7021 gccgcggcgt cgacgacgcg gcctgggacg cgctcgtgcg caagcacgtg ctcgagatcg
     7081 tgcgcaggca ggtcgaggcc ggcatcgacg tcgtcggcga cggcgagtgc tcgaagccga
     7141 gcttccgcgc ctacctcgcc gagcgcatcg gcggcttcga gccgcgcgtt ccgaaaggcg
     7201 ggatgcccgc gcccggcccg gtcgacccga acgggcgcga cgcggcgatg ttccccgact
     7261 actacaaggg ggcgctcgag cacagcccgt tcgcgaacac cgtgcgcgtc gcgccgcgcg
     7321 cctgcgtcgc acccgtccgc tacgtcggcc agaaattcct ccggcgcgac ctcgccaatc
     7381 tcaaggacgc gatggcggcc gcgggcgcgg ccgaaggctt catgccctcg tcgggcccga
     7441 tccccgccga cgcgaacgag cactacgcga ccgaggagga gtacttcgag gcgcacggcg
     7501 aggcgatgcg cgaggagtac cgcgccatcc tcgacgcggg gctctatctg cagatcgacg
     7561 acccccggct ggtcagctcc tgggacggcc gccgggacat ggacctcgcg caatatcgaa
     7621 agtggatgca gaagcgcatc gagcacatca accacgcgct tcgcgggctg ccgcccgagc
     7681 gcatccgctt ccacacctgc tacggcgtga acttcggccc gcgcgtctcc gacctgcagc
     7741 tcgagcaggt gctcgacctg atcctgacga tccgcgccgg gttctactcc ttcgaggcgg
     7801 ccaacccgcg ccacgagcac gaatggcgcg tcgcgcagaa cctgaagatc cccgagggga
     7861 agacgctgat ccccggcgcg gtcacgcact ccagcgccac ggtcgagcac cccgaggtcg
     7921 tcgccgaccg catggagcgc tggatgcgcg ccgccgggcg cgaccgcgtg atgttcggga
     7981 acgactgcgg cttctgctcg accgcgggca acaccgagat cccggagacg gtcgcctggg
     8041 cgaagctgcg cgcgctcgcc gagggcgcgc ggctcgcgtc gcggcgcccc ggctgatcag
     8101 cggatcgccg cgatcttcgc gagcagctcc ttggcgttga tcggcttcac caggtagtcc
     8161 gccgcgccct ggcgcatgcc ccagaccctg tcggtctcga ggttcttgct cgtgcacatg
     8221 atcaccggga tgtgcttggt cgcgtcgtcg tgcttgatga tgcgggtcgc cttgtagccg
     8281 tcggtgccgg gcatgatgat gtccatgagg atcaggtcgg ggcgctcgct cttggacttg
     8341 tcgactgcct cttcgccgct ctgcgcggtg acgcactcgt agccctgctt gctcagcacc
     8401 tcgagcaggt actggcggtc ggtgggcgaa tcgtcgacga tcaggatctt cttgatggcc
     8461 atggttttct cctcctgggc cccctatata tatcagttct gcggtcttcg taacaggtta
     8521 cagtccgtta cagcggcctt aagcccttct taagcttccc cacctagcat gacggcattc
     8581 gatcctgcta gggagccgag catgaaagca aacgtccttc gggctgcggt cctcgccgcc
     8641 ctgctgccgg ccttcgtcgc cggctgttcc gcctcgtcgg cgtcgccgat cgcgtccacg
     8701 gcgacgacca cctccgtcac cgagcgcacg gccgccggcc gtgccgggct tcccgacttc
     8761 acccggctcg ccgagcaggc gggccccgcg gtcgtgaaca tcaccgtgtc caagcccgcg
     8821 ccggcccagg cgcaggggtt cgacggcgac gatccgttct cgcagttctt ccgccagttc
     8881 ggcgtcccgg cgcccaagcc cggtcccttg cgcggcctcg gctcgggctt catcgtgagc
     8941 tccgacggct acatcctcac caacgcccac gtcgtcgacg gcgcgaagga ggtcaacgtc
     9001 aagctcaccg accgccgcga gttccgggcg aaggtcatcg gcgaagatac gaagagcgac
     9061 atcgcgctga tcaagatcga cgccgccagc ctgccgacgg tgcgcatcgg cgactcgtcg
     9121 cacatccggg tcggccagtg ggtggtggcg atgggctcgc ccttcggctt cgagaacagc
     9181 gtgaccgcgg gcatcgtctc ggcgaagtcg cgctcgctct ccgacagcta cgtgccgttc
     9241 atccagaccg atgtcgcggt gaacccgggt aactccggcg ggccgctgtt cgacctcgac
     9301 ggcaacgtca tcggcatcaa ttctcagatc tacagccaga cgggcggcta ccagggcctg
     9361 tcgttcgcga tcccgatcga catcgcgatg cacgtgaagg accagctgca gaagagcggc
     9421 aaggtcacgc gcggccgcat cggcgtcacg gtgcaggggg tgaaccagag cctcgcccag
     9481 tccttcggcc tgccgcggcc cgagggcgcg ctcgtcgcct cggtcgagag cggcagcccg
     9541 gcagagcgcg cgggcatcaa gtcgggcgat gtcatcctcg ccttcaacgg gcaggcggtc
     9601 gacgattcgt ccacgctgcc catgctggtc gccgacatga agcccgggca gtcggcgccc
     9661 gtccgcgtct ggcgcaacgg cgccgagcgc acgctgcagg tgacggtcgg gacgatggcg
     9721 gccgagcagg tcgccgcgag cgaccagtcc cccgaggcca actcgggccg gctcggcctc
     9781 gcggtgcgcg cgctcacgcc cgaggagaag cgcgagctga agacgggctc cggcctcgtg
     9841 gtcgagcagg tgagcggcgc cgccgagcac gcgggcatcc agcccggcga cgtcatcctc
     9901 gcgctcaacg accagccggt gcgcgacgtc gcgcagctca ggcggctcat cgggcgcgcg
     9961 ggcaagcacg tggccctgct ggtgcagcgc gacgagtcca agatgtacgt gccggtcgac
    10021 ctcggttgac cgggcccgtg ccggcggcgg cccgactgat ctcctcgggc cgccgcccgt
    10081 tttagaatcg ccgccatgcg cctgctgctg gtcgaagacg acgccatgat cggcgagagc
    10141 atccgcgcgg ggctgcgcca ggacggccac gcggtcgact gggtcaccga cggccgcgcc
    10201 gccgagatcg cgatccgcac cgagccctac gcgatggtgc tgctcgacct gggcctgccg
    10261 cgcaaggacg gctacgcagt gctcgaggac ctgcgccgcg gcggcaaccc cgtgccggtg
    10321 ctcatcctca ccgcgcgcga cgcggtcgcc gatcgcgtgc gcggcctcga ccgcggcgcc
    10381 gacgactacc tggtgaagcc cttcgacctc gacgagctcg ccgcgcgcat ccgcgcggtg
    10441 ctgcgccggc acgcggggcg cgccgagccg gtgctcgcct tcggcggcct cgcgctcgac
    10501 ccggcgacgc acaaggtggc gtaccggggt cgcgacgtgc ccgtgtcggc gcgcgagttc
    10561 gcgctcctgc aggcgctgct cgagcgcccg ggcgcggcgc tgtcgcgcgc gcagctcgag
    10621 gagcgcctct acggctgggg cgaggaggtc gcgagcaact cgatcgaggt gcacatccac
    10681 aacctgcgca ggaagctcgg cgagggcgcg atccgcacgg tgcgcggcgt gggctacgcg
    10741 ctcgcgtcgg acgactagcg gcgcccgcgc ggtgatctcg atccgcagga ggctgctcgc
    10801 cgcgctgctc tcggcgctgg tcctcgtcgg cctcgcggcg agcgcggcga cctggttcgc
    10861 ggcgcggcgg caggccaacg agatgttcga ctaccagctc gagcagatgg ccgagtcgct
    10921 gcgcgaccag gcgccgcagg ccgaggccga cctgttcgcg ggcttcgagc acgacttcgt
    10981 cgtgcagatg tgggggccgg gcggcgtgct ggtctacctc tccaaccata acgtcagcct
    11041 gccgcgcggc ggcgcgggct tcgacaccgt cggcgtcgac ggcgaggact ggcgggtgta
    11101 cacgatggcg cacggcggcc gcgtgatcca ggtcgcggcg ccgaccagcc tgcgcaacga
    11161 gcgcgcggcc gcggtcgcgc tgcgcatcct ggtgccgatc gtcgcgtcga tcccgctgtt
    11221 cgcgctgctg atctggtggc tggtcgggcg cgagctgcgc ccgctcggcg ccatcgcgcg
    11281 cgcgatcgcg atgcgcgcgc cctcgtcgct cgagtcgctg cccgagaccg ggctgcccga
    11341 cgaggtgcgg cccatggtgt cgcagctgaa cggcctgctc gggcggctcg gcgcggcgat
    11401 cgagacgcag aagcggttca ccgaggacgc ggcgcacgag ctgcgctcgc cgctcacggc
    11461 gctgcagctg cagatccagc tgctcgagcg cgccgcgagc gacgacgcgc ggcgcgacgc
    11521 gctcgaccag ctcaaggccg gcgcgcgccg cgcctcgcgc ctggtcgagc agctgctgac
    11581 catggcgcgc ctcgagcccg agacggccca ggagccgcgc gagccggtcg atctcgaccg
    11641 gctcgccgcg agcgtcgccg ccgact
//
LOCUS       DATDMW010000231         4540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_72384, whole genome shotgun sequence.
ACCESSION   DATDMW010000231 DATDMW010000000
VERSION     DATDMW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72384"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3438
                     /locus_tag="VI319_09875"
     CDS             <1..3438
                     /locus_tag="VI319_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6864197.1"
                     /translation="LIADAETTSEAIRSVIRAIFQSEGWECGRYLRPQADAALRQVED
                     WGIDEPQVQAFLERARSFDYTPGKGLAGGVLASGQPLWVADSTGDARALLRRHFGDVG
                     LRGAFVFPVKSAGRTIGVLAFNSRERREPDERLLSAILAIGSQIGQFIERKRVEEELR
                     RFRAAMDGSADIIVLVDPTAARFVDANDTACRMLGYTREELLRVPPEEAVGVPREKLA
                     AEYAEMIEGRASAGGMLGHYRRKDGSSFPFESRRQVVRSGEGVLIAAISRDISKRVAA
                     ERALRESEARFRTVIGSANEGILVFDREMRIVSANGAAARIIGVPEEKLVGAPGFISL
                     VPCVLEDGTPVTQENRAPVLAFRARRGVAGRVVGIRRASGDITWLQTNTALLYDEGRS
                     AEKGDEPYGAVATLADITALKRDEALLRLEQRVARAFDTEPDAPAALRAAIQAICESE
                     QWDCGRYLEVAASAKGAPGPELRAFAHWSSDDPAVLRYIEHVRPMAFKPGEGLVGMVW
                     ETRQPMWVRDIEHGPVGGRPGLALGMGARSALVVPVLALDAVIGVFIFHSRRVREADE
                     GLLQAMQLIGAQIGQLVRRAKAEAAVRESEARFRSLCELSSDVFWEQDAQFRFTAITD
                     SSGRLDPAAILGRARWEIGYAGVTAQEWAAHRAILEAHQPFRDLELCRVDAAGRETWY
                     NESGAPVFDAAGAFAGYRGVGRDITARKLDERRIRHLANHDALTGLPNRSAFSELLNA
                     AREAARRRSRSLAVMFVDLDRFKVINDTLGHEAGDEVLCEVARRLRHTLRASDVVARL
                     GGDEFVVMIPELESSLQAETAARKVLGSMLPPMMAAGRELSVTASIGICLYPQDGEDE
                     QALMKNADAAMYRAKEAGKNNFKFHHPATDRRSLERLAMETSLRRALERNEFFLHYQP
                     RISLATGAVTGVEALARWRHPELGVVPPAEFIALAEETGAIHDIGRWVLEAACAQAMQ
                     WQRDGLAPVHVAVNISARQFGADNLVAQVEAALAASGLPPARLELEVTESVVAQSIER
                     AAQILGSIRALGVRVALDDFGTGYSSLAQLKRFPIDTIKIDRAFVSELPHNLDDAAIA
                     RAIIAMGKSLRLVVVAEGVETPQQHAFLQAHGCDEMQGYLISRPLSPADCASFLRRG"
     gene            complement(3378..>4540)
                     /locus_tag="VI319_09880"
     CDS             complement(3378..>4540)
                     /locus_tag="VI319_09880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY6864198.1"
                     /translation="RCLVGTLSHLDEVFPSHRVAKGDAARPLARLDAPPPLRYRHDGR
                     QDDLDGFLAHNRNTGLLVLEGDTIVAERYQYERRPEHRFQSYSMAKTVVGMLVGLALA
                     DGKIASIDDRAGQYVPELKGHPYGETTLRHLLTMSSGVKFSETYDGKDDVAILARNTI
                     FGQGPGGPAALLPFRERVRPAGTIFSYASAESEVLGLVVRAAVGKPLAEYLSQKIWKP
                     MGAEADATWIVDGGGYELGYMGINATLRDWGRLGLLLARYGELDGRQIIPAEWIKAAT
                     RPDAPHLRVGYATRFNGYGYQTWLVDERGRFALLGVRGQAVFVDPQTRLVVVHTAVHA
                     SSRDPARGEQFALFYGLLESLEKPGPRLRGDDDYPRRRNEAQSAGESGRLIR"
BASE COUNT          622 a         1657 c         1665 g          596 t
ORIGIN      
        1 ctcatcgcgg atgccgagac gacgagcgag gcgatccggt cggtgatccg cgccatcttc
       61 cagtcggagg gctgggagtg cggccgctac ctccgcccgc aggccgacgc ggcgctgcgc
      121 caggtcgagg actggggcat cgacgagccg caggtgcagg cgttcctcga gcgcgcgagg
      181 agcttcgact acacgcccgg caagggcctc gccggcggcg tgctcgcctc cggccagccg
      241 ctctgggtcg ccgactcgac gggcgacgcg cgcgcgctgc tgcgccggca cttcggcgac
      301 gtcggcctgc gcggcgcgtt cgtcttcccg gtgaagagcg ccggccgcac gatcggcgtg
      361 ctcgccttca acagccgcga gcggcgcgag cccgacgagc gcctgctctc cgcgatcctc
      421 gccatcggca gccagatcgg ccagttcatc gagcgcaagc gcgtcgagga ggagctgcgc
      481 cgcttccgcg cggcgatgga cggctcggcc gacatcatcg tgctcgtcga tcccacggcg
      541 gcccgcttcg tggacgcgaa cgacacggcg tgccgcatgc tcggctacac gcgcgaggag
      601 ctgctgcgcg tgccgcccga ggaggcggtc ggcgtgccgc gcgagaagct cgccgccgag
      661 tacgccgaga tgatcgaggg ccgcgcgagc gcggggggca tgctcggcca ctaccggcgc
      721 aaggacggca gcagcttccc cttcgagtcg cgccgccagg tggtgcgctc cggcgaaggc
      781 gtgctgatcg ccgccatctc gcgcgacatc agcaagcgcg tcgccgccga gcgcgcgctg
      841 cgcgagagcg aggcgcgctt ccgcaccgtg atcggctcgg cgaacgaggg catcctcgtc
      901 ttcgaccgcg agatgcgcat cgtctcggcg aacggcgccg ccgcgcgcat catcggcgtg
      961 ccggaggaga agctggtcgg cgcgcccggg ttcatctcgc tcgtgccctg cgtgctcgag
     1021 gacggcacgc cggtgacgca ggagaaccgc gcgccggtgc tcgccttccg cgcgcggcgc
     1081 ggcgtggcgg gccgcgtggt cggcatccgc cgcgcctcgg gcgacatcac ctggctgcag
     1141 accaacaccg cgctcctcta cgacgagggc cgctcggccg agaaaggcga cgagccctac
     1201 ggcgcggtcg cgacgctcgc cgacataacc gcgctaaagc gcgacgaggc gctgctgcgc
     1261 ctcgagcagc gcgtcgcgcg cgccttcgac accgagccgg acgcgcccgc cgcgctgcgc
     1321 gccgcgatcc aggcgatctg cgagagcgag cagtgggact gcggccgcta cctcgaggtg
     1381 gcggcgagcg cgaagggcgc gccgggcccc gagctccgcg ccttcgccca ctggtcgagc
     1441 gacgacccgg ccgtgctgcg ctacatcgag cacgtgcggc cgatggcgtt caagccgggc
     1501 gagggcctgg tcggcatggt ctgggagacg cgccagccga tgtgggtgcg cgacatcgag
     1561 cacggcccgg tgggcgggcg gcccggcctc gccctcggca tgggcgcgcg cagcgcgctc
     1621 gtcgtgcccg tgctcgcgct cgacgcggtg atcggcgtgt tcatcttcca cagccggcgc
     1681 gtgcgcgagg ccgacgaggg cctgctgcag gcgatgcagc tcatcggcgc gcagatcggc
     1741 cagctcgtgc gccgcgccaa ggccgaggcc gcggtgcgcg agagcgaggc gcgcttccgc
     1801 agcctgtgcg agctctcctc cgacgtgttc tgggagcagg acgcgcagtt ccgcttcacc
     1861 gccatcaccg attcctccgg gcgcctcgac ccggcggcca tcctcggccg cgcgcgctgg
     1921 gagatcggct acgcgggcgt gaccgcgcag gagtgggccg cgcaccgcgc gatcctcgag
     1981 gcgcaccagc ccttccgcga cctcgagctc tgccgcgtcg acgcggccgg ccgcgagacc
     2041 tggtacaacg agagcggcgc gccggtgttc gacgcggcgg gcgccttcgc cggctaccgc
     2101 ggcgtcggcc gcgacatcac cgcgcgcaag ctcgacgagc ggcgcatccg ccacctcgcc
     2161 aaccacgacg cgctgacggg cctgcccaac cggagcgcct tcagcgagct gctgaacgcc
     2221 gcccgcgagg cggcgcgccg gcgctcccgc tcgctcgcgg tgatgttcgt cgacctcgac
     2281 cgcttcaagg tgatcaacga cacgctcggc cacgaggcgg gcgacgaggt gctgtgcgag
     2341 gtggcgcggc gcctgcgcca cacgctgcgc gcgagcgacg tggtggcgcg cctgggcggc
     2401 gacgagttcg tggtgatgat ccccgagctc gagtcgtcgc tgcaggccga gaccgcggcg
     2461 cgcaaggtgc tcggctccat gctgccgccg atgatggccg ccggccgcga gctctcggtc
     2521 accgcgagca tcggcatctg cctctacccg caggacggcg aggacgagca ggcgctgatg
     2581 aagaacgccg acgcggcgat gtaccgcgcc aaggaggcgg gcaagaacaa cttcaagttc
     2641 caccatccgg cgaccgaccg gcggtccctg gagcgcctcg ccatggagac gagcctgcgg
     2701 cgcgcgctcg agaggaacga gttcttcctg cactaccagc cgcgcatctc gctcgccacc
     2761 ggcgccgtga ccggcgtgga ggcgctcgcc cgctggcgcc atcccgagct cggcgtggtg
     2821 ccgcccgccg agttcatcgc gctggccgag gagaccggcg cgatccacga catcggccgc
     2881 tgggtgctcg aggcggcgtg cgcgcaggcg atgcagtggc agcgcgacgg cctcgcgccg
     2941 gtgcacgtcg cggtgaacat ctcggcgcgg cagttcggcg ccgacaacct cgtcgcgcag
     3001 gtcgaggcgg cgctcgccgc gagcggcctg ccgccggcgc ggctcgagct cgaggtcacc
     3061 gagagcgtgg tggcgcagag catcgagcgc gcggcgcaga tcctcggcag catccgcgcg
     3121 ctcggcgtgc gcgtcgcgct cgacgacttc ggcaccggct actcgtcgct cgcgcagctc
     3181 aagcgcttcc cgatcgacac catcaagatc gaccgcgcgt tcgtctccga gctgccgcac
     3241 aacctcgacg acgcggcgat cgcgcgggcg atcatcgcca tgggcaagag cctgcgcctg
     3301 gtggtcgtcg ccgaaggcgt cgagaccccg cagcagcacg ccttcctgca ggcgcacggc
     3361 tgcgacgaga tgcagggcta cctgatcagc cggccgctct cgcccgcgga ttgcgcctcg
     3421 ttcctgcgcc ggggatagtc gtcgtccccg cgaaggcggg gacccggttt ctcgagcgat
     3481 tccagcaggc cgtagaagag cgcgaactgc tcgccgcgcg ccggatcgcg cgacgacgcg
     3541 tgcaccgcgg tgtgcaccac gacgaggcgc gtctgcgggt cgacgaagac cgcctggccg
     3601 cgcacgccga ggagcgcgaa gcgcccgcgc tcgtccacga gccaggtctg gtagccgtag
     3661 ccgttgaagc gcgtcgcgta gcccacgcgc aggtgcggcg catcgggccg cgtcgccgcc
     3721 tttatccact cggcgggaat gatctgccgg ccgtcgagct cgccgtagcg agcgagcagc
     3781 aggccgagcc ggccccagtc gcgcagcgtc gcgttgatcc ccatgtagcc gagctcgtag
     3841 ccgccgccgt cgacgatcca cgtcgcgtcg gcctcggcgc ccatgggctt ccagatcttc
     3901 tgcgagaggt attcggcgag cggcttgccg accgcggcgc gcaccaccag gccgagcacc
     3961 tcgctctccg cggacgcgta ggagaagatc gtcccggccg gacgcacgcg ctcgcggaag
     4021 ggcagcagcg ccgccggccc gccggggccc tgcccgaaga tcgtgttccg cgcgaggatc
     4081 gccacgtcgt ccttgccgtc gtaggtctcg ctgaacttca cgcccgagga catggtgagc
     4141 aggtggcgca gcgtggtctc gccgtacgga tggcccttca gctcgggcac gtactgcccg
     4201 gcgcggtcgt cgatcgaggc gatcttcccg tcggcaaggg cgaggccgac cagcataccg
     4261 acgaccgtct tcgccatcga gtaggactgg aagcggtgct cgggcctgcg ctcgtactgg
     4321 tagcgctcgg cgacgatcgt gtcgccctcg aggacgagca gcccggtgtt gcggttgtgc
     4381 gcgaggaagc cgtcgaggtc gtcctggcgc ccgtcgtgcc ggtagcgaag cggcggcggc
     4441 gcgtcgaggc gcgcgagcgg gcgcgcggca tcgcccttcg cgacccggtg cgacgggaac
     4501 acctcgtcca ggtgcgagag cgtgccgacg aggcagcgct
//
LOCUS       DATDMW010000232         4483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_319760, whole genome shotgun sequence.
ACCESSION   DATDMW010000232 DATDMW010000000
VERSION     DATDMW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319760"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VI319_09885"
     CDS             complement(<1..378)
                     /locus_tag="VI319_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY6864199.1"
                     /translation="MVHPRIRKTAASVAVAGALAVGYSQAHASAFALQEQNASGLGNA
                     FAGQAATAENASTIYFNPAGMSFLPAGPQLSLGGDYIKPSTKFSDGGSTAAGFPGVAA
                     TQRPLGGNGGEAGNPAFVPNIYFA"
     gene            complement(501..1700)
                     /gene="tyrS"
                     /locus_tag="VI319_09890"
     CDS             complement(501..1700)
                     /gene="tyrS"
                     /locus_tag="VI319_09890"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY6864200.1"
                     /translation="MSDADRQLGILKRGASELIVEEELRDKLKAGRPLRVKLGMDPTA
                     PDLHLGHTVVINKLRQFQELGHHVQFLIGDFTGMIGDPSGKNVTRPPLSREQILRNAA
                     TYQDQVFKILDAKRTEVLFNSKWCDELGAAGMVKLAATYTVARMLERDDFAKRFHDSK
                     PIAVHEFLYPLMQGYDSVAMKSDVELGGTDQKFNLLVGRELQKHHGQPQQSILMMPLL
                     EGTDGKDKMSKSLGNYVGISEPAETIFGKLMSISDDLMWRYIELLSFEPAETVERWKR
                     EVESGLNPRDVKVRFAREIVARFHSEREAERAQADFEARFREGALPEDMPEVRVAAGA
                     KPMAIAQVLKAAGLTASTSEALRMIEQGGIRIDGEKVSDKGTQIPAGKTVVLQVGKRK
                     FARVTLG"
     gene            1762..3063
                     /locus_tag="VI319_09895"
     CDS             1762..3063
                     /locus_tag="VI319_09895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY6864201.1"
                     /translation="MNEGKTRIVTQRYLQAARRPWLAAAFAVPVFGVVAAFATVQPNP
                     EPVLTRAVVEALPLSAEIAVQGSATPYFHEERFQRGDTVTAVLARLGADGDDARALLA
                     SPGARRLVRLMRPGTTVQATTQDAKLQSMWFLSGTDSLTIVERKDGAFTASERTVSLS
                     REVELRTGQIASSLFAATDAAGVPDNVASQIADIFAGDVDFQRDLRRGDRFTVAFEMF
                     YHEGRPIRSGRLLAADFTNQRHTYRAIWFQPADGPGGYYSPDGKNLRKAFLRSPLEYS
                     RISSGFGMRNHPILQQWRAHKGVDYAAPIGTRVRATGDGVVDFAGKRNGYGNVIVLRH
                     NGGYSTYYAHLNGFARGVQRGVRVAQGDIIGYVGQSGWATGPHLHYEFHVRNEYRNPL
                     TLAMPASNPVPHHLLIAFFQASQPLAAQLDLLKNTDVALLE"
     gene            3123..>4483
                     /locus_tag="VI319_09900"
     CDS             3123..>4483
                     /locus_tag="VI319_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6864202.1"
                     /translation="MGEKKRRQAAGRPSAHDARSTISRALELMRSGDGEAALALCEPL
                     FDEPLSDPEALHYLGYLAIELGRAGRAVGVLQRAIRQQPADAHLHNTLAIAFQRLGQP
                     DSAVETLERAIALAPGVHELYNNLGNSLSALGRHAEAAASYLQASRIAPEIGAYRSAF
                     AEALSHVRGVPDPSLRPDLLQALAHEQVDPGTLTDTVNTLLRTDPGFVELERLAAAGG
                     LSAASLAAPPPFLRDELLLRALGETILGDPGIERLLTGLRRVALECEVRGEPCPAVPT
                     ELVCGLALQCFATEYAWWVTPEEEQLLPALRAKLFPAPADASPSWERAVALLACYVAL
                     HELPEAGELQPRGGPLEPVLRRQVFEPREEARLRPLIPGLDPIDDAVSHAVRNQYETH
                     PYPRWTRLGRFDHAAPLGVVLRELFPRKKLAAPDGPLQILVAGCGTGAHPVRSALRFS
                     DASV"
BASE COUNT          645 a         1597 c         1506 g          735 t
ORIGIN      
        1 ggcgaagtag atgttgggca cgaacgcggg gttgcccgcc tcgccgccgt tgccgcccaa
       61 gggcctctgc gtcgctgcca ccccggggaa tccggcggca gtggagccgc cgtcgctgaa
      121 cttcgtggac ggcttgatgt agtcgccgcc gagagaaagc tgtggccccg ccggcaggaa
      181 gctcattccc gcgggattga agtagatcgt gctcgcattc tcggcggtgg ccgcctgacc
      241 cgcgaacgcg ttgcccaggc cgctcgcgtt ctgctcctgg agcgcgaacg cggaggcgtg
      301 cgcctgcgaa tatccgacgg cgagcgcgcc tgcgaccgct acggacgcgg ccgtctttct
      361 gatccgggga tgtaccatgt tcaatctcct cctgacttga gatggcgtat ggggtggagt
      421 acggcttgtt ctatgcgccc gacacgatag catatcgtca tagggcgcag gcaacggcca
      481 ggcgccgtag tgcgcgagga tcagccaagc gtgactcggg cgaacttgcg cttgccgacc
      541 tgcaggacga cggtcttccc cgccgggatc tgcgtcccct tgtcggagac cttctcgccg
      601 tcgatccgga tcccgccctg ctcgatcatg cgcagcgcct cggaggtgct cgcggtcagc
      661 cccgcggcct tgaggacctg ggcgatcgcc atgggcttgg cgcccgccgc caccctcacc
      721 tcgggcatgt cctcgggcag ggcaccctcg cggaaccgcg cctcgaagtc ggcctgcgcg
      781 cgctcggctt cgcgctcgct gtggaagcgc gccacgatct cgcgggcgaa gcggaccttg
      841 acgtcgcggg ggttgagtcc ggattcgacc tcgcgcttcc agcgctccac ggtctcggcc
      901 ggctcgaacg acagcagctc aatgtagcgc cacatcaggt cgtcggagat cgacatgagc
      961 ttgccgaaga tggtctcggc cggctcgctg atgccgacat agttgccgag cgacttcgac
     1021 atcttgtcct tgccgtcggt cccctcgagc agcggcatca tcaggatcga ctgctgcggc
     1081 tggccgtggt gcttctgcag ctcgcggccc accagcaggt tgaacttctg gtcggtgccg
     1141 cccagctcga cgtcggattt catcgccacc gagtcgtagc cctgcatgag cgggtagagg
     1201 aactcgtgga cggcgatcgg cttgctgtcg tggaagcgct tggcgaagtc gtcccgctcg
     1261 agcatgcgcg cgacggtgta cgtggctgcg agcttcacca tcccggccgc gcccagctcg
     1321 tcgcaccatt tggagttgaa cagcacctcg gtgcgcttcg cgtccaggat cttgaacacc
     1381 tggtcctggt aggtggccgc gttccggagg atctgctccc tcgacagcgg cggtcgcgtg
     1441 acatttttcc cgctcgggtc cccgatcatt ccggtaaagt ccccgatcag gaactgaaca
     1501 tgatgaccga gctcctggaa ctggcgcagc ttgttgatca ccactgtgtg cccgagatgc
     1561 aggtcgggcg cggtcggatc catgcccaat ttcacgcgca gcgggcgccc ggctttcagc
     1621 ttgtcgcgca gctcctcctc gacgatgagc tcggacgctc cgcgcttcag gattccgagt
     1681 tggcggtccg cgtcgctcat gccgcgaagg tggtcggtct catggttcct tgctagactt
     1741 tcggcggatt cgagcgcggc catgaacgaa gggaagacgc gaattgtaac gcaaaggtat
     1801 ctgcaggcgg cccgccggcc gtggctcgcg gccgccttcg ccgtccccgt cttcggtgtc
     1861 gtcgccgcgt tcgcgaccgt gcagcccaac cccgagccgg tgctgacccg cgcggtggtc
     1921 gaggcgctgc cgctttccgc cgagatcgcg gtgcagggtt cggcgacccc gtacttccac
     1981 gaggagcgct tccagcgcgg cgacaccgtc acggcggtgc tggcgcgcct cggcgcggac
     2041 ggcgacgatg cgcgcgcgct gctcgcctcg cccggcgccc ggcgcctcgt gcgcctgatg
     2101 cggcccggca cgacggtgca ggccacgacg caggacgcga agctgcagtc gatgtggttc
     2161 ctgtccggga ccgattcgct gacgatcgtc gagcgcaagg acggcgcgtt cacggcctcc
     2221 gagcgcacgg tgagcctgtc gcgcgaagtg gagctgcgca ccgggcagat cgcgagctcg
     2281 ctgttcgcgg cgaccgacgc cgcgggcgtt cccgacaacg tggcgtcgca gatcgccgat
     2341 atcttcgcag gcgacgtcga cttccagcgc gacctgcgcc ggggcgaccg cttcaccgtt
     2401 gcgttcgaga tgttctacca cgagggacgc ccgatccggt cgggccgcct gctcgccgcc
     2461 gatttcacca accagcggca cacctaccgc gcgatctggt tccagcccgc cgacggtccg
     2521 ggcggctact actcgcccga cggcaagaac ctgcgcaagg ccttcctgcg ctcgccgctc
     2581 gagtactcgc gcatcagctc gggcttcggc atgcgcaatc acccgatcct gcagcagtgg
     2641 cgcgcgcaca agggcgtcga ctacgccgct ccgatcggga cccgggtgcg cgcgacgggc
     2701 gacggcgtgg tcgatttcgc cggcaagcgc aacggctacg gcaacgtcat cgtgctgcgg
     2761 cacaacggcg gctacagcac gtactacgcg cacctgaacg gcttcgcccg cggcgtgcaa
     2821 cgcggcgtgc gtgtcgcgca gggcgacatc atcggctacg tggggcagtc gggctgggcg
     2881 accgggccgc acctgcacta cgagttccac gttcgcaacg agtaccgcaa cccgctcacg
     2941 ctcgcgatgc cggcgtcgaa cccggtgccg caccatctgc tgattgcgtt cttccaggcc
     3001 tcacagcccc tcgccgccca gctcgacctg ctcaagaaca ccgacgtcgc gctgctcgag
     3061 taggttattc ttcccgagtg ttgactcggc gcggccgcgc agccggccgc ccgcggtagc
     3121 ccatgggcga gaagaagcga cgacaggccg ccgggaggcc gagcgcgcat gacgcacgct
     3181 cgacgatttc gcgcgcgctc gagttgatgc gctcgggtga cggcgaagcg gcgctcgcgc
     3241 tgtgcgagcc gctgttcgac gaacccctct ccgatcccga agccctgcac tacctgggct
     3301 acctcgcgat cgaactcgga cgcgccgggc gcgcggtcgg cgtgctgcag cgggcgatcc
     3361 ggcagcagcc cgccgatgcc cacctgcaca acacgctggc gatcgccttc cagaggctgg
     3421 ggcagccgga ttcggcggtc gaaacgctcg agcgtgcgat cgcgctcgcg cccggagtcc
     3481 acgagctcta caacaacctc ggtaactcgc tgtcggcgct cggacgtcac gccgaggcgg
     3541 cggcaagcta tctgcaggcc agccgcatcg caccggaaat cggcgcctac cgctccgctt
     3601 tcgccgaagc gctgtcgcac gtacgcggcg tcccggatcc gagcctgcgt ccggacctgc
     3661 tgcaggccct cgcgcacgag caggtcgatc cgggcaccct caccgacacc gtcaacaccc
     3721 tgctgcgcac cgacccgggt ttcgtcgagc tcgagcggct tgcagcagcg ggcgggctct
     3781 ccgcagcatc gctcgccgcg ccgccgccct tcctgcgcga cgagctgctg ctgcgtgcgc
     3841 tcggcgagac catcctcggc gatcccggga tcgagcgcct gctgacggga ctgcggcgcg
     3901 tcgcgctcga atgcgaggtc cgcggggagc cgtgccccgc ggtgccgacc gagctcgtgt
     3961 gcggcctcgc gctgcaatgc ttcgcgaccg aatacgcctg gtgggtaacc ccggaggaag
     4021 agcagctgct gcccgcgctc cgcgcgaagc tgttcccggc gccggcggac gcgagcccgt
     4081 cctgggagcg cgccgtggcg ctgcttgcct gctacgtcgc gctgcacgaa ttgccggagg
     4141 cgggcgagct ccagccgcgc ggcggaccgc tcgagcccgt cctgcgcagg caggtgttcg
     4201 agccccgcga agaggcgcgc ttgcggccgc tgatcccggg gctcgatccg atcgacgacg
     4261 cggtctcgca cgccgtccga aaccagtacg agacgcaccc gtacccgcgc tggacccgtc
     4321 tagggcgctt cgatcacgcc gcgccgctag gcgtggtcct gagggagctg ttcccgcgca
     4381 agaagctcgc cgccccagac ggcccgctgc agatcctggt cgcaggctgc ggcaccggcg
     4441 cgcacccggt gcgctcggcg ctccggttca gcgacgcctc cgt
//
LOCUS       DATDMW010000233         2222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_320043, whole genome shotgun sequence.
ACCESSION   DATDMW010000233 DATDMW010000000
VERSION     DATDMW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320043"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VI319_09905"
     CDS             <1..456
                     /locus_tag="VI319_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEY6864203.1"
                     /translation="QGLDEPGQIALFDLYNALRASGGALAAAGDEPPDGPRLRSRLRE
                     DLRSRLGSGVVIQLHPLSETEKAAALRERARARGIALSDGVIAYLLTHLRRDMGTQIA
                     VLDALDRHSLEHQRPITVPLLKQALRSLEQEKKGRRLPPDIAGPRKGGP"
     gene            complement(513..782)
                     /locus_tag="VI319_09910"
     CDS             complement(513..782)
                     /locus_tag="VI319_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864204.1"
                     /translation="MFDVLVVCGELASVALLSYGAYLVLGNFDIVRGRVASRLADDDT
                     RFLRSPVLQEPATAGAWPPSRARSFPGAAGGEPAVAGGFFSGQAE"
     gene            962..1624
                     /locus_tag="VI319_09915"
     CDS             962..1624
                     /locus_tag="VI319_09915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY6864205.1"
                     /translation="MDLALFDLDNTLLAGDSDYAWAQFLIGRGVLDRARYEARNDEFF
                     RQYKAGTLDIREFLEFQLAPLARRPRAELDAWHRDFMREHVVPMILPAARALIARHRG
                     RGDVCAILTSTNAFITAPIARELGIDHLLATELETCEEGYTGRPAGTPCFREGKIERL
                     EHWLGARGETLASYARSWFYSDSLNDLPLLERVTDPVAVDPDDTLLRVAVERGWPVMS
                     LK"
     gene            1621..>2222
                     /locus_tag="VI319_09920"
     CDS             1621..>2222
                     /locus_tag="VI319_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY6864206.1"
                     /translation="MSPETRVLPAAIVVLVPVALGLAIALGSVPMSAGQLAQALAGRG
                     DDALREIVWDVRVPRALAAFACGALLALSGALLQVLLRNPLADPYILGVSGGASVGAL
                     LALVLGLAAAAVRLFALAGAALAAGAVFAFSRGRAGWNPYRVLLTGVALSAGCGAIVG
                     LLLSLAPAAQVQGMLYWLLGDLSPAGDPAPALLVLAACTAL"
BASE COUNT          296 a          838 c          775 g          313 t
ORIGIN      
        1 cagggtctcg acgagcccgg gcagatcgcg ctgttcgacc tctacaacgc gctgcgcgcc
       61 tcgggcggag cgctcgcggc ggcgggcgac gagccgcccg acgggcctcg actgcgttcg
      121 cgcctgcgcg aggacctgcg ctcgcgactc ggcagcggcg tcgtgatcca gctgcacccg
      181 ctgtcggaaa cggagaaggc cgcggcgctg cgcgagcgcg ccagggcgcg cggcatcgcg
      241 ctctccgacg gcgtgatcgc gtacctgctc acgcacctgc ggcgcgacat gggcacgcag
      301 atcgcggtgc tcgacgcgct cgaccgccat tcgctcgagc accagcgccc gatcaccgtg
      361 cccctgctca agcaggcgct gcgctcgctg gagcaggaaa aaaaaggccg gcgactcccc
      421 ccggacatcg ccggcccccg caagggcggt ccgtagctcg ggcccggacc gccccccggc
      481 ccagtttcag aacgccgtgc tcatgacggc gctcactcgg cttgtccgct aaagaaaccc
      541 ccggcgaccg cgggctcacc gcccgcggcg ccagggaaag agcgagcgcg cgaaggaggc
      601 cacgcgcccg ctgtcgccgg ctcctgcaga accggcgatc ggaggaaccg tgtgtcgtcg
      661 tccgcgagcc gggacgcaac cctgccgcgg acgatgtcga aattgccgag caccagatac
      721 gcgccgtaag acagcagcgc gacgctcgca agctcaccgc agaccaccag gacgtcgaac
      781 atgttcttct cctttcgctc cgagacggaa atcggtgtcc gtcagagcga aatttaggcg
      841 gcgagcggcc cggcgctcta tgaaagccgg cactgggccg ctgtcggata gtggcggcgc
      901 cgcagtgccg gcgcgcacac gccggccgtg aaatactccg cacgacggta gaatcgcgcc
      961 ggtggacctc gccctcttcg acctcgacaa cacgctgctc gcgggcgaca gcgactacgc
     1021 ctgggcgcag ttcctgatcg ggcgcggcgt gctggaccgc gcgcgctacg aggcgcgcaa
     1081 cgacgagttt ttccggcaat acaaggccgg gacgctcgac atccgggagt tcctcgagtt
     1141 ccagctcgcc ccgctcgcgc gccggccgcg cgccgagctc gacgcctggc accgcgattt
     1201 catgcgcgag cacgtcgtcc cgatgatcct gcccgcggcg cgcgcgctga tcgcgcgcca
     1261 ccgcgggcgc ggcgacgtgt gcgccatcct cacctcgacc aacgccttca tcaccgcgcc
     1321 gatcgcgcgc gagctcggca tcgatcacct gctcgcgacc gagctcgaga cctgcgagga
     1381 aggctatacc ggccgcccgg ccggcacgcc gtgcttccgc gagggcaaga tcgagcggct
     1441 cgagcattgg ctcggggccc gcggcgagac gctcgcctcg tacgcgcgca gctggttcta
     1501 cagcgactcg ctgaacgacc tgccgctgct cgagcgcgtc accgatcccg tcgcggtcga
     1561 cccggacgac acgctgctgc gcgtcgcggt ggagcgcggc tggccggtga tgagcctgaa
     1621 atgagccctg aaacgagagt ccttcccgcc gcgatcgtcg tcctggtccc ggtcgctctc
     1681 ggcctcgcga tcgcgctcgg cagcgtcccc atgagcgcgg gccagctcgc ccaggcgctc
     1741 gccgggcgcg gcgacgacgc gctgcgcgag atcgtgtggg acgtgcgcgt gccgcgcgcg
     1801 ctggcggcgt tcgcgtgcgg cgcgctgctc gcgctgtcgg gcgcgctgct gcaggtgctg
     1861 ctgcgcaacc cgctcgccga tccctacatc ctcggcgtct cgggcggggc ctcggtgggc
     1921 gcgctgctcg cactggtgct ggggctcgcg gcggccgcgg tgcgcctgtt cgcgctcgcc
     1981 ggcgcggcgc tcgcggcagg cgcggtgttc gctttctcgc gcgggcgcgc cggatggaat
     2041 ccctaccgcg tgctgctcac cggcgtcgcg ctgtcggcgg gctgcggcgc gatcgtcggc
     2101 ctgctcctca gcctcgcgcc cgccgcccag gtgcagggca tgctgtactg gctgctcggc
     2161 gatctctcgc ccgcgggcga cccggccccg gcgctcctcg tgctcgcggc gtgcaccgcg
     2221 ct
//
LOCUS       DATDMW010000234         5621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1939784, whole genome shotgun sequence.
ACCESSION   DATDMW010000234 DATDMW010000000
VERSION     DATDMW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939784"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VI319_09925"
     CDS             complement(<1..326)
                     /locus_tag="VI319_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6864207.1"
                     /translation="MSRVAARLAPSLPPGARLAAGLSGGVDSVVLLSVLGELAPVLRF
                     SLSAIHVNHGISPNAPRWAEFCAGLCRAMDVPLQVETVDLSPWRALGLEGAARRARYD
                     AFARVQA"
     gene            complement(331..1299)
                     /locus_tag="VI319_09930"
     CDS             complement(331..1299)
                     /locus_tag="VI319_09930"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY6864208.1"
                     /translation="MKTTFLDFEQSIADLETKIEELRYVQDDSAVDISDEIQRLQKKS
                     AALARDIYAKLTPWQTSQVARHPQRPYTLDYIAAMFTDFEELHGDRSFADDASIVGGL
                     ARLNGQSVMVMGHQKGRDTKEKILRNFGMPRPEGYRKAMRLMRLAEKFGVPVITFVDT
                     PGAYPGIGAEERGQSEAIGRNLYEMAGLRTPVVVTVIGEGGSGGALAIAVGDVVQMLQ
                     YATYSVISPEGCASILWKSADKAPEAAETLGITASRLKTLGLIDRILNEPLGGAHRDP
                     AAMALTIKKSLQDALRALAEKDITTLVEERFERLMSYGKYKEAELE"
     gene            complement(1327..1968)
                     /locus_tag="VI319_09935"
     CDS             complement(1327..1968)
                     /locus_tag="VI319_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY6864209.1"
                     /translation="MSAAPGPDAAPGYWLRAKRALSKSDPVMAGIIRSHPRIFLRPRG
                     EPFTTLARAIVGQQISVKAAQSVWDRFAACVGEVTPERVLAKARPQLRACGLSDRKTE
                     YIADLAQHVADGVIHVHRWPQMDDEAIIGELVEVRGIGRWTAEMFLIFNLLRPDVLPL
                     DDLGLQRAICLHYFKGRPRSRRTMTRLASAWAPWRSVATWYLWRSLDPLPVEY"
     gene            complement(1965..2975)
                     /locus_tag="VI319_09940"
     CDS             complement(1965..2975)
                     /locus_tag="VI319_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HEY6864210.1"
                     /translation="MPDFWRNSGFHLLERDADGRLRVTDDFLRAYLLRPEIRPVDESG
                     PHEIALHDSLMDDPRRDVGESELARIEDVDARDNYRVLLRFLDRLKRSASLEACYAGL
                     FAGGNVDAPPLFLDQLAQIILRNVLDGCDDALEARAAEVFYREQKANTDGGVVMLADL
                     ETVEMHASGGTYGSLGRLIVEAQTPLSTVNLDVLDTENAAMYWLRDQRHDFVISLNFG
                     RAASKAFCRAVEKWIRHFFDVEVRVEALRAIEDRRWAWHVGLDAESTAILNDLWRGEE
                     VEPGRMRRVLALFRMDFADAGVVRPDVAGKPVYLALSMDEASVVRMKPQNLLLNLPLL
                     QA"
     gene            complement(2996..3583)
                     /locus_tag="VI319_09945"
     CDS             complement(2996..3583)
                     /locus_tag="VI319_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY6864211.1"
                     /translation="MPGEVPLILTLDNHGVPHRWVTWQQACFYYAKNLVAWTLGAESF
                     TFYGGVSRATGERSSITAHSIIAIKGRAMAMKGFNQVPPLNNRELFRRDRHVCAYCGG
                     EFSFLRLTRDHIRPVSRGGGDHWMNVVTACRACNGIKRNRLPEEAGLELLYAPYVPNK
                     AEYLILTNRKILADQMEFLVQHVSHHSRVARLAAA"
     gene            complement(3680..4528)
                     /locus_tag="VI319_09950"
     CDS             complement(3680..4528)
                     /locus_tag="VI319_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6864212.1"
                     /translation="MSGKDVTDASFESDVIEASRRLPVVVDFWAPWCAPCRALKPVLE
                     KLAAEHEGEFVLAKLNTDENPDTAARFGIRGIPAVKAFRDGKVAAEFTGAVPESSARA
                     FLGRVLPTPGDKLRVAASAAIAAGDPQAAEARLREALHAEPALQAARVDLAELLIARE
                     AWPDAEALLSELPEHERDDRAQNLASRVELWRAARELPDAAELRRRLDAAPGDPDARL
                     RLAERLAADGDFEAALEQLLDVVRAVRGPARDTARRTMLRVLVLAAGDSDLVARYRRL
                     LASALN"
     gene            complement(4563..5306)
                     /locus_tag="VI319_09955"
     CDS             complement(4563..5306)
                     /locus_tag="VI319_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY6864213.1"
                     /translation="MDWLLDPQAWIALATLTALEIVLGVDNIIFISILVGRLPDRERQ
                     RARVVGLALAMLTRILLLLTLAWIMTLTRPLLEVAGRGVSGRDLILFCGGLFLLWKSV
                     HEIHNSLEGEADSGGGAKRAGFAAVIAQIAVIDIVFSLDSVITAVGMVDQVPVMVSAI
                     VLAVAVMMFAARPIGDFVDRHPTIKMLALSFLILVGFALVAEAFNVHVPKGYIYFAMA
                     FSVGVEMLNLRVRARRGEPVRLHRGGPDP"
     gene            complement(5414..5490)
                     /locus_tag="VI319_09960"
     tRNA            complement(5414..5490)
                     /locus_tag="VI319_09960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5454..5456),aa:Arg,seq:acg)
BASE COUNT          883 a         1960 c         1953 g          825 t
ORIGIN      
        1 gcctggacgc gcgcgaacgc atcgtagcgg gcgcgccgcg ccgcgccctc gaggccgagc
       61 gcgcgccagg gcgagaggtc gaccgtctcg acctgcaggg gaacgtccat cgcgcggcag
      121 agcccggcgc agaactccgc ccagcgcggc gcgttggggc tgatgccgtg gttgacgtgg
      181 atcgcagaga gcgagaagcg cagcacgggc gcgagctcgc cgagaaccga cagcaggacc
      241 accgaatcga cgccgccaga aagcccggcc gcgagccgcg cgcccggcgg cagggacggc
      301 gccagacgcg ccgcgacgcg gctcacgagc tcattcgagc tcggcttcct tgtatttgcc
      361 atagctcatc aggcgctcga agcgctcctc gacgagcgtc gtgatgtcct tctcggcgag
      421 cgcgcgcagc gcgtcctgca gcgacttctt gatggtgagc gccatcgccg cggggtcccg
      481 gtgcgcgccg ccgagcggct cgttcaggat gcggtcgatg agcccgagcg tcttcaggcg
      541 gctcgcggtg atgccgagcg tctcggcggc ctcgggcgcc ttgtcggcgc tcttccacag
      601 gatggacgcg cagccctcgg gcgagatcac cgagtaggtc gcgtactgca gcatctgcac
      661 cacgtcgccg accgcgatcg cgagcgcgcc gcccgagccg ccttcgccga tcaccgtcac
      721 gaccaccggg gtgcgcagcc cggccatctc gtagagattg cgaccgatgg cctcggactg
      781 gccgcgctcc tcggcgccga tgccgggata cgcgcccggc gtgtcgacga aggtgatcac
      841 cgggacgccg aatttctcgg cgagccgcat cagccgcatc gccttgcggt agccctcggg
      901 ccgcggcatg ccgaagttgc gcaggatctt ctccttggtg tcgcgcccct tctggtggcc
      961 catcaccatc accgactgcc cgttcaggcg cgccagcccg cccacgatcg aagcgtcgtc
     1021 ggcgaagctg cggtcgccgt gcagctcctc gaagtcggtg aacatcgcgg cgatgtagtc
     1081 gagcgtgtag ggccgctgcg ggtggcgcgc gacctgcgag gtctgccacg gcgtgagctt
     1141 cgcgtagatg tcgcgcgcga gcgcggcgct cttcttctgc aggcgctgga tctcgtccga
     1201 gatgtcgacg gccgagtcgt cctggacgta gcgcagctcc tcgatcttgg tctcgaggtc
     1261 cgcgatcgac tgctcgaagt cgagaaaggt ggttttcatg gcggccggac cgccgccatt
     1321 ctactctcaa tactcgaccg gaagagggtc caggctgcgc cagagatacc aggtcgccac
     1381 cgagcgccac ggcgcccatg cgctcgcgag ccgggtcatc gtccggcgcg atcgcggccg
     1441 gcccttgaaa tagtgaaggc agatcgcgcg ctgcagcccg aggtcgtcga gcggcagcac
     1501 gtcgggccgc agcaggttga agatgaggaa catctcggcg gtccagcggc cgatgccgcg
     1561 cacctcgacc agctcgccga tgatcgcctc gtcgtccatc tgcggccagc ggtgcacgtg
     1621 gatgacgcca tcggcgacat gctgcgcgag gtcggcgatg tactcggtct tgcggtcgga
     1681 gaggccgcac gcgcgcagct gcggcctcgc cttcgcgagc acgcgctccg gcgtgacctc
     1741 gccgacgcag gcggcgaacc ggtcccagac gctctgcgcg gccttcaccg agatctgctg
     1801 gccgacgatc gcgcgcgcga gcgtcgtgaa cggctcgccc cgcgggcgca ggaagatcct
     1861 cggatgcgag cggatgatcc ccgccatcac cggatcggat ttcgagagcg cgcgcttcgc
     1921 gcgcagccag tacccgggcg ccgcgtccgg tccgggcgcc gcgctcatgc ctggaggagc
     1981 ggcaggttca gcagaagatt ctgcggcttc atccggacga cgctcgcttc gtccatcgac
     2041 agcgccaggt acaccggctt cccggcgacg tccggccgca cgacgcccgc gtcggcgaag
     2101 tccatgcgaa acagcgcgag cacgcggcgc attcgtccgg gctcgacttc ctcgccgcgc
     2161 cagagatcgt tgaggatcgc ggtcgactcc gcgtccagcc cgacgtgcca cgcccagcgc
     2221 cgatcctcga tcgcgcgcag cgcttcgacg cgcacctcga catcgaagaa atgccggatc
     2281 catttctcga cggcgcggca gaaggccttg ctcgcggcgc gcccgaagtt caggctgatc
     2341 acgaaatcgt ggcgctgatc ccgcagccag tacatcgccg cgttctccgt gtcgagcacg
     2401 tcgagattga ccgtcgagag cggcgtctgc gcctcgacga tgaggcgccc caggctcccg
     2461 tacgtgccgc ccgacgcgtg catctcgacc gtctcgagat cggcgagcat cacgacgccg
     2521 ccgtcggtgt tcgccttctg ctcgcggtag aacacttcgg cggcgcgcgc ttcgagcgcg
     2581 tcgtcgcagc cgtcgagaac gttgcgcaga atgatctgcg ccagctgatc gagaaagagc
     2641 ggcggcgcgt cgacgttgcc gccggcgaag aggccggcgt agcaggcctc gagcgacgcg
     2701 gaccgcttca gccggtcgag gaaccggagc aagacgcggt agttgtcgcg cgcgtccacg
     2761 tcctcgatgc gcgcgagctc gctctcgccc acgtcgcgcc gcggatcatc catgagtgaa
     2821 tcgtggagcg cgatctcgtg cggccccgat tcgtcgaccg ggcggatctc cggccgcagc
     2881 aggtacgcgc gcaggaagtc gtcggtgacg cgcagacgtc cgtcggcgtc gcgctcgaga
     2941 aggtgaaacc cggagttgcg ccagaagtcc ggcaaagaag ccgacgagcg gcggctcagg
     3001 cggccgcgag cctcgccacg cggctgtggt gcgagacgtg ctggaccagg aactccatct
     3061 ggtcggcgag gatctttcga ttcgtgagga tcaggtactc ggccttgttg ggcacgtagg
     3121 gggcgtagag cagctcgagg cccgcctcct cgggcaggcg gttgcgcttg atgccgttgc
     3181 acgcgcggca ggccgtcacc acgttcatcc agtggtcgcc cccgccgcgc gacaccggcc
     3241 ggatgtggtc gcgcgtcagg cgcaggaacg agaactcgcc gccgcaatac gcgcagacgt
     3301 gccggtcgcg ccggaacagc tcgcggttgt tgagcggcgg aacctggttg aagcccttca
     3361 tcgccatcgc gcggcccttg atcgcgatga tcgagtgcgc ggtgatactg gagcgctcgc
     3421 cggtggcgcg cgagacgccg ccgtagaaag tgaacgactc cgcgcccagg gtccacgcga
     3481 ccaggttctt cgcgtagtag aagcaggcct gctgccacgt gacccagcga tgcggcacgc
     3541 cgtgattgtc gagcgtcaga atcagcggta cttcacccgg catgcgatgt ctccggcact
     3601 gtcctcgtcg tttttgactg caaagattgt gccgtaaaag cgcgcgccgc gccatccccc
     3661 gcgcccgggt cgccgtgaat cagttcaagg cgctcgcgag cagccgccgg tagcgcgcga
     3721 cgaggtcgga atcgcccgcg gcgagcacga gcacgcgcag catggtcctg cgcgccgtgt
     3781 cgcgcgccgg cccgcggacg gcgcgcacga cgtcgaggag ctgctcgagc gcggcctcga
     3841 aatccccgtc cgcggcgagc cgctcggcga gacgcagcct cgcgtccgga tcgccgggcg
     3901 cggcgtcgag acgccggcgc agctcggcgg cgtccgggag ctcgcgcgcg gcgcgccaca
     3961 gctcgacccg gctcgcgaga ttctgcgcgc gatcgtcgcg ctcgtgctcg ggcagctcgg
     4021 agaggagcgc ctcggcgtcg ggccacgcct cgcgcgcgat cagcagctcg gcaagatcca
     4081 cgcgcgcggc ctggagcgcc ggctccgcgt gcaaagcctc gcgcagacgc gcctcggccg
     4141 cttgcgggtc tcccgccgcg atcgccgcgg aagcggcgac gcgcagcttg tcgcccggcg
     4201 tcggcaggac ccttcccagg aaggcgcgag ccgaggactc gggcacggcg ccggtgaact
     4261 ccgccgcgac cttgccgtcc cggaaggcct tcaccgcggg tattccgcgg atcccgaagc
     4321 gcgccgccgt gtccggattt tcgtcggtat tcagcttcgc gagcacgaac tcaccctcgt
     4381 gctcggcggc gagcttctcg aggacgggct tgagcgcccg gcacggcgcg caccagggcg
     4441 cccagaagtc gaccacgacc ggcaacctgc gcgaagcctc gatgacgtcg ctctcgaagc
     4501 tggcgtcggt gacgtccttg ccgctcatgg tggttccaac atgccgccgc acggcgcccg
     4561 tttcaagggt ccggaccgcc ccggtgcaga cgcaccggct cgccgcgccg ggcgcgcacc
     4621 cgcaggttga gcatctcgac cccgaccgag aacgccatcg cgaagtagat gtagcccttc
     4681 ggcacgtgga cgttgaacgc ctcggcgacc agcgcgaagc cgacgagaat gaggaacgac
     4741 agcgcgagca tcttgatggt cgggtgccgg tcgacgaagt cgccgatcgg ccgggcggcg
     4801 aacatcatga ccgcgaccgc gagcacgatg gccgacacca tcaccgggac ctggtcgacc
     4861 atgccgacgg cggtgatgac cgagtcgagc gagaacacga tgtcgatgac ggcgatctgc
     4921 gcgatcaccg cggcgaaccc ggcgcgcttc gccccgccgc ccgagtccgc ctcgccctcg
     4981 agcgagttgt ggatctcgtg cacgctcttc cacagcagga acagcccgcc gcagaacagg
     5041 atgaggtcgc gccccgacac gccgcggccg gcgacctcga gcagcggccg cgtcagggtc
     5101 atgatccagg cgagcgtcag cagcagcagg attcgggtca gcatcgcgag cgcgagcccg
     5161 acgacccgcg cgcgctggcg ctcccggtcg ggcaggcggc cgaccaggat gctgatgaag
     5221 atgatgttgt ccacgccgag gacgatctcg agcgcggtca gcgtcgcgag cgcgatccag
     5281 gcctgcgggt cgaggagcca gtccatcgtg gcctctgctc tagatggctt ccgcagctcg
     5341 ctgcagcggc ggacgattca agcgaaacgg cccggagcgc tgccgctccg ggccgtcgga
     5401 gaccgctgga atgtggcgcg cccggcagga tttgaaccca cgaccccttg gttcgtagcc
     5461 aagtactcta tccaactgag ctacgggcgc gaagcgcgga ttatatcaaa tcaccctagc
     5521 ggcacggcac cggccggagc gcgcagcggt cggggtaggt cgccacgtat ttcgtgcggc
     5581 cctgtggatc gaaatgcatc tcggcgacgt aggtgaacgt g
//
LOCUS       DATDMW010000235         2895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1691032, whole genome shotgun sequence.
ACCESSION   DATDMW010000235 DATDMW010000000
VERSION     DATDMW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691032"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VI319_09965"
     CDS             complement(<1..529)
                     /locus_tag="VI319_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6864214.1"
                     /translation="MDRISDSAAIRETASSPLAGAVVVITGASSGIGRAAAHAFAREG
                     ACVVLAARREGALEDAAAECAALGAASALAVPTDVADADAVERLAERAESAFGRIDVW
                     INNAGVALLGRVEEVPLADFRRVLDVNLFGYVHGARAALRRFRAQGRGVLINNASILG
                     LAGWPYASAYVASKFA"
     gene            complement(603..2060)
                     /locus_tag="VI319_09970"
     CDS             complement(603..2060)
                     /locus_tag="VI319_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6864215.1"
                     /translation="MPALSRPEQPRSVELPPRTGTLAAETDVLVVGGGPAGLGAAVGA
                     ADAGARVVLAERYGFLGGNATAALVMPLMSFHTQKHSREKTGATTLLPTDHGPGDPIV
                     AGVLKRLLERLVRSGGAIRPSLETGYVVPFDPEWFKICALDLLDEAGVQFLLHAFAGD
                     VVCEPGDGCRLSGVVFETKSGPMLVRARVIVDCTGDGDIAARAGAPFEIGRFDGLTQP
                     MTLMFRMVDFERAAFESYVRAHPKEWRGVHGLWDLVRKATAAGELELAREDILFFATP
                     HERELSVNTTRITRVLGVDVWDLSYAEWRGRQQMRQIAAFLRKYVPGFERAYVAQSGV
                     TVGVRETRRIAGDYVLTADDVLGARKFGDAIARGAYPVDIHNPEGKGTILRHLPPGEA
                     YDIPLRCLLPHAVDNLLVAGRCISGTHEAHSSYRVMPIVMATGQAAGVCAALAARASR
                     APRAVPAREVQRELVRQGASLRKDVLAGAELAEHS"
     gene            complement(2066..>2895)
                     /locus_tag="VI319_09975"
     CDS             complement(2066..>2895)
                     /locus_tag="VI319_09975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY6864216.1"
                     /translation="AGIPVMLYAIGAGPLKDPAAQVLVRDCLEPVELVTVRERRAGKI
                     LEDAGVNRDIVVTADPALLLEPEPLPEGALAQEGLDGSKNLIGMSVREPGVAAPGLDE
                     EVYHALLANAADFMADRFDAHIVFVPMEHSVLDTQHSHAVISRMLRAQRATVLKGEYT
                     PGQVLSLMGHFSFALGMRLHFLIFAALQGVPFVALPYASKVSGFLEDLQLTAPPLKLV
                     NAGRLIAYLDQCWDGRREMRERISEELPALKERAKETHRLAMELLARREPRREPQAA"
BASE COUNT          408 a         1046 c         1065 g          376 t
ORIGIN      
        1 tcgcgaattt gctcgcgacg taggcgctcg cgtaaggcca tccggcgagc ccgagtatcg
       61 acgcgttgtt gatgagcacg ccccggccct gcgcgcggaa acggcgcagc gccgcgcgcg
      121 cgccgtgcac gtagccgaac agattcacgt cgagcacgcg cctgaagtcc gcgagcggca
      181 cctcctcgac gcggccgagg agcgcgaccc cggcgttgtt gatccacacg tcaatgcgcc
      241 cgaaggcgct ctccgcgcgc tcggcgagac gctcgacggc gtctgcgtcg gcgacgtcgg
      301 tgggaacggc gagcgcggag gcagcgccga gcgcggcgca ctcggcagcc gcgtcttcga
      361 gcgcgccttc gcgccgcgcg gcgagcacga cgcaggcgcc ctcgcgcgcg aaagcgtggg
      421 cggcggcgcg gccgatgccg ctcgacgcgc cggtgatgac gacgacggcg cccgcgagcg
      481 gcgagcttgc ggtctcgcga atggcggcgc tgtccgatat gcgatccatg gcttcccctt
      541 ccgtgcttgg actgcagttc cggcgcgatg gctcgctcaa tgcgccgacg ggaccacgcc
      601 gctcaggaat gctcggcgag ctcggcgccg gcgaggacgt ccttgcgcag gctcgcgccc
      661 tggcgcacga gctcgcgctg gacctcgcgc gccgggacgg cgcgcggcgc gcgcgacgcc
      721 cgggccgcga gcgcggcgca gacgccggcg gcctgcccgg tggccatgac gatcggcatc
      781 acgcggtagg acgagtgcgc ctcgtgcgtg cccgagatgc agcggcccgc gaccagcagg
      841 ttgtcgaccg cgtgcggcaa gaggcacctc agcgggatgt cgtaggcctc gcccggcggc
      901 aggtgcctga ggatcgtgcc cttgccctcg gggttgtgga tgtcgacggg atacgcgccg
      961 cgcgcgatcg cgtcgccgaa cttgcgcgcg ccgagcacgt cgtccgcggt gagcacgtag
     1021 tcgccggcga tgcggcgcgt ctcgcgcacg ccgaccgtga cgccgctctg ggcgacgtag
     1081 gcgcgctcga agccgggaac gtacttgcgc aggaacgcgg cgatctggcg catctgctgg
     1141 cggccgcgcc attcggcgta agagaggtcc cacacgtcga cgcctagcac tcgcgtgatg
     1201 cgcgtcgtgt tgaccgagag ctcgcgctcg tgcggcgtgg cgaagaagag aatgtcctcg
     1261 cgcgcgagct cgagctcccc cgcggccgtc gccttgcgca ccaggtccca cagcccgtgc
     1321 acgccgcgcc attccttggg atgcgcgcgc acgtaggact cgaacgccgc gcgctcgaag
     1381 tcgaccatgc ggaacatcag cgtcatcggc tgcgtcaggc cgtcgaagcg cccgatctcg
     1441 aagggcgctc ccgcccgcgc cgcgatgtcg ccgtcgccgg tgcagtcgac gatcacgcgg
     1501 gcgcggacga gcatcggccc ggacttggtc tcgaacacca cgcccgagag ccggcagccg
     1561 tcgccgggct cgcacaccac gtcgccggcg aacgcgtgca gcaggaactg cacccccgcc
     1621 tcgtcgagga gatcgagcgc gcagatcttg aaccactcgg ggtcgaaggg caccacgtag
     1681 ccggtctcga gcgagggccg gatcgcgcct cccgagcgca ccagccgctc gagcaggcgc
     1741 ttcagcacgc ccgcgacgat ggggtcgccc ggcccgtggt cggtcggaag cagcgtcgtg
     1801 gcgccggtct tctcgcgcga atgcttctgc gtgtggaacg acatgagcgg catcacgagg
     1861 gcggcggtcg cgttgccgcc gagaaagccg tagcgctcgg cgagcacgac acgcgcgccg
     1921 gcgtcggcgg cgccgaccgc cgcgcccagt cccgcgggcc cgccgcccac gacgagcacg
     1981 tcggtctcgg cggcgagcgt cccggtgcga ggcggcagct cgaccgagcg cggctgctcg
     2041 gggcgggaca gcgcgggcac ggagctcagg ccgcctgcgg ctcccggcgc ggctcgcggc
     2101 gcgcgagcag ctccatcgcc aggcggtgcg tctccttcgc gcgctccttg agcgcgggca
     2161 gctcctcgga gatgcgctcg cgcatctcgc gccggccgtc ccagcactgg tcgagatagg
     2221 cgatgaggcg cccggcgttc acgagcttga gcggcggcgc cgtgagctgc aggtcctcga
     2281 ggaagcccga caccttgctc gcgtagggca gcgcgacgaa gggcacgccc tggagcgcgg
     2341 cgaagatcag gaagtgcagc cgcatcccga gcgcgaagct gaagtgcccc atcagcgaca
     2401 gcacctgtcc cggggtgtac tcgcccttca gcacggtggc ccgctgcgcg cgcagcatgc
     2461 gcgagatcac ggcgtggctg tgctgggtgt cgagcacgga gtgctccatc ggcacgaaca
     2521 cgatgtgcgc gtcgaagcga tcggccatga aatcggccgc attcgcgagc agcgcgtggt
     2581 agacctcctc gtcgaggccg ggcgccgcga cgccgggctc gcgcaccgac atgccgatca
     2641 ggttcttcga gccgtccagc ccctcctgcg cgagcgcgcc ctcgggcagc ggctcgggct
     2701 cgagcagcag cgccgggtcg gcggtcacga cgatgtcgcg gttgaccccc gcgtcctcga
     2761 ggatcttgcc ggcgcgccgc tcgcgcaccg tcacgagctc gaccggctcg aggcagtcgc
     2821 gcacgaggac ctgcgccgcc gggtccttca gcggcccggc gccgatggcg tagagcatga
     2881 ccgggatgcc ggcct
//
LOCUS       DATDMW010000236         8117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_694756, whole genome shotgun sequence.
ACCESSION   DATDMW010000236 DATDMW010000000
VERSION     DATDMW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694756"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VI319_09980"
     CDS             <1..247
                     /locus_tag="VI319_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoin utilization protein B"
                     /protein_id="HEY6864217.1"
                     /translation="PAKCEYFPLAKGDVETVRTAGGGGFGAPWERDAERVRADVLEGY
                     VTPEAARERYGVVLKGETLEIDAAATAKRRDAMRASR"
     gene            complement(244..567)
                     /locus_tag="VI319_09985"
     CDS             complement(244..567)
                     /locus_tag="VI319_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864218.1"
                     /translation="MKKIAILALAGAALAAACGLAQARVVFGIGIGVPIWGAPFYPYP
                     YGPYYDPYYYPRTVVVQPQQPQRYIEQSSPDSGYWYYCAESKAYYPYVKDCPGGWQRV
                     SPTPR"
     gene            complement(644..1210)
                     /gene="folE"
                     /locus_tag="VI319_09990"
     CDS             complement(644..1210)
                     /gene="folE"
                     /locus_tag="VI319_09990"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY6864219.1"
                     /translation="MEPNAKKIEALVRELLKELGEDPGREGLRDTPMRVAKSLAFLTH
                     GYRLPLEQVINNALFTQDTSSMVIVKDIELYSLCEHHMLPFYGRCHIGYIPNGKVFGV
                     SKLARMVDMYARRLQLQERLTEQISQQVMDSIGAKGVGVMIEARHLCMMMRGVEKQNS
                     TMVTSSVLGVFREYAATREEFMSLIGRR"
     gene            complement(1226..2038)
                     /locus_tag="VI319_09995"
     CDS             complement(1226..2038)
                     /locus_tag="VI319_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY6864220.1"
                     /translation="MAVYGLVADIHGNLEALEAALAALDARGVRRIVCLGDLVGYNAD
                     SDAVVRVLRERGAECIAGNHELIGIGRLGTGKCSDATAYALMRTRRTLAPGTRAFLAA
                     LPASRVLEGRFALVHGGVDDPEQYLRSSAAVLDNAARLARRFPGVPVCFFGHTHEPGV
                     FAVEDGAVRRIAGVDEARLAPGPLYFVNPGSVDAARKRDAPAGAVRCAEFAVLDSGAM
                     RVEFLRVPYDHAAAEAKALAGGYRMSRARARWMRLGRRVRSAASRLASQVGA"
     gene            complement(2017..3324)
                     /locus_tag="VI319_10000"
     CDS             complement(2017..3324)
                     /locus_tag="VI319_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864221.1"
                     /translation="MRIIRALDEMLLTRNPTGATHAAVPIPDPALRRRRTGSARPPAF
                     VLGGINLVRPLALAGIPVVLGTDDEEEPALRSRHVGETCRLPSPVTAPREAVDALLRA
                     GALLRYRVGARLPLVVGRDDWLGLLHRNRAAIERHFVFLANDPELGEALLDKSRFHAV
                     ALERGLPVPRRFDWTDEDALRRHAGPVIAKPRLKLHWSESPALRALNGRYGKGVLYPG
                     PDALLADERFAPFRELFLVQEYIPGSDEALVSYHGLTDEHGCVLGSFVGRKLRTSPPR
                     TGDSSLIELVRDDAAEAAGRRIVEALGLKGPFKLDLKRDERDGRIWLIEVNARFSLWN
                     YLGAVNGVNLLALAYEYLVEGARPRPTGYRTRTRWLDLALDYKAYRQLRAEGALSTRE
                     WLRSIVFARKVHSVFRWNDPWPACHRWLAWGRRKVGRWLSTAS"
     gene            complement(3416..5836)
                     /locus_tag="VI319_10005"
     CDS             complement(3416..5836)
                     /locus_tag="VI319_10005"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY6864222.1"
                     /translation="MKSRWLNRALRCLLAAAVAAGCCAALAQGGPVPAPKPDDKAKAA
                     AKPEPPRPEAVPLADAPRRAEDASNLLGDLREEAGDDSTARGVESDLRVLTREIDARL
                     VDNRRAVARTSSIETLRRIEARWAPLRRNLAELARRLAARIERLDREAAQVDDAAATW
                     AKTLEESRTEAPPEIVKRLEALAGEIARTRTTIGAQRARLLALQNRVATQDGRMADAI
                     AAARRAREDLEENIFERDSPPLWAAASAGPSFAEEARRSLASQASAVEAYAERQSERM
                     AAHALAFVALTAFLYWTRRRVRRWLKDEPRLADTGAIFDVPAATALLVTLAGGFRLYP
                     DAPVLLYASMGALVLAPAVIILRRLIERGMYPVLYALVAFYVVDQVRLIAAPLQLAPR
                     LIVFAEMVGAVVFSGWLLARSGPAARAAARDSPDQALRSGAILHWAGWAALALAIVSL
                     AANMLGYVGLANLLGNALLATAYAAVMLYALVEIADGLFVMALHLPPFTLLGAVKRHR
                     ALISRRVLRFMQWAAAGLLVLFALDRLGLRDRLFKSAQEILIAESRWGAIRVSLADLL
                     EFAVSVWAAFLLSRFVRFLLEEDVYTRFNMRRGMPYAISTMLNYVILIVGFLAGVAAL
                     GFDMTKFTILAGAFSIGVGFGLQNIFNNFVSGLILLFERPVKVGDVIQIEDSSGIVEH
                     IGIRASTVRTTTGSEIIVPNGKLISERVVNWTFSSHERSIQLPIAVPIGTDLPSVIRV
                     LQEVARQCEPIAKTPPPKAIVVRMGPDWLGLELHAWTERVDQWMEIRSDLAVAAAAAL
                     AQAKITVR"
     gene            complement(5934..7241)
                     /locus_tag="VI319_10010"
     CDS             complement(5934..7241)
                     /locus_tag="VI319_10010"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HEY6864223.1"
                     /translation="MARPLRFLLLAWALALGCAHAQDPEPPTLNLTIEQATQLATELL
                     EQGKLDPAERLIARLRAAPEPSLQVLFLSGHLKLARGDVAGAVEEFRRILNRDPTAVR
                     VRLDLALALYRERDYPAALYHFELALSTDLPPQAKANALRFVRDIRQRQSYWTLDVAI
                     VSDSNVNYATRATDVPIGEARFQLNEDARAKAGTGLMVTLGGRKSFGPGLDNYVNLYT
                     EDTNFKGHRFDLFYGALTVGHTFAFASHDVFFEAGGHYANYGERSLYDGTLAKLGDNW
                     RVRPDTRVTTTLEARELRYQDFGFLTGNQYALNLDWTRALHGSTLLRLGSSVIKQEAQ
                     ALSSAYDAQGLRGEVQTELASAWTVGMRAETYWFRYDAPDPLFGATRHDRRSLLEATL
                     LRRDLRWHGFSPKLIVGYNVGVSNIPLFAFDRSYVRLAFTRQF"
     gene            complement(7299..>8117)
                     /locus_tag="VI319_10015"
     CDS             complement(7299..>8117)
                     /locus_tag="VI319_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEY6864224.1"
                     /translation="DQGPNPQLAPLGQVAAVDHHIDFTQQTALTPAGLGSFDSLNDMP
                     APEVSLANTPVIHVAIPDLGIDDVFDGPVTTTMVNGLRVDTSSKSDATTRRTVTYLAP
                     DAASTNPLKLQFSSLGVWNTTDIASGQMTNAAAFSVGTRTLGTDIPTNGTATYNGAML
                     GVAVEGTSQYSVTGNASALADFRARSIGVQTSSSVRTDLATGASSAAPGYNMTGTLTY
                     PAATNALSGTMTTADGKTGTASGNFYGPQAAELGMTFNLKSQTGTLVGGAALKR"
BASE COUNT         1154 a         2789 c         3036 g         1138 t
ORIGIN      
        1 gccggcgaag tgcgagtatt tcccgctcgc gaaaggcgac gtcgagacgg tgcgcaccgc
       61 gggcggcggc ggcttcggcg cgccgtggga gcgcgacgcc gagcgcgtgc gcgccgacgt
      121 gctcgagggc tacgtcacgc cggaggccgc gcgcgagcgc tacggcgtgg tgctgaaggg
      181 cgagacgctc gagatcgatg ccgcggcgac ggcgaagcgg cgcgacgcga tgcgcgcgtc
      241 gcgctagcgc ggcgtcggcg agacgcgctg ccagccgccc gggcagtcct tgacgtacgg
      301 gtagtaggcc ttcgactcgg cgcagtagta ccaatagccg ctgtcgggcg aggactgctc
      361 gatgtagcgc tgcggctgct gcggctgtac gaccaccgtg cgcggatagt agtacgggtc
      421 gtaatacggg ccgtacggat aagggtagaa gggcgcgccc cagatcggca cgccgatgcc
      481 gatgccgaac acgacgcgcg cctgcgcgag cccgcaggcg gccgcgagcg ccgccccggc
      541 cagggcgaga atcgcgattt tcttcatggt cgttcctttg cgggatccgt gcgcgtcatt
      601 gtgcgccgaa cccggggcgc gagcggtatc gatttgtaac ggcctagcgg cgcccgatca
      661 gggacatgaa ctcctcgcgc gtggcggcgt actcgcggaa caccccgagc acggacgacg
      721 tcaccatggt cgagttctgc ttctcgaccc cgcgcatcat catgcacagg tggcgcgcct
      781 cgatcatcac gcccacgccc ttggcgccga tcgagtccat cacctgctgc gagatctgct
      841 cggtgagccg ctcctgcagc tgcaggcggc gcgcgtacat gtcgaccatg cgcgcgagct
      901 tcgagacgcc gaacaccttg ccgttcggga tgtagccgat gtggcagcgg ccgtagaacg
      961 gcagcatgtg gtgctcgcac aggctgtaga gctcgatgtc cttgacgatc accatgctgc
     1021 tcgtgtcctg cgtgaacagc gcgttgttga tcacctgctc gagcggcagc cggtagccgt
     1081 gcgtgaggaa ggcgagcgac ttggcgacgc gcatcggcgt gtcgcgcagg ccctcgcgcc
     1141 cggggtcttc gccgagctcc ttcagcagct cgcgcaccag cgcctcgatc ttcttggcat
     1201 tgggttccat cgctcgactc cgacgtcagg ctccgacctg ggacgcgagc cgcgacgcgg
     1261 cggagcgtac gcggcggccg aggcgcatcc aacgcgctcg cgcgcggctc atgcgatagc
     1321 cgcccgcgag cgccttcgcc tcggcggccg cgtggtcgta cggcacgcgg aggaactcga
     1381 cgcgcatcgc gccggagtct agcacggcga actcggcgca ccggacggcg ccggcgggcg
     1441 cgtcgcgctt gcgcgcggcg tcgaccgatc ccgggttcac gaaatacaac ggccccggcg
     1501 cgagccgcgc ttcgtcgacg ccggcgatgc gccgcaccgc gccgtcctcg accgcgaaca
     1561 cgccgggctc gtgcgtgtgg ccgaagaagc acacggggac gccgggaaaa cggcgcgcga
     1621 gtcgcgcggc gttgtcgagc accgcggcgg aggagcgcag gtactgctcg ggatcgtcga
     1681 cgccgccgtg cacgagcgcg aagcgcccct cgagcacgcg gctcgcgggc agcgcggcga
     1741 ggaaggcgcg cgtcccgggc gcgagcgtgc gccgcgtgcg catgagcgcg tacgcggtgg
     1801 cgtcgctgca cttgccggtc ccgagccggc cgatgccgat cagctcgtgg ttgccggcga
     1861 tgcactcggc gccgcgctcg cgcagcacgc gcaccaccgc gtcggagtcg gcgttgtagc
     1921 cgaccaggtc gccgaggcag acgatgcggc gcacgccgcg cgcgtcgagc gccgcgagcg
     1981 cggcctcgag cgcctcgaga ttgccgtgga tgtcggctac gaggccgtag acagccatcg
     2041 cccgactttg cggcgccccc acgcgagcca gcgatggcac gcgggccaag gatcgttcca
     2101 gcggaacacg gaatggacct tgcgcgcgaa gacgatcgag cgcagccact cgcgcgtgct
     2161 gagcgcgccc tcggccctga gctgccggta ggccttgtag tcgagcgcga ggtcgagcca
     2221 gcgcgtgcgc gtgcggtagc cggtggggcg ggggcgcgcg ccctcgacga ggtactcgta
     2281 agcgagcgcg agcaggttga cgccgttcac cgcgccgaga tagttccaca ggctgaagcg
     2341 cgcgttgacc tcgatcagcc agatcctgcc gtcgcgctcg tcgcgcttga ggtcgagctt
     2401 gaacgggccc ttcaggccga gcgcctcgac gatgcgccgg ccggccgcct cggccgcgtc
     2461 gtcgcgcacc agctcgatca gcgagctgtc gccggtgcgc ggcggcgagg tcctgagctt
     2521 gcgtcccacg aaggagccga gcacgcagcc gtgctcgtcg gtgaggccgt gatacgacac
     2581 gagcgcctcg tcgctccccg ggatgtactc ctgcaccagg aacagctcgc ggaacggcgc
     2641 gaaccgctcg tcggcgagca gcgcgtcggg cccggggtag aggacgccct tgccgtagcg
     2701 gccgttcagc gcgcgcagcg ccggcgactc gctccagtgc agcttgaggc gcggcttggc
     2761 gatcacgggc ccggcgtgcc ttcgcagcgc atcctcgtcg gtccagtcga agcgccgcgg
     2821 caccggcagc ccgcgctcga gcgcgaccgc gtggaagcgc gacttgtcga gcagcgcctc
     2881 gcccagctcc gggtcgttcg cgaggaacac gaagtggcgc tcgatcgccg cgcggttgcg
     2941 gtggagcagc ccgagccagt cgtcgcggcc gaccacgagc ggcaggcgcg cgccgacgcg
     3001 atagcgcagc agcgcgccgg cccgcagcag cgcatcgacc gcctcgcgcg gcgcggtgac
     3061 cggggacggg agccggcacg tctcgccgac gtggcgcgag cgcagcgccg gctcctcctc
     3121 gtcgtcggtg ccgagcacga ccgggatgcc cgcgagcgcg agcggccgca cgaggttgat
     3181 gccgccgagc acgaacgccg gcgggcgagc gctgccggtc cgacggcggc ggagcgcggg
     3241 atcgggaatc ggaaccgctg cgtgcgtggc gccggtcggg tttcgggtca acagcatctc
     3301 gtcgagcgct cggatgatgc gcaatcgcga ttttcgccgg cccgggctgt cggcatcgtg
     3361 tccgggcccc gtggcgttct agccttttgg aaccagcggg attaccctgc gccgctcagc
     3421 gcacggttat tttggcctgt gcgagcgcgg cggcggcggc gacggcgaga tcgctgcgga
     3481 tctccatcca ctggtcgacg cgctcggtcc aggcgtgcag ctcgagcccg agccagtccg
     3541 ggcccatgcg caccacgatc gccttcggcg gcggggtctt cgcgatcggc tcgcactgcc
     3601 gcgccacctc ctggagcacg cggatcaccg acggcaggtc ggtcccgatc ggcacggcga
     3661 tcggcagctg gatcgagcgc tcgtggctcg agaacgtcca gttgaccacg cgctccgaga
     3721 tgagcttgcc gttcggcacg atgatctcgg agcccgtggt cgtgcgcacg gtgctcgcgc
     3781 ggatgccgat gtgctcgacg atgcccgagg agtcctcgat ctggatcacg tcgccgacct
     3841 tcaccggccg ctcgaagagc aggatcaggc ccgacacgaa gttgttgaag atgttctgca
     3901 gcccgaagcc cacgccgatc gagaacgcgc ccgcgaggat ggtgaacttc gtcatgtcga
     3961 agccgagcgc ggcgacgccc gcgaggaagc ccacgatcag gatcacatag ttgagcatgg
     4021 tcgagatcgc gtacggcatg ccgcggcgca tgttgaagcg cgtgtacacg tcctcctcga
     4081 gcaggaagcg cacgaagcgc gacagcagga acgccgccca gaccgacacg gcgaactcga
     4141 gcaggtcggc gagcgagacc cggatcgcgc cccagcggct ctcggcgatg aggatctcct
     4201 gcgcgctctt gaacaggcgg tcgcgcagtc cgagccggtc gagcgcgaac agcacgagca
     4261 ggcccgcggc cgcccactgc atgaagcgca gcacccgccg cgagatcagc gcccggtggc
     4321 gcttcacggc cccgagcagc gtaaacggcg gcaggtgcag cgccatcacg aacaggccgt
     4381 cggcgatctc gaccagcgcg tagagcatga cggcggcgta cgcggtcgcg agcagcgcgt
     4441 tgccgagcag gttcgccagc ccgacgtagc ccagcatgtt ggcggcgagc gacacgatcg
     4501 cgagcgcgag cgccgcccat cccgcccagt gcaggatcgc gcccgagcgc agcgcctggt
     4561 cgggcgagtc ccgcgcggcc gcgcgcgccg cgggccccga gcgcgcgagc agccaccccg
     4621 agaagacgac cgcgccgacc atctccgcga acacgatgag ccgcggcgcg agctgcagcg
     4681 gcgccgcgat caggcgcacc tggtcgacga cgtagaaggc gaccagcgcg tacagcaccg
     4741 ggtacatgcc gcgctcgatc agccgccgca ggatgatcac cgccggcgcg agcacgagcg
     4801 cgcccatcga ggcgtacagc agcacgggcg cgtccgggta gaggcggaag ccgccggcga
     4861 gcgtcaccag gagcgcggtc gccgccggga cgtcgaagat ggcgccggtg tcggcgaggc
     4921 gcggctcgtc cttgagccag cggcgcacgc gccggcgcgt ccagtacagg aacgcggtga
     4981 gcgcgacgaa ggcgagggcg tgcgccgcca tgcgttccga ctggcgctcg gcgtaggcct
     5041 cgaccgcgga cgcctgcgat gccagcgagc gccgcgcctc ttcggcgaag ctcggcccgg
     5101 cggaggccgc ggcccagagc ggcgggctgt cgcgctcgaa gatattctcc tcgagatcct
     5161 cgcgcgcgcg ccgcgccgcc gcgatcgcgt cggccatgcg cccgtcctgg gtcgcgacgc
     5221 ggttctgcag cgcgagcagg cgcgcgcgct gggcgccgat cgtggtgcgc gtgcgcgcga
     5281 tctcgcccgc gagcgcctcg agccgcttga cgatctcggg cggcgcctcg gtgcggctct
     5341 cctcgagcgt cttcgcccag gtcgccgcgg catcgtcgac ctgcgccgcc tcgcggtcga
     5401 ggcgctcgat gcgcgcggcg agccggcgcg cgagctcggc gaggttgcga cgcagcggcg
     5461 cccagcgcgc ctcgatgcgg cgcagcgtct cgatcgacga ggtgcgcgcg acggcccggc
     5521 ggttgtcgac caggcgcgcg tcgatctcgc gcgtgagcac gcgcaagtcg ctctcgacgc
     5581 cccgggcggt cgagtcgtcg cccgcctcct cgcgcaggtc gccgagcagg ttcgacgcgt
     5641 cctcggcgcg ccgcggcgcg tcggcgagcg ggaccgcttc gggcctgggc ggctcgggct
     5701 tcgcggcggc cttcgccttg tcgtcgggtt tcggcgcggg caccggcccg ccttgcgcga
     5761 gcgccgcgca gcagcccgcc gcgacggccg cggcgagcag gcatcgaagg gcgcgattca
     5821 gccagcgcga tttcacggtc gttcgtcttc ttgcgtcgct gggagcggga cagtgtgcgg
     5881 ccgcggcgtg ccggcagcgc cccccgcggc atggtccgaa cggactacgg gcgtcaaaac
     5941 tgccgggtga acgcgagccg cacgtagctg cggtcgaacg cgaacagcgg gatgttggac
     6001 acgccgacgt tgtagccgac gatgagcttg ggcgagaacc cgtgccagcg caggtcgcgg
     6061 cgcagcagcg tcgcctcgag cagcgagcgc cggtcgtggc gcgtcgcgcc gaagagcgga
     6121 tcgggcgcgt cgtagcggaa ccagtaggtc tcggcgcgca tgccgacggt ccacgcgctc
     6181 gcgagctcgg tctgcacttc gccgcgcagc ccttgcgcgt cgtacgccga cgacagcgcc
     6241 tgcgcctcct gcttgatgac cgagcttccg agccgcagca gcgtgctgcc gtggagcgcg
     6301 cgcgtccagt cgaggttgag cgcgtactgg ttcccggtga ggaagccgaa gtcctggtag
     6361 cgcagctcgc gtgcctcgag cgtagttgtg acccgcgtgt cgggccgcac gcgccagttg
     6421 tcgccgagct tggcgagggt gccgtcgtag agcgagcgct cgccgtagtt cgcgtagtgg
     6481 ccgcccgcct cgaagaagac gtcgtggctc gcgaacgcga aggtgtgccc gacggtgagc
     6541 gcgccgtaga acaggtcgaa gcggtgcccc ttgaagttgg tgtcctcggt gtagaggttg
     6601 acgtagttgt cgagcccggg gccgaagctc ttgcgcccgc cgagcgtcac catcagcccc
     6661 gttcccgcct tcgcgcgcgc gtcctcgttc agctggaacc gggcttcgcc gatcggcacg
     6721 tcggtcgcgc gcgtcgcgta gttgacgttc gagtcggaca cgatcgcgac atcgagcgtc
     6781 cagtagctct gccgctggcg gatgtcgcgc acgaagcgca gcgcgttcgc cttcgcctgc
     6841 ggcggcaggt cggtcgagag cgcgagctcg aagtggtaga gcgcggccgg gtagtcgcgc
     6901 tcgcggtaga gcgcgagcgc gaggtcgagg cgcacgcgca ccgcggtcgg atcgcggttc
     6961 aggatgcggc ggaactcctc gaccgcgccg gcgacgtcgc cgcgcgcgag cttcaggtgc
     7021 cccgagagga acagcacctg cagcgagggc tcgggcgcgg cgcgcagccg cgcgatcagg
     7081 cgctcggccg ggtcgagctt gccctgctcg agcagctcgg tggcgagctg cgtcgcctgc
     7141 tcgatcgtca ggttgagggt cggcggctcg ggatcctggg cgtgcgcgca gccgagcgcg
     7201 agcgcccagg cgaggaggag gaagcgcagc ggacgcgcca tgcgctacag catggcgcgc
     7261 ccgtgtgaca ggacgttcgt cctgagcttg tcgaaggact agcgcttcag cgccgctccg
     7321 cccaccaggg tgccggtctg ggacttcagg ttgaacgtca ttcccagctc ggccgcctgc
     7381 gggccgtaga agttgccgct cgcggtgccg gtcttgccgt ccgcggtggt catcgtgccc
     7441 gagagcgcgt tggtcgcggc cgggtaggtc agcgtgccgg tcatgttgta gccgggggcc
     7501 gcgctggacg cgccggtcgc caggtcggtg cgcaccgagc tcgaggtctg gactccgatc
     7561 gagcgggcgc ggaagtcggc gagcgccgag gcgttgcccg tcaccgagta ctggctcgtg
     7621 ccctcgaccg cgacgccgag catcgcgccg ttgtaggtcg cggtgccgtt ggtcgggatg
     7681 tcggtgccga gcgtgcgcgt gccgaccgag aacgcggccg cgttcgtcat ctgcccgctc
     7741 gcgatgtcgg tcgtgttcca gacgccgagg ctcgagaact gcagcttgag cgggttggtg
     7801 ctcgccgcgt cgggcgcgag gtaggtgacg gtgcggcgcg tggtggcgtc cgacttcgag
     7861 gaggtgtcga cgcgcaggcc gttcaccatc gtcgtcgtca ccgggccgtc gaacacgtcg
     7921 tcgatgccga gatcggggat cgcgacgtgg atcaccggcg tgttcgcgag cgacacctcg
     7981 ggcgccggca tgtcgttcaa cgagtcgaac gagccgaggc ccgcgggcgt gagcgcggtc
     8041 tgctgggtga aatcgatgtg gtgatcgacg gcggcgacct ggccgagcgg agcgagctgc
     8101 gggttgggtc cctgatc
//
LOCUS       DATDMW010000237        12978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1068861, whole genome shotgun sequence.
ACCESSION   DATDMW010000237 DATDMW010000000
VERSION     DATDMW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068861"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VI319_10020"
     CDS             <1..831
                     /locus_tag="VI319_10020"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY6864225.1"
                     /translation="PARKAQAAPPPGSVVQAVQAPAWIARDGGKIPAAPGMPLKQHDE
                     LHTGANARLWVGTPDGSVVKMGENGAVAIESALLGKDRTFHAALKVIQGAFRFTTEAL
                     AKFRGKRDVAVTIGTVTAGIRGTDLWGKSAADKQIVCLIEGKIEVTPPGEAAMTLDKS
                     LTFYIREKDQSQPVAPVPPEQLKVWAAETETEAGKGVARRGGKWRIVVSSKSAAAALK
                     TYEDIRAAGYAAELQPTKVNDKRVYTVQLTSFASKADADAAAKSLKDQGKLRDYKVAG
                     "
     gene            872..3043
                     /locus_tag="VI319_10025"
     CDS             872..3043
                     /locus_tag="VI319_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY6864226.1"
                     /translation="MSLLEHLNPEQLAAVTLPGQSALILAGAGSGKTRVLTTRIAWLI
                     QTGNASPGQVLAVTFTNKAAREMTTRLSAMLPINTRGMWIGTFHGLCNRFLRAHYRDA
                     GLPQAFQIMDSQDQLAAIKRAMKAMNADEDRFPPREVQYFINDAKEEGLRAGAVEAGD
                     ELAGRYAEIYAAYDEQCQREGSVDFAELLLRTFEVLSRNEILRAHYQRRFRHILVDEF
                     QDTNRLQYRWLKLLAAQGSAVFAVGDDDQSIYAFRGANVGNMAQFEHEFRVENLIRLE
                     QNYRSHGHILAAANALIAHNKKRLGKNLWTAAGEGEPVRIHEAQTDGYEAAWVVEEVQ
                     ALARSGLRLSDIAVLYRSNAQSRILEHALFSQGIPYRVYGGLRFFERMEIKHALAYLR
                     LVANPDDDAAFLRVVNFPARGIGARTLEQLQDTVKAAGGSLVQAAKATGGKPGAFVQM
                     IDALRAETQGLPLSETVDAMLARSGLVAHYQSEKDGADRVENLNELVNAAAAFTQEER
                     VVDVPDAEAPATAAAEPNDPLTAFLAHAALESGENQAAAGVDALQMMTVHSAKGLEFH
                     AVFITGLEEGLFPHEQSIMEDEGLEEERRLMYVAITRARTRLYLAFAQTRLLHGQTRY
                     NVASRFLDEIPKEAVRWLTPRFKPMGREPAAWAPAEERSAPVHKPDHGFRIGQAVMHP
                     KFGHGVIVSAEGSGTDARLQVNFGRQGMKWLALEYAKLTAA"
     gene            complement(3048..3296)
                     /locus_tag="VI319_10030"
     CDS             complement(3048..3296)
                     /locus_tag="VI319_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864227.1"
                     /translation="MAKRAKAKKQRPFWERGFQAHGYWLGKERLGRVALGPRGEWDGI
                     YRWQAGSREGQAKTLKEAKQAVEQAVLIGASQLQLFEQ"
     gene            complement(3317..3733)
                     /locus_tag="VI319_10035"
     CDS             complement(3317..3733)
                     /locus_tag="VI319_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY6864228.1"
                     /translation="MEAFFSAEASLASLLIATFLASTLIPLSSELVLLGVLKLHPDIA
                     WLALVVATTGNTLGGMTSYLIGRYFGSKKPLKHVERLRRWGAPALFFAWLPFVGDALC
                     IAAGWLKLDWRATTAYQAAGRFARYSAIAFAFGLTG"
     gene            3817..5346
                     /gene="ilvA"
                     /locus_tag="VI319_10040"
     CDS             3817..5346
                     /gene="ilvA"
                     /locus_tag="VI319_10040"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY6864229.1"
                     /translation="MRNDYLERILKARVYDVAVETPLELAPNLSARLGNRLLLKREDM
                     QPVFSFKCRGAYNKMTRLKPAALGRGVIAASAGNHAQGVALAARKLGCRATIVMPITT
                     PKIKVDAVRARGGEVVLEGDTYDEAYAEARRLERARRLAFVHPYDDPDVIAGQGTIGM
                     EILRQTARPIHAVFVPIGGGGLIAGIAAYVKRLAPEVKVYGVEPVDADAMARSLAAGR
                     VIALDHVGLFADGVAVKKVGAETFRLCRELVDGVILVDTDEICAAIKDVFEDTRSILE
                     PAGALAIAGAKAYVERSAVRGRKLVAIASGANTNFDRLRFVAERAESGEHREAILAVT
                     IPERPGSFKKFCATLGGRNITEFIYRIADAREAHVFVGIEVHGRAETAKIVKSLKRHE
                     LRTLDLSDDEMAKLHLRHMVGGHAPAGIGANGAPGELLYRFEFPERPGALMKFLSSMR
                     TDWNISLFHYRNQGADYGRVLVGMQVPRGEMRDLRNFLATLGYPYVDESRNPAYRLFL
                     G"
     gene            complement(5343..6020)
                     /locus_tag="VI319_10045"
     CDS             complement(5343..6020)
                     /locus_tag="VI319_10045"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY6864230.1"
                     /translation="MTPSEVYAWRKSERRRLLAAREAVAPEQQEEWRARIDAHLQRLF
                     GDSVHGVVAFCWPYRGEYDVRHFAAQLRRLGAATALPVVVAPGRPLAFRKWYPGVKMA
                     HGPLGIPYPEDSEALTPDHVLLPMLGWDAGGYRLGYGGGFFDRTLAALERRPRVIGLA
                     YELQYIPTIHPQPHDIPLDYVVTERGVHRREGDQLRFVDSGGLSSPVCYAGEVAPGYF
                     GEDPGKV"
     gene            6091..8082
                     /locus_tag="VI319_10050"
     CDS             6091..8082
                     /locus_tag="VI319_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY6864231.1"
                     /translation="MLFARFQGGTITPVRAVLAGLLLLAPIAAAARSSLVEDVAFFNA
                     RDAYQSHNKAKLGKAVEKLKGTPLETYGDFWQLRLRLDSASAEEVHEFLARNDKTVVG
                     DEMRKEWLRLLAKRERWDLFRQDYRAPSGDDPDPDVACYSLLGRWREGDDTAYTEFQA
                     QWNAAKELPAACVPLAESLMLSGRLTTRQVWERIRLLEERGMVKASKKLLAWLPRDQT
                     LEPARFSHILNAPMKYLEKPSVDVGTPLGRELAIVAISRLARNDPQAAATLWQEDLFA
                     RFPAEDHAYVWAMLATHGARNHLPESLDWFVKAGDMRLSDDQLAWRTRIALRELNWAE
                     VKGSIERMSPSARKDPAWIYWLGRAQAALGKADEAKALYESLADEYHFYGRLAAEELG
                     HAFEIPPQVAPPTPEEIDRVAAVPALQRALALYRMGLRTEGTQEWIYGVRGMDDRTLL
                     AAAELARRHQMWDRAINTADKTVADHDFTARYVVPFREVFAQHSTRWKLEEPWVLGVV
                     RQESRFIADAKSPAGANGLMQLMPSTARWVARQLGIKLSTSKVKEPTLNVTLGTYYLK
                     QVLKDLEGNPVLAAAAYNAGPKRARKWRDTKPLEGAIYAETIPLAETREYVKKVMLNT
                     VYYAAVLSGETRSLKERLGTIAPRPSSAGVMAVHDPSDV"
     gene            8192..9136
                     /locus_tag="VI319_10055"
     CDS             8192..9136
                     /locus_tag="VI319_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY6864232.1"
                     /translation="MARILVLGGSGFIGRHLVEALVEQGHTVVVPSRRRERSKHLITL
                     PTVDVVEADVHDEETLARLLAGSEAAINLVGILHSRPGSPYGPDFARVHVELPRKLID
                     ACRRTRVRRVLHLSALNASRDGPSEYLRSKGEGEALVLAAQDGVGVTAFRPSVVFGPE
                     DRFLNEFARWQRWLPFIMVPCPQARLQPVYVGDVVRCLVLALDERASFGKRYDLCGPN
                     AYTMRELWRIAGRLSGHPRPLIGLGDKLSYLQAWMLEWAPGKPMSRDNFRSLQVDAVS
                     AAPLPFGVKPTALEAGAAYLAGAGPEARYSNYRYTAGR"
     gene            9174..10316
                     /locus_tag="VI319_10060"
     CDS             9174..10316
                     /locus_tag="VI319_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HEY6864233.1"
                     /translation="MKTYAVGGAVRDELLGLPVSDRDYVVVGATAEQMIERGFKPVGK
                     DFPVFLHPETHEEHALARTERKVGRGYKGFTVYAAPDVTLEQDLERRDLTINAMARDE
                     HGALVDPFGGARDLERRVLRHVSPAFVEDPVRILRVARFAARFGFSVAPETLALMRRM
                     VENGEADALVPERVWQEFSKGLMERGARAMFGVLARCGLLARLLPEVALAYQDGEPAN
                     DASRILERGLERAAAGDASLAERYALVLADARDADAAGAVSERLRAPTECRDLAVLWH
                     RQGRAASEAPAAEAAALLAMLEGTDAFRRPERLASLIRVCEYRRFGERGWAESPYPPR
                     VALQVALAAASGVDAAEIARRAAGRDIGERLRRARVDAIRSVNDRR"
     gene            10334..10771
                     /locus_tag="VI319_10065"
     CDS             10334..10771
                     /locus_tag="VI319_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY6864234.1"
                     /translation="MPFPASVLRCALLVAVCGSAAAADGALSRAELERRSPRLARHFD
                     AIDADGDGRVTAAEIRAWRRSDRSRGDREAGRRAGFDHLFRRADADGDGALSRDEARR
                     GLPRLARKFERVDADGDGRITIEEAHAWLDARRGAVRRAASAK"
     gene            complement(10746..10940)
                     /locus_tag="VI319_10070"
     CDS             complement(10746..10940)
                     /locus_tag="VI319_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEY6864235.1"
                     /translation="MLKWALTLAVALIVLTAVSPWLAKRGLGRLPGDVVVNYRGRRYY
                     LPFTSTILLSLALTLLTRLV"
     gene            10997..11614
                     /locus_tag="VI319_10075"
     CDS             10997..11614
                     /locus_tag="VI319_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6864236.1"
                     /translation="MIELYTWGTPNGRKVSVMLEECGLPYEVHKVDIGAGEQFRPDYL
                     RINPNGKIPSIVDPDGPGGAPIAMMESGAILVYLAAKTGRLLPKDDRGRYKALEWLMF
                     QMGSVGPMFGQVHHFLRAAKEQVPYAVQRYTKEKDRLYGVLDRRLAETDYLAGEYSIA
                     DVATYPWVARYEWHKTDLGAHPNVKRWFDAIGARAAVKRGMAVPA"
     gene            complement(11622..12284)
                     /locus_tag="VI319_10080"
     CDS             complement(11622..12284)
                     /locus_tag="VI319_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864237.1"
                     /translation="MRAVRAVAAALLLVALSAAAQDPRQLLEQARKRWQDSPHGPMLE
                     RILPPTFEPRQLPEPGSPGADLVLRYCVQCHNLPNPAMHERAKWPAVFERMVLRMRGR
                     GNMGALMKDMMAGVSAPSVEEAATLLAYLQKHAQRPLDPIRTPVNTPEAQSFRLACQQ
                     CHVLPDPRRHTASEWPAVVARMQRNMEWMNRVVGNRHPPDEPQLRVEEIDAYLKRYAR
                     RN"
     gene            complement(12286..>12978)
                     /locus_tag="VI319_10085"
     CDS             complement(12286..>12978)
                     /locus_tag="VI319_10085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY6864238.1"
                     /translation="ASLPPGFAGVVLGNEVLDAMPADVIRVRAGAIEQAGVADDRGRL
                     GWSWRPAPGALAKDALALHLPEGYRTELQRAACAFVRSLADVLERGIALFVDYGFPAR
                     EYYHAQRSDGTLMCHYRHHAHEDPFFLPGLQDITTHVDFSAVAHAAAEAGLDVCGYTS
                     QAQFLINCGITEALAQTPSEKAASYLPLASQVNRLTSPAEMGELFKVIALCRGHSAPL
                     LGFASGDRRHTL"
BASE COUNT         2020 a         4520 c         4629 g         1809 t
ORIGIN      
        1 cccgcgcgca aggcgcaggc ggcgccgccg cccggctcgg tggtgcaggc ggtccaggcg
       61 ccagcgtgga tcgcgcgcga cggcggcaag atccccgcgg cgcccggcat gccgctcaag
      121 cagcacgacg agctgcacac cggcgcgaac gcgcgcctgt gggtcggcac gcccgacggc
      181 agcgtggtga agatgggcga gaacggcgcg gtggcgatcg aaagcgcgct gctcggcaag
      241 gaccgcacct tccacgcggc gctcaaggtg atccagggcg cgttccggtt cacgaccgag
      301 gcgctcgcga agttccgcgg caagcgcgac gtggccgtca cgatcggcac ggtcaccgcg
      361 ggcatccgcg gcaccgacct ctgggggaag tcggccgccg acaagcagat cgtttgcctg
      421 atcgagggca agatcgaagt cacgccgccg ggcgaggcgg cgatgaccct ggacaaatcg
      481 ttgacgttct acatccgcga gaaggaccag tcgcagccgg tcgccccggt tccgcccgag
      541 cagctgaagg tctgggcggc ggagaccgag accgaggcgg gcaagggcgt cgcgcggcgc
      601 ggcggaaaat ggcggatcgt cgtgtcgtcg aaatcggcgg ctgcggcgct gaagacctac
      661 gaggacatcc gcgcggccgg ctacgccgcc gagctgcagc cgaccaaggt gaacgacaag
      721 cgcgtctaca ccgtgcagct cacgagcttc gcgagcaaag ccgatgccga cgcggccgcg
      781 aagagcctga aggaccaggg gaagctgcgc gactataaag tcgccggctg atctccgcgt
      841 tttgtaaaat cggggctttg ccgcagcccc gatgtcgctc ctcgaacacc tcaaccccga
      901 gcaactcgcc gccgtcacgc tccccggcca gtccgcgctg atcctcgcgg gcgcgggaag
      961 cggcaagacg cgcgtgctca cgacgcgcat cgcctggctg atccagaccg gcaacgcgag
     1021 cccgggccag gtcctcgccg tgaccttcac gaacaaggcg gcgcgcgaga tgacgacgcg
     1081 cctctcggcg atgctgccga tcaacacgcg cggcatgtgg atcggcacct tccacggcct
     1141 gtgcaaccgc ttcctgcgcg cgcactaccg cgatgccggg ctgccgcagg cgttccagat
     1201 catggactcg caggaccagc tggcggcgat caagcgcgcc atgaaggcga tgaacgccga
     1261 cgaggaccgc ttccccccgc gcgaggtgca gtacttcatc aacgacgcga aagaggaagg
     1321 cctgcgcgcg ggcgccgtcg aggcgggcga cgagctcgcc ggccgctacg ccgagatcta
     1381 cgccgcctac gacgagcagt gccagcgcga aggctcggtc gatttcgccg agctgctgct
     1441 gcgcacgttc gaggtgctgt cgcgcaacga gatccttcgg gcgcactacc agcgccgctt
     1501 ccgccacatc ctggtcgacg agttccagga cacgaaccgg ctgcagtacc gctggctgaa
     1561 gctgctcgcg gcgcagggga gcgcggtgtt cgccgtcggc gacgacgacc agtcgatcta
     1621 cgccttccgc ggcgcgaacg tcggcaacat ggcgcagttc gagcacgagt tccgggtcga
     1681 gaatctcata cgcctcgagc agaactaccg ctcgcacggg cacatcctgg ccgcggcgaa
     1741 cgcgctcatc gcgcacaaca agaagcgcct gggcaagaac ctgtggaccg ccgcgggcga
     1801 gggcgagccg gtgcgcatcc acgaggcgca gaccgacggc tacgaggcgg cctgggtggt
     1861 cgaggaggtg caggcgctcg cgcgctcggg cctgcggctc tccgacatcg ccgtgctcta
     1921 ccgctccaac gcgcagtcgc ggatcctcga gcacgcgctg ttctcgcaag ggatcccgta
     1981 ccgcgtctac ggggggctgc gcttcttcga gcgcatggag atcaagcacg cgctcgcgta
     2041 cctgcgcctg gtcgcgaatc ccgacgacga cgcggccttc ctgcgcgtgg tgaacttccc
     2101 cgcgcgcggc atcggcgcac gaacgctcga gcagctgcag gacaccgtca aggccgccgg
     2161 cggcagcctg gtccaggcgg cgaaggcgac cggcggcaag cccggcgcct tcgtgcagat
     2221 gatcgacgcg ctgcgcgccg agacgcaggg gctgccgctg tccgagacgg tcgacgccat
     2281 gctcgcgcgc agcggcctcg tcgcgcacta ccagagcgag aaggacggcg ccgaccgcgt
     2341 cgagaacctg aacgagctgg tgaacgccgc ggccgcgttt acccaggagg agcgcgtcgt
     2401 cgacgtgccc gacgcagagg cgcccgcgac cgcggcggcc gagccgaacg acccgctcac
     2461 cgccttcctc gcccacgccg cgctcgagtc gggcgagaac caggcggccg cgggcgtcga
     2521 cgcgctgcag atgatgacgg tgcactcggc caagggcctc gagttccacg ccgtgttcat
     2581 caccggtctc gaggaggggc tgttcccgca cgagcagagc atcatggagg acgagggcct
     2641 ggaggaggag cggcgcctca tgtatgtggc gatcacgcgg gcgcgcacgc ggctctacct
     2701 cgccttcgcg cagacgcgcc tgctgcacgg gcagacgcgc tacaacgtcg cctcgcgctt
     2761 tctcgacgag atcccgaagg aggcggtacg ctggctgacg ccgcggttca agccgatggg
     2821 ccgcgagccc gcggcgtggg cgccggccga ggagcggtcc gctccggtgc acaagcccga
     2881 ccacggcttc cgcatcggcc aggccgtgat gcatcccaag ttcgggcacg gcgtgatcgt
     2941 cagcgcggaa ggctcgggca ccgacgcgcg gctgcaggtg aacttcgggc gacagggcat
     3001 gaaatggctc gcgctcgaat acgcgaagct cacggcggcg tagccggcta ctgctcgaac
     3061 agctgcagct gactcgcgcc gatcagcacg gcctgctcga ccgcctgctt cgcttccttc
     3121 agggtttttg cctggccttc gcgactgccc gcctgccagc ggtagatgcc gtcccattcg
     3181 ccgcggggcc cgagcgcgac gcggccgagc cgctccttgc ccagccagta gccgtgcgcc
     3241 tggaagccgc gctcccagaa cggccgctgc ttcttcgcct tcgcgcgctt ggccatgacg
     3301 acctcccggc ggaattctaa ccggtcaggc cgaaggcgaa cgcgatcgcg gagtagcgcg
     3361 cgaagcgtcc cgccgcctgg taagccgtcg tcgcgcgcca gtcgagcttg agccagccgg
     3421 cggcgatgca cagggcatcg cccacgaacg gcagccacgc gaagaacagc gccggcgcgc
     3481 cccagcggcg caggcgctcg acgtgcttga gcggcttctt ggacccgaag tagcggccga
     3541 tcaggtacga ggtcatgccg cccagcgtat tgccagtggt cgcgacgacg agcgcgagcc
     3601 aggcgatgtc cgggtgcagc ttgagcacgc cgaggagcac cagctccgac gacagcggga
     3661 tcagcgtcga ggcgagaaac gtcgcgatca gcagcgacgc caggctcgct tcggcggaga
     3721 agaaggcttc cacggcgcac agaatagaga gaaaaccggg actcgtgtac agtatttggg
     3781 accccccctc ggagggggtc tctttttttt gaaaaaatgc gcaacgacta cctcgaacgc
     3841 atcctgaaag cccgcgtcta cgacgtcgcg gtcgagaccc cgctcgagct cgcgcccaac
     3901 ctctcggcgc ggctcggcaa ccggctgcta ctgaagcgcg aggacatgca gcccgtgttc
     3961 tccttcaaat gccgcggggc gtacaacaag atgacgcggc tcaagcccgc ggccctcggg
     4021 cgcggcgtga tcgccgcctc cgcggggaat catgcgcagg gcgtggcgct cgcggcgcgc
     4081 aagctcgggt gccgcgcgac catcgtgatg ccgatcacga cgcccaagat caaggtcgat
     4141 gccgtccggg cgcgcggcgg cgaggtcgtg ctcgagggcg acacctacga cgaagcctac
     4201 gccgaggccc ggcgcctgga gcgcgcgcgc aggctcgcct tcgtccatcc ctacgacgac
     4261 ccggatgtca tcgcgggcca gggcacgatc ggcatggaga tcctgcggca gaccgcgcgg
     4321 ccgatccacg cggttttcgt cccgatcggc ggcggcgggt tgatcgccgg catcgcggcg
     4381 tacgtgaagc ggctcgcgcc ggaggtgaag gtgtacggcg tcgagccggt cgacgccgat
     4441 gcgatggcac gctcgctggc ggcgggccgc gtgatcgcgc tcgaccacgt cggcctgttc
     4501 gccgacggcg tcgcggtgaa gaaggtcggg gcggagacct tccgcctgtg tcgcgagctc
     4561 gtcgacgggg tgatcctcgt cgacaccgac gagatctgcg cggcgatcaa ggacgtgttc
     4621 gaggacacgc gctcgatcct cgagcccgcc ggcgctctcg cgattgccgg cgccaaggct
     4681 tacgtcgagc gctcggccgt gcgcggcagg aagctggtcg cgatcgcctc gggcgccaac
     4741 accaacttcg accggctgcg cttcgtcgcg gagagggctg agtcgggcga gcaccgggaa
     4801 gcgatcctcg cggtcaccat cccggagcga cccggcagct tcaagaagtt ctgcgcgacg
     4861 ctgggcgggc gcaacatcac cgagttcatc taccgcatcg ccgacgcgcg cgaggcgcac
     4921 gtgttcgtcg gcatcgaggt ccacgggcgc gccgagaccg cgaagatcgt gaagagcctg
     4981 aagcgccacg agctcaggac gctcgacctc tcggacgacg agatggcgaa gctgcacctg
     5041 cgtcacatgg tcggcggcca cgcgcccgcg gggatcggcg cgaacggcgc gcccggggag
     5101 ctgctctacc gcttcgagtt cccggaacgg cccggcgcgc tgatgaagtt cctctcgagc
     5161 atgcgcaccg actggaacat cagcctgttc cactaccgca accagggcgc cgactacggt
     5221 cgcgtgctgg tcgggatgca ggtgccgcgc ggcgagatgc gcgacctccg gaatttcctc
     5281 gcgaccctcg gctacccgta cgtcgacgag agccgcaacc cggcgtatcg gctgttcctc
     5341 ggctagacct tccccgggtc ttcgccgaaa tacccgggcg cgacttcgcc cgcgtagcag
     5401 accggcgacg agagtccgcc cgagtcgacg aagcgcagct gatcgccctc gcggcggtgc
     5461 acgccgcgct cggtgacgac gtagtcgagc gggatgtcgt gcggctgggg gtggatcgtc
     5521 gggatgtatt gcagctcgta cgcgagcccg atcacccgcg ggcgccgctc gagggcggcc
     5581 agggtgcgat cgaagaagcc gccgccgtac ccgagccgat agccgccggc atcccagccg
     5641 agcatcggca ggagcacatg gtccggagtg agcgcttccg aatcctccgg ataggggatg
     5701 ccaagtggac catgcgccat cttcaccccc ggataccatt tgcggaaggc gagcggccgg
     5761 ccgggcgcga cgaccacggg cagcgcggtg gccgcgccga gccgccgcag ctgggccgcg
     5821 aagtggcgca cgtcgtattc gccgcggtag ggccagcaga aagcgacgac gccgtgcacc
     5881 gagtcgccga acaaacgttg tagatgagca tcgatgcgcg cgcgccactc ttcctgctgc
     5941 tcgggggcga cagcctcccg ggcggcgagc aggcggcggc gctcactttt gcgccaggcg
     6001 taaacctcgg acggagtcat gttgtgatgc aagctcaaga aatgcgcgga agccgttcag
     6061 tttccggcgg aaacgcgtga tggctgtgct atgcttttcg ctcgatttca gggaggcact
     6121 ataacaccgg tcagagccgt cctcgcgggg ctgctgctgc tcgccccgat cgccgccgcg
     6181 gccaggtcgt cgttggtgga ggacgtcgct ttcttcaacg cccgcgacgc ctaccagtcg
     6241 cacaacaagg cgaagctcgg caaggcggtc gagaagctga agggcacccc gctcgagacc
     6301 tacggcgact tctggcagct tcggctgcgc ctggacagcg cctcggccga ggaggtccac
     6361 gagttcctcg cgcgcaacga caagaccgtc gtcggcgacg agatgcgcaa ggaatggctg
     6421 cggctgctcg cgaagcgcga gcgctgggac ctgttccgcc aggattatcg cgcgccctcg
     6481 ggggacgatc ccgaccccga tgtcgcctgc tactcgctgc tcggccgctg gcgcgagggc
     6541 gacgacaccg cgtacaccga gttccaggcg cagtggaacg ccgcgaagga gctgcccgcc
     6601 gcgtgcgtgc cgctcgccga gagcctgatg ctctcggggc gcctcacgac gcgccaggtc
     6661 tgggagcgca tccggctgct cgaagagcgc ggcatggtga aggcctcgaa gaagctgctc
     6721 gcgtggctgc cgcgcgacca gacgctcgag ccggcgcgct tctcgcacat tttgaacgcg
     6781 ccgatgaagt atctcgagaa gccgagcgtg gatgtcggca cgccgctcgg gcgcgagctc
     6841 gcgatcgtcg cgatctcgcg cctcgcccgg aacgacccgc aggcggccgc gaccttgtgg
     6901 caggaggacc tgttcgcgcg cttcccggcc gaagaccacg cgtacgtgtg ggcgatgctc
     6961 gccacccacg gggcgcgcaa ccacctgccc gagagcctcg actggttcgt gaaagcgggc
     7021 gacatgcggc tctcggacga ccagctcgcg tggcgcacgc gcattgccct gcgcgagctg
     7081 aactgggccg aggtcaaggg atcgatcgag cgcatgtcgc cgagcgcgcg caaggatccg
     7141 gcgtggatct actggctcgg gcgcgcgcag gcggccctcg gcaaggccga cgaggccaag
     7201 gcgctctacg agagcctggc cgacgaatac catttctacg gccggctcgc cgccgaggag
     7261 ctcgggcacg cgttcgagat cccgccgcag gtcgcgccgc cgacgcccga ggagatcgat
     7321 cgggtggccg cggtgcccgc gctgcagcgc gcgctcgcgc tttaccggat gggcctgcgc
     7381 accgaaggca cgcaggaatg gatctacggc gtgcgcggca tggacgaccg caccctgctc
     7441 gccgcggcgg agctcgcgcg ccgccaccag atgtgggacc gcgccatcaa caccgcggac
     7501 aagaccgtcg ccgaccacga cttcaccgcg cgctacgtcg tgcccttccg cgaggtcttc
     7561 gcgcagcact cgacgcgctg gaagctcgag gagccgtggg tgctgggcgt ggtgcgccag
     7621 gagagccgct tcatcgccga cgccaagtcg cccgccggcg cgaacggcct gatgcagctc
     7681 atgccctcga ccgcgcgctg ggtcgcgcgc cagctcggga tcaagctctc gacctcgaag
     7741 gtcaaggagc ccaccctcaa cgtgactctc gggacctact acctgaagca ggtcctgaag
     7801 gatctcgagg gcaacccggt gctcgccgcg gccgcttaca acgccggtcc gaaacgcgcg
     7861 cgcaagtggc gcgacaccaa gccgctcgag ggcgcgatct acgccgagac catcccgctc
     7921 gcggagacgc gcgagtacgt gaagaaggtg atgctcaata ccgtctatta cgccgcggtg
     7981 ctctcggggg agacgcgctc gctgaaggag cgcctcggca ccatcgcgcc gcggccgtcg
     8041 agcgcgggcg tgatggcggt gcacgacccc tcggacgtct gacgagcgcc gtctgaccgg
     8101 cgcgtgacgt ccggcgattg cgccgcgcgt cccgcgccgc ggccggttaa actacgccgg
     8161 tcggcttcct cctcatccct catccgaaat cgtggcccga attctcgtcc tcggcggcag
     8221 cggattcatc ggccgacact tggtcgaagc gctggtcgag cagggccata cggtcgtcgt
     8281 gccgagtcgc cggcgcgagc gctccaagca cctcatcacg ctgcccacgg tggacgtggt
     8341 cgaggccgac gtccacgacg aggagacgct cgcgcggctt ctggcgggca gcgaggcggc
     8401 gatcaacctc gtcggcatcc tgcacagccg gccgggctcg ccgtatgggc ccgattttgc
     8461 gcgggtgcac gtcgagctgc cgcgcaagct cattgacgcc tgccggcgca cccgcgtgcg
     8521 gcgcgtgctg cacctcagcg cgctgaacgc gagccgcgac ggcccgagcg agtacctgcg
     8581 ctccaaaggc gagggcgagg cgctggtgct cgcggcgcag gatggcgtcg gcgtgaccgc
     8641 gttccggccc tcggtcgtat tcggacccga ggaccgcttc ctgaacgagt tcgcgcgctg
     8701 gcagcgctgg ttgccgttca tcatggtgcc ctgcccgcag gcgcgcctgc agccggtgta
     8761 cgtgggcgat gtcgtgcgct gcctcgtgct cgcgctcgac gagcgcgcga gcttcggcaa
     8821 gcgctacgat ctgtgcggcc cgaacgcgta cacgatgcgc gagctctggc gcatcgccgg
     8881 ccgcctctcg gggcacccgc ggccgctcat cggcctcggc gacaagcttt cctacctgca
     8941 ggcgtggatg ctcgaatggg cgcccggaaa gccgatgtcg cgcgacaatt tccgctccct
     9001 gcaggtggac gcggtgagcg ccgcgccgct cccgttcggc gtgaagccga ccgctctcga
     9061 agcgggcgcg gcctacctcg cgggcgccgg ccccgaggcg cgctacagca actaccggta
     9121 taccgcgggt cgctgaagga ccgcgtgcct cgctcagccg cccgcccctc gccgtgaaaa
     9181 cgtacgcggt cggcggcgcg gtgcgcgacg agctgctcgg cctgccggtc tccgaccgcg
     9241 actacgtggt ggtcggcgcg accgccgagc aaatgatcga gcgcggattc aagcccgtcg
     9301 gcaaggactt cccggtgttc ctgcatcccg agacgcacga ggagcacgcg ctcgcgcgca
     9361 ccgagcgcaa ggtcggccgc ggctacaagg gtttcaccgt ctacgccgcg cccgacgtca
     9421 cgctcgagca ggacctcgag cgtcgcgatc tgaccatcaa cgccatggcg cgcgatgagc
     9481 acggggcgct ggtcgatccc ttcggcggcg cgcgcgatct cgagcgccgc gtgctgcgcc
     9541 atgtgagtcc ggcgttcgtc gaggatccgg tgcgcatcct gcgcgtggcg cgcttcgccg
     9601 cgcgcttcgg attctcggtg gcgcccgaga cgctcgcgct catgcgccgg atggtggaga
     9661 acggcgaggc cgacgcgctc gtcccagagc gcgtgtggca ggaattctcc aagggactga
     9721 tggagcgcgg cgcgcgggcg atgttcggcg tgctcgcgcg ctgcggcctg ctcgcgaggc
     9781 tcctgcccga ggtcgccctc gcgtaccagg acggcgagcc ggcgaacgac gcgtcgcgga
     9841 tcctcgagcg cggcctcgag cgggcggcgg cgggcgacgc gtcgctcgcc gagcgctacg
     9901 ccctggtcct cgccgacgcg cgcgatgccg acgccgctgg ggcggtgtcc gagcgcttgc
     9961 gcgcgccgac cgagtgccgc gacctcgcgg tgctgtggca ccgccaaggg cgcgcggcga
    10021 gcgaggcgcc cgcggccgag gcggcggcgc tgctcgcgat gctggagggg accgacgcct
    10081 tccgccggcc cgagcggctg gcgtcgctga tccgcgtgtg cgagtaccgg cgcttcggcg
    10141 agcgcggttg ggccgagtcg ccgtacccgc cgcgcgtcgc gttgcaggtc gcgctcgcgg
    10201 ccgcgtccgg cgtcgacgcg gccgagatcg cccggcgcgc cgcgggccgc gacatcggcg
    10261 agcgcctgcg ccgggcgcgc gtcgacgcga tccggtcggt caacgacagg cgatgaatcc
    10321 ccgttgctcg gggatgccat tccccgcatc ggtcctgcgc tgcgcgctgc tcgtcgcggt
    10381 gtgcggctcc gccgccgccg ccgacggcgc gctgtcgcgg gccgagctcg agcggcgctc
    10441 gccgcgcctc gcgcgccact tcgatgcgat cgacgcggac ggcgacgggc gcgtcacggc
    10501 ggccgagatc cgcgcttggc gccgatcgga ccgctcgcgc ggcgatcgcg aggccgggcg
    10561 ccgtgccggc ttcgaccatc tgttccgccg cgccgacgcg gacggcgacg gcgcgctgtc
    10621 gcgcgacgag gcgcgtcgcg gcttgccgcg cctggcgcgg aaattcgagc gcgtcgacgc
    10681 cgacggcgac gggcgcatca cgatcgagga ggcgcacgcc tggctcgacg cgcgccgcgg
    10741 cgccgtcaga cgagccgcgt cagcaaagtg agcgcgagcg acagcaggat cgtgctggtg
    10801 aacggcaggt agtagcgccg gccgcggtag ttgacgacga cgtcgcccgg aaggcgtccg
    10861 aggccgcgct tcgcgagcca gggtgaaacc gcggtgagca cgatcagcgc gacagccagg
    10921 gtcagcgccc atttcagcat ggcgctaaac tcgcgcgcat ttcgcgcacg ttcgaatgga
    10981 agcgaggcgg tcggagatga tcgagctcta tacttgggga acgcccaacg gccggaaggt
    11041 ctcggtcatg ctggaggagt gcgggctgcc gtacgaggtg cataaggtcg atatcggcgc
    11101 gggcgagcag ttccgtcccg actatcttcg catcaatccc aacggcaaga taccctcgat
    11161 cgtcgatccc gacggccccg gcggcgcgcc catcgcgatg atggagtccg gcgcgatcct
    11221 cgtctacctc gcggccaaga ccggccgcct gctgccgaag gacgatcgcg gccgctacaa
    11281 ggcgctcgag tggctcatgt tccagatggg ctcggtgggg ccgatgttcg gccaggtgca
    11341 tcacttcctg cgcgcggcga aggagcaggt gccttacgcg gtccagcgct acacgaaaga
    11401 aaaggaccgg ctgtacggcg tgctcgaccg tcggctcgcg gagaccgact acctggcggg
    11461 cgaatactcg atcgcggacg tggcgaccta tccctgggtg gcgcggtacg agtggcacaa
    11521 gaccgatctc ggcgcgcacc cgaacgtgaa gcgctggttc gacgcgatcg gcgcgcgggc
    11581 cgcggtgaag cgcggcatgg cggttccggc ctgaccggat ctcagttgcg gcgcgcatag
    11641 cgcttcaggt acgcgtcgat ctcctcgacg cgcagctgcg gctcgtcggg cggatggcgg
    11701 ttgccgacga cgcggttcat ccattccatg ttccgctgca tgcgcgcgac caccgcgggc
    11761 cactccgacg ccgtgtgccg gcgcggatcg ggcagcacgt ggcactgctg gcacgcgagc
    11821 ctgaacgact gcgcctccgg cgtgttcacc ggcgtgcgga tcgggtcgag gggacgctgc
    11881 gcgtgcttct gcaggtacgc gagcagcgtc gcggcctctt ccacgctcgg cgccgacacg
    11941 ccggccatca tgtccttcat gagcgctccc atgttcccgc ggccgcgcat gcgcagcacc
    12001 atgcgctcga acacggccgg ccatttcgcg cgctcgtgca tcgccgggtt cggcaggttg
    12061 tggcactgca cgcagtagcg caggacgagg tctgcgcccg gcgagccggg ctccgggagc
    12121 tggcgcggct cgaaggtcgg cggcaggatg cgctcgagca tcgggccgtg cgggctgtcc
    12181 tgccagcgct tgcgcgcctg ctcgagaagc tgccgcggat cctgcgccgc ggcggacagc
    12241 gcgacgagga gcagcgctgc cgcgacggct cggacggccc gcatgctaga gcgtgtgccg
    12301 gcggtcgccg gaggcgaacc cgagcagcgg cgccgagtgc ccgcggcaaa gcgcgatgac
    12361 cttgaacagc tcgcccatct cggccgggct cgtcaggcgg ttcacctgcg aggcgagcgg
    12421 caggtacgag gccgccttct ccgacggcgt ctgcgccagg gcctcggtaa tgccgcagtt
    12481 gatcaggaac tgcgcctggc tcgtgtagcc gcagacgtcg agaccggcct cggcggcggc
    12541 gtgcgcgacc gcgctgaaat cgacatgcgt ggtgatgtcc tgcagccccg ggagaaagaa
    12601 cggatcctcg tgggcgtgat gacggtagtg gcacatcagc gtgccgtcgc tgcgctgcgc
    12661 gtggtagtac tcgcgcgcgg gaaagccgta gtcgacgaag agcgcgatgc cgcgctcgag
    12721 cacgtcggcg agcgagcgca cgaacgcgca cgcggcgcgc tgcagctcgg tgcggtagcc
    12781 ctcgggcagg tgcagcgcga gtgcgtcctt cgcgagcgcc ccgggtgccg ggcgccagga
    12841 ccacccgagg cgcccgcggt cgtccgcgac gccggcctgc tcgatcgcgc ccgcgcgcac
    12901 gcggatcacg tccgcgggca tggcgtcgag cacttcgttg ccgaggacga cgcccgcgaa
    12961 ccccggcggc aggctcgc
//
LOCUS       DATDMW010000238         7685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1442542, whole genome shotgun sequence.
ACCESSION   DATDMW010000238 DATDMW010000000
VERSION     DATDMW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442542"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VI319_10090"
     CDS             complement(<1..659)
                     /locus_tag="VI319_10090"
                     /inference="COORDINATES: protein motif:HMM:NF027838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY6864239.1"
                     /translation="MTLLPRSLLWRTFLLLALLVVVATGAWFQIFRAYEAAPRARVIA
                     QNLVSTINLTRAALVNAQPERRRQLLEDLSEREGLEIYPSEPGERIVPPPDREMVRLV
                     AEAVRERLGAQTRFASRREGRPGFWVSFHIDEDEYWVRVPRERIERAIALQWLYWGAL
                     ALVLSLVAAYFIVSRVSRPLKRLAEAATAIGHGKKPEPVPETGPSEIRTLSRSFNDMS
                     R"
     gene            complement(679..1419)
                     /gene="ompR"
                     /locus_tag="VI319_10095"
     CDS             complement(679..1419)
                     /gene="ompR"
                     /locus_tag="VI319_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEY6864240.1"
                     /translation="MAEPKTRVLVVDDDLRLRDLLQRYLTEQGFTVHTVSDAAGMDKI
                     MSRERFDLMVLDLMLPGEDGLSICRRLRGTKNMVPIIMLTAKGEEVDRIVGLEMGADD
                     YRPKPFNPRELVARIHAVLRRRAAPVPPGAPSSEPEKVRFGSMTVDLATRTLEKDGKT
                     VSLTTGEFALLKVLVTHPRTPLSRDKLMELARGREYEVFDRSIDVQVSRLRKLVERDP
                     ARPAYIQTVWGFGYVFVPDGEPSAQASS"
     gene            1565..1867
                     /locus_tag="VI319_10100"
     CDS             1565..1867
                     /locus_tag="VI319_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864241.1"
                     /translation="MKPSRSFLLLAALAALACSGPALAQHQGRGGRGGQPQKQQQAQQ
                     RPAPPPAQGPQQHEATYRQQPQRQNGRMSDEERQQLRRDISDHGRDIYQDRGGARR"
     gene            1957..2451
                     /locus_tag="VI319_10105"
     CDS             1957..2451
                     /locus_tag="VI319_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864242.1"
                     /translation="MPSRRTLQWSALPVAAWLGLAASGAWAADTDAGERKNAFTFETG
                     ADELLKRSQDLRMVGPRNMFYLATLKKDFGDPRGVRPFVAGGVGLIAPRLTGSMSRNP
                     GGSAAQAAAGLRYESGRTSVIAEYRYLWAPSSDLDARGEMGDAQGKLNLGGQAYFLGL
                     GIKF"
     gene            complement(2461..3426)
                     /locus_tag="VI319_10110"
     CDS             complement(2461..3426)
                     /locus_tag="VI319_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864243.1"
                     /translation="MFRAVAFWLGALLASGAFAQSQYPGRPLHLVVGFAPGGASDIIS
                     RAMSDPLQRSFGQPVIVDNKPGAGSSLAAELVAKSAPDGYTVMIASPSAVFVNPIIMK
                     NINYVTERDFVPVTLVTTSPLVVAVHPSVPASTLRELIDYAKKNPGKLNFATSGNGSA
                     PHLGTVLLASLTGIHMVHVPYKSGGLAVQSVLAGDTQLTIATPPSVLPQVKAGRLRGL
                     AMTSAERSPLVPELPGMKEAGVDGYNYTFWYGMFAPAGTPRAVINRLFDATVTALRDP
                     KLKQILATDGTETAPSKSPEEFGAWLREEGKLWARLVKDSGAKVD"
     gene            complement(3427..4173)
                     /locus_tag="VI319_10115"
     CDS             complement(3427..4173)
                     /locus_tag="VI319_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6864244.1"
                     /translation="MDKVVLITGAGRGIGAATATLAARRGWAVCVNYLKNRDAAEAVV
                     RDIEKAGGRAVAIAADVSSETDVVRLFKECDRALGPVSALVNNTGILERQIRVEDIDA
                     ARLGRILTTNVVGSFICAREAVRRMSTKRGGKGGAIVNVSSAASRIGSPGEYVDYAAS
                     KGAIDSFTLGLAKEVAAEGIRVNAVRPGVIYTEIHASGGEPGRVDRVKGSVPMQRGGQ
                     PEEVAEAILWLMSRESSYCTGIFIDVTGGR"
     gene            complement(4178..5581)
                     /locus_tag="VI319_10120"
     CDS             complement(4178..5581)
                     /locus_tag="VI319_10120"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY6864245.1"
                     /translation="MTTVAVPFWLAVLGGLLAAWALYERVMMPSLRWVIRSRANKALD
                     EFGSRLRISIRPFQQTRRQALIDLLAYDAKVQEAVAEHARASGMPRELVTAQAHRYAR
                     EIVPAFNAYLYFRLGYWAARRVAESLYRIRIGYVDERRLEGIPADATVVFVMNHRSNM
                     DYVLATYLAADHAALSYAVGEWARIWPLQQLIRAMGAYFVRRNSRDDLYRRVLERYVA
                     MATENGVTQALYPEGRLTRDGRIGEARLGVLDYMLRGFRNDGERDLVFVPLGINYDRT
                     LEDRSLLLALDPDAKRPGRAGAVWNTLRFAANQASLAFHSKWRRFGYAGVNFGAPISM
                     REYSRSRGLELARLPKEERFAHVAALARHLMDAVGALVPVLPVPLIAAVLCDAPGRRF
                     SEIEIKAAVEALAERAERSGARICVPRPDLDEAVDAGLDTLALRRLVVREDGLYRAAP
                     AESALLRYYANSIAHLL"
     gene            complement(5578..6348)
                     /locus_tag="VI319_10125"
     CDS             complement(5578..6348)
                     /locus_tag="VI319_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY6864246.1"
                     /translation="MTFEIVTDPLFWALAVPAVVLNGISKGGLGGAAGSVSTPLMALA
                     VPPAQAAAILLPVLCVMDLAGIKAYLGKWERRLIFQVMLPAGLLGGLIGTLTFRVMKD
                     DWIRLLLGAIAIGFLAWNLVPKKSSDRRPSDAEGWLWCGLSGFTSFVTHVGSPPLLVW
                     ILPQKLEKAVFVSTLLVFFAAMNWFKIPAYLWLGLLDLRNLWTSVLLVPLGVAGTYLG
                     VWLQTRIDVGVFYRIVYALLLVTGAKLLYDGVAGLWMS"
     gene            complement(6362..7417)
                     /locus_tag="VI319_10130"
     CDS             complement(6362..7417)
                     /locus_tag="VI319_10130"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY6864247.1"
                     /translation="MKKYRIAVIPGDGIGKEVVPEGVRVLEAAGRKFGIEFKWDEFPW
                     SCEYRLKHGRMMPEDGLQTIRNHDAVFFGACGFPGVPDHVSLWELLIPMRRGFDLYVN
                     LRPVRLMPGIQSPLAGRKQGDIDFWVVRENTEGEYSSIGGRIFEGTEREMATQQSTFT
                     RKGVDRILKYAFDLAQRRPKKHLTSATKSNGISITMPYWDERVKAMAAQYPGVKTDQF
                     HIDILTAHFVQHPDWFDVVVGSNLFGDILSDLGPACTGTIGIAPSGNINPEKAYPSVF
                     EPVHGSAPDIYGKKIANPIGQIWSGAMMLEHLGHRDAADAIVRAIERALTEGPRTRDI
                     GGKASTEEVGKAIAAAI"
     gene            complement(7414..>7685)
                     /locus_tag="VI319_10135"
     CDS             complement(7414..>7685)
                     /locus_tag="VI319_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864248.1"
                     /translation="STFEELCAIDREAERLACEIALNPERRAALAARGLELETRYAEL
                     IEGMPADQVPESAAAAIMDAQFIQADCDCVSLRLGDFMKANRLIP"
BASE COUNT         1200 a         2651 c         2697 g         1137 t
ORIGIN      
        1 tcgcgcgaca tgtcgttgaa cgagcgcgac agcgtgcgga tctccgaggg gccggtctcg
       61 ggaacgggct cgggcttctt gccgtggccg atcgcggtcg cggcctcggc gaggcgcttc
      121 agcggacggc tgacgcgcga gacgatgaag tacgcggcga cgagcgacag caccagcgcg
      181 agcgcgcccc agtacagcca ttgcagcgcg atcgcgcgct cgatgcgctc gcgcggcacg
      241 cgcacccagt actcgtcctc gtcgatgtgg aagctgaccc agaagccggg gcggccttcg
      301 cggcggctcg cgaagcgtgt ctgcgcgccg agccgctcgc gcaccgcctc ggcgacgagc
      361 cgcaccatct cgcggtcggg cggcgggacg atgcgctcgc cgggctccga aggatagatc
      421 tcgaggccct cccgctcgga gagatcctcg agcagctggc gccggcgctc gggctgcgcg
      481 ttcacgagcg ccgcgcgcgt gaggttgatc gtgctcacca ggttctgcgc gatcacgcgt
      541 gcgcggggcg ccgcctcgta ggcgcggaag atctggaacc aggcgccggt cgcgacgacg
      601 acgagcagcg cgagcaggag gaacgttcgc cacagcagcg aacgcgggag cagggtcatg
      661 ggaccgggcg gtccgggatc aggaggaggc ttgagcggac ggctcgccgt ccggcacgaa
      721 cacgtagccg aagccccaca cggtctggat gtacgccggc cgcgccgggt cgcgctcgac
      781 cagcttgcgc aggcgcgaca cctgcacgtc gatcgagcgg tcgaacacct cgtactcgcg
      841 gccgcgcgcg agctccatca gcttgtcgcg cgacagcggc gtgcgcgggt gcgtgaccag
      901 caccttgagc agcgcgaact cgccggtcgt gagcgagacc gtcttgccgt ccttctcgag
      961 cgtgcgcgtc gcgaggtcga ctgtcatgct gccgaagcgc accttctccg gctcgctgga
     1021 cggcgcgccc ggcggcaccg gcgccgcgcg ccggcgcagc accgcgtgga tgcgcgcgac
     1081 cagctcgcgc gggttgaacg gcttcggccg gtagtcgtcc gcgcccatct ccaggccgac
     1141 gatgcggtcg acctcctcgc ccttggccgt gagcatgatg atcggcacca tgttcttcgt
     1201 gccgcgcagc cgccggcaga tcgagagccc gtcctcgccg ggcagcatca ggtcgagcac
     1261 catcaggtcg aagcgctcgc ggctcatgat cttgtccatg cccgcggcgt cggacacggt
     1321 gtgcacggtg aacccctgct cggtgagata gcgctgcagc aggtcgcgca gccgcaggtc
     1381 gtcgtcgacg acgagaacgc gggtcttggg ctcggccatg gcactatcgt accttgggcg
     1441 cgaaagtgtg accgggcgcg gcggtaacgg atgatttcat cggcacccgg ggttacagcc
     1501 tgttacaaag gcgccgaggg gtttgatata cgaaggcctt cgcccgcctt acactatccc
     1561 gaccatgaag ccgagccgct cattcctgct cctcgccgcg ctcgcggcgc tcgcctgctc
     1621 gggtccggcg ctcgcgcagc accagggccg gggcggccgc ggcgggcagc cgcagaagca
     1681 acagcaggca cagcagcgcc ccgcgccgcc gccggcccag ggcccgcagc agcacgaggc
     1741 gacctaccgc cagcagccgc agcggcagaa cggccgcatg agcgatgagg agcgccagca
     1801 gctgcggcgc gacatctcgg accacgggcg cgacatctac caggatcgcg gcggcgcgag
     1861 acgctgaccg gcgcgggctc acggcctgcg gaaatctaca gttcccaagc tcgccccgcg
     1921 ggcgtagact gcccgtccgc tttccgcagg gacaacatgc cctcgcgtcg tacattgcag
     1981 tggagcgcgc tgccggttgc ggcctggctc ggcctcgccg cgtccggcgc atgggccgcc
     2041 gacaccgacg ccggcgagcg caagaacgcg ttcacgttcg agaccggcgc cgacgagctc
     2101 ttgaagcgca gccaggacct ccgcatggtg ggtccgcgga acatgttcta tctcgcgacc
     2161 ctgaagaagg acttcggcga tcctcgcggc gtccgcccgt tcgtggctgg gggagtaggg
     2221 ctgatcgcgc cgcgtctgac cggctccatg agccgcaatc ccggcggcag cgccgcgcag
     2281 gccgcggctg gcctgcggta cgagtccggc cgtacgagcg tcatcgccga gtaccgctat
     2341 ctctgggcgc cttcgagcga cctcgatgcc cgcggggaaa tgggtgacgc ccaggggaag
     2401 ctgaacctgg gcggccaggc ctactttctc gggctgggga tcaagttctg atcccgccgg
     2461 tcagtcgacc ttcgcacccg agtccttcac cagccgcgcc cagagctttc cttcctcgcg
     2521 cagccacgcc ccgaactcct ccggggattt cgagggcgcc gtctcggtgc cgtcggtcgc
     2581 gaggatctgc ttgagcttcg ggtctctcag cgcggtgacg gtcgcgtcga acaggcggtt
     2641 gatcacggcg cgcggcgtgc cggcgggcgc gaacatgccg taccagaacg tgtagttgta
     2701 gccgtcgacg ccggcttcct tcatgccggg cagctcgggt acgagcgggc tgcgctcggc
     2761 gctggtcatg gcgaggccgc gcaggcgtcc ggccttcacc tgcggcagca ccgacggcgg
     2821 cgtggcgatg gtgagctgcg tgtcgccggc gagcaccgac tgcaccgcga ggccgccgct
     2881 cttgtacggc acgtgcacca tgtggatgcc ggtcaggctc gcgagcagca ccgtgccgag
     2941 gtgcggcgcc gagccgttgc ccgaggtggc gaagttgagc ttgcccgggt tcttcttcgc
     3001 gtagtcgatc agctcgcgca gcgtgctcgc gggcaccgag ggatgcaccg cgacgacgag
     3061 cggcgaggtc gtcaccagcg tcaccggcac gaagtcgcgc tcggtcacgt agttgatgtt
     3121 cttcatgatg atcgggttga cgaacaccgc cgaggggctc gcgatcatca ccgtgtagcc
     3181 gtcgggcgcc gatttggcga ccagctcggc cgccaggctc gagcccgcgc ccggcttgtt
     3241 gtcgacgatg accggctgcc cgaacgagcg ttgcagcggg tcgctcatcg cgcgcgagat
     3301 gatgtccgac gcgccgcccg gcgcgaagcc gacgacgagg tgcagcggcc ggccgggata
     3361 ttgcgactgc gcaaaggcgc ccgaggccag caatgccccg agccaaaacg cgactgcgcg
     3421 aaacattcag cgtcctcccg tcacatcgat gaaaattccg gtgcagtacg acgactcccg
     3481 cgacatgagc cacaggatcg cctcggccac ctcttcgggc tggccgccgc gctgcatcgg
     3541 aacggaacct ttcacgcggt cgacgcggcc cggctcgccg cccgaggcgt ggatctcggt
     3601 gtagatcacg cccggccgca ccgcgttcac gcggatgcct tcggccgcga cctccttcgc
     3661 aagccccagc gtgaacgagt cgatcgcgcc cttcgacgcg gcgtagtcga cgtactcgcc
     3721 gggcgagccg atgcgcgacg ccgcggagga gacgttcacg atcgcgccgc ccttgccgcc
     3781 gcgcttcgtc gacatgcgcc gcacggcctc gcgcgcgcag atgaacgagc cgacgacgtt
     3841 ggtcgtgagg atgcggccca ggcgcgccgc gtcgatgtcc tcgacgcgga tctggcgctc
     3901 gaggatgccg gtgttgttga ccagcgccga gaccgggccg agcgcgcggt cgcactcctt
     3961 gaaaagacgc acgacgtcgg tttcggagga aacgtcggcg gcgatcgcga ccgcccggcc
     4021 gcccgccttc tcgatgtcgc ggaccacggc ttcggcggca tcgcggttct tcaggtaatt
     4081 gacgcacacg gcccagccgc gcctcgcggc gagcgtcgcg gtcgctgcgc cgatgccgcg
     4141 gcccgcgccg gtgatcagta cgaccttgtc catggattca caacagatgg gctatcgagt
     4201 tcgcatagta tcgcagcagc gccgattccg cgggcgccgc ccggtacagg ccgtcctccc
     4261 ggaccacgag ccggcgcagc gcgagcgtgt cgagccccgc gtcgaccgcc tcgtccaggt
     4321 cggggcgcgg cacgcagatg cgcgcgccgg agcgctcggc gcgctcggcg agcgcctcga
     4381 ccgcggcctt gatctcgatc tcggagaagc ggcgcccggg cgcgtcgcag agcaccgcgg
     4441 cgatcagcgg caccggcagc acagggacca gcgcgccgac cgcgtccatc agatgccgcg
     4501 cgagcgcggc gacgtgcgcg aagcgctcct ctttcggcag gcgcgcgagc tcgaggcccc
     4561 gcgagcggct gtattcgcgc atcgagatcg gcgcgccgaa gttgacgccg gcatagccga
     4621 agcgccgcca tttcgagtgg aaggcgagcg aggcctggtt ggcggcgaag cgcagcgtgt
     4681 tccacacggc gccggcgcgt cccgggcgct tcgcgtccgg gtcgagcgcg agcaggagcg
     4741 agcggtcctc gagcgtgcgg tcgtagttga tgccgagcgg cacgaagacc agatcgcgct
     4801 cgccgtcgtt cctgaagccg cgcagcatgt agtcgagcac gcccagccgc gcctcgccga
     4861 tgcgcccgtc gcgcgtaagc cggccctcgg gatagagcgc ctgcgtcacg ccgttctcgg
     4921 tcgccatcgc gacgtagcgc tcgagcacgc gccggtacag gtcgtcgcgc gaattgcgcc
     4981 gcacgaagta cgcgcccatg gcgcggatga gctgctgcag cggccagatg cgcgcccact
     5041 cgccgaccgc gtacgagagc gccgcgtggt cggcggcgag gtaggtcgcg agcacgtaat
     5101 ccatgttgct gcggtggttc atcacgaaca ccaccgtcgc gtcggcgggg atgccttcga
     5161 ggcgccgctc gtcgacgtag ccgatgcgta tgcggtagag cgactcggcg acgcggcgcg
     5221 cggcccagta gccgaggcgg aagtagagat aagcgttgaa cgcgggaacg atctcgcgcg
     5281 cgtagcggtg cgcctgcgcg gtcaccagct cgcgcggcat gccgctcgcg cgcgcgtgct
     5341 cggcgaccgc ttcctggacc ttcgcgtcgt acgcgagcag gtcgatcagc gcctggcggc
     5401 gcgtctgctg gaacgggcgg atcgagatcc ggagccggct gccgaactcg tcgagcgcct
     5461 tgttcgcgcg cgagcggatg acccagcgca gcgagggcat catcacgcgc tcgtagagcg
     5521 cccaggcggc gagcaggccg ccgagcaccg cgagccagaa cgggacggcg acggtggtca
     5581 tgacatccat aagccggcga cgccatcgta gagcagcttc gcccccgtga cgaggagcag
     5641 ggcgtagacg atgcggtaga acacgccgac gtcgatccgc gtctgcagcc acacgccgag
     5701 gtacgtgccg gcgaccccca gcgggacgag cagcaccgag gtccacaggt tgcgcagatc
     5761 gagcagcccg agccacaggt acgccgggat cttgaaccag ttcatcgccg cgaagaacac
     5821 gagcagcgtc gacacgaaca cggccttctc gagcttctgc ggcaggatcc agacgagcag
     5881 cggcgggctg ccgacgtggg tgacgaagct cgtgaacccg gagaggccgc accagagcca
     5941 tccctcggcg tccgacggcc ggcgatcgga gcttttcttc ggaacgagat tccaggcgag
     6001 aaagccgatt gcgatcgcac cgagcagcag ccggatccag tcgtccttca tcacccggaa
     6061 cgtaagcgtg ccgatcaggc ctccgaggag gccggcgggc agcatcacct ggaagatcag
     6121 ccgccgctcc cacttcccca ggtaggcctt gatcccggcg aggtccatca cgcacagcac
     6181 cggaagcagg attgccgcgg cttgggcggg cggcaccgcg agcgccatga gcggagtgga
     6241 cacgcttccg gcggcgccgc ccaggccgcc cttcgagatt ccgttcagga cgaccgccgg
     6301 aacggcgagc gcccagaaca gcgggtcggt gacgatctcg aaggtcagag cgcgccggcc
     6361 ttcagatcgc cgcggcgatg gctttgccga cctcttcggt cgacgccttg ccgccgatgt
     6421 cgcgcgtgcg cgggccctcg gtcagcgcgc gctcgatcgc gcgcacgatc gcgtccgcgg
     6481 cgtcgcggtg cccgaggtgc tcgagcatca tcgcgcccga ccagatctgg ccgatcgggt
     6541 tggcgatctt cttgccgtag atgtcgggcg ccgagccgtg caccggctcg aacacggagg
     6601 ggtaggcctt ctccgggttg atgttgcccg agggcgcgat gccgatcgtc cccgtgcacg
     6661 cggggccgag gtcggagagg atgtcgccga aaagattcga gccgacgacc acatcgaacc
     6721 agtcggggtg ctgcacgaaa tgcgcggtga ggatgtcgat gtggaactgg tcggtcttca
     6781 cgccgggata ctgcgccgcc atcgccttca cgcgctcgtc ccagtacggc atcgtgatcg
     6841 agatgccgtt cgacttggtc gcggacgtca ggtgcttctt cggccggcgc tgcgccaggt
     6901 cgaaggcgta cttcaggatg cggtcgacgc ccttgcgcgt gaacgtgctc tgctgcgtcg
     6961 ccatctcgcg ctcggtgcct tcgaagatgc gcccgccgat cgacgagtac tcgccctcgg
     7021 tgttctcgcg cacgacccag aagtcgatgt cgccctgctt tcggccggcg aggggagact
     7081 ggatccccgg catcaggcga acgggtctca ggttcacgta gagatcgaag ccccgccgca
     7141 tcgggatcag cagctcccag agcgacacgt gatcgggcac gcccgggaat ccgcacgcgc
     7201 cgaagaagac tgcgtcgtgg ttccgaatgg tttgaagacc gtcctcgggc atcatccgcc
     7261 cgtgcttcag ccggtactcg cagctccagg ggaactcgtc ccacttgaac tcgatgccga
     7321 acttgcggcc cgcggcctcg agcacgcgca cgccttcggg gacgacttcc ttgccgatcc
     7381 cgtcgcccgg gatcacggcg atcctgtatt tcttcatgga atgagacgat tcgctttcat
     7441 gaaatctccc aggcgcaggc tgacgcagtc gcagtcggcc tggatgaact gcgcgtccat
     7501 gatcgcggcg gcggcgctct cggggacctg gtcggcgggc atcccctcga tgagctcggc
     7561 gtagcgcgtc tcgagctcga ggccgcgcgc ggcgagcgcg gcgcgacgct cgggattcag
     7621 cgcgatctcg cacgcgaggc gctcggcctc gcggtcgatc gcgcaaagct cctcgaaagt
     7681 gctag
//
LOCUS       DATDMW010000239        19407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_943957, whole genome shotgun sequence.
ACCESSION   DATDMW010000239 DATDMW010000000
VERSION     DATDMW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19407
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943957"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /gene="ilvN"
                     /locus_tag="VI319_10140"
     CDS             complement(<1..395)
                     /gene="ilvN"
                     /locus_tag="VI319_10140"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY6864249.1"
                     /translation="MRHIVSIMLENEPGALSRVAGLFSARGYNIESLTVAPTEDASLS
                     RMTIVTSGSDDVIEQITKQLNKLVDVVKVVDLSEAPHIERELMLIKVRATGKDREEMK
                     RTSDIFRGRIIDVTENTYTIEMTGTGSKL"
     gene            complement(537..2264)
                     /locus_tag="VI319_10145"
     CDS             complement(537..2264)
                     /locus_tag="VI319_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HEY6864250.1"
                     /translation="MELRAAEVELSGAEIVVRCLQEEGVEVVFGYPGGAVLFIYDELF
                     KQDKVKHVLVRHEQGAAHAADGYSRSSDKVGVCLVTSGPGVTNAVTGIATAYMDSIPM
                     VIITGQVPTHAIGQDAFQECDTVGITRPCVKHNFLVKDIKDLAVTIKKAFYLAKTGRP
                     GPVLVDIPKDVTAQKYPFDYPKTVSMRSYNPVVKGHPGQIKKAAQLLLEAKRPMIYTG
                     GGVILSGAAEQLTRLVRMLGFPCTNTLMGLGGYPGTDPQFTGMLGMHGTYESNMAMQN
                     CDVLLAVGARFDDRVIGNPEHFAQNQRRIIHIDIDPSSISKRVKVDVPIVGNVREVLD
                     DLIKLIEASRDKPDAKALKAWWTQIEQWKAKDCLRYDRSNSIIKPQFVLEKLYEVTGG
                     EAFVTSDVGQHQMWAAQFYKFDKPKRWINSGGLGTMGFGLSAAMGAQLANPGATVACV
                     TGESSIQMCIQELSTCKQYRLPIKIVNLNNKYMGMVRQWQQFFHGNRYSESYMDALPD
                     FVKLAEGYGHRGVLVEKPADVEPALREGFKRKDELVFFDFITDQKENVYPMVPGGKGI
                     SEMILAEEL"
     gene            2424..2990
                     /locus_tag="VI319_10150"
     CDS             2424..2990
                     /locus_tag="VI319_10150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6864251.1"
                     /translation="MASRNELSEFLAGVERRAYKQALFAVRDEEHALDIVQDSMLKLS
                     ERYGNKPAGELPMLFQRILQNTIRDFYRRQKVRSLWTTLLSALAPNREEDDTDPLEIL
                     EVEDASNVGGNPAALVQQSQVLDLIEKELQRLPMRQRQAFLLRYWEELDVAETAAIMG
                     CSQGSVKTHCSRAAHALSASLRAKGVVI"
     gene            2987..3364
                     /locus_tag="VI319_10155"
     CDS             2987..3364
                     /locus_tag="VI319_10155"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEY6864252.1"
                     /translation="MNEQQTAYRIKQMLNRGLDLDPGKVARLREARERALARQRTKAP
                     VPVLAWADNVTGGWGAPSFVLSRVVLPMVLVIFSLVAISQWRETQQATEIEEIDAAVL
                     TGDLPLDAYLDKGFDAWLRRSSQ"
     gene            3340..3894
                     /locus_tag="VI319_10160"
     CDS             3340..3894
                     /locus_tag="VI319_10160"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEY6864253.1"
                     /translation="MAKALLAVALWLCIAFSAAAATPPKKPPSWAQLTPEQQQILAPL
                     APTWDKLEPTRKRKWLGIAKRYPKMQPEEQAKVQRRMQPWASLSPEERQAARERYRKL
                     EKLPPDKRQALRQQWEEYNRLPEQERRKLGAAQRKAAKSATAAGKAPAPSATVAPDAG
                     TAPSQGEPPAPATAPAAPATAAAK"
     gene            3902..4342
                     /locus_tag="VI319_10165"
     CDS             3902..4342
                     /locus_tag="VI319_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY6864254.1"
                     /translation="MPAAPETDLAPAGLGRRLLAMLYDSLLLFAVAFFAGLAFVFVAG
                     DALSPPKRALFQAYLFAVLGGYFVFCWRRGGATLAMRTWRLRLVRADGGPPRLGQACL
                     RYALAWPSIVLGLGVLWALVDRDRQFLHDRLAGTRIVLEAPRAS"
     gene            complement(4339..5412)
                     /gene="lptG"
                     /locus_tag="VI319_10170"
     CDS             complement(4339..5412)
                     /gene="lptG"
                     /locus_tag="VI319_10170"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY6864255.1"
                     /translation="MDTLRRYLAREIYGATLFVFAAFLSLFAFFDLIFEMGEIGKGQY
                     RLQHALAFVALSIPGHVYELFPVAVLIGTLYALSHLASNSEYTVMRVSGLSPWRASLI
                     LFHIGIAFVVATVIFGELVTPAAERAARKLKLSATTGVVAQEFRTGLWVRDDRRFVNV
                     REILPDATLSDVRVYEFDAEYRLVSVSFAEHARYAKNNVWQLTDVQETRFTASGSTVE
                     HIPEVDWTSVLTPELLSVLFVLPERMSAWNLYQYTQHLAENRQKTERYEIAMWKKLVY
                     PFASLVMMALALPFAYVHTRAGGLGAKVFSGIMLGVLFHALNSLFSHLGLLQNWRPVY
                     SAVLPSVLFLATAASMMWWVERR"
     gene            complement(5412..6497)
                     /gene="lptF"
                     /locus_tag="VI319_10175"
     CDS             complement(5412..6497)
                     /gene="lptF"
                     /locus_tag="VI319_10175"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY6864256.1"
                     /translation="MIFQRSILREFGQLALAVFATLFAITLTTQLIRFLGQAAGGKVQ
                     SESVLALLGFSALNYLPVLLSLTLFVTVLMTLSRSYRDSEMVIWFASGQSLTAWIRPV
                     VAFAAPLVLLIALLSLFLSPWAVEKSEDYRRQMESQDDASRVAPGIFREAGGADRVFF
                     VEAVAGDENSVQNVFIAQMRQGKLAIMTSKRGYRETAANGDRFVVLLNGRRYDGAPGT
                     PEYKVMDFERYAIRIETRDPQAEQSSSKAMSTWALIRDRTNGNLAEILWRIGLPLSAM
                     TLALLAIPLSFVNPRASRSVNLIFALLTYMVYSNLISIVQAWVSQGKMSFGTGWWVVH
                     AGMLVVLLLMFYRRLRVGSLLSFLRLR"
     gene            6613..8100
                     /locus_tag="VI319_10180"
     CDS             6613..8100
                     /locus_tag="VI319_10180"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY6864257.1"
                     /translation="MEFSIKVSAPEKQPSACVVVGVFESRKLSPAAEQIDKASKGQVR
                     ALLRAGDMEGKLRSTRMLYRVPGVSAERVLLVGLGAEKELRDKDFGEAARATTRALLE
                     SGSAEAAVYLCEIAVGGRDAEWKARQFAVAAGDSGYRFDLMKSKKADARPLRRIVLAT
                     TSKRDAALLETGVRAGAAITAGMSLARDLGNLPPNVCTPTYLAEEAVKLARQWKLGVE
                     VLEQRDVEKLGMGAFLAVARGSRQPPKFIVLQYSGAAKKDKPVVLVGKGITFDTGGIS
                     IKPAAEMDEMKFDMCGAASVLGALRAAAEMKLKLNVVGLVPTTENMPGGNATKPGDIV
                     KTLSGQTVEILNTDAEGRLILCDALTYAERYDPQAVIDIATLTGACVIALGHVCSGMF
                     TNKDDLAREMIAAAADSSDRVWRLPLWDDYQEQLKSNFADFANIGGRAGGAITAACFL
                     ARFTRKYDWAHLDIAGTAWKSGKEKGATGRPVPLLVAFLAERAKG"
     gene            8097..8513
                     /locus_tag="VI319_10185"
     CDS             8097..8513
                     /locus_tag="VI319_10185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY6864258.1"
                     /translation="MTRIEFYFNADSKLRAACQLVARALERRLRVLIYAPDEAVARGV
                     EEALRDFPAIPFALGAQSGEAAPVLISGEADAARHDEVLLNLHDGAPRHFSRFRQVIE
                     VVARAEDDRQAARERFRYYRDRGYAIRHYDMALGRT"
     gene            8510..8875
                     /locus_tag="VI319_10190"
     CDS             8510..8875
                     /locus_tag="VI319_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864259.1"
                     /translation="MSDPKDIVEKADSFLGRYRPGAAAEVPVLTDIVEVAESARAAGA
                     SSKPGPAAALTEGQLRELEREITRRVLEAIQPALAQLLEPALATLSDQVKAQAEAFVR
                     AAVTNALEREIRLLRPPGG"
     gene            8896..11661
                     /locus_tag="VI319_10195"
     CDS             8896..11661
                     /locus_tag="VI319_10195"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY6864260.1"
                     /translation="MELAKAFDPHEIERRWYPLWETRGYFERSRDASKPPYAIQLPPP
                     NVTGTLHMGHAFQQTLMDALIRYHRMRGFDTNWIVGTDHAGIATQIVVERQLQAEGKT
                     RHDLGREAFVRRVWEWKQQSGSTITRQMRRLGASANWGYADTEGQRGGYFTMDARMSR
                     AVAEVFVRLHEQGLVYRGKRLVNWDPKLGTAVSDLEVDSEEENGRLWEIRYPFGDGAE
                     GVVVATTRPETMLGDVAVAVNPKDARYAALVGRSVRLPLTGRTIPVIADDYVDPEFGT
                     GCVKITPAHDFNDYAVGQRHKLPLISVMTLEARINDNGPAAYRGLDRFEARKRILADL
                     RAQGLLVSEKPYKLRVPRSGRTEEIVEPMLTDQWFVKMDGLAKRGLDAVARGDVRFFP
                     EHWKSWYDNWLENIQDWCISRQLWWGHQIPAWYDEQGNVYVARTEAEAQEKARGRKLT
                     RDPDVLDTWFSSALVPFTSLGWPEKTRDLEVFLPSSVLVTGFDIIFFWVARMIMMTLH
                     FTGQVPFRHVYINALVRDAEGEKMSKSKGNTLDPLDLIDGIDLENLLKKSTTGLLRAD
                     HKEKAEKYVRRHFPNGIPAFGADALRFTFASLATFARTLNFDLDRCEGYRNFCNKLWN
                     ATRFVLMNAEGKDCGQDERAPVTLSTWDRWIVSTLQRTEADVERGFAEYRFDNVAGAI
                     YRFVWDEYCDWYVEIAKTQLAAGSEAEQRGTRRTLVRVLEAALRLAHPIIPFITEELW
                     QTVAPLAGKTGDSIMLAPYPVSQPEKIDKTADEDVATAKDLVNAARNLRAEMKLQPQD
                     QASFYVTGDPSSTAVTAFAALVRQSKLNLVRELPESDSPVAVVGPHRLLLEVQIDPVA
                     ERARIAKEIARIEQDITKCRGKLGNPSFVERAPAQVVQQERERLTGFEATLDKLKQQL
                     AKLQSRP"
     gene            11668..12924
                     /locus_tag="VI319_10200"
     CDS             11668..12924
                     /locus_tag="VI319_10200"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6864261.1"
                     /translation="MPIATASPLRALRHRSFRIFYAGQFLSLIGAWMQTVATSWLVYR
                     LTGSALLLGLTTAAQQFPMLFVAPVAGVWADRVNRRRLLIATQSLAAAQATALTVLTF
                     TGAIQVAHVIALAVVLGVLVALETPTRQAFLLEMVEREDLPNAIALQSMLFQSARFIG
                     PSIAGLMLAAVGEAWCFLVNAVSYLIIVLTYVIIPTKSRSLPSGRSEWWRDLASGFRY
                     AFGFLGTRRLLLLLAAVGALTAPWSPLMPIFAAEILRGDSRTLGFLIGAVGLGALAGT
                     AFLAMRTTVRGLGRVVCVTSITAGIALASFSLSQTLWVSLALLPFFGFGLVATAAASN
                     TILQTVADEDKRGRIIGMYVMTFLGIAPIGNFIAGAAAERFGAPPTLMVCGLLVACAG
                     GCFALGLRSWARAVRPVYSERGIPER"
     gene            complement(12937..13164)
                     /locus_tag="VI319_10205"
     CDS             complement(12937..13164)
                     /locus_tag="VI319_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY6864262.1"
                     /translation="MNYDKELDARGLNCPLPILRTKKALTGMASGQVLRVLATDPGAV
                     KDFQAFARQTGNELLSHAEQDKVYTFFMKKR"
     gene            13272..14243
                     /locus_tag="VI319_10210"
     CDS             13272..14243
                     /locus_tag="VI319_10210"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEY6864263.1"
                     /translation="MAREKEAPASVSQWLVFVAGLPTDDPAVRMRVLRTLESLGCAML
                     REGVYVLPDNPATRQGLTRLSEYVGRISGSSHVLCVTAADPSQAQGFRALFDRSGKYE
                     QLVKTVEGLRSGFGISDPGAIARVLNKQRREFDAISALDFFPSEARERAARALVETEA
                     EVRKLMFPDAPRTGRAADPAASYFKRVWATRRPLWADRLGSAWLIRRFIDPEAKIVWL
                     DKNQDAPSNAVGFAYEGAAFCNSKNKVTFEELLAGFGLERNSTLAKIGTLVHYLDAGG
                     TPVAEAAGVETLLQGARRRSSNEDELFRESEKTFDLLYEAYVETPKA"
     gene            complement(14260..15687)
                     /locus_tag="VI319_10215"
     CDS             complement(14260..15687)
                     /locus_tag="VI319_10215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY6864264.1"
                     /translation="MRKLLASLAFAVAAPVLAQSLPELGDRLSSALSPALERKIGEQA
                     MQDIRLHDPHYLDDPELTEYINALGQRLVAAAPDARLDFQFFIMQDSTINAFAMPGGF
                     VGVHTGLLLAAQTESELAGVLAHEVAHVTQRHLARMLGQQQQMSIPTIAALVVAVLAS
                     RSGGDISQGIIAAAGAGSIQAQLNYTRDYEREADRVGFQILQGANFDVNGMAAFFERL
                     QKATRIYENNAPAYMRTHPLTTERIADMQNRAYHAVYRQSPDSLEFHLVRAKLRAEQG
                     SPRDAKADFADQVRQRRYANEAAARYGLALALSRARDYAGAEAEIARLRTLAGTQPMV
                     ELLAARIKAANGDQAGARDVLQAALAHDPNYRPLSYALVQSLQALGQHERALQLLEEM
                     VRNTPRDPRLYTLKAQSHAALGQRLLQHQAQAEAYYLDGTLSAAIEQLQLAQKSGDAD
                     FYQLSSVDARLRELRRLQADEARKR"
     gene            15799..16293
                     /gene="moaC"
                     /locus_tag="VI319_10220"
     CDS             15799..16293
                     /gene="moaC"
                     /locus_tag="VI319_10220"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY6864265.1"
                     /translation="MRKKKTAARMSHFDTSGQAHMVGVGDKSESLRVARAEGRIRMLP
                     ATLALVLEGRAGKGDVLGVARVAAIQATKRTGDLIPLCHPLALTRVAADFETDTARSV
                     ITLTVTVETVGRTGVEMEALTGVSVGLLTIYDMCKAVDRAMTIENVRLLEKSGGQSGH
                     FRRP"
     gene            complement(16306..17952)
                     /locus_tag="VI319_10225"
     CDS             complement(16306..17952)
                     /locus_tag="VI319_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864266.1"
                     /translation="MNRADIGRLPVWACASVVAALACALYLPFLNNPRVFDDWVFFSG
                     EHFFYYATHPLGLGLRLLPYFTLALTEVTIGGMPAHRLVSLAFHLACSLMVFKLSCNL
                     LRTVSAGDADDESRARTWSMVGASLFALHPVGVYAAGYLVERTIVIATLFSLASLVLF
                     VRGLRRRSHADAVSAALLYSLAVLSKEHSLLLPAAALLTVPLTGAVRGFGIRYAWVYL
                     AACAPAALFAVLVRKGVIGTAYEPYFGLLASQMEDIFGIKDLSLWMSIVTQAALFFRY
                     LAAWLLPDTEAMSIDVRVDFLRTWSPVAIVLESAAFLAYGALGFALWRRGGRTGLVGF
                     GLLYPWVMYIVEFSAARFQEPFVLYRSYLWAPGWALAFAAASSKAPPRAALAAVTLAG
                     ALLFYQARDRLATFSDSLALWKDAVAKLPDKPVPWGSRTLYMAAREYVYSAQPGKAME
                     LAERCLGQYRRSLHCHYARGVIYLLTDQPAKALPDLIRAVELDPQGGITHHRLGLVLE
                     RLGRFDDAKAEYRTAVSLGFAAADFELKRLESARLPNRYR"
     gene            complement(18020..18511)
                     /locus_tag="VI319_10230"
     CDS             complement(18020..18511)
                     /locus_tag="VI319_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY6864267.1"
                     /translation="MKKMQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKMSEVI
                     LAASACRTSITEVFQSGNSASIQAGNWGCEVTSANNPNGASKYVKTIDTTINGLVQVT
                     TQGIGNTAVDNKVVTLLPLRTPTVPAAQSDVGATSLFGWRCGASADGTTVIGKYLPGS
                     CRG"
     gene            complement(18723..>19407)
                     /locus_tag="VI319_10235"
     CDS             complement(18723..>19407)
                     /locus_tag="VI319_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY6864268.1"
                     /translation="VRAGGADARARLNDIDQSLRESLRHIDADRDFLRHCNIGGEQMS
                     PAAQDRVRRIATGYKWTDVSGHTADFLTEDETTNLTIARGTLTGAEREIINHHIVATI
                     KMLEALPWPKHLRNVPEYAGGHHERMDGKGYPRGLTREQMSVQARVMGIADIFEALTA
                     KDRPYKRGKTLSESLKILGTFALGGHIDPDLFDVFIRRKVYLRYAEAFLDKEQIDAVD
                     ELQIPGYAP"
BASE COUNT         3258 a         6506 c         6519 g         3124 t
ORIGIN      
        1 tcgagcttcg accccgtgcc ggtcatctcg atcgtgtagg tgttctcggt cacgtcgatg
       61 atgcggccac ggaagatgtc cgacgtgcgc ttcatctcct cgcgatcctt gcccgtggcg
      121 cgcaccttga tcagcatgag ctcgcgctcg atgtgcggcg cctccgacag atcgacgacc
      181 ttgacgacgt cgaccagctt gttcagctgc ttggtgatct gctcgatcac gtcgtcggag
      241 cccgaggtga cgatggtcat ccgcgagagc gacgcgtcct cggtcggcgc gaccgtgagc
      301 gactcgatgt tgtacccgcg ggccgagaac agtcccgcga cccgggacag cgcgcccggc
      361 tcgttttcca gcatgatgga aacaatatgt ctcatggcga ctccgctgag gcccgaccca
      421 ggcgccgcgt cgccgcgaac gcccctcgac gcggcgctcg tggcgccgcc gagccaggct
      481 cccttcagct cgttgatcaa tccccgcgtg catcgtgcgc gcggggcact gccttgctac
      541 agctcctcgg ccaggatcat ctccgagatg cccttgccgc cgggcaccat cggatagacg
      601 ttttccttct ggtcggtgat gaagtcgaag aacaccagct cgtccttgcg cttgaatccc
      661 tcgcgcaagg cgggctcgac gtcggcgggc ttctccacca gcacgccgcg gtggccgtag
      721 ccttcggcga gcttaacgaa gtcgggcagc gcgtccatgt acgactcgga atagcggttg
      781 ccgtggaaga actgctgcca ctggcgcacc atgcccatgt acttgttgtt caggttgacg
      841 atcttgatcg gcagccggta ctgcttgcag gtcgagagct cctggatgca catctggatg
      901 ctcgactccc cggtcacgca ggcgaccgtc gccccgggat tcgcgagctg cgcgcccatc
      961 gccgcggaca ggccgaagcc catcgtgccg aggccgcccg agttgatcca gcgcttgggc
     1021 ttgtcgaact tgtagaactg cgccgcccac atctggtgct gaccgacgtc ggaggtgacg
     1081 aaagcttccc cgcccgtcac ctcgtagagc ttctccagca cgaactgcgg cttgatgatc
     1141 gagttcgagc ggtcgtagcg caggcagtcc ttggccttcc actgctcgat ctgcgtccac
     1201 caggctttga gggccttggc gtcgggcttg tcgcggctcg cctcgatcag cttgatcagg
     1261 tcgtcgagga cctcgcgcac gttgccgacg atcggcacgt cgaccttgac gcgcttggag
     1321 atcgacgacg ggtcgatgtc gatgtggatg atgcgccgct ggttctgcgc gaagtgctcc
     1381 gggttgccga tcacgcgatc gtcgaagcgg gcgcccaccg cgagcagcac gtcgcagttc
     1441 tgcatcgcca tgttcgactc gtaggtgccg tgcatcccga gcatgccggt gaactgggga
     1501 tcggtgcccg ggtagccgcc gaggcccatg agggtgttcg tgcacgggaa accgagcatg
     1561 cgcaccaggc gcgtgagctg ctccgcggcg ccggagagga tcacgccgcc gccggtgtag
     1621 atcatcggcc gcttggcctc gagcagcagt tgcgccgcct tcttgatctg tcccgggtga
     1681 cctttcacga ccgggttgta cgagcgcatg ctgaccgtct tcggataatc gaacggatat
     1741 ttctgagccg tcacgtcctt cgggatgtca accagcaccg gccccgggcg cccggtcttg
     1801 gcgaggtaga acgccttctt gatggtgacg gcgaggtcct tgatgtcctt caccaggaag
     1861 ttgtgcttca cgcacgggcg cgtgatgccg accgtgtcgc attcctggaa cgcgtcctgg
     1921 ccgatggcgt gcgtgggcac ctggccggtg atgatcacca tcgggatcga gtccatgtag
     1981 gcggtcgcga tcccggtcac cgcgttggtc acgccggggc ccgaggtcac caggcatacg
     2041 ccgaccttgt cgctcgagcg cgagtagccg tcggcggcgt gcgccgcgcc ctgctcgtgc
     2101 cggaccagca cgtgcttcac cttgtcctgc ttgaacagct cgtcgtagat gaacaggacc
     2161 gcgcccccgg gatagccgaa gacgacctcg acgccttctt cctgcaggca gcgcacgacg
     2221 atctcggcgc cagacagctc cacttcggcg gccctcagtt ccattccgga tccttgtatt
     2281 tctcgagcgg aaaattttgc agagtaaatg ctagcatctg attggtcaag ttttttcccg
     2341 cttctcgggc ggcggggccc gcgtgttttg gtttaacatc gcccacaaac agcagccgcc
     2401 cccgcacaaa aaaggaagcg ctcctggcct cccgaaacga gctctcggag ttcctcgccg
     2461 gcgtcgagcg gcgggcgtac aagcaggcgc tgttcgcggt acgcgacgaa gagcacgcgc
     2521 tcgacatcgt gcaggactcg atgctcaagc tctccgagcg ctacggcaac aagccggccg
     2581 gggagctgcc gatgctgttc cagcgcatcc tgcagaacac gatccgcgat ttctaccggc
     2641 gccagaaagt ccggtcgctc tggacgacgc tgctctcggc gctcgcgccc aaccgggagg
     2701 aagacgacac ggatccgctg gaaattctcg aggtcgagga tgcatccaat gtgggtggaa
     2761 acccggccgc gctggtacag caatcgcaag tacttgactt aatagagaaa gaactgcagc
     2821 gcctccccat gcgtcaacgc caggcgtttc tcctgcgtta ctgggaagag ttggacgttg
     2881 ccgagaccgc ggccataatg ggctgctcgc aaggcagcgt gaagacccat tgctcgcgcg
     2941 ctgcgcacgc gctgtcggcc tcgctgcgag cgaagggggt cgtgatatga acgaacagca
     3001 aaccgcctac cggatcaagc agatgctgaa ccggggcctc gacctggacc ctgggaaggt
     3061 cgcgcgcctc agggaagcgc gcgagcgtgc ccttgcgcgc cagcgcacga aagcgcccgt
     3121 cccggtcctc gcctgggccg acaacgtcac gggtggatgg ggcgctccgt cattcgtgct
     3181 gtcgcgcgtg gtcctgccca tggtgctggt catcttcagc ctggtcgcca tcagtcaatg
     3241 gcgcgaaacg cagcaggcga ccgagatcga ggagatcgat gccgcggtgc tcaccggcga
     3301 cctgccgctc gacgcgtatc tcgacaaagg gttcgatgca tggctaaggc gctcctcgca
     3361 gtagccctct ggctctgcat cgcgttttcc gccgccgccg cgaccccgcc gaagaagccg
     3421 cctagctggg cgcagctgac gcccgagcag cagcagattc tcgcgcccct cgctccgacc
     3481 tgggacaagc tcgagcccac gcgcaagcgc aagtggctcg gcatcgccaa gcgctatccg
     3541 aagatgcagc ccgaggagca ggccaaggtc cagcggcgca tgcagccctg ggccagcctc
     3601 tcccccgagg agcggcaagc cgcgcgcgaa cgctacagga agctcgagaa gctgccgccg
     3661 gacaagcggc aggctttgcg tcagcaatgg gaggaataca accgcctccc cgagcaggag
     3721 cgacgcaagc tcggcgccgc ccagcgcaag gccgcgaaat ccgcgactgc ggccggcaag
     3781 gcgccagcgc cctcggcgac ggtcgctccc gacgccggca cggcgccgtc ccagggcgag
     3841 ccgcccgcgc cggcgaccgc tcccgcggcg cccgccaccg ccgccgcgaa atagccgcgc
     3901 gatgccggcg gcgccggaga cggacctcgc ccccgccgga ctcggccgtc gcctgctcgc
     3961 catgctgtac gactcgctcc tcctcttcgc ggtggcgttc ttcgccgggc tcgctttcgt
     4021 cttcgtggcc ggcgatgcgc tgtcgccgcc caagcgggcg cttttccagg cgtacctctt
     4081 cgcggtgctc ggaggatatt tcgtcttttg ctggcgccgg ggcggtgcca cgctcgccat
     4141 gcggacgtgg cggctgcggc tggtgcgcgc cgacggcggg ccaccgcggc tcggccaggc
     4201 ctgcctgcgc tacgcgctcg cttggccctc gatcgtgctc ggcctgggcg ttctctgggc
     4261 gctcgtcgac cgcgaccgcc agttcctcca cgaccgtctc gccggcacgc gcatcgtgct
     4321 cgaggcgccg cgcgcgagct agcggcgctc cacccaccac atcatcgacg ccgccgtcgc
     4381 caggaacagc acgctcggca agaccgcgct gtacacgggc cgccagttct gcagcagccc
     4441 caggtgcgag aacaggctgt tcagtgcatg gaacagcacc ccgagcatga ttcccgagaa
     4501 gaccttggcg ccgagcccgc cggcgcgcgt gtggacgtag gcgaacggca gcgccagcgc
     4561 catcatcacc agggacgcga acgggtacac gagcttcttc cacatggcga tctcgtagcg
     4621 ctccgtcttc tgccggttct cggccaggtg ctgggtgtac tggtagaggt tccaggcgga
     4681 catgcgctcg ggcaggacga agagcacgga gaggagctcg ggcgtgagca ccgaggtcca
     4741 gtcgacctcg ggaatgtgct cgaccgtgct tcccgaggcg gtgaagcgcg tctcctgcac
     4801 gtcggtgagc tgccagacgt tgttcttcgc gtaacgcgcg tgctcggcga agctgaccga
     4861 caccaggcga tattccgcgt cgaactcgta gacgcgcacg tcggacaagg tggcgtcggg
     4921 cagtatctcg cgcacgttga cgaagcggcg gtcatcccgc acccaaagcc cggtgcggaa
     4981 ttcctgcgcc acgacgccgg tcgtggccga gagcttgagc ttgcgcgccg cacgctcggc
     5041 ggcgggagtc acgagctcgc cgaagatcac cgtcgccacg acgaacgcga tgccgatgtg
     5101 gaagaggatc agcgatgcgc gccacgggga caggccggat acgcgcatga ccgtgtattc
     5161 ggagttggac gcgaggtggg acagcgcata aagcgtcccg atcagcaccg cgaccgggaa
     5221 cagctcgtac acgtgccccg ggatcgagag cgcgacgaac gcgagcgcgt gctgcaggcg
     5281 gtattggcct ttcccgatct cgcccatctc gaaaatcagg tcgaagaacg cgaacaggct
     5341 gaggaaggcc gcgaacacga acagcgtcgc gccatagatc tcccgcgcca ggtagcgccg
     5401 cagcgtgtcc atcagcgcag cctcagaaag gaaagcagcg atccgacccg cagccggcgg
     5461 tagaacatca gcagcagcac caccagcatt ccggcgtgca cgacccacca accggtgccg
     5521 aacgacatct tgccctggga aacccaggcc tggacgatgc tgatgagatt gctgtacacc
     5581 atgtaggtga ggagcgcgaa gatgaggttc accgagcggc tggcccgcgg attgacgaac
     5641 gagagcggta tggcgagcag cgcgagcgtc atcgcggaca gcggcagccc gatccgccac
     5701 aggatttccg cgaggttgcc gttggtacgg tcccggatca gggcccaggt cgacatcgcc
     5761 ttgctcgagg actgctcggc ctggggatcg cgggtctcga tgcgtatggc gtagcgctcg
     5821 aaatccatca ccttgtactc gggcgttccg ggcgcgccgt cgtagcggcg gccgttcagc
     5881 agcacgacga agcggtcgcc gttcgccgcg gtctcgcggt agccgcgctt gctggtcatg
     5941 atggcgagct tgccctggcg catctgggcg atgaagacgt tctgcacgct gttctcgtcg
     6001 cccgcgaccg cctcgacgaa gaacacgcgg tcggcgccgc ccgcctcgcg gaatatgccg
     6061 ggggcgacgc gcgaggcgtc gtcctgcgat tccatctggc ggcggtaatc ctcgcttttt
     6121 tccaccgccc agggcgagag gaaaagcgac agcagcgcga tcagcaagac cagcggcgca
     6181 gcgaaggcca ccaccggcct gatccaggcg gtaagcgact ggcccgacgc gaaccagatc
     6241 accatctcgg aatcacggta gctgcgcgac agcgtcatga gcaccgtgac gaagagcgtg
     6301 agcgacagca gcaccggcag ataattcagc gccgagaatc cgagcagcgc gagcacgctc
     6361 tccgactgca ccttgccgcc tgccgcctgg ccgaggaagc ggatcagctg cgtcgtcagc
     6421 gtgatggcga acaacgtcgc gaacaccgcg agcgccagct ggccgaactc gcggaggatg
     6481 gaacgctgga agatcaccgg aaaacgctgc tgctcgtcgc ggaagaccgc ccgaatcgtc
     6541 gcgcgctgct ttgactttgt cggagcaaag cggagataat tccgcgctgg ttctcagggg
     6601 tcgaggtcgt ccgtggaatt tagcataaaa gtgtcggcgc cggagaagca gccgtcggcc
     6661 tgcgtcgtcg tcggcgtgtt cgaatcgcgc aagctgtcgc cggccgcgga gcagatcgac
     6721 aaggcgtcga aggggcaggt ccgcgcgctg ctgcgcgcgg gcgacatgga aggcaagctg
     6781 cgcagcacgc gcatgctcta ccgggttccg ggggtttccg cggagcgcgt gctgctggtc
     6841 ggcctcggcg cggaaaagga gctccgcgac aaggatttcg gcgaggccgc ccgcgccacc
     6901 acgcgcgcgc tgctcgaaag cggctcggcc gaagctgcgg tctacctttg cgagatcgcg
     6961 gtcgggggtc gcgacgccga gtggaaggcc cggcagttcg ccgtcgcggc gggcgactcg
     7021 ggctatcgct tcgatctcat gaaaagcaag aaggccgacg cgcgtccgct gcgccgaatc
     7081 gtgctcgcca ccacctcgaa gcgcgatgcc gcgctcctcg agaccggggt gcgggcgggc
     7141 gccgcgatca cggccggaat gtcgctcgca cgcgacctgg gcaacctgcc gccgaacgtc
     7201 tgcacgccga cctacctggc cgaagaagcc gtgaagctcg cccgccaatg gaagctgggc
     7261 gtggaagtgc tcgagcagag agacgtggag aagctcggca tgggcgcctt cctcgccgtc
     7321 gcccgcgggt cgcgccagcc gccaaaattc atcgtgctgc agtattccgg ggcagcgaaa
     7381 aaggacaagc ccgtggtgct cgtcggcaag ggcatcacgt tcgacaccgg cggcatctcg
     7441 atcaagccgg cggccgagat ggacgagatg aagttcgaca tgtgcggtgc cgcgagcgtg
     7501 ctcggcgcgc tgcgcgccgc ggccgagatg aagctcaagc tcaacgtggt ggggctcgtc
     7561 ccgacgaccg aaaacatgcc gggaggaaac gccacgaagc ccggcgacat cgtgaagacc
     7621 ctttcggggc agacggtcga gatcctgaac accgacgccg agggccgcct cattctgtgc
     7681 gacgctctca cgtacgccga gcgctacgat ccgcaggcgg tgatcgacat cgcgacgctc
     7741 acgggtgcct gcgtgatcgc gctcgggcac gtgtgctcgg gaatgttcac caacaaggat
     7801 gacctcgcgc gcgagatgat cgctgcggcg gcggattcgt cggaccgggt gtggcgattg
     7861 ccgctgtggg acgactacca ggagcagctg aagagcaact ttgcggactt cgccaacatt
     7921 ggcggacgcg ccgggggcgc catcaccgcg gcgtgctttc tcgcccggtt cacccgcaag
     7981 tacgactggg cccacctcga catcgccggc acggcgtgga aatccggcaa ggaaaagggc
     8041 gcgaccgggc gacccgtgcc gctgctggtc gcgttcctgg ccgagcgggc caagggatga
     8101 cccggatcga gttctatttc aacgcggatt ccaagttgcg cgcggcctgc cagctggtgg
     8161 cccgggcgct cgagcggcgg ctgcgcgtgc tgatctatgc tcccgacgag gccgtcgcgc
     8221 gcggcgtgga ggaggcgctg cgcgactttc cggcaatccc cttcgcgctc ggtgcgcaaa
     8281 gcggcgaggc ggcgcccgtg ttgatctcgg gcgaggccga cgcggcccgc cacgacgagg
     8341 tgctgctcaa tctgcacgac ggcgccccgc gtcacttcag ccgcttccgg caggtgatcg
     8401 aggtggtcgc ccgcgccgag gacgaccggc aggcggcgcg agagcgcttc cgctactacc
     8461 gcgatcgcgg ctacgcgatc cggcattacg acatggcgct cgggcgcacg tgagcgaccc
     8521 caaggacatc gtcgagaagg ccgattcctt cctcggccgc taccggcccg gtgcggccgc
     8581 ggaggtcccc gtcctcaccg acatcgtcga ggtcgccgag tcggcgcgtg cggcgggcgc
     8641 gtcctcgaag ccggggccgg cggccgcgct caccgaaggg cagttgcgcg agctcgagcg
     8701 cgagatcacg cggcgcgtcc tcgaggcgat tcagccggca ctcgcgcagt tgctcgagcc
     8761 ggcgctcgcg acactgagcg atcaggtcaa ggcccaggcc gaggccttcg tgcgcgctgc
     8821 ggtcaccaac gctctcgagc gcgagatcag gctgctgcgt ccgccgggcg gctagtcggt
     8881 ttccttccgg gctccatgga actcgccaag gccttcgatc cgcacgaaat cgagcggcgc
     8941 tggtatccgc tctgggaaac gcgcggctac ttcgagcgct cgcgggacgc atccaagccg
     9001 ccgtacgcca tccagctgcc gccgccgaac gtcaccggca cgctgcacat ggggcacgcg
     9061 ttccagcaga cgctgatgga cgccctcatc cgctatcacc ggatgcgcgg cttcgacacc
     9121 aactggatcg tcggcacgga ccatgcgggc atcgcgactc agatcgtcgt ggagcgccag
     9181 ctccaggccg aagggaagac gcggcacgac cttggccggg aggccttcgt gcgacgcgtc
     9241 tgggaatgga agcagcaatc gggctcgacc atcacgcgcc agatgcggcg cctcggcgcc
     9301 tcggcgaact ggggctacgc cgacaccgag gggcagcgcg gcggctactt cacgatggat
     9361 gcgcgcatgt cgcgcgcggt cgccgaggta ttcgtccggc tgcacgagca ggggctcgtc
     9421 taccgcggca agcggctcgt gaactgggat ccgaaactcg gcaccgcggt ttcggatctg
     9481 gaggtcgaca gcgaggaaga gaacggcagg ctctgggaga tccgctatcc gttcggggac
     9541 ggcgcggaag gcgtcgtcgt cgcgaccacg cgtcccgaga ccatgctcgg cgacgtcgcc
     9601 gtcgccgtga accccaagga cgcgcgctac gcggcgctcg tcggcaggag cgtacggctg
     9661 ccgctcacgg ggcgcaccat cccggtcatc gccgacgatt acgtcgaccc cgagttcggc
     9721 acggggtgcg tgaagatcac gcccgcgcac gacttcaacg actacgccgt cggccagcgg
     9781 cacaagctgc cgctcatcag cgtcatgacg ctcgaggcgc gcatcaacga caacgggccc
     9841 gcggcgtacc gcggtctcga ccgcttcgag gcacgcaagc gcatcctcgc cgacctgcgc
     9901 gcgcaggggc tgctcgtctc ggagaagccg tacaagctgc gcgttccgcg atcgggacgc
     9961 accgaggaga tcgtcgagcc catgctcacg gaccagtggt tcgtgaagat ggacgggctc
    10021 gcgaagcgcg ggctcgacgc cgtggcgcgc ggcgacgttc gcttcttccc cgagcactgg
    10081 aagagctggt acgacaactg gctcgagaac atccaggact ggtgcatctc gcgccagctg
    10141 tggtggggtc accagatccc ggcctggtac gacgagcagg ggaacgtcta cgttgcgcgc
    10201 acggaggccg aggcccagga gaaggcacgc ggccgcaagc tcacgcgcga cccggacgtg
    10261 ctcgacacct ggttctcctc ggcgctggtg ccgttcacct cgctcggctg gccggagaag
    10321 acccgcgatc tcgaggtctt cctgccctcg tcggtgctcg tcaccggctt cgacatcatc
    10381 ttcttctggg tcgcccggat gatcatgatg acgctgcact tcacgggaca ggtcccgttc
    10441 cggcacgtgt acatcaacgc cctggtgcgc gacgccgagg gcgagaagat gtcgaagtcc
    10501 aagggcaaca cgcttgaccc gctggatctc atcgacggca tcgacctcga gaacctgctg
    10561 aagaaatcga ccaccgggct gctgcgcgcc gatcacaagg aaaaggccga gaagtacgtc
    10621 cgccgtcact tcccgaatgg gattccagcc ttcggcgccg acgccctgcg cttcaccttc
    10681 gcgtcgctcg cgaccttcgc gcgcacgctc aacttcgacc tcgaccgctg cgagggttac
    10741 cgcaacttct gcaacaagct ctggaacgcg acccggttcg tgctcatgaa cgccgagggc
    10801 aaggactgcg gccaggacga gcgcgcgccg gtgacgctct cgacctggga ccgctggatc
    10861 gtcagcaccc tgcagcgcac cgaggccgac gtcgagcgcg gcttcgccga ataccgcttc
    10921 gacaacgtcg cgggggccat ctaccggttc gtctgggacg agtactgcga ctggtacgtc
    10981 gagatcgcga agacgcagct cgcggccggc tccgaggccg agcagcgcgg cacgcgccgc
    11041 accctcgtgc gcgtgctcga agcggcgctg cgcctcgcgc acccgatcat cccgttcatc
    11101 accgaggagc tctggcagac ggtcgcgccg ctcgcgggga agaccggcga cagcatcatg
    11161 ctggcgccct acccggtgag ccaacccgag aagatcgaca agacagcgga cgaggacgta
    11221 gcgacggcaa aggacctcgt gaacgcggcg aggaatctgc gcgccgagat gaagctgcag
    11281 ccgcaggacc aggcatcgtt ctacgtcacc ggggatcctt cgagcactgc cgtcacggcg
    11341 tttgccgccc tggtgcggca gtcgaagctg aatctcgtcc gggagctacc ggagtccgac
    11401 tcgccggtgg ccgtggtagg gccgcaccgg ctgcttctgg aagtgcagat cgaccctgtt
    11461 gccgagcgcg cgaggatcgc caaggagatc gcgcgcatcg agcaggacat cacgaagtgc
    11521 cgcggaaaac tcggcaatcc ttccttcgtc gagcgagccc cggcgcaagt cgttcagcag
    11581 gagcgcgagc gcctgactgg cttcgaagcc acgctcgata agctgaagca acagctcgcc
    11641 aagctgcaga gccgtccctg agccgccgtg ccgatcgcca cggcgtcccc cctgcgcgcg
    11701 ctgcgccacc gcagcttccg catcttctac gccgggcagt tcctgtctct gatcggcgcc
    11761 tggatgcaga cggtggcgac gagctggctc gtgtaccggc tcacagggtc ggcgctgctg
    11821 ctgggcctga cgaccgccgc gcagcagttt ccgatgctgt tcgtcgcgcc cgtggcgggg
    11881 gtgtgggccg accgcgtgaa ccggcgccgg ctcctgatcg cgacgcagtc gctcgcggcc
    11941 gcgcaggcga ccgcgctcac cgtgctcacg ttcaccggcg cgatccaggt ggcccacgtg
    12001 atcgcgctcg cggtcgtgct cggcgttttg gtcgcgctcg agaccccgac gcggcaggcc
    12061 ttcctgctcg agatggtgga gcgcgaggac ctgccgaacg ccatcgcgct gcagtcgatg
    12121 ctgttccagt cggcacggtt catcggtccc tcgatcgccg ggttgatgct cgcggccgtc
    12181 ggcgaggcgt ggtgcttcct cgtgaacgcg gtgtcttacc tgatcatcgt gctgacctat
    12241 gtgatcatcc cgaccaagtc gcggagcctg ccctcgggac gctccgagtg gtggcgcgac
    12301 ctcgcctcgg gctttcgcta tgcgttcggc ttcctcggga cacggcggct gctgctgctg
    12361 ctcgcggcgg tcggcgccct gacggcgccg tggtctccgc tgatgcccat cttcgcggcc
    12421 gagatcctgc gcggggactc gaggacgctc ggcttcctga tcggcgcagt gggcctgggc
    12481 gcgctcgccg gcacggcgtt cctcgcgatg cgcacgaccg tgcgcgggct ggggcgcgtg
    12541 gtgtgcgtca cgtcgatcac ggccggaatc gcgctcgcga gcttctcgct ctcgcagacg
    12601 ctgtgggtct cgctcgcgct gctcccgttc ttcggcttcg ggctggtcgc gacggcggcc
    12661 gcgtcgaaca ccatcctgca gaccgtcgcc gacgaggaca agcgcgggcg catcatcggg
    12721 atgtacgtga tgaccttcct cggcatcgcg ccgatcggca atttcattgc cggcgcggcg
    12781 gccgagcgct tcggggcccc gccgacgctg atggtctgcg ggctgctcgt cgcgtgcgcc
    12841 ggaggatgct tcgcgctcgg cctgcggagc tgggctcgcg cggtgcgccc ggtctattcc
    12901 gagcgcggca taccggaacg ctagcgcggc gccggcctaa cgcttcttca tgaagaaggt
    12961 atagaccttg tcctgctcgg catgcgagag cagctcgttc ccggtctgcc tggcgaaagc
    13021 ctggaaatcc ttcaccgcgc ccggatccgt ggcgagcacg cgcagcacct ggccgctggc
    13081 catgccggtc agcgccttct tggtgcgcag gatcggcagc gggcaattca gcccccgcgc
    13141 atccagttcc ttgtcgtaat tcatcgtggc cgaatatatc aataatcggg acgccccgta
    13201 acgggccggc aatgcggaca actcgtccgc gatgtgccaa acttgaagcg caacaaggga
    13261 gacggacaaa ggtggcgagg gaaaaggaag ccccggcttc ggtctcgcag tggctggtgt
    13321 tcgtggcggg cctgccgacc gacgatccgg cggtgaggat gcgggtgctc cgcaccctcg
    13381 aatcgctcgg ttgcgcgatg ctgcgcgaag gcgtctatgt cctgccggac aatcccgcca
    13441 cgcgccaggg cttgactcgt ctgtccgagt acgtcgggcg catcagcgga tcttcgcacg
    13501 tgctgtgcgt gacggccgcc gacccgtccc aggcgcaagg ctttcgcgcc ctcttcgacc
    13561 gcagcggcaa gtacgagcag ctggtcaaga ccgtcgaggg cctgcgctcg ggcttcggca
    13621 tctccgatcc gggcgccatc gcgcgcgtgc tgaacaagca gcgccgagag ttcgatgcga
    13681 tcagcgccct cgacttcttt ccgtccgagg cgcgcgagcg cgccgcgcgc gcgctggtcg
    13741 agaccgaagc cgaggtgcgc aagctcatgt ttcccgacgc tccgcgcacc ggtcgcgccg
    13801 ccgatcccgc agccagctac ttcaagcgcg tctgggccac gcgccgtccc ttgtgggccg
    13861 atcgcctcgg ctccgcctgg ctcatccggc gcttcatcga cccggaagcc aagatcgtgt
    13921 ggctggacaa gaaccaggac gccccgtcga acgccgtcgg cttcgcgtac gagggcgccg
    13981 ccttctgcaa cagcaagaac aaggtaacgt tcgaagagct cctcgccggc ttcggactgg
    14041 agcgcaattc gacgctcgcg aagatcggca cgctggtcca ctatctcgac gcgggcggga
    14101 ctccggtcgc cgaagccgcg ggggtcgaga cactgctgca gggcgcgcgc cgacgcagca
    14161 gcaacgagga cgagctgttc cgcgaaagcg agaagacctt cgacctgctc tacgaggcct
    14221 acgtcgagac gcccaaagcg tagcgtcccg gcgcgcgcgc tagcgctttc tcgcctcgtc
    14281 cgcctgcagc ctgcgcagct ccctcagccg cgcgtccaca ctcgagagct gatagaagtc
    14341 cgcgtcgccg ctcttctgcg cgagttgcag ctgctcgatc gccgccgaca gcgtgccgtc
    14401 cagataatac gcttcggcct gcgcctggtg ctgcagcagc cgctgcccga gcgccgcgtg
    14461 ggactgcgcc ttcagcgtat agagccgcgg atcgcgcggc gtattgcgga ccatctcctc
    14521 gagcagctgc agcgcgcgct cgtgctggcc gagggcctgg agcgactgca cgagcgcgta
    14581 actcagcggc cggtagttcg ggtcgtgggc aagcgcggcc tgcagcacgt cgcgcgcgcc
    14641 ggcctggtcg ccgttggcgg ccttgatgcg cgcggcgagc agctcgacca tgggctgcgt
    14701 gcccgcgagc gtgcgcaggc gcgcgatctc cgcttcggcg cccgcgtagt cgcgcgcgcg
    14761 gctgagcgcg agcgccagcc cgtagcgcgc agccgcctcg ttggcgtagc gccgctggcg
    14821 cacctggtcg gcgaagtccg ctttcgcgtc gcgcggtgat ccctgctcgg ctcgcagctt
    14881 cgcgcgcacg aggtgaaatt cgaggctgtc cgggctctgc cggtagacgg cgtggtaggc
    14941 gcggttctgc atgtccgcga tgcgctcggt cgtcaacggg tgggtgcgca tgtacgcggg
    15001 ggcgttgttt tcgtagatcc gcgtcgcttt ctgcagccgc tcgaagaacg ccgccatgcc
    15061 gttcacgtcg aaattggccc cctgcaggat ctggaatccc acccggtcgg cctcgcgctc
    15121 gtagtcgcgg gtgtagttca gctgggcctg gatgcttccc gcgccggcgg ccgcgatgat
    15181 gccctggctg atgtctccgc cggagcgcga tgcgagcacc gcgaccacca gcgccgcgat
    15241 ggtcggaatg gacatctgct gctgctgtcc cagcatgcgc gcgaggtggc gctgcgtcac
    15301 gtgcgcgacc tcgtgcgcga gcacgccggc gagctccgat tccgtctgcg cggcgagcag
    15361 gagaccggta tgcacgccca cgaacccgcc aggcatcgcg aacgcgttga tcgtgctgtc
    15421 ctgcatgatg aagaactgga aatcgaggcg cgcatccggc gccgcggcga cgagccgctg
    15481 gcccagggcg ttgatgtatt cggtcagctc ggggtcgtcc aggtaatgcg gatcgtgcag
    15541 gcgtatgtcc tgcatggcct gctcgccgat cttgcgctcg agcgccgggg acagcgcgct
    15601 cgacaggcga tcgccgagct cgggcagcga ctgcgccagg accggcgccg ccacggcgaa
    15661 ggcgagcgag gccagcaatt tcctcatgcc cgtaatgata ccccgggcga cggccgggac
    15721 gacgcgcagc cgacgaagtg gcgcatcggc cgcgccgttg gcacaatggc gcctctgcga
    15781 ccaccacgtc ggatgctcat gcgcaagaag aaaaccgcag cgcgaatgtc acatttcgat
    15841 acaagtggcc aggcccacat ggtgggcgtg ggcgacaagt ccgaatcgct tcgcgtggcc
    15901 cgcgcggaag gccggatccg catgctcccc gccacgctcg cgctcgtgct cgaaggccgc
    15961 gcgggaaaag gcgacgtgct cggcgtggcg cgcgtcgcgg cgattcaggc caccaagcgc
    16021 accggagatc tcatcccttt gtgccatccg ctcgccctga cgcgggtcgc cgcggacttc
    16081 gaaaccgaca ccgccagatc cgtgatcacg ctcaccgtca ccgtggagac cgtcgggcga
    16141 accggcgtcg aaatggaagc cctcaccggc gtcagcgtcg ggctgctcac catctacgac
    16201 atgtgcaagg cggtcgaccg cgccatgacg atcgagaacg tgaggctgct ggagaaaagc
    16261 ggcggacaat cgggtcattt ccggagaccc tgaccgcgcc gcctgtcagc gataacgatt
    16321 cggcaatcgc gcggattcca gccgcttcaa ctcgaagtcc gcggccgcga agccgaggct
    16381 gaccgcggta cggtactcgg ccttggcatc gtcgaagcgg ccgagacgct cgaggacgag
    16441 cccgagtcgg tggtgggtga tgccgccctg gggatcgagc tcgacggcgc ggatcaggtc
    16501 gggcagcgcc ttggccggct ggtcggtgag caggtagatc actccgcgcg cgtagtgaca
    16561 atgcaaggag cgccggtact ggcccaggca gcgttcggcg agctccatcg ccttgcccgg
    16621 ttgcgcgctg tagacgtatt cccgggccgc catgtacagc gtgcgcgagc cccacggcac
    16681 gggcttatcg ggaagcttcg ccacggcgtc cttccatagc gcgagcgagt ccgaaaaagt
    16741 cgctaggcgg tcgcgcgcct gatagaacag cagcgcgccc gccagcgtta cggccgcgag
    16801 cgccgcgcga gggggcgcct tgctcgacgc ggccgcgaac gccagcgccc atccgggcgc
    16861 ccagagatag ctgcgataga gcacgaacgg ctcctggaac cgtgccgcgc tgaactccac
    16921 gatgtacatc acccatggat acagcaggcc gaatcccacg agcccggtcc tgccgccgcg
    16981 cctccacagc gcgaacccga gcgcgccgta cgcgaggaac gccgccgatt cgaggacgat
    17041 ggcaacgggc gaccaggtgc gcagaaaatc gacacgaacg tcgatggaca tcgcctccgt
    17101 atcgggtagg agccaggcgg cgaggtacct gaaaaacagc gccgcctgcg tgacgatgct
    17161 catccagagc gagaggtcct tgatgccgaa gatgtcctcc atctgcgacg ctaggaggcc
    17221 gaagtacggc tcgtacgccg taccgatcac gcctttgcgc acgagcactg cgaagagggc
    17281 ggcaggcgcg cacgccgcca ggtagaccca ggcatagcgg attccgaagc cgcggaccgc
    17341 gcctgtgagc ggcaccgtga gcagcgcggc ggcaggcaac aggaggctgt gctccttcga
    17401 caggacagcg agggagtaca ggagcgccgc cgagaccgcg tcggcatggc tgcgacggcg
    17461 cagaccgcgg acgaacagca cgagggaggc gagcgagaac agcgtcgcga tcacgatcgt
    17521 ccgctcgacc agatagcccg cggcatacac gccgaccgga tgcagcgcaa acagcgaggc
    17581 gcccaccatc gaccacgtcc gagccctgct ttcgtcgtct gcatcgcccg cggacacggt
    17641 gcgcagcaag ttgcagctca gcttgaagac catcaagctg cacgcgaggt ggaaggcgag
    17701 gctcacgagg cgatgagccg gcattccgcc gatggtgacc tcggtcagcg caagcgtgaa
    17761 ataagggagg agccgaagac cgaggcccag cggatgggtg gcgtagtaga aaaagtgctc
    17821 acccgagaag aacacccagt cgtcgaagac gcgcggattg ttgaggaagg gcaggtacag
    17881 cgcacaggcg agcgccgcga ccaccgaggc gcacgcccac acggggagcc gtccgatgtc
    17941 cgcgcggttc aagatggccg ttcgtggtcg aaaaaaaagg taccccgaag ggtacctttt
    18001 ttgcagcgag ggctggtgat cagccgcggc aggagccggg cagatacttg ccaatgacgg
    18061 tcgtgccgtc cgcgctcgcc ccgcagcgcc acccgaacag cgaggtcgca ccgacgtccg
    18121 actgtgcggc cgggacggtc ggggtgcgca gcggcagcag ggtgacgacc ttgttgtcca
    18181 ccgcggtgtt gccgatcccc tgcgtggtga cctgcacgag accgttgatc gtcgtgtcga
    18241 tggtcttcac gtacttcgag gcaccgttcg ggttgttggc gctcgtcact tcgcagcccc
    18301 agttgccggc ctggatcgaa gcgctgttgc cgctctggaa cacctccgtg atcgacgtac
    18361 ggcacgcgct cgcggccagg atcacctcgg acatcttcgc ccggacggtg tagtcctgat
    18421 acgcgggcag cgcgacggcc gccagaatgc cgatgatcgc gacgacgatc atcagctcga
    18481 tcagcgtaaa gcccttttgc atcttcttca tgagacctcc tggttcttgt cacgccctga
    18541 aaaaaggcgc cgggatcgga gcagcaagcg ccatgcccgt gccgcatttc gcttcaacat
    18601 attgattgta ttcatatttt attagacacg gattctgtag tgcgaccagg gtcggccgtg
    18661 ctagggcggg gattgacgct gtgcgtcacc acgggtaaac ccgaagtcga gccgagtcca
    18721 gatcagggcg cgtaccccgg aatctgcagc tcgtccaccg cgtcgatctg ctccttgtcg
    18781 aggaaggcct cggcgtaacg caaatagacc ttcctgcgaa tgaacacgtc gaatagatcg
    18841 ggatcgatgt gcccgccgag cgcgaacgtg ccgaggatct tcagcgactc cgacagcgtc
    18901 ttgccgcgct tgtacggacg gtccttcgcg gtgagcgcct cgaagatatc cgcgataccc
    18961 atcacgcggg cctgcaccga catctgctcg cgggtgagcc cgcgcgggta gcccttgccg
    19021 tccatgcgct cgtgatgccc gcccgcgtac tccggcacgt tcctcaagtg cttcggccat
    19081 ggaagcgcct cgagcatctt gatcgtcgcg acgatgtggt gattgatgat ctcgcgctcg
    19141 gcgccggtga gcgttccgcg cgcgatggtg aggttggtcg tctcgtcctc ggtcaggaaa
    19201 tcggcggtgt gacccgagac gtcggtccac ttgtatcccg tggcgatgcg gcgcacgcgg
    19261 tcctgcgccg ccggcgacat ctgctcgccg ccgatgttgc aatgcctcag gaagtcgcgg
    19321 tccgcgtcga tgtggcgcag cgactcgcgc agcgactggt cgatgtcgtt cagtcgggcg
    19381 cgcgcgtcgg ccccgcccgc ccggacg
//
LOCUS       DATDMW010000240         8367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_570597, whole genome shotgun sequence.
ACCESSION   DATDMW010000240 DATDMW010000000
VERSION     DATDMW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570597"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /gene="mdoH"
                     /locus_tag="VI319_10240"
     CDS             <1..1211
                     /gene="mdoH"
                     /locus_tag="VI319_10240"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEY6864269.1"
                     /translation="FIRHCALGRLPGSGSLSGEILSHDFVEAALMRRAGWAVWIVYDL
                     PGSYEEMPPNLIDELKRDRRWCQGNLINSRLFLASGLHPAHRAVFMTGVMAYLSAPLW
                     FLFLVLSTVLLAVHVLVPPTYFTQPGQLFPLWPEWHVGWAIALCSATALLLFLPKVLS
                     VLLLVGRRGFGGAARLVAGVAAEFVFSALLAPVRMLFHTRFVVGALTGWSVQWKSPSR
                     EDAETTWSEALRRHGAHTLLGLAWAALVYRLDPSFLWWLLPVVGALLLSIPVSVYTSR
                     VSLGKAARRAGLFVIPEELDPPEALREAHAAEAGPALPGFAEAVADPVVNALVCASGI
                     ARLRQPVAARRERDRLVERARRDGPEALLPRQKLMLLNDPLALSRLHFEASKVVPLRR
                     ADKALHADLR"
     gene            1242..2186
                     /locus_tag="VI319_10245"
     CDS             1242..2186
                     /locus_tag="VI319_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864270.1"
                     /translation="MKKRWTLAAAAAGFACAASAQQFPSHPIRWVVPFAPGGPTDINS
                     RILAPKLAERLGQPVLVENRVGAAGNIGTEAVAKAAPDGYTVLYAVPSVITNPFFFQG
                     SPDPKELAPVIQVANVPMVLLASTQFEPKSAADIVAAIKAKPGTVSCGSSGALPTVGC
                     ELLRSRSADMIMVLYKGNGPALNALMGGEINLLFDVVNTAGPQVRTGRVRAVATLSPK
                     RGIAAFPDLPTLSETLPGFEFVTWHGVMAPAGTPREIIERWNREIGAALELPDVRARL
                     TDTGFEIVGGGPEVFAERLARDQQLFGDILRKAGVKPQ"
     gene            2239..2314
                     /locus_tag="VI319_10250"
     tRNA            2239..2314
                     /locus_tag="VI319_10250"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2272..2274,aa:Lys,seq:ctt)
     gene            2321..3070
                     /locus_tag="VI319_10255"
     CDS             2321..3070
                     /locus_tag="VI319_10255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6864271.1"
                     /translation="MSSAQTLNCPVCGGACALHAEVDIGRSMTGRDDAAGDVSVAYWL
                     CGDCGFCFAPEMARWSRDQMLARVYNESYARHDPDSVELRPGENAKLLERLFGRHKGA
                     IRHLDYGGGAGELSRRLREAGWDSTSYDPFFDAGRELRELGKFSLVTAFEVVAHLPNV
                     NRLMEEIYGVLAPDGLVLFSTVVSDGHIARGMPLDWWYAAPRNGHISLHSRLSLTMMG
                     AKRGFRYAALSEVLHVYCRTVPPWAAAFVKE"
     gene            3320..5146
                     /locus_tag="VI319_10260"
     CDS             3320..5146
                     /locus_tag="VI319_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6864272.1"
                     /translation="MKPTDIAGPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRYDDAY
                     MVNWRSNVFPGILGRTCDRPCEPACRRGRVEEDNQEKAEPVAICRLKRVAADNKDKAG
                     VKAKMPKAAPKKNGKRVACIGAGPASLTVARDLAPLGYHVTVFDQDPRAGGMIWSQIP
                     RFRLPMEVIDEEVAYVLDLGVEFKGGVKIESMKKLLGQGYDAIFVGSGAPRGRDLDIP
                     GRKEAAKNIHIGIDWLSSVSFGHTTKIGKRVIVLGGGNTAMDCCRSSKRLGGEDVKVI
                     VRSGFEEMKASPWEKEDAQHEGIPILNYLVPKTFEHRNGKLVGMTFEKVKAVYDDKGK
                     RSLVPTGEPDQRFECDDVLVAVGQENAFPWIERDSGIEFDKWGLPVLDKQTMQSTLPN
                     VFFGGDSAFGPKNIIWAVAHGHDAAISIDKHCHGESLKERPSPMVNLVSQKMGIHEWS
                     YDNAISPDERYAVPWADKKVTLKNIKVEVELGFDPATGFKEAQRCLNCDVQTVFSDRL
                     CIECDACVDICPMDCITFTANGEEPELRARLNAPAPNLSQDLYVSGALKTGRVMVKDE
                     DVCLHCGLCAERCPTGAWDMQKFLLDLTHAGPSCRPYHKKAA"
     gene            5148..6992
                     /locus_tag="VI319_10265"
     CDS             5148..6992
                     /locus_tag="VI319_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY6864273.1"
                     /translation="MAKRIESVNAFVVKFANVNGSGSASANELFAKSILRMGVPVSPR
                     NIFPSNIQGLPTWYEVRVSESGWLGRRGGVDMMVAMNPQTWDADVKEIDAGGYLFYDN
                     TKPLPKSKFRDDINVIGMPLTEITNQAYSDPRQRQLFKNIIYVGALSALLDVDPGVIA
                     ELIGEQYKGKEALLKPNVTALKMGRDYALAHLPCPLGIRVKKADNVGDKIFVDGNSAA
                     ALGCVYGGATVCAWYPITPSSSLAEAFQRYCSRLRVDKDTGKNKFAIVQAEDELASIG
                     IVTGAGWNGARAFTATSGPGISLMSEFIGLAYFAEIPATIIDVQRGGPSTGMPTRTQQ
                     SDVLKCAYASHGDTKHVLLFPEDPHECFTMAAQCLDLADRLQTPVFLMTDLDIGMNQR
                     LCKPFAWDDSKVYDRGKVMTAEMLDSGKDFGRYLDVDGDGIPFRTYPGTHATKGGYFT
                     RGTSKNAYAIYSERGEDYKYNMQRLQKKFETAKTLVPKAVLTASKEPARFGAIYYGST
                     SPAMAEALDALARQGIFVNALRIRAFPFQDEIFDFVASHSKVFVIEQNRDAQMKTLLV
                     NDAGINPASLISILHYDGTPITARFITKEIAEIVSALNVRPIKKDKAA"
     gene            6992..7165
                     /locus_tag="VI319_10270"
     CDS             6992..7165
                     /locus_tag="VI319_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864274.1"
                     /translation="MGQGFRLGGDDDDDDEDENDKHRKDDLEDEDEEDEDEDDEDEDE
                     DDDDEDTLQVMSS"
     gene            7188..8243
                     /locus_tag="VI319_10275"
     CDS             7188..8243
                     /locus_tag="VI319_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY6864275.1"
                     /translation="MTYLAKPKLHHPTLPKNKVGYTRRDYEGKVSTLCAGCGHDSISA
                     ALIQAFFELDILPHKVAKLSGIGCSSKTPDYFLGNSHGFNTVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGLGQFAHVMRRGVNMTYILENNGVYGLTKGQFSATADKGSKA
                     KKGMVNADAPIDTISLALLMGATFVARSFSGDKKQLVPLVKAAIAHQGAAFIDVISPC
                     VAFNNHAGSTKSYDYVREHNEAVNRLDFWPNREAINIDYSEGGVIDVPQHDGSVIRLR
                     KTHPEYDPTDRIRAANQVAEHAAKGEVLTGLLYVDPNPEDLHAHLNTVEKAFNTLGEK
                     ELCPGSGVLEKVNAALR"
BASE COUNT         1451 a         2791 c         2848 g         1277 t
ORIGIN      
        1 ctttcatccg gcactgcgcg ctcgggcggc tgccgggcag cggctcgctc tcgggggaga
       61 tcctgtcgca cgacttcgtc gaggcggcgc tcatgcgccg cgccggctgg gcggtgtgga
      121 tcgtctacga cctccccggc agctacgagg agatgccgcc caacctgatc gacgagctga
      181 agcgcgaccg gcgctggtgc cagggcaacc tgatcaactc gcggctgttt ctcgcgagcg
      241 gactgcaccc ggcgcaccgc gccgtgttca tgaccggcgt gatggcgtac ctgtcggcgc
      301 cgctttggtt cctgttcctc gtgctctcga cggtgctgct cgcggtgcac gtgctcgtgc
      361 cgccgacgta cttcacgcag ccggggcagc tcttcccgct gtggcccgag tggcacgtcg
      421 gctgggcgat cgcgctgtgc agcgcgaccg cgctgctgct gttcctgccg aaggtgctga
      481 gcgtgctgct cctcgtcggg cgtcgcggct tcggcggcgc ggcgcgcctc gtcgccgggg
      541 tggcggccga gttcgtattc tcggcgctgc tcgcgccggt gcggatgctg ttccatacgc
      601 gcttcgtcgt cggcgcgctc accggctggt cggtgcagtg gaaatcgccg tcgcgcgagg
      661 acgccgagac cacctggagc gaggcgctgc gccggcacgg cgcccacacg ctgctcgggc
      721 tcgcgtgggc ggcgctggtc taccgcctcg atccgagctt cctgtggtgg ctgctgcccg
      781 tggtcggcgc gctgctgctc tcgatcccgg tgtcggtgta caccagccgc gtctcgctcg
      841 ggaaggccgc gcgccgcgcc ggcttgttcg tgatcccgga ggagctcgat ccgcccgagg
      901 cgctgcgcga ggcccacgcg gcggaggcgg gcccggcgct gccgggtttc gccgaggcgg
      961 tcgccgatcc ggtcgtgaac gcgctcgtct gcgcctcggg catcgcgcgg ctcaggcagc
     1021 ccgtggcggc gcggcgcgag cgcgaccggc tggtcgagcg cgcgcgccgc gacgggcccg
     1081 aggcgctgct cccgcggcag aagctgatgc tgctgaacga ccccctggcg ctgtcgcggc
     1141 tgcacttcga ggcgagcaag gtggtcccgc tgcggcgtgc ggacaaggcg cttcacgccg
     1201 acctgcgcta aagtcgcgca aagccgagag gagggacacc ggtgaagaag cgctggacgc
     1261 tggccgccgc ggcggcgggt ttcgcgtgcg ccgcgagcgc acaacaattt ccgtcgcacc
     1321 cgatccgctg ggtggtgccg ttcgcgcccg gcgggccgac cgacatcaac tcgcgcatcc
     1381 tcgcgccgaa gctcgccgag cggctcggcc agccggtgct ggtcgagaac cgcgtcggcg
     1441 ccgcgggcaa catcggcacc gaggcggtgg cgaaggcggc gcccgacggc tacacggtgc
     1501 tctacgcggt gccgtcggtg atcacgaacc cgttcttctt ccagggcagc ccggatccga
     1561 aggagctcgc gcccgtcatc caggtggcga acgtgccgat ggtgctgctc gcgagcaccc
     1621 agttcgagcc gaagtcggcc gccgacatcg tcgccgcgat caaggcgaag ccgggcacgg
     1681 tgagctgcgg ctcctcgggc gcgctgccga cggtgggctg cgagctgctg cgctcgagga
     1741 gcgccgacat gatcatggtg ctctacaagg gcaacggccc ggcgctgaac gcgctcatgg
     1801 gcggcgagat caacctgctc ttcgacgtcg tgaacaccgc ggggccgcag gtgcgcaccg
     1861 gccgcgtgcg cgcggtcgcg acgctctcgc cgaagcgcgg catcgcggcg tttcccgatt
     1921 tgccgaccct gtccgagacg ctgcccgggt tcgagttcgt cacctggcac ggcgtgatgg
     1981 cgccggccgg gacgccgcgc gagatcatcg agcgctggaa tcgcgagatc ggcgccgcgc
     2041 tcgagctgcc cgacgtgcgc gcgcgcctga ccgacaccgg cttcgagatc gtcgggggag
     2101 ggccggaggt cttcgccgag cgcctcgcgc gcgaccagca gctcttcggc gacatcctgc
     2161 ggaaggcggg cgtcaagccg cagtagcgaa tccttcgctg cgctcaggat gacgggacac
     2221 tgctaaaatc ggcctcccgg gtcgttagct cagctggtag agcagcggac tcttaatccg
     2281 ttggtcgaag gttcgatccc ttcacggccc accacaaacc ttgtcctcgg cccaaaccct
     2341 caactgcccg gtgtgcgggg gcgcctgcgc gctgcacgcc gaggtcgaca tcgggcgctc
     2401 gatgaccggg cgcgacgacg ccgccggcga cgtgtcggtc gcgtactggc tgtgcgggga
     2461 ctgcggcttc tgcttcgcgc ccgagatggc gcgctggagc cgcgatcaga tgctcgcgcg
     2521 cgtctacaac gagagctacg cgcgccacga cccggactcg gtagagctgc gcccgggcga
     2581 gaacgccaag ctgctcgagc ggctcttcgg gcgccacaag ggggcgatcc gtcacctgga
     2641 ctacggcggc ggcgccgggg agctctcgcg gcgcctgcgc gaggccggct gggattcgac
     2701 ttcctacgat ccgttcttcg acgccggccg ggagctgcgc gagctcggca agttcagcct
     2761 ggtgaccgcg ttcgaggtgg tcgcgcacct gcccaacgtc aatcggctga tggaggagat
     2821 ctacggcgtg ctcgcgccgg acgggctggt gctgttcagc accgtcgtct ccgacgggca
     2881 catcgcgcgc ggaatgccgc tcgactggtg gtacgccgcg ccgcgaaacg ggcacatcag
     2941 cctgcactcg cgcctgagcc tcaccatgat gggcgccaag cggggcttcc gctacgccgc
     3001 cctgtccgag gtgctgcacg tctattgccg gaccgtgccg ccctgggcgg cggcattcgt
     3061 caaagaataa acaataaata cagatttctg tagcattatt ctcaggaagt acatcaattc
     3121 aatcggcggt cctccggaga ggccgccggt gcgttgatcc aggtgcgatc tccggcgtcc
     3181 gcgccgcggc cgccgccatc caaaccgcgc ctacgatttt gataatcaag ctattcatca
     3241 ccaatccgcg ggcgtatcat tcgccccacg cggacgcgcc aacaagactc acaaaggcgt
     3301 ccctctggag aaagttgcgt tgaagccgac tgatattgct gggcccgact acttccacaa
     3361 agtggtcgat tgccagtggg cttgcccggc ccacaccccc gttcccgaat acatccgatt
     3421 gatcgcagca ggccggtacg acgacgcgta catggtcaac tggcgctcga acgtcttccc
     3481 cgggattctc ggccgcacct gcgatcgccc gtgcgagccg gcgtgccggc gcgggcgggt
     3541 ggaggaggac aaccaggaga aggccgagcc cgtcgcgatc tgccgtctca agcgcgtcgc
     3601 cgcggacaac aaggacaagg cgggcgtcaa ggcgaagatg ccgaaggcgg cgccgaagaa
     3661 gaacggcaag cgcgtcgcgt gcatcggcgc ggggccggct tcgctcacgg tcgcgcgcga
     3721 cctcgcgccg ctcggctacc acgtcaccgt gttcgaccag gacccgcgcg cgggcgggat
     3781 gatctggtcg cagatcccgc gcttccgcct gccgatggag gtgatcgacg aggaggtcgc
     3841 gtatgtcctc gacctgggcg tcgagttcaa gggcggcgtg aagatcgagt cgatgaagaa
     3901 gctgctgggc caaggctacg acgcgatctt cgtcggctcg ggcgcgccgc gcgggcgcga
     3961 cctcgacatc cccgggcgca aggaagccgc gaagaacatc cacatcggca tcgactggct
     4021 gtcgtcggtg tcgttcggcc acacgacgaa gatcggcaag cgcgtcatcg tgctgggcgg
     4081 cggcaacacg gcgatggact gctgccggtc gtccaagcgc ctcggcggcg aggacgtgaa
     4141 ggtgatcgtg cgctcgggct tcgaggagat gaaggcctcg ccctgggaaa aggaggacgc
     4201 gcagcacgag ggcatcccga tcctcaacta tctcgtgccg aagaccttcg agcacaggaa
     4261 cggcaagctc gtcgggatga ccttcgagaa ggtgaaggcg gtctacgacg acaagggcaa
     4321 gcgcagcctg gtcccgaccg gcgagcccga tcagcgcttc gagtgcgacg acgtgctcgt
     4381 cgcggtgggg caggagaacg ccttcccctg gatcgagcgc gactcgggca tcgagttcga
     4441 taagtggggg ctgccggtcc tcgacaagca gacgatgcag tcgacgctgc cgaacgtgtt
     4501 cttcggcggc gactcggcgt tcgggccgaa gaacatcatc tgggcggtgg cgcacggcca
     4561 cgacgccgcg atctcgatcg acaagcattg ccacggggag agcctgaagg agcgcccctc
     4621 gccgatggtg aacctcgtgt cgcagaagat gggcatccac gagtggagct acgacaacgc
     4681 gatctcgccc gacgagcgct acgcggtgcc gtgggcggac aagaaggtga cgctgaagaa
     4741 catcaaggtc gaggtcgagc tcggcttcga ccccgcgacc gggttcaagg aagcgcagag
     4801 gtgcctcaac tgcgacgtgc agacggtgtt cagcgaccgc ctgtgcatcg agtgcgacgc
     4861 ctgcgtcgac atctgcccga tggactgcat cacgttcacg gcgaacggcg aggaaccgga
     4921 actgcgcgcg cggctgaacg ccccggcgcc aaacctgtcc caggacttgt acgtgtccgg
     4981 ggctctcaag accgggcggg tcatggtgaa ggacgaggac gtctgcctgc actgcggcct
     5041 gtgcgccgag cgctgcccga cgggcgcctg ggacatgcag aagttcctcc tcgacctgac
     5101 ccatgccggc ccctcctgcc gcccctacca caagaaggcc gcctgacatg gccaagcgga
     5161 tcgagtcggt caacgcgttc gtggtcaagt tcgcgaacgt caacggctcg gggtcggcct
     5221 ccgcgaacga gctcttcgcc aagtccatcc tgcgcatggg cgtgccggtc agcccgcgca
     5281 acatcttccc gtcgaacatc caggggctgc cgacctggta cgaggtgagg gtgtcggagt
     5341 cggggtggct cgggcgccgc ggcggcgtcg acatgatggt ggcgatgaac ccgcagacct
     5401 gggacgccga cgtgaaggag atcgacgccg gcggctacct cttctacgac aacaccaagc
     5461 cgctgccgaa gtcgaagttc cgcgacgaca tcaacgtgat cgggatgccg ctcacggaga
     5521 tcacgaacca ggcgtactcg gatccgcgcc agagacaact cttcaagaac atcatctacg
     5581 tcggcgcgct ctcggcgctg ctcgacgtcg acccgggcgt gatcgccgag ctgatcgggg
     5641 agcagtacaa gggcaaggaa gcgctgctga agccgaacgt cacggcgctg aagatggggc
     5701 gcgactacgc gctcgcgcac ctgccgtgcc cgctcgggat ccgggtgaag aaggccgaca
     5761 acgtcggcga caagatcttc gtcgacggca actcggccgc ggcgctcggc tgcgtctacg
     5821 gcggggcgac ggtgtgcgcg tggtacccga tcacgccgtc gtcgtcgttg gccgaggcgt
     5881 tccagcgcta ctgctcgcgg ctgcgcgtcg acaaggacac gggcaagaac aagttcgcga
     5941 tcgtgcaggc ggaggacgag ctcgcctcga tcggcatcgt gacgggggcg gggtggaacg
     6001 gggcgagagc cttcaccgcg acctcggggc ccggcatctc gctcatgagc gagttcatcg
     6061 ggctcgcgta cttcgccgag atcccggcga cgatcatcga cgtgcagcgc ggcgggcctt
     6121 cgaccggcat gccgacgcgc acgcagcagt cggacgtgct gaagtgcgcc tacgcgtcgc
     6181 acggggatac gaagcacgtg ctgctcttcc ccgaggaccc gcacgagtgc ttcacgatgg
     6241 cggcgcagtg cctggacctc gccgaccggc tgcagacgcc ggtgttcctg atgaccgatc
     6301 tcgacatcgg catgaaccag cgcctttgca agccgttcgc ctgggacgac tccaaggtct
     6361 acgaccgcgg caaggtgatg acggccgaga tgctggacag cggcaaggac ttcggccgct
     6421 acctcgacgt cgacggcgac ggcatcccgt tccgcaccta cccggggacg cacgcgacga
     6481 agggcggcta cttcacgcgc ggcacgagca agaacgcgta cgcgatctac tcggagcgcg
     6541 gcgaggacta caagtacaac atgcagcgtc tgcagaagaa gttcgagacg gcgaagacgc
     6601 tggtgccgaa ggcggtgctg acggcgtcga aggagccggc gcgcttcggc gcgatctact
     6661 acggctcgac gagcccggcg atggccgagg cgctcgacgc attggcgcgc caggggatct
     6721 tcgtcaacgc gctgcgcatc cgcgccttcc cgttccagga cgagatcttc gacttcgtgg
     6781 cctcgcactc gaaggtgttc gtgatcgagc agaaccgcga cgcgcagatg aagacgctgc
     6841 tggtgaacga cgcggggatc aaccccgcga gcctgatctc gatcctgcac tacgacggca
     6901 cgccgatcac cgcgcgcttc atcaccaagg agatcgccga gatcgtgtcg gcgctgaacg
     6961 tgcgtccgat caagaaggac aaggcggcgt gatggggcag ggcttccgcc tcggcggcga
     7021 cgatgacgac gacgacgagg acgagaacga caagcaccgc aaggacgacc tcgaggacga
     7081 ggacgaagag gacgaggacg aggacgacga agacgaagac gaagatgacg acgacgagga
     7141 caccctccaa gtgatgtcgt cctgagcgaa gcgaaggacc tgcttttatg acctacctag
     7201 ccaaacccaa gctccaccac ccgacccttc ccaagaacaa ggtcgggtat acgcgtcggg
     7261 actacgaagg caaggtctcc accctctgcg ccggctgcgg ccacgactcg atctccgccg
     7321 cgctgatcca ggccttcttc gagctcgaca tcctgccgca caaggtggcg aagctctcgg
     7381 gcatcggctg ctcctccaaa acgccggact acttcctcgg caactcgcac ggcttcaaca
     7441 cggtgcacgg gcgcatgccc tccgtgctca cgggcgcgaa cctcgcgaac cgcgacctga
     7501 tttacctcgg cgtctcgggc gacggcgact cggcctcgat cggcctgggg cagttcgcgc
     7561 acgtgatgcg ccgcggcgtg aacatgacgt acatcctcga gaacaacggc gtctacggcc
     7621 tgacgaaggg ccagttctcc gcgaccgccg acaagggctc gaaggcgaag aagggcatgg
     7681 tcaacgccga cgcgccgatc gacaccatct cgctcgcgct gctgatgggc gccaccttcg
     7741 tcgcgaggag cttctccggc gacaagaagc agctcgtccc gctcgtcaag gccgcgatcg
     7801 cgcaccaggg cgccgcgttc atcgacgtga tctccccgtg cgtcgcgttc aacaaccacg
     7861 cgggatcaac gaagagctac gactacgtgc gcgagcacaa cgaggcggtg aaccgcctcg
     7921 acttctggcc caaccgcgag gccatcaata tcgactacag cgaaggcggc gtcatcgacg
     7981 tgccccagca cgacggcagc gtcatccgcc tgcgcaagac ccaccccgag tacgacccga
     8041 ccgaccgcat ccgcgccgcc aaccaggtcg ccgagcacgc cgccaaaggc gaagtgctca
     8101 ccggcctgct ctacgtcgac cccaaccccg aagacctcca cgcccacctc aacaccgtgg
     8161 agaaagcgtt caacacgctg ggagagaaag agctttgtcc ggggagtggg gtgttggaga
     8221 aggttaacgc ggcgttgcgg taggcttatc gaacgataag cgatcataca gcccatggct
     8281 gacgataacg ccggtctact gcggtactcc gttagcctcg taactcaggg caatcatcgg
     8341 ttctatacgc taacgatgcc gagcgat
//
LOCUS       DATDMW010000241        14681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1941274, whole genome shotgun sequence.
ACCESSION   DATDMW010000241 DATDMW010000000
VERSION     DATDMW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14681
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941274"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VI319_10280"
     CDS             complement(<1..1434)
                     /locus_tag="VI319_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6864276.1"
                     /translation="MGAMNYIGASVKRKEDYRFLTGAGSYTDDVALPHQTYATFVRSP
                     HAHAKIKSIKKDKALKAPGVIAIFTGDDLGAAKVNGLPCGWLITDVNGQPMKEPPHPC
                     LAQGKVRYVGDHVAVVIAETQQQARDAAELVQVDYDVLPAVVRADTARKKGAPAIHDI
                     APDNTCYVWGLGDKAAVDKAFAQAAHVTTLEFINNRLIPNAIEPRAANASYSRADDSY
                     TLYVASQNPHVERLLMTAFVLGLPEHKVRVVAPDVGGGFGSKIYLYAEDVVLTWASKQ
                     INRPVKWTAERSESFVSDAHGRDHVTKAELAMDKDGKFLAMRVTTTANLGAYLSTFAS
                     CIPTILYATLLAGQYTTPVIYCEVTAVFTNTAPVDAYRGAGRPEATYVVERLVETAAR
                     EMKLDPSEIRRRNFIKSFPYQTPVALLYDTGDYDATLRAALKLADVAGFPARRADAEK
                     RGKLRGLGYACYIEACGIAPSNVAGALG"
     gene            complement(1439..1912)
                     /locus_tag="VI319_10285"
     CDS             complement(1439..1912)
                     /locus_tag="VI319_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6864277.1"
                     /translation="MTVKVSMTVNGKPASAEVEERTLLVNFIRENLRLTGTHVGCDTA
                     QCGACTIHLNGRAVKSCNMLAMQAEGADILTIEGLAKADGTLHPMQEAFREHHGLQCG
                     FCTPGMVMSAIDLVKNFPNPTEEQIREQLDGNLCRCTGYHNIVKAIKAASQTMRA"
     gene            2113..3126
                     /locus_tag="VI319_10290"
     CDS             2113..3126
                     /locus_tag="VI319_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY6864278.1"
                     /translation="MDSVDLEVIRSAVKWTESGHRATLGTVTHTWGSAPRPVGAMLVI
                     RDDGQVMGSVSGGCVEDDLIDKVKSRAIAADRPEVTVYGVTQEQATRYGLPCGGTLQI
                     VLEPVSAESKLKELLAVIERHELTARTLDMRTGKVRMEPGKGSDVLEFDGKVLKTVHG
                     PSWRLLVIGAGQVTRYLAQMGQALDYHVTVCDPREEYAEGWDLKDVPIQRGYPDDVVL
                     ALNLDAHSAVVAVTHDPKLDDAALLEALKSPAFYVGALGSKKNNDARRKRLAEFDLAA
                     SEIERLRGPVGLKIGSKTPPEIAVAILAEMTAVRRGVQIPETIEPPKVNISAGCTIPQ
                     ETP"
     gene            3149..3730
                     /locus_tag="VI319_10295"
     CDS             3149..3730
                     /locus_tag="VI319_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY6864279.1"
                     /translation="MPAKIRGILLAAGYSKRFGSNKLLQALPAGGPDAGMPIALASAK
                     HLLEVLPDSIAVVRPRAQKLAALLRDAGCNTVVCKNAAEGMGTSLAAGVRSADDAGGW
                     VVALADMPFIRPETIRAVVSALESGAAIAALSYRGQRGHPVGFGRGFLEELSALAGDE
                     GAREVLSRHADALSLRDTDDPGVLRDIDRPSDL"
     gene            3731..5107
                     /locus_tag="VI319_10300"
     CDS             3731..5107
                     /locus_tag="VI319_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY6864280.1"
                     /translation="MDLDPVLLSRLQFFWVVAFHILLPAFTVGLASYIAVLEGLHFFT
                     GRAVFLSTSRYWIRIFAVSFGMGVVSGIVMPFQFGTNWSRYSDATANVVGPLMAYEGL
                     TAFFLEAGFLGVLLFGRKLVPAWMHFFAALMVALGTLFSTFWILAANSWMQTPAGHTM
                     ADGRFFPQSWLEIIFNPSFPYRLAHNVVAFYITTAFVVIGAAAYLLRHGRAVEESRVM
                     QKMGFGLLILLVPLQVLLGDQHGLNTLEHQPAKVAAIEAHWRTEAHMPLLLFAWPDEQ
                     AEANRFEIAVPEAGSLILTHRRDGVVRGLKEFPREDRPPVAIPFFAFRVMVAIGLLML
                     AVVVSGAVLWARGRLHEATWFQSLCMACMPLGFIAVVAGWITTEVGRQPWVVYGLMRT
                     RDAVSPSLTGTDVLISLVLYALVYFFIFGAGLMFMARLVRAGPGAAGPAEAKDLQRPA
                     RPFSGAEA"
     gene            5104..6114
                     /gene="cydB"
                     /locus_tag="VI319_10305"
     CDS             5104..6114
                     /gene="cydB"
                     /locus_tag="VI319_10305"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY6864281.1"
                     /translation="MSLDLVPLWAAILALAVFMYVLLDGFDLGVGILARHAPDDPARD
                     LMMNSVAPIWDGNETWLVMGGIALLSAFPLAFAILLPALYFPLLLMLLGLIFRGVAFE
                     FRVMPGTRKRVWGHAFHWGSVVATFAQGVVLGNFVQGIPVEGRHFAGGSFDWIRPFPL
                     LVGLGLIAGYGLLGACWLVLKTEGELQRWARRRARGLLFGVLAFIAMVSVWTPLIQPR
                     VFERWFSWPNIAWFSPVPLITGATAWWLWRSLERRDLAPFLAAMGLFVLCYLGLAISI
                     WPMVIPYSVSLWDAAAAPGSQAFLALGTVFLLPIITMYTAWSYWVFRGKVRADTGYHS
                     AH"
     gene            complement(6111..6518)
                     /locus_tag="VI319_10310"
     CDS             complement(6111..6518)
                     /locus_tag="VI319_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEY6864282.1"
                     /translation="MPLVRIALMKGQPAAGRRIGAIVYRALLETIGVPVADNFQVITE
                     HDPETLVYDPGYLGIRRTDGFVAIQITLTEGRTVEAKRRLYATIVQNLRRELGVRPED
                     VFINLVEVKKENWSFGNGVAQYVQPAEALAERA"
     gene            complement(6529..6867)
                     /locus_tag="VI319_10315"
     CDS             complement(6529..6867)
                     /locus_tag="VI319_10315"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEY6864283.1"
                     /translation="MDLKVSRAGLLLEPHQVFGLSDAIGAQLQVRRGTVWITQHADGR
                     DVMVSQGGGFTLDRPGLAIVHALEPAEVAVAEPGAATHRRWWGSAKRWIISTFGPAAI
                     DRADPSRRYL"
     gene            6965..7912
                     /gene="gcvA"
                     /locus_tag="VI319_10320"
     CDS             6965..7912
                     /gene="gcvA"
                     /locus_tag="VI319_10320"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEY6864284.1"
                     /translation="MHKGLPSLDLLKGFEAAARNLSFTKAGAELFLTQSAISRQVKAL
                     EDELGVPLFRRKHRELLLTEAGQTLYKAVGEALHTLREAAAKLSARAGGMLTVTTTIS
                     FASLWLVPRLAEFRRQHPEIDVRIAATNDIVDLERDGIDVAIRYCLPEVAGAGAIELF
                     GEKAFPVCAPALVSSGRLSSPQDLSRHVLLHYDDPERRSPWLSWEVWFELVKAHGVRP
                     RGTLRFSHYDQMIAAALDGQGIALGRSPLVDRWVKDGRLVLPFGGRFISSKGTARAYF
                     ILAAPAAGNRPEVERFSRWVRAEALAAAAATAKSARSAA"
     gene            7923..8900
                     /locus_tag="VI319_10325"
     CDS             7923..8900
                     /locus_tag="VI319_10325"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEY6864285.1"
                     /translation="MNAPDHPLAVSWEDVASAAECLAGHAHRTPVMTSRTVDARTGAR
                     VFFKCENLQRIGAFKFRGAYNAVSRLSPEEKKRGILAYSSGNHAQAVALAGRELGVRV
                     VIVMPQDAPAVKIEATRGYGAEVVLYDKNKEIREEVAERTARERGLAMIPPFDHPHVI
                     AGQGTAAKELIEDAGALDWLFVPTSGGGLISGCAIAAARWSPGCKVVGVEPEAGDDAT
                     RSFRTKTLQSCHNPETIADGARTHALGKLTFPLVLRYVHDMATVSDAELARAMFYLWE
                     RMKIVVEPTGALGAAGLFERGLPVAGKKVGVILSGGNVDLKLAAKLITL"
     gene            complement(8890..10086)
                     /locus_tag="VI319_10330"
     CDS             complement(8890..10086)
                     /locus_tag="VI319_10330"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY6864286.1"
                     /translation="MPVRQTLATGAVPVQVFTDEVEASARRQLENVSKLPIVFHHVAA
                     MPDVHAGIGATVGSVIPTRGAIIPAAVGVDIGCGMSAVRLSLRAEQLPDSLSKLRSAI
                     ERAVPVGFDEHRDADALHDAAAPLEPRLARILERHPAIVSRKRDGRTRWVRQMGSLGG
                     GNHFIEVCLDESQRVWVMLHSGSRGLGNAIGQYFIAAAREEMTGLDARLPDRDLAWFG
                     EGSQLFDDYIEAVGWAQDYARANRDAMMRLVLDAIASRLPAFEVTGDAINCHHNYVER
                     ERHFGERVWVTRKGAIRAREGELGIIPGSMGARSYIVRGKGNAESFCSCAHGAGRRMS
                     RAEAKKRFTRADLEAQTSGVECRKDTGVLDEIPGAYKDIDKVMADQTDLVDVVHTLKQ
                     VVCVKG"
     gene            10140..11417
                     /gene="glyA"
                     /locus_tag="VI319_10335"
     CDS             10140..11417
                     /gene="glyA"
                     /locus_tag="VI319_10335"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY6864287.1"
                     /translation="MFPNKPLQQVDPDLWSAIQRENQRQEDHVELIASENYVSPAVLA
                     AQGSQLTNKYAEGYPGKRYYGGCEFVDVAEQLAIDRAKQLFRCEYANVQPHSGSQANQ
                     AVYMSVLQPGDTILGMALASGGHLTHGAAVNLSGRLYKAVTYGLDANEVIDYAQVEAL
                     ARQHKPRMIVAGASAYALAIDWKRLRAICDQVGATLFADVAHYAGLIVTGLYPSPIGI
                     ADFVTSTTHKTLRGPRGGLILAGAEHEKALNSSIFPNLQGGPLMHVIAAKAVAFKEAL
                     EPDFRDYQVQVMTNAKVMAKTLQSRGVRIVSGRTESHLFLVDLRAKNVTGKDAESALG
                     RAHITVNKNAIPNDPQKPMVTSGIRIGSPAMTTRGFRDAEAERLAHLIADVLDAPQDD
                     RVLKRVAGEVQELCRKFPVYRPAEAGAQRVAVA"
     gene            11440..11922
                     /gene="nrdR"
                     /locus_tag="VI319_10340"
     CDS             11440..11922
                     /gene="nrdR"
                     /locus_tag="VI319_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY6864288.1"
                     /translation="MKCPYCKSENTGVIDSRLSDDGESVRRRRQCEDCDKRFTTYERT
                     ELSMPMIVKSNGNRTAYDREKIRTGFMRALHKRPVPTEYVDQAINRVEQRIMALGERE
                     VASRVIGEMVMRELKKMDDVAYIRFASVYEDFQRVDDFRDAIQEVKRPGKAKARRAPK
                     "
     gene            11922..13013
                     /gene="ribD"
                     /locus_tag="VI319_10345"
     CDS             11922..13013
                     /gene="ribD"
                     /locus_tag="VI319_10345"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY6864289.1"
                     /translation="MADAADLGFMGRALELARLGLYTTTPNPRVGCVAVKDGDVVGEG
                     WHAKAGLPHAEVLALAQAGARGRGATLYLNLEPCSHHGRTPPCADALIAAGVARVVAA
                     MQDPNPQVAGAGFARLRAAGIRVEHGLMEEEARELNIGFVSRMTRGCPWVRMKIAASL
                     DGRTALANGKSQWITGDAARLDGHAWRARACAILTGIGTVKDDDPRLTVRGVDTPRQP
                     LKVLIDSRLEVDPAARLFEQGAVLVVAAREDRAKEEALRARGAEVICIPNAQGKTELA
                     SVMRELGRRGINELHVEAGTRLNGSLLREGCVDELLVYLAPSVMGDTGRGMFALPEPA
                     ALDRLVRLEMRGAERIGDDLRILARVRRD"
     gene            complement(13000..13944)
                     /locus_tag="VI319_10350"
     CDS             complement(13000..13944)
                     /locus_tag="VI319_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020830907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY6864290.1"
                     /translation="MSDPSTHPLPAAVIGVASGLGARDPRCRDGPAALERDGLLARLA
                     GRGVQLSWRSTLEPEHHPGQSRMRAISEICNRLAREVGATIRAGGLPVVVGGDHSCAI
                     GTWSGAARAVSPRGPLGLVWIDAHMDSHTPATSHSGMPHGMPLAALLGHGASRELAIE
                     VAEILHEGQIAPRHVCLVGVRSHEPEEEALLTRLGVRIVHMDELRARGLDAVMREAID
                     IARADTAGYGITLDLDAMDPLDVPGVGTPVRGGIAAPALIEALATCCAKDAALVALEV
                     VEYNPHRDVDRRTAQVVEDVIAAVLGPRPAAEASFSPA"
     gene            14014..14586
                     /locus_tag="VI319_10355"
                     /pseudo
     CDS             14014..14586
                     /locus_tag="VI319_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
BASE COUNT         2117 a         5026 c         5220 g         2318 t
ORIGIN      
        1 gccgagcgcg cccgccacgt tcgacggcgc gatgccgcag gcctcgatgt agcacgcgta
       61 cccgagaccg cgcagcttgc cgcgcttctc ggcgtccgca cgccgcgccg ggaagccggc
      121 gacgtcggcc agcttcagcg ccgcccgcag cgtcgcatcg tagtcgcccg tgtcgtagag
      181 cagcgcgacc ggggtctggt acgggaagct cttgatgaaa ttgcgccggc ggatctcgga
      241 gggatcgagc ttcatctcgc gcgccgcggt ctcgaccagg cgctcgacga cgtaggtcgc
      301 ctccgggcgt ccggcgccgc ggtaggcgtc gaccggcgcg gtgttggtga acaccgcggt
      361 cacctcgcag tagatcacgg gcgtcgtgta ctgcccggcg agcagcgtcg cgtacaggat
      421 ggtggggatg cacgaggcga acgtcgacag gtacgcaccc aggttcgccg tcgtcgtcac
      481 gcgcatggcg aggaacttgc cgtccttgtc catcgcgagc tcggccttcg tcacgtggtc
      541 gcggccgtgc gcgtcggaga cgaaggactc gctgcgctcg gcggtccact tcaccgggcg
      601 gttgatctgc ttggacgccc atgtcagcac gacgtcttcg gcgtagagat agatcttcga
      661 gccgaagccg ccgccgacgt cgggcgcgac cacgcgcacc ttgtgctcgg gcagcccgag
      721 cacgaacgcg gtcatcagca gccgctcgac gtgcgggttc tggctggcga cgtagagcgt
      781 gtagctgtcg tcggcgcgcg agtacgatgc gttcgccgcc cgcggctcga tcgcgttcgg
      841 gatgagccgg ttgttgatga actccagcgt cgtgacgtgc gcggcctgcg cgaacgcctt
      901 gtccaccgcc gccttgtcgc ccaagcccca gacgtagcag gtgttgtcgg gggcgatgtc
      961 gtggatcgcg ggagcgcctt ttttgcgcgc ggtgtcggcg cgcaccacgg ccggcagaac
     1021 gtcgtagtcg acctgcacca gctcggccgc gtcgcgcgcc tgctgctgcg tctcggcgat
     1081 cacgaccgcg acgtggtcgc cgacgtagcg cacttttcct tgcgcgaggc acgggtgcgg
     1141 cggctccttc atcggctggc cgttgacgtc cgtgatcagc cagccgcacg gcagcccgtt
     1201 caccttcgcc gcaccgagat cgtcgcccgt gaagatcgcg atcacgccgg gcgccttcag
     1261 cgccttgtcc ttcttgatcg acttgatctt cgcgtgcgcg tgcggggagc gcacgaacgt
     1321 ggcgtaggtc tggtgcggca gcgccacgtc gtcggtgtag ctgcccgcgc ccgtcagaaa
     1381 acggtagtcc tctttgcgct tcaccgaagc gccgatgtag ttcatcgcgc ccatggttct
     1441 aggccctcat cgtctgtgaa gcggctttga tcgccttcac gatgttgtga taacccgtgc
     1501 agcggcacag gtttccgtcg agctgctcgc ggatctgctc ctcggtcggg ttcgggaagt
     1561 tcttcaccag gtcgatggcg ctcatgacca tgccgggcgt gcagaagccg cactgcaggc
     1621 cgtggtgctc gcggaacgcc tcctgcatcg ggtgcagcgt gccgtcggcc ttggcgaggc
     1681 cctcgatcgt gaggatgtcg gcgccctcgg cctgcatcgc cagcatgttg cacgacttca
     1741 cggcgcgtcc gttcaggtgg atggtgcagg cgccgcactg cgcggtgtcg cagccgacgt
     1801 gggtgccggt caaacgcagg ttctcgcgga tgaagttgac gagcagcgtg cgctcctcga
     1861 cttctgcgct ggcgggtttg ccgttgacgg tcatggagac cttgacggtc atgggggagc
     1921 tcctttgcgg gaattttgcg gcgatgggga tcgtcaaaat gctagaccaa agcagccacg
     1981 gcaggcaact cggcggcttg aacgccgctc ggcttgaccc catctgatcg ggggtctagg
     2041 ctatcgccga taatcgagtg taagcattcg gtaatgtagt ttcgcccagg ttcgcacagc
     2101 gagaggaatc ccgtggacag cgtcgatctc gaagtcatcc gcagcgcggt gaagtggacc
     2161 gagtcgggcc accgggcgac gctcggcacc gttacgcaca cgtggggctc ggccccacgc
     2221 ccggtgggcg cgatgctggt catccgcgac gacggccagg tgatgggctc ggtctcgggc
     2281 ggctgcgtcg aggacgacct gatcgacaag gtgaagagcc gcgcgatcgc ggccgacagg
     2341 cccgaggtca ctgtctacgg cgtcacgcag gagcaggcga cgcgctacgg gctgccgtgc
     2401 ggcggcacgc tgcagatcgt gctcgagccg gtgagcgcgg agtcgaagct gaaggagctg
     2461 ctcgccgtga tcgagcggca cgagctcacc gcgcgcacgc tcgacatgcg caccggcaag
     2521 gtgcgcatgg agcccggcaa ggggtccgac gtgctcgagt tcgacggcaa ggtgctgaag
     2581 acggtgcacg ggccgagctg gcggctgctg gtgatcggcg ccgggcaggt gacgcgctac
     2641 ctcgcgcaga tggggcaggc gctcgactac cacgtcaccg tgtgcgaccc gcgcgaggag
     2701 tacgcggaag gctgggacct gaaggacgtt ccgatccagc gcggctatcc cgacgacgtc
     2761 gtgctcgcgc tgaacctcga cgcgcacagc gcggtcgtcg ccgtgacgca cgatcccaag
     2821 ctcgacgacg ctgcgctgct cgaggcgctc aagtcgccag cgttctacgt cggcgcgctc
     2881 ggctcgaaga agaacaacga cgcgcgccgc aagcgcctcg ccgagttcga cctcgcggcc
     2941 tccgagatcg agcggctgcg cgggccggtc ggcctcaaga tcggctcgaa gacgccgccc
     3001 gagatcgcgg tggcgatcct cgcggagatg acggccgtgc gccgcggcgt ccagatcccc
     3061 gagacgatcg agccgccgaa ggtcaacatc tcggccggct gcacgatccc gcaggaaaca
     3121 ccgtaagccg ccggttcacg gaggcgccgt gcccgcaaag atacgcggca tactgctcgc
     3181 ggcggggtat tccaagcgct tcggatcgaa caagctgctg caggccctgc cggcgggcgg
     3241 gcccgacgcc gggatgccga tcgcgctcgc ttcggccaag cacctgctcg aggttctgcc
     3301 cgattccatc gccgtcgtga ggccgcgcgc gcagaagctc gccgccctcc tgcgcgatgc
     3361 cggctgcaac acggtcgtgt gcaagaacgc ggccgagggc atggggacga gcctcgccgc
     3421 cggggtgcgc tccgccgacg acgcgggcgg ctgggtggtc gcgctcgccg acatgccgtt
     3481 catccgtccc gagacgatcc gcgcggtggt gagcgcgctc gaatcgggcg cggcgatcgc
     3541 ggcgctgtcg taccgcgggc agcgcgggca cccggtaggc ttcggccggg gctttctcga
     3601 ggagctgtcg gcgctggccg gcgacgaggg cgcgcgcgag gtcctttcgc ggcacgccga
     3661 cgcgctgtcg ctgcgcgaca ccgacgaccc tggcgtgctg cgcgacatcg accggccgag
     3721 cgacctctag atggacctcg atcccgtcct cctctcgcgc ctgcagttct tctgggtcgt
     3781 cgcgttccat atcctgctgc ccgcgttcac cgtcgggctc gcctcctaca tcgcggtgct
     3841 cgaggggctg catttcttca ccgggcgggc ggtgttcctc agcacctcgc gctactggat
     3901 caggatcttc gccgtgtcgt tcggcatggg cgtcgtgtcg ggcatcgtca tgccgttcca
     3961 gttcggaacc aactggagcc gctactcgga cgcgaccgcg aacgtggtcg ggccgctgat
     4021 ggcgtacgag gggctgaccg cgttcttcct cgaggcggga ttcctcggcg tgctgctctt
     4081 cgggcgcaag ctcgtgcccg cgtggatgca cttcttcgcg gcgctcatgg tcgcgctcgg
     4141 gacgctgttc tcgaccttct ggatcctggc ggcgaacagc tggatgcaga cgcccgcggg
     4201 acacacgatg gccgacggcc gcttctttcc gcagagctgg ctcgagatca tcttcaaccc
     4261 gtcgttcccg taccgcctcg cgcacaacgt cgtggctttc tacatcacga cggcgttcgt
     4321 cgtgatcggc gcagcggcgt acctgctgcg ccacgggcgc gcggtcgagg aaagccgggt
     4381 catgcagaag atgggcttcg gcctgctgat cctgctggtg ccgctgcagg tgctgctcgg
     4441 cgaccagcac gggctcaaca cgctcgagca ccagccggcg aaggtcgcgg cgatcgaggc
     4501 gcactggcgg accgaagcgc acatgccgct cctgctgttc gcatggcccg acgagcaggc
     4561 cgaggcgaac cgcttcgaga tcgcggtgcc cgaggcgggc agcctgatcc tcacgcatcg
     4621 ccgcgacggc gtggtgcgcg ggctgaagga gtttccgcgc gaggaccgtc cgccggtcgc
     4681 gattccgttc ttcgccttcc gcgtgatggt ggcgatcggc ctgctgatgc tcgccgtcgt
     4741 cgtcagcggc gcggtgctgt gggcgcgcgg gcggctgcac gaggcgacct ggttccagtc
     4801 gctgtgcatg gcgtgcatgc cgctcggctt catcgccgtc gtcgccggct ggatcacgac
     4861 cgaggtcggg cgccagccgt gggtcgtcta cgggttgatg cgcacgcgcg acgcggtgtc
     4921 gccgtcgctg accgggaccg acgtgctgat ctctctcgtg ctgtacgcgc tcgtctactt
     4981 cttcatcttc ggcgccgggc tgatgttcat ggcgcgcctg gtgcgcgccg ggcccggcgc
     5041 ggccggcccc gcggaggcga aggatctgca gcgcccggcg cggccgttct cgggggcgga
     5101 agcgtgagcc tcgatctcgt gccgctctgg gcggcgatcc tcgcgctcgc ggtcttcatg
     5161 tacgtgctgc tcgacggctt cgacctcggg gtcggcatcc tcgcgcgcca cgcgcccgac
     5221 gaccctgcgc gcgacctcat gatgaactcg gtcgcgccca tctgggacgg caacgagacc
     5281 tggctggtga tgggcgggat cgcgctgctc tcggcgttcc cgctcgcctt cgcgatcctg
     5341 ctgccggcgc tgtacttccc gctgctgctc atgctgctcg ggttgatctt ccgcggcgtc
     5401 gcgttcgagt tccgcgtcat gcccgggacg cgcaagcgcg tatgggggca cgcgttccac
     5461 tggggctcgg tggtcgcgac tttcgcgcag ggcgtcgtgc tcgggaattt cgtgcagggt
     5521 ataccggtgg aagggcgcca tttcgcgggc ggcagcttcg actggatccg accgtttccg
     5581 ctgctcgttg gcctgggcct catcgcgggc tacggcctgc tcggcgcgtg ctggctggtg
     5641 ctgaagaccg agggcgagct gcagcgatgg gcacggcggc gggcgagggg gctgctcttc
     5701 ggagtgcttg cgttcatcgc gatggtgagc gtgtggacgc cgctcataca accgcgggtg
     5761 ttcgagcgct ggttctcgtg gccgaacatc gcctggttct cgccggtgcc gctgatcacg
     5821 ggggccacgg cgtggtggct gtggcgatcg ctggagcggc gcgatctcgc gccgttcctc
     5881 gcggcgatgg gacttttcgt gctgtgctac ctgggcctcg cgatcagcat ctggccgatg
     5941 gtgatcccgt actcggtgag cctgtgggac gcggccgcgg ccccggggtc gcaggcgttc
     6001 ctcgcgctcg ggacggtgtt cctcctgccg atcatcacca tgtatacggc gtggtcgtac
     6061 tgggtgttcc gcggcaaggt gcgcgccgac accgggtacc actcggcgca ctaggcgcgc
     6121 tcggcgagcg cctccgcggg ctgcacgtac tgcgccacgc cgttcccgaa ggaccagttc
     6181 tccttcttca cctcgaccag gttgatgaac acgtcctcgg gacgcacgcc cagctcgcgc
     6241 cgcaggttct ggacgatggt cgcgtagagc ctgcgtttcg cctcgaccgt gcggccctcc
     6301 gtgagggtaa tctggatggc gacgaagccg tcggtccgtc ggatcccgag atagccgggg
     6361 tcgtagacga gcgtttccgg gtcgtgctcc gtgatgacct gaaaattgtc ggcgacgggg
     6421 acgccgatcg tctcgagcaa ggcacggtag acgatcgctc cgatgcgacg gccggcggcc
     6481 ggctgccctt tcatgagcgc gatgcgaacg agcggcatgg cagtcccctc agaggtagcg
     6541 tcgcgacggg tcggcgcggt cgatcgccgc gggtccgaac gtcgagatga tccagcgctt
     6601 cgcgcttccc caccaccgcc ggtgcgtcgc cgcgccgggc tcggcgaccg ccacctcggc
     6661 cggctcgagc gcgtgcacga tcgcgaggcc gggacggtcg agcgtgaagc ccccgccctg
     6721 cgagaccatc acgtcgcggc cgtcggcgtg ctgcgtgatc cacaccgtgc cgcgccgcac
     6781 ctgcagctgc gcgccaatcg cgtccgaaag cccgaacacc tggtgcggct ccagcagcag
     6841 cccggcgcgg ctcaccttga gatccatggc aactcctttc ataatgcgcg agacgcatgc
     6901 attattggcc cgcgtccata gcggcgcaaa cgacgttttt gcatggatca catgaggcat
     6961 gggaatgcat aaaggactgc cgtcgctgga tctgctgaag ggcttcgagg ccgccgcgcg
     7021 caacctgagc ttcaccaagg cgggcgccga gctgttcctc acgcagtcgg cgatcagccg
     7081 ccaggtgaag gcgctcgagg acgagctggg cgtgccgctg ttccgccgca agcaccgcga
     7141 gctgctgctg accgaggcgg gccagacgct gtacaaggcc gtgggcgagg cgctgcacac
     7201 gctgcgcgag gcggcggcga agctctcggc gcgcgcgggc ggcatgctca cggtgaccac
     7261 cacgatctcg ttcgcgtcgc tgtggctggt gccgcgcctc gccgaattcc gccggcagca
     7321 tcccgagatc gacgtgcgca tcgccgcgac caacgacatc gtcgatctcg agcgcgacgg
     7381 catcgacgtc gcgatccgtt attgcctgcc cgaggtcgcg ggcgcgggcg cgatcgagct
     7441 gttcggcgag aaggcgttcc cggtgtgcgc gcccgcgctc gtgtccagcg gccggctttc
     7501 gtcgccgcag gacctgtcgc gccacgtgct gctgcactat gacgatcccg agcgccgctc
     7561 gccctggctg agctgggagg tatggttcga gctggtgaag gcccacggcg tgcggcccag
     7621 gggcaccctg cgcttctcgc actacgacca gatgatcgcg gcggcgctcg acggacaggg
     7681 gatcgcgctc ggccgcagcc cgctcgtcga tcgctgggtg aaggacggcc ggctggtgct
     7741 gcctttcggc ggccggttca tatcgtcgaa gggcaccgcg cgcgcgtatt tcatcctcgc
     7801 cgcgccggcc gcgggaaacc ggccggaggt cgagcgcttt tcccgctggg tgcgcgccga
     7861 ggcgcttgcc gcggcggcgg cgaccgcgaa gtccgcgcgc agtgcggcgt agaatcggga
     7921 cgatgaacgc ccccgatcat ccgctcgccg tcagctggga agacgtcgcc tcggcggcgg
     7981 agtgcctcgc cggccacgcg caccgcacgc ccgtgatgac gtcgcgaacg gtcgacgcgc
     8041 gcacgggcgc gcgcgtgttc ttcaaatgcg agaacctgca gcgcatcggc gcattcaagt
     8101 tccgcggcgc ctacaacgcg gtgtcgcggc tgtcccccga ggagaagaag cgcggcatcc
     8161 tcgcgtactc gtcgggcaat cacgcgcagg cggtggcgct cgcgggccgc gagctcggcg
     8221 tgcgcgtcgt catcgtcatg ccgcaggacg ccccggcggt gaagatcgag gccactcgcg
     8281 gctacggcgc cgaagtggtg ctctacgaca agaacaagga gatccgggag gaggtcgccg
     8341 agcgcaccgc gcgcgagcgc gggctcgcca tgatcccgcc gttcgaccac ccgcacgtca
     8401 tcgccggcca gggcacggcc gcgaaggagc tgatcgagga cgccggcgcg ctcgactggc
     8461 tgttcgtgcc gacctcgggc ggcggcctca tctcgggctg cgcgatcgcc gcggcgcgct
     8521 ggtcgccggg ctgcaaggtg gtcggcgtcg agcccgaggc gggcgacgac gcgacgcgct
     8581 cgttccggac gaagacgctg cagagctgcc acaacccgga gacgatcgcc gacggcgcgc
     8641 gcacgcacgc gctcgggaag ctgacgttcc cgctggtcct ccgctatgtg cacgacatgg
     8701 cgacggtcag cgacgccgag ctcgcgcgcg cgatgttcta cttgtgggag cgcatgaaga
     8761 tcgtggtcga gccgacgggc gcgctcggcg ccgcgggcct gttcgagcgc gggctgccgg
     8821 tcgcggggaa gaaagtcggc gtgatccttt ccggcggcaa tgtcgatctc aagctggcgg
     8881 cgaagctgat caccctttga cgcacaccac ctgcttgagc gtgtgcacga cgtcgaccag
     8941 gtcggtctgg tcggccatga ccttgtcgat gtccttgtag gcgcccggga tctcgtcgag
     9001 gacgccggta tccttgcggc attcgacgcc gctcgtctgc gcctcgaggt cggcgcgcgt
     9061 gaatcgcttc ttcgcttcgg cgcggctcat gcgccggccg gcgccgtgcg cgcacgagca
     9121 gaaggactcc gcgttcccct tgccgcgcac gatgtaggag cgcgcgccca tgctgcccgg
     9181 gatgatgccg agctcgccct cgcgcgcgcg gatcgcgccc ttgcgcgtga cccagacgcg
     9241 ctcgccgaag tggcgttccc gctcgacgta gttgtggtgg cagttgatcg cgtcgccggt
     9301 gacctcgaag gccggcaggc gggacgcgat cgcgtcgagc accaggcgca tcatcgcgtc
     9361 gcggttggcg cgcgcgtaat cctgcgccca gccgaccgct tcgatgtagt cgtcgaagag
     9421 ctgtgagccc tcgccgaacc aggcgagatc gcggtccgga aggcgcgcgt cgaggcccgt
     9481 catctcctcg cgcgcggccg cgatgaaata ctgcccgatc gcgttgccga gcccgcggct
     9541 tcccgagtgc agcatcaccc agacccgctg cgactcgtcg aggcacacct cgatgaagtg
     9601 gttgccgccg ccgagcgagc ccatctgccg cacccatctc gtgcggccgt cgcgcttccg
     9661 gctcacgatc gccgggtggc gctcgaggat gcgcgcgagg cgcggctcga gcggcgccgc
     9721 ggcgtcgtgg agcgcgtcgg cgtcgcgatg ctcgtcgaag ccgacgggaa cggcgcgctc
     9781 gatggcggag cgcagcttcg agaggctgtc gggcagctgc tcggcgcgta gcgagagccg
     9841 gacggcgctc atcccgcagc cgatgtcgac gcccaccgcc gcggggatga tcgcgccacg
     9901 cgtcgggatc accgagccga cggtcgcgcc gatgccggcg tgcacgtcgg gcatggcggc
     9961 gacgtgatgg aacacgatcg gcagcttcga gacgttctcg agctggcgcc gcgcgctcgc
    10021 ctccacctcg tcggtgaaca cctgcaccgg caccgcgccc gtcgctaggg tttgtctgac
    10081 cggcatgatt cgcccgtggg cccaatgata agataccgtt ttgaccccgc cgtaacacca
    10141 tgttcccgaa caagcctttg cagcaggtcg atcccgacct ctggtccgcc atccagcgcg
    10201 agaaccagcg ccaggaggac cacgtcgagc tgatcgcctc cgagaactac gtcagccccg
    10261 cggtgctcgc cgcgcagggc tcgcagctca ccaacaaata cgccgagggc tatcccggga
    10321 agcgctacta cggcggctgc gagttcgtcg acgtcgccga gcagctcgcc atcgaccgcg
    10381 cgaagcagct gttccgctgc gagtacgcga acgtgcagcc gcactcggga tcgcaggcga
    10441 accaggcggt gtacatgtcg gtgctgcaac cgggcgatac catcctcggc atggcgctcg
    10501 cgagcggcgg gcacctcacg cacggcgcgg cggtcaatct gagcggccgg ctgtacaagg
    10561 ccgtgaccta cgggctcgat gcgaacgagg tgatcgacta tgcgcaggtc gaggcgctcg
    10621 cgcgccagca caagccgcgc atgatcgtcg ccggcgcgtc cgcatacgcg ctcgcgatcg
    10681 actggaagcg gctgcgcgcg atctgcgacc aggtgggcgc gactctcttc gccgacgtcg
    10741 cgcactacgc gggattgatc gtgaccgggc tttaccccag cccgatcggc atcgcggact
    10801 tcgtgaccag caccacgcac aagacgctgc gcggcccgcg cggcggcctc attctcgcgg
    10861 gcgccgagca cgagaaggcg ctcaattcct cgatcttccc caacctccag ggcggcccgc
    10921 tcatgcacgt gatcgccgcc aaggcggtcg cattcaagga ggcgctcgag cccgatttcc
    10981 gcgactacca ggtgcaggtc atgacgaatg cgaaggtgat ggcgaagacg ctgcagtcgc
    11041 gcggcgtgcg catcgtctcc gggcgcaccg agtcgcacct gttcctggtc gacctgcggg
    11101 cgaagaacgt gaccggcaag gacgccgagt ccgccctcgg gcgcgcgcac atcacggtga
    11161 acaagaacgc cattccaaac gatccgcaga agcccatggt gacgagcggt atccgcatcg
    11221 gctctccggc gatgaccacg cgcggcttcc gcgacgccga ggccgagcgc ctcgcgcacc
    11281 tgatcgccga cgtgctcgat gcgccgcagg acgaccgggt gctcaagcgc gtcgccggcg
    11341 aggtccagga gctgtgccgc aagttcccgg tctaccgtcc ggccgaggcg ggcgcgcagc
    11401 gcgtcgcggt cgcataagaa caaacggcag ggagcgacga tgaagtgccc gtactgcaag
    11461 tccgagaaca cgggggtgat cgactcgcgc ctctccgacg acggcgagag cgtgcgccgc
    11521 cgccggcaat gcgaggactg cgacaagcgc ttcaccacgt acgagcgcac cgagctctcc
    11581 atgccgatga tcgtcaagag caacgggaac cgcaccgcct acgaccgcga gaagatccgc
    11641 acgggcttca tgcgcgccct gcacaagcgc ccggttccca ccgagtacgt cgaccaggcg
    11701 atcaaccgcg tcgagcagcg tatcatggcg ctcggcgagc gcgaggtcgc gagccgcgtg
    11761 atcggcgaga tggtgatgcg cgagctgaaa aagatggacg acgtcgccta catccgcttc
    11821 gcctcggtat acgaggactt ccagcgcgtc gacgatttcc gcgacgcgat ccaggaagtg
    11881 aagcgcccgg gcaaggccaa ggcgcgccgc gcgccgaagt gatggccgac gccgccgacc
    11941 tcggcttcat gggtcgggcg ctcgagctgg cccgtctcgg gctgtatacg accacgccga
    12001 acccgcgcgt cggctgcgtc gccgtcaagg acggcgacgt ggtgggcgaa ggctggcacg
    12061 caaaggccgg cctcccgcac gccgaggtgc tcgcgctcgc gcaggcgggg gcgcgcggac
    12121 gcggcgcgac gctctacctc aacctcgagc cgtgcagcca ccacgggcgc acgccgccct
    12181 gcgccgacgc gctgatcgcg gcgggcgtgg cgcgcgtcgt ggccgcgatg caggatccga
    12241 atccccaggt cgcgggagcg ggcttcgcca ggctgcgcgc ggccgggatc cgcgtcgagc
    12301 acggcctgat ggaggaggag gcgcgagagc tcaatatcgg cttcgtgtcg cgcatgacgc
    12361 gcgggtgccc gtgggtacgg atgaaaatcg ccgcaagcct cgacggccgc accgctctcg
    12421 ccaacggcaa gagccagtgg atcaccggcg acgccgcgcg cctcgacgga cacgcctggc
    12481 gtgcgcgcgc ctgcgccatc ctcacgggca tcggcacggt caaggacgac gatccgcgcc
    12541 tcacggtgcg cggcgtcgac acgccgcgcc agccactgaa ggtgctcatc gactcgcggc
    12601 tcgaagtgga cccggcggcg cggctgttcg agcagggcgc agtcctcgtc gtcgcggcgc
    12661 gcgaggaccg tgcgaaggag gaggcgctgc gcgcgcgcgg cgccgaggtg atctgcatcc
    12721 cgaacgcgca gggcaagacc gagctggcgt cggtcatgcg cgagctcggc cggcgaggaa
    12781 tcaacgagct gcacgtcgag gcgggcaccc ggctgaacgg gtcgctgctg cgcgagggct
    12841 gcgtcgacga gttgctcgtg tacctcgcgc cgagcgtgat gggagacacg gggcgcggga
    12901 tgttcgcgct gcccgagccg gccgctctcg accggctcgt ccggctcgag atgcgcggcg
    12961 ccgagcgcat cggcgacgac ctccgcatcc tcgcccgcgt caggcgggac tgaacgacgc
    13021 ttctgccgcg gggcgcggcc cgagcaccgc ggcgatcacg tcctcgacca cctgcgcggt
    13081 gcgccggtcg acgtcgcgat gggggttgta ttccacaacc tcgagcgcga cgagcgcggc
    13141 gtccttcgcg cagcaggtcg cgagcgcctc gatcagcgcc ggcgcggcga tcccgccgcg
    13201 caccggcgtg ccgacaccgg gcacgtcgag cggatccatc gcgtcgagat cgagcgtgat
    13261 gccgtagccg gcggtgtcgg cgcgcgcgat gtcgatcgcc tcgcgcatca ccgcgtccag
    13321 cccgcgcgcg cgcagctcgt ccatgtgcac gatgcgcacg cccaggcgcg tgagcagcgc
    13381 ttcctcctcc ggctcgtggc tgcgcacgcc gaccaggcag acatggcgcg gcgcgatctg
    13441 tccttcgtgc aggatctcgg ccacctcgat cgcgagctcc cggctcgccc cgtggccgag
    13501 gagggccgcg agcggcatgc cgtgcggcat gccgctgtgg ctggtggccg gggtatggct
    13561 gtccatgtgc gcgtcgatcc acacgagccc gagcgggccc cgcggcgaga ccgcccgggc
    13621 cgcgccgctc catgttccga tcgcgcagct gtgatccccg cccacgacga ccggcaggcc
    13681 gcccgcgcgg atggtggcgc cgacttcgcg cgcgagccgg ttgcatatct ccgagatcgc
    13741 gcgcatgcgc gactgtcccg ggtggtgctc cggctccagg gtcgagcgcc aggacagctg
    13801 gacgccgcgg ccagcgaggc gcgcgagcag gccgtcgcgc tcgagcgcgg ccggtccgtc
    13861 gcgacagcgc gggtcgcgcg cgcccaggcc cgacgcgacg ccgatcaccg cggcgggaag
    13921 agggtgggtg ctgggatcgc tcatcgggtc ggatgcggca ccatcttgga ccgcaccgcg
    13981 gcggtggtgt tcgaacgccg gtagaatccg cgcgtgttta ccggcatcgt gacgacggtg
    14041 ggaaggatcg cgcgcctcga gcccgcggcg ggcggtgtgc gcgtcgcggt ggaagcgccg
    14101 cacctcggcc tgcaggacgt cgccatcggc gacagcatag ccgtgaacgg cgtgtgcctc
    14161 acggtggtat cgcgccaggc gtcgcgtttc gaggtcgagc tgtccgcgga aacgcttcga
    14221 tgcacggtcg gcttcgagct ctccggcgag gtgaatctcg agaaggcgct gaggctctcc
    14281 gaccgtctcg gcggtcatct ggtctcggga cacgtcgatg gcgtcggcga ggtgacgcgg
    14341 ctcgatgccg cgggcgataa ccgcgtgctc gcggtgcggg cgccggccgg cctcgagcgc
    14401 tacatcgcgc gcaagggctc gatcacgttg aacggcgtga gcctcacggt gaacgcggcg
    14461 aacggcgccg agttcgaggt caatctcatt ccgcacacgc tgcaggccac caacttgaag
    14521 cacttgcgca ccggcgcgcg cgtgaacctc gaggtcgacc tgctggcccg ctatctcgag
    14581 cgactggcgg ccgcgtccgg tcccgcgccc acaaagggcg cgcggggagc gctcccggcc
    14641 gaccacggcc ccgtgcccgc gccccgggcg aggggcgtaa c
//
LOCUS       DATDMW010000242         7168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_821627, whole genome shotgun sequence.
ACCESSION   DATDMW010000242 DATDMW010000000
VERSION     DATDMW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821627"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VI319_10360"
     CDS             <1..327
                     /locus_tag="VI319_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6864291.1"
                     /translation="IGEFQLMQGKLADMYATLSACRAYVYAVGQALDRGETTRKDAAG
                     AILYAAEKATWMAGEAIQCLGGMGYINETPTGRLWRDAKLYEIGAGTSEIRRWLIGRE
                     LFEETQ"
     gene            324..764
                     /locus_tag="VI319_10365"
     CDS             324..764
                     /locus_tag="VI319_10365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY6864292.1"
                     /translation="MTGLVETYRGTVYPWQCDHMGHMNVMWYVGKFDEATWNLFAALG
                     MTPAMLREQGRAMAAVQQNISYRRELLAGDTVAVRSGILEVRDKVVRFVHEMRNAASG
                     EIAAVCELTGVQIDAKSRKSSPFAADVLARARELATTYDFGRRA"
     gene            804..1991
                     /locus_tag="VI319_10370"
     CDS             804..1991
                     /locus_tag="VI319_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY6864293.1"
                     /translation="MSNDPIVIVSAARTPMGAFQGELKDFAAPQLGAAAIRAAVERAG
                     VKPESIDEVIMGCVLPAGQGQAPARQAALGAGLPLATGCTTINKMCGSGMKAAMLAHD
                     ILAAGSDGRVIVAGGMESMTNAPYLLPKARAGYRMGHQQVLDHMFFDGLEDAYDKGKL
                     MGVFAEDCAAKYAFSREAQDQFAVTSLERAQAANKNGSFAWEMTPMTVKAGKNERFLE
                     TDEQPFKANIEKIPTLKPAFRKDGSVTAANSSSISDGAAALVLMRRSTAEKLGAKPLA
                     TVVAHATHAQEPGWFTTAPVGAIRKLYERTGWKKDQVDLYEINEAFAVVTLAAMKEHG
                     LPHEKVNIHGGACALGHPIGASGARIVVTLIGALRKTGGRRGVAALCIGGGEATAMAI
                     ELA"
     gene            1999..3132
                     /locus_tag="VI319_10375"
     CDS             1999..3132
                     /locus_tag="VI319_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6864294.1"
                     /translation="MLLAAEHEQLRDAVRKFARERLAPGAARRDRERAFPREELAGLA
                     AMGLYGVAIPEPWGGAGMDATALALAIEEIAAGEGATATIVAVNNLVAGILNGYGSEE
                     QKRRFLVPLARGESLGAFCLTEPQAGSDASAIVTRADKHGGDYVLNGVKQFITSGKNA
                     DLAVVFAVTDKAAGKKGMSAFVVPTKTPGYKVARIEDKAGQHASDTAQIVLEDCRVPG
                     ANRLGEDGAGYRIALANLESGRINVAAQAVGMARAALEAAVAYAGERRSMGKTLAEHQ
                     AVAFRLADMATGVEAGRQLYLHAAALRDAGKPCLKEASMAKLFASETAERVCSDAIQI
                     HGGYGYVADFPVERIWRDVRVCRIYEGASDIQRMVIGRALTGE"
     gene            3134..3736
                     /locus_tag="VI319_10380"
     CDS             3134..3736
                     /locus_tag="VI319_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEY6864295.1"
                     /translation="MAKTIDFYFDFSSPYGYIASTKIDALAAKHGREATWRPFLLGAA
                     FKQTGGQPLTDIPIKGKYAARDMVRTAKFMGVDYRQPSTFPIGTVAAARAFYWADAKD
                     PKRARDVAKALYRAYFVDDVNIGDAEKTVEVCVRLGLDAAAVRAGLADPAVKDRLRAE
                     VDAAIALGAFGSPYIVVDGEPFWGSDRLEQVDRWLATGGW"
     gene            3747..5354
                     /locus_tag="VI319_10385"
     CDS             3747..5354
                     /locus_tag="VI319_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY6864296.1"
                     /translation="MPAIKTRLDVRSEEFQANAGAMSALVADLRARVERAAEGGDAQA
                     REKHVARGKLLPRERLRLLLDPGSPFLELSPLAALGLYGDEAPGAGLIAGIGRVSGQE
                     CVIVVNDATVKGGTYSPLTVKKHLRAQEIARDNGLPCIYLVDSGGAFLPEQDDVFPDR
                     EHFGRIFYNQANLSAAGIPQIAAVMGSCTAGGAYVPAMSDETVIVKGQGTIFLGGPPL
                     VKAATGEVVTPEELGGAEVHARQSGVADHYAQNDAHALAIVRSIVASLNRAKRVTLAL
                     REPAEPLYPASELGGVVPADVRKPYDVREVIARLVDASDFDEFKQSYGTTLVTGWARL
                     WGYPVGILASNGILFSESALKGAHFVELASQRGIPLLFLQNITGFMVGKKYEAGGIAK
                     DGAKLVTAVACTRVPKLTVIIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQA
                     AAVLATVRRDGIEAKGGSWSKAEEEAFRRPVLEQYERQGHPYYATARLWDDGIIAPED
                     TRRVLGLALSAALNAPIEPTRFGVFRM"
     gene            5369..6190
                     /locus_tag="VI319_10390"
     CDS             5369..6190
                     /locus_tag="VI319_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY6864297.1"
                     /translation="MGYQSIEIQMGDGAAVLWLNRPQLRNAFDENMIAEVTDAFDALE
                     RDPAVRAVVLAGKGKVFCAGADLDWMKRMKGMSREENREDALAFAKMLHRIYTLAKPT
                     LARVHGAAYAGGIGLLAACDIAVASNDTEFCLSEVKLGLAPATISPYVVAAMGERAAR
                     RYCVTAERFPAAEAYRIGLIQEITLPAELDATVNAILGELVQGAPGAQASAKEMLRQV
                     AGAPIDHRLIAQTAERIAALRVSDEGQEGMAAFFEKRNPAWVAPFAKSKAKRAKK"
     gene            6198..>7168
                     /locus_tag="VI319_10395"
     CDS             6198..>7168
                     /locus_tag="VI319_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY6864298.1"
                     /translation="MFEKLLIANRGEIACRVMRTAKRLGVRTVGVYSDADAGAMHVAL
                     ADEALRIGEAPARESYLRIDRIVAAALHAGADAVHPGYGFLSENAAFAEACERAGLVF
                     VGPPPGAIRAMGDKSAAKAIMAKAGVPLLPGYHGERQEPAFLLEQARAIGFPVMIKPC
                     AGGGGKGMRVVRSAGDFDATLAASRREAASAFGDPTVFCERYLENGHHVEVQILADAY
                     GMIWALGERECSVQRRHQKVLEEAPSPLVERLGGDLRERLLKAGRDAAAAVGYRGAGT
                     VEFLADERGGFWVLEMNTRLQVEHPVTECVTGVDLVGLQLEVAAGKPL"
BASE COUNT         1115 a         2490 c         2609 g          954 t
ORIGIN      
        1 atcggcgagt tccagctgat gcagggcaag ctcgccgaca tgtacgccac gctctcggcg
       61 tgccgcgcct atgtatatgc cgtgggccag gcgctcgacc gcggggaaac aacgcgcaag
      121 gacgccgccg gcgcgatcct ctacgcggcg gagaaggcga cctggatggc gggcgaggcg
      181 atccagtgcc tgggcggcat gggctacatc aacgagactc cgaccgggcg cctatggcgc
      241 gacgcgaagc tgtacgagat cggcgcgggc acgtccgaga tccggcgctg gctgatcggg
      301 cgcgagctct tcgaggagac gcaatgaccg gcctggtcga gacctatcgc ggcaccgtgt
      361 atccgtggca atgcgaccac atgggccaca tgaacgtcat gtggtacgtg ggcaagttcg
      421 acgaggcgac ctggaacctg ttcgcagcgc tcgggatgac gccggcgatg ctgcgcgagc
      481 agggccgcgc catggccgcc gtgcagcaga acatcagcta ccggcgcgag ctgctcgcgg
      541 gcgacacggt cgccgtgcgc tcgggcatcc tcgaggtgcg cgacaaggtc gtgcgcttcg
      601 tgcacgagat gcgcaacgcc gcgtcgggcg agatcgccgc ggtgtgcgag ctgaccggcg
      661 tgcagatcga cgcgaagagc cgaaagtcct cgcccttcgc ggcggacgtg ctcgcccgcg
      721 cccgcgagtt ggccaccacc tacgacttcg gccgccgcgc gtgagcccgg ctcgaaattc
      781 aacccatgca gcaaggaggc atcatgtcca atgacccgat cgtcatcgtt tcggccgcgc
      841 gcaccccgat gggcgctttc cagggcgagc tcaaggactt cgccgcgccg cagctcggcg
      901 ccgccgcgat ccgcgccgcg gtcgagcgcg ccggcgtcaa gcccgagagc atcgacgaag
      961 tcatcatggg ctgcgtcctt cccgcgggcc agggccaggc gcccgcgcgc caggccgcgc
     1021 tcggcgccgg tctgccgctc gcgaccggct gcacaacgat caacaagatg tgcggctcgg
     1081 gcatgaaggc ggcgatgctg gcgcacgata tcctcgccgc gggcagcgat gggcgcgtga
     1141 tcgtcgcggg tggcatggag tcgatgacca acgcgcccta cctcctgccg aaggcgcgtg
     1201 ccggctaccg catgggccac cagcaggtgc tcgaccacat gttcttcgac gggctcgaag
     1261 acgcgtacga caaaggcaaa ttgatgggcg tcttcgccga ggactgcgcc gcgaagtacg
     1321 cgttctcgcg cgaggcgcag gaccagttcg ccgtcacgtc gctcgagcgc gcgcaggccg
     1381 ccaacaagaa cggctcgttc gcctgggaga tgacgccgat gacggtcaag gccggcaaga
     1441 acgagcgctt cctcgagacc gacgagcagc cgttcaaggc gaacatcgag aagatcccga
     1501 cgctcaagcc tgcgttccgg aaggacggct cggtcaccgc cgccaactcg agctcgatct
     1561 ccgacggcgc cgcggcgctc gtcctgatgc gccgctcgac cgccgagaag ctcggcgcga
     1621 agccgctcgc gaccgtcgtc gcgcacgcga cgcacgcgca ggagcctggc tggttcacga
     1681 ccgcgcccgt cggggcgatc cgcaagctct acgagcgcac cggctggaag aaggaccagg
     1741 tcgacctcta cgagatcaac gaggcgttcg cggtcgtgac cctggcggcg atgaaggagc
     1801 acggcctgcc gcacgagaag gtgaacatcc acggcggcgc gtgcgcgctc gggcacccga
     1861 tcggggcgtc gggggcgcgc atcgtcgtca cgctgatcgg cgcgctccgg aagaccggag
     1921 gccggcgcgg cgtcgcggcg ctctgcatcg gcggcggcga ggcgaccgcg atggccatcg
     1981 agctggcgta aaagcgacgt gctcctcgcg gccgagcacg agcagctgcg cgacgcggtg
     2041 cgcaagttcg cgcgcgagcg gcttgcgccg ggcgcggcgc gccgcgaccg cgagcgcgcg
     2101 ttcccgcgcg aggagctcgc cgggctcgcc gcgatgggcc tctacggcgt cgcgatcccc
     2161 gagccctggg gcggcgcggg aatggacgcg accgcgctcg cgctggcgat cgaggagatc
     2221 gccgccggcg agggcgcgac ggcgaccatc gtcgcggtca acaacctcgt cgccgggatc
     2281 ctgaacggct acggcagcga ggagcagaag cggcgctttc tggtcccgct cgcgcgcggc
     2341 gagagcctcg gcgcgttctg cctcaccgag ccgcaggccg gctccgacgc ctcggcgatc
     2401 gtcacccgcg ccgacaagca cggcggcgac tacgtgctga acggcgtcaa gcagttcatc
     2461 acctcgggca agaacgccga cctcgccgtt gtcttcgccg tcaccgacaa ggcggccgga
     2521 aagaaaggaa tgagcgcttt cgtcgtgccg acgaagacgc cgggctacaa agtcgcgcgc
     2581 atcgaggaca aggccggcca gcacgcgtcc gacaccgcgc agatcgtcct cgaggactgc
     2641 cgcgttccgg gggcgaaccg cctgggggag gacggcgcgg gctaccgcat cgcgctcgcg
     2701 aacctcgagt cggggcggat caacgtcgcg gcgcaggcgg tgggcatggc gcgcgccgcg
     2761 ctcgaggccg cggtcgcgta cgcgggcgag cgcaggagca tggggaagac gctcgccgag
     2821 caccaggccg tggcctttcg cctggccgac atggcgacgg gcgtcgaggc cgggcggcag
     2881 ctctatctcc acgccgcggc gctgcgcgac gcgggcaagc cgtgcctcaa ggaggcgtcg
     2941 atggccaagc tcttcgcctc cgagaccgcc gagcgggtct gctcggacgc gatccagatc
     3001 cacggcggct acggctacgt cgccgacttt cccgtggaga ggatctggcg cgacgtgcgc
     3061 gtgtgcagga tctacgaggg cgcgagcgac atccagcgga tggtgatcgg ccgcgcgctc
     3121 accggagaat gacatggcga aaacgatcga cttctacttc gacttctcct cgccctacgg
     3181 ctacatcgcg agcacgaaga tcgacgcgct cgcggcgaag cacgggcgcg aggcgacctg
     3241 gcggccgttc ctgctcggcg ccgcgttcaa gcagacgggc ggccagccgc tcaccgacat
     3301 cccgatcaag ggcaagtacg cggcgcgcga catggttcgc accgcgaaat tcatgggtgt
     3361 cgactaccgc cagccgtcga cgttcccgat cggcaccgtc gcggcggcgc gcgcctttta
     3421 ctgggccgac gcgaaggacc cgaagcgcgc ccgggacgtg gcgaaagcgc tgtaccgcgc
     3481 ctacttcgtc gacgacgtga acatcggcga cgccgagaag acggtcgagg tctgcgtcag
     3541 gctgggcctc gacgccgcgg cggtgcgcgc cgggctcgcc gacccggccg tcaaggaccg
     3601 gctgcgcgcc gaggtcgacg ccgcgatcgc gctcggcgcc ttcggctcgc cctacatcgt
     3661 ggtcgacggc gagccgttct ggggcagcga ccgcctcgag caggtcgacc gctggctcgc
     3721 gaccggcggc tggtaatctg cgcgcgatgc ccgcgatcaa gacaaggctc gacgtccgct
     3781 cggaggagtt ccaggccaac gccggcgcga tgtccgcgct cgtcgccgac ctgcgcgccc
     3841 gcgtcgagcg cgcggccgaa ggcggcgatg cccaggcacg cgagaagcac gtcgcgcgcg
     3901 ggaagctgct gccgcgcgag cggctgcggc tgctgctcga tcccggctca ccgttcctcg
     3961 agctctcgcc gctcgccgcc ctcggcctct acggggacga ggcgccgggc gccggcctca
     4021 tcgccggcat cggccgcgtc tcgggccagg agtgcgtgat cgtcgtcaac gacgcgacgg
     4081 tcaagggcgg cacctactcc cccctgacgg tgaagaagca cctgcgcgcg caggagatcg
     4141 cccgcgacaa cggccttccc tgcatctacc tcgtggactc gggcggcgcg ttcctgcccg
     4201 agcaggacga cgtcttcccg gaccgcgagc acttcgggcg catcttctac aaccaggcga
     4261 acctgtcggc ggcggggatt ccgcagatcg cggcggtgat gggctcgtgc accgcgggcg
     4321 gcgcctacgt gccggcgatg tcggacgaga cggtgatcgt gaagggacag ggcacgatct
     4381 tcctcggcgg gccgccgctc gtgaaggccg cgaccggcga ggtcgtcacg cccgaggagc
     4441 tgggcggcgc cgaggtgcac gcgcggcagt ccggcgtcgc cgatcattac gcccagaacg
     4501 acgcgcacgc gctcgcgatc gtgcgctcga tcgtcgccag cctgaaccgc gcgaagcgcg
     4561 tgacgctcgc gctgcgcgag cccgcggagc cgctctaccc cgcgtccgag ctcggcggcg
     4621 tcgttcccgc cgacgtgcgc aagccctacg acgtgcgcga ggtgatcgcg cgcctcgtgg
     4681 acgcgagcga cttcgacgag ttcaagcaga gctacggcac gacgctcgtg accgggtggg
     4741 cgcgcctctg gggctacccg gtcggcatcc tcgcgagcaa cggcatcctc ttttccgagt
     4801 ccgcgctcaa gggcgcgcat ttcgtggagc tcgccagcca gcgcggcatc cccttgctct
     4861 tcctgcagaa catcacgggc ttcatggtcg ggaagaaata cgaggcgggc ggcatcgcca
     4921 aggacggcgc gaagctcgtg accgcggtcg cgtgcacgcg cgtccccaag ctcaccgtga
     4981 tcatcggcgg ctcgttcggc gccggcaact acggcatgtg cgggcgcgcc tactcgccgc
     5041 gcttcctgtg gatgtggccg aacgcgcgca tctcggtgat gggcggcgag caggcggccg
     5101 cggtgctcgc gaccgtgcgc cgcgacggaa tcgaggcgaa gggcggcagc tggagcaagg
     5161 ccgaggagga ggcgttccgg cgtccggtcc tcgagcagta cgagcgccag gggcatcctt
     5221 actatgcgac cgcgcgcctg tgggacgacg gtatcatcgc gcccgaggac acgcgccgcg
     5281 tgctcgggct cgcgctctcg gcggcgctca acgcgccgat cgagccgacc cggttcggcg
     5341 tcttccggat gtaggaacaa gggaaaacat gggctaccaa tcgatcgaga tccagatggg
     5401 cgacggcgcc gccgtgctgt ggctgaaccg cccgcagctg cgcaacgctt tcgacgagaa
     5461 catgatcgcc gaggtgacgg acgcgttcga tgcgctcgag cgcgatccgg cggtgcgcgc
     5521 ggtcgtgctc gccggaaagg gcaaggtctt ctgcgcggga gccgacctcg actggatgaa
     5581 gcgcatgaaa ggcatgagcc gcgaggagaa ccgcgaggac gcgctcgcct tcgcgaagat
     5641 gctccaccgc atctacacgc tcgcgaagcc gacgctggcg cgcgtgcacg gcgcggcgta
     5701 cgcgggcggc atcggcctgc tcgccgcgtg cgatatcgcg gtcgcctcga acgacaccga
     5761 gttctgtctc tcggaagtca agctcggcct cgcgcccgcc accatcagcc cttacgtggt
     5821 cgccgcgatg ggcgagcgcg ccgcgcgccg ctactgcgtc accgcggagc gcttccccgc
     5881 ggcggaggcg taccgcatcg gcctgatcca ggagatcacg ctgcccgcgg agctcgacgc
     5941 cacggtgaac gcgatcctcg gcgagctggt gcagggcgcg ccgggcgcgc aggcatcggc
     6001 caaagagatg ctgcgccagg tggcgggcgc gccgatcgac caccggctga tcgcgcagac
     6061 cgccgagcgc atcgccgcgc tgcgcgtctc cgacgagggc caggagggca tggccgcctt
     6121 cttcgagaag cggaatccgg cgtgggtggc tccgttcgcc aagagcaagg cgaagcgcgc
     6181 gaagaagtga cgcactcatg ttcgagaagc tgctgatcgc gaaccgcggc gagatcgcgt
     6241 gccgcgtgat gcgcacggcg aagcgcctcg gcgtgcgcac cgtcggggtc tactccgacg
     6301 ccgacgcggg cgcgatgcac gtcgcgctcg ccgacgaggc gctccgcatc ggcgaggccc
     6361 cggcgcgcga gagctacctg cgcatcgacc gcatcgtcgc ggccgcgctg cacgccggcg
     6421 cggacgcggt gcacccgggc tacggcttcc tctccgagaa cgcggcgttc gccgaggcct
     6481 gcgagcgcgc ggggctcgtc ttcgtcggcc cgccgcccgg cgcgatccgc gccatgggcg
     6541 acaagtcggc ggcgaaggcg atcatggcga aggcgggcgt cccgctcctg cccggctacc
     6601 acggcgagcg gcaggagccc gccttcctgc tcgagcaggc gcgcgcgatc ggcttcccgg
     6661 tgatgatcaa gccctgcgcg ggcggcggcg gcaagggcat gcgcgtcgtg cggagcgcgg
     6721 gcgatttcga cgcgacgctc gcggcgagcc ggcgcgaggc cgcgtccgcg ttcggcgacc
     6781 cgaccgtgtt ctgcgagcgc tacctcgaga acggtcacca cgtcgaagtt caaattcttg
     6841 ccgacgcgta cgggatgatc tgggccctcg gcgagcggga gtgctcggtg cagcggcgcc
     6901 accagaaggt cctcgaggag gcgccgtcgc ccctggtcga gcggctcggc ggggacctgc
     6961 gggagcggct gctcaaggcg ggccgggacg cggcggccgc ggtcggctac cgcggggcgg
     7021 ggacggtcga gttcctggcc gacgagcggg gcgggttctg ggtcctggag atgaacacgc
     7081 gcctgcaggt cgagcacccg gtgaccgagt gcgtcaccgg cgtggacctg gtcggcctcc
     7141 agctcgaggt ggccgccggc aagccgct
//
LOCUS       DATDMW010000243        14149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2441979, whole genome shotgun sequence.
ACCESSION   DATDMW010000243 DATDMW010000000
VERSION     DATDMW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14149
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2441979"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="VI319_10400"
     CDS             complement(<1..85)
                     /locus_tag="VI319_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY6864299.1"
                     /translation="MAKAEAEIVNKLGLHARASAKLTQVASR"
     gene            complement(66..470)
                     /locus_tag="VI319_10405"
     CDS             complement(66..470)
                     /locus_tag="VI319_10405"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEY6864300.1"
                     /translation="MVGILIVGHGAFGEALLHCCTHVLGQRPSQIAQLAVAVNDDPEK
                     VLPRARALLAQIDGGDGVLVLTDVLGATPSNIAARLLAPGRIEGVAGVSLPMLIRALT
                     YRREPLATVIDKAVSGGCEGVVRMNGNGESGS"
     gene            complement(484..1428)
                     /gene="gshB"
                     /locus_tag="VI319_10410"
     CDS             complement(484..1428)
                     /gene="gshB"
                     /locus_tag="VI319_10410"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY6864301.1"
                     /translation="MKLLFVVDPPSSFKIYKDSTYAMMVEADKRGHELHFATLDGISW
                     KGGRVIAQSRPMELLAGKDPWYRAGAFAKAPLAEFDAVLMRKDPPFDMEYVVATWLLE
                     LAQREGAKVFNDPGAIRDHSEKLAIAQFPGLAPPTLVTRLAGEIHEFIAAHGDVILKP
                     LGGMGGDSVFRVTDADFNRNVLIETLTHHGARAIMIQRYVPEIRDGDKRVLLIAGKPV
                     PHCLARMPRPGESRANLAAGGTGVARPLSPRDREIAESLGPELARRGLLLVGLDVIGD
                     FLTEINVTSPTCFQEIAQQTGFNVAGMFMDAVEAAVSK"
     gene            complement(1472..2761)
                     /locus_tag="VI319_10415"
     CDS             complement(1472..2761)
                     /locus_tag="VI319_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY6864302.1"
                     /translation="MSGWIIFIGLILLMLTGMPISIALGLTVLAFLFFMTDMPVEIVS
                     QRLFTGLDQFAIMAIPFFILAGSFLTHGGVARRMIRFASALVGHLYGGLAMAGVFACA
                     LFAAVSGSSPATVVAIGTILIPAMEKQGYPRRFGVGAIGTAGVLGILIPPSIVMVIYS
                     VSTNTSVGRLFIAGIVPGLLLAAILMGIVWVVAKRRNYPRMPRADLKEIFASFWDAIW
                     GLLLIVVVIGGIYGGVFTPTEAAAMSAVYAFIIAVFVYRDLKLADVPRVLLDAANMSA
                     MLLYIITNAILFSFLLTSENIPQAMAQWIIDKHLSAWVFLMGCNVLLLIAGNFMEPSS
                     IVLLLAPILFPVAVKLGIDPIHFGIIMTINMEVGLIHPPVGLNLYVASGIARMGLTET
                     TVAFAPWIVIMVLYLLLVTYVPDIAMWLPNAIYHRPG"
     gene            complement(2758..3258)
                     /locus_tag="VI319_10420"
     CDS             complement(2758..3258)
                     /locus_tag="VI319_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY6864303.1"
                     /translation="MGLATLIIFASVLYRYTGDIPWIWQYTQVLNLTWAQELCIYLFI
                     WMAKFGAAYGVRTGIHVGVDVLINRLPEKQRRVGVLLGLGAGAFFTAVVAALGAKFVW
                     LMYGTGQVSPDLELPMWIVYLAVPLGSALMCYRFLQVAWRFRRGEPLPSHNHARVEGL
                     QAEGRG"
     gene            complement(3361..4380)
                     /locus_tag="VI319_10425"
     CDS             complement(3361..4380)
                     /locus_tag="VI319_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY6864304.1"
                     /translation="MKVRHVSLAVVAAFAAGLAATAAHAQAPIVLKFSHVVAEDTPKG
                     KGALKFKELAEAKTRGRVKVEVYPNSQLYKDREELEALQLGAVQMLAPSVSKFGPLGV
                     RQFEVYDLPYMFPTQDVLDKVIDGPVGKRLFKLLEPKGIVGLGYWSNGFKEFSSNRPM
                     KKLADFKGMKIRIQSSKVLEGEIRALGAIPQVMAFSEVYTALQQGVVDGEENSISNKY
                     TQKMHEVQKHITMSDHGVIMYAVIVNKKFWEGLPSDVHKELQAAMDEASKYERSIAQK
                     ENDDALEKIREAKTTEIYVLPASEKAVWRKAMLPLYKQYEGSVGKDNLDAIEKIVAES
                     SKKKM"
     gene            complement(4418..4798)
                     /locus_tag="VI319_10430"
     CDS             complement(4418..4798)
                     /locus_tag="VI319_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6864305.1"
                     /translation="MIDHLDHIVLTTARTRECIDFYTRTLGMTLERFGEGRMALKFGA
                     QKINLHEKGREFEPKATTPVPGALDFCFIASVPLDEVIARLSAAGIPLVEGPVMKTGA
                     CYPIRSVYVRDPDGNLVEISERAG"
     gene            complement(4815..5705)
                     /locus_tag="VI319_10435"
     CDS             complement(4815..5705)
                     /locus_tag="VI319_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6864306.1"
                     /translation="MAAPDIPPHTSPIRTPKQLVIVVLLAFIVPITIILALAYLVTSG
                     PRPDPDSVALSPEKVAERIRPVAEVTIGSARGSQAAKSGDEVYKTVCQACHAAGIQGA
                     PKFGNRGDWGPRIKRGEKALLQSALKGKGAMPPKGGAADLSDVEIERALVYMANAAGA
                     GFKEPAAPAQNAAPAPQTAQAQPAPAADAAGHDHDHGAPTAPAPAAPAAKATPASAKA
                     DGKKVFQGTCVVCHGTGVAGAPKFGDKAAWAPHLAHGKDELYHSALTGKNAMPPKGGN
                     MTLSDDEVKAAVDYMVGAVK"
     gene            5767..5842
                     /locus_tag="VI319_10440"
     tRNA            5767..5842
                     /locus_tag="VI319_10440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5800..5802,aa:Arg,seq:ccg)
     gene            5913..6872
                     /locus_tag="VI319_10445"
     CDS             5913..6872
                     /locus_tag="VI319_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864307.1"
                     /translation="MALLLVTTAALAGGSSYGIAPGSRQISGKVSEWPVPTPKFARDP
                     APGPDGNIYITVMQGDRIARFDPRRQVFNEWDVPAGAHPHGLVVDRDGKVWYTGNGNG
                     TIGELDPASGKVTQHKVPSGGSPHTIVIADDGALWFTNQSGSVGRLDRRSGRITEVRM
                     PGGPYGIALDRQGNVWVCRMNADRLGRINPATGQVSELFMGSGSRPRRMAAAPDGTLW
                     VTLYGTGHLAHVDPATSKVIREYRMPAGDDGGPYAVTVDGAGRVFASEIQSDTVAMLD
                     PKTETFQVYRLPSKNVGIRKAIVDAQGRFWYMGSHNGRLGVIE"
     gene            6917..7897
                     /locus_tag="VI319_10450"
     CDS             6917..7897
                     /locus_tag="VI319_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HEY6864308.1"
                     /translation="MHGFRATKFLIAAAAALWAGGALAQNYPTRAVTMYVPYAAGGPT
                     DIVARVVAQAMTKPLGQTVLVENRPSSGGFLAPQQVKDAKPDGYSILIHHIGMATIPI
                     LYRQPKFDPLKDFDYIGLINEVPMTIIARQNFPAKNFREFLSYIKANKSKVTLANAGI
                     GAASHLCGLLFMSAIQTDFLTVPYKGTGPAMNDLLGGQVDFMCDQTTNTTSPIKSGKV
                     KAYAVTTPKRLASLPDIPTLDESGVKGADVTVWHALYAPKGTPKPVIDKLVSALQAAL
                     KDKTVNERFAELGAVTVSVDRQNPQALEKFLKSEIDKWGPIIRKAGQYAD"
     gene            7924..8916
                     /locus_tag="VI319_10455"
     CDS             7924..8916
                     /locus_tag="VI319_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6864309.1"
                     /translation="MRRVNIALTAALAAAALAGCAGMTPSGGSAGPGVVKVTALGSHD
                     GEFCSRDRALVFEDPDGTRILYDAGFTVRGADDPRLGRIDAVLISHLHGDHLGPAHQP
                     SANAGACGNPQFSVSDVPNSNTVNIVVAKRAKFLVGGDMNTWFAHKVKAAGGDPAQVQ
                     LVRFGASAKVGGVKVTTVPAAHTNAVDPAFIDKPLADLLRANGLLAEVGPPVGYVVSF
                     SNGLVVYLSGDTGVIAEQDVVVRRYYKANLAVINIGDVFTTGPSEAAYVIDDLVKPSS
                     VIASHANEVATKGGKVIPGTRTATFMKLVHVPAYVPLSGHTMSFDGGGKCVAGC"
     gene            8981..9358
                     /locus_tag="VI319_10460"
     CDS             8981..9358
                     /locus_tag="VI319_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY6864310.1"
                     /translation="MRLLLLWLINAVTLLLIPYLMPSVSVSSFYTALITALILGLVNS
                     VIRPVLVLLTLPITIVTLGLFILVINGLMFWFVASFIEGFHVEGFWSAFWGAIVYGIL
                     STVFSAILLPRHHPPPPPVRRDV"
     gene            complement(9355..10821)
                     /gene="rng"
                     /locus_tag="VI319_10465"
     CDS             complement(9355..10821)
                     /gene="rng"
                     /locus_tag="VI319_10465"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEY6864311.1"
                     /translation="MIEDILINCTPQETRVAVMQSGVVQELHVERALARGIVGNIYAG
                     KIARVLPGMQSAFVEIGLERAAFLHVADIWEHRRAAQQSPAAAKPIEKILAEGQSILV
                     QVIKDPIGTKGARLSTQISIAGRLLVYLPQDPHIGISQRIEDEAERERLRGRLQHLLP
                     EDEAGGFIVRTVAETATEAELAADVSYLRRLWHDIRGRALTSSAPALLYQDLSLAQRV
                     LRDFVSERTGRIVIDSRENFQKLQGFAGEYMPNVLPRVEHYSGERPLFDLHNVEDEIE
                     HALARRVELKSGGYLVIDQTEATTAIDVNTGGYVGVRNFDDTIFKTNLEAAQVIARQL
                     RLRNLGGIIIIDFIDMENPEHRAAVLEEFNKTLARDRTKMTVNGFTALGLVEMTRKRT
                     RESLEHVLCEPCPTCSGRGVVKTAQTVCYEILREILREARQFSAREYRILASQSVIDL
                     FLEEESQALAMLGDFIGKPVSMQVETSYTPEQFDIILI"
     gene            complement(10835..11473)
                     /locus_tag="VI319_10470"
     CDS             complement(10835..11473)
                     /locus_tag="VI319_10470"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY6864312.1"
                     /translation="MATPAKPPAIAPKFYLASRSPRRRELLKQIGIAFEVILLREQPA
                     RGPDVNEAALPSEKPDDYVRRIGKLKADVGWDRVVQRQLPRQPVLAADTTVCVDEQIL
                     GKPVDRADAARILALLSGREHRVLTAVAIRYESRSKMTVNETVVRFRKISDADIQSYI
                     DTGEPMDKAGAYAIQGRAAAFVPELRGSYSGVMGLPLYETAQLIETFRAEGG"
     gene            complement(11550..12017)
                     /gene="rlmH"
                     /locus_tag="VI319_10475"
     CDS             complement(11550..12017)
                     /gene="rlmH"
                     /locus_tag="VI319_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY6864313.1"
                     /translation="MKLLAVAVGARMPRWVDEAFAEYARRMPRTLALELVEVRPEPRS
                     AGRTPAQILAAEAARIERALPARARRVVLDERGRDYTTAEFARWLEERRGEGDDLAFL
                     IGGPDGLAAEIKRGAASMRLSSLTLPHGLARVVLAEQLYRAASLLAGHPYHRE"
     gene            complement(12014..12415)
                     /gene="rsfS"
                     /locus_tag="VI319_10480"
     CDS             complement(12014..12415)
                     /gene="rsfS"
                     /locus_tag="VI319_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY6864314.1"
                     /translation="MTPIRKLEAAVVDALEDVKAKDIRVYDVAHLSSLFDRVVIASAE
                     SNRQLRALANSVREKTKAAGGRIVGVEGEESGEWVLVDLGDIVVHIMQPAVRVHYNLE
                     ELWDQPKPRPARGTAARAKRPAGTRKARKPA"
     gene            complement(12412..13047)
                     /gene="nadD"
                     /locus_tag="VI319_10485"
     CDS             complement(12412..13047)
                     /gene="nadD"
                     /locus_tag="VI319_10485"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY6864315.1"
                     /translation="MGGTFDPVHFGHLRLADEAVEILRLECVRWIPAGQPSHRNPPMA
                     AAADRLEMVKLAIARHPSFELDDAEIRQTTPSYTVETLERLRRELGEKRSIVLLLGTD
                     SFRNLTTWERWRDLFELAHIFVGLRPGNSLTPGGMPAELAAQWRERSGTPEEIVARPA
                     GRVVIYATTALDISATAIRRHFAQGLLPRYLLPAAVLEYIESHGIYTGNRA"
     gene            complement(13097..>14149)
                     /locus_tag="VI319_10490"
     CDS             complement(13097..>14149)
                     /locus_tag="VI319_10490"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY6864316.1"
                     /translation="DRLTLTPKSVEAMAAGLEEVAKLADPVGEVSELRERPSGIKVGR
                     MRVPLGVIGIIYESRPNVTADAAALCLKSGNACILRGGSESVRSNLAIAACMHEGLRA
                     GGLPENAVQVVDTPDRAAVGILLSMNEHVDIIVPRGGKSLIERVMRESRIPTIKHLDG
                     VCHVYIDDRADIDQAIRIADNAKTQRYGVCNAMETLLVAEGVADKVLPKLAKIYFDKG
                     VQLRGDERARALVPLMKAATEEDWYAEYLAPILAVRVVRGLDEAIAHIARYGSQHTDA
                     IVTEDRARAERFLREVDSSSVLVNASTRFADGFEYGLGAEIGISTDKLHARGPVGLEG
                     LTSQKFVVYGSGQVRG"
BASE COUNT         2196 a         4784 c         4915 g         2254 t
ORIGIN      
        1 agcggctcgc gacctgggtg agcttcgccg aggcgcgcgc gtgcagcccg agcttgttca
       61 cgatctcagc ttccgctttc gccattcccg ttcatccgca ccacgccctc gcagccgccg
      121 ctcacggcct tgtcgatcac ggtcgcgagc ggctcgcgcc ggtacgtgag cgcgcggatc
      181 agcatcggca gcgacacgcc ggcgacgccc tcgatccgcc ccggggccag cagccgcgcc
      241 gcgatgttcg acggcgtggc gccgagcacg tcggtcagca ccagcacgcc gtcgccgccg
      301 tcgatctgcg cgagcagcgc gcgcgcgcgg gggagcacct tctcgggatc gtcgttgacc
      361 gcgaccgcga gctgcgcgat ctgcgaaggc cgctggccga ggacgtgcgt gcagcagtgc
      421 agcagcgcct cgccgaaggc gccgtggccg acgatcagga tgccgaccat ggcttcgctt
      481 cgctcatttc gaaacggccg cttccaccgc gtccatgaac atcccggcga cgttgaaccc
      541 ggtctgctgc gcgatctcct ggaagcacgt ggggctggtg acgttgatct cggtgaggaa
      601 gtcgccgatc acgtcgaggc cgacgagcag cagcccgcgg cgcgcgagct cgggaccgag
      661 gctctcggcg atctcgcggt cgcgcgggga gagcggccgc gcgacgccgg tgccgcccgc
      721 cgcgaggttc gcgcgcgatt cgcccgggcg cggcatgcgc gccaggcagt gcgggacggg
      781 cttgcccgcg atcagcagca cgcgcttgtc gccgtcgcgg atctcgggga cgtagcgctg
      841 aatcatgatg gcgcgcgcgc cgtgatgcgt gagcgtctcg atgaggacgt tgcggttgaa
      901 atccgcgtcg gtgacgcgga agaccgagtc gccacccatg ccgccgagcg gcttcaggat
      961 gacgtcgccg tgcgccgcga tgaactcgtg gatctccccc gcgagccgcg tcacgagcgt
     1021 cggcggcgcg aggccgggga actgcgcgat cgcgagcttc tcgctgtggt cgcggatcgc
     1081 gcccgggtcg ttgaacacct tcgcgccttc gcgctgcgcc agctcgagca gccaggtggc
     1141 gacgacgtat tccatgtcga agggcgggtc cttgcgcatg aggaccgcgt cgaactcggc
     1201 gagcggcgcc ttggcgaagg ccccggcgcg gtaccagggg tccttcccgg cgagcagctc
     1261 catcggccgc gactgagcga tgacccggcc gcccttccag gagatgccgt cgagcgtcgc
     1321 gaaatgcagc tcgtggccgc gcttgtcggc ctcgaccatc atcgcgtagg tcgagtcctt
     1381 gtagatcttg aacgaggacg gcggatcgac gacgaagagc agcttcatgg cgacgaaatt
     1441 ctagccccaa acgcgcgggc cggtcgccgg atcagcccgg gcggtgatag atcgcgttcg
     1501 gcagccacat cgcgatgtcc gggacgtaag tcacgagcag caggtacagg accatgatga
     1561 cgatccacgg cgcgaacgcg acggtcgtct cggtcagccc catgcgcgcg atgccgctcg
     1621 cgacgtagag gttcaggccg accggcgggt ggatcaggcc gacctccatg ttgatggtca
     1681 tgatgatgcc gaaatggatc gggtcgatgc cgagcttcac cgcgaccggg aacaggatcg
     1741 gcgcgagcag cagcacgatc gacgagggct ccatgaagtt gccggcgatc agcagcagca
     1801 cgttgcagcc catcaggaaa acccaggccg acaggtgctt gtcgatgatc cactgcgcca
     1861 tcgcctgcgg gatgttctcg ctcgtcagca ggaacgagaa caggatcgcg ttggtgatga
     1921 tgtagagcag catggcgctc atgttggccg cgtcgagcag cacgcgcggc acgtcggcga
     1981 gcttcaggtc gcggtagacg aacacggcga tgatgaaggc gtacacggcg ctcatcgcgg
     2041 cggcctcggt gggcgtgaac acgccgccgt agatgccgcc gatcacgacg acgatcagca
     2101 gcagccccca gatcgcgtcc cagaacgagg cgaagatctc cttcagatcc gcgcgcggca
     2161 tgcgcgggta gttgcgccgc ttcgccacca cccagacgat gcccatcagg atcgccgcga
     2221 ggagcaagcc cggcacgatc ccggcgatga acagccggcc gacgctcgtg ttggtcgaca
     2281 ccgagtagat caccatcacg atcgaaggcg ggatcaggat gccgaggacg cccgcggtgc
     2341 cgatcgcgcc cacgccgaag cggcgcgggt agccctgctt ctccatggcc gggatcagga
     2401 tggtgccgat ggcgaccacc gtcgccgggc tcgagcccga gacggcggcg aagagcgcgc
     2461 acgcgaacac gcccgccatg gcgagcccgc cgtagaggtg gccgacgagc gcgctcgcga
     2521 agcggatcat gcggcgcgcg acgccgccgt gcgtgaggaa cgatccggcg agaatgaaga
     2581 acgggatcgc catgatcgcg aactggtcga gccccgtgaa cagccgctgc gacacgatct
     2641 cgaccggcat gtcggtcatg aagaacagga acgcgagcac cgtcagtccg agcgcgatcg
     2701 agatcggcat cccggtcagc atcagcagga tcagcccgat gaagatgatc cagccgctca
     2761 tccgcggccc tccgcctgca ggccctcgac gcgcgcgtgg ttgtgcgagg ggagcggctc
     2821 gccgcgccgg aagcgccagg ccacctgcag gaagcggtag cacatcagcg ccgagccgag
     2881 cgggaccgcg aggtagacga tccacatcgg cagctcgagg tcgggcgaca cctgcccggt
     2941 gccgtacatg agccagacga acttggcgcc gagcgccgcc accaccgccg tgaagaaggc
     3001 gcccgcgccg agcccgagga gcacgcccac gcggcgctgt ttttccggca gccggttgat
     3061 cagcacgtcg acgccgacgt ggatcccggt gcgcaccccg taggccgcgc cgaacttcgc
     3121 catccagatg aagaggtaga tgcacagctc ctgagcccac gtgaggttca ggacctgcgt
     3181 gtactgccag atccacggga tgtcgccggt gtagcgatac agcaccgacg cgaaaatgat
     3241 cagggtcgcg agccccatga aggtcgcgat cagccactcc tcgaggtggt cgaggatgcg
     3301 gttcagcaag gcggcgtcct cccggcggct ctacgcggcc gcccgcgcgt caatccgtgc
     3361 ctacatcttc ttcttcgaag actcggcgac gatcttctcg atggcgtcga ggttgtcctt
     3421 gccgaccgag ccctcgtact gcttgtagag cggaagcatg gccttgcgcc agacggcctt
     3481 ctccgaggcg ggcaggacgt agatctcggt ggtcttcgct tcccggatct tctcgagcgc
     3541 gtcgtcgttc tccttctgcg cgatcgagcg ctcgtacttg ctcgcctcgt ccatcgcggc
     3601 ctggagttcc ttgtgcacgt ccgagggcag tccttcccag aacttcttgt tgacgatgac
     3661 cgcgtacatg atcacgccgt ggtccgacat ggtgatgtgc ttctgcacct cgtgcatctt
     3721 ctgcgtgtac ttgttcgaga tgctgttctc ctcgccgtcg acgacgccct gctgcagcgc
     3781 cgtgtacacc tcggaaaacg ccatcacctg cgggatcgca ccgagcgcgc ggatctcgcc
     3841 ctcgagcacc ttcgaggact ggatgcggat cttcatgccc ttgaagtcgg cgagcttctt
     3901 catcggacgg ttcgaggaga actccttgaa gccgttgctc cagtagccga ggccgacgat
     3961 gcccttgggc tcgagcagct tgaacaggcg cttgccgacc gggccgtcga tcaccttgtc
     4021 gagcacgtcc tgcgtcggaa acatgtacgg caggtcgtac acctcgaact ggcgcacgcc
     4081 gagcggcccg aacttggaga ccgagggcgc cagcatctgc accgcgccga gctgcagggc
     4141 ctcgagctcc tcgcggtcct tgtacagctg gctgttggga tagacctcga ccttgacgcg
     4201 gccccgggtc ttcgcctcgg cgagctcctt gaacttgagc gcgcccttgc ccttgggcgt
     4261 gtcctcggcc acgacgtggc tgaacttgag aacgatcggc gcttgcgcgt gcgccgcggt
     4321 tgcggccagg ccggcggcga aggcggcgac gaccgccagc gagacgtgac gaaccttcat
     4381 gttcctcctc ctttttctcc ctgcgggtgg cggcggctca accggcccgc tctgagatct
     4441 cgaccaggtt cccgtccggg tcgcggacgt agaccgagcg gatagggtag cacgctcccg
     4501 ttttcatgac cggaccctcg accaagggta tacccgcggc cgagagccgc gcgatcacct
     4561 cgtcgagcgg caccgaggcg atgaagcaga agtccagggc gcccgggacc ggggtcgtcg
     4621 ccttgggctc gaactcccgg cccttctcgt gcaggttgat cttctgcgcg ccgaacttga
     4681 gcgccatgcg gccttcgccg aagcgctcga gcgtcatgcc gagcgtgcgc gtgtagaaat
     4741 cgatgcactc gcgcgtgcgc gcggtggtga ggacgatgtg gtcgaggtga tcgatcatgc
     4801 cgcgtgcgcc gcggctactt caccgcgccg accatgtagt ccacggcggc cttcacctcg
     4861 tcgtccgaga gcgtcatgtt gccgcccttc ggcggcatcg cgttcttccc ggtgagcgcg
     4921 ctgtgataga gctcgtcctt gccgtgcgcg aggtgcggcg cccacgccgc cttgtcgccg
     4981 aacttgggcg cacccgcgac gccggtgcca tggcagacca cgcaggtgcc ctggaacacc
     5041 ttcttgccgt cggccttggc cgaggcgggt gtcgccttcg ccgccggcgc ggcgggagcc
     5101 ggcgcggtgg gcgcgccgtg atcgtgatca tggcccgccg cgtcggcggc gggagcgggc
     5161 tgcgcctgcg cggtctgcgg cgcgggcgcc gcattctgcg ccggggcggc cggctccttg
     5221 aatccggcac cggcggcgtt cgccatgtac accagcgcgc gctcgatctc gacgtccgag
     5281 agatcggccg cgccgccttt cggcggcatc gcgcccttgc ccttcaaggc ggattgcagc
     5341 agcgccttct cgccgcgctt gatgcgcggc ccccagtcgc cgcggttgcc gaatttcggc
     5401 gcgccctgga tgcccgccgc gtgacacgcc tggcacaccg tcttgtagac ctcgtcgccg
     5461 ctcttcgcgg cctgcgatcc gcgggcggac ccgatcgtca cttcggcgac gggacggatg
     5521 cgctcggcga ctttctcggg cgagagcgcg acgctgtcgg gatcggggcg cggcccgctg
     5581 gtgacgaggt acgcgagcgc gaggatgatc gtgatcggaa cgatgaacgc gagcaggacc
     5641 acgatgacga gctgcttcgg cgtgcggatg ggcgaggtat gcggtggaat atcgggggcg
     5701 gccatggtgt tcgcgggcgg acagtgcaaa agcgcggatt atacccggtg tacaatatcg
     5761 gaccccgcgc ccgtagctca ggggatagag tgttggcctc cgaagccaaa ggtcgcgcgt
     5821 tcgattcgcg ccgggcgcgc cacatttcgg ctcaccgttc cgggaaaccg atgcatcgcg
     5881 gcgtctcgct cattttcgtc cgcgtcgttg ccgtggcact ccttctcgtc acgaccgccg
     5941 cgctcgcggg cggctccagc tacggcatcg cgccgggctc gaggcagatc tccggcaagg
     6001 tctccgaatg gccggtgccc acgccgaagt tcgcgcgcga tcccgcgccc ggccccgacg
     6061 gcaacatcta catcacggtc atgcagggcg accgcatcgc gcgcttcgat ccgcgcaggc
     6121 aggtcttcaa cgagtgggac gtgcccgcgg gcgcgcatcc tcacggcctc gtcgtcgacc
     6181 gcgacggcaa ggtctggtac acgggcaacg gcaacggcac gatcggcgag ctcgatccgg
     6241 cgagcggcaa ggtgacgcag cacaaggtgc cctcgggcgg cagcccgcac acgatcgtga
     6301 tcgccgacga cggcgcgctg tggttcacca accagtcggg ctcggtcggg cggctcgatc
     6361 gcaggagcgg ccgcatcacc gaggtgcgca tgcccggcgg tccctacggc atcgcgctcg
     6421 accggcaggg caacgtctgg gtctgccgca tgaacgccga ccggctcggc cggatcaacc
     6481 cggccaccgg ccaggtatcg gagctcttca tgggctcggg ctcgcgcccg cggcgcatgg
     6541 cggccgcgcc cgacggaacg ctctgggtca cgctgtacgg cacggggcac ctcgcgcacg
     6601 tcgatcccgc cacgtcgaag gtgatccgcg aatacaggat gccggccggc gacgacggcg
     6661 gcccttacgc cgtcaccgtc gacggcgcgg gacgcgtgtt cgcgagcgag atccagtcgg
     6721 acacggtggc gatgctcgat ccgaagacgg agacgttcca ggtctacagg ctgccctcga
     6781 agaacgtcgg catccgcaag gcgatcgtcg acgcgcaggg acgcttctgg tacatgggct
     6841 cgcacaacgg gcggctgggc gtcatcgaat agatcacaaa caagccacga caagcaacgc
     6901 aaccaaggag agagagatgc acgggttcag agcaacgaaa ttcctgatcg ccgccgcggc
     6961 ggcgctctgg gcgggcggcg cgctcgcgca gaattatccg acgcgcgcgg tgaccatgta
     7021 cgtgccgtac gcggccggcg gcccgaccga catcgtcgcg cgcgtggtgg cgcaggccat
     7081 gaccaagccg ctcgggcaga cggtgctggt cgagaaccgg ccgagctcgg gcggcttcct
     7141 cgcgccccag caggtcaagg acgcgaagcc cgacggctat tcgatcctga tccatcacat
     7201 cggcatggcg acgatcccga tcctgtaccg gcagccgaaa ttcgatccgc tcaaggactt
     7261 cgactacatc gggctgatca acgaagtgcc gatgacgatc atcgcgcggc agaatttccc
     7321 ggcgaagaac ttcagggagt tcctctcgta catcaaggcg aacaagagca aggtgacgct
     7381 cgcgaacgcg ggcatcggcg cggcctcgca cctgtgcggg ctgctgttca tgagcgcgat
     7441 ccagaccgat ttcctgaccg tgccgtacaa gggcacgggg ccggcgatga acgacctgct
     7501 cggcggtcag gtcgacttca tgtgcgacca gacgaccaac accacctcgc cgatcaagag
     7561 cggaaaggtg aaggcgtacg cggtgacgac gccgaagcgc ctggcgtcgc tgcccgacat
     7621 cccgacgctc gacgagagcg gcgtcaaggg cgccgacgtc accgtgtggc acgccctcta
     7681 cgccccgaag ggcacgccca agcccgtgat cgacaagctg gtctcggcgc tgcaggccgc
     7741 gctcaaggac aagaccgtga acgagcgctt cgccgagctt ggcgcggtga cggtgtcggt
     7801 cgatcgccag aacccgcagg cgctcgagaa attcctgaag tccgagatcg acaagtgggg
     7861 tcccatcatc cgtaaagcgg ggcagtacgc tgactgaagt ctcataacga aggaggaccg
     7921 aaaatgagga gagtgaacat tgcgctcact gcggcgctcg ccgccgcggc gctcgccggc
     7981 tgcgccggga tgacgccttc gggcggatcg gccggccccg gcgtggtgaa ggtcaccgcg
     8041 ctcggcagcc acgacggcga gttctgctcg cgcgaccgcg cgctcgtgtt cgaggacccg
     8101 gacggcacgc gcatcctgta cgacgccggg ttcacggtgc gcggcgccga cgacccgcgc
     8161 ctcggcagga tcgacgcggt gctgatctcg cacctgcacg gcgaccacct cgggcccgcg
     8221 caccagccgt cggcgaacgc gggcgcgtgc ggcaatccgc agttctcggt gagcgatgtc
     8281 ccgaactcga acacggtcaa catcgtggtc gcgaagcggg ccaagttcct ggtcggcggc
     8341 gacatgaaca cctggttcgc gcacaaggtg aaggccgcgg gcggcgatcc ggcgcaggtg
     8401 cagctcgtgc gcttcggggc gtcggccaag gtcggcggcg tcaaggtgac caccgtgccc
     8461 gccgcgcaca ccaacgcggt cgacccggcg ttcatcgaca agccgctcgc cgacctgctg
     8521 cgcgcgaacg gcctgctggc cgaggtcggg ccgccggtcg gctacgtcgt gagcttctcg
     8581 aacgggctgg tcgtctatct ctcgggcgac accggcgtga tcgcggagca ggacgtggtc
     8641 gtgcggcgct actacaaggc gaacctcgcc gtcatcaaca tcggcgacgt gttcacgacg
     8701 ggtccgagcg aggccgcgta cgtgatcgac gacctggtca agccgagttc ggtgatcgcg
     8761 tcgcacgcga acgaggtcgc gaccaagggc ggcaaggtca tcccgggaac ccggaccgcg
     8821 accttcatga agctcgtgca cgtgccggcg tatgtcccgc tttcgggcca tacgatgtcg
     8881 ttcgacggcg gcggcaagtg cgtggccggc tgctagactg acgccggaag aaggtcggaa
     8941 ggggcgcctt cgggcgccct tttacttttc aggagaaacg atgcgcctgc tgctgctctg
     9001 gctgatcaac gcggtgacgc tgctgctgat cccgtacctc atgccgtcgg tgagcgtgag
     9061 cagcttctac acggcgctga tcaccgcgct gatcctgggg ctggtgaact cggtgatccg
     9121 cccggtcctg gtgctgctca cgctgccgat cacgatcgtg acgctcgggc tgttcatcct
     9181 ggtgatcaac ggcctgatgt tctggttcgt cgcgtcgttc atcgagggat tccacgtcga
     9241 ggggttctgg tcggcgttct ggggtgcgat cgtgtacggg atcctctcga ccgtcttcag
     9301 cgcgatcctg ctgccgcgtc accatccgcc gccgccgccg gtgaggcgcg acgtctaaat
     9361 gaggatgatg tcgaactgct cgggcgtgta gctcgtctcg acctgcatcg acacgggctt
     9421 cccgatgaag tccccgagca tcgccagcgc ctgcgactcc tcctcgagga agaggtcgat
     9481 caccgactgc gacgcgagga tccggtactc gcgcgcgctg aactggcgcg cctcgcgcag
     9541 gatctcgcgc aggatctcgt agcacaccgt ctgcgccgtc ttgacgacgc cgcggcccga
     9601 gcacgtcggg cacggctcgc acaggacgtg ctcgagcgac tcgcgcgtgc gcttgcgcgt
     9661 catctcgacc agtcccaggg cggtgaaccc gttcacggtc atcttggtgc ggtcgcgcgc
     9721 gagcgtcttg ttgaactcct cgagcacggc ggcgcggtgc tcggggttct ccatgtcgat
     9781 gaagtcgatg atgatgatgc cgccgaggtt gcgcagccgc agctggcgcg cgatcacctg
     9841 cgccgcctcg aggttggtct tgaaaatggt gtcgtcgaag ttgcgcacgc cgacgtagcc
     9901 gccggtgttc acgtcgatcg cggtcgtcgc ctcggtctgg tcgatcacga ggtagccgcc
     9961 cgacttgagc tccacgcgcc gcgcgagcgc gtgctcgatc tcgtcctcga cgttgtggag
    10021 atcgaacagc ggccgctcgc cgctgtaatg ttcgacccgc ggcaggacgt tcggcatgta
    10081 ctcgccggcg aacccctgca gcttctggaa gttctcgcgc gagtcgatga cgatgcggcc
    10141 ggtgcgctcg gacacgaaat cgcgcagcac gcgctgcgcg agcgacaggt cctggtacag
    10201 cagcgccggc gccgacgagg tgagcgcccg gccgcggatg tcgtgccaga ggcgccgcag
    10261 gtaggagacg tcggccgcga gctcggcctc ggtcgcggtc tcggcgaccg tgcgcacgat
    10321 gaagccgccg gcttcgtcct cgggcagcag atgctgcagc cttccgcgca gccgctcgcg
    10381 ctcggcctcg tcctcgatgc gctgcgagat gccgatgtgc gggtcctgcg gcaggtagac
    10441 gagcaagcgc cccgcgatcg agatctgcgt cgacaggcgc gcgcccttgg tgccgatcgg
    10501 gtccttgatc acctggacca ggatcgattg cccctcggcg aggatcttct cgatcggctt
    10561 cgccgccgcc gggctctgct gcgcggcgcg ccggtgctcc cagatgtcgg ccacgtgcag
    10621 gaaggccgcg cgctccaggc cgatctcgac gaacgccgat tgcatgccgg gcagcacgcg
    10681 cgcgatcttg ccggcgtaga tgttgccgac gatgccgcgc gcgagcgcgc gctcgacgtg
    10741 cagctcctgt accacgccgc tctgcatcac ggcgacgcgg gtctcctgcg gcgtgcagtt
    10801 gatgaggatg tcctcgatca ccgcacgccc gcttctatcc gccttccgcg cggaaggtct
    10861 cgatcagctg ggcagtctcg tagagcggca ggcccatgac gcccgagtag ctgccgcgca
    10921 gctcgggcac gaaggcggcg gcgcggccct ggatggcgta cgcgccggcc ttgtccatcg
    10981 gctcgccggt gtcgatgtag gactggatgt cggcgtccga tatcttgcgg aagcgcacca
    11041 cggtctcgtt gaccgtcatc ttcgagcgcg attcgtagcg gatggcgacc gcggtgagca
    11101 cgcgatgctc gcgtcccgag agcagcgcga gaatgcgcgc cgcgtcggcg cggtcgaccg
    11161 gcttgccgag gatctgctcg tccacgcaca cggtggtgtc ggcggcgagc acgggctggc
    11221 gcggcagctg ccgctgcacc acgcggtccc agccgacgtc ggccttgagc ttgccgatgc
    11281 ggcgcacgta gtcgtcgggc ttctcgctcg gcagcgcggc ctcgttcacg tcgggcccgc
    11341 gcgcaggctg ctcgcgaagc aggatgacct cgaaggcgat gccgatctgc ttgagcagct
    11401 cgcggcgtcg cgggctgcgc gaggcgaggt agaacttggg ggctatggcg gggggtttcg
    11461 cgggcgtggc catggggctc cgaggtcgtg gcttcttgta gttaatcgca aagcgatggt
    11521 agacgcaaat gtgcggacga actcggcgct cactcccggt ggtacgggtg cccggcgagc
    11581 agcgacgccg cgcggtagag ctgctcggcg aggacgaccc gggccagccc gtgcggcagc
    11641 gtcagcgacg acaggcgcat cgaagccgcg ccgcgcttga tctcggcggc gagcccgtcc
    11701 ggtccgccga tcaggaaggc gaggtcgtcg ccttcgccgc ggcgctcctc gagccagcgc
    11761 gcgaactccg ccgtcgtgta gtcgcggccg cgctcgtcga ggacgacgcg ccgcgcgcgc
    11821 gcgggcagcg cccgctcgat gcgcgcggcc tcggcggcga ggatctgcgc cggcgtgcgt
    11881 cccgcgcttc gcggctcggg acgcacttcc acgagctcga gcgccagcgt ccgcggcatg
    11941 cgccgggcgt attcggcgaa ggcttcgtcg acccaccgcg gcatgcgagc gcccacggcg
    12001 acggcgagca gcttcacgcc ggctttcgcg ccttgcgggt tccggcggga cgtttggcgc
    12061 gcgccgcggt tcctcgtgcg ggccgcggct tgggctggtc ccacagctcc tcgaggttgt
    12121 aatggacgcg cacggccggc tgcatgatgt gcacgacgat gtcgccgagg tcgacgagga
    12181 cccattcgcc gctctcctcg ccttcgacgc cgacaatgcg gccgcccgcc gccttcgtct
    12241 tctcgcgcac gctgttcgcg agcgcgcgca gctgccggtt cgactcggcg ctcgcgatga
    12301 ccacgcggtc gaacagcgac gagaggtgcg ccacgtcgta cacgcggatg tccttcgcct
    12361 tgacatcctc gagggcgtcg acgacggcgg cctcgagctt gcggatgggc gtcacgcgcg
    12421 attcccggta tagatgccgt gcgactcgat gtactcgagc accgcggccg ggagcaggta
    12481 acggggaagc agcccctggg cgaagtggcg ccggatggcg gtcgccgaga tgtcgagggc
    12541 ggtggtggcg tagatgacga cgcggcccgc ggggcgcgcg acgatctcct cgggcgtgcc
    12601 cgagcgctcg cgccactgcg cggcgagctc ggcgggcatg ccgcccggcg tcagcgaatt
    12661 ccccggtcga agtccgacga agatatgcgc cagctcgaac aggtcgcgcc agcgctccca
    12721 cgtggtgaga ttgcggaacg aatcggtgcc gagcagcagc acgatggagc gcttttcgcc
    12781 cagctcgcgg cgcagccgct ccagcgtctc gaccgtgtag ctcggcgtgg tctggcggat
    12841 ctcggcgtca tcgagctcga agctcggatg acgcgcgatc gcgagcttga ccatctccag
    12901 gcggtccgcc gcggcggcca tcggcgggtt ccggtgcgag ggctggcccg cggggatcca
    12961 gcgcacgcat tcgagccgga ggatctcgac cgcctcgtcg gcgagccgca ggtggccgaa
    13021 atgaaccggg tcgaacgtgc cccccatgat gccgacggga gccgcaccgc cccgagctgg
    13081 cttgcgcccg ggcatgtcag ccgcggacct ggcccgagcc gtacaccacg aacttctgcg
    13141 aagtgaggcc ttcgaggccg acggggccgc gcgcgtgcag cttgtcggtc gagatgccga
    13201 tctcggcgcc gaggccgtac tcgaagccgt cggcgaagcg ggtcgacgcg ttgacgagga
    13261 ccgagctcga gtcgacctcg cgcaggaagc gctcggcgcg cgcgcggtcc tcggtgacga
    13321 tcgcgtcggt gtgctgcgag ccgtaccggg cgatgtgcgc gatcgcctcg tcgaggccgc
    13381 gcaccacccg caccgcgagg atcggcgcga ggtactcggc gtaccagtcc tcctcggtcg
    13441 ccgccttcat gagcgggacg agcgcgcgcg cgcgctcgtc gccgcgcagc tgcacgccct
    13501 tgtcgaagta gatcttggcg agctttggca ggaccttgtc cgccacgcct tcggccacga
    13561 gcagcgtctc catcgcgttg cacacgccgt agcgctgcgt cttggcgttg tcggcgatgc
    13621 ggatcgcctg gtcgatatcg gcgcggtcgt cgatgtagac gtggcacacg ccgtcgaggt
    13681 gcttgatcgt cgggatgcgc gactcgcgca tcacgcgctc gatcaggctc ttgccgccgc
    13741 gcggcacgat gatgtcgacg tgctcgttca tcgagagcag gatgccgacc gcggcgcggt
    13801 ccggcgtgtc gacgacctgg accgcgttct cgggcagccc gcccgcgcgc aggccctcgt
    13861 gcatgcacgc cgcgatcgcg agattcgagc gcaccgactc cgagccgccg cgcaggatgc
    13921 aggcgttgcc cgatttgagg cacagcgccg cggcgtccgc ggtcacgttg ggccgcgact
    13981 cgtagatgat gccgatcacc ccgagcggca cgcgcatgcg cccgaccttg atgcccgagg
    14041 gccgctcgcg cagctcgctc acctcgccga ccggatcggc gagcttcgcc acctcctcca
    14101 ggcccgcggc catggcctcg acggatttcg gggtgagcgt caggcgatc
//
LOCUS       DATDMW010000244         6558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_75654, whole genome shotgun sequence.
ACCESSION   DATDMW010000244 DATDMW010000000
VERSION     DATDMW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75654"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /gene="ftsH"
                     /locus_tag="VI319_10495"
     CDS             <1..1326
                     /gene="ftsH"
                     /locus_tag="VI319_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY6864317.1"
                     /translation="GRIPKGVLMVGNPGTGKTLLARAIAGEAKVPFFSISGSDFVEMF
                     VGVGAARVRDMFDQAKKHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMD
                     GFEGTTGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILLVHMRKVPLA
                     PDVRAEIIARGTPGFSGADLANLVNEAALFAARKNKRLVDMDDFERAKDKIVMGAERR
                     SMVMPEEERRNTAYHESGHAVVARLLPKTDPVHKVTIIPRGRALGVTMQLPEEDRYSM
                     DRERLLSTIAVLFGGRIAEEIFMNQMTTGASNDFERATAIARSMVTQWGMSEAMGTMV
                     YGENEGEVFLGRSITTHKNVSEATLQRVDGEIRKIIDGQYGLARKLILDNREKVEAMA
                     KALLEWETIDADQIGDIMEGRPPRPPKPAHSPQQPTQPGAPGAPEPAAA"
     gene            1364..2215
                     /gene="folP"
                     /locus_tag="VI319_10500"
     CDS             1364..2215
                     /gene="folP"
                     /locus_tag="VI319_10500"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY6864318.1"
                     /translation="MAHAVLACGRFRLDLGRPLVMGVVNVTPDSFSDGGLYLDPAKAI
                     AHARRLIEEGADLLDVGGESSRPGAQGVPADEELRRIRPVLRDLRDAPVPVAVDTVKP
                     DVMRAALDEGAAMINDIGALRAPGAMEVVAPSDAGVCLMHMQGEPRTMQESPSYRDVA
                     AEVRAFLGQRAAAAEAAGIARERIVIDPGFGFGKKPAHNLELLRRLDEIASLGYPVLA
                     GLSRKSTLGSITGRPPVERAAASVAAALLAVEAGARIVRVHDVAATKDALAVWSAVRA
                     GVPGNAT"
     gene            2205..3575
                     /gene="glmM"
                     /locus_tag="VI319_10505"
     CDS             2205..3575
                     /gene="glmM"
                     /locus_tag="VI319_10505"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY6864319.1"
                     /translation="MPRRYFGTDGVRGRVGQSPITPEFVMRLGYAAGDVLVRRARLRG
                     SGLSADQHPVVLIGKDTRVSGYMLEASLEAGFSAAGVDVMLTGPLPTPAIAYLTRALR
                     LQAGIVISASHNPYEDNGIKFFGSDGSKLPDEVESEIELKLENPLQCKESAELGKAQR
                     IDDAAGRYIEFCKSTFPNRLDLRGLRIVLDCAHGATYHVAPPVFHELGAEVSVIGASP
                     NGFNINDGFGATQPDALREAVRAQRADLGIAFDGDGDRVLMIDQEGRTYDGDQLLYVI
                     ASERSRGGPVPGVVGTLMTNLALELALEKMGIAFARAKVGDRYVLETMLERKWQLGGE
                     NSGHIICLDKHTTGDGIVSALQVLSALRTSGSNLDKLCAGLTLFPQVMVNVPVKNGFD
                     WQKESAIVQAKNDAERALGKDGRILLRASGTEPVLRVMVEGKDETRVRTLARDIARVV
                     ETAAGS"
     gene            3572..4039
                     /gene="dtd"
                     /locus_tag="VI319_10510"
     CDS             3572..4039
                     /gene="dtd"
                     /locus_tag="VI319_10510"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY6864320.1"
                     /translation="MIGLLQRVVEARVDVGDERVGSIERGILVFVCAEPGDTESTADR
                     LLERLLGYRVFPDAAGKSNLSVQDVAGGVLLVPQFTLAADTAKGTRPSFSSAADPRRA
                     QVLFDHLVRRARSAHASVETGRFGAHMRVALTNDGPVTFWLRVAPPAEPDGKH"
     gene            4118..4870
                     /gene="tpiA"
                     /locus_tag="VI319_10515"
     CDS             4118..4870
                     /gene="tpiA"
                     /locus_tag="VI319_10515"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY6864321.1"
                     /translation="MRARLVVGNWKMHGNLATNQALLDAVRGLPGDERVAYAVCPPFP
                     YLQQAAGLLAGSAVRWGGQDLCEQDLGAYTGEVSGAMLRDLGCRYVIVGHSERRALYA
                     ETDERVAAKFEAARRAGLTPILCVGETLAERDSGQTETVLARQLKAVLDRAGVKALTE
                     SVLAYEPVWAIGTGRTASGDQAQSAHAFLRSMVRKQDRAIGDALTILYGGSVKPANAA
                     ELFGMPDVDGGLIGGASLVAADFVAICKAARH"
     gene            4913..5287
                     /gene="secG"
                     /locus_tag="VI319_10520"
     CDS             4913..5287
                     /gene="secG"
                     /locus_tag="VI319_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY6864322.1"
                     /translation="MDFWFTLILIIDVLVAMGIIGLVLLQHGKGADVGAAFGSGASGS
                     LFGASGSANFLSRSTAVLAVVFFITTFTLAYLASHRPRGESAVIDSVKEQPAAPPAPA
                     TGGDNAPKAPTTPDPKANEVPK"
     gene            5331..5415
                     /locus_tag="VI319_10525"
     tRNA            5331..5415
                     /locus_tag="VI319_10525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5365..5367,aa:Leu,seq:gag)
     gene            5437..5796
                     /locus_tag="VI319_10530"
     CDS             5437..5796
                     /locus_tag="VI319_10530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY6864323.1"
                     /translation="MVLENYFPILLFIVVALIFGGVLVGAGTLLAPNRPDADKLSPYE
                     CGFEAFEDARMKFDVRYYLIAILFILFDLEIAFLFPWAVTLNDIGSFGFWSMMVFLAI
                     LVVGFIYEWMKGALEWE"
     gene            5787..6263
                     /locus_tag="VI319_10535"
     CDS             5787..6263
                     /locus_tag="VI319_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY6864324.1"
                     /translation="MGIEGVMQEGFVTTSIDKVINWTRTGSMWPMTFGLACCAVEMMH
                     AGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIVQLQNKIKRTNTIAR"
     gene            6260..>6558
                     /locus_tag="VI319_10540"
     CDS             6260..>6558
                     /locus_tag="VI319_10540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY6864325.1"
                     /translation="MRSKLDELKTALGAALGERAAALTESLGELTLVVKAADYLPTAQ
                     TLRDDPSLRFEQLVDLCGVDYSAFAETARDGSRFGVVSHLLSVTRNRRLRLRVFA"
BASE COUNT         1038 a         2164 c         2328 g         1028 t
ORIGIN      
        1 ggccgcatcc ccaagggcgt cctcatggtc ggcaacccgg ggaccggcaa gacgctgctc
       61 gcgcgcgcga tcgcgggcga ggccaaggtg ccgttcttct ccatttccgg atccgatttc
      121 gtcgagatgt tcgtcggtgt cggcgcggcg cgcgttcgcg acatgttcga ccaggcgaag
      181 aagcacgcgc cttgcatcgt gttcatcgac gagatcgacg cggtggggcg ccagcggggc
      241 gccggcctcg gcggcggcaa cgacgagcgc gagcagacgc tgaaccagct gctcgtcgag
      301 atggacggct tcgagggcac gaccggcgtg atcgtcatcg ccgcgaccaa ccggcccgac
      361 gtgctcgacc cggcgctgct gcgcccgggc cgcttcgacc gccaggtcgt cgtgccgctg
      421 cccgacatcc gcggccgcga gcagatcctg ctcgtgcaca tgcgcaaggt gccgctcgcg
      481 cccgacgtcc gggcggagat catcgcgcgc ggcacgccgg ggttctccgg cgccgacctc
      541 gccaacctgg tgaacgaggc ggcgctgttc gccgcgcgca agaacaagcg cctcgtcgac
      601 atggacgact tcgagcgcgc caaggacaag atcgtgatgg gcgccgagcg ccgctcgatg
      661 gtcatgcccg aggaggagcg caggaacacg gcgtaccacg agtcgggtca cgcggtggtg
      721 gcgcgcctgc tgcccaagac cgacccggtg cacaaggtga cgatcatccc gcgcggacgc
      781 gcgctcggcg tcaccatgca gcttccggaa gaggaccgct acagcatgga ccgcgagcgg
      841 ctgctctcga ccatcgcggt gctcttcggc ggccgcatcg ccgaggagat cttcatgaat
      901 cagatgacga ccggcgcctc gaacgacttc gagcgcgcga ccgcgatcgc ccgcagcatg
      961 gtcacgcagt ggggcatgtc cgaggcgatg ggtaccatgg tctatggcga gaacgagggt
     1021 gaggtcttcc tcgggcgctc gatcaccacg cacaagaacg tctccgaggc gacgctgcag
     1081 cgcgtcgacg gcgagatccg gaagatcatc gacgggcagt acggcctcgc gcgcaagctg
     1141 atcctcgaca atcgcgagaa ggtcgaggcg atggcgaagg cactgctcga gtgggagacc
     1201 atcgacgccg accagatcgg cgacatcatg gagggccggc cgccgcggcc gcccaagccc
     1261 gcgcactcgc cgcagcagcc gacgcagccc ggggcgcccg gggcgccgga gccggcggcg
     1321 gcataacacc caggcccgaa gcgcgcttcg ggcctttttc tttatggcgc acgccgttct
     1381 cgcttgcgga cgcttccggc tggatctcgg gcgcccgctc gtcatgggcg tggtcaatgt
     1441 gaccccggat tcgttctccg acggcggcct gtatctcgat cctgccaagg caatcgcgca
     1501 cgcccgccgg ctgatcgagg agggcgccga cctgctcgac gtcggcggcg agtcgagccg
     1561 gccgggagcg cagggcgtgc ccgccgacga ggagctgcgc cgcatccggc ccgtgctgcg
     1621 cgatctgcgc gacgcacccg tgccggtcgc cgtcgacacg gtgaagcccg atgtgatgcg
     1681 cgccgcgctc gacgaaggcg ccgccatgat caacgacatc ggcgccctgc gcgcgcccgg
     1741 cgcgatggaa gtcgtggcgc cgagcgatgc gggcgtttgc ctcatgcaca tgcagggcga
     1801 gccgcgcacg atgcaggaga gtccgagcta tcgggatgtc gccgccgagg tgcgcgcctt
     1861 cctcgggcag cgcgccgccg cggccgaggc ggcaggcatc gcccgcgagc gcatcgtgat
     1921 cgacccgggc ttcggcttcg gcaagaagcc cgcgcacaat ctcgagctgc tgcggcgtct
     1981 ggacgagatc gcgtcgctcg gctacccggt gctcgcagga ctgtcgcgca agtcgacgct
     2041 gggttcgatc accggccggc cgccggtcga gcgcgcggcg gcgagcgtcg cggcggcttt
     2101 gctggccgtc gaggccgggg ctagaatcgt gcgcgtccac gacgtagccg caaccaagga
     2161 cgcgctcgca gtctggagcg ccgtccgcgc aggagttccc ggcaatgcca cgtagatact
     2221 tcggtaccga cggcgtcagg ggtcgcgtcg gccagtcgcc gattaccccc gagttcgtga
     2281 tgcgcctggg ctacgcggcc ggcgacgtgc tggtgcgacg cgcccggctg cgcggctcgg
     2341 gcctctcggc ggaccagcat cccgtggtgc tcatcggcaa ggacacgcgc gtctcgggct
     2401 acatgctcga ggcgtcgctc gaggcgggat tctcggcggc gggggtcgac gtgatgctca
     2461 cgggccccct gccgacgccc gcgatcgcct acctgacgcg tgcgctacgg ctgcaggcgg
     2521 ggatcgtgat cagcgcctcg cacaatccct acgaggacaa cggcatcaag ttcttcggct
     2581 ccgacggcag caagctgccc gacgaggtgg aatcggagat cgagctgaag ctcgagaacc
     2641 cgctgcaatg caaggaatcc gccgaactgg gcaaggcgca gcggatagac gacgccgcgg
     2701 ggcgctacat cgagttctgc aagagcacgt tcccgaaccg gctcgatctg cgcggcctca
     2761 ggatcgtcct cgactgcgcg cacggcgcca cctaccatgt cgcgccgccc gtgttccacg
     2821 agctcggcgc cgaggtgagc gtcatcggcg cgtcgcccaa cggcttcaac atcaacgacg
     2881 gcttcggggc gacgcagccc gacgcgctgc gcgaggcggt acgcgctcag cgcgccgatc
     2941 tcgggatcgc cttcgacggt gatggcgacc gcgtgctgat gatcgatcaa gagggacgaa
     3001 cctacgacgg cgaccagctg ctctacgtca tcgccagcga gcgttcgcgc ggcgggccgg
     3061 ttcccggggt cgtgggcacg ctgatgacga acctggcgct ggagctggcg ctggaaaaga
     3121 tgggtatcgc cttcgcgcgc gcgaaggtcg gggaccgcta cgtgctcgag acgatgctcg
     3181 agaggaaatg gcagttgggc ggcgagaact ccggccacat catctgcctc gacaagcata
     3241 cgacgggcga cggcatcgtg tcggcgctgc aggtgctttc ggcgctccgg acgagcgggt
     3301 cgaacctcga taagctctgc gcgggcctga cccttttccc gcaggtgatg gtcaacgtac
     3361 cggtgaagaa cggcttcgac tggcagaagg agtcggccat cgtccaggcg aagaacgacg
     3421 ccgagcgcgc gctcggcaag gatggtcgca tcctgctgcg cgcgtcggga accgagccgg
     3481 tgctccgcgt catggtcgag ggcaaggacg agacgcgcgt gcgtacgctc gcgcgcgaca
     3541 tcgccagggt ggtcgagacc gctgcgggct catgatcggc ctcctgcagc gcgtcgtcga
     3601 ggcacgggtc gatgtcggcg acgagcgtgt cgggtccatc gagcgcggga tactcgtctt
     3661 cgtttgcgcc gagcctgggg acaccgagtc tacggccgac cggctgctcg agcgcctgct
     3721 cggctatcgc gtgttcccgg acgccgcggg gaagtcgaac ctctcggtgc aggacgtcgc
     3781 cggcggggtg ctgctcgttc cgcagttcac gctggccgcc gataccgcca agggtacgcg
     3841 cccgagtttt tcctcggccg ccgacccgag gcgcgcccag gtcctgttcg atcatctggt
     3901 ccggcgcgcg cgttccgcgc acgcgagcgt ggagaccggg cgcttcggag cgcacatgcg
     3961 ggtcgcgctc acgaacgacg gtccggtgac gttctggttg cgcgtcgcgc cgcccgccga
     4021 gcccgatggg aagcactgac aagactctga atttcccggt attttcgttg acccgggccg
     4081 tcggcgccga taaaatcacg cgtttcggat cgatcgaatg cgagccaggc tcgtagtggg
     4141 caactggaag atgcacggca acctggcgac gaaccaggcc ctgctcgatg cggtgcgcgg
     4201 gctgcccggc gacgagcgcg tcgcgtacgc ggtttgcccg cctttcccgt atctgcagca
     4261 ggcggccggc ctgctcgccg gcagcgcggt ccgctggggc gggcaggatc tgtgcgagca
     4321 ggacttgggc gcgtatacgg gcgaagtgtc gggcgcgatg ctgagagacc tgggatgccg
     4381 ctacgtgatc gtcggccact ccgagcggcg cgcgctgtac gcggaaacgg acgagcgcgt
     4441 ggccgccaag ttcgaggccg cgcggcgcgc cgggctgacg ccgatcctgt gcgtgggcga
     4501 gacgctcgcg gagcgcgata gcggtcagac cgagaccgtc ctcgcgcggc aactcaaggc
     4561 ggtgctcgac cgcgccggcg tcaaggcgct taccgagtcg gtgctcgcct acgagccggt
     4621 gtgggcgatc gggacgggca ggacggcgag cggggaccag gcgcagtcgg cgcacgcgtt
     4681 cctgcgcagc atggtgcgaa agcaggaccg ggcgatcggc gacgcgctga cgatcctgta
     4741 cggcgggagc gtgaagccgg cgaacgcggc ggagctgttc gggatgcccg acgtggacgg
     4801 cggcctgatc ggcggcgctt cgctcgtggc tgcggatttc gtcgcgatct gcaaggcggc
     4861 ccggcattga atctcgagcg gcgaatcgcc gcataggcaa ggaagggacg gcatggactt
     4921 ctggtttact ctgatcctga tcatcgacgt gctcgtggcg atgggcatca tcgggctcgt
     4981 gctcctgcag cacggcaagg gcgccgacgt gggcgcggcc ttcggcagcg gcgcctcggg
     5041 cagcctgttc ggcgcgagcg gctcggccaa cttcctctcg cgcagcacgg cggtcctcgc
     5101 cgtcgtgttc ttcatcacca cgttcacgct cgcctacctc gcgtcgcatc gtccgcgcgg
     5161 cgagagcgcc gtgatcgatt cggtgaaaga gcagccggcg gcgccgccgg ccccggcaac
     5221 gggtggcgac aacgcgccca aggcgcccac gacgccggac ccgaaggcaa acgaggtgcc
     5281 gaagtagtaa aatcgaaagc ttgagaggcc ggcctccgcc tcgatgcaat gccgacgtgg
     5341 tgaaattggt agacacgccg tcttgagggg gcggtggcga aagccgtgag agttcgagtc
     5401 tctccgtcgg caccataacg cgggcaagcc caacgaatcg tgctcgagaa ttactttccg
     5461 atcctcctct tcatcgtcgt cgcgctgatc ttcggcggcg tgctggtcgg cgcgggaact
     5521 ctgctggcgc cgaaccgtcc ggacgccgac aagctttccc cctacgagtg cggcttcgag
     5581 gcgttcgaag acgcgcgcat gaagttcgac gtgcgctact acctgatcgc gatcctgttc
     5641 atcctgttcg acctggagat cgcgttcctg tttccgtggg cggtcacgct caacgacatc
     5701 ggcagcttcg gattctggtc gatgatggtg ttcctggcga tcctcgtggt cggcttcatc
     5761 tacgagtgga tgaagggggc cctcgaatgg gaatagaagg cgtcatgcag gagggattcg
     5821 tcaccacctc catcgacaag gtcatcaact ggacccgtac cggctcgatg tggccgatga
     5881 ctttcgggct cgcgtgctgc gcggtcgaga tgatgcacgc gggcgccgcg cgctacgacc
     5941 tcgatcgctt cggcgtggtc ttccgcccga gcccgagaca gtccgacgtg atgatcgttg
     6001 cgggaacgct gtgcaacaag atggcgcccg cgctgcgcaa ggtctacgac cagatggccg
     6061 agccgcgctg ggtgatctcc atgggatcgt gcgcgaacgg cggcggctac taccactatt
     6121 cgtattccgt cgtacgcggc tgcgaccgca tcgtgccggt cgacatctac gtgccggggt
     6181 gcccgccgac cgcggaggcg ctgctgtacg ggatcgtgca gctgcagaac aagatcaagc
     6241 gtacgaacac gatcgctcga taaggtccaa gctggatgag ctgaagaccg ccctgggtgc
     6301 cgcgctcggg gagcgggccg ccgcgctgac cgaatcgctc ggcgagctga cgctcgtggt
     6361 gaaggcggcc gactacctgc cgacggccca gacgctgcgc gacgatccgt cgctgcgctt
     6421 cgagcagctc gtcgatcttt gcggggtcga ctattccgcc ttcgccgaga ctgcccggga
     6481 cgggagccgc tttggggtcg tctcgcacct gctgtcggtc acgcggaacc ggcgcctgcg
     6541 gctgcgcgtg ttcgccaa
//
LOCUS       DATDMW010000245         8602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1319016, whole genome shotgun sequence.
ACCESSION   DATDMW010000245 DATDMW010000000
VERSION     DATDMW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319016"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..622)
                     /locus_tag="VI319_10545"
     CDS             complement(155..622)
                     /locus_tag="VI319_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY6864326.1"
                     /translation="MARLLVLLAALACAPAAWPKEAAPAAAEPALEARVMALSSELRC
                     LVCQNQTIADSNAGLASDLREEIREMMRQGKSDRQIVAFMVDRYGDFVLYRPPLKAAT
                     VLLWFGPLLLVLIGIIALFRRLARRHRAEEPPLAPADAERARRLLDGGGTEAR"
     gene            complement(615..1142)
                     /locus_tag="VI319_10550"
     CDS             complement(615..1142)
                     /locus_tag="VI319_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY6864327.1"
                     /translation="MNRFVLPLGIFAVLVALLGIGLTLNPREVPSPLIGKPAPHFDLP
                     QLGEPGKTFSERDMLGKVWLLNVWASWCVSCREEHPVLLDLAKSGAVPILGLNYKDER
                     KDGLSWLQNMGDPYRLSAFDFQGRVGIDYGVYGVPETYLIDKRGVIRYKRIGPVTREV
                     LKEQILPLVQELERG"
     gene            complement(1162..3144)
                     /locus_tag="VI319_10555"
     CDS             complement(1162..3144)
                     /locus_tag="VI319_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY6864328.1"
                     /translation="MNPEIGQLALALALCLALCQSALGLAGAARGNVTWMRAVRPVAV
                     GQFVFVATAFVCLAWAFVANDFSVLYVASNSNSSLPLHYRITAVWGGHEGSILLWTLM
                     LTVWSVAVAAFSRRLPDEMIARVLGVMGLVSVGFLLFMLFTSNPFLRLFPAPADGRDL
                     NPLLQDPGMVIHPPMLYMGYVGFSVAFSFAVAALLGGRLDAAWARWSRPWTTVAWSFL
                     TVGIALGSGWAYSELGWGGWWFWDPVENASFMPWLVGTALVHSLAVTEKRGSFRSWTV
                     LLALVAFALSLLGTFLVRSGVLTSVHAFATDPKRGIFILALLVLVIGGSLLLYVWRAP
                     KIGLGGSFDTVSRESFLLLNNVMLVAAAGSVLLGTLYPLALDAMGLGKISVGPPYFET
                     VFVPLMAPALFLMGIGPLARWKKAELPELGKRLRWAFGASVIAALLLPFALGRWSALV
                     ALGLALAAWIAITSVLQLWQRTAHAAGAQAAWTRLKSTPRAHYGMLLAHFGVAVFVVG
                     VTLVKGYESEQDVRMEPGDTATVGGYLFRFDGVRELQGPNYIAARASIRVERDGKPVT
                     TLYPEKRLYTVQNMPMTEAAIQPGPTRDLYVSLGDQVGATGWIVRAQVKPFVDWIWGG
                     CLLMALGGVLAASDRRYRLAARKEREERAAAAAATA"
     gene            complement(3141..3608)
                     /gene="ccmE"
                     /locus_tag="VI319_10560"
     CDS             complement(3141..3608)
                     /gene="ccmE"
                     /locus_tag="VI319_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY6864329.1"
                     /translation="MKPRHKRLAMIAGGLAALAVAAAFVLSAFRENLVFFFTPTQIAA
                     NEVPHGRAFRIGGLVEAGSLKRLPDGVSVQFVVTDTAKSVKVVYRGILPDLFKEGKGV
                     VTQGRLGPDGTFVASEVLAKHDENYMPPAAADAVAKAQAQAEKTLVVPAGGSK"
     gene            complement(3605..3766)
                     /gene="ccmD"
                     /locus_tag="VI319_10565"
     CDS             complement(3605..3766)
                     /gene="ccmD"
                     /locus_tag="VI319_10565"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY6864330.1"
                     /translation="MNWGSLDNFLAMGGYGLYVWGSYGVTLALLVAEIVLLARRRRSL
                     AERQSPRMR"
     gene            complement(3763..4497)
                     /gene="ccmC"
                     /locus_tag="VI319_10570"
     CDS             complement(3763..4497)
                     /gene="ccmC"
                     /locus_tag="VI319_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEY6864331.1"
                     /translation="MNWYKYSSPQTFFPLAGALAKAFGALAVVACAIGLYIGFFVAPT
                     DAQQGEAYRIIFIHVAAAWMSMFLYVVMAFWAGLGLALNTRLSAMMARAIAPTGALFT
                     FIALWTGSLWGRPTWGTWWVWDARLTSELILLFLYFGFMALQASIDDVRRADKAGAVL
                     ALVGVVNVPIIYFSVKWWNTLHQGASVSLTKAPSMAATMLAGMLAMALGFWFYSIAAA
                     MTRARCIMLERERSTEWATEYLGERA"
     gene            complement(4542..5210)
                     /gene="ccmB"
                     /locus_tag="VI319_10575"
     CDS             complement(4542..5210)
                     /gene="ccmB"
                     /locus_tag="VI319_10575"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY6864332.1"
                     /translation="MLAAVRCILRRDLLLAARRRSDVFTVVLFFIIVASLFPLGVGPD
                     PALLRMIAPGVIWVAALLASMLALHRVFAADHADGTLEQMLLGATPLGVTVAAKVAAH
                     WLASGLPLVLIAPLLALQFDLDQKSLGTLVVSLALGTPVLSLIGAIGAALTLGLRGGG
                     ALLSLLVLPLYVPVLIIGAGAAEAAGSGLGGEAQLLLLAAFLVAAGAFAPWATAVALR
                     ISVE"
     gene            complement(5213..5866)
                     /gene="ccmA"
                     /locus_tag="VI319_10580"
     CDS             complement(5213..5866)
                     /gene="ccmA"
                     /locus_tag="VI319_10580"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEY6864333.1"
                     /translation="MSLLTDGAALEATALRCTRGERTLFSDLSFTLRGGDLLRIAGAN
                     GSGKTSLLRIACGLLEANGGAVRWRGEDVRRLREDYSRELVYLGHAPAVKDDLTAREN
                     LEIACALGGIAAARVDVRGALASLGLAGCEELPARVLSQGQRRRVTLARLVLARAIPL
                     WMLDEPFTALDPAATDRVRALIAEHVARGGSVLYTTHHEVPIAAGRASRIDLGGGRP"
     gene            complement(5873..6775)
                     /locus_tag="VI319_10585"
     CDS             complement(5873..6775)
                     /locus_tag="VI319_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6864334.1"
                     /translation="MPQSIDDTQSLLASADYVAERSLATAVFLSLKMRRPLFLEGEAG
                     VGKTEIAKVLAATLGRRLVRLQCYEGLDVASAVYEWNYSRQMIEIRLAEAEGVKSREG
                     ISQDIFSEKFLIRRPLLQALEGDTGAAPVLLIDELDRTDEPFEAYLLEVLSDFQVTIP
                     EIGTIKAAEPPIVVMTSNRTREIHDAIKRRCFYHWVDYPDAAREIEILKRKAPEAAEK
                     LSREVVAFVQRLRKMDLFKLPGVAETIDWSKALVALDKLALDAQTVNDTLGTLLKYQD
                     DIGRIRGSEAERLLAEVRAELAAA"
     gene            complement(6961..7758)
                     /locus_tag="VI319_10590"
     CDS             complement(6961..7758)
                     /locus_tag="VI319_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY6864335.1"
                     /translation="MYTFNYQKAKSVADAAAVLAKNGDAKPLAGGQSLIAAMKLRLAR
                     PSDVIDLGTLPDLKGIKVDGSSVTIGAMTRHAEVAGSAEVRKAIPALASLAASIGDRQ
                     VRNMGTIGGSLANNDPAADYPAGVLGLGATVVTNKRKIAADDFFKGMYETALEQGELI
                     TSVVFPIPKRAAYMKFKNPASRFALVGVLVADTGKGARVAVTGAAPSVFRVPEMEKAL
                     TSKFAPESVANVKVKPDGLNSDLHAKADYRAHLVTVMARRAVESALK"
     gene            complement(7768..>8602)
                     /locus_tag="VI319_10595"
     CDS             complement(7768..>8602)
                     /locus_tag="VI319_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6864336.1"
                     /translation="ETTFAQVVADGLGLGIEQVEIVHGDTSKILFGMGTYGSRSIAVG
                     GTAIVKALDKIIAKGKKIAAHLLEASEADIEYDRGEFKVAGTDKKKMFGEVAFAAYVP
                     HNYPLDKLEPGLNENAFYDPTNFTFPAGSYVCEVEVDRETGASQIVAFTAVDDFGKVI
                     NPMIVEGQVHGGLAQGIGQALTENCYYDPESGQLVTASYLDYCLPRASDVPSFKVGTE
                     QTPCTHNPLGVKGCGEAGAIGAPAAVMNAITDALKVKDIEMPATPQRIWRTLQQNRKA
                     A"
BASE COUNT         1387 a         2985 c         3024 g         1206 t
ORIGIN      
        1 aggtcggcct cgagctcgcg cagctggtcg cggtagatgt cgacattcgc ctcggtgcgc
       61 gtgcctcgcg aggccgcggg ccggcgcagc agcggcgcgg cgacgaacgc gatcgcgatg
      121 gcgatcagcg ccgccgccgc cgcgacgaac gcgctcatcg cgcctccgtt ccgccgccgt
      181 cgagaagccg gcgcgcgcgc tccgcgtcgg cgggcgcgag cggcggctcc tccgcgcggt
      241 gacggcgcgc gagccggcgg aagagggcga tgatcccgat caggacgagc agcagcggac
      301 cgaaccagag caggacggtg gccgccttga gcggcggccg gtacagcacg aagtcgccgt
      361 agcgatccac catgaacgcg acgatctgcc ggtcgctctt gccctggcgc atcatctcgc
      421 ggatctcctc gcgcaggtcg ctcgcgaggc cggcgttgga atcggcgatc gtctggttct
      481 ggcacacgag gcagcgcagc tcggaagaga gcgccatgac gcgcgcctcg agcgcgggct
      541 ccgcggcggc gggcgccgcc tccttcggcc aggcggccgg cgcgcacgca agcgcggcga
      601 gcagcacgag cagcctagcc acgctcgagc tcctgcacga gcggcaggat ctgctccttc
      661 aggacctcgc gcgtcacggg accgatgcgc ttgtagcgga tgacgccgcg cttgtcgatc
      721 aggtaggtct ccggcacgcc gtagacgccg tagtcgatgc cgacgcggcc ctggaagtcg
      781 aacgccgaca gccggtaggg atcgcccatg ttctgcagcc aggagaggcc gtccttacgc
      841 tcgtccttgt agttcaggcc gaggatcggc acggcgccgc tcttcgcgag gtcgagcagc
      901 accgggtgct cctcgcggca gctcacgcac cacgaggccc agacgttcag cagccagacc
      961 ttgcccagca tgtcgcgctc ggagaaggtc ttgcccggct cgccgagctg cggcagatcg
     1021 aagtgcgggg ccggcttgcc gatgagaggc gagggcacct cgcgcgggtt gagcgtcagc
     1081 ccgatgccga gcagcgcgac caggaccgcg aagatgccga gcggaagaac gaagcggttc
     1141 atgagtggat tctcgtcggg atcaggccgt cgcggccgcg gcggcggcgc gctcttcgcg
     1201 ctccttgcgc gcggcgagcc ggtaacggcg gtcgctcgcc gcgagcacgc cgccgagcgc
     1261 catcagcagg cacccgcccc agatccagtc gacgaagggc ttcacctgcg cgcgcacgat
     1321 ccagccggtc gcgccgacct ggtcgccgag cgacacgtag agatcgcgcg tcggcccggg
     1381 ctggatcgcg gcctcggtca tcggcatgtt ctgcaccgtg tacaggcgct tttccgggta
     1441 cagcgtcgtc accggcttgc cgtcgcgctc gacgcggatc gacgcgcgcg cggcgatgta
     1501 gttggggccc tgcagctcgc gcacgccgtc gaagcggaac aggtagccgc ccaccgtcgc
     1561 cgtgtcgccc ggctccatgc gcacgtcctg ctcgctctcg tagcctttca ccagcgtgac
     1621 gcccacgacg aagaccgcga cgccgaagtg cgcaagcagc atgccgtaat gcgcgcgcgg
     1681 cgtgctcttc agccgtgtcc atgcggcctg cgcgcccgcc gcgtgggcgg tgcgctgcca
     1741 tagctgcagc acgctcgtga tcgcgatcca cgcggcgagc gcgagaccca gcgcgaccag
     1801 cgcgctccag cgcccgagcg cgaacggcag gagcagggcg gcgatcacgc tggcgccgaa
     1861 cgcccagcgc aggcgcttcc cgagctcggg aagctcggcc ttcttccagc gcgcgagcgg
     1921 cccgatgccc atgaggaaca gcgccggcgc catcagcggc acgaacacgg tctcgaaata
     1981 gggcgggccg accgagatct tgccgagccc catggcgtcg agcgccagcg gatacagcgt
     2041 gcccaggagg accgatcctg ccgcggcgac gagcatgacg ttgttcagca gcaggaaaga
     2101 ctcgcgcgag acggtgtcga agctcccgcc caggccgatc ttcggcgcgc gccagacata
     2161 gagcaggagc gagccgccga tcacgagaac cagcagcgcc aggatgaaga tgccgcgctt
     2221 ggggtcggtg gcgaaggcgt gcaccgacgt cagcaccccg gagcgcacca agaacgttcc
     2281 gagcaggctg agcgcgaacg cgaccagcgc aagcagcacg gtccagcttc ggaacgaccc
     2341 gcgcttctcg gtcaccgcga gcgaatggac gagcgcggtg ccgacgagcc agggcatgaa
     2401 cgaggcgttc tcgaccggat cccagaacca ccagccgccc catccgagct cggagtaggc
     2461 ccagccgctg ccgagcgcga tgccgaccgt gaggaaggac caggccacgg tcgtccacgg
     2521 gcgcgaccag cgcgcccagg cggcgtcgag ccggccgccc agcagcgcgg cgaccgcgaa
     2581 cgagaaggcg accgagaacc cgacgtatcc catgtagagc atcggcgggt ggatcaccat
     2641 ccccgggtcc tgcagcagcg ggttgaggtc gcggccgtcg gcgggcgcgg ggaacaggcg
     2701 caggaacggg ttcgacgtga acaacatgaa cagcaggaag ccgacgctca ccaggcccat
     2761 cacgccgagc acgcgcgcga tcatctcgtc cgggagccgg cggctgaacg cggcgaccgc
     2821 cacgctccac acggtgagca tcagcgtcca gagcaggatc gagccctcgt ggccgcccca
     2881 gacggcggtg atgcggtagt gcagcggcag cgacgagttc gagttcgatg cgacgtagag
     2941 cacgctgaag tcgttggcga cgaatgccca ggcgaggcag acgaaggcgg tcgcgacgaa
     3001 cacgaactgg ccgacggcga ccggccgcac cgcccgcatc cacgtcacgt tgccgcgcgc
     3061 ggcgccggcg agaccgagcg ccgactggca cagggcgagg cagagcgcga gcgccagcgc
     3121 gagctggccg atctcgggat tcatttggag cctccggcgg ggacgaccag cgtcttctcg
     3181 gcctgcgcct gggccttcgc gacggcgtcg gcggcggcgg ggggcatgta gttctcgtcg
     3241 tgcttggcga gcacctcgct cgcgacgaag gtgccgtcgg gaccgagcct gccctgcgtg
     3301 accacgccct tgccctcttt gaacaggtcc gggaggatgc cgcggtagac gaccttgacg
     3361 ctcttcgccg tgtcggtcac gacgaactgc acgctcacgc cgtcgggcag gcgcttcagg
     3421 ctgccggcct cgaccaggcc gccgatgcgg aacgcgcgac cgtgcggcac ctcgttcgcg
     3481 gcgatctgcg tcggggtgaa gaagaacacc aggttctcgc ggaaggcgct caggacgaag
     3541 gcggcggcga ccgccagggc cgcgaggccg ccggcgatca tggccaagcg cttgtgccgc
     3601 ggcttcatcg catcctcggc gactggcgct cggcgagcga gcggcggcgg cgcgcgagca
     3661 ggacgatctc ggcgaccagc agcgccagcg tgacgccgta ggagccccac acgtacaacc
     3721 cgtatccgcc catcgccagg aaattgtcga ggctgcccca gttcacgcgc gttctcccag
     3781 gtactccgtt gcccattcgg tgctgcgctc gcgctcgagc atgatgcagc gcgcgcgcgt
     3841 catcgccgcg gcgatgctgt agaaccagaa gccgagcgcc atcgcgagca tgccggcgag
     3901 catcgtcgcc gccatgctcg gcgccttcgt taggctgacc gaggcgccct ggtgcagcgt
     3961 gttccaccac ttcaccgaga agtagatgat cgggacgttg acgacgccga ccagcgcgag
     4021 caccgccccc gccttgtcgg cgcgccgcac gtcgtcgatc gacgcctgga gcgccatgaa
     4081 tccgaagtag aggaacagca ggatcagctc ggaggtgagc cgtgcgtccc agacccacca
     4141 cgtgccccag gtcgggcggc cccagagcga tcccgtccag agcgcgatga acgtgaagag
     4201 ggccccggtc ggcgcgatgg cccgggccat catggcggag agccgggtgt tgagcgcgag
     4261 accgaggccc gcccagaacg ccatcacgac ataaaggaac atcgacatcc atgccgccgc
     4321 gacgtggatg aagatgatgc ggtaggcctc gccctgctgt gcgtcggtcg gcgcgacgaa
     4381 gaagccgatg tagagcccga tcgcgcacgc gaccacggcg agcgcgccga acgccttcgc
     4441 cagcgcgccc gccagcggaa agaaggtctg cggagaggag tacttgtacc agttcactgc
     4501 cgtcgcggct cctgccctat tggatggcgc cagccacccg tttattccac cgaaatccgg
     4561 agcgccacgg ccgtcgccca gggagcgaag gcgcccgccg ccacgaggaa ggccgcgagg
     4621 agcagcagct gcgcttcccc gcccagcccg gaccccgctg cttccgccgc gccggcgccg
     4681 atgatcagca ccggcacgta cagcggcagc accaggagtg acaacagcgc tcctccgccc
     4741 cgcagcccca gcgtcaaggc cgcgccgatc gcgccgatca ggctcaggac cggcgttccg
     4801 agcgccaacg atacgaccag ggtgcccagc gatttttgat ccaggtcaaa ttgtagcgcc
     4861 aaaagggggg cgatcaggac caaaggcagg cccgaggcga gccagtgcgc ggccaccttg
     4921 gcggcgaccg tgacgccgag cggggtcgca ccgagcagca tctgctcgag cgtgccgtcg
     4981 gcgtggtcgg ccgcgaacac gcggtgcagg gcgagcatgg aggcgagcag cgccgcgacc
     5041 cagatgaccc cgggcgcgat catccgcagc agcgcgggat ccgggccgac gccgagcgga
     5101 aagaggctcg ccacgatgat gaagaagagg acgaccgtga acacgtccga gcggcggcgc
     5161 gcggcgagca gcaggtcgcg ccggaggatg caccgcacgg cggcgagcat cgtcagggcc
     5221 tcccgccgcc gaggtcgatg cgcgaggcgc gccccgcggc gatcgggacc tcgtggtgcg
     5281 tggtgtagag gaccgatccg ccgcgcgcga cgtgctcggc gatcagcgca cgcacgcgat
     5341 cggtcgcggc cgggtcgagc gcggtgaacg gctcgtcgag catccacaac gggatcgctc
     5401 gcgcgagcac cagccgcgcg agcgtcacgc gccgccgctg gccctgggaa agcaccctcg
     5461 ccggcagctc ctcgcagccc gcgagcccga gcgacgcgag cgcgccgcgc acgtccacgc
     5521 gcgcggccgc gatgccgccg agcgcgcagg cgatctcgag gttctcgcgg gcggtgagat
     5581 cgtccttcac cgcgggggcg tggccgaggt agacgagctc cctcgagtag tcctcgcgca
     5641 gccgccggac atcctcgccg cgccagcgga ccgcgccgcc gttcgcctcg agcagcccgc
     5701 aggcgatgcg gagcaggctc gtcttgccgc tgccgttcgc gcccgcgatg cgcaggaggt
     5761 cgccgccgcg cagcgtgaag gaaaggtcgg agaagagcgt gcgctcgccg cgcgtgcacc
     5821 ggagcgccgt ggcctcgagc gcggcgccgt cggtgagcag gctcacgcgg ctctaggctg
     5881 ccgcgagctc ggcgcgcact tcggcgagga gcctctcggc ctcgctgccg cggatgcgcc
     5941 cgatgtcgtc ctggtacttc agcagggtgc cgagcgtgtc gttcaccgtc tgcgcgtcga
     6001 gcgccagctt gtcgagggcg accagcgcct tcgaccagtc gatcgtctcg gcgacgccgg
     6061 gcagcttgaa caagtccatc ttgcgcaggc gctggacgaa ggcgacgacc tcgcgcgaga
     6121 gcttctcggc ggcctcgggc gccttgcgct tcaggatctc gatctcgcgc gccgcgtcgg
     6181 gatagtcgac ccagtggtag aagcagcggc gcttgatggc atcgtggatc tcgcgcgtgc
     6241 ggttcgaggt catcaccacg ataggcggct cggccgcttt gatcgtgccg atctccggga
     6301 tcgtcacctg gaagtcggac agcacctcga gcaggtaggc ctcgaagggc tcgtccgtgc
     6361 ggtcgagctc gtcgatcagc agcaccggcg cggcgcccgt gtcgccctcg agcgcctgca
     6421 agagcggccg gcggatcagg aacttctcgg agaagatgtc ctgcgagatg ccctcgcggc
     6481 tcttcacgcc ctccgcctcg gcgaggcgga tctcgatcat ctggcgcgag tagttccact
     6541 cgtagacggc cgaggcgacg tcgaggccct cgtagcactg cagccgcacg aggcgccggc
     6601 cgagcgtcgc ggcgaggacc ttcgcgatct cggtcttgcc gacgcccgcc tcgccctcca
     6661 gaaacagggg acgtcgcatc ttcagcgaca ggaacaccgc ggtcgcgagg ctgcgctcgg
     6721 caacgtaatc ggccgaggcg agcagcgact gggtgtcgtc gatggattgc ggcagggcgg
     6781 gcacggctcg attatatagc ccgggcaata aaaaaccctc cccagggaga ggggagggtc
     6841 ggcgggggtg cccctcgagt gatactcgag ccggcgggcc cgggagattc tgcccctgcc
     6901 atgccgccgc tctcggagca gcggcatccc cgggaaccgg ggactcccgg aaagcttcct
     6961 ctactttagc gcagactcga ccgcccggcg cgccatcacg gtaacgaggt gcgcgcggta
     7021 gtcggccttg gcgtgcaggt ccgagttcag gccgtcgggc ttcactttga cgttcgcgac
     7081 cgactcgggg gcgaacttcg acgtgagcgc cttctccatc tcgggcacgc ggaacacgct
     7141 cggcgccgcg cccgtgaccg cgacgcgcgc gcctttgccg gtatcggcga cgaggacgcc
     7201 cacgagcgcg aagcgcgacg ccgggttctt gaacttcatg tacgcggcgc gcttcgggat
     7261 cgggaacacc acggacgtga tcagctcgcc ctgttcgagc gccgtctcgt acataccctt
     7321 gaagaagtcg tcggcggcga tcttgcgctt gttggtcacg accgtggcgc ccaggcccag
     7381 cacgcccgcg ggatagtcgg cggcagggtc gttgttggcg agcgagccgc cgatcgtgcc
     7441 catgttgcgc acctggcggt cgccgatgct ggccgcgagc gaggcgagcg ccgggatggc
     7501 cttcctcacc tcggccgagc cggccacctc ggcgtggcgc gtcatcgcgc cgatcgtgac
     7561 gctgctgccg tcgaccttga tgcccttcag gtcgggcaga gtcccgagat cgatgacgtc
     7621 ggacggccgt gccaggcgca gcttcatcgc cgcgatcagg ctctggcctc cggcgagcgg
     7681 cttggcgtcg ccgttcttgg cgagcacggc ggcggcgtca gcgaccgatt tggccttctg
     7741 atagttgaac gtgtacatgc gtttctccta ggcagctttc cggttctgtt gcagcgtgcg
     7801 ccagatgcgc tgcggcgtcg ccggcatctc gatgtccttg accttgagcg cgtcggtgat
     7861 cgcgttcatc accgcggcgg gcgcgccgat cgcgcccgcc tcgccgcagc ccttcacccc
     7921 gagcgggttg tgcgtgcacg gggtctgctc ggtgccgacc ttgaacgacg gcacgtccga
     7981 cgcgcgcggc aggcagtagt cgagatagct cgcggtgacc agctgcccgc tctccgggtc
     8041 gtagtagcag ttctcggtga gcgcctgccc gatgccctgc gcgaggccgc cgtggacctg
     8101 gccttcgacg atcatcgggt tgatcacctt gccgaaatcg tcgaccgccg tgaaagcgac
     8161 gatctgcgag gcgccggtct cgcgatcgac ctccacttcg cagacgtagc tgcccgccgg
     8221 gaaggtgaag ttggtcgggt cgtagaacgc gttctcgttc agccccggct cgagcttgtc
     8281 gagcggatag ttgtgcggca cataggcggc aaaggcgacc tcgccgaaca tcttcttctt
     8341 gtcggtgccc gcgaccttga actcgccgcg gtcgtactcg atgtcggcct ccgacgcttc
     8401 cagcaggtgc gccgcaatct tcttgccctt ggcaatgatc ttgtcgagtg ccttgacgat
     8461 ggcggtgccg ccgactgcga tcgagcgcga gccgtaggtg cccatgccga agaggatctt
     8521 cgacgtatcg ccgtgcacga tctcgacctg ctcgatcccg aggccgagtc catccgccac
     8581 gacctgcgcg aacgtcgtct cg
//
LOCUS       DATDMW010000246         3481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1319286, whole genome shotgun sequence.
ACCESSION   DATDMW010000246 DATDMW010000000
VERSION     DATDMW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319286"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3327
                     /locus_tag="VI319_10600"
     CDS             <1..3327
                     /locus_tag="VI319_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY6864337.1"
                     /translation="KKYLEANHVKFHPGMNEDLAATAVWGTQQVNLFPDAKYDGVFGM
                     WYGKGPGVDRCGDVFKHANSAGTSKHGGVLVLAGDDHAAKSSTLAHQSEHIFKACCIP
                     VLNPASVQDYLDLGLHGFAMSRFSGCWIAFKCVTDVVESGAVVELDPARVQIVQPDFA
                     MPPGGLNIRWPDAFLDQEARLLDYKVYAALAYCRANRLDRIVWDSPRARLGIVTTGKS
                     FGDTMQALADLGIDERVARDAGIRVYKVAMSWPLEPHGARRFAEGLEEILVVEEKRQV
                     IEYQIKEELYNWREGARPPRVVGKFDDNGEWSIAAGQPAGNWLLPAHYELSPALIAKA
                     LANRFAKLGLDKALGEPYRQRIAYLEFKEKALTKPRIVENRLPYFCSGCPHNTSTKVP
                     EGSRAMAGIGCHFMAVWMDRNTSTFTHMGAEGAPWIGQAPFTECPHVFANLGDGTYYH
                     SGLLAIRAAVAAGVSMTYKILYNDAVAMTGGQHVDGPLDPAMISRQIAAEGVKPIVVV
                     YDEKEPLPEGLDWAPGVTFRERAEMDAVQRELREAKGVSAIIYVQTCATEKRRRRKRH
                     DFPDPPKRVIINDLVCEGCGDCSVQSNCLSVEPLETEFGRKRSINQSSCNKDYSCLNG
                     FCPSFVTVEGGSLKKGRAAAPGADAEAAFAALPDPAPLALERPHGTLVTGIGGTGVIT
                     IGQIMAVAAHIEGKGATVLDMSGLAQKFGPVMSHVRIARAPEELHSVRVGTGAADLVL
                     GCDLVVTSGGEALSKMDERSTKVLVNSTTSPTAAFVKNPDWRLPGAGLQDEISAAAGR
                     ANVDFVAAGRLATALMGDAIATNMFMLGYAYQKGWVPLGEPALLKAIELNGVSVDFNK
                     TAFAWGRRAAASPELVEKLASPSDVVPISQALSRDLGERVTRRAEFLAAYQDAAYARR
                     YTDLIERVRAAEARLGGDSLPLTEAVARYYFKLLAYKDEYEVARLHSDPAFRRRIETM
                     FEGDYRLKFHLAPPLLAKRDPATGELRKSVYGPWMMTAFRVLAKLKGLRGTALDVFGY
                     SEERRTERRLIAEYEGTVDELLARLDRGNRALAVEIASIPERIRGFGHVKARHLAEAK
                     KKEAELLSAFRDPRQQPKAA"
BASE COUNT          570 a         1227 c         1181 g          503 t
ORIGIN      
        1 aagaagtacc tcgaagcgaa ccacgtcaag ttccatcccg gcatgaacga agatctcgcc
       61 gctaccgccg tctggggcac gcagcaggtc aacctcttcc ccgacgccaa gtacgacggc
      121 gtcttcggca tgtggtacgg caaggggccc ggcgtcgacc gctgcggcga cgtcttcaag
      181 cacgccaact ccgccggcac ttcgaagcac ggcggcgtgc tggtgctcgc cggcgacgat
      241 cacgccgcga aatcgtcgac cctcgcgcac cagagcgagc acatcttcaa ggcgtgctgc
      301 atccccgtct tgaaccccgc gagcgtccag gactacctcg acctcgggct gcacggcttc
      361 gcgatgtcgc ggttctcggg ctgctggatc gctttcaagt gcgtcaccga cgtcgtcgag
      421 tcgggcgcgg tcgtcgaact cgatccggcc cgcgtgcaga tcgtgcagcc ggacttcgcc
      481 atgccgccgg gcggccttaa catccgctgg ccggacgcgt tcctcgatca ggaggcgcgg
      541 ctcctcgatt acaaggtgta tgccgcgctc gcctactgcc gcgcgaaccg cctcgaccgc
      601 atcgtctggg actccccccg ggcgcgcctc gggatcgtga ccaccggcaa gtcgttcggc
      661 gacacgatgc aggcgctcgc cgacctcggc atcgacgagc gcgtcgcgcg cgacgccgga
      721 atccgcgtct acaaggtcgc gatgtcgtgg ccgctcgagc ctcacggtgc gcgccgcttc
      781 gccgagggcc tcgaggagat cctggtcgtc gaggaaaagc gccaggtcat cgagtaccag
      841 atcaaggagg agctctacaa ctggcgcgaa ggcgcgcgcc cgccgcgcgt ggtcggcaag
      901 ttcgacgaca acggcgaatg gtcgatcgcc gccggccagc cggccggaaa ctggctgctg
      961 cccgcgcact acgagctctc tccggccttg atcgcaaagg cgctcgcgaa ccgcttcgcc
     1021 aagctcgggc tcgacaaggc gctgggcgag ccctatcgac agcgcatcgc ttatctcgag
     1081 ttcaaggaga aggcgctgac gaaaccgcgc atcgtcgaga accggctgcc gtacttctgc
     1141 tccgggtgcc cgcacaatac gtccaccaag gtgcccgagg gcagccgcgc catggccggc
     1201 atcggctgtc acttcatggc ggtctggatg gaccgcaaca cctcgacctt cacgcacatg
     1261 ggcgccgagg gtgcgccgtg gatcggccag gcgccgttca ccgagtgccc gcacgtgttc
     1321 gccaatctcg gggacggcac gtactatcat tcgggcctgc tcgcgatccg cgccgccgtc
     1381 gccgccggcg tcagcatgac ctacaagatc ctgtacaacg acgcggtcgc catgacgggc
     1441 ggccagcacg tcgacgggcc gctcgacccg gcgatgatct cgcgccagat cgcggccgag
     1501 ggcgtgaagc cgatcgtggt ggtctacgac gagaaggaac cgcttccgga agggctcgac
     1561 tgggcgcccg gcgtcacctt ccgcgagcgc gccgagatgg acgcagtgca gcgcgagctg
     1621 cgcgaggcga agggcgtgtc ggcgatcatc tacgtgcaga cctgcgccac cgagaagcgt
     1681 cggcgccgca agcggcacga ttttccggat ccgcccaagc gggtgatcat caacgacctg
     1741 gtgtgcgagg gctgcggcga ctgcagtgtg cagtcgaact gcctttcggt cgagccgctc
     1801 gagaccgagt tcggccgcaa gcgcagcatc aaccagtcct cgtgcaacaa ggactactcg
     1861 tgcctgaacg gtttctgccc cagcttcgtc accgtcgagg ggggaagcct gaagaaaggc
     1921 cgcgccgcgg cgcccggcgc cgacgccgag gccgccttcg cggcgctgcc ggacccggcg
     1981 ccgcttgcgc tcgagcggcc gcacggaacg ctcgtgacgg gcatcggcgg cacgggcgtc
     2041 atcacgatcg gccagatcat ggcggtggcc gcgcacatcg aaggcaaggg cgcgacggtg
     2101 ctcgacatgt cgggcctcgc gcagaaattc ggaccggtca tgtcgcacgt gcgcatcgcg
     2161 cgcgcgccgg aggagctgca ttcggtgcgc gtcggcaccg gcgccgcgga cctcgtgctg
     2221 ggatgcgacc tggtggtcac ctcgggcggc gaggcgctct cgaagatgga cgagcgctcg
     2281 accaaggtgc tcgtgaactc gacgacgtcg ccgacggcgg cgttcgtgaa gaacccggac
     2341 tggcgcctcc ccggcgcggg cctgcaggac gagatctcgg ccgcggccgg ccgcgccaac
     2401 gtcgacttcg tcgcggccgg gcgcctcgcg accgcgctga tgggggacgc gatcgcgacc
     2461 aacatgttca tgctcggcta cgcctaccag aaaggatggg tcccgctcgg cgagccggcg
     2521 ctgctgaagg cgatcgagct gaacggcgtc tcggtcgact tcaacaagac ggccttcgcg
     2581 tggggacgcc gcgccgcggc gagccctgag ctggtcgaga agctcgcctc cccgtccgac
     2641 gtggtcccca tctcgcaggc gctgtcgcgc gacctcggcg agcgggtgac gcgccgcgcc
     2701 gagtttctcg cggcctacca ggacgccgcc tacgcgcgcc gctataccga tctcatcgag
     2761 cgcgtgcgcg cggcggaggc gcggctcggc ggcgactcgc tgccgctgac cgaggcagtc
     2821 gcccgctact acttcaagct gctcgcctac aaagacgagt acgaggtcgc gcgcctgcat
     2881 tcggacccgg ccttccgccg gcgcatcgag acgatgttcg aaggcgacta caggctgaag
     2941 ttccacctcg cgccgcccct gctggcgaag cgcgatcccg cgaccggcga gctccgcaag
     3001 tccgtctacg gcccgtggat gatgacggcc ttccgggtcc tcgcgaagct gaaggggctg
     3061 cgcgggaccg cgctcgacgt cttcggctac tcggaagagc gccgcaccga gcgccggctc
     3121 atcgccgagt acgaaggcac ggtggacgag ctcctcgcgc ggctggaccg cggcaaccgc
     3181 gcgctcgcag tcgagatcgc ctcgatcccc gagcgcatcc gcggcttcgg ccacgtgaag
     3241 gcgcggcacc tcgccgaggc gaagaagaag gaggccgagc tgctgtccgc gttccgcgat
     3301 ccgcgccagc agccgaaagc cgcctgagcg ccgacgcatg acgctgacgc gagccgatct
     3361 cgagaacgac cgcttgcgga agctgttccg cgaggccaat cccgacacgc cggtgctgtc
     3421 cgaggcggag ctcgaggcct cggcgagcgc gctcctcgcc gagcatccgc aaggcgagga
     3481 c
//
LOCUS       DATDMW010000247         8149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2442510, whole genome shotgun sequence.
ACCESSION   DATDMW010000247 DATDMW010000000
VERSION     DATDMW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442510"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1482)
                     /gene="kaiC"
                     /locus_tag="VI319_10605"
     CDS             complement(7..1482)
                     /gene="kaiC"
                     /locus_tag="VI319_10605"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEY6864338.1"
                     /translation="MPAPAATTTLRKTPSGIRGLDEITGGGLPAGRPSLVCGGPGCGK
                     TLFGIEFLVRGALEYGEPAVLMTFEERAEDLIANTRALGFDLDDLVRRKLLAIDYVKI
                     ERAEIQETGEYDLEALFVRLNYAIESTGAKRVLLDTVESLFAGLSNEGVLRSELRRLF
                     LWLKEKGVTAVVTGERGAGTLTRYGLEEYISDCVILLDHRVTDQVSTRRLRVVKYRGS
                     MHGTNEYPFVIDRDGITVVPITSAGLAHSASSERVSSGVDGLDTMLCGGYYRGSTILI
                     TGTAGTGKSSLAARFAEAAGERGERGLYFAFEESRNQLVRNMGSLGIELERHIETGEL
                     TFSCSRPTLVGLEAHLSGMIRQIDALRPSVIVVDPVSSLLTGSEALSVQAMLIRLVDY
                     VKSQGITMVMTSLLDEGMAAGTETAVSSIVDTWIVLRDVETHRDRQRLLYVLKARGIG
                     HSRRIHEIHMSHKGIEVLGLQPSAAPRGRTRAARPRSRRAR"
     gene            complement(1609..2832)
                     /locus_tag="VI319_10610"
     CDS             complement(1609..2832)
                     /locus_tag="VI319_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864339.1"
                     /translation="MSAFPKTPLAAFPAIALLAGAAHAQANPDGKAIAAAKCNSCHAL
                     EARVGNGYTPKGWSTVLRMMSNQGAPISKADIAALHPYLAKTFPEKNKAAAAIVPGPA
                     KVSFKQWPAATPGSRPHDPLAARDGSLWYTGQMANVLGRVDPRTGKVREYKLKTAHSG
                     PHGLKEDAQGNIWYTGNTGALIGKLNPKTGEVTEYPMPDPEAKDPHTLLFAKDGILWF
                     TVQNANRIGRLDPKTGEIKLLTPPTPKSRPYGMAFDSKGTLFVVQFGANSIAAVDPAT
                     DQIKEYKLPAAGARPRRVAITSDDIVWYTDYSRGYLGRLDPKTGEVKEWPSPSGAKSA
                     PYGISSIDDVLWYSESEASPGTIVRFDPKTEKFQTWKIPGGGNIVRNTDVTSGHDWVL
                     ANSLNNQVTLVRIVK"
     gene            complement(2921..3460)
                     /locus_tag="VI319_10615"
     CDS             complement(2921..3460)
                     /locus_tag="VI319_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY6864340.1"
                     /translation="MPRLASAELPGVPLHLVQRARGRAPCFSGPADRRAYLEGLAAGA
                     AQEGCALHAYVLMGNHVHLLVTPARADAGGRLMRTLVERYARHLAQAYGHEGDVWDAR
                     YDGTPVHPRRYLLTCMGYIELNPVRAGMVASPGAYRWSSYRANAEGADDPLLTPHPAY
                     VALGRSRAERCAAYRATLE"
     gene            3565..4140
                     /locus_tag="VI319_10620"
     CDS             3565..4140
                     /locus_tag="VI319_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY6864341.1"
                     /translation="MATASTPSLPDRRNELTRRLILESALQVLERSSFGGLTMGSVAK
                     RARMSLRTVFRYFPTREVFLDAIAEQLRLLLGLPPPQTVDELVAFPRALYTGFEAHAR
                     LMRAALHSELYDRMRETAARARWEAVRRIVDSTAPGRSDEDRKVAAANIRFYLGASTW
                     HYYRAHFDFTLEEAIRAAETAIRQSLEGLRA"
     gene            4254..5240
                     /locus_tag="VI319_10625"
     CDS             4254..5240
                     /locus_tag="VI319_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEY6864342.1"
                     /translation="MARLAAAFGSSHSVMLAAGLDDWLTGFRESDPRMPYYDPEGRPS
                     SYADVLARAPSDAQERVRPEAIRARFEETQAAMARVKREIEDARLDALVIVGDDQHEL
                     FGDRHMPSIAVYHGETIRNAPRPAAPAADWYRRAQLRRLEDGPEAHYPCHQRLALHLI
                     EGLVEREFDVSAVAGLAPDEHEGHAYSFVHRWYLNGTPLPVVPIFLNTYNPPNPPLPR
                     RCARLGAALKDLIESYPEDIRVGMLASGGLSHFVVDEDLDRGVIDALKRKDMDWLGRL
                     DPRRLKAGSSEIRNWIVAAAAASDLELAWMAYIPSYRTAALTGIGLGFAKWS"
     gene            5311..5631
                     /locus_tag="VI319_10630"
     CDS             5311..5631
                     /locus_tag="VI319_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY6864343.1"
                     /translation="MGKKLYVGNLAYSVGDADLQKMFAAHGTVRSAQVITDRATGQSK
                     GFGFVEMGTDQEAQAAIAALNGKQMGGRTLTVNEARPKEGGGGGGGGGRGFGGGGGGG
                     GRRF"
     gene            5760..6812
                     /locus_tag="VI319_10635"
     CDS             5760..6812
                     /locus_tag="VI319_10635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY6864344.1"
                     /translation="MAKKNDPGERTSARPDPSAEETARLLALRSYCVLDTGREGRFDD
                     LTALASSICETPVSLVSLVDSNRLFFKSEHGLGVREVPDTGTGFCRYAIRQRDIFEIP
                     DTREDPRFVQHPLVTSAPHVRFYAGAPLITPDGIAVGTLCVIDFRPRKLDARQTEALR
                     ILARQVIAQLELNLQAMRDPLTGLYNRRPLEEALHRELLRARRSGSTVGLMAIDLDRF
                     KHVNDSLGHDAGDAVLRTIARELMACVREEDIVCRAGGEEFVVILPGTGEYALRERAE
                     LVRRTVEHEAITTFTNPLQVTVSIGLALYPGHGATTTALMRAADAALYQAKGAGRNCI
                     VMADLQGDQLTLWGTA"
     gene            6854..>8149
                     /locus_tag="VI319_10640"
     CDS             6854..>8149
                     /locus_tag="VI319_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6864345.1"
                     /translation="MVNPSGFVERKFKGRREDLRLITGQGRYSADWNAPGQLHGFFLR
                     SDRAHALIRSLSTEDALRSPGVVAVLTGRDAIEAGYGTAPTFLRAPGRGGMQLRTPHR
                     PVLAHERVRYAGQEVALVVAQTALQAQDAAERIEIEYEELPLVLDPERALEAGAPTIH
                     AEAPDNICYDYEYGDAGRTDAAFSRAARVTRVVLELQRMAGNPMEPKAALASWDAATD
                     TYHLYSPTQGLSLTTHALSTVMRVPRERLRVHAQDVGGGFGIRTDAYPEYIALLLASR
                     RLGRPVKWVSSRSETFLSDYHARAAKLIGELALDRDGTFLGMRIRWIVDNGAYNSMPG
                     PLINSINPSTHMVNAYRIPALHGRHTLVFTNTTPTTAYRGAGRPNVSYLVERLVEEAA
                     REAGIDRIELRRRNMIPKDAFPYTTPVGSVYDSGDPPGEL"
BASE COUNT         1188 a         2853 c         2851 g         1257 t
ORIGIN      
        1 gggtatctac cgcgcccgcc gcgaccgggg acgggccgcg cgcgtccgcc cgcggggggc
       61 ggcgctcggc tgcaggccga gcacctcgat ccccttgtgg ctcatgtgta tctcgtggat
      121 gcgccgcgaa tgcccgatgc cgcgcgcctt gagcacgtac agcaggcgct ggcggtcgcg
      181 gtgcgtctcg acgtcgcgca ggacgatcca cgtgtcgacg atcgaagaca ccgcggtctc
      241 cgtgcccgcc gccatgcctt cgtcgagcag gctcgtcatc accatggtga tgccctggct
      301 cttcacgtag tcgaccaggc ggatcagcat ggcctgcacc gacagcgcct cgcttccagt
      361 cagcaggctg ctcaccggat cgacgacgat gaccgacggc ctgagcgcgt cgatctgccg
      421 gatcataccc gagagatgcg cctccaggcc gacgagcgtc gggcggctgc acgagaacgt
      481 gagctcgccg gtctcgatgt gccgctcgag ctcgatcccg agcgagccca tgttgcgcac
      541 gagctgattc cgcgattcct cgaacgcgaa atacaggccg cgctcgccgc gctcgcccgc
      601 ggcctcggcg aagcgcgccg ccaggctgct cttgcccgtg cccgcggtgc ccgtgatgag
      661 gatggtgctg ccgcggtagt agccgccgca tagcatcgtg tccaggccgt cgaccccgct
      721 cgagacacgt tcgctgctcg ccgagtgggc gagcccggcc gaggtgatcg ggacgacggt
      781 gatgccgtcg cggtcgatca cgaagggata ctcgttggtg ccgtgcatcg agccgcggta
      841 tttgacgacg cgcagccgcc gcgtcgacac ctggtcggtg acgcggtggt cgagcaggat
      901 cacgcagtcc gagatgtact cctccaggcc gtagcgcgtg agcgtgccgg cgccgcgctc
      961 ccccgtgacc acggcggtca cgcccttctc cttcagccac aggaacaggc gccgcagctc
     1021 cgagcgcagc acgccctcgt tcgacagtcc cgcgaacagc gactcgacgg tgtcgagcag
     1081 gacgcgcttc gcgccggtcg actcgatcgc atagttcagg cgcacgaaca gggcctcgag
     1141 gtcatactcg cccgtctcct ggatctcggc gcgctcgatc ttcacgtagt cgatcgcgag
     1201 cagcttgcgc cgcaccaggt cgtcgaggtc gaagccgagg gcgcgcgtat tcgcgatcag
     1261 gtcctcggcg cgctcctcga aggtcatcag cacggcgggc tcgccgtatt ccagggcccc
     1321 gcgcaccagg aactcgatgc cgaatagcgt cttgccgcag cccggaccgc cgcagaccag
     1381 cgacggacgc ccggccggca ggccgccgcc ggtgatctcg tccaggccgc ggattccgga
     1441 gggcgtcttt cgcaacgtgg tcgtcgccgc cggggcgggc atctgggtga gctccttggt
     1501 tcgaaggatc gaggcgccgc gcgggcttgt gcgcgacgct gcgaatatac cgcaagccca
     1561 tgaaaaacgg cggagatcgg tctccgccgt tctcgcccgc ggagcggatt acttcacgat
     1621 ccggaccagc gtcacctggt tgttcaggct gttcgcgagc acccagtcgt gaccgctcgt
     1681 gacgtcggtg ttccgcacga tgttcccgcc gcccgggatc ttccaggtct ggaatttttc
     1741 ggtcttcggg tcgaagcgca cgatcgtgcc cgggctcgcc tccgactcgc tgtaccagag
     1801 cacgtcgtcg atcgacgaga tgccgtaagg cgccgacttc gctccgctcg gcgagggcca
     1861 ttccttcacc tcgcccgtct tcggatcgag acggccgagg tagccgcggg aataatccgt
     1921 gtaccacacg atgtcgtcgc tggtgatggc aacgcgccgc gggcgcgcgc cggcggcggg
     1981 cagcttgtac tccttgatct ggtcggtcgc gggatcgacg gccgcgatgc tgttcgcgcc
     2041 gaactgcacg acgaagagcg tccccttgga gtcgaacgcc atgccgtacg ggcgcgactt
     2101 gggcgtcggc ggcgtgagca gcttgatctc gcccgtcttc ggatcgagac gcccgatccg
     2161 gttcgcgttc tgcacggtga accagaggat gccgtccttc gcgaagagca gggtgtgcgg
     2221 gtccttcgct tccgggtcgg gcatcgggta ctcggtcact tcgcccgtct tcgggttcag
     2281 cttgccgatc agcgcgccgg tgttgccggt gtaccagatg ttgccctgcg cgtcctcctt
     2341 cagcccgtgc ggcccggagt gcgcggtctt cagcttgtac tcgcgcacct tgccggtcct
     2401 gggatcgact cggccgagga cgttggccat ctggcccgtg taccacagcg agccgtcgcg
     2461 cgcggcgagc gggtcgtgcg gccgcgagcc cggcgtggcc gcgggccatt gcttgaacga
     2521 gaccttggcc gggccgggga cgatcgcggc ggccgccttg ttcttctcgg ggaatgtttt
     2581 cgcgagataa ggatgcagcg cggcgatgtc cgccttgctg atcggtgcgc cctggttgct
     2641 catcatgcgc agcacggtgc tccagccctt gggcgtatag ccgttgccga cgcgcgcctc
     2701 gagcgcgtgg cagctgttgc atttcgccgc cgcaatcgct ttgccgtccg ggttcgcttg
     2761 cgcgtgcgcc gcgccggcga gcaatgcgat cgccgggaac gccgccagcg gcgtcttcgg
     2821 aaatgcgctc atggatttcc tcctgtcgtt gcggagcggg gtagggcggc cattcttgca
     2881 cagcgcgcgc gggcgcacga tgttgaccgg agaggcaatg tcactccagc gttgcgcggt
     2941 acgcggcgca gcgctccgcg cgcgagcgcc cgagcgcgac gtacgcggga tgcggcgtga
     3001 gcagaggatc gtcggcgcct tcggcgttcg cccggtagct cgaccaccga taggcacccg
     3061 ggctcgccac catgcccgcg cggacaggat tgagctcgat gtagcccatg caggtgagca
     3121 gatagcgcct cggatgcacc ggcgtgccgt catagcgcgc gtcccagaca tcgccctcgt
     3181 ggccgtaggc ctgggccagg tgacgggcgt agcgctccac gagggtgcgc atcagccgcc
     3241 ccccggcgtc cgcgcgagcc ggcgtgacga gcaagtgaac gtggttgccc atgaggacgt
     3301 acgcgtgcag ggcgcagccc tcctgggccg cgcctgccgc caggccctcg aggtaggccc
     3361 ggcgatcggc gggtccggaa aagcaggggg cccggccccg ggcccgctgg acgaggtgca
     3421 gcgggacgcc ggggagttct gcggaggcga ggcgcggcat ggcccgacca acgccgccgc
     3481 gcccacccgc gttgacgcgg cgctcgttga aactcagaag gcaatgtcat acgatatgac
     3541 atcgtgtcat aggatatgcc agacatggcg accgcgtcga cgccttcgct tcccgaccgc
     3601 cgcaacgagc tcacgcgccg gctcattctc gagtcggcgc tgcaggttct cgagcgcagc
     3661 tcgttcggcg gcctgaccat ggggtcggtg gcgaagcgcg cgcgcatgtc gctgcgcacg
     3721 gtgttccgct acttcccgac gcgcgaggtc ttcctcgacg cgatcgccga gcagctgcgt
     3781 ctgctgctcg ggctgccgcc cccgcagacg gtggacgagc tggtcgcctt cccccgggcg
     3841 ctctataccg ggttcgaagc gcacgcgcgc cttatgcggg ccgcgctcca ctccgagctg
     3901 tacgaccgca tgcgcgagac ggccgcgcgg gcgcgctggg aagccgtgcg gcgcatcgtc
     3961 gactcgaccg cgcccggccg cagcgacgag gaccgcaagg tcgccgccgc gaacatccgg
     4021 ttctacctgg gcgccagcac ctggcactac taccgggccc acttcgactt caccctggaa
     4081 gaggcgatcc gggcggccga gaccgccatc cgccagtcgc tggagggcct gcgcgcctga
     4141 ccctcgccgc cttttcggcg aggtgacaaa ccggcccggg gttgcggtac acttgcccgc
     4201 aatccgcccc ggcctcctgc ctcttccttc cccccgcccg ctcccgttct gtcatggccc
     4261 gcctagccgc cgcgttcggc tcgtcgcaca gcgtcatgct cgctgcggga ctcgacgatt
     4321 ggctcacggg cttccgggag agcgatccgc gcatgccgta ctacgacccg gaagggcgcc
     4381 cgagcagcta cgccgacgtg ctcgcccgcg ccccgtcgga tgcgcaggag cgcgtgaggc
     4441 cggaggcgat ccgcgcccgc ttcgaggaga cccaggccgc gatggcacgg gtgaagcgcg
     4501 agatcgagga cgcgcggctc gacgccctgg tcatcgtcgg cgacgaccag cacgagctct
     4561 tcggcgaccg gcacatgccg tcgatcgccg tgtaccacgg cgagaccatc cggaacgcgc
     4621 cgcgcccggc cgcgccggct gccgactggt atcgccgggc gcagctgcgg cgtctggagg
     4681 acggccccga ggcgcactat ccctgccacc agcgactcgc gctgcatctg atcgagggcc
     4741 tggtcgagcg cgagttcgac gtctcggcgg tcgccggact cgccccggac gagcacgagg
     4801 ggcacgccta ctcgttcgtg caccgctggt atctgaacgg gacgccgctg ccggtcgtgc
     4861 cgatcttcct caacacgtac aacccgccga acccgccgct cccgcgccgc tgcgcccggc
     4921 tcggcgccgc gctcaaggac ctgatcgaga gctatcccga ggacatccgg gtcgggatgc
     4981 tggcctccgg cggtctgagc catttcgtcg tcgacgagga cctggaccgc ggcgtgatcg
     5041 acgcgctcaa gcgcaaggac atggactggc tcgggcggct cgacccgcgg cgcctgaagg
     5101 cgggcagctc ggagatccgc aactggatcg tcgcggcggc ggcggcgagc gatctcgagc
     5161 tggcgtggat ggcctacatc ccgtcgtacc gcaccgcggc gctgacgggg attggactcg
     5221 gtttcgcgaa atggagttga tttttgccgg ctgaaggcgc cgcccgtggg gggcggcgcg
     5281 gaatgacggt gttcacagga gcagatcgat atgggcaaga aactctacgt cggcaacctg
     5341 gcgtactcgg tgggtgacgc ggacctgcag aagatgttcg cggcgcacgg cacggtgcgc
     5401 tccgcgcagg tcatcaccga tcgcgcgacc gggcagtcga aaggcttcgg cttcgtcgag
     5461 atgggcacgg accaggaggc gcaggcggcg atcgccgcgc tgaacggcaa gcagatgggc
     5521 ggccgcacgc tgaccgtgaa cgaggcgcgg cccaaggagg gcggcggcgg gggcggcggc
     5581 ggcggccggg gcttcggcgg tggtggtggc ggcggcggac gccgattctg acggctcgct
     5641 ttcgtttttc gagccgcgac aaggccctgt gcctcgcagg gtcgaggggt ggtaactctt
     5701 cctttgccgc ggttgatcgg tcggtgtaga tttcggcttc cccaagggag ggggccggta
     5761 tggccaaaaa gaacgacccg ggcgagcgca cctcagcccg gccggacccc agcgccgagg
     5821 aaaccgcacg attgctggcg ctgcggagct actgcgtgct cgataccggc cgcgagggcc
     5881 ggttcgacga cctgaccgcg ctcgcctcga gcatctgcga gacgccggtg tcgctcgtca
     5941 gcctggtcga ctccaaccgc ctgttcttca agtccgagca cgggctcggc gtgcgcgagg
     6001 tccccgacac cggcaccggc ttctgccgct acgcgatccg ccagcgcgac atcttcgaga
     6061 tccccgatac gcgcgaagac ccccgcttcg tccagcatcc gctggtcacg agcgcgccgc
     6121 acgtgcgctt ctacgcgggc gccccgctca tcacgccgga cggcatcgcc gtcggcacgc
     6181 tctgcgtgat cgacttccgg ccgcgcaagc tcgacgcgcg gcagaccgag gcgttgcgca
     6241 ttctggcgcg ccaggtgatc gcgcagctcg agctgaacct gcaggcgatg cgcgatccgc
     6301 tcaccgggct gtacaaccgc cgtccgctcg aggaggcgct gcaccgcgag ctgctgcgcg
     6361 cgcgccgcag cgggtcgacc gtcgggctga tggcgatcga cctcgaccgc ttcaagcacg
     6421 tcaacgacag cctcggccac gacgccggcg acgcggtgct gcgcacgatc gcgcgcgagc
     6481 tgatggcgtg cgtgcgcgag gaggacattg tgtgccgcgc gggcggcgag gagttcgtcg
     6541 tgatcctccc gggcaccggg gaatacgctc tccgcgagcg cgcggagctc gtgcgtcgga
     6601 ccgtcgagca cgaggcgatc acgaccttca ccaacccgct gcaggtcacg gtgtcgatcg
     6661 ggctggcgct gtatccgggc cacggcgcga ccacgacggc gctgatgcgc gcggccgacg
     6721 cggcgctcta ccaggcgaag ggcgccgggc ggaactgcat cgtcatggcc gacctccagg
     6781 gcgatcagct caccctctgg ggcacggcct gagcgtgcgc ggccggggct cgcccggctg
     6841 ctagaatccg ccggtggtga atccgagcgg cttcgtggaa agaaagttca agggacggcg
     6901 cgaagacctg cgcctgatca cggggcaggg acgctattcc gccgactgga acgctccggg
     6961 acagctccac ggcttcttcc tgagatcgga ccgcgcgcac gcgctcatcc gctcgctgag
     7021 caccgaggac gccttgcgct cgccgggcgt cgtcgccgtg ctcaccgggc gcgacgcgat
     7081 cgaggcggga tacgggacgg cgccgacctt cctgcgcgcg cccggccgcg gcggaatgca
     7141 gctgcggacg ccgcaccggc cggtgctcgc gcacgagcgc gtgcgctacg ccggccagga
     7201 agtcgcgctg gtcgtggcgc agaccgcgct gcaggcgcag gacgccgccg agcgcatcga
     7261 gatcgagtac gaggagctgc cgctcgtgct cgaccccgag cgcgcgctcg aggccggggc
     7321 gccgacgatc catgccgagg cgcccgacaa tatttgctac gactacgaat acggcgacgc
     7381 cggcaggacc gacgcggcgt tctcgcgcgc cgcgcgcgtc actcgggtgg tcctcgagct
     7441 gcagcgcatg gcgggcaatc ccatggagcc caaggccgcc ctcgcgtcct gggacgcggc
     7501 gaccgacacc tatcacctgt actccccgac gcagggtctc tcgctcacga cgcacgcgct
     7561 cagcaccgtg atgcgcgtgc cgcgcgagcg gctgcgcgtg cacgcgcagg acgtgggcgg
     7621 cgggttcggc atccgcaccg acgcgtatcc ggagtacatc gcgctgctgc tcgcgtcgcg
     7681 caggctgggc cgcccggtga agtgggtgtc gtcgcgctcc gagaccttcc tgtccgacta
     7741 ccacgcgcgc gcggcgaagc tgatcggcga gctcgcgctc gaccgcgacg ggaccttcct
     7801 cggcatgcgc atccgctgga tcgtcgacaa cggcgcctac aactcgatgc ccgggccgct
     7861 gatcaactcg atcaatccgt cgacgcacat ggtgaacgcg taccggatcc cggcgctgca
     7921 cggccgccac acgctcgtat tcacgaacac gacgccgacg acggcgtacc gcggggccgg
     7981 gcgcccgaac gtcagctacc tggtcgagcg cctggtcgag gaggcggcgc gcgaggcggg
     8041 catcgaccgc atcgagctgc gccggcgcaa catgatcccg aaggacgcgt tcccgtacac
     8101 gacgccggtg ggctcggtgt atgacagcgg ggatccaccc ggcgagctc
//
LOCUS       DATDMW010000248        25955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2317139, whole genome shotgun sequence.
ACCESSION   DATDMW010000248 DATDMW010000000
VERSION     DATDMW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25955
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317139"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /gene="eno"
                     /locus_tag="VI319_10645"
     CDS             <1..862
                     /gene="eno"
                     /locus_tag="VI319_10645"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY6864346.1"
                     /translation="MPVPMMNVINGGAHANNSLDIQEFMIVPVGASTFRDALRCGAEI
                     FQALKKLLDAKGLSTTVGDEGGFAPSLPNNEAAIKLALEAISKAGYQPGTDVLLALDC
                     ASSEFFKDGKYVLESENQSLAPGKFADVLASWADKYPIVSIEDGMAESDWDGWRGLTE
                     RLGAKVQLVGDDLFVTNTKILREGIQRGIANSILIKVNQIGTLTETFAAIEMAKRAGY
                     TAVISHRSGETEDTTIADIAVGMNTLQIKTGSLSRSDRIAKYNQLLRIEEDLGDAVGY
                     PGRGAFYQLR"
     gene            859..1149
                     /gene="ftsB"
                     /locus_tag="VI319_10650"
     CDS             859..1149
                     /gene="ftsB"
                     /locus_tag="VI319_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEY6864347.1"
                     /translation="MRPLAAVLAALVLLLQYPLWLGKGGWLRVWDLDRQVQAQKDQNA
                     KLRLRNEALDAEVRDLKQGLDALEERARYELGMVGYEETFFQLVPRPSDARK"
     gene            complement(1171..2160)
                     /locus_tag="VI319_10655"
     CDS             complement(1171..2160)
                     /locus_tag="VI319_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY6864348.1"
                     /translation="MTLGENAHARRLRHFAFNGDADGLCAVQQLRLAQPGPANLVTGV
                     KRDITLLRHLAPPAGDRVFVLDVSLHENRGELMRLLDSGIRVTYFDHHRAGAIPAHPG
                     LDAHIDESADVCTSILVDRHLEGRYRRWAVVAAFGDNLTTVARAMAEEAGLTEAQATD
                     LERLGVCLNYNAYGESVEDLHFNPAELSARLLPYTDPFAFMTDAPVYEKLRAGYEDDM
                     AKARGAAARVYGPSGIVVLPNAPWARRAIGVLINELARERPNVALAILSPKSAGGYTV
                     SVRVPPTSAVSAADFCKDFPTGGGRKSAGGINHLPDGDVEGFAARFEARFRTR"
     gene            complement(2446..3027)
                     /locus_tag="VI319_10660"
                     /pseudo
     CDS             complement(2446..3027)
                     /locus_tag="VI319_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            2990..3124
                     /locus_tag="VI319_10665"
     CDS             2990..3124
                     /locus_tag="VI319_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864349.1"
                     /translation="MSDENPYPSAPFTKAAKNVIAIRAMEAVSRGGMIESSPVIAAQK
                     "
     gene            complement(3115..4023)
                     /gene="cysD"
                     /locus_tag="VI319_10670"
     CDS             complement(3115..4023)
                     /gene="cysD"
                     /locus_tag="VI319_10670"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004694369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY6864350.1"
                     /translation="MSEKSDDYLDELEAEAIYILREAIAQAKWPVLLFSIGKDSCALL
                     HLARKAFFPAPLPFPVLHIDTKWKFREMIAFRDTEARRLGFNLLVHTNPDGLAQGINP
                     IDHGSRVHTEIMKTLALRQALEAGCYDCVIGGARRDEEASRAKERIFSYRDTHHQWDP
                     RHQRPELWRLYNARVGAGESMRVFPLSNWTELDVWKYVRRERIPLVGLYYAKRRPTVI
                     RDGMIIMVDDERLPLAPGEAPEDRMIRFRTLGCYPLSAAVVSHATTLDQIIAELEASP
                     DSERQGRLIDKDQVGAMERKKREGYF"
     gene            complement(4016..5959)
                     /gene="cysC"
                     /locus_tag="VI319_10675"
     CDS             complement(4016..5959)
                     /gene="cysC"
                     /locus_tag="VI319_10675"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY6864351.1"
                     /translation="MAYGDQQVVRKNGRDGVSEAHTRRGRQHAGRSKDISNARIFRFL
                     TCGSVDDGKSSLIGRLLKDLSLVPEDTWESVLRESERRNFSRSNPDYSLLLDGLLIER
                     EQGITVDIAWRYFATPNRKFIVADCPGHEHYTRNMVTGASHCDGALVLIDARKGVLAQ
                     TRRHLSVVAMMRVGSVLIAVNKMDLVDWDRVRFDAIRREALEYAHKLGLSDVTVIPAS
                     ATHGGNVVRKAAETPWYAGLTISEALEAMPNRRPPSCSARLVAQWVNRPNQDFRGIAG
                     EVLGAPLRVGDTVRALPSGIYARIDRLCTFAGDLQIARPGQPVTVVLDKELDVARGDW
                     LVKATDVPPAVTDHIEANVVWMSDTPLVPGRRYDFQAGCTIVPGSVRRIIHKLDVETL
                     GSQEASRLACNDVGTCEIVLERSVPVDAYADSVQTGSLIFIDRTTFRTEGAAMVSTVA
                     AKNISWHETAVTRSARASIKGHSPQVIWLTGLSGAGKSTIANALEARLNAAGVHTMLL
                     DGDNVRHGLNRDLGFSEADRTENIRRVGETAKLLLDAGLVVITAFISPFRRERDLVRE
                     LVADGEFLEIFVDTPLAVCEQRDPKGLYQRARRGQLPEFTGISQAYEPPSSPDVRIDT
                     STSTVAQSVESILVSLKARGIHV"
     gene            complement(6023..7042)
                     /locus_tag="VI319_10680"
     CDS             complement(6023..7042)
                     /locus_tag="VI319_10680"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY6864352.1"
                     /translation="MAIFMIGIQRSGSNLLRLMLNELPEIAAPHPPHILQRMMPLVPY
                     YGDLGKGQSFSLLVDDVCRLVELNPVPWEGVVLDRKDVAARCRKHSLVAVFGAVYDIM
                     AEAWKAKTWCCKSLANVYYLPQIEEYFDRAKYIYLYRDGRDVALSFRKAVVGEKHFYH
                     IAREWDEAQRLALDFRSSVPAERFFNLSYEALTAEPEKTARALCKFLGVSYRPAMLEF
                     YKSGEAKRAASASNLWENVTNPVMRQNTGKFMKEAREEDIRIFESVAGESMDRLGYGR
                     MFARKGEELRFSNEQIAQFDAENRRLKEELRKSIDPADLKRRGVQEGLIKEIAARGAK
                     AAALG"
     gene            complement(7069..8268)
                     /gene="chrA"
                     /locus_tag="VI319_10685"
     CDS             complement(7069..8268)
                     /gene="chrA"
                     /locus_tag="VI319_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY6864353.1"
                     /translation="MKKERTRKSKPVPSLLWLFLDFLKIGATAFGGFMALISVVQHQI
                     VDRRRLLSQREMLDGISLATILPGPVAVNVVAYTGYKVRGALGAIVSAVAVILPSFVL
                     IVVLSYAYFRWGQLPAVSKAFMGFVPAVTAVIVHAAWGMARKAKLGPPELTIATVAFL
                     ALAMKGGFYVTLIVVAASGLAGWLLFSRTPAATRPETGRRGDSPKPRPARLLAVEGPV
                     LAAALLAFDTGVAAKLFFTLAGMSVLLFGGGFVFIPLIEHIVVQEHSWLTHQEFVDAI
                     ALGQVTPGPILISAAFIGYKLMGLAGAAIATVAIFLPPAVLMIFGTKALARARETLTV
                     QAALRGIRAGVVGMVAAAAWSVGSTAVMGWQSALIFAAVLTALFRFRIDVAWVIPSAG
                     LIGLMLY"
     gene            complement(8287..9156)
                     /locus_tag="VI319_10690"
     CDS             complement(8287..9156)
                     /locus_tag="VI319_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY6864354.1"
                     /translation="MIANSIFGMVLGLALGWGTSALAQKNGASSGDAKGRLILAINEG
                     AAGNVTATDIAFRYEQFKQIVEKAVGAPVTLVAVRDARTLRKSVQTSAFALILSRPAD
                     VLAEAVRDYGYQAIVVSQEPAHALFIVKKESPLKTIGDVKGKSILTPDRYAYMWRIAN
                     AMMRDNKISMAHEQVRAMSDQAAIGWSMEGGFFDVGVVASFSGVGRTWEKNGGRVIAR
                     SPELPNTPMIASQKISPAQIQKLRGALIALRNSESGAAVLKDIGVPGFKEASSQSLID
                     LITWLGDLETPKE"
     gene            complement(9712..10107)
                     /locus_tag="VI319_10695"
     CDS             complement(9712..10107)
                     /locus_tag="VI319_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864355.1"
                     /translation="MYSFTRSRQLIAVWLLFGLPALIPASSDAAQRQAGLSQVALSLE
                     RTIPTLVSSQDYRSASSTAFLLATARSRLDDSAAACAALSQSLEYYRMAIAQDLGGRF
                     DGRGRGIDEGSDGMAVIRAKFGCDRPHSA"
     gene            10344..11189
                     /locus_tag="VI319_10700"
     CDS             10344..11189
                     /locus_tag="VI319_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY6864356.1"
                     /translation="MKLTSEQLSRFEDQGYLFFPGLFSAEEIQVLADEVPRLYVQRRP
                     ENVREKTNDELVRTNFAAHLYSYPFAKLARHPRMVEPVKQLFGEDVYMHQFKINGKLA
                     FDGDVWQWHQDYGTWKNDDLMPEARAMNVAIFLDDVNEFNGPLMFIPGSHKLGVIDAA
                     HDVTTTSYPLWTINDDTIRKLVERGGIVAPKGKAGSMIVFHSCLVHASTSNLSPWNRV
                     SVYLSLCAVSNHIRQFKRPEYIAHRDFAPIRCLPDDCLIKDYDVALPWKNGTPAEALK
                     PQMAA"
     gene            11200..12558
                     /locus_tag="VI319_10705"
     CDS             11200..12558
                     /locus_tag="VI319_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6864357.1"
                     /translation="MNLHGKLCEREAQGKPLRVGVIGAGKFGAMYIAQVPQTPGVHLV
                     GIADLSPPNACANLARVGWKPEMYGASSLDAAVRERRTHVSDDWEALVAHPAIDIVIE
                     ATGNPPAAVVHALTAFGRGKHVVMVTVEADAFCGPLLAQKAREAGVVYSLAYGDQPAI
                     ICDLVDWARAAGFPVVAAGRGHKWLPHFAQSTPETVWEYYGLTPEQARVGGLNPKMFN
                     SFLDGSKPAIESTAVCNATGLTPAPDGLTYPPASIDALPSVMRPRSEGGQLHHKGQVE
                     VVSSLERDGRAIPYDIRFGVWVVFEGETEYVRRCFAEYGVRTDPSGRYASMYKRWHLI
                     GLEVGISVASVGLRLEPTGCATGFRADAVATAKRDLRAGEMLDGEGGYTVVGKLMSAE
                     DSIRAGALPLGLAHQLKLSNPVRQGQPVRWADVSVDANDSAIRARREMEALFGASRKR
                     AA"
     gene            12758..13795
                     /locus_tag="VI319_10710"
     CDS             12758..13795
                     /locus_tag="VI319_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY6864358.1"
                     /translation="MIIDDQSTGRLVLSEIIRGLDNSIEVVTFSEPVEAIEYARKHGV
                     DMVLTDYKMPNMDGIEAIRRLRRIFGYEDIPIVCVTVVSDREVRYRALEAGATDFLIR
                     PIDRLECGARCRNLLSLRRHEILKKNRTRILEQKIDEATRELRRREVDTLFRLARAAE
                     QRDKVTGMHLVRMAKYSSLLARGLGLSVAEQDAIELAAPMHDIGKIGIPDRILQNPGS
                     LSGEDLRTMQSHARIGYDLLKDSSSGYLQMGAVIALGHHERFDGSGYPNGLKGEHIPL
                     EARIVALADVYDALTSVRPYKPAWPVSDALTFVEDQRARHFDPKLVDILLDNRDEVLA
                     IGEKFADQAVQ"
     gene            13815..16328
                     /locus_tag="VI319_10715"
     CDS             13815..16328
                     /locus_tag="VI319_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6864359.1"
                     /translation="MKPFLSAVRGRGRFSGTAGSEHEQAIVRLFVGLVLFIYLLPQAF
                     HASIASASTDMLFMVAMVLYLLCATVIFGWIHLSPKPSALRPVCAAVLDMGAVTFFMY
                     NTGQYGAPLYCVYLLTVFGHGFRYGIAYLYNSLILAAAGFTLVLFSNPYWIANQTLGI
                     GLLVGMIVLSLFVGKLVTRLFDSLRREEAANQAKRRFLSTVSHEMRTPLNAIIGMNDL
                     LMDTSLNPEQTEMARAMHDASRSMLKLVEDVLDISKIEAGKLTLEETDFDLHGVINST
                     TSVLTHQAELRKLYLTTHIMPEVPHALRGDPYHLRQILYNLIGNGIKFTESGGVTLTV
                     SSKGESDHVVRLHFAIRDTGIGISPDAQDRIFESFTQADDSTTRRYGGTGLGTTISKQ
                     LVEMMGGQIGLQSEAGKGSTFWFELPLKKQVSTQDLENTFRLNDIRVMLLRFTEEQYE
                     PLERDISTWGAQTSRAPSPESAAARIAEAQSLGRPYHLAMVHDDGGDIAATLAAVRGN
                     RAKLPAVLCVDHVDDARRAEALTAGYVSVLQIPCEKRLLFNAIHAVSTAQSGREGVIS
                     LSDYYAARDTNRRRHQVLVAEDNPTNQRVIRGILERAGHQVRLAANGQEALDILENER
                     FDVLVMDFNMPELGGLDVVRAYRFIDPEAAEVPVIMLSADVTPDAMKECREAGVDTFL
                     AKPIEARRLLDEIAALLAKRNAARSSASAKVEPGDAVVLNTKTISDLELIGSGGRFMT
                     ELVNGFLQDGEALLRQMETAMKDGNFETLRDIMHAMKGSAATLGTDLLFRTCSDITAL
                     TRSELQAAAPHVLRNVQDQFQQARMALLEYLKKSQTLAR"
     gene            complement(16370..17293)
                     /locus_tag="VI319_10720"
     CDS             complement(16370..17293)
                     /locus_tag="VI319_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY6864360.1"
                     /translation="MNARVDFGLSPSPFEKKYHQIETRFDPEHAIAWTYLNPAGAPCF
                     NPEMLEELRAHDAAIQSSGGRLLHGTELHQIRYYVGASRSENVFSLGGNLNILLPLIR
                     SGDRETLMHYSKLCIDALFPRICAYHSQIITISLVQGEALGGGFETALSSGVIVAEKR
                     ARMGMPEIMFNLFPGMGAYSLLARRVGMRRAEEIILSGRIYTAAELHEMGVVDVLVED
                     GTGESAVYDYVRQNERRRNGLQAVLECRQYVNPISYEELLKITTLWVDAALRLEEKDL
                     KLMSRLARSQLKRGAKPLVSEPVREPELATA"
     gene            complement(17484..17559)
                     /locus_tag="VI319_10725"
     tRNA            complement(17484..17559)
                     /locus_tag="VI319_10725"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17524..17526),aa:Asn,seq:gtt)
     gene            complement(17668..18882)
                     /locus_tag="VI319_10730"
     CDS             complement(17668..18882)
                     /locus_tag="VI319_10730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEY6864361.1"
                     /translation="MNAPHSPSPLASVELAPRDPILGLTEAFLADKNPRKVNLGVGVY
                     YDDEGKVPLLECVRRAERMLVEQAAPRGYLPIDGLPAYDRTVQRLVFGADSRALKEGR
                     IVTVQALGGTGGLKVGADFLRRLAPGAEAWISDPTWENHRGLFENAGFTVRAYPYYDA
                     KTHGVDFAAMMTTLKSLPAGTVVVLHACCHNPTGVDLSGEQWDAVIDAVVARRLLPFL
                     DFAYQGFGDGIEQDASAVRRFADLGIPVFVSSSFSKSLSLYGERVGSLSVVAPTADEA
                     NRVLSQLKRLVRTNYSNPPTHGGRVVATVLTTPELRALWERELTQMRDRIKEMRAALV
                     ERLRQRAPRADFGFVLGQRGMFSYSGLSREQVVRLREEFGVYAIESGRICVAAVNSRN
                     IDYVAESIAKVL"
     gene            18931..20994
                     /gene="uvrB"
                     /locus_tag="VI319_10735"
     CDS             18931..20994
                     /gene="uvrB"
                     /locus_tag="VI319_10735"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY6864362.1"
                     /translation="MIVTFPNSPFQLEQAYVPAGDQPEAIDKLADGVRDGLSYQTLLG
                     VTGSGKTYTMANVIARLGRPALVIAPNKTLAAQLYSEFRQFFPGNAVEYFVSYYDYYQ
                     PEAYVPSRDLYIEKDSSINDHIEQMRLSATKSLLERRDTVIVATVSCIYGIGDPVDYH
                     GMILHLRDRERYAQRDIIARLAAMQYERNELDFRRGTFRVRGDVLDVFPAEHSETALR
                     IALADDEIDSLTLFDPLTGQTHHRVPRFTVYPSSHYVTPRATTLRAVEAIKVELAQRI
                     DHFHKAGKLLEAQRIEQRTRFDLEMLAELGFCKGIENYSRHLSGRNEGEPPPTLIDYL
                     PQNSLMFIDESHVTLGQLGGMYRGDRSRKENLVDYGFRLPSALDNRPLRFEEFERMMP
                     QTIFVSATPADYERERSGQVVEQLVRPTGLIDPQLSIRPASTQVDDLLSEIHLRVARQ
                     ERVLVTTLTKRMAEELTDYLAEHGVKVRYLHSEVETVERVEIIRDLRLGEFDVLVGIN
                     LLREGLDLPEVSLVAILDADKEGFLRSERSLIQTIGRAARHINGMAILYADRVTDSMK
                     RAIGETDRRRAKQVSFNEKHGITPIGITKQVRDMIEGVYDAREVEGELKAAQAQARYE
                     SMSEKQIAKEIRQLEKRMLEFARNLEFEKAAQARDQLAELKKSFFGASPEDEDEPGRR
                     AGASG"
     gene            20994..21500
                     /locus_tag="VI319_10740"
     CDS             20994..21500
                     /locus_tag="VI319_10740"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY6864363.1"
                     /translation="MSGASDRPVRVLFVCTGNICRSPTAEGIFLKLVHDAGLDQEIRV
                     DSAGTHGYHVGEPPDLRTCEAAAHRGYDLSALRARKFERADFHSFDLVLAMDRENHSH
                     LVRLVPPSEGHKLKMMMEYSRRFSGREVPDPYYGGSRGFEVVLDMLEDASRGLLEAIV
                     EKQRAPGP"
     gene            complement(21525..24242)
                     /locus_tag="VI319_10745"
     CDS             complement(21525..24242)
                     /locus_tag="VI319_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY6864364.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLIDLDIESAAKEQRKSNIYKG
                     VITRVEPSLEAAFVDYGTDRHGFLPFKEVARQYFKDGIDVNRARIQDAIAEGQELIVQ
                     VEKDERGTKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRNELRDLMDQLQVP
                     QGMSIIGRTAGIGRTLEELRWDLDYLLKLWDKIVEATEPMKDGDGKLLNPAPFLIYQE
                     GSLVIRAIRDYFQPDIGEILIDTDAIHEQTLAFMGNVMPDNVNRVKRYRDDVPLFSRF
                     QIEHQIETAYSRQVSLPSGGAVVIDHTEALVSIDVNSARATKGHDIEQTAFQTNVEAA
                     EEIARQLRLRDLGGLIVIDFIDMENQRNQRDVENKLRDALRFDRARVQMGKISRFGLM
                     ELSRQRLRPSLGEGSNITCPRCNGTGHIRGTESTALHILRIIQEEAMKENSASVHAQV
                     PVDVATFLLNEKRSDIHSIEARLKVNVVLIPNVHLQTPNYHVERLRHDDLNQADAIPV
                     SYNLVSVPADEELSSAAAPEQRAPRQQAVVQGVTPEQPAPVPAARPQPVPQPQARSGP
                     ALGEAGSGIIDRIFGWFRREQPAASAVVSEPRPVQSAAQREPRPEHNRDRRDRRDRGD
                     SDRFRNRGEGRPEQRPGREERREGRPDRPPRQERDRQQRSDRPQGENRPPREPQQRLE
                     RGEQPQAEQARPPQAERQGQAAPQGPGHGHDGERERGERGGRRRRRGGRDRGDRGERR
                     EQPARSERRPDQAGNPTVDPATAAAGSQAIRDFQRAVHAAELAAASDSEVDAHQPARQ
                     APRSVHEPEAPPVAREAEDQPVVHEIEARPVVRERAPEPPAPAQDPAEIERALERSGL
                     ELVQTKPGVQAEVPVESEFVPARRERRPPPAELNQPLVQVETRKEDAPS"
     gene            24605..25543
                     /locus_tag="VI319_10750"
     CDS             24605..25543
                     /locus_tag="VI319_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY6864365.1"
                     /translation="MKDLSNASAVAVEVDAGSEGQRVDNFLLRVCKGVPRSHIYRVLR
                     TGEVRVNSRRVGATYRLQLGDRVRIPPIRVAPATPRRAPPVELPIVFEDDSLLVVDKP
                     SGIAVHGGSGVSFGVIERLRSARPQARFLELAHRLDRETSGLLMLAKRRTALVALHAA
                     LRDGRVRKRYLVLVKGRWRDAERSVDLPLRKSISASGERRVSVDRSGREALTVFRRVR
                     EVPGYTLLEAELKTGRTHQIRVHLAHLGFPIAGDDKYGDFELNRALAKRGLKRMFLHA
                     AELELAHPVTGAALSLTSPLPRELHLFLDSVQREVQ"
     gene            25540..>25955
                     /locus_tag="VI319_10755"
     CDS             25540..>25955
                     /locus_tag="VI319_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY6864366.1"
                     /translation="MSARRYDLLVFDWDGTLIDSAPTIVACIQSACRDLGLAVPDDER
                     ASHVIGLGLRDALAHAVPGLDPDEYGRVVERYRHHFLQRDPHMALFDGTREMLHGLRD
                     AGHVLAIATGKSRAGLARALESTRLGPLFSASRCAD"
BASE COUNT         4602 a         8324 c         8442 g         4587 t
ORIGIN      
        1 gatgcccgtg ccgatgatga acgtcatcaa cggcggcgcg cacgcgaaca acagcctcga
       61 catccaggag ttcatgatcg tgccggtcgg cgcgtcgacg ttccgcgacg ccctgcgctg
      121 cggcgccgag atcttccagg cgctcaagaa gctgctcgac gcgaaaggcc tgtcgacgac
      181 ggtcggcgac gaaggcggct tcgcgccgag cctgccgaac aacgaggccg ccatcaagct
      241 cgcgctcgag gcgatctcga aggcaggcta ccagcccgga accgacgtgc tgctcgctct
      301 cgactgcgcg agctcggagt tcttcaagga cggcaagtac gtgctcgagt ccgagaacca
      361 gtcgctcgcg ccggggaagt tcgcggacgt gctcgcctcg tgggcggaca agtacccgat
      421 cgtcagcatc gaggacggca tggccgagtc cgactgggac ggctggcgcg gcctcaccga
      481 gcgcctcggc gccaaggtgc agctcgtcgg cgacgatctg ttcgtgacca acaccaagat
      541 cctgcgcgaa ggcatccagc gcggcatcgc caactcgatc ctgatcaagg tcaaccagat
      601 cgggacgctg accgaaacct tcgcggccat cgagatggcc aagcgagccg ggtacaccgc
      661 ggtcatctcg caccggtcgg gcgagaccga ggacacgacg atcgccgaca tcgccgtcgg
      721 catgaacacg ctgcagatca agaccggatc gctctcgcgc tccgaccgca tcgccaagta
      781 caaccagctg ctgcggatcg aagaggatct cggggacgcg gtcggctatc ccgggcgcgg
      841 ggccttctac cagctgagat gagaccgctc gccgccgtcc tggccgcgct cgtgctgctg
      901 cttcagtacc cgctctggct cgggaagggc ggctggctgc gcgtctggga tctcgaccgc
      961 caggtgcagg cgcagaagga ccagaacgcg aagctgcggc tgcgcaacga ggcgctcgac
     1021 gccgaggtgc gcgacctcaa gcagggtctg gatgcgctcg aggagcgcgc gcgctacgag
     1081 ctggggatgg tcggctacga ggagacgttt tttcagctcg tgccgcgtcc gtccgacgcg
     1141 cgcaaatgag ctgatccgtt tcgggccggg ctagcgcgtg cggaaacgcg cttcgaagcg
     1201 cgcggcaaag ccctcgacgt ctccgtcggg aagatgattg atgcccccgg cggacttgcg
     1261 gcctccgcca gtcgggaaat ccttgcagaa atcggcggcg ctcacggcgc tggtcggagg
     1321 cacgcggacg ctcaccgtgt atccgccggc ggatttcggc gagaggattg cgagcgccac
     1381 gttggggcgc tcccgggcga gctcgttgat gagaacgccg atggcgcgcc tcgcccaggg
     1441 cgcgttcggg aggaccacga tgccgctcgg tccgtagacc cgggcggcgg cgcctcgcgc
     1501 cttcgccatg tcgtcctcgt aacccgcgcg caacttctcg tagacgggcg cgtcggtcat
     1561 gaaggcgaag gggtccgtgt agggcagcag gcgcgcggaa agctccgccg ggttgaagtg
     1621 gagatcctcg accgattcgc cataagcgtt gtagttcagg catacgccca acctctccag
     1681 atccgtcgcc tgggcttccg tgagcccggc ctcctcggcc atggcgcgcg ccacggtggt
     1741 gaggttgtcc ccgaaggctg ccaccaccgc ccagcgcctg taccgacctt cgagatgacg
     1801 gtcgaccagg atgctggtgc acacgtcggc cgactcgtcg atgtgggcat cgagcccggg
     1861 atgagcgggt atcgcgccgg cgcggtgatg atcgaagtag gtcacgcgga tgcccgaatc
     1921 gagcaggcgc atgagctccc cgcggttctc gtgcagcgag acgtcgagga cgaagactcg
     1981 atcgccggcc gggggcgcga ggtgcctcag cagcgtgatg tcccgtttca ccccggtgac
     2041 caggttcgcc ggccccggtt gcgccagtcg cagctgctgg acggcgcaaa gtccgtcggc
     2101 gtcgccgttg aatgcgaagt gacgcaatct ccgggcgtgc gcgttttcgc cgagcgtcag
     2161 cgcccctcgc tggcgatggg aggtggcaag ggcgatcggc ttcagatcca ccgtggccgg
     2221 aggcaggccg acggcgcgcg gattttcggg cttcgccttg aaaaaaatgg acagcaggta
     2281 catgtccagc agcatggccg tggccatcgg gccataggcc agtggcgcct gcgcgatctg
     2341 cagctcgatg agcacccgcg acaggccgaa cccgacgacc cacagatcga acgtcatgca
     2401 aatgcgcctg aacgtctccg ggttcaggcg gcggaaagcc aggaagccga ggggaatgcc
     2461 gagcagcacg ccggggacga tgagcgccgt ggtccccagc gcctggttgg tgaacaagcc
     2521 caggtgcagg tatgcgatgg ccgtgaagga agactcgacg accctgatga ggcccaggcc
     2581 ggcacggaaa tcgcgcttca cgaggccctg gttgttgaag aacagcgcca ggggcgggcc
     2641 tgaaatcgtg ctcaccgaat acaggaaccc caggcccgcg ccgaagggcg cggccaccag
     2701 acgctcggag ccgatcggtc tcctgatccc ggacgcctgg agaaggatca agggcaggag
     2761 gaacgcgtac gtcgcgaact tgagccagcc cggatgaatc gaggcgagaa agtagcttcc
     2821 cgccgccacg ccgggcacga ggccgaacaa tatcggatag acgcgccgcc agaccttcgg
     2881 gatgctggcc cggttcatga acaagacgta gatgttcacg aaaacttcga tcagcacgag
     2941 cgcgggattg aggatgcggc tcgtatagaa cagcaggccc accggcaccg tgagcgacga
     3001 aaatccgtac ccgagcgcgc cgttcacgaa ggcggcgaag aacgtgatcg cgatcagggc
     3061 gatggaggcc gtatcgaggg gcggcatgat cgagagctcg ccggtgatcg ccgctcagaa
     3121 atagccttcg cgcttcttgc gttccatggc gccgacctgg tctttgtcga tcagccggcc
     3181 ttgccgctcg gagtcgggag aggcttcgag ctcggcgatg atctggtcga gcgtcgtcgc
     3241 gtgcgagacc accgccgcgc tcagcggata gcatcccagc gtacggaacc ggatcatgcg
     3301 gtcctccggc gcctcaccag gagcgagggg cagccgctcg tcgtcgacca tgatgatcat
     3361 gccgtccctg atcacggtcg gtcggcgctt cgcgtagtac aagcccacga gcgggatccg
     3421 ctcccggcgc acgtacttcc agacgtcgag ctcggtccag ttgctgagcg gaaacacgcg
     3481 catgctttcg ccggcgccga cgcgcgcgtt gtacagcctc cagagctcgg gacgctgatg
     3541 tcgaggatcc cactgatgat gggtgtcgcg atacgagaaa atccgctcct tcgcccggct
     3601 ggcttcctcg tcgcgccgcg cacccccgat cacgcagtcg tagcaaccgg cttcgagcgc
     3661 ctggcgcagc gccagcgtct tcatgatttc cgtatggacg cggctgccgt ggtcgatcgg
     3721 attgatcccc tgcgcgagac cgtcgggatt ggtgtgaacg aggagattga agccgagacg
     3781 tctcgcttcg gtgtcccgga acgcgatcat ctccctgaac ttccacttgg tatcgatgtg
     3841 aaggacggga aacggcagcg gcgcgggaaa gaacgccttg cgcgcgaggt ggagcagggc
     3901 gcacgagtcc ttccctatgg aaaagagcaa tacgggccac ttggcctgcg ctatggcctc
     3961 gcgcagtatg tagatggcct cggcctcgag ctcgtcgagg tagtcgtcgc tcttttcaga
     4021 catggatgcc tcgcgctttg agcgacacga ggatgctctc gacgctctgc gcgacggtgc
     4081 tggtggacgt gtcgatcctc acatcggggc tcgaaggcgg ctcgtaggcc tgcgaaatgc
     4141 ctgtgaactc gggcagttga ccgcgccggg cccgctggta caggcctttc ggatcacgct
     4201 gctcgcatac ggcgagaggc gtatccacga agatctcgag gaattcgccg tcggcgacca
     4261 gctcccgtac gagatcgcgc tctcgccgga acggcgatat gaatgccgtg atcacgacca
     4321 gccccgcgtc gagcagcagc ttcgcggttt ctcccacacg acggatgttc tccgtcctgt
     4381 ccgcttcgga aaatccaaga tcgcggttga gcccgtgacg gacattgtcg ccgtcgagca
     4441 gcatcgtgtg cacgcccgcc gcgttcagcc tcgcctcgag ggcgttcgcg atggtggact
     4501 ttccggcgcc gctcaggccg gtcagccaga tcacctgcgg cgagtgaccc ttgatggatg
     4561 cgcgtgccga ccgggtgacc gcggtctcgt gccacgaaat gttcttggcg gcgaccgtcg
     4621 ataccatcgc ggcgccttcc gtccggaacg tcgtccggtc gatgaatatg agcgatccgg
     4681 tctggaccga gtcggcatac gcgtcgaccg ggacggagcg ttcgagaacg atctcgcagg
     4741 tgccgacgtc gttgcacgcc aggcgcgatg cctcctgcga cccgagcgtc tcgacgtcga
     4801 gcttgtggat gatgcggcgc acggaacccg ggacgatcgt gcatccggcc tggaaatcgt
     4861 atcggcgacc gggcaccagg ggcgtgtcgg acatccagac cacgttcgcc tcgatgtggt
     4921 cggtcaccgc cggcgggaca tcggtcgcct tgaccagcca gtcgccgcgg gcaacgtcca
     4981 gctccttgtc caggacgacc gtgactggct ggccggggcg ggcgatctgg agatctccgg
     5041 cgaaagtgca aagccggtcg atcctggcgt agatgccgga gggcagggcg cggacggtgt
     5101 cgccgacgcg aaggggagcg ccgagcacct ctccggcgat gccgcggaaa tcctggttcg
     5161 gccggttcac ccattgcgcg acgagccggg ccgagcagct cggcggccgg cggttcggca
     5221 tggcctccag ggcttcggaa atcgtgagcc cggcatacca cggcgtctcc gccgccttgc
     5281 ggacgacgtt gccgccgtgc gtggcgctgg ccgggatgac ggtcacgtcg ctcagcccca
     5341 gcttgtgcgc gtactcgagc gcttcccgtc ggatggcgtc gaagcggaca cggtcccaat
     5401 cgaccaggtc catcttgttg acggcgatca ataccgaccc gacccgcatc atcgccacca
     5461 cggacagatg gcggcgggtc tgcgccagca cgcccttgcg ggcgtcgatc aacacgagcg
     5521 cgccgtcgca gtgcgaggcc ccggtcacca tgttccgcgt gtaatgctcg tggccggggc
     5581 agtccgcgac gatgaatttc cgattcgggg tcgcgaagta ccgccacgcg atgtcgaccg
     5641 tgatgccctg ctcgcgctcg atgagcaggc catcgagcag gagcgagtaa tcgggattgc
     5701 tgcgggagaa gttgcgccgc tcgctttcgc ggaggacgct ttcccaggtg tcctccggaa
     5761 cgagcgaaag gtccttgaga agacgcccga tcaggctgct cttgccgtca tcgacgctgc
     5821 cgcacgtcag aaacctgaag atccgtgcgt tcgatatgtc tttggaccga ccggcgtgct
     5881 gcctgccacg cctggtgtgt gcctcggata caccatcccg accgtttttc ctgacgactt
     5941 gctgatcacc gtatgccacg tgaacctcat cgagggggtg cgattgcgca atatcggagc
     6001 gtgtgtgcgt gcatgcgcgc ggtcatccca gggctgcggc cttcgcgcct cgcgcagcga
     6061 tttccttgat cagcccctcc tggacgccac ggcgcttcaa gtcggccggg tcgatgctct
     6121 tgcggagctc ttctttcaac cgcctgtttt ccgcgtcgaa ctgggcgatc tgctcgttgc
     6181 tgaagcgcag ttcctcgcct tttctcgcga acattcgtcc gtacccgagc cggtccatcg
     6241 attcgccagc aaccgactcg aaaatgcgga tgtcttcttc gcgggcctcc ttcatgaatt
     6301 tgccggtgtt ctgccgcatc acgggattcg ttacgttttc ccagagattg ctggccgagg
     6361 cggcccgctt ggcttcgccc gatttgtaga actcgagcat ggccggccgg tagctcaccc
     6421 ccaggaactt gcacagggcg cgcgccgtct tctccggctc cgcggtcaac gcctcgtagc
     6481 tcaggttgaa gaatcgctcc gccggcacgc tggaccggaa atcgagcgcc agacgctgcg
     6541 cttcgtccca ttccctggcg atgtggtaga agtgcttttc tccgaccacc gctttacgga
     6601 acgaaagagc gacgtcccgg ccgtcccggt acaagtagat gtacttcgcc cgatcgaagt
     6661 actcctcgat ctgggggagg taatagacgt tcgcgaggct cttgcagcac cacgtcttgg
     6721 ccttccacgc ctccgccatg atgtcgtata ccgcaccgaa aaccgcgacc aggctgtgct
     6781 tcctgcagcg agcggcgacg tccttgcgat cgagaacgac gccttcccac ggcacgggat
     6841 tcaactcgac gagcctgcag acgtcgtcga ccagcagaga gaacgactgc cccttgccca
     6901 gatcgccgta atagggaacc aggggcatca ttcgctggag gatgtgcggc ggatgcggcg
     6961 cggcgatctc gggcagctca ttgagcatca gtcgcagcaa gttcgatccg gaccgctgta
     7021 tcccaatcat gaaaattgcc atatgtcttc ttgcgcctcc tcagtgcctc aatagagcat
     7081 caagccgatc aggccggccg acggtatgac ccacgcgacg tcgatacgga accgaaacag
     7141 ggctgtgagt accgcggcaa agatcagcgc cgattgccat cccatcacgg cggtcgagcc
     7201 tacgctccag gccgccgccg ccaccatgcc gacgaccccc gcccgtattc cacgtaaggc
     7261 cgcctggaca gtgagcgttt cccgggcccg ggccaacgct ttcgtgccga atatcatcag
     7321 caccgcgggc ggcaggaaga tcgcgaccgt cgcgatggcc gctcccgcca gccccatcaa
     7381 cttgtagccg atgaaggcgg cgctgatcag gatcggcccc ggcgtgacct gcccgagtgc
     7441 gatcgcatcg acgaattcct ggtgtgtcag ccaggagtgc tcctgcacca cgatatgctc
     7501 gatcagcgga atgaacacga acccgccgcc gaacaggagc acgctcatcc ccgcgagagt
     7561 gaagaaaagc ttggccgcta cgccggtgtc gaacgcaagc agggccgcgg cgagcacggg
     7621 gccttccacg gccaggagcc tcgccggacg cggtttcggc gaatcgcctc gtcgaccggt
     7681 ctcggggcga gtcgccgccg gggtgcgcga aaacaagagc cagccggcga ggcccgatgc
     7741 ggcaacgaca atcagcgtga cgtagaaacc gcctttcatc gcgagcgcta ggaacgcaac
     7801 cgtcgcgatc gtgagctccg gtggtccgag cttcgccttg cgcgccatgc cccaggccgc
     7861 gtgaacgatc acggcggtca cggcgggaac gaatcccatg aatgccttgc tgaccgcggg
     7921 aagctgtccc cagcggaaat aggcgtagct caggaccacg atcagaacga acgacgggag
     7981 gatcacggct accgcggaga cgatggctcc caacgcgccg cgcaccttgt atcccgtgta
     8041 tgcgacgaca ttgaccgcga cggggccggg aagaatggta gcgagcgata tgccgtccag
     8101 catctcgcgt tgggacagca gtcttcttcg gtcgacgatc tggtgttgga cgaccgatat
     8161 cagcgccatg aatccgccga aggcggtcgc gccgatcttc aggaagtcaa ggaacagcca
     8221 gagcagagaa ggaacgggct ttgacttgcg ggtccgttcc ttcttcatgg ttcaggcgtg
     8281 ttcccactat tccttgggcg tttctaggtc gccaagccag gtgatgagat ctattagcga
     8341 ctgcgaggaa gcttccttga accccggaac accgatgtct ttcagcaccg cggccccgct
     8401 ctcgctgttg cgaagcgcga tgagcgcgcc gcgcagcttt tgaatctggg cgggcgagat
     8461 cttctgcgac gcgatcatcg gtgtattggg cagctcgggg ctgcgcgcga ttacccttcc
     8521 accgtttttc tcccaggtgc gcccgacgcc cgaaaaggac gcgaccacgc cgacatcgaa
     8581 aaagccaccc tccatcgacc agccgatggc cgcctgatcg ctcatcgcac gaacctgctc
     8641 gtgcgccatc gaaatcttgt tgtcgcgcat catcgcgttg gcgatgcgcc acatgtacgc
     8701 gtagcggtcg ggtgtcagaa tgctcttgcc cttgacatcg ccgattgtct tgagagggga
     8761 ctcctttttc acgatgaaaa gcgcgtgcgc cggctcttgc gatacgacga tcgcctggta
     8821 gccatagtca cgcacggctt cggccaatac gtcagcgggc cgtgacagga tgagcgcgaa
     8881 ggctgacgtt tggacggact ttcgaagtgt ccgcgcgtca cgcacggcaa ccagcgtgac
     8941 gggagccccc accgctttct ccacgatttg cttgaactgc tcgtatcgga acgcgatatc
     9001 ggtcgccgtc acgtttcctg cggcgccttc gttgatcgcc aggatcagcc gacccttcgc
     9061 gtcgccggag gaggcgccgt ttttctgtgc tagagcgctc gtgccccagc cgagcgccaa
     9121 tccaaggacc atcccaaaga tcgagttcgc aatcattctt atcattttgc tgctcccatt
     9181 gatcgaccgt ggccggccat atgatgtgag catatgacaa cgcgtttctg agatgcatca
     9241 agaaaagccc ggtccacgct tcagagattt cgcatcacat gcaatacgtc acgtaccttg
     9301 acgagttgga ttgtcgcgat ggcgcggcat tgagaaatgc aaagtgtccc gaaacgagcg
     9361 ggcaacgaag acgcatgtcg cgaaccaaaa tgaacttctc aatgcaatgg cctgtaatcc
     9421 ttcaccaaaa acccgacgcc gcaactccga cgtaggatgt tataaaccgc gaggcgcggt
     9481 caatcctgta gatagtgaca gctgtaggaa gtgacaggga tcggcaacga gagagatgaa
     9541 tcgcaagcgg aaaagcgtgc gacgtgaaca tgcagaaaaa ttcacatcga cgttttgatg
     9601 caaagaccgg gccggtatcg actgttcgcg cggcgtttgc gagaccgtcg cgaaagaaaa
     9661 acggcttcgc gccgttcggc gcgaagccgt tttcgagagg ttcagccgac cttaggcgga
     9721 atgcgggcgg tcgcagccga acttggcacg aatgaccgcc ataccgtcac tgccttcgtc
     9781 gatgccccga cccctgccgt cgaatcgacc gccgaggtcc tgggcaatcg ccatgcggta
     9841 gtactccaga gattgcgaca acgcggcgca cgcggcagcg ctatcatcca ggcgcgagcg
     9901 cgcggtagcc agcaggaaag ccgtgctgct tgccgagcgg tagtcctgag acgataccag
     9961 cgtcgggatg gttctctcca aagatagggc gacctggctg aggccggctt ggcgttgcgc
    10021 cgcgtccgag cttgccggga tcagggcggg caagccgaac agcagccaca cggcaatcaa
    10081 ctgacgcgac cttgtgaagc tatacatagt ctcctcctga aagtttaagt tcgaggctgt
    10141 gttgaggtca cgacacaaac gtcaatctgc ctggtgtttc gccgtctgtg tcgagccccg
    10201 cagcttcggc ctatgtaggg ccttgcctga caacaccacc ggaaccttcc tacgttgtag
    10261 caattgagat gccaggggcg cgtaccgcac tgtcatataa tcacggtcca gccgttaata
    10321 cccgtgccga ttcaggaacg cgcatgaaac tgacctccga gcagctctcg cgattcgagg
    10381 atcagggcta cctcttcttc ccgggcttgt tctcggccga agagatccag gtcctcgccg
    10441 acgaagtccc gcgcctctat gtgcagcgcc gccccgaaaa cgtccgcgaa aaaaccaacg
    10501 acgaattggt tcgaaccaat ttcgcggccc acctctacag ctatccgttc gcgaagctcg
    10561 ctcgtcaccc gcgaatggtg gaaccggtca agcagctgtt cggtgaagac gtgtacatgc
    10621 accagttcaa gatcaatgga aagctggctt tcgacggcga cgtctggcag tggcatcagg
    10681 attacggcac ctggaagaac gatgacctga tgccggaggc gcgtgcgatg aacgtggcca
    10741 tcttcctgga cgatgtcaac gagttcaacg gcccgctgat gttcataccg ggcagccaca
    10801 agctcggcgt catcgatgcc gcgcacgatg tcaccaccac cagctacccg ctgtggacca
    10861 tcaacgacga caccatccgc aagctggtgg agcggggcgg catcgtcgct cccaagggga
    10921 aggccggttc gatgatcgtg ttccactcgt gtctcgtgca cgcatcgacg tcgaatcttt
    10981 cgccgtggaa ccgcgtgagc gtctatctca gcctgtgcgc ggtgtccaat cacatacgcc
    11041 agttcaagcg cccggagtac atcgcgcacc gcgatttcgc gccgatccgg tgtctgcccg
    11101 acgattgcct gatcaaagat tacgacgtcg cattgccatg gaagaacggc acgccggccg
    11161 aggccttgaa acctcagatg gccgcctgag gccgcagaca tgaaccttca cggcaagctg
    11221 tgcgagcggg aagcgcaagg caagcccctg cgtgtggggg tgatcggggc gggcaaattc
    11281 ggggcgatgt acatcgcaca agttccgcag acgcccggcg tgcacctcgt cggaatcgcg
    11341 gatctctcgc ccccgaatgc ttgcgcgaac ttggcgcgcg tcggctggaa gccggagatg
    11401 tacggagcat catcgctcga cgcagcagtt cgcgagcggc gcacccacgt gtccgacgat
    11461 tgggaggcgc tggtggctca cccggccatc gatatcgtga tcgaggcgac cggcaatcct
    11521 ccggctgccg tcgtgcacgc gctcacggcg ttcggcaggg gcaagcacgt ggtgatggta
    11581 accgtcgagg ccgacgcttt ttgcggaccg ctgctcgcgc agaaggcgcg agaggcagga
    11641 gtcgtctaca gcctcgccta cggcgatcaa cccgcgatca tctgcgacct cgtcgattgg
    11701 gcgagggccg cgggcttccc cgtcgtggcc gccggccgcg ggcacaagtg gcttccgcac
    11761 ttcgcacagt ccaccccgga gactgtctgg gagtattacg gtctgacgcc ggagcaagcg
    11821 cgtgtcggcg ggttgaaccc gaagatgttc aactccttcc tcgacggctc caagcctgcg
    11881 atcgaatcca cggcggtgtg caatgcaacg gggctcacgc ccgcacccga cgggctcacc
    11941 tacccgccgg catcgatcga cgcgcttccg agcgtcatgc ggccgcggtc ggaaggtggg
    12001 cagctgcatc acaagggcca ggtggaggtc gtttcttcgc tggagcggga cggtcgggcg
    12061 attccatacg acattcgctt cggtgtctgg gtcgtttttg aaggcgagac cgagtacgtc
    12121 cgacgatgct tcgcggagta cggagtgcga accgacccct cgggccgcta cgcgtcgatg
    12181 tacaagcgct ggcacctgat cgggctcgaa gtcgggatct cggtcgcgtc ggtggggttg
    12241 cggctcgagc cgacgggctg cgccaccggt tttcgcgccg acgccgtcgc caccgcgaag
    12301 cgtgacctcc gggcgggcga gatgctcgat ggcgaaggcg ggtataccgt cgtcgggaaa
    12361 ttgatgtccg ccgaggattc cattcgcgcg ggcgcgctgc ctctgggtct ggcgcaccag
    12421 ctgaagctgt cgaatccggt ccgccagggc cagcccgtcc gctgggccga cgtctcggtc
    12481 gacgccaatg acagcgccat ccgggcccgc cgtgagatgg aggccctttt tggtgcatcg
    12541 cggaagcgcg ccgcataggc ggcgccgggt gaaaaacgtc acgtcgatca gtcgcttccg
    12601 gaaacgttct cgcaatttcc cctcaaatgg ggtctaattc tccgagtggg atgaggacgg
    12661 agcgccagcg ctccttggga ggacaatgaa gcccaccagc cggtcactca cgctggtcca
    12721 cgacgccggg gaagccaagc tcgcgccgct taccgtgatg atcatcgacg atcagtcgac
    12781 ggggcgtctc gttctgagcg agatcatccg gggtctcgac aacagcatcg aggtcgtcac
    12841 tttttccgag cccgtcgagg ccatcgagta cgcgcgcaag cacggcgtcg acatggtcct
    12901 cactgactac aagatgccga acatggacgg catcgaggcc attcgtcggc tgcgccgcat
    12961 cttcggatac gaggatattc ccatcgtctg cgtgaccgtg gtgagcgacc gcgaggtccg
    13021 gtaccgggcg ctcgaggcag gggcaaccga tttcctgatc aggcccatcg accggctgga
    13081 gtgcggcgcg cgttgccgca acctcctgag cctgcgtcgc cacgaaattt tgaagaagaa
    13141 ccggacgcga attctcgagc agaagatcga cgaggcgacc cgcgagctgc gccggcgcga
    13201 ggtcgatacg ctgttccgcc tggcgcgtgc cgccgagcag cgcgacaagg tgacgggcat
    13261 gcacctggtt cgcatggcca agtactcgtc gctgctcgcc cgcggtctgg ggctctccgt
    13321 ggccgagcag gacgccatcg agctcgccgc gcccatgcac gatatcggta agatcgggat
    13381 tcccgaccgt atcctgcaga atccgggaag cctgagtggc gaggatctgc gcacgatgca
    13441 gtcacacgct cgcatcgggt acgatctgct gaaggacagc tcgtcgggat accttcaaat
    13501 gggggcggtg atcgcactcg ggcaccacga gcgtttcgac ggatccggct atcccaacgg
    13561 attgaaaggc gagcacatcc ccctcgaggc gcgcatcgtg gcgcttgccg atgtctacga
    13621 tgcgctcacg tcggtaagac cgtacaaacc cgcatggccg gtgtccgacg cgctcacgtt
    13681 cgtggaggac cagagagccc gccatttcga tccgaagctc gtggacatcc tgctcgacaa
    13741 tcgggacgaa gtgctcgcca tcggcgagaa attcgccgac caggcagttc aatagccgct
    13801 taccgggagc tgcgttgaag cccttcttgt cagccgttcg cggtcggggc cgcttcagcg
    13861 gcaccgcggg cagcgagcac gagcaggcga tcgtccggct gttcgtcggg ttggtgctgt
    13921 tcatctacct cctgccgcag gcgttccatg cctcgatcgc cagcgcatcg accgacatgc
    13981 tgttcatggt cgcgatggtg ctttacctgc tgtgcgcaac ggtcatattc ggctggatcc
    14041 atctctcccc gaaaccctcg gcactgcggc cggtctgtgc cgcagtcctc gacatggggg
    14101 ccgtcacctt cttcatgtac aacacggggc aatacggggc tccgctgtac tgcgtctatt
    14161 tgctcactgt tttcggccat ggtttccgtt acggaatcgc gtacctgtac aactcgctga
    14221 ttctggcggc tgcaggcttc acgcttgtcc tgttctccaa cccgtactgg atcgcgaatc
    14281 aaacgctcgg catcggtctg ctggtcggga tgatcgtctt gtcgctcttc gtcgggaagc
    14341 tcgtcacccg gttgttcgat tccctgcgcc gcgaggaagc ggccaaccag gcgaaacggc
    14401 gcttcctctc gaccgttagc cacgagatgc gcacgccgct gaacgcgatc atcgggatga
    14461 acgacctcct catggatacg tctctgaatc ccgagcagac ggaaatggcc agggcgatgc
    14521 acgacgcgtc ccggtcgatg ctcaagctgg tcgaggacgt gctcgacatc tccaagatcg
    14581 aggccgggaa gctgacgctc gaggagaccg atttcgacct gcatggggtg atcaacagca
    14641 cgacatccgt cttgacgcac caggcggaac tgcgaaagct ctatctcacg acgcacatca
    14701 tgccggaggt cccgcacgcg ctgcgaggcg acccctacca tctgcggcag attctttaca
    14761 acctcatcgg gaacggcatc aagttcaccg agtcaggcgg cgtgacactc acggtgtcgt
    14821 cgaaaggcga gagcgaccat gttgtgcgtc tgcatttcgc gattcgcgat acagggatcg
    14881 ggatatcgcc ggatgcgcag gatcgcatct tcgagagctt tacacaggct gacgactcga
    14941 caacgcggcg ctatggtggc accggcctgg gcacgacgat ttcgaagcaa ctggtcgaga
    15001 tgatgggcgg gcagatcggc ctgcagagcg aggcggggaa gggcagtacg ttctggttcg
    15061 agctgccgct caagaagcag gtcagcaccc aggatctcga aaatacgttc cgcctgaacg
    15121 acatccgagt catgttgctg cggttcacgg aggagcagta cgagcctctc gagcgggaca
    15181 tctcgacttg gggcgcacag acgagtcgcg ctccgagtcc ggagagcgcc gctgcgcgga
    15241 tcgcggaagc gcagtccctg gggcggcctt atcacttggc gatggtgcac gacgacggcg
    15301 gagatatcgc ggcgactctt gcagccgttc gcggcaatcg ggccaagctg cccgctgtgc
    15361 tttgcgtcga ccacgtcgat gacgctcgtc gagcggaggc gctgacagcg ggttacgtct
    15421 cggtgctgca aataccctgc gaaaaacgcc ttctgttcaa cgcgattcat gcggtatcga
    15481 cggcgcagtc gggccgcgaa ggtgtcatct cgctcagcga ctactatgcc gcccgcgaca
    15541 ccaatcggcg ccgccaccag gttctggtcg ccgaggacaa tccgaccaat cagcgggtga
    15601 tccggggcat tctcgagcgt gccgggcatc aagtgaggct tgccgccaac ggccaggaag
    15661 ccctggacat ccttgaaaat gaacgcttcg acgtcctggt gatggacttc aacatgccgg
    15721 agctcggcgg gctcgatgtg gtgcgggcct atcggttcat cgatccggag gcggccgagg
    15781 ttcccgtgat catgttgtcc gcggacgtga ctccggacgc catgaaagag tgcagagagg
    15841 ccggcgtcga tacattcctg gcgaaaccca tagaagcacg acgcctgctg gacgagatcg
    15901 cggccctgct cgccaagcgc aatgccgcgc ggtcctcggc ctctgccaaa gtcgaacccg
    15961 gtgatgccgt cgtcctcaat acgaagacga tatccgacct ggagctaatc ggatcgggcg
    16021 gtcgcttcat gaccgaactc gtgaatggat tcctccagga cggcgaagcg ctgttgcggc
    16081 aaatggaaac cgcgatgaag gacgggaatt tcgaaaccct gcgggacatc atgcacgcaa
    16141 tgaagggatc cgccgccacg ctcggtactg atctcctctt ccgtacctgc tccgatatca
    16201 ccgcgctgac gaggtccgag ctgcaggcag ccgctcccca cgtgctgaga aacgtgcagg
    16261 accagttcca gcaggcgcgg atggctctgc tcgagtacct caagaaaagc caaaccctgg
    16321 cccgttaggc cggtgcgctg atttcgggcg aatcgccggc gaaaccgtct tacgcggtgg
    16381 cgagttccgg ctctctgacg ggctcgctca cgagaggctt cgcgccccgc ttgagctgcg
    16441 aacgtgcgag gcggctcatg agcttgagat ccttttcctc gagcctcagc gcggcatcca
    16501 cccagagcgt ggtgatcttg agcagctctt cgtacgaaat cgggttcacg tactggcggc
    16561 attcgaggac cgcttgcagc ccgttccgac ggcgctcgtt ctgcctgaca tagtcgtaga
    16621 ccgccgattc gccggtaccg tcttcgacca gcacgtcgac gacgcccatc tcgtgcagct
    16681 cggccgcggt gtagatcctc ccggacagga taatctcctc ggcgcgcctc atgcccacgc
    16741 gacgtgcgag caggctgtaa gcacccatgc ccgggaacaa gttgaacatg atctccggca
    16801 tgcccatcct tgcccgcttc tcggcaacga tgactccgga ggatagtgcg gtctcgaatc
    16861 ctccgcccaa ggcctcgccc tgcacgagcg agatcgtgat gatctggctg tgataggcgc
    16921 agatcctcgg aaacagcgcg tcgatgcaaa gcttggaata gtgcatcagc gtctcgcggt
    16981 cgccggaccg gatcagcggc aggaggatgt tgaggttccc cccgaggctg aagacgttct
    17041 cgctccgcga ggcgcccacg tagtaacgga tctgatgcaa ctcggttccg tgcagcaggc
    17101 ggccgccgct cgattggatg gctgcatcgt gagccctcag ctcctcgagc atctccgggt
    17161 tgaagcacgg agcgcccgca ggattgaggt aggtccacgc gattgcgtgc tctgggtcga
    17221 accttgtctc gatctgatgg tatttctttt cgaaaggcga gggggagagg ccgaagtcca
    17281 cgcgtgcgtt catgtgaatc ctcccggcaa gtgaagggaa tctggtaacc caagtgcggc
    17341 ttcaggggca atatacatca aacgatgccg ccggcataag ccatgcccat gtacggtgcg
    17401 gcgtcgcggc aggtcccagc acccggggtg tcgtgctggc agtgcgcccg cgcgtaggaa
    17461 gccgcggacg tacattgaag gattggctcc ccgaccaggg ctcgaacctg ggacctgcgg
    17521 attaacagtc cgtcgctcta ccagctgagc tatcggggaa acgtgatgct cggaccgatg
    17581 gcgtccgaga cattcgggaa ggcgcgtatt ttactgacgc cccacccggg ggtcaattcg
    17641 ggagcactga ctgcccaggc gaccgaatca aagcaccttc gcgatcgact cggcgacgta
    17701 gtcgatgttc cggctgttga ccgccgcgac gcagatacgg ccgctctcga tcgcgtacac
    17761 gccgaattcc tcgcgaagtc gaacgacttg ctcccggctc agccccgagt acgagaacat
    17821 gcctcgctgc ccgagaacga agccgaaatc ggctcgcggt gcgcgctgcc gcagccgttc
    17881 gaccagcgcg gcgcgcattt ccttgatccg gtcgcgcatt tgggtgagct cgcgctccca
    17941 cagcgcgcgc agctccggcg tcgtcagcac agtggcgacg acgcgacccc cgtgcgtcgg
    18001 cggattcgag taattcgtgc ggacgaggcg cttgagctgg ctcaacacgc ggttggcctc
    18061 gtcggccgtc ggtgccacca cgctcagcga gcccacgcgc tcgccataga gggacaggga
    18121 cttggaaaac gagctcgaga cgaacaccgg tatcccgaga tcggcaaagc ggcgcaccgc
    18181 cgaggcatcc tgctcgatgc cgtccccgaa accctggtac gcgaaatcga ggaagggcag
    18241 cagacggcga gcgacgaccg cgtcgatgac ggcatcccat tgctcgccgg acagatcgac
    18301 cccggtgggg ttgtggcagc aggcgtgcag gacaaccacc gtgcccgcag gcagcgactt
    18361 cagggtggtc atcatcgcgg caaagtccac cccgtgggtc ttcgcgtcgt aataagggta
    18421 ggcgcggacg gtgaatccgg cgttctcgaa aaggccgcgg tggttctccc aggtgggatc
    18481 gctgatccag gcttcggccc ccggggcgag ccgcctcagg aaatcggctc cgaccttcaa
    18541 tccgccggta ccgccgagcg cctggacagt gacgatccgc ccttccttga gggcgcggga
    18601 gtccgccccg aacaccagcc gctgcaccgt ccggtcgtac gcgggcaggc cgtcgatcgg
    18661 caggtagccg cgcggcgcgg cctgctcgac gagcatccgc tcggcgcggc gcacgcattc
    18721 gagcaggggc accttgccct cgtcgtcgta atacacgcca acgccgaggt tcactttcct
    18781 ggggttctta tcggcgagga acgcctccgt cagcccgagg atcggatcgc gcggggccag
    18841 ctcgaccgag gccagggggg agggggaatg gggagcgttc atcgtggtaa actcgattgt
    18901 ttgcgctgca ccattgtacc cgctgcgtct gtgatcgtca ctttcccgaa cagtcctttc
    18961 cagctcgagc aggcttacgt tccggcgggc gatcagcccg aggcgatcga caagctggcc
    19021 gacggtgtcc gggacggtct ttcctaccag accctgctcg gagtgaccgg atccggcaag
    19081 acctacacga tggccaacgt catcgcgcgg ctggggcggc ccgcgctcgt gatcgcgccg
    19141 aacaagacgc tggcggcgca gctgtactcg gagttccggc aattcttccc cgggaacgcg
    19201 gtggagtact tcgtctcgta ttacgactac taccagcccg aagcctacgt gccgtcgcgc
    19261 gacctgtaca tcgagaagga ctcgagcatc aacgatcaca tcgagcagat gcggctctcg
    19321 gcgaccaagt cgctgctcga gcgcagggac acggtaatcg ttgcgacggt ctcctgcatc
    19381 tacggtatcg gcgaccctgt cgactaccat ggcatgatcc tgcatttgcg ggaccgcgag
    19441 cgctacgcgc agcgcgacat catcgcgcgc ctcgccgcca tgcagtacga gcgcaacgag
    19501 ctcgatttcc gacgcggcac gttccgcgtg cgcggcgacg tgctcgacgt ctttcccgcc
    19561 gagcactcgg agaccgcgct gcgaatcgcc ctcgccgacg acgagatcga cagcctgacg
    19621 ctgttcgatc cactcacagg ccagacgcat caccgggtgc cgcgcttcac cgtgtatcca
    19681 tccagccatt acgtcacgcc gcgcgccacg acgctgcgcg ccgtcgaggc gatcaaggtt
    19741 gagctggcgc agcgcatcga ccatttccac aaggccggca agctgctcga ggcgcagcgg
    19801 atcgagcagc ggacgcgatt cgacctcgag atgctggccg agctcggatt ctgcaaaggc
    19861 atcgaaaact attcgcgcca tctgtccggc aggaacgaag gcgagccgcc tccgacgctg
    19921 atcgattacc tgccgcagaa ctcgctgatg ttcatcgacg agtcgcacgt caccctcggt
    19981 cagctgggcg gcatgtaccg cggcgaccgc tcgcgcaagg agaacctcgt cgactacggg
    20041 ttccgcttgc cgtcggctct cgacaatcgg ccgctgcgct tcgaggagtt cgagcgcatg
    20101 atgccgcaga cgatcttcgt ctcggcgacg ccggccgact acgagcgcga gcgctcgggg
    20161 caggtggtcg agcagctggt taggcctacg ggcctcatcg atccgcagct atccatacgt
    20221 cccgcctcga cgcaggtgga cgacctgctt tcggagattc atctgcgcgt ggcgcggcag
    20281 gagcgggtgc tggtcacgac cctgacgaaa cgcatggccg aggagctgac ggattacctg
    20341 gcggagcacg gcgtgaaggt gcgctacctc cattccgagg tcgagacggt cgagcgggtg
    20401 gagatcatcc gcgaccttcg cctcggggaa ttcgacgtgc tcgtggggat caatctcctg
    20461 cgcgaaggcc tcgacctccc ggaggtctcg ctggtggcaa tcctggacgc ggacaaggag
    20521 ggcttcctgc gctcggagcg ttctctgatc cagaccatcg gccgggcggc acgacacatc
    20581 aacggcatgg cgatcctcta cgccgacagg gtcaccgatt ccatgaagcg ggccatcggg
    20641 gagaccgacc gccggcgcgc gaagcaggtg tcgttcaacg agaagcacgg catcacgccc
    20701 attggcatca ccaagcaggt tcgcgacatg atcgagggcg tctacgatgc gcgcgaggtc
    20761 gagggcgaac tgaaggcggc gcaggcccag gcgcgctacg agtcgatgtc ggagaagcag
    20821 atcgcgaagg agattcgcca gctcgagaag cgcatgctcg agttcgcgcg aaatctcgag
    20881 ttcgagaagg cggcgcaggc gcgcgatcag ctcgcggagc tcaagaagag ctttttcgga
    20941 gcgtctcccg aggacgaaga cgagcccggc cgccgggctg gagcgagcgg ctgatgagcg
    21001 gagcttcgga tcgcccggtg cgcgtgcttt tcgtctgcac cggcaatatc tgccgctcgc
    21061 cgacggccga aggcatcttt ctgaagctgg tccacgacgc gggactggac caggagattc
    21121 gagtcgactc cgccggcacg cacggatacc acgtcggcga gccacccgac ctgcgcacgt
    21181 gcgaggcagc cgcgcaccgc ggctacgatc taagcgccct gcgggcgcgc aagttcgagc
    21241 gcgccgattt ccacagcttc gacctggtgc tggcgatgga ccgcgagaac cactcccatc
    21301 tcgtgcggct cgtgcctccg agcgaaggcc acaagctgaa gatgatgatg gagtactcga
    21361 ggcgcttcag cgggcgcgag gtgcccgacc cgtattacgg cggctcgcga ggcttcgagg
    21421 tggtcctcga catgctcgag gacgcctcgc gcgggctgct cgaagctatc gtcgaaaagc
    21481 aaagggcgcc cggaccgtaa gccggacgcc cttcgcgcga ccgattagct gggcgcgtcc
    21541 tctttacgcg tctcgacctg cacgagaggc tggttgagct ccgcaggcgg gggcctgcgc
    21601 tcgcgtcgcg cgggaacgaa ctccgactcg accggcactt cggcctgcac gccgggcttg
    21661 gtctgaacca gctctaggcc gcttctctcg agcgcgcgtt cgatttcggc cggatcctgc
    21721 gcgggggcgg gtggctcggg cgcgcgctcg cgcacgaccg gccgggcctc gatctcatgg
    21781 acgacgggct ggtcctcggc ctcccgcgcg accggcgggg cctcgggctc gtgaacggac
    21841 ctcggcgcct ggcgggcggg ctggtgcgcg tcgacttcgg agtcgcttgc ggctgccaac
    21901 tcggcggcgt gaaccgcccg ctggaaatcg cggatcgcct gcgatccagc cgcggccgtc
    21961 gccggatcga ccgtagggtt gccggcctga tccgggcggc gctcgctccg cgccggctgc
    22021 tccctgcgct caccgcggtc gccgcgatcg cgtccgccac ggcggcgacg gcgtccgccg
    22081 cgctcgccgc gctcgcgttc accgtcgtga ccatgcccgg ggccctgtgg ggcagcctgg
    22141 ccttggcgct cggcctgagg cggacgcgcc tgctccgctt gaggctgctc gccgcgctcg
    22201 agccgctgct gcggctcccg cggcgggcga ttctcgcctt ggggccgatc ggagcgttgc
    22261 tgccggtctc tttcctgacg aggcggacga tccggtcgac cttcgcgccg ctcttcgcgc
    22321 ccgggccgct gctcgggccg gccttcgccg cggttgcgga agcggtcgct atcgccgcga
    22381 tcacggcgat cgcgccggtc gcgattgtgc tcgggcctgg gttcacgctg cgcggctgac
    22441 tgcaccggcc gcggctccga caccaccgcg gaggccgcgg gctgctcgcg tcggaaccag
    22501 ccgaaaatgc gatcgatgat ccccgacccg gcttcgccga gggccggtcc ggagcgggcc
    22561 tgtggctgcg ggacgggctg gggccgcgca gccggcacgg gcgccggctg ctccggcgtg
    22621 acgccctgga cgacggcctg ctgccgcggg gcgcgctgct ccggggcggc cgcggagctc
    22681 agctcctcgt cggccggcac actcacgagg ttgtagctca cgggtatcgc gtcggcctgg
    22741 ttgaggtcgt cgtggcgcag ccgctcgacg tggtagttcg gcgtctgcag gtgcacgttc
    22801 gggatcagca cgacgttcac cttcaggcgc gcttcgatcg aatgaatgtc gctgcgcttc
    22861 tcgttcagca ggaacgtcgc gacatcgacc ggcacctgtg cgtgcaccga ggcgctgttc
    22921 tccttcattg cctcctcctg gatgatgcgc aggatatgga gcgcggtgga ttcggttccg
    22981 cggatgtggc cggtgccgtt gcagcgtggg caggtgatgt tgctgccttc gccgagcgac
    23041 gggcgcaggc gctggcgcga cagctccatc agcccgaagc gcgagatctt ccccatctgc
    23101 acgcgcgcgc gatcgaagcg cagcgcgtcg cgcagcttgt tctcgacatc gcgctggttg
    23161 cgctggttct ccatgtcgat gaagtcgatg acgatcaggc cgcccaggtc gcgcagccgc
    23221 agctgccggg cgatctcttc cgctgcttcg acgttggtct ggaacgccgt ctgctcgatg
    23281 tcgtggccct tggtcgcgcg cgccgagttg acgtcgatcg agaccagcgc ctcggtatgg
    23341 tcgatcacga ctgcgccgcc cgatggcaag ctgacctgcc gcgagtaggc cgtctctatc
    23401 tggtgctcga tctggaagcg cgagaagagc ggcacgtcgt cgcggtagcg cttcacgcgg
    23461 ttgacgttgt cgggcatgac gttgcccatg aaggcgagcg tctgctcgtg gatcgcgtcc
    23521 gtgtcgatca ggatctcgcc gatgtcgggc tggaagtagt cgcggatggc ccggatgacg
    23581 aggctgcctt cctgatagat caggaaaggc gccgggttga ggagcttccc gtcgccgtcc
    23641 ttcatcggct cggtcgcctc gacgatcttg tcccagagct tgaggaggta gtcgaggtcc
    23701 cacctcagtt cctcgagcgt gcggccgatg ccggccgtcc tgccgatgat gctcatgcct
    23761 tgcggcacct gaagctggtc catcaggtcg cgcagctcgt tgcggtcctc gccctcgacg
    23821 cgtcgcgaca cgccgccgcc gcgcgggttg ttcggcatca gcacgagata gcggccggcg
    23881 aggctgatga aggtggtgag cgcggcgccc ttggtgccac gctcgtcctt ctcgacctgg
    23941 acgatcagct cctggccttc cgcgatcgcg tcctggatgc gggcgcgatt gacgtcgatg
    24001 ccgtccttga agtactgccg cgcgacttcc ttgaatggca ggaagccgtg ccggtcggtg
    24061 ccgtaatcga cgaacgcggc ctcgaggctc ggctcgacgc gggtgatgac acccttgtag
    24121 atgttgcttt ttctctgttc cttcgcggcc gattcgatgt cgaggtctat cagcttctgg
    24181 ccatcgacaa tcgcgacacg gagctcctcg gcctgtgtcg cattgaacaa catacgcttc
    24241 attgtcttgt tctcccgcgc acacgcgggc aggacaagcc acgagcctaa atattcaggc
    24301 cggcttcaca tatgaattgt gtgggcacgt aggtgggctt gtcggtcggg tcgctgacgg
    24361 cgcgctgcgg ccgaacaggg accggaaact ctgccgatgt tatgggcgcg ctaaactatt
    24421 gctaactggt tggatgacca accgctattt gccggcttcg ggtttgcacg cttgctcgct
    24481 gggcgatgcc cggcgcagcg gtcgaaccgg ggacgaatgg acccgcctgg tcggacccgg
    24541 tcatgccggt ccgcggttca gacggccgtc ccgcaacgga agcagcatcc agatcataaa
    24601 gaaaatgaag gatttaagca atgcctccgc cgtcgcagtg gaagtcgacg cagggagcga
    24661 ggggcaacgc gtcgataatt ttctcctgcg agtctgcaaa ggcgtgccgc gcagccatat
    24721 ctatagggtg ctgcggacgg gcgaggtcag ggtgaatagc cggcgcgtcg gcgcaaccta
    24781 tcgtctccag ctcggggacc gcgtccgcat tcccccgatc cgggtcgcac ccgccacgcc
    24841 ccgacgcgcc ccgccggtcg aactgccgat cgtcttcgag gacgattccc tgctcgtggt
    24901 ggacaagccc tcgggcatcg ccgtgcacgg cggcagcggc gtcagcttcg gcgtgatcga
    24961 gcggctgcgc tcggcgcgcc cgcaggcgcg gttcctcgag ctcgcacacc ggctcgaccg
    25021 tgagacctcg ggactgctga tgctcgcgaa gcggcgcacg gcgctggtgg ctctgcacgc
    25081 ggcgctgcgc gacgggcgcg tgcgcaagcg ttacctcgtc ctcgtgaagg ggcggtggcg
    25141 cgacgccgag cgcagcgtcg accttccgct ccggaaatcg atcagcgcgt ccggcgagcg
    25201 gcgggtgagc gtggaccgct cgggccgcga ggcgctgacg gtgttccgcc gggtgcgcga
    25261 ggttccgggc tatacactgc tcgaagccga gctgaagacc ggccgcacgc atcagatccg
    25321 cgtgcatctc gcgcacctcg ggtttccgat cgcaggcgac gacaagtacg gcgatttcga
    25381 gctcaatcgc gccctggcga agcgaggtct caagcgcatg tttctgcacg cggccgagct
    25441 cgagctcgcg catccggtca ccggggcggc gctgagtctc acatcgcccc tgccgcgcga
    25501 gctgcacctg ttcctggact cggtccagcg ggaggtgcaa tgagcgcgag gcgctacgat
    25561 ctcctcgtgt tcgactggga cggcaccctg atcgactccg cgccgaccat cgtggcctgc
    25621 atccagtccg catgccgcga cctggggctg gccgtgcccg acgacgagcg cgcgagccat
    25681 gtcatcgggc tcggtttgcg cgatgccctg gcgcatgcgg ttccggggct cgacccggac
    25741 gagtacggcc gggtcgtcga acgatatcgg caccatttcc ttcagcggga tccgcacatg
    25801 gcgctgttcg acgggacgcg cgagatgctg cacggcctgc gcgacgcagg gcatgttctc
    25861 gcgatcgcca cgggcaagag ccgggcggga ctcgcgcgcg cgctcgagtc gacccgcctc
    25921 gggccgctct tctcggcgtc gcgctgcgcc gatca
//
LOCUS       DATDMW010000249         6289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_448874, whole genome shotgun sequence.
ACCESSION   DATDMW010000249 DATDMW010000000
VERSION     DATDMW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448874"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1051
                     /locus_tag="VI319_10760"
     CDS             77..1051
                     /locus_tag="VI319_10760"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864367.1"
                     /translation="MKLALRAAACGLAYLFAGGCLAQAQWPAKPVRLIAPFAPGGPVD
                     IIGRLVGAKLADSLGQQVLIDNHPGAGGNIGTVQVAKSAPDGYTGLVTSSAYVVNPSL
                     YKNAGYDPVRDLVAVAIAATQPNVIVANAAAFPSGSLEQVLAQAKAKRVSGFATPGSG
                     TTPHLTAEHVLRSLAHLDMQPIHFKGAGPAVTGVVSGEPPIGSMAISGPLPFIRSGKL
                     RALAVSSAKRVPALPDVPTLAEAGFPGVEDYTWVGMFLPGGTPDAIVQKLNEAVNRAI
                     RSPDVRARLDQLAFEPVGGSPREFADYVKAEVPKWRKVIQDAHIEPTE"
     gene            1097..2197
                     /locus_tag="VI319_10765"
     CDS             1097..2197
                     /locus_tag="VI319_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY6864368.1"
                     /translation="MTVDGLNQRLSRLHREVCEYFPFVVRISVALHDPQTDEIKTFLH
                     SPIDETPLAHYRVPLSQAGWLNNVRESRRSRVIGELEPATLGKQAHSLRILAAGYKAS
                     YTVPIFDENRFLGFVFFNSDKSGVFSDRVTRQLDLFVRIISLTIESALRFVDILVGSL
                     NLLREISGFRDVETSSHLSRMSYYSEIIAREAAPELGQPDTWVEYLRLFAPLHDIGKI
                     ATPDAILLKAGRLNDAEYEIMKQHAAKGEAIVTTLIDALALGSLPHVECLRNIARHHH
                     ERWDGSGYPDGLRGDQIPPEARIARIADVFDALTSRRCYKEAWPVETARKYVEERAGT
                     EFDPRFSEIFLSRNEKIAEIMGRFKEESSEAT"
     gene            complement(2207..2716)
                     /locus_tag="VI319_10770"
     CDS             complement(2207..2716)
                     /locus_tag="VI319_10770"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY6864369.1"
                     /translation="MKRDAGLSATDVTRAQVEDFLYREAALLDEWRLDDWLALLTADA
                     RYHVPSNDRPDADPASTLFTIADDISRIRARVTRLKDRNAHAEHPHSRTRRMITNVRI
                     AGREGDALRVEANFVVYRFRRDERIREYVGRYRYRLRVESGELKIAKREALIDAMELG
                     SLGSVSFIL"
     gene            complement(2713..3996)
                     /locus_tag="VI319_10775"
     CDS             complement(2713..3996)
                     /locus_tag="VI319_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6864370.1"
                     /translation="MNDRPLVRNDPAALDFQVNRATLVDPAILELERSRIFDVCWIYA
                     GHESEVRAPGDFRTRTVCGRPVIFCRDSRNAIRVFLNTCRHRGAMVCREPEGNARVYS
                     CFYHGWTYDRDGNLAGVPGEASYPKSFRKCDWGLTEAPRVESYRGFVFVNFDAGAMPL
                     EEYLAGAKEYIDLIVDQSPSGRMEIIHGTQEYDIRANWKLLVENSFDDYHLLTTHSTW
                     LDYMRGSGLQMKKPEPGHLLPSHGVGRALGNGHGVVDNENFRGRPVAKWIPLYGEAAK
                     PEIERIRAELVARLGEERARRVADTNRNLVVFPNLVLNDGSSVTVRTFHPLAADHMRV
                     TAFALGPVEETPAARAIRLDSFLTFYGPGGFATPDDIEALEMVQAGLAATCREVPWSV
                     MTRGMEKTGDQLNTDELHLRAFWQQWDKLMRVGGA"
     gene            4063..5037
                     /locus_tag="VI319_10780"
     CDS             4063..5037
                     /locus_tag="VI319_10780"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864371.1"
                     /translation="MSTISRAGACAVLLALAAAPAARAQETYPSRNVTLVVPYTPGTG
                     ADILARLLGPKLAERWKVAVITDNRAGAIGNIGTDHVAKSPADGYTLLCVATSFGTNP
                     PLHKKLPFDPVKDFAPVVLLATSAVSLTVNPDFPAKSLAEFLDVARKSPGKYYYASPG
                     SGGPQHLAMELLKQELKFDMVHVPYKGSGGAITDLLAGHVQAMVISLQTAAPYVHGGK
                     LRMLAVMSAQRSSAFPDVPTLKEQGLGDLEVETWYGMFAPAGTPGAVVARINADLNAL
                     LKEADVRAFLEKQGMNAEGGTPERFGGLVKSELARWSRVVKAAGIKAD"
     gene            5047..5769
                     /locus_tag="VI319_10785"
     CDS             5047..5769
                     /locus_tag="VI319_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY6864372.1"
                     /translation="MSLKEDIACAYRILAEHGVIDAYGHVSARSDRDPSRYIMARHLA
                     PELVTDDDLIEYDLDSNPIDDNGRESVRERFIHGEIYKARPDVKAVVHNHSPSVVPFS
                     ITGAKMRGLFHMAAFVGDGLPNFEIRKAQKGTDLLVKTPYLGKALAKTLGGAPAALMR
                     GHGSVTVGETLPRAVGRSIYLELSAQMQLQAMLLAGRGGKIVYLDKAEVKASVPHQEY
                     NRAWPLWRAKALARIRADKQGR"
     gene            5769..>6289
                     /locus_tag="VI319_10790"
     CDS             5769..>6289
                     /locus_tag="VI319_10790"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6864373.1"
                     /translation="MPRSVRRVVTGHDASGKAIVLRDGDAPNAKLRQATGLTSILVWV
                     TDETPADVSGGADRAEREIGVAPPPRGSIFRVVDFPPLGGTRVDNAAMLREMGLSHGA
                     GGARSALMHRTKSIDYAVVMAGEIDMLLDDSEVHLRAGDVLVQQGTIHAWENRGTEAC
                     RIAFVLIDAEAPA"
BASE COUNT         1008 a         2129 c         2191 g          961 t
ORIGIN      
        1 ggccatggtc tcctcggggt ggtgtgatgc gatcgagccc ggatgctacc attgcggcat
       61 cccggaggag ggacccatga agctcgcctt acgcgccgcc gcgtgcggcc tcgcatacct
      121 gttcgccggc ggctgcctcg cgcaggcgca gtggcccgcc aagccggtgc gcctcatcgc
      181 gccgttcgcg ccgggcgggc cggtcgacat catcggccgg ctggtcggtg ccaagctcgc
      241 cgacagcctc ggccagcaag ttcttattga caatcatccg ggcgccggcg gcaacatcgg
      301 cacggtgcag gtcgcgaaat ccgcgccgga cggctacacg gggctggtca cgtcgtcggc
      361 gtacgtcgtc aatcccagcc tgtacaagaa cgccggatac gacccggtgc gcgacctcgt
      421 cgcggtcgcc atcgcggcga cgcagccgaa cgtcatcgtc gccaacgctg ccgccttccc
      481 gtcgggaagc ctcgagcagg tcctcgcgca ggcgaaagcg aaacgcgtct cgggcttcgc
      541 cacgccgggg agcgggacga cgccgcacct caccgccgag catgtgctgc gctcgctcgc
      601 ccacctcgac atgcagccga tccacttcaa gggcgcgggc cccgcggtga cgggcgtcgt
      661 gtcgggtgag ccgccgatcg gatcgatggc gatctccggg ccgctgccgt tcatcaggtc
      721 gggcaagctg cgcgccctcg ccgtatcgag cgcgaagcgc gtgcccgcgt tgcccgacgt
      781 gccgacgctc gccgaagccg gcttccccgg cgtcgaggac tacacctggg tgggcatgtt
      841 cttgcccggc ggcacgcccg acgcgatcgt gcagaagctt aacgaggcgg tcaaccgcgc
      901 gatccgcagc ccggacgtgc gcgcgcggct cgatcagctc gcgttcgagc ccgtgggtgg
      961 atcgccgcgc gagttcgccg actacgtgaa ggctgaggtg ccgaagtggc gcaaggtcat
     1021 ccaggacgcg cacatcgagc cgacggagta agctcggggt tcggagagat cgccatgctg
     1081 cggaggaata tccggcatga ccgtcgacgg cctgaatcag cgactgagcc gattgcaccg
     1141 cgaggtctgc gagtatttcc cgttcgtggt gcgaatctcg gtggcgctgc atgacccgca
     1201 gaccgatgag atcaagacct tcctgcacag tccgatcgat gaaaccccgc tcgcgcatta
     1261 ccgcgttccg ctgtctcagg cggggtggct caacaacgtc cgcgagtcgc gccgctcgcg
     1321 tgtgatcggc gagctcgagc cggccacgct cggcaagcag gcgcattcgc tgcgcattct
     1381 cgccgccggc tacaaggcta gctacactgt cccgatcttc gacgagaaca ggttcctggg
     1441 cttcgtcttt ttcaactcgg acaagagcgg cgttttctcg gaccgcgtga cccgccagct
     1501 ggacctcttc gtcaggatca tatcgctcac gatcgagagc gcactgcgct tcgtcgatat
     1561 cctggtcggc agcctcaacc tgctgcgcga gatctcgggc ttccgcgacg tggaaacgag
     1621 cagccacctg agccggatgt cctactattc cgagatcatt gcccgtgagg cggcccccga
     1681 gctcggtcaa ccggacacgt gggtggagta cctgcgcctg tttgcgcctt tgcacgatat
     1741 cggcaagatc gcgaccccgg atgcgatact gctcaaggcc gggagactga acgacgccga
     1801 gtacgaaatc atgaaacagc acgcggcgaa gggagaggcg atcgtgacca cgctgatcga
     1861 cgccttggcg ctcggctccc tgccgcacgt cgaatgcctg aggaacatcg cccgtcatca
     1921 tcacgaacgc tgggacggca gcggatatcc ggacggactg cgcggggacc agatcccgcc
     1981 ggaggcgcgg atcgcccgga tcgcggacgt gttcgacgcc ctgaccagca ggcgctgcta
     2041 taaggaggcg tggccggtcg agacggcaag gaagtacgtg gaggaaaggg ccggcacgga
     2101 attcgacccg cggttttccg agatctttct ctcccggaac gagaagatcg ccgagatcat
     2161 gggccgcttc aaggaagagt cttccgaggc gacctgagcg cccgtttcag aggatgaagc
     2221 tgaccgagcc gagcgagccg agctccatcg cgtcgatcag cgcctcgcgc ttcgcgatct
     2281 tcagctcgcc cgactcgacg cgaagccggt agcggtagcg gccgacgtac tcgcggatgc
     2341 gctcgtcacg ccggaagcgg tagaccacga agttcgcctc cacgcgcaac gcgtcgcctt
     2401 cgcgcccggc gatgcgcacg ttcgtgatca tgcgccgcgt gcgcgagtgc gggtgctcgg
     2461 cgtgcgcgtt gcggtccttc agtcgcgtga cgcgcgcgcg gatgcggctg atgtcgtcgg
     2521 cgatggtgaa gagcgtcgaa gccggatcgg cgtcggggcg gtcgttcgac gggacgtggt
     2581 agcgcgcgtc cgcggtgagc agcgcgagcc agtcgtccaa gcgccactcg tcgaggagtg
     2641 ccgcctcgcg gtagaggaag tcctcgacct gcgcccgggt cacgtccgtg gccgagagcc
     2701 ccgcgtcgcg cttcatgcgc cgcccacgcg catgagcttg tcccactgct gccagaacgc
     2761 gcgcaggtgc agctcgtcgg tgttgagctg atcgcccgtc ttctccatgc cgcgcgtcat
     2821 caccgaccac ggcacctcgc ggcacgtggc ggcgaggccc gcctgcacca tctcgagcgc
     2881 ttcgatgtcg tcgggcgtgg cgaagccgcc ggggccgtag aacgtgagga agctgtcgag
     2941 ccggatcgcg cgcgcggcgg gcgtctcttc gaccggcccg agcgcgaacg cggtcacgcg
     3001 catgtggtcc gcggcgagcg ggtggaaggt gcgcacggtc accgacgagc cgtcgttcag
     3061 caccaggttc gggaagacga cgaggttgcg gttggtgtcg gcgacccgcc gcgcgcgctc
     3121 ctcccccagg cgcgcgacca gctcggcgcg gatgcgctcg atctcgggct tcgccgcctc
     3181 gccgtagagc gggatccatt tcgcgaccgg ccgcccgcgg aagttctcgt tgtcgacgac
     3241 gccgtggccg ttgccgagcg cgcggccgac gccgtgcgag ggcagcaggt gcccgggctc
     3301 gggcttcttc atctgcaggc ccgagccgcg catgtagtcg agccaggtcg agtgcgtcgt
     3361 cagaaggtgg tagtcgtcga agctgttctc gacgagcagc ttccagttcg cgcgaatgtc
     3421 gtactcctgc gtgccgtgga tgatctccat gcggcccgag ggggactggt cgacgatgag
     3481 gtcgatgtac tccttcgcgc cggcgagata ctcctcgagc ggcatcgcgc cggcgtcgaa
     3541 attgacgaac acgaagccgc ggtagctttc gacgcgcggc gcctcggtga gtccccagtc
     3601 gcacttgcgg aagctcttcg ggtacgacgc ctcgccgggg acgccggcga gattgccgtc
     3661 gcggtcgtag gtccagccgt ggtagaagca cgagtagacc cgggcgttgc cctcgggctc
     3721 gcggcacacc atcgcgccgc ggtggcggca ggtgttgagg aagacgcgga tcgcgttcct
     3781 gctgtcgcgg cagaagatca ccggacgccc gcatacggtg cgcgtcctga agtcgcccgg
     3841 cgcgcgcacc tcggactcgt ggcccgcgta gatccagcac acgtcgaaga tgcggctgcg
     3901 ctcgagctcg aggatcgcgg gatcgaccag ggtcgcgcga ttgacctgga agtcgagcgc
     3961 ggcgggatcg ttgcggacga ggggacggtc gttcatggct gcgaatgcgc ctcgaaggcc
     4021 tcattgtatg caagaatgcg gcccgaggag gaaagcggaa gcatgtccac gatctcgcgc
     4081 gccggcgcct gcgccgtgct gctcgcgctc gccgcagccc cggcggcgcg cgcacaggaa
     4141 acctacccgt cccgcaacgt cacgctggtc gtcccgtaca cgcccggcac cggcgccgac
     4201 atcctggccc ggctgctcgg gccgaagctc gccgagcgct ggaaggtggc cgtcatcacc
     4261 gacaaccgcg ccggggccat cggcaacatc ggcaccgacc acgtcgcgaa atcgcccgcg
     4321 gacggctaca cgctgctgtg cgtggcgacg tcgttcggca cgaatccgcc gctgcacaag
     4381 aagctgccct tcgacccggt gaaggacttc gcgccggtcg tgctgctcgc gaccagcgcg
     4441 gtgtcgctga cggtcaatcc ggatttcccg gcgaagtccc tcgccgagtt cctggacgtc
     4501 gcgcgcaagt ctccgggcaa gtactactac gcctcgcccg gcagcggcgg cccgcagcac
     4561 ctcgccatgg agctgctcaa gcaggagctc aagttcgaca tggtgcacgt gccgtacaag
     4621 ggctcgggcg gcgcgatcac cgacctgctc gccgggcacg tgcaggcgat ggtgatctcg
     4681 ctgcagaccg cggcgcccta cgtgcacggc ggcaagctgc gcatgctcgc ggtgatgagc
     4741 gcccagcgct cgagcgcatt cccggacgtg ccgacgctga aggagcaggg gctgggggac
     4801 ctcgaggtcg agacctggta cgggatgttc gccccggccg ggacgccggg cgccgtcgtg
     4861 gccaggatca acgccgacct caacgcgctg ctgaaggagg ccgatgtgcg cgccttcctc
     4921 gagaagcagg ggatgaacgc cgagggcggg acgcccgagc gcttcggtgg cctggtgaaa
     4981 agcgagctcg cgcgctggtc gcgcgtggtg aaggccgccg gcatcaaggc cgactgaagg
     5041 gacacgatga gcctcaagga agacatcgcc tgcgcttacc gcatcctcgc cgagcacggc
     5101 gtgatcgacg cctacggcca cgtcagcgcg cgctccgacc gcgatccgag ccgctacatc
     5161 atggcgcgcc acctcgcgcc cgagctcgtg accgacgacg atctcatcga gtacgacctc
     5221 gacagcaacc cgatcgacga caacgggcgc gagtcggtcc gcgagcgctt catccacggc
     5281 gagatctaca aggcgcgccc cgacgtgaag gccgtggtgc acaaccattc gccctcggtg
     5341 gttcccttca gcatcacggg agcgaagatg cgcgggctgt tccacatggc ggccttcgtc
     5401 ggcgacggtc tgccgaactt cgagattcgc aaggcgcaga agggcaccga cctgctggtg
     5461 aagacgcctt acctcgggaa ggcgctcgcg aagacgctcg gcggcgcgcc ggcggcgctg
     5521 atgcgcggcc acggctcggt caccgtcggc gagaccttgc cgcgcgccgt cgggcgcagc
     5581 atctatctcg agctgagcgc gcagatgcag ctccaggcga tgctgctcgc ggggcgcggc
     5641 ggcaagatcg tctatctcga caaggccgag gtgaaggcct cggtgccgca ccaggaatac
     5701 aaccgcgcct ggccgctgtg gcgcgccaag gcgctcgcgc ggatccgcgc cgacaagcag
     5761 ggccgctgat gccgcgctcg gtgcggcgcg tcgtgaccgg ccacgacgcg tcgggcaagg
     5821 cgatcgtgct gcgcgacggc gacgcgccga acgcgaagct gcggcaggcg accggcctca
     5881 cctcgatcct cgtgtgggtg accgacgaga cgcccgccga cgtgtcgggg ggcgccgacc
     5941 gcgccgagcg cgagatcggc gtcgcgccgc cgccgcgcgg gtcgatcttc cgcgtcgtcg
     6001 attttcctcc gctgggcggc acgcgcgtcg acaacgcggc gatgctgcgc gagatgggcc
     6061 tttcgcacgg cgcgggcggt gcgcgcagcg cgctcatgca ccggacgaag agcatcgact
     6121 acgccgtcgt gatggccggc gagatcgaca tgctgctcga cgactcggag gtgcatctca
     6181 gggccggcga tgtcctggtg cagcagggca cgatccacgc ctgggagaac cgcgggaccg
     6241 aggcctgccg gatcgcgttc gtgctgatcg acgccgaggc gccggcgca
//
LOCUS       DATDMW010000250        12344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1320097, whole genome shotgun sequence.
ACCESSION   DATDMW010000250 DATDMW010000000
VERSION     DATDMW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320097"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..2294)
                     /locus_tag="VI319_10795"
     CDS             complement(90..2294)
                     /locus_tag="VI319_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6864374.1"
                     /translation="MSDFEIVNLSRRDFLKAGAGLTLGVAAPGVLAQAAPGMAGGRPV
                     AEGIFEPNAFVRIGADNTVTVIVKHVEMGQGTFTGLPTLVAEELDADWRQVRAEGAPA
                     DASRYNNLFFGQFMGTGGSTALANSYEQMRKAGAAARAMLVAAAAERWGVSADAITVQ
                     RGVVAHAESKRRATFGQLAADAAKQPVPADVKLKDAKDFVYIGQRAPRTDAHAKSTGT
                     ATFTQDVKLPDMLVAVVAHPPRFGAKVKSFDASRAKDVRGVVDVLAIPQGVAVIASDF
                     WPAKKGRDALAVEWDESQAFRLGSTEIMAEYRRLAEKPGAPARWDGDPEGALAGAAKT
                     LEAAYEFPYLAHATMEPMNCVVRLSAEGCEIWNGEQFQSIDQPAVAGALDLKPEQVKL
                     NMLYAGGSFGRRANPHSDYVVEAASIAKALAQKRGGSATVKLVWTREDDMRAGSYRPM
                     YFHALKAGLDEKGNIVAWRQRIVGQSILAGTAFEAMMVKKGIDSTSVEGASTLPYRIP
                     TLGVELHSPKMGVPVQWWRSVGSTHTAFSTETFLDELAKAAGKDPFQLRRALLARAPR
                     HRAALELAASEGNWGQPLAEPARGDRRGRGIAVHESFSTVVAQVAEVTVLREGRVRVD
                     RVVCAVDCGVAVNPDIVRAQMESGIAYGLSAALHGAITLKDGMVEQSNFHDYVPLRIN
                     EMPRVGVYIVRSSEKPTGVGEPATPVIAPAVANAIFAATGKPVRSLPIKLEA"
     gene            complement(2351..2809)
                     /locus_tag="VI319_10800"
     CDS             complement(2351..2809)
                     /locus_tag="VI319_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6864375.1"
                     /translation="MIALNVNGKRVEVDADPDTPLLWVLRDRLDMTGTKYGCGMALCG
                     ACTVHLNGAPVRSCSMPVSAAVGKRIVTIEAIGATKEGRAVQAAWIANDVPQCGYCQS
                     GQIMSAVALLQKNKRPAEADIDAAMSGNVCRCGTYNRIRSAIRQAARELK"
     gene            complement(2835..4259)
                     /locus_tag="VI319_10805"
     CDS             complement(2835..4259)
                     /locus_tag="VI319_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6864376.1"
                     /translation="MAERGAISRRHFLGGLAALGAAGCVGPGARPAASRALPAAGDVL
                     IRDAHVMTMDPRLGDRPGARIHVSGGRIVAVGRDVEAPGAQVIDARGMIALPGLVETH
                     WHLWNTLLRSMSGDRRELGYFPTALALGKAYLPSDMYQGTRLGAAEAIHGGITFVHDW
                     CHNIRGPEFADQSLRALAESGVRARFSYGAAQGQPGDQPIDLADLERRARDWPRYANG
                     GLISLGLAWRGPSTGATGAVAASAYRQDFDAARRLGLPISVHANNRKGSDAIALLARE
                     RLLGRDVQVIHATWVSPEEIRALAESGASVSVSPYTEMRIGFGFPPTGEFLAAGIPLG
                     LSVDTTALSGNADMFAIMKAIQNIENGRAQNEFKLPARRVLELATIEGARSMGMADRI
                     GSLVPGKRADLILVDTRDVNLGVPSDPAHLLVEAAQPANVDTVIIDGRVLKRGGRLTA
                     VDVPAVVADASQANAALRKRAGWW"
     gene            4449..5669
                     /locus_tag="VI319_10810"
     CDS             4449..5669
                     /locus_tag="VI319_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6864377.1"
                     /translation="MTVAERPRHDTSIPDGDALFGSLFEQSAAGIGIVSLDGHFLRAN
                     PAYCRITGYTEAELQQKTFIDLTYPEDMEVTSSNRDALVSGEAKSRTYEKRYVHKNGT
                     PVWVQVVGSVVRDGAGRPTCTVAIVNDISGLKRTQAALESSEERFRRMVEMSSDWYWV
                     QDETFRFVELPGLAKRGLDAEVVMGKTRWEIPDLAPLPEKVWEQHRAKLLRHEPFTDF
                     IFLRYDKSGQTRYLSVTGEPIFDGAGRFKGYHGVGRDITGRARAQKALEDSEERYRTL
                     FEVHPNPMWVVDAKTLAFLAVNDEAVRQYGYTREEFLSMTADQIRPEEDVAELLKAFE
                     DRSRTYKQRVWRHKKKSGELMYVEIVSFNLEFDGKMARLGVINDITERVKMEERAQEI
                     EKKYQALLNERRQS"
     gene            5921..6250
                     /locus_tag="VI319_10815"
     CDS             5921..6250
                     /locus_tag="VI319_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864378.1"
                     /translation="MLNTKDWNHSPLLRRVEESIASEIRAIFEAFPDLAGFSVVDRSG
                     LPDDIDPSGRESQLFIGDIGFCPPVSDREHGRACNEICDALSDIVSERPEAFEVLKGR
                     TFSRILH"
     gene            complement(6341..7342)
                     /locus_tag="VI319_10820"
     CDS             complement(6341..7342)
                     /locus_tag="VI319_10820"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864379.1"
                     /translation="MTRNTLLRTLALAALIAAAGAVHAQSPSAGSGAAFPSRPVKIVV
                     PSSAGGATDTFARALAPQLSESWGQPVVIENRPGANQIIGSESVSKAAPDGTTLLVSE
                     ASSFVMNPHLYKKLPYDALHGFTPITALVRFPWVLVVHPSLPVHDFQELVAYARANPG
                     KLSYGSFGLGSSGHISMDYLKRLLGLDIVHVPYKGGGPAVADLVAGQISMMMVTPLLV
                     DGHARAGKLRLVAAVTAKRIERLPDLPTISESGVPGYEAGTWLAFMGPPGMAPETVAR
                     IYADTIKAMNDPAFREQHVTRQWFEVMGPPPAEFAGFLASEYERWGRLIRLSGVTVE"
     gene            complement(7384..8082)
                     /locus_tag="VI319_10825"
     CDS             complement(7384..8082)
                     /locus_tag="VI319_10825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY6864380.1"
                     /translation="MHDYRVREHVRQRVLKRKGRPLSLDAIDCARAALLVVDMQNYFV
                     APGFPQEVPVAREIVPAINRMAAAMRSAGGTVAWIQTTATGALQTWGNHHRYGMSPEV
                     SARRLATLAEDAEGYRLWHALEPSPVDLRVKKIKFSAMLPGSSDLHEVLTRRGVDTVL
                     VAGTTTNVCCESTARDAMMLDYRVAMLSDATAARSDEEHAASLDTFHVFFGDVMTVDE
                     ASARLVAVVRARTA"
     gene            8184..9182
                     /locus_tag="VI319_10830"
     CDS             8184..9182
                     /locus_tag="VI319_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6864381.1"
                     /translation="MRAPSRTRRALVSALLLAPGAALAQQPAPRKEAAAPAPRPKGPR
                     VFLDYDQKELDDAYDQSVYAPNLRQITGRYATNSEVTRTRLGPPRRLAYGPSEIEKLD
                     VYSPRRPGAPIMVFFHGGAWRSGRAADYAFPAETFVRSGAHFVAADFAPVQDVGGSLL
                     AMADQARRAVAWCYRNAASFGGDPNRLYVSGHSSGGHLAGVVAITDWVRDFGLPADVV
                     KGTVCISGMYELAPVRLSARSSYVKFTDEMVAALSTMRHVDRIKAPMVIAYGTFETPE
                     FQRQNREFAEALRAAGRPSLLVVAQGYNHFELPETLANPYGLVGRPALELMKIQPG"
     gene            complement(9199..10293)
                     /locus_tag="VI319_10835"
     CDS             complement(9199..10293)
                     /locus_tag="VI319_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY6864382.1"
                     /translation="MNKADLTLRAVRATGVEVPMKLPLGTSQGSIRAAPLLLIDVETE
                     QGVTGHAYLFCYVAAAAAGIAAMLAEVERVAKGERVSPADLWAKLARRFTLIGVQGIV
                     RMALAGFDVACWDALAKAAGKPLVSLLGGSPRTIRAYNSNGLGLMTPEKLADEAEALA
                     AGGFGAVKLRLGYPSVEADLAAVRAVRKRLPERVALPVDYNQALSVEEAARRGRALDG
                     EGVYWIEEPIRHDDYRGCARVARELSTPVQIGENFSLPHSMEEALAAGASDFVMPDLE
                     RIGGVTGWLRAAALAAGAGIEMSSHLFPEVSAHLLAVTPTCHWLEYVDWATPILEEPF
                     RIEDGHLVIPDRPGNGLAWNQDAVKRYRMT"
     gene            complement(10290..11216)
                     /locus_tag="VI319_10840"
     CDS             complement(10290..11216)
                     /locus_tag="VI319_10840"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY6864383.1"
                     /translation="MKIAMMGSGGVGGFFGGRLAHAGYDVSFVARGAHLAAMRERGLT
                     IENEPQGDIHVARVRATDDPGAIGPVDVVIVSVKLWDTEKAARQIKPLLGPRTGVLSL
                     QNGVVKDDILRRELGEAPVMGGVGYVATHISRPGVIHQVGTMQRIVLGEYDGRRSERA
                     EFLVEALKKAGVTAELSGDVRRAIWEKFVFLSALAGTTTAMRTTIGPIRQNPRTRAFL
                     LDLMREAVAVGRAHGVALPADYAEQRLAFADGLPADMTSSMHHDLERGNPLEVEWLSG
                     AIVRLGDAKGVPTPANRAVWDVLALHAAGRKA"
     gene            complement(11271..11861)
                     /locus_tag="VI319_10845"
     CDS             complement(11271..11861)
                     /locus_tag="VI319_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6864384.1"
                     /translation="MPGTRAAVFALACALAQPAAAEPGWYGFGSFARSSFGADGSSPL
                     AGGDDKDNGARVGAGYMFSESVGVEAGWTDFGKAKRGGAVPGLSPTRDGDVRANGPFL
                     AGVASVALSPRLSAFGKLGVIDARIDPSPDADAGIAGFFESSGDWRPMIGVGGTWRVT
                     RSWALQTEYGQFSKLIDRANEIKVSTLSVGMLLRLG"
     gene            11970..>12344
                     /locus_tag="VI319_10850"
     CDS             11970..>12344
                     /locus_tag="VI319_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY6864385.1"
                     /translation="MDASVACALAASALLGAGVVLANVGLRYVDSVRGALVAIPATTA
                     LYWVLAVFLLRADHFDPAALAIFAIVGVVFPAVATLLGYEANRLAGPTVTGTLSSTTP
                     LFAALGAVVFLGEALTPAVTAGT"
BASE COUNT         1822 a         4409 c         4299 g         1814 t
ORIGIN      
        1 gcaagcgacg atcagtgcac agggaatggt aagagcgaga gcgaacgcgc ggtggcgact
       61 tctcgaaagc tgccgcttca agattatccc taggcttcga gcttgatcgg cagcgagcgc
      121 accggcttcc ctgtcgcggc gaagatcgcg ttcgccactg ccggcgcgat caccggcgtc
      181 gccggctcgc cgacgccggt cggcttctcg ctcgagcgca cgatgtagac gccgacgcgc
      241 ggcatctcgt tgatgcgcag cggcacgtag tcgtggaagt tcgactgctc gaccatgccg
      301 tccttgagcg tgatcgcacc gtgcagcgcc gcgctgagcc cgtaggcgat gccgctctcc
      361 atctgcgcgc gcacgatatc cgggttgacg gcgacgccgc agtcgaccgc gcacacgacg
      421 cggtcgacgc gcacgcggcc ctcgcgcagc accgtgacct cggcgacctg cgcgaccacg
      481 gtgctgaacg actcgtgcac ggcgatgccg cgcccgcggc ggtcgccgcg cgccggctcc
      541 gcgagcggct ggccccagtt gccctcgctc gccgcgagct cgagcgcggc gcggtggcgc
      601 ggcgcgcgcg cgagcagcgc gcggcgcagc tggaacggat ccttgccggc cgccttggcg
      661 agctcgtcga ggaaggtctc ggtcgaaaac gccgtgtggg tcgagccgac cgagcgccac
      721 cactgcaccg gcacgcccat cttcggcgag tgcagctcga cgccgagagt cgggatgcgg
      781 tagggcaggg tgctcgcgcc ctcgaccgac gtcgaatcga tgcctttctt gaccatcatg
      841 gcttcgaacg ccgtgcccgc gaggatcgac tgcccgacga tgcgctggcg ccaggcgacg
      901 atgttgccct tctcgtcgag cccggctttc agcgcgtgga agtacatcgg gcggtacgag
      961 cccgcgcgca tgtcgtcctc gcgcgtccat acgagcttga ccgtggcgct gccgccgcgc
     1021 ttctgcgcga gcgccttcgc gatcgacgcc gcctcgacca cgtagtcgga gtgcgggttc
     1081 gcgcggcggc cgaagctgcc gccggcgtag agcatgttga gcttcacctg ctcgggcttg
     1141 aggtcgagcg cgcccgcgac cgcgggctgg tcgatcgact ggaactgctc gccgttccag
     1201 atctcgcagc cctcggccga gagccgaacc acgcagttca tcggctccat ggtcgcgtgc
     1261 gcgagatacg gaaactcgta cgcggcctcg agcgtcttcg ccgcgccggc cagcgcgccc
     1321 tcgggatcgc cgtcccagcg cgcgggcgcg cccggcttct cggcgagcct gcggtactcg
     1381 gccatgatct cggtggagcc gagcctgaac gcctgcgact cgtcccattc gacggcgagc
     1441 gcatcgcggc cttttttcgc gggccagaaa tcggacgcga tcaccgcgac gccctgcgga
     1501 atcgcgagga cgtccaccac gcccctcacg tctttcgcgc gcgaggcatc gaacgacttc
     1561 accttcgcgc cgaagcgcgg tggatgcgcg accacggcga cgagcatgtc gggcagcttc
     1621 acgtcctgcg tgaacgtcgc cgtacccgtc gacttggcgt gcgcgtcggt gcgcggcgcg
     1681 cgctgcccga tgtagacgaa gtccttcgcg tccttcagct tcacgtcggc cggcacgggc
     1741 tgcttcgccg cgtcggcggc gagctggccg aaggtcgcgc ggcgcttgct ctcggcgtgc
     1801 gcgacgacac cgcgctgcac cgtgatcgcg tccgcggaga cgccccagcg ctcggccgcg
     1861 gcggcgacga gcatggcgcg cgcggcggcg cccgccttgc gcatctgctc gtaggagttc
     1921 gcgagcgcgg tgctgccgcc cgtgcccatg aactgcccga agaacaaatt gttgtagcgg
     1981 ctcgcgtccg cgggcgcgcc ctcggcgcgc acctggcgcc agtcggcatc gagctcctcg
     2041 gcgacgaggg tgggcaaccc ggtgaacgtg ccctggccca tctcgacgtg cttgacgatc
     2101 acggtcaccg tgttgtccgc gccgatgcgc acgaacgcgt tcggctcgaa aatgccttcg
     2161 gcgacgggcc ggccgcccgc cattccgggc gcggcctgcg cgaggacgcc cggcgccgcg
     2221 acgccgagcg tgagcccggc tccggccttg agaaaatcgc ggcggctgag gttgacgatc
     2281 tcgaagtcgc tcatgacatc tccaaaagca gatccttcgc ttcgctcagg atgacgtgtc
     2341 atgcgacacg tcacttcagc tcgcgcgccg cctggcggat cgcgctgcgg atgcggttgt
     2401 aggtgccgca gcggcagacg ttcccgctca tcgcggcgtc gatgtcggcc tcggccgggc
     2461 gcttgttctt ctgcagcagg gcgaccgcgc tcatgatctg tcccgactgg cagtagccgc
     2521 attgcggcac gtcgttcgcg atccacgccg cctgcaccgc gcggccctcc ttggtcgcgc
     2581 cgatcgcctc gatggtgacg atgcgcttgc cgacggccgc gctcaccggc atcgagcacg
     2641 agcgcaccgg cgcgccgttg aggtgcacgg tgcacgcgcc gcacagcgcc atgccgcagc
     2701 cgtacttcgt gccggtcatg tcgagccggt cgcgcagcac ccagaggagc ggcgtatcgg
     2761 gatcggcgtc gacctcgacg cgcttgccgt tgacgttcag ggcgatcatg gaatgctcct
     2821 tgggaaaacg ggactcacca ccagccggcg cgcttgcgca gcgcggcgtt cgcctgcgag
     2881 gcgtcggcga cgacggcggg gacgtcgacc gcggtcagcc gaccgccgcg cttcaggacg
     2941 cggccgtcga tgatcacggt gtcgacgttc gcgggctgcg ccgcctcgac cagcaggtgc
     3001 gcgggatcgc tcggcacgcc gaggttcacg tcgcgcgtgt cgaccaggat gaggtcggcg
     3061 cgcttgcccg ggacgagcga gccgatgcga tcggccatgc ccatcgagcg cgcgccttcg
     3121 atcgtggcga gctccagcac ccggcgcgcg ggcagcttga actcgttctg cgcgcggccg
     3181 ttctcgatgt tctggatcgc cttcatgatc gcgaacatgt cggcgttgcc cgagagcgcg
     3241 gtcgtgtcga ccgacaggcc gagcggaatc ccggcggcga ggaactcgcc cgtcggcgga
     3301 aagccgaagc cgatgcgcat ctccgtgtac ggcgacacgc tcaccgacgc gcccgactcg
     3361 gcgagcgcgc ggatctcctc gggcgacacc caggtcgcgt ggatcacctg cacgtcccgg
     3421 ccgagcaggc gctcgcgcgc gagcagcgcg atcgcgtccg atcccttgcg gttgttggca
     3481 tgcaccgaga tcggcagccc gaggcggcgc gccgcgtcga agtcctggcg gtacgcgctc
     3541 gcggcgaccg cgccggtcgc gccggtcgac gggccgcgcc acgccagacc gagcgagatc
     3601 agcccgccgt tcgcgtaccg cggccagtcg cgcgcgcggc gctcgagatc ggcgaggtcg
     3661 atcggctggt cgcccggctg cccctgtgcc gcgccgtagg agaagcgcgc ccgcacgccc
     3721 gactcggcga gcgctctgag actctggtcg gcgaactccg gtccccggat gttgtggcac
     3781 cagtcgtgca cgaacgtgat cccgccgtgg atcgcctcgg ccgcgccgag ccgcgtgccc
     3841 tggtacatgt ccgaaggcag gtacgccttg ccgagcgcga gcgcggtcgg aaagtagccg
     3901 agctcgcgcc ggtcgcccga catgctgcgc aggagcgtgt tccacaggtg ccaatgcgtc
     3961 tcgaccagcc cggggagcgc gatcatcccg cgggcgtcga tgacctgggc gccgggcgcc
     4021 tcgacgtcgc ggcccaccgc gacgatgcgc ccgccgctca cgtgaatgcg cgcgcccggg
     4081 cgatcgccga gccggggatc catcgtcatg acgtgcgcgt cgcggatcag gacgtcgccc
     4141 gcggccggca gcgcacgcga agcggccggc cgcgcgcccg ggccgacgca ccccgcggcg
     4201 ccgagcgccg ccagaccacc cagaaaatgt cgcctgctga tcgcgccgcg ctcggccatg
     4261 gctcttctcg tcgtccgatg ggaagcccgc attataggcg cgccatgaca acgtcaaatt
     4321 tgtcctttgc cgcggggacg ggcggcctgt agattgctcg gcgacccgac gcgcgggtgg
     4381 cgtttcacac gcccgcgccg cactcgaata gaatcgggat ttgcgggaac gcggggggag
     4441 caggggccat gacagtggcg gagcgtccga ggcacgacac gtccattccg gacggtgacg
     4501 cgctgttcgg ctcgctgttc gagcaatccg ccgccggcat cggaatcgtc tcgctcgacg
     4561 gccatttcct ccgggcgaac ccggcctact gccgcatcac cggctatacc gaggccgagc
     4621 tccagcagaa gaccttcatc gacctgacgt atcccgagga catggaggtc acctcgagca
     4681 accgcgacgc gctcgtgagc ggcgaggcga aatcgcgcac ctacgagaag cgctacgtcc
     4741 acaagaacgg cacccccgtg tgggtgcagg tggtgggctc ggtcgtgcgc gacggcgcgg
     4801 gccggccgac gtgcacggtc gccatcgtga acgacatcag cggcctgaag cgcacccagg
     4861 ccgcgctcga gtcgagcgag gagcgtttcc gccgcatggt ggaaatgagc tccgactggt
     4921 actgggtgca ggacgagacc ttccgcttcg tcgagctgcc cgggctcgcc aagcgcggcc
     4981 tcgacgccga ggtcgtgatg ggcaagacgc gctgggagat ccccgatctc gcgccgctcc
     5041 ccgagaaggt gtgggagcag caccgcgcga agctgctgcg ccacgagccg ttcacggatt
     5101 tcatcttcct gcgctacgac aagtcggggc agacgcggta cctctcggtc accggcgagc
     5161 cgatcttcga cggcgccggc cgcttcaagg gctaccacgg ggtcgggcgc gacatcaccg
     5221 gccgggcacg ggcgcagaag gcgctcgagg acagcgagga gcgctaccgg acgctgttcg
     5281 aggtgcaccc gaacccgatg tgggtggtgg acgctaagac gctcgctttc ctcgcggtca
     5341 acgacgaggc ggtgcgtcag tacggctata cgcgcgagga gttcctctcg atgaccgccg
     5401 accagatccg ccccgaggaa gacgtggcgg agctgctcaa ggccttcgag gaccgcagcc
     5461 gcacctacaa gcagcgcgtc tggcgccaca agaagaagag cggcgagctg atgtacgtcg
     5521 agatcgtctc gttcaacctc gagttcgacg gcaagatggc gcggctcggg gtgatcaacg
     5581 acatcaccga gcgcgtgaag atggaggagc gcgcgcagga gatcgagaag aaataccagg
     5641 cgctgctcaa cgagcgccgc cagtcctgac gtcctccggg atgcgccgtt cggcgcatcc
     5701 cggccccgcc tcggacgatt cctaaccccc gtcggcgcct caggtttttc gaggtcccgc
     5761 cccggggagg gcccgattgg accgcctttg cgccgccttt aatctgcaat tgaccggcgc
     5821 gcattgccca cggacgccgg cgccgcagag agtcgaggcc agataaggga atggccggta
     5881 ccctgcggct gtcgcgggcg agcgaacgag gaaggcgacc atgctcaaca ccaaggattg
     5941 gaaccacagc ccgctgctac gccgggtcga ggagagcatc gccagcgaga tcagggccat
     6001 cttcgaggcg tttcccgatc tggccggctt ctcggtcgtc gaccgctcgg gccttcccga
     6061 cgacatcgac ccgtcgggcc gcgaaagcca gctgttcatc ggcgatatcg ggttctgccc
     6121 gcctgtcagt gaccgcgaac acggcagggc atgcaacgag atctgcgacg cgctctccga
     6181 tatcgtctcc gaacgtcccg aggcgttcga agtcctcaaa gggcgtacct tctcgaggat
     6241 tctgcactaa gcatcgcacg gccccttcag ggagagggcc cgcaaaacgg cgcgagaacc
     6301 gcgccgtttt gcatttgtgg cgccgcgatc ccgcggaggc ctattcgacc gtcacgcccg
     6361 agagccggat cagacggccc cagcgctcgt actccgacgc gaggaacccc gcgaactccg
     6421 cgggcggcgg acccatgacc tcgaaccact ggcgcgtcac gtgctgctcg cggaacgccg
     6481 ggtcgttcat cgcctttatc gtatcggcat agatgcgcgc caccgtctcg ggcgccatcc
     6541 cgggcgggcc catgaacgcg agccaggtgc ccgcctcgta gccgggcacc ccggattccg
     6601 agatcgtcgg caggtcgggc agccgctcga tgcgcttcgc cgtgaccgcc gccacgagcc
     6661 tcagctttcc ggcgcgagca tgcccgtcca cgaggagcgg cgtcaccatc atcatcgaga
     6721 tctgcccggc gaccaggtcc gcgaccgccg gcccgccgcc cttgtagggc acgtggacga
     6781 tgtccaggcc gagcaggcgc ttcaggtagt ccatgctgat gtgcccggac gagcccaggc
     6841 cgaacgatcc gtacgagagc tttccgggat tcgccctggc gtaggcaacg agctcctgga
     6901 agtcgtgcac gggcagcgag ggatgcacga ccagcaccca cgggaagcgc accagcgccg
     6961 tgatcggcgt gaacccgtgc agcgcgtcgt agggcagctt cttgtacagg tgcgggttca
     7021 tcacgaacga cgatgcctcg gacacgagca gcgtcgttcc atcgggcgcg gccttcgaca
     7081 ccgattcgga gccgatgatc tggttcgcgc cggggcggtt ctcgatcacg accggctggc
     7141 cccacgattc cgagagctgc ggcgcgagcg cgcgcgcgaa ggtgtccgtc gctccgccgg
     7201 ccgagctcgg cacgacgatc ttcacgggac gcgaaggaaa ggccgctccc gagcctgccg
     7261 aaggagactg cgcgtgaacg gcgccggcgg cggcgatgag cgccgcaagg gcgagcgtcc
     7321 ggagcagggt gtttcgagtc atgcgctcac gccggcatgc gagcttcggg gaaaggcccg
     7381 gtgctacgcg gtgcgcgcgc gcacgaccgc taccaggcgc gccgacgcct cgtcgaccgt
     7441 catcacatcg ccgaaaaaga cgtggaaggt gtcgaggctc gcggcgtgct cttcgtcgct
     7501 gcgcgccgcg gtggcgtccg acagcatcgc cacgcggtag tcgagcatca tggcgtcgcg
     7561 cgccgtcgat tcgcagcaca cgttggtcgt cgtgccggcg accagcaccg tgtccacgcc
     7621 gcggcgcgtc agcacctcgt gcagatcgga ggagccgggc agcatggcgc tgaacttgat
     7681 cttcttcacg cgcaggtcga cgggcgacgg ctcgagcgcg tgccagagcc ggtagccctc
     7741 ggcgtcctcg gcgagcgtcg cgaggcgccg cgcggacacc tcggggctca tgccgtagcg
     7801 gtgatggttg ccccaggtct ggagcgcccc ggtcgccgtg gtctggatcc atgcgaccgt
     7861 gccgccggcc gagcgcatcg ccgccgccat gcggttgatc gccgggacga tctcgcgcgc
     7921 gaccggaacc tcctgcggga atcccggcgc gacgaagtag ttctgcatgt cgacgacgag
     7981 gagcgcggcg cgcgcgcagt cgatcgcatc gagcgagagc ggccggccct tgcgcttgag
     8041 gacgcgctgg cggacgtgct cgcgaacccg gtagtcgtgc atgtcggctc cttgcggatg
     8101 agagcgaaga ataccgcagc cgcaccgttg ccgcctccgg cgctttcccg ttaatctgcg
     8161 cgctcattgc ggaggaatca atcatgcgtg cacccagccg cacccgtcgt gcgctcgtca
     8221 gcgccctgct gctcgccccg ggcgccgcgc tcgcgcagca gcccgcgccc cggaaggagg
     8281 cggccgcgcc tgcgccgcgc cccaagggcc cgcgcgtctt cctcgactac gaccagaaag
     8341 agctcgacga cgcgtacgac cagagtgtct acgcgccgaa cctgcggcag atcacgggac
     8401 gctacgcgac caacagcgag gtcacgcgca cccgcctcgg gccgccgcgc cggctggcct
     8461 atgggccgag cgagatcgag aagctcgacg tgtactcgcc caggcgcccg ggcgcgccca
     8521 tcatggtctt cttccacggc ggcgcctggc gctcgggccg cgcggcggac tacgccttcc
     8581 cggcggagac tttcgtgcgc tcgggcgcgc acttcgtcgc cgccgacttc gcgccggtgc
     8641 aggacgtggg cggcagcctc ctcgccatgg ccgaccaggc gcggcgcgcg gtcgcctggt
     8701 gctaccggaa cgcggcgagc ttcggcggcg atcccaaccg gctatacgtc tcgggccact
     8761 cctcgggcgg gcatcttgcc ggcgtcgtgg ccatcaccga ctgggtgcgc gatttcggcc
     8821 ttcccgccga cgtggtgaag ggcacggtct gcatcagcgg catgtacgag ctggcgccgg
     8881 tgcggctctc ggcgcgctcg agctacgtga agttcaccga cgagatggtg gccgcgctca
     8941 gcacaatgcg ccacgtcgac cgcatcaagg cgccgatggt gatcgcttac ggcacgttcg
     9001 agacgcccga gttccagcgc cagaaccgcg agttcgccga ggcgctgcgc gccgccggcc
     9061 ggccgagcct gctcgtcgtc gctcagggct acaaccactt cgagctgccc gagacgctcg
     9121 cgaatcctta cggcctcgtc gggcgcccgg cgctcgagct gatgaagatc cagccgggct
     9181 agagccggag gcgatcgttc aggtcatgcg gtagcgcttc accgcgtcct ggttccacgc
     9241 gagcccgttc ccgggacggt cggggatgac gagatggccg tcctcgatcc ggaacggctc
     9301 ctccaggatc ggcgtcgccc agtcgacgta ctcgagccag tggcaggtcg gcgtcaccgc
     9361 aagcaggtgc gcgctcacct cggggaagag atgcgacgac atctcgatgc ccgcccccgc
     9421 ggcgagcgcg gcggcgcgca gccagccggt gacgccgccg atgcgctcga ggtcgggcat
     9481 cacgaagtcc gaggcgcccg cggcgagcgc ctcctccatc gagtgcggca gcgagaagtt
     9541 ctcgccgatc tgcaccggcg tcgacagctc gcgcgcgacg cgcgcgcagc cccggtagtc
     9601 gtcgtgccgg atcggctcct cgatccagta cacgccctcg ccgtcgagcg cgcgcccgcg
     9661 gcgcgccgcc tcctcgaccg agagcgcctg gttgtagtcg accggcagcg cgacgcgctc
     9721 gggcaggcgc ttgcgcaccg cgcgcaccgc ggcgaggtcg gcctcgaccg acgggtagcc
     9781 gaggcgcagc ttcaccgcgc cgaacccgcc ggcggcgagc gcctcggcct cgtcggcgag
     9841 cttctcgggc gtcatgaggc ccaggccgtt gctgttgtac gcgcggatcg tccgcggcga
     9901 gccgccgagc agcgacacga gcggcttgcc cgcggccttc gcgagcgcgt cccagcaggc
     9961 gacgtcgaag cccgcgagcg ccatccggac gatgccctgc acgccgatca gcgtgaagcg
    10021 gcgcgcgagc ttcgcccaca ggtccgcggg cgagacgcgc tcgcctttcg cgacacgctc
    10081 gacctcggcg agcatcgccg cgatgccggc cgccgcggcg gcgacgtagc agaagaggta
    10141 ggcgtgcccg gtcacgccct gctcggtctc gacgtcgatc aggagcagcg gcgccgcgcg
    10201 gatcgatccc tggctggtgc cgagcggcag cttcatcggc acctcgacgc ccgtcgcgcg
    10261 caccgcgcgc agcgtgaggt cggccttgtt cacgccttgc ggcctgcggc gtgcagcgcg
    10321 aggacgtccc acaccgcgcg gttcgcgggc gtcggcacgc ctttcgcgtc gccgaggcgc
    10381 acgatcgcgc ccgagagcca ctcgacctcg agcgggttgc cgcgctcgag gtcgtgatgc
    10441 atcgacgagg tcatgtcggc gggcaggcca tcggcgaacg cgagccgctg ctcggcgtag
    10501 tccgcgggca gcgcgacgcc gtgcgcgcgc ccgacggcga cggcctcgcg catgagatcg
    10561 agcaggaacg cgcgcgtgcg cgggttctgc cggatggggc cgatggtcgt gcgcatcgcc
    10621 gtggtcgtgc ccgcgagcgc ggacaggaac acgaacttct cccagattgc gcggcgcacg
    10681 tcgcccgaga gctcggccgt gacgcccgct ttcttcagcg cctcgaccag gaactcggcg
    10741 cgctccgagc gccggccgtc gtactcgccg agcacgatgc gctgcatggt gccgacctgg
    10801 tggatcacgc ccgggcgcga gatgtgcgtc gcgacgtagc cgacgccgcc catcacgggc
    10861 gcctcgccca gctcgcggcg aaggatgtcg tccttcacca cgccgttctg cagcgagagc
    10921 acgccggtgc gcggcccgag gagcggcttg atctgccgcg ccgccttctc ggtgtcccag
    10981 agcttcaccg agacgatcac gacgtcgacc gggccgatcg ctcccgggtc gtcggtcgcg
    11041 cgcacccgcg cgacgtggat gtcgccctgc ggctcgttct cgatggtgag gccgcgctcg
    11101 cgcatcgcgg cgaggtgcgc gccgcgcgcg acgaagctca cgtcgtaccc ggcgtgcgcg
    11161 agccggcctc cgaaaaatcc gcccacgccc cccgagccca tcatcgcgat cttcatcgag
    11221 cttcctccga gcccgtatct taaggccgga agcgcaaagc cgggcgtgag tcagcccagg
    11281 cgcaagagca tgccgacgga gagggtgctg accttgatct cgttcgcgcg gtcgatgagc
    11341 ttgctgaact ggccgtactc ggtctgcagc gcccagctgc gcgtgacccg ccaggtgccg
    11401 ccgacgccga tcatcgggcg ccagtcgccg ctgctctcga agaagccggc gatgccggcg
    11461 tccgcgtcgg gcgaggggtc gatgcgcgcg tcgatcacgc cgagcttgcc gaatgcggac
    11521 aggcgcgggc tcagcgcgac gctcgcgacg ccggcgagga aaggtccgtt cgcgcgcacg
    11581 tcgccgtcgc gcgtcgggga caggcccggc accgcgccgc cgcgcttcgc cttgccgaaa
    11641 tcggtccacc cggcctcgac gccgacgctc tcgctgaaca tgtagccggc gccgacgcgc
    11701 gcgccattgt ccttgtcgtc gccgcccgcg agcggcgacg agccgtcggc gccgaagctg
    11761 gagcgcgcga acgagccgaa gccgtaccat ccgggctccg cggccgccgg ttgcgcgagc
    11821 gcgcaggcga gcgcgaagac ggctgctcgg gttcccggca cgatgctgac ccctcctcgc
    11881 ggtgaagcgc tacactgcga agaattacgc gcccgctttg tgacacaaca atgaagagaa
    11941 cagcgccgac cgaactgcgg cccggctgaa tggacgcgag cgtcgcctgc gcgctggccg
    12001 cgtccgcgct cctcggcgcg ggcgtcgtgc tcgccaacgt cggactgcgc tatgtcgact
    12061 cggtgcgcgg cgcgctggtc gcgatcccgg cgacgaccgc gctctactgg gtgctcgccg
    12121 tgttcctgct ccgcgcggat catttcgatc ccgcggcgct cgccatcttc gcgatcgtgg
    12181 gcgtggtctt cccggcggtc gcgacgctgc tcggctacga ggcgaaccgt ctggccggcc
    12241 cgacggtcac cggcacgctc tcgagcacga cgccgctgtt cgcggcgctc ggcgcggtcg
    12301 tgttcctcgg agaggcgctc acgcccgcgg tcacggccgg cacg
//
LOCUS       DATDMW010000251        36652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_946325, whole genome shotgun sequence.
ACCESSION   DATDMW010000251 DATDMW010000000
VERSION     DATDMW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 36652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946325"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1629
                     /locus_tag="VI319_10855"
     CDS             313..1629
                     /locus_tag="VI319_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864386.1"
                     /translation="MDRSPQQLARNVTRLSRLDLPGAGQVCVAGDSAYVGHIPNTKRL
                     GTSILDVSDPRSPRVVSQIFLDDPDTHSHKARVIGDVMIVNAERNMTAIGRKAEQLPL
                     ARAELRAALGREPTHEELAAKIGIAAADIPAVEHAERNPYGGGGFRVYDVSDRAKPRL
                     IGFQRTGGIGVHRFDMDTRYAYISTEMAGYVGNILVIYDIADPARPREVARWWMPGQH
                     LAGGETPAWVGKRNRLHHALRSGDRLWAGCWQAGLRVIDVSDIGAPRTIGEYNYHPPY
                     PEPSHTFMDVPFPVAGRRIALAIDEEDHAASRDEMERRRGRPHACLWVFDVSDVADMK
                     PLARYEVSELDSPWSRAAPGRFGAHQFREKMDGTLVYCAWFAGGLRVVDVADPLAPRE
                     VGFFIPEPAPGRVAPQTNDVDLDGRGLIYIVDRYAGLDVLELTPPR"
     gene            complement(1613..2347)
                     /locus_tag="VI319_10860"
     CDS             complement(1613..2347)
                     /locus_tag="VI319_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY6864387.1"
                     /translation="MKEWLAMCLVAGVAAASEAAEIKVISGGAIEPGLEAVAAAFEKQ
                     SGHKVHVTFNTAPQIRKRVAEGERFDVVIAPPAVLDELAKAGKVSGEGHVPAGRVGVG
                     ITVRSGAPAPAIGSVDELRQALLRADSVVYNQASSGLYLERLFDRMGIGEQLKPKTTR
                     YANGAQVLEHVIKGRGNEIGFGAMTEIRLFEPKGLKLVGPLPAEVQNYTSYSAVAAAS
                     SDVARAFVRYLGTPAAKRDFAANGVE"
     gene            complement(2363..3331)
                     /locus_tag="VI319_10865"
     CDS             complement(2363..3331)
                     /locus_tag="VI319_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864388.1"
                     /translation="MPRSRPLAVLVLALASAGALAQGAFPQKPVRFVVGFTPGGPSDI
                     LARALGQKLADTWRSQVVVENRPGAGGNVAAEAVARAPADGYTWLLGNNSILATNRSL
                     YRSLAYDPEKDFAPVALVAIQPNILVVNPQLPVRSVRELIALAKSKPGQLNYASSGSG
                     AAAHLAAELFKAMTGVDMVHVPYKGAQPALTDVIAGQAQLMFATSASVIPYIRAGRLR
                     ALAVTTARRSPSVPDLPTVAEAGVPGFEAITWHGVVVPAATPAALVERLNRDIVAALG
                     ERDLRERLESLGAEVASGTPSDFAQYIAAEIPKWSKVVKDSGARAD"
     gene            3408..4766
                     /locus_tag="VI319_10870"
     CDS             3408..4766
                     /locus_tag="VI319_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY6864389.1"
                     /translation="MRTLAAYIARAPRRALPAAVAEKTKHHLLDTLAAMISGSRLLPG
                     RQAIAFARTQGGAKEACIAGSRIVTSAVNAALANGMLAHADETDDSHAASLTHPGCGV
                     VPGALAMAERERAGGTALLRAVALGYDVCARLTLSLDPYQFREDGHSTHSFGPTFGAA
                     AAAGALVGLREPQVRHLLSYAAQQASGVSCWMRDERHVEKAFDFGGMPARNGVAAAAM
                     VAHGFTGVDDVFSGERSFFVAYGRKPDPARLAAGLGETWEIMNASIKRWSVGSPIQAP
                     LDALALLIAEHGVRAADIERVVVRVAHQGANTVNDRAMPDICLQHLCALMLIDRDVSF
                     RSSHDAKRMRDPKVLSLRARVELHGDDALSRAMPSRQGIVELRLRDGRTLRHHATEVR
                     GTPGNPMTRAEVHAKALDLIAPVTGRARAERLCDTVWNIERLRDVRALRPLLAHRQET
                     PA"
     gene            4763..5224
                     /locus_tag="VI319_10875"
     CDS             4763..5224
                     /locus_tag="VI319_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6864390.1"
                     /translation="MNRIVHIALKVDDLERSRQFYENIFGFRENKTEKVRDHTSCHLS
                     DGAIDLSIMQYDAGTQSAESKAAGEGPCIHHFAVEVNDMAAAERQIRAFGCEIISDPG
                     VVPVKFRAPGGTVCEIVPKLRYQKIRKAGPKAKAKSKAKAKAKARKGAKKK"
     gene            5249..5725
                     /locus_tag="VI319_10880"
     CDS             5249..5725
                     /locus_tag="VI319_10880"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY6864391.1"
                     /translation="MEETSEKSSDLWAGLALAALGVYIVVQAAQWEYIGPDGPGPGFF
                     PLWYGIAMVVLSAALVFVGVRARRARAAAASALFPWARIGRALLVWLAFAVSVALSEV
                     LGFTISFGLLCFFIVAVMYRRPLKVAAAVAVASALGFYTVFPLALGVSLPTGWVGF"
     gene            5727..7241
                     /locus_tag="VI319_10885"
     CDS             5727..7241
                     /locus_tag="VI319_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY6864392.1"
                     /translation="MEVLSGLAHGFGVALEPANLLWCFVGVFLGTVVGVLPGLGPPAT
                     VAMLLPLTFHMNPAGAIIMLAGIYYGAKYGGSTTSILMNVPGESASVITCIDGYQMAR
                     KGRAGAALGIAAIASFIAGTVGVVALMLVAPPVARFALSFSSPEYFGLMSLGLALVVL
                     LAGHSLLKALLAALLGLWIASIGTDLFSATSRFTFGRVELLSGVDFIVVAIGVFALGE
                     VFANMEKEDDAQLLPVPKGLRNLLPTMQDMKDCRFAFLNGSVVGFLVGALPGGGSTIA
                     SFLSYGLEKAVSRRREQFGTGVIEGVAAPEGANNSETGGALVPLLTLGIPGSGTTAIL
                     LAALVLWGFKPGPLFIPENPALFWGLVASMYIGNVMLLVLNLPLVPVFAQVLRTPTYI
                     LFPIILGVSLVGVYSVSGSMFDLLLLCFFGLLGYLMRKLDFPAAPLILGLALGAGMER
                     ALRQSLMMSGGQLSILVSRPISAAMLALALVVLLIPLVGKANAWRVRAIDQEQA"
     gene            7260..8231
                     /locus_tag="VI319_10890"
     CDS             7260..8231
                     /locus_tag="VI319_10890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864393.1"
                     /translation="MRKYWYYAIAAALACAATAHAQGYKPTRPVELVVHTGPGGGSDV
                     LARAVAVMFEREKLVPVRVNVVNKTGGNGNVAIAYLAEKKGDLNTIGFFTGAQLTSYL
                     VSKEAQVTIRDLTPVARLVLEPAVIAVKADSPYKTLRDFVDAAKKNPGQLKQSGGSIT
                     SRDNVVRELLQKASGARWAYISFPGGGERIAALLGGHVNMMVIEPEEAGEHIRAGNMR
                     VLAQVSEKRLARFPNVPTLKEAGFDVPVVPQVRGVVAPPGIPEANVRYWEDVFKRLTQ
                     TASFRKYVEENQFEMGYLDGAGLVKFMDSYFDQMRSILKDAGVKTVR"
     gene            8231..9601
                     /locus_tag="VI319_10895"
     CDS             8231..9601
                     /locus_tag="VI319_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY6864394.1"
                     /translation="MGVAADSVAERLAAGVAGLSASGIPRGVRETCAALLVDVGGLCV
                     AARASGYVRMLAAAVDRGGPCTAIGHGGGLAAEDAAMINGTAAHGEDFDDTFEGGPVH
                     SGAVVVPAVLAACERFGRGGDSALLGIAAGVETLCRLSLVAPKRIHKAGFHPTSVLGA
                     MAATAGVGAALGLDRRQTVDALGIAGSMAGGIIEYLAEGAWTKRLHAGWAARIGLHAA
                     RIGREGFLGPRTVFEGTHGLFFGFSHSAEGDYDALVRGFGRDWVMTGITFKPYATGTM
                     NQPYIDCALRLARKGFAAEDVAEVLCETAEGYVHRLWEPLAAKHRPPNDYAAKFSTPF
                     NIAVAFLTGGAGFEAFTERTVRDPRILALASKVRYVVDANNPYPKAYTGHVRVTLKDG
                     RVFEERQPHIRGGVQEPLTREDLEDKFRGNARHGGWEDALADRYLQFARTAFDAASLA
                     LGEFGA"
     gene            9782..10324
                     /gene="pal"
                     /locus_tag="VI319_10900"
     CDS             9782..10324
                     /gene="pal"
                     /locus_tag="VI319_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006409874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY6864395.1"
                     /translation="MKLQSQLSIAASLLALAFVAGCASEAKKDATSASPAASSPSSSG
                     AAPSAQSAQPSGVTGNALNDPKSMLFKRSVYYEFDQSNVKTDYRSLVEAHARYLREHP
                     GATITIEGNCDERGSREYNLALGQRRADGVKSMMTVLGVPDRQVDTVSYGEEKPKAQG
                     HSEDAWSQNRRSDIVYRKVQ"
     gene            10488..11771
                     /locus_tag="VI319_10905"
     CDS             10488..11771
                     /locus_tag="VI319_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6864396.1"
                     /translation="MALALVPAHRLARINYAPRALAFAYTFLVLEALMLERGSGGWML
                     ALGALQFLVYPHLAYLHARLVPESKRAELINLAFDALTLGAWTAQAQFALWWSAGLLS
                     AISLNSAANGGLRYLGLAWALFAAGAALWGGISGFSFSPDTGPLVTGLCIAGVIVYVS
                     WIGTIMRAQNRALMRTRGALHSSEDQFRFIAEHAGDLVAVLDPRGRIRYTSASHLEHF
                     EAALVAPGQDWVRLVHPDDRERARNFLQYLVMSRQMERMSLRLVPMDGPSLVVDCEGN
                     PAADDSDGMGMIILVMRDITTRVRTEMDLRLAAHAFDRVSDGVLISDGSGRIEYVNGA
                     YCTMTGYEPRDLVGRTTDEVRSALKGDELYHEIWRSVQRTGSWRGRFMEQRRDGKFFA
                     ASVVVSAVRDKEGASTHYVWVITDPAAPERAARSA"
     gene            11910..12614
                     /locus_tag="VI319_10910"
     CDS             11910..12614
                     /locus_tag="VI319_10910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6864397.1"
                     /translation="MAGTHIDIRAADGGKFRGYLCLPRSGKGPGLVVEQEIFGINENV
                     RLVCEHYANQGFVALAPDLFWRLQPNVDLGYSPEEWQKAFDFMKRFDIDKGIEDVGAT
                     IKHLRGMKECTGRVGAIGYCLGGKLAYLTAARTDVDCAVAYYGVGLDQNLNEKGKIGK
                     PLMMHIAEKDKFVPPEAQAKIKAGLKDMKNVEIHSYPDVDHAFARINGDHFDANAAEL
                     ANKRTLDFLKKHLMGR"
     gene            complement(12611..13645)
                     /locus_tag="VI319_10915"
     CDS             complement(12611..13645)
                     /locus_tag="VI319_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6864398.1"
                     /translation="MGAVRLAGLLVLAACLCGHALAQPAKRDYDAEIDAAIRSAKTAA
                     GFEFLGTLARTCLLPQSGGEDLSDKVPPYVADPAKAPPRETWYAEPSKVFDELYFVGG
                     KIHSSWALRTRAGLILIDTIYPYNSEELVLGGMEKLGLDPKDIKYLVISHAHADHIGG
                     AEMIQRRYHPRVVMGGPDWDLVERHPNRYKTMAPRRDIVATDGMKITLADTTLTVWLT
                     PGHTPGTLSYTFTVHDRGRPVNVAYSGGTAFNFVNDTPDPGIKNFQIYIDSQRHMAAK
                     AAETGATVLLSNHSEFDNAYNKNRMLAGRGKGPHPYEIGADWVQRYFQVMQGCARAAQ
                     IRLEKKAAER"
     gene            complement(13648..14187)
                     /locus_tag="VI319_10920"
     CDS             complement(13648..14187)
                     /locus_tag="VI319_10920"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY6864399.1"
                     /translation="MAKTAKRHLAKPAPRRRAAGSRDTQHAVEQFLYRQAELLDEKRW
                     DDWIDLFADDGVYWMPPEPSHATWEGMPAIFAEDKNLMTVRMKRVLHPDAWSQRPLWG
                     TNHVIGNVVLERELANGDVCVRSRFHMMELRRDDVRHFAGAYTHHLTRTRDGFRIKLQ
                     RVDMTNAQAAYDYVLQVWV"
     gene            complement(14187..15518)
                     /locus_tag="VI319_10925"
     CDS             complement(14187..15518)
                     /locus_tag="VI319_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6864400.1"
                     /translation="MLHEHVPNLGNYVEPDRVRREVYTDPAIFELEMERIHERVWIYC
                     GHESQVPRPGDYYTVQVGRQPMVMVRAGDGGVNVLYNRCPHRGNMMCGDRSGNAGEFF
                     RCSYHAWTFHHDGRLRSIPMQESGYAGTRWGKDNPDCSMKRAARVENYRGFVFASLAP
                     DGPTLAEFLGESRVAFDDMCDRAPEGEVEIVPNCFRVIQHSNWKIFLENQLDALHPSV
                     THQSTGRAAHEVEKIIAAKARKGEAPLSYHMLSAFATVTIDKWDGFQTLNYPNGHCIL
                     TGYMGLRPQDPDTLAYDRIMIEAYGKKRFEEIIGVNIHHVLIYPGLSVQSPLQQLRAV
                     RPLGVDKTLTEIWHFRLKGAPEAIYRRSLAYYNLVNSPATLVNADDLENFWKCHQGLA
                     SDGGDWVSFARHMGRDVERDGRVETAPNCGTSEAPMRNQMKAWARYMTAER"
     gene            complement(15635..15838)
                     /locus_tag="VI319_10930"
     CDS             complement(15635..15838)
                     /locus_tag="VI319_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864401.1"
                     /translation="MFMKHVQNYLMLTVAIAFIAVAIFPIVFFYGLSLLVEGFVGKKM
                     AAKIEAFWDAVGKPYNYLIQRVE"
     gene            complement(15910..16620)
                     /locus_tag="VI319_10935"
     CDS             complement(15910..16620)
                     /locus_tag="VI319_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864402.1"
                     /translation="MSAMLEIEGLETAYGASQVLFGVDLVVNAGEAATLLGRNGMGKT
                     TTVRSILGLTPARRGSIRFRGERIERLSPDRIARAGIALVPEGRQIFPNLSVRENLVA
                     FAANRCGSRDPWTLERVLDRFPGIAARLGHLGSQLSGGEQQMLAIGRALMTNPHLLIL
                     DEATEGLAPLVREEIWRCLASLKAGDQTILVIDKYVERLVELADHHTVIERGRVAWRG
                     SSRQLGGNPALWHRYLGV"
     gene            complement(16617..17378)
                     /locus_tag="VI319_10940"
     CDS             complement(16617..17378)
                     /locus_tag="VI319_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864403.1"
                     /translation="MTAPLLAAIELRKRFGGVPASDGVSLTVSPGEIHALIGPNGAGK
                     TTLIAQLAGALRPDSGRIELAGRDITALGPSGRVRLGLARSYQVTTLFGALGVLDNLA
                     LAVQARSGSSMSFWRPAAREKALYEQAAAIVARIGLAGRESVAAAHLAHGEQRTLEVG
                     LALATRPRLLLLDEPMAGMGPEESARMLALIQQLREAEPALSVLLIEHDLDAVFRLAD
                     RISVLVSGRVLATGTPAEIRADAQVRKAYLGEESA"
     gene            complement(17375..18352)
                     /locus_tag="VI319_10945"
     CDS             complement(17375..18352)
                     /locus_tag="VI319_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6864404.1"
                     /translation="MARLRPGAVVLGLVVAAALAFPFVMRAEGDAYYLSFASRILIYA
                     LAATSLNLVLGYGGMISFGHAAFVGTGAYVSSILLAEGVASAWIGWPAAIAASALAAL
                     AIGAVSLRTRGVYFIMITLAFAQMTYFLVNSMKAYGGDEGLTLPARARVGGGIDLDDA
                     QTFYYVALAALAAALVALHRLAHSRFGRVIRAIRENETRAEAIGFPVYRYKLVCFVIA
                     GAAAGLAGALLASHGKYVNPSVLHWTQSGSLMVMVIIGGVGSLWGGALGATALLGLED
                     LVANHRVAWVAALLPNYPQHAGLAVGLVLLAVVLFAPQGIAGLLARRPR"
     gene            complement(18354..19271)
                     /locus_tag="VI319_10950"
     CDS             complement(18354..19271)
                     /locus_tag="VI319_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6864405.1"
                     /translation="MSGILILEQALNGLQFGLMLFLLAAGLTLVFGIMDMINLAHGSL
                     YMLGAFLAATFVSLTGSFALGVLLAVPATAALGMLLEMSLLRRLYARDHLSQVLATFA
                     LILVFNEAVRIVWGTDLPLAAPEALSGPVELFPGMLYSSYRLLIIAVGAAAAGLLYYL
                     VAKTRIGMRVRAGASNREMALAMGADIPRLFTVVFGIAAGLCAVAGALLGPILAVQVG
                     MGENILIVAFVVIVIGGIGSVRGALVGSLIVGMVDTIGRAFLPIAFGAFLPPQLASAA
                     GPATASVLVYLLMAVILTLRPQGLFPARG"
     gene            complement(19273..20451)
                     /locus_tag="VI319_10955"
     CDS             complement(19273..20451)
                     /locus_tag="VI319_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864406.1"
                     /translation="MNKATVFCAAALAFAALPAAPARAADQVTVGFISTLSGPSAALG
                     IDIRDAFMLAVKMNGGKLGGLRADVIVGDDQFKPDVGRQLAEKNVKLDKVDILTGFVF
                     SNIMLASVPIAFENKVVYVSPNAAPSPLAGKQCNPWFFAASWPNDAYHEAAGQYATSQ
                     GYKAVYMLAPNYQAGKDSLAGFKRFYKGSIVGEVYTQLGQLDYSAELAQVRAAKPQAV
                     YIFLPGGMGINFIKQFVAAGLSRDIQLITPGFSSDQDIIRAVGEPMLGIHDAAHWALD
                     LDNAANRKFVAAFEKEYGRLPTVYASQGYDTALLIDAAVRDVKGKVEDREALRHALKA
                     AKFDSVRGPFKFNRNQYPIQNYYLRVVAKDAQGRLVNRTAGTIFKDRGDAYVDECPMK
                     "
     gene            20535..21260
                     /locus_tag="VI319_10960"
     CDS             20535..21260
                     /locus_tag="VI319_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6864407.1"
                     /translation="MGEARTRTAAGAALLLVALVAASAALAEDAPADSPAADWSGLYL
                     GAQFGGDTSDARWRTDATVADGDAVHQRASSPALGLQLGARFRVSRHWLVGAEGSYTL
                     ARLRTVAQSAELAERGLPGRSRETDLRNLYAVTVQAGYAHEDRWLLYGKAGVAGSYVY
                     LATANVLSGVVSSTNGRARGWTGGVGVEYLLADRWSFGLEYDHYELRLGDRSAIQSNG
                     AVADYTGFKGSIDALYARLNRTF"
     gene            21335..22540
                     /locus_tag="VI319_10965"
     CDS             21335..22540
                     /locus_tag="VI319_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6864408.1"
                     /translation="MEQQDLSRLRIDRAAAPLRRRRRGPWIAAAVAIVAVLAGFAYTR
                     FGAPSEVETVAVARIFPSQSLAVLNATGYVVAQRKAAVSSKATGRLEWLGVLEGSRVK
                     KGDIIARVESRDVAATVDQASANVGVAQANLEQGLAELADAESAFKRSEELLAQKYIS
                     PSAHDSAIARYHKARAAIVGLRAAIAAASANQRAAQVAFEQTLIRAPFDGVVLTKSAN
                     VGDIVTPFSAAVDTKGVVVTMADMETLEVEADVSEASLGKIRVDQACEIQLEAFPDVR
                     FPGVVSRMVPTVDRTKATLLVKVRFTQPDARVLPDMSAKVAFLSRAVTPEERSAVDAL
                     QRSAIVTRGGRSVVFVVRDGRVSEVPVETGRTLGDLLEVKGVAAGDRVVLRPDEHVRD
                     GERVRATKS"
     gene            22546..23238
                     /locus_tag="VI319_10970"
     CDS             22546..23238
                     /locus_tag="VI319_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864409.1"
                     /translation="MSEARAPLVEIRRLAKAYRRGGQAVPVLSDINLEIAEGDFLALM
                     GPSGSGKSTLLNLIAGIDKPDAGELRVAGEDITRLSEAELADWRAANVGFIFQFYNLM
                     PVLTAFENVELPLMLTRLARRERRERVDMMLSLVGLAERASHYPSELSGGQKQRVAIA
                     RAIVGDPRLIVADEPTGDLDRVSAAEILHLLQRLNRELGKTVIMVTHDANAASAARAV
                     IHLEKGELVAEP"
     gene            23235..24398
                     /locus_tag="VI319_10975"
     CDS             23235..24398
                     /locus_tag="VI319_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6864410.1"
                     /translation="MTAHVLKLIVKNALRHKLRTALTAAGIVVAILSFGLLQTVIGAW
                     YAGANAASDARLVTRNAISLAFPMPLSYASRIRAVDGVAAVTHANWFGGVYQDPKNFF
                     VQFAVDARTYLDLFPEFRLSEAERTAFVRDRKGAIVGRKLANQYGFKVGDSIPLRGTI
                     FPGQWEFVVRGIYDGGKPTTDTSQMLFQWDYLNESMLKTFPRRANQVGFYVVRIENPD
                     DAAAVSRAIDEQFKNSIAETVTETEKAFQLGFVSMSEAIVVAIRVVSFVVIVIILAVM
                     ANTMAMSARERLAEYATLKALGFGPAFLATLILGESVAICAAGAAAGIALTFPVAAAF
                     SAKMGTLFPVFHVEASTLVMQAVAALVVGLAAAVVPSLRSARISIVNGLRHAG"
     gene            24403..25575
                     /locus_tag="VI319_10980"
     CDS             24403..25575
                     /locus_tag="VI319_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6864411.1"
                     /translation="MASGIPVYYVARNLWTRRLTTGLTAGGMALVVFVFAVVLMLDEG
                     LKRTLVTTGEYDNVVVTRRASETEIQSLIQREQVPIVETHPAVALDRDGTALVSRELV
                     VLISLNKRNSEKPSNIAIRGVSPRALELRPQVKIVEGRMFEAGKSEIIAGSGITRRFS
                     GAGVGERLRFGQRDWTVVGLFDAGNSGFDSEIWGDVEQLAQAFRRTTYSAVIVRLADT
                     RRFDQLKRDIESDPRLTLEAKREQVYYADQSKALSTFISILGLTLSIIFSVGAMIGAM
                     ITMYASVANRVAEIGTLRALGFRRRAILAAFLMESMLLGLVGGGVGLFFASFMQLVTF
                     STMNFQSFAELAFGFTLNGAIVAKSVAFAAIMGLVGGVLPAARAARMKIVDSLRAA"
     gene            complement(25577..26566)
                     /locus_tag="VI319_10985"
     CDS             complement(25577..26566)
                     /locus_tag="VI319_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864412.1"
                     /translation="MRTRRVLAAIAIALAPLGAHAQSAPSASSGPGQGRVLHVIVPFP
                     AGSSPDLIARILTEKLAPALEHPVIVENRPGAGGNVGTAAVARAAPDGYTVGLSIQGP
                     LAVNKVLYKKLGYDPFEDLAPVTLVATSPNLLVVDPRLGVASVKDLVALAKKQPGKLN
                     YGSVGNGSASHLTMEMFKEAAGIDLVHVPYPGSPQVNAAIVAGDVAAAFVVPATAMPL
                     VQSGRLKALAVSTERKSAALPAYPSVAQSGFPGVVSVAWNAVVAPAATPQPTIDRLSR
                     ELRAIIHSDEVREKFLRLYFEPAGSSPEELGALMRSELERWRSIIAKTGASAD"
     gene            complement(26566..26877)
                     /locus_tag="VI319_10990"
     CDS             complement(26566..26877)
                     /locus_tag="VI319_10990"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY6864413.1"
                     /translation="MKIEICKLKEVAPGESRKIEVEGRAPIALFNVEGSLFCTDDTCT
                     HGGASLTEDGYLLDETVECSWHGGTFDVRTGEPTGAPCTERLKTYKVEIENDTVYAVL
                     E"
     gene            complement(26933..27721)
                     /gene="pcaD"
                     /locus_tag="VI319_10995"
     CDS             complement(26933..27721)
                     /gene="pcaD"
                     /locus_tag="VI319_10995"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEY6864414.1"
                     /translation="MKVKANGIQINYSVEGEGPWVVMSHSLACNLSMWDEQAAALSKR
                     YKVLRYDLRGHGGTDAPAGDYTFDMLAYDAHALLEALGIKSCHWVGLSIGGMIGQAFA
                     LKFPGVFETLVLADTTSRNPPEAKPVWEARVKTAREKGMEGVVEPTLQRWLTEPYRKS
                     HPETAARLAAMIRSTPVAGYAGCCAAIPTLNFTDRLKAIKVPTLVIVGEQDPGAPPEV
                     AKVIHQNISGSELVVIPSAAHIANLEQTAAFNRALLSFLDKHRA"
     gene            complement(27779..28780)
                     /locus_tag="VI319_11000"
     CDS             complement(27779..28780)
                     /locus_tag="VI319_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY6864415.1"
                     /translation="MPTPSSPRLEVAAWILAGLLLLLTLLLHLLPALLAGLLVFELVH
                     VIAPRLRLGKRGKVAAVAILATAIVAALSLALFGAVSFFRSDAGSLTALLQKMAEIID
                     GSRSHLPGWVLQYLPTDAEELRQALTDWLREHAGEVQLLGKEAGRLSAHLLIGMIIGA
                     MVSLREAQPSRHGAPLARALGQRAALLGDGFRRIVFAQVRIAAINTAFTAIYLGVALP
                     LLGVHLPFAKSLILITFVAGLLPVIGNLLSNTIIVVVSLSASLAVAIASLAFLVVIHK
                     LEYFLNARIVGSQIRASAWELLLAMLVMESAFGLAGLVAAPVYYAYLKEELSARALI"
     gene            28848..29870
                     /gene="galE"
                     /locus_tag="VI319_11005"
     CDS             28848..29870
                     /gene="galE"
                     /locus_tag="VI319_11005"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY6864416.1"
                     /translation="MPDSVLVTGGAGFIGSHACVELALAGYDVTVVDNLANSRRSVIG
                     RVSRIAGRPIRFAEGDVRDKDFLRAVFAARRFSAVVHFAGLKAVGESVAEPLRYWEHN
                     VGGTLTLLAAMAEHGVRRIVFSSSATVYGQSAAMPLAEDAPLGPTNPYGRTKLAIEQI
                     LGDVARADAQWRAVLLRYFNPVGAHAGGTIGEDPDGVPNNLFPYVAGVAAGRFERVPV
                     FGNDYPTPDGTGVRDYIHVMDLAAGHVAALRALDALPAVTAVNLGTGRGYSVLEAIAA
                     FERASGRKIPFRVEPRRPGDVAMSYADPSLAARLLAWKAVRGLDDMCADAWRWQQWRA
                     AHEAQL"
     gene            complement(29878..31995)
                     /locus_tag="VI319_11010"
     CDS             complement(29878..31995)
                     /locus_tag="VI319_11010"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY6864417.1"
                     /translation="MAAVAITDLRQHLGYLKPSDVSRIEEAYQFSSAAHEGQFRKSGH
                     PYISHPIAVAEIIADWQLDAQAVMAALLHDVMEDTEVSKGEISQRFGKPVAELVDGLS
                     KLDRIEFQSQQDAQAENFRKMLLAMARDVRVMLIKLADRLHNMRTLDAVDTQKRRRVA
                     RETLEIYAPIANRLGLDSLYREMQELAFSHLYPLRYKTLAKAVKAARGNRRQVVNKIL
                     EAVKERLPAAGIEANVTGREKHIYGIYKKMLEKHLSFSQVLDIYGFRVIVKDVPSCYL
                     ALGALHGLYKPVPGKFKDYIAIPKFNGYQSLHTTLIGPYGTPVEIQVRTQAMHRIAET
                     GVASHWLYKSTDAEINEVQKRTHKWLQSLLDIDHAAPDAAEFLEHIKVDLFPDEVYVF
                     TPKGKIMALPRGATAVDFAYMVHTDIGNRCVAARINYELAPLRTELKNGDRVEIITAA
                     HAHPNPAWLTYVKTAKARFQIRHFLKTMQIEEAAMLGERMLAQAVRALGTPHAEPTPE
                     RWEKLVRELGARSRQEIVADIGLGKRLAAIVARKLLALSDAHPSEAKFAAGSIVIHGS
                     EGMAVQFAKCCRPVPGDPIIGFIKKGQGLVVHTHDCTTAAKARGDPDKWLDVQWAPHT
                     DKLFDVAVRVIVVNQPGVLAKVAAAIAEAGSNIDNVSMDEERSLYTTMHFTLQVANRM
                     HLARVMRSLRRIQEVVRIARVKE"
     gene            complement(32047..32256)
                     /gene="rpoZ"
                     /locus_tag="VI319_11015"
     CDS             complement(32047..32256)
                     /gene="rpoZ"
                     /locus_tag="VI319_11015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY6864418.1"
                     /translation="MARITVEDCLKRIPNRFQLTLAATYRARQLAQGGTPLIEANRDK
                     PTVIALREVATGKVGIEYLSKVNTA"
     gene            complement(32270..32884)
                     /gene="gmk"
                     /locus_tag="VI319_11020"
     CDS             complement(32270..32884)
                     /gene="gmk"
                     /locus_tag="VI319_11020"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY6864419.1"
                     /translation="MSGDLFIISAPSGAGKSSLVSALLAQDRNLALSVSYTTRAPRPS
                     EQEGREYHFVDAGAFAAMLERGEFLESAQVHGNRYGTSRKWIADARGRGLDIILEIDW
                     QGARQVRKAFPEAVSVFVLPPSPALAELERRLRKRGHDDEEAIARRLANAREEMAHAA
                     EFEYVIINKDFEEAKRDLSAIVRASRLTAERQRARHPEAFQRPD"
     gene            complement(32893..33753)
                     /locus_tag="VI319_11025"
     CDS             complement(32893..33753)
                     /locus_tag="VI319_11025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY6864420.1"
                     /translation="MIHSMTGYASSARDLPQCSLAAELKSVNSRYLDIQFRLPDEMRA
                     LEPALREMIAARVSRGKVECRVAVTAAAGGAPSLALNETLLSALAEASRRVREAIPEA
                     APLRVGEVLHWPGVLGDDRSALVRDTALALVEQLLAEFAATRAREGGKLAGMLLERIE
                     RMERLLAEVQPLLPEAVAAYEERLAGRLREALGASDEERIRQEIALFGVKIDVAEELT
                     RLAAHLHEVRRVVNAGGVVGKRLDFLMQELNREANTLASKSVSKEVSDASLEFKLLIE
                     QMREQIQNIE"
     gene            33908..34855
                     /locus_tag="VI319_11030"
     CDS             33908..34855
                     /locus_tag="VI319_11030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6864421.1"
                     /translation="MPQQANQPLPNGYLLQNYRIASVLSCGGFSIVYLAYDENDQPVA
                     IKEYLPSQLALRKEGDALPSISDDNLATFRYGMKCFFEEGRSLARLSHPNVVRVLNFF
                     RANETVYMVMRYERGRTLQEHIQARRGSIKENFIRHVFALLLNGLREVHSNKLLHLDI
                     KPANIYIRNDGSPVLIDFGAARQTLAEEGLKLNPMYTPGFAPPEQYRNRELLGPWSDV
                     YAIGASMFACLAGVAPQAADQRLEKDRYVSATKTWPGKYSRHLLETIDWCLELDHLNR
                     PQSVFALQKVLLREKEPEVRRKRSLVDHVRGAFLKIARR"
     gene            34852..35766
                     /locus_tag="VI319_11035"
     CDS             34852..35766
                     /locus_tag="VI319_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY6864422.1"
                     /translation="MKFSIYQESRKGGRKANQDRIGYSYTRDSLLMMLADGMGGHLHG
                     EVASQIAVQFLTEAFQREARPRLADPLQFLSKSIVNAHHALVDYAKVRQLTDTPRTTC
                     VACVVQEGIAYWAHVGDSRLYHLREGKIQAQTRDHSRVQMLVDSGRIREEAVSAHPDR
                     NKIFNCLGAHTPPQVELSHKTVLRTGDTLLLCSDGLWGPLSGKIIAAALLTGDVLHAV
                     PELLTQAEQRAGKDADNLSVVAMNWTDSVQVQSPGEVSTQTLELDAVITQVEDFKKED
                     HSDLTDEEIERAIGEIRDAIRKHSPQKP"
     gene            35763..36485
                     /gene="rph"
                     /locus_tag="VI319_11040"
     CDS             35763..36485
                     /gene="rph"
                     /locus_tag="VI319_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY6864423.1"
                     /translation="MSTRPSQRRPDELRAVSLRRAYTRHAEGSVLVEFGDTRVLCTAS
                     VEEKVPPFLRDRGQGWVTAEYGMLPRSTHTRTDREAARGRQSGRTLEIQRLIGRSLRA
                     VTDLELLGERTITVDCDVIQADGGTRTAAITGGCVALYDALSTLEQRGLVKAHPARDF
                     VAAVSVGVYDGVPVLDLDYAEDSACDTDMNVVMTGSGGFVEVQGTAEGAPFTRAQLDA
                     LLELARAGIAQLVARQKQALGV"
     gene            36521..>36652
                     /locus_tag="VI319_11045"
     CDS             36521..>36652
                     /locus_tag="VI319_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY6864424.1"
                     /translation="MKKLVLASSNPGKLREIEALLAPLGVEVVPQSRLGIGDAEEPHA
                     "
BASE COUNT         5559 a        12499 c        12923 g         5671 t
ORIGIN      
        1 cgagccgaac gatctcgcgg tccgaccggg tgtcgcgggt ctccgcgacc gataagggaa
       61 cgacgctcgc gagcacggtc aggacgccgg ccgcggtcaa cagcacctgc gatggcttgt
      121 acatggacga cctcgcgtga gggagaggga ctgggactgt cgcctgcgtt agagggcgcg
      181 ccggggcgcc ggttttcgcg gcggcacgcg ggcgccggca ctagttcacg ccgggcggtg
      241 cgcaggcgcg tgcgcgacgt cgtcgcaggg cgacagcggc gcggttacaa tgcgggcccc
      301 gggatcgacg acatggaccg ctctccgcag cagctcgcgc gcaacgtaac gcgcctttcg
      361 cggctcgacc ttccgggcgc gggccaggtc tgcgtcgcag gcgactccgc ctacgtcggc
      421 catatcccga acacgaagcg cctgggcacc tcgatcctcg acgtctcgga cccgcgcagc
      481 ccgcgcgtgg tgtcgcagat ctttctcgac gaccccgaca cgcacagcca caaggcgcgc
      541 gtgatcgggg acgtcatgat cgtgaatgcc gagcgcaaca tgacggcgat cggccgcaag
      601 gccgagcagc tgccgctggc gcgcgcggag ctgcgcgccg cgctcgggcg cgagccgacg
      661 cacgaggagc tcgcggcgaa gatcgggatc gccgccgccg acattcccgc ggtcgagcac
      721 gccgagcgca acccgtacgg gggcggcggc tttcgcgtct acgacgtctc ggaccgcgcg
      781 aagccgaggc tgatcgggtt ccagcggacc ggaggcatcg gcgtgcaccg cttcgacatg
      841 gacacgcgct acgcctacat ctcgaccgag atggcgggct acgtcggcaa catcctcgtc
      901 atctacgaca tcgccgaccc ggcgcggccg cgcgaagtgg cgcgctggtg gatgccgggc
      961 cagcatctcg cgggcggcga gacgccggca tgggtcggca agcgtaaccg cctgcaccac
     1021 gcgctgcgca gcggcgatcg cctctgggcc ggctgctggc aggccgggtt gcgcgtgatc
     1081 gacgtctccg acatcggcgc cccgcgcacg atcggcgagt acaactatca cccgccgtac
     1141 ccggagccct cgcacacgtt catggacgtg ccctttcccg tcgccggccg caggatcgcg
     1201 ctcgcgatcg acgaggagga ccacgctgcg agccgcgacg agatggagcg ccgccgcggc
     1261 cggccgcacg cctgtctgtg ggtgttcgac gtgagcgacg tcgccgacat gaagccgctc
     1321 gcacgctacg aggtgagcga gctcgactcg ccctggagcc gcgccgcccc ggggcgcttc
     1381 ggcgcgcacc agttccgcga gaagatggac gggacgctgg tctactgcgc ctggttcgca
     1441 ggcggcctgc gcgtcgtcga cgtcgccgac ccgctcgcgc cgcgcgaggt cggcttcttc
     1501 atcccggagc cggcgccggg ccgggtcgcg ccgcagacga acgacgtcga cctcgacggc
     1561 cgcggtctga tctacatcgt cgaccgctac gccgggctcg acgtcctcga gctcactcca
     1621 ccccgttagc ggcgaagtcg cgcttcgcgg ccggcgtgcc gagatagcgc acgaacgcgc
     1681 gcgccacgtc cgaggaagcg gccgcgaccg ccgagtagct cgtgtagttc tgaacttcgg
     1741 ccggcagcgg gccgacgagc ttcagccctt tcggctcgaa cagcctgatc tcggtcatgg
     1801 cgccgaaacc gatctcgttg ccccggcctt tgatcacgtg ctcgaggacc tgcgcgccgt
     1861 tcgcgtagcg cgtggtcttc ggtttcagct gctcgccgat tcccatccgg tcgaacagcc
     1921 tctccagata caggccggaa gacgcctggt tgtagacgac cgagtcggcg cgcagcagcg
     1981 cctgcctcag ctcgtcgacg ctgccgatcg cgggcgccgg cgcgccggag cgcaccgtga
     2041 taccgacacc cacgcggccc gcggggacgt gtccttcgcc gctcactttg cccgccttcg
     2101 cgagctcgtc gagcacggcg gggggcgcga tcacgacgtc gaagcgctcg ccctcggcaa
     2161 cgcgcttgcg gatctgcggc gctgtgttga aggtgacgtg gaccttgtgg ccggactgct
     2221 tttcgaacgc cgcagcgacg gcttcgagcc ccggctcgat cgcaccgccg ctgatgactt
     2281 tgatctcggc cgcctcgctc gccgcggcga ctccggcgac caggcacatt gcgagccatt
     2341 ccttcatcgt ccgtctcctc gctcagtcgg cgcgcgcgcc ggagtccttg accaccttgc
     2401 tccatttcgg gatctccgcc gcgatgtact gcgcgaagtc gctcggcgtg cccgaggcga
     2461 cctcggcgcc cagcgactcg agccgctcgc gcaggtcgcg ctcgccgagc gccgcgacga
     2521 tgtcgcggtt caggcgctcg acgagcgcgg ccggggtcgc ggccggcacg acgacgccgt
     2581 gccaggtgat cgcctcgaat cccggcacgc ccgcctcggc gaccgtcgga aggtccggca
     2641 cgctcggcga gcgccgggcc gtcgtcacgg cgagcgcgcg cagtcgcccg gccctgatgt
     2701 acgggatcac cgatgccgac gtggcgaaca tgagctgcgc ctggccggcg atgacgtcgg
     2761 tgagcgcggg ctgcgcgccc ttgtacggca cgtgcaccat gtcgacgccc gtcatggcct
     2821 tgaacagctc ggccgcgagg tgcgcggcgg cgccgctgcc cgaggacgcg tagttgagct
     2881 gaccgggctt cgatttcgcg agcgcgatca actcgcgcac cgagcggacg gggagctgcg
     2941 gattgaccac caggatattg ggctggatcg cgaccagcgc gaccggcgcg aaatccttct
     3001 ccgggtcgta ggcgagggag cggtacaggc tccggttggt cgcgaggatc gagttgttgc
     3061 cgagcagcca ggtgtagccg tcggcgggcg cgcgcgcgac cgcctcggcg gcgacgttgc
     3121 cgcccgcacc gggccggttc tcgacgacga cctgagagcg ccacgtgtcg gcgagcttct
     3181 gcccgagcgc gcgtgccagg atgtcgctcg gcccgccggg cgtgaagccg acgacgaagc
     3241 gcaccggctt ctggggaaac gcgccctgcg cgagcgcgcc cgcggacgcg agggcgagga
     3301 cgaggacggc gagcgggcgc gagcggggca tgtccttatc atatcgggcg cgggtagaat
     3361 gcgctgcgaa gatggccggc cgaactccgc cgcaagtttc cgaagtgatg cgcacgctcg
     3421 ccgcgtacat cgcgcgcgcg ccgcgccgcg cgcttcctgc ggcggtcgcc gagaaaacca
     3481 agcaccacct cctcgatacc ctcgcggcca tgatctccgg ctcgcgcctg ctgcccggga
     3541 ggcaggcgat cgccttcgcc aggacgcagg gcggcgcgaa agaagcctgc atcgcgggca
     3601 gccgcatcgt caccagcgcg gtgaacgccg cgctcgcgaa cgggatgctc gcgcacgccg
     3661 acgagaccga cgactcgcac gcggcctcgc tcacgcatcc cgggtgcggc gtggtgccgg
     3721 gagcgctcgc catggcggag cgcgagcggg ccggcggcac ggcgttgctg cgcgcggtgg
     3781 cgctcggtta cgacgtgtgc gcgcggctca cgctgtcgct cgatccgtac cagttccgcg
     3841 aggacgggca ctcgacgcac agcttcgggc cgacgttcgg cgcggccgcg gcggcgggcg
     3901 cgctcgtcgg gctgcgcgag ccgcaggtgc gccacctgct ctcgtacgcg gcgcagcagg
     3961 cgtcgggtgt ctcgtgctgg atgcgcgacg agcggcacgt cgagaaggcc ttcgacttcg
     4021 gcggcatgcc ggcgcgcaac ggcgtggcgg cggccgcgat ggtggcgcac ggcttcaccg
     4081 gcgtcgacga tgtgttctcc ggcgagcgca gcttcttcgt cgcgtacggc cgcaagccgg
     4141 acccggcgcg cctcgcagcg ggcctgggcg agacctggga gatcatgaat gcgtcgatca
     4201 agcgctggtc ggtcggctcg ccgatccagg cgccgctcga tgcgctcgcg ctgctgatcg
     4261 ccgagcacgg cgtgcgcgcc gccgacatcg agcgcgtggt cgtgcgcgtc gcgcaccagg
     4321 gcgcgaatac cgtgaacgat cgcgcgatgc ccgatatctg cctgcagcac ttgtgcgcgc
     4381 tgatgctgat cgaccgcgac gtcagcttcc gctcctcgca cgacgcgaag cgcatgcgcg
     4441 acccgaaggt gctctcgctg cgcgcgcgcg tcgagctgca cggggacgac gcgctgtcgc
     4501 gcgcgatgcc gagccggcag ggcatcgtcg agctgcgctt gcgcgacggg cggacgctcc
     4561 gtcaccacgc gaccgaagtg cgcggcacgc ccggcaatcc catgacccgc gccgaggtcc
     4621 acgccaaggc gctcgacctc atcgcaccgg tgaccggacg cgcgcgcgcc gagcgactct
     4681 gtgatacggt ttggaatatc gagcggctcc gggacgttcg tgccctgagg ccgctccttg
     4741 ctcatcgaca ggagaccccc gcatgaatcg catcgtccat atcgcgctca aggtcgacga
     4801 cctcgagcgc agccgtcagt tctacgagaa catcttcggc ttcagggaga acaagaccga
     4861 gaaggtacgc gaccacacgt cgtgccacct ctccgacggc gcgatcgacc tctcgatcat
     4921 gcagtacgac gcgggcacgc agtcggcgga atcgaaggcc gcgggcgaag gtccctgcat
     4981 ccaccatttc gcggtggagg tgaacgacat ggcagccgcg gagcgtcaga tccgggcctt
     5041 cggctgcgag atcatcagcg acccgggcgt ggtccccgtg aagttcagag caccgggcgg
     5101 caccgtgtgc gagatcgtgc ccaagctccg ctaccaaaag atcaggaagg cgggccccaa
     5161 ggcgaaggcg aagtccaagg caaaggcgaa agcgaaagcg cgcaagggcg cgaagaagaa
     5221 gtagaaggcg gcgccgagga ggaggagcat ggaagagacg tcggagaaat cctccgactt
     5281 gtgggccggg ctcgcgctcg cggcgctcgg cgtctacatc gtcgtccagg ccgcccagtg
     5341 ggagtacatc gggcccgacg ggccgggccc gggtttcttc cccttgtggt acggcatcgc
     5401 gatggtggtc ctgtccgcgg cgctggtctt cgtcggcgta cgggcgcgcc gcgcgcgggc
     5461 tgcggccgcg agcgcgctgt ttccgtgggc gcggatcggg cgcgcgctgc tggtgtggct
     5521 cgcgttcgcc gtgtccgtcg cgctctccga ggtgctcggc ttcacgatca gcttcggcct
     5581 cctgtgcttc ttcatcgtcg cggtgatgta ccggcggcct ctcaaggtcg cggcggcggt
     5641 ggcggtcgcg agcgccctcg gcttctacac ggtgttcccg ctggcgctcg gcgtatcgct
     5701 gcccacgggt tgggtcgggt tctagcatgg aggtcctctc gggactcgcc cacggcttcg
     5761 gcgtcgcgct cgagccggcg aacctgctct ggtgcttcgt cggggtcttc ctcggcacgg
     5821 tggtcggcgt gctgccgggg ctcggaccgc cggcgaccgt cgcgatgctg ctcccgctga
     5881 ccttccacat gaacccggcg ggcgcgatca tcatgctcgc cggcatctac tacggtgcga
     5941 agtacggcgg gtcgacgacg tccatcctga tgaacgtgcc gggcgagtcg gcgtccgtca
     6001 tcacctgcat cgacggctat cagatggcgc gcaagggccg tgccggcgcc gcgctcggca
     6061 tcgcggccat cgcgtccttc atcgcgggca ccgtgggcgt ggtggcgctg atgctggtcg
     6121 cgccgccggt cgcgcgcttc gcgctctcgt tcagctcgcc ggaatatttc gggctgatgt
     6181 cgctcggcct ggcgctggtc gtgctgctcg ccggccactc gctgctgaag gcgctgctcg
     6241 cggcgctgct cggactgtgg atcgcgtcga tcggcaccga cctgttcagc gccacgtcgc
     6301 gcttcacgtt cgggcgcgtc gagctgcttt ccggcgtcga tttcatcgtg gtcgcgatcg
     6361 gcgtgttcgc gctcggcgag gtcttcgcca acatggagaa ggaggacgac gcgcagctcc
     6421 tgcccgtgcc gaaagggctg aggaacctgc tgccgacgat gcaggacatg aaggactgcc
     6481 gcttcgcgtt cctgaacggc tcggtcgtcg gcttcctcgt gggggcgctc cccgggggcg
     6541 gctcgacgat cgcgtcgttc ctgtcctacg ggctggagaa ggcggtgtcg cggcgccgcg
     6601 agcagttcgg caccggcgtg atcgagggcg tggccgcgcc cgaaggcgcg aacaactcgg
     6661 agacgggcgg cgcgctcgtg ccgctgctca cgctcggcat cccgggctcg ggcacgaccg
     6721 cgatcctgct cgcggcgctc gtgctctggg gcttcaagcc cgggccgctg ttcatcccgg
     6781 agaacccggc gctcttctgg ggactggtcg cgagcatgta catcggcaac gtaatgctgc
     6841 tcgtgctcaa cctgccgctc gtgccggtgt tcgcgcaggt gctgaggacg ccgacgtata
     6901 tcctgttccc gatcatcctc ggcgtgtcgc tggtgggcgt ctacagcgtc tcgggcagca
     6961 tgttcgacct gctgctcctg tgcttcttcg gactgctcgg ctacctcatg cgcaagctcg
     7021 atttccccgc ggcgccgctg atcctcgggc tcgcgctcgg cgcgggcatg gagcgcgcgc
     7081 tccggcaatc gctgatgatg tcgggcggcc agctctcgat tctcgtgtcg cggccgatct
     7141 cggccgcgat gctggcgctg gcattggtgg tcctgctcat cccgctcgtc ggcaaggcga
     7201 acgcctggcg cgtgcgggcg atcgaccagg aacaggcctg aggaaaagga ggaagcacga
     7261 tgagaaagta ctggtattac gcaatcgcgg cggcgctcgc ctgcgccgcc accgcgcacg
     7321 cgcagggcta caagccgacg cggccggtgg agctggtggt gcacaccggc ccgggcggcg
     7381 gcagcgacgt gctcgcgcgc gcggtcgccg tcatgttcga gcgagagaag ctggtcccgg
     7441 tgcgcgtgaa cgtcgtcaac aagaccggcg gcaacggcaa cgtcgcgatc gcgtacctgg
     7501 ccgagaagaa gggcgacctg aacaccatcg ggttcttcac cggggctcag ctcaccagct
     7561 acctggtcag caaggaagcg caggtcacga tacgcgacct cacgccggtc gcgcgcctgg
     7621 tgctcgagcc ggcggtcatc gccgtcaaag ccgactcgcc gtacaagacg ctgcgcgatt
     7681 tcgtcgacgc ggctaagaag aacccgggcc agctcaagca gtcgggcggc tcgatcacga
     7741 gccgcgacaa cgtcgtgcgc gagctgctgc agaaggcttc gggcgcccgc tgggcctaca
     7801 tctcgtttcc gggcggcggc gagcgcattg cggcgctcct gggcggtcac gtcaacatga
     7861 tggtgatcga gcccgaggag gcgggcgagc acatccgcgc gggcaacatg cgggtgctcg
     7921 cgcaggtctc ggaaaagcgt ctcgcgcgct tcccgaacgt gccgacgctc aaggaggcgg
     7981 gtttcgacgt gccggtggtg ccgcaggtgc gcggcgtcgt ggcgccgccc ggcatcccgg
     8041 aggcgaacgt gcgctactgg gaggacgtgt tcaagcgcct gacccagacc gcctcgttca
     8101 ggaagtacgt cgaggaaaac cagttcgaga tgggctacct cgacggcgcc ggcctcgtga
     8161 agttcatgga cagctacttc gaccagatgc gctcgatcct gaaggacgcc ggggtcaaga
     8221 ccgtgcgctg atgggtgtag cggccgattc cgtcgccgag cggctcgccg caggggtcgc
     8281 cgggctgtcc gcgtccggga ttccgcgcgg cgtgcgcgag acctgcgcgg ccctgctggt
     8341 cgatgtcggc ggcctgtgcg tcgccgcgcg cgcgagcggc tacgtgcgca tgctcgccgc
     8401 cgcggtcgac cgcggcggac cgtgcaccgc gatcggccac ggcggcggcc tcgccgccga
     8461 ggacgcggcg atgatcaacg gcacggccgc gcacggcgag gatttcgacg acaccttcga
     8521 aggcgggccg gtgcacagcg gcgcggtcgt cgttcccgcg gtgctcgcgg cctgcgagcg
     8581 cttcggacgc ggcggcgact cggccctcct gggcatcgct gcgggcgtgg agacgctgtg
     8641 ccggctgagc ctggtcgccc cgaagcgcat ccacaaggcc gggttccatc cgaccagcgt
     8701 gctcggcgcc atggccgcga ccgcgggcgt gggcgcggcg ctcgggctcg accgccggca
     8761 gacggtcgac gcgctcggca tcgccggcag catggcgggc gggatcatcg agtatctcgc
     8821 cgagggcgcg tggaccaagc gcctgcacgc gggctgggcg gcgcgcatcg gcctgcacgc
     8881 cgcgcgcatc ggccgcgagg gcttcctcgg tccgcgcacg gtgttcgaag gcacgcacgg
     8941 gctgttcttc gggttctccc attccgcgga aggggactac gacgccctgg tgcgcggctt
     9001 cggccgcgac tgggtgatga cgggaatcac cttcaagccg tacgcgaccg gcaccatgaa
     9061 ccagccctac atcgactgcg cgctgcgcct cgcgcgcaag ggcttcgctg ccgaggacgt
     9121 cgccgaggtg ctgtgcgaga cggcggaagg atacgtccac cgcctgtggg agccgctcgc
     9181 ggccaagcac cgccccccga acgactacgc cgcgaagttc agcacgccgt tcaacatcgc
     9241 ggtcgcgttc ctcacgggcg gggcggggtt cgaggccttc accgagcgca ccgtgcgcga
     9301 cccgcgcatc ctcgcgctcg cgtcgaaggt gcgctacgtc gtcgacgcga acaatcccta
     9361 tccgaaggcc tacaccggcc acgtgcgcgt gacgctcaag gatggccgcg tcttcgagga
     9421 gcgccagccg cacatccgcg gcggcgtgca ggagccgctc acgcgcgagg atctcgagga
     9481 caagttccgc ggcaacgccc gccacggcgg atgggaggac gcgctcgccg atcgctatct
     9541 gcaattcgca cggaccgcgt tcgacgcggc gtctctcgcc ctgggcgagt tcggggcctg
     9601 atgcccggag cgcggcgcgc gagcattttt ccacgcttgc gcctctcggc cgacgggggt
     9661 cggacgagcc atatgtttac acaaaactat tgggtcgcga tatgatttcc cgggagttca
     9721 tcagaacgga ccgccgcggg ccgatccgac ggcgggaccc gtaacaacac aggaggagaa
     9781 catgaagctc cagtctcagc tgagcatcgc cgcttcgctg ctagcgctgg cgttcgtcgc
     9841 gggctgcgcg agcgaggcaa agaaggatgc gacgtccgcg tcgcccgccg cgtcgtcgcc
     9901 ttcgtcgtcc ggcgccgcgc cctcggcgca gtcggcgcag ccctcgggcg tgaccggaaa
     9961 cgcgctcaac gatccgaaga gcatgctgtt caagcgcagc gtctactacg agttcgacca
    10021 gtcgaacgtg aagaccgact accgctcgct ggtcgaggcg cacgcgcgct acctgcgcga
    10081 gcatcccggc gcgaccatca ccatcgaggg caactgcgac gagcgcggca gccgcgagta
    10141 caacctggcg ctcggccagc gtcgcgcgga cggcgtgaag agcatgatga cggtgctcgg
    10201 cgtgccggac cggcaggtcg acacggtgag ctacggcgag gagaagccga aagcccaggg
    10261 ccacagcgaa gacgcttggt cgcagaaccg ccgcagcgat atcgtctacc gcaaagtcca
    10321 gtaatccacg ggtcatcccg aaaaagcggg ggtttccccc gctttttttt cgtctctcgg
    10381 tggcacctgc gccgcatgct atgctccgcc gttgagccag aacaggcccg ggaatccacc
    10441 cgggcattgc attccggctg tgtttcgctc gtcccggggg ggaccggatg gctctggcgt
    10501 tggtgccggc gcacaggctc gcgcgcatca attacgcgcc gcgcgcgctc gcgttcgcct
    10561 acaccttcct ggtgctcgag gcgctcatgc tcgagcgcgg ctcgggcggc tggatgctgg
    10621 cgctcggcgc gctgcagttc ctcgtctacc cgcacctcgc gtacctgcac gcgcgcctgg
    10681 tcccggagtc caagcgcgcc gagctgatca atctggcgtt cgacgccctc accctcgggg
    10741 cctggaccgc gcaggcgcag ttcgcgctgt ggtggagtgc gggcctcctg tcggcgatca
    10801 gcctgaacag cgccgcgaac ggcgggctga ggtacctcgg tctcgcctgg gcgctgttcg
    10861 ccgcgggcgc ggcgctgtgg ggaggcattt ccggcttctc cttctcgccc gacaccgggc
    10921 cgctggtgac ggggctgtgc atcgccggtg tcatcgtgta cgtctcgtgg atcggcacca
    10981 tcatgcgcgc gcagaaccgt gcgctcatgc gcacccgcgg cgcgctgcac tcgagcgagg
    11041 accagttccg cttcatcgcc gagcacgcgg gcgacctggt cgccgtcctc gacccgcgcg
    11101 ggcgcatccg ctacaccagc gcctcgcacc tcgagcactt cgaggccgcg ctcgtcgctc
    11161 ctgggcagga ctgggtgcgg ctcgtgcatc ccgacgaccg cgaacgcgcg cgcaatttcc
    11221 tgcagtacct ggtgatgtcg cgccagatgg agcgcatgag cctgcgcctg gtcccgatgg
    11281 acgggccctc gctggtggtg gattgcgagg gcaaccccgc ggcggacgac agcgacggca
    11341 tgggaatgat catcctggtt atgcgcgaca tcacgacgcg cgtgcgcacc gagatggacc
    11401 tgcggctcgc ggcgcacgcg ttcgaccgcg tctccgacgg cgtgctcatc agcgacggct
    11461 ccggcaggat cgagtacgtc aacggcgcct attgcacgat gacgggctac gagccccgcg
    11521 atctcgtggg gcgcaccacc gacgaggtgc gctcggcgct gaagggcgac gagctctacc
    11581 acgagatctg gcgctcggtg cagcggaccg ggtcctggcg aggccgcttc atggagcagc
    11641 ggcgcgacgg caagttcttc gcggcgtcgg tggtcgtttc cgccgtgcgc gacaaggagg
    11701 gcgcgtcgac gcactacgtc tgggtcatca ccgacccggc cgcgcccgag cgcgcggctc
    11761 gcagcgcctg agccggtgta gcgccgtcag ccgtcccggc gacgagagca cgcagagccg
    11821 ctggccggcg ccgcggccgg aatagaatcg aaccccgccg cgtttctccg gactaagcac
    11881 aacggcatcc aaccggaaag gagtgaaaaa tggccggcac ccacatcgac atccgcgccg
    11941 ccgacggcgg caagttccgc ggctacctgt gcctgccccg atcgggcaag ggcccggggc
    12001 tggtcgtgga gcaggaaatc ttcggcatca acgagaacgt gcggctcgtg tgcgagcact
    12061 acgcgaacca gggcttcgtc gcgctcgccc ccgacctctt ctggcggctg cagccgaacg
    12121 tggacctggg ctattcaccc gaggaatggc agaaggcgtt cgacttcatg aagcgcttcg
    12181 acatcgacaa gggcatcgag gacgtcgggg cgaccatcaa gcacttgcgc gggatgaagg
    12241 aatgcaccgg cagggtcggc gcgatcggct attgcctcgg cggcaagctc gcgtacctga
    12301 ccgccgcgcg caccgacgtc gactgcgcgg tcgcgtatta cggcgtgggc ctcgatcaga
    12361 acctgaacga aaagggcaag atcggcaagc ccctcatgat gcacatcgcc gagaaggaca
    12421 agttcgtgcc gcccgaggcg caggcgaaga tcaaggcggg cttgaaggac atgaagaacg
    12481 tggagatcca cagctacccg gacgtcgatc acgccttcgc gcgcatcaac ggggaccatt
    12541 tcgacgcgaa cgccgccgag ctcgcgaaca agcgcacgct cgacttcctg aagaagcacc
    12601 tgatggggcg ctagcgctcc gccgctttct tctccagccg gatctgcgcc gcgcgggcac
    12661 agccctgcat gacctggaag tagcgctgca cccagtcggc gccgatctcg taggggtgcg
    12721 gccccttgcc ccgcccggcg agcatgcggt tcttgttgta ggcgttgtcg aactcggagt
    12781 ggttcgaaag cagcacggtc gcgcccgtct ccgcggcctt ggccgccatg tgccgctgcg
    12841 agtcgatgta gatctggaag ttcttgatgc ccgggtcggg agtgtcgttc acgaagttga
    12901 acgcggtgcc gccggaatag gcgacattca cgggccggcc gcggtcgtgc acggtgaacg
    12961 tgtaggacag cgttccgggc gtgtgtccgg gcgtgagcca caccgttagc gtcgtgtcgg
    13021 cgagcgtgat cttcatgccg tcggtcgcga cgatgtcgcg cctgggcgcc atggtcttgt
    13081 accggttcgg atgccgctcg accaggtccc agtcgggccc gcccatgacc acgcgcgggt
    13141 ggtagcggcg ctggatcatc tcggcgccgc cgatgtggtc ggcgtgcgcg tgcgagatga
    13201 cgagatactt gatgtccttc gggtcgaggc cgagcttctc catcccgccg agaaccagct
    13261 cctccgagtt gtacgggtag atcgtgtcga tgaggatgag gccggcgcgc gttcgcagcg
    13321 cccacgacga atggatcttg ccgcccacga agtaaagctc gtcgaagacc ttgctcggct
    13381 cggcgtacca ggtctcccgc ggcggcgctt tcgctgggtc ggcgacgtac gggggcacct
    13441 tgtcgctcag gtcctcgccg ccgctctgcg gcagcaggca ggtgcgcgcc agcgtgccga
    13501 ggaactcgaa gcccgcggcg gtcttcgccg agcggatggc ggcgtcgatc tcggcgtcgt
    13561 agtcgcgctt cgccggctgc gcgagcgcgt ggccgcagag gcacgcggcg agaacgagca
    13621 ggccggcgag tcgaaccgcg cccatcgcta cacccatacc tgcagcacgt agtcgtacgc
    13681 cgcctgcgcg ttcgtcatgt cgacgcgctg cagcttgatg cggaagccgt cccgggtccg
    13741 cgtcaggtgg tgcgtgtaag cgccggcgaa atggcgcacg tcgtcgcggc gcagctccat
    13801 catgtggaag cgcgagcgca cgcagacgtc gccgttggcg agctcgcgct cgaggacgac
    13861 gttgccgatc acgtggttcg tgccccagag cggccgctgc gaccaggcgt cgggatgcag
    13921 cacgcgcttc atgcgcacgg tcatgaggtt cttgtcctcg gcgaagatgg cgggcatgcc
    13981 ttcccaggtc gcgtgcgagg gctcgggcgg catccagtac acgccgtcgt cggcgaagag
    14041 atcgatccag tcgtcccagc gcttctcgtc gagcagctcg gcctgacgat agaggaactg
    14101 ctccaccgcg tgctgcgtgt cgcggctgcc cgcggcgcgc cggcgcgggg cgggcttcgc
    14161 gaggtgccgc ttcgccgtct tcgccatcac cgctccgcgg tcatgtaccg cgcccatgcc
    14221 ttcatctggt tgcgcatcgg cgcctccgac gtgccgcagt tgggcgcggt ctcgacgcgg
    14281 ccgtcgcgct ccacgtcgcg gcccatgtgg cgggcgaagc tcacccaatc gccgccgtcg
    14341 gacgccaggc cctggtggca cttccagaag ttctcgaggt cgtccgcgtt gaccagcgtg
    14401 gcgggcgaat tgaccagatt gtagtacgcg agcgagcgtc ggtagatcgc ctcgggcgcg
    14461 cccttcagcc ggaagtgcca gatctcggtg agcgtcttgt cgacgccgag cgggcgcacg
    14521 gcgcgcagct gctgcagcgg cgactgcacc gacaatccgg ggtagatgag cacgtggtgg
    14581 atgttgacgc cgatgatctc ctcgaagcgc ttctttccgt acgcctcgat catgatcctg
    14641 tcgtaggcga gcgtgtccgg gtcctgcggt cgcaatccca tgtagccggt gaggatgcaa
    14701 tgcccgttcg gatagttgag cgtctggaag ccgtcccact tgtcgatggt cacggtcgcg
    14761 aaggccgaga gcatgtgata ggagagcggc gcctcgccct tcctggcctt cgcggcgatt
    14821 atcttttcga cctcgtgcgc ggcgcggccc gtggactggt gcgtcacgga aggatgcagc
    14881 gcgtcgagct ggttctcgag gaagatcttc cagttcgagt gctggatgac gcggaagcag
    14941 ttcggcacga tctcgacctc gccctcgggg gcgcggtcgc acatgtcgtc gaaggcgacg
    15001 cgcgactcgc cgagaaactc cgcgagcgtc gggccgtccg gcgcgaggct tgcgaaaacg
    15061 aagccgcggt agttctcgac gcgcgccgcg cgcttcatgc tgcagtcggg gttgtccttg
    15121 ccccagcgcg tgccggcgta gccggactcc tgcatcggga tcgagcgcag ccggccgtcg
    15181 tggtggaagg tccatgcatg gtaggagcag cggaagaact cgccggcgtt gccgctgcgg
    15241 tcgccgcaca tcatgttgcc gcggtgcggg cagcggttgt agaggacgtt cacgccgccg
    15301 tcgcccgcgc gaaccatgac catgggctgg cggccgacct gcaccgtgta gtagtcaccc
    15361 ggccggggga cctgcgactc gtgaccgcag tagatccaga cgcgctcgtg gatacgctcc
    15421 atctcgagct cgaagatcgc cgggtcggtg tagacctcgc gccgcacccg gtcgggctcg
    15481 acgtagttgc cgaggttggg gacgtgttcg tgaagcatgg agctccttct cgcggggcgc
    15541 ccgggggaag ggaacgatac cgtccgaagg acggattttc aaggcatgcg cccggacgga
    15601 cgaaccggac cgggcggcgg gcgagcgccc gcgcttactc gacgcgctgg atcaggtagt
    15661 tgtagggctt gccgaccgcg tcccagaacg cctcgatctt ggcagccatc ttcttgccga
    15721 cgaagccctc gaccagcagc gacaggccgt agaagaacac gatcgggaag atagccacgg
    15781 cgatgaacgc gatggcgacg gtgagcatca ggtagttctg gacgtgcttc atgaacatgg
    15841 cggggctccc cctgtctcgt atcgagcagt gtaggagcgg cgccttgcgc agacctgaca
    15901 aaacccgcat tacaccccga ggtaacgatg ccacagtgcc gggttcccgc cgagctgccg
    15961 cgatgagccg cgccaggcga cgcggccacg ctcgatcacg gtgtggtggt cggcgagctc
    16021 caccaggcgc tcgacgtatt tgtcgatgac gaggatcgtc tggtcgcccg ccttcagcga
    16081 cgcgaggcag cgccagatct cctcgcgcac caggggcgcg aggccctcgg tcgcctcgtc
    16141 gaggatcagc aggtgcggat tggtcatcag cgcgcggccg atcgcgagca tctgctgctc
    16201 gcctcccgag agctggctgc ccagatgtcc caggcgggcc gcaatccccg gaaaccggtc
    16261 gagcacgcgc tcgagcgtcc aggggtcgcg cgatccgcag cggttcgccg cgaaggcgac
    16321 caggttctcg cgcaccgaga gattggggaa aatttgccgg ccctcgggca cgagcgcgat
    16381 tcccgcccgc gcgatgcggt cgggcgagag gcgctcgatg cgctcgccgc ggaagcggat
    16441 cgagccgcgc cgcgccggcg tgagccccag gatcgagcgc accgtcgtgg tcttgcccat
    16501 gccgttgcgg cccagcagcg tcgccgcctc gcccgcgttc acgaccaggt ccacgccgaa
    16561 cagcacctgg ctcgcgccgt acgcggtctc caggccttcg atctccagca tcgcgctcac
    16621 gccgactcct cgccgagata ggccttgcgc acttgcgcgt cggcgcggat ctcggccggg
    16681 gtgccggtcg cgagcacgcg tcccgacacg agcaccgaga tgcggtcggc gagccggaac
    16741 accgcgtcca ggtcgtgctc gatgagcagg accgagagcg ccggttcggc ctcgcgcaac
    16801 tgctggatca gggccagcat gcgcgccgac tcctcgggcc ccatcccggc catgggctcg
    16861 tcgagcagca acagccgcgg ccgagtcgcg agggcgagcc cgacctcgag cgtgcgctgc
    16921 tcgccgtggg cgaggtgagc cgccgcgacc gattcgcgcc cggcgagccc gatgcgggcg
    16981 acgatcgcgg ccgcctgctc gtagagcgcc ttctcgcgcg cggcgggccg ccagaacgac
    17041 atgctcgacc ccgagcgcgc ctgcaccgcg agcgcgaggt tgtccaggac gccgagcgcg
    17101 ccgaaaagcg tcgtcacctg gtacgagcgc gcgaggccga gccgcacccg cccgctcggc
    17161 ccgagcgcgg taatgtcgcg ccccgcgagc tcgatgcgcc cggagtcggg ccggagcgcg
    17221 cccgcgagct gcgcgatgag cgtggtcttc ccggcgccgt tgggaccgat cagggcgtgg
    17281 atctcgcccg gcgagaccgt gagcgaaaca ccgtcgctgg cggggacgcc gccgaagcgc
    17341 ttgcgcagct cgatcgcggc gaggagcggc gccgtcatcg cgggcgccgc gcgagcagcc
    17401 cggcgatgcc ctggggcgcg aacagcacca cggcgagcag caccagtccc acggccaggc
    17461 ccgcgtgctg cgggtagttc ggcagcagcg ccgccaccca ggcgacgcgg tggttcgcga
    17521 ccaggtcctc gagcccgagc agcgccgtcg ctccgagcgc gccgccccag aggctgccga
    17581 cgccgccgat gatcaccatc accatcagcg agccggactg cgtccagtgc agcacgctcg
    17641 gattgacata cttgccgtgg ctcgcgagca gcgcgcccgc aagcccggcg gcggcgcccg
    17701 cgatgacgaa gcacacgagc ttgtagcggt agaccggaaa gccgatcgcc tcggcgcgcg
    17761 tctcgttctc gcggatcgcc cggatcacgc ggccgaagcg cgagtgcgcc aggcggtgca
    17821 gggcgacgag cgccgcggcg agcgcggcga gcgccacgta gtagaaggtc tgcgcgtcgt
    17881 cgaggtcgat gccgccgccc acgcgggcgc gcgcgggcag cgtgaggccc tcgtcgccgc
    17941 cgtacgcctt catcgagttt acgaggaagt acgtcatctg cgcgaacgcg agcgtgatca
    18001 tgatgaagta gacgccgcgt gtgcgcagcg ataccgcgcc gatcgcgagc gccgcgagcg
    18061 ccgaggcggc gatcgccgcc ggccagccga tccaggcgct ggcgacgccc tcggccagca
    18121 ggatcgacga gacgtaggcc cccgtgccga cgaaggcggc gtgcccgaag gagatcatgc
    18181 cgccgtagcc gaggaccagg ttgaggctgg tcgccgcgag cgcgtagatc aggatgcggc
    18241 ttgcgaacga aaggtagtac gcgtcgccct ccgcgcgcat cacgaacggg aacgcgagcg
    18301 ccgcggcgac caccaggccg agcaccaccg ccccgggccg caggcgggcc acgctagcct
    18361 cgcgccggga agaggccctg cggcctcagc gtcaggatca ccgccatgag caggtagacg
    18421 agcaccgagg cggtcgcggg cccggccgcg ctcgcgagct gcggggggag aaaggctccg
    18481 aaggcgatcg gcaggaacgc gcggccgatg gtgtccacca tgccgacgat gagcgatccg
    18541 accagcgccc cgcgcaccga cccgatgccg ccgatcacga ttacgacgaa ggcgacgatc
    18601 aggatgttct cgcccatgcc gacctgcacg gcgaggatcg gcccgagcag cgcgccggcg
    18661 acggcgcaaa gcccggcggc gatgccgaac accaccgtga acaggcgcgg gatatcggct
    18721 cccatcgcga gcgccatctc gcggttcgac gcgccggcgc gcacccgcat cccgatccgc
    18781 gtcttcgcga ccaggtagta gagcagcccg gcggccgcgg cgccgaccgc gatgatgagc
    18841 agccggtagg aggaatacag catgccgggg aagagctcca ccgggcccga gagcgcctcg
    18901 ggcgcggcaa gcggcaggtc ggtgccccag acgatgcgaa cggcctcgtt gaacacgagg
    18961 atcagcgcga acgtcgccag gacctgcgag aggtggtcgc gcgcgtacag ccgccgcagc
    19021 agcgacatct cgagcagcat gccgagcgcc gcggtcgccg gcacggcgag cagcacgccg
    19081 agcgcgaacg agcccgtgag cgagacgaag gtcgcggcca ggaacgcgcc gagcatgtag
    19141 agcgacccgt gcgcgaggtt gatcatgtcc atgatgccga acacgagcgt gagccccgcg
    19201 gcgagcagga acagcatcag gccgaactgc aggccgttca gcgcctgctc gaggatcagg
    19261 atcccggaca cgctacttca tcggacattc gtccacgtac gcgtcgccgc ggtccttgaa
    19321 gatcgtgccc gcggtcctgt tgaccaggcg tccctgcgcg tccttcgcga ccacgcgcaa
    19381 gtagtagttc tggatcgggt actggttgcg gttgaatttg aaggggccgc gcaccgagtc
    19441 gaacttcgcc gccttgagcg cgtggcgcag cgcctcgcga tcctcgacct tgcccttgac
    19501 gtcgcgaacg gccgcgtcga tgaggagcgc ggtgtcgtag ccctgcgagg cgtacacggt
    19561 cggcaggcgc ccgtactcct tctcgaacgc cgccacgaat ttccggttgg cggcgttgtc
    19621 gaggtcgagc gcccagtgcg cggcgtcgtg gatgccgagc atcggctcgc cgacggcgcg
    19681 gatgatgtcc tggtccgagg agaaaccggg cgtgatcagc tggatgtcgc gcgacaggcc
    19741 ggccgcgacg aattgcttga tgaagttgat tcccatgccg ccgggcagga agatgtacac
    19801 cgcctgcggc ttcgccgcgc gcacttgcgc gagctccgcg gagtaatcga gctgcccgag
    19861 ctgcgtgtag acctcgccga cgatgctgcc cttgtagaag cgcttgaagc ccgcgagcga
    19921 gtcctttccc gcctggtagt tcggcgcgag catgtagacc gccttgtagc cttgcgaggt
    19981 ggcgtactgc ccggcggcct cgtggtaggc gtcattgggc caggaggcgg cgaagaacca
    20041 cggattgcat tgcttgccgg cgagcggcga aggcgccgcg ttcggactga catacacgac
    20101 cttgttctcg aacgcgatcg gcaccgacgc gagcatgatg ttcgagaaca cgaagccggt
    20161 gaggatgtcg accttgtcga gcttgacgtt cttctccgcg agctgacgcc cgacgtcggg
    20221 cttgaactgg tcgtcgccga cgatcacgtc ggcgcgcagg ccgccgagct tgccgccgtt
    20281 catcttcacg gccagcatga aagcgtcgcg gatatcgatc ccgagcgcgg cgctcgggcc
    20341 cgaaagcgtg ctgatgaagc cgaccgtcac ctggtcggcc gcgcgcgcgg gcgcggccgg
    20401 aagcgcggcg aaagcgagcg ccgcggcgca gaacaccgtt gccttgttca tggaatccct
    20461 cccggatcag gcgaagacct tattggcccc gcggcgggaa gtcaagcagc gcggtttcct
    20521 gcacaatagg atccatggga gaagcacgaa cgcgaacggc ggccggcgcc gcgctgctcc
    20581 tcgtcgcgct ggtcgcggct tcggcggctc tcgcggagga cgcgcccgcc gattcgccgg
    20641 cggccgactg gtcggggcta tatctgggcg cccagttcgg cggcgacacg tcggacgcgc
    20701 gctggaggac cgatgccacc gtcgcggacg gcgacgccgt gcatcagcgg gcgagcagcc
    20761 cggcgctggg cctgcagctt ggcgcgcgtt ttcgcgtctc gcgccactgg ctggtcggtg
    20821 ccgagggctc gtatacgctg gcgcgactgc gcacggtcgc acagtcggcg gagctcgccg
    20881 agcgcggtct cccgggacgc tcgcgcgaga ccgacttgcg caatctctac gccgtcaccg
    20941 ttcaggcggg atatgcccac gaggaccggt ggctgttgta cggcaaggcg ggggtcgccg
    21001 ggtcctacgt ctatctcgcc accgcgaacg tgctgagcgg ggtcgtgtcc tcgaccaacg
    21061 gccgggcacg cgggtggaca gggggcgtcg gggtcgaata tctcctcgcc gaccggtgga
    21121 gcttcggcct cgagtacgat cactacgagc tgcggctcgg cgatcgcagc gcgatccagt
    21181 cgaacggagc ggtcgccgac tacaccgggt tcaaaggcag catcgacgcg ctctacgcgc
    21241 ggctgaaccg cacgttctga gagggccggg cgccaagcta taattcggcc atccgccgcc
    21301 cgcgctcgca cgccgggcac caccgaggcc caccttggaa cagcaggacc tttcccgtct
    21361 acggatcgac cgcgccgcgg cgcccctgcg ccggcgccgg cgcgggccct ggatcgcggc
    21421 ggccgtcgcg atcgtggcgg tgctcgcggg cttcgcgtat acgcggttcg gcgctccgtc
    21481 cgaggtcgag acggtcgccg tggcgcggat ctttccgtcg caatcgctcg ccgtcctgaa
    21541 cgcgaccggc tacgtcgtcg cccagcgcaa ggctgccgtg tcgtcgaagg ccaccggccg
    21601 cctggagtgg ctcggcgtcc tcgagggatc gcgcgtgaag aagggcgata tcatcgcgcg
    21661 cgtcgaaagc cgcgacgtgg cggccacggt cgaccaggca tcggcgaacg tcggggtggc
    21721 gcaggcgaac ctggaacagg ggctcgccga gctcgccgac gccgagagcg cgttcaagcg
    21781 ctccgaggag ctgctcgcgc agaagtacat atccccttcg gcccacgact cggcgatcgc
    21841 gcgctaccac aaggcgcgcg ccgcgatcgt gggcctgcgc gcggccatcg ccgcggcgag
    21901 cgccaaccag cgcgccgcgc aggtcgcgtt cgagcagacg ctcatccgcg cgcccttcga
    21961 cggcgtggtg ctcaccaaga gcgcgaacgt cggcgacatc gtcacgccgt tctccgccgc
    22021 cgtcgacacg aagggcgtcg tggtcaccat ggcggacatg gagacgctcg aggtcgaggc
    22081 cgacgtctcc gaggcgagcc tcggcaagat ccgcgtcgac caggcgtgcg agatccagct
    22141 cgaggccttc cccgacgtgc gcttcccggg cgtcgtgagc cgcatggtcc caacggtgga
    22201 ccgcacgaag gcgacgctgc tcgtcaaggt ccgcttcacg cagcccgacg cgcgcgtgct
    22261 gcccgacatg agcgccaagg tcgcgttcct gtcgcgcgcc gtgacgcccg aggagcgcag
    22321 cgcggtggac gcactgcagc gctccgcaat cgtcacgcgc ggcggccgca gcgtggtctt
    22381 cgtggtacgc gatggacggg tgtcggaggt tccggtggag accggccgca cgctcggcga
    22441 cctgctcgag gtgaagggcg tcgccgccgg cgaccgggtc gtgctgcgcc cggacgagca
    22501 cgtgcgcgac ggggagcggg tccgcgcgac caagtcctga cccgcatgag cgaagcgcgg
    22561 gcgccgctgg tcgagatccg caggctcgcg aaggcgtacc gccgcggcgg ccaggccgtg
    22621 cccgtcctct ccgacatcaa cctcgagatc gccgaggggg atttcctcgc gctgatgggc
    22681 ccgtcgggct ccggcaagag cactctgctc aatctcatcg ccggcatcga caagccggac
    22741 gcgggcgagc tgcgcgtcgc gggcgaggac atcacgcgtc tctcggaggc cgagctcgcc
    22801 gactggcgcg cggcgaacgt cggcttcatc ttccagttct acaacctgat gcccgtgctt
    22861 acggcgttcg agaacgtcga gctgccgctg atgctcacgc gcctcgcgcg ccgggagcgg
    22921 cgcgagcgcg tggacatgat gctgtccctg gtcgggctgg ccgagcgcgc gagccactac
    22981 ccctccgagc tgtcgggcgg gcagaagcag cgcgtcgcca tcgcgcgcgc catcgtcggc
    23041 gatccccggc tcatcgtcgc cgacgagccg acgggcgacc tcgaccgcgt ctcggccgcc
    23101 gagatcctcc acctgctgca gcggctcaac cgcgagcttg gcaagaccgt gatcatggtg
    23161 acccatgacg ccaacgccgc gagcgccgcg cgcgcggtga tccacctgga gaagggcgag
    23221 ctggtcgccg aaccgtgacc gcgcacgtcc tcaagctcat cgtcaagaac gccctgcgcc
    23281 acaagctgcg cacggcgctc accgcggcgg gcatcgtcgt cgcgatcctg tcgttcggac
    23341 tgctgcagac cgtgatcggc gcctggtacg cgggcgccaa tgccgcctcc gacgcgcgcc
    23401 tcgtgacgcg caacgccatt tcgctcgctt tccccatgcc gctcagctac gcgtcgcgca
    23461 tccgcgcggt cgacggcgtg gcggccgtga cgcacgccaa ctggttcggc ggcgtctacc
    23521 aggacccgaa gaatttcttc gtgcaattcg cggtcgacgc gcgcacctac ctcgaccttt
    23581 tcccggaatt caggctgagc gaggccgagc gcaccgcgtt cgtgcgcgac cgcaagggtg
    23641 cgatcgtcgg gcgcaagctc gccaaccagt acggcttcaa ggtcggcgac tcgatcccgc
    23701 tgcgcggcac gattttcccc ggccagtggg agttcgtcgt gcgcggcatc tacgacggcg
    23761 gcaagccgac cacggacacc tcgcagatgc tgttccagtg ggactacctg aacgagtcga
    23821 tgctgaagac tttcccgcgc cgcgcgaacc aggtcggttt ctacgtcgtg cgcatcgaga
    23881 acccggacga cgctgcggcc gtctcgcgcg cgatcgacga gcagttcaag aactcgatcg
    23941 ccgagaccgt caccgagacg gaaaaggcgt ttcagctcgg gttcgtgtcg atgtccgagg
    24001 cgatcgtggt cgcgatccgc gtcgtgtcct tcgtcgtgat cgtgatcatc ctcgccgtga
    24061 tggcgaacac gatggcgatg agcgcgcgcg agcgcctggc ggaatacgcg acgctcaagg
    24121 cgctcggctt cgggccggcc ttcctcgcga ccctgatcct gggcgagtcg gtggcgatct
    24181 gcgcggcggg tgctgccgcc gggatcgcgc tcacgttccc ggtcgcggcc gcgttctcgg
    24241 ccaagatggg cacgcttttc ccggtgttcc acgtggaggc ttcgacgctc gtcatgcagg
    24301 cggtcgcggc gctcgtggtg gggctggcgg cggcggtcgt gccttcgctg cgctcggcgc
    24361 ggatctcgat cgtgaacggc ctgcgtcacg cgggatgagg cgatggcgtc cggcatcccg
    24421 gtgtactacg tcgcgcgcaa cctgtggacc cggcgcctca cgacggggct gaccgcgggc
    24481 ggcatggcgc tggtcgtctt cgtcttcgcg gtagtcctga tgctcgacga gggtttgaag
    24541 cgcacgctgg tcaccaccgg cgagtacgac aacgtcgtcg tcacgcgccg ggcgtccgag
    24601 accgagatcc agagcctgat tcagcgggag caggtgccga tcgtcgagac gcaccccgcg
    24661 gtcgcgctcg accgcgacgg gacggcgctc gtgtcgagag agctggtggt gctgatctcg
    24721 ctcaacaagc gcaactcgga gaagccctcg aacatcgcca tccgcggtgt gtcgccgcgg
    24781 gcgctcgagc tgcggccgca ggtgaagatc gtcgaaggca ggatgttcga ggccgggaaa
    24841 tcggagatca tcgccggcag cgggatcacg cgccgcttct ccggcgcggg cgtcggcgag
    24901 cggctgcgct tcgggcagcg cgattggacc gtcgtgggcc tgttcgacgc gggcaacagc
    24961 ggcttcgact cggagatctg gggcgacgtc gagcagctcg cgcaggcgtt caggcggacc
    25021 acgtactctg ccgtgatcgt gcgcctggcc gacactcggc gcttcgacca gctcaagcgc
    25081 gacatcgagt cggacccccg gctcacgctg gaggcgaagc gcgagcaggt ctactacgcc
    25141 gaccagtcca aggcgctttc cacgttcatc agcatcctcg ggctgacgct ctcgatcatc
    25201 ttctcggtcg gggcgatgat cggggcgatg atcaccatgt acgcctcggt cgcgaaccgc
    25261 gtggccgaga tcggcacgct gcgcgcgctg ggattccggc gccgcgcgat cctcgccgcc
    25321 tttctcatgg agtcgatgct gctgggactg gtcgggggcg gcgtggggct gttcttcgcc
    25381 tcgttcatgc agctcgtgac cttttcgacc atgaacttcc agtcgtttgc cgagctcgcg
    25441 ttcggattca ccctgaacgg cgcgatcgtg gcgaagtcgg tcgccttcgc ggcgatcatg
    25501 ggcctggtcg ggggcgtgct gcccgcggct cgcgccgcga ggatgaagat cgtggactcg
    25561 ctgcgcgccg cctgagtcag tcggcgctcg cgcccgtctt cgcgatgatg ctgcgccagc
    25621 gctcgagctc cgagcgcatg agcgcaccga gctcctcggg gctgctgccc gcgggctcga
    25681 agtacaggcg gaggaacttc tcgcgcacct cgtcgctgtg gatgatcgcc cgcagctcgc
    25741 ggctcaggcg gtcgatggtg ggctggggcg tggcggcggg cgcaacgacg gcgttccaag
    25801 cgaccgacac cacgcctgga aacccggact gcgcgacgct cggatacgcc ggcagcgccg
    25861 cgctcttgcg ctcggtggag acggcgagcg ccttcaggcg ccccgactgc acgagcggca
    25921 tggccgtggc cggcacgacg aacgccgccg cgacgtcgcc ggcgacgatg gcggcgttga
    25981 cctgggggct ccccgggtaa ggcacgtgca cgaggtcgat gcccgcggcc tccttgaaca
    26041 tctccatcgt gaggtgcgag gcgctgccgt ttccgaccga cccgtagttc agcttcccag
    26101 gctgcttctt cgcgagcgcc acgagatcct tcacggaggc gacgccgagc ctgggatcga
    26161 cgacgagcag gttcgggctg gtcgcgacca gcgtcaccgg cgcgaggtcc tcgaacgggt
    26221 cgtacccgag cttcttgtag agcaccttgt tgacagcgag cggaccctgg atcgagagcc
    26281 cgacggtgta gccgtcgggt gcagcgcgcg cgaccgccgc ggtgccgacg ttgccgcccg
    26341 cgcccgggcg gttctcgacg atcaccgggt gctcgagcgc gggggcgagc ttctcggtga
    26401 ggatgcgcgc gatcaggtcg ggcgagctgc ccgccgggaa gggaacgatc acgtgcagca
    26461 cgcgcccctg gcccggtccc gagctcgccg aaggggcgga ttgcgcgtgg gcgcccagcg
    26521 gcgccagcgc gatcgcgatc gcggcgagta cgcggcgcgt acgcatcact cgagcacggc
    26581 gtagaccgtg tcgttctcga tctcgacctt gtaggtcttc agccgctcgg tgcacggcgc
    26641 tccggtcggc tcgccggtgc gcacgtcgaa ggtgccgccg tgccagctgc attcgaccgt
    26701 ctcgtcgagc aggtagccgt cctcggtaag cgacgcgccg ccgtgcgtgc acgtgtcgtc
    26761 ggtgcagaag aggctgccct cgacattgaa cagggcgatc ggcgcgcggc cctccacctc
    26821 gatcttgcgc gactcgccgg gagcgacttc cttgagcttg cagatctcga tcttcatcct
    26881 gcgattttac cgcgaaggcc ccggcgcgcg aagcgtcccg gctcgcgctc gctcaggcgc
    26941 ggtgcttgtc gaggaaggac aagagcgccc ggttgaaggc ggcggtctgc tcgagattcg
    27001 cgatatgcgc agcggacgga atgacgacca gctccgatcc ggagatgttc tggtggatca
    27061 ccttcgccac ctcgggcggc gcgcccgggt cctgctcccc cacgatgacg agcgtcggca
    27121 ccttgatcgc cttcagccgg tcggtgaaat tcagagtcgg gatcgcggcg cagcagccgg
    27181 cgtagccggc gaccggggtc gagcggatca tcgcggccag cctcgcggcc gtctccgggt
    27241 gcgacttgcg gtagggctcc gtgagccagc gctgcagcgt cggctcgacc acgccttcca
    27301 tgcctttctc gcgcgcggtc ttgacgcgcg cctcccagac cggcttcgcc tccggcggat
    27361 tgcggctcgt ggtatcggcg aggaccagcg tctcgaagac gcccgggaac ttgagcgcga
    27421 aggcctgccc gatcattccg ccgatcgaga gcccgaccca gtggcacgac ttgattccga
    27481 gcgcctcgag cagcgcgtgc gcgtcgtacg cgagcatgtc gaaggtgtag tcgccggcgg
    27541 gtgcgtcggt gccgccgtgg ccgcgcaggt cgtatcgcag caccttgtag cgctttgaga
    27601 gcgcggcggc ctgctcgtcc cacatcgaca ggttgcacgc gagcgagtgg ctcatgacga
    27661 cccacgggcc ctcgccctcg acgctgtaat tgatctggat gccgttggcc ttcactttca
    27721 tggggatcct tccggtgcgg tatgcggggt ggcgggaaat tctactccgc ggccaggctc
    27781 agatcagggc gcgcgcgctc agctcttcct tgaggtacgc gtagtagacg ggcgcagcga
    27841 ccagccccgc gagcccgaac gcggactcca tgaccagcat cgcgagcagg agctcccacg
    27901 cgctcgcgcg gatctgcgag ccgacgatgc gcgcgttgag gaagtactcg agcttgtgga
    27961 tgacgacgag gaaagcgagc gacgcgatcg caaccgcgag ggacgccgac aggctcacga
    28021 cgacgatgat cgtattggag agcaggttgc cgatcacggg cagcagcccg gcgacgaagg
    28081 tgatcaggat cagcgacttc gcgaagggca ggtgcacgcc gagcagcggc agcgcgacgc
    28141 ccaggtagat cgccgtgaac gcggtgttga tggcggcgat gcgcacttgc gcgaacacga
    28201 tgcgccggaa cccgtcgccg agcagggcgg cgcgctgccc gagcgcacgc gcgagcggcg
    28261 caccgtggcg cgacggctgc gcctcgcgca gggaaaccat cgcgccgatg atcatgccga
    28321 tcaggaggtg ggcgctgagg cggcccgcct ccttgccgag cagctgcacc tcgcccgcgt
    28381 gctcgcgcag ccagtcggtg agcgcctggc gcagctcctc ggcgtccgtg ggcaagtact
    28441 gcagcaccca gccggggagg tgcgagcgcg atccgtcgat gatctccgcc atcttctgca
    28501 ggagcgccgt caggctcccg gcgtcgctgc ggaagaagct gaccgcgccg aagagcgcga
    28561 gcgacagcgc cgcgacgatc gcggtcgcca ggatcgcgac cgcggccacc ttgccgcgct
    28621 tgccgagccg caggcgcggc gcgatgacgt gcaccagctc gaatacgagc aggccggcga
    28681 gcagggccgg gagcagatgg agcagcagcg tgagcagcag gagcagcccg gccaagatcc
    28741 aggcggcgac ctcgaggcgg ggcgaggaag gggtcggcat gtgggggcac aggctagcac
    28801 acgctctggg ataatggtcg cgcctcattc tcgctcgagc gcgacccatg cccgactcgg
    28861 ttctcgtcac cggcggcgcc ggcttcatcg gctcgcacgc ctgcgtcgag ctcgcgctcg
    28921 cgggctacga cgtgaccgtc gtcgacaacc tcgcgaacag tcgccgctcg gttatcggcc
    28981 gcgtgtcccg catcgccggg cgcccgatcc ggttcgccga aggcgacgtc cgggacaagg
    29041 acttcctccg agcggtattc gcggcgcgcc gtttctcggc ggtcgtgcat ttcgcgggcc
    29101 tgaaagccgt gggcgagtcg gtggccgagc cgcttcggta ctgggagcac aacgtcggag
    29161 gaacgctcac gctgctcgcc gcgatggccg agcatggcgt gcggcggatc gttttcagct
    29221 cgtcggcgac ggtctacgga cagagcgccg cgatgccgct cgccgaagac gcgccgctcg
    29281 ggccgaccaa cccgtacggt aggaccaagc tcgccatcga gcagatcctc ggcgatgtcg
    29341 ccagggccga cgcgcagtgg agggcggtgc tgctgcggta tttcaatccc gtgggcgccc
    29401 acgcgggcgg cacgatcggc gaggatcccg acggcgtgcc gaacaatctc tttccctatg
    29461 tcgccggcgt ggccgccggc cgtttcgagc gcgttcccgt cttcggcaac gactatccga
    29521 cgccggacgg tacgggcgtg cgcgactaca tccacgtcat ggacctggcg gccggccacg
    29581 tcgcagcgct tcgggcgctc gacgcgctgc ccgcagtgac tgcggtgaac ctcggcaccg
    29641 ggcgcggcta cagtgtcctc gaggcgatcg ccgccttcga gcgcgcctcg ggccgcaaga
    29701 ttccattccg ggtcgagccg cgacgcccgg gcgacgtcgc gatgagctac gccgacccgt
    29761 cgctcgccgc ccgcctgctc gcctggaagg ccgttcgcgg gctggacgat atgtgcgccg
    29821 acgcctggcg ctggcagcag tggcgagccg cgcacgaggc gcagctctag aggttctcta
    29881 ttccttcacg cgcgcgatcc ggactacttc ctggatgcgg cgcaacgacc gcatcacgcg
    29941 cgcgaggtgc atgcggttcg cgacctgcag cgtgaaatgc atggtggtgt agaggctgcg
    30001 ctcctcgtcc atgctgacgt tgtcgatgtt cgagccggct tcggcgatgg ccgcggccac
    30061 cttggcgagc acgcccggct ggttgacgac gatgacgcgc acggccacgt cgaagagctt
    30121 gtcggtgtgc ggcgcccact ggacgtcgag ccacttgtca gggtcgccgc gcgccttcgc
    30181 cgcggtcgtg cagtcgtgcg tgtgcacgac cagtccctga cctttcttga tgaagccgat
    30241 gatgggatcg cccgggaccg gccggcagca ctttgcgaac tgcacggcca tgccctcgga
    30301 gccatggatg acgatggacc cggcggcgaa cttcgcctcg gacgggtgtg cgtcggacag
    30361 cgccagcagc ttccgggcga cgatcgcggc gagacgcttg ccgagcccga tatcggcgac
    30421 gatctcctgg cgcgagcggg cgccgagctc gcgcacgagc ttctcccagc gctcgggcgt
    30481 cggctcggcg tggggtgtgc cgagagcgcg caccgcctgc gcgagcatgc gctcgccgag
    30541 catcgctgcc tcctcgatct gcatggtctt caggaagtgc cggatctgga agcgtgcctt
    30601 ggcggtcttg acgtaggtga gccaggccgg gttcgggtgc gcgtgggctg cggtgatgat
    30661 ctcgacgcgg tcgccgttct tgagctcggt ccgcagcggt gcgagctcgt agttgatgcg
    30721 cgccgcgacg cagcggttgc cgatgtcggt gtgcaccatg taggcgaagt cgaccgcggt
    30781 cgcgccgcgc ggaagcgcca tgatcttgcc cttcggggtg aacacgtaga cctcgtcggg
    30841 gaagaggtcg accttgatgt gctcgaggaa ctccgcggcg tcgggcgcgg cgtggtcgat
    30901 gtcgagcagc gactgcagcc acttgtgcgt gcgcttctgc acctcgttga tctcggcgtc
    30961 ggtgctcttg tagagccagt gcgaggcgac gccggtctcg gcgatgcggt gcatggcttg
    31021 cgtgcgcacc tggatctcga ccggcgtccc gtaggggccg atcagcgtcg tgtgcagcga
    31081 ctggtagccg ttgaatttcg gtatcgcgat gtagtccttg aatttccccg ggaccggctt
    31141 gtacaggcca tggagcgcac cgagcgcgag gtagcacgag ggcacgtcct tcacgatcac
    31201 gcggaagccg tagatatcga gcacctggga gaacgagagg tgcttctcca gcatcttctt
    31261 gtagatgccg tagatgtgct tctcgcgacc ggtgacgttc gcctcgatcc cggccgcggg
    31321 caggcgctcc ttgaccgcct ccaggatctt gttcacgacc tggcggcggt tgccacgcgc
    31381 ggccttcacc gccttggcga gcgtcttgta gcgcagcggg tagagatgcg agaacgcgag
    31441 ctcctgcatc tcgcggtaca ggctgtcgag accgaggcgg ttggcgatgg gcgcgtagat
    31501 ctcgagcgtc tcgcgcgcga cgcgtcggcg cttctgcgtg tcgaccgcgt cgagcgtgcg
    31561 catgttgtgc aggcggtcgg cgagcttgat cagcatgacg cgcacgtcgc gcgccatggc
    31621 gagcagcatc ttcctgaagt tctcggcctg cgcgtcctgc tgcgactgga actcgatgcg
    31681 gtccagcttg gagaggccgt cgaccagctc cgccaccggc ttgccgaaac gctggctgat
    31741 ctcgcccttg gagacctccg tgtcctccat gacgtcgtgc aggagcgcgg ccatcaccgc
    31801 ctgggcgtcg agctgccagt cggcgatgat ctcggcgacc gcgatcgggt gcgagatgta
    31861 agggtggccg ctcttcctga actggccctc gtgggccgcg ctcgagaact ggtacgcctc
    31921 ctcgatgcgc gagacgtcgc tcggcttcag gtagccgagg tgctgccgga gatcggtgat
    31981 ggcgacggcg gccatggcac tactttaccc cttggccgca ggggtcggcc ggcgttcagg
    32041 cggccgtcag gcggtattga ccttgctgag gtactcgatg ccgaccttgc cggtcgcgac
    32101 ctcgcgcagc gcgatgacgg tgggcttgtc gcggttcgcc tcgatgagcg gcgtgccgcc
    32161 ctgggcgagc tggcgcgcgc ggtaggtcgc cgccagcgtg agctggaagc ggttggggat
    32221 cctcttcagg caatcctcga cggtgatccg tgccatgtga aacctctcgt caatccggcc
    32281 gctggaacgc ttcggggtgt cgcgcgcgct ggcgctcggc ggtcagtcgg gaggcgcgca
    32341 cgatcgcgct caggtcccgc ttcgcctctt cgaagtcctt gttgataata acatattcga
    32401 actcggctgc gtgcgccatc tcctcgcggg cgttggcgag ccggcgcgcg atcgcttcct
    32461 cgtcgtcgtg accgcgcttg cgcagccggc gctcgagctc ggcaagcgcc ggggaaggcg
    32521 gcaggacgaa caccgagacc gcctcgggga aggccttgcg cacctggcgc gctccctgcc
    32581 agtcgatctc caggatgatg tcgaggccgc gcccgcgcgc gtcggcgatc cactttcgcg
    32641 acgtgccgta gcggttgccg tgcacctggg cgctctcgag gaactcgccg cgctcgagca
    32701 tcgccgcgaa ggcgccggcg tcgacgaagt gatactcgcg gccttcctgc tcgctcggcc
    32761 gcggggcccg ggtcgtgtac gagaccgaga gcgcgagatt acggtcctgc gcgagcagcg
    32821 cgctcacgag cgaggatttt ccggcccccg acggggccga gatgatgaag agatcgccgc
    32881 tcatcgccta gcctactcga tgttctggat ctgctcgcgc atctgctcga tgagcagctt
    32941 gaactcgaga ctcgcgtcgg acacttcctt cgagaccgat ttcgaggcga gcgtgttggc
    33001 ctcgcggttc agctcctgca tcaggaagtc gaggcgcttg ccgaccacgc cacccgcgtt
    33061 gacgacgcgc cgcacttcgt gcaggtgcgc ggcgagccgc gtgagctcct cggcgacgtc
    33121 gattttcacg ccgaagagcg cgatctcctg gcggatgcgc tcctcgtcgg aggcgccgag
    33181 cgcttcgcgc aggcggcccg cgaggcgctc ctcgtacgcc gccaccgcct cgggcaggag
    33241 cggctggacc tcggcgagca ggcgctccat gcgctcgatg cgctcgagca gcatgccggc
    33301 gagcttgcca ccctcgcgcg cccgggtcgc ggcgaactcc gcgagcagct gctcgaccag
    33361 cgcgagcgcc gtgtcgcgaa ccagcgcgct gcgatcgtcg ccgagcacgc cgggccagtg
    33421 cagcacctcg ccgacacgca gcggagccgc ttcggggatc gcctcgcgca cccggcgcga
    33481 ggcttcggcg agcgccgaga gcagcgtctc gttcaacgcg aggctcggcg cgccgccggc
    33541 ggcggcggtc acggccacgc ggcactcgac cttgccccgg ctcacccggg cggcgatcat
    33601 ctcgcgcagg gcgggctcga gcgcgcgcat ctcgtcgggc agccggaact ggatgtcgag
    33661 atagcggctg ttgacgctct tcagctcggc cgcgagcgag cactgcggca ggtcgcgcgc
    33721 cgacgaggca tagccggtca tgctgtggat catgactggg gacggggaat cgtgatcaat
    33781 tgggcgacgg ccttgctaca atgaccggcg cccgcacgca aagcgcctat gatagcgggt
    33841 cgccccagcc ggcggcgaac taagtgacga cggaaccggg gggagacgtc gcctcggcaa
    33901 accagggatg cctcaacaag ccaaccagcc gctgccgaac ggttacctgc tccagaacta
    33961 tcgcatcgcc agcgtgctct cgtgcggggg cttctcgatc gtctacctgg cctacgacga
    34021 gaacgaccag ccggtcgcga tcaaggaata cctgccgagc cagctcgcgc tgaggaagga
    34081 gggcgacgcg ctgccgtcca tctcggacga caacctcgcg accttccgct acgggatgaa
    34141 gtgcttcttc gaggaaggcc gctcgctcgc gcgcctgtcg cacccgaacg tggtgcgagt
    34201 gctgaacttc ttccgcgcga acgagacggt gtacatggtg atgcgctacg agcgcggccg
    34261 cacgctccag gagcatatcc aggcgcgccg gggctcgatc aaggagaact tcatccggca
    34321 cgtgttcgcg ctgctcctga acgggctgcg cgaggtgcac tccaacaagc tgctgcacct
    34381 tgacatcaag ccggcgaaca tctacatccg gaacgacggc agccccgtcc tcatcgattt
    34441 cggggccgcg cgccagacgc tcgcagagga aggcttgaag ctcaacccca tgtacacgcc
    34501 gggcttcgcg ccgcccgagc agtaccgcaa ccgcgagctg ctcgggccct ggagcgacgt
    34561 gtacgccatc ggcgcgagca tgttcgcgtg cctcgccggc gtcgccccgc aggccgcgga
    34621 ccagcgcctg gagaaggacc gctatgtgtc ggcgacgaag acctggcccg gcaagtactc
    34681 gcgccacctg ctcgagacga tcgactggtg cctggagctc gaccacctca accggccgca
    34741 gagcgtgttc gcgctgcaga aggtgctgct gcgcgagaag gaacccgagg tcaggcgcaa
    34801 gcgctcgctc gtcgaccacg tgcgcggcgc gttcctgaag atcgcccggc gatgaagttc
    34861 tccatctacc aggaaagccg caaaggcggg cgcaaggcga accaggaccg catcggctac
    34921 agctatacgc gcgattcgct gctcatgatg ctcgccgacg gcatgggcgg gcacctgcac
    34981 ggggaggtcg catcgcagat cgcggtgcag ttcctcaccg aggcgttcca gcgcgaggcg
    35041 cggcctcgac tcgccgatcc cctgcagttc ctgtcgaaga gcatcgtcaa cgcgcaccac
    35101 gcgctggtcg actacgccaa ggtgcggcag ctcaccgaca cgccgcgcac gacctgcgtg
    35161 gcttgtgtcg tgcaggaagg catcgcctac tgggcgcacg tcggcgactc gcgcctgtac
    35221 cacctgcgcg agggcaagat ccaggcgcag acgcgcgacc actcgcgcgt gcagatgctg
    35281 gtcgactccg gccgcatccg cgaggaggcg gtctccgcgc accccgaccg caacaagatc
    35341 ttcaactgcc tgggcgcgca cactccgccg caggtcgagc tctcgcacaa gacggtgctg
    35401 cgcaccggcg acacgctgct tctgtgcagc gacggcttgt gggggccgct ctcgggcaag
    35461 atcatcgccg cggccctgct caccggcgac gtcctgcatg ccgtgcccga gctgctgacg
    35521 caggccgagc agcgcgccgg caaggatgcg gacaacctct ccgtcgtcgc gatgaactgg
    35581 accgacagcg tccaggtgca atcgcccggc gaggtgtcga cgcagacgct cgagctcgac
    35641 gccgtgatca cccaggtcga ggacttcaag aaggaagatc acagcgatct caccgacgag
    35701 gagatcgagc gcgcgatcgg ggaaatccgc gacgccatcc gcaagcactc accgcagaag
    35761 ccatgagcac gcgtccatcc caacgccgtc ccgacgagct gcgcgccgtg tcgctgcgac
    35821 gcgcctacac gcgccacgcc gagggctcgg tcctcgtcga gttcggcgac acgcgcgtgc
    35881 tgtgcaccgc gagcgtcgag gagaaggtcc cccccttcct ccgcgaccgc ggacagggct
    35941 gggtcaccgc cgaatacggc atgctgccgc gctcgacgca cacgcgcacc gaccgcgagg
    36001 cggcgcgcgg ccggcagtcg ggccgcacgc tcgagatcca gcgattgatc ggccgcagcc
    36061 tgcgcgcggt gaccgacctc gagctcctcg gcgagcgcac gatcacggtc gattgcgacg
    36121 tgatccaggc cgacggcggc acgcgcaccg ccgcgatcac gggtggctgc gtcgcgctgt
    36181 acgatgcgct gtcgacgctg gagcagcgcg gcctggtcaa ggcccatccg gcgcgcgatt
    36241 tcgtcgccgc ggtgtcggtc ggcgtctacg acggcgtgcc ggtgctcgat ctcgactacg
    36301 ccgaggattc ggcctgcgac accgacatga acgtcgtgat gaccggctcg ggcggcttcg
    36361 tcgaggtgca gggcaccgcc gagggcgcgc ccttcacgcg cgcgcagctc gatgcgctgc
    36421 tcgagctcgc gcgcgccggc atcgcccagc tcgtggcgcg gcagaagcag gcgctgggcg
    36481 tctgagcggg cggtcgccgc cgccgcgata aaatgtcgcg gtgaagaagc tcgtcctggc
    36541 ctcgtccaac cccggcaagc tgcgcgagat cgaggcgctg ctggcgccgc tcggcgtcga
    36601 agtagtcccg caatcgcggc tcggcatcgg cgacgccgag gagccgcacg cg
//
LOCUS       DATDMW010000252         2937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1072449, whole genome shotgun sequence.
ACCESSION   DATDMW010000252 DATDMW010000000
VERSION     DATDMW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072449"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VI319_11050"
     CDS             complement(<1..568)
                     /locus_tag="VI319_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6864425.1"
                     /translation="MTVVITAFERSPDGGQGLARDTRVRWALEEVGQPYEVRGVSFRA
                     LKEPAHRALHPFGQIPTYEEDGLVLFETGAIVLHIAERHAGLLPPDPDARARAIAWMF
                     AAVNTVEPPILELANARLLESDKPWSRERLPLVEDRVRDRLRPLSDRLGDAHWLDGAF
                     SAGDLMMVSVLLRLKPSGMLTGFPRLSAY"
     gene            complement(565..>2937)
                     /gene="mfd"
                     /locus_tag="VI319_11055"
     CDS             complement(565..>2937)
                     /gene="mfd"
                     /locus_tag="VI319_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY6864426.1"
                     /translation="PDLAVERRAADPLHRLKDFLAGFSGRTLVLAESPGRRETITQYL
                     AEYGLRPEGVARFADFANTDIRLGLAVAPLAAGFALPGERLALVTESELYAGTARSRL
                     AREKRAVTTVEGMLRDLSELRPGDPVVHVQHGIGRYRGLADLDVGDGKAEFLVLEYAD
                     GAKLYVPVSQLELIGRYSGGPPEQAPLHKLGGDEWDKAKKKAARAVRDTAAELLDLYA
                     KRAMREGHAFKLDQHDYEAFAEGFGFQETPDQAGAIQAVIANMREGKPMDRLVCGDVG
                     FGKTEVALRAAFVAVADRRQVAVLTPTTLLAEQHFNTFSDRFADWPVKIGELSRFRTG
                     KETAAVLEGIAKGDIDIAIGTHKLLQKDVKFKRLGLVIVDEEHRFGVRQKERLKALRA
                     EVDVLTLTATPIPRTLAMSLESLRDLSVIATAPERRLAIRTFVAPWSPSLIREASLRE
                     LKRGGQIYFLHNDVATIESRRERLEKLLPEARIAVAHGQMRERELERVMRDFLQQRTN
                     LLLCSTIIETGIDIPSANTIIIERADKFGLAQLHQLRGRVGRSHHQAYAYLMTPEEGL
                     TAAAKKRLEAIQMMEQLGSGFYLAMQDLEIRGAGEVLGERQSGEMQEIGFNLYTDMLN
                     HAVRSLKAGREPSLDQPFDVTTEVNLHLPALLPSDYCPDVHERLVLYKRLANADSLDA
                     LEKMTEELVDRFGDLPGAARSLLESHKLRILGKPLGVARMDATSETIQIQFTPHPPID
                     PKKVLVLVQKRKGWKLAGPTKLRVERVTGTLAERASAARDVLESLAQAAA"
BASE COUNT          409 a         1043 c         1028 g          457 t
ORIGIN      
        1 cataggcgga gagcctcggg aacccggtca gcatgcccga gggcttcagc cggagcagca
       61 ccgacaccat catgaggtcg cccgcgctga acgcgccgtc gagccagtgg gcatcgccca
      121 ggcgatcgga aagcggacgc agccggtcgc gcacgcgatc ctcgacgagg ggcaggcgct
      181 cccggctcca gggcttgtcg ctctcgagga gcctcgcgtt cgcgagctcg aggatcggcg
      241 gctccaccgt gttgaccgcg gcgaacatcc acgcgatggc gcgcgcgcgg gcatcgggat
      301 ccggcggcag cagtcccgcg tggcgctcgg cgatatggag cacgatcgcc ccggtctcga
      361 acaggacgag accgtcttcc tcgtaggtcg ggatctggcc gaagggatga agcgcccggt
      421 gcgcgggctc cttcagcgcg cggaacgaaa ccccgcgaac ctcgtacggc tggccgactt
      481 cctcgagcgc ccagcgaacg cgcgtatcgc gcgccagccc ctggccgccg tcgggcgagc
      541 gctcgaaggc ggtgatgacg acggtcacgc cgccgcctgc gccaggctct cgagcacgtc
      601 gcgcgcggcg ctcgcgcgct cggcgagggt gccggtcact ctttccacgc gcagcttcgt
      661 cggccccgcg agcttccagc ccttgcgctt ctgcaccagg accagcacct tcttcgggtc
      721 gatcggcggg tgcggcgtga actggatctg gatcgtctcg ctggtcgcgt ccatgcgcgc
      781 gacgcccagg ggtttcccga ggatgcgcag cttgtgcgat tcgagcagcg agcgcgcggc
      841 gcccgggagg tcgccgaagc ggtcgaccag ctcctcggtc atcttctcca gcgcgtcgag
      901 ggagtcggcg ttggcgagcc tcttgtagag aaccaggcgc tcgtgcacgt cggggcagta
      961 gtcggacggg agcagggcgg ggaggtggag gttcacctcg gtcgtgacgt cgaacggctg
     1021 atcgaggctc ggctcgcggc ccgccttcag cgaacgcacg gcgtggttca gcatgtccgt
     1081 atacaggttg aagccgatct cctgcatctc gcccgactgg cgctcgccca gcacctcgcc
     1141 cgcgccgcgg atctcgaggt cctgcatcgc gaggtagaag cccgagccga gctgctccat
     1201 catctggatc gcctccagcc gcttcttcgc cgcggcggtc aggccctcct cgggcgtcat
     1261 caggtaggcg tacgcctggt ggtgcgagcg gccgacgcgg ccgcgcagct ggtgcagctg
     1321 cgccaggccg aacttgtcgg cgcgctcgat gatgatcgtg ttcgcgctcg ggatgtcgat
     1381 cccggtctcg atgatggtcg agcacagcag caggttcgtc cgctgctgca ggaagtcgcg
     1441 catcacgcgc tcgagctcgc gctcgcgcat ctgcccgtgc gcgaccgcga tgcgcgcctc
     1501 gggcagcagc ttctccaggc gctcgcgccg agactcgatg gtcgcgacgt cgttgtgcag
     1561 gaagtagatc tgcccgccgc gcttcagctc gcgcaagctc gcctcgcgga tcagcgacgg
     1621 cgaccacggc gcgacgaacg tgcggatcgc gagccgccgc tcgggcgcgg tcgcgatcac
     1681 cgacaggtcg cgcaggctct cgagcgacat cgcgagcgtg cgcgggatcg gcgtcgcggt
     1741 gagcgtcagc acgtccacct cggccctcag cgccttcagc cgctccttct gccgcacccc
     1801 gaaccggtgc tcctcgtcga cgatcaccag gccgaggcgc ttgaacttca cgtccttctg
     1861 cagcagcttg tgcgtgccga tcgcgatgtc gatatcgccc ttggcgattc cttcgaggac
     1921 ggcggcggtc tcttttcccg tccggaacct ggagagctcg ccgatcttca ccggccagtc
     1981 ggcgaagcgg tcggagaacg tgttgaagtg ctgctccgcg agcagcgtgg tcggcgtcag
     2041 caccgcgacc tggcgccggt cggccaccgc gacgaacgcc gcgcgcaagg cgacctcggt
     2101 cttgccgaag ccgacgtcgc cgcacacgag gcggtccatc ggcttgccct cgcgcatgtt
     2161 cgcgatcacc gcctggatcg cgcccgcctg gtcgggcgtc tcctggaagc cgaagccctc
     2221 ggcgaacgcc tcgtagtcgt gctggtcgag cttgaacgcg tggccctcgc gcatcgcgcg
     2281 cttcgcgtag aggtcgagca gctccgcggc cgtgtcgcgc accgcgcgcg ccgccttctt
     2341 cttcgccttg tcccattcgt cgccgccgag cttgtggagc ggggcctgct cgggcggccc
     2401 gccggagtag cggccgatca gctcgagctg cgacaccggg acgtagagct tcgcgccgtc
     2461 ggcgtactcg agcaccagga actcggcctt gccgtcgccg acgtcgaggt cggcgagacc
     2521 gcggtagcgg ccgatgccgt gctgcacgtg caccaccgga tccccgggac gaagctccga
     2581 gagatcgcgc agcatcccct cgacggtggt gacggcgcgc ttctcgcgcg cgaggcggct
     2641 cctcgccgtg ccggcgtaga gctccgactc ggtgacgagc gccaggcgct cgcccggcag
     2701 cgcgaacccg gccgcgagcg gcgcgaccgc gaggcccagc cggatgtcgg tattcgcgaa
     2761 gtcggcgaat ctcgcgactc cctccggccg cagcccgtac tcggcgaggt actgcgtgat
     2821 cgtctcgcgc cgcccgggcg actcggcgag caccagcgtg cggccggaaa acccggcgag
     2881 gaagtccttg agcctgtgca gcggatcggc cgcgcggcgc tcgaccgcga gatcggg
//
LOCUS       DATDMW010000253         5871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_203033, whole genome shotgun sequence.
ACCESSION   DATDMW010000253 DATDMW010000000
VERSION     DATDMW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203033"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(437..1507)
                     /gene="hemE"
                     /locus_tag="VI319_11060"
     CDS             complement(437..1507)
                     /gene="hemE"
                     /locus_tag="VI319_11060"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY6864427.1"
                     /translation="MARPANDTFLRALRREPVPYTPVWLMRQAGRYLPEYNATRARAG
                     SFLALCKTPALAAEVTLQPLARFPLDAAILFSDILTIPDAMGLGLYFAEGEGPRFERP
                     VRDAAAAAKLAPPDPGAELRYVGDAVREIVRALGGAVPLIGFAGSPWTLACYMVEGGA
                     SDDFRTVKSMLYGEPRLLHRILEVNASAVAALLAAQIEAGAHAVMIFDTWGGSLTGPA
                     YREFSLAYIERVLGAVPRSRDGVAIPRIVFTKGGGAWLEPIAASGCDCVGLDWTVDLG
                     AARRRIGDRVALQGNLDPTALFAPADAIRREVAALLASYGAGSGHVFNLGHGISQHTP
                     PENVAELVRAVHELSAPYHSGR"
     gene            complement(1507..1971)
                     /locus_tag="VI319_11065"
     CDS             complement(1507..1971)
                     /locus_tag="VI319_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY6864428.1"
                     /translation="MRHWLMKSEPDEFGIDDLAKSPRQTAPWTGVRNFTARNFMRFEM
                     QVGDPILFWHSSTDPIGIAGIAEVASAPHVDETQFDPKSDYYDERSKRDKPRWWCVDV
                     KFVRKTSFVTMDELRKHKALAGMIVLRRGNRLSITPVEPAEWGYIEKKLLGG"
     gene            complement(2044..2224)
                     /gene="ssrS"
                     /locus_tag="VI319_11070"
     ncRNA           complement(2044..2224)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VI319_11070"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2266..2583)
                     /locus_tag="VI319_11075"
     CDS             complement(2266..2583)
                     /locus_tag="VI319_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY6864429.1"
                     /translation="MSAKPKTLDVTIMGRAYKVACPDHERDALLAAVGYLDRKMTEIK
                     NTGKVGSAERIAVMAALNIAHELLTARNSSSGGFDTEGLKRRMAAMEATLDQALAPQE
                     RLI"
     gene            complement(2591..2782)
                     /gene="zapB"
                     /locus_tag="VI319_11080"
     CDS             complement(2591..2782)
                     /gene="zapB"
                     /locus_tag="VI319_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HEY6864430.1"
                     /translation="MDAELETLEQKIRQAAQLCQKLRDENRDLRQKLAALQGDKKQLS
                     EKIDGARVRLENILKQIPE"
     regulatory      2887..3134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3184..5169
                     /locus_tag="VI319_11085"
     CDS             3184..5169
                     /locus_tag="VI319_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6864431.1"
                     /translation="MKRTIVAASCLAAAFAASTVHAQEDAVVVTAQRFPERRLDAPVG
                     MTVITAEEIARDTARTVPEVLSHLGGLQVRNSSGTPDQQIDLRGFGVTGDQNTLIMLD
                     GVRLNEAELSSAKLSAIPLQSIERIEILRGSGAVLYGDNAVGGTINIVTRGPKPGRTG
                     DVLVGAGSYGTYDARASTNVATGRSGITASAEHLESDNYRANNRLRQDSMTGDWRLGD
                     ERGNVGLKFGADTQRLQLPGALSEAQIATDPRAASTPNNWSAREGAFAILSLARPLGN
                     ADFGADLAYREKESTAFFAGTGGSPATYQDTRTRNLGFSPRVRAPFRLGGIENVLVAG
                     FDWSGWDFDRRFASSVDALGGPDFGTGGSQQSAGTYVQYSGRLAQGTKLTAGAREQRV
                     VDRQVPLGFPGPEQRVVHTMDAGEVGLEQALAERFTLFGKYGKSFRIATVDENGFTLT
                     GEPLKPQTARSKEAGVEYRRSDTRLRLSAYRIDLDNEIHFNPLAAPPFGANVNLPPTR
                     RQGAELFASGRLVPALEVSGNFIAQSARFRTGDFGGADLAGNQVPLVPRRLATLRAAW
                     QPAPGTVATVSTRYVGRQRYDNDQANLFREMPSYSVTDLKIAHRRGRATWGLALNNVF
                     GKRYYSYAIVDSPTAPTTFNAYPEAGRSVMGTLELAL"
     gene            5166..>5871
                     /locus_tag="VI319_11090"
     CDS             5166..>5871
                     /locus_tag="VI319_11090"
                     /inference="COORDINATES: protein motif:HMM:NF002204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEY6864432.1"
                     /translation="MSASPRLVVAAPHSGSGKTTFTLGLLAALVRRGLAVQPFKAGPD
                     FIDPSHHTAVCGRVSRNLDTWMCDDATVAAIFGRGARGADVSVVEGVMGLYDGFGPDE
                     QRGSTAHLAKLLAAPVVLVIDAQRMATSAAAVALGLREFDRGVAIAGVVFNNVGSEGH
                     YEWLRKALEARTGLESLGYLLHDPALRIDERHLGLVPAAERALAKEALARSADRLERH
                     VALDRILAAARAPPRPP"
BASE COUNT          912 a         2003 c         2036 g          920 t
ORIGIN      
        1 ggaccaccgc acgcgtgccg atgtcgtcgc gcgtcgcgtc gtcggcgcgg taatcgaaga
       61 gcttcggcag cgggacgtcg agcgcgacgc gcgcgacggt tgccgcgctc aggttgaggc
      121 gcgaatcgtc actcaagtgg agtctgcgta actcgggata agcagcgagc ccgtatggtg
      181 attttcgggt tcttcacaga atttgtggat aacggtgtgc gtaaccgtgt cacacaaacg
      241 taacctgcgt tggcgaaaag gattttttcc gctgcctaaa atggcggcgt gccataaatg
      301 gtaatacaaa caaatagata tatgtctctc cctggggaca ccccggaacg ccgcgcctgg
      361 cgcggggttt cagggggtcg tgtgtataag tccgaattgc ggttcccgcc gcgtctaatt
      421 ggcgggacgc gcattgtcag cgtcccgaat ggtatggggc gctcaattcg tgcaccgcac
      481 gcaccagctc ggcgacgttt tcgggcggcg tgtgctgcga aatcccgtgc cccaggttga
      541 agacgtgtcc gctgcctgcg ccgtacgagg cgagcagcgc cgcgacctcg cgccggatcg
      601 cgtccgcggg cgcgaacagc gccgtcggat ccagattgcc ctgcagcgcg acgcgatcgc
      661 cgatgcggcg ccgcgccgcg ccgaggtcga ccgtccagtc caggccgacg cagtcgcagc
      721 cgctcgcggc gatcggctcg agccaggcgc cgccgccttt ggtgaagacg atgcgcggga
      781 tcgcgacgcc gtcgcgactg cgcgggactg cgccgagcac gcgctcgatg tacgcgagcg
      841 agaactcgcg gtacgcgggg ccggtgaggc tcccgcccca ggtgtcgaag atcatgaccg
      901 catgcgcgcc ggcctcgatc tgcgccgcga gcagcgcagc gaccgcgctc gcgttcacct
      961 cgaggatgcg gtgcaggagg cgcggctcgc cgtagagcat cgatttgacg gtgcggaagt
     1021 cgtcgctcgc gccgccttcc accatgtagc aggcgagcgt ccaggggctg cccgcgaacc
     1081 cgatcagcgg caccgcgccg ccgagcgcgc gcacgatctc gcgcaccgcg tcgccgacgt
     1141 agcgcagctc cgctcccgga tcgggaggtg cgagcttcgc cgccgccgcg gcgtcgcgca
     1201 ccgggcgctc gaagcgcggg ccttcgccct cggcaaagta cagtccgagg cccatcgcgt
     1261 ccgggatggt gaggatgtcg gagaacagga tcgccgcgtc gagcgggaag cgcgcgagcg
     1321 gctgcagcgt gacctcggcc gcgagcgcgg gcgtcttgca cagcgcgagg aagctgccgg
     1381 cgcgggcgcg cgtcgcgttg tactcgggca ggtagcgccc ggcctggcgc atcagccata
     1441 ccggcgtgta cggcacgggc tcgcggcgca gcgcgcgcag gaatgtgtcg ttcgcgggcc
     1501 tggccatcag ccgccgagga gcttcttctc gatgtagccc cactcggcgg gctcaaccgg
     1561 cgtgatcgag aggcggttgc cgcgccggag cacgatcatg ccggcgagcg ccttgtgctt
     1621 gcgcagctcg tccatcgtga cgaaggacgt cttccgcacg aacttgacgt cgacgcacca
     1681 ccagcgcggc ttgtcgcgct tcgagcgctc gtcgtagtag tcgctcttcg ggtcgaactg
     1741 cgtctcgtcg acgtgcgggg cggaggcgac ctcggcgatg ccggcgatgc cgatcggatc
     1801 ggtgctcgaa tgccagaaca agatcggatc cccgacctgc atctcgaagc gcatgaagtt
     1861 gcgcgcggtg aagttgcgca cgccggtcca cggcgccgtc tggcgcggcg atttcgcgag
     1921 gtcgtcgatg ccgaactcgt cgggctccga cttcatcagc cagtggcgca tgggagcagg
     1981 gaggatagct gaaataaaaa acgcaggcct ggcctgcgtt tttcaattgc ttcttcctat
     2041 ggggtgcccc ccgccgatgc cgctggaccc tcttcctgaa ccttgcggtc caagatggtc
     2101 gcttcccggt cacatcaggt acgtccgacg ggggacgcgc acaacagtga cgctagagtc
     2161 cgcaaccaaa ttcaagctgt tggttcaaga atttttcagg ccttgacgca catcgcaggg
     2221 gacaattctt cggcggcgcg atcggactgc cttgccgctg cttcgctaga tcaaccgctc
     2281 ctgcggagcg agggcctgat cgagcgtcgc ctccattgcg gccattctac gcttcaagcc
     2341 ttcggtgtca aacccgcccg aggacgagtt tctcgcggtg aggagctcgt gcgcgatgtt
     2401 gagcgctgcc atgactgcga ttctctccgc gctcccgact ttgcccgtgt ttttgatctc
     2461 ggtcatcttg cggtccagat agcctacggc cgcgagcagc gcgtcgcgct cgtgatcggg
     2521 acacgcgacc ttgtacgcgc ggcccatgat ggtcacgtcg agggtcttcg gcttggcgct
     2581 catcgcgcgg ctattcgggg atctgcttca ggatgttttc caggcggacg cgcgcgccgt
     2641 cgatcttctc cgacagctgc ttcttgtccc cctgaagggc ggcgagcttc tggcgcaggt
     2701 cgcggttctc gtcgcgcagc ttctggcata gctgcgccgc ctgccggatc ttttgctcga
     2761 gcgtctcgag ttccgcgtcc atcggcacac tatagtttgg aaaatcctgt ggagtcaagg
     2821 tttaacgggt agttctcaat gtagattgtg atagcgctca cgcgccgagc gcagccccgg
     2881 ggtagaatcg gcgcgctcgc tcgaggtgtc ccggcgtcgt tctggcgccg gggtgaaacg
     2941 ggaaagaggt acggcgggaa accgccccaa gcctctgctg cccccgcaac ggtaagtgcg
     3001 cgagggcgta tcaatcgagc cgcggctgcg accgaacatc gccgcccgcg gcgccactgt
     3061 gagctgcgac tcacgggaag gcgatacgcc ccatcccacg agcccggaga ccggccccga
     3121 gcgatgagaa gaagcattcg ctcacgaacc gggcctgcgg ggacgctggc cgaaggggga
     3181 tccatgaaac gcaccatcgt cgcggcgtcg tgcctcgccg ccgcattcgc cgcttccacc
     3241 gttcacgccc aggaagacgc cgtcgtcgtg accgcgcagc gatttcccga gcggcgcctc
     3301 gacgcgccgg tcggcatgac ggtcatcacc gccgaggaaa tcgcgcgcga cacagcgcga
     3361 accgtgcccg aggtgctctc gcacctcggc gggctgcagg tgaggaacag ctcgggcacg
     3421 cccgatcagc agatcgatct gcgcggcttc ggcgtcaccg gcgaccagaa cacgctgatc
     3481 atgctcgacg gcgtgaggct gaacgaggcc gagctcagct cggccaagct ctcggcgatt
     3541 ccgctgcagt cgatcgagcg catcgagatc ctgcgcggct cgggcgccgt gctctacggc
     3601 gacaacgcgg tcggcgggac gatcaacatc gtcacgcgcg ggccgaagcc cgggcgcacc
     3661 ggcgacgtgc tggtcggggc cgggtcttac gggacctacg acgcgcgcgc gagcaccaac
     3721 gtcgcgaccg ggcgctcggg catcaccgcc tcggcggagc atctcgagag cgacaactac
     3781 cgcgcgaaca accgcctgcg ccaggacagc atgaccggcg actggcgcct cggcgacgag
     3841 cgcggcaacg tcggcttgaa gttcggcgcc gacacgcagc ggctgcagct gcccggcgcc
     3901 ttgtccgagg cgcagatcgc gaccgacccg cgcgccgcca gcacgccgaa caactggtcg
     3961 gcgcgcgaag gcgcgttcgc gatcctgtcg ctcgcccgtc cgctcggcaa cgcggacttc
     4021 ggggccgatc tcgcctaccg cgagaaggag tccacggcct tcttcgcggg cacgggcggc
     4081 tccccggcga cgtaccagga cacgcgcacg cgcaacctcg gcttctcgcc gcgcgtgcgc
     4141 gcgcccttcc ggctcggggg catcgagaac gtgctggtcg cgggcttcga ctggagcggc
     4201 tgggatttcg accggcgctt cgcgagctcg gtggacgccc tgggcgggcc cgacttcggc
     4261 acgggcggat cgcagcagag cgccgggacc tacgtccagt acagcggccg cctcgcgcag
     4321 ggcaccaagc tcaccgccgg ggcgcgcgag cagcgcgtcg tcgaccgcca ggtgccgctc
     4381 ggctttccgg gccccgagca gcgcgtcgtg cacacgatgg acgcgggcga ggtcgggctg
     4441 gagcaggcgc tggcggagcg cttcaccctg ttcggcaagt acggcaagag cttccgcatc
     4501 gcgaccgtcg acgagaacgg gttcacgctc accggcgagc cactgaagcc acagaccgcg
     4561 cgtagcaagg aggcgggcgt cgagtaccgt cgcagcgaca cgcgcttgcg cctgagcgcc
     4621 taccgcatcg atctcgacaa cgagatccac ttcaacccgc tcgccgcgcc gccgttcggc
     4681 gccaacgtca acctgccgcc cacgcggcgg cagggcgcgg agctgttcgc gagcgggcgg
     4741 ctcgtgccgg cgctcgaggt gtcgggcaac ttcatcgcgc agagcgcccg attcagaacg
     4801 ggagacttcg gcggcgcgga tctcgctggc aaccaggtcc cgctcgtgcc gcgccgtctc
     4861 gcgacgctgc gcgccgcgtg gcagcccgcg ccgggcacgg tggcgaccgt gtccacgcgc
     4921 tacgtcggcc gccagcgcta cgacaacgac caggcaaacc tcttccgcga gatgccgtcg
     4981 tactcggtga ccgatctcaa gattgcgcat cgccgcggac gcgccacctg ggggctcgcc
     5041 ttgaacaacg tcttcggcaa gcgctattac tcgtacgcca tcgtcgacag cccgaccgcg
     5101 ccgacgacgt tcaacgcgta ccccgaggcc ggccgcagcg tcatgggcac gctggagctc
     5161 gccttgtgag cgcatcgccg cgcctggtcg tcgccgcgcc gcacagcggc tcggggaaga
     5221 cgacgttcac gctcgggctg ctcgcggccc tggtgcgccg cgggctcgcg gtgcagccgt
     5281 tcaaggcggg gccggatttc atcgacccgt cgcaccacac ggcggtctgc ggccgggtct
     5341 cgcgcaacct cgatacgtgg atgtgcgacg acgcgacggt cgcggcgatc ttcgggcgcg
     5401 gtgcgcgcgg ggcggacgtc tcggtggtcg aaggcgtgat gggcctgtac gacggcttcg
     5461 ggcccgacga gcagcgcggc agcacggcgc acctggcgaa gctgctcgcg gcgccggtgg
     5521 tgctcgtcat cgacgcgcag cgcatggcga cgagcgcagc cgcggtggcg ctcggactgc
     5581 gcgagttcga ccgcggcgtc gcgatcgcgg gcgttgtctt caacaacgtc gggagcgaag
     5641 gccactacga atggctgcgc aaggcgctcg aggcgcgcac cgggctcgag agcttggggt
     5701 atctcctcca cgacccggcg ctgcggatcg acgagcggca tctcgggctc gtgcccgcgg
     5761 cggagcgcgc gctggcgaag gaggcgctcg cgcgctccgc cgaccggctc gagcgacacg
     5821 tggcgctcga tcgcatcctc gcggcggcgc gcgccccgcc ccggccgccc g
//
LOCUS       DATDMW010000254         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1074100, whole genome shotgun sequence.
ACCESSION   DATDMW010000254 DATDMW010000000
VERSION     DATDMW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074100"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..951
                     /locus_tag="VI319_11095"
     CDS             94..951
                     /locus_tag="VI319_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY6864433.1"
                     /translation="MRRRPELSSAARPLTHDVEAINERGEAVRQPIAGEHPLTLYIDK
                     REIVTLMTLGQAPEALAIGYLRNQRLARSLDEIAAVQVDWETDSVAITTRGGLGGLDD
                     KMSKRTVTTGCGQGTVFGDLMEEIDSVKLRDDVVLTDIGLHALLEAVRLRETVYKSAG
                     AVHGCALIENAPAADAKSVRLLYFVEDVGRHNAVDAIAGQMWLDDVDGSDKIFYTTGR
                     LTSEMVIKAAQMRIPFLVSRSGLTQMGHAIAERTGITMIGRAAGGKHYLLFTGRHRFR
                     RTTVPTLVA"
     gene            961..1545
                     /gene="mobA"
                     /locus_tag="VI319_11100"
     CDS             961..1545
                     /gene="mobA"
                     /locus_tag="VI319_11100"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEY6864434.1"
                     /translation="MAITGLILAGGRGSRMGGVDKGLQVLRGKPMIEWVLARFAPQVD
                     EVLINANQNVERYARLGPRVVPDDIGGYAGPLAGLHAGLKVAAHPLVATCPCDSPFLP
                     PDLVARLRAALEREAADLAVAKTGEQPHPVFSLVRRDALPGLARFLESGGRKIDAWYS
                     GLKTVEVRFDDEAEAFSNINTREELQAWDRLPPG"
     gene            1606..>2311
                     /locus_tag="VI319_11105"
     CDS             1606..>2311
                     /locus_tag="VI319_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEY6864435.1"
                     /translation="MATLKEVISCTSGYDPEALPVAKANEVIRGFVQPITDTETVPVR
                     EALGRVLAQEIVSPIDVPAHDNSAMDGYAVRNDDLGVHLPVTLTEVGVAYAGKAFAGE
                     VGRGECVRVMTGAVMPRNTDTVIVQEMVTAEGNRITIPPGQEPGQNRRLAGEDLKKGK
                     PALHAGRVLRPADLGLIASLGLGQATVRRRPRVAFFSTGDELVPVGKPLAPGQVYDSN
                     RYTIWGMLTRLGCEVID"
BASE COUNT          363 a          831 c          810 g          307 t
ORIGIN      
        1 tatctggcgc gcgcgcgccg accgctattc cgagatccgc accgccgaca ccgcgaaggc
       61 gcgcaagatc gagatgtcgt acataggcgg ctgatgcggc gccgtcccga gctcagcagc
      121 gccgcgcgcc cgctcaccca cgacgtcgag gcgatcaacg agcgcggcga ggcggtgcgc
      181 cagccgatcg ccggcgagca tcccctcacc ctgtacatcg acaagcgcga gatcgtcacg
      241 ctgatgacgc tcgggcaggc gcccgaggcg ctcgcgatcg gatacctgcg caaccagcgc
      301 ctcgcgcgct cgctcgacga gatcgccgcg gtgcaggtcg actgggagac cgattcggtc
      361 gcgatcacca cgcgcggggg cctgggcggc ctcgacgaca agatgagcaa gcgcaccgtc
      421 accacgggct gcggccaggg caccgtgttc ggcgacctca tggaggagat cgactcggtg
      481 aagctgcgcg acgacgtcgt gctcaccgat atcggcctgc acgcgctgct cgaggcggtg
      541 cggctgcgcg agaccgtata caagtccgcg ggcgccgtgc acggctgcgc cctgatcgag
      601 aacgctcccg cggccgatgc caagtcggtg cgcctgctgt acttcgtcga ggacgtcggc
      661 cgccacaacg ccgtcgacgc gatcgcgggc cagatgtggc tggacgacgt cgacggctcc
      721 gacaagatct tctataccac cgggcggctc acctcggaga tggtgatcaa ggcggcgcag
      781 atgcgcatcc cgttcctcgt gtcgcgctcg gggctcacgc agatgggcca cgccatcgcc
      841 gagcgcacgg gcatcaccat gatcggccgg gccgcgggcg gcaagcacta cctgctgttc
      901 acggggcggc accgcttccg caggaccacg gtgccgacgc tggtggcctg agcggccgcc
      961 atggcgatca cgggtctcat tctcgccggc ggacgcggca gccgcatggg cggcgtcgac
     1021 aagggcctgc aggtcctgcg cggcaagccg atgatcgaat gggtgctcgc gcgcttcgcg
     1081 ccgcaggtcg acgaagtgct gatcaacgcc aaccagaacg tcgagcgcta cgcgcggctc
     1141 ggccctcgcg tcgtgcccga cgacatcggc ggctacgccg gcccgctcgc gggcctgcac
     1201 gcgggactga aggtcgcggc gcacccgctc gtcgcgacct gtccctgcga ctcgccgttc
     1261 ctgccgcccg atctcgtcgc gcgcctgcgc gcggcgctcg agcgcgaggc agcggacctc
     1321 gccgtcgcga agacgggcga gcagccgcat cccgtgttct cgctggtgcg ccgcgacgcg
     1381 ctgccgggcc tcgcgcgctt cctcgagtcg ggcggccgca agatcgacgc ctggtactcg
     1441 ggcctgaaga ccgtcgaagt gcgcttcgac gacgaggccg aggccttctc caacatcaac
     1501 acgcgcgaag agctccaggc ctgggaccgg ctcccgcccg gttgacgcgg gcgggatgcg
     1561 ctatcgtatg accgtgtcaa cgccgggcga cgcggcgcaa cgcgaatggc caccctgaag
     1621 gaagtcatca gctgcaccag cggctacgat cccgaggccc tcccggtcgc gaaggcgaac
     1681 gaggtcatcc gcggattcgt gcagcccatc accgacacgg agaccgtgcc cgtgcgcgag
     1741 gccctgggcc gcgtgctggc ccaggagatc gtctcgccca tcgacgtgcc ggcgcacgac
     1801 aactcggcga tggacggcta cgcggtgcgc aacgacgacc tcggcgtgca cctgccggtg
     1861 acgctcaccg aggtcggcgt cgcctacgcc ggcaaggcct tcgcgggcga ggtcggccgc
     1921 ggcgaatgcg tgcgcgtgat gacgggcgcc gtgatgccgc ggaacacgga caccgtgatc
     1981 gtccaggaga tggtcaccgc ggaaggcaac cggatcacga tcccgcccgg acaggagccg
     2041 ggccagaatc gccggctcgc cggcgaggac ctgaagaagg gcaagcccgc gctgcacgcg
     2101 ggacgcgtgc tgcggcccgc cgacctcggg ctgatcgcat cgctcgggct cggccaggcg
     2161 accgtgcgcc ggcggccgcg cgtcgccttc ttctcgacgg gcgacgagct ggtgccggtc
     2221 ggcaagccgc tcgcgcccgg acaggtctac gactccaacc gctacacgat ctggggcatg
     2281 ctcacgcgcc tcggctgcga ggtgatcgac c
//
LOCUS       DATDMW010000255         2569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1199134, whole genome shotgun sequence.
ACCESSION   DATDMW010000255 DATDMW010000000
VERSION     DATDMW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199134"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VI319_11110"
     CDS             <1..576
                     /locus_tag="VI319_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY6864436.1"
                     /translation="GSCRTPMWQTARPETMDAIEDAAARLARAGASVRETALPREFEG
                     LCGERDVLGSVERSRAIAHEWRHHRDALSERMRRAVELGLATSDDDYRALLRLAHDCR
                     GKLAGLFADLDLLLVPCVPGEAPSGLESTGDPRFQRPWTALHAPAITLPTHEGPSGLP
                     VGIQLVGAMNADLRLLDAARWVWEQFGGESR"
     gene            573..1562
                     /locus_tag="VI319_11115"
     CDS             573..1562
                     /locus_tag="VI319_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864437.1"
                     /translation="MSRLLCAFVLAAAAAAASAPSTSSGQGYPSKPLRLVVPYAPGGV
                     VDFIGRTLAQRLSEHLGQSVIGDNRPGAGGIIGVEATAKSAPDGYTLVLMDPAIVINP
                     VLQDAVPYDVIRDLTAVSIVGSSPLVLVSNPALPVKNLTELIEYAKAHPGKLNFASAG
                     IGTTPHMAGELMKLRIGAQMTHVPYKGMGPAMADVVAGRVQFSFGSITSALPFLKDNR
                     IRGLATTGAKRSGALPDLPTMIEAGAGSGLSGFEVELWLGLFGPAGLPRDVLARLNAE
                     TKKAIEDPQARAAFAKVGVEPRGTSAGDAADFIRAENEKWGKVVRDAKLKGAG"
     gene            1562..2536
                     /locus_tag="VI319_11120"
     CDS             1562..2536
                     /locus_tag="VI319_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864438.1"
                     /translation="MAALPLSLACGPYDLLRPLVDGAARAPGIDFTVLTMASPERHGR
                     MLRHREFDVCELSLVGYLVARDQGAAFNAIPVFPHRRFRHGFVWKRAGCGVERPADLN
                     GRRVGLDTLQNSAGLWMRGILADHYGVDLKSIEWWCQEEEDVPFEPAPWMKVRRVAKG
                     GSIDQMLVDGELEAAIYPETLPSARAGSPKVGLLFPDPKAAEIEYYGKSGIFPIMHTV
                     VVRNEILEQHPWVAMSVVQAFERAKQLCYERMRDPRNLQLAWVREAMDEQQAVLGKDA
                     WPCGLVDSNRKALEAVVRYEHEQGMIGKRPPVEELFFPPSLQEIQHYL"
BASE COUNT          382 a          884 c          931 g          372 t
ORIGIN      
        1 ggcagttgcc gcacgccgat gtggcagacc gcgcgcccgg aaacgatgga cgcgatcgag
       61 gatgccgcgg cgcggctcgc gcgcgccgga gcgagcgtgc gcgagaccgc gctgccgcgg
      121 gaattcgagg gcctctgcgg cgagcgcgac gttctcggca gcgtcgagcg ctcgcgcgcg
      181 atcgcgcacg agtggcgcca tcaccgcgac gccttgagcg agcgcatgcg gcgcgcggtc
      241 gagctcggcc tcgccacgtc cgacgacgac taccgggcgc tcctgcgcct cgcgcacgac
      301 tgccgcggga agctcgccgg gctgttcgcc gatctcgacc tgctgctcgt cccctgcgtt
      361 ccgggcgagg caccctcggg cctggagtcg acgggcgatc cgcggttcca gcggccgtgg
      421 accgcgctgc acgcgcccgc gatcacgctg ccgacgcacg aaggcccgag cggattgccg
      481 gtcgggatcc agctggtcgg ggcgatgaac gccgatctgc ggctgctcga cgcggcgcgc
      541 tgggtgtggg aacaattcgg aggagaatct cgatgagcag gcttctttgc gcgttcgttc
      601 tcgcggccgc ggcggcggcc gcatccgccc cttcgacaag ctcgggacag gggtatccgt
      661 cgaagccgct gcgcctggtc gtgccctacg cgcccggcgg cgtggtcgac ttcatcggcc
      721 gcacgctcgc ccagcgcctg tccgagcacc tcggccagag cgtgatcggc gacaaccgtc
      781 ccggcgcggg cggcatcatc ggcgtcgagg cgacggcgaa gtcggcgccc gacggctaca
      841 cgctcgtcct catggacccg gcgatcgtga tcaacccggt gctgcaggac gcggtgccct
      901 acgacgtgat ccgcgacctc accgcggtct cgatcgtggg ctcgtcgccg ctcgtgctcg
      961 tgagcaatcc cgcgctgccc gtgaagaacc tgaccgagct gatcgaatac gcgaaagcgc
     1021 acccgggcaa gctcaacttc gcctccgccg ggatcggcac gacgccgcac atggccgggg
     1081 agctgatgaa gctgcggatc ggggcgcaga tgacgcacgt gccgtacaag ggcatggggc
     1141 cggcgatggc ggacgtggtc gcgggccgcg tgcaattcag cttcggcagc atcacgtcgg
     1201 cgctgccgtt cctcaaggac aaccgtatcc gcggcctggc gacgacgggc gcgaagcgct
     1261 cgggcgccct gcccgacctg ccgacgatga tcgaggccgg cgccgggtcg ggactgagcg
     1321 gcttcgaggt cgagctgtgg ctcggcctgt tcgggcccgc gggcctgccg cgcgacgtgc
     1381 tcgcgcgcct gaacgccgag acgaagaagg cgatcgagga tccgcaggcc cgcgccgcct
     1441 tcgccaaggt cggcgtcgag ccgcgcggaa ccagcgcggg ggacgcggcc gacttcatcc
     1501 gcgccgagaa cgagaaatgg ggcaaggtcg tgcgcgacgc gaagctgaag ggagcgggct
     1561 gatggccgct ctgccgctgt cgctcgcctg cggtccgtac gacctgctgc gcccgctggt
     1621 cgacggcgcg gcgcgtgcac cgggcatcga cttcaccgtg ctcaccatgg cgtcgcccga
     1681 gcgccacggg cggatgctgc gccaccgcga gttcgacgtg tgcgagctgt cgctggtcgg
     1741 ctatctcgtc gcgcgcgacc agggcgcggc gttcaacgcg atcccggtgt tcccgcaccg
     1801 gcgcttccgc cacggcttcg tctggaagcg cgcgggctgc ggcgtcgagc ggcccgccga
     1861 cctcaacggg cggcgcgtcg gcctcgacac gctgcagaac tccgcgggcc tgtggatgcg
     1921 cggcatcctc gccgaccact acggcgtcga cctgaaatcg atcgagtggt ggtgccagga
     1981 agaggaggac gttcccttcg agcccgcgcc gtggatgaag gtgcgccgcg tcgcgaaggg
     2041 cggcagcatc gaccagatgc tcgtcgacgg cgagctcgag gcggcgatct atcccgagac
     2101 gctgccctcg gcgcgcgccg ggtcgccgaa ggtgggcctg ctctttcccg acccgaaggc
     2161 ggccgagatc gagtactacg ggaagagcgg catcttcccg atcatgcaca cggtcgtggt
     2221 gaggaacgag atcctcgagc agcatccctg ggtcgcgatg agcgtggtgc aggcgttcga
     2281 gcgcgcgaag cagctgtgct acgagcgcat gcgcgatccg cgcaacctgc agctcgcgtg
     2341 ggtgcgcgag gcgatggacg agcagcaggc cgtcctcggc aaggacgcgt ggccctgcgg
     2401 cctcgtcgat tccaaccgca aggcgctcga ggcggtcgtg cgctacgagc acgagcaggg
     2461 aatgatcggg aagcgcccac cggtcgaaga gctcttcttc ccgccgagcc tccaggagat
     2521 ccagcactat ctttaattcg gggccagggt cgtaattatt ttcacccta
//
LOCUS       DATDMW010000256         4947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1946557, whole genome shotgun sequence.
ACCESSION   DATDMW010000256 DATDMW010000000
VERSION     DATDMW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946557"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VI319_11125"
     CDS             complement(<1..533)
                     /locus_tag="VI319_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864439.1"
                     /translation="MNTSLPTEKKILVVEDEAGIRNNILLMLKIERFTAVGAENGRVG
                     LELARRHQPDLILCDVNMPEMDGFEVLETLRAEPGFADTPFVFLTALDDRASLRRGMN
                     LGADDYLTKPFTRDELMAAVNARLRKHETSTQALAARLLTKAASLKEKFRASTVGEDG
                     WGPLLDVDPAETTGKIA"
     gene            complement(530..2287)
                     /locus_tag="VI319_11130"
     CDS             complement(530..2287)
                     /locus_tag="VI319_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6864440.1"
                     /translation="MSDAPASALLGAFSSSDFLPQRVSLLYRLSGRDIPAILPASLVV
                     AFGLWGNVADAMLAAWIVWLLVASLARLGLGRAYRIRRPGPKAAARWESYFCLASAAV
                     GMAWGITVLFLYPERAQLHEILVPFLIGSVAMGLPATLAPSPKAFACLIAPILAPMIG
                     VLYSQGGAFNTSAAILILVFSAVLLALYLSSHHALLETLRFGRENELLLEKLTRAKDR
                     LDLALRASSVLIWEWDGRRGDMILEGRWAEVLGYHESTVRRLEDVMRLFHPEDLPTLR
                     RALVECMRGATPEYAAEHRVRTPSGDWRWSLSSGRVLERDAEGRAVRMIGTNVDIERQ
                     KRAEAELLTAVQREKELSELKSKFLSMASHEFRTPLATILSSSELLERYAERIDPQEK
                     ASLLQSIESGAKRMNELINDVLTIGRAESGVLRLNPARLDLRDLCARVIREFRIAQGR
                     SHVVRFNDRFDLPAVEMDERLLRHILENLLSNAAKYSPEGSEILVTLARRAEDVFIDV
                     QDHGIGIPEGDQARLFESFHRASNVENRPGTGLGLAIVRKAVDLHGGSISFRSAPGKG
                     THFTVCLPVYSRAAALTAS"
     gene            2530..3681
                     /gene="carA"
                     /locus_tag="VI319_11135"
     CDS             2530..3681
                     /gene="carA"
                     /locus_tag="VI319_11135"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY6864441.1"
                     /translation="MSAAQTPAVLALADGTVFRGRSIGADGLSAGEVVFNTAITGYQE
                     ILTDPSYCRQIVTLTYPHIGNTGVNGEDNEAAQVVAAGLVIRDLPLLASSWRAEQPLP
                     DFLRERNVVAIADIDTRRLTRILREKGAQNGAVMAGAIDERRALEAARGFPGLAGMDL
                     AKVVSTREPYEWVEGGWALGRGYAGPGRERFHIAAYDYGIKRNILRMLADRGCRITVL
                     PAQAPARDALALKPDGVFLSNGPGDPEPCDYAIRAIREIIDRGIPTFGICLGHQLMGL
                     ASGAKTIKMKFGHHGANHPVKDLDTGAVAITSQNHGFAVDPATLPANLRATHVSLFDG
                     SLQGLARTDRPAFCFQGHPEASPGPHDIGYLFDRFADLMQKHRERRDAA"
     gene            3683..>4947
                     /gene="carB"
                     /locus_tag="VI319_11140"
     CDS             3683..>4947
                     /gene="carB"
                     /locus_tag="VI319_11140"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY6864442.1"
                     /translation="MPKRTDIRSILIIGAGPIVIGQACEFDYSGAQACKTLRAEGYKV
                     ILVNSNPATIMTDPEMADVTYVEPINPQMLEAIIERERPDALLPTMGGQTALNCALDL
                     ARAGVLEKHGVEMIGASPQAIDTAEDRDKFKQAMARIGLECPHSQLAHSMEEALQVQA
                     GIGFPVVIRPSFTLGGSGGGIAYNREEFVAICERGLDASPAHQLLIEESVIGWKEFEM
                     EVVRDRKDNCIIICSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRDASIAVLREIG
                     VDTGGSNVQFAINPVDGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGDTLDE
                     LKNDITGGATPASFEPSIDYVVTKVPRFAFEKFPLTDSTLTTQMKSVGEAMAIGRTFK
                     EAFLKGVRSLELGKEGMLLTQAGDDEDDTS"
BASE COUNT          827 a         1696 c         1654 g          770 t
ORIGIN      
        1 tcggcgatct tgccggtcgt ctcggcgggg tcgacgtcga gcagcggccc ccagccgtcc
       61 tcgcccacgg tgctcgcgcg gaacttctcc ttgaggctcg ccgccttcgt aagcagccgc
      121 gcggccaacg cctgcgtcga cgtctcgtgc ttgcgcagcc gcgcgttcac cgccgccatc
      181 agctcgtcgc gcgtaaacgg cttcgtgagg taatcgtcgg cgcccagatt catgcctcgg
      241 cgcaagctgg cgcggtcatc gagcgcggtg agaaaaacga acggggtgtc ggcgaagccc
      301 ggctcggcgc gcagcgtctc caggacctcg aagccgtcca tctccggcat gttgacgtcg
      361 cagaggatga ggtcgggctg gtgccgccgc gcaagctcga gcccgacgcg cccgttctcc
      421 gcgccgacgg cggtgaagcg ctcgatcttg agcatgagca ggatgttgtt ccggatcccg
      481 gcctcgtcct cgacgaccag gatcttcttc tcggtgggca gggaggtgtt catgatgccg
      541 tcagtgctgc agcccgcgaa tacaccggca gacagacggt gaagtgcgtg cccttgcccg
      601 gcgcgctcct gaaagagatg cttcccccgt gcaggtccac cgccttccgg acgatcgcga
      661 ggccgagccc cgttccggga cggttctcga cgttggacgc gcggtgaaag ctctcgaaca
      721 gtcgcgcctg gtcgccctcg gggatgccga tgccgtggtc ctggacgtcg atgaacacgt
      781 cttcggcgcg gcgcgcgagc gtcaccagga tctcgctgcc ttcgggcgag tacttcgccg
      841 cgttcgagag caggttctcc aggatgtggc gcagcagccg ctcgtccatc tccaccgccg
      901 gcaggtcgaa ccggtcgttg aagcggacga cgtgcgaccg gccttgcgca atccggaatt
      961 cgcgtatgac gcgcgcgcac aggtcgcgca gatccagccg cgcggggttc aggcgcaaca
     1021 cgcccgactc cgcgcgcccg atcgtgagca cgtcgttgat cagctcgttc atgcgcttcg
     1081 cgcccgactc gatgctctgc agcaggctcg ccttttcctg cggatcgatg cgctcggcgt
     1141 agcgctcgag cagctcggag gacgacagga tggtcgcgag cggcgtgcgg aactcgtgcg
     1201 acgccatcga caggaacttc gacttcagct cggagagctc cttctcgcgc tgcacggcgg
     1261 tcagcagctc ggcctcggcg cgcttctgcc gctcgatgtc gacattggtt ccgatcatgc
     1321 gcaccgcgcg tccctcggcg tcccgctcga ggacccggcc gctgctcagg ctccagcgcc
     1381 agtcccccga cggcgttcgg acgcggtgct ccgcggcgta ctcgggcgtc gcgcccctca
     1441 tgcactccac cagggctctg cgcagcgtcg gcaggtcctc gggatggaag agccgcatga
     1501 cgtcctccag ccgccgcacc gtggactcgt ggtatccgag cacctcggcc cagcgtcctt
     1561 caaggatcat gtcgccgcgg cggccgtccc attcccagat cagcacgctc gaggcacgca
     1621 gcgcgagatc gaggcggtcc ttggcgcgcg tcaatttctc gagcagcagc tcgttctcgc
     1681 gcccgaagcg cagcgtctcg agcagcgcat ggtgcgacga gaggtacagc gcgagcagca
     1741 cggcggagaa caccaggatg aggatcgccg ccgaggtgtt gaacgcgccg ccttgcgagt
     1801 acagcacgcc gatcatcggc gcgaggatgg gtgcgatgag gcaggcgaag gccttggggc
     1861 tgggggcgag cgtcgcgggc agccccatcg cgacgctgcc aatcaggaac gggacgagga
     1921 tctcgtgcag ctgggcccgt tccgggtaca gaaacaatac cgtgatcccc cacgccatgc
     1981 cgacggcggc gctcgccagg cagaagtagc tctcccagcg cgcggccgcc ttcgggcctg
     2041 ggcggcggat ccggtacgcg cggccaagac cgaggcgcgc gaggctggcg acgagcagcc
     2101 agacgatcca tgccgcgagc atggcatccg cgacgttgcc ccaaaggccg aaggcgacca
     2161 cgagggacgc gggcaggatc gcgggtatgt cgcggccgga aagccgataa agaagcgaca
     2221 cccgctgcgg caggaaatcc gagctggaaa acgcgcccag cagcgcgctc gcgggtgcgt
     2281 ccgacattct tcttttgcgg gaccccgtcg ggtccctccc tgttctggtt cggcgtccgg
     2341 aagcccggat gccgtctcat tgtgacaccg ttgcgactat aagcaacgtg ctaagcaaca
     2401 tctgcgccaa gaaccgccga agcgattgat gcgacagcgt cttccgggta taatttcgtg
     2461 gcttaaccga gagcgggatc cgcgcaagcc gatcccgctt tgtacatcta caccccgagg
     2521 acgcctgcct tgtccgccgc ccagacgccc gccgtgctcg cgctcgccga cgggaccgta
     2581 ttccgcggcc ggtcgattgg cgccgacggg ctctcggcgg gcgaagtcgt cttcaacacc
     2641 gcgatcaccg ggtaccagga aatcctgacc gacccgtctt actgtcggca gatcgtcacg
     2701 ctgacgtacc cgcacatcgg caacaccggc gtcaacggcg aggacaacga ggcggcgcag
     2761 gtcgtcgccg caggcttggt gatccgtgat ctgccgctgc tcgcctcgag ctggcgcgcc
     2821 gagcagccgc ttccggattt cctgcgcgag cgcaacgtcg ttgcgatcgc cgatatcgat
     2881 acgcgccggc tcacgcgaat cctgcgcgag aagggtgcgc agaacggcgc cgtcatggca
     2941 ggcgcgatcg acgagcggcg cgcgctcgaa gcggcgcgcg gcttccccgg cctcgccggg
     3001 atggatctcg cgaaggtcgt gagcacgcgc gagccgtacg agtgggtcga gggcggctgg
     3061 gcgctcggcc gcggttacgc cgggcccggg cgcgagcgct tccatatcgc cgcctacgac
     3121 tacgggatca agcgcaacat cctgcgcatg ctcgccgacc gcggctgccg catcacggtt
     3181 ctgcccgcgc aggcgccggc gcgcgacgcg ctggcgctga agcccgatgg cgtgttcctg
     3241 tcgaacggcc cgggcgatcc cgagccttgc gattacgcga tccgcgcgat ccgcgagatc
     3301 atcgaccgcg gcatcccgac cttcggcatc tgtctcgggc atcagctcat ggggctcgcc
     3361 tcgggtgcga agacaatcaa gatgaagttc ggccatcacg gtgcgaacca ccccgtgaag
     3421 gacctcgaca cgggcgcggt ggcgatcacc agccagaacc acggcttcgc cgtcgatccc
     3481 gcgactctgc cggcgaacct gcgagcgacg cacgtgtcgc ttttcgacgg cagcctgcag
     3541 gggctcgcgc gcaccgaccg tccggcgttc tgcttccagg gccacccgga ggcgagcccg
     3601 gggccgcacg acatcggtta ccttttcgac cgcttcgccg acctgatgca gaagcaccgc
     3661 gagcggcgcg acgccgcctg agatgccgaa gcgcaccgac atccgcagca tcctgatcat
     3721 cggcgccggt ccgatcgtca tcggacaggc gtgcgagttc gactattccg gcgcgcaggc
     3781 ttgcaagacg ctgcgcgccg agggctacaa ggtgatcctg gtgaactcca accccgccac
     3841 gatcatgacg gaccccgaga tggccgacgt cacgtacgtc gagccgatca atccgcagat
     3901 gctcgaggcg atcatcgagc gcgagcgccc ggacgcgctg ctgccgacga tgggcgggca
     3961 gaccgcgctc aactgcgcgc tcgacctcgc gcgcgccggc gtgctcgaga agcacggcgt
     4021 cgagatgatc ggcgcaagcc cccaggcgat cgacaccgcc gaggaccgcg acaagttcaa
     4081 gcaggcgatg gccaggatcg gcctcgaatg cccgcactcg cagcttgccc actcgatgga
     4141 ggaggcgctg caggtgcagg ccgggatcgg cttcccggtc gtcatccggc cgtcgttcac
     4201 gctgggcgga tccggcggcg gcatcgcgta caaccgcgag gagttcgtcg cgatctgcga
     4261 gcgcgggctc gacgcttcgc ccgcgcacca gctgctcatc gaggagtcgg tgatcggctg
     4321 gaaagagttc gagatggagg tggtgcgcga ccgcaaggac aactgcatca tcatctgctc
     4381 gatcgagaac ctcgacccga tgggcgtcca caccggcgac tcgatcaccg tcgcgccggc
     4441 ccagacgctg accgacaagg aataccagat catgcgcgac gcctcgatcg cggtgctgcg
     4501 cgagatcggc gtcgataccg gcggctcgaa cgtgcagttc gcgatcaatc cggtcgacgg
     4561 ccgcatgatc gtgatcgaga tgaacccgcg cgtgtcgcgc tcgtcggcgc tggcgtcgaa
     4621 ggccaccggc ttcccgatcg ccaaggtggc ggccaagctg gccgtcggcg acacgctgga
     4681 cgaactgaag aacgacatca ccggcggcgc cacgccggcg tccttcgagc cctcgatcga
     4741 ctacgtggtc accaaggtgc cgcgttttgc cttcgagaaa ttcccgctga ccgactccac
     4801 cctgaccacc cagatgaagt cggtgggcga ggcgatggcg atcgggcgga cgttcaagga
     4861 agcgtttttg aaaggcgtcc gttcgctcga gctcggcaaa gagggcatgc tgctgacgca
     4921 ggccggcgac gacgaagacg acacgtc
//
LOCUS       DATDMW010000257         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2196979, whole genome shotgun sequence.
ACCESSION   DATDMW010000257 DATDMW010000000
VERSION     DATDMW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196979"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2032
                     /locus_tag="VI319_11145"
     CDS             <1..>2032
                     /locus_tag="VI319_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HEY6864443.1"
                     /translation="DLGRLRAHLPVPETLRSGVGSVRVWTQFTRDGVDELVADLAMRD
                     ARAQLSADALPLELASISGRARYRAQPTGFTFTTEGLRFRLPSGLEAQPGNFALARKA
                     EPGRDPQSEVRADGIDLKIAATLLDYFPVPPDVKGRALRFAPRGQLTDAVVTWSGDEA
                     RPARSYALHGRFRDLAVNAVDSIPGVSGLSGEIEGSEAGGTLRVTGTNARFEQLHVFR
                     APLAIDRLEARAHWKHEGGATRVTIEQASFANEDAEGSVTGSWRSLPESKTHSPGYID
                     LKGHLTRAKATSVARYMPNTTAPVRDWLERAIRSGDSSRATFELKGDLYEFPFGRGSN
                     GHFLVEGDIHDGSLRYLPDWPGVEAVEGTLRFENRRMEIRAKSARIFSSHATAVAAVI
                     EDLGAKPPLLTIDGDVDTTGADGLRFLRESPLVNGPGSFTKAVNVEGPGRLKLHLDIP
                     LGGADPVRVAGDYQFNGDVATTAGTLALRDLRGRLSFTERGVRAPDLAGTLFGQPAQI
                     AISSQPDGQVATTVQGRIDAANLAAYVPEAIAARATGATEWKARLLSSRAASELTLSS
                     DLKGLAVALPAPLAKSADESRPLAINIARLGSGNEAVTTVLDGSVHGRFNRIAPERWQ
                     AALRFGAPLSSEPLREGMWLYGELPVLDVDAWQAVFKAADPAAPAAPVELRGL"
BASE COUNT          281 a          738 c          750 g          263 t
ORIGIN      
        1 gacctgggcc gcctgcgcgc gcacctgccc gtgcccgaga cgctgcgcag cggcgtgggc
       61 agcgtgcgcg tgtggaccca attcacccgc gacggcgtgg acgagctggt ggcggacctc
      121 gcgatgcgcg atgcgcgcgc gcagctctcg gccgacgcgc tgccgctgga gctcgccagc
      181 atctcgggac gcgcgcgcta tcgcgcgcag cctacgggct tcaccttcac caccgagggc
      241 ctgcgcttcc ggctgccttc gggcctcgag gcgcagccgg gcaacttcgc gctggcgcgc
      301 aaggcggagc cggggcgcga cccgcagtcg gaggtgcgcg ccgacggcat cgacctgaag
      361 atcgcggcga cgctgctgga ctacttcccg gtgccccccg acgtgaaggg ccgggcgttg
      421 cgcttcgcgc cgcgcggcca gcttaccgac gcggtcgtca cgtggtcggg cgacgaggcg
      481 cggccggcca ggtcgtacgc gcttcatggg cgcttccgcg acctcgcggt gaacgcggtg
      541 gactcgattc ccggcgtgag cggcctgagc ggcgagatcg agggctccga ggcgggaggc
      601 acgctgcgcg tcacgggcac gaacgcgaga ttcgagcaat tgcacgtctt ccgcgcgccg
      661 ctcgcgatcg accgcctgga ggcgcgcgcc cactggaagc acgagggcgg cgcgacgcgc
      721 gtgacgatcg agcaggcgag cttcgccaac gaggacgcgg agggcagcgt cacgggctcg
      781 tggcgctcgc tgccggaatc gaagacgcat tccccgggct acatcgacct caagggccac
      841 ctcacgcgcg cgaaggccac cagcgtcgcg cgatacatgc ccaacaccac ggcgccggtt
      901 cgcgactggc tggagcgcgc gatccgctcc ggcgactcct cgcgcgccac attcgagctc
      961 aagggcgacc tctacgagtt cccgttcggc cgcggatcga acggccactt cctcgtcgag
     1021 ggcgacatcc acgacgggtc gctgcgctac ctgccggact ggccgggcgt cgaggcggtc
     1081 gagggcacgc tgcgcttcga gaaccggcgc atggagatcc gcgcgaagag cgcgcgcatc
     1141 ttctccagcc atgcgaccgc cgtggcggcg gtcatcgagg acctcggcgc gaagccgccg
     1201 ctgctcacga tcgacggcga cgtggatacc accggcgcgg acggcctgcg cttcctgcgc
     1261 gagtcgccgc tcgtgaacgg gccgggctcc ttcacgaagg cggtgaacgt cgaggggccg
     1321 gggcggctca agctgcacct ggacatcccg ttgggcggcg ccgatcccgt gcgcgtcgcc
     1381 ggcgattacc agttcaacgg cgacgtcgcc acgacggcgg gcacgctcgc gctgcgcgac
     1441 ctgcgcgggc ggctgtcctt caccgagcgc ggcgtgcgcg cgccggacct cgcgggcacg
     1501 ctcttcgggc agccggcgca gatcgccatt tcctcgcaac ccgacgggca ggtggccacg
     1561 acggtgcagg gacgcatcga cgccgcgaac ctcgccgcgt acgttcccga agccatcgcc
     1621 gcgcgcgcca cgggcgcgac ggaatggaag gcacggctgc tgtcgagccg cgcggcgagc
     1681 gagctcacgt tgagctcgga cctcaagggc ctcgccgtgg cgctgcccgc accgctcgcc
     1741 aagagcgccg acgaaagccg gccgctcgcg atcaacatcg cgcggctggg ctcgggcaac
     1801 gaggccgtga cgaccgtgct cgacggcagc gtgcacggcc ggttcaaccg catcgcgccc
     1861 gagcgctggc aggcggcgct gcgcttcggg gcgccgctgt cgtccgagcc gctgcgcgag
     1921 ggcatgtggc tctatggcga gctgcccgtg ctcgacgtcg atgcgtggca ggccgtcttc
     1981 aaggcggcgg accctgccgc gcccgcggcg ccggtggagc tgcgcggcct gg
//
LOCUS       DATDMW010000258         3840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_576774, whole genome shotgun sequence.
ACCESSION   DATDMW010000258 DATDMW010000000
VERSION     DATDMW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576774"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VI319_11150"
     CDS             <1..858
                     /locus_tag="VI319_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HEY6864444.1"
                     /translation="QLFEEMVYVLSGRGSTQIWNDAGQRSSFEWKAGALFAIPLNTWH
                     QHFNGSGQEPARYVAVTNAPVVINLYEDTDFVFNTKHDFKGRFSGEPDYFANKGEQKG
                     FLLATNFVADAVNLPLIAAGERGAGGGHIRFNLARGSLPAHISQFPVGTYKKAHAHGP
                     GAHVILLSGEGYSLMWPEGDEPRRYEWEVGTMIVPPNMWMHQHFNAGSTPARYLAFKY
                     EGVALRNAQGVPKAWISRRIGGDQIDYADEKPAVRRMFADALAKRGLQPRMDKAYEAE
                     LEDLPPKAA"
     gene            1051..2253
                     /locus_tag="VI319_11155"
     CDS             1051..2253
                     /locus_tag="VI319_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY6864445.1"
                     /translation="MLRKKAVALAVAGALGTPVAALAQNVQVYGKLYPQLGMFKTSGA
                     SSAADLNPALTTGNGKDFKSRINLDASNTRIGFRGSEDLGGGLKAIFQIESKVSIDTG
                     GTLWASRDSFVGLTGGFGTVKLGNMDTVYKSLGDPIGILGISSGNFVSDSAVLSQGPL
                     DGIDFHRREKNSFVYESPSFGGVTLLAGYSIPKEEEAKAAGDNRHPKVESFGVKYEVG
                     PLYIAAAHEIRKDQFAFSSSPQVAGGPFTNPTSSAGGVHSNDTATRAAVMYEFPTKTK
                     VSLDVAQIKLEETGGAVGKFNSAKNNRWAVTGEQGLGNIKLAASYAKSDKGSCELNGG
                     GACTTDGFDGSQLNVGAMLSLSKRTGLFAIYSRLTTGTSASFNNTPVDISPGSDSTQA
                     AIGVTHSF"
     gene            2381..3427
                     /locus_tag="VI319_11160"
     CDS             2381..3427
                     /locus_tag="VI319_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6864446.1"
                     /translation="MRLLARVGAAAIAACSFSAAAQPQAKDASGIAQYQGADRAQRLL
                     QGAKKEGELSVYTSAPVDDYAVFSAAFEKKYGVKVRVWRASSEKVQQRGVTEARAGRF
                     DVDIFDTNGPEMESLHREKILQEVKSPYLADLIPQAIMPHREWVGTRLNIFALAYNTR
                     LVKKDELPKNWEDLLDPRWKGRLGIEAADEDWLAGVVTQLGEQKGLQLFRDIATKNGF
                     SVRKGHTLLTNLVAAGEVPLALTVYNYKAEQLKQRGAPIDWFVIPPAVARPNGIGMAR
                     RARHPSAAVLFYDFMISDAQFLLLERDFVPTSRKIKTKLNQFPLKFVDSSDMLDHYAK
                     WTRLYEEIITRQGR"
     gene            3445..>3840
                     /locus_tag="VI319_11165"
     CDS             3445..>3840
                     /locus_tag="VI319_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6864447.1"
                     /translation="MGKESHYSSKKDRVFVRAIAGQYNLKDELKRLRSLPRVIKGREL
                     KFQDGPQSFSKHFVEPVDGMTQTLHIHLEEYAPGGRTQKHGHVNEAAFYILDGRGYEI
                     HDGVRYDWQAGDVAIVHNNCVHQHHNADPE"
BASE COUNT          740 a         1226 c         1287 g          587 t
ORIGIN      
        1 cagctgttcg aggagatggt ctacgtgctc tcggggcgcg gctcgacgca gatctggaac
       61 gatgcgggcc agcgctcgag cttcgaatgg aaggcgggcg cgctgtttgc gatcccgctc
      121 aacacctggc accagcattt caacggctcg gggcaggagc ccgcgcgcta cgtcgccgtg
      181 accaacgcgc cggtcgtgat caacctgtac gaggacacgg acttcgtgtt caacacgaag
      241 cacgacttca agggccgctt ctccggcgag cccgactatt tcgcgaacaa gggcgagcag
      301 aagggcttcc tgctcgcgac caacttcgtc gccgacgccg tgaacctgcc gctgatcgcg
      361 gcgggcgagc gcggcgcggg cggcggccac atccgcttca acctcgcgcg cggctcgctg
      421 ccggcgcaca tctctcagtt cccggtcggc acgtacaaga aggcgcacgc gcacggcccc
      481 ggcgcgcacg tcatcctgct ctcgggcgag ggctacagcc tgatgtggcc cgagggcgac
      541 gagccgcggc gctacgagtg ggaggtcggg acgatgatcg tgccgcccaa catgtggatg
      601 caccagcatt tcaacgccgg atcgacgccg gcgcgctatc tcgcgttcaa gtacgagggc
      661 gtggcgttgc gcaacgcaca aggcgtcccg aaagcgtgga tcagccgtcg catcggcggc
      721 gaccagatcg actatgcgga tgaaaaaccg gcggttaggc gaatgttcgc ggacgcgctc
      781 gcgaagcgcg ggctgcagcc gcgcatggac aaggcgtacg aagccgagct cgaggatctc
      841 ccgcccaagg cggcttgatg tcgatcacgt tgcgtaaacg caacagagcg ggcgtcagcc
      901 cattgacagg gacatagtcc actcgtacgc tgcctctaac gtcctgattt tgtagggcgg
      961 agttccgcat agaggaacgg aacttgaggc gggcggcaac atggccccgt ccgaagaaat
     1021 ctgacagcac ctagaactat aggaggggaa atgctcagga aaaaagcggt cgccctggca
     1081 gttgccggcg cgctgggaac acccgtcgcc gcgctcgcgc agaacgtgca ggtctacgga
     1141 aagctgtatc cgcagttggg gatgttcaag acctcgggcg catcgagcgc cgccgatctg
     1201 aatcccgccc ttacgacggg taacgggaag gacttcaaga gccggatcaa cctcgacgcc
     1261 agcaacacca gaatcggctt tcgtggctcg gaggatctcg gcggcggtct gaaggcgatc
     1321 ttccagatcg agagcaaggt gtcgatcgat accggcggca cgctctgggc cagccgcgac
     1381 tcgttcgtcg gcctcaccgg cgggttcggc acggtgaagc tcggcaacat ggatactgtg
     1441 tacaagtcgc tgggcgaccc gatcgggatt ctcgggatca gcagcggcaa cttcgtctcc
     1501 gacagcgcgg tgctgtcgca ggggccgctc gacggcatcg acttccacag gcgcgagaag
     1561 aactcgttcg tctacgagtc accctcgttc ggcggcgtca cgctgctggc cggctattcg
     1621 atcccgaagg aggaagaagc gaaggccgct ggcgacaacc gtcatcccaa ggtggagtcg
     1681 ttcggcgtga agtacgaggt cgggccgctc tatatcgccg ctgcgcacga gatccgcaag
     1741 gaccagttcg cgttctcgag ttccccgcag gtcgcgggcg ggccgttcac gaacccgaca
     1801 tcgagcgccg ggggggtgca ctcgaacgac actgcgaccc gcgccgcggt catgtacgag
     1861 ttcccgacca agaccaaagt gtcgctggat gtcgcgcaga tcaagctgga ggagaccggc
     1921 ggtgcggtcg gcaagttcaa cagcgcgaag aacaatcgct gggccgtcac gggcgagcag
     1981 ggtctgggca acatcaagct cgcggccagc tacgcgaagt cggacaaggg ctcgtgcgag
     2041 ctgaacggcg gcggcgcgtg caccaccgac gggttcgacg gaagccagct gaacgtcggc
     2101 gcgatgttga gcctgtccaa gcgcacgggc ttgttcgcga tctactcgcg gctcacgacc
     2161 ggcacgtcgg cgtcgttcaa caatacgccg gtcgatatca gccccggttc ggacagcacg
     2221 caggcggcga tcggcgtcac gcactcgttc taaagtaagc ggttcgagcg gcccggcggc
     2281 gcgttcgcgc tccgggccgt tttcatttgc tgcggtcccc ggggcctgac tcttcgccgg
     2341 ctggtcgggt cgaacttccg aggagagact tgtgggctcc atgaggcttt tggcgcgtgt
     2401 gggcgccgcc gcgatcgccg cgtgctcctt ttcggcggca gcacaaccgc aggcgaagga
     2461 cgcctccggg atcgcccaat accagggcgc cgaccgcgcc cagcgcctgc tgcagggcgc
     2521 gaagaaggag ggcgagctca gcgtctacac ctccgcgccc gtggacgatt acgccgtgtt
     2581 ctctgccgcg ttcgagaaga agtacggcgt caaggttcgc gtctggcgcg cgtcctccga
     2641 gaaggtgcag cagcgcggcg tgaccgaggc gcgcgcgggg cgcttcgacg tcgacatctt
     2701 cgacaccaac ggccccgaga tggagtcgct gcaccgcgag aagatcctgc aggaggtgaa
     2761 gtcgccctat ctcgcggacc tgatcccgca ggcgatcatg ccgcaccgcg agtgggtcgg
     2821 gacacggctc aacatcttcg cgctcgccta caacacgcgg ctggtgaaga aggacgagct
     2881 gccgaagaat tgggaggatc tgctcgatcc gcgctggaag ggacgcctcg ggatcgaggc
     2941 cgcggacgag gactggctcg cgggcgtcgt cacgcagctc ggcgagcaga agggcctgca
     3001 gctgttccgc gacatcgcca cgaagaacgg cttctcggtg cgcaagggtc acacgctgct
     3061 cacgaacctg gtcgccgcgg gcgaggtgcc gctcgcgctc acggtctaca actacaaagc
     3121 cgagcagctg aagcagaggg gcgcgccgat cgactggttc gtgatcccgc ccgcggtcgc
     3181 gcgtcccaac gggatcggca tggcgcgccg cgcgcggcat ccgagcgccg cggtgctctt
     3241 ctacgacttc atgatctcgg acgcgcagtt cctcctgctg gaacgcgact tcgtcccgac
     3301 gagccggaag atcaagacca agctcaacca attcccgcta aaattcgtcg actcgagcga
     3361 catgctcgac cactacgcca agtggaccag gctgtacgag gaaatcatca cccgccaggg
     3421 gcggtagcgg cgaaaggaaa accgatgggc aaggaatcgc actacagcag caagaaggac
     3481 cgcgtgttcg tccgggccat cgcgggccag tacaacctca aggacgagct gaagcgcctg
     3541 cgctcgctgc cgcgcgtgat caagggcagg gagctcaagt tccaggacgg gccgcagtcg
     3601 ttcagcaagc acttcgtcga gccggtcgac ggcatgacgc agacgctgca catccacctc
     3661 gaggagtacg cgcccggcgg ccgcacgcag aagcacggcc acgtgaacga ggcggcgttc
     3721 tacatcctcg acgggcgcgg ctacgagatc cacgacggcg tgcgctacga ctggcaggcg
     3781 ggcgacgtcg cgatcgtgca caacaattgc gtgcaccagc accacaacgc cgaccccgag
//
LOCUS       DATDMW010000259         8115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_328121, whole genome shotgun sequence.
ACCESSION   DATDMW010000259 DATDMW010000000
VERSION     DATDMW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328121"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VI319_11170"
     CDS             complement(<1..1187)
                     /locus_tag="VI319_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6864448.1"
                     /translation="MPDRPRILIAGAGIGGLAAAAALLQRGFDVSVFEQAKELKEVGA
                     GVQISANGTRVLDALGVLAPLRRLACEASGKEIRLWNSGRTWKLFDLGAVSVERYGFP
                     YLTVYRPDLLAALADAVRAKKPDAIRLGSRVVGVEQRDDRVVLMLEGGASAEGDVLIG
                     ADGVHSKIREALFGEHDASFTGLMAWRATVPMSKLPAHMNRPVGTNWVGPGRHVVHYP
                     VHGGERMNFVGIVERGDWRVESWTAKGTTEECAADFAGWHEDVHAMIRNAPSLYKWAL
                     LGREPMQRWTDGNVTLLGDACHPMVPMLAQGAVMAIEDGMVLARCLERFEPREALTRY
                     EKARKARTTRAVIGSAQNATRFHNPKLADAAGAQEYVDREWSEARVEERYGWLFTYAA
                     TTT"
     gene            complement(1201..2220)
                     /locus_tag="VI319_11175"
     CDS             complement(1201..2220)
                     /locus_tag="VI319_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864449.1"
                     /translation="MSKKNAFVLAGMLLVAAQFGNEALAQQPIELISFGGGANWPVWT
                     AMDKGLFAKHGVAVKQTFTPNSVFQMENLVAGKFDIATTALDNVVAYMEGQGEVELKT
                     QPDLFAFMGGYAGGLRLDVAPDIKSIADLKGKAVGVDAATTGFAFILYKMLAMNGVKM
                     EDYSIEKLGGTPNRVKALMEGKISATMVSSPSEVLPESKGFHRIADTTKIFGAYQTSV
                     GTTRRAWAKENKERLVGFIRAYVEAIDWLYDPKHKAEALEIYTKHLKDAPKQAAEQSY
                     KIMLSGSEGFQKKAKISMRGARTVLKLRSEYAKPQKDLKDPRKYIDETYYREAVKRPA
                     TKSDT"
     gene            complement(2241..3398)
                     /gene="argE"
                     /locus_tag="VI319_11180"
     CDS             complement(2241..3398)
                     /gene="argE"
                     /locus_tag="VI319_11180"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY6864450.1"
                     /translation="MDTLDILETLIGFDTVSRNSNLGLIEWVRDRLKRLGVVSRLSYD
                     ASRAKANLLATLGDGTTGGLVLCGHTDVVPVDGQPWDSDPFRAVRRDGRIYGRGAADM
                     KGFIACVLAAVPALLEAPLRRPVHLAWTYDEEVGCLGAPVLIRDLRAAGLAPAGCIVG
                     EPTAMQVMIGHKGLRVYRCRVHGLEAHSSLAPSGVNAIEHAARLISRIRDIALELSRE
                     GPRDESFSVPFVTMSTGTIRGGTATNIVPRECEFQFDLRYLPGMRADEVFERVRRAAD
                     AEQAEMRRAAPGVGIALELAEEAPDLNTAPDAALVRLGARLSASGAPGRVPFATDGGH
                     FHRAGVPTIVIGPGSIDQAHKPNEFVALEQLAQCERFLERLKEELCAPAHV"
     gene            complement(3406..4254)
                     /locus_tag="VI319_11185"
     CDS             complement(3406..4254)
                     /locus_tag="VI319_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6864451.1"
                     /translation="MRRLALDFAASRVALAAAALLALLALAALLAPWAAPQNPYDLAQ
                     LDLANSRLAPGARFWLGTDDQGRDMLSAILYGLRTSLTVGAVTTACALALGVALGVTS
                     AWLGGGFEALVMRIADLQLSFPSILIALVLLALLGQGLDKIVAALVAVQWAYYARTAR
                     AAALVERRKDYIEAATALALPGRTIVLRHLLPNCLPPIVIVAGVQTAAAITLEATLSF
                     LGLGLPITEPSLGLLVANGFAYILSGQYWISVFPGAALVVLVVAINLSTDRLREVLDP
                     RSRGLY"
     gene            complement(4251..5222)
                     /locus_tag="VI319_11190"
     CDS             complement(4251..5222)
                     /locus_tag="VI319_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6864452.1"
                     /translation="MLRYLAVRLLQALAALLAMSAIVFAAVYAIGNPVEILVNPQADQ
                     QEIARAVAALGLDRPLPEQYLRFLAHAATGDLGRSFAFDVPAIGLILERMPATLELAT
                     CAMLIALALGLPLGLWAGLRPRSATGRAIAGLSILGFSLPTFWVGLMLILVFSVQLGW
                     LPSAGRGPTIPLLGVPVSFLSLEGWRHLVLPACNLALFNLALVTRLARAGTADAMRQE
                     YVVFARAKGLGEAHIVATHVAKNVLIPIITVAGLEFGSLIAFAVVTESIFAWPGMGKL
                     LVDSINVLDRPVIVGYLCIVVVMFVAINLAIDVAYAWLDPRVRGALA"
     gene            complement(5229..7043)
                     /locus_tag="VI319_11195"
     CDS             complement(5229..7043)
                     /locus_tag="VI319_11195"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864453.1"
                     /translation="MLMQLLRDAFGGARHRRLLEDARRRLDTGDLEGAERLCAAIDEA
                     TPGLADVRLALADALERSGRRDRAAVWRTRALHGLAPGDPRRVGLMLRLADEMEATRP
                     EESEGWYRRVLEADPGNRDALLHVAMRCHDASDPEQARILLERAISPRDTALRLRRAL
                     MLPAIVQSNEHIESIRRRFDAELDELVEARLPPLEHPEREIGMTGFYLSYHGRNNAAP
                     LRKLGRLCRAVYPGRTECARAPFSSGARLRVGFVSTFFHHHSVARTTYGLIRDLPRPR
                     FEVTVFAIEPRADEWADAVRGAADRYVALPGDLARVRDAIDAAALDVLIFADIGMHPL
                     TYFLSFWRMAPVQLATWGHSMTSGVDTVDGYVSSDAVEAPGSEEQYTERLMRLPGYFM
                     PRYRRPALDGPRKPRSALGLPDGVHVYYCPQNLFKIHPDLDASLLAILERDPAAGILL
                     QDTHPAWTALVRKRLARTLGAQAPRVHFVRRVPPGEFLQYLAAADVILDPFYFGGCNS
                     SCEALALGAPVVTLPSGQLPGRFTLGLYREIEMDELVADSPARYVDIAVRLACEPDWR
                     RDVSERIAARSARLFDRPDTGAALGDALLAMAETARRS"
     gene            complement(7054..>8115)
                     /locus_tag="VI319_11200"
     CDS             complement(7054..>8115)
                     /locus_tag="VI319_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864454.1"
                     /translation="LERPDLAVQIFDSHLCVAEVLLYGPTPYDEVLALAAGLRETAEK
                     AGVQRAVGFAAALSGEAALLSGDLDAIEQVPPPDLARVRRDPALHVEQKVSWRTIFFF
                     ADQRERAPGLTDASGAPLARNPLRDRRVREALSKAIDRRAIAERLMDGAALPASNLVS
                     PPVFGYARELQPDPLDPAAARRLLAEAGYPRGFGMTVSAPNNRYVDDDQIAQAVAAML
                     ARVGVRARVETMPINAYLTRGREGRFGFALLGWGSFSADLALRALVATPSPERGFGAF
                     NWAHYANARVDELLERGFGTLDERRREEIAREAMRAAMRDYAVIPLHHQITSWALRRG
                     LSYPGRVDEFTLANFVRRQ"
BASE COUNT         1210 a         2872 c         2920 g         1113 t
ORIGIN      
        1 tccgtcgtcg tcgccgcgta cgtgaacagc catccataac gctcctcgac gcgcgcttca
       61 ctccactcgc ggtcgacata ctcttgcgcg ccggcagcgt cggcgagctt ggggttgtgg
      121 aagcgagtcg cgttctgcgc ggagccgatc acggcgcgtg tcgtgcgcgc ttttcgcgcc
      181 ttctcgtagc gcgtgagcgc ctcccgcggc tcgaagcgct cgaggcagcg tgcgagcacc
      241 atcccgtcct cgatcgccat taccgcgccc tgcgcgagca tcggcaccat cgggtggcag
      301 gcgtcgccga gcagcgtgac gttgccgtcg gtccagcgct gcatgggctc gcggccgagc
      361 agcgcccatt tgtacaagga aggcgcgttg cggatcatcg cgtgcacgtc ctcgtgccag
      421 cccgcgaagt cggcggcgca ctcttccgtc gtgcctttcg cggtccacga ctcgacgcgc
      481 cagtcgccgc gctcgacgat gccgacgaag ttcatccgct cgccgccgtg cacggggtaa
      541 tggacgacgt gacgtcccgg gccgacccag ttggtgccga ccgggcggtt catgtgcgcc
      601 ggcagcttcg acatcggcac ggtcgcgcgc caggccatga ggccggtgaa gctcgcgtcg
      661 tgctcgccga agagcgcctc gcggatcttc gagtgcacgc cgtcggcgcc gatcagaacg
      721 tcgccttcgg cgctcgcgcc gccttcgagc atcaggacga cgcggtcatc gcgctgctcg
      781 acaccgacga cgcgcgaccc gaggcgtatc gcgtcgggct tcttcgctcg caccgcgtcg
      841 gcgagcgccg cgagcaggtc cgggcgatag accgtgagat agggaaaccc gtagcgctcg
      901 accgagaccg cgccgagatc gaacagcttc cacgtgcgcc ccgaattcca gaggcggatc
      961 tccttccccg acgcctcgca cgcgagcctg cgcaaaggcg cgaggacccc gagcgcgtcc
     1021 agcacgcgcg tgccgttggc gctgatctgg acgccggcgc cgacctcctt cagctccttc
     1081 gcctgctcga agaccgagac gtcgaagccg cgctggagca gggcggcggc ggcggcgagg
     1141 ccgccgatgc cggcgcccgc gatcaggatg cgcgggcggt cgggcatctc gcgggcgctc
     1201 tcaggtgtcg ctcttcgtcg cgggccgctt caccgcctcg cggtagtacg tctcgtcgat
     1261 gtacttccgg ggatccttca ggtccttctg cggcttggcg tactcggagc gcagcttgag
     1321 cacggtgcgc gcgccgcgca tgctgatctt cgccttcttc tggaagcctt cgctgcccga
     1381 gagcatgatc ttgtaggatt gctccgcggc ctgcttcggc gcgtccttca ggtgcttcgt
     1441 gtagatctcg agcgcctcgg ccttgtgctt cgggtcgtag agccagtcga tcgcctcgac
     1501 gtaggcgcgg atgaagccga ccaggcgctc cttgttctcc ttcgcccacg cgcgccgcgt
     1561 cgtgccgacg ctggtctggt aggcgccgaa gatcttcgtc gtgtcggcga tgcgatggaa
     1621 gcccttcgac tcgggcagca cttccgaggg cgagctcacc atcgtcgccg agatcttgcc
     1681 ctccatcagc gccttcaccc ggttcggcgt gccgccgagc ttctcgatgc tgtagtcctc
     1741 catcttcacg ccgttcatcg ccagcatctt gtacaggatg aacgcgaagc ccgtggtcgc
     1801 ggcgtcgacg ccgaccgcct tgcccttgag gtcggcgatc gacttgatgt cgggcgcgac
     1861 gtccagcctc aagcccccgg cgtagccgcc catgaacgcg aacaggtcgg gctgcgtctt
     1921 cagctccacc tcgccctggc cctccatgta cgcgacgaca ttgtcgagcg cggtcgtcgc
     1981 gatgtcgaac ttgccggcga ccaggttctc catctggaac accgagttcg gcgtgaacgt
     2041 ctgcttcacc gcgacgccgt gcttcgcgaa caggcccttg tccatggccg tccacaccgg
     2101 ccagttggcg ccgccgccga acgagatcag ctcgatcggc tgctgcgcca gggcctcatt
     2161 tccgaactgc gccgcgacga gcagcatccc ggctaaaacg aacgcattct tcttcgacat
     2221 ctttcctcct cgctgatgga tcaaacgtgc gccggggcgc acagctcctc tttcaggcgc
     2281 tcgagaaagc gctcgcactg cgcgagctgc tcgagcgcga cgaactcgtt cggcttgtgc
     2341 gcctggtcga tggacccggg cccgatcacg atcgtcggca cgcccgcgcg gtggaagtgc
     2401 ccgccgtcgg tggcgaacgg cacccgtccc ggcgcgccgc tggccgacag gcgcgcgccg
     2461 aggcgcacga gcgcggcgtc gggggcggtg ttcagatccg gcgcttcctc ggcgagctcg
     2521 agcgcgatgc ccaccccggg cgcggcgcgg cgcatctcgg cttgctccgc gtcggctgcg
     2581 cggcgcactc gctcgaagac ctcgtccgcc ctcatccctg gaagatagcg caaatcgaac
     2641 tggaactcgc attcgcgcgg cacgatgttg gtcgcggtgc cgccgcggat cgtgcccgtg
     2701 ctcatcgtca cgaagggcac cgagaagctc tcgtcgcgcg gcccctcgcg cgaaagctcg
     2761 agcgcgatgt cgcggatgcg cgagatcagg cgcgccgcgt gctcgatcgc gttcacgccc
     2821 gagggcgcga gcgaggagtg cgcctcgagg ccgtgcacgc ggcagcggta cacgcgcagg
     2881 cccttgtggc cgatcatcac ctgcatcgcg gtcggctcgc cgacgatgca gccggcgggc
     2941 gcgaggcccg cggcgcgcag gtcgcggatc agcaccggcg cgccgaggca gccgacctcc
     3001 tcgtcgtagg tccaggcgag gtgcaccggg cgcctgagcg gcgcctcgag cagcgcgggc
     3061 accgcggcga ggacgcacgc gatgaagccc ttcatgtcgg cggctccgcg cccgtagata
     3121 cggccgtcgc ggcgcacggc gcggaacgga tcgctgtccc agggctggcc gtcgaccggg
     3181 accacgtcgg tgtgcccgca gagcacgagg cccccggtcg tgccgtcgcc cagcgtggcg
     3241 agaaggttcg ccttcgcgcg cgaggcgtcg tacgacaggc gcgacacgac gccgagccgc
     3301 ttcagccggt cgcgcaccca ttcgatcagc ccgagattgg agttgcggct gaccgtgtcg
     3361 aagccgatca gcgtctccag gatgtcgagg gtgtccatgg ccgcttcagt ataagccgcg
     3421 cgagcgcggg tcgagcacct cgcgcaggcg gtcggtcgag aggttgatcg cgaccacgag
     3481 gacgaccagc gccgcgcccg ggaacacgct gatccagtac tgccccgaga gaatgtaggc
     3541 gaagccgttc gcgaccagca gcccgagcga gggctcggtg atcggcaggc ccaacccgag
     3601 gaaggacagc gtcgcctcca gcgtgatcgc cgccgccgtc tggaccccgg cgacgatgac
     3661 gatcggcggc aggcagttcg ggagcaggtg acgcaggacg atcgtgcggc ccggcagcgc
     3721 gagcgcggtc gccgcctcga tgtagtcctt cctgcgctcg accagcgccg cggcgcgcgc
     3781 cgtgcgcgcg taatacgccc actgcaccgc gaccagcgcg gcgacgatct tgtccaggcc
     3841 ctggccgagc agcgcgagca acaccagcgc gatcaggatc gacgggaacg agagctgcag
     3901 gtcggcgatg cgcatcacga gcgcctcgaa ccccccgccc agccacgcgg aggtcacgcc
     3961 gagcgcaacg ccgagcgcga gcgcgcacgc cgtcgtcacc gcgccgaccg tgaggctggt
     4021 gcgcaggccg tagaggatgg ccgagagcat gtcgcggccc tggtcgtcgg tcccgagcca
     4081 gaagcgcgcg cccggagcca ggcgcgagtt cgcgaggtcg agctgcgcga gatcgtacgg
     4141 attctgcggc gcggcccagg gcgcgagcag cgccgcgagc gcgagcagcg cgagcagggc
     4201 cgcggcggcc agcgcgacgc ggctcgcggc gaagtcgagc gcgagccgcc tcacgcgagc
     4261 gctccgcgca cgcgcgggtc gagccacgcg taggcgacgt cgatcgcgag gttgatcgcg
     4321 acgaacatca cgaccacgat gcacagatag ccgacgatca ccggccggtc gagcacgttg
     4381 atcgagtcga ccagcagctt gcccatcccg ggccaggcga agatcgactc ggtgacgacg
     4441 gcgaaggcga tcagcgagcc gaactcgagc cccgcgaccg tgatgatggg aatgagcacg
     4501 ttcttggcga cgtgcgtggc gacgatgtgc gcctcgccga gccccttcgc gcgggcgaac
     4561 accacgtact cctggcgcat cgcgtcggcc gtgcccgcgc gagcgagccg cgtcacgagc
     4621 gcgagattga acagcgcgag attgcacgcg ggcagcacga ggtggcgcca gccctcgagc
     4681 gacaggaagc tgaccggcac gccgagcagg gggatcgtcg gcccgcgccc cgccgacggc
     4741 agccagccga gctgcaccga gaagaccagg atcagcatca gcccgaccca gaacgtcggc
     4801 agcgagaagc cgaggatcga aagcccggcg atggcgcgcc ccgtcgccga gcgcggccgc
     4861 agtcccgccc agagcccgag cggcaggccg agcgcgagcg cgatcagcat ggcgcaggtc
     4921 gcgagctcca gcgtcgccgg catgcgctcg aggatgagcc cgatcgcggg cacgtcgaag
     4981 gcgaacgagc gcccgaggtc gcccgtcgct gcgtgcgcga ggaagcgcag gtactgctcg
     5041 ggcagcggcc ggtcgaggcc gagcgccgcg accgcgcgcg cgatctcttg ctggtcggcc
     5101 tgcgggttca ccaggatctc gaccgggttg ccgatcgcgt agaccgcggc gaacacgatc
     5161 gccgacatcg cgagcagcgc ggcgagcgct tgcagcagcc gcaccgcgag atagcgcagc
     5221 acggcgcgct agctccggcg tgccgtctcg gccatcgcga gcagcgcgtc gccgagcgcg
     5281 gcgccggtgt ccggccggtc gaacaggcgc gcgctgcgcg cggcgatgcg ctccgacacg
     5341 tcgcggcgcc agtcgggctc gcacgcgagc cgcaccgcga tgtcgacgta gcgcgcgggg
     5401 ctgtcggcga cgagctcgtc catttcgatc tcgcggtaca ggcccagcgt gaagcgcccg
     5461 ggcagctgcc ccgagggcag cgtcaccacc ggcgcgccga gcgccagggc ctcgcagctc
     5521 gagttgcatc cgccgaagta gaaggggtcg aggatgacgt cggcggcggc gaggtactgg
     5581 aggaattcgc ccggcggcac gcggcgcacg aaatgcacgc gcggcgcctg cgcgccgagc
     5641 gtgcgcgcga ggcgcttgcg cacgagcgcg gtccacgccg ggtgcgtgtc ctgcagcagg
     5701 atgccggccg cgggatcgcg ctcgaggatc gcgagcagcg acgcgtccag gtccggatgg
     5761 atcttgaaca ggttctgcgg acagtagtag acgtggacgc cgtcgggcag gccgagcgct
     5821 gagcgcggct tgcgcgggcc gtccagcgcg ggccgccggt agcgcggcat gaaatagccg
     5881 ggcaggcgca tcaggcgctc ggtgtactgc tcctcgctcc ccggcgcctc gacggcgtcg
     5941 gaggagacgt acccgtcgac ggtgtcgacg ccgctcgtca tcgaatgtcc ccaggtcgcg
     6001 agctgcaccg gcgccatgcg ccagaacgac aggaaatacg tgagcgggtg catgccgatg
     6061 tccgcgaaga tcagcacgtc gagcgcggcg gcgtcgatcg cgtcgcgcac gcgcgccagg
     6121 tcgcccggca gcgcgacgta gcggtcggcg gcgccgcgca cggcgtcggc ccattcgtcg
     6181 gcgcgcggct cgatcgcgaa caccgtcacc tcgaaccgcg gccgcggcag atcgcggatc
     6241 aatccgtagg tggtgcgagc gacggaatga tgatggaaga aggtcgagac gaagccgacg
     6301 cgcaggcgag cgccggagga gaacggcgct cgcgcgcact cggtgcggcc cgggtagacc
     6361 gcgcggcaca ggcggccgag cttgcgcagc ggcgcggcat tgttgcggcc gtggtacgag
     6421 aggtagaacc cggtcatccc gatctcgcgc tcggggtgct cgagcggagg cagcctggct
     6481 tcgaccaact cgtcgagctc cgcgtcgaag cgacgccgga tcgactcgat gtgctcgttc
     6541 gactgcacga tcgccggcag catgagcgcc cggcgcaggc gcagcgcggt gtcgcgcggg
     6601 gagatcgcgc gctcgagaag gatgcgcgcc tgctcggggt cggacgcgtc gtggcagcgc
     6661 atcgcgacgt gcagcagcgc atcccggttg ccgggatcgg cctcgaggac gcggcggtac
     6721 cagccctcgg actcctcggg gcgcgtcgcc tccatctcgt cggcgaggcg cagcatcaga
     6781 ccgacacggc gcggatcccc gggggcgagc ccgtgcagcg cgcgagtcct ccacaccgcc
     6841 gcgcggtcgc gccgcccgga gcgctcgagc gcgtcggcga gcgcgaggcg aacgtcggcg
     6901 aggcccgggg tcgcctcgtc gatcgccgcg cacagccgct cggcgccttc caggtcgcct
     6961 gtgtcgagtc ggcggcgcgc gtcctcgagc agccgcctgt ggcgcgctcc accgaacgcg
     7021 tcgcgcagca gttgcatcag catggatgcg atcctattgc ctgcgcacga aattggccag
     7081 cgtgaactcg tcgacgcggc ccgggtacga gagcccgcgc cgcagcgccc agctcgtgat
     7141 ctggtggtgc agggggatga ccgcgtagtc gcgcatcgcc gcgcgcatcg cctcgcgggc
     7201 gatctcctcg cggcggcgct cgtcgagcgt cccgaagccg cgctcgagca gctcgtcgac
     7261 gcgcgcgttc gcgtagtgcg cccagttgaa cgcgccgaag ccgcgctcgg gcgaaggcgt
     7321 cgcgaccagc gcgcgcagcg cgaggtcggc cgagaacgat ccccacccga gcagcgcgaa
     7381 gccgaaccgc ccttcgcgcc cgcgcgtgag gtaggcgttg atgggcatgg tctcgacgcg
     7441 cgcgcgcacg ccgacgcgcg ccagcatcgc cgccaccgcc tgcgcgatct ggtcgtcgtc
     7501 gacgtatcgg ttgttcgggg cggacaccgt catgccgaat ccgcgcgggt agcccgcctc
     7561 ggcgagcagc cggcgcgcgg cggcgggatc gagcggatcg ggctgcagct cccgcgcgta
     7621 gccgaacacg ggcggcgaga cgagattcga cgcggggagc gccgcgccgt ccatcaggcg
     7681 ctcggcgatg gcgcgccggt cgatcgcctt cgacagcgcc tcgcgcacgc gccggtcgcg
     7741 cagcgggttg cgcgcgagcg gcgcgcccga ggcgtcggtg agtccggggg cgcgctcgcg
     7801 ctggtcggcg aagaagaaga tcgtgcgcca cgacaccttc tgctcgacgt ggagcgccgg
     7861 gtcgcggcgc acgcgcgcga gatcgggcgg cggcacctgc tcgatcgcgt cgaggtcgcc
     7921 cgagagcagc gccgcctcgc cggagagcgc ggccgcgaag cccacggcac gctgcacgcc
     7981 ggccttctcg gccgtctccc gcaggccggc ggccagcgcg agcacctcgt cgtagggggt
     8041 gggtccgtag agcagcacct cggcgacgca tagatgggag tcgaagatct gcaccgccag
     8101 atccggccgc tccag
//
LOCUS       DATDMW010000260         8903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1324561, whole genome shotgun sequence.
ACCESSION   DATDMW010000260 DATDMW010000000
VERSION     DATDMW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324561"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1744
                     /locus_tag="VI319_11205"
     CDS             86..1744
                     /locus_tag="VI319_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEY6864455.1"
                     /translation="MWNQVYNPFSNELISTLVAAIPVVTLLALIASGKVKAHIAAVIA
                     LAAAIVVAVYAFTMPAAMAFRAGVLGLVTGFFPIGWIVLNVIFLYRLTVEKGAFQILQ
                     QTIGGVTADRRLQLLLIAFSFGAFFEGASGFGTPVAVTGAILIGLGFSPLAASGLSLI
                     ANTAPVAYGALGTPIAGLASVTGIDPYLLGAMVGRQLPFFSLIVPFWLIWVFAGWRGM
                     KEIWPAILVTGVTFAVPQYLISNFVNPWIVDIGASLISMACLIGFLRIWQPKTVWTSP
                     KLRTHDDSAATMAAPAPAPAGKASSAEVWKALTPWIIVCAILLVWGTGWFKSMVNPHF
                     TWNYPVPGLHNLINKVQPVVAKPTPEGAVFAFTWLSFTGTGMLIAAIISGFFMGYSPM
                     GLIKSYGRTIYVTRYSLITISAMLGIGTLTRLSGLDATLGLAFAHAGVLYPFFGALLG
                     WLGVALTGSDTASNVLFGGLQKITSEQLGLSPILMCAANSSGGVMGKMIDAQSIVVAS
                     TATNWFGHEGSILRFVFWHSIALACLVGILVTLQAYVFTGMIVH"
     gene            complement(1791..2606)
                     /locus_tag="VI319_11210"
     CDS             complement(1791..2606)
                     /locus_tag="VI319_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864456.1"
                     /translation="MTLSLAPLSALADRSSRENEGVPFRLLQQRIDALRLRVDALEAA
                     RPTAQSCQAGQFVAAVTATGTIVCAAPPTTTTGGGGTTAGGGTTTPPGPVSDPFRAAL
                     QQAFSALSGANAPLQPRLIDLIGAQPAATIPTSYDLTIGAAVFAQTSATAGTITVAVP
                     SFVVHANYSNDPNGVVIGGTLAFSAGPGTTANIDVTVETTPTGKRRLGSVTSIQVNIP
                     QVGVNAPIGVTGSDLLVVSNIVFLTQYAKAITSEYVGILGTLVPPALATLPEF"
     gene            complement(2662..3699)
                     /locus_tag="VI319_11215"
     CDS             complement(2662..3699)
                     /locus_tag="VI319_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864457.1"
                     /translation="MKYARRVGPGCGARNSRLNFARSRPEKPRSSHAPVGPPRPCSDA
                     VATEPTCEGTTMKNRSPWWLCVLALSLVPFSAEADQRGDDDGVPFRLLQQQLDALTQR
                     VGALEAARQTTGQSCQAGQFVAAISATGVITCAAPATAGGAGGAGGTTAGGTTTGGTT
                     AGGGTGAGGANGTTPPPPVVSDAFRAAVQQAFSALSANNVPLAPTPLVIPFTSVIPTS
                     YDLAVGTVVLAIDAPSATATITVAIPTFVWRADASNSLLGAGVLTVTASPATTATIQA
                     AVETTPTGTHRIGTVMSIAVTNAAVTATGAGGLSTALQNMVPFPAILVDEYSSILGGI
                     VPAALATLPEF"
     gene            3929..5335
                     /locus_tag="VI319_11220"
     CDS             3929..5335
                     /locus_tag="VI319_11220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6864458.1"
                     /translation="MASRCIVLSHRDEDPLAASARACLEQVPALEIAVQRVPAAGEPV
                     PDADVLVVPAEPATLQRTLDLLGRVRLESPGTSVLVLGAGFAQEDILRILTAGAYDFV
                     SAPFLVAELVARTLRGVGLLQPRPPRNIRALLGARAEGLMGNSPAFLAHMARLPLLAG
                     CDAGVLILGETGTGKELFAQAIHYLSARVSRPCVPINCGAIPVELIEAELFGHARGAY
                     TTAHAAREGLIAAAEGGTLFLDEVDSLPHAAQAKLLRFLQEREYRPVGANALRRANVR
                     VIASSNQDLAGLVAHGRFRRDLYFRLNVLTLTLSPLRERREDIPALAQHFVRRFATEF
                     GRPVQGVTPLAMKKLLAHSWPGNVRELSHTIERAVLLGHGPSLTPAEIEIGDEEQAPL
                     DESFRAAKARVVEQFERRYIEQMLIACDGNITHAAAAAKKHRRALWELIRKHRIDPKR
                     FRREPAEPAADRPFNVSF"
     gene            5348..5680
                     /locus_tag="VI319_11225"
     CDS             5348..5680
                     /locus_tag="VI319_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864459.1"
                     /translation="MAKRRTKAGKPKSRPAKKVSAKKAAPAKAAAPAGASHLHQVIEE
                     LISAKQRGDHAAGEKAVSALKALRKGHAAAPVRQAAAEACAVADNPVLMESTRMLGNI
                     AARVRAHA"
     gene            complement(5685..8516)
                     /locus_tag="VI319_11230"
     CDS             complement(5685..8516)
                     /locus_tag="VI319_11230"
                     /inference="COORDINATES: protein motif:HMM:NF025298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEY6864460.1"
                     /translation="MVDSGELADANKEKKGLEQKAKTEPAAIQDFARQETARVDRELA
                     HEEKAGRDAMGAKRRAALASTGQRQHGAKSALEKQRDDVAAQINGMYKKAQDSVTKKL
                     ADLERDSMKRFDEGNAKAAKTFEDTVNREFEAYKADRYSGWFGWARKAKDWLLGMDEL
                     PRVKEIFDTNRAIFVETVNQLVAAITADNKRVIQQCKDELADARKQIADFVKGLKPAL
                     QGIGKKAAAEMSEKLADLDNKVGAKEEELRGKLKDKQTAAIRAIDDKITKMKEAMSGA
                     LAKLGRLLLYAAKKFFSWALGKFGVSLSTIEGIIDKGIAVLKAIFTGPIKFVKNLVGA
                     AGLGFENFGKHFLTHLKNAVFEWLTGSLEGITLPETWNLRGILSVVLQIAGITWENLR
                     KHLLKYIPEPVLKGLETGFALVKTLVTEGPMAAWEQLKSIGAELTDAFVDAVKNWIKW
                     KIVQKAIETILSMFIPGAGIIRAIIGIYDTIVFFIQKAKQIMEMIGNFLGSIADIAAG
                     NIGAAAQALEDGLANGLKLVIQFLAKFLHLDGITAKIREVIGGIRNKVDGVLDKVASW
                     VAGLAKKAGRFVASKALGGDPKATPEQRLQAGVSEGVQAVNRLGGREIGITLINPVLA
                     AIRLRHNLKSLQAQPSGGVWSVVGEVNPKLAALTEKAVLTDKKTPGATKREYGSLTGG
                     GFGTWGRVTGLHKLGAENPPSVDNAKHWQVLSRRLFRRGGSTYYVRGHLINGKFGGPG
                     DDWHNLTPLTQASNNRSIASMLHTFENPVKQAVESNGTVDVDVRAVYKQPDRSGDIAK
                     VLGKRPSAEDKAIAGVIEAEQHVPSQIVATAHIVSGGGGAKDPVSTTENVIDTNLDNY
                     FHADYPSAGGPARRLVDLNKATRSELKELVGMTDAVATAIISRRRYSDRDGLIAAMDD
                     DQVVKVTHMSGAQVWHNMMSTAGVKLVFE"
     gene            complement(8526..>8903)
                     /locus_tag="VI319_11235"
     CDS             complement(8526..>8903)
                     /locus_tag="VI319_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864461.1"
                     /translation="APVVEPGKAEGRLTESIQENIPKTLEDVDNFRKDQRGRHMSADV
                     LEVVQGDKNSVVTTFGDVRTTPPPVPSGHVAVELPPAEAAPHTPPMNLGKGAVAPLEK
                     EHTDTSSYTSQADAKLKEEGVSQ"
BASE COUNT         1192 a         3086 c         3076 g         1549 t
ORIGIN      
        1 gcgcggaccc cgagtcagca ttctgtaaga gaccgggctc tatcctccgc gcggcacgac
       61 caaaaccaca accggagggg acgcgatgtg gaaccaggtc tataacccgt tctcgaacga
      121 gctaatttcg acgctggtcg ccgcgatccc ggtggtgacg ctgctcgcgc tgatcgcctc
      181 cggcaaggtc aaggcgcaca tcgccgccgt gatcgcgctc gccgcggcga tcgtcgtcgc
      241 ggtgtacgcc ttcaccatgc ccgcggccat ggcgttccgc gccggcgtcc tcggtctcgt
      301 gaccggcttc ttcccgatcg gctggatcgt gctcaacgtc atcttcctgt accggctcac
      361 ggtggagaag ggcgcgttcc agatcctgca gcagacgatc ggcggcgtca ccgcggaccg
      421 ccgcctgcag ctcctgctca tcgccttctc cttcggcgcc ttcttcgaag gcgcctcggg
      481 cttcggcacg ccggtcgcgg tgaccggcgc gatcctcatc ggcctcggct tctcgccgct
      541 cgccgcgtcg ggcctgtcgc tgatcgcgaa caccgcgccg gtcgcctacg gcgcgctcgg
      601 cacgccgatc gccggtctcg cgtcggtgac cggcatcgac ccgtacctgc tcggcgcgat
      661 ggtcgggcgc cagctgccgt tcttctcgct gatcgttccg ttctggctga tctgggtctt
      721 cgccggctgg cgcggcatga aggagatctg gcccgcgatc ctggtcaccg gcgtcacgtt
      781 cgcggtgccg cagtacctga tctcgaactt cgtcaacccg tggatcgtcg acatcggcgc
      841 ctcgctcatc tcgatggcgt gcctgatcgg cttcctcagg atctggcagc cgaagacggt
      901 gtggacctcg cccaagctgc gcacgcacga cgactcggcg gcgacgatgg cggcgccggc
      961 gcccgcgccc gcggggaagg catcctcggc cgaggtgtgg aaggcgctca cgccgtggat
     1021 catcgtctgc gccatcctcc tggtctgggg caccggatgg tttaagtcca tggtcaaccc
     1081 gcacttcacc tggaactacc cggtccccgg cctgcacaac ctgatcaaca aggtgcagcc
     1141 ggtcgtggcg aagccgacgc ccgagggcgc ggtgttcgcg ttcacctggc tctcgttcac
     1201 cggcaccggc atgctgatcg ccgcgatcat ctcgggcttc ttcatgggct attcgccgat
     1261 ggggctgatc aaatcatacg gccgcacgat ctacgtgacg cgctactcgc tgatcacgat
     1321 ctcggccatg ctcggcatcg gcacgctgac gcgcctctcc ggcctcgacg ccacgctcgg
     1381 cctcgccttc gcgcacgcgg gcgtgctgta cccgttcttc ggcgcgctgc tcggctggct
     1441 gggcgtcgcg ctcacgggct ccgacaccgc gtcgaacgtg ctcttcggcg gcctgcagaa
     1501 gatcacctcc gagcagctcg gcctctcgcc gatcctcatg tgcgcggcca actcctcggg
     1561 cggcgtcatg ggcaagatga tcgacgcgca gtcgatcgtg gtcgcctcga ccgcgaccaa
     1621 ctggttcggc cacgagggct cgatcctgcg cttcgtcttc tggcactcga tcgcgctcgc
     1681 gtgcctggtc ggcatcctgg tgacgctgca ggcgtacgtg ttcacgggga tgatcgtgca
     1741 ctgacgctcg gggcgcctcg aaggcgcccc ggtcccgctt cacccggcga tcagaactcc
     1801 ggcagcgtgg cgagcgccgg cgggacgagc gtgccgagga tgccgacgta ttcgctggtg
     1861 atggccttgg cgtactgggt caggaacacg atgttcgaga cgacaagcag gtcgctcccg
     1921 gtaaccccga tcggcgcgtt caccccgacc tgagggatgt tgacctggat cgaggtcacg
     1981 gacccgagcc ggcgttttcc ggtgggcgtg gtctccaccg tgacatcgat gttggcggtc
     2041 gtgcccgggc ccgcggagaa ggcgagcgtg ccgcctatga cgacaccgtt cgggtcgttg
     2101 gaatagttcg cgtgcacgac gaagctcgga accgccaccg tgatcgtccc ggccgtggcc
     2161 gaggtctgcg cgaacaccgc ggccccgatc gtcagatcgt aggaggtcgg gatcgtggcc
     2221 gcgggttgcg ctccgatcaa atcgatgagc cgcggctgaa gcggagcgtt ggcgcccgac
     2281 agcgcgctga aggcctgctg cagggcggcg cggaaggggt cgctgacggg gcccgggggc
     2341 gtcgtcgtgc cgcctcccgc cgtcgtgccg ccgccgccgg tggtcgtcgt cggcggcgcg
     2401 gcgcaaacga tcgtgccggt cgcggtcacg gccgccacga actggccggc ctgacacgac
     2461 tgggcggtcg ggcgcgccgc ctcgagcgca tcgacgcgca ggcgtagggc atcgatccgc
     2521 tgctgcagca gccggaacgg gacgccttcg ttctcgcgcg agctcctgtc cgccagcgcg
     2581 gacagcggcg cgagcgacag cgtcacgagg cacagcaacc acaacgagct tcggttcttc
     2641 attttttgtt tcctcgctcg atcagaactc gggcagcgtg gcgagcgccg ccgggacgat
     2701 gccgccgagg atgctgctgt actcgtcgac gaggatggcc gggaacggga ccatattctg
     2761 gagggcggtg gagaggccgc cggccccggt cgccgtaacc gcggcattgg tgaccgcgat
     2821 cgacatcacg gtgccgatac ggtgcgtgcc ggtcggcgtg gtctccacgg cggcctggat
     2881 cgtggccgtc gtggcggggc tcgccgttac cgtcaatacg ccggcgccga gcaagctatt
     2941 gctcgcgtcg gcacgccaca cgaaggtcgg aatcgcgacc gtgatcgtcg cggtcgccga
     3001 cggcgcgtct atcgcgagca cgacggtccc gacggcgaga tcgtaggagg tcgggatcac
     3061 gctggtgaac ggtatgacca gcggcgtcgg cgcgagcgga acgttgttcg cggagagcgc
     3121 gctgaacgcc tgctgcaccg cggcgcggaa ggcgtcgctg accacgggcg gcggcggtgt
     3181 cgttccattg gccccgccgg ctccggttcc gccgcctgcc gttgttccac cggtggtcgt
     3241 tccgccagcg gtcgttccac cggctccgcc cgcgccgccg gcggttgccg gcgccgcgca
     3301 cgtgatcacg ccggtcgcgc tgatggcggc gacgaactgt ccggcctggc acgactggcc
     3361 ggtcgtctgg cgcgccgcct cgagcgcgcc gacgcgctgg gtgagcgcgt cgagctgctg
     3421 ctgcagcagt cggaacggga cgccgtcgtc gtcgcctctc tgatcggcct cggccgagaa
     3481 gggcacgagc gacagggcca ggacgcacaa ccaccacggg cttcggttct tcattgttgt
     3541 tccctcgcaa gtcggttcgg tcgccacggc atcagagcac ggccgcgggg gcccgacggg
     3601 cgcgtgcgac gaacgaggct tttccgggcg cgaacgcgcg aaattcaggc gtgagttgcg
     3661 ggcgccgcat cccgggccga cgcgccgcgc gtacttcaag aaacgcgtct aacggattga
     3721 tcgcgctcgt gccgagcgcg cggctccgga aactcccggc agcatttgtc gggaattccc
     3781 gatgccgcgc gcgcttgact tggcgcggcc ggtcgctaat atcaaggacg atgctcttgt
     3841 catgagagcg gcccgcgggt ccgcgggcgc aatagtctcc gtcgtcaaaa aaaagcggcc
     3901 cggccaataa caagcggggg gcacggcgtt ggcatcgcgt tgcatcgttc tgtctcaccg
     3961 agacgaggat ccgctcgccg cgagcgcgcg cgcctgcctc gagcaggtgc cggcgctcga
     4021 gatcgcggtg cagcgcgtgc ccgcggccgg cgagccggtt cccgacgccg acgtgctggt
     4081 ggtccccgcg gagccggcga ccctgcagcg cacgctcgat cttctcgggc gcgtgcgcct
     4141 ggagtcgccc gggacgtcgg tgctcgtgct cggcgcgggc ttcgcgcagg aggacatcct
     4201 ccggatcctg accgcgggcg cgtacgactt cgtgtccgcg ccgttcctcg tcgccgagct
     4261 ggtcgcgcgc acgctgcgcg gggtggggct gttgcagccg cgccccccgc ggaacatccg
     4321 tgcgctgctc ggcgcgcgcg ccgagggcct gatgggcaac agcccggcct tcctcgcgca
     4381 catggcgcgg ctgccgctgc tcgcgggctg cgacgcgggc gtgctgatcc tcggcgagac
     4441 cggcaccggc aaggagctgt tcgcgcaggc gatccactac ctctcggcgc gcgtgtcgcg
     4501 cccgtgcgtc ccgatcaact gcggcgcgat ccccgtggag ctgatcgagg ccgagctctt
     4561 cgggcacgcg cgcggcgcgt acacgacggc gcacgcggcg cgcgaaggcc tgatcgcggc
     4621 ggccgaaggc gggacgctgt tcctcgacga ggtcgacagc ctgccgcacg cggcgcaggc
     4681 gaagctgctg cgcttcctgc aggagcgcga gtaccgcccc gtgggcgcga acgcgctgcg
     4741 gcgcgcgaac gtgcgcgtga tcgcgtcgtc caaccaggac ctggcgggcc tggtcgcgca
     4801 cggccggttc cgccgcgacc tgtacttccg gctgaacgtg ctgacgctga cgctttcccc
     4861 cttgcgcgag cggcgcgagg acatcccggc gctcgcgcag cacttcgtgc gccgcttcgc
     4921 caccgagttc gggcggccgg tgcagggcgt gacgccgctc gccatgaaga agctgctcgc
     4981 gcattcgtgg ccgggcaacg tccgcgagct ctcgcacacc atcgagcgcg ccgtgctgct
     5041 cggccacggc ccgagcctca cgccggccga gatcgagatc ggcgacgagg agcaagcgcc
     5101 gctcgacgag tcgttccgcg cggccaaggc gcgcgtcgtc gagcagttcg agcggcgcta
     5161 catcgagcag atgctgatcg cgtgcgacgg caacatcacg cacgcggcgg ccgcggcgaa
     5221 gaagcaccgc cgcgcgctgt gggagctgat ccgcaagcac cgcatcgacc cgaagcggtt
     5281 ccgccgcgag ccggccgagc cggcggcgga ccgtccgttc aacgtctcat tctgaagcga
     5341 ggacaccatg gcgaagcgtc gcacgaaggc aggcaaaccg aagagcaggc ccgcgaagaa
     5401 ggtctccgcg aaaaaggccg cgccggccaa ggccgcggcg cccgcgggcg cgtcgcacct
     5461 gcaccaggtg atcgaagagc tcatctccgc caagcagcgc ggcgaccacg ccgcgggcga
     5521 gaaggccgtg tccgcgctca aggcgctgcg caagggccac gccgccgcgc ccgtgcgcca
     5581 ggcggccgcc gaggcctgcg ccgtcgccga caacccggtg ctgatggaga gcacgcgcat
     5641 gctggggaac atcgcggcgc gggtgcgggc gcacgcgtag ccatttattc gaagaccagc
     5701 ttcacgccgg cggtgctcat catgttgtgc cacacctggg cgccggacat gtgcgtgacc
     5761 ttgacgacct gatcgtcgtc catggccgcg atgagaccgt cgcggtccga ataccgccgc
     5821 cggctgatga tggcggtcgc gaccgcgtcg gtcatcccga ccagctcctt gagctcggac
     5881 cgggtcgcct tgttgaggtc gacgaggcgg cgcgcggggc cgccggcgga gggatagtcg
     5941 gcgtggaagt agttgtcgag gttcgtgtcg atcacgttct cggtcgtgct gaccgggtcc
     6001 ttggccccgc cgccgccgga cacgatgtgc gcggtggcga cgatctggct cgggacgtgc
     6061 tgctcggcct cgatgacgcc ggcgatggcc ttgtcctcgg ctgacggccg ctttcccagc
     6121 accttggcga tgtcgcccga gcggtcgggc tgcttgtaga cggcgcggac gtcgacgtcc
     6181 accgttccgt tcgattccac cgcctgcttc accgggttct cgaaggtgtg cagcatcgag
     6241 gcgatgctgc ggttgttcga ggcctgggtg agcggcgtga ggttgtgcca gtcgtcgccc
     6301 gggccgccga acttgccgtt gatcaggtgg ccgcgcacgt agtaggtgct gcccccgcgg
     6361 cggaacaggc gccgcgacag cacctgccag tgcttcgcgt tgtcgacgct cgggggattc
     6421 tcggcgccga gcttgtgcag tcccgtcacg cgcccccagg ttccgaagcc gccgccggtc
     6481 aggctgccgt actcgcgctt ggtggcgccc ggcgtcttct tgtcggtgag caccgctttc
     6541 tcggtgagcg cggcgagctt cgggttgacc tcgccgacca ccgaccacac accgccgctc
     6601 ggctgcgcct gcaggctttt caggttgtgg cggagccgga tggcggcgag gaccgggttg
     6661 atcagcgtga tcccgatctc ccggccgccc agccggttga cggcctgcac gccttcgctc
     6721 acgccggcct gcaggcgctg ctcgggcgtc gccttcgggt cgccgccgag cgccttgctc
     6781 gcgacgaacc ggcccgcctt tttcgccagc cccgcgaccc agctcgcgac cttgtcgagc
     6841 acgccgtcga ccttgttgcg gatgcccccg atcacctcgc ggatcttggc ggtgatgccg
     6901 tcgaggtgca ggaacttcgc gaggaactgg atgacgagct tcaggccgtt cgcgaggccg
     6961 tcctcgagcg cctgcgccgc ggcgccgatg ttccccgccg cgatgtcggc gatcgagccg
     7021 aggaagttgc cgatcatctc catgatctgc ttcgccttct ggatgaagaa gacgatggtg
     7081 tcgtagatgc cgatgatggc gcggatgatg cccgcgccgg ggatgaacat cgacaggatg
     7141 gtctcgatcg ccttctgcac gatcttccac ttgatccagt tcttgaccgc gtcgacgaag
     7201 gcgtcggtga gctcggctcc gatcgacttc agctgctccc aggcggccat cgggccctcg
     7261 gtgaccagcg tcttcaccag cgcgaagccg gtctcgagcc ccttgagcac gggctcgggg
     7321 atgtacttca gcaggtgctt gcgcaggttc tcccaggtga tgccggcgat ctgcagcacg
     7381 acgctcagga tgccgcgcag gttccaggtc tccggcagcg tgatcccttc gagcgatccg
     7441 gtgagccact cgaacaccgc gttcttcagg tgcgtgagga aatgcttgcc gaagttctcg
     7501 aagcccaggc cggcggcgcc gaccaggttc ttcacgaact tgatcggccc ggtgaagatc
     7561 gccttgagca cggcgatgcc cttgtcgatg atgccctcga tggtcgagag cgacacgccg
     7621 aacttcccga gcgcccagga gaagaacttc ttcgcggcgt agaggagcag ccggccgagc
     7681 ttcgcgagcg cgcccgacat cgcctccttc atcttcgtga tcttgtcgtc gatggcgcgg
     7741 atcgcggcgg tctgcttgtc cttgagcttg ccgcgcagct cctcctcctt cgcgccgacc
     7801 ttgttgtcga ggtcggcgag cttctcgctc atctccgcgg cggccttctt gccgatgcct
     7861 tgcagcgccg gcttcaggcc cttgacgaag tcggcgatct gcttgcgcgc gtcggcgagc
     7921 tcgtccttgc attgctggat cacgcgcttg ttgtcggcgg tgatcgccgc gacgagctgg
     7981 ttcaccgtct cgacgaagat cgcgcggttg gtgtcgaaga tctccttcac gcgcggcagc
     8041 tcgtccatgc cgaggagcca gtccttggcc ttcctcgccc agccgaacca gcccgagtag
     8101 cggtcggcct tgtaggcctc gaactcgcgg ttgaccgtgt cctcgaaggt cttcgccgcc
     8161 ttcgcgttgc cctcgtcgaa gcgcttcatc gagtcgcgct cgaggtcggc gagcttcttg
     8221 gtgacgctgt cctgcgcctt cttgtacatg ccgttgatct gcgccgcgac gtcgtcgcgc
     8281 tgcttctcga gcgcgctctt cgcgccgtgc tggcgctggc ccgtggaggc gagggcggcg
     8341 cggcgcttcg cgcccatcgc gtcgcggccc gccttctcct cgtgcgcgag ctcgcggtcg
     8401 acgcgcgcgg tctcctggcg cgcgaagtcc tggatcgcgg cgggctccgt cttcgccttc
     8461 tgctcgaggc ccttcttctc cttgttggcg tcggcgagct cgcccgagtc gaccatgtcg
     8521 agctgctact gcgagacgcc ttcctccttc agcttcgcgt cggcctggct ggtgtagctg
     8581 ctcgtgtcgg tgtgctcctt ctcgagcggc gcgaccgcgc ccttgccgag attcatcggc
     8641 ggcgtgtgcg gcgccgcctc ggccggcggc agctcgaccg cgacgtggcc cgagggcacg
     8701 ggcggcggcg tcgtgcgcac gtcgccgaag gtcgtgacca ccgagttctt gtcgccctga
     8761 acgacctcga gcacgtcggc gctcatgtgc cggccgcgct ggtccttgcg gaagttgtcg
     8821 acgtcctcga gcgtcttcgg gatgttctcc tggatcgact cggtgagccg gccttcggcc
     8881 ttgcccggct cgacgacggg cgc
//
LOCUS       DATDMW010000261         7054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1200827, whole genome shotgun sequence.
ACCESSION   DATDMW010000261 DATDMW010000000
VERSION     DATDMW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200827"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VI319_11240"
     CDS             complement(<1..718)
                     /locus_tag="VI319_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864462.1"
                     /translation="MIPATAAAAPRKPLARPERWRFSLSDPAGATPLEFPRRGAGLAA
                     VIVAVMFVVFAGAGVLAAKGLDAHPMHSVGALMQTLFRLFWLLGWSLAVLVLGALTVL
                     LCFYRPMTWLSSGALIDAPRIGPLRMIAEYDLARMRNVRAEPEGDNARVRFDYGEGGR
                     TLGDPMPPADAERIVAAIRGAMPASTAAAPPELVIPSAPVPAKPAEAEPPRPLPAVPT
                     LALVLANFLPLLGVLLGGWRL"
     gene            complement(772..1875)
                     /locus_tag="VI319_11245"
     CDS             complement(772..1875)
                     /locus_tag="VI319_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY6864463.1"
                     /translation="MALKRPQGSPGTLVVLEHDSRVLRGNPLGDPHVRRLGVWLPPQY
                     DDGTRRRFPVLYDLVGFTGSGLAHTNWKPFGDNVPERVARLIRHQRMGPAILVFPDCF
                     TALGGNQYVNSSAIGDYADYLTREIVPFVDREFRTLASREHRGCFGKSSGGYGAIVHG
                     MKYARTWGAVANHSGDAYFEFVYGHDWPNTLDELAKHRAPRRRAGRYDALRESRRKGL
                     AAGEDDGRIRRFLAHVWKKEKLSGKEGHCIMNLCMAATYDPDPRAPNGFRVPFNLESG
                     EPVPGRWASWRRHDPVNLVERHRASLRSLRGIYLDCGWRDQYHIHYGMRILSARLARA
                     GIRHTYEEFDDDHSDVDYRMDVSLPFLYRALAP"
     gene            2146..2379
                     /locus_tag="VI319_11250"
     CDS             2146..2379
                     /locus_tag="VI319_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864464.1"
                     /translation="MKMIESIKGWIGALTDVGLMLLALAIVAALLVGGSLPFFGSVVG
                     NITALIKDLGANGLVGLITLGIIVWLFSNRSLA"
     gene            2423..2653
                     /locus_tag="VI319_11255"
     CDS             2423..2653
                     /locus_tag="VI319_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864465.1"
                     /translation="MIQRITKIGWLVVELAFMLVVLCVLLSLILGKDAGAFIASVAAN
                     TVDLLQKLPSGTLLGFFLIFVLYWTFKRREAR"
     gene            2863..3177
                     /locus_tag="VI319_11260"
     CDS             2863..3177
                     /locus_tag="VI319_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864466.1"
                     /translation="MKRLTLSFSAAALTFACVLVHAGETHWDALRVYKLADGRGVAVS
                     YPGEWWELSRTRVLGPGEPARFVDLEGRRIEIPADALARAADAKSVARPLEHRRVALR
                     AR"
     gene            complement(3226..3675)
                     /locus_tag="VI319_11265"
     CDS             complement(3226..3675)
                     /locus_tag="VI319_11265"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6864467.1"
                     /translation="MRILAALAALAIAGCAAVVPSPPPPGSAPAPAPEAAPPSSRTEN
                     PAVAGLLDAARADVASGRLVNAAASLERALRIEPRNPRLWAELARVRFKQGDYAQAES
                     TARRSNSWAGSDSALRAENWRLIAQSREARGDGEGARTALEQAERQR"
     gene            complement(3694..5994)
                     /gene="mrcB"
                     /locus_tag="VI319_11270"
     CDS             complement(3694..5994)
                     /gene="mrcB"
                     /locus_tag="VI319_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HEY6864468.1"
                     /translation="MPRLHLPWRLLRFALLSLLLVAAGFTFYLDFRVRSEFEGRRFAL
                     PARIYARPLELHAGLKVPLPDIVDELKDEGYREGRQESGDGWFYRSGDELEIAVRPFV
                     FWDGPQPARKLHVAFDGKAVASVKDADGKSLSLARLEPMAIGGIYPANNEDRVLVRLS
                     EVPKPLIDTLIATEDRNFYTHHGFDLRGIARAAWSTVRGAGTQGGSTLTQQLVKNFFL
                     TPERTLTRKGTELVMAVLLEAHYDKNEILETYLNEIYLGQDRDRAIHGVGLAALHYFG
                     KTVDHLTLAESALLVGMVKGPAVYDPYRHPQRALERRNLVLRETRERGAITMQDYAAA
                     RAAPLGVNARNAMGTSPHPAFLELVHRQLRREYDENDLRSEGLRIFTTLDPRVQGKAE
                     SVLARRLAQFDKEKRFGSPGLEGAVVVTDSQTGEVEALVGGRDVKFRGFNRALDAARP
                     VGSLLKPAIYLTALSEPSRYTLVTRLDDNPFVWKSNGAPDWEPANYDKKFHGEVPLRT
                     ALAQSYNVATARLGTDLGLDRVFGTIKRLGIEREMKPYASTLLGAIDLSPFEVAQMYQ
                     TIASGGFRTPLRAIREVTTQDGKPLKRYAIAVEPAFPPEPMYLITAAMQGVVREGTGQ
                     ALKNWIPPDTAVAGKTGTTDEQRDAWFAGFTGDRLAVVWVGYDDNRAAHLSGAASALP
                     VWGEMMSALGTEPLALPKPDGIEQVLIDPASGLRADSGCPGATELPFVQGSAPAERAP
                     CATSVGIVVEQVKQRAKSWLERLFGR"
     gene            6119..6433
                     /locus_tag="VI319_11275"
     CDS             6119..6433
                     /locus_tag="VI319_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY6864469.1"
                     /translation="MEQTAREPKRSDIDALAGPTLLEFGTSWCGHCRRAQPLIAEALA
                     AHPEVRHIKVEDASGKRLGRSFGVKFWPTLVFLKDGKEIEKLVRPASVEEIARALEAI
                     GG"
     gene            6437..6829
                     /locus_tag="VI319_11280"
     CDS             6437..6829
                     /locus_tag="VI319_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864470.1"
                     /translation="MTLPAKLYLGAAVVLVAGLCAAVAIYLTAGDDPDLSGAYEIIVV
                     DGKPVPIPATQSKAYVRQLQRLGGKASLLFAEMGEWFGSLWRGKQLAITVGWLTLAVS
                     TGLFLLGWWLSPSPAAGKADSSSLRSSE"
     gene            complement(6835..>7054)
                     /locus_tag="VI319_11285"
     CDS             complement(6835..>7054)
                     /locus_tag="VI319_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864471.1"
                     /translation="EIDLFRRLKRLARRERRSIVILHRHPILTSGRKVKLYRWRELWA
                     FGAKLILTGGRSLRRRESCFPWYDGRRD"
BASE COUNT         1019 a         2370 c         2578 g         1087 t
ORIGIN      
        1 ccagccgcca gccgccgagg agcacgccca ggagcggcag gaagttcgcc agcacgagcg
       61 cgagcgtcgg cacggccggc agcgggcgcg gcggctcggc ctccgccggt ttggccggta
      121 ccggtgcgct cgggatgacg agctcggggg gcgcggccgc cgtggacgcg ggcatcgcgc
      181 cgcgtatcgc ggcaacgatc cgctccgcgt cggcgggcgg catggggtcg ccgagcgtgc
      241 gcccgccctc gccgtaatcg aagcgaacgc gtgcgttgtc cccttcgggc tcggcgcgca
      301 cgttgcgcat gcgcgcgagg tcgtactcgg cgatcatccg cagcggaccg atccgcggcg
      361 cgtcgatcag cgctccgctg gagagccacg tcatcgggcg gtagaagcac agcagcaccg
      421 tgagcgcgcc cagcaccaga acggcgagcg accagccgag gagccagaac agccggaaca
      481 gcgtctgcat gagcgcgccg acgctgtgca tcgggtgcgc atcgagcccc ttcgcggcga
      541 gaacccctgc gccggcgaag accacgaaca tgacggcgac gatgacggcg gcgaggccgg
      601 cgccacggcg cggaaactcg agcggcgtcg cgcccgcagg atcggagagc gagaagcgcc
      661 accgctcggg gcgtgcgagc ggcttgcggg gcgcggcggc tgcggtggcc gggatcatga
      721 cgcgggcagt ctactccgca agcggccgcg cgagcgcggc gtatgtcacg cctagggtgc
      781 gagcgcccgg tacaggaacg ggaggctcac gtccatgcgg taatcgacgt ccgagtggtc
      841 gtcgtcgaat tcctcgtagg tgtggcggat tccggcgcgc gcgagccgcg cagacaggat
      901 gcgcatgccg taatggatgt ggtactggtc gcgccagccg caatcgaggt agatgccgcg
      961 cagcgaccgc aggctcgcgc ggtgtcgctc gaccaggttc accggatcgt gccggcgcca
     1021 gctcgcccag cgcccgggga ccggctcgcc cgactccagg ttgaacggca cgcgaaaccc
     1081 gttcggagcg cgcgggtccg ggtcgtacgt ggcggccatg cacaggttca tgatgcagtg
     1141 gccttccttc cccgagagct tctccttctt ccacacgtgc gcgaggaagc gccggatgcg
     1201 gccgtcgtcc tcgccggcgg cgagcccttt gcggcgcgat tcgcgcagcg cgtcgtagcg
     1261 gcccgcgcgc cggcgcggcg cgcggtgctt cgcgagctcg tcgagcgtgt tcggccagtc
     1321 gtggccatag acgaactcga agtaggcgtc gcccgaatgg ttcgcgacgg cgccccaggt
     1381 gcgcgcgtat ttcatgccgt gcacgatcgc gccgtagccg cccgacgatt tgccgaagca
     1441 gccgcggtgc tcgcgcgaag cgagggtgcg gaattcgcgg tcgacgaagg ggacgatctc
     1501 gcgcgtgaga tagtcggcat agtcgccgat cgcgctcgag ttcacgtact ggttgccgcc
     1561 gagcgcggtg aagcaatcgg gaaagaccag gattgcgggt cccatccgct ggtggcggat
     1621 cagccgcgcg acgcgctcgg gaacgttgtc gccgaacggc ttccagttcg tgtgcgcgag
     1681 ccccgagccc gtgaagccca cgagatcgta gagcacgggg aagcgccgcc gcgtaccgtc
     1741 gtcgtactgc ggcggcagcc agacgccgag ccgccgcacg tgcgggtcgc cgagcggatt
     1801 tccgcgcagg acgcgcgagt cgtgctcgag cacgacgagc gtccccgggg atccttgggg
     1861 tcgtttcagg gccatcgatc gctccttgta aaaactgccg cgaccggccg cggcgcatcc
     1921 cgcatggtac ggacgttgcc ggtattgagc catcggcagg ccgaccggcc gcgcaaaggc
     1981 aggctgcgcg gttgatcgac cgatatacaa ggacttgtag ccgcaagccg agccccccca
     2041 agcgatagtg gccgggcatt gaaatcgccc gtggcttgct ataccatggg cgcgccgcac
     2101 tatcggagtg cacgcggcgt tggatctcac ccaaaggggg gcttcatgaa gatgatcgaa
     2161 agcatcaaag gctggatcgg cgcgctcacg gacgtggggc tgatgttgct ggcccttgcg
     2221 atcgtggcgg ccctgctggt cgggggaagt ctgccgttct tcggatccgt cgtcggcaac
     2281 atcaccgcgc tgatcaagga tctcggcgcg aacggcctgg tggggctgat caccctcggc
     2341 atcatcgtct ggctgttctc caatcggtct ctcgcgtagc aacgcggcgc gggcgggctc
     2401 gtcccgcccc gccgcgaacg cgatgatcca gagaatcacc aagatcggct ggctcgtagt
     2461 cgagctcgcg ttcatgctcg tcgtcctgtg cgtgctgctc agcctcatcc tcggaaagga
     2521 cgccggggcc ttcatcgcct cggtggccgc caacacggtg gacctgctgc agaagcttcc
     2581 ttcggggacg ctgttgggct ttttcctgat attcgtgctg tactggacgt tcaagcgcag
     2641 ggaagcgcgc tgagcgcgca gcttacgggc cgctgacgca aggctgtcgc cgccgtacga
     2701 cagcggggtg gcgctgtgta atttttcgtc gggaaaaggc gcgaccgcgc acgcggtgcc
     2761 aactcctacg gcccggccac cccggcggtc ttcctatact ggcttcgagc ggtggcaccc
     2821 ccacgtcgat acccgccgcc gcacccgagg aaggagcccc ccatgaagcg cttgacgctg
     2881 tcgttctccg ccgccgccct gacgttcgcc tgcgtgctcg tccacgccgg cgaaacgcac
     2941 tgggacgcgc tgcgcgtcta caagctcgcc gacggccgcg gggtcgcggt ctcgtatccc
     3001 ggggagtggt gggagctgag ccggacccgc gtcctcgggc cgggcgagcc ggcgcggttc
     3061 gtcgacctag agggccgccg catcgagatc ccggccgacg cgctcgctcg cgcggccgat
     3121 gcgaagtcgg tcgcgcggcc gctcgagcac cgccgcgtgg cgttgcgcgc gcgctgaggc
     3181 gatccggtcg aaaaaagccg cgcgaccgcg cggctttttt tcgccttacc gctggcgctc
     3241 tgcctgctcg agcgctgttc tggcgccctc gccgtcgccg cgcgcctcgc gcgactgggc
     3301 gatgaggcgc cagttctcgg cgcgcagggc gctgtcggat cccgcccagg agttggatcg
     3361 ccgcgccgtg ctctcggcct gggcgtagtc gccctgcttg aagcgcacgc gcgcgagctc
     3421 ggcccacagg cgcggattgc gcggctcgat gcgcagcgcg cgctcgagcg acgccgcggc
     3481 gttgaccagc cgcccggagg cgacgtcggc gcgcgcggcg tccaggagcc ccgcgaccgc
     3541 ggggttctcc gtgcgcgacg agggcggcgc cgcttcgggc gcgggcgccg gggcggaccc
     3601 cggcggaggc ggagacggca cgacggcggc gcagcccgcg atcgcgagcg ccgcgagggc
     3661 ggcgaggatt ctcatgggac cgctattttg cgcctatctg ccgaacagcc gctcgagcca
     3721 gctcttcgcc ctctgcttca cctgctcgac cacgatcccg accgaggtcg cgcacggcgc
     3781 gcgctcggcc ggcgccgagc cctggacgaa gggcagctcg gtcgcgcccg ggcatccgga
     3841 atcggcgcgc agcccgcttg ccgggtcgat caggacctgc tcgatgccgt cgggcttggg
     3901 cagcgcgagc ggctcggtcc cgagcgccga catcatctcg ccccagacgg gcagcgcgct
     3961 cgcggcgccc gagaggtgcg ccgcgcggtt gtcgtcgtag ccgacccaga cgaccgccag
     4021 ccggtcgccg gtgaatcccg cgaaccaggc gtcgcgctgc tcgtcggtgg tgccggtctt
     4081 gccggcgacc gcggtgtcgg gcgggatcca gttcttgagc gcctgcccgg tgccttcgcg
     4141 cacgacgccc tgcatcgccg cggtgatcag gtacatgggc tcgggcggga acgcgggctc
     4201 gaccgcgatg gcgtagcgct tgagcggctt gccgtcctgg gtggtgactt cgcggatcgc
     4261 gcgcagcggc gtgcggaagc cgccgctcgc gatcgtctgg tacatctgcg cgacctcgaa
     4321 cggcgagagg tcgatcgcgc cgaggagcgt cgacgcgtag ggcttcatct cgcgctcgat
     4381 gccgaggcgc ttgatggtgc cgaagacgcg gtcgagcccg aggtcggtgc cgaggcgagc
     4441 ggtcgcgacg ttgtacgact gcgcgagcgc ggtgcgcagc ggcacctcgc cgtggaactt
     4501 cttgtcgtag ttcgccggct cccagtcggg cgcgccgttg ctcttccaca cgaacgggtt
     4561 gtcgtcgagg cgcgtgacga gcgtgtagcg cgacggctcg gagagcgcgg tgaggtagat
     4621 cgcgggcttg aggagcgacc cgaccgggcg cgcggcgtcg agcgcgcggt tgaagccgcg
     4681 gaacttcacg tcgcgcccgc cgaccagcgc ctcgacctcg cccgtctggc tgtcggtgac
     4741 gacgaccgcc ccctcgaggc cgggcgagcc gaagcgcttc tccttgtcga actgcgcgag
     4801 acgccgcgcg agcacggact cggcctttcc ctgcacgcgc ggatcgagcg tggtgaagat
     4861 gcgcaggcct tcggagcgca ggtcgttctc gtcgtactcg cgccgcagct gccggtgtac
     4921 gagctcgagg aacgccgggt ggggcgaggt ccccatggcg ttgcgcgcgt tgacgccgag
     4981 cggcgccgcg cgcgccgcgg cgtagtcctg catcgtgatc gcgccgcgct cgcgcgtctc
     5041 gcgcaggacc aggttgcggc gctcgagcgc gcgctgcggg tggcggtagg gatcgtagac
     5101 ggccggaccc ttcaccatgc cgacgagcag cgcgctctcc gcgagcgtga ggtggtcgac
     5161 cgtcttgccg aagtagtgca gcgccgcgag accgacgccg tggatcgcgc ggtcgcggtc
     5221 ctggcctagg tagatctcgt tgagataggt ctcgaggatc tcgttcttgt cgtagtgcgc
     5281 ctcgagcagc accgccatga cgagctcggt gcccttgcgc gtcagcgtgc gctcgggcgt
     5341 gaggaagaag ttcttcacga gctgctgcgt cagcgtgctg ccgccctgcg tgccggcgcc
     5401 gcgcaccgtg gaccacgccg cgcgcgcgat cccgcgcagg tcgaagccgt ggtgcgtgta
     5461 gaaatttcgg tcctcggttg cgatcagcgt gtcgatcagg ggcttcggca cctcggacag
     5521 ccgcaccagc acgcgatcct cgttgttcgc cgggtagatg ccgccgatcg ccatcggctc
     5581 gaggcgcgcg agcgagagcg acttgccgtc ggcgtccttc accgaagcga ccgctttgcc
     5641 gtcgaaggcg acgtgcagct tccgcgccgg ctgcggcccg tcccagaaca cgaacgggcg
     5701 caccgcgatc tcgagctcgt cgcccgagcg gtagaaccag ccgtcgcccg actcctgccg
     5761 tccctcgcgg tagccctcgt ccttcagctc gtcgacgatg tcgggcagcg gcaccttgag
     5821 acccgcgtgc agctcgagcg gacgcgcgta gatgcgcgcg ggcagcgcga agcggcggcc
     5881 ttcgaactct gagcggacgc ggaaatcgag atagaacgtg aagcccgcgg cgacgaggag
     5941 cagcgagaga agggcgaagc ggaggagacg ccaggggagg tgcaggcggg gcatggcggg
     6001 attttacttg ctggtgacca ttcgaagtga ttgcaaacag tccgccgaag gcaatgcgct
     6061 acgtcctaac gggcggtggg tgtcgatcca ggcgtcgttc ctgggcgaca atagaggcat
     6121 ggagcaaacc gcgagggaac cgaagcgctc ggatatcgac gcgctcgccg gaccgacgct
     6181 gctcgaattc ggcacgtcct ggtgcggcca ctgccgccgt gcgcagccgc tgatcgcgga
     6241 ggcgctcgcc gcgcatccgg aggtccgcca catcaaggtc gaggacgcga gcggcaagcg
     6301 cctcggacga tccttcggcg tgaagttctg gccgacgctc gtctttctca aggatgggaa
     6361 ggaaatcgag aagctggtgc ggccggcgtc ggtcgaggag atcgcgcggg cgctcgaggc
     6421 gatcggtggc tgacgcatga cgctcccggc gaagctctac ctcggcgcgg cggtcgtgct
     6481 cgtcgccggc ctttgcgccg cggtcgcgat ctatctgacc gccggcgacg accccgatct
     6541 ctcgggcgcg tacgagatca tcgtggtcga cgggaagccg gttccgattc ccgcgactca
     6601 gtcgaaggcc tacgtgcgcc agctgcagcg cctcggcggc aaggcgtcgc tgctgttcgc
     6661 ggagatgggc gaatggttcg gctcgctgtg gcgcgggaag cagctcgcga tcacggtcgg
     6721 gtggctgacg ctcgccgttt cgacgggatt gttcctgctg ggctggtggc tgtcgccgtc
     6781 gccggcggct ggcaaagcag attcttcgtc gctgcgctcc tcagaatgac aaggctagtc
     6841 gcgccggccg tcgtaccagg gaaagcagct ctcgcggcgg cgcaggctgc ggccgccggt
     6901 cagtatcagc ttcgcgccga aggcccacag ctcgcgccag cggtagagct tcaccttgcg
     6961 gccgctcgtg aggatgggat gccggtgcag gatcacgatc gaccggcgct cgcgccgcgc
     7021 gaggcgcttc aggcgccgga acaggtcgat ctcc
//
LOCUS       DATDMW010000262         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1201061, whole genome shotgun sequence.
ACCESSION   DATDMW010000262 DATDMW010000000
VERSION     DATDMW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201061"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VI319_11290"
     CDS             complement(<1..938)
                     /locus_tag="VI319_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864472.1"
                     /translation="MPEYLAPGVYVEEVSFRSKSIEGVGTSTTAFVGPTLKGPADSTT
                     PELLTSFSDFERIYGGIDDLAYGNDDRLNRNYVAHAVQAYFNNGGARLYVVRVYTPGT
                     GTGIATAKASPVLSTTANNVTTPNAWFEARFPGATFNGAGVIVQQKGAPIRGKSLENA
                     PDGTVVRVHPAKAANWVLDGNTWKVLGDDGTPGADVQNPGVGTANVWLIAQVPDGGTD
                     PKALDKPIALDDTDFDALKDKARVRIFPAAEVWLRADGAWTRVTRGGTAVSGNDAEVK
                     PADLAGVGRAYAITMDVEFRPVSGDGIRFEDLSPVAG"
     gene            complement(975..1739)
                     /locus_tag="VI319_11295"
     CDS             complement(975..1739)
                     /locus_tag="VI319_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864473.1"
                     /translation="MIAERVERRVLGAIEFVDAWTGARVLEPLSLDAPRLRLTRNASS
                     LYVIRDADGLSAYTEAFEKPPAVAASDYSLRVSDPQRRYVTRTVRVTLPRADTPLTDP
                     ASFLQPLSVQLYPSMARNAEPIWPVLRAFIFVTDAGRRVGVANALLRLTPAPDGLAPT
                     LALTDANGEGFLAVPGAPPIFSGTPPTHLFDATLLVALDAQFARRGDAASLPVPDPDR
                     ILDRIAANDASVQQLTLPVEQLSAGATQRVELEVTL"
     gene            complement(1736..>2078)
                     /locus_tag="VI319_11300"
     CDS             complement(1736..>2078)
                     /locus_tag="VI319_11300"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Pvc16 family protein"
                     /protein_id="HEY6864474.1"
                     /translation="VDLHFLFCLWLDTASREHTVLGWLLRELARYPVLDRGMTDSSGG
                     FASVDRMQFIPSELSLDDMSKLWQLLGLPYRASLTYVVRNVQIGPETDAASAPVVATR
                     FEHRDEVEASA"
BASE COUNT          301 a          705 c          764 g          308 t
ORIGIN      
        1 ccggcgacgg gcgagaggtc ctcgaagcgg atgccgtcac ccgagaccgg gcggaactcg
       61 acatccatcg tgatcgcgta ggcgcggccc acgccggcga gatcggcggg cttcacttcc
      121 gcgtcgttgc cgctcaccgc cgtgccgcca cgcgtgacgc gcgtccacgc gccgtccgcg
      181 cgcagccaca cttccgccgc cgggaagatg cgcacgcgcg ccttgtcctt gagcgcgtcg
      241 aagtccgtgt cgtcgagcgc gatgggcttg tcgagcgcct tcgggtccgt gcccccgtcg
      301 ggcacctgcg cgatgagcca gacgttcgcc gtgcccacgc cggggttctg cacgtcggcg
      361 ccgggcgtgc cgtcgtcgcc gaggaccttc cacgtgttgc cgtccaggac ccagttggcg
      421 gccttcgccg ggtgcacccg gaccacggtc ccatccggcg cgttctcgag gctcttgccg
      481 cggatcggcg cgcctttctg ctgcacgatc acgccggccc cgttgaacgt ggcgccgggg
      541 aagcgcgctt cgaaccacgc gttcggcgtc gtgacgttgt tggccgtggt ggagagcacc
      601 gggctcgcct tcgcggtcgc gatgcccgtg ccggtgccgg gcgtgtagac gcgcaccacg
      661 tagagccgcg cgccgccgtt gttgaaatac gcctgcacgg catgcgcgac gtagttgcgg
      721 ttcagccggt cgtcgttgcc gtaggcgagg tcgtcgatgc cgccgtagat gcgctcgaag
      781 tccgagaagc tcgtgaggag ctccggcgtc gtgctgtccg cggggccttt cagggtgggg
      841 ccgacgaagg ccgtcgtgct cgtgcccacg ccctcgatcg atttggaacg gaaactgact
      901 tcctcgacgt atacgccggg cgctaggtat tcaggcacga ctcacctccg gtcggaatga
      961 aaaggcgcgg acggtcacag ggtcacctcc agctccacgc gttgggttgc gccggcggac
     1021 agctgctcca ccggcagggt cagttgctgg accgatgcgt cgttcgcggc gatgcgatcg
     1081 aggatccggt ccgggtcggg cacgggcagg gaagcggcgt cgccacgacg cgcgaactgc
     1141 gcgtcgagcg cgacgaggag cgtcgcgtcg aacaggtgcg tcggcggcgt cccggaaaag
     1201 atcggcggcg cgccgggcac ggcgaggaat ccttccccgt tcgcgtccgt gagcgcgagc
     1261 gtgggcgcga ggccgtccgg cgcgggcgtg aggcgcagca gcgcgttcgc cacaccgacg
     1321 cgtcggcccg cgtccgtcac gaagatgaac gcgcgcagca ccggccagat gggctccgca
     1381 ttgcgcgcca tcgacgggta gagctggacg gacagcggct gcaggaagga agccggatcc
     1441 gtgagcggcg tgtccgcgcg cggcagcgtg acgcgcaccg tgcgcgtgac atagcggcgc
     1501 tgcggatcgg acacgcgcag cgagtagtcg ctcgcggcca cggccggcgg cttctcgaac
     1561 gcttccgtgt aggcggacag gccgtccgcg tcgcggatca cgtacaacga cgaagcgttg
     1621 cgcgtgaggc gcaggcgcgg cgcgtcgagt gacagcggct cgaggacgcg cgcccccgtc
     1681 caggcgtcca cgaactcgat cgcgcccagc acacggcgct cgacgcgctc ggcgatcatg
     1741 cgctcgcctc cacttcgtcg cgatgctcga agcgcgtggc gaccacgggc gccgaggcgg
     1801 catcggtctc ggggccgatc tgcacgttgc gcaccacgta ggtgaggctc gcgcggtacg
     1861 gcaggcccag gagctgccag agcttcgaca tgtcgtcgag cgagagctcg gaggggatga
     1921 actgcatgcg gtccacgctc gcgaagccgc cgctcgagtc ggtcatgccg cgatcgagca
     1981 ccgggtagcg cgcgagctcg cgcagcagcc atcccagcac cgtgtgctcg cggcttgccg
     2041 tgtcgagcca gaggcagaag aggaagtgca ggtcgacg
//
LOCUS       DATDMW010000263        22798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1325345, whole genome shotgun sequence.
ACCESSION   DATDMW010000263 DATDMW010000000
VERSION     DATDMW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22798
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325345"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VI319_11305"
     CDS             complement(<1..1154)
                     /locus_tag="VI319_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY6864475.1"
                     /translation="MANNNYKHWKLETDRDKLAWLSFDKQGASTNTLSQEALAELDAA
                     LDETRALAPRGLVIRSAKPSGFIAGADVEEFTRIESPQGALALVRRGWDTFNKLAALP
                     FPTVALIDGFCMGGGLELALACRYRIAVDQPGTRLALPEVMLGILPGWGGVKRLPRLV
                     GPLAALDMLMTGRSVDARRARRMGLVDAAVPPRIKENAARMTALEAPRPRRLSLAQAA
                     MNMPLARPLIAAMARRQLRAKARPEHYPAPYAILALWEHYDGDPLAPHPDDPASVASL
                     AASDTTKNLIRIFFLQERLKSLGKESDFRARHVHVVGAGVMGGDIAAWCVVCGMQASL
                     QDLDEGQTKKALGRAKSLFRRQLRKKQAIDAAVARLVADPQGTHIKHADVV"
     gene            complement(1167..2495)
                     /locus_tag="VI319_11310"
     CDS             complement(1167..2495)
                     /locus_tag="VI319_11310"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY6864476.1"
                     /translation="MSKPIYIVDGARTPFLKSRNRPGPFSASDLATAAGRALLLRQKF
                     APEDLDEVILGCAAPSVDEVNIGRVAALRMGCGEKVPGWTVMRNCASGMQSLDSAIAN
                     MLSGRSNLVLAGGVDALSRTPLIYSDAMVLWFSALAAAKGPGQRLARFARLPLKSMFA
                     PVIGIMRGLTDPYVGLLMGQTAENVAYRFGITRPEMDAYAARSHERVLRAQRSGWLDR
                     PHDGLAAEIAPLFAPDGALYQKDDGVREDSTAENLAKLKPFFDRKFGNVTAGNSSQIT
                     DGAAWLVLATEEAVRRHDLAPVGRIVDTEWAGLDPAHMGLGPVHAATPLLARQGLKPN
                     DLDAWEINEAFAAQVLGCLAAWQDERYCREQLGLPGAMGELDLDKLNVDGGAIALGHP
                     VGASGTRIVLHLLHALKRTGAGDGGRRPRRGVAAICIGGGLGGAMMIETV"
     gene            complement(2506..4833)
                     /locus_tag="VI319_11315"
     CDS             complement(2506..4833)
                     /locus_tag="VI319_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY6864477.1"
                     /translation="MSIAAWIVITLAVIAVAVLGVAPLRRALLSRPLLAWYRGQLPAM
                     SQTEQEAIDAGTVWWDGDLFSGRPDWSKLLAIPKPALSAEEQAFLDNETEQLCAMAND
                     WETTQIWQDLPPHVWQFIKDKGFLGLIIPKRYGGKEFSAYAHSQVIMKLATRCSAAAA
                     SVMVPNSLGPAELLLHYGTEEQKNHYLPRLAKGLEIPCFGLTNPSAGSDAAAIPDVGI
                     VCKGTWEGREIVGMRVTWEKRYITLGPISTLLGLAFRLRDPDGLLGNREDIGITCALV
                     PTATPGVNIGSRHNPLNAVFQNGPNWGKDVFMPLDWIIGGPRMAGQGWRMLMECLAAG
                     RSISLPSSATGYAKLAARATGAYARVRSQFKTPIGKFEGIEEPLARIGGNVYMMDSAR
                     RMTAGSVDLGEKPSVISAICKYHLTERGRAVVIDAMDIVGGKGICLGPNNWLGRSYQQ
                     LPIAITVEGANILTRSLIIFGQGAIRCHPYVLKEMAAAKARDVVAFDLALFGHVRHVI
                     ANNLRAFVGGITGAAIFTAPSGAAPETRAYYRRLSRMSAALAFVADVSMLVLGGSLKR
                     REKISARLGDVLSALYLCSATLKRYEDDGRPAADLPLLHWAMQDALARAAGALHGVIV
                     NYPSRVVGFGLRLAVFPLGQPYAPPSDRLGHEVARLLIAPSAARDRLTAGMYLPKSES
                     DPIGCLEAAFEAALQVEAIEAKLRAAAKAGDIDGASPEDLARAGVAARIITVEEEARL
                     KRFAKLRDEVIRVDHFPQDLGVSEARAPAPQRAAA"
     gene            complement(4855..5655)
                     /locus_tag="VI319_11320"
     CDS             complement(4855..5655)
                     /locus_tag="VI319_11320"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY6864478.1"
                     /translation="MARSRSGPRSNAGDGKSPEATRRRILEAAERLFLEHGFDGTSLR
                     MLTATARVNLAAVNYHFGGKEGLFREMIGARFDPLNRERIALLAAYERAAAGRPLECE
                     TLLNALFAPTLRLAREAKRDGARTDFLRLLGRAYVDPSPLLRRFLSERYAPAAARFKE
                     AFARTLPDLPKRELSWRLHFMMGALAYTLAGTDAWKLIAALSPERSPDRAAEESDAML
                     LRRLAPFLAAGLQAPLPAADREEPSIAATKTLQRRLRPPGRNGATARP"
     gene            5773..7580
                     /locus_tag="VI319_11325"
                     /pseudo
     CDS             5773..7580
                     /locus_tag="VI319_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            7825..8703
                     /gene="pbpG"
                     /locus_tag="VI319_11330"
     CDS             7825..8703
                     /gene="pbpG"
                     /locus_tag="VI319_11330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEY6864479.1"
                     /translation="MALAALSLPALGEEAMIRGLRSNAVLVLDQDSGEVLFGKNTHSV
                     LPIASITKLMTAMVTLDAGLDSSEVIPITKADVDKMKGSRSRLQVGAQLSRDDLLRLA
                     LMASENRAASALGRTYPGGTGAFVQAMNLKAYLLGMTGTHFADPTGLSSANVSTAEDL
                     AKLVNAAQGYTQIREYSTTSAHQVRIGRRTLAFHNTNRLIRNSSWEIGLSKTGYISEA
                     GRCLVMQAKLAGRKIIIVLLDSWGKYTRIADASRIRNWLDPSTAPRHLRKASVQMKVR
                     RGGSLNSTLPRAERPA"
     gene            8715..9704
                     /gene="egtD"
                     /locus_tag="VI319_11335"
     CDS             8715..9704
                     /gene="egtD"
                     /locus_tag="VI319_11335"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY6864480.1"
                     /translation="MDAERAPLPVEGVLRFYDFLPEANPFLADVLAGLARAQKSLPPK
                     YFYDDAGSRLFEQICVLPEYYLTRTELGVMQQNVAEMAQFIGPDAQLVELGSGAGIKT
                     RLLIERLLPPLYVPIDIDGRTLRSASAQLAELFPWLNIVGLCADYTLPLELPQFAGLP
                     IRRKAVFFPGSTVGNFTPEEALAFLRRVRRMAGAGGALLIGVDLKKDKATLEAAYDDA
                     AGVTAKFNLNLLERMNRELGADFKPERFRHKAFYDPIRGWVEMHLESRYAQVAHVGGK
                     RFDFAVGETIHTEISCKYSVAEFQELARKAGFSAQKVWTDPRQLFSVHGMLAV"
     gene            complement(9734..10699)
                     /gene="corA"
                     /locus_tag="VI319_11340"
     CDS             complement(9734..10699)
                     /gene="corA"
                     /locus_tag="VI319_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY6864481.1"
                     /translation="MLVNCVAYQDGRKLADITIEQISDYVSRPECFVWVGLKDPGPGE
                     LAVMQKEFSLHELAVEDSRHGHQRPKIEEYGDSLFAVLQTVEFEGDDLLVGEIDMFVG
                     RNYILSVRNGTKKGFAEVRSRCEREPDLLRHGSGFVFYALLDAIVDRYFPILEELETA
                     LESIEERIFAEGSPRANIEALYGSKQELMTLQHAVRPLLEAVGKLYGGRVPQVCAGMQ
                     DYYRDVYDHVVRINQSIDSLREMVTTAISVNLSMITLKENETTQRLAAYAALVAVPTM
                     IAGVYGMNFKYMPELDWVMGYPLSIGLMAAIDGYLFYRFRKAGWL"
     gene            10783..11994
                     /locus_tag="VI319_11345"
     CDS             10783..11994
                     /locus_tag="VI319_11345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY6864482.1"
                     /translation="MPAISRRTAALGTENAFVVLAEVNELARRGKDIISFCIGQPDFH
                     TPANVQDAAVRAIREGKHGYTPSAGIVELREAAASYMGAMRGLTISPDHVVVGAGAKP
                     FIAYAILSTTDHGVGDEVIYPNPGFPIYESQIAAHGAVPVPLHLREARGFAFDPAELE
                     AKITPKTKLLILNSPQNPTGGILSRRDLEAIAAILRRHPDVWVYADEIYSRLVYDGAF
                     ASIASLPGMYERTIISDGASKTWAMTGWRLGFASNPVLAPEFVRWVTNTDSCASQITQ
                     WAAVEAIRGPQDDAERMRASFLERRDLIVGLLNGVPGVSCLVPGGAFYAWPNVTEACR
                     MIGASDSEEFRRRLLNEAGVAVLADIHFGARVPGEGQHVRFSYAASNEAIRAGVTRMA
                     DFIRSNSRRVA"
     gene            12000..13073
                     /gene="mnmA"
                     /locus_tag="VI319_11350"
     CDS             12000..13073
                     /gene="mnmA"
                     /locus_tag="VI319_11350"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY6864483.1"
                     /translation="MAERIVVGMSGGVDSSVAALLLKRAGYDVVGLFMKNWEDDDDDE
                     YCSTRQDLIDAAAAADVIGVELEAVNFSAEYKDRVFAEFLREYSAGRTPNPDVLCNAE
                     IKFRAFLDHAMSLGAARIATGHYARLRAAGARVELLRAADRGKDQSYFLHRLTQEQLA
                     RVAFPVGELKKAEVRRIAREAGLPNHAKKDSTGICFIGERPFREFLNRYLPRVPGPMR
                     TPEGRTVGEHIGLAFYTIGQRKGVGIGGLRGSSGEAWYVAGKDLARNELIVVQGHDHP
                     LLLRDKLSAADLAWVSGEAPDPARAYGAKTRYRQADAACRVAGLGARALEVEFDAPQW
                     AVTPGQSVVLYRDEVCLGGGVIQ"
     gene            complement(13081..13689)
                     /locus_tag="VI319_11355"
     CDS             complement(13081..13689)
                     /locus_tag="VI319_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6864484.1"
                     /translation="MKLIGSLASPYTRKARVVLAEKKIDYEFEIDSPWDAATHVPERN
                     PLGKVPVLVLDDGTTLFDSRVIVEFLDNASPLARLLPQGNRERIEVRRWEALADGVLD
                     AAVLVRMERRRKPEQQSPEWLERQMGKVRAGLSAMNADLGDKAWCAGGSYSIADIAVG
                     VCLGWLDFRFPDLGWRDQHPNLARALARLSDRASFADTLPRE"
     gene            13811..14932
                     /locus_tag="VI319_11360"
     CDS             13811..14932
                     /locus_tag="VI319_11360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6864485.1"
                     /translation="MELRGEATATVLAPGAPGVAPARTRVLADAAGLAELAPRWDALA
                     AGGTPMAHSLWSRAAAEVFAGDALRVTTLARDGRACAIAPLVLRGSLLPRLEVLGSSE
                     TGEPFDPIHEDPTALRELLAALIETGMPLYAERVFADSPLMAELRAACRTRAIAITRP
                     AVGCPRIALDAAWRQPESHPGVARRSDLRRKQRYAERLGALAYEAMTPTPGQLDSLLE
                     EAFRVEASGWKGESGSALSRNARAGAFYRRYAAAACASGLLRLFFMRVDGQAIATVIA
                     VECAESLWTLKIGFDESFSRCSPGSLLTRYTIAWAAERGLRSYEFLGTAAAWTRDWTR
                     DERRCVSLRLYPLRPKALAAFALDALGWARERALRRRGP"
     gene            14997..16364
                     /gene="purB"
                     /locus_tag="VI319_11365"
     CDS             14997..16364
                     /gene="purB"
                     /locus_tag="VI319_11365"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY6864486.1"
                     /translation="MPLSPLSALSPLDGRYHQQLEPLRRHFSEAALIRYRVAIEIRWL
                     LALSDEGAVTEMEPFSRRTRDDLEGLVEAFSDDDAALVKTIEAETNHDVKSIEYWLKR
                     RLAGNPEAARVGEFVHFACTSEDINNLAYGLMLRDARDRVLLPALDRVIERVAALAKD
                     HAALAMLSRTHGQPASPTTLGKELANFAHRLRRARARIAAVSLQGKINGAVGNYNAHV
                     AAYPDLDWEAFARRFVEALGLEFNSHTTQIEPHDSVAEQLDAFARANAILLDLDRDLW
                     GYISLGYFRQKPKAGEVGSSTMPHKVNPIDFENSEGNIGVANALLRHLADKLPVSRWQ
                     RDLSDSTALRNVGVALGHTLLAYESCLRGLGKLEADPARLAADLDANWEVLGEAVQTV
                     MRRHGLPDPYEQLKALTRGKRIDGDSMRAFIRGLALPDSAKKRLLEMVPGSYTGLAEK
                     LARKA"
     gene            complement(16368..17237)
                     /locus_tag="VI319_11370"
     CDS             complement(16368..17237)
                     /locus_tag="VI319_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6864487.1"
                     /translation="MRNGYRLAITMLFVASAASALAQGDARRGEYVAKVGGCVGCHTE
                     DKEGAQPFAGGRALKTPFGTFYGPNITPDPNAGLGRWSEADFIRAMRFGERPDGANYF
                     PAFPYTSFTKATDGDLKDLWAYLRTLKPSAQPSRPHDVRFPYSWRWLVTIWKWFFFTP
                     GPYVAPANASAMLARGGYLADALGHCGECHTPRNFLGAMKKDRWLAGGKGPDDKTIPN
                     LTPTRLKKQSDAELKDFLVTGTTPDGDAPAEAMAEVIRNTTSQLAPADLDALIAYLRS
                     LPPLPEEKRDEKE"
     gene            complement(17303..17767)
                     /locus_tag="VI319_11375"
     CDS             complement(17303..17767)
                     /locus_tag="VI319_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6864488.1"
                     /translation="MKRTLVATGLALALGAGYSLSAFAQAKPEVLVKQRQAAMTLFGK
                     YWGPLGAMAAGKAPYNAETVKRNAGYLSVLDKMPWDGFSPETKGTSAKTAALPAIWNE
                     PDKFKQAQERLQSEVSKLVQVSQSGDEAAVKAQLGATGKACGGCHENFREKQ"
     gene            complement(17885..18967)
                     /gene="secF"
                     /locus_tag="VI319_11380"
     CDS             complement(17885..18967)
                     /gene="secF"
                     /locus_tag="VI319_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY6864489.1"
                     /translation="MEFFKIRHDIPFMRHALVFNIVSLITFLAAVWLLATRGLNFSVE
                     FTGGTVMEVKYRQAADPQKIRGELTGAGFADAQVQNFGTSQDVLIRLPLKKAEGGDTN
                     RATARQSELVYAALTSQPSPGEDAACAPMDALYAEMVKIPEPRDRPQDKLEQRETQRA
                     QLAGKCTEITRVEFVGPQVGSELAWNGALALLITAIGIVLYLAFRFEWKFGVAAIIAN
                     LHDVVIILGFFALFQWEFSLPVLAAVLAVLGYSVNESVVIADRIRENFRKMRKASVTE
                     VIDNAITRTISRTIITHGCTQLMVTSMLVFGGPALHYFALALTIGICFGIYSSVLVMA
                     PLVRWLGVRREDLVKPLKDKKQEAVV"
     gene            complement(18978..20837)
                     /gene="secD"
                     /locus_tag="VI319_11385"
     CDS             complement(18978..20837)
                     /gene="secD"
                     /locus_tag="VI319_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY6864490.1"
                     /translation="MNRYPVWVYATVAVALALGLLYTLPNFFGEAPAVQVSPARATVK
                     VDTAALGRVEEALKQAGIQPTGLFQDPNSIKVRLADYDTQIKARDVIDGALNTDRASP
                     SYTVALNLVSRSPAWLSAINARPMYLGLDLRGGVHFLLQVDMRAAIAKRAESLTGDVR
                     SLLRDRNVRHSGITREGNNIVVRFRDAQTRDRARSAIADQITELQLADAGAGDDLRLV
                     ATLRPEAAKRTQEFALRQNIQTLHNRINELGVAEPVIQQQGADRVVVQLPGVQDTARA
                     KEILGRTATLEIRMVDTENMNAAALAAAKAGQVPFGDEFYVERDGAPILVRKQVVLTG
                     DRLTDAQPGFDNQTHEPAVHLTLDATGARIFKDVTRENVGKRMAILLIEKGKGEVVTA
                     PVIRTEIGGGRVQISGRMTTAEANDVALLLRAGSLAAPMEIVEERTVGPSLGADNIKK
                     GFDSTVFGFSVLTLFIVVYYSIFGVFSALALCANLLLLIAVLSLLQATLTLPGIAAIA
                     LTLGMAIDANVLINERIREELRNGTSPQAAIAAGYERAFGTILDSNVTTLIAGLALLL
                     FGSGPVRGFAVVHCLGILTSIFSSVVVSRAIVNLVYGGRRKLERVPIGNTAWK"
     gene            complement(20839..21162)
                     /gene="yajC"
                     /locus_tag="VI319_11390"
     CDS             complement(20839..21162)
                     /gene="yajC"
                     /locus_tag="VI319_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY6864491.1"
                     /translation="MLISSAFAQSAAPTGDAGGWASMLPIVLMFVLLYFLMIRPQMKR
                     AKEHKSMTEALQKGDEVVTSGGVIGKITGVGEYLTLEIAPNTEIKVQKSAVQTLLPKG
                     TLKSL"
     gene            complement(21228..22358)
                     /gene="tgt"
                     /locus_tag="VI319_11395"
     CDS             complement(21228..22358)
                     /gene="tgt"
                     /locus_tag="VI319_11395"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY6864492.1"
                     /translation="MKFAVTHTDGAARRGTLQLAHGAVETPVFMPVGTYGTVKAMSPA
                     ELRDLGAQIVLGNTFHLWLRPGLEVIAAHGGLHRFMGWNGPILTDSGGYQVFSLGELR
                     KISEDGVQFASPVNGDRLFLTPEESMRIQRVLDSDVAMVFDECTPYPADEHAAGESMR
                     LSLRWAERSKRSHHGNLNALFGIVQGGMHERLRDESLAGLGAIGFDGYAIGGLSVGEP
                     KADMRRVLAHVAPRLPEGAPRYLMGVGTPEDIVAAVELGIDLFDCVLPTRNARNGWLY
                     TRRGDVKIRNARHRSDTGPLDPACGCHTCRNFTRAYLHHLQKVNEILGARLNTLHNLH
                     FYLGLMRELREAIAAGRLREFAAAFREERGAAPPDEDATAFE"
     gene            complement(22355..>22798)
                     /locus_tag="VI319_11400"
     CDS             complement(22355..>22798)
                     /locus_tag="VI319_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEY6864493.1"
                     /translation="TLHVGAGTFQPVRVRDLAQHRMHAEWYDIPEATVAAVAAAKRRG
                     GKVVAVGTTTVRCLEGSAAAAPNREGAIAAGAGETDLFIAPGFEFRVVDRLITNFHLP
                     KSTLIVLVSAFAGIERVRRAYAHAIERRYRFYSYGDAMLVDRIPG"
BASE COUNT         3427 a         7916 c         7899 g         3556 t
ORIGIN      
        1 acgacgtcgg catgtttgat gtgcgtgcct tgcggatcgg cgacgaggcg cgcgacggcg
       61 gcgtcgatcg cctgcttctt gcgaagctgg cggcggaaca ggctcttagc gcggccgagc
      121 gccttcttgg tctggccttc gtcgaggtct tgcaggctcg cctgcatgcc gcaaacgacg
      181 caccaggcgg cgatgtcccc gcccatgacg cccgcgccca cgacgtggac atggcgcgcg
      241 cggaagtcgc tctccttgcc gaggctcttc agccgctcct gcaggaagaa gatgcggatc
      301 aggttcttcg tcgtgtcgct cgcggcgagc gacgccacgg aagccgggtc gtcggggtgc
      361 ggcgcgagcg gatcgccgtc gtagtgctcc cacagcgcaa ggatggcgta cggcgcggga
      421 tagtgctcgg gccgcgcctt cgcccgcagc tgccggcggg ccatggcggc gatcagcggc
      481 ctcgcgagcg gcatgttcat cgccgcctgc gcaagggaca ggcggcgcgg ccgcggcgcc
      541 tcgagcgccg tcatgcgcgc cgcgttctcc ttgatccgcg gcgggacggc ggcgtcgacc
      601 aggcccatgc ggcgcgcgcg gcgcgcgtcg accgagcgcc cggtcatcag catgtcgagc
      661 gcggccaggg ggccgacgag gcgcgggagc cgcttcacgc cgccccagcc cggaaggatg
      721 ccgagcatca cctcgggcag cgccagcctc gtgccgggct ggtcgacggc gatgcggtag
      781 cggcacgcga gcgcgagctc gaggccgccg cccatgcaga agccgtcgat cagcgcgacg
      841 gtcgggaagg gcagcgccgc gagcttgttg aacgtgtccc agccgcggcg cacgagcgcg
      901 agcgcgccct gcggcgactc gatccgggtg aattcctcga cgtcggcgcc ggcgatgaag
      961 cccgagggct tcgccgaccg gatcacgagc ccgcgaggag ccagcgcccg ggtctcgtcg
     1021 agcgcggcgt cgagctccgc gagtgcttcc tgcgagagcg tgttcgtcga ggcgccctgc
     1081 ttgtcgaacg agagccaggc gagcttgtcg cggtcggtct cgagcttcca gtgcttgtaa
     1141 ttgttgttcg ccatgttcgt gcgaggtcag accgtctcga tcatcatcgc gcccccgagt
     1201 ccgccgccga tgcagatcgc ggcgacgccg cgccgcggcc ggcggccgcc gtcacctgcg
     1261 ccggtgcgct tcagcgcgtg cagcaggtgc agcacgatgc gggtgccgga tgcgccgacc
     1321 gggtgcccga gcgcgatcgc gccgccgtcg acgttgagct tgtcgaggtc gagctcgccc
     1381 attgcgcctg ggagcccgag ctgctcgcgg caatagcgct cgtcctgcca cgcggcgagg
     1441 cagcccagca cctgcgcggc gaacgcctcg ttgatctccc aggcgtcgag gtcgttcggc
     1501 ttcagtccct gacgcgcgag cagcggcgtc gcggcgtgga ccggccccag gcccatgtgc
     1561 gccgggtcga ggcccgccca ctcggtatcg acgatgcgtc cgaccggggc gagatcgtgg
     1621 cgccgcaccg cctcctcggt cgcgagcacg agccacgccg cgccgtcggt gatctgcgac
     1681 gagttgccgg cggtgacgtt gccgaacttg cggtcgaaga agggcttgag cttcgcgagg
     1741 ttctccgcgg tggagtcctc gcgcacgccg tcgtccttct ggtagagcgc gccgtcgggg
     1801 gcgaacagcg gtgcgatctc ggccgcgagg ccgtcgtgcg ggcggtcgag ccagcccgag
     1861 cgctgcgcgc gcagcacgcg ctcgtggctg cgtgccgcgt atgcgtccat ctccggccgc
     1921 gtgatcccga agcggtacgc gacgttctcg gcggtctgtc ccatcagcag gccgacgtaa
     1981 gggtcggtga gcccgcgcat gatgccgatc accggcgcaa acatcgattt gagcggcagc
     2041 cgcgcgaagc gcgcgagccg ctggccgggt cccttcgccg cggcgagcgc ggagaaccac
     2101 agcaccatcg cgtcggagta gatgagcggc gtgcgcgaca gcgcgtcgac gccgccggcg
     2161 agcacgagat tcgagcgccc cgagagcatg ttcgcgatcg ccgagtcgag agactgcatg
     2221 cccgatgcgc aattgcgcat cacggtccag ccgggcactt tctcgccgca acccatgcgc
     2281 agcgccgcca cgcggccgat gttgacctcg tcgacgctcg gcgcggcgca gccgagaatg
     2341 acctcgtcga gatcctcggg cgcgaacttc tgacgcagca gcagcgcgcg cccggcggcc
     2401 gtggccaggt cgctcgccga gaacggcccc gggcggttgc gcgacttgag gaacggcgtg
     2461 cgcgcgccgt cgacgatgta gatgggcttg ctcacggccg gtccctcagg ccgccgcgcg
     2521 ctgcggcgcc ggcgcgcgcg cctcggaaac gccgaggtcc tgcgggaaat gatcgacgcg
     2581 gatgacctcg tcgcgcagct tggcgaagcg cttgaggcgc gcctcctcct cgaccgtgat
     2641 gatgcgcgcg gcgacgccgg cgcgcgccag gtcctcgggc gaagcgccgt cgatgtcgcc
     2701 ggccttggcc gcggcgcgca gcttcgcctc gatcgcctcg acctggagcg cggcctcgaa
     2761 cgcggcttcc aggcacccga tcggatcgga ctcgctcttc ggcaggtaca tgcccgcggt
     2821 cagccggtcg cgcgcggcgg acggcgcgat gagcagccgc gcgacctcgt gcccgagtcg
     2881 gtccgacggc ggcgcgtacg gctgaccgag cggaaacacg gcgagccgca agccgaagcc
     2941 gacgacccgg ctcggatagt tcacgatgac gccgtgcagc gcgccggcgg cgcgcgcgag
     3001 cgcgtcctgc atcgcccagt gcagaagcgg caggtccgcg gcgggccggc catcgtcctc
     3061 gtagcgcttc agcgtcgcgg agcagagata gagcgccgag agcacgtcgc cgaggcgcgc
     3121 cgagatcttc tcgcgccgct tgagcgagcc gcccagcacg agcatggaga cgtcggcgac
     3181 gaacgcgagc gcggcggaca tgcgggagag ccggcgatag tacgcgcgcg tctccggcgc
     3241 cgcgcccgac ggcgcggtga agatcgccgc gccggtgatg ccgccgacga atgcacgcag
     3301 attgttcgcg atgacatgcc ggacgtggcc gaacaacgcc aggtcgaagg cgacgacgtc
     3361 gcgcgccttg gccgcggcca tctccttcag cacgtaggga tggcagcgga tcgcgccctg
     3421 gccgaagatg atgaggctgc gcgtcaggat gttggcgcct tcgacggtga tcgcgatcgg
     3481 cagctgctgg tacgagcgcc cgagccagtt gttcgggccg agacagatgc ccttgccgcc
     3541 gacgatgtcc atcgcatcga tcacgactgc gcgcccgcgc tcggtaaggt ggtatttgca
     3601 gatggccgag atcaccgagg gcttctcgcc gagatcgacc gaccccgcgg tcatgcggcg
     3661 cgccgagtcc atcatgtaaa cgttgccgcc tatgcgcgcg agcggctcct cgatcccttc
     3721 gaacttgccg atcggggtct tgaactggct gcgcacgcgc gcgtaggcgc cggtcgcgcg
     3781 cgcggcgagc ttggcatagc cggtcgccga cgagggcagc gagatcgagc gcccggccgc
     3841 gagacactcc atcagcatgc gccagccctg gccggccatc ctcggtccgc cgatgatcca
     3901 gtcgagcggc atgaacacgt ccttgcccca gttcgggccg ttctggaaca ccgcgttcag
     3961 cgggttgtga cggctgccga tgttgacgcc cggcgtcgcg gtcggcacca gcgcgcaggt
     4021 gatgccgatg tcctcccggt ttccgagcag gccgtcggga tcgcgcaggc gaaaagcgag
     4081 cccgagcagc gtcgagatcg gcccgagcgt gatgtagcgc ttctcccagg tgacgcgcat
     4141 cccgacgatc tcgcggcctt cccatgtgcc cttgcacacg atgccgacgt cggggatcgc
     4201 tgcggcgtcg gaacccgcgc tcgggttcgt cagcccgaag cacgggatct cgagaccctt
     4261 cgcgagccgc ggcaggtaat ggttcttctg ctcctcggtg ccgtagtgca gcagcaactc
     4321 cgcgggcccg agcgagttcg ggaccatcac cgacgcggcc gcggcgctgc agcgcgtggc
     4381 gagcttcatg atgacctgcg agtgcgcgta cgcggagaac tccttcccgc cgtagcgctt
     4441 cgggatgatg aggcccagga agcccttgtc cttgatgaac tgccagacgt gcggcggcag
     4501 gtcctgccag atctgcgtcg tctcccagtc gttcgccatc gcgcacagct gctcggtctc
     4561 gttgtcgagg aaggcctgct cctcggcgct gagcgcgggc ttcgggatgg cgagcagctt
     4621 gctccaatcg ggccggcccg agaacaggtc gccgtcccac cagacggtgc cggcgtcgat
     4681 cgcttcctgc tcggtctgcg acatcgcggg cagctgaccg cggtaccacg cgagcagcgg
     4741 ccggctgagc agcgctcggc gcagcggtgc gacgccgagc acggcgaccg cgatcacggc
     4801 cagggtgatg acgatccacg cggcgattga catgggcttc tccggttcgg tgtgctatgg
     4861 gcgcgccgta gcgccgttgc gcccgggcgg gcgcaagcgg cgctgcagcg tcttcgtggc
     4921 tgcgatcgaa ggctcttcgc ggtctgccgc cggcagcggc gcctgcaggc cggccgcgag
     4981 gaacggcgcc agccggcgca gcagcatggc gtcgctctcc tcggcggcac gatccggcga
     5041 gcgttccggg ctgagcgcgg cgatcagctt ccaggcgtcg gttcccgcca gagtataggc
     5101 gagagcgccc atcatgaagt gcaggcgcca cgagagctcc cgcttcggca ggtcggggag
     5161 ggtgcgcgca aaggcctcct tgaagcgcgc ggccgccggc gcgtagcgct cggacaggaa
     5221 ccgccgcagc aggggggacg gatcgacgta ggcccgcccg agcagccgca ggaagtcggt
     5281 ccgcgcgccg tcgcgcttcg cctcgcgcgc gagcctgagg gtcggcgcga acagcgcgtt
     5341 gagcagcgtc tcgcattcga gggggcggcc cgcggcggcc cgctcgtacg cggccagcag
     5401 cgcgatgcgc tcgcggttga gcgggtcgaa gcgcgcgccg atcatctcgc ggaacaaccc
     5461 ttccttgccg ccgaagtggt aattgactgc ggccagattt acgcgggcgg tggcggtcag
     5521 catgcgcagc gacgtgccgt cgaagccgtg ctcgaggaac agccgctcgg cggcttcaag
     5581 aatgcgccgg cgcgtcgcct cgggcgactt gccgtcgccg gcgttactgc gggggccgga
     5641 gcgggaacgg gccatgggat tcaaacgagt gtttaaatga tacgttggcg ggcgcgattg
     5701 tcaagttttc gcgggggctt tcgttgtggc ggggtcggcc ccatgtaaca atgccggaga
     5761 gcccgcgcca tcatggccca cgaacaccgc agccacaccc tgacgttccc agcacggaag
     5821 acggaaaccg gcggtcggcg ccgcagcgaa tggcgcgccc ttggcatgct ggcgccttat
     5881 ctctgggaat tccgggtgcg cgtggcggtc gcgctggtgt tcctgatcac cgcgaagctc
     5941 accaacgtcg ccgtgccgct gatcctgaag cgggtggtcg attcgctcga ctcctcggtc
     6001 gcgatcctgg tcctgccgct cgccctgctc gcggcgtacg ggctgctgcg tttctcgacc
     6061 acgctgttcg cggagctgcg cgacgtggtg ttcgtgcgcg tcacgcagcg cgcgatccgg
     6121 cgcgtcgcgc tgtccgtgtt ccgtcacatg cacagcctga gcctgcgctt ccacctcgag
     6181 cggcagacgg gcggcgtgtc gcgcgacatc gagcggggca cgcgcggcat ctcgacgctg
     6241 atgtcgtaca tgctgttctc gatcattccg gtcgtgctcg agttcagtct ggttgcggcg
     6301 atcctgctcg ccaagttcga ctggcggttc gcggccgtca ccttcagcgc ggtggtcgtc
     6361 tacattgcgt ttaccgtcac ggtcaccgag tggcgaatgg agatccgccg gcgcgcgaac
     6421 gagctcgact cgcgcgcgaa cacccgagcc atcgacagcc tgatcaacta cgagacggtc
     6481 aagtacttca acaacgagga gtacgaggcg cgccgctacg acgagaattt gcgcaagtac
     6541 gagtcggcgg cggtgcgcaa cgagatgtcg ctcggcatcc tcaacatcgg ccagagcctt
     6601 atcatcgcga tcgcggtgac cctgctcatg atcctcgccg cgcagggcgt ggtcgccaag
     6661 gcgctcacgc tcggcgacct cgtgctcgtc aacggcctgc tgatccagct ctacatcccg
     6721 ctcaacttcc tgggcatggt gtaccgcgag atcaagcagg cgctcgcgga catggaccgc
     6781 atgttccgtc tcctcaccga ggatcgcgag atccaggacc gaccgggcgc cgcggtgctc
     6841 ggcaagggcg gcgcctcggt gcgcttcgac agcgtcgact tctcgtacga gccgcggcgc
     6901 cagatcctct tcggcgtctc gttcgagatc cccgcggggg acaaggtcgc ggtggtcggc
     6961 gcgagcggct cgggcaagtc gacgctcgcg cggctgctgt tccgcttcta cgaggtgaac
     7021 accgggcgca tttcaatcaa cggcaccgac atccgcgact tcacccaggc gagcctgcgc
     7081 ggggcgatcg ggatcgtgcc gcaggacacg gtgctcttca acgacaccgt cctgtacaac
     7141 atccattacg ggcggccgga ggcgacgacg gaagaggtga tcgaggcggc gaaggccgcg
     7201 catatccacg agttcatcga gagcctcccc gaccgctacg agacgcgcgt cggcgagcgc
     7261 gggctgaagc tctcgcgcgc gcgatcctga agaatccgag catcctgatt ttcgacgagg
     7321 cgacctcggc gctggactcc gagaccgaga aaggtatcca ggccgagctg gaccgcatcg
     7381 cccgcggccg caccacgttg gtgatcgctc accggctatc gaccatcatg gatgccgacc
     7441 agatcctggt cctcgatgcg ggtcgaatcg tcgagcgcgg gcgtcatcgc gagctgctcg
     7501 cagcgggtgg tctgtacgcg caaatgtggg ccttgcagct cgaggaagat ctggaagccc
     7561 aggccgccgc tccgatataa cacgttgttt tcctggttct tatggatcag gctgcgcgct
     7621 ttccgaggac tgtcgccggg ccgcgacgcg tccgaccgga ctatggcaac gtcatctcgt
     7681 gctcgccttc gatgttaaat acgacctgta tcttcttatt ttccttgcgt attctcagat
     7741 gcgccgatac acttcgtgaa aacgctcgca acactgttta tcagggagaa tcctttgagc
     7801 aagatcaagg gcttactgtt cgcgctggcg ctggcagcgc tgtcgctgcc tgccctgggc
     7861 gaggaggcca tgatccgcgg cctgcgctcc aacgcagtgc tcgtgctcga ccaggattcc
     7921 ggcgaagtac tgtttggcaa gaacacccac tccgtgctcc cgatcgcgtc catcaccaag
     7981 ctgatgaccg ccatggtgac gctcgacgcg ggcctcgact cgagcgaagt catcccgatc
     8041 accaaggccg acgtcgacaa gatgaagggc tcgcgctcgc gcctgcaggt cggcgcccag
     8101 ctcagccgtg acgatctgct gcggctcgcc ctgatggcga gcgagaaccg ggccgcgtcg
     8161 gccctcggac gcacctatcc cggcggcacc ggcgccttcg tccaggcgat gaacctcaaa
     8221 gcttacctcc tcggcatgac cggcacgcac ttcgccgacc ccaccgggct ctcgagcgcg
     8281 aacgtctcga ccgccgagga cctggccaag ctcgtgaacg cggcgcaggg ctacacccag
     8341 atccgcgagt actcgacgac gtccgcgcac caggtgcgca tcgggcgccg cacgctcgcc
     8401 ttccacaaca ccaaccgcct gatccgcaac tcgagctggg agatcggcct gtcgaagacc
     8461 ggctacatca gcgaggccgg gcggtgcctg gtgatgcagg cgaagctcgc cggccgcaag
     8521 atcatcatcg tgctgctcga ctcgtggggc aagtacaccc gcatcgccga cgcctcgcgc
     8581 atccgcaact ggctcgatcc ctcgacggcg ccgcgccacc tcaggaaggc ctcggtgcag
     8641 atgaaggttc gccgcggcgg cagcctcaac tcgacgcttc ccagagcaga acgcccggca
     8701 tagaatcccg gcgcatggac gccgagcgcg caccccttcc cgtcgaaggg gtgctccgct
     8761 tctacgactt cctccccgaa gcgaacccgt tcctcgccga cgtgctggcg ggtctcgccc
     8821 gcgcgcagaa gagcctcccg ccgaagtact tctacgacga cgccggctcg cgcctgttcg
     8881 agcagatctg cgtgctcccc gagtactacc tgacgcggac cgagctcggc gtcatgcagc
     8941 agaacgtcgc ggaaatggcg caattcatcg ggcccgatgc tcagctcgtc gagctcggca
     9001 gcggcgcggg catcaagacg cggctactga tcgagcggct gttgccgcct ctctacgtgc
     9061 ccatcgacat cgacgggcgg acgctgcgct cggcttcggc gcagctggcc gagcttttcc
     9121 cctggctcaa tatcgtcggc ctgtgcgccg actacaccct gccgctcgag ctgccgcagt
     9181 tcgccgggct gccgatacgc cgcaaggccg tcttcttccc cggatcgacc gtcggcaatt
     9241 tcacgcccga ggaggcgctc gccttcctgc ggcgcgtgcg gcgcatggcg ggcgcgggcg
     9301 gcgcgctgct catcggcgtc gacctcaaga aggacaaggc gacgctcgag gcggcatacg
     9361 acgacgccgc gggcgtgacc gcgaagttca acctgaatct tctcgagcgc atgaaccgcg
     9421 agctgggcgc cgacttcaag cccgagcgct tccggcacaa ggcgttctac gacccgatcc
     9481 gcggctgggt cgagatgcac ctcgagagcc gctacgcgca ggtcgcgcat gtcggcggca
     9541 aacgcttcga cttcgccgtc ggcgagacca tccataccga gatctcgtgc aagtactcgg
     9601 tcgccgagtt ccaggagctc gcgagaaaag ccggcttctc ggcgcagaaa gtctggaccg
     9661 acccgcggca gctgttcagc gtgcatggga tgctggccgt ctaggccagc ccgacgccgc
     9721 gcgctccccc gcgctagagc cagccggcct tgcggaagcg gtagaaaagg tagccgtcga
     9781 tcgcagccat caggccgatc gacagcggat agcccatgac ccagtccaac tcgggcatgt
     9841 acttgaagtt catgccgtat acccccgcga tcatggtcgg gaccgcgacc agagcagcat
     9901 aggcggcgag ccgctgcgtg gtctcgttct ccttcagcgt gatcatggag aggttgaccg
     9961 agatcgcggt cgtcaccatt tcgcgcaagc tgtcgatcga ctggttgatg cgcaccacgt
    10021 ggtcgtagac gtcgcggtag taatcctgca tcccggcgca cacctgcggc acgcggccgc
    10081 cgtagagctt gccgaccgcc tcgagcagcg gccgcaccgc gtgctgcagc gtcatcagct
    10141 cctgcttcga gccatacagc gcctcgatgt tggcgcgcgg ggagccctcg gcgaagatgc
    10201 gctcctcgat gctctcgagc gccgtctcga gctcctccag gatggggaag tagcggtcga
    10261 cgatcgcgtc gagcagcgcg tagaacacga agcccgaacc gtggcgcagg aggtcgggct
    10321 cgcgctcgca gcgcgagcgc acctcggcga atcccttctt ggtgccgttg cgcaccgaga
    10381 ggatgtagtt gcggccgacg aacatgtcga tctcgccgac gagcaggtcg tcgccctcga
    10441 attccaccgt ctgaagcacg gcgaacagcg agtcgccgta ctcctcgatc ttgggccgct
    10501 ggtggccgtg gcgcgagtcc tcgaccgcga gctcgtgcag tgagaactcc ttctgcatca
    10561 ccgccagctc gcccgggccg gggtccttca ggccgaccca gacgaagcat tcgggccgcg
    10621 acacgtagtc gctgatctgc tcgatcgtga tgtcggcgag cttgcggccg tcctggtagg
    10681 cgacgcagtt gacgagcatg gattctcccg ggcgggatga tagcagccgg tgccgcggcc
    10741 ccataggcta gaattcgttt tttcagcccg ccgacacccc gcatgcccgc gatcagccgc
    10801 cgtaccgccg ccctggggac cgagaacgcg ttcgtcgtgc tcgccgaggt gaacgagctc
    10861 gcgcgccggg gtaaggacat catctcgttc tgcatcggcc agcccgactt ccacacgccg
    10921 gcgaacgtgc aggacgccgc cgtgcgcgcg atccgcgaag gcaagcacgg ctacaccccg
    10981 tccgccggca tcgtcgagct gcgcgaggcg gcggcgagct acatgggcgc gatgcgcgga
    11041 ctgacgatct cgcccgatca cgtggtggtc ggcgcgggcg ccaagccttt catcgcgtac
    11101 gcgatcctct cgacgaccga ccacggcgtc ggcgacgagg tgatctaccc gaatccgggc
    11161 ttcccgatct acgagtcgca gatcgctgcg cacggggccg tcccggtgcc gctgcacctg
    11221 cgcgaggcgc gcggcttcgc gttcgatccg gcggagctcg aggccaagat caccccgaag
    11281 acgaagctgc tgatcctgaa ctcgccgcag aacccgaccg gcgggatcct gtcgcgtcgc
    11341 gacctggagg ccatcgccgc gatcctgcgc cggcacccgg acgtctgggt gtacgccgac
    11401 gagatctact cgcggctcgt ctacgacggc gccttcgcat cgatcgcgtc gctgcccggg
    11461 atgtacgagc gaacgatcat ctcggacggt gcctcgaaga cctgggcgat gacgggctgg
    11521 cggctcgggt tcgcgtccaa ccccgtgctc gcgccggagt tcgtgcgctg ggtgacgaac
    11581 acggactcgt gcgcatccca gatcacgcaa tgggctgcgg tcgaggcgat ccggggtccg
    11641 caggacgacg ccgagcgcat gcgcgcgtcc ttcctcgagc ggcgggacct gatcgtgggt
    11701 ctcctgaacg gcgtaccggg cgtttcctgc ctggtcccgg gcggcgcctt ctacgcctgg
    11761 ccgaacgtca ccgaggcctg ccgcatgatc ggcgcgtccg actccgagga gttccgcagg
    11821 cggctgctga acgaggcggg ggtcgccgtg ctcgcggaca tccacttcgg agcgcgcgtc
    11881 ccgggcgagg gccagcacgt gcggttctcc tacgccgcct cgaacgaagc gatccgggcg
    11941 ggcgtgacgc gcatggcgga cttcatccgg agcaactcgc ggcgcgtcgc ctgatcggca
    12001 tggccgaacg catcgtcgtc ggcatgtccg gaggtgtcga ctcctccgtc gccgcgctgc
    12061 ttctgaagcg cgccggctac gacgtcgtcg gcctgttcat gaagaactgg gaggacgacg
    12121 acgacgacga gtactgctcg acgcgccagg acctgatcga cgcggcggcc gccgccgacg
    12181 tgatcggggt cgagctcgag gcggtcaact tctcggccga gtacaaggac cgcgtcttcg
    12241 ccgagttcct gcgcgagtac tcggcggggc gcacgcccaa ccccgacgtg ctgtgcaacg
    12301 ccgagatcaa gttccgcgcc ttcctcgacc acgcgatgtc gctcggcgcg gcgcgcatcg
    12361 cgaccgggca ctacgcgcgc ctgcgcgccg ccggggcgcg ggtcgagctg ctccgggcag
    12421 ccgaccgcgg gaaggaccag agctacttcc tgcaccggct gacccaggag cagctcgcgc
    12481 gcgtcgcctt ccctgtgggc gagctgaaga aggcggaggt gcgccgcatc gcgagggaag
    12541 cgggcctgcc gaaccacgcg aagaaggact cgaccggcat ctgcttcatc ggcgagcggc
    12601 cgttccggga gttcctgaac cgctacctgc cgcgcgtacc cgggccgatg cgcacgcccg
    12661 aaggcaggac cgtcggcgag cacatcggcc tcgcgttcta cacgatcggc cagcgcaagg
    12721 gagtcggcat cggcggcctg cgcggctcga gcggcgaggc ttggtacgtc gccgggaaag
    12781 acctcgcgcg gaacgagctg atcgtcgtcc agggccatga ccatccgctg ctgctgcgcg
    12841 acaagctgag cgccgccgac ctcgcctggg tgagcgggga ggcgcccgat ccggcgcgcg
    12901 cctacggcgc caagacgcgc taccgccagg ccgacgcggc ctgccgcgtc gcgggcctcg
    12961 gcgctcgggc gctcgaggtc gagttcgacg ccccgcaatg ggcggtcacg cccgggcagt
    13021 cggtcgttct ctaccgcgac gaggtctgcc tcggcggggg cgtgatccag tagcctgcga
    13081 tcactcgcgc ggcagcgtat ccgcgaacga cgcgcggtcg gagagcctgg ccagcgcgcg
    13141 cgccaggttc gggtgctggt cgcgccagcc caggtcggga aagcggaagt cgagccagcc
    13201 gaggcagacg ccgaccgcga tgtcggcgat ggagtaggag ccgcccgcgc accaggcctt
    13261 gtcgcccagg tccgcgttca tggccgagag ccccgcgcgc accttgccca tctggcgctc
    13321 gagccactcc gggctctgct gctcgggctt gcggcggcgc tccatcctga cgagcaccgc
    13381 ggcgtcgaga acgccgtcgg cgagcgcctc ccagcggcgc acctcgatgc gctcgcggtt
    13441 cccctgcggc agcaggcgcg cgagcggcga ggcgttgtcc aggaattcga cgatgacgcg
    13501 cgagtcgaac agcgtcgtcc cgtcgtccag cacgaggacc ggcaccttgc ccagcggatt
    13561 gcgctcggga acgtgcgtcg ccgcgtccca gggactgtcg atctcgaact cgtaatcgat
    13621 cttcttctcg gccaggacga cgcgggcctt gcgcgtatag gggctggcga gcgagccgat
    13681 gagcttcatg gggtcgaaac gggggtcaac ggatcttccg aggcgccgca atcatagcat
    13741 ggcgctccgg tcctaatggt ccgtccgcct gccgctgccg tgtcacggcg cgctgctacc
    13801 ttcggcatcc atggagctcc gcggcgaagc caccgccacc gtgctcgcgc ccggcgcgcc
    13861 gggcgtcgcg cccgcgcgga cgcgcgtgct cgcggacgcg gccggcctcg ccgagctcgc
    13921 cccgcgctgg gacgcgctcg ccgccggcgg cacgccgatg gcgcattccc tgtggtcgcg
    13981 cgccgccgcg gaggtgtttg ccggcgacgc gctgcgggtg acgacgctgg cgcgcgacgg
    14041 ccgcgcatgc gcgatcgccc cgctcgtcct gcgcggatcg ctgctgccgc ggctcgaggt
    14101 gctcgggtcg agcgagaccg gcgagccgtt cgatccgatc cacgaggacc ccaccgcgct
    14161 gcgcgaactg ctcgcggcgc tgatcgaaac cgggatgccg ctctacgccg agcgcgtctt
    14221 cgcggattcg ccgctcatgg cggagctgcg cgcggcgtgc cgcacacggg cgatcgcgat
    14281 cacgcgtccg gccgtcggct gcccgcggat cgcgctggat gcggcatggc ggcagcccga
    14341 gagccacccc ggggtcgcgc gccgatccga cctgcgccgc aagcagcgct acgccgagcg
    14401 gctcggggcg ctcgcatacg aggcgatgac gccgacgccc ggccagctcg attccctcct
    14461 cgaggaggcg ttccgtgtcg aggcttccgg ctggaagggc gagagcggat cggcgctgtc
    14521 gcgcaacgcc cgcgcggggg cgttctatcg ccggtacgcc gcggccgcgt gcgccagcgg
    14581 gctgctgcgg ctgttcttca tgcgcgtcga cggccaggcg atcgccacgg tgatcgcggt
    14641 cgagtgcgcc gagagcctgt ggaccctcaa gatcggcttc gacgagtcgt tctcgcgctg
    14701 ctcgccgggg tcgctcctca cccgctacac gatcgcctgg gccgccgagc gcgggctgcg
    14761 ctcgtacgag ttcctcggca ccgccgcggc gtggacgcgc gactggacgc gcgacgagcg
    14821 ccgctgcgtc tcgctgcgcc tgtatccgct gcggccgaag gccctcgcgg cctttgccct
    14881 ggacgcgctc ggctgggcgc gcgagcgcgc gctgcggcgc cgcggcccct gacgccgccg
    14941 cgggccccgg cgcccgcgtg gtaaaattca ggctgtccgt gtcacggatc cagccgatgc
    15001 cgctctcgcc gctcagcgcc ctgtcgccgc tcgacgggcg ctaccaccag cagctcgagc
    15061 cgctgcggcg tcacttcagc gaagcggcgc tcatccgcta ccgggtcgcg atcgaaatcc
    15121 gctggctgct cgcgctctcc gacgagggcg ccgtgaccga gatggagccc ttctcgcgca
    15181 ggacgcgcga cgacctcgag ggtctcgtcg aggcgttctc ggacgacgac gcggcgctgg
    15241 tgaagacgat cgaggccgag accaaccacg acgtcaagtc gatcgagtac tggctcaagc
    15301 gccgcctggc gggcaatccc gaggccgcgc gcgtcggcga gttcgtgcac ttcgcgtgca
    15361 cctcggagga catcaacaac ctcgcgtacg ggctgatgct cagggatgcc cgcgaccgcg
    15421 tgctgctgcc ggcgctggac cgcgtcatcg agcgcgtcgc ggcgctggcg aaggaccacg
    15481 ccgctctcgc catgctctcg cgcacgcacg gtcagcccgc gagcccgacc accctcggca
    15541 aggagctggc gaacttcgcg caccggctgc gccgcgcccg cgcgcgcatc gccgcggtct
    15601 ccctccaggg caagatcaac ggcgccgtgg gcaactacaa cgcccacgtc gccgcgtacc
    15661 cggacctcga ctgggaggcg ttcgcccgcc gcttcgtcga ggccctgggg ctggaattca
    15721 actcgcacac gacccagatc gagccgcacg actccgtcgc cgagcagctc gatgccttcg
    15781 cccgcgcgaa cgcgatcctg ctcgacctgg accgcgacct ctggggctac atctcgctcg
    15841 gctatttccg ccagaagccg aaggccggcg aggtcgggtc ctccaccatg ccgcacaagg
    15901 tgaacccgat cgacttcgag aactccgagg gcaacatcgg cgtggcgaac gcgctgctgc
    15961 ggcacctcgc cgacaagctg cccgtgtcgc gctggcagcg cgacctctcc gactcgactg
    16021 cgctgcgcaa cgtcggcgtg gcgctcggcc acacgctgct cgcgtacgag tcgtgcctgc
    16081 gggggctcgg caagctcgag gccgatcccg cgcgcctcgc cgccgacctc gacgccaact
    16141 gggaagtcct cggggaagcg gtgcagaccg tgatgcggcg ccacggcctt cccgatccct
    16201 acgagcagtt gaaggcgctc acgcgcggca agcgcatcga cggcgactcg atgcgcgcct
    16261 tcatccgcgg actcgcgctt cccgacagcg cgaagaagcg cctgctcgag atggtgccgg
    16321 gcagctacac cgggcttgcc gagaagctcg cccggaaagc ctaggcgcta ctctttctcg
    16381 tccctcttct cttccggcag cggcggcagc gagcgcaggt aggcgatcag cgcgtccagg
    16441 tccgcggggg cgagctggct cgtcgtgttg cggatcacct cggccatggc ctcggccggc
    16501 gcgtcgccgt cgggcgtcgt cccggtcacg aggaaatcct tgagctcggc gtcgctctgc
    16561 ttcttgaggc gcgtcggcgt caggttcggg atggtcttgt catcgggacc cttgccaccc
    16621 gcgagccagc ggtccttctt catcgccccg aggaaattgc gcggcgtgtg gcactcgccg
    16681 caatgcccga gcgcgtcggc caggtagccg ccgcgcgcca gcatcgccga agcattcgcc
    16741 ggggcgacgt acggaccggg agtgaagaag aaccacttcc agatcgtgac cagccagcgc
    16801 caggagtatg ggaagcgcac gtcgtgggga cggctcggct gcgcgctcgg cttcagcgtg
    16861 cgcaggtacg cccagaggtc cttgaggtcg ccgtcggtgg ccttcgtgaa cgaggtatag
    16921 gggaacgcgg ggaagtaatt cgcgccgtcg ggccgctcgc cgaagcgcat cgcgcggatg
    16981 aagtcggcct cgctccagcg gccgagcccc gcgttcgggt cgggcgtgat gttcgggccg
    17041 tagaacgttc cgaacggcgt cttgagcgcg cgcccgccgg cgaaaggctg ggcgccttcc
    17101 ttgtcctcgg tatggcagcc gacgcagccg cccaccttgg cgacgtattc gccgcgcctg
    17161 gcgtcgccct gggcaagcgc gctcgccgcg ctcgcgacga acagcatcgt gatcgccaga
    17221 cgatatccgt tgcgcatgct cgatagcccc cggaaaagaa agccccgccg aagcggggcc
    17281 cgtggcgaat cgtcgcgccg tgctattgct tctcgcggaa attctcgtgg cagccgccgc
    17341 aggccttgcc ggtcgcgccg agctgcgcct tcaccgcggc ttcatccccg ctctggctca
    17401 cctggaccag cttcgagacc tcgctctgca gccgctcctg tgcctgcttg aacttgtcgg
    17461 gctcgttcca gatcgcgggc agcgcggccg tcttggccga agtgcccttg gtctcggggc
    17521 tgaagccgtc ccagggcatc ttgtccagga cgctcagata gccggcgttg cgcttcaccg
    17581 tctcggcgtt gtacggcgcc ttgcccgccg ccatcgcgcc gagaggaccc caatacttgc
    17641 cgaacagcgt catcgccgcc tgacgctgct tgaccaggac ttcgggcttc gcctgcgcga
    17701 aggcgctcag gctatagccg gcgcccaggg ccagggcgag gcccgtcgct accagggttc
    17761 ttttcattcc agtgctcctc tcggatcagg ttgatatgcg ggtgtcatgc agcggcatcc
    17821 gccgcacgaa gctcattaac agcgcccggc ggcggtttat tccccaggcg gcctcggagt
    17881 atacctagac cacggcctcc tgcttcttgt ccttcagcgg tttcaccagg tcctcgcgcc
    17941 gtaccccgag ccagcgcacg agcggcgcca tgaccagcac cgacgagtag atgccgaagc
    18001 agatgccgat cgtgagggcg agcgcgaagt agtgcagcgc cggcccgccg aacaccagca
    18061 tcgacgtgac catgagctgc gtgcacccgt gcgtgatgat cgtgcgcgag atggtgcgcg
    18121 tgatcgcgtt gtcgatgacc tcggtgaccg aggctttgcg catcttgcgg aagttttcgc
    18181 ggatccggtc ggcgatgacg accgactcgt tcaccgagta gccgagcacg gcgagcaccg
    18241 ccgcgagcac cggcagcgag aattcccact ggaacagcgc gaagaagccg aggatgatca
    18301 cgacgtcgtg caggttggcg atgatcgccg cgacgccgaa cttccattcg aagcggaagg
    18361 cgaggtaaag gacgatgccg atggccgtga tcagcagggc gagggcgccg ttccaggcga
    18421 gctccgagcc cacctgcggc ccgacgaact cgacgcgggt gatctcggtg catttgcccg
    18481 cgagctgcgc gcgctgcgtc tcgcgctgct cgagcttgtc ctgcggtcgg tcgcgcggct
    18541 cggggatctt caccatctcg gcgtagagcg cgtccatcgg cgcgcacgcg gcgtcctcgc
    18601 ccggtgacgg ctgggacgtc agggcggcgt agaccagctc gctctggcgc gcggtcgcgc
    18661 ggttggtgtc gcccccctcg gccttcttca gcggcaggcg gatcagcacg tcctgcgagg
    18721 ttccgaagtt ctgcacctgc gcgtcggcaa agccggctcc ggtcagctcg ccccggatct
    18781 tctgcgggtc ggccgcctgg cggtacttca cctccatgac cgtcccgccc gtgaactcga
    18841 ccgagaagtt gagaccccgg gtcgcgagca gccagaccgc ggccaggaag gtgatcaacg
    18901 agacgatgtt gaacaccagc gcatgccgca tgaacgggat gtcgtgccgg atcttgaaga
    18961 attccatcag ccggccctca tttccacgcg gtgttcccga tcggcacgcg ctcgagcttg
    19021 cggcgtccgc cgtagaccag gttgacgatc gcgcggctca ccaccaccga cgagaagatc
    19081 gacgtcagga tgccgaggca gtgcaccacg gcgaagccgc gcaccggccc ggagccgaac
    19141 agcaggagcg cgagtccggc gatcagcgtg gtgacgttcg agtccaggat cgtgccgaag
    19201 gcgcgctcgt agcccgcggc gatcgcggcc tgcgggctgg tgccgttgcg cagctcctcg
    19261 cggatgcgct cgttgatgag gacgttcgcg tcgatcgcca tgccgagcgt gagcgcgatt
    19321 gcggcgatcc cgggcagcgt cagcgtcgcc tgcagcagcg acagcaccgc gatcagcagc
    19381 agcaggttcg cgcacagcgc gagcgccgag aacacgccga agatggagta gtagacgacg
    19441 atgaacagcg tcagcaccga gaagccgaag acggtggagt cgaagccctt cttgatgttg
    19501 tcggcgccga gcgaggggcc gacggtgcgc tcctcgacga tctccatcgg cgccgcgagc
    19561 gatccggcgc gcagcagcag cgcgacgtcg ttcgcctccg cggtggtcat gcggccgctg
    19621 atctgcacgc ggccgccgcc gatctcggtg cggatcacgg gcgcggtgac gacttcgccc
    19681 ttgcccttct cgatcagcag gatcgccatg cgcttgccga cgttctcgcg cgtcacgtcc
    19741 ttgaagatgc gcgcgccggt cgcgtccagt gtgaggtgca cggcgggctc gtgggtctgg
    19801 ttgtcgaacc cgggctgggc gtcggtgaga cggtcgcccg tgagcaccac ctgcttgcgc
    19861 acgaggatcg gagcgccgtc gcgctcgacg tagaattcgt cgccgaaggg cacctggccg
    19921 gccttcgccg cggcgagcgc ggccgcgttc atgttctccg tgtccaccat gcggatctcg
    19981 agcgtggcgg tgcggccgag gatctccttc gcgcgcgcgg tgtcctggac gccggggagc
    20041 tggacgacga cgcggtccgc gccctgctgc tggatcacgg gctcggccac gcccagctcg
    20101 ttgatccggt tgtgcagggt ctggatgttc tggcgcagcg cgaactcctg ggtgcgcttc
    20161 gccgcctcgg gcctgagcgt cgcgaccagc cgcaggtcgt cgcccgcccc ggcgtcggcg
    20221 agctgcagct ccgtgatctg gtcggcgatg gccgagcgcg cccggtctcg cgtctgcgcg
    20281 tcgcggaagc ggaccacgat gttgttgccc tcgcgcgtaa ttcccgagtg ccgcacgtta
    20341 cggtcgcgca gcagcgagcg cacgtctccg gtcaggctct cggcgcgctt cgcgatcgcc
    20401 gcgcgcatgt cgacctgcag caggaagtgc acgccgccgc gcaggtcgag cccgaggtac
    20461 atgggccggg cgttgatcgc ggagagccac gccggcgagc gcgacacgag gttgagcgcg
    20521 acggtgtacg agggactggc gcggtcggtg ttgagcgcgc cgtcgatgac gtcgcgcgcc
    20581 ttgatctgcg tgtcgtagtc ggcgaggcgc accttgatgc tgttcggatc ctggaacagg
    20641 ccggtcggct ggatcccggc ctgcttcagc gcctcctcga cgcgcccgag cgcggcggtg
    20701 tcgaccttca ccgtggcgcg cgccggcgag acctgtaccg cgggcgcctc gccgaagaag
    20761 ttcggcagcg tatagagcag cccgagcgcg agcgcgacgg cgacggtcgc gtagacccag
    20821 accgggtagc ggttcatgct agagggactt gagcgtgccc ttcggcagca gcgtctgcac
    20881 cgccgacttc tggaccttga tctcggtatt gggcgcgatc tcgagcgtca ggtactcgcc
    20941 gacgccggtg atcttgccga tgacgccgcc cgaggtgacg acctcgtcgc ccttttgcag
    21001 cgcctcggtc atcgacttgt gctccttggc gcgcttcatc tgcggccgga tcatcaggaa
    21061 gtacagcagc acgaacatga gcacgatcgg cagcatcgaa gcccagcccc ccgcgtcgcc
    21121 ggtgggggcc gccgactgcg caaaggcgga agagatcagc acgaaggtct ccagtctcgg
    21181 gtaccaggga gcgaccgaaa attatacttg agccgaggag cgcgcgctca ctcgaacgcg
    21241 gtggcgtcct cgtccggggg cgccgcgccg cgctcttcgc ggaacgccgc cgcgaactcg
    21301 cgcagccgtc ccgcggcgat cgcctcgcgc agctcgcgca tcagcccgag atagaagtgc
    21361 aggttgtgga gggtgttgag acgcgcgccc aggatctcgt tcaccttctg caggtgatgc
    21421 aggtaggcgc gcgtgaagtt ccggcaggta tggcagccgc acgccgggtc gagcggaccc
    21481 gtgtccgagc ggtgccgggc attgcggatc ttgacgtcgc cgcggcgggt gtagagccag
    21541 ccgttgcgcg cattgcgcgt cggcagcacg cagtcgaaca ggtcgatgcc gagctcgacc
    21601 gccgcgacga tgtcctcggg cgtgccgacg cccatcaggt agcgcggcgc gccctcgggc
    21661 aggcgcggcg ccacgtgcgc gagcacgcgg cgcatgtccg ccttgggctc gccgaccgag
    21721 agccccccga tcgcgtagcc gtcgaagccg atcgcgccca ggcccgcgag cgactcgtcg
    21781 cgcaggcgct cgtgcatgcc gccctggacg atgccgaaga gcgcgttgag gttgccgtga
    21841 tgcgatcgct tcgagcgctc cgcccagcgc agcgacagcc gcatcgactc gcccgccgcg
    21901 tgctcgtcgg cggggtaggg cgtgcactcg tcgaagacca tcgcgacgtc ggagtcgagc
    21961 acgcgctgga tgcgcatcga ctcctcgggc gtgaggaaca ggcggtcgcc gttcacgggc
    22021 gaggcgaact gcacgccgtc ctcactgatc ttgcgcagct cgccgaggct gaacacctgg
    22081 tagccgcccg agtcggtgag gatcggcccg ttccagccca tgaagcggtg cagcccgccg
    22141 tgcgccgcga tcacctcgag cccggggcgc agccagagat ggaaggtgtt gccgaggacg
    22201 atctgtgcgc cgagatcgcg caattccgcc gggctcatcg ccttcaccgt gccgtaggtg
    22261 ccgaccggca tgaacaccgg cgtctcgacc gcgccgtggg cgagctgcag ggtcccgcgg
    22321 cgcgcggcgc cgtcggtgtg cgtgaccgcg aatttcatcc ggggattcta tcaaccagca
    22381 tcgcgtcgcc gtacgagtag aagcgatacc gccgctcgat cgcgtgcgcg tacgcgcgcc
    22441 gcacgcgctc gatgccggcg aaggccgaga ccagcacgat cagcgtcgac ttcggaagat
    22501 ggaaattggt gatcagccgg tcgacgaccc tgaactcgaa tcccggcgcg atgaagagat
    22561 cggtctcgcc cgccccggcg gcgatcgcgc cctcgcggtt cggcgcggcg gcggccgagc
    22621 cctcgaggca gcgcaccgtg gtcgtgccga ccgcgaccac cttgccgccg cgccgcttcg
    22681 ccgccgcgac ggcggcgacc gtcgcctccg ggatgtcgta ccactcggcg tgcatccggt
    22741 gctgcgcgag atcgcgcacg cgcaccggct ggaaggtgcc ggcgccgacg tgcagggt
//
LOCUS       DATDMW010000264         5464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2198692, whole genome shotgun sequence.
ACCESSION   DATDMW010000264 DATDMW010000000
VERSION     DATDMW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198692"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VI319_11405"
     CDS             complement(<1..1224)
                     /locus_tag="VI319_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6864494.1"
                     /translation="MTEILERAATREPGLAELRALVDLAPDGIFIADIEGRYTFVNEA
                     GCRLLGRVAGELIGKSIVDLIPDDEAGRLLASKERMLLGESHSGEWHLRRADGAWVPV
                     EVNAKILPDGRWIGFVRDVSDRRADEAERDAALKAAESDRRWLNTVLETMPFGVLLFQ
                     PGGQLYFNRRTEELLGIKLSPTGGSAQYAGRILYPDGRPVPPEELLTSRVLREGKTII
                     AAEFRVLRPDGTQIPVLSSAAPIRDADGGVIGGVGAFQDMSERMRMEQAIRANERLLR
                     GIFAILPVGVWVADKTGRIVSNNPAGERIWRGARYVPSAEFGQYKGWWADTGKPIAAE
                     EWALARAITKGETSDAELIRIQCFDGSFKTIINTAAPLRDEHGEIAGAIVVNEDISAL
                     QHTQEQLRAAVRDREE"
     gene            1383..2354
                     /locus_tag="VI319_11410"
     CDS             1383..2354
                     /locus_tag="VI319_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864495.1"
                     /translation="MQPRLSAAIAAAVLALAAADAGAQSAYPSKPVRFIVPSSAGGGT
                     DIIARAISQKLSEALGQQFVVDNRPGAGQMIGIELAARSPADGHTILMTASTLAINPI
                     MYKKVTYDPVRDFAPITQAASLPNVLVVHPSLPVKSLAELIALAKREPGRIAYASAGI
                     GTSPHMSVALLEHLAGIEMVHVPYKGTGPGVIDVLAGQVKVMTPNVLTALPHIKSGKL
                     RALAVTSGKRSDALPDVPTIAEAGVPGYESVQWYGVLAPAGTPREIVQRLYTEIAKSL
                     RAGDVRERLAADGAEPVGSTPEQFAAFIRAEIDKWAKLAKAAGIKPE"
     gene            complement(2391..2891)
                     /locus_tag="VI319_11415"
     CDS             complement(2391..2891)
                     /locus_tag="VI319_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864496.1"
                     /translation="MKRKIAFVALPVLLVLGACVSMPSGPSILVLPGTGKNFDQFRFD
                     ETDCRSYASSLLGGKDANSAAAEAGLKSAAVGTAIGAVAGAAIGGRSGAAVGAGGGAL
                     VGGAAGTGAASTSQYGLQRRYDNAYIQCMYAKGHRVPVAGSFTSAPPANQPPPPPPPP
                     PPPPPR"
     gene            complement(2902..3246)
                     /locus_tag="VI319_11420"
     CDS             complement(2902..3246)
                     /locus_tag="VI319_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864497.1"
                     /translation="MTKLIKSTLIAALVAAGALASGSALAWHRGGAHVGVFIGGPVWW
                     GYPYPYYAYPPTVVVREAPPTQYIEQSTPDASAQQQGYWYYCAESKTYYPYVKDCPGG
                     WQKVVPQTQPAR"
     gene            complement(3273..3608)
                     /locus_tag="VI319_11425"
     CDS             complement(3273..3608)
                     /locus_tag="VI319_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864498.1"
                     /translation="MNKTFTMLLAGVAIAAASGAAMASDVRFSVSVGVPAPYVYSPPV
                     TYAPPVVLAPAPVYDPPRVVYAPAPAYPYYVAPRVVYGPRVVVAPWWAYRHGYGRYWD
                     HERYEHRRW"
     gene            3768..4745
                     /locus_tag="VI319_11430"
     CDS             3768..4745
                     /locus_tag="VI319_11430"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864499.1"
                     /translation="MKRPATRIAALAACLIVSMASAPATAQDWPTKPVKLVVPFPPGG
                     SVDPLARLLGAKLSESLGQQFIIENRTGASGAIGTAYAAKQPADGYTFVVTFDTHAII
                     PFLQKLPYDTEKDFDPVMLVGTAPLAITTQPSKRYHDVADIIAAAKAKPDAVTFGSIG
                     NGSVGHLSMTVFMQRAGIRLVHVPYKGGGPMMQDALGGAIELGVASVAALAANVKGGK
                     LRAIATTGDKRAHTMPEVPTLAERGYAGFSAADWWMIVAPAGTPKPVIAKLHAELVKA
                     IRLPEINKNLTETLGMDVVAASPEDTQKFLLAEMARWGKVVRDNDIRAD"
     gene            4957..>5464
                     /locus_tag="VI319_11435"
     CDS             4957..>5464
                     /locus_tag="VI319_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY6864500.1"
                     /translation="MLTRTIIAAALAGAFAVPLAVHAADYGTRSSSTPSASTRGGAQA
                     DGSNTSQSDIANTPGKPGSSSPSSGEHAGVSASEFSRLDKNHDGYLSKDEVKLHPVLR
                     SRFASLDKDHDGKLSQSEVSSGTTASTESRTRLSRNDTDTTGSSTSNVNPSRSTRGAD
                     NHAVTGSAG"
BASE COUNT          863 a         1936 c         1875 g          790 t
ORIGIN      
        1 ctcctcccgg tcgcgcaccg cggcgcgcag ctgctcctgc gtgtgctgca gcgccgagat
       61 gtcttcgttg acgacgatcg cgcccgcgat ctcgccgtgc tcgtcgcgca gcggggcggc
      121 ggtgttgatg atggtcttga acgagccgtc gaagcactgg atgcggatca gctccgcgtc
      181 ggaggtctcg cccttggtga tcgcgcgcgc cagcgcccat tcctcggcgg cgatgggctt
      241 gccggtatcg gcccaccagc ccttgtactg cccgaactcg gcgctcggca cgtagcgggc
      301 gccgcgccag atgcgctcgc ccgcgggatt gttgctgacg atgcggccgg tcttgtcggc
      361 gacccatacg cccaccggca ggatggcgaa gatgccgcgc agcaggcgct cgttcgcccg
      421 gatcgcctgc tccatgcgca tgcgctcgct catgtcctgg aaggcgccga cgccgccgat
      481 aacgccgccg tcggcgtcgc ggatcggcgc ggcgctgctg agcaccggga tctgcgttcc
      541 gtcggggcgc aggacccgga actcggccgc gatgatggtt ttgccctcgc gcagcacccg
      601 gcttgtgagc agctcctcgg gcgggaccgg gcgcccgtcg gggtagagga tgcgacccgc
      661 gtactgcgcg ctgcccccgg tcggcgagag cttgatgccg agcagctcct cggtgcggcg
      721 gttgaagtac agctgcccgc ccggctggaa cagcagcacc ccgaacggca tcgtctcgag
      781 cacggtgttc agccagcgcc ggtccgactc ggcggccttg agcgcggcgt cgcgctcggc
      841 ctcgtccgcg cggcggtcgg acacgtcgcg cacgaagccg atccagcggc cgtccggaag
      901 aatcttcgcg ttcacctcga ccgggaccca ggcgccgtcc gcgcgccgca ggtgccattc
      961 tccggaatga ctttccccga gcagcatcct ttccttcgac gcgagcaggc gcccggcctc
     1021 gtcatcgggg atgaggtcca cgatgctctt gccgatcaac tcgccggcca cgcggccgag
     1081 caaccggcag cccgcctcgt tgacgaaggt gtagcggcct tcgatgtcgg cgatgaagat
     1141 gccgtcgggg gcgagatcga cgagcgcccg gagctccgcg aggcccggct cccgcgtcgc
     1201 cgctctctcg aggatttcgg tcatcagcga tgatcgcgcg ccgggccggg ccgaattttg
     1261 atccccgtca aaggccgggg tgcggaagtc cgaccggacc atccccggtg ccggtcgcga
     1321 ccgtgatgca taatgccggg cgttccgcgc gaccatcgac agcgacaagg aggaggagat
     1381 ccatgcagcc gcgcctttcc gccgcgatcg cggccgccgt cctcgcgctc gccgccgcgg
     1441 acgccggcgc gcagagcgcg tacccttcca agcccgtgcg cttcatcgtg ccctcgtccg
     1501 cgggcggcgg caccgacatc atcgcgcgcg cgatctcgca gaagctctcc gaggcgctcg
     1561 ggcagcagtt cgtggtcgac aaccggccgg gcgccgggca gatgatcggc atcgagctcg
     1621 ccgccaggtc gcccgccgac ggccatacca tcctgatgac ggcgagcacg ctcgcgatca
     1681 atccgatcat gtacaagaag gtcacctacg acccggtgcg cgacttcgcg ccgatcacgc
     1741 aggccgcgtc gcttccgaac gtgctggtcg tgcacccgtc gctgccggtc aagtcgctcg
     1801 ccgagctgat cgcgctcgcg aagcgcgagc ccggcaggat cgcgtacgcg tcggccggca
     1861 tcggcacgtc gccgcacatg tcggtcgcgc tgctcgagca cctggccggg atcgagatgg
     1921 tgcacgtccc gtacaaggga acgggccccg gcgtgatcga cgtgctcgcg gggcaggtga
     1981 aggtcatgac cccgaacgtg ctcacggcgc tgccgcacat caagtcggga aagctgcgcg
     2041 cgctcgcggt caccagcggc aagcgcagcg acgcgctgcc cgacgtgccg acgatcgccg
     2101 aggccggcgt tccgggctac gagtcggtgc agtggtacgg cgtgctcgcc cccgcgggta
     2161 cgccgcgcga gatcgtgcag cggctgtaca cggagatcgc gaaatcgctg cgcgcgggcg
     2221 acgtgcgcga gcggctcgcc gccgacggcg ccgagcccgt cggcagcacg cccgagcagt
     2281 tcgccgcgtt catccgcgcc gagatcgaca aatgggcgaa gctggcgaag gccgccggga
     2341 taaagcccga gtgataaacg aacaggccgc gcggggcggc ctgtcctgga ttacctggga
     2401 ggaggcggtg gcggcggcgg gggcggcgga ggcggctggt tcgccggcgg cgccgaggtg
     2461 aagctgcccg cgaccgggac gcggtggccc ttggcgtaca tgcactggat gtacgcgttg
     2521 tcgtagcggc gctgcagccc gtactgcgag gtcgaagccg cgccggtgcc cgccgcgccg
     2581 ccgacgagcg cgccgccacc cgcgccgacc gcggcgcccg agcggccgcc gatcgccgcg
     2641 ccggcgaccg cgccgatcgc agtgccgacg gccgcgctct tcaggccggc ctcggccgcg
     2701 gccgagttcg cgtccttgcc gccgagcagc gagctcgcgt aactgcggca atcggtctcg
     2761 tcgaagcgga actgatcgaa attcttgccg gtgcccggga gcaccaggat gctcgggccg
     2821 gagggcatgg acacgcacgc gccgagcacg agcagcaccg gcagcgcgac gaatgcgatc
     2881 ttgcgtttca tgtgcgttct ctcagcgcgc gggctgcgtc tgcggcacga ccttctgcca
     2941 gccgccgggg caatccttga cgtacgggta gtacgtcttc gactccgcgc agtagtacca
     3001 gtagccctgc tgctgcgccg atgcgtcggg ggtgctctgc tcgatgtact gggtcggcgg
     3061 cgcctcgcgt accaccaccg tcggcggata cgcgtagtac gggtacggat atccccacca
     3121 caccggcccg ccgatgaaca cgccgacgtg cgcgccgccg cgatgccagg cgagcgccga
     3181 gccgctcgcc agcgcgccgg ctgccacgag ggccgcgatc agcgtcgact tgatgagctt
     3241 ggtcatgccg gatctccttt ggacgaggcc ggctaccagc gccgatgctc gtaccgctcg
     3301 tggtcccagt aacgtccgta gccgtgccga tacgcccacc acggagcgac taccacgcgc
     3361 gggccgtaga cgacgcgcgg cgccacgtag tacggatagg ccggcgcagg agcgtagacg
     3421 actctgggcg gatcatacac tggggcagga gccaggacca cgggcggcgc ataagtcaca
     3481 ggcggagaat agacgtaagg cgcgggcacg ccgaccgaga cgctgaatcg aacgtcgctc
     3541 gccatcgcgg caccgctcgc cgcggcgatc gcgacgccgg ccagaagcat agtgaaagtc
     3601 ttgttcacga ggggtctcct tcgctcgggc ggctcgccca ttccctccaa cgcttatgat
     3661 ccgccgcggc ggacccgcga tctgcgaaaa aaggtaacac tgtgttgtcg cgggcgatcc
     3721 gacgttacac tttgttaccc tttcggcttt cgaggaggac gagaaccatg aagagacccg
     3781 ccacccggat cgcggcgctc gccgcgtgcc tgatcgtctc gatggcgtcg gcgcccgcga
     3841 cggcgcagga ctggccgacc aagcccgtga agctcgtcgt cccgttcccg ccgggcgggt
     3901 cggtcgatcc gctcgcgcgc ctgctcggcg cgaagctctc cgagtcgctc ggccagcaat
     3961 tcatcatcga gaaccgcacc ggcgcgtcgg gcgcgatcgg caccgcgtac gcggcgaagc
     4021 agccggccga cggctacacc ttcgtggtca ctttcgatac gcacgcgatc atcccgttcc
     4081 tgcagaagct gccttacgac acggagaagg acttcgaccc cgtgatgctg gtcggcacgg
     4141 cgcctctcgc gatcacgacg cagccgtcga agcgctacca cgacgtcgcc gacatcatcg
     4201 ctgcggcgaa ggcgaagccc gatgcggtca cgttcggctc gatcggcaac ggcagcgtcg
     4261 gccacctgtc gatgacggtc ttcatgcagc gcgcgggcat caggctcgtg cacgtgccct
     4321 acaagggcgg cggaccgatg atgcaggacg cgctcggcgg cgcgatcgag ctcggcgtcg
     4381 cgtcggtcgc cgcgctcgcc gccaacgtga agggcggcaa gctgcgcgcg atcgcgacca
     4441 ccggcgacaa gcgcgcccac acgatgcccg aggtgccgac tctcgccgag cgcggatacg
     4501 ccggcttctc cgccgcggac tggtggatga tcgtggcgcc ggcgggcacg ccgaaacccg
     4561 tgatcgcgaa gcttcacgcc gagctggtca aggcgatccg acttcccgag atcaacaaga
     4621 acctcacgga gacgctcggc atggacgtgg ttgccgcgag ccccgaggac acgcagaaat
     4681 tcctgctcgc ggagatggcg cgctggggta aggtcgtccg cgacaacgac atccgcgcgg
     4741 actgagctcg agcgtatagc ccttccgggc tgtcttcacc gggcgccggt aattccggcg
     4801 cccgcctctg caattttttc ccgccgaccc gcggacagcg gccgcgcagg ctctgcaaat
     4861 ggtgctcgcg gagcatggca cgccgcttgc gagtatccgg gccatcccag gcccacgcgg
     4921 gatacttttc aattgtctgc cagaggagac atccgaatgt tgacgagaac catcattgcc
     4981 gccgcgctcg ccggcgcgtt cgcagtgccg cttgcggttc acgcggccga ttacggaacc
     5041 cggtccagct ccaccccgag cgcgagcacg cgcggcggcg cccaggccga cggctccaac
     5101 acctcgcaaa gcgacatcgc caatacgccc ggcaagccgg gctcgagctc gccgagctcg
     5161 ggcgagcacg ccggggtgag cgcgagcgag ttctcgcgcc tcgacaagaa ccacgacggc
     5221 tatctgtcga aggacgaggt caagctgcac ccggtcctgc gcagccgctt cgcgtcgctc
     5281 gacaaggacc acgacggcaa gctgtcgcag tccgaagtga gcagcggcac gacggccagc
     5341 accgagagcc ggacgcgctt gagccgcaac gacaccgaca cgaccggcag ctcgacgtcg
     5401 aacgtgaacc cgtcgcgctc cacgcgcggc gcggacaacc acgcggtcac cggcagcgcc
     5461 ggga
//
LOCUS       DATDMW010000265         2866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1948704, whole genome shotgun sequence.
ACCESSION   DATDMW010000265 DATDMW010000000
VERSION     DATDMW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948704"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VI319_11440"
     CDS             complement(<1..1058)
                     /locus_tag="VI319_11440"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY6864501.1"
                     /translation="MADYRIERDTFGEIRVPAERLWGAQTQRSLENFRISDERMPTEL
                     IRALAKVKRACAAVNRDLGALDAKKADAIVAAADEVLAGKQDAEFPLVVWQTGSGTQT
                     NMNMNEVLANRASELLGGERGEKRLVHPNDDVNKGQSSNDVFPTAMHVAAVEALHGRV
                     IPSVRALRETLAAKSAEFRDIVKIGRTHLQDATPLTLGQEFSGWVSQLDHGVAHVQAS
                     LTHLCELAVGGTAVGTGLNAHPEFGARVAKELQRLTPGIPFVGARNKFEALAANDALV
                     HAHGALKTLAASLTKIANDVRWLASGPRSGIGEITIPENEPGSSIMPGKVNPTQSEAM
                     TMLCAQVMGNDVAINIGGA"
     gene            1105..2127
                     /locus_tag="VI319_11445"
     CDS             1105..2127
                     /locus_tag="VI319_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6864502.1"
                     /translation="MDRKIDIYNHVMPHKYLEMMKVHSKDPGIVKRMTSLRMLWDIEA
                     RVKMLDEKFPDVQQVLTLSLPSPELVGGPELSPELARIANDGMADMVAKWPDKFPAFV
                     ASLPMNNVPAALEEMDRAIGKLGARGVQIATSVNGKPLDDPEYFPVFERVTNKHDLPI
                     WMHPARPATRADYVNEPKSKYEIWQVLGWPFETSVAMSRMVFSGFFEKLPAMRLITHH
                     CGAMIPFFAGRAETLWAQLGSRSADENYEELLKSMKKKPIEYFKMFYADTVLGGSASA
                     LRCGLDFFGVDKVVFASDCPFDPEGGPMFIREGIRSIEDLKLSDADKRKLYFGNALKL
                     LRMPAR"
     gene            complement(2134..>2866)
                     /locus_tag="VI319_11450"
     CDS             complement(2134..>2866)
                     /locus_tag="VI319_11450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6864503.1"
                     /translation="SVLIAGCGTGRHAAITARQQPHARILAVDLSAASLAFAMRRFRE
                     LGITNVRFARADLLRLGGLQERFDLIECAGVLHHLDDPIAGWRVLAGLLRSKGFMNVA
                     LYSESGRSAIVAARRLIEARGLAPDPAGMRAARDMIMALPQGAPERRVMDSVDFWSLS
                     GCRDLLFHAREHRFTLARIAAALRELDLEFLGFDVDSRVKRAYAERFPGDRAAADLEN
                     WAAFEAEHPDTFAAMYQFWVRRKRA"
BASE COUNT          447 a          992 c          972 g          455 t
ORIGIN      
        1 gcgccgccga tgttgatcgc gacgtcgttg cccatcacct gggcgcacag catcgtcatc
       61 gcctcggact gcgtcggatt cacttttccg ggcatgatcg agctgcccgg ctcgttctcg
      121 gggatcgtga tctcgccgat gccgctcctc gggccggagg cgagccagcg cacgtcgttc
      181 gcgatcttgg tgagcgaggc ggccagcgtc ttcagcgcgc cgtgcgcgtg cacgagcgcg
      241 tcgttcgccg cgagcgcctc gaacttgttg cgggcgccga cgaacgggat cccgggcgtg
      301 agtcgctgga gctctttcgc gacgcgcgcg ccgaactccg ggtgcgcgtt caggccggtc
      361 ccgaccgcgg tgccgccgac cgcgagctcg cacaggtgcg tgagcgacgc ctgaacgtgc
      421 gcgacgccgt gatcgagctg cgagacccat cccgagaatt cctgcccgag cgtcagcggc
      481 gtggcgtcct gcaggtgcgt gcgcccgatc ttgacgatgt ccctgaactc ggcggatttc
      541 gccgcgagcg tctcgcgcag cgcgcgcacc gacggaatca cgcggccgtg cagcgcctcg
      601 accgcggcga cgtgcatcgc cgtggggaag acgtcgttcg acgactggcc cttgttcacg
      661 tcgtcgttgg gatggaccag gcgcttctcg ccgcgctcgc cgccgagcag ctcgctcgcg
      721 cggttggcga gcacctcgtt catgttcatg ttggtctgcg tccccgagcc ggtctgccag
      781 acgaccagcg ggaactcggc gtcttgcttg cccgcgagga cctcgtcggc ggcggcgacg
      841 atggcgtcgg ccttcttcgc gtcgagcgcg ccgaggtcgc ggttgacggc ggcgcaggcg
      901 cgcttcacct tggcgagcgc gcggatgagc tcggtcggca tgcgctcgtc cgagatgcgg
      961 aagttctcga gcgagcgctg cgtctgcgcg ccccagaggc gctccgcggg gacacggatc
     1021 tccccgaagg tgtcgcgctc gatccgatag tcggccatgg cgatagttta tgctacgcca
     1081 aattcccaac ccgcgaggcc gcccgtggac cgcaagatcg atatctacaa ccacgtcatg
     1141 ccgcacaagt acctggagat gatgaaggtt cactccaagg atcccggcat cgtcaagcgc
     1201 atgaccagcc tgcgcatgct gtgggacatc gaggcgcggg tgaagatgct cgacgagaag
     1261 ttccccgacg tgcagcaggt gctgactctc tcgctgccct cgcccgagct ggtcggcggg
     1321 cccgagctct cgcccgagct cgcgcgcatc gccaacgacg gcatggccga catggtggcg
     1381 aagtggccgg acaagttccc cgcgttcgtc gcctcgctcc cgatgaacaa cgtcccggcc
     1441 gcgctcgagg agatggaccg cgcgatcggc aagctcggcg cgcgaggtgt gcagatcgcg
     1501 accagcgtca acggcaagcc gctcgacgat cccgaatact tcccggtgtt cgagcgcgtc
     1561 acgaacaagc acgatctgcc gatctggatg cacccggcgc ggccggcgac gcgcgccgac
     1621 tacgtgaacg agccgaagtc gaagtacgag atctggcagg tgctcggctg gccgttcgag
     1681 acgagcgtcg cgatgtcgcg catggtgttc tcgggcttct tcgagaagct gccggcgatg
     1741 cgcctcatca cgcaccactg cggcgcgatg atccccttct tcgccggccg cgccgagacc
     1801 ctttgggccc agctcggcag ccgcagcgcc gacgagaact acgaggagct tctcaaatcg
     1861 atgaagaaga agccgatcga gtacttcaag atgttctacg ccgacacggt cctcggcggc
     1921 tcggcctcgg cgctgcgctg cgggctcgac ttcttcggcg tcgacaaggt ggtgttcgcg
     1981 agcgactgcc cgttcgatcc cgagggcggg ccgatgttca tccgcgaggg catccgctcg
     2041 atcgaggacc tgaagctctc cgacgccgac aagcgcaagc tctacttcgg caacgcgctc
     2101 aagctgctgc gcatgcccgc gcgctaggtc gtctcaggcc cgcttgcggc gcacccagaa
     2161 ctggtacatc gcggcgaacg tgtcggggtg ctcggcctcg aaggccgccc agttctcgag
     2221 gtcggccgcg gcgcggtcgc cggggaaacg ctcggcgtag gcgcgcttca cgcgcgagtc
     2281 cacgtcgaac ccgagaaact ccaggtcgag ctctcgcagc gcggcggcga ttcgtgcgag
     2341 cgtgaagcgg tgctcccgcg cgtggaagag caggtcgcgg cagcccgaga gcgaccagaa
     2401 atcgaccgag tccatcacgc ggcgctccgg cgcgccttgc ggcaaagcca tgatcatgtc
     2461 gcgcgcggcg cgcatccccg ccggatccgg cgcaagaccg cgcgcctcga tgaggcgccg
     2521 ggccgcgacg atcgccgagc gtcccgactc gctgtagagc gcgacgttca tgaacccctt
     2581 cgatcgcagc agcccggcga ggacgcgcca tcccgcgatc ggatcgtcca agtggtgcag
     2641 cacgcccgcg cactcgatga gatcgaaccg ctcctgcagc cccccgagcc gcaggagatc
     2701 cgcgcgcgcg aagcgcacgt tcgtgatccc gagctcgcgg aagcgccgca tcgcgaacgc
     2761 gaggctcgcc gcgctcagat ccactgcgag gatacgggcg tgcggctgct ggcgcgccgt
     2821 gatcgccgcg tgccggccgg tgccgcatcc cgcgattagc acggac
//
LOCUS       DATDMW010000266        17873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2323938, whole genome shotgun sequence.
ACCESSION   DATDMW010000266 DATDMW010000000
VERSION     DATDMW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17873
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323938"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VI319_11455"
     CDS             complement(<1..423)
                     /locus_tag="VI319_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY6864504.1"
                     /translation="MRHGLIAWSRDEVPPSALDARVAKLQAAMNKQALDCVLVYTSFA
                     RPAAVAWLTHFVPYWNDALLAVFASGAPVLLTAFSKRMSAWIEETCHVGEVRAAPDLG
                     KAAAALLAEKRIALVGVVELAALPWRIAEPMQERDRADA"
     gene            complement(420..1535)
                     /locus_tag="VI319_11460"
     CDS             complement(420..1535)
                     /locus_tag="VI319_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY6864505.1"
                     /translation="MSPSSRALREERRARIDAALAEAGLDALVVAGDPWRSDYLRYAV
                     DATPLEGRAIAILERGGAARLFVDSPAEGARFARELPDVAVSASRSAITEAERAIGAL
                     IVKRVALAPLAAVPCRLAKGPFRKTIAEGTALLDRLMLVKSPREIDAVRKAAALADEG
                     YRVFREAARAGRAEYEPVAEVEAFFRSRGCPENFQILASGGREVRGMHPPGERRLREG
                     DLVTTELTPCVDGYYAQICRTLVIGRASGEQRRAFAVYDEALRAGLAALAPGATAGDV
                     ARAENGIFRREGLGDYVTSEYTRVRGHGLGLYVDSRPMLLEDVALEFRPGMTLIVHPN
                     TYHPEVGYFVHGDSAVVTESGCEVLTRTARALFEVPA"
     gene            complement(1538..2500)
                     /locus_tag="VI319_11465"
     CDS             complement(1538..2500)
                     /locus_tag="VI319_11465"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864506.1"
                     /translation="MLRRLLLGALIFASCAASAQQWPSKPIHVVVPNTAGSVGEVLLR
                     AIAPQVESKLGQRLLVDNKPGAAGNIGTAEVARAAPDGYTLLLAPTANYAVNQHLFPN
                     LGFDPLAALEPIAMLAEAPLLAVINAEVPARSLKELEQTVRANPGKLNYGAPGTGSPS
                     HLTGALFSQLTGNAMVYVPYKGIAPMVQALLANDIQVTFPTITTVLGALKTGRVRVLA
                     VMGRERLPELPDVPTTVEAGFPDLVFGNWWVLSAPRGTDARTVERLAREVDAALADPA
                     VRKRIRDAGHLPTGLSRADTAAFLRSESARYKKIVETGRIKPEL"
     gene            complement(2500..3456)
                     /locus_tag="VI319_11470"
     CDS             complement(2500..3456)
                     /locus_tag="VI319_11470"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864507.1"
                     /translation="MKRVVALFFALVSAALAPPALSDTFPSRPVRLVVPFPPGGPLDI
                     SGRLIGQHLSERWREPVVIENKPGSTIGPESVVKSAPDGYTLMIISSTPLVTLPQMQK
                     VPYDVLHDLTGVTQTTLLTYALVVNPASGIKSIDDLIGRAKRSPGKLNYASAGNGSGQ
                     HLYMELLKSAAGIDLTHIPFKGAAPALQAVMAGEIPMMLDVTVSTVPLVKSGKARAVL
                     VTGSEPLEQLPGAVPFDRLFPGLGIGSWHGIFAPGATPKPVLEQIASDIREVLKLPAV
                     GERFRQLGVEPSGLSGDEFNAVVRRDYERWGEIIGRNHLRAD"
     gene            3558..4712
                     /locus_tag="VI319_11475"
     CDS             3558..4712
                     /locus_tag="VI319_11475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6864508.1"
                     /translation="MESSVWRDRVLRWAGAFVATAVALVLRDTLEPLLGGSVPFSTLY
                     AAVAVVVLIGGYGPALAAMIGGYVAAEYFFVQPVHTAFVSAPPDPLRLALYTLSCLII
                     IGFGGGMRSARRRLEREVARRKRLEEKLREADKRKDVFLATVAHELKHPLAPIRNVLE
                     VLELKDLPDRELRWARGIIDRQVKNLARLVDDLLDVSRIVRGNMELRREPLAVSRIVA
                     DAVEVCRPPAQAQRHELAVALPRQPLWVEGDLTRLTQVVVNLLDNAVKYTPPGGHVAV
                     SAERDGDAALIVVRDDGIGIPESMLERVFEPFARSDEARARAAGGMGIGLSVVRDLVE
                     LHGGGIEARSAGRDRGSEFRVRLPLCATPPAREPGSAPGRTFPRAEAPAP"
     gene            complement(4639..5595)
                     /locus_tag="VI319_11480"
     CDS             complement(4639..5595)
                     /locus_tag="VI319_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY6864509.1"
                     /translation="MKICYFDDFRLGIVKGDAVVDVSKAVKSVPHTGPHDLISALIAD
                     FPKYRAKLEKAAAAGRGIPLKKVRLRSPLPRPVNIDCMAVNYMEDGTRSEPAPINAFH
                     KSPGAVIGDGDTMVLPDVPASIFEGEAELALVIGRRASHVKAAQAMKHVFGYTCFIDG
                     SARDLPPAGNTFYQMKSRDTFAPMGPFIVTADEIRDPHKLQIRLWVNGTLKQDFNTSD
                     MAHRIPRCIEWVSSIHALEPGDVLATGTNHRGLSAFQDGDRIELEVEKVGRLRVKVRD
                     RLKRTWSRDTRLECEQKGIKGRAPQLSGKYAPARSPAPAPAA"
     gene            complement(5592..5798)
                     /locus_tag="VI319_11485"
     CDS             complement(5592..5798)
                     /locus_tag="VI319_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864510.1"
                     /translation="MTPLDRPLKRELDIGGVKYTLTLGPEGLKLTPKGARKGIEISWH
                     SLVSGDAALAAALNASISQNKDTK"
     gene            complement(5795..7444)
                     /locus_tag="VI319_11490"
     CDS             complement(5795..7444)
                     /locus_tag="VI319_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6864511.1"
                     /translation="MSTETFDYVIVGGGTAGCVLANRLSADPDVRVLLLEAGGRDDWR
                     WIHIPIGYIYCIGNPRTDWCFKTEACDGLNGRSILYARGRVLGGCSSINAMIYMRGQK
                     RDYDEWAKLAGDPSWSWESVLPVFRRSEDHWRGASVWHGSGGEWRVEQQRLSWEILDA
                     FQAAAEECGIPRTEDFNRGDNEGSGKFEVNQRRGVRVSAAKAFLRPAMQRPNLEVRTD
                     STVQYLRMDGRRVTGVAYLRGGDQQYAEARAETILAAGAIGSPQVLQLSGIGPAGLLG
                     AHGIPVRHELPGIGENLQDHLQLRMAFKVSNARTANELVHSWTGKARMALEYAIFRSG
                     PLSMAPSQLGVFTRSSREHATPNLEYHVQPLSLERFGEELHEFPAITASVANLRPTSR
                     GWVRIASADPRAAPAINPHYLSTPEDRKVAADALRLTRRIARAKALERFAPEEFMPGR
                     QHDSDEELARAAGDVGTTIFHPVGTCRMGPASDRASVVDARLRVHGIGGLRVIDASIM
                     PTITSGNTNSPTVMIAERGAEMILADRKAAARREQVARSPA"
     gene            7520..8032
                     /locus_tag="VI319_11495"
     CDS             7520..8032
                     /locus_tag="VI319_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HEY6864512.1"
                     /translation="MTELKAVNLAEVSETLKHGVKPVSILWQAPDTIAFVARGRKGRS
                     EFHADPSDEVMYMIKGEMNLHYLTPGGEEKVAVIREGEIIYCPAGVKHSPRFSPDAFL
                     LVLERKRRPEEKDRFFWYCARCGAELYQAVRHVADYREDPVSRVYEEFYADDSHRSCA
                     KCGHVTPRPA"
     gene            8046..9176
                     /locus_tag="VI319_11500"
     CDS             8046..9176
                     /locus_tag="VI319_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY6864513.1"
                     /translation="MTRIAKVVLLTAVVFVVVWMGARLYRQAANDEPATAAAPAAVAP
                     APPAQEPPVVAQGRLSDWEKSVTGLFKAAAPSVAYITTEARRFDPLSGVELAQGAGSG
                     FVWDTDGHIVTNFHVIEDADTVYVQLDVGDPIPAKVIGGSPDYDIAVVRLSEVPKGLR
                     PLPVGDSKTLQVGQSTFAIGNPFGLSRTLTTGIVSALERRLPTSRGREVRGVIQTDAA
                     INPGNSGGPLLDSSGRLIGVNSAIYSESGTSAGIGFAIPVDLVKRVVPEIIRTGRAPR
                     PGIGIAAADERIASQLGVQGVMVLGVARGSPAQKAGIQPWDLRRRRVGDVIVALDGKP
                     VATLSDFAAMLDDVGIGKEVTLTVRRGDDEREVKARTIDLQD"
     gene            9218..9880
                     /locus_tag="VI319_11505"
     CDS             9218..9880
                     /locus_tag="VI319_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY6864514.1"
                     /translation="MSQESQYVSQGIGPLARLGEDDLRKLAPHGNVRAYPKNAVIINE
                     GDETDSLYVLLAGRVKVFVSDEDGKEAVLSTSREGSYFGELVIDGGKRSASVMAMEPC
                     RCFVIPMSDLQGLLESNPTFALHMIHLLIAKTRSLVKQVSDLALKDVYGRFAKFVDES
                     AVERDGIRVVPERLTQTDIAARIGGSREMVNRIVRDLTAGGYISVDARQITIHKRLPA
                     HW"
     gene            10026..10232
                     /locus_tag="VI319_11510"
     CDS             10026..10232
                     /locus_tag="VI319_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864515.1"
                     /translation="MSKRENPVVDTLLAAAIAVGGICVVAMMVPAGSASSAAVASAAS
                     VEAGYLPAQVANQAKEIEPLPAQF"
     gene            10323..10862
                     /locus_tag="VI319_11515"
     CDS             10323..10862
                     /locus_tag="VI319_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY6864516.1"
                     /translation="MRPVSWDGLAKAFHWVMAVLIFVQIALGLMAANWHLSPTQIRLF
                     FWHKSIGMLILALLALRLAWRLAGRAPALPADMPAWERAAAHASHALLYLLMVALPVT
                     GWLVNSAANIPFRVFYLVPLPPIVAPDKALADDLAWVHRGLFVLLALVLVAHIGAALR
                     HHFVKRDDVLARMLPWRMQ"
     gene            10859..11410
                     /locus_tag="VI319_11520"
     CDS             10859..11410
                     /locus_tag="VI319_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY6864517.1"
                     /translation="MNKALALVALLAAAAAAHAADWKSVPAGSKLEIVASFEKTPVPG
                     EFKQFDARLKGFDPQKGGGSLDVVIQTASADMGVADVNREIRGKDWFDMAAHPKAEFH
                     SSEIRREGNRWIARGTLALKGAKQPVDVPFAWTPSGDGATMEGEFTVKRGAFGIGAGE
                     WASSDTIGADVKVRFKVRMAKGG"
     gene            11410..12102
                     /locus_tag="VI319_11525"
     CDS             11410..12102
                     /locus_tag="VI319_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY6864518.1"
                     /translation="MRRILVAAVVLVLAGCAPSPVREPAKAPAEAPKDFPESHYRELL
                     AAGKPVYRVDPKESVVVIEVRRAGKFANLGHDHVVASRNLRGWIAPEEGRADLYVALD
                     ALTVDEPELRQESGFETQPSETDVAGTRANMRDKVLEVDRFPYALVHATVKDGDRALR
                     LSITLHGTTRSFDVPAKVDADAERVEASGRLSFDQSAFGITPYSVLGGAVAVRDRLDL
                     RFDVRARRAGAD"
     gene            12117..13271
                     /locus_tag="VI319_11530"
     CDS             12117..13271
                     /locus_tag="VI319_11530"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6864519.1"
                     /translation="MARAWTDPFPGNFMWSNATLVCRGMAPYGAVALGEIERVCERLA
                     ARGPTDPDAWWQEWGAMAEKNERLADAAAAEGRDATAGRHYLRAGNYYYTGERFVPPG
                     ERKLAIYKQALRCYREGLALRHPEIETVEVPYEGQSLPAYFMRSENALGRAPTVVLFD
                     GMDNCKEMSVLFAGLEFAKRGFHTLAIDGPGQGESLRLRQLHARHDYEAAGIAAYEFV
                     AARRDVDARRVAIMGYSFGGYYAPRIAAFEPRYAACVCLGALHWDLHAWQARIKAQLR
                     DDPKKSAQSNFQFQWILGLSDSDEALERAKRFSLAGVAGRIECPVLITHGQDDRVVPV
                     EAAHQLYAALRTGKKKLRIFTADEGGAEHCQVDDRQQGIDFIADWLSETL"
     gene            13268..14251
                     /locus_tag="VI319_11535"
     CDS             13268..14251
                     /locus_tag="VI319_11535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864520.1"
                     /translation="MKSRSFASLRMTAAAAVLCLLAASSFAQGYPSKPVRLVVPFPAG
                     ESVDATARLVSQSWSSALGAQVVVDNRGGAGGTIGSEAVAKSAPDGYTLLWGNVGPLA
                     IGPSLYKKLGYDVAKDFAPVSLVATLPFVLFASPQLKADSVPELVAYAKAHPGEINFG
                     STGVGSGLHLIAELFKSAAGIDIVHVPYKGVAQALPEMMAGKVQIAFNTIPAFLPHVK
                     AGRLKALAITAEQRSPLLPQVPTMPEAGQPAVLGGSWHAVVAPAGVPKDVIVKLNRTL
                     VASVEEHEVYKTLLAQGADPVGSTPEELAAFMRDEAAKWGRVIRASGARAE"
     gene            14248..15087
                     /locus_tag="VI319_11540"
     CDS             14248..15087
                     /locus_tag="VI319_11540"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY6864521.1"
                     /translation="MSAVRRVAVLALAVAPVAAIAQEDPATTTETRWRVAVGAGVLSA
                     PKFPGSDKQRTLALPVVLASLSYGRFFIGGDPAAGSAGGIGMRLYTDPHWRFAAGLSA
                     DLGHRKESDDPRLTGLGDVDRTASAFVSAAYVAGPVTTRLRVASDILGHDQGTLARLD
                     VLARARASERVTVFAGPGVTWADRRYTQTFFGVTADQSARSGLPQFDAGAGWNSARFS
                     AGAIWRIDSRWSASAFAAVARLQGDAVDSPITEDRTQYYAGVFLTYRFGNAPARVETA
                     APL"
     gene            complement(15084..17315)
                     /locus_tag="VI319_11545"
     CDS             complement(15084..17315)
                     /locus_tag="VI319_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY6864522.1"
                     /translation="MSPTLSRRLCTPLLAGLAALLIAAPPAQARVTRIVIDQPATPLS
                     STGQTIAYEQISGRAFGELDPADPLNAIIQDIDLGKDLDGKVRYVASFVLTKPVDMSQ
                     ASGMMWHDVPNRGTPITIVVAERNLGDVGLASAWQGDNAAINASNGTAVRPTMLVGGR
                     HFVQVPVAKNPDGSTVTGLVFGRIVNRSGLGAQPLIVQTNPVPYLPFTQDTAQATLVS
                     RDHETMEGVVTGETPIASADWKFCGGGTFDAPLPLTALPVQICLKGGFDVHKLYQVVY
                     TAKDPYVLGVGFAAWRDVGDFFKYAAQDDVGTANPVAGRIRWSIARGVSQSGNFLRGW
                     LHLGFNQDEARRQVHDGMWPIIAGRRIALNFRWAQPDGVLELYQAGSEGPQWWVKYED
                     KVRDLPKRGILDRCQDSKTCPKVIEHFGSAEVWALKLTPEWVGTDAKKDIRLPDNVRR
                     YYIPSSTHGGGAGGFNTSLPGVGLPTVGANCPGNNYGTGILPANPMPHTETVNAIRVH
                     FRNWVMKNVDPPPSVWPRMNPNAHEGDDDDHGQGDRGPRAPDLVNSNQTAMGFPKIPP
                     AAAFPGWRATAPEAGFINPVLDYDWGPQFDPSDATGVPTNVPPPIKQVIRTLAPRTDA
                     DGNEQGGVPVVLQLAPLGTYLGWNITDGRNPASAGGFRPFHADQLCDYVGGMIPFAKT
                     KAQRLATGDPRPSLEERYTDHNGYVNAVRAAAAKAVAAKFLLQEDADKLIQQAIDSAV
                     LNP"
     gene            17451..>17873
                     /locus_tag="VI319_11550"
     CDS             17451..>17873
                     /locus_tag="VI319_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY6864523.1"
                     /translation="MPTIAPQQKTAVVLGTATPGGGFPAYGDAYASTINDTDPTLSIE
                     PRNTRGSTENIPLLEAGKIDIGLATGEVTYEALAGIGRAKAELRIVNAMYASFGMFAV
                     RADSPARTIADLKGKPIAWGASGSGFVVLARYVMDGLGL"
BASE COUNT         2537 a         6261 c         6430 g         2645 t
ORIGIN      
        1 ggcgtcggca cggtccctct cctgcatcgg ctcggcgatg cgccacggca gcgcggcgag
       61 ctcgaccacg ccgacgagcg cgatgcgctt ctccgcgagc agcgcggcgg cggccttgcc
      121 gaggtccggt gcggcgcgca cctcgccgac gtggcaggtc tcctcgatcc acgcgctcat
      181 gcgcttggag aacgcggtga gcagcaccgg cgcgcccgac gcgaacaccg cgagcagcgc
      241 gtcgttccag tacggcacga aatgcgtgag ccaggcgacg gccgcggggc gcgcgaacga
      301 ggtgtagacg agcacgcagt cgagcgcctg cttgttcatc gccgcctgca gcttcgccac
      361 gcgcgcgtcg agcgccgagg gcggcacctc gtcgcgcgac cacgcgatca ggccgtgcct
      421 cacgcgggaa cctcgaacaa cgcgcgagcc gtgcgcgtca gcacctcgca tccgctctcg
      481 gtgaccacgg cgctgtcgcc gtgcacgaag tagccgacct ccgggtgata ggtgttgggg
      541 tgcacgatca gcgtcattcc cggccggaac tcgagcgcga cgtcctcgag cagcatcgga
      601 cgcgagtcga cgtagaggcc gaggccgtgg ccgcgcacgc gcgtgtactc ggaggtgacg
      661 tagtcgccca ggccctcgcg gcggaagatc ccgttctccg cgcgcgccac gtcgcccgcg
      721 gtggcgccgg gcgcgagcgc ggcgagcccc gcccgcagcg cctcgtcgta gacggcgaag
      781 gcgcgccgct gctccccgct cgcgcgcccg atcacgaggg tgcggcagat ctgcgcgtag
      841 tacccgtcga cgcagggcgt gagctcggtg gtgacgaggt cgccttcccg gaggcgccgc
      901 tcgcccgggg gatgcatgcc gcgcacctcg cgcccgcccg acgcgaggat ctggaaattc
      961 tccgggcagc cgcgcgagcg gaagaacgcc tcgacctcgg ccaccggctc gtactcggcg
     1021 cggcccgcgc gagcggcctc gcggaacacg cgatagccct cgtcggcgag cgccgcggcc
     1081 ttgcgcaccg cgtcgatctc gcgcggcgat ttcaccagca tcaggcggtc gagcagcgcc
     1141 gtgccttcgg cgatcgtctt gcggaacggt ccttttgcga ggcggcaggg cacggccgcg
     1201 agcggcgcca gggcgacgcg cttcacgatc agcgcgccga tcgcgcgctc cgcctcggtg
     1261 atcgccgagc gcgaggcgct caccgcgacg tcgggcagct cccgggcgaa gcgcgcgcct
     1321 tccgccggcg agtcgacgaa caggcgcgcc gcgccgccgc gctcgaggat cgcgatcgcc
     1381 cggccctcga gcggcgtcgc gtcgaccgcg tagcgcaggt agtcgctgcg ccaggggtcg
     1441 ccggcgacga cgagcgcatc caggcccgcc tcggcgagcg ctgcgtcgat gcgcgcacgg
     1501 cgctcctcgc gcaaggcccg ggacgacggc gacatggcta gagctccggc ttgatgcggc
     1561 cggtctcgac gatcttcttg tagcgcgccg actccgagcg caggaacgcc gcggtgtcgg
     1621 cgcgagacag gccggtgggc aggtgcccgg cgtcgcggat gcgcttgcgc accgccggat
     1681 cggcgagcgc ggcatcgacc tcgcgcgcga ggcgctcgac cgtgcgcgcg tcggtgccgc
     1741 gcggcgcgga cagcacccac cagttgccga agacgaggtc cgggaacccg gcctcgaccg
     1801 tcgtcgggac atcgggcagc tcgggcagcc gctcgcgtcc catcaccgcg agcacgcgca
     1861 cgcggccggt cttgagcgcg ccgagcacgg tcgtgatggt cgggaaggtg acctggatgt
     1921 cgttcgcgag cagcgcctgc accatcggcg cgatgccctt gtacggcacg tagaccatgg
     1981 cgttgccggt gagctgcgag aacagcgcgc ccgtgaggtg cgacggcgac ccggtgccgg
     2041 gcgcgccgta attgagcttg ccgggattcg cgcgcacggt ttgctcgagc tccttcaacg
     2101 agcgcgccgg gacctcggca ttgatcaccg cgagcagcgg cgcctcggcg agcatcgcaa
     2161 tcggctcgag cgccgcgagc gggtcgaagc cgaggttcgg gaacaggtgc tggttcaccg
     2221 cgtagttcgc cgtcggcgcg agcagcagcg tgtagccgtc gggcgccgcg cgcgcgacct
     2281 cggcggtgcc gatgttgccg gccgcgcccg gcttgttgtc gaccagcagg cgctggccga
     2341 gcttcgactc cacctgcggg gcgatcgcgc gcagcagcac ctcgccgacc gagccggcgg
     2401 tgttcgggac gacgacgtgg atcggcttcg agggccactg ctgtgcggaa gccgcgcacg
     2461 acgcaaagat cagggcaccg agaagcagcc gtcgcaacat cagtcggccc tcaaatgatt
     2521 cctgccgatg atctcgcccc agcgctcgta gtcgcgccgc accacggcgt tgaattcgtc
     2581 gcccgagagg cccgagggct cgacgccgag ctgccggaag cgctcgccga ccgcgggcag
     2641 cttcagcacc tcgcgaatgt ccgacgcgat ctgctcgagc accggcttcg gcgtcgcccc
     2701 cggcgcgaag atgccgtgcc agctgccgat gccgaggccg gggaagagcc ggtcgaaggg
     2761 aaccgcgccc ggcagctgct cgagcggctc cgatccggtc accaggaccg cgcgcgcctt
     2821 gcccgacttg accagcggca ccgtcgagac cgtcacgtcg agcatcatcg ggatctcgcc
     2881 cgccatcacg gcctgcagcg cgggcgccgc gcccttgaag ggaatgtgcg tgagatcgat
     2941 gcccgcggcc gacttgagca gctccatgta gagatgctgt cccgagccgt tgcccgcgct
     3001 cgcgtagttc agcttgccgg gcgagcgctt cgcgcgcccg atgaggtcgt cgatcgactt
     3061 gatgcccgac gcggggttca ccacgagcgc gtaagtgagc agcgtcgtct gcgtcacgcc
     3121 ggtcaggtcg tggagcacgt cgtagggcac cttctgcatc tgcggcagcg tgaccagcgg
     3181 cgtcgagctg atgatcatca gcgtgtagcc gtcgggcgcg ctcttcacca cggactccgg
     3241 gccgatggtg ctgccgggct tgttctcgat caccacgggc tcgcgccagc gctccgacag
     3301 gtgctggccg atcagccgcc cggagatgtc gagcgggccg cccggcggga agggcaccac
     3361 cagccggacc ggcctcgacg ggaacgtgtc ggaaagcgcg ggcggggcga gcgctgcgga
     3421 tacgagggcg aaaaacaggg cgacgacgcg cttcatcggg ggcgtttcct ccgtgactga
     3481 ttatattagg gaagttgcgc agccgccgct gcccttaagg tttgcataag gcgcgccgtc
     3541 gtaacatggt catctccatg gaatcttccg tctggcgcga ccgggtgctg cgctgggccg
     3601 gcgcgttcgt ggcgacggcc gtggcgctgg tgctgcgcga cacgctcgag cctctgctcg
     3661 gcggctcggt gccgttctcg acgctgtacg ccgcggtcgc ggtggtggtg ctcatcggcg
     3721 gctacgggcc cgcgctcgcg gcgatgatcg gcggctacgt cgccgccgag tacttcttcg
     3781 tgcagccggt gcataccgcg ttcgtgagcg ctccgcccga tccgctgcgg ctcgcgctgt
     3841 acacgctctc gtgcctgatc atcatcggct tcggcggcgg catgcgctcg gcgcggcggc
     3901 gcctggagcg cgaggtcgcg cggcgcaagc gcctcgagga gaagctgcgc gaggccgaca
     3961 agcgcaagga cgtcttcctc gcgaccgtcg cgcacgagct caagcatccg ctcgcgccga
     4021 tccgcaacgt cctcgaggtg ctcgagctca aggacctgcc cgaccgggag ctgcgctggg
     4081 cgcgcggcat catcgaccgg caggtgaaga acctcgcgcg cctcgtcgac gatctgctcg
     4141 acgtgtcgcg catcgtgcgc gggaacatgg agctgcgccg cgagccgctc gcggtgtcgc
     4201 gcatcgtcgc cgacgcggtc gaggtctgcc gcccgcccgc gcaggcgcag cggcacgagc
     4261 tcgccgtggc cttgccgcgc cagccgctct gggtcgaggg cgacctcacg cgcctcacgc
     4321 aggtggtcgt gaatctgctc gacaacgcgg tcaagtacac gccgccgggc gggcacgtcg
     4381 cggtctcggc cgagcgagac ggcgacgcgg cgctcatcgt ggtccgcgac gacggcatcg
     4441 gcatcccgga gagcatgctc gagcgcgtgt tcgagccctt cgcgcgctcc gacgaggcga
     4501 gggcgcgcgc ggcgggcggc atggggatcg gcctctcggt cgtgcgcgac ctggtcgagc
     4561 tgcacggcgg cggcatcgag gcgcgcagcg ccgggcgcga ccgcggcagc gagttccgcg
     4621 tgcggctgcc gctgtgcgct acgccgccgg cgcgggagcc ggggagcgcg ccggggcgta
     4681 ctttcccgag agctgaggcg cccgcccctt gatgcctttc tgctcgcact cgaggcgcgt
     4741 gtcgcgcgac caggtgcgct tcagccggtc cctcaccttg acgcgcaggc gcccgacctt
     4801 ctccacctcg agctcgatgc ggtcgccgtc ctgaaaggcg gacaggccgc gatggttggt
     4861 gccggtcgcg agcacgtcgc ccggctcgag cgcgtggatc gacgacaccc actcgatgca
     4921 gcgcgggatc ctgtgcgcca tgtcgctcgt attgaagtcc tgcttgagcg tcccgttcac
     4981 ccagagccgg atctgcagct tgtgcggatc tcggatctcg tccgcggtca cgatgaacgg
     5041 ccccatgggc gcgaacgtgt cgcgcgactt catctggtag aaggtgttgc ccgcgggcgg
     5101 caggtcgcgc gccgagccgt cgatgaagca ggtatagccg aagacatgct tcatcgcctg
     5161 cgcggccttc acgtggctcg cgcgccggcc gatgaccagc gcgagctcgg cctcgccttc
     5221 gaagatcgac gccggcacgt cgggcaggac catggtgtcg ccgtcgccga tcaccgcgcc
     5281 cggcgacttg tggaaggcgt tgatcggcgc gggctcggag cgcgtgccgt cctccatgta
     5341 gttcaccgcc atgcagtcga tgttgaccgg cctcggcagc ggcgagcgca ggcgcacctt
     5401 cttcagcggg atgccgcgcc ccgccgcggc cgccttctcg agcttcgcgc gatatttcgg
     5461 aaagtcggcg atcagcgcag agatcaggtc gtgcggcccc gtgtgcggaa cactcttcac
     5521 ggccttcgag acgtcgacga cggcgtcgcc tttgacgatc ccgagcctga aatcgtcgaa
     5581 gtagcagatc ttcatttcgt atccttgttt tgtgagatcg aagcgttcag cgctgcggcc
     5641 agggccgcgt cgccgctgac gagggagtgc cacgagatct cgatgccctt gcgcgcgccc
     5701 ttgggcgtga gcttcaggcc ctccggcccg agcgtcagcg tgtacttgac gccgccgatg
     5761 tcgagctcgc gcttgagcgg cctgtcgagg ggcgtcatgc cgggctccgc gcgacctgct
     5821 cgcgccgcgc cgccgccttg cggtcggcga ggatcatctc ggcgccgcgc tcggcgatca
     5881 tgacggtggg cgagttggtg ttccccgagg tgatcgtggg catgatcgac gcgtcgatca
     5941 cgcgcaggcc gccgatgccg tgcacgcgca ggcgcgcgtc gacgacgctg gcgcggtccg
     6001 acgccggccc catgcggcag gtgccgaccg ggtggaagat ggtcgtgccg acgtcgcccg
     6061 cggcgcgcgc cagctcctcg tcgctgtcgt gctggcgtcc cggcatgaac tcctcgggcg
     6121 cgaagcgctc gagcgccttc gcgcgcgcga tgcggcgcgt gagccgcagc gcgtcggccg
     6181 cgaccttgcg gtcctcgggc gtcgacaggt aatgcgggtt gatggcgggc gcggcgcgcg
     6241 ggtcggcgct cgcgatccgc acccagccgc ggctcgtcgg gcgcaggttc gcgaccgacg
     6301 cggtgatggc ggggaactcg tgcagctcct cgccgaagcg ctcgagcgag agcggctgca
     6361 cgtggtactc gaggttgggg gtcgcgtgct cgcgcgagga gcgcgtgaag acgccgagct
     6421 gcgacggcgc catggacagc gggccggagc ggaagatcgc gtactcgagc gccatccggg
     6481 ccttgccggt ccagctgtgc acgagctcgt tcgccgtgcg cgcgttgctc accttgaagg
     6541 ccatgcgcag ctgcaggtgg tcctgcaggt tctcgccgat cccgggcagc tcgtggcgca
     6601 ccgggatgcc gtgcgcgccg agcagtcccg ccgggccgat gcccgagagc tgcagcacct
     6661 gcgggctgcc gatcgcgccc gcggcgagga tggtctcggc gcgcgcctcg gcatactgct
     6721 gatccccgcc gcgcaggtac gcgacgcccg tgacccgcct gccgtccatg cgcaggtact
     6781 gcaccgtgga gtcggtgcgc acctcgaggt tcggccgctg catcgccggc cgcaggaagg
     6841 ccttggccgc ggacacgcgc acgccgcggc gctggttcac ctcgaatttg ccgctgccct
     6901 cgttgtcgcc gcggttgaag tcctcggtgc gcgggatgcc gcactcctcg gccgcggcct
     6961 ggaaggcgtc gaggatctcc cacgagaggc gctgctgctc gactcgccac tcgccgcccg
     7021 agccgtgcca caccgaggcg ccgcgccagt ggtcctcgga ccggcggaac accggcagca
     7081 cgctctccca ggaccacgac gggtcgcccg ccagcttcgc ccactcgtcg tagtcgcgct
     7141 tctggccgcg catgtagatc atcgcgttga tcgacgagca gccgccgagc acgcgcccgc
     7201 gcgcgtacag gatcgagcgg ccgttcagcc cgtcgcaggc ctcggtcttg aagcaccagt
     7261 cggtgcgcgg attgccgatg cagtagatgt agccgatcgg gatgtggatc cagcgccagt
     7321 cgtcgcgccc gcccgcctcg agcagcagca cgcgcacgtc gggatcggcc gagaggcggt
     7381 tggcgagcac gcagccggcg gtgccgccgc cgacgatgac gtagtcgaag gtctcggtgc
     7441 tcatgggttc ggaaactcta gcagccttcg cgccgctccg tcatccggat taatatgacg
     7501 cctcccggag acacccccga tgaccgagct caaagcagtc aacctcgccg aagtctcgga
     7561 gaccctcaag cacggcgtga agccggtgag catcctgtgg caggcgccgg acaccatcgc
     7621 cttcgtcgcg cgcggccgca aaggccgaag cgagttccac gccgacccga gcgacgaggt
     7681 catgtacatg atcaagggcg agatgaacct gcactacctc acgcccgggg gcgaggagaa
     7741 ggtcgccgtc atccgcgagg gcgagatcat ctactgcccg gcgggcgtga agcactcgcc
     7801 gcgattctcg cccgacgcct tcctgctcgt gctcgagcgc aagcgccggc ccgaggagaa
     7861 ggaccgcttc ttctggtact gcgcgcgctg cggcgcggag ctgtaccagg cggtgcgcca
     7921 cgtcgccgac taccgcgagg acccggtgtc gcgcgtctac gaggagttct acgccgacga
     7981 ttcgcaccgc agctgcgcga agtgcggcca cgtgacgccg cggcccgcgt gacccgggga
     8041 cgcccgtgac gcgcatcgcg aaggtcgtcc tgctcaccgc ggtggtgttc gtcgtcgtct
     8101 ggatgggcgc gcgcctgtac cgccaggccg ccaacgacga gcccgcgacg gcggccgccc
     8161 cggccgcggt cgccccggcg ccgccggcgc aggagccgcc ggtggtggcg cagggccggc
     8221 tctccgactg ggagaagagc gtgaccgggc tcttcaaggc cgccgctccg tcggtcgcgt
     8281 acatcacgac cgaggcgcgc cgcttcgatc cgctctccgg cgtcgagctg gcgcagggcg
     8341 cgggctcggg cttcgtctgg gacaccgatg gccacatcgt caccaatttc cacgtgatcg
     8401 aggacgccga caccgtatac gtgcagctcg acgtcggcga cccgatcccg gcgaaggtga
     8461 tcggcggctc gcccgattac gacatcgcgg tggtgcgcct ttcggaggtg cccaaggggc
     8521 tgcggccgct ccccgtcggc gactcgaaga cgctgcaggt ggggcagtcg accttcgcca
     8581 tcggcaaccc gttcgggctg tcgcgcacgc tgaccacggg gatcgtcagc gcgcttgagc
     8641 ggcgcctgcc gacgtcgcgc gggcgcgagg tgcgcggcgt gatccagacc gacgccgcga
     8701 tcaacccggg gaactcgggc gggccgctcc tcgacagctc ggggcggctg atcggcgtga
     8761 acagcgcgat ctattccgaa tccgggacgt cggccggcat cggcttcgcg atcccggtcg
     8821 acctggtcaa gcgcgtcgtg ccggagatca tccggaccgg ccgcgcgccg cgtcccggga
     8881 tcgggatcgc cgcggccgac gagcgcatcg cgtcgcagct cggcgtccag ggcgtgatgg
     8941 tgctcggcgt cgcgcgcggc tcgcccgcgc agaaggccgg aatccagccc tgggacctgc
     9001 gccgccgccg cgtcggcgac gtgatcgtcg cgctcgacgg caagccggtc gcgacgctgt
     9061 cggacttcgc cgcgatgctc gacgacgtcg ggatcggcaa ggaagtgacg ctgacggtgc
     9121 gccgcggcga cgacgagcgc gaggtcaagg cgcgcacgat cgacctgcag gactgaggca
     9181 taatccgctt cgcgcgtctg tcatggggga gagcgccatg tcgcaggagt cccagtacgt
     9241 ctcccagggg atcggacccc tggcccgtct cggcgaagac gatctgcgca agctcgcgcc
     9301 ccacggcaac gtgcgcgcgt atcccaagaa cgcggtgatc atcaacgagg gcgacgagac
     9361 cgattcgctc tacgtcctgc tcgccgggcg ggtcaaggtg ttcgtgagcg acgaggacgg
     9421 caaggaagcg gtgctcagca cctcgcgcga gggcagctac ttcggcgagc tcgtgatcga
     9481 cggcggcaag cgctccgcct cggtcatggc gatggagccc tgccgctgct tcgtgatccc
     9541 gatgagcgat ctccaggggc tgctcgagag caaccccacg ttcgcgctcc acatgatcca
     9601 cctcctcatc gccaagacgc gcagcctggt caagcaggtg agcgacctcg cgctgaagga
     9661 cgtgtacggc cgcttcgcca agttcgtcga cgagagcgcg gtcgagcgcg acggcatccg
     9721 cgtggtgccc gagcgcctga cgcagaccga catcgccgcg cgcatcggcg gctcgcgcga
     9781 gatggtgaac cgcatcgtcc gcgacctcac cgcgggcggc tacatctccg tcgacgcccg
     9841 gcagatcacg atccacaagc ggctgccggc gcactggtag ccctggtaat acggcacagg
     9901 gccgcggcgc cggaccgcac ggcgcgcgag tggcgcgcga cggcgagcgc gaaggtcagc
     9961 cgatcctaaa gtggtctccg tcgggaggcg atccccgccg cccgaaagac aaggaggaga
    10021 ccgcaatgag caagcgcgaa aatcccgtcg tcgacaccct gcttgccgcc gcgatcgcgg
    10081 tcggcggcat ctgcgtcgtg gccatgatgg tgccggcggg ctcggcgtcc tcggccgccg
    10141 tggcgagcgc cgcgtccgtc gaggccggct atctcccggc ccaggtggcg aaccaggcga
    10201 aggagatcga gccgctgccc gcgcagttct gaagcggcgg acgagcgccg tcgaggggcc
    10261 cgaacgggcc cctttctttt tttgcggcta caatcggcgg cgtcccccgg aaaggaaatc
    10321 gcgtgcggcc tgtgtcctgg gacggtctcg ccaaggcgtt ccactgggtg atggccgtgc
    10381 tgatcttcgt ccagatcgcg ctcggcctga tggcggcgaa ctggcacttg tcgccgacgc
    10441 agatccggct gttcttctgg cacaaatcga tcgggatgct gatcctcgcg ctgctcgcgc
    10501 tgcggctggc gtggcggctc gcgggccgcg cgccggcgct gcccgccgac atgcccgcgt
    10561 gggagcgcgc cgcggcgcac gcgagccacg cgctgctcta tctgctgatg gtcgcgctgc
    10621 cggtcacggg ctggctcgtc aattcggccg cgaacatccc gttccgcgtc ttctacctcg
    10681 tgccgctgcc gccgatcgtc gcgcccgaca aggcgctcgc ggacgatctc gcctgggtgc
    10741 accgcgggct gttcgtgctg ctcgcgctcg tgctcgtcgc gcacatcggc gcggcgctgc
    10801 gccaccattt cgtgaagcgc gacgacgtgc tcgcgcgcat gctgccgtgg agaatgcaat
    10861 gaacaaggcc ctggcgctcg tcgcgctcct cgcggcggcc gcggccgcgc acgccgccga
    10921 ctggaagtcg gtcccggccg gaagcaagct cgagattgtc gcgagcttcg agaagacgcc
    10981 tgtccccggc gagttcaagc agttcgacgc gcgcctcaag ggcttcgacc cgcagaaggg
    11041 cggcggcagc ctcgacgtcg tcatccagac ggcgagcgcc gacatgggcg tggccgacgt
    11101 caaccgcgag atccgcggca aggactggtt cgacatggcc gcgcacccga aggcggagtt
    11161 ccattcgagc gagatccgcc gcgagggcaa ccgctggatc gcgcgcggca cgctcgcgct
    11221 gaaaggcgcg aagcagccgg tcgacgtgcc gttcgcatgg acgccctcgg gcgacggcgc
    11281 gacgatggaa ggcgagttca cggtgaagcg cggcgccttc ggcatcggcg ccggcgaatg
    11341 ggcgtcgagc gacaccatcg gcgccgatgt caaggtccgg ttcaaggtcc ggatggcgaa
    11401 gggcggctga tgcgccggat cctcgtcgcg gcggtcgtgc tcgtgctggc gggctgcgcg
    11461 ccctcgccgg tgcgcgagcc cgcgaaggcg ccggcggaag cgccgaagga ctttcccgag
    11521 tcgcactacc gcgagctgct cgcggcgggc aagcccgtct accgcgtcga tccgaaggaa
    11581 tcggtcgtcg tcatcgaagt gcgccgcgcg gggaaattcg ccaacctcgg ccacgaccac
    11641 gtcgtcgcca gccgcaacct gcgcggctgg atcgcgccgg aggagggccg cgccgacctc
    11701 tacgtggcgc tcgacgcgct gacggtcgac gagcccgagc tgcgccagga atcgggattc
    11761 gagacgcagc cttccgagac cgacgtcgcg ggcacgcgcg ccaacatgcg cgacaaggtg
    11821 ctcgaggtcg acaggttccc ctacgccctg gtgcacgcga ccgtgaagga cggcgaccgg
    11881 gcgctgcggt tgtcgatcac gctgcacggg acgacgcgct cgttcgacgt cccggcgaaa
    11941 gtggacgcgg acgccgagcg cgtcgaggcg agcgggcgcc tctccttcga ccagagcgcc
    12001 ttcggcatca cgccgtactc ggtcctcggc ggagccgtcg ccgtgcgcga ccggctcgac
    12061 ctgcgcttcg acgtgcgcgc gcgacgcgcg ggcgccgact agggccgaag ctcccgatgg
    12121 cacgcgcctg gaccgatccc ttccccggca acttcatgtg gtcgaacgcg accctcgtgt
    12181 gccgcggcat ggcgccttac ggcgcggtcg cgctcgggga gatcgagcgc gtgtgcgagc
    12241 gcctcgcggc gcgcgggccc accgacccgg acgcgtggtg gcaggagtgg ggcgcgatgg
    12301 ccgagaagaa cgagcgcctc gccgacgccg cggccgccga ggggcgcgac gcgaccgccg
    12361 gccgccacta cctgcgcgcg ggcaactact actacaccgg cgagcgcttc gtgccgcccg
    12421 gcgagcgcaa gctcgcgatc tacaagcagg cgctgcgctg ctaccgcgag ggcctggcgc
    12481 tgcggcatcc cgagatcgag acggtcgagg tgccgtacga agggcagtcg ctgcccgcgt
    12541 atttcatgcg ctcggagaac gcgctcggcc gcgcgccgac ggtcgtgctc ttcgacggca
    12601 tggacaactg caaggagatg agcgtcctgt tcgcgggcct ggagttcgcg aagcgcggct
    12661 tccacacgct cgcgatcgac ggccccgggc agggcgagtc gctgcggctg cgccagctcc
    12721 acgcgcgcca cgactacgag gcggcgggca tcgccgcgta cgagttcgtc gccgcgcgcc
    12781 gcgacgtcga cgcgaggcgc gtcgcgatca tgggctacag cttcggcggc tactacgcgc
    12841 cgcgcatcgc cgcgttcgag ccgcgctatg cggcgtgcgt gtgcctgggc gcgctgcact
    12901 gggacctgca cgcgtggcag gcgcggatca aggcgcagct cagggacgat ccgaagaaga
    12961 gcgcgcaatc caatttccag ttccagtgga tcctcgggtt gagcgactcg gacgaggcgc
    13021 tcgagcgcgc gaagcgcttc tcgctcgccg gcgtcgccgg gcgcatcgaa tgcccggtgc
    13081 tgatcacgca cggccaggac gaccgcgtcg tcccggtcga ggccgcgcac cagctgtacg
    13141 cggcgctgcg gaccgggaag aagaagctca ggatcttcac cgccgacgag ggcggcgccg
    13201 agcactgcca ggtcgacgac cggcagcagg ggatcgattt catcgcggac tggctttcgg
    13261 agacgttatg aaaagcagat ccttcgcttc gctcaggatg actgcggccg ccgccgttct
    13321 gtgcctgctc gccgcatcga gcttcgcgca ggggtatccg tcgaagccgg tgcgtctcgt
    13381 ggtcccgttc ccggccggcg agtcggtgga cgcgaccgcg cggctcgtgt cgcagtcgtg
    13441 gtcgtcggcg ctcggcgcgc aggtcgtggt cgacaaccgc ggcggcgcgg gcggcacgat
    13501 cggcagcgag gccgtggcga agtccgcgcc cgacggctac acgctgctct ggggcaacgt
    13561 cgggccgctc gcgatcgggc cctccctgta caagaagctc ggctacgacg tggcgaagga
    13621 tttcgcgccg gtgtcgctgg tcgcgacgct gccgttcgtg ctgttcgcga gtccgcagct
    13681 gaaggcggat tccgtccccg agctggtcgc gtacgcgaag gcgcaccccg gcgagatcaa
    13741 cttcggctcg accggcgtcg gctcggggct gcatctcatc gccgagctgt tcaagagcgc
    13801 ggccggcatc gacatcgtgc acgtgcccta caagggcgtc gcgcaggcgc tgcccgagat
    13861 gatggcgggc aaggtgcaga tcgcgttcaa caccatcccc gccttcctgc cgcacgtgaa
    13921 ggccggacgc ctgaaggcgc tcgcgatcac agccgagcag cgctcgccgc tgctgccgca
    13981 ggtgccgacg atgcccgagg cgggacagcc cgcggtgctc ggcgggtcct ggcacgcggt
    14041 cgtcgccccg gccggcgtgc cgaaggacgt gatcgtgaag ctcaatcgca cgctggtcgc
    14101 gagcgtcgag gagcacgagg tgtacaagac gcttctcgcg cagggcgccg acccggtcgg
    14161 cagcacgccc gaggagctcg ccgccttcat gcgcgacgag gctgcgaaat ggggacgcgt
    14221 catccgggcc tcgggcgcgc gcgcggaatg agcgccgtgc gccgcgtcgc ggttctcgct
    14281 ctcgcggtcg ctcccgtcgc tgccatcgcg caggaagatc cggcgacgac gacggaaacg
    14341 cgctggcgcg tcgccgtcgg cgccggcgtc ctcagcgcgc cgaaattccc ggggagcgac
    14401 aagcagcgca cgctcgcgct gccggtcgtc ctcgcgagcc tgagctacgg gcgtttcttc
    14461 atcggcggcg acccggccgc gggcagcgcg ggcggcatcg gcatgcggct gtacacggat
    14521 ccgcactggc ggttcgccgc ggggctctcg gccgatctcg ggcaccgaaa ggaatccgac
    14581 gacccgcggc tcaccggcct cggcgacgtc gaccgcaccg cgtcggcctt cgtctccgcc
    14641 gcgtacgtcg cgggcccggt gacgacgcgg ctgcgcgtcg cgagcgacat cctcggccac
    14701 gaccagggca cgctcgcccg cctcgacgtg ctggcacgcg cgcgggcgtc cgagcgcgtc
    14761 accgtgttcg ccggaccggg cgtgacctgg gccgaccggc gctacacgca gaccttcttc
    14821 ggcgtcaccg ccgatcagag cgcgcgctcg ggcctgccgc agttcgacgc gggcgccggc
    14881 tggaacagcg cgcgcttctc cgcgggcgcg atctggcgca tcgactcgcg ctggagcgcg
    14941 agcgccttcg cggcggtcgc gcggctgcag ggggacgccg tcgacagccc gatcaccgag
    15001 gatcgcacgc agtattacgc cggcgtgttc ctgacctatc ggttcgggaa cgcgccggcg
    15061 agagtcgaaa cggcggcgcc gctctaggga ttcagcaccg cgctgtcgat cgcctgctgg
    15121 atcagcttgt ccgcgtcctc ttgcagcagg aacttcgccg cgaccgcctt cgcggccgcc
    15181 gcgcgcaccg cgttgacgta gccgttgtga tcggtatagc gctcctcgag cgacgggcgc
    15241 gggtctccgg tcgccaggcg ctgggccttc gtcttcgcga acgggatcat tccgccgacg
    15301 tagtcgcaga gctggtccgc gtggaacgga cggaatccgc ccgccgaggc cgggttgcgc
    15361 ccgtcggtga tgttccagcc gagataggtg ccgagcggtg cgagctgcag cacgaccggc
    15421 acgccgccct gctcgttgcc gtcggcgtcg gtgcgcggcg cgagcgtccg gatcacctgc
    15481 ttgatcggcg gcggcacgtt ggtcggcacg ccggtcgcgt cgctcgggtc gaactgcgga
    15541 ccccagtcgt agtcgagcac cgggttgatg aagcccgcct cgggcgcggt cgcgcgccag
    15601 cccgggaacg cggccgcggg cgggatcttc gggaagccca tcgcggtctg gttcgagttg
    15661 accaggtccg gcgcgcgcgg tccgcggtcg ccctgaccgt ggtcgtcgtc gtccccttcg
    15721 tgcgcgttcg gattcatccg cggccacacg ctcggcggcg ggtcgacgtt cttcatcacc
    15781 cagttgcgga aatgcacgcg gatcgcgttc accgtctcgg tgtgcggcat cgggttcgcg
    15841 ggcaggatgc ccgtgccgta gttgttgccc gggcagttcg cgcccacggt cggcaggccg
    15901 acgccgggca ggctggtgtt gaagccgccc gcgccgccgc cgtgcgtgct gctcgggatg
    15961 taatagcggc gcacgttgtc cggcagccgg atgtccttct tcgcgtcggt gccgacccac
    16021 tccggcgtga gcttgagcgc ccacacctcg gccgagccga agtgctcgat caccttcgga
    16081 caggtcttcg agtcctggca gcggtcgagg atgccgcgct tcggcagatc gcgcaccttg
    16141 tcctcgtact tcacccacca ctgcggacct tcgctgccgg cctggtacag ctcgagcacg
    16201 ccgtcgggct gcgcccagcg gaagttcagc gcgatgcggc ggccggcgat gatcggccac
    16261 atgccgtcgt gcacctggcg gcgcgcctcg tcctggttga acccgaggtg cagccagccg
    16321 cgcaggaagt tgcccgactg cgacacgccg cgggcgatgc tccagcggat ccggccggcg
    16381 accgggttcg cggtgccgac gtcgtcctgc gccgcgtact tgaagaagtc gccgacgtcg
    16441 cgccaggcgg cgaaccccac gccgagcaca tacgggtcct tcgccgtata cacgacttgg
    16501 tagagcttgt gcacgtcgaa gccgcccttc aggcagatct gcaccggcag cgccgtgagc
    16561 ggcagcggcg cgtcgaacgt gccgccgccg cagaacttcc agtcggctga cgcgatcggc
    16621 gtctcgccgg tcaccacgcc ttccatggtc tcgtgatcgc gcgagaccag cgtcgcctgc
    16681 gccgtgtcct gcgtgaacgg caggtacggc acgggattcg tttgcacgat gagcggctga
    16741 gcgccgaggc cgctgcggtt gacgatgcgc ccgaaaacca ggccggtgac cgtcgagccg
    16801 tcggggttct tcgccaccgg cacctggacg aagtggcggc cgccgaccag catggtcggg
    16861 cgcaccgcgg tgccgttgct cgcgttgatg gcggcattgt cgccctgcca ggcgctcgcc
    16921 aggccgacgt cgcccaagtt gcgctccgcg accacgatcg tgatcggcgt gccgcggttg
    16981 ggaacgtcgt gccacatcat gccgctcgcc tgcgacatgt cgaccggctt ggtgagcacg
    17041 aacgaggcga cgtagcgcac cttgccgtcc aggtccttgc caagatcgat gtcctggatg
    17101 atcgcgttca gcggatcggc cggatcgagc tcgccgaagg cgcgccccga gatctgctcg
    17161 tacgcgatcg tctggccggt ggaggagagt ggcgtcgccg gctggtcgat cacgatgcgc
    17221 gtgacgcgcg cctgcgcggg cggagcggcg atgagcagcg cggcgaggcc cgcgagcagg
    17281 ggagtgcaaa ggcgacgaga cagcgtgggg gacatgtcgt ctccttggtt gattgttctg
    17341 ctgagaccgg cgactgccgg cgtggagaac gttagaccgg tcgcgtgctt tatgcaacac
    17401 gcatacccgc gtgctagagt cgcgcgctct gtgttccgga gggaagaacg atgccgacca
    17461 ttgcgccgca gcagaagacc gccgtcgtcc tcggcaccgc gacgcccggc ggcggctttc
    17521 ccgcctacgg cgacgcctac gcgtcgacga tcaacgacac ggacccgacg ctctccatcg
    17581 agccacgcaa caccaggggc agcaccgaga acatcccgct gctcgaggcg gggaagatcg
    17641 acatcggcct cgcgacgggc gaggtgacgt acgaggcgct cgcgggcatc ggccgggcga
    17701 aggccgagct gcgcatcgtc aacgcgatgt acgcgagctt cggcatgttc gcggtgcgcg
    17761 ccgacagccc ggcgcggacg atcgccgacc tcaaaggcaa gccgatcgcc tggggcgcgt
    17821 cgggctcggg cttcgtcgtg ctcgcgcgct acgtgatgga cggcctcggg ctc
//
LOCUS       DATDMW010000267         4894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_829280, whole genome shotgun sequence.
ACCESSION   DATDMW010000267 DATDMW010000000
VERSION     DATDMW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829280"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VI319_11555"
     CDS             complement(<1..1221)
                     /locus_tag="VI319_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6864524.1"
                     /translation="MRNFSKRVRRWVAGLAMLAACVAAPAQDNVIGLTLRDAEQQLAT
                     GNRDVRLARRALEGAQASTVSAGARPNPNLSLGVSQINPQVGVGGGPYRDKTVDSSVR
                     VDQLIERGDKRELRIAQAQRSEAAASQDYADVLRQQLLALRSAYYDLAFAQDKSAISR
                     DNIALFERTIEAAELRYKAGDIAAADVSRLRVDALRAQNDARSAEADRRRAQLALAYL
                     IGAEAQAAGLRATDPWPAPDSVDLPQVTDEMIDRRPDVRAARSRVEAAAGARDLARSL
                     RTRDVTVGLSYDHYPVNPGFAQGSGAGTGNTYGLFVSVPLFARYYYEGEIQRAESDYG
                     AALDSLDKTRAQARGELNRTASDLQAAAERLARYDGSLLAEARKASDSAEYAYKNGAI
                     GVMDLLDARRTLRAI"
     gene            1293..2336
                     /gene="tsaD"
                     /locus_tag="VI319_11560"
     CDS             1293..2336
                     /gene="tsaD"
                     /locus_tag="VI319_11560"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY6864525.1"
                     /translation="MLVLGIETSCDETGVAVYSSRRGLLAHALHSQVALHDAYGGVVP
                     ELASRDHVRRLLPLVRKVMAQAGVTLGELDAVAYTQGPGLAGALLVGASAACALARGL
                     GVPAVPVHHLEAHLLSPLLAAEAPELPFLALLVSGGHTQLMRVGAIGRYELLGETRDD
                     AAGEAFDKTAKLLGLPYPGGPALAALAARGRPGRVRFPRPMLDRPDLDFSFSGLKTAV
                     ALAHAERAPDGRMSDEARADIAAEFQEAVVDVLVGKCVAAIERSGLDRLVVAGGVGAN
                     ARLRERLSAESARSGFRVFYPRIEFCTDNGAMIALAAALRLEAGLAPVSPGYGFTVRP
                     RWELSEPGGYAAS"
     gene            complement(2321..2974)
                     /gene="plsY"
                     /locus_tag="VI319_11565"
     CDS             complement(2321..2974)
                     /gene="plsY"
                     /locus_tag="VI319_11565"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY6864526.1"
                     /translation="MVASASVVIAYLIGSLSFAVIVSRAFGLPDPHTYGSGNPGATNV
                     LRSGRKIAALLTLVGDAGKGWLAVWLALRVLPEYGADQRWAAACAVAVFVGHLFPVFH
                     GFRGGKGVSTALGTLLAIDPWLGLGTLATWIVIAFFFRYSSLAALVSAAFAPFYAWLI
                     SLVHPRLETLMLAVLPAVILISALLVWRHKSNILKLVAGTESRIGEKRDAGSAQDAA"
     gene            3001..3354
                     /locus_tag="VI319_11570"
     CDS             3001..3354
                     /locus_tag="VI319_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY6864527.1"
                     /translation="MDTIFVRELKLEAWIGLYRHEKIAPQTIELDLEIALPGDAVFAS
                     GRVQDTIDYGVAVERIRALLAAERFGLVESLAERIARLLIDDFGSPRVKVSIAKLGIL
                     RDAKRVGVSIERTRG"
     gene            complement(3370..4302)
                     /gene="xerD"
                     /locus_tag="VI319_11575"
     CDS             complement(3370..4302)
                     /gene="xerD"
                     /locus_tag="VI319_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY6864528.1"
                     /translation="MRAEDAALIDEFCDAVWLQDGLSRNTLDAYRRDLRLLSSWLAER
                     RGPALLALTEADLGRYFADRYAAGRIQPAPHGRKGFKSSTQARLHSSLKRFYQFLARD
                     GRIRADPTLKLDAPKKPQRFPKTLTETDVEALLGEPDAAQPLGLRDRAMLEVLYASGL
                     RVSELVRLKLSEVSTDQGVVRVFGKGAKERLVPLGEEALAWLARWVAEGRPALLKRRA
                     SEFVFVTGRGAPMTRQAFWHLLKKRAARAIPGKSLSPHTLRHAFATHLLNHGADLRVV
                     QLLLGHADISTTQIYTHVARERLKALHAKHHPRA"
     gene            complement(4308..4766)
                     /locus_tag="VI319_11580"
     CDS             complement(4308..4766)
                     /locus_tag="VI319_11580"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY6864529.1"
                     /translation="MPTPFGVVGVRTAGDAVAEIAYLPRSAGALAPRNALAERACRQI
                     ERYLEDPRFRWDLPLAPAGSEFRRRVWRGISAIPAGETRSYGELARSVGSAPRAVGQA
                     CGDNPFPLVVPCHRVVAADGLGGFAHSGARFELSVKRWLLAHEGAPIQHA"
BASE COUNT          688 a         1735 c         1801 g          670 t
ORIGIN      
        1 gatcgcccgc agcgtgcggc gcgcgtcgag caggtccatc acgccgatcg cgccgttctt
       61 gtaagcgtac tctgcggagt ccgaggcctt gcgcgcctcg gccagcagcg acccgtcgta
      121 gcgcgcgagc cgctcggccg ccgcctgcag gtcggacgcc gtgcggttca gctcgccccg
      181 cgcctgcgcc cgcgtcttgt ccagcgagtc gagcgccgcg ccgtagtccg actcggcgcg
      241 ctggatctcg ccttcgtagt agtagcgcgc gaacagcggc accgacacga acaacccgta
      301 ggtattgccg gtgcccgccc cgctgccctg ggcgaagccc gggttcacgg ggtagtggtc
      361 gtacgagagc ccgacggtga cgtcgcgggt gcgcaggctg cgcgcgagat cgcgcgcccc
      421 cgcggccgct tcgacgcggc tgcgcgcggc gcgcacgtcg gggcgccgat cgatcatctc
      481 gtcggtgacc tgagggagat cgaccgaatc gggcgcaggc caggggtcgg tcgcacgcag
      541 gcccgcggcc tgcgcttcgg cgccgatcag gtaggcgagc gcgagctgcg cgcggcgccg
      601 gtcggcttcc gcggagcgcg cgtcgttctg cgcgcgcagc gcgtccaccc gcaggcgcga
      661 cacgtccgcc gccgcgatgt cgcccgcctt gtagcgcagc tcggcggcct cgatggtgcg
      721 ctcgaacagc gcgatgttgt cgcgcgagat cgccgacttg tcctgcgcga acgcgagatc
      781 gtagtacgcc gagcgcagcg cgagcagctg ctggcgcagc acgtcggcgt agtcctgcga
      841 cgcggcggcc tcggagcgct gcgcctgcgc gatgcgcagc tcgcgcttgt cgccgcgctc
      901 gatgagctgg tcgacccgga ccgaggagtc gaccgtcttg tcgcgatagg gaccgccgcc
      961 gacgccgacc tggggattga tctgcgacac gcccaggctc aggttgggat tgggacgcgc
     1021 gcccgccgag acggtgctcg cctgcgcgcc ctcgagcgcg cgccgcgcga gccgcacgtc
     1081 gcggttccct gtggccagct gctgctcggc gtcgcgcaag gtgagtccta tgacgttgtc
     1141 ctgcgccggg gcggcgacgc aggcggcgag catcgcgagt cccgccaccc agcgccgcac
     1201 gcgcttgcta aagttgcgca tgagggccgg cattctatgc agcagcggct gacaaggaac
     1261 tgacatggat cacggaaaag cttattaaaa tcatgctggt tctagggatc gaaacgtcct
     1321 gcgacgaaac gggtgtcgca gtgtacagca gccggcgcgg gctgctcgcg cacgcgctgc
     1381 actctcaggt ggcactgcac gacgcctacg gcggggtcgt tcccgagctc gcgtcgcgcg
     1441 accacgtccg gcgcctgctg ccgctcgtac gcaaggtgat ggcccaggcg ggcgtgacgc
     1501 tcggcgagct cgacgcggtc gcgtatacgc aaggccccgg cctcgcgggc gcgctgctcg
     1561 tgggcgcctc ggccgcgtgc gcgctcgcgc gcgggctcgg cgtcccggcg gtgccggtcc
     1621 atcacctgga ggcgcacctg ctctcgccgc tgctcgcggc cgaggcgccc gagctgccgt
     1681 tcctcgccct gctggtctcg ggcggccaca cccagctgat gcgcgtcggc gccatcggcc
     1741 gctacgagct gctcggcgag acgcgcgacg atgccgccgg cgaggccttc gacaagaccg
     1801 cgaagctgct cgggctgccg tatcccggag gaccggcgct cgccgcgctc gcggcgcgcg
     1861 ggcggcccgg gcgggtgcgc ttcccgcggc cgatgctcga ccggcccgac ctcgatttca
     1921 gcttcagcgg actgaagacc gcggtcgcgc tcgcgcacgc ggagcgcgcg cccgacgggc
     1981 ggatgtccga cgaggcgcgc gccgacatcg cggcagagtt ccaggaagcg gtggtcgacg
     2041 tcctcgtcgg caagtgtgtc gcggcgatcg agcgctctgg gctcgatcgc ctggtggttg
     2101 cgggcggcgt cggcgccaac gcgcgcctgc gcgagcggct gagcgcggag agcgcgcgct
     2161 cgggctttcg cgtcttctac ccgaggatcg agttctgcac cgacaacggc gcgatgatcg
     2221 cgctcgccgc cgcgctgcgc ctggaggccg ggctcgcccc ggtctcgccc ggctacggct
     2281 tcaccgttcg gccgcgctgg gagctctcgg agccgggcgg ctacgcggcg tcctgagccg
     2341 agcccgcgtc gcgcttctcg ccgatccggc tctcggtacc ggcgacgagc ttcaggatgt
     2401 tcgacttgtg ccgccagacg agcagcgccg agatcaggat caccgccggc agcaccgcga
     2461 gcatcagggt ctcgagacgc ggatgcacga gcgagatcag ccaggcgtag aacggcgcga
     2521 acgcggccga caccagcgcc gcgagcgaag aatagcggaa gaagaacgcg atcacgatcc
     2581 aggtggcgag cgtcccgagc ccgagccacg gatcgatcgc gagcagggtg ccgagcgcgg
     2641 tcgagactcc cttgccgcca cggaagccgt gaaacacggg aaacaggtgt ccgacgaaca
     2701 cggcgaccgc gcacgccgcc gcccagcgct ggtccgcgcc gtactcgggc agcacgcgca
     2761 gcgccagcca gaccgcgagc cagcccttgc ccgcgtcgcc gacgagcgtg agcagcgccg
     2821 cgatcttgcg gccgctgcgc agcacgttgg tcgcgccggg gttgcccgag ccgtacgtgt
     2881 gcgggtccgg caggccgaag gcgcgcgaga cgatgaccgc gaaggagagc gagccgatca
     2941 ggtaggcgat cacgacggac gcgctggcga ccatcgttct agaatacctg cattccgccc
     3001 atggacacca tattcgttcg cgagctcaag ctcgaggcgt ggatcggcct gtaccggcac
     3061 gagaagatcg cgccgcagac gatcgagctg gacctcgaga tcgcgctgcc cggcgacgcg
     3121 gtgttcgcga gcggccgcgt gcaggacacg atcgactacg gcgtcgcggt cgagcgcatc
     3181 cgcgcgctgc tcgcggccga gcgcttcggc ctggtcgaga gcctggccga gcggatcgca
     3241 cggctgctga tcgacgactt cggctcgccg cgcgtgaagg tgagcatcgc caagctcggc
     3301 atcctgcgcg acgcgaagcg cgtcggggtc agcatcgagc gcacgcgcgg ttgacgagag
     3361 cgccggcgct caggcgcgcg gatgatgctt cgcgtgcagc gccttcaggc gctcgcgcgc
     3421 gacgtgcgtg tagatctggg tcgtcgagat gtcggcgtgg ccgagcagca gctgcaccac
     3481 gcgcaggtcg gcgccgtggt tgagcaggtg ggtcgcgaac gcgtggcgca gcgtgtgcgg
     3541 cgacagcgac ttgccgggga tggcgcgcgc ggcccgcttc ttgagcaggt gccagaacgc
     3601 ctggcgcgtc atgggggcgc cgcgcccggt gacgaacacg aactcgctcg cgcgccgctt
     3661 gagcagcgcg ggacggcctt cggcgaccca gcgcgcgagc cacgcgagcg cttcctcgcc
     3721 gagcgggacc aggcgctcct tcgcgccctt gccgaaaacg cgcaccacgc cctggtcggt
     3781 gctgacctcg gagagcttca gccgcacgag ctccgacacg cgcaggccgc tcgcgtacag
     3841 cacctcgagc atcgcgcggt cgcgcaggcc gagcggctgc gccgcgtcgg gctcgcccag
     3901 gagcgcctcg acgtcggtct cggtcagcgt cttcgggaag cgctgcggct tcttcggcgc
     3961 gtcgagcttg agcgtcgggt cggcgcggat gcgcccgtcg cgcgcgagaa actggtagaa
     4021 gcgcttcagg ctcgagtgca gcctcgcctg cgtgctcgat ttgaagccct tgcggccgtg
     4081 cggcgcaggt tggatccggc ccgcggcgta gcggtccgcg aaatagcggc cgaggtcggc
     4141 ttcggtcagc gcgagcagcg ccggcccccg gcgctcagcg agccagctgg aaagcagccg
     4201 cagatcgcgc cggtacgcgt ccagggtgtt gcgcgagagg ccgtcctgca gccacaccgc
     4261 gtcgcagaac tcgtcgatga gggcggcgtc ctcggcgcgc atctcagtca cgcatgttgg
     4321 atcggcgccc cctcgtgcgc gagcagccag cgcttgaccg agagctcgaa gcgcgcgccg
     4381 ctgtgcgcga atccgcccag gccatccgcc gccaccacgc ggtggcacgg gacgacgagc
     4441 gggaagggat tgtcgccgca cgcctggccg acggcgcgcg gggcggagcc gacgctgcgc
     4501 gcgagctcgc cgtagctgcg cgtctcgccc gcgggaatcg ccgagatgcc gcgccagacc
     4561 cggcggcgaa actcggaccc tgccggggcg agcgggagat cccagcgaaa ccggggatcc
     4621 tccaggtagc gctcgatctg gcggcaggcg cgctcggcga gcgcgttacg cggcgcgagc
     4681 gcccctgccg agcgcggcag gtacgcgatc tcggcgaccg catcgccggc ggtgcgtacg
     4741 ccgaccaccc cgaagggcgt cggcatgcgc gcctggaaat tgtcgcgatc gctcacgcga
     4801 gcgattctac gcttcaggcg ctcgccggcc gcgcctcgcg tctcatgagc tgcgccgcga
     4861 tggcgaccag gatgagcacc gcgccgacca tgtc
//
LOCUS       DATDMW010000268        32870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_952020, whole genome shotgun sequence.
ACCESSION   DATDMW010000268 DATDMW010000000
VERSION     DATDMW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 32870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32870
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952020"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1545
                     /gene="dnaA"
                     /locus_tag="VI319_11585"
     CDS             202..1545
                     /gene="dnaA"
                     /locus_tag="VI319_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY6864530.1"
                     /translation="MQGFWLSCLSRFEKELPAQQFNTWIRPLKVDATGASSGELRLLA
                     PNRFVLQWVKERFLPRIEAMSAEFYSAPIRVALLLDASPTAAPEPAKAAAPVRAGKAP
                     RPANVERSSLIPEFDFENFVTGKANQLARAAALQVAEHPGTSYNPLFIYGGVGLGKTH
                     LIHAVGNMVLAQQPNARVRYLHAAQYISDVVRAYQQKSFDEFKRYYHSLDLLLIDDIQ
                     FFTGKDRTQEEFFYAFNALIESHKQLIITCDTYPKDLAGIDERLKSRFSWGLTVAIEP
                     PEQEMRVAIILKKAEAISLKLSEEVAFFIAKHIRSNVRELEGALKSVIAYSRFNGRDI
                     TLDTVKEALKDLLSSQNRQITIDNIQKTVAEYYKIKLSDMHSKKRSRSVARPRQLAMA
                     LAKDLTQMSLPDIGDAFGNRDHTTVLHACRTIASLREKNHDINRDYHILEQVLKG"
     gene            1745..2854
                     /gene="dnaN"
                     /locus_tag="VI319_11590"
     CDS             1745..2854
                     /gene="dnaN"
                     /locus_tag="VI319_11590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY6864531.1"
                     /translation="MNLLKVKREALLEPLQAVTGIIERRHTLPILSNVLLSRTDTHID
                     FVATDIEIEITAGMTVEGPGEAKTTTVGARKLLDILRALPEGSEVTLTLADKRLQVKS
                     GKSRFNLQTLPAEDFPRLAAAEGTPTRFSVPQKLLKSLLALVQYAMAQQDIRYYLNGL
                     LMVVDDGQLRLVATDGHRLAFASRDAGSKSLPKQEVILPRKTIIELSKLLADTDDPVA
                     IELSATQAKFSFGRVVLVSKLVDGKFPDYTRVIPQNQPKKLKLDRVALLQSLQRAAIL
                     TSDKFRGVRWVLGDGSLKISCSNTEQEEAQEELDVPYKGDSLDIGFNVGYLLDVLNNL
                     DVSEIECGLGDANSSALFMVPGRSDFKYVVMPMRI"
     gene            2872..5295
                     /gene="gyrB"
                     /locus_tag="VI319_11595"
     CDS             2872..5295
                     /gene="gyrB"
                     /locus_tag="VI319_11595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY6864532.1"
                     /translation="MTDSAKSQQPGDQYDSDSIKVLKGLEAVRKRPGMYIGDTSDGTG
                     LHHMVFEVVDNAIDEALAGFCDTITVTIHTDNSISVIDNGRGIPTEIHKEDEQGRSAA
                     EIVMTELHAGGKFDNNSYKISGGLHGVGVSVVNALSEWLRLTIRRDGLAHFMEFRHGV
                     PVEPLKVLGKTERRGTEVHFLASTETFGTVEYHFDILARRLRELSFLNNGVKISLIDQ
                     RTGKEENFAHTGGVRGFVEFINRSKNVLHQNPFYAIAQKDGITVEVAMQWNDSYQEQV
                     LCFTNNIPQRDGGTHLTGLRAAMTRSLNQYIESNEIAKKAKVETSGDDMREGLSCVLS
                     VKVPEPKFSSQTKDKLVSSEVGPVVQEIVADRLKSFLLERPNDARIICTKIVDAARAR
                     EAARKAREMTRRKGVLDSFGLSAKLADCQEKDPRLCELYIVEGDSAGGSAKQGRDRKF
                     QAILPLKGKILNVEKARFDKLISSQEIVTLIQTLGTGIGKDEYDLSKLRYHRIIVMTD
                     ADVDGAHIRTLLLTFFYRQMPELVEHGHIYIAQPPLYKVKVGKQERYFKDDHERAQFM
                     LAQALVGASLVPSTDAQPIQGQTLEQLAKAYLASEAIVARLSKLIDVEALRALTRGAK
                     VDLSSAEAAQTSARRLKEAIAMNGNGVNVLARYDDKNERHQLVIERSHHGNIRTTVLD
                     DDFVLSGDYQHLERLSQLLQGLVGRDAYVQREDKRQPVADFPQAMGWLLGEVERGASI
                     QRYKGLGEMNPEQLWETTMDPASRRLLKVQIEDGIAADEIFTKLMGDDVEPRRIFIED
                     NALGARLDV"
     gene            complement(5302..7908)
                     /locus_tag="VI319_11600"
     CDS             complement(5302..7908)
                     /locus_tag="VI319_11600"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEY6864533.1"
                     /translation="MSKKLIIAEKPSVANDIARVLGGFTRHGDYYESTGYVLSSAIGH
                     LLELVPPEGVEVKRGKWSFANLPVIPPHFDLQPIERTEDRLKLLVKLLKRKDVTGIVN
                     ACDAGREGELIFRYIMRHAGVKKPVERLWLQSMTPSAISEGFEKLRRDRDMLPLADAA
                     VSRSESDWLVGINGTRAMTAFNSKEGGFYKTTVGRVQTPTLAILVDREEKIRKFVSRD
                     YWEVHATFAAAAGEYAGRWFDESFEKKKDEDPEARAERLWDEQRAQAIRAKCDGRPGV
                     VSEEAKPSTQLSPLLYDLTSLQREANGRFGFSARTTLSLAQALYERHKALTYPRTDSR
                     ALPEDYVGTVKKTLEMFAGRKSGTYAKHAGTILKKSWVKPNRRIFDDKKISDHFAIIP
                     TLQQPKQLNEAETKIYDLVVKRFLAVFFPAAEYLVTTRITRVDGEPFKTEGRVLTQPG
                     WLQVYGKDVDAEDSTRLVPVKDGEKVQTSAVEVRPVQTRPPARFTEATLLSAMEGAGK
                     LVEDEELREAMSEKGLGTPATRAQIIEGLIQEEYVHRNGRELQPTPKAFSLLFALRHF
                     GVTELSSPELTGNWEFKLSQMEHGELERSEFMDHIVDLTKEMVARIKNGEIPDTAFLT
                     LEAPCPKCGGKIQENYRKFQCQKCDFSIWKVVSSREFAPEEIEDLVSKGSVGPLQGFR
                     SRLGKPFAAVVKLSPEFKAEFDFGQAEAQEKVDFSGQTPLGTCPKCGGRVFEQPMAYV
                     CENSVGPERRCDFRSGRIILQRPIEREQLEKLLATGKTDLLHRFISRKGRPFSAFLVR
                     GPDGNVRFEFAPRAGKAPKPEALAAKPAATAKTEATAAKPTRAKAAKPSAAKQAKRRS
                     ARGRTVKAKRAA"
     gene            complement(8113..8571)
                     /locus_tag="VI319_11605"
     CDS             complement(8113..8571)
                     /locus_tag="VI319_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEY6864534.1"
                     /translation="MFDVLVYLFENFYQAEGRPDHETLARKLHAAGFENEDIHDALDW
                     LNGLATPHETGRLECLDLRPTFRGYNAQETAKLSTETRGFLAFLESARILAPRMRELI
                     IERAMALPDEVVGLDKFKVIILMVLWTQRGSVDALVLDELLPDGEKRQMH"
     gene            complement(8593..9660)
                     /gene="dprA"
                     /locus_tag="VI319_11610"
     CDS             complement(8593..9660)
                     /gene="dprA"
                     /locus_tag="VI319_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY6864535.1"
                     /translation="MDSELAAWIDLSLVPGLGGQTIRTLLAAFGLPRHVLEASATTLS
                     RTVPDPIARAIVSRDRAAAVERALEWCSGPSRTVITLADACYPQQLLEIPDPPLLLYL
                     QGDPKYLSASSVAVVGSRNATPQGLANAQAFARALSTSGVAVVSGLALGIDSAAHRGG
                     LEGPAGTVAVLGTGADIVYPKKNQPLADQIASHGALVSEFPLGTPPLQANFPRRNRLI
                     AGLTRGCLVVEAALPSGSMITARLAAEQGRDVFAIPGSIHSPLSKGCHALIKQGAKLV
                     ESAQDVLDELGVAARATPEARAPVPQSKLLGHMGFDPCDIDTLVARSGLTADAVSAML
                     LQLELEGKVGSLSGGLYQRVS"
     gene            complement(9702..10841)
                     /locus_tag="VI319_11615"
     CDS             complement(9702..10841)
                     /locus_tag="VI319_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY6864536.1"
                     /translation="MGRRRPFPDEAVMKKSITAVLASFLCAAALVYAQGDIQLKENAP
                     DSYVVQKGDTLWSIAVKFLKDPWRWPEIWRLNQEQIRNPHEIVPGSTIVLDRTGAGPG
                     GDGQPQLRLGDAQPQQRLQLGQGGSTERLGPKIYSEPISAEAIPAIPANVIEPFLTQP
                     LVIENGGLAKAPRIIATQESRVYLGRGGIAYVTGFGKSQEEIWQIYRQGRPLVDPDDN
                     RTLGYEAVYLGTARVTRGGDPATVQIVSSKQEITAGDRLVAAGKPAPIKYAPHAPTKQ
                     LKGRVIGLYDGLATAEGGRHSIISINKGARDGLEHGHVLALYRRGALIADPESGLSRD
                     NAPKFQLPDERYGLAFVFRVFDNVSYALVMESSRQLTPGDVVQTP"
     gene            10917..11420
                     /gene="def"
                     /locus_tag="VI319_11620"
     CDS             10917..11420
                     /gene="def"
                     /locus_tag="VI319_11620"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY6864537.1"
                     /translation="MASLSILRYPDPRLYKKAATVTEVNEEIRTLVDDMAETMYSAPG
                     IGLAATQVDVHKRVIVIDTSETKDKLLVLINPEIVAREGVQFCEEGCLSLPGIYEPVE
                     RSERVTVRALDREGKAFTLEADKLLAICIQHEMDHLEGKVFVDYLSRLKQQRIKAKLQ
                     KQLRESA"
     gene            11463..12401
                     /gene="fmt"
                     /locus_tag="VI319_11625"
     CDS             11463..12401
                     /gene="fmt"
                     /locus_tag="VI319_11625"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY6864538.1"
                     /translation="MNLIFAGTPSFAARILDALLDSRHRVIAVLTQPDRPAGRGLGAT
                     ASEVKKRALDRGIAVLQPASLHDTASSPELVALRPDIMVVAAYGLILPERVLGIPRLG
                     CINVHASLLPRWRGAAPIQRALLAGDTRTGISIMQMDPGLDTGPVLLQEACAIGDEDT
                     AGTLHDRLATLGAVMCARALDGLQAGALVGSAQSQAEATYAPKIRKEEARIDWRDSAI
                     SVRRRVRAFDPTPGATAVLRGMELKLWSCALGAGDGAPGEVLNASTAGLLVACGEGAV
                     LVTELQRPGGRRLQAGDFLRGCPVSAGERFSVGSAG"
     gene            12466..13764
                     /gene="rsmB"
                     /locus_tag="VI319_11630"
     CDS             12466..13764
                     /gene="rsmB"
                     /locus_tag="VI319_11630"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY6864539.1"
                     /translation="MAEIAFKQSHSLAQSLALAGEVVGRVLNGSSLNAVLANVRTRPI
                     PGPLVAAAQDLSYGALRAYGLVDAALDRLLDKPLADATLRGLLLAALSELMQRPEAAH
                     AVVHQAVEAAGHVERARAKGLVNAVLRNYQRRAAALNSEIESTDAGRFRHPQWWIDLV
                     RSAYPADWRAILQAGNEHPPMTLRVNRRRSTVEAYLAKLRDAGIAARHLGGDAVRLEA
                     PRGVESLPGFASGEASVQDAGAQWAARLLDVAPGMRVLDACAAPGGKTAHILERVDCD
                     LIAADFDRARTDRIAQNLARLGLTARVVVADCREPERFFGAQRFDRILLDAPCSASGV
                     VRRHPDSKWLRRREDIPAFGKTQAAMLDAAWRVLAPGGKLLYATCSVFPAENAEQVAA
                     FLERSPDAAVAPEVPGRPMGQIVPNADSDGFFYALLEKRL"
     gene            13761..14330
                     /locus_tag="VI319_11635"
     CDS             13761..14330
                     /locus_tag="VI319_11635"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEY6864540.1"
                     /translation="MTRSLRCLLLCATLFLGASAVRADPIEVLGVGVEPIDEGYVVHA
                     DFWFELSPRLEEALANGVSLNFMVEFELVRPRWYWFDETAAAEKLQVKLSYLPLSQQY
                     TVASGPVQQSFTSLAEALRVLGRIRSWPVMGRDRISPGQSYVGAIRMRLDTSQLPKAV
                     QVMAMTTRDWTLVSDWRRFRFVAPEKESR"
     gene            14327..16435
                     /locus_tag="VI319_11640"
     CDS             14327..16435
                     /locus_tag="VI319_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6864541.1"
                     /translation="MTYMLIASAAVGGILLFLLATATANSPIFSEYYPLLLWLNAAVA
                     LALLGLVIYQLVRLARQRRAKVFGSLLTFRVLVMFALVGIVPGLLVYTVSLQFLARSI
                     ESWFDVRVEKALDGGLNLGRAALDVMLSDLVLKAQVMALDLSDVPPAQQPVALSRLRE
                     QLGTENAVLMTSGGTVLASTSTPTDRLAPGVPESALRQAHLNRGYGAVEPLGEKGLVL
                     RVIVPLNTLALVDEARFLQLTHEVPQSIADYGESVQTVYRAYRELQLSREGLKTIYIL
                     TLTLTLLLALFSAIALAFLLSRRLSRPLAILAEGTQAVARGDFSRRAPVTSRDELGIL
                     TQSFNSMTEQLGEARNTAQRNQAQLEMANAYLESILANLSAGVMVFDSGFALRIANAG
                     AARILREDLGPLDRVPLSQWRKLEAFAGMLQAQFAMHPAAAWQVQFELLQRVAVLLVR
                     GSLLPGASGGGYVVVFDDITQLISAQRAAAWGEVARRLAHEIKNPLTPIQLSAERLQA
                     KLSRKLDTDDATALHRATETIVAQVTALKGMVDDFRDYARTPIPRLEGLDLNRLVADV
                     LSLYEQSGTLVRARLQPDLPSVRGDPDQLRQVIHNLLRNSQDALAGTSNPRIEVETAA
                     GPGTPGGQAWLKIVDNGCGFSSEIIERAFEPYVTTKPKGSGLGLAIVKKIIDEHQGTI
                     SIENRRPRGAAVLIALPLAA"
     gene            16454..17248
                     /locus_tag="VI319_11645"
                     /pseudo
     CDS             16454..17248
                     /locus_tag="VI319_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            17516..17698
                     /locus_tag="VI319_11650"
                     /pseudo
     CDS             17516..17698
                     /locus_tag="VI319_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524925.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            17718..19091
                     /gene="trkA"
                     /locus_tag="VI319_11655"
     CDS             17718..19091
                     /gene="trkA"
                     /locus_tag="VI319_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEY6864542.1"
                     /translation="MKIVILGAGQVGSSVAESLVSEANDITVVDIDSARLRNLQDRLD
                     LRTVLGSAAHPSVLVEAGIEDCDLVIAVTQSDETNLVACKLAEQLFNVPTRIARIRAT
                     DYLVNERVLGEECFAVNLAICPEQVLTDYIVKLVQFPEALQVLEFAHGKVSLVAVRAF
                     QGGPLVGHQLKDIRSHLPSVNTRIAAIFRGDSPIVPEGDTVVDAGDEVFCLAATRDIR
                     HVMRELRRMDKPVQRMMIAGGGNIGLRLAGALERDYSVKIIEYSKKRCELLSSRLNRA
                     LVLQGDVTDEALLEAENVDDMDMFIAVTNDDENNIMSALLAKRMGAKRVVALINRRSY
                     VDLLQSGQIDIAISPAQATIGTLLAHVRRGHVTQVHSLRRGAAEALEAVVHGDRESCR
                     CIGRRIDEMDLPKGATIAAIVRGDEVIMAHHDTIIQAEDHVIIFVTDKKTLPRVEKLF
                     QVGVGFV"
     gene            19099..20556
                     /locus_tag="VI319_11660"
     CDS             19099..20556
                     /locus_tag="VI319_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEY6864543.1"
                     /translation="MIRLLPVLSVLSVTIAGFAVAMLVPLGISILYADGAQGAYDEAV
                     VATFLAGIALWVLTRKHRTELQARDGFLLMALVWTVLPAFATIPLLVYIKGLAFTDAY
                     FETVAGLTTTGATILSGLDQLPPSINFWRCELQWLGGMGIIVLAVAILPLLGVGGSQI
                     MRAETPGPMKETKLTPRITETAKGLWVVYAGLTVLCMLVMKFAGMSWFDAVLHAFSTV
                     SLGGFSSHDENFSFFRSPGIEAVTIFFMAIAGMNFGTHFLAFRRRSAGPYRRDPEAGA
                     YLAVLGLSVLGIAYFLFAHEVYPSFWTALRYSAFNVVSIATTAGYASTDFYQWPIFAP
                     VWMLFLCCFTTCSGSTGSGIKLIRAELMVRQALREMVRIVHPRAYVPVKLGGATIENN
                     IIFAVLAFMLIWGGSVIAMTMLLTASGVDIITAFSAVVVCICNTGPGLNQVGPGATYA
                     ALGDFPTWILTFAMLLGRLELFTLFIVLTPAFWRK"
     gene            complement(20564..21052)
                     /locus_tag="VI319_11665"
     CDS             complement(20564..21052)
                     /locus_tag="VI319_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864544.1"
                     /translation="MRIALAALALSAAACTPELDWHEFRSDEGAFVAVLPGRPTFEQR
                     ELSGRPGVVMHLWSARAGGALYGVGYANAPKAGRALVASTRDALAANISGRIVADRQI
                     AVGRALGREFEAEGATTTLIARVLVADDKLYQVALIARKERLGAADADTFFSSFRLLP
                     AP"
     gene            complement(21066..24752)
                     /gene="metH"
                     /locus_tag="VI319_11670"
     CDS             complement(21066..24752)
                     /gene="metH"
                     /locus_tag="VI319_11670"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY6864545.1"
                     /translation="MQPDRTHELRALLDERILILDGAMGTEIQRHRLNEADFRGEKFA
                     GFGRDLQGNNDVLTITRPDLIRAIHASYLSAGADIIETNTFNSNAPSQADYGLEEFVG
                     EMNLRAARLAREVAQEFVARTPGRPRFVAGVLGPTNKTASISPDVNDPGFRNIGFDEL
                     VRTYGEAASALVEGGVDLLLLETIFDTLNAKAAIFAIETLFERIGGRLPVIISGTITD
                     QSGRTLTGQTPEAFWNSVRHARPLAVGLNCALGAQLMRPYIEELAKVAETYISCYPNA
                     GLPNPLSETGYDEGPAHTSCLLHEFAESGLVNIVGGCCGTTPEHIAAITAEVRGVKPR
                     VPPRPEHELRLSGLEPLNVGDKSLFVNVGERTNVTGSKAFARMILAGDYAQALTVARQ
                     QVENGAQIIDVNMDEAMLDSEKAMTTFLALIASEPDISRVPVMIDSSKWSVIEAGLKC
                     VQGKCIVNSISLKEGEAEFVRHALLARRYGAAVVVMAFDEHGQADSLQRRIDICRRCY
                     GILIEKAGFAPEDIIFDPNIFAIATGIEEHNNYGLDYIEAVREIRRSLPFVKVSGGVS
                     NVSFSFRGNDPVREAIHTVFLYHAVRAGMTMGIVNAGQLGVYEEIPAELRERVEDVVL
                     NRRPDAGERLVEFATTVKGAARTQAEDLAWRARPVEERLVHALVKGINEWIVEDTEEA
                     RLKCERPIQVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLVPYIEADK
                     LKAGDSRSKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNLGVMVPAQKILDTAKS
                     EGADIIGLSGLITPSLEEMAHVAAEMRRQGFTVPLLIGGATTSRVHTAVKIAPQYSGP
                     TVYVPDASRSVSVCSSLLSEQKAEYARSVQGDYDKIRAQHAAKKGQALVSLDAARANA
                     LRVDWQQYQPTKPKWLGRRVFAGCDLAEIARLIDWSPFFQTWDLAGSYPGILEDPVVG
                     QAARNVLADAQAMLAKIISEKWLTANAVVGLFPAAAVGDDIEIYRDESRADTAMVWHC
                     LRQQAEKAGDRFNLCLADFVAPRDGPVRDYIGAFAVTAGVGADQHVAQFEAKHDDYGA
                     IMLKALADRLAEALAEQMHARVRRELWGYAPDEDLGSDALIAERYRGIRPAPGYPACP
                     DHTEKGPLFALLRAQDAGMTLTESYAMLPAASVSGFYLAHPEARYFAVGKIGEDQAHD
                     YARRKLVKVDETERWLAPVLAYER"
     gene            complement(24968..25672)
                     /locus_tag="VI319_11675"
     CDS             complement(24968..25672)
                     /locus_tag="VI319_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY6864546.1"
                     /translation="MEYAIRTWEIPSLPIVGTNARFPVRRIFCVGRNYVEHQKEMGGD
                     GREQPFFFTKSAHDLVPANANETISVHYPPMTGNYHWETELVAVIGSGGYKITVDKAN
                     DHVWGYAIGLDMTRRDLQQVGKDKGRPWTFGKDFDQAAPAGPITPAARAGHPKKAKLW
                     LKVNGQTRQDSDIDQMIWSIPEQIAFLTQYYTLEAGDLIMTGTPAGVGAAKAGDELVA
                     GIEGLGELRVKILPPR"
     gene            complement(25819..27294)
                     /gene="amt"
                     /locus_tag="VI319_11680"
     CDS             complement(25819..27294)
                     /gene="amt"
                     /locus_tag="VI319_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY6864547.1"
                     /translation="MKKLFAILALLSIAGLCAPTFAQDKPAAVPAPAATATAAAPAAT
                     AAPAPTPNKGDVAWMLTSTALVLMMSVPALALFYGGLVRSKNMLSVLMQVFVVFSLIT
                     VIWCVYGYSLAFTEGNSFFGSLTERLFLKGTFDPIKGDFSMAATFSKNTPLYELVYVA
                     FQATFAAITCCLILGAIVERVRFSAVLIFMVLWFTFSYAPIAHMVWFFPGPDAFTDPK
                     TVDAVTAKAGLIWQWGALDFAGGTVVHINAGVAGLVGAYVVGKRIGFGKEAMTPHSVT
                     MTMIGAALLWMGWFGFNAGSALEANGSAALAFMNTFLATACAVLSWTFGEWIFKGKPS
                     MVGAASGAVAGLVAITPAAGNVGIPGAFVIGIAVGLVCLWGVNGLKKLLGADDSLDVF
                     GVHALGGILGALLTGVFNSPDLGGPGSVQDWVTMKTGYPGIWAQLWIQIKAVGLVFVW
                     TAFVAYVSYKIADLLVGLRVPEEEEREGLDITSHGETAYHM"
     gene            complement(27306..27644)
                     /gene="glnK"
                     /locus_tag="VI319_11685"
     CDS             complement(27306..27644)
                     /gene="glnK"
                     /locus_tag="VI319_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY6864548.1"
                     /translation="MKLVTAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAAIKDSLLDQAIEAIEKSANTGKIGDGKIFVFDLEQVVRI
                     RTGETGAEAL"
     gene            complement(27678..28829)
                     /locus_tag="VI319_11690"
     CDS             complement(27678..28829)
                     /locus_tag="VI319_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6864549.1"
                     /translation="MKKALVATAVSAAFVAPATALAQSAAAVPTLDKVLEASGVTVSG
                     WIDTQYTHANRNLETGFSDRVFDSQNNSFALHQFGLQVAKQPKEGAGGLVNITAGKDA
                     QVIHAFPETAAPGAAATTTSMFDITQAYGQYASGPLTLMAGKFVTLHGTEVIASTGNN
                     NISRSILFGSVPFTHTGLRTVYAMSDTVSLTAGLNNGWDQLQDTTKGKTLELGMSLTP
                     IKPLSIVASSYFGKETTNAIATPGASEGKRSSANLVATYTVSDALSVGAEYLRVQQDN
                     FASLSGGGTIKAKYDGLAGYLTYMITPKLRAAGRLESFKDTDGLRFLANAGTKYREAT
                     ATLGYLPSPSFEVRGEIRQDRASNSVFTESSGAMSKTLLTYALQAIFKF"
     gene            29060..29302
                     /locus_tag="VI319_11695"
     CDS             29060..29302
                     /locus_tag="VI319_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY6864550.1"
                     /translation="MAQANLLDEVGARVREILASSPAKDVETNLRAMLASVFERLQLA
                     TREQLDVQERVLARTREKLSTLEARVAELEARLRQR"
     gene            29305..30807
                     /locus_tag="VI319_11700"
     CDS             29305..30807
                     /locus_tag="VI319_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY6864551.1"
                     /translation="MSLAVLRSRALTGLEAPEVTVEVHLANGLPGFTIVGLPDTEVKE
                     SKDRVRAALLNSRFDFPSRRITVNLAPAELPKESGRFDLPIALGILAASQQLPAEPLA
                     RYEFAGELSLSGEIRPVRGALAMTWRAASKGRAFVLPTASAAEAALVTGAEIYPADSL
                     IAVCRHLRGEVLLGPYPRNAAGATRASYPDLSDVKGQAHAKRALEVAASGGHSVLMAG
                     PPGTGKSMLAARFPGLLPEMTDAEALESAALQSLGSPGFRLERWKQRPFRAPHHSASS
                     VALVGGGGDPRPGEISLAHHGVLFLDELPEFERRVLEALREPLESGRVAISRAARQAE
                     FPASFQLIAAMNPCPCGYLGHYSGRCRCTPDQVARYRRKLSGPLLDRIDVQIEVPSLR
                     EDDLTRLPEGESSLDVRARVQAAYARQLERQGKPNARLAPREIERLCHIGDDAASLLK
                     QAIARINLSARAYHRVLKLARTIADLAGEAGVGITHVAEAIQYRRLDRPA"
     gene            30811..31647
                     /locus_tag="VI319_11705"
     CDS             30811..31647
                     /locus_tag="VI319_11705"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEY6864552.1"
                     /translation="MPTYAIGDVQGCFDELRQLLDAVGFDRRKDRLWLVGDLVNRGPQ
                     SLAVLRFVRELGSAAVTVLGNHDLHLLTYAAGLAKPRPDDTLDEVLAAPDRPELIDWL
                     RGLPMVHVEEDHVLVHAGLLPQWSVQQAEALGREVEAALRGAQHREFLARLYGSKPDR
                     WDDELRDTDRLRVIVNAMTRMRFCTADGVMEFRTKGEAAAAPPGYMPWFDVPGRRSAH
                     HTVICGHWSALGLRVAPTLLALDSGCVWGGRLTAVRLEDRAITQVECRTSAASQSGAR
                     RR"
     gene            complement(31589..32374)
                     /locus_tag="VI319_11710"
     CDS             complement(31589..32374)
                     /locus_tag="VI319_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY6864553.1"
                     /translation="MPANKTPVWLRLARLARLCVHLIRGLLIARFRYPRLSPEQRSDE
                     KRRWSQSLLSILSVAVREKSGTPELPRRCMVALNHISWLDIFVIDAILPATFVAKSEI
                     RSWPVAGWLCTLVGTLYIERGTRAGARAAREAIAGELVDGTLVAICPEGTTTFGDTLE
                     RFHAALFQPAIDAGATIQPVALRYLDASGRRTDAAAYVGETTFLGALWGTVSARRIVV
                     EVTYAPRFGAAGGERRAVAEAAESAIAGALRIARPPRSGTRPE"
     gene            complement(32391..>32870)
                     /locus_tag="VI319_11715"
     CDS             complement(32391..>32870)
                     /locus_tag="VI319_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY6864554.1"
                     /translation="AGELAEHRIPFMFDPGQGLPMFSGKELLRFVDQATYVAVNDYEG
                     KLLEERTGKSLQALAGMVKALILTRGAEGSVIYADGERIDIPCVPAKAVVDPTGCGDA
                     YRAGLLYGIAAGRDWRSTGQLASLMGSLKISERGGQNHWLDRDAIASEYRRHFGRDF"
BASE COUNT         5540 a        10774 c        10782 g         5774 t
ORIGIN      
        1 cgaggggtga aaagtgcgtt tcatgaaacg tcttcctcaa ggcggacaga actttgtatt
       61 agacgatttt tcgctcgctc gtgtcaacgc gcagagccag cggcgtatca gaacagatag
      121 gcgatcgcct gtggataact acatgggaaa cgggtagaat tcagccttgg tttcgcctct
      181 gtccagaaca cagaaaaact catgcaaggt ttctggctct cctgcctgag ccgattcgaa
      241 aaagagcttc ccgctcaaca gtttaatacc tggatccggc ccctgaaagt cgacgcgacc
      301 ggggcctctt cgggggagtt gcgcctgctc gctccgaatc gctttgtgct gcagtgggtg
      361 aaggagcgtt tcctgcctcg gatcgaggcc atgagcgcgg agttctactc cgctccgatc
      421 cgggtcgcgc tcttgctcga cgcgtcgcca acggcggcgc ccgagcccgc caaggccgct
      481 gcgcccgtgc gcgccggcaa ggcgccgcgt cccgcgaacg tcgagcgctc gagcctgatt
      541 ccggagttcg atttcgagaa ttttgtgacc ggtaaggcga accagctcgc gcgcgccgcg
      601 gcgttgcagg tcgcggagca cccgggcacg tcgtacaacc cgctgttcat ctacggcggc
      661 gtgggccttg gcaagacgca cctcattcac gccgtgggca acatggtgct cgcgcaacag
      721 ccgaacgcgc gtgtgcgcta cctccatgcg gcgcagtaca tttcagatgt cgtgcgcgcc
      781 tatcagcaga agtcgttcga cgagttcaag cgctactacc attcgctcga cctgctgctc
      841 atcgacgaca tccagttctt taccggcaag gatcgaacgc aggaagagtt cttctacgct
      901 ttcaacgccc tgatcgaatc gcacaagcag ctcatcatta cctgcgacac ctaccccaag
      961 gacctcgccg gcatcgacga gcgcctgaag tccaggttct cctggggcct gaccgtcgcg
     1021 atcgagccgc ccgagcagga aatgcgggtc gcgatcattc tgaagaaggc cgaggcgatc
     1081 tcgctcaagc tatccgagga ggtggccttc tttatcgcca agcacatccg atccaacgtg
     1141 cgcgagctcg agggcgcctt gaagagcgtg atcgcctact cgcgcttcaa tggtcgagac
     1201 atcacgctcg ataccgtcaa ggaggcgctc aaggacctcc tgtcgtcgca gaaccgccag
     1261 atcacgatcg acaacatcca gaagacggtc gccgagtact acaagatcaa gctctcggac
     1321 atgcattcga agaagcgcag ccgcagcgtg gcccggccgc gccagctggc gatggcgctc
     1381 gccaaggacc tcactcaaat gagcctgccc gatatcggcg acgcgttcgg caaccgcgac
     1441 catacgaccg tactgcacgc ctgccgcacg attgcgagcc ttcgcgagaa aaaccacgac
     1501 attaaccgcg actaccacat tctcgagcag gtcctgaaag gatgaacgga accgggtggc
     1561 agctgggggc gaaatgtggg gaactgctcg gggcggcacg gtccggcggc ttaccccaaa
     1621 cgcgctcaca cggctccggg aacttatcca ccgggtcgat ccccgggaat cccgcgtcgt
     1681 tactggtggg cagggcttat ccacagattc ggcagcgcta atcatcatca tcaaggaata
     1741 ctagatgaac ttactaaaag taaaacgcga agcgctgctc gagccattgc aggccgtgac
     1801 cggaatcatc gagcggcgtc acacgctacc cattctgtcc aacgtgctgc tttcgcgtac
     1861 ggacacgcac atcgacttcg tcgcgaccga catcgagatc gagattactg ccggcatgac
     1921 ggtcgaaggt ccgggagagg cgaagaccac cactgttggc gcgcgcaaac tgttagacat
     1981 cctgcgcgcg cttcccgaag gctccgaggt gacgctcact ctcgccgaca agcggctgca
     2041 agtgaagtcc ggcaagagcc ggttcaacct gcagaccctc ccagcggaag atttcccgcg
     2101 tctggccgcg gccgagggga cgccgacgcg cttctcggtc ccgcagaaac tgcttaagtc
     2161 gctgctggcc ctggtccagt acgcaatggc ccagcaagac atccggtact acttgaacgg
     2221 tctgctcatg gtcgtggacg atggccagct tcggctcgtc gcgacggatg ggcaccgcct
     2281 ggctttcgcg agccgcgatg cgggctccaa gtcgttgccg aagcaggagg tcatcctacc
     2341 caggaagacc atcatcgagc tctcgaagct tctcgctgat accgacgacc cggtcgcgat
     2401 cgagctttcc gcgacccagg caaagttcag cttcgggcgg gtggtattgg tgtcgaagct
     2461 cgtcgacgga aaatttccgg attacacgcg cgtgattccg caaaaccagc ccaagaagct
     2521 gaaactggac cgggtcgccc tgctgcagtc gctgcagcgc gccgccatcc tgacaagcga
     2581 caagttccgc ggggtacggt gggtgttggg cgacggtagc ctgaagatca gctgcagcaa
     2641 caccgagcag gaagaggctc aggaagagtt ggacgtcccg tacaagggcg actcgctcga
     2701 catcgggttc aacgtagggt atctgctgga cgtgctcaac aacctcgacg tctccgagat
     2761 cgaatgcggc ctcggcgacg cgaactcgag cgcgcttttc atggttccag ggcgcagcga
     2821 cttcaagtac gtcgtgatgc cgatgcggat ttgagaggcg tcgggaagaa aatgacggat
     2881 agcgcaaaaa gccagcagcc tggcgaccaa tacgattcgg acagcatcaa agtcctgaag
     2941 ggactggaag ccgtgcgcaa gcggcccgga atgtacatcg gcgatacgtc cgacggcacc
     3001 ggtctgcacc atatggtttt cgaggtggtc gacaacgcca tcgacgaggc actcgccggc
     3061 ttctgcgaca ccatcacggt cacgatccac accgacaact cgatcagcgt catcgacaac
     3121 ggccgcggca tcccgaccga gattcacaag gaggacgagc aggggcgctc ggcagccgag
     3181 atcgtcatga ccgagctgca cgccggcgga aagttcgaca acaactcgta caagatctcg
     3241 ggcggcctgc acggcgtcgg cgtctccgtg gtgaatgcgc tctcggaatg gctgcgcctc
     3301 accattcggc gcgacgggct cgcgcatttc atggagtttc gccatggggt gccggtcgag
     3361 ccgctgaagg ttctcggcaa gaccgagcgc cgcgggaccg aggtgcactt cctggcaagc
     3421 accgagacct tcggcactgt cgagtaccac ttcgacattc tcgcgcggcg cctgcgggag
     3481 ctgtcgttcc tgaacaacgg cgtgaagatc agcctgatcg accaacgcac cgggaaggag
     3541 gaaaacttcg cccataccgg cggcgtgaga ggcttcgtcg agttcatcaa ccgcagcaag
     3601 aatgtcctgc atcagaatcc gttctatgcg atcgcgcaga aggacgggat cacggtcgaa
     3661 gtcgcgatgc agtggaacga ctcgtaccag gagcaggtgc tctgcttcac gaacaacatc
     3721 ccgcagcgcg acggaggtac ccaccttacg ggcttgcgcg cggcgatgac gcgcagcctg
     3781 aaccagtaca tcgagtcaaa cgagatcgcg aaaaaggcga aggtcgagac gagcggtgac
     3841 gacatgcgcg agggcctctc atgcgtgcta tcggtcaagg ttccggagcc gaagttctcg
     3901 tcgcagacca aggacaagct cgtctcttcc gaggtcgggc cggtggtgca agagatcgtc
     3961 gccgatcgcc tgaaatcgtt cctgctcgag cgccccaatg atgcgcggat catctgcacc
     4021 aagatcgtgg atgcggcgcg cgcccgcgag gccgcgcgca aggcgcgcga gatgacccgg
     4081 cgcaagggcg tgctggactc tttcgggctg tccgcgaagc tggccgactg tcaggaaaag
     4141 gatccgcgcc tgtgcgagct ctatatcgtc gagggcgact ctgccggggg ctcggccaag
     4201 cagggacgcg accgcaagtt ccaggcgatc ctgccgctca aaggcaagat cctgaacgtg
     4261 gagaaagcgc gcttcgacaa gctgatctcc tcgcaggaga tcgtgacgct gatccagacg
     4321 ctagggacgg gaatcggcaa ggacgaatac gacctgtcca agctgcgtta ccaccgcatc
     4381 atcgtcatga ccgacgccga cgtcgacgga gcgcacatcc gcacgctgct ccttacgttc
     4441 ttctatcggc agatgcccga gctcgtcgag cacggccata tctatatcgc ccagccgccc
     4501 ctgtacaagg tgaaggtcgg aaagcaggag cgttatttca aggacgatca cgagcgcgcg
     4561 caattcatgc tcgcccaggc gctggtcgga gcatcgctcg tcccgagcac ggatgcccag
     4621 ccgatccaag gccagaccct ggagcagctg gcgaaggcct acctcgcctc cgaagcgatc
     4681 gtggcgcgcc tttccaagct gatcgacgtc gaggcgctgc gggcgctcac ccggggcgcc
     4741 aaggtcgatc tctcgagcgc cgaggcggcg cagacgagcg cgcgccgcct gaaggaggcg
     4801 atcgccatga acggcaatgg cgtgaacgtc ctggcccgat acgacgacaa gaacgagcgc
     4861 caccagctgg tgatcgaacg cagtcatcac ggcaatatcc gcacgacggt actcgacgac
     4921 gacttcgtgc tctccggtga ttaccagcac cttgagcggc tgtcgcagct gctccagggt
     4981 ctcgtcgggc gcgatgctta cgtacagcgc gaggacaagc ggcagcccgt cgccgatttt
     5041 ccgcaggcta tgggctggct gctgggagag gtggagcgcg gtgcgtcgat acaacgctac
     5101 aagggcctcg gcgagatgaa cccggagcag ctctgggaga cgacgatgga tccggcgtcc
     5161 cggcgtctcc tcaaggtcca gatcgaagac ggaatcgccg ctgacgagat cttcaccaag
     5221 ctgatgggcg acgacgtcga gccgcgccgc atcttcatcg aggacaacgc gctcggcgcg
     5281 cggttggacg tctgacgccg ctcaggcggc tctcttcgct ttcacggtcc tgccccgggc
     5341 ggagcggcgc ttggcttgct tcgcggccga cggcttcgct gctttggcac gcgtcggttt
     5401 cgccgccgtc gcttccgtct tggcggtagc ggccggcttc gcggccagcg cctcgggctt
     5461 gggcgccttg ccggcgcggg gagcgaactc gaaccgcaca ttgccgtccg ggccgcgcac
     5521 gagaaatgcc gagaacgggc ggcctttgcg cgatatgaaa cggtgcagca ggtcggtctt
     5581 acccgtcgca agcagcttct cgagctgctc gcgctcgatc gggcgctgca ggatgatacg
     5641 tccggagcgg aaatcgcaac ggcgctcggg gccgacgctg ttctcgcaca cataagccat
     5701 cggctgctcg aacacgcgac ctccgcattt cgggcacgtg ccgagcggcg tttgtcccga
     5761 gaagtcgact ttctcctgcg cttccgcctg gccgaaatcg aactcggcct tgaactccgg
     5821 cgaaagcttc accacggcgg cgaagggctt gccgaggcgg ctgcggaacc cttggagcgg
     5881 ccccaccgag cccttggaca cgaggtcttc gatctcctcg ggggcgaact cgcggctcga
     5941 taccaccttc cagatcgaga agtcgcactt ctggcactgg aacttgcggt agttctcctg
     6001 gatctttccg ccgcatttcg ggcacggcgc ctccagcgtg aggaacgcgg tgtccgggat
     6061 ctcgccgttc ttgatgcgag ccaccatctc cttcgtgaga tcgacgatgt gatccatgaa
     6121 ttcgctgcgc tcgagctcgc cgtgctccat ctgactgagc ttgaactccc agttgccggt
     6181 cagctccggc gacgacaatt cggtgacccc gaaatggcgc agcgcgaaca gcagcgagaa
     6241 cgccttgggc gtgggctgca attcgcgccc gttgcgatgc acgtactcct cctggatgag
     6301 gccttcgatg atttgcgccc gagtcgccgg cgtgccgagc cccttttcgc tcattgcctc
     6361 gcgcagctcc tcgtcttcga cgagcttgcc ggcgccttcc atcgccgaca gcagcgttgc
     6421 ctcggtgaag cgggccggcg gccgcgtctg caccggccgc acttcgacgg ccgacgtctg
     6481 caccttctcg ccgtccttca cggggacgag tcgagtcgag tcctctgcgt cgacgtcctt
     6541 gccgtagact tgcagccagc ccggctgagt caggacacgc ccttccgtct tgaacggctc
     6601 gccgtcgacg cgcgtgatgc gcgtggtcac gaggtattcg gccgccggga agaacaccgc
     6661 gaggaaacgc ttcactacga ggtcgtagat cttggtttcc gcctcgttga gctgcttcgg
     6721 ctgctgcagc gtcgggatga tggcgaaatg atccgaaatc ttcttgtcgt cgaagatccg
     6781 gcggttgggc ttcacccagg attttttcag gatcgtgccg gcgtgcttgg cgtacgtacc
     6841 gctcttgcga ccggcgaaca tctcgagagt cttcttgact gttccgacgt agtcttcggg
     6901 tagcgcgcgc gagtccgtgc gcggatacgt gagcgcctta tggcgctcgt acagcgcttg
     6961 cgcgagcgag agcgtcgtgc gcgccgagaa gccgaagcgc ccgttcgcct cgcgctgcag
     7021 gctggtgagg tcgtagagga gcggcgagag ctgggtcgag ggcttcgcct cctcgctgac
     7081 gacgcccggc ctgccatcgc atttcgcgcg gatcgcctgc gctcgctgct cgtcccacaa
     7141 gcgctcggcc ctggcctcgg gatcctcgtc cttcttcttc tcgaaggact cgtcgaacca
     7201 tcggccggcg tactctcccg cggccgcggc aaaggtcgcg tgcacttccc agtagtcgcg
     7261 cgacacgaac ttgcggatct tctcctcgcg gtcgacgagg atcgcgagcg tgggtgtctg
     7321 cacgcgaccg acggtcgtct tgtagaagcc gccctctttc gaattgaagg ccgtcatcgc
     7381 gcgcgtgccg ttgatgccga ccagccagtc cgattccgac cgggacacgg cggcgtcggc
     7441 gagcggcagc atgtcgcgat cgcgccgcag cttctcgaag ccctcgctga tggcggaagg
     7501 cgtcatcgat tgcagccaca gtcgctcgac cggcttcttc acgccggcgt gccgcatgat
     7561 gtagcggaag atgagctcgc cttcccggcc cgcgtcgcag gcgttgacga taccggttac
     7621 gtccttgcgc ttgagcagct tcacgagcag cttgaggcgg tcctcggtcc gctcgatggg
     7681 ctgcagatcg aaatgcggcg ggatgaccgg tagattggcg aacgaccact tgccgcgctt
     7741 gacttcgacc ccttcgggag gaaccagctc gagcagatgg ccgatcgcgg aagacaggac
     7801 gtagcccgtg ctctcgtagt aatcgccgtg gcgcgtgaac ccccccagaa cccgggcgat
     7861 gtcgttcgcg accgaaggct tttccgcgat gatgagtttc ttgctcatac gatgcgttca
     7921 cggcgaccta cgcaggcatt gaccgtgcga cgcatttcgc gttcacggga tgcacaagag
     7981 ggaagaatgg gtgcgccgat cgaagcgcag catgataagt gcttttcggg ccgcgacgca
     8041 agaaagcggt cggctaacct cgcaaggtcg cgatcgattt cggaaaaacg gcgaccggcg
     8101 cggcgcgccg gatcaatgca tctggcgctt ctcgccgtcc gggagcagct cgtcgagcac
     8161 cagcgcgtcc acgctcccgc gctgcgtcca gaggaccatg aggatgatca ccttgaactt
     8221 gtcgagtccg acgacttcgt cggggagggc catggcgcgc tcgatgatca gctcgcgcat
     8281 ccgcggcgca agaatgcgcg cgctttcgag gaatgccagg aagccgcggg tctcggtgga
     8341 aagcttggcg gtctcctggg cgttgtaccc gcggaaggtc ggacgcaagt cgaggcactc
     8401 gaggcgcccg gtttcgtgcg gggtcgccag gccgttgagc cagtcgaggg cgtcgtggat
     8461 gtcttcgttc tcgaatccgg cggcgtgcag cttgcgtgcc agcgtctcgt ggtcggggcg
     8521 gccttcggct tgatagaagt tttcgaagag atatacgaga acgtcgaaca tttttttgcg
     8581 attgtcctat tctcacgaaa cccgttgata taggcctccg gagaggctcc cgaccttccc
     8641 ttccagttcg agttgcagca gcattgccga tacggcgtcc gcggtcaagc cgctccgcgc
     8701 gaccagcgtg tcgatatcgc acggatcgaa acccatgtgc cccagcagct tgctctgcgg
     8761 cactggagcg cgcgcttcgg gcgtcgcgcg ggccgcgacg ccgagctcgt cgagcacgtc
     8821 ctgcgcgctc tcgaccagct tcgcgccctg cttgatcagc gcgtggcacc ctttggagag
     8881 cggcgaatgt atggatccgg gaatggcgaa tacgtcgcgt ccctgctcgg cggcgagacg
     8941 cgccgtgatc atggaccccg acggcagcgc cgcttcgacg accaggcagc cgcgcgtcaa
     9001 ccccgcgatc aaacggttcc ggcgcgggaa gttggcctgc aagggcggcg tgccgagcgg
     9061 aaactccgaa accagcgcgc catgcgaggc gatctggtcg gcgagtggct ggttcttctt
     9121 cgggtagacg atatcggcgc cggttcccag gaccgctacc gttcccgcgg gtccctcgag
     9181 tccgccgcgg tgtgcggcgc tgtcgattcc cagcgcgaga ccgctcacga ccgccacgcc
     9241 cgacgtgctg agcgcgcgag cgaaagcctg cgcgttcgcg aggccctggg gcgtggcgtt
     9301 gcggctgccg acgaccgcta cggagctcgc cgataggtac ttcgggtcgc cttgcaggta
     9361 cagcagcaga ggcggatccg gtatttcgag cagctgctgc ggataacagg catcggccag
     9421 cgtgatgacc gtcctgctgg ggccggagca ccattcgagc gcgcgctcga ccgcggctgc
     9481 gcgatcacgg ctcacaatag cgcgcgcaat cgggtcgggc acggtgcgcg agagcgtcgt
     9541 ggcggacgcc tcgagcacgt gccgcggcaa tccgaatgcc gcaagcagcg tccgtatggt
     9601 ctgcccgccg agccccggaa cgaggctgag gtcgatccag gcggcgagtt cggagtccaa
     9661 gagatcgccc gcagtgcttc gacgacgctc gggcgcttcg gtcagggcgt ttgcaccacg
     9721 tcgcccggcg tgagctgcct ggagctctcc atcacgagcg catacgagac attgtcgaac
     9781 actctgaata caaacgcgag accgtagcgc tcgtcgggca gctggaactt cggcgcgttg
     9841 tcgcgcgaca atcccgattc gggatcggcg atgagggcgc cgcgccggta cagcgcgagc
     9901 acatgaccgt gctcgaggcc gtcgcgagcg cccttgttga tcgagatgat cgaatgccga
     9961 ccgccctcgg cggtcgccaa gccgtcgtag aggccgatca cgcgcccctt caactgcttg
    10021 gtcggcgcat gcggcgcgta cttgatcggc gccggcttcc ccgccgcgac caagcggtcg
    10081 ccagcggtga tctcctgttt tgacgagacg atctgaacgg tcgccgggtc gccgccgcgc
    10141 gtcacgcgcg cggtcccgag gtagaccgct tcgtagccga gcgttctgtt gtcatcggga
    10201 tcgaccagag gccgcccctg tcggtagatc tgccagatct cctcctgcga cttgccgaat
    10261 ccagtgacgt aggcgattcc gcctcgtcca agatagacac ggctctcctg cgtggcgatg
    10321 atgcgcggcg ctttcgcgag ccccccgttc tcgatcacga gcggctgcgt cagaaacggc
    10381 tcgatgacgt tcgcagggat ggccggaatt gcctccgccg agatgggctc gctgtagatc
    10441 ttcggaccga ggcgttcagt ggaaccgcct tggccgagct ggagccgctg ctgcggctga
    10501 gcatccccga gtcgcaactg cggctgaccg tccccgcccg ggcccgcgcc ggtccggtcg
    10561 aggacgatcg tgctacccgg gacgatttcg tgcgggttgc ggatctgctc ctggttgagg
    10621 cgccatatct cgggccagcg ccacgggtcc ttcagaaact tgacggcgat gctccacagc
    10681 gtgtcgccct tctgcacaac gtagctgtcc ggagcgtttt ccttgagctg aatgtcgcct
    10741 tgcgcataga cgagagcagc ggcgcagagg aaggacgcga gaacggctgt gatagacttt
    10801 ttcatgactg cctcgtctgg aaacgggcgt cgccgcccca acgtgcccca gagtagcccc
    10861 ttttttacag tccgcattaa attctgaacg taatgcgtta aatttgcaag ttttttatgg
    10921 cttccctgag catccttcgt tatccagatc cccgcctcta caaaaaggcg gcgaccgtca
    10981 cagaggtgaa cgaagagata cgcactctgg tggacgacat ggccgagacg atgtactcgg
    11041 cgcccgggat cggcctggcc gcgacgcaag tcgatgtgca caagcgcgtt atcgtcatcg
    11101 acacgtcgga gacgaaggac aagctcctcg tgctgatcaa tcccgagatc gtcgcgcgtg
    11161 aaggcgtaca gttctgcgag gaaggctgcc tttcgctgcc ggggatctac gagccggtgg
    11221 agcgctcgga gcgcgttacg gtgcgcgctc tcgaccgcga gggcaaggct ttcacgctcg
    11281 aagcggacaa gctcctcgcc atctgcatcc agcacgagat ggaccatctc gagggcaagg
    11341 tcttcgtgga ttatctgtcg cgcctcaagc agcagcgcat caaggccaag ttgcagaagc
    11401 agcttcgcga gagcgcctag accgcttcgc gacgcggcgg tcagcccgac ggaggccgga
    11461 tcatgaacct catcttcgcg ggcacgccgt ctttcgcggc tcggatcctc gatgccctcc
    11521 tcgactcgcg gcaccgcgtg atcgccgtcc tgacgcagcc cgaccgcccg gcgggccgcg
    11581 ggctcggcgc cacggcctcc gaggtcaaaa agcgcgcgct cgaccgcgga atcgcggtgc
    11641 tgcagccggc gtcgttgcat gacaccgcgt cgtcgcccga gctcgtcgcc ctgcggcccg
    11701 acatcatggt cgtcgccgcg tacggcctga tccttcccga gcgcgtgctc ggcatcccgc
    11761 gcctggggtg catcaacgtg catgcttcat tgttgcctcg ctggcgcggg gcggcgccga
    11821 tccagcgcgc cttgctcgcg ggcgacaccc ggaccgggat atcgatcatg cagatggacc
    11881 ccgggctcga caccgggccg gtgttgctgc aggaggcctg cgccatcggc gacgaggata
    11941 ccgccggcac cctgcacgat cgcctggcga cgctcggtgc cgtgatgtgc gcgcgcgcgc
    12001 tcgacggctt gcaggctggt gcgctggtcg ggtcggcgca atcgcaggcg gaagcgacct
    12061 atgctccgaa gatccgcaag gaggaggccc gcattgactg gcgcgacagc gcgatatccg
    12121 tgcggcgccg cgtgcgggcg ttcgatccga cgccgggggc cactgcggtg ctgcgcggca
    12181 tggagttgaa gctctggtct tgcgcactgg gagcgggcga cggagcgccc ggcgaagtat
    12241 tgaacgcgtc gaccgcaggt ctcctggtgg cctgcggcga gggcgcggtc ctggtcaccg
    12301 agctgcaacg tcccggtgga cggcgtctcc aagccggcga tttcctgcgc ggttgtcccg
    12361 tgtcggcggg cgaacggttc tccgtcggat ccgcggggtg atcggccgcg cccgcgtgcg
    12421 cgattcacta taatgccgtc tcgctcgtaa ccttgcgccc gcgccttggc cgaaatcgcc
    12481 ttcaaacaat cccattcgct ggcgcagtcc ctggcgcttg ccggcgaagt cgtcgggcgt
    12541 gtgttgaacg gctcgagcct caacgcggtc ctcgcgaatg tcaggacgcg ccccatcccc
    12601 ggcccgctcg ttgccgcggc gcaagacctg tcctacggcg cgctgcgggc gtacgggctc
    12661 gtcgacgcgg cgctcgaccg cctgctcgac aagccgctcg ccgatgctac gctgcgcggt
    12721 ctgcttctcg ccgcgttgtc cgagctcatg cagcgacccg aggccgcgca cgcggtcgtg
    12781 caccaggccg tggaggccgc gggtcacgtc gagcgagcgc gtgccaaagg cttggtgaac
    12841 gcggtgctgc gaaactatca gcgtcgcgcc gccgctctga attcggagat agagtcgacc
    12901 gacgccggca ggtttcgcca cccgcagtgg tggatcgatc tcgtcagatc ggcttacccc
    12961 gcggattggc gtgccatcct gcaggcgggc aacgagcatc ccccgatgac gctgcgcgtg
    13021 aaccgtcgtc gctcgacggt cgaagcctac ctcgcgaagc tgcgcgatgc gggcatcgcc
    13081 gcgcgtcacc tcgggggcga cgccgtgcgt ctcgaggcgc cccggggagt cgaatcgctc
    13141 ccgggatttg cgagcggcga agcatcggtt caggacgccg gagcgcaatg ggcggcacgg
    13201 ctgctcgacg tcgccccggg catgcgcgtg ctcgacgcgt gcgccgcccc gggcgggaag
    13261 accgcgcata tcctcgagcg ggtcgactgc gatctcatcg cggccgactt cgatcgcgct
    13321 cgcaccgaca ggatcgcgca gaaccttgcg cggctcggct tgacggcgcg cgtcgtggtg
    13381 gccgactgcc gtgagccgga gcgtttcttc ggtgctcagc gcttcgaccg catcctgctc
    13441 gatgcgccgt gctcggcctc aggagtggtg cgccggcatc cggacagcaa gtggctgcgc
    13501 cggcgagagg acatcccggc tttcggcaag actcaagccg ccatgctcga tgcggcttgg
    13561 cgggtgctgg ccccgggagg caagctgctc tatgcgacgt gctcggtgtt tccggccgag
    13621 aacgccgagc aggtcgccgc cttcctcgag cgcagcccgg acgccgcggt cgcgccggag
    13681 gtccccggcc gaccgatggg ccagatcgtc ccgaacgccg acagcgacgg cttcttctac
    13741 gcgctgctgg aaaagcggct atgacgcgat ccctgcgctg cctgctgttg tgcgccacgc
    13801 tgttcctcgg cgcaagcgcg gtccgtgccg atcccatcga ggtcctcggc gttggcgtgg
    13861 agcccatcga cgaaggttac gtcgtgcacg cggacttctg gttcgagctg tcgcctcgac
    13921 tcgaggaggc gctcgcgaat ggcgtctcgc tcaatttcat ggtcgaattc gagctggtgc
    13981 gcccccggtg gtactggttc gacgagaccg cggccgcgga gaagctccag gtcaagctct
    14041 catatctgcc cctgtcgcag caatacaccg tggcgagcgg cccggtgcag cagagcttta
    14101 cgtcgctcgc tgaagccctg cgcgtactgg gacgcatccg gagctggccc gtcatgggcc
    14161 gggaccggat ctccccggga caaagctacg tcggcgcgat ccgcatgcgg ctcgacacgt
    14221 cgcagcttcc caaggcggtc caggtgatgg ccatgaccac ccgcgattgg acgctcgtgt
    14281 ctgactggcg acgtttcagg ttcgtcgcac ccgagaagga gtcgcgatga cttacatgct
    14341 gatcgcaagc gcggcggtgg gcggcatcct gctgttcctg ctggcgaccg caacggccaa
    14401 ctcgccgata ttctcggaat actatccgtt gctgctctgg ctgaacgcgg ccgtcgcgct
    14461 cgctctgctc ggccttgtga tctaccagct ggtgcgcctc gcgcgccagc ggcgcgccaa
    14521 ggttttcgga tcgctcctca cctttcgcgt actggtcatg ttcgcgctcg tcggcatcgt
    14581 gccgggcctg ctcgtctata cggtgtcgct gcagttcctc gcgcgcagca tcgagtcgtg
    14641 gttcgacgtg cgcgtcgaga aggcgctcga cggtgggctc aatctcggac gcgcagcgct
    14701 ggatgtcatg ctgagcgacc tcgtcctcaa agcccaggtg atggcgctcg atctttcgga
    14761 tgtcccaccc gcgcagcagc cggtcgcgct gagccgcctg cgcgagcagc tcggaaccga
    14821 gaacgccgtg ctcatgacgt cgggcggaac ggtgctcgcg agcaccagta cgcccaccga
    14881 ccggctggcg cccggcgtcc cggagagcgc gctgcgccag gcccacttga accgcggcta
    14941 cggcgccgtc gagccgctgg gcgagaaagg cctcgtgctg cgcgtcatcg tgccgctcaa
    15001 cacgctcgcg ctcgtcgacg aggcgcgctt ccttcagctc acgcacgagg tgccgcagtc
    15061 gatcgccgac tatggcgaat cggtacagac ggtgtaccgg gcgtaccggg agcttcagct
    15121 ctcgcgcgag ggattgaaga ccatttacat cctgaccctg acgctcaccc tgctgctggc
    15181 gctcttctcg gcgatcgcgc tcgcgttcct cctgtcccgg cgcctgtcgc ggccgctcgc
    15241 catcctcgcc gagggcacgc aggcggtcgc gcggggcgac ttttcgaggc gggcgcccgt
    15301 caccagccgc gacgagctcg gcatcctgac gcagtcgttc aacagcatga ccgagcagct
    15361 cggcgaggct cgcaacacgg cgcagcgcaa ccaggcgcag ctcgagatgg ccaacgcgta
    15421 tctagagagc atcctggcga acctgtccgc tggcgtgatg gtgttcgaca gtggcttcgc
    15481 cttgcgcatc gccaatgcgg gggccgctcg catccttcgc gaggatctgg gtcccctcga
    15541 ccgcgtgccc ctctcccaat ggcgcaagct cgaggctttc gccgggatgc tgcaggcgca
    15601 gttcgccatg catcctgcgg cggcgtggca ggttcagttc gagctgttgc agcgggtcgc
    15661 cgtgctcctg gtccgggggt cgttgctgcc gggcgcgagc ggcggcggct acgtggtggt
    15721 gttcgacgac attacccagc tgatctccgc gcaacgggca gcggcgtggg gcgaagtcgc
    15781 gcgccggctc gcgcacgaga tcaagaatcc gctcaccccg atccagctct ccgccgagcg
    15841 cctgcaagcg aagctctccc gcaagctcga caccgacgac gccacggcgc tccaccgtgc
    15901 gaccgagacc atcgtcgcgc aagtcacagc actcaagggc atggtcgacg atttccgcga
    15961 ctatgcgcgg acaccgattc cccggctcga gggcctcgat ctgaaccggc tggtcgccga
    16021 cgtgctatcg ctgtacgagc aatcgggaac tctggttcgg gcgcgcctgc agcccgacct
    16081 tccgtcggtg cggggcgacc ccgatcagtt gcggcaggtc atacacaacc tcctgcggaa
    16141 ctcgcaggac gctctcgccg ggacttccaa tccgaggatc gaagtcgaga ccgccgccgg
    16201 gccggggacg cccggcgggc aggcgtggct caagatcgtc gacaacggct gcggtttctc
    16261 gagcgaaatc atcgagcggg ccttcgagcc gtatgtcacg acgaaaccca agggctcggg
    16321 gctgggtctc gcgatcgtga agaagatcat cgacgagcat caaggtacga tcagcatcga
    16381 gaatcggcgg ccgcggggag ccgccgttct gatcgctctc ccgctggccg cgtgaggaag
    16441 acggcgcgcc actatggccc agatactggt agttgacgac gagatcggca tccgcgagct
    16501 gttgtccgag attctctccg acgagggaca tacggtacag ctcgccgaga gcgcgacggc
    16561 agcgcgcacc gcgcgggcgc gccagcggcc ggacctcgtg ctgctcgaca tttggatgcc
    16621 cgacgccgac gggatcacgt tgctcaagga atgggcggcc ggcggccagc tcaccatgcc
    16681 ggtcatcatg atgtcgggcc acggcacgat cgaaacggcc gtcgaagcga cgcgaatcgg
    16741 cgcgatggat ttcctggaaa aaccgatcgc gctgcagaag ctgctcggta cggtgaagcg
    16801 ggtgctgcgc ttctccgagg cgaggacgat acggccgctc tcgctcacgg gcgtcgggcg
    16861 ctcgcccgcg ctcgccgatt tgcgcaagcg tctcgcgcag gtcgaggccg tcaacgcgag
    16921 cgtcctgctt cgcggcgaga agggctccat gcccgagctg ttcgcgcgtg acctgcagcc
    16981 gccgaacggc ccatggctgg acgggagcca ggcgctcctc gaaggttcgc aggacgtgct
    17041 gcaacaggcg gccggcgggg tgctgttcgt cgaggagctc acggcccttt cgcgggcgca
    17101 gcagaagcac ctcgcattct tggcgacgcg ctcggaaaag cacagggttc gcttcgtatc
    17161 cttcacttgc gaggagccgc ggcgcctggt ggacgcacac gggttcgatg ccgcgctctt
    17221 gcaggcgctc tcgaacgtca tgatcgcgct gccgccgctg cgggagcacc cggaggacat
    17281 ccccgagatc gcgagcgtca tgctcgctca gctggtcgaa gcacgcttct gccccccgcg
    17341 acatttctcc accgctgcgc tgaatcagct gcgcaatttc gactggcccg gtaatctgca
    17401 ggatctggcg gcggccgtgc gcacgctggc ggccacggcg ctcgacgaag agatcggcac
    17461 aaccgatgtc gaatcggtgg tacctcagtt cgcagcgccg gccaagggca tcgatctccc
    17521 gctcgatctg cccttgcgcg atgcgcgcga ggctttcgag cgcgcgtatt tcgagcacca
    17581 cctggcgcta gagaacgggt cgatcgcccg ggtggcggag aagtgcggtc tggagcgcac
    17641 gcacctctat cggaagctca aggcgctcgg catacacacc ggacgccgcg aagaatgacc
    17701 cgggctcgga tcacaagatg aaaatagtca ttctcggtgc gggccaggtc gggtcctcgg
    17761 tcgcggaaag cctggtttcc gaggcgaacg acatcacggt cgtcgacatc gacagcgcgc
    17821 ggctgcgcaa cctgcaggat cggctcgatc tccgcacggt cctcggcagc gcggcgcatc
    17881 cttcggttct ggtcgaagcg ggcatcgagg attgcgacct ggtgatcgcc gtcacgcaaa
    17941 gcgacgagac gaacctcgtc gcctgcaagc tcgcagagca gcttttcaac gtgccgaccc
    18001 gtatcgcacg catccgggcg acggattacc tggtcaacga gcgcgtcctc ggggaggagt
    18061 gcttcgcggt gaacctggcg atatgtcccg agcaagtgct gactgactac atcgtcaagc
    18121 tggtccagtt cccggaggcc ttgcaggtgc tcgagttcgc tcacggcaag gtgagcctcg
    18181 tcgcggtgcg agcgttccag ggcggaccgc tcgtgggcca tcagctcaag gacatccgca
    18241 gccacctgcc gtcggtgaac acgcgcatcg cggcgatttt ccgtggcgac agtcctatcg
    18301 tgcccgaggg agacaccgtc gtcgatgcgg gcgacgaggt cttctgcctg gcagcgacgc
    18361 gcgatatacg gcatgtgatg cgcgagctcc gtcgcatgga caagcccgtc cagcgcatga
    18421 tgatcgccgg cggcgggaac atcggcctca ggctcgcggg cgcgctcgag cgcgactact
    18481 ccgtcaagat catcgagtac agcaagaagc ggtgcgagct gctatcgagc cggctcaacc
    18541 gcgcgctcgt gctgcagggc gacgtgacgg acgaggcgct gctcgaggcg gaaaatgtcg
    18601 acgacatgga catgttcatc gcggtcacca acgacgacga gaacaacatc atgtccgcgc
    18661 tgctcgcgaa gcggatgggc gcgaagcgcg tggtggcgct catcaaccgc cgttcgtacg
    18721 tggacctgct gcagtccggc cagatcgaca tcgccatctc gcccgcacag gcgaccatcg
    18781 gcacgctgct cgcgcacgtg aggcgcggcc atgtgacgca ggtgcactca ctgcgccgag
    18841 gcgccgccga ggcgctcgag gcggtcgtgc acggtgatcg cgaatcctgc cgctgcatcg
    18901 gccggcggat cgacgagatg gatctcccca agggggcgac cattgccgcc atcgtccgcg
    18961 gcgacgaagt catcatggcg catcacgaca cgatcatcca ggccgaggac cacgtcatca
    19021 tattcgtcac cgacaagaag acgctgccga gagtggagaa gctgttccag gtcggcgtgg
    19081 gattcgtgta gccgcggcat gatccggctg cttcccgtgc tcagcgtgct gagcgtgacg
    19141 atcgccggct tcgcggtcgc gatgctggtc ccgctcggga tctcgatcct ctacgcggac
    19201 ggcgcccagg gcgcctatga cgaggccgtc gtcgccacct tcctcgccgg gatcgccctg
    19261 tgggttctta cgcgaaaaca ccggacggaa ctgcaggcgc gcgacggctt cctgctgatg
    19321 gccctggtct ggacggtgct ccccgcgttc gcgacgattc cgctcctggt gtacatcaaa
    19381 gggctcgcgt tcaccgatgc gtacttcgag accgtggccg gcctcacgac gacgggcgcg
    19441 accatactct ccgggctgga ccagctgccg ccgtccatca atttctggcg ctgcgagctc
    19501 cagtggctcg gcggcatggg catcatcgtg ctggccgtcg cgattctgcc gctgctcggc
    19561 gtcggcggca gccagatcat gcgtgccgag actcccgggc ccatgaagga aacgaagctc
    19621 acgccacgga tcaccgagac cgcgaaagga ttgtgggtgg tttatgcggg gctcacggtg
    19681 ctgtgcatgc tggtgatgaa gttcgcaggc atgtcgtggt tcgacgccgt gctgcacgcc
    19741 ttttccaccg tcagcctcgg cgggttctct tcgcacgacg agaacttctc gtttttccgc
    19801 tcgccgggga ttgaagccgt cacgattttc ttcatggcga tcgccggcat gaactttggc
    19861 acccattttc tcgccttccg ccgccggtcc gcgggcccgt accgccggga tccggaggca
    19921 ggcgcctacc tcgctgtact cggactctcg gtgctcggga tcgcctattt tcttttcgcg
    19981 cacgaggtgt acccctcgtt ctggaccgcg ctgcgctatt cggcgttcaa cgtcgtatcg
    20041 atcgcgacga cggcgggata cgccagcacg gacttctacc aatggccgat cttcgcgccg
    20101 gtatggatgc tgttcctgtg ctgcttcacg acctgctccg gctcgaccgg cagcggcatc
    20161 aagctgatcc gggcggagct catggtgcgt caggcgttgc gggagatggt ccggatcgtc
    20221 cacccgcgcg cgtacgtgcc cgtgaagctc ggcggcgcga cgatcgagaa caacatcatc
    20281 ttcgcggtgc tcgcattcat gttgatctgg ggggggtcgg tgatcgcgat gacgatgctt
    20341 ctcaccgcgt cgggtgtaga catcatcacg gcgttctcgg cggtcgtggt ctgtatctgc
    20401 aacacgggcc cgggcctcaa ccaggtgggc cccggagcga cctacgcggc gctcggcgac
    20461 ttcccgactt ggatcctcac gttcgcgatg ttgctcgggc gcctcgagct gttcacgttg
    20521 ttcatcgtgc tgacgcccgc gttctggcgc aagtgaaggc ggctcagggg gccggcaaga
    20581 gccggaacga cgagaagaaa gtgtccgcgt cggccgcgcc gaggcgctcc tttctcgcga
    20641 tcagcgcaac ctgataaagc ttgtcgtccg cgaccagcac gcgcgcgatc agcgtcgtcg
    20701 ttgcgccctc ggcctcgaac tctcgtccca gcgcgcggcc gaccgcgatc tggcgatccg
    20761 cgacgatgcg ccctgagatg ttggcggcga gggcgtcccg ggtgctcgcg acgagcgcgc
    20821 gtcccgcctt gggcgcgttc gcgtagccca cgccgtacag ggcgccgccc gcacgcgcgg
    20881 accagagatg catcaccacg ccgggacggc ccgagagctc ccgctgctcg aaggtgggcc
    20941 tgcccggaag gacggccacg aaggcgcctt cgtcggaccg gaactcgtgc cagtcgagct
    21001 cgggggtgca ggctgcggcg ctcagtgcga gcgccgcgag cgcgatgcgc acgcgacgca
    21061 ggcgatcagc gctcgtacgc gagcacgggt gcgagccagc gctcggtctc atcgaccttc
    21121 acgagcttgc ggcgcgcata atcatgcgcc tggtcctcgc cgatcttacc tacggcgaaa
    21181 tagcgagcct cgggatgcgc gagatagaag ccgctgacgc tcgccgccgg aagcatggcg
    21241 tagctctccg tcagcgtcat gcccgcgtcc tgcgcacgga gaagcgcgaa cagagggcct
    21301 ttctcggtat ggtcgggaca cgcgggatat cccggcgcgg gtcgtatgcc gcgatacctc
    21361 tcggcgatca gggcgtcgct gccgaggtcc tcatccggcg catagcccca gagttcgcgc
    21421 cgcacgcggg cgtgcatttg ctcggcaagg gcctcggcga gacggtccgc cagcgccttg
    21481 agcatgatgg caccgtagtc gtcgtgcttc gcttcgaatt gcgcgacgtg ctggtcggcg
    21541 ccgacgccgg cggtcacggc aaaggcgccg atgtaatcgc gcacggggcc gtcgcggggc
    21601 gcgacgaagt ccgccaggca gaggttgaag cggtccccgg ctttctcggc ctgctgccgc
    21661 aggcaatgcc agaccatggc agtgtccgcg cgggactcgt cgcggtagat ctcgatgtcg
    21721 tcgccgacgg cggccgcagg aaacagtccg acgaccgcat ttgccgtgag ccacttttcc
    21781 gagatgatct tcgcgagcat cgcctgggcg tcggccagga cgtttcgcgc ggcctgtccg
    21841 acgacagggt cctcgagtat cccgggatag ctgccggcca ggtcccaggt ctggaagaac
    21901 ggcgaccagt cgatgaggcg tgcgatctcc gccagatcgc atcccgcgaa cactcggcgc
    21961 cccaaccatt tcggcttcgt cggctggtac tgctgccagt cgacgcggag cgcattcgcc
    22021 cgtgccgcgt ccaggctgac cagcgcctgg cctttcttgg ccgcgtgctg cgcgcgtatc
    22081 ttgtcgtagt cgccctggac ggagcgtgcg tattctgcct tctgctccga aaggaggctc
    22141 gagcacacgg acaccgagcg cgacgcatcg ggaacgtaca cggtcggccc cgaatattgc
    22201 ggcgcgatct tcaccgcggt gtgcacgcgc gacgtggtgg cgccgccgat gagcagcggc
    22261 acggtgaacc cttggcgacg catttcggcg gcgacgtgcg ccatctcctc gagcgacggc
    22321 gtgatgagac cggagagtcc gatgatgtcg gccccctcgg acttggcagt gtcgaggatc
    22381 ttctgcgccg gcaccatcac gccgagattg acgacctcga agttgttgca ctggagcacg
    22441 acgctcacga tgttcttgcc gatatcgtgc acgtcgccct tcacggtcgc gatgacgatc
    22501 ttgcccttgg atctcgaatc gccggccttc agcttgtccg cctcgatgta gggcacgagg
    22561 taggcgaccg cctgcttcat cacgcgcgcc gacttgacca cctgcggcag gaacatcttc
    22621 cccgagccga acaggtcgcc gactacgttc atcccgtcca tcaacgggcc ttcgatcacc
    22681 tggatcggac gctcgcactt gagccgcgcc tcctccgtgt cctcgacgat ccactcgttg
    22741 atgcccttca cgagcgcgtg caccagtcgc tcttccacgg gacgcgcccg ccacgcgagg
    22801 tcctcggcct gcgtcctggc ggcgcccttc acggtggtcg cgaactcgac gagccgctcg
    22861 cccgcgtcgg gacggcggtt gaggaccacg tcctcgacgc gctcgcgcag ctcggccgga
    22921 atctcctcgt agacgccgag ctggccggcg ttcacgatgc ccatggtcat cccggcccgc
    22981 acggcgtgat agagaaagac cgtgtggatc gcctcgcgga cgggatcgtt gcctcggaag
    23041 gagaacgaga cgttggatac gccgcccgac accttgacaa aggggagcga gcgccggatc
    23101 tcgcgcacgg cttcgatgta gtcgaggcca taattgttgt gctcctcgat cccggtggcg
    23161 atcgcgaaga tgttcgggtc gaagatgatg tcctcgggcg cgaaccccgc tttttcgatc
    23221 aggatgccgt agcagcgcct gcagatgtcg atgcgccgct gcagcgagtc ggcttggccg
    23281 tgctcgtcga aggccatcac gaccactgcg gccccgtagc ggcgcgccag gagcgcgtga
    23341 cgcacgaact ccgcttcgcc ctccttcagc gaaatcgagt tcacgatgca cttgccctgc
    23401 acgcatttga gaccggcctc gatgaccgac cacttgctcg agtcgatcat cacgggcacg
    23461 cgcgagatgt ccggctcgga ggcgatcagc gcgaggaagg tggtcatcgc cttctccgag
    23521 tcgagcatcg cctcgtccat gttgacgtcg atgatctgcg cgccgttctc gacctgctgc
    23581 ctcgccacgg tgagcgcctg cgcataatca cccgcgagga tcatcctcgc gaaggccttc
    23641 gacccggtca cattggtccg ctcgccgacg ttgacgaaga gcgacttgtc gcccacgttc
    23701 agcggctcga ggccggaaag ccgcagctcg tgctccggtc gtggcggaac gcgcggtttc
    23761 acgccccgga cctcggcggt gatggccgcg atgtgctccg gcgtggtgcc gcagcagcct
    23821 ccgacgatgt tgacgagccc gctttcggcg aactcgtgca gcaggcagga agtgtgcgcg
    23881 ggcccttcgt catacccggt ttcggaaagc gggttgggca gacccgcgtt cgggtagcag
    23941 ctgatatagg tctcggcgac cttcgcgagc tcctcgatgt acggccgcat cagctgagcg
    24001 ccgagcgcgc aattgagacc gaccgcgagg gggcgcgcgt gacgcaccga gttccagaag
    24061 gcctccgggg tctggcccgt gagggtgcgg ccggattggt ccgtgatcgt gcccgagatg
    24121 atcaccggca agcgtccgcc gatccgttcg aacagcgtct cgatcgcgaa gatcgccgct
    24181 ttggcgttca acgtgtcgaa gatggtctcg aggagcagga ggtcgacgcc accttcgacg
    24241 agcgcgctcg ctgcctcgcc gtacgtccgc acgagctcgt cgaacccgat gttgcggaac
    24301 cccgggtcgt tgacgtcggg cgagatcgag gccgtcttgt tggtcgggcc gagcaccccg
    24361 gcgacgaatc gcgggcgacc gggtgtgcgg gcgacgaact cctgcgccac ctcgcgggcg
    24421 agccgtgccg cccgcaggtt catctcgccg acgaattcct ccaatccata gtcggcctgc
    24481 gagggcgcgt tggaattgaa ggtgttcgtc tcgatgatgt ccgcgcccgc ggacagatag
    24541 ctcgcgtgga tggcgcggat caggtcgggc cgggtgatcg tgaggacgtc gttattgcct
    24601 tggaggtcgc gtccgaaacc ggcgaatttc tcgccgcgga agtcggcctc gttcagcctg
    24661 tggcgctgga tctcggtgcc catggcaccg tccaggatca ggatgcgctc gtcgaggagc
    24721 gcgcgcagtt cgtgggtacg gtccggttgc atgccaacaa aaaacccgat ctgccagcgc
    24781 gaatcgggtt tcgccgcttt agcaggactt cttggaccgg tcgctcgaaa cccgagaccg
    24841 gctggcgccc gcaagctgtc gcatcaaatc agcgccaaga tgggacacgg tagccacaat
    24901 ctaagtactt gtcaatgcag aaatctttag tgggaaaggt cctttagaac tacgcgcgct
    24961 cgggccgtca gcgcggcggc agaatcttga cgcgcagctc tcccaagccc tcgatgccgg
    25021 ccaccagctc gtcgccggcc ttcgccgcgc ccacgccggc gggagtgccg gtcatgatga
    25081 ggtcgccggc ctcgagcgtg tagtactgag tcaggaacgc gatctgctcg ggaatgctcc
    25141 agatcatctg atcgatgtcg gagtcctggc gcgtctggcc gttgacctta agccagagct
    25201 tcgccttctt cggatggccg gccctcgcgg cgggcgtgat gggtcccgcc ggcgctgcct
    25261 gatcgaagtc cttgccgaac gtccacggac gccccttgtc cttgccgacc tgctgcagat
    25321 cgcgccgcgt catgtcgagc ccgatcgcgt aaccccaaac gtggtcgttc gccttgtcga
    25381 ccgtgatctt gtagccgccg ctgccgatca cggcgacgag ctcggtctcc caatgatagt
    25441 tgccggtcat cggcgggtag tggaccgaga tggtctcgtt ggcgttggcg gggaccaggt
    25501 cgtgggctga tttcgtgaag aagaacggtt gctcgcgacc atcgccgccc atctctttct
    25561 ggtgttccac gtagttgcgc ccgacgcaga agatgcgccg caccggaaag cgcgcattgg
    25621 tgcccacaat cggcagggaa gggatttccc aggttcgaat cgcgtactcc attttcatgt
    25681 cgaggtcctc gcgaagacgg ccgccgcccg cgtgccgggc cgccgcaaaa ggggcgtatt
    25741 ttctcatgaa cgagaggcga aaaaaaacgg gcacccgagg atgcccgttc cgggtccgct
    25801 gctcgcggac gcgagcggct acatgtggta cgcggtctcg ccgtgcgagg tgatgtccag
    25861 gccctcgcgc tcttcttctt ccggcacgcg cagcccgacc agcaggtcgg cgatcttgta
    25921 cgagacatag gcgacgaagg cggtccagac gaagaccaaa ccgaccgctt tgatctggat
    25981 ccagagctga gcccagatgc ccggatagcc ggtcttcatc gtcacccaat cctgcacgga
    26041 acccggcccg cccaggtctg gcgagttaaa gacaccggtc agcagcgcgc cgaggatacc
    26101 gccgagagcg tgtacgccga agacgtccag cgagtcgtcg gcgccgagca gcttcttcag
    26161 gccgttgaca ccccacagac acaccaagcc gaccgcgatg ccgatcacga atgcgcccgg
    26221 gatcccgacg ttgccggccg ccggcgtgat cgcgaccagt ccggcgaccg cacccgacgc
    26281 tgcaccgacc atgctcggct tacctttgaa gatccactcg ccgaacgtcc acgagagcac
    26341 tgcgcacgcg gtcgcgagga acgtgttcat gaacgcgagc gccgccgagc cgttggcttc
    26401 gagcgcagag cctgcgttga acccgaacca gcccatccac aggagcgccg cgccgatcat
    26461 ggtcatcgtt acgctgtgcg gcgtcatcgc ttccttgccg aagccgatgc gcttgccgac
    26521 cacgtaagcg ccgaccaggc cggcgacgcc cgcgttgatg tgcacgaccg tgccacccgc
    26581 gaagtcgagc gcgccccatt gccagatcag ccccgccttg gcggtcaccg cgtccacagt
    26641 cttcggatcg gtgaacgcgt cgggacccgg gaagaaccag accatgtgtg cgatcggtgc
    26701 gtagctgaac gtgaaccaga gcaccatgaa aatgagcacg gcactgaagc gcacgcgttc
    26761 gacgatggcg ccgaggatca ggcagcacgt gatcgccgcg aaggttgcct ggaacgcgac
    26821 gtacaccagc tcgtaaagcg gggtgttctt gctgaacgtg gccgccatcg agaagtcgcc
    26881 cttgatcggg tcgaacgtgc ctttgaggaa caggcgttcg gtcaagctgc cgaagaacga
    26941 attcccctcg gtgaatgcca ggctgtaccc gtacacgcac cagatcaccg tgatcagcga
    27001 gaacacgacg aatacctgca tcagcaccga gagcatgttc ttcgaccgga cgaggccgcc
    27061 atagaacagt gcgagggccg gcacgctcat catgagcacg agcgccgtcg aggtgagcat
    27121 ccacgcgacg tcgcccttgt tcggcgtggg cgccggcgcc gcggtggccg cgggtgcggc
    27181 ggccgtcgcc gtcgcggccg gagccgggac ggcagcgggc ttgtcctgcg cgaacgtcgg
    27241 agcgcaaaga cccgcgatgc tgagcagggc gagaatggcg aaaagcttct tcatcgtgtt
    27301 ctccttcaga gagcttccgc gccggtctcg ccggtgcgga ttctgacgac ttgttcgaga
    27361 tcgaagacga agatcttccc gtccccgatc ttgccggtat tcgccgattt ctcgatcgcc
    27421 tcgatggcct ggtcgagcag cgagtccttg atcgccgcct cgaccttcac cttcggcagg
    27481 aaatcgacca cgtattcggc tccgcgatag agctcggtgt ggcccttttg ccgtccgaag
    27541 cccttgacct cggtgaccgt gatgccctgc acgccgatcg cggacagggc ctcgcgcacc
    27601 tcgtcaagct tgaacggctt gatgattgcg gtgactagct tcatgaatgt ctccgtagtg
    27661 atggctgacg ggcgcggtca gaacttgaag atggcctgga gcgcgtaggt cagcagcgtc
    27721 ttgctcatcg cgccactgga ctccgtgaac accgagttgc tcgcgcgatc ctggcggatc
    27781 tcgccgcgca cctcgaaact tgggctcggg aggtatccca gcgtcgccgt ggcttcgcgg
    27841 tacttggttc cggcgttcgc gaggaagcgc agaccgtcgg tgtccttgaa ggactcgagg
    27901 cggccggccg cgcgcagctt gggcgtgatc atgtacgtca ggtagcctgc caagccgtca
    27961 tactttgcct tgatcgttcc gccgccggat agcgatgcga agttgtcctg ctgtacgcgc
    28021 aggtattccg cgccgacgct cagcgcatcg ctgaccgtgt acgtcgcgac gagattcgcg
    28081 ctcgagcgtt tgccctcgct ggcgccgggc gtggcgattg cgttggtcgt ctccttgccg
    28141 aaatagctcg aagcaacgat cgacaggggc ttgatcggcg tgaggctcat gcccagctcg
    28201 agcgtcttcc ccttggtcgt gtcctgcagc tggtcccagc cgttgttgag gcctgcggta
    28261 aggctgaccg tgtcgctcat cgcatacacg gtgcgcagac cggtgtgcgt gaacggcacc
    28321 gagccgaaga ggatggagcg cgaaatgttg ttgttgccgg tgctcgcgat cacctccgtc
    28381 ccgtgcagcg tcacgaactt gcccgccatc agcgtgagcg ggccgctggc atactggccg
    28441 taggcctgcg tgatgtcgaa catcgacgtc gtcgtcgcgg cagcgccggg agccgcagtc
    28501 tcggggaagg catggatgac ctgagcgtcc ttgccggcgg taatgttcac cagcccgccc
    28561 gcgccttcct tcggctgctt cgcgacctga aggccgaact gatgaagcgc gaacgagttg
    28621 ttctgggagt cgaagacacg atcgctgaag ccggtttcga ggttgcggtt cgcgtgcgtg
    28681 tactgcgtat cgatccagcc gctcaccgtt accccggaag cctcgaggac cttatcgagc
    28741 gtcggcacag cggcagccga ctgcgcgagc gcggtggcgg gggccacaaa ggcggccgat
    28801 actgcggttg ctactagagc cttcttcatc gggcgttcct ttcccctggg agggttcatt
    28861 aagaccttgg tctcgtagca cggcgcgtgc cattgaaatt tccgtattat tttcatgggc
    28921 gtgtgctgag acgaccgtat gcaatcggtg tccacgcacc acgctggggc ggcgtccaat
    28981 cgctctcgca ctggttcagt gcggtagtcg caatggcgcg cggaacgtta aattcgggag
    29041 ctccatccag ggaaacgcca tggcgcaagc aaacttgctg gacgaggtcg gcgcgcgagt
    29101 gagggagatt ctcgcatcga gcccggcgaa ggacgtggag accaatctgc gcgcgatgct
    29161 cgcgtcggtg ttcgagcggc tgcagctggc gacgcgcgag cagctcgacg tgcaggagcg
    29221 cgtgctcgcc cgcacccgcg agaagctgag cacgctcgaa gcgcgcgtgg cggagctcga
    29281 ggcgcggctg cgccagcgct gaccgtgtcg ctcgccgttc tccgaagccg cgccctgacg
    29341 ggcctcgagg cgccggaggt caccgtggaa gtccatctgg ccaatggact tccgggtttc
    29401 acgatcgtcg gtcttccgga tacggaagtg aaggagagca aggaccgggt gcgcgccgcg
    29461 ctgctcaact cgcgcttcga tttcccgagc cggcggatca cggtcaatct ggctcccgcg
    29521 gagcttccca aggaaagcgg ccggttcgat cttccgattg cgctcgggat cctggcggct
    29581 tcgcagcaat tgccggcgga gccgctggcg cgctatgagt tcgccggaga gctgtcgcta
    29641 tcgggcgaga tcaggccggt gcgcggggcc ctcgccatga cctggcgcgc ggcgagcaag
    29701 ggccgcgcgt tcgtgctgcc gacggcgagc gccgcggagg cggcgctcgt cacgggcgcc
    29761 gagatctatc ccgccgactc gctgatcgcc gtttgccggc atcttcgggg cgaggtgctc
    29821 ctcggcccgt acccccgcaa tgccgccggc gcgacccggg cctcgtaccc cgacctcagc
    29881 gacgtcaaag ggcaggcgca cgcgaagcgc gcgctggagg tggcagcgtc cggaggacat
    29941 agcgtcctca tggcggggcc ccccggcacc ggcaaatcga tgctcgcggc ccgttttccg
    30001 gggctgctgc ccgaaatgac ggacgccgaa gcgctcgagt cggcggcgct gcaatcgctc
    30061 ggatcccccg ggttccgcct cgaacgctgg aagcagcggc cgtttcgcgc gccgcatcat
    30121 tcggcttcga gcgtcgcgct cgtcggcggc ggcggcgatc cgcggccggg ggagatctcg
    30181 ctcgcgcacc atggtgtgct gtttctcgac gagctgccgg agttcgagcg gcgcgtgctg
    30241 gaggcgctgc gcgagcctct ggagtccggg cgcgtggcca tctcgcgcgc ggcgcgccaa
    30301 gccgaattcc ccgcgagctt tcagctcatc gcggcgatga acccgtgtcc ctgcggatac
    30361 ctcggacatt acagcggacg ctgccggtgc acgcccgatc aggtcgcgcg ctaccggcgc
    30421 aagctctcgg ggccgctgct cgaccgcatc gacgtccaga tcgaagtgcc gtctctgcgc
    30481 gaggacgatc tcacgcgcct gccggaaggc gagagctcgc tcgatgtccg tgcccgggtg
    30541 caggcggcgt acgcgcggca gctggagcgc caaggcaagc cgaacgcgcg gctcgccccg
    30601 cgcgagatcg aacggctctg ccacatcggc gatgacgccg cctccctgct caagcaggca
    30661 atcgcccgca tcaacctgtc cgcgcgcgcg taccatcgcg tgctcaagct cgcgcgcacg
    30721 atcgcggact tggccggaga agcgggcgtc ggaattacgc acgtcgccga ggcgatccaa
    30781 taccgccgcc tcgaccggcc ggcctgaagc atgccgacct acgcgatcgg agacgtgcag
    30841 ggctgtttcg acgagctcag gcagttgctc gatgcagtgg gcttcgaccg ccgcaaggac
    30901 cggctctggc tggtcggtga cctggtgaac cgcgggcctc aatcactcgc cgtgctgcgc
    30961 ttcgtccgcg agctcggaag cgcggccgtg acggtgctcg gcaaccacga cttgcatctg
    31021 ctcacctacg ccgccggcct ggccaagccg cgtccggacg acaccctcga cgaggttctc
    31081 gccgcgccgg atcgccccga gctgatcgac tggctgcgcg gccttcccat ggtccatgtc
    31141 gaagaagacc atgtgctcgt gcacgccggg ctacttccgc aatggagcgt gcaacaggcc
    31201 gaggccctcg gccgggaggt ggaagccgcg cttcgcggag cgcagcatcg cgagttcctc
    31261 gcccggctct atggcagcaa gccggatcgc tgggacgacg agttgcgcga caccgatcgg
    31321 ctgcgcgtga tcgtcaacgc gatgacgcgc atgcgcttct gcactgcgga tggagtcatg
    31381 gaattccgga cgaagggcga ggccgccgcc gcaccgcccg gctacatgcc ttggttcgat
    31441 gtccccggcc gccggagcgc gcaccacacc gtaatctgcg gccactggtc ggcgctgggg
    31501 ctgcgcgtcg cgccgacgct cctcgccctc gactcgggct gcgtctgggg cgggcgcctt
    31561 accgcggtga ggcttgaaga ccgtgcgatc actcaggtcg agtgccggac ctcggcggcc
    31621 tcgcaatccg gagcgcgccg gcgatagcgc tctcggcggc ctcggccacg gcgcggcgct
    31681 cgccgccggc ggcgccgaat cgcggcgcgt aagtgacctc gaccacgatc cgacgcgcgg
    31741 acacggtgcc ccacagcgcg ccgagaaacg tagtctcccc gacgtacgcc gcggcatcgg
    31801 tgcgtctgcc ggacgcgtca aggtagcgca acgccaccgg ctgtatagtc gccccggcgt
    31861 cgatcgccgg ctggaagagc gcggcgtgga agcgctcgag cgtatcgccg aaggtggtcg
    31921 tgccctccgg gcagatcgcg accagtgttc cgtcgacgag ctctcccgcg atagcctcgc
    31981 gagcggcccg ggcgccggcc cgcgtgcccc tttcgatgta gagcgtaccg accagggtgc
    32041 acagccatcc ggccacgggc caggaccgga tctccgactt cgcgacgaag gtcgcgggaa
    32101 ggatggcgtc gatcacgaag atatcgagcc aggaaatgtg attgagtgcg accatgcagc
    32161 gtcgcggcag ctcgggtgtg ccgctcttct cgcgcaccgc caccgacagg atcgaaagca
    32221 ggctctgtga ccagcggcgc ttctcgtcgg aacgctgctc gggcgacagg cgtggatacc
    32281 ggaaccgcgc gataagcagg ccgcggatga ggtggacgca aagtcgcgcg agccgcgcga
    32341 gccgcagcca taccggcgtc ttgttggccg gcatgtcttc cgccggcagc ctagaaatct
    32401 cgtccgaaat ggcgccgata ctccgaggcg atcgcatcac ggtcgagcca gtgattctgg
    32461 ccgccgcgct ccgaaatctt caaggagccc atgagcgacg caagctgtcc cgtgctgcgc
    32521 cagtcgcgtc ctgccgcgat gccgtagaga agccctgcac ggtaagcgtc gccgcagccg
    32581 gtcggatcga ccacggcctt cgccggcacg cacgggatgt cgatgcgctc gccgtccgcg
    32641 tagatcaccg agccctcggc gccccgcgtc aggatgagcg ccttcaccat gccggcgagc
    32701 gcctgcaggc tcttgccggt acgctcctcg agcagcttgc cctcgtagtc gttcacggcc
    32761 acgtaagtgg cctgatccac gaagcggagc agctccttgc ccgagaacat cggcagcccc
    32821 tgccccgggt cgaacatgaa cggaatccgg tgctcggcga gctcgccggc
//
LOCUS       DATDMW010000269         6045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_703888, whole genome shotgun sequence.
ACCESSION   DATDMW010000269 DATDMW010000000
VERSION     DATDMW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703888"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VI319_11720"
     CDS             <1..591
                     /locus_tag="VI319_11720"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY6864555.1"
                     /translation="DSVGVNLWGRAEDDPVSVDEMLIVCAAVRRGAKRILVSCDVPAG
                     YGEAVAASLRLAREGGADMVKLLADPATVTAVVRAGVPVFAEFHGGRDAPIEKLVDEA
                     KRLEDAGASLLDFRHSGPVAGPAVVRAVSIPVIGGLGGGPWLDGRVRLAHTAIGYAPK
                     WLDAKSETYANTAKLALAAFAALADDVRAGRQIKGD"
     gene            662..955
                     /locus_tag="VI319_11725"
                     /pseudo
     CDS             662..955
                     /locus_tag="VI319_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1287..2642)
                     /locus_tag="VI319_11730"
     CDS             complement(1287..2642)
                     /locus_tag="VI319_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6864556.1"
                     /translation="MGTGSPRILIAGAGIGGLTAALALLRLGYDVDVYEQAHELKEVG
                     AGLNISANGNRVLHALRVLDQLEPLACELRGKDLRLWNTGRSWRIIDFGEESIERYGY
                     PYLAVWRPDLLGVLAAAIRAEKPDAIHLGSECAGFVQDEAGVTLHLGDGRRATGHALI
                     GADGVHSTIRRALFGEDRPEFTGIVAWRATVPMRLLPGHMARRVSTNWVGEGAHVVHY
                     PVRRGEMMNFIGSVERADWKVESWSAVGTAEECAEHYRGWHEDIQLLIRSAPSLYKWA
                     LLSRAPMTRWTLGRVSLLGDACHPTLPTLGQGAAMAMEDGFVLAMCLELSDGDVPLAL
                     ERYEDARRNRTRKVVLGSAENARRFHDHALATAEAAQAYVDREWSRERVFKRYDWLYS
                     YDVSRTIEWTRARETRAAARSPLPRSARREARPSTGTSAGIPRGSARPRSRAKRRGPG
                     R"
     gene            2723..3937
                     /locus_tag="VI319_11735"
     CDS             2723..3937
                     /locus_tag="VI319_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864557.1"
                     /translation="MSKLSRRDILKAGTAGAAVLGFPAILRAQQKEIVILGLWDQTGA
                     YADVGPLNDRGMRMALEERGMKILGKPIKYITRDGATQAGVSTRRAEEAIDEGARFMI
                     GPWSSGVALAVSEVAKRRKVLHLFSGGTEDISGARCHRYSFQWAASPYTAAKTVVDNF
                     MKANPKAKRWHLLIADYAFGWSLEKYIKEVGKDHGLEFVGADRHPLGEREFSNYVQKA
                     AANRPDAVAMVNFGADAVGAAREFFNFGLTPKVPLIMSWSSGVEELIQLAPEIRENLW
                     VGTNFYYTVDTPVAKRFAASYEAKFKTPPGYAPSSAYSMTRMLLAGFDKAKSDDTQAV
                     IRALETLQINDVVGPMRVNGATHQTLRPYFFLRCKPKSAMRNELDLADIVATGDTPLP
                     QEYASCKDIGAF"
     gene            3951..4808
                     /locus_tag="VI319_11740"
     CDS             3951..4808
                     /locus_tag="VI319_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6864558.1"
                     /translation="MTLLAGQILNGIATGMLYALMGLGLALIAGILNIPNFAHGALFA
                     VGAYLTVSAIGATGNFWLALVVAPLGVALLGLAIEYAGIRRLYAAGHDYQLLFTFGLS
                     LILAETIILIWGPVGISQLPPPSLRGGVDLGFTFYPKYRLFVMAAAGLLVVGTWLFLE
                     KTRYGAIMRAGIENKEMVSLLGIDIHRLFTVAFALGAWLAGVAGALTAPIRGLSPGMG
                     VDMLGIAFVVVALGGMGNVLGAIAAGLLIGVAQAVVAAYWTEASVAVIYLVMAAVLVV
                     RPQGLFGIR"
     gene            4805..5731
                     /locus_tag="VI319_11745"
     CDS             4805..5731
                     /locus_tag="VI319_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6864559.1"
                     /translation="MNARNLALLAAVILLPLFVRPALAAEIWIFAIFALGCNLLLGYT
                     GLLSFGQSTFFGSAAYVAGWMLKHWGVNLFVALTAGTGVGALSALAVGYLCVQRSGIY
                     FIMLTFALNQLFYFVAYQWTSVTGGEDGMPGIPRPAMLGIDFSDPVAYYALVSLLFLA
                     SLAVMKRIVDAPLGRILQAIRENETRAEAVGYDVARFKLLAFVIAGAFSGAAGVLYAT
                     LFGIVPLEAIGFVFSGNVVFATLIGGSGSLYGPIIGSVVFIWLSESVSTVWARWPLLL
                     GVAFVVVVLYFRGGVVEAWSRFLSWRSSRRAA"
     gene            5704..>6045
                     /locus_tag="VI319_11750"
     CDS             5704..>6045
                     /locus_tag="VI319_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6864560.1"
                     /translation="MALLETRGLTKAFGALTAVDGVGLAIEAGSLHSVIGPNGAGKTT
                     LFNLLTGTFPPDSGSIVLDGRDITGAPAHRVAHLGLARSFQRTNVFPAFSLLDNVWIA
                     AFATGRSRRGLM"
BASE COUNT          824 a         2119 c         2123 g          979 t
ORIGIN      
        1 gactcggtcg gcgtcaacct ctgggggcgg gccgaggacg atccggtgtc ggtcgacgag
       61 atgctgatcg tgtgcgccgc cgtgcgccgc ggcgcgaagc gcatcctcgt gagctgcgat
      121 gtgcccgcgg gctacggcga ggcggtcgcc gcctcgctgc gcctcgcgcg cgagggcggc
      181 gccgacatgg tcaagctcct cgcggatccc gcgaccgtga ccgccgtcgt gcgcgccggc
      241 gtcccggtgt tcgccgagtt ccacggcggc agggacgctc cgatcgagaa gctcgtcgac
      301 gaggcgaagc ggctcgagga cgcgggcgcg tcgctgctcg acttccggca ctccgggccc
      361 gtcgccgggc cggcggtggt gcgcgcggtc tcgatcccgg tcatcggcgg cctcggcggc
      421 ggaccgtggc tcgacggccg cgtgcgcctc gcgcacacgg cgatcggcta cgcgccgaag
      481 tggctcgacg cgaagagcga gacttacgcc aacacggcga agctcgcgct cgccgcgttc
      541 gccgcgctcg ccgacgacgt gcgcgcgggc cgccagatca agggcgactg agatgccgtt
      601 cgcgacgatc gacggcatcc gcacgcgcta cgaggtgatc ggcagcggcc cgccgctcct
      661 catgtactcg cccgcgggct tcgacgcgac gctcgacaag tggacgaccc agggcgtgta
      721 cgcgcgcatc cggctgctcg atcacctgcc gaagcgatac acctgcatcg tcttcgaccg
      781 gcgcgagacc ggacagtcgg gcgggcgcgt cgagcgcatc ggctggagcc actacgtcgc
      841 gcagggccgc ggcctgctcg atcatctcgg cttcgaccgc gcgcacctgc tcggcggctg
      901 catgggctgc tgcccggtcg gcgccttcgc cgtggcgcat cccgagcgcg tcctgagcat
      961 gatcctgtac tggccggtgg gcggcgccaa ataccggatc agcacgcaca agcgcttcgc
     1021 cgatcacctc gagtacgtcg accgggaagg cctcgcgggc gtggtgtcgc tggtgatgaa
     1081 agagggaaag cctttcaacg ccgagccccg gggcggcccg tgggcatcgg tgatcaaaaa
     1141 cgacccggct ttcgcgcagg cttacgccgc gctcgacgcg gtgaagtacc aggcgctcgt
     1201 ctcggagatg tgcgggacgc tgttcgaccg cgacacctcg ccgggcgcgg agcccgagga
     1261 catgctccgc tgcgagatcc cggcgctcat cgtcccgggc cgcgacgctt cgcacgcgac
     1321 ctcggccgcg cgctacctcg aggaatgcct gccgaagtcc cagtactggg acgcgcttcc
     1381 cgacgcgcag accgaggcaa cggcgagcgc gcggctgctc gagtttctcg agcgcgcgtc
     1441 cactcgatag tgcggctcac gtcgtagctg tagagccagt cgtagcgctt gaacacgcgc
     1501 tcgcggctcc attcgcggtc gacgtacgcc tgcgcggcct cggcggtcgc gagcgcgtgg
     1561 tcgtggaagc gccgcgcgtt ctcggccgag ccgagcacga ccttgcgcgt gcggttgcgg
     1621 cgcgcgtcct cgtagcgctc gagcgcgagc ggcacgtcgc cgtcggagag ctcgaggcac
     1681 atcgcgagca cgaagccgtc ctccatcgcc atcgccgcgc cctgcccgag cgtcggcagc
     1741 gtcgggtggc aggcgtcgcc gagcaggctc acgcggccga gggtccagcg cgtcatcggc
     1801 gcgcgcgaca ggagcgccca tttgtagagc gagggcgcgc tgcggatcag gagctggata
     1861 tcctcgtgcc agccccggta gtgctcggcg cattcctcgg ccgtgccgac ggcgctccag
     1921 gactcgacct tccagtcggc gcgctccacg ctgccgatga agttcatcat ctcgccgcgg
     1981 cgcacgggat agtgcaccac gtgggcgccc tcgccgaccc agttcgtgct cacccggcgc
     2041 gccatgtgcc cggggaggag gcgcatgggc acggtcgcgc gccacgcgac gatgccggtg
     2101 aactccggtc ggtcctcgcc gaagagcgcg cggcggatcg tcgagtggac gccgtcggcg
     2161 ccgatgagcg cgtgcccggt cgcgcgccgg ccgtcgccga gatgcagcgt caccccggcc
     2221 tcgtcctgga cgaagccggc gcactccgag ccgagatgga tcgcgtcggg cttctcggcg
     2281 cgtatcgcgg cggcgagcac gccgagcagg tccgggcgcc agaccgcgag gtaaggatag
     2341 ccatagcgct cgatcgactc ttcgccgaaa tcgatgatgc gccaggaccg gccggtgttc
     2401 cacaggcgca ggtccttgcc cctcagctcg caggcgagcg gctcgagctg gtcgagcacg
     2461 cgcagcgcgt gcagcacgcg attgccgttg gcgctgatgt tgagcccggc gccgacctcc
     2521 ttcagctcgt gcgcctgctc gtagacgtcg acgtcgtagc cgagcctgag gagcgcgagg
     2581 gcggcagtga gaccgccgat gcctgcaccg gcaatcagaa tccgcgggct ccctgttccc
     2641 acggacctgc atcttggacg cgtcctgacg tccggtcaat aggggtgata ggctgcgggc
     2701 tttcgccacc ccgggaggca ccatgagcaa gctcagccgc agggacatcc tcaaagcggg
     2761 cacggccggc gcagcggtcc tcggcttccc cgcgatcctc cgcgcgcagc agaaggagat
     2821 cgtgatcctg ggcctgtggg accagaccgg cgcctatgcc gacgtcggcc cgctgaacga
     2881 ccgcggcatg cgcatggcgc tggaagagcg cggcatgaag atcctcggca agccgatcaa
     2941 gtacatcacc cgcgacggcg ccacccaggc cggcgtgtcg acgcgccgcg ccgaggaggc
     3001 gatcgacgag ggcgcgcgct tcatgatcgg cccctggtcc tcgggcgtgg cgctcgcggt
     3061 gtcggaggtg gcgaagcgtc gcaaggtgct ccatctcttc agcggcggca ccgaggacat
     3121 ctcgggcgcg cgctgccacc gatactcgtt ccagtgggcg gcgagcccct acaccgcggc
     3181 gaagaccgtg gtcgacaatt tcatgaaggc caacccgaag gcgaagcgct ggcacctgct
     3241 catcgccgac tacgccttcg gctggtcgct cgagaagtac atcaaggaag tcggcaagga
     3301 ccacggcctc gagttcgtcg gcgccgaccg ccatccgctc ggcgagcgcg agttctcgaa
     3361 ctacgtgcag aaggccgcgg ccaaccgccc cgacgcggtg gcgatggtga acttcggcgc
     3421 cgacgcggtc ggcgcggcgc gcgagttctt caacttcggc ctcacgccga aggtgccgct
     3481 gatcatgtcg tggtcctcgg gcgtcgagga gctgatccag ctcgcgcccg agatccgcga
     3541 gaacctctgg gtcgggacga acttctatta cacggtcgac acgccggtcg cgaagcgctt
     3601 cgcggccagc tacgaggcca agttcaagac gccccccggc tacgcgccct cctccgccta
     3661 ttcgatgacg cgcatgctgc tcgccggctt cgacaaggcg aaatccgacg acacgcaggc
     3721 ggtgatccgc gcgctcgaga cgctgcagat caacgacgtc gtcgggccga tgcgcgtgaa
     3781 cggcgcgacg caccagacgc tgcgcccgta cttcttcctg cggtgcaagc cgaagtcggc
     3841 gatgcgcaac gagctcgacc tcgccgacat cgtcgccacc ggcgacaccc cgctgccaca
     3901 ggagtacgcg agctgcaagg atatcggcgc gttctagagc cgggcccgct ttgacgctgc
     3961 tcgccggcca gatcctcaac gggatcgcga cggggatgct ctatgccctg atggggctgg
     4021 ggctggcgct gatcgcgggc atcctgaaca tcccgaactt cgcgcacggg gcgctgttcg
     4081 cggtcggcgc gtatctcacg gtgagcgcga tcggcgcgac cggcaacttc tggctcgcgc
     4141 tggtggtcgc cccgctcggc gtcgcgctcc tcggcctcgc gatcgagtac gccgggatcc
     4201 gccggctgta cgcggcgggg cacgactatc agctgctgtt caccttcggg ctgtcgctga
     4261 tcctcgccga gaccatcatc ctgatctggg ggccggtcgg gatcagccag ctgccgccgc
     4321 cgagcctgcg cggcggcgtc gacctcgggt tcaccttcta ccccaagtac cggctgttcg
     4381 tgatggccgc ggcggggctg ctggtcgtcg gcacgtggtt gttcctcgag aagacgcgct
     4441 acggcgcgat catgcgcgcg ggcatcgaga acaaggagat ggtgtcgctg ctcggcatcg
     4501 acatccatcg attgttcacc gtcgcgttcg cgctcggcgc ctggctcgcg ggcgtcgccg
     4561 gcgcgctgac ggcgccgatc cgcgggctgt cgccgggcat gggcgtcgac atgctcggca
     4621 tcgccttcgt ggtggtcgcg ctcggcggca tgggcaacgt cctcggcgcg atcgccgcgg
     4681 gcctgctgat cggcgtcgcg caggcggtcg tcgcggcgta ctggaccgag gcgtcggtcg
     4741 cggtgatcta cctcgtgatg gcggcggtgc tcgtcgtgcg gccgcagggg ctgttcggga
     4801 tccgatgaac gcccggaacc tggccctcct cgcggcggtg atcctgctgc cgctgttcgt
     4861 gcgtccggcg ctcgccgcgg agatctggat cttcgcgatc ttcgcgctgg gatgcaatct
     4921 gctgctcggg tacaccgggc tgctctcgtt cgggcaatcg acgttcttcg gctcggcggc
     4981 ctacgtcgcc ggctggatgc tcaagcactg gggtgtgaac ctcttcgtcg cgctcaccgc
     5041 cgggacgggc gtgggcgcgc tgtcggcgct cgcggtcggg tatctgtgcg tgcagcgctc
     5101 gggcatctat ttcatcatgc tcaccttcgc gctgaaccag ctcttctatt tcgtcgccta
     5161 ccagtggacg agcgtcacgg gcggcgagga cggcatgccc ggcatcccgc ggccggcgat
     5221 gctcgggatc gacttctcgg acccggtcgc gtactacgcg ctggtctcgc tgctgttcct
     5281 cgcgtcgctc gccgtgatga agcgcatcgt cgatgcgccc ctcggccgga tcctgcaggc
     5341 gatccgcgag aacgagacgc gcgccgaggc agtgggctac gacgtcgcgc gcttcaagct
     5401 gctcgccttc gtgatcgccg gcgccttctc gggcgccgcg ggcgtgctgt acgcgacgct
     5461 cttcggcatc gtcccgctcg aggcgatcgg gttcgtgttc tccggcaacg tggtgttcgc
     5521 gacgctgatc ggcggctcgg gctccctcta cgggccgatc atcggctcgg tggtgttcat
     5581 ctggctctcc gagtcggtga gcacggtgtg ggcgcgctgg ccgctgctgc tcggcgtcgc
     5641 cttcgtcgtc gtcgtcctgt acttccgcgg cggggtcgtc gaggcctggt cgcgcttcct
     5701 gtcatggcgc tcctcgagac gcgcggcctg accaaggcct tcggcgcgct gaccgcggtc
     5761 gacggcgtcg gcctcgcgat cgaggccggc tcgctgcatt cggtcatcgg gccgaacggc
     5821 gcggggaaga cgacgctgtt caacctcctc acgggcacct tcccgccgga ttccgggagc
     5881 atcgtcctcg acggtcgcga catcacgggg gcgcccgcgc accgcgtcgc gcacctcggc
     5941 ctcgcccgct cgttccagcg cacgaacgtg ttcccggcgt tctcgctcct cgacaacgtc
     6001 tggatcgcgg cgttcgcgac cggccgctcg cgacgcggcc tgatg
//
LOCUS       DATDMW010000270         7347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1079473, whole genome shotgun sequence.
ACCESSION   DATDMW010000270 DATDMW010000000
VERSION     DATDMW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079473"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VI319_11755"
     CDS             complement(<1..1086)
                     /locus_tag="VI319_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6864561.1"
                     /translation="MKEDTPRIEVHDALASIPHDAWNALAGGHPFLRHELLRAMEETG
                     CVDEASGWAPCHLALWQADALVGAMPLYRKTHSYGEYVFDWAWADAYHRHGLEYYPKL
                     VSAVPFSPVTGPRLLARDRATRSLLVRAALQLATSASSLHVLFPPETEAEELAAHGMM
                     LRRSVQFHWMNRGYGSFEQFLSELASAKRKKIRQERRRVADAGVTLRRLVGHDIRDEH
                     WSFFVRCYNRTYRAHRSTPYLNLDFFRRLGAALPRHVLLVLAERDGQPIASALNLFSD
                     TSLWGRYWGCIAEVPMLHFETCYYQALEFCIEQGIGVFEGGAQGEHKLARGFLPVQTA
                     SAHWLKHPGFADAVEKFLERESAGVDRY"
     gene            1176..2870
                     /locus_tag="VI319_11760"
     CDS             1176..2870
                     /locus_tag="VI319_11760"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY6864562.1"
                     /translation="MGQALKIAIAQINCVVGDLAGNVEKILEFGRRANAAGAELMITP
                     ELALCGYPPEDLLLREGFYRDCAAALADLAARAPRGLTVLVGHPHEHEGKKYNAASVV
                     QEGRVLATYHKRTLPNYTVFDEQRYFQPGEESCVFDLAGLPFGVNICEDTWGPRGAAM
                     TRPDPVNVGVNICADAWEAEAPRAARAAGARVLLVLNASPYHMRKQESRYDVMRERVA
                     ENGMALVYANLVGGQDELVFDGASFVLDSRGEATHQLPAFEEALGIVTIRDGDPVPGE
                     KAPDLAVEAEVYKALCLGVRDYVTKNGFPGVLLGLSGGVDSALTLCIACDALGAGRAR
                     AVMMPSQYTADMSLEDSRALVRALGVRYDEIPIKPAFDAFLATLAREFAGRPVDATEE
                     NLQARIRGTLMMALSNKFGSLVLTTGNKSEMAVGYATLYGDMAGGFAVIKDIAKTLVY
                     RLCRYRNSVAPVIPERILTRAPSAELRPNQTDQDSLPPYDVLDAIIAAYMENDMSPGE
                     IVAQGCSPADVRRVVEMIHRNEYKRRQAPIGIRITHRGFGKDWRYPVTSRYRERVE"
     gene            2916..3254
                     /locus_tag="VI319_11765"
     CDS             2916..3254
                     /locus_tag="VI319_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY6864563.1"
                     /translation="MKKIEAVVKPFKLDEVREALAEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVTDKMLDSAIEAIVKSARTGKIGDGKIFVTAVEQVVRI
                     RTGETDEAAV"
     gene            complement(3251..3796)
                     /locus_tag="VI319_11770"
     CDS             complement(3251..3796)
                     /locus_tag="VI319_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY6864564.1"
                     /translation="MRKRPVHEDDAAAFREAVRGAVPLRGSERARGEPPRHPPVPVQS
                     LLDVHDALVQSGTGPIPWEQSMETGEELAYLRPGLGNDVLKKLRRGHWVTQDELDLHG
                     VTRVQAHPLVSEFLARCAKRGVRCVRIVHGKGLRSPNREPVLKGRIRTWLARRDDVLA
                     FCQAPAAEGGGGAMLVLLKGR"
     gene            complement(3809..4756)
                     /gene="trxB"
                     /locus_tag="VI319_11775"
     CDS             complement(3809..4756)
                     /gene="trxB"
                     /locus_tag="VI319_11775"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY6864565.1"
                     /translation="MSKKNKHCRLLILGSGPAGYTAAVYAARANLKPVLVTGLAPGGQ
                     LMTTTDVDNWPADADGVQGPELMERFQKHAERFETEIVFDHVHTAHLAERPIRLEGDA
                     SDYTCDALIIATGASARYLGLPSEQSYMGKGVSACATCDGFFYKGEDVAVVGGGNTAV
                     EEALYLANIARNVTVVHRRDKFRAEKIMQDRLFARDNVSIRWDHVLDEVVGDASGVTG
                     VRIKNARTGAPDELRVKGLFIAIGHTPNTGIFEGQLDMKDGYIITHGGNQGNATATSV
                     PGVFAAGDVQDHVYRQAVTSAGSGCMAALDAEKYLEALG"
     gene            4909..5583
                     /locus_tag="VI319_11780"
     CDS             4909..5583
                     /locus_tag="VI319_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY6864566.1"
                     /translation="MATQASVSTLLLRNVPLFAVLTENQLELLTGVVSRKSFPRGTTI
                     IAAGDMTDSLYVIISGRLKVMMTDDDGGEVILAILGPNEFFGEMGLLDDAPRSASVVA
                     IEPCELLSLSKKDFKKCMADNFEMTMTVMRGVVKRLREADKKIGSLALMDVYGRVARL
                     LLEMSETIDGQKVVTKKLAKQDIAKMIGASREMVSRVMKDLQTGGFIEVKGGSIFLKD
                     NILAVD"
     gene            5648..>7347
                     /locus_tag="VI319_11785"
     CDS             5648..>7347
                     /locus_tag="VI319_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY6864567.1"
                     /translation="MKKSQAPLPGKVAALLRESKWFVLIAVGLYLALVFATFNKADPG
                     WSHSAAGVEITNAGGRVGAWIADVLLFLFGVSAWWWVGLFAYAVAWGYRRLDGSSLSD
                     RRSFIVAAVGFAVLLLASSGIEALRMHWIRAALPSVQGGMVGSEIARLLWVTFGLTGA
                     TLILLTLCAVGLSLFTGVSWITVVEVIGAALEWAYTQALERWQGWQDRKAGEIAAVRR
                     EAIVGEKREQLIEAHPPIRIEPPVIEIPKSARVQKEKQEPLFRELPDTPLPPIKLLDE
                     ATSDVETLSAETLEYTSRLIEKKLADFGVQVKVLAAYPGPVITRYEIEPAVGVKGAQI
                     VNLVKDLARALSVYSIRVVETIPGKSCMGLEIPNPHRQTVRLAEIVSSQAYHELHSPL
                     TLALGKDIAGNPVVTDLTKMPHLLVAGTTGSGKSVAINAMILSMLYKSEPRDVRLILV
                     DPKMLELSMYQDIPHLLAPVVTDMKQAANALSWCVGEMERRYKIMSWVGVRNLSGYNH
                     KVAEAEKAGAPLMDPNTLEGTEPVPLEKLPYIVVIIDELADLMMVSGKKVEELVARLA
                     "
BASE COUNT         1189 a         2468 c         2548 g         1142 t
ORIGIN      
        1 gtagcggtcc acgccggcgg actcgcgctc caggaatttc tcgaccgcgt ccgcgaatcc
       61 cgggtgcttc agccagtgcg ccgaagccgt ctgcaccggg aggaagccgc gggcgagctt
      121 gtgctcgccc tgcgctccgc cctcgaacac gccgatgcct tgctcgatgc agaactcgag
      181 cgcctggtag tagcacgtct cgaagtgcag catcggcacc tcggcgatgc atccccagta
      241 gcgtccccag agcgacgtgt cggagaacag gttcagcgcg ctcgcgatcg gctggccgtc
      301 gcgctcggcg agcaccagca gcacgtgccg cggcagggca gcgcccaggc ggcggaaaaa
      361 gtcgaggttc aaatagggcg tcgagcggtg cgcgcggtag gtgcggttgt agcagcggac
      421 gaagaacgac cagtgctcgt cgcggatgtc gtgcccgacg agccgccgca aggtcacgcc
      481 ggcgtccgcg acccggcgcc gctcctgccg gatcttcttg cgcttcgcgg atgcgagctc
      541 cgagaggaac tgctcgaagc tcccgtatcc gcgattcatc cagtggaact gcaccgaccg
      601 gcgcagcatc atgccgtgag cggcgagctc ctcggcctcg gtctccggcg ggaacagcac
      661 gtgcagcgac gacgccgacg tcgcgagctg cagcgccgcg cgcacgagca gcgagcgcgt
      721 cgcgcggtcg cgcgcgagca agcgcgggcc ggtgaccggc gagaagggaa ccgccgatac
      781 gagcttcggg tagtactcga gcccgtggcg atgataagcg tccgcccagg cccagtcgaa
      841 gacgtactcg ccgtacgagt gcgtcttgcg gtagagcggc atcgcgccga cgagcgcgtc
      901 ggcctgccac agcgcgagat gacaaggcgc ccagccgctc gcctcgtcga cgcatccggt
      961 ctcctccatc gcgcgcagca gctcgtggcg caggaacggg tggccgccgg cgagggcgtt
     1021 ccacgcgtcg tgcgggatgc tcgcgagcgc gtcatggacc tcgattcgcg gcgtgtcttc
     1081 cttcatgcgc ggttgagcga tgccatgccc gattatagga ggcgcgttcc ggacgtcgag
     1141 acgtgggccg cgatcatgga tggtagactt ggacgatggg tcaggcgctg aagatcgcga
     1201 tcgcgcagat caactgcgtc gtcggcgacc tcgcaggcaa cgtcgagaag atcctcgagt
     1261 tcggccgtcg cgcaaatgcc gccggtgccg agctcatgat cacgcccgag ctcgcgctgt
     1321 gcggatatcc gcccgaggac ctgctcctgc gcgaaggctt ctaccgcgac tgcgccgcgg
     1381 cgctcgcgga cctggcggcg cgcgcgccgc gcggcctcac cgtgctcgtc ggccatccgc
     1441 acgagcacga aggcaagaag tacaacgccg cctcggtcgt gcaggaggga cgcgtgctcg
     1501 cgacctacca caagcgcacg ctcccgaact acacggtgtt cgacgagcag cgctacttcc
     1561 agcccggcga ggagagctgc gtcttcgacc tggccgggct gccgttcggc gtgaacatct
     1621 gcgaggatac ctggggcccg cggggcgcgg cgatgacgcg cccggacccc gtcaacgtcg
     1681 gcgtcaatat ctgcgccgac gcctgggaag cggaggcgcc gcgagcggcc cgtgccgccg
     1741 gcgcgcgcgt gctgctcgtc ctgaacgcat cgccctatca tatgcgcaag caggaatcgc
     1801 gctacgacgt gatgcgcgag cgcgtcgcgg agaacggcat ggcgctcgtc tacgccaacc
     1861 tggtgggcgg gcaggacgag ctcgtgttcg acggcgcctc gttcgtgctc gacagccgcg
     1921 gcgaggcgac gcaccagttg cccgcgttcg aggaggcgct cggcatcgtc acgatacgcg
     1981 acggcgaccc ggtccccggg gagaaggcgc ccgacctcgc ggtcgaagcc gaggtgtaca
     2041 aggcgctgtg cctcggcgtg cgcgattacg tgacgaagaa tggcttcccg ggcgtgctgc
     2101 tgggcctttc cggcggcgtc gactcggcgc tcaccttgtg catcgcgtgc gacgctctcg
     2161 gcgccgggcg ggcccgcgcg gtgatgatgc cgtcgcagta caccgccgac atgagcctcg
     2221 aggactcgcg cgcgctggtg cgcgccctcg gcgtgcgcta cgacgagatc ccgatcaagc
     2281 ccgcgttcga cgccttcctc gcgacgctcg cgcgcgagtt cgcggggcgc ccggtcgacg
     2341 ccaccgagga gaacctgcag gcgcgcatcc gcggcactct catgatggcg ctgtcgaaca
     2401 agttcggctc gctggtcctc acgaccggca acaagtcgga gatggcggtc ggctacgcca
     2461 cgctttacgg cgacatggcc ggcggcttcg ccgtgatcaa ggacatcgcc aagacgctcg
     2521 tctaccgcct gtgccggtac cgcaactcgg tggcgccggt catccccgag cgcatcctga
     2581 cgcgcgcgcc gagcgccgag ctgcggccca accagaccga ccaggactcg ctgccgccgt
     2641 acgacgtcct cgacgcgatc atcgcggcct acatggagaa cgacatgagc cccggcgaga
     2701 tcgtcgcgca gggctgctcg cccgccgacg tgcgccgcgt cgtggagatg atccaccgca
     2761 acgagtacaa gcggcgacag gcgccgatcg gcatccgcat cacgcaccgc gggttcggga
     2821 aggactggcg ctatccggtc acctcgcgct accgcgagcg ggtcgaatga ggggtttgct
     2881 ataatcgtgc gcacgtttca ggcgggagac cggacgtgaa gaagatcgaa gccgtggtga
     2941 agccgttcaa gctcgacgag gttcgcgagg cgctggccga ggtgggcgtc accgggctca
     3001 ccgtcaccga ggtcaagggc ttcggccgtc agaagggcca taccgagctc taccgcggcg
     3061 ccgagtacgt cgtcgacttc ctgccgaagg tcaagatcga ggtcgtcgtc accgacaaga
     3121 tgctcgactc ggccatcgag gcgatcgtca agtccgcgcg cacgggcaag atcggcgacg
     3181 gcaagatctt cgtcaccgcg gtggagcagg tggtgcgaat ccgcaccggc gagaccgacg
     3241 aggccgcggt ttagcgccct ttcagcagca ccagcatcgc cccgccaccg ccttcggcgg
     3301 ccggcgcctg gcagaaggcg agcacgtcgt cgcggcgcgc gagccaggtc cggattctcc
     3361 ccttgagcac cggctcgcgg ttcggcgagc gcaacccctt gccgtggacg atgcgcacgc
     3421 agcgcacgcc gcgcttggcg cagcgcgcga ggaactcgga gacgagagga tgcgcctgga
     3481 cgcgcgtcac gccgtgcagg tcgagctcgt cctgcgtgac ccagtgaccg cggcgtagct
     3541 tcttcagcac gtcgttcccc agcccgggcc gcagatacgc gagctcctcg cccgtctcca
     3601 tcgactgctc ccacgggatg gggccggtgc cgctctggac gagggcgtcg tgcacgtcga
     3661 ggagcgattg caccggcacg ggcgggtgtc gcgggggctc gccacgcgcg cgctcggagc
     3721 cccgcagcgg aaccgcgccg cgcacggcct cgcggaacgc cgcggcgtcg tcctcgtgga
     3781 ccggacgctt gcgcacgtcg tgctccgctc agccgagcgc ctcgagatac ttctcggcgt
     3841 ccagcgcggc catgcagccg ctgcccgcgc tcgtgaccgc ctgtcggtag acgtggtcct
     3901 ggacgtcgcc cgcggcgaac acgcccggga cgctggtcgc ggtcgcgttc ccctggttgc
     3961 ctccgtgggt gatgatgtag ccgtccttca tgtcgagctg gccctcgaag atgcccgtgt
     4021 tcggcgtatg gccgatggcg atgaacagcc ccttcacgcg cagctcgtcc ggcgcgccgg
     4081 tgcgggcgtt cttgatgcgc acgccggtga cgccgctggc gtcgccgacc acctcgtcga
     4141 ggacgtgatc ccagcggatc gacacgttgt cgcgcgcaaa aaggcggtcc tgcatgatct
     4201 tctcggcccg gaacttgtcg cgacggtgca cgacggtcac gttgcgcgcg atattggcga
     4261 ggtagagggc ctcctcgacg gcggtgttgc cgccgccgac cacggcgacg tcctcgccct
     4321 tgtagaagaa gccgtcgcag gtcgcgcacg ccgagacgcc cttgcccatg tagctctgct
     4381 ccgagggaag gccgagatag cgcgccgagg cgccggtcgc gatgatgagc gcgtcgcagg
     4441 tatagtcgga cgcatcgccc tcgagccgga tcgggcgctc cgcgaggtgc gccgtgtgga
     4501 cgtggtcgaa gacgatctcg gtctcgaagc gctcggcgtg cttttggaag cgctccatca
     4561 gctcgggacc ctggacgccg tcggcatcgg ccggccagtt gtcgacgtcc gtcgtcgtca
     4621 tcagctggcc gccgggcgcc agcccggtga cgagcacggg cttcaggttg gcgcgcgcgg
     4681 cgtagacagc ggcggtgtag cccgccggcc cggagccgag gatcagcaga cggcaatgct
     4741 tgtttttctt cgacatagat cggacaatgc ctcggcggcc tcgcggaaac gaaattatag
     4801 cgcgggggcg ccgcggttcc gccgcggcag ccggccctct cgcgatagat gaggtctggt
     4861 aacataagag acaagaggat gaccctgaga acgggggcgg ggaggatcat ggcaacacag
     4921 gcgagcgtat ccacgctgct tctgcggaac gtcccgctgt tcgcggtgct gactgagaac
     4981 cagctcgagc tgctgaccgg cgtcgtcagc cgcaagagct ttccgcgcgg cacgaccatc
     5041 atcgccgcgg gcgacatgac cgactcgctc tacgtcatca tctccgggcg cctcaaggtc
     5101 atgatgaccg acgacgacgg cggcgaggtg atcctcgcga tcctggggcc caacgaattc
     5161 ttcggcgaga tgggcctgct cgacgacgcg ccccggtccg cgagcgtcgt cgcgatcgag
     5221 ccgtgcgagc tgctcagcct ctccaagaag gatttcaaga agtgcatggc cgacaacttc
     5281 gagatgacca tgaccgtcat gcgcggcgtc gtgaagcggc tgcgcgaggc cgacaagaag
     5341 atcggcagcc tcgccctcat ggatgtgtac ggccgtgtgg cacgcctgct gctcgagatg
     5401 tccgagacga tcgacggcca gaaggtcgtc accaagaagc tcgccaagca ggacatcgcc
     5461 aagatgatcg gcgcgtcgcg cgaaatggtg agccgcgtga tgaaggatct ccagaccggc
     5521 ggcttcatcg aggtcaaggg cggatcgatc ttcctcaagg acaacatcct cgcggtcgat
     5581 tgacggcacg ccgcgaccgg gaggcgcgcg gcttggagca ccatgaactc gtggcgttcg
     5641 tgagtttgtg aagaagagcc aggcaccgct cccgggcaaa gtcgccgcgc tgctgcgcga
     5701 atccaagtgg ttcgtcctga tcgcggtcgg actctatctc gctctcgtct tcgccacctt
     5761 caacaaggcc gaccccggct ggtcgcacag cgccgcgggc gtcgagatca ccaatgccgg
     5821 gggccgcgtc ggcgcctgga tcgccgacgt gctgctcttc ctgttcggcg tgtcggcgtg
     5881 gtggtgggtc ggcctgttcg cctacgccgt cgcctggggc taccggcgtc tcgacggcag
     5941 ctcgctcagc gaccggcgct cgttcatcgt cgccgcggtc gggttcgcgg tgctgctgct
     6001 cgcctcgagc gggatcgagg cgctgcgcat gcactggatc agggccgcgc tgccgtcggt
     6061 ccagggcggg atggtcggca gcgagatcgc gcgccttctc tgggtcacgt tcggcctcac
     6121 cggcgcgacg ctgatcctgc tcaccttgtg cgcggtcggc ctctcgctct tcaccggcgt
     6181 gtcctggatt accgtggtcg aggtgatcgg cgcggcgctc gaatgggcgt acacgcaggc
     6241 gctcgagcgc tggcaggggt ggcaggaccg caaggcgggc gagatcgccg ccgtccggcg
     6301 cgaggcgatc gtcggggaga agcgcgagca gctgatcgag gcgcacccgc cgatccgcat
     6361 cgagccgccc gtgatcgaga taccgaagtc ggcgcgcgtg cagaaggaga agcaggagcc
     6421 gctgttccgc gagctgccgg acacgccgct gccgcccatc aagctgctcg acgaggcgac
     6481 gtccgacgtc gagacgcttt ccgccgagac gctcgagtac acgtcgcggc tcatcgagaa
     6541 gaagctcgcc gacttcggcg ttcaggtgaa ggtcctcgcg gcctacccgg gcccggtgat
     6601 cacgcgctac gagatcgagc ccgcggtcgg cgtcaagggc gcgcagatcg tgaacctggt
     6661 gaaggacctc gcccgcgcgc tgtcggtgta cagcatccgc gtggtcgaga ccatcccggg
     6721 gaagtcctgc atgggcctcg agatccccaa tccgcaccgg cagaccgtgc gcctcgcgga
     6781 gatcgtcagc tcgcaggcgt accatgagct gcattcgccg ctcacgctcg ccctgggcaa
     6841 ggacatcgcc ggcaacccgg tcgtcaccga cctgacgaaa atgccgcacc tgctggtcgc
     6901 gggcaccacg ggatccggga agtcggtcgc gatcaacgcg atgatcctct ccatgctgta
     6961 caagtcggaa ccgcgcgacg tgcgcctcat cctggtcgat ccgaagatgc tcgagctgtc
     7021 gatgtaccag gacattccgc acctgctggc gccggtcgtg accgacatga agcaggcggc
     7081 gaacgcgctt tcctggtgcg tgggcgagat ggagcggcgc tacaagatca tgtcgtgggt
     7141 cggcgtgcgc aacctctcgg gctacaacca caaggtcgcc gaagcggaaa aggcgggcgc
     7201 tccgctcatg gatcccaata cgctcgaggg caccgagccg gtgccgctcg agaagctgcc
     7261 ctacatcgtc gtcatcatcg acgagctggc cgatctgatg atggtatccg ggaagaaggt
     7321 cgaggagctg gtcgcgcgtc tggcgca
//
LOCUS       DATDMW010000271         7892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2074345, whole genome shotgun sequence.
ACCESSION   DATDMW010000271 DATDMW010000000
VERSION     DATDMW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074345"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VI319_11790"
     CDS             <1..817
                     /locus_tag="VI319_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY6864568.1"
                     /translation="AYASTINDTDPTLSVEPRNTRGSTENIPLLEAGGIDIGQATGEV
                     TYEALQGIGRPKAELRIVNAMYASFGMFAVRADSPARTIADLRGKPIAWGASGSGFVV
                     LARYVMDGLGLDLERDFQPIYLERAGDGSAMVLDGRAAALWGGGIGWPNFVTLAKAGA
                     RFVAPTPDDVRRVQAKHVFIKTIAVPPGSYPGQDAAIAAPGSWSLVLASPRLADDVAY
                     RLVRALHRGRGAIAARHPQGHESTLENTLAAAVTRELVHPGVLRYMREAGILR"
     gene            complement(819..3356)
                     /locus_tag="VI319_11795"
     CDS             complement(819..3356)
                     /locus_tag="VI319_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6864569.1"
                     /translation="MQALRSWWNEQRSRPNYGEFAQAVIRVGIGLALACYYAAMGSWQ
                     PAWIPAFFWGFVATTFVLLAWVSALRRPSPFVRLLSMVVDIGTPTVLLGFTGERSAII
                     LFVYTWVPVGHGFRFGLSYLRAAWVVSILGFVAVYGISAAVDGYWYGHPMVWLGAMIW
                     IAAPTIYVALLLKQKLVAVQTAERAQAEAERERVERARVQAERARADAEAASEAKSEF
                     LATMSHEMRTPLNGVVGAAEMLATKELPPAERQLVDWLLASSRQLRSLIDNLLDLRKI
                     EAGKMEIEPAPFDLHVLMNRVAALFEPEAKRARLRFTKSVAADAPYLLIGDDARIQQV
                     LINLVANAIKFTRDGSVRVSVGALTQAPERIILHFEVRDTGIGIEPEHAARIFERFTQ
                     ANTGIHRQFGGSGLGTTICKHLVELMGGSINFGSRPAGGTTFWFTVPLGRQPASAEAA
                     GAEPALRDARLYFISTRAAAGELMAGAARRWRVAHARFASLADFLGAARSDAQYGPRV
                     LIVDGEDSTCAWRDVPDALEHAGVALPCILAHPGIGENEAFDAGYASRVDGRDERLLA
                     AALRSVVAGAPGAGAVAEFRLPAPLGRTGLRILVAEDNAISQQIIAMMLRSGGHQVTL
                     VDDGESVLEQYRNVAFDIAVLDMHMPRSSGLEVAREIRRIEASGGVRRMPIVMLTAAA
                     STDLREDSLDAGIDLFLSKPIDPRALLQGVQQAFAHSGGDVQEGAPAEARDGYVDRDL
                     LKDMAALAPDAGFVRRLASRFAEDSYRLVDRLEAALERGDQREFRELAHALKGAAMMT
                     GAIRLRDSAARLETASKSALAAVDPGAVRELRRTLEATHQALAAAVE"
     gene            3460..4188
                     /locus_tag="VI319_11800"
     CDS             3460..4188
                     /locus_tag="VI319_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864570.1"
                     /translation="MSGSGPDVLLGAFRARPADAPVHVLGLRGASVVIVDDEDTARTV
                     LDHMVRGIDPEVSTSVFAGGDEALDWLKGHEADLVITDYRMPGMNGAQLAARLRQEAR
                     LRHVPVMVVTATNERAVRYEALEAGAVDFLAKPIDPIEVRTRCRNLLLLTRQYRLVKD
                     YSTLQERKLEQLQDLLRPLLRGSGTKDVAERLEQGELVTLGFEKLFAITSCIAGVQEL
                     VDAAQRHIGELEEQLIRPLRRPDD"
     gene            complement(4192..4584)
                     /locus_tag="VI319_11805"
     CDS             complement(4192..4584)
                     /locus_tag="VI319_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6864571.1"
                     /translation="MTQEARATRARIPKRGLGLGSAVRRLRELHGVGQEVLAERSGVS
                     QGYLSQIESGTWTTVTEDVLGRIANALGVEPWMILAQAAGLKLDQVERFTDDERRWLD
                     VYRALDPEQRETVLRVATVMRRPPARRR"
     gene            4705..5436
                     /locus_tag="VI319_11810"
     CDS             4705..5436
                     /locus_tag="VI319_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEY6864572.1"
                     /translation="MDLHAGEVLAAPTITTLQQSLTRSVRSLGYRYFIYRGWFAQPGG
                     AGRDVYIDDCPPGWRDYCSRQIHERRDPLQAQAFQGSKPMPWARLAALAPEFYARARD
                     FGLVTGTSHPVHGPGAQWSSLTFVCDRLGHDPALTPSITLGGCQLLATYAHDAVARIL
                     VRERGGRPARTGALGAGFALNEREREILGLAAAGHTNAGIAGLLSISDRTVVFHLRNA
                     REKLGAANCVHAVSKALSLGLIASA"
     gene            complement(5433..6335)
                     /locus_tag="VI319_11815"
     CDS             complement(5433..6335)
                     /locus_tag="VI319_11815"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6864573.1"
                     /translation="MSINAPAPPPNCAPPDFRPRRPRIALPEGACDCHAHILGPARAH
                     PYAPQRVYTPPDCLLPDYERMLAALGLERAVLVQPSVYARDNTVLLEALARGGGRYRG
                     VAVVSPEIQDAELEQLDAQGVRGVRVNVVDVPGRRPGALPLEELRTLARRVAPLGWHL
                     ELLLHVDEFPELDRMLAGFPADVVLGHLGYMKTGRGVADAGFRALLRLLGAGRCWVKL
                     TGPYRLSAAPLPYADVIPFARALAEARPDRLLWGSDWPHVMLKGAMPNDADLIELLTD
                     WLPGDALRRRVLAENPAALYGFAG"
     gene            6454..>7892
                     /locus_tag="VI319_11820"
     CDS             6454..>7892
                     /locus_tag="VI319_11820"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY6864574.1"
                     /translation="MGRFDVASFLSTLGSSADELPAQMQLAQELIETLPVPVFFKARD
                     GRYLGVNRAWEEFFGFTREEFLGKTVHQLYPQSPGVAEKHFAMDLELWQNPGSQRYEI
                     PVTVRGGRVRHTIYCKATFTGADGTVAGLIGTILDITERKQAEQREAIEHAATRLIAE
                     ADTPSDAIRSIIAAVCRQLGWVCGAHWTLQPGAKTLKRAETWTNGDPAIGTFLDATQG
                     MVYAPSHSGLVRRVLGTAMPVWIGDVATEKEFLRAQSAAAAGLHGAFALPVLIGNEVR
                     GVIEFYGSDVAHFDDWLLQLGMSLGNQIGQLLARRQAEADLREAHEELSRRAQDLARS
                     NAELQQFAYVASHDLQEPLRMISSYTQLLMRRYGKLFDQDAKEFMEFIVDGAARMKQL
                     IEDLLAYSRVGTRGRDLEPTDAHGALDQALANLRAAIDASGAAVTHDPLPTVRADASQ
                     LTQLFQNLVANALKFRGAEAPRVHVLARE"
BASE COUNT         1141 a         2853 c         2832 g         1066 t
ORIGIN      
        1 ggcctacgcg tcgacgatca acgacacgga cccgacgctc tccgtcgagc cgcgcaacac
       61 caggggcagc accgagaaca tcccgctgct cgaggcgggc gggatcgaca ttggccaggc
      121 gaccggcgag gtcacctacg aagccctgca gggcatcggc cggccgaaag ccgagctccg
      181 catcgtcaac gcgatgtacg cgagcttcgg catgttcgcg gtgcgcgcgg acagcccggc
      241 gcggacgatc gccgaccttc gaggcaagcc gatcgcctgg ggcgcgtcgg gctcgggctt
      301 cgtcgtgctc gcgcgctacg tgatggacgg cctcgggctc gacctcgagc gcgacttcca
      361 gccgatctac ctcgagcgcg cgggcgacgg ctccgcgatg gtgctcgatg gccgagccgc
      421 ggcgctctgg ggcggcggca tcggctggcc gaacttcgtc accctcgcga aggcgggcgc
      481 ccgcttcgtc gcgccgacgc ccgacgatgt gcggcgcgtc caggcgaagc acgtcttcat
      541 caagacgatc gcggtgccgc cgggcagcta cccggggcag gacgccgcga tcgccgcgcc
      601 cggctcgtgg tcgctcgtgc tcgccagccc gaggctcgcc gacgacgtcg cgtatcgcct
      661 cgtgcgcgcc ctgcaccgcg gccgcggcgc gatcgccgcg cgccacccgc agggacacga
      721 gtcgacgctg gagaacacgc tcgcggccgc ggtgacgcgc gagctcgtgc atcccggcgt
      781 gctgcgctac atgcgcgagg ccgggatcct gcgctgactc actcgaccgc ggcggcgagc
      841 gcctggtgcg tcgcctcgag cgtgcggcgc agctcgcgca ccgcaccggg gtcgaccgcc
      901 gcgagcgccg acttgctcgc cgtctcgagg cgcgccgccg agtcgcgcaa ccggatcgct
      961 ccggtcatca tcgcggcccc cttcagggcg tgcgcgagct cgcggaactc gcgctggtcg
     1021 ccgcgctcga gcgccgcctc gagccggtcg acgagtcgat acgagtcctc ggcgaagcgc
     1081 gacgcgagcc ggcggacgaa ccccgcgtcc ggggcgagcg ccgccatgtc cttgagcagg
     1141 tcgcggtcga cgtagccgtc gcgcgcttcc gccggcgcgc cttcctgcac gtcgccgccc
     1201 gagtgcgcaa acgcctgctg cacgccctgc agcagcgcgc gcggatcgat gggcttggac
     1261 aggaacaggt cgatgccggc gtcgagcgag tcctcgcgca gatcggtgct ggctgcggcc
     1321 gtcagcatga cgatcggcat gcggcgaacg ccgcccgacg cctcgatacg ccggatctcg
     1381 cgcgccacct cgaggccgct cgagcgcggc atgtgcatgt cgagcaccgc gatgtcgaag
     1441 gcgacgttgc ggtactgctc gaggacgctc tcgccgtcgt cgaccagggt aacctggtgg
     1501 ccgccgctgc gcagcatcat ggcgatgatc tgctggctga tcgcgttgtc ctccgcgacc
     1561 aggatgcgca ggccggtgcg gccgagcggc gccggcagcc ggaactcggc gaccgcgccc
     1621 gcgcccggtg cgccggcgac gacgctgcgc agcgcggcgg cgagcaggcg ctcgtcgcgc
     1681 ccgtccaccc gtgaggcata gccggcgtcg aaggcctcgt tctccccgat gccagggtgc
     1741 gcgaggatgc acggcagcgc cacgcccgcg tgctcgagcg cgtcagggac gtcccgccac
     1801 gcgcacgtgg agtcctcgcc gtcgacgatc aggacgcgcg gcccgtactg ggcatcgctg
     1861 cgcgcggcgc cgaggaagtc ggcgagcgag gcgaagcggg cgtgggcgac gcgccagcgc
     1921 cgcgccgcgc ccgccatcag ctcgcccgcc gcggcgcgcg tggagatgaa gtacaggcgc
     1981 gcatcgcgca gcgcgggctc cgcgcccgcg gcctccgcgg aagcgggctg gcgcccgagg
     2041 ggcaccgtga accagaacgt cgtccccccg gccggccggc tgccgaagtt gatgctcccg
     2101 cccatcagct cgaccaggtg cttgcagatc gtggtcccca ggcccgagcc gccgaactgg
     2161 cggtggatgc ccgtgttcgc ctgcgtgaag cgctcgaaga tgcgcgccgc gtgctcgggc
     2221 tcgatgccga tgcccgtgtc gcgcacctcg aagtgcagga tgatgcgctc gggggcttgc
     2281 gtcagcgcgc cgacgctcac gcgcaccgat ccgtcgcggg tgaacttgat ggcgttggcc
     2341 acgaggttga tcagcacctg ctggatgcgc gcgtcgtcgc cgatcagcag gtagggcgcg
     2401 tccgccgcga cgctcttggt gaagcgcagg cgcgcgcgct tcgcctcggg ctcgaacagg
     2461 gcggcgacgc ggttcatgag cacgtgcagg tcgaaaggcg cgggctcgat ctccatcttc
     2521 ccggcctcga tcttgcgcag atcgagcagg ttgtcgatca gcgagcgcag ctgccgcgac
     2581 gacgcgagca gccagtcgac cagctggcgc tccgccggcg gcagctcctt ggtcgcgagc
     2641 atctcggcgg cgccgaccac gccgttgagc ggcgtgcgca tctcgtggct catggtcgcg
     2701 aggaactcgc tcttcgcctc gctcgcggcc tcggcgtcgg cgcgcgcgcg ctcggcctgc
     2761 actcgggcgc gctcgacgcg ctcgcgctcc gcctcggcct gggcgcgctc ggcggtctgc
     2821 accgcgacca gcttctgctt gagcagcagc gcgacgtaga tggtgggcgc ggcgatccag
     2881 atcatcgcgc cgagccacac catcgggtgc ccgtaccagt agccgtcgac cgcggcgctg
     2941 atgccgtaga ccgcgacgaa gccgaggatc gacacgaccc aggcggcgcg caggtagctc
     3001 aggccgaagc ggaagccgtg ccccaccggc acccaggtgt agacgaagag gatgatcgcg
     3061 ctgcgctcgc ccgtgaagcc gagcagcacc gtcggcgtgc cgatgtcgac caccatcgac
     3121 agcaagcgca cgaacggcga aggccgccgc agggccgata cccacgccag cagcacgaag
     3181 gtcgtggcga cgaagcccca gaagaacgcg gggatccacg cgggctgcca actccccatc
     3241 gcggcgtagt agcaggcgag cgcgagcccg atcccgacgc ggatcacggc ctgcgcgaac
     3301 tcgccgtagt tcggccgcga gcgctgctcg ttccaccacg accgcaaggc ctgcaccccg
     3361 tccctcccgc gcggcgccat ggcttgtaga tacgccgcaa aaatattagc atggcgatca
     3421 ggctcgcgcg ccgcgcggcc gccacccgag gtgcctcgca tgagcggctc cggccccgac
     3481 gttctcctcg gcgcgttccg cgcccggccc gccgacgctc cggtccacgt gctcgggttg
     3541 cgcggggcgt cggtggtgat cgtcgacgac gaggacacgg cgcgcacggt gctcgaccac
     3601 atggtccgcg gcatcgaccc ggaagtgtcg acctcggtct tcgccggcgg cgacgaagcc
     3661 ctcgactggc tgaagggcca cgaggcggat ctcgtgatca cagattaccg catgccgggg
     3721 atgaacggtg cgcagcttgc cgcccggctg cgccaggaag cgcgcctgcg ccacgtcccg
     3781 gtgatggtcg tgaccgcgac gaacgagcgc gccgtgcgct acgaggcgct cgaggcgggc
     3841 gcggtcgatt tcctcgccaa gccgatcgac ccgatcgagg tacgcacgcg ctgccgcaac
     3901 ctgctgctgc tcacgcggca gtaccgcctg gtgaaagact actcgacgct gcaggagcgc
     3961 aagctcgagc agctgcagga cctgctgcgc ccgctgttgc gcggcagcgg cacgaaggac
     4021 gtcgccgagc gactggagca gggagagctg gtgacgctcg gcttcgagaa gctgttcgcg
     4081 atcacgagct gcattgccgg ggtgcaggag ctggtcgacg ccgcccagcg ccacatcggc
     4141 gagctcgagg agcagctcat ccggccgctg cgccggcccg acgattgacc gctagcgccg
     4201 gcgcgccggc ggccgccgca tcaccgtcgc gaccctgagg accgtctcgc gctgctcggg
     4261 gtcgagcgcg cggtagacgt cgagccagcg ccgctcgtcg tcggtgaagc gctcgacctg
     4321 gtcgagcttg aggccggcag cctgcgcgag gatcatccag ggctcgacgc cgagggcgtt
     4381 cgcgatgcgg ccgaggacgt cctcggtgac cgtggtccag gtcccgctct cgatctgcga
     4441 gaggtagccc tggctcacgc cgctgcgctc ggcgagcacc tcctgtccga cgccgtgcag
     4501 ctcgcgcagc cggcgcacgg cgctgccgag ccccaggccg cgcttgggta tccgcgcgcg
     4561 cgtggcacgc gcctcttgcg tcatcgtcgt cttgccctca cggagtcgga tcttactcta
     4621 tgcttgacac aaatattatg tacgtatatt aatacgtccg cagtcgtttc actacgtccg
     4681 cagttcgccc ccagggaggg agccatggac ctgcacgccg gcgaggtgct cgccgcgccc
     4741 acgatcacga cgctgcagca gtcgctcacg cgctcggtgc gcagcctcgg ataccggtac
     4801 ttcatctacc gcggatggtt cgcgcagccc ggcggcgccg gccgcgacgt ctacatcgac
     4861 gattgcccgc cgggttggcg cgactactgc tcgcgccaga tccacgagcg ccgcgacccg
     4921 ctccaggcgc aggcgttcca gggctcgaag cccatgccct gggctcggct cgcagcgctc
     4981 gcgcccgagt tctacgcgag ggcgcgcgac ttcggcctgg tcaccggcac gagccacccc
     5041 gtccacgggc cgggcgcgca gtggagctcc ctcaccttcg tgtgcgaccg cctcggccac
     5101 gacccggcgc tcacgccgtc gatcacgctc ggcggctgcc agctgctcgc gacctatgcg
     5161 cacgacgccg tcgcgcgcat cctcgtacgc gagcgcggcg gacgccctgc gcggaccggc
     5221 gcgctcggcg ccgggttcgc gctgaacgag cgcgagcgcg agatcctcgg cctcgccgcc
     5281 gccggccaca ccaacgcggg gatcgccggt ctgctgtcga tctcggaccg gaccgtggtg
     5341 ttccacctgc gtaacgcgcg cgagaagctc ggtgccgcga attgcgtgca cgcggtgtcc
     5401 aaggcgctgt cgctcgggct gatcgcctcg gcctagccgg cgaagccgta cagcgccgcc
     5461 gggttctcgg cgagcacgcg ccggcgcagc gcgtcgccgg gcagccagtc ggtgagcagc
     5521 tcgatcaggt cggcgtcgtt cggcatcgcg cctttcagca tgacgtgcgg ccagtcgctg
     5581 ccccagagca gccgatcggg gcgcgcctcg gcgagcgcgc gcgcgaatgg gatcacgtcc
     5641 gcgtagggca gcggcgccgc cgagagccga tacgggccgg tgagcttgac ccagcagcgc
     5701 ccggcgccga gcagccgcag cagcgcccgg aagccggcat ccgcgacgcc gcgcccggtc
     5761 ttcatgtagc cgaggtggcc cagcacgacg tcggccggaa agccggcgag catgcgatcg
     5821 agctcgggaa actcgtcgac atggagcagc agctcgaggt gccagccgag cggcgcgacg
     5881 cgccgggcga gcgtgcgcag ctcctcgagc ggcagtgcgc ccggccggcg gccggggacg
     5941 tcgacgacgt tcacgcgcac gccgcgcacc ccttgggcgt cgagctgttc cagctcagcg
     6001 tcctggatct ccgggctcac gacggcgaca ccgcggtagc ggccgccgcc gcgcgcgagc
     6061 gcctcgagga gcaccgtgtt gtcgcgcgca tacacgctcg gctgcaccag caccgcgcgc
     6121 tcgaggccga gcgcggcaag catgcgctcg tagtcgggga gcaggcagtc gggcggcgtg
     6181 tagacgcgct gcggcgcgta agggtgcgcg cgcgcgggac cgaggatgtg cgcgtggcag
     6241 tcgcaggctc cctcgggcag cgcgatccgg ggacgccggg ggcggaagtc cggcggcgca
     6301 cagttcggcg gaggcgcggg ggcgttgatg ctcaaagtgc ccggaatata gccaaaaagt
     6361 cgcgccgggg gggcgccccc aatttacaat ccgcccagga ctgaggatag tggcgggccg
     6421 ccccgggggg cgcgccactg gtggagggga atcttgggcc gtttcgacgt cgcgtcgttc
     6481 ttgtcgacgc tcggcagcag cgcggacgag ctgcccgcgc agatgcagct cgcgcaggag
     6541 ctgatcgaga cgcttccggt cccggtgttc ttcaaggcgc gcgacgggcg ctatttgggc
     6601 gtgaaccgcg cctgggagga attcttcggc ttcacgcgcg aggagttcct cggcaagacg
     6661 gtgcaccagc tctacccgca gagccccggc gtggcggaga agcacttcgc gatggacctc
     6721 gagctctggc agaacccggg cagccagcgc tatgagatcc cggtgacggt gcgcggcggc
     6781 cgcgtgcgcc acaccatcta ctgcaaggcg accttcaccg gcgccgacgg cacggtcgcc
     6841 gggctgatcg gcaccatcct cgacatcacg gagcgcaagc aggccgagca gcgcgaggcg
     6901 atcgagcacg ccgcgacgcg gctgatcgcc gaggccgata cgccctcgga cgcgatccgc
     6961 agcatcatcg ccgctgtctg ccggcagctt ggctgggtgt gcggggcgca ctggacgctg
     7021 cagccgggcg ccaagacgct caagcgcgcc gagacctgga ccaacggcga cccggcgatc
     7081 ggcaccttcc tcgacgcgac ccagggcatg gtgtacgcgc cctcgcacag cggcctggtg
     7141 cggcgcgtcc tcggcaccgc gatgccggtg tggatcggcg acgtcgccac ggagaaggag
     7201 ttcctgcgcg cccagtcggc ggccgcggcg ggcctgcacg gcgcgttcgc gctgccggtg
     7261 ctgatcggca acgaggtccg cggcgtgatc gagttctacg gcagcgacgt cgcgcacttc
     7321 gacgactggc tgctgcagct cgggatgagc ctcggcaacc agatcgggca gctgctggcg
     7381 cgccgccagg ccgaggccga cctgcgcgag gcgcacgagg agctctcgcg ccgcgcccag
     7441 gacctggcgc gctcgaacgc cgagttgcag cagttcgcgt acgtcgcctc gcacgacctg
     7501 caggagccgc tgcgcatgat ctcgagttac acgcagctgc tgatgcgccg ctacggcaag
     7561 ctgttcgacc aggacgccaa ggagttcatg gagttcatcg tcgacggcgc ggcgcgcatg
     7621 aagcagctga tcgaggacct gctcgcgtac tcgcgcgtcg ggacgcgcgg gcgcgacctg
     7681 gagccgaccg acgctcatgg cgcgctcgac caggcgctcg ccaacctgcg cgcggcgatc
     7741 gacgcctcgg gcgcggccgt gacgcacgat ccgctgccga cggtgcgcgc cgacgcctcg
     7801 cagctcacgc agctgttcca gaacctggtc gccaacgcgc tcaagttccg cggcgccgaa
     7861 gccccgcgcg tgcacgtctt ggcgcgcgag ga
//
LOCUS       DATDMW010000272         2315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1828228, whole genome shotgun sequence.
ACCESSION   DATDMW010000272 DATDMW010000000
VERSION     DATDMW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828228"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /gene="pgi"
                     /locus_tag="VI319_11825"
     CDS             <1..1430
                     /gene="pgi"
                     /locus_tag="VI319_11825"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY6864575.1"
                     /translation="HLADAETLRLLVALAEQADVRGWIRRMYAGEPINATEGRAVLHV
                     ALRSDASAFPEGRDVMPLVRAARERMRRFVEQVHGGDASGATGERIADVVNIGIGGSD
                     LGPRMATRALRRFRAPGSPRLHFVANIDPADLDAALEGLAPARTLFIVASKTFTTAET
                     LDNAARARRWLESGLGKNPAAAGRIDRHFAAVTANESGAREWGLAAERIFPLWDWVGG
                     RYSLWSAVGLPVALAVGMERFEELLEGARAMDAHFHTAPLEANMPVLMALLEIWAVNF
                     LGAGSRVVLPYGEDLRDFPAYLQQLEMESNGKRVDRAGRPVDYATAPVVWGASGTPSQ
                     HSFHQMLHQGTALVPADFIVFADRRGGAAHAALVANALAQSAALMEGDRTADPQRALP
                     GDRPSSVIMLERLAPEALGQLIALYEHKVFVEGVIWNINSFDQWGVEMGKRVARTLLP
                     RLLEGGDAAGLDASTRALLARLRGR"
     gene            1470..1676
                     /locus_tag="VI319_11830"
     CDS             1470..1676
                     /locus_tag="VI319_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEY6864576.1"
                     /translation="MNEDTFNISIRKFLKMVGVSSQREIEHAVEKARSSGAIRGNEKF
                     PASVTLDVPGLKLSVKFEGKIELE"
     gene            1677..>2315
                     /locus_tag="VI319_11835"
     CDS             1677..>2315
                     /locus_tag="VI319_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY6864577.1"
                     /translation="MNKPRELDATLLEELRALLGERVSTTRAVREHHGKDESYFPYAP
                     PDAVVFPESTEEVRDVVNLCRRHKTPIIPFGVGTSLEGHVLAIRGGVCVDLSRMNQVL
                     EVHEADLDVVVQAGVTRKQLNEHIRHSGLMFPIDPGADATLGGMASTRASGTNAVRSG
                     TMRENVLALKVVLADGRVITTSRRSRKSAAGYELTRLFVGAEGTLGIITELTV"
BASE COUNT          338 a          812 c          848 g          317 t
ORIGIN      
        1 agcacctggc ggacgccgaa acgctgcggc tgctggtcgc gctcgccgag caggccgacg
       61 tccgcgggtg gatccggcgc atgtacgcgg gcgagccgat caacgcgacc gagggccgcg
      121 ccgtgctgca cgtcgcgctg cgctcggacg cgagcgcgtt tcccgagggc cgcgacgtca
      181 tgccgctggt gcgcgccgcg cgcgagcgca tgcggcgctt cgtggagcag gtccacggcg
      241 gcgacgcgtc cggcgccacc ggcgagcgca tcgccgacgt ggtcaacatc ggcatcggcg
      301 gctccgacct ggggccgcgc atggcgacgc gcgcgctgcg ccgcttccgc gccccgggaa
      361 gcccgcgcct gcatttcgtg gccaacatcg atcccgccga cctcgacgcg gcgctcgagg
      421 gactcgcgcc ggcgaggacg ctgttcatcg tcgcgtcgaa gacgttcacg accgcggaga
      481 cgctcgacaa cgcggcgcgg gcgcgccgct ggctggagtc gggcctgggg aagaaccccg
      541 ccgcggccgg gaggatcgat cgtcacttcg ccgcggtcac cgccaacgag agcggcgcgc
      601 gcgagtgggg cttggcagcc gagcgcatct tccccctgtg ggactgggtc ggcgggcgct
      661 actcgctctg gtctgcggtc ggcctgccgg tcgcgctcgc ggtcggcatg gagcgcttcg
      721 aggagctgct cgaaggcgcg cgcgcgatgg acgcgcactt ccacaccgcg ccgctcgagg
      781 ccaacatgcc ggtgctgatg gcgctgctcg agatctgggc cgtgaacttc ctcggcgccg
      841 gcagccgcgt ggtcctgccg tacggcgagg acttgcgcga cttccccgcg tacctgcagc
      901 agctcgagat ggaatcgaac ggcaagcgcg tcgaccgcgc cggccgcccg gtggattacg
      961 cgaccgcgcc ggtcgtctgg ggcgcgtcgg gcacgccgag ccagcactcg ttccaccaga
     1021 tgctgcacca gggcacggcg ctcgtgccgg ccgacttcat cgtcttcgcg gaccggcgcg
     1081 gcggcgcggc gcacgcggcg ctcgtcgcga acgcgctcgc gcagagcgcg gcgctcatgg
     1141 agggcgaccg caccgcggat ccccagcgcg cgctgcccgg cgaccggccc tcgagcgtca
     1201 tcatgctcga gcggctcgcg cccgaggcgc tcggccagct gatcgcgctc tacgagcaca
     1261 aggtcttcgt cgaaggcgtg atctggaaca tcaacagctt cgaccagtgg ggcgtcgaga
     1321 tgggcaagcg cgtcgcgcgc acgctgctgc cgcggctgct cgagggcggc gacgccgcgg
     1381 ggctcgacgc ctcgacgcgg gccctgctcg cgcggctgcg cgggcgctga gcgtatactg
     1441 aacggcgaga cgacggcgaa aggcgagcga tgaacgaaga caccttcaat atcagcatcc
     1501 gcaagttcct gaagatggtg ggcgtcagct cccagcggga gatcgagcac gcggtcgaga
     1561 aggcgcgctc gagcggcgcg atccgcggca acgagaagtt cccggcttcg gtgacgctcg
     1621 acgtgccggg gctgaagctc tcggtgaagt tcgagggcaa gatcgagctg gagtagatga
     1681 acaagccccg cgagctcgac gcgacgctcc tggaagagct gcgcgcgctg ctcggcgagc
     1741 gcgtctcgac cacgcgcgcg gtgcgcgagc accacggcaa ggacgagtcc tacttcccct
     1801 acgcgccgcc cgacgcggtc gtctttcccg agtcgaccga ggaggtgcgc gacgtcgtga
     1861 acctgtgccg ccggcacaag acgccgatca tcccgttcgg cgtcggcacc tcgctcgagg
     1921 ggcacgtcct cgcgatccgc ggcggcgtct gcgtcgatct ctcgcgcatg aaccaggtgc
     1981 tcgaggtgca cgaggccgat ctcgacgtcg tcgtccaggc gggggtgacg cgcaagcagc
     2041 tgaacgagca catccggcat tcggggctga tgttcccgat cgatcccggc gccgacgcga
     2101 cgctcggagg catggcgtcg acgcgcgcct cgggcacgaa cgcggtgcgc tccggcacga
     2161 tgcgcgagaa cgttctcgcg ctgaaggtcg tgctcgccga cggccgggtg atcacgacct
     2221 cgcggcgttc gcgcaagtcc gcggcgggct acgagctgac gcgcctcttc gtcggcgccg
     2281 aaggaacgct cgggatcatc accgagctca cggtg
//
LOCUS       DATDMW010000273         7214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_210180, whole genome shotgun sequence.
ACCESSION   DATDMW010000273 DATDMW010000000
VERSION     DATDMW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210180"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VI319_11840"
     CDS             <1..1195
                     /locus_tag="VI319_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY6864578.1"
                     /translation="GRKNLRDLLEEWVGFRVDTVTRRTKHRLGEVERRIHILEGRKIV
                     FLNIGEVIKIIRASDEPKPALMKRFKLTDVQAEDILEIRLRQLARLEGIRIDEELKAL
                     GAERKSLKELLGSEPAMKKQVAAEIRADAKKYGDERRTRIEEAGRTVAEAKTLDEPVT
                     VIVSRNGWLRSRQGHGLDLSGVTYKEGDGPYAVFETRTIHPIAMIDSTGRAFSIQASD
                     IPGSRGDGVPFSSMVELAPGARVAHVLDAQPGRKYLVSTSAGYGFVAPSEELVTRVRA
                     GKGFMSIEEGEQILRPAPAPAGWEKGMVATVSRMGKLLLFPASEMRELARGRGLILMG
                     ADDEDPMVAAGFSDGSTVTVEGTGRGGKPKTCTVSGKELEKYKLRRARRGYLLPEKMA
                     PASIV"
     gene            complement(1213..1770)
                     /locus_tag="VI319_11845"
     CDS             complement(1213..1770)
                     /locus_tag="VI319_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY6864579.1"
                     /translation="MEAFLVSTGIVALAEMGDKTQLLALVLAARYRGRHTAVVLGILV
                     ATLVNHGLAAEVGSWLTQAIGAHAMRWIVGLSFLAMAAWILVPDKPGDDTGRAERFGA
                     FGTTLVSFFLVEMGDKTQIATVALAARYESVVAVVAGTTLGMMLANVPAVWLGDQLLR
                     RVPVRLVHVVAAALFAVLGVIALAG"
     gene            1803..3803
                     /locus_tag="VI319_11850"
     CDS             1803..3803
                     /locus_tag="VI319_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEY6864580.1"
                     /translation="MNAVLQNLRNRLAAGFGGRFYPVALVYAMTFAVGLGTRVALLAR
                     PDTQGIGGVLAGAEAFVTGAFFDAVVATYFVLPLALWLALAPSGLVRSRAHRALVVVG
                     VAAYAYLLLWVAIAEWLFWDEFGARFNFIAVDYLIYTREVLGNILESYPVGKLMSAAA
                     AGAAVVAAGLAPSIWRRIGRDATRGARLGPAGAVAAAAALAIAFVSTSARTDPGGDTA
                     RELGGNGIYQFFAAFRANSLDFPVFYATRPEAEASAFVRTRLTAAGERWPARPESSVE
                     RLVIDKAPEKRLNVVLVSEESLGAEFLGTFGNPRGLTPRLDALARESLLFTNVYATGN
                     RTVRGLEALSLAIPPTPGESIVKRPGAEGLFTLGSVFENRGYEALFVYGGYGYFDNMN
                     AYFEANDYRAVDRTMIPKERIHHENIWGVADEDLFDFAIEQFDRAYADGRPFFAHVMT
                     TSNHRPYTYPDGRIDIPSGSGRDGAVKYSDWAIGHFIDEAKKHPWFGDTLFVLTADHG
                     ANARGTLDIPVAQYRIPLLFYAPKHIEPGRVERLMSQIDIAPTLLGQLHFSYRTKFFG
                     HDLFATPPSEDRAFVANYQTLGYLRDGRLVTLMPQRRVRIAPYPVGGGAPALTEEKLR
                     DEAIAWYQLAFEAYRSRALAGDRSNREPPSAGMIRIAQPPER"
     gene            3882..4784
                     /locus_tag="VI319_11855"
     CDS             3882..4784
                     /locus_tag="VI319_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY6864581.1"
                     /translation="MSGIDRRDFLRLAGLGGAVFASGLAGCAARAGSERGVAADEFYF
                     VQLSDTHWGFSGPAVNPDAGGTLHKAVAAVNALPFQPDFIVFTGDLTHKTDDPKESRR
                     RLAEFRKIVGGLKNPNVRFLAGEHDAALDNGEAWKEQFGPSYYSFDHKGVHFVALDNV
                     SDPAARIGEKQLAWLKEDLAKHPSDAPLVVLAHRPLFDLYPQWDWATRDGAQAMEILM
                     PHRNVTVFYGHIHQENHHTTGHIAHHSAKSLIFPLPAPGSQPKREPIKWDPAQPYRGL
                     GFRSVGAEPPKPEYEITEYPVARA"
     gene            4781..5161
                     /locus_tag="VI319_11860"
     CDS             4781..5161
                     /locus_tag="VI319_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY6864582.1"
                     /translation="MISFPRRRFVAAGLTVAGAALNVTAGGGLLRAQPSPRVVRITAR
                     RFAYLPAEVTLKKGEPVTIELTSGDVVMGFNAPDFGVRADIIPGQVSRVSFTPDKAGT
                     FVFLCDIFCGDGHEGMAGKLHVTA"
     gene            5202..5747
                     /locus_tag="VI319_11865"
     CDS             5202..5747
                     /locus_tag="VI319_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6864583.1"
                     /translation="MDERKQRFERTVLPHLDAAYNLARWLTRNDHDAQDVVQEAALRA
                     FRFFDGLRGEPRPWLLAIVRNSCMSWMQINRPAELAAFDDGAVERIADDAGTPEDLAL
                     RAVDRRRLNEALAALPAPFREAIVLRELEDLAYKDIARIAEIPIGTVMSRLARGRRLL
                     ADSLRAIENAAGPNAAPKAAR"
     gene            5744..6502
                     /locus_tag="VI319_11870"
     CDS             5744..6502
                     /locus_tag="VI319_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY6864584.1"
                     /translation="MNRDEAARLLDAYADGELDPAASLELEALLARDAGLRAACERIR
                     AASAAIRAQADYHRAPAELAARLRVSIPAPPEGARAAGGLRRRRWLAPAAAFAAAAAL
                     TWTLAWTTLRPAAEERLAGDAVASHVRATLAGRLVDVESSDQHNVKPWLSARLPYSPP
                     VSDFAAEGFALRGARVDVLDGHPVAVLGYQRRRHTIDVFVRPGAGRRSGGYERDGFNV
                     ERAAADGMEFWIVSDLNREELAQLARLLSARSPG"
     gene            complement(6480..>7214)
                     /locus_tag="VI319_11875"
     CDS             complement(6480..>7214)
                     /locus_tag="VI319_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY6864585.1"
                     /translation="MAGAALLAGRAALLLGAGRVFVGFVGSAPPAVDPAQPELMLRAA
                     KDLFAKNLLTVLAAGPGLGRSEAAKKALDAAIGAKLPLVLDADGLNLVAASARLAKKV
                     AARGAPTIMTPHPAEAGRLLGRETHEVQSDRVAAAKAIAKRYSALVALKGNGTVIAAP
                     GGSWWINPTGNPGMASAGMGDVLTGIIAALLAQGASPEDAAVAGVYLHGAAADRLVAA
                     GAGPVGLTAGEVIDAARALLNPASAR"
BASE COUNT         1032 a         2613 c         2577 g          992 t
ORIGIN      
        1 cggccgcaag aatctgcgcg acctgctcga ggagtgggtc ggcttccggg tcgacaccgt
       61 cacgcggcgc acgaagcacc gcctaggcga ggtcgagcgg cgcatccaca tcctcgaagg
      121 ccgcaagatc gtcttcctga acatcgggga agtgatcaag atcatccgcg cctcggacga
      181 gcccaagccc gcgctgatga agcgcttcaa gctgacggac gttcaggccg aggacatcct
      241 cgagatccgg ctgcgccagc tcgcgcgcct cgagggcatc aggatcgacg aggagctgaa
      301 ggcgctcggc gccgagcgca agtcgctcaa ggaattgctc ggcagcgagc cggcgatgaa
      361 gaagcaggtc gccgccgaga tcagggccga cgccaagaag tacggcgacg agcgccgcac
      421 gcggatcgag gaagccggac gcacggtggc cgaggcgaag acgctcgacg agccggtcac
      481 cgtgatcgta tcgcgcaacg gctggctgcg ctcgcgccag gggcacgggc tcgacctctc
      541 gggcgtgacg tacaaggaag gcgacggccc gtatgccgtg ttcgagacgc gtaccatcca
      601 cccgatcgcg atgatcgatt cgaccgggcg cgccttctcg atccaggcgt ccgacatccc
      661 gggctcgcgc ggcgacgggg tgccgttctc gtcgatggtc gagctcgcgc cgggcgcgcg
      721 cgtggcgcac gtcctggacg cgcagcccgg caggaagtac ctcgtgtcga cgagcgccgg
      781 ctacggcttc gtcgcgccgt cggaggagct cgtcacgcgc gtgcgcgcgg gcaagggctt
      841 catgagcatc gaggagggcg agcagatcct gcggcccgcg cccgcgcccg cgggctggga
      901 gaagggcatg gtcgccaccg tctcgcgcat gggcaagctc ctgctcttcc ccgcgtccga
      961 gatgcgcgag ctcgcgcgcg gccgcgggct gatcctcatg ggcgcggacg acgaggaccc
     1021 gatggtcgcc gcgggcttca gcgacggcag cacggtcacc gtcgaaggca ccggccgcgg
     1081 cggcaagccg aagacctgca ccgtttccgg caaggagctc gagaagtaca agctgcgccg
     1141 cgcgcgccgc ggctacctgc tgcccgagaa gatggcgccc gcttctatcg tctaggccga
     1201 tcgcctggcg gcctagcccg cgagcgcgat cacgcccagc acggcgaaca gcgccgcggc
     1261 gacgacgtgc acgaggcgga ccggcacccg ccgcagcagc tggtcgccca gccacaccgc
     1321 cggcacgttg gcgagcatca tcccgagcgt cgtccccgcg acgacggcga ccacggattc
     1381 gtagcgcgcg gccagcgcga ccgtcgcgat ctgcgtcttg tcgcccatct cgaccaggaa
     1441 gaacgagacc agcgtcgtgc cgaaggcgcc gaagcgctcg gcgcggccgg tgtcgtctcc
     1501 gggtttgtcc gggacgagga tccacgccgc catcgcgagg aacgagagcc cgacgatcca
     1561 tcgcatcgcg tgcgcgccga tcgcctgcgt gagccagctg ccgacttcgg cggcgagtcc
     1621 gtgattcacc agcgtcgcga cgaggatgcc gagaaccacg gccgtgtgcc gcccgcggta
     1681 gcgcgccgcg aggacgagcg cgagcagctg cgtcttgtcg cccatttccg ccagggcaac
     1741 aatgccggtc gatacgagaa atgcctccaa ggagcgctcc gggcgccagt ataatcaacg
     1801 gcatgaatgc agttcttcag aacctccgga accgcctcgc cgcaggcttc ggcggccgct
     1861 tctacccggt ggcgctcgtc tatgcgatga ccttcgcggt cggcctcggc acgcgcgtcg
     1921 cgctgctcgc ccgccccgac acgcagggca tcggcggagt gcttgcgggc gccgaggcct
     1981 tcgtcaccgg cgccttcttc gacgccgtgg tggcgacgta cttcgtgctg ccgctcgcgc
     2041 tgtggctcgc gctcgcgccg tccggcctgg tgcgctcacg cgcgcaccgc gcgctggtcg
     2101 tggtcggggt cgcggcgtac gcgtacctgc tgctctgggt cgcgatcgcc gagtggctgt
     2161 tctgggacga gttcggggcg cgcttcaact tcatcgccgt cgattacctg atctacacgc
     2221 gcgaggtgct cggcaacatc ctcgagagtt atccggtcgg caagctcatg tccgccgcgg
     2281 cggccggcgc ggcggtcgtc gcggccgggc tcgcgccctc gatctggcgc cgcatcggtc
     2341 gcgacgcgac gcgcggcgct cggctcggcc cggcgggcgc ggtggcggcc gcggcggcgc
     2401 tcgcgatcgc gttcgtctcg accagcgcgc gcaccgatcc cggcggcgac acggcgcgcg
     2461 agctcggcgg caacggcatc taccagttct tcgccgcgtt ccgcgcgaac tcgctcgact
     2521 tcccggtgtt ctacgcgacg cggcccgagg ccgaggcgtc cgccttcgtg cgcacgcggc
     2581 tcacggccgc gggcgagcgc tggccggcgc ggcccgagtc gagcgtcgag cgcctggtga
     2641 tcgacaaggc tccggagaag cggctgaacg tcgtgctggt gagcgaggag agcctcggcg
     2701 ccgagttcct cggtacgttc ggcaacccgc gcgggctgac gccgcggctc gacgcgctcg
     2761 cgcgcgagag cctgctcttc acgaacgtgt acgcgaccgg caaccgcacg gtgcgcggcc
     2821 tcgaggcgct gtcgctcgcg atcccgccca cgccgggcga gtcgatcgtg aagcggccgg
     2881 gcgcggaggg cctgttcacg ctgggcagcg tgttcgagaa ccgcggctac gaggcgctct
     2941 tcgtctacgg cggctacggc tacttcgaca acatgaacgc ttacttcgag gcgaacgact
     3001 accgcgccgt cgatcgcacc atgattccga aggagcgcat ccaccacgag aacatctggg
     3061 gcgtcgccga cgaggacctg ttcgacttcg cgatcgagca gttcgaccgc gcctacgccg
     3121 acggccggcc gttcttcgcg cacgtgatga cgaccagcaa ccaccgcccg tatacgtatc
     3181 ccgacggccg catcgacatc ccgtcgggct cggggcgcga cggcgcggtc aagtactccg
     3241 actgggcgat cgggcatttc atcgacgagg cgaagaagca cccctggttc ggcgacacgc
     3301 tgttcgtgct caccgcggat cacggcgcga acgcccgagg cacgctcgac attccggtcg
     3361 cgcagtaccg catcccgctg ctgttctacg cgccgaagca catcgagccg gggcgcgtcg
     3421 agcggctgat gtcgcagatc gacatcgcgc cgacgctgct cgggcagctg cacttcagct
     3481 accgcaccaa gttcttcggg cacgacctgt tcgcgacgcc gccgtccgag gatcgcgcgt
     3541 tcgtcgcgaa ctaccagacg ctcgggtatc tgcgcgacgg gcgcctcgtg acgctgatgc
     3601 cgcagcggcg cgtgcgcatc gcgccttacc cggtgggcgg cggcgcgcct gcgctgaccg
     3661 aggagaaatt gcgcgacgag gcgatcgcct ggtaccagct cgcgttcgag gcgtaccgct
     3721 cgcgcgcgct cgcgggcgac cggtccaacc gcgagccgcc ctcggcgggc atgatccgca
     3781 tcgcgcagcc gccggagcgc tagccgggaa taaaggggca cggcccccgg tcttgcaggg
     3841 tgtcgccggt cgcacgaccg gcattcatcc gggggccggc catgtcgggc atcgatcgca
     3901 gggatttcct caggctcgcc gggctcggcg gcgccgtgtt cgcgtccggt ctcgcgggct
     3961 gcgccgcgcg cgcggggagc gagcgcgggg tcgccgcgga cgagttctac ttcgtccagc
     4021 tctccgatac gcactggggc ttcagcggcc cggcggtgaa ccccgacgcg ggcggcacgc
     4081 tgcacaaggc ggtcgcggcg gtgaacgcgc tgccgttcca gccggacttc atcgtcttca
     4141 cgggcgatct cacgcacaag acggacgacc cgaaggagag tcggcgccgg ctcgcggagt
     4201 tccgcaagat cgtcggcggc ctgaagaacc cgaacgtgcg cttcctcgcg ggcgagcacg
     4261 acgccgcgct cgacaacggc gaggcgtgga aggagcagtt cgggccgagc tattacagct
     4321 tcgaccacaa gggcgtgcac ttcgtcgcgc tcgacaatgt ctcggacccc gccgcgcgca
     4381 tcggcgagaa gcagctcgcc tggctcaagg aagatctcgc gaagcatccg agcgacgctc
     4441 cgctcgtcgt gctcgcgcac cggccgctgt tcgacctcta cccgcagtgg gactgggcga
     4501 cccgcgacgg cgcgcaggcg atggagatcc tgatgccgca ccggaacgtg accgtgttct
     4561 acggccacat ccaccaggag aaccaccaca cgaccgggca catcgcgcac cactcggcga
     4621 agtcgctgat ctttccgctg cccgcgcccg ggtcgcagcc gaagcgcgag ccgatcaagt
     4681 gggatcccgc gcagccttac cgcggcctcg ggttccgctc ggtgggcgcc gagccgccga
     4741 agcccgagta cgagatcacc gagtatccgg tggcgcgcgc atgatctcct tcccgcgccg
     4801 ccgcttcgtc gccgccgggc tgaccgtcgc aggggcggcc ctgaacgtga cagcgggcgg
     4861 cggccttctt cgcgcccagc cgtcgccgcg ggtcgtgcgc atcaccgcgc gccgcttcgc
     4921 gtacctgccg gccgaggtga cgctgaagaa gggcgagccg gtgacgatcg agctgacctc
     4981 gggcgacgtg gtgatgggct tcaacgcgcc cgacttcggc gtgcgcgccg acatcatccc
     5041 gggccaggtg tcccgcgtga gcttcacgcc ggacaaggcg gggacgttcg tcttcctgtg
     5101 cgacatcttc tgcggcgacg gccacgaggg catggccggc aagctgcacg tgaccgcgtg
     5161 acgtatcatg gcgcgctaga ccccggccgc ggcgacgacc cttggacgag cgcaagcagc
     5221 gattcgagcg caccgtgctt ccgcatttgg acgcggcgta caacctcgcg cgctggctca
     5281 cgcgcaacga ccacgacgca caggacgtgg tgcaggaggc ggcgctgcgc gccttccgct
     5341 tcttcgacgg cctgcgcggc gagccgcggc cgtggctgct cgcgatcgtg cgcaactcgt
     5401 gcatgagctg gatgcagatc aaccgccccg cggagctcgc cgcgttcgac gacggcgccg
     5461 tggagcgcat cgccgacgat gccggcacgc cggaggacct ggcgctgcgc gccgtcgacc
     5521 gccgccggct gaacgaggcg ctcgccgcgc tgccggcgcc cttccgcgag gcgatcgtgc
     5581 tgcgcgagct cgaggacctc gcgtacaagg acatcgcgcg catcgccgag atcccgatcg
     5641 gcaccgtgat gtcgcgcctc gcgcgcgggc ggcgcctgct cgccgattcg ctgcgcgcga
     5701 tcgagaacgc cgccggtccg aacgcggcgc cgaaggccgc ccgatgaacc gcgacgaagc
     5761 cgcgcgcctg ctcgacgcct acgccgacgg cgagctcgat ccggccgcga gcctggagct
     5821 cgaggcgctg ctcgcgaggg acgcgggctt gcgcgccgcg tgcgagcgca tccgcgcggc
     5881 gtcggcggcg atccgcgcgc aggccgacta ccaccgcgcg ccggcggagc ttgcggcgcg
     5941 cctgcgcgtg tcgatcccgg cgccgcccga gggcgctcgc gccgcggggg gactccggcg
     6001 tcgccgctgg ctcgcgcccg ccgccgcgtt cgccgccgcc gcggcgctga cgtggacgct
     6061 cgcgtggacg acgctgcgcc cggcagcgga ggagcgcctc gccggcgacg cggtcgcgag
     6121 ccacgtgcgc gccacgctcg ccgggcggct cgtcgacgtc gaatcctccg accagcacaa
     6181 cgtgaagccg tggctgagcg cgcggctgcc gtactcgccg cccgtgtccg acttcgccgc
     6241 cgagggcttc gcgctgcgcg gcgcgcgcgt cgacgtgctc gacggccacc cggttgccgt
     6301 gctcggctat cagcggcgcc ggcacacgat cgacgtcttc gtgcgtccgg gagcgggcag
     6361 gagatcaggc ggctacgagc gcgacggctt caacgtcgag cgcgccgccg cggacggcat
     6421 ggagttctgg atcgtgtcgg acctgaaccg cgaggagctg gcgcagctcg cgcgcctgct
     6481 cagcgcgcgc tcgccgggtt gagcagcgcg cgcgcggcgt cgatcacctc gcccgcggtc
     6541 aggcccacgg ggccggcgcc cgcggcgacg aggcggtcgg ccgcggcgcc gtgcaggtac
     6601 acgccggcga ccgcggcgtc ctcgggagac gcgccctgcg cgagcagcgc ggcgatgatc
     6661 ccggtcagca cgtcgcccat tcccgcggaa gccatgcccg gattccccgt cgggttgatc
     6721 caccagcttc cgccgggcgc ggcgatcacg gtgccgtttc ctttcagcgc cacgagcgcc
     6781 gagtagcgct tcgcgatcgc cttcgccgcg gcgacgcgat cggactgcac ctcgtgcgtc
     6841 tcgcgcccga ggaggcggcc ggcctcggcg gggtgcggcg tcatgatggt cggcgcgccg
     6901 cgcgcggcga ccttcttcgc gagccgggcg ctcgccgcga cgaggttgag gccgtcggcg
     6961 tcgagaacca gcggcagctt cgcgccgatc gcggcatcga gcgccttctt cgcggcctcg
     7021 gagcggccga ggccgggtcc ggcggcgagc acggtcagca ggttcttcgc gaacaggtcc
     7081 ttcgccgcgc gcagcatcag ctcgggctgc gccggatcga ccgccggcgg cgcgctgccg
     7141 acgaagccga cgaagacccg cccggcgccg agcagcaacg cggcgcggcc ggcgagcagc
     7201 gccgcgccgg ccat
//
LOCUS       DATDMW010000274         2151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1951303, whole genome shotgun sequence.
ACCESSION   DATDMW010000274 DATDMW010000000
VERSION     DATDMW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951303"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="VI319_11880"
     CDS             <1..1681
                     /locus_tag="VI319_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY6864586.1"
                     /translation="STQALAARLLTKAASLKEKFRASTVGEDGWGPLLDVDPAETTGK
                     IADATILFSDIRNFTTYSERLTAGEIAEFLNAYLQQACAPVLACGGRIMKFLGDGVMA
                     LFEPIAGAANDSHAKRAIRASLAMQLASRGFRDWIRQRHPSRGLPDFAIGVGLHTGEV
                     LMCVVGTAGQGEFTVIGDTVNVASRLEGQTKELGWAIVASDRTIASAGAGVSIGGQQP
                     VQLRGRSAPTTVHEVIGVEADIATDTPVELPADMRDAMAANAQIAAEAAKEALGITLR
                     QVITPHSDTPLSVRGYKVLSKIGQGGSSSVHLAERESDRRQVVLKILNLSLGSYDALL
                     QRFIQEFDIISAIDHPNVVKIYDRGFSEDLAYIAMEYFPGGTLSDIILRGLTPRQALS
                     LLAQAAAGLREIHNHGIVHRDVKPANLMARADGTIALVDFGIAKRLGQNAGSTLHGEV
                     FGTPYYISPEQTEARPVTARSDLYSLGVIFHEMLTRRRPFEADGVAEVLALHTRAPRP
                     KLPQQLAEYQDLLDRMLAVDPERRYPSADALLHAIDEVWTRQAVRVMQSGN"
     gene            complement(1691..>2151)
                     /gene="dapB"
                     /locus_tag="VI319_11885"
     CDS             complement(1691..>2151)
                     /gene="dapB"
                     /locus_tag="VI319_11885"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY6864587.1"
                     /translation="RIAIVLAPNMAVGVNVLFKLAETAAKALGDAYDVEIIEAHHRHK
                     VDAPSGTAIRIGEIVAAALGRDLPSSAAHGRRGNTGERETKTIGFHAIRGGDIVGEHT
                     VMFAGEGERVELVVRSTSRATYALGALRACRFLEGRGPGLYDMQDVLGLR"
BASE COUNT          341 a          752 c          744 g          314 t
ORIGIN      
        1 gtcgacgcag gcgttggccg cgcggctgct tacgaaggcg gcgagcctca aggagaagtt
       61 ccgcgcgagc accgtgggcg aggacggctg ggggccgctg ctcgacgtcg accccgccga
      121 gacgaccggc aagatcgccg atgcgacgat cctgttctcg gacatccgga acttcacgac
      181 gtactcggag cgtctcacgg ccggggagat cgccgagttc ctgaacgcct atctgcagca
      241 ggcgtgcgct ccggtcctcg cctgcggcgg acgcatcatg aagtttctgg gcgacggggt
      301 catggccctg ttcgagccga tcgccggcgc ggccaacgat tcgcacgcga agcgcgcgat
      361 ccgcgcgagc ctcgccatgc agctcgcttc acgcgggttc cgggactgga tccggcagcg
      421 ccacccgtcg cgcgggctgc ccgacttcgc gatcggcgtc gggctgcaca caggcgaggt
      481 gctgatgtgc gtggtcggca ctgcggggca aggcgagttc accgtcatcg gcgacacggt
      541 caacgtcgcc tcgcgcctcg aaggacagac caaggagctc gggtgggcga tcgtggcgag
      601 cgacaggacc atcgcgtcgg ccggcgccgg cgtcagcatc ggcggccagc agcccgtgca
      661 actgcgcggg cgcagcgcgc cgaccacggt ccacgaggtg atcggcgtcg aggccgacat
      721 cgccaccgac acgcccgtag agctgccggc ggacatgcgc gacgccatgg cggcgaacgc
      781 gcagatcgcg gcggaagcgg ccaaggaggc gctcggcatc accctgcgcc aggtcatcac
      841 gccgcattcc gacacgccgc tgtcggtgcg cggctacaag gtgctgtcca agatcgggca
      901 aggtggctcg tcgagcgtcc acctcgccga gcgcgagagc gaccgccggc aggtcgtgct
      961 caagatcctg aacctgtccc tgggcagcta tgacgcactg ctgcagcgct tcatccagga
     1021 gttcgacatc atctcggcga tcgaccatcc gaacgtcgtc aagatctacg accgcggctt
     1081 cagcgaagac ctcgcgtata tcgcgatgga atattttccg ggcgggacgc tgtccgacat
     1141 catcctgcgc gggctgacgc cgcggcaggc gctgtcgctg cttgcgcagg cggcggccgg
     1201 cctgcgcgag atccacaatc acggcatcgt ccaccgggac gtgaagccgg cgaacctcat
     1261 ggcgcgggcc gacgggacca tcgcgctggt cgatttcggc atcgcgaagc gcctcgggca
     1321 gaacgccggc agcacgctgc acggggaagt attcgggacg ccctattaca tctcgcccga
     1381 gcagaccgag gcgcgccccg taaccgcgcg ctcggacctc tactcgctcg gtgtgatctt
     1441 ccacgaaatg ctgacgcgcc gcaggccgtt cgaggccgac ggcgtcgccg aggtgcttgc
     1501 gctgcacacg cgcgcgccgc ggccgaagct gccgcaacag ctcgccgagt accaggatct
     1561 cctcgaccgc atgctcgcgg tcgacccgga gcgccgctac ccgagcgccg acgcgctgct
     1621 ccacgcgatc gacgaggtct ggactcgcca ggccgtgcgg gtgatgcagt cggggaattg
     1681 aggcgggcgc tcagcgcagc ccgagcacgt cctgcatgtc gtagagaccc gggcctcggc
     1741 cctcgaggaa gcggcacgcg cgcagcgcgc cgagcgcata ggtcgcgcgg ctcgtggagc
     1801 ggacgaccag ctcgacgcgc tcgccctccc cggcgaacat caccgtatgc tctccgacga
     1861 tgtcgccgcc gcgaatcgcg tggaatccga tcgtcttcgt ctcgcgctcg ccggtgttgc
     1921 cgcgccggcc gtgcgcggcg ctgctcggga ggtcgcggcc cagagccgcg gcgacgatct
     1981 cgccgatgcg gatcgcggtg cccgaaggcg catccacctt gtgccggtgg tgcgcctcga
     2041 tgatctcgac gtcgtaggcg tcaccgagcg ccttggccgc cgtctccgcg agcttgaaca
     2101 ggacgttcac gccgaccgcc atgttgggcg caagcacgat tgcgatccgc t
//
LOCUS       DATDMW010000275        13102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1453746, whole genome shotgun sequence.
ACCESSION   DATDMW010000275 DATDMW010000000
VERSION     DATDMW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453746"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VI319_11890"
     CDS             complement(<1..1044)
                     /locus_tag="VI319_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004539723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864588.1"
                     /translation="MRPALRLPLLAAGFVALFAGVGAGLARLGWTLPGASLAGVHGPL
                     MICGFFATVISLERAVALGKRWPYLAPIASALGALALVSAQPLAAQLLFLLAGAALVA
                     ACVSIYTRQPALHSLVPVLGAACLTAGGALWLAGPGVAAAVPWWIAFLVLTIAGERLE
                     LSRLLPRPPAAARVFALVVAAIVASLAVSLALPLAGRAALGAATLALAAWLVRYDVAR
                     RTLRGRELPRFVAVCLLSGYAWLAAGAVFLACSPWLPGFARDAGLHAILLGFVFSMVF
                     GHAPIIFPAVTGAAMAYHWSFYVPLALLQASLAVRVAGDFGVLGAWRAAGGAGSAAAL
                     ALFVAGTAAAVARG"
     gene            complement(1061..1366)
                     /locus_tag="VI319_11895"
     CDS             complement(1061..1366)
                     /locus_tag="VI319_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEY6864589.1"
                     /translation="MIGEDEVRDALRDVVDPEVGMNIVDLGLVYGVETSDERIAVRMT
                     MTSPGCPMSETILESARAALQAAAPSASIELDLVWDPPWTPDMMSGIARGHFGWPER"
     gene            complement(1366..2079)
                     /locus_tag="VI319_11900"
     CDS             complement(1366..2079)
                     /locus_tag="VI319_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864590.1"
                     /translation="MNETLVEARGIHSYYGASHVLHGVDFSVRRGEAVGLMGRNGMGK
                     TTLLRTILGLVRPKAGAVKVKGRDMTRAAPHAIARAGLAYVPEGRGIFPNLSVWENLM
                     MAARPGADGRREWTLSRVLESFPRLAERLEHGGAQLSGGEQQMLAIGRALMTTPDLLV
                     LDEATEGIDPKIAHEIWAIIRHLKDEGIATVIVDKNFAAVSAVADRAVILVKGKAVFS
                     GTTAELRAQPELHVRHLGV"
     gene            complement(2081..3562)
                     /locus_tag="VI319_11905"
     CDS             complement(2081..3562)
                     /locus_tag="VI319_11905"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY6864591.1"
                     /translation="MGRMGDVDDRVRIEHRIPYEDLREWLVQVEKLGELRRVNGATWQ
                     EDIGMAAELVQHDENAPAVLFDEVPGCRKGQRVLTNFFGGRRKNMTLGFPLEYSKLEL
                     SQAFLEHYMRDLKPIPHQFVETGPVMENEITGDAIDITKFPVPLWHDADGGRYVGTGS
                     YNITIDPDERWINVGTYRVMIHDKNQLGFYQSPGKHGRIHRDKYQARGEPMPVVIVIG
                     GDPLQFLIACSEMPYGVCELDIAGALRGKPVKVVKGKLTGLPIPANCEMAIEGFVQPG
                     KRLPEGPFGEWTGYYASDVREEPVLDIKAIYHRNDPIILGCPPQRPPEEQARYRAVTR
                     SALIRRYIQQAGVPDVTAVWAHEVGGSRLLLGVSIKQRYPGHVKQAGHVAAMCHAGAY
                     AGKYVVVTDEDIDVSNLEDLMWAVCTRSDPATSIDIIHNTWSTPLDPSIPPEKKEKGD
                     NTNSRAIIDACRPFHWRDKYPKVNMPTAEKARVAREKFGYLLK"
     gene            3713..4306
                     /locus_tag="VI319_11910"
     CDS             3713..4306
                     /locus_tag="VI319_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY6864592.1"
                     /translation="MRRIIVGITGATGAVFGVRLLEALRAAGVESHLVLSKWGQQTVE
                     HETGLSLAQLRELAAEVHGTGNMAATVSSGSFVTEGMIVAPCSMRTLAAIASGVGENL
                     VHRAADVVLKERRKLVLLVRETPLSDIHLENMLRVSRAGAVILPPMPAFYNRPRTIDD
                     LVDHIVARALDQFGIPAEFAKRWDGEMSRPAAVRKLG"
     gene            4333..5904
                     /locus_tag="VI319_11915"
     CDS             4333..5904
                     /locus_tag="VI319_11915"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY6864593.1"
                     /translation="MNEPAKRAAVKRDVTSLRATLEWLRAEGELIETDREVDPDLEIT
                     GLQKHFDGSIPILFNRVKGKPHARAITNLFGSIDVVDKMFGFDGPQDRTRKIAHAINH
                     PLKPVVIDGREAPCQEHVITKDLDVNRWITAIRHTALEPELTIGSGIRCIVGDYFDGG
                     SDLGYNRMNFRWGNVGTFQISPGSHMWQVYTKHYDDDQPIPLTMCFGVPPACTLLAGA
                     GFDYVILPKGCDEIGIAGAVQGSPVRLVKARTVDAWALADCEYVLEGTIHPRDKRYET
                     AESEEKGVQGKTFFHPEWAGYMGKAYKAPTFHVTAITMRKPESKPIIFPLGVHTADDS
                     NIDTTVREAAIFELCNRLQPGIIQDVHIPYCMTDWGGCIIQVRKRNKIEEGWQRNFLS
                     AILSCSQGMRLAIAVSEDVDIYSMDDIMWCLTTRVNPKTDILNPIPGGRGQTFMPAER
                     MTAGDKQWTASNTLFEGGMGIDATVPYGYEQDFHRPVYPVDRVQPERFFSAAQIERMR
                     SRMEGWVLSLARTGR"
     gene            5925..7037
                     /locus_tag="VI319_11920"
     CDS             5925..7037
                     /locus_tag="VI319_11920"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY6864594.1"
                     /translation="MLSGVLVLDCTDRLGWLAGRLLADLGADVVKIEAPGAALDSAEW
                     RALNVNKRVLHVDLDGAAGRAELDPLAAGADILLATFTPGTARAVLFDRERLLKINPR
                     LIVVAITPFGLDGPKAGWLASDLEIMAAGGAMSLAGEPGGTPLRVTAPQSYGWAGSQA
                     AVGALTALAARARTGRGQGVDVSAQAATVVALSHAPAFVDVDGTSPTRAGQFMTGRSV
                     KGARYRVFWRCADGWLNFILYGGVAGRRTNAQLVDWMRARGAPLGALEAVDFERWDPT
                     LADQAEVDALEAPIAAFFATVAKRDFLDEAHRREMLGYPVFDVADIAADPQLAARGFW
                     QDIAAADGVERHCGCFAVVDGRRPELVPLSPETESA"
     gene            7034..8248
                     /locus_tag="VI319_11925"
     CDS             7034..8248
                     /locus_tag="VI319_11925"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY6864595.1"
                     /translation="MSAQALDGLRVVEFGAYAAGPHIGKMLANFGATVVHVESLARPD
                     GFRMQYPPYKDRVPGVDRGGCFAYFNDSKLGVTLDLKNAEGRAIAKRLARWADVVIEN
                     MRPGVIDRLGLGHDALRADHPDLVMLSTCNMGQTGPRADTPGFGSQLTALAGVCGLTG
                     DAEGPPTVLYGPYIDFIASTLGAVAVLAALERRRRTGRGAYIDVSQYEAGLMFVAGPL
                     LEFRRSGRIARRAGNDDPEAAPHGAYRCAGESWIALSCWSRAEFAALGRAVGRPWWGL
                     DPRYATDDGRRGAAADIDRALGAWLRTRDAPAAAETLQRAGVHAHPVESIADLFTDPQ
                     LAARGHWQRRTHPVIGEHSYCGPVFRLSETPGGVTSAAPCLGADNDRVFRGILGIDEA
                     RYAELRTAGAFA"
     gene            8245..8724
                     /locus_tag="VI319_11930"
     CDS             8245..8724
                     /locus_tag="VI319_11930"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY6864596.1"
                     /translation="MSDVIDDTAVRAEIRAFLHANHVMSLATIVDGAPHACSLMYAAV
                     DFSLYWTSEPRTRHSQVLDVEARVAATIAPDYGDFRAIRGVQIAGRARRLRDAADAEL
                     ARKLMSRRFVFLHEIDLLPEPLRRAIVRADYYRLDPETITWIDNTHGFGNRHVLRMT"
     gene            complement(8743..9507)
                     /locus_tag="VI319_11935"
     CDS             complement(8743..9507)
                     /locus_tag="VI319_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864597.1"
                     /translation="MSGPLLSVDRLVAGYGAAVVLDEVSLELAPHSCLALLGRNGVGK
                     STLIATLMGLTRVRRGAVVFDGMDITGWRPHRRARAGMGWVPQERDVFVELTVTENLT
                     VAARPGRWTLARVLELFPRLAERRRNLGGQLSGGEQQMLAIGRALTLNPKLLLLDEPF
                     EGLAPNIVAEVEAAIRAMSAERMAVLLVEQHVDAALRLSQIAVVLEHGRVAHAGPSAE
                     LARDRALLERLVTLPADEPRGARGAAWCGPSATAVS"
     gene            complement(9504..10250)
                     /locus_tag="VI319_11940"
     CDS             complement(9504..10250)
                     /locus_tag="VI319_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864598.1"
                     /translation="MTPALRTEALGRHFGGIRAVNKVSLEFHKGERHAIIGPNGAGKT
                     TLINLLTGVLRPTEGEVFLDGHPVTRLRPDERVKRGLARTFQINSLFPELTVLQSVLL
                     AVCERHGVAVKAWRHLERFAGEIAAAREILEGLGLRGVEGEPVRELAYGQQRLVEIAL
                     ALACRPRVLLLDEPAAGVPRGESEALFQAIAGLPGGVTVVFIEHDMDLVFRFAQRITV
                     LVQGSVLVQGEPREIAADPEVKRVYLGNAA"
     gene            complement(10247..11239)
                     /locus_tag="VI319_11945"
     CDS             complement(10247..11239)
                     /locus_tag="VI319_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6864599.1"
                     /translation="MAYTWSQAARVRAIEAAFWLTLACGFWLWPSYLALWSQIVIAGL
                     FAVSYDLVLGYAGIVSLGHAAYFGVGAYAAGIIARHGWNEPLSGLLFAAICAGAAGLA
                     TGILVVRGRDLTRLMVTTGIGLLLYELANRLAALTGGNDGLIGIEMAPVLGVFRFDMF
                     GRTAYGYSLVVTFVLFLAMRRLVQSPFGLSLRGIRDNPGRMRVLGYPVHRRLILAYTF
                     AAAVAGVAGALLAQTTQFVGTDVLSFNRSADVMVMLIVGGAGMLYGGLVGAAVFMVAH
                     HWLSDLSAVYWQFWLGAFLIAIVLFGRDGVLGAGLRFVQAFVRAFVRFGREGAR"
     gene            complement(11248..12108)
                     /locus_tag="VI319_11950"
     CDS             complement(11248..12108)
                     /locus_tag="VI319_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6864600.1"
                     /translation="MTSLLGVLFDGLAYGMLLFLVSVGLSLTLGLMNFINLAHGAFGM
                     LGGYVCVLALSRWGLPYFVMLPVAFAVSALAGLALEHALYRRLYRRSPLDQVLATIGL
                     TFMAVAAATWFFGPLQQPLQVPAFLSGQASVAGVDLNLYRGFLIALGLILTAALTFAL
                     ERTRFGAQVRAAVDKPRVAEAMGIRVGLVFQVTFAVGCGLAGLGGALGVQVLGLDPLF
                     PLKFIVYFLLVVAVGGTTTLKGPLYAALLLGVIDVAGKYYVPQVGAFIIYAMMVILLV
                     VRPNGLVARS"
     gene            complement(12118..>13102)
                     /locus_tag="VI319_11955"
     CDS             complement(12118..>13102)
                     /locus_tag="VI319_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864601.1"
                     /translation="GRKVVLIVKDTTGTPDVARRVAQELITNDKVDFLAGFGLTPSAL
                     SVAQLATEAKKPMIVMNAATSIITARSPYIARVSFTLPQVTAPLATWAVKNGVTRAYT
                     LVADYGPGIDAETQFKKTFTAAGGQIVGEVRTPMRNPEFAPFIQRIKDAKPQAVFVFL
                     PAGEQAVGFMKGFAERELDKAGIKLIATGDVTEDGVIEAMGDAPLGVITTQHYSVAHQ
                     SPQNALFLKSYAEVAGTRQRANFMAVGAWDGMAAIYEVAKRLNGRIDGDQAMEILKGM
                     KLDSPRGPVVIDPATRDPIETIYVRRVERLKDGKLYNVEFDRFPESKDPTK"
BASE COUNT         2005 a         4671 c         4600 g         1826 t
ORIGIN      
        1 cccgcgcgcg accgcggccg cggtgccggc gacgaacagc gcgagcgccg ccgcgcttcc
       61 ggccccgccc gcggcgcgcc atgccccgag caccccgaag tcgcccgcga cgcggaccgc
      121 gagcgacgcc tgcaggagcg cgagcggcac gtagaagctc cagtgataag ccatggccgc
      181 gccggtgacg gccgggaaga tgatcggcgc gtgcccgaac accatcgaga acacgaaccc
      241 gagcaggatc gcgtgcaggc cggcgtcgcg ggcgaacccg ggaagccagg gactgcacgc
      301 caggaagacc gcgcccgccg cgagccacgc gtatccggag agcaggcaca cggcgacgaa
      361 gcgcggcagc tcgcgtccgc gcagcgtgcg gcgggcgacg tcgtagcgca cgagccacgc
      421 cgccagggcg agcgtcgcgg cgccgagcgc ggcccggccg gcgagcggaa gcgcgagcga
      481 cacggcgagc gacgcgacga tcgcggccac gacgagcgcg aagacgcgcg ccgcggcggg
      541 cggccgcggc agcagccggg agagctcgag gcgctcgccc gcgatcgtga gcacgagaaa
      601 ggcgatccac cacggcacgg ccgcggcgac cccggggccg gcgagccaca gcgcgccgcc
      661 ggcggtcaag cacgccgcgc cgagcaccgg cacgagcgag tgcagggccg gctgccgcgt
      721 gtagatggag acgcaggcgg cgacgagcgc ggctcccgcg aggaggaaca ggagctgcgc
      781 ggcgagcggc tgcgcggaga cgagcgcgag cgcgccgagc gcggacgcga tcggggcgag
      841 atacggccag cgctttccga gcgccaccgc gcgctcgagc gaaatgaccg tcgcgaagaa
      901 gccgcagatc atcagcggcc catgcacccc ggcgagcgat gcgccgggca gggtccagcc
      961 caggcgcgcg agcccggcgc cgacgcccgc gaagagggcg acgaagccgg cggcgagcag
     1021 cggcaatctg agcgccggtc gcaccgcgcg ctccatcgaa tcagcgttcc ggccagccga
     1081 agtggccgcg cgcgatcccg gacatcatgt cgggcgtcca cggcgggtcc cagacgagat
     1141 cgagctcgat gctcgccgac ggcgccgcgg cctgcaatgc cgcgcgcgcc gattccagga
     1201 tggtctcgct catggggcag ccgggtgagg tcatggtcat gcgcacggcg atgcgctcgt
     1261 cgctcgtctc gacgccgtac accagcccga gatcgacgat gttcattccg acctcggggt
     1321 cgacgacgtc gcgcagcgcg tcgcggactt cgtcctcgcc gatcatcaca cccccaggtg
     1381 gcgcacgtgc agctcgggct gcgcgcgcag ctcggccgtc gtccccgaga agaccgcctt
     1441 gcctttcacc aggatgacgg cgcggtccgc caccgcggac accgcggcga agttcttgtc
     1501 gacgatgacg gtggcgatgc cttcgtcctt caggtgccgg atgatggccc agatctcgtg
     1561 ggcgatcttg gggtcgatac cttcggtcgc ctcgtcgagc accagcaggt ccggagtggt
     1621 catgagcgcg cggccgatgg cgagcatctg ctgctcgccg cccgagagct gcgctccgcc
     1681 gtgctcgagg cgctcggcga gccgcgggaa cgactcgagc acgcgcgaga gcgtccactc
     1741 gcgccggccg tccgcgcccg ggcgcgcggc catcatcagg ttctcccaca ccgagagatt
     1801 cgggaagatg ccgcgcccct cgggcacgta ggcgaggccg gcgcgcgcga tcgcgtgcgg
     1861 cgccgcgcgc gtcatatcgc ggcccttcac cttgacggcg cctgccttcg gtctgaccag
     1921 gccgagaatg gtcctcagca gcgtggtctt gcccatgccg ttgcggccca tgagcccgac
     1981 cgcctcgcct cggcgcacgc tgaagtcgac cccgtgcagg acgtggcttg cgccgtagta
     2041 gctgtggatc ccccgggcct cgaccagcgt ctcattcatc ctacttcaaa agatacccga
     2101 acttctcccg tgcgacacgt gccttttccg cggtcggcat gttgaccttc ggatacttgt
     2161 cgcgccagtg gaacggccgg cacgcgtcga tgatcgcgcg cgagttggtg ttgtcgcctt
     2221 tctccttttt ttccgggggg atactcgggt cgagcggcgt gctccaggtg ttgtggatga
     2281 tgtcgatcga ggtggccggg tcggagcgcg tgcacaccgc ccacatcagg tcctcgagat
     2341 tggagacgtc gatgtcctcg tcggtcacga ccacgtactt gccggcgtag gcgccggcgt
     2401 ggcacatggc cgcgacatgg cccgcctgct tcacgtgccc ggggtatcgc tgcttgatcg
     2461 agacgccgag cagcagccgc gagccgccga cctcgtgcgc ccacaccgcg gtgacgtcgg
     2521 ggacccccgc ctgctggatg tagcggcgga tcagcgccga gcgcgtcacc gcgcggtagc
     2581 gcgcctgctc ctcgggcggc cgctgcggcg ggcagccgag gatgatcggg tcgttgcggt
     2641 ggtaaatagc cttgatgtcg aggacgggct cctcgcgcac gtcgctcgcg tagtagccgg
     2701 tccactcgcc gaacggtcct tccggcaggc gcttgcccgg ctgcacgaag ccctcgatcg
     2761 ccatctcgca gttggccggg atcggcaggc ccgtgagctt gcctttcacg accttcaccg
     2821 gcttgccgcg cagcgcgccg gcgatgtcga gctcgcacac gccgtagggc atctcgctgc
     2881 acgcgatcag gaactgcagc ggatcgccgc cgatcacgat gacgaccggc atcggctcgc
     2941 cgcgcgcctg gtacttgtcg cggtggatgc gcccgtgctt gcccggcgac tggtagaagc
     3001 cgagctggtt cttgtcgtgg atcatgacgc ggtaggtgcc gacgttgatc cagcgctcgt
     3061 ccgggtcgat cgtgatgttg tagctgcccg tgccgacgta gcggccgccg tcggcgtcgt
     3121 gccagagcgg gaccgggaac ttcgtgatgt cgatcgcgtc gccggtgatc tcgttctcca
     3181 tgaccgggcc ggtctcgacg aactggtgcg ggatcggctt caaatcgcgc atgtagtgct
     3241 cgaggaacgc ctgcgagagc tcgagcttgg agtattccag cgggaagccg agcgtcatgt
     3301 tcttgcgccg gccgccgaag aaattggtga ggacgcgctg gcccttcctg cagccgggca
     3361 cctcgtcgaa cagcaccgcg ggcgcgttct cgtcgtgctg cacgagctcg gccgccatgc
     3421 cgatgtcttc ctgccaggtc gccccgttga cgcggcgcag ctcgccgagc ttctcgacct
     3481 gcacgagcca ctcgcgcagg tcctcgtacg gaatcctgtg ctcgatgcgc acgcgatcgt
     3541 cgacatcgcc catcctgccc atggtcttct cctcctcggg tcactggaag cgtcaccttc
     3601 gcgcaacgaa ggcggcatcg ggttgacttg catcaaaggg tgcacgggat tcccgcggct
     3661 caatggagtc cggcccgcca atgcgtgtcg ggccggcaaa ggaggaggtc ccttgcgaag
     3721 aatcatcgtc ggcatcaccg gcgccacggg cgccgtgttc ggggtccgcc tgctcgaggc
     3781 gctccgcgcc gcgggggtcg agtcgcacct ggtcctgtcc aagtggggcc agcagacggt
     3841 cgagcacgag accgggctgt cgctggcgca gctgcgcgag ctcgcggccg aggtccacgg
     3901 cacgggcaac atggccgcga ccgtgtcgtc gggctcgttc gtcaccgagg ggatgatcgt
     3961 cgcgccgtgc tcgatgcgca cgctcgccgc gatcgcctcg ggcgtcggcg agaacctggt
     4021 gcaccgcgcg gccgacgtcg tgctgaagga gcggcgcaag ctggtgctgc tcgtgcgcga
     4081 gaccccgctc tcggacatcc atctcgagaa catgctgcgc gtgtcgcgcg ccggcgccgt
     4141 gatcctgccg ccgatgcccg ccttctacaa ccgcccgcgg acgatcgacg acctggtcga
     4201 tcacatcgtc gcgcgggcgc tcgaccagtt cggcatcccc gccgagttcg cgaagcggtg
     4261 ggacggcgag atgagccgcc cggccgcggt gcgcaagctc ggctgaaccc ttaccgaaga
     4321 gaggaaccgc tcatgaacga accggccaag cgagccgccg tcaagcgcga cgtcaccagc
     4381 ctgcgcgcca ccctcgagtg gctgcgcgcc gagggcgagc tgatcgagac cgaccgcgag
     4441 gtcgaccccg acctcgagat caccggcctg cagaagcact tcgacggctc gatcccgatc
     4501 ctgttcaaca gggtcaaagg caagccgcac gcgcgcgcca tcacgaacct gttcggcagc
     4561 atcgacgtcg tcgacaagat gttcggcttc gacgggcccc aggaccgcac gcgcaagatc
     4621 gcgcacgcca tcaaccaccc gctgaagccg gtggtgatcg acggccgcga ggcgccgtgc
     4681 caggagcacg tgatcacgaa ggacctcgac gtcaacaggt ggatcaccgc gatccgccac
     4741 acggcgctcg agcccgagct cacgatcggc tcgggcatcc gctgcatcgt cggcgactac
     4801 ttcgacggcg gatcggacct cggctacaac cgcatgaact tccgctgggg caacgtcggc
     4861 acgttccaga tctcgcccgg ctcgcacatg tggcaggtgt atacgaagca ctacgacgac
     4921 gatcagccga tcccgctcac gatgtgcttc ggcgtgccgc ccgcgtgcac gctgctcgcg
     4981 ggcgccggct tcgactacgt catcctgccc aagggctgcg acgagatcgg catcgccggc
     5041 gcggtgcagg gctcgcccgt gcgcctggtg aaggcgcgta cggtcgacgc gtgggcgctc
     5101 gccgactgcg aatatgtatt ggaaggcacc atccacccgc gcgacaagcg ctacgagacc
     5161 gccgagtcgg aggagaaggg cgtgcagggc aagaccttct tccatcccga gtgggcgggc
     5221 tacatgggca aggcgtacaa ggcgccgacc tttcacgtca ccgcgattac gatgaggaag
     5281 cccgagtcga agccgatcat cttcccgctc ggcgtgcaca ccgccgacga cagcaacatc
     5341 gacacgacgg tgcgcgaggc ggcgatcttc gagctgtgca accggctgca gcccgggatc
     5401 atccaggacg tgcacatccc ctactgcatg accgactggg gcggctgcat catccaggta
     5461 cggaagcgca acaagatcga ggagggctgg cagcgcaatt tcctctcggc gatcctgtcg
     5521 tgctcgcagg ggatgcgcct ggcgatcgcg gtgtccgagg acgtggacat ctactcgatg
     5581 gacgacatca tgtggtgcct caccacgcgc gtgaacccga agaccgacat cctcaacccg
     5641 atcccgggcg ggcgcggcca gaccttcatg cccgccgagc gcatgaccgc cggcgacaag
     5701 cagtggacgg cgtcgaacac gctgttcgag ggcggcatgg ggatcgacgc cacggtgccg
     5761 tacggctacg agcaggactt ccaccggccc gtctacccgg tcgaccgcgt ccagcccgag
     5821 cgcttcttca gcgcggcgca gatcgagcgc atgaggtcgc gcatggaggg ctgggtgctg
     5881 tcgctcgcgc gcaccgggcg ctagagccca tgtaaaggcg cgggatgctc tcgggcgttc
     5941 tcgtcctcga ctgcaccgac cggctgggct ggctcgccgg gcgtctgctc gcggacctcg
     6001 gcgccgacgt cgtgaagatc gaggcgcccg gcgcggcgct cgattcggcg gagtggcgcg
     6061 cgctcaacgt gaacaagcgc gtcctgcacg tcgatctcga cggcgcagcc ggacgcgccg
     6121 agctcgatcc gctcgcggcc ggggccgaca tcctgctcgc gacgttcacg ccggggacgg
     6181 cgcgcgcggt gctgttcgat cgcgagcggc tgctcaagat caacccgcgg ctcatcgtgg
     6241 tcgcgatcac gccgttcggg ctcgacggcc cgaaggccgg ctggctcgcg tccgacctcg
     6301 agatcatggc cgcgggcggc gcgatgtcgc tcgccggcga gccgggcggg acgccgttgc
     6361 gcgtgaccgc gccgcagtcg tacggctggg cgggatcgca ggcggccgtg ggcgcgctga
     6421 ccgcgctcgc ggcgcgcgcg cgcaccgggc gcggacaggg cgtcgacgtc tcggcgcagg
     6481 ctgcgaccgt cgtcgccctc tcgcacgcgc ccgcgttcgt cgacgtcgac ggcacgagcc
     6541 cgactcgcgc gggccagttc atgacggggc gctcggtgaa aggcgcgcgc taccgcgtgt
     6601 tctggcgctg cgccgacggc tggctcaatt tcattctgta cggcggcgtc gccgggcggc
     6661 gcacgaacgc gcagctcgtc gactggatgc gcgcgcgggg cgcgccgctc ggcgcgctcg
     6721 aagcggtcga cttcgagcgc tgggacccga cgctcgccga ccaggccgag gtggacgcgc
     6781 tcgaggcgcc gatcgcggcg ttcttcgcga ccgtcgccaa gcgcgatttc ctcgacgagg
     6841 cgcaccggcg cgagatgctc ggctacccgg tgttcgacgt cgccgatatc gcggccgatc
     6901 cgcagctcgc ggcgcgcggc ttctggcagg acatcgcggc cgccgacggc gtcgagcgcc
     6961 actgcggctg cttcgccgtg gtcgacgggc gccggccgga gctcgttccg ctctctcccg
     7021 agacggagag cgcatgagcg cgcaggcgct cgacggcctc cgcgtggtgg agttcggcgc
     7081 gtacgcagcc gggccgcaca tcggcaagat gctggccaat ttcggcgcga cggtggtgca
     7141 cgtcgagtcg ctcgctcgcc ccgacggctt ccgcatgcag tacccgccgt acaaggaccg
     7201 cgtgccgggc gtcgaccgtg gcggctgctt cgcctacttc aacgactcca agctcggtgt
     7261 cacgctcgac ctgaagaacg ccgagggccg cgcgatcgcg aagcggctcg cgcgctgggc
     7321 cgatgtcgtg atcgagaaca tgcgccccgg cgtgatcgac cgtctcgggc tcgggcacga
     7381 cgcgctgcgc gccgatcacc cggatctggt catgctctcg acctgcaaca tggggcagac
     7441 cggcccgcgc gccgacacgc cggggttcgg ctcgcagctc accgcgctcg ccggcgtctg
     7501 cgggctcacc ggcgacgccg agggcccgcc gaccgtgctc tacggcccct atatcgactt
     7561 catcgcgtcg acgctgggcg cggtcgcggt gctcgccgcg ctcgagcgcc ggcgccgcac
     7621 gggacgcggc gcctacatcg acgtctcgca gtacgaggcg ggcctgatgt tcgtcgccgg
     7681 gccgctgctc gagttccggc gcagcggacg catcgcgcgg cgcgccggca acgacgatcc
     7741 cgaggccgcg ccgcacggcg cctaccgctg cgcgggagag agctggatcg cgctctcgtg
     7801 ctggtcgcgc gcggagttcg ccgcgctcgg gcgcgcggtc ggccgtccgt ggtggggcct
     7861 cgacccgcgc tacgcgacgg acgacggtcg gcgcggcgcc gccgccgaca tcgaccgcgc
     7921 tctgggcgcg tggctgcgca cgcgcgatgc gcccgccgcg gccgagacgc tgcagcgcgc
     7981 cggcgtgcac gcgcatcccg tcgagtcgat cgccgacctc ttcaccgatc cgcagctcgc
     8041 cgcgcgcggg cactggcagc gccggacgca tcccgtgatc ggcgagcatt cgtactgcgg
     8101 tccggtgttc cggctgtccg agacgccggg cggcgtgacc tcggccgcgc cctgcctcgg
     8161 cgcggacaac gaccgcgtgt tccgcggaat cctggggatc gacgaggcgc gctacgccga
     8221 gctgcgcacc gcgggagcgt tcgcgtgagc gacgtgatcg acgacacggc ggttcgcgcc
     8281 gaaatccgcg cgttcctcca tgcgaatcac gtgatgtcgc tggcgacgat cgtcgacggc
     8341 gccccgcacg cgtgctcgct catgtacgcc gcggtcgatt tctcgctgta ctggacatcg
     8401 gagccgcgca cgcggcattc acaggtgctc gacgtcgagg cgcgcgtcgc cgcgacgatc
     8461 gccccggact acggcgactt ccgcgcgatc cgcggcgtgc agatcgccgg ccgggcgcga
     8521 cgcctccgcg acgcggccga cgccgagctc gcgcgcaagc tgatgtcgcg ccgcttcgtg
     8581 ttcctgcatg agatcgacct gctgcccgag ccgctgcgcc gcgcgatcgt gcgtgccgac
     8641 tactatcggt tggatcccga gacgatcacc tggatcgaca acacgcacgg cttcggcaat
     8701 cggcacgtgc tgcgcatgac gtgacgcagg gactccgcgt ttctaggaca cggcggttgc
     8761 ggatggaccg caccaggccg cgccgcgagc gccgcgcggc tcgtcggccg gcagcgtgac
     8821 gagccgctcg agcaatgcgc ggtcgcgcgc gagctcggcg ctcgggcctg cgtgcgcgac
     8881 cctgccatgc tcgagcacga ccgcaatctg cgacagccgc agggccgcgt cgacgtgctg
     8941 ctcgaccagc agcaccgcca tgcgctccgc gctcatcgcg cggatcgcgg cctcgacctc
     9001 ggcgacgatg ttgggcgcga ggccctcgaa cggctcgtcg agcagcagca gcttcgggtt
     9061 cagcgtcagc gcccggccga tggcgagcat ctgctgctcg ccgcccgaga gctgtccgcc
     9121 gaggttgcgg cgacgctcgg cgagccgcgg gaacagctcg agcacgcgcg cgagcgtcca
     9181 ccggccgggg cgcgcggcca ccgtcaggtt ctcggtcacc gtcagctcga cgaagacgtc
     9241 gcgctcctgc ggcacccagc ccatgccggc gcgcgcgcgc cggtgcgggc gccagccggt
     9301 gatgtccatg ccgtcgaaga cgacggcgcc gcggcgcacg cgcgtgaggc ccatgagcgt
     9361 ggcgatcagg gtgctcttgc cgacgccgtt gcgtccgagc agcgcgaggc acgaatgcgg
     9421 cgcgagctcg agcgatacct cgtccaggac gaccgcggcg ccgtagcccg cgaccaggcg
     9481 gtccaccgag agcagcgggc cgctcacgcc gcgttcccca ggtagacgcg cttcacctcg
     9541 ggatcggccg cgatctcgcg cggctcgccc tgcaccagca ccgagccctg gaccagcacc
     9601 gtaatccgct gcgcgaagcg gaacacgaga tccatgtcgt gctcgatgaa cacgacggtc
     9661 acgccgccgg gcagcccggc gatcgcttgg aacagcgcct cgctctcgcc gcgcggcacg
     9721 cccgccgcgg gctcgtcgag cagcagcacg cgcggccggc acgcgagcgc gagcgcgatc
     9781 tcgaccagcc gctgctgccc gtaggcgagc tcgcgcaccg gctcgccctc gacaccgcgc
     9841 aggcccaggc cctcgaggat ctcgcgcgcc gcggcgatct cgccggcgaa gcgctcgagg
     9901 tgccgccagg ccttgaccgc cacgccgtgg cgctcgcaca cggcgagcag caccgactgc
     9961 agcaccgtca gctccgggaa caggctgttg atctggaagg tgcgcgcgag cccgcgcttc
    10021 acgcgctcgt ccgggcgcag ccgcgtgacc gggtggccgt cgaggaacac ttcgccttcg
    10081 gtgggccgca gcacgcccgt gaggaggttg atgagcgtcg tctttcccgc tccgttgggc
    10141 ccgatgatcg cgtggcgctc gcccttgtgg aactcgagcg ataccttgtt cacggcgcgg
    10201 atgccgccga agtgccggcc cagggcctcg gtgcgcagcg ccggggtcat cgcgcgccct
    10261 cgcggccgaa gcgcacgaac gcgcggacga acgcctgtac gaacctgagg ccggcgccga
    10321 gcacgccgtc gcgcccgaac agcacgatcg cgatcaggaa cgcgcccagc cagaactgcc
    10381 agtagacggc ggagagatcg gagagccagt ggtgcgccac catgaagacg gcggcgccca
    10441 ccaggccccc gtagagcatg ccggccccgc cgacgatcag catcaccatg acgtcggccg
    10501 agcggttgaa cgagaggaca tcggtgccga cgaactgcgt ggtctgcgcg agcagcgcgc
    10561 cggcgacgcc ggccaccgcg gccgcgaacg tgtaggcgag gatcaggcgc cggtgcaccg
    10621 gatagcccag cacccgcatg cggcccgggt tgtcgcggat gccgcgcagg ctcaggccga
    10681 acggcgactg caccaggcgc cgcatggcga gaaacagcac gaaggtcacg accaggctgt
    10741 agccgtatgc ggtgcggccg aacatgtcga agcggaacac tcccagcacc ggggccatct
    10801 cgatcccgat cagcccgtcg ttgccgccgg tcagcgcggc caggcggttg gcgagctcgt
    10861 agagcagcaa tccgatgccg gtcgtcacca tgagccgcgt caggtcgcgc ccgcgcacga
    10921 ccaggatgcc ggtcgcgagg cccgcggcgc ccgcgcagat cgcggcgaac agcaggcccg
    10981 agagcggctc gttccagccg tgccgcgcga taatgcccgc ggcgtatgcg ccgaccccga
    11041 aataggccgc gtgcccgagc gagacgatac cggcgtagcc gagcacgaga tcgtaggaca
    11101 cggcgaacag gccggcgatc acgatctggc tccacagcgc gaggtacgag ggccacagcc
    11161 agaacccgca cgcgagcgtc agccagaacg cggcctcgat cgcgcgtacc cttgcggctt
    11221 gcgaccacgt gtacgccatg ggctccctca cgagcgcgcc accaggccgt tcggccggac
    11281 gacgagcagg atgaccatca tcgcgtaaat gatgaaggcg ccgacctgcg gcacgtagta
    11341 cttgcccgcc acgtcgatca cgcccagcag cagcgccgcg tacagcgggc ccttgagcgt
    11401 ggtggtcccg ccgaccgcca ccacgagcag gaagtagacg atgaacttga gcgggaacag
    11461 cggatcgagc ccgagcacct gcaccccgag cgcgccgccc agtcccgcga gcccgcagcc
    11521 caccgcgaac gtcacctgga atacgagccc cacgcggatg cccatcgcct cggcgacgcg
    11581 cggcttgtcc acggcagcgc gcacctgggc gccgaagcgc gtgcgctcga gcgcgaaggt
    11641 cagcgccgcg gtgaggatca ggccgagcgc gatcaggaag ccgcggtaga ggttgaggtc
    11701 gacgccggcg acgctcgcct gtcccgagag gaacgccggc acctgcagcg gctgctgcag
    11761 cggcccgaag aaccaggtcg ccgccgcgac cgccatgaag gtgagcccga tcgtcgcgag
    11821 cacctggtcg agcggactgc ggcggtagag cctgcggtag agggcgtgct cgagcgccag
    11881 tcccgccagc gccgacacgg cgaatgcgac cggcagcatc acgaaatagg gcaggcccca
    11941 gcgcgacagc gcgagcacgc acacgtaccc gcccagcatg ccgaaggcgc cgtgagcgag
    12001 attgatgaag ttcatcagcc cgagcgtgag cgacagcccg acgctgacga ggaacagcag
    12061 catgccgtaa gcgagcccgt cgaacagcac tcccaacagg ctcgtcacgg cgccatccta
    12121 tttcgtgggg tccttcgact ccggaaaccg gtcgaactcg acgttgtaga gcttgccgtc
    12181 cttcaggcgc tcgacgcggc gcacgtagat ggtctcgatc ggatcgcgcg tcgccgggtc
    12241 gatgaccacg gggccgcgcg ggctgtcgag cttcatcccc ttcaggatct ccatcgcctg
    12301 gtcgccgtcg atcctgccgt tcaggcgctt cgccacttcg tagatcgccg ccattccgtc
    12361 ccaggcgccc acggccatga agttggcgcg ctgccgggtg cccgcgacct cggcgtagga
    12421 cttgaggaag agcgcgttct gcgggctctg gtgcgcgacc gagtagtgct gcgtcgtgat
    12481 gacgccgagc ggcgcgtcgc ccatcgcctc gatcacgccg tcctcggtga cgtcgccggt
    12541 ggcgatcagc ttgatcccgg ccttgtcgag ctcgcgctcg gcgaagccct tcatgaagcc
    12601 cacggcctgc tcgcccgccg gcaggaacac gaacaccgcc tgcggcttcg cgtccttgat
    12661 gcgctggatg aagggggcga actcggggtt gcgcatcggg gtgcgcacct cgccgacgat
    12721 ctggccgccg gcggcggtga acgtcttctt gaactgagtc tcggcgtcga tgcccgggcc
    12781 gtagtcggcg accagcgtgt acgcgcgggt gacgccgttc ttcaccgccc aggtcgcgag
    12841 cggcgccgtc acctgcggca gcgtgaacga gacgcgcgcg atgtacggcg agcgcgcggt
    12901 gatgatcgac gtcgccgcgt tcatgacgat catcggcttc ttcgcctcgg tggcgagctg
    12961 cgccaccgag agcgcgctcg gcgtcagccc gaagcccgcg aggaagtcga ccttgtcgtt
    13021 ggtgatcagc tcctgcgcga cgcggcgcgc gacgtcgggc gtgccggtcg tgtccttcac
    13081 gatcagcacc accttgcggc cg
//
LOCUS       DATDMW010000276         3481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2202776, whole genome shotgun sequence.
ACCESSION   DATDMW010000276 DATDMW010000000
VERSION     DATDMW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202776"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            544..1158
                     /locus_tag="VI319_11960"
     CDS             544..1158
                     /locus_tag="VI319_11960"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HEY6864602.1"
                     /translation="MNNKLSRVALGAVLAASLVSVAQADDRHERRERAEHERAEHYRT
                     PHVVFDDRFHHDHYYPRVGYAVTALPPGRFEVRFRGSPFFFHAGVWYRHAGPSFVVVR
                     PPVGIIVPVLPPAYTTVVVANRPYYYANDVYYVQQYDGYAVVDAPLGTAVPVSAVPPA
                     PQAAAPAASAPAGTWYYCDSSRAYYPYVQSCAEGWRPVAATPPR"
     gene            1224..2513
                     /locus_tag="VI319_11965"
     CDS             1224..2513
                     /locus_tag="VI319_11965"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY6864603.1"
                     /translation="MTRTLRPVAVVGGMRIPFARSGTAYAQLGNSDMLSAAMKALVEK
                     YALRRERIGEVAAGAVIKHSRDWNLAREATLACGLDPHTPAYDVQRACGTSLTATVQL
                     AHRIAAGEIESAIAGGADTVSDVPVAHSRGMQKILLASGRGRTLGERLGPWLKFRPGM
                     LKPSVPGVTELRTGLSMGEHCEIMAKEWKIGRREQDELAAASHRKAAAAWKAGFYRDI
                     VAPFGGLTADNNVRPDSTVEKLATLRPVFDRSSTGTLTAGNSTPLTDGASCVLLASDE
                     WARARGLPVQAYITRSAVAAVDYAGLAGPAEGLLMAPTYAVPEMLDRAGIALQDFDIY
                     EIHEAFAAQVLCTLKAWESEDYCRDKLKRKAPLGSIDRAKLNPKGSSVALGHPFAATG
                     TRIIATLAKELEERGKGRGLISICTAGGMGVTAILER"
     gene            2539..3360
                     /locus_tag="VI319_11970"
     CDS             2539..3360
                     /locus_tag="VI319_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864604.1"
                     /translation="MSAPVPLRVSRLVKHYRTRDGGTLEAVRGVSFDVRAGECFGLLG
                     PNGAGKSTTINCITGFFPPTAGEILIAGVDVHREPKKARAVLGVCAQEETLDSDFRVL
                     DQLVRYATFFRVPVEEGRRRALALLERFGLSDKAKEQVEALSGGMKRRLQVARALISR
                     PKVLLLDEPTTGLDPEVRRVLWEILMEERGEGAAILLCTHYMEEAERLCDRVGVMAEG
                     RILDVDAPRALIARHIGEGEVAEEVRPGAIWRRPPNLEDVYLKLTGTRLHALEAA"
BASE COUNT          493 a         1270 c         1216 g          502 t
ORIGIN      
        1 caggcgctcg tcatccattc gagatacgcg gcggccgcct gagcggcgtc gaaggcttcg
       61 gcgaccgcgg gcgcctcgtg caccgccggc ccgactgcgc ccccgtccgc gtcggcccat
      121 tccaggtacg cggctatcca gccgccgata ttcgcgtcca tcgctcctcc ttccggaacg
      181 cggccggctc ccggagcggg agcggttccg agtcccgtat gaattcctcc ctggaacggg
      241 ccattctcgg cgcgtgccgg acccgtgtct gttgcctgaa tcaacgttcc ggaatctttg
      301 cgggatgacg cacgcgcacc gcccggcgga gggggagcct aggccggacg gtcgcgggcg
      361 gacgctacag tcagcgtcaa ggcccttgcg ggcggcgcgc attctggccg gccggggcgc
      421 ccgtgtctgt gctccggatc aaaggcggga cctgctgccg tacaaatcga tacaaaatcg
      481 gacagaatcc cgcccgccag ggcgctagga tggcaccgtg aactcccgaa cggagagact
      541 gccatgaaca acaagctgtc ccgcgtcgcc ctcggcgccg tgctggccgc gtcgctggtc
      601 tcggtggccc aggccgacga ccgccacgag cgccgcgagc gcgccgagca cgagcgcgcc
      661 gagcactacc gcacgccgca cgtggtcttc gacgatcgct tccaccacga ccattactac
      721 ccgcgcgtcg gctacgccgt gacggcgctg ccgccgggac gcttcgaggt tcgcttccgc
      781 ggctcgccgt tcttcttcca cgcgggggtc tggtatcgcc acgccggccc gagcttcgtc
      841 gtggtgcggc cgccggtcgg gatcatcgtc ccggtgctgc cgccggcgta cacgacggtg
      901 gtggtcgcga accgaccgta ttactacgcg aacgacgtct actacgtgca gcagtacgac
      961 ggctacgccg tggtcgacgc gccgctgggc accgcggttc cggtctcggc cgtgccgccg
     1021 gcgccgcagg ccgccgcgcc ggccgcctcg gcgcccgcgg gcacctggta ctactgcgac
     1081 tcgtcccggg cgtactaccc gtacgtgcag tcctgcgccg aaggctggcg gccggtcgcg
     1141 gcgactccgc cgcgctgaaa gaccgccgcc cgctttttcg taggtagtgc attatcctgt
     1201 ctccctcctc gggagtcagg ataatgaccc gcacgctccg tcccgtcgcc gtcgtcggcg
     1261 gcatgcgcat tcccttcgcc cgctcgggca ccgcgtacgc gcagctcggc aactccgaca
     1321 tgctctcggc cgcgatgaag gcgctggtcg agaagtacgc cctgcgccgc gagcgcatcg
     1381 gcgaggtcgc cgccggcgcc gtcatcaagc actcgcgcga ctggaacctc gcgcgcgagg
     1441 cgacgctcgc ctgcgggctc gacccgcaca cgcccgcgta cgacgtgcag cgcgcgtgcg
     1501 gcaccagcct caccgcgacg gtgcagctcg cgcaccgcat cgccgcgggc gagatcgaga
     1561 gcgcgatcgc gggcggcgcc gacaccgtga gcgacgtccc cgtcgcgcac agccgcggca
     1621 tgcagaagat cctgctcgcg agcgggcgcg ggcgcacgct cggcgagcgc ctcgggccgt
     1681 ggctgaagtt ccgccccggg atgctcaagc cttcggtgcc cggcgtcacc gagctgcgca
     1741 ccgggctctc gatgggcgag cactgcgaga tcatggcgaa ggaatggaag atcggccggc
     1801 gtgagcagga cgagctcgcc gccgccagcc accgcaaggc ggccgcggcg tggaaggcgg
     1861 gcttctaccg cgacatcgtc gcgccgttcg gcgggctcac cgccgacaac aacgtgcgtc
     1921 cggactccac ggtcgagaag ctcgcgacgc tccggcccgt gttcgaccgc agctccaccg
     1981 gcacgctcac cgccggcaac agcacgccgc tcaccgacgg cgcctcgtgc gtgctgctcg
     2041 cctccgacga gtgggcgcgc gcgcgcggac tgccggtgca ggcgtacatc acgcggtccg
     2101 ccgtcgccgc ggtcgactac gcgggcctcg ccgggcccgc cgagggcctg ctcatggcgc
     2161 cgacctacgc cgtgcccgag atgctcgacc gcgccggcat cgcgctgcag gacttcgaca
     2221 tctacgagat ccacgaggcg ttcgcggcgc aggtgctgtg cacgctcaag gcgtgggagt
     2281 cggaggacta ctgccgcgac aagctcaagc gcaaggcgcc gctcggatcg atcgaccgcg
     2341 ccaagctcaa ccccaagggc tcgagcgtcg cgctcggcca tcccttcgcc gcgaccggca
     2401 cgcgcatcat cgcgacgctc gcgaaggagc tggaggagcg cggcaagggc cgcgggctga
     2461 tctcgatctg caccgcgggc gggatgggag tgacggcgat cctcgagcgc tgaagcgctc
     2521 ggctagaatc ggaggagcat gtccgctccg gtccccctgc gcgtctcccg gctcgtcaag
     2581 cattaccgca cccgcgacgg cggcacgctc gaggcggtgc gcggcgtgtc gttcgacgtg
     2641 cgcgcgggcg agtgcttcgg gctgctcggt ccgaacggcg ccgggaaaag cacgacgatc
     2701 aactgcatca ccggcttctt tccgccgacc gcaggcgaga tcctgatcgc gggcgtcgac
     2761 gtgcaccgcg agccaaagaa ggcgcgcgcg gtgctcggcg tgtgcgcgca ggaggagacg
     2821 ctcgacagcg acttccgcgt gctcgaccag ctggtgcgct acgcgacctt cttccgcgtc
     2881 ccggtggagg agggccggcg gcgcgcgctc gcgctgctcg agcgcttcgg gctttcggac
     2941 aaggccaagg agcaggtcga ggcgctctcg ggcgggatga agcgccgcct gcaggtcgcg
     3001 cgcgcgctga tctcgcgccc gaaggtgctg ctgctcgacg agccgacgac cggcctcgac
     3061 cccgaggtgc ggcgcgtcct ctgggaaatc ctcatggagg agcgcggcga gggcgcggca
     3121 atcctgctct gcacgcacta catggaggag gccgagcgcc tgtgcgaccg cgtcggcgtg
     3181 atggccgagg ggcgcatcct cgacgtcgac gcgccgcgcg cgctcatcgc gcgccacatc
     3241 ggcgagggcg aggtcgccga ggaggtgcgg ccgggcgcga tctggcggcg gccgccgaac
     3301 ctcgaggacg tctacctcaa gctcaccggc acgcggctgc acgcgctcga ggccgcatga
     3361 gtcccgtcga ctcgctctgg ggcgtgcgct gcgtgtggct gcgctatttc gacgtgttcc
     3421 gcaagagcct gcgctactac ctggtcacga cgttctccga gccggtgctg tacctgctgt
     3481 c
//
LOCUS       DATDMW010000277         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1454326, whole genome shotgun sequence.
ACCESSION   DATDMW010000277 DATDMW010000000
VERSION     DATDMW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454326"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VI319_11975"
     CDS             complement(<1..399)
                     /locus_tag="VI319_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY6864605.1"
                     /translation="MKGVMKSIAALASLVLLASCGRPADPYDIAKMDLKPAPAKVTNE
                     RHQGAFAEGAALSTGAALKPLDPSPVKTVRLDTTHKIIEIAPGVKFSAWTFGDQVPGP
                     TIRARVGDRIRFSMSNRSDEPVPGVRLTAAP"
     gene            complement(523..2019)
                     /locus_tag="VI319_11980"
     CDS             complement(523..2019)
                     /locus_tag="VI319_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY6864606.1"
                     /translation="MVRGRNRVVVVSNRLPFVFHKEPNGGWRIEPGSGGLVSALLPVL
                     RHRGGTWIGWPGTEGRSDELERTLAETSETAGYSLRPVHLTADEVHNFYHGFANEIIW
                     PLFHDLQSLCNFDPTYWKVYCDVNRRFAQAIMDTASHEDFIWVHDYHLMNVGAELRAL
                     RCRAKIGFFLHIPFPPVDLFVKLPWRARLIEALLHFDLVGFQTLRDRRNFVQCVRALN
                     SEVRFQGKGHIMVAHVGKREVRVGAFPISIDYNTFMRQAAAPAIAERAKELHTLLPKR
                     RLVLGIDRLDYTKGIPHRLNAFNTMLKRYPRLRERMSLIQVVVPSRVDIPEYGELKTT
                     IEQLVGRINGEYAKPGGWVPVWYVYGSLTRSELLAYYRAADIALITPLRDGMNLVAKE
                     YCASSIEEDCVLILSEFAGAATQLRNGAMLVNPFDIEEVADTLKRAYDMDIDERQARM
                     RRMKRSIREYDIFWWVDSFMNAAFTRDLSDFPAPSEATEDLAESSLPF"
BASE COUNT          351 a          754 c          822 g          396 t
ORIGIN      
        1 cggcgccgcg gtgagccgca cgccggggac cggctcgtcg ctgcggttgc tcatcgagaa
       61 gcggatccgg tcgccgacgc gcgcgcggat ggtcgggccc gggacctggt cgccgaaagt
      121 ccaggcgctg aacttcacgc ccggagcgat ctcgatgatc ttgtgcgtgg tatcgaggcg
      181 caccgtcttc accggcgacg ggtcgagcgg cttgagggcg gcaccggtgc tgagcgcggc
      241 gccctcggcg aaggcgccct ggtggcgctc gttcgtcacc ttcgccgggg ccggcttcag
      301 gtccatcttc gcgatgtcgt acggatcggc cggcctgccg caggacgcga gcagcaccag
      361 cgacgcaagc gccgcgatcg atttcattac tcctttcatg ggctttcctc ccggtatctt
      421 ggcccctgag cgtagcgacc cgctcgctcc tcaatttgat atggatcaaa aggacggcga
      481 cccccccggg gccggcgaag gacctttgtc gccctcggcg cctcagaacg gcagcgagct
      541 ctccgccagg tcttcggtcg cctcggacgg ggccgggaaa tccgagaggt cgcgcgtgaa
      601 cgcggcgttc atgaacgagt cgacccacca gaagatgtcg tactcgcgga tcgagcgctt
      661 catgcggcgc atgcgcgcct ggcgctcgtc gatgtccatg tcgtaggcgc gcttgagcgt
      721 gtcggcgacc tcttcgatgt cgaacgggtt gacgagcatg gcgccgttgc ggagctgcgt
      781 cgcggcgccc gcgaactcgg acaggatgag cacgcagtcc tcctcgatcg agctcgcgca
      841 gtactccttg gcgacgaggt tcatgccgtc gcgcagcggg gtgatcagcg cgatatcggc
      901 ggcgcggtaa tacgcgagca gctcggagcg cgtcaggctg ccgtacacgt accacaccgg
      961 gacccagccg ccgggcttgg cgtactcccc gttgatgcgc ccgaccagct gctcgatcgt
     1021 cgtcttgagc tcgccgtatt ccgggatgtc gacccggctc ggcacgacga cctggatcag
     1081 cgacatgcgc tcgcgcagcc gtgggtagcg cttcagcatg gtgttgaacg cgttgaggcg
     1141 gtgcgggatg cccttggtgt agtccaggcg gtcgatgccg agcacgagcc tgcgcttggg
     1201 cagcagcgtg tgcagctcct tcgcgcgctc ggcgatcgcg ggcgccgccg cctgccgcat
     1261 gaaggtgttg tagtcgatgc tgatcgggaa cgcgcccacc cggacctcgc gcttgcccac
     1321 gtgcgcgacc atgatgtgcc ccttgccctg gaagcgcacc tcgctgttca gcgcgcgcac
     1381 gcactgcacg aaattgcgcc ggtcgcgcag cgtctggaag ccgaccaggt cgaagtggag
     1441 cagcgcctcg atcagccgcg cccgccacgg cagcttcacg aacaggtcga ccggcggaaa
     1501 ggggatgtgc aggaagaacc cgatcttggc ccggcagcgc agcgcgcgca gctcggcgcc
     1561 gacgttcatc aggtggtagt cgtggaccca gatgaagtcc tcgtgcgacg ccgtgtccat
     1621 gatcgcctgc gcgaagcggc ggttgacgtc gcagtaaacc ttccagtacg tgggatcgaa
     1681 attgcagagc gactgcaggt cgtggaacag cggccagatg atctcgttgg cgaagccgtg
     1741 gtagaagttg tgcacctcgt cggcggtcag gtgcacgggc cgcagcgagt agccggccgt
     1801 ctcgctggtc tcggccagcg tgcgctcgag ctcgtcggag cgcccttcgg tccccggcca
     1861 gccgatccag gtgccgccgc ggtgccgcag cacggggagc agcgcgctga ccagcccgcc
     1921 cgagcccggc tcgatgcgcc agcccccatt gggctccttg tggaagacga acggaaggcg
     1981 attcgatacg acgacgacgc ggtttcgtcc gcggaccatg cgctgggggc ttgtggggat
     2041 gggcgagcgg ccgtcgtggg tccgcatgat ggttgtaggc ttgtgacagc attccggccg
     2101 aaagtttccc ggcggggttt ctttcgtcga aacgggttga ccggcgcctg acgacgcccg
     2161 gcggcgctac tccaccgtca cggacttcgc gaggttcctg ggcttgtcga cgtcgttgcc
     2221 gcgctcgagc gcggcgtggt aggcgagcag ctgcagcggc accgagtgca ggatgggcga
     2281 gagcatgccg gcgtggtcgg gcatgcggat gacctgcacg ccc
//
LOCUS       DATDMW010000278         3936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1205495, whole genome shotgun sequence.
ACCESSION   DATDMW010000278 DATDMW010000000
VERSION     DATDMW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205495"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VI319_11985"
     CDS             <1..200
                     /locus_tag="VI319_11985"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEY6864607.1"
                     /translation="IQMIVVDSGSVGEWREFRRNIVEDYRRAFGEDPWDIVAVGVMSD
                     TDNSHAQARTLYGDITLRPEK"
     gene            complement(204..1811)
                     /locus_tag="VI319_11990"
     CDS             complement(204..1811)
                     /locus_tag="VI319_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6864608.1"
                     /translation="MGELETGREALERELAEARADAADARRDLAEAERRVAQLDAVLD
                     AMPHAVYIGDAYGIWRANAEALHMLGASSLEDLHDSVGDLGAKFRVRRRRDGDAVPPD
                     QLPFSRALAGMPAHLDTWATRLDTGEDVYIGGHSAPVRLEGETIGAVAVNVDLTQRVV
                     IEEQLRAADRRKDDFLAMLAHELRNPLAPIRNAAHILRLDPGGGERVRQAAAIIARQV
                     DHMTDLVDDLLDVSRVTRGLVTLDKQVVDAREVIAAAAEQTAALVQLRRHRVEIRASE
                     PAFVCGDRTRLVQIVTNLLTNAAKFTSEGGLIRVDVSGDERCVAITVADNGSGIRAEL
                     LPHVFEPFTQEERSVDRSQGGLGLGLALVRSLVELHHGDVTVKSEGPGRGAEFTVSLP
                     RYTEPRTPEAPGKAQSRDETRPRGALDVLVVDDNADAAESLAAVLRAEGHHVHVEHRA
                     KAALEYATASPPRVLLLDIGLPDMDGYELARRMRALAATRGAMLVALTGYGQAGDRER
                     SQAAGFDFHLVKPADPARITALLADLA"
     gene            1932..3437
                     /locus_tag="VI319_11995"
     CDS             1932..3437
                     /locus_tag="VI319_11995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6864609.1"
                     /translation="MSLRPDAVVTAADIERGKRALVQDAAWASVCGSLYGGVVLAGFA
                     VALDAGALVIGLLAAIPFAAQAVQLPAIALIEHVRERRRITVTTATLARGLIFGLAIV
                     PWIAAPGRLPLLVSAQLVITVLGSVAGCSFNSWLHQLLPREGLGQFFARRLFFATSLA
                     CIGSLAAGLIVDHWPWGDHLRAYSLTFGLAGIAGFVSSWFLTRVPEPAMTSAGPHESV
                     LAKMRSPFVDSNFRRVLVFMGAWNFASNLSAPFLTVYLMRQLGYSLSTVTTLWVASQV
                     ANALTLYLWGRLSDRLTNKAVLAAALPVYFGCVLGLVFTALPAHRELTLVLLYGVHIV
                     MGAAAGGRALAAGNIGLKLAPQGQGTAYLAGISLVASVSGGLAPILGGALAEWFEAAH
                     LELLVRWGAAARTGEFRVVAFAHWEFLFAISAALGLYAAHALSRVSEGEEHSERVVMQ
                     QFALEAMRTVNQISSIGGLLGNLSAFGRLIERRLVSRRRPGEPERRTGTGG"
     gene            3456..>3936
                     /locus_tag="VI319_12000"
     CDS             3456..>3936
                     /locus_tag="VI319_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEY6864610.1"
                     /translation="MALPTLSLLETRVLGTLAEKQRTVPDTYPLTLNSLVSGCNQKTS
                     RDPVMDASEAEVQAAIDNLKRYSLVIESSGGRVARYAHNVERVLQLSPEALAVLTALM
                     LRGPQTAGELRIACERLHRFESIPAVEAALKDLAGRSLAVELARLPGSRENRWSHLLS
                     "
BASE COUNT          490 a         1411 c         1437 g          598 t
ORIGIN      
        1 agatccaaat gatcgtggtc gacagcggca gcgtcggcga gtggcgcgag ttccggcgca
       61 acatcgtcga ggattaccgc cgcgccttcg gcgaggaccc gtgggacatc gtcgcggtcg
      121 gggtcatgtc cgacaccgac aactcgcacg cgcaggcgcg cacgctctac ggcgacatca
      181 cgctccgccc ggagaagtga tcgtcacgcg agatcggcga gcagcgcggt gatccgcgcc
      241 gggtcggccg gcttgaccag gtggaagtcg aagccggccg cctgcgagcg ctcgcggtct
      301 ccggcctggc cgtagccggt gagcgcgacg agcatcgcgc cgcgcgtggc cgcgagcgcg
      361 cgcatgcgcc gcgccagctc gtagccgtcc atgtccggca ggccgatgtc gagcagcagg
      421 acgcgcggcg gcgaggccgt ggcgtactcg agcgccgcct tcgcgcggtg ctcgacgtgg
      481 acatggtgcc cctcggcgcg aagcaccgcg gcgagcgact cggcggcatc ggcgttgtcg
      541 tcgacgacca gcacgtcgag cgcgcctcgc ggccgggttt cgtcgcgcga ctgcgctttg
      601 cccggtgcct ccggcgtacg aggctccgtg tagcgcggga gcgatacggt gaactcggcg
      661 ccccgtcccg ggccctcgct cttcaccgtg acgtcgccgt gatgcagctc gaccaggctg
      721 cgcaccagcg ccagcccgag gccgagtccg ccctgcgagc ggtcgaccga gcgctcctcc
      781 tgcgtgaatg gctcgaagac gtgcggcagc agctcggcgc ggatgcccga gccgttgtcc
      841 gcgaccgtga tggcgacgca gcgctcgtcg cccgagacgt cgacgcggat gaggccgccc
      901 tcggaggtga acttcgccgc gttggtcagg aggttggtga cgatctgcac caggcgggtg
      961 cggtcgccgc agacgaaggc cggctcggag gcccggatct cgacgcgatg gcgccgcagc
     1021 tgcaccagcg ccgccgtctg ctcggccgcc gcggcgatga cctcgcgcgc gtcgacgacc
     1081 tgcttgtcca gggtgaccag accgcgcgtg acgcgcgaca cgtcgagcag gtcgtcgacg
     1141 agatcggtca tgtgatccac ctgccgcgcg atgatggcgg ccgcctggcg cacgcgctcg
     1201 ccgccgcccg ggtcgagccg caggatgtgg gccgcgttgc ggatcggggc gagcggattg
     1261 cgaagctcgt gcgcgagcat ggccagaaaa tcgtccttgc gccggtcggc ggcgcgcagc
     1321 tgctcctcga tgacgacgcg ctgcgtgaga tcgacgttga cggcgaccgc gccgatcgtc
     1381 tcgccttcga gccgcacggg agccgagtgt ccgccgatgt agacgtcttc gccggtatcg
     1441 agccgcgtcg cccaggtgtc gaggtgcgcc ggcatgccgg cgagcgcgcg cgagaacggc
     1501 agctggtcgg gcgggacggc gtctccgtcg cgccggcggc gcacgcggaa cttcgcgccg
     1561 aggtcgccga cgctgtcgtg caggtcctcg agcgacgacg ccccgagcat atggagcgcc
     1621 tccgcgttcg cgcgccagat gccgtacgcg tcgccgatat agacggcgtg gggcatcgcg
     1681 tcgagcacgg catcgagctg cgccacgcgg cgctcggcct cggcgaggtc ccggcgcgca
     1741 tcggcggcgt ccgcgcgcgc ctccgcgagc tcgcgttcca gcgcctcccg cccggtctcg
     1801 agttccccca taaggggcgg actgcggccg ccgtgccgga gttccgggcc cgtgcgccgc
     1861 ggggttccac cggtctatgc cgggggaccg agccaccccg ccccggggtc cggcatactc
     1921 cgtgtctcgc catgagcctg cgccccgacg ccgtcgtgac cgccgccgac atcgagcgcg
     1981 gaaagcgcgc gctggtccag gacgcggcct gggcgagcgt gtgcggatcg ctctacggcg
     2041 gcgtggtgct cgcgggcttc gcggtcgcgc tcgacgccgg cgcgctcgtg atcgggctgc
     2101 tcgccgcgat cccgttcgcg gcgcaggcgg tgcagctgcc ggcgatcgcg ctgatcgagc
     2161 acgtgcgcga gcggcgccgc atcacggtga cgacggcgac cctcgcgcgc ggcctcatct
     2221 tcgggctcgc gatcgtgccg tggatcgcgg cgccggggcg gctgccgctg ctcgtgtcgg
     2281 cgcagctcgt catcaccgtc ctcggctcgg tcgcgggctg ctcgttcaac tcctggctgc
     2341 accagctgct gccgcgcgaa gggctggggc agttcttcgc ccggcggctg ttcttcgcga
     2401 cctcgctcgc ctgcatcggg tcgctcgccg cgggcctgat cgtcgatcac tggccgtggg
     2461 gcgatcatct gcgggcgtac tcgctgacgt tcgggctcgc gggcatcgcc ggcttcgtca
     2521 gctcctggtt cctgacccgc gtgcccgagc cggcgatgac gagcgcgggg ccgcacgagt
     2581 cggtgctcgc gaagatgcgc tcgcctttcg tcgactccaa cttccgccgc gtgctcgtgt
     2641 tcatgggcgc gtggaacttc gcgtccaacc tctcggcgcc cttcctcacc gtctacctca
     2701 tgcgccagct cggctactcg ctctcgaccg tgacgacgct ctgggtcgcg agccaggtcg
     2761 cgaacgcgct gacgctgtac ctctggggcc gcctctcgga ccggctgacg aacaaggccg
     2821 tgctcgccgc ggcgctgccg gtctatttcg gctgcgtgct ggggctcgtg ttcaccgcgc
     2881 tgcccgcgca ccgcgagctg acgctcgtcc tgctctacgg ggtgcacatc gtcatgggag
     2941 ccgcggcggg cgggagagcg ctcgccgcgg gtaacatcgg actgaagctc gcgccgcagg
     3001 gccagggcac cgcctacctc gccggcatca gcctggtcgc ctcggtctcg ggcggcctcg
     3061 cgccgatcct cggcggcgcg ctcgcggagt ggttcgaggc cgcgcacctc gagctgctcg
     3121 tccgctgggg cgcggccgcg cgcaccggcg agttcagggt cgtcgccttc gcgcactggg
     3181 agttcctgtt tgcgatctcc gccgcgctcg gcctctacgc cgcgcacgcc ctgtcgcgcg
     3241 tgagcgaggg cgaggagcac agcgagcgcg tcgtcatgca gcagttcgcg ctcgaggcga
     3301 tgcggaccgt gaaccagatc tcgtcgatcg gcgggctgct cgggaacctg agcgcgttcg
     3361 ggcgcctgat cgagcggcgc ctcgtatcga gaaggcggcc gggcgagccg gagcggcgga
     3421 cgggaaccgg cggctgagtc cgctatcatg gagtcatggc tcttccgacg ctctcgctgc
     3481 tcgagacgcg cgtcctcggc acgctcgccg agaagcagcg caccgttccc gacacctatc
     3541 cgctgacgct gaacagcctc gtctcgggct gcaaccagaa gacgagccgc gatccggtca
     3601 tggacgcgag cgaggccgag gtgcaggcgg cgatcgacaa cctgaagcgc tacagcctgg
     3661 tgatcgagtc cagcggcggg cgcgtcgcgc gctacgcgca caacgtcgag cgcgtgctgc
     3721 agctttcgcc cgaggcgctc gccgtgctga cggcgctcat gctgcgcggc ccgcagaccg
     3781 cgggagagct ccgcatcgcg tgcgagcggc tccaccgctt cgagagcatc ccggctgtcg
     3841 aagcggcgtt gaaggacctc gctgggcgct cgctcgcggt cgagctcgcg cgcctgcccg
     3901 gatcgcgcga gaaccggtgg tcgcacctgc tctcgg
//
LOCUS       DATDMW010000279         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1081501, whole genome shotgun sequence.
ACCESSION   DATDMW010000279 DATDMW010000000
VERSION     DATDMW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081501"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VI319_12005"
     CDS             complement(<1..983)
                     /locus_tag="VI319_12005"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY6864611.1"
                     /translation="MPALARLDYRVEIDAPEELKAALEKGLNIVRWRLDPDMDEERLK
                     RLVDEAVKETREAAATDGYFSAQVKAEVDSRAEPWVVHINVEPGERTRVGDVEIRFTG
                     PALIDAGEAQSRMRKVRENWVLRTGQPFKQDDWETAKRQALREFSGWRYAAATISASR
                     ASVDPATHSASLFVEIASGPPFRFGELRVSGTKRYSDQLIENLTPVKPGETYDREKVT
                     LYTRRLLESGYFASVQAEIDANPQLADAAPLRVAVIDAPQHHFESGISYNTDVGPRLE
                     ARYTNQDVLGTWWRFDSSLQLDQKIQSIPLELNTPPREGGVWNSYITGWKQ"
     gene            1059..1802
                     /gene="surE"
                     /locus_tag="VI319_12010"
     CDS             1059..1802
                     /gene="surE"
                     /locus_tag="VI319_12010"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY6864612.1"
                     /translation="MKILVSNDDGYFSPGITLLAEALSRLGEVTVVAPERDRSGASNS
                     LTLDRPLVVRRAANGYYSVNGTPTDCVHIAVTGLLDFTPDVIVSGVNLGANMGDDTIY
                     SGTVAAAVEGYLLGIPSLAVSLTSKAGEHYESAIGVALDMVGRLARRPFGEPVLFNIN
                     VPDVPAERLRGLEVTRLGKRHKAEPVIKLTTPRGESAYWIGPAGGAADSGPGTDFHAV
                     ENHRVSVTPLLMDLTHAAQLPRARAWLER"
     gene            1820..>2370
                     /locus_tag="VI319_12015"
     CDS             1820..>2370
                     /locus_tag="VI319_12015"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY6864613.1"
                     /translation="MTSARTRARMVERLRAKGIRDERVLNALGTVPRHLFLEEALASR
                     AYEDDALPIGFGQTISHPYVVARMIEAVLQASPAGKALEVGTGCGYQAAVMAHVFPEV
                     YSIERIKELHERARRNLLALRLANLRLAYGDGYKGLEQAAPFHSIVVAAAAPQVPQAL
                     VGQLAVGGRMILPLTGNSGEQRLV"
BASE COUNT          357 a          818 c          790 g          405 t
ORIGIN      
        1 tgctgcttcc agccggtgat atagctgttc cagacgccgc cctcgcgcgg cggcgtgttg
       61 agctcgagcg ggatgctctg gatcttctgg tcgagctgca gcgagctgtc gaagcgccac
      121 caggtcccga gcacgtcctg gttcgtgtag cgcgcttcaa ggcgcgggcc gacgtcggtg
      181 ttgtagctga tgcccgattc gaagtggtgc tggggcgcgt cgatcaccgc tacgcgtagc
      241 ggcgccgcat cggcgagctg ggggttcgcg tcgatctcgg cctgcacgct cgcgaaatag
      301 cccgattcga ggagccggcg ggtatagagg gtgacctttt cccggtcgta ggtctcgccc
      361 ggcttcaccg gcgtgaggtt ctcgatcagc tggtcgctat agcgcttggt gcccgagacg
      421 cgcagctcgc cgaagcgaaa cggcggtcca cttgcgatct cgacgaagag cgacgcgcta
      481 tgcgtcgccg gatcgaccga ggcgcggctc gcgctgatgg tcgccgccgc gtagcgccag
      541 ccggagaatt cgcgcagcgc ctggcgcttg gccgtttccc agtcgtcctg cttgaagggc
      601 tggcccgtgc gcaagaccca gttctcgcgc accttgcgca tgcgcgactg cgcctcgccg
      661 gcatcgatga gggccggacc ggtgaagcgg atctcgacgt cgccgacgcg cgtgcgctcg
      721 cccggttcga cgttgatatg cactacccat ggttcggcgc gcgagtcgac ctcggctttg
      781 acctgggccg agaaataacc gtcggtcgca gcggcttccc gggtctcctt taccgcttcg
      841 tcgaccaggc gcttgagccg ctcctcatcc atgtcgggat cgaggcgcca gcgcacgatg
      901 ttcaagccct tctcgagcgc agccttcaat tcctcgggcg cgtcgatctc gacgcgatag
      961 tccaagcggg cgagcgcagg catggcgatt agggcgaaaa ggatcagcac caggacacgc
     1021 accgggctta gacagcggac atcggtagaa tcgttcgcgt gaaaatcctc gtctcgaacg
     1081 acgacggcta cttttccccc ggcatcacgc tccttgccga ggcgctgtcc cgcctcggcg
     1141 aagtcacggt cgtcgcaccc gagcgcgacc gctccggcgc ctcgaactcc cttacgctcg
     1201 accggccgct cgtcgtgcgg cgcgccgcca acggctacta ctcggtgaac ggcacgccga
     1261 ccgactgcgt gcacatcgcg gtgaccgggc tcctcgactt cacccccgac gtgatcgtct
     1321 cgggcgtgaa cctgggggcg aacatggggg acgacacgat ctactccggc acggtcgccg
     1381 cagcggtcga gggctacctc ctcggcatcc cgtcgctcgc cgtctcgctc accagcaagg
     1441 cgggcgagca ctacgaatcc gccatcggtg tcgcgctcga catggtaggg cgcctggcac
     1501 ggcgaccgtt cggcgagccg gtgctcttca acatcaacgt tcccgacgtg ccggccgagc
     1561 gcctgcgcgg cctcgaggtg acgcgcctgg gcaagcgcca caaggccgag cccgtgatca
     1621 agctcaccac gccgcgcggc gagagcgcgt actggatcgg gcccgcgggg ggcgcggccg
     1681 actccggccc gggcaccgac ttccacgcgg ttgaaaacca ccgcgtttcg gtgacgccgc
     1741 ttctgatgga tctcactcac gcggcccagc tgccgcgcgc ccgcgcctgg ctcgagcgct
     1801 gaacgccccc ggcattggga tgacctctgc gcgcacgcgc gcgcgcatgg tcgagcgcct
     1861 gcgcgccaag ggcatccgcg acgagcgcgt gctcaatgcg ctcggcaccg tgccgcgcca
     1921 tctctttctc gaggaggccc ttgccagccg cgcctatgag gatgacgcgc tgccgatcgg
     1981 cttcggccag accatttcgc atccctatgt cgtcgcgcgc atgatcgaag ccgtgctgca
     2041 ggcgagtcct gcgggaaagg cgctcgaggt cggcaccgga tgcggctacc aggcggcagt
     2101 gatggcgcac gtatttcctg aggtgtactc gatcgagcgc atcaaggagc tgcacgagcg
     2161 tgcacgccgc aatctcctgg cgctgcgcct cgcgaacctg cgccttgcct acggcgacgg
     2221 ctacaaggga ctcgagcagg cggcgccgtt tcattcgatc gtcgtcgctg cggctgcgcc
     2281 gcaggtcccg caggcgctcg tgggccagct cgcggtcggt ggcagaatga tattgccgct
     2341 cacgggcaac tccggcgagc agcggctcgt
//
LOCUS       DATDMW010000280         5800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1454919, whole genome shotgun sequence.
ACCESSION   DATDMW010000280 DATDMW010000000
VERSION     DATDMW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454919"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VI319_12020"
     CDS             complement(<1..923)
                     /locus_tag="VI319_12020"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY6864614.1"
                     /translation="MIFANALAIFVALVRSQWLSDFVNRLVEIAGALEPPLLVVLAIL
                     YAVSRWLARVSYGVSLVALFAIVIGVTAATFPLQMLLGPDNASSQLAATLAYAAAGTA
                     VLAWYFHLRQRAFSPALAEARLQALQARIRPHFLFNSLNAVLSLIRTDPKRAETAIED
                     LAELFRTVMSDNRRLTVLADEVSLCRQYLSLEQLRLGERLRVEWHLDPAAEAALVPPM
                     LLQPLVENAIYHGIEPGMDPGLVCISAERDGDRLRLQLSNPYHPEHQHRQGNRMALAN
                     IRERLQLHFDVEARLDTRIAGERYEIDIMIP"
     gene            1069..2466
                     /gene="argH"
                     /locus_tag="VI319_12025"
     CDS             1069..2466
                     /gene="argH"
                     /locus_tag="VI319_12025"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY6864615.1"
                     /translation="MAAKPKAKRSTASGRFAEPPSERVARYTASVAFDRRLAAHDIRG
                     SLAHARMLRAAGILSAADLESIERGLSQIAREVSSGDFPWSLDAEDVHLNIEKRLTEL
                     VGDAGKRLHTARSRNDQVAPDLRLWLRDELDACAAAFVALQSALLDQAERHAATIMPG
                     FTHLQVAQPVTFGHHLLAYFEMLERDRSRLADCRKRLNCLPLGAAALAGTSYPIDRER
                     VARELGFDGVCANSLDAVSDRDFAIEFCACAALAMVHLSRLCEEIILWLNPRFGFISL
                     PDRFCTGSSIMPQKKNPDVPELARGKTGRVIGSLTALLVLMKGQPLAYNKDNQEDKEP
                     VFDAADTLRSTLEVFAEMVPGIEVDEEAMRRAAKEGYSTATDLADYLVRKGVAFRDAH
                     EAVGSAVRFAEESGRDLSELSLEELRKFSRSIEKDVFRVLTLEGSVAARSHPGGTAPE
                     QVLAAVRRAREALPR"
     gene            2475..3752
                     /locus_tag="VI319_12030"
     CDS             2475..3752
                     /locus_tag="VI319_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6864616.1"
                     /translation="MERIGKYEIKRKLGEGATSEVYLCHDPFGNRDVAVKVVYEERIK
                     GSSDPAKLSKKLFVAEASLAGKLKHPHICQIYDAVVAPGMSYIVMEYVDGGTLERFCS
                     SARLLPTDKLVEMIFKCTRALEFARRLGVTHRDIKPANILLSGATDIKISDFGSALMA
                     SAEYSQVTGIGSPAYMSPQQIKELALDHRTDIYSLGVVMYQLLTGSLPFNATNNYSMM
                     YQITSVEPQPPSALRRDIPSALDDIVKRAMAKELERRYQTWDEFSFDLAEVFRGSFMP
                     GSVREVADSEKFNTLRVMPFFSDFSDAELWEVMRISNWRNVEAGTVLMRDGDRGDSFC
                     ILAAGEVKVTKRKKLLNVLSPGECFGEMAYLSKTGNERIAEVSTMSDARIITIRTEDL
                     ERASDACRHRFDRAFMAILVERLALANARLIAA"
     gene            3749..4645
                     /locus_tag="VI319_12035"
     CDS             3749..4645
                     /locus_tag="VI319_12035"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEY6864617.1"
                     /translation="MKRGAPAPWQPAVEAAIARASGAEFSMRALRPVAGGCIHRAFVV
                     EGEGGPWFVKTNDAQHADGFAAEADGLSAILATGVRAPRPLCHGADDAHAWIVMEFVE
                     LGGPPDPARLGAQLAKLHAARGDAYGWRRDNWIGATPQRNARSGDWTEFWAKERLAPQ
                     LALARRNGLGDGLARAGERLLGALPRLLAGYLPRASLLHGDLWGGNAGYLADGEPIVF
                     DPAVYYGDPEADLAMTELFGGFPSAFYHAYREIVPLEPGYEWRKNLYNLYHVLNHANL
                     FGGGYAEQAEAMMRRLLAEAGG"
     gene            complement(4642..4959)
                     /gene="grxD"
                     /locus_tag="VI319_12040"
     CDS             complement(4642..4959)
                     /gene="grxD"
                     /locus_tag="VI319_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY6864618.1"
                     /translation="MDVQQRIKEQVTGHPVVLYMKGTPQFPQCGFSAAAVQILNACGA
                     REVFTVDVLQDADIRQGIKAYANWPTIPQLYVRGEFVGGSDIMREMYQSGELQKLLAE
                     EKV"
     gene            complement(5019..>5800)
                     /gene="prmC"
                     /locus_tag="VI319_12045"
     CDS             complement(5019..>5800)
                     /gene="prmC"
                     /locus_tag="VI319_12045"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY6864619.1"
                     /translation="DAAEARLLLAHALGVTRAQLVAHVERALAPADERRFTALVERRA
                     SGEPVAYIVGEREFWGLALRVSPAVLIPRPETELLVERALALLPEAAGHAILDLGTGS
                     GALAIALARERPRARVVASDVSAEALEIARENARTHGTTMRFALGDWLAAVGPGERFE
                     MIVSNPPYVASGDPHLALGDLRFEPRGALDGGGDGLDCIRRIARAARAHLVPGGWLLL
                     EHGYDQAGACRELLADAGYRELQDYRDLAGHPRVCAGRRPD"
BASE COUNT          915 a         2014 c         2013 g          858 t
ORIGIN      
        1 tagggaatca tgatgtcgat ctcatagcgc tcgccggcga tgcgcgtgtc gaggcgcgcc
       61 tcgacgtcga aatgcagctg caggcgctcg cggatattgg cgagcgccat gcggttgccc
      121 tgccgatgct ggtgctcggg atggtacggg ttggacagct gcaggcgcag ccggtcgccg
      181 tcgcgctcgg cgctgatgca caccagcccc gggtccatgc cgggctcgat gccgtgatag
      241 atcgcgttct cgaccagcgg ctgcagcagc atcgggggca cgagcgcggc ctcggccgcg
      301 gggtcgagat gccattcgac ccgcaggcgc tcgccgagcc gcagctgctc gaggctcaga
      361 tattgccggc acagcgaaac ctcgtcggcc agaacggtga gccggcggtt atcggacatg
      421 acggtgcgga acagctcggc gaggtcctcg atagcggtct cggcccgctt gggatccgtg
      481 cggatgagcg agagcaccgc gttcagactg ttgaacagga aatgtggccg gatgcgagcc
      541 tgcagcgcct gcaggcgcgc ctccgcgagc gccggcgaga acgcgcgctg ccgcagatgg
      601 aaataccagg cgaggactgc ggtgcccgcg gccgcgtacg cgagcgtcgc ggcgagctgc
      661 gacgaggcgt tgtcgggccc gagcagcatc tgcaggggaa aggtcgcggc cgtcacgccg
      721 atcacgatcg cgaacagcgc gaccagcgaa accccgtacg agacccgcgc gagccatcga
      781 gagacggcgt acaggatggc caggacgacg agcagcgggg gctcgagcgc acccgcgatc
      841 tcgaccagcc ggttcacgaa gtcggacagc cactgcgagc gcaccagagc gacgaaaatg
      901 gcgagcgcgt tggcgaaaat cacgatgcgc gagatgacgc ccaggttcct gaaatcgggc
      961 agggcgtcgg gcagatcgtt ttgccgtata ctttgagtcc tctgctccga caaatttagc
     1021 tccggcggcg gcgcgcaggg cgctgtccgg gccattttac agataacgat ggcagccaag
     1081 cccaaagcca agcgcagcac cgcgtcgggc cggtttgccg agccgccctc ggagcgggtt
     1141 gcgcgctaca ccgcgtccgt ggcgttcgat cgccggctcg cggcgcacga catccgcggc
     1201 tcgctcgcgc acgcgcgcat gctgcgcgcg gccggcatcc tgtccgccgc ggacctggaa
     1261 agcatcgagc gcggcctgtc gcagatcgcg cgcgaagtgt cctcgggcga cttcccctgg
     1321 tcgctcgacg ccgaggacgt gcatctcaac atcgagaagc ggctgaccga gctcgtcggg
     1381 gacgccggca agcggctgca caccgcgcgc tcgcgcaacg accaggttgc gcccgacctt
     1441 cggctatggc tgcgcgacga gctcgacgcg tgcgccgcgg cgttcgtggc gctgcagtcg
     1501 gcgctgctcg accaggccga gcggcacgcg gcgacgatca tgcccgggtt cacgcacctg
     1561 caggtggcgc agccggtcac gttcgggcat cacctgctcg cgtatttcga gatgctggag
     1621 cgcgatcgct cgcggctcgc ggactgcagg aagcgcctca actgcctgcc cctcggcgcc
     1681 gccgcgctcg ccggcacctc gtacccgatc gaccgcgaac gcgtcgcgcg tgagctcggc
     1741 ttcgacggcg tgtgcgcgaa ttcgctggac gcggtctccg accgggactt cgcgatcgag
     1801 ttctgcgcct gcgcggcgct ggcgatggtg cacctgtcgc ggctgtgcga ggagatcatc
     1861 ctgtggctga acccgcgctt cggcttcatt tcgcttcccg accgcttctg cacgggctcg
     1921 tcgatcatgc cgcagaagaa gaaccccgac gtgcccgagc tcgcgcgcgg caagaccggg
     1981 cgcgtcatcg gcagcctcac ggcgctgctc gtcctcatga agggccagcc tctcgcctac
     2041 aacaaggaca accaggagga caaggagccc gtgttcgacg ccgccgacac gctgcggagc
     2101 accctcgagg tattcgccga gatggtgccc gggatcgagg tcgacgagga ggcgatgcgc
     2161 cgggcggcga aggagggtta ctccaccgcg acagatctcg cggactacct ggtgcgcaag
     2221 ggcgtcgcct tccgcgatgc ccacgaggcg gtcgggagcg ccgtgcgctt cgccgaggaa
     2281 tccgggcgcg acctgtcgga gctctcgctc gaggagctgc gcaagttctc ccgctcgatc
     2341 gagaaagacg tgttccgggt cctgacgctc gaaggctcgg tcgccgcccg ctcgcacccg
     2401 gggggcaccg cccccgagca ggtgctggcc gcggtgcgtc gcgcgcgcga ggcgctcccg
     2461 cgctgacctc accgatggag cgcatcggca agtacgagat caagcgcaag ctcggcgagg
     2521 gcgcgacgag cgaggtctac ctctgccacg acccgttcgg caaccgcgac gtcgcggtga
     2581 aggtggtcta cgaggagcgc atcaagggct cgagcgatcc ggcgaagctc tccaagaagc
     2641 tgttcgtcgc cgaggcgtcc ctcgcgggca agctcaagca cccgcacatc tgccagatct
     2701 acgacgccgt cgtggctccc gggatgagct acatcgtgat ggagtacgtc gacggcggca
     2761 cgctcgagcg cttctgcagc tcggcgcgcc tcctgccgac cgacaagctg gtcgagatga
     2821 tcttcaaatg cacgcgcgcg ctcgagttcg cgcgccgcct cggcgtcacg caccgcgaca
     2881 tcaagcccgc gaacatcctg ctgtcgggcg cgacggacat caagatctcg gatttcggct
     2941 cggcgctgat ggcgagcgcc gagtacagcc aggtcaccgg gatcgggtcg ccggcttaca
     3001 tgtcgccgca gcagatcaag gagctggcgc tcgaccaccg caccgacatc tattcgctcg
     3061 gcgtggtgat gtaccagctg ctcacgggct cgctgccctt caacgcgacc aacaactaca
     3121 gcatgatgta ccagatcacg agcgtcgagc cacagccgcc gtccgcgctg cggcgcgaca
     3181 tcccgagcgc gctcgacgac atcgtcaagc gcgcgatggc caaggagctc gagcgtcgct
     3241 accagacctg ggacgagttc tcgttcgatc tcgccgaggt gttccggggc agcttcatgc
     3301 ccggctcggt ccgcgaggtc gccgacagcg agaagttcaa cacgctgcgc gtcatgccgt
     3361 tcttctcgga cttctccgac gccgagctgt gggaggtgat gcgcatctcg aactggcgca
     3421 acgtcgaggc cggcacggtg ctcatgcgcg acggcgaccg cggcgactcc ttctgcatcc
     3481 tcgcggccgg cgaggtgaaa gtgaccaagc gcaagaagct gctcaacgtg ctctcgcccg
     3541 gtgaatgctt cggcgagatg gcctacctgt ccaagaccgg gaacgagcgc atcgccgagg
     3601 tctcgaccat gagcgacgcc cgcatcatca cgatccgcac cgaggacctg gagcgcgcct
     3661 ccgacgcgtg ccggcaccgc ttcgaccgcg ccttcatggc gatcctggtc gagcgcctgg
     3721 cgctcgccaa cgcccgcctg atcgcggctt gaagcgcggc gcgccggctc cctggcagcc
     3781 ggcggtcgag gcggcgatcg cgcgcgccag tggagcggag ttctcgatgc gtgcgctgcg
     3841 gccggtcgcc ggcggctgca ttcatcgcgc gttcgtcgtc gaaggcgaag ggggcccctg
     3901 gttcgtgaag acgaacgacg cgcagcacgc cgacggcttc gccgccgagg cggacgggct
     3961 gtcggccatc ctcgcgacgg gcgtgcgtgc gccgcggccg ctgtgccacg gcgccgacga
     4021 cgcgcacgcg tggatcgtca tggagttcgt cgagcttggc ggcccgcccg atccggcgcg
     4081 gctcggcgcg cagctcgcga aactccacgc cgcgcgcggc gacgcttacg gctggcgccg
     4141 cgacaactgg atcggcgcca cgccgcagcg caatgcccga agcggcgact ggaccgagtt
     4201 ctgggcgaag gagcgcctcg cgcctcagct cgcgctcgcg cggcgcaacg gcctgggaga
     4261 cgggctggcg cgcgcgggcg agcggctgct cggcgccctg ccgcgccttc tcgccgggta
     4321 tctcccgcgg gcgtcgctgc tgcacggcga cctctggggc ggcaacgcgg gctatctcgc
     4381 ggacggcgag ccgatcgtat tcgatcccgc cgtgtactac ggcgacccgg aagccgacct
     4441 cgcgatgacc gagttgttcg gcggcttccc gtccgcgttc tatcacgcgt accgggagat
     4501 cgtgccgctg gagccgggct acgagtggcg caagaatctc tacaacctct accacgtgct
     4561 caaccacgcc aaccttttcg gcggcgggta cgcggagcag gccgaagcga tgatgcggcg
     4621 gctgctcgcc gaggccggag gctagacctt ctcttcggcg agcagcttct gcagctcgcc
     4681 cgactggtac atctcgcgca tgatgtcgga gccgccgacg aactcgccgc ggacatagag
     4741 ctgggggatc gtcggccagt tcgcgtaggc cttgatacct tggcggatat cagcgtcctg
     4801 cagcacgtct acggtgaaga cctcgcgcgc cccacaggcg ttcaggatct ggacggcggc
     4861 ggcggagaag ccgcactgcg gaaactgcgg cgtacccttc atgtagagca ccaccgggtg
     4921 cccggtcacc tgctccttga tccgctgctg cacgtccatg ccggctcctt caatacctga
     4981 ttgatttact cggattttat cggacagcct tgtgccggtc aatcgggccg acggcctgcg
     5041 cacacgcgag ggtgacccgc gaggtcgcgg tagtcctgca gctcgcggta gcccgcgtcc
     5101 gcgagaagct cccggcacgc ccccgcctga tcgtagccgt gctcgagcag cagccagccg
     5161 ccgggaacca gatgcgctcg cgccgcgcgc gcgatgcgcc ggatgcaatc gagcccgtcg
     5221 ccgccgccgt ccagggcgcc tcgcggctcg aagcgcaggt cgccgagcgc gaggtgcgga
     5281 tcgcccgagg cgacgtacgg cggattcgag acgatcatct cgaagcgctc tcccgggccc
     5341 accgccgcga gccagtcgcc gagcgcgaac ctcatggtcg tgccgtgcgt gcgcgcattt
     5401 tcgcgcgcga tctccagcgc ttcggcggag acgtcgctcg cgaccacgcg cgcccgcgga
     5461 cgctcgcgtg cgagcgcgat cgcgagggct ccgcttcccg tccccaggtc gaggatggcg
     5521 tgcccggccg cctccggcag cagcgcgagc gcgcgctcga cgaggagctc cgtctcgggc
     5581 cgcgggatga gcactgcggg agaaacgcgc agcgcgagcc cccagaactc gcgctcgccg
     5641 acgatgtagg cgaccggctc gcctgacgcg cgccgctcga cgagcgcggt gaagcgccgc
     5701 tcgtccgcgg gcgcgagcgc gcgctcgacg tgcgccacga gctgcgcgcg cgtcacaccg
     5761 agcgcgtgcg cgaggagcag gcgcgcctcg gctgcgtcga
//
LOCUS       DATDMW010000281         8838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1331449, whole genome shotgun sequence.
ACCESSION   DATDMW010000281 DATDMW010000000
VERSION     DATDMW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331449"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VI319_12050"
     CDS             <1..538
                     /locus_tag="VI319_12050"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEY6864620.1"
                     /translation="RTGTPEADQKLLRSLIVFACLMEGLFFYVGFTQILALGRQNKMT
                     GAAEQYQYILRDESMHCNFGIDLINQIKMENPHLWTSEFREEIQGLIRKAVELEYRYA
                     EDTMPRGVLGLNAAMFKEYLRFIANRRCQQIGVEPMFAGATNPFPWMSEMIDLKKERN
                     FFETRVIEYQTGGALSWD"
     gene            567..845
                     /locus_tag="VI319_12055"
     CDS             567..845
                     /locus_tag="VI319_12055"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEY6864621.1"
                     /translation="MHRVTQCKASRDYRLWLRFDDGVEGSVFLGDLLEVGAFTAWRDV
                     EQFCRAAVDAATVVWEAGIRLDPDILYQDLLWAKAGTGFNPRRSGLGG"
     gene            842..1222
                     /locus_tag="VI319_12060"
     CDS             842..1222
                     /locus_tag="VI319_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864622.1"
                     /translation="MKDPRVSGGSRFFITQKEVGHGQEESEEGKEVCEEEEKSSRPSS
                     GREVHQADGCKAQGGGKKESRAQENGRPQARAPRDPDGGAPGAGGYPGAGSIDGGHPR
                     RQDRSEPGCGLAVSDGVPALMGGG"
     gene            1598..2287
                     /locus_tag="VI319_12065"
     CDS             1598..2287
                     /locus_tag="VI319_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY6864623.1"
                     /translation="MNKAELKQSLRTGIRAIATTLHHGLAFIGLAAVIFLVMQSAVGL
                     PENLDRAAAQGPIRFDGDLLADRADDPQVARHRALVNYLSRRYHVASSATEQLVDAAY
                     DAGQQVGLDPLLILAVMAIESRFNPIAESGVGAKGLMQVIPKHHQDKLEAGDEVLDPT
                     TNILLGARILKEYIRRTGSLEAGLQFYNGALADVSSQYAQKVMAEKERLDEALRRVNR
                     PQPQQTARNSA"
     gene            complement(2307..3476)
                     /locus_tag="VI319_12070"
     CDS             complement(2307..3476)
                     /locus_tag="VI319_12070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY6864624.1"
                     /translation="MKREHEPRLAARMASIEPFHVMDVQARAHALEAQGRRIVHMEIG
                     QPDFGAPPQVAEAAVAAIRTRRLGYTASAGLPELREAIARHYRERLGADVPAERIVVT
                     TGASGALLLGLGVLLNPGDEALMPDPCYPCNRHFVRLFDAVPRTVAVDERQSYQLTAA
                     DVRSAWTGATRCVMLASPSNPTGTLIEPDALREIADETRRRGGTVVVDEIYQGLVYGR
                     RPSTALSIADDVFVVASFSKYFNMTGWRLGWMVVPERYAREVEKLSQNVAICPPAPSQ
                     HAALAAFRPDTLAVLEERRAEFERRRDFMVPALRRLGFRIPLVPEGAFYIYAGCERFG
                     DSWDLALDLLERAGVAITPGIDFGSNRPREHVRFAYTRALPDLEEGVAAIARALA"
     gene            3539..5878
                     /locus_tag="VI319_12075"
     CDS             3539..5878
                     /locus_tag="VI319_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY6864625.1"
                     /translation="MIPLPIGAETAMDDSVRKSALDYHRLPRPGKISIVPTKGLVNQR
                     DLALAYTPGVAAACDEIVRDPAQAHALTARGNLIGVVTNGTAVLGLGAIGPLASKPVM
                     EGKSVLFKKFAGIDCFDIELNERDPDKLVDIIAALEPTFGGINLEDIKAPECFLIEKK
                     LRERMKIPVFHDDQHGTAIIVGAAVLNGLRVVGKDIARVKLVCSGAGAAALACLDLLV
                     GLGLSMENVWVSDIKGVVYEGRKEEMDPNKARYARKTSARALADIISDADVFLGLSAG
                     GVLQPEMVKRMADRPLILALANPEPEIQPDIARSARPDALIATGRSDYPNQVNNVLCF
                     PFIFRGALDVGATAINEAMKLAAVRAIADLAMAEQSEVVTAAYGEQNLKFGPDYLIPK
                     PFDPRLIAKIAPAVAKAAMDSGVATRPIEDLEAYKDQLQQFVYTSGLIMKPIFAAAKE
                     GPKRRIIFAEGEDERMLRAAQVVIDEGLAAPVLIGRPAVVERRIERYGLRIAPGKDFE
                     LVNPEYDPRFREYWEEYFKLVERRGVSPQYAQIEMRRHHTLIGAMAIHRGEADGMICG
                     AFGTHELHLRYIEQVIGRRAGVNHYYAMNVVMLPKRTVFICDTYVNLDPTAEQIAEMT
                     VLAAEEVRRFGVAPKIALLSHSSFGTSDAPSARKMREALALLERNAPELDIEGEMHGD
                     AALNESLRLASFPRSRLKGEANVLIMPTLDAANIAFNLLKTAAGDGITIGPILLGAAR
                     PVHILTPSATVRRIVNVTALTVVDVNAAREQMRLFARPG"
     gene            5940..7082
                     /locus_tag="VI319_12080"
     CDS             5940..7082
                     /locus_tag="VI319_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY6864626.1"
                     /translation="MEPTKPKAGLVLTGGGARAAYQVGVLKAVSELMARPERNPFPIL
                     CGTSSGAINAATLAVFANNFGEGVEKLLDVWADCRVHHVYRSDPLAVARSGARWLAAM
                     MLIARSSPVSLLDNTPLAELLARSLDFGRIQQNIDDGHLYAVSITCSGYSSGQSVSFY
                     QGGPGTEAWERTQRVGAAMPIGVEHLMASSALPFIFPAVKINREYFGDGSMRQMAPVS
                     PALHLGSDRVLVIGTGRHVNVEPPRVRSSLYPSLAQVAGHALNSIFLDSLYVDIERLH
                     RINRTISLIPAERLREAGLPLRKIKVLVIQPSQPLERIAVRFLPDLPWTIRFLLRGIG
                     AMNRNGSTLASYLLFEKGFCQALIDLGYEDTMARRPEVLEFLEAAH"
     gene            7122..7517
                     /locus_tag="VI319_12085"
     CDS             7122..7517
                     /locus_tag="VI319_12085"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEY6864627.1"
                     /translation="MSQSPDPFEFMKSLWAPMGLPGVMAPTLDVGEVDRRIAELKSVE
                     NWLNMNLAALRMTIQGLEMQRATLAAMQGGAQAKGSPGGGDAGEAGRRFAEAWLKMLQ
                     QAPSAGSGSTSAGASSSASPGKDKEPGKK"
     gene            7514..8647
                     /locus_tag="VI319_12090"
     CDS             7514..8647
                     /locus_tag="VI319_12090"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HEY6864628.1"
                     /translation="MSLTFRHAHASARRWQDAARGCAETLGSGPRGANLGFLYFSDHY
                     AAHAAELLDYLKEETGVDEWVGTVGIGVIATGTEYLDEPAMSVMLGAFPPDSYRVFSG
                     RQRPPRAGTRTLTGSLAGNFAVVHGDPQTPDIPELIDDMSRKVDSGFLVGGLSSSRTR
                     TVQIANEVLQGGLSGVIFSSDVAIATRLTQGCAPLTRSDTHAPVRHRVTSCERNVIAA
                     LDDRPALDVFREEIGDDLARDLGRAARHVLVGLSVPGSDTGDYLARNVVGIDPRNKLF
                     AIGAPVEQGMSIMFCRRDGEAAREDLVRMLKGLRAELRGVPRGGLYYSCIGRGAQMFG
                     HKSAELEIIRQELGDFPMVGFFCNGEISHDRLYGYTGVLTLFV"
     gene            8674..>8838
                     /locus_tag="VI319_12095"
     CDS             8674..>8838
                     /locus_tag="VI319_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY6864629.1"
                     /translation="MPKAIRIQKQGGPEVMQWEDVQVGDPGPGEARVRHKAVGLNYLD
                     TYQRGGLYKIA"
BASE COUNT         1471 a         3027 c         3051 g         1289 t
ORIGIN      
        1 cagaacggga acccccgaag ccgaccagaa gctgctgcgc agcctgatcg ttttcgcgtg
       61 cctgatggag ggattgttct tctacgtcgg gttcacgcag atcctcgcgc tgggacgcca
      121 gaacaagatg accggcgccg cagagcagta ccagtacatc ctgcgcgacg agtcgatgca
      181 ctgcaatttc ggcatcgacc tgatcaacca gatcaagatg gagaacccgc acttgtggac
      241 ctccgagttc cgcgaggaga tccaggggct gatccgcaag gcggtcgagc tcgagtaccg
      301 ctatgcggag gacacgatgc cgcgcggggt gctgggactg aacgccgcca tgttcaagga
      361 atacctgcgg ttcatcgcca accgccgttg ccagcagatc ggcgtggagc cgatgttcgc
      421 cggcgccacg aaccctttcc cgtggatgag cgagatgatc gacctgaaga aggagcgcaa
      481 cttcttcgag acgcgagtga tcgagtacca gaccggaggt gcgctgagtt gggactagcg
      541 cccggtcgca gcggacggag agcggcatgc atcgcgtaac gcagtgcaag gcctcgcgcg
      601 attaccggct gtggctcagg ttcgacgacg gcgtcgaggg cagcgtgttc ctcggcgatc
      661 tgctggaggt cggcgcgttc accgcgtggc gcgacgtcga gcagttctgc cgggccgccg
      721 tcgacgcggc gaccgtcgta tgggaggcgg ggatccggct ggatcccgat atcctgtacc
      781 aggacctgct ctgggcgaag gcggggacag gattcaatcc gcggaggagc ggcctcggcg
      841 gttgaaggac ccgcgggttt cgggcgggtc gcggtttttc ataactcaga aggaggtcgg
      901 ccatggccaa gaagagagcg aagaaggcaa agaagtctgc gaagaagaag aaaagagcag
      961 caggccgtcg agcggccgcg aggtccatca agcggacggg tgcaaagcgc aaggcggcgg
     1021 taagaaagag tcccgcgcgc aagaaaacgg gcggccgcaa gcgcgcgcgc cgcgcgaccc
     1081 cgatggcggc gccccaggcg ccggcggcta ccccggcgcc ggctccatcg acggcggcca
     1141 cccccggcgc caagaccgct ctgaacccgg ctgcggcctg gccgtttccg acggggtccc
     1201 ggccctgatg ggcggcgggt aggctgcgga cagcccgtga cccgggtccg ggctgcgccg
     1261 cagtcatccg cgccttttgc gacgcagcat gacggcgtca agccgatgat aaataaaggt
     1321 taatccagct tgaccgcgcg gtggggcgcc gatacaatcc gtcccctcac tgtgccaaaa
     1381 aattcacggc cgggaactgt ccgccgggat ttttgactta gtggtgacgg ccggtaagca
     1441 atccggcccg tgggccccgt tccgggcccc ggcggccgtc cccagaacta gaacgagaaa
     1501 tccgccagga gaggactcac ccgcgctttc ggggagcgcg gcgcttcatg caagttccac
     1561 gcgtcctgct ttcgggcagg ccggcaagga gagtgcaatg aacaaagccg aactgaagca
     1621 aagcttgcgc acgggcatcc gggcgatcgc gacgacgctg catcatgggc tcgctttcat
     1681 cgggctggcc gcggtgatct tcctggtcat gcagagcgcc gtcgggctgc ccgagaacct
     1741 ggaccgcgcc gcggcgcagg ggccgatccg attcgacggc gacctgctcg cagaccgcgc
     1801 cgacgacccg caggtcgcca ggcaccgcgc gctggtcaac tatctttcgc gccgctatca
     1861 cgtggcctcc tcggccacgg agcagctggt cgacgcggcg tacgacgccg gccagcaggt
     1921 cggcctcgat ccgctgctga tcctcgccgt gatggcgatc gagtcgcgct tcaatccgat
     1981 cgccgaaagc ggcgtcgggg cgaaggggct gatgcaggtg atccccaagc accaccagga
     2041 caagctcgag gccggcgacg aggtgctcga ccccacgacg aacatcctgc tgggcgcgcg
     2101 catcctgaag gaatacatcc ggcgcaccgg cagtctggag gcgggccttc agttctacaa
     2161 tggcgcgctc gccgacgtat cgagccagta cgcgcagaag gtgatggccg agaaggagcg
     2221 cctcgatgag gcgctcaggc gcgtcaaccg gccgcagccg cagcagaccg cgcgcaactc
     2281 cgcctgagcc gcatccggcc ggggttctag gcgagcgcgc gcgctatcgc cgccacgccc
     2341 tcttcgagat ccggcagcgc gcgcgtgtac gcgaaacgca cgtgctcgcg cgggcggttg
     2401 gatccgaagt cgatccccgg cgtgatcgcc accccggccc gctcgagcag gtcgagcgcc
     2461 aggtcccagc tgtcgccgaa gcgctcgcac ccggcgtaga tgtagaacgc cccctcgggc
     2521 acgagcggaa tccggaaacc caggcgccgc agcgcaggca ccatgaaatc gcggcgccgc
     2581 tcgaactcgg cgcggcgctc ctcgagcacg gccagcgtgt cgggccggaa cgcggcgagc
     2641 gccgcgtgct gcgagggcgc cggcgggcag atcgcgacgt tctgcgagag cttctcgacc
     2701 tcccgcgcgt agcgctcggg aacgaccatc catcccaggc gccagccggt catgttgaag
     2761 tacttcgaga agctcgccac gacgaagaca tcgtccgcga tcgacagcgc ggtcgaaggc
     2821 cgccgcccgt agaccagccc ctggtagatc tcgtcgacga cgaccgttcc gccccggcgg
     2881 cgggtctcgt cggcgatctc gcgcagcgcg tcgggctcga tcagcgtgcc ggtcgggtta
     2941 gacggcgacg cgagcatcac gcagcgagtc gcgccggtcc aggccgagcg cacgtcggcg
     3001 gcggtgagct ggtacgactg gcgctcgtcg accgccaccg tcctcggcac cgcgtcgaat
     3061 aggcgcacga aatggcggtt gcaggggtag cagggatcgg gcatcagcgc ctcgtcgccc
     3121 gggttgagca gcacgccgag cccgagcagc agcgcgcccg acgcgcccgt cgtgaccacg
     3181 atacgctcgg ccgggacgtc ggcgccgagg cgctcgcggt agtggcgcgc gatcgcctcg
     3241 cgcagctcgg gcagcccggc cgacgcggtg tagcccaggc gccgcgtgcg gatcgccgcg
     3301 acggccgcct cggcgacctg cgggggagcg ccgaagtccg gctgcccgat ctccatgtgc
     3361 acgatgcggc gcccttgcgc ctcgagcgcg tgggcgcgcg cctgcacgtc catcacgtgg
     3421 aacggctcga tcgaggccat ccgcgccgcg agtcgcggct cgtgttcgcg cttcatcgtg
     3481 tctccgacgg cgtccatgat accgcggccg ccgcgccgcg gtccgcggcg aatcggctat
     3541 gattccgctc ccgatcggag cagaaaccgc catggacgac tcggtgcgca agagcgcgct
     3601 cgactaccac cgcctgccga ggcccggcaa gatctcgatc gtccccacga agggcctcgt
     3661 gaaccagcgc gacctcgcgc tcgcctacac gccgggcgtc gcggccgcgt gcgacgagat
     3721 cgtgcgcgac ccggctcagg cgcacgcgct gacggcgcgc ggaaacctga tcggcgtggt
     3781 cacgaacggg actgcggtgc tcgggctggg cgccatcggg ccgctcgcct cgaagccggt
     3841 catggaaggc aagagcgtgc tgttcaagaa gttcgccggc atcgactgct tcgatatcga
     3901 gctgaacgag cgcgacccgg acaagctggt cgacatcatc gccgcgctcg agcccacctt
     3961 cggcggcatc aacctcgagg acatcaaggc gccggagtgc ttcctgatcg agaagaagct
     4021 gcgcgagcgc atgaagatcc cggtgttcca cgacgaccag catggcacgg cgatcatcgt
     4081 gggcgccgca gtcctcaacg gcctgcgcgt cgtcggcaag gacatcgcgc gcgtgaagct
     4141 cgtgtgctcg ggcgcgggcg cggccgcgct cgcctgcctc gacctgctcg tcgggctcgg
     4201 cctgtcgatg gagaacgtct gggtctccga catcaagggc gtggtctacg aaggccgcaa
     4261 ggaggagatg gacccgaaca aggcacgcta cgcgcgtaag acgtccgccc gcgcgctcgc
     4321 cgacatcatc tccgacgcgg acgtgttcct cggtctttcg gcgggcggcg tgctccagcc
     4381 cgagatggtg aagcgcatgg ccgaccggcc gctgattctc gccctcgcca atcccgagcc
     4441 cgagatccag cccgacatcg cgcgctcggc gcggcccgac gcgctgatcg cgaccggccg
     4501 ctcggactac ccgaaccagg tgaacaatgt cctgtgcttc cccttcatct tccgcggcgc
     4561 gctcgacgtc ggcgcgacgg cgatcaacga ggcgatgaag ctcgcggcag tgcgcgcgat
     4621 cgccgacctg gcgatggccg agcagtccga ggtcgtgacc gcggcctacg gcgagcagaa
     4681 cctgaagttc ggccccgact atctcatccc caagcccttc gacccgcggc tgatcgccaa
     4741 gatcgccccc gcggtggcga aagccgcgat ggactcgggc gtcgccacgc gcccgatcga
     4801 ggacctcgag gcgtacaagg accagctgca gcagttcgtc tacacctccg gcctgatcat
     4861 gaagccgatc ttcgccgccg ccaaggaagg cccgaagcgg cgcatcatct tcgccgaggg
     4921 agaggacgag cgcatgctgc gcgcggcgca ggtggtgatc gacgagggcc tcgcggcgcc
     4981 ggtgctgatc ggacggcccg ccgtggtcga gcgccggatc gagcgttacg ggctgcgaat
     5041 cgcgcccggc aaggacttcg agctcgtgaa ccccgagtac gatccgcgct tccgcgaata
     5101 ctgggaagag tacttcaagc tggtcgagcg gcgcggcgtc tcgccgcagt acgcgcagat
     5161 cgagatgcgc cgccatcaca ccctcatcgg cgccatggcg atccaccgcg gcgaggccga
     5221 cggcatgatc tgcggcgcct tcggcacgca cgagctgcac ctgcgctaca tcgagcaggt
     5281 catcggccgg cgcgccggcg tcaaccacta ttacgcgatg aacgtggtga tgctgccgaa
     5341 gcgcacggtg ttcatctgcg acacttatgt gaacctggac ccgaccgccg agcagatcgc
     5401 cgagatgacg gtgctcgccg cggaggaggt gcgccgcttc ggcgtcgcgc ccaagatcgc
     5461 gctgctgtct cattcgagct tcggcacgag cgacgcgccg agcgcgcgca agatgcgcga
     5521 ggcgctcgca ctgctcgagc ggaacgcgcc cgagctcgac atcgagggcg agatgcacgg
     5581 cgacgccgcg ctcaacgaga gcctgcggct ggcgagcttt ccacgctcgc gcctgaaagg
     5641 cgaggccaat gtgctgatca tgccgacgct ggacgccgcg aacatcgcct tcaacctgct
     5701 gaagacggcg gcgggcgacg gcatcacgat cggccccatc ctgctgggcg ccgcgcgtcc
     5761 ggtgcacatc ctcacgcctt cggcgaccgt gcggcggatc gtcaacgtca ccgcgctgac
     5821 ggtcgtcgac gtgaacgcgg cgagggagca gatgcggctg ttcgcaaggc cgggctagag
     5881 cggctaaact ccgcgcttcg cccacggatc tcagcgacaa gaacagcgcg gcgatcacca
     5941 tggaaccgac caagccgaaa gccgggctcg tcctcacggg cggcggcgcc cgcgcggcct
     6001 accaggtcgg cgtgctgaag gcggtgagcg agctgatggc gcggcccgag cgcaacccct
     6061 tccccatcct gtgcgggacc tcgtccggtg cgatcaacgc cgcgacgctc gccgtgttcg
     6121 cgaacaactt cggcgagggc gtcgagaagc tgctcgacgt atgggccgat tgccgcgtgc
     6181 accacgtcta ccgctcggac ccgctcgccg tggccaggtc cggggcgcgc tggctggcgg
     6241 cgatgatgct gatcgcgcgc tcgagcccgg tatcgctgct cgacaacacg ccgctcgccg
     6301 agctgctcgc gcgcagcctc gacttcggcc gcatccagca gaacatcgac gacggccacc
     6361 tctacgccgt gtcgatcacc tgctcgggct actcgtccgg gcagagcgtg agcttctacc
     6421 agggcggtcc gggcaccgag gcgtgggagc gcacgcagcg cgtcggcgcc gccatgccga
     6481 tcggggtcga gcacctcatg gcttcctcgg cgctgccgtt catctttccg gcggtgaaga
     6541 tcaaccgcga gtacttcggc gacggctcga tgcgccagat ggcccccgtg agcccggcgc
     6601 tgcacctcgg atcggaccgc gtgctcgtga tcggcacggg gcggcacgtc aacgtcgagc
     6661 cgccgcgggt gcgctcgagc ctctacccgt cgcttgcgca ggtcgcgggc catgcgctga
     6721 acagcatctt cctcgacagc ctctacgtgg acatcgagcg gctgcaccgc atcaaccgca
     6781 cgatctcgct gatcccggcc gagcgcctgc gcgaggcggg cctgccgctg cggaagatca
     6841 aggtgctggt catccagccg agccagccgc tcgagcgcat cgcggtgcgc ttcctgcccg
     6901 atctgccgtg gacgatacgc ttcctgctgc gcggcatcgg cgcgatgaac cgcaacggct
     6961 cgacgctggc gagctacctt ctgttcgaga agggcttctg ccaggcgttg atcgacctgg
     7021 ggtacgagga caccatggcg cggcgtcccg aggtactgga atttttggag gccgcgcact
     7081 agactggaag cgccccgcag cacgccccgc ccgccaaccc catgagccag tctccggacc
     7141 cgttcgaatt catgaagagc ctctgggcgc ccatgggatt gcccggggtg atggcgccga
     7201 cgctcgatgt cggcgaggtc gaccggcgca tcgccgagct gaagtcggtc gagaactggc
     7261 tgaacatgaa cctcgccgcg ctgcggatga cgatccaggg gctcgagatg cagcgtgcga
     7321 cgctcgccgc gatgcagggc ggcgcgcagg ccaagggctc tcccggcggc ggagatgcgg
     7381 gcgaggccgg ccgccggttc gccgaggcgt ggctgaagat gctgcagcag gcgccttccg
     7441 ccggctcggg atcgacgagc gccggggctt cctcttcggc cagccccggg aaggacaagg
     7501 agcccgggaa gaaatgagcc tcacgttccg gcacgcgcat gcgtcggcgc gtcgttggca
     7561 ggacgccgcc cgcggctgcg ccgagacgct cggctcgggg ccgcgcggag ccaacctcgg
     7621 cttcctctat ttcagcgacc actacgctgc gcacgccgcc gagctcctcg attacctgaa
     7681 ggaggagacc ggcgtcgacg agtgggtcgg cacggtcggg atcggcgtga tcgccaccgg
     7741 caccgaatac ctcgacgagc cggcgatgtc ggtgatgctg ggcgcgtttc cgcccgattc
     7801 ttaccgcgtc ttctcggggc gccagcgtcc gccgcgtgcc gggacgcgca cgctcacggg
     7861 ctcgctcgcg ggcaacttcg cggtcgtgca cggcgacccg caaacgcccg acattcccga
     7921 gctcatcgac gacatgtcgc gcaaggtgga cagcggcttc ctcgtcggcg ggctgtcgag
     7981 ctcgcgcacg cgcacggtgc agatcgcgaa cgaggtgctg cagggcgggc tctcgggcgt
     8041 gatcttctcg tcggacgtcg cgatcgcgac ccggctcacg cagggctgcg cgccgctcac
     8101 gcgctcggat acgcacgcgc cggttcgcca ccgcgtgacg tcgtgcgagc gcaacgtcat
     8161 cgccgccctc gacgaccggc cggcgctcga cgtgttccgc gaggagatcg gcgacgatct
     8221 cgcccgcgac ctgggccggg cggcgcgcca cgtgctcgtg ggcctttccg tcccgggatc
     8281 ggacaccggc gactacctcg cgcgcaacgt cgtcggcatc gatccgcgga acaagctgtt
     8341 cgcgatcgga gcgccggtcg agcagggcat gagcatcatg ttctgccgcc gcgacggcga
     8401 ggcggcgcgc gaggacctgg tgcgcatgct caaaggactg cgcgcggagc tgcgcggcgt
     8461 tccgcgcggc ggcctgtact actcgtgcat cgggcgcggc gcgcagatgt tcggccacaa
     8521 gtccgcggag ctcgagatca tccgccagga gctcggcgat ttccccatgg tcgggttctt
     8581 ctgcaacggc gagatctcgc acgaccggct ctacggctac accggcgtgc tcactttgtt
     8641 cgtttgaacg ctgcttttca accaggagga gtgatgccga aggcgattcg aatccagaag
     8701 cagggcgggc ccgaagtcat gcagtgggag gacgtgcagg tcggcgatcc cggcccgggc
     8761 gaggcgcgcg tgcgccacaa ggccgtcggc ctgaactacc tcgacaccta tcagcgcggg
     8821 ggcctgtaca agatcgcg
//
LOCUS       DATDMW010000282         6852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_956770, whole genome shotgun sequence.
ACCESSION   DATDMW010000282 DATDMW010000000
VERSION     DATDMW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956770"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VI319_12100"
     CDS             complement(<1..482)
                     /locus_tag="VI319_12100"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6864630.1"
                     /translation="MKNALAFATAVTAVVAAAGNAAAQTYPSRPVTLIVGFAPGGGTD
                     TVARVMQKKVGEYLGQTVVVENRAGAGGTIAAAVVAKAEPDGYTILLATIAALAVAPH
                     LNSKLPYNPLKDFAPISMATESGNVLVVHPSVPAKTLAEFVKLANSKPGGIAYGTSGV
                     G"
     gene            complement(496..1338)
                     /locus_tag="VI319_12105"
     CDS             complement(496..1338)
                     /locus_tag="VI319_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY6864631.1"
                     /translation="MTPQEIQALPRQSVFAPGALTERLVVEKQGPVGWLTFNNPAKRN
                     AVSIDMWEGIPRALERFEQDPAIRVIVLSGAGDKAFVSGADISQYEKDRSTAEGIQRY
                     NRISSAACERLEACDKVTLAMIRGYCLGGGVNVALCCDLRLAADDARFGVPAAKLGLG
                     YGAGSLKNLVDTIGAAYAREVLITGRQFGADEAKAMGFVHRVAAAAALEALALEYCAT
                     VSENAPLTIRAAKRIVRELLQPNDAFDADACAAWVRECFESQDYVEGRRAFMEKRKPA
                     FQGK"
     gene            complement(1400..2071)
                     /locus_tag="VI319_12110"
     CDS             complement(1400..2071)
                     /locus_tag="VI319_12110"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEY6864632.1"
                     /translation="MEMLALAALAFLATHGVASTPLRGALVRSLGERAYRGLYVVAAF
                     ATIGWMIWAFRRSPFVPLWDGGEFKAWAIALMPLVFILLVCGVTVRNPTAVGQEGALR
                     STDDPRGILRVTRHPVLWAIGLWALVHLAARGDAASAIFFGAFAVLALTGTMLIDARK
                     RRTLGVEWTRFADVTSNVPFAAIARGRNALRPAEIGWGRPLAGLALFAVVLLLHPWLF
                     GARPW"
     gene            complement(2117..4414)
                     /locus_tag="VI319_12115"
     CDS             complement(2117..4414)
                     /locus_tag="VI319_12115"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6864633.1"
                     /translation="MNAPITAISRREFLKASGALIVSASLPGFVAEALAQADAPAGAF
                     GATRPAVVPTELDSWIAVGADGRVTAFFGKMDMGQSLDIAIAQIVAEELDVNVEKVDV
                     LMGDTALTCNQGGASGSTGIFEGAKPLRKAAAEARRVLVGAASSKLGVPAESLRVDDG
                     VVAAVSDPSKKATYAELIGGKYFNHPVEWNKKIGNPMDIEVPAKVKSPSEYRVVGKSP
                     VRRDLVSKVYRTEPYVTDVSIPGMLHARVVRPERAGCAVGSVDEASIAAVKGARLVQE
                     KNFVAVVAEREWDAVKASRALKVNWTETHKDPFPPMADLHEHIRRAPVVHREEPVKKG
                     DVGSAFKRAARVVEAEYEWPFQSHASMGPGCAVADVRDDGVTVWSGTQKPHFTQEGVA
                     RIVGVAPEKVRVIWVRGPGSYGRNDAGDAALDAALLSKLTGKPVRVQGMRRDGIAWDP
                     KGPASVHRARAALDASGNVLGYEFTSKAFSRVNIATNEANPQDSLAGMEIGVAPKPGI
                     GYGNPSDSYGFENKQYAWEVIAPLLDRCSPLRTSHLRDPVGPDIHFAAEQFIDELAVA
                     AKMDPVEFRLKHVTAPRDAAVIKAAAEKAGWKPRPGPRARRSGDVMRGQGIAYATRGG
                     TIVAVVANIEVERKTGRIWARRVTVAHDCGLVINPTGLRYTIEGNVGHGLSRALFEEV
                     RFDRDKVTSVDWMSYPVLEIRDAPETVDVVVINHPEIAPTGAGEPTIRVMPAAVANAF
                     YDATGVRIRRAPLTAERVKAALAKA"
     gene            complement(4425..4889)
                     /locus_tag="VI319_12120"
     CDS             complement(4425..4889)
                     /locus_tag="VI319_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6864634.1"
                     /translation="MATFKFNVDGKPVSVDADPDMPLLYALRGDLGMTNPHFGCGLAQ
                     CGACTVHLNGEAIRSCVMPVANVQGKKVTTLKGLGTPEKPHPLQAAYVQEQVPQCGYC
                     INGWIMTAAAYLKKNPKASDAQIRDALAGLKCRCGTHVSILRAVKRAQKQMA"
     gene            4988..6532
                     /locus_tag="VI319_12125"
     CDS             4988..6532
                     /locus_tag="VI319_12125"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY6864635.1"
                     /translation="MKPEKPGPQERVDCERYRLRRFVEALQQSGDARLVPGPADLADV
                     APALEGSDSAVLFERVGPEGAQLVGNVMGSRARLARAFGVAPEGLLAEVLRRARLAPE
                     VIEVPREQAPVQEVVWTGADADLTKLPANLQHGMDGAPYLSASIDYVRDPASGWTNVG
                     VRRLMLRGRTEAGVDLNAPSDLRAIYEASANRGEPLPVAFVVGAHPVDYVAATLRLPV
                     DELGLVASLRAAPLPVVKCVTSGIRVPADAEWVLEGTLDARGHAEAEGPYGEFLGYYG
                     AVKQNPVFHLAAITRRRDALFQTATIGGRLLGRTDTAQLNGLRTEVMVWRALETAVRE
                     PLAVHATPSSGGMFNLRVALRSRVPGEARNAIAAAFGSLANVKHVFVVDPDIDVFSDA
                     QMDWALATRFQADRDLVVQAGFRALPLDPSLAGARTGAKAGFDCTLPFGQAQSIEHRI
                     PEPPRFDGKRFASVEAALADGPKRFEQLMAATGSRDGREIVLVLESLRSGGRLARDAE
                     GRYGLK"
     gene            6529..>6852
                     /locus_tag="VI319_12130"
     CDS             6529..>6852
                     /locus_tag="VI319_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6864636.1"
                     /translation="MTRYVGRSVPRVEDQRFLTGQGRYTDDLSLEGQLHGVFVRSPHA
                     HARIVRVDAGATPGVTVLTGADYAADGLEGIRHVPNPADALDVSKRAFSGRVVDLPHF
                     PLAAER"
BASE COUNT          924 a         2470 c         2444 g         1014 t
ORIGIN      
        1 ccgacgcccg aggttccgta ggcgatcccg cccggcttgc tgttcgcgag cttcacgaac
       61 tcggcgagcg tcttggcggg caccgacggg tgcaccacca gcacgttgcc cgactcggtc
      121 gccatcgaga tcggcgcgaa gtccttgagg gggttgtacg ggagcttgga gttcaggtgc
      181 ggcgcgaccg cgagcgcggc gatggtcgcg agcaggatcg tgtagccgtc gggctcggct
      241 ttggcgacga ccgcggcggc gatcgtccct cccgcgccgg cgcggttctc gacgaccacg
      301 gtctggccca ggtactcgcc caccttcttc tgcatgacgc gtgcgacggt gtcggtgccg
      361 ccgcccgggg cgaagcccac gatcagcgtg accggacggc tcgggtaggt ctgcgcggcg
      421 gcgttgccgg ccgcggcgac gacggccgtg acggccgttg cgaacgcgag tgcattcttc
      481 atctcgttct cctcttcatt tgccctggaa cgcgggcttg cgcttctcca tgaaggctct
      541 gcggccttcg acgtagtcct ggctctcgaa gcattcgcgc acccaggccg cgcacgcgtc
      601 ggcgtcgaag gcgtcgttcg gctggagcag ctcgcgcacg atgcgcttcg ctgcgcggat
      661 ggtgagcggc gcgttctccg agaccgtcgc gcagtactcg agcgcgagcg cctcgagcgc
      721 ggcggccgcc gcgacccggt gcacgaatcc catggccttc gcctcgtcgg ccccgaactg
      781 ccgcccggtg atcagcacct cgcgcgcgta cgccgcgccg atcgtgtcca ccaggttctt
      841 gagcgagccc gcgccgtagc cgaggccgag cttcgccgcc ggaacgccga agcgcgcgtc
      901 gtccgcggcg agccgcagat cgcagcacag cgcgacgttc acgccgccgc cgaggcagta
      961 gccccggatc atggcgagcg tcaccttgtc gcaggcctcg agccgctcgc aggccgcgct
     1021 cgagatgcgg ttgtagcgct ggatgccctc agcggtcgag cggtccttct cgtactgcga
     1081 gatgtcggcg cccgagacga acgccttgtc gcccgcgccc gagaggacga tcacgcggat
     1141 cgccgggtcc tgctcgaagc gctcgagcgc ccgcgggatg ccctcccaca tgtcgatcga
     1201 gaccgcattg cgcttcgccg ggttgttgaa cgtcagccag ccgaccggtc cctgcttctc
     1261 gacgacgagg cgctcggtga gggcgcccgg agcgaacacg ctctggcgcg ggagggcttg
     1321 gatttcctgg ggagtcatcg ggggttcggc cgggcaggcc cattgtaagg ggccttccgg
     1381 gatgcgtcac tcccgcgcat caccaggggc gcgcgccgaa cagccacggg tggaggagca
     1441 gcaccacggc gaagagcgcg agccccgcga gcggccggcc ccagccgatc tccgcgggcc
     1501 gcagcgcgtt gcgcccgcgc gcgatcgcgg cgaacgggac gttcgaggtc acgtcggcga
     1561 agcgcgtcca ctcgaccccg agcgtgcggc gcttgcgcgc gtcgatcagc atcgtccccg
     1621 tgagcgcgag aacggcgaag gcgccgaaga agatcgccga cgccgcgtcg ccgcgcgcgg
     1681 cgaggtgcac cagcgcccac aggccgatcg cccacaggac gggatggcgc gtgacccgca
     1741 ggatgccgcg cggatcgtcc gtcgagcgca gcgcgccttc ctggccgacc gcggtcggat
     1801 tgcgcacggt gacgccgcac accagcagga tgaagacgag cggcatgagc gcgatcgccc
     1861 acgccttgaa ctcgccgccg tcccagagcg ggacgaaggg cgagcgccgg aacgcccaga
     1921 tcatccagcc gatcgtggcg aacgcggcga cgacgtagag gccgcggtac gcgcgctcgc
     1981 cgagcgagcg caccagcgcg ccgcgaagcg gcgtgctggc gacgccatgc gtcgcgagga
     2041 acgccagcgc ggcgagcgcg agcatctcca tggagcctcc ctccccgcaa aacccgcctc
     2101 gcggcgggtc tgttcttcag gccttcgcca gcgccgcctt cacccgctcg gccgtgagcg
     2161 gcgcgcgccg gatgcgcacg ccggtcgcgt cgtagaacgc gttcgcgacc gccgccggca
     2221 tcacgcggat ggtcggctcg cccgccccgg tcggcgcgat ctccgggtga ttgatgacga
     2281 cgacgtcgac cgtctcggga gcgtcgcgga tctcgagcac cgggtagctc atccagtcga
     2341 cgctggtgac cttgtcgcgg tcgaaccgga cctcctcgaa cagcgcgcgc gacagtccgt
     2401 gcccgacgtt gccctcgatg gtgtagcgca ggccggtcgg attgatcacc aggccgcagt
     2461 cgtgcgccac ggtgacgcgc ctcgcccaga tgcggccggt cttgcgctcg acctcgatgt
     2521 tcgcgacgac cgcgacgatg gtgccgccgc gcgtcgcgta cgcgatgccc tggccgcgca
     2581 tcacgtcgcc cgagcggcgc gcgcgaggcc cgggacgcgg cttccatccc gccttctcgg
     2641 ccgcggcctt gatgacggcg gcgtcgcgcg gcgcggtgac gtgcttcagc cggaactcca
     2701 cgggatccat cttcgccgcg acggcgagct cgtcgatgaa ctgctcggcc gcgaaatgga
     2761 tatcgggccc gacggggtcg cgcaggtggg acgtccgcaa cggcgagcag cgatcgagca
     2821 gcggcgcgat gacctcccag gcgtactgct tgttctcgaa gccgtacgaa tccgaggggt
     2881 tgccgtagcc gatcccgggc ttcggcgcca cgccgatctc catgccggcg aggctgtcct
     2941 gcgggttcgc ctcgttggtc gcgatgttga cgcgcgagaa cgccttgctc gtgaattcgt
     3001 agccgagcac gttgcccgac gcgtcgagcg cggcgcgcgc gcgatgcacc gacgccggcc
     3061 ccttcgggtc ccaggcgatg ccgtcgcggc gcatgccctg cacgcgcacc ggcttgcccg
     3121 tgagcttcga cagcaaggcc gcgtcgagcg ccgcgtcgcc cgcgtcgttg cgcccgtacg
     3181 agccggggcc ccgcacccag atgacgcgca ccttctccgg ggccacgccg acgatcctgg
     3241 cgacgccctc ctgcgtgaag tgcggcttct gcgtgcccga ccagacggtg acgccgtcgt
     3301 cgcgcacgtc ggccacggcg cagccgggac ccatgctcgc gtgcgactgg aacggccact
     3361 cgtattcggc ctcgaccacg cgcgcggcgc gcttgaacgc cgagccgacg tcgcctttct
     3421 tcaccggctc ctcgcgatgc acgacgggcg cgcgccggat gtgctcgtgc aggtccgcca
     3481 tcggcgggaa cggatccttg tgcgtctcgg tccagttcac cttgagcgcg cgcgaggcct
     3541 tcaccgcgtc ccactcgcgc tcggcgacca cggcgacgaa gttcttctcc tggaccaggc
     3601 gcgcgccctt cacggccgcg atcgacgctt cgtcgaccga cccgaccgcg cacccggcgc
     3661 gctcgggacg cacgacgcgc gcatgcagca ttcccgggat cgagacgtcg gtcacgtagg
     3721 gctcggtgcg gtacaccttc gatacgaggt cgcggcgcac gggcgacttg ccgacgacgc
     3781 ggtactcgga gggcgacttg accttcgccg gcacctcgat gtccatcggg ttgccgatct
     3841 tcttgttcca ctcgacgggg tgattgaagt acttgccgcc gatcagctcg gcgtaggtcg
     3901 cctttttcga cgggtccgag accgcggcga cgacgccgtc gtcgacgcgc aaggactccg
     3961 ccggcacgcc gagcttcgac gacgccgcgc cgaccagcac gcggcgcgct tcggccgccg
     4021 ccttgcgcag gggcttcgcg ccttcgaaga tgccggtcga gcccgaggcg ccgccctggt
     4081 tgcaggtgag cgcggtgtcg cccatgagga cgtcgacctt ctcgacgttc acgtcgagct
     4141 cctcggccac gatctgcgcg atcgcgatgt cgagcgactg gcccatgtcc atcttgccga
     4201 agaaggcggt gacgcggccg tcggcgccga ccgcgatcca ggagtcgagc tcggtcggga
     4261 ccaccgcggg cctggtcgcg ccgaaggcgc cggcgggcgc gtcggcctgc gccagcgcct
     4321 cggcgacgaa gccggggagc gacgcgctca cgatcagcgc gcccgaggcc ttgaggaatt
     4381 cgcggcggga aatcgcagtg atgggtgcgt tcatcgtcgt ctcctcaggc catctgcttc
     4441 tgcgcgcgct tcacggcgcg caggatcgac acgtgcgtgc cgcagcggca cttcaggccc
     4501 gcgagcgcgt cgcggatctg ggcgtcgctc gccttggggt tcttcttgag gtaggcggcg
     4561 gcggtcatga tccagccgtt gatgcagtag ccgcactgcg gcacctgttc ttggacgtac
     4621 gcggcctgga gcggatgggg cttctccggc gtgccgagcc ctttcagcgt ggtgaccttc
     4681 ttcccctgca cgttcgcgac cggcatcacg caggatcgga tcgcctcgcc gttcaggtgc
     4741 acggtgcacg cgccgcactg cgcgaggccg cagccgaagt gcgggttggt catgccgagg
     4801 tcgccgcgca gcgcgtacag cagcggcatg tcgggatcgg cgtcgacgga caccggcttg
     4861 ccgtcgacgt tgaacttgaa agtcgccatg gggttcctcc ttggagtcgg ggtcagttta
     4921 ttccccgccg cgcgggagtt gcaatcgcgg cccggtgata cgataggcgc cgtcgcgaag
     4981 gaggagcatg aagccggaga agcccggtcc gcaggagcgc gtcgattgcg agcgctaccg
     5041 cctgcggcgg ttcgtggaag cgctgcagca atcgggcgac gcgcgcctcg tgcccgggcc
     5101 ggccgacctg gccgacgtcg cgccggcgct ggaaggcagc gatagcgcgg tcctgttcga
     5161 gcgcgtcggt ccggaagggg cgcagctcgt cggcaacgtc atgggaagcc gcgcgcggct
     5221 cgcgcgcgcc ttcggcgtcg cgcccgaggg cctgctcgcc gaggtgctgc gccgcgcgag
     5281 gctcgccccg gaggtgatcg aggtgccgcg cgagcaggcg ccggtccagg aggtcgtctg
     5341 gacgggcgcc gacgccgacc tgacgaagct gccggcgaac ctgcagcacg ggatggacgg
     5401 cgccccgtac ctctcggcct cgatcgatta cgtccgcgat ccggcgagcg gctggaccaa
     5461 cgtcggggtc cgccgcctga tgctgcgcgg ccgcaccgag gccggcgtcg acctgaacgc
     5521 gccgtcggac ctgcgcgcga tctacgaggc gagcgcgaac cggggcgagc cgctgcccgt
     5581 cgccttcgtc gtcggcgcgc acccggtcga ctacgtcgcg gcgacgctgc ggctgccggt
     5641 cgacgagctg ggcctggtcg cgagcctgcg cgcggcgccg ctgcccgtcg tgaagtgcgt
     5701 gaccagcggc atccgcgtgc cggcggacgc cgagtgggtg ctcgagggaa cgctcgacgc
     5761 ccgcggccac gccgaggccg aaggcccgta cggcgagttc ctcggctact acggcgcggt
     5821 gaaacagaac ccggtcttcc atctcgccgc gatcacgcgc cggcgcgacg cgctgttcca
     5881 gaccgcgacc atcggcgggc ggctgctcgg ccgcaccgac accgcgcagc tgaacggcct
     5941 gcgcaccgag gtgatggtgt ggcgcgcgct cgagaccgcg gtgcgcgagc cgctcgcggt
     6001 gcacgcgacg ccgtcctcgg gcggcatgtt caacctgcgc gtggcgctgc gcagccgcgt
     6061 cccgggcgag gcgaggaacg cgatcgccgc cgcgttcggc tcgctcgcga acgtcaagca
     6121 cgtgttcgtc gtcgaccccg acatcgacgt cttctccgac gcgcagatgg actgggcgct
     6181 cgcgacgcgc ttccaggccg atcgcgatct cgtcgtgcag gccggcttcc gcgcgctgcc
     6241 gctcgatcct tcgcttgcgg gcgcgcgcac cggcgccaag gcggggttcg actgcacgct
     6301 gccgttcggc caggcgcagt cgatcgagca ccggatcccc gagccgccgc gtttcgacgg
     6361 caaaaggttc gcctccgtcg aggccgcgct cgccgacggc ccgaagcgct tcgagcagct
     6421 gatggcggcg accggcagcc gcgacgggcg cgagatcgtg ctcgtcctgg aatcgctgcg
     6481 ctcgggcggc cggctcgccc gcgacgccga agggcggtac gggctcaagt gacgcgctac
     6541 gtcggccgct ccgtcccgcg cgtcgaggat cagcgctttc tcaccgggca aggccgctac
     6601 accgacgatc tctcgctcga aggccagctg catggcgtgt tcgtgcgctc gccgcacgcg
     6661 cacgcgcgca tcgtccgcgt cgacgccggc gcgacgccgg gcgtgacggt gctcaccggc
     6721 gccgactacg ccgccgacgg cctggaaggc atccgccacg tcccgaaccc cgccgacgcg
     6781 ctcgacgtgt cgaagcgcgc gttctccggc cgcgtggtcg acctgccgca ttttccgctc
     6841 gcggcggagc gc
//
LOCUS       DATDMW010000283         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_460561, whole genome shotgun sequence.
ACCESSION   DATDMW010000283 DATDMW010000000
VERSION     DATDMW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460561"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VI319_12135"
     CDS             complement(<1..1222)
                     /locus_tag="VI319_12135"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY6864637.1"
                     /translation="MIRVSLSPLSNAALLLALFAALEIGGLGLLFPLEGRFLDALVRT
                     HASGLEPDPDIVIVDIDEASLAKMSEVAGKFPWPRSVYADLVLGIEGQRPRAIVFDLF
                     FSEEDFYNREGDRAFNAALKGLSNVYFPIQRLDESTDANGVPIARVAKVLALGRTDRA
                     QPNARIQLLPPQALDEVHWRRSGIVNFVEDTDGVGRRYWLDMPAYGWRLPSLPARVAR
                     DLGFPVPPGEDIVLNWRGRARAFQHISYADLYEDLLRKNPKRPLDELRNKIVIVGVTA
                     TALGDRRVTSLSNVYPGVEILATALDNLKNRRWMRPTPGYLEWGLTLLLLAFVWEAFR
                     LRRYPLEIGVALGVISGVLVAGSYIALGAERLVPVFTPLVFAWSLYLYGAVLAYISER
                     RHRQHAVRMFSRFLN"
     gene            complement(1238..1801)
                     /locus_tag="VI319_12140"
     CDS             complement(1238..1801)
                     /locus_tag="VI319_12140"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEY6864638.1"
                     /translation="MSGHGHGHVDPSNKKVALLISVLAVVLAFAETLGKGAQTNGLAA
                     NIEASNLWAFYQAKTIRMTTLRTAAESAEVDLPASGTKKDALAKKIADWKSTAARYDS
                     EPETGEGRKELSARAKVAESKRDRAMAAYHQYEMASALVQIAIVLASAHIITGVGALV
                     WISGLLGLGGVAFCLIGFLAPMSVHLF"
     gene            1896..2426
                     /locus_tag="VI319_12145"
     CDS             1896..2426
                     /locus_tag="VI319_12145"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY6864639.1"
                     /translation="MNDAVSPGAGAGPLRLILSRRPGNAIGFLVCAGLLAFAYYLQFA
                     RGLEPCPLCILQRVVFAAMAIFFLVAAIHRPRRVGAWIYGVLLFALAVTGTAIAGRHV
                     WLQHTPEAERPSCGPGLDYLLSRFGPLGGLQRILRGSGECGAVDWTFLGLSIPEWSLA
                     CFIALGIYALVLAARD"
BASE COUNT          381 a          860 c          879 g          408 t
ORIGIN      
        1 ggttcagaaa gcgcgagaac atgcgcaccg cgtgctggcg atgccggcgc tcgctgatgt
       61 atgcgagcac cgcgccatag agataaagcg accatgcgaa gacgagcggc gtgaacaccg
      121 gcaccaggcg ctcggcgccg agcgcgatgt aggaccccgc cacgaggact cccgagatga
      181 cgccgagcgc aaccccgatc tcgaggggat agcgacgcag gcggaaggct tcccagacga
      241 aggcgaggag cagcagcgtg aggccccact cgaggtagcc cggcgtcggg cgcatccagc
      301 ggcggttctt gaggttgtcg agcgccgtgg cgaggatctc cacccccggg tacacgttgg
      361 agagcgacgt cacgcgccgg tcgccgagtg ccgtcgcggt cacgccgaca atcacgatct
      421 tgttcctcag ctcgtccagc ggacgcttcg gattcttgcg caacagatcc tcgtacaggt
      481 cggcatacga aatgtgctgg aacgcgcgcg cccgtccgcg ccagttcagc acgatgtcct
      541 cgcccggcgg gacgggaaag cccagatcgc gggcgacgcg cgccggcagc gacggcaggc
      601 gccatccgta cgcgggcatg tcgagccagt agcgacggcc gacgccgtcc gtgtcctcca
      661 cgaaattgac gatgccgctg cggcgccagt gaacctcgtc gagcgcctgc ggtgggagca
      721 gctggatgcg cgcgttcggc tgcgcgcggt cggtccggcc caaggcgagc accttggcta
      781 cccgggcgat cggcacgccg ttggcgtcgg tgctctcgtc gagtcgctgg atcgggaagt
      841 acacgttgct gagacccttg agcgcggcgt tgaacgcgcg gtcgccttcg cggttgtaga
      901 aatcctcttc gctgaagaac aggtcgaaga cgatggcgcg cggacgctgc ccctcgatgc
      961 caagtaccag gtcggcatac acggagcgcg gccaggggaa ctttccggcg acctcggaca
     1021 ttttcgcgag gcttgcctcg tcgatatcga cgatgacgat atccggatcg ggctcgagcc
     1081 ccgacgcgtg cgttctgacc agcgcgtcga gaaagcgtcc ctcgagcgga aacagcagcc
     1141 ccaggccgcc gatctcgagc gccgcgaaga gcgcgagcag gagcgccgcg ttcgacagcg
     1201 gcgagagcga gacgcggatc accgcgcgcc cggcgcgcta gaacaggtgg accgacatcg
     1261 gcgcgaggaa gccgatgagg cagaacgcga cgccgcccag cccgagcagg cccgagatcc
     1321 acacgagcgc gccgacgccc gtgatgatgt gcgccgaggc gagcacgatc gcgatctgca
     1381 ccagcgccga cgccatctcg tactggtggt acgcggccat tgcgcggtcg cgcttgctct
     1441 cggcgacctt ggcgcgcgcc gagagctcct tgcggccctc gccggtctcg ggctcggagt
     1501 cgtagcgcgc ggcggtgctc ttccagtctg cgatcttctt cgcgagcgcg tccttcttcg
     1561 tgccgctcgc cgggaggtcg acctcggcgc tctcggcggc ggtgcgcagc gtcgtcatgc
     1621 ggatggtctt ggcctggtag aacgcccaga gattggacgc ctcgatgttg gccgccaggc
     1681 cgttcgtctg cgcgcccttg ccgagcgtct cggcgaaggc gaggacgacg gcgagcaccg
     1741 agatgagcag cgcgactttc ttgttcgacg ggtcgacgtg cccgtgaccg tggcccgaca
     1801 tggcggtctc cctgaattgt tgtcagccgg aattctgacg gctttccggt ccgcgcaaaa
     1861 gaggaagtgt aaactcccgg tatggaggct ggttcatgaa cgacgccgta tcccccggcg
     1921 cgggcgccgg gcccctgcgc ctcattctct cgcgccgtcc cggcaacgcg atcgggttcc
     1981 tggtgtgcgc ggggctgctc gcgttcgcgt actacctgca gttcgccagg ggcctcgagc
     2041 cctgcccgct gtgcatcctg cagcgcgtgg tgttcgctgc gatggcgatt ttcttcctgg
     2101 tcgccgcgat ccaccgccct cgccgggtcg gcgcgtggat ctacggcgtg ctgctcttcg
     2161 cgctcgcggt gacgggcacg gccatcgccg gccggcacgt ctggctgcag cacacgcccg
     2221 aggccgagcg cccctcgtgc ggtccgggac tggactacct cctctcgcgc ttcgggccgc
     2281 tcggcggcct gcagcgcatc ctgcgcggct cgggcgaatg cggggcggtc gactggacct
     2341 tcctcggact gagcatcccc gagtggtcgc tcgcatgctt catcgcgctc ggcatctacg
     2401 cgctcgtgct cgcggcgcgc gattgagccg gcggcgctaa ggccggcgct tttccagcgt
     2461 cggcgtccag ctcacgccgt ccggagaccg gtcttccagc gtcggggtcc agcgcacgcc
     2521 cgcgtccg
//
LOCUS       DATDMW010000284         3495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1706110, whole genome shotgun sequence.
ACCESSION   DATDMW010000284 DATDMW010000000
VERSION     DATDMW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706110"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..999
                     /locus_tag="VI319_12150"
     CDS             16..999
                     /locus_tag="VI319_12150"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864640.1"
                     /translation="MRSVAVLVLALAFLAHFLPEAAHAQDPAASYPSKPIRIFGQGTG
                     STADYLSRYLAQKLQERWGQPVIVDSRAGAGGTIPTDVVAKSAPDGYNMVMGHAGPFV
                     SAVTLYEKTLPYDPVKDFEPVTMVATGVTVLVVNPAVPVKTAREFVEYAKRKGDLSYA
                     SAGNGSISHLTGELLKSVTGIPLQHVPYRSAGNALTAVLSGEVPVSFLSPLTAHAQLA
                     AGKLRPLAVSSAERFPGAPEVPSATEAGIPGMVAKLWFGLFAPAKTPRPIVLKLNREI
                     GDILRAKETQEALLKQGVQAAPSTPEELAEWVRTEVARWTPLIRAAGIKAD"
     gene            1014..1994
                     /locus_tag="VI319_12155"
     CDS             1014..1994
                     /locus_tag="VI319_12155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6864641.1"
                     /translation="MKYRKLGKNGPTVSAIGLGAGSATTNFGERDDQVQIETMQRAVD
                     LGVTFFDTADRYMKGRHERLLGQALKGRRNQVILATKFGNFDLPDGKKGYNGRPAYVR
                     EACDASLKNLGIDHIDLYYLHRVDPEVPIEETVGAMSRLVEAGKVRYLGLSEVGKSLE
                     RACKVHPIAALQTEYSLWARDVEAEILPECRRLGIGFVPYSPLGRGLLTGNVRSLEDL
                     APHDLRRKQPRFQPGNLEKNLKLLEPIERIAADHDATQAQVALAWLLAQGDDIVPIPG
                     TKQQKWLEQNVAAVDLALSKPELALLAETFKPGARAGERYAPGYFVTLGA"
     gene            2017..3117
                     /locus_tag="VI319_12160"
     CDS             2017..3117
                     /locus_tag="VI319_12160"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6864642.1"
                     /translation="MSAVPKPEKDPRVEAAISHWAPRFVANGVPLSDFEQVTAACERW
                     EDWCRLWSERAAIHEKLGDEAAAKGKKLSAGAHWTRAAVCYHFGKFLFVIDMEQLKRA
                     HAKAVECRNKALPFLDPPGERVAIPYEGKTLYGNLRKPAFSSRSVAKPPVVVMCMDLD
                     SAKEEMDDYENRFLARGLATLAFDGPGQGEAEYDFAICPEYEKPVKAVVDFVETRKDL
                     DANRIGVWGVSLGGYYAPRAAAFEKRFKACVALSGPYARSASFEGRPSINVETFRVRS
                     KSANLEEAGKVALRMTLKGAAKNITCPIYIVAGTKDRLTPHTHAEQLAAEVSGPCVLS
                     VIEGGNHVVNNLWYRYRDQTADWMADQLGAGR"
     gene            3117..>3495
                     /locus_tag="VI319_12165"
     CDS             3117..>3495
                     /locus_tag="VI319_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6864643.1"
                     /translation="MPCHPERAARRICFSLALALLSAFAHAQPFPSRPLHLIVPFPPG
                     GSTDILARALAQKMSEGLGQPVVIDNRGGAGGTIGAAEAAKAAPDGHTIMMGHLGTLA
                     VAPAIYRKLPYDPVRSFAPVALMA"
BASE COUNT          561 a         1242 c         1201 g          491 t
ORIGIN      
        1 tctgaggagg tccgaatgcg atccgtcgcc gtgctcgtcc tggctctcgc tttcctcgct
       61 cattttcttc ccgaagccgc gcacgcccag gatcccgccg cgagctaccc ctcgaagccg
      121 atccgcatct tcggccaggg cacgggctcg accgcggact atctctcgcg ctacctcgcg
      181 cagaagctgc aggagcgctg gggacagccg gtgatcgtcg actcgcgcgc cggcgcgggc
      241 ggcacgatcc ccaccgacgt cgtcgcgaag tcggcgcccg acggctacaa catggtgatg
      301 gggcacgcgg gcccgttcgt gtcggcggtg acgctctacg agaagacgct gccctacgac
      361 ccggtgaagg acttcgagcc ggtgaccatg gtcgccaccg gcgtgaccgt gctcgtcgtc
      421 aacccggcgg tcccggtgaa gaccgcgcgc gagttcgtcg agtacgcgaa gcgcaagggc
      481 gacctctcgt acgcctcggc ggggaacggc tcgatcagcc acctcacggg cgagctgctg
      541 aagagcgtga ccggcatccc gctccagcac gtgccctacc gcagcgcggg caacgcgctc
      601 accgcggtgc tctcgggcga ggtgccggtg tcgttcctgt cgccgctcac cgcgcacgcg
      661 cagctcgccg ccggcaagct gcgcccgctc gccgtgtcga gcgccgagcg cttccccggc
      721 gcgcccgagg tgccgagcgc gaccgaggcg ggcattcccg ggatggtggc gaagctctgg
      781 ttcggcctgt tcgcgccggc gaagacgccg cggccgatcg tgctcaagct gaaccgcgag
      841 atcggcgaca tcctgcgcgc gaaggagacg caggaggcgc tgctcaagca gggcgtgcag
      901 gccgcgccgt cgacgcccga ggagctcgcg gagtgggtgc gcaccgaggt cgcgcgctgg
      961 accccgctga ttcgcgccgc cggcatcaag gccgactagc cggtcatcct tttatgaaat
     1021 accgcaagct cggcaagaac ggccccacgg tctccgccat cggcctcggc gccggcagcg
     1081 cgaccacgaa cttcggcgag cgcgacgacc aggtgcagat cgagacgatg cagcgcgcgg
     1141 tcgacctcgg cgtcaccttc ttcgacaccg ccgaccgcta catgaaaggc cggcacgagc
     1201 ggctgctcgg ccaggcgctc aaaggccgca ggaaccaggt gatcctcgcg accaagttcg
     1261 gcaacttcga cctgcccgac gggaagaagg gctacaacgg caggcccgcg tacgtgcgcg
     1321 aggcgtgcga cgcgagcctg aagaacctcg ggatcgatca catcgatctc tactacctgc
     1381 accgcgtcga ccccgaggtg ccgatcgagg agacggtcgg cgcgatgagc cggctggtcg
     1441 aggcggggaa ggtgcggtac ctcggcctct ccgaggtcgg caagtcgctc gagcgcgcgt
     1501 gcaaggtgca cccgatcgcg gcgctgcaga ccgagtactc gctctgggcg cgcgacgtcg
     1561 aggccgagat cctgcccgag tgccgccggc tggggatcgg cttcgtgcct tactctccgc
     1621 tgggccgggg gctcctcacc gggaacgtgc gctcgctcga ggacctcgcg ccgcacgacc
     1681 tgcggcgcaa gcagccgcgc ttccagccgg gcaacctcga gaagaacctg aagctgctcg
     1741 agccgatcga gcggatcgcg gccgaccacg acgcgaccca ggcgcaggtc gcgctcgcct
     1801 ggctgctggc gcagggcgac gacatcgtcc cgatccccgg caccaagcag cagaagtggc
     1861 tcgagcagaa cgtcgccgcg gtcgatctcg cgctctcgaa gcccgagctc gccctcctcg
     1921 ccgagacctt caagcccggc gcgcgcgcgg gagagcgcta cgcgcccggc tatttcgtga
     1981 cgctcggtgc atagcttcgt agaggagaac cgaaccatgt ccgccgtccc caaacccgaa
     2041 aaagaccccc gcgtcgaggc cgcgatctcg cactgggccc cgcgcttcgt cgcgaacggc
     2101 gtgccgctct ccgacttcga gcaggtcacg gccgcgtgcg agcgctggga ggactggtgc
     2161 cggctgtggt cggagcgcgc ggcgatccac gagaagctgg gcgacgaggc ggcggcgaag
     2221 gggaagaagc tctccgcggg cgcgcactgg acgcgcgccg cggtgtgcta ccacttcggg
     2281 aaatttctgt tcgtgatcga catggagcag ctgaagcgcg cgcacgcaaa agcggtcgag
     2341 tgccgcaaca aggcgctgcc cttcctcgac ccgccggggg agcgcgtcgc gatcccgtac
     2401 gaggggaaga cgctctacgg caatctccgc aagcccgcat tttcgtcccg aagcgtggcg
     2461 aagccgccgg tcgtcgtgat gtgcatggac ctcgactccg cgaaggagga gatggacgac
     2521 tacgagaacc gcttcctcgc gcgcggcctc gcgacgctcg cgttcgacgg gccggggcag
     2581 ggcgaggccg agtacgactt cgccatctgc cccgagtacg agaagccggt gaaggccgtc
     2641 gtcgacttcg tcgagacgcg caaggacctc gacgcgaatc gcatcggcgt ctggggcgtg
     2701 tcgctcggcg gctattacgc gccgcgcgcg gccgcgttcg agaagcgctt caaggcctgc
     2761 gtcgcgctct cgggcccgta cgcgcgctcg gcctcgttcg aggggcgccc gtcgatcaac
     2821 gtcgagacct tccgcgtgcg ctcgaagagc gcgaacctgg aggaggcggg gaaggtggcg
     2881 ctgcgcatga cgctgaaagg cgccgcgaag aacatcacct gcccgatcta catcgtcgcc
     2941 gggacgaagg accgcctgac gccgcacacg cacgccgagc agctcgccgc cgaggtgtcg
     3001 ggcccgtgcg tgctctcggt gatcgagggc ggcaaccacg tggtgaacaa cctctggtac
     3061 cgctaccgcg accagaccgc cgactggatg gccgatcaat tgggcgcggg gcgctgatgc
     3121 cttgtcatcc tgagcgcgca gcgcgaagga tctgcttttc cctcgccctc gcgctgctct
     3181 ccgccttcgc gcacgcccag ccgttcccct cccgtccgct gcacctgatc gtccccttcc
     3241 ccccgggcgg ctcgaccgac atcctcgccc gcgcgctcgc gcaaaaaatg agcgagggtc
     3301 tcggccagcc ggtggtgatc gacaaccgcg gcggcgcggg cggcacgatc ggcgcggccg
     3361 aggccgcgaa ggccgcgccc gacggccaca cgatcatgat gggacacctg ggcacgctcg
     3421 ccgtcgcgcc cgccatctac aggaagctgc cgtacgaccc ggtgaggagc ttcgcgccgg
     3481 tcgcgctgat ggcgc
//
LOCUS       DATDMW010000285         2555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2329632, whole genome shotgun sequence.
ACCESSION   DATDMW010000285 DATDMW010000000
VERSION     DATDMW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329632"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..465)
                     /gene="rpsI"
                     /locus_tag="VI319_12170"
     CDS             complement(73..465)
                     /gene="rpsI"
                     /locus_tag="VI319_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY6864644.1"
                     /translation="MIGEYYYGTGRRKSAVARVFIKPGKGNFIVNDKPANEYFGRETS
                     QMVVRQPLALTNNLSNFDVTVNVVGGGEFGQSGAVRHGLTRALIEFDPALKPLLSKAG
                     LVTRDAREVERKKVGLHKARRRKQFSKR"
     gene            complement(462..896)
                     /gene="rplM"
                     /locus_tag="VI319_12175"
     CDS             complement(462..896)
                     /gene="rplM"
                     /locus_tag="VI319_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY6864645.1"
                     /translation="MTTYSAKARDIKRDWYLVDADGKVLGRLAAEIARRLRGKHKPEF
                     TPHMDTGDFIVVVNAAKLRVTGNKIEDKLYHRHSTYPGGVYTTTFGKMQKRFPTRALE
                     IAVKGMLPKGPLGYAMFRKLKVYAGPEHPHAAQQPRTLEVKA"
     gene            1077..1499
                     /locus_tag="VI319_12180"
     CDS             1077..1499
                     /locus_tag="VI319_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY6864646.1"
                     /translation="MECTVKWVGGDGMSFVAETGSGHALVMDGAPDGGGHNLAPRPME
                     TVLAGTGGCTAYDVVVILRKSGQRISGCEVRMTSERAPTDPKVFTKIHMHFVVRGKEL
                     KPSLVESAIKLSHNKYCSASAMLGKTAAISHDYEIVED"
     gene            complement(1503..2126)
                     /gene="coq7"
                     /locus_tag="VI319_12185"
     CDS             complement(1503..2126)
                     /gene="coq7"
                     /locus_tag="VI319_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEY6864647.1"
                     /translation="MTPDRIILEFDRALRTVFGVGAAERASPAQDLDEPALDPTERHH
                     AAALMRVNHCGEVCAQALYQGQALASDNDAIRRELGRAAREEADHLAWSAERIAQLGG
                     RPSLLNPLWYAGSFCVGYAAGRLGDGWNLGFLAETERQVERHLEGHLGRLSPRDERTR
                     AVIERMRDEEAGHGEKAKELGARELPAPVRRGMQLAARIMTTLAYRV"
     gene            complement(2235..>2555)
                     /locus_tag="VI319_12190"
     CDS             complement(2235..>2555)
                     /locus_tag="VI319_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864648.1"
                     /translation="QRGVGLNWEQVFGNIQALAQVARSQKATGCTESATITCDNTEST
                     SYMVGARYLLSKRTAAYVTYNKTTNKSNAAADYTAASYTANPAGFPVGADPRVWAIGL
                     MHNF"
BASE COUNT          389 a          839 c          878 g          449 t
ORIGIN      
        1 ggcgtcgcct ctcggcgacg ccataaacga aaaagccgcc cgaaggcggc ttcccgatcc
       61 cgcgattccg cctcagcgtt tcgagaactg cttgcgtcgg cgcgccttgt gcaggccgac
      121 cttcttgcgc tcgacctctc gcgcgtcgcg cgtcaccagc ccggccttgc tgagcagggg
      181 cttgagcgcc gggtcgaact cgatcagcgc gcgcgtgagg ccgtgacgca ccgcgcccga
      241 ctggccgaac tcgccgccgc cgaccacgtt taccgtgacg tcgaagttgc tcaggttgtt
      301 cgtgagcgcg agcggctgac gcacgaccat ctggctggtc tcgcggccga agtactcgtt
      361 cgccggcttg tcgttgacga tgaagttgcc cttgccgggc ttgatgaaga cgcgggcgac
      421 cgcgctcttg cgccgccccg tgccgtagta atactcgccg atcatgcctt gacctccaga
      481 gtccgcggct gctgcgccgc gtgcgggtgc tcggggcccg cgtagacctt cagcttcctg
      541 aacatcgcgt agcccagcgg ccccttgggc agcatgccct tcaccgcgat ctcgagcgcg
      601 cgcgtcggga agcgcttctg catcttgccg aaagtcgtgg tgtacacgcc gcccgggtac
      661 gtgctgtggc ggtggtacag cttgtcctcg atcttgttgc ccgttacgcg cagcttggcc
      721 gcgttgacga cgacgatgaa gtcgcccgtg tccatgtgcg gcgtgaactc gggcttgtgc
      781 ttgccgcgca gacggcgcgc gatctcggcg gcgagccgtc cgaggacttt gccgtcggcg
      841 tcgacgaggt accagtcgcg cttaatgtcg cgcgccttgg cggagtaggt ggtcataacg
      901 gttgcctgag gaagctcggg acttcgacga aagggcgtga ttttattgaa gaagtccgag
      961 ggtgtcaaac ctgattcgcc gaacctcgga cgtgggtgcg gctcgcgctc tccgcgttaa
     1021 agggttgttc gcgaatatat aatgcccgcc tcgcggcgaa aggagcgaac ccggcaatgg
     1081 aatgcaccgt gaagtgggtc ggcggcgacg gcatgagctt cgtcgcggag accgggagcg
     1141 gccacgcgct ggtaatggac ggcgcccccg acggcggggg ccacaacctg gcgccgcgcc
     1201 cgatggagac ggtgctcgcc ggcaccggcg gctgcaccgc ctacgacgtc gtggtgatcc
     1261 tgcgcaagag cgggcagcgc atcagcggct gcgaggtccg gatgacctcg gagcgtgcgc
     1321 cgacggatcc gaaggtattc acgaagatcc acatgcattt cgtcgtgcgc ggcaaggagc
     1381 tgaagccgtc gctcgtggaa agcgcgatca agctctcgca caacaagtac tgctcggcct
     1441 ccgccatgct cggcaagacg gccgcgatct cgcacgatta cgagatcgtc gaggactgac
     1501 gctcagacgc ggtacgccag cgtcgtcatg atccgggcgg cgagctgcat gccgcggcgg
     1561 accggcgcgg gaagctcgcg agcgccgagc tctttcgctt tttcgccgtg ccccgcttcc
     1621 tcgtcgcgca tgcgctcgat gacggcgcgc gtgcgctcgt cgcgcggcga aagccgcccg
     1681 aggtgtcctt cgagatgccg ctcgacttgg cgctcggtct ccgcgagaaa gccgagattc
     1741 cagccgtcgc cgagccggcc ggcggcgtaa ccgacgcaga acgagcccgc gtaccacagc
     1801 ggattgagca ggctcggacg gccgccgagc tgggcgatgc gctcggcgct ccaggcgaga
     1861 tggtcggctt cctcgcgagc cgcgcggccg agctcgcgcc ggatcgcatc gttgtcggac
     1921 gcgagcgcct gaccctggta cagcgcctgc gcgcagacct cgccgcagtg gttcactctc
     1981 atgagggcgg ccgcgtgatg ccgctcggtc gggtcgagcg ccggctcgtc gaggtcctgc
     2041 gcgggcgacg cgcgttcggc agccccgacg ccaaagacgg tccgcagcgc gcggtcgaat
     2101 tcgaggatga tgcggtcggg agtcatgtca ggccattatc gcgcacgccg gcccggtgcc
     2161 gcccgcggac gcggaaataa aaacgggcgc ccgaaggcgc ccgttcaagt cactgcttct
     2221 cggtcttttg ccgattagaa gttgtgcatc aggccgatcg cccacacgcg cgggtcggcg
     2281 ccgacgggga agcccgccgg gttggccgtg tacgacgcgg cggtgtagtc ggcggcggca
     2341 ttcgacttgt tggtcgtctt gttgtaggtc acgtaggccg cggtgcgctt cgacaacagg
     2401 tacctcgcgc cgaccatgta gctggtcgac tcggtgttgt cgcaagtgat cgtcgcgctc
     2461 tcggtacaac ccgtcgcctt ctgcgagcgc gcgacctggg caagcgcctg gatgttgccg
     2521 aacacctgct cccagttcaa gcccacgccg cgttg
//
LOCUS       DATDMW010000286         2924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1706384, whole genome shotgun sequence.
ACCESSION   DATDMW010000286 DATDMW010000000
VERSION     DATDMW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706384"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VI319_12195"
     CDS             complement(<1..250)
                     /locus_tag="VI319_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit B"
                     /protein_id="HEY6864649.1"
                     /translation="MSALPERPITILVAALGGEGGGVLADWIVAAATALEFPVQSTSI
                     PGVAQRTGATTYYIEIYPAKSTDLGGRRPVMTLTPAPGH"
     gene            complement(247..2412)
                     /locus_tag="VI319_12200"
     CDS             complement(247..2412)
                     /locus_tag="VI319_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEY6864650.1"
                     /translation="MERSFTKEVELLRLGRGQVFHGEGILAVTKALLQSGVSYVGGYQ
                     GAPISHMMDVLNDARDIMGDLGIHIETNASEAGAAAMLGASINYPIRGAVAWKSTVGT
                     NVASDALSNLASAGVRGGALIVIGEDYGEGASIIQERSHAFAMKSQIWLLDPRPELPT
                     IVRMVEKGFELSEASATPVMLELRIRACHVHGSFETRDNVRPEISTKRTLDAPDFDFG
                     RIPLPPSTYAQERVKVETRGPAAVRFVREHKLNEIFPGELASVGIVCQGGMYNAAVRA
                     LQLLGLADAFGASKIPIYALNVTYPLVPEEVAEFCAGKSAVLMVEEGQPAYLEDALHA
                     ILRKADLQTRVHGKDCLPEYDGVFPMAGEYTGEVVLAGMAKWIEKIAPQVAGLDAAAA
                     HAAHERLTAPKKRAAELLGALPPRPPGFCVGCPERPVFSAMKLLEREHGAFHISGDIG
                     CHTFSTLPPFNMGNTVLGYGLGMASNAAIEPMFSRRTITVMGDGGFWHNGLTSGIANA
                     VFNKDDGILVIMKNGYTSATGTQNIPSSPHQTEWKSGDMGIERALRGIGVKWLKSVYT
                     YRVGRMAKVLREAMTSSYRGLKVIVADAECQLERQRRIRPQVAADLAAGRRVVRTRFG
                     VDEDLCTGDHSCIRLSGCPSLTVKPSSDPLKVDPVAHVNNGCVGCGLCGEVAHAAVLC
                     PSFYRAEVVQNPRAWDRALAALRGAVIRALAPRERAPSA"
     gene            complement(2422..>2924)
                     /locus_tag="VI319_12205"
     CDS             complement(2422..>2924)
                     /locus_tag="VI319_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY6864651.1"
                     /translation="ANLMQALRGTAGISGMILFIIVGALTFSQILSFSGASNGVVESI
                     AGLGLPPMGLIAAMMLVLVFLGLFVEQVSMMLITLPIFMPLVQKMNVDTVWFGVMFLI
                     CMQLGLLLPPHGLLLMTMKGVAPPQVTMGHIFKAVLPYIAMSLLLLALVFLVPEIAVW
                     LPRVLV"
BASE COUNT          460 a          997 c         1023 g          444 t
ORIGIN      
        1 cgtggccggg cgccggcgtc agcgtcatca ccgggcgccg gccgccgagg tcggtgctct
       61 tcgccgggta gatctcgatg taataggtcg tcgcgcccgt gcgttgcgcc actccgggga
      121 tcgaggtgct ctgcacggga aactccagcg cggtcgcggc ggcgacgatc cagtccgcga
      181 gcacgccgcc gccttcgccg ccgagggcgg cgacgaggat ggtgatcgga cgctcgggca
      241 gcgcgctcat gccgaaggcg cgcgctcgcg cggggcgagc gcgcggatca cggcgccgcg
      301 cagcgcagcg agcgcgcggt cccaggcgcg cgggttctgc accacctcgg cgcggtagaa
      361 cgacgggcac agcaccgccg cgtgcgcgac ctcgccgcac aggccgcagc cgacgcagcc
      421 gttgttcacg tgcgcgaccg ggtcgacctt gagcgggtcc gagctcggct tcaccgtgag
      481 cgacggacag cccgagaggc ggatgcacga gtggtcgccc gtgcacaggt cctcgtcgac
      541 gccgaagcgc gtgcgcacca cgcgcctgcc cgcggccagg tcggccgcga cctgcgggcg
      601 gatgcgccgc tgccgctcga gctggcactc ggcgtccgcg acgatcacct tcagcccgcg
      661 gtacgagctc gtcatcgcct cgcgcagcac cttcgccatc cggccgacgc gataggtgta
      721 gaccgacttc agccacttca cgccgatgcc gcgcagcgcg cgctcgatgc ccatgtcgcc
      781 cgatttccac tcggtctggt gcggagacga cgggatgttc tgcgtgccgg tcgccgacgt
      841 gtagccgttc ttcatgatca cgaggatgcc gtcatccttg ttgaagacgg cgttcgcgat
      901 gccggaggtg aggccgttgt gccagaagcc gccgtcgccc atcacggtga tcgtgcggcg
      961 cgagaacatc ggctcgatcg ccgcgttcga cgccatgccg aggccgtagc cgagcacggt
     1021 gttgcccatg ttgaagggcg gcagcgtcga gaaggtgtgg cagccgatgt cgcccgagat
     1081 atgaaaggcg ccgtgctcgc gctcgagcag cttcatcgcc gagaacacgg ggcgctcggg
     1141 gcagccgacg cagaagccgg gcgggcgggg cggcagggcc ccgagcagct cggcggcgcg
     1201 ctttttcggg gcggtcaggc gctcgtgcgc ggcgtgcgcc gcggcggcgt cgagtcccgc
     1261 gacctgcggc gcgatcttct cgatccactt cgccatgccg gcgaggacta cttcgccggt
     1321 gtactcgccg gccatgggga agaccccatc gtattcgggg aggcagtcct tgccgtgaac
     1381 cctggtttgc agatcggcct tgcgcaggat cgcgtgcagc gcgtcctcga ggtacgccgg
     1441 ctggccttcc tcgaccatca gcaccgcgct cttgccggcg cagaactcgg cgacttcctc
     1501 cgggaccagc gggtaggtga cgttcagcgc gtagatcggg atcttcgacg ccccgaacgc
     1561 gtcggcgaga ccgagcagct gcagcgcgcg caccgcggcg ttgtacatcc cgccctggca
     1621 gacgatgccg acgctcgcga gctcgcccgg gaagatctcg ttcagcttgt gctcgcgcac
     1681 gaagcgcacc gcggcgggcc cccttgtctc gaccttcacg cgctcctgcg cgtaggtcga
     1741 gggcggcagc ggtatgcggc cgaagtcgaa gtcgggcgcg tccagcgtcc gcttcgtcga
     1801 gatttcgggc cggacgttgt cccgggtctc gaacgacccg tgcacgtggc aggcgcggat
     1861 gcgcagctcg agcatcaccg gcgtcgccga cgcctccgag agctcgaagc ccttctcgac
     1921 catccggacg atggtcggca gctcgggccg cgggtcgagc agccagatct gcgacttcat
     1981 ggcgaaggcg tggctgcgct cctggatgat cgaggcgccc tcgccgtagt cctcgccgat
     2041 gacgatcagc gcgccgccgc gcacgcccgc ggaggcgagg ttcgacagcg cgtccgaggc
     2101 gacgttggtg ccgacggtcg acttccacgc gaccgcgccg cggatcgggt agttgatcga
     2161 ggcgccgagc atcgccgcgg cgcccgcttc cgaggcgttg gtctcgatgt ggatgccgag
     2221 gtcgcccatg atgtcgcggg cgtcgttcag cacgtccatc atgtgcgaga tcggcgcgcc
     2281 ctggtagccg ccgacgtacg agacgcccga ctgcagcagc gccttggtga cggcgaggat
     2341 gccctcgccg tggaagacct ggccgcggcc gaggcggagg agctcgacct ccttcgtgaa
     2401 agagcgttcc atgccgctat tctagaccag caccctcggc agccagacgg cgatttccgg
     2461 gacgaggaag acgagcgcga gcagcagcag gctcatcgcg atgtaaggca gcacggcctt
     2521 gaagatatgg cccatcgtga cctgcggagg cgccactcct ttcatcgtca tcaataacag
     2581 gccgtggggg ggtagcaaga gcccgagctg catgcagatc aggaacatga cgccgaacca
     2641 gaccgtgtcg acgttcatct tctgcaccag cggcatgaag atcggcaggg tgatcagcat
     2701 catgctcacc tgctcgacga acagtcccag gaacacgagc accagcatca tcgccgcgat
     2761 caatcccatc ggcggcaggc ccagtcccgc gatcgactcg accacgccgt tgctcgcgcc
     2821 ggagaacgac aggatctgcg agaaggtgag cgcgccgacg atgatgaaga ggatcatccc
     2881 cgagatgccg gccgtgccgc gcagcgcctg catcaggttc gcga
//
LOCUS       DATDMW010000287         5046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1332732, whole genome shotgun sequence.
ACCESSION   DATDMW010000287 DATDMW010000000
VERSION     DATDMW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332732"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VI319_12210"
     CDS             complement(<1..1199)
                     /locus_tag="VI319_12210"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6864652.1"
                     /translation="MLFGCASSVPRPDAIGRGDYDAVRRYASALIQEEMERAGIAGMS
                     IALVDDQKVVWAEGFGYADREGQLPATADTIYRIGSITKLFTATAAMQLAEAGKIDID
                     QPVSAVLPGFSMHSRFPGAGPVTPRLLMTHHSGLPSDILHGFTQAHPASLAETEQELR
                     NTWLAYPPGELWSYSNVGFSLLGRIVEVAAGEPYAEHLDRAIFRPLGMEHSWISPGIS
                     TRPGSAKGYRNGHTQDDFILRDVPAGGMNSTVRDLSKFLEMVFADGRAGGRQIIRPET
                     LHEMMRVQNAGNPLDLTYQIGLAWALGPVGDLVLPEGELIAQHTGGTITFHSQLIALP
                     KTKLGIVVLSNSQAARTVVGKVAHAVLKAAYEAKTGIRPPERHERTADRRPLPPELVR
                     AWEGEYAT"
     gene            complement(1255..3234)
                     /locus_tag="VI319_12215"
     CDS             complement(1255..3234)
                     /locus_tag="VI319_12215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY6864653.1"
                     /translation="MDFFEQQHRARRHTALMVVLFVAAVAAIVAVLDGVAAVVYAVLT
                     VAEAPTLSGLLARTPRGVYYATSAVVLGAIAIATGKRLWELSEGGAAVADLVDARRVE
                     RDTQDAGERRLLNIVEEMALASGIPAPLVYLMDRQTSINAFAAGFSPYEASVIVTRGA
                     LERLDRDELQGVVAHEFSHILNGDMRLNIRLLGVISGIVVIGTIGGYLMLPPDPENRG
                     DRTVNVQIQFFALGLVLWLIGSIGVLAGRIIKAVIAREREFLADASAVQFTRNPDGIA
                     GALFKVSERGSVVATMHAEELSHMCIAEPVNLYFEFPWLRSHPPIDNRIERVLGPGAK
                     YILRDRARRAEQAALAAKPSPVATELGSPLYARALEPIRTTASAVLASVGRPSEDHVD
                     YAQRLLASLPPDLRGAAQSADGARALVYALLLGDGEMRAAQLRVIADAAIGSRASALA
                     DALSRLDARIVMPLLGLVIPTLKVLASQERETVLQTAQRLAEADHKISLHEFALLTVL
                     RRRLGADAKGPGPVRYREVREVSKEATLVLSLLAQAGGGAEPALLKGMAALGLGSGMT
                     IERSALGLAAVESALRELALLAPLRKPLLIKACLDVVMADGRIAAAEGELMRAICAAL
                     DTPLPPILDAAPRQAVAAAQTNTRGEYAAEERHAI"
     gene            complement(3256..3843)
                     /locus_tag="VI319_12220"
     CDS             complement(3256..3843)
                     /locus_tag="VI319_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY6864654.1"
                     /translation="MGWIVLGVLAALAVWLIVIYNGLVGLRNRFKNAYSQIDVQIKRR
                     HDLIPNLVETAKAYMAHERQTLEAVIAARNSAAGAAQRAAANPADPDAIRAMQAAEAG
                     LTGALGRLFAVSESYPELKANQTMMQLSEELTSTENRIAFARQAYNDSVMEYNTGIES
                     FPNNFVKGFGSFPPATLLEATESPEEKKPVKVSFG"
     gene            3933..4910
                     /locus_tag="VI319_12225"
     CDS             3933..4910
                     /locus_tag="VI319_12225"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864655.1"
                     /translation="MIERLSVLLIAAVVIFPSSGGAQPESWPGRPVHVIATSPPGGSV
                     DLLARLAAEEFARSFGEPFVVENRPGANGNIGAEIVLKAPADGHMFFVAPTGPFAINA
                     QLMAKMPFDPEKDLLPVALLGVSPLLLVVHPSVPATNVRELIAWMKAQGGRVDYASQA
                     VASTGHLAMELFKAMTGVEATHIPYKGSAAQATADLLAGRVSVSFVNVSATIPYIRTG
                     QLRALAVAERRRLAVAPEIPTVDESGLPGYEATTWIGLGTRAGTPREIVARLNEAAAR
                     GLARPETAQRLARVGVEPRTMTPEQFTAFVRAETAKWGAIIRRSGARVE"
BASE COUNT          736 a         1772 c         1784 g          754 t
ORIGIN      
        1 gtggcgtact cgccctccca ggcgcgcacc agctcgggcg ggagcggccg gcggtcggcg
       61 gtgcgctcgt ggcgctcggg cggccggatg ccggtcttcg cttcgtacgc cgccttgagc
      121 acggcgtgcg cgaccttgcc gacgacggtg cgggccgcct gcgagttcga gagcacgacg
      181 atgccgagct tggtcttcgg cagcgcgatc agctggctgt ggaacgtgat cgtcccgccc
      241 gtgtgctgcg cgatcagctc gccctcgggc agcaccaggt cgccgacggg gccgagcgcc
      301 caggcgaggc cgatctggta ggtgaggtcg agcgggtttc cggcattctg cacgcgcatc
      361 atctcgtgca gcgtctcggg ccggatgatc tggcgcccgc ccgcgcggcc gtcggcgaac
      421 accatctcca ggaacttcga gagatcgcgc accgtcgagt tcatgccgcc cgccggcacg
      481 tcgcgcagga tgaagtcgtc ctgcgtgtgg ccgttgcgat agcccttcgc cgagccgggc
      541 cgcgtcgaga tccccgggga gatccagctg tgctccatcc cgagcgggcg gaagatcgcg
      601 cggtcgaggt gctccgcgta cggctcgccc gcggcgacct cgacgatgcg cccgaggagc
      661 gagaagccga cgttcgagta cgaccacagc tctcccggcg gatacgcgag ccaggtgttc
      721 cgcagctcct gctcggtctc cgcgagcgac gccgggtgcg cctgcgtgaa gccgtgcagg
      781 atgtcgctcg gcaggcccga gtggtgcgtc atcagcaggc gcggcgtcac cggacccgcg
      841 cccgggaacc gcgagtgcat cgagaacccg ggcagaaccg ccgacaccgg ctggtcgatg
      901 tcgatctttc cggcttcggc gagctgcatc gccgcggtgg ccgtgaacag cttcgtgatc
      961 gagccgatcc ggtagatcgt atccgccgtc gccggcaact ggccttcgcg gtccgcgtag
     1021 ccgaagccct cggcccagac gaccttctga tcgtcgacga gcgcgatgct catccccgcg
     1081 atgccggcgc gctccatctc ctcctggatc agcgcgctcg cgtagcggcg caccgcgtcg
     1141 tagtccccgc gcccgatcgc gtcgggccgc ggcacggacg aggcgcagcc gaacagcaag
     1201 gcacaaataa cgatagggag ggcgccggcg atgcggccgg acgcggtcgg acgatcagat
     1261 tgcatgtctc tcctctgcag cgtattcacc acgtgtgttc gtctgcgccg cggcgacggc
     1321 ctgtcgcggt gcggcgtcga gtatcggggg caggggcgtg tcgagcgcgg cgcagatcgc
     1381 gcgcatcagc tcgccctcgg cggcggcgat cctgccgtcg gccatcacga cgtcgaggca
     1441 cgccttgatg agcagcggct tcctgagcgg cgcgagcagc gccagctcgc gcagcgccga
     1501 ctcgaccgcg gccaggccga gcgcggagcg ctcgatcgtc atgccgctcc cgagcccgag
     1561 cgccgccatc cccttgagga gcgccggctc ggccccgccg cccgcctgcg cgagcagcga
     1621 gagcaccagc gtcgcctcct tgctcacctc gcggacttcg cgataacgga ccgggcccgg
     1681 gcccttggcg tcggcgccca gccgacgcct gagcaccgtg agcagcgcga actcgtgcag
     1741 cgagatcttg tgatccgcct cggcgagccg ctgcgcggtc tgcaggaccg tctcccgctc
     1801 ctgcgaagcg agcaccttca gggtcgggat cacgagcccg agcagcggca tcacgatgcg
     1861 cgcgtcgagc ctggaaagag cgtccgcgag cgcactcgcg cgcgagccga tcgcggcatc
     1921 cgcgataacc cggagctgcg cggcgcgcat ctcgccgtct ccgagcagca aggcgtacac
     1981 cagcgctctc gcgccgtcgg cggactgcgc cgcgccgcgc aagtcaggag gcagcgaggc
     2041 gagcagccgc tgagcgtaat cgacgtggtc ctcggacggc cgcccgacgc tcgcgagcac
     2101 ggcggaagcc gtggtgcgaa tgggctccag tgcgcgggca tagagcgggc tccccagctc
     2161 ggtcgcgacc gggctgggct tggcggccag ggcggcctgc tccgcgcgcc gggcgcgatc
     2221 gcgcagaatg tatttcgcgc cgggaccgag gacgcgctcg atgcgattgt cgatcggcgg
     2281 gtgcgaccgc agccacggga actcgaagta gaggttgacg ggctccgcga tgcacatgtg
     2341 cgagagctcc tcggcgtgca tcgtcgccac gacgctcccg cgctcggaca ccttgaacag
     2401 cgctcctgcg atcccgtccg ggttgcgagt gaactggacc gccgaggcgt cagccaggaa
     2461 ctcgcgctcc cgcgcgatga ccgctttgat gatgcggccc gcgagcacgc cgattgaccc
     2521 gatcagccac agcaccaggc cgagggcgaa gaactggatc tggacattga ccgtgcgatc
     2581 cccgcggttc tccgggtcgg gaggcagcat gaggtagccg ccgatcgtgc cgatgaccac
     2641 gatgccggag atcacgccga gcaggcggat gttgagcctc atgtcgccgt tcaggatgtg
     2701 gctgaactcg tgcgcgacca cgccctgcag ctcgtcgcgg tccaggcgct cgagcgcgcc
     2761 gcgcgtcacg atgacgctcg cctcgtaggg cgaaaagccg gccgcgaagg cgttgatcga
     2821 agtctgccgg tccatgaggt agacgagcgg agccgggatg cccgacgcga gcgccatctc
     2881 ctcgacgatg ttcagcagcc gccgctcgcc ggcgtcctgc gtatcgcgct cgacgcggcg
     2941 cgcgtcgacc aggtcggcga ccgccgcgcc tccctcggaa agctcccaca gccgcttgcc
     3001 ggtcgcgatc gcgatcgccc cgaggaccac ggcgctcgtc gcgtagtaca cgccccgggg
     3061 cgtccgggcg agcaatcccg agagcgtagg cgcctcggcc acggtgagca ccgcgtagac
     3121 gaccgccgcc acgccgtcga gcaccgccac gatcgccgcg accgccgcga cgaacagcac
     3181 caccatcagc gccgtgtggc gccgcgcccg gtgctgttgc tcgaagaaat ccaagggacg
     3241 acgcttcggc cggcgctagc cgaaggacac cttgacgggc ttcttctcct cgggcgactc
     3301 ggtcgcctcg agcagcgtcg ccggggggaa gctgccgaag cccttgacga agttgttggg
     3361 gaagctctcg atgccggtgt tgtattccat caccgagtcg ttgtaggcct ggcgcgcgaa
     3421 cgcgatgcgg ttctcggtgc tggtcagctc ctccgagagc tgcatcatcg tctggttggc
     3481 cttgagctcg ggatacgact cggagaccgc gaacagccgc ccgagcgcgc cggtgaggcc
     3541 cgcttcggcg gcctgcatgg cgcggatcgc gtcggggtct gcggggttcg cggcggcgcg
     3601 ctgcgcggcg ccggccgcgc tgttgcgcgc ggcgatgacg gcttcgagcg tctggcgctc
     3661 gtgcgccatg taggccttcg ccgtctcgac caggttcggg atcaggtcgt ggcggcgctt
     3721 gatctgaacg tcgatctggg aataggcgtt cttgaaccgg ttgcgcaggc cgaccagtcc
     3781 gttgtagatg acgatcagcc ataccgccag cgcggcgagc acgccgagca cgatccagcc
     3841 catgtcccct cccttcttat attgtggaca cgcgagcctc gatgataaac caccggggac
     3901 gcgctgagtg cgctcaagta tcattgcggc gcatgatcga gcgcctgtcc gtccttctga
     3961 tcgcggccgt cgtgattttt ccctcgtcgg ggggcgccca gcccgaaagt tggcccggcc
     4021 gtcccgtgca cgtgatcgcg accagcccgc ccggcggctc ggtcgacctg ctcgcgaggc
     4081 tcgccgccga ggagttcgcg cgcagcttcg gcgagccctt cgtcgtcgag aaccgtcccg
     4141 gcgcgaacgg caatatcggc gccgagatcg tgctcaaggc gccggccgac gggcacatgt
     4201 tcttcgtcgc gccgacgggt ccgttcgcca tcaacgcgca gctcatggcg aagatgccgt
     4261 tcgacccgga gaaggacctc ttgccggtgg cgctgctcgg cgtgtcgccg ctgctgctcg
     4321 tcgtgcatcc gtcggtgccg gcgacgaacg tgcgcgagct gatcgcctgg atgaaggcgc
     4381 agggcgggcg cgtcgactat gcctcgcagg cggtcgcctc caccggccac ctcgcgatgg
     4441 agctgttcaa ggcgatgacc ggcgtcgagg cgacgcacat cccgtacaag ggctcggccg
     4501 cgcaggcgac ggccgacctg ctcgcggggc gcgtctcggt gagcttcgtc aacgtcagcg
     4561 cgaccattcc gtacatccgc acgggacagc tgcgcgcgct cgcggtcgcc gagcggcggc
     4621 ggctcgccgt cgccccggag attccgaccg tggacgagtc cggccttccc ggctacgagg
     4681 cgacgacctg gatcggcctc ggaacgcgcg ccggcacgcc gcgcgaaatc gtcgctcggc
     4741 tcaacgaggc ggcggcgcgc ggtctcgcgc ggccggaaac cgcgcagcga ctcgcgcgtg
     4801 tcggcgtcga gccgaggacg atgacgcccg agcagttcac cgccttcgtg cgcgccgaga
     4861 ccgccaagtg gggcgccatc atccggcgct ccggggcgcg ggtggaatga tggcgaacgg
     4921 gctggcgcgc cttcccgccg tcgagctcgc gcgcctgatg cgcgagcggc aggtgagccc
     4981 ggtcgaggtg ctcgaggcgc acctcgcgca gatcgagaag cacaacggcc ggatcaacgc
     5041 cttctg
//
LOCUS       DATDMW010000288         4939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1955584, whole genome shotgun sequence.
ACCESSION   DATDMW010000288 DATDMW010000000
VERSION     DATDMW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955584"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VI319_12230"
     CDS             <1..357
                     /locus_tag="VI319_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864656.1"
                     /translation="RDVRPAAWCAHLEAGGASATWRLLLEFSTEDGRELDCILGALGE
                     HIPAEFERVGSRLTLSSPPCAPLDAQTDLVHALAAYPRLSWARWTIWCDAGTGEAMRA
                     QGTDASDLLAIVRAPR"
     gene            complement(367..822)
                     /locus_tag="VI319_12235"
     CDS             complement(367..822)
                     /locus_tag="VI319_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6864657.1"
                     /translation="MADFGRRQGDLSARSEGGTVGRRQSDVQFDQSPDLLGVVSLDFK
                     LKVINVAWERALGYARGEMVGRPLSAFVDSDEQIPVHRLLNPNRPVAPNESIEFSLRC
                     KDGTYRCFVWTQRSVPAEQVVFIVGKDITERKKLETTQNLQSYLNRRPR"
     gene            1077..1277
                     /gene="thiS"
                     /locus_tag="VI319_12240"
     CDS             1077..1277
                     /gene="thiS"
                     /locus_tag="VI319_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY6864658.1"
                     /translation="MQLSINGEPRRLAASASVSELLAALELAGKRVAVERNGEIVPRS
                     RYEQTALADGDRLEIVVAVGGG"
     gene            1281..2102
                     /locus_tag="VI319_12245"
     CDS             1281..2102
                     /locus_tag="VI319_12245"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY6864659.1"
                     /translation="MNAPRAPQSAADPLVVAGRSYASRLLIGTGKYRDFDETRRAVEA
                     SGAEIVTVAIRRTNIGQNAGEPSLLDALPPSRFTYLPNTAGCYNADDAVRTLRLAREL
                     LDGHDLVKLEVLGDPRTLYPNVTETLAAAETLVRDGFKVMVYTSDDPIIAKRLEQIGC
                     VAIMPLASLIGSGMGILNPWNLQLILESARVPVIVDAGVGTASDAAIAMELGCDAVLM
                     NTAVAAARDPVLMAQAMRKAVEAGREAFLAGRMPKKLYTASPSSPTSGLISPKSA"
     gene            2139..2822
                     /gene="trmB"
                     /locus_tag="VI319_12250"
     CDS             2139..2822
                     /gene="trmB"
                     /locus_tag="VI319_12250"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY6864660.1"
                     /translation="MNERLSRRPIRSFVLRQGRVSNAQRRAFDALLPRYGVPYAAHVH
                     DLDAVFGRRAPRILEVGSGMGETTATIALAHPENDYLAVEVHTPGVGSLLKRIDELGL
                     GNVRVVQHDAVEVLQDMIAPESLDAVHLFFPDPWPKKRHHKRRLVQPPFAALLASRLR
                     PGGYVHAATDWEDYALQMLEVLSAVPALENTARGFAERPETRPVTKFEQRGIALGHRV
                     WDVVFRRRA"
     gene            complement(2819..4078)
                     /gene="hemW"
                     /locus_tag="VI319_12255"
     CDS             complement(2819..4078)
                     /gene="hemW"
                     /locus_tag="VI319_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY6864661.1"
                     /translation="MTTVAAIASPAGIRLQALPPLALYVHLPWCVRKCPYCDFNSHEP
                     GRAMIPIAMPGEGASAGSPPASPALPAELESEYVRALVQDLESALPEVWGRRIHSVFF
                     GGGTPSLFSAESIDALLSAFRARLALAPGCEVTLEANPGTFEAAKFRGYRDAGVNRLS
                     IGVQSFDPEALRALGRIHGAEEARRAIDIARASFDNVNLDLMYALPEQTLERARADVE
                     EAIAARTAHLSFYHLTLEPNTVFHRRPPPLPDDDSAALMQDLIAERLARSGYVNYETS
                     AWALPGRECRHNLNYWSFGDYVGIGAGAHSKVSFPERVVRSMRVKQPREYLKRASAGQ
                     AVQESRELSRADLVFEFMMNALRLAEGFAVATFLERTGLQIAAAERPLARAEQRGLIE
                     RDRERIRPTALGRRFLNDLLELFLPER"
     gene            complement(4075..4689)
                     /gene="rdgB"
                     /locus_tag="VI319_12260"
     CDS             complement(4075..4689)
                     /gene="rdgB"
                     /locus_tag="VI319_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY6864662.1"
                     /translation="MKKLVLASSNPGKLREIDALLAPLGVEVVPQSRLGIGDAEEPHA
                     TFVENALAKARHASRKSRLPALADDSGICASALGGEPGVHSARFAAEGAPAGVDRAVM
                     DARNNAKLVRLLAEHDDRRAHYVCVVVLVRHPDDPEPLIGEGRWAGEIVEAPRGDNGF
                     GYDPHFWLRDLGRTAAELDPAQKNLLSHRGKALRRLIARLQETR"
     gene            complement(4725..>4939)
                     /gene="rph"
                     /locus_tag="VI319_12265"
     CDS             complement(4725..>4939)
                     /gene="rph"
                     /locus_tag="VI319_12265"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515680.1"
                     /note="RNase PH; tRNA nucleotidyltransferase; forms
                     hexamers in Bacillus subtilis; phosphoroltic 3'-5'
                     exoribonuclease; involved in maturation of tRNA precursors
                     and removes terminal nucleotides near CCA acceptor arms of
                     mature tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY6864663.1"
                     /translation="GVPVLDLDYAEDSACDTDMNVVMTGSGGFVEVQGTAEGAPFTRA
                     QLDALLELARAGIAGLVARQKQALGV"
BASE COUNT          730 a         1730 c         1737 g          742 t
ORIGIN      
        1 cgtgacgttc ggcccgcagc gtggtgcgct cacctggagg cgggcggagc gagcgcgaca
       61 tggcggctgc tgctcgagtt ctccacggaa gacgggcgcg agctcgattg catcctcggc
      121 gcgctcggcg aacacatccc ggccgagttc gaacgcgtcg gatcccggct tacgctgtcg
      181 tcgccgccct gcgcgccgct cgatgcgcag accgatctgg tgcacgcgct cgccgcgtac
      241 ccgcggcttt cgtgggcgcg ctggacgatc tggtgcgacg ccggcacggg cgaggcgatg
      301 cgtgcccaag gcaccgacgc ctccgacctc ctggcgatag ttcgcgcgcc gcgctgaagc
      361 ggcccctcag cgcgggcgcc ggttgaggta ggactgcagg ttctgcgtcg tttcgagctt
      421 cttgcgctcg gtgatgtcct tgccgacgat gaacacgacc tgctcggcgg gcactgaacg
      481 ctgcgtccac acgaagcagc ggtaagtgcc gtctttgcag cgcagcgaga actcgatgct
      541 ctcgtttgga gcgacggggc ggttgggatt gaggagtcgg tgcaccggga tctgctcgtc
      601 ggagtcgacg aacgccgaca gcgggcgccc gaccatctcg ccgcgcgcgt agccgagtgc
      661 gcgttcccac gcgacgttga tgaccttcag cttgaagtcc agcgacacca cgccaaggag
      721 atcgggcgac tggtcgaact gaacgtccga ctgccgccgg ccgacggtgc ccccttcgga
      781 gcgtgcggaa agatcaccct gcctacgacc gaaatcagcc atggttttct tcgtcctacg
      841 gcgtcgccca ccggtcaaac gacccgcaag gcgccgccct ccctgtcgcc gttttcgcat
      901 atccgcgcaa gggatgcaat cggattgatg cttcggatgt gcaggcttac ccctgtagaa
      961 agtgacaggg tcggaagcgg ctgtatcttg gtattcatga gcttagcttg ccgcagcgga
     1021 gcgcgtgtcg cgccccgtgc taacatcgac tttccgctcg accctgtggc aacgtgatcc
     1081 agctttcgat caatggcgaa ccccggcgcc tcgccgccag cgcgagcgta agcgagctgc
     1141 tcgccgcgct ggagctcgcg ggcaagcgtg tcgcggtcga gcgcaacggc gagatcgtgc
     1201 cgcgcagccg ctatgagcag acggcgctcg cggacggcga tcgcctcgag atcgtggtcg
     1261 cggtcggcgg gggctgagcg atgaacgccc cgcgcgcgcc gcagagcgct gccgaccccc
     1321 tcgtcgtcgc cggcaggtcg tacgcgtcgc gcttgctgat cggcacgggc aagtaccgcg
     1381 atttcgacga gacgcggcgc gccgtcgagg cgagcggcgc ggagatcgtg acggtcgcga
     1441 tccgccgcac caacatcggg cagaacgccg gcgagccgtc gctgctcgac gcgctgccgc
     1501 cctcgcgctt cacctacctg ccgaacaccg ccggctgcta caacgccgac gacgcggtgc
     1561 gcaccctgcg cctggcgcgc gagctgctcg acggacacga cctggtcaag ctcgaggtgc
     1621 tcggcgaccc gaggacgctg tacccgaacg tcaccgagac gctcgctgcc gccgagacgc
     1681 tggtgcgcga cggcttcaag gtcatggtgt acacgagcga cgatccgatc atcgcgaagc
     1741 gcctggagca gatcggctgc gtcgcgatca tgccgctcgc ctcgctgatc ggatcaggca
     1801 tgggcatcct caatccgtgg aacctgcagc tcattctcga atcggcgcgc gtcccggtga
     1861 tcgtcgacgc cggcgtcgga acggcctccg acgccgcgat cgcgatggag ctcggctgcg
     1921 acgcggtgct gatgaacacc gcggtcgctg cggcccggga cccggtgctc atggcgcagg
     1981 cgatgcggaa ggcggtcgag gcgggccgcg aggccttcct cgcgggacgc atgccgaaga
     2041 agctctacac cgcatcgccc agctcgccca cgtccggact catctcgccg aagtcggcct
     2101 gaagcccgcg gccgcatccg ggacagaagg cgcccgcggt gaacgaacgg ctctccagac
     2161 gcccgatccg cagcttcgtc ctgcgccagg ggcgcgtgtc gaacgcgcag cgccgcgcct
     2221 tcgacgcgct gctgccgcgc tacggcgttc cctacgccgc gcacgtgcac gatctcgacg
     2281 ccgtgttcgg ccgccgcgcg ccgaggattc tcgaggtcgg ctccgggatg ggcgagacca
     2341 ccgcgacgat cgcgctcgcg caccccgaga acgactacct ggcggtggag gtccacacgc
     2401 ccggcgtcgg cagcctgctc aagcgcatcg acgagctcgg tctgggcaac gtgcgcgtcg
     2461 tgcagcacga cgcggtggag gtgctgcagg acatgatcgc cccggaatcg ctcgacgcgg
     2521 tgcatctctt cttccccgat ccgtggccga agaagcggca tcacaagcgc cgtctcgtgc
     2581 agccgccgtt cgcggcgctg ctcgcgtcgc gcctgcgccc cggcggctat gtgcatgccg
     2641 cgaccgactg ggaggattac gcgctgcaga tgctggaggt gctctcggcg gtgccggcac
     2701 tcgagaacac cgcgcgcggc ttcgccgagc gccccgagac gcgtccggtc acgaagttcg
     2761 agcagcgcgg gatcgcgctc ggccaccgcg tctgggacgt cgtgttccgc cggcgcgcct
     2821 agcgctcggg caggaagagc tcgagcaggt cgttcagaaa ccggcggccc agcgcggtgg
     2881 ggcggatgcg ctcccggtcg cgctcgatca gcccgcgctg ctcggcgcga gcgagcggcc
     2941 tttcagcggc tgcgatctgc agccccgtgc gctcgagaaa ggtcgccacg gcaaagccct
     3001 cggcgagcct cagcgcgttc atcatgaact cgaagacgag gtcggcgcgc gagagctcgc
     3061 ggctttcctg gaccgcctgc cctgcgctcg cgcgcttcag gtattcacgc ggctgcttga
     3121 cgcgcatgct gcgcaccacc cgctcgggaa agctgacttt gctgtgcgcg cccgcgccga
     3181 taccgacata gtcgccgaag ctccaatagt tcaggttgtg gcggcattcc cgcccgggca
     3241 gcgcccaggc agaggtctcg tagttgacgt aacccgacct ggcgagccgc tccgcgatca
     3301 ggtcctgcat cagggccgcg ctgtcgtcgt cgggaagcgg gggcggccgc cggtggaaga
     3361 cggtgttcgg ctcgagcgtc aggtgataga acgagagatg cgccgtgcgc gccgcgatcg
     3421 cctcctcgac gtcggcgcgc gcacgctcga gcgtctgctc cggcagcgcg tacatcaggt
     3481 cgagattgac gttgtcgaac gacgcgcgcg cgatgtcgat ggcgcggcgc gcctcttccg
     3541 cgccgtggat gcggccgagc gcgcgcagcg cctcgggatc gaagctctgc accccgatcg
     3601 atagccggtt cacgccggcg tcgcggtagc cgcggaactt cgccgcctcg aacgtgcccg
     3661 ggttcgcctc gagcgtgacc tcgcacccgg gcgcgagcgc gaggcgcgcg cggaacgcgg
     3721 agagcaacgc gtcgatcgac tccgccgaga acaggctcgg cgtgccgccg ccgaagaaaa
     3781 cgctgtggat gcgccggccc cacacctcgg ggagcgcgct ctcgaggtcc tgtacgagcg
     3841 cgcgcacgta ctcggactcg agctcggccg gaagcgcggg ggaggccggc ggactccccg
     3901 cggaagcccc ctcgcccggc atcgcgatcg ggatcatcgc gcgtcccggc tcgtgcgaat
     3961 tgaaatcgca ataggggcat ttgcgcacgc accagggcag atgcacgtag agcgcgagcg
     4021 gcggcagggc ctgcaggcgg atgccggcgg gcgaagcgat cgcggcgacc gtggtcaacg
     4081 ggtctcctgc agccgcgcga tcagtcgccg cagcgccttc ccgcggtggc tgagcaggtt
     4141 cttctgcgcc gggtcgagct cggcggccgt cctcccgagg tcgcgcagcc agaaatgcgg
     4201 gtcgtagccg aagccgttgt cgccgcgcgg cgcttcgacg atctcgccgg cccagcggcc
     4261 ttcgccgatc agcggttcgg ggtcgtcggg atggcgcacg agcacgacca cgcagacata
     4321 gtgcgcgcgg cggtcgtcgt gctcggcgag caggcgcacg agcttcgcgt tgttgcgggc
     4381 gtccatgacc gcgcggtcga cgcccgccgg ggcgccctcc gcggcgaagc gcgccgaatg
     4441 cacgccgggc tctccgccga gcgcgcttgc gcagatcccc gagtcgtcgg cgagcgcggg
     4501 caggcgcgac ttgcggctcg cgtgccgggc cttggcgagg gcgttctcga cgaacgtcgc
     4561 gtgcggctcc tcggcgtcgc cgatgccgag ccgcgattgc gggactactt cgacgccgag
     4621 cggcgccagc agcgcatcga tctcgcgcag cttgccgggg ttggacgagg ccaggacgag
     4681 cttcttcacc gcgacatttt atcgcggcgg cggcgaccgc ccgctcagac gcccagcgcc
     4741 tgcttctgcc gcgcgacgag ccccgcgatg ccggcgcgcg cgagctcgag cagcgcatcg
     4801 agctgcgcgc gggtgaaggg cgcgccctcg gcggtgccct gcacctcgac gaagccgccc
     4861 gagccggtca tcacgacgtt catgtcggtg tcgcaggccg aatcctcggc gtagtcgaga
     4921 tcgagcaccg gcacgccgt
//
LOCUS       DATDMW010000289         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2456536, whole genome shotgun sequence.
ACCESSION   DATDMW010000289 DATDMW010000000
VERSION     DATDMW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456536"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /gene="rpmC"
                     /locus_tag="VI319_12270"
     CDS             <1..165
                     /gene="rpmC"
                     /locus_tag="VI319_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY6864664.1"
                     /translation="HRLAERRQELFNLRFQSVTGALENSARLKLAKREIARILTVVNE
                     REAEKARAAT"
     gene            672..947
                     /gene="rpsQ"
                     /locus_tag="VI319_12275"
     CDS             672..947
                     /gene="rpsQ"
                     /locus_tag="VI319_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY6864665.1"
                     /translation="MRQPKPDHERGRRQERRGVVVSSAMDKTIVVKVDVITSHQRYKK
                     VIRRSVKFHAHDEQNTAKIGDVVRIVETRPLSKTKRWRLAEVVEAAK"
     gene            944..1312
                     /gene="rplN"
                     /locus_tag="VI319_12280"
     CDS             944..1312
                     /gene="rplN"
                     /locus_tag="VI319_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY6864666.1"
                     /translation="MIQQESRLRVADNTGAREILCIRVVGGHHRRYARVGDIIVGTVK
                     TATPQGAVKKGEVVRAVVVRTKKPFGRDDGTTIAFDENAAVIIDQQRNPRGTRIFGPV
                     ARELREKNFMKIVSLAPEVL"
     gene            1327..1683
                     /gene="rplX"
                     /locus_tag="VI319_12285"
     CDS             1327..1683
                     /gene="rplX"
                     /locus_tag="VI319_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY6864667.1"
                     /translation="MKVKKGDIVHILRGKDAGKEGRVLEALPKQGRVVVENINVVKRH
                     RRPRPIQNTTGMGGGGMTPGGIIDLAAPLPVSNVMVVCPNCGKPTRVGTAERTVKDKV
                     VRVRVCKHCGEDIDRT"
     gene            1680..2261
                     /gene="rplE"
                     /locus_tag="VI319_12290"
     CDS             1680..2261
                     /gene="rplE"
                     /locus_tag="VI319_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY6864668.1"
                     /translation="MSAVADTYTPRLKEQYENEIRPRLKEELGLDSIMQAPRIQKITL
                     NMGVGEAKTESKLLDSAIEELTTIAGQRAQIRRARKSIAQFKVREGMAVGARVTLRGA
                     RMYEFLDRLVSIALPRIRDFRGLNPRSMDGRGNFSLGIREQLIFPEINYDDIQQVRGL
                     DVTITTSAENDEHALALLRALGLPFADGDRRND"
BASE COUNT          454 a          738 c          816 g          276 t
ORIGIN      
        1 catcggctcg cggagcggcg gcaggagctg ttcaacctcc gcttccagtc ggtgacgggc
       61 gcgctcgaga actcggcgcg tctcaagctt gcgaagcgcg agatcgcgcg gatcctgacg
      121 gttgtgaacg aacgcgaggc agagaaggca agggcagcga cttgagcgag aacgagaaaa
      181 acgaagagca gatcgaggag actccggtgg agccggagtc ggccgaggag accccggtag
      241 aggcagagac tcccgcggag gagcctgaag ctgccgctga ggagcccgag gctccggctg
      301 gggagcccga ggctgccgcg gaagaggagc ccgtcacgga ggctgagcct gagcgggcgg
      361 cggaagagcc taccgcggag gccgagcctg agcccgcagc ggaagagcct gctgcggaag
      421 agccggaggc tgctgcagag gaggttcccg ccgaggagcc ggctggcgag gaacccgcag
      481 aagagccggc cgccgaggcc gcggagacga ccgaggccga agccgaggcc gctcctgccg
      541 cggacaccgc cgagggcgag gctccggctg cccagcccga gccgaagagg aagggcaagc
      601 gtctcccgcg ccgcctgcgg ccgcagaaga cgcgtcgtcg tccggtgccg agcgcagagc
      661 gcaagccgat cgtgcgccaa ccgaagcccg atcacgagcg cggccggcgc caggagcgtc
      721 ggggcgtcgt cgtctcgagc gcgatggaca agacgatcgt cgtcaaggtc gacgtgatca
      781 cctcgcacca gcggtacaag aaggtcatcc ggcggagcgt caagttccac gcgcatgacg
      841 agcagaacac cgcgaagatc ggcgacgtgg tgcggatcgt cgagacgcgc ccgctgtcca
      901 agacgaagcg ctggcggctc gccgaagtcg tggaggccgc gaagtgatcc agcaggaatc
      961 ccgcctgaga gtcgccgaca acaccggcgc gcgcgagatc ctctgcatcc gcgtcgtcgg
     1021 cggccaccat cgccgctacg cgcgggtcgg cgacatcatc gtcggtacgg tcaagaccgc
     1081 gacgcctcag ggcgccgtga agaagggcga ggtcgtgcgc gcggtggtcg tgcgcaccaa
     1141 gaagcccttc ggtcgcgacg acggcacaac gatcgccttc gacgagaacg ccgccgtgat
     1201 catcgaccag cagcgcaatc cgcgcggcac ccgcatcttc gggcctgtcg cgcgcgagct
     1261 gcgcgagaag aacttcatga agatcgtcag cctggctccg gaggtgctct gaccgtggct
     1321 cagaccatga aagtgaaaaa gggcgacatc gtgcacatcc tccgcggcaa ggacgccggc
     1381 aaggagggac gcgtgctcga ggcgctgccc aagcaggggc gcgtcgtcgt cgagaacatc
     1441 aacgtcgtca agcggcaccg gcgaccgcgc ccgatccaga acacgacggg catgggcggc
     1501 ggaggcatga ccccgggcgg gatcatcgac ctggcggctc cgctccccgt ctcgaacgtg
     1561 atggtggtgt gcccgaactg cggcaagccg acccgcgtcg gaaccgccga gcggacggtc
     1621 aaggacaagg tcgtacgcgt ccgggtctgc aagcactgcg gagaggacat cgatcggaca
     1681 tgagtgccgt agccgacacg tatacgccgc gcctgaagga gcagtacgag aacgagatcc
     1741 ggccgcgcct caaggaggag ctcggactcg actcgatcat gcaggcgccg cggatccaga
     1801 agatcacact gaacatgggc gtcggcgagg cgaagaccga gtcgaagctg ctcgactccg
     1861 cgatcgaaga gctgacgacg attgccggcc agcgcgcgca gattcgccgc gctcgtaagt
     1921 cgatcgcgca gttcaaggtg cgcgagggaa tggcggtcgg cgcgcgcgtg accctgcgcg
     1981 gcgcgcggat gtacgagttc ctcgaccggc tcgtctcgat cgcgctgccc cggatccgcg
     2041 actttcgcgg cctcaaccca cgctcgatgg acggccgcgg gaacttctcg ctcggcatcc
     2101 gagagcagct catcttcccg gagatcaact acgacgacat ccagcaggtc cgcgggctcg
     2161 acgtcacgat caccacgagc gccgagaacg acgagcacgc actcgcgttg ctgcgcgcgc
     2221 tcggcttgcc cttcgcggac ggcgacagga ggaacgacta atggccaaga cgtcgctcgt
     2281 cgtg
//
LOCUS       DATDMW010000290         4822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2456659, whole genome shotgun sequence.
ACCESSION   DATDMW010000290 DATDMW010000000
VERSION     DATDMW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456659"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="VI319_12295"
     CDS             complement(<1..1739)
                     /locus_tag="VI319_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6864669.1"
                     /translation="MAAPGAAPADALADAERHRADLHQSAEAIVDPLGLAAPIAHAAL
                     AWWLHPLEAAERSRRFAVELEALVLHALARFAGSKAPDLVAADPDDARFGAPEWTAQA
                     GPDVLKQWYLFWTRQIQDAIYATPGLSPKEKRRAAFWSRQWLNAAAPSNFLATNPVAL
                     RKAAETRGESLARGLGVFLKDLAAGTIRMTEPEDFAVGGNLATTPGAVVFRNRLIELI
                     HYRPAAGRVRPVPVVIVPPWINKFYVLDLDARKSLVRYLVEHGFDTYIVSWKNPGEAT
                     GPASFDDYLTEGVDEAMRVARAVSGSEKTHAVGYCIGGTLLSSYMAWLARRHPREKMP
                     VAHWTLFASLVDFQSPGDIEVFIDEGSVDYLCNLMRLRGHLDGAEMAMTFRLLRANPL
                     IWHYVVHGWLYGEPPPASDVLYWNMDTTRMPAAMHEFYLREMYLANKLVRPDALTIAG
                     EPIDLARIHQDLYAVSSEDDHITPWRQVFRINGHVTGHKRFVLSSSGHILGIVNPPVT
                     PPKRHYRAGPAHRGQAADAWLATVEQRAGSWWEDWSAWLAERCGEPAAPPPLFTEQFQ
                     KLADAPGTYVLER"
     gene            1980..3797
                     /locus_tag="VI319_12300"
     CDS             1980..3797
                     /locus_tag="VI319_12300"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY6864670.1"
                     /translation="MLRKQWSDPLYSVCWVVLCVSLPLALAVAMPGGPAPGLIGGFTP
                     LHGLAETFAIVVAMLVFGVTWNAYSTERSGNLVILACALFSAALVDVGDMFLTDDFSS
                     RPLAPTDLPVDFTLPSNIIAAAGLLIVSLRRPLPLTGPFARHLILAASLLIVVPVYVA
                     TFLSAPTLHLLFAGGGDVVRYEPQAEALIAAALLVPAVRFYLQARRSRSEEAAGIFGA
                     TCMLVFAQIGFALQSASEAGFVLMGHGYKIAAYAFIYRAVFIVSVKEPFERVRAAERQ
                     NRLANELLERVFESVHVLIAYIDRDMNFVRVNSAYASADGRPPEFFAGKNHFALYPHA
                     ENEAIFRRVVDTGEPYSAYARPFAYPERPERGTTYWDWHIEPVKDAEGRVQALVLSLL
                     DVTQRVRHEEILGASLEDSIQAIAATVESRDPYTAGHQRRVAALATAIARELGMPEER
                     IRGLHLAATIHDLGKIHIPAEILTKPGRLSPIEFEMMKLHPQAGHDIVKDIKFPWPIA
                     QIIVQHHERMDGSGYPKGLRGEAILPEARIIAVADVAESMMSHRPYRPALGLDAALTE
                     VIRGRGTAFDPAVVDAFVRLIRDAKFSFDTAEKSHRRSS"
     gene            3794..4534
                     /gene="fabG"
                     /locus_tag="VI319_12305"
     CDS             3794..4534
                     /gene="fabG"
                     /locus_tag="VI319_12305"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY6864671.1"
                     /translation="MNLNGKVAIITGGARGIGHATAVKFASLGARVAVCDINPDLAQQ
                     TLAEIRSGGGEARGYRVDVTDKQEIAGMVRSVMDQWGRVDALVNNAGIVMDAQLKKMT
                     DEQFDKVIDINLKGVYNCTRAVVDIMLAQKSGVILTTSSIVGLYGNFGQTNYAASKFA
                     VIGMTKTWARELGRSGIRANAVCPGFIATPILAKMPEEILKQMAAKVPCGRLGTSEEV
                     ASVFAFLASDEASYVNGAVISVDGGMTL"
     gene            complement(4535..>4822)
                     /locus_tag="VI319_12310"
     CDS             complement(4535..>4822)
                     /locus_tag="VI319_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6864672.1"
                     /translation="ELGVARYVMNPDGETCEFAIVVADEWQGKGLARRMLEMLIDVAR
                     SRGLKAIIGHILSMNHPMIKLSEKLGFTIADGVDDPTTKRATLALERAIGR"
BASE COUNT          748 a         1677 c         1643 g          754 t
ORIGIN      
        1 tagcgctcga gcacgtaagt cccgggcgcg tcggcgagct tctggaattg ctccgtgaag
       61 aggggcggcg gcgcggccgg ctcgccgcag cgctcggcga gccacgcgct ccagtcctcc
      121 caccagctgc ccgcacgctg ctcgacggtg gcgagccagg cgtcggcggc ctggccgcga
      181 tgcgcgggcc ccgcgcgata gtggcgcttg ggcggcgtca ccggcgggtt gacgatgccg
      241 aggatgtggc ccgacgagga cagcacgaag cgcttgtggc cggtgacgtg gccgttgatg
      301 cggaacacct gccgccacgg cgtgatgtgg tcgtcctcgg aggacacggc gtagaggtcc
      361 tggtggatgc gcgcgaggtc gatcggctcg cctgcgatcg tgagcgcgtc ggggcgcacg
      421 agcttgttcg cgaggtacat ctcgcgcagg tagaactcgt gcatcgccgc cggcatgcgc
      481 gtcgtgtcca tgttccagta gagcacgtcg ctcgccggcg gcggctcgcc gtacagccat
      541 ccgtggacca cgtaatgcca gatgagcgga ttcgcacgca gcagccggaa ggtcatcgcc
      601 atctcggcgc cgtccaggtg gccgcgcagg cgcatcaggt tgcacaggta gtcgacgctg
      661 ccctcgtcga tgaagacttc gatgtcgccc ggcgactgga agtcgacgag cgacgcgaac
      721 agcgtccagt gcgcgaccgg catcttctcg cgcggatggc ggcgcgcgag ccacgccatg
      781 tagctggaga gcagcgtccc gccgatgcag tagcccacgg cgtgcgtctt ctccgagccg
      841 ctcaccgcgc gcgcgacgcg catcgcctcg tccacgcctt cggtcaggta gtcgtcgaac
      901 gacgcgggac cggtcgcttc gccggggttc ttccagctga cgatataggt gtcgaacccg
      961 tgctcgacaa ggtacctgac caggctcttc ctcgcgtcga ggtcgagcac gtagaacttg
     1021 ttgatccacg gcggaacgat cacgaccggc acgggccgca cgcgcccggc agccggtcga
     1081 tagtggatca gctcgatcag ccggttgcgg aacacgaccg ccccgggcgt cgtcgcgaga
     1141 ttgccgccga ccgcgaaatc ctccggctcg gtcatccgga tcgtgcccgc cgcgagatcc
     1201 ttcaggaaca cgccgagtcc gcgcgccagg ctctcgcctc gggtctccgc cgccttcctg
     1261 agcgccaccg ggttggtcgc gaggaagttg ctcggcgccg cggcattgag ccactgccgc
     1321 gaccagaacg cggcgcgacg cttctccttc ggcgacagtc cgggcgtcgc gtagatcgcg
     1381 tcctggatct ggcgtgtcca gaagagatac cactgcttga gcacgtccgg gccggcctgc
     1441 gcggtccact cgggcgcccc gaagcgcgca tcgtcgggat cggcggcgac caagtcgggc
     1501 gccttgctgc ccgcgaagcg cgcgagcgcg tgcagcacca gggcctccag ctcgacggcg
     1561 aaccggcgcg agcgctcggc cgcctccagc ggatgcagcc accacgcgag cgcggcgtgc
     1621 gcgatcggcg ccgcgagccc gagcggatcg acgatcgcct cggcgctctg gtgcagatcc
     1681 gcgcgatgcc gttcggcgtc ggcgagcgcg tccgccggcg cggcacccgg cgcggccaag
     1741 gcgccagtct ccattgagcc catgcccgta cgatgccgct cccgcgcacc cgggctttga
     1801 cctgggtcaa tcggacggcc ggcgccctga acagcggtcc gggcgcgtcg aaattgaccc
     1861 gggtcaagtg tcgacggcgc ggaaacgcta gtctgcctcg aatggcagac gagcgcaccg
     1921 tgcaagctca tccgctccgg acgcgcgcgc ccgcaccggc ccggatcgcg ctgacgcgca
     1981 tgctccgaaa acagtggtcc gatccgctct actccgtctg ctgggtggtg ctttgcgtgt
     2041 cgctgccgct ggcgctcgcc gtggcgatgc cgggcggccc cgcgccgggg ctgatcggcg
     2101 ggttcacgcc gctgcacggc ctcgcggaga cattcgcgat cgtcgtcgcg atgctcgtct
     2161 tcggcgtgac ctggaacgcc tacagcaccg agcgctcggg gaacctggtg atcctcgcgt
     2221 gcgcgctgtt ctcggccgcc ctggtggatg tcggcgacat gttcctgacg gacgatttct
     2281 cgagccggcc gctcgcgccg acggatctgc cggtcgattt cacgctgcct tccaacatca
     2341 tcgccgcggc cggactgctg atcgtctcgc tgcgccggcc gctgccgctc accggcccct
     2401 tcgcgcgcca cctgatactc gcggcgagcc tgctcatcgt cgtgcccgtc tacgtcgcga
     2461 ccttcctgtc cgcgccgacc ctgcatctcc tgttcgcggg cggcggcgat gtcgtgcgct
     2521 acgagccgca ggccgaagcg ctgatcgcgg cggcgctgct ggtgccggcg gtgcgtttct
     2581 acctgcaggc gcggcgctcc aggtccgagg aggcagcggg catcttcggc gcgacgtgca
     2641 tgctcgtgtt cgcgcagatc gggttcgcgc tgcaatcggc gtccgaagcg ggcttcgttc
     2701 tcatgggcca cggctacaag atcgccgcgt acgccttcat ctatcgcgcc gtgttcatcg
     2761 tcagcgtgaa ggagccgttc gagcgggtgc gcgccgccga gcgacagaac cggctggcga
     2821 acgagctgct cgagcgggtg ttcgagagcg tccacgtcct gatcgcctat atcgaccgag
     2881 acatgaattt cgtgcgcgtg aacagcgcct acgcgagcgc ggacgggcgt ccgcccgagt
     2941 tcttcgcggg caagaaccac ttcgcgctct atccgcacgc ggagaacgag gcgatcttcc
     3001 ggcgcgtggt cgacaccggc gaaccctact ccgcatatgc caggcccttc gcttacccgg
     3061 agcgacccga gcgcggcacg acctactggg actggcacat cgagcccgtg aaggacgccg
     3121 agggccgcgt gcaggcgctc gtgctgagcc tgctcgatgt gacgcagcgc gtgcgccacg
     3181 aggagatcct cggggcgagc ctcgaggact cgatccaggc gatcgccgcg acggtcgagt
     3241 cgcgcgaccc gtacaccgcc ggccaccagc gccgcgtcgc ggcgctcgcc accgcgatcg
     3301 cgcgcgagct gggaatgccc gaggagcgga tccgcgggct gcacctggcg gccacgatcc
     3361 acgatctcgg aaagatccat atcccggcgg agatcctgac caagcccggg cgcctgagcc
     3421 cgatcgagtt cgagatgatg aagctgcatc ctcaggcggg ccacgacatc gtcaaggaca
     3481 tcaaatttcc ctggccgatc gcgcagatca tcgtgcagca ccacgaacgc atggacggca
     3541 gcggctatcc caaggggctg cgcggcgaag cgatactgcc cgaggccagg atcatcgccg
     3601 tcgccgacgt cgcggagtcc atgatgtcgc accgcccgta ccgccccgcc ctgggcctgg
     3661 acgcggcgct gaccgaagtc atccgcgggc gcgggaccgc cttcgatccc gcggtcgtcg
     3721 acgctttcgt tcggctgatc cgcgacgcaa aattcagctt cgataccgcc gagaagtccc
     3781 acaggagatc gtcatgaacc tcaacggcaa ggtcgccatc atcaccggcg gcgcgcgagg
     3841 catcggccat gccaccgcgg tcaagttcgc atcgctcggc gcgcgcgtcg ccgtgtgcga
     3901 catcaacccc gacctcgcgc agcagacgct cgccgagatc cgctcgggcg gcggcgaggc
     3961 gcggggctac cgcgtcgatg tcaccgacaa gcaggagatc gcgggcatgg tgaggagcgt
     4021 catggaccag tggggccgcg tcgatgcgct ggtcaacaac gccggcatcg tcatggacgc
     4081 gcagctcaag aagatgaccg acgagcagtt cgacaaggtg atcgacatca acctgaaggg
     4141 cgtctacaac tgcacgcgcg ccgtcgtcga catcatgctc gcgcagaagt cgggcgtgat
     4201 cctcaccacg tcgtcgatcg tggggctgta cggcaacttc gggcagacga actacgcggc
     4261 gagcaagttc gccgtcatcg gcatgaccaa gacctgggcg cgcgagctcg gacgcagcgg
     4321 catccgcgcc aacgcggtgt gcccgggctt catcgccacg cccatcctcg ccaagatgcc
     4381 ggaggagatc ctgaagcaga tggcggccaa ggtgccctgc gggcgcctgg ggacctccga
     4441 ggaggtcgcc agcgtgttcg ccttcctcgc ctcggacgag gcgagctacg tcaacggcgc
     4501 ggtgatcagc gtcgacggcg gcatgacgct gtagtcagcg gccgattgcc ctctcgagcg
     4561 cgagcgtcgc ccgcttggtg gtcgggtcgt cgacgccgtc ggcgatcgtg aacccgagct
     4621 tctccgagag cttgatcatc gggtggttca tcgacaggat gtggccgatg atcgccttca
     4681 gcccgcgcga gcgagcgacg tcgatcagca tctcgagcat gcgacgcgcg aggcccttgc
     4741 cctgccattc gtcggccacc acgatggcga actcgcaggt ctcgccgtcc ggattcatga
     4801 cgtagcgcgc gaccccgagc tc
//
LOCUS       DATDMW010000291         3259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_462569, whole genome shotgun sequence.
ACCESSION   DATDMW010000291 DATDMW010000000
VERSION     DATDMW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462569"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VI319_12315"
     CDS             <1..727
                     /locus_tag="VI319_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY6864673.1"
                     /translation="SLAAVLLDVVKQRSYTHVLAPATGFGKNVMPRVAAKLDVAQVSD
                     VTSIESPDTFVRPIYAGNALATVPSSDKVKVVTVRTTGFDAAAAAGGSAAIENAPAVA
                     DPGLSSFVGRDVTKSDRPELTAAKIIVSGGRGMGSGENFKLLEPLADKLGAAMGASRA
                     AVDAGFVPNDWQVGQTGKIVAPDLYIAVGISGAIQHLAGMKDSRVIVAINKDEEAPIF
                     QVADYGIVGDLFQVVPELVKELG"
     gene            724..1725
                     /locus_tag="VI319_12320"
     CDS             724..1725
                     /locus_tag="VI319_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864674.1"
                     /translation="MRRALPLRAALVANAALVATAALVASAALALCGAAQGATFSYDA
                     DVDRFGAAIAAPSGRRDGVRQAREYCGSTFGDLGWDAYSAYGQWVRRHAGFLQLTIAM
                     QRALSSAASQDDSGHWQRVVEVDVPKVAADLAAGLPHQVSSPESEADKRKACEETVAA
                     LHARKLDLDAVEPAIASYLREMAPKFRIALPAPGEDPSLGAPEARRDAQALLGKWTTE
                     KIRFYLADGRFSEDDTTCTFDFTGRQVTSECMVQGRLVRMVSSYEVKAPGRYDSTVVE
                     NASYPQMVGAKDTTQFRVEKGKLVQSSYLPIYSADPMRPVEIEATLAPSVLSGRRSQ"
     gene            1722..>3259
                     /locus_tag="VI319_12325"
     CDS             1722..>3259
                     /locus_tag="VI319_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6864675.1"
                     /translation="MSEYKAPVRDMRFVLNELAGLADVARLPGCEDVTAELVDQILEE
                     SARFSGEVLAPLNQPGDEEGSHWADGKVTTPKGFKEAYQAFIGGGWNALQFPTEFGGQ
                     GLPKLVATPVMEMWKSANLSFSLCPLLTTGAVEALLLRGSPQLKKTYLPKMVQGTWTG
                     TMNLTEPQAGTDLALLRTKAVRAGDHYKISGQKIFITYGEHDLAENIVHLVLARTPDA
                     PEGVRGISLFVVPKLVPKPDGTPGERNKVACASIEHKLGIHASPTAVMVYDGATGYLV
                     GEENRGLEYMFIMMNAARFAVGIEGISISERAYQQALAYAKERIQSRDLSGDAKAVPI
                     IRHPDVRRMLMHMKAMTEAMRALAYVVAAGLDRAHKHPEKEERMRAQAFVDLMIPVVK
                     GWSTETGVEVASTGIQVHGGVGFIEETGAAQHYRDARITTIYEGTTGIQANDLIGRKI
                     AREGGATAKEVVKMMRRVEAELGSEDEDSNAIRVAFSAAITALEEAVSWICINYTADI
                     KAAHA"
BASE COUNT          551 a         1068 c         1186 g          454 t
ORIGIN      
        1 atcgctggcc gcggtgctgc tcgacgtcgt gaagcagcgc agttacacgc acgtgctcgc
       61 gcccgcgaca gggttcggca agaacgtaat gcctcgtgtc gcggccaagc tcgatgtggc
      121 gcaggtttcc gacgtcacct ccatcgagtc acccgacacg ttcgtgcggc cgatctacgc
      181 gggcaacgcg ctcgcgacgg tgccgtcttc ggacaaggtc aaggtggtca ccgtgcgcac
      241 gacgggcttc gacgcggcag cggcggcggg cggctcggcg gcgatcgaga acgcgcccgc
      301 ggtcgccgac ccggggctct cgtccttcgt cggccgcgac gtcaccaagt ccgaccggcc
      361 cgagctgacc gcggcgaaga tcatcgtctc gggcggccgc ggcatgggct cgggggagaa
      421 cttcaagctg ctcgagccgc tcgccgacaa gctcggcgcg gcgatgggcg cgtcgcgcgc
      481 ggcggtggac gcgggcttcg tgccgaacga ctggcaggtc gggcagacgg ggaagatcgt
      541 cgcgccggat ctgtacatcg cggtcgggat ctcgggcgcg atccagcacc tcgccgggat
      601 gaaggacagc cgcgtgatcg tcgcgatcaa caaggacgag gaggcgccga tcttccaggt
      661 cgccgactac ggcatcgtgg gcgacctgtt ccaggtcgtg cccgagctgg tgaaggagct
      721 ggggtgaggc gcgcgctgcc gctgcgcgca gcgctcgtgg cgaatgcagc gctcgtggcg
      781 accgcagcac tcgtggcgag cgcggcgctc gcgctttgcg gcgcggcgca gggcgcgacg
      841 ttctcctacg acgccgacgt cgaccgcttc ggcgccgcga tcgccgcgcc gtcggggcgc
      901 cgcgacggcg tgcgccaggc gagggagtat tgcggctcga ccttcggcga tctcggctgg
      961 gacgcgtact cggcgtacgg gcagtgggtg cggcgccatg cgggattcct gcagctcacg
     1021 atcgcgatgc agcgcgcgct ctcgagcgcg gcgtcgcagg acgattccgg ccactggcag
     1081 cgcgtggtcg aggtcgacgt gccgaaagtc gccgccgacc tcgcggccgg actgccgcac
     1141 caggtctcga gcccggagtc ggaggcggac aagcgcaagg cgtgcgagga gacggtcgcg
     1201 gcgctgcacg cgcgcaagct cgatctcgac gcggtggagc ccgcgatcgc gtcgtatctg
     1261 cgcgagatgg cgccgaagtt ccgcatcgcg ctgccggcgc cgggcgagga tccgtcgctg
     1321 ggggcccccg aggcgcggcg cgacgcgcag gcgctcctcg gcaagtggac gacggagaag
     1381 atccgcttct acctcgccga cggccgcttc agcgaggacg acacgacgtg cacgttcgac
     1441 ttcaccggca ggcaggtgac gagcgagtgc atggtgcagg gccggctggt gaggatggtg
     1501 tcctcttacg aggtgaaggc gcccggccgc tacgattcga cggtggtcga gaacgcgagc
     1561 tacccgcaga tggtgggcgc gaaggacacg acgcagttcc gggtggagaa gggcaagctg
     1621 gtgcagtcgt cgtatcttcc catctatagc gccgatccga tgcgtcccgt ggagatcgag
     1681 gcgacgctcg ccccgagcgt gctttccggc aggagatccc aatgagcgag tacaaggcgc
     1741 ccgtgcgcga catgcggttc gtcctgaacg agctggccgg cctcgccgac gtggcgcggc
     1801 tgccgggctg cgaggacgtg accgccgagc tggtcgacca gatcctcgag gagagcgcgc
     1861 gcttctcggg cgaggtgctg gcgccgctga accagccggg cgacgaggag ggctcgcact
     1921 gggcggacgg gaaggtgacg acgccgaagg ggttcaagga ggcgtaccag gcgttcatcg
     1981 gcggcggctg gaacgcgctg cagtttccga ccgagttcgg cgggcagggg ctgccgaagc
     2041 tggtggcgac gccggtgatg gagatgtgga agtcggcgaa cctgtcgttc tcgctgtgcc
     2101 cgctgctcac gaccggcgcg gtcgaggcgc tgctgctgcg cgggtcgccg cagctcaaga
     2161 agacgtacct gccgaagatg gtgcagggga cgtggaccgg gacgatgaac ctgaccgagc
     2221 cgcaggcggg caccgatctc gcgctgctgc gcacgaaggc ggtccgcgcc ggcgaccact
     2281 acaagatctc ggggcagaag attttcatca cctacggcga gcacgacctc gccgagaaca
     2341 tcgtgcacct ggtgctggct cgaactccgg acgccccgga gggcgtccgg ggaatctcgc
     2401 tcttcgtcgt gccgaagctc gtgcccaagc ccgacggcac gccgggcgag cgcaacaagg
     2461 tggcgtgcgc gtcgatcgag cacaagctcg gcatccacgc gagcccgacc gcggtgatgg
     2521 tgtacgacgg cgcgaccggc tacctggtgg gcgaggagaa ccgcggcctg gagtacatgt
     2581 tcatcatgat gaacgccgcg cgcttcgcgg tcggcatcga gggaatctcg atctccgagc
     2641 gcgcgtacca gcaggcgctc gcgtacgcaa aagaacgtat acagagccgc gacctgtcgg
     2701 gcgacgccaa agccgtgccg atcatccgcc accccgacgt gcggcgcatg ctgatgcaca
     2761 tgaaggcgat gaccgaggcc atgcgcgcgc tcgcgtacgt cgtcgccgcg ggcctcgacc
     2821 gcgcgcacaa gcatcccgaa aaagaagaac ggatgagagc gcaggccttc gtcgatctca
     2881 tgatccccgt cgtcaagggc tggagcaccg agaccggcgt cgaagtcgcg tcgaccggca
     2941 tccaggtcca cggcggcgtc ggcttcatcg aggagaccgg cgccgcgcag cactaccgcg
     3001 acgcgagaat caccaccatc tacgaaggca ccaccggcat ccaggcgaac gacctcatcg
     3061 gccgcaagat cgcgagggaa ggcggcgcca ccgcgaagga agtcgtcaag atgatgcgcc
     3121 gcgtcgaggc cgagctcgga tccgaggacg aggactccaa cgccatccgc gtcgccttct
     3181 ccgcggccat caccgcgctc gaagaagccg tctcgtggat ctgcatcaac tacaccgccg
     3241 acatcaaagc cgcccacgc
//
LOCUS       DATDMW010000292         5316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_711394, whole genome shotgun sequence.
ACCESSION   DATDMW010000292 DATDMW010000000
VERSION     DATDMW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711394"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VI319_12330"
     CDS             <1..1092
                     /locus_tag="VI319_12330"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY6864676.1"
                     /translation="GEPPRPGARLANPGLARVLERIAGEGEDGFYGGETARALAAFAR
                     AQGGFFDEDDFRAQDASWGEPLRGTYRGVEILETPPPTQGFTVLEMLNLVEPHDVARL
                     EPLGPELVHLLVQAKQIAYNDRDHLLADPEHHPVPTDRLVSKAYARRRAELMDPERAL
                     PWDRIPSYGTLAGDTVFLATADAEGNAVALVHSLYSTFGSGVVAGDTGIVLQNRSAYF
                     SLDPQHPNRLEAGKRPMHTLIASMALRDGRPWHVVGCMGADGQPQIHLQTYVSLIDQG
                     RDIQAALEAPRWLSGRFLLGEPRDLLNMEGRFPDATLRDLERRGHTVNRWPAWNEIAG
                     HAHGITLDPATGALAGGSDPRSDGAAIGY"
     gene            1165..2745
                     /locus_tag="VI319_12335"
     CDS             1165..2745
                     /locus_tag="VI319_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEY6864677.1"
                     /translation="MASLSSADHSTSPPQVQVPRDYNAAHDLIERNIVAGRGGKIAFV
                     DDGGRTTYGQLAERVNRFGGGMRALGFDMEQRVLLALLDTVDFPTAFLGCIKAGIVPI
                     AVNTLLTTRDYEYMLSDSRAKGLIVSEQLWPQFAPLVGKLPFLKTVIVSGADAKGQTA
                     FRDVLAKGSPKLDPAPTTSDDACFWLYSSGSTGAPKGTVHVHSSMILTAELFGRAFVG
                     VREDDVVYSAAKLFFAYGLGNALSFTMAAGATSVLMAERPTPAAVFKRLAEQRATIFY
                     GVPTLYAAMLATPELPKKGALALRLGVSAGESLPAEISTKWAERTGTDLINTIGSTEM
                     LHVFMGCRPGDVRHGTTGVAVPGYDLRLVDEEGRPVKRGELGELQIKGPTAAAYYWNN
                     RAKTLGTFVGEWTRSGDKFSQDAEGYYIYGGRSDDMLKVSGIYVSPAEVEAALIAHEA
                     VLEAAVVGAIDENKLVKPKAFVVLKAGHKPGSAMTQALQQHVKDRLAPYKYPRWIEFI
                     EELPKTATGKIQRFKLRG"
     gene            2778..3158
                     /locus_tag="VI319_12340"
     CDS             2778..3158
                     /locus_tag="VI319_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864678.1"
                     /translation="MAEPRAEDTRSSEIKVDPNALYLEEIFTDRRIGTIRRMTPVTKD
                     GKPDGARPMLYVGETQIMTPAGSLPIAFEIPASSLEQAAEQFGPLAKEAIERTVRELQ
                     ELRRQAASQIVIPQGPAGGKIQLP"
     gene            complement(3180..3605)
                     /locus_tag="VI319_12345"
     CDS             complement(3180..3605)
                     /locus_tag="VI319_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864679.1"
                     /translation="MRKIFAIVFACALAAVAAGCKAPPTQEEKSKVDYGAKPENYEAL
                     IKEYMRTRVNMEGVIFEFRGGPKPLYQQDTVLRPLQYGWGVCVFVHDKRASAFESYPM
                     TFFIRNGVIVAANGGPDDNLIGRRFALADCNELGTPFVR"
     gene            complement(3674..4324)
                     /locus_tag="VI319_12350"
     CDS             complement(3674..4324)
                     /locus_tag="VI319_12350"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY6864680.1"
                     /translation="MAIRIGDEAPDFTAETTEGPIRFHEWIGDKWAILFSHPKDFTPV
                     CTTELGYMAKLKPEWDKRNTKIIGLSVDPVSDHKSWVKDIQETQGTAVNYPMIGDDDL
                     KVAKLYDMIHPNASGGKRTAADNATVRSVFIIGPDKKVKAMLIYPMSAGRNFDEVLRL
                     LDSLQLAAKAAIATPVNWKPGQDVIIPTSVSDDEAKKKYPQGFKTHKPYLRTVSLK"
     gene            complement(4452..4892)
                     /locus_tag="VI319_12355"
     CDS             complement(4452..4892)
                     /locus_tag="VI319_12355"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEY6864681.1"
                     /translation="MLPDIHVNYLAVVAAVVANIVLGFLWYGPVFGSSWRKEMGIAAD
                     ARPAPGAMWRSMSLMVFGAFLMAWVLSYDEAVWRPSSWKAGADQTPAMYGFFAALFTW
                     LGYIVPLLLGGIAWENKSGKLFAINAGYWLVALLAMGMIVAYWR"
     gene            complement(5019..5228)
                     /locus_tag="VI319_12360"
     CDS             complement(5019..5228)
                     /locus_tag="VI319_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864682.1"
                     /translation="MKAWIIGIGAFGMSSATVVAVNPGGVVTNAVNVVQHQVQSVTGT
                     GGSGGAVGGGTTVSAPSITSGCHSH"
BASE COUNT          849 a         1813 c         1815 g          839 t
ORIGIN      
        1 ggggagccgc cgcgaccggg ggcgcggctg gcgaatccgg ggctggcgcg cgtcctcgag
       61 cgcatcgcgg gcgagggtga ggacggcttc tacggcggcg agacggcgcg cgcgctcgcg
      121 gcgttcgcgc gggcgcaggg cgggtttttc gacgaggacg acttccgcgc ccaggacgcg
      181 tcctggggag agccgctgcg gggcacctac cgcggcgtcg agattctcga gacgccgcct
      241 ccgacccagg ggttcaccgt cctcgagatg ctgaacctgg tcgagcccca cgatgtcgct
      301 cgcctggagc cgctgggacc cgagctcgtg cacctgctcg tgcaggcgaa gcagatcgcg
      361 tacaacgacc gcgaccacct gctcgcggat cccgagcatc atcccgtgcc gacggatcgc
      421 ctcgtgtcga aggcgtacgc gcgccggcgc gccgagctga tggaccccga gcgcgcgctg
      481 ccctgggacc gcatcccctc ttacggcacg ctcgcggggg acaccgtttt cctcgcgacg
      541 gcagacgccg aagggaatgc cgtcgccctc gtgcacagcc tgtattcgac gttcggttcg
      601 ggcgtggtcg cgggcgacac gggcatcgtc ctgcagaacc gctcggcata tttctcgctc
      661 gatccgcagc atccgaaccg gctcgaggcc ggaaagcgcc cgatgcacac tctgatcgct
      721 tcgatggccc tgcgcgacgg caggccctgg cacgtcgtcg gctgcatggg cgcggacggc
      781 cagccgcaga tccacctgca gacctacgtc tcgctgatcg accagggccg cgacatccag
      841 gcggcgctcg aggcgccgcg ctggctgtcg gggcggttcc tgctcgggga gccgcgcgac
      901 ctgctcaaca tggagggccg gttccccgat gcgacgctgc gcgatctcga gcgccgtggc
      961 cataccgtga accgctggcc ggcctggaac gagatcgcgg gccacgcgca tggcatcacg
     1021 ctcgatcccg cgaccggtgc gctcgcgggc ggctccgacc cgcgaagcga cggcgcggcc
     1081 atcgggtatt gacctggcgc aactgcacgg cctcgccgcg cgccttacaa tcggcgccat
     1141 aagaccagcc cggaggaggc gcgcttggcg tcactcagtt ccgccgacca ttcgacgagc
     1201 ccgccgcagg tgcaagtccc gcgcgactac aacgccgcgc acgacctgat cgagcgcaac
     1261 atcgtcgccg ggcgcggagg caagatcgcg ttcgtcgacg acggcggccg caccacctac
     1321 ggccagctcg ccgagcgcgt gaaccggttc ggcggcggca tgcgcgcgct cggcttcgac
     1381 atggaacagc gcgtgctgct cgcgctgctc gataccgtcg acttccctac ggcgttcctg
     1441 ggatgcatca aggccggcat cgtgccgatc gccgtgaaca cgctgctcac gacccgggac
     1501 tacgagtaca tgctctccga cagccgcgcc aaggggctga tcgtgtccga gcagctatgg
     1561 cctcagttcg cgccgctcgt cggcaagctg ccgtttctga agaccgtcat cgtgtcgggg
     1621 gcggatgcga aggggcagac cgccttccgg gacgtcctcg cgaaaggctc gcctaagctc
     1681 gatccggcgc cgaccacgag cgacgacgcg tgcttctggc tctactcgtc gggctcgacg
     1741 ggcgcgccca agggcacggt gcacgtgcac tcgagcatga ttctcaccgc cgagctgttc
     1801 ggccgggcgt tcgtcggcgt ccgcgaggac gacgtcgtct actcggcggc gaagctgttc
     1861 ttcgcgtacg ggctggggaa cgcgctgtcg ttcacgatgg cggccggcgc gacctccgtg
     1921 ctcatggccg agcgccccac gcccgccgcg gtgttcaagc ggctcgccga gcagcgggcg
     1981 acgatcttct acggcgtgcc gaccctgtac gcggcgatgc tcgcgactcc ggagctgccg
     2041 aagaagggcg cgctcgcgct gcgcctgggc gtctccgcag gcgaatcgct gcccgccgag
     2101 atcagcacga agtgggccga gcgcacgggc accgatctga tcaacaccat cggctcgacc
     2161 gagatgctgc acgtgttcat ggggtgccgg ccgggcgacg tgcgtcacgg cacgaccggc
     2221 gtcgccgtgc ccggctacga tctgcggctc gtggacgagg aaggccgccc ggtgaaacgc
     2281 ggcgagctgg gcgagctgca gatcaagggc cccaccgctg cagcgtatta ctggaacaac
     2341 cgcgcgaaga ccctcggcac cttcgtcggc gagtggacgc gcagcggcga caagttcagc
     2401 caggatgccg agggctacta tatctacggc ggccgcagcg acgacatgct gaaggtgagc
     2461 ggcatctacg tctcgccggc ggaggtcgag gcggcgctga ttgcgcacga ggccgtgctc
     2521 gaggcggcgg tcgtcggcgc gatcgacgag aacaagctgg tgaagcccaa ggccttcgtc
     2581 gtgctcaagg cgggacacaa gcccggcagc gccatgaccc aggcgctgca gcagcacgtg
     2641 aaggaccgcc tcgcgcctta caagtatccg cgctggatcg agttcatcga ggagctgccc
     2701 aagaccgcga cgggcaagat ccaacgcttc aagctccggg gttgaacccc gagtcccgtt
     2761 tgacggacaa aatccttatg gctgaaccca gagcagaaga cacccggtcg tccgagatca
     2821 aggtcgatcc gaacgcgttg tacctcgagg agatcttcac cgaccgccgc atcggcacga
     2881 tccggcgcat gacgcccgtg acgaaggacg ggaagcccga tggcgcgcgc cccatgctct
     2941 acgtcggcga gacgcagatc atgacgccgg ccgggtcgct gccgattgcg ttcgagatcc
     3001 ccgcgtcttc gctcgagcag gcggccgagc agttcgggcc gctcgccaag gaggcgatcg
     3061 agcgcaccgt gcgcgagctg caggagctgc gccggcaggc ggcctcgcag atcgtcatcc
     3121 cgcaggggcc cgcgggcggg aagatccagc tgccctgatc cgcctccagc ggacggccgt
     3181 cagcggacga acggggtccc gagctcgttg cagtcggcga gcgcgaagcg ccggccgatc
     3241 aggttgtcgt ccggaccgcc gttcgccgca acgatcaccc cgttgcggat gaagaacgtc
     3301 atcgggtacg actcgaacgc gctcgcgcgc ttgtcgtgca cgaacacgca cacgccccag
     3361 ccgtattgca ggggacgcag gacggtgtcc tgctggtaga gcggcttcgg tccgccgcgg
     3421 aactcgaaga tgacgccttc catgttcacg cgggtgcgca tgtattcttt gatcagcgcc
     3481 tcgtagttct ccggcttcgc gccgtagtcc accttcgact tctcctcttg cgtcggcggc
     3541 gccttgcagc cggcagcgac cgcggcgagc gcgcacgcga agacgatcgc aaagatcttg
     3601 cgcatgattt cctctcctgg tttgttgatc gtcgaaacgg gccgggcgcg agcgcgcgcc
     3661 ggccctcgga aggctatttc agcgagacgg tgcgcaggta cggtttgtgc gtcttgaatc
     3721 cctgcgggta cttcttcttc gcctcgtcgt cggacaccga ggtcgggatg atcacgtcct
     3781 ggcccggctt ccagttcacc ggcgtcgcga tggcggcttt cgcagcgagc tgcagtgaat
     3841 cgagcagccg cagcacctcg tcgaaattgc gccccgcgct catcgggtag atcagcatgg
     3901 ccttcacctt cttgtcgggg ccgatgatga acaccgagcg caccgtggcg ttgtcggccg
     3961 ccgtgcgctt tccgccgctc gcgttgggat ggatcatgtc gtagagcttg gcgaccttga
     4021 ggtcgtcgtc gccgatcatc gggtagttca ccgcggtgcc ctgcgtctcc tggatgtcct
     4081 tcacccagct cttgtggtcg ctcaccgggt cgaccgacag gccgatgatc ttggtgttgc
     4141 gcttgtccca ctcgggcttg agcttcgcca tgtagccgag ctcggtggtg cagaccggcg
     4201 tgaagtcctt ggggtgcgag aacaggatcg cccatttgtc gccgatccat tcgtggaagc
     4261 ggatcgggcc ttcggtcgtc tccgccgtga agtcgggggc ctcatcgccg atgcgaattg
     4321 ccatgtggtt ctccaaaaaa attgggcgag ccgggcgggc gcccgtgtga aggccgccgt
     4381 cggcccgcgg gttgccgtgg gaaacgcggt ggatccacaa ttatagcgcc gcggacccgc
     4441 gcccgccgcc gctaccgcca gtaggcgacg atcatcccca tcgcgagcag ggcgaccagc
     4501 cagtagcccg cgttgatcgc gaacagcttc ccggacttgt tctcccaggc gatgccgccg
     4561 agcagcagcg gcacgatgta gccgagccag gtgaagagcg cggcgaagaa gccgtacatc
     4621 gcgggggtct ggtcggcgcc ggccttccag ctcgaaggcc gccacacggc ctcgtcgtac
     4681 gagagcaccc aagccatgag aaaggcgccg aagaccatga gcgacatcga gcgccacatg
     4741 gcgccgggcg ccggtcgggc gtcggcggcg atccccatct ccttgcgcca ggagctgccg
     4801 aagacggggc cgtaccagag aaagccgagc acgatgttgg cgacgaccgc ggcgaccacg
     4861 gccaggtaat tgacgtggat atcgggcagc atgggatacc tccgctgagt gttcgtgttc
     4921 ggaggaggcg cgattcggcc gcgcgccccg gcccggcggg catgcagccc ccggcgccgc
     4981 ccggaatcat aatccggcgc cgaggcgggc gctgcgggct agtgcgagtg gcacccgctg
     5041 gtgatcgagg gcgcgctgac ggtcgtgccg ccgcccaccg cgcctccgct gcctccggtg
     5101 ccggtgacgc tctgcacctg gtgctgcacg acgttgaccg cattcgtgac cacgccgccc
     5161 gggttcaccg ccacaaccgt cgccgaagac atgccgaacg ccccgatacc gatgatccac
     5221 gctttcatga caattctccg cgtcgtttca gaagcggcgc ggagcgcgcg aacctcgcgt
     5281 accgcgccga ggcgccattc tgtcgagcgg cggtga
//
LOCUS       DATDMW010000293         4852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2080310, whole genome shotgun sequence.
ACCESSION   DATDMW010000293 DATDMW010000000
VERSION     DATDMW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080310"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VI319_12365"
     CDS             <1..910
                     /locus_tag="VI319_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6864683.1"
                     /translation="LAQSWPARPVRLIVSQAAGGTPDIVARLLAQRVGDALGQPLIVE
                     NRPGSANMVGAQAAARATPDGYTLFFATAAALVTNPYTFKSLPYDPVKDFAPIGMAGK
                     AMFLILAHPSVPARDIKELVALDKADPGKLAFATDGPRNFSGMVAAWLNKLMGTQILA
                     VPYANMPQGVQDTLAGRTQLVVLAVPAAAAFIRRGDLRPIAETFARRIPGYENVPPIA
                     ETFPGFEFIGWFAFVAPAGTPKDVVGAMNREIDKVLQDPEVRQRLATLGFYTEGGDTP
                     QGTGRFVRSELAKWGRIVKEIGIQPE"
     gene            915..1892
                     /locus_tag="VI319_12370"
     CDS             915..1892
                     /locus_tag="VI319_12370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864684.1"
                     /translation="MSRPSLVMLVVAATLATGVCAQSYPAKPVHLIVPYPAGGVVDGL
                     MRRIGQPVSETLGQPVVVDNRPGANTIIALEACARAAPDGYTICSSSSDGMSFNPWLY
                     PKLPYDAERDFAPITQLVWVNGVIVAGAKVPYGTVAEMVAYARANPGKVNFASFGVGS
                     TPHFFLEWFRKQANVDIVHVPYKGSAQIIPALLSGEADATFIAMGIVLPMIASGRMKA
                     LAVTGAERSPYLPEVSTLAEQKLDPKIQNWFGVFAPAHTPTAVVERLNAEFLRALGNP
                     KFREFLRAQAFDAVGNNPREFAQFLRADRANARAVIARTGIRLEDAGTR"
     gene            1909..2427
                     /locus_tag="VI319_12375"
     CDS             1909..2427
                     /locus_tag="VI319_12375"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6864685.1"
                     /translation="MRRVVTGHDTLGRSVVLEDRDSPHRSVLDEAGGLVLTELWETRS
                     APADNRGDADAARRERRIEPVNGGTVFRVIEYPPDRERLRSIAPEAHFASMGARAADA
                     ATRRHPGMHRTDTIDYCIVLSGEIWAVLDEGEVLLRAGDCLVQRGTNHAWSNRRDAPC
                     TIAFVLVGAERT"
     gene            2424..4121
                     /locus_tag="VI319_12380"
     CDS             2424..4121
                     /locus_tag="VI319_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY6864686.1"
                     /translation="MIVDRDVAVPMRDGASLRANVYRPGDENAPSGRCPVLMTLGPYG
                     KDVHLSQFMPEAWEALKRRHPEILRASSCKYLVFETPDPEVWTREGYVIVKVDSRGAG
                     KSPGRLDVNSPAEFRDFYDAIEWAGVQPWSSGKVGLLGISYYAAGQWMIAAERPPHLA
                     AILPWQGTCEFYRDRTRQGGILGNGFLNRWWNRSVLRNQHGNPDTPLVDIDTGERNTG
                     PARLSPAQLAANRVDYIAELRARPLDGPWYDERSGHLERIELPALVVANWGGLGLHLR
                     GTLLGWRGIASREKWLKVHAGSYFLTFLLPDSVALQRRFFDRYLKGLDNGWEREPRVE
                     VSVRAVDDTVKRVVADRDWPIPGTRWTRLYLDAASVSLTPSPPRAAASAGYDATGEGV
                     TFSTAPLERDLELAGPIVAKLWVSSSTDDMDVFATLRAFGPDGREATFFHATEPRAPV
                     SQGWLRVSHRKTDPRRSTDWQPRHTHDAIEPLVPGQVYEIDVEIWPASLALPAGWRLA
                     LTLQGVDFQRPGEPGAQRGSGWFLHDDPEDRPPARFHGRHTVHTGPERAAHLLLPVL"
     gene            4124..4810
                     /locus_tag="VI319_12385"
     CDS             4124..4810
                     /locus_tag="VI319_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY6864687.1"
                     /translation="MERASSAVSQARRKAASGPGLRERNKLDKRQRIRAAAADLFARL
                     GYGAATMRQIAKRAHVGLGTLFNYAEDKRDLVFLIFNDELAALTDAALAAPAADEPLI
                     DQVMAVFACHYRRLGENHALSRILLQELTFYSAGKQAAAFQDIRKRLIDGIEVLVRRA
                     QGDGRILAREDPAFVALHLFFVYSAAIRWWITAPHPDVREGLAELRRLLELQFAGLSP
                     APGPARRLNR"
BASE COUNT          696 a         1782 c         1676 g          698 t
ORIGIN      
        1 gctcgcgcag tcctggccgg cgcgtccggt gcggctgatc gtgtcgcagg cggccggcgg
       61 cacgcccgac atcgtggcgc ggctgctcgc ccagcgcgtg ggcgatgcgc tcgggcagcc
      121 gctcatcgtg gaaaaccgcc cgggctcggc caacatggtg ggcgcgcaag ccgcggcgcg
      181 cgcgacgccg gacggctaca cgctcttctt cgcgaccgcc gcggcgctcg tcaccaatcc
      241 ctacacgttc aagtcgctgc cctacgatcc ggtgaaggac ttcgccccga tcggcatggc
      301 cgggaaggcg atgttcctga tcctcgcgca tccgagcgtc ccggcaaggg acatcaagga
      361 gctggtggcc ctcgacaagg ccgatcccgg aaagcttgcg ttcgccaccg acggcccgcg
      421 caacttctcc ggcatggtcg cggcgtggct gaacaagctg atgggcaccc agatcctcgc
      481 cgtgccctac gccaacatgc cgcagggcgt gcaggacacg ctcgccggcc gcacgcagct
      541 cgtcgtgctc gcggtgccgg cggcggcggc gttcatcagg cgcggcgacc tgcggcccat
      601 cgccgaaacc ttcgcgcggc gcatccccgg atacgaaaac gtcccgccga tcgcggagac
      661 ctttccgggc ttcgagttca tcggctggtt cgcgttcgtc gcgccggccg gaacaccgaa
      721 ggacgtggtg ggcgcgatga accgcgagat cgacaaggtg ctgcaggatc cggaagtccg
      781 ccagcgcctc gcgacgctcg ggttctacac cgagggcggc gacacgcccc agggcaccgg
      841 ccgcttcgtc cggagcgagc tcgcgaaatg gggccgcatc gtcaaggaaa tcgggatcca
      901 gccggagtga cgccatgagc cgaccctccc tcgtcatgct cgtcgtcgcc gccacgctcg
      961 cgacgggcgt gtgcgcccag tcctatccgg cgaagccggt gcacctcatc gtgccgtatc
     1021 cggccggcgg cgtcgtcgac gggctcatgc gccgcatcgg gcagccggtc tcggagacgc
     1081 tcgggcagcc cgtggtcgtc gacaaccgtc ccggcgcgaa caccatcatt gcgctcgagg
     1141 cctgcgccag agccgcgccc gacggctaca cgatctgcag ctcctcgagc gacggcatgt
     1201 cgttcaaccc ctggctatat ccgaagctgc cgtacgacgc cgagcgcgat ttcgccccga
     1261 tcacgcagct cgtctgggtg aacggcgtca tcgtcgccgg cgccaaggtc ccgtacggca
     1321 ccgtcgcgga gatggtcgcg tacgcgcgcg caaatccggg caaggtgaac ttcgcctcct
     1381 tcggcgtcgg cagcacgccg catttcttcc tcgagtggtt ccgcaagcag gccaatgtcg
     1441 acatcgtgca cgtgccctac aagggctcgg cgcagatcat cccggcgctg ctctcgggcg
     1501 aggccgacgc gaccttcatc gccatgggca tcgtgctgcc gatgatcgcc tcgggccgga
     1561 tgaaggcgct ggcggtgacc ggcgccgagc gctcgcctta cctgcccgag gtgtcgacgc
     1621 tcgcggagca gaagctcgac ccgaagatcc agaactggtt cggcgtgttc gcccccgcgc
     1681 acacgccgac cgcggtcgtc gagcgcctga acgcggagtt cctgcgcgcc ctgggcaatc
     1741 cgaagttccg ggagttcctg cgcgcgcagg cgttcgacgc ggtggggaac aatccgcgcg
     1801 agttcgccca gttcctgcgc gccgaccgcg ccaacgcgcg cgcggtgatc gcgcgcaccg
     1861 gcatccggct ggaggacgcc gggacgcgtt gagtcccggc gctgcgacat gcgcagggtc
     1921 gtgaccggcc atgacaccct cggccgctcg gtcgtgctcg aggaccgcga ttccccgcac
     1981 cgctcggtgc tcgatgaggc cggcggcctg gtgctgaccg agctgtggga gacgcgctcg
     2041 gcgcccgcgg acaaccgcgg cgatgccgac gcggcgcgcc gcgagcgccg catcgagccc
     2101 gtgaacggcg gcaccgtgtt ccgcgtcatc gaatatccgc ccgatcgcga gcggctgaga
     2161 tcgatcgccc ccgaggcgca cttcgcgagc atgggcgcgc gcgcggccga cgccgcgacg
     2221 cggcgccatc ccggcatgca tcgcaccgat acgatcgact attgcatcgt gctctcgggc
     2281 gagatctggg cggtgctcga cgagggcgag gtgctgctgc gggccggcga ctgcctcgtg
     2341 cagcgcggca ccaaccacgc ctggagcaac cgcagagacg cgccgtgcac gatcgcgttc
     2401 gtgctcgtcg gcgcggagcg gacgtgatcg tcgaccgcga cgtcgcggtg ccgatgcgcg
     2461 acggcgcgag cctgcgcgcg aacgtgtacc gccccggcga cgaaaatgcg ccatcggggc
     2521 gctgcccggt cctgatgacg ctgggaccct acgggaagga cgtgcatctc tcgcagttca
     2581 tgcccgaggc ctgggaagcg ctgaagcgcc gccaccccga gatcctgcgc gcgtcctcgt
     2641 gcaaatacct ggtgttcgag acgcccgatc ccgaggtgtg gacgcgagag ggctatgtca
     2701 tcgtgaaggt cgactcgcgc ggcgccggca agtcgcccgg ccgcctcgac gtgaactccc
     2761 ccgccgagtt ccgcgacttc tacgacgcga tcgagtgggc gggcgtgcag ccgtggagca
     2821 gcggcaaggt cgggctgctc ggcatctcgt actacgcggc cgggcagtgg atgatcgccg
     2881 ccgagcgccc gccgcacctg gccgcgatcc ttccctggca gggcacctgc gagttctacc
     2941 gcgaccgcac gcgccagggc ggaattctcg gcaacggctt cctcaaccgc tggtggaacc
     3001 gcagcgtgct gcgcaaccag cacggcaacc cggacacgcc tctcgtcgac atcgacaccg
     3061 gcgagcgcaa caccgggccg gcgagactct cgccggccca gctcgcggcg aaccgcgtcg
     3121 actacatcgc cgagctgcgc gcgcgtccgc tggacggtcc gtggtacgac gagcgcagcg
     3181 gccacctcga gcgcatcgag ctgccggcgc tcgtggtcgc gaactggggc ggcctcggcc
     3241 tgcatctgcg cggcaccctg ctgggctggc gcggcatcgc ctcgcgcgag aagtggctga
     3301 aggtccatgc cggctcgtac ttcctcacct tcctgctgcc ggacagcgtc gcgctgcagc
     3361 ggcgcttctt cgatcgctac ctgaagggcc tcgacaacgg ctgggagcgc gagccgcgcg
     3421 tcgaggtctc ggtgcgcgcg gtcgacgaca cggtgaagcg cgtcgtggcc gatcgcgact
     3481 ggccgatccc cggcacgaga tggacacgac tctatctcga cgccgcgagc gtttcgctca
     3541 cgccgtcgcc gccgcgcgcc gcggcgagcg ccggctacga cgcgacgggc gaaggcgtca
     3601 ccttctccac cgcgccgctc gagcgcgacc tggagctcgc gggtcccatc gtcgcgaagc
     3661 tctgggtctc gtcgagcacc gacgacatgg acgtgttcgc gacgctgcgc gcgttcggcc
     3721 ccgacggccg cgaggcgact ttttttcacg cgaccgagcc gagggcgccg gtgtctcagg
     3781 gctggctccg cgtctcgcat cgcaagaccg acccgcggcg ctcgacggac tggcagccgc
     3841 ggcacacgca cgacgcgatc gagccgctcg tccccggcca ggtctacgaa atcgacgtgg
     3901 agatctggcc cgcgagcctc gcgcttccgg cgggctggcg actcgcgctc acattgcagg
     3961 gcgtcgactt ccagcgcccg ggcgagcccg gcgcgcagcg tggctcgggc tggttcctgc
     4021 acgacgaccc cgaggaccgg ccgcccgcgc gcttccacgg ccggcacacc gtgcataccg
     4081 ggcccgagcg ggcggcgcac ctcctgctcc cggtgctgtg accatggagc gcgcttcctc
     4141 cgccgtctcc caggcgcgcc gcaaggcggc ctcgggcccg ggcctgcgcg agcgcaacaa
     4201 gctcgacaag cgccagcgca tccgggccgc ggccgcggac ctgttcgcgc ggctgggcta
     4261 cggcgcggcc acgatgcggc agatcgcgaa gcgcgcgcac gtggggctcg ggacgctgtt
     4321 caactacgcc gaggacaagc gcgacctggt gttcctcatc ttcaacgacg agcttgccgc
     4381 gctcaccgac gccgcgctcg ccgcccccgc cgccgacgag ccgctcatcg accaggtgat
     4441 ggcggtcttc gcctgccact accgccggct cggcgagaac cacgcgctct cgcgcatcct
     4501 gctgcaggag ctgacgttct actcggcggg caagcaggcg gcggcgttcc aggacatccg
     4561 caagcggctg atcgacggca tcgaggtgct ggtgcgccgg gcgcagggcg acggccgcat
     4621 cctcgcgcgc gaggatccgg cgttcgtcgc gctgcacctg ttcttcgtct actccgcggc
     4681 gatccgctgg tggatcaccg cgccgcatcc cgacgtccgc gaagggctcg cggagctgcg
     4741 gcggctgctc gagctgcagt tcgcggggct gtcgcccgcg cccggtccgg cgcggcgact
     4801 caaccgctga cgatctccac gtcgggcgtc cacaggcgcg tccagcgcgc tt
//
LOCUS       DATDMW010000294         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_216220, whole genome shotgun sequence.
ACCESSION   DATDMW010000294 DATDMW010000000
VERSION     DATDMW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216220"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VI319_12390"
     CDS             <1..328
                     /locus_tag="VI319_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864688.1"
                     /translation="DRAIRDLLSLHLSNRGYDVAVAEDAVVGGRLALSARPQLIIVDV
                     NMPYLDGPDFVAALKSDPETKDIPVVFLTSDDDLVYRAGHLGAFACLTKPIMADRLLD
                     VVARAL"
     gene            complement(344..1693)
                     /locus_tag="VI319_12395"
     CDS             complement(344..1693)
                     /locus_tag="VI319_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY6864689.1"
                     /translation="MDAMSQRLVPPRVVPPEAPLGRLAFIAAFVRNPLEALPRVVYEQ
                     DVVPYRDRVWITAPALVRKVLLDDRERYRKLSSIRLLGPLLGRGILTSEGAEWKWQRQ
                     AAAPAFRQQDLLPLVPAFVAATERLVARWRARPGEQAIDRDMTRVTFEVISGTLLPSA
                     DASLGAVFERSMGGFQKAAGWSLFFASTGLPAWLPRPGMLTSARSVRALRGATHSVLQ
                     ERKSAPGEGGELLQRLIAARDPETGRSMDDTRLVDNLLTFYLAGHETTARALAWTLYL
                     LARSPQWSSLLEEEVDAVTGGAPVAPEHIERLTLVQQVLKESMRLYPPAPMLARQAVA
                     EAEIGGERVRPGMSVVMPIYAIHRHRSRWSEPDVFDPSRFSGDNEASIERYQYLPFGA
                     GPRICIGMAFAMIEATAMLATMLQRARFSALEGRDPYPVARVTLLPRGGITLNVTTK"
     gene            1839..2441
                     /locus_tag="VI319_12400"
     CDS             1839..2441
                     /locus_tag="VI319_12400"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HEY6864690.1"
                     /translation="MNTTNERRLAAVLAAAALLGAAGAQAQPRGERHEHVRYATPHWV
                     FDDRYHHNHYYPAVGYAVDVLPPGNIAVRFRGGDFWFHSGVWYRHAGPRFVVVRPPVG
                     VLVPVLPPAYSVVYYAGVPYYYANDVYYVQQPTGYEVVDPPPDQAPATASAPGGGATP
                     APASGTWYFCQSANGYYPYVQQCPEGWKSVPATPPPGAPR"
BASE COUNT          379 a          918 c          917 g          359 t
ORIGIN      
        1 cgaccgggcg atccgcgacc tgctgagcct gcatctctcg aaccgcggct acgacgtcgc
       61 ggtcgccgag gacgccgtgg tcggcggccg cctcgcgctg agcgccaggc cgcagctcat
      121 catcgtcgac gtcaacatgc cgtacctgga cgggccggac ttcgtcgccg cgctgaagag
      181 cgatccggag acgaaggaca tcccggtcgt gttcctgacg agcgacgacg acctcgtcta
      241 ccgcgccggc cacctcggcg cctttgcctg cctgaccaag ccgatcatgg ccgaccggct
      301 gctcgacgtc gtcgcgcgcg cgctctagcg tcccgcaggc cggctatttc gtcgtgacgt
      361 tcagcgtgat gccgccgcgc ggcagcagcg tgacgcgcgc cacgggatag ggatcgcgtc
      421 cttccagcgc cgagaagcgc gcgcgctgga gcatcgtggc gagcatcgcc gtcgcctcga
      481 tcatcgcgaa cgccatgccg atgcagatgc gcggccctgc gccgaagggc aggtactggt
      541 agcgctcgat ggaagcctcg ttgtcgcccg agaagcgcga cgggtcgaag acgtccggct
      601 ccgaccagcg gctgcggtgg cggtggatgg cgtagatcgg catcaccacc gacatgccgg
      661 ggcggacgcg ctcgccgccg atctcggcct ccgcgaccgc ctgccgcgcg agcatcggcg
      721 ccggcggata gaggcgcatc gactccttga gcacctgctg cacgagcgtg aggcgctcga
      781 tgtgctcggg cgcgaccggc gcgccgccgg tcaccgcgtc gacctcctct tcgaggagcg
      841 acgaccattg cggcgagcgg gcgagcaggt acagcgtcca cgcgagcgcg cgcgccgtgg
      901 tctcgtggcc ggcgagatag aaggtgagca ggttgtcgac gaggcgcgtg tcgtccatgg
      961 agcggccggt ctccggatcg cgcgcggcga tcaggcgctg cagcagctcg ccgccttcgc
     1021 ctggcgccga ttttctctcc tgcagcacgc tgtgcgtcgc gccgcgcagc gcccgcaccg
     1081 atcgcgcgct cgtgagcatc cccgggcgcg gcagccacgc gggcagcccg gtcgaggcga
     1141 agaaaaggct ccagccggcc gccttctgga agccgcccat cgagcgctcg aacacggcgc
     1201 cgaggctcgc gtccgccgag ggcaggagcg tgcccgagat cacctcgaag gtgacgcgcg
     1261 tcatgtcgcg gtcgatcgcc tgctcgccgg gcctcgcgcg ccagcgcgcg acgaggcgct
     1321 cggtcgcggc gacgaacgcc ggcacgagcg gcagcaggtc ctgctgccgg aacgcgggcg
     1381 ccgcggcctg gcgctgccat ttccactcgg cgccttcgct cgtcaggatg ccgcgcccga
     1441 ggagcggccc gagcagccgg atgctggaga gcttgcggta gcgctcgcga tcgtcgagca
     1501 gcaccttgcg cacgagcgcg ggcgcggtga tccacacgcg gtcgcggtac ggcacgacgt
     1561 cctgctcgta gaccacgcgc ggcagcgcct cgagcgggtt gcgcacgaac gcggcgatga
     1621 acgccagccg gccgagcggc gcttcggggg gtacgactcg cggtggaacg agccgctggc
     1681 tcatcgcgtc catgtcgacg ggcgtgagct taaggtcctt aaggcaggaa ctccacaggc
     1741 gtctgacgcg aacgaaagcc tggcaacacc aatggataca aatcgcgaca cagccgggcg
     1801 gcgcgcttcc ctaagatcgc gccatgcaag gaggcttcat gaatacgacg aacgagcgaa
     1861 ggctggccgc ggtattggcc gccgcggcgc tgctcggcgc cgccggcgcg caagcgcagc
     1921 cgcgcgggga gcgccacgag cacgtccgct acgcgacgcc gcactgggtg ttcgacgacc
     1981 gctaccacca caaccactat tacccggcgg tcggctatgc ggtcgacgtg ctgccgccgg
     2041 gaaacatcgc cgtgcgcttc cgcggcggcg acttctggtt ccactcgggc gtctggtacc
     2101 ggcacgccgg gccgcgcttc gtggtcgtgc gtccgccggt cggcgtcctc gtgccggtcc
     2161 tgccgccggc gtattcggtc gtctactacg ccggcgtgcc gtactactac gcgaacgacg
     2221 tgtattacgt gcagcagccg accggctacg aagtggtgga cccgccgccc gaccaggcgc
     2281 cggccacggc gagcgcgccc ggcggcggcg cgacgccggc tccggcctcc ggcacgtggt
     2341 acttctgcca gtcggcgaac ggctattacc cgtacgtgca gcaatgcccg gaaggctgga
     2401 agagcgttcc cgccacgccg ccgcccggcg cgccgcgatg aggcgcccgg ctaggcgagg
     2461 gcctcgcgga ggcaggactc gagcagctcg agcgcgcgca gcgtaaaggc gcgcatgttc
     2521 tcttcgcggg ctgcgcgtcc ggtctctagc gtgacgacct tctcgacggg gcc
//
LOCUS       DATDMW010000295         2410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2331869, whole genome shotgun sequence.
ACCESSION   DATDMW010000295 DATDMW010000000
VERSION     DATDMW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331869"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..554)
                     /locus_tag="VI319_12405"
     CDS             complement(84..554)
                     /locus_tag="VI319_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6864691.1"
                     /translation="MSESRPPFPPFSRDTAAQKIRGAEDAWNTRDPERVSLAYTPDSV
                     WRNRSEFFAGRPAIVEFLKRKWAKEIDYRLIKELWAFHENRIAVRFAYEWRDDAGSWF
                     RSYGNENWEFAENGLMRRRIASINDLPIREAERKYKWPLGRRPDDHPGLSDLGL"
     gene            complement(551..>2410)
                     /locus_tag="VI319_12410"
     CDS             complement(551..>2410)
                     /locus_tag="VI319_12410"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6864692.1"
                     /translation="GSATVTGYWDVARTFGAQVRRVLMDNAARAWSVPASELSTEPGA
                     VVHAKSGRRMSYGEIAAIAVVPSQAPAIRPEELKKPEQYRLIGKDVMRVELPTKVDGS
                     ARYSIDVQAPGMLYAAVLRGPVEGTSPVEIDERAARAVPGVGRIVRLPFGVGVIAETP
                     WAAFQAKDALNVTWDRNARAFGHDSDKAVGVYSAAARDLSRPGKPWDAAGDAPAAMKS
                     AAKVYEAEYACDYTYHAQMEPLNSVASVSPAGDSCEIWCGTQSQTMAVAAVAKALNIA
                     VEKVKLNLMLLGGGFGRRGHRDEEFVVDSVLLSREVKRPVKVLWTREDDVRNGRFRPL
                     SVHYLRAGLDAGGRIVAWQHRVACDEITAFQDPVRYKGGGEKDFLAMAGSELRTYDIP
                     NRLSEQLPQATGIRTSSLRGIGFGPNKFATEAFLDEIAVQHGIDPVELRLQLLKNTPR
                     GQAVVREVVAMSDYRRARPGRGLGFAFTDYSGTMVAAVAEVSVDKASGAVKVHDFWLA
                     LDPGIAVQPDNVVAQTESSIVYGLGLALTERITFRDGVVQQRNIRDYGVPRMADIPEI
                     HIKLVSTPNRPTGAGQMATPIVAPAIASAVHAASGARVRHIPFLPERVKAVVA"
BASE COUNT          330 a          889 c          809 g          382 t
ORIGIN      
        1 caacggatca ttgtaatggt ctcccgggac ccgggggcgc tgttcccgag tatttcgatg
       61 acagcgcccg tagtgctttc ggtctacaga ccgaggtcgg agagccccgg atgatcgtcg
      121 ggccgccggc cgagcggcca cttgtacttg cgctcggcct ccctgatcgg gaggtcgttg
      181 atgctcgcga tccgccgccg catcaggccg ttctcggcga attcccagtt ctcgttgccg
      241 taggagcgga accagctgcc ggcgtcgtcg cgccactcgt acgcgaagcg cacggcgatg
      301 cggttctcgt ggaaggccca cagctccttg atcaggcggt agtcgatctc cttcgcccac
      361 ttgcgcttca ggaactcgac gatcgccggg cgccccgcga agaactccga gcggttcctc
      421 cacacgctgt cgggcgtgta cgcgagcgac acgcgctcgg ggtcgcgcgt gttccacgcg
      481 tcctcggcgc cgcggatctt ctgcgccgcc gtgtcgcggc tgaacggcgg gaacggcgga
      541 cggctctcgc tcatgcgacg accgccttca cgcgctcggg caggaacggg atgtgccgca
      601 cgcgcgcacc gctcgccgcg tggacggcgc tcgcgatcgc gggcgcgacg atcggcgtcg
      661 ccatctggcc cgcgcccgtc ggccggttcg gcgtcgacac gagcttgatg tggatctcgg
      721 ggatgtcggc catccgcggc acgccgtagt cgcggatgtt gcgctgctgc accacgccgt
      781 ccctgaacgt gatgcgctcg gtgagcgcga gcccgaggcc gtagacgatc gagctctcgg
      841 tctgcgccac gacgttgtcg ggctgcaccg cgatgcccgg gtcgagcgcg agccagaagt
      901 cgtgcacctt caccgcgccg ctcgccttgt cgaccgagac ctcggcgacc gcggcgacca
      961 tcgtccccga gtagtcggtg aacgcgaacc cgaggccgcg gccgggacgc gcgcgccggt
     1021 agtcggacat cgccaccacc tcgcgcacca cggcctggcc gcgcggcgtg ttcttcagca
     1081 gctgcaggcg cagctcgacc ggatcgatgc cgtgctgcac ggcgatctcg tcgaggaagg
     1141 cctcggtcgc gaacttgttc ggcccgaagc cgatgccgcg caagctcgac gtccgaatgc
     1201 ccgtcgcctg cggcagctgc tcggagagcc ggttcgggat gtcgtaggtg cgcagctccg
     1261 agcccgccat cgcgaggaag tccttctcgc cgccgccctt gtagcgcacc gggtcctgga
     1321 acgcggtgat ctcgtcgcac gcgacgcggt gctgccacgc cacgatgcgg ccgccggcgt
     1381 cgagtcccgc gcgcaggtag tgcaccgaga gcggacggaa gcggccgttc ctcacgtcgt
     1441 cctcgcgcgt ccacagcacc ttcaccgggc gcttcacttc cctggacagg agaaccgagt
     1501 cgaccacgaa ctcctcgtcg cggtgcccgc ggcggccgaa gccgccgccg aggagcatga
     1561 ggttcagctt caccttttcg accgcgatat tcagcgcctt cgcgaccgcc gcgaccgcca
     1621 tggtctgcga ctgcgtgccg caccagatct cgcagctatc gcccgcgggc gataccgagg
     1681 ccaccgagtt gagcggctcc atctgcgcgt gatacgtgta gtcgcacgcg tactccgcct
     1741 cgtagacctt cgcggcgctc ttcatcgccg ccggcgcgtc gcccgcggcg tcccacggct
     1801 tgccgggccg cgacagatcg cgcgccgccg ccgagtagac gccgaccgcc ttgtcgctgt
     1861 cgtgcccgaa agcgcgggca ttgcggtccc aggtgacgtt cagcgcgtcc ttcgcctgga
     1921 acgccgccca cggcgtctcg gcgatcacgc ccacgccgaa cggcaggcgc acgatgcgcc
     1981 cgacgcccgg caccgcgcgc gccgcgcgct cgtcgatctc caccggcgag gtgccctcga
     2041 ccggcccgcg cagcaccgcg gcgtagagca tgcccggcgc ctgcacgtcg atcgaatacc
     2101 gggcgctgcc gtcgaccttg gtcggcagct cgacccgcat gacgtctttt ccgatcaggc
     2161 ggtactgctc gggcttcttg agctcctcgg gcctgatcgc cggcgcctgc gaagggacga
     2221 ccgcgatcgc ggcgatctcg ccgtagctca tgcggcgacc ggatttcgcg tgcaccaccg
     2281 cgcccggctc ggtcgagagc tccgacgcgg gcacgctcca ggcgcgcgcc gcgttgtcca
     2341 tcagcacgcg gcgcacctgc gcgccgaagg tgcgcgcgac gtcccagtat ccggtgacgg
     2401 tcgcggaacc
//
LOCUS       DATDMW010000296         4216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_959752, whole genome shotgun sequence.
ACCESSION   DATDMW010000296 DATDMW010000000
VERSION     DATDMW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959752"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VI319_12415"
     CDS             <1..336
                     /locus_tag="VI319_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864693.1"
                     /translation="ATLAAGALFRLYDQRVNESSVVAEIPWTDAQQTHFSAVVTRYSD
                     GVSLMNVCAVTSGGQTCSPLVSVAGSTSPDASGGISWGASGGQTSGPYRAPSAPANLP
                     NAAPSSLQR"
     gene            957..1727
                     /locus_tag="VI319_12420"
     CDS             957..1727
                     /locus_tag="VI319_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864694.1"
                     /translation="MSVPEDPSELPSSRDARHVRLTWSNQVDVALKYPMPLVPRVTHT
                     EGAVNEVRFFWVNGSDVTDSYSIERLHYALNDKALMRSGTAAEPSTVRPSPFAANAAR
                     PPAPVADNRIWVEWLNVPPVVARACKGAELCASDKTLKVIATAGPAVEPMQYRVCAAN
                     RAGRACSEPVSPNFSVQRIVPTPPAPSVVKVQPPPPSNAHLACIIGKDPSDPTRQCAV
                     SEKALGATCKCPRIYGEGRVGWSTYIPQEKRRALGFGR"
     gene            1857..2063
                     /locus_tag="VI319_12425"
     CDS             1857..2063
                     /locus_tag="VI319_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864695.1"
                     /translation="MPIFEVTYKAVCPGCGHHWKTTARREANDKIDASESAPAMQCES
                     CKAFTVPYRVIVGAAGGATATIAR"
     gene            complement(2088..2163)
                     /locus_tag="VI319_12430"
     tRNA            complement(2088..2163)
                     /locus_tag="VI319_12430"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2127..2129),aa:Glu,seq:ttc)
     gene            complement(2190..2265)
                     /locus_tag="VI319_12435"
     tRNA            complement(2190..2265)
                     /locus_tag="VI319_12435"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2230..2232),aa:Ala,seq:ggc)
     gene            2618..2872
                     /locus_tag="VI319_12440"
     CDS             2618..2872
                     /locus_tag="VI319_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864696.1"
                     /translation="MKSILDRSFRYTPSVETDLRKTFARIRRKQRDKVRAIANADAEV
                     KTKVLLIDHLKKASNAAFAKPVEQRGIALDDVGAVPNRGK"
     gene            2920..>4216
                     /locus_tag="VI319_12445"
     CDS             2920..>4216
                     /locus_tag="VI319_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEY6864697.1"
                     /translation="MAAAFLLWALACRTALAFGFDDVAQRAKALADAPFKKGAGLPKE
                     LQGLDYDHYRDIRFRPDRFLWRGTSSPFELAFFHLGLYYDQAVRINEVSASGVREIPF
                     DPDDFDYGSNKLDVAKLKEEKGLGFAGFRAHYALNTPKYKDEVAVFLGASYFRALGKG
                     QIYGLSARGLAIDTALPSGEEFPRFTEFWIERPAAGAKELVVYALLDSRRAAGAYRFV
                     VQPGVSTAVEVKARLFLRESVGKLGIAPLTSMFFFGENQRPPYDDYRPEVHDSDGLQV
                     RTGAGEWIWRPLVNPKRLLVTSFATTDPGGFGLMQRDRAFAHYEDTEVRHELRPSVWV
                     EPKGKWGAGRVELVEIPAPDETNDNIVAYWVPDAAPKPKEALDLEYRLLWQKDSETRP
                     PLARTVQSRRGRGYMRNPDNSIGFVVDFQGAPLQTLAADA"
BASE COUNT          754 a         1398 c         1392 g          672 t
ORIGIN      
        1 gccacgctcg ccgcaggcgc attgttcagg ctgtacgacc agcgcgtgaa cgagtcgtcc
       61 gtcgtcgcgg agattccgtg gaccgacgcg cagcagacgc acttcagcgc ggtggtgacg
      121 cgctattccg acggcgtctc gctcatgaac gtgtgcgccg tgacgagcgg cggacagacg
      181 tgctcgcccc tcgtttcggt cgcgggcagc acttcgcccg acgcatcggg cggcatcagc
      241 tggggagctt cgggcggaca gacgtcggga ccttatcgcg cgccgagcgc gccggcgaac
      301 ctgccgaacg ccgcgccgag cagcctgcag cgctgataca gaagggagga gacatgctcc
      361 ggaacaatcg cttcgtgcgg gcgatggccc tcgcactcct gggtcacacc tcgctcttcg
      421 ccgcgggcga agttcgtgcc gtaccgccga ggccgccggt tctgaccgtc acctccggcg
      481 attatggatt tcgcatcagc gatgattctg cctcgcgccg cctcgtgagg tctttgtgcg
      541 gagattgcta cgtaaaggca actctggacg gacgggacat aagcggacat caaatcgatg
      601 ggccaacaag aaaggatccc gaagccgatc tcaacgcggt gggtcttctg ctcgagatcc
      661 cgccccggca cttggatcct ggccggaaag tccgttattc cgcgcaatac tgctctcgag
      721 caacgaatga gtgttcaccc gtgtctgcgg ttcgaatcgc catatccgag aagagaatcc
      781 tgagcgccgc cgatgcctct cgctgggagg gaggccacta tgcgtggacg atccgggtcc
      841 ggtggagctc ggttgcgccg tgtgtacgcg agaccggcta cgtgaaatgc gaaatcgcct
      901 tcagcgctcc ggacggatcg acggacaaag actcgagggg cgaaacgaat ctggacgtga
      961 gcgtgcccga ggacccctcg gagcttccca gctcgcggga tgcgcgccac gtgaggctga
     1021 cctggtccaa tcaggtcgat gtcgccctga agtacccgat gccgctcgtt ccgcgcgtca
     1081 cgcacacgga aggggcggtg aacgaagtcc ggttcttctg ggtcaacggg tccgacgtga
     1141 ccgacagcta ctccatcgag cgcctgcatt acgcgctgaa cgacaaggcg ctgatgaggt
     1201 cgggcacggc cgccgagccc tctaccgtcc gcccgtctcc cttcgcggcg aacgccgcgc
     1261 ggcctccggc acccgtcgcc gacaaccgca tctgggtgga atggctgaac gttcctccgg
     1321 tggtcgcacg agcctgcaaa ggggcggagc tgtgcgcgtc cgacaagacg ctcaaagtca
     1381 tcgcgacggc gggaccggcc gtcgagccca tgcaatacag ggtgtgcgcc gccaaccgcg
     1441 ccggacgggc gtgctcggag ccggtgtcac cgaatttcag cgtgcaaagg atcgttccca
     1501 cgccacccgc gccgagcgtc gtcaaagtcc agccgccacc gccctcgaat gcgcacctgg
     1561 cctgcatcat cggcaaggat cccagcgacc ccacgcgaca gtgcgcggta tccgaaaaag
     1621 cgctcggggc cacctgcaag tgcccgagga tttacggcga gggtcgcgtg ggatggagta
     1681 cttatatccc gcaggagaaa aggcgcgctc tgggctttgg ccgatgacgc tcgcaggctg
     1741 attcgagact atttgccgct acggccagtg ccgtagggca caagtctgca gtgccgcgac
     1801 tcacatcggg ggccgtgccc tcgcgcctac agtgatcggc gcggaggtgc atcgacatgc
     1861 cgattttcga agtcacctac aaagcggtct gcccgggctg cgggcatcat tggaaaacca
     1921 ccgcgcgccg ggaagccaac gacaagatcg acgccagcga gtccgcgccg gcgatgcagt
     1981 gcgaaagctg caaggccttc acggtgccgt accgcgtgat cgtcggcgcg gccgggggag
     2041 cgacggcaac gatcgcgcgc tgaccggatg aagcggtgtg actctaatgg cgtccccacg
     2101 gggattcgaa ccccggttac cgccgtgaaa gggcgatgtc ctaggcctct agacgatagg
     2161 gactaaaggg cttcaacagc accagcagtt ggtggaggta agcgggatcg aaccgctgac
     2221 ctcttgcatg ccatgcaagc gctctcccag ctgagctata cccccaacag agccgcgtat
     2281 tctaccggcg ccgtcgcccc attgtaaata catcggggcg ctaaagcctt gtttcggcga
     2341 tcgacttctt attagcgcgg ccatcggcgg ggcgcggacg agcgcccggg cgcgactatg
     2401 acgacgtcgt agaacggcta cagccgaggc ggctttcggt gggcacggat gaaccattcg
     2461 gctcacgaac catcccagac attgtggtca taaatgcatc gctcgacccc gagaattgcc
     2521 gttgtcccga gcctggaagg gggctttggg cggggcttcg ccgggcgtgg tattccggac
     2581 gatgaaaatg catgtgacca gactcgagag aggccgtatg aagtccatcc ttgaccgttc
     2641 gttccgctat acgcccagcg tcgagaccga cctgcgcaag accttcgcca gaatccgccg
     2701 caagcagcgc gacaaagtgc gcgcgatcgc caacgcagac gccgaggtga agaccaaggt
     2761 gctgctgatc gatcatctga agaaagcctc gaacgccgcc ttcgccaagc cggtggagca
     2821 acgcggcatc gcgctcgacg acgtcggcgc cgtcccgaac cggggcaagt aagggccgtc
     2881 atctccgtct ttcgcgcatc cggccgccgc cggctcgcgc tggcggcggc gttcctccta
     2941 tgggcgctcg cgtgccggac ggcgctcgcg ttcggcttcg acgacgtcgc gcagcgcgcg
     3001 aaggcgctcg ccgacgcgcc gttcaagaag ggcgccgggc ttccgaagga gctgcagggc
     3061 ctcgactacg accattaccg agacatccgc ttccggcccg accgcttcct gtggcgcggc
     3121 acgagcagcc cgttcgagct cgccttcttc cacctcggcc tctactacga ccaggcggtg
     3181 aggatcaacg aggtgagcgc gtcgggcgtg cgcgagatcc cgttcgaccc cgacgacttc
     3241 gactacggct cgaacaagct cgacgtcgcg aagctcaagg aggagaaagg cctgggcttc
     3301 gccggcttcc gcgcgcacta cgcgctgaac actccgaaat acaaggacga ggtcgcggtg
     3361 ttcctcggcg cgagctattt ccgcgccctg gggaaaggcc agatctacgg tctttcggcg
     3421 cgcggcctcg cgatcgatac ggcgctgccc tcgggcgagg agttcccgcg cttcaccgag
     3481 ttctggatcg agcgcccggc ggcgggcgcg aaggagctcg tcgtgtacgc gctgctcgac
     3541 tcgcgccgcg ccgcgggcgc gtaccgcttc gtcgtgcagc ccggcgtgtc gacggcggtc
     3601 gaggtgaagg cgcgcctctt cctgcgcgag agcgtcggca agctcggcat cgcgccgctc
     3661 acgagcatgt tcttcttcgg cgagaaccag cgcccgccct acgacgacta ccggcccgag
     3721 gtgcacgact ccgacggcct gcaggtgcgc acgggcgcgg gcgagtggat ctggcggccg
     3781 ctcgtgaacc cgaagcggct cctcgtcacg tcgttcgcga cgaccgatcc gggcggcttc
     3841 ggcctgatgc agcgcgaccg cgctttcgcg cactacgagg acaccgaggt gcggcacgag
     3901 ctgcgcccga gcgtctgggt cgagccgaag ggcaagtggg gcgcgggacg cgtcgagctg
     3961 gtcgagatcc ccgcgcccga cgagaccaac gacaacatcg tcgcctactg ggtgcccgac
     4021 gcggcgccga agcccaagga ggcgctcgac ctcgagtacc ggctgctgtg gcagaaggac
     4081 tcggagacgc gtccgccgct cgcgcggacc gtgcagagcc ggcgcggccg cggctacatg
     4141 cgcaatcccg acaacagcat cggcttcgtc gtcgacttcc agggcgcgcc gctccagacg
     4201 ctcgccgccg acgcca
//
LOCUS       DATDMW010000297        10102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_837592, whole genome shotgun sequence.
ACCESSION   DATDMW010000297 DATDMW010000000
VERSION     DATDMW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837592"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="asnB"
                     /locus_tag="VI319_12450"
     CDS             complement(<1..1055)
                     /gene="asnB"
                     /locus_tag="VI319_12450"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY6864698.1"
                     /translation="MCGITGFWARHGEGGAWLQDLGGAVRSLQQRGPDDSGTWVRDGG
                     DLAFGHTRLSILDLSPSGHQPMISPDRDAVMVFNGEVYNFAEIRRELESLGHGFQSTG
                     DSEVVLTALRHWGIAAVDRFIGMFAIALWQESTRRLWLIRDRMGVKPLYWSWDGRVLW
                     FGSELKALRAHRAFKPELDRTALGEYFQFGYISAPRSVYQKVHKLRPGHWLELRETGN
                     PVARCYWSALEPREPFTEPEAELEERLESLLIDAFRYRMVSDVPVGVFLSGGVDSSLV
                     ASILQKHSGRTVHTFTVGFSDPRYDESRWARQVAAHLGTLHTEHVLSPAQVPRILERW
                     ADVFDEPFGDSSGVPTFL"
     gene            complement(1066..2577)
                     /locus_tag="VI319_12455"
     CDS             complement(1066..2577)
                     /locus_tag="VI319_12455"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY6864699.1"
                     /translation="MSSSANAGPSALSVPAEARAAGPRLPSLDPDRGNAASTSAIVAA
                     LALAAVASGVAIAVGDVTATVLCAAVLAVAFILMDYRVGVVLLILLMPIEPSILFPHS
                     MFGVTGVNPLNVLMLSTVCSLGIRHGGRMQATPFVPRPLLWLYIVPFVAAGIMGSRHV
                     GDIPEYLIPLHQFSFTDQPTYLRDVVVKPLFLVLFALLIGRAVADSEKPERFITPMLI
                     SVWIMGLLALGSFLASDVSFMSLASRRIAGEVLGVSGLHANDLGRLYAIAYALMLWPW
                     AMTSDHRLKFMLAITMGVVVLALLSTFSRGAFFSFFVVNIIFLFSRRSALAFIFAILL
                     VALAVPLAPGGLVYRMSEGLSGGHAGLNDFTSGRTDTIWLPLLPEIWKSPIVGNGLSS
                     TLWSDAMREMKMIVVAHPHNAFLGLLLDMGIVGLVTVCAYFVLVWRGFRTLYRDTTLS
                     TEMRALFRGAAAGLLAFATAGFAGSSLTPVPEQSYLWLAIGLMYGVLARKAKA"
     gene            complement(2592..3479)
                     /gene="epsG"
                     /locus_tag="VI319_12460"
     CDS             complement(2592..3479)
                     /gene="epsG"
                     /locus_tag="VI319_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HEY6864700.1"
                     /translation="MNLQENVLPIESGRIIGQQDRSLGAILIEEGKLSSVDIERILEL
                     QRMEGLRFGEAALRLGLITAQDLSAALAKQYDVPHVTADGDASSKELIAAYQPFHRRV
                     EELRALRTQLLIRWYNPDAGRKVLAVVSPGPGEGRTYVAANLALVFAQLGERTLLIDA
                     DMRAPRQHRLFNVPDRMGLSAALSGRADRSAVVACPDYGRLWVLPAGALPPNPQELLA
                     RHALGILLKELQADFDMVIIDTPPAKLYADAQAVAFRAQNAMVLARKNETRLADTTTL
                     VREFGDTGTHVVGTVINDF"
     gene            complement(3523..4935)
                     /gene="epsF"
                     /locus_tag="VI319_12465"
     CDS             complement(3523..4935)
                     /gene="epsF"
                     /locus_tag="VI319_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HEY6864701.1"
                     /translation="MNFYLLLSALRARAGVFALVLLATILVTAVVSVLLPKKYVATAT
                     LLADMRDEQSLTVPTTLGSQAGYLQTQVDILTSQKVARRVAQDLKLAEDKQARADWQS
                     DTGGKGSFDDWLADQLLKNLKVDTGGGRTVQVMYTAPDPAFAATAANAFAKAYMDTIL
                     ELRVAPTRQAAVWFDDQLKVLRNNLEQAKGRLAKYQSDKNIVVTDERMDTDNTRLMEL
                     SAELSRVQAQAVDVTSREKQAREFLAQGVAADKIPDIIANPFIQGMKTELLRGEARLS
                     ELSTRYGPNYPAVQTQVAENADLRQRLQVEMNKIVDGLQNQVRQLGRREQELRDALNA
                     QRTRLLQARSGRNELSAYMGDVDTAQRAYDAALQRSTVSKIESRASQTNVALLNPASA
                     PLDPSKPKVGLNILLSAIVGIMLGIAVVFLLETVDRRVRSYDDLYVGASVPVLAVLHA
                     QAPAGYRLALAPGYALPKPA"
     gene            complement(4988..5818)
                     /gene="epsE"
                     /locus_tag="VI319_12470"
     CDS             complement(4988..5818)
                     /gene="epsE"
                     /locus_tag="VI319_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HEY6864702.1"
                     /translation="MITPSISFSLPKLARAALAALGAAILAVSLPAAAQATDTIGEGD
                     TVHVTVFQNPDLSVDGRVAADGSIPYPLLGQVKIGGLTTSAAGGRIADSLKRGGYLKN
                     PQVTVAVTANRSRQVSVLGHVARPGRYALDDTTSRLTDVLALAGGIAPTGDDIVQVLV
                     NRGGKIAKLSVDVPVMFRSGDLSKNIEVHSGDTIFVSRAPVFYIYGEVLHAGAFRLEQ
                     NMSVMQALSVGGGLTPRGTERGLKINRLMPDGSVRTIDAKLTDLVQVDDVIYVKESIF
                     "
     gene            complement(5932..7071)
                     /locus_tag="VI319_12475"
     CDS             complement(5932..7071)
                     /locus_tag="VI319_12475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6864703.1"
                     /translation="MIRIEEVRDWSALETHADAWNALAGASATATVFQSYEWHDAWWR
                     VFADDYELRVLLAREGGRTVAIAPWAVSRSGRELLFIGSGDYASDYCDFLVDPAHPEA
                     VDAFAEWMFANRAAWRRVDLRNVPSGSPHRARLEARVRERSPWLFSEVEADAPTRLLG
                     DAAADRGAVNKSSLKRHFKQLSKAGRLSFEHLDAADETEPLLEAFFAQHVERRALAGG
                     KSQFLDPRQRRFYVELVRRLGPRGWLRFAVVRLDGEPIAFHLGFEYRSCFVWYKPAFS
                     VAHARRSPGEVLLKYLLEDAIARGLAEFDFTVGNEPFKYRFANRVRTNHRVRVYALPA
                     GYWLRRARGLAKRLLRRSPARPAAGADAPLGAACASRRPSLSRAD"
     gene            complement(7091..8356)
                     /gene="epsL"
                     /locus_tag="VI319_12480"
     CDS             complement(7091..8356)
                     /gene="epsL"
                     /locus_tag="VI319_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated polysaccharide
                     biosynthesis outer membrane protein EpsL"
                     /protein_id="HEY6864704.1"
                     /translation="MSNTRLQLKHLPPPWLAATALAAALLLSAGDAFALLGDRLELFA
                     QERVTYDDNVFRIARGVDPRPITGSQSKSDVYTTTSLGANLRLPVSRQLFDVGWAWNI
                     VRFNKFDDLDLTGHEGRAIWHWALGNDLSGAAGYTDTKAQAAFAGLGARVFDPVTTRT
                     AYANAVYNLTPSWRLNGGVAETHQRHQVDVRKVNDVNIRTTNAGFAYVTAAGNQLGVL
                     ADREEGEYPNRNFVPGLSIIDNNYHQNGAGVFTEYQLTGESHLSARVERIRRYYDNLP
                     QRDFSATVGRLAHDWAYSGRLTFSTILRREVVPVDDLASNVALARGITFAPTYRLTEK
                     ATLLGSFDWVRRRYLQDPGITTSDALVVAARPAGSEDRLWVASASIAYRYSRTLTFLT
                     TYSHEDRTATYTLGDYKANVVFVQARVGI"
     gene            complement(8533..9393)
                     /gene="epsA"
                     /locus_tag="VI319_12485"
     CDS             complement(8533..9393)
                     /gene="epsA"
                     /locus_tag="VI319_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated transcriptional
                     regulator EpsA"
                     /protein_id="HEY6864705.1"
                     /translation="MPEDHRTDSPELHRSESPEGITSPHVEEAGTTAGAMLLDSLELE
                     ALVLNLDASLNVHARHQFFTWTQGLLQSLIKHELLICALRNGEPMSFYVDAFSTFATE
                     PSLFSEVFRQDTSLVPHLIKAWEDNHYRPVLVDTGSGSFAGSPLARELNRVGASSLVA
                     HGTYDSTGKLASFFVFACQPGTIGPRQAHLVELVVPFLHGSWLRTQINRPVEHSSSKQ
                     GGVGLLTMREQEILKWIYLGKSNIEIGAILEISPLTVKNHVQKILRKLNVQNRTQAVG
                     KGLALRILST"
     gene            9835..>10102
                     /locus_tag="VI319_12490"
     CDS             9835..>10102
                     /locus_tag="VI319_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864706.1"
                     /translation="MRRITMSRKEMIRKLVALSVETALAESHRYWLSEVFEKGFVGYG
                     NFSDGQLLREMQLRGLAGPNDVSEEDDVDDGPSLDALDRTLPDLG"
BASE COUNT         1561 a         3319 c         3678 g         1544 t
ORIGIN      
        1 aggaaggtcg gcacgcccga ggagtcgccg aagggctcgt cgaagacgtc ggcccagcgc
       61 tcgaggatcc gcgggacctg cgccggcgac agcacgtgct cggtgtggag cgtgccgagg
      121 tgggcggcga cctggcgcgc ccagcgcgac tcgtcgtagc gcggatcgga gaatccgacc
      181 gtgaaggtgt gaaccgtgcg gccggagtgc ttctgcagga tcgaggcgac gagcgaggag
      241 tcgacgccgc ccgagaggaa aacgccgacc ggcacgtccg agaccatgcg gtagcggaag
      301 gcgtcgatca gcagcgactc gaggcgctcc tcgagctccg cttcgggctc ggtgaacggc
      361 tcgcgcggct cgagggccga ccagtagcag cgcgcgaccg ggttgccggt ctcgcgcagc
      421 tcgagccagt ggccgggccg cagcttgtgc accttctggt agacgctgcg cggagcggag
      481 atgtagccga actggaagta ctcgccgagc gcagtgcggt cgagctcggg cttgaacgcg
      541 cggtgcgcgc gcagcgcctt cagctccgag ccgaaccaga gcacccggcc gtcccaggac
      601 cagtacaggg gcttcacccc catgcggtcg cggatcagcc acaggcgccg cgtgctctcc
      661 tgccacagcg cgatcgcgaa catgccgatg aagcggtcga ccgcggcgat gccccagtgg
      721 cgcagcgccg tcagcacgac ctcggagtcg cccgtgctct ggaagccgtg ccccagcgac
      781 tcgagctcgc ggcggatctc ggcgaagttg tagacctcgc cgttgaacac catgaccgcg
      841 tcgcggtcgg gcgagatcat gggctggtgc ccggaagggc tcaggtcgag gatcgacagg
      901 cgcgtgtgcc cgaaggcgag gtcgccgccg tcgcgcaccc aggtgccgct gtcgtcgggg
      961 ccgcgctgct gcaggctgcg taccgcgccg cccaggtcct gcagccacgc gccgccctcg
     1021 ccgtgtcgcg cccagaatcc ggtgatcccg cacatggccg gccctctagg ccttcgcctt
     1081 tcgcgcgagc accccgtaca tcaatccgat cgccagccag agatacgatt gctcgggcac
     1141 cggcgtgagg ctgctgccgg cgaagccggc ggtcgcgaac gcgagcaggc ccgcggcggc
     1201 gccccggaac agcgcgcgca tctcggtgct gagcgtggtg tcgcgataga gcgtccggaa
     1261 gccgcgccac accagcacga agtacgcgca caccgtgacc aggccgacga tgcccatgtc
     1321 gagcagcagg ccgaggaagg cgttgtgcgg gtgcgcgacc acgatcatct tcatctcgcg
     1381 catggcgtcg gaccacagcg tggacgacag accgttgccg acgatggggc tcttccagat
     1441 ctcgggcagc agcggcagcc agatcgtgtc ggtgcgcccg gaggtgaagt cgttcagccc
     1501 ggcgtggccg ccgctcaggc cctcggacat gcggtagacc agcccgcccg gcgcgagcgg
     1561 gaccgccagc gcgaccagca ggatggcgaa gatgaaggcg agcgcgctgc gccgcgagaa
     1621 caggaagatg atgttgacga cgaagaagct gaagaacgcg ccgcgcgaga acgtcgacag
     1681 gagcgcgagc acgaccacgc ccatcgtgat cgcgagcatg aacttcagcc ggtggtccga
     1741 cgtcatggcc cacggccaca gcatcaacgc gtacgcgatc gcgtagagcc gtccgaggtc
     1801 gttggcgtgc agccccgaga cgccgagcac ttcgccggcg atcctgcggc tggcgagcga
     1861 catgaagctc acgtccgagg cgaggaagga gccgagcgcg agcaggccca tgatccagac
     1921 cgagatcagc atcggcgtga tgaagcgctc gggcttctcc gagtccgcga ccgcgcggcc
     1981 gatgaggagc gcgaacagca ccaggaacag cggcttcacg acgacgtcgc ggaggtaggt
     2041 cggctggtcg gtgaagctga actggtgcag cgggatgagg tactcgggaa tgtcgccgac
     2101 gtggcgcgat cccatgatgc cggcggcgac gaagggcacg atgtacagcc acagcagcgg
     2161 ccgcggcacg aacggcgtcg cctgcatgcg gcctccgtgc cggatgccga gcgagcacac
     2221 cgtcgagagc atcagcacgt tcagcggatt gacgccggtc accccgaaca tcgagtgagg
     2281 gaacaggatg ctcggctcga tcggcatgag caggatcagc aggaccacgc cgacgcggta
     2341 gtccatgagg atgaaggcga cggcgagcac cgcggcgcac agcaccgtcg cggtgacgtc
     2401 gccgacggcg atcgccacgc ccgaggcgac cgccgcgagc gcgagggcgg cgacgatcgc
     2461 cgacgtcgat gcggcgttcc cgcggtcggg atcgagcgag gggagccgcg gacccgcggc
     2521 gcgagcctca gccgggacgg acagcgcgga cggccccgcg ttcgcggacg acgacatgcg
     2581 acgctcccgg ctcagaaatc attgatgacc gtgccgacca cgtgcgtgcc ggtgtcgccg
     2641 aactcccgca ccagggtcgt ggtgtcggcg agccgcgtct cgttcttgcg cgcgagcacc
     2701 atggcgttct gcgcgcggaa tgcgaccgcc tgcgcgtcgg cgtacagctt cgccggcggc
     2761 gtgtcgatga tgaccatgtc gaagtcggcc tgcagctcct tgagcaggat gccgagcgcg
     2821 tggcgcgcga gcagttcctg cgggttgggc ggcaacgcgc ccgcgggcag cacccagagt
     2881 cgcccgtagt cggggcacgc cacgaccgcg ctgcggtcgg cgcggcccga gagcgccgcg
     2941 gacagcccca tgcggtcggg cacgttgaac agccggtgtt ggcgcggcgc gcgcatgtcg
     3001 gcgtcgatga gcagcgtgcg ctcgccgagc tgcgcgaaca cgagcgcgag gttcgcggcg
     3061 acgtaggtgc gcccctcgcc cggcccggga ctcacgaccg cgagcacctt gcggccggca
     3121 tccgggttgt accagcggat cagcagctgg gtgcgcagcg cgcgcagctc ctcgacgcgc
     3181 cggtggaacg gctggtaggc ggcgatcagc tccttgctcg acgcgtcgcc gtccgcggtc
     3241 acgtgcggga cgtcgtactg cttggcgagc gcggccgaca ggtcctgagc ggtgatcagc
     3301 ccgaggcgca gcgccgcctc gccgaagcgc aggccctcca tgcgctgcag ctcgaggatg
     3361 cgctcgatgt cgaccgagct cagcttgcct tcctcgatga ggatggcgcc gagactgcgg
     3421 tcctgctggc cgatgatgcg gcccgattcg atcggaagca cgttttcctg gaggttcatg
     3481 gcgtcctcga ttgatattcg aatgcgaacg tgggggccgc gcttacgccg gcttcggcag
     3541 cgcgtacccg ggggcgagcg cgagccggta gccggccggc gcctgcgcgt gcagcacggc
     3601 gagcaccggg acgctcgcgc cgacgtacag gtcgtcgtag ctgcgcacgc ggcggtcgac
     3661 ggtctcgagc aggaacacga ccgcgatgcc gagcatgatg ccgacgatgg ccgacagcag
     3721 gatgttgagc ccgaccttgg gcttcgacgg gtcgagcggc gccgacgcgg ggttgagcag
     3781 cgcgacgttg gtctggctcg cgcggctctc gatcttgctg acggtggagc gctgcagcgc
     3841 cgcgtcgtag gcgcgctgcg ccgtgtcgac gtcgcccatg tacgccgaca gctcgttgcg
     3901 cccggagcgc gcctgcagca gccgggtgcg ctgcgcgttg agcgcgtcgc gcagctcctg
     3961 ctcgcgccgg ccgagctggc gcacctggtt ctgcaggccg tcgacgatct tgttcatctc
     4021 gacctgcagc cgctggcgca ggtccgcgtt ctccgcgacc tgcgtctgca ccgcgggata
     4081 gttcgggccg tagcgggtgg agagctccga gagccgcgcc tcgccgcgca gcagctcggt
     4141 cttcatgccc tggatgaagg gattggcgat gatgtccggg atcttgtcgg cggcgacgcc
     4201 ctgcgccagg aactcgcgcg cctgcttctc gcggctcgtg acgtcgacgg cctgggcctg
     4261 cacccgcgag agctcggcgg aaagctccat caggcgcgtg ttgtcggtgt ccatgcgctc
     4321 gtcggtgacg acgatgttct tgtcgctctg gtacttcgcg agccgcccct tcgcctgctc
     4381 caggttgttg cgcaggacct tgagctggtc gtcgaaccag accgcggcct gtcgcgtcgg
     4441 cgccacgcgc agctcgagga tggtgtccat gtacgccttg gcgaaggcgt tggcggcggt
     4501 cgcggcgaac gccggatccg gcgccgtata catcacctgc acggtgcggc cgccgccggt
     4561 gtcgaccttg aggttcttca gcagctggtc ggcgagccag tcgtcgaagg agcctttgcc
     4621 gcccgtgtcg gactgccagt cggcgcgcgc ctgcttgtcc tcggcgagct tgaggtcctg
     4681 cgccacgcgg cgcgcgacct tctggctggt caggatgtcg acctgggtct gcaggtagcc
     4741 ggcctggctg ccgagcgtcg tcggcacggt cagcgactgc tcgtcgcgca tgtcggcgag
     4801 cagcgtcgcc gtcgcgacgt atttcttggg cagcagcacg ctcacgacgg cggtgaccag
     4861 gatggtcgcg agcaggacga gcgcgaacac gccggcgcgc gcgcgcaatg ccgagagcag
     4921 gagatagaag ttcatgctgg gttccgatcg gttgagtctt ggggttcggc cggggacgtc
     4981 cgccgggcta gaaaatgctt tccttgacgt agatgacgtc gtccacctgg acgaggtccg
     5041 tcagcttcgc gtcgatggtg cgcaccgagc cgtcgggcat caagcggttg atcttgagcc
     5101 cgcgctcggt gccgcgaggc gtcagcccgc cgccgaccga gagcgcctgc atgacgctca
     5161 tgttctgctc gaggcggaag gcgcccgcgt gcagcacctc gccgtagatg tagaagacgg
     5221 gcgcgcgcga gacgaagatc gtgtcgccgc tgtgcacctc gatgttcttc gacaggtcgc
     5281 cgctcctgaa catgaccggc acgtcgaccg acagcttggc gatcttgccg ccgcggttga
     5341 cgagaacctg gacgatgtcg tcgcccgtcg gcgcgatgcc gccggcgagc gcgagcacgt
     5401 cggtcaatcg cgaggtggtg tcgtcgagcg cgtagcggcc ggggcgcgcg acgtggccga
     5461 gcaccgagac ctgccggctg cggttcgccg tcacggcgac cgtcacctgg gggttcttca
     5521 ggtagccgcc gcgcttgagc gagtcggcga tgcgcccgcc cgcggccgag gtcgtgagcc
     5581 cgccgatctt cacctgtccg agcagcggat acgggatcga gccgtcggcg gcgacgcggc
     5641 cgtcgacgct caggtcggga ttctggaaga ccgtcacgtg gacggtgtcg ccctcgccga
     5701 tcgtgtcggt cgcctgcgcc gccgcgggca gcgacacggc gaggatcgcg gcgccgagcg
     5761 cggcgagcgc cgcgcgcgcg agcttcggga gcgagaagga gatggaaggc gtgatcattg
     5821 aagtctccga aggttcggaa ttgaaactga tccgtttggc cgttgcggtc atggaagctg
     5881 caaccttagc gaccgaatcg gtctgtgaaa gcgtcgtcat gccggcctgc tctagtccgc
     5941 tcggctcagg gacggtcggc ggcttgcaca cgcagcgccg agcggcgcat ccgctccggc
     6001 ggccggccgc gccggcgagc gccgcaggag ccgcttcgcg agtccgcggg cgcggcgcag
     6061 ccagtacccg gccgggagcg cgtagacccg aacccggtgg ttggtgcgca cccggttcgc
     6121 gaagcggtac ttgaacggct cgttgccgac ggtgaagtcg aactcggcga gtccgcgggc
     6181 gatcgcgtcc tcgagcaggt atttgagcag cacctcgccg ggcgagcggc gcgcatgagc
     6241 cacgctgaac gcgggcttgt accagacgaa gcacgagcgg tactcgaacc cgaggtggaa
     6301 cgcgatcggc tcgccgtcga gccgcaccac ggcgaagcgc agccagccgc ggggcccgag
     6361 ccggcggacg agctcgacgt agaagcgcct ctggcgcgga tcgaggaact ggctcttgcc
     6421 cccggccagg gcgcgacgct cgacgtgctg cgcgaagaac gcctcgagca gcggctcggt
     6481 ctcgtccgcg gcgtccaggt gctcgaacga gagccggccg gccttcgaga gctgcttgaa
     6541 gtggcgcttg aggctcgact tgttcaccgc tcctcgatcg gcggccgcgt cgccgagcag
     6601 ccgcgtcggc gcatcggcct cgacctccga gaacagccac ggcgagcgct cgcgcacgcg
     6661 cgcctcgagc cgcgcccggt gcggggagcc gctggggacg ttgcgcaggt cgacgcggcg
     6721 ccaggccgcg cggttcgcga acatccactc ggcgaaggcg tcgaccgcct cggggtgcgc
     6781 cggatccacc aggaaatcgc agtagtcgga ggcgtagtcg cccgacccga tgaacaggag
     6841 ctcgcggccg ctgcggctca ccgcccaggg cgcgatcgcg accgtgcgcc cgccctcgcg
     6901 cgcgagcagc acgcgcagct cgtaatcgtc cgcgaacacg cgccaccagg cgtcgtgcca
     6961 ttcgtaggac tggaacacgg tcgcggtggc cgaggcgcct gcgagcgcgt tccaggcgtc
     7021 ggcgtgcgtc tcgagggcgc tccagtcgcg gacttcctcg atgcggatca tcgcgtccgc
     7081 gtccgggcca tcagatgccc acccgggcct ggacgaagac gacgttcgcc ttgtagtcgc
     7141 cgagggtgta cgtcgcggtg cggtcctcgt gcgagtacgt cgtgaggaat gtcagggtgc
     7201 ggctgtagcg gtaggcgatg ctcgccgagg cgacccacag ccggtcctcg ctgcccgcgg
     7261 gccgggccgc cacgacgagc gcgtcgctgg tcgtgatgcc gggatcctgc aggtagcggc
     7321 gcctcaccca gtcgaagctc ccgagcaggg tcgccttctc ggtgagccgg taggtgggcg
     7381 cgaacgtgat cccgcgcgcc agcgccacgt tcgaagccag gtcatcgacc ggaacgacct
     7441 cgcgccgcag gatcgtcgag aaggtcagcc ggccgctgta ggcccagtcg tgcgcgaggc
     7501 ggccgacggt cgcgctgaaa tcgcgctgcg gcaggttgtc gtaatagcgc cggatgcgct
     7561 cgacgcgcgc gctgaggtgc gattcgccgg tcagctggta ctcggtgaac acgccggcgc
     7621 cgttctggtg atagttattg tcgatgatgc tcaggcccgg gacgaagttg cggttcgggt
     7681 attcgccctc ctcgcggtcc gccagcacgc cgagctggtt gcccgccgcg gtgacgtagg
     7741 cgaacccggc gttggtcgtg cggatgttga cgtcgttgac cttgcgcacg tcgacctggt
     7801 ggcgctggtg cgtctcggcg accccgccgt tgaggcgcca gctgggcgtg aggttgtata
     7861 cggcgttggc gtaggcggtg cgcgtcgtga ccgggtcgaa tacgcgcgcg ccgaggccgg
     7921 cgaacgcggc ctgcgccttg gtgtcggtgt agccggccgc gcccgagaga tcgttgccga
     7981 gcgcccaatg ccagatcgcg cgcccctcgt gccccgtgag gtcgaggtcg tcgaacttgt
     8041 tgaagcggac gatgttccag gcccagccga cgtcgaacag ctggcggctg acgggcaggc
     8101 gcaggttcgc gccgagcgac gtcgtcgtat acacgtcgga cttcgactgc gagcccgtga
     8161 tcggccgcgg atcgacgccg cgcgcgatgc ggaacacgtt gtcgtcgtag gtcacccgct
     8221 cctgcgcgaa cagctcgagc cggtcgccca ggagcgcgaa ggcgtcgccg gcggagagca
     8281 gcagcgcggc tgccagcgcg gtcgcggcga gccacggcgg cggaaggtgc ttcagttgca
     8341 gtctggtatt ggacataggc cttttgcgtt gtttgagcgc ccgtccctgc gctgaaggac
     8401 ctaaggctat aggcgccaga agtcacgacc atggccatgc actaggaaag atggactacg
     8461 ccgcgcggcg cgggtgcgcg taaacgacaa agccgcgcgg gcgcgcggct tcgtcgtcgt
     8521 cgaacggcga cgtcaggtgc tcaggatccg cagcgcgagg cccttgccga cggcctgggt
     8581 gcggttctgc acgttgagct tccggaggat cttctgcacg tggttcttga ccgtgagcgg
     8641 actgatctcg aggatggcgc cgatctcgat gttgctcttg ccgaggtaga tccacttcag
     8701 gatctcctgc tcgcgcatcg tgagcagccc cacgccgccc tgcttcgagc tcgagtgctc
     8761 gaccgggcgg ttgatctggg tgcgcagcca cgagccgtgc aggaacggca cgaccagctc
     8821 cacgaggtgc gcctggcgcg gtccgatggt tcccggctgg caggcgaaca cgaagaagct
     8881 cgcgagcttg ccggtcgagt cgtaagtgcc gtgcgccacc aggctgctcg cgccgacgcg
     8941 gttgagctcg cgcgcgagcg ggctgccggc gaagctgccg ctgcccgtgt cgacgagcac
     9001 cggccggtag tggttgtctt cccacgcctt gatcaggtgc gggacgagcg acgtgtcctg
     9061 gcggaagacc tcgctgaaga gcgagggctc ggtcgcgaag gtggagaagg cgtcgacgta
     9121 gaacgacatc ggctcgccgt tgcgcagggc gcagatcagc agctcgtgct tgatcaggct
     9181 ctgcagcagc ccctgcgtcc aggtgaagaa ctggtgccgg gcgtgcacgt tgagggatgc
     9241 gtcgaggttg aggaccaggg cttcgagctc gagcgagtcg agcagcatcg ccccggcggt
     9301 cgtccccgcc tcttcgacgt gcggcgaagt gatgccctcc ggcgactcgg agcggtgcag
     9361 ctccggagaa tcggtccggt ggtcttccgg cacttcaggc cggcgttgcg cggttacttg
     9421 ttcgtcgcca gcctgcatga gcggcttcca gggtgagtca tcggggccaa tcatgacgac
     9481 cccatgttac aaaagcatga atgctcagaa tttaattccc ccgccgttca gtcgcccggc
     9541 ggcgacagcg cctttcgggg gtcgcggagg ccgggaaatt ttcccaggct cgcgcgccgg
     9601 cgcgcaacgc ggtgtctatg agatcttgcg gccgctggcg cgccgaataa gttcccggac
     9661 gcggcgggac gatgctcgca tcgtggaccg ctttcggagc aggcgcgcgg gaaaattgcg
     9721 gcgcgcacga tagtccggtc tacctagcgc ggacttccgc ggcttcatcg ttcgccaccg
     9781 catgccgtcc taggcttcgt catcgccgtt tcgcaccccg tggaggaaac cgcaatgcga
     9841 cgaatcacga tgtcgagaaa agaaatgatc cgcaagctgg tcgcgctgtc ggtggagacg
     9901 gcgctcgccg agtcgcaccg gtactggctg agcgaggtgt tcgagaaggg cttcgtcggc
     9961 tacggcaact tctccgacgg ccagctgctg cgcgagatgc agctgcgcgg cctcgccggc
    10021 ccgaacgacg tgtccgaaga ggacgacgtc gacgacggac cgtcgctcga cgcgctcgac
    10081 cgcactctcc cggatctcgg at
//
LOCUS       DATDMW010000298        20201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1835004, whole genome shotgun sequence.
ACCESSION   DATDMW010000298 DATDMW010000000
VERSION     DATDMW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20201
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835004"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VI319_12495"
     CDS             <1..1050
                     /locus_tag="VI319_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY6864707.1"
                     /translation="RVGITESEGARDEVRDQLRDSERAISATNRELRVLAAKRSALQA
                     QSRSLAQRRRQAESELGARRESLGRLLLRRYLDGGEGYMRLALSGRDPSQMARELHYY
                     GYLSEAQARMMERLRDLLLQTRDLERQLEEKRAELASVESDQRARRSDLAKEQAAKRV
                     VLARVSNRLREQRREVHGLERDEARLARLVQQIAKMLATTPGALRNEALPEAGAPERR
                     FATLKGALRLPARGEVTNRFGAARPGGGPMWKGLFIRAPAGAEVRAVAGGRVVFADWL
                     RGFGNLLILDHGDAYLTIYGNNESLLRQVGDVVRTGEVIATTGNSGGSQNSGIYFELR
                     HEGRPFDPLRWVSLR"
     gene            1135..2559
                     /locus_tag="VI319_12500"
     CDS             1135..2559
                     /locus_tag="VI319_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY6864708.1"
                     /translation="MRIRIKQVSLVLIGVLAGVLLSLNFSAVAQRDGTRYPLPVDELR
                     AFAEVFGAIKSSYVEPVEDKKLITEAISGMLTGLDPHSAYLDQDAFKELQVGTQGEFG
                     GLGIEVGMEDGFVKVVSPIEDTPAFRAGLKPGDLIIKLDDTPVKGMNLNDAVKRMRGK
                     PKTAIRLTIMRKGGDSKPFEVTLMRDIIRVQSVKSKLIEPGYGWIRVSQFQEHTGENL
                     VKALEGLYKQGPLKGLVLDLRNDPGGLLNGAVGIASAFLPARALIVSTDGRTEDAKRK
                     FLASPEDYLRGNREDYLKALPAGVKTVPMVVLVNGGSASASEIVAGALQDHKRATVLG
                     TQTFGKGSVQTILPLGNNTAIKLTTARYYTPSGRSIQAKGIEPDFVIEDPTSPTTGRL
                     REVDLDKHLLNEQDKSIEKLHRDEAARAAGKTGTDDNKPAAPLEFASDKDFQFQQALK
                     FLKGQPLTTAQSAAATAVPVVKKN"
     gene            2600..3391
                     /gene="moeB"
                     /locus_tag="VI319_12505"
     CDS             2600..3391
                     /gene="moeB"
                     /locus_tag="VI319_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY6864709.1"
                     /translation="MTDEQLLRYSRHILLAEFGVEAQEKLISASVLVIGAGGLGSPAA
                     LYLAAAGVGTLTLADGDAVDLTNLQRQILHTTRSVGTPKAESGKAALAQINPEVRVVP
                     VSRRLAGDELERLVAASDAVLDCSDNFETRHAVNRACVRHGRPLVSGAAVRFDGQVTV
                     FDLRDEASPCYSCLFPEDAELEETRCAVMGVFAPLTGIVGTIQAAEALKLITGVGTTL
                     AGRLLIIDALSMEWRSVKLARDPGCPVCATRAAATAYPSPPSATA"
     gene            complement(3339..3908)
                     /gene="slmA"
                     /locus_tag="VI319_12510"
     CDS             complement(3339..3908)
                     /gene="slmA"
                     /locus_tag="VI319_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010999998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEY6864710.1"
                     /translation="MPAKPGERRLQILKVLAQMLQDPKGEKITTAALAEKLDVSEAAL
                     YRHFASKAQMFEGLIEFIEQSLFGLVNKITAEEDDGIKQAHAILASLLGFAEKNPGMT
                     RVLIGDALVNEDERLQARINQLHDRLEATLKQCLRVAGTEAGAAQAEPGAHANLLLCY
                     VIGRWQQFAKSGFRRLPTEGWDKQWPLLV"
     gene            complement(3964..4620)
                     /locus_tag="VI319_12515"
     CDS             complement(3964..4620)
                     /locus_tag="VI319_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEY6864711.1"
                     /translation="MAANRLTWIFDLDNTLHDYRSRIMPHINRSMTEFLRRDLGLEEA
                     EADRLRRAYWLRYGATLVGMMRFHGTDPRRFLHETHHLPDLERLVLAERGLRHALRRI
                     PGRKLLFSNAPLAYAERILRALRLETSFDALYCIERARFHPKPSRRGFRVMIRAERLV
                     AERCVLVDDTVENLRTAKTLGMKTVWVSREVRVPLSVDVRVASVLALPRLADDLSKLS
                     "
     gene            4650..5123
                     /locus_tag="VI319_12520"
     CDS             4650..5123
                     /locus_tag="VI319_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEY6864712.1"
                     /translation="MARLSPRFSLKHLIVSETAARRGLDNRPPARVVRRLRRLAAGLE
                     RVRAITGRDLDVTSAYRGPALNALVGGSRASHHMQGLAADFSCPRFGSPFRICQAIVR
                     SPLRFDQLIYEHGDADDRGWVHLSFAPKMRRRVLTICKSRGTYQRGLRRCPVDLL"
     gene            complement(5115..6032)
                     /gene="argB"
                     /locus_tag="VI319_12525"
     CDS             complement(5115..6032)
                     /gene="argB"
                     /locus_tag="VI319_12525"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY6864713.1"
                     /translation="MTSATKGVVTGAITSEALTPAQKAKVLAEALPYIRRFHGRTIVV
                     KYGGNAMTDARLKESFAHDVVLLKLVGMNPVVVHGGGPQIDELLARVGKKGEFVQGMR
                     VTDAETMDIVEMVLGGQVNKEVVTLINQAGGRAVGLTGQDGAFIRARKMLLSDKSNGR
                     IDIGQVGEIASIDPSVISPLEQAGFIPVVAPIGVGDDGQSYNINADLVAGKLAEVLRA
                     EKLIVLTNTPGVLDKSGKLLTGLSPKKIDALVTDGTLSGGMLPKISSALDAARSGVPS
                     VHIIDGRVEHALLLEILTDEGVGTLIRSK"
     gene            complement(6114..6539)
                     /locus_tag="VI319_12530"
     CDS             complement(6114..6539)
                     /locus_tag="VI319_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6864714.1"
                     /translation="MASITRYDPFGDLVDDFFKGFFVRPVGFEAGEPVGRVKLEVTEA
                     NGEYKVLAELPGVKKEDIQVNIDGDHVSITAETRAERDVKDGERVIHSERQVGKVSRV
                     FRLAQEVDESKASARYIDGVLELTLPKKTAAAAKQLTIQ"
     gene            complement(6844..7287)
                     /locus_tag="VI319_12535"
     CDS             complement(6844..7287)
                     /locus_tag="VI319_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY6864715.1"
                     /translation="MVLARALGLSLLCALARPAAAQVHATDAWARGTVPGQSSSVVYM
                     RLRSDEPAAIVAVESPIGTAELHEMKMDGDIMRMRPLKRLELPAGRVVALDPSGAHVM
                     LTGLKRPLAKGDRVPLRLVVETAGGKRRRVDVSAEVRDFASGARR"
     gene            complement(7296..8648)
                     /gene="hslU"
                     /locus_tag="VI319_12540"
     CDS             complement(7296..8648)
                     /gene="hslU"
                     /locus_tag="VI319_12540"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY6864716.1"
                     /translation="MSSMTPQEIVHELDKHIIGQHRAKRSVAIALRNRWRRQQVGEPL
                     RQEITPKNILMIGPTGVGKTEIARRLARLADAPFVKVEATKFTEVGYVGRDVDTIIRD
                     LVEIAIKDAREAATRKVRRRAEDAAEERLLDVLLPGARRLDAFAAEVDAAPRTGEEES
                     ATRQKFRKRLREGELDDREVEIEIAAPQAHMEILAPPGMEELTQQIQGMFQNLGGARK
                     RPRKMKIREAMKILTDEEAARLVNDEDLRAKALANVEQNGIVFLDEIDKIASRGEMAG
                     ADVSRQGVQRDLLPLVEGTTVATKYGMVNTDHILFIASGAFHLSKPSDLIPELQGRFP
                     IRVELDSLSVSDFEQILTNTDACLTRQYQALLATEGVEVSFTPEGIRRLAEIAYAVNE
                     KTENIGARRLYTVMEKLLEEISFDAGKRGKESVVVDTAYVDARLQDLARSEDLARYVL
                     "
     gene            complement(8691..9227)
                     /gene="hslV"
                     /locus_tag="VI319_12545"
     CDS             complement(8691..9227)
                     /gene="hslV"
                     /locus_tag="VI319_12545"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY6864717.1"
                     /translation="MQQFHGTTILSVRRDGKVALGGDGQVTLGNVVVKSGARKVRRLY
                     HDRILAGFAGGTADAFTLFERFEAKLEKHQGNLLRSAVELAKDWRTDRMLRRLEAMLG
                     VADRESSLVITGMGDVLEPELGMISLGSGGPYAQSAARALLDNTTLSAEQIVRKALAI
                     AADICIYTNQNTVVEVLE"
     gene            complement(9317..10426)
                     /locus_tag="VI319_12550"
     CDS             complement(9317..10426)
                     /locus_tag="VI319_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6864718.1"
                     /translation="MPKRELATAAARASAPQAAVPARAPREPDSVMSIEVTAASGDVA
                     PVIEETAILFANGQAEQALAKLSQAVRGEDLGASAVQCWLMLFDVYQHLGRREDYESL
                     ALEFAVKFERSAPAWRDGGVEARANPALLTGGTGYCAFAGSIAAGAGEFEKLANLAQR
                     QQMIRVDFGRMEAIETAAAERLRAALVDLRSGGKEVVLTGEARLLELLEGACSAGNAG
                     TDRAMWALLFEVYKWFGRQEAFEEAAVNYAVTYEVSPPSWEQRPPAATKAPVARSVGD
                     SPATALTLAGDVTSAGEALVKDLQDWAAANSMLVVDMSKARRVDFVSAGVMLNALSQL
                     RQAGTTIQIRGANEMIAALFEVMGVSKVATVIRRK"
     gene            complement(10704..11465)
                     /locus_tag="VI319_12555"
     CDS             complement(10704..11465)
                     /locus_tag="VI319_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY6864719.1"
                     /translation="MNLDAASFGILWPAFVAGLLVTATHVPLGMQVLQRGIVFIDLAV
                     AQVAGLGVILADFLGFEPTGSAVQIAALGAALACAMLLTWTEKRWPEVQEAIIGVVFV
                     VGSSVALLLLAKNPRGSENLRELLIGQILWVNPSMLPLIALVSAGILGVWFTLRERLG
                     RIGFYAVFAVAVTMSVQLAGLYLVFATLVVPALSTYYDPRKRYLKAYAVGVLGYAVGL
                     VVSAVADLPSGAMIVCAIVAIGAVVAALGRRRSAA"
     gene            complement(11462..12367)
                     /locus_tag="VI319_12560"
     CDS             complement(11462..12367)
                     /locus_tag="VI319_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY6864720.1"
                     /translation="MKILLKTALFLLSLALPWSASAALNVFTCEPEWASLAQEIGGDK
                     VSVTSATTARQDPHRLEARPSLIARIRRADLLVCTGAELEVGWLPLLLTQSGNPRIQS
                     GSPGYVEASQFVPKLEVPKVLDRSLGDIHPSGNPHVHLDPRNIAKVAQVLAERMARVD
                     AGNAAFYRDRAADFGRRWASAMQRWDRDKERLKGVAIVVYHRDFSYFINWAGMREVGS
                     LEPKPGIPPSPSHLAELVEQMKRDPAKVVVYSPYNDPRAAEFLSQRAGIPTTMMPFTV
                     GGTDGAKDLFGLFDDTLARLLAVIK"
     gene            complement(12378..13742)
                     /locus_tag="VI319_12565"
     CDS             complement(12378..13742)
                     /locus_tag="VI319_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864721.1"
                     /translation="MQKTSTLALAVAAAVGIAGPGFAQTNDPLRDEVRQLKERTDALE
                     KRVQDAESAANQAAQAGNRPAGENALNPAVSLILNGVYSSLSRSPGDFRINGFVPTLG
                     EVEPFPRGPSLGESELALAANIDPDFRGTGIFSITPQNEVAAEEAYIQTLALSHGFTV
                     KAGRFFSGVGYQNQIHAHAWDFTDAPLANKVFLGNQLADDGVQLKWVAPTDTYFDLGL
                     ELGRGRAFPAAAPEGAARDKNGVGSANAFGHLGGDFGSGFAWQAGLSYLRTSPRDRTF
                     QDADSAGIAVTNAFSGRSRLWALSGVLKWAPDFNSTETSVKLQGEYFRRKEDGDLTFD
                     TAAAAAGPQTGAFASRQSGWYLQTVYQFLPMWRVGYRYDRLSAGTQTIGLVDSGALTP
                     ADFPVLGHYNPTRNTVMADWSASEFSRVRLQLARDRSRQNEPDNQVFLQYIVSLGAHG
                     AHTF"
     gene            complement(13824..14123)
                     /locus_tag="VI319_12570"
     CDS             complement(13824..14123)
                     /locus_tag="VI319_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864722.1"
                     /translation="MSRTLRYVLIAAALIFAQQSAQLHALSHVPHDPAKKGMPFSGHP
                     AAQCLAFHAVDSVLPNAVRAVKPARVAPPGVSYFVLPLPFQSRIEFDSRAPPVFS"
     gene            complement(14198..14557)
                     /gene="dksA"
                     /locus_tag="VI319_12575"
     CDS             complement(14198..14557)
                     /gene="dksA"
                     /locus_tag="VI319_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY6864723.1"
                     /translation="MNPQQLAFFRERLLEMQRELLENADVTSERLREHELEPDPTDQA
                     TIEEEYALELRTRDRERKLLKKIEQALRRIEDGSYGYCEETGEAIGIPRLLARPTATL
                     TIEAQARRELKQKLYGE"
     gene            complement(14674..15666)
                     /locus_tag="VI319_12580"
     CDS             complement(14674..15666)
                     /locus_tag="VI319_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6864724.1"
                     /translation="MATEDNMVPVTILTGFLGSGKTTLINRILKEQHGHRIAVIENEF
                     GEAGVDNEILLENEGEQIVEMNNGCVCCTVRGDLIRILGELKDRRESGKLKFERVIIE
                     TTGMADPGPVAQTFFVDEHVGNYYLLDGVITVVDAKHGPKQLDEFHEAQEQVGFADRI
                     LLSKTDLVPEDEQSRLRKRLVQMNPRAPIKNVHFGNAPIDEILDIRGFNLNAILELDP
                     EFLEDDEHEHDEQVSSFVFRSDKPFDGVRLEEFLSGLIQVYGPNMLRYKGVLYLKGNT
                     NRVVFQGVHMLMGGDVGRPWGRDEKRSSTLVFIGKNLPQDLFIKGLEQCLEATQ"
     gene            complement(15847..17082)
                     /locus_tag="VI319_12585"
     CDS             complement(15847..17082)
                     /locus_tag="VI319_12585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY6864725.1"
                     /translation="MPRTRAPRERTERAAGKLVLKPGREKSLLRRHPWVFSGAVERAE
                     NTGDAGETVKIVTNDGRFLAWGAFNPSSQIRARVWSFEEPRPPGPDLIREKIGAALAL
                     RRARIGARINVDTDALRLVHAESDGLPGVIADRYAATLVVQLLSAGAERWRSTLVAQL
                     AELSGCERVYERSDAEARQLEGLAARDGWASGAAGEPVVEIREHGLRYAVDVAHGQKT
                     GFYLDQRDNRARVGELSRDREVLNCFCYTGGFTLSALAGGARSVLSVDSSAAVLRAAQ
                     ANAERNGLAAAAQWLQGDVFRMLRELNAQQRRFDLIVLDPPKFAPTSKDAERAARGYK
                     DINLNALRLLRPGGLLATFSCSGGVSPELFQKIVAGAAADAPASLLLRERRWAAPDHP
                     VLVEFPEGEYLKGLLLERV"
     gene            complement(17048..17959)
                     /gene="xerC"
                     /locus_tag="VI319_12590"
     CDS             complement(17048..17959)
                     /gene="xerC"
                     /locus_tag="VI319_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY6864726.1"
                     /translation="MPAPDGDAKRELLAAFLRSLESGRRLSPHTRSGYARDIERLLEE
                     IDDAPLDQVSSHLVRRAVARLHARGLSGKSLARALSAWRSFFAWLARHRGLGANPCAG
                     IRAPKSAKALPRVLSPDQAAQLLDRRPDDDRETRDKAMFELFYSSGLRLAELVRLDLE
                     DARTAIEEAELAVLGKGNKTRRVPVGRKAIEALRAWLSIRPALARAGERALFTGARGR
                     RIAPRVVQHRLARWAKKLGIAAPVHPHVLRHSFASHLLQSSGDLRAVQDLLGHSSIST
                     TQVYTHLDFQHLAKAYDAAHPRAKRKN"
     gene            complement(17971..18618)
                     /locus_tag="VI319_12595"
     CDS             complement(17971..18618)
                     /locus_tag="VI319_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY6864727.1"
                     /translation="MRPDDVARYLQENPVFFEQYADLLSQIYIPHPHGGRAIPLSERQ
                     VLTLRERARALESRLAELLEVGEENDAIGEKMHRLCLALLPVPDLRALLSALDYNLRE
                     DFAVPHVALRVWREGAAAELAELGGASDDAKHYAAGLAQPFCGPAGNSEVVAWFGEAA
                     AHVRSVASVPLREKGECFGMLVLGSEDAARFYPEMGTVYLKRLGELASASLLRFL"
     gene            complement(18608..19447)
                     /gene="dapF"
                     /locus_tag="VI319_12600"
     CDS             complement(18608..19447)
                     /gene="dapF"
                     /locus_tag="VI319_12600"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY6864728.1"
                     /translation="MKLKFAKMQGLGNDFVVIDGVRQRVAMTPALARRLADRHFGIGC
                     DQVLLVEKPGSAGADFRYRIWNADGGEVGQCGNGARCFVRFVRDHALSDKRELKVETA
                     SGMIVPRLEADGEVTVDMGSPRFDPASIPFLSQSPAPVQPLELPTGRTVQITAVSMGN
                     PHAVQLVDDVDSAPVTTEGPVIEKHPRFPERVNAGYMQVLGRGRIALRVWERGAGETL
                     ACGTGACAAVAAGIRRGLLDARVRVATRGGDLTIEWRGGDNPVWMSGPAVTVFEGEIN
                     VET"
     gene            complement(19470..>20201)
                     /locus_tag="VI319_12605"
     CDS             complement(19470..>20201)
                     /locus_tag="VI319_12605"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEY6864729.1"
                     /translation="LERCFPGLPPGERVRIARRHFGAFVRSVLERGILWWAPRERIVR
                     LVRIVGMEHIHAAKDAPVLLLAPHFVGLDAGCTRLTCEIDMAGIYAKQKDAVFDAMLL
                     HGRTRFGHQRAISRQEGVRAAIAVLKDHVPFYYLPDQDYGARESIFVPFFGVPAATVT
                     GLSRLARLVGARVIPCVTRMLPGGAGYEVRCYPAWEDFPGADPEADARRMNAFIEERV
                     LEMPEQYFWTHKRFKTRPPGEPRWY"
BASE COUNT         3164 a         6975 c         6840 g         3222 t
ORIGIN      
        1 cgcgtcggca tcaccgaatc cgaaggcgca cgtgacgagg tccgcgacca gctgcgcgac
       61 tccgagcgcg cgatctccgc gaccaaccgc gagctgcgcg tcctggcggc gaagcgcagc
      121 gcgctgcagg cgcagtcgcg ctcgctcgcg cagcgacggc gccaagcgga atccgagctc
      181 ggcgcgcgcc gcgaatcgct cgggcggctg ctcctgcggc gctatctcga tggcggcgag
      241 ggctacatgc ggctcgcgct ctcgggccgc gatccgtcgc agatggcgcg cgagctgcac
      301 tattacggct atctctccga ggctcaggcc cgcatgatgg agcgcctgcg ggacctgctg
      361 ctccagacgc gcgacctcga gcgccagctc gaggagaagc gggcggagct cgcgtccgtg
      421 gaatcggatc agcgggcccg ccgcagcgat ctcgcgaagg agcaggccgc caagcgcgtc
      481 gtgctggcgc gcgtctccaa caggctgcgc gagcagcgcc gcgaggtgca tggcctcgaa
      541 cgcgacgagg cgcggctcgc gcgactcgtg cagcagatcg cgaagatgct cgcgaccact
      601 cccggcgcgc tgcgcaacga ggcgcttccg gaggcgggcg cgcctgagcg gcggttcgcg
      661 accctgaagg gcgcgctgcg gctgcccgcc cggggcgagg tgaccaaccg cttcggcgcc
      721 gcccggccgg gcggcgggcc gatgtggaag ggtcttttca tccgagcacc cgcgggcgcg
      781 gaggtgcgcg ccgtcgccgg cggtcgcgtg gtgttcgccg attggctccg cgggttcgga
      841 aatctgctga tcctcgacca cggcgatgcg tatctgacga tctatggaaa caatgaatcg
      901 ctattgcggc aggttggtga cgtagtgcgc acaggtgaag tcatcgcgac cactggaaac
      961 agcggcggtt cccaaaattc cgggatatac tttgagctca ggcatgaagg acgacctttc
     1021 gatcccctgc gctgggtttc gttgaggtag ggctgcaccc tcccgcgctt catgctcttg
     1081 tttttgttcc cgaaaaaagg gaacccaccc agggacggaa cgtaggagca atcgatgagg
     1141 atccgcatca aacaggtcag cctcgtgctg atcggcgtgc tggcaggcgt gctgctcagc
     1201 ttgaacttct cggcagtcgc gcaacgcgac gggacgcgtt acccgctgcc ggtggacgag
     1261 ttgcgcgcct tcgccgaagt gttcggcgcg atcaagtcca gctatgtcga gcctgttgag
     1321 gacaagaagc tcatcaccga ggcgatcagc gggatgctga ccggcctcga tccccattcg
     1381 gcctatctcg accaggacgc cttcaaggag ctgcaggtcg gcacgcaggg tgagttcggc
     1441 gggctcggca tcgaggtcgg catggaagac ggtttcgtca aagtcgtgtc ccctatcgag
     1501 gatacgcccg cgttccgcgc gggactgaag cccggcgacc tgatcatcaa gctcgacgac
     1561 acgcccgtga aggggatgaa tctcaacgac gcggtcaagc gcatgcgcgg caagccgaag
     1621 acggccatcc gtctcaccat catgcgcaag gggggtgact cgaagccgtt cgaggtcacc
     1681 ctgatgcgcg acatcatccg cgtacagagc gtgaagtcga agctcatcga gcccggctac
     1741 ggctggatcc gcgtctctca attccaggag cacaccggcg agaacctggt gaaggcgctc
     1801 gagggcctgt acaagcaggg cccgctgaag ggcctcgtgc tcgacctccg caacgacccc
     1861 ggcggtctgt tgaacggcgc cgtcggcatt gccagcgcct tcctgcccgc gcgcgcgctc
     1921 atcgtctcga ccgacggccg caccgaggac gcgaagcgca agttcctggc gtcgcccgag
     1981 gattatctgc gcggcaaccg cgaggactac ctgaaggccc tgccggcggg cgtcaagacc
     2041 gtcccgatgg tcgtgctcgt caacggcggc tcggcttccg cctcggagat cgtcgccggt
     2101 gcgctgcagg accataagcg cgcgaccgtc ctcggcacgc agacgttcgg caagggctcg
     2161 gttcagacga tcctgccgct cggcaacaac actgcgatca agctcacgac cgcgcgctat
     2221 tacacgccga gcggacgttc gatccaggcg aagggcatcg agcccgattt cgtgatcgag
     2281 gatccaacct cgcccaccac cgggcggctg cgcgaggtcg atctcgacaa gcacctgctc
     2341 aacgagcagg acaagagtat cgagaagctg caccgcgacg aggccgcccg cgccgccggc
     2401 aagaccggca cggacgacaa caagcccgca gcgcctctcg agttcgcctc ggacaaggac
     2461 ttccagttcc agcaggcgct gaagttcctg aagggccagc cgctcaccac cgcgcagagc
     2521 gccgccgcga cggcggtgcc cgtggtcaag aagaactgac cgcgatgagg cgccgcaagg
     2581 cggcgcctca ttcacctccg tgaccgacga gcagctgctg cgatattcgc ggcacatcct
     2641 gctcgccgaa ttcggggtcg aggcgcagga gaagctgatc tcggcctcgg tgctcgtcat
     2701 cggcgccggg gggctgggct cgccggccgc gctttatctc gcggccgcgg gcgtcggaac
     2761 gctcacgctc gccgacggcg atgccgtcga cctcaccaac ctgcagcgcc agatcctgca
     2821 tacgacgcgt tcggtgggaa cgccgaaggc cgagtcgggc aaggcggctc tcgcgcagat
     2881 caaccccgag gtccgcgtcg ttccggtgtc gcgccggctg gcgggcgacg aactcgagcg
     2941 cctggtcgcc gcgtccgacg cggtgctgga ttgctccgac aacttcgaga cccgccacgc
     3001 ggtcaaccgc gcatgcgtcc gccacggcag gccgctggtt tccggggccg ctgtgcgctt
     3061 cgacgggcag gtgacggtgt tcgatctgcg ggacgaggcg agcccctgct acagctgcct
     3121 gtttcccgaa gacgccgagc tcgaggagac gcgctgcgcg gtgatgggcg tgttcgcgcc
     3181 gctcacgggc atcgtcggga cgatccaggc ggccgaggcg ctgaagctca tcacgggcgt
     3241 cggcacgacg ctcgccgggc gcctgctgat catcgacgcg ctttccatgg agtggcgcag
     3301 cgtgaagctc gcgcgcgatc ccggctgccc cgtgtgcgct acacgagcag cggccactgc
     3361 ttatcccagc cctccgtcgg caaccgcctg aagccgctct tcgcgaactg ctgccagcgg
     3421 ccgatcacgt agcaaagcag cagattcgcg tgcgcgcccg gttcggcctg tgccgcgccc
     3481 gcctcggtcc ccgccacgcg caggcactgc ttcagcgtcg cctcgaggcg atcgtgaagc
     3541 tggttgatgc gggcctgcag ccgttcatcc tcgttgacca gggcgtcgcc gatcagcacg
     3601 cgggtcatgc cgggattctt ctcggcgaag ccaagcagcg acgcgaggat cgcgtgcgcc
     3661 tgcttgatgc cgtcgtcctc ctccgccgtg atcttgttga ccagaccgaa cagcgactgc
     3721 tcgatgaact cgatcagccc ctcgaacatc tgcgccttgc tcgcgaagtg ccgataaagc
     3781 gcagcttcgg aaacgtcgag cttctccgcg agcgccgccg tcgtgatctt ctcccctttg
     3841 ggatcctgca gcatctgcgc cagcaccttc aggatctgca agcgccgttc gccgggcttg
     3901 gcgggcatgg ggcctccggg acggtcgggg gagcgacatt gtatgactaa gggtaagcgc
     3961 tcgctagctg agttttgaca ggtcatcggc gagccgcggc agcgcgagca ctgaggcgac
     4021 cctcacgtcg accgagaggg ggacgcggac ctcgcgcgag acccagacgg tcttcattcc
     4081 cagggtcttg gcggtgcgga gattctcgac ggtgtcgtcg accagcacac agcgctcggc
     4141 aacgaggcgc tcggcgcgga tcatgacgcg gaagccgcgg cgtgagggct tcgggtggaa
     4201 gcgcgcgcgc tcgatgcaat agagcgcgtc gaacgacgtc tccaggcgta gcgcgcgcag
     4261 gatgcgctcg gcataggcga ggggcgcgtt cgagaacagg agcttgcgtc ccgggatgcg
     4321 ccgcaacgca tggcgcaagc cgcgctcggc tagcaccagg cgctcgaggt ccgggaggtg
     4381 gtgcgtctcg tgcagaaacc ggcgcggatc ggtgccgtgg aagcgcatca tgcccacgag
     4441 cgtcgcgccg taccgcagcc agtacgcgcg ccgcaaccga tcggcctccg cctcctcgag
     4501 cccgaggtcg cgccgcagga actcggtcat gctgcggttg atgtgcggca tgatgcgcga
     4561 gcggtagtcg tgcagcgtgt tgtcgagatc gaaaatccag gtcaggcgat tcgctgccat
     4621 ccagggcccg tcttcagtaa aatttttcca tggccaggct ctcaccccgc ttcagcctga
     4681 agcacctcat cgtctcggag acggcggccc ggcgcggcct ggacaaccga ccgccggcgc
     4741 gcgtggtacg gcggctgcgc aggctggccg ccggccttga gcgcgtccgc gcgatcacgg
     4801 ggcgcgatct cgacgtcacc agcgcctatc gcggcccggc gctcaatgcg ctcgtcggcg
     4861 gcagcagggc ctcgcatcac atgcaggggc tcgccgccga tttcagctgc ccgcgtttcg
     4921 gctcgccgtt ccgcatctgc caggcgatcg tgcgcagccc attgcggttc gaccagctca
     4981 tctacgagca cggcgatgcg gacgaccgcg gctgggtcca tctcagcttc gcgccgaaaa
     5041 tgcgccggag agtgctgacc atctgcaagt cgcggggcac gtaccagcgc gggctgcgcc
     5101 gctgtcccgt cgatctactt tgagcggatg agggtgccga caccctcgtc ggtgaggatc
     5161 tcgagcagca gcgcgtgctc gacgcgcccg tcgatgatgt gcacgctcgg cacaccgctg
     5221 cgcgcggcgt cgagcgccga gctgatcttc ggcagcatgc cgccggacag cgttccgtca
     5281 gtaaccaggg cgtcgatctt cttgggcgaa aggccggtga ggagcttgcc gctcttgtcg
     5341 aggacgcccg gggtgttggt gagcacgatc agcttctcgg cacgcagcac ctcggcgagc
     5401 ttgcccgcca ccaggtcggc gttgatgttg taggactgac cgtcgtcgcc gacgccgatc
     5461 ggcgcgacga ccgggatgaa tcccgcctgc tcgagcgggg agatcacgct cggatcgatt
     5521 gatgcgatct ccccgacctg gccgatatcg atgcggccgt tgcttttgtc cgagagcagc
     5581 atcttgcgcg cccgtatgaa ggcaccgtcc tgcccggtga ggcccacggc gcgcccgccc
     5641 gcctggttga tcagcgtgac gacctccttg ttcacctggc cgccgagcac catctcgacg
     5701 atgtccatgg tctcggcgtc ggtcacgcgc atgccctgca cgaattcgcc cttcttgccg
     5761 acccgtgcga gcagctcgtc gatctgcggc ccgccgccgt gcaccaccac ggggttcatg
     5821 ccgacgagct tcagtagaac cacatcgtgc gcgaagctct ccttgaggcg ggcgtcggtc
     5881 atcgcgttgc cgccgtactt cacgacgatg gtgcggccgt ggaaacgccg gatgtagggt
     5941 agcgcctcgg ccagcacctt ggccttctgc gcaggcgtga gggcctcgct cgtgatggcc
     6001 ccggtcacaa cgcctttcgt cgcgctcgtc atcgaacgcc gattgtaccc gcgcgggccc
     6061 caaaaaagaa aaggcggccg gggccgcctt tccgggccgt gctgcggcgc cgtttattgg
     6121 atcgtgagct gcttcgcggc cgccgcggtc ttcttcggca gcgtcaactc gagcacgccg
     6181 tcgatatagc gcgcactggc cttcgactcg tcgacttcct gcgccagccg gaacacgcgc
     6241 gacaccttgc ccacctgccg ctcggagtgg atgacgcgct cgccatcctt cacgtcgcgc
     6301 tcggcgcgcg tctcggcggt gatcgagacg tggtcgccgt cgatgttgac ctggatgtct
     6361 tccttcttca ccccgggcag ctcggccagc accttgtact cgccgttggc ctcggtcacc
     6421 tcgagcttca cgcgacccac cggctcgccg gcttcgaacc cgacgggacg cacgaagaag
     6481 cccttgaaga aatcatccac cagatccccg aagggatcgt aacgggtaat gcttgccatg
     6541 atcgctctcc tatcaatatc ggccgggcct gcagcccgta tgctgggaaa aatggggaga
     6601 gcatgggaga attcaaggcc ccacgagaca ccgataaatc atgccgcacg ctcttgaaaa
     6661 ggccgccccc ggccccactc ccggcgagat gcgctgcgcc gcgtgcggag aggttttcgt
     6721 ctgcggcggc cgggcgggtt ccgcgtcgtg ctggtgcgcg gagctgccgc cgcggctccc
     6781 ggtgcccgat gcctcggcgg cctgctattg caggtcgtgt ctcgaacgat tgatcgcgag
     6841 ccgctagcgg cgcgcgccgg atgcgaaatc gcgcacttcc gcgctgacat cgacgcggcg
     6901 gcgttttccg ccggcggtct cgacgaccag acgcagcggg acccggtccc ccttggcgag
     6961 ggggcgcttc agtccggtga gcatcacgtg cgcgcccgag gggtcgaggg ccaccacgcg
     7021 gccggccgga agctccagac gcttcagcgg gcgcatccgc atgatgtctc catccatctt
     7081 catctcgtgc aactcggccg tgccgatcgg gctttccacg gcgacgatcg ccgccggctc
     7141 gtcgctgcgc aaccgcatgt agaccacgct cgagctctgc cccgggaccg tgccgcgcgc
     7201 ccatgcatcg gtcgcgtgca cctgcgcggc cgctggacgc gcgagcgcgc acaagagcga
     7261 cagcccgagg gcgcgcgcga ggaccacggc gcccctcaca gcacgtagcg cgcgagatcc
     7321 tcgctccggg cgaggtcctg cagccgcgca tccacatagg cggtgtcgac gacgacggac
     7381 tccttgccgc gcttgccggc gtcgaacgaa atctcctcga gcagcttctc catcacggtg
     7441 taaaggcgcc gcgccccgat gttctcggtc ttctcgttga ccgcataggc gatctccgcg
     7501 agccgccgta tgccctccgg cgtgaacgac acctcgaccc cttcggtcgc gagcagcgcc
     7561 tggtactggc gcgtgaggca ggcatcggtg ttggtgagga tctgctcgaa gtccgacacc
     7621 gaaagcgagt cgagctcgac ccggatcggg aagcgcccct gcagctccgg gatgaggtcg
     7681 gacggcttgg agagatggaa cgccccgctt gcgatgaaga ggatgtgatc ggtgttcacc
     7741 atgccgtatt tcgtggcgac ggtcgtcccc tcgaccagag gcagcaggtc gcgctgcacg
     7801 ccctggcgag acacgtcggc gcctgccatc tcaccgcggc tcgcgatctt gtcgatctca
     7861 tccaggaaca cgatgccgtt ctgctcgaca ttggcgagcg ccttcgcgcg caggtcctcg
     7921 tcgttcacga gccgcgcggc ctcctcgtcg gtgaggatct tcatcgcctc gcggatcttc
     7981 atcttgcgcg ggcgcttcct cgcgccgccg aggttctgaa acatgccctg gatctgctgc
     8041 gtgagctcct ccatcccggg gggcgcgagg atctccatat gggcctgcgg agctgcgatc
     8101 tcgatctcga cctcccgatc gtcgagctcg ccctcgcgca ggcgcttgcg gaatttctgc
     8161 cgcgttgccg attcctcctc gcccgtcctc ggcgcggcgt cgacctcggc cgcgaacgcg
     8221 tcgagccggc gcgcgccggg cagcagcaca tcgagcaacc gctcctcggc agcgtcctcg
     8281 gcgcggcggc gtactttgcg cgtcgccgcc tcgcgcgcgt ccttgatcgc gatctcgacc
     8341 aggtcccgga tgatggtgtc gacgtcgcgc ccgacatagc cgacctcggt gaacttggtc
     8401 gcctccactt tcacgaacgg cgcgtccgcg agccgcgcga gccgccgcgc gatctcggtc
     8461 ttacccactc cggtcgggcc gatcatgagg atgttcttgg gcgtgatctc ctggcgcagc
     8521 ggctcgccca cctgctgccg tcgccaccgg ttgcgcagcg cgatcgcgac cgagcgcttg
     8581 gcgcgatgct ggccaatgat gtgcttgtcc agctcgtgga cgatttcctg gggggtcatg
     8641 gaagacatga ttgcgaaccg gagattggga cgcgcgccgt ggcgccgccc tcattcgagc
     8701 acttccacca ccgtgttctg gttggtgtag atgcagatgt ccgctgcgat ggccaacgcc
     8761 ttgcgcacga tctgctcagc cgagagcgtc gtattgtcga gcagcgcgcg cgccgcggat
     8821 tgtgcgtaag gaccgcccga gccgagcgag atcatgccca gctcgggctc gagcacgtcg
     8881 cccatgccgg tgatcaccag cgaggactcg cggtccgcga cgccgagcat cgcctccagg
     8941 cggcgcaaca tgcgatcggt gcgccagtcc ttcgcgagct cgaccgcgga gcgcagcagg
     9001 ttgccctggt gcttctcgag cttggcctcg aagcgctcga acagcgtgaa cgcgtccgcg
     9061 gtaccgcccg cgaacccggc aaggatgcgg tcgtgataca gccggcgcac cttgcgcgcg
     9121 cccgacttga cgacgacgtt ccccagcgtc acctggccgt cgccgccgag cgcgaccttg
     9181 ccgtcgcggc ggacgctgag gatggtggtg ccgtggaatt gttgcatgga tggccgggtg
     9241 ggcggccggg aagccgagcc gcgagcgtca gttcgggacg aaaacccgga tttcaagccc
     9301 gctcatttgc gtctgctcat ttgcgcctga tgacggtggc gaccttcgac acgcccatga
     9361 cttcgaacag cgccgcgatc atttcgttcg cgccgcggat ttgtatcgtc gtcccggcct
     9421 ggcgcagctg cgagagcgcg ttcagcatca cccccgcgct gacgaagtcg acgcgcctcg
     9481 ccttcgacat gtcgacgacc agcatgctgt tcgcggcggc ccagtcctgc agatccttga
     9541 ccagcgcctc gccggccgaa gtcacgtcgc ccgcgagcgt gagtgccgtg gccggcgaat
     9601 cgcccacgct tcgcgccacc ggagccttcg tcgccgcggg cggccgctgc tcccacgacg
     9661 gcggcgagac ctcgtaggtc acggcgtagt tcaccgccgc ttcctcgaag gcctcctggc
     9721 gcccgaacca cttgtacacc tcgaacagca gcgcccacat cgctcggtcc gtgcccgcgt
     9781 tccctgcgct gcacgcgccc tcgagcagct cgagcagccg cgcctcgccg gtgaggacga
     9841 cttcctttcc gcccgagcgc agatcgacca gcgccgctcg caggcgctcc gcggccgcag
     9901 tctcgatcgc ctccatcctg ccgaagtcca cccggatcat ctgctggcgc tgcgccaggt
     9961 tggcgagctt ttcgaactct cccgcgccgg cggcaatcga gcccgcgaag gcgcagtagc
    10021 ccgttccacc cgtcagcagc gccgggttcg cacgcgcctc gacgccgccg tcgcgccacg
    10081 cgggcgccga gcgctcgaac ttgaccgcga actcgagcgc gagcgattcg tagtcctcgc
    10141 ggcgcccgag atgctgatag acgtcgaaca gcatgagcca gcactgcacg gccgaagcgc
    10201 ccaggtcttc accccgcacc gcctgcgaca gcttcgcgag cgcctgctcg gcctggccat
    10261 tggcgaacag gatcgccgtc tcctcgatca ccggcgcgac gtcgcccgag gccgccgtga
    10321 cctcgatgct catcaccgag tccggctcgc gcggcgcgcg cgcggggacg gccgcctgcg
    10381 gcgccgatgc tcgtgcggcg gcggtcgcga gctcgcgctt gggcaccggc gtatagccgg
    10441 tgaaatcgag cgaagtcgac ggttcgtcga gtgcgacggg gcccgcaggc tgcgacgccc
    10501 gcgaaaggga tctcgccggc tgcggcttgg gcgccggcgg cttggcggcc ggctttttcg
    10561 ccggctgcgg agccttcttg ccgaatagcg aaaacacctg ggaagacctc ggttctacgg
    10621 ggtactgcgg ggggtacgga gacttttatc acaggagcgc ggccgagcgc aatgtacggc
    10681 gatctttgcg catccggagc gcctcaggcc gcggagcgcc tgcggcccag cgccgccacg
    10741 accgcgccga tcgcgacgat cgcgcacacg atcattgccc ccgagggcag gtccgcgacc
    10801 gccgagacca ccaggccgac cgcgtacccg agcacgccga cggcatacgc cttcaggtaa
    10861 cgcttccggg gatcgtaata ggtcgagagc gcgggcacca cgagggtcgc gaacaccagg
    10921 tacagccccg cgagctgcac cgacatcgtg accgcgaccg cgaacaccgc gtagaagccg
    10981 atgcgcccga gacgctcccg gagcgtgaac cacacgccga ggatgccggc cgagacgagc
    11041 gcgatgagcg gcagcatcga gggattcacc cagaggatct ggccgatgag caactcgcgc
    11101 aggttttcgc tccccctcgg gttcttcgcg aggagcagca gcgcgacgct cgagcccacg
    11161 acgaagacca cgccgatgat cgcttcctgg acctcgggcc agcgcttctc ggtccacgtg
    11221 agcagcatcg cgcacgcgag cgccgcgccg agcgcggcga tctgaacggc gctaccggtc
    11281 ggctcgaagc cgaggaagtc ggcgaggatc acaccgaggc cggcgacctg cgccaccgcc
    11341 aggtcgatga agacgattcc gcgctggagc acttgcatgc cgagcggcac atgcgtcgcc
    11401 gtgaccagga gcccggcgac gaacgccggc cagagaatcc cgaagctcgc ggcgtcgagg
    11461 ttcatttgat caccgcgagc agacgcgcga gcgtgtcgtc gaacaggccg aacaggtcct
    11521 ttgccccgtc ggtaccgccg acggtgaacg gcatcatcgt cgtcggtatg ccggcgcgtt
    11581 gcgacaggaa ctcggccgcg cgcggatcgt tgtacggaga gtacaccacg accttcgcgg
    11641 gatcgcgctt catctgctcg accagctcgg cgagatgact cgggctcggc gggatcccgg
    11701 gcttgggctc gaggctgccc acctcgcgca tgccggccca gttgatgaag taggagaagt
    11761 cgcggtgata gacgacgatg gcgacgccct tcaggcgctc cttgtcgcga tcccagcgct
    11821 gcatggccga cgcccagcgc cgtccgaagt cggccgcgcg gtcgcgatag aaggcggcgt
    11881 tgcccgcgtc gacgcgcgcc atccgctcgg cgagcacctg cgcgaccttc gcgatgttgc
    11941 gcggatcgag atggacgtgc gggtttcccg aaggatggat gtcgccgaga gagcggtcga
    12001 gaaccttcgg cacctcgagc ttgggcacga actgagaggc ttcgacatag cccggcgagc
    12061 ccgactggat cctgggattt cccgactgcg tcagcagcag cggcagccag ccgacctcga
    12121 gctcggcgcc ggtgcacacg aggaggtcgg cccgtcggat gcgggcgatc aggctcggcc
    12181 gcgcctcgag gcggtgcggg tcctggcgcg cggtcgtcgc cgaggtgacg gacaccttgt
    12241 cgcctccgat ctcctgcgcg agcgacgccc attcgggctc gcaggtgaac acgttgagcg
    12301 ccgcggacgc ggaccacgga agcgccagcg acaacaggaa caaggcggtc ttgagcagga
    12361 tcttcatgtt cgttttctca gaacgtgtgg gcgccgtgcg cgccgaggct gacgatgtat
    12421 tgaaggaaaa cctggttgtc gggctcgttc tgtcgcgagc ggtcgcgcgc gagctgcagc
    12481 cgcacgcggc tgaactcgct cgcgctccag tcggccatca ccgtgttgcg cgtcgggttg
    12541 tagtgtccga ggacggggaa gtcggcgggc gtgagcgcgc ccgaatccac caggccgatc
    12601 gtctgcgtgc cggcgctcag ccgatcgtag cggtagccca cgcgccacat gggcaggaat
    12661 tgatacaccg tctgcagata ccacccggac tggcgcgacg cgaacgcgcc ggtctgcggg
    12721 ccggccgccg ccgcggccgt gtcgaacgtc agatcgccgt cctctttgcg gcggaagtac
    12781 tcgccctgca gcttcacgct cgtctcggtc gagttgaagt ccggcgccca tttcaggacg
    12841 ccgctcaagg cccacagcct gctgcggccg ctgaaggcgt tcgtcaccgc gatgcccgcc
    12901 gaatcggcgt cctggaacgt gcgatcgcgg ggcgaggtgc gcagatacga gagccccgcc
    12961 tgccaggcga agcccgagcc gaaatcgccg ccgaggtggc cgaacgcatt cgccgagccg
    13021 acgccgttct tgtcgcgcgc tgcgccttcc ggcgcggcgg cggggaaggc gcggccgcgg
    13081 ccgagctcga ggccgagatc gaaataggtg tcggtcgggg cgacccattt cagctgcacg
    13141 ccgtcgtcgg cgagctggtt gccgagaaac accttgttgg cgagcggcgc gtcggtgaaa
    13201 tcccaggcgt gggcatggat ctggttctgg tacccgaccc ccgagaagaa gcggccggct
    13261 ttgaccgtga agccatgcga gagcgcgagc gtctggatgt acgcctcttc ggcggcgacc
    13321 tcgttctgcg gcgtgatgga gaagatcccg gtcccgcgga agtccggatc gatattggcg
    13381 gcgagcgcca gctcggattc gccgaggctc gggcccctgg ggaagggctc gacttcgccc
    13441 agcgtcggaa cgaagccgtt gatgcggaaa tcgcccggac tgcgcgacag gctcgagtag
    13501 acgccgttca ggatcagcga cacggccggg ttgagcgcgt tctcgcccgc agggcggttc
    13561 ccggcctgcg cggcctggtt cgcggcgctc tcggcgtcct gcacgcgctt ctccagcgcg
    13621 tcggtccgct ccttgagctg cctcacctcg tcgcgaagcg ggtcgttcgt ttgagcgaac
    13681 cccgggcctg cgatgcccac ggcggccgcg acggcaagag ccagtgtgct ggtcttctgc
    13741 atgttcttct ccgaaatcac gcgatcgtcc gctggagacg acccgatccc cgcgcgcgga
    13801 ggcgcgggaa ggatgacgcg ggactaggaa aagaccggcg gcgctcgcga atcgaattcg
    13861 atccgcgact ggaacggaag cggaagaacg aaatacgaga cgcccggcgg cgcgacgcgc
    13921 gccggcttca ccgcgcgcac cgcgttcggc aggacgctgt cgaccgcgtg gaaagcgaga
    13981 cactgcgcgg cgggatgccc ggagaagggc atgcccttct tcgccggatc gtgcggaacg
    14041 tgggagagcg cgtgcagctg tgcagactgc tgcgcgaaga tcagcgcagc ggcgatcagc
    14101 acgtaccgca aagtgcgact catggaaccg cccgggatat cggggcggct agtgtagcgc
    14161 aaatcggcgc gatggcgccc gacgcgccgc cgtctcgtta ttcgccgtag agcttctgct
    14221 tcagctcgcg gcgcgcctgc gcctcgatcg tgagcgtcgc ggtcggccgc gcgagcaggc
    14281 gcgggatgcc gatcgcctcg ccggtctcct cgcaatagcc gtagctgccg tcctcgatgc
    14341 ggcgcagtgc ctgctcgatc ttcttgagca gcttgcgctc gcggtcgcgc gtgcgcagct
    14401 cgagcgcgta ttcctcttcg atggtggcct ggtcggtggg gtcgggctcg agctcgtgct
    14461 cgcgcaggcg ctctgaggtg acgtcggcgt tctcgaggag ctcgcgctgc atctcgagca
    14521 gccgctcgcg gaagaacgcg agctgctgcg ggttcatgta ctgcgccgcg ggcgccttgc
    14581 ggatttcgac ctcggtgagc ccgctcgcct tttcctccgt cgggcgtgcg gcagccggct
    14641 tggccgcccg tgcgtccttg ggcggcgtcc gtttcactgc gttgcttcca ggcattgttc
    14701 caaccccttg ataaagagat cctgcggaag gttcttgccg atgaagacca gcgtgctcga
    14761 acgtttctcg tccctgcccc acggccgccc gacgtccccg cccatcagca tgtgaacgcc
    14821 ctggaacact acgcggttcg tgttgccttt tagatacagt acgcccttgt agcgtagcat
    14881 gttcggaccg tagacctgta tcaatccgga cagaaattcc tccagcctga cgccgtcgaa
    14941 gggcttgtcg gagcggaaca cgaacgagct cacctgctcg tcgtgctcgt gctcgtcgtc
    15001 ctcgaggaac tcgggatcga gctcgagaat ggcattcagg ttgaagccgc ggatatccag
    15061 gatctcgtcg atcggcgcgt tgccgaaatg cacgttcttg atcggcgcgc gcgggttcat
    15121 ctgcaccagc cgcttgcgca ggcgcgattg ctcgtcctcg ggcacgagat cggtcttcga
    15181 cagcaggatg cggtcggcga aaccgacctg ttcctgcgcc tcgtggaatt cgtcgagctg
    15241 cttcggaccg tgcttcgcat cgacgaccgt gatgacgccg tcgagcagat agtagttgcc
    15301 gacgtgctcg tcgacgaaga aggtctgcgc gaccgggccg ggatcggcca tgccggtcgt
    15361 ctcgatgatc acgcgctcga acttgagctt cccgctctcg cgccggtcct tcagctcgcc
    15421 caggatgcgg atcaggtcgc cgcggacggt gcagcacacg cagccgttgt tcatctcgac
    15481 gatctgctcg ccttcgttct cgagcaggat ctcgttgtcc acgccggcct cgccgaactc
    15541 gttttcgatg acggcgatgc gatggccgtg ctgctccttc aggatgcggt tgatgagcgt
    15601 ggtcttcccg ctcccgagaa accccgtgag gatggtgacc ggcaccatgt tgtcttcagt
    15661 ggccatgacc cattctatgg agattccgcg ccgcgtgtga cctgcaaggg cttgcgcgag
    15721 ttcaccgcgg accgcggcag ccgctccggc gtcgcgtcga gccggaagcc ggggcggccc
    15781 ggaacggaag ccccgaaaaa agcgcgcatt agaccacggc gcctcgtgcg acgcaacccg
    15841 cgccggtcac acccgctcga gcagcaggcc cttcaggtac tcgccctccg ggaactccac
    15901 gagaaccggg tggtcgggcg cggcccagcg ccgctcgcgc agcaggagcg aagcgggcgc
    15961 atcggctgcc gcgcccgcca cgatcttctg gaacagctcc ggggacacgc cgcccgagca
    16021 cgagaacgtc gcgagcaggc cccccggccg cagcagccgc agcgcgttca ggttgatgtc
    16081 cttgtatccg cgcgccgctc gctcggcgtc cttggacgtg ggagcgaact tcggcggatc
    16141 gagcacgatg aggtcgaacc ggcgctgctg ggcattcagc tcgcgcagca tccggaacac
    16201 gtcgccctgc agccattgcg cggcggcggc caggccgttg cgctcggcgt tcgcctgcgc
    16261 cgcgcgcagc accgcggccg agctgtcgac cgacagcacc gagcgcgctc cgcccgcgag
    16321 cgcggacagg gtgaatccgc cggtatagca gaagcagttc agcacttcgc ggtccctgga
    16381 cagctccccg acgcgcgccc ggttgtcgcg ctggtccaga tagaagcccg tcttctgtcc
    16441 gtgggcgacg tcgaccgcgt agcgcaggcc gtgctcgcgg atctcgacca ccggctcgcc
    16501 ggccgcgccg ctcgcccatc cgtcgcgcgc tgcaagcccc tcgagctggc gcgcctccgc
    16561 gtccgagcgc tcgtacacgc gctcgcatcc cgagagctcg gcgagctgcg cgacgagggt
    16621 cgagcgccag cgctcggctc cggcggaaag cagctgcacg accagcgtgg cggcgtatcg
    16681 gtccgcgatc acgccgggca ggccgtcgga ctcggcgtgc acgaggcgca gagcgtcggt
    16741 gtctacgttg atgcgggccc cgatgcgcgc gcggcgcaac gcgagcgccg ccccgatctt
    16801 ttcccttatg agatcgggac ccggaggccg cggctcctcg aagctccaca cgcgggcgcg
    16861 gatctgcgac gaggggttga acgctcccca ggcgaggaag cgtccgtcgt tcgtgacgat
    16921 cttcaccgtc tcgcctgcgt ccccggtatt ttcggcgcgc tcgaccgcgc ccgagaacac
    16981 ccagggatgg cggcgcagca gcgacttctc gcgtcccggc ttgaggacga gcttcccggc
    17041 ggcgcgctca gttctttctc ttggcgcgcg ggtgcgcggc atcgtacgcc tttgccaggt
    17101 gctggaagtc gaggtgcgtg tagacctgcg tggtcgagat gctcgagtga ccgaggaggt
    17161 cctggacggc gcgcagatcg ccggacgatt gcagcaggtg cgacgcgaac gagtgccgca
    17221 gcacgtgcgg atggacgggc gcggcgatgc cgagcttctt cgcccagcgc gcgagacgat
    17281 gctgcacgac gcgcggcgcg atgcggcgcc cgcgcgcgcc cgtgaacagc gcgcgctcgc
    17341 ctgcacgcgc gagtgccggg cggatcgaga gccaagcgcg cagcgcctcg atcgccttgc
    17401 ggccgaccgg cacgcggcgc gtcttgttgc ccttgccgag caccgcgagc tcggcctctt
    17461 cgatcgccgt gcgcgcgtcc tcgagatcaa ggcgcaccag ctcggcgagc cgcaggcccg
    17521 aggaatagaa cagctcgaac atcgccttgt cgcgcgtctc ccggtcgtcg tcgggccggc
    17581 gatcgagcag ctgcgccgcc tggtccgggg agaggacgcg cggcagggcc ttcgcgcttt
    17641 tcggggcgcg gatgccggcg cagggattcg ccccgagacc gcgatggcgc gcgagccagg
    17701 cgaagaacga gcgccatgcg gacagcgccc gcgcgaggct cttccccgag aggccccgcg
    17761 cgtgcaggcg ggccacggcg cgtcgcacga gatgcgacga gacctggtcg agcggggcat
    17821 cgtcgatttc ctcgagcagc cgctcgatat cgcgcgcgta gcccgagcgc gtgtggggcg
    17881 acagccggcg accggattcg agcgagcgaa gaaacgccgc gagcagctcg cgcttcgcgt
    17941 caccgtcggg cgcgggcatc cgcgccaccc ctaaagaaag cgcagcaggg acgcgctcgc
    18001 gagctcgccg agccgcttga gatagaccgt gcccatctcg gggtagaaac gcgcggcatc
    18061 ctcgctgccg aggaccagca tgccgaagca ctcgcctttc tcgcgaagcg ggacgctcgc
    18121 caccgagcgc acatgcgcgg ccgcctcgcc gaaccaggcg acgacctcgg aattcccggc
    18181 cggcccgcag aaaggctggg cgagcccggc cgcgtaatgc ttcgcgtcat cgctcgcgcc
    18241 gccgagctcc gcgagctcgg ccgcggctcc ctcgcgccag acgcgcaggg cgacgtgcgg
    18301 aacggcgaaa tcctcgcgca ggttgtagtc gagcgccgac agcagggcgc gcaggtccgg
    18361 cacgggcagc agcgccaggc agagccggtg catcttctcg ccgatcgcgt cgttttcctc
    18421 gccgacctcg agcagctccg ccaggcgcga ctcgagcgcg cgggcccgct cgcgcaaggt
    18481 cagcacttgc cgctccgaga gcggtatggc gcggccgccg tgcggatgcg ggatgtagat
    18541 ctgggacaac aggtcggcgt actgctcgaa gaaaaccggg ttttcctgca ggtagcgcgc
    18601 gacgtcgtca ggtctcaacg ttgatttccc cttcgaacac ggtcacggcc ggtccgctca
    18661 tccatacggg attgtccccg ccccgccact cgatcgtcaa atcgccgccg cgcgtcgcga
    18721 cgcgcaccct ggcgtcgagc agcccgcgcc ggatgccggc cgcgacggcc gcgcaggcgc
    18781 ccgtgccgca ggcgagcgtc tcgcctgcgc ctcgctccca gacgcgcagc gcgatgcggc
    18841 cgcggccgag gacctgcatg tagccggcgt tcacccgttc ggggaagcgc ggatgcttct
    18901 cgatgacagg accttcggtg gtcacggggg cagaatcgac gtcgtcgacg agctggaccg
    18961 cgtgcgggtt gcccatcgag accgccgtga tctgcaccgt tcgccccgtc ggcagctcga
    19021 gcggctgcac cggggccggc gattgcgaca ggaacggaat cgacgcggga tcgaagcgcg
    19081 gcgagcccat gtctacggtg acctcgccgt cggcctccag ccgcggaacg atcatgcccg
    19141 acgcggtctc gaccttgagc tcgcgcttgt ccgagagcgc gtggtcgcgc acgaagcgca
    19201 cgaagcagcg cgcgccgttg ccgcactgcc cgacctcgcc accgtcggcg ttccatatcc
    19261 gatagcggaa gtccgcgccg gccgaacccg gcttctcgac gagaagcacc tggtcgcagc
    19321 cgatgccgaa gtgccggtcg gcgaggcgcc gggcgagcgc cggcgtcatc gcgacgcgct
    19381 ggcgcacgcc gtcgatgacg acgaagtcgt tgccgagccc ctgcatcttc gcgaacttca
    19441 gcttcacggc cggtattcta agcgtccgct cagtaccacc gcggctcgcc cggcggacgc
    19501 gtcttgaagc gcttgtgcgt ccagaagtac tgctcgggca tctcgagaac gcgctcctcg
    19561 atgaaggcgt tcatgcgccg cgcgtcggcc tccgggtcgg cgccgggaaa gtcctcccac
    19621 gccggatagc acctgacctc gtagcccgcg cccccgggca gcatgcgcgt gacgcacggg
    19681 atcacgcgcg caccgaccag ccgggcgagc cgcgacaggc cggtcacggt cgccgccggc
    19741 acgccgaaga acgggacgaa gatgctctcg cgcgcgccgt aatcctggtc gggcagatag
    19801 tagaacggga cgtggtcctt cagcaccgcg atggcggccc gcacgccctc ctggcgcgag
    19861 atcgcacgct ggtggccgaa gcgcgtccgc ccgtgcagga gcatcgcatc gaagacggca
    19921 tccttctgct tcgcgtagat gcccgccatg tcgatctcgc aggtgaggcg cgtgcacccg
    19981 gcgtccaggc ccacgaaatg cggcgcgagc agcagcacgg gcgcgtcctt cgccgcgtgg
    20041 atgtgctcca tgccgacgat gcgcacgagg cgcacgatgc gctcgcgcgg cgcccaccac
    20101 aggatgccgc gctcgagcac gctgcgaacg aaggcaccga aatgccgcct cgcaatgcgg
    20161 acgcgctcgc cgggcggcag gcccggaaag cagcgctcga g
//
LOCUS       DATDMW010000299         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_339221, whole genome shotgun sequence.
ACCESSION   DATDMW010000299 DATDMW010000000
VERSION     DATDMW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339221"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VI319_12610"
     CDS             complement(<1..1123)
                     /locus_tag="VI319_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864730.1"
                     /translation="MRWVWRTLAVVAVLLLLIVGAITWVMTTPEGARLVLGRVSTMLG
                     EGTTFSGVDGRIGGLLRVDTIEISRPDMYVRVEGFEMDTAPLEPLHGRLLVHRLVARS
                     VVVRTASTGEAAKIPVSFKPPYAVDLEDGRVGELRIGPLTPEARAEKDVARRRALMEA
                     TRDRDFVVRDIVLRGEGDASRWTIHEARAETTYGRAALAGTLETTAPFALEAKARGEG
                     SFGERPYAVELHAAGTLKRLEATFEGDVSGQHAVGRAAVEPFAKMPLRTLEATAKELD
                     LARIAGSLHTNVDVQAKLDSQGDAFSGPVRIENHAPGPWDKGRLPFNAASARVVLTPE
                     RVDVADLSVALPGGGAASGQAVVQKSGIEANLRVADVDLL"
     gene            complement(1185..>2259)
                     /locus_tag="VI319_12615"
     CDS             complement(1185..>2259)
                     /locus_tag="VI319_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY6864731.1"
                     /translation="DTLGELQRRLQSGPWFSTVTVDVERDPLKPNLVPVIVTVAERPS
                     RDVGLSLGYGTDDGFRAEAAYRDRDLFSRGYDLQSSLRLGSLQQIGYADVYMPPGLFP
                     FRGRDVPFRDSMGVLYEYNNIEKINLHRLAVAAYRHFTPEKFETRVGLSYQIERTHPE
                     GSDEQITRAMAPVIAFTWRRVDSLFDPQRGGVLNLQFASGWKSILSTQDFVKTYAQYQ
                     HWFPITPRDQLLARAEIGYTIAASRQGIPEDFLFRAGGSRSNRGYAYQSLGVREGEAT
                     VGGRYLMTASAEYVHWLNEKWGGAVFMDIGDAADTPKEWNSNRSYGIGARYKTPAGPL
                     ALDLAYAEGPSKFRISFSVTVAF"
BASE COUNT          327 a          800 c          803 g          329 t
ORIGIN      
        1 cgaggagatc gacgtccgcc acgcgcaggt tggcctcgat gccagacttc tgcacgacgg
       61 cctgcccgct cgccgcaccg ccccccggca gcgcgaccga caggtccgcg acatcgacgc
      121 gctccggcgt gaggaccacg cgcgccgagg ccgcgttgaa cggaagccgg cccttgtccc
      181 acggacccgg cgcatggttc tcgatgcgca ccgggccgga gaacgcgtca ccctgggaat
      241 cgagcttcgc ctgcacgtcg acgttggtgt ggagcgaccc ggcgatgcgc gcaagatcga
      301 gctccttcgc ggtcgcctcg agcgtgcgca gcggcatctt cgcgaacggc tcgaccgccg
      361 cgcgccccac cgcgtgctgg ccggagacgt cgccctcgaa cgtggcctcc aggcgcttca
      421 acgtgcccgc cgcgtgcagc tcgactgcat acggccgctc gccgaagctg ccctcgccgc
      481 gcgccttggc ctcgagcgcg aagggcgcgg tggtctcgag cgtgcccgcg agcgcggccc
      541 ggccgtaggt cgtttccgcg cgcgcttcgt ggatggtcca gcggctggcg tcgccctcgc
      601 cgcgcaacac gatgtcgcgc acgacgaagt cgcggtcgcg cgtcgcttcc atgagcgcgc
      661 gccgccgggc gacgtccttc tcggcgcgtg cctcgggcgt gagcgggccg atgcgcagct
      721 cgccgacgcg cccgtcctcg aggtcgacgg catagggcgg cttgaacgag accgggattt
      781 tcgcggcttc gcccgtcgag gccgtccgca ccaccaccga gcgcgcgacc agccggtgga
      841 cgaggagccg cccatgcagg ggctcgagcg gcgccgtgtc catctcgaag ccctcgacgc
      901 gcacgtacat gtccggccgg gagatctcga tcgtgtcgac gcgcagcagg ccgccgatgc
      961 gcccgtcgac gccggagaag gtcgtgcctt ccccgagcat ggtgctgacg cggccgagga
     1021 cgagccgcgc gccctcgggc gtcgtcatga cccaggtgat cgcgcccacg atgagcagca
     1081 acagcaccgc caccaccgca agcgtgcgcc agacccagcg catgcgccgc cggggtgtcg
     1141 gtgccggagg cggcgcggcg gcgggggctt cggcgctcat ggcttcagaa ggcgaccgtc
     1201 acggaaaacg agatgcggaa tttgctcggc ccctcggcat aggcgaggtc gagcgccagg
     1261 ggcccggccg gtgtcttgta gcgggcgccg atgccgtagc tgcgattcga gttccattcc
     1321 ttcggcgtat cggcggcgtc gccgatgtcc atgaagaccg cgccgcccca cttctcgttc
     1381 agccagtgca cgtactcggc gctcgccgtc atcaggtagc ggccgcccac cgtggcctcg
     1441 ccctctcgca cgccgaggct ctggtaggcg tagccgcgat tggagcgtga gccgccggca
     1501 cggaagagga agtcctccgg aatgccctgg cgcgacgcag cgatggtata gccgatctcc
     1561 gcgcgcgcca ggagctggtc gcgcggcgtg atcgggaacc agtgctggta ctgcgcatac
     1621 gtcttcacga aatcctgcgt ggacaggatc gacttccagc ccgaggcgaa ctgcaggttc
     1681 agcacgccgc cgcgctgcgg gtcgaagagg ctgtccaccc ggcgccaggt gaacgcgatc
     1741 accggcgcca tggcccgcgt gatctgctcg tccgagcctt cggggtgcgt ccgctcgatc
     1801 tggtacgaga ggccgacgcg cgtctcgaac ttctcgggcg tgaaatgccg gtaggcggcg
     1861 acggcgagcc ggtgcaggtt gatcttctcg atgttgttgt actcgtagag cacgcccatg
     1921 ctgtcgcgga acggcacgtc gcggccgcgg aaggggaaga ggcccggcgg catgtagacg
     1981 tccgcgtagc cgatctgctg caggctgccc agtcgcagcg agctctgcag gtcgtagccg
     2041 cgcgagaaga ggtcgcggtc gcggtacgcc gcctcggcgc ggaagccgtc gtcggtgccg
     2101 tagccgagcg agagccccac gtcgcgcgag gggcgctccg ccacggtcac gatgaccggg
     2161 accaggttcg gcttgagcgg atcgcgctcg acgtccaccg tgacggtcga gaaccacggg
     2221 cccgactgca ggcggcgctg cagctctccc agcgtgtcc
//
LOCUS       DATDMW010000300         5307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2208401, whole genome shotgun sequence.
ACCESSION   DATDMW010000300 DATDMW010000000
VERSION     DATDMW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2208401"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /locus_tag="VI319_12620"
     CDS             <1..1622
                     /locus_tag="VI319_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6864732.1"
                     /translation="PIPRVELPGKVYGTHPYVQNVRVPGMLHGRMVRPPAPDFVVEKV
                     DERAAKKLPGNVRVVAKRNFVGVVADREEQAIKAARALKIQWKQAIGLPAMKDMPAFT
                     RGTKSTEHAVASNGDVEAALKQARVHHAQYYVPHQMHASIGASCAVASVTADGATLWS
                     PTQSSFNLRDAVAKTLGLPAEKVHLIWAEGSGCYGHNGADDVTTDAAVLSQAVGKPVR
                     VQWMRHDEHGHEPKGAAMVMDYRGALDGKGNVAAWDSQVWSPGHAGRPSGDAAGNVLA
                     GAALGQPHKLAPTGADRNAKPTYVFPNARVRLQLLHSTVLRVSSLRGLGSPANTFGNE
                     SFIDELAAAAKADPIEFRIRHLKDERAVAVLEAVAKLAKWEPRPAPRRPKSGEGRGVA
                     FVHYDNYSGYAAVAMQVRVERASGKVRVERVAVAHDCGQIVNPDGLKNQIEGNIVQTL
                     SRALLEEVRFDTKKLTSLDWARYPILRFADLPQEIDIALLDRPDKPSLGAGEPAAAPV
                     MAAVANAIFDATGARLRNVPFTPEKVKAALV"
     gene            1643..2218
                     /locus_tag="VI319_12625"
     CDS             1643..2218
                     /locus_tag="VI319_12625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY6864733.1"
                     /translation="MATDPNYKILGMCGSLRAKSYNLMALKIAGGLMPAGMTLEIATI
                     GDLPLYNHDVQEKGFPAPVTRLRDQVLAADALLFASPEYNWSVSAPLKNVIDWLSRFK
                     PTVPFEGKPAAVISATQGPLGGARGQYDLRRILDGLGVQWLRKPEIFIGMAQNKIDAE
                     GRLLDEPTRKIMTEQMGAFRDWIGRVKRAYA"
     gene            2215..3165
                     /locus_tag="VI319_12630"
     CDS             2215..3165
                     /locus_tag="VI319_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY6864734.1"
                     /translation="MITEATVSYRLLSYQSGREPRAGILVEEQVYDPAGILGNAAYTS
                     VLAILQEWRTARSGLALAAERIIAGKSRAKGVPVKRVKLLAPVLHPGNIYCAGANYTD
                     HMIEMARAHGREPGPTAKERGDKPWHFIKASRSTIVGPGAKVRIPPYCRMLDWEVELA
                     AVVGRTAKDVPVEKALDFVAGYTIADDLSARDAGRREKTPETSPFHMDWLGHKSFDGS
                     CPLGPWITPAGDIPDPQNLGLKLWVNGELMQDSHTSKMIFSTAEQIAMLSSRVTLNPG
                     DLVLTGTPAGVGMPRKRFLNAGDSVKLWIEGIGEMTHTLA"
     gene            3191..3574
                     /gene="pcaC"
                     /locus_tag="VI319_12635"
     CDS             3191..3574
                     /gene="pcaC"
                     /locus_tag="VI319_12635"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEY6864735.1"
                     /translation="MSKENYDKGLAVRREVLGSDYVERQLATADDFNRPLQDLLTEYC
                     WGAVWARPQLPRKTRSLLNLAMLSALNRSTEFKTHVRGALKNGCTKDEIREVLLQVAI
                     YCGAPAGVEAFRLAREVFAEGEKRA"
     gene            complement(3590..4060)
                     /locus_tag="VI319_12640"
     CDS             complement(3590..4060)
                     /locus_tag="VI319_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864736.1"
                     /translation="MAAIVTGCAGYGSIAPGTGADQVRARMGAPAEVWKNADGTETWE
                     YPTSPNGWYTYMVDLGPDRAVRDVHQVLDEPYFSRIQAGQSREAVRRVLGAPKDVTRY
                     RGTNEEVWSWRYKEANNWYKLFNVAFDASSGTVLRTFRMDDPYMYDKGGRGKGG"
     gene            4154..5236
                     /locus_tag="VI319_12645"
     CDS             4154..5236
                     /locus_tag="VI319_12645"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HEY6864737.1"
                     /translation="MQRKIPCVLMRGGTSRGPYFLASDLPEDPGRRDQVLLRVMGSPH
                     PLQVDGLGGANTLTSKVAIVSRSKHSGADVDYLFAQVSIDQAFVDTKPNCGNMLAGVG
                     PFAIESGLVKPGGRETLVRIYNVNTETLIEAVIQTPGGSVEYEGDTRIDGVAEAAAPI
                     RLTFLDAYGAVTGKLLPTGEVTDRIQGVEVSIVDMAMPVAIMPAEAFGKTGGETAEAL
                     DADRDMMKRIESIRLEAGRRMGLGDVSKLVVPKPVLISSPSQGEGVRSRYFTPHACHK
                     AHAVTGALAVGTACALPGSVASRYVRPADFRGGVLRIEHPAGAIEVDLEVEDEGGTRN
                     VKRAALVRTARRIFEGSVLVPETLFS"
BASE COUNT          884 a         1807 c         1881 g          735 t
ORIGIN      
        1 agccgatccc gcgcgtcgag ctgccgggca aggtctacgg aacgcacccc tacgtgcaga
       61 acgtgcgcgt ccccgggatg ctgcacgggc gcatggtgcg gccgcccgcg cccgacttcg
      121 tcgtcgagaa ggtcgacgag cgcgcggcga agaagctccc gggcaacgtc cgcgtcgtgg
      181 cgaagcgcaa cttcgtgggc gtcgtcgcgg accgcgagga gcaggcgatc aaggccgcgc
      241 gcgccctgaa gatccagtgg aagcaggcga tcggcctgcc ggcgatgaag gacatgcccg
      301 cgttcacgcg cggcacgaag agcaccgagc acgcggtcgc ctcgaacggc gacgtcgagg
      361 ccgcgctcaa gcaggccagg gtgcaccacg cgcagtacta cgtgccgcac cagatgcacg
      421 cgtcgatcgg cgcctcgtgc gcggtcgcga gcgtgaccgc ggacggcgcg acgctctggt
      481 cgccgacgca gagctcgttc aacctgcgag acgcggtggc gaagacgctc gggcttccgg
      541 ccgagaaggt gcacctcatc tgggccgaag gctcgggctg ctacggccac aacggcgccg
      601 acgacgtcac caccgacgcg gcggtgcttt cgcaggcggt cggcaagccg gtgcgcgtgc
      661 agtggatgcg ccacgacgag cacggccacg agccgaaggg cgccgcgatg gtgatggact
      721 accgcggagc gctcgacggc aagggcaacg tcgccgcctg ggactcgcag gtgtggagcc
      781 ccggccacgc gggacggccg tcgggagacg ccgcgggcaa cgtgctcgcc ggcgccgcgc
      841 tcggccagcc gcacaagctc gcgccgaccg gcgccgaccg caacgcgaag ccgacctacg
      901 tatttcccaa tgcgcgcgtg cggctgcagc tgctgcactc gaccgtgctg cgcgtgtcgt
      961 cgctgcgcgg cctgggatcg cccgcgaaca ccttcggcaa cgagtcgttc atcgacgagc
     1021 tcgcggccgc cgcgaaggcc gacccgatcg agttccgcat ccgccacctg aaggacgagc
     1081 gcgcggtcgc cgtgctcgag gcggtggcga agctcgcgaa gtgggaaccg cggccggcgc
     1141 cgcgccgccc gaagagcggc gaagggcgcg gcgtcgcttt cgtgcactac gacaactaca
     1201 gcggctacgc tgcggtggcg atgcaggttc gggtggagcg cgcgagcggc aaggtgcgcg
     1261 tcgagcgcgt cgccgtcgcg cacgattgcg gccagatcgt gaacccggac ggcctgaaga
     1321 accagatcga aggcaacatc gtgcagacgc tctcgcgcgc gctgctcgaa gaggtgcgct
     1381 tcgacacgaa gaagctcacg agcctggact gggcgcggta tccgatcctg cgctttgccg
     1441 acctgccgca ggagatcgac atcgcgctcc tcgaccgccc cgacaagccg tcgctgggcg
     1501 cgggcgagcc ggcggcggct ccggtcatgg ctgccgtggc gaacgcgatc ttcgacgcga
     1561 ccggcgcgcg cctgcgcaac gtgccgttca cgccggagaa ggtcaaggcg gcgctggttt
     1621 aaccaaggag aacgagaaca acatggcgac ggaccccaac tacaagatcc tgggcatgtg
     1681 cggaagcctc agggccaagt cgtacaacct gatggcgctg aagatcgcgg gcggcctgat
     1741 gccggcgggc atgacgctcg agatcgcgac tatcggcgac ctgccgctct acaatcacga
     1801 cgtccaggag aagggctttc ccgcgccggt cacgcgcctg cgcgaccagg tgctggcggc
     1861 cgacgcgctg ctgttcgcgt cgcccgagta caactggtcg gtgtcggcgc cgctcaagaa
     1921 cgtgatcgac tggctctcgc gcttcaagcc gacggtgccg ttcgagggca agcccgcggc
     1981 ggtgatcagc gccacgcagg gaccgctcgg cggcgcgcgc ggtcagtacg acctgcgccg
     2041 catcctcgac gggctcgggg tgcagtggct gcgcaagccc gagatcttca tcggcatggc
     2101 gcagaacaag atcgacgccg agggcaggct gctcgacgag ccgacgcgca agatcatgac
     2161 cgagcagatg ggcgcgttcc gcgactggat cggccgcgtg aagcgggcgt acgcatgatt
     2221 accgaggcca ccgtgagcta ccgcctgctg agctatcagt cgggccgcga gccccgcgcc
     2281 gggatcctgg tcgaggagca ggtgtacgat cctgccggca tcctcggcaa cgccgcctac
     2341 accagcgtgc tcgcgatcct gcaggagtgg aggacggcgc gctcgggtct cgcgctcgcg
     2401 gccgagcgca tcatcgccgg caagagccgc gcgaagggcg tgccggtgaa gcgcgtgaag
     2461 ctgctcgcgc cggtgctcca cccgggcaac atctactgcg cgggggcgaa ctacaccgat
     2521 cacatgatcg agatggcgcg cgcgcacggg cgcgagcccg gtccgacggc gaaggagcgg
     2581 ggcgacaagc cctggcactt catcaaggcg tcgcgcagca cgatcgtcgg gcccggcgcg
     2641 aaggtgagga tcccgccgta ctgccgcatg ctggactggg aggtcgagct cgccgcggtc
     2701 gtcggccgca ctgcgaagga cgtcccggtc gagaaggcgc tcgacttcgt cgcaggctac
     2761 accatcgccg acgatctctc ggcgcgcgac gccggccggc gcgagaagac ccccgagacg
     2821 tcgccgttcc acatggactg gctcggccac aagtcgttcg acggatcctg cccgctcgga
     2881 ccgtggatca cgcccgcggg cgacattccc gatccgcaga atctcgggct caagctctgg
     2941 gtcaacggcg agctgatgca ggactcgcac acgagcaaga tgatcttcag caccgccgag
     3001 cagatcgcga tgctttcgtc gcgcgtgacg ttgaatccgg gcgacctggt cctcaccggt
     3061 acgccggcgg gcgtcggcat gccccgcaag cgctttctca acgccggcga ctcggtgaag
     3121 ctgtggatcg agggcatcgg cgagatgacc catacgttgg cgtaacgccg caatcacacg
     3181 gagacgcgaa atgtcgaagg agaactacga caagggcctg gcggtgcgca gggaagtgct
     3241 gggctcggac tacgtcgagc gccagctcgc gaccgcggac gacttcaacc ggccgctgca
     3301 ggacctgctc accgagtact gctggggcgc cgtctgggcc cggccccagc tcccgcgcaa
     3361 gacccgcagc ctgctgaacc tcgccatgct gtcggcgctc aaccgctcga ccgagttcaa
     3421 gacgcacgtg cggggcgcgc tgaagaacgg ctgcaccaag gacgagatcc gcgaggtgct
     3481 gctccaggtc gcgatctact gcggcgcgcc cgccggggtg gaggccttcc gcctcgcgcg
     3541 cgaggtgttt gccgagggcg agaaacgcgc ctgaacgtcg ccccgggagt cagccgccct
     3601 tgccgcggcc gcccttgtcg tacatatagg gatcgtccat gcggaaggtg cgcagcaccg
     3661 tcccgctcga cgcgtcgaag gcgacgttga acagcttgta ccagttgttc gcctccttgt
     3721 agcgccagga ccacacttcc tcgttcgtcc cccggtaccg ggtgacgtcc ttgggcgcgc
     3781 cgagcacgcg gcgcaccgcc tcgcgcgact ggcccgcctg gatcctggag aagtagggct
     3841 cgtcgagcac ctggtgcacg tcgcgcacgg cgcggtccgg tcccaggtcg accatgtagg
     3901 tgtaccagcc gttcgggctg gtcgggtact cccaggtctc ggtgccgtcg gcgttcttcc
     3961 agacctcggc gggagcgccc atgcgggcac ggacctggtc ggcgccggtc cccggggcga
     4021 tcgagccgta gccggcgcag ccggttacga tcgcggccaa ggccgcggcg gcggtggaaa
     4081 tgcgcaggaa ggtcatagtc ataaagttta gactacgcgt tgtgaaaaag cttcctcgag
     4141 acataaacac ccgatgcaac gaaaaattcc ctgcgttttg atgcgcggtg gcacctcgcg
     4201 ggggccctac ttcctcgcct ccgacctgcc cgaggacccc gggcgccgcg accaggtgct
     4261 gctgcgggtc atgggctcgc cgcacccgct ccaggtcgac ggcctgggcg gcgcaaatac
     4321 cctcaccagc aaggtggcga tcgtcagccg gtcgaagcat tccggcgcgg acgtcgacta
     4381 cctgttcgcg caggtgtcga tcgaccaggc cttcgtcgat acaaaaccga actgcggcaa
     4441 catgctcgcc ggcgtcgggc cattcgcgat cgagtcgggg ctggtgaagc cgggcgggcg
     4501 cgagacgctc gtgcgcatct acaacgtcaa caccgagacg ctgatcgaag ccgtgatcca
     4561 gacgccgggc ggctcggtcg agtacgaagg cgacacgcgc atcgacggcg tcgccgaggc
     4621 ggcggcgccg atcaggctca cgtttctcga cgcgtacggc gccgttaccg gaaagcttct
     4681 ccccacgggc gaggtgacgg accggatcca gggcgtcgag gtcagcatcg tcgacatggc
     4741 gatgccggtc gcgatcatgc ccgccgaggc cttcggcaag accggcggcg agaccgccga
     4801 ggcgctcgac gccgatcgcg acatgatgaa gcggatcgag tcgatccgcc tcgaggccgg
     4861 ccggcgcatg ggcctcggcg atgtctcgaa gctcgtcgtg ccgaagccgg tgctgatctc
     4921 gagcccgtcg cagggcgagg gcgtgcgctc gcgctatttc acgccgcacg cctgccacaa
     4981 ggcgcacgcc gtgacggggg cgctcgcggt gggcaccgcg tgcgcgctgc ccggctcggt
     5041 ggcgagccgc tacgtgcgtc cggcggactt ccgcggcggc gtgctgcgga tcgagcaccc
     5101 ggccggcgcc atcgaggtcg acctcgaggt cgaggacgag ggcgggacgc gcaacgtgaa
     5161 gcgcgccgcg ctggtgcgca cggcgcgccg catcttcgaa ggcagcgtgc tcgttcccga
     5221 gacgctgttc agctaggcgg ccttcgtcgc cgccggccgc gacgccgaga gcagctcgcg
     5281 tagcacgaac ggcaggatgc cgccgtg
//
LOCUS       DATDMW010000301         2512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_588316, whole genome shotgun sequence.
ACCESSION   DATDMW010000301 DATDMW010000000
VERSION     DATDMW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588316"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VI319_12650"
     CDS             complement(<1..390)
                     /locus_tag="VI319_12650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920328.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HEY6864738.1"
                     /translation="MLDYGPDALILKGLDGDNWRLDDYVKRGGYSALKKLLADKTPPE
                     NVVAELKKSALRGRGGAGFPTGLKWSFMPKNFQGPKYLVCNSDEGEPGTFKDRDILRY
                     NPHIVIEGMAIGAYAMGIPVGYNYIHGE"
     gene            complement(390..863)
                     /gene="nuoE"
                     /locus_tag="VI319_12655"
     CDS             complement(390..863)
                     /gene="nuoE"
                     /locus_tag="VI319_12655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY6864739.1"
                     /translation="MLSPEALTKIDRETAKYPPEHKRSAVMAALAVAQDEHGHLSTEV
                     MDAVAQHLGMAPVAVYEVATFYTMYDLDPTGKYKLCICTNLPCALSGAEEAAEHLKKK
                     LGVDWNETSADGRFTLKQGECFGACGDAPVILVNNKKLLSFMTPEKLDQFIAESK"
     gene            complement(875..2128)
                     /locus_tag="VI319_12660"
     CDS             complement(875..2128)
                     /locus_tag="VI319_12660"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY6864740.1"
                     /translation="MAEIRNYTLNFGPQHPAAHGVLRLVLELDGEVIMRADPHIGLLH
                     RATEKLAENKTYVQALPYMDRLDYVSMMSNEHAYCMAVERLLGVEVPIRAQYIRVMFD
                     ELTRILNHLLWLGAHALDIGAMTVFLYAFREREDLMDVYEAVSGARMHAAYYRPGGVY
                     RDLPEAMPKYKASMYKNDSDVRKLNEAREGSLLDFIEDFTNRFPKYVDEYETLLTDNR
                     IWKQRTVNIGVVTPERALALGFTGPMLRGSGIAWALRKKQPYEVYDRMEFDIPVGVNG
                     DCYDRYLVRIEEMRQANRIVKQCIDWLRGNPGPVITDNHKIAPPSRVEMKSNMEELIH
                     HFKLFTEGIHVPPGEVYAAVEHPKGEFGIYLVSDGANMPYRMKIRAAGFPHLAALDEM
                     ARGHMLADVVAIIGTQDIVFGEIDR"
     gene            complement(2128..>2512)
                     /locus_tag="VI319_12665"
     CDS             complement(2128..>2512)
                     /locus_tag="VI319_12665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY6864741.1"
                     /translation="RFGVVSHLLSVTRNRRLWLRVFAENDDFPVLDSLTSVWPCANWY
                     EREAFDLYGIVFTGHADLRRILTDYGFVGHPFRKDFPLSGNVEMRYDVEQKRVIYQPV
                     TIERRVVTPRVIREDQYGDLGPGKR"
BASE COUNT          397 a          819 c          827 g          469 t
ORIGIN      
        1 ctcgccgtgg atatagttgt agccgaccgg gatgcccatg gcgtacgcgc cgatggccat
       61 gccttcgatg acgatgtgcg ggttgtagcg caggatgtcg cggtccttga acgttcccgg
      121 ctcgccctcg tcggaattgc agacgaggta cttgggaccc tggaagttct tcggcatgaa
      181 gctccacttc agtcccgtgg ggaagcccgc gccaccgcgc ccgcgcagcg cggacttctt
      241 cagctcggcg accacgttct cgggcggagt cttgtcggcg agcagcttct tcagcgccga
      301 gtagccgccg cgcttcacgt agtcgtcgag acgccagttg tcgccgtcga ggcccttgag
      361 gatgagggcg tcgggaccgt agtcgagcat cacttcgact ccgcgatgaa ttgatcgagc
      421 ttctcggggg tcatgaacga gagcagcttc ttgttgttca cgaggatcac gggggcgtcg
      481 ccgcaggcgc cgaagcactc gccctgcttc agcgtgaagc ggccgtcggc actggtctcg
      541 ttccagtcga cgcccagctt cttcttcagg tgctcggccg cctcctccgc gcccgagagc
      601 gcgcacggca ggttcgtgca gatgcacagc ttgtacttgc cggtcggatc gaggtcgtac
      661 atggtgtaga acgtcgccac ctcgtagacc gcgaccggcg ccatgccgag gtgctgcgcg
      721 acggcgtcca tgacctcggt cgacaggtgg ccgtgctcgt cctgcgcgac ggcgagcgcc
      781 gccatcacgg cggagcgctt gtgctcgggc ggatacttcg cggtctcccg gtcgatcttc
      841 gtcagggctt ccgggctgag caaggtcgcg gccatcagcg gtcgatctct ccgaaaacga
      901 tgtcctgcgt gccgatgatg gcgacgacgt cggcgagcat gtgtccgcgc gccatctcgt
      961 cgagcgccgc caggtgcggg aatcccgcgg ctcggatctt catgcggtag ggcatgttcg
     1021 cgccgtcgga gacgaggtag atgccgaact cgcccttggg atgctcgacc gcggcgtaga
     1081 cctctcccgg cggcacgtgg atgccctcgg tgaagagctt gaagtggtgg atcagctctt
     1141 ccatgttgct cttcatctcc acccgcgacg gcggagcgat cttgtggttg tcggtgatca
     1201 cgggccccgg gttgccgcgc agccagtcga tgcactgctt cacgatgcgg ttcgcctggc
     1261 gcatctcctc gatgcgcacc aggtaccggt cgtagcaatc gccattgacg ccgaccggga
     1321 tatcgaactc catgcggtcg tacacctcgt acggctgctt cttgcgcagg gcccaggcga
     1381 tgcccgagcc gcgcagcatc ggcccggtga acccgagcgc gagcgcgcgc tccggcgtca
     1441 ccacgccgat gttgaccgtg cgctgcttcc agatgcggtt gtcggtgagc agcgtctcgt
     1501 actcgtcgac gtacttcggg aaccggttgg tgaagtcctc gatgaaatcg agcagcgagc
     1561 cctcgcgggc ctcgttcagc ttgcgcacgt cgctatcgtt cttgtacatc gacgccttgt
     1621 acttcggcat ggcctcgggc agatcgcggt agacgccgcc cggccggtag taggccgcgt
     1681 gcatgcgcgc ccccgacacc gcctcgtaga cgtccatcag gtcctcgcgc tcccggaacg
     1741 catacaggaa caccgtcatc gcgccgatat cgagcgcgtg cgccccgagc cacagcaggt
     1801 gattcaggat ccgcgtcagc tcgtcgaaca tcacgcggat gtactgcgcc cggatcggca
     1861 cctcgacccc gagcaggcgc tcaaccgcca tgcagtatgc gtgctcgttc gacatcatcg
     1921 agacgtagtc gaggcgatcc atgtaaggca gcgcctggac gtaggtcttg ttctcggcga
     1981 gcttctccgt cgcccggtgc agcagcccga tgtgcgggtc ggcgcgcatg atcacctcgc
     2041 cgtccagctc gagcaccagg cgcagcacgc cgtgcgcggc ggggtgctgc gggccgaagt
     2101 tgagcgtgta gttgcggatc tcggccatca gcgttttccc gggccgaggt cgccgtactg
     2161 gtcctcgcga atcacccgcg gggtgaccac gcgccgctcg atcgtcacgg gctggtagat
     2221 gaccctcttc tgctcgacgt cgtagcgcat ctcgacgttt ccggaaagcg ggaagtcctt
     2281 gcggaacgga tgacccacga agccgtagtc ggtgaggatg cggcgcaggt cggcgtggcc
     2341 ggtgaagacg atgccgtaga ggtcgaacgc ctcgcgctcg taccagttgg cgcacggcca
     2401 caccgacgtc agcgaatcga gcaccggaaa atcgtcgttc tcggcgaaca cgcgcagcca
     2461 caggcgccgg ttccgcgtga ccgacagcag gtgcgagacg accccaaagc gg
//
LOCUS       DATDMW010000302         9413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_91792, whole genome shotgun sequence.
ACCESSION   DATDMW010000302 DATDMW010000000
VERSION     DATDMW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91792"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VI319_12670"
     CDS             <1..501
                     /locus_tag="VI319_12670"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEY6864742.1"
                     /translation="IVHYEERLVARLHGFHRRFCLWSRVNRGSMQKPGLVLGLDAGGS
                     CHGVAYRIAARHATEELRLLWRREMVLGAYTPRWVPIDAGGTALRAIAFVVNRRHPSY
                     AGKLPLETVIRTLVSARGYLGTPAEYLLETVKGLIEHGVRDSYLMELRKRILQMHPEL
                     APAQAR"
     gene            506..1606
                     /gene="hppD"
                     /locus_tag="VI319_12675"
     CDS             506..1606
                     /gene="hppD"
                     /locus_tag="VI319_12675"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY6864743.1"
                     /translation="MEHDRAENPMSNPMGTEGFEFVEYAAPDPRALGALFETLGFKAI
                     ARHRHKNVILYRQGGVNFIVNAEPDSFAQRFARLHGPSICAIALRVRDAAAAYRRAID
                     MGAWGFDSRSGPMELNIPAIKGVGDSLIYFVDRWRGKDGRCGIGNISIYDVDFEQLGA
                     DGDGSHAGAGLLAVDHLTHNVHRGRMKEWADFYERLFNFREARYFDIEGRLTGLKSKA
                     MISPCGKIRIPINESSDDRSQIQEYLEAYHGEGIQHIALATADIHATVESLRRAGVAF
                     MDTPDTYYEALDARVPGHGEDVGRLRRDRILLDGAPGDLLLQIFTKTVIGPIFFEIIQ
                     RKGNEGFGEGNFRALFESIELDQIRRGSLKPA"
     gene            1632..2969
                     /gene="hmgA"
                     /locus_tag="VI319_12680"
     CDS             1632..2969
                     /gene="hmgA"
                     /locus_tag="VI319_12680"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY6864744.1"
                     /translation="MDARVRKRPKSRARKPGAELAYQSGFGNEHASESVPGALPAGRN
                     SPQRAPHGLYAELLSGTAFTAPRAANRRTWTYRIRPSATHEPFERLQHPTLRTAPFDE
                     GVATPSQLRWGPFPAPTRPADFVDGLATIAGNGDARLQCGMAAHWYAANRDMKDRVFY
                     DADGELLIVPQAGALELFTELGRLAVAPGEIALVPRGMRFRVELPQGRACGYVCENYG
                     SMFRLPELGPIGSNGLASPRDFLAPVAAYEDVDRRHEIVAKFGGALWSAGIDHSPLDV
                     VAWHGSLAPYKYDLARFMVVGTVSFDHPDPSIYTVLTSPSDTAGWANVDFVIFPPRWL
                     VAEDTFRPPWYHRNVMSEFMGLVHGVYDAKAEGFLPGGASLHNSCSAHGPDAETFGRA
                     SSAKLAPHKIEDTLAFMFESRYPIRATRYAMDTHPPQRDYAQCWRGLRKRFRP"
     gene            2966..3964
                     /locus_tag="VI319_12685"
     CDS             2966..3964
                     /locus_tag="VI319_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY6864745.1"
                     /translation="MKLGTLKDDSRDGALLVVSRDLRHATVAYDVVPTLQAALDDWDY
                     ASPQLQTLYETLNRSPQALGSRAFELEPQRLAAPLPRAYQWLDGSAYLAHVELVRKAR
                     GASMPAEFKRDPLMYQGDSPWFVGPCDPIEAASEDWGIDLEAEVAVFLDDVPMGVKAD
                     RASEHVRLVALVNDISLRNLIPAELAKGFGFVHGKAATAFSPVAVTPDELGEAWDGRR
                     LKLPMRVSVNDKPFGSPDCGTDMQFGFHQLIAHAAATRPLGAGTVLGSGTISNYGYEA
                     GHACIAELRALETVKKGEPQTPFLRFGDRVRIEMLDAAGNSVFGAIDQSVSAPAVP"
     gene            complement(4017..4172)
                     /locus_tag="VI319_12690"
     CDS             complement(4017..4172)
                     /locus_tag="VI319_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864746.1"
                     /translation="MCYEYEWYEKARLAEQARRLKEKAKEDRRSAEPLAPVKPVPARP
                     VKEQVPA"
     gene            complement(4290..5510)
                     /gene="phaZ"
                     /locus_tag="VI319_12695"
     CDS             complement(4290..5510)
                     /gene="phaZ"
                     /locus_tag="VI319_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY6864747.1"
                     /translation="MIYALNELRLAALAPWNAAAQTTLDLFTSPFSPFAYLPESRRLA
                     AGAEVFCRLTQRYEKPAFGIRDTLAGGVRVGVEERVAIEKPFCSLLRFCKPEARAEQP
                     KVLLVAPLSGHHATLLRDTVRALLPDHEVYVTDWTDARMVPIAHGPFHLDDYVDYIRE
                     FIRYLGPDVHVISVCQPTVPVLAAVALMADAGERVAPRTMVMMGGPIDARRSPTAVND
                     LATRRPMSWFERSVIDKVPAKYPGFMRRVYPGFLQHLGFVAMNPRRHVWAHWDFYNHL
                     VEGDGESAEAHRAFYDEYNAVLDMPAEYYLDTIRTVFKEHRLAHGTWKVRGDLVRPEA
                     IERTALLTIEGELDDISGNGQTEAAQGLCSGIPARKREHLLVPKVGHYGIFSGRRWRE
                     AISPHIGQFIRKHA"
     gene            5677..6051
                     /locus_tag="VI319_12700"
     CDS             5677..6051
                     /locus_tag="VI319_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864748.1"
                     /translation="MARILIIDDDRTFVELLRVHLSDAGHDADVAGDAVAGLRFILAD
                     PPDLLLLDLNIPYLHGFEILRALKNDPLTRRMPVVIITGRDDEDAYVQALQIGIDGYL
                     TKPVQREMLLATVETALGPVKA"
     gene            complement(6041..7735)
                     /locus_tag="VI319_12705"
     CDS             complement(6041..7735)
                     /locus_tag="VI319_12705"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEY6864749.1"
                     /translation="MQKIWLSSYPPGVPAEIDYSGNRSVGELFEKNAAKFQDRPAFYN
                     MGTTITFGELDRRSRDFAAWLQGRGLRKGARVAVMMPNCLQYPIAAFGILRAGCVVVN
                     VNPLYTSRELEHQLKDAGAEAIVILENFCHVLAEVVARTPVRHVAVAALGDLLGAKGL
                     LVNFVLRHVKKMVPAWSIEGTVRFNGALAEGARRELAKVDVGHEDVAFLQYTGGTTGV
                     AKGATLTHRNILANLEQSFAWIKPFLDESQPQIIITALPLYHILSLTANCLFMLKIGG
                     CNILITNPRDIPAFVKELAKHRFTMFTGVNTLFNALLNNPGFAKLDFSGIKVALGGGM
                     AVQKSVADRWKQVTGSTLVEAYGLSETSPAVTINPLDLAAYNGAIGLPIPSTEVELRG
                     DDGKPVPLGQPGEICVRGPQVMRGYWNRPDETAKVIDSRGFFATGDIGVMDEKGYVRI
                     VDRKKDMIIVSGFKVFPNELEAVIAAHPGVLECAVIGVPDEHSGEVPKVFVVRKDPAL
                     TEADLRRYFQENLTGYKRPKYVEFRKELPKTNVGKILRRALRDEARAAPQTEPVRL"
     gene            complement(7860..9068)
                     /locus_tag="VI319_12710"
     CDS             complement(7860..9068)
                     /locus_tag="VI319_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6864750.1"
                     /translation="MKGKPQVIVAGAGPVGLLTALALAARDIPVLVLEAEPNLTIDLR
                     AGTYHPPSLEMMAPYGITDEMHKTAIRVPRWQIRDRREGVIVEWDVTEIADLTPYPYR
                     LHLEQHRLTPIIFAKLKAYPNAEVRFSHEVSDVEQTADRVVVTAKTPGGEEKFEAPWV
                     VGADGGRSTVRKCVGIDFEGFTWPERFVVVSTTDDYARHGYAMNSYVSDPREWVAVFK
                     MPDDGPPGMWRVIFPVPEDEEEEITLSEQLVEKRMQGFVPREGRYPIKYKSIYKVHQR
                     VARDFRAGRVLLAGDAAHLNNPMGAFGLNGGIQDSFNLTEKLGKVCRGEAGPELLDLY
                     VRQRRTVNLEYVQEFSIKNLRRLNEKDERERQKNFAEIRQQAETLEGRREFLKISSMI
                     ASVQRANAIR"
     gene            complement(9065..>9413)
                     /locus_tag="VI319_12715"
     CDS             complement(9065..>9413)
                     /locus_tag="VI319_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6864751.1"
                     /translation="IATLGDGAYIFANPTAGHWVANAYELPILVVVFNNSLWGAVRRA
                     TSGMYKGGVSARDDWRGLASLAPAPAYEKLVEAHGGHGERVERAEDLPAALERALAAT
                     RKGQQALLNVICE"
BASE COUNT         1434 a         3274 c         3162 g         1543 t
ORIGIN      
        1 atcgtgcact acgaggagcg cctggtcgcg cgtctgcacg gcttccatcg gcgcttctgc
       61 ctttggtcgc gcgtgaaccg cggctcgatg cagaagccgg ggctggtcct cggcctcgat
      121 gcgggcggca gctgtcacgg cgtcgcgtac cggatcgccg cccggcatgc gaccgaagag
      181 ctgcgcctgc tctggcggcg cgagatggtg ctcggcgcgt acacgccgcg ctgggttccg
      241 atcgacgccg gaggcacggc gctgcgtgcg atcgcgttcg tggtgaaccg gcggcatccg
      301 agctacgccg ggaagcttcc gctggagacc gtgatccgta cgctggtgag cgcgcgcggc
      361 tatctcggca caccggccga atacctgctc gagaccgtga agggcctgat cgagcacggc
      421 gtccgagatt cgtatctcat ggagctgcgc aagcggatcc tgcagatgca tcccgagctc
      481 gcgccggcgc aggcgcgttg agaccatgga acacgatcga gccgagaacc cgatgagcaa
      541 cccgatgggc accgagggct tcgaattcgt cgagtacgcg gcccccgacc cgcgcgcgct
      601 cggcgcgctg ttcgagacgc tcggcttcaa ggcgatcgcg cgccaccgcc acaagaacgt
      661 catcctctac cgccagggcg gcgtcaactt cattgtcaac gccgagcccg actcgttcgc
      721 gcagcgcttc gcgcgcctgc acgggccgtc gatctgcgcg atcgcgctcc gcgtgcgcga
      781 cgcggcggcc gcgtaccggc gcgcgatcga tatgggcgcg tggggcttcg actcgcgctc
      841 cggcccgatg gagctcaaca tccccgcgat caagggcgtc ggcgactcgc tcatctactt
      901 cgtcgaccgc tggcgcggca aggacggccg gtgcggcatc ggcaacatct ccatctacga
      961 cgtcgacttc gagcagctcg gggccgatgg cgacggctcg cacgccggcg cggggctgct
     1021 cgcggtcgac cacctgacgc acaacgtgca ccgcggccgc atgaaggagt gggccgactt
     1081 ctacgagcgg ctgttcaact tccgcgaggc gcgctacttc gacatcgagg gccgtctcac
     1141 cggcctgaag agcaaggcga tgatcagccc gtgcggaaag atccgcatcc cgatcaacga
     1201 gtcctcggac gaccgctcgc agatccagga atacctcgag gcgtaccacg gcgagggcat
     1261 tcagcacatc gcgctcgcca ccgccgacat ccacgcgacc gtcgagtcgc tacgccgcgc
     1321 gggcgtcgcg ttcatggata cgcccgacac gtattacgag gcgctcgacg cgcgcgtgcc
     1381 cgggcacggc gaggacgtcg ggcgcctgcg gcgcgaccgg atcctcctcg acggagcgcc
     1441 cggcgacctg ctgctccaga tcttcaccaa gacggtgatc ggcccgatct tcttcgagat
     1501 catccagcgc aagggcaacg agggcttcgg cgaaggcaat ttccgcgcgc tgttcgagtc
     1561 gatcgagctc gaccagatcc ggcgtggctc gctgaaaccc gcttgacaac gacgctcggg
     1621 gaggtgaaac gatggacgcc cgcgtgagga aacggccgaa gtcccgtgcc cgtaagccgg
     1681 gcgcggagct cgcgtaccag agcggcttcg gcaacgagca cgcgagcgag tcggtcccgg
     1741 gcgcgctgcc ggcggggcgc aacagcccgc agcgggctcc tcacggcctc tacgccgagc
     1801 tcctctcggg gaccgcgttc accgcgccgc gcgcggcgaa ccggcgcacg tggacgtacc
     1861 gtatccgccc gtccgcgacg cacgagccgt tcgagcggct gcagcatccg acgctgcgca
     1921 ccgcgccctt cgacgaggga gtcgcgacgc cgtcgcagct gcgctggggc ccgttcccgg
     1981 ctccgacgcg gccggccgat ttcgtggacg gcctcgcgac gatcgcgggc aacggcgacg
     2041 cgcgcctgca gtgcgggatg gcggcgcact ggtacgccgc gaaccgcgac atgaaggatc
     2101 gcgtcttcta cgacgccgac ggcgagctgc tgatcgttcc gcaggcgggg gcgctcgagc
     2161 tcttcaccga gctcggccgc ctcgcggtcg cgcccggcga gatcgcgctc gtcccgcgcg
     2221 ggatgcgctt ccgcgtcgag ctgccgcagg gacgcgcctg cggctacgtc tgcgagaact
     2281 acggctcgat gttccgtttg cccgagctcg ggccgatcgg ctcgaacggg ctcgcgagcc
     2341 cgcgcgactt cctcgcgccg gtcgccgcgt acgaggacgt cgaccggcgc cacgagatcg
     2401 tcgcaaagtt cggcggcgcg ctctggtccg cgggcatcga ccactcgccg ctcgacgtcg
     2461 tcgcctggca cgggagcctg gcgccttaca agtacgacct cgcccggttc atggtcgtcg
     2521 gcacggtgag cttcgaccat cccgacccct cgatctacac ggtgctgacc tcgccttcgg
     2581 acaccgcggg ctgggccaac gtggacttcg tcatctttcc gccgcgctgg ctggtcgccg
     2641 aggacacgtt ccggccgccg tggtatcacc gcaacgtgat gagcgagttc atggggctgg
     2701 tgcacggcgt atacgacgcc aaggccgagg gcttcctgcc gggcggcgcg agcctgcaca
     2761 actcatgcag cgctcacggg cccgacgccg agaccttcgg gcgcgcgtcg tcggcgaagc
     2821 tggccccgca caaaatcgag gatacgctcg cgttcatgtt cgagtcgcgc tacccgatcc
     2881 gggcgacgcg ctatgcgatg gacacgcacc cgccgcagcg cgattacgcg cagtgctggc
     2941 gcgggctcag gaagcgcttt cggccatgaa gctcgggacc ctcaaggacg actcgcgcga
     3001 cggcgcgctg ctcgtcgtca gccgcgacct caggcatgcg acggtcgcct acgacgtcgt
     3061 cccgacgctg caggctgcgc tggacgactg ggactacgcg tcgccgcagc tgcagacgct
     3121 gtacgagacg ctgaaccgct cgccgcaggc gctcggctcg cgcgcattcg agctcgagcc
     3181 gcagcggctc gcggcgccgc tgccgcgcgc ctaccagtgg ctcgacggca gcgcgtacct
     3241 cgcgcacgtg gagctggtgc gcaaggcccg cggcgcgtcc atgcccgcgg agttcaagcg
     3301 cgacccgctc atgtaccagg gcgactcgcc gtggttcgtc gggccgtgcg accccatcga
     3361 ggccgcgagc gaggactggg gcatcgacct cgaggccgag gtggccgtct ttctcgacga
     3421 cgtgccgatg ggcgtgaagg ccgaccgggc gagcgagcac gtgcgcctgg tcgcgctcgt
     3481 gaacgacatc agcctgcgca acctcatccc ggccgagctc gccaagggct tcggcttcgt
     3541 gcacggcaag gcggcgaccg cgttctctcc ggtcgcggtg acccccgacg agctcggcga
     3601 ggcctgggac gggcgccgcc tcaagctgcc aatgcgcgtg agcgtcaacg acaagccgtt
     3661 cggatcgccg gattgcggca ccgacatgca gttcgggttt caccagctga tcgcgcacgc
     3721 cgcggcgacg cggccgctcg gcgcggggac ggtgctcggc tcgggcacga tctcgaacta
     3781 cggctacgag gcggggcatg cctgcatcgc cgagctgcgc gccctcgaga cggtgaagaa
     3841 aggcgagccg cagacgcctt tcctcaggtt cggcgaccgc gtccgcatcg agatgctcga
     3901 cgcggccggg aactcggtct tcggagctat cgaccagtcg gtctccgccc ccgcggttcc
     3961 ctagaaaagc aaagccccgc gaacgcgggg ctttgctctc gatcgtgcct ggagcctcag
     4021 gcggggactt gctccttcac cgggcgagcc ggaacgggct tcaccggggc caggggctcc
     4081 gcggacctgc ggtcttcctt ggctttctcc ttgagtcggc gggcctgctc ggcgagcctc
     4141 gctttctcgt accattcgta ttcgtagcac atgccaaact cctttttcgg atcaggtcga
     4201 cgcccctccc tgccggcttc ttggagttgt gaaagctatg ctacgcgcgg ccaccgccga
     4261 aggcaatata gaaagccgcc cggccggtgt caggcgtgct tccggatgaa ctgcccgatg
     4321 tgcggactga tcgcctcgcg ccagcgccgg cccgaaaaga tcccgtagtg cccgaccttg
     4381 ggcacgagca agtgctcacg tttccgggcc gggattcccg agcacaggcc ctgcgccgcc
     4441 tcggtctgcc cgttgcccga gatatcgtcg agctcgcctt cgatggtgag cagggcggtg
     4501 cgctcgatcg cttccgggcg caccaggtcg ccccggacct tccacgtccc gtgcgccagg
     4561 cggtgctcct tgaagacggt gcggatggtg tcgaggtagt actccgcggg catgtcgagc
     4621 accgcgttgt actcgtcata gaaggcgcgg tgcgcctcgg ccgactcgcc gtcgccctcg
     4681 acgagatggt tgtagaagtc ccagtgcgcc cagacgtgcc ggcgtggatt catcgcgacg
     4741 aacccgaggt gctgcaggaa tcccggatac acgcggcgca tgaagccggg atacttcgcc
     4801 ggcaccttgt cgatcaccga gcgctcgaac caggacatcg gcctgcgcgt cgcgaggtcg
     4861 ttcaccgccg tgggactgcg tcgcgcgtcg atcgggccgc ccatcatgac catcgtccgc
     4921 ggcgcgacgc gctcgccggc gtcggccatc agcgcgaccg ccgcgagcac cggcaccgtc
     4981 ggctggcaca ccgagatcac gtgcacgtcg ggccccaggt aacggatgaa ctcgcggatg
     5041 tagtcgacgt agtcgtcgag gtggaacggc ccgtgcgcga tcggcaccat gcgcgcgtcg
     5101 gtccagtcgg tcacgtacac ctcgtgatcg ggcagcagcg cacgcaccgt gtcgcgcagc
     5161 agcgtcgcgt ggtgcccgga cagcggcgcg acgagcagca ccttcggctg ctccgcgcgc
     5221 gcctcgggct tgcagaagcg cagcaggctg cagaagggct tctcgatggc aacgcgctct
     5281 tccaccccga cgcgcacgcc gccggcgagc gtatcgcgga tcccgaacgc gggcttctcg
     5341 tagcgctggg tcaggcggca gaacacttcg gcgccggcgg ccaggcgccg gctctcgggc
     5401 aggtaggcga acgggctgaa agggctggtg aagagatcga gcgtggtctg ggctgcggca
     5461 ttccagggtg cgagcgcggc gagtcgaagt tcgttcagcg cgtagatcat cccgtcctcc
     5521 cgaaaaacgg ctgtcgtctt ataccagacc ggagccgcga accgcaatgc aggtttgcaa
     5581 atgaggtcat attccacagg catgaaaacg catccgctgc ttcgcctcgt cgcgcggtgc
     5641 tagcatgtcg gcagcccatt cgctttcggt cccaccgtgg cccgcatact gatcatcgat
     5701 gatgaccgca cgttcgtcga gctcctgcgc gtgcatctgt ccgatgccgg tcacgacgcc
     5761 gacgtcgcgg gcgacgcggt ggccgggttg cggttcatcc tggcggaccc gcccgacctg
     5821 ctgctgctcg atttgaatat tccgtatctg cacggcttcg agatcctgcg cgcgctgaag
     5881 aacgatccgc tgacgcgcag gatgccggtc gtgatcatca ccggccggga cgacgaggac
     5941 gcctacgtcc aggccctgca gatcgggatc gacggttacc tcaccaagcc cgtgcagcgg
     6001 gagatgctgc tcgcgacggt cgaaaccgcg ctcggcccgg tcaaagcctg accggctcgg
     6061 tctgcggcgc ggcgcgcgcc tcgtcgcgca gcgcccgcct caggatcttg ccgacgttgg
     6121 tcttgggcag ctccttgcgg aactcgacgt acttcgggcg cttgtacccg gtcaggttct
     6181 cctggaagta ccggcgcagg tcggcctcgg tgagtgccgg atccttgcgc acgacgaaga
     6241 ccttcggtac ctcgcccgaa tgctcgtccg gcacgccgat gaccgcgcac tcgagcactc
     6301 cgggatgcgc cgcgatcacc gcctcgagct cgttcgggaa gaccttgaac cccgagacga
     6361 tgatcatgtc cttcttgcgg tcgacgatgc gcacgtatcc cttctcgtcc atcacgccga
     6421 tgtcgccggt ggcgaagaac ccgcgcgagt cgatcacctt cgccgtttcg tccgggcggt
     6481 tccagtaccc gcgcatcacc tgcgggccgc gcacgcagat ctcgcccggc tgcccgagcg
     6541 gcacgggctt gccgtcgtcg ccgcgcagct cgacctcggt cgaggggatc ggcagcccga
     6601 tcgcgccgtt gtaggccgcg aggtcgagcg gattgatcgt gacggcagga gacgtctccg
     6661 acagtccgta ggcctccacc agggtcgagc cggtcacctg cttccaccgg tcggcgacgg
     6721 acttctggac cgccatgccg ccgccgagcg cgaccttgat cccggagaag tcgagcttcg
     6781 cgaaaccggg gttgttgagc agcgcgttga acagcgtgtt gacgccggtg aacatcgtga
     6841 accggtgctt cgccagctcc ttaacgaatg ccgggatgtc gcgcggattg gtgatgagga
     6901 tgttgcagcc gccgatcttg agcatgaaca ggcagttcgc agtcagcgac aggatgtggt
     6961 acagcggcag cgccgtgatg atgatctgcg gctgcgattc gtcgaggaac ggcttgatcc
     7021 aggcgaagct ctgctccagg ttggccagga tgttgcggtg ggtcagcgtt gcgcccttcg
     7081 ccacgccggt cgtcccgccc gtgtattgca ggaaggccac gtcctcgtgg ccgacgtcga
     7141 ccttcgccag ctcgcgccgg gcgccttcgg caagcgcgcc gttgaaccgc accgtgccct
     7201 cgatgctcca ggcgggcacc atcttcttga cgtgccgcag gacgaagttg accagcaggc
     7261 ccttcgcgcc gagcaggtcc ccgagcgcgg ccaccgcgac gtggcgcacc ggcgtgcgcg
     7321 cgacgacctc ggcgagcacg tgacagaagt tctcgaggat cacgatcgcc tcggcgcccg
     7381 cgtccttgag ctgatgctcg agctcgcggg acgtgtacag gggattgacg ttgacgacga
     7441 cgcacccggc ccgcaggatc ccgaacgccg cgatcgggta ctgcaggcag ttcggcatca
     7501 tgaccgcgac gcgcgcgcct ttgcgcagtc cgcggccctg cagccaggcc gcgaagtcgc
     7561 gcgacctccg gtcgagctcg ccgaaggtga tggtggtccc catgttgtag aacgcggggc
     7621 ggtcctggaa cttcgccgcg ttcttctcga acagctcgcc gaccgagcgg ttcccgctgt
     7681 agtcgatctc ggccggcacg ccgggcgggt agctgctcag ccagatcttc tgcatatggg
     7741 acccctccta ttgcagcgcg gggtcgccga acgacccggg ccactgctgg cttcactgca
     7801 taagttaagg gagaaacccg atccgttcaa atccgcccac acgagccggc gcgcaagcgt
     7861 cagcgaatgg cgttcgcccg ctgcacgctc gcgatcatgc tgctgatctt caggaactcg
     7921 cggcgacctt ccagcgtttc cgcctgctgc ctgatctcgg cgaagttctt ctgccgctcg
     7981 cgctcgtcct tctcgttcag gcgccgcagg ttcttgatcg agaactcctg cacgtactcg
     8041 aggttcacag tgcggcgctg gcgcacgtac agatcgagca gctcgggacc ggcctcgccg
     8101 cggcatactt tcccgagctt ctcggtcagg ttgaacgagt cctggatgcc tccgttcagc
     8161 ccgaaggctc ccatcgggtt gttgaggtgc gccgcgtctc ccgcgagcag cacgcgcccg
     8221 gccctgaagt ccctggccac acgctggtgc accttgtaga tgctcttgta cttgatgggg
     8281 tagcggccct cgcgcggcac gaatccctgc atgcgcttct cgaccagctg ctcggacagc
     8341 gtgatctcct cctcctcgtc ctcggggacc gggaagatca cgcgccacat gcccggcggc
     8401 ccgtcgtcgg gcatcttgaa cacggcgacc cattcgcggg gatccgagac gtacgagttc
     8461 atggcgtagc cgtgccgcgc gtagtcgtcc gtggtgctca cgacgacgaa gcgctccggc
     8521 caggtgaaac cctcgaagtc gatgccgacg cacttgcgca ccgtgctgcg gccgccgtcg
     8581 gcgcccacga cccacggcgc ctcgaacttc tcctcgcctc ccggcgtctt cgccgtcacg
     8641 acgacgcggt cggcggtctg ctcgacgtcg ctcacctcgt gcgagaagcg cacctcggcg
     8701 ttgggatacg ccttgagctt ggcgaagatg atcggcgtca gccgatgctg ctccagatgc
     8761 aggcggtacg ggtacggcgt gaggtcggcg atctcggtca cgtcccattc gacgatgacg
     8821 ccttccctgc ggtcgcggat ctgccagcgc ggcacccgga tcgcggtctt gtgcatctcg
     8881 tcggtgatgc cgtacggcgc catcatctcg agcgagggcg ggtgataggt gccggcgcgc
     8941 aggtcgatgg tgaggttggg ctcggcctcg aggaccagca ccgggatgtc gcgcgcggcg
     9001 agcgcgagcg ccgtcaacag gccgaccggc ccggcgccgg ccacgatgac ctgcggcttg
     9061 cccttcattc gcagatcacg ttcagcagcg cctgctgccc cttgcgggtg gccgccagcg
     9121 cgcgctcgag cgccgcgggg agatcctcgg ctcgctccac gcgctcgccg tggccaccgt
     9181 gcgcctcgac cagcttctcg tacgcaggcg cgggcgcgag gctcgcgagc ccgcgccagt
     9241 cgtcccgggc ggacacgccg cccttgtaca tgccgctcgt cgcgcggcgc accgcgcccc
     9301 aaagcgagtt gttgaacacg accacgagga tcggcagctc gtaggcgttc gccacccagt
     9361 ggcctgcggt gggattggcg aagatgtagg cgccgtcgcc gagcgtcgcg atc
//
LOCUS       DATDMW010000303        10819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1711481, whole genome shotgun sequence.
ACCESSION   DATDMW010000303 DATDMW010000000
VERSION     DATDMW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10819
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711481"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2044
                     /locus_tag="VI319_12720"
     CDS             <1..2044
                     /locus_tag="VI319_12720"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY6864752.1"
                     /translation="GTKRAVGVVIDVASGSDVAPERLRRVERILRDELRLPAEWLDFA
                     RFASDYYHEPLGQVILNALPPRLRGPKAPPAPPRAFATTEAGRAALATLPRRNARQRA
                     LLERLASGPVDAAQMSPPERSQLSRALEAGWIEPRAPVATRARFIAAHELNAEQRAAV
                     EAVAEGFGVTVLFGVTGSGKTEVYLWLIARALDAGRQALVLVPEIALTPALETAFRER
                     FPGARLVVQTSAMPEVERAASWLAALEGRADIVLGTRLAVFAPLARLGLVVVDEEQDA
                     SFKQQEGLRYSARDLAIVRARSAKAPVILCSATPSVETFHHALSGRYRLIELHRRAIP
                     EARLPAIRLLDLRQHPAREGLAAPLIEAIGARLARGEQALVFLNRRGYAPVLACPACG
                     WVSGCTRCSANMVLHLADRELRCHHCGLAHGVPRTCPQCGNPDLQPFGRGTQRIESTL
                     AERFPSARVLRIDRDSARGRAGLEGLLGRAHRREADILVGTQILAKGHHFGRLTLVGV
                     INADAGLFSPDYRAGERSFAQLQQVAGRAGRAELPGEVLIQTRYPHHPLYQSLVRHDY
                     AGFAKSVLAEREGAGFPPFVFEAALRAESRDAERALDFLRRAASLAPASDGAVTLYDP
                     APMSLARLGGMERAQMLAQSPSRPRLQAFLREWTARLYAERASGVRWHLDVDPTEF"
     gene            complement(2053..2157)
                     /locus_tag="VI319_12725"
     CDS             complement(2053..2157)
                     /locus_tag="VI319_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEY6864753.1"
                     /translation="MGKGDKRTFKGKVFKGSHGNTRRRKAKKKAPAKK"
     gene            2251..3993
                     /gene="argS"
                     /locus_tag="VI319_12730"
     CDS             2251..3993
                     /gene="argS"
                     /locus_tag="VI319_12730"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY6864754.1"
                     /translation="MSAEIRSHLTGLFARALAQIAPQQPASLITLERPKQAQHGDFSC
                     PVAMQLAKSLKRSPREVAQALLAALPASDWVERAEIAGPGFINVRLRPAAKQHVVRTV
                     LAAGERYGRSDAAGGERVMVEFVSANPTGPLHVGHGRQAALGDAISALLEWRGSRVTR
                     EFYYNDAGNQIENLALSVQARARERLGLASEFPADGYHGEYIGEIAAKYLASGARDAE
                     DLDAIRRFAVAELRAEQDRDLQAFGVRFDNYYLESSLYADGRVERTVKSLVDSGETFE
                     RDGALWLKTTDYGDDKDRVMRKSDGTYTYFVPDVAYHVAKWERGFHRVINVQGSDHHS
                     TVVRVRAGLQAISEKQGLGIPAGYPDYVLHKMVTVMKGGAEMKISKRAGSYVTLRDLI
                     DEVGRDATRFFLVSRKPDTEFVFDVDLAKSQSEENPVYYVQMAHARISSVLEQWRTQF
                     AVEDGSLFPTSAPNDPSDALNDVDLGPLTGEREFALLKSVGDFPDAIEAACQDLEPHQ
                     IAFTLRELAGQFHSYYNAERILISDAPLRRARLALCAAVRQVLRNGLGLLGVSAPNKM
                     MREDAPFSAPGTGP"
     gene            3998..4597
                     /locus_tag="VI319_12735"
     CDS             3998..4597
                     /locus_tag="VI319_12735"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY6864755.1"
                     /translation="MQQDFKPRPAPRSKSGGTLLLGMVVGFLLGLAVAALIAVYFLKT
                     PMPFLEKGKEKPPGAPNLTEGAKPPEKAQQAKADTKPRFDFYRILPGQEEPVTERQVR
                     EAQRQAQKTGEPPKETYLLQAGSFQNPADADNLKAKLALIGVEANVEPTNLAEKGMWY
                     RVRVGPYTKLEDINRVRQQMVENGIQVSLVRIKDATAKN"
     gene            4605..5243
                     /locus_tag="VI319_12740"
     CDS             4605..5243
                     /locus_tag="VI319_12740"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY6864756.1"
                     /translation="MIKKLFAFAAVLSFALPALAQRAPEEGMDYNELRPAQQTESPGK
                     IEVTEFFWYRCPHCYALEPQLASWLKQLPKDVQFRRVPAIFNDEWAIDARIFYTLDTM
                     GQEERVHRALFDAIHQQGGVRLKGEAYAKWVADWVAKQGVDAKKYDETYRSFSVQTKV
                     RRATQMTNAYKFDGVPAIAVQGRWLVNASNTMLSTTSYLIDQSRKGAATARK"
     gene            5257..6042
                     /locus_tag="VI319_12745"
     CDS             5257..6042
                     /locus_tag="VI319_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6864757.1"
                     /translation="MSAAGAGLRVFVTGASSGIGAALASHYARQGAGALGLVARRLDR
                     LEALAARLGVPAACYGADVTDADAMRAAGADFVARFGAPDIVVANAGVSAGTLIEHAE
                     DLAALRRILEINVAGVANTFQPFIEPMRARGRGTLVGIASVAGFRGLPGAGAYSASKA
                     AAIAYLESLRVELRASGIAVVTICPGYIATPMTAGNPYRMPFLLEADEAARRFARAIA
                     AKRRRVVIPWQMALLSLVFRALPGWLYDRVFQLGPRKPRRILD"
     gene            complement(6055..7767)
                     /gene="phaC"
                     /locus_tag="VI319_12750"
     CDS             complement(6055..7767)
                     /gene="phaC"
                     /locus_tag="VI319_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY6864758.1"
                     /translation="MDLAAAMFANPWKLAELQMKLAWDYAALWQSSMLRLMGKESRPV
                     AEPAKGDNRFRDVEWQDNFVFDYLKQSYLIAARHIHDAVTGTEGLSDDTRKKVNFFTR
                     QYIDALAPSNFALTNPQVLRETLASGGQNLVRGLNNLLGDIERGDGQLRISMTDEGAF
                     KLGENVATTPGKVVFQNELIQLIQYAPTTQTVFRRPLLVIPPWINKYYILDLREKNSF
                     IRWAVGEGHTVFVVSWVNPDARYADKGFDDYMLEGSLAAIGAVCKATGEADVNVIGYC
                     LGGTLLGATLAYMAEKSDTRAASATFFVSLLDFSIPGELGVFIDEPQVRSLERRMNER
                     GYLEGSEMAATFNLLRANDLIWSFVINNYLLGKEPFPFDLLYWNADSTRMPAKMHSFY
                     LRKMYIENKLKDPGGVTLAGVPIDLRRISLPAYFISTVEDHIAPWKSTYKGARILAGA
                     TGPRSRPAGPVRFVLGGSGHIAGIVNPPSAKKYGYWTNADLPESPDAWHQAAGWHEGS
                     WWSDWQAWIAALNGKERVPARVPGEGALKAIEDAPGSYVRVRTGAEKPEPLPAVEGEK
                     EPLR"
     gene            complement(7933..8958)
                     /locus_tag="VI319_12755"
     CDS             complement(7933..8958)
                     /locus_tag="VI319_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6864759.1"
                     /translation="MTPESLLQYPQATPPAAAEVRSIAPGVRWLRMPLPFALDHINLW
                     LLEDQEAWTLVDCGLGNDATRAIWERLLADVREPLRRIVVTPHHPDHVGQAAWLLARS
                     GAELWMPQAEFLTAHVLREGLAGYAFDNVLAMFRANGLGGERLAGIEGRGMAYRRGVP
                     ELPQTYRRLAAGERLRIGGREWQVLPGYGHSPEHASLWCEQTGVFIAGDMVLPRISTN
                     VSVWGVDPLGNPLALFLESIERFAALPADTLVLPSHGLPFRGLRERIAQLVEHHRLRL
                     AELHEACDVPKAAAEVLGTLFRRELDAHQTFFAMGEAMAHLHYLHADGRLRRERGSDS
                     IIRYVQA"
     gene            9058..9459
                     /locus_tag="VI319_12760"
     CDS             9058..9459
                     /locus_tag="VI319_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY6864760.1"
                     /translation="MTETAQTYTIGALAREFDVTPRAIRFYEDEGLIAPGRTAGRRVY
                     GKRDYVRLKLILRGKRLGFSLAEVREMLELYDAAPDERPQLHKFLAALQARREQLESQ
                     RRDIDEVLAEIRGFEAQSRKLLDGRRRRKRG"
     gene            9521..10684
                     /locus_tag="VI319_12765"
     CDS             9521..10684
                     /locus_tag="VI319_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY6864761.1"
                     /translation="MEIPGLRFALGDDVEMLRASVRDFAAAEIAPRAAEIDRSNQFPA
                     DLWRKLGALGLLGVTAEEEYGGTMMGYTAHIVAMEEISRASGSVGLSYGAHSNLCVNQ
                     IRRNGSDAQKRRYLPKLVSGEAVGALAMSEPGAGSDVVSMRTRAEYRSGRYILNGSKM
                     WITNGPDADVLVVYAKTDAEAAARGITAFIVEKGFPGFSTAQKLDKLGMRGSNTCELV
                     FRDCAVPEENVLSEPGRGVNVLMSGLDYERAVLAGGPLGIMQACLDVVVPYLHERKQF
                     GQPIGEFQLMQGKLADMYVTLNASRAYVYAVGRACDRGEVTRKDAAGAILFASEKATT
                     MALEAIQCLGGMGYINDSPTGRLLRDAKLYEIGAGTSEIRRWLIGRELFDETK"
BASE COUNT         1667 a         3739 c         3770 g         1643 t
ORIGIN      
        1 cggtacgaag cgcgccgtcg gcgtggtgat cgacgtcgcg tccggctccg acgtggcgcc
       61 cgagcggctg cgccgggtcg agcgcatcct gcgcgacgag ctgcggcttc ccgccgagtg
      121 gctcgatttc gcccggttcg cgagcgacta ctatcacgag ccgctcggcc aggtgatcct
      181 gaacgcgctg ccgccgcggc tgcgcggacc gaaggcgccg cccgctccgc cgcgtgcgtt
      241 cgcgacgacc gaagcgggcc gggcggcgct tgcaacgctt cccaggcgca acgcgcgcca
      301 gcgagcgctg ctcgagcgtc tcgcgagcgg ccccgtcgac gcggcccaga tgtctccgcc
      361 cgagcgctcg cagctgtcgc gcgcgctcga ggcggggtgg atcgagccgc gagcgccggt
      421 cgcgacgcgg gcgcgcttca tcgcggcgca cgagctgaac gcggagcagc gcgccgcggt
      481 cgaggccgtg gccgaaggtt tcggcgtgac cgtcctgttc ggggtcacgg gcagcggcaa
      541 gaccgaggtc tacctgtggc tcatcgcgcg cgcgctcgac gcgggccggc aggcgctggt
      601 cctcgtgccc gagatcgcgc tcacgcccgc gctcgagacg gcgttccgcg agcgcttccc
      661 cggggcgcgg ctcgtggtgc agacgagcgc gatgcccgag gtcgagcgcg ccgcgagttg
      721 gctggccgcc ctcgaaggcc gcgccgacat cgtgctcggc acgcggctcg cggtgttcgc
      781 gccgctcgcg cggctcgggc tggtggtggt ggacgaggag caggacgcct ccttcaagca
      841 gcaggagggc ctgcgctact cggcgcgcga cctcgcgatc gtgcgcgcgc gatcggcgaa
      901 ggcgcccgtc atcctgtgct cggcgacgcc ctcggtcgag accttccacc acgcgctctc
      961 gggacgctac cggctgatcg agctgcaccg ccgcgcgatc cccgaggcgc gcctgccggc
     1021 gatccgcctg ctcgacctgc gccagcaccc ggcgcgcgaa gggctcgccg ccccgctcat
     1081 cgaggcgatc ggcgcgcggc tcgcgcgcgg cgagcaggcg ctcgtcttcc tcaaccgccg
     1141 cggctatgcg ccggtgctcg cgtgccccgc gtgcggatgg gtgagcggct gcacgcgctg
     1201 cagtgcgaac atggtgctgc acctcgccga ccgcgagctg cgctgccacc actgcggcct
     1261 cgcgcacggc gtgccacgca cctgcccaca gtgcgggaac cccgacctgc agcccttcgg
     1321 ccgcgggacg cagcgcatcg agtcgacgct cgccgagcgc ttcccgagtg cgcgcgtgct
     1381 gcgcatcgac cgcgattcag cgcgcggtcg ggccgggctc gaaggtctgc tgggccgcgc
     1441 ccaccgccgc gaggccgaca tcctggtcgg cacgcagatc ctcgcgaagg ggcaccactt
     1501 cggacggctg acgctggtcg gcgtgatcaa cgccgacgcc ggcctgttct cccccgacta
     1561 ccgcgcgggc gagcgcagct tcgctcagct gcagcaggtc gcggggcggg ccggacgcgc
     1621 cgagctgccg ggcgaggtgc tcatccagac gcgctatccg caccacccgc tctaccagtc
     1681 gctcgtgcgc catgactacg ccggcttcgc gaagagcgtg ctcgccgagc gcgagggagc
     1741 gggtttcccg ccgttcgtgt tcgaggccgc gctgcgcgcg gagagccgcg atgccgagcg
     1801 cgcgctcgac ttcctgcgcc gcgcggcgtc gctcgcgccc gcgtcggacg gcgcggtgac
     1861 cctctacgac cccgcgccca tgtcgctcgc ccgcctcggc ggcatggagc gcgcgcagat
     1921 gctcgcgcaa tctccgtcgc gcccgcggct gcaggccttc ctgcgcgagt ggacagcgcg
     1981 cctgtacgcc gagcgcgcga gcggcgtgcg ctggcacctc gacgtggatc cgaccgagtt
     2041 ctagtcgggg gtttacttct tcgccggcgc ttttttcttc gccttgcgcc ggcgggtgtt
     2101 gccgtgcgag cctttgaaga ccttgccctt gaaggtgcgc ttgtcgcctt tgcccatgct
     2161 ttcctctcgc tgcggtccga acggagtcgg cccgtataat aagcggtttg ccgtgaagcc
     2221 acgctttcaa gcggccttat cgcgtcgttt atgtccgccg agatacgctc ccacctcacc
     2281 gggctcttcg ccagggcgct cgcacagatc gcgccgcaac agccggcctc gctgatcacg
     2341 ctcgagcgcc cgaagcaggc gcagcacggc gacttctcgt gcccggtggc gatgcagctc
     2401 gcgaagagcc tcaagcgcag cccgcgcgag gtggcgcagg cgctgctcgc ggcgctgccc
     2461 gcgtccgact gggtcgagcg cgccgagatc gcgggtccgg gattcatcaa cgtgcggctg
     2521 cgcccggcgg cgaagcagca cgtcgtgcgc accgtgctcg cggcgggcga gcgctatggg
     2581 cgctcggacg ccgccggcgg cgagcgcgtc atggtcgagt tcgtctcggc gaacccgacc
     2641 gggccgctgc acgtcggcca cggccggcag gccgcgctcg gcgacgcgat ctcggcgctg
     2701 ctcgaatggc ggggctcgcg cgtgacgcgc gagttctact acaacgacgc gggcaaccag
     2761 atcgagaacc tcgcgctgtc ggtgcaggcg cgcgcgcgcg agcgcctcgg gctcgcgtcc
     2821 gaattccccg ccgacggcta tcacggcgag tacatcggcg agatcgccgc gaaatatctc
     2881 gcctcgggcg cgcgcgacgc cgaggacctc gacgcgatcc ggcgcttcgc ggtcgcggag
     2941 ctgcgtgccg agcaggatcg cgacctgcag gccttcggcg tgcgcttcga caactactat
     3001 ctcgagtcgt cgctctacgc cgacggccgc gtcgagcgca cggtgaagag cctcgtcgac
     3061 tcgggcgaga ccttcgagcg cgacggcgcc ctatggctga agaccaccga ctacggggac
     3121 gacaaggacc gcgtcatgcg caagagcgac ggcacgtaca cgtacttcgt gcccgacgtc
     3181 gcctaccacg tcgcgaaatg ggagcgcggc ttccatcggg tgatcaacgt gcaggggtcg
     3241 gatcaccaca gcaccgtggt gcgagtgcgc gccggcttgc aggcgatctc ggaaaagcag
     3301 gggctcggga ttcccgccgg ataccccgac tacgtgctgc acaagatggt gaccgtcatg
     3361 aaaggcggcg ccgagatgaa gatctccaag cgcgccgggt cctacgtgac gctgcgcgac
     3421 ctgatcgacg aggtcggccg cgacgcgacg cgattcttcc tggtgtcgcg gaagcccgac
     3481 accgagttcg tgttcgacgt cgacctggcg aagtcgcagt cggaggagaa cccggtgtac
     3541 tacgtgcaga tggcgcacgc ccgcatatcg tccgtcctgg agcaatggcg tacgcaattc
     3601 gccgttgaag acggcagtct tttcccgaca agcgcgccca atgacccgag cgacgcgctg
     3661 aatgacgtcg atctcggtcc cctgacgggg gaacgggaat tcgcgctgct caaaagcgtg
     3721 ggcgacttcc cggacgcgat cgaggcggcg tgtcaagacc ttgagccgca tcagatcgct
     3781 tttacattgc gcgaactggc aggccagttc cacagttact ataatgccga acgcatcctg
     3841 atctcggatg cgccgctgcg ccgggcgcga ctcgctctct gtgcggccgt ccggcaggtc
     3901 ctgcgcaacg gtctgggctt gctgggcgtg agcgcaccca acaagatgat gcgggaagat
     3961 gcaccgttct ctgctcctgg aacaggccct taaccgaatg cagcaagatt tcaagcccag
     4021 gcccgcgccg cgatcgaagt cgggcggcac gctcctgctc ggcatggtgg tcggattcct
     4081 gctcggtctc gcggtcgccg cgctgatcgc ggtctatttc ctgaagacgc cgatgccttt
     4141 cctcgagaag ggcaaggaga agccgcccgg cgcgcccaac ctgaccgagg gagcgaagcc
     4201 gcccgagaag gcgcagcagg cgaaagcgga caccaagccg cgcttcgact tctaccgcat
     4261 cctgcccggc caggaggagc ccgtgaccga gcgccaggtg cgcgaggcgc agcgccaggc
     4321 gcagaagacc ggcgagccgc cgaaggagac gtacctgctc caggccggtt cgttccagaa
     4381 tccggccgac gccgacaatc tcaaggccaa gctcgcgctc atcggcgtcg aggccaacgt
     4441 cgagcccacg aacctcgccg agaagggcat gtggtaccgc gtgcgcgtcg ggccttatac
     4501 gaagctcgag gacatcaacc gcgtccgtca gcagatggtc gagaacggga tccaggtctc
     4561 tctcgttcgc atcaaggacg cgaccgccaa gaactgaggg aagcatgatc aagaaactgt
     4621 tcgcattcgc cgccgtcctg tccttcgcgc tgcctgcgct cgcgcagcgc gcgcccgagg
     4681 aagggatgga ttacaacgag ctccggcccg cgcagcagac cgagtcgccg ggcaagatcg
     4741 aggtcaccga gttcttctgg taccgctgcc cgcactgcta cgcgctcgag ccgcagctcg
     4801 cgtcgtggct gaagcagctg cccaaggacg tgcagttccg gcgcgtgccc gcaatattca
     4861 acgacgagtg ggcgatcgac gcccgcatct tctacacgct cgacacgatg ggccaggagg
     4921 agcgcgtgca ccgggcgctc ttcgacgcga tccaccagca gggcggcgtg cgcctgaaag
     4981 gcgaggccta cgccaagtgg gtcgccgact gggtcgcgaa gcagggcgtc gacgcgaaga
     5041 agtacgacga gacctaccgc tccttctcgg tccagaccaa ggtgcggcgc gccacgcaga
     5101 tgaccaacgc ctacaagttc gacggcgtgc ccgcaatcgc cgtgcaggga cgctggctcg
     5161 tgaacgcgtc gaacacgatg ctgtccacga ccagctacct catcgaccag tcgcgcaagg
     5221 gcgcggcgac cgccaggaag tagcctccgt ccttccgtga gcgcggccgg cgcagggctc
     5281 cgggtcttcg tcaccggggc ctcgagcggc atcggcgccg cgctcgcctc gcattacgca
     5341 cgacaaggcg ccggcgcgct ggggctcgtc gcccggcgtc tcgaccggct cgaggccctc
     5401 gccgcgcggc tcggcgtgcc cgcggcctgt tacggcgcgg acgtgaccga cgcggacgcg
     5461 atgcgcgccg cgggcgccga cttcgtcgcc cgtttcggcg cgcccgacat cgtcgtcgcc
     5521 aacgccggcg tctcggccgg tacgctcatc gagcacgccg aggacctcgc ggcgctgcgc
     5581 cgcatcctcg agatcaacgt cgcgggagtc gccaacacct tccagccgtt catcgagccg
     5641 atgcgcgcac gcggccgcgg aacgctggtc gggatcgcga gcgtcgccgg cttccgcggg
     5701 ctgccgggcg cgggagcgta cagcgcctcg aaagccgcgg cgatcgccta tctcgagagc
     5761 ctgcgggtcg agctgcgcgc gagcggcatc gcggtggtca cgatctgccc cggctacatc
     5821 gcgacgccga tgaccgccgg gaatccttac cgcatgccgt tcctgctgga agccgacgag
     5881 gcggcgcggc gcttcgcgcg cgcgatcgcg gcgaagcgcc ggcgcgtggt cattccctgg
     5941 cagatggcct tgctctcgct cgtgttccgg gcgctgcccg ggtggctcta cgaccgcgtg
     6001 ttccagctcg gaccgcgcaa gccacgccgc attttggact aggtggtcaa ctagctagcg
     6061 cagcggctcc ttctcgccct cgaccgcggg cagcggctcg ggcttctcgg cgccggtgcg
     6121 cacgcgcacg tacgagcccg gcgcgtcctc gatggccttg agcgcccctt caccgggaac
     6181 gcgggccggc acgcgctcct tgccgttcag cgccgcgatc cacgcctgcc agtcgctcca
     6241 ccaggagcct tcgtgccacc cggcagcctg gtgccaggcg tcgggcgact ctggcaggtc
     6301 ggcgttggtc cagtagccgt acttcttggc ggacggcgga ttgacgatgc cggcgatgtg
     6361 gcccgagccg ccgagaacga atcggaccgg gccagcgggt cgagagcgcg ggcccgtcgc
     6421 gccggcgagg atgcgcgcgc ccttgtacgt cgacttccag ggggcgatat ggtcctcgac
     6481 ggtcgagatg aagtacgcgg gcagcgagat cctgcgcagg tcgatcggca cgccggcgag
     6541 cgtgacgccg cccgggtcct tgagcttgtt ctcgatgtac atcttgcgca ggtagaagct
     6601 gtgcatcttc gccggcatgc gcgtggaatc ggcgttccag tacagcaggt cgaacgggaa
     6661 cggctccttg ccgaggaggt agttgttgat cacgaacgac cagatcaggt cgttcgcgcg
     6721 cagcaggttg aaggtcgccg ccatctccga gccctcgagg tagccgcgct cgttcatgcg
     6781 gcgctcgagg ctccgcacct gcggctcgtc gatgaagacg cccagctcgc ccgggatcga
     6841 gaagtcgagc agcgagacga agaaggtcgc cgacgccgcg cgcgtgtcgc tcttctcggc
     6901 catgtaggcg agggtcgcgc cgagcagcgt gccgcccagg cagtagccga tgacgttgac
     6961 gtcggcctcg cccgtcgcct tgcacacggc gccgatcgcg gcgagcgatc cctcgagcat
     7021 atagtcgtcg aagcccttgt cggcgtagcg cgcgtccggg ttcacccagg agaccacgaa
     7081 caccgtgtgc ccctcgccca ccgcccagcg gatgaaggaa ttcttctcgc gcaggtcgag
     7141 gatgtaatat ttgttgatcc acggcgggat gacgaggagc gggcgcctga acacggtctg
     7201 cgtggtcggc gcgtactgga tcagctggat cagctcgttc tggaacacga ccttgcccgg
     7261 cgtggtcgcg acgttctcgc cgagcttgaa cgcaccctcg tcggtcatgc tgatgcgcag
     7321 ctggccgtcg ccgcgctcga tgtcgccgag aagattgttc agcccgcgca ccaggttctg
     7381 cccgcccgat gcgagcgtct cgcgcagcac ctgcgggttg gtgagcgcga agttggacgg
     7441 cgccagcgcg tcgatgtact ggcgcgtgaa gaagttgacc ttcttgcggg tgtcgtcgga
     7501 cagtccttcg gtgccggtca ccgcgtcgtg gatgtggcgc gcggcgatga gataggactg
     7561 cttgaggtag tcgaagacga agttgtcctg ccattcgacg tcgcggaagc gattgtcgcc
     7621 cttcgcgggc tccgcgaccg gcctgctctc cttgcccatc agccgcagca tcgacgactg
     7681 ccagagcgcc gcgtagtccc acgcgagctt catctgcagc tcggcgagct tccacgggtt
     7741 cgcgaacatc gccgcggcga ggtccatgaa cgccttggcg atgccgaact cgtcgctgag
     7801 cgcgacgccc ttgccgtcgg cgtggcgctg tgcgaagtcg gcgaggagct tgcccgagcg
     7861 ctcggcgacc gcggcgaaga tgcgcgcggt ctgtgcaggg tcgggggcgg gtttctgctg
     7921 tgcggacctg ggtcaggcct gaacgtaacg gatgatgctg tcggaaccgc gctcgcgtcg
     7981 caggcggccg tcggcgtgga gatagtgcag gtgggccatc gcctcgccca tggcgaagaa
     8041 ggtctggtgc gcatcgagct cgcgccggaa cagcgtcccg agcacctcgg ccgcggcctt
     8101 cgggacgtcg catgcctcgt gcagctcggc gaggcgcagg cggtggtgct cgaccagctg
     8161 cgcgatgcgc tcgcgcaggc cgcggaacgg cagcccgtgc gagggcagca ccagcgtgtc
     8221 ggcggggagc gcggcgaagc gctcgatcga ctcgaggaag agcgcgaggg ggttgcccag
     8281 cggatcgacg ccccagacgc tgacattggt cgagatccgc ggcagaacca tgtcgcccgc
     8341 gatgaagacg ccggtctgct cgcaccacag cgacgcgtgc tcgggcgaat gcccatatcc
     8401 gggcagcacc tgccattcgc gcccgccgat ccgcaggcgc tcgcccgcgg cgaggcgccg
     8461 gtaggtctgc ggcagctcgg gcacgccgcg ccggtacgcc atgccgcggc cttcgatgcc
     8521 ggcgaggcgc tcgccgccga gcccgttcgc gcggaacatc gcgagcacgt tgtcgaaggc
     8581 gtagccggcg agcccttcgc gcaggacgtg cgcggtgagg aactcggcct gcggcatcca
     8641 gagctcagcg cccgagcggg cgagcagcca cgccgcctgg ccgacgtggt ccggatggtg
     8701 gggggtgacg acgatccggc gtagcggctc ccgcacgtcg gccagaaggc gctcccagat
     8761 cgctcgtgtc gcgtcgttgc cgaggccgca gtcgacgagc gtccaagcct cctggtcctc
     8821 gagcagccac aagttgatat ggtcgagcgc gaacggcagc ggcatgcgca gccagcgcac
     8881 gcccggggcg atgctgcgga cctccgcggc agcgggcggc gtcgcttgcg ggtactgcag
     8941 cagggactcc ggtgtcatcg cctcttatat ggggccgcgg cgaccggagt aaaattcccg
     9001 cctcgcgacg ccaaaacgct gaattttcgc agattcgcgc cgcccgcgcc atcgaccatg
     9061 accgaaacgg cccagaccta tacgatcggc gccctcgcgc gcgagttcga cgtgacgccg
     9121 cgcgcgatcc gcttctacga ggacgagggg ctgatcgcgc ccggacgcac ggcgggccgc
     9181 cgcgtgtacg gcaagcgcga ctacgtgcgg ctgaagctga tcctgcgcgg caagcgtctc
     9241 gggttctcgc tcgccgaggt gcgcgagatg ctcgagctct acgacgcggc tcccgacgag
     9301 cggccgcagc tgcacaagtt cctggcggcg ttgcaggcgc gccgcgagca gctcgagagc
     9361 cagcgccgcg acatcgacga ggtgctcgcc gagatccgcg gcttcgaggc gcagtcgcgc
     9421 aagctcctgg acggccgccg gcgccgcaag cgcggctgag ccgcgggccg ccggcgcgca
     9481 attgacgtta acgttaacgt taagtacagt gattcccccc atggaaatcc ccggactgcg
     9541 cttcgccctg ggcgacgacg tcgagatgct gcgcgcgagc gtgcgcgact tcgccgcggc
     9601 cgagatcgcc ccgcgcgcgg ccgagatcga ccgctccaac cagttcccgg cggacctgtg
     9661 gaggaagctc ggcgcgctgg ggctgctcgg agtcacggcc gaggaagaat acggcgggac
     9721 gatgatgggc tacaccgcgc acatcgtcgc gatggaggag atcagccgcg cctccggctc
     9781 ggtcggcctc tcctacggcg cgcactcgaa cctgtgcgtg aaccagatcc gcaggaacgg
     9841 cagcgacgcg cagaagcgca ggtacctgcc gaagctcgtc tcgggcgagg cggtcggggc
     9901 gctcgcgatg tcggagccgg gcgcggggtc cgacgtggtc tcgatgcgca cgcgcgccga
     9961 atatcgcagc ggccggtaca tcctgaacgg cagcaaaatg tggatcacca acggccccga
    10021 cgccgacgtg ctggtcgtct atgccaagac cgacgccgag gcggccgccc gtggaatcac
    10081 cgccttcatc gtcgaaaagg gcttccccgg cttctccacc gcgcagaagc tcgacaagct
    10141 gggcatgcgc ggctcgaaca cctgcgagct ggtctttcgc gactgcgccg tgccggagga
    10201 gaacgtgctc tccgagccgg ggcgcggcgt caacgtcctc atgagcggac tggactacga
    10261 gcgcgccgtg ctcgcgggcg ggccgctggg gatcatgcag gcatgcctgg acgtggtcgt
    10321 accctatttg cacgagcgca agcagttcgg gcagccaatc ggcgagttcc agctgatgca
    10381 gggcaagctc gccgatatgt acgtgacgtt gaacgccagc cgcgcctacg tttacgcggt
    10441 gggccgcgcc tgcgaccgcg gcgaggtcac gcgcaaggat gcggccggcg ccatcctgtt
    10501 cgcgtcagag aaggcgacta cgatggccct ggaagccatc cagtgcttgg gcggcatggg
    10561 ctacattaac gactcgccca ccggccggct gctgcgcgac gccaagctct acgaaattgg
    10621 agccggcacc tccgaaatcc ggcgctggct aatcggccgc gaattgttcg acgaaacgaa
    10681 ataggcgccg gcatgaggaa tctgaggggg aaatcacggc agatttcctc tttgaagtga
    10741 ccactaacta acgttgaatt cacagggaaa atggcccagt tgcacacccc cctctgcgac
    10801 ctgctgggat tgcgcctgc
//
LOCUS       DATDMW010000304        19712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1586407, whole genome shotgun sequence.
ACCESSION   DATDMW010000304 DATDMW010000000
VERSION     DATDMW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19712
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586407"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VI319_12770"
     CDS             complement(<1..315)
                     /locus_tag="VI319_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864762.1"
                     /translation="MKREARIVLALLLAGCRLPASAATAFENDYVKVSRNEAPCARDA
                     AGCRDRVILAMSPLELRSGGKSRKMDRGEVAIFKPGDSYEPPDAGAYYEVVVKPQHPP
                     VRC"
     gene            complement(312..803)
                     /locus_tag="VI319_12775"
     CDS             complement(312..803)
                     /locus_tag="VI319_12775"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEY6864763.1"
                     /translation="MRALSVVAVLALAACSANRPAETRDADRIVGTWKLVSVVYEDAV
                     TKERTPVLGEHPKGRQIATADGRWLALVTADGRKVPQTDAERAQALRTMIAYTGRYRV
                     VDGKVVTRVEAAWNEAWVGGEQVRFIRFEGDRLLHIESPPMPHPNVNGKVVRVIVTWE
                     RDL"
     gene            964..2598
                     /locus_tag="VI319_12780"
     CDS             964..2598
                     /locus_tag="VI319_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY6864764.1"
                     /translation="MERKTKRLVASAIALAVMAWFCGLEFRALFAPDEGRYADIAREM
                     LQSGDWITPRLNGLKYFEKPPLQYWATAVAFSLFGADEWSARLWPALTGFLGMVFAAF
                     AGARLAPRTRPFDIALMFAGCWAYFLGAQFLTLDMGLTFFLTVAMLAFLLSRAPGAAP
                     AAERGWMLAAWTAMALAVLSKGLIGIVLPALALLAYIAIERDWSLLRRLRWGGGLALF
                     SLITVPWFVLVQQRNPEFLRFFFVYEHFERYLLPDHHRPGPWWYFVPVLLLGLLPWTA
                     RLPGAIARGWTAPAAGGLRVDRFLVIWAAVITVFFSASESKLPGYVLPAVPALLLLIA
                     RNAPGVRDPSAATSLLAGAGLLALAALVPWLHAPGALTAMPAAYSAWLLAAGSVLCAA
                     ALAALALRRRADAPTVAVVGMGSLLALQLALSGMHVLDADFSSERLIERVAGEDKIFP
                     RDPPFYSVATFDHSVPFYLGRPVTLVANKNELGPGIAAEPGKYVRSIDEFVGRWSRDA
                     EAFAIMSPSVYEKLHGQGLPCRVVVRDERRVLISRR"
     gene            complement(2604..4748)
                     /gene="glgX"
                     /locus_tag="VI319_12785"
     CDS             complement(2604..4748)
                     /gene="glgX"
                     /locus_tag="VI319_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY6864765.1"
                     /translation="MTVPSALKSRLREGLPFPRGATWDGRGVNFSLFSANATKVEVCI
                     FDETGTKERERIELPEFTSEIWHGWVPDLRPGTVYGYRVHGPYAPDEGHRFNPNKLLL
                     DPYARAHIGRLEWDPAIFGYTVGHADADLSFDERDSAPFVPKGVVVDPNFDWARENVR
                     KRVLWDQAVFYELHVRGYTKLHPAVPESERGTFAGLANPAVIDYVRSLGVTSIELLPV
                     HAFVNDSYLLDKGLTNYWGYNTIGFFAPDPRYSANPARALQEFKEMVARFHDAGLEVI
                     LDVVYNHTAEGNELGPTLSFKGIDNASYYRLIPDRKRYYINDTGTGNTLNPSHPRVIQ
                     MITDSLRYWVQEMHVDGFRFDLGTILAREPHGFDEQSPFLMACTQDPVLSTVKLIAEP
                     WDCGPGGYQVGRFQPGWAEWNDRFRDTVRDYWRSEASAAALAPRLCASPDLFNHRGRK
                     PSASVNFVTAHDGFTLNDLVSYNDKHNEANGENNQDGSSNNRSWNCGAEGASDDPEVL
                     KLRERQKRNFLATLLLSQGTPMMVAGDEFGRTQNGNNNAYCQDNEISWVGWDYGERGA
                     ALAAFVQTLTALRRRFPILRFTRFLSGTLNEELGVKDVTWISATGAEMKTEEWEDGNT
                     RCFGMLLDGRAQPSGIRQRGQETTLLAVFNAWHDVVNFTLPESNGGGGWSLLLDTNIP
                     HEKEAAAFAFGHAYAVTGRSLLLFELVESRAR"
     gene            complement(4921..5352)
                     /locus_tag="VI319_12790"
                     /pseudo
     CDS             complement(4921..5352)
                     /locus_tag="VI319_12790"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
     gene            complement(5369..6088)
                     /locus_tag="VI319_12795"
     CDS             complement(5369..6088)
                     /locus_tag="VI319_12795"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEY6864766.1"
                     /translation="MRAGEPQIVAIGGGGLSADPDRYAIERYIVGNARRARPSVCYVG
                     TASGDENAYAVRFYAAMAELDCRPSRLPFFARTPDLRKLVLAQDVVYVGGGNTRSMLA
                     VWREWGLDQVLREAWENGTLLCGTSAGAICWFESGVTDSWADALQPMPCLGFLPGACC
                     PHYDGEKERRPAVQRLVSEGRLASVLALDDGAAAHFRGTALAGIVTSRPKALGYIVKC
                     GVEKSGSAAREEPLPARYLID"
     gene            6146..6760
                     /locus_tag="VI319_12800"
     CDS             6146..6760
                     /locus_tag="VI319_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEY6864767.1"
                     /translation="MDRLDPRRTALVLFDILNGHVKTRDAEARWRPVIAAAATLLAAA
                     RERGVMVAYACAAHRADNATSAHTVRDTDNRLRPLDPGAPPSFTPVVAAGSWGAQIVD
                     ELAPEPQDYVVPKYRWSAFHQTYLDLALRTRGIDAVVLAGGSTDVGVASTAYAARDLD
                     YNVVIAADACTSPERDNHEQFMKRVFPRMARVRTAAQVVAMLSA"
     gene            6952..7158
                     /locus_tag="VI319_12805"
     CDS             6952..7158
                     /locus_tag="VI319_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY6864768.1"
                     /translation="MSTGTVKWFNDSKGFGFITPDDGGKDLFAHFSAIQGSGFKTLKE
                     NQKVTFDVAQGPKGPQAQNIRPIE"
     gene            7333..7740
                     /locus_tag="VI319_12810"
     CDS             7333..7740
                     /locus_tag="VI319_12810"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEY6864769.1"
                     /translation="MKGFAAAASVLIGCATLSGCETLALSAIGASASAGLSYTMNGAA
                     YRTFTAPENKVRAAAFTALKRMASPIDSTEKTDEGELIKASMPQRSIEVHIEPLSPNA
                     TRVRAVAKRGAFLHDTATAAEVISQTERALSLR"
     gene            complement(7742..9601)
                     /locus_tag="VI319_12815"
     CDS             complement(7742..9601)
                     /locus_tag="VI319_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6864770.1"
                     /translation="MKSLLKQVMLKYRKELKLGAALVALAGSFLLVWLTITAPDRGRD
                     PRVTAMEKESDAFFRVEGGITGLAEDLKLGSVASIGLSPNYALVGLNDGSRYYVRIDA
                     QKTLLPEVIKASLSGPSVPVFSLGDVQPPSGPLTAFAHALADPVWVSLLGPLLILYMI
                     WMMNPVRFGMFKNAQKPDTRFADVIGVEDAKEALEDIVAYLRNPGKFSDLGAKPPKGV
                     VLEGHFGAGKTLLARAVAGEAGVPFIALAGGDFSDMFLGVGVRRVKKLFALARRQAPC
                     VIFIDEIDGLGRRSGASSAAETENNRIINTLLVELDGFAANSGIVVLGATNNVQNLDP
                     ALIRPGRFDRTCTVGLPSVAEREALFDLYARRVRTDGRTDYRQLARLSTGLSPASIAN
                     IVNAAALAAAKEEADAVTQDHFHRALEQHLMGGPVAAGQAAMNAAERERIAVHEAGHA
                     LVAKLMQVGIVEKVSILKRGRALGVTLVTHDQDITLQSEAQVRARMAMLLGGRCAEAL
                     VLGSISTGAANDLEHVGNMAYRMVTEFGFSRNIGPFSYAGLPDKERRLLDDPDTIAEA
                     RAIAKDIERECTELLSSQRGALERLSAELLEHETVDGGVVDACLETAPMALAA"
     gene            9895..11292
                     /locus_tag="VI319_12820"
     CDS             9895..11292
                     /locus_tag="VI319_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6864771.1"
                     /translation="MKRLLAIAVLCACAVAPCAPGRAADDEDGAPVDWIGVPGASAER
                     VDEPVWGGKVMVYRVGRRDADPVVLVHGIGQDGARDWRYVIQELARDHDVWALDLPGF
                     GESDKGNNLYSPERYVRVLEAVVGRRVGRPFVLIGHSMGAAVSLEYAATYPKRVARLI
                     LVDMAGVLHRAIFAESMGRLGVEQATGFYPEEGSWVGSVMRRMLAKVEPLAGASAAVL
                     RTAETRQRFLNGDPGAIAGYALVEHDFGRALRAVKAPTLLVWGSEDRIAPMRTAQVAL
                     ATIPGARLRIIQGVRHEPMTQVPQRFNEIVRDELDGRAGGVQYESPAGAFKSDRVGRC
                     KGVRGRGFSGDYERIELDRCPEAELTGARVGTLVVTDSTVHVLDSQIRDGVQAMRSRV
                     DITGGVAGGSPPFVMDTSTVDVAGARIEAEDAVARNIGRERSTLRFSVSEVSRDGGTP
                     SPVHRVVRIDPDKDW"
     gene            11343..12203
                     /locus_tag="VI319_12825"
     CDS             11343..12203
                     /locus_tag="VI319_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6864772.1"
                     /translation="MAYCDWGDAHDRHVVMCVHGLSRNARDFDYLAQALRRKCRVVCM
                     DVVGRGDSEWLEDKSGYSFRTYLYDAAALIARVTTPVPPGMFRLGGRTTKLDWVGTSM
                     GGLIGLLLAARPHSPIRRLVLNDVGPFVPWDALFRLKGQAPDGASFKSLADAEAHLRE
                     ACASFGIRSREHWRHLVRHSVRNTKDGRWALRYDPAIGRPRSQWDRELPLGAEFMRGI
                     DVWPAWGEVRCPVLVLRGAESDVLTAATVAEMRRRKPDVQVVEFPGVGHAPALFDPDQ
                     IAVVRRFLMR"
     gene            complement(12208..12618)
                     /locus_tag="VI319_12830"
     CDS             complement(12208..12618)
                     /locus_tag="VI319_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6864773.1"
                     /translation="MTNPELAGTAALLLRISLAILFFAHAWLKIKVFTPAGTVKYFAS
                     LGVPGWFAYATVAAEIGGGALLLLGIETRWVALLLVPLLLGTIVLVHGKNGWQFTNSG
                     GGWEYPAFWIVGLLALALLGDGSAALGPMMGLPG"
     gene            13126..13914
                     /locus_tag="VI319_12835"
     CDS             13126..13914
                     /locus_tag="VI319_12835"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY6864774.1"
                     /translation="MAGKVVPVEGDALHYDGAGALRSLRAGDRLYEGDRLITGADGDV
                     HVALADGGYMAVRPGSELRVDVFKADGGRDDRSEISLAKGSFRTVTGWIGAAALIRTL
                     DATIRVRGTDHEPLVVPEAALRGEPGTYDRVNAGAVVIDTPQGRLEVRAGEAGFAPRG
                     LGLPPEPLRVIPTLYAPTRNEGVFAGLNERIKSQVERLRDDRRSAGADRAGGPERADR
                     PERADRPDRPDRPDRPDRPDRPDKPDRPDKPDRPDKPDKPGRNR"
     gene            13964..14317
                     /locus_tag="VI319_12840"
     CDS             13964..14317
                     /locus_tag="VI319_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6864775.1"
                     /translation="MKRLVLFLLSLGCLCANAALAQDVPALDDTQLLGMRLFNQSCRV
                     CHTPPHAGSPQYGPVLSRDSLGGQDETLRSFIASGSPRMPGFRSQFRPEEIAALAAYL
                     KTLPPSEPARRPESE"
     gene            14326..16161
                     /locus_tag="VI319_12845"
     CDS             14326..16161
                     /locus_tag="VI319_12845"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY6864776.1"
                     /translation="MRTLAALALLCVVGSAHAADALLSGAVRSAQGEKMGGVTVSAKP
                     EGGTITTTVFTDESGEYFFPALASGKYRVWAQALGYRTSRSPVDLSSSAKQDFTLARM
                     TTAEAVYRQLPGHLMLASLPEDTPQDRRMKRLVRNDCTGCHTPSYPLQHRFDEAGWNA
                     IIELMKNVNVGGTYVGKERKPAGLLDFHQKELAAYLARARGPQQSYANVRLGARPAGE
                     PARAVFREYDLPLDPDAGLPANFVQNDGSDWSLGTPSVLIPGWGVHDAWLDLSGDIWF
                     TCNLPNKRTTIGRIDSRSGDVKLVRIPGRGELAAQTHGMTRAPDGTIWFNVNPGRGGL
                     AHLDPKTEKIDVYMPPEGMSPTGGATTVDWDGKGRIWVSAPDGALRFDPQTQSFTGYK
                     SLTYKTPNGTGTTYGAAADRNGNGYWAEMQLDIIGVGDGASTGPVGQAAEIRLAPVKE
                     EMDRVAPEARKFYETYAQPDFNGPFPWAQGPRRMGTDKAGDVLWVGDSWGGTLARIDT
                     VTRAVSYVKLPGDLQPYQVQVDSQHRPWTNLWGADRLMRYDPATGKWDAFELPTRGAE
                     PRYLSLDERDGTMRVVIPYYRARKVAVMTTRTEAELRELKARAAR"
     gene            complement(16158..17300)
                     /locus_tag="VI319_12850"
     CDS             complement(16158..17300)
                     /locus_tag="VI319_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864777.1"
                     /translation="MATKTRIAAAALAAGFALAAQAQDILIGQVSSQTSSVTAANAKA
                     LYAGITAYFEYVNAKGGVDGRKVKLVNKDDELKTDKMIALTHELIADRNVLALAGYQN
                     TGGITEVAKQDIAGKSGIAMIAPFQGDKSIVSAPNFFPFRSGYPDEVIALMREAVFTQ
                     KKRVVIVAQNVTYGPPIMKLIQEEAPKEGLSVAAYVSLDATQPEKIPAIVKDVVAQVV
                     KLNPDAVLILGGGRHTFEFVKQFKESPAESAQLYLMSVVPPESIVKMVGEQKARGIVI
                     SQAVPYPYSATLPLVGEYQKLMKQYAPNEPLSFSSLEGFVVAKITVEALKRASPKPTR
                     EKVLKAVAHMGELNLGGVYVNYTPKERKGWGGVDLTVIGPGGKLLR"
     gene            complement(17433..17846)
                     /locus_tag="VI319_12855"
     CDS             complement(17433..17846)
                     /locus_tag="VI319_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864778.1"
                     /translation="MCAAGPARADPAEELERLRAESARLHLEVDALDARIRALERGVG
                     NGAVRADGPAAAAAPLRPDLSPLAQLRLSWSRVERGVPAERVESLLGKPDKLLRIDGS
                     PVWYYVYPGVGRGSVFFDAGGKVSSTQSPDAGWSR"
     gene            complement(17891..18505)
                     /locus_tag="VI319_12860"
     CDS             complement(17891..18505)
                     /locus_tag="VI319_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009907921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6864779.1"
                     /translation="MRIKIPVSASIVALACLAAPAGAQDADAAKIATQTCAACHGARG
                     ESVSPAFPRLAAQRVEYLDAQLKAFRDRTRADPMAQAYMWGMASQLDDATIRGLADYY
                     SRQKAVHSAPARTALAQAGRDIFEKGVPAAQVQACATCHGKDAQGTSAFPRLAGQHAE
                     YIVKQLVLFKEQLREEGTAPVMHGVSRGMSFEQMEAVAAYLASR"
     gene            complement(18519..>19712)
                     /locus_tag="VI319_12865"
     CDS             complement(18519..>19712)
                     /locus_tag="VI319_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEY6864780.1"
                     /translation="LPQVQGISQSKEDELLLNQVPPFSFMWQTSYTRTSKAVPDSGAG
                     IDGNSQNGQVLFPQQASLFYAGRVAPGLGSFVQITYDSVAGNVHWDNTELRYAQQASL
                     LSSPLTWGLTMNNNPTVQDIFNSTPAWQTPFDQRSNAAPVPGAATLIDGGLAGRVAGL
                     TGYLWWKNSVYAELGLYRSAPQGHEFRNGAGPLDSTNADGTLISNAPYWRVAYEHQWD
                     RNSLSVGAYGLTAKISPEGQPVGPPADKFVDTAVDAQYQFIGDEHILSGQTTYIKEKQ
                     TLDATFGAAGSDNATNDLKTFRVGASYYYRRNYGGALGYFSTTGSADATLRGMAAVTG
                     FANGSPNSNGWIGELDWVPYQNVKVALQYIAYDKFNGAKTDYDGSGRNAKDNNTLYLL
                     GWLAF"
BASE COUNT         2794 a         6792 c         6984 g         3142 t
ORIGIN      
        1 gcatctcacc ggcggatgct gcggcttcac gaccacctcg tagtacgcgc cggcgtcggg
       61 cggctcgtag ctgtcgcccg gcttgaagat cgccacctcg ccgcggtcca tcttgcggga
      121 cttgccgccg gagcgcagct cgagcggaga catggcgagg atcacgcggt cgcggcaccc
      181 cgcggcgtcg cgcgcgcacg gcgcctcgtt gcggctgacc ttgacgtagt cgttctcgaa
      241 cgccgtcgcg gcgctcgccg gcagccggca tccggcgagc agcaaggcga ggacgatgcg
      301 ggcttcgcgc ttcacaggtc tcgctcccag gtgacgatga cgcgcaccac cttgccgttc
      361 acgttcgggt gcggcatggg cgggctctcg atgtgcagca ggcggtcgcc ctcgaagcgg
      421 atgaagcgca cctgctcgcc gccgacccag gcctcgttcc acgcggcctc gacgcgcgtg
      481 acgaccttgc cgtccacgac gcggtagcgg ccggtgtacg cgatcatcgt gcgcagcgcc
      541 tgcgcgcgct cggcgtcggt ctgcggcacc ttgcggccgt cggccgtcac gagcgcgagc
      601 cagcgcccgt ccgcggtcgc gatctgccgg cccttcgggt gctcgccgag gacgggcgtg
      661 cgctccttcg tgaccgcgtc ctcgtagacg accgagacga gcttccaggt gccgacgata
      721 cggtcggcgt cgcgggtctc ggctggacgg ttggcggagc aggcggcgag cgccagcacg
      781 gcgacgacgg acaatgcgcg cacgaggcct cccgggaagg ggttttccgc cgcctatgat
      841 aggggaagac cgcagcggcc gcagtcgccc gacgacagct tgcggcaagg caatcgtaag
      901 ccccgagtca ggaccgcttc ccataatcgg cgtcctcgcg tccgaacccg tcgcggaggc
      961 ccgttggaac gcaagacgaa gcgcctggtc gcgtccgcga tcgcgctggc tgtgatggcc
     1021 tggttctgcg gcctggaatt ccgcgcgctg ttcgccccgg acgagggccg ctacgccgac
     1081 atcgcgcgcg agatgctcca gtcgggcgac tggatcacgc cgcggctgaa cggcctgaag
     1141 tatttcgaga agccgccgct ccagtactgg gcgaccgccg tcgcgttctc cctgttcgga
     1201 gcggacgagt ggagcgcgcg cctgtggccg gccctcacgg gcttcctcgg catggtcttc
     1261 gccgcattcg ccggcgctcg cctggcgccg cgcacgcgtc cgttcgatat tgcgctcatg
     1321 ttcgcgggct gctgggcgta tttcctgggc gcccagttcc tcacgctcga catggggctc
     1381 acgttcttct tgacggtggc gatgctcgcg ttcctgctga gccgggcgcc tggcgcggcg
     1441 ccggccgccg agcggggttg gatgctcgcc gcgtggaccg cgatggcgct cgccgtgctg
     1501 agcaagggcc tgatcggcat cgtgctgccc gcgctcgcgc tgctcgcgta catcgcgatc
     1561 gagcgcgact ggtcgctgct gcgccggctg cgctgggggg gcgggctcgc gctcttctcg
     1621 ctgatcacgg tgccgtggtt cgtgctggtc cagcagcgca atcccgagtt cctgcggttc
     1681 ttcttcgtct acgagcattt cgagcgctat ctgctgcccg atcatcatcg gcccgggccg
     1741 tggtggtact tcgtgccggt gctgctgctc gggctgctcc catggacggc tcggctgccc
     1801 ggcgctatcg cgcgcggatg gacggcgccc gcggccggcg gtctgcgggt cgaccgcttc
     1861 ctcgtgatct gggcggccgt catcacggtc ttcttcagcg cgtcggaatc gaagcttccc
     1921 ggctacgtgc ttcccgccgt tccagctctg ctgctgctga tcgcgcgcaa tgcgccgggc
     1981 gtgcgcgatc cttcggcggc gacgtcgctg ctcgcggggg ccgggctgct cgcgctcgcc
     2041 gcgctggtgc cctggctgca cgcccccggc gcgctcaccg cgatgccggc cgcttattcg
     2101 gcgtggctgc tcgccgccgg gtcggtgctg tgcgcggccg cgctcgcggc gctcgcgctg
     2161 cggcgccgcg cggacgcgcc caccgtcgcc gtcgtgggca tgggcagcct gctcgcgctg
     2221 cagctcgcgc tttcggggat gcacgtcctc gacgcggatt tctcctcgga gcggctgatc
     2281 gagcgggtcg ccggggagga caagatcttt ccccgcgatc cgcccttcta cagcgtggcg
     2341 acgttcgacc acagcgtgcc cttctatctc gggcggccgg tgacgctggt cgcgaacaag
     2401 aacgagctcg gtcccggcat cgcggccgag cccggcaagt acgtgcgctc catcgacgag
     2461 ttcgtcgggc gctggagccg cgacgccgag gccttcgcga tcatgtcgcc gagcgtgtac
     2521 gagaagctgc acggccaggg cctgccctgc cgcgtcgtcg tgcgcgacga gcgccgggtg
     2581 ctgatctcgc gccgctagcg cgatcagcgc gcgcggctct cgaccagctc gaacagcagg
     2641 agcgagcgtc cggtcaccgc gtaggcgtgc ccgaacgcga acgccgcagc ttccttctcg
     2701 tgcgggatgt tcgtgtcgag cagcagcgac cagccgccgc cgccgttgct ttccggcagg
     2761 gtgaaattca ccacgtcgtg ccaggcgttg aagacggcca gcagcgtcgt ctcctggccg
     2821 cgctggcgga tgccgctggg ctgcgcgcgg ccgtcgagca gcatgccgaa gcagcgcgtg
     2881 ttgccgtcct cccattcctc ggtcttcatc tcggcgccgg tggcgctgat ccaggtgacg
     2941 tccttgacgc cgagctcctc gttcagcgta ccggagagga accgcgtgaa gcgcaggatc
     3001 gggaagcggc gccgcagcgc cgtcagcgtc tgcacgaagg cggcgagcgc ggcgccgcgc
     3061 tcgccgtaat cccagccgac ccagctgatc tcgttgtcct ggcaataggc gttgttgttt
     3121 ccattctgcg tgcggccgaa ctcgtcgcct gcgaccatca tcggcgttcc ctgcgagagc
     3181 agcagggtgg cgagaaagtt gcgcttctgg cgctcgcgca gcttgaggac ttcgggatcg
     3241 tcgctcgcgc cctcggcgcc gcagttccag gatcggttgt tggagctgcc gtcctggttg
     3301 ttctctccgt tcgcctcgtt gtgcttgtcg ttgtagctca ccaggtcgtt cagcgtgaag
     3361 ccgtcgtggg cggtgacgaa gttgacgctc gccgacggct tgcggccgcg gtggttgaag
     3421 aggtcgggcg acgcgcacag cctgggtgcg agcgccgcgg cgctcgcctc gctcctccag
     3481 taatcgcgca cggtgtcgcg gaacctgtcg ttccactcgg cccagcccgg ctggaagcgc
     3541 ccgacctggt agcctccggg tccgcagtcc cagggctcgg cgatcagctt cacggtcgag
     3601 agcaccgggt cctgcgtgca cgccatcagg aacgggctct gctcgtcgaa gccgtgcggc
     3661 tcgcgcgcga ggatggtgcc gaggtcgaag cggaagccgt cgacgtgcat ctcctgcacc
     3721 cagtagcgca ggctgtcggt gatcatctgg atcacgcgcg ggtggctcgg attgagcgtg
     3781 ttgcccgtgc ccgtgtcgtt gatgtagtag cgcttcctgt ccggaatgag gcggtagtac
     3841 gaggcgttgt cgatgccctt gaacgagagc gtcggcccga gctcgttgcc ttccgcggtg
     3901 tggttgtaga cgacgtcgag gatcacctcg agccccgcgt cgtggaagcg cgccaccatc
     3961 tccttgaact cctgcagcgc gcgcgccggg ttcgccgagt agcgcgggtc gggcgcgaag
     4021 aagccgatcg tgttgtagcc ccagtagttg gtgagcccct tgtcgaggag gtacgagtcg
     4081 ttcacgaagg cgtgcaccgg cagcagctcg atcgaggtga cgccgagcga ccggacgtaa
     4141 tcgattacgg cagggttcgc caggccggcg aaggtcccgc gctcggactc gggcaccgcg
     4201 ggatggagct tcgtgtagcc gcgcacatgc agctcgtaga aaacggcctg gtcccaaagc
     4261 acgcgcttcc ggacgttctc gcgcgcccag tcgaagttcg ggtcgaccac cacgcccttc
     4321 ggcacgaagg gcgcgctgtc gcgctcgtcg aacgacaggt cggcgtccgc gtgcccgacg
     4381 gtgtagccga agatcgccgg gtcccactcg agcctgccga tgtgcgcgcg cgcgtacggg
     4441 tcgagcagca gcttgttcgg gttgaagcgg tgaccctcgt cgggcgcgta cggcccgtgc
     4501 acgcggtagc cgtacaccgt gccgggccgc aggtcgggca cccagccgtg ccagatctcg
     4561 ctcgtgaact cgggcagctc gatgcgctcg cgctctttcg tcccggtctc gtcgaagatg
     4621 cagacttcga ccttggtggc gttggccgag aacagcgaga agttgacgcc cctgccgtcc
     4681 caggtcgcgc cgcgcgggaa gggcaggccc tcgcgcaggc ggctcttgag ggcggaggga
     4741 acggtcatgt ccatgtcggg ctcccggggc agcgtgtcgt gggaaaaaga cagaccacgc
     4801 tactccggtt ttgtgcccgg aatcgcggcg cgaacccggc cgatcaggct ccttcgccgc
     4861 ggcctcccgc aggtacaccg gcaggtcggt gaggggcggc cgcgccggca cctggtagaa
     4921 catgtcggcg acgaagccgc ggtggtagct cgtgtggttc accacgtgca gcatgacgtc
     4981 ggcgcgcttc atctcgccgc ggttgccgct caccagcttg aagcgcacgg tctcgtcggc
     5041 ctgcgccgcg gtcagctcgt cggcccagcc gatgtaccag tcgtcgatgt cctgctgcac
     5101 ctcccacagc tcggggagat cgtcgtgcag cacggtgttc agcgccggga tgccgtggtc
     5161 gcgtccctgc aggtgcgcct gccagatgag gtcgacgaca tagagatggt tgagcgtgtg
     5221 caccatgctc gtgaagagcg actggcgctc ccgggtcgcc tcgcccgcgg gcagcgcggc
     5281 gacggcctcg aacagtgtct tgttcgccca ggcgttgtag cgggcgagca tgcgggtggt
     5341 cttgatgtcc atggtgagct ccggtgaatc aatcgatcag gtaccgggcg ggcaggggct
     5401 cctcgcgcgc ggcgctgccg ctcttctcga cgccgcattt cacgatatat ccgagcgcct
     5461 tcggccgaga cgtcacgata cccgcgagcg ccgtgccgcg gaagtgcgcg gccgcgccgt
     5521 cgtccagcgc gagcaccgag gcgagccggc cctcggagac gaggcgctgc acggcgggcc
     5581 ggcgctcctt ctccccgtcg tagtgcgggc agcaggcgcc gggcaggaat ccgaggcacg
     5641 gcatgggttg cagcgcgtcg gcccaggaat cggtgacgcc cgactcgaac cagcagatcg
     5701 cgccggcgct ggtcccgcac aggagggttc cgttctccca ggcctcgcgc agcacctggt
     5761 cgagacccca ctcgcgccac accgccagca tgctcctcgt gttgccgccg ccgacgtaca
     5821 cgacgtcctg cgcgagcacg agcttgcgca gatcgggcgt gcgcgcgaag aacggcagcc
     5881 gcgacggccg gcagtcgagc tcggccatcg ccgcgtagaa gcgcaccgcg tacgcgttct
     5941 cgtcgccgct cgcggtgccg acatagcaca ccgacggccg tgcgcggcgc gcgttgccca
     6001 cgatgtagcg ctcgatcgcg tagcggtcgg gatcggccga gaggccgccg ccgccgatgg
     6061 cgacgatctg gggctcgcct gcgcgcatcg tccaaggtta ccgcgccgcg cgcgcgggcg
     6121 tccacgccgc ggtaagcttc gcgtcatgga ccgtctcgat ccgcggcgca cggcgctcgt
     6181 cctgttcgac atcctgaacg gccacgtgaa gacgcgcgac gccgaggcgc gctggcgccc
     6241 cgtgatcgcg gcggcggcga cgctgctcgc ggccgcgcgc gagcgcgggg tgatggtcgc
     6301 gtacgcctgc gccgcgcacc gggccgacaa cgcgaccagc gctcacaccg tgcgcgacac
     6361 cgacaaccgg ctgcggccgc tcgatcccgg cgcgccgccc tcgttcaccc cggtcgtggc
     6421 cgcgggcagc tggggcgcgc agatcgtcga cgagctcgcg cccgagccgc aggactacgt
     6481 ggtgccgaag taccgctgga gcgctttcca ccagacctac ctggacctcg cgctgcgcac
     6541 ccgcggcatc gacgcggtcg tcctcgcggg cgggtcgacc gatgtcggcg tcgcgagcac
     6601 ggcctacgcc gcccgcgacc tcgactacaa cgtcgtcatc gccgccgacg cctgcacctc
     6661 gcccgagcgc gataaccacg agcagttcat gaagcgggtg tttcccagga tggcgcgggt
     6721 ccggaccgcg gcgcaggtcg tcgccatgct gtcggcatag gcgcgggttt cagaaagtgc
     6781 ttgacctgcg gcacttctcc ctatataaga cggcctgcgg tactcaaagc ttcgaagtca
     6841 ttagtccatt gcagccgctg atagcggatc caccaaggcc ttaacttctt tcttgcagtg
     6901 tccgcgaacg ggcgcaagcc ttcatataca taattttttg ggaagacaga tatgagcaca
     6961 ggtaccgtca agtggttcaa cgactccaag ggcttcggtt tcatcacgcc cgatgacggc
     7021 ggcaaggacc ttttcgcgca cttctcggcg atccagggca gcggcttcaa gacgctcaag
     7081 gagaaccaga aggtgacctt cgacgtcgct cagggcccca agggcccgca ggcgcagaac
     7141 atccggccga tcgagtaact ccttccggtt tccggacaga aaagcccggc ctcgagccgg
     7201 gcttttttcg cccgcttttc gcagcacggc gcgaggcaga aatccacgcg cggggagacg
     7261 cgggcattgc ttgcccgtct cgccggcggc tagattggac cgcgccgcag tcccgcggcg
     7321 aggaggggtt cgatgaaagg cttcgccgcc gcagcgtccg tcctgatcgg gtgcgcgacg
     7381 ctttccggct gcgagaccct cgccctgtcg gccatcggcg cgagcgcctc ggccggcctg
     7441 tcctacacca tgaacggggc ggcgtaccgc acgttcaccg cgccggagaa caaggtccgc
     7501 gcggccgcct tcaccgcgct caagcgcatg gcgagcccca tcgattcgac cgagaagacc
     7561 gacgagggcg aattgatcaa ggcgtcgatg ccgcaacgga gcatcgaggt ccacatcgag
     7621 cccctcagcc cgaacgccac gcgcgtgcgc gccgtggcca agcgcggcgc cttcctgcac
     7681 gacaccgcga ccgccgccga ggtgatcagc cagaccgagc gcgcgctcag cctgaggtag
     7741 ctcaggcggc gagcgccatc ggcgcggtct ccaggcaggc gtctaccacc ccgccgtcca
     7801 cggtctcgtg ctcgagcagc tccgccgaca ggcgttcgag cgcgccgcgc tgcgacgaca
     7861 gcagctccgt gcactcgcgc tcgatatcct tggcgatggc gcgcgcctcg gcgatcgtgt
     7921 ccgggtcgtc gagcaggcgc cgctccttgt cgggcagccc cgcgtagctg aacggcccga
     7981 tgttccgcga gaagccgaac tcggtgacca tgcggtaggc catgttcccg acgtgctcga
     8041 ggtcgttcgc cgcgccggtc gagatcgagc cgagcacgag cgcctcggcg cagcgaccgc
     8101 cgagcagcat cgccatgcgg gcgcgcacct gcgcctcgct ctgcagggtg atgtcctggt
     8161 cgtgcgtgac gagcgtgacg ccgagggcgc ggccgcgctt gaggatactg accttctcga
     8221 cgatgccgac ctgcatcagc ttcgcgacca gcgcgtggcc ggcctcgtgc acggcgatgc
     8281 gctcgcgctc ggccgcgttc atcgcggcct gtcccgcggc gaccggcccg cccatcaggt
     8341 gctgctcgag cgcgcggtgg aaatggtcct gggtcacggc gtcggcctct tccttggcgg
     8401 cggcgagcgc cgcggcgttg acgatattcg cgatcgacgc gggcgagaga ccggtcgaga
     8461 gacgggcgag ctgccggtaa tcggtccggc cgtcggtacg cacgcgacgc gcgtacaggt
     8521 cgaacagcgc ctcgcgctcg gcgacgctcg gcaggcccac ggtgcaggta cggtcgaagc
     8581 ggcccgggcg gatcagcgcc gggtcgaggt tctgcacgtt gttggtcgcg ccgagcacga
     8641 cgatgcccga gttcgcggcg aagccgtcga gctcgaccag cagcgtgttg atgatgcggt
     8701 tgttctcggt ctccgctgcg gacgacgcgc ccgagcgccg gcccaggccg tcgatctcgt
     8761 cgatgaagat cacgcacggc gcctggcggc gcgcgagcgc aaacagcttc ttcacgcggc
     8821 gcacgccgac gccgaggaac atgtcgctga agtcgccgcc cgcgagcgcg atgaagggca
     8881 cgcccgcctc gcccgcaacg gcgcgcgcga gcagcgtctt gccggcaccg aagtggccct
     8941 cgagcacgac gcccttcggg ggcttcgcgc ccagatccga gaacttcccc gggttcctca
     9001 ggtaggcgac gatgtcctcg agcgcctcct tcgcgtcctc gacgccgatc acgtcggcga
     9061 agcgcgtgtc gggcttctgc gcgttcttga acatgccgaa gcgcaccggg ttcatcatcc
     9121 agatcatgta caggatgagc agcggaccga gcagcgacac ccagacggga tcggcgagag
     9181 cgtgcgcgaa cgcggtcagc gggcccgacg gcggctgaac gtcgccgagc gagaacacgg
     9241 ggacggacgg cccggacagg cttgccttga tcacctcggg gagcagcgtc ttctgcgcgt
     9301 cgatgcgcac gtagtagcgg ctgccgtcgt tcaggccgac gagcgcgtag ttgggcgaga
     9361 ggccgatgct cgccaccgag ccgagcttga ggtcctcggc gaggccggtg atgccgccct
     9421 cgacgcggaa gaacgcgtcg gactccttct ccatcgcggt cacgcgcgga tcgcgcccgc
     9481 ggtcgggcgc cgtgatcgtg agccagacga ggaggaacga gccggcgagc gcgaccagcg
     9541 ccgcgccgag cttcagctct ttacggtatt tgagcatgac ctgcttgagc aaggacttca
     9601 cggcgttctc cctgatatgt cgttgttccg gacgttgccg gcgtgcgcga tcggcgcccg
     9661 cgtcgcgcgt gttttgccag tcgggggttt gcggtgatgc gccgattgtg tcgttctgcg
     9721 gcgcgatccg ggtcttgttg gtcccgttgc ggcggccgcc ttcgcgaccg ccgctgcctc
     9781 gcgatattgt atcagcgcgt cgcggacttt cggccgcgtt tcgatcagcc attgtgattc
     9841 cggcgacgcg gcgcttgatg gtccgaaggg actaattcaa tatactgagc gacgatgaag
     9901 cgactgctcg caatcgccgt gctctgcgcg tgcgccgtcg cgccttgcgc gccggggcgc
     9961 gcggccgacg acgaggacgg cgcgcccgtc gactggatcg gcgtcccggg cgcgagcgcc
    10021 gagcgcgtcg acgagccggt gtggggcggc aaggtgatgg tctaccgcgt cgggcgccgc
    10081 gacgccgatc cggtggtgct cgtgcacggc atcggccagg acggcgcgcg cgactggcgc
    10141 tacgtcatcc aggagctcgc gcgcgaccac gacgtctggg cgctcgacct gcccggcttc
    10201 ggcgaatcgg acaagggcaa caatctctat tcgccggaac gctacgtgcg cgtgctcgag
    10261 gccgtggtcg gccggcgcgt cggccgcccc ttcgtcctga tcgggcactc gatgggcgcc
    10321 gccgtctcgc tcgagtacgc ggcgacctac ccgaagcgcg tggcgcggct gatcctggtc
    10381 gacatggcgg gcgtgctgca ccgggcgatc ttcgccgagt cgatgggacg cctcggcgtc
    10441 gagcaggcga ccgggttcta tcccgaggag ggctcgtggg tcggctcggt gatgcggcgc
    10501 atgctcgcga aggtcgagcc gctcgccggc gcgagcgccg ccgtcctgcg cacggccgag
    10561 acgcggcagc ggttcctgaa cggcgatccc ggcgcgatcg ccggctacgc gctggtcgag
    10621 cacgacttcg gccgcgcgct gcgcgcggtc aaggccccga ccctgctcgt gtggggcagc
    10681 gaggaccgga tcgcgccgat gcggacggcg caggtcgcgc tcgcgacgat ccccggcgcg
    10741 cggctcagga tcatccaggg cgtgcgccac gagcccatga cgcaggtgcc gcagcgcttc
    10801 aacgagatcg tgcgcgacga gctcgacggg cgcgcgggcg gcgtgcagta cgagtcgccc
    10861 gcgggcgcgt tcaagagcga ccgcgtcggc cgctgcaagg gcgtgcgtgg gcgcggcttc
    10921 agcggcgact acgagcgcat cgagctcgac cgctgtcccg aggcggagct gaccggcgcg
    10981 cgcgtgggaa cgctcgtcgt caccgactcc acggtgcacg ttctcgattc gcagatccgc
    11041 gacggggtgc aggccatgcg ctcgcgcgtc gatatcacgg ggggcgtcgc gggcggcagc
    11101 ccgcccttcg tgatggacac ctcgacggtc gacgtcgcgg gcgcccgcat cgaggccgag
    11161 gacgcggtcg cgcgcaacat cggccgcgag cgctcgacgc tgcgcttctc ggtgagcgag
    11221 gtgtcgcgcg acggcgggac gccgagcccg gtgcatcgcg tcgtgcgcat cgacccggac
    11281 aaggactggt agccctgcgc gtcgagagct tctccagcct cggtcccgac ggcttccacc
    11341 gcatcgccta ctgcgactgg ggcgacgcgc acgaccgcca cgtcgtgatg tgcgtgcacg
    11401 ggctgtcgcg caacgcgcgc gacttcgact acctggcgca ggcgctgcgg cgcaagtgcc
    11461 gcgtcgtgtg catggacgtg gtcggccgcg gcgacagcga atggctcgag gacaagtcgg
    11521 gctacagctt ccgcacctat ctctacgacg ccgcggcgct gatcgcgcgc gtcaccacgc
    11581 ccgtgccgcc ggggatgttc cggctgggcg gccgcacgac gaagctcgac tgggtcggca
    11641 cctcgatggg cgggctgatc ggcctgctgc tcgcggcgcg tccccactcg ccgatccggc
    11701 ggctggtgct gaacgacgtg ggcccgttcg tgccgtggga cgcgctcttt cgtctcaagg
    11761 gccaggcgcc ggacggcgcg tcgttcaaga gcctcgccga cgcggaggcg cacctgcgcg
    11821 aggcgtgcgc gagcttcggc attcgctcgc gcgagcactg gcgccatctc gtgcgccaca
    11881 gcgtgcgcaa tacgaaggac ggccgctggg cgctgcgcta cgatccggcg atcggccgcc
    11941 cgcgatcgca gtgggaccgc gagctgccgc tcggcgcgga gttcatgcgc gggatcgacg
    12001 tctggcccgc gtggggcgag gtgcgctgtc cggtgctcgt gctgcgcggc gccgagtccg
    12061 acgtgctgac ggcggcgacc gtcgccgaga tgcgccggcg caagcccgac gtgcaggtgg
    12121 tcgagtttcc cggcgtcggc cacgcgccgg cgctgttcga tcccgaccag atcgcggtcg
    12181 tgcgccggtt cctgatgcgc tagcgcgcta cccgggcagg cccatcatcg gcccgagcgc
    12241 ggcggatccg tcgccgagca gcgcgagcgc gagcagcccc acgatccaga acgccgggta
    12301 ttcccaaccg ccgccgctgt tcgtgaactg ccagccgttc tttccgtgca cgagcacgat
    12361 ggtccccagg aggagcggca cgagcagcag tgccacccag cgcgtctcga tgccgaggag
    12421 cagcagcgcg ccgccgccga tctcggccgc cacggtcgcg taggcgaacc agccgggcac
    12481 gccgagcgag gcgaagtact tcaccgtgcc cgcgggcgtg aacaccttga tcttgagcca
    12541 ggcgtgggcg aagaacagga tcgcgagcga aatccgaagc agcagcgccg ccgttccagc
    12601 cagttccggg ttcgtcatgg tgttctcctc ggaggcggat cggacagctt agagcggaga
    12661 gcgacccggc tgcaactccc cgagatcgag gacgtccgcc gtgcgctcgc ccgcctcagg
    12721 cgcgcgatcc gcgagcgcta tccgaggata cggcgcatct tcgtcgacac cgcggcgatc
    12781 ggcgaatgag tgccgcccga gcggtcgctg cggtgcaaaa agacccgctt cggcgtgctt
    12841 actcggtctg cggagccgaa tccggcaaat agtcacaccc cgattgtttc agccgttgtc
    12901 gctcgtgccg cggggtgcga caaaggcgcg tcacggcctc tggggagggg cccggtcatt
    12961 ccaggaagaa tcggcgcgta aggcgcggaa accgggtaca gtgccgtccg ggaccacggt
    13021 cgggagggac cgttaatgcc ccgtctagga ttcttcgttg cggccttcgc gattgcagcg
    13081 caggccgcgc ttgcgcaggc cccggttccg gggccatcgc cgcgcgtggc gggcaaggtc
    13141 gtcccggtcg agggcgacgc gctccactac gacggagcgg gagccttgcg cagcctgcgc
    13201 gcaggcgacc gcctctacga gggcgaccgt ctcattacgg gtgcggacgg cgacgtccac
    13261 gtcgcgctgg ccgacggcgg ctacatggcc gtgcggcccg gcagcgagct ccgtgtcgac
    13321 gtattcaagg ccgatggcgg ccgcgacgac cgctccgaga tcagtctcgc gaaaggctcg
    13381 ttcaggacgg tgacgggctg gatcggcgcc gcagcgctga tccgcacgct cgacgccacc
    13441 atccgggtcc gcggcaccga tcacgagccg ctcgtcgtcc cggaggccgc gctgcgcggc
    13501 gagcccggca cctacgaccg ggtgaatgcc ggcgcggtcg tcatcgacac accgcagggc
    13561 aggctcgagg tccgcgccgg cgaggcggga ttcgcgccgc gcgggctcgg gctgccgccg
    13621 gagccgctgc gggtcattcc gactctttac gcgccgacgc gcaacgaggg cgtgttcgcg
    13681 ggcctgaacg agcgcatcaa gtcgcaggtg gaacggctgc gcgacgaccg ccgctccgcg
    13741 ggcgcggatc gcgcaggcgg gcccgagcgc gccgatcggc ccgagcgcgc cgatcgtccc
    13801 gatcgccccg accgccccga tcggccggac cgccccgatc ggccggacaa gcccgaccgg
    13861 cccgacaagc ccgaccggcc ggacaagccc gacaagcccg ggcgtaaccg ctagccccgc
    13921 gctcgcgggt gatacactcc gccggtccgt ccgtcggaga gccatgaagc gcctcgtcct
    13981 gttcctgctg tcgctcgggt gcctgtgcgc caacgcggcg ctcgcgcagg acgtgcccgc
    14041 actcgacgac acgcagctcc tcggcatgcg cctgttcaac cagtcgtgcc gcgtctgcca
    14101 cacgccgccg cacgccggca gcccgcagta cgggccggtg ctctctcgcg attccctggg
    14161 cggacaggat gagacgctgc gcagcttcat cgcgagcggg tccccgcgca tgccggggtt
    14221 caggagccag ttccggcccg aggagatcgc cgcgctcgcc gcctacctca agaccttgcc
    14281 cccttccgag ccggcgcgcc ggcccgaatc cgaataggag gtcccatgcg cacgctcgcc
    14341 gcgctcgccc tgctctgtgt cgtcgggtcg gcccacgccg ccgacgcgct cctctcgggc
    14401 gccgtgcgct cggcgcaggg cgagaagatg ggcggcgtga ccgtgtcggc caagcccgag
    14461 ggcggcacca tcacgacgac ggtgttcacc gacgagtcgg gcgagtattt ctttccggcg
    14521 ctcgcctcgg gcaagtaccg cgtgtgggcg caggcgctcg gctaccgcac gtcgcgcagc
    14581 ccggtggatc tctcctcgag cgcgaagcag gatttcacgc tcgcgcgcat gacgacggcc
    14641 gaggccgtgt accgacagct ccccgggcac ctgatgctcg cgtcgctgcc cgaggacacg
    14701 ccgcaggacc ggcgcatgaa gcgcctggtg cgcaacgact gcaccggctg ccacaccccg
    14761 agctacccgc tgcagcaccg cttcgacgag gcgggctgga acgccatcat cgagctgatg
    14821 aagaacgtga acgtcggcgg cacctacgtc ggcaaggagc gcaagcccgc ggggctgctc
    14881 gacttccatc agaaggagct cgccgcgtac ctcgcgcgcg cgcgcggccc gcagcagagc
    14941 tatgccaacg tgcgcctggg cgcgcgccct gccggcgagc cggctcgtgc cgtgttccgc
    15001 gagtacgatt tgccgctcga cccggacgcg gggctgccgg caaacttcgt gcagaacgac
    15061 ggcagcgact ggtcgctcgg cacgccctcg gtgctgatcc cgggctgggg cgtgcacgac
    15121 gcgtggctcg atctctcggg cgacatctgg ttcacctgca atctgccgaa caagcgcacg
    15181 accatcggcc gcatcgactc ccgctcgggc gacgtgaagc tggtcaggat cccgggacgc
    15241 ggcgagctcg ccgcgcagac gcacggcatg acgcgcgcgc ccgacggcac gatctggttc
    15301 aacgtcaacc cggggcgcgg gggcctcgcg cacctcgatc cgaagaccga gaagatcgac
    15361 gtgtacatgc cgcccgaggg catgtcgccc accggcggcg cgaccacggt ggactgggac
    15421 ggcaagggcc gcatctgggt gtcggcgccc gatggcgcgc tgcgcttcga cccgcagacg
    15481 cagagcttca ccggctacaa gtccctcacc tacaagacgc cgaacggcac cggcacgacc
    15541 tacggtgcgg ccgccgatcg caacggcaac ggctactggg ccgagatgca gctcgacatc
    15601 atcggggtcg gcgacggcgc gtcgaccggg cccgtcggcc aggccgccga aatccggctc
    15661 gcgccggtga aggaggagat ggaccgcgtc gcgcccgagg cgcgcaagtt ctacgaaacc
    15721 tacgcgcagc ccgacttcaa cgggcccttc ccctgggcgc aagggccgcg gcgcatgggc
    15781 accgacaagg cgggcgacgt gctctgggtg ggcgactcct ggggcgggac gctcgcgcgc
    15841 atcgatacgg tgacgcgcgc ggtgtcgtac gtgaagctgc ccggagatct ccagccctac
    15901 caggtgcagg tcgacagcca gcaccgcccg tggaccaacc tctggggcgc cgaccgcctc
    15961 atgcgctacg acccggcgac cggcaagtgg gacgcgttcg agctgccgac gcgcggcgcc
    16021 gagccgcgct atctgtcgct cgacgagcgc gacgggacga tgcgggtcgt gatcccgtac
    16081 taccgcgcgc gcaaggtcgc ggtgatgacg acgaggaccg aggccgagct gcgcgagctg
    16141 aaggcgcgcg ctgcgcgcta gcgcagcagc ttgccgccgg gtccgatcac cgtcaggtcg
    16201 acgccgcccc agcccttgcg ctccttcggc gtgtagttga cgtaaacgcc gccgagattg
    16261 agctcgccca tgtgggcgac cgccttgagc actttctcgc gcgtgggctt ggggctcgcg
    16321 cgcttgagcg cctcgacggt gatcttggcg acgacgaagc cctcgagcga ggagaacgag
    16381 agcggctcgt tgggcgcgta ctgcttcatg agcttctggt attcgccgac cagcggcagc
    16441 gtcgccgaat agggatacgg gacggcctgc gagatgacga tgccgcgcgc cttctgctcg
    16501 cccaccattt tgacgatcga ctcgggcggc acgaccgaca tgaggtagag ctgcgcgctc
    16561 tcggcgggcg actccttgaa ctgcttgacg aactcgaagg tgtggcggcc gccgccgagg
    16621 atgaggacgg cgtcgggatt cagcttcacc acctgggcga cgacgtcctt cacgatcgcc
    16681 gggatcttct cgggctgggt cgcgtcgagg ctgacgtagg cggcgacgct cagcccttcc
    16741 ttcggcgcct cctcctggat cagcttcatg atcggcgggc cgtaggtgac gttctgcgcg
    16801 acgatgacga cgcgcttctt ctgcgtgaac accgcctcgc gcatgagcgc gatcacctcg
    16861 tcggggtagc ccgagcggaa ggggaagaag ttgggcgcgc tgacgatgct cttgtcgccc
    16921 tggaagggcg cgatcatcgc gattccggac ttgccggcga tgtcctgctt cgcgacctcg
    16981 gtgatgccgc cggtgttctg gtagccggcg agggcgagca cgttgcggtc ggcgatgagc
    17041 tcgtgggtga gcgcgatcat cttgtcggtc ttgagctcgt cgtccttgtt gacgagcttg
    17101 accttgcggc cgtcgacgcc gcctttcgcg ttcacgtact cgaagtaggc ggtgatgccg
    17161 gcgtagagcg ccttggcatt cgccgcggtg accgagctgg tctgcgagga gacctggccg
    17221 atgaggatgt cctgcgcctg ggcggcgagc gcgaacccgg cggcgagggc ggcggcggcg
    17281 attcgtgttt ttgtggccat gctcccctct ccgggaatgt caggcgaagt gaaaccaata
    17341 taccgggttc cccggccggg agcgtcagga aagtccgacc gcttcggaaa aattcacgcg
    17401 gcctgcatcg gcgtcggaaa acgcgggcgg atctatctcg accagccggc gtctggggat
    17461 tgggtgcttg agactttccc gccggcgtcg aagaagacgg agccgcggcc cacgcccgga
    17521 tagacgtagt accagaccgg gctgccgtcg atgcggagaa gcttgtcggg cttgccgagc
    17581 aggctctcga cgcgctcggc gggaacgccg cgctcgaccc gcgaccagct cagccgcagc
    17641 tgtgcgagcg gcgagaggtc gggccgcagg ggcgccgccg ccgcggcggg gccgtccgcg
    17701 cggacggcgc catttccgac tccgcgctcg agcgcgcgta ttcgcgcgtc gagggcatcc
    17761 acctcgagat gcagccgagc cgattcggcg cgcaggcgct cgagctcctc ggccggatcg
    17821 gcgcgcgcgg ggcctgccgc gcacacggcg gcgacggcgc aagcgaggag gacccgtctt
    17881 cgcatcccgg ctagcgcgat gcgaggtagg cggcgaccgc ctccatctgc tcgaacgaca
    17941 tgccgcggct cacgccgtgc atcacgggag ccgtgccttc ctcgcgcagc tgctccttga
    18001 acagcacgag ctgcttgacg atgtactcgg cgtgctggcc ggccaggcgc gggaaggcgc
    18061 tcgtaccctg ggcgtccttg ccgtggcagg ttgcgcaggc ctggacctgc gcggcgggaa
    18121 cgcccttctc gaagatgtcc ctgccggcct gggcgagcgc ggttctcgcc ggagcgctgt
    18181 gcacggcctt ctgccgggag tagtagtcgg ccagcccgcg gatcgtggca tcgtcgagct
    18241 gcgacgccat gccccacatg tacgcctgag ccatcgggtc ggcgcgcgta cggtcgcgga
    18301 aggccttcag ctgggcgtcg agatactcga cccgctgcgc ggcgagtcgc gggaatgcgg
    18361 gcgagacgct ctcgccgcgc gccccgtggc acgcggcgca ggtctgcgtc gcgatcttcg
    18421 ctgcgtcagc gtcctgggcg ccggccgggg ccgcgaggca tgcgagcgcc acgatcgagg
    18481 cggataccgg aatcttgatg cgcatgttgg tctcctcgct agaacgccag ccagccgagc
    18541 aggtacagcg tattgttgtc cttcgcgttg cggccgctgc cgtcataatc ggtcttggcg
    18601 ccgttgaact tgtcgtaggc gatgtactgc agcgcgactt tgacgttctg ataaggcacc
    18661 cagtcgagct cgccgatcca gccgttgctg ttcgggctgc cattcgcgaa cccggtgacc
    18721 gccgccatgc cgcgcagcgt cgcgtccgcg ctgcccgtcg tcgagaagta tccgagcgcg
    18781 ccgccgtagt tgcgtcggta gtagtagctc gcgccgactc tgaaggtctt cagatcgttg
    18841 gtcgcgttgt ccgagccggc cgctccgaag gtcgcgtcga gcgtctgctt ctccttgatg
    18901 taggtcgtct gcccggacag gatgtgctcg tcgccgatga actggtactg cgcgtcgacc
    18961 gcggtgtcga cgaacttgtc ggccggcgga ccgaccggct gtccctcggg gctgatcttg
    19021 gcggtgaggc cgtaggcgcc gaccgagagc gagttgcggt cccattggtg ctcgtaggcg
    19081 acccgccagt acggggcgtt gctgatcagc gtgccgtccg cgttcgtgct gtcgagcgga
    19141 cccgcgccgt tgcggaactc gtggccctgc ggcgcggacc ggtacaggcc gagctcggcg
    19201 tagaccgagt tcttccacca tagatatccg gtcaggccgg cgacgcggcc cgcgagcccg
    19261 ccgtcgatca gcgtcgcggc gccggggacg ggggcggcgt tcgagcgctg gtcgaacggc
    19321 gtctgccacg ccggcgtgct gttgaagatg tcctgcaccg tggggttgtt gttcatcgtc
    19381 aggccccagg tgagggggct cgagagcagg ctcgcctgct gcgcgtagcg cagctcggtg
    19441 ttgtcccagt ggacgttgcc cgcgacgctg tcgtacgtga tctgcacgaa gctgccgagg
    19501 ccgggcgcga cgcgtccggc gtagaacagg ctcgcctgct gcgggaagag cacttggccg
    19561 ttctgggagt tcccgtcgat gccggcaccg gaatccggca ccgccttgct cgtccgcgtg
    19621 tacgacgtct gccacatgaa cgagaacggc gggacctggt tcagcagcag ctcgtcttct
    19681 ttcgactggc tgatgccttg cacctgcggc ag
//
LOCUS       DATDMW010000305         5014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1461902, whole genome shotgun sequence.
ACCESSION   DATDMW010000305 DATDMW010000000
VERSION     DATDMW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461902"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VI319_12870"
     CDS             <1..483
                     /locus_tag="VI319_12870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6864781.1"
                     /translation="GLSASSIGVVLAMQAVAAFCVRLWMPRLAMRFGERRLLAASLAM
                     AGVTYFAFPMFENPFVLGAISFFLGLGLGSGQPLSIILTYNDSPPGRAGEALGMRLAV
                     NKFTQIAVPVVFGSLGTAGGVFPVFWSNAVLLLIGGWMTRTRAPNRPAGAPVEGPRAP
                     "
     gene            complement(548..1906)
                     /locus_tag="VI319_12875"
     CDS             complement(548..1906)
                     /locus_tag="VI319_12875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6864782.1"
                     /translation="MEIRFLGATGTATGSKYLLRSGRASVLVDCGLFQGFKQLRLRNR
                     APMPVEADAISAVVLTHAHIDHSGYLPLLVRSGFRGRVYCSEATRDLCRILLPDSGRL
                     QEEDADYANRHGFSKHKPALPLYTEEDAKRCLKNFSPVPFSRETAVADLRVSLLPAGH
                     ILGASIVRVEHGERVLVFSGDLGRPEDPIMAPPSEIRAADALVVESTYGNRRHDRADP
                     KIKLGEVIRRTSARGGVVVIPSFAVGRAQSLLYAIHRLKSEGAIPAGLPVYLNSPMAA
                     DVTAIYRAHRGEHRLSAEQCDAMCRAAAVVGSAEDSKRLNERKGPMVILAGSGMATGG
                     RVVHHLKTFAPDPRNTILFAGFQAGGTRGAAILSGSDSVKIHGRYVPVRAEVDSLENL
                     SAHADGAEILDWLRRFERPPRQTFVTHGEPAAADAMRLKIEETLRWRVRVPDYLESAE
                     IG"
     gene            complement(1926..3479)
                     /locus_tag="VI319_12880"
     CDS             complement(1926..3479)
                     /locus_tag="VI319_12880"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6864783.1"
                     /translation="MSALPQALLDPACYPDAMDGVELLETHISWVFLAGEYAYKVKKP
                     VRLPFLDFGTLAARRRFCEEELRINRRTAPELYLGVVPIVSGGAGWRVDAPGEPAEYA
                     IRMRRFPQDALADRVAKRGALGAAQVDALAAAIARFHAAIPAAPPGSLLGDPERVTGP
                     VLANFEEITASPGDAPRLEGLRRWTRREGGRLREAFAGRKRDGFVRECHGDLHLGNIV
                     FLGGRPLLFDGIEFSAELRWIDVMSEAAFLVMDLLEHGLDSAAWRFLNAYLESTGDYA
                     GVRVLRYYLVYRAMVRAKVIRMRELDGAYLRYLALADFLAVSARPALVLMHGLAGSGK
                     TTVAQALLERVGAIRVRSDVERKRLFGLEPASRTHSELYAGIYALEATQRTYERLRQV
                     VRDVVGSGRTVIVDAASLRREDREGFAALARELDASFLIVSCRASHAELRRRVTLREV
                     AMRDASEAGVEVLERQIATQEPIADDELPHVGAVDTEGDLRAAVDALAARLSRAEAPA
                     AARPSTTEA"
     gene            complement(3476..4378)
                     /locus_tag="VI319_12885"
     CDS             complement(3476..4378)
                     /locus_tag="VI319_12885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6864784.1"
                     /translation="MDTDLYDAPQLQAVPSRWFEQSPDPVLVTDPDGTIRYVNPAFER
                     VTGYGRGEALGRTPAMLKSGIHEPDFYRGLWQQLLAGKPFRAVFVNRRKGGELFHAEN
                     VIWPLIDADGRIAAIVCESRDVSERVRSIEKLAHAATHDPLTDLPNRTLFLDRLGQAL
                     RQATRRNETVAVAIMDIDRFRDTNNIFGHPAGDAVLRAVARRTEAYVREADTVARIGG
                     DELALLLPGADDHAAAVLEKVRAANAVPVEFDERSIGVSVSIGACNFPTDGRDVEDLH
                     KRADVAMYEAKRSGGNRVRFYGDK"
     gene            4638..>5014
                     /locus_tag="VI319_12890"
     CDS             4638..>5014
                     /locus_tag="VI319_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEY6864785.1"
                     /translation="MHAAPDRAAQRHHAPSPGIEDGLTRREAVARLARHGPNVVAGER
                     AIAPLARLVRLFASPFPLLLLALAGAAQLSGEPRGAAVIAAIVALSVLLSWLEELRSG
                     RAAERLRALVHTTATVIRRDHAA"
BASE COUNT          680 a         1832 c         1772 g          730 t
ORIGIN      
        1 ggactgtcgg cgtcgtcgat cggcgtggtc ctcgcgatgc aagccgtcgc cgccttctgc
       61 gtgcggctgt ggatgccgcg gctcgcgatg cgcttcggcg agcggcgcct gctcgccgcg
      121 tcgctcgcga tggcgggcgt cacctacttc gcgttcccga tgttcgagaa ccccttcgtg
      181 ctcggcgcga tctcgttctt cctcgggctg ggcctcggca gcgggcagcc gctgtcgatc
      241 atcctcacct acaacgactc gccgccggga cgagcgggcg aggcgctcgg gatgcggctc
      301 gcggtcaaca agttcacgca gatcgccgtg ccggtcgtgt tcggctcgct cggcacggcg
      361 ggcggagtgt tcccggtgtt ctggtcgaac gcggtgctgc tgctgatcgg cggctggatg
      421 acccgcacgc gcgcgccgaa ccggccggcg ggcgcgccgg tcgaagggcc cagggccccc
      481 tgacccgggt cgacgcgccg cgagcggcgc ggctgtgccg gtcatgatgt cgcatcagga
      541 ccgcggatca accgatctcg gcgctctcga gataatccgg aacccggacg cgccagcgca
      601 gcgtctcctc gatcttcagc cgcatcgcgt ccgcggcggc cggctcgccg tgggtgacga
      661 aggtctgccg cggcgggcgc tcgaaccgtc gcaaccagtc caggatctcg gcgccgtccg
      721 cgtgcgccga gagattctcg agggagtcga cttcggcgcg gaccgggacg taccggccgt
      781 ggatcttgac cgagtcggaa ccggagagaa tcgccgcgcc gcgcgtgccg cccgcctgga
      841 accccgcgaa gagaatggtg ttgcgcggat ccggggcgaa cgtcttgaga tgatggacga
      901 ctcgcccgcc ggtggccatg ccgctcccgg cgaggatgac catcggtcct ttgcgctcgt
      961 tcaagcgctt cgaatcctcg gcgcttccga cgacagcggc ggcgcggcac atcgcatcgc
     1021 actgctcggc gctcagccgg tgctcgccgc ggtgcgcgcg gtagatcgcc gtcacgtcgg
     1081 ccgccatcgg actgttcagg tacaccggca acccggccgg gatcgcgccc tcggatttga
     1141 gccggtggat cgcgtagagc agcgactgcg ctcggccgac ggcgaacgac gggatgacga
     1201 ccacgccccc gcgcgcgctg gtgcgccgga tcacctcccc gagcttgatc ttcgggtccg
     1261 cccgatcgtg ccggcggttg ccgtacgtcg attccaccac cagcgcgtcc gccgcgcgga
     1321 tctcgctcgg gggcgccatg atggggtcct ccggtcgccc gagatcgccg gagaagacca
     1381 ggacgcgctc gccgtgttcg acgcgcacga tcgaggcgcc gaggatatgg ccggcgggca
     1441 gcaagctgac gcggagatct gcgacggcgg tctcgcgcga gaacgggacc ggggagaaat
     1501 tcttcaggca gcgcttcgcg tcctcctcgg tgtagagcgg cagcgccggc ttgtgcttcg
     1561 agaaaccgtg ccggttggcg tagtcggcgt cctcctcctg caagcggccg ctgtcgggaa
     1621 ggaggatgcg gcacaggtcg cgggtcgcct cgctgcagta cacccggccc ctgaagccgc
     1681 tgcgcaccag cagcggcaga tagccgctgt ggtcgatatg cgcgtgcgtc agcacgacgg
     1741 cggagatcgc gtccgcctcg accggcatcg gcgcccggtt gcgcaggcgc agctgcttga
     1801 agccctggaa caggccgcag tccacgagca cgctggcgcg cccgctccgc agcaggtact
     1861 tggagccggt ggcggtcccg gtcgcgccga gaaaacggat ctccatggcg ccccgcgtgc
     1921 gggagtcacg cctccgtcgt ggagggccgc gccgcggccg gcgcctccgc gcgcgacagc
     1981 cgtgccgcga gcgcgtccac cgcggcgcgc aggtcgccct cggtatcgac cgccccgacg
     2041 tgcgggagct cgtcgtccgc gatcggctcc tgcgtcgcga tctggcgctc gagcacctcg
     2101 acccccgcct cggatgcgtc gcgcatcgcc acctcgcgca gcgtcacgcg acgacgcagc
     2161 tcggcgtgcg aggcgcggca cgagacgatc aggaacgagg catccagctc gcgcgcgagc
     2221 gccgcgaagc cctcgcgatc ctcgcgcctg agcgacgcgg cgtcgacgat caccgtgcgg
     2281 ccggagccga ccacgtcgcg cacgacctgg cgcagccgct cgtacgtccg ctgcgtcgcc
     2341 tcgagcgcgt agattcccgc gtagagctcc gaatgcgtgc gcgacgccgg ctcgaggccg
     2401 aacaggcgct tgcgctccac gtccgagcgc acgcggatcg cgcccacgcg ctcgagcagc
     2461 gcctgcgcca cggtggtctt gcccgagccc gcgaggccgt gcatgagcac gagcgccggc
     2521 cgcgccgaca ccgcgagaaa gtcggcgagc gcgagatagc gcaggtacgc gccgtcgagc
     2581 tcccgcatcc ggatcacctt ggcgcgcacc atcgcgcggt acacgaggta atagcgcagc
     2641 acacgaacgc cggcgtagtc gccggtcgac tccaggtagg cgttgaggaa gcgccacgcg
     2701 gcggagtcga gcccgtgctc cagcagatcc atgacgagga acgccgcttc gctcatcacg
     2761 tcgatccagc gcagctccgc cgagaactcg atgccgtcga agagcagcgg ccggccgccg
     2821 agaaacacga tgttgcccag gtgcaggtcg ccgtggcact cgcgcacgaa cccgtcgcgc
     2881 ttgcgcccgg cgaaggcctc gcgcaagcgc ccgccctcgc ggcgcgtcca gcggcgcagc
     2941 ccttcgagac gcggggcgtc gcccggcgag gccgtgatct cctcgaagtt cgcgagcacc
     3001 gggccggtca cgcgctcggg atcgccgagc aggcttcccg gcggcgcggc cgggatcgcc
     3061 gcgtggaacc gtgcgatcgc cgccgcgagc gcgtcgacct gcgccgcgcc gagcgcgccg
     3121 cgcttcgcca cgcggtcggc gagcgcgtcc tgcgggaagc gccgcatccg gatcgcgtac
     3181 tccgcgggct cgccgggcgc gtcgacgcgc cagcccgcgc cgccgctcac gatgggcacg
     3241 acgccgagat agagctccgg cgccgtgcgc cggttgatgc gcagctcctc ttcgcagaag
     3301 cggcgccgcg cggcgagcgt gccgaagtcg aggaatggca gccgcacggg cttcttgacc
     3361 ttgtacgcgt attcgcccgc gaggaagacc catgagatgt gggtctcgag cagctccacg
     3421 ccgtccatgg catccggata gcacgccgga tcgagcagcg cttgcggcag cgcgctcact
     3481 tgtcgccgta gaaacgcacg cggtttcccc ccgagcgctt cgcctcgtac atcgcgacgt
     3541 cggcgcgctt gtgcagatcc tcgacgtcgc ggccatcggt ggggaagttg cacgccccga
     3601 tgctcaccga taccccgatc gagcgctcgt cgaactcgac cggaacggcg ttcgccgcgc
     3661 gcaccttctc gagcaccgcg gccgcgtggt cgtccgcgcc cggcaggagc agggccagct
     3721 cgtccccgcc gatgcgggcg accgtgtccg cttcgcgcac gtacgcttcg gtgcggcgcg
     3781 ccaccgcgcg cagcacggcg tcgcccgcgg gatggccgaa gatattgttc gtgtcgcgga
     3841 agcggtcgat gtccatgatc gcgacggcca ctgtctcgtt gcgccgcgtc gcctggcgca
     3901 gcgcctggcc caggcggtcc aggaacagcg tgcggttcgg caggtccgtc agcggatcgt
     3961 gcgtcgcggc gtgagcgagc ttctcgatgc tgcgcacgcg ctcgctgacg tcgcggctct
     4021 cgcacacgat cgccgcgatg cggccgtcgg cgtcgatgag cggccagatg acgttctccg
     4081 cgtggaacag ctcgccgcct ttgcgccggt tgacgaacac cgcgcggaac ggcttgcccg
     4141 cgagcagctg ttgccacaga ccacggtaga agtcgggctc gtggatgccc gatttcagca
     4201 tggccggcgt gcgcccgagg gcttccccgc ggccgtagcc ggtgacgcgc tcgaaggcgg
     4261 gattcacgta gcgtatcgtt ccgtccgggt cggtcacgag caccgggtcc gggctctgct
     4321 cgaaccaccg gctcgggacc gcctgcagct gcggtgcgtc gtacaagtcc gtgtccattc
     4381 gcgtctcccc gggcgcgatc caccccgcca tggtcgccca tcggccgcct ccgcatttga
     4441 cgtgcatcaa attgaggcgg cgccggacga tctatcttcg ggcgaacgct catggaggcg
     4501 acgatgtacc gccggatccc gattcccttc cacccaagcc cgaagtgcgc ccgctccctg
     4561 tgatcgacga gctctacgcg ttcgcggcgc cggaaggcct ccgcggacgg ccctaggcgg
     4621 ctgcggctgt gccgtccatg cacgccgccc cggaccgcgc ggcgcagcgc caccacgcgc
     4681 catcgcccgg gatcgaggat ggcctgacgc gccgtgaggc ggtcgcgcgg ctcgcgcgcc
     4741 acggaccgaa cgtcgtcgcc ggggagcgcg ccatcgcgcc gctcgcgcgc ctggtccgtc
     4801 tttttgccag ccccttcccg ctgctgctcc tggcgctcgc cggggccgcg cagctgagcg
     4861 gcgagccgcg cggcgccgcg gtcatcgccg ccatcgtggc cctgtcggtc ctgctgtcgt
     4921 ggctcgagga gctgcgctcg ggccgcgccg ccgagcggct gcgcgcgctc gtgcacacga
     4981 ccgcgacggt gattcgccgg gaccacgcgg cgga
//
LOCUS       DATDMW010000306        25600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1213154, whole genome shotgun sequence.
ACCESSION   DATDMW010000306 DATDMW010000000
VERSION     DATDMW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25600
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213154"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VI319_12895"
     CDS             <1..532
                     /locus_tag="VI319_12895"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY6864786.1"
                     /translation="NIEMPATPQRVWQALRLRERIAERSLVRHREAADLVVAEVDAGG
                     REHRLVPAAARAWSALCAAARGDGVPLRIVSAFRSIDRQAAIVRAKLERGIPIDEILR
                     VSAPPGYSEHHSGCAVDVTTDGVRPLEEEFERTAAFEWLQRNAGRFGFRLSFPRGNDQ
                     GYAYEPWHWCWHGPGN"
     gene            605..805
                     /locus_tag="VI319_12900"
     CDS             605..805
                     /locus_tag="VI319_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864787.1"
                     /translation="MSKIEQIEKQIQSLSEQELAEFRTWFAEFDHGLWDRQIEADSRS
                     GKLDAMAAQALADYNAGKARKI"
     gene            802..1056
                     /locus_tag="VI319_12905"
     CDS             802..1056
                     /locus_tag="VI319_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864788.1"
                     /translation="MKHYASPAFWARYDALPTEIRGLADKNFELLKTDPRHPSLHFKK
                     LGDHWSARVGAHYRALGMEVEGGMYWFWIGTHAEYDKLIG"
     gene            1059..2345
                     /locus_tag="VI319_12910"
     CDS             1059..2345
                     /locus_tag="VI319_12910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6864789.1"
                     /translation="MAVMTNKQLDYDVAVIGGGPGGSSAATALVRRGRKVLLLERDKF
                     PRFHIGESQLPWNNEVLRELGAFEAITGGGFMPKWGASFRRPDGVTEQYADFAHAVET
                     PTPLSYQVDRERFDDILLRHAARSGADVRERHRFLDAAFDADGVTVKYADADGAERSA
                     RVAAVVDASGRAGVIVNRFGRHEYDPMLQNIAIHAQVVGVPRAEGRRAGDIRMFTRPD
                     MGWLWFIPLTDTVMSIGAVIPKATHRREAKASAEASLDHYFAATPQAAALLERAKRTS
                     PARVDVDYSYLATKMAGERWVAVGDSAAFLDPIFSTGVLLAMQGGIEAAEAIDAGLTK
                     GDLSAHAFARYERVVRKRYHHFRRFAVGFYDPAFREVWFAEKKRFGLYPAIVSILAGN
                     WKPSLATRARIALFFGLIALKRLGRKDLALPAAPAR"
     gene            complement(2342..3214)
                     /gene="htpX"
                     /locus_tag="VI319_12915"
     CDS             complement(2342..3214)
                     /gene="htpX"
                     /locus_tag="VI319_12915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY6864790.1"
                     /translation="MKRVFLFLVTNLAVLLLLSVVLRVLGVDRFLTANGIDYAQLLVF
                     SVIVGFAGSIFSLLISKQMAKWSTGARVIDEPQGAHERWLVDTIRMLAQKAGVGMPEV
                     AVYEGAPNAFATGAFRDSSLVAVSTGLLNSMNREEIEAVLGHELSHVANGDMVTLTLI
                     QGVVNTFVVFCSRIAGYIVDKAVFRTERGVGPGYFVTVIAFQIVFGILASLIVAWFSR
                     RREFLADAGSARLLGPQPMIHALQRLGALEHGELPQSMSAFGIAPSSSGWMAMFASHP
                     PIEDRIRALQAPGA"
     gene            complement(3390..3590)
                     /locus_tag="VI319_12920"
     CDS             complement(3390..3590)
                     /locus_tag="VI319_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864791.1"
                     /translation="MKRTIAILMAALFAGTTLSAIAQGTAPAPADTPKAEKKVEKKEK
                     KAAKKAAKKEKKAAKKAEKKEG"
     gene            3751..4611
                     /locus_tag="VI319_12925"
     CDS             3751..4611
                     /locus_tag="VI319_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEY6864792.1"
                     /translation="MRIAFVGAGRLGSGLARAFGRAGVQVVAVSSRSPAAAEALARDI
                     PGARAVPAQEAIALADIVFLSVPDDAIEPVAAGLRWAPGKACVHCSGAGELDLLHKAA
                     GEGAAIGGFHPLRMFARPGEAVASLDGAGVAIAGSEPLAAELERLARAIGGRPFRLPP
                     GSRALYHASANFVGGFVIALVQEALDLWKRLGIPEEQALAALLPLARGTLDNVEAFGP
                     AGGLGSAVARGDVGTIRKHLDALAEQAPGSLELYRLLSLRTLPLGLAKGTLSPEAARA
                     LEALLSRPGV"
     gene            4620..5954
                     /locus_tag="VI319_12930"
     CDS             4620..5954
                     /locus_tag="VI319_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEY6864793.1"
                     /translation="MRRVWLLIALVLAPTWVFAGPVVVLTVTGAIGPATADYVHRGIE
                     RAGRDGAELVVLRMDTPGGLDTSMRAIIKDILASPIPVASFVAPGGARAASAGTYILY
                     ASHVAAMAPATNLGAATPVAIGGGSRGGDEPVSAPDKGKAAKPAPAGDTMTRKQVNDA
                     AAYIRGLAEMRGRNADWAERAVREAVSLPASEALKLKVIDLIAADVPDLLKRLDGRKL
                     ATAAGEKTLATAGAAVVSLEPDWRTRFLSVITDPSFAYLLVLAGIYALFFEFSNPGLV
                     FPGVAGAICIVVALYAFHLLPVSYAGLALVLLGIAFMAAELFFPAYGSLGVGGAVAFV
                     TGSVMLIDTDVPGFSLPLPLILGVAAVSVAFVFVVVGSALKARRRPVVSGAEQMVGST
                     GEVLADCDREGWARVHSETWRIRCATPLKAGQRVRVARMEGLLLDVVPDPGA"
     gene            5960..6724
                     /locus_tag="VI319_12935"
     CDS             5960..6724
                     /locus_tag="VI319_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY6864794.1"
                     /translation="MFALNFGTVFLVVVLVVVFWSFRIMREYERSVVFQLGRFWRVKG
                     PGLIIVWPVLQQAVRVDLRTIVFNVPPQDVISRDNISVKVDAVVYFRVVDPQKSIIQV
                     ADYLTATSQLAQTTLRAVLGKHELDEMLAEREKLNMDIQRALDTQTDAWGIKVSNVEI
                     KQIDLNETMVRAIARQAEAERDRRAKVIHAEGELQASVKLLEAAQMLAKQPEAMQLRY
                     LQTLTQIAGDKSSTIVFPLPMDILTPLVDAMKKRNA"
     gene            6734..7504
                     /locus_tag="VI319_12940"
     CDS             6734..7504
                     /locus_tag="VI319_12940"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY6864795.1"
                     /translation="MDESAVYTEPTFVDRVRGPAWIALAGFALAALAVLAVLAGGWGN
                     RAGSWDYRVAFTILRWAAWIGLAGGLAAGIGGTLAISGAPPRRVSVLAIAGVVLGALG
                     FGIPWAYTAKRSAPINDITTDPANPPRYVDIAPLRASAPVKADYGGEETAAIQKKTYP
                     DIAPALLDVSREQAYERALAAAKSLGWTIVAAVPAEGRIEATDTTLFMGFTDDVAIRV
                     QAEGAGSRVDVRSHSRVGRGDFGTNAKRVRAFLAKLGR"
     gene            complement(7501..7899)
                     /locus_tag="VI319_12945"
     CDS             complement(7501..7899)
                     /locus_tag="VI319_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6864796.1"
                     /translation="MKASLAPGLATTTKIRVDEPRVISFMGDDCRVYATPRIISDVEY
                     TCRDFLLAHLDPGEDSVGTQVHWHHVGPALLGAEVTLDIRVASVDGRRVTFEAKVSDG
                     ADAVAHGTHERFVVDVQKVRERLLKKAKKP"
     gene            complement(7896..8807)
                     /locus_tag="VI319_12950"
     CDS             complement(7896..8807)
                     /locus_tag="VI319_12950"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY6864797.1"
                     /translation="MAADIASVLIYGYGVMGRGVARTFAEGGFDTVVKSSRARELADL
                     PAKVRAVDRLPAEAPDLVIEFVPEDVATKQAVYAEIEAAYPGKDVLIATGTSGLDLVK
                     LSAKMKDPARFVGIHYFMPADTTPVVEVMAGPSTPREAVDRAAAALERTGKETVVLYK
                     PIVGFLINRLQHAILHEAYYLVEAGVASAADIDRAAMHMLAPRMCLNGLIQQKDISGL
                     RIHADAQRSIVPKLFHNGAPNSMLQNMVKRGHEGLSAGRGFYDWSGCDKDLVRRLASS
                     RLAKLLAFLHDGIEPLPERARPKDAAP"
     gene            complement(8819..9853)
                     /locus_tag="VI319_12955"
     CDS             complement(8819..9853)
                     /locus_tag="VI319_12955"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY6864798.1"
                     /translation="MSPERLQSFTSTVLEKVGLARADAQTCARLMVRADLHGADGHGV
                     FRLPQYVRRIQGGAVNVAPRVRIEREAAGMALVDGDNGMGHVVMSFAAQTAIAKAKTA
                     GAAWVGVRASNHAGPASLYAAMPIEHDMIGLYLAVGNANHMAPWGGIDLLLSTNPIAI
                     AVPGERAPIVLDMATTVAAYGKVKTKAQRGETMPEGWMIDREGRPLTDPKRSNEGLLL
                     PIGGYKGYGLSLMIGLLAGTLNRAALGSQTIDFNADDTSVTNTGHAIVALSLAAFGDV
                     AAFKRDVDRVAGELRASRRLPGVERVWLPGEQSRAKYEERSRHGVPVPAALRDALDRL
                     AREVGVAPLD"
     gene            9904..10320
                     /locus_tag="VI319_12960"
     CDS             9904..10320
                     /locus_tag="VI319_12960"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY6864799.1"
                     /translation="MAGNLHPELRVGLAGSAELVVGEEHTAPRVGSGRVHVLATPVMI
                     NLIEAAALAAVEHLLAPGYQSLGTLLNVRHIAATPVGMRVRATAEVVKIDGRNVTFRV
                     EAHDERDLIGDGAHERVIVNVERFDKRVQEKLQAMR"
     gene            10439..10678
                     /locus_tag="VI319_12965"
     CDS             10439..10678
                     /locus_tag="VI319_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864800.1"
                     /translation="MTRFRTVSLLLAVAGGLLVAAPAFADEVILKSGERITGRIVEKS
                     SEALVLETASADNVRVAWADIFSLKTDAPQDAMLT"
     gene            10838..11317
                     /locus_tag="VI319_12970"
     CDS             10838..11317
                     /locus_tag="VI319_12970"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEY6864801.1"
                     /translation="MKGSLKRAAVAALVGGAIAVPALAQDQTPQPAPGQHHGWRHANP
                     EARMEHMVTRMFSSVNASDQQKTQAVTIVRAAAADIRPLEQQLRDGRRAAMQLLAAPA
                     IDRNALEAQRVQQSAVQEQISKRRTQAVADLAEVLTPDQRAALAKRMSERFGGRRAG"
     gene            11433..12140
                     /locus_tag="VI319_12975"
     CDS             11433..12140
                     /locus_tag="VI319_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6864802.1"
                     /translation="MTAHILIIEDDERLSAMVAEYLHESGFLVSARAEGGEGLALLER
                     EPFDALILDLMLPDLDGFEVCRRVRARSDIPILMLTARGEATDRVVGLELGADDYLPK
                     PFNPRELLARLRAILRRRKPSGPPEPALRFGRLESDRGARVVRLDGEEKPLTAYQFAL
                     LVALAENAGRIMSRESLMDLVKGEPLEAFDRSIDVHISRIRAAIEDDPKRPRRIITVR
                     SAGYVFAKVQDDREDAS"
     gene            12137..13420
                     /locus_tag="VI319_12980"
     CDS             12137..13420
                     /locus_tag="VI319_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6864803.1"
                     /translation="MTPPRPRRLYRQFYVTLVASLALFAVIAGFLWHTLTEASPARQA
                     FEILSELAQASLAEAGAPRDAQQAALNDLRARVRGRFAGLALYAADGERIAAAGAPVP
                     RPGPGRQAGGWIPGPEGRHAWAIALPDGRVLIARLPRPRPNPMLGLFFILGLITLGVA
                     VGAYPVVRHLTRRLERLQASVEALGAGDLSARVPVDGADEVSALASSFNRAAERIEQL
                     VAAHKTMLANASHELRSPLARIRMGIEILRADARPELREELARNIAELDALVDEILLA
                     SRLETVKQPDAVDTVDLLGLLAEECARADAALEGEPVTVRGDARLLRRAARNLLENAR
                     RHGGGPVEATVRRTPAAAAVIEVCDRGPGVPEAERERIFEPFYRPAGTRERSGGVGLG
                     LSLVRQIARKHGGDARCLPREGGGSRFVLELGTAT"
     gene            complement(13442..15025)
                     /locus_tag="VI319_12985"
     CDS             complement(13442..15025)
                     /locus_tag="VI319_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6864804.1"
                     /translation="MLISSTFQNLGLSAELLKAVAEQGYTEPTPIQAQAIPVVLSGRD
                     LLAAAQTGTGKTAGFTLPMLQRLNATRAAPGPRPVRALVLVPTRELAAQVQQSVRDYG
                     RHLALESVVIFGGVGFTPQADNLADGVDIVVATPGRLLDHVQERTIDLGRIEILVLDE
                     ADRMLDMGFIPDIRRILALLPAKRQNLLFSATFPDEIRKLAASFMHDPATVEVARRNA
                     PIELITQVAHPVDRERKRALLEHLIKSNEWTQVLVFTKTKHGANRLAQQLVRSKIEAD
                     AIHGNKAQNARTRALKAFKENELRVLVATDIAARGLDIELLPHVVNYDLPNVPEDYVH
                     RIGRTGRAGETGEAISLVTPEDRPLLAAIERLINRRIESRVVPGFEPGTAAQQPQQQR
                     EPQQQQRRPQQQPPQQQGQRGRREGRRHRQQQGNGQQRPHQPQQNRQPQRDANRGKMR
                     GRPGPQQPRGLPRADEQERLAQIEYARRMSQADEESRNDAAQQPQQEQQEQQPAAPRR
                     DRPRQVPALFNSFGRKNGG"
     gene            complement(15185..15445)
                     /locus_tag="VI319_12990"
     CDS             complement(15185..15445)
                     /locus_tag="VI319_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864805.1"
                     /translation="MSSAKEDEARQSVAELAAAIYVELVGRAFLRVDNAATIKPEAAV
                     LAKLSFELAAAFQESAKTSLAALGPQNVGYNVQLGDIAGWHK"
     gene            15577..16185
                     /gene="gstA"
                     /locus_tag="VI319_12995"
     CDS             15577..16185
                     /gene="gstA"
                     /locus_tag="VI319_12995"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEY6864806.1"
                     /translation="MKLYYAPGACSMAPHIVLREAGYQFDLEKVDIPNKKTASGDDFW
                     KVNPKGYVPALRLDDGQVLTEAQVIVQYLADHKQDAGLAPRGGTMERYRLMEMLNFTA
                     AEVHKQLGALFNPKMTPEAKEIQLGVIDRRLTALDKMLAGKQYATGDKFTVADAYLFT
                     VLNWTNFHKIDTSKWPNIQAFMKRVAARPKVQETLKAEGLTQ"
     gene            complement(16206..16565)
                     /locus_tag="VI319_13000"
     CDS             complement(16206..16565)
                     /locus_tag="VI319_13000"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY6864807.1"
                     /translation="MEIYQEFSFDAAHRFPGAPEGHRYHGVHGHSFTARVVVAGTPDA
                     LTGFVADLGELERACAGLRESLDHRFLNEVGGLEVPSLEHIAIWIWGRLKPAFPGLCR
                     VEVRRDSVRHGAVYTGA"
     gene            complement(16574..17203)
                     /gene="queE"
                     /locus_tag="VI319_13005"
     CDS             complement(16574..17203)
                     /gene="queE"
                     /locus_tag="VI319_13005"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEY6864808.1"
                     /translation="MAYSVKEIFYTLQGEGANAGRPAVFCRFAGCNLWSGREADRAGA
                     VCRFCDTEFVGTDGPGGGKFDDAGALADRIASLWPGAGARFTVLTGGEPALQLDEPLV
                     RALHARGFQIAIETNGTLELAPGVDWICVSPKAGTEVKVRRGDELKLVFPQEGAPPER
                     FEGWSFGHFLLQPMDGPEKRENTERAIAYCMRNPRWRLSLQTHKLIGIR"
     gene            complement(17217..18470)
                     /locus_tag="VI319_13010"
     CDS             complement(17217..18470)
                     /locus_tag="VI319_13010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY6864809.1"
                     /translation="MRPKLLLEHLRPVLAPVVVLVASAVIVWIGSLPDSLSGLTVLGP
                     YFVLLLGAIIAVWFNRGRAFVALASLFAAYAGYRIALEFGADSFTARAVFTALSVLVP
                     LNVLIALLFPERGVVQHRNYRWLLLALAEVLLVAWIASAGRSSLSGVAWHGVLDHWLL
                     RSPPAPIAGSIAFAAALVAALARAWPRAGVEVRPIDAGLAGALVAFFIACEWARSPGV
                     FPIFMSAAGIVLLVSILQESHRLAFVDELTGLPGRRALEERLSGLGPVYTVAMVDVDH
                     FKKFNDTHGHDVGDQVLKLVASRLAEIDGGGKAFRYGGEEFAVVFGEHPLSEALPHLE
                     AMRDSIERYRMAVRGLDRPKDPETGLRMRIPGTPSQADRTLSVTISIGVAERDGMLTA
                     PGQVIRAADAALYRAKRGGRNRVCR"
     gene            complement(18506..22201)
                     /locus_tag="VI319_13015"
     CDS             complement(18506..22201)
                     /locus_tag="VI319_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6864810.1"
                     /translation="MGKGTMPPDFLMGKTRWETGAQPVGCTWEEHRAELEAHRPFHDL
                     EMYWIDASGHEHFISISGSPIFDVDGGFRGYRGSGRDITERKRAEKVLRLEHTVGRTL
                     AEADNVSTALKTVMGAVCESQSLECGVYWTWDESKDAMAFAESYSVPGSSIEALDKAS
                     SVGTFRRGEGLVGVVWGSGMPLWIPDATRDARVKRPDLVTAVGVHGVFLFPVVSEGKV
                     IGVFDFSSRTVREPDERLLEAARVIGSQIGQFVHRKRAEKVLRESEERFRSLTELSSD
                     WYWEQDAEFRFTALAGNMPSPAARLLGKTRWELDNTAERDVDWIAHRETLEAHRPFRD
                     FVRPALDQTGTLRWLSVSGEPVFDERGAFRGYRGTGKDVTESVRREDELRRFRASMDL
                     SGDMIFLIDRERMRFIDMNETACRSLGYSREDLLQIGPQDLLPITRDSLERAYDEMIR
                     TGQMHSGMRSHYRRKDGSLLPFESTRRVLRSADRWIVVVISRDTSERIAAEEALRRSN
                     ERFNLAVRATNDIIWDWNLITDEVWWNDNLSKVLGYGREQVDPSNRFWYGNIHPDDRD
                     RVESGLAQLLDSGGANWSDEYRFRRADGTYAHVFDRGHVVYDDNGRPVRMIGAIADIT
                     RRKETEETVRSQALQQRLIAEFGQQALASADLEDVLNHAVELVSVTLKVDYCEVLELN
                     ADGKALTYRAAAGWPPEWVGRRVVQVRPGGMLAHVLASREPVVVEDHSAETRFPGSAL
                     MQFGIRSGMQVPIMGSQGAYGVLGVHRFTVHRFSRDDASFLQSVANILAVAIDRKSAE
                     EQLAHLAQFDVLTGLPNRYLFHDRLVQSIAQAKRAARAIAVLFIDLDRFKLVNDTLGH
                     RAGDTLLAEAATRLADCVRSGDTVGRFGGDEFGAILTDLGRPADASLVAQKIIDALSQ
                     PFQLDGQETFISASVGITIYPTDGEAPESLVMNADTAMYRAKEQGRNTHQYFTREMNE
                     RALARVQMEASLRRALERGEFLMHYQPKMDLATGAISGFEALMRWQHPERGIVSPLEF
                     IPVLEETALIIPAGEWAMREVCRQVKVWKNMGLDVPPVAVNLSARQFQHKNLDATVRR
                     ILYETEVDASLIQFELTESLLMKDPESAARTLKALKESGVAVSVDDFGQGYSSLAYLK
                     RFPLDALKIDRAFIRDVTIDPEDAAITFAIIGLAHSLKLRVIAEGVETREQLDLLFAH
                     GCDEIQGFWFASPASAQECERLLRKAA"
     gene            complement(22292..23800)
                     /locus_tag="VI319_13020"
     CDS             complement(22292..23800)
                     /locus_tag="VI319_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY6864811.1"
                     /translation="MESASTRRRAVLNAKRARRAQPHRADGGAPAWSEQFLAREKAIL
                     EMIARGRPVDAVLEALTRSVEAEVDGLLCSVLLVDRDGRRLRHGAGPSLPAAWREAVD
                     GIAIGPGESPCSRAAFSAERVIVEDLSTDPLWIERRALAERLGLRACWSVPILSGEKK
                     VLGTFAQYYRQPRAPSERDLALIDRVLNIAGIVLERSRAEDALRDSEERFRSLSKLSS
                     DWYWEQDENFRFTKMSDALLGRSGRVAAAAHIGKRRWELPTLNMTHADWEAHRALLEA
                     HQPFSDFEICRPDQEGNPCYASISGEPIFDDAGKFRGYRGVGKDITARKREEELLRLE
                     HSVARSLAEAESASTGLRAVIRALCETEVWDCGRYWRVDEADGVLRFGDAWGPPIPRI
                     QELIEASRGFVFRPGEGFAGKVLQTGEPLWVADVGSDPRAMYRASFAKGGIRAAFVFP
                     VTAEARTIGVIGISCARVRAPDDRLLQAVRVIGSQVGQFLKRKDIEQALRES"
     gene            complement(23964..>25600)
                     /locus_tag="VI319_13025"
     CDS             complement(23964..>25600)
                     /locus_tag="VI319_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEY6864812.1"
                     /translation="QWSVSDARKWVESLALTGRDTEIARDVVTEIKSRLEFLEEVGLG
                     YLTLDRAAPTLSGGEAQRIRLAAQLGSNLRGVCYILDEPTIGLHHRDNRILLDVLEKL
                     EAKGNSLVVVEHDEDTIRRAGHVIDLGPGAGKHGGRVTAQGTARDLVRNPASATGRCL
                     AHPLEHPLHPPRATGRDAPAVEILGATLHNLKNVDVRLPLARLVSVTGVSGSGKSTLA
                     RDILYANLAALLGAKRRKRAAAPAGCREIRGWESVGRVLEVDQTPIGKTPRSCPATYI
                     GFWDSIRRIFAETAEARMRGWTASRFSFNTAGGRCESCEGQGVKTIEMSFLPDVKVTC
                     EACGGARFNAETLAVRWRGKTIGEVLAMSVEEAVDFFAAHPSTHRALRLLQDVGLGYL
                     TLGQQSPTLSGGEAQRIKLVAELAKAGTAVPADGRRGRIASAVHTLYVLDEPTVGLHM
                     ADVEKLIRVLHRLVDAGNTVVVIEHNLDVIADADWIVDLGPEGGEAGGRIVEQGPPAR
                     LAARIVDPKRTSHTARALAEFLSTRKPADLEPAPAA"
BASE COUNT         3594 a         9012 c         9181 g         3813 t
ORIGIN      
        1 caatatcgag atgccggcga cgccgcagcg cgtgtggcag gcgctgcgcc tgcgcgagcg
       61 gattgccgag cgctcgctgg tccggcatcg cgaggccgcg gacctcgtcg tcgccgaggt
      121 cgacgcgggc ggccgcgagc accggctcgt ccccgcggcc gcgcgcgcgt ggagcgccct
      181 gtgcgccgcc gcgcgcggcg acggcgtgcc gctgcgcatc gtgtccgcgt tccgctcgat
      241 cgaccgccag gccgcgatcg tccgcgcgaa gctcgagcgc ggcatcccga tcgacgagat
      301 cctgcgcgtc agcgcgccgc ccgggtacag cgagcaccac tccggctgcg ccgtcgacgt
      361 cacgaccgac ggcgtgcgcc cgctggagga ggagttcgag cgcacggccg cgttcgagtg
      421 gctgcagcgc aacgccggcc gcttcgggtt ccggctttct ttcccgcgcg gcaacgacca
      481 ggggtacgcc tacgagccgt ggcactggtg ctggcacggg ccgggcaact gacactcatg
      541 ccgaaggcct gagcctgcct gccattttct cggacagcga tcggcgcggt gctaaaatga
      601 caccgtgagc aagatcgaac aaatcgagaa gcaaatccag tccctgtccg agcaggagct
      661 ggccgaattc cgcacctggt tcgccgagtt cgaccacggg ctctgggatc gtcagatcga
      721 ggccgattcg aggtctggaa agctggacgc gatggcagcc caggcgctcg ccgattacaa
      781 cgccgggaaa gcgcgaaaga tttgaagcac tacgcatcgc ccgcgttctg ggcgcggtac
      841 gatgctcttc cgaccgaaat acgcgggctg gccgacaaga atttcgagct tctgaagacc
      901 gatcctcggc atccatcgct tcatttcaag aagctcggag atcactggtc ggcgcgtgtc
      961 ggggcgcact atcgcgcgct cgggatggaa gtagagggcg ggatgtattg gttctggatt
     1021 ggaacgcacg ccgaatacga taagctgatc gggtgaggat ggcggtgatg acgaacaagc
     1081 aattggacta cgacgtcgcc gtcatcggcg gcggccccgg cggctcgagc gccgcgacgg
     1141 cgctggtgcg ccgcggccgc aaggtgctcc tgctcgagcg cgacaagttt ccgcgcttcc
     1201 acatcggcga gtcgcagctg ccctggaaca acgaggtgct gcgggagctc ggcgcgttcg
     1261 aggcgatcac gggcggcggc ttcatgccga agtggggcgc gagcttccgc cggcccgacg
     1321 gcgtcaccga gcagtacgcg gacttcgcgc acgcggtgga gacgccgacc ccgctgtcgt
     1381 accaggtcga ccgcgagcgc ttcgacgaca tcctgctgcg ccacgcggcg cgcagcggcg
     1441 ccgacgtgcg cgagcgccac cgcttcctcg acgcggcctt cgacgccgac ggcgtgacgg
     1501 tcaagtacgc ggacgcggac ggcgccgagc gctccgcgcg cgtcgcggcc gtggtcgacg
     1561 cctccggccg cgctggcgtg atcgtgaacc gcttcggccg gcacgagtac gacccgatgc
     1621 tgcagaacat cgccatccac gcgcaggtcg tcggcgtgcc gcgcgccgaa ggccgccgcg
     1681 ccggcgacat ccgcatgttc acgcggcccg acatgggctg gctgtggttc atcccgctca
     1741 ccgacacggt gatgagcatc ggcgcggtga ttcccaaggc aacgcaccgg cgcgaggcga
     1801 aggcgagcgc cgaggcgagc ctcgatcact acttcgcggc gacgccccag gccgcggcgc
     1861 tgctcgagcg cgcgaagcgc acgtcgccgg cgcgcgtcga cgtcgactac tcctatctcg
     1921 ccacgaagat ggccggcgag cgctgggtcg cggtgggcga ctcggccgcg ttcctcgatc
     1981 cgatcttctc gaccggcgtg ctgctcgcga tgcagggcgg gatcgaggcg gccgaggcga
     2041 tcgacgcggg gctgacgaag ggcgatctct cggcgcacgc ctttgcgcgc tacgagcgcg
     2101 tggtgcgcaa gcgctaccac catttccggc gcttcgcggt cggcttctac gacccggcgt
     2161 tccgcgaggt ctggttcgcg gagaagaagc gcttcggact ctatcccgcg atcgtcagca
     2221 tcctcgcggg caactggaag ccgtcgctcg ccacgcgcgc gcgcatcgcg ctgttcttcg
     2281 ggctgatcgc gctgaagcgc ctcgggcgca aggacctcgc gctgcccgca gcgccggcgc
     2341 gctaggcgcc cggggcctgc agcgcccgga tccggtcctc gatcggcggg tgcgacgcga
     2401 acatcgccat ccagcccgac gacgacggcg cgatgccgaa cgccgacatc gactgcggca
     2461 gctcgccgtg ctcgagcgcg cccaagcgct gcagcgcgtg gatcatgggc tgcgggccga
     2521 gcagacgcgc cgagccggcg tcagccagga actcgcgccg gcgcgagaac caggcgacga
     2581 tgagcgaggc gaggatgccg aacacgatct ggaacgcgat cacggtgacg aaatagcccg
     2641 ggccgacgcc gcgctcggtg cggaacaccg ccttgtcgac gatgtagccg gcgatgcgcg
     2701 agcagaaaac gacgaaggtg ttcacgacgc cctggatcag cgtcagcgtc accatgtcgc
     2761 cgttggcgac gtgcgagagc tcgtggccga gcacggcctc gatctcctcg cggttcatcg
     2821 agttcagcag cccggtcgac accgcgacca gcgacgagtc gcggaacgcc ccggtcgcga
     2881 acgcgttcgg cgcgccttcg tagacggcga cctcgggcat gccgacgccc gccttctgcg
     2941 cgagcatccg gatcgtgtcc acgagccagc gctcgtgcgc gccctgcggc tcgtcgatca
     3001 cgcgcgcgcc ggtcgaccac ttcgccatct gcttggagat gagcagagag aagatcgacc
     3061 cggcgaagcc gacgatcacc gagaacacga ggagctgggc gtagtcgatc ccgttcgcgg
     3121 tgaggaaccg gtccacgccc aggacccgca gcacgaccga gagcagcagc aggacggcga
     3181 ggttggtgac gaggaacagg aaaacgcgct tgatcatggg caggagctct ccgtggaggg
     3241 gtcgggacgc tagatggggg cgccgaccgg cttctcaagc cgggcccgat tgtagcgctc
     3301 cgggaactcc gacgtgccaa aagcaaaaag gcgggggagg cccgcctttt tgcgttccgc
     3361 ggcgctggac gccgcgcgct gaaaaaagct cagccttcct tcttctcggc cttcttcgcc
     3421 gccttctttt ccttcttcgc ggccttcttg gcggccttct tttccttctt ctcgaccttc
     3481 ttctcggcct tcggcgtatc ggccggagcc ggggcggtgc cctgcgcgat ggcgctcagg
     3541 gtcgtgccgg cgaaaagcgc ggccatcagg attgcgatcg ttctcttcat ggtgcttcct
     3601 tctctctgtc caaaataggg ggtgcccgat cctccgggca accgaactaa cgcgcgacgg
     3661 cccgggcggt tgacgcccgg ggccgccccc gggccggcgg gccctacaat ggacagcgct
     3721 tacaactttc ggtttctacc gggtctatcg atgagaatcg cgttcgtggg ggccgggagg
     3781 ctggggtccg ggctcgcccg ggccttcggc cgggcggggg tccaggtcgt cgcggtctcg
     3841 agccgcagcc cggcggcggc cgaggccctg gcgcgggaca tccccggggc ccgggcggtc
     3901 ccggcgcagg aggccatcgc gctggccgac atcgtcttcc tgagcgtccc ggatgacgcc
     3961 atcgagcccg tggcggccgg gctccgctgg gcccccggga aggcctgcgt ccactgcagc
     4021 ggcgcgggcg agctcgacct cctgcacaag gcggcgggcg agggcgccgc gatcggaggc
     4081 ttccatcccc tgcgcatgtt cgcgcgcccg ggcgaggccg tcgcctccct cgacggcgcg
     4141 ggcgtcgcga tcgccggctc ggagccgctc gcggcggagc tcgagcgcct cgcgcgcgcg
     4201 atcggcggcc ggccgttccg gctgccgccg ggcagccgcg ccctgtacca cgcgtcggcc
     4261 aacttcgtcg gcggcttcgt gatcgcgctc gtccaggagg ccctcgacct ctggaagcgg
     4321 ctcggcattc ccgaggagca ggcgctcgcc gcgctcctgc cgctcgcgcg cgggacgctc
     4381 gacaacgtcg aggcgttcgg gcccgccggg ggcctgggca gcgcggtcgc ccgcggcgac
     4441 gtcggcacca tccgcaagca cctcgacgcg ctcgccgagc aggcgcccgg ctcgctcgag
     4501 ctctaccggc tgctgtcgct gcgcaccctg cccctcggcc tggcgaaggg gacgctttcc
     4561 ccggaggcgg cgcgggcgct cgaggcgctg ctttcccggc ccggcgtatg atcgggccca
     4621 tgcgaagggt ctggctcctc atcgcgctcg tgctcgcgcc cacctgggtc ttcgccgggc
     4681 cggtggtggt gctgacggtg accggggcga tcgggccggc caccgccgac tatgtccacc
     4741 gcggcatcga gcgcgccggc cgcgacggcg ccgagctggt ggtgctgcgg atggacaccc
     4801 ccggcggcct cgacacctcg atgcgcgcca tcatcaagga catcctcgcg tcccccattc
     4861 cggtcgcctc cttcgtggcc ccgggcggtg cgcgggccgc gagcgccggc acctacatcc
     4921 tgtacgcgag ccacgtcgcc gccatggcgc cggcgaccaa cctcggcgcg gcgacgccgg
     4981 tcgcgatcgg gggcggctcc cggggcggtg acgagcccgt gagcgccccg gacaagggca
     5041 aggccgcgaa gccggccccg gccggcgaca cgatgacgcg caagcaggtg aacgatgccg
     5101 ccgcgtacat acgcgggctc gccgagatgc gcgggcgcaa cgccgactgg gccgagcgcg
     5161 cggtgcgcga ggcggtgagc ctgcccgcga gcgaggcgct gaagctcaag gtgatcgacc
     5221 tgatcgcggc cgacgtgccc gacctcctga agcgcctcga cggccgcaag ctcgccacgg
     5281 cggccggcga gaagacgctc gccaccgcgg gcgccgccgt ggtctcgctc gagcccgact
     5341 ggcgcacgcg cttcctgtcg gtcatcaccg acccgagctt cgcctacctc ctggtgctcg
     5401 ccggtatcta cgcgctgttc ttcgagttct cgaacccggg cctcgtgttc cccggcgtgg
     5461 cgggcgcgat ctgcatcgtg gtcgcgctct atgctttcca cctgctgccg gtgagctacg
     5521 ccgggctcgc gctcgtgctg ctcggcatcg cgttcatggc ggcggagctg ttctttcccg
     5581 cgtacggctc gctcggggtc ggcggcgccg tcgcgttcgt caccggctcg gtgatgctga
     5641 tcgacaccga cgtgccgggg ttcagcctgc cgctgccgct catcctcggc gtcgccgcgg
     5701 tgagcgtcgc attcgttttc gtcgtcgtcg gcagcgcgct caaggcgcgc cggcggcccg
     5761 tggtgagcgg cgccgagcag atggtcggca gcaccggcga ggtcctcgcc gactgcgatc
     5821 gcgagggctg ggcgcgcgtg cacagcgaga cctggcgcat ccgctgcgcg acgccgctca
     5881 aggcgggcca gcgcgtgcgc gtcgcccgga tggaaggcct gctgctcgac gtcgtccctg
     5941 atccaggagc ctgaaaaaaa tgttcgctct caactttggc accgtctttc tcgtcgtcgt
     6001 cctcgtggtc gtgttctggt cgttccgcat catgcgcgag tacgagcgct cggtcgtgtt
     6061 ccagctcggg cgcttctggc gcgtgaaggg gccggggctg atcatcgtct ggccggtgct
     6121 gcagcaggcg gtgcgggtcg acctgcgcac catcgtcttc aacgtgccgc cgcaggacgt
     6181 gatctcgcgc gacaacatct cggtcaaggt cgacgcggtg gtgtacttcc gcgtggtcga
     6241 cccgcagaag tcgatcatcc aggtcgccga ttacctcacc gccaccagcc agctcgcgca
     6301 gacgacgctc cgcgcggtgc tcggcaagca cgagctcgac gagatgctcg cggagcgcga
     6361 gaagctgaac atggacatcc agcgcgcgct cgacacgcag accgacgcct ggggcatcaa
     6421 ggtgtcgaac gtcgagatca agcagatcga cctgaacgag acgatggtgc gcgcgatcgc
     6481 gcgccaggcc gaggccgagc gcgaccgccg cgccaaggtg atccacgccg agggcgagct
     6541 gcaggcgtcg gtgaagctgc tcgaggcggc gcagatgctc gcgaagcagc ccgaggcgat
     6601 gcagctgcgc tacctgcaga cgctcaccca gatcgccggc gacaagtcct cgacgatcgt
     6661 cttcccgctg ccgatggaca tcctcacgcc gctcgtcgac gcgatgaaga agcgcaacgc
     6721 ctgaggaaag atcatggacg aatcggccgt ctataccgag cccactttcg tcgaccgcgt
     6781 gcgcgggccg gcgtggatcg cgctcgcggg ctttgcgctc gccgcgctcg cggtcctggc
     6841 cgttctcgcg ggaggctggg gcaaccgcgc cgggtcgtgg gactaccgcg tggcgttcac
     6901 gatcctgcgc tgggcggcgt ggatcgggct cgcgggcgga ctggccgccg gcatcggcgg
     6961 cacgctggcg atttccggcg cgccgccgcg gcgcgtgagc gtgctcgcga tcgccggcgt
     7021 cgtgctcggc gcgctcggct tcggcatccc gtgggcgtac accgcgaagc gcagcgcgcc
     7081 gatcaacgac atcacgaccg atccggcgaa cccgccgcgc tacgtcgaca tcgcgccgct
     7141 gcgcgcgagt gcgccggtca aggccgacta cggcggcgag gagacggcgg cgatccagaa
     7201 gaagacctat cccgatatcg cgcccgcgtt gctcgacgtc tcccgcgagc aggcgtacga
     7261 gcgcgcgctc gcggcggcga aatcgctcgg ctggacgatc gtggcggcgg tgccggcgga
     7321 gggccgcatc gaggccaccg acacgacgct cttcatgggc ttcaccgacg acgtcgcgat
     7381 ccgcgtgcag gccgaaggcg cgggcagccg cgtcgacgtg cgctcgcact cgcgcgtcgg
     7441 gcgcggcgac ttcggcacca acgcgaagcg cgtgcgcgcc ttcctcgcga agctcggccg
     7501 ctagggcttc ttcgccttct tcagcaggcg ctcgcgcacc ttctgcacgt cgacgacgaa
     7561 gcgctcgtgc gtgccgtgcg caaccgcgtc ggcgccgtcg gagaccttcg cctcgaaggt
     7621 gacgcgccgg ccgtccacgc tggcgacgcg gatgtcgagc gtgacctcgg cgccgagcag
     7681 cgccggcccg acgtggtgcc agtgcacctg cgtgccgacc gagtcctcgc ccggatcgag
     7741 gtgcgcgagc aggaaatcgc ggcaggtgta ctcgacgtcg gagatgatgc gcggcgtggc
     7801 gtagacgcgg caatcgtcgc ccatgaagct gatcacgcgc ggctcgtcga cgcggatctt
     7861 cgtcgtcgtc gcgaggcccg gggcgagcga cgccttcacg gcgccgcgtc cttgggccgc
     7921 gcgcgctcgg gcagcggctc gatgccgtcg tggaggaagg cgaggagctt cgcgaggcgc
     7981 gaggaggcga gcctcctcac gaggtccttg tcgcagccgc tccagtcgta gaagccgcgt
     8041 cccgcggaga ggccctcgtg gccgcgcttc accatgttct gcagcatcga gttcggcgcc
     8101 ccgttgtgga agagcttcgg cacgatcgag cgctgcgcgt cggcgtggat ccgcaggccc
     8161 gagatgtcct tctgctggat cagcccgttc aggcacatgc gcggcgcgag catgtgcatc
     8221 gcggcgcggt cgatgtcggc ggccgacgcg accccggcct cgaccaggta gtaggcctcg
     8281 tgcaggatcg cgtgctgcag ccggttgatg aggaagccga cgatcggctt gtacagcacg
     8341 accgtctcct tgccggtgcg ctcgagcgcg gcggccgcgc ggtcgaccgc ctcgcgcggc
     8401 gtcgacggcc cggccatcac ctcgaccacc ggcgtggtgt cggccggcat gaaatagtgg
     8461 atgccgacga agcgcgccgg gtccttcatc ttcgccgaca gcttcaccag gtcgaggccc
     8521 gaggtgccgg tcgcgatcag cacgtccttg cccgggtacg cggcctcgat ctcggcgtac
     8581 accgcctgct tggtcgcgac gtcctcgggg acgaactcga tcaccaggtc cggcgcctcg
     8641 gcgggaaggc ggtcgaccgc gcgcactttc gcgggaaggt cggcgagctc gcgcgcgcgc
     8701 gagctcttca cgacggtgtc gaagccgccc tcggcgaagg tgcgcgcgac gccgcgcccc
     8761 atgacgccgt agccgtagat gagcacgctc gcgatgtcgg cagccatggg gatccttgtc
     8821 agtcgagggg tgcgacgccg acctcgcgcg cgaggcggtc cagggcgtcg cgcagcgcgg
     8881 ccgggaccgg cacgccgtgg cgcgagcgtt cctcgtattt ggcgcggctc tgctcgcccg
     8941 gcagccagac gcgctcgacg cccggcagcc tgcgcgacgc gcgcagctcg cccgcgacgc
     9001 ggtcgacatc gcgcttgaac gcggcgacgt cgccgaaggc cgcgagcgac agcgcgacga
     9061 tcgcgtgccc cgtgttggtg accgaggtgt cgtcggcgtt gaagtcgatc gtctggctgc
     9121 cgagcgcggc gcggttcagc gtcccggcga gcagcccgat catgagcgac aggccgtagc
     9181 ccttgtagcc gccgatcggc aggagcagtc cctcgttcga gcgcttcggg tcggtgagcg
     9241 ggcggccctc gcggtcgatc atccagccct cgggcatcgt ctcgccgcgc tgcgccttcg
     9301 tcttcacctt gccgtaggcg gcgacggtgg tcgccatgtc gagcacgatc ggcgcgcgct
     9361 cgcccgggac cgcgatcgcg atcgggttgg tcgagagcag caggtcgatc ccgccccacg
     9421 gcgccatgtg attggcgttt cccaccgcga ggtagagccc gatcatgtcg tgctcgatcg
     9481 gcatcgccgc gtagagcgac gcaggccccg cgtggttgct cgcgcgcacg ccgacccagg
     9541 cggcgccggc cgtcttcgcc ttggcgatcg cggtctgcgc ggcgaagctc atcacgacgt
     9601 gccccatgcc gttgtcgccg tcgaccagcg ccatgccggc cgcctcgcgc tcgatgcgca
     9661 cgcgcggagc gacgttcacc gcgccgccct ggatccgtct gacatattga ggcagtcgga
     9721 acacgccgtg cccgtccgcc ccgtgcaggt cggcgcgcac catcaggcgg gcgcaggtct
     9781 gcgcgtcggc gcgcgcgagc cccaccttct cgaggaccgt ggacgtgaag gattggaggc
     9841 gttccggcga cacgctcggc gacataaggg ccggattcta tcgcttcggc taccatgcgg
     9901 tagatggcgg gcaacctgca tccggaattg agagtcgggc tcgcgggctc ggccgagctc
     9961 gtggtgggcg aggagcacac cgcgccgcgc gtcggcagcg gccgcgtgca cgtgctcgcg
    10021 acgccggtga tgatcaacct catcgaggcg gccgcgctcg ccgcggtcga gcacctgctc
    10081 gcgcccggct accagtcgct cgggacgctg ctcaacgtgc gccacatcgc ggcgacgccg
    10141 gtcggcatgc gcgtgcgcgc gaccgcggag gtcgtgaaga tcgacggccg caacgtgacg
    10201 ttccgcgtcg aggcgcacga cgagcgcgac ctgatcggcg acggcgcgca cgagcgcgtg
    10261 atcgtgaacg tcgagcgctt cgacaagcgc gtgcaggaga agctgcaggc gatgcgctga
    10321 ccggctccct gcggccggtg gaaccgcggc ttcctttacg ggtcagagac ttatccgcgg
    10381 gacccgggcg cttcgcaggt gcgccacccg gcccacggat gtttcgccgg agaaaaccat
    10441 gacccgattc cgcaccgtat cgttgctcct cgccgtcgcc ggcggcctgc tcgtcgccgc
    10501 gcccgcgttc gccgacgagg tgatcctgaa gagcggcgag cgcatcaccg gccgcatcgt
    10561 cgagaagtcg agcgaggcgc tcgtgctcga gaccgcgagc gcggacaacg tgcgcgtggc
    10621 ctgggccgac atcttctcgc tcaagaccga cgcgccccag gacgcgatgc tcacgtagcc
    10681 tcgcccttcg tctcgcatcc ggaacggcgc ctgcgggcgc cgttttcgtt tccgccgcct
    10741 ttacaaaact ttacccgccg gagaaacggc cgttacggct gccgcgtaga ttgactccat
    10801 cgacggcccg gtgccgtctt ccgaacagga gtcgatcatg aaaggcagtc tcaagcgcgc
    10861 cgcggtcgcg gccctcgtcg gcggcgcgat cgccgtgccg gcgctcgcgc aggaccaaac
    10921 tccccagccg gcgcccggcc agcatcacgg ctggcgccac gcgaaccccg aggcgcgcat
    10981 ggagcacatg gtgacccgca tgttctcctc ggtgaacgcg agcgatcagc agaagacgca
    11041 ggccgtgacc atcgttcgcg ccgcggccgc cgatatccgg ccgctcgagc agcagttgcg
    11101 agacggtcgt cgcgccgcga tgcagttgct tgcggcgccc gccatcgacc gcaacgccct
    11161 cgaggcgcag cgcgtgcagc agtcggccgt gcaggagcag atctcgaagc gcaggacgca
    11221 ggcggtcgcc gatctcgcgg aggtgctcac ccccgaccag cgcgccgctc tcgcgaagcg
    11281 catgtccgag cggttcggcg gacggcgcgc ggggtaacag gcgaacgcaa ctgagcaggt
    11341 cctctccggc catgacatcg gccggcttgc gcccgccgcc cgaaggggcg gcgggccttt
    11401 ttctcggccg cttgttgtac cctagcggcg ccatgacggc ccacatcctg atcatcgagg
    11461 acgacgagcg gctctcggcg atggtggcgg agtacctgca cgagtcgggc ttcctcgtct
    11521 cggcgcgcgc cgagggcggc gaggggctgg cgctgctcga gcgcgagccg ttcgacgcgc
    11581 tgatcctcga cctcatgctg cccgatctcg acggcttcga ggtttgccgg cgcgtgcgcg
    11641 cgcgctccga catcccgatc ctcatgctga ccgcgcgcgg cgaggcgacc gaccgcgtgg
    11701 tcggcctgga gctcggcgcc gacgactacc tgccgaagcc gttcaacccg cgcgagctgc
    11761 tggcgcgcct gcgcgcgatc ctgcggcggc gcaagcccag cggcccgccc gagcccgcgc
    11821 tgcgcttcgg ccggctcgag agcgaccgcg gcgcgcgcgt cgtgcgcctg gacggcgagg
    11881 agaagccgct caccgcctac cagttcgcgc tgctggtcgc gctcgccgag aacgcgggcc
    11941 gcatcatgtc gcgcgagagc ctgatggacc tggtgaaggg cgagccgctc gaggccttcg
    12001 accgctcgat cgacgtgcac atctcgcgca tccgcgccgc gatcgaggac gatcccaagc
    12061 ggccccggcg catcatcacc gtgcgcagcg cgggctacgt gttcgcaaag gtccaggacg
    12121 accgcgagga cgcgtcgtga cgccgccgcg cccgcgccgg ctgtaccggc agttctacgt
    12181 gaccctggtc gcgagcctcg cgctgttcgc agtcatcgcc ggcttcctgt ggcatacgct
    12241 caccgaggcg tcgcccgcgc gccaggcgtt cgagatcctt tccgagctcg cgcaggcgtc
    12301 gctcgccgag gccggcgcgc cgcgcgatgc gcagcaggcg gcgctgaacg atctgcgcgc
    12361 gcgcgtgcgc ggccggttcg cgggcctcgc gctgtatgcc gcggacggcg agcgcatcgc
    12421 cgcggccggc gcgccggtgc cgcgcccggg accgggaagg caggcgggcg gctggattcc
    12481 cggtcccgaa gggcgccacg cctgggcgat cgcgctcccg gacgggcgcg tgctgatcgc
    12541 gcggctgccg cgtccccggc ccaacccgat gctggggctg ttcttcatcc tcggcctgat
    12601 cacgctcgga gtcgcggtcg gcgcctaccc ggtggtgcgt catctcacgc ggcgcctcga
    12661 gcggctgcag gcgagcgtcg aggcgctcgg cgcgggcgat ctgtcggcgc gcgtgccggt
    12721 cgacggcgcc gacgaggtct cggcgctcgc gagcagcttc aaccgcgccg cggagcgcat
    12781 cgagcagctg gtcgcggccc acaagacgat gctcgccaac gcctcgcacg agctgcgctc
    12841 gccgctcgcg cgcatccgca tgggcatcga gatcttacga gccgacgcgc ggcccgagct
    12901 gcgcgaggag ctcgcgcgca acatcgccga gctcgacgcg ctggtcgacg agatcctgct
    12961 cgcgagccgg ctggagacgg tcaagcagcc cgacgcggtc gacacggtcg acctgctcgg
    13021 gctgctggcc gaggaatgcg cgcgcgcgga cgcggcgctc gagggcgaac cggtcaccgt
    13081 gcgcggcgac gcgcggctgc tgcggcgcgc ggcgcgcaac ctgctcgaga acgcgcgccg
    13141 gcacggcggc ggcccggtcg aggcgaccgt gcgcaggacg ccggccgcgg cggcggtgat
    13201 cgaagtgtgc gaccgcggtc ccggcgtgcc cgaggccgag cgcgagcgga ttttcgagcc
    13261 gttctaccgg ccggccggaa cgcgcgagcg ctcagggggc gtcgggctgg ggctctcgct
    13321 cgtgcgccag atcgcgcgca agcacggcgg cgacgcgcgc tgcctgccgc gcgagggcgg
    13381 cggctcgcgc ttcgtgctgg agctggggac ggctacgtag ctcggctcag tcgagttcag
    13441 gtcagccgcc gttcttgcgg ccgaagctgt tgaacagcgc gggcacctgc cgcgggcggt
    13501 cacgccgcgg ggcggccggc tgctgctcct gctgctcctg ctggggttgc tgagccgcgt
    13561 cgtttcggga ttcctcgtcg gcctggctca tgcggcgcgc gtactcgatc tgcgccaggc
    13621 gctcctgctc gtcggcgcgc gggaggcccc gcggctgctg cggacccggc cgtccgcgca
    13681 tcttgccgcg gttggcgtcg cgctgcggtt gccggttctg ctgcggctga tgcgggcgct
    13741 gctgtccgtt gccctgctgc tgccggtggc gccggccttc gcgccggccg cgctgtcctt
    13801 gctgttgtgg cggttgttgc tgtggccgtc gctgttgctg ctgcggctcc cgctgctgct
    13861 gcggctgctg cgccgccgtg cccggctcga atcccggcac cacgcggctc tcgatcctgc
    13921 ggttgatgag gcgctcgatc gcggcgagca acggccggtc ctccggggtc accagcgaga
    13981 tcgcctcgcc ggtctcgccc gcgcggccgg tgcggccgat gcggtgcacg tagtcctcgg
    14041 ggacgttcgg caggtcgtag ttgaccacgt gcggcagcag ctcgatgtcg agcccgcgcg
    14101 cggcgatgtc ggtcgcgacc agcacgcgca gctcgttctc cttgaacgcc ttgagggcgc
    14161 gcgtgcgcgc gttctgcgcc ttgttgccgt ggatcgcgtc ggcctcgatc ttcgagcgca
    14221 cgagctgctg cgcgaggcgg ttggcgccgt gcttggtctt ggtgaacacc agcacctgcg
    14281 tccactcgtt gctcttgatc aggtgctcga gcagcgcgcg cttgcgctcg cggtccaccg
    14341 ggtgcgcgac ctgcgtgatg agctcgatcg gcgcgttgcg ccgcgcgacc tcgaccgtgg
    14401 ccgggtcgtg catgaacgac gccgcgagct tgcggatctc gtcgggaaag gtcgccgaga
    14461 agagcaggtt ctggcgcttc gcgggaagca gcgcgaggat gcgccggatg tccgggatga
    14521 agcccatgtc gagcatgcgg tcggcttcgt cgaggacgag gatctcgatc ctgccgaggt
    14581 cgatcgtgcg ctcctggacg tggtcgagca ggcgccccgg cgtcgcgacg acgatgtcga
    14641 cgccgtcggc gagattgtcg gcctgcgggg tgaagcccac gccgccgaag atcaccaccg
    14701 actcgagcgc gaggtggcgg ccgtagtcgc gcacgctctg ctgcacctgc gcggcgagct
    14761 cgcgggtggg cacgaggacc agcgcccgga ccggccgcgg accgggcgcg gcgcgcgtcg
    14821 cgttcagccg ctgcagcatc ggcagcgtga agccggcggt cttgccggtg ccggtctgcg
    14881 ccgcggcgag caggtcgcgg ccggaaagga cgaccgggat cgcctgggcc tggatgggag
    14941 tgggttcggt atagccctgc tcggcgaccg ccttgagcag ctcggccgac aggccgaggt
    15001 tctgaaatgt cgagctaatg agcaatctcc tgaatcggcc ctggaaggat gaccttccac
    15061 accggttcag gacgacacgt tcgttttaag gattcggggg caaagccacg aaagggcagg
    15121 ccgctaaccg gatgaagcaa ccagaaaccc aagtatcagt atacacgaac ctgccgggga
    15181 cggctcactt gtgccagccg gcgatatcgc cgagctgcac gttgtagccg acgttctgcg
    15241 gaccgagcgc ggcgagcgag gtcttcgcgg actcctggaa cgccgccgcc agctcgaagc
    15301 tcagcttcgc gagcaccgcg gcttcgggct tgatggtggc cgcgttgtcc acgcgcagga
    15361 aggcgcggcc gaccagctcg acgtagatgg ccgcggccag ctcggcgacg gattgtctcg
    15421 cttcgtcttc cttggcggag ctcatggatg cggccttttc gggatctgcc cccatgctac
    15481 gccgaatccg gacctgcgct agactatcgg cgacccgccc gccgtccggc gagcacgacc
    15541 acccacccct cgtaccaccg cacgaaagga acccccatga agctttatta cgctccgggc
    15601 gcctgctcga tggcgccgca catcgtcctg cgcgaggcgg gctaccagtt cgacctcgag
    15661 aaggtcgaca ttccgaacaa gaagacggcc tcgggcgacg acttctggaa ggtcaacccc
    15721 aagggctatg tccccgcgct caggctcgac gacgggcagg tgctcaccga ggcgcaggtg
    15781 atcgtgcagt acctcgccga tcacaagcag gacgcgggcc tcgcgccgcg cggcggcacg
    15841 atggagcgct accgcctgat ggagatgctc aacttcaccg ccgccgaggt ccacaagcag
    15901 ctcggcgcgc tgttcaaccc gaagatgacg cccgaggcga aggagatcca gctcggcgtg
    15961 atcgaccgtc gcctcaccgc gctcgacaag atgctcgcgg gcaagcaata cgcgaccggc
    16021 gacaagttca cggtcgccga cgcgtatctc ttcaccgtgc tcaactggac caacttccac
    16081 aagatcgaca cgtcgaagtg gccgaacatc caggcgttca tgaagcgcgt ggccgcccgg
    16141 cccaaggtgc aggagacgct gaaggccgag ggcctgacgc agtaatcagg agcccgtgcg
    16201 cggcgctatg cgccggtgta gaccgcgccg tgacgcaccg agtcgcggcg cacctcgacg
    16261 cggcacagcc ccgggaaggc gggcttgagc cggccccaga tccagatcgc gatgtgctcg
    16321 aggctgggga cctcgagccc gcccacctcg ttcaggaagc ggtgatcgag cgactcgcgc
    16381 agcccggcgc aggcgcgctc gagctccccg aggtcggcga cgaagcccgt gagcgcgtcg
    16441 ggcgtgcccg ccaccacgac gcgcgcggtg aacgagtgcc cgtgcacgcc gtggtagcgg
    16501 tgcccttcgg gcgcgcccgg gaagcggtgc gcggcgtcga agctgaactc ctggtagatc
    16561 tccacgtcgt cctctagcgg atgccgatga gcttgtgcgt ctggaggctg agcctccacc
    16621 tcgggttgcg catgcagtag gcgatcgcgc gctcggtgtt ctcgcgcttc tccggcccgt
    16681 ccatgggctg cagcaggaag tgcccgaagc tccagccctc gaagcgctcc ggcggcgcgc
    16741 cttcctgcgg gaacacgagc ttcagctcgt cgccgcggcg gaccttcacc tcggtgccgg
    16801 ccttcgggct gacgcagatc cagtcgactc ccggggcgag ctcgagcgtg ccgttcgtct
    16861 cgatcgcgat ctggaacccg cgcgcgtgca gcgcgcgcac gagcggctcg tcgagctgca
    16921 gcgcgggctc gccgcccgtg agcaccgtga agcgcgcgcc cgctcccggc cagagcgagg
    16981 cgatgcggtc cgcgagcgcg ccggcgtcgt cgaacttgcc gccgcccggg ccgtcggtgc
    17041 cgacgaactc ggtgtcgcag aacctgcaga cggcgcccgc gcggtcggcc tcccggccgc
    17101 tccagagatt gcacccggcg aagcggcaga agaccgcggg acgaccggcg ttcgcgccct
    17161 cgccctgcag cgtgtagaag atctccttca ccgagtaggc catgaggctt ggatcgctag
    17221 cggcacacgc ggttgcgccc gccgcgcttc gcgcggtaga gcgcggcatc ggccgcccgg
    17281 atcacctgcc cgggggcggt cagcatgccg tcgcgctcgg cgacgccgat gctgatcgtg
    17341 accgagagcg tgcggtcggc ctgcgaaggc gttcccggga tgcgcatcct cagcccggtc
    17401 tcgggatcct tcgggcggtc gagcccgcgc accgccatgc ggtagcgctc gatcgagtcg
    17461 cgcatcgcct ccaggtgcgg cagcgcctcg gagagcgggt gctcgccgaa gacgaccgcg
    17521 aactcctcgc cgccgtagcg gaacgccttg ccgcccccgt cgatctccgc gaggcgcgag
    17581 gcgaccagct tcagcacctg gtcgccgacg tcgtgaccgt gcgtgtcgtt gaacttcttg
    17641 aaatggtcca cgtccaccat cgccaccgta tacaccggtc cgaggccgga gaggcgctcc
    17701 tcgagcgcgc gccggcccgg gaggccggtc agctcgtcga cgaaggccag ccggtgcgac
    17761 tcctgcagga tcgacacgag cagcacgatg cccgcggccg acatgaagat cgggaacacg
    17821 cccggcgagc gcgcccactc gcaggcgatg aagaacgcga cgagcgcgcc cgcgaggccg
    17881 gcgtcgatcg gccgcacctc gactccggcg cgcggccagg cgcgcgcgag cgcggcgacc
    17941 agggccgcgg cgaacgcgat gctcccggcg atgggggccg gcggggacct gagcagccag
    18001 tggtcgagca cgccgtgcca ggcgacgccc gagaggctcg agcgtcccgc gctcgcgatc
    18061 caggcgacga gcagcacctc cgcgagcgcg agcagcagcc agcggtagtt gcggtgctgg
    18121 accacgccgc gctcgggaaa gagcagggcg atcagcacgt tcagcggaac cagcaccgag
    18181 agcgcggtga acacggcgcg cgcggtgaag ctgtcggcgc cgaactcgag cgcgatgcgg
    18241 tagccggcgt acgcggcgaa cagcgaggcg agcgcgacaa aggcccggcc gcggttgaac
    18301 cacacggcga tgatcgcgcc cagcagcagc acgaaatacg ggccgagcac ggtcaggccg
    18361 gacagcgagt cgggcagcga cccgatccac acgatcacgg cggacgctac caggaccacg
    18421 accggcgcca gcaccgggcg caggtgttcg agcaggagtt tgggcctcaa gacaggcgca
    18481 tgatagcgag cgcggtcagc ctgcgctacg cggccttgcg cagcagccgc tcgcattcct
    18541 gcgccgacgc gggcgacgcg aaccagaagc cctggatctc gtcgcatccg tgcgcgaaca
    18601 gcaggtcgag ctgctcgcgc gtctcgacgc cctcggcgat gacgcggagc ttgaggctgt
    18661 gcgcgagccc gatgatggcg aaggtgatcg ccgcgtcctc ggggtcgatc gtgacgtcgc
    18721 ggatgaaggc gcggtcgatc ttgagcgcgt ccagcgggaa gcgcttcagg taggcgaggc
    18781 tcgagtagcc ctggccgaag tcgtcaaccg acaccgccac gcccgactcc ttgagcgcct
    18841 tcagcgtgcg cgccgcgctc tcgggatcct tcatcagcag cgactcggtc agctcgaact
    18901 ggatgagcga cgcgtcgacc tcggtctcgt acaggatgcg gcgcaccgtg gcgtcgaggt
    18961 tcttgtgctg gaactgccgc gccgacaggt tcacggcgac cggcgggacg tcgaggccca
    19021 tgttcttcca gaccttcacc tggcggcaca cctcgcgcat cgcccattcg ccggccggga
    19081 tgatcagcgc ggtctcctcg agcaccggga tgaactcgag cggcgagacg atgccgcgct
    19141 cgggatgctg ccagcgcatc agcgcctcga agccgctgat cgcgccggtt gcgaggtcca
    19201 tcttcggctg gtagtgcatg aggaattcgc cgcgctccag cgcgcgccgc agcgacgcct
    19261 ccatctgcac gcgcgcgagc gcgcgctcgt tcatctcgcg cgtgaagtac tggtgggtgt
    19321 tgcggccctg ctccttcgcg cggtacatcg ccgtgtcggc gttcatcacg agcgattcgg
    19381 gcgcctcgcc gtcggtggga tagatcgtga tgccgacgct cgccgagatg aaggtctcct
    19441 gcccgtcgag ctggaacggc tgcgacagcg cgtcgatgat cttctgcgcg accaggctcg
    19501 cgtcggcggg gcggccgagg tcggtgagga tggcgccgaa ctcgtcgccg ccgaagcgcc
    19561 cgacggtgtc gccgctgcgc acgcagtcgg cgagccgcgt ggccgcctcc gcgagcagcg
    19621 tgtcgccggc gcggtgcccg agcgtgtcgt tcaccagctt gaagcggtcg aggtcgatga
    19681 agagcaccgc gatcgcgcgc gcggcgcgct tcgcctgcgc gatgctctgc accaggcggt
    19741 cgtggaagag gtagcggttg ggcagcccgg tgagcacgtc gaactgcgcg aggtgcgcga
    19801 gctgctcctc ggcgctcttg cggtcgatcg cgacggcgag gatgttggcg acgctctgca
    19861 ggaagctcgc gtcgtcgcgg ctgaagcggt gcacggtgaa gcggtgcacg ccgagaacgc
    19921 cataggcgcc ctggctgccc atgatgggca cctgcatgcc cgagcggatg ccgaactgca
    19981 tcagcgccga cccggggaag cgcgtctcgg ccgagtggtc ctcgacgacg accggctcgc
    20041 gcgaggcgag cacgtgcgcg agcatgccgc ccggccggac ctgcaccacg cgacggccca
    20101 cccattcggg cggccagccg gccgcggcgc ggtacgtgag cgccttgccg tcggcgttca
    20161 gctcgagcac ctcgcagtag tcgaccttga gcgtgaccga gaccagctcg acggcgtgat
    20221 tgagcacgtc ctcgaggtcg gcgctcgcga gcgcctgctg gccgaactcg gcgatgaggc
    20281 gctgctgcag cgcctggctg cgcaccgtct cctcggtctc cttgcgccgc gtgatgtcgg
    20341 cgatcgcgcc gatcatgcgc accggcctgc cgttgtcgtc gtagacgacg tggccgcggt
    20401 cgaagacgtg cgcgtaggtg ccgtcggcgc ggcggaaccg gtattcgtcg ctccagttcg
    20461 cgccgcccga gtcgagcagc tgcgcgagcc cgctctcgac ccggtcgcgg tcgtccgggt
    20521 ggatgttgcc gtaccagaag cggttgctcg ggtcgacctg ctcgcgcccg tagccgagca
    20581 ccttggaaag gttgtcgttc caccagactt cgtcggtgat caggttccag tcccagatga
    20641 tgtcgttggt ggcccgcacg gcgaggttga agcgctcgtt gctccggcgc agcgcctcct
    20701 ccgcggcgat gcgctcgctc gtgtcgcgcg agatcacgac cacgatccag cggtcggccg
    20761 agcgcagcac gcggcgtgtc gactcgaacg gcagcagcga gccgtccttg cggcggtaat
    20821 ggctgcgcat gccgctgtgc atctggccgg tgcggatcat ctcgtcgtag gcgcgctcga
    20881 gcgagtcgcg ggtgatcggc agcaggtcct gcggtccgat ctggagcagg tcctcgcgcg
    20941 agtagccgag cgagcggcac gcggtctcgt tcatgtcgat gaagcgcatg cgctcgcggt
    21001 cgatcaggaa gatcatgtcg cccgacaggt ccatcgaggc gcggaagcgg cgcagctcgt
    21061 cctcgcggcg gaccgactcg gtcacgtcct tgccggtgcc gcggtagccg cggaacgcgc
    21121 cgcgctcgtc gaacaccggc tcgccgctca cgctcagcca gcgcagcgtg cccgtctggt
    21181 cgagcgccgg gcgcacgaaa tcccggaacg gccggtgcgc ctcgagcgtc tcgcggtgcg
    21241 cgatccagtc gacgtcgcgc tcggcggtgt tgtcgagctc ccagcgggtc ttgcccagca
    21301 ggcgcgcggc gggcgagggc atgttgccgg cgagcgcggt gaagcggaac tcggcgtcct
    21361 gctcccagta ccagtccgac gacagctcgg tcaggctgcg gaagcgctcc tcgctctcgc
    21421 gcagcacctt ctcggcgcgc ttgcgatgga cgaactgccc gatctggctg ccgatcacgc
    21481 gcgcggcctc gagcaggcgc tcgtcgggct cgcgcaccgt ccggctgctg aagtcgaata
    21541 cgccgatgac cttgccttcg gacacgaccg ggaacaggaa caccccgtgc acgcccaccg
    21601 cggtgacgag gtcgggccgc ttcacgcgcg cgtcgcgggt ggcgtcgggt atccagaggg
    21661 gcatgcccga tccccacacg acgccgacca ggccctcgcc gcggcggaac gtgccgacgc
    21721 tcgaggcctt gtcgagcgcc tcgatgctgc tgcccgggac gctgtacgac tccgcgaacg
    21781 ccatggcgtc cttcgattcg tcccacgtcc agtagacgcc gcactcgagg ctctgcgact
    21841 cgcacaccgc gcccatgacc gtcttcaggg ccgtcgagac attgtcggcc tccgcgagcg
    21901 tgcgcccgac cgtgtgctcg agccgcagca ccttctcggc gcgcttgcgc tcggtgatgt
    21961 cgcgcccgct gccgcgatag ccgcggaacc cgccgtcgac gtcgaagatc gggctgccgc
    22021 tgatgctgat gaagtgctcg tgtcccgagg cgtcgatcca gtacatctcg agatcgtgaa
    22081 aggggcggtg cgcctcgagc tcggcgcggt gctcctccca ggtgcagccg accggctgtg
    22141 cgccggtctc ccagcgcgtc ttccccatca ggaagtcggg cggcatggtg cccttgccca
    22201 cgagcacgcc ggcgatggtc gtgaagcggt attgcgcgtc ctgctcccag taccagtccg
    22261 acgacagcgc ggtcaggctg cggaagcgct cctagctctc gcgcagcgcc tgctcgatgt
    22321 ccttgcgctt caggaactgg ccgacctggc tgccgatgac gcgcaccgcc tgcagcagcc
    22381 ggtcgtcggg cgcgcgcacg cgggcgcacg agatgccgat cacgccgatc gtcctcgcct
    22441 cggcggtgac gggaaacacg aaggccgcgc ggatcccgcc tttggcgaac gaggcgcggt
    22501 acatggcgcg cgggtcgctg ccgacgtcgg cgacccacag cggctcgccg gtctgcagga
    22561 ccttgccggc gaagccttcg ccggggcgga acacgaaccc gcgcgacgcc tcgatcagct
    22621 cctggatgcg cggaatgggc gggccccagg cgtcgccgaa gcgcagcacg ccgtccgcct
    22681 cgtcgacccg ccagtagcgc ccgcagtccc agacctcggt ctcgcacagc gcgcggatga
    22741 cggcgcgcag gcccgtcgac gcgctctcgg cctccgcgag gcttcgcgcg accgagtgct
    22801 cgagccgcag cagctcctcc tcgcgcttgc gcgcggtgat gtccttgccg acgccgcggt
    22861 agccgcggaa tttgcccgcg tcgtcgaaga tcggctcgcc gctgatgctc gcgtagcagg
    22921 ggttgccttc ctggtcgggg cggcagatct cgaagtcgga gaacggctgg tgcgcctcga
    22981 ggagggcgcg gtgcgcctcc cagtcggcgt gcgtcatgtt gagcgtcggg agctcccagc
    23041 ggcgcttgcc gatgtgcgcc gcggccgcca cgcggcccga gcggccgagg agcgcgtcgg
    23101 acatcttggt gaagcggaaa ttctcgtcct gctcccagta ccagtccgac gagagcttcg
    23161 acaggctgcg gaagcgctcc tcgctgtcgc gcagcgcgtc ctccgcgcgg ctgcgctcga
    23221 gcacgatgcc ggcgatgttg agcacgcggt cgatcagcgc caggtcgcgc tcggagggcg
    23281 cgcgcggctg gcggtagtac tgcgcgaacg tgccgagcac cttcttctcg cccgacagga
    23341 tcggcaccga ccagcaggcg cgcagtccga gccgctccgc gagcgcgcgg cgctcgatcc
    23401 acagcgggtc ggtcgagagg tcctcgacga tgacgcgctc ggcgctgaac gccgcgcggc
    23461 tgcaggggct ctcgcccggc ccgatcgcga tcccgtccac cgcctcgcgc cacgcggcgg
    23521 ggaggctcgg cccggcgccg tggcgcaggc gcctgccgtc ccggtcgacg aggaggaccg
    23581 agcacagcag cccgtcgacc tcggcctcga ccgagcgcgt gagcgcctcc agcaccgcgt
    23641 cgaccgggcg cccgcgcgcg atcatctcga ggatcgcctt ctcgcgcgcg aggaactgct
    23701 cggaccaggc gggcgcgccg ccgtcggcgc gatgcggctg cgccctgcgc gcgcgcttgg
    23761 cgttcaggac ggctcgtcgt cgggtcgagg cgctttccac gatggcgatt ggaacggtag
    23821 gaagccgatt tttcttccgg aaacagcgca gtatggtgca ttttgcccgg ttcaaccagc
    23881 gacgctccgc gtccggcggg acccggggcg atgtgtccgg ggtgcgtcgt gacctacatc
    23941 acgccggggt gcggttccgc cggtcaggcg gcgggcgcgg gctccaggtc cgcgggcttg
    24001 cgcgtcgaga ggaattccgc gagcgcgcgc gcggtgtgcg acgtgcgctt cgggtcgacg
    24061 atgcgcgcgg caagccgcgc cggcggtccc tgctcgacga tgcggccgcc cgcttcgccg
    24121 ccctcggggc cgagatcgac gatccagtcg gcgtcggcga tcacgtcgag gttgtgctcg
    24181 atcaccacca cggtgttgcc cgcgtcgacc agccggtgca gcacgcggat cagcttctcg
    24241 acgtcggcca tgtgcaggcc caccgtcggc tcgtcgagca cgtagagcgt gtgcaccgcc
    24301 gaggcgatgc ggccgcgccg gccgtcggcg ggcacggcgg tgccggcctt ggcgagctcg
    24361 gcgaccaatt tgatccgctg cgcctcgccg cccgagagcg tcgggctctg ctggccgagg
    24421 gtgaggtagc cgaggccgac gtcctggagc agccgcagcg cgcggtgcgt cgacgggtgc
    24481 gcggcgaaga agtcgaccgc ctcctcgacg ctcatcgcca ggacctcgcc gatggtcttg
    24541 ccgcgccagc ggaccgccag ggtctcggcg ttgaagcgcg cgccgccgca ggcctcgcag
    24601 gtcactttca cgtcgggcag gaagctcatc tcgatcgtct tcacgccctg gccctcgcag
    24661 ctctcgcagc ggccgcccgc ggtgttgaac gagaagcgcg acgcggtcca gccgcgcatg
    24721 cgcgcttccg ccgtctcggc gaagatgcgc cggatcgaat cccagaagcc gatgtaggtc
    24781 gcggggcacg agcgcggcgt cttgccgatc ggcgtctggt cgacctcgag cacgcggccg
    24841 acgctctccc agccgcggat ctcgcggcag cccgcgggcg cggcggcgcg cttgcgccgc
    24901 ttcgcgccga gcagcgcggc gaggttggcg tacaggatgt cgcgcgcgag cgtcgacttg
    24961 cccgagcccg agacgccggt gaccgagacg aggcgcgcga gcggcaggcg cacgtcgacg
    25021 ttcttgagat tgtgcagcgt cgcgcccagg atctcgaccg cgggcgcgtc gcggccggtc
    25081 gcgcgcggcg ggtgcagcgg gtgctcgagc gggtgcgcga ggcagcgccc cgtcgccgag
    25141 gcggggttgc gcaccaggtc gcgcgcggtg ccctgcgcgg tgacgcggcc gccgtgcttg
    25201 ccggcgccgg ggccgaggtc gatgacgtgg cccgcgcgcc ggatcgtgtc ctcgtcgtgc
    25261 tcgaccacca ccagcgagtt gcccttcgcc tcgagcttct ccagcacgtc gagcaggatg
    25321 cggttgtcgc gatggtgcag gccgatggtc ggctcgtcca ggatgtagca cacgccgcgc
    25381 aggttggagc cgagctgcgc cgcgaggcgg atgcgctgcg cctcgccgcc cgagagcgtc
    25441 ggcgcggcgc ggtcgagcgt gaggtagccg aggccgactt cctcgaggaa ctcgaggcgg
    25501 ctcttgatct cggtgacgac gtcgcgcgcg atctcggtgt cgcggccggt gagcgccagc
    25561 gactcgaccc acttgcgcgc gtcgctgacc gaccactgcg
//
LOCUS       DATDMW010000307         5145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1337914, whole genome shotgun sequence.
ACCESSION   DATDMW010000307 DATDMW010000000
VERSION     DATDMW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337914"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="thrC"
                     /locus_tag="VI319_13030"
     CDS             complement(<1..1048)
                     /gene="thrC"
                     /locus_tag="VI319_13030"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY6864813.1"
                     /translation="MLYVSTRGGSPPQPFTSILLEGLAPDGGLYLPEAFPLFDPAGLA
                     AMRRMSYRELAFTVLSKFMDDIPAADLRAIVERTYTAATFRTGDITPLRTLEPGLQVL
                     GLSEGPSLAFKDVALQLVGNLFEYALAKRGARLNVLGATSGDTGSSAEYALRGKKNVA
                     VFMLSPHGRMTPFQAAQMYSLQDANIHNIAVRGVFDDCQDLVKAVNADAAFKSRHAIG
                     AVNSINWARVAAQAVYYFKGYFAASAKNGEPVSFSVPSGNFGNVYAGFVARQMGLPIR
                     RLIVATNENDVLDEFFRTGRYRVLKAAEVRATSSPSMDISKSSNFERYVCDLLEREYE
                     KVRALWSKLDRDGAF"
     gene            complement(1048..2364)
                     /locus_tag="VI319_13035"
     CDS             complement(1048..2364)
                     /locus_tag="VI319_13035"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY6864814.1"
                     /translation="MKPIKVGLLGIGTVGGGTWTVLSRNQEEISRRAGRAIEISVVAD
                     KETDKAKKLTAGRARVTGDAFEVVRSPDVDIVIELIGGYGVARDLVLAAIENGKHVVT
                     ANKALLATHGNEIFKAAQKKGVMVAFEGAVAGGVPIIKALREGLTANRIVWIAGIING
                     TSNFILSEMRDKGLAFDAVLKEAQARGYAEADPTFDIEGVDAAHKLTIMSAIAFGIPM
                     QFDKCYTEGISKLTQADIRYAEQLGYRIKLLGITKRVKEGIELRVHPTLIPTKRLIAN
                     VEGVMNAVLVKGDAVGPTMYYGAGAGAEPTASAVVADLVDITRLHTADPDHRVPHLAF
                     QPDRMSDVPVLPMDEVVTSYYLRMRVLDRPGVLADVTRILADASISIDAMIQREPGEG
                     EEQVDIIMLTHPAREKNVNAAMAKIEALPVVTGKVTRIRLEELGRS"
     gene            complement(2361..2804)
                     /locus_tag="VI319_13040"
     CDS             complement(2361..2804)
                     /locus_tag="VI319_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6864815.1"
                     /translation="MIEIVDVTDDRGVVAARDWLRKAEAVHRQLRPDMPPEYEAKMER
                     VFAGGGRMCVAVDGAKVAGVAVYRVYENTWAGRQLYVDDLVTDEATRSKGVGRTLLGH
                     LERKARAEGLDNFALDSGTHRQQAHKFYFREGMVVTSFHFAKKLS"
     gene            complement(2801..4072)
                     /locus_tag="VI319_13045"
     CDS             complement(2801..4072)
                     /locus_tag="VI319_13045"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY6864816.1"
                     /translation="MSSVPLRPVSKPVPPALAEVRKSTKLADVCYDIRGPVLERAKQM
                     EEEGHRILKLNIGNPAPFGFDAPDEVRHDVMVNLGNASGYSDSKGLFAARKAIMQYSQ
                     EKKIPGVELDDIIVGNGVSELIVMAMQALLNNGDEVLVPAPDYPLWTAAVSLSGGKPV
                     HYLCDEQSDWLPDVADVRAKITERTRALVVINPNNPTGALYPEALLRELVAIAREHGL
                     IVYADEIYDKVLYDGAKHTSIASLADDLLFVTFNGLSKNYRACGYRAGWMVLSGEKRH
                     ARDYLEGLNILASMRLCANVPGQYAIQTALGGYQSINDLVAPGGRLARQRDLAHSLIT
                     QIPGVSCVKPKAALYLFPRLDLQMYPVADDQKLVLELLEAEKVLLVQGTGFNWPKPDH
                     FRIVFLPNSDDLTEAIGRLDRFLEHTRKRRA"
     gene            4157..4528
                     /locus_tag="VI319_13050"
     CDS             4157..4528
                     /locus_tag="VI319_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEY6864817.1"
                     /translation="MKLHLTGSGALNLFTAYGEGYVAVNGRRYGKSLIVLPDRIVEDW
                     GATSFDALSDAHTAALAGLDREVVLLGTGKLLRFPRPELMRPLAQERVGVEVMDLQAA
                     CRTYNILASEGRRVAAALLFD"
     gene            4529..>5145
                     /locus_tag="VI319_13055"
     CDS             4529..>5145
                     /locus_tag="VI319_13055"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY6864818.1"
                     /translation="MVAGPRGWVLIAVATAIWFSALGFRTLVKPDEGRYAEIAREMNA
                     SGDGITPRLNGIKYFEKPPLQYWATAAAFTIFGENDWTARLWAGLTGFLGVLIAGALA
                     WRLYGRLAGVLTATMLATSVMYFVIGHLNTLDMGTAVFLEAALAAFIFAQSAPPASRA
                     ERRWMLAAWVAMALATLSKGLIGLVFPVLTLIAYSIVERDLRPWKR"
BASE COUNT          757 a         1746 c         1761 g          881 t
ORIGIN      
        1 cgaacgcccc gtcgcggtcg agcttcgacc acagcgccct caccttctcg tattcgcgct
       61 cgagcaggtc gcagacgtag cgctcgaagt tcgaggactt ggagatgtcc atcgacgggc
      121 tcgaggtcgc gcgcacctcc gccgccttga gcactcggta gcggccggtg cggaagaact
      181 cgtcgagcac gtcgttctcg ttggtcgcga cgatcagccg gcggatcggc agccccatct
      241 gcctcgcgac gaacccggcg tagacgttgc cgaagttgcc cgagggcacg gagaacgaga
      301 ccggctcgcc gtttttcgcc gacgcggcga agtagccctt gaagtagtac accgcctgcg
      361 cggcgacgcg cgcccagttg atcgagttca ccgcgccgat cgcgtgcctc gacttgaacg
      421 cggcgtccgc gttcaccgcc ttcaccaggt cctggcaatc gtcgaacacg ccgcggacgg
      481 cgatgttgtg gatgttcgcg tcctgcagcg agtacatctg cgccgcctga aacggggtca
      541 tgcggccgtg cggagacagc atgaagaccg cgacgttctt ctttccccgg agcgcgtact
      601 cggcggacga accggtgtcc cccgacgtcg ccccgagaac gttgaggcgc gcgccgcgct
      661 tcgcaagggc gtactcgaag aggttcccga ccagctgcag cgcgacgtcc ttgaacgcga
      721 ggctgggacc ttcggacagg ccgaggacct gaagcccggg ctcgagcgtc ctgagcggtg
      781 tgatgtcgcc ggtgcggaag gtcgccgctg tatacgtacg ctcgacaatg gcgcgcaggt
      841 cggcggcggg aatgtcgtcc atgaacttcg acagcaccgt gaaggcgagc tcgcggtagc
      901 tcatgcgccg catcgccgcg aggccggccg ggtcgaagag cgggaaggcc tcgggaagat
      961 acagcccgcc gtcgggagcg aggccttcga ggaggatcga ggtgaagggc tggggcgggg
     1021 agccgccgcg ggtcgagacg tacagcacta cgagcggccg agctcttcga gccggatgcg
     1081 cgtcaccttg ccggtcacca ccggcagcgc ctcgatcttc gccatcgcgg cgttcacgtt
     1141 cttctcgcgc gccgggtgcg tgagcatgat gatgtcgacc tgctcctcgc cctcgcccgg
     1201 ctcgcgctgg atcatcgcgt cgatcgagat cgaggcgtcg gcgaggatgc gcgtgacgtc
     1261 ggcgagcacg cccggccggt cgagcacgcg catgcgcagg tagtacgagg tcaccacctc
     1321 gtccatcggc agcaccggca cgtcggacat gcggtcgggc tggaacgcga ggtgcggcac
     1381 gcggtgatcc gggtcggcgg tgtgcaggcg cgtgatgtcg acgaggtcgg cgacgaccgc
     1441 gctcgcggtg ggctcggcgc cggcgcccgc gccgtagtac atcgtcgggc cgacggcgtc
     1501 gcccttcacc agcaccgcgt tcatcacgcc ctcgacgttg gcgatcaggc gcttcgtcgg
     1561 gatcagcgtc gggtgcacgc gcagctcgat gccctctttc acgcgcttgg tgatcccgag
     1621 cagcttgatg cggtagccga gctgctcggc gtagcggatg tcggcctgcg tcagcttcga
     1681 gatgccttcc gtgtagcact tgtcgaactg catcgggatg ccgaaggcga tcgccgacat
     1741 gatcgtgagc ttgtgcgcgg cgtcgacgcc ctcgatgtcg aaggtcgggt cggcctcggc
     1801 gtagccgcgc gcctgcgcct ccttgagcac cgcgtcgaag gcgagaccct tgtcgcgcat
     1861 ctcggagagg atgaagttcg acgtaccgtt gatgatgccg gcgatccaca cgatccggtt
     1921 cgccgtgagg ccctcgcgca gcgccttgat gatcggcacg ccgcccgcga ccgcgccctc
     1981 gaacgcgacc atcacgccct tcttctgcgc cgccttgaag atctcgttgc cgtgcgtggc
     2041 gagcagcgcc ttgttcgccg tcaccacgtg cttgccgttc tcgatcgcgg cgagcaccag
     2101 gtccctggcg acgccgtagc cgccgatcag ctcgatcacg atgtcgacgt cggggctcct
     2161 cacgacctcg aaggcgtctc cggtgacgcg cgcgcggccg gcggtgagct ttttcgcctt
     2221 gtcggtctcc ttgtcggcga ccacactgat ctcgatcgcg cgccctgcgc ggcgcgagat
     2281 ctcctcctgg ttgcgcgaca gcaccgtcca cgtgccgccg ccgaccgtgc cgatgccgag
     2341 cagcccgacc ttgatcggct tcatgacagc ttcttcgcga agtggaacga cgtcaccacc
     2401 atgccttcgc ggaaatagaa cttgtgcgcc tgctgccggt gcgtgcccga gtcgagcgcg
     2461 aagttgtcga gcccctcggc gcgcgccttg cgctcgagat gaccgagcag cgtgcggccc
     2521 acgcccttcg agcgcgtcgc ctcgtcggtg acgagatcgt cgacatagag ctgccgcccg
     2581 gcccaggtgt tctcgtagac gcggtacacc gcgacgccgg cgaccttcgc cccgtcgacc
     2641 gcgacgcaca tgcgcccgcc gcccgcgaag acgcgctcca tcttcgcctc gtactcgggc
     2701 ggcatgtccg ggcgcagctg gcggtggacg gcctcggcct tgcgcagcca gtcgcgtgcc
     2761 gcgacgacgc cgcggtcgtc ggtgacgtcg acgatctcga tcatgcgcgc cgcttgcggg
     2821 tgtgctcgag gaagcggtcg aggcggccga tcgcctcggt caggtcgtcc gagttcggca
     2881 ggaacacgat gcggaaatgg tcgggcttcg gccagttgaa gccggtcccc tgcaccagca
     2941 gcaccttctc ggcctcgagc agctcgagca cgagcttctg gtcgtccgcg accgggtaca
     3001 tctgcaagtc gagccgcggg aacaggtaca gcgccgcctt cggcttcacg cacgacacgc
     3061 ccgggatctg cgtgatcagc gagtgcgcga ggtcgcgctg gcgcgcaagg cgcccgccgg
     3121 gcgcaacgag gtcgttgatg ctctgatagc cgccgagcgc cgtctggatc gcgtactgcc
     3181 cggggacgtt cgcgcacagg cgcatcgagg cgaggatgtt cagcccctcg aggtagtcgc
     3241 gcgcgtggcg cttctcgccc gagagcacca tccagccggc gcggtagccg caggcgcgat
     3301 agttcttcga cagcccgttg aaggtgacga acagcaggtc gtcggcgagc gaggcgatcg
     3361 acgtgtgctt cgcgccgtcg tagagcacct tgtcgtaaat ctcgtcggcg tagacgatca
     3421 ggccgtgctc gcgcgcgatc gcgaccagct cgcgcagcag cgcctcggga tagagcgcgc
     3481 cggtcgggtt gttcgggttg atgacgacga gcgcgcgcgt gcgctcggtg atcttggcgc
     3541 ggacgtcggc gacgtcgggc agccagtccg actgctcgtc gcagaggtaa tgcacgggct
     3601 tgccgcccga gaggctcacc gccgcggtcc acagcgggta gtcgggcgcg ggcaccagca
     3661 cttcgtcgcc gttgttgagc agcgcctgca tcgccatcac gatcagctcg gagacgccgt
     3721 tgccgacgat gatgtcgtcg agctcgacgc ccgggatctt cttctcctgc gagtactgca
     3781 tgatcgcctt gcgcgccgcg aacaggccct tcgagtcgga gtagcccgac gcgttgccga
     3841 ggttcaccat cacgtcgtgg cgcacctcgt cgggcgcgtc gaagccgaag ggcgcgggat
     3901 tgccgatgtt cagcttgagg atgcggtgcc cctcctcctc catctgcttg gcgcgctcga
     3961 gcaccgggcc gcggatgtcg tagcagacgt cggcgagctt ggtcgatttg cgcacctcgg
     4021 cgagcgccgg cggaacgggc ttcgaaaccg gacgtagcgg aacgctgctc atcttgcctc
     4081 ctgcacggtg ctgcggcgca aaagttataa tgttactcga tacttatggc ttcggcagcc
     4141 gcagcctaac cttcccttga agctgcatct taccggttcg ggcgccctca atctgttcac
     4201 cgcatacggc gaaggctacg tcgcggtcaa cggacggcga tatggaaaga gcctgatcgt
     4261 gctccccgac cgcatcgtcg aggactgggg cgcgacgagc ttcgacgcgc tgtccgacgc
     4321 gcacacggcc gcgctcgcgg gactcgaccg cgaggtcgtg ctgctcggga ccgggaagct
     4381 gctgcgcttc ccgcggcccg agctgatgcg gccgctcgcg caggagcgcg tcggcgtcga
     4441 ggtcatggac ctccaggccg cgtgccgcac ctacaacatc ctcgcctcgg aagggcgccg
     4501 cgtggccgcc gcgctgctct tcgactgaat ggtcgcgggc ccgcgcggct gggtgctgat
     4561 cgcggtcgcg accgcgatct ggttctccgc gctcggcttc cgcacgctgg tcaagcccga
     4621 cgaggggcgt tatgccgaga tcgcgcgcga gatgaacgcg agcggcgacg ggatcacgcc
     4681 gcggctgaac ggcatcaagt atttcgagaa gccgccgctg cagtactggg ccaccgccgc
     4741 cgcgttcacg atcttcggcg agaacgactg gaccgcgcgg ctgtgggccg gcctgaccgg
     4801 cttcctcggc gtgctgattg ccggcgcgct ggcctggcgg ctctacggtc gcctggccgg
     4861 ggtgctgacc gcgacgatgc tagcgacctc ggtcatgtat ttcgtgatcg gccacctgaa
     4921 cacgctcgac atgggcacgg cggtcttcct cgaggccgcg ctcgccgcct tcatcttcgc
     4981 gcaaagtgcc ccgcccgcct cgcgcgcgga gcgccgctgg atgctcgccg cgtgggtcgc
     5041 gatggcgctc gcgactcttt ccaaggggct gatcggcctc gtcttcccgg tgctcacgct
     5101 catcgcctac tcgatcgtcg agcgcgacct gcggccgtgg aagcg
//
LOCUS       DATDMW010000308        14986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1960423, whole genome shotgun sequence.
ACCESSION   DATDMW010000308 DATDMW010000000
VERSION     DATDMW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960423"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..3796
                     /locus_tag="VI319_13060"
     CDS             77..3796
                     /locus_tag="VI319_13060"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEY6864819.1"
                     /translation="MRIRTWASVCAMCLCSALSVHAQDGAWGFLFPYDGNSAPLDFKK
                     FAWGSSPAEACAQRSASELVRIPDTPFTPTVFVGMLPDGSCGFQWLNLCCGSPGALIN
                     FGDVFAQFLSCDQILRMSADVPRPAWCVMQCPADQKNAQGVCRVAGGKDPGPPCPHCG
                     NPVNPGTGNKFESQLDYAGTGPGALRFERFYNSGLPSVERALGKFWQHSYTRKIMQTS
                     PTQATAYRADGKRFQFTLQSGAWVPDGDVADALTRTLDPVLHYTTGWHYQVAADDSVE
                     SYDGSGNPTSIRSRSGLVQTFVYSDGSDGSASGRGGFFLDAAGNPTTAVLPAKILVRI
                     VDHFGRSLAFGYDAQLRIVKLSDPAGGSYLYGYDAKDNVVSVTYPGGARRVYVYNEAA
                     LNGGVDRPNLLTGIVDENGQRFASFGYDAFGRAIVTTHHAGGTEVERYQLAYPVSGAQ
                     TSVVDPLGTVRGFGFQTVVGAVKNTALSQPERGATTAATAYDANGNVASRVDFNGNVA
                     NYTYDLARNLETSRTEAAGTPQARTIATRWHPAFRLRAAVAEPLRITSYVYNGDGGAR
                     CGVQADRTTLVPGVLCSKTVQPTADATGAAGFGAAANGPARTWTYTYDRNGSVLTVDG
                     PRTDVPDITTYAYYANDDPDPGKRGNVATVANALGHVTSITAYNGAGQPTAIVDPNGL
                     VTALAYDARQRLVSRSVGGETTAYEYDPAGQLVKVTLPDGSFIDYAYDGAHRLAGMSD
                     AQGNRIAYTLDAMGNRVREQVLDPLGNLAQTRSRVYDALSRLAQEIGAQDQTTTYAYD
                     AQGNVASVTDPLAHRTVNAYDALNRLAEVTDPALGVTRYAYDGIDQLTSVTDPRGLAT
                     SYAYDGLSNLNRQISPDAGTTSSTYDAAGNLLAQTDAKGQTTSYVYDALNRITSMTFA
                     DGSRQLYAYDRGPNAIGRLAQITEIDAGGLTTSELGYAYDAHGRVVSETRTLAGRSYV
                     TAYGYDAAGRLVGTTYPSGRESAYGLDPLGRIASVATVPPGGAAQDVALDVAYQPFGG
                     VRGYTLGNGRPVVRGHDQDGRIASYTLGARTFAIGYDAASRVSFVADGATPASANTYG
                     YDALDRLTSAVIPGNQYSYAYDAVGNRASKTVGSSTDTYAYDAASNRIASITAQTGAV
                     RHFVLDPNGSTTDDGVNRYAYDARGRMAQSVGALGTTTYQVNALGQRVRKTGAGDDRI
                     FHYDTKGRLIAESDPDGKTRREYLWLGDIPLAVIQ"
     gene            complement(3843..4805)
                     /locus_tag="VI319_13065"
     CDS             complement(3843..4805)
                     /locus_tag="VI319_13065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864820.1"
                     /translation="MMSPTRVLAALALGLAAITAHAQNYPTKAVRLIIPFPPGGSNDV
                     VGRMIAYQLSERLGKQVVADNQGGAGGIIGTEAAAKAAPDGHTLLLISVAHAFGSSMY
                     KLPYDPIGAFAPITLLGTGPVVLCVNPKLPVRSLQDLIDLAKKEPGTLNYATAGVGSF
                     QHLSSALFKLQSGLDIVHVPFKGGGPAMMDVVAGNTQITIGSLIQMLPQIKNGRLRAL
                     GVGSAKRVPAMPDVPTISEAGVPGYEATNWWGIVAPAGTPKPVIDRLHRELVVILKTA
                     ETKKRFESEGGEAVPMTPDEFGRFIVSETAKWAKVVKEAGIKPE"
     gene            4911..5843
                     /locus_tag="VI319_13070"
     CDS             4911..5843
                     /locus_tag="VI319_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864821.1"
                     /translation="MQALKTAIGNYGITRALKDGGVRGGPPLAQIEVSPITAAMRRMV
                     RTLEFDVCEMAFTTYLCARALGKPILAIPAFVTRNFHHWALFKNAKAGIESPKDLEGR
                     VVGVNRGYTVTTGLWARGMLQDEYGVDLDRITWAPTDDEHVAEYKAPANVDYSFRGRA
                     LPDLLASGEIGAAIGDIRSDSPEIRPLIADARNAGFEYYRKTGVYPINHGIVVRAELL
                     RAHPGLARELFDAFKASKSAYLARLREGRDLSPADQTALDLGRVVGGDPFPFGVRANR
                     PALETMVRFAVEQRVIPKAIAVDDLFSPETLALE"
     gene            5884..6798
                     /locus_tag="VI319_13075"
     CDS             5884..6798
                     /locus_tag="VI319_13075"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864822.1"
                     /translation="MNAKALKKVNVSMMNAIHDLPLLVARAEGYFRDEGLDVNILKTP
                     GTGQRDSDHQALRDNIFARTMESLYEQGKCDQFRMCEWGIMKRAVESNVTSGHRPAKI
                     VALGSAMSTFAIVTDPKQRIYEPEQLKNRPIAVSPYNGSHFTLLKMLEGFLKRDQIQW
                     TNAGTMRERIEALVRGDVAAVSLMEPWISVAEKRGLRVLMESHSTRSEAAGDELDGPT
                     LAAMFRAEARAAEAIDKNPLKHARYLVEEAGGLLEPKELKLSRVLNAPPEPYTRERFD
                     HTYQWTLGWGLVAPGATYENTVDNRAWQ"
     gene            6874..7872
                     /locus_tag="VI319_13080"
     CDS             6874..7872
                     /locus_tag="VI319_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864823.1"
                     /translation="MDQRIADPWSLNEADYPARGTREEQLRFALRYAVLAPSNRNSQP
                     WTFTVRADQISVHADPSRWQRVSDPHGHELHVSIGCALENLLVALDHFGLGHVVTRSP
                     GAPDGSIVAQVAILDRPTPAPFRPPTLFKALVARRTDHGGYRDLAVPLAAQRRFARCR
                     DGEDLSLVLTDDPTIRLAVHELMLEGEALGLSNRAYRDELARSIGAGDFGGNWLLTLA
                     QQLAVTHLGSRMADVRGGAEALHSSPLFGVIGAPSASNAMYMRAGELLERVYLVAALQ
                     GLALQPISQLVEASAVKAQFAKLFRAAGVPLVAFRLGYGRAGGRPTPRRALEEVLL"
     gene            7885..8331
                     /locus_tag="VI319_13085"
     CDS             7885..8331
                     /locus_tag="VI319_13085"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY6864824.1"
                     /translation="MLAVPDPLRREHELMRDELARASREPGPVGDCAKRLMRILKPHI
                     DKEETFALPPLGMLAQAVRGIRSPEDEAAVIMAWRLREDLDNMLAEHRVIGAALEELL
                     AAAKAERRVEYAELAARMLHHMQTEEQVLYPATILVGDYLRGKRRT"
     gene            complement(8304..8582)
                     /locus_tag="VI319_13090"
     CDS             complement(8304..8582)
                     /locus_tag="VI319_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864825.1"
                     /translation="MSSIFSGIEKPRVVWAWHVVGTDCRCRLLWKLDDVGARIWAESH
                     ELDLERIDGTDEVRTVAACGVPPRGRALRPAQGRLFEAPAQVRRFPRR"
     gene            complement(8725..9486)
                     /locus_tag="VI319_13095"
     CDS             complement(8725..9486)
                     /locus_tag="VI319_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6864826.1"
                     /translation="MSEDLGGFELDILVQGFPGKMVCHGGLGWSTIALLRGRARVALV
                     DVGAFPMRKGLIEQLARRGLKPADVTDVLLTHSHYDHSVNWTLFRDARIVISGAELAW
                     SLQAPWGETPVPELYVRELARWPGVHRAADGEEVMPGVVAHAAPGHTPGHLVFVASGR
                     SRDAVFTGDAAKNRAELLSRRADATYDAATSSASIAMIRDLWDRRPGSIVVPGHDLPM
                     TQENGRIRYLGEREAALKVWVGDDLETTKLVKLSL"
     gene            complement(9483..10556)
                     /locus_tag="VI319_13100"
     CDS             complement(9483..10556)
                     /locus_tag="VI319_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY6864827.1"
                     /translation="MAEQVYASAGAARAFAHALLAAHALPEADARRSAECLVLADLRG
                     VQTHGLLRLPGYLDRIRKGLVDPKPALSPARVTPVAAALDGRNGLGFVVGTRAMAEAI
                     AMARESGIGVVAVRRSTHFGMAATYVLQALEAGMVSLVFTNASRAMPPWGGREPLLGT
                     SPLAAGVPGGKLAPFVLDMSPSIAARGKIRIAEKRGEKIPLGWALDGEGRPTTDPTAA
                     LAGVVLPIGGPKGSGLAMMMDIFGGVLSGAAYAGDVGDQYKAFDRPQDVGHFFLAMRP
                     DLFVPMDEVRARMDTLVARIKACPRAEGVDEILMPGEIESRLAVARAKAGIPYGAEID
                     AVQAEAKRAGIAPLAVSNQRLGR"
     gene            complement(10549..11361)
                     /locus_tag="VI319_13105"
     CDS             complement(10549..11361)
                     /locus_tag="VI319_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY6864828.1"
                     /translation="MPQAARDVVRNIVKDKLARDEVVASITVRLVRSIEIAMLAKTAG
                     FDSLYVDVEHSSFSLDTTGQICIAALAAGVAPFVRVPTRRPEHVSRVLDGGALGVIAP
                     HIGTPSEAREVVAAAKFPPFGERSMAGPPPHLQYRAFPPAEANAAMNEATMVVVQFET
                     ADALERADEIAAVEGVDLVLMGTNDLLADMGLTGQYEHPRVREAYAHTIAACRRHGKH
                     VGVGGLATRPQLAAEFVQMGARYVSTGTDLAFLLAAATQKAKEAHAMAGAGG"
     gene            complement(11374..11988)
                     /locus_tag="VI319_13110"
     CDS             complement(11374..11988)
                     /locus_tag="VI319_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6864829.1"
                     /translation="MLSFWTMTTAAQTYIEPDTRVGRPYLEAALGAFEPQSTNHLANQ
                     DGQGFGLVGGGYRPTPGFAWGVEVTGFGQRVDTPAGVSAPSGFRSVDSRSRLSTDGLD
                     VNARWIIPFDRWEPFLGAGIGWYQSLLEVRGTSSFFRDTVAEEKSNDFGAHALAGFDY
                     WIRPRIALGAEVRFLRLNARFESLVQGTEHVGGTFLMLRYRQAF"
     gene            12224..14203
                     /gene="asnB"
                     /locus_tag="VI319_13115"
     CDS             12224..14203
                     /gene="asnB"
                     /locus_tag="VI319_13115"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY6864830.1"
                     /translation="MCGIVGYCRLTGAAPAPAPDVIERMTGALRHRGPDGAGAHHDAA
                     VGLGHARLSIIDLEGGAQPLANEDKSVWITFNGEIFNHVELRRQLLGRGHRFRTHSDT
                     ETIVHAYEDLGTGCVDELNGDFAFALWDSRRRRLVLARDRMGVRPLYYTVRDGVLVFA
                     SEVKALLRFPGVEARLDPRALDQVFTFWFPLAPRTPWAGIHELPPGHRLIAENGRIAL
                     ERWWKLDYPRADGRGPALDRRGEAAIAEELEALLIDATRIRLRADVPVGAYLSGGFDS
                     SATTALARLFPGAGLRTFSVAFESAEFDESEHQRQVVAALRTDHTSVRCTRADIASRF
                     PDVIRHTERPVLRTAPAPLYLLSRHVRSSGFKVVVTGEGADEVLGGYDIFKEAKVRRF
                     WARRPASRWRPLLLSRLYPYLEGVQRQPQAYLQAFFGTGLESVDDPLFSHLPRFRLTA
                     RAKAFFSADLRAELRGYDAMAELRESLPPEFARWHPLSQAQYLETAHLLPGYILSSQG
                     DRVAMANAVEGRFPFLDHRVVELAARIPPRLKLKGLREKHILRRAVGKYLPAAIAERP
                     KQPYRSPESECFTGAGAPEYARALLSPPALARSGLFDPAAVEKLASKCDRGAARIGIG
                     DNMALVGILSTQLLDDQFAAGVPARAPDAAATVFA"
     gene            14221..14523
                     /locus_tag="VI319_13120"
     CDS             14221..14523
                     /locus_tag="VI319_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY6864831.1"
                     /translation="MQQADISASVRKFINDNFLFREGIEAIADDASFLDAGIIDSTGV
                     LELVSYLEDEWGITVADDEMVPENFDSIRGLVAYLQRKRDDARALRPGPVPAFAGT"
     gene            14533..>14986
                     /locus_tag="VI319_13125"
     CDS             14533..>14986
                     /locus_tag="VI319_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6864832.1"
                     /translation="MRVEAFLEESRRRHPDKLALVAGGARLSYADLDAAASRCANALR
                     ERGLARGDRVVVFMNNSAEAVVAIFGTLKAGGVFSVVNPGTKADKLAWLLDKYRATAL
                     VTEGRLLPVAAEAIPRAPSLVLALVAGAHDLPAGLVDWDAALAAAPETP"
BASE COUNT         2275 a         5372 c         5222 g         2117 t
ORIGIN      
        1 cgatgtttac aggattgacc ggaaccatcg gttcccggaa agtctctacc gacggaacag
       61 cggccaggga gggctcatgc ggatccggac ttgggcgtcg gtttgcgcca tgtgcctgtg
      121 cagcgccttg tcggtgcacg cgcaggacgg ggcctgggga ttcctgtttc cgtacgacgg
      181 gaattcggct ccgctcgact tcaagaaatt cgcctggggc agctcgcctg cggaggcgtg
      241 cgcgcagcgc agcgcgagcg agctcgtgcg catacccgac acaccgttca cgccgaccgt
      301 cttcgtcggg atgcttccgg acggcagctg cggattccag tggctcaacc tttgctgcgg
      361 atcacccggc gctctgatca acttcggcga tgtgttcgcg cagttcctaa gctgcgatca
      421 gatcctgcgc atgtcggccg acgtcccgag gccggcatgg tgcgtgatgc agtgcccggc
      481 cgaccagaag aatgcgcagg gcgtgtgccg cgtcgcggga gggaaggatc ccggtcctcc
      541 gtgcccgcac tgcggcaacc cggtcaaccc gggcaccggc aacaagttcg agtcgcagct
      601 cgattacgcc ggcaccggtc cgggagcgct gcgcttcgag cgcttctaca acagcggcct
      661 gccctcggtc gagcgcgcgc tcggcaagtt ctggcaacac tcttacacgc gcaagatcat
      721 gcagacttcg ccgacgcagg cgaccgcgta ccgcgcggac ggcaagcgat tccagttcac
      781 gctccagtct ggcgcctggg tcccggacgg ggacgtcgcc gatgcgctga cccggacgct
      841 cgacccggtc ttgcattaca cgaccgggtg gcattaccag gtcgcggccg acgacagcgt
      901 cgagagctac gacggctcgg gcaacccgac atcgatccgc agccgcagcg gcctggtgca
      961 gaccttcgtg tactcggacg gcagcgacgg ctcggcgagc ggcaggggcg ggttctttct
     1021 cgacgcggcc ggaaatccca cgactgcggt gcttccggcg aagatcctgg tgcgcatcgt
     1081 cgatcatttc gggcgctcgc tcgccttcgg ctacgatgcg cagcttcgga tcgtgaagct
     1141 gagcgatccg gccggcggca gctacctgta cggctacgac gcgaaggaca acgtcgtctc
     1201 ggtgacgtac ccgggaggcg cccggcgcgt gtacgtgtac aacgaggcag cgctgaacgg
     1261 cggcgtcgac cggccgaacc tcctgaccgg catcgtcgac gagaacggac agcggttcgc
     1321 gagcttcggg tacgacgcgt tcggccgggc gatcgtcacc acgcaccacg ccggcgggac
     1381 cgaggtcgag cgctaccagc tggcgtatcc cgtgtcgggt gcgcagacga gcgtcgtcga
     1441 tccgctcggc accgtgcgcg gcttcggctt tcagacggtg gtcggcgcgg tgaagaacac
     1501 ggcgctcagc cagcccgagc gcggcgcgac caccgccgcg acggcgtacg acgcgaacgg
     1561 caatgtcgcg agccgcgtcg acttcaacgg caacgtcgcc aattacacct acgaccttgc
     1621 gcgcaatctc gagacctcgc gcaccgaagc cgcgggcacg ccgcaggcgc gcaccatcgc
     1681 cacacgctgg cacccggcct tccgcctgcg cgcggcggtg gccgagccgc tgcggatcac
     1741 gagctacgtc tacaacggcg acggcggtgc gcgctgcggc gtgcaggccg accgcacgac
     1801 gctcgttccg ggcgtgttgt gctcgaagac ggtgcagccg accgccgacg cgaccggcgc
     1861 cgcgggcttc ggcgcggcgg caaacggacc ggcgcgcacc tggacctaca cctacgaccg
     1921 caacggctcg gtgctgacgg tcgacggccc gaggaccgac gtccccgaca tcacgacgta
     1981 cgcgtactac gcgaacgacg atccggaccc gggcaagcgc ggcaacgtcg cgacggtcgc
     2041 gaacgcgctc ggtcacgtca cgtcgatcac cgcctacaac ggcgccggcc agccgaccgc
     2101 gatcgtcgat cccaacggcc tcgtcaccgc gctcgcgtac gacgcgcgcc agcggctcgt
     2161 gtcgcgctcg gtaggcggcg agacgaccgc gtacgagtac gatcccgcgg ggcagctcgt
     2221 gaaggtcacc ctgcccgacg gctcgttcat cgactacgca tacgacggtg cgcaccggct
     2281 cgccggcatg tcggacgcgc agggcaaccg gatcgcctac acgctcgatg cgatgggcaa
     2341 ccgcgtgcgc gagcaggtgc tcgatccgct gggaaatctc gcgcagacgc ggagccgcgt
     2401 gtacgacgcg ctcagccgcc tcgcgcagga gatcggggcg caggaccaga cgaccaccta
     2461 cgcgtacgac gcgcagggga acgtcgcctc ggtgaccgat ccgctcgcgc accgcacggt
     2521 caacgcgtac gacgccctga accggctggc cgaggtcacc gaccccgcgc tcggcgtcac
     2581 gcgctatgcc tacgacggca tcgaccagct gacgagcgtc accgatccga ggggcctcgc
     2641 gacgagctac gcctacgacg gtctgtcgaa cctgaaccgg cagatctcgc ccgacgccgg
     2701 gacgacgagc agcacgtacg acgcggcggg caacctcctc gcgcagaccg acgccaaggg
     2761 gcagaccacg agctacgtgt acgatgcgct gaaccgcatc acgtcgatga ccttcgccga
     2821 cggctcgcgc cagctgtatg cctacgaccg agggccgaac gcgatcgggc gcctcgcgca
     2881 gatcacggaa atcgacgcgg gcgggctgac gacgagcgag ctcgggtacg cgtacgacgc
     2941 gcacgggcgc gtcgtgtccg agacgcgcac gctcgcgggc cgaagctacg tgaccgcgta
     3001 cggctacgac gcggccgggc gcctcgtcgg cacgacgtac ccgagcgggc gcgagagcgc
     3061 gtacggtctc gatccgctcg ggcgcatcgc gtcggtcgcg acggtgcctc ccggcggcgc
     3121 ggcgcaggac gtcgcgctcg acgtcgccta ccagcccttc ggcggcgtgc gcggctacac
     3181 gctcgggaac ggacggccgg tcgtgcgcgg ccacgaccag gacgggcgca tcgcgagcta
     3241 cacgctgggc gcgcgcacct tcgcgatcgg ctacgacgcg gcgagccgcg tgagcttcgt
     3301 cgccgatggc gccactcccg cgagcgcgaa cacgtatggc tacgacgcgc tcgaccgcct
     3361 cacgagcgcg gtgatcccgg ggaatcagta cagctacgcg tacgacgcgg tggggaaccg
     3421 cgcgtcgaag acggtgggga gcagcacgga cacttacgct tacgacgccg cgagcaaccg
     3481 catcgcgagc atcaccgcgc aaaccggggc cgtgcgtcat ttcgtgctcg acccgaacgg
     3541 ctcgacaacc gacgacggcg tcaaccgcta cgcctacgac gcgcgcgggc gcatggcgca
     3601 gtccgtcggc gcgctcggga ccacgaccta ccaggtgaac gcgctcggcc agcgcgtgcg
     3661 caagaccggc gcgggcgacg accgcatctt ccactacgac acgaaagggc ggctgatcgc
     3721 ggagagcgat ccggacggga agacccggcg agaatatctc tggctgggcg acattccgct
     3781 ggcggtgatc cagtgacccc gctcgaggag gtcacaattt gcgacgaccg cgcttacaaa
     3841 gcttactccg gcttgatccc cgcctccttc accaccttcg cccacttcgc cgtctccgac
     3901 acgatgaacc gcccgaactc gtcgggcgtc atcggcaccg cctcgccgcc ctcggactcg
     3961 aagcgcttct tcgtctcggc ggtcttgagg atgacgacga gctcccggtg caggcggtcg
     4021 atcacgggct tgggcgtgcc cgcgggcgcg acgatgcccc accagttcgt cgcctcgtag
     4081 ccggggacac cggcttccga gatcgtcggg acgtcgggca tcgcgggcac gcgcttcgcg
     4141 ctgccgacgc cgagcgccct cagccgtccg ttcttgatct ggggcagcat ctggatgagc
     4201 gagccgatcg tgatctgggt gttgcccgcg acgacgtcca tcatcgcggg accgccgccc
     4261 ttgaacggaa cgtgcacgat gtcgaggccc gactgcagct tgaacagcgc gctcgacagg
     4321 tgctggaagc tgccgacgcc ggcggtcgcg tagttgagcg ttccgggctc cttcttcgcg
     4381 aggtcgatga ggtcctgcag gctgcgcacc ggcagcttgg gattcacgca cagcacgacg
     4441 ggcccggtcc cgagcagggt aatcggcgca aaggcgccga tcgggtcgta aggcagcttg
     4501 tacatcgagc tgccgaaggc gtgcgcgacc gagatcagga gcagcgtgtg gccgtcgggg
     4561 gcggccttgg ccgcggcctc ggtgccgatg atgccgcccg cgccgccctg gttgtcggcg
     4621 acgacctgct tcccgaggcg ctcggacagc tggtacgcga tcatgcggcc gacgacgtcg
     4681 ttcgagccgc cgggcgggaa cgggatgatc agccgcaccg ccttcgtcgg gtaattctgg
     4741 gcgtgcgctg ttatcgcggc gaggccgagc gccagcgccg ccaggactct ggtcggtgac
     4801 atcatggttg cctcctccgg cgaatattac tatcagtaac ggggattgcc ccggtggcgc
     4861 ggacaactta tcctgggacc tgaagtcgaa gaatccggag gccctccctg atgcaagccc
     4921 tcaagaccgc catcggcaat tacggcatca cgcgcgcgct caaggacggc ggcgtccgcg
     4981 gcggaccgcc gctggcgcag atcgaggtga gcccgattac cgcggcgatg cggcgcatgg
     5041 tgcgcacgct cgagttcgac gtgtgcgaga tggcgttcac gacctacctg tgcgccaggg
     5101 cgctgggcaa gccgatcctc gcgatcccgg ccttcgtgac gcgcaacttc catcactggg
     5161 cgctgttcaa gaacgcgaag gccggcatcg agtcgccgaa ggacctcgag ggccgggtgg
     5221 tcggcgtgaa ccgcggctac acggtgacga ccggactgtg ggcgcgcggg atgctgcagg
     5281 acgagtacgg cgtggacctg gaccgcatca cctgggcgcc gaccgacgac gagcacgtcg
     5341 ccgagtacaa ggcgcccgcg aacgtcgact actcgttccg cggccgggcg cttcccgacc
     5401 tcctcgcctc gggcgagatc ggcgcggcga tcggcgacat ccgcagcgac tcgccggaga
     5461 tccggccgct gatcgccgac gcccgcaacg ccggcttcga gtactaccgc aagaccggcg
     5521 tgtacccgat caaccacggc atcgtcgtgc gcgccgagct gctgcgcgcc catcccgggc
     5581 tcgcgcgcga gctgttcgac gccttcaagg cgtccaagtc ggcttacctc gcgcgcctgc
     5641 gcgaagggcg cgacctgtcg cccgccgatc agaccgcgct cgacctcgga cgcgtggtcg
     5701 gcggcgaccc gtttccgttc ggcgtgcgcg cgaaccggcc cgcgctcgag accatggtgc
     5761 gcttcgcggt cgagcagcgc gtgatcccga aggcgatcgc ggtcgacgac ctgttctcgc
     5821 cggagacgct cgcgctcgaa tagatccgtc ttctccattc acccacccga gaaagggcaa
     5881 gcgatgaacg caaaggctct gaagaaggtg aacgtgtcga tgatgaacgc gatccacgac
     5941 ctgccgctgc tggtcgcgcg cgccgaggga tacttccgcg acgagggcct cgacgtcaac
     6001 attctgaaga cgccggggac gggccagcgc gactccgacc accaggcgct gcgcgacaac
     6061 atcttcgcgc gcaccatgga gtcgctctac gagcagggca agtgcgacca gttccgcatg
     6121 tgcgagtggg gcatcatgaa gcgtgcggtc gagtcgaacg tgacctcggg ccaccgcccg
     6181 gcgaagatcg tggcgctcgg ctccgcgatg tcgaccttcg ccatcgtcac cgacccgaag
     6241 cagcgcatct acgagcccga gcagctcaag aaccggccga tcgcggtcag cccctacaac
     6301 ggctcgcact tcaccctgct caagatgctc gagggcttcc tcaagcgcga ccagatccag
     6361 tggacgaacg ccggcaccat gcgcgagcgc atcgaggcgc tcgtgcgcgg cgatgtcgcc
     6421 gcggtgagcc tgatggagcc gtggatctcg gtcgcggaga agcgcggcct gcgcgtgctc
     6481 atggagtcgc actcgacgcg cagcgaggcc gcgggcgacg agctcgacgg gccgacgctc
     6541 gccgcgatgt tccgcgccga ggcgcgcgcc gccgaggcga tcgacaagaa cccgctcaag
     6601 catgcgcgct acctggtcga ggaggcgggc ggcctgctcg agcccaagga gctcaagctc
     6661 tcgcgcgtgc tcaacgcgcc gcccgagccg tacacgcgcg agcgcttcga ccacacctac
     6721 cagtggaccc tcggctgggg gctcgtcgcc ccgggcgcca cgtacgagaa caccgtcgac
     6781 aaccgcgcgt ggcagtgagg cgcttgatct gaatcaaata cggcggccgg cgttcggtca
     6841 ccatgggatt caatggatcc catggcttga ccgatggacc agcgcatcgc cgatccctgg
     6901 tcgctgaacg aggccgatta cccggcgcgc ggcacgcgcg aggagcagct gcgcttcgcc
     6961 ctgcgctacg ccgtccttgc gccgtcgaat cggaactccc agccgtggac cttcacggtg
     7021 cgggccgacc agatcagcgt ccacgccgac ccgtcgcgct ggcagcgcgt gtcggacccg
     7081 cacggtcacg agctgcacgt cagcatcggc tgcgcgctcg agaacctgct cgtcgcgctg
     7141 gatcatttcg gcctgggcca cgtcgtgacg cggtcgcccg gggcacccga cggcagcatc
     7201 gtggcgcagg tcgcgatact cgaccggccg acgccggcgc cgttccgccc gccgacgctt
     7261 ttcaaggcgc tggtcgcgcg ccgcaccgac cacggcggct atcgcgatct cgccgtgccc
     7321 ctggcggcgc agcggcggtt cgcgcgctgc cgcgacggcg aagatctgtc cctcgtgctc
     7381 acggacgacc cgacgattcg cctggccgtg cacgagctca tgctcgaggg cgaggcgctc
     7441 ggtctatcga accgcgccta tcgcgacgag ctcgcgcgat cgatcggcgc gggcgacttc
     7501 ggcggcaact ggctgctgac actcgcgcag cagctcgcgg tcacgcatct cgggtcccga
     7561 atggcggacg tccgcggcgg ggcggaggcg ctgcacagct cgcccttgtt cggcgtgatc
     7621 ggcgccccga gcgccagcaa cgcgatgtac atgcgggccg gcgagctgct cgagcgcgtg
     7681 tacctggtcg cggcgctgca gggcttggcg ctgcagccga tcagccagct ggtcgaagcg
     7741 tcggccgtca aggcgcagtt cgcgaagctg ttccgcgccg ccggcgtgcc gctggtcgcc
     7801 ttcaggctcg gctacggccg ggccggcggc cggcccacgc cccggcgcgc gctcgaggag
     7861 gtgctgttgt gaaggcgaat cgccatgctc gccgtccctg atccgctccg ccgcgagcac
     7921 gagctgatgc gcgacgagct cgcccgcgcc tcgcgcgagc cgggaccggt cggcgattgc
     7981 gccaagcggc tgatgcggat tctcaagccg cacatcgaca aggaagagac gttcgcgctg
     8041 ccgccgctcg ggatgctggc gcaggcagtg cgcgggatcc ggtcgcccga ggacgaggcc
     8101 gcggtgatca tggcgtggcg gctgcgggag gacctcgaca acatgctggc cgagcaccgc
     8161 gtcatcggcg cggccctcga ggagctgctc gcggccgcca aggccgagcg gcgcgtggag
     8221 tatgccgagc tcgcggcccg gatgctccac cacatgcaga ccgaggagca ggtgctctac
     8281 cccgcgacga tcctcgtcgg cgactatctg cgcgggaagc gccgcacctg agcaggtgcc
     8341 tcgaagagcc tgccctgagc cggtcgaagg gcgcgcccgc gcggcggcac gccgcacgcc
     8401 gcgacggtcc tgacctcgtc ggtgccgtcg atgcgctcga ggtcgagctc gtggctctcg
     8461 gcccagatac gcgcgccgac gtcgtcgagc ttccacagca gccggcagcg gcagtcggta
     8521 ccaacgacgt gccaggccca gacgacccga ggcttctcga tccctgagaa aatgctgctc
     8581 ataccgctgc gacaggccgg aaggcgcccg gtttcatgcc gtttcgtctt gcaccgtagc
     8641 ttgttcggcg gagggccgat ttgcgcgtcc ttcgagcggc tcaggacatg cttcgacaag
     8701 ctcagcgcga acgggccgcg cggctcacaa cgacagcttc acgagcttcg tcgtttccag
     8761 atcgtcgccg acccacacct tgagcgccgc ttcgcgctcg ccgaggtagc ggatgcggcc
     8821 gttctcctgg gtcatcggca ggtcgtggcc cggaacgacg atgctgcccg gacgccggtc
     8881 ccacaggtcc cggatcatgg cgatgctcgc gctgctcgtc gccgcgtcgt aggtcgcatc
     8941 ggcgcggcgc gacagcagct cggcgcggtt cttggcggcg tcgccggtga acacggcgtc
     9001 gcgcgagcgc ccggaggcga cgaacaccag gtgccccggc gtgtggccgg gcgccgcgtg
     9061 cgcgacgact cccggcatga cctcctcgcc gtcggcggcg cggtgcacgc cgggccagcg
     9121 cgcgagctcg cgcacgtaga gctcgggcac cggcgtctcg ccccacggcg cctggagcga
     9181 ccacgcgagc tccgcgcccg agatcacgat gcgcgcgtcg cggaacaacg tccagttgac
     9241 cgagtggtcg tagtgcgaat gcgtgagcag gacgtcggtc acgtcggcgg gcttcaggcc
     9301 gcggcgcgcg agctgctcga tcagcccctt gcgcatcggg aacgcgccga cgtcgaccag
     9361 cgccacgcga gcgcgcccgc gcagcagcgc gatcgtgctc cagccgaggc cgccgtggca
     9421 caccatcttg cccgggaagc cctggaccag gatgtcgagc tcgaagccgc cgaggtcttc
     9481 gctcatcggc cgagacgctg attcgacacc gcgagcgggg cgatgcccgc gcgcttcgcc
     9541 tcggcctgca cggcgtcgat ctcggcgccg tagggaatgc cggccttcgc gcgcgccacg
     9601 gcgaggcgcg actcgatctc acccggcatc aggatctcgt cgacaccctc ggccctggga
     9661 caggccttga tgcgcgcgac cagcgtatcc atgcgcgcgc gcacctcgtc catcggcacg
     9721 aacaggtcgg gccgcatggc gaggaagaaa tgaccgacgt cctgcggccg gtcgaacgcc
     9781 ttgtactggt cgccgacgtc gcccgcgtac gccgcgcccg agagcacgcc gccgaagatg
     9841 tccatcatca tcgcgaggcc cgagcccttc gggccgccga tcggaagcac gacgccggcg
     9901 agcgccgcgg tcggatcggt cgtcggccgg ccttcgccgt cgagcgccca gccgagcggg
     9961 atcttctcgc cgcgcttctc ggcgatgcgg atcttgccgc gcgcggcgat cgagggcgac
    10021 atgtcgagca cgaagggcgc gagcttgccg ccgggcacgc cggcggccag gggactggtc
    10081 ccgagcagcg gctcgcgtcc gccccacggc ggcatcgcgc gcgacgcgtt ggtgaagacg
    10141 agcgacacca tcccggcctc gagcgcctgc agcacgtagg tcgcggccat gccgaagtgc
    10201 gtgctgcggc ggaccgccac gacgccgatg cccgactcgc gcgccatcgc gatcgcctcg
    10261 gccatcgcgc gcgtgccgac gacgaagccg aggccgttcc ggccgtcgag cgccgcggcg
    10321 accggcgtca cgcgcgcggg cgagagcgcg ggcttcgggt ccaccagccc ctttcgtata
    10381 cggtcgaggt aaccgggcag gcgcagcagg ccgtgcgtct gcacgccgcg caggtcggcg
    10441 aggaccaggc actcggccga gcggcgcgcg tccgcctcgg gcagcgcgtg ggcggcgagg
    10501 agcgcgtgcg cgaaggcgcg cgcggccccg gccgacgcgt agacctgctc agccaccggc
    10561 cccggccatc gcgtgcgcct ctttcgcctt ctgcgtcgcc gccgcgagca ggaacgcgag
    10621 gtcggtgccg gtcgagacgt agcgcgcgcc catctgcacg aactcggcgg cgagctgcgg
    10681 ccgcgtcgcg aggccgccca cgccgacgtg cttgccgtgg cggcggcacg ccgcgatcgt
    10741 gtgcgcgtac gcctcgcgca cgcgcgggtg ttcgtactgc ccggtgagcc ccatgtcggc
    10801 gagcaggtcg ttcgtgccca tcagcaccag gtcgacgcct tcgaccgcgg cgatctcgtc
    10861 ggcgcgctcg agcgcgtcgg cggtctcgaa ctgcaccacc accatggtcg cctcgttcat
    10921 cgcggcgttc gcttccgcgg gcgggaacgc gcggtactgg aggtgcggcg gcgggcccgc
    10981 catcgagcgc tcgccgaagg gcggaaactt ggcggcggcg acgacctcgc gcgcctccga
    11041 cggcgtgccg atatgcggcg cgatcacgcc gagcgcgccg ccgtcgagga cgcgcgaaac
    11101 gtgctcgggg cgccgggtcg gcacgcgcac gaagggcgcg acgccggcgg cgagcgccgc
    11161 gatgcagatc tggccggtcg tgtcgagcga gaagctcgag tgctcgacgt cgacgtacag
    11221 gctgtcgaag cccgcggtct tcgcgagcat cgcgatctcg atcgagcgca ccagccgcac
    11281 ggtgatcgac gcgaccacct cgtcgcgggc gagcttgtcc ttgacgatgt tgcgcaccac
    11341 gtcgcgggcc gcctgcggca tgcgctctcc gggtcagaac gcctggcggt agcgcagcat
    11401 gaggaaggtg ccgccgacgt gctcggtccc ctgcaccagc gactcgaagc gcgcgttcag
    11461 ccgcaggaag cgcacctcgg cgccgagcgc gatgcgcggc cggatccagt agtcgaaccc
    11521 ggcgagcgcg tgcgcgccga aatcgttcga cttctcctcg gcgaccgtgt cgcggaagaa
    11581 ggagctcgtg ccgcgcacct cgagtagcga ctggtaccag ccgatgccgg cgccgaggaa
    11641 cggctcccag cggtcgaacg ggatgatcca gcgcgcgttc acgtcgagcc catcggtcga
    11701 gaggcgcgac cggctgtcga cgctgcggaa gcccgaaggc gcggagacgc ccgccggcgt
    11761 gtcgacccgc tggccgaagc cggtcacctc gacgccccag gcgaatcccg gcgtcggccg
    11821 gtagccgccg ccgacgaggc cgaagccctg gccgtcctgg ttggcgagat ggttggtgga
    11881 ctgcggctcg aaggcgccga gcgccgcttc gagatacggc cggccgacgc gcgtgtcggg
    11941 ctcgatgtag gtctgcgcgg cggtcgtcat agtccagaag gagagcacgg ctgctgctac
    12001 ggccgtgcga cgcgtgcggt agaaatggtc catggacgaa acgatccact gggtgaagcg
    12061 agctccgagc atacaccggg cggcccggcc tgtcgcccgg ggccgactcc gatcggccta
    12121 tgcgttcatg acaccacgac gctcggtttc aatgacgccg ctataccctt tcgacgggac
    12181 gacgcatggc gaataaggac tataccgggc tgagggcgcg cggatgtgcg gcatcgtagg
    12241 ctactgccgg ctcacgggcg ctgcgcccgc gccggcgccc gacgtgatcg agcgcatgac
    12301 gggcgcgctg cgccaccgcg ggcccgacgg cgccggcgcg caccacgacg ccgcggtcgg
    12361 gctcgggcac gcgcgcctct cgatcatcga cctcgagggc ggcgcgcagc cgctcgcgaa
    12421 cgaggacaag agcgtgtgga tcacgttcaa cggcgagatc ttcaaccacg tcgagctgcg
    12481 ccgccagctg ctcggccgcg gccaccgctt ccgcacgcat tccgataccg agaccatcgt
    12541 gcacgcgtac gaggacctcg gcacgggctg cgtcgacgag ctgaacggcg acttcgcgtt
    12601 tgcgctctgg gactcgcggc gccggcggct cgtgctcgcg cgcgaccgca tgggcgtgcg
    12661 gccgctctac tacacggtgc gcgacggcgt gctcgtcttc gcctccgagg tgaaggcgct
    12721 gctgcgcttc ccgggggtcg aggctcgcct cgatccgagg gccctcgatc aggtcttcac
    12781 cttctggttc ccgctcgccc cgcgcacgcc gtgggccggc attcacgagc tgccgccggg
    12841 tcatcgactc atcgccgaga acgggcgcat cgcgctcgag cgctggtgga agctcgacta
    12901 ccctcgcgcc gacggccgcg gtcccgcgct cgaccgccgc ggcgaggcgg cgatcgcgga
    12961 ggagctggaa gcgctgctca tcgacgcgac ccgaatccgg ctgcgcgccg acgtccccgt
    13021 cggcgcctat ctcagcggcg gcttcgattc ctcggccacg accgcgctcg cgcggctgtt
    13081 cccgggggcg ggcctgcgca ccttctcggt cgccttcgag tcggccgagt tcgacgagag
    13141 cgagcaccag cggcaggtgg tcgcggcgct gcgcaccgac cacacgagcg tgcgctgcac
    13201 gcgcgccgac atcgcatcgc gcttccccga cgtgatccgg cacaccgagc ggcccgtgct
    13261 gcgcaccgcg cccgcgccgc tgtacctgct ctcgcgccac gtgcgctcga gcggcttcaa
    13321 ggtggtcgtg accggcgagg gcgccgacga ggtgctcggc ggctacgaca tcttcaagga
    13381 ggcgaaggtg cgccgcttct gggcgcggcg gcccgcctcg cgctggcggc cgctgctgct
    13441 gtcgcgcctg tatccctacc tcgagggcgt gcagcgccag ccgcaggcct acctgcaggc
    13501 cttcttcggc acggggctcg agtccgtcga cgacccgctg ttctcgcacc tgccgcgctt
    13561 ccgcctcacc gcgcgcgcga aggcgttctt ctccgcggac ctgcgcgccg agctgcgcgg
    13621 ctacgacgcg atggccgagc tgcgcgagtc gctgccgccg gagttcgccc gctggcatcc
    13681 gctgtcgcag gcgcagtacc tcgagaccgc gcacctgctc cccgggtaca tcctgtcctc
    13741 gcagggcgac cgcgtcgcga tggcgaacgc ggtcgaaggc cgcttcccgt tcctcgacca
    13801 tcgcgtggtc gagctcgccg cgcgcatccc gccgcgcctg aagctgaagg gcctgcgcga
    13861 gaagcacatc ctccggcgcg ccgtcggcaa gtacctgccc gccgcgatcg cggagcgccc
    13921 gaagcagccg taccgctcgc ccgagagcga gtgcttcacg ggggccggcg ccccggaata
    13981 cgcccgtgcg ctgctttcgc cgccggcgct cgcgcgctcc gggctcttcg acccggccgc
    14041 ggtggagaag ctcgcgtcga agtgcgaccg cggcgccgcc cggatcggca tcggcgacaa
    14101 catggcgctc gtcggcatcc tgtcgacgca gctgctcgac gaccaattcg ccgccggcgt
    14161 cccggcccgt gcgcccgacg ccgcggcaac cgtttttgcg tagacgaaca aggaagctcc
    14221 atgcagcagg ccgacatcag cgcgtcagtc cgaaaattca tcaacgacaa cttcctgttc
    14281 cgggaaggca tcgaggcgat cgccgacgat gcgtccttcc tggatgcggg aatcatcgac
    14341 tcgaccggcg tcctcgagct cgtctcctac ctcgaggacg agtggggcat cacggtcgcc
    14401 gacgacgaga tggtgcccga gaacttcgac tcgatccgcg gcctggtcgc ctacctgcag
    14461 cgcaagcgcg acgacgcgcg cgcgctgcgc cccggccccg tgcccgcgtt cgccgggacc
    14521 tgaggagccg acgtgcgagt cgaggccttc ctggaagaga gccggcggcg ccatccggac
    14581 aagctcgcgc tggtcgcggg cggcgcgcgc ctctcgtacg ccgatctgga cgccgcggcg
    14641 agccgctgcg cgaacgcgct gcgcgagcgc ggtctcgcgc gcggcgaccg cgtggtcgtc
    14701 ttcatgaaca actccgccga ggcggtcgtc gcgatcttcg gcaccctgaa ggcgggcggc
    14761 gtgttcagcg tggtgaaccc gggcaccaag gccgacaagc tcgcctggct gctcgacaag
    14821 taccgcgcga cggcgctggt cacggaaggc cggctgctcc cggtcgcggc cgaggccatc
    14881 ccgcgcgcgc cctcgctcgt gctcgcgctg gtcgcgggcg cgcacgacct gcccgccggt
    14941 ctggtcgact gggacgcggc gctcgccgcg gcgcccgaga cgccgg
//
LOCUS       DATDMW010000309         2190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_467127, whole genome shotgun sequence.
ACCESSION   DATDMW010000309 DATDMW010000000
VERSION     DATDMW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467127"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..>2190
                     /gene="glnE"
                     /locus_tag="VI319_13130"
     CDS             171..>2190
                     /gene="glnE"
                     /locus_tag="VI319_13130"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEY6864833.1"
                     /translation="MDLLEYSRYARHVLGARPELRAEIGPAEGWTRDAMRAFLAGRPI
                     ADEASLHAALRSLRQRVVLRTMSRDLAGQAPLAEVCAAMSSLADVAVESALAFLEPEL
                     ESTLGIPVAGGERQRLVVIGMGKLGGGELNVSSDVDLVFLYPEEGETSPSTSSGRSVS
                     NLEFFTRLGRRLIAALGEATGDGFVFRVDMRLRPWGDDGPLATPFDALEEYFVTQGRE
                     WERYAWIKARALAGRDHPALAGIVRPFVFRKYLDYGAIAAMRELHAQIRAEVARRELA
                     DHIKLGPGGIREIEFIAQAFQLIRGGRDASLQARPTLEVLQLLAAKTLLPRAAVDELS
                     EAYVFLRRLEHRLQYLDDAQTHELPGNDEDRARVARAMGFDSWDAFREDLERHRARVS
                     WHFEQVFSVEERPRHALAPIWTAPPDDAAAKLAALGFGDAGSAAARLAAVRSGSRYGG
                     LPQASRERFDELVPRLLEAAAAQENPDTALARGLDFAETISRRAAYLALLDEHPAVLA
                     RLALLLGASSWAAEYLNRHPVLVDELLDARTLFAAPDWSAFAQGLRRELAARAGDEER
                     QMDWLREAHHAQVFRLLAQDLAGELTVERLADHLSDLADVMLQVTLELAWSQLRVRHR
                     DIPRFAVIAYGKLGGKELGYGSDLDVVFLYDDTDEPDHDRAAEVYAAFV"
BASE COUNT          279 a          811 c          787 g          313 t
ORIGIN      
        1 ttcgacgtgc acgaacggtc ccggaagcct gtgtcgcctc acggcaacgg agttgggggt
       61 gccggcatac tacaatccgc ggctattcgg attcctgaac gcgggaccgg gcgcgcacgc
      121 cgacacatgt cacgggcgag cccgcgcgcc gcgtcatttt acccgcaccc gtggacctgc
      181 tcgaatattc ccgctacgcg aggcacgtgc tcggcgcgcg gcccgagctg cgcgccgaga
      241 tcggcccggc cgaggggtgg acgcgcgacg cgatgcgcgc cttcctcgcc gggcgcccca
      301 tcgccgacga ggcctcgctg cacgcggcgc tgcgctcgct gcgccagcgc gtcgtcctgc
      361 gcaccatgtc ccgcgacctc gcggggcagg cgccgctcgc cgaggtctgc gccgcgatgt
      421 cgtcgctcgc cgacgtcgcg gtcgagagcg cgctcgcctt cctcgagccc gagctcgaga
      481 gcacgctcgg catcccggtc gcgggcggcg agcgccagcg gctggtcgtg atcggcatgg
      541 gcaagctcgg gggcggcgag ctgaacgtgt cctcggacgt cgatctcgtg tttctctatc
      601 ccgaagaggg agagacaagc ccgtcgacga gctcggggcg atcggtttcg aacctcgagt
      661 tcttcacgcg cctcgggcgg cgcctgatcg ccgcgctcgg cgaggcgact ggcgacggct
      721 tcgtgttccg cgtcgacatg cgcctgcgtc cctggggcga cgacgggccg ctcgcgactc
      781 ccttcgacgc gctcgaggag tacttcgtga cccaggggcg cgagtgggag cgctacgcct
      841 ggatcaaggc gcgcgcgctc gccggccgcg atcatcccgc gctcgcgggc atcgtgcggc
      901 ccttcgtctt ccgcaagtac ctcgactacg gcgcgatcgc ggccatgcgc gagctgcacg
      961 cgcagatccg cgccgaggtc gcgcggcgcg agctcgccga ccacatcaag ctcgggcccg
     1021 ggggcatccg cgagatcgag ttcatcgcgc aggcgttcca gctgatccgc ggcggccgcg
     1081 acgcctcgct gcaggcacgc cccaccctcg aggtgctgca gctcctcgcg gcgaagaccc
     1141 tgctgccgcg cgcggctgtg gacgagctct ccgaagcgta cgtgttcctg cgccgcctcg
     1201 agcaccgcct gcaatacctc gacgacgcgc agacgcacga gctgcccggg aacgacgagg
     1261 accgcgcgcg cgtggcgcgc gcgatgggct tcgattcctg ggacgccttc cgcgaggacc
     1321 tggagcggca tcgggcgcgc gtgtcgtggc atttcgagca ggtgttctcg gtcgaggagc
     1381 ggccgcggca cgcgctcgcg ccgatctgga ccgcgccgcc ggacgacgcg gccgcgaagc
     1441 tcgccgcgct cggcttcggc gatgccggct cggccgccgc gcgcctcgcc gccgtgcgct
     1501 cgggcagccg ctacggcggc ctgccgcagg cgagccgcga gcgcttcgac gagctggtac
     1561 cgcggctgct cgaggcggcg gcggcgcagg agaatccgga cacggctctc gcgcgcggcc
     1621 tcgacttcgc cgagacgatc agccgccgcg cggcctacct cgcgctcctc gacgagcatc
     1681 ccgcggtgct cgcgcggctc gcgctgctgc tcggcgcgtc gagctgggcg gcggagtacc
     1741 tgaaccgcca cccggtgctg gtcgacgagc tgctcgatgc gcgcacgctg ttcgccgcgc
     1801 ccgactggag cgcgttcgcg cagggactgc ggcgcgagct cgccgcgcgc gccggcgacg
     1861 aggagcggca gatggactgg ctgcgcgagg cgcaccacgc gcaggtgttc cggctgctcg
     1921 cgcaggacct cgcgggcgag ctgacggtcg agcggctcgc cgatcacctg tcggacctcg
     1981 ccgacgtcat gctgcaggtg acgctcgagc tcgcctggtc gcagctgcgc gtgcgccacc
     2041 gcgacatccc gcgctttgcc gtgatcgcct acggcaagct cggcggcaag gagctcggct
     2101 acggcagcga tctcgacgtg gtcttcctct acgacgacac cgacgagccc gatcacgacc
     2161 gcgcagcgga ggtctacgcc gccttcgtgc
//
LOCUS       DATDMW010000310         2754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1587557, whole genome shotgun sequence.
ACCESSION   DATDMW010000310 DATDMW010000000
VERSION     DATDMW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587557"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VI319_13135"
     CDS             <1..1018
                     /locus_tag="VI319_13135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY6864834.1"
                     /translation="IGDLVCTTPLVAALRRALPDAFIAALVNSYNREVLDGNPDVDRV
                     YAYGKAKHRSGRESLLGTYLRRAELVLELRRERFDYAVLAAPGFQPRSLRLARLAGAR
                     HVVGFTEGRARAERRVDCAVPYGAPAARHEVEDVLRLLGPLGISAPEPRLRVVPDPGL
                     AETMRANVAQRMPGGGPLVALHISARKPSQRWPAERFAALARALYERASAQHERPRFV
                     LLWAPGSERNPTHPGDDEKARALVAMLEGLPALPVPTFRLAELIAALDACDAVVCSDG
                     GAMHLAAALGKPIVCFFGDSQPSRWRPWGVRHELLQPASRDAVDIGVEQALAAFERLH
                     ASAR"
     gene            complement(1015..>2754)
                     /locus_tag="VI319_13140"
     CDS             complement(1015..>2754)
                     /locus_tag="VI319_13140"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEY6864835.1"
                     /translation="NFEIPEPFQESLLRSLEWAVPVQMVVFLLSGLYRGMWRFASLTD
                     LKHIALAVGLSAALVATVLYMAQPELLVPRSVLILDPVLLVLAMSGSRLAYRYLREHR
                     LRDVLAGTRKAVLVLGAGEAAEKLIAELARSQEWRIVGLLDDDPVKQGRDIHGIRVLG
                     PLDALPRYARREGAEHAIIAMPGVAHAIRRRAADLCAAAGLRVMTVPSFDDLVGGKVT
                     ISQIRNIELDDLLGRDPVRLDSAGLSGWLEGSTVLVTGAGGSIGAELSRQVARFRPRL
                     LVLLELNEYALYTIEQEFAARMPEVAIECVIGDVRSAARVAEILGRVRPEVVFHAAAY
                     KHVPLMERDNAWEAVQNNVRGTLTIARAAQASGVEKFVLVSTDKAVNPANVMGATKRL
                     AEMVCGALQRGSSTSFVTVRFGNVIGSTGSVIPKFREQIAAGGPLTVTHPEVTRYFMS
                     IPEAAQLVLQAGWMGRGGEIYVLEMGTPIRIVDLAREMIRLSGFSETDVRIEFVGLRP
                     GEKLYEEPISDQEQTLPTPHPMLRVARSSDAPGPEWLAELERWLGAQPAPAPDDVRRA
                     LSARVPEYRPAPA"
BASE COUNT          364 a         1011 c         1006 g          373 t
ORIGIN      
        1 catcggcgac ctggtgtgca ccacgccgct cgtcgcggcg ctgcggcgcg cgctgcccga
       61 cgcgttcatc gccgccctcg tgaactcgta caaccgcgag gtgctcgacg gcaatcccga
      121 tgtcgatcgc gtgtacgcct acggcaaggc gaagcaccgc tcggggcgcg agtcgctgct
      181 cggcacctac ctgcgccgcg cggagctcgt gctcgagctg cggcgcgagc gcttcgacta
      241 cgccgtcctc gcggcgccgg gattccagcc gcgctcgctg cggctcgcgc ggctcgccgg
      301 cgcgcgccac gtcgtcggtt tcaccgaggg gcgcgctcgc gccgagcgcc gcgtcgactg
      361 cgccgttcct tatggcgcgc cggccgcgcg tcacgaggtc gaggacgtgc tgcggctgct
      421 ggggccgctc gggatctcgg cgcccgagcc gcggctgcgc gtcgtgcccg accccgggct
      481 cgccgagacg atgcgcgcga acgtcgccca acgcatgccc ggcggcgggc cgctggtcgc
      541 gctgcatatc agtgcgagga agcccagcca gcgctggccg gccgagcgct tcgctgcgct
      601 cgcgcgcgcc ttgtacgagc gcgccagcgc gcagcacgag cgcccgcgct tcgtcctgct
      661 gtgggctccg gggagcgagc gcaatcccac acatcccggc gacgacgaga aggcgcgcgc
      721 gctcgtcgcg atgctcgagg gcctgcccgc gctgccggtg ccgacgttcc ggctcgccga
      781 gctgatcgcc gccctcgacg cctgcgacgc ggtcgtatgc tccgacggcg gagcgatgca
      841 tctcgcggca gcgctcggca agccgatcgt gtgcttcttc ggcgactcgc agccctcgcg
      901 ctggcgaccg tggggcgtgc gccacgagct gctgcagccg gcgtcgcgcg acgcggtcga
      961 catcggcgtg gagcaggcgc tcgccgcgtt cgagcggctg cacgcgtcgg cgcgctaggc
     1021 gggcgccggc cggtattcgg ggacgcgcgc cgagagcgcg cggcgcacat cgtcgggggc
     1081 gggcgccggc tgcgctccca gccagcgctc gagctcggcc agccattcgg gccccggcgc
     1141 gtcgctcgag cgcgcgaccc gcagcatcgg gtgcggcgtc ggcagcgtct gctcctggtc
     1201 ggagatcggc tcctcgtaga gcttctcgcc cgggcgcagc ccgacgaact cgatgcgcac
     1261 gtcggtctcg gagaagcccg acaggcggat catctcgcgc gcgaggtcga cgatgcggat
     1321 cggcgtgccc atctcgagca cgtagatctc gccgccgcgt cccatccagc ccgcctgcag
     1381 cacgagctgc gcggcctcgg ggatcgacat gaaatagcgc gtgacctcgg gatgcgtcac
     1441 cgtgagcggg ccgccggccg cgatctgctc gcggaacttc gggatcacgc tgccggtgct
     1501 gccgatcacg ttgccgaagc gcaccgtgac gaacgaggtg gacgagccgc gctgcagcgc
     1561 gccgcacacc atctcggcga gccgcttggt cgcgcccatg acgttcgcag ggttcaccgc
     1621 cttgtcggtc gagaccagca cgaacttctc gacgccgctc gcctgcgcgg cgcgcgcgat
     1681 cgtcagcgtc ccgcggacat tgttctgcac cgcttcccag gcgttgtcgc gctccatgag
     1741 cggcacgtgc ttgtacgcgg cggcgtggaa cacgacctca gggcggacgc gaccgaggat
     1801 ctcggcgacc cgcgcggcgc tgcgcacgtc gccgatcacg cattcgatcg cgacctcggg
     1861 catgcgcgcg gcgaactcct gctcgatggt gtagagcgcg tactcgttca gctcgagcag
     1921 gacgagcagc ctcgggcgga agcgcgcgac ctggcgcgag agctcggcgc cgatcgagcc
     1981 gcccgcgccg gtgaccagca ccgtgctgcc ctcgagccag cccgagaggc ccgcgctgtc
     2041 caggcgcacc gggtcgcggc cgagcaggtc gtcgagctcg atgttgcgga tctgcgagat
     2101 cgtcaccttg ccgccgacga gatcgtcgaa cgacggcacg gtcatcacgc gcagcccggc
     2161 cgcggcgcac aggtcggccg cgcggcgccg gatggcgtgc gcgaccccgg gcatggcgat
     2221 gatcgcgtgc tcggcacctt cgcggcgcgc gtagcgcggc agcgcgtcga gcggcccgag
     2281 cacgcggatg ccgtggatgt cgcggccctg cttcaccggg tcgtcgtcga gcagcccgac
     2341 gatgcgccac tcctggctgc gcgcgagctc ggcgatcagc ttctcggcgg cctcgcccgc
     2401 gcccagcacc agcacggcct tgcgcgtgcc ggcgaggacg tcgcgcaggc ggtgctcgcg
     2461 caggtagcgg taggcgaggc ggctgcccga catggcgagc acgagcagca ccgggtcgag
     2521 gatgagcacc gagcgcggga cgagcagctc gggctgcgcc atgtagagca ccgtggcgac
     2581 gagcgccgcg gacaggccga cggcgagcgc gatgtgcttg aggtcggtga ggctcgcgaa
     2641 gcgccacatc ccgcggtaca gcccggagag caggaacacc accatctgca ccggcaccgc
     2701 ccactcgagc gagcgcagca ggctctcctg gaacggctcg gggatctcga agtt
//
LOCUS       DATDMW010000311         2192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2461796, whole genome shotgun sequence.
ACCESSION   DATDMW010000311 DATDMW010000000
VERSION     DATDMW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461796"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..396
                     /locus_tag="VI319_13145"
     CDS             148..396
                     /locus_tag="VI319_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864836.1"
                     /translation="MKKIHAALFAVLMLASASALAFHCPADMKKIDEALAKNPSLTPA
                     QLAEVKKQRAQGETLHKAGKHQESVEVLGKAMKTLHIQ"
     gene            complement(445..1395)
                     /gene="pip"
                     /locus_tag="VI319_13150"
     CDS             complement(445..1395)
                     /gene="pip"
                     /locus_tag="VI319_13150"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY6864837.1"
                     /translation="MTALYPEIEPRSSGMLRLDAVHSMYWEECGSPRGIPVVFLHGGP
                     GAGSSTKHRRLFDPAAYRIVVYDQRGSGRSTPLGELRDNTTPHLIADLETLRRHLGVE
                     RWLVFGGSWGSTLAIAYAEHHPERCLGLVLRGIFLCRRSEIDWFLYGLRNVYPEPWER
                     FAGFLPPDERSDLLVNYHRRLVDPDPAVHMPAARSWSVYEGSCSTLMPSDETVAYFAS
                     DGVALGLARMEAHYFMNDIFLPADSLLANAARLRRVPGIIVQGRYDMVCPAVTAHELH
                     AAWPEAVFRMVPDAGHSVWEPGIQSEVIEATERFRSRGSF"
     gene            complement(1400..>2192)
                     /locus_tag="VI319_13155"
     CDS             complement(1400..>2192)
                     /locus_tag="VI319_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY6864838.1"
                     /translation="NEGAGVVEAVGAGVSHVKAGDRVAYTGGPIGAYAEVRNMPADRL
                     VVLPEGISFETGAAMMLKGCTATYLLRQTYKVQRGDTILFHAAAGGVGLIACQMAKAI
                     GATVIGTVGSDDKAKLARAHGCDHTIVYTRENFVERVKALTDGKGVPVVYDGVGKDTF
                     MQSLDCLRPRGLMVSFGNASGAVGPTDLGILAAKGSLYVTRPTLNTYAAKREDLVELT
                     KELFDFVKSGKVRIEVNHKYPLKDAVQAHRDLESRKTTGSVIFTP"
BASE COUNT          351 a          770 c          742 g          329 t
ORIGIN      
        1 ctcgtggttc tggttctcga ctcaaggtcc cgctattcta tcggggccgc cggatgcgca
       61 ccggtccgtc catggaacac caacgggtgc gaccgtgttt accggacgcg gcgaaagccg
      121 tccctatcca ctgccaggag caacaacatg aagaagatcc acgcagccct gttcgcggtc
      181 ctgatgctgg cgagcgcctc ggcgctggcg ttccactgtc ccgcggacat gaagaagatc
      241 gacgaagcgc tcgcgaaaaa ccccagcctg acgccggccc agctcgccga ggtcaagaag
      301 cagcgcgccc agggcgagac gctgcacaag gccggcaagc accaggagtc ggtcgaggtc
      361 ctcggcaagg cgatgaagac gctgcacatc cagtagccgg aaccgccgcc ccgaacgccg
      421 cccccgggcg gcgtcgtttt cgcttcagaa cgacccgcgc gacctgaacc gctcggtcgc
      481 ctcgatcacc tcgctctgga ttcccggctc ccacaccgag tggcccgcgt cgggcaccat
      541 gcggaagacg gcctcgggcc acgcggcgtg cagctcgtgc gccgtgaccg ccgggcacac
      601 catgtcgtag cggccctgga cgatgatccc cgggacgcgg cgcaggcgag cggcgttggc
      661 gagcagcgaa tccgccggca ggaagatgtc gttcatgaag tagtgcgctt ccatgcgcgc
      721 gaggccgagg gcgaccccgt cgctcgcgaa gtacgcgacc gtctcgtcgc tcggcatcag
      781 cgtcgagcac gacccctcgt acacgctcca cgagcgcgca gccggcatgt gcaccgcggg
      841 atcgggatcg accaggcggc ggtggtaatt cacgaggagg tccgagcgct cgtcgggcgg
      901 caggaaaccc gcgaagcgct cccagggctc cggatagacg ttgcgcagcc cgtacaggaa
      961 ccagtcgatc tcgctccgcc ggcagaggaa gatgccgcgg agcacgagcc cgaggcagcg
     1021 ctcggggtga tgctcggcgt acgcgatcgc gagcgtgctg ccccacgatc cgccgaacac
     1081 cagccagcgc tccaccccca ggtggcggcg cagcgtctcg aggtcggcga tgagatgcgg
     1141 cgtcgtgttg tcgcgcagct cgccgagcgg cgtcgagcgc ccggacccgc gctggtcgta
     1201 gacgacgatg cggtaggcgg cggggtcgaa cagccgccgg tgcttggtcg agctgcccgc
     1261 gcccggtccg ccgtgcagga aaacgaccgg gatcccgcgc gggcttccgc actcctccca
     1321 gtacatcgag tggacggcat cgagccgcag catgccgctc gagcgcggct cgatctccgg
     1381 gtagagcgcg gtcatccgct cagggagtga agatcaccga ccccgtggtc ttgcgcgact
     1441 cgaggtcgcg gtgggcctgc accgcgtcct tcagcgggta tttgtggttc acctcgatcc
     1501 tgaccttgcc cgacttcacg aaatcgaaca gctccttcgt cagctcgacc aggtcctcgc
     1561 gcttcgcggc gtaggtgttc agggtcggcc gcgtgacgta gagcgagccc ttcgcggcga
     1621 gaatgccgag gtcggtcggg cccacggcgc ccgaggcgtt gccgaagctg accatcagcc
     1681 cgcgcggccg caggcagtcg agcgactgca tgaaggtgtc cttgccgacc ccgtcgtaga
     1741 cgaccgggac gcccttgccg tcggtcagcg ccttcacgcg ctcgacgaag ttctcgcgcg
     1801 tatacacgat cgtgtggtcg cagccgtgtg cgcgcgcgag cttcgccttg tcgtcggagc
     1861 cgaccgtgcc gatgacggtg gcgccgatcg ccttcgccat ctggcacgcg atcaggccga
     1921 cgccgcccgc cgcggcgtgg aagaggatgg tgtcgccgcg ctgcaccttg taggtctgcc
     1981 gcagcaggta cgtcgcggtg cagcccttca gcatcatcgc ggcgccggtc tcgaacgaga
     2041 tgccctcggg cagaacgacc agccggtcgg cgggcatgtt gcgtacctcg gcgtaggcgc
     2101 cgatcgggcc tccggtatag gcgacccggt cgcccgcctt cacgtggctg acgcccgccc
     2161 ccaccgcctc gaccacgcct gcgccttcgt tg
//
LOCUS       DATDMW010000312         7538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1090213, whole genome shotgun sequence.
ACCESSION   DATDMW010000312 DATDMW010000000
VERSION     DATDMW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090213"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="VI319_13160"
     CDS             <1..1825
                     /locus_tag="VI319_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY6864839.1"
                     /translation="QLRLSSGDAKAGSSAATVAAADDAAAKDKALKEANERVAMLEKN
                     VADMQRLLALKSGAGAQAQQQAEAAKAAAAPAPKAAPAPTPAVAAQPAPAPAPAAAKA
                     PDPVPAAKAPTQVAQATPPAAAPKAPEAAKAPEAQKATEAPKAPEAAKAPEAPKAVAA
                     APKAAPKAAPKTAPPEPDMLDQALALGEEYWMHGTGIMVVLLGYAAYAWRKKKREAGA
                     ATELSGAPSSDANSVFGTSGGQSIDTGSSSFQSDFSQSGIGKIDTEEIDPIAEADVYM
                     AYGRDAQAEEILKEALAKDSGRPAIRLKLLEIYANRKDTKAFQDGVSELQSITGGQGA
                     EWDKAMSLGRGIDPQNAMYGGAGGGPDVTMPDVAAAPAAPVMPDLVLPSTEAPAAALD
                     FNLDLGSGTPAEQTPDIALDAEPAAAASAPAGLDFDLGLGGDRAAQEKSDFSPGGTLI
                     IDAALAPASAEAAPAGADAPLNFDLELPGTPAAAPAAVAAPAAPPAAAPAADGMIEFD
                     LGGLPGAAPDPQAEPAKAPAIDLSAISLDIGAEPADAAPAGGAPDPRWQEVATKLDLA
                     KAYDEMGDKDGARELLKEVVKEGDTAQQQRAQSMLQALGAA"
     gene            1895..2677
                     /gene="truA"
                     /locus_tag="VI319_13165"
     CDS             1895..2677
                     /gene="truA"
                     /locus_tag="VI319_13165"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY6864840.1"
                     /translation="MRIAIGVAYRGTGFEGWQTQPSRRAVQDALERALAEIAGSPVTL
                     TAAGRTDAGVHASAQVAHFDTQAERPDTAWVRGANALLPETVSVQWAVRVEDDFHARY
                     SATGRSYRYVLYRDPVRPALLSGLVGWFHGELDAEAMRRALRPLLGEHDFSAFRSSEC
                     QAKSPVRVLDRAEISVNGPYLLLDFAANGFLHHMVRNIVGCLVYVGNGRQPPEWVAEL
                     MAKRDRRLAAPTFAPDGLYLAGVRYDARWKLPEFTRMMPFDA"
     gene            2674..3300
                     /locus_tag="VI319_13170"
     CDS             2674..3300
                     /locus_tag="VI319_13170"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY6864841.1"
                     /translation="MSTRTRVKICGITRVEDAQAAAAHGADAIGLIFYRPSPRYVTPD
                     RARAVVEALPPFVTPVAVFVNPSRGEVEGAIAAGAALLQFHGDEDPAFCAGFERPYIK
                     AARIRPGLNLLEYLAPYTTARGWMLDAYHDELWGGTGGAFDWSLVPARAERPIILSGG
                     LTAANVGEAVRRVRPWGVDVSTGVESSKGLKDAAKIAAFIAGVRNADG"
     gene            3293..4486
                     /gene="trpB"
                     /locus_tag="VI319_13175"
     CDS             3293..4486
                     /gene="trpB"
                     /locus_tag="VI319_13175"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY6864842.1"
                     /translation="MVDLPDERGHFGPYGGVFVAETLVHALDELREAYERARDDAEFQ
                     AEFADELKHYVGRPSPVYHAKRLSAKAGGAQIYIKREDLNHTGAHKINNTIGQALLAR
                     RMGKRRVIAETGAGMHGVAAATVAARYGMECVVYMGSEDVKRQVQNVYRMKLLGATVV
                     PVDSGSKTLKDALNEAMRDWVTRVEDTFYIIGTCAGPHPYPMMVRDFQSVIGRECIGQ
                     MREQAGRQPDAVIACVGGGSNAIGIFHPYIADASVRLIGVEAGGLGLSTGKHAASLVA
                     GKPGVLHGNRTYLLQDANGQIVETHSISAGLDYPGVGPEHAWLKDSGRAEYVTIDDAE
                     ALAAFHDLCRLEGIIPALESAHAIAYATKLAPTLPKDAILLVNLSGRGDKDMHTVAEK
                     SGIRF"
     gene            4483..5298
                     /locus_tag="VI319_13180"
     CDS             4483..5298
                     /locus_tag="VI319_13180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016727018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY6864843.1"
                     /translation="MNAPVFGGARLFNEDFFDAISRVRDASVDLVVADPPYGLGKDYG
                     NDSDRLSGADYLDFSRRWTDAVIPKLKPSGSLYVFLTWQNSPEVFSHLKTRMLMINEI
                     IWDRKVPSMGGSTRKFSSVHDTIGLFALSRDYYFDLDSVRIAYDPATKKARTRSIFVG
                     KKWLEVGYNPKDVWSETRLHRQDPEREDHPTQKPLKIVERMVLASCPPGGLVLDPFMG
                     SGTTAVAALKHGRGFVGFELNRRYFATARRRVSRLEHRAFEAPAASAPAQAAL"
     gene            5298..6113
                     /gene="trpA"
                     /locus_tag="VI319_13185"
     CDS             5298..6113
                     /gene="trpA"
                     /locus_tag="VI319_13185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY6864844.1"
                     /translation="MSRIQATFERLARERRKALIPYVTAGDPDPAATVPILHAMVEAG
                     ADILEVGVPFSDPMADGPVIQRAGERALRHGTGLPQVLAMAREFRRRDDRVPLVLMGY
                     ANPIEAMGVERFVEEADDAGVDGAIVVDYPPEECAPLADAMRRGGMDLVFLLAPTSTA
                     ERVAAVAKVASGYIYYVSLKGITGATHLDLADVEKNIARIRKATRLPIGVGFGIRDGA
                     TARAICEFADAAVIGSRIVQEVESAAPGQAAQSVGAFLRGVRRAMDEARKVAS"
     gene            6110..6979
                     /gene="accD"
                     /locus_tag="VI319_13190"
     CDS             6110..6979
                     /gene="accD"
                     /locus_tag="VI319_13190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY6864845.1"
                     /translation="MSWLQKLLPPKINRTGTGARKTVPEGLWTKCDSCAAVLYATDLE
                     KNASVCPKCGYHNRVSARTRLDLLLDPEGRFEIGAEVVPVDSLKFRDSKRYPERIEEA
                     EKETGETDALIVMQGAIRNTPVVAAAFEFEFMGGSMGSVVGERFVRGVHACLDQNLPL
                     ICITATGGARMQEGLLSLMQMAKTTAALTLLSQRKLPFITVLTDPTMGGVSASFAMLG
                     DIVIAEPRALIDFAGPRVIEQTVREKLPEGFQRAEFLLEKGAIDMIVDRRQMRDKLAS
                     LITLLQRQPGPAS"
     gene            7048..>7538
                     /locus_tag="VI319_13195"
     CDS             7048..>7538
                     /locus_tag="VI319_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY6864846.1"
                     /translation="MGLERVREVRERLGSDTGAVLIVVGGTNGKGSACALLEAISLAA
                     GYRVGLYTSPHLLRYNERVRIGGRPVGDQRLIAGFESVERARAHEGREVPLTYFEFGT
                     LAAWEIFCAEALDVVILEVGLGGRLDAVNVFDADCAVLTSIGIDHTDYLGPTRESIGA
                     EKAG"
BASE COUNT         1208 a         2650 c         2670 g         1010 t
ORIGIN      
        1 tcaattgcgg ctgtcgagcg gggacgcgaa ggcgggcagc agcgccgcca cggtcgccgc
       61 cgccgacgac gcggcggcca aggacaaggc gctcaaggaa gcgaacgagc gcgtcgcgat
      121 gctcgagaag aacgtcgccg acatgcagcg gttgctcgcg ctgaagagtg gcgccggcgc
      181 ccaggcgcag cagcaggccg aggccgccaa agcagctgcc gcgcccgccc cgaaagccgc
      241 cccggcgccg acgcccgcgg ttgcggcgca gccggctccg gctcccgccc cggccgcggc
      301 gaaggccccg gacccggtac cggccgcgaa agccccgacc caggtcgccc aggcaacgcc
      361 gccggcggcc gcacccaagg ccccggaagc agccaaagct ccagaggcgc agaaggcaac
      421 cgaagcgccc aaggcgcccg aggccgcgaa agcccccgaa gcgcccaaag cggtcgccgc
      481 cgccccgaag gccgccccga aggccgcgcc gaagacggcg cctcccgagc ccgatatgct
      541 ggaccaggcg ctggcgcttg gggaggaata ctggatgcac ggtaccggca tcatggtcgt
      601 gctgctcggt tacgccgcct acgcctggcg aaaaaaaaaa cgtgaagcgg gcgccgcgac
      661 ggagctgagc ggcgcgccga gctccgacgc caactctgtt ttcgggacgt cgggcggcca
      721 gagcatcgat accggctcga gctcgttcca gagcgacttc agccagagcg gcatcgggaa
      781 gatcgacacc gaggagatcg acccgatcgc cgaggccgac gtgtacatgg cctacggccg
      841 cgacgcgcaa gccgaggaaa tcctcaagga agcgctcgcc aaggattcgg gccgccccgc
      901 gatccgcctg aagctgctcg agatctacgc caaccgcaag gacaccaagg cgttccagga
      961 cggcgtgagc gagttgcagt cgatcaccgg cggccaggga gccgagtggg acaaggcgat
     1021 gagcctcggg cgcggcatcg acccgcagaa cgcgatgtac ggcggcgcgg gcggcggtcc
     1081 cgatgtcacg atgcccgatg tcgccgccgc gccggcggcg cccgtgatgc ccgacctggt
     1141 gctgccgagc acggaggcac ctgcggcggc gctcgatttc aacctcgatc tgggcagcgg
     1201 gacgcccgcc gagcagacgc ccgacatcgc gctcgacgcc gagcccgcgg cggccgcctc
     1261 ggcgccggcg ggcctcgatt tcgacctggg cctgggcggt gacagggcgg cacaggagaa
     1321 gagcgacttt tctccgggcg gcacgttgat catcgacgcg gcgctcgcgc ccgcttcggc
     1381 cgaggctgcc cccgcgggcg ccgacgcccc gctcaatttc gatctcgagc ttcccggaac
     1441 gcctgccgcc gcgccggctg ccgtcgccgc gcccgcagcg ccgcccgccg cggcgcccgc
     1501 agcggacggc atgatcgagt tcgacctcgg cggcctgccg ggcgcagcgc cggatccgca
     1561 agccgagccc gcgaaggcgc cggcgatcga cctctcggcg atcagcctgg atatcggggc
     1621 cgagcccgcc gacgccgctc ctgccggagg ggccccggac ccgcgatggc aggaggtcgc
     1681 gaccaagctc gacctcgcca aggcctatga tgagatgggc gacaaggacg gtgcgcgcga
     1741 gctgctgaag gaggtcgtga aggaaggcga cacggcgcag cagcagcggg cgcagagcat
     1801 gctccaggcg ctcggcgccg catagcgctc cgcgagcgtc ccatcgaatc ggcgccgacg
     1861 gcgccgattt cgtttttcga ggaccgatac gaacatgcgc atcgcgatag gcgtcgcata
     1921 ccgcggcacc ggcttcgagg gctggcagac ccagccctcg cgcagggccg tgcaggacgc
     1981 actcgagcgc gcactcgccg agatcgccgg ctcgccggtg acgctgaccg cggcgggccg
     2041 caccgacgcc ggggttcacg cgagcgcgca ggtcgcgcac ttcgatacgc aagccgagcg
     2101 tcccgacacg gcctgggtgc gcggcgcgaa cgcgctgctt ccggagacgg tctcggtgca
     2161 gtgggcggtg cgcgtcgagg acgacttcca cgcgcgctac tcggccaccg ggcggagcta
     2221 ccgctacgtg ctgtatcgcg atcccgtgcg gcccgcgctg ctctcggggc tggtcggctg
     2281 gttccatggc gagctcgacg ccgaggcgat gcggcgcgcg ctgcggccgc tgctcggcga
     2341 gcacgacttc tcggcgttcc gctcgtcgga atgtcaggcg aagagcccgg tgcgcgtgct
     2401 cgaccgcgcc gagatctcgg tgaacggccc gtatctgctg ctcgacttcg ccgccaacgg
     2461 gttcctgcac cacatggtgc gcaacatcgt cggttgcctg gtctacgtcg gcaacggccg
     2521 gcagccgccg gagtgggtcg cggagctgat ggcgaagcgc gaccgccggc tcgccgcgcc
     2581 gaccttcgcg cccgacgggc tgtacctcgc gggtgtgcgc tacgacgcgc gttggaagct
     2641 gcccgagttc acccgtatga tgcccttcga cgcatgagca cgcgcacccg ggtcaagatc
     2701 tgcggcatca cgcgcgtcga ggacgcgcaa gcggcggccg cgcacggcgc ggacgcgatc
     2761 ggcctcatct tctaccggcc gagtccccgt tatgtgacgc cggatcgcgc gcgcgccgtc
     2821 gtcgaggcgc tgccgccgtt cgtgacgccg gtcgcggtgt tcgtgaatcc ctcgcgcggt
     2881 gaggtcgagg gcgcgatcgc agcgggcgcc gcgctgctgc agttccacgg cgacgaggac
     2941 ccggcgttct gcgccggctt cgagcggccc tacatcaagg cggcgcggat tcgtccgggg
     3001 ttgaatttgc tagaatatct ggcgccttac acgaccgccc ggggctggat gctcgacgcc
     3061 taccacgacg agctgtgggg cggcaccgga ggcgcgttcg actggagcct cgtgccggcg
     3121 cgcgccgagc ggccgatcat cctttcgggc ggcctgaccg cggcaaacgt cggcgaagcc
     3181 gtgcggcgcg tccggccctg gggcgtggac gtgtcgaccg gcgtggaatc gtcgaaaggc
     3241 ttgaaggacg ccgcgaagat cgcggcgttt atcgcaggag tccggaatgc ggatggttga
     3301 cctgcccgac gagcgcgggc acttcggtcc gtacggcggc gtcttcgtcg ccgagacgct
     3361 ggtgcacgcg ctcgacgagc tgcgcgaggc gtacgagcgt gcgcgcgacg acgccgagtt
     3421 ccaggccgag ttcgcggacg agctcaaaca ttatgtgggc cgcccgagcc ccgtttatca
     3481 cgccaagcgc ctctccgcga aggccggggg cgcgcagatc tacatcaagc gcgaggacct
     3541 gaaccacacc ggcgcgcaca agatcaacaa caccatcggc caggcgctgc tcgcgcggcg
     3601 catgggcaag cgccgcgtga tcgcggagac cggcgccggg atgcatggcg tcgccgcggc
     3661 caccgtggcg gcgcgctacg gcatggaatg cgtcgtgtac atgggcagcg aggacgtgaa
     3721 gcgccaggtg cagaacgtct accgcatgaa gctgctcggc gcgacggtcg tgccggtcga
     3781 ctcgggctcg aagacgctca aggacgcgct gaacgaggcg atgcgcgact gggtgacgcg
     3841 cgtcgaggac accttctaca tcatcggcac ctgcgccggg ccgcatccgt acccgatgat
     3901 ggtgcgcgac ttccagtcgg tgatcggccg cgagtgcatc gggcagatgc gcgagcaggc
     3961 cgggcggcag cccgacgccg tgatcgcctg cgtcgggggc ggctcgaacg cgatcggcat
     4021 tttccatccg tacatcgccg acgcgtcggt gcggctgatc ggcgtcgagg cgggcgggct
     4081 gggcctctcg accggcaagc acgccgcgtc gctcgtcgcg ggcaaacccg gcgtcctgca
     4141 cggcaaccgc acctacctgc tccaggacgc gaacggccag atcgtcgaga cgcactcgat
     4201 ctcggcgggg ctcgactatc ccggcgtcgg ccccgagcac gcgtggctga aggactcggg
     4261 gcgcgccgag tacgtcacca tcgacgacgc cgaggcgctc gccgcgttcc acgacctgtg
     4321 ccggctcgag ggcatcatcc cggcgctcga atccgcgcac gcgatcgcct atgcgacgaa
     4381 gctcgcgccg accctgccga aggacgcgat cctcctcgtc aacctctcgg gacgcggcga
     4441 caaggacatg cacaccgtcg ccgagaagtc gggaatcagg ttttgaacgc gcccgtcttc
     4501 ggcggcgcgc gcctgttcaa cgaggatttc ttcgacgcga tctcgcgcgt gcgcgatgcg
     4561 agcgtcgacc tcgtggtcgc cgatccgccc tacggcctgg gcaaggacta cggcaacgac
     4621 tccgaccggc tgagcggcgc cgactatctc gacttcagcc gccgctggac cgacgcggtg
     4681 atcccgaagc tcaagccctc cggcagcctc tacgtgttcc tcacctggca gaacagcccc
     4741 gaggtcttca gccatctgaa gacgcgcatg ctgatgatca acgagatcat ctgggaccgg
     4801 aaagtcccga gcatgggcgg gagcacccgc aagttctcct cggtgcacga cacgatcggg
     4861 ctgttcgccc tgtcgcgcga ctactatttc gacctcgaca gcgtgcgcat cgcctacgat
     4921 ccggcgacca agaaggcgcg cacgcgctcg atcttcgtcg gcaagaaatg gctcgaggtc
     4981 gggtacaacc ccaaggacgt gtggagcgag acgcgcctgc accgccagga ccccgagcgc
     5041 gaggaccatc cgacgcagaa gccgctcaag atcgtcgagc gcatggtgct cgcgagctgt
     5101 ccgcccggcg gactggtgct cgatccgttc atggggagcg gcaccaccgc cgtcgcggcg
     5161 ctgaagcacg gccgcggctt cgtcggtttc gagctgaacc gccgctattt cgcgacggcc
     5221 aggcgccgcg tgtcgcggct cgagcaccgg gccttcgagg cgcccgccgc gagcgcgccg
     5281 gcgcaggcgg cgctctgatg tcgcgcatcc aggccacctt cgagcggctc gcgcgcgagc
     5341 gccgcaaggc gctcatcccg tacgtgacgg cgggcgatcc ggatcctgcg gcgacggttc
     5401 ccatcctgca cgcgatggtg gaagccggcg cggacatcct ggaggtgggc gtgccgtttt
     5461 ccgacccgat ggccgacggc cccgtgatcc agcgcgcggg cgagcgcgcg ctcaggcacg
     5521 gcaccggcct gccgcaggtg ctcgccatgg cgcgcgagtt ccgccgccgc gacgaccgcg
     5581 tgccgctcgt gctcatgggc tatgcgaacc cgatcgaggc catgggcgtc gagcgcttcg
     5641 tcgaggaagc cgatgacgcg ggggtcgacg gcgcgatcgt cgtcgactat ccgcccgagg
     5701 agtgcgcgcc gctcgccgac gcgatgcgcc ggggcggcat ggacctcgtg ttcctgctcg
     5761 cgccgacctc cacggccgag cgcgtcgccg cggtcgcgaa ggtcgcgagc ggatacatct
     5821 actacgtctc gctgaagggc atcacgggcg cgacgcacct cgacctcgcc gacgtcgaga
     5881 agaacatcgc gcgcatccgc aaggcgacga ggctgccgat cggcgtcggg ttcggcatcc
     5941 gcgacggcgc cacggcgcgc gcgatctgcg agttcgccga cgccgccgtc atcggcagcc
     6001 gcatcgtgca ggaagtggag agcgcggcgc ccggacaggc cgcgcagagc gtcggcgcgt
     6061 tcctgcgcgg cgtgcgccgg gcgatggacg aagcgcggaa ggtggcctcg tgagctggct
     6121 gcagaagctg ctcccgccga agatcaaccg caccggcacg ggcgcgcgca agaccgtgcc
     6181 cgagggcctg tggacgaaat gcgattcgtg cgcggccgtg ctgtacgcca cggatctcga
     6241 gaagaacgcc agcgtctgcc cgaaatgcgg ctaccacaac cgcgtcagcg cacgcacacg
     6301 cctcgacctg ctcctcgacc cggaaggccg cttcgagatc ggcgccgagg tcgtgccggt
     6361 cgacagcttg aagttcaggg acagcaagcg ctatcccgag cgcatcgagg aggcggagaa
     6421 ggagacgggc gagaccgacg ccctgatcgt catgcagggc gcgatccgga acacgcccgt
     6481 ggttgcggcc gcgttcgagt tcgagttcat gggcggatcg atgggctcgg tggtcggcga
     6541 gcgcttcgtg cgcggcgtgc acgcctgcct ggaccagaac ctgcccctga tctgcatcac
     6601 cgccacgggc ggcgcgcgca tgcaggaagg gctgctgtcg ctcatgcaga tggcgaagac
     6661 caccgcggcg ctgacgctgc tctcgcagcg caagctgccg ttcatcaccg tgctcacgga
     6721 cccgaccatg ggcggcgtgt cggcgagctt cgccatgctc ggcgatatcg tgatcgcaga
     6781 gccgcgcgcc ctgatcgact tcgccggccc gcgggtcatc gagcagaccg tgcgcgagaa
     6841 gctgcccgaa ggcttccagc gcgcggagtt ccttctggag aagggcgcca tcgacatgat
     6901 cgtcgaccgg cgccagatgc gcgacaagct ggcgagcctg atcacgctgc tgcagcgcca
     6961 gccgggaccc gcgagctgag cgcgaccgca acagaccttt ccgggtggct cgagtacatc
     7021 gagcgcatcc acccgaagac catcgagatg ggcctcgagc gcgtgcgcga ggtgcgcgaa
     7081 cggctcggca gcgacaccgg cgcagtgctc atcgtcgtcg gtggcaccaa cggcaaaggc
     7141 tccgcctgcg cgctgctcga ggcgatctcg ctcgcggcgg gctaccgcgt cggcctctac
     7201 acctcgccgc atctgctgcg ctacaacgag cgcgtgcgca tcggcgggcg gcccgtcggc
     7261 gatcagcggc tgatcgccgg cttcgagagc gtcgaacgcg cccgcgcgca cgaagggcgc
     7321 gaggtgccgc tcacctactt cgaattcggg acgctcgccg cctgggagat cttctgcgcc
     7381 gaggcgctcg acgtcgtcat cctggaggtc ggcctcggcg gacggctgga cgcggtcaac
     7441 gtcttcgacg ccgactgcgc ggtcctgacc agcatcggca tcgaccacac cgactatctc
     7501 gggccgacgc gcgagagcat cggcgcggag aaggccgg
//
LOCUS       DATDMW010000313         3354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_467657, whole genome shotgun sequence.
ACCESSION   DATDMW010000313 DATDMW010000000
VERSION     DATDMW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467657"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VI319_13200"
     CDS             complement(<1..970)
                     /locus_tag="VI319_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6864847.1"
                     /translation="MNAPETKPRKAEFSWQDPLRFDAQLSDDERMVRDAAAKYAKDKL
                     APRVLEAFRHEKSDPAIFREMGELGLLGPTIPAEYGGAGMSYVCYGLIAREIERVDSG
                     YRSMLSVQSSLVMLPIHRFGSEAHRKKYLPKLATGEMIGCFGLTEPNHGSDPGSMQTR
                     AKKVPGGYSLTGSKMWISNSPIADLFLVWAKTEDGKIRGFLLEKGAKGLSAPAIHGKM
                     GLRTSITGEVVLDDVRVPEDALMPRVEGLKGPFTCLNAARYGIAWGALGAAEFCWQAA
                     RQYTLDRKQFGRPLAANQLIQKKLADMQTEIALGLQGCLRLGRLKDE"
     gene            complement(984..2504)
                     /locus_tag="VI319_13205"
     CDS             complement(984..2504)
                     /locus_tag="VI319_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY6864848.1"
                     /translation="MPLDAARADLTLAVVGAGVMGRGIAQLFAQAGIRVLLHDARAGA
                     ADEARREVAKQFDRLAEKGRMQRADADRAASRIEVAGAPAGLAPAHGVVEAIVESLDA
                     KRALFRELEAIVAEDCLLASNTSSLSVTAIASACRRPERVGGFHFFNPVPLMKVVEVV
                     DGLLTAPWVGDSLAAIAARCGHRAVRAKDTPGFIVNHVGRGYGTEALRVAGEGVAGFH
                     EIDRILREAAGFRMGPFELLDLTGLDVSHPVMESIYRQYYDEPRYRPSPITAQRLAAG
                     VLGRKSARGFYAYADGKPSTEPEDPAPAARPARVWISRAGAGMKLGELLQKLGAPLDP
                     NARPAAQSLCLVAPLGQDATTTALEEGLDPRRTLAVDCLFPMEKRRTLMTTPVTSPTM
                     REAAHGLLGTDGVPVSVIRDSAGFVAQRVIATVVNIACDVAQQRIASPADIDAGATLG
                     LGYPQGPLAWGDALGPATLLRILEAMQAFYGDPRYRPSPWLKRRALLGVSLLTPEA"
     gene            2638..>3354
                     /locus_tag="VI319_13210"
     CDS             2638..>3354
                     /locus_tag="VI319_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864849.1"
                     /translation="MNGLARLLPLLALALVWPAAAQDFPSRPLTLVCPWPAGGSTDTH
                     LRRFSEISAKYLGQPMVVENRPGNGGMIGALHVARTARPDGYTLSQLTIAAFRFPHMQ
                     KVDWDPIRDFTYVIGVSGYTFGVVVRSDSPIQSFRELVAWAKANPGKLSYASTGTGTS
                     PHLLMEEVAARQGIELLHVPFKGSADATQALLGAHVMALADSSGWGRFVDAGQFRLLV
                     TFGAARTKHWPSVPTAKDLGM"
BASE COUNT          456 a         1191 c         1207 g          500 t
ORIGIN      
        1 cttcgtcctt gagtcgcccc aggcgcaggc agccctggag gccgagcgcg atctccgtct
       61 gcatgtcggc gagcttcttc tggatgagct ggttcgccgc gagcggccgc ccgaactgct
      121 tgcgatcgag cgtgtactga cgcgccgcct gccagcagaa ctcggcggcg ccgagcgcgc
      181 cccacgcgat gccgtagcgc gcggcgttca ggcaggtgaa cggacccttc aggccctcga
      241 cgcgaggcat gagcgcgtcc tcgggcacgc gcacgtcgtc gagcacgact tctcccgtga
      301 tcgaggtgcg caagcccatc ttgccgtgga tcgccggggc ggagaggccc ttcgcgcctt
      361 tctcgagcag gaagccgcgg atcttgccgt cctcggtctt cgcccagacc aggaacaggt
      421 ccgcgatcgg cgagttgctg atccacatct tcgagccagt gaggctgtag ccgcccggga
      481 ccttcttcgc gcgggtctgc atgctgccgg gatcggagcc gtggttgggc tcggtgaggc
      541 cgaagcagcc gatcatctcg cccgtcgcga gcttcggcag atatttcttc cggtgcgcct
      601 cgctgccgaa gcggtggatc ggcagcatga cgagcgagct ctgcaccgag agcatcgagc
      661 ggtaacccga gtcgacgcgc tcgatctcgc gcgcgatcag gccgtagcag acgtagctca
      721 tgccggcgcc gccgtactcg gccgggatcg tgggcccgag caggcccagc tcgcccatct
      781 cgcgaaagat cgcgggatcg ctcttctcgt ggcggaacgc ctcgagcacg cgcggcgcga
      841 gcttgtcctt cgcgtacttg gcggcggcgt cgcgaaccat gcgctcgtcg tcggagagct
      901 gcgcgtcgaa gcgcagcggg tcctgccacg agaactcggc cttcctgggt ttggtttcgg
      961 gggcgttcat gccggttctc gcttcaggct tcgggagtga gcaatgatac gccgagcagc
     1021 gcgcggcgct tcagccaggg gctcggccgg tagcgcgggt cgccgtagaa ggcctgcatc
     1081 gcttcgagga tacgcagcag cgtggcgggc cccagcgcgt cgccccaggc gagcgggccc
     1141 tgcgggtagc cgaggccgag cgtcgccccc gcgtcgatgt ccgcgggcga ggcgatgcgc
     1201 tgctgcgcga cgtcgcaggc gatgttgacg acggtcgcga tgacgcgctg cgcgacgaat
     1261 ccggcgctgt cgcggatcac cgagaccggc acgccgtcgg tgccgagcag cccgtgcgcg
     1321 gcctcgcgca tcgtcgggct cgtgaccggg gtcgtcatca gcgtgcgccg cttctccatc
     1381 gggaacaggc agtcgacggc gagcgtgcgc cgcggatcga gcccctcctc gagcgcggtc
     1441 gtggtcgcgt cctggccgag cggcgcgacc aggcagagcg actgcgccgc cgggcgcgcg
     1501 ttggggtcca atggagcgcc gagcttctgt agcagctcgc cgagcttcat gcccgcgccc
     1561 gcgcggctga tccagacgcg agccgggcgc gccgcgggcg ccggatcctc gggctccgtg
     1621 ctcggcttgc cgtccgcgta cgcgtagaac ccgcgcgccg acttgcgccc gagcacgccc
     1681 gccgcgagcc gctgcgccgt gatcggcgag ggccggtagc gcggctcgtc gtagtactgg
     1741 cggtagatcg actccatgac gggatgcgaa acatcgaggc ccgtgaggtc gagcagctcg
     1801 aacggcccca tgcggaagcc ggccgcctcg cgcaggatgc ggtcgatctc gtggaagccg
     1861 gcgacgcctt ctcccgcgac gcgcagcgcc tcggtgccgt agccgcgccc gacgtggttg
     1921 acgatgaagc ccggcgtgtc cttcgcgcgc accgcgcggt ggccgcagcg cgcggcgatc
     1981 gcggcgagcg agtcgccgac ccagggcgcg gtcagcaggc cgtcgacgac ctcgaccacc
     2041 ttcatgagcg gcaccgggtt gaagaaatgg aagccgccga cccgctccgg acgccggcag
     2101 gccgacgcga tcgccgtgac ggagagggaa gacgtgttgc tcgcgagcag gcagtcctcg
     2161 gcgacgatcg cctcgagctc gcggaacagc gcgcgcttcg cgtcgagcga ctcgacgatc
     2221 gcctcgacca cgccgtgcgc gggcgcgagc ccggcgggcg cgccggcgac ctcgatgcgg
     2281 ctcgcggcgc ggtcggcgtc ggcgcgctgc atccgcccct tctcggcgag gcggtcgaat
     2341 tgcttcgcga cctcgcggcg cgcctcgtcg gccgcgcccg cccgggcgtc gtggagcagc
     2401 acccggatcc cggcctgggc gaacagctgc gcaatgccgc gccccatgac cccggcgccg
     2461 acgactgcga gcgtgagatc ggcgcgggcg gcgtcgagcg gcatcgggtc gatgggcttg
     2521 gaaaggcgca gattctaacc cgcgccccga cgccggcgag cggccgggtc ctacccgttt
     2581 gacgtacatc aaccttttgg gcaagctgtg acccgccgcc gtggaggagg acccggaatg
     2641 aacggtctcg cccgcttgct tccgctgctc gcgctggcgc tcgtctggcc ggccgccgcg
     2701 caggatttcc cgtcgcgccc gctgacgctg gtctgcccgt ggcccgccgg cggctcgacc
     2761 gacacgcacc tgcgccgctt ctccgagatc tcggcgaaat acctcggcca gccgatggtg
     2821 gtcgagaacc gcccgggcaa cggcggcatg atcggcgccc tgcacgtcgc gcgcaccgcc
     2881 aggcccgacg gctatacgct gtcgcagctc accatcgccg cgttccgctt cccgcacatg
     2941 cagaaggtcg actgggaccc gatccgcgac ttcacctacg tcatcggcgt ctcgggctac
     3001 accttcggcg tggtggtgcg ctccgattcc ccgatccagt cgttccggga gctggtcgcg
     3061 tgggcgaagg ccaatcccgg caagctctcg tacgcgtcga ccggcacggg cacgtcgccg
     3121 cacctgctca tggaggaggt ggcggcgagg caggggatcg agctcctgca cgtgccgttc
     3181 aagggcagcg ccgacgcgac gcaggcgctg ctcggcgcgc acgtcatggc gctggcggac
     3241 tcgagcggct ggggccgctt cgtcgacgcc gggcagttcc gcctgctcgt gaccttcggc
     3301 gccgcgcgca ccaagcactg gccgagcgtg ccgacggcga aggacctcgg gatg
//
LOCUS       DATDMW010000314         3972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_841834, whole genome shotgun sequence.
ACCESSION   DATDMW010000314 DATDMW010000000
VERSION     DATDMW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841834"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VI319_13215"
     CDS             <1..618
                     /locus_tag="VI319_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864850.1"
                     /translation="KVDALLVQRGAGAAAMLPLRGVTRPDDIAREVAAAGGGSAVLLD
                     LKGESETMYRDYRREALAHSLLGALAITVLLSVTLRSAARVFDVLAPLVAAVVLTAAG
                     LLLVAGKLSMFHLVGLLLVVAVGSNYSLFFERRAASGHDRERTVVSLGFACLSTVIGF
                     GLLAFSRVPLLSAIGSTVGSGAVLALVFSAALARGGAPRPSPVHP"
     gene            615..1454
                     /locus_tag="VI319_13220"
     CDS             615..1454
                     /locus_tag="VI319_13220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6864851.1"
                     /translation="MTQAYAGSQTRANGARRPALALRLSALAHAAVALGIALHPDAWP
                     WGLAALTANYCALGVAVFFPRSALLGPNISRLPRAAIERGEVCITFDDGPHPDITPRV
                     LDILDRHGAKATFFCVGERVEAHPDIAREIARRGHTVESHSHRHAPVFALYGLRGLRR
                     EVQAAQRVIAQATGRDPVFFRAPAGFRSPLLDPVLVSLGLRYASWTRRGFDTADRNAG
                     RVLRRLTRGLAAGDILLLHDSVPRVLEVLPPLLDELRARSLKPVSLPGGCDDGCADDG
                     RDA"
     gene            1438..2145
                     /locus_tag="VI319_13225"
     CDS             1438..2145
                     /locus_tag="VI319_13225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6864852.1"
                     /translation="MDATLSLLREEASRPFLRAGRAAWHYARGKFRWDPVYFALLHSG
                     VLPDSGTLVDLGCGQGILLSLLSAAKEQYARGDWPADWPAPPARLAMHGYDLRRRSIR
                     AGSVALDGVAALECRDIRGLALPPADAVVILDVLYHLRYADQERVLDAVAHALRPGGV
                     LILRESNSRGGLAHRLQRGAEIIAQLWHGRLAPKLWYRDAAQWRTLLERRGMMVTTQP
                     MNGDTPFANVLFLARRR"
     gene            2210..3400
                     /locus_tag="VI319_13230"
     CDS             2210..3400
                     /locus_tag="VI319_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY6864853.1"
                     /translation="MQPLVISDFSIVNSLGADRDAVAHALRAMRSGLAPCDFETVRLE
                     TSIGRVAALDDLRVRADLAAYDCRNNRLAQLGLESGGFARAAAALREKYGAHRVGVFV
                     GTSTSGVLETELAYRRRDPATGALPTDYRYRETQNTGSVGDFARRYLGLRGPEVVISS
                     ACSSSAKAFGNAARMIAAGVCDAAIVGGVDSLCLTTLYGFHSLELTSRAPCRPFDAER
                     DGISIGEGAGFAIVEKPEARPARSHALLLGVGESSDAYHMSTPHPEGLGARTAMERAL
                     ASAGLAASDVDYINLHGTATRTNDASEDRAVRDLFGVGTPCSSTKGATGHLLGAAGIT
                     EAIVSILAIQGGFMPGSPGTRRIDPSLGSGYLIETRPAKLRRVLSNSFGFGGSNCSLV
                     LGAP"
     gene            3404..>3972
                     /locus_tag="VI319_13235"
     CDS             3404..>3972
                     /locus_tag="VI319_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY6864854.1"
                     /translation="MAMRAYVEGIAVLGPGLAGWEASRAVLAGTAPYRPEAAVVPPSD
                     LLPPAERRRTGLPVKLALAVGREAFLNAGRDASATRTVFTASCGDGQNLHDMCESLAR
                     PEREVSPTRFHNSVHNAAAGYWGIATRSREASTSLCAYDASFGAGLLEAMAQVAVDGV
                     AVALLAYDQPYPEPLHRARPIGGVFAAALV"
BASE COUNT          462 a         1481 c         1421 g          608 t
ORIGIN      
        1 aaggtcgacg cccttctcgt gcagcgcggc gctggcgcgg ccgccatgtt gccgcttcgc
       61 ggcgtgacgc gtcccgacga catcgcccgg gaggtcgccg ccgcgggcgg cggctccgcg
      121 gtgctgctcg acctgaaagg cgagtcggag accatgtatc gcgattaccg ccgcgaggcc
      181 ctcgcgcact cgctgctcgg cgcgctggcg atcacggtgc tgctgtcggt cacgctgcgc
      241 tcggcggcgc gcgtcttcga cgtgctcgcg ccgctcgtcg ccgcggtggt gctcaccgcg
      301 gcggggctct tgctcgtcgc cgggaagctg tcgatgtttc atctcgtcgg gctgctgctc
      361 gtggtcgcgg tgggctcgaa ctactcgctg ttcttcgagc ggcgcgcggc gtcgggacac
      421 gaccgcgagc gcaccgtcgt gtcgctcggg ttcgcgtgcc tgtcgacggt catcggcttc
      481 gggctgctcg cgttctcgcg ggtgccgctg ctgagcgcga tcggatccac cgtcgggtcc
      541 ggcgcggtcc tggcgctggt cttctccgca gcgctcgccc gcggcggcgc gccgcgcccg
      601 tcgccggtgc atccgtgacg caggcctacg ccggctcgca gacgcgcgcc aacggcgcgc
      661 ggcgccccgc gctcgcactg cgcctctcgg cgctcgcgca tgcggcggtg gcgcttggca
      721 tcgcgctgca ccccgacgcc tggccttggg gtctcgcggc gctgacggcc aactactgcg
      781 cgctcggcgt ggccgtcttc tttccgcggt cggcgctgct cgggccgaac atctcgcgct
      841 tgccgcgcgc cgcgatcgag cgcggcgagg tctgcatcac gttcgacgac ggcccccatc
      901 cggacatcac gccgcgcgtg ctcgacatcc tcgatcgcca cggagcgaag gcgacgttct
      961 tctgcgtcgg cgagcgggtc gaggctcatc ccgacatcgc gcgcgagatc gcgcgtcgcg
     1021 gccacacggt ggaaagccac agtcaccggc acgcgcccgt cttcgcgctc tacggcctgc
     1081 gcgggctgcg acgcgaagtg caggcggctc agcgcgtcat cgcgcaggcg accggccgcg
     1141 atccggtgtt cttccgcgcg cccgccggat tccgcagccc cttgctcgat ccggtgctcg
     1201 tctctctggg cctgcgctac gcgtcctgga cccggcgcgg cttcgacacg gccgaccgca
     1261 acgccgggcg cgtgctgcga cggctcacgc gcgggctcgc ggccggcgac attctgctgc
     1321 tgcacgacag cgtaccgcgc gtgctggagg tgctcccgcc gctgctcgat gagctgcgag
     1381 cgcgttcgct gaagccggtg tcgctgccgg gggggtgcga cgatggatgc gccgacgatg
     1441 gacgcgacgc ttagcctgct gcgcgaggag gcgagccggc cgttcctgcg cgccggccgc
     1501 gcggcgtggc actacgcgcg cgggaagttc cgctgggacc cggtttactt cgcgctgctg
     1561 cacagcggcg tgctgcccga ctccgggacg ctggtcgacc tcggctgcgg gcagggcatc
     1621 ctgctgtcgc tgctctccgc ggcgaaggag cagtatgcac gcggcgactg gccggcggat
     1681 tggcccgcgc cgcccgcgcg cctcgcgatg cacggctacg atctgcgccg ccgctcgatc
     1741 cgcgccggca gcgtcgcgct cgacggggtc gccgcgctcg aatgccgcga catccgcggc
     1801 ctggcgctgc cgccggccga cgccgtcgtc attctcgacg tcctgtatca cctgcgctat
     1861 gccgatcagg agcgggtgct cgacgcggtg gcgcatgccc tgcggccggg cggggtgctg
     1921 atcctgcgcg agtcgaattc ccgcggcggc ctcgcgcacc ggctgcagcg cggcgccgag
     1981 atcatcgcgc agctgtggca cggacggctc gcgcccaagc tctggtaccg cgacgccgcg
     2041 caatggcgga ccttgctcga gcggcgcggg atgatggtca cgacgcagcc gatgaacggc
     2101 gacacaccgt tcgcgaacgt gctgttcctc gcgaggcgtc gttgagccgc ctttttgata
     2161 aactcgagcc ggcgcccggc gcccgataga tcaccgaccg gcgatcccct tgcagcctct
     2221 cgtcatcagc gatttttcca tcgtcaactc gctcggcgcg gaccgcgacg cagtcgcgca
     2281 cgcgctgcgc gcgatgcgct cggggctcgc gccctgcgat ttcgagacgg tgcgcctgga
     2341 gacgagcatc gggcgcgtgg ccgcgctcga cgaccttcgc gtgcgcgccg acctcgcggc
     2401 ctacgattgc cgcaacaacc gtctcgcgca gctcgggctc gagtccggcg gcttcgcgcg
     2461 cgcggcggcg gcgctgcgcg agaagtacgg cgcgcaccgc gtcggcgtct tcgtcggcac
     2521 cagcacgtcc ggcgtgctcg agaccgagct cgcctaccgc cgccgcgatc ccgcaaccgg
     2581 ggcgttgccc acggactacc gctaccgcga gacccagaac acgggctcgg tgggagattt
     2641 cgcgcgccgc tacctcggtc tgcgcgggcc cgaagtcgtg atctcgtccg cgtgctcgtc
     2701 gtcggccaag gccttcggca acgccgcgcg catgatcgct gcgggcgtct gcgacgcggc
     2761 gatcgtcggc ggcgtcgact cgctgtgcct cacgacgctt tacggattcc attccctcga
     2821 gctgacgtcg cgcgcgccgt gccggccgtt cgacgccgag cgcgacggca tatcgatcgg
     2881 cgaaggcgcc ggattcgcga tcgtcgaaaa gccggaagcg cgcccggcgc gctcgcacgc
     2941 gctgctgctc ggcgtcggcg agagctcgga cgcctaccat atgtcgacgc cgcatcccga
     3001 gggcctgggc gcgcgcacgg cgatggagcg cgcgctcgcg agcgccgggc tcgcggcgtc
     3061 cgacgtcgac tacatcaacc tgcacgggac ggcgacgcgc acgaacgacg cgtccgagga
     3121 ccgcgcggtc cgcgatctgt tcggcgtggg cacgccgtgc agctcgacca agggagcgac
     3181 cggccacctg ctcggcgccg cggggatcac cgaggcgatc gtctccattc tcgcgatcca
     3241 gggcggattc atgccgggta gcccgggcac ccgaaggatc gatccctccc tcgggagcgg
     3301 ctatctcatc gagacgcgcc ccgcgaagct gcggcgcgtc ctctcgaatt ccttcggctt
     3361 cggcggcagc aactgcagcc tcgtgctcgg agcgccctga gccatggcga tgcgcgccta
     3421 cgtcgaaggc atcgcggtcc tcggccccgg gttagcgggc tgggaggcga gccgagcggt
     3481 gctggccggg accgcgccgt atcgccccga ggcggccgtc gtgccgccga gcgacctgct
     3541 gccgcccgcc gagcgccgcc gcacgggcct gccggtcaag ctcgcgctcg ccgtcggccg
     3601 cgaggcgttc ctcaacgccg gccgcgacgc ctccgcgacg cgcacggtat tcacggcgtc
     3661 gtgcggcgac ggccagaacc tgcacgacat gtgcgagtcg ctcgcgcgcc ccgagcgcga
     3721 ggtctcgccg acgcgcttcc acaactcggt gcacaacgcc gccgcgggct actgggggat
     3781 cgcgacccgc tcgcgcgagg cgtccaccag cctgtgcgcg tacgacgcga gcttcggcgc
     3841 cgggctgctc gaggcgatgg cgcaggtcgc ggtcgacggc gtcgcggtcg cgctgctcgc
     3901 ctacgaccag ccgtatcccg agccgctgca ccgtgcgcgc ccgatcggcg gcgtgttcgc
     3961 ggccgcgctg gt
//
LOCUS       DATDMW010000315         7255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_221725, whole genome shotgun sequence.
ACCESSION   DATDMW010000315 DATDMW010000000
VERSION     DATDMW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221725"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VI319_13240"
     CDS             <1..399
                     /locus_tag="VI319_13240"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY6864855.1"
                     /translation="YPGMPEVGNMPLPKKLLRRGVTDMVRVSDARMSGTAYGTVVLHV
                     SPEAAAGGTLALVKSGDMIELDVPARRLQLAVSDEELARRRAAWRAPQPPMERGYTRL
                     YVDHVLQADRGADLDFLVGASGAAVPRDNH"
     gene            complement(396..878)
                     /locus_tag="VI319_13245"
     CDS             complement(396..878)
                     /locus_tag="VI319_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY6864856.1"
                     /translation="MSTPSKNGRRIVVRRSPIHGRGVFALARIPKGARIIEYEGERID
                     DAQALERYPERMDGENHTFIFEVAEDLNIDGGSGGNSSRFINHSCDPNCETVDEDCRI
                     FVEAIRDIEPGEELTYDYNIDAGEPITPAVKRRWPCRCGGAGCRGTVLVPTEEASEAA
                     "
     gene            complement(922..2775)
                     /gene="typA"
                     /locus_tag="VI319_13250"
     CDS             complement(922..2775)
                     /gene="typA"
                     /locus_tag="VI319_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY6864857.1"
                     /translation="MTRSLRNIAIIAHVDHGKTTLVDKLLHQSGTFAAHQHVAERVMD
                     SNDIERERGITILAKNTAIDYQGVHINIVDTPGHADFGGEVERSLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALALGLRPILVVNKIDRPGARPDFVVNATFDMMDRLGASEEQLDF
                     PVVYASALQGTATLDARQPGTDMRPLFETILERVPAPKGDPDGPLQLQISNLDYSSYV
                     GRIGIGRIARGRLKPAQDVVVLEGTGAAPGARSRVNQVMGFRGLERLPMEEAAAGDIV
                     LVTGVEGVGIGVTLADPEHPEALPMLRVDEPTLTMNFQVNTSPFAGQEGRFVTSRQLR
                     ERLLRELQGNVALRVEETSDADVFVVSGRGELHLTILLENMRREGYELAVSRPRVVLK
                     EVDGVACEPFEALSVDVEAEHQGPVMESLGARRGELQDMQPDGRGRVRLDYRVPARGL
                     IGFQGEFMNLTRGTGLMSHVFDDYGPMKPAIASRRNGVLVSAEQGEAVAYALWKLQER
                     GRLMVGPGERVYEGMVIGLHSRDNDLVVNPIREKHLTNIRAAGKDEAILLTPPIRLTL
                     ELAVEFIEDDELVEVTPASIRIRKRHLAEHERRRAARAADPERARSAEAGS"
     gene            2886..3626
                     /locus_tag="VI319_13255"
     CDS             2886..3626
                     /locus_tag="VI319_13255"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY6864858.1"
                     /translation="MSPSVPAAPRFYVPFQLASGAEVELPERAARHVAVLRLARGDAI
                     TLFDGSGGQFAAEVTRVSRDSAHARIGRKDPIERESPLSVTLAQCVSAGDRMDLAVQK
                     ATELGVRRIVPLASERSIVRLSSERADRRLAHWRGIVTAACEQSGRNSVPEVLPITDL
                     DAFLAQADAGELRLVPSPDADRRLKAFPPARAVVLLVGPEGGLSAAERERARAKGYAA
                     VRFGPRVLRTETAPLAVIAAMQVLWGDC"
     gene            3667..4983
                     /gene="argA"
                     /locus_tag="VI319_13260"
     CDS             3667..4983
                     /gene="argA"
                     /locus_tag="VI319_13260"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEY6864859.1"
                     /translation="MPAHPDAQFISWFRSVAPYFHAFRGRTFVIAFGGEVLSEGRFVD
                     LAHDLNLLHSAGIRLVLVHGSRPQIEAQLRRRRARSRYANGLRVTDPVALECVKEAVG
                     LLRVEIETLLSLGLPNSPMAGAVINTASGNFITAQPMGVHDGVDFQYTGSVRKVDAQA
                     IAGCLDAGNIVIVSPVGYSPSGEVFNVAMEDVAVATAKALRAEKLIFFSDTVVEDERG
                     ETVSELTAQEAAERLARENAMSDDTRLYLAHAVEGVRGGVGRAHVISRNVNGALLIEL
                     FTHDGSGTMITADKVVKLRPAGVDDIGGILRLLEPLEADGTLVRRGRELFEREIDRFA
                     VIEHDGVIVGCAALYPFPKAAAGELACLAVREDYRDAGYGERLVRYIESQARAARMKK
                     LFVLTTRATHWFIERGFSTARVTSLPRERKALYNYQRRSKILVKPL"
     gene            5031..5303
                     /locus_tag="VI319_13265"
     CDS             5031..5303
                     /locus_tag="VI319_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEY6864860.1"
                     /translation="MARTVKCIKLGREAEGLDFPPYPGELGKRLYENVSKEAWQAWIK
                     HQTMLINENRLNLADPRARKYLAEQLEKHFFGGGADVASGYVPPES"
     gene            complement(5304..5966)
                     /gene="rpiA"
                     /locus_tag="VI319_13270"
     CDS             complement(5304..5966)
                     /gene="rpiA"
                     /locus_tag="VI319_13270"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY6864861.1"
                     /translation="MTQDELKQAVAREAMRHVVDGAVIGVGTGSTANFFIDELGRAKQ
                     RVAGAVASSERTAERLRSHGIRVLELNEVDELPVYVDGADEVNRGLAMIKGGGGALTR
                     EKIVAEVARKFVCIADESKLVDTLGRFPLPIEVIPMARRHVERRLSALGGRPKLREGF
                     TTDNGNVILDVHGLAIADPRATEAAIDGIVGVVTNGLFARRGADVLLLGAPGGVKTLI
                     AV"
     gene            complement(5974..>7255)
                     /gene="lpdA"
                     /locus_tag="VI319_13275"
     CDS             complement(5974..>7255)
                     /gene="lpdA"
                     /locus_tag="VI319_13275"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY6864862.1"
                     /translation="NVGCIPSKALLHTAAIMDAAEALAEHGVEFGKPKVDLDKLRAFK
                     TKVVGKLTGGLAAMAKMRKVTVIQGAGTFVGPNHLAVETATGKKTVGFASAIIAAGSQ
                     SAKLPFLPDDPRIVDSTGALELRASPKRMLVIGGGIIGLEMGTVYSTLGARLDVVEML
                     DGLMLGADRDLVKVWQSLNARRFDKVMLRTKTTKVEARKEGLKVWFEGENAPKEPQLY
                     DMILLSVGRTPNGKKIGAEAAGVSVDARGFIAVDSQMRTNVPHIYAIGDIARQPMLAH
                     KAVHEGHVAAEAAAGQKSHFDATVIPSVAYTDPEIAWAGVTEDEAKKQGLGYGVAKFP
                     WSASGRAIANGRDEGFTKLIFSEESHRIIGAGIVGTDAGNLISELALAIEMGADAVDI
                     GRTIHPHPTLGESVGMAAEMYEGVCTDVPPIRRK"
BASE COUNT         1043 a         2544 c         2523 g         1145 t
ORIGIN      
        1 tacccgggca tgcccgaggt cggcaacatg ccgctgccga aaaaactcct gcgccgcggc
       61 gtcaccgaca tggtgcgcgt gtccgacgcg cgcatgagcg ggacggccta cggcacggtg
      121 gtgctgcacg tgtcgcccga ggcggccgcc ggggggacgc tggcgctggt gaagagtggc
      181 gacatgatcg agctcgacgt gcccgcgcgc cgattgcagc tcgcggtctc cgacgaggag
      241 ctcgcacgcc ggcgcgcggc atggcgggcg ccgcagccgc cgatggagcg cggctacacc
      301 cggctctacg tcgatcatgt gctccaggcc gaccgcggcg cggatctcga cttcctggtc
      361 ggggcgagcg gggccgcggt cccgcgcgac aaccactagg cggcttcgga ggcttcttcc
      421 gtaggcacga gcaccgtgcc gcggcagccg gctccgccgc agcggcacgg ccagcgccgc
      481 ttcaccgccg gcgtgatcgg ctctcccgcg tcgatgttgt agtcgtaggt gagctcctcg
      541 ccgggctcga tgtcgcggat cgcctcgacg aagatgcggc agtcctcgtc caccgtctcg
      601 cagttcggat cgcaggagtg gttgatgaag cgcgagctgt tgccgccgct accgccgtcg
      661 atgttgaggt cctcggcgac ctcgaagatg aaggtatggt tctcgccgtc catgcgctcc
      721 gggtaccgct cgagcgcttg ggcgtcgtcg atccgctcgc cctcgtactc gatgatgcgc
      781 gcgcccttgg ggatgcgcgc gagcgcgaac accccgcgcc cgtggatcgg cgagcggcgc
      841 acgacgatgc gccggccgtt cttggatggt gtgctcatga agtcctgaga tgcggaaacg
      901 gacggtccgt tcaacgccgg tctagctccc cgcctcggcg gatcgtgcgc gctcggggtc
      961 cgccgcacgc gccgcgcgcc ggcgctcgtg ctcggccaga tggcgcttgc ggatccggat
     1021 cgacgccggc gtcacctcga ccagctcgtc gtcctcgatg aactcgacgg cgagctcgag
     1081 cgtcagccgg atgggcggcg tgagcaggat cgcctcgtcc ttgccggcgg cgcggatgtt
     1141 ggtgaggtgc ttctcgcgga tcgggttcac gacgaggtcg ttgtcgcggc tgtgcaggcc
     1201 gatcaccatg ccttcgtaga cgcgctcgcc gggaccgacc atcaggcggc cgcgctcctg
     1261 cagcttccac agcgcgtagg ccacggcctc gccctgctcg gcggacacca gcacgccgtt
     1321 gcggcgcgag gcgatcgccg gcttcatcgg gccgtagtcg tcgaacacgt ggctcatgag
     1381 gccggtgccg cgcgtgaggt tcatgaactc gccctggaaa ccgatcagtc cgcgcgcggg
     1441 aacccggtag tcgagccgca cgcggccgcg gccgtcgggc tgcatgtcct gcagctcgcc
     1501 gcggcgcgcg ccgagcgact ccatgaccgg gccctggtgc tcggcctcga cgtcgaccga
     1561 gagcgcctcg aagggctcgc acgcgacgcc gtcgacctcc ttgagcacga cgcgcggccg
     1621 cgacaccgcc agctcgtatc cctcgcggcg catgttctcg aggaggatgg tgaggtgcag
     1681 ctcgccgcgc cccgacacga cgaagacgtc ggcgtccgag gtctcctcga cgcgcagcgc
     1741 gacgttgccc tgcagctcgc gcagcagccg ctcgcgaagc tgacggctgg tgacgaatcg
     1801 gccttcctgc ccggcgaacg gcgaggtgtt gacctggaag ttcatggtga gggtcggctc
     1861 gtcgacgcgc agcatcggca gcgcctcggg gtgctcgggg tcggcgagcg taacgccgat
     1921 gccgacgccc tcgacgcccg tgacgaggac gatgtcgccc gccgcggctt cctccatcgg
     1981 cagccgctcg aggccgcgga aacccatcac ctggttgacc cgcgagcggg cgccgggcgc
     2041 ggcgccggtc ccctcgagga ccacgacgtc ctgcgcgggc ttcaggcgtc cgcgcgcgat
     2101 ccggccgatg ccgatccgcc cgacgtagct cgagtagtcg aggttgctga tctgcagttg
     2161 cagcgggccg tcagggtcgc ccttcggcgc ggggacgcgc tcgaggatcg tctcgaagag
     2221 cggcctcatg tccgtgcccg gctgcctggc atcgagcgtg gcggtgccct ggagcgcgga
     2281 ggcgtatacg accgggaagt cgagctgctc ctccgaggca cccagccggt ccatcatgtc
     2341 gaacgtcgcg ttcaccacga agtccggccg cgcgccgggg cggtcgatct tgttcacgac
     2401 caggatcggc ctcaggccga gcgcgagcgc cttgcgcgtc acgaagcgcg tctgcggcat
     2461 cgggccctcc accgcgtcga cgagcagcag cacgccgtcg accatggaga gcgagcgttc
     2521 cacctcgccg ccgaagtcgg cgtgcccggg ggtgtccacg atgttgatgt gcacgccctg
     2581 gtagtcgatc gccgtgttct tggcgaggat ggtgatgccg cgctcgcgct cgatgtcgtt
     2641 cgagtccatg acgcgctcgg cgacgtgctg gtgcgcggcg aaggtccccg actggtgcag
     2701 cagcttgtcg acgagcgtcg tcttgccgtg gtcgacgtgg gcgatgatgg cgatgttacg
     2761 gagggagcgt gtcacgcgaa ggcgcagtcg aagattcggg gcaaagccga gcattttatc
     2821 agttggaccc ggcccggcga agcctttctt gcgaagtccg gcgtcgaggc cgcaaaatcc
     2881 attgcatgag cccatcggtc ccggccgcgc cgcggttcta tgtgcctttc cagctcgcct
     2941 cgggggcgga ggtcgagctt cccgagcggg ccgcgcggca cgtcgcggtc ctgcgcctcg
     3001 cgcgaggcga cgcgatcacc ctgttcgacg gctcgggcgg gcagttcgcc gcggaggtga
     3061 cgcgcgtctc gcgcgacagc gcgcacgcgc gaatcggccg caaggacccg atcgagcgcg
     3121 aatccccgct ttcggtgacg ctcgcccagt gcgtctcggc cggcgaccgc atggacctcg
     3181 cggtgcagaa ggcgaccgag ctcggcgtgc gccgcatcgt gccgctcgcg agcgagcgct
     3241 ccatcgtgcg gctttcctcc gagcgcgccg accggcgcct cgcgcactgg cgcggcatcg
     3301 tcacggccgc gtgcgagcag tccgggcgca acagcgtccc ggaggtgctg ccgatcaccg
     3361 acctggacgc tttcctggcc caggcggacg cgggcgagct gcggctggtg ccttcgcccg
     3421 acgcggaccg gcgcctgaag gcgtttccgc cggcgcgcgc cgtggtcctg ctcgtcggtc
     3481 ccgagggcgg gctttcggcc gccgagcgcg agcgcgcccg ggcgaagggc tacgcagcgg
     3541 tgcgcttcgg cccgcgcgtg ctgcgcaccg agaccgcgcc gctcgcggtg atcgcggcaa
     3601 tgcaggttct ctggggagat tgctagaatc gcgccaggga agacacagcg gcgaacaacg
     3661 aaaaccatgc cggcacaccc ggacgcacaa ttcatcagct ggttccgctc ggtcgcgccc
     3721 tacttccacg cgttccgcgg caggacattc gtgatcgcct tcggcggcga ggtgctgtcc
     3781 gagggcaggt tcgtcgatct cgcgcacgac ctcaacctgc ttcacagcgc gggcatacgg
     3841 ctcgtcctcg tccacggatc gcggccgcag atcgaggcgc agctgcggcg ccgccgcgcg
     3901 cgctcgcgct acgccaacgg cctgcgcgtc accgacccgg tcgcgctcga atgcgtgaag
     3961 gaggcggtgg gcttgctgcg cgtcgagatc gagaccctgc tctcgctcgg gctgccgaac
     4021 tcgcccatgg cgggcgccgt catcaacacg gcctcgggca atttcatcac ggcgcagccg
     4081 atgggcgtgc acgacggcgt cgacttccag tacaccggat cggtgcgcaa ggtcgacgcg
     4141 caggcgatcg cgggctgcct cgacgcgggc aacatcgtca tcgtctcgcc ggtcggctac
     4201 tcgccgagcg gcgaggtgtt caacgtcgcc atggaggacg tggccgtggc aaccgcgaag
     4261 gcgctgcgcg ccgagaagct catcttcttc tccgacacgg tggtggagga cgaacgcggc
     4321 gagaccgtct cggagctgac cgcgcaggag gccgccgagc gcctcgcgcg cgagaacgcg
     4381 atgagcgacg atacgcggct gtacctcgcg cacgcggtcg agggcgtgcg cggcggcgtc
     4441 gggcgcgcgc acgtcatcag ccgcaacgtc aacggcgcgc tgctcatcga gctcttcacg
     4501 cacgacggct cgggcacgat gatcaccgcc gacaaggtgg tgaagctgcg tcccgccggg
     4561 gtcgacgaca tcggcggcat cctgcggctg ctcgagccgc tcgaggccga cggcacgctg
     4621 gtgcgccgcg gccgcgagct gttcgagcgc gagatcgacc gcttcgccgt gatcgagcac
     4681 gacggcgtca tcgtcggctg cgccgcgctc tacccgttcc cgaaggcggc ggcgggcgag
     4741 cttgcctgcc tcgcggtccg cgaggactac cgcgacgccg gctacggcga gcgcctggtg
     4801 cgctacatcg agagccaggc gcgcgcggcc cggatgaaga agctgttcgt gctcaccaca
     4861 cgcgcgacgc attggttcat cgagcgcggg ttctcgaccg cgcgggtcac atcgctgccc
     4921 cgggagagga aggcgctgta caactaccag cgtcgctcca agatcctggt gaaaccgctc
     4981 tgagggaacc ggcgggctac aatcgtctca aaggcaaacg gagtcccgac atggccagaa
     5041 cggtgaaatg catcaagctc gggcgcgagg cggagggtct cgacttcccg ccttacccgg
     5101 gcgagctcgg caagcgcctt tacgagaacg tctccaagga ggcgtggcag gcctggatca
     5161 agcaccagac catgctgatc aacgagaacc ggctgaacct cgccgatccg cgtgcccgca
     5221 aatacctcgc cgagcagctg gagaagcatt tcttcggcgg gggcgctgac gtcgcgtccg
     5281 gctacgtccc gcccgagagc tgatcagacc gcgatcagcg tcttcacccc gcccggcgcc
     5341 ccgagcagca gcacgtcggc gccgcgccgg gcgaacaggc cgttggtgac cacgcccacg
     5401 atgccgtcga tcgcggcctc ggttgcccgc ggatcggcga tcgcgagccc gtgcacatcg
     5461 aggatgacgt tgccgttgtc ggtggtgaag ccttcgcgca gcttcggccg cccgccgagc
     5521 gccgagagtc tgcgctcgac gtggcggcgc gccatcggaa tgacctcgat cgggagcggg
     5581 aaccgtccca gcgtgtcgac gagcttggac tcgtcggcga tgcagacgaa cttgcgcgca
     5641 acctcggcga cgatcttctc gcgcgtgagc gcaccgccgc cgcccttgat catggcgagg
     5701 ccgcggttca cctcgtcggc accgtcgacg tagaccggca gctcgtcgac ctcgttcagc
     5761 tcgagcacgc gaatcccgtg cgatctgagc cgctcggcgg tgcgctcgga gctcgcgacc
     5821 gcgcccgcga cccgctgctt cgcgcgcccc agctcgtcga tgaagaagtt cgccgtcgag
     5881 ccggtgccga cgccgatgac cgcaccgtcg accacgtgcc gcatcgcctc gcgcgcgacg
     5941 gcctgcttca gctcgtcctg ggtcattccg gcgtcattta cggcgaatcg gcggcacgtc
     6001 ggtgcacacg ccctcgtaca tctccgccgc catgccgacc gactcgccga gcgtcgggtg
     6061 cgggtggatg gtcctgccga tgtcgaccgc gtcggcgccc atctcgatcg cgagcgcgag
     6121 ctcgctgatc aggttgccgg cgtcggtacc gacgatgccc gcgccgatga tgcggtggct
     6181 ctcctcggag aagatcagct tggtgaagcc ctcgtcacgg ccattcgcga tcgcgcggcc
     6241 cgaggcgctc caggggaact tggcgacgcc gtagccgagg ccctgcttct tcgcctcgtc
     6301 ctcggtgacg ccggcccagg cgatctccgg gtccgtatac gcgaccgacg ggatcaccgt
     6361 cgcgtcgaaa tgcgacttct gtccggcggc ggcctcggcc gcgacatggc cttcgtgcac
     6421 cgccttgtgc gcgagcatcg gctgccgcgc gatgtcgccg atggcgtaga tgtgcggcac
     6481 gttggtgcgc atctgcgagt cgaccgcgat gaagccgcgc gcgtcgacgc tcaccccggc
     6541 cgcctcggcg ccgatcttct tgccgttcgg cgtccggccg accgagagca ggatcatgtc
     6601 gtagagctgc ggctccttgg gcgcattctc gccttcgaac cagaccttca ggccctcctt
     6661 gcgcgcctcg accttggtgg tcttcgtcct gagcatgacc ttgtcgaagc ggcgcgcgtt
     6721 gagcgactgc cacaccttca cgaggtcgcg gtcggcgccg agcatcagac cgtcgagcat
     6781 ctcgaccaca tcgagacgcg cgccgagcgt cgaatacacg gtccccatct ccaggccgat
     6841 gatgccgccg ccgatcacga gcatgcgctt ggggctcgcg cgcagctcga gcgcaccggt
     6901 cgagtcgacg atgcgcggat cgtccggcaa gaacggcagc ttggccgact gcgatcccgc
     6961 tgcgatgatc gcactcgcga acccgaccgt cttcttcccg gtcgcggtct cgaccgcgag
     7021 atggttcggg ccgacgaacg tgccggcgcc ctggatcacc gtgaccttgc gcatcttcgc
     7081 catcgcggcg aggccgccgg taagcttgcc gacgaccttg gtcttgaacg cgcgcagctt
     7141 gtccaaatcc actttgggtt ttccgaactc cacgccgtgc tcggcgagcg cctccgccgc
     7201 gtccatgatc gccgcggtgt gcagcagcgc cttggaggga atgcagccca cgttg
//
LOCUS       DATDMW010000316         2430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1713996, whole genome shotgun sequence.
ACCESSION   DATDMW010000316 DATDMW010000000
VERSION     DATDMW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713996"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..499
                     /locus_tag="VI319_13280"
     CDS             161..499
                     /locus_tag="VI319_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864863.1"
                     /translation="MMRPRVVFAVVFTVVFAAVYLVAVWKNYALFTYDAAAGRFAPGV
                     QRLAEGEGVAMYWYGWMATAGLAALASGLAASFVPERLAQKLWSGWSWLVPLGVVLVF
                     SYLLRNYFLR"
     gene            509..892
                     /locus_tag="VI319_13285"
     CDS             509..892
                     /locus_tag="VI319_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY6864864.1"
                     /translation="MWRLPIALACLVLGASSAWAGGENADLMDEHSEDEGPPYFGFVR
                     DVRGNPLKDAKVSLKFAKANITYVIQTNVLGMYRMRNPNKDWKPEDLTISCAKEGYRQ
                     TRALRRTANVGGNAAIETECQMQKE"
     gene            889..1884
                     /locus_tag="VI319_13290"
     CDS             889..1884
                     /locus_tag="VI319_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864865.1"
                     /translation="MTRSAGVRCIVFLALLAPACAFAEQDIVLRYGMAISAQKSIYSL
                     PIAVAEREGLFRREGLDFRLVIPLPGGSDKMIDALHDDTVDVTHVATPFLVRAALAGS
                     DAVAIAAEFNNPIYSLVARPDITKIGELKGQVVGLADPGGSITLSIRKLLELRGLREG
                     DIQVRTIEGTPARLSCLQRGECAAVPLGQPQDLVAASEGYRVLGLSTEAVPQFVYTVT
                     AVRRSWAAAHPDAVVRYVRALGAAFAVIRDPTRRDRVVEIIVETTGVTPEIARGTLAL
                     YFEPERNVVPMRGEIDLEGFARVIDFMAEAGQLKGPLPAAGRFVETRYLREAGVR"
     gene            complement(1881..>2430)
                     /locus_tag="VI319_13295"
     CDS             complement(1881..>2430)
                     /locus_tag="VI319_13295"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6864866.1"
                     /translation="AEQQLVSANAQIGVAKAAMFPTITLTGSTGVESNALSSLHDSGS
                     RIWSLGFGLSLPLFDAGRYRARTDAATARERQAVAGYQKAVETAFREVSDALASVQST
                     SGAEKDYQIQLEAARRALRLARLRYEAGYSAFLEVLDAQRTANDAELAYLRNRQALLS
                     ATVDLMKALGGGWSPQQVSAAR"
BASE COUNT          351 a          821 c          876 g          382 t
ORIGIN      
        1 tcgggctcgc ggcgctgccc gcgtactggg ccttctggcg ccagccgctc gacgaagcgc
       61 acgcgcgcac gcgcgcgatc ctcacggcgc tgctcgcgtt catcgtctgg tggaatttcc
      121 tggtcgggca catcctgaac aacatccggg gattcggatc atgatgaggc cccgcgtcgt
      181 gttcgcggtc gtgttcaccg tggtgttcgc cgcggtgtat ctcgtcgccg tctggaagaa
      241 ctacgcgctg ttcacctatg acgcggcggc cggacggttc gcgccggggg tgcagcgcct
      301 cgcggaaggg gaaggcgtcg ccatgtactg gtacggatgg atggccacgg cgggcctcgc
      361 ggctctcgcc tcgggtctcg cggcatcctt cgtccccgag cgcctcgcgc agaagctgtg
      421 gtccggctgg tcctggctcg tgccgctcgg cgtcgtgctg gtattctcgt accttctgag
      481 aaactatttc ctccgctagg aggcgaacat gtggagactt ccgatcgcgc tcgcgtgcct
      541 cgtgctcggc gcgtcctcgg catgggctgg aggagagaac gccgacctga tggacgagca
      601 ttccgaggac gaaggcccgc cttacttcgg cttcgtccgc gacgtccgcg gcaatccgct
      661 caaggacgcg aaggtgagcc tcaagttcgc gaaggcgaac atcacctacg tgatccagac
      721 caacgtcctc gggatgtacc ggatgcgcaa tccgaacaag gactggaaac ccgaggacct
      781 cacgatctcg tgcgccaagg aaggctaccg ccagacgcgg gcgctgcgtc gcacggcgaa
      841 cgtcggcggc aacgcggcga tcgagaccga atgccagatg cagaaggagt gacccgtagc
      901 gcgggcgtcc gctgcatcgt attcctggcg ctgctcgcgc cggcctgcgc cttcgcggag
      961 caggacatcg tgctgcgcta cgggatggcg atctcggcgc agaagagcat ctattcgctg
     1021 cccatcgcgg tcgccgagcg cgagggcctg ttccgccgcg agggcctcga cttccgcctc
     1081 gtgatcccgc tcccgggcgg ctcggacaag atgatcgatg cgctccacga cgacaccgtc
     1141 gacgtgacgc acgtcgcgac gccgttcctg gtgcgcgccg cgctcgcggg ctcggacgcg
     1201 gtcgcgatcg cggccgagtt caacaacccg atctattccc tcgtggcccg gcccgacatc
     1261 acgaagatcg gcgagctgaa ggggcaggtg gtgggcctgg ccgatccggg cggaagcatc
     1321 accctgtcga tccgcaagct gctcgagctg cgcggccttc gcgaggggga catccaggtg
     1381 cggaccatcg aaggcacgcc ggcccggctg agctgcctcc agcgcggcga atgcgccgcg
     1441 gtgccgctcg gccagccgca ggacctcgtg gccgcgagcg aaggctaccg ggtcctcggg
     1501 ctctccaccg aagccgtgcc gcaattcgtc tacaccgtga cggctgttcg ccgctcgtgg
     1561 gccgcggcgc atcccgacgc cgtggtgcgc tacgtgcgcg cgctcggagc ggcgttcgcc
     1621 gtgattcgcg acccgacgcg gcgcgaccgg gtggtggaga tcatcgtcga gacgaccggg
     1681 gtgacgccgg agatcgcccg aggcacgctg gcgctctact tcgagcccga gcgcaacgtc
     1741 gtgccgatgc ggggggagat cgacctcgag ggtttcgcgc gggtcatcga cttcatggcc
     1801 gaggcggggc agctcaaggg cccgctcccc gcggcggggc gcttcgtcga gacgcgctac
     1861 ctgcgcgagg ccggagtgcg ctagcgcgcg gcgctgacct gctgcggcga ccagccgccg
     1921 ccgagcgcct tcatgaggtc caccgtcgcc gagagcagcg cctggcggtt gcgcaggtac
     1981 gcgagctcgg cgtcgttggc ggtgcgctgc gcgtcgagca cctcgaggaa ggccgagtag
     2041 ccggcctcgt agcgcaggcg cgcgaggcgc agcgcgcggc gcgcggcctc gagctgaatc
     2101 tggtagtcct tctcggcgcc ggaagtggac tgcacgctcg cgagcgcgtc ggagacctcg
     2161 cggaacgcgg tctcgacggc cttctggtag ccggcgaccg cctggcgctc gcgcgcggtg
     2221 gcggcgtcgg tgcgggcgcg gtagcggccc gcgtcgaaga gcggcagcga cagcccgaag
     2281 ccgagcgacc agatgcggct gcccgagtcg tgcagcgagg acagcgcgtt gctctcgacg
     2341 ccggtcgagc ccgtgagcgt aatcgtcggg aacatggcgg ccttggcgac gccgatctgc
     2401 gcgttcgcgg agacgagctg ctgctcggcc
//
LOCUS       DATDMW010000317        14279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1962116, whole genome shotgun sequence.
ACCESSION   DATDMW010000317 DATDMW010000000
VERSION     DATDMW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14279
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962116"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /gene="glyS"
                     /locus_tag="VI319_13300"
     CDS             complement(<1..1150)
                     /gene="glyS"
                     /locus_tag="VI319_13300"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY6864867.1"
                     /translation="MAKPKPAAASAPLLIELLTEELPPKALAGLGRVFADEIFNGLER
                     HHLAQRDATGCKVFATPRRLAVLIPGVRAIAPDRSSEVDGPSLKAPPQAVAGFARKHG
                     VTVSDLGQRETPKGAVYVARVHATGAKLDAVLADVLNEALKKLPIPKTMRWGSGDAQF
                     VRPVHGLLLVHGAKALKLAEPVLGFAGGTTRTRGHRFLAKAEVSVGNAADYEAILERD
                     GCVVASFEQRRERIHKALEKKAGGAKILSDDALLDEVTALVEWPEVLQGAFDEEFLVV
                     PQECLVLSMRQHQRYFPLADARGTLAPRFLVVSNMPPSRSGAIVRGNERVLRARLSDA
                     KFFYDQDRRERLERRVPGLATVVFHNKLGSQLERVERIQLLTGRIAHDL"
     gene            complement(1154..2056)
                     /gene="glyQ"
                     /locus_tag="VI319_13305"
     CDS             complement(1154..2056)
                     /gene="glyQ"
                     /locus_tag="VI319_13305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015422011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY6864868.1"
                     /translation="MLTFQELILKLQGYWDKQGCVLMQPYDMEMGAGTFHTASFLRAI
                     GPEPWKAAYVQPSRRPKDGRYGDNPNRLQHYYQYQVVLKPSPDDIQDLYISSLRALGI
                     DTREHDIRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQQVGGLDCKPVLGEITY
                     GLERLAMYLQGKDSIFDLIWTPGVSYGDVYHQNEVEQSRYNFELSDPAWLLEQFADYE
                     REAKRLIEAGAPLPGYEMVLKCSHTFNLLDARGAISVTERAAYIGRVRALARLVAKAY
                     YDSREKLGFPMLKQQKPEKKKTPA"
     gene            complement(2082..3593)
                     /gene="lnt"
                     /locus_tag="VI319_13310"
     CDS             complement(2082..3593)
                     /gene="lnt"
                     /locus_tag="VI319_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY6864869.1"
                     /translation="MRLGQLPPRAAVAVAFGSGAATVAGFAPAGIFPLPVLSLALLLG
                     LCRGATPRGALALGFAFGAGLFGVGASWVYVSLHDFGTMPVPLAGVATLGFCAILSLY
                     PAAAAWCLARLRARPATAALLAFPALWTLAEWLRGWVFTGFPWLAIGYSQTDSPLAGL
                     APVLGVYGVSWATALVAGLAFLVVAGEPRARIAGACGIAVVIAAGYGLGSASWTAPEG
                     APLRVSLLQGNIAQDLKFQEQRYAATLETYRRLVEASDATLIVLPETAVPRFFDTVDP
                     AYWNQLARLARDKHADLLVGVPVRDAEGRYFNSLISLGVSPPQIYSKSHLVPFGEFIP
                     PGFRWIVDTVAIPLADFSRGPERPRPLAVAGQRVAPDICYEDAFGEEIVRQLPEATLL
                     VNASNVAWFGDSLAPPQHLQMSRMRALETGRYMLRATNTGVTAVIDERGRVRSRLPQF
                     TEGALQASAQGFSGATPYVRWANAPALVLCALSLAAAAWLASRRRVGPTWESR"
     gene            complement(3590..4426)
                     /locus_tag="VI319_13315"
     CDS             complement(3590..4426)
                     /locus_tag="VI319_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY6864870.1"
                     /translation="MDDAQDHKPSLLERLSALLMREPEDREQLVNLLHSAYEHDLLDS
                     DALSMIEGVLQVSELQVRDIMVPRPQMDVIDANETPDKFIPHVIATAHSRFPVIDKDR
                     DDVIGILLAKDLLRYYAGEEEFKVRDMLRPAVFVPESKRLNVLLREFRANRNHMAIVV
                     DEYGGVAGLVTIEDVLEQIVGDIEDEYDFDESEDNIVRESSGHWRVKAQTEIGDFNRA
                     FGTDFSDEDFDTVGGLIIDRFGRLPKRGESVTIGGLRFQVLRADSRRLHALQVQRLPG
                     RA"
     gene            complement(4468..4914)
                     /gene="ybeY"
                     /locus_tag="VI319_13320"
     CDS             complement(4468..4914)
                     /gene="ybeY"
                     /locus_tag="VI319_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY6864871.1"
                     /translation="MPKAPALTLDVQLVARRSRFPSRAKVRRWARAAIMRPADVTVRV
                     VGEAEGRRLNRVYRGRDRATNVLSFAYDRGRAGVAGDLLLCAPVIRREALRQGKALEA
                     HYAHLVVHGLLHLQGYDHEAPRAARRMEALETQILAKLGYPDPYPG"
     gene            complement(4916..5866)
                     /locus_tag="VI319_13325"
     CDS             complement(4916..5866)
                     /locus_tag="VI319_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY6864872.1"
                     /translation="MTSAKIKPLEVILSPLDNQRLANLCGPLDENLRQIETALDVTLS
                     RRGERFTVRGAPGQAARAAEALQKFYGDAKRGLTVDDVQLGLVEFRTDPRSHETEDTP
                     LLTRRGDLRGRTPRQRQYIQNILEHDITFAIGPAGTGKTYLAVACAVDSLERDAVKRI
                     VLVRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLLGYDRVGKLFERGAIELAPL
                     AYMRGRTLNHSFIILDEAQNTTPEQMKMFLTRMGFGSKVVVTGDVTQIDLQRGQKSGL
                     VEVERILQDVRGIAFSTFKSDDVVRNPLVQRIVDAYEQKG"
     gene            complement(5890..7218)
                     /gene="miaB"
                     /locus_tag="VI319_13330"
     CDS             complement(5890..7218)
                     /gene="miaB"
                     /locus_tag="VI319_13330"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY6864873.1"
                     /translation="MAKKVYIRTFGCQMNEYDSAKMADVLEAAEGYEPAAAPDDADLI
                     LFNTCSVREKAQEKVFSDLGRIRHLKRDRPGVLIGVGGCVASQEGAAIVARAPFVDVV
                     FGPQTLHRLPALIEARRRTGRPQVDVSFPRIEKFDALPPPRVDGPTAFVSIMEGCSKY
                     CSFCVVPYTRGEEVSRPLEDVLTEIADLAEEGVREVTLLGQNVNAWRGTVDAEAADFA
                     LLLEYIAEIPGIDRIRYTTSHPREFTQRLVDAHAGIDELVPHVHLPVQSGSDRVLAAM
                     KRGYTVLEYKSIVRRLRAARPDVCITSDFIVGFPGETERDFEATLALVDELRFDSSFS
                     FLYSPRPGTPAAELADETPARVKLERLHALQARLDSHARAISEAMVGTRQNILVEGPS
                     RHDAGELAGRTANNRVVNMPGEANLVGRFVDATITAVLAHTLRGEIQAAR"
     gene            complement(7225..7551)
                     /locus_tag="VI319_13335"
     CDS             complement(7225..7551)
                     /locus_tag="VI319_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864874.1"
                     /translation="MAPSRALLLLSIALAVLPAAAQQRTIPADAKRGFLKHEREALVS
                     IDGAPARLAPGATIRDSHNLIIVPAAMPREGAWADYLLDRGGEVFRVWLLSDEERARP
                     RPAARR"
     gene            complement(7573..7649)
                     /locus_tag="VI319_13340"
     tRNA            complement(7573..7649)
                     /locus_tag="VI319_13340"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7613..7615),aa:Met,seq:cat)
     gene            7705..8892
                     /locus_tag="VI319_13345"
     CDS             7705..8892
                     /locus_tag="VI319_13345"
                     /inference="COORDINATES: protein motif:HMM:NF005788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HEY6864875.1"
                     /translation="MQVDVAIVGGGLVGASLAAALAGSGLEIALLDQSPPPEPAQGWD
                     ARIYALSPASVAFLRAAGAWQLLDPARSTPIHRMRVFGDDGVSELGFSAYEAGVAELA
                     VTVESGRLARALWSRLDGASGVRRLCPASPVALERSPEEIILRLASGEALRARLCVGA
                     DGARSWVRGAAGMTASTRPYGERGVVANFTCEVPHRNTAFQWFRGDGVLAWLPVADHV
                     VSIVWSTAETRARELLALSPDALCDAVAAAGARALGALTLVTPPASFPLERLHASRSA
                     RERVALLGDAAHVVHPLAGQGVNLGFADAAALAGLLRGMRGHRDAGDASLLRRFERKR
                     AEDILAMRWVTDGLHGLFGARHPAAARIRNLGLSLTDSIPLVKSLLARRAMGAADFSK
                     DAP"
     gene            8889..9587
                     /locus_tag="VI319_13350"
     CDS             8889..9587
                     /locus_tag="VI319_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY6864876.1"
                     /translation="MIIRSFAAVTLALAALSASADEASIRRAIEARFNGVAVESVTKT
                     PYSGLYEVVIGDEIVYTDDKVTYIFTGNIIDANTRRNLTEERQSQLLAVNFKDLPLDN
                     AIKTVRGSGKRVMVIFADPRCGFCKQFEQNLYKLTDVTIYTMLYPVIAPDSAEISRNI
                     WCSKNPSQAWLDTMLKGVLPPTNTCATPLDKNLALGKRLRVSGTPTTFLPNGQRIVGA
                     RFPELVQALDQASK"
     gene            complement(9593..10777)
                     /locus_tag="VI319_13355"
     CDS             complement(9593..10777)
                     /locus_tag="VI319_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEY6864877.1"
                     /translation="MIRPSRAAVLVAAALIAGCASAPSPVATDRCPCGRPAPPAPRVE
                     LYQEVAYPDIPGWTDGEQAAAWSALLASCQALRFRDAWRAACLDAASMPAPDDATARA
                     FIESRFAPWRLAAPDGRTEGLITGYYEPLLRGSRVRQPPFVVPLYAPPDDLLTIDLSA
                     VNPDLRNMRLRGRLQGRKVVPYWSRAEIEKGAAPVAGKELVWVDDAVEAFFLQIQGSG
                     RIRLDNGDVMRVGYADQNGHPYQSIGRTLVERGDLQPGEASMQAIHAWAESHPDRLPE
                     LLDQNPSYVFFRELPLTDPSVGPIGALGVPLTPQRSIAVDPRYVPLGAPAFLGTTWPS
                     SDLPLNRLVIAQDTGGAIRGPVRADYFWGFGSEAGALAGRMRQQGALWVLLPKGMKPP
                     AS"
     gene            10784..10927
                     /locus_tag="VI319_13360"
     CDS             10784..10927
                     /locus_tag="VI319_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864878.1"
                     /translation="MDSGLWIIVLEAGLALALLLFIVWWTVPKRRRDPESDAKSEERT
                     EKR"
     gene            complement(10943..11323)
                     /gene="apaG"
                     /locus_tag="VI319_13365"
     CDS             complement(10943..11323)
                     /gene="apaG"
                     /locus_tag="VI319_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY6864879.1"
                     /translation="MADKKYEVLVSSQTQFIPEQSDEQNSRFVFAYTMTIRNTGTVSA
                     QLVSRHWVITDARNQVQEVRGLGVVGAQPNLKPGESFEYTSGTAIATPVGTMRGSYQM
                     RAEDGTQFDADIPEFTLSVPRVLH"
     gene            11410..12096
                     /gene="rpe"
                     /locus_tag="VI319_13370"
     CDS             11410..12096
                     /gene="rpe"
                     /locus_tag="VI319_13370"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY6864880.1"
                     /translation="MAARVFRIAPSILSADFARLGDEVKTVVSAGADMIHFDVMDNHY
                     VPNLTVGPLVCRAIRPLTQADIDVHLMVKPVDRLVSDFAKAGANIISFHPEASEHVDR
                     TVGLIHDSGCKAGLVLNPATPLVVLDHVLHKLDLVLLMSVNPGFGGQEFIPQTLEKLR
                     AARRRIDEASQASGRAILLEVDGGIKVDNIAAVARAGADTFVAGSAIFGSGDYRATIA
                     KMREALQTAA"
     gene            12100..13500
                     /locus_tag="VI319_13375"
     CDS             12100..13500
                     /locus_tag="VI319_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6864881.1"
                     /translation="MAELLERLRREIGDAAVLAAPADWAPYAIDWRRRYSGRPLAVVK
                     PASTAEVAAVVRACAEARAAVVPQAGNTSLCGAATPDGSGTQVVLNVSRLNRVRSIDA
                     ANNAMTAEAGCVLASLHDAARGANRLFPLTLAAQGSCELGGNLSTNAGGTGVLRYGNA
                     RDLVLGLEVVLPSGEVWDGLRALRKDNTGYDLKQLFIGAEGTLGVITAAVVKLFPLPR
                     SQATAVVALPDAERSLALLGTAQSHCGERLTAFEIFSHACLELVLAHFPGTSAPFRSP
                     YPQYALLEVSDSGAGADAALEAALAEAMEKGSVLDAVVAQSESQARALWALRENISEA
                     QAREGPNIKHDVSVPVSSIPAFAAATGAELARAFPGSRLIVFGHLCDGNLHYNLSPPP
                     GVSAETFMGNLDAVNRVVHDSVARFRGSISAEHGIGRLKREELARYKTPLELDLMRAV
                     KRAIDPLGIMNPGKVF"
     gene            13698..>14279
                     /locus_tag="VI319_13380"
     CDS             13698..>14279
                     /locus_tag="VI319_13380"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880785.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY6864882.1"
                     /translation="MTEEKFKQLAAQGYNRVPVVAETFADLDTPLSIYLKLANRSNTY
                     LLESVVGGERFGRYSFIGLATPIRIRAVGRLVTVMHGDAVTERSQSGDPLQFVREFLS
                     RYKIAEPPGLPRFCGGLVGYFGYDTVRWIEPKLSGGWLKRDPLGCPDMLLLVSEELAV
                     VDNLTGKLYLVVFADPSKPRAWDDAQARLRELLG"
BASE COUNT         2144 a         4903 c         4998 g         2234 t
ORIGIN      
        1 cgaggtcgtg cgcgattcgg ccggtaagca gctgaatgcg ctcgacgcgc tcgagctggc
       61 ttccgagctt gttgtggaac acgaccgtcg cgagcccggg cacgcggcgc tccaggcgct
      121 cccggcggtc ctggtcgtag aagaacttgg cgtcggagag gcgcgcgcgc agcacgcgct
      181 cgttgccgcg cacgatcgcg cccgagcgcg acggcggcat gttgctcacg acgagaaagc
      241 gcggcgccag cgtgccgcgc gcgtcggcga gcgggaaata gcgctggtgc tggcgcatcg
      301 aaagcacgag gcactcctgg ggaacgacca gaaattcctc gtcgaaggct ccctgcagga
      361 cctccggcca ctcgaccagc gcggtgactt cgtccaggag cgcgtcgtcg gaaaggatct
      421 tcgcaccccc ggccttcttc tcgagcgcct tgtggatgcg ctcccggcgc tgctcgaagc
      481 tcgcgacgac gcagccgtcg cgctcgagaa tcgcctcgta gtcggccgcg ttgccgacgg
      541 agacttccgc cttggccagg aaacgatggc cgcgcgtgcg ggtcgttccg cccgcgaagc
      601 cgagcaccgg ctccgcgagc ttcagtgcct tggctccgtg cacgaggagc agcccgtgca
      661 ccggacgcac gaattgcgcg tcgcccgagc cccagcgcat ggtcttgggg atcggcagct
      721 tcttcagggc ttcgttcagc acgtcggcga ggaccgcgtc cagcttcgcg cccgtggcgt
      781 ggacacgggc gacgtatacg gcgcctttcg gcgtttcccg ctgcccgaga tcggaaacgg
      841 tcaccccgtg tttcctcgcg aatccggcga cggcttgggg cggtgccttc agcgacgggc
      901 cgtccacttc gctggagcgg tccggcgcga tcgcgcgtac tcccggaatc aacaccgcga
      961 gtcggcgcgg ggtggcgaag accttacatc ccgtcgcatc ccgctgcgcc aggtggtgcc
     1021 gctccaggcc gttgaagatc tcgtcggcga agacgcggcc caggcccgcg agcgccttgg
     1081 gtggcagctc ctcggtcagc aattcgatca gcaagggggc cgacgcggcc gccggcttcg
     1141 gtttagccat cgctcaggcc ggcgtctttt ttttctcggg cttctgctgc ttgagcatcg
     1201 ggaagccgag cttttcgcgc gaatcgtagt acgccttcgc cacgagacgg gcgagcgcgc
     1261 gcacgcgccc gatatacgcc gcgcgctccg tcaccgagat cgcgccgcgc gcatcgagca
     1321 agttgaacgt gtgcgagcac ttgagcacca tctcgtagcc cggcaagggc gccccggcct
     1381 cgatcagccg tttcgcctcg cgctcgtagt cggcgaactg ctcgagcagc cacgccgggt
     1441 cggacagctc gaagttgtat cgcgattgct cgacctcgtt ctggtggtag acatcgccgt
     1501 aggagacgcc cggtgtccag atcagatcga agatgctgtc cttgccctgc aggtacatcg
     1561 cgaggcgctc cagcccgtag gtgatctcgc cgagcaccgg cttgcagtcg aggccgccca
     1621 cctgctggaa ataggtgaac tgcgtgacct ccatcccgtc gagccagact tcccagccca
     1681 ggccccacgc gccgagcgtc ggcgactccc agtcgtcctc gacgaagcgg atgtcgtgct
     1741 cgcgcgtgtc gatgccgagc gcgcgcagcg agctgatgta gaggtcctgg atgtcgtcgg
     1801 gcgagggctt caggaccacc tggtactgat agtagtgctg caggcggttg ggattgtcgc
     1861 cgtagcgccc gtccttggga cgccgcgaag gctgcacgta cgctgccttc cagggttcgg
     1921 ggccgatcgc gcgcaggaag ctggcggtgt ggaaggtgcc tgcgcccatc tccatgtcgt
     1981 agggctgcat cagcacgcag ccctgcttgt cccagtaccc ctggagcttg agaatcagtt
     2041 cctggaacgt gagcatggcc tcgctttcga agcgctcgat tttaccggct ttcccatgtc
     2101 ggaccaacgc ggcgccgact cgcgagccag gccgcggcgg cgagcgagag cgcgcacagc
     2161 acgagcgcgg gcgcgttcgc ccagcgcacg tagggcgtcg cgccggagaa gccctgcgcg
     2221 ctcgcctgga gcgcgccctc ggtgaattgc ggcaggcgcg agcgcacgcg gccgcgctcg
     2281 tcgatcacgg ccgtgacgcc cgtgttcgtg gcgcgcagca tgtaacggcc ggtctcgagc
     2341 gcgcgcatcc tcgacatctg caggtgctgc gggggcgcga gcgagtcgcc gaaccatgcg
     2401 acgttggacg cgttcacgag cagcgtcgcc tcggggagct ggcgcacgat ctcctcgccg
     2461 aacgcgtcct cgtagcagat gtcgggcgcg acccgctggc cggcgacggc gagcggccgc
     2521 ggccgctccg ggccgcgcga gaagtcggcg agcgggatcg ccacggtgtc gacgatccag
     2581 cggaacccgg gcgggatgaa ctcgccgaag ggcacgaggt gcgacttgct gtagatctgc
     2641 ggcggcgaga cgccgaggct gatcaggctg ttgaagtagc gtccttcggc gtcgcgcacc
     2701 ggcacaccga cgagcaggtc ggcgtgcttg tcgcgcgcga ggcgcgcgag ctggttccaa
     2761 tacgcggggt cgacggtgtc gaagaaccgc ggcacggcgg tctcgggtag aacgatcagc
     2821 gtcgcgtcgc tcgcttccac caggcggcga taggtctcga gcgtcgccgc gtagcgctgc
     2881 tcctggaact tgaggtcctg cgcgatgttt ccctgcagca gcgagacgcg gaggggggcg
     2941 ccttcgggtg cggtccacga ggcggatccg aggccgtacc ctgcggcgat cacgaccgcg
     3001 atgccgcagg cgcccgcgat ccgcgcgcgc ggctcgccgg ccacgaccag gaacgcgaga
     3061 cctgcgacga gcgccgtggc ccacgacacg ccgtacaccc cgagcaccgg ggcgagcccc
     3121 gcgagcgggg aatcggtctg gctgtagccg atcgcgagcc aggggaatcc ggtgaagacc
     3181 cagccgcgca gccattcggc gagcgtccac agcgccggga aggcgagcag cgccgcagta
     3241 gccgggcgcg cgcgcaggcg cgcgagacac caggccgcgg ccgcgggata gagcgagagg
     3301 atcgcgcaga agcccagcgt cgcgacaccc gcgagcggaa ccggcatcgt tccgaagtcg
     3361 tgcaagctca cgtagaccca cgaggcgccg accccgaaca ggccggcacc gaacgcgaag
     3421 ccgagcgcca gcgcgccgcg cggcgtggct ccgcggcaga gcccgagcaa tagcgcgagc
     3481 gagagcaccg gaagcggaaa gatacccgcg ggcgcgaacc cggcgaccgt cgccgcgccc
     3541 gagccgaagg cgaccgcgac cgcggcgcgc ggcggaagct gcccgagcct caagcccggc
     3601 ccggcaggcg ctgcacctgg agcgcgtgca gccgccggct gtcggcgcgg agcacctgga
     3661 agcgcaggcc gccgatcgtg acggactcgc cgcgcttcgg caggcgaccg aagcggtcga
     3721 tgatcaggcc gcccacggta tcgaaatcct cgtcggagaa gtcggtgccg aacgcgcggt
     3781 tgaaatcgcc gatctcggtc tgcgccttga cccgccagtg cccgctagac tcgcgcacga
     3841 tgttgtcctc ggactcgtcg aagtcgtact cgtcctcgat gtcgccgacg atctgctcga
     3901 gcacgtcctc gatggtgacg agccccgcga cgccgccgta ctcgtcgacg acgatcgcca
     3961 tgtggttgcg gttggcacgg aactcgcgca gcagcacgtt cagccgcttg gactcgggca
     4021 cgaacaccgc gggccgcagc atgtcgcgga ccttgaactc ctcctcgccc gcgtagtagc
     4081 gcagcaggtc tttcgcgagc aggatcccga tcacgtcgtc gcggtccttg tcgatcaccg
     4141 ggaagcgcga gtgcgccgtc gcgatcacgt gcgggatgaa cttgtcgggc gtctcgttgg
     4201 cgtcgatcac gtccatctgc ggacgcggca ccatgatgtc gcgcacctgc agctcggaca
     4261 cctgcagcac gccctcgatc atcgacaggg catcgctgtc cagcaggtcg tgctcgtacg
     4321 ccgagtgcag gaggttgacg agctgctcgc ggtcctcggg ctcgcgcatc agcagcgccg
     4381 acagccgctc gaggagcgag ggtttgtggt cttgtgcgtc gtccatgcgt ctggggcagc
     4441 acgccgcggc ggccgcggtc gcgcgctcta gcccggatag gggtcaggat agccgagttt
     4501 ggccaggatc tgcgtttcga gcgcttccat gcgccgggcg gcccgcggcg cctcgtgatc
     4561 gtatccttgc aggtgcagca ggccgtgcac gaccagatgg gcatagtgcg cttcgagcgc
     4621 cttgccctgt cgcaacgcct cgcgccggat gacgggcgcg cacagcaaga ggtctcccgc
     4681 gacgccggcg cggccgcgat cgtacgcgaa gctgagcacg ttcgtcgccc ggtcgcgccc
     4741 gcggtagacg cggttcagcc ggcgtccttc cgcctcgccg accacccgca ccgtcacgtc
     4801 ggccggtcgc atgatcgccg cgcgcgccca gcgccgcacc ttggcgcgcg acgggaagcg
     4861 cgagcgccgg gcgacgagct gcacgtcgag cgtgagcgcc ggcgccttcg gcacgtcagc
     4921 ccttctgctc gtacgcgtcg acgattcgct gcacgagcgg attgcgcacg acgtcgtcgc
     4981 tcttgaacgt cgagaacgcg atcccgcgca cgtcctgcag gatgcgctcc acctcgacca
     5041 acccgctctt ctggccgcgc tgcaggtcga tctgcgtcac gtcgcccgtg acgacgacct
     5101 tgctgccgaa gcccatccgc gtgaggaaca tcttcatctg ctcgggcgtg gtgttctgcg
     5161 cctcgtcgag gatgatgaag gaatggttca gcgtgcggcc gcgcatgtac gcgagcggag
     5221 cgagctcgat cgcgccgcgc tcgaacagct tgccgacgcg gtcgtagccg agcaggtcgt
     5281 agagcgcgtc atagagcggc cgcaggtagg ggtcgacctt ctgcgcgagg tcgccgggaa
     5341 gaaacccgag gcgctcgccc gcctcgaccg cggggcgcac gagaacgatg cgcttgaccg
     5401 cgtcgcgctc gagggagtcc acggcacaag cgaccgcgag ataggtcttg cccgtgcccg
     5461 cgggtccgat cgcgaacgtg atgtcgtgct cgaggatgtt ctggatgtac tgtcgctggc
     5521 gcggggtgcg tccgcgcagg tcgccgcggc gggtgagcag cggcgtgtcc tcggtctcgt
     5581 ggctgcgcgg atcggtgcgg aactcgacca gcccgagctg tacgtcgtcg acggtgagac
     5641 cgcgttttgc gtcgccatag aacttctgca gggcttcggc ggcgcgtgcg gcctggcccg
     5701 gcgcgccgcg taccgtgaag cgctcgccgc gccgcgagag ggtcacatcg agcgcggtct
     5761 cgatctgccg caggttctcg tcgagcggcc cgcaaaggtt cgcgagccgc tggttgtcga
     5821 gcggcgacag gatcacctcg agcggcttga tcttcgcgga agtcatacgg tggcgcggca
     5881 gatcgcggct cagcgcgccg cctggatctc tccgcgcagg gtatgcgcca gcaccgcggt
     5941 gatggtcgca tcgacgaacc ggccgacgag gttcgcctcg cccggcatgt tgacgacccg
     6001 gttattggcg gtgcgcccgg ctagctcgcc ggcgtcgtgg cgcgatgggc cctcgaccag
     6061 gatgttctgg cgcgtgccga ccatggcctc ggagatcgcg cgcgcgtggc tgtcgagccg
     6121 tgcctgcagc gcgtgcaggc gctcgagctt gacccgcgcc ggggtttcgt cggccagctc
     6181 cgcggccggc gtgcccggcc gcgggctgta gaggaaggag aacgagctgt cgaagcgcag
     6241 ctcgtcgacc agcgccagcg tcgcctcgaa gtcgcgctcg gtctcgccgg gaaagccgac
     6301 gatgaaatcg gatgtgatgc agacgtccgg ccgggccgcg cgcagccggc gcacgatcga
     6361 cttgtactcg aggaccgtgt agccgcgctt catggccgcg agcacgcggt ccgatcccga
     6421 ctgcacgggc aagtgcacgt gcggcaccag ctcgtcgatg ccggcgtgcg cgtcgaccaa
     6481 gcgttgagtg aactcccgcg ggtgcgacgt ggtgtagcgg atacgatcga ttcccgggat
     6541 ctcggcgatg tattcgagga gcagcgcgaa gtcggccgcc tcggcgtcca ccgtcccgcg
     6601 ccaggcgttc acgttctgtc ccagcagcgt cacttctctc acgccttcct ccgcgaggtc
     6661 ggcgatctcc gtgaggacgt cctcgagcgg gcgcgagacc tcctcgcccc gggtgtaggg
     6721 gacgacgcag aagctgcagt atttgctgca gccttccatg atcgagacga aggccgtcgg
     6781 cccgtcgacc cgcggcgggg gcagcgcgtc gaacttctcg atccgcggga acgagacgtc
     6841 gacctgcggc cgtccggtcc gccgcctggc ctcgatgagc gccggcagac gatgcaaggt
     6901 ctgggggccg aacaccacgt cgacgaacgg agcgcgcgcg acgatggccg ctccttcctg
     6961 gctcgccacg cagccgccga cgccgatcag aaccccgggc cggtcgcgct tgagatgcct
     7021 gatgcgcccg agatcgctga ataccttctc ctgggccttc tcgcgcaccg agcaggtgtt
     7081 gaacaggatg agatcggcat cgtcgggcgc ggcggcgggc tcgtagcctt cggcggcttc
     7141 gagcacgtcg gccatcttgg ccgagtcgta ctcgttcatc tggcagccga acgtccggat
     7201 gtagaccttc ttcgccacgg atcgctagcg cctggcagcg ggccgcgggc gagcccgctc
     7261 ttcgtcggac aggagccaga cgcgaaagac ctcgccgccg cgatcgagga gatagtccgc
     7321 ccaggcgcct tcgcgcggca tcgcggccgg gacgatgatc aggttgtgcg agtcgcggat
     7381 ggtggccccg ggcgcgagcc gcgccggggc gccgtcgatc gacaccaacg cctcgcgctc
     7441 gtgcttcagg aagccgcgct tggcgtcggc cgggatcgtc cgctgctggg ccgccgccgg
     7501 gaggacggcg agcgcgatcg acagcagcaa cagcgcgcgg ctcggagcca tggacctcgc
     7561 gggaaaaaag aatggtggcg aatcagggac tcgaaccccg gacctgcgga ttatgattcc
     7621 gtcgctctaa ccagctgagc taattcgccc ccgaagcgga aattatcctt tgtagtccaa
     7681 tagttgtcaa tctagaatac cggcatgcaa gttgatgtcg ccattgtcgg cggaggcttg
     7741 gtcggcgcga gcctcgccgc ggcgcttgcc gggtccgggc tcgagatcgc gctgctcgat
     7801 cagtcgcctc ctccagagcc cgcgcagggc tgggacgcga ggatttacgc gctatcgccg
     7861 gcgagcgtcg cctttctgcg cgcggccggc gcctggcagc tcctcgaccc agcgcgctcg
     7921 acgccgatcc accgcatgcg cgtgttcggc gacgacggcg tctccgagct cgggttctcg
     7981 gcgtacgagg ccggtgtggc cgagctcgcg gtgacggtcg aatcgggccg tctggcgcgg
     8041 gcgctgtgga gccgcctcga cggcgcgtcc ggcgtgcggc gcttgtgccc ggcttcgccg
     8101 gttgcgctcg agcgctcgcc cgaggaaatc atcttgcgcc tggcctcggg cgaggcgctg
     8161 cgcgcgcgcc tttgcgtcgg ggccgacggc gcgcggtcgt gggtgcgggg cgcggccggc
     8221 atgacggcct cgacgcgtcc ctacggcgag cgcggagtgg tcgccaattt cacgtgcgag
     8281 gtgccgcacc ggaacacggc cttccagtgg tttcgcggcg acggcgtgct ggcatggctc
     8341 ccggttgcgg accacgtcgt gtcgatcgtc tggtcgaccg ccgagacgcg cgcgcgcgag
     8401 ctgctcgcgc tttcgcccga tgccctgtgc gacgcggtgg ccgcggcggg cgctcgggcg
     8461 ctgggtgccc tgacgctcgt cacgccgccg gcatcgttcc cgctggagcg cctgcatgcg
     8521 tcgcgctcgg cgcgcgagcg cgtggcgctg ctgggcgacg ccgcgcacgt cgtgcatccc
     8581 ctggcgggcc agggcgtgaa cctcggtttc gccgatgccg ccgcactcgc cggcctgctg
     8641 cgcgggatgc gcggacaccg cgatgcgggc gacgcctcgc tgctgcgccg ttttgagcgc
     8701 aaacgcgccg aggatatact ggcgatgcgc tgggtcaccg acggcctcca cgggctgttc
     8761 ggcgccaggc atccggccgc ggcgcgcata cgtaatctcg ggctgagcct gaccgactcg
     8821 attcctctcg tcaaatcgct gcttgcgcgt cgcgccatgg gcgcggccga cttctccaag
     8881 gacgctccat gatcatccgc tcgttcgccg ccgtcaccct tgccctcgcc gcgctctcgg
     8941 cgagcgccga cgaggcctcg atcagacgcg cgatcgaagc gcgcttcaac ggcgtcgcgg
     9001 tcgagagcgt caccaagacg ccttactcgg gcctctacga agtcgtgatc ggcgacgaga
     9061 tcgtctacac cgacgacaag gtcacctata tcttcaccgg caacatcatc gacgcgaaca
     9121 cgcggcgcaa cctgaccgag gagcgccagt cgcagctgct cgccgtgaac ttcaaggacc
     9181 tgccgctcga caatgcgatc aagaccgtcc gcggcagcgg caagcgcgtg atggtcatct
     9241 tcgccgaccc gcgctgcggt ttctgcaagc agttcgagca gaatctctac aagctcaccg
     9301 acgtcaccat ctacacgatg ctctaccccg tgatcgcgcc ggactcggcc gagatctcgc
     9361 gcaacatctg gtgctcgaag aacccgagcc aagcctggct cgacacgatg ctgaagggcg
     9421 tgctgccgcc gacgaacacc tgcgcgacgc cgctcgacaa gaatctcgcg cttggcaagc
     9481 gcctgcgcgt cagcggcacg ccgacgacct tcctgccgaa cggccagcgc atcgtcggcg
     9541 cacgcttccc cgagctggtg caggcgctcg accaggcgtc gaagtaggcg cctcacgacg
     9601 ccggaggctt cattcctttc ggcagcagca cccaaagcgc gccctgctgg cgcatgcgcc
     9661 cggcgagcgc tcccgcctcg gacccgaatc cccagaaata atcggcgcgt accgggccgc
     9721 ggatcgcgcc cccggtgtcc tgcgcgatga ccagccggtt gagcggcaga tcgctcgagg
     9781 gccacgtcgt gccgaggaag gcgggcgcgc cgagcggcac gtagcgcgga tcgaccgcga
     9841 tcgagcgctg tggcgtgagc gggacgccga gcgcgccgat cgggccgacc gacggatcgg
     9901 tgagcggcag ctcgcggaag aacacatagc tcggattctg atcgagcagc tccggcagcc
     9961 ggtccggatg cgactcggcc caggcgtgga tcgcctgcat cgacgcctcg ccgggctgca
    10021 gatcgccgcg ctcgacgagg gtgcgtccga tggattggta ggggtgcccg ttctggtcgg
    10081 cataaccgac gcgcatgacg tcgccgttgt cgagacggat gcggcccgac ccctggatct
    10141 ggaggaagaa ggcctcgacc gcgtcgtcga cccagaccag ctccttgccg gcgaccggag
    10201 cggctccctt ctcgatttcg gcgcgcgacc agtaggggac gaccttgcgg ccctggagcc
    10261 ggccgcgcag acgcatgttc ctgaggtccg ggttcaccgc ggaaagatcg atggtgagca
    10321 agtcgtccgg cggcgcatag agcggcacca cgaagggcgg ctggcgcacg cggctccctc
    10381 gcagcaacgg ctcgtagtac cccgtgatta gtccttcggt gcgtccgtcc ggagcggcga
    10441 ggcgccaggg agcgaagcgc gactcgatga aggcacgcgc ggtcgcgtcg tccggcgcgg
    10501 gcatcgacgc cgcgtcgagg cacgccgcgc gccaggcgtc gcggaagcgc agcgcctggc
    10561 acgacgcgag cagcgcgctc catgcggccg cctgctcgcc gtcggtccaa ccggggatgt
    10621 ccgggtacgc gacctcctgg tagagctcga cgcgcggcgc gggcggagcc ggccggccgc
    10681 acgggcagcg gtcggtcgcg acgggagagg gcgcgctcgc gcagccggcg atgagggctg
    10741 cggcgaccag cacggcagcg cgggacggcc ttatcatgca gtcgtggact ccggactttg
    10801 gatcatcgta ctcgaagcgg gtctcgcgct cgcgctgctg ttgttcatcg tctggtggac
    10861 cgttccgaag cggcgccgcg acccggaatc cgacgcgaaa tccgaggagc gcacggaaaa
    10921 acgctgacgg cgcgccggcg cgtcagtgca ggacgcgcgg caccgagagg gtgaactccg
    10981 ggatgtcggc gtcgaactgc gtgccgtcct cggcgcgcat ctggtagctg ccgcgcatcg
    11041 tgccgacggg agtggcgatc gcggtcccgc tcgtgtactc gaacgactct ccgggcttga
    11101 ggttcggctg tgcgccgacc acgcccagcc cccggacttc ctgcacctgg ttgcgcgcgt
    11161 cggtgatgac ccagtgccgg gatacgagct gcgcgctcac ggtcccggtg ttgcggatgg
    11221 tcatcgtgta ggcgaacacg aagcggctgt tctgctcgtc ggactgctcc gggatgaact
    11281 gcgtctgcga cgagacgagg acttcgtatt tcttgtcggc catgacccga tttcacacga
    11341 aatcaccagg gctgacaagg cgcgaggcgc gcggcgcgac acgcgccgat taccagtaaa
    11401 attcgtttca tggccgcgcg cgttttccgc atcgccccga gcatcctgtc ggcggacttc
    11461 gcacgcctcg gcgacgaggt gaagacggtg gtgtcggcgg gcgccgacat gatccatttc
    11521 gacgtgatgg acaaccatta cgtgcccaat ctcaccgtcg gcccgctcgt gtgccgggcg
    11581 atccgtccgc tgacgcaggc ggacatcgac gtgcacctca tggtgaagcc cgtggatcgc
    11641 ctggtgtccg atttcgcgaa ggcgggcgcc aatatcatca gcttccatcc cgaggcgagc
    11701 gagcacgtcg accgcaccgt cggcctgatc cacgattcgg gctgcaaggc cgggctggtg
    11761 ctcaatcccg cgacgccgct cgtggtgctg gaccacgttc tgcacaagct ggacctcgtc
    11821 ctcctcatgt cggtgaatcc cggattcggc ggacaggaat tcatcccgca gacgctcgag
    11881 aagctgcgcg ccgcgcgccg gcgcatcgac gaagcgtctc aggcgagcgg tcgggcgatc
    11941 ctgcttgagg tcgacggcgg catcaaggtc gacaacatcg ccgcggtcgc gcgcgcggga
    12001 gccgacacct tcgtcgcggg ctcggctatc ttcggatcgg gcgactaccg cgcgacgatc
    12061 gcgaagatgc gcgaggcgct gcagacggcg gcttagcgga tggccgagct gctcgagcgt
    12121 ctgcgccggg agatcggcga cgccgcggtg ctcgccgccc cggccgactg ggcgccttac
    12181 gcgatcgact ggcgccggcg ctactccggt cggccgctcg ccgtcgtgaa gccggcgtcc
    12241 accgcggaag tcgcggctgt cgtgcgcgcc tgcgccgagg cgcgcgcggc ggtcgtgccg
    12301 caggccggga acacgagcct ttgcggcgcg gcgacgcccg acggcagcgg cactcaggtc
    12361 gtactgaacg tgtcgcgact caatcgcgtc cggagcatcg acgcggcgaa caacgccatg
    12421 accgccgagg cgggctgcgt gctggcgtcg ctgcacgacg ccgcgcgcgg cgcgaatcgc
    12481 ctcttccccc tgacccttgc ggcgcagggc agctgcgagc tcgggggcaa cctctcgacc
    12541 aacgccggcg gcacgggcgt cctgcgctat gggaacgcgc gcgatctcgt gctcgggctg
    12601 gaggtggtcc tgccctcggg tgaggtgtgg gacgggttgc gcgcactgcg caaggacaac
    12661 accggctacg atctcaagca gctgttcatc ggcgccgaag gcacgctcgg cgtgatcacc
    12721 gcggcggtcg tgaagctctt cccgttgcct cgctcccagg cgaccgcggt ggtggcgctg
    12781 ccggacgccg agcgctcgct cgcgctgctc gggacggcgc agtcgcattg cggcgagcgg
    12841 ctcacggcct tcgagatctt ctcgcacgcg tgcctcgagc tcgtgctcgc gcacttcccg
    12901 ggcacgtcgg cgccgttccg ctcgccttac ccgcagtatg cccttctcga agtttccgat
    12961 tccggcgcgg gcgcggacgc ggcgctcgaa gcggcgctcg ccgaagcgat ggagaagggc
    13021 agcgtcctgg acgccgtggt cgcgcagagc gagtcgcagg cgcgcgcact gtgggcgctg
    13081 cgcgaaaaca tctccgaggc gcaggcgcgc gaaggtccga acatcaagca cgacgtttcg
    13141 gtgccggtct cgagcatccc cgcgtttgcc gccgcgaccg gcgcggagct tgcccgcgcc
    13201 ttccccggct cgcggctcat cgtgttcggg catctctgcg atggcaacct gcactacaac
    13261 ctctcgccgc cgccgggcgt cagcgccgag acgttcatgg gcaacctgga cgcggtgaac
    13321 cgcgtcgtgc acgacagcgt cgcgcgcttc cgcggctcga tcagcgccga gcacggcatc
    13381 gggcggctca agcgcgaaga gctcgcgcgc tacaagacgc cgctcgagct cgacctgatg
    13441 cgcgccgtca agcgcgcgat cgatccgctc gggatcatga accctggcaa ggttttctag
    13501 gcgccctgga caacggtcgg ggcgccgttg taaagtgagt tctctcggaa attcaaggca
    13561 ctcggtcgtg ctctacggga acaccggaac gagaatgcag ggacgcgcct ggcgctggtg
    13621 gcgccgctga tcgcgctcgc gcgcgtcgtc gcgacgcgca ccccgcaggt cccggaaatg
    13681 ttttcgtgga gccgtcgatg accgaagaga agttcaagca gctcgccgcg cagggctaca
    13741 accgcgttcc cgtcgtcgcc gaaaccttcg cggacctcga caccccgctc tcgatctacc
    13801 tcaagctcgc caaccgctcg aacacctacc tgctcgagtc ggtcgtcggc ggcgagcggt
    13861 tcggccgcta ttcgttcatc gggctcgcga cgccgatccg catccgcgcg gtcgggcgcc
    13921 tggtcaccgt catgcacggc gacgcggtca ccgagcgctc gcagtccgga gatccgctgc
    13981 agttcgtgcg cgagttcctg tcgcgataca agatcgcgga gccgcccggg ttgccgcgct
    14041 tctgcggcgg gctggtcggc tatttcggct acgacaccgt gcgctggatc gagccgaagc
    14101 tctcgggcgg ctggctcaag cgcgatccgc tcggctgtcc cgacatgctg ctgctcgtgt
    14161 cggaggagct cgccgtcgtc gacaacctca ccggcaagct gtacctcgtc gtattcgccg
    14221 atccgtccaa gccgcgcgcc tgggacgacg cccaggcacg gctgcgcgag ctgctcggc
//
LOCUS       DATDMW010000318        10167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_592644, whole genome shotgun sequence.
ACCESSION   DATDMW010000318 DATDMW010000000
VERSION     DATDMW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592644"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="VI319_13385"
     CDS             <1..176
                     /locus_tag="VI319_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HEY6864883.1"
                     /translation="LWQLAYTELYFTPTLWPDFDARELDRAIAWYGERERRFGRTSEQ
                     LAEGAADGRRRIA"
     gene            211..1029
                     /locus_tag="VI319_13390"
     CDS             211..1029
                     /locus_tag="VI319_13390"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY6864884.1"
                     /translation="MMTTRILTAAALIAVVVAALFFLPRTGWLVLAAIMLAQGAWEWG
                     GLARLGAAARAIYTMALVIALLWLAYDPDPVSTRWAYYVAAVFWVIVAPWWLWRRPAF
                     GGPGAPLAAGVVVLVPAFLALVLLRDDGGPLALLALMMSIWISDTAALFAGRRFGRRK
                     LAPEVSPGKTWEGVYGALVAVAVYGALTGWGFMRSGLIAPRASLFWSWILMLLAIAAA
                     GVVGDLFESQMKRAAGVKDSGNLLPGHGGWLDRIDAQTAALPVAALIFSAFQAA"
     gene            1026..2222
                     /gene="ispC"
                     /locus_tag="VI319_13395"
     CDS             1026..2222
                     /gene="ispC"
                     /locus_tag="VI319_13395"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY6864885.1"
                     /translation="MKTQNIAILGATGSIGASALDVIARHPERFRAWALTANRRVDAL
                     AALCAKHRPAWAGIADARLAPELERRLREAGVPTQVAAGADGLAALAEGAEVDTVIAA
                     IVGAAGLRATLAAAAAGKKLLLANKEALVCAGPIFMETVRRHGATLLPVDSEHNAVFQ
                     CLPPFASDGLAQKGIRRIVLTASGGPFRRRARAELQRVTPDEACAHPNWSMGRKISVD
                     SATMMNKGLEVIEAHWLFGAAPEQIEVVIHPQSVVHSMVEYADGSVIAQLGNPDMRTP
                     IAQALAFPDRIDAGVEPLDLARIGQLGFEQPDLERFPALRLAYQALRAGGTAPAVLNA
                     ANEVAVAAFLERRLSFLGMTAVIEETLGSTALAPLATLQDVLAADAAARAHADQIVHR
                     SYSNAA"
     gene            2219..3589
                     /gene="rseP"
                     /locus_tag="VI319_13400"
     CDS             2219..3589
                     /gene="rseP"
                     /locus_tag="VI319_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY6864886.1"
                     /translation="MSLLFTIAAFIVALGVLIVIHEYGHYLVARICGVKVLRFSVGFG
                     KPLWTRRAGPDRTEWVIAALPFGGYVKMLDEREGPVAPHELHRAFNRMSVWRRILIVI
                     AGPLANFVLAIVIYWGLFLHGVQEVKPIVAAPEPGSIAAASGLKSGETIVAVEGEPVS
                     SWQDLRWRLLQLAIDRQPVRVETIDRERNANQRTFDLGRFDIDDLDGDPLTRIGLSLW
                     MTRPDVPPVIDSIEPKSPADAAGLRQGDRVAAVDGREIRRWDELVPVVRASPGKALRL
                     RVLRGADTIELTVRPDALQQNGRALGRIGAGPKVDPEALKGLFARVSYGPITALSKSL
                     DRTWETSVFSLSMLGKMITGKVSWKNLSGPVTIADYAGQSARQGFGAYVVFLALISIS
                     LGVLNLLPIPLLDGGHLLYYLVELLKGSPVSERAMELGQRFGLTVLLFLMVFALYNDI
                     NRQLAG"
     gene            3609..5897
                     /gene="bamA"
                     /locus_tag="VI319_13405"
     CDS             3609..5897
                     /gene="bamA"
                     /locus_tag="VI319_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY6864887.1"
                     /translation="MLLLRAAFVAALFTTLLARAAWAFEPFTIRDIRIEGIQRIEAGT
                     IFSYLPVKVGEQMTDEKAAQAIRALFGTGFFRDVRLDVQGNVLVVSVDERPAIASIEF
                     SGMKEFDKDAVKKSLREVGFQEGRIFDRAMLEQAEQELKRQYLGRGKYGVQVTTTATP
                     LDRNRVAVTFSIDEGEVAKIRKINIVGNKAFSEKELLDQMVLTTPGLMTWYTKNDQYS
                     RQKLQGDLESLRSYYLNRGFVDFNIDSTQVSITPDKRDIYITVNVTEGEVYTVTDVKL
                     GGDLLLPEAELRKLVTVKPGEPFSREKLNDSTKAITERLGNEGYAFANANAVPAIDKD
                     KRTVAFTIFVDPGRRVYVRRINIVGNTRTRDEVIRREMRQLEGSFYDAQKLQLSKQRI
                     DKTGYFSEVEVETPAVAGTTDQVDVTVRVKERPTGALLVGVGFSSVDRLIFQGSISQS
                     NIFGTGNTFSFQANTGTVNKTVSMSFTDPYWTIDGVSRGFDVYRRAIDATTLGIGNYK
                     TTTLGGGLRFGVPFTEYDTLFFGVAYEQTKLQLDPVLSPQRFLNFESEFGDEYSAVIE
                     TAGWVRDTRDSAIWPTRGRIQRGNVEVATSPGDLEYWKTTYQNQSFFPITRDVTLMLN
                     GVVGAAHGYGGKPLPFFKNFYLGGVGDVRGYRAASLGPRDVDGSSLGGSKKTNASAEL
                     LFPFPGTGKDRSMRLGYFVDGGQVYGEDEPVKFNTWRYSTGFSFAWNSPLGPLKFSMA
                     WPLHDQPDDHIQRFQFQLGTIF"
     gene            5969..6481
                     /locus_tag="VI319_13410"
     CDS             5969..6481
                     /locus_tag="VI319_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY6864888.1"
                     /translation="MRHSATLILLSGLLVSGPVFAQAKIGFVSLDRILHDAPVAQRAQ
                     KKIEAEFSKRDQELGKMSEQLKKQQENLEKNGVTMSDADRQKRERELNDLNREFQRKQ
                     REFREDLNTRRNDELAGVVDRANKAIRQIAETEKYDIIFQEAVWANPRIDITDKVIKA
                     LDDSRPAAAK"
     gene            6481..7521
                     /gene="lpxD"
                     /locus_tag="VI319_13415"
     CDS             6481..7521
                     /gene="lpxD"
                     /locus_tag="VI319_13415"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY6864889.1"
                     /translation="MAALRLSDIVARVGGELIGDPDITVQRVAPLQVAGPEDLSFLAQ
                     AKYRPQLRETRAAAVVLGRAERDATPLPRIVCDDPYLYFARVAQLFAPADAVSAGVHP
                     SAVVEADATVPASASVGPGAYIGSRARLGERVVVGPGCHVCARVEVGDDSRLNALVSV
                     YAGCRIGRRTIVHSGAVIGADGFGMARDGERWTKIPQVGAVVIGDDVEIGANTTIDRG
                     ALEDTVIEDGVKLDNQIQIGHNVRVGAHTAMAGCVGVAGSARIGRHCTVGGAGMILGH
                     LEIADHVNISAGTLVAKSIRRPGTYTGIFPIAENKEWARIAAHLRRLDELAERVRELE
                     RRLTERKRGKKK"
     gene            7527..7961
                     /gene="fabZ"
                     /locus_tag="VI319_13420"
     CDS             7527..7961
                     /gene="fabZ"
                     /locus_tag="VI319_13420"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY6864890.1"
                     /translation="MSIHEVLEYLPHRYPMLLVDRVLELEPGKRILALKNVTFNEPFF
                     PGHFPQHPVFPGVLVIEAMAQASAILTFKTLGSKPRPDSVYYFVGIDEARFKKPVFPG
                     DQLLLDVSIERNVRGIWKYTGQARVGDQLVAEGRLMCTNRDL"
     gene            7958..8728
                     /gene="lpxA"
                     /locus_tag="VI319_13425"
     CDS             7958..8728
                     /gene="lpxA"
                     /locus_tag="VI319_13425"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY6864891.1"
                     /translation="MIHPTAVVHPGATLGPRTTVGAYSLIGEHVEIGEGTSIGSHVVI
                     EGHTRIGAGNRIFSHSALGGAPQDKKYAGEPTRLEIGDRNTIREFCTFNTGTVQDAGV
                     TRIGSDNWIMAYVHIAHDCDVGSNTIFANNAALAGHVRVGDWAILGGYTMVHQFVSIG
                     AHSMTAIATVLLLDLPPYVMAAGNTAQPHGINSEGLKRRKFSPESIARIKSAYRTLYR
                     EGLKLEEARAELQKMAEASPEIAPMVEFLAASRRGITR"
     gene            8729..9901
                     /gene="lpxB"
                     /locus_tag="VI319_13430"
     CDS             8729..9901
                     /gene="lpxB"
                     /locus_tag="VI319_13430"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY6864892.1"
                     /translation="MMAAGGQPLVAMVAGEASGDFLGAHLMTALRKRFPGLRFAGIGG
                     PRMEGLGFDSWYPMERLAVRGYAEVLRHYPGLAMMRRALGRRLIAARPALFVGVDAPD
                     FNLGLERRLKAAGIPTAHYVSPSVWAWRGKRVRTIARAVSRMLVLFPFEEEIYRAAGV
                     PVTYVGHPLAEQIPMEYDVELAREQLKLPPTRRIVVLMPGSRQSEVRYMAPAFVRAAM
                     CIAEQLPAAHFLVPLVSRETRAIFEQAMYDAGNPELPLTILFGHAREAIAAAHAVLVA
                     SGTATLEAALYRKPMVIAYKMAKSTHRIMSRMAYQPWIGLPNIIAGEFIVPELIQDEA
                     TPENLAQALLNALADPVVARRLPERLARLHELLRRDSAAIVAEALSPWLARASSAA"
     gene            9871..>10167
                     /locus_tag="VI319_13435"
     CDS             9871..>10167
                     /locus_tag="VI319_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY6864893.1"
                     /translation="MARPRLVCGIDEAGRGPLAGPVYAAAVVFDPRRRVPRGIHDSKV
                     LASGERERMAGVIREAAIGWCVAYATVEEIDRLNILRASLLAMRRAVQGLPLIPD"
BASE COUNT         1569 a         3557 c         3517 g         1524 t
ORIGIN      
        1 tcctctggca gctcgcgtac accgagctct acttcacgcc cacgctgtgg cccgatttcg
       61 acgcgcgcga gctcgaccgg gcgatcgcct ggtacggcga gcgcgagcgc cggttcggcc
      121 ggaccagcga gcagctggcg gaaggcgcgg ccgacgggcg ccggagaatc gcgtaggctc
      181 cgcgccgcac cgcggagccg cgggctcgcc atgatgacca cgcggatcct gaccgcggcc
      241 gcgctcatcg cggtggtcgt cgccgcgctc ttcttcctgc cgcggaccgg atggctcgtg
      301 ctcgcggcga tcatgctggc gcagggcgcc tgggaatggg gcggcctcgc gcggctcggt
      361 gccgccgcgc gcgcgatcta caccatggcg ctcgtgatcg cgctgctctg gctggcctat
      421 gaccccgatc cggtttccac gcgctgggcg tactatgtgg cggcggtgtt ctgggtgatc
      481 gtggcgccct ggtggctgtg gcgccgcccc gccttcggcg gaccgggcgc gcccctcgcg
      541 gccggagtcg tggtgctggt ccctgccttc ctcgccctcg tcctgctgcg cgacgacgga
      601 ggtccgctcg ccttgctcgc tctgatgatg tcgatctgga tctccgacac ggccgcgctc
      661 ttcgccggcc gccgcttcgg gcggcgcaag ctcgcgcccg aggtcagccc cggcaagacc
      721 tgggagggcg tctatggagc gctcgtcgcc gtcgccgtgt acggggcgct caccggctgg
      781 gggttcatgc gctccgggct catcgccccg cgcgcctcgc tgttctggag ctggatcctc
      841 atgctgctcg ccatcgcggc cgcgggcgtc gtcggcgacc tgttcgagtc gcagatgaag
      901 cgcgccgcgg gcgtcaagga cagcggcaac ctcctgccgg ggcacggcgg ctggctcgac
      961 cgcatcgatg cgcagaccgc cgcgctcccg gtcgccgcgc tcatcttctc ggcgttccag
     1021 gcggcatgaa gacgcagaac atcgcgatcc tcggcgcgac cggatccatc ggcgcgagcg
     1081 cgctcgacgt gatcgcccgg catcccgagc gcttccgcgc ctgggcgctc accgcgaacc
     1141 ggcgcgtcga cgcgctcgcc gcgctgtgcg ccaagcaccg tcccgcgtgg gccggcatcg
     1201 ccgacgcgcg cctcgcgccc gagctcgagc gccggctgcg cgaggccggc gtgccgaccc
     1261 aggtcgcggc cggcgcggac gggctggccg ctctcgccga gggcgcggag gtcgacacgg
     1321 tgatcgcggc catcgtcggc gccgccggcc tgcgcgcgac gctcgcggcc gcggcagccg
     1381 gcaagaagct gctgctcgcg aacaaggagg cgctggtgtg cgccggcccg atcttcatgg
     1441 agacggtgcg ccgccacggg gcgacgctgc tgccggtcga cagcgagcac aacgcggtgt
     1501 tccagtgcct gccgccgttc gcgtcggacg gcctcgcgca gaaggggatc cgccgcatcg
     1561 tgctcaccgc gtcgggcggg ccgttccgcc ggcgggcgcg cgccgagctg cagcgcgtca
     1621 cgcccgacga ggcgtgcgcc cacccgaact ggagcatggg ccgcaagatc tcggtcgatt
     1681 cggcgacgat gatgaacaag gggctcgagg tgatcgaagc gcactggctg ttcggcgccg
     1741 cacccgagca gatcgaggtg gtgatccacc cgcagagcgt cgtccattcc atggtcgagt
     1801 acgccgacgg atcggtgatc gcgcagctcg gcaatcccga catgcgcacc ccgatcgcgc
     1861 aggcgctggc ctttccggac cgcatcgacg cgggcgtcga gccgctcgac ctggcgcgca
     1921 tcggccagct cggcttcgag caacccgacc tggagcgctt cccggcgctg cgcctcgcgt
     1981 accaggcgct gcgcgccggc ggcaccgcgc cggcggtgct gaacgccgcc aacgaggtcg
     2041 cggtcgcggc cttcctggag cggcgcctct cgtttctcgg gatgaccgcc gtcatcgagg
     2101 agacgctcgg gtcgacagcg ctcgcgcctc tcgctacact gcaggacgtg ctcgccgcgg
     2161 acgccgcggc gcgcgcccac gcagaccaga tcgtccatcg ctcgtactcc aacgccgcat
     2221 gagcctgctc ttcacgatcg ccgccttcat cgtcgcgctg ggcgtcctca tcgtcattca
     2281 tgagtacggt cattacctgg tcgcgcgcat ctgcggcgtc aaggtcctgc gcttctcggt
     2341 cggattcggc aagccgctct ggacgcgccg cgccggcccg gaccgcaccg agtgggtgat
     2401 cgcggcgctg cccttcggcg gctacgtgaa gatgctcgac gagcgcgagg ggccggtcgc
     2461 gccgcacgag ctgcaccgcg cgttcaaccg catgagcgtg tggcggcgga tcctcatcgt
     2521 gatcgcgggg ccgctcgcga acttcgtgct cgcgatcgtg atctactggg ggctgttcct
     2581 gcacggcgtg caggaggtga agccgatcgt tgccgcgccc gagccggggt cgatcgcggc
     2641 cgcgagcggc ctgaagtcgg gcgagaccat cgtcgccgtc gaaggcgagc cggtgtcgag
     2701 ctggcaggac ctgcgctggc gcctgctgca gctcgccatc gaccgccagc cggtccgcgt
     2761 cgagacgatc gaccgcgagc gcaatgccaa ccagcgcacc ttcgatctcg gccgcttcga
     2821 catcgacgat ctcgacggcg atccgttgac gcgcatcggg ctgtccctgt ggatgacccg
     2881 gcccgacgtg ccgcccgtca tcgactcgat cgagccgaag agcccggccg acgcggcggg
     2941 gctgcgccag ggcgaccggg tcgccgccgt cgacggccgc gagatccgtc gctgggacga
     3001 gctcgtgccg gtcgtgcgcg cctcccccgg caaagccctg cgcctgcgcg tcctgcgcgg
     3061 cgcggacacg atcgagctca ccgtcaggcc cgacgcgctc cagcaaaacg ggcgcgccct
     3121 cggccggatc ggcgccgggc cgaaggtcga tcccgaggcg ctgaagggcc ttttcgcgcg
     3181 cgtgtcatac ggtccgatca cggctctgtc gaagtcgctc gaccgcacct gggagacctc
     3241 ggtgttcagc ctcagcatgc tggggaagat gatcaccggc aaggtctcgt ggaagaacct
     3301 gagcggtccg gtcacgatcg cggattacgc cggccagtcc gcgcgccagg ggttcggcgc
     3361 gtacgtcgtg ttcctcgcgc tgatcagcat cagcctcggc gtgctgaacc tgctgccgat
     3421 cccgctgttg gacggcggac acttgctgta ctatctcgtc gaactgctca aggggagccc
     3481 cgtatcggag cgcgcgatgg agctgggcca gagattcggc ctcaccgtgc tcctcttcct
     3541 gatggtcttc gcgctctaca acgacatcaa ccgccagctc gcgggatagc aggcgccgct
     3601 ccatgaggat gttgctgctg cgggccgcgt tcgtcgcggc gctgttcacg acgctgctcg
     3661 cgcgcgcggc gtgggcattc gagcctttca cgatccgcga catccgcatc gagggcatcc
     3721 agcgcatcga ggcgggcacg atcttcagct atcttcccgt caaggtcggc gagcagatga
     3781 ccgacgagaa ggccgcgcag gcgatccgcg cgctgttcgg caccggcttt ttccgcgacg
     3841 tgcgcctgga cgtgcagggg aacgtcctcg tcgtcagcgt cgacgagcgt cccgcgatcg
     3901 cgtcgatcga gttctccggg atgaaggagt tcgacaagga cgcggtgaag aagagcctgc
     3961 gcgaggtcgg gttccaggag ggccgcatct tcgaccgcgc gatgctcgag caggcggagc
     4021 aggagctcaa gcgccagtac ctcggccgcg gcaaatacgg cgtgcaggtc acgaccacgg
     4081 cgacgccgct cgaccgcaac cgcgtcgcgg tcacgttcag catcgacgag ggcgaggtcg
     4141 ccaagatcag gaagatcaac atcgtcggca acaaggcctt cagcgagaag gagctgctcg
     4201 accagatggt gctgacgacg ccgggcctga tgacctggta caccaagaac gaccagtact
     4261 cgcgccagaa gctccagggc gatctcgagt cgctgcgctc gtactacctg aaccgcggct
     4321 tcgtcgactt caacatcgat tccacgcagg tctcgatcac gccggacaag cgcgacatct
     4381 atatcacggt caacgtcacc gaaggcgagg tctacaccgt cacggacgtg aagctcggcg
     4441 gcgacctgct gctgcccgag gccgagctgc gcaagctggt cacggtgaag ccgggcgagc
     4501 cgttctcgcg cgagaagctg aacgattcga ccaaggcgat caccgagcgc ctcgggaacg
     4561 agggctacgc cttcgccaac gccaacgcgg tgcccgcgat cgacaaggac aagcgcaccg
     4621 tcgcgttcac catcttcgtc gatccgggcc ggcgcgtata cgtccgacgc atcaacatcg
     4681 tcggcaacac ccgcacgcgc gacgaggtga tccggcgcga gatgcgccag ctcgagggct
     4741 cgttctacga cgcgcagaag ctgcagctgt cgaagcagcg catcgacaag accggctact
     4801 tcagcgaggt cgaggtcgag accccggcgg tcgcgggcac caccgaccag gtcgacgtca
     4861 cggtgcgcgt gaaggagcgc ccgaccggcg cgctgctcgt cggcgtcggg ttctcgagcg
     4921 tggacaggct catcttccag ggctcgatct cgcagagcaa catcttcggc accgggaaca
     4981 ccttctcgtt ccaggccaac accggcacgg tgaacaagac cgtctcgatg tcattcaccg
     5041 atccctactg gaccatcgac ggcgtcagcc gcggcttcga cgtctaccgg cgcgcgatcg
     5101 acgcgacgac gctcgggatc ggcaactaca agaccacgac cctgggcggc ggcctgcgct
     5161 tcggcgtgcc gttcaccgag tacgacacgc tgttcttcgg cgtggcgtac gagcagacga
     5221 agctgcagct ggaccccgtc ctgagcccgc agcgcttcct caacttcgag agcgagttcg
     5281 gcgacgagta cagcgcggtg atcgagaccg caggctgggt tcgcgacacc cgcgacagcg
     5341 cgatctggcc gacgcgcggc cgtatccagc gcggcaacgt ggaggtcgcg acctcgcccg
     5401 gagacctgga gtactggaag acgacgtacc agaaccagtc gttcttcccg atcacgcgcg
     5461 acgtcacgct catgctgaac ggcgtcgtcg gcgccgcgca cgggtacggc ggaaagccgc
     5521 tgccgttctt caagaacttc tatctcggcg gcgtcggcga cgtgcgcgga taccgcgcgg
     5581 cgtcgctcgg cccgcgcgac gtcgacggca gctcgctcgg gggctcgaag aaaaccaatg
     5641 cctcggccga gctgctgttc ccgttccccg gcaccggcaa ggaccgctcg atgcggctgg
     5701 gatatttcgt cgacggcggc caggtgtacg gggaggacga gccggtcaag ttcaacacct
     5761 ggcgctactc gaccggcttc tcgttcgcgt ggaactcgcc cctggggccg ctgaagttca
     5821 gcatggcctg gcctctccac gatcagcccg acgatcacat tcagcgcttc cagtttcagc
     5881 ttggcacaat cttctgacgc cccggtataa tccgacgtct cgagaggtct cgagatcgaa
     5941 gcggccgccc gtggaaggag ggcacctgat gcgtcattcc gcgaccctga tcctgctgtc
     6001 gggcctgctc gtcagcggcc ccgttttcgc gcaggcgaag atcggcttcg tcagcctcga
     6061 ccggatcctg cacgacgcgc cggttgcgca gcgcgcgcag aagaagatcg aagcggagtt
     6121 ctcgaagcgc gaccaggagc tcggcaagat gtccgagcag ctgaagaagc agcaggagaa
     6181 tctcgagaag aacggcgtca ccatgtcgga cgcggaccgc cagaagcgcg agcgcgagct
     6241 gaacgacctc aaccgcgagt tccagcgcaa gcagcgcgag ttccgcgaag acctcaatac
     6301 ccgccgcaac gacgagctcg ccggcgtggt cgaccgcgcc aacaaggcga tccggcagat
     6361 cgcggaaacc gagaagtacg acatcatctt ccaggaagcg gtctgggcga atccccgcat
     6421 cgatatcacc gacaaggtga tcaaggcgct ggacgacagc cggccggccg ccgcgaagta
     6481 gtggccgcac tgcggctctc cgacatcgtc gcccgcgtgg gcggcgagct gatcggcgat
     6541 cccgacatca cggtgcagcg cgtcgcgccg ctgcaagtgg ccggccccga ggacctgtcg
     6601 tttctggcgc aagccaagta tcgaccgcag ctgcgcgaga cccgcgccgc cgccgtggtg
     6661 ctcgggcgcg ccgaacgcga tgcgacgcct cttccgcgca tcgtctgcga cgatccctat
     6721 ctgtacttcg cgcgcgtcgc gcagctgttc gcgccggccg acgcggtgag cgccggcgtg
     6781 cacccctcgg cggtggtgga ggccgacgcc acggtgccgg cctcggcgtc ggtcgggccg
     6841 ggcgcgtaca tcgggtcgcg cgcgcgcctc ggcgagcggg tcgtggtggg acccggctgc
     6901 catgtctgcg cacgcgtcga ggtcggcgac gactcgcgcc tgaacgcgct cgtgtcggtc
     6961 tatgccggct gccgcatcgg gcgccgcacg atcgtgcact cgggcgcggt gatcggcgcc
     7021 gacggcttcg gcatggcgcg cgacggcgag cgctggacca agatcccgca ggtgggcgcc
     7081 gtcgtgatcg gcgacgacgt cgagatcggc gccaacacga cgatcgaccg cggcgcgctc
     7141 gaggacacgg tcatcgagga cggcgtgaag ctggacaacc agatccagat cgggcacaac
     7201 gtgcgcgtcg gcgcgcacac cgcgatggcg ggctgcgtcg gcgtcgcggg cagcgcgcgc
     7261 atcgggcggc actgcacggt gggcggggcc ggcatgatcc tcgggcacct cgagatcgcc
     7321 gaccatgtga acatctcggc cggcacgctg gtggcgaagt cgatccggcg gcccgggacg
     7381 tacacgggca tcttcccgat cgcggagaac aaggaatggg cgcgcatcgc cgcgcacctg
     7441 cgccggctcg acgagctggc cgaaagagtg cgtgagctcg agcggcggct gacggaacgg
     7501 aagcgaggga agaagaaatg agcgcaatga gcattcacga ggttctcgag tacttgccgc
     7561 accggtatcc gatgctgctg gtcgacaggg tcctggagct cgagccgggc aagcgcatcc
     7621 tcgcgctcaa gaacgtgacc ttcaacgagc cgttcttccc cggccacttc ccgcagcacc
     7681 cggtgttccc gggcgtgctg gtcatcgagg cgatggcgca ggcgtcggcg atcctgacgt
     7741 tcaagacgct cggctccaag cccaggcccg actcggtcta ttacttcgtc ggcatcgacg
     7801 aggcgcgctt caagaaaccg gtgttcccgg gcgaccagct gctcctcgac gtctcgatcg
     7861 agcgcaacgt gcgcggcatc tggaagtaca cgggccaggc gcgcgtcggc gaccagctgg
     7921 tggccgaagg caggttgatg tgcacgaacc gggacctgtg atccacccga cggcggtcgt
     7981 gcacccgggc gcgacgctcg gaccccggac caccgtcggc gcctacagcc tcatcggcga
     8041 gcatgtcgag atcggggagg gcacgtcgat cggctcgcac gtcgtcatcg aggggcacac
     8101 gcgcatcggc gcgggcaacc gcatcttctc gcacagcgcg ctcggcggag cgccgcagga
     8161 caagaagtac gccggcgagc cgacgcggct cgagatcggc gaccgcaaca ccatccgcga
     8221 gttctgcacc ttcaacaccg gcaccgtgca ggacgcgggc gtcacgcgca tcggcagcga
     8281 caactggatc atggcctacg tccacatcgc gcacgactgc gacgtcggca gcaacaccat
     8341 cttcgccaac aacgcggcgc tcgccggcca cgtgcgcgtc ggcgactggg cgatcctcgg
     8401 cggctacacg atggtgcacc agttcgtctc gatcggcgcg cattcgatga cggccatcgc
     8461 gacggtcctg ctgctcgacc tgccgccgta cgtgatggcc gccgggaaca cggcgcagcc
     8521 gcacgggatc aacagcgagg gcctgaagcg gcgcaagttc tcccccgagt cgatcgcgcg
     8581 catcaaatcg gcctaccgca cgctgtaccg cgaggggctg aagctggagg aggcgcgcgc
     8641 cgagctgcag aagatggccg aggcgtcgcc cgagatcgcg ccgatggtcg agttcctcgc
     8701 cgcgtcccgg cgcggcatca cgcgctagat gatggccgcg ggagggcagc cgctcgtcgc
     8761 catggtcgcc ggcgaggcct ccggcgactt cctcggagcg cacctgatga cggcgctgcg
     8821 caagcgcttt cccgggctgc gcttcgccgg catcggcggg ccgaggatgg aaggcctcgg
     8881 cttcgactcc tggtatccga tggagcgcct cgcggtgcgg ggctacgccg aggtgctgcg
     8941 ccattacccg ggcctcgcga tgatgcggcg cgcgctcggg cgccggctga tcgcggcgcg
     9001 cccggcgctg ttcgtcggcg tcgacgcgcc ggacttcaac ctggggctcg agcgccgtct
     9061 caaggcggcc ggaatcccga cggcgcatta cgtcagcccc tcggtgtggg catggcgcgg
     9121 caagcgcgtc cggacgatcg cgcgcgcggt gagccggatg ctggtgctgt ttcccttcga
     9181 ggaggagatc taccgcgcgg ccggcgtccc cgtgacctac gtcggccatc cgctcgccga
     9241 gcagatcccg atggagtacg acgtcgagct cgcgcgcgag cagctcaagc tgcctccgac
     9301 acgcaggatc gtcgtcctga tgccgggcag ccggcagtcc gaggtgcgct acatggcccc
     9361 ggctttcgtg cgcgcggcga tgtgcatcgc cgagcagctc ccggcggcgc actttctcgt
     9421 gccgctggtg agccgcgaga cccgcgccat cttcgagcag gcgatgtacg atgccggcaa
     9481 tcccgagctc ccgctcacga tcctgttcgg gcacgcacgc gaggcgatcg cggccgcgca
     9541 cgccgtgctg gtcgcgagcg gcaccgcgac gctcgaggcg gcgctgtacc gcaagccgat
     9601 ggtgatcgcg tacaagatgg cgaagagcac gcaccgcatc atgtcgcgca tggcgtacca
     9661 gccctggatc gggctgccca acatcatcgc cggggagttc atcgtccccg agctgatcca
     9721 ggacgaagcg acgccggaga acctcgcgca ggcgctgctc aacgcgctcg ccgatcccgt
     9781 ggtcgcgagg cgcctgcccg agcggctcgc ccgcctgcac gagctgctgc ggcgcgactc
     9841 cgccgcgatc gtggccgaag cgctttcccc gtggctcgcc cgcgcctcgt ctgcggcata
     9901 gacgaggcgg gccgcggccc gctcgccggt cccgtctacg ccgcggcggt ggtcttcgac
     9961 ccgcgtcgcc gggtcccgcg gggcatccac gactccaagg tcctggcgag cggcgagcgc
    10021 gagcggatgg ccggcgtgat ccgcgaggcg gcgatcggct ggtgcgtggc gtatgcgacg
    10081 gtcgaggaga tcgaccggct gaacatcctg cgcgcgagcc tgctggcgat gcgtcgcgcg
    10141 gtgcaggggc ttccgctcat ccccgac
//
LOCUS       DATDMW010000319         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_965040, whole genome shotgun sequence.
ACCESSION   DATDMW010000319 DATDMW010000000
VERSION     DATDMW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965040"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VI319_13440"
     CDS             complement(<1..771)
                     /locus_tag="VI319_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864894.1"
                     /translation="MDELASGHAAARGDRGESAASAQDGRAARGGRLTARRVATLSWV
                     LCLVIALATLPLLAIGPGRVLPSDIFSGVGGVSFLVLAMTFASVGALVARRVPENKIG
                     WIFLLTGFANSVQLLSWQYADVGLHATDRLPGATAAAVFNTLISEATAGLLGLSLLLF
                     PGGRLPSRRWQPALVSFLVGMALLVLAGALRPGRYAEPFAKVSNPLGLAGARGVIEGV
                     DFAGWLFVIAGIGLGAAAMVVRLRRAGGIERQQLKIVLA"
     gene            938..1594
                     /locus_tag="VI319_13445"
     CDS             938..1594
                     /locus_tag="VI319_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864895.1"
                     /translation="MARHDTKSLRTAGITCIVGAAATTLGGIVVQGIVQPATTVSDHR
                     WSYPWSSGALVPVSILWASLHVLVFVGVLGFARSGVAGHSRSARLGTALALVGTALLF
                     VGEIASILTRDQRTDDTGAVIVGGVFALAILLTAVGFLAAGIATVRARLWQGWRRFTP
                     LTTGIVSFALLGLNVTKALPTGVAIYGLCLLALGVALHTQPTPESEERPVFAVQEQAA
                     "
     gene            1591..>2037
                     /locus_tag="VI319_13450"
     CDS             1591..>2037
                     /locus_tag="VI319_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864896.1"
                     /translation="MSEAAAPIELAPKRRSRRSGALRGFRVGGRWVHLILALAVVAGV
                     FVQVYLIGAYIFGAGQGALDAHKTAGWTVHGFEMLVFVAALAAWLPWVDTVLSLLLAV
                     IGTVQVALANEHRWIGGMHPVLALIVVGLATMIVLRALRRRRNARFP"
BASE COUNT          315 a          731 c          679 g          312 t
ORIGIN      
        1 ggcgagcacg atcttgagct gctgacgctc gatgcctccc gctcgtcgca ggcgcaccac
       61 catcgccgct gccccgagcc cgatcccggc gattacgaac agccagccag cgaagtcgac
      121 tccctcgatg actccgcgcg cgcccgcgag gcccaacggg ttcgacacct tcgcgaacgg
      181 ctcggcgtag cgcccgggac gcagcgcccc tgccagcacg agcagtgcca tcccgaccag
      241 gaagctcacg agcgccggct gccagcgccg cgacgggagc cgcccgcctg ggaaaaggag
      301 aagcgagaga ccgagcaatc ccgcagtcgc ctcgctgatc agggtgttga acacggccgc
      361 cgcggtcgcg ccaggcaggc ggtcggtcgc gtgcaggccg acatcggcgt attgccagct
      421 gagcagctgg acgctgttgg cgaagccggt gagcaggaag atccagccga tcttgttctc
      481 gggaacccgc cgagcgacga gcgcgccaac ggaggcgaac gtcatggcga gtacgaggaa
      541 cgagaccccg ccgaccccgg agaagatgtc gctcggaagc acgcgccccg gaccgattgc
      601 cagcaagggg agcgtcgcaa gggcgatcac gaggcagagg acccagctga gcgtcgcgac
      661 ccgacgcgct gtcagccggc cgccccttgc ggcccgcccg tcctgagccg atgcggcgct
      721 ctccccgcga tcgccacgcg ccgcggcatg ccccgacgca agctcgtcca cgccgacatt
      781 gtctcctccg ccgcgcgcct tggccagcgc gctggtactg ccgcgcaaat acgcactagc
      841 gctgaggtaa gtcacgcgct gccgcagatg acgcggcgac tccgtgttcc tagcgtggca
      901 gccaagagat cagcccaatg acacggagga gggcgacatg gcaagacacg acacgaagtc
      961 tctgcgcact gcaggtatca cctgcatcgt cggcgccgcg gcgaccacgc tcggcggcat
     1021 cgtcgtccag ggaatcgttc agccggcgac aaccgtctcc gatcacaggt ggagctatcc
     1081 ctggtcgagc ggcgcgctcg ttcccgtctc gatcctctgg gcgtccttgc acgtcctcgt
     1141 cttcgtcggt gtgctcgggt tcgcgcggag tggcgttgca ggccacagtc gcagcgcgcg
     1201 cctcggcacc gcgctcgcgc tcgttggcac cgcgctgctc ttcgtcggcg agatcgcgtc
     1261 aatcctgact cgcgatcagc gcaccgacga caccggcgca gtgatcgtgg gcggggtctt
     1321 cgccctcgcc atcctgctaa cggcggttgg cttcctcgcg gccggaatcg cgaccgtgcg
     1381 cgcacggctc tggcagggct ggcgccgctt cacgccgctg accaccggca tcgtgtcctt
     1441 tgcgctcctc ggcctgaacg tcaccaaggc tctcccgacc ggtgtcgcga tctacggcct
     1501 ctgcctgctc gcgcttggag tcgcgctcca cacgcagccg acgcccgagt ccgaggaaag
     1561 gccggtgttc gccgtgcagg agcaagcagc atgagcgagg cggccgcccc gatcgagctg
     1621 gcgccgaagc ggcggtcgcg cagatccgga gcgctccgcg ggttccgggt cggcgggcgg
     1681 tgggtgcacc taatcctcgc gctcgcggtc gtcgccggcg tgttcgtgca ggtctacctg
     1741 atcggcgcct acatcttcgg cgcaggccag ggcgcactcg acgcccacaa gacggcgggc
     1801 tggacggtcc acggcttcga gatgctggtg ttcgtcgccg cgctcgccgc ctggcttccg
     1861 tgggtcgata ccgtcctgtc gttgctgctc gcggtgatcg gaacggtgca ggtagctctc
     1921 gcaaacgagc accgttggat cggcggaatg cacccggttc tcgcgctcat tgtggttggc
     1981 ctcgcgacaa tgattgtcct gcgcgcactc cgacgacgcc gcaacgcgcg cttcccg
//
LOCUS       DATDMW010000320         2446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_965177, whole genome shotgun sequence.
ACCESSION   DATDMW010000320 DATDMW010000000
VERSION     DATDMW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965177"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VI319_13455"
     CDS             <1..1083
                     /locus_tag="VI319_13455"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864897.1"
                     /translation="VGYLSADFRDHVMGRLMLDVIGRHDPARVSVHAYSLAPREVEDA
                     MTERFRSACAGFRRLDDLDHRGAARAIADDELDVLIDLMAHSGSSRPGILLYKPAPVI
                     ATHLGAHGAIGLRQVDFKLTDAEVDLPDAAQYQIETPLLLEGCVLPVRRVPPAAAPPT
                     TREALGLAPETVVFGTFVSLAKLSPRCLRLWRTILDRVPGSVLAFSPHRDEARALFVR
                     RLESFGIDRARIVFVPWTMDEAADRARYTLVDVVLDTLPYTGGDTTAAALDMAVPVVT
                     RVGERAAERMTWSLLAHLGVTDTAAKSDEEYVGVACRLAQDRGWRARISAVIASRLPA
                     SGLADLDRYARSLETAYERAVALKGR"
     gene            1088..>2446
                     /gene="mpl"
                     /locus_tag="VI319_13460"
     CDS             1088..>2446
                     /gene="mpl"
                     /locus_tag="VI319_13460"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY6864898.1"
                     /translation="MHLHVLGICGTFMGGLAALARSAGHTVTGCDANVYPPMSTQLAA
                     LGIALTEGYDAGQLDGVARSADVFVVGNAISRGNALLEAILDAGRPYTSGPQWLAENV
                     LRDKWVLAVAGTHGKTTTSALLAWILDRAGLAPGFLIGGVPVDFAVSARLTSAPFFVI
                     EADEYDTAFCDKRSKFVHYRPRTAILNNLEYDHADIFPDLAAIETQFHHFVRTIPRNG
                     RLVVNAADAALERVLSRGLWSEMERFAAEEANATADWTISEAGGIRHAGVAEDRLASF
                     SLLGAHNRLNALAAIAAASHASVPVQQSIAALASFRGVKRRLEIRGTAAGITVYDDFA
                     HHPTAIAATVGALRREVGDARILAVLEPRSNTMKLGTMKAALPGSLIEADLVFCYAAR
                     LGWDPAAALSALGARARTSSDLSALIDAIAGAARPGDHVLVMSNGGFGGIHDKLLARL
                     GER"
BASE COUNT          340 a          872 c          853 g          381 t
ORIGIN      
        1 gtgggctatc tctcggcgga tttccgcgac cacgtcatgg gccggctcat gctcgacgtg
       61 atcgggcgcc acgatccggc gcgcgtatcg gtgcacgcgt actcgctggc gccgcgggag
      121 gtcgaggacg cgatgacgga gcgttttcgg agcgcctgcg caggcttccg cagactcgac
      181 gatctcgatc atcggggcgc tgcgcgcgcg atcgcggacg acgagctcga cgtcctgatc
      241 gacctgatgg cgcactccgg ctcctccagg cccgggatcc tgctctacaa gccggcccct
      301 gtgatcgcga cgcatctcgg cgcgcacggc gcgatcggcc tgcgtcaggt ggacttcaag
      361 ctcaccgacg cggaagtcga tctcccggac gcggcgcagt atcagattga gacgccgctc
      421 ctgctcgaag gctgcgtgct gccggtccgg cgcgtacccc ccgcggccgc gccgccgacg
      481 acgcgcgagg cgctcgggct cgcgccggag accgtcgtct tcgggacgtt cgtgagcctc
      541 gccaagctct cgccgcgctg ccttcgcctc tggcgcacga tcctcgaccg cgtcccgggc
      601 agcgtgctcg cgttttcgcc gcaccgggac gaagcgcgcg cgctcttcgt gcggcggctc
      661 gaaagcttcg gcatcgatcg cgcccggatc gtattcgttc cctggacgat ggacgaggcc
      721 gccgatcgcg cgcgctacac gctcgtcgac gtcgtgctcg acacgctgcc gtacacgggc
      781 ggcgacacga ccgcggcggc gctcgacatg gcggtgccgg tggtcactcg cgtgggcgag
      841 cgcgccgccg agcggatgac ctggagcctg ctcgcgcacc tcggcgtaac cgacaccgcg
      901 gcgaagagcg acgaggagta cgtcggggtc gcctgccgcc tcgcgcagga tcgcggctgg
      961 cgagcgcgca tctcggctgt gatcgcttcg aggctcccgg cttccggcct cgccgacctc
     1021 gaccgctacg cgcggtcgct cgagaccgcg tacgagcgcg cagtggcgct gaagggtcgc
     1081 tgaggcgatg catctgcacg ttcttggcat ctgcgggacc ttcatgggcg gtctggccgc
     1141 gctcgcgcgc tcggccggcc ataccgtgac cggctgcgac gccaacgtct atccgccgat
     1201 gagcacgcag ctcgccgcgc ttggaatcgc gctcaccgaa ggctacgacg cggggcagct
     1261 cgacggcgtc gcgcggagcg cggacgtctt cgtcgtcggc aacgcgatct cgcgcggcaa
     1321 tgcgctgctc gaggcgattc tcgacgccgg tcggccgtac acctccgggc cacaatggct
     1381 cgccgaaaac gtgctgcgcg acaaatgggt gctggcggtg gccggcacgc acggcaagac
     1441 cacgaccagc gcgctgctcg cgtggatcct cgatcgcgcc ggactcgctc cgggattcct
     1501 gatcggcggc gtgcccgtgg acttcgccgt ctcggcccgg ctcacgagcg cgccgttctt
     1561 cgtcatcgag gccgacgagt acgacaccgc gttctgcgac aagcgctcca agttcgtcca
     1621 ttatcggccg cgcaccgcca tcctgaacaa cctcgagtac gatcacgccg acatctttcc
     1681 cgatctggcc gcgatcgaga cgcagttcca tcatttcgtg cgcacgatcc cgcggaacgg
     1741 acggctcgtc gtcaacgcag ccgacgcggc gctcgagcgc gtgctctcgc gcgggctgtg
     1801 gagcgagatg gagcgcttcg ccgccgagga ggcgaacgcg accgccgact ggacgatctc
     1861 tgaggcaggc ggcattcgcc acgcgggcgt cgcggaggac aggctcgcgt ccttttcgct
     1921 gctcggcgcg cacaaccggc tgaacgcgct ggccgcgatc gccgccgcga gtcacgcgtc
     1981 cgttccggtg cagcagagta tcgcggcgct cgcgagcttc cgcggcgtca agcggcgcct
     2041 ggagatccgg ggaaccgccg ccgggatcac ggtctacgac gacttcgcgc accatcccac
     2101 ggcgatcgcg gcgaccgtcg gggcgttgcg ccgcgaagtc ggcgacgcgc gcattctggc
     2161 ggtgctcgag ccgcgctcca acacgatgaa gctgggcacc atgaaggcgg cgctgcccgg
     2221 aagcctcatc gaggcggatc tggtgttctg ctacgccgcg cgcctgggat gggatccggc
     2281 ggccgcattg tctgcgctgg gcgcgcgggc gcgcacctcg agcgatcttt cggcgctgat
     2341 cgacgcgatc gcaggcgccg cgcggccggg cgaccacgtt ctcgtcatga gcaacggcgg
     2401 gttcggcggc atccacgaca agctgctcgc gcgcctcggg gagcgg
//
LOCUS       DATDMW010000321         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1091461, whole genome shotgun sequence.
ACCESSION   DATDMW010000321 DATDMW010000000
VERSION     DATDMW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091461"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VI319_13465"
     CDS             complement(<1..350)
                     /locus_tag="VI319_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864899.1"
                     /translation="MTVKRMDNVGILVDSLDAAISFFAELAPGPLDDVRSIDGDAPAK
                     QVDGSLDAQEQRPALSLELDRIGIDWPPMIGPRAQRQRRQLGGEGRTALAEPLEVQAR
                     ASGTDEVGPFVDTA"
     gene            487..>2001
                     /locus_tag="VI319_13470"
     CDS             487..>2001
                     /locus_tag="VI319_13470"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6864900.1"
                     /translation="MNRVFERRSWSGAILLAAALSLAPMALHAEDRDDEDGNAPDLVL
                     LNGKISTVDALNSTVEAIAIRRGEIIATGRSGRVRGLARRNTEVIDLHGRRVLPGLID
                     GHLHGLRNGYHCFTQTVRLDNVFTRAEALAKYAAKGRQLPADTWIFTTAGWTVAQLDS
                     PGMFTLAELDAALPSNPVFIVASGAAGAQVNTRALQLLGLDPGTPPGRLTGARQTAAG
                     AAVIAQFDKLSIDEQANCLVDFIRDANSNGLTAWNDPEGNQQPFNTAGSCLEFGVGQH
                     DHQAVIQLRRDDRLNARIVFKVMNNFSGLTQLVSDQRHPLAFIGDEMLRFLGVGEEVL
                     CPGDQPAPDAAQYQAIANFLASNRMTFENHASTDATQRAVLNAWEQANTIYPISKLHW
                     SIAHPGNDGVSPTDATLARVKALGIGMTPSSSGALGSGRTPRFKSMYDSGIHLCISTD
                     AMNVAPYPPFVDLWYVISGNSLNPAVPGVPADQRLTRGEALRARTTNCAWNLLQE"
BASE COUNT          364 a          671 c          642 g          324 t
ORIGIN      
        1 cttgcggtgt cgacgaaggg gccgacttcg tcggtcccgg aggcccgggc ctggacttcc
       61 agcggttcag caagagctgt ccggccttca ccgccgagct gtcggcgctg gcgctgcgca
      121 cgcgggccaa tcattggggg ccaatcaata ccaatacggt cgagctcaag cgagagtgct
      181 ggtcgctgct cctgggcatc gagggagcca tcgacctgct tggcgggtgc gtcgccgtcg
      241 attgatcgaa cgtcgtccag aggccccggg gccagctcgg cgaagaaaga gatggcggca
      301 tcgagcgagt ccaccaggat gccgacgttg tccatccgct tgaccgtcat cgacgttctg
      361 cctttcgata aggtcaaggg tgttcggggg taaacacgag cgttcggacc cctccgcgcg
      421 gagggaagct cgccttgacc tcattttggg cgggtgccta tagccgcacc atccaggagg
      481 gatcaaatga atcgcgtctt cgagaggcga agctggtccg gtgcaatcct cctcgcggct
      541 gccctcagcc ttgccccgat ggctctccat gctgaggatc gcgacgacga ggacggcaac
      601 gctcccgatc tcgtgctcct caacggaaag atttccaccg tcgacgcctt gaactcgacc
      661 gtcgaggcga tcgcgatccg ccgcggcgag atcatcgcca cgggcagaag cggcagggta
      721 cgcggactcg ccaggcgcaa cacggaggtg atcgacctcc acggccgccg cgtgcttccc
      781 ggcctcatcg acggccacct ccatggtctg cgcaacggct accactgttt cacgcagacg
      841 gtgcgcctcg acaacgtgtt tacgcgggcc gaggcgctcg cgaagtatgc ggccaagggc
      901 aggcagcttc cggccgacac ctggattttc acgacggcag gatggacggt cgcgcagctt
      961 gattccccgg gaatgttcac gcttgccgag ctcgatgcgg cgctcccgtc gaatcccgtg
     1021 ttcatcgtcg cgtccggagc ggccggcgca caggtgaaca cgcgcgcact ccagttgctg
     1081 ggcctcgatc cgggaacccc gccgggacgg ctgaccggcg cacgccagac cgccgcgggc
     1141 gccgcggtca tcgcgcaatt cgacaagctg tcgatcgacg agcaggcgaa ctgcctcgtc
     1201 gacttcatcc gcgatgccaa cagcaacggc ctgacggcct ggaacgatcc cgagggaaac
     1261 cagcagccct tcaacacagc gggcagctgc ctcgagttcg gcgtcggcca gcacgaccat
     1321 caggcagtga tccagctgcg ccgggacgat cggctgaacg cgcgcatcgt cttcaaggtg
     1381 atgaacaact tctccgggct gacccagctc gtttcggacc aacggcatcc gctcgctttc
     1441 atcggcgacg agatgcttcg cttcctcggc gtgggcgagg aagtgctctg tccaggagac
     1501 cagcctgctc cggacgccgc gcagtaccag gcgatcgcga acttcctggc ctcgaaccgg
     1561 atgaccttcg agaaccacgc cagcacggat gcgacgcaga gggccgtgct caacgcgtgg
     1621 gagcaggcga acacgatcta cccgatctcg aagctgcact ggtccattgc ccaccccggc
     1681 aacgacggcg tgagtccgac cgatgcgact ctggcgcgcg tcaaggctct cggcatcggc
     1741 atgaccccga gcagcagcgg cgcgctgggc agcggccgca cgccgcggtt caagagcatg
     1801 tacgacagcg gcatccacct ctgcatctcg acggacgcga tgaacgtcgc gccctacccg
     1861 ccgttcgtgg acctctggta cgtgatcagc ggcaactcgc tcaatccagc ggtgcccggg
     1921 gttccagccg atcagcgctt gacgcgaggg gaggcacttc gagcgaggac cacgaactgc
     1981 gcatggaacc tgctgcagga g
//
LOCUS       DATDMW010000322         3631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2086469, whole genome shotgun sequence.
ACCESSION   DATDMW010000322 DATDMW010000000
VERSION     DATDMW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086469"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /gene="ftsH"
                     /locus_tag="VI319_13475"
     CDS             <1..1266
                     /gene="ftsH"
                     /locus_tag="VI319_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY6864901.1"
                     /translation="IPKGVLLVGPPGTGKTLLARAVAGEAKVPFFSLSGSEFVEMFVG
                     VGAARVRDLFEQARLKAPAIIFIDELDALGRARGAYGVGGHDEKEQTLNQLLVELDGF
                     DASKGLVVLAATNRPEILDPALLRAGRFDRQVLVDRPDKKGRSQILQVHLKKVRLAAD
                     ADVEKIAALTPGFTGADLANLVNEAALLATRRGADAVALGDFTAAVERIVAGLEKKNR
                     LLNPFERRVIAYHEMGHALIAMSLHGADPVHKVSIIPRGVGALGYTIQRPTEDRFLMT
                     RGELENKMAVLLGGRAAERLVFDHLSTGAADDLAKVTDIARGMVMRYGMDPALGNVTY
                     EGERPAFLEQGPGAVTPERRFSEDTAREIDRAVREIVDAAFSRALGILKERRRTLEDG
                     AQALLAKETLVEEDLMRLVGAAPRSEVTA"
     gene            complement(1256..2080)
                     /locus_tag="VI319_13480"
     CDS             complement(1256..2080)
                     /locus_tag="VI319_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEY6864902.1"
                     /translation="MSIVYGSDAFSPRQIAEKIEDGGVAKARLPLLSMAMLGVLAGGF
                     IGLGGITSTLVLSDPGLGFAAGRLLAGLTFSLGLVLVVVAGAELFTGNNLLVMAWADR
                     RITTAEMLRNWAVVYLANAVGAVGLALIVWLANHGAMNGGAVALQYAKLAAAKASLPF
                     AEAFFKGVLCNILVCLAVWIAMAGRSVADKVLAIVLPISAFVAAGFEHSVANMFLIPA
                     GILMGHDAHVAVPDWAGLARNLVPVTLGNVFGGSVLVALVYFVIYRRGAEKQAAKR"
     gene            2186..>3631
                     /locus_tag="VI319_13485"
     CDS             2186..>3631
                     /locus_tag="VI319_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160136.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY6864903.1"
                     /translation="MTDLSATRRAAPYRARNRIRFVTAASLFDGHDATINIVRRILQA
                     SGCEVVHLGHNRSVEDIVTAALHEDVHGIAVSSYQGGHLEFYKYMIDLLRERGGGDIK
                     VFGGGGGVIVAEEARALEAYGVARVYSPEDGQRMGLQGMIDDMLERCDADLSKGAPPD
                     ADAVKRGDRRALARLITVLESGATGALRDRLHAEAKQRPAPVLGVTGTGGSGKSSLVD
                     ELIRRFRLDQDDRLQIAVLAVDPSRRKSGGALLGDRIRMNAIGPWHGRARVFMRSLAT
                     REAGREISAALGDAIAACRVAGFDLIIAETSGIGQGDSAIVPLADASLYVMTPEFGAA
                     SQLEKIEMLDFADFAAINKFDRRGAADALRDVRKQLQRNRGLFATPAEEMPVFGTIAS
                     RFNDDGVTALYQAIRERLVEHGLQARDGRLRGAGVRSSSGASAIVPPARVRYLAEIAE
                     TVRGYHARSAAQAKLARERQQLAESKRMLGAD"
BASE COUNT          555 a         1306 c         1291 g          479 t
ORIGIN      
        1 attccaaaag gtgttctgct ggtcggtcct ccgggcaccg gcaagaccct cctggcccgg
       61 gcggtggcgg gcgaagcaaa ggttccgttc ttcagcttga gtggctccga gttcgtcgaa
      121 atgttcgtcg gcgtcggcgc cgcgcgcgtg cgggacctct tcgagcaggc gcggctgaag
      181 gcaccggcga tcatcttcat cgacgagctc gacgcgctcg gacgcgcgcg gggcgcctac
      241 ggcgtcggcg gccacgacga gaaggagcag acgctcaacc agctgctggt cgagctcgac
      301 ggcttcgacg cgagcaaggg gctggtcgtg ctcgccgcca ccaaccgccc cgagatcctc
      361 gacccggcgc tgctgcgcgc gggacgcttc gaccgccagg tgctcgtcga ccgtcccgac
      421 aagaagggcc gctcgcagat cctgcaggtg cacctgaaga aggtgcggct cgcggccgac
      481 gccgacgtcg agaagatcgc ggcgctcacg ccgggattca ccggcgccga tctcgcgaac
      541 ctcgtcaacg aggcggcgct gctcgcgacg cgacgcggcg ccgacgcggt cgcgctcggc
      601 gacttcaccg ccgcggtcga gcgcatcgtc gccggcctgg agaagaagaa ccgattgctc
      661 aaccccttcg agcgccgcgt gatcgcctac cacgagatgg gccatgcgct gatcgcgatg
      721 tcgctgcacg gcgccgaccc cgtgcacaag gtgtcgatca tcccgcgcgg cgtcggcgcg
      781 ctcggctaca cgatccagcg gccgaccgag gaccgcttcc tcatgacgcg cggggagctc
      841 gagaacaaga tggccgtgct cctcggcggg cgcgccgccg agcgcctcgt cttcgaccat
      901 ctgtccaccg gcgcggccga cgacctcgcc aaggtgaccg acatcgcgcg cggcatggtg
      961 atgcgctacg gcatggaccc ggcgctcggc aacgtgacct acgagggcga gcgcccggcc
     1021 ttcctcgagc agggcccggg cgcggtgacg cccgagcggc gcttctccga ggacacggcg
     1081 cgcgagatcg accgcgccgt gcgcgagatc gtcgacgcgg cgttctcgcg cgcgctcggc
     1141 atcctcaagg agcggcggcg cacgctcgag gacggcgcgc aggcgctgct cgcgaaggag
     1201 acgctggtcg aggaggacct gatgcgcctg gtcggcgccg cgccgcgcag cgaggttacc
     1261 gcttagcggc ctgcttctcc gcgcctcggc ggtagatgac gaagtagacc agcgcgacga
     1321 gcacgctgcc gccgaagaca ttgccgagcg tgaccggcac gaggttgcgc gcgagcccgg
     1381 cccagtcggg taccgcgacg tgcgcgtcgt ggcccatcag gatgccggcc gggatcagga
     1441 acatgttggc cacgctgtgc tcgaacccgg cggcgacgaa ggccgagatc gggagcacga
     1501 tggcgagcac cttgtcggcg acgctgcggc ccgccatcgc gatccagacg gcgaggcaca
     1561 ccaggatgtt gcacagcacg cccttgaaga aggcctcggc gaagggcagc gacgccttgg
     1621 cggccgcgag cttcgcgtac tgcagcgcca ccgcgccgcc gttcatggca ccgtgattcg
     1681 cgagccatac gatcagcgcg aggccgaccg ccccgacggc gttggcgagg tagacgaccg
     1741 cccagttgcg cagcatctcg gccgtcgtga tgcgccggtc cgcccaggcc atcaccagca
     1801 ggttgttgcc ggtgaagagc tccgcgcccg cgaccacgac gaggaccagc ccgagcgaga
     1861 acgtcagccc cgcgagaagg cgcccggccg cgaacccgag gccggggtcg gagagcacca
     1921 gcgtgctcgt gatgccgccc agcccgatga agccgcccgc gagcacgccc agcatcgcca
     1981 tggacagcag cggcaggcgc gccttggcga cgccgccgtc ctcgatcttc tcggcgatct
     2041 ggcgcggcga gaaggcgtcc gaaccgtata cgatgctcat gcccggggct cctttcgccg
     2101 ctacactagc gccaaaggcg ccggggcgcc gtgattgacg ttaacgttaa cgtaaagtag
     2161 cctcagctgc catgacggac gtcccatgac ggacctgagc gccacccgcc gcgccgcgcc
     2221 gtaccgggcg cgcaaccgca tccggttcgt gaccgcggcc tccctgttcg acggccacga
     2281 cgcgaccatc aacatcgtgc gccgcatcct gcaggcgagc ggctgcgagg tcgtccatct
     2341 cggccacaac cgctcggtcg aggacatcgt caccgcggcg ctgcacgagg acgtgcacgg
     2401 catcgccgtc agctcgtacc agggcggcca cctcgagttc tacaaataca tgatcgacct
     2461 gctgcgcgag cgcggcggcg gcgacatcaa ggtgttcggc ggcggcggcg gcgtgatcgt
     2521 cgccgaggag gcgcgcgcgc tcgaggccta cggcgtcgcg cgcgtgtact cgcccgagga
     2581 cggccagcgc atgggcctgc agggcatgat cgacgacatg ctcgagcgtt gcgacgccga
     2641 tctctcgaag ggcgcgccgc cggacgccga cgcggtgaag cgcggtgacc gccgcgcgct
     2701 cgcgcgtctg atcacggtcc tcgagagcgg cgcgaccggc gcgctgcgcg accggctgca
     2761 cgcggaggcg aagcagcgtc cggcgcccgt cctgggcgtc accggtacgg gcggctcggg
     2821 caagtcgtcg ctcgtcgacg agctgatccg gcgcttccgc ctcgaccagg acgaccggct
     2881 gcagatcgcg gtgctcgcgg tcgatccttc ccggcgcaag tcgggcggcg cgctgctcgg
     2941 cgatcgcatc cgcatgaacg cgatcgggcc ctggcacggg cgcgcccgcg tgttcatgcg
     3001 ctcgctcgcg acgcgcgagg cgggccgcga gatctcggcc gcgctcggcg acgcgatcgc
     3061 cgcgtgccgc gtcgcgggct tcgatctgat catcgccgag acctcgggca tcggccaggg
     3121 cgactcggcc atcgtgccgc tcgccgacgc ctcgctctac gtgatgacgc ccgagttcgg
     3181 cgcggcgagc cagctcgaga agatcgagat gctcgatttc gccgacttcg cggcgatcaa
     3241 caaattcgac cgccgcggcg cggccgacgc gctgcgcgac gtgaggaagc agctgcagcg
     3301 caaccgcggc ctgttcgcga cgcccgcgga ggagatgccg gtcttcggca ccatcgcctc
     3361 gcgcttcaac gacgacggcg tcacggcgct gtaccaggcg atccgcgagc ggctggtcga
     3421 gcacgggctg caggcgcgcg acgggcgcct gcgcggcgcc ggcgtgcgct cgtcctcggg
     3481 cgcgagcgcg atcgtgcccc cggcgcgcgt gcgctacctc gccgagatcg ccgagaccgt
     3541 gcgcggctac cacgcgcgct ccgcggccca ggcgaagctc gcgcgcgagc gccagcagct
     3601 cgcggagtcc aagcgcatgc tgggcgcgga c
//
LOCUS       DATDMW010000323        12331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1589459, whole genome shotgun sequence.
ACCESSION   DATDMW010000323 DATDMW010000000
VERSION     DATDMW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12331
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589459"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /gene="gatB"
                     /locus_tag="VI319_13490"
     CDS             complement(<1..835)
                     /gene="gatB"
                     /locus_tag="VI319_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY6864904.1"
                     /translation="MTTSAWEIVVGLETHAQLSTRSKMFSGASTAFGAPPNTQASAVD
                     IALPGVLPVANRGAVERAIRFGLAVGGRDTINRRSIFARKNYFYPDLPKGYQISQYEI
                     PIVQGEPVSNATINFLVKEGIKHVRLTRAHLEEDAGKSLHEDFHGMTGIDLNRAGTPL
                     LEIVSEPDMRSAEEAVSYARALHGLVRWIGICDGNMQEGSFRCDANVSVRRKGDPNLG
                     TRCEIKNLNSFRFLERAIEYEAKRQIEILEDGGKIVQETRLYDPERDETRSMRTKEEA
                     HD"
     gene            complement(832..2313)
                     /gene="gatA"
                     /locus_tag="VI319_13495"
     CDS             complement(832..2313)
                     /gene="gatA"
                     /locus_tag="VI319_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY6864905.1"
                     /translation="MHDRSLKQLSAALARREISSVDLTRLFLGRAQKSQAGLNAFISL
                     DPERSLAQARAADERIAAGAGTALTGIPIAHKDIFLARGWPATCGSRMLADFIAPYDA
                     HVIERFDAAGCVVLGKTNMDEFAMGSSNENSHFGPVKNPWDGARVPGGSSGGSAAAVA
                     ARIAPAATGTDTGGSIRQPAALSGICGLKPTYGVVSRYGMVAYASSLDQGGPMAKTAE
                     DLALLMNVMAGYDARDSTSLERPAEDFARELERPLAGGATKPLGGLRVGVCRDHFGAG
                     LAGEVGTAVEASIAALEKLGARRVEIELPNNRLSLAAYYVIAPAEASSNLSRYDGVRY
                     GYRAKEYADLLDMYRKTRAQGFGAEVKRRILVGTYVLSHGYYDAYYLKAQKVRRLIAR
                     DYAEAFRACDVIAGPTTPGVAFAIGAKASDPVQMYLSDIYTVPVNLAGLPALSIPCGT
                     GEGGLPVGLHLVGDYFSEARLLGIAHRYQQASDWHLRAPKAAA"
     gene            complement(2338..2625)
                     /gene="gatC"
                     /locus_tag="VI319_13500"
     CDS             complement(2338..2625)
                     /gene="gatC"
                     /locus_tag="VI319_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY6864906.1"
                     /translation="MRLSPEEVQRIAMLARLEIGAAETQALQSQLNDILSMIGEMQKV
                     NTDGIEPMSHARDVTLRLREDNVTELDRREPYQAVAPRVDSGLYLVPKVIE"
     gene            2781..3824
                     /locus_tag="VI319_13505"
     CDS             2781..3824
                     /locus_tag="VI319_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY6864907.1"
                     /translation="MFGFLRGYFSDDMAIDLGTANTLIYVRGKGIVLDEPSVVAIRQE
                     GGPNGKKTIQAVGKEAKQMLGKTPGNITAIRPMKDGVIADFTVTEQMLKQFIKKVHES
                     RLFSPSPRIIICVPCGSTQVERRAIREAATEAGASQVYLIEEPMAAAIGADLPVAEAT
                     GSMVVDIGGGTTEVGVISLGGMVFSGSVRVGGDKFDEAIINYIRRNYGMLIGETTAET
                     IKKEIGSAFPGSEVREMEVKGRNLAEGIPRSFTISSNEILEALTEPLNQIVGAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDLDRLLMEETGLPVIVADDPLTCVVRGSGKAL
                     EKMEKLGSIFANE"
     gene            3923..4834
                     /gene="mreC"
                     /locus_tag="VI319_13510"
     CDS             3923..4834
                     /gene="mreC"
                     /locus_tag="VI319_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY6864908.1"
                     /translation="MDYQPPPFFSRGPAPLVRLMFFISLAVLLMVLDARFRYTESLRQ
                     VVAVVVYPLQRLALLPVDLANGAADYFTSLVTLKHENEQLKTRQLESANDLLTLESLR
                     AENVQLRRMLDAQEQLPRKATFAEILYMSRDPFSRKVVIDKGSQQGLQTGQAVIDDVG
                     VIGQVTRVYPLLSEVTLVTDREQAIPAQIVRNGLRAVVYGGSDPGTLELRYMPTNADI
                     QNGDVLVTSGIDGTYPEGLPVARVVKIERDAAYAFAKVSCVATTGPDRHRQVLVLAYD
                     WKQPPPPPQAADQAPKPAAKAKRPKRP"
     gene            4847..5368
                     /gene="mreD"
                     /locus_tag="VI319_13515"
     CDS             4847..5368
                     /gene="mreD"
                     /locus_tag="VI319_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY6864909.1"
                     /translation="MAFDPRFQGIPRPVNVGFMALTAAAAMLFNMLPWRDVIGLPDLL
                     ALVIAFWCVHEPRKMGIGFAWALGLLMDAGNGALMGQHAFAYAILAFGAIALHRRILW
                     FSPWAQAAHVLILLVASQLLMLLVRMAAGGAYPGLGYFIGPVIAAALWPVATYVLLLP
                     QRRPRHSDEPRSI"
     gene            5365..7275
                     /gene="mrdA"
                     /locus_tag="VI319_13520"
     CDS             5365..7275
                     /gene="mrdA"
                     /locus_tag="VI319_13520"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY6864910.1"
                     /translation="MRFAELKNTQRELHYFQLRVGFAGMAVVIAFGLLFARFFYLQVI
                     QHDYYHTKAEDNRISIVPVLPNRGLIVDRNDVVLARNYAAYTLEISPSKVANLKATID
                     ELAGIVDIQPKDRRRFQKMMEESKTLDSIPIRIRLTDEEVAKITVLKYKLPGVDVKAR
                     LFRQYPLAELASHVTGYINRITDRDLERIEEAGLDANYKGTDHIGKTGLEQSYEQELH
                     GTTGFEQVEIDAGGRGVRTLSRTAPISGNNLTLTLDIRLQQVADEAFGDRRGALVAIE
                     PSTGGILAFVSKPGFDPNLFVDGIDPANWDELNNSPDLPLNNRALAGLYPPGSTFKPY
                     MALTALEVGKRTPTQSIFDPGYFNFGGHRFRDDKAGGHGYVDMYKSIVESCDTYYYIL
                     GNDLGIDTMARFIGQFGFGQRTGVDVEGEAIGVLPSQEWKLKRFRRPEQQKWYAGETI
                     SVAIGQGYNSYTPLQLAQAVATLANNGVMYKPHFVNYIESAKTGERTYVEPEPVKTIK
                     LKPQNIQFIKRAMVGVNKEGTGAVAFRGAEYTSGGKTGTAQVIAIKQTEKYDEKRTAE
                     RHRDHALFIAFAPVENPKIALAVVVENAGFGARAAAPIARQVLDYYLLGKRKGKPAET
                     AVTPQSDDDESD"
     gene            7262..8362
                     /gene="rodA"
                     /locus_tag="VI319_13525"
     CDS             7262..8362
                     /gene="rodA"
                     /locus_tag="VI319_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY6864911.1"
                     /translation="MRAIDNLVRRITGRLDGPLLGIATALLALGLLTLYSASNENPAR
                     VSAQVANILVAFALMWVIAQVSPQTLMRFALPVYMLGLLLLVAVALFGDVRNGARRWL
                     SFGVGSVQPSELMKIAMPLMLAWYFHRHETALRFRDYLVAMILLAVPVALIVRQPDLG
                     TAMLVTAAGFFVIFLAGLSWKIIVGGGVAILGAMPLLWGMLHDYQRKRVLTLLDPSQD
                     PLGAGYHIIQATIAVGSGGLVGKGWLNGTQTHLEFIPERSTDFIFAVFSEEFGLLGNG
                     LLLVLYLSLVTRGLMIAANAPTFFARLLAGSLTLTLFTYAFVNMGMVSGLLPVVGVPL
                     PFISYGGTALVTLFIAIGMLMSIQNHRTLVQT"
     gene            8359..9267
                     /locus_tag="VI319_13530"
     CDS             8359..9267
                     /locus_tag="VI319_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY6864912.1"
                     /translation="MSLNADAARDRGVRGALVAIAVSILLAACGTSPKRPGGYYKDDG
                     PGASPPKNVESTPDAAPRVEPLHKSANNPYTVFGRHYVPAKTLRPYKERGIASWYGKK
                     FHGQMTSSGERYDMYKMTAAHTTLPIPSYARVTNLKNGRSVIVRINDRGPFHSGRIID
                     LSYAAAAKLGYVNAGSAPVEVEALLPGEMSRAAEEQPAASAAPVEAPPAVAAEALPAE
                     AIPAAPDAKGVYLQLGAFSARDNADALRGRLERELSWVDRSFEVLPVDGLFRVRLGPY
                     RDRGEADGVALRIREALDLKPVVIER"
     gene            9307..10440
                     /locus_tag="VI319_13535"
     CDS             9307..10440
                     /locus_tag="VI319_13535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY6864913.1"
                     /translation="MSRFPLARFVLLFASLCGSAAAQAPQPPAIAARAWVLMDVSSGQ
                     PITARNADERMEPASLTKLMTAYVAFAAIRQKTVAIDQAVPVSVRAWRVGGSRMFIQP
                     NKKVTVEELLYGMIVDSGNDACIALAEAIAGSEEAFVQMMNREAQRLGMSNTNFTNVS
                     GLPDPKHYSTANDLALLTGALIRDFPEYYKLYSVREYRYNNITQLNRNRLLWLDPNVD
                     GVKTGHTESAGYCLVASAQRGSRRLMSVVLGTSSETLRAQESQKLLNYGFQFFDSVRL
                     YQKEHPISTLEVWKGSVSRLHAGVLRDLFVTVPKGDATRLKAELLSEQPLIAPIAQGQ
                     KVGTLHVTLDGKPLADYPVVALEAVGIAGWLGRSWDSLRLMFK"
     gene            10454..11323
                     /locus_tag="VI319_13540"
     CDS             10454..11323
                     /locus_tag="VI319_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEY6864914.1"
                     /translation="MIYLNGDFMPLEQAKIPVLDRGFIFGDGVYEVIPVYSRRPFRLP
                     GHLARLQASLDAISIENPHSGEKWRELIERIVAENPWEDQAVYLQVTRGVARRDHTFV
                     RGVAPTVFIMSNPLVTPSAEVVKNGGAAIVRPDFRWLRCDIKTTSLLGNCMLRTEAAD
                     AGCTESILVRDGFLTEASASNVFVVREGTVLAPPKSHLMLPGITYDVVLELLAANGVP
                     HAARPVAEAELRAADEIWVTSSSREVMAITTLDGKPVGRGEHAGRPGPVFARVYALYQ
                     QYKAKVMRAPVHA"
     gene            11316..11585
                     /locus_tag="VI319_13545"
     CDS             11316..11585
                     /locus_tag="VI319_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEY6864915.1"
                     /translation="MPDPEAPLLAFPCEFPIKIMGLTQKGFAQAVIEVVARHAPDFDP
                     ATMEMRPSREGKYLSLTCTIRAVSREQLDALYRELCDHPMVTMVL"
     gene            11639..12247
                     /gene="lipB"
                     /locus_tag="VI319_13550"
     CDS             11639..12247
                     /gene="lipB"
                     /locus_tag="VI319_13550"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY6864916.1"
                     /translation="MRVQRLGLAEYVPTYEAMRRFTDARDASTPDELWVLQHPPVYTV
                     GLAGRPQHLPRGSTIPVERIDRGGQITYHGPGQAIVYTLVDLKRRALTVRGLVRLIEQ
                     AVLELLAGHGVAAERKPGAPGVYVDGAKIAALGLRVRRGCAYHGVALNVDMDLAPFRD
                     IDPCGYPGLAVTQTSALGVAESVEALGDALARRIASLLEPPR"
BASE COUNT         1982 a         4280 c         4087 g         1982 t
ORIGIN      
        1 agtcatgcgc ttcttctttt gtccgcatcg atctcgtttc atctctttcc ggatcgtaca
       61 aacgtgtctc ttgcactatc ttgccaccgt cttcaaggat ctcaatttgc cgctttgctt
      121 cgtactctat cgctcgctcc aagaatcgaa acgagtttag attctttatc tcacagcgag
      181 taccgagatt cggatcgcct ttgcgtcgca ccgatacatt ggcgtcacaa cggaaagaac
      241 cttcttgcat gttgccatca cagatgccta tccaacgcac gagcccgtgc aatgcacgcg
      301 catacgagac agcttcttct gcagatcgca tgtcgggctc ggaaacaatt tccagcagag
      361 gtgtcccggc acgattcagg tcgatacccg tcataccgtg aaaatcttcg tgcaaggact
      421 taccggcatc ttcttccaaa tgtgcgcggg ttaagcgtac gtgctttatt ccttccttca
      481 cgaggaagtt gattgttgca ttactcactg gctcgccctg tacgatcgga atttcgtact
      541 ggctgatttg atatcccttg ggaaggtctg gatagaagta gttcttccga gcaaatatcg
      601 accggcgatt tatggtgtcc cggccgccga cggcgagccc gaagcgtatc gcgcgctcca
      661 ccgcgccgcg gttcgccacg ggcagcacgc ccggcagtgc gatgtcgacc gcgctcgcct
      721 gcgtgttggg cggtgcgccg aacgcggtcg aggcgccgga gaacatcttc gagcgcgtcg
      781 agagctgcgc gtgcgtctcg aggccgacga cgatctccca ggcgctcgtc gtcatgcggc
      841 ggccttcggc gcgcgcaggt gccagtcgct cgcctgctgg tagcggtgcg cgatcccgag
      901 cagccgcgcc tccgagaaat aatcgccgac taggtgcagg ccgaccggca gcccgccctc
      961 gcccgtcccg cacgggatcg acagggccgg cagtcccgcg aggttcacgg gcacggtgta
     1021 gatgtcggag aggtacatct gcaccgggtc ggacgccttg gcgccgatcg cgaaggcgac
     1081 gccgggcgtc gtcggaccgg cgatcacgtc gcaggcgcgg aaggcctcgg cgtagtcgcg
     1141 cgcaatgagg cgccgcacct tctgcgcctt caggtagtag gcgtcgtagt agccgtgcga
     1201 gagcacgtag gtgccgacga ggatgcggcg cttgacctcc gcgccgaagc cctgcgcgcg
     1261 cgtcttgcgg tacatgtcga gcaggtccgc gtactctttc gcgcggtagc cgtagcgcac
     1321 gccgtcgtag cgcgagagat tcgacgacgc ctcggccggc gcgatcacgt agtaagcggc
     1381 gagcgacagc cggttgttcg gcaattcgat ctcgacgcgg cgcgcgccga gcttctcgag
     1441 cgccgcgatc gacgcctcga ccgccgtgcc gacctcgccc gccaggcccg cgccgaaatg
     1501 atcgcggcaa acgccgacgc gcaagccgcc gagcggcttc gttgcgcctc ccgcgagcgg
     1561 acgctcgagc tcgcgcgcga aatcctccgc gggccgctcg agcgaagtcg agtcgcgcgc
     1621 gtcgtatccc gccatgacgt tcatgagcag cgcgaggtcc tcggcggtct tcgccatcgg
     1681 cccgccctgg tcgaggctgg aggcgtaggc caccatcccg tagcgcgaga cgacgccgta
     1741 ggtgggcttg agcccgcaga tgcccgacag cgccgcgggc tggcggatcg agccgccggt
     1801 atcggtgccg gtcgcggccg gcgcgatgcg cgccgccacc gccgcggccg agccgcccga
     1861 agagccgccc ggcacgcgcg cgccgtccca gggattcttc accggcccga agtgcgagtt
     1921 ctcgttggac gagcccatcg cgaactcgtc catgttcgtc ttcccgagca cgacgcagcc
     1981 ggccgcgtcg aagcgctcga tgacgtgcgc gtcgtacggc gcgatgaagt cggcgagcat
     2041 gcgcgagccg caggtcgccg gccatccgcg agcaaggaag atgtctttgt gcgcgatcgg
     2101 tatcccggtg agcgccgtgc ccgctccggc cgcgatccgc tcgtcggccg cgcgcgcctg
     2161 cgccaggctg cgctccgggt ccaggctgat gaaggcgttg agccccgcct gcgatttctg
     2221 cgcacgcccg agaaacaatc gcgtcaaatc gacgctcgat atttcgcggc gcgcgagcgc
     2281 ggcggacagc tgcttcaggg accggtcatg catggccgga ccgcggcgcg gggcgctcta
     2341 ttcgatcacc ttcggcacga ggtacaggcc cgagtcgacc cggggggcga ccgcctggta
     2401 cggctcgcgc cggtcgagct cggtgacatt gtcctcgcgc agccgcagcg tgacgtctcg
     2461 ggcgtgcgac atcggctcga tgccgtcggt gttcaccttc tgcatctcgc cgatcatcga
     2521 caaaatgtcg ttcaattgcg actgcagcgc ctgcgtctcg gcggctccga tctcgagccg
     2581 cgcgagcatt gcgattcgtt gcacttcctc gggggacagc cgcatgaaat ttcactttag
     2641 ccgacgggta tttgcggtat tatagcgcgt ttgcgaatcc gcttcgtcta agacgctgtc
     2701 gcaaaagggg ttttgcggca ggaacagggg tggcaccgcc accggggatc aggacaaagg
     2761 cattgaggga ctcaacgaag atgttcggct ttttacgcgg gtatttctcc gacgacatgg
     2821 cgattgacct gggcacggcc aatacgctga tttacgtgcg cggcaaaggc atcgtgctcg
     2881 atgagccttc cgtcgtcgcg atccgccagg aaggcggacc gaacggcaag aagaccatcc
     2941 aggcggtcgg caaggaagcc aagcagatgc tcggcaagac gccgggcaac atcaccgcca
     3001 ttcggccgat gaaggacggc gtgatcgccg acttcaccgt caccgagcag atgctcaagc
     3061 agttcatcaa gaaggtgcac gagtcgcgcc tgttctcgcc gtccccgcgc atcatcatct
     3121 gcgtgccgtg cggctcgacg caggtcgagc gccgcgcgat ccgcgaggcc gcgaccgagg
     3181 ccggcgcgag ccaggtctac ctgatcgagg agccgatggc cgccgcgatc ggcgccgacc
     3241 tgccggtcgc cgaagcgacg ggctccatgg tcgtcgacat cggcggcggc acgaccgagg
     3301 tcggcgtgat ctcgctgggc ggcatggtgt tctccgggtc ggtgcgcgtc ggcggcgaca
     3361 agttcgacga ggcgatcatc aactacatcc gccgcaacta cggcatgctg atcggcgaga
     3421 cgaccgccga gacgatcaag aaggaaatcg gctccgcgtt tcccgggtcg gaagtgcgcg
     3481 agatggaagt gaagggccgc aacctcgccg agggcatccc gcgcagcttc accatcagct
     3541 cgaacgagat cctcgaggcg ctcaccgagc ccttgaacca gatcgtcggc gcggtgaagt
     3601 ccgcgctcga gcagacgccg cccgaactgg gtgccgatat cgccgaaaag ggcatggtgc
     3661 tgaccggcgg cggcgcgctg ctgcgcgatc tcgaccggct gctgatggaa gagacgggcc
     3721 tgcccgtcat cgtcgccgac gatccgctca cctgcgtcgt gcgcggctcg ggcaaggcgc
     3781 tcgaaaagat ggaaaagctc gggagcatct tcgccaacga atgacggaac cccgctgcat
     3841 gcatgcttga ccgcttgaag agcgccggcg cgccggcgcg cctcgcgcgt cctttccccg
     3901 ctttgcgctt tccgggcccc tgatggacta ccagccgccg ccgttcttca gccgtgggcc
     3961 cgcaccgctg gtacggctga tgttcttcat ctcgctcgcg gtgctgctca tggtgctcga
     4021 cgcgcgcttc cgctataccg agtcgctgcg ccaggtggtc gcggtcgtcg tctacccgct
     4081 gcagcgcctg gcgctgctgc ccgtcgacct cgccaacggc gcggccgact acttcacgag
     4141 cctggtcacg ctcaagcacg agaacgagca gctcaagacg cgccagctcg agtcggcgaa
     4201 cgacctgctc acgctcgagt cgctgcgcgc cgagaacgtg cagctgcggc gcatgctcga
     4261 cgcccaggag cagctgccgc gcaaggcgac tttcgccgag atcctgtaca tgagccgcga
     4321 tccgttctcg cgcaaggtcg tcatcgacaa gggcagccag cagggcctcc agaccgggca
     4381 ggccgtgatc gacgacgtcg gcgtcatcgg ccaggtgacg cgcgtctacc cgctcctctc
     4441 cgaggtgacg ctcgtcaccg accgcgagca ggcgattccc gcgcagatcg tgaggaacgg
     4501 cctgcgcgcg gtcgtctacg gcggcagcga cccggggacg ctcgagcttc gctacatgcc
     4561 caccaacgcc gacatccaga acggcgacgt gctcgtcacc tcgggcatcg acggcaccta
     4621 cccggagggg ctgccggtgg cccgggtggt gaagatcgag cgcgacgccg cgtacgcgtt
     4681 cgcgaaagta tcgtgcgtgg cgaccaccgg acccgaccgg caccggcagg tgctggtgct
     4741 cgcgtacgac tggaagcagc cgccgcctcc gccgcaggcc gcggatcagg cacccaagcc
     4801 tgccgcgaag gccaagcggc ccaagcggcc ttgagggcag cgcgcgatgg cctttgatcc
     4861 ccgcttccag ggcatcccgc ggccggtgaa cgtgggcttc atggcgctca ccgccgcggc
     4921 ggcgatgctg ttcaacatgc tgccgtggcg cgacgtgatc gggctgcccg acctgctcgc
     4981 gctggtcatc gccttctggt gcgtgcacga gccgcgcaag atgggcatcg gcttcgcctg
     5041 ggcgctcggc ctgctcatgg acgcgggcaa cggcgcgctc atgggccagc acgcgttcgc
     5101 gtacgcgatc ctcgcgttcg gcgcgatcgc gctgcaccgg cgcatcctgt ggttctcgcc
     5161 gtgggctcaa gcggcgcacg tattgatcct gcttgtcgcc agccagctgc tgatgctgct
     5221 cgtgcgcatg gcggccggcg gcgcctaccc ggggctcggc tatttcatcg gtccggtcat
     5281 cgcggcggcg ctgtggcccg tggcgaccta cgtgctgctg ctcccgcagc gccggccgcg
     5341 ccattcggac gagccgcgct cgatatgagg ttcgccgagc tcaagaacac gcagcgcgag
     5401 ctgcattatt tccagctgcg cgtgggcttc gccgggatgg cggtggtgat cgccttcggg
     5461 ctgctgttcg cgcgcttctt ctacctgcag gtcatccagc acgactacta ccacacgaag
     5521 gccgaggaca accgcatctc gatcgtgccc gtgctgccga accgcgggct gatcgtcgac
     5581 cgcaacgacg tcgtgctcgc gcgcaactac gccgcgtaca cgctcgagat cagccccagc
     5641 aaggtcgcga acctgaaggc gacgatcgac gagctcgcgg gcatcgtcga catccagccc
     5701 aaggaccgcc gccgcttcca gaagatgatg gaggagagca agacgctcga ctcgatcccg
     5761 atccgcatcc ggctcacgga cgaagaagtc gcgaagatca cggtgctcaa gtacaagctc
     5821 ccgggcgtcg acgtcaaagc gcggctgttc cgccagtacc cgctcgccga gctcgcttcg
     5881 cacgtcaccg gctacatcaa ccgcatcacc gaccgcgacc tcgagcgcat cgaggaggcg
     5941 gggctcgacg cgaactacaa gggcaccgac cacatcggga agacgggact cgagcagagc
     6001 tacgagcagg agctgcacgg caccaccggc ttcgagcagg tggagatcga cgccggcggg
     6061 cgcggcgtgc gcacgctgtc gcgcaccgcg ccgatctcgg gcaacaacct cacgctcacc
     6121 ctcgacatcc ggctgcagca ggtcgccgac gaggccttcg gcgaccggcg cggggcgctg
     6181 gtcgcgatcg agccgtcgac cggcggcatc ctcgcgttcg tgtcgaagcc cggattcgat
     6241 cccaacctgt tcgtcgacgg catcgaccct gcgaactggg acgagctgaa caactcgccc
     6301 gacctgccgc tcaacaaccg cgcgctcgcg gggctctacc cgcccgggtc gaccttcaag
     6361 ccctacatgg cgctcacggc gctcgaggtg ggcaagcgca cgccgacgca gtcgatcttc
     6421 gatcccggct acttcaactt cggcggccac cgcttccgcg acgacaaggc gggcggccac
     6481 ggctacgtcg acatgtacaa gtcgatcgtc gagtcgtgcg acacctacta ctacatcctc
     6541 ggcaacgatc tcggcatcga caccatggcg cgcttcatcg gccagttcgg cttcggccag
     6601 cgcaccggcg tcgacgtcga gggcgaggcg atcggcgtcc tgccttcgca ggagtggaag
     6661 ctcaagcgct tccgccgccc ggagcagcag aagtggtacg cgggcgagac gatcagcgtg
     6721 gcgatcggcc agggctacaa ctcgtacacg ccgctgcagc tcgcccaggc ggtggcgacg
     6781 ctcgccaaca acggcgtgat gtacaagccg cacttcgtga actacatcga gagcgcgaag
     6841 acgggcgagc gcacctacgt cgagcccgag ccggtcaaga cgatcaagct caagccccag
     6901 aacatccagt tcatcaagcg cgccatggtc ggcgtgaaca aggaaggcac cggcgccgtc
     6961 gcgttccgcg gcgcggagta cacgagcggc ggcaagaccg gcaccgcgca ggtcatcgcg
     7021 atcaagcaga ccgagaagta cgacgagaag cgcaccgccg agcgccaccg cgaccacgcg
     7081 ctgttcatcg cgttcgcgcc ggtggagaac cccaagatcg cgctcgcggt cgtggtcgag
     7141 aacgcgggct tcggcgcgcg cgcggccgcg ccgatcgcgc gccaggtgct cgattactac
     7201 ctgctcggca agcgtaaggg caagcccgcc gagaccgccg tgacgcccca gagcgacgac
     7261 gatgagagcg attgacaacc tcgtcagacg catcaccggc cgcctcgacg gcccgctgct
     7321 cggcatcgcc acggcgctcc tcgcgctcgg gctgctgacc ctctacagcg cctccaacga
     7381 gaaccccgct cgggtctccg cgcaggtcgc gaacatcctg gtcgcgttcg cgctgatgtg
     7441 ggtgatcgcg caggtgtcgc cgcagacgct gatgcgcttc gcgcttcccg tctacatgct
     7501 cgggctgctg ctcctcgtcg cggtggcgct gttcggcgac gtgcgcaacg gcgcccggcg
     7561 ctggctctcg ttcggcgtcg gcagcgtgca gccgtcggag ctgatgaaga tcgcgatgcc
     7621 gctcatgctc gcgtggtatt tccaccgcca cgagacggcg ctgcgcttcc gcgactacct
     7681 ggtcgcgatg atcctgctcg ccgtgccggt ggcgctgatc gtgcgccagc ccgatctcgg
     7741 cacggcgatg ctcgtgaccg ccgcgggctt cttcgtgatc ttcctcgccg gcctgtcgtg
     7801 gaagatcatc gtcggtgggg gggtggcgat cctcggcgcg atgccgctgc tctggggcat
     7861 gctgcacgac taccagagga agcgcgtgct gacgctgctc gatccgagcc aggacccgct
     7921 cggcgccggc taccacatca tccaggcgac gatcgccgtc ggctcgggcg gcctcgtcgg
     7981 caagggctgg ctcaacggta cgcagacgca cctcgagttc atccccgagc gctcgaccga
     8041 cttcatcttc gcggtgttct ccgaggagtt cgggctgctg gggaacggcc tgctgctcgt
     8101 cctgtacctc tcgctcgtca cgcgcggcct gatgatcgcg gcgaacgcgc cgaccttctt
     8161 cgcgcggctg ctcgccgggt cgctcaccct gacgcttttc acctacgcct tcgtgaacat
     8221 ggggatggtg agcgggctgc tgcccgtggt cggcgtgccg ctcccgttca tctcctacgg
     8281 cgggacggcg ctggtgacgc tcttcatcgc gatcgggatg ctgatgagca tccagaatca
     8341 ccgcaccctg gtgcagacgt gagcctgaac gcagatgcgg cgcgcgaccg cggagtgcgc
     8401 ggcgcgctcg tcgcgattgc cgtttccatt ctgctcgcgg cctgcggcac ctcgccgaag
     8461 cgccccggcg gctattacaa ggacgacggg cccggcgcgt cgccgccgaa gaacgtcgag
     8521 tcgacccccg acgcggcgcc gcgggtcgag ccgctgcaca agagcgcgaa caacccctac
     8581 acggtcttcg gccggcatta cgtccccgcg aagacgctgc ggccctacaa ggagcgcggc
     8641 atcgccagct ggtacggcaa gaagttccac ggccagatga catcgagcgg cgagcgctac
     8701 gacatgtaca agatgaccgc ggcgcacacg acgctgccga tcccgagcta cgcgcgcgtg
     8761 accaacctca agaacggccg cagcgtgatc gtgcgcatca acgaccgcgg cccgttccac
     8821 tcgggccgca tcatcgatct ctcgtacgcg gccgcggcca agctcggcta cgtcaatgcg
     8881 ggcagcgcgc cggtcgaggt cgaggcgctg ctgccgggcg agatgtcgcg cgccgcggaa
     8941 gagcagcccg cggcgagcgc ggcaccggtc gaggcgcccc cggccgtcgc cgccgaggcg
     9001 ctgccggccg aggccatccc cgccgcgccc gacgcgaaag gcgtctacct ccagctcggc
     9061 gcgttctccg cgcgcgacaa cgccgacgcg ctgcgcgggc gcctcgagcg cgagctgtcg
     9121 tgggtcgacc gctcgttcga ggtcctgccg gtcgacggcc tgttccgggt gcgcctcggg
     9181 ccgtatcgcg accgcggcga ggcggacggc gtcgcgctcc gtatacgcga ggccctggat
     9241 ctgaagcccg tggtcatcga gcgctagggc gccggtataa ttcttctttt ttcctccgca
     9301 gcttcgatgt cgcgcttccc gctcgctcgt tttgtcctgc ttttcgcatc cctttgcggc
     9361 agcgccgccg cccaagctcc ccagccgccc gcgatcgccg cgcgcgcctg ggtgctcatg
     9421 gacgtctcga gcggccagcc gatcacggcc cgcaacgccg acgagcgcat ggagccggcg
     9481 tcgctcacca agctgatgac cgcgtacgtc gccttcgccg cgatccggca gaagacggtc
     9541 gcgatcgacc aggcggtgcc ggtgtcggtg cgcgcgtggc gcgtgggcgg ctcgcgcatg
     9601 ttcatccagc ccaacaagaa ggtgacggtc gaggagctgc tctacggcat gatcgtcgac
     9661 tcgggcaacg acgcctgcat cgcgctcgcc gaggcgatcg cgggatccga ggaggcgttc
     9721 gtgcagatga tgaaccgcga ggcgcagcgc ctcgggatga gcaataccaa cttcaccaac
     9781 gtcagcggcc tgccggatcc gaagcactat tcgacggcga acgacctggc gctgctcacg
     9841 ggcgcgctga tccgcgactt ccccgagtac tacaagctgt actcggtgcg cgagtaccgc
     9901 tacaacaaca tcacgcagct caaccgcaac cggctgctgt ggctcgatcc caacgtcgac
     9961 ggcgtcaaga ccgggcacac cgagagcgcg ggctactgcc tggtcgcctc ggcccagcgc
    10021 gggtcgcggc gcctgatgtc ggtggtgctc ggcacgtcgt cggagacgct gcgcgcgcag
    10081 gagagccaga agctgctcaa ctacggcttc cagttcttcg acagcgtgcg gctgtaccag
    10141 aaggagcacc cgatcagcac gctcgaggtc tggaagggca gcgtctcgcg cctgcacgcg
    10201 ggcgtcctgc gcgacctctt cgtgacggtg ccgaagggcg acgcgacccg gctgaaagcg
    10261 gagctgctgt cggagcagcc gctgatcgcc ccgatcgcgc aggggcagaa ggtcggcacg
    10321 ctgcacgtca cgctggacgg caagcccctc gcggattacc cggtggtcgc gttggaggcg
    10381 gtcgggatcg cgggctggct cggtcggtcc tgggacagcc ttcgtttgat gttcaagtag
    10441 aagaggggtc gcgatgatct atctcaacgg ggacttcatg ccgctcgagc aggcgaagat
    10501 tcccgtgctc gaccgcggct tcatcttcgg cgacggcgtc tacgaggtga tcccggtcta
    10561 ttcgcggcgg ccgttccggc tgcccgggca cctggcgcgg ctgcaggcga gcctcgacgc
    10621 gatctcgatc gagaaccctc actcggggga gaagtggcgc gagctgatcg agcgcatcgt
    10681 cgccgagaac ccctgggagg accaggcggt gtacctccag gtgacgcgcg gcgtcgccag
    10741 gcgcgaccat acgttcgtgc gcggggtcgc gcccacggtc ttcatcatgt cgaacccgct
    10801 ggtgacgccg tcggccgagg tggtgaagaa cggcggcgcg gcgatcgtgc ggcccgactt
    10861 ccggtggctc cgctgcgaca tcaagaccac ctcgctgctc ggcaactgca tgctgcgcac
    10921 cgaggcggcg gacgcgggct gcaccgagtc gatcctggtg cgcgacggct tcctcaccga
    10981 ggcgtcggcg agcaacgtct tcgtggtgcg ggagggaacc gtgctcgcgc cgccgaagtc
    11041 gcacctgatg ctccccggca tcacgtacga cgtcgtgctc gagctgctcg ccgcgaacgg
    11101 cgtgccgcac gcggcgcggc cggtggcgga ggccgagttg cgcgccgccg acgagatctg
    11161 ggtcacctcg tcctcgcgcg aagtcatggc catcaccacg ctggacggca agcccgtcgg
    11221 ccgcggcgag cacgccggcc gcccgggccc ggtgttcgcg cgcgtctacg cgctctacca
    11281 gcagtacaag gcgaaggtga tgcgcgcccc ggttcatgcc tgaccccgaa gcgccgctgc
    11341 tcgccttccc ctgcgagttc ccgatcaaga tcatggggct cacgcagaaa gggttcgcgc
    11401 aggcggtgat cgaggtcgtc gcccggcacg cgcccgactt cgacccggcg acgatggaga
    11461 tgcgtccctc gcgcgagggc aagtatctct cgctcacgtg caccatccgc gccgtgtcgc
    11521 gcgagcagct cgacgcgctc taccgcgagc tgtgcgatca tcccatggtg acgatggtgc
    11581 tctgatgagc ctcgataccc tcgcgcccga agtcgcgcgt ccgcgcgccg cgccggggat
    11641 gcgcgtgcag cgcctgggcc tcgccgaata cgtccccacc tacgaggcga tgcgccgctt
    11701 caccgacgcg cgcgacgcct cgacgcccga cgagctctgg gtgctgcagc acccgcccgt
    11761 gtatacggtc ggcctcgccg ggcgcccgca gcacctgccc cgcggatcga ccatcccggt
    11821 cgagcgcatc gaccgcggcg ggcagatcac gtatcacggc cccggtcagg cgatcgtgta
    11881 cacgctcgtc gacctgaagc ggcgcgccct cacggtgcgg ggactcgtgc gcctgatcga
    11941 gcaggcggtg ctcgagctgc tcgctggcca cggcgtcgcc gccgagcgca agcccggcgc
    12001 ccccggcgtg tacgtggacg gcgcgaagat cgccgcgctc ggcctgcgcg tgcgccgcgg
    12061 ctgcgcgtac cacggcgtcg cgctcaacgt cgacatggat ctcgcgccgt tccgcgacat
    12121 cgatccctgc ggctacccgg gcctggcggt gacgcaaacc tccgcgctcg gcgtcgcgga
    12181 aagcgtcgag gcgctcggcg acgcgctcgc gcggcgcatc gcgtcgctcc tggagccgcc
    12241 tcgatgagcg cgacgaaaga aaaaggcgcc gcgaagatcc ttcgccgcac catcccgctc
    12301 gtccccgcgg cgaagctgaa gaagcccgag t
//
LOCUS       DATDMW010000324        12222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1589554, whole genome shotgun sequence.
ACCESSION   DATDMW010000324 DATDMW010000000
VERSION     DATDMW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12222
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589554"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VI319_13555"
     CDS             complement(<1..345)
                     /locus_tag="VI319_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659754.1"
                     /note="catalyzes the formation of
                     tetrahydropteroyl-L-glutamate and methionine from
                     L-homocysteine and
                     5-methyltetrahydropteroyltri-L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY6864917.1"
                     /translation="MQPVLQTTIAGSLPKPAWLAEPKKLWAPWLLEGAELERGKREAL
                     RLAVVDQESAGIDIVTDGEQTRRHFVTTFIEALDGVDFQRKRTVRIRNRYDADVPMVV
                     GPVSRRSAVFVED"
     gene            complement(349..1185)
                     /locus_tag="VI319_13560"
     CDS             complement(349..1185)
                     /locus_tag="VI319_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate 1,2-dioxygenase"
                     /protein_id="HEY6864918.1"
                     /translation="MAELAGVFASSHAPLVIRGWKDISAPNQQSLKHAYDELGRRMKA
                     ARPDVIVEISPDHWVNFFINNLPAICVGVGEEHEGPGEPWMKACPYKTIAGHPKLATH
                     IAQLAFEHDFEPAVSHHLILDHGFAIPLWKGGFDTLPPIVPIIFNDLEPPLPTVRRCL
                     AWGSMLAEAIRTYPENIRVAILATGGLSHSIGEPTMGEIDEDFDRECLRHFEAADERR
                     LVDFLNERLPTIGNGAAEVRNWVAAHGAAGKRGFELIDYCNVPEVYVGCGFASWNLGA
                     RP"
     gene            complement(1178..1459)
                     /locus_tag="VI319_13565"
     CDS             complement(1178..1459)
                     /locus_tag="VI319_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864919.1"
                     /translation="MRDYWLSKLFFDLQSTPALGVEYRADRSKVLDRYPLKPEVRAAV
                     EKDDVAYLAARVNPYLLRFYFVVCGMPEGEFLSKVRNAGADAAARSSHG"
     gene            complement(1456..2157)
                     /locus_tag="VI319_13570"
     CDS             complement(1456..2157)
                     /locus_tag="VI319_13570"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY6864920.1"
                     /translation="MTGLVSAADAYAFERERLAAEITAMARETGPETGRTRFADAVMA
                     AMHKVPRHRFVSPGDEPFAYENRPLPIGEGQTISQPYIVALMTELLDPRPEHDVLEVG
                     TGSGYQTAVLAETGARVYTLEVVDALARRASELLVELGYANVTARTGDGYEGWPEHAP
                     FDSIIVTAAASDVPQPLVNQLKAGGRLVIPVGGAGDVQHLLLVDKRADGTTARRRTLP
                     VRFVPLVRARGKIAP"
     gene            complement(2200..2901)
                     /locus_tag="VI319_13575"
     CDS             complement(2200..2901)
                     /locus_tag="VI319_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864921.1"
                     /translation="MSDALKLSGVRAGYGETVVLEDVGFTLAERGTLAVLGRNGVGKT
                     TLLSTIMGHTTFHSGSVEFRGRPVAALPVYSRCRLGMGYVPQAREIFPSLTVEENLAI
                     ADRRGRWTLERVYELFPRLAERRAHMGNQISGGEQQMLAIGRALMGNPSLLLMDEPFE
                     GLAPIIVEALLAALQLLIREDALTVVLVEQHAKLALQVTQEALVLNRGRIAYHGSSAA
                     LLSDPHRLTSLIVAQ"
     gene            complement(2894..3649)
                     /locus_tag="VI319_13580"
     CDS             complement(2894..3649)
                     /locus_tag="VI319_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864922.1"
                     /translation="MSAAALETRGLCKSFGALTVADDIDFRLEPGARHALIGPNGAGK
                     TTFVNMLTGALRPSSGRILLQGEDVTAVRQAARVKRGLGRTFQINQLFRRLSVLDNVA
                     LGVAERRGVARRMWKPASRHAEVRDESLALLASLGLADDADQRVLDLPYGKQRLVEIA
                     IALGLKPKVLLLDEPAAGVPSLESHRILEVLDRLPADISILIIEHDMDLVFRFARRIT
                     VLVQGEVLLEGAPEEIAKDRRVHQVYLGEQRHV"
     gene            complement(3646..4623)
                     /locus_tag="VI319_13585"
     CDS             complement(3646..4623)
                     /locus_tag="VI319_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6864923.1"
                     /translation="MSAHAGGYSANPRIRWIEWLPFVLAIAAFFALPEYLSLGARILI
                     YILYALSLDLILGYAGIITLGHSAYFGLGAYVAGILGARLGVSDPLVQLVAAAAAGGL
                     LGIATGAIILRTRALTLLMLTLAITSVCLEIANKATPVTGGADGLSGIVVAPILGLFR
                     FDLFGKTAFLYCLVALLIGWLAVRRLIYSPFGASLTGLRENPLRMHAIGAPVYWRLVL
                     VYTISTTLAGVAGALITQTNQFVGLNVLGFESGGELLVMLVLGGVGRLYGAFVGPLVY
                     LVAQDFLAKQFPEYWYLGIGAMLIVVVMFARGGILGIVDSIVRRLWRRA"
     gene            complement(4620..5498)
                     /locus_tag="VI319_13590"
     CDS             complement(4620..5498)
                     /locus_tag="VI319_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6864924.1"
                     /translation="MEGFLSSLVSVAFHGTAFGMILYVISVGLSVTMGLMGFANLAHG
                     VFAMAGGYVLTSMISRYGVPFPLALAIAFAVVGAASVVLERLLYSRLYAASDLEQVMF
                     TIGLIFVAVAVARMIFGTLQQPVILPDYLKGQFPLLGRDFPTYRVFIIAFSAVMVGVL
                     WFGVERTRWGAMVRAAVDNRAMAQSIGIDTGRLFTLTFALGSGLAGLGGALGAEIIAI
                     QPTYPFENLVYFLIVVAVGGLGTLRGPFVAALLIGIADTAIKYWYPQYGAFLIYVATI
                     AALLWRPAGLFGRQHT"
     gene            complement(5528..6721)
                     /locus_tag="VI319_13595"
     CDS             complement(5528..6721)
                     /locus_tag="VI319_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864925.1"
                     /translation="MNLRTTWKIVAALALALGAAPVSAQTVKIGVVNTYSGPNATLGE
                     YIDKGIKLYTKLHQKDLPPGVKVELVVRDDGGPNPDKAKQLAQELIVRDRVQILAGTV
                     FTPNAAAIAPLTSEAKVPYVLMNAGGSVLTTLSPYIARTSFTLWQSSYPLGQWAAKKY
                     KRAYIAVSDFAAGHDCEAAFEKAFTDGGGQIVGKVRMPLANPDFVPFMQRVKDAKPEV
                     LFAFIPAGKQATAIMKAYGELELDKAGIKFIGPGDITTDEELPNMGDVPLGVITAHHY
                     SAAAGRPANKAFVAAWKKEYGQDSNPNFLAVGGWDGMDAIYTAIREQKGRIDPVRTME
                     ILKKYKNPNSPRGPIAIDPDTRDIVQNEYLREVRKVGGKLANVELETIGTAVKDPWKE
                     INHKK"
     gene            complement(6748..7197)
                     /gene="paaI"
                     /locus_tag="VI319_13600"
     CDS             complement(6748..7197)
                     /gene="paaI"
                     /locus_tag="VI319_13600"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEY6864926.1"
                     /translation="MEDPHPSQDAAEAAVKAMWRDDRCAAALGMRVLEVSPGAGRVEM
                     RVREDMLNGHGTTHGGMIFTLADAAFAIASNSRGRRALAAGCSIEFLRPAVAGDLLVA
                     SASEQSLGARSGIYDVRVENQHGETVALFRGKAALLRGSWTDDTPPV"
     gene            7311..8519
                     /locus_tag="VI319_13605"
     CDS             7311..8519
                     /locus_tag="VI319_13605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY6864927.1"
                     /translation="MFLENRFERLVDRIRRSASIPLRFELWNGRAFDLASDPTVRVKL
                     PRPSALRFLVSPDLLKLGEAYVEGHIGVDGPIMEVFRVAESFAKSATAKGRSGLRRFA
                     RHSKQRDRKAIEYHYDVSNAFYSLFLDRNMVYSCAYFRDEADTLDQAQEQKLDHILNK
                     LMLQPGERFLDIGCGWGALVIRAAKKYGARAVGVTLSRNQHEHAMRRIREEGLEGRCE
                     VRLQDYRDIPYEGGYDKIASVGMFEHVGLRNLQAYFGTIRALLKDGGLAMNHGITSAD
                     PESRAVGLGAGEFIHRYVFPHGELPHISIVLREMTRAGLEAADVESLRRHYARTCLEW
                     ARRLEANRDLAIASAGDRRYRIWQVYLAGCAHGFARGWMNIYQVLVRKDRDALNPLPM
                     TRDYMYGTKG"
     gene            complement(8532..9074)
                     /locus_tag="VI319_13610"
     CDS             complement(8532..9074)
                     /locus_tag="VI319_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864928.1"
                     /translation="MYADTLKLIASLPGRRPSPHLPEQLHVLFRRLHESTDPDAREAI
                     EDEIWRLWMSHPEDAVATDLERATRAMTAQDFGVADQILRGVVGSHPGYAEAWNKRAT
                     LYYIAGKDAECVPYIHRTLELEPRHYGALCGFGEILLARGETDAALFVFERALRIHPH
                     LDTARAAIARLLTSRTSTLQ"
     gene            complement(9081..12158)
                     /locus_tag="VI319_13615"
     CDS             complement(9081..12158)
                     /locus_tag="VI319_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY6864929.1"
                     /translation="MRALPGYAELHCLSSFSFLRGASRPEELIERAAVLGYRALAVTD
                     ECSVAGVVRAHEATKEAAAKELGLRLIVGSEIRLDGGAKLVILATDREGYGELCQLVT
                     LGRRRKEKGSYSLAWSDLDGGMPGCLALLVSENEEHARALAERLRDRAWIAAELLCGP
                     DDRAQLARLRALARASGLPLVAAGDVHMHVRSRRPLQDTLTAIRLGTPVHACGHALHP
                     NAERHLRARVRLAQLYPPELLAETVAIAERCAFSLDTLRYEYPEELVPEGETPTSHLR
                     ALALDGMRARYPGGVPDAVAGLVEKELALIAELKYEPFFLTVHDVVRFARSRKILCQG
                     RGSAANSAVCYALGITEVDPTRQRLLFERFVSKERNEPPDIDVDFEHQRREEVIQYLY
                     EKYGRARAALAATVICYRPRSALRDVGKALGLDPPQVDRIARSVSWWDGRADLEQRLR
                     DAGFDPGLPTIARLVALAILLLGFPRHLSQHVGGFVISRGPLGRLVPIENAAMPERSV
                     IQWDKDDLESLGLLKVDVLGLGMLSAVRRALDLVNGLRGTAHTMASLLSAGEDPAVYE
                     MIRRADTIGVFQIESRAQMSMLPRLAPRDFYDLVIEIAIVRPGPIQGDMVHPYLRRRE
                     GKEPIEYPNDAVKAVLARTLGVPIFQEQVMELAVVAAGFTPGEADQLRRAMAAWKRRG
                     GLEPFQQKLKDGMRARGIPEAFADRIYGQILGFGEYGFPESHSVSFALIAYVSAWIKR
                     HEPAAFLAALLNSQPMGFYSPSQLVQDARRHGVEVLPVDATVSGWECALEGETVRLGL
                     LMVQGLSEAAAKRLVEARGGRAFESVDDLAHRALLDRRDLKCLAAAGALAPLAGHRRH
                     AHWAVAGVQTAGHILREAPVAETGPALAPPTEGEDLVADYASLGLTLGRHPLALLRRR
                     LARMRFSTAADLRALPSGRPARAAGIVTGRQRPDTASGAVFVTLEDETGYVNVIVWRD
                     LTERQRRELLGARLMGVEGVLEREGEVVHLIARRLLDHSALLGRLATGSRDFH"
BASE COUNT         1871 a         4222 c         4343 g         1786 t
ORIGIN      
        1 gtcctcgacg aacacggccg agcgccgcga cacgggaccc acgaccatgg ggacgtcggc
       61 atcgtagcgg ttgcggatgc gcaccgtgcg cttgcgctgg aaatcgacgc cgtcgagcgc
      121 ctcgatgaag gtcgtgacga agtggcggcg cgtctgctcg ccgtcggtga cgatgtcgat
      181 gcccgcgctc tcctggtcga ccaccgcgag ccgcagcgcc tcgcgcttgc cgcgctcgag
      241 ctccgcgcct tcgagcagcc acggcgccca cagcttcttc ggctcggcga gccaggccgg
      301 cttcggcagg ctgccggcga tggtcgtctg cagcaccggc tgcatggcct agggacgcgc
      361 cccgagattc caggacgcga agccgcaccc gacatacacc tcgggcacgt tgcagtagtc
      421 gatcagctcg aagccgcgct tgcccgcggc gccgtgcgcc gcgacccagt tgcgcacctc
      481 ggccgcgccg ttgccgatgg tcggcaggcg ctcgttcagg aaatcgacga ggcggcgctc
      541 gtccgccgcc tcgaaatgac gcaggcactc gcggtcgaag tcctcgtcga tctcgcccat
      601 cgtcggctcg ccgatcgagt gcgaaaggcc gccggtggcg aggatggcga ctcggatgtt
      661 ctcgggatag gtgcggatcg cctcggccag catcgagccc cacgcgaggc agcggcgaac
      721 cgtggggagg ggcggctcca ggtcgttgaa gatgatcggc acgatcggtg gcagcgtatc
      781 gaagccgccc ttccacagcg ggatggcgaa gccgtgatcg aggatgaggt gatgcgacac
      841 cgccggctcg aaatcgtgct cgaacgcgag ctgcgcgatg tgggtcgcga gcttggggtg
      901 cccggcgatg gtcttgtacg ggcaagcctt catccacggc tcgcccgggc cttcgtgctc
      961 ttcgcccacg cccacgcaga tcgcgggaag attgttgatg aagaaattga cccaatgatc
     1021 gggggagatc tcgacgatca cgtcgggacg cgccgccttc atgcggcgtc cgagctcgtc
     1081 gtacgcgtgc ttcaggctct gctggttcgg cgcgctgatg tccttccagc cgcggatcac
     1141 gagcggcgcg tggctggagg cgaagacccc ggcgagctca gccatggctg gacctcgcgg
     1201 cggcgtcggc gcccgcgttg cggaccttcg acaggaactc gccctcgggc atgccgcaga
     1261 cgacgaaata gaagcgcagc aggtacgggt tcacgcgcgc cgcgaggtac gcgacgtcgt
     1321 ccttctcgac cgccgcgcgc acctcgggct tcagcgggta gcgatccagg accttgctgc
     1381 ggtcggcccg gtactcgacg cccagcgcgg gcgtgctctg caggtcgaaa aagagcttgc
     1441 tcagccagta gtctctcatg gcgctatttt gccacgggcg cgaacgagcg gcacgaagcg
     1501 caccggcagc gtgcgccggc gcgcggtggt gccgtcggcc cgcttgtcga cgagcagcag
     1561 gtgctgcacg tcgcccgcgc cgccgaccgg gatcaccagc cgtccgccgg ccttcagttg
     1621 gttgacgagc ggctgcggca cgtcggacgc ggccgccgtg acgatgatgg aatcgaaggg
     1681 cgcgtgctcc ggccagcctt cgtagccgtc gccggtgcgg gcggtgacgt tggcgtagcc
     1741 gagctccacg agcagctccg acgcgcggcg cgccagcgcg tcgaccacct cgagcgtgta
     1801 cacccgggcg ccggtctccg cgagcaccgc ggtctggtag cccgagccgg tgccgacctc
     1861 caggacgtcg tgttctgggc gcgggtcgag cagctcggtc atcagtgcga cgatgtacgg
     1921 ctgcgagatc gtctggcctt cgccgatggg cagcggccgg ttctcgtacg cgaagggctc
     1981 gtcgcccggc gacacgaagc ggtgcctcgg caccttgtgc atcgccgcca tcaccgcgtc
     2041 ggcgaagcgc gtgcggcccg tctcggggcc ggtctcgcgc gccatcgccg tgatttcggc
     2101 ggcgaggcgt tcgcgctcga aggcgtaggc gtcggcggcg gagacgagcc cggtcatggc
     2161 gatgggaaca gggttgcgct gcggccgcgc cggtaccggc tactgcgcga cgatcaggct
     2221 cgtcagccgg tgcggatccg aaagcagcgc ggcgctcgaa ccatgatacg cgatgcggcc
     2281 gcggttgagg acgagcgcct cctgcgtcac ttgcagggcg agcttggcgt gctgctcgac
     2341 cagcaccacg gtgagcgcgt cctcgcggat cagcagctgc aacgccgcga gcagcgcctc
     2401 gacgatgatc ggcgccaggc cctcgaaggg ctcgtccatc agcagcagcg acgggttgcc
     2461 catgagcgcg cgtccgatcg cgagcatctg ctgctcgccg ccggagatct ggttgcccat
     2521 gtgcgcgcgt cgctcggcga gccgcgggaa cagctcgtac acgcgctcga gcgtccagcg
     2581 gccgcggcgg tcggcgatgg cgaggttctc ctcgaccgtg agcgacggga agatctcgcg
     2641 cgcctgcggc acgtagccca tgccgaggcg gcagcgcgaa tacacgggaa gggccgcgac
     2701 gggacgcccc cggaactcga ccgacccgga atggaacgtc gtgtgtccca tgatcgtgga
     2761 cagcagcgtc gtcttgccca ccccgttgcg gccgagcacg gcgagcgttc cgcgctcggc
     2821 gagcgtgaag ccgacgtcct cgagcacgac ggtctcgccg tagcccgcac gcaccccgga
     2881 gagcttcagc gcgtcagaca tggcgctgct cgccgagata gacctggtgc acgcggcggt
     2941 ccttcgcgat ctcctcgggc gcgccttcga gcaggacctc gccctgcacc agcacggtga
     3001 tgcggcgtgc gaagcggaac acgaggtcca tgtcgtgctc gatgatcagg atcgagatgt
     3061 cggccggcag ccggtcgagc acctcgagga tccggtgcga ctcgagcgag ggcacgccgg
     3121 ccgcgggctc gtcgagaagc agaaccttcg gcttgaggcc gagcgcgatc gcgatctcga
     3181 ccaggcgctg cttgccgtag ggcaggtcga ggacgcgctg atcggcgtcg tcggcgagcc
     3241 ccaggctcgc gagcagcgcg aggctctcgt cgcgcacctc cgcgtgccgg ctcgcgggct
     3301 tccacatccg acgcgcgacg ccgcgccgct ccgcgacgcc gagcgcgacg ttgtcgagca
     3361 ccgagagccg ccggaagagc tggttgatct ggaaggtgcg cccgaggccg cgcttcacgc
     3421 gcgcggcctg gcgcacggcg gtgacgtcct cgccctgcag caggatgcgg cccgacgacg
     3481 gacgcagcgc cccggtcagc atgttcacga acgtggtctt tcccgccccg ttcggcccga
     3541 tgagcgcgtg ccgcgcgccg ggctcgagcc ggaagtcgat gtcgtcggcg accgtgagcg
     3601 cgccgaagct cttgcacagg ccgcgcgtct cgagcgccgc ggcgctcatg cgcgcctcca
     3661 cagccgccgc acgatcgaat cgacgatccc gaggatgccg ccgcgcgcga acatcaccac
     3721 gacgatcagc atggcaccga tgccgaggta ccagtactcg gggaactgct tcgcgaggaa
     3781 gtcctgcgcg accaggtaga cgagcggccc gacgaaggcg ccgtagaggc ggccgacgcc
     3841 cccgagcacg agcatgacca gcagctcgcc gcccgactcg aagcccagga cgttcaggcc
     3901 gacgaactgg ttggtctgcg tgatcagcgc ccccgcgacg cccgcgagcg tggtcgagat
     3961 cgtgtacacg agcaccaggc gccagtacac gggggcgccg atcgcgtgca tgcgcaaggg
     4021 gttctcgcgc aggccggtca gagaggcgcc gaacggcgag tagatcagcc gccgcacggc
     4081 gagccagccg atcagcagcg cgaccaggca gtagaggaag gcggtcttgc cgaacaggtc
     4141 gaagcggaac agcccgagga tgggcgcgac gacgatgccg gagaggccgt cggcgccccc
     4201 ggtgaccggc gtcgccttgt tcgcgatctc gaggcacacc gacgtgatcg cgagcgtcag
     4261 catgagcagc gtcagcgcgc gcgtgcgcag gatgatggcg ccggtcgcga tcccgagcag
     4321 cccgcccgcg gcggcggcgg cgaccagctg caccagcggg tcgctcacgc cgagccgcgc
     4381 gccgaggatg ccggcgacgt aagcgcccag cccgaagtac gcgctgtggc cgagcgtgat
     4441 gatcccggcg tagccgagga tcagatcgag cgagagcgcg tacaggatgt agatcaggat
     4501 gcgcgcgccg agcgagagat actcgggcag cgcgaagaag gccgcgatcg cgagcacgaa
     4561 cggcagccat tcgatccagc ggatgcgcgg gttcgcgctg tagccgcccg cgtgcgcgct
     4621 catgtgtgct gccgcccgaa caggccggcc ggccgccaca gcagggccgc gatcgtcgcg
     4681 acgtagatca ggaacgcgcc gtactgcggg taccagtact tgatcgccgt gtcggcgatg
     4741 ccgatcagca gcgccgcgac gaacggcccg cgcagcgtcc cgagcccgcc gacggccacg
     4801 acgatgagga agtacacgag gttctcgaac gggtaggtcg gctggatggc gatgatctcg
     4861 gcgccgaggg cgccgcccag gcccgcgagc ccgctgccga gcgcgaaggt cagcgtgaag
     4921 agcctgccgg tgtcgatccc gatcgactgc gccatggcgc ggttgtcgac ggccgcgcgc
     4981 accatcgcgc cccagcgcgt gcgctcgacc ccgaaccaga gcacgccgac catcaccgcg
     5041 ctgaacgcga tgatgaacac gcggtaggtg gggaaatcgc gtccgaggag cgggaactgc
     5101 cccttgagat agtcggggag gatcaccggc tgctgcagcg tgccgaagat catccgcgcg
     5161 accgcgaccg cgacgaagat caggccgatg gtgaacatga cctgctccag gtcgctcgct
     5221 gcgtagagcc gcgagtagag caggcgctcg agcaccacgc tcgccgcgcc gacgacggcg
     5281 aaggcgatcg cgagcgcgag cgggaagggc acgccgtagc ggctgatcat cgacgtgagc
     5341 acgtagccgc ccgccatcgc gaacaccccg tgcgcgaggt tggcgaagcc catcagcccc
     5401 atcgtgaccg acaggcccac ggagatcacg tacaggatca tgccgaaggc cgtgccgtgg
     5461 aacgccacgc tgaccagcga cgagaggaaa ccctccactc agtctcccgg gcggagccgg
     5521 cgccgtccta cttcttgtgg ttgatctctt tccaagggtc cttcaccgcc gtgccgatgg
     5581 tctccagctc gacgttggcg agcttgccgc cgaccttccg cacctcgcgc aggtactcgt
     5641 tctgcacgat gtcgcgcgtg tcggggtcga tcgcgatcgg gccgcgcgga ctgttcgggt
     5701 tcttgtattt tttcaggatc tccatcgtgc gcaccggatc gatcctgccc ttctgctcgc
     5761 ggatcgccgt gtagatcgcg tccatgccgt cccagccgcc gaccgcgagg aagttcgggt
     5821 tcgagtcctg gccgtactcc ttcttccagg cggcgacgaa cgccttgttg gccgggcgcc
     5881 cggccgcggc cgagtagtgg tgggcggtga tcacgccgag cggaacgtcg cccatgttgg
     5941 gcagctcctc gtcggtcgtg atgtcgccgg gcccgatgaa cttgatgccc gccttgtcga
     6001 gctcgagctc gccgtaggcc ttcatgatcg cggtcgcctg cttgcccgcg ggaatgaacg
     6061 cgaacagcac ctcgggcttc gcatccttca cgcgctgcat gaaggggacg aagtccgggt
     6121 tggcgagcgg catgcgcacc ttgcccacga tctggccgcc gccgtcggtg aacgccttct
     6181 cgaacgcggc ctcgcagtcg tggccggcgg cgaagtcgct gaccgcgatg taggcgcgct
     6241 tgtacttctt agccgcccat tggccgagcg ggtacgagga ctgccacagc gtgaacgacg
     6301 tgcgcgcgat gtacggcgac agcgtggtaa ggaccgagcc gccggcgttc atcagaacgt
     6361 acgggacctt ggcctcgctg gtgagcggcg cgatcgcggc ggcattcggg gtgaagacag
     6421 tgcccgccag gatctgcacc ctgtcgcgca cgatcagctc ctgggcgagc tgcttcgcct
     6481 tgtcggggtt cgggccgccg tcgtcgcgca ccaccagctc gaccttcacg ccggggggca
     6541 ggtccttctg gtgcagcttc gtgtagagct tgatgccctt gtcgatgtat tcgcccagcg
     6601 tcgcgttcgg gcccgagtag gtgttgacca cgccgatctt caccgtctgc gcggagacgg
     6661 gcgcggcgcc cagcgcgagc gccagtgcgg cgacgatctt ccatgtcgta cgaagattca
     6721 tgactcctcc gggaaagtcg gcagattcta tacgggcggc gtgtcgtcgg tccacgagcc
     6781 gcgcaggagg gcggccttgc cgcggaagag ggcgacggtc tcgccgtgct ggttctcgac
     6841 gcgaacatcg tagataccgg agcgcgcgcc gagggactgc tcgctcgcgc tcgcgaccag
     6901 caggtcgccg gcgaccgcgg ggcgcaggaa ctcgatcgag cagccggcgg ccagcgcgcg
     6961 ccggccgcgg ctgttcgagg cgatcgcgaa ggcggcgtcg gcgagcgtga agatcatgcc
     7021 gccgtgggtg gtgccgtgcc cgttcagcat gtcctcgcgc acgcgcatct cgacccggcc
     7081 ggcgccgggg ctgacctcga gcacgcgcat gccgagcgcg gcggcgcagc ggtcgtcgcg
     7141 ccacatggcc tttaccgccg cttcggccgc atcctggctc gggtgtggat cttccatgcc
     7201 gtgttcctgg aacgtgggcg ggcgcccgcg gtcgaactcg cggacggagc ttccggcgcg
     7261 cgaggcgcgc ccgtgtagcc cgatgctatg atatccgccg ggagccgcgc atgttcctcg
     7321 agaaccgatt cgaacgcctg gtcgatcgca ttcgccggtc cgcctcgatc ccgctcaggt
     7381 tcgagctctg gaacggtcgc gccttcgatc tcgcctccga cccgaccgtc agggtgaagc
     7441 ttccgaggcc ctccgcgctg cgcttcctcg tgtcgcccga cctgctcaag ctcggcgagg
     7501 cgtacgtcga gggccacatc ggcgtcgacg gtcccatcat ggaggtgttc cgggtcgccg
     7561 agagcttcgc caagagcgcc acggcgaagg ggcgcagcgg gctgcgccgg ttcgcccgcc
     7621 atagcaagca gcgcgaccgc aaggcgatcg agtaccacta cgacgtctcg aacgcgttct
     7681 actcgctgtt cctcgaccgc aacatggtgt actcctgcgc ctacttccgc gatgaggccg
     7741 acacgctcga ccaggcgcag gagcagaagc tcgaccacat cctcaacaag ctgatgctgc
     7801 agcccggcga gcgcttcctc gacatcggct gcgggtgggg cgcgctcgtc atccgcgcgg
     7861 cgaagaagta cggcgcgcgc gcggtcggcg tgacgctgtc gcgcaaccag cacgagcacg
     7921 ccatgcgccg catccgcgag gagggcctgg agggccgctg cgaggtcagg ctgcaggact
     7981 accgcgacat cccgtacgag ggcggctacg acaagatcgc gagcgtcggc atgttcgagc
     8041 atgtcgggct gcgcaacctg caggcctact tcggcacgat ccgcgcgctg ctcaaggacg
     8101 gcggcctggc gatgaatcac ggcatcacct cggccgaccc ggagagccgc gcggtcggcc
     8161 tcggggcggg cgagttcatc catcgatacg tcttcccgca cggcgagctg cctcatatct
     8221 cgatcgtgct gcgcgagatg acgcgcgcgg gcctggaggc ggccgacgtc gagtcgctgc
     8281 gccggcatta cgcgcgcacc tgcctcgaat gggcgcggcg cctcgaggcg aaccgcgacc
     8341 tggcgatcgc ctcggccggc gaccggcgct atcgcatctg gcaggtctat ctcgcgggct
     8401 gcgcgcacgg cttcgcgcgc ggctggatga acatctacca ggtgctcgtg cgcaaggacc
     8461 gcgacgcgct caacccgctg ccgatgacgc gcgactacat gtacggcacg aagggctgac
     8521 gacgcacgcc gctactgcag cgtcgaggtt ctcgaggtga ggagccgagc gatcgcggcg
     8581 cgcgccgtgt cgaggtgcgg atggatgcgc agcgcgcgct cgaagacgaa gagcgccgca
     8641 tcggtctcgc cgcgcgccag caggatctcg ccgaagccgc acagcgcccc gtagtggcgc
     8701 ggctcgagct cgagggtgcg gtggatgtag gggacgcact ctgcgtcctt ccccgcgatg
     8761 tagtacagcg tcgcgcgctt attccaggcc tcggcgtagc cggggtggct gccgacgacg
     8821 ccgcgcagga tctggtcggc gacgccgaag tcctgcgccg tcatcgcgcg cgtcgcgcgc
     8881 tccaggtcgg tcgcgacggc gtcctcggga tggctcatcc atagccgcca gatctcgtcc
     8941 tcgatcgctt cgcgcgcgtc ggggtccgtg ctttcgtgga ggcggcggaa gagaacgtgc
     9001 aattgctccg gcagatgcgg ggagggccgc cgtccgggca gcgaggcgat gagcttcagc
     9061 gtgtcggcgt acatgtcgtc ctagtgaaaa tcgcgcgatc ccgtcgcgag gcgtcccagg
     9121 agcgcgctgt ggtccagcag ccgcctggcg ataagatgca cgacttcgcc ctcgcgttca
     9181 agcacgcctt ccacccccat caggcgtgcg cccaatagtt cacgccgctg gcgctcggtg
     9241 aggtcgcgcc acacgatcac gttgacatag ccggtctcgt cctcgagcgt gacgaacacc
     9301 gcgcccgacg cggtgtcggg gcgctggcgg ccggtgacga tgccggcggc gcgcgccggg
     9361 cgcccgctcg gcagcgcgcg caggtcggcg gcggtcgaaa aacgcattcg cgcgaggcgc
     9421 cgccgcagca gcgcgagcgg atggcggccg agcgtcagcc cgaggctcgc gtagtcggcg
     9481 acgaggtcct cgccctcggt cggcggcgcg agcgcggggc cggtttccgc gaccggcgcc
     9541 tcccggagga tatggcccgc agtctggaca ccggcgactg cccaatgcgc gtggcgccgg
     9601 tgcccggcga gcggtgcgag cgctccggcc gccgcgaggc acttcaggtc gcgccgatcg
     9661 agaagggcgc ggtgggcgag atcgtctacc gattcgaagg cacgtcctcc gcgcgcttcc
     9721 accaggcgct tcgccgccgc ctcggagagg ccctggacca tgagcagccc gaggcgcacc
     9781 gtctcgccct cgagcgcgca ttcccagccg ctgacggtcg cgtcgacggg cagcacctcg
     9841 acgccgtgcc ggcgcgcgtc ctgcaccagc tgcgacggcg agtagaagcc catcggctgg
     9901 ctgttcagga gtgcggcgag gaaggccgcg ggctcgtgcc gcttgatcca cgccgagacg
     9961 tacgcgatca gcgcgaagct caccgagtgc gactcgggga agccgtactc gccgaagccg
    10021 aggatctggc cgtagatgcg gtcggcgaag gcctccggga tgccgcgcgc gcgcatgccg
    10081 tccttgagct tctgctggaa gggctcgagg ccgccgcggc gcttccacgc cgccatcgcg
    10141 cgccgcagct gatcggcctc gcccggcgtg aagccggcgg cgacgaccgc gagctccatc
    10201 acctgctcct ggaagatcgg cacgccgagc gtgcgcgcga gaaccgcctt caccgcgtcg
    10261 ttcgggtact cgatcggctc cttgccctcg cgccggcgca gataggggtg caccatgtcg
    10321 ccttggatgg gacccgggcg cacgatcgcg atctcgatca cgaggtcgta gaagtcgcgc
    10381 ggcgcgaggc gcggcagcat cgacatctgc gcgcgcgact cgatctggaa cacgccgatc
    10441 gtgtcggcgc gccggatcat ctcgtagacc gcgggatcct cgccggccga gagcagggac
    10501 gccatggtgt gcgccgtgcc gcgcaggccg ttgacgagat cgagcgcgcg gcgcacggcc
    10561 gacagcatcc cgaggccgag cacgtcgact ttcagcaatc ccaggctctc gagatcgtcc
    10621 ttgtcccact ggatgacgct gcgctcgggc atcgccgcgt tctcgatcgg cacgaggcgg
    10681 ccgagcggcc cgcgcgagat gacgaagccg ccgacgtgct gcgagagatg tcgcggaaag
    10741 ccgagcaaca ggatcgccaa agcgacgagc cgcgcgatcg tcggcaaccc ggggtcgaag
    10801 cccgcgtcgc gcaggcgctg ctcgaggtcg gcgcgcccgt cccaccacga caccgacctg
    10861 gcgatgcgat cgacctgcgg gggatcgagc ccgagcgcct tgccgacgtc gcgcagcgcg
    10921 ctcctcggcc ggtagcagat caccgtcgcg gcgagcgcgg cgcgcgcgcg cccgtatttt
    10981 tcgtagagat actggatgac ctcctcgcgg cgctggtgct cgaagtcgac gtcgatgtca
    11041 gggggctcgt tgcgctcctt cgacacgaag cgctcgaaga gaagccgctg gcgcgtcggg
    11101 tcgacctcgg tgatgccgag cgcgtagcag acggccgagt tcgccgccga gccgcgtccc
    11161 tggcacagga tcttcctgct cctggcgaag cgcacgacgt cgtgcacagt caggaagaac
    11221 ggctcgtact tcagctccgc gatcagcgcc agctccttct cgaccagccc ggcgaccgcg
    11281 tccgggacgc ctccgggata gcgcgcccgc atgccgtcga gcgcgagcgc gcgcaaatgg
    11341 ctcgtcggcg tctcgccctc gggcaccagc tcctcggggt actcgtagcg cagcgtgtcg
    11401 agcgagaacg cgcagcgctc ggcgatcgcc acggtctcgg cgagcaactc gggcgggtag
    11461 agctgcgcca ggcgcacgcg cgcgcgcaga tgccgctcgg cgttgggatg cagtgcgtgg
    11521 ccgcaggcgt gcaccggcgt gccgagccgg atcgcggtca gcgtgtcctg cagcggccgg
    11581 cgcgagcgca cgtgcatgtg cacatcgccc gcggcgacca gcggcaggcc cgaagcgcgc
    11641 gcgagggcgc gcagtcgggc gagctgcgcg cggtcgtcgg gcccgcacag gagctcggcg
    11701 gcgatccagg cgcggtcccg caggcgctcc gccagcgccc gcgcatgctc ctcgttctcc
    11761 gagaccagca gcgccaggca ccccggcatc ccgccgtcca gatcgctcca ggcgagcgag
    11821 tagctccctt tctccttgcg ccgccgtccg agcgtgacga gctggcacag ctcgccgtag
    11881 ccttcgcggt cggtcgcgag gatcacgagc ttcgctccgc cgtcgagtcg gatctcggag
    11941 ccgacgatga gccgaaggcc gagctccttc gccgccgctt ctttcgttgc ctcgtgcgcg
    12001 cgcacgacgc cggcgaccga gcattcgtcg gtgacggcga gcgcgcggta gccgagcacc
    12061 gccgcgcgct cgatcagctc ctcgggccgg gatgcgccgc gcaggaagct gaagctcgac
    12121 aggcagtgca gctcggcgta gccggggagg gcgcgcattt gtcgatcaga catcaggcga
    12181 acaccccgtg caggtaccag ccgccgtcgc cgcggcggtc gc
//
LOCUS       DATDMW010000325         6289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_96820, whole genome shotgun sequence.
ACCESSION   DATDMW010000325 DATDMW010000000
VERSION     DATDMW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96820"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VI319_13620"
     CDS             <1..507
                     /locus_tag="VI319_13620"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY6864930.1"
                     /translation="DTKDEPVLHAQGVPPFGANGVGFNKAFDTLFVANTGNDTVVTVP
                     ASGGVVGNANVLTNSINGADGLILDEQDNIWVCANQGDEIVVIDKTGKVISKLGDFNG
                     IDRDGAPIGFLFPASLVRHGGFIYVTNLSLDLRPVVGAQAVDSQWAARVTTNTISRIR
                     AKILRNPD"
     gene            complement(577..1119)
                     /locus_tag="VI319_13625"
     CDS             complement(577..1119)
                     /locus_tag="VI319_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY6864931.1"
                     /translation="MPADQRGFTYLTILFAVAFIGIGLAITGEVWHTAALRDKESQLL
                     YAGNQYRRAIERYYLSGPRQYPRTLEDLLKDPRKPNVQRYLRRLYFDPVTGSSEWGIV
                     KAPDGGVMGVYSLSDVTPMKTAGFDLRNSAFEGAGKYSDWKFVYDPSKQQAIVPGAPA
                     PGAANPQQPIAPMPAPPPAR"
     gene            complement(1122..1496)
                     /locus_tag="VI319_13630"
     CDS             complement(1122..1496)
                     /locus_tag="VI319_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6864932.1"
                     /translation="MRRTGGFTLIELLVVLAIIALLLTIALPRYFGSLDRSKETVLRE
                     DLHQMRDAIDKYYGDRGKYPENLDALVSEKYLRSVPVDPITEKKDTWVVVPPQDPQKS
                     GMYDVKSGAPGQASDGTAFAEW"
     gene            complement(1496..1966)
                     /locus_tag="VI319_13635"
     CDS             complement(1496..1966)
                     /locus_tag="VI319_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY6864933.1"
                     /translation="MLRTGFTLIELVITVAIVALLASVALPVTELAVQRTKEQELRRT
                     LREIRDAIDAYKQASDEGRIKKSIDESGYPKKLELLVEGVDDQKSPKREKIYFLRRLP
                     RDPLNTDATLSADATWGKRSYQSPPDDPREGDDVFDVYSLSSGKGINGQPYKEW"
     gene            complement(1980..3938)
                     /locus_tag="VI319_13640"
     CDS             complement(1980..3938)
                     /locus_tag="VI319_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY6864934.1"
                     /translation="MKPIGLAWALAASLLVAACATPRSDAQRLIAEGREEEALALLQA
                     EAKDSNPARAEYYRVRDQLVSLWIVQADSVRFAQQYERAQALYERVLKWDPANARAKA
                     GVEQIETDKRQRAALASAERLLRNEKFAEAQDLVAPILAESPDNREAQRLQRLIDEHT
                     ARPASLTRQLRSSITKPVSLDLKDVPLRTVFDVLGRAANLNFLFDKDVRTDQKTTVVM
                     RDAQVEDIIRLILATNQLDQKILNETTTLIYPNTPQKQRDYQELIVRTFYLVNADARQ
                     VANLIRTILKTRDIYVDEKLNLLTIKDTPNVIRLAEKLVASHDLAEPEVMLEIEVLEV
                     SANKLYDLGLRYPSSVAWSLVGGSQNPVQQTTTATATTVTTNTAGTPGSLSLPEWLNR
                     NAGLVRLSVTDPLFVLSLRQQDGSTNLLANPRVRVKNKEKAKIHIGDRVPVLTTTAAV
                     AGGFVSQSVSYLDVGLKLDVEPLVYLNDDVGIKVGLEVSSITNTIQTQQSGSIVYQVG
                     TRNANTVLRLRDGETQILAGLINDEDRRSAQRVPGLGDFPIIGRLFSSTSSSKTKTEI
                     VLLITPHLMRQLDQPDARIVEFSAGTESSAGGGPLSGAPFIPPSPLPVAPQSAPAPAP
                     APAPAAPAAPQSGSTLVPFGGVAPSQQP"
     gene            complement(3935..4474)
                     /locus_tag="VI319_13645"
     CDS             complement(3935..4474)
                     /locus_tag="VI319_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864935.1"
                     /translation="MSRLHALLRRLGAAGVLGIGVLFFCVPFYFSTVRPVEAEAKMAR
                     AAADRLRTRSAYTPVAGDGRADELRRFYLLFPRLDELPAQLERIYGIARASRLELLQA
                     EYRLDRRGDGLVPYRITLPLRGTYPQVRDFVGATLQSVPNASIDALRFERKNPGDALL
                     DAQVRLTVYFRPNNNEGAQ"
     gene            complement(4471..4989)
                     /locus_tag="VI319_13650"
     CDS             complement(4471..4989)
                     /locus_tag="VI319_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864936.1"
                     /translation="MQRLELDYVAPPRRARWPGAVLLAVSLVVAAGVVERYRDARAEL
                     AAVEAAQGLVNLDRSSTKAIPRERLDEETKMVNDVVRQLGLPWARMVAAVERASTGDV
                     VVMQMQPDAQQRLLRLTAEAKSRQAMLQYVRRLGEERALADVFLVRDEVRTEQPGRPI
                     QFAVQATLRDVR"
     gene            complement(4992..5828)
                     /locus_tag="VI319_13655"
     CDS             complement(4992..5828)
                     /locus_tag="VI319_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864937.1"
                     /translation="MSLSFKAALARPRVVVGFSPAEVTLARIEGRLKPRLVAKRALGC
                     DPAAAGEPWQGAAGALAALAAEVRDVRCDVTVVLSNHFVRYALVPASDTLDSPEEESA
                     YARYCFSKVHGERARTWDVRLSGPDDGGLRVASAIDAALLEAIRACFPATGRPRLVSV
                     QPYLMAAFNRSDAGKGKHSGWLLLVEPQRACLACIDAGRWTAVRNVKGDYELPARWAE
                     LLERERYVAGAPEGDVIVHAGAAPSPAETGPWRFRLLAAAAPEGYSARDDGRFAMALC
                     AA"
     gene            complement(5812..>6289)
                     /locus_tag="VI319_13660"
     CDS             complement(5812..>6289)
                     /locus_tag="VI319_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY6864938.1"
                     /translation="ALTGHLVFTTVHANNVFDVIGRFMHMNVDLYGFVSALNAILAQR
                     LVRLVCTRCSEDDKPEPALLKESGLTPAEAASFRFRVGRGCRECRGSGYKGRKAIAEL
                     MVLNDELRELITARAPVRQLKEAARANGTRCLREAAFEAVRNGETTLQEINRVTFV"
BASE COUNT          890 a         2178 c         2236 g          985 t
ORIGIN      
        1 gacacgaaag acgagccggt gctgcacgcg cagggcgttc cgcccttcgg cgcgaacggc
       61 gtggggttca acaaagcctt cgacacgctg ttcgtcgcga acaccggtaa cgacacggtc
      121 gtcaccgtcc ccgcgtcggg cggcgtcgtc ggcaatgcca acgtgctcac caacagcatc
      181 aacggcgccg acggcttgat cctcgacgag caggacaaca tctgggtctg cgcgaaccag
      241 ggcgacgaga tcgtcgtgat cgacaagacc ggcaaggtca tctcgaagct cggcgacttc
      301 aacgggatcg accgcgacgg cgcgccgatc gggttcctgt tcccggcgag cctggtgcgg
      361 cacggcggct tcatctacgt gacgaacctc tcgctcgatc tgcgtcccgt ggtcggcgcg
      421 caggcggtcg acagccagtg ggccgcgcgg gtgacgacga acacgatctc gcgcatccgc
      481 gcgaagatcc tgcgcaatcc cgactgaggc acgccacggt ggacaagggc gccttcgggc
      541 gccttttttt ttcgccgaca cgtccgccag gtagtctcag cgcgcgggcg gcggcgccgg
      601 catcggcgcg atcggctgct gcgggttggc cgcgcccggc gcgggcgcgc ccggcacgat
      661 cgcctgctgc ttcgacggat cgtagacgaa cttccagtcc gagtacttcc cggcgccctc
      721 gaaggcgctg ttgcgcaggt cgaagccggc ggtcttcatc ggcgtgacgt cggagaggct
      781 gtacacgccc atcaccccgc cgtcgggcgc cttgacgatg ccccactcgg agctgcccgt
      841 caccgggtcg aaatagagac gtcgcagata gcgctggacg ttcggcttgc gcgggtcctt
      901 cagcaggtcc tcgagcgtgc gcggatactg ccgcggcccg gagagatagt agcgctcgat
      961 cgcgcgccgg tactggttcc ccgcgtacag gagttgcgat tccttgtcgc gcagcgccgc
     1021 ggtgtgccag acctcgccgg tgatggcgag gccgatgccg atgaacgcga ccgcgaagag
     1081 gatcgtgagg taagtgaacc cgcgctgatc ggcgggcatc gtcaccactc cgcgaacgcc
     1141 gtgccgtcgc tcgcctggcc cggcgctccg ctcttcacgt cgtacatgcc gctcttctgc
     1201 ggatcctgag gcggcacgac gacccaggtg tccttcttct cggtgatggg gtcgaccggg
     1261 acgctgcgca ggtacttctc ggagaccagc gcgtcgaggt tctcggggta cttgccgcgg
     1321 tcgccgtagt acttgtcgat cgcgtcgcgc atctggtgca ggtcctcgcg cagcaccgtc
     1381 tccttggagc ggtcgaggct cccgaaatag cgcggcagcg cgatcgtcag cagcagcgcg
     1441 atgatcgcga gaaccaccag cagctcgatc agcgtgaagc cgcctgtcct tcgcatcacc
     1501 attccttgta cggctggccg ttgatgccct tgccggaaga gagcgaatag acgtcgaaga
     1561 cgtcgtcgcc ttcgcgcgga tcgtcgggcg ggctctggta gctgcgcttg ccccaggtcg
     1621 cgtcggccga gagcgtcgca tcggtgttga gcgggtcgcg cggcaggcgc cgcaggaaat
     1681 agatcttctc gcgcttcggg ctcttctggt cgtcgacgcc ctcgaccagc agctcgagct
     1741 tcttcggata gcccgattcg tcgatcgact tcttgatccg gccctcgtcg gaggcctgct
     1801 tgtaggcgtc gatggcgtcg cggatctcgc gcagcgtgcg gcgcagctcc tgctccttgg
     1861 tccgctgcac ggcgagctcg gtcacgggca gcgcgaccga ggcgagcagc gccacgatcg
     1921 ccaccgtgat caccagctcg atgagggtga agcctgtcct gagcatcgtc gaagggcctt
     1981 cacggctgct gcgacggcgc gacgccgccg aaggggacga gcgtgctgcc cgactggggc
     2041 gccgccggcg cggccggcgc gggcgccgga gccggagcgg gcgcagactg cggcgcgacg
     2101 ggcagcggcg agggcgggat gaacggcgcg ccgctcagcg gtccgccgcc ggccgagctc
     2161 tcggtccccg cggagaactc gacgatgcgc gcatccggct ggtcgagctg gcgcatcagg
     2221 tgcggggtga tgagcaggac gatctcggtc ttggtcttgc tcgagctggt cgaggagaac
     2281 agccgcccga tgatggggaa gtcgccgagg ccgggcacgc gctgcgccga gcgccggtcc
     2341 tcgtcgttga tgaggccggc gaggatctgc gtctcgccgt cgcgcagccg cagaacggtg
     2401 ttggcgttgc gcgtgcccac ctgatagacg atcgagcccg actgctgcgt ctggatggtg
     2461 ttggtgatgc tgctcacctc gaggccgacc ttgatgccga cgtcgtcgtt cagatagacc
     2521 agcggctcga cgtcgagctt gaggcccacg tcgaggtagc tcaccgactg cgacacgaag
     2581 ccgcccgcga ccgccgccgt cgtcgtcagc accggcacgc ggtcgccgat atggatcttc
     2641 gccttctcct tgttcttgac gcgcacgcgc ggattggcga gcaggttggt gctgccgtcc
     2701 tgctgccgca gcgacagcac gaacagcggg tcggtgacgc tgaggcgcac cagccccgcg
     2761 ttgcggttca gccactcggg cagcgacagc gagcccggcg tcccggccgt gttcgtcgtg
     2821 accgtcgtcg cagtggcggt cgtggtttgc tggaccgggt tctgcgaccc gcccacgagg
     2881 ctccacgcga ccgagctcgg gtagcgcagc ccgaggtcgt agagcttgtt cgcgctcacc
     2941 tcgagcacct cgatctcgag catgacctcg ggctcggcca ggtcgtgcga ggcgaccagc
     3001 ttctccgcga gccggatcac gttcggcgtg tccttgatcg tgagcaggtt cagcttctcg
     3061 tcgacgtaga tgtcgcgcgt cttgagaatg gtgcggatga ggttcgcgac ctggcgggcg
     3121 tcggcgttga ccaggtagaa ggtgcggacg atcagctcct ggtagtcgcg ctgcttctgc
     3181 ggcgtgttcg ggtagatcag cgtggtcgtc tcgttgagga tcttctggtc gagctggttg
     3241 gtcgcgagga tcagccggat gatgtcctcg acctgcgcgt cgcgcatcac caccgtggtc
     3301 ttctggtcgg tgcgcacgtc cttgtcgaac aggaagttga ggttcgccgc gcggccgagc
     3361 acgtcgaaca cggtgcgcag cggcacgtcc ttcaggtcga gcgacacggg cttggtgatc
     3421 gagctgcgca gctggcgcgt gagcgacgcc gggcgcgcgg tgtgctcgtc gatcaggcgc
     3481 tgcaggcgct gcgcctcgcg gttgtcgggg ctctcggcca ggatgggcgc gacgagatcc
     3541 tgcgcctcgg cgaacttctc gttgcgcagc agccgctcgg cgctggcgag ggccgcgcgc
     3601 tggcgcttgt cggtctcgat ctgctcgacc ccggccttgg ctcgggcgtt cgcgggatcc
     3661 cacttgagca cccgctcgta cagcgcttgc gcgcgctcgt actgctgggc gaagcgcacg
     3721 ctgtccgcct gaacgatcca cagcgagacc agctgatcgc gcacgcggta gtactcggcg
     3781 cgggccgggt tgctgtcctt ggcctccgcc tgcagcaggg cgagcgcctc ctcctcgcgg
     3841 ccttccgcga tgaggcgctg cgcgtcgctg cgcggcgtcg cgcaggcggc gacgaggagc
     3901 gaggcggcga gggcccaggc gagtccgatc ggtttcattg cgcaccttcg ttgttgttgg
     3961 gacggaagta caccgtgagg cggacctggg cgtcgagcag cgcgtcgccc gggttcttgc
     4021 gctcgaaccg cagcgcgtcg atggaggcgt tcggcaccga ctgcagcgtc gcgccgacga
     4081 agtcgcgcac ctgcggatag gtgccccgca gcggcagcgt gatgcggtac ggcaccaggc
     4141 cgtcgccgcg gcggtccagg cggtactcgg cctggagcag ctcgagccgc gacgcgcgcg
     4201 cgatgccgta aatgcgctcg agctgcgccg gcagctcgtc caggcgcggg aacagcaggt
     4261 agaagcggcg cagctcgtcg gcgcgcccgt cgcccgccac cggcgtgtac gcgctgcgcg
     4321 tgcgcaggcg atccgcggcg gcgcgcgcca tcttcgcctc ggcctcgacc ggacgcaccg
     4381 tcgagaaata gaacggcacg cagaagaaga gcacgccgat cccgagcacg cccgcggcgc
     4441 cgagacggcg aaggagcgcg tgcaggcggc tcatcggacg tccctcagcg tcgcctggac
     4501 cgcgaactgg atcgggcggc ccggctgctc ggtgcgcacc tcgtcgcgca ccaggaagac
     4561 gtcggcgagc gcgcgctcct cgccgaggcg gcgcacgtac tgcagcatcg cctggcggct
     4621 tttcgcctcc gcggtgagac gcagcagccg ctgctgcgcg tcgggctgca tctgcatcac
     4681 gaccacgtcg ccggtcgagg cgcgctcgac cgcggcgacc atccgcgccc acggcagccc
     4741 cagctggcgc accacgtcgt tcaccatctt ggtctcttcg tcgaggcgct cgcgcgggat
     4801 cgccttggtc gaggaacggt cgaggttgac gaggccctgc gccgcctcga ccgcggcgag
     4861 ctcggcgcgc gcgtcgcggt agcgctcgac cacgcccgcc gccaccacca gggacaccgc
     4921 gagcagcacc gcgcccggcc agcgcgcgcg cctcggcggg gcgacgtaat cgagctcgag
     4981 ccgctgcatg ctcaggcggc gcagagcgcc atcgcgaagc gcccgtcgtc gcgcgccgag
     5041 tatccttcgg gcgcggccgc ggcgaggagg cggaagcgcc acgggcccgt ttcggccggc
     5101 gacggcgcgg cgcccgcgtg cacgatgacg tcgccttcgg gcgcgcccgc cacgtagcgc
     5161 tcgcgctcga gcagctcggc ccagcgcgcg ggcagctcgt aatcgccctt gacgtttcgg
     5221 accgcggtcc agcggccggc gtcgatgcag gcgaggcacg cgcgctgcgg ctcgaccagc
     5281 agcagccagc ccgagtgctt gcccttgccc gcgtcggagc ggttgaacgc ggccatgagg
     5341 taaggctgca ccgacacgag ccgcggcctg cccgtcgccg ggaagcaggc gcggatcgcc
     5401 tcgagaagcg cggcgtcgat cgcgctcgcg acgcgcaacc cgccgtcgtc ggggccggag
     5461 agccgcacgt cccaggtcct ggcgcgctcg ccatgcacct tggagaagca gtagcgcgcg
     5521 tacgccgatt cttcctcggg gctgtcgagc gtgtcgctcg ccgggaccag cgcatagcgc
     5581 acgaagtggt tcgagaggac gaccgtcacg tcgcaccgga cgtcgcggac ctccgccgcg
     5641 agcgccgcga gcgcgcccgc cgcgccttgc cagggctcgc ccgccgcggc gggatcgcag
     5701 ccgagcgcgc gcttggcgac cagccgcggc ttcagccggc cctcgatccg ggcgagcgtg
     5761 acctcggccg gcgagaaccc cacgacgacg cgcgggcgcg cgagcgcggc cttaaacgaa
     5821 agtgacacgg ttgatctcct gcagcgtcgt ctcgccgttg cgcacggcct cgaacgccgc
     5881 ctcgcgcaga cagcgcgtgc cgttcgcgcg agccgcctcc ttcagctggc gcaccggggc
     5941 gcgcgccgtg atcagctcgc gcagctcgtc gttcagcacc atcagctccg cgatcgcctt
     6001 gcgccccttg tagcccgagc cccggcactc gcggcacccg cggcccacgc ggaagcggaa
     6061 cgaggcggcc tcggccggcg tcagcccgga ctccttgagc agcgcgggct cgggcttgtc
     6121 gtcctcgctg cagcgcgtgc acacgagccg caccaggcgc tgcgcgagga tcgcgttcag
     6181 cgccgacacg aagccgtaca ggtcgacgtt catgtgcatg aagcgcccga tcacgtcgaa
     6241 cacgttgttc gcgtgcaccg tcgtgaacac caggtggccc gtcagcgcc
//
LOCUS       DATDMW010000326         4113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_345206, whole genome shotgun sequence.
ACCESSION   DATDMW010000326 DATDMW010000000
VERSION     DATDMW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345206"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VI319_13665"
     CDS             complement(<1..253)
                     /locus_tag="VI319_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY6864939.1"
                     /translation="MAKRRSAGILLWRRRGGVLEVMLVHPGGPLWSKKDEGAWSIPKG
                     EFEPGESAEAVAMREFREETGFGCEGDLIALEPCRQPGGK"
     gene            385..747
                     /locus_tag="VI319_13670"
     CDS             385..747
                     /locus_tag="VI319_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864940.1"
                     /translation="MPRPSLRALAAVVAALLASSAGAAVGSPPRIPNEQACRYLADYA
                     LVARALAEERTLSTSLADSILNRIYIVNHPVITDVEGRIRLIARGDKRTAQEFSLAFL
                     KECVGHDGDVTAFLGVSL"
     gene            complement(744..2228)
                     /locus_tag="VI319_13675"
     CDS             complement(744..2228)
                     /locus_tag="VI319_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY6864941.1"
                     /translation="MKPDDLHGAPPEPTVSREEFLNLFASVFLPMFMAAVDQTLLATA
                     TPAIAASLGGLRDTSWIAVAYLLASAVVVPVYGRLGDLRGRRNVLLGALALFTAGSLA
                     CGFAQSLPQLVAARVVQGAGGGGLMMLSQALIAELVPPRERARFQGYFAIVFTSASVG
                     GPVLGGLVVSSLSWRWLFFANLPLALFAAWRLLRLPAGEKHPGRSGLRDPAGIGLFFV
                     GMSTALFWLTAVGHHFSWRSPESALLLAVAATSLALLAWHQLRHPAPFLPVELMRQRA
                     IAFSVLTTALFASCMFAMVFFLPIYLQLGHHFSPTASGLLLLPLTAGMVTGSITTGRI
                     IARTGRPRLLPVFGLSVSSVALAALGIVPSNTFLVALLGFVTGLGYGTVMPVNQVVAQ
                     TAAGRARLGAVTATISLARSVGAATGAALFGALIYAMMPDVDLRALVMSAAGGEGLAQ
                     TLLRAFHRAFLGAAVVAALAAFAASRVPHVPLWGGESNAGAGGI"
     gene            2344..3537
                     /locus_tag="VI319_13680"
     CDS             2344..3537
                     /locus_tag="VI319_13680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6864942.1"
                     /translation="MSPTVTTPPRALDSRLMRLFAVTMFAAAGSIHFQTAMLGAIARE
                     FGASPGEVGWVPTMTFAGFVSGIVFIVPLGDRADKRRLLLVQHVALIASLLAMAMAPS
                     LAALAALSFVMAAVSCFSQNILPLVSELARSEERGRAVATILTALFLGILFARVGGGV
                     VASTLGWRWMYVISAAMLAALGPALARRVPPSPPKTALPYRVLIGSLVHLVRTNAELR
                     RASATQYLLGICYGGFWATLAPMLISLHGLGPAQIGLMAIPGSAGIFIARPAGRWMDR
                     RGVVPVVTTGCGLVLAAFVAFGFAAWWVGAVVAGAVLLDSGLRSAMVANQTLINSQVA
                     DARSRFNTIFAAHVWGGNATGALLASMALAHAGWLAVCAIGVASAACAVALQWRARRV
                     AGEAP"
     gene            complement(3557..3970)
                     /locus_tag="VI319_13685"
     CDS             complement(3557..3970)
                     /locus_tag="VI319_13685"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6864943.1"
                     /translation="MTAEITIVNPDGLGKPLGQYSQIARVKNASEIVFIAGQLATDRA
                     GGVVGADDFDAQCVQVFANIGAALASVGGGWKNVAQFTTYLVHSQDVPKFMAFRKREF
                     PRMFADGAYPPNTLLMVDRLVQEPFLVEVQAIAAL"
BASE COUNT          594 a         1540 c         1421 g          558 t
ORIGIN      
        1 gcttgccgcc cggctgccgg cacggctcga gcgcgatcag atcgccctcg cacccgaaac
       61 ccgtctcttc ccggaactcg cgcatcgcca ccgcctccgc actctccccg ggctcgaact
      121 cccccttggg aatcgaccac gcaccttcgt ccttcttgga ccacagcggg ccgccggggt
      181 gcacgagcat cacctcgagc acgccgccgc ggcgccgcca gaggaggatg cccgcgcttc
      241 gccgctttgc catggcccgt attacaacat cggacgcgcc gcggtccaaa cggaccgcgc
      301 ggcgcgccgc cggccgtcac aaaggcggcg gcctcgcgcc ttcaagatgg cctccaccgc
      361 ccccaccctc gatcggacat caccatgccc agaccctccc tgcgagccct cgccgcagtc
      421 gtcgccgccc tgctcgcctc gtccgcgggc gccgccgtcg gttcgccgcc ccgcatcccg
      481 aacgagcagg cctgccggta cctcgccgac tacgcgctcg tcgcccgcgc gctcgccgag
      541 gaacggacgc tctcgacgtc gctggccgac tcgatcctca accgcatcta catcgtcaat
      601 catccggtca tcaccgacgt cgaagggcgg atccgcctga tcgcgcgcgg cgacaagcgc
      661 acggcgcagg aattctcgct cgcgttcctg aaggagtgcg tcgggcacga cggcgacgtg
      721 accgccttcc tcggcgtcag cctctagatc cctccggccc cggcgttgct ctcgccgccc
      781 cacagcggga cgtgcggcac gcggctcgcc gcgaacgccg cgagcgcggc cacgaccgcc
      841 gcgcccagga acgcgcggtg gaacgcgcgc agcagcgtct gcgcgaggcc ctcgccgccc
      901 gccgccgaca tgaccagcgc gcgcagatcg acgtcgggca tcatcgcgta gatcagggcc
      961 ccgaagagcg cggcgcccgt cgccgccccg accgagcgcg cgagcgagat cgtcgccgtc
     1021 accgcgccca accgcgcccg acccgccgcg gtctgcgcga ccacttggtt gaccggcatc
     1081 accgtgccgt acccgaggcc ggtgacgaat ccgagcagcg cgaccaggaa tgtgttcgag
     1141 ggcacgatgc cgagcgcggc cagcgcgacg ctcgagaccg acaagccgaa gacgggcagc
     1201 agccgcggcc ggccggtgcg cgcgatgatg cggccggtgg tgatcgatcc cgtcaccatt
     1261 cccgccgtga gcggcagcag cagcaggccc gaggcggtcg gactgaagtg gtgcccgagc
     1321 tgcagataga tcggcaggaa gaacaccatc gcgaacatgc acgaggcgaa gagcgccgtg
     1381 gtcagcaccg agaacgcgat cgcgcgctga cgcatcagct cgaccggcag gaacggcgcg
     1441 gggtggcgca gctgatgcca cgcgagcagc gcgagcgacg tcgcggcgac cgcgagcagc
     1501 agcgcgctct ccggcgaccg ccacgagaaa tgatgcccga cggcggtgag ccagaaaagc
     1561 gccgtgctca tcccgacgaa gaaaagcccg atacccgcgg ggtcgcgcag tcccgaccgg
     1621 ccgggatgct tctccccggc cggcagccgc agcagccgcc acgcggcgaa gagggcgagc
     1681 ggcaggttgg cgaagaacag ccagcgccag ctgagcgacg acaccacgag gccgccgagc
     1741 accggtccgc cgacgctggc gctggtgaat acgatcgcga agtagccctg gaagcgcgcg
     1801 cgctcgcgcg gcggcacgag ctccgcgatc agcgcctgcg agagcatcat cagcccgccg
     1861 ccgcccgcgc cctgcacgac gcgcgccgcg acgagctgcg gcagcgactg cgcgaacccg
     1921 cacgcgagcg agcccgccgt gaacagcgca agcgcgccga gcagcacgtt gcgccgcccg
     1981 cgcaggtcgc cgaggcggcc gtacacgggc acgaccaccg cggaggcgag gaggtacgcg
     2041 accgcgatcc acgacgtgtc gcgcaggccg ccgaggctcg ccgcgatcgc gggggtcgcc
     2101 gtggcgagca gcgtctggtc gaccgccgcc atgaacatcg gcaggaatac cgaggcgaag
     2161 agattcagga actcttcgcg cgaaacggtg ggctcgggcg gagcgccgtg gaggtcgtcg
     2221 ggcttcatgg cttcctgtac gaccgggacg cctcggacgc gttccggcta gaccgcgtgg
     2281 cccgcgccct ggtagcccct catggcttta aactctagca cgccgcccgc cgatccgaga
     2341 acgatgtccc cgaccgtcac cacgccgccg cgcgcgctcg actcccgcct gatgcggctc
     2401 ttcgccgtca ccatgttcgc cgccgcgggc agcatccact tccagacggc gatgctcggc
     2461 gcgatcgcgc gcgagttcgg ggcgagcccg ggcgaggtcg gctgggtccc gacgatgacg
     2521 ttcgccggct tcgtctcggg catcgtcttc atcgtcccgc tcggcgaccg cgccgacaag
     2581 cgccgcctgc tgctcgtgca gcacgtcgcg ctgatcgcct cgctgctggc gatggcgatg
     2641 gcgccgtcgc tcgcggcgct cgcggccctc tccttcgtca tggccgcggt gtcgtgcttc
     2701 agccagaaca tcctgccgct ggtctcggag ctcgcgcggt ccgaggagcg cgggcgcgcg
     2761 gtcgcgacca tcctcacggc gctgttcctc ggcatcctgt tcgcgcgcgt cggcggcggc
     2821 gtcgtcgcgt cgacgctcgg ctggcgctgg atgtacgtga tctcggcggc gatgctcgcc
     2881 gcgctcgggc ccgcgctcgc gcggcgcgtg ccaccgtcgc cgccgaagac ggcgctgccg
     2941 tatcgcgtcc tgatcgggtc gctcgttcac ctcgtgcgca ccaacgccga gctgcggcgc
     3001 gcgtcggcga cgcagtacct gctcgggatc tgctacggcg gattctgggc gacgctcgcg
     3061 ccgatgctga tctcgctgca cgggctcggg cccgcgcaga tcgggctgat ggcgattccc
     3121 ggatccgccg ggatcttcat cgcgcgcccc gcgggccgct ggatggaccg gcgcggcgtg
     3181 gtgcccgtgg tgacgacggg ctgcggcctg gtgctcgcgg ccttcgtggc cttcggattc
     3241 gccgcgtggt gggtcggcgc cgtggtcgca ggcgcggtcc tgctcgacag cggcctgcgc
     3301 tcggcgatgg tcgcgaacca gacgctgatc aactcgcagg tcgccgacgc gcgcagccgc
     3361 ttcaacacca tcttcgccgc gcacgtctgg ggcgggaacg ccaccggcgc cttgctcgcg
     3421 agcatggcgc tcgcgcacgc ggggtggctc gcggtgtgcg cgatcggagt cgcctccgcc
     3481 gcgtgcgcgg tggcgctgca gtggcgcgcg cggcgcgtgg ccggcgaggc gccctagctc
     3541 cgcgccggac gccgttctac agcgcggcga tcgcctgcac ttcgaccagg aacggctcct
     3601 gcaccaggcg gtcgaccatc agcagggtgt taggcgggta ggcgccgtcg gcgaacatgc
     3661 gcgggaactc gcgcttgcgg aaggccatga acttcggcac gtcctgcgag tgcacgaggt
     3721 aggtggtgaa ctgcgcgacg ttcttccagc cgccgccgac cgaggcgagc gccgcgccga
     3781 tgttggcgaa gacctgcacg cactgcgcgt cgaagtcgtc ggcgccgacg acgccgccgg
     3841 cgcggtcggt ggcgagctgg ccggcgatga acacgatctc cgaggcattc ttcacccgag
     3901 cgatctgcga gtactgcccg agcggcttcc cgagcccgtc gggattgacg atggtgatct
     3961 cggcggtcat ctccacctcc tcgcgccggt tcagcgcttc ttcagcaaat gcccccactt
     4021 cttcgcgatc ttcgcgtgca gctcggggct ggagcccacg gtcggcggaa agtcggccat
     4081 cgtctcgaag ggcttgcacg cgacgacgag cgc
//
LOCUS       DATDMW010000327         7364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_966366, whole genome shotgun sequence.
ACCESSION   DATDMW010000327 DATDMW010000000
VERSION     DATDMW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966366"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VI319_13690"
     CDS             complement(<1..445)
                     /locus_tag="VI319_13690"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY6864944.1"
                     /translation="MLAIAAVLLAPAASARDGDETDHQGRSAAVTMTGTVVSIGREGF
                     GLDTGSGIVRVEIERTTVLTRNGQPAGAADLAVGQSVTVKGRLERNELEARTVDIHSG
                     VTLSGVIQSLAPPALNVSGTPVVTTAATVFTRNGAPAAFADLRIGD"
     gene            585..2510
                     /locus_tag="VI319_13695"
     CDS             585..2510
                     /locus_tag="VI319_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6864945.1"
                     /translation="MPTAIAPRVLSLIPPMTQLNTPYPSTAYLTGFLRSRGVDAHQED
                     LALELVLRLFSREGLATLRDAAEAKAAAKRSAPVRHFLREYARYADTVDAAIAYLQGR
                     DPTLAHRIASRAFLPEGARFAPLDVYVAKAGEDPLAWAFGALGVADRARHIATLYLED
                     LADALRDAADERFEFVRYAERLAANQPSFDLLARALAAEPTLVDRTLEALTLEALGRH
                     RAAVVLLSVPFPGSVYAALRIAQAVKRHNPDVVTVLGGGYVNTELRELKEPRLFDAID
                     YVTLDAGERPLLALLEHVQGRRSSRERLVRTFYREDGRVRYASFPEPDVPFEETGTPT
                     WDGLPLGRYLSLLDMLNPMHRLWSDGRWNKLTVAQGCYWKKCTFCDTSLDYISRYEAA
                     SATTLVDRIEAIVAETGQTGFHFVDEAAPPKVLKALAAELQARGTSISWWGNVRFEKT
                     FTPELCRKLAASGCIAISGGLEVASDRLLKLIDKGVTVEQVARVTRAFADAGVLVHAY
                     LMYGFPTQTVQDTVDALEYVRQLFANGCVHSGYFHRFTCTVHSPVGKHPERFGVKLRP
                     LPEGAFAKNDIGFVDPTGVDHDRLGRALAKALYNFMHGVGLDEDVRVWFEDEAGGQRV
                     PKATVGRRTIEKALAAV"
     gene            complement(2831..3100)
                     /locus_tag="VI319_13700"
     CDS             complement(2831..3100)
                     /locus_tag="VI319_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864946.1"
                     /translation="MARQVSEWRLVVNLIGSAVIGVPAWIVAALMAPVFFQPWPDTGD
                     PEVRKGLGVAILGAVVCALVGVACLVAFLRTVKALRHPGDSAADR"
     gene            3232..5178
                     /locus_tag="VI319_13705"
     CDS             3232..5178
                     /locus_tag="VI319_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864947.1"
                     /translation="MERSALVLRLLVLLGLACALAPALGQQRPSPFAAPPAQQQASPG
                     APFSPARAAAASVILGGDDHAQVMRRLRVLRQYRVADLRVQPRLALGAMRVNVAPMLN
                     APAATANIAQKLRSLPRHVEVRAEETTVAEIEQGLVMHSLLRYRMRGVACRDADARAQ
                     LAAAGASCFTAQPAGGDAPAYSRRGDPRYVADPARRARALAEYQQKTAAAQALNAKRI
                     AALRKALSDPQRRAAHEAKLGAAEFQRVSRLTDAQLEEEVVNSAVVTIEEIVFLSRSA
                     ALARSPRIAALGIAQHQPSARERFAARQAPPPQSPSKAQPPRAAQAEAHRTQPLGTYI
                     YLTGFTLGSHHEWRRRVSVTIDWCLGLFDDCEETYYLEPHAGFEYGFGLRFPIQATLN
                     YTFDWRATQPPTGHAAVKIDYAPVMGTPKMFQDAGLAPEKIFDGKEFVCEAGADAGLA
                     YRVPILGSDDFDKSIGFDLTTGLPAPFTNGRFMPPAPGTNIAAKDPILLEDMDFLGGL
                     ANYGVAGAKVLPGVQVGLRSNGLTFTLIDKVAQKSSSVAQTGTTVPLGVRNDRVMSSE
                     FGLADPVYDLTLQLTPGIDGRLFIYIGVWSHDWDWWIPFPALEIDLPPGGGARFSCHD
                     GTQCERDFSEPGIPVKFPRLPPSL"
     gene            5200..5820
                     /locus_tag="VI319_13710"
     CDS             5200..5820
                     /locus_tag="VI319_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864948.1"
                     /translation="MEAFFAAYVTAWGLACAAAGAIVARDPSGFPILAPEYRRFLGVR
                     WKVVTFVLAAAGLTVVAPYTGDPTWDYFDAAFQSVLTFLGAPWAVGTLYLFIRRAVRL
                     KQAFVAACVWMFSASWSYDVYILARDGRYPDTWLVNIAASSVLYMAAGLLWNLDWRPG
                     RGVTFSFLEPGWPGVPPAPQFARLMGYALPFMLIAAASVAYFLATA"
     gene            5820..6122
                     /locus_tag="VI319_13715"
     CDS             5820..6122
                     /locus_tag="VI319_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864949.1"
                     /translation="MAVPAPECPRCKVALTDAESLASICGDVFGDEYIDTFYRCAHCA
                     TYCRESFRDRFSGPSSARMEPLEPRAAEALIELVRRCPSPAHKHCRCEAHREYFRI"
     gene            complement(6119..6601)
                     /locus_tag="VI319_13720"
     CDS             complement(6119..6601)
                     /locus_tag="VI319_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864950.1"
                     /translation="MLLTLLVLALAACATPYQPRGLLGGYAETQLGENIFRVSFTGNG
                     DTSVERAEDFALLRSAEVALEHGFTHFVVIAEKDQSQYVTHREPTRSVSSQTSTTSRD
                     TTRTKTTTTTSGGQTTVVRQPGTVNLIACFNGKPDVPEMVFDAQFLVNSLTQKYEIVR
                     "
     gene            complement(6655..>7364)
                     /locus_tag="VI319_13725"
     CDS             complement(6655..>7364)
                     /locus_tag="VI319_13725"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HEY6864951.1"
                     /translation="LGRFELVRDGVPVKSGGKAQQRVLDLLKAIVAFGPRGAASDTIA
                     SALWPASDGDAAQTALDVTLHRLRKLLGLEGAVVVADGRIALNPRCCWVDAFAFEQAL
                     DACERDGGELAEAERALGLYAGHFLQGDEETPWLLPRRERLRSKFLRCVERCGATREE
                     RGEHPAAVELYRRALEVDPLAEGVYRNLMAAYLAAGRRAEALEAYRRCRHMLSVVLGV
                     PPSAETEAMRARVSANR"
BASE COUNT         1026 a         2669 c         2568 g         1101 t
ORIGIN      
        1 ggtcgccgat gcgcagatcg gcgaacgcgg ccggcgcccc gttgcgcgtg aacaccgtcg
       61 cagcggtcgt gaccaccggc gtgccggaaa cgttcagcgc cggcggcgcg agcgactgga
      121 tgacgcccga aagcgtgacg ccggagtgga tgtcgaccgt cctcgcctcg agctcgttcc
      181 gctcgaggcg gcctttgacc gtcacgctct ggccgacggc gagatcggcc gcgcccgccg
      241 gctggccgtt cctggtcagc accgtggtcc gctcgatctc gacccgcacg atgccgctgc
      301 cggtatcgag gccgaagccc tcgcggccga tcgagaccac cgttccggtc atcgttacgg
      361 ccgcggaccg gccctggtga tcggtttcgt cgccgtcccg cgccgacgcc gccggcgcca
      421 gcagcaccgc ggcaattgcg agcaagcgga tgaatgttgt tgtcacgcgc gcctcccgga
      481 agtcgaggcc gtcaccctag cggcggggca cttacggatt gcttaccgat tcacgacctc
      541 ccgccagcgc ttctccacgg ccccacatgc gctatcctcg cggcatgccc accgcgatcg
      601 cgccgcgcgt gctgagcctc attccgccga tgacgcagct caacacgcct tatccgtcca
      661 ccgcgtatct caccgggttc ctgcgctcgc gcggggtcga tgcgcaccag gaggacctcg
      721 cgctcgagct ggtgctgagg ctgttctcgc gcgaagggct cgcgacgctg cgcgacgcgg
      781 ccgaggcgaa ggccgcggcg aagcgctcgg cgccggtgcg ccacttcctg cgcgagtacg
      841 cgcgctacgc cgacaccgtc gacgcggcga tcgcgtacct gcaggggcga gacccgacgc
      901 tcgcgcaccg gatcgcctcg cgcgcgttcc tccccgaagg cgcgcgcttc gccccgctcg
      961 acgtctacgt ggcgaaggcc ggcgaggatc cgctggcgtg ggccttcggc gcgctcggcg
     1021 tcgccgaccg cgcgcggcac atcgccacgc tctatctcga ggatctcgcc gacgcgctgc
     1081 gcgacgcggc cgacgagcgc ttcgagttcg tgcgctacgc cgagcgcctc gccgcgaacc
     1141 agccgagctt cgatctgctc gcccgcgcgc tcgcggccga gccgacgctc gtcgaccgca
     1201 cgctcgaggc gctgacgctc gaggcgctcg ggcgccaccg ggccgcggtg gtgctgctgt
     1261 cggtgccgtt tcccggctcg gtctacgcgg cgctgcgcat cgcgcaggct gtgaagcggc
     1321 acaacccgga cgtcgtcacc gtgctcgggg gcggctacgt caacaccgag ctgcgcgagc
     1381 tgaaggagcc gcggctcttc gatgccatcg actacgtgac gctcgacgcg ggcgagcggc
     1441 cgctgctcgc gctgctcgag cacgtgcagg gtaggcgttc gagtcgcgag cgcctggtgc
     1501 gcaccttcta ccgcgaggac ggccgggtgc gctatgcgag cttcccggag cccgacgtcc
     1561 cgttcgagga gaccggcacg ccgacctggg acgggctgcc gctcggccgc tatctctcgc
     1621 tgctcgacat gctgaacccg atgcaccggc tctggagcga cgggcgctgg aacaagctca
     1681 ccgtcgcgca agggtgctac tggaagaagt gcaccttctg cgacaccagc ctggactaca
     1741 tctcgcgcta cgaggcggcg tcggcgacga cgctcgtcga ccgcatcgag gcgatcgtcg
     1801 ccgagaccgg ccagaccggc ttccacttcg tcgacgaggc cgcgccgccg aaagtgctga
     1861 aggcgctcgc cgccgagctg caggcgcgcg gcacgtcgat ctcctggtgg ggcaacgtgc
     1921 gcttcgagaa gactttcacg cccgagctgt gccgcaagct cgccgcgagc ggctgcatcg
     1981 cgatctcggg cgggctcgag gtcgcctccg accggctgct caagctcatc gacaagggcg
     2041 tcaccgtcga gcaggttgcg cgcgtcacgc gcgccttcgc cgacgcgggc gtgctcgtgc
     2101 acgcctacct catgtacggc ttcccgacgc agaccgtgca ggacaccgtg gacgcgctcg
     2161 aatatgtgcg ccagctgttc gccaacggct gcgtccacag cgggtatttc caccgcttca
     2221 cctgcaccgt gcattcaccc gtgggaaagc accccgagcg attcggcgtg aagctgcggc
     2281 cgctgcccga aggcgccttc gcgaagaacg acatcggatt cgtcgatccg accggcgtcg
     2341 accacgaccg cctcggccgc gcgctcgcaa aagcgctcta caacttcatg cacggcgtcg
     2401 gcctcgacga ggacgtgcgc gtctggttcg aggacgaggc cgggggacag cgcgtgccga
     2461 aggcgacggt cggtcggcgg acgatcgaga aggcgctggc ggcggtgtag cgccggacag
     2521 gccggggggt caatggcccc gcaactgatc ggacaggaaa tagctgtcgc acgtcctgca
     2581 ccggaggagc cggaccgtgc gcggcacgtc ggccggcgtc cggtcctcga tatccgtcga
     2641 tccgcaacga aagcacaccg gcgcaggcgg gtcctgcggc cggtccgcgg gtgcatcctc
     2701 cacggtccaa gcctcctcct caggagtcgg gaccgagttt cttttctttg gcccgcgggc
     2761 cgtttgatgc agatcaaagg tcggtcggcg cggttccggc ctaagggttg cccccgggct
     2821 taccgatcgc ttaccgatcg gccgcggagt cgcccgggtg gcgcagcgcc ttcaccgtcc
     2881 gcaggaacgc gaccaggcag gcgacgccga ccagcgcgca gacgaccgcc ccgaggatgg
     2941 cgacgcccag gcctttgcgc acctcgggat cgcccgtgtc gggccacggc tggaagaaca
     3001 cgggcgccat gagcgccgcg acgatccacg cgggcacgcc gatgacggcg gaaccgatca
     3061 ggttcaccac gagccgccat tccgagacct gccgcgccat gagttcctcc cggccgcaaa
     3121 tgtaatacgg atgcgggccg cgcgtcagcg cgccccgccc gccgacgcgt aagtcatcgg
     3181 taagccgcag gatgattagc tgcgcggatt tcgtgaggag gactcgacgc gatggaacgc
     3241 tccgctctcg tcttgcgact gctcgtgctg ctcgggctgg cgtgcgccct ggcgcccgcc
     3301 ctcggccagc agcgcccgtc gccgttcgcc gcgccgccgg cgcagcagca ggcgtccccg
     3361 ggcgcgccct tctcgccggc gcgcgctgcg gccgcgtcgg tcatcctggg cggcgacgat
     3421 cacgcgcagg tcatgcgcag gctgcgggtg ctgcggcaat atcgcgtcgc cgacctgcgc
     3481 gtccagccgc gcctcgcgct cggcgcgatg cgggtcaacg tggcgcccat gctgaatgcc
     3541 ccggccgcca ccgcgaacat cgcgcagaag ctgcgctcgc tgccgcgcca cgtcgaggtg
     3601 agagcggagg agacgacggt ggcggagatc gagcagggcc tggtgatgca ttcgctgctg
     3661 cgctatcgca tgcggggcgt cgcctgccgc gacgcggacg cgcgcgcgca gctcgcggcc
     3721 gcgggggcgt cttgcttcac ggcgcagccg gcgggcggcg acgctccggc ctactcgcgg
     3781 cgcggcgacc cgcgctacgt cgcggacccg gcgcggcgcg cgcgcgctct ggcggaatat
     3841 cagcagaaga ccgcggccgc gcaggcgctg aacgcgaagc gcatcgccgc gctgcgcaag
     3901 gcgctctccg atccgcagcg gcgcgccgcg cacgaagcga agctcggcgc ggccgaattt
     3961 cagcgcgtga gccgcctgac cgacgcgcag ctcgaggagg aggtggtcaa ctccgccgtc
     4021 gtgacgatcg aggagatcgt gttcctctcg cgctcggccg cgctcgctcg ctcgccgcgg
     4081 atcgcggcgc tcggcatcgc gcagcaccag ccgtcggccc gcgagcgctt cgcggcgcgg
     4141 caggcgccgc cgccgcagtc gccctcgaaa gcgcagccgc cgcgcgcggc gcaggccgag
     4201 gcgcaccgga cgcagcccct cggcacgtac atctacctca ccggcttcac gctcggctcg
     4261 catcacgaat ggcgccggcg cgtgtcggtc accatcgact ggtgcctcgg cctgttcgac
     4321 gactgcgagg agacctacta cctcgagccg cacgcgggct tcgaatacgg gttcgggctg
     4381 cgcttcccga tccaggcgac gctcaactac acgttcgact ggcgcgcgac ccagccgccg
     4441 acggggcatg ccgccgtgaa aatcgactac gcgcccgtca tgggcacgcc gaagatgttc
     4501 caggacgccg gcctggcgcc ggaaaagatc ttcgacggca aggagttcgt gtgcgaggcg
     4561 ggcgccgatg cgggcctggc gtaccgggtg ccgatcctcg gcagcgacga tttcgacaag
     4621 tccatcggct tcgacctgac gaccgggctg ccggcgccgt tcaccaacgg caggttcatg
     4681 ccgcccgcgc cgggcaccaa catcgccgcg aaggacccga tcctcctcga ggacatggac
     4741 ttcctcggcg ggctcgcgaa ctacggcgtg gcgggcgcga aggtcctccc cggcgtccag
     4801 gtcggcctgc gctcgaacgg gctgacattc acgctgatcg acaaggtggc gcagaagagc
     4861 tcgagcgtcg cgcagaccgg cacgacggtt ccgctcggcg taaggaacga tcgcgtcatg
     4921 tcgagcgagt tcggcctcgc cgacccggtg tacgacctca cgctgcagct cacgcccggc
     4981 atcgacgggc gcctgttcat ctacatcggc gtgtggtcgc acgattggga ctggtggatc
     5041 ccgttcccgg cgctcgagat cgacctcccg cccggcggcg gcgcgcgctt cagctgccac
     5101 gatggcacgc agtgcgagcg cgacttcagc gagccgggta tccccgtcaa gttccccagg
     5161 ctgccgcctt cgctatagtg gcggaataac gggaggcgca tggaggcgtt cttcgccgcg
     5221 tacgtgaccg cctggggcct ggcgtgcgcc gcggcgggag cgatcgtcgc gcgcgacccg
     5281 tcgggcttcc cgatcctcgc gccggagtac cggcggttcc tcggcgtgcg ctggaaggtc
     5341 gtcaccttcg tgctcgccgc ggccgggctc acggtcgtcg ccccttatac gggcgacccg
     5401 acctgggact acttcgacgc ggcattccag tcggtgctga ccttcctcgg ggcgccgtgg
     5461 gccgtaggaa cgctctatct cttcatacgg cgcgcggtgc gcctcaagca ggctttcgtc
     5521 gccgcgtgcg tctggatgtt ctcggcgagc tggtcgtacg acgtctacat cctcgcgcgc
     5581 gacgggcgct atcccgacac ctggctcgtg aacatcgccg cctcgtcggt gctctatatg
     5641 gccgcgggcc tgctctggaa cctcgactgg cgccccgggc gcggcgtgac gttctcgttc
     5701 ctcgagccgg gatggcccgg cgtgccgccg gccccgcagt tcgcgcgcct gatgggctac
     5761 gcgctgccgt tcatgctgat cgccgccgcg tcggtggcgt attttctcgc gaccgcctga
     5821 tggccgtgcc ggcgccggag tgcccgcgct gcaaagtggc gctgaccgac gccgaatccc
     5881 tcgcgtcgat ctgcggcgac gtcttcggcg acgagtacat cgacaccttc taccgctgcg
     5941 cgcactgcgc cacgtactgc cgcgagagct tccgcgaccg gttctccggg ccgagctcgg
     6001 cgcgcatgga gccgctcgag ccccgggcgg cggaggcgct gatcgagctc gtgcgccgct
     6061 gcccgtcgcc ggcgcacaag cactgccgtt gcgaagcgca ccgcgagtac ttcaggatct
     6121 agcggacgat ctcgtacttc tgagtcaggc tgttcaccag gaactgcgcg tcgaacacca
     6181 tctccggcac gtccggcttg ccgttgaagc aggcgatcag gttgaccgtg ccgggctggc
     6241 gcacgacggt ggtctggcct cccgaggtcg tcgtcgtggt cttcgtgcgg gtggtgtcgc
     6301 gcgacgtggt cgaggtctgg ctcgagaccg agcgcgtcgg ctccctgtgc gtcacgtatt
     6361 gcgactggtc cttctccgcg atgacgacga agtgcgtgaa cccgtgctcg agcgccacct
     6421 cggcgctgcg cagcagggcg aagtcctcgg cgcgctcgac gctcgtgtcg ccgttgccgg
     6481 tgaacgagac gcggaagatg ttctcgccga gctgcgtctc ggcgtagccg ccgaggaggc
     6541 cccggggctg gtacggcgtc gcgcacgcgg cgagcgcgag cacgaggagg gtcagtagca
     6601 cgctctgctt cgacatggcc gcggcaacat aggcgaggcg cgcttacgga tcgcttaccg
     6661 attggccgac acgcgcgcgc gcatggcctc ggtctccgcc gacggcggca cgccgagcac
     6721 caccgacagc atgtgccggc agcgccggta ggcctcgaga gcctcggccc tgcggccggc
     6781 ggcgagatac gccgccatca ggttgcggta cacgccctcg gcgagcgggt cgacctcgag
     6841 cgcgcggcgg tagagctcga cggcggcggg atgctcgccg cgctcctcgc gcgtcgcgcc
     6901 gcagcgctcg acgcagcgca ggaatttgct gcgcaggcgc tcgcgccgcg gcagcagcca
     6961 cggcgtctcc tcgtcgccct ggaggaagtg gccggcgtag aggccgagcg cgcgctcggc
     7021 ctcggcgagc tccccgccgt cgcgctcgca ggcgtcgagc gcctgctcga acgcgaacgc
     7081 gtcgacccag cagcaccggg ggttgagcgc gatcctgccg tcggcgacga ccaccgcgcc
     7141 ttccagcccg aggagcttgc gcagccggtg cagcgtgacg tcgagcgcgg tctgcgcggc
     7201 gtccccgtcg gacgccggcc acagcgccga ggcgatcgtg tcgctcgccg ctccgcgcgg
     7261 cccgaaggcg acgatcgcct tcagcaggtc gaggacgcgc tgctgcgcct tgccgcccga
     7321 tttcaccggg acgccgtcgc ggaccagctc gaagcgcccg agcg
//
LOCUS       DATDMW010000328         4191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2339259, whole genome shotgun sequence.
ACCESSION   DATDMW010000328 DATDMW010000000
VERSION     DATDMW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339259"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VI319_13730"
     CDS             complement(<1..189)
                     /locus_tag="VI319_13730"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6864952.1"
                     /translation="MLVAIDIGGTFTDLMAFDEATGRFSQAKSLTTPAQLVQGIIDCL
                     KKSAVDPGAIGELIHGSTT"
     gene            complement(196..1146)
                     /locus_tag="VI319_13735"
     CDS             complement(196..1146)
                     /locus_tag="VI319_13735"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY6864953.1"
                     /translation="MASSRYVPQGVIPAVLLPFHEDLSIDEASFRSHLRDVAAVEGLS
                     AITINAHSTEVASCTIDEQKRVLAIAKDEIGAKLPIVNGVYAEGSLEAARIARMAADG
                     GAAALLVFPPGPFTLGQRPEMVIEHFRRVADASGLPIIAFQYPLSGGQGYPLPTLVAL
                     AEAVPSVVAIKDWCSNPQLHERQVRTLQSLKRPVNVLTTHSSWLMTSLTTGCSGLLSG
                     SGSVSADLQSKLFRAVRANDLATARQLNDRIWPTAECFYAEPWVDMHNRMKEALVMLG
                     KLPRAVVRPPLVRVSDAERERVRAALVAAGLFERGQRKAA"
     gene            complement(1168..1590)
                     /locus_tag="VI319_13740"
     CDS             complement(1168..1590)
                     /locus_tag="VI319_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011204789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6864954.1"
                     /translation="MPVSHPFHLAFPVRDLAEARRFYGGLLGCPEGRSSDEWVDFDLY
                     GHQVVAHLAPGQCVEVSRNDVDGDAVPVRHFGVVLPMPEWERLAQKLKGAGTRFVIEP
                     HVRFKGQVGEQATMFFLDPSGNALEFKAFADLGQLFAK"
     gene            1647..2375
                     /locus_tag="VI319_13745"
     CDS             1647..2375
                     /locus_tag="VI319_13745"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY6864955.1"
                     /translation="MRYALYYWPSIPGRGELVRLALEDAGADYVDVARARNGVPTMMR
                     FMARRSLAHPPFACPFLVAGKLVIGQTANILLWLGPRLGLAPREEGARLWIHQLQLTM
                     ADLLVEAHDVHHPLGGWRYYEEQKRESKARAAGFRKERLPAFLDYFERVIRSNDRKYA
                     AKSGWLAGRRASYADLSLFQVVAGLRYAFPRAMARAERRRRRVAELHDRVARRASLAA
                     YLGSERRLPFNTMGIFRYYPELDG"
     gene            complement(2376..2555)
                     /locus_tag="VI319_13750"
     CDS             complement(2376..2555)
                     /locus_tag="VI319_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864956.1"
                     /translation="MSNFMRTHKLDWLVAAAIGAPLAILFAACAKDIADALGHIGDGS
                     LLALAAAANALTLVP"
     gene            2844..3752
                     /locus_tag="VI319_13755"
     CDS             2844..3752
                     /locus_tag="VI319_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEY6864957.1"
                     /translation="MKTPLIPALLAACALAACGGGGGGGGASAMRAPSSEQTPVTGSG
                     TDLTAMSTPTALSVGSVARMDTSAGAFTPAVTFSNAPTVRIAIPALGVDDTFTRADVV
                     STNVIAGVQIPTARRSDAQGVRTLTYIVPSNGPSGLRFSSLGIWDRADLTTGTINQQA
                     AISFGTRTAGNDIPTTGTATYSGFLLGNATELGGKQFSISALATATADFGARSVALSS
                     TGSGKTDRATGILVSDPGYNVTGTLTYAAGANALSGTVSSTNGRTGAAAGTFYGPQAA
                     ELGVTFSLANGASATPFVGGAAMKRQ"
     gene            complement(3754..>4191)
                     /locus_tag="VI319_13760"
     CDS             complement(3754..>4191)
                     /locus_tag="VI319_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864958.1"
                     /translation="EFYAQPQAEPAELGGVFTEETYGYSLPVLNQRRFPLRGYDPGDP
                     GLIGHRARLGTLEWRTPLADIDRATMLPPVGVNRVSLNVFADAGAAWDAGEQRRFRRG
                     YGVELLAEARAGYLYGAQFRAGIAHGVDENGRTVAYVRIGRSF"
BASE COUNT          612 a         1519 c         1476 g          584 t
ORIGIN      
        1 ggtggtcgag ccgtggatca gctcgccgat cgcgccggga tcgaccgcgc tcttcttcag
       61 gcagtcgatg atgccctgca cgagctgcgc cggcgtcgtg agcgacttcg cctgcgagaa
      121 gcggccggtc gcctcgtcga acgccatcag atcggtgaag gtgccaccga tgtcgatcgc
      181 gaccagcact tcggtctagg cggccttgcg ctggccgcgc tcgaacagcc cggcggcgac
      241 gagcgccgcg cgcacgcgct cgcgctcggc gtcggagacg cgtacgagcg gcggccgcac
      301 cacggcgcgc ggaagcttgc cgagcatgac gagcgcttcc ttcatgcggt tgtgcatatc
      361 gacccagggc tcggcgtaga agcactcggc ggtcggccag atgcggtcgt tcagctggcg
      421 cgcggtcgcg aggtcgttcg cgcgcacggc gcggaacagc ttcgactgaa gatccgcgct
      481 caccgagccg ctgccggaga gcagcccgct gcagcccgtc gtcagcgacg tcatcagcca
      541 ggagctgtgc gtcgtcagca cgttcacggg ccgcttcagc gactgcagcg tgcgcacctg
      601 gcgctcgtgc agttgcggat tggagcacca gtccttgatc gcgacgacgc tcggcacggc
      661 ttccgcgagc gcgaccagcg tcgggagcgg atagccctgt ccgccggaca aaggatactg
      721 gaacgcgatg atcggcagcc ccgacgcgtc ggcgacgcgc cggaagtgct cgatcaccat
      781 ctcgggacgc tgcccgagcg tgaacggccc cggcggaaac acgagcagcg ccgcggcgcc
      841 gccgtcggcg gccatgcgcg cgatgcgcgc cgcctcgagc gagccctcgg cgtagacgcc
      901 gttgacgatc ggcagcttcg cgccgatctc gtccttcgcg atcgcgagca cgcgcttctg
      961 ctcgtcgatc gtgcacgaag cgacctcggt cgaatgcgcg ttgatcgtga tcgccgacag
     1021 gccctcgacc gcggcgacgt cgcgcaggtg cgagcggaac gaggcttcgt cgatcgacag
     1081 gtcctcgtgg aacggcagca gcaccgccgg gatcacgccc tggggaacgt agcgggacga
     1141 cgccatgagg cctcctgttt cgcgagccta tttcgcaaaa agctgaccga ggtcggcgaa
     1201 cgccttgaac tcgagcgcgt tgccggacgg atcgaggaag aacatcgtcg cctgctcccc
     1261 gacctgcccc ttgaagcgca cgtgcggctc gatgacgaag cgcgtgccgg cgcccttcag
     1321 cttctgcgcg agccgctccc actcgggcat cggcagcacg acgccgaagt ggcgcaccgg
     1381 caccgcgtcg ccgtcgacgt cgttgcgcga cacctcgacg cactgcccgg gcgcgaggtg
     1441 cgcgaccacc tggtggccgt agagatcgaa gtcgacccac tcgtcgctcg agcgcccctc
     1501 ggggcagccg agcagcccgc cgtagaagcg gcgagcctcg gcgagatcgc gcacggggaa
     1561 ggcgaggtgg aagggatggc tgacgggcat gccgatactt taccggaaac ggtagcatcg
     1621 acgctttcgc agcccggagg ccccccatgc gctacgcgct ctactactgg ccctcgatcc
     1681 ccggccgcgg cgagctcgtc cgcctcgcgc tcgaggacgc gggcgccgac tacgtcgacg
     1741 tcgcgagggc gcgcaacggc gtccccacga tgatgcgctt catggcgcgg cgctcgctcg
     1801 cgcacccgcc cttcgcctgc ccgttcctcg tcgcggggaa gctcgtcatc ggccagacgg
     1861 cgaacatcct gctctggctc gggccgcgcc tcggcctcgc gccgcgagag gaaggcgcgc
     1921 gcctgtggat ccaccagctg cagctcacga tggccgacct gctggtcgag gcgcacgacg
     1981 tgcaccaccc gctcgggggc tggcgttact acgaggagca gaagcgcgag tcgaaggcgc
     2041 gcgcggcggg cttccgcaag gagcggctgc ccgcgttcct cgactacttc gagcgcgtga
     2101 tccgctcgaa tgatcgaaaa tacgccgcga agagcggctg gctcgccggg cggcgcgcga
     2161 gctacgcgga cctctcgctg ttccaggtgg tcgcgggcct gcgctacgcc ttcccgcgcg
     2221 cgatggcgcg cgccgagcgc cggcggcggc gcgtcgcgga gctccacgac cgggtcgcgc
     2281 ggcgcgcgag cctcgcggct tacctcggct cggagcggcg gctgccgttc aacacgatgg
     2341 ggatcttccg gtactacccc gagctcgacg gctgattacg gcaccagcgt gagcgcgttg
     2401 gccgcggccg cgagcgcaag caggctgccg tcgccgatgt gcccgagcgc gtcggcgatg
     2461 tccttcgcgc aggcggcgaa caggatcgcg agcggggcgc cgatggcagc ggcgacgagc
     2521 cagtcgagct tgtgggttcg catgaagttg ctcatggggt ttctcggcgc tcctttcaag
     2581 atggttcgtt gtgcgaagca gcaagcgccg tgccaaaggc gcccgttatc gcttcctcgt
     2641 tgatcgcaag cggtttttct cgggtgcgcc gcactgcgcc cgtcgtgccg gggcgacgca
     2701 tgccccgcga cggggccccg gggatcgccc gtgcaccagt ccgaagcggc ttgctccagc
     2761 cggctcaacg cggccaccga ccggacacgc gccctgcgcg cgtagcctcg ggctgttctc
     2821 cactcgtcgc gaaggatccc gccgtgaaga cgcctctcat ccccgcgctc ctcgcggcct
     2881 gcgcgctcgc cgcctgcggc ggcggcggag ggggcggcgg cgcgagcgcg atgcgcgcgc
     2941 cgagcagcga gcagacgccc gtcacgggct cgggcaccga ccttaccgcg atgtccacgc
     3001 cgaccgcgct cagcgtcggc tcggtcgcgc gcatggacac gagcgcgggc gcgttcacgc
     3061 ccgcggtcac gttctcgaac gcgccgacgg tgcgcatcgc catcccggcg ctcggcgtcg
     3121 acgatacctt cacgcgggcc gacgtcgtct cgaccaacgt catcgccggc gtgcagatcc
     3181 cgacggcgcg ccgctccgac gcacagggcg tgcgcacgct cacctacatc gtgccgtcca
     3241 acggtccctc gggtctgcgc ttctcgagcc tcgggatctg ggaccgcgcc gacctcacca
     3301 ccggaacgat caaccagcag gccgcgatct ccttcggcac gcgcaccgcg ggcaacgaca
     3361 tcccgacgac gggaacggcg acctacagcg gcttcctgct cggcaacgcg accgagctcg
     3421 gcggcaagca gttctcgatc agcgcgctcg cgaccgcgac cgcggacttc ggcgcgcgtt
     3481 cggtcgcgct ctcgagcacg ggatcgggca agaccgaccg cgcgaccggc atcctcgtct
     3541 ccgaccccgg ctacaacgtg acgggcacgc tcacgtacgc ggccggcgcg aacgcgctct
     3601 cgggcacggt gagctcgacc aacggcagga cgggcgcggc ggccggcacc ttctacggac
     3661 cgcaggccgc cgagctgggc gtgaccttct cgctcgcgaa cggcgccagc gcgaccccgt
     3721 tcgtcggcgg cgcggcgatg aagcgccagt gagctagaac gagcggccga tccggacgta
     3781 ggccacggtg cggccgttct cgtcgacgcc gtgcgcgatg ccggcgcgga actgcgcgcc
     3841 gtagagatag cccgcccgcg cctcggcgag cagctcgacg ccgtagccgc ggcggaagcg
     3901 gcgctgctcg cccgcgtccc aggccgcgcc cgcgtcggcg aacacgttca gcgacacgcg
     3961 gttcacgccg accggcggca gcatggtcgc gcggtcgatg tcggcgagcg gcgtgcgcca
     4021 ctcgagcgtg ccgaggcgcg cgcggtggcc gatgaggccc gggtcgcccg ggtcgtagcc
     4081 gcgcagcggg aagcgccgct ggttcagcac cggcagcgag tagccgtagg tctcctcggt
     4141 gaacacgccg cccagctcgg cgggctccgc ctgcggctgc gcgtagaact c
//
LOCUS       DATDMW010000329         3032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_346118, whole genome shotgun sequence.
ACCESSION   DATDMW010000329 DATDMW010000000
VERSION     DATDMW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346118"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1666)
                     /locus_tag="VI319_13765"
     CDS             complement(104..1666)
                     /locus_tag="VI319_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6864959.1"
                     /translation="MERSTEPTTDPVKFLLVGDALFQRAYGRLLRDRGLEVVEVAEEE
                     AIEAAQTLDPAGVVLRLRRGVPAALSIFESIRRACPALPILVVSQQPTVAEAVKLMQL
                     GAHACVPLLIEPTGAASPSPELLENLQAILHSRQAASSSDRTGRMGPVAAGHGLVGGS
                     LPMRAVLRDVALVAPTRTTVLILGETGTGKERIAQAIHQGSPRRGASFVAVNCAAISE
                     TLLESALFGHEKGAFSGAHCRREGLFKIADGGTLFLDEIGETSPALQAKLLRVLQERQ
                     FERVGSTAPVSTDVRVIAATNRDLRQMVAERTFRSDLFYRLNVMVINVPALRHRREDI
                     GPLVTHILARLSFELGIPRPAVGPEVLALLEEYPWPGNVRELENVLERLLVVSQDRTV
                     TLSDLPAELRETTRPPSFVAGDPSSMVADGRPLIPGASFREIERHAILATYEACGQSP
                     SRTAHVLGLSPRTVHYRLREYRGETGRRTQRLGGGWRAPEETWAERARGPLPARVPPT
                     GELPGLASERRY"
     gene            2088..>3032
                     /locus_tag="VI319_13770"
     CDS             2088..>3032
                     /locus_tag="VI319_13770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6864960.1"
                     /translation="MRVAFFGSSLVSAYWNGAASYYRGLLKALAARGFSITFYEPDVY
                     QRQAHRDMEDPTWARVMVYPPTADAARAAMRQAESADLVVKASGVGIFDALLEREILA
                     LKSRERLVAFWDVDAPATLDRLFGDAGDPFRELVPQYDAIFTYGGGPGVVRAYQALGA
                     GVCLPVYDALDPEMHHPAPRDLRHACALTFLGNQMPDREARVEEFFFSVARRRPYWKF
                     VLGGSGWDPRQVPRNVVTMGHVHTYDHNTLSSSALAVLNLHRESRYGYSPATRLFEAA
                     GSASCIITDESPGLETFLTPGEELLVARDGQDVAEYLDD"
BASE COUNT          448 a         1076 c         1067 g          441 t
ORIGIN      
        1 gaccgcgtcg cctcccgatc gaaacctgtg gcgtgtgagg gacggcgaca aggaccggcc
       61 cgacggcggc gtcgggtggc cctctagggg gcctagagcc tcttcagtag cgccgctcgc
      121 tcgcgagccc gggcagctcg cccgtcggcg gcacgcgggc cggcagcggc ccgcgcgcgc
      181 gctcggccca cgtctcctcg ggcgcccgcc agccgccgcc gaggcgctgc gtgcgccgcc
      241 cggtctcgcc gcggtactcg cgcagccgat agtggaccgt gcgcggcgaa aggccgagca
      301 cgtgcgccgt gcggctgggg ctctggccgc aggcttcgta ggtcgccaag atggcgtgcc
      361 gttcgatctc gcggaagctc gcgcccggga tcagcggtcg cccgtcggcg accatcgagc
      421 tcgggtcacc cgcgacgaag gaaggcggcc gcgtcgtctc gcgcagctcc gccggcagat
      481 cgctgagcgt cacggtgcga tcctgcgaga ccaccagcag ccgctcgagc acgttctcga
      541 gctcgcggac gttgcctggc cacgggtact cctccagcag cgccagcacc tcgggcccca
      601 cggcggggcg cgggatgccg agctcgaacg agagccgcgc gaggatgtgc gtcaccagcg
      661 gcccgatgtc ctcgcgccgg tgccgcagcg ccgggacgtt gatgaccatc acgttgagcc
      721 gatagaacag gtccgagcgg aaggtgcgct ccgccaccat ctgccgcaga tcgcggttgg
      781 tggcggcgat cacccgcacg tccgtcgaga cgggcgccgt gctgcccacg cgctcgaact
      841 gtcgctcctg caggacgcgc agcagcttgg cctgcagcgc cggcgaggtc tcgccgatct
      901 cgtcgaggaa cagcgtgccg ccgtcggcga tcttgaacag gccctcgcgg cggcagtgcg
      961 cgccgctgaa cgcgcccttc tcgtgaccga agagcgcgct ctcgagcagc gtctcggaga
     1021 tcgccgcgca gttcacggcg acgaaagacg ccccgcgccg cggcgagcct tgatggatcg
     1081 cctgggcgat gcgctccttg ccggtgcccg tctcgccgag gatcagcacc gtcgtccgcg
     1141 tgggcgcgac cagcgcgacg tcgcgcagga cggcccgcat gggcaggctc ccgcccacca
     1201 ggccgtgccc ggcggcgacc ggccccattc gtcccgtgcg atcggaggac gaggcggcct
     1261 gccggctgtg caggatcgct tgcaagttct cgagcagctc gggcgacggc gacgccgccc
     1321 ccgtcggctc gatgaggaga ggcacgcacg cgtgcgcgcc gagctgcatg agcttcaccg
     1381 cctcggccac cgtgggctgc tggctgacga ccaagatggg caacgcgggg cacgcgcgcc
     1441 gaatcgactc gaagatggac agcgcggccg gcacaccgcg gcgcaggcgc agcaccacgc
     1501 cagcgggatc cagcgtctgc gcggcttcga tcgcctcctc ttcggcgacc tcgaccacct
     1561 ccaggccgcg gtcacgcagc aggcggccat aggcgcgctg aaacaacgcg tcgcccacca
     1621 gcaggaattt gacgggatcg gtcgttggct cggtcgaacg ttccacggcg agacctcggg
     1681 gttggcgtcc ggattccgat atagccacga acccagaagc cgaaaggtct ctgcgcggcg
     1741 aaagttcaat tatttctgag aggtgcgtgc ggctcccacc cggacgagtc ccgacgtttg
     1801 ccgcgcagcg tgaaggccgc gcgcaggaac ccaaaatgtc gccatgggcg ggcgatcgat
     1861 gcttgcggcg atgcttattc ttgccgcgca tacggggctc gacgcgccct ccccgactcg
     1921 tttgggcgcc ccatttgagg agcgaggatg caggtcgtgg tgttctgtca ctcggtggtg
     1981 ctcgatcggc agcgggaggc ggcctgatgt cgacgcgcac ggacggccca gcgcctggcg
     2041 cgcgcgcgcg tcgcacgaag gttcgcggcc ccaagaacgt gcgctcgatg cgcgtcgcgt
     2101 tctttggctc gagcctggtg tcggcctact ggaacggcgc cgcgagctac taccgaggcc
     2161 tgctcaaggc gctggccgcg agaggcttca gcatcacctt ctacgagccc gacgtgtatc
     2221 agcgccaggc tcaccgggac atggaggacc cgacctgggc gcgggtcatg gtctatccgc
     2281 ccacggcgga cgccgcgcgc gcggccatgc gccaagcgga gagcgccgac ctcgtcgtca
     2341 aagccagcgg cgtcgggatc ttcgacgcgc tcctcgaacg agagatcctc gcgctcaaga
     2401 gccgtgagcg cctggtggcg ttctgggacg tcgacgcgcc cgcgacgctg gaccgtctgt
     2461 tcggcgacgc cggcgatccg ttccgcgagc tcgtcccgca gtacgacgcg atcttcacct
     2521 acggcggcgg gcccggcgtc gtcagggcct accaggcgct cggagccggg gtctgcctgc
     2581 cggtctacga tgccctcgat cccgagatgc atcaccccgc cccgcgcgat ctgcggcacg
     2641 cgtgcgccct gaccttcttg ggcaaccaga tgcccgatcg cgaagcgcgc gtcgaggagt
     2701 tcttcttctc ggtggcgcgg cgccgcccct actggaagtt cgtgctgggc ggcagcggat
     2761 gggatccgcg gcaagtgccg cggaacgtcg tcacgatggg gcacgtccac acgtacgacc
     2821 acaacaccct gagctcgtcg gcgctggccg tgctgaatct gcaccgggag agccgctacg
     2881 ggtactcgcc cgcgacgcgc ctcttcgaag ccgcggggtc ggcgagctgc atcatcaccg
     2941 acgagagccc gggcctcgag acgttcctga ctccgggcga agagctgctc gttgcgcgtg
     3001 acgggcagga cgttgccgaa tacctggacg at
//
LOCUS       DATDMW010000330         8202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_471194, whole genome shotgun sequence.
ACCESSION   DATDMW010000330 DATDMW010000000
VERSION     DATDMW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471194"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VI319_13775"
     CDS             <1..271
                     /locus_tag="VI319_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEY6864961.1"
                     /translation="VLVVPHHGSTSSSTPDFVHAVAPRRALFAVGWRNRFGHPREAVL
                     ERYRGVGSEILRSDESGAITLRDFAGGFRVKEWRKAEQRYWASIR"
     gene            321..959
                     /locus_tag="VI319_13780"
     CDS             321..959
                     /locus_tag="VI319_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864962.1"
                     /translation="MWAVGIIWAVFAPLLLAPVVALLAWLLSKARIRLAVPLAVLAVV
                     LPVSIFWFIDWSEFDKICKTEGAPVVYRRSLAEGIFLNSGTSNSFGMRYLQEEGFSWV
                     EARSVTEPGAWVRYERSANGTISTTRISALTARYEVREDFIKPDRHTGLSRTQVIDRV
                     TGELVAKAASANFDGGRMNAVLGVWGSRSCPSAMSSPEGFDGYYHLASHALR"
     gene            997..1860
                     /locus_tag="VI319_13785"
     CDS             997..1860
                     /locus_tag="VI319_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864963.1"
                     /translation="MPARLHAVIVIEGDPDALAACRARVNALLAGDFDEELRERHTAG
                     RLEYELRVSGGIPFPPFVAASQEFPDLELKVEWSEPSQGRSGRAIIANGALREQASQA
                     GAATGTLLLHAQADAEGRLALALACRAWRGAWHGYAIAAEQHAFFRVDGAKDAFALRA
                     SGGVEGRWAERWSAAEEGVRYEEIDEAVADDELRELDAIAREFAAEWIWFAEAPAEET
                     AVERSRYATYGVPVSGANLRSEKLRRVLQAEAGGGSSFSTFGADTRWILQLMHDFWLA
                     AGETEMEPPAL"
     gene            1857..2714
                     /locus_tag="VI319_13790"
     CDS             1857..2714
                     /locus_tag="VI319_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6864964.1"
                     /translation="MTAAFRRRSVQCASPSGLHRVSYVEWGERGNPRVLVCAHGLTRC
                     ARDFDFLAAEMSRTHRVICPDVPGRGESQWLRNPMEYVVPTYVADMVTLIARADVESV
                     QWVGTSLGGLIGMGLAAFPEGLVSRLVLNDSGPLVTAQSLDRIGSYVGKAPSFPDLET
                     AEYYVRTVSAPFGPHSDAEWRFLTEHVVRRQPDGSLRMHYDPALAVPFNAEQPHKDIE
                     LWKLYDAIRCPTLVLRGESSDLLTRETAGAMTERGPRAKLVEIAGVGHAPTLIHADQI
                     AIVRDFLLG"
     gene            2719..3702
                     /locus_tag="VI319_13795"
     CDS             2719..3702
                     /locus_tag="VI319_13795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY6864965.1"
                     /translation="MRVIAALAAALASSAAPAQGAPAFVVPLQCAIGKTCIVQNYVDR
                     DPGPAARDYRCGFLTYQGHKGTDIRVIDAEAWRRGVPVLAADDGRVLAVRDGMPDVSV
                     RALRPGVVAGREAGNSVIIDHGDGWETQYAHLRRHSIAVRAGDVVSRGQPIGEVGMSG
                     ETEFPHLHFEVRHDRRTVDPFVGAEGGEPCRAGAKPLWSPDALAALAYTATGVLGAGI
                     SGAPPAIGDWGMVGDGAVPFGRASRAAVFWVQIFGARAGDLETLRLIGPDGRVLAARS
                     ARIPGERAQSLAYAGVRRGSDWASGLYRGEYTLVRGGEKRMALVREVRLGP"
     gene            complement(3686..4492)
                     /locus_tag="VI319_13800"
     CDS             complement(3686..4492)
                     /locus_tag="VI319_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY6864966.1"
                     /translation="MGFLREWRRRRTLKSARLDEDLWRRVYGDLSFLRGLSEDEAARL
                     RDLVVLFLAEKEMHGAHGFELTDEIRLAIALQACLPILNLGLSAYDGWVGIVVYPGQF
                     RVVKEEVDEDGVVHVYEDEQAGEAWPGGPVILSWEDVGLSGAGYNVVIHEFAHKIHLD
                     GPGDDDFPDPREGMDAEKWRETLDAAYDRFCADVDSGRPVLVDDYAAEHPSEFFAVMS
                     EAFFTDSAVLARDYPALYEQLARFYRQDPAERLPAKPAEKRPPKRAKDRA"
     gene            4532..6700
                     /locus_tag="VI319_13805"
     CDS             4532..6700
                     /locus_tag="VI319_13805"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY6864967.1"
                     /translation="MVTVVRPAAADEAQALASGLAPEEREPLERALAFARETYGDAVL
                     GTGEPALAHALGLAANVAALRLDAEARIAGLMFAAPEYVEDSDEKIRRLFGDKVAALV
                     AGITRLNELRVVVRSAGTQTRDARAQAETLRKMLLAMVQDIRVVLLRLASRAQTLRFL
                     AAAPAAERREIARETLDIYAPLANRLGVWQLKWELEDLSFRYLEPDLYRKIAGLLEER
                     RTEREAFIAAARERLSGALAAAGIAAEVVGRPKHIYSIWSKMRAKALDFSQVHDVRAL
                     RVLVGEVKDCYAALGVVHDLWTPIPQQFDDYISRPKENAYRSLHTAVMAEDGRALEVQ
                     IRTHEMHREAELGVAAHWRYKEGARRPARGADALDERIAWLRQMLAWRDEIVDASDAP
                     DLKALDDTVYALTPQGRVIDLPKGSTPVDFAYALHTDVGHRCRGAKVNGAMVPLDTPL
                     ANGDRVEIVTAKQGGPSRDWLNPDLGYIASSRARNKVRQWFNREEMEQTIAAGRAAVE
                     RELQREGRKDASLEQLAAGLGFDDTEDFFAAVGREEVGARQIQAALRGEDAAAPPQQP
                     AVRPSRAPAGGDGILIVGVDRLLTQLARCCKPVPPDPIAGFVTKGRGVSIHRRDCPSL
                     ARLVARAPERRIAADWGEQGARRFAADLALQATDRQGLLRDVTEVLSRERINVTAVST
                     QTRHSIASMQFTVEVDDSAHLQRALALLREVRGVMTAARR"
     gene            6726..7469
                     /gene="surE"
                     /locus_tag="VI319_13810"
     CDS             6726..7469
                     /gene="surE"
                     /locus_tag="VI319_13810"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY6864968.1"
                     /translation="MRILLSNDDGYFAPGIAILADALADLGSITVVAPERDRSGASNS
                     LTLDRPLSVRKSANGYLFVNGTPTDCVHLAVTGLLDELPDVVISGINLGANMGDDTIY
                     SGTVAAAMEGYLLGIPSFAVSLTSKAGKHYDTAARIARELVERLQRRPAPSPVLLNVN
                     VPDIAFDELQGLEVTRLGKRHKAEPVVKAKSPRNETIYWVGAAGAAQDAGPGTDFHAV
                     SAHRVSLTPLQIDLTHSQQIPGVRAWLGQ"
     gene            7508..8137
                     /locus_tag="VI319_13815"
     CDS             7508..8137
                     /locus_tag="VI319_13815"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY6864969.1"
                     /translation="MTSQRTRDRMIARLREKGIRDETVLAAMASVPRHLFVDEALASR
                     AYEDSALPIGFEQTISQPFVVARMLAELRDGRPLGRVLEVGTGCGYQAAVLAQVATEV
                     YSIERIAGLLDRARANLRTLRLANLRLVHGDGARGLPDAAPFDGIVVAAASPKASELL
                     LQQLAVGGRMIAPLGAEEQALCLIERTTKGFTEKWLDAVRFVPLRAGKQ"
BASE COUNT         1140 a         2949 c         2989 g         1124 t
ORIGIN      
        1 cgtgctcgtc gttccgcatc acggcagcac gagctcctcg acgccggatt tcgtgcacgc
       61 ggtggcgccg cgccgggcgc tgttcgcggt cggctggcgc aaccgcttcg ggcatccgcg
      121 cgaggccgtg ctcgagcgct atcgcggggt cggcagcgag atcctgcgca gcgacgagtc
      181 gggcgcgatc accctgaggg attttgccgg cgggttccgc gtgaaagaat ggcgcaaggc
      241 ggagcagcgg tactgggcat cgattcgttg agcccgggcc gcggtatcat ggagcagaca
      301 ctttcgaggg agcgcacccg atgtgggccg tcggaatcat ttgggcggtg ttcgcgccgc
      361 tcttgctcgc gccggtggtt gcgctgcttg catggctcct gagcaaagcg cggatcaggc
      421 tcgcggtccc gctggcggtg cttgccgtgg tgctcccggt gtcgatcttc tggttcatcg
      481 actggtccga gttcgacaag atctgcaaga ccgagggcgc cccggtcgtc tatcgccgca
      541 gcctagcgga gggcatcttc ctgaactccg ggacctcgaa cagcttcggc atgcgctatc
      601 tccaggagga agggttcagc tgggtggagg cgcgcagcgt gaccgagccc ggcgcatggg
      661 tccggtacga gcgctcggca aacgggacca tcagcaccac gcgcatatcg gcgctcaccg
      721 cccgctacga ggtccgcgag gatttcatca agcctgatcg ccataccggc ctgtccagga
      781 cgcaagtcat cgatcgcgtt accggcgagc ttgtcgccaa ggccgccagc gcgaacttcg
      841 acggcggaag gatgaacgcc gtcctgggcg tgtggggatc gcgatcgtgc ccgagcgcga
      901 tgagctcgcc ggagggcttc gacggctact accacttggc gagccacgcg ctgcgttagc
      961 tatactggct gctccaccgg aaggaccgca gcaaccttgc ccgcccgcct ccacgccgtc
     1021 atcgtcatcg aaggcgaccc cgacgcgctc gccgcgtgcc gcgcgcgcgt gaacgcgctg
     1081 ctcgccggcg atttcgacga ggagctgcgc gagcggcaca cggcggggcg gctcgagtac
     1141 gagctgcgcg tctcgggcgg gatcccgttt ccgccgttcg tcgcggcgtc gcaggaattc
     1201 ccggatctcg agctgaaggt ggagtggtcg gagccttccc agggccggag cggccgcgcg
     1261 atcatcgcga acggcgcgct gcgcgagcag gcgtcgcagg cgggcgcggc gaccgggacg
     1321 ctgctcctgc acgcgcaggc cgacgccgaa gggcgcctcg cgctcgcgct cgcgtgccgc
     1381 gcgtggcgag gcgcgtggca cggctacgcg atcgcggccg agcagcacgc gttcttccgc
     1441 gtcgacggcg cgaaggacgc cttcgcgctg cgcgcctcgg gcggcgtcga gggccgctgg
     1501 gccgagcgct ggagcgcggc cgaggagggc gtgcgctacg aggagatcga cgaggcggtc
     1561 gccgacgacg agctgcgcga gctcgacgcg atcgcgcgcg agttcgccgc cgagtggata
     1621 tggttcgccg aggccccggc cgaggagacc gcggtcgagc gcagccgcta cgcgacctac
     1681 ggcgtgccgg tctcgggcgc gaacctgcgc tcggaaaagc tgcgcagggt gctgcaggcg
     1741 gaggcgggcg gcggctcgtc gttcagcacg ttcggcgccg acacgcgctg gatcctccag
     1801 ctcatgcacg acttctggct cgccgcgggc gagaccgaga tggagccgcc ggcgctatga
     1861 cggccgcgtt ccggcgccgg agcgtgcagt gcgcgagccc gagcggcctg caccgcgtgt
     1921 cgtacgtcga gtggggcgag cgcggcaacc cgcgcgtgct cgtctgcgcg cacggcctga
     1981 cgcgctgcgc gcgcgacttc gatttcctcg cggcggagat gtcgaggacg caccgcgtga
     2041 tctgccccga cgtccccggg cgcggcgagt cgcagtggct gcgcaatccc atggagtacg
     2101 tcgtgccgac ctacgtcgcc gacatggtga cgctgatcgc gcgcgcggac gtcgagtcgg
     2161 tgcagtgggt gggcacctcg ctcggcgggc tgatcggcat gggcctcgcc gcgtttcccg
     2221 aaggcctcgt ctcgcggctg gtgctgaacg actcgggccc gctcgtcacc gcgcaatcgc
     2281 tcgaccgcat cggctcctac gtcggcaagg cgccgagctt ccccgacctc gagaccgccg
     2341 agtactacgt gcgcaccgtc agcgcgccct tcgggccgca cagcgacgcc gagtggcgct
     2401 tcctcaccga gcacgtcgtg cgccggcagc ccgacggcag cctgcgcatg cactacgatc
     2461 ccgcgctcgc ggtgccgttc aacgccgagc aaccgcacaa ggacatcgag ctgtggaagc
     2521 tctacgacgc gatccgctgc ccgacgctgg tgctgcgcgg cgagtcctcc gacctgctca
     2581 cgcgcgagac cgcgggcgcg atgaccgagc gcggcccgcg cgcgaagctc gtcgagatcg
     2641 cgggcgtggg ccacgcgccg acgctcatcc acgcggacca gatcgcgatc gtccgcgact
     2701 tccttctcgg ctgacgcgat gagggtcatc gcggcgctcg cggcggcgct cgcttcgtcc
     2761 gccgcgcccg cgcagggcgc gcccgccttc gtcgtgccgc tgcaatgcgc gatcggcaaa
     2821 acgtgcatcg tgcagaacta cgtcgaccgc gaccccggcc ccgcggcgcg cgactaccgc
     2881 tgcggcttcc tcacctacca aggccacaag ggcaccgaca tccgcgtgat cgacgccgag
     2941 gcgtggcgcc gcggcgtgcc cgtgctcgcc gcggacgacg ggcgcgtgct cgccgtgcgc
     3001 gacggcatgc ccgacgtcag cgtgcgcgcg ctgcgccccg gcgtggtcgc ggggcgcgag
     3061 gcgggcaact ccgtgatcat cgatcacggc gacggctggg agacgcagta cgcgcacctg
     3121 cgccggcaca gcatcgcagt gcgcgcgggc gacgtcgtgt cccgcggcca gccgatcggc
     3181 gaggtcggga tgtcgggcga gaccgagttc ccgcacctgc acttcgaggt gcggcacgac
     3241 cggcggacgg tcgacccgtt cgtcggcgcc gagggcggcg agccgtgccg cgccggcgcg
     3301 aagcccctgt ggtcgcccga cgcgctcgcc gcgctcgcgt atacggcgac cggcgtgctc
     3361 ggcgccggga tctcgggcgc gccgccggcg atcggcgatt ggggcatggt cggcgacggc
     3421 gccgtgccgt tcggccgcgc gtcgcgcgcg gcggtcttct gggtgcagat cttcggcgcg
     3481 cgcgcgggcg acctcgagac gcttcgcctg atcggcccgg acgggcgcgt gctcgcggcg
     3541 cgcagcgcgc gcattccggg cgagcgcgcg caatcgctcg cctacgcggg cgtgcgccgc
     3601 gggtcggact gggcgagcgg actctatcgc ggcgagtaca cgctggtgcg cggcggcgag
     3661 aagcggatgg cgctcgtgcg cgaggtcagg ctcggtcctt agcgcgcttc ggcggccgct
     3721 tctccgccgg cttcgccgga agccgctcgg cgggatcctg gcggtagaag cgcgcgagct
     3781 gctcgtagag cgcgggatag tcgcgcgcga gcaccgccga gtcggtgaag aacgcctcgc
     3841 tcatcaccgc gaagaactcc gacgggtgct ccgctgcgta atcgtcgacc agcaccggcc
     3901 gccccgagtc gacgtccgcg cagaagcggt cgtaggccgc gtcgagcgtc tcgcgccact
     3961 tctccgcgtc catgccctcg cgcggatcgg ggaaatcgtc gtcgccgggg ccgtcgaggt
     4021 ggatcttgtg cgcgaactcg tggatcacca cgttgtagcc cgcgcccgag agcccgacgt
     4081 cctcccacga caggatcacc gggccgccgg gccaggcctc gcccgcctgc tcgtcctcgt
     4141 agacgtgcac gacgccgtcc tcgtcgacct cttccttcac cacgcggaac tgcccggggt
     4201 agacgacgat cccgacccag ccgtcgtagg cggagagccc gaggttcagg atcggcaggc
     4261 aggcctggag cgcgatcgcg agccggatct cgtcggtgag ctcgaagccg tgcgcgccgt
     4321 gcatctcctt ctcggcgagg aacagaacga cgagatcgcg caggcgagcg gcttcgtcct
     4381 cggacagccc gcgcaggaac gagagatcgc cgtagacgcg ccgccacagg tcctcgtcga
     4441 gccgcgcgct cttcagcgtg cggcggcggc gccattcgcg caggaagccc atctctcggg
     4501 aacgcctcgg aaaaagggta cattatccac catggtcacc gtcgtccgtc ccgcagctgc
     4561 cgacgaggcg caggcgctcg cctcgggcct cgcgcccgag gagcgcgagc cgctcgagcg
     4621 cgcgctcgcg ttcgcgcgcg agacctacgg cgacgcggtc ctcggcacgg gcgagcccgc
     4681 gctcgcgcac gcgctcgggc tcgcggccaa cgtcgcggcg ctcaggctcg acgccgaggc
     4741 gcgcatcgcc gggctgatgt tcgcggcgcc cgagtacgtc gaggacagcg acgagaagat
     4801 caggcgcctc ttcggcgaca aggtcgccgc gctggtcgcg ggcatcacgc ggctgaacga
     4861 gctgcgcgtc gtcgtgcgct cggcgggcac gcagacgcgc gacgcgcgcg cccaggccga
     4921 gacgctgcgc aagatgctgc tcgcgatggt gcaggacatc cgcgtggtgc tgctgcggct
     4981 cgcgagccgc gcgcagacgc tgcgcttcct cgcggctgcg cccgccgccg agcgccgcga
     5041 gatcgcgcgc gagacgctcg acatctacgc gccgctcgcg aaccggctcg gcgtgtggca
     5101 gctgaagtgg gagctcgagg acctgtcgtt ccgctacctc gagcccgacc tgtacaggaa
     5161 gatcgccggc ctgctcgagg agcgccgcac cgagcgcgag gccttcatcg ccgccgcgcg
     5221 cgagcggctc tcgggcgcgc tcgctgcggc cggcatcgcg gccgaggtcg tcgggcggcc
     5281 gaagcacatc tacagcatct ggagcaagat gcgcgcgaag gcgctcgact tctcgcaggt
     5341 gcacgacgtg cgcgcgctgc gcgtgctcgt cggcgaggtg aaggattgct acgccgcgct
     5401 cggcgtggtg cacgacctgt ggacgccgat cccgcagcag ttcgacgact acatctcgcg
     5461 ccccaaggag aacgcgtacc ggtcgctcca caccgcggtc atggccgagg acggccgcgc
     5521 gctcgaggtg cagatccgca cgcacgagat gcaccgcgag gccgagctcg gcgtcgccgc
     5581 gcactggcgc tacaaggagg gcgcgcgccg cccggcgcgc ggcgccgacg cgctcgacga
     5641 gcgcatcgcc tggctgcgcc agatgctcgc ctggcgcgac gagatcgtcg acgcctccga
     5701 cgcgcccgac ctgaaagcgc tcgacgacac ggtctacgcg ctcaccccgc agggccgcgt
     5761 gatcgacctg ccgaagggct cgacgccggt cgacttcgcg tacgcgctgc acaccgacgt
     5821 cggccaccgc tgccggggcg cgaaggtgaa cggcgccatg gtgccgctcg acacgccgct
     5881 cgcgaacggc gaccgcgtcg agatcgtcac cgcgaagcag ggcggcccca gccgcgactg
     5941 gctgaacccg gacctcgggt atatcgccag ctcgcgcgcg cgcaacaagg tgcgccagtg
     6001 gttcaaccgc gaggagatgg agcagaccat cgcggcggga cgcgccgcgg tcgagcgcga
     6061 gctgcagcgc gagggccgca aggacgcgag cctcgagcag ctcgcggccg gtctcgggtt
     6121 cgacgacacg gaggatttct tcgcggcggt cgggcgcgag gaggtcggcg cgcgccagat
     6181 ccaggcggcg ctgcgcggcg aggacgccgc ggcgccgccc cagcagcccg cggtgcggcc
     6241 gagccgcgcg cccgcgggcg gcgacggcat cctgatcgtc ggcgtcgacc gcctgctcac
     6301 gcagctcgcg cgctgctgca agccggtgcc gcccgacccg atcgcgggct tcgtcaccaa
     6361 gggccgcggc gtgtcgatcc accggcgcga ctgcccgagc ctcgcgcggc tggtcgcgcg
     6421 cgcgcccgag cgccgcatcg ccgccgactg gggcgagcag ggcgcccgcc gcttcgcggc
     6481 cgacctcgcg ctgcaggcga ccgaccgcca gggcctgctg cgcgacgtca ccgaggtgct
     6541 gtcgcgcgag cgcatcaacg tcaccgcggt gagcacgcag acgcggcact cgatcgcctc
     6601 gatgcagttc acggtcgaag tcgacgacag cgcccacctg cagcgcgcgc tcgcgctgct
     6661 gcgcgaggtg cgcggggtga tgacggcggc gcggcgctga ggccgcctcc gctataattc
     6721 ggcccgtgcg gatcctcctg agcaacgacg acgggtactt cgcgcccggc atcgcgatac
     6781 tcgccgacgc gctcgcggat ctcggctcca tcaccgtcgt cgcgcccgag cgcgaccgca
     6841 gcggcgcctc caactcgctc acgctcgacc ggccgctgtc ggtgcgcaag tcggcgaacg
     6901 gctacctgtt cgtgaacggc acgccgaccg attgcgtcca cctcgccgtc accgggctgc
     6961 tcgatgagct gcccgacgtc gtcatctcgg gcatcaacct cggcgccaac atgggcgacg
     7021 acaccatcta ctcgggcacg gtcgcggcgg cgatggaagg ctacctgctc ggcatcccgt
     7081 ccttcgcggt ttcgctcacg agcaaggccg gcaagcacta cgacactgcg gcgcgcatcg
     7141 cgcgcgagct ggtcgagcgc ctgcagcggc ggccggcgcc ttcgccggtg ctgctgaacg
     7201 tcaacgtgcc cgacatcgcg ttcgacgagc tgcagggcct cgaggtgacg cggctcggca
     7261 agcgccacaa ggccgagccg gtggtcaagg cgaagagccc gcgcaacgag acgatctact
     7321 gggtcggcgc cgcgggcgcg gcgcaggacg caggtcccgg caccgacttc cacgccgtgt
     7381 cggcccatcg cgtctcgctc acgccgctgc agatcgacct cacgcattcg cagcagatcc
     7441 cgggcgtgcg ggcctggctc ggccagtgag cgggcttccg ccgggggcgc gcgagcgcgg
     7501 catcggcatg acgtcgcagc gcacgcgcga ccgcatgatc gcgcggctgc gcgagaaggg
     7561 gatccgcgac gagacggtgc tcgcggcgat ggcctcggtg ccgcgccacc tgttcgtcga
     7621 cgaggcgctc gcgagccgcg cctacgagga cagcgcgctg ccgatcggct tcgagcagac
     7681 gatctcgcag cccttcgtcg tcgcgcgcat gctcgccgag ctgcgcgacg gccggccgct
     7741 cggccgggtg ctcgaggtcg gcaccggctg cggctaccag gcggcggtgc tggcgcaggt
     7801 cgccacggag gtctactcga tcgagcgcat cgcggggctc ctcgatcgcg cgcgcgcgaa
     7861 cctgcgcacg ctgcgcctcg cgaacctgag gctcgtgcac ggcgacggcg cgagaggcct
     7921 gcccgatgcg gcgcccttcg acggcatcgt cgtcgcggcg gcctcgccca aagcgtcaga
     7981 attgcttttg cagcagcttg ccgtgggtgg cagaatgatc gcgccgctcg gagcggagga
     8041 gcaggcgctg tgcctgatcg agcggacgac gaagggattc accgaaaaat ggctggacgc
     8101 ggttcgattc gtgcccttgc gcgccgggaa gcagtgatcc ggctcgccgc gcgctcgctg
     8161 cccgcgctgt gcgcgctggc gctcgcggcc gggtgcacga cc
//
LOCUS       DATDMW010000331         9765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2340123, whole genome shotgun sequence.
ACCESSION   DATDMW010000331 DATDMW010000000
VERSION     DATDMW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340123"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VI319_13820"
     CDS             complement(<1..397)
                     /locus_tag="VI319_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEY6864970.1"
                     /translation="MRRVPWPEAKNVLAVRLDNIGDVLMTTPAIRALKESAPGRRVTL
                     LTSPAGAQLAPYVREIDHVITFAAPWMKATGDGPTMDGAMIVQLASKSFDAAVIFTVY
                     SQNPLPAAYLAYLAGIPLRLAHCRENPYRL"
     gene            complement(394..1005)
                     /locus_tag="VI319_13825"
     CDS             complement(394..1005)
                     /locus_tag="VI319_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEY6864971.1"
                     /translation="MAMTRESDRAVFLDKDGTLIDDVAYNVDPVRITLARGAAEGLYL
                     LVRRGYRIVVVSNQPGVALGIFPEHALGGVESRLRELLRDVGVPLSGFHYCPHLPGGR
                     LPEYAVPCACRKPASGLLSRAAREHGLDLASSWMVGDILDDVEAGRGAGCRTVLIDNG
                     NETEWDLTPGRRPDKVAADLFEAAALIVSDDARVGPVLMCGTA"
     gene            complement(996..1712)
                     /locus_tag="VI319_13830"
     CDS             complement(996..1712)
                     /locus_tag="VI319_13830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6864972.1"
                     /translation="MSVGRLEGKVVFVTGGGRGLGAAICRACAQDGAQVAVADLDLAR
                     AQAIAAEVGGCAIRVDVGDEDPCERALAETLERLGRLDALVNNAGVDYTLSLEELSVE
                     QWDRVIRTNLRGPFLLAKRAAAIMARQGGGHIVNIASTAAKRAWANASAYHASKWGLL
                     GFSHALHAELRPLGIKVSAVVAGGMRTPFLLDRFPDLDPGVLQDPANVAATVAFLLAM
                     PEESVIPEVMVIPMRESSWP"
     gene            complement(1709..2980)
                     /locus_tag="VI319_13835"
     CDS             complement(1709..2980)
                     /locus_tag="VI319_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY6864973.1"
                     /translation="MLRKIALVSDHASPLAAAGGVDSGGQNIYVAQLARHLAALGYAV
                     DVFTRRDAPDQPLVQEWARGVRVIHVPAGPARYVRKEDLLPLMEEFSACVCSFCRREG
                     GYDLVHANFFMSGLVAIKLKQRFDIPFVVTFHALGRVRRQHQGDADQFPAERLAIEDA
                     IVAEADAIVAECPQDRTDLVGLYRADPRKIAVVPCGFDAGEFWPIARRFARHTLGIDA
                     DERVVVNIGRMVPRKGVDNVIRGVARLGRSFGVRARLLVVGGNSDVPDPALTPEIGRL
                     RAVADEERVAGRVTFTGRRSRELLKLYYSAADVFVTTPWYEPFGITPLEAMACGTPVI
                     GSAVGGIKHSVLDGVTGYLVPANEPDALAARLADLYASPGRAREFGRNAVRRVNASFT
                     WQRVSGSIASLYERVAGGPEALPGERWVAAA"
     gene            complement(3225..3725)
                     /locus_tag="VI319_13840"
     CDS             complement(3225..3725)
                     /locus_tag="VI319_13840"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEY6864974.1"
                     /translation="MSRYHIAQLNIARMRTPLDDPAMAGFVARLDEINALADGSPGFV
                     WRLKTDGGNATYLRPYDDDRILFNMSVWETVEHLRRFVYGTMHVEVMRRREAWFEKFA
                     GAFFALWWVPTGHVPSVDEGKQRLAHLDAHGPTQFAFTFRSVFQPDDAFQRGIDWSAF
                     KPCPAV"
     gene            3942..4286
                     /locus_tag="VI319_13845"
     CDS             3942..4286
                     /locus_tag="VI319_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864975.1"
                     /translation="MISCSTGGRVVYMTIRDPHSAEESISFLSALSRNPSVPDSALLL
                     VDTRSADGAFPEIEVALLGPKLGPCCAFVIGGHQAEHARDYGTRMQRGGGPAVEIFSD
                     LSKAYDWIGKYR"
     gene            complement(4283..4624)
                     /locus_tag="VI319_13850"
     CDS             complement(4283..4624)
                     /locus_tag="VI319_13850"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6864976.1"
                     /translation="MERVKDANPLYETEYRAFHAQRPGFRINELRISPTQKVPWHFHS
                     NVQDTFYVLEGEIRIFLREPKEEVRLKPGQTYAVPPRRPHLVLNGGQTSATFLVLQGI
                     GEYDFVPLVQP"
     gene            complement(4640..5383)
                     /locus_tag="VI319_13855"
     CDS             complement(4640..5383)
                     /locus_tag="VI319_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6864977.1"
                     /translation="MKSSPPPAAVADLAPTGKLRAAINFGNPVLASKDAGGEAKGVSV
                     DLARELAGRLGVPLAIVPYAAAGRVTDDAKKGVWDVAFVAIDPKRGADMAQSPPYVQI
                     EGAYLVRNDSPLRENAEVDREGTRVAVGKGSAYDLFLTRELKRATLVRAPTSPEVVDF
                     TLREKLDVAAGVKQQLEADAKRVGGVRLLPGRFMVINQAMASRKGAEAGERYVRDFIE
                     DMKASGFVARALERHRIEGAAVAPAGDAR"
     gene            complement(5527..5796)
                     /locus_tag="VI319_13860"
     CDS             complement(5527..5796)
                     /locus_tag="VI319_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY6864978.1"
                     /translation="MNIIVWLIVGGIVGWVASLVMRTDAQQGWLLNIVVGIVGAFLGG
                     LIFAPMFGTGTINEGEFSVGSLLVSLAGAVILLAIVNLVRRRAIR"
     gene            complement(5953..6135)
                     /locus_tag="VI319_13865"
     CDS             complement(5953..6135)
                     /locus_tag="VI319_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864979.1"
                     /translation="MKLKKFFIAHNAAVDREQLLAMFRAGLTSVLVRPGTAFWQIAGR
                     TLVGQHAYDAMIRARG"
     gene            complement(6272..6559)
                     /locus_tag="VI319_13870"
     CDS             complement(6272..6559)
                     /locus_tag="VI319_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864980.1"
                     /translation="MAKRAKKNERAKHGACELVIVADAPDTDIWLGNDHGHLIQRATG
                     TLKAKLVPGDYTVEFGLGSFPYPIRLEQPTRYAQSDLVANYGAPSAPATKR"
     gene            complement(6792..6959)
                     /locus_tag="VI319_13875"
     CDS             complement(6792..6959)
                     /locus_tag="VI319_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864981.1"
                     /translation="MAKPNYAFARRQREMAKKQKQEEKRRRKADTPDTQTPDTESVPV
                     PTTPKYEIPGG"
     gene            complement(7054..7875)
                     /locus_tag="VI319_13880"
     CDS             complement(7054..7875)
                     /locus_tag="VI319_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6864982.1"
                     /translation="MAEPANLEADVARAQSGDRAALEKVLRAVHKDVYNLALRFLWHP
                     QDAEDATQEILVRVLTGLGGFRGESAFRTWVYRVACNALLGARKRRMEERALTFEAFA
                     GDLEQGLSDAGDDDIENKLLLEEVKIGCTLAMLLCLDRDRRLAYIVGEILELDHDQGA
                     LVLDVTPDVFRKRLSRARAAIVAFMQARCGLVEPANACRCRKRVDAAVALGRVDLSQL
                     LFASSQRQAREFPQVLAEIRRLENTRRAAALYRSHPQYAPSNAFAAWLKGVLEAR"
     gene            complement(7868..8275)
                     /locus_tag="VI319_13885"
     CDS             complement(7868..8275)
                     /locus_tag="VI319_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864983.1"
                     /translation="MKTFDVQGIELRVPRAQAFAYIADPARLPAWTSAFASVADGRAL
                     LRTPAGEVEIGLGVDASAEHGTVDWRMSFPDGSVASAYSRLVETGRDACVFSFVLTPP
                     PVPLEQLEGALEAQSRTLAEELRTLKRVLERRG"
     gene            complement(8352..9311)
                     /locus_tag="VI319_13890"
     CDS             complement(8352..9311)
                     /locus_tag="VI319_13890"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6864984.1"
                     /translation="MPTRRDILKAGLAAGAAMHLPKSLAGASQPATPVAFAVPAGACD
                     CHTHIHGDPAQFPFFSGRVYTPETALPDEMAALHRSLHMQRVVIVTPSVYGTDNSATL
                     YGMKARGKDARGVAVIDDRTPESALDAMGKAGIRGIRLNLATGGQNDPSLGRKRFQDA
                     AERVKARGWHVQMYTNLAVVSSIKDLVLASPVPVVFDHFGGAQAHLGTGQPGFADLLE
                     LVRSGRAYVKISGAYRASKLAPDYEDAAPLAKALIAANPDRIVWGTDWPHPNSASGRP
                     KDEVTPLVQIDDGRLLNQLPVWAPDAAVRRKILVDNPTRLYQF"
     gene            9414..>9765
                     /locus_tag="VI319_13895"
     CDS             9414..>9765
                     /locus_tag="VI319_13895"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6864985.1"
                     /translation="MARFLVGVDIGGTFTDCVVLDRDGRITATKSSSTPPNFAEGMLA
                     AMGVAAERLGLTFEQFCKEIEVLTHGTTVGTNALIQRKGAKVGLITTKGHEDAIHIMR
                     GSRGVTSRDLRKVVH"
BASE COUNT         1440 a         3337 c         3562 g         1426 t
ORIGIN      
        1 gcagccggta cgggttctcg cggcagtgcg cgagccgcag cgggatgccg gcgagatagg
       61 cgaggtacgc cgcgggcagc gggttctggc tgtagacggt gaagatcacg gcggcgtcga
      121 agctcttcga cgcgagctgc acgatcatcg cgccgtccat ggtcgggccg tcgccggtcg
      181 ccttcatcca gggcgccgcg aacgtgatca cgtggtcgat ctcgcgcacg tacggggcga
      241 gctgcgcgcc ggcgggcgag gtgagcagcg tgacgcggcg gccgggcgcc gactccttca
      301 gcgcgcgtat cgccggcgtc gtcatgagca cgtcgccgat gttgtccagg cgcacggcca
      361 ggacgttctt cgcctcgggc caggggacgc gcctcatgcc gtgccgcaca tgagaacggg
      421 gccgacgcgc gcgtcgtcgc tcacgatcag cgccgcggcc tcgaacaggt ccgcggcgac
      481 cttgtcggga cggcgcccag gggtgaggtc ccactcggtc tcgttgccgt tgtcgatcag
      541 cacggtgcgg cagcccgcgc cacggcccgc ctcgacgtcg tcgagaatgt cgccgaccat
      601 ccacgagctc gcgaggtcga ggccgtgctc gcgcgcggcc cgggacagca gccccgacgc
      661 gggcttgcgg caggcgcaag gcactgcgta ctcgggcagc cgccccccgg gcaggtgcgg
      721 gcaatagtgg aagcccgaga gcggcacgcc gacgtcgcgc agcagctcgc gcagccgcga
      781 ttcgacgccg cccagcgcgt gctcggggaa aatgccgagg gcgacgccgg gctggttcga
      841 cacgacgacg atgcggtagc cgcggcgcac cagcaggtac agcccttcgg ccgcgcccct
      901 ggcgagcgtg atgcgcacgg gatcgacgtt gtaggcgacg tcgtcgatga gcgtgccgtc
      961 cttgtcgagg aacaccgcgc gatcggattc ccgggtcatg gccaggagct ctctcgcatc
     1021 gggatcacca tcacctcggg gatgacgctc tcctcgggca tggccaggag gaaggcgacc
     1081 gtggcggcga cgttcgccgg atcctgcagc acgcccgggt cgaggtcggg gaagcgatcg
     1141 agcaggaagg gcgtgcgcat gccgcccgcg accacggcgc tcaccttgat cccgagcggc
     1201 cgcagctcgg catgcagcgc gtggctgaag ccgagcaggc cccacttgct cgcgtggtag
     1261 gccgaggcgt tggcccaggc gcgcttcgcc gcggtcgagg cgatgttgac gatgtggccg
     1321 ccgccctggc gggccatgat cgcggccgcg cgcttcgcga gcaggaacgg cccgcgcagg
     1381 ttggtgcgga tcacgcggtc ccactgctcg accgagagct cctcgagcga cagcgtgtag
     1441 tcgacgccgg cgttgttcac cagcgcgtcg aggcgcccga ggcgctcgag cgtctcggcg
     1501 agggcgcgct cgcacgggtc ctcgtcgccg acgtcgacgc ggatcgcgca gccgccgacc
     1561 tcggccgcga tcgcctgggc gcgcgcgagg tcgagatccg cgaccgcgac ttgcgcgccg
     1621 tcctgcgcgc aggcgcggca gatcgcggcg ccgaggccgc gcccgccgcc cgtgacgaag
     1681 acgaccttgc cttcgaggcg gccgacgctc atgcggcggc cacccagcgc tcgccgggga
     1741 gcgcctcggg cccgcccgcc acgcgctcgt acagcgaagc gatcgacccc gacacgcgct
     1801 gccaggtgaa cgacgcgttc acccggcgca ccgcgttgcg gccgaactcg cgcgcgcgcc
     1861 ccgggctcgc atacaggtcg gcaagccgcg cggcgagtgc gtcgggctcg ttcgccggta
     1921 cgagatagcc ggtgacgccg tcgagcacgc tgtgcttgat gccgccgacg gccgagccga
     1981 tcaccggcgt gccgcacgcc atcgcctcga gcggcgtgat gccgaacggc tcataccacg
     2041 gcgtcgtgac gaagacgtcc gcggcgctgt aatagagctt cagcagctcg cggctgcgcc
     2101 ggccggtgaa agtgacgcgc ccggcgacgc gctcctcgtc cgccaccgcg cgcagccgcc
     2161 cgatctcggg cgtgagcgcg ggatcgggaa catcggagtt gccgccgacg acgagcagcc
     2221 gcgcccgcac gccgaagctg cggcccaggc gcgcgacgcc gcggatcacg ttgtccacgc
     2281 ctttgcgcgg caccatgcgg ccgatgttga ccacgacgcg ctcgtcggcg tcgatgccga
     2341 gcgtgtgccg ggcgaagcgg cgcgcgatcg gccagaactc cccggcgtcg aagccgcacg
     2401 ggacgacggc gatcttgcgc gggtcggcgc gatacaaacc gaccaggtcg gtcctgtcct
     2461 gcgggcactc ggcgacgatg gcgtcggcct cggccacgat cgcgtcctcg atggcgaggc
     2521 gctcggccgg gaactggtcg gcgtcgccct ggtgctgccg gcgcacgcgc ccgagcgcgt
     2581 gaaaggtgac gacgaacggg atgtcgaagc gctgcttcag cttgatcgcg acgagccccg
     2641 acatgaagaa gttcgcgtgc acgaggtcgt agccgccctc gcgccggcag aacgagcaga
     2701 cgcaggcgga gaactcctcc atcagcggca gcaggtcttc cttgcgcacg tatcgcgcgg
     2761 gcccggcggg cacgtggatc acgcgcacgc cgcgcgccca ctcctgcacc agcggctggt
     2821 cgggcgcgtc gcgccgcgtg aacacgtcga ccgcgtagcc gagcgcggcg agatggcgcg
     2881 cgagctgcgc gacgtagatg ttctgcccgc ccgagtcgac gcctcccgcc gcggcgagcg
     2941 gcgaggcgtg atcgctgacg agcgcgattt tgcggagcat tgggggagtc ctctcgacct
     3001 gctccgtcgg aaaaagcaaa tccagtgcca aacgcggttt gtcgcgcggt gcgcgcgccg
     3061 ccctctgggc gagagcccgc ttcatgcgtg gatagagcgg cgcgatgcgc ttctacgggg
     3121 taagaattac gatcgcaccg catttcttac atcccagaaa gcgcgcatat gacggcatga
     3181 gccgcccggc gcgcgcttgc gccgctcgaa ctaagcgcgc gcggtcagac cgcgggacag
     3241 ggcttgaagg cggaccagtc gatgccgcgc tggaacgcgt cgtcgggctg gaacacgctg
     3301 cggaaggtga acgcgaactg cgtcgggccg tgcgcgtcga gatgcgcgag ccgctgcttg
     3361 ccctcgtcga cgctcggcac gtggccggtc ggcacccacc agagcgcgaa gaaggcgccg
     3421 gcgaacttct cgaaccaggc ctcgcgccgg cgcatgacct cgacgtgcat cgtgccgtag
     3481 acgaaccggc gcaggtgctc gaccgtttcc cagaccgaca tgttgaagag aatccggtcg
     3541 tcgtcgtagg gccgcaggta ggtcgcgttc ccgccgtcgg tcttcaggcg ccagacgaac
     3601 ccgggagagc cgtcggcgag cgcgttgatc tcgtccaggc gcgcgacgaa gcccgccatc
     3661 gcgggatcgt cgagcggcgt gcgcatgcgg gcgatgttga gctgggcgat gtggtagcgg
     3721 ctcatgggtt ctccgggcgt cgagcgggag tctacgccgg gtcgcgcgcg cgcggagcgg
     3781 ccgctgtcgc gcggccgacg gcggtggcag ccgatccgaa gcgcgccggc gccgcgctcc
     3841 cggaattcgt gagattgatc acgcgaaatc gcggcgcagc gcatggcatc gctcttgctg
     3901 ttccttcgcg caaggcaaga acgaaaaaca aggagaatcc cgtgatctca tgctcgacgg
     3961 gaggccgcgt ggtctacatg acgatccgcg acccgcattc ggccgaagag tcgatctcgt
     4021 tcctgagcgc gctcagccgc aatccctcgg ttccggactc ggcgctgctg ctggtcgaca
     4081 cgcgctccgc ggacggcgcg ttccccgaga tcgaagtcgc gctgctcggg ccgaagctcg
     4141 gcccgtgctg cgccttcgtc atcggcggcc accaggccga gcacgcgcgc gactacggga
     4201 cgcgcatgca gcgcggcggc gggccggccg tcgagatctt cagcgacctc tccaaggcct
     4261 acgactggat cgggaagtac cgctagggct gcaccagcgg cacgaagtcg tactcgccga
     4321 tcccctggag cacgaggaag gtcgccgagg tctgcccgcc gttcaggacc aggtgcgggc
     4381 gccggggcgg cacggcgtag gtctgccccg gcttgaggcg cacctcctcc ttcggctcgc
     4441 gcaggaagat gcggatctcg ccttcgagca cgtagaacgt atcctgcacg ttgctgtgga
     4501 agtgccacgg caccttctgc gtgggcgaga tcctgagctc gttgattctg aagcccgggc
     4561 gctgggcgtg gaacgcccgg tactcggtct cgtagagcgg gttggcgtct ttcactcgtt
     4621 ccatgggagc tcctttcggc tagcgcgcgt caccggccgg cgcgaccgcg gcgccctcga
     4681 tgcggtggcg ctcgagggcg cgcgcgacga agcccgaggc cttcatgtcc tcgatgaagt
     4741 cgcgcacgta gcgctcgccg gcctcggcgc ctttgcgcga ggccatcgcc tggttgatca
     4801 ccatgaagcg gccgggaagg agcctcacgc cgccgacgcg cttcgcgtcg gcctcgagct
     4861 gctgcttcac gccggcggcg acgtcgagct tctcgcgcag cgtgaagtcg accacttcgg
     4921 gcgacgtggg cgcgcgcacg agcgtcgcgc gcttcagctc gcgcgtgagg aacaggtcgt
     4981 aggcgctgcc cttgccgacg gcgacgcgcg tgccctcgcg gtcgacctcg gcgttctcgc
     5041 gcagcggcga gtcgttgcgg accaggtacg cgccctcgat ctgcacgtac ggcggcgact
     5101 gcgccatgtc ggcgccgcgc ttcgggtcga tcgcgacgaa ggcgacgtcc cagacgccct
     5161 tcttcgcgtc gtcggtcacg cggcccgcgg ccgcgtaggg cacgatcgcg agcggcacgc
     5221 cgaggcggcc ggcgagctcg cgcgcgaggt cgaccgacac gcccttcgcc tcgccgcccg
     5281 cgtccttcga cgcgagcacc gggttgccga agttgatcgc ggcgcgcagc ttgccggtcg
     5341 gggcgagatc ggcgacggcg gcgggcggcg gggaggattt catgggcggg gcggcacagg
     5401 cggccaggac gatcagcagc aaaagcgctg agacggcgcg catcgggttg ctccggaatc
     5461 ggctaggcgc gcagtttatc gcttccgggg aaacctatcc cggcggcaag ggcgagcctc
     5521 cccgggtcag cgtatcgccc gccgccggac gaggttgacg atcgcgagca ggatcaccgc
     5581 gcccgcgagc gacacgagga gggaccccac gctgaactcg ccctcgttga tggtgcccgt
     5641 cccgaacatc ggcgcgaaga tcaggccgcc gaggaaggcg ccgacgatgc cgacgacgat
     5701 gttcaggagc cagccctgct gcgcgtcggt gcgcatgacg aggctcgcga cccagccgac
     5761 gatgccgccg acgatcagcc agacgatgat gttcataccg gtcttgcctc cttccgatga
     5821 ggacggatga gcgcaagcaa gcgcggtgcc gatggtccga acggatcgga cccggcgccg
     5881 gtgtgaggcg atggccccgg acgggccgcc gcggcgctga aaaagctgcg cggcccggaa
     5941 attcttacac gctcagccgc gcgcgcggat catcgcgtcg tacgcgtgct ggccgacgag
     6001 cgtgcgcccg gcgatctgcc agaacgcggt gcccggccgg accaggacgc tggtcagccc
     6061 ggcgcggaac atggcgagca gctgctcgcg gtcgacggcg gcgttgtgcg cgatgaagaa
     6121 ctttttcagc ttcatggagc ttccctccca ggcgtcgcgg atgcgacgac gagagagaag
     6181 caacccgcgg gcccagtgac aaacgtctcg ccagggtgcc gcggcgcggc gcggccgaga
     6241 actacttggc gccggcgggc cggggcgctc gtcagcgctt cgtcgccggc gcggaaggag
     6301 cgccgtaatt cgccacgagg tcggactgcg cgtagcgggt cggctgctcg agtcggatcg
     6361 gatacgggaa ggagccgagg ccgaactcga ccgtgtagtc accgggaacg agcttcgcct
     6421 tcagcgtccc ggtcgcgcgc tggatcaggt gaccgtgatc gttgccgagc cagatgtcgg
     6481 tgtcgggcgc gtcggcgacg atgaccagct cgcacgcgcc gtgcttcgcc ctctcgtttt
     6541 ttttcgctcg cttcgccatc tcggatcgag gcgcggggcg gtcccggcgc ctttgttcag
     6601 cgatagggct tcgggtactt gggcgcgaag ggctcgccgg gacggacgct gactacgtag
     6661 cggcggcaat tgatgcaccg gagccagctt tggtcgctgg tgccgtcgct gcgcgtgacg
     6721 acatgcgact cggagacggt gccgccgcag aggggacagc agaacttgcg ttcgggcatg
     6781 gcgaccgcgg ctcagccgcc agggatctcg tacttgggcg tcgtcgggac ggggacgctc
     6841 tccgtgtcgg gtgtctgcgt gtcgggggta tcggccttgc gccggcgctt ctcttcctgc
     6901 ttctgcttct tggccatctc gcgctggcgt cgtgcgaagg cgtagttggg cttggccatg
     6961 ggatcggggt ccggaaggtt gcggatgctc ccagtgtaag cccgtttgcc cgggaccggc
     7021 agccggtcgc gaaagcgcgt cccgcgcccc ggcctagcgc gcttcgagca cccccttcag
     7081 ccaggcggcg aacgcgttcg acggcgcgta ctgaggatgc gatcgataca gcgccgccgc
     7141 gcgccgcgtg ttctccagcc gccggatctc ggcgagcact tgcgggaact cgcgcgcctg
     7201 ccgctgcgac gacgcgaaga gcagctgcga caaatcgacg cggccgagcg caaccgccgc
     7261 gtcgacgcgc ttgcggcacc ggcacgcgtt ggcgggctcg accagcccgc agcgcgcctg
     7321 catgaacgcg acgatcgccg cgcgcgcccg cgacaggcgc ttgcggaata cgtcgggcgt
     7381 gacgtcgagg accagggcgc cctggtcgtg gtcgagctcc aggatttcgc cgacgatgta
     7441 ggcgaggcga cggtcgcggt cgaggcagag cagcatggcg agcgtgcagc cgatcttcac
     7501 ctcctcgagc agcagcttgt tctcgatatc gtcgtcgccg gcgtccgaga ggccctgctc
     7561 gagatccccg gcgaaggcct cgaaggtcag ggcgcgctct tccatgcgcc gcttgcgcgc
     7621 gccgagcagc gcgttgcagg cgacgcggta gacccaggtg cggaaggcgc tctcgccccg
     7681 gaagccgccg aggccggtga gcacgcgcac gaggatctcc tgcgtcgcgt cctcggcgtc
     7741 ctgggggtgc cagaggaagc gcagcgcgag gttgtacacg tccttgtgca cggcgcgcag
     7801 cactttctcg agcgccgcgc ggtcgcccga ctgcgcccgc gcgacgtcgg cctcgagatt
     7861 cgccggctca gccacggcgc tcgagcacgc gcttcagcgt gcgcagctcc tcggcgagcg
     7921 tgcgcgactg ggcctcgagc gcgccttcga gctgctcgag cggcacgggc ggcggcgtca
     7981 gcacgaagct gaagacgcac gcgtcgcgcc cggtctccac gagccgcgag taggcgctcg
     8041 cgacgctgcc gtcgggaaag ctcatgcgcc agtcgaccgt gccgtgctcg gccgaggcgt
     8101 cgacgccgag gccgatctcg acctcgcccg cgggcgtgcg cagcagcgcg cgcccgtccg
     8161 cgaccgatgc aaaggcgctg gtccaggcgg gaagccgcgc cgggtcggcg atgtacgcga
     8221 acgcctgcgc gcgcggaacg cgcagctcga tgccctggac gtcgaaagtc ttcatcggag
     8281 ctccttcggt tgcggcccgc cgatcgcggg cctcgtcatg cttagactcg ccgaagatgc
     8341 tccgtgtgac gctaaaactg gtagagccgc gtcgggttgt cgaccaggat cttcctgcgg
     8401 acggcggcgt cgggggccca gacgggcagc tggttgagca ggcgcccgtc gtcgatctgg
     8461 acgagcggcg tgacctcgtc cttgggtcgc cccgaggccg agttcgggtg cggccagtcg
     8521 gtgccccaga cgatgcggtc ggggttcgcc gcgatcagcg cttttgcgag cggcgccgcg
     8581 tcttcgtagt cgggcgcgag cttcgaggcg cgatacgcgc ccgagatctt cacgtacgcg
     8641 cgccccgagc gcaccagctc gagcaggtcg gcgaagccgg gctggcccgt cccgaggtgc
     8701 gcctgcgcgc cgccgaagtg gtcgaacacc acgggcacgg gcgaggcgag caccaggtcc
     8761 ttgatcgacg agaccaccgc gaggttggtg tacatctgca cgtgccagcc gcgcgccttc
     8821 acgcgctcgg ccgcgtcctg gaagcgcttg cggccgagcg acgggtcgtt ctgcccgccg
     8881 gtcgcgagat tcaggcggat gccgcggatg cccgccttgc ccatcgcgtc gagcgcgctc
     8941 tcgggcgtcc ggtcgtcgat caccgccacg ccgcgcgcgt ccttgccgcg cgccttcatg
     9001 ccgtagagcg tcgccgagtt gtccgtgccg tacaccgacg gcgtgacgat caccacgcgc
     9061 tgcatgtgca gcgagcggtg cagcgccgcc atctcgtcgg gcagcgccgt ctcgggcgtg
     9121 tacacgcgcc cggagaagaa cgggaactgc gcgggatcgc cgtggatgtg cgtgtggcag
     9181 tcgcaggcgc ccgcgggaac ggcgaaggcg accggcgtcg cgggctgcga ggcgccggcg
     9241 agactcttgg gaagatgcat ggcggctccg gcggcgaggc cggccttgag gatgtcgcgg
     9301 cgggtgggca tgggtgctcc tgcggcgcgg tgggggaaac cactttacac gaaccgactc
     9361 cgtggaaaaa taggctacca tcccgccctc acccccgcag gatctacaag aaagtggccc
     9421 gttttctcgt cggcgtcgac atcggcggta cgtttacgga ctgcgtcgtg ctcgaccgcg
     9481 acggccgcat caccgcgacc aagtcctcct cgacgccgcc gaatttcgcc gaggggatgc
     9541 tcgcggcgat gggcgtcgcg gcggaacgcc tgggcctgac cttcgagcag ttctgcaagg
     9601 agatcgaggt gctgacgcac ggcacgaccg tcggcaccaa cgcgctgatc cagcgcaaag
     9661 gcgccaaggt cgggctgatc acgacgaaag gccacgagga cgcgattcac atcatgcgcg
     9721 gctcgcgggg ggtgacctcg cgcgatctcc ggaaggtggt gcact
//
LOCUS       DATDMW010000332         3400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2340295, whole genome shotgun sequence.
ACCESSION   DATDMW010000332 DATDMW010000000
VERSION     DATDMW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340295"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VI319_13900"
     CDS             <1..660
                     /locus_tag="VI319_13900"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY6864986.1"
                     /translation="MFQLAAETRLGLSYRSQMKYKVEGDVTFSNVPSAATFNAIDSTG
                     ALAAKFANGRVTLDLKLPDSFSAALSHKIDARWTLLSDVTWTGWSSIPQLRILRADGS
                     ELSNTPENFKDTWRVGVGTDYRYNDAWSSKMGLAYDQSPVNNTDRTARLPDNDRYWVS
                     VGGQYRVSNAGTLDFGYSHLFVKNTSINQPGTNAQVNGQVVGSYKASIDIIGIQYSQS
                     F"
     gene            complement(657..2270)
                     /locus_tag="VI319_13905"
     CDS             complement(657..2270)
                     /locus_tag="VI319_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6864987.1"
                     /translation="MADANQPPPLAGYDAWSQDRQLVDAVEREGGGWIATEAKAMGEL
                     VGSERMQALARDANRHEPELRTHDRTGERIDAVDYHPAYHELMALAFGAGLHSLPWTS
                     ARAGRWVARAALYYLWNQGENGVACPVTMSFAAARLLAVDAALEAEWKPKLVANGYDP
                     RPLPLAQKRAVTVGMAMTERQGGSDLRSNETVAAPAGAGEYRLEGHKWFCSAPMSDAF
                     FTLARTESGLSCFFVPRSLPDGARNPFHIQRLKDKCGNRSNASAEIEYHGTRARRVGD
                     EGRGIATLIEMAHLTRFDIVIASAGMMRAGLVQALHHAAHRRAFGKRLAEHALMQNVL
                     ADLALETEAAMLLAFRLARAFDRAGDDEGERLLSRLVTPVAKYWLCKRNPAVALEAME
                     CLGGNGYVEEAPLARLYRDAPVNGIWEGSGNVICLDVLRSIQREPETLEALIREIASA
                     GNDFQTLAGRIVQQLQPWDTIERRARRVVEMLAVALQASLMARFAAGDAAEAFRASRL
                     GGDWGRAFGTLPAGANAEAIVRRAGWTGD"
     gene            complement(2270..3241)
                     /locus_tag="VI319_13910"
     CDS             complement(2270..3241)
                     /locus_tag="VI319_13910"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864988.1"
                     /translation="MTRRIVQVLFFAAAFCFEFAAGSSFAQESAYPGKGPVEITVLFP
                     AGSSADVTARVLADGMAKQLGTQVLVVDRPGAGGAIGYRHVAAQKPDGYSLVWNSNSI
                     STTYHSGQLPFDYRAFDAVARVLVEAPVVAVRADSKWRTLGDLIADAKARPKMLTVGN
                     SGAGSHTHISSVALFRTAGAEVTDVPFPAAQTVPSLLGGHVDALVQLPAALSSYVKNG
                     QVRLLAALTPSRDPALPGVPTAREQGVDVSLEAWRGIAVPAGTPKPVVAALESAIRKT
                     ATSAEFAQASEKLGVTPAFMPAAEFGALIAREDATLARLMEIIGLKK"
BASE COUNT          520 a         1221 c         1167 g          492 t
ORIGIN      
        1 atgttccagc tggccgcgga gacgaggctg ggcctgtcgt accgctcgca gatgaagtac
       61 aaggtcgaag gcgacgtgac attcagcaac gtcccctcgg ctgcgacctt caatgcgatc
      121 gattccaccg gtgcgctcgc cgccaagttc gcgaacggca gagtaacgct ggacctcaag
      181 ctgcccgatt cattctccgc cgcgctgagc cacaagatcg acgccaggtg gacgctgctt
      241 tccgacgtga cgtggaccgg gtggtcctcg atcccgcagc tgaggatcct gcgcgctgac
      301 ggcagcgagc tcagcaacac gcccgagaat ttcaaggaca cgtggcgtgt cggggtcggc
      361 acggactatc gctacaacga cgcctggtcg agcaagatgg gcctcgccta cgaccagagc
      421 ccggtgaaca acaccgatcg caccgctcgt ctgcccgaca acgaccggta ctgggtctcg
      481 gtcggggggc aatatcgcgt ttcgaatgca ggaacgctcg acttcggcta ctcgcacctg
      541 ttcgtcaaga acacgtcgat caaccagccg ggaacaaacg ctcaggtgaa cggccaagtc
      601 gtcggcagct acaaggcgtc gatcgacatc atcggcatcc agtacagcca gagcttctag
      661 tcacccgtcc agcctgcccg ccgcacgatc gcttccgcat tcgcgccggc gggcagcgtc
      721 ccgaacgcgc ggccccagtc gccgccgagg cgcgaggcgc ggaacgcctc cgccgcgtcg
      781 ccggccgcga agcgcgccat caggctcgcc tgcagcgcga ccgcgagcat ctcgacgacg
      841 cgccgcgcgc gacgctcgat cgtgtcccac ggctgcagct gctggacgat gcggccagcg
      901 agcgtctgga agtcgttgcc ggccgaggcg atctcgcgga tgagcgcctc gagcgtctcg
      961 ggctcgcgct ggatcgagcg cagcacgtcg aggcagatga cgttccccga gccttcccag
     1021 atgccgttca cgggggcgtc gcggtacagc cgcgcgagcg gcgcctcttc gacgtagccg
     1081 ttgccgccga ggcactccat cgcctcgagc gcgaccgccg ggttgcgctt gcacagccag
     1141 tattttgcga ccggggtcac gagccgcgag agcagccgct cgccctcgtc gtcgcccgcg
     1201 cggtcgaacg cgcgcgcgag gcggaaggcg agtagcatcg cggcctcggt ctccagcgcg
     1261 agatcggcga gcacgttctg catgagcgcg tgctcggcga gccgcttgcc gaacgcgcgg
     1321 cggtgcgccg cgtggtgcag cgcctgcacc aggcccgcgc gcatcattcc cgccgacgcg
     1381 atcacgatgt cgaagcgcgt gaggtgcgcc atctcgatga gggtcgcgat gccgcggccc
     1441 tcgtcgccga cgcggcgggc gcgcgtgccg tggtactcga tctcggcgga ggcattcgag
     1501 cggttgccgc atttgtcctt caggcgctgg atgtggaacg gattgcgggc gccgtcgggg
     1561 agcgagcgcg ggacgaagaa gcagctgagg cccgactcgg tacgcgcgag cgtgaagaag
     1621 gcgtcgctca tcggcgccga gcagaaccac ttgtggccct cgagccggta ttcgccggcg
     1681 cccgcgggcg cggcgacggt ctcgttcgag cgcaggtccg agccgccctg gcgctcggtc
     1741 atcgccatgc cgacggtcac cgcgcgcttc tgcgcgagcg gcagcggccg cggatcgtat
     1801 ccgttcgcga cgagcttcgg cttccactcg gcctcgagcg ccgcgtcgac ggcgagcagg
     1861 cgcgccgccg cgaacgacat cgtcaccggg caggcgacgc cgttctcgcc ctggttccac
     1921 aggtagtaga gcgccgcgcg cgctacccag cgccccgcgc gcgccgacgt ccacgggagc
     1981 gaatgcagcc cggcgccgaa ggcgagcgcc atcagctcgt ggtaggccgg gtgatagtcg
     2041 accgcgtcga tgcgctcgcc ggtgcggtcg tgcgtgcgca gctcgggctc gtgccggttc
     2101 gcgtcgcgcg cgagcgcctg catgcgctcg ctgccgacga gctcgcccat ggctttcgcc
     2161 tcggtcgcga tccatccgcc gccctcgcgc tcgaccgcgt cgacgagctg ccggtcctgc
     2221 gaccaggcgt cgtagcccgc gagcggcggc ggctggttgg cgtcggccac tatttcttca
     2281 gcccgatgat ttccatcagg cgcgcgagcg tcgcgtcctc gcgtgcgatc aacgcgccga
     2341 actcggcggc gggcatgaac gcgggcgtga cgccgagctt ctcgctcgct tgcgcgaact
     2401 cggcgctcgt ggcggtcttg cgtatcgccg actcgagcgc ggcaacgacc ggcttcggcg
     2461 tcccggccgg caccgcgatg ccgcgccagg cctcgagcga aacgtcgacg ccctgctcgc
     2521 gcgcggtcgg cacgccgggc agcgccggat cgcgcgaggg cgtcagcgcg gcgagcagac
     2581 gcacctgccc gtttttgacg tagctggaaa gcgccgcggg aagctgcacc agcgcgtcca
     2641 cgtgaccgcc gagcaggctc ggcaccgtct gcgcggccgg gaagggaaca tcggtcacct
     2701 cggcgcccgc cgtccggaac agcgcgaccg acgagatatg cgtgtggctg cccgcgcccg
     2761 agtttccgac ggtcagcatc ttcggccgcg ccttggcatc ggcgatcaga tcgccaagcg
     2821 tgcgccactt cgaatcggcg cggacggcga ccaccggcgc ctccaccagc acgcgcgcca
     2881 ccgcgtcgaa ggcccggtag tcgaacggca gctgccccga gtggtacgtg gtcgagatcg
     2941 agttcgagtt ccacaccagc gagtagccgt cgggcttctg cgccgccacg tgccggtagc
     3001 cgatcgcgcc gcccgcgccg gggcgatcga cgaccagcac ctgcgtgccg agctgcttcg
     3061 ccatgccgtc cgcgagcacg cgcgccgtca cgtcggccga ggatccggcc gggaagagca
     3121 ccgtgatctc gaccgggcct ttgccggggt aggcggactc ctgcgcgaaa gaagatcccg
     3181 ccgcgaattc gaagcaaaaa gcggcggcga agaacaggac ttgcacgatg cgccgggtca
     3241 tacgatctcc tcagtcgttc gccatgatct ccgcaacttc cacgcgacgc gcggtgcgcg
     3301 cggacagcac gcccgcccgc acgaccgcga gcgagcgcgt cgcgcgctcg ccgtccatct
     3361 ccggcgcgcc ctcgccgcgc acggcgcgcg cgaactcctc
//
LOCUS       DATDMW010000333         9065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_471947, whole genome shotgun sequence.
ACCESSION   DATDMW010000333 DATDMW010000000
VERSION     DATDMW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471947"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VI319_13915"
     CDS             complement(<1..672)
                     /locus_tag="VI319_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6864989.1"
                     /translation="MADPTRIRTTIPARLDSLPWSRWHWRVVVALGITWLLDGLEVTV
                     VGALSAVLQRADTLGLSAAEVGWAGSAYIAGAVAGALFFGRLADRLGRKRLFLVTLAV
                     YVAATVLTGLTWDFASFALCRLLTGFGIGGEDAAINSAIDELIPARVRGVVDLGINGT
                     FWVGAALGAGLSLALLDPDWIGARLGWRLAFGLGAVLGVAILIVRRHVPESPRWLITH
                     GHAERA"
     gene            755..1735
                     /locus_tag="VI319_13920"
     CDS             755..1735
                     /locus_tag="VI319_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6864990.1"
                     /translation="MRIEKPLSILGLALAAAAGACSAAAQEWPVKPVHVIVPYVAAGA
                     ADIFGRTLAQKLGESLGETFVVENRPGANGGIGSELVAKAAPDGYTLLATASGPIVVN
                     PVLYAKVPYDPLRDFAPVAQATVYQYVLVTLASSPFKSIADIVAAARAKPGALTFGST
                     GIGGGNHLAGELLALSTGTQLTHVPYKGSAAALADLLGGQLSFMFDTVITSVPQIRAG
                     KLRAFAVSSAKRASSLPDVPTMQEAGIAGFDISQWQGVLAPAGTNRAIVDKLNAGIRK
                     ALAAPDVRERLVTQGGNEIVTGSPEDFAALIRSDLRQYAKLIRDAKIPAQ"
     gene            1732..2790
                     /locus_tag="VI319_13925"
     CDS             1732..2790
                     /locus_tag="VI319_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY6864991.1"
                     /translation="MRILVLPGDGIGPEITAATIAVLDRANALFDLGLEWHLEEIGFR
                     TLKKEGTTLPQRVLDMARKVDGVILGPLSTYEYPPRERGGLNPSGEMRVKLDLYANIR
                     PAKSRLGVGLTGKPVDLVIYRENTEGFYADRNMHLGVGEFMPTEDMALSVRRVTAKCC
                     SRIARRAFEAAMLRRKKVTAVHKANVFKISDGLFLREVRKVAREFPEVQLEEVIVDAM
                     AALLLRDPQRFDVIVAENMYGDILSDEASELSGGLGLGGSINAGDDICVAQAQHGSAP
                     DIAGKDRANPTSLILSAAMLLDWIGRRSGTEALGAAAQYIEAAVDKVLRDPATRTADL
                     GGTLGTQAFAKAVAETLG"
     gene            complement(2787..4814)
                     /locus_tag="VI319_13930"
     CDS             complement(2787..4814)
                     /locus_tag="VI319_13930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6864992.1"
                     /translation="MRSKSLKTRITVAVALALVGVVALVSLAQAYFAKAQMQRLLQAQ
                     QFTLVSMVAQDIDEKIDIRVKSLASVARVLPAEHLSSARLVRADLDNQPDLESQFDAV
                     AAILPSGQVLATYPDALSARRLNLGDRSWFRQVVRNGKPLISEPLRSRLTGDPQIVFA
                     APIFGRRGEVAGVLSGTLNLFKPNFLGGIGAASVGRTGSFALFTRDRTIVMSRDRDRI
                     MTQGPAPGVSPQFDRATGGVEGSEENVTSRGLHAIFSSKQLQTVPWVLVAALPVEEAY
                     APVVEAQQRVLLTALVFVALLAPGLWLGMRRLLGPLTVLHDVIRRIRAGSGDVPQVLV
                     RRSDEVGQLAADFEAMVRERGAAERALLESQRRLSLAMEGSQLALFDWDIASGAVFLS
                     ERWSSLLGGAAVPLRTTFAELAQSVHPEDFPRMRAALHEVLKGARRHFFAEHRVRTPA
                     GQWLWIQSHGEVTARDAAGRALRMVGTNADITERKRAEQALLDSRAELERAARYDALT
                     GLPNRNLLDDRLAQALARSRRSGHPVALLYVDLDGFKAVNDELGHAAGDALLKQFAAH
                     LSSCVRESDTVARLGGDEFVVLLDGMNRADDAGAVACKIVDSLPAGIDVGAGRARVTA
                     SIGIAIADGDTSPDDLLRRADEALYEAKRAGRNRVHLAPPAHAARARERPL"
     gene            5092..7302
                     /locus_tag="VI319_13935"
     CDS             5092..7302
                     /locus_tag="VI319_13935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6864993.1"
                     /translation="MRHFVPAAALALALFVRWLLDPWLHDAWAMPLVLGAVAAAVWYG
                     GWVQAAMVALAGYLFSDFMFMTPRGSLSITSVAGLLGFALYSASSALIIALGHRARRE
                     RREARASAQLLGRTLSGARVAAWEWDVTSDVLRLSDRTPDIFGAGREFRPSRRLLAYI
                     HPDDRQTHVRAVRAALCGGDKIDYGFRVVRPDGRVVWFESRGDVSRDASGRAIRIRGI
                     VIDVTERSLAHRALRETEQRLQIAQDLAPHGYDVLESVRAEGRIVDFRVAYINATGAA
                     LAKRDPEDLVGTRVLDVFPAVASTVLFERLHETCETGRGSDFEVHYPDGNGGTWFRYL
                     LAKVDSSVATSFIDITANKHLEEELRRRADALKEADRRKNEFLAVLAHELRNPLAPVS
                     TSLQILKRIGGDSRTARTLDVMERQLAQIVHLIDDLLDISRIEQGKTTLHRSVVQLQE
                     ALAQGIDTAEPHIRAKSHELTVDMTPVPLHVHADPIRIAQVVANLLNNAAKYTHSHGR
                     IRLALRSEAGYAVIIVADNGIGIPHDKLKSIFDLFVQVDRASDNAGGGLGLGLTLAKN
                     LIEIHGGTITATSEGLGKGSTFVVRLPLVVEPALERRPRQDARAAAAHPARRILVVDD
                     NQDAAETLAQLLRLSGDEVRTTDNGEDALAIGPEFRPHVAILDLGMPDIDGYELAQRV
                     RRQRWGSGVLLVALSGWGQEEDKVRSRAAGFDHHLTKPVSPGDLEQVLEHYAAI"
     gene            7371..7862
                     /locus_tag="VI319_13940"
     CDS             7371..7862
                     /locus_tag="VI319_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864994.1"
                     /translation="MTTHTLHHGGESLPRWIVVGFVSGAVSVLVFHQGAAALLHALEL
                     TPRAPYSMTPTPPWGVPQVWSLAFWGGVWGVVFAALLARLDGAWLVVVSTVLGAVLPT
                     LVAWFVVAPLKGQPAAAGWAPAAMMIGPIVNAAWGLGTGLGLALAGRTHARIEADRSP
                     SRN"
     gene            complement(7849..8322)
                     /locus_tag="VI319_13945"
     CDS             complement(7849..8322)
                     /locus_tag="VI319_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864995.1"
                     /translation="MIRGRALLLFACALAWPGPAHALRNEATPTSFRLTLSEDFVHAK
                     GATLLAGRYGGEWGLRAGAWLNADDMEPKPHWMAGFDHVWTLGKWRPGIGFAWIDRTT
                     NVNGTHWLFDASLAYEINRRLFVEYRHHSHGSKLGIQRSVQNGGWNLIGIGLSFD"
     gene            complement(8341..8541)
                     /locus_tag="VI319_13950"
     CDS             complement(8341..8541)
                     /locus_tag="VI319_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864996.1"
                     /translation="MAKRKQTKAKTTGGAEPPAPGQPNHDERMLDESIEESFPASDPA
                     TSSRPGSTLGRRYATTRKKRRG"
     gene            8693..>9065
                     /locus_tag="VI319_13955"
     CDS             8693..>9065
                     /locus_tag="VI319_13955"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY6864997.1"
                     /translation="MSEDMYRVGISGSYGGMNLGDEAILQSIVAQLRRDLPVEITVFS
                     RNAEDTRKRHKVERAVPVRKLSRAEVTPEIERLDLLLLGGGGILYDRDAETYLREVAL
                     AGELGVPVLLYAISAGPLVTPA"
BASE COUNT         1346 a         3278 c         3152 g         1289 t
ORIGIN      
        1 ggcgcgctcg gcgtgaccgt gcgtgatcag ccagcgcggg ctctcgggca cgtgccggcg
       61 cacgatcagg atcgcgacgc cgagcaccgc gcccaggccg aacgcgagcc gccagccgag
      121 gcgcgcgccg atccagtccg ggtcgagcag cgcgagcgag aggccggcgc cgagcgccgc
      181 gccgacccag aacgtgccgt tgatgccgag atcgacgacg ccgcgcacgc gtgccgggat
      241 gagctcgtcg atcgccgaat tgatcgccgc gtcctcgccg ccgatgccga agcccgtcag
      301 caggcggcac agcgcgaagc tcgcgaaatc ccaggtgagg ccggtgagca ccgtggcggc
      361 gacgtagacc gccagcgtca cgaggaacag gcgcttgcgt ccgaggcgat cggcgagccg
      421 tccgaagaag agcgcccccg cgaccgcgcc cgcgatgtac gccgagccgg cccacccgac
      481 ctcggcggcg gagagcccga gcgtgtcggc gcgctgcagc accgcgctca gcgccccgac
      541 caccgtgacc tcgaggccgt cgagaagcca ggtgatgccg agcgcgacga cgacgcgcca
      601 atgccagcgc gaccagggaa gcgagtcgag ccgagccgga attgttgttc gaatgcgggt
      661 gggatcggcc atggaggcgg ctgcgtaccc aacgacgcta ctatgaccac gccgggccgg
      721 cgcagttcgc accggtgttg cataatgcgg ctccatgaga atcgaaaaac cgctatcgat
      781 ccttggactt gcgctcgccg ctgccgcggg cgcctgttcc gccgcggccc aggaatggcc
      841 ggtgaagccg gtgcacgtga tcgtgcccta tgtcgcggcc ggcgccgccg acatcttcgg
      901 gcgcacgctc gcgcagaagc tcggcgagtc cctgggcgag accttcgtcg tggagaaccg
      961 ccccggcgcg aacggcggca tcggctcgga gctcgtcgcc aaggccgcgc ccgacggcta
     1021 cacgctgctc gccacggcga gcggcccgat cgtcgtgaac ccggtgctct acgccaaggt
     1081 gccgtacgac ccgctgcgcg acttcgcgcc ggtcgcccag gccaccgtgt accagtacgt
     1141 gctggtgacg ctcgccagct cgccgttcaa gagcatcgcc gacatcgtcg ccgcggcgcg
     1201 cgcgaagccc ggcgccctca ccttcggctc gaccgggatc ggcggcggca accacctcgc
     1261 gggcgagctg ctcgcgcttt cgaccggcac gcagctcacg cacgtgccct acaagggcag
     1321 cgccgcggcg ctcgccgacc tgctcggcgg acagctctcc ttcatgttcg acaccgtcat
     1381 cacctcggtg ccgcagatcc gcgccggcaa gctgcgcgcc ttcgccgtgt cgagcgcgaa
     1441 gcgcgcgtcc tcgctgcccg acgtgccgac gatgcaggaa gccgggatcg cgggcttcga
     1501 catctcgcaa tggcagggcg tgctcgcgcc cgccggcacg aaccgcgcga tcgtcgacaa
     1561 gctcaatgcc gggatccgca aggcgctcgc cgcgcccgac gtgcgcgagc gcctcgtgac
     1621 ccagggcggc aatgagatcg tcaccggctc gcccgaggat ttcgccgcgc tcatccggag
     1681 cgacctgcgc cagtacgcga agctcatccg cgacgcgaag atccccgcgc aatgaggatc
     1741 ctggtccttc ccggcgacgg catcggaccg gagatcaccg ccgccaccat cgccgtcctc
     1801 gaccgcgcca acgcgctgtt cgatctcggc ctcgagtggc acctcgagga gatcggcttc
     1861 cgcacgctga agaaggaagg cacgacgctg ccgcagcgcg tgctcgacat ggcgcgcaag
     1921 gtcgacggcg tgatcctcgg gccgctctcg acctacgagt acccgccgcg cgagcgcggc
     1981 ggcctcaacc cgtcgggcga gatgcgcgtg aagctcgacc tctacgccaa catccggccg
     2041 gcgaaatcgc gcctcggcgt cgggctcacc ggcaagccgg tcgacctcgt catctaccgc
     2101 gagaacaccg agggcttcta cgccgaccgc aacatgcacc tcggcgtggg cgagttcatg
     2161 ccgaccgagg acatggcgct ctcggtgcgc cgcgtgacgg cgaagtgctg cagccgcatc
     2221 gcgcggcgcg cgttcgaggc ggcgatgctc aggcgcaaga aagtgacggc ggtgcacaag
     2281 gcgaacgtct tcaagatctc cgacggcctg ttcctgcgcg aggtgcgcaa ggtcgcgcgc
     2341 gagttccccg aggtccagct cgaggaggtc atcgtcgacg cgatggcggc gctgctgctg
     2401 cgcgatccac agcgcttcga cgtgatcgtc gccgagaaca tgtacggcga cattctctcc
     2461 gacgaggcgt ccgagctctc gggcggcctc ggcctgggcg gctcgatcaa cgccggcgac
     2521 gacatctgcg tcgcgcaggc gcagcacggc tccgcgcccg acatcgcggg caaggaccgc
     2581 gcgaacccga cctcgctgat cctgtcggcg gcgatgctgc tcgactggat cggccggcgc
     2641 tcggggacgg aggcgctcgg cgccgcggcg cagtacatcg aggccgcggt cgacaaggtg
     2701 ctgcgcgacc ccgcgacgcg cacggcggat ctcggcggga cgctcggcac gcaggcgttc
     2761 gccaaggcgg tcgccgagac gctcggctag agcggccgct cgcgcgcacg cgccgcatgc
     2821 gcgggcggcg ccaggtgcac ccggttgcgg ccggcccgct tcgcctcgta gagcgcctcg
     2881 tcggcgcggc gcagcagatc gtcggggctg gtgtcgccgt ccgcgatggc gatcccgatg
     2941 ctggccgtca cgcgggcgcg cccggcgccg acgtcgatcc cggccggaag cgaatccacg
     3001 atcttgcacg cgaccgcccc ggcgtcatcg gcgcggttca tcccgtcgag gagaaccacg
     3061 aactcgtcgc cgccgaggcg cgccaccgtg tcgctctcgc gcacgcagct cgagaggtgc
     3121 gccgcgaact gcttcaggag cgcgtcgccg gcggcgtgcc cgagctcgtc gttgaccgcc
     3181 ttgaacccgt cgagatcgac gtagagcagc gccacgggat ggccgctgcg gcggctgcgc
     3241 gcgagcgcct gcgccagccg gtcgtcgagc aggttgcggt tcggcagccc ggtgagcgca
     3301 tcgtagcggg cggcgcgctc gagctcggcg cggctgtcga gcagcgcctg ctccgcgcgc
     3361 ttgcgctcgg tgatgtcggc gttcgtgccg accatgcgca acgcgcgtcc agcggcgtcg
     3421 cgcgcggtga cctcgccgtg gctctggatc cacagccatt gccccgccgg cgtgcgcacg
     3481 cggtgctcgg cgaagaagtg gcgcctcgct cccttcagga cctcgtgcag cgcggcccgc
     3541 atgcgcggga agtcctcggg atgcacgctc tgcgcaagct cggcgaaggt cgtgcgcagc
     3601 ggcaccgcgg cgccgccgag gagcgacgac cagcgctcgc tgaggaacac ggccccggag
     3661 gcgatgtccc agtcgaacag cgcgagctgc gagccttcca tcgccaggct gaggcggcgc
     3721 tggctctcga gcaaagcgcg ctccgccgcg ccgcgctcgc gcaccatcgc ctcgaagtcc
     3781 gccgcgagct gtccgacctc gtcgctgcgg cggacgagga cctgcggcac atcgcccgat
     3841 ccggcgcgga tccgccggat cacatcgtgc aggaccgtga gcgggccgag caggcgccgc
     3901 attcccagcc acagccccgg cgcgagcagc gcgacgaaga ccagcgcggt gagcagaacc
     3961 cgctgctgcg cttcgaccac cggcgcgtag gcctcctcga cgggcagcgc ggccaccagc
     4021 acccacggaa ccgtctgcag ctgcttggag ctgaagatgg cgtggagccc gcggctggtc
     4081 acgttctcct ccgacccttc cacgcccccg gtggcgcggt cgaactgcgg cgacacgccg
     4141 ggcgcgggtc cctgcgtcat gatgcggtcg cggtcccgcg acatcacgat ggtgcgatcg
     4201 cgggtgaaca gcgcgaacga cccggtgcgc ccgacgctgg ccgcgccgat gcctccgagg
     4261 aaattcggct tgaacaggtt gagcgtgccc gacagcaccc ccgcgacctc gccgcggcgt
     4321 ccgaagatcg gcgccgcgaa cacgatctgg gggtcgccgg tgagccggct gcgcagcggc
     4381 tcggagatca gcggcttgcc gttccgtacg acctgcctga accacgaccg gtcccccagg
     4441 ttcagcctcc gggcggagag cgcatccgga taggtggcca ggacctgccc ggacggcagg
     4501 atcgcggcga cggcatcgaa ctggctctcg aggtcgggct gattgtcgag atccgcgcgg
     4561 acgagtcgcg cgctcgacaa gtgctccgcc ggaagcacgc gggcgacgct cgccagcgac
     4621 ttcacccgga tgtcgatctt ctcgtcgatg tcctgcgcca ccatcgagac cagcgtgaac
     4681 tgctgcgcct gcagcagccg ctgcatctgc gccttggcga agtacgcttg cgcgagagag
     4741 acgagcgcga ccacgccgac cagcgcaagc gcgacggcga ccgtgatccg ggtcttgagg
     4801 ctcttggacc gcatgctgtc ggcattgcaa gccgcgggcc agtccgcgcg cccaccgcat
     4861 cgcgttcgtg acatccgcac gccggcccgc acggagccgt ttcgtccatc gccgagcgcg
     4921 gtttgatgca gatcaaatga tccttgtggc gcacggcgac cgcgggcgcc gcgcacccgg
     4981 acgtttcgga tcagggcgcc ggttcggcgc gtagcaaatc tagggctcaa tcatcgtggg
     5041 agggtctgac ctctcgcgac cgtgtcgacc atgaacaccg atgggacccc cttgcggcac
     5101 ttcgtgccgg cggccgcgct tgcgctcgcg cttttcgtcc gctggctgct cgatccgtgg
     5161 ctgcacgacg cctgggcgat gccgctcgtg ctgggcgcgg tcgccgcggc ggtgtggtac
     5221 ggcggctggg tccaggccgc gatggtcgcc ctcgccggct atctcttcag cgacttcatg
     5281 ttcatgacgc cgcgcggatc gctgtcgatc acgagcgtcg cgggcctgct cggcttcgcc
     5341 ctctattccg cgtccagcgc cctcatcatc gcgctcggcc accgcgcgcg gcgcgagcgg
     5401 cgcgaggcgc gggcgtcggc gcagctgctc gggcgcacgc tgtcgggcgc gcgcgtcgcc
     5461 gcctgggaat gggacgtgac gagcgacgtc ctgcggctga gcgaccgcac gccggacatc
     5521 ttcggcgccg gacgcgagtt caggccctcg cgccggctgc tcgcgtacat ccatcccgac
     5581 gatcgccaga cgcacgtgcg cgccgtgcgc gccgcgctgt gcggcggcga caagatcgat
     5641 tacggcttcc gcgtcgtccg ccccgacggc cgggtcgtgt ggttcgaatc gcgcggagac
     5701 gtgtcgcgcg acgcgagcgg ccgcgcgatc cggatacgag gcatcgtgat cgacgtgacc
     5761 gagcggtcgc tcgcgcatcg cgcgctgcgc gagaccgagc agcggctgca gatcgcgcag
     5821 gacctggccc cgcacggcta cgacgtgctg gagagcgtgc gcgccgaagg acgcatcgtc
     5881 gatttccgcg tcgcctacat caacgccacg ggcgcggcgc tcgccaagcg cgacccggag
     5941 gacctggtcg gcacgcgcgt tctcgacgtc tttcccgccg tggcctccac cgtgctcttc
     6001 gagcggctgc acgaaacctg cgagaccggc cgcgggtcgg acttcgaggt gcactacccg
     6061 gacgggaacg gcggcacctg gttccgctat ctcctcgcga aagtcgacag ctcggtggcg
     6121 acctccttca tcgacatcac ggcgaacaag catctcgagg aggagctgcg ccgacgcgcc
     6181 gacgcgctca aggaggcgga tcggcgcaag aacgagttcc tcgcagtgct cgcgcacgag
     6241 ctgcgcaatc cgctcgcgcc ggtctcgacc agcctgcaga tcctgaagcg catcggcggg
     6301 gactcgcgca ccgcgcgcac gctcgacgtc atggagcggc agctcgcgca gatcgttcac
     6361 ctgatcgacg acctgctcga catcagccgc atcgagcagg gcaagacgac gctgcaccgg
     6421 tccgtggtgc agctgcagga agcgctcgcc cagggcatcg acaccgccga gccgcatatc
     6481 cgcgccaagt cgcacgagct cacggtcgac atgacgccgg tgccgctgca cgtgcacgcc
     6541 gacccgatac gcatcgcgca ggtcgtcgcc aacctgctca acaacgccgc caagtacacg
     6601 cactcgcacg gccgcatccg gctggcgctg cgctccgagg cgggatacgc ggtgatcatc
     6661 gtggccgaca acggcatcgg catcccgcac gacaagctga agtcgatctt cgacctgttc
     6721 gtccaggtcg atcgcgcctc cgacaacgcg ggcggagggc tcggcctcgg gctcacgctc
     6781 gcgaagaacc tgatcgagat ccacggcggc acgatcacgg cgacgagcga ggggctcggc
     6841 aagggcagca ccttcgtcgt gcggctgccg ctcgtggtcg agcccgcgct cgagcggcgg
     6901 ccgcggcagg acgcgcgcgc cgccgccgcg caccccgcgc gccgcatcct cgtcgtcgac
     6961 gacaaccagg atgccgcgga gacgctcgcg cagctcctgc ggctcagcgg cgacgaggtg
     7021 cgcaccaccg acaacggcga ggacgcgctc gccatcggcc cggagttccg cccgcacgtc
     7081 gcgatcctcg acctgggcat gcccgacatc gacggctacg agctcgcgca gcgcgtccgg
     7141 cgccagcgat ggggcagcgg cgtcctgctc gtcgccctga gcggctgggg ccaggaggag
     7201 gacaaggtgc gctcgcgcgc ggcgggcttc gaccatcacc tcaccaagcc ggtctcgccg
     7261 ggcgatctcg agcaggtgct cgagcactac gccgcgatct gaggttccgg ggaattcgcg
     7321 cgcgccgccg tggtcgattc ccaacatgga aaccgtctga ggagcctcgc atgacgacac
     7381 acaccctgca ccacggcggc gagagcctgc cgcgctggat cgtcgtcgga ttcgtttccg
     7441 gcgccgtgtc cgttctcgtc ttccaccagg gcgcggccgc gctgctgcac gcgctcgagc
     7501 tgacgccgcg cgcgccgtac tcgatgacgc cgaccccgcc ctggggcgtg ccccaggtat
     7561 ggtcgctcgc gttctggggc ggcgtgtggg gcgtggtgtt cgccgccctg ctcgcgcgtc
     7621 tcgacggcgc ctggctggtc gtcgtctcga cggtgctcgg cgcggtgctg ccgacgctgg
     7681 tcgcatggtt cgtggtcgcg ccgctcaagg ggcagcccgc cgccgcgggc tgggcgccgg
     7741 cggccatgat gatcggcccg atcgtgaacg ccgcgtgggg cctggggacg gggctcggcc
     7801 tcgcgctcgc cggacgcacg cacgcgcgga tcgaggctga tcgaagccct agtcgaaact
     7861 gagcccgatc ccgatcaggt tccagccgcc gttctgcacg ctgcgctgga tgccgagctt
     7921 cgagccgtgc gagtggtgcc ggtactcgac gaacaggcgc cgattgatct cgtaggcgag
     7981 cgaggcgtcg aacagccagt gcgttccgtt gacgttggtc gtccggtcga tccaggcgaa
     8041 gccgatgccc gggcgccact tcccgagcgt ccacacgtgg tcgaagcccg ccatccagtg
     8101 cggcttgggc tccatatcgt cggcattcag ccacgcgccc gcgcgcaagc cccactcgcc
     8161 gccgtagcgc ccggcgagca gcgtcgcgcc tttggcgtgc acgaaatcct ccgagagcgt
     8221 gagccggaag ctcgtcggcg tcgcctcgtt ccgcagcgca tgcgcggggc cgggccacgc
     8281 gagcgcgcaa gcgaaaagca gaagcgcgcg acccctgatc acctcgaccg ccttgccgct
     8341 ctagccgcgg cgcttcttgc gcgtcgtcgc gtaccgcctg ccgagggtgc tgccgggccg
     8401 cgatgacgtc gccgggtcgc tcgcgggaaa gctctcctcg atcgactcgt ccaacatccg
     8461 ctcgtcgtgg ttgggctgtc cgggggccgg cggctccgcg ccccccgtcg tcttcgcctt
     8521 cgtctgtttc cgctttgcca tgttcgcctc ttccgacaac ctttggaacg cccccgcgga
     8581 cggcggttcg gcgccgagcc gatcggatga atttccgaga agacatgcgg tcggacgaag
     8641 gagcattggc gggggcgata gtctttttgg actgcggaag cgagggatcg acttgagcga
     8701 agacatgtac cgcgtcggga tcagcggctc ctacggcggg atgaacctgg gcgacgaggc
     8761 gatcctgcaa tcgatcgtcg cgcagctgcg ccgcgacctg cccgtcgaga tcacggtgtt
     8821 ctcgcgcaac gccgaggaca cccggaagcg ccacaaggtc gagcgcgcgg tgccggtgcg
     8881 caagctctcg cgcgccgagg tgacgcccga gatcgagcgc ctcgacctgc tgctgctcgg
     8941 cggcggcggc attctctacg atcgcgatgc ggagacgtat ttgagagaag tggctcttgc
     9001 cggggagctc ggcgttccag tgcttctcta cgcaatcagc gccggaccgc tggtcacgcc
     9061 cgcag
//
LOCUS       DATDMW010000334        10631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1843283, whole genome shotgun sequence.
ACCESSION   DATDMW010000334 DATDMW010000000
VERSION     DATDMW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10631
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843283"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VI319_13960"
     CDS             complement(<1..574)
                     /locus_tag="VI319_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6864998.1"
                     /translation="MKRYFAAVAMMLAVSAFAAGPDEIVDSLVSVKLDGGAEQTGVLT
                     MIKGDQPQALVLLMPGDPGVTRAEVADGKISSIRMRGHPFYRTRQLLVRPGLAAVLVD
                     CRSDSGDTCKENYVLSKARTDDVAKMLAAVKQQLPTVKRVWVAGHSFGGHSAASMARY
                     GEGVIDGAISASPVLAKNRTYTGLIGWDYSA"
     gene            complement(609..1424)
                     /locus_tag="VI319_13965"
     CDS             complement(609..1424)
                     /locus_tag="VI319_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6864999.1"
                     /translation="MSAATQPLLALKNVEVIYDHVILVLKGVSFSVEEGQVVALLGAN
                     GAGKTTTLKAVSNLLRSERGEVTKGSIELRGRRVDGLDPAELVKMGVVQVMEGRHCFA
                     HLTVEENLLTGAYTRGLGRGALKDDLERVYAYFPRLKARRSSLAGYTSGGEQQMTAVG
                     RALMARPAMILLDEPSMGLAPQIVEEIFGIVKSLNEKEKVSFLLAEQNTMVALRYADY
                     GYILENGRLVMNGPAQELAANEDVKEFYLGISSSGRRSFRDVKHYRRRKRWLA"
     gene            complement(1495..2826)
                     /locus_tag="VI319_13970"
     CDS             complement(1495..2826)
                     /locus_tag="VI319_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865000.1"
                     /translation="MFRHFGRPVAALLGAAILAAAPWARAQSEQFIPMNGYWVGPYAA
                     GGSGVFGGYIDYINMVNAKGGINGVKLTYEKCETEYNNARGVECYERSKKKGPTGASL
                     VMPLSTGITYSLIDKGTEDHVPIVSIGYGRTDAADGRVFPWVFPLITTYWSQATAMLK
                     FIGMKEGGGTEKLKGKKIVLVYHDSAYGKEPIPVLESQAKRFGYELHTIAVAHPGNEQ
                     QAQWLQVRQIRPDWVILWGWGVMNPTALKAAARIGFPRNKIVAVWWSGAEEDAIPAGD
                     AAKGYYAAAFNVSGSNYPVVKEIEKTVYAKGKGEMEDRSRIGSIYYNRGVVFGIVATE
                     AIRTAQAKYGKGKPMTGDQIRWGLEHLNIDEKRLKELGATNFMQPLKVSCSDHEGGGA
                     VRFLQWDGKKWNTVTDWIAADRDVVRPMIEESAAKYAKEKGITPRDCSKEG"
     gene            complement(2919..4025)
                     /locus_tag="VI319_13975"
     CDS             complement(2919..4025)
                     /locus_tag="VI319_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6865001.1"
                     /translation="MFYREAGQFKSTYAADQQLFPIRQDRIAMALLLALAFVGVPLAS
                     ITPGIEGVIDFNYWYNGVIIPFLIFSLAALGLNILTGYAGQLSLGTGAFMAVGAFAAY
                     NLMLRVPRMPILAAFVLAGVIAAAVGLVFGLPSLRIKGFYLAVATLAAQFFVVWALTK
                     FGWFSNDSSSGVIAAQKIVIAGYAFDTPVKRYLLVLAIVAVMALAAKNLARSAIGRAW
                     MAVRDMDVAAEVIGIPMMKAKLLAFAISSFYCGVAGALFAFAYLGNVEPQAFDLDLSF
                     RILFMIIIGGVGSVLGSFLGAAFIVVLPILLDIAATGFERAAGIAVPAGLVSNLTLIL
                     FGALIVFFLAVEPLGLARLWQVAKEKLRLWPFPH"
     gene            complement(4030..4923)
                     /locus_tag="VI319_13980"
     CDS             complement(4030..4923)
                     /locus_tag="VI319_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007246480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6865002.1"
                     /translation="MQFFLEVLIGGLLAGVMYSLVALGFVLIYKASGVFNFAQGSMVF
                     FAALTVVGFAEMGVPFWAAVALALAAMAALAFFTERVVLRPLVNQPQIALFMATIGLS
                     FLVEGTAQLVWGAQVHALDLGVADEPIEWILDKAGMSISKFDLMASGIAAALVAVLAV
                     FFNKTRTGRALRAVADDHQAALAVGVSLQRIWVIVWMVAGFVALVTGLLWGARNGVQF
                     SLTFLALKALPVLILGGFESIAGAIVGGLIVGASEKLAEVYVGPYVGGGIESWFPYVL
                     ALLFLLVRPEGLFGEKIIDRV"
     gene            complement(4923..5693)
                     /locus_tag="VI319_13985"
     CDS             complement(4923..5693)
                     /locus_tag="VI319_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6865003.1"
                     /translation="MSATVLAVDGISLAFGGVKALQDVSFDVHQSEIRAIIGPNGAGK
                     SSMLNVINGVYRPQAGTISYKGRTRRRFEPHEAARLGIARTFQNIALFRGMTVLDNIM
                     TGRNLKMRSGVLAQALYWGRARREELEQRAQVERIIDFLEIQHIRRTPVGRLPYGLQK
                     RVELGRALAAEPELLLLDEPMAGMNVEEKQDMCRFILDVNDQFGTTIVLIEHDMSVVM
                     DLSHRVVVLDYGRKIADGTPDEVRSNKDVIDAYLGVAH"
     gene            complement(5690..7654)
                     /locus_tag="VI319_13990"
     CDS             complement(5690..7654)
                     /locus_tag="VI319_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6865004.1"
                     /translation="MAEPALDTFPRLLLHHAAVRPARPAIREKDLGIWQTWTWAQSLE
                     HVRAIACGLAAQGFRRGDHLAVIGANRPRLYWSMMAAQALGGVPVPLYEDAIASETQF
                     VIDDAGVRFAVAEDQEQVDKLLEVLGECPRLERIYYDDPRGLRHYEQPQLASLERLEA
                     LGREAEGRDPGLFEREVAKGRAEDVATILYTSGTTGHPKGVILTHAAMIATGRAYTEF
                     EKLTADEEMLAYLPLAWVGQNLFSYSQSTVAGFCVSCPESAETVMTDLREIGPTYYFA
                     PPRVLEALLTQVTIRMEDASRPKRALYRYFMDVARRVGGRITDGRNVDGGVGFGDRLL
                     YALGDLLVYGPLRNVLGMSRVRVAYTAGEAIGPDLFVFYRSIGINLKQLYGQTETTVY
                     VCVQPDGAVKPDTVGPPLPGVELRVAESGEVLVRSPGLFREYHRDPGATAEAKDAQGF
                     FHTGDAGFLDADGHLKIIDRAKDVGRLADGTLFAPKYIENKLKFFPYIKEAVCFGHER
                     EMACAFVNIDMEAIGNWAEKRNLPYSGYVDLASQPAVIELIRDCIERVNRDLAADAKL
                     ANSQIRRFIVLHKELDADDGELTRTRKVRRRHIAEKYATLVDALYSGADHVFVETQMR
                     FEDGRTGTVSADLRIADAKLYSAAAARPAA"
     gene            complement(7679..8749)
                     /locus_tag="VI319_13995"
     CDS             complement(7679..8749)
                     /locus_tag="VI319_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEY6865005.1"
                     /translation="MRLRSSRFLLALLMPVMPVHGYLAGNNYYTLVVGFVVIALLDFA
                     FGEDRRNAAPEEAGTLQESRLHSAILYLCAAADVALIAWGASVVGTLPSLQALGLMLS
                     VGFVTGAQGITVAHELGHSRYDAERVLARVLLTAVAYGHFHVEHNRGHHVRVATPEDP
                     ASARAGESFYAFYPRAVARGWLHAWWLERERLERRGLPFWHWRNQMLWFTAVPPIAML
                     ALGLAWGPRAALFFAVQAWMAFTLLEAVNYMQHYGLSRAPLPTGAYEKVKRAHAWNAS
                     EAVSAVFLIHLARHSDHHENPWRRYPALQHHEDSPQLPTGYPGMLLLALVPPLWFSVM
                     NPRVPRVPAAFPTATDLTRDLP"
     gene            complement(8767..>10631)
                     /locus_tag="VI319_14000"
     CDS             complement(8767..>10631)
                     /locus_tag="VI319_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY6865006.1"
                     /translation="ARLARERQQLAESKRMLGTDAPTRLDALIAEREAALAPGSRELI
                     ESWPETVRSYSGEEQVVRVREREIRTRLAHESLSGSSIPKVALPKFEDHGELLAWRMR
                     ENLPGMFPFTGGVFHFKRENEDPTRMFAGEGDAFRTNRRFHLLSKDLPAKRLSTAFDS
                     VTLYGYDPDLRPDIYGKVGNSGVSIATLDDMKALYAGFDLCDPATSVSMTINGPAPTL
                     LAMFFNTAISQQLEKLGREPTDAEFQEIKARTVSTVRGTVQADILKEDQGQNTCIFST
                     EFSLKVMGVIQEWFVAHDVRNFYSASISGYHIAEAGANPISQLAFTLANGFTFVEAYL
                     ARGMKIDDFAPNLSFFFSYGMDPEYAVLGRVARRIWAVAMRDKYGGNERSQKLKFHSQ
                     TSGRSLHAQEIAFNDIRTTLQALISTYDNTNSLHTNAYDEAITTPTEDSVRRAMAIQL
                     VINKEWGLAKNENPNQGSFVIDALTDLVEEAVLREFEAISERGGVLGAMETGYQRGKI
                     QEESLLYEHRKHDGSYPIVGVNTFRKPGENAVPQRLELIRSTEEEKQSQLQRLADFHE
                     RHAKEAGPILERLRQAVIRDENVFAVLMDAVRCCSLGQITHALFEVGGQYRRNM"
BASE COUNT         1694 a         3658 c         3574 g         1705 t
ORIGIN      
        1 ccgccgagta gtcccagccg atgagcccgg tatacgtcct gttcttcgca aggaccggag
       61 acgcggaaat ggcgccgtcg atgacgccct cgccgtagcg ggccatcgac gcggccgagt
      121 gcccgccgaa gctgtgcccc gcgacccaga cccttttcac cgtcggcagc tgctgcttca
      181 cggccgccag cattttggcg acatcgtcgg tgcgcgcctt cgacagcaca tagttctcct
      241 tgcacgtgtc ccccgagtcg ctgcggcagt cgaccagcac cgcagcaagg cccggccgta
      301 cgagcagttg ccgagtccga tagaacggat ggccgcgcat cctgatgctc gagatcttgc
      361 cgtcggcgac ctcggcgcgt gtcacgcccg gatcccctgg catcagcagc acgagcgcct
      421 ggggctgatc gcccttgatc atggtcagca cgcccgtctg ctcggcgccg ccgtcgagct
      481 tcaccgagac cagcgagtcg acgatttcgt ccggcccggc cgcgaaggcc gagaccgcga
      541 gcatcatggc taccgcagcg aaatatcgtt tcatgcacct tctcctatgg tggatgcaat
      601 cgcggacctc aagcgagcca gcgtttccgg cggcggtagt gcttgacgtc acggaagctc
      661 ctgcgaccgc tcgaggagat cccgaggtag aactccttca cgtcctcgtt cgcggcgagc
      721 tcctgcgcgg gcccgttcat gacgaggcgc ccgttctcca ggatgtaccc gtaatcggcg
      781 tagcgcagcg cgaccatcgt gttctgctcg gcgagcagga agctcacctt ctccttctcg
      841 ttcagactct tcacgatccc gaagatttcc tcgacgatct gcggcgcgag ccccatcgag
      901 ggctcgtcga gcaggatcat cgccgggcgc gccatcagcg cccggccgac ggcggtcatc
      961 tgctgctcgc cgcccgaggt atagccggcg aggctcgagc gccgggcttt cagcctcggg
     1021 aagtaagcgt acacccgttc cagatcgtcc ttcagggcgc cccggcccag gcctcgcgta
     1081 tacgccccgg tcagcaggtt ctcctcgacc gtcaggtgcg cgaagcagtg ccggccctcc
     1141 atcacctgca ccacccccat cttcacgagc tccgcgggat ccaggccgtc cacgcgccgg
     1201 ccgcgcagct cgatcgagcc cttggtcacc tcgccgcgtt ccgagcgcag caggttcgag
     1261 accgccttca gcgtcgtggt ctttcccgcc ccgttcgcgc cgagcagcgc gaccacctgt
     1321 ccttcctcca ccgagaacga gacgcccttc agcacgagga tcacgtggtc gtagatcacc
     1381 tcgacgttct tcagcgcgag cagcggttgc gttgcggccg acatgcggtc ccgaaataaa
     1441 gaagggccgg gctttcgccc gacccttcgc ctgcccctcc tcctggatcc ttctctagcc
     1501 ttccttcgag cagtcgcgcg gggtgatgcc cttctccttg gcgtacttcg ccgcggactc
     1561 ctcgatcatg ggccgcacga cgtcgcggtc ggcggcgatc cagtcggtga cggtgttcca
     1621 cttcttgccg tcccactgga ggaagcgcac ggcgccgccg ccctcgtggt cgctgcacga
     1681 aaccttgagc ggctgcatga agttggtcgc acccagctcc ttcaggcgct tctcgtcgat
     1741 gttgaggtgc tcgaggcccc agcggatctg gtcgccggtc atcggcttgc ccttgccgta
     1801 cttcgcctgg gcggtgcgga tcgcctcggt ggcgacgata ccgaacacga cgccgcggtt
     1861 gtagtagatc gagccgatgc gcgatcggtc ctccatctcg cccttgccct tggcgtagac
     1921 ggtcttctcg atctccttca ccacggggta gttgctgccc gagacgttga aggcggcggc
     1981 gtagtagccc ttggcggcgt cgcccgcggg gatcgcgtcc tcctcggcgc cgctccacca
     2041 gaccgcgacg atcttgttgc ggggaaagcc gatgcgcgcg gcggccttga gcgccgtcgg
     2101 gttcatcacg ccccagcccc acaggatcac ccagtcggga cgaatctggc gcacctgcag
     2161 ccactgcgcc tgctgctcgt tgcccgggtg cgcgaccgcg atagtgtgca gctcgtagcc
     2221 gaagcgcttc gcctgagact ccagcaccgg gatcggctcc ttgccgtagg ccgagtcgtg
     2281 gtacacgagc acgatcttct tgcccttcag cttctcggtg ccgccgcctt ccttcatgcc
     2341 gatgaacttc agcatggcgg tcgcctggct ccagtacgtc gtgatcagcg ggaacaccca
     2401 ggggaacacg cggccgtcgg cggcgtcggt gcgcccgtag ccgatcgaca cgatcggaac
     2461 gtggtcttcc gtgcccttgt cgatcagcga gtaggtgatg cccgtcgaga gcggcatcac
     2521 gagcgacgcg cccgtcgggc ccttcttctt cgagcgctcg tagcactcca cgccgcgcgc
     2581 gttgttgtac tcggtctcgc acttctcgta ggtgagcttg acgccgttga tgccgccctt
     2641 ggcgttcacc atgttgatgt agtcgatgta gcccccgaat acgccggagc cgccggccgc
     2701 gtacgggccg acccagtagc cgttcatggg gatgaattgc tcgctctggg cgcgcgccca
     2761 cggcgccgct gccaggatcg cagcgcccag cagcgccgcg accggtcttc cgaagtgcct
     2821 gaacatcgcc gcctcctcga ttgggaatgc cagctctttg tggaccaccg ggactaccgc
     2881 gtgcctacta taggactgcg cccgtccctc cgcaactgct aatgcggaaa cggccagaga
     2941 cgcagcttct ccttggccac ctgccagagt cgtgccagcc cgagcggctc gaccgcgagg
     3001 aagaacacga tcagcgcgcc gaacaggatc aaggtgaggt tcgagacgag cccggccggc
     3061 acggcgatgc ccgccgcgcg ctcgaacccg gtcgccgcga tgtcgagcag gatcggcagg
     3121 accacgatga acgccgcgcc caggaacgag ccgagcacgc tgccgacgcc gccgatgatg
     3181 atcatgaaca ggatgcggaa cgagaggtcg aggtcgaacg cctgcggctc gacgttgccg
     3241 aggtacgcga acgcgaacag cgcgcctgcg acgccgcagt agaacgagct gatcgcgaac
     3301 gcgagcagct tcgccttcat catcggaatg ccgatcacct cggccgcgac atccatgtcg
     3361 cgcaccgcca tccaggcccg gccgatggcg ctgcgcgcca ggttcttcgc cgcgagcgcc
     3421 atcaccgcga cgatcgcgag caccaggaga tagcgcttca ccggcgtgtc gaaggcgtag
     3481 cccgcgatca cgatcttctg cgcggcgatc acgcccgagc tcgagtcgtt ggagaaccag
     3541 ccgaacttgg tgagcgccca cacgacgaag aactgcgcgg ccagcgtcgc gaccgcgagg
     3601 tagaagccct tgatgcgcag gctcggcagc ccgaacacga ggccgaccgc ggccgcgatg
     3661 acgccggcga ggacgaacgc cgcgaggatc ggcatcctcg gcacgcgcag catcaggttg
     3721 taggcggcga acgcgccgac cgccatgaac gcgcccgtcc cgagcgagag ctgcccggca
     3781 tagccggtga ggatgttcag cccgagcgcc gcgagcgaga agatcaggaa cgggatgatg
     3841 acgccgttgt accagtagtt gaagtcgatc acgccctcga tcccgggcgt gatcgaggcg
     3901 agcggcacgc cgacgaaggc gagcgcgagc agcagcgcca tcgcgatccg gtcctggcgt
     3961 atcgggaaca gctgctgatc cgccgcgtac gtggacttga actggccggc ttcgcgatag
     4021 aacacggcgc tagacccggt cgatgatctt ctcgccgaac agcccttcgg gccgcacgag
     4081 aagaaacagc agcgccagca cgtacggaaa ccacgactcg atgccgccgc cgacgtacgg
     4141 gccgacatag acctcggcga gcttctccga ggcgccgacg atcaggccgc cgacgatcgc
     4201 gccggcgatc gactcgaagc cgcccaggat cagcaccggc agcgccttga gtgcgaggaa
     4261 ggtcaggctg aactgcacgc cgttgcgcgc gccccagagg agtcccgtga cgagcgcgac
     4321 gaagcccgcc accatccaga cgatcaccca gatgcgctgg agcgagacgc cgaccgcgag
     4381 cgcggcctgg tggtcgtcgg cgaccgcgcg cagcgcgcgc cccgtgcgtg tcttgttgaa
     4441 gaaaacggcg agcacggcga ccagcgcggc ggcgatgccc gaggccatca ggtcgaactt
     4501 gctgatgctc atgccggcct tgtcgagaat ccactcgatc ggctcgtcgg cgacgcccag
     4561 gtcgagcgcg tgcacctgcg cgccccacac gagctgcgcc gtgccctcga ccaggaacga
     4621 caggccgatc gttgccatga agagcgcgat ctgcggctgg ttcaccaggg ggcgcagcac
     4681 cacccgctcg gtgaagaacg cgagcgccgc catcgccgcg agcgcgagcg cgaccgcggc
     4741 ccagaacggc acgcccatct cggcgaaccc gaccaccgtc agcgccgcga agaacaccat
     4801 cgagccctgc gcgaagttga acacgcccga ggccttgtag atcagcacga agccgagcgc
     4861 caccagcgag tacatcaccc ccgcgagcag gccgccgatc aggacttcga ggaagaactg
     4921 catcagtgag cgactcccag ataggcgtcg atgacgtcct tgttgctcct cacctcgtcg
     4981 ggcgtgccgt cggcgatctt gcggccgtag tcgagcacga ccacgcggtg cgagaggtcc
     5041 atcaccacgc tcatgtcgtg ctcgatcagg acgatggtcg tcccgaactg gtcgttcacg
     5101 tcgaggatga agcggcacat gtcctgcttc tcctcgacgt tcatgccggc catcggctcg
     5161 tcgaggagca gcagctcggg ctcggcggcg agcgcgcggc cgagctcgac gcgcttctgc
     5221 aggccgtacg gcagccggcc gacgggcgtg cggcggatgt gctggatctc gaggaagtcg
     5281 atgattcgct cgacctgcgc gcgctgctcg agctcctcgc gccgcgcccg gccccagtag
     5341 agcgcctgcg cgagcacgcc cgagcgcatc ttgaggttgc gcccggtcat gatgttgtcg
     5401 agcaccgtca tgccgcggaa cagcgcgatg ttctggaagg tgcgcgcgat gccgagccgc
     5461 gcggcctcgt gcggctcgaa gcggcgccgc gtccggccct tgtagctgat cgtcccggcc
     5521 tgcggccggt agacgccgtt gatcacgttc agcatcgagc tcttgccggc gccgttcggc
     5581 ccgatgatgg cgcggatctc gctctggtgg acgtcgaagg agacgtcctg caaggccttt
     5641 acgccgccga aggcgagcga gatgccgtcg acagcgagga cagtcgcgct caagccgccg
     5701 gcctcgcggc ggcggcgctg tagagcttcg cgtcggcgat ccgcaggtcg gccgacacgg
     5761 tgccggtgcg gccgtcctcg aagcgcatct gcgtctcgac gaacacgtgg tcggcgccgg
     5821 agtagagcgc gtcgaccagc gtcgcgtact tctcggcgat gtggcgccgg cgcaccttgc
     5881 gcgtgcgcgt gagctcgccg tcgtcggcgt cgagctcctt gtgcagcacg atgaagcggc
     5941 ggatctgcga gttggcgagc ttcgcgtcgg cggcgagatc gcggttcacg cgctcgatgc
     6001 agtcgcggat cagctcgatc accgccggct gcgacgcgag atcgacgtag cccgagtacg
     6061 ggaggttgcg cttctcggcc cagttgccga tcgcctccat gtcgatgttc acgaacgcgc
     6121 aggccatctc gcgctcgtgc ccgaagcaca ccgcctcctt gatgtaggga aagaacttga
     6181 gcttgttctc gatgtacttc ggcgcgaaca gcgtgccgtc ggcgagccgc ccgacgtcct
     6241 tcgcgcggtc gatgatcttg aggtggccgt cggcgtcgag gaagccggcg tcgccggtat
     6301 ggaagaagcc ctgcgcgtcc ttcgcctccg cggtcgcgcc gggatcgcgg tgatactcgc
     6361 ggaacaggcc gggcgagcgc accagcacct cgcccgattc ggcgacgcgc agctcgaccc
     6421 ccggcagcgg cgggccgacg gtgtcgggct tgaccgcgcc gtcgggctgc acgcagacgt
     6481 acaccgtggt ctcggtctgc ccgtagagct gcttcaggtt gatgccgatc gagcggtaga
     6541 agacgaacag gtcgggcccg atcgcctcgc cggcggtgta ggcgacgcgc acccggctca
     6601 tgccgagcac gttccgcagc ggcccgtaga cgagcaggtc gccgagcgcg tagagcagac
     6661 ggtcgccgaa gccgacgccg ccgtcgacgt tgcggccgtc ggtgatgcgg ccgccgacgc
     6721 gccgcgccac gtccatgaaa tagcgataga gcgcgcgctt cggccggctc gcgtcctcca
     6781 tgcggatcgt cacctgcgtg agcagcgcct cgagcacgcg cggcggggcg aagtagtagg
     6841 tcggcccgat ctcgcgcagg tcggtcatca cggtctcggc cgactccggg cacgagacgc
     6901 agaacccggc gaccgtcgac tggctgtacg agaagaggtt ctggccgacc caggcgagcg
     6961 ggaggtaggc gagcatctcc tcgtcggccg tgagcttctc gaactcggtg taggcgcggc
     7021 cggtcgcgat catcgcggcg tgcgtgagaa tgacgccctt ggggtgtccc gtcgtgcccg
     7081 acgtatacag gatcgtggcc acgtcctcgg cgcgtccttt cgcaacctcg cgctcgaaca
     7141 gcccgggatc acggccctcg gcttcgcgcc cgagcgcctc cagccgctcg aggctcgcga
     7201 gctgcggctg ctcatagtgg cgcaggccgc gcgggtcgtc gtagtagatg cgctcgaggc
     7261 gcgggcactc cccgagcacc tcgagcagct tgtcgacctg ctcctggtcc tcggccacgg
     7321 cgaagcgcac gccggcgtcg tcgatcacga actgcgtctc ggaggcgatc gcgtcctcgt
     7381 acagcggcac cggcacgccg cccagcgcct gcgcggccat catcgaccag tagaggcgcg
     7441 ggcggttcgc gccgatcacg gcgagatggt cgccgcggcg gaacccctgc gccgcgagcc
     7501 cgcacgcgat cgcgcgcacg tgctcgagcg actgcgccca ggtccacgtc tgccagatgc
     7561 cgagatcctt ctcgcggatc gcgggccggg ccggccggac cgccgcgtgg tgcagcagca
     7621 gacgcgggaa cgtgtcgagc gctggctccg ccatcggctt ctcctctccg agggcggatt
     7681 atggcaaatc gcgggtcagg tcggttgcgg tcggaaacgc cgccggtacc cgcggcaccc
     7741 gcgggttcat caccgagaac cagagcggcg gcacgagcgc gaggagcagc atgccgggat
     7801 agcccgtcgg cagctgcgga ctgtcctcgt gatgctggag cgccgggtag cggcgccacg
     7861 gattctcgtg gtggtcggaa tggcgcgcga ggtggatgag gaacacggcg ctcaccgcct
     7921 cggacgcgtt ccaggcgtgc gcccgcttca ccttctcgta ggctcccgtg ggcagcggcg
     7981 cgcgcgacag cccgtagtgc tgcatgtagt tgacggcctc gagcagggtg aacgccatcc
     8041 acgcctgcac cgcgaagaac agcgccgccc gcggccccca ggcgaggccg agcgcgagca
     8101 tcgcaatcgg cggcacggcg gtgaaccaca gcatctggtt gcgccaatgc cagaatggca
     8161 ggccgcggcg ctcgagccgc tcgcgctcga gccaccacgc gtgcagccag ccgcgcgcga
     8221 cggcgcgcgg gtaaaacgcg tagaagctct cgccggcgcg cgccgacgcc gggtcctcgg
     8281 gcgtcgccac gcgcacgtgg tgcccgcggt tgtgctcgac gtggaagtgg ccgtacgcga
     8341 ccgcggtgag cagcacgcgg gcgagcacgc gctcggcgtc gtagcggcta tggccgagct
     8401 cgtgcgcgac cgtgatcccc tgcgcgccgg tgacgaagcc caccgagagc atcaggccga
     8461 gcgcctggag cgaaggcagc gtaccgacca ccgacgcgcc ccaggcgatc agcgcgacat
     8521 cggcggcggc gcaaaggtag aggatcgcgg aatgcaggcg cgactcctgc agcgtcccgg
     8581 cctcttcggg cgcggcgttg cgccggtcct cgccgaaggc gaaatcgagc agcgcgatga
     8641 cgacgaagcc gacgacgagc gtgtagtaat tgttgcccgc gagatacccg tgcacgggca
     8701 tcacgggcat cagcagcgcg agcaggaagc ggctggagcg gaggcgcacg gcgccatgtt
     8761 acgccgctac atgttccgcc ggtactggcc tccgacctcg aacagcgcgt gcgtgatctg
     8821 tccgagcgag cagcagcgca cggcgtccat gagcacggcg aagacgttct cgtcgcggat
     8881 caccgcctgg cgcaggcgct cgagtatggg cccggcttcc ttcgcgtgcc gctcgtggaa
     8941 gtcggcgagg cgctggagct gcgactgctt ctcttcttcc gtggaccgga tcagctcgag
     9001 gcgctgcggg accgcgttct cgccgggttt tcggaaagta ttgacgccga cgatcggata
     9061 cgagccgtcg tgcttgcggt gctcgtagag cagcgactcc tcctggatct tgccgcgctg
     9121 gtagccggtc tccatcgcgc cgagcacgcc gccgcgctcc gagatcgcct cgaactcgcg
     9181 cagcaccgcc tcctcgacca ggtcggtgag cgcgtcgatg acgaagctcc cctggttcgg
     9241 gttctcgttc ttcgcgaggc cccattcctt gttgatgacg agctggatcg ccatcgcgcg
     9301 gcgcaccgag tcctcggtgg gcgtggtgat cgcctcgtcg taggcgttgg tgtgcagcga
     9361 gttggtgttg tcgtaggtcg agatcagcgc ctgcagcgtg gtgcggatgt cgttgaacgc
     9421 gatctcctgc gcgtgcagcg agcgccccga ggtctgcgag tggaatttga gcttctggct
     9481 gcgctcgttg ccgccgtact tgtcgcgcat cgccaccgcc cagatgcggc gcgcgacgcg
     9541 cccgagcacg gcgtactccg gatccatgcc gtagctgaag aagaacgaga ggttcggcgc
     9601 gaagtcgtcg atcttcatgc cgcgcgcgag ataggcctcg acgaaggtga agccgttcgc
     9661 gagcgtgaag gcgagctgcg agatcgggtt cgcgccggcc tcggcgatgt ggtagcccga
     9721 gatcgacgcc gaatagaaat tgcgcacgtc gtgcgcgacg aaccactcct ggatgacgcc
     9781 catcaccttg aggctgaact cggtcgagaa gatgcacgtg ttctggccct ggtcctcctt
     9841 caggatgtcg gcctgcaccg tgccgcgcac ggtcgagacg gttctcgcct tgatctcctg
     9901 aaactccgcg tcggtgggct cgcgtccgag cttttcgagc tgctgcgaga tcgcggtgtt
     9961 gaagaacatc gcgagcagcg tcggcgccgg tccgttgatc gtcatcgaca ccgaggtcgc
    10021 cgggtcgcac aggtcgaagc ccgcgtacag cgccttcatg tcgtccagcg tcgcgatcga
    10081 cacgcccgag ttgccgactt tgccgtagat gtccggacgg agatcgggat cgtagccgta
    10141 gagcgtcacc gaatcgaagg ccgtggacag gcgcttggcc ggcaggtcct tcgacagcag
    10201 gtgaaagcgc cggttggtgc ggaacgcgtc gccctcgccc gcgaacatgc gcgtcgggtc
    10261 ttcgttctcg cgcttgaagt ggaacacgcc gccggtgaac gggaacatgc ccggcaggtt
    10321 ctcgcgcatg cgccaggcga gcagctcgcc gtggtcctcg aatttcggca gcgcgacctt
    10381 cgggatgctc gagcccgaga gcgactcgtg cgcgagccgg gtgcggatct cgcgctcgcg
    10441 cacgcgcacg acctgctcct cgcccgagta cgagcggacc gtttccggcc acgactcgat
    10501 cagctcgcgc gatccgggcg cgagcgcggc ctcgcgctcg gcgatcaggg cgtcgagccg
    10561 cgtcggcgcg tccgtcccca gcatgcgctt ggactccgcg agctgctggc gctcgcgcgc
    10621 gagccgcgcc t
//
LOCUS       DATDMW010000335         8463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2089739, whole genome shotgun sequence.
ACCESSION   DATDMW010000335 DATDMW010000000
VERSION     DATDMW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089739"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VI319_14005"
     CDS             <1..737
                     /locus_tag="VI319_14005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6865007.1"
                     /translation="VAKAAPDGYTLGFNNQGSVAMAPFSLSSMPYDPNRDLALITTVV
                     KVPEVIVLHPSVPANTLAELLAYARTHPGKVNYGSAGAGGITHLAGELLKSEAKIDIV
                     HVPYKGAAPAVNDLLGGQVQMGFFDVPVVLPHIKAGKLKALAVTSAKRAPSLPDVRTT
                     AEEGYPNVISDNWYGLVAPAKTPAPVLRRVHDASVAALKTPELLEQFAKVSGVAAPST
                     PQEYARFLGDEQKRWGGIIKAIGFKE"
     gene            complement(774..1574)
                     /locus_tag="VI319_14010"
     CDS             complement(774..1574)
                     /locus_tag="VI319_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6865008.1"
                     /translation="MSGRLQGRVAIVSGAGSVGPGWGNGRAIAVRFAEEGAKVFAVDR
                     EPERLAETIERVRQAGGEICTHHCDVTRSAEVAAMIDACVEAWGRVDILVNGVGGSAA
                     GGPVEMTEEVWDTQVDYNLKSVFLGCKHAIPVMERQGGGAIVNIASTSGTRWTGSAQV
                     AYAATKAGVIQLSRVVAVQYAKKGIRVNTVVPGQLHTPMVEARLAKQRAGGDVKAILE
                     QRAKRIPLAIEGDGRDTANAALYLASDEARFVTGTEIVVDGGMTLRCD"
     gene            complement(1571..2458)
                     /locus_tag="VI319_14015"
     CDS             complement(1571..2458)
                     /locus_tag="VI319_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6865009.1"
                     /translation="MSDPKPSFHPSPGKPKLRLPALACDAHVHVFGPSRVFPFAPERP
                     FTPADAPKEKLFAMHARMGIERCVIVQSTCHGYDNRVVADAIAAKRGAYCGVALVPPT
                     IADADLRRLDALGFRGVRFNFVTHLGVGTAPAEAIALSARLAPLGWHLQVHFAPELID
                     ELAPFLLRSAVPVVIDHMGRVDASLGVSQPAFRKLMQLMRDPRFRVKVSGSERLSREG
                     PPWKDARPFARALVSEFGDRAFWGTDWPHPNLAFVPDDGALVDLLAEIAPTQAERQAL
                     LVDNPERFYGFPVKLKEAA"
     gene            2537..3268
                     /locus_tag="VI319_14020"
     CDS             2537..3268
                     /locus_tag="VI319_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865010.1"
                     /translation="MLPPMPNKNEPATFLASVVFLKIHEFARRPVQEQTRLRAQLEAV
                     VAVAAAGIAPADRVVLDASDGIALVVLGAPRAALATAERALAASAAGLPLAVGINHGA
                     VALSGAKKRDGMTGDGIAVAASAAEFSSPGRVLASRAFRDAIADESPGAEASLVPAGT
                     FTDAGLRTHELYTPDAAAPGRRRRRLNALAAASVVLFVGAGIGMRASSDPRQPRFLDD
                     TIAKVRQSTRDSQAFLRGLVQKAKF"
     gene            3272..4417
                     /locus_tag="VI319_14025"
     CDS             3272..4417
                     /locus_tag="VI319_14025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6865011.1"
                     /translation="MATRKQARIGRYKIAAELGRGAMGVVYKAKDPVLDRTVALKTIG
                     MGEDASGRSEYQARFFLEAKAAGKLNHPNIITIHDYGEEGELAWMAMELLEGTELRAR
                     MQEGQVPLAEAIDIAQQVAEGLGYAHEHGVVHRDIKPSNIMLMKRGKVKIMDFGIARM
                     RASDLKTSTGVVLGTPRYMSPEQVAGEPVDHRSDIFSLGTVLYEMLTRTSLFAGNDTP
                     QILHNVQAFQPVAPSRLNPEVPAMLDFVVARALKKEPDVRYQDAYEMAADLRACLAEL
                     RGREPEPEGERSATKTIKLDAEPTRVVAPAAGAIGSDTRLPLSARFDCTAALDRLVTP
                     GRRDAARLRRAPRPVGLARRLLRDPGQRLLFTVALAAAVAGGMIAFG"
     gene            4593..4772
                     /locus_tag="VI319_14030"
     CDS             4593..4772
                     /locus_tag="VI319_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865012.1"
                     /translation="MAGESKTRFCTTGSHWTTAEFRKIGPIRWICAACYERRKIELRN
                     LPANRQLAAGVRPKQ"
     gene            4955..5176
                     /locus_tag="VI319_14035"
     CDS             4955..5176
                     /locus_tag="VI319_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865013.1"
                     /translation="MDKRETLLHARWNAAMERYRTAQEQVSRDYLLRPDGVPTRETMQ
                     KANAARNEVASVRRLVECNPPRSRRARWD"
     gene            5214..5474
                     /locus_tag="VI319_14040"
     CDS             5214..5474
                     /locus_tag="VI319_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865014.1"
                     /translation="MRTYLLTPVRFDAPEWEYSLSREPVQVRAETESRARELATLRFV
                     IGAAAGKSPWSDASLVQARQVDSLQPGTPLLDEHAMARRGHR"
     gene            5572..6825
                     /locus_tag="VI319_14045"
     CDS             5572..6825
                     /locus_tag="VI319_14045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY6865015.1"
                     /translation="MSVSELRNAAGDPAGSAASSCATSRRAAAAVMRAWSIEDLRRIA
                     RNRLPRAVFDFYDGGAEDEATLRANRAAFERVRLAPRTLVDVTRADTSTAILGGRSAL
                     PIAIGPTGAPGFGWPLGDVGIARAAAAAGIPYTLSTMATASIERIAREAPGRLWFQAY
                     VFKTREITMELIERARRFGYEALMITVDVPVGGKRERDFRNDFAVPFRFTPRNVLDFA
                     THPGWVLSVLRHGMPVLENVAGFTPEARSSAEIASSIGRFWDPAFDWDRMKEIRDRWA
                     GKLLVKGVLRGDDAEKLAALGVDAIVVSNHGGRQLDGASAALDALPHVVRAVNRKASV
                     MVDGGVRRGVDVVKALALGADAVMLGRATLMGVCAAGEAGARRAIEILRDELVRAMQL
                     SGVTAVGQIGPDLIAPPLAGATSCP"
     gene            6816..7181
                     /locus_tag="VI319_14050"
     CDS             6816..7181
                     /locus_tag="VI319_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6865016.1"
                     /translation="MPVRKILVVDDSATNRAFLSEMLGRHGYQCTTAQSGAEAIEKAR
                     TGQPDLILMDVVMPDMDGYQATRALVKDEQTKHIPIILCTSKNQATDELWGLRQGAKA
                     YVIKPVKEKDLLSKIAAVG"
     gene            complement(7178..7534)
                     /locus_tag="VI319_14055"
     CDS             complement(7178..7534)
                     /locus_tag="VI319_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865017.1"
                     /translation="MTQATFHCDCGADPSIRSIRLSVVNLNPPYGAAQVRAASQSPWI
                     RLERIAKPVDVAAPTPYVEVELLDQTRSCTLACIGWTGSDGTLHPCPEIREMTLKQGD
                     TNFRPQPATPRPPRQA"
     gene            7953..>8463
                     /locus_tag="VI319_14060"
     CDS             7953..>8463
                     /locus_tag="VI319_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865018.1"
                     /translation="MLAAIQSLDLESVKVRIMDPKLGEGWSREYADSIEGAYKTYLSM
                     LAKHPEDAEDIMLSKDVDEFWHTHILQTIKYHEDCEAVFGKYLHHQPHIGELTDADHD
                     KRNALADKTRRLYEAEFGGGGSSVAWSGVPVNARGAALSSVAIGPARAALSSAAVRAD
                     GAALSSAAIR"
BASE COUNT         1306 a         2974 c         2955 g         1228 t
ORIGIN      
        1 cggtcgcgaa agccgcgccc gacggctaca cgctcggctt caacaaccag ggctcggtcg
       61 cgatggcgcc gttctcgctc tcgagcatgc cctacgatcc gaacagggat ctcgcgctca
      121 ttaccaccgt cgtcaaggtc ccggaagtga tcgtgctcca tccctcggtg ccggcgaaca
      181 cgctcgccga gctcctcgcc tacgcgagga cccaccccgg caaggtcaac tacggctccg
      241 ccggcgcggg cggcatcacg cacctcgcgg gcgagctgct gaagtcggaa gcgaagatcg
      301 acatcgtcca cgtcccctac aaaggcgccg cgcccgcggt gaacgacctg ctcggcgggc
      361 aggtgcagat gggcttcttc gacgtgcccg tcgtcctgcc gcacatcaag gccgggaagc
      421 tgaaggcgct cgccgtgacg agcgcgaagc gcgcgccgtc gcttcccgac gtgcgcacca
      481 cggcggaaga gggctacccg aacgtgatca gcgacaactg gtacggcctg gtcgcgccgg
      541 cgaagacgcc ggccccggtg ctcaggcgcg tgcacgacgc gtcggtcgcc gcgctcaaaa
      601 cacccgagct gctcgagcag ttcgcgaaag tcagcggcgt cgccgcgccc tcgacgccgc
      661 aggagtacgc caggttcctc ggcgatgagc agaagcgctg gggcgggatc atcaaggcga
      721 tcgggttcaa ggaatagatg tcattctgag gccgcgaagc tcaggatgac agctcagtcg
      781 cagcgcagcg tcatcccccc gtccaccacg atctcggtcc cggtcacgaa ccgcgcctcg
      841 tccgacgcga gatagagcgc cgcgttcgcc gtgtcgcgcc cgtcgccttc gatcgccagc
      901 ggaatccgct tcgcgcgctg ctcgaggatg gccttcacgt cgccgcccgc gcgctgcttc
      961 gccaggcgcg cttccaccat cggcgtgtgc agctgtcccg gcaccacggt gttcacgcgg
     1021 atgcccttct tcgcgtactg cacggcgacg acgcgcgaga gctggatgac gccggccttg
     1081 gtcgcggcgt aggcgacctg cgccgagccg gtccagcgcg tgccggaggt cgaggcgatg
     1141 ttgacgatgg cgccgccgcc ctggcgctcc atgaccggga tcgcgtgctt gcagccgagg
     1201 aagacgctct tgaggttgta gtcgacctgc gtgtcccaca cttcctcggt catctcgacc
     1261 ggaccgccgg cggccgagcc gccgacgccg ttgacgagga tatcgacgcg gccccaggcc
     1321 tcgacgcagg cgtcgatcat cgcggcgact tcggcgctcc gcgtgacgtc gcagtggtgc
     1381 gtgcagatct ctccgccggc ctggcgcacg cgctcgatcg tctcggccag gcgctcgggc
     1441 tcgcgatcca ccgcgaacac cttggcgcct tcctccgcga agcgtaccgc gatggcgcgg
     1501 ccgttgcccc agcctgggcc gacgctgccc gcgcccgaga cgatcgcgac cctgccctga
     1561 agtcgtccgc tcacgccgct tccttcagct tcacgggaaa gccgtagaag cgctcgggat
     1621 tgtcgacgag cagcgcctgg cgctcggcct gggtcggcgc gatctcggcg agcagatcga
     1681 cgagcgcgcc gtcgtcgggc acgaacgcga ggttcgggtg cggccagtcg gtgccccaga
     1741 acgcgcggtc gccgaactcg gagacgaggg cgcgcgcgaa cgggcgcgcg tccttccacg
     1801 gcggtccttc acgcgacagg cgctcgctgc cgctcacctt gacgcggaag cgcggatccc
     1861 gcatcagctg catgagcttg cggaacgcgg gctgcgagac gccgagcgag gcgtcgacgc
     1921 gccccatgtg gtcgatcacg accggcaccg ccgagcgcag caggaagggc gcgagctcgt
     1981 cgatcagctc gggcgcgaaa tgcacctgca ggtgccagcc gagcggcgcg agccgcgccg
     2041 agagcgcgat cgcctcggcg ggcgccgtgc cgacgcccag gtgcgtgacg aagttgaagc
     2101 gcacgccgcg aaagccgagc gcgtcgagcc gccgcagatc ggcgtcggcg atcgtcggcg
     2161 gcacgagcgc gacgccgcag tacgcgccgc gcttcgcggc gatcgcgtcg gcgaccacgc
     2221 ggttgtcgta gccgtggcag gtcgattgca cgatcacgca gcgctcgatg cccatcctcg
     2281 cgtgcatggc gaagagcttc tccttcggcg cgtcggccgg cgtgaacggc cgctcgggcg
     2341 cgaacgggaa cacgcggctc ggtccgaaca cgtgcacgtg cgcgtcgcac gcgagcgcgg
     2401 gcaggcgcag cttgggtttg ccgggcgacg gatggaagga aggcttcggg tcggacatgg
     2461 gggacgagcg ggcaatatac cgcaggcggc gcgaggcggt cgcggtgctt gcttcccaaa
     2521 attcgggaaa cccgtcatgc tgccgcccat gcccaataag aacgaacccg cgacgtttct
     2581 cgcgagcgtc gtcttcctca agatccacga gttcgcgcgc cgcccggtcc aggagcagac
     2641 gcgcctgcgc gcgcagctcg aggccgtggt cgcggtcgcc gccgcgggca tcgcgccggc
     2701 ggaccgcgtg gtcctcgacg cctcggacgg catcgcgctg gtcgtgctgg gcgcgccgcg
     2761 cgcggcgctc gcgacggccg agcgcgcgct cgcagcgtcc gccgccggcc tgccgctcgc
     2821 ggtcggcatc aatcacggcg cggtcgcgct ctcgggcgcg aagaagcgcg acggcatgac
     2881 cggcgacggc atcgcggtcg ccgcgagcgc ggctgagttc tcgtcgccgg ggcgcgtgct
     2941 cgcctcgcgc gcgttccggg acgcgatcgc cgacgaatcg cccggcgccg aggcgtcgct
     3001 cgtgcccgcc ggcacgttca ccgacgcggg cctgcgcacg cacgagctgt atacgccgga
     3061 cgcggccgcg cccgggcgcc ggcggcggcg attgaacgcg ctcgccgcgg cgagcgtggt
     3121 gctgttcgtc ggcgccggca tcggcatgcg cgcgtcgtcg gatccgcgcc agccgcgctt
     3181 cctcgacgac accatcgcga aagtgcgcca gtcgactcgc gacagccagg ccttcctgcg
     3241 cggcctggtg cagaaagcga agttctagcg catggcgacc cggaagcaag cgcgcatcgg
     3301 ccgctacaag atcgcggccg agctcggacg cggcgcgatg ggcgtcgtgt acaaggcgaa
     3361 ggacccggtg ctcgaccgca ccgtcgcgct caagaccatc ggcatgggcg aggacgcctc
     3421 cgggcgctcg gagtaccagg cgcgcttctt cctcgaggcg aaggcggccg gcaagctcaa
     3481 ccatccgaac atcatcacca tccacgacta cggcgaggag ggcgagctcg cgtggatggc
     3541 gatggagctg ctcgagggca ccgagctgcg cgcgcgcatg caggagggcc aggtcccgct
     3601 cgccgaggcg atcgacatcg cgcagcaggt cgccgagggc ctcggctacg cgcacgagca
     3661 cggcgtcgtc caccgcgaca tcaagccgag caacatcatg ctgatgaagc gcggcaaggt
     3721 gaagatcatg gacttcggca tcgcgcgcat gcgcgcctcc gacctgaaga cctcgaccgg
     3781 cgtcgtgctc ggcacgccgc gctacatgtc gcccgagcag gtcgcgggcg agccggtcga
     3841 ccaccgctcc gacatcttct cgctcggcac cgtgctgtac gagatgctga cgcgtacctc
     3901 gctgttcgcg ggcaacgaca cgccgcagat cctgcacaac gtgcaggcat tccagcccgt
     3961 cgcgccgagc cggctcaatc ccgaggtgcc ggccatgctc gacttcgtcg tcgcgcgcgc
     4021 gctcaagaag gagcccgacg tgcgctacca ggacgcctac gagatggcgg ccgacctgcg
     4081 cgcctgcctc gcggagctgc gcgggcgcga gcccgagccg gagggcgagc gcagcgccac
     4141 caagacgatc aagctcgacg ccgagccgac gcgagtggtc gcgcccgcgg cgggggcgat
     4201 cggctcggac acgcggctgc cgctgtcggc gcgcttcgac tgcaccgccg ccctcgaccg
     4261 gctcgtcacc cccgggcggc gcgacgccgc ccgcctgcgg cgggcgccgc gtcccgtggg
     4321 gctcgcccgc cggctcctcc gggacccggg acagcgcctg ctgtttacgg tggccctggc
     4381 cgcggcggtc gcgggcggca tgatcgcctt cggctgagtc gccgggccgg tttcccgcac
     4441 tgccgcagag acttatcgac cgggttcctt cggccccttg ccattcggcc cgggacaccg
     4501 cacacttacg actcttcagc gccgcggcaa cgcggtattc caggtgtcgg gtccccgcga
     4561 gggggtgccc ttttttggtc ttctgagacg acatggccgg agaatcgaaa actcgctttt
     4621 gcaccacggg cagccactgg accacggcgg agttccgcaa gatcgggccg atccgatgga
     4681 tttgcgccgc gtgctatgag cgacgcaaga tcgagctgcg caatctgccg gcgaaccggc
     4741 agctcgccgc cggcgtacgc ccgaagcagt aacggggacg cgcaagcgtc ttttgtcgcg
     4801 ctgcggcgcg acttctcctt tcgacgcaga agtttttttg ttggccgcgc ggcggaaccc
     4861 gcccgcgaga gccgcctcca agtctatccg cggggcgtga tgacgccgtc atgcgtcccc
     4921 atccgagagt cgagacttag aaagaggagg tcctatggac aagcgagaga ccctgttgca
     4981 cgcgcgctgg aacgcggcca tggagcggta tcggaccgca caggagcagg tgtcccggga
     5041 ttacctcctg cgtcccgacg gcgttccgac ccgggaaacc atgcagaaag cgaacgccgc
     5101 gaggaacgaa gtcgcgagcg tgcgccggct cgtggagtgc aatccgccgc gctcgcgccg
     5161 cgcccgctgg gactgagcgg ccggtcggcg ccgccggcgc ggcgctcggc gccatgcgga
     5221 cctacctgct gaccccggtc cggttcgacg cgccggagtg ggaatacagc ctgtcgcgcg
     5281 agccggttca ggtgcgggcc gagaccgagt cgcgggcgcg cgagctcgcg acgctgcgct
     5341 tcgtgatcgg cgccgccgcg ggcaagagcc cgtggagcga cgcgagcctg gtgcaggcac
     5401 ggcaggtcga tagcctccag cccgggacgc ccctgctcga cgagcatgcg atggcgcggc
     5461 gcgggcatcg ctgacgccgc cgtcggcgcg cgcctccccg tgcgccgggc ccggtcgcgg
     5521 tagaatgccg ggttgatccg gccgtcaagg ccgcagggag attccagaac gatgagcgtg
     5581 agcgaactgc ggaacgcggc gggcgacccc gcgggctccg cggcgtcctc gtgcgcgacg
     5641 tcgcggcgcg ccgccgccgc cgtcatgcgc gcctggtcga tcgaagactt gaggcgcatc
     5701 gcccgcaacc ggctgccgcg agcggtgttc gacttctacg acggcggcgc cgaggacgaa
     5761 gccaccctgc gcgcgaaccg cgccgcgttc gagcgcgtgc gcctcgcccc gaggacgctc
     5821 gtcgacgtga cccgcgccga cacctccacc gccatcctcg gcggccgctc cgcgctgccg
     5881 attgcgatcg gccccacggg ggctcccggc ttcggctggc cgctcggcga cgtcggcatc
     5941 gcgcgcgccg ctgccgcggc cggcatcccc tatacgctct cgaccatggc caccgcgtcg
     6001 atcgagcgca tcgcgcgcga ggctccgggg cgcctctggt tccaggccta cgtgttcaag
     6061 acgcgcgaga tcaccatgga gctgatcgag cgcgcgcgcc gcttcggcta cgaggcgctc
     6121 atgatcacgg tcgacgtgcc ggtgggcggc aagcgcgagc gcgacttccg caacgacttc
     6181 gccgtgccgt tccggttcac gccgcgcaac gtgctcgact tcgcgacgca cccgggctgg
     6241 gtgctctcgg tgctgcgcca cgggatgccg gtgctcgaga acgtcgccgg cttcacgccg
     6301 gaggcgagga gctcggccga gatcgcctcc tcgatcggac gcttctggga cccggcgttc
     6361 gactgggacc ggatgaagga gatccgtgac cgctgggccg gcaagctgct cgtgaaaggc
     6421 gtgctgcgcg gcgacgacgc cgagaagctc gccgcgctcg gcgtcgacgc gatcgtggtc
     6481 tcgaaccatg gcgggcgcca gctcgacggc gcctcggccg cgctcgatgc attgccgcac
     6541 gtggtgcgcg cggtgaaccg caaggcgagc gtcatggtcg acggcggcgt gcggcgcggc
     6601 gtcgacgtgg tgaaggcgct cgcgctcggc gccgacgccg tcatgctcgg ccgcgcgacg
     6661 ctgatgggcg tgtgcgccgc gggcgaggct ggcgcgcggc gcgcgatcga gatcctgcgc
     6721 gacgagctgg tgcgcgcgat gcagctctcc ggcgtgaccg cggtcggaca gatcggcccc
     6781 gacctcatcg cgcctcccct ggcaggagcg acgtcatgcc cgtgagaaaa atcctcgtcg
     6841 tcgacgactc ggcgaccaac cgcgcgttcc tgtccgagat gctcgggcgg cacgggtatc
     6901 agtgcacgac ggctcagagc ggcgccgagg ccatcgagaa ggcgcgcacc gggcagcccg
     6961 atctcatcct gatggacgtc gtcatgcccg acatggacgg ctaccaggcg acgcgcgcgc
     7021 tggtcaagga cgagcagacc aagcacatcc cgatcatcct ctgcacgagc aagaaccagg
     7081 cgaccgacga gctctggggc ctgcgccagg gcgcgaaggc ctacgtgatc aagcccgtca
     7141 aagagaagga cctgctctcc aagatcgcgg ccgtcggcta ggcctgccgc ggcggacgcg
     7201 gcgtggccgg ctgcggccgg aaattcgtgt cgccctgctt cagcgtcatt tccctgatct
     7261 cggggcacgg atgcagcgtg ccgtccgacc ccgtccagcc gatgcacgcg agcgtgcacg
     7321 agcgcgtctg atcgagcagc tcgacctcga cgtaaggcgt cggcgccgcc acgtcgaccg
     7381 gcttggcgat ccgctcgagc cggatccagg gtgactgcga cgccgcgcgc acctgcgcgg
     7441 cgccgtaggg agggttgagg ttcaccacgc tcaggcggat cgaccggatg ctcgggtcgg
     7501 cgccgcaatc gcagtggaaa gtggcctgcg tcatcgcgaa tccctcgtct ttcttatgtt
     7561 gttcggagtc ccgggcgagg gccttcggca gcgttggccc gcgaggcgct cctcttagct
     7621 tcgcggggcc ctacattaat ccaggatttg ccctacatca actgtccaca aaaacctaca
     7681 cctctgtttc ctgacgctcg gtagattcgc cgaagggccg gcgttagcgc atcggtggca
     7741 ttccctggga cggggccgcc gggcccctcc cctaagagcc cccatggcgc ggcctttgaa
     7801 gcaagcgctt acccgatcag cctacgacag gaggtggccg taacccgaaa tctatttttc
     7861 gcttcaggca gttgaaggac ttcttagcac tacttttttt ctcaccgagt caggagagga
     7921 ctatgttgca cacaggaaag cccgtcgaag tcatgctcgc cgcgatccaa tctctggatc
     7981 tcgagagcgt caaggttcgg atcatggacc ccaagctcgg agaaggctgg agccgggagt
     8041 acgcggacag catcgagggg gcgtacaaga cgtatctgtc gatgctggcc aagcaccctg
     8101 aagatgccga ggacatcatg ctgagcaagg acgtggacga gttctggcat acccacatct
     8161 tgcagacgat caagtaccac gaggactgcg aggcggtgtt cgggaagtat ctgcatcatc
     8221 aaccgcacat cggggaattg acggacgccg accacgacaa gcggaacgcg ctcgcggaca
     8281 agacgcgcag gctgtacgag gccgagttcg gtggtggcgg ctcgagcgtg gcgtggtccg
     8341 gcgtgccggt caacgcccgc ggagctgccc tgtcttcagt ggcgatcggg ccggcccgtg
     8401 cggcgctctc gagcgccgca gtccgcgccg atggcgcggc tctctccagc gcggccattc
     8461 ggg
//
LOCUS       DATDMW010000336         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2216981, whole genome shotgun sequence.
ACCESSION   DATDMW010000336 DATDMW010000000
VERSION     DATDMW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216981"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VI319_14065"
     CDS             complement(<1..611)
                     /locus_tag="VI319_14065"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEY6865019.1"
                     /translation="MSARRKVAIAVTALAALLGIAAGCAPSRDWRTASQEPVGLAPDP
                     ETTADAVIQVYAARTVGWKSAFGVHTWIAVKPGGVHKPYTVYEVIGWRLRWSDSALAM
                     RQRAPDARWFGAEPELIAERRGPGVDALIARIDAAARAYPWADEYTLWPGPNSNTFTA
                     WVLRAVPELGVDLPPTAIGKDYMGSRLVGSAPSGRGVQLSLFGL"
     gene            complement(613..1587)
                     /locus_tag="VI319_14070"
     CDS             complement(613..1587)
                     /locus_tag="VI319_14070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865020.1"
                     /translation="MTFLGPTARWAVLAALALTLPAWAQDYPTRAIKIVVTFTPGGAS
                     DLAARVVGLKLSDALGQPVVVENRLGANGTIGTQMVAQSEPDGYTLVIGTNPNITINP
                     HMRKLAFDPMKDLVPVSMLTETSMILFVNPSVVPVKDVKEFVAYAKAHPGKVYYASAG
                     SGSLAHLAGELFKLAAGISLVHVPYKGGTLGVNDVLAGAVGSMFASVPAVAPQIHSGK
                     VRGMAVTGPKRSVFAPEIPTMVESGYPDVEALLWNGLFAPAGTPEPVLRKLREETAKI
                     LARKDVQELFVKQGAEAFPMSEKEFRAFVQREYERWGRVIKEAHIKVE"
     gene            complement(1584..2351)
                     /gene="ttcA"
                     /locus_tag="VI319_14075"
     CDS             complement(1584..2351)
                     /gene="ttcA"
                     /locus_tag="VI319_14075"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY6865021.1"
                     /translation="MKNRLLRQVGEAIADYEMIAQGDRVMVCLSGGKDSYGMLDVLLE
                     LRARAPVGFELIAVNLDQRHPGYPEDVLPKYLESRGVPYRIEVQDTYSIVKRVVPEGD
                     TMCGLCSRLRRGVLYRVAREVGATKIALGHHRDDILETFFLNLFFGGTMKAMSPKLRS
                     DDGAHVVIRPLAYVRETDLEAWAKLREFPIIPCDLCGSQETLQRKQVKQMLREWEKRH
                     PGRIESMFRALSDVIPSHLMDRKLFDFSSIAPKGAAE"
BASE COUNT          371 a          820 c          799 g          361 t
ORIGIN      
        1 agaccgaaga gcgagagctg cacgccgcgg ccgctcggcg cggacccgac gagccgcgag
       61 cccatgtagt ccttgccgat cgcggtcggc ggcagatcga cgccgagctc gggcaccgcg
      121 cgcagcaccc acgcggtgaa ggtgttcgag ttcggccccg gccacagcgt gtactcgtcg
      181 gcccagggat atgcgcgggc cgcggcgtcg atgcgcgcga tgagcgcgtc cacgcccggc
      241 ccgcgccgct cggcgatcag ctcgggctcg gcgccgaacc agcgcgcatc gggcgcgcgc
      301 tggcgcatcg cgagcgcgga gtcgctccag cgcagccgcc agccgatgac ctcgtacacc
      361 gtataaggct tgtgcacgcc gcccggcttg accgcgatcc aggtgtgcac gccgaacgcg
      421 ctcttccagc cgacggtgcg cgcggcgtag acctggatga ccgcatccgc ggtcgtctcc
      481 ggatccggcg cgagaccgac cggctcctgg ctcgcggtgc gccagtcgcg cgagggcgcg
      541 cagcccgcgg cgatgccgag cagcgcggcg agcgccgtga cggcgatggc gaccttgcgg
      601 cgagcgctca tctcactcca ccttgatgtg cgcctctttg atgacgcgtc cccagcgctc
      661 gtactcgcgc tgcacgaacg cccggaactc cttctcgctc atcggaaacg cttcggcgcc
      721 ctgcttgacg aagagctcct ggacgtcctt acgggcgagg atcttggcgg tctcctcgcg
      781 cagcttgcgc agcacgggct cgggcgtccc ggccggcgcg aacaggccgt tccacagcag
      841 cgcctcgacg tcgggatagc cggactcgac catggtcggg atctcgggcg cgaacaccga
      901 gcgcttcggc ccagtcaccg ccatcccgcg cacctttccg gagtggatct gcggcgcgac
      961 cgcgggcacc gacgcgaaca tcgagccgac cgcgccggcg agcacgtcgt tcacgccgag
     1021 ggtcccgccc ttgtacggca cgtggacgag cgagatgccc gcggcgagct tgaaaagctc
     1081 cccggcgagg tgcgcgagcg acccgctgcc ggccgaggcg tagtacacct tgccgggatg
     1141 cgccttggcg tacgcgacga actccttcac gtccttgacc ggcacgacgc tcgggttcac
     1201 gaaaaggatc atcgacgtct cggtgagcat cgacaccggc acgaggtcct tcatcggatc
     1261 gaatgcgagc ttgcgcatgt gggggttgat cgtgatgttc gggttggtgc cgatcaccag
     1321 cgtgtagccg tcgggctccg actgcgcgac catctgcgtg ccgatcgtgc cgttggctcc
     1381 gaggcggttc tcgaccacga ccggctgccc cagcgcgtcc gacagcttca gcccgacgac
     1441 gcgtgcggcg agatccgagg cgccgcccgg cgtgaaagtg accacgatct tgatggcgcg
     1501 ggtcggataa tcctgcgccc aggcgggaag cgtcagcgcg agcgcggcga gaacagccca
     1561 ccgcgcggtc ggtccaagaa acgtcattct gcggctcctt tcggcgcgat cgaggagaaa
     1621 tcaaagagct tgcggtccat caggtgagaa ggaatcacgt ccgagagcgc gcggaacatc
     1681 gactcgatgc gtcccgggtg gcgcttctcc cattcgcgca gcatctgctt cacctgcttg
     1741 cgctgcagcg tctcctgcga gccgcacagg tcgcagggga tgatcgggaa ctcgcgcagc
     1801 ttcgcccacg cctcgaggtc cgtctcgcgc acgtaagcga gggggcggat cacgacgtgc
     1861 gcgccgtcgt cgctcctgag cttcggtgac atcgccttca tcgtcccccc gaagaacagg
     1921 ttgaggaaga acgtctcgag gatgtcgtcg cggtgatgcc cgagcgcgat cttggtcgcc
     1981 ccgacctcgc gcgcgacgcg gtagagcacg ccgcggcgca gccgcgagca cagcccgcac
     2041 atcgtgtcgc cctcggggac gacgcgcttc acgatcgagt aggtgtcctg cacctcgatg
     2101 cggtaaggca cgccgcgcga ctcgaggtat ttcggcagga cgtcctcggg atatcccggg
     2161 tggcgctggt cgaggttgac cgcgatcagc tcgaacccga ccggcgcgcg cgcgcgcagc
     2221 tcgagaagca cgtcgagcat accgtagctg tccttgccgc cggaaaggca gaccatgacc
     2281 cggtcgcctt gcgcgatcat ctcgtagtcg gcgatggcct cgcccacctg gcgcagcagg
     2341 cgattcttca g
//
LOCUS       DATDMW010000337         2574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_596497, whole genome shotgun sequence.
ACCESSION   DATDMW010000337 DATDMW010000000
VERSION     DATDMW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596497"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VI319_14080"
     CDS             complement(<1..465)
                     /locus_tag="VI319_14080"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEY6865022.1"
                     /translation="MTASAVHRVRRQTWRVRVGSAPAAFAARARLSAEAEDLLPAFGR
                     AFDAWAPGEAVLRIPRLELSLKVRSLDEIAAKLDEALRRAAPVPAAHAEGPRPAAADA
                     LRVLLHYLGTGQLEWHAAHVEPAAAAAALRDTLLDALPAAVRAAPSGAIAFEA"
     gene            complement(462..>2574)
                     /locus_tag="VI319_14085"
     CDS             complement(462..>2574)
                     /locus_tag="VI319_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865023.1"
                     /translation="PVARPVLSGALATENDLAAVYGAACDAIHNALLAALGALWAPAK
                     WMLRDIAPTDPGPRWQARLTALHDAAPVRGRQYYYDFLKDLAETCNAFRDCLFGDTAV
                     CCPALDAFAKHLLLGSLDPAQRAAVGRTGFYPSPAVSDRFEQRAHARFLVRKIDALIA
                     DFAIPGPGQVRITPSAFEERPLEERAIPFYYAPGTALPIQTLWSERLTRRGATATAYS
                     YNAPNWAAPQSPAASPLAFQTGAFGFYRVEGHIGRPLADAQREIDALVAGSNLPIDVA
                     YVALAQPVKPPFPWWRNWHLYDLQNLMRNELGTQLDDTAQFGDVFLGQAKTAAQGSIV
                     TDQDNGGVPVVGTVQTKVELMKGKAGAARAKVMAETYDPASNWQDDFAGATQAAAAMH
                     LALGPVTQKQFVSPADSVIAGQPARWLNWIDILIKGQEDDAARRSQLPGYLADHPGLE
                     HYAGVVRGGTFVVVYDGANVVVADFMLPYRCCEPKAAPPKPPVLVPLPRPPVIYQPIR
                     WVTLPDKFQFTKLSDTVINQVKGDVANQVSYLSGFKDTLSMFVGAKASGPVSLPALTG
                     GSATAADPLPADPALKFKIVDIAQKQQLLDQIRVQKLDPTLDPAAQKELDVQQAATEN
                     DLAASIVDVTAHVANTGIAVTPGTDGALAIQAASSAFNNISNLDALNHVETSVMNLVN
                     TQAAGSNVGQALNTMIGLRIR"
BASE COUNT          344 a          885 c          965 g          380 t
ORIGIN      
        1 ggcctcgaac gcgatcgcgc ccgaaggcgc cgcgcgcacc gccgcgggaa gcgcatcgag
       61 cagcgtgtcg cgcagcgccg cggccgcggc cgcgggctcc acgtgcgcgg cgtgccattc
      121 gagctgcccg gtcccgaggt aatggagcag aacgcgcaac gcatccgccg cggcggggcg
      181 cggaccctcc gcgtgcgccg cgggcacggg tgccgcacgc cgaagcgcct cgtcgagctt
      241 cgcggcgatc tcgtcgagcg agcgcacttt cagcgacagc tcgaggcgcg ggatgcgcag
      301 caccgcctct cccggcgccc aggcgtcgaa cgcgcgtccg aacgcgggca gcaggtcctc
      361 ggcctcggcg ctcagccgcg cgcgcgcggc gaaggcggcc ggcgccgagc cgacgcgaac
      421 gcgccaggtc tgcctgcgca cgcgatgaac cgcggatgcc gtcaccgtat cctcagcccg
      481 atcatcgtgt tgagcgcctg ccccacgttg ctgcccgcgg cctgggtgtt gaccagattc
      541 atcacgctgg tctcgacgtg gttgagcgcg tcgaggttac tgatgttgtt gaacgcgctc
      601 gacgccgcct ggatcgccaa ggcgccgtcg gtgccgggcg tgacggcgat gccggtgttg
      661 gcgacgtgcg ccgtgacgtc gacgatgctc gcagccaggt cgttctcggt cgcggcctgc
      721 tgcacgtcga gctccttctg ggccgccggg tccagcgtcg ggtcgagctt ctgcacgcga
      781 atctgatcga gcagctgctg cttctgcgcg atgtcgacga tcttgaactt cagcgccggg
      841 tcggcgggca ggggatcggc ggccgtcgcg gagccgcccg tcagggcagg cagcgagacc
      901 gggccgctcg ccttcgcacc gacgaacatg ctgagcgtgt ccttgaatcc gctcaggtag
      961 ctcacctggt tcgccacatc gcccttcacc tggttgatca cggtgtcgct gagcttggtg
     1021 aactggaact tgtcgggcag cgtcacccag cggatcggct ggtagatcac gggcgggcgc
     1081 ggcagcggca cgagcaccgg cggcttgggc ggcgcggcct tcggctcgca gcagcggtag
     1141 ggcagcatga agtccgcgac gacgacgttc gcgccgtcgt agacgacgac gaaggtgccg
     1201 ccgcgcacca cgcccgcgta gtgctccagg ccgggatggt cggcgagata gccgggcagc
     1261 tggctgcgcc gcgcggcgtc gtcctcctga cccttgatca ggatgtcgat ccagttcagc
     1321 cagcgcgcgg gctgccccgc gatcaccgag tcggccggcg acacgaattg cttctgcgtc
     1381 accggtccga gcgcgaggtg catggcggcc gcggcctggg tggcgccggc gaagtcgtcc
     1441 tgccagttcg acgccgggtc gtaggtctcg gccatgactt tggcgcgcgc cgcgcccgcc
     1501 ttgcccttca tcagctcgac cttggtctga acggtgccga ccacggggac gccgccgttg
     1561 tcctggtcgg tgacgatgct tccctgcgcc gcggtcttcg cctggccgag gaagacgtcc
     1621 ccgaactgcg cggtgtcgtc gagctgcgtg ccgagctcgt tgcgcatcag gttctgcagg
     1681 tcgtacaggt gccagttgcg ccaccacggg aacggcggct tcaccggctg cgcgagcgcg
     1741 acgtaggcga cgtcgatcgg caggttcgac ccggcgacga gcgcgtcgat ctcgcgctgc
     1801 gcgtcggcga gcgggcggcc gatgtggccc tcgacgcggt agaagccgaa ggcgccggtc
     1861 tggaacgcga gcggcgacgc cgccggggac tgcggtgccg cccagttggg cgcgttgtac
     1921 gagtacgcgg tcgcggtcgc gccgcggcgc gtcagccgct cgctccacag cgtctggatc
     1981 ggcagcgcgg tgccgggcgc gtagtagaac gggatcgcgc gctcctcgag cggccgctcc
     2041 tcgaacgcgc tcggcgtgat gcgcacctgc ccgggtcccg ggatcgcgaa gtcggcgatc
     2101 agcgcgtcga tcttgcgcac gaggaagcgc gcgtgcgcgc gttgctcgaa gcggtcgctg
     2161 accgcgggcg agggatagaa gccggtgcgg ccgaccgcgg cgcgctgcgc cgggtcgagg
     2221 ctgccgagca gcaggtgctt ggcgaaggcg tcgagcgcgg gacagcacac cgcggtgtcg
     2281 ccgaacaggc aatcgcggaa ggcattgcag gtctcggcga ggtccttcag gaaatcgtag
     2341 tagtactgcc gcccgcgaac cggcgcggcg tcgtgcagcg ccgtgagccg cgcctgccag
     2401 cgcgggccgg gatcggtcgg cgcgatgtcg cgcagcatcc atttcgccgg cgcccagagc
     2461 gcgccgagcg cggcgagcag cgcgttgtgg atcgcgtcgc acgcggcgcc gtagacggcg
     2521 gcgaggtcgt tctcggtcgc gagcgcgccc gagagcaccg gccgcgcgac cgga
//
LOCUS       DATDMW010000338        21442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1344384, whole genome shotgun sequence.
ACCESSION   DATDMW010000338 DATDMW010000000
VERSION     DATDMW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344384"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VI319_14090"
     CDS             complement(<1..1313)
                     /locus_tag="VI319_14090"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY6865024.1"
                     /translation="MLLALLLVPVLWTDMPVVNAMRLAWLDFYQALSPRERRSAPAII
                     VAIDEKSIDRYGQWPWPRDLVAKLLDRIRAAGPAAIGVDLLFPNADRLSPEQLAPTLA
                     GYNAALAAGLAKLPSHDVQLAHAIKAAPTVLGIAGLEMATGSSVTAQFTPSFQKGGDA
                     TPFLRHYAETLRSIPGIDKSAQGHGLLSVDTEGGVVRRMPVVAAVGHTPVLGFSIETL
                     RVASGDPVFTVNSDATGVRSISVGGVDIPTQPDGHIWVHYTPHNTARYVSAADVLAGT
                     VEDSELGGMLVLVGLSALGLIDYQTTPLGERMPGVEIHAQVLENIFDGRLLSRPRWAP
                     WAEGCSFLLFGLLLAYAVPRLTPRRSGVLLAAMLATLMGAGFVAYKSAGLLVDALVPA
                     AALASLFAAMLAATLAQANAQRRLLRERLQREREAAAQIAGELDAA"
     gene            complement(1412..2380)
                     /locus_tag="VI319_14095"
     CDS             complement(1412..2380)
                     /locus_tag="VI319_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6865025.1"
                     /translation="MPAVRVKFWGTRGSLPIAFDAAALKDRLASALVAASGHDVGTIE
                     KARAWLERGPGSALSQTFGGDSPCVQLEAGAAEYVLCDLGTGARVFANRVLQRHGATS
                     PQVFHVFMSHLHWDHIMGFPFFTPAYLPGNRIVIYGCHRELEHAFRSQHAAPFFPIPF
                     ERLGARIEFVTLAPGRPYDIAGWSVTPRRQDHSGDSYGYRFERDRRSIVYSTDSEYKL
                     DNAADTQAATAFFRDADLVIFDAMYSLADSVSVKEDWGHSSNVVGVELCQLARAKHLV
                     LFHHEPANNDDKIASIWRETVRLEEITRHGAPLRVSAAYDGLEIEL"
     gene            complement(2384..2767)
                     /locus_tag="VI319_14100"
     CDS             complement(2384..2767)
                     /locus_tag="VI319_14100"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6865026.1"
                     /translation="MLISQKRYANVKVLTLSGRIDNGTSDSFKTGLGAHVAECRAGGD
                     RLVLDFSGVDYISSVGLRALMLAARQSREQGGTIVMAALQPVVREIFEISRFTMVFDC
                     FPTVREALAKISPSALAAYDARTPG"
     gene            2848..3960
                     /locus_tag="VI319_14105"
     CDS             2848..3960
                     /locus_tag="VI319_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY6865027.1"
                     /translation="MKLPSLSRLAKPCVAFFVALASAGPGLAQRLPIDSIKLPPGFRI
                     SVFTDKVPEARSMSLGPDGVLFVGTRGDRVYAVRYRGDKVTGVTTVASGLNLPNGVAF
                     RDGALYVAEIDRILRFDDIAAKLDSPPKPVVVTDRYPGDRAHGAKLVRFGPDGLLYVP
                     VGMPCNVCERNPDRYGVISRIRPDGSGYEVFARGVRNSVGFDWDPRTKELWFTDNGRD
                     MMGDDLPSDELNHAPKPGMNFGFPYCHQGDTPDPEFRRHGCAEFAPPAVKLGPHVAAL
                     GMRFYTGDAFPAEYRNNIFVAEHGSWNRSAKIGYRVARVVVEGGRAVKHEVFAHGWLQ
                     GRSAWGRPVDVQPTPDGALLVSDDEAGVIYRISYHK"
     gene            4059..6887
                     /locus_tag="VI319_14110"
     CDS             4059..6887
                     /locus_tag="VI319_14110"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY6865028.1"
                     /translation="MDDTATKVSTLPEQEICREALIEKYAKGKEQSIDDVRRRIARAL
                     AAVEPQDRRSHWERRFHEAMEAGFIPAGRISSAAGIQMQATLINCFVQPVGDSISEIV
                     DGRPGIYTALQEAAETMRRGGGVGYDFSSIRPRGAEVKGTHSRASGPVSYMRVFDRSC
                     ETVESAGARRGAQMGVLRCDHPDLEDFIHAKDKGDLSNFNISVGVTDVFMTAVERDLE
                     VELVHKAKPHPDAIAAGAYQRDDGLWVYRKLRARDLWDQVMRSTYDHAEPGILFLDRI
                     NRDNNLNYCEAIEATNPCAEQPLPPYGCCCLGSINLTRFVKEPFSENAAFDFEGFAKI
                     VEVSVRMLDNVLDVTAWPLKAQHEEAMNKRRVGLGFTGLGDALIMLRLRYDTDDARRT
                     AARISESMRDAAYRASSELARERGAFPLFNADMYLSGTSFAARLPQEIKTLIRKQGVR
                     NSHLLSIAPTGTISLAFADNASNGIEPPFSWTYTRKKRMADGTLKEFPVEDYAWRLYK
                     HLGGDAKALPPYFVTALELTAAAHKDMVAAVAPSIDTSISKTVNVPENYPYSDFQGLY
                     FAAWKSGLKGLATYRPNSVIGSVLSVEAQTRPQDFVQNDVNRRIEIKAVPEPVLASLR
                     WPGRPKLAAGNPAWTYMIEHPHGDFALFVGHVENARAHPFEAWVNGSEQPRGLGAVAK
                     TLSMDMRADDPAWLRLKLDTLAKTAGDDAFDMPFPPLGEKKRMPSACSAVAQLVRFRC
                     EQLGALPESAHGPVIDAMFSLKEPKTGTDGTMSWTIDIHNPAARDDFVLGLKEITLPD
                     GVTRPYSLWLSGEYPRALDGLCKLLSLDMRIIDPAWIGMKLRKLLDYPEPLGDFMAFV
                     PGSRKQQNWPSTVSYVARLVIHRYAMLGILDEDGMPVQQMGVLETPAPGAAPKMKGLR
                     CTECGNYAVIRKDGCDFCSACGWVGVCG"
     gene            complement(6894..7304)
                     /locus_tag="VI319_14115"
     CDS             complement(6894..7304)
                     /locus_tag="VI319_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865029.1"
                     /translation="MLLAASTAFADQGSAGRTAAKDISKKAADTGHAIKDYSVAQRDE
                     AVKQAKAALDDADARMHRLERKLDADWGKMDAAARQRARATREAMRRERNQVAEWYGG
                     LKHGSSEAWDEVKSGFAKSYDELRKSLSKARNDF"
     gene            complement(7431..8093)
                     /locus_tag="VI319_14120"
     CDS             complement(7431..8093)
                     /locus_tag="VI319_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6865030.1"
                     /translation="MTKRTVLAFLSFLAAAVALSGCADPRSHTNAVYMLVDTSGTYAR
                     EANKAQIIVNYLLGTLQPGDSLAIARVESRSFSEKDIIAKARFDTRPSQANMQKRAFK
                     DKMDGYFRGIRGTAYTDITGGLIQGAEFLNETGAGMKTILIFSDMQEELDKATVRNFA
                     INLKNIRVIAVNVTKLKTDNIDPRRYLGRIDAWQKRVEAAGASEWKVINDIEHLDGIF
                     KS"
     gene            complement(8119..9555)
                     /locus_tag="VI319_14125"
     CDS             complement(8119..9555)
                     /locus_tag="VI319_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865031.1"
                     /translation="MVDSHVWMIWPNNAVLSVITLCLIGIAFLYAGRRPMHDLIRSLG
                     HSLSGPLRLGARSLVAAANGIRERNRAVLLAHGRAEVGQRIEREFERIGAIVTRDLQG
                     YPAMQRKLLDEITRIEEDYKKCGEVPPPPPEWVEAVTAVAKVKGNGNEIVQKILEEIN
                     VSVGKMHDKTIGEYRRAYESRHKILEGFLPFWRSVDKTMTLVDKKLSGIQETAGTVDA
                     HMAKYEEINKKTDKAEHQLATSGFTQFAIATIVMFVAMGGAFVNFKLIALPMSEMVGA
                     GDYITQSLRTSEVAALVIILVEASMGLFLMESLRITHLFPPIANLNEKMRHRILYISL
                     TLLVILAGIEAALALMRDMLIADKQALLQTLASQQQTAVNEGWVGRIPTAGQMLLGFI
                     LPFALAFVAIPLESFIHSSRTVLGVAVVALLHALAFVLRVLGNLARHSSRVLITLYDV
                     VVMLPLLIEHLVKGGRVGVPVKSRSMSA"
     gene            9896..10582
                     /locus_tag="VI319_14130"
     CDS             9896..10582
                     /locus_tag="VI319_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865032.1"
                     /translation="MGWEAGLQASRYRYEEPDFAKLTGNRAGLSAAYTEADGREFGRL
                     EARYSYGSLTYQGSGTQNNVPDHIFELRAIGGADYRPVASVSLSPFAGLGYRYLYDDL
                     RGYDVVGNVAYFGYRRYSQYLYVPLGLTARLSAGRGWVLAPTLEYDVFLRGRQQSKLS
                     DAGFGAPDITNEQRHGRGYRAAFMIEAGHLAFGPWIDYWSIKDSEVSNGFLEPANHTR
                     EAGIEVRYRF"
     gene            complement(10590..11153)
                     /locus_tag="VI319_14135"
     CDS             complement(10590..11153)
                     /locus_tag="VI319_14135"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY6865033.1"
                     /translation="MPDPRKDLTEHTLSSEVVYDGRLLHVRRDKVRLPDGGVTDREYV
                     RHPGAVVIIAVLDQGELLMERQFRYPLGRDMIELPAGKIDAGEPPAETARRELLEETG
                     YTASEWRHLATMHLAIGYSNERIEIFLATGLTRKEAKLDDEEFLEVFTLPLASALQWV
                     REGKITDAKTLSALLWAEKVLEGAWRP"
     gene            complement(11168..12676)
                     /gene="nuoN"
                     /locus_tag="VI319_14140"
     CDS             complement(11168..12676)
                     /gene="nuoN"
                     /locus_tag="VI319_14140"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY6865034.1"
                     /translation="MNLIPAYPELWLLVMAPVVLIVDLFVRDQRRDVTYALALAALAG
                     CAFWTLVTGHLTGLQRTEAFNRMFVADTMGHVLKMLCYVAVGASLVYSRAYLRDRGLY
                     RGEYFALALFSTLGMMVMISANHFLSLYLGLELMSLSLYALVALNRDSAASTEAAMKY
                     FVLGALASGLLLYGMSMIYGATGTLYIGDVAKAIGANQPDRIILLFGLVFAVSGMAFK
                     LGVVPFHMWIPDVYHGAPVSVTLIIGSASKLAAFAMVVRMLVNGTLGFVEGGGRIDLL
                     SSLSQNLVFNWQYMLVILAVLSMAIGNLAAIAQSNIKRMLAYSTISHMGFVLLGLLSG
                     VVGNVFSAANAYSAALFYVIVYALMSLGAFGMILLLSRAGFEAERLDDFKGLNARSPW
                     FAFVMMLLMFSLAGIPPTVGFYAKVVVLQPVLDAGQVWLAVVAVLFSLIGAFYYIRIV
                     KLMYFDEPQDAHRIETRADMRVLLSLNGMAMLALGILPQSLMQLCLLSIKGL"
     gene            complement(12673..14172)
                     /locus_tag="VI319_14145"
     CDS             complement(12673..14172)
                     /locus_tag="VI319_14145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY6865035.1"
                     /translation="MTSSAPWLSLAIWIPIVAGCIVLATGDRNSREARWIALAGAVIG
                     LLVTLPLVAGFDPSTSAMQFVEPPRTWIARFNVQYRVGVDGISVLFVLLNSFITVLVV
                     AAAWESVQNKVAQYLAAFLIMSGLLNGTFAALDGLLFYVFFEATLIPMYLVIGVWGGP
                     NRVYAAVKFFLYTLLGSLLMLLAFLYLYMKSGGSFSILDWHNVPIGRGEHFWNDPQIL
                     LFLALFAAFSVKVPMWPVHTWLPDAHVEAPTGGSVVLAAILLKLGGYGFIRFSLPILP
                     GASHDLAGFMIVLSLVAIVYIGLVALVQTDMKKLIAYSSISHMGFVTLGTFLFNPLAL
                     EGALVQMISHGFVSAALFLCVGVLYDRMHSRNIADYGGVVNVMPRFAALMMLFAMANC
                     GLPATSGFVGEFMVIMGSMKVNFWLAFLAATTLVFGAAYTLWMYKRVVFGAVGNDHVA
                     ALRDAGMRELVVLGALAAAVLWMGVHPAPFTDIMHASVDNLLKHIATKP"
     gene            complement(14183..16177)
                     /gene="nuoL"
                     /locus_tag="VI319_14150"
     CDS             complement(14183..16177)
                     /gene="nuoL"
                     /locus_tag="VI319_14150"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY6865036.1"
                     /translation="MSLYSLCLLVPFPPLAGAIVAGLFGRRVGRAGAHWAAILGVAIA
                     FIASCVVLGRVLNGETLNQPLYTWLASGDLRLEIGFLVDRLTALMMVVVTFVSLMVHV
                     YTIGYMADDPGYQRFFAYIALFTFSMLMLVMANNFLQLFFGWEAVGLVSYLLIGFWYT
                     RPSAIYANLKAFMVNRVGDFGFLIGVALVLATFGTLDYGAVFSRAPTLAQATIEAIPG
                     WPWLLITAICIGLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVA
                     RMSPLFELSETALSFVLVIGGITTFFMALIALVQYDIKRVVAYSTLSQLGYMTVALGA
                     SAYPVAIFHLMTHAFFKALLFLGAGSVIIALHHEQDMRKMGGLWRYMPVTYATVFIGA
                     LANAAIPGFSGFFSKDSIIEAVHLSKTPGAGFAWLMVTACVFVTAFYTFRLVFYTFHG
                     KERFGDAGHEHAGHGHAPHESPPVVTIPLVLLAIPSIAAGWVVGPVLFGNYFDGSIYV
                     SPDHSGLREMAEEYHGIGAMMQHALGSLPFWLSLAGIALAWFMYILRPEVPARVRARA
                     GVLVTILEEKYGFDRFNDWFFAGGMRKLGTGLWRWGDVTFIDGLMVNGSARLVGWFAG
                     VVRWIQSGFIYDYAFSMIIGAFVLLTVWVFVVPWLQRYLV"
     gene            complement(16185..16490)
                     /gene="nuoK"
                     /locus_tag="VI319_14155"
     CDS             complement(16185..16490)
                     /gene="nuoK"
                     /locus_tag="VI319_14155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY6865037.1"
                     /translation="MLSLSDFLILGAVLFAISVVGIFLNRKNVIILLMAIELMLLSVN
                     MNFIAFSHYLGDMSGQVFVFFILTVAAAESAIGLAILVALFRNLQTINVDDLDQLRG"
     gene            complement(16538..17140)
                     /locus_tag="VI319_14160"
     CDS             complement(16538..17140)
                     /locus_tag="VI319_14160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY6865038.1"
                     /translation="MSFETFTFYVFAVILVLSALRVITARNPVHSALFLVLAFFTSAA
                     LWVLLRAEFLAITLVLVYVGAVMVLFLFVVMMLDINLDRLREGFWSYLPLGATVGVLM
                     VIEMGLVLWNRYSGLQSAVPAQPQQAVSNTKALGRLLYTDYAYPFELAAVLLLVAIVA
                     AIALTLRRRKDTKYQDPAKQVLVRRSERVRLVSMPAEKRD"
     gene            complement(17147..17638)
                     /gene="nuoI"
                     /locus_tag="VI319_14165"
     CDS             complement(17147..17638)
                     /gene="nuoI"
                     /locus_tag="VI319_14165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY6865039.1"
                     /translation="MLDKIRDFFKTFLLVELLKGMALTGRNLFARKITIQFPEEKTPM
                     SPRFRGLHALRRYPNGEERCIACKLCEAICPALAITIESDQRADGTRRTTRYDIDLTK
                     CIFCGMCEESCPVDSIVETRILEYHGEKRGDLVYTKEMLLAVGDRYEEQIAKDRAADA
                     AYR"
     gene            complement(17642..18721)
                     /gene="nuoH"
                     /locus_tag="VI319_14170"
     CDS             complement(17642..18721)
                     /gene="nuoH"
                     /locus_tag="VI319_14170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY6865040.1"
                     /translation="MMSTLTDWSNAILSTSWGPPAWLVVKTVLLILAISIPVILCVAY
                     LTLWERKFIGYMQVRIGPNRVGPKGLLQPFADVLKLLVKEIIVPAGANRFLFFAAPIL
                     TLMPAMAAWAVIPFGPETVLANINAGLLYVMAITSMGVYGIIIAGWASNSKYSFLGAM
                     RSAAQIVAYEIAMGFALVCVLLVSQSLNLSDIVAAQQRGFFADKGINLLSWNWLPLFP
                     MFLVYFISGTAETNRAPFDVAEGESEIVAGFHVEYSGMTFALFFLAEYMNMILVATLA
                     TIMFLGGWAPLWDSAFTNAIPPFVWLVAKVFVMVTMFLWFRATFPRYRYDQIMRLGWK
                     VFIPLTIIWLVVIAAWMQTPWSPWR"
     gene            complement(18718..21048)
                     /gene="nuoG"
                     /locus_tag="VI319_14175"
     CDS             complement(18718..21048)
                     /gene="nuoG"
                     /locus_tag="VI319_14175"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY6865041.1"
                     /translation="MPKVTIDGLETEVQNGATVMDAANALGIYVPHFCYHKKLTIAAN
                     CRMCLVQVEKAPKPMPACATPVTEGMKVLTRSDNARQAQNGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGKPSSRYAEEKRVVLNKNLGPLISTDMTRCIHCTRCVRFGQEIA
                     GVMELGMIGRGEHAEIISFVGRTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRR
                     KAVSPHDSLGSNLIVQVKLNKVMRVLPLENESLNECWLSDKDRFSYEGLNSDERLLRP
                     MLKENGKWREIDWQPALEHVAARLKDAKARHGAESIGALGSAHSTLEELHLLQKLVRG
                     LGSENVDFRVRQSDFSSDGKRAGVPWLGAQVADLGKLDRALVVGSFLRKDHPLVAARL
                     RGAAKRQAEINMLHCADDDLLMPVANKLVVAPQALPEALAQIVKAAAEIAQKPAPASV
                     SAVTAGAGAKAIAASLASGKNAMILLGNTATQHPQSAQLHALAHELAGLLGVNLGFLG
                     EAPNSVGGYLVNCVPGARGLNAQSMLEQPRKAYLLLNLEPELDCHDPAAALRALRAAD
                     FVVALTAYGSTVAAEYADVLLPIGPFTETSGSFVNCEGRLQSFNGAVKPLGDARPAWK
                     VLRVLANLLGVPGFDQDSSEAVRDEACPVQSIPGRLNNALRGVEISLESGGDGLRRIA
                     DVPIYFTDPIVRRATSLQKTRDALPPRAWMASATLQRLGLAAGDRVRVRQGEGQAVLE
                     AGRDDGLPLDCVRLAAGHRLTAELGPMFAAVEVERA"
     gene            complement(21053..>21442)
                     /locus_tag="VI319_14180"
     CDS             complement(21053..>21442)
                     /locus_tag="VI319_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY6865042.1"
                     /translation="GSMLGSGAVIVMDETTCMVKALERLSYFYFEESCGQCTPCREGT
                     GWLYRMVHRIEHGKGRNEDLDLLTNVADNIAGRTICALGDAAALPVKSFILHFRDEFQ
                     YHIDHRRCQVSEPGYSTAPTVPQRRAA"
BASE COUNT         3853 a         7169 c         7069 g         3351 t
ORIGIN      
        1 gccgcgtcca gctcgccggc gatctgcgcg gccgcctcgc gctcgcgctg cagccgctcg
       61 cgcagcaggc gccgctgcgc gttcgcctgc gccagcgtcg cggcgagcat cgcggcgaaa
      121 agcgaagcga gcgccgcggc cggaaccagt gcgtcgacga gaagacccgc gctcttgtag
      181 gcgacgaagc cggcgcccat gagcgtcgcg agcatcgcgg cgagcaggac gcccgagcgc
      241 cgcggtgtca ggcgcgggac ggcgtacgcg agcagcagtc cgaacaggag aaacgagcag
      301 ccttccgccc acggggccca gcgcgggcgc gaaagcagcc tgccgtcgaa gatgttctcg
      361 aggacctgcg cgtggatctc gacgcccggc atgcgctcgc cgagcggcgt cgtctggtag
      421 tcgatcagcc cgagcgcact caagccgacg aggacgagca tgccgcccag ctcggagtct
      481 tccaccgtgc cggccaggac gtcggccgcc gagacgtagc gcgccgtatt gtgcggcgtg
      541 tagtggaccc agatgtggcc gtcgggctgc gtggggatgt ccacgccgcc gacgctgatc
      601 gagcgcaccc cggtcgcgtc gctgttgacg gtgaacaccg ggtcgccgct cgccacgcgc
      661 agcgtctcga tgctgaaacc cagcaccggc gtgtggccga ccgccgcgac gacgggcatt
      721 cgccggacga cgccgccttc ggtatcgacg ctgaggaggc cgtgtccttg cgcggacttg
      781 tcgatccccg ggatgctgcg cagcgtttcg gcgtagtgcc tcaggaacgg cgtcgcgtcg
      841 cctcccttct ggaacgacgg cgtgaactgg gccgtaaccg agctgcccgt cgccatctcg
      901 agacccgcga taccgagaac ggtgggcgcc gccttgatcg cgtgcgccag ttgcacgtcg
      961 tgcgacggca gcttcgccag tccggctgcg agcgcggcgt tgtatcccgc gagcgtgggc
     1021 gccagctgct cgggcgagag gcggtccgcg ttcggaaaca gcaggtcgac gccgatcgcg
     1081 gccggcccgg cggcgcggat gcgatcgagc agcttcgcga ccagatcgcg cggccacggc
     1141 cactgcccgt atcgatcgat gctcttctcg tcgatcgcca cgatgatcgc cggcgcagag
     1201 cggcgctcgc gcggcgacag cgcctgatag aagtcgagcc aggcgagccg catggcgttc
     1261 accacgggca tgtcggtcca gagcaccggg accagcagca gggcgagcag cacgaccgag
     1321 cgcagcgcac gccacggaag cgcggaccgc ggtgcgaccg tcagccgatc ggtcgtcgtc
     1381 ccggccgctg cgctgctgcc tgtcggcgag ctcagagctc gatctccagc ccgtcgtagg
     1441 ccgccgagac ccggagcggc gcgccgtggc gcgtgatctc ctccaagcgt acggtctcgc
     1501 gccatatcga ggcgatcttg tcgtcgttgt tcgccggctc gtggtggaaa agcaccaggt
     1561 gcttcgcccg tgccagctgg cacagctcga cgccgacgac gttgctcgaa tggccccagt
     1621 cctccttcac cgagacgctg tcggccagcg agtacatcgc gtcgaagatg acgaggtcgg
     1681 cgtcgcggaa gaaagccgtc gccgcctggg tgtcggcggc gttgtccagc ttgtactcgg
     1741 aatccgtgga gtagacgatc gagcggcggt cgcgctcgaa gcgatagccg tacgagtccc
     1801 ccgagtgatc ctggcggcgc ggcgtgacgc tccagccggc gatgtcgtac ggtcgcccgg
     1861 gcgccagcgt cacgaactcg atccgcgcgc cgaggcgctc gaaaggtatc gggaagaacg
     1921 gcgcggcgtg ctgactgcgg aacgcgtgct cgagctcacg gtgacagccg tagatgacga
     1981 tgcggttccc gggcaggtaa gcgggcgtga agaatggaaa ccccatgatg tggtcccagt
     2041 gcaggtgcga catgaacaca tggaacacct gcgggctcgt ggcaccgtga cgctgcagca
     2101 cccggttcgc gaagacgcgc gcgccggtgc cgagatcgca gagcacgtat tccgccgcgc
     2161 cggcctcgag ctggacgcag ggtgagtcgc cgccgaaggt ctgcgagagc gccgaacccg
     2221 gcccgcgctc gagccaggcc cgcgccttct cgatcgtccc gacgtcgtgg cccgacgccg
     2281 ccacgagcgc gctggccagg cggtccttga gcgccgcggc atcgaacgcg atcggaaggg
     2341 agccgcgggt gccccagaac ttgacgcgca ccgccggcat cggtcagccg ggcgtgcggg
     2401 cgtcgtacgc ggccagggcg ctcggcgata tcttcgccag cgcctcgcgc acggtgggaa
     2461 agcaatcgaa caccatggtg aagcggctga tctcgaaaat ctcgcggacc accggctgca
     2521 gcgccgccat cacgatggtg ccgccctgct ctcgagactg cctcgcggcg agcatgagtg
     2581 cccgcagccc cacgctgctg atgtagtcga cgcccgaaaa atcgagcacg agccggtcgc
     2641 ctcccgcacg gcactccgcg acgtgcgcgc cgagcccggt cttgaacgag tccgaggtgc
     2701 cgttgtcgat gcgaccgctc agcgtcagga ccttgacgtt cgcgtatcgc ttctgggaaa
     2761 tcagcatgaa gcgtcagctc ccctgtgggc ccgctctctt gtttctcgac tgcattatga
     2821 tgagagcctg gccactgtga ttctgccatg aaactcccgt ccctgagccg cctcgcgaag
     2881 ccctgcgtgg cgttcttcgt cgctctcgcg agcgccggtc ccggcctggc ccagcggctg
     2941 ccgatcgact cgatcaaatt gcccccgggg ttccggatca gcgtattcac cgacaaggtc
     3001 cccgaagcac gctccatgtc gctcggcccc gacggcgtgc tcttcgtggg gacccgcgga
     3061 gaccgcgtct acgccgtgcg ctaccgcggc gacaaggtga ccggcgtcac gaccgtcgca
     3121 tccggcctga acctccccaa tggcgtcgcc ttccgcgacg gcgcgctcta cgtcgccgag
     3181 atcgatcgca tcctgcgctt cgacgacatc gccgcgaagc tcgactcgcc acccaagccg
     3241 gtggtcgtga cggatcgcta tccgggagac cgtgcgcacg gggcgaagct ggtgcgcttc
     3301 ggccccgacg gcctgctgta cgtgccggtc ggcatgccct gcaatgtctg cgagcgcaat
     3361 cccgaccgct acggcgtgat ctcgcgcatc cggcccgacg gctcgggcta cgaggtgttc
     3421 gcccgcggcg ttcgcaacag cgtcggcttc gactgggatc cgcgcacgaa agagctgtgg
     3481 tttaccgaca acgggcgcga catgatgggc gacgacctgc catcggacga gctgaaccac
     3541 gcgcccaagc cggggatgaa tttcggcttc ccctactgcc accagggcga cacgcccgat
     3601 cccgaattcc gaaggcacgg ctgcgccgag ttcgcgccgc ccgcggtcaa gctcgggccc
     3661 cacgtggcgg cgctcgggat gcggttctat accggcgatg cgttcccggc ggagtaccgc
     3721 aacaacatct tcgtcgccga gcacggctcg tggaaccggt cggccaagat cggctaccgc
     3781 gtcgcgcggg tggtcgtcga gggcggccgg gcggtgaagc acgaggtctt cgcccacggc
     3841 tggctgcagg ggcgctccgc gtggggccgt ccggtcgatg tgcagccgac gcccgacggc
     3901 gcgctgctcg tctcggacga cgaggccggg gtgatctacc ggatctcgta ccacaagtaa
     3961 ttgtggtttt gacgtgatct gactcaatat atagtattcc ttggcgcggt atcgtgggca
     4021 cccttgaccc cgacaccgga ccgacgtaga aggacatcat ggacgacacg gcaacgaagg
     4081 tttccaccct gcccgagcag gagatctgcc gcgaggcgct gatcgagaag tacgccaagg
     4141 gcaaggagca gtcgatcgac gacgtccggc ggcgcatcgc gcgcgcgctc gcggccgtcg
     4201 agccacagga ccggcgctcg cactgggaac gccgcttcca tgaggcaatg gaagccggct
     4261 tcatcccggc cggccggatc agttcggcgg ccggcatcca gatgcaggcg acgctcatca
     4321 actgcttcgt gcagcccgtg ggcgattcga tctccgagat cgtcgacggc cggcccggca
     4381 tctacaccgc gctccaggaa gcggcggaga ccatgcgccg cggtggcggc gtcggctatg
     4441 acttctcctc gatccgaccg cggggcgcgg aggtcaaggg cacgcattcc cgggcgagcg
     4501 gcccggtctc gtacatgcgc gtgttcgacc ggtcgtgcga gacggtcgag tcggcgggcg
     4561 cccggcgcgg cgcgcaaatg ggtgtgctgc gctgcgacca tccggacctc gaggacttca
     4621 tccacgccaa ggacaagggc gacctgtcca atttcaacat ctcggtgggc gtcaccgacg
     4681 ttttcatgac ggcggtcgag cgcgacctcg aggtcgagct cgtccacaag gcgaagccgc
     4741 accccgacgc gatcgccgcc ggcgcctacc agcgcgacga cgggctgtgg gtctaccgca
     4801 agctccgcgc gcgcgacctg tgggaccagg tcatgcggtc gacgtacgat cacgccgagc
     4861 ctgggatcct gttcctcgac cgcatcaacc gcgacaacaa tctcaattac tgcgaggcga
     4921 tcgaggcgac gaatccctgc gccgagcagc cgctgccgcc gtacggctgc tgctgcctgg
     4981 gctcgatcaa cctgacgcgc ttcgtgaagg agccgttctc cgagaacgcc gctttcgatt
     5041 tcgagggctt cgcgaagatc gtcgaggtct cggtgcgcat gctcgacaac gtgctcgacg
     5101 tcacggcctg gccgctgaag gcccagcatg aggaggccat gaacaagcgc cgggtgggcc
     5161 tcgggttcac cggcctcggc gatgcgctga tcatgctgcg tctccgctac gacaccgacg
     5221 acgcgcggcg cacggcggct cgcatctccg agtcgatgcg cgatgcggcg tatcgtgcgt
     5281 cgagcgagct cgcgcgcgag cgcggggcgt ttccgctgtt caacgccgac atgtatctgt
     5341 cgggcacgag cttcgccgcg cggctgccgc aggagatcaa gacgctgatc cgcaagcaag
     5401 gcgtgcgcaa cagccacctg ttgtcgatcg cgccgacggg aacgatcagt ctcgccttcg
     5461 ccgacaacgc ctcgaacggc atcgagcctc cgttttcctg gacgtatacc cgcaagaagc
     5521 gcatggccga cggcaccctg aaggaattcc ccgtcgagga ctacgcctgg cggctgtaca
     5581 agcatctcgg cggggacgcc aaggcgctgc cgccgtactt tgtcacggcc ctcgagctca
     5641 cggctgcggc gcacaaggac atggtcgccg cggtggcccc gtcgatcgac acgagcatct
     5701 cgaagacggt gaacgttccc gagaactacc catactccga tttccagggc ctgtacttcg
     5761 cggcgtggaa gtcggggctg aagggtctcg ccacgtaccg gcccaacagc gtgatcggct
     5821 cggtgctgtc ggtcgaagcg cagacgcggc cccaggactt cgtgcagaac gacgtcaacc
     5881 ggcgcatcga gatcaaagcc gtgcccgagc ccgtgctcgc gagcctgcgc tggcccggcc
     5941 ggccgaagct cgcggccggc aacccggcct ggacctacat gatcgagcat ccgcacggcg
     6001 attttgcgct cttcgtcggc cacgtcgaga acgcgcgcgc gcatccgttc gaggcctggg
     6061 taaacggcag cgagcagcct cgcggtctcg gggccgtggc gaagacgctg tcgatggaca
     6121 tgcgtgcgga cgacccggcg tggctgcgcc tcaagctcga tacgctcgcc aagaccgcgg
     6181 gcgacgacgc gttcgacatg ccgttcccgc cgctcggcga gaagaagcgc atgccgagcg
     6241 cctgctcggc ggtggcgcag ctcgtgcgct tccgctgcga gcagctgggt gctctcccgg
     6301 agtcggcgca cgggccggtg atcgatgcga tgttcagcct caaggagccg aagaccggca
     6361 ccgacggcac gatgtcctgg accatcgaca tccacaaccc ggccgcgcgc gacgacttcg
     6421 tcctggggct gaaggagatc acgctgcccg acggcgtgac gcgcccttat tccctatggc
     6481 tctcgggcga gtacccgcgc gcgctcgacg gcctgtgcaa gctgctctcg ctcgacatgc
     6541 gcatcatcga tccggcctgg atcggcatga agctgcgtaa gctgctcgac taccccgagc
     6601 cgctcgggga tttcatggcg ttcgtgcccg ggagccgcaa gcagcagaac tggccgtcga
     6661 ccgtttctta cgtcgcacga ctcgtcatcc atcgctacgc catgctcggg atcctcgacg
     6721 aggacggcat gccggtgcag cagatgggcg tcctggaaac gcccgcgccc ggagccgcgc
     6781 cgaagatgaa gggcttgcgc tgcaccgagt gcggcaacta cgccgtcatc cgcaaggacg
     6841 gctgcgattt ctgcagcgcc tgcggctggg tcggcgtctg cggctgaaga cggctagaag
     6901 tcgttccgcg ctttcgagag cgatttcctg agctcgtcat agcttttcgc gaagccgctt
     6961 ttcacctcgt cccaggcttc ggaggagccg tgcttcaacc cgccgtacca ttcggcgact
     7021 tggttgcgct cgcggcgcat cgcctcgcgc gtggcgcgag cacgctggcg cgccgccgcg
     7081 tccatcttgc cccagtcggc gtcgagcttg cgctcgagcc ggtgcatcct cgcatcggcg
     7141 tcgtcgagcg cggccttggc ctgcttcacc gcctcgtcgc gctgcgcgac gctgtagtcc
     7201 ttgatcgcgt ggccggtgtc cgcggccttc ttcgaaatat ctttcgccgc ggtcctgccg
     7261 gcggatccct ggtccgcgaa ggcggtcgag gccgcgagca gcaagcccag cgacagagcc
     7321 cctatcagac ggccaaaatc tcgcatatcc acgcctcctc cggcggagcg tcagtgtaaa
     7381 acaaaaggcc gcggaggttc gcggccttgg acgaaaccac gattgcccgg tcagctcttg
     7441 aagatgccgt ccaggtgctc gatgtcgttg atgaccttcc actccgacgc gcctgcggcc
     7501 tccacgcgct tttgccaggc gtcgatccgt ccgaggtaac ggcggggatc gatgttgtcc
     7561 gtcttgagct tggtcacgtt caccgcgatc acccggatgt tcttgaggtt gatcgcgaag
     7621 ttgcgcaccg tcgccttgtc gagctcctcc tgcatgtcgg agaagatcag gatggtcttc
     7681 atgcccgccc cggtctcgtt caggaactcg gcgccctgga tcaacccgcc ggtgatgtcc
     7741 gtatacgcgg tgccccttat gccacggaag tagccgtcca tcttgtcctt gaacgcccgc
     7801 ttctgcatgt tcgcctgcga cggcctcgta tcgaagcgcg ccttggcgat gatgtccttc
     7861 tcgctgaagc tgcggctttc gacgcgcgcg atggcgagcg agtcgcccgg ctgcagcgtt
     7921 ccgagcaggt agttgacgat gatctgcgcc ttgttcgcct cccgggcgta cgtgcccgag
     7981 gtatcgacca gcatgtagac cgcattcgta tggctgcgcg ggtcggcgca accggacaag
     8041 gcgacggcgg cggcgaggaa cgacaggaac gcgagaacgg tgcgcttggt catggtcgtc
     8101 tcccgatcgg aagcgggcct aggcgctcat cgaacgcgat ttcacgggca cgccgactcg
     8161 cccgcccttc accaggtgct cgataagcag cggcagcatc accaccacgt catagagggt
     8221 gatgagcacc cggctcgagt gccgcgcgag gttgcccagg acccgcagga cgaaggcgag
     8281 cgcgtgcagg agcgcgacca ccgccacccc cagcaccgtg cgcgaggaat ggatgaacga
     8341 ttcgaggggg atcgcgacga acgccagcgc aaacggcagg atgaagccga gcagcatctg
     8401 gcccgccgtc ggaatacgcc cgacccagcc ctcgttcacg gcggtttgct gctgggacgc
     8461 gagcgtctga aggagcgcct gcttgtcggc gatcagcatg tcgcgcatca gcgccagggc
     8521 cgcctcgatg cccgcgagga tcacgagcag cgtgagcgag atgtaaagga tgcggtgacg
     8581 catcttctcg ttcaggttgg cgatgggcgg gaacagatgc gtgatccgca gcgactccat
     8641 caggaacagg cccatcgacg cctcgaccag gatgatcacc agcgccgcca cctcggacgt
     8701 acgcagcgac tgggtgatgt aatccccggc gccgaccatc tcggacatcg gcagcgcgat
     8761 cagcttgaag ttgacgaagg cgccgcccat cgcgacgaac atgacgatgg tggcgatcgc
     8821 gaactgggtg aaacccgaag tggcgagctg gtgctcggcc ttgtcggtct tcttgttgat
     8881 ctcctcgtac ttcgccatat gggcatcgac tgtaccggcg gtctcctgga tgccggacag
     8941 cttcttgtcg accagcgtca tcgtcttgtc gacggagcgc cagaacggga ggaagccctc
     9001 caggatcttg tggcgcgact cgtaggcgcg gcggtactcc ccgatcgtct tgtcgtgcat
     9061 cttgccgacc gagacattga tctcttccag gatcttctgg acgatctcgt tgccgttacc
     9121 cttcaccttg gcgaccgcag tgaccgcctc cacccactcc ggcggcggcg gcggcacttc
     9181 gccgcacttc ttgtagtcct cctcgatccg ggtgatctcg tcgagtagct tgcgctgcat
     9241 cgccggatag ccctggagat cgcgcgtcac gatcgcgccg atccgctcga actcgcgctc
     9301 gatgcgctgg cccacttcgg cgcgcccgtg cgcgagcagg accgcgcggt tgcgctccct
     9361 gatcccgttg gcagccgcaa ccagcgaccg cgcgcccaag cgcagcggcc cgctcaggga
     9421 gtggccgagc gagcggatga gatcgtgcat gggccggcgt cccgcgtaga gaaatgcgat
     9481 gccgatcagg cacagcgtga tgaccgacag cacggcgttg ttgggccaga tcatccagac
     9541 atgagaatcg accatggcat cccccctgag caggtgtgag agcggctctc cccgaggcag
     9601 acagcataga tcaggcgtcg gggccctccc ccgcaaggca ggtcttttca gggattattt
     9661 ctcgacgctg tgcgtgaaat cgcgcaataa gcaactcgtg tgtcggtctg gggcgacggc
     9721 ccggtgccgc gatgcggcaa cgggcccgct cgcgatatca tcgcgttgcg aatcgaagcg
     9781 cggtcgggac gaggagcggg gaatggggat gaaatggaca ggcttgatgc tgcttgcggc
     9841 tctcggagtc gcgcatgcgc agacagctcc gcccggcgat ccgctctcca cccgattggg
     9901 gtgggaagcc ggtctccagg cgtcccgata ccgctacgag gagcccgatt tcgcgaagct
     9961 gaccgggaat cgcgccggtc tgtccgccgc ctatacggag gccgatggac gcgagttcgg
    10021 ccgactcgag gcgaggtatt cgtacgggtc cctcacgtac cagggctccg gcacgcagaa
    10081 caacgtgccg gaccacatct tcgagttgcg cgccatcggc ggcgccgact accgtcccgt
    10141 ggcgagtgtg tcgctatcgc cgttcgcggg cctgggctat cgctatctct acgacgacct
    10201 gaggggctac gatgtcgtcg ggaatgtcgc gtacttcggc taccgacgct attcccaata
    10261 tctatatgtc ccgctcggcc tgaccgcgcg actgtccgcc ggccgcggct gggtgctcgc
    10321 cccgacgctg gaatacgacg tcttcttacg tggacggcag cagagcaagc tgagcgatgc
    10381 cggattcggg gccccggaca tcacgaacga gcagcggcat ggtcgcggtt atcgcgccgc
    10441 gttcatgatc gaagccggcc acctcgcgtt cggcccgtgg atcgattact ggagcatcaa
    10501 ggactccgag gtctcgaacg gctttctcga gccggccaac cacaccaggg aagcgggaat
    10561 cgaggtgcgt taccggttct gaagggcgct catgggcgcc atgcgccttc gagcaccttc
    10621 tcggcccaca gcagcgccga caaggttttg gcgtcggtga tcttcccctc gcgcacccat
    10681 tgcagcgcgg acgcgagcgg cagggtgaac acctcgagga attcctcgtc gtcgagcttc
    10741 gcttccttcc gcgtcagtcc cgtcgcgagg aagatctcga tgcgctcgtt cgagtagccg
    10801 atcgcgagat gcatcgtggc gagatgacgc cattcggacg cggtgtatcc ggtctcctcg
    10861 agcaactcgc gccgggcagt ctcggcagga ggctcgcctg cgtcgatctt gccggcgggc
    10921 agctcgatca tgtcccgacc gagcggatag cggaactggc gctccatgag gagctcgccc
    10981 tgatcgagca ccgcgatgat caccacggcg ccggggtgcc tgacgtactc acggtcggtc
    11041 acaccgccgt cgggcaggcg caccttgtcg cgccgcacgt ggagcagccg gccgtcgtag
    11101 accacctcgc tcgagagcgt atgctcggtc aggtccttgc gcgggtcggg cattcggcgc
    11161 cggccggtca caggcccttg atcgacagca ggcacagctg catcagcgac tgcggcagga
    11221 tgccgagcgc gagcatcgcc atgccgttca gcgacaggag cacccgcatg tcggccctgg
    11281 tctcgatccg gtgcgcgtcc tgcggttcgt cgaaatacat gagcttcacg atgcgaatgt
    11341 aatagaaggc gccgatgagc gagaacagca ccgccacgac cgcgagccac acctggcccg
    11401 cgtcgagcac gggctggagc accacgacct tggcgtagaa gccgaccgtg ggcggtatcc
    11461 cggcgagcga gaacatcagc agcatcatca cgaaggcgaa ccaggggctg cgcgcgttga
    11521 ggcccttgaa gtcgtcgagg cgctcggctt cgaacccggc ccgcgacagc agcaggatca
    11581 tgccgaacgc cccgaggctc atgagcgcgt agacaatgac gtagaacagg gccgccgaat
    11641 acgcgttcgc ggccgaaaac acgttgccga ccacaccgga cagcagcccg agcagcacga
    11701 accccatgtg cgagatcgtc gagtaggcga gcatgcgctt gatgttcgac tgcgcgatcg
    11761 ccgcgaggtt gccgatggcc atggacagca ccgcgaggat caccagcatg tactgccagt
    11821 tgaaaaccaa gttctgcgac agcgagctga gcaggtcgat ccggccgccg ccctccacga
    11881 acccgagcgt accgttcacc agcatgcgca cgaccatcgc gaacgccgcg agcttggacg
    11941 ccgaaccgat gatgagcgtc accgacaccg gcgcgccgtg atagacgtcc gggatccaca
    12001 tgtgaaacgg cacgacgccg agcttgaagg ccatgcccga taccgcgaac accaggccga
    12061 acagcaggat gatgcgatcc ggctgattcg cgccgatggc cttcgcaacg tcgccgatgt
    12121 acagcgtccc ggtcgcgccg tagatcatgg acatgccata cagcagcaga cccgatgcga
    12181 gcgcgcccaa cacgaaatat ttcatcgcgg cctcggtcga ggccgccgag tcgcggttca
    12241 gcgccaccag cgcatacagc gaaagcgaca tcagctccag accgagatac aggctgagga
    12301 aatgattcgc cgagatcatc accatcatcc cgagcgtcga gaacagcgcc agcgcgaagt
    12361 actcgccgcg gtacaggccg cggtccctga ggtaggcgcg cgagtaaacg agcgaggcgc
    12421 cgaccgcgac gtaacagagc atcttcaaca cgtggcccat ggtgtccgcc acgaacatgc
    12481 gattgaacgc ctcggtgcgt tgcaggccgg tcaagtggcc ggtcaccagc gtccagaacg
    12541 cgcagccggc gagcgccgcc aaggcgagcg cataggtcac atcgcggcgc tgatcgcgca
    12601 cgaacaggtc gacgatcagc acgaccggcg ccatgacgag gagccagagc tccggatatg
    12661 cggggatcag gttcatggct tggtcgcgat gtgcttcaac aggttgtcga ccgacgcgtg
    12721 catgatgtcg gtgaacggcg ccggatgcac gcccatccac agcacggcgg ccgccagggc
    12781 gcccagcacg acgagctcgc gcatcccggc gtcgcgaagc gcggcgacgt ggtcattgcc
    12841 caccgcgccg aagacgacgc gcttgtacat ccagagcgta taagccgcgc cgaacaccag
    12901 cgtcgtggcg gcgaggaatg cgagccagaa attgaccttc atcgacccca tgatgaccat
    12961 gaattctccg acgaagccgc tggtcgccgg cagcccgcag ttggccatcg cgaagagcat
    13021 catcagcgcc gcgaacctcg gcatcacgtt caccacgccc ccgtagtccg cgatattgcg
    13081 ggaatgcatc cggtcgtaca gcacgccgac gcagaggaac agcgcggccg acacgaagcc
    13141 gtgcgagatc atctggacga gcgcgccttc gagggcgagc gggttgaaga ggaacgttcc
    13201 gagggtgacg aaccccatgt gcgagatcga cgagtaggcg atcagcttct tcatgtcggt
    13261 ctgcacgagc gccaccagcc cgatgtacac gatcgccacc agcgacagca cgatcatgaa
    13321 gcccgcgaga tcgtggctcg cgccgggcag gatcgggagc gagaagcgga taaagccgta
    13381 gccgccgagc ttcagcagga tggccgcgag caccaccgag ccgccggtcg gcgcctcgac
    13441 gtgcgcgtcg ggcaaccaag tgtgaaccgg ccacatcggc accttgaccg agaacgccgc
    13501 gaacagcgcg aggaacagca ggatctgcgg gtcgttccag aaatgctcgc cccgcccgat
    13561 gggaacgttg tgccagtcga gaatggagaa gctcccgccc gacttcatgt acaggtacag
    13621 aaaggcgagc agcatcagca gcgatccgag cagcgtgtag aggaagaact tcaccgcggc
    13681 gtagacccgg ttcgggccgc cccatacgcc gatcaccagg tacatcggga tcagcgtcgc
    13741 ctcgaagaag acgtagaaga gcaagccgtc gagcgcggca aacgtcccat tcagcagccc
    13801 cgacatgatc aggaaggcgg cgagatactg tgcgaccttg ttctggaccg actcccaggc
    13861 cgccgcgacc acgagcaccg tgatgaagct gttcagcagc acgaacagca ccgagatgcc
    13921 gtcgacgccg acgcggtact ggacgttgaa gcgcgcgatc cacgtcctgg gcggctccac
    13981 gaactgcatc gcgctcgtgc tcggatcgaa gcccgccacg agcgggagcg tgacgaggag
    14041 gccgatcacc gcgcccgcga gcgcgatcca gcgcgcctcg cgcgaattcc ggtcaccggt
    14101 cgccagaacg atgcaacccg caacgatcgg gatccatatg gccaggctga gccagggagc
    14161 ggacgatgtc attgttgttt tttcagacga gatagcgctg gagccagggc accacgaaga
    14221 cccagacggt gagtagcacg aacgcgccga tgatcatcga gaacgcgtag tcgtagatga
    14281 acccggactg gatccagcgc acgaccccgg cgaaccagcc gacgagcctc gccgatccgt
    14341 tgaccatcag gccgtcgatg aacgtgacgt cgccccagcg ccacaggccg gtcccgagtt
    14401 tgcgcatgcc gcccgcgaaa aaccagtcgt tgaagcggtc gaatccgtac ttctcctcga
    14461 ggatcgtgac gagcaccccc gcgcgagcgc gcacccgggc gggcacctcc ggccgcagga
    14521 tgtacatgaa ccaggcgagc gcgatgccgg cgagcgacag ccagaatggc aggcttccga
    14581 gcgcgtgctg catcatggcg ccgatcccgt ggtactcctc ggccatctcc ctcaggcccg
    14641 aatgatcggg ggaaacatag atcgacccgt cgaaataatt gccgaacagg acggggccga
    14701 cgacccaccc ggcggcgatc gacgggatcg cgagcaggac gagtgggatc gtcaccaccg
    14761 gcggcgattc gtgcggcgcg tggccgtgcc ccgcgtgctc gtgtcccgca tcgccgaaac
    14821 gctccttccc gtggaacgtg tagaacacca gccggaaagt gtagaacgcg gtgacgaaga
    14881 cgcacgcggt caccatgagc cacgcgaacc cggctccggg ggtcttcgac aggtgcacgg
    14941 cctcgatgat cgagtccttc gagaagaagc ccgaaaaccc ggggatggcc gcgttcgcga
    15001 gcgcgccgat gaacacggtt gcgtacgtga ccggcatgta ccgccataaa ccgcccatct
    15061 tgcgcatgtc ctgctcgtgg tgcagggcga tgatcacgga tccggcgccg agaaagagca
    15121 gcgccttgaa gaacgcgtgg gtcatgaggt ggaagatggc cacgggatac gccgacgcgc
    15181 cgagtgcgac cgtcatgtag ccgagctgcg acagcgtcga gtacgcgacc acgcgcttga
    15241 tgtcgtactg gaccagcgcg atcagcgcca tgaagaacgt ggtgatgccg ccgatcacca
    15301 ggacgaacga cagggccgtc tccgacagct cgaacagcgg cgacatgcgg gcgaccatga
    15361 agatccccgc ggtcaccatg gtggcggcgt ggatcagcgc cgagatcgga gtcggaccct
    15421 ccatcgagtc cggcagccag acgtgcagcg ggaactgcgc ggacttgccc atcgcgccga
    15481 tgaagagccc gatgcagatc gcggtgatca gcagccacgg ccagcctggg atggcctcga
    15541 tcgtggcctg cgcgagcgtc ggcgcgcgcg agaacaccgc gccgtagtcc agcgtgccga
    15601 aggtcgcgag caccagggcg actccgatga ggaagccgaa gtcgccgacg cggttcacca
    15661 tgaatgcctt cagattcgcg tagatcgcgc tcggccgcgt gtaccagaac ccgatcagca
    15721 ggtaggacac caggcccacc gcctcccagc cgaaaaagag ctgcaggaag ttgttcgcca
    15781 tcacgagcat cagcatcgag aaggtgaaca gcgcgatgta tgcgaagaac cgctggtatc
    15841 cgggatcgtc ggccatgtag ccgatcgtgt acacgtgcac catcagcgac acgaacgtga
    15901 ccacgaccat catcagcgcc gtgagacggt cgaccaggaa gccgatctcg agccgcaggt
    15961 cccccgacgc gagccaggtg tagagcggct ggttgagcgt ctcgccgttc agcacgcgcc
    16021 cgaggacgac gcacgaagcg atgaaagcga tcgccacgcc caggatcgcg gcccagtgtg
    16081 cgcccgcgcg accgacgcgc ctgccgaaca gcccggcgac gatcgcgccc gcgagcggcg
    16141 ggaagggaac gaggagacaa aggctgtaca ggctcacggt cggctcaccc tcgcagctga
    16201 tccaggtcgt ccacgttgat cgtctggagg ttgcggaaca gcgccaccag gatcgccagc
    16261 ccgatcgccg actcggccgc cgcgacggtg agaatgaaga acacgaacac ctgcccgctc
    16321 atgtcgccca gatagtgcga gaaggcgatg aagttcatgt tgaccgacag cagcatgagc
    16381 tcgatcgcca tcagcaggat gatgacgttc ttgcggttca ggaatatgcc gacgacgctg
    16441 atcgcgaaca gcaccgcgcc cagaatcaag aaatccgaaa gcgacagcaa aacgacctcc
    16501 cttcaacggg gcgcgagcgc cccggacgca cggtctccta gtccctcttt tcggccggca
    16561 tcgacaccag ccggacgcgc tcgctacgcc ggacgagcac ctgcttcgcc gggtcttggt
    16621 acttcgtgtc cttgcgccgc cgcagcgtga gcgcgatcgc ggccacgatc gcgaccagca
    16681 gcagcacggc ggcgagctcg aacgggtacg cgtagtccgt atacagcagg cgccccaacg
    16741 ccttggtgtt gctgaccgcc tgctgcggct gcgcgggcac ggccgactgc agccccgaat
    16801 agcggttcca gagcacgagc cccatctcga tgaccatgag taccccgacc gtcgcgccga
    16861 gcggcaggta gctccagaag ccctcgcgca ggcgatccag attgatgtcg agcatcatga
    16921 cgacgaacag gaacagcacc atgacggcgc cgacgtagac cagcacgagc gtgatcgcga
    16981 ggaactcggc gcgcagcagc acccacagcg ccgccgacgt gaagaaggcc agcaccagga
    17041 agagcgccga gtgcacggga ttgcgcgcgg tgatgacccg cagcgccgac agcaccagga
    17101 tcacggcgaa cacgtagaag gtgaaggtct cgaagctcat cggcgctcag cggtaggccg
    17161 cgtcggcggc gcggtccttc gcgatctgtt cttcgtagcg gtcgccgacc gcgaggagca
    17221 tctccttcgt gtagaccagg tcgccccgct tctccccgtg gtactcgagg atgcgcgtct
    17281 cgacgatcga gtcgaccgga caggattcct cgcacatgcc gcagaagatg cacttggtaa
    17341 ggtcgatgtc gtaccgggtc gtcctccgcg tgccgtcggc acgctggtcc gactcgatcg
    17401 tgatcgcgag cgccgggcag atcgcctcgc acagcttgca cgcgatgcaa cgctcctcgc
    17461 cgttcgggta gcggcgcagc gcgtgcagcc cgcggaagcg cgggctcatc ggcgtcttct
    17521 cctccgggaa ctggatagtg attttgcgcg cgaacaggtt gcgcccggtg agcgccatgc
    17581 ccttcagcag ctcgaccagc aggaaggtct tgaagaaatc gcggatctta tcgagcatgg
    17641 cctatcgcca gggcgaccac ggcgtctgca tccacgcggc gatcaccacc agccagatga
    17701 tcgtcaacgg gatgaacacc ttccagccca gacgcatgat ctggtcgtac cggtagcgcg
    17761 gaaacgtcgc ccggaaccac aggaacatcg tcaccatcac gaatactttc gccaccagcc
    17821 acacgaacgg cggaatcgca ttggtgaaag cgctgtccca caacggcgcc cagccgccga
    17881 gaaacatgat ggtcgcgagc gtcgcgacca ggatcatgtt catgtactcg gccaggaaga
    17941 acagcgcgaa cgtcatgccc gaatactcga cgtggaagcc ggcgacgatc tcggattcgc
    18001 cttcggctac gtcgaaaggc gcccggttcg tctccgcggt gcccgagatg aaatagacca
    18061 ggaacatcgg gaacaacggg agccagttcc aggacagcag attgatgccc ttgtctgcaa
    18121 aaaaaccgcg ctgctgagcg gcgacgatgt cgctgaggtt caggctctgc gacacgagca
    18181 gcacgcatac cagcgcgaat cccatcgcga tctcgtaggc cacgatctgg gcggccgagc
    18241 gcatcgcccc gagaaacgaa tacttcgagt tcgacgccca gccggcgatg atgatgccgt
    18301 agacccccat cgaggtgatc gccatcacgt acagcaggcc ggcgttgatg ttcgccagaa
    18361 cggtctccgg cccgaacggg atcaccgccc acgccgccat cgcgggcatc agcgtgagga
    18421 tcggcgccgc gaagaacagg aagcggttcg cgcccgccgg gacgatgatc tccttcacca
    18481 gcagcttcag cacgtcggcg aacggctgca gcagcccctt cgggcccacg cggttgggac
    18541 cgatccggac ctgcatgtag ccgatgaact tgcgctccca gagcgtgagg taggcgacgc
    18601 acaggatcac cgggatcgat atcgcgagga tcagcagcac cgtcttcacc acgagccagg
    18661 ccggcggccc ccagctcgtc gagaggatcg cgttcgacca gtcggtgagc gtgctcatca
    18721 tgcccgctcg acctcgaccg cggcgaacat cggaccgagc tcggcagtca ggcggtgtcc
    18781 cgccgcgagt cgcacgcagt ccaggggcag gccgtcgtct cggcccgcct cgagcaccgc
    18841 ctggccttcg ccctgccgca cgcgcacgcg gtcgcccgcc gcgaggccga gtcgctgcag
    18901 cgtcgcggaa gccatccacg cgcgcggcgg cagcgcgtcg cgcgtcttct gcagcgatgt
    18961 cgcccggcgg acgatggggt cggtgaaata gatcggcacg tcggcgatgc gccgcaggcc
    19021 gtcgccgcct gattcgaggc tgatctcgac gccgcgcagc gcgttgttga gccggcccgg
    19081 gatggattgc accggacacg cctcgtcgcg caccgcctcg ctcgagtcct gatcgaagcc
    19141 cggcacgccg agcagattgg cgagcacgcg cagcaccttc cacgccggcc gcgcgtcgcc
    19201 gagcggcttc accgctccgt tgaagctctg caggcggccc tcgcagttga cgaagctgcc
    19261 cgacgtctcg gtgaaggggc cgatcggcaa aagcacatcg gcgtactcgg ccgcgaccgt
    19321 gctgccgtaa gcggtcagcg cgaccacgaa atcggccgcg cgcagcgcgc gcagcgccgc
    19381 cgccggatcg tggcaatcga gctcgggttc gaggttgagc agcaggtagg ctttgcgcgg
    19441 ctgctccagc atggactgcg cgttcaagcc gcgcgcgccg gggacgcagt tgacgagata
    19501 gccgccgacg ctgttcgggg cttcgcccag gaaaccgagg ttcacgccga gcaggccggc
    19561 gagctcgtgc gcgagcgcgt gcagctgcgc gctctgcgga tgttgcgtgg cggtgttccc
    19621 gaggaggatc atcgcgttct tgcccgaggc gaggctcgcg gcgatcgcct tcgccccggc
    19681 gcccgccgtg accgccgata cggaggcggg cgcgggcttc tgcgcgatct cggcggccgc
    19741 tttcacaatt tgcgcgagcg cctcgggcag cgcctggggc gcgacgacga gcttgttcgc
    19801 gaccggcatc agcagatcgt cgtcggcgca gtgcagcatg ttgatctcgg cctgccgctt
    19861 cgccgcgccg cgcagccgag ctgcaaccag cgggtggtcc ttgcgcagga acgagcccac
    19921 gaccagcgcc cggtcgagct tgccgaggtc ggccacctgc gcgcccagcc atggcacgcc
    19981 ggcgcgcttg ccgtcgctcg agaaatccga ctgacgcacg cggaagtcga cgttctcgct
    20041 gcccaggccg cgcaccagct tctgcagcag gtgcagctcc tcgagcgtcg aatgcgcgct
    20101 cccgagcgcg ccgatgctct cggcaccgtg ccgcgccttg gcgtccttga ggcgcgccgc
    20161 gacgtgctcg agcgcgggct gccagtcgat ctcgcgccac ttgccgttct ccttgagcat
    20221 cgggcgaagg agccgctcgt cggagttgag tccttcgtac gagaagcggt ccttgtccga
    20281 gagccagcac tcgttcagcg actcgttctc gaggggcagc acgcgcatca ccttgttcag
    20341 cttcacctgg acgatcaggt tcgagcccag gctgtcgtgc gggctcaccg ccttgcggcg
    20401 cgagagctcc caggtccgcg ccgagtagcg gaagggcttg gaggtcagcg cgccgacggg
    20461 gcacaggtcg atcatgttgc ccgagagctc cgaatcgacg gtccgtccga cgaaggagat
    20521 gatctcggcg tgctcgcccc tgccgatcat ccccagctcc atcacgccgg cgatctcctg
    20581 cccgaagcgc acgcagcgcg tgcagtggat gcagcgcgtc atgtcggtcg agatcagcgg
    20641 cccgaggttc ttgttgagaa cgacgcgctt ctcttccgcg tagcgcgagc tcggcttgcc
    20701 gtagccgacg gcgaggtcct gcagctggca ctcgccgccc tggtcgcaga tcgggcagtc
    20761 gagcggatgg ttgatcagca ggaactccat gacgccgttc tgcgcctgcc gcgcgttgtc
    20821 cgagcgtgtg agcaccttca tgccctcggt gaccggagtc gcgcaggcag gcatcggctt
    20881 gggggccttc tcgacctgca ccaggcacat gcggcagttc gccgcgatcg tgagcttctt
    20941 gtgatagcag aaatgcggca cgtagatgcc gagcgcgttc gcggcgtcca tcaccgtggc
    21001 gccgttctgg acttcggtct cgaggccgtc gatcgtgact ttgggcatgg gatcaggccg
    21061 cgcggcgctg cggaacggtg ggcgccgtcg agtagcccgg ctcgctcacc tggcagcgcc
    21121 ggtgatcgat gtggtactgg aactcgtcgc ggaaatgcag gatgaagctc ttcaccggca
    21181 gcgccgcggc atcgccgagc gcgcagatgg tgcggccggc gatgttgtcg gcgacattcg
    21241 tcaggagatc gaggtcctcg ttgcggcctt tgccgtgctc gatgcggtga accatgcggt
    21301 agagccagcc cgtgccctcg cggcagggcg tgcactgccc gcacgattcc tcgaaataga
    21361 aataggacag gcgctcgagc gccttcacca tgcaggtcgt ctcgtccatg acgatgacgg
    21421 cgcccgaccc gagcatcgac cc
//
LOCUS       DATDMW010000339         6245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1344486, whole genome shotgun sequence.
ACCESSION   DATDMW010000339 DATDMW010000000
VERSION     DATDMW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344486"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VI319_14185"
     CDS             <1..354
                     /locus_tag="VI319_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HEY6865043.1"
                     /translation="ARWLPVHGNGVRARLAFDHGEILAAAVARLRAKVEYTSLPAFLL
                     PKEFTLTELQRAYEIVLDRKLEKSAFRTRILAADLVQPVERVREGANRPARMYRLKQR
                     SPVYFARTFSPRNTS"
     gene            436..1419
                     /gene="ugpC"
                     /locus_tag="VI319_14190"
     CDS             436..1419
                     /gene="ugpC"
                     /locus_tag="VI319_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY6865044.1"
                     /translation="MASVSLRGVRKSFGSTEVVHGVDVAIEDGEFCVLVGPSGCGKST
                     LLRMIAGLEEISGGEIAIGARVVNAVAPKERDVAMVFQNYALYPHMTVYENMAFSLKL
                     AGRTKDDVKKRVEDTAQILGLGEYLARYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVAMRTEIKSLHQRLKTTSVYVTHDQIEAMTMADKIVVMNAGRVEQVGSP
                     LELYDDPRNLFVAGFIGSPAMNFMPERRNGRDVLLGVRPEHLDVAADGLRAEVIVVEP
                     TGADTQIFCTVDGTEVTAVVRERHAFHPGEVVHLKPRQTFLFDRASGQRLA"
     gene            1534..2853
                     /locus_tag="VI319_14195"
     CDS             1534..2853
                     /locus_tag="VI319_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY6865045.1"
                     /translation="MSMKRRDFLKMTAGVAAGTAIGTDAFAQKGGATLNLKPEKGARL
                     RVLRWKRFVQGDEDQYMRNVAAFTKKTGIEVRVDAESWEDVRPKAAVAANVGSGPDII
                     LSTFDDANLYPEKLLDVTDVANYLGAKYGGWYDVCRAYLQPDGKRWIGIPLGCAGNAF
                     VYRISQMKEAGFDSFPRDLPGFLKLAKALKAKNHPLGFALGNATGDGNTWTHWLVWAH
                     GGKLVDAKNTVVINSPETIQALEYARELYQTFVPGTLSWLDPNNNKAFLDDQISVTEN
                     GISIYYAAKTSSDPKVKAMAADIGHANLPVGSTGKPTELHLFFNQMIFKYTKYPQAAK
                     AFIAFMMEKEQYAPWQEASIGYVTHPLKAYESNPIWTSDPKHTPYRDCMKIMLPNSYG
                     GKPGYASAQSMADFIIVNMVAEAASGSRSPKDAAARAEQRAQRYYKV"
     gene            2905..3753
                     /locus_tag="VI319_14200"
     CDS             2905..3753
                     /locus_tag="VI319_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY6865046.1"
                     /translation="MLPAGALLVVFLTYPLGLGTWLGFTDARIGRPGSWVGLENFRDL
                     AGDTVAQLSLFNTIFYTVVASVIKFGLGLWLALLLNRHLPFKAFVRAIVLLPFIVPTA
                     LSAIAFWWMYDAQFSVISWALTRMGLIHGYIDFLGDPWLARLSTIAANIWRGVPFVAI
                     CLLAGLQTIPASLYEAAAIDGATPWQKFRHVTLPMLTPIIAVVMTFSVLFTFTDFQLI
                     YVLTRGGPLNATHLMATLSFQRAIPGGNLGEGAALATAMIPFLLAAILFSYFGLQRRA
                     WQQGGR"
     gene            3750..4613
                     /locus_tag="VI319_14205"
     CDS             3750..4613
                     /locus_tag="VI319_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY6865047.1"
                     /translation="MSGDSEGMAYLESLPRRWVTLYAPLAVFLFVLLFPFYWMTMTAF
                     KPDAELMSREANPFWVHGPTLAHFDKLLFHTSYPEWMWNTVIVTVVSTFVSLAASVLA
                     AYAIERLRFRGARPAGLAIFLAYLVPPSILFIPLAAIVFRLGLFDTRWALILTYPTFL
                     IPFCTWLLMGYFRSIPYELEECALIDGATRWQILTRIMLPLAVPGLISAGIFAFTLSW
                     NEFIYALTFVSSSEVKTVPVGVITELVEGDVYHWGALMAGALLGSLPVAVLYSFFVEY
                     YVSGMTGAVKE"
     gene            4610..5308
                     /locus_tag="VI319_14210"
     CDS             4610..5308
                     /locus_tag="VI319_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6865048.1"
                     /translation="MSLDFEERTAIVTGGAQGIGAAIAKRLEAGGAAVRVWDVSGSPK
                     VDVSDPESIAAATKSALRDFGRIDILVNNAGISGPNVPTVDYPLEEWERVLRVNLTGT
                     FLASRAVAPHMVEAGYGRIVNIASVAGKEGNPNAAAYSASKAGVIALTKSMGKELART
                     GVLVNGVAPAAAKTAIFEQMTEAHVAFMLSKIPMGRFVTVDEIAALVCWLASDECSFS
                     TGAVFDISGGRATY"
     gene            5308..>6245
                     /gene="denD"
                     /locus_tag="VI319_14215"
     CDS             5308..>6245
                     /gene="denD"
                     /locus_tag="VI319_14215"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEY6865049.1"
                     /translation="MKVVITGGGGFLGAKLAGALLARGSLAGGSVDRIVLVDQAFPPL
                     PRDPRLETLAGDVSDPALVARAITPDTRAIFHLAAVVSGAAEADFDLGMRVNLGGTRA
                     VLEQARKCTAPPRLVFASSVAAFGGELPEVLDDATAPTPQTSYGTQKVIGEHLVADYS
                     RKGLLDGRSLRLPTIVVRPGKPNAAASSFASAVVREPLNGVLYECPLPPETAVWLLSP
                     RRVVDAFVHALELAPSAWRERRVVNLPGITATVREMIEAMTRIAGPAPAKRITFRVDP
                     RIDAIVRTWPVRFATPRALAMGFKADPDIDTVIRDYL"
BASE COUNT          950 a         2218 c         2070 g         1007 t
ORIGIN      
        1 gcgcgctggc ttcccgtcca tggcaacggc gtgcgcgcgc ggctcgcctt cgatcacggc
       61 gagatcctgg cggcggccgt ggcgcggctg cgcgcgaagg tggaatacac ctcgctaccc
      121 gcgttcctgc tgccgaagga gttcaccctg acggagctcc agcgcgccta cgagatcgtc
      181 ctcgaccgga agctcgagaa gagcgcgttc aggacgcgca tcctcgccgc cgacctggtc
      241 cagcccgtcg agcgcgtgcg cgagggggcg aaccggccgg cccgcatgta ccggctgaag
      301 cagcgatcgc cagtctactt cgcgcgcacc ttcagccccc gcaacacgag ctagggttgt
      361 cccgcctgcc cgcctctcgg cggatgtggc aaactcgctc accgccgtct tccccgggta
      421 tcggctcgcc cccccatggc ctcggtttcg ctccgcggcg tccgcaagtc gttcggctcg
      481 accgaggtcg tccacggcgt cgacgtcgcg atcgaggacg gcgagttctg cgtgctggtc
      541 ggcccgtcgg gctgcggcaa gtcgacgctg ctgcgcatga tcgcgggcct cgaggagatc
      601 tcgggcggcg agatcgcgat cggcgcccgc gtggtcaacg cggtcgctcc caaggagcgc
      661 gacgtcgcca tggtgttcca gaactacgcg ctctacccgc acatgacggt ctacgagaac
      721 atggcgttca gcctgaagct cgccggccgg accaaggacg acgtgaagaa gcgcgtcgag
      781 gacacggcgc agatcctcgg cctgggcgag tacctcgcgc gctacccgcg ccagctctcg
      841 ggcggccagc gccagcgcgt cgccatgggg cgcgcgatcg tgcgcgaccc gcaggtgttc
      901 ctgttcgacg agccgctctc gaacctcgac gcgaagctgc gcgtcgcgat gcgcaccgag
      961 atcaagtcgc tgcaccagcg gctgaagacg acctcggtct acgtcaccca cgaccagatc
     1021 gaggcgatga ccatggccga caagatcgtc gtcatgaacg cggggcgcgt cgagcaggtc
     1081 ggctcgccgc tcgagctcta cgacgatccg aggaacctct tcgtcgcggg cttcatcggc
     1141 tcgccggcga tgaacttcat gcccgagcgg cgcaacggcc gcgacgtgct gctcggcgtg
     1201 cgccccgagc acctcgacgt cgcggccgac ggcctgcgcg ccgaggtgat cgtggtcgag
     1261 ccgaccggcg ccgacacgca gatcttctgc accgtcgacg gcaccgaggt gaccgcggtc
     1321 gtgcgcgagc gccacgcctt ccacccgggc gaggtcgtgc acctgaagcc gcggcagact
     1381 tttcttttcg atcgggcgag cggtcagagg ctcgcatgat gttttgcctt gtcatcctga
     1441 gcgcagcgaa ggatctgctt ttgcggtcgc aaaaggcaaa agcagattct tcgtcgctgc
     1501 gctcctcaga atgacacctt aaaggaggag accatgagca tgaaacgcag ggatttcctg
     1561 aagatgaccg ccggcgtcgc cgccggcacg gcgatcggga cggacgcgtt cgcgcagaag
     1621 ggcggcgcga cgctcaacct caagcccgaa aaaggcgcgc gcctgcgcgt gctgcgctgg
     1681 aagcgcttcg tccagggcga cgaggaccag tacatgagga acgtcgccgc cttcaccaag
     1741 aagaccggca tcgaggtgcg cgtcgacgcc gagagctggg aggacgtgcg cccgaaggcc
     1801 gcggtcgcgg ccaacgtcgg ctcggggccc gacatcatcc tctcgacctt cgacgacgcg
     1861 aacctctatc ccgagaagct gctcgacgtc accgacgtcg ccaactacct cggcgccaag
     1921 tacggcggct ggtacgacgt ctgccgcgcc tacctgcagc ccgacggcaa gcgctggatc
     1981 ggcatcccgc tcggctgcgc cggcaacgcc ttcgtctacc gcatcagcca gatgaaggaa
     2041 gcgggcttcg actccttccc gcgcgacctg ccggggttcc tcaagctcgc caaagcgctg
     2101 aaggcgaaga accacccgct cggcttcgcg ctcggcaacg ccaccggcga cggcaacacc
     2161 tggacgcact ggctggtgtg ggcgcacggc ggcaagctcg tcgacgcgaa gaacaccgtc
     2221 gtcatcaaca gcccggagac catccaggcg ctcgagtacg cgagggagct gtaccagacc
     2281 ttcgtgccgg gaacgctgtc gtggctcgac ccgaacaaca acaaggcctt cctcgacgac
     2341 cagatcagcg tcaccgagaa cggcatctcc atctactacg cggcgaagac ctcgagcgac
     2401 ccgaaggtga aggcgatggc ggccgacatc ggccacgcca acctgccggt cgggtccacg
     2461 ggcaagccga ccgagctgca cctgttcttc aaccagatga tcttcaagta caccaagtac
     2521 ccgcaggcgg cgaaggcctt catcgccttc atgatggaga aggagcagta cgcgccgtgg
     2581 caggaggcct cgatcggcta cgtcacgcac ccgctcaagg cgtacgagtc gaacccgatc
     2641 tggacctcgg acccgaagca cacgccgtac cgcgactgca tgaagatcat gctgccgaac
     2701 agctacggcg ggaagcccgg ctacgcctcc gcgcagagca tggccgactt catcatcgtc
     2761 aacatggtgg ccgaggcggc gagcggctcg cggtcgccga aggacgccgc cgcgcgcgcc
     2821 gagcagcggg cgcagcggta ctacaaggtg tagaggcttt tgcggaagct tctcgactcg
     2881 aagaacgccc tcgggtggct gttcatgctg cccgcgggcg cgctgctcgt cgtctttctc
     2941 acctacccgc tcgggctcgg gacctggctc ggcttcaccg acgcccggat cgggcgcccg
     3001 ggctcgtggg tgggcctcga gaacttccgc gacctcgcgg gcgacaccgt cgcgcagctg
     3061 tcgctgttca acacgatctt ctacaccgtg gtcgcgagcg tgatcaagtt cggactcggc
     3121 ctgtggctcg cgctgctcct caaccggcac ctgccgttca aggcgttcgt ccgcgcgatc
     3181 gtgctgctgc ccttcatcgt gccgaccgcg ctctcggcga tcgcgttctg gtggatgtac
     3241 gacgcgcagt tctcggtgat ctcgtgggcg ctcacgcgca tggggctgat ccacggctac
     3301 atcgacttcc tcggcgatcc ctggctcgcg cgcctgtcga ccatcgccgc caacatctgg
     3361 cgcggggtgc ccttcgtcgc catctgcctg ctcgccgggc tgcagacgat cccggcttcc
     3421 ctctacgaag ccgccgcgat cgacggcgcg acgccgtggc agaagttccg gcacgtgacg
     3481 ctgccgatgc tgacgccgat catcgccgtg gtgatgacct tctcggtgct gttcaccttc
     3541 accgacttcc agctgatcta cgtgctcacg cgcggcggcc cgctcaacgc cacgcacctg
     3601 atggcgacgc tctcgttcca gcgcgcgatc cccggcggca acctcggcga aggcgccgcg
     3661 ctcgcgaccg cgatgatccc gttcctgctc gcggcgatcc tgttctcgta tttcgggctg
     3721 cagcggcggg cgtggcagca ggggggacga tgagcggcga cagcgagggg atggcgtatc
     3781 tcgagtcgct cccgcggcgc tgggtgacgc tgtacgcgcc gctcgcggtg ttcctgttcg
     3841 tgctgctgtt cccgttctac tggatgacga tgacggcgtt caagcccgac gccgagctga
     3901 tgagccgcga ggcgaacccg ttctgggtgc acgggccgac gctcgcgcac ttcgacaagc
     3961 tcctgttcca cacctcgtac cccgagtgga tgtggaacac ggtgatcgtc acggtggtct
     4021 cgaccttcgt ctcgctcgcc gcgagcgtgc tcgccgcgta cgcgatcgag cggctgcgct
     4081 tccgcggcgc gcggcccgcg gggctcgcga tcttcctcgc gtatctcgtg ccgccgtcga
     4141 tcctgttcat cccgctcgcg gcgatcgtgt tcaggctcgg gctgttcgac acgcgctggg
     4201 cgctgatcct cacctacccc acgttcctga tcccgttctg cacctggctg ctgatgggct
     4261 acttccgctc gatcccctac gagctcgagg agtgcgcgct gatcgacggc gcgacgcgct
     4321 ggcagatcct gacgcgcatc atgctgccgc tcgcggtgcc gggcctgatc tcggcgggca
     4381 tcttcgcctt cacgctgtcg tggaacgagt tcatctacgc gctcaccttc gtgtcctcgt
     4441 cggaggtgaa gacggtgccg gtcggcgtca tcaccgagct ggtcgagggc gatgtctacc
     4501 actggggcgc gctcatggcg ggcgcgctgc tcggctcgct ccccgtggcc gtcctctact
     4561 cgttcttcgt cgagtactac gtctcgggaa tgaccggcgc ggtgaaagaa tgagcctcga
     4621 tttcgaggag cgcaccgcga tcgtcaccgg cggcgcgcag ggcatcggcg cggcgatcgc
     4681 gaagcgcctc gaggcgggcg gcgcggcggt gcgcgtgtgg gacgtgagcg gctcgccgaa
     4741 ggtcgacgtt tcggatcccg agtcgatcgc ggccgcgacg aagagcgcgc tgcgcgactt
     4801 cggccgcatc gacatcctgg tcaacaacgc cggcatctcc ggcccgaacg tgccgaccgt
     4861 cgactacccg ctcgaggagt gggagcgcgt gctgcgcgtc aacctcaccg ggacattcct
     4921 cgcctcgcgc gcggtcgcgc cgcacatggt cgaggccggc tacgggcgca tcgtcaacat
     4981 cgcctcggtc gcgggcaagg aaggcaaccc caacgccgcc gcgtactccg cgtcgaaggc
     5041 cggcgtgatc gcgctcacca agtcgatggg caaggagctc gctcggaccg gcgttctcgt
     5101 gaacggcgtg gcgcccgccg cggcgaagac cgcgatcttc gagcagatga ccgaggcgca
     5161 cgtcgccttc atgctctcga agatccccat ggggcgcttc gtgacggtcg acgagatcgc
     5221 cgcgctggtc tgctggctcg cctccgacga gtgctcgttc tcgaccggcg ccgtgttcga
     5281 catctcgggc ggaagggcga cgtactgatg aaagtcgtga tcaccggagg cggcgggttc
     5341 ctcggcgcga agctcgccgg agcgctgctc gcgcgcggct cgctcgccgg cggcagcgtc
     5401 gaccgcatcg tcctcgtcga ccaggcgttc ccgccgctgc cgcgggaccc gcgcctggag
     5461 acgctcgcgg gcgacgtctc cgatccggcg ctcgtcgcgc gcgcgatcac gcccgacacg
     5521 cgcgcgatct tccacctcgc cgcggtcgtc tcgggcgcgg ccgaggccga cttcgacctc
     5581 ggcatgcgcg tgaacctcgg cggcacgcgc gcggtgctcg agcaggcgcg aaaatgcacc
     5641 gcgcctccgc ggctggtgtt cgcgagctcg gtcgccgcct tcggcggcga gctgcccgag
     5701 gtgctcgacg acgcgacggc gccgacgccg cagacctcgt acggcacgca gaaggtgatc
     5761 ggcgagcacc tggtcgccga ctactcgcgc aagggcctgc tcgacgggcg ctcgctgcgc
     5821 ctgccgacca tcgtcgtgcg cccgggaaag ccgaacgccg ccgcgtcctc gttcgcgagc
     5881 gcggtcgtgc gcgagccgct gaacggagtc ctctacgagt gcccgctgcc gcccgagacc
     5941 gcggtgtggc tgctgtcgcc gcgccgcgtg gtcgacgcct tcgtccacgc gctcgagctc
     6001 gcgccctcgg catggcgcga gcgccgcgtc gtcaacctcc cgggcatcac cgcgaccgtg
     6061 cgcgagatga tcgaggcgat gacgcggatc gccgggccgg cgcccgcgaa gcggatcacc
     6121 ttccgcgtcg atccgcgcat cgacgcgatc gtgcgcacct ggccggtgcg cttcgccacg
     6181 ccccgcgccc tcgcgatggg gttcaaggcc gaccccgaca tcgacaccgt gatccgcgac
     6241 tacct
//
LOCUS       DATDMW010000340         4876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_969597, whole genome shotgun sequence.
ACCESSION   DATDMW010000340 DATDMW010000000
VERSION     DATDMW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969597"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VI319_14220"
     CDS             <1..646
                     /locus_tag="VI319_14220"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY6865050.1"
                     /translation="LWRQAKVGLEYLLHRTGPLTVAGGYAGAFTRTRADLARPDAQIY
                     FINFSTAKRGGVLHRHSGFTLAVSQCQVESRGAVAIRSADPGEAPAIRYNYLATENDR
                     RMMVGGLKIVRRIAQTQPLAGYVAGEVLPGPAVASDDEWLDFCRRAGETVFHPTSTCA
                     IGRVVDARLKVRGVEGLRVIDASVMPAVPSGNINATVIAMAEKGADLVKEDARR"
     gene            646..954
                     /locus_tag="VI319_14225"
     CDS             646..954
                     /locus_tag="VI319_14225"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HEY6865051.1"
                     /translation="MWESLALLVLAAGALLWADSLRARERAVRAGRSACERYRLQFLD
                     DTVSFARMRLARDDGGQLKISRTYTFEFSDTGNNRRHGAIVMLGGDVADLHMEPYRMQ
                     "
     gene            951..1676
                     /locus_tag="VI319_14230"
     CDS             951..1676
                     /locus_tag="VI319_14230"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEY6865052.1"
                     /translation="MSDTWLSTKVVENRHWTENLFSLRVEGGSLAFRAGQFVRIALDV
                     EGQRVARAFSFVNPPQDPVLEFYGIIVPEGPLSPRLARLRAGDTLYVAPNPAGFLVLD
                     EVPQADTLWLVATGTGLAPFLSILRTDEPWRRFRNVVLVHAVRHAHELAYRELIAGTR
                     ARYVTFVSREAHAGSLAGRIPAAVRDGRLESAAAVAIDERSHFMLCGNPQMLRDMTEA
                     LAARGLRKHRRRAPGHISVESFW"
     gene            1850..>4876
                     /locus_tag="VI319_14235"
     CDS             1850..>4876
                     /locus_tag="VI319_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6865053.1"
                     /translation="MNEPKPIVGLEGRADSVGYAGRTRYTQLEYEALLANASIGIAFT
                     RERRFFLCNPRFAELFGYRPEELIGQPGEMIYASEASYQALAQIAVPVLASGRQLDTE
                     WEMRRRDGTPFLCRLIAKAIDPANTQQGTVWIVDDITERKRHADEVARLLREQEAILG
                     TATIGIVFVKDRRIVRCNRRYEEMYGYGPGEMNGQFTSILYPDARAYAEGGGVYRTLA
                     RGETSRRVELRRRKDGTTFWNRADGRAVDPNDPHKGSVWIVEDVTDQQRDAEAVQRLL
                     VEQEALLNNVVVGIHFTRDRRTVRCNRRFEEMFGYAPGAAIGAPTRAFYFSDAEYEEI
                     GRRYGELDRGETHSREAWLRRQDGSGFWARITGRAVQPGDPAKGYVWLIEDITERRRS
                     DEALERLVREQAEAEARIERAAAEQELILDNAAVGIAFVRGRVIQRCNRFLEEMVGAQ
                     PGELAGQSSAVLFAERDDWEKAGALAYLTTPPGGTHDAEWRFRRRDGSEFRCRNRGRR
                     TDAGDEIQEWIWSFEDVTAEREAEVRVQRALAEQELILGNATVGITFSRNRVYQRCNR
                     RFEEMLGYGPGELIGLAAEVVYADAEDFRGDGLLYDDLRAGKAVSVERLLRRRDGSTF
                     WCKLVGRAIDPARPLEGAIWIYDDVDAEHQARQSLEASRAALERAVAERTVELEEAKA
                     RAQHLADHDPLTGLPNRRLLEDRLTQALALSQRNGKQTAVMFVDLDRFKPINDTLGHA
                     AGDRLLKEISQRLVSQLRVGDTICRIGGDEFIVVLPELKRPGDVAQVAQKVIDQLSQP
                     VLVDERELVVSCSIGIAVFPDDGRDAETLIRNADAAMYHAKELGRANYQFFTEKMNVA
                     AARRLTLENDLRRAVARGELRVYYQPFIETATGRVVGHEALVRWHHPERGVVMPSEFI
                     PLAEETGLILGIGAWVLREACRWATFIGAPSHNGGAGLQVAVNLSPRQFNDPKLGQMV
                     AQVLQESGLPPRLLEIEVTETLAMTHSDAALATLKKL"
BASE COUNT          735 a         1739 c         1742 g          660 t
ORIGIN      
        1 cctgtggcgc caggcgaagg tggggctgga atatctgctg cacaggaccg gcccgctcac
       61 cgtggcaggc ggctatgccg gcgcattcac gcgcacgcgc gccgatctcg cgcgccccga
      121 tgcgcagatc tatttcatca acttctccac cgcgaagcgc ggcggcgtgc tgcaccggca
      181 ctccggcttc acgctcgccg tgtcgcagtg ccaggtcgag tcgcgcggcg cggtggcgat
      241 ccgctcggcc gatcccggcg aggcgccggc gatccgctac aactacctcg cgacggaaaa
      301 cgaccgccgg atgatggtcg gcggactgaa gatcgtgcgc cgcatcgcgc agacgcagcc
      361 gctcgccggc tacgtcgccg gggaagtcct gcccgggccc gcggtggcga gcgacgacga
      421 gtggctcgac ttctgccggc gcgccggcga gacggtcttc cacccgacct ccacgtgcgc
      481 catcggccgc gtcgtcgatg cgcggctgaa ggtccgcggc gtggaagggc tgcgcgtgat
      541 cgacgcgtcg gtgatgcccg ccgtgccctc gggcaacatc aacgcgacgg tgatcgcgat
      601 ggcggagaaa ggcgccgacc tcgtgaaaga ggacgcgcgc cgctgatgtg ggaaagcctc
      661 gcgctgctcg tcctcgccgc cggcgcgctg ctctgggccg acagcctgcg cgcgcgggag
      721 cgcgcggtgc gcgccggacg ctcggcatgc gagcgctacc ggctgcagtt cctcgacgac
      781 accgtgtcct tcgcgcgcat gcgccttgca cgcgacgacg ggggacagct gaagatctcg
      841 cgcacgtaca ctttcgagtt ctcggacacc ggcaacaacc ggcggcacgg cgcgatcgtc
      901 atgctgggcg gcgacgtcgc cgatctgcac atggagccgt accgaatgca atgagcgata
      961 cctggctcag caccaaggtc gtcgagaacc gccattggac ggagaacctg ttctcgctcc
     1021 gcgtcgaggg cggcagcctc gctttccgcg ccgggcagtt cgtgcgcatc gcgctcgatg
     1081 tcgagggaca gcgcgtcgcg cgcgccttct ccttcgtgaa cccgccccag gacccggtcc
     1141 tcgagttcta cggcatcatc gtgccggaag gcccgctctc gccgcgcctc gcacgcctgc
     1201 gcgcgggcga tacgctctac gtggcgccca atccggccgg cttcctcgtc ctcgacgagg
     1261 tcccgcaggc cgacacgctg tggctcgtcg ccaccggcac cggcctcgcg ccgttccttt
     1321 ccatcctgcg caccgacgag ccgtggcggc gcttcaggaa cgtcgtgctg gtccacgcgg
     1381 tgcgccatgc gcacgagctt gcgtaccgcg agctgatcgc cggcacgcgc gcgcgctatg
     1441 tgactttcgt cagccgcgag gcgcacgccg gctcgctcgc cggccgtatt cctgcggcgg
     1501 tgcgcgacgg gcgcctcgag tcggcggccg cagtggcgat cgacgagcgc tcgcacttca
     1561 tgctgtgcgg taatccacag atgctgcgcg acatgaccga ggcgctcgcc gcgcgcgggc
     1621 tgcgcaagca tcgccgccgc gcgccggggc acatcagcgt cgaaagcttc tggtagccgg
     1681 cgagcgtaag aaatcgtcgg tgaaactgtg tccggcgatg ctcacagggc ctgtcgcggc
     1741 gcggcctctt ttaagggtac gatcccacgg ggatcggtac aagaaaaagg ggctcggggg
     1801 tcgcggtggc gaggagggtt tccacgcagc gcagcgcgcg ggtggcgcgg tgaacgagcc
     1861 gaagccgatc gtcggcctcg aagggcgcgc cgacagcgtc ggctacgccg ggcgcacgcg
     1921 ctacacgcag ctcgagtacg aggcgctgct cgccaacgcc tcgatcggca tcgccttcac
     1981 gcgcgagcgg cgcttcttcc tgtgcaatcc gcgcttcgcc gagctgttcg gctaccgccc
     2041 cgaggagctg atcggccagc cgggcgagat gatctacgcg agcgaggcga gctaccaggc
     2101 gctcgcgcag atcgccgtgc cggtgctcgc ctccggccgc cagctcgaca ccgagtggga
     2161 gatgcgccgc cgcgacggca cgccgttcct ctgccgcctg atcgcgaagg cgatcgatcc
     2221 ggccaacacc cagcagggca cggtctggat cgtcgacgac atcaccgagc gcaagcgcca
     2281 cgccgacgag gtcgcccgcc tgctgcgcga gcaggaggcg atcctcggca ccgcgacgat
     2341 cggcatcgtg ttcgtcaagg accggcgcat cgtgcgctgc aaccggcgct acgaggagat
     2401 gtacggctac ggccccgggg agatgaacgg ccagttcacc tcgatcctct atccggatgc
     2461 gcgcgcctac gccgaaggcg gcggcgtgta ccggacgctc gcgcgcggcg agacctcgcg
     2521 ccgcgtggag ctgcgccggc gcaaggacgg caccacgttc tggaaccgcg ccgacgggcg
     2581 cgcggtcgac ccgaacgatc cgcacaaggg gtcggtgtgg atcgtcgagg acgtcaccga
     2641 ccagcagcgc gacgcggagg cggtgcagcg cctgctcgtc gagcaggagg cgctgctcaa
     2701 caacgtcgtc gtcggcatcc acttcacgcg cgaccggcgt accgtgcgct gcaaccggcg
     2761 cttcgaggag atgttcggct acgcgccggg cgccgcgatc ggcgcgccca cgcgcgcctt
     2821 ctatttcagc gacgccgagt acgaggagat cggccgccgc tacggcgagc tggaccgcgg
     2881 cgagacgcac tcgcgcgagg cctggctgag gcgccaggac ggctcgggct tctgggcgcg
     2941 catcaccggc cgcgcggtgc agccgggcga tccggccaag ggctacgtct ggctgatcga
     3001 ggacatcacc gagcggcgcc gctccgacga ggcgctggag cgcctggtgc gcgagcaggc
     3061 cgaggccgag gcgcgcatcg agcgcgcggc ggccgagcag gagctcatcc tcgacaacgc
     3121 cgcggtcggc atcgccttcg tgcgcgggcg cgtcatccag cgctgcaacc gcttcctcga
     3181 ggagatggtc ggcgcgcagc cgggcgagct cgccgggcag agctcggcgg tgctcttcgc
     3241 cgagcgcgac gactgggaga aggccggcgc gctcgcctac ctgaccacgc cgccgggcgg
     3301 cacgcacgac gccgagtggc gcttccggcg gcgcgacggc agcgagttcc gctgccgcaa
     3361 ccgcggccgg cgcaccgacg cgggcgacga gatccaggag tggatctgga gcttcgagga
     3421 cgtgaccgcc gagcgcgagg ccgaggtgcg cgtgcagcgc gcgctcgccg agcaggagct
     3481 catcctcggc aacgcgaccg tcggcatcac gttcagccgc aaccgcgtct accagcgctg
     3541 caaccgccgc ttcgaggaga tgctcggcta cgggcccggc gagctcatcg gcctcgcggc
     3601 cgaggtggtg tacgcggacg ccgaggattt ccgcggcgac ggcctgctct acgacgacct
     3661 gcgcgccggc aaggcggtct cggtcgagcg gctcctgcgc cgccgggacg gcagcacgtt
     3721 ctggtgcaag ctcgtgggac gcgccatcga cccggcgcgc ccgctggagg gcgcgatctg
     3781 gatctacgac gacgtcgacg ccgagcacca ggcgcgccag tccctcgagg cgtcgcgcgc
     3841 cgcgctcgag cgcgccgtcg ccgagcgcac ggtggagctc gaggaggcga aggcgcgcgc
     3901 gcagcacctc gccgaccacg atccgctcac cggcctgccg aaccggaggc tcctggaaga
     3961 ccggctcacg caggcgctcg cgctcagcca gaggaacggc aagcagaccg cggtgatgtt
     4021 cgtcgacctc gaccgcttca agccgatcaa cgacacgctc ggccacgcgg ccggcgaccg
     4081 cctgctcaag gagatctcgc agcgcctggt gagccagctg cgcgtcggcg acacgatctg
     4141 ccgcatcggc ggcgacgagt tcatcgtcgt gctgcccgag ctcaagcgcc cgggcgacgt
     4201 ggcgcaggtg gcgcagaagg tgatcgacca gctatcgcag ccggtgctcg tcgacgagcg
     4261 cgagctcgtc gtgtcgtgct cgatcggcat cgccgtcttc cccgacgacg gccgcgacgc
     4321 cgagacgctg atccgcaacg ccgacgcggc gatgtaccac gcgaaggagc tcggccgggc
     4381 gaactaccag ttcttcaccg agaagatgaa cgtcgccgcg gcgcgccgcc tcacgctcga
     4441 gaacgacctg cgccgcgccg tcgcgcgcgg cgagctgcgc gtgtactacc agccgttcat
     4501 cgagaccgcc accggccgcg tggtcggcca cgaggcgctc gtgcgctggc accatcccga
     4561 gcgcggcgtg gtgatgccga gcgagttcat cccgctcgcc gaggagaccg gcctcatcct
     4621 cggcatcggc gcgtgggtgc tgcgcgaggc gtgccgctgg gcgaccttca tcggcgcgcc
     4681 gagccacaac ggcggcgccg ggctgcaggt cgcggtgaac ctctcgccgc gccagttcaa
     4741 cgacccgaag ctcggccaga tggtggcgca ggtgctgcag gagtcggggc tgccgccgcg
     4801 gctgctcgag atcgaggtga ccgagacgct ggcgatgacg cacagcgacg cggcgctcgc
     4861 gacgctgaag aagctg
//
LOCUS       DATDMW010000341        10771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_348425, whole genome shotgun sequence.
ACCESSION   DATDMW010000341 DATDMW010000000
VERSION     DATDMW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348425"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /locus_tag="VI319_14240"
     CDS             complement(<1..1725)
                     /locus_tag="VI319_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY6865054.1"
                     /translation="MSEPDAIADIAQRRRALAPDGSFIVQAPAGSGKTELLIQRYLVL
                     LARVRQPEEIAAITFTKKAAAEMRGRVLEALARARREPRPPEPHRALTWESAAAVLER
                     DARLGWRLEEQPGRMRIQTMDALCLSLTRQMPLLGGAGTTPGVVEDATAHYREAARAT
                     LAMIDDADAGAEVAALMAHLDNDFAQAETLLASMLARRDQWLRKYGAPDRARLESALA
                     AVRDEQVERVRSLWPEDVPPRLGRDAAVEAWRKLGEDLVVQKGTWRKPVPPALEALDE
                     LRAALHALRAVPPARYTDAQWETLGAISRLLRLAAAQLKLVFLERGQVDFIEIAAGAI
                     AALGASEAPTDLLLALDYRIQHLLVDEFQDTSFTQFELLERLTSGWQPGDGRTLFLVG
                     DPMQSIYRFREADVGLFLRARHQGIGAVTLEPLTLSVNFRSQADIVDWLNTAFRRVLP
                     RDEDFAAGAVPYTPSDWVHPAEPDAVTVHARFDRDPEAEAADVAALARAEGTVAILVR
                     SRGHLERIVPALKRAGLRYRAIEIDPLERRQAVQDLLALTRALCHSGDRLAWLSVLRA
                     PWCGLRLDD"
     gene            complement(1722..4481)
                     /locus_tag="VI319_14245"
     CDS             complement(1722..4481)
                     /locus_tag="VI319_14245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY6865055.1"
                     /translation="MNGETPFPEISREALLRRLMEAGSAGPTVVTPNARLAAALKRDY
                     DLRQSGRGLASWETADILPLPALVERLYEDLLYSDRATALPALLSGPQEQLMWETVIG
                     DTAAGRSLLAAAPAAALAREAWELAHAWRLSHRLREFPGSDDARAFAEWAWRYEGLTA
                     RDRLVDRARLPDLVASNLDRQSDVVAYGFDILTPQARDFLGALAAAGARVEMSAAEAR
                     PGTSRRLAFESATDEIAAAARWARQRLESAARAGAAHVRIGVVVPDLARAREAVRRIF
                     SETLAPGGTIASSDETPAPPFNISLGEPLSAYPLVAVAMIAFDMARGVASGARIELER
                     ASRLVRSPFIAGAETELDARARLDAELRRRLRRTVGVDALRKVIERATATDNRWRVAE
                     CPVLTRCLSDLAEVAKAELTGTKRPGEWARALLRVLGALGFPGERPLSSAEHQALDKL
                     HQAITELASLDRVAGRVRFGEVLARFQTMIEGVLFQPQTPDVPVQILGVLESAGLEFD
                     HLWVAGLTDEAWPLRSSPNPLLPIVLQRAAGIAQASPAAALELDVRLTRAWLTAAPEV
                     IVTHAQREEDRDLLPSPLIRDVPAVAPEDLQLPQSATATQAIRAARRMERIEDAHAPA
                     LVGTAVVAGGTGVFRDQAACPFRAYAAHRLRADALEPLCSGPDASDRGRLVHAMLEKL
                     MGELDSKAKLDAASGRDLEERLAAAADYAINKQRYAGEASIDGRTARVERARLVRLGR
                     EWLEKEKQREPFDVVAREQKRAVTFGGVTVNVKLDRMDRLQGAPGGRAILDYKTGPVS
                     VSGWLGPRPDEPQLPLYALGGDEPIAAVAFACVKAGAMRFAGISSAGEPIPGVCEVTR
                     QRVKAAEDYRSWDELLRRWRAELDALGREFAAGDARVAPKHGDDTCEQCGRRALCRIN
                     ERELS"
     gene            complement(4538..5782)
                     /locus_tag="VI319_14250"
     CDS             complement(4538..5782)
                     /locus_tag="VI319_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6865056.1"
                     /translation="MSAILTASAAPTRSFREVWLISMGHTLTHWYPATFYLLLPLIGK
                     ELGLSYSQIGLIMTCQSIAAAVSNIPGGMFVDTVGRKGFLLALSLFWVGFPYLLMGFT
                     HTYWLLLVCISLVGIGNNLWHPTAIPTLASRFPARKGLVLSIHGMGGNVGDAVAPLVI
                     GALLATLTWRQVVFVNVAPGVVIAGLILVYLGSLRLAGEASERKHPRDGQTLGEYFQG
                     LRELLKNRKMILLTVSSSFRTMTQTGLLTFLPLFLAYELHYSPLWVGACMFMLQAAGF
                     AASPIAGHLSDRMGRRSILMSSMGMTGVVLLFMAIAGRSSAFIFFIAVLGFFLYAVRP
                     VLQAWILETTPKKMGGTSIGVLFGAQHIGSALGPVLGGVIADRYGIMAAFYFLAATIV
                     VANLFIFFIPARDEPKQDAAAS"
     gene            complement(5797..7464)
                     /locus_tag="VI319_14255"
     CDS             complement(5797..7464)
                     /locus_tag="VI319_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEY6865057.1"
                     /translation="MPQDKRTHTLADHVYGEDYGHTHDGDEPFDHDHDHDDFGPDSPG
                     PDPLWMQDNVSLLSVGMDIGSSGTQVVFSRLNLRRLAEDLTSRYFVVSRETLYQSPVS
                     LTPYQSETLIDEAKLGDIIDRAYEDAKLHPDNIDAGVVILTGEALRRENAEAIAGLLA
                     EQGGEFVCATAGHHMEAMLAAYGSGAAAASHDGERRILNVDIGGGTTKLALVERGRVT
                     ATAAIHVGGRLTVVDERGVIVRLDPTGRRHAADAGFDWRLGDTASREDRARVAESLAD
                     TLVSALAPRSARPALASLWLTEPLPWPEDGVDGVMFSGGVAEYVYGRETRDFGDLGRL
                     LGQALRRRLDSGALPWPLLPPGECIRATALGASEYSVQLSGNTIFISDHAALLPRRNL
                     QVLQPPFRFGAEIDERALVKAVRGHFTAFDLTEGETEVALAFRWQGPPLYERVAAFAR
                     ALVAALPATIAEARPLYIVLDGDIAQTLGHILRDEMRVASEILVIDGVTLWDFDYVDL
                     GRIRLPSNTVPVTVKSLVFKADPRLPHGHPHGHHHDHDHGHHHHHET"
     gene            complement(7553..7876)
                     /gene="fdxA"
                     /locus_tag="VI319_14260"
     CDS             complement(7553..7876)
                     /gene="fdxA"
                     /locus_tag="VI319_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY6865058.1"
                     /translation="MAYVVTESCIKCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCT
                     LCVPECPVEAIFAEDDVPPEQRAFIATNAELAKIWKPIVERKEAPGDADEWAKVKDKK
                     NLLER"
     gene            8126..8452
                     /gene="trxA"
                     /locus_tag="VI319_14265"
     CDS             8126..8452
                     /gene="trxA"
                     /locus_tag="VI319_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY6865059.1"
                     /translation="MSDLIHHVSDNTFEPEVLKSDVPVLVDYWAEWCGPCKAIAPVLD
                     EVAKEYSGKLKVAKVNVDENQEIPRKYGIRGIPTLMLFKNGNVEAQKVGALSKSQLTA
                     FLDSNI"
     gene            8622..9881
                     /gene="rho"
                     /locus_tag="VI319_14270"
     CDS             8622..9881
                     /gene="rho"
                     /locus_tag="VI319_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY6865060.1"
                     /translation="MHLSELKSLHVSALLDMATQNEIDGANRMRKQELIFALLKNRAK
                     KGESIFGDGTLEVLPDGFGFLRSPDTSYLASTDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKDGERYFALVKVDKVNADSPEASKHKILFENLTPLHPDEQLKLERDIKAEENITSR
                     IIDIIAPIGKGQRGLIVSPPKAGKTVLMQQLAHAITANNPDATLIVLLIDERPEEVTE
                     MTRTVRGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIFEE
                     FKGTGNMELHLDRRMYEKRIFPAINVNRSGTRREELLIEAPILQKVWVLRKLLYPMDD
                     LEAAEFLVDKIKATKSNGDFFDSMRRG"
     gene            10005..10238
                     /gene="rpmE"
                     /locus_tag="VI319_14275"
     CDS             10005..10238
                     /gene="rpmE"
                     /locus_tag="VI319_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY6865061.1"
                     /translation="MKAKVHPEYKEVQVTCSCGNTFVTRSTLGKPLHVEVCSACHPFY
                     TGKQKIVDTAGRVEKFRQKYAGKKTIKAKQASA"
     gene            10321..>10771
                     /locus_tag="VI319_14280"
     CDS             10321..>10771
                     /locus_tag="VI319_14280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY6865062.1"
                     /translation="MRLPSAPVVLPLAPALLVLVAAAYVLPGVLAHDPWKTEDAVGIG
                     IVHQMLVHGQWLIPHLAGEPYFDDGPLYYWLAALCAKIASPILAVHDGARLASALAMA
                     ASLACVYHTGRELYDRAAGTAAALVLLGSLGVLVHAHETLAEMGMLAA"
BASE COUNT         1692 a         3750 c         3780 g         1549 t
ORIGIN      
        1 gtcgtccagc cgcagcccgc accagggcgc gcgcagcacc gagagccacg cgagccggtc
       61 gccggaatgg cagagcgcgc gcgtcagcgc gagcaggtcc tgcaccgcct gacgccgctc
      121 gagcgggtcg atctcgatcg cgcggtagcg caatcccgcg cgcttgagcg cggggacgat
      181 gcgctccagg tggccgcggc tgcgcacgag gatcgcgacc gtgccttcgg cgcgggcgag
      241 cgcggccacg tctgcggcct cagcctcggg gtcgcggtcg aaacgcgcgt gcacggtgac
      301 cgcgtccggc tccgcgggat ggacccagtc cgagggcgtg tacggaaccg cgcccgctgc
      361 gaaatcctcg tcgcggggca ggacgcgccg aaacgccgtg ttgagccagt ccacgatgtc
      421 ggcctgcgaa cggaagttca ccgagagcgt caggggctcg agcgtcacgg cgccgatgcc
      481 ctggtgccgc gcgcgcagga acagcccgac gtcggcctcg cggaacctgt agatcgactg
      541 catcgggtcg ccgacgagga acagcgtgcg tccgtctccc ggctgccagc ccgaggtgag
      601 ccgctcgagc aactcgaact gcgtgaacga cgtgtcctgg aactcgtcga cgaggaggtg
      661 ctggatgcgg tagtcgagcg cgagcaggag gtcggtgggc gcctccgacg cgccgagcgc
      721 cgcgatcgcg ccggcggcga tctcgatgaa atcgacttgt ccgcgctcca ggaagacgag
      781 cttgagctgc gccgccgcca gccgcagcag ccgggagatc gcgcccagcg tctcccattg
      841 cgcgtcggta tagcgcgccg gcggcacggc gcgcagggca tgcagcgcgg cgcgcagctc
      901 gtcaagggcc tcgagcgccg gagggacggg cttgcgccag gtccccttct ggacgacgag
      961 atcttcgccg agcttccgcc acgcttccac cgcggcgtct cggccgagcc gcggcggcac
     1021 gtcctccggc cacagcgagc gcacgcgctc gacctgctcg tcacgcaccg cggcgagcgc
     1081 cgactccagg cgcgcgcggt cgggcgcgcc gtatttgcgc agccactgat cgcgccgcgc
     1141 gagcatcgac gcgagcagcg tctcggcctg ggcgaagtcg ttgtcgaggt gcgccatcag
     1201 cgcggcgacc tcggcgccgg catcggcgtc gtcgatcatc gcgagcgtcg ctcgagccgc
     1261 ctcgcggtag tgcgcggtcg cgtcctccac gacgccgggc gtggtgccgg cgccgccgag
     1321 gagcggcatc tgccgggtga gcgacaggca cagggcgtcc atcgtctgga tgcgcatgcg
     1381 gcccggctgc tcctcgaggc gccagcccag cctcgcgtcg cgctcgagca cggcggctgc
     1441 gctctcccag gtcagggccc ggtgcggctc gggcgggcgc ggctcgcgtc tcgccctcgc
     1501 gagcgcctcc agcacgcgtc cgcgcatctc ggcggcagcc ttcttcgtga acgtgatcgc
     1561 ggcgatctcc tcgggctgcc gaacgcgcgc gagcagcacg agatagcgct ggatcaggag
     1621 ctcggtcttg cccgagcccg cgggcgcctg cacgatgaag gagccgtcgg gcgcgagcgc
     1681 gcgccgtcgc tgcgcgatat cggcgatggc gtcgggctcg ctcacgagag ctcgcgctcg
     1741 ttgatgcggc acagcgcgcg gcgcccgcat tgctcacagg tgtcgtcgcc gtgcttcggc
     1801 gcgacgcgcg catcgccggc cgcgaactcc cgaccgagcg catcgagctc cgcgcgccag
     1861 cgccggagca gctcgtccca cgagcggtag tcttccgcgg cctttacgcg ctggcgagtc
     1921 acctcgcaga cgcccggaat cggctcgccc gcgctcgaga tgccggcgaa gcgcatcgcg
     1981 ccagccttca cgcacgcgaa ggcgaccgcg gcgatcggct cgtcgccgcc gagcgcgtag
     2041 agcggcagct gcggctcgtc ggggcgcggc cccagccagc ccgagacgct caccggcccg
     2101 gtcttgtagt cgaggatggc gcggccgccc ggtgcgccct gcaaccggtc catgcgatcg
     2161 agcttgacgt tcaccgtcac gccgccgaag gtgacggcgc gcttctgctc ccgggccacg
     2221 acgtcgaagg gctcgcgctg cttctccttc tccagccact cgcgcccgag gcggacgagg
     2281 cgcgcgcgct cgacccgggc ggtccgcccg tcgatcgacg cctcgcccgc gtaacgctgc
     2341 ttgttgatcg cgtagtcggc cgccgcggcg agccgctcct cgagatcgcg cccggacgcg
     2401 gcatcgagct tcgccttcga gtcgagctca cccatcagct tctccagcat cgcgtgcacg
     2461 aggcgcccgc ggtccgacgc gtccggcccg gagcacagcg gctcgagggc gtccgcgcgc
     2521 agccggtgcg cggcataggc gcggaacggg cacgcggcct gatcgcggaa caccccggtg
     2581 ccgcccgcca ccacggccgt gcccacgagc gccggcgcgt gcgcgtcctc gatgcgctcc
     2641 atgcggcgcg cggcgcggat cgcctgcgtc gcggtcgccg actgcggcag ctgcagatcc
     2701 tccggcgcga ctgccggcac gtcgcggatg agggggctgg gaagaaggtc gcggtcctcc
     2761 tcgcgctgcg cgtgcgtgac gatcacctcc ggcgcggcgg tgagccatgc gcgcgtcagc
     2821 cggacgtcga gctcgagcgc cgcggcgggc gacgcctgcg cgatgcccgc ggcgcgctgc
     2881 agcacgatcg ggagcagtgg attggggctg gagcgcagcg gccacgcctc gtcggtgagg
     2941 ccggcgaccc agagatgatc gaactccagt cccgccgact cgagcacgcc gaggatctgc
     3001 accggaacgt ccggcgtctg cggctggaac agcacgccct cgatcatcgt ctggaaacgc
     3061 gcgagcacct cgccgaagcg cacgcggccg gcgacgcggt cgagcgaagc cagctcggtg
     3121 atcgcctggt gcagcttgtc gagcgcctgg tgctcggccg acgagagcgg ccgctcgccc
     3181 gggaacccga gcgctcccag cacgcgcagc agcgcgcgag cccattcgcc gggtcgcttg
     3241 gtgcccgtga gctcggcttt agcgacctcg gcaaggtccg aaaggcagcg cgtcaggacc
     3301 gggcattcgg cgacgcgcca gcggttgtcg gttgcggtgg cccgttcgat caccttgcgc
     3361 aaggcgtcga cgcccaccgt gcgcctcagt ctgcggcgca gctcggcgtc gaggcgcgcg
     3421 cgcgcgtcga gctccgtctc cgcgccggcg atgaacggcg agcgcaccag gcggctcgcg
     3481 cgctcgagct cgatgcgcgc gcccgaggcg accccgcgcg ccatgtcgaa cgcgatcatc
     3541 gcgaccgcca ccagcggata cgcggaaagc ggctcgccga gcgagatgtt gaagggcggc
     3601 gccggcgtct cgtcggacga ggcgatcgtg ccgccgggcg ccagggtctc ggagaagatg
     3661 cgccgcacgg cctcgcgcgc gcgggcgagg tcgggaacga ccacgccgat gcgcacgtgc
     3721 gcggcacctg cgcgggcggc cgactcgagg cgttgccgcg cccagcgtgc ggcggcggcg
     3781 atctcgtcgg tcgccgactc gaacgccagg cgccggctcg tccccggccg ggcctccgcg
     3841 gccgacatct cgacgcgcgc tccggccgcc gccagcgcgc cgaggaagtc gcgtgcctgc
     3901 ggcgtcagga tgtcgaaccc gtaggcgacc acgtccgatt gccgatcgag attcgacgcc
     3961 accaggtccg gcagacgcgc ccggtcgacc agccggtcac gcgcggtgag gccttcatag
     4021 cgccacgccc attcggcgaa ggcgcgtgca tcatcgcttc ccgggaattc gcgcagccga
     4081 tgcgacagcc tccacgcgtg cgcgagctcc cacgcttcgc gcgcgagcgc cgcggcgggc
     4141 gcggcggcga gcagcgagcg gccggccgcg gtgtcgccga tcaccgtctc ccacatgagc
     4201 tgctcctgcg gcccgctcag cagcgccggc agcgcggtgg cgcgatcgga gtaaagcagg
     4261 tcctcgtaca ggcgctcgac cagcgctggc agcggcagga tatcggccgt ctcccacgag
     4321 gcgaggccgc gcccggactg ccgcaggtcg taatcccgct tgagcgcggc cgcgaggcgc
     4381 gcgttcggcg tgaccacggt gggtcccgcc gagccggcct ccatcagccg ccgcagcagc
     4441 gcctcgcgcg atatctcggg aaagggggtc tcgccgttca tgccgcagtc cgccccgcgc
     4501 ggagcgcggg gcgcagcaaa agaccctatt gtgccgctca ggacgcggca gcgtcctgct
     4561 tcggctcgtc ccttgcagga atgaagaaga tgaacaggtt ggcgaccacg atggtcgcgg
     4621 cgaggaagta gaacgcggcc atgatcccgt agcggtccgc gatcacgccg ccgaggaccg
     4681 gaccgagcgc ggagccgatg tgctgcgccc cgaacagcac gccgatgctg gtcccgccca
     4741 tcttcttggg cgtggtctcc aggatccagg cctgcagcac cggccgcacc gcgtagagga
     4801 agaagccgag caccgcgatg aagaagatga aggcgctcga gcggccggcg atcgccatga
     4861 acagcagcac gacacccgtc atgcccatgc tgctcatcag gatgctgcga cggcccatgc
     4921 gatcggacag atggcccgcg atcggcgacg cggcgaaccc ggccgcctgc agcatgaaca
     4981 tgcacgcccc cacccagagg ggcgagtagt gcagctcgta cgcgagaaac aagggaagga
     5041 aggtcagcag cccggtctgc gtcatggtgc ggaacgacga gctcaccgtg agcaggatca
     5101 tcttgcggtt cttcagcagc tcgcgcagtc cctggaaata ctcacccagg gtctggccgt
     5161 ccctggggtg ctttctctcc gacgcttcgc cggcgaggcg cagcgagccc aggtagacga
     5221 ggatcaggcc ggcgatcacc acgcccggcg cgacgttgac gaagaccacc tggcgccagg
     5281 tgagcgtcgc gagcagcgcg ccgatcacga gcggcgcgac cgcgtcgccg acgttgccgc
     5341 ccatgccgtg gatcgagagc accagtccct tgcgcgcggg gaagcggctc gcgagcgtcg
     5401 ggatcgcggt cggatgccac aggttgttgc cgatgccgac gagcgaaata cagaccaaca
     5461 gcagccagta ggtatgcgtg aagcccatca gcaggtacgg gaagccgacc cagaagagcg
     5521 agagcgcgag caggaagccc ttgcgcccca ccgtgtcgac gaacatgccg cccgggatgt
     5581 tcgacaccgc cgccgcgatg ctctggcacg tcatgatgag gccgatctgg ctgtacgaga
     5641 ggccgagctc cttgccgatc agcggcagca gcaggtagaa ggtcgcgggg taccagtgcg
     5701 tcagggtatg gcccatcgag atcagccaga cttccctgaa cgaccgggtc ggggcggcgg
     5761 aggcggtgag gattgcgctc atcggttgcg gctcgctcag gtttcgtgat ggtgatgatg
     5821 cccgtgatcg tggtcatgat gatgaccgtg cgggtgtccg tgcggcagac gcgggtcggc
     5881 cttgaacacc agcgacttga ccgtgaccgg gacggtgttc gacggcagcc ggatgcggcc
     5941 gagatcgacg tagtcgaaat cccacagcgt gaccccgtcg atcaccagga tctcgctcgc
     6001 gacgcgcatc tcgtcgcgca ggatatggcc gagcgtctgc gcgatgtcgc cgtcgagcac
     6061 gatgtagagc ggcctggcct ctgcgatggt cgcgggcagc gcggcgacga gcgcccgcgc
     6121 gaacgcggcc acgcgctcgt acagcggcgg accctgccag cggaacgcga gcgcgacctc
     6181 ggtctcgccc tcggtcaggt cgaaggcggt gaagtgcccg cggacggcct tgacgagcgc
     6241 gcgctcgtcg atctcggcgc cgaaccggaa cggcggctgc agcacctgca ggttgcgccg
     6301 cggcagcagc gccgcgtggt cggagatgaa gatcgtgttg cccgacagct gcacgctgta
     6361 ctcggaggcg ccgagcgccg tcgcgcggat gcattcgccc ggcggcagca gcggccaggg
     6421 gagcgcgccc gagtcgagcc gccggcgcag cgcctggccg agcaggcgtc cgaggtcgcc
     6481 gaagtcgcgc gtctcgcggc cgtacacgta ttcggcgacg ccgcccgaga acatcacgcc
     6541 gtcgacgccg tcctcgggcc agggcagcgg ctcggtgagc cacaggctcg cgagcgcggg
     6601 ccgcgcggac cggggcgcga gcgctgagac cagcgtgtcg gccagcgatt cggcgacgcg
     6661 cgcccggtcc tcgcgcgacg ccgtatcgcc gaggcgccag tcgaagcccg cgtccgccgc
     6721 gtggcggcgc ccggtgggat cgagacgcac gatgacgccg cgctcgtcga ccaccgtgag
     6781 gcgtccgccg acgtgaatcg ccgcggtcgc ggtgacgcgc ccgcgctcga ccagcgcgag
     6841 cttggtcgtg ccgccgccga tgtcgacgtt caggatcctg cgctcgccgt cgtgcgacgc
     6901 ggcggcggcg cccgagccgt aggcggcgag catcgcctcc atgtggtggc cggcggtcgc
     6961 gcacacgaac tcgccgccct gctcggcgag cagtcccgcg atcgcctccg cgttctcgcg
     7021 ccgcaacgcc tcgccggtga ggatgacgac gccggcgtcg atgttgtcgg ggtgcagctt
     7081 cgcgtcctcg taggcgcggt cgatgatgtc gccgagcttc gcctcgtcga tcagggtctc
     7141 gctctgatag ggcgtgagcg acaccggcga ctggtacaag gtctcgcgcg agacgacgaa
     7201 ataacgactg gtgagatcct cggcgagccg gcgcagattg agccgcgaga acacgacctg
     7261 cgtgcccgag gagccgatgt ccatgccgac cgagaggagc gacacgttgt cctgcatcca
     7321 caggggatcg gggccgggcg agtccgggcc gaagtcgtcg tggtcgtgat cgtggtcgaa
     7381 gggctcgtcg ccgtcgtgcg tgtggccgta atcctcgccg tagacgtggt cggcgagcgt
     7441 gtgcgtgcgc ttgtcctgcg gcatgcccgc tagtttacct tgggagcggc cggcggaccg
     7501 ccgttccgtc tggtggaacg cgcgcttttt atcgttcggc tctcgaccgc ggtcagcgct
     7561 cgagcaggtt cttcttgtcc ttcaccttcg cccactcgtc ggcgtcgccc ggcgcctcct
     7621 tgcgctcgac gatcggcttc cagatcttgg cgagctcggc gttggtcgcg atgaaggcgc
     7681 gctgctcggg cgggacgtcg tcctcggcga agatcgcctc gaccgggcac tcgggcacgc
     7741 acagcgtgca gtcgatgcac tcgtccggat cgatcaccag catgttcggt ccttcgcgga
     7801 agcagtccac cgggcagacg tcgacgcagt cggtgtactt gcacttgatg cagctttcgg
     7861 tgaccacgta cgccatgggg tcgggacctc gctcgaggaa gctcgcagga cgtccggcgg
     7921 agccgggcga cggagcgcgg attctagcac gcgcgtcgcg tcgaagcctc tcttttgcca
     7981 gcgaaaataa cccgttaggt cctatgggca tgctttcgcg agcgggcgaa ttgggctagc
     8041 atactgcttg aactcgtcgg gcaccgcccc gatatccatt ccgtgccagc ggcgctgccg
     8101 cgtacccatt ccgagggaag actccatgag cgacctgatc caccacgtga gcgacaacac
     8161 gttcgagccc gaagtgctga agtccgacgt cccggtcctg gtggactact gggcggagtg
     8221 gtgcggcccg tgcaaggcga tcgcgccggt gctggacgag gtcgcgaagg aatactcggg
     8281 caagctgaaa gtggcgaagg tgaacgtcga cgagaaccag gagatcccgc gcaagtacgg
     8341 catccgcggc atcccgacgc tgatgctgtt caagaacggc aacgtcgagg cgcagaaggt
     8401 gggcgcgctt tccaagtcgc agctcaccgc gttccttgac agcaacatct aactcttcta
     8461 ttctcgccgc aaaaggccgg tggcggagat agccgccggc cctccggtgc ccgggaccgt
     8521 ttcttcgtaa ctgagggtac cgggcgcaat cctccccgtt tcggttccct gttttcctct
     8581 gcgaacggtc tgagggcgta catcccacgc gagcgttgtt tatgcattta tccgagctga
     8641 agagcctgca cgtcagcgcg ctgctggaca tggcgacgca gaacgaaatc gacggcgcga
     8701 accgcatgcg caagcaggag ctcatcttcg cgctcctgaa gaaccgcgcc aagaagggcg
     8761 aatccatctt cggcgacggc accctcgagg tgctgcccga cggcttcggt ttcctgcgct
     8821 cgcccgacac ctcgtacctc gcatcgaccg acgacatcta cgtgtcgccg tcgcagatcc
     8881 gccgcttcaa cctgcacacc ggcgactcga tcgaaggcga gatcaggacg cccaaggacg
     8941 gcgagcgcta tttcgcgctg gtcaaggtcg acaaggtcaa cgccgactcg cccgaggcgt
     9001 ccaagcacaa gatcctgttc gagaacctga cgccgctgca ccccgacgag cagctcaagc
     9061 tcgagcgcga catcaaggcc gaggagaaca tcaccagccg catcatcgac atcatcgccc
     9121 cgatcggcaa gggccagcgc gggcttatcg tctcgccacc gaaggcgggc aagaccgtgc
     9181 tcatgcagca gctcgcgcac gcgatcaccg ccaacaatcc cgacgcgacg ctgatcgtgc
     9241 tgctcatcga cgagcgtccc gaggaagtga cagagatgac gcgcacggtg cgcggcgagg
     9301 tggtcgcctc gaccttcgac gagccggcgt cgcgtcacgt gcaggtcgcc gagatggtga
     9361 tcgagaaggc gaagcgcctg gtcgagcaca agaaggacgt gatcatcctg ctcgattcga
     9421 tcacgcgcct ggcgcgcgcc tacaacacgg tcgtccccgc ctccggcaag gtgctgacgg
     9481 gcggcgtcga cgcgaacgcg ctgcagcggc cgaagcgctt cttcggcgcc gcgcgcaaca
     9541 tcgaggaagg cggctcgctc acgatcatcg ccaccgcgct catcgatacc ggcagccgga
     9601 tggacgacgt gatcttcgag gagttcaagg gcaccggcaa catggagctg cacctcgacc
     9661 gccggatgta cgagaagcgc atcttcccgg cgatcaacgt caaccgctcg ggcacgcgcc
     9721 gcgaggagct gctgatcgag gcgccgatcc tgcagaaggt ctgggtgctc cgcaagctcc
     9781 tctacccgat ggacgacctc gaggcggccg aattcctggt cgacaagatc aaggccacga
     9841 agtcgaacgg cgacttcttc gactcgatgc gccgcggctg accggcgcgt tgattcgcgg
     9901 ccgccgatct gcgaaaatta gcgattttcc acgggcttgc aacgcggccg cggggttccc
     9961 gcgcgaagcc ggcgagacca agcccacatg cgaggaaagc gcccatgaaa gcgaaagtcc
    10021 atcccgaata caaggaagtc caggtcacgt gcagctgcgg caacacgttc gtgacgcgct
    10081 cgacgctggg caaaccgctc cacgtcgagg tgtgctcggc gtgccacccg ttctacaccg
    10141 gcaagcagaa gatcgtcgac accgccggcc gggtcgagaa gttccgccag aagtacgccg
    10201 gcaagaagac gatcaaggcc aagcaggcgt ccgcgtaggt tcgtccgggc gttcgtccgc
    10261 gggattgccg cgatgccggg aaaagaggca gcttcggctg cctttttcgt ttggcggcgc
    10321 gtgaggctgc cgagcgcccc cgtcgtcctg ccgcttgcgc cggcgctcct ggtgctggtg
    10381 gcggccgcct atgtcctccc cggcgtgctg gcccacgacc cctggaagac cgaggatgcg
    10441 gtcggcatcg gcatcgtgca ccagatgctg gtgcacgggc agtggctcat cccgcatctc
    10501 gccggcgaac cgtacttcga cgacgggccg ctttactact ggctggccgc gctgtgcgcg
    10561 aagatcgcct cgccgatcct cgccgtgcac gacggcgcgc ggctggcgag cgcgctcgcc
    10621 atggcggcgt cgctcgcctg cgtctatcac acggggcgcg agctttacga ccgcgccgcc
    10681 ggcaccgcgg cggctcttgt cctgctcggg tcgctcggcg tgctcgtgca cgcgcacgag
    10741 acgctcgccg agatgggcat gctggccgca c
//
LOCUS       DATDMW010000342         9038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_100833, whole genome shotgun sequence.
ACCESSION   DATDMW010000342 DATDMW010000000
VERSION     DATDMW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100833"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1078)
                     /gene="hisC"
                     /locus_tag="VI319_14285"
     CDS             complement(2..1078)
                     /gene="hisC"
                     /locus_tag="VI319_14285"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY6865063.1"
                     /translation="MSAGPDKVIRDEVRAMSAYFVPTPAGMVKLDAMENPYGLPEPLR
                     REIAEALAGAELNRYPDPSAPALVARLRSAMAVPRECALLLGNGSDEIIHIMIEACAR
                     PGAVIMAPSPTFVMYSVYAKLCGLRYASVPLNADFALDADRLLAAMAEHRPAIVFLAY
                     PNPPAGNLFPDADVERLIAAAPGLVVLDEAYQPFARRSFMDRLAGFPNVVLLRTLSKL
                     GLAGIRLGYLVGPPQWIREFDKVRSPYNVNVLTQIVAEKALEHREVLDAQADAICAER
                     VRLTERLRALPGVLPYPSDANFVLARMPRALDVFEELKRRRVLVRCFPEHPLVKDCLR
                     LTVGTPAENDRLLDALRTTLAEFV"
     gene            complement(1075..2394)
                     /gene="hisD"
                     /locus_tag="VI319_14290"
     CDS             complement(1075..2394)
                     /gene="hisD"
                     /locus_tag="VI319_14290"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY6865064.1"
                     /translation="MPEIARLSTAAPDFEARLAALVAFDAALDEEAERAAATIVADVR
                     ARGDAAVLDHTARLDGVRAASVAELELPRAALERALAGLDREQREALEAAAARIRAYH
                     ERQLARSWEFAEADGTVLGQKVTAIDRVGVYVPGGKAAYPSSVLMNAIPAKVAGVREI
                     VMTVPAPRGERNPLVLAAAAIAGVDRAFAIGGAQAIAALAYGTSTVPAVDKIVGPGNA
                     YVAAAKRRVFGIVGIDMVAGPSEILVICDGSTDPDWIAMDMFSQAEHDESAQAILVSP
                     DERFIGRVVDAIDRLLPSMPRRDIIAASLARRGALIRSRDLEEACELANRIAPEHLEL
                     SVAEPGRWLDRLRNAGAIFLGPHSSEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQK
                     RSSIVGVSAAGARTLGPLAATLARGEGLHAHARSAEMRSETGAGKKP"
     gene            complement(2387..3037)
                     /gene="hisG"
                     /locus_tag="VI319_14295"
     CDS             complement(2387..3037)
                     /gene="hisG"
                     /locus_tag="VI319_14295"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY6865065.1"
                     /translation="MGPVITLALSKGRIFDETLPLLAAAGIRPLDDPDASRKLILRTT
                     RSDLRLLIVRASDTPTYVRHGAAELGVAGKDVLLEHGGEGLYQPLDLGIARCRMMVAV
                     PEGYDYAGAVRRGARLRVATKYMDTAREHFATKGVHVDLIRLYGSMELAPLVGLADAI
                     VDLVSTGGTLRANRLRAVEEIRPISARLIVNQAALKLKRDRIQPLLDAFARAVERA"
     gene            complement(3114..4367)
                     /gene="murA"
                     /locus_tag="VI319_14300"
     CDS             complement(3114..4367)
                     /gene="murA"
                     /locus_tag="VI319_14300"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY6865066.1"
                     /translation="MDKLVIEGGVPLSGAVRVSGAKNAALPLLCAALLTEQPVRLANI
                     PQLKDVSTMLRLLGQMGVQTSLDERLGVELSARSVTHPVAPYELVKTMRASILVLGPL
                     LARFGEARVSLPGGCAIGLRPVDQHIKGLEAMGAQISVEEGDIVARSGRLKGARIVMD
                     LVTVTGTENLMMAACLAEGTTVLENAAREPEVVDLARCLSAMGARVAGAGSDVITVEG
                     VARLSGTQHRIMPDRIETGTYLAAAAATGGRVRLLGADASLLDAVLEKLREAGAGIVA
                     DADTISLEMRGRPKSTSVRTAPYPAFPTDMQAQFMALNAIAEGAAVITETIFENRFMH
                     ALEMRRLGADIEISGNTAIVRGVPRLQGATVMATDLRASASLVIAGLAADGRTTVDRI
                     YHLDRGYEYIEEKLAQLGARIRRAH"
     gene            complement(4360..4605)
                     /locus_tag="VI319_14305"
     CDS             complement(4360..4605)
                     /locus_tag="VI319_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEY6865067.1"
                     /translation="MMVTPEEVQGYIESGLACREVRVAGDGAHFEALIVSEAFRGKNR
                     VQRHQMVYAALGERMREEIHALSMRTLTPEEAQESHG"
     gene            complement(4614..5381)
                     /locus_tag="VI319_14310"
     CDS             complement(4614..5381)
                     /locus_tag="VI319_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6865068.1"
                     /translation="MPTARSGFPTLLYKEVLRFWKVSVQTIAAPALTAVLYLLLFAHV
                     MENRLQVYGSIRYTAFLVPGLVMMSVLQNAFANSSSSLIQSKITGNIVFVLLPPLSYW
                     EFFAAYVLAAVVRGLIVGAAVIAVTVPFVHLPMLEPWWVVLFAAAGSALLGTLGVIAG
                     VWAEKIDQVAAFQNFLVMPLTLLSGVFYSIHSLPAFWQGLSHFNPFFYMIDGFRHGFF
                     GVSDVDPLRSLAVVAAALAVATTIALAMLKGGYKLRH"
     gene            complement(5381..6280)
                     /locus_tag="VI319_14315"
     CDS             complement(5381..6280)
                     /locus_tag="VI319_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6865069.1"
                     /translation="MTAAIQIDDVHKRFGPLQALAGIDLEVRRGEFFGLLGPNGAGKT
                     TLINILAGLVRPDRGGARVLGHDVRGAYRLARRALGVVPQELVFDPFFTVRETLEIQS
                     GYFGIRNNRAWIDEVMEHLDLTGKADANMRALSGGMKRRVLVAQALVHKPDVIVLDEP
                     TAGVDVELRQGLWQFIRGLNRDGHTVVLTTHYLEEAELLCQRIAMLKQGRVVALDSTA
                     NLLKQVHHLFLRVRVGGAPLPAALAPLEHGSASGAHVLRLEDYGDIEPVLAQLRAAGC
                     RIEELELVQPDLEDVFVELMKAD"
     gene            complement(6309..6593)
                     /locus_tag="VI319_14320"
     CDS             complement(6309..6593)
                     /locus_tag="VI319_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6865070.1"
                     /translation="MIRRNADRLVVEGPVTLDTVPELAAGLEAQLGTGDAVVDFGGVT
                     DVDSSAVALVLEWRRQAEVRGVSLRVANAPEALQNLARLYGVLELLPLAR"
     gene            complement(6590..7207)
                     /locus_tag="VI319_14325"
     CDS             complement(6590..7207)
                     /locus_tag="VI319_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865071.1"
                     /translation="MKKILSTLALIALASTALAQDATPDALVKSITHDVLAVLRQNAN
                     AQDPKQIANIIETKVLPHFELTRMTQLAMGRNWRAASPEQQKALTAEFMTLLVRTYST
                     ALASYRDQVIDVKPLRAAPNATDVTVKSDVKRPGSQPIAIDYDLGKTSAGWKVYDVKV
                     GGVSLVTTYRETFANEVRENGIDGLIKSLSTKNRQSDARFGASKT"
     gene            complement(7215..7931)
                     /locus_tag="VI319_14330"
     CDS             complement(7215..7931)
                     /locus_tag="VI319_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY6865072.1"
                     /translation="MAILARLAAVAALVTAVAGCASPGGNPADPLEPLNRAMFGFNEK
                     VDNAVLKPVAQGYRAVTPQMVRTGVTNFFSNLEDLWISANNLMQGKAEWALGDLLRFT
                     FNSSFGLLGVIDVASDMGLEKHNEDFGQTLGRWGVGSGAYLVLPLLGPSTVRDGAARV
                     VDWQGDVVSNLSNVPTRNTLYATRVVSDRSNLFDATSLLDEAALDKYTFVRDSWLQRR
                     RSLVYDGSPPREPSDSDEPK"
     gene            complement(7945..8424)
                     /gene="mlaD"
                     /locus_tag="VI319_14335"
     CDS             complement(7945..8424)
                     /gene="mlaD"
                     /locus_tag="VI319_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY6865073.1"
                     /translation="MTRKELDLWVGLFVVIGIGAMLFLSLKVANLASFSASDTYPIMA
                     KFDNIGGLKVRAPVKSAGVVVGRVGEIRFDNESFEAVVVLNLDSHYQFPRDTSAKILT
                     SGLLGEQYIGLTAGGDSANLKAGDALKITQSAVVLENLIGQFLYNKASEGGSGDGKK"
     gene            complement(8430..>9038)
                     /gene="mlaE"
                     /locus_tag="VI319_14340"
     CDS             complement(8430..>9038)
                     /gene="mlaE"
                     /locus_tag="VI319_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEY6865074.1"
                     /translation="LVSGLFVGMVLGLQGYDTLVRYGSSEALGVLVSLSLVRELGPVV
                     AGLLFASRAGSAITAEIGLMKATEQLSAMEMMAVDPIARVVAPRFWAGVISMPLLAAL
                     FSAMGVFGGYLVGVRLIGVDEGSFWSQMQAAVDFREDILNGIIKSLVFGVAVTWIAVF
                     EGYDAPPTAEGVSRATTRTVVTSSLAILALDFVLTALMFRGA"
BASE COUNT         1461 a         3124 c         3103 g         1350 t
ORIGIN      
        1 cctacacgaa ttcggcgagc gtcgtccgca gcgcatcgag cagccggtcg ttctcggccg
       61 gggtgccgac ggtgagacga agacagtcct tcaccagcgg atgctcgggg aagcagcgca
      121 ccaggacgcg ccggcgcttc agctcctcga agacgtcgag cgcgcgcggc atgcgcgcga
      181 gcacgaaatt cgcgtcgctc ggataaggca gtacacccgg aagcgcgcgc agacgctcgg
      241 tcaggcggac gcgctcggcg cagatcgcgt ccgcctgcgc gtcgagcacc tcgcggtgct
      301 cgagcgcctt ctcggcgacg atctgggtga gcacgttgac gttgtacggg ctgcgcacct
      361 tgtcgaactc gcggatccac tgcggcggcc cgacgagata gccgagccgg atcccggcga
      421 gcccgagctt cgagagggtc cgcagcagca ccacgttcgg aaacccggcc agccggtcca
      481 tgaagctgcg gcgcgcgaac ggctgatacg cctcgtcgag gaccaccagg ccgggcgccg
      541 cggcgatcag acgctcgacg tcggcgtcgg ggaacaggtt tcccgccggg ggattgggat
      601 aagcgagaaa cacgatcgcg ggacggtgct ccgccatcgc cgcgagtaag cggtcggcgt
      661 cgagcgcgaa gtcggcgttc agcggcacgg acgcatagcg cagaccgcag agcttggcgt
      721 agacgctgta catgacgaag gtcgggctcg gcgccatgat caccgcgccg ggccgcgcgc
      781 aagcctcgat catgatatgg atgatctcgt cggagccgtt gccgagcagc agcgcgcatt
      841 cgcgtggcac cgccatggcc gagcgcagcc gcgcgacgag cgccggcgcg gaagggtcgg
      901 ggtaacggtt cagctcggcg cccgcgagcg cctccgcgat ttcgcggcgc agcggctcgg
      961 gaaggccgta cgggttctcc atggcgtcga gcttcaccat gcccgccggc gtcgggacga
     1021 agtacgcgct catcgcgcgc acctcgtcgc ggatcacctt atcgggaccg gcgctcacgg
     1081 cttcttcccg gcgccagtct cggatcgcat ctccgcggac ctggcgtggg cgtgcagccc
     1141 ctcgcctcgg gcgagggtcg ccgccagtgg tccgagcgtg cgggcgccgg cggccgagac
     1201 gccgacaatg ctcgagcgct tctggaagtc gtagacgccc agcggcgacg agaagcgcgc
     1261 ggtgcgcgag gtcggcagca cgtggttcgg cccggcgcag tagtccccga gggcctcgct
     1321 cgagtgcggc ccgaggaaga tggcgcccgc gttgcgcagc cggtcgagcc aacgcccggg
     1381 ctccgccacg gagagctcga ggtgctcggg ggcgatgcgg ttggcgagct cgcacgcctc
     1441 ctcgagatcg cgcgagcgga tcagcgcgcc gcggcgcgcg agcgaggcgg cgatgatgtc
     1501 gcggcggggc atcgacggca gcagccggtc gatcgcgtcg accacgcgcc cgatgaagcg
     1561 ctcgtcgggc gacacgagga tcgcctgcgc cgactcgtcg tgctcggcct gcgagaacat
     1621 gtccatcgcg atccagtcgg ggtcggtgct tccgtcgcag atgaccagga tctccgaagg
     1681 gcccgcgacc atgtcgatgc cgacgatacc gaagacgcgg cgcttcgccg ccgcgacgta
     1741 ggcgttgccg ggaccgacga tcttgtcgac cgccggcacg gtggacgtgc cgtaggcgag
     1801 cgcggcgatc gcctgcgcgc cgccgatcgc gaacgcccgg tcgacgcccg cgatcgcggc
     1861 cgcggcgagg acgagcgggt tgcgctcgcc ccgcggcgcg gggacggtca tcacgatctc
     1921 gcgtacgccc gccactttcg ccgggatcgc gttcatgagc actgacgacg gatacgcggc
     1981 ctttccgccc gggacgtaga ccccgacgcg gtcgatcgca gtgaccttct ggcccagcac
     2041 ggtgccatcg gcctcggcga actcccacga gcgcgcgagc tggcgctcgt gataagcccg
     2101 gatgcgcgca gccgccgcct cgagcgcctc gcgctgctcg cgatcgagcc cggcgagcgc
     2161 gcgctcgagc gccgcgcgcg gcagctccag ctcggcgacg ctcgcggctc gcacgccgtc
     2221 cagccgtgcg gtgtgatcga gaaccgccgc atcgccgcgg gcgcggacgt cggcgacgat
     2281 cgtcgccgcg gcgcgctccg cctcctcgtc gagcgcggcg tcgaacgcga cgagcgcggc
     2341 cagtctcgcc tcgaagtccg gcgcggccgt cgaaaggcgc gcgatctcag gcacgctcga
     2401 ccgcccgcgc gaaggcgtcg agcagcggct ggatgcgatc gcgcttcagc ttgagcgccg
     2461 cctggttgac gatgaggcgc gccgagatcg gccggatctc ctcgaccgcg cgcagccggt
     2521 tggcccgcag cgtgccgccc gtcgatacca ggtcgacgat ggcgtcggcg agaccgacga
     2581 ggggcgcgag ctccatcgag ccgtagagcc tgatcagatc gacgtggacg cccttcgtgg
     2641 cgaagtgctc gcgagccgtg tccatgtact tcgtcgccac gcgcagccgc gcgccgcggc
     2701 gcacggcccc ggcgtaatcg tagccttcgg gaacggcgac catcatgcgg cagcgcgcga
     2761 tgccgaggtc gagcggctgg tacaggccct cgccgccgtg ctcgaggagc acgtccttgc
     2821 ccgcgacgcc gagctccgcg gcgccgtgcc gcacgtaggt gggcgtgtcc gaggcgcgca
     2881 cgatgagcag ccgcaggtcg ctgcgcgtgg tgcgcaggat cagcttgcgc gaggcgtcgg
     2941 gatcgtcgag cggacgtatg ccggcggccg cgagcagcgg cagcgtctcg tcgaagatgc
     3001 ggcccttgga gagggcgagg gtgatcaccg gaccgatatc gcgtcggata aaacgcgatt
     3061 ctaccgccaa agggtcggga ccgtcattaa aaacaaaggt ttgcggtgcc gagctagtgc
     3121 gcgcggcgaa tccgcgcgcc gagctgcgcg agcttctcct cgatgtactc gtagccgcgg
     3181 tcgaggtggt agatgcggtc caccgtcgtg cggccatcgg ccgcgaggcc ggcgatcacc
     3241 aggctcgccg aggcacgcag gtcggtggcc atgaccgtgg caccctggag gcgcggtacg
     3301 ccccggacga tggccgtgtt gcccgagatc tcgatatcgg cgccgaggcg gcgcatctcg
     3361 agcgcgtgca tgaagcggtt ctcgaagatg gtctcggtga tcaccgcggc gccttcggcg
     3421 atcgcgttga gcgccatgaa ctgcgcctgc atgtcggtcg gaaacgcggg atacggcgcg
     3481 gtgcgcacgc tggtgctctt cggccggccg cgcatctcga gcgagatcgt gtcggcgtcg
     3541 gcgacgatgc ccgcacccgc ctcgcgcaat ttctcgagga ccgcgtcgag caggctcgcg
     3601 tccgcgccga gcagccgcac gcgcccgcca gtcgccgcag cggccgccag gtaggtcccg
     3661 gtctcgatgc ggtcgggcat gatgcgatgc tgcgtccccg agagccgggc gacgccttcg
     3721 accgtgatga cgtcgctccc cgcgcccgcg acgcgcgcgc ccattgcgct caggcagcgc
     3781 gccagatcga cgacctcggg ctcgcgcgcg gcgttctcga gtaccgtcgt gccctcggcg
     3841 aggcaggccg ccatcatcag gttctcggtg ccggtgactg tgaccaggtc catgacgatg
     3901 cgcgcacctt tgaggcgtcc ggagcgcgcg acgatgtcgc cctcctccac cgagatctgc
     3961 gcgcccatgg cctcgaggcc cttgatgtgc tggtcgaccg gccgaaggcc gatcgcgcat
     4021 ccgccgggca gcgagacacg cgcttcgccg aagcgcgcga gcagcggccc gagcaccagg
     4081 atggacgcgc gcatggtctt caccagctcg tacggggcga cggggtgggt tacgctccga
     4141 gcgctcagct cgacgccgag ccgctcgtcg agcgaagtct gcacccccat ctggccaagc
     4201 agccgcagca tcgtgctgac gtccttcagc tgcgggatgt tggcgagccg caccggctgc
     4261 tcggtcagca gcgccgcgca cagcagcggc agcgcggcgt tcttggcgcc cgagacgcgc
     4321 accgcgcccg agagcggcac accgccctcg atcaccagct tatccatgcg attcctgcgc
     4381 ttcctcgggg gtcagcgtgc gcatcgacag cgcgtggatt tcctcgcgca tgcgctcgcc
     4441 gagcgcggcg tacaccatct ggtggcgctg cacgcggttc ttgccgcgga acgcctcgct
     4501 cacgatcagc gcctcgaaat gcgcgccgtc gcccgcgacc cggacttccc ggcacgcgag
     4561 cccggactcg atatatccct gcacttcctc gggtgtcacc atcatcggtt ccttcaatgc
     4621 cgcagcttgt agccgccttt caacatcgcg agcgcgatcg tggtcgcgac ggcgagcgcg
     4681 gcagccacca cggcgaggct gcgcaacgga tcgacgtccg acacgccgaa aaagccgtgc
     4741 cggaaaccgt caatcatgta gaaaaatggg ttgaaatgcg agagcccctg ccaaaacgcg
     4801 ggcagcgaat ggatcgagta gaagacgccc gagaggaggg tcagcggcat gaccaggaag
     4861 ttctggaacg ccgcgacctg atcgatcttc tccgcccaga cgcccgcaat cacaccgagc
     4921 gtcccgagca gggcgcttcc tgcggcagcg aagagcacga cccaccacgg ctccagcatc
     4981 ggcaggtgca cgaagggcac ggtcacggcg atcacggctg cgccgacgat caggccgcgc
     5041 accacggcgg cgagtacgta cgcggcgaag aattcccagt acgagagcgg cgggagcagc
     5101 acgaagacga tattgccggt gatcttcgac tggatgagcg acgaggagct gttcgcgaac
     5161 gcgttctgca gcacggacat catcaccagg ccgggcacga ggaacgcggt gtaccggatg
     5221 ctgccgtaga cctgcaggcg attctccatg acgtgcgcga acagcagcag gtatagcacg
     5281 gccgtcagcg ccggcgccgc gatcgtctgc acgctcactt tccagaagcg caggacctcc
     5341 ttgtagagga gggtcgggaa cccactgcgt gcggtcggca tcagtcggcc ttcatgagct
     5401 cgacgaacac atcctcgagg tccggctgga cgagctcgag ctcctcgatg cggcatccgg
     5461 ccgcgcgcag ctgcgcgagc acgggctcga tgtcgccgta gtcctcgagc cgcagcacgt
     5521 gcgcgcccga tgcgctgccg tgctcgagcg gcgccagcgc cgcgggcagc ggggcgccgc
     5581 ccacgcgcac gcgcaggaac aggtgatgca cctgcttcag caggttggcg gtcgagtcca
     5641 gcgcgacgac gcgaccctgt ttcagcatcg cgatgcgctg gcacaggagc tcggcctcct
     5701 cgagatagtg cgtggtcagc accacggtgt gcccgtcgcg gttgaggccg cggatgaact
     5761 gccataatcc ctggcgcagc tcgacgtcga cgcccgccgt cggctcgtcg agcacgatca
     5821 cgtcgggctt gtgcacgagc gcctgcgcga cgagaacgcg gcgtttcatg ccgccggaca
     5881 gcgcgcgcat gttggcgtcc gccttgccgg tgaggtccag gtgctccatc acctcgtcga
     5941 tccacgcgcg gttgttgcgg atgccgaaat acccggactg gatctcgagc gtctcgcgca
     6001 cggtgaagaa cggatcgaac acgagctcct gcggcaccac gccgagcgca cggcgcgcga
     6061 gacgataggc gccccgcacg tcgtggccga gcacgcgcgc gccgccgcga tccgggcgca
     6121 ccagaccggc gaggatattg atcagcgtgg tcttgccggc accgttcggc ccgagcaggc
     6181 cgaagaactc gccgcgcctc acctcgaggt cgatcccggc cagggcctgc agcggcccga
     6241 agcgcttgtg gacgtcgtcg atctgtatgg cggcagtcat gctcgcgccg gggctgggcg
     6301 agccggcttc agcgggcgag aggaagcagc tcgagcactc cgtacagccg ggcgagattt
     6361 tgcagcgcct cgggggcgtt cgcgactcgc agcgacacgc cgcggacctc ggcctgccgc
     6421 cgccactcga gcacgagcgc gaccgcggag gagtccacat cggtcacgcc gccgaagtcc
     6481 acgaccgcat caccggtgcc gagctgcgcc tccagcccgg cggcgagctc gggcacggtg
     6541 tcgagagtca ccgggccttc gacaaccagc cggtcggcgt tgcgccggat cacgtcttgc
     6601 tcgctccgaa tcgggcgtcg ctctgccggt tcttcgtgga gagcgacttg atcaggccgt
     6661 cgatcccgtt ctcgcgcacc tcgttcgcga acgtctcgcg gtacgtcgtc accaggctca
     6721 cgccgcccac cttgacgtcg tagaccttcc agccggccga tgtttttccc agatcgtagt
     6781 cgatggcgat cggctgggat ccgggccgct tcacgtcgga cttgactgtg acgtcggtcg
     6841 cgttcggcgc ggcgcgcagc ggcttcacgt cgatcacctg gtcgcggtag ctcgcgagcg
     6901 cggtcgagta ggtgcgcacc agcagcgtca tgaactcggc agtgagcgct ttctgctgct
     6961 cgggcgacgc cgcgcgccag ttgcggccca tggcgagctg cgtcatgcgc gtgagctcga
     7021 agtgcggaag caccttcgtc tcgatgatgt tcgcgatctg cttcggatcc tgcgcgttcg
     7081 cgttctgcct cagaaccgcg agcacgtcat gcgtgatcga cttgaccagg gcgtcgggcg
     7141 tcgcgtcctg tgccagcgcc gtcgacgcga gggcgatcag ggcgagcgtg ctcaggatct
     7201 tcttcatgat ggcctcactt cggctcgtcg gaatcggaag gctcgcgcgg cgggctgccg
     7261 tcgtacacca ggctgcggcg gcgctgcagc caggaatcgc gcacgaacgt gtatttgtcg
     7321 agcgccgcct cgtcgagaag gctggtcgcg tcgaacaggt tggagcggtc actcaccacg
     7381 cgcgtcgcgt agagggtgtt gcgggtcggt acgttgctca ggttgctcac aacgtctccc
     7441 tgccagtcga ccacgcgtgc ggcgccgtca cgcaccgtag aaggaccgag cagcggcagc
     7501 acgagatagg cgccgctgcc gacgccccag cggccgagcg tctgcccgaa atcctcgttg
     7561 tgcttttcca gtcccatgtc ggaggcgacg tcgatcacgc cgagcagacc gaagctcgag
     7621 ttaaacgtga accgcagcag gtcgcccagc gcccactccg ccttcccctg catcaggttg
     7681 ttggccgaga tccagagatc ctcgaggttg gagaagaagt tggtgacgcc ggtgcgcacc
     7741 atctgcggcg tcacggcgcg gtatccctgc gcgacgggct tcagcacggc attgtcgact
     7801 ttctcgttga agccgaacat cgcccggttg agcggctcca gcgggtcggc gggattgccg
     7861 ccggggctcg cgcagcctgc gaccgcggtc acgagcgccg cgacggcggc tagccttgcg
     7921 agtatcgcca ttcgccctgc attcctactt cttgccgtct ccgctgccgc cctccgacgc
     7981 cttattgtac aagaactggc cgatgagatt ttccagcacc acggcggact gggtgatttt
     8041 cagcgcgtcg ccggccttca agttggccga atcgcccccc gcggtgaggc cgatgtactg
     8101 ctcgccgagc agtcccgacg tgaggatctt cgccgaggtg tcgcgcggga actgatagtg
     8161 gctgtccaga ttgagcacga ccaccgcctc gaacgactcg ttgtcgaaac ggatctcccc
     8221 gacgcggccg accacgacgc cggcgctctt caccggcgcg cgcaccttca gcccgccgat
     8281 gttgtcgaac ttggccatga tcgggtaggt gtcggaggcg gagaacgagg cgaggttcgc
     8341 gaccttgagc gacaggaaca gcatcgcccc gatgccgatc accacgaaca gccccaccca
     8401 gagatcgagt tccttgcggg tcatcatgat caagctcccc tgaacatcag cgcagtcaat
     8461 acgaaatcga gggcgagaat cgcgagcgac gacgtgacca ccgtgcgcgt cgtcgcgcgc
     8521 gacacgcctt cggccgtcgg cggcgcgtcg tagccctcga acaccgcgat ccacgtcacc
     8581 gccacgccga acaccaggct cttgataatt ccgttcagga tgtcctcgcg gaagtccacc
     8641 gccgcctgca tctgcgacca gaaagatccc tcgtcgacgc cgatcaggcg cacgccgacg
     8701 agatagccgc cgaaaacgcc catcgcggag aacagcgccg ccagcaacgg catggagatg
     8761 acgcccgccc agaagcgcgg tgcgaccacg cgcgcgatcg gatcgacggc catcatctcc
     8821 atcgccgaaa gctgttcggt cgctttcatc agtccgatct cggccgtgat cgcgctgccc
     8881 gcgcggcttg caaacagcag cccggcgacg accggcccga gctcgcgcac gagcgagagc
     8941 gagaccagca cgccgagcgc ctcggacgac ccgtagcgca cgagggtgtc atagccctgc
     9001 agcccgagca ccatgcccac gaacagcccc gacaccag
//
LOCUS       DATDMW010000343         2579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2342275, whole genome shotgun sequence.
ACCESSION   DATDMW010000343 DATDMW010000000
VERSION     DATDMW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342275"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VI319_14345"
     CDS             <1..724
                     /locus_tag="VI319_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6865075.1"
                     /translation="GTELASDLGVTVGDKLRLVKSTGEGATLTVTGLVDLGSKGANQR
                     IAYVALHTAQALLGLTGGASSIDVTVRDIYAAEDVARAITASTGVEADSWIRTNEQFF
                     MAISAQKVANTTIRVFVALAIALGIAAVLIVSVVQKSREIGILRAMGASRGQVLRVFL
                     IEGAVLGLVGSAFGSVIAIAAILAWGAFVTNPDGTPLFPIGYDPVLFLASAVIAMLTG
                     LASALLPAMRGARLDPVVAIRG"
     gene            717..1418
                     /locus_tag="VI319_14350"
     CDS             717..1418
                     /locus_tag="VI319_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6865076.1"
                     /translation="MADAVLRLEAIRKSYGVGTPAELEVLHGIDLVLEKGEFSALMGP
                     SGSGKSTLLNVVGLLDRPTSGRLLIGGEDTTRLDDRALTRLRGRSIGFVFQYHHLLPA
                     FTAVENVAMPMLLDRGRPDAAMRRRAEELLEIMGLTPWREHLASDMSGGQQQRVAVAR
                     ALAMSPSLVRADDPTGTLDTRSAQGVFDLMREVNRASGTTVLLVTHNHDLARQCGRII
                     ELVDGRIVADRRAGG"
     gene            complement(1419..1655)
                     /locus_tag="VI319_14355"
     CDS             complement(1419..1655)
                     /locus_tag="VI319_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865077.1"
                     /translation="MKLIVESHSGHRGEQEPRAFRMGERRLGVIEIVDRWIGPDYRYF
                     RVSADDGNLYVLRRDDATDEWTLGAFTRAGPPAG"
     gene            complement(1665..2252)
                     /locus_tag="VI319_14360"
     CDS             complement(1665..2252)
                     /locus_tag="VI319_14360"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY6865078.1"
                     /translation="MAKRIVIIQGHPDPRRERFGRALAAAYADGAAAAGHEVRAVDVA
                     AIDFPILRTREEWESGPLPGTLRGAQDAIRWADHLVLLFPLWAGTMPALLKAFLEQVF
                     RPGFAMAASKRDLLWEKRLAGKSARVVVTMGMPAFVFRWWFRAHALKGLERNILAFCG
                     IGPIRESLVGSVDSPDDAARQEWLARMRALGRKGE"
     gene            complement(2262..>2579)
                     /locus_tag="VI319_14365"
     CDS             complement(2262..>2579)
                     /locus_tag="VI319_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6865079.1"
                     /translation="HARENPSLFQTGWFVESVLSQTLIVHVLRTAGVPFVASRPGAAL
                     VAATVAVCVFAGWLPFSPFAPLFGFAPLPAAYGFALAAILACYLGLTQALKTALVRRF
                     GID"
BASE COUNT          384 a          919 c          917 g          359 t
ORIGIN      
        1 cggcaccgag ctcgcgagcg acctgggcgt cacggtcggc gacaagctgc gtctcgtgaa
       61 gagcaccgga gaaggcgcga cgctgacggt caccggtctg gtcgatctcg gcagcaaggg
      121 cgcgaaccag cgcatcgcct acgtcgcgct gcacaccgcg caggcgctgc tcgggctcac
      181 cggcggcgcc tcgagcatcg acgtgaccgt gcgcgacatc tacgcggccg aggacgtggc
      241 ccgggcgatc accgcctcga ccggcgtcga ggccgacagc tggatccgga cgaacgagca
      301 gttcttcatg gccatcagcg cgcagaaggt cgccaacacg acgatccgcg tcttcgtcgc
      361 gctcgcgatc gcgctcggta tcgccgcggt gctgatcgtc tcggtggtgc agaaatcgcg
      421 ggagatcggg atcctgcgag ccatgggcgc ctcgcgtggg caggttctgc gcgtgttcct
      481 gatcgaaggc gcggtcctcg ggctggtcgg ctcggcgttc ggctcggtga tcgcgatcgc
      541 cgcgatcctc gcctggggcg ccttcgtcac caacccggac ggaacgccgc tgttcccgat
      601 cggctacgac cccgtcctgt ttctcgcgtc ggccgtgatc gcgatgctga ccgggctcgc
      661 atcggcgctg ctgcccgcga tgcgcggcgc gcgcctggat ccggtggtgg cgatccgtgg
      721 ctgacgcggt cctcaggctc gaggccatcc gcaagtcgta cggcgtcggc acgccggcgg
      781 agctcgaggt gctgcacggg atcgacctcg tcctcgagaa gggcgagttc tccgcgctca
      841 tgggcccctc gggctccggc aagagcacgc tgctcaacgt cgtcggactg ctcgaccggc
      901 cgacgagcgg gcggcttctc atcggcggcg aggacacgac tcgcctcgac gaccgcgcgc
      961 tcacgcgcct gcgcgggcgc tcgatcggct tcgtgttcca gtatcaccat cttctgccgg
     1021 cgttcacggc ggtggagaac gtggccatgc cgatgctcct cgaccgcggc cggcccgatg
     1081 ccgccatgcg ccgccgcgcc gaggagctgc tcgagatcat gggcctcacg ccctggcgcg
     1141 agcacctcgc gtccgacatg tctggcgggc agcagcagcg cgtcgcggtc gcccgcgcgc
     1201 tcgcgatgag cccctcgctc gtgcgagccg acgatcccac cgggactctc gacacccgat
     1261 cggcgcaggg cgtgttcgac ctgatgcgcg aggtgaaccg cgcgagcggg accaccgtgc
     1321 tgctggtgac gcacaaccac gaccttgcgc gccagtgcgg gcgcatcatc gagctggtcg
     1381 acgggcgcat cgtggccgac cgccgcgcgg gcggctgatc agcccgcggg cgggccggcg
     1441 cgcgtgaagg cgccgagcgt ccactcgtcc gtcgcatcgt cgcgccggag gacgtacaga
     1501 ttgccgtcgt ccgcgctcac gcggaaatag cggtagtcgg ggccgatcca gcggtcgacg
     1561 atctcgatca ccccgagccg ccgctcgccc atgcggaagg cgcgcggctc ctgctccccg
     1621 cggtgtccgg aatgcgattc gacgatgagc ttcacccgcg caggttactc gcccttgcgc
     1681 ccgagcgccc gcatgcgcgc gagccattcc tgcctcgcgg cgtcgtccgg cgaatcgacc
     1741 gaacccacca gactctcccg gatggggccg atgccgcaga aagcgaggat gttgcgctcg
     1801 aggcccttga gcgcgtgcgc gcggaaccac catcggaaga cgaaggcggg catgcccatg
     1861 gtgacgacga cgcgcgccga cttgccggcg aggcgcttct cccacaggag gtcacgcttg
     1921 ctggccgcca ttgcgaaccc ggggcggaac acctgctcga ggaaggcctt cagcagcgcc
     1981 ggcatggtcc cggcccagag cgggaagagc agtaccaggt gatcggccca gcggatggca
     2041 tcctgcgccc cgcgcaacgt cccggggagc gggccggatt cccattcttc ccgcgtccgc
     2101 aggatcggaa agtcgatcgc cgcgacgtcg accgcgcgca cttcgtggcc ggccgccgcg
     2161 gcgccgtcgg cgtaggccgc ggcgagcgcg cggccgaagc gctcgcgccg gggatcgggg
     2221 tggccctgga tgatcacgat gcgcttggcc atggcgtctc ctcaatcgat gccgaagcgg
     2281 cggaccagcg ccgtcttgag cgcctgcgtc aagccgagat agcacgccag gatcgccgcg
     2341 agcgcgaacc cgtacgcggc gggcagcggc gcgaacccga acagcggagc gaagggcgag
     2401 aacggcagcc atcccgcgaa cacgcagacg gcgacggtgg ccgcgacgag cgcggcgccc
     2461 gggcggctcg cgacgaaggg cacgcccgcg gtgcgcagca cgtgcacgat cagcgtctgc
     2521 gaaagcacgg actccacgaa ccagcccgtc tggaagagcg acgggttctc gcgcgcgtg
//
LOCUS       DATDMW010000344        25372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_101397, whole genome shotgun sequence.
ACCESSION   DATDMW010000344 DATDMW010000000
VERSION     DATDMW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25372
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101397"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VI319_14370"
     CDS             <1..868
                     /locus_tag="VI319_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY6865080.1"
                     /translation="EACAVDGGLVWPIGEALVRAGHVRSGYTLREVIDALANRAGARR
                     SAQGAIGAGAGVPLASVTLLAPVLDPPSIWAAASNYKAHAEEMRAKGGSTDRSEYTPD
                     DLMAEFFLKPTSSIIGPGGTVVLPKISKDVDFECELCAVVGATARKVSEERALEHVFG
                     YTLCWDFSQRDPWGRGRQNTRNIRKGFDTFTGLGPWIVTRDEIPDPQNLAIRVEQNGK
                     LEMSAHTGDMICGVREHIRFLSGVLTLRPGDLITTGTPAGVRKLRPGDRLKGSIERIG
                     EMEVDVRAELGG"
     gene            991..1191
                     /locus_tag="VI319_14375"
     CDS             991..1191
                     /locus_tag="VI319_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865081.1"
                     /translation="MKALASIVLASAALGLAGCVVAPVEPAPVYAYGPPPPGIVFVPG
                     RTYAWGGSHHYYYGGGHWRHWH"
     gene            1173..2039
                     /locus_tag="VI319_14380"
     CDS             1173..2039
                     /locus_tag="VI319_14380"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEY6865082.1"
                     /translation="MAALALSGALALGAPAPALAWGEPGHRMVGAIADRYLNARAARE
                     VGFLLARDRLADRTPSGRRTLADVAYWADEIKDYPWGKRRASWHYDNIPMCGDADPAR
                     YCRRGSCASAQLERQIAILGDRARHPRERNEALKWVVHLMGDIHQPLHAADHHDQGGN
                     RVEVAFFGTRDNAPYGTIRLHTIWDIHLVERVLADRGGERAVVDAPVSAAQKARWEQG
                     SIADWMRESHEIAASVVYPMLPGGFACSRRYDGVIAIGEAYASRAEPVVEEQIRKAGI
                     RLARVLNETLGR"
     gene            complement(2017..3534)
                     /locus_tag="VI319_14385"
     CDS             complement(2017..3534)
                     /locus_tag="VI319_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEY6865083.1"
                     /translation="MSEESGGRIFFSSVGRSLEVEDEIRLTSVGVDIGSSTSHLAFSH
                     LVLERLDNRYVVSKREVFHESDVLLTPFLDDTTIDSTALGRFIEEQYRLAGIEPAAID
                     TGALILTGVAVRRANARAIGELFAAQAGKFVAVSAGDALETTLAAFGSGAAARSIRES
                     ARVMNVDVGGGTSKIAVCESGELVEATAIDVGARVVAFGADGRVERIEEAGRRFAAEA
                     GVVLRAGAAPDAAALDRIAERMAERLFEAMSRPALAAETAALLRLEPLANARAPDVVT
                     FSGGVSEYIYGRETRAFGDLGPRLARAIRARVERWGPRFERPEQGIRATVIGASQYTI
                     QVSGSTIFVSPHEALPLRNVPVIAPALPLDPESLDTGAIAAAIGHALRRLDLENGERA
                     VALCYRWSGSATFARLDAFCRGAASGLARVLARGHPLVLIGEGDVGGLIGIHCREELR
                     VPGAIVSVDGIALKEFDFVDVGALLAASGAVPVVIKSLVFPPSAALGRASGPGSR"
     gene            complement(3536..4750)
                     /locus_tag="VI319_14390"
     CDS             complement(3536..4750)
                     /locus_tag="VI319_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6865084.1"
                     /translation="MPHDKDQTHWHEPTGNKRAGFGQFKKQPTPYDAFMESEGVPVFR
                     GIGIRRVQDLPLMPWKRKGGRGHYIQLHGTETKWGCYVVEVPPAGALNPEKHMYEEIF
                     LVVEGRGTTEVWLEGDVKKHVFEWQKGSMFSIPMNAWYRLVNATSGGALLLAGTTAPN
                     VLNMINNVDAVFNNPYVFRDRFSGADDFYKPKDDIEPDPVRGLAMRRTNFIPDIVNCE
                     LPLDNRRSPGWRRVEPFMTNNQFYFWIGQHEQGRYSKAHAHTSAAVLICVKGAGYTYT
                     WPERCGVSPWKDGHADQVKRVDYEPVGMVSAAPGGARWYHQHFAASKEPFRLTAWFGP
                     HNPGRDPGAPGEKAIDYTGMDIPEGGTAIPYWMEDPFLRREYEDTLKKNGVPCRMDPG
                     WYETPKQHAPAK"
     gene            4924..5892
                     /locus_tag="VI319_14395"
     CDS             4924..5892
                     /locus_tag="VI319_14395"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865085.1"
                     /translation="MRAILRAAVLLCCLGPALAHAQAPYPTRPIRFIAPFPPGGSSDV
                     LCRLLGQKLTERLAQPVTVENRPGAGGNIGHEFAAKQPPDGYTILISSSSTLATNPHL
                     YKHLGFDPIADFAPISMVASAGQVLVVNPSVQAKSVAELVALAKARPGQLNFGSGGKG
                     IQSHISAEMFKAATGIDIVHVPYKGTVQAVSDLVAGQVQMVFSDMVPAMPHIRAGRLR
                     PLAVTTEKRSPVLPEVPTMIEAGIPGYHAGVWWAVVSPHGTPRAIVARLNTELGRIVQ
                     LADVREKYDGLGVQTEHSTPEEVTARIKADTLEVAKVLAAAGVEPE"
     gene            5893..6942
                     /locus_tag="VI319_14400"
     CDS             5893..6942
                     /locus_tag="VI319_14400"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY6865086.1"
                     /translation="MRRAIRAAGALFAATLLASCTGLMRPAGLVAPDAKWEEVSHAGR
                     VYAEGVVAAKDGAVYVSDLTRTGLVREGNPRGTIYRYDPATGTTTRYLEPSGMANGLH
                     VDRGGDLIMAQDADGGGRAVVRRNLATGQTSLVANAYRGKRLNSPNDVTSDAQGRIYF
                     TDARYGEPEPAELPNAVYRSDHDGGRLVQLSTDILRPNGIEVSPDGKRLYVSASNTAR
                     LPPNPNGPAQDRFGITMGGVVVYDLDADGNLTNGRVFYKRDDLVADGMAMDSDGDLYV
                     AFHNANPRDPKGAIVALDPGGALLAELPLPPGALPTNLGFGRGADAGSLYMTSAVPWK
                     LYRLKTLRHGHYFEQ"
     gene            6978..8270
                     /locus_tag="VI319_14405"
     CDS             6978..8270
                     /locus_tag="VI319_14405"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6865087.1"
                     /translation="MSPTPFELDLKLQVGKYDIRKMLGKGATGTVYLAVDTFSGREVA
                     LKTIEPEVFRDPEFGTVHRTQFLAEASLAGKLKHPHIVGILDAVVQEDSGYLAMELVT
                     GGDLSQFTAPGKLLPVADVLQIGFKCCGALDYAHREGIVHRDLKPANIMVAKGTEPKI
                     ADFGAALLRKTQAIQTASMGSPSYMSPEQLENRPLTFHSDMYSLGVVLYELLSGRKPF
                     AADNIQSLVQKILRDDPPLPSALRAGVPKSVDAIVLRAMGKRPEHRYKTWAEFALDLS
                     SAVKLVLPGEAVADSEKYVALKRVEMLIGLSDAELWEIAGAARWMRVLARKVMLRENE
                     PGNSFFFLARGQVKVTRQGRLLNMVDEREFFGEMAYIWGGKAARHATVEAATDLLVAE
                     FEPEAVAKMSEGAQLHLTRALARNLADRLELANTRLGR"
     gene            8284..9312
                     /locus_tag="VI319_14410"
     CDS             8284..9312
                     /locus_tag="VI319_14410"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY6865088.1"
                     /translation="MDGIDSRRRRFLAAGAALAAAGCAQQPALRPTRWTARQFHNQPA
                     LSHQHQFLTDLWLQVYKETDGRLNVTVYPQNDHIEGSDPAALDMLQSGELEFFTVMGS
                     ILGRKVPAAEIQSLPFAFTSHAQVHRVNDGALGEYLGRECAAKGIYRFQDGLLENGFR
                     HISMIERPINAAEDLRGVRMRVPDGRMFRDLFAALEAEPVTVNIRDLYDALKTRQVDG
                     QENPLVVTEVNKLYEVTRYVSITNHMWSGFNLLANLGFWNGLPGDVQEIVQRNVRKVV
                     AAQREYTDGLNRELQGRLASERGMIFNTADTASFRAKLGRAFYGRWRDQFGSTAWSLL
                     ENEVGTLG"
     gene            9360..11015
                     /locus_tag="VI319_14415"
     CDS             9360..11015
                     /locus_tag="VI319_14415"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6865089.1"
                     /translation="MLLVLGGVAAVAVESYRVVDRELTESALERRGAVSFLAATVLSE
                     KFDRLLDVGVALATRVRFRQLIEAGRWDEAIRILTRVPRDFPFIDRITINDASGTLRA
                     DIPPAPEIRGQNFAHRDWYQSVERTGRPYVSRVYRRSAPPERNVFVAAIPIKSDSGAT
                     IAVLVLQVRSDLFFEWTRGVEVGERGVVYVVDQRGFLAYHPRRASQQELVDYSRVPAV
                     QRVLQGARGVDVVSDPETRERQVVAYQPIGAHGWGVVLAEPADAVFALRNDQLKWMVF
                     TYVLSIVSLGALAYLASSIALHRRQIDVANETHAELERRVRERTLELERTNRELESFS
                     YSVSHDLRAPVRAIEGFARILEEEAGGRLDAESRRYVALVRDNGRRMGVLIESLLEFS
                     RLGRRPLNVADTDMGELARDVFEELAGGLPKVPELVLGPMPRARCDRALVRQVWTNLL
                     SNAIKFSGKTDAPRVEAGGEAKDGENVYHVRDNGAGFDMRYYEKMFGVFQRLHGDQEF
                     HGTGVGLALVQRVVERHGGRVWAEGKVGEGATFWFSLPAAGKA"
     gene            complement(11012..11401)
                     /locus_tag="VI319_14420"
     CDS             complement(11012..11401)
                     /locus_tag="VI319_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865090.1"
                     /translation="MLAALSCPAWAACEASDGCPDTVPALMASAEPAAAAAVQIAQIP
                     AEVVPALAPGDAGTAPERVAATLSDARPGSLVMRPEPSRPVARSPANERRAEQRSAPG
                     SESSSVWLMLFAGLAFAGFVVAKRSRG"
     gene            complement(11610..12815)
                     /locus_tag="VI319_14425"
     CDS             complement(11610..12815)
                     /locus_tag="VI319_14425"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6865091.1"
                     /translation="MDSKRLPVVVAGAGPTGLMCALALAQQGIPVVVCESGRALARDL
                     RAGTFHPPTLELMAPYGITDRMLEVGIPVRHWQIRDRRGEFVAQFDLGLLAEDTPYPY
                     RLHLEQHRLTPIQLDLVRSAGADVRFDHAVTGVEPRGDRVLVHVEAAGKPETIEASWV
                     IGADGGRSSVRKALGIEFEGFTWPEQFLVASTTHDFSRYGFALNAYIADPVEWIALFK
                     MPNSGPPGLWRAVFPVPDQAEDVVLAPENVEDKLQRFVARSVPYEVRYKSTYRVHQRV
                     CKAFRAGRVLLAGDAAHINNPIGAFGLNSGIHDAVSLAEKLGKVWRGDADESLLDVYA
                     RQRRTVAIEQVDKMSIRNKRMLEEHDPRVQRERLAELVAIASNPERARRHMLETSMIA
                     SLRQAAQIA"
     gene            complement(12834..13802)
                     /locus_tag="VI319_14430"
     CDS             complement(12834..13802)
                     /locus_tag="VI319_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865092.1"
                     /translation="MHRAWLLAGLVVCGSAAGQPASQNFPSQPIKFIVPFTPGTGMDI
                     IARHVGPKLTGRLGQPVVVENRPGASGNIGAEAVAKAAPDGHTLMVGANTLVIASNLY
                     KNVPFDPLRDFSPVTLAATTTMVLVVNPATGIRSVRELVARAKAQPGKLTYGSPGVGT
                     PHHMGMELFKDLTGADLLHVPYKGSAGAVTDLVSGQINVMLLPIHQVLPYRDAGRLRL
                     LAAAGAKRHPSAPDLPTLGELGIKGAEAADIWYAFYAPAGVPAPVIAKLNRELRAILA
                     QPEAKAVLQKVGLDAASSTPEELFALMKRDYARWGEIIRRNHITAE"
     gene            complement(13979..14761)
                     /locus_tag="VI319_14435"
     CDS             complement(13979..14761)
                     /locus_tag="VI319_14435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6865093.1"
                     /translation="MTAVAVGGARIDVEQIGSGPELLLLHSLLADRSAFDRVKGELAR
                     ERRVVLANMPGYGASSPAGASIEEYADHVAGLFPALKLSARTAVLGNGFGGFVALALA
                     ARHGAKFDRLIIADSAATFPPPAKEPFKVLHAKVSEQGMEAVLDAAVRRMFPEAFIAE
                     HPEVVAERKAALAEADPGCFARACLALARVDLREAVRAIRNPTLVMVGALDQTTPPGL
                     ARELAGAIPGARFLEIPGAGHCPQIEKPAEFVAAVQGFLAAP"
     gene            complement(14758..15999)
                     /locus_tag="VI319_14440"
     CDS             complement(14758..15999)
                     /locus_tag="VI319_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865094.1"
                     /translation="MASRRSLLFAFAALAVTALPVSSALAQGEPLRIGFLTVRTGPLA
                     AGGRQMEEGINLLLKERNNTFAGRKVEIYFADTAGQPAQAKSKTQELVERNHVHVIIG
                     PLATFEALAIDDYMLSSKTPLITPTSAAQMDLAQQGKSDYVVHVYGTAAQPMHVLGDF
                     AAKKLKLKKVAMVADDFTYGHEGAAGFHRVFEDDGGRIIQKLWPPLNVPDYGSYVGQI
                     KPDADGVYAGFAGSNPLRFLRAYREYGHTKPVFGNPTLVDEGILKNMSDEALGVYSAS
                     WYTVDRDTPDNKRFVESIQREYKVIPGFYTAGTYTAGLWLEQALKDIKGKFEDKAAFV
                     KALHSVKLDHGPMGPIRLDQYGKPVLNIYVRKVERKNGQLVNTTIETVREVSQFFRYD
                     PKQFLAQPTYSRDYPPAKNLE"
     gene            16109..17065
                     /locus_tag="VI319_14445"
     CDS             16109..17065
                     /locus_tag="VI319_14445"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY6865095.1"
                     /translation="MRPDLLALASSLAAREEGFALVTVVRREPPSSARVGDTALVTAT
                     GDYHGWAGGGCTRSTVVREALRAIADGEPRLLSLAPESSERHRPGLIALPMTCDSGGT
                     VEIYVEPVLPVPRLLLFGSTPAVRALAAIARAMGYRVEVVDPDAAPADFPGASVRSDL
                     SPGDIPRGAHVVVATMGDRDLEILDAALASGPAYLGVVASRKRFEPLRDALAAQGVSR
                     EALERVAAPAGLDLGARTPEEIALSIMAQIVERRRAGARPPREEPRAQPAREAVDPIC
                     GMSVTVAGARHTVEAGGTTWYFCCAGCRAKFLADPQRHARAI"
     gene            17223..18092
                     /locus_tag="VI319_14450"
     CDS             17223..18092
                     /locus_tag="VI319_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY6865096.1"
                     /translation="MIPASFDYHRPASLDEALKLLKKHGDDAKVLSGGMSLLPMMKLR
                     LASFSHLVDIGRIPGLDAIKEEKGVVRIGALARQAALERSEVVRAKLPILADALPLIA
                     DPLVRNRGTIGGNVANGDPGNDQPAIMIALGATFVARGSKERSIAAGDFYKGLYSTAL
                     APGEILTEVRIPVPPARSGGAYVKLKRKTGDFAVAASAVQLTLGKSGAIERCTIALTN
                     AGQTPVEAKEAGQFLAGKTPGDLTLREAAQIAAKASSPSADRRGSVEYKKEMARVLTA
                     RALKIALERAGRN"
     gene            18105..18581
                     /locus_tag="VI319_14455"
     CDS             18105..18581
                     /locus_tag="VI319_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6865097.1"
                     /translation="MATHKISVTVNGAAREAEVDSRLLLVHLIRENFRLTGTHVGCDT
                     SHCGACTVQLDGTPVKSCTVLAVQADGCKVTTVEGLEQGGKLHPVQEGFTEKHGLQCG
                     FCTPGMLMTTSALLAQNKNPSEQEIRQAISGNLCRCTGYVNIVKAVQYAAEKMRSA"
     gene            18595..20988
                     /locus_tag="VI319_14460"
     CDS             18595..20988
                     /locus_tag="VI319_14460"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEY6865098.1"
                     /translation="MSDSVNESQIKPRTDAKICGMGHSMKRKEDPRFIRGKGNYVDDI
                     QLPGMLYMDIVRSPHAHARIKKIDTSKALKIPGVLAVIDGPTLAKYNLHWMPTISADT
                     QMVLPIDEVMYQAQEVCAVIATERYIAADGVDAVEVEYEPLPVMVDPHKSLAPGAPVL
                     RKDKKDKKDNLAFHWEAGDKADAERALAACDVVVRENVYLPRIHVASMETCGCVADFN
                     SAEGKLTVYMTTQAPHAVRTVLALVAGHVGLSEERIRVVSPDIGGGFGGKVPVYPGYV
                     VAVAASVVIGKPVKWIEDRMENLQADSFARDYHMKVELGAKKDGTMTALKISTVADHG
                     YANASANPSKFPAGLFSICTGSYDLKHAFCEVDAAYTHKPPGGVAYRCSFRVTEAVHA
                     IERATDALAQKLGMDPADFRMKNFIRPEQFPYKSVLGWEYDSGDYPRALRKAMDIIGY
                     DALRREQAEKRKRGELMGIGICSFTEIVGAGPSKHFDILGLKMFDSAEIRIHPTGKAI
                     ARFGTKSQGQGHETTYAQIVAEELGIPAAHVQVEEGDTDTAPYGLGTYASRSTPTSGA
                     ACALAARKIRDKARNIAAHLLECSPQDLEWEPGKFYVKGAPQMAKTIQEIAFAAYTNH
                     PQGMEAGLEATHYYDPPNLTFPFGSYICVVDIDRGTGEVKVRRFVAVDDCGNIINPMI
                     VDGQIHGGLTQGIGPALYEEISYDEEGNISGGSFLDYYVPTALETPKWETDKTITPSP
                     HHPFGAKGVGESATVGAPPAIHNAVVDALAHLGVAHFDIPITPEKVWQTLRAKGVAE"
     gene            complement(20993..22462)
                     /locus_tag="VI319_14465"
     CDS             complement(20993..22462)
                     /locus_tag="VI319_14465"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6865099.1"
                     /translation="MTDNLAQALALLDTWAAHSAAQQRIPGVALAVVRGDRSVLTRAY
                     GFANLQARTPATPQTPYKIGSLSKSFTALAVLQLVEDGKLRLDDRFGDHVPAAAHAPV
                     PADVTVRQLLAHTSGLQRDLPGTMWSAPAFPAALPGDFSATFPPGTQWKYSNVGYALL
                     GELIAAVGGEPWARHIERRILAPLGMSGTQTTPRLDDPRIATGYSRPAPGADHAPARQ
                     ADHGAMRPAGGMVSTAEDMARYVAFHLGGAEGVLGARMLREMHRPQWLFEDWQTAWGL
                     GVQVRRADGRVRVGHPGSTPGFSALMEFIPALKLGVAVLTNADDGNPAAFCDYALQLL
                     APIVAKEIARTVPALPADAARYCGLYRSENRNLTMFVAMLDGQLTLVPPGAPNPHAAR
                     VILESTPEAHVFVMRSSGAFATVHFGERLEFSVAPGGEVTGYDMGSSARFVREPALIQ
                     TLYNGADRRGARSDRRQYPPPPYAGVERRSGLDRRLLMH"
     gene            22552..23274
                     /locus_tag="VI319_14470"
     CDS             22552..23274
                     /locus_tag="VI319_14470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY6865100.1"
                     /translation="MTFGRNAVMLAALPLFWLAMNAMAQTPAAPPPVPADVSPLYVIT
                     YVESRPSARNDAQALLKSYRAASRAAPGNLRSVVVRSVARPGQFVVASAWKDKAAWDA
                     HMAAPATQAFREKLAALRNAPADDRFHNSLTVGPLDNPGAARGAVYVVTHIDVIPPRK
                     DDAVAAIKAFGETARAASGNVRFEIVQQTNRPNHFTAFEVWRSRQAFDAHGMSAAMRE
                     FRDKLSGMQGALYDERLYELVD"
     gene            23450..23938
                     /locus_tag="VI319_14475"
     CDS             23450..23938
                     /locus_tag="VI319_14475"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6865101.1"
                     /translation="MWSSRPLYDGRYIAATPPLARSIRWDDVALLVARLTLAALFLPA
                     GVQKLLHMDQFVALVAQYDFFGYALPRPKFFAGLAVVVELGGGIMLLLGLFTRWAALA
                     LIAFLVVATAASHRYWEYRAPERQAQQIEFQSNIAIAAGLLALSVAGGGVMGLDRRRR
                     SY"
     gene            complement(23943..24821)
                     /locus_tag="VI319_14480"
     CDS             complement(23943..24821)
                     /locus_tag="VI319_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6865102.1"
                     /translation="MKLRTLLGMYPNTRALREGRIRPSECELDFADVKVPNRAFKAVV
                     RDLAFDVAELAIMTFLQAKAWGKPLVLVPARVGPARFQHQCLVHNTERGPLRPQDLAG
                     RRIGVRAYAQTTVTWVRGFLAEEHGVAPESARWITMEDGHLAEYRDPPQAERAPEGKE
                     LTAMLLAGEVDAAIIGSELPDDARVQPLIRDPEAAAKDWARRHRAVPINHMIVVKSAL
                     ARSAPEVVSEIYDVLQEARRAESPRTEGAPGRSEFNMAPYGVEANRSALEVAIDYATR
                     QGMLPRPLTVDELFEV"
     gene            complement(24818..>25372)
                     /locus_tag="VI319_14485"
     CDS             complement(24818..>25372)
                     /locus_tag="VI319_14485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6865103.1"
                     /translation="IRHWTGRRLRGLRVFIAGHTAAAKDARLDDPSAFPAWRYASESG
                     LPICVQMRGTYAQLDAVLTRFPNAKVLLDHMGRPSIADGEPYLDAAPLLELARHKNLF
                     LKLTTHNVRESRQGKATPETFFARVVKEFGASRIAWGSNYPASAGTLAALLADAREAL
                     ASLPKEYQEWIFSRTAQTLYPSLR"
BASE COUNT         3865 a         8852 c         8959 g         3696 t
ORIGIN      
        1 cgaggcgtgc gccgtcgacg gcggcctcgt ctggcccatc ggcgaggccc tggtgcgcgc
       61 cggccacgtg cgcagcggct acaccctgcg cgaggtgatc gacgcgctcg cgaaccgcgc
      121 cggcgcgagg cgcagcgcgc agggcgcgat cggcgccggc gcgggcgtgc cgctcgcctc
      181 ggtaacgctg ctcgcgccgg ttctcgatcc gccgtcgatc tgggccgcgg cgtccaacta
      241 caaggcgcac gccgaggaga tgcgcgccaa gggcggctcc accgaccgca gcgagtacac
      301 gcccgacgac ctgatggccg agttcttcct caagccgacc tcctcgatca tcggcccggg
      361 cgggacggtg gtcctgccga agatctcgaa ggacgtcgac ttcgagtgcg agctgtgcgc
      421 ggtcgtcggc gcgaccgcgc gcaaggtgag cgaggagcgc gcgctcgagc acgtcttcgg
      481 ctacaccttg tgctgggact tcagccagcg cgatccctgg ggacgcggcc gccagaacac
      541 gcgcaacatc cgcaagggct tcgacacgtt caccgggctc ggtccgtgga tcgtgacgcg
      601 cgacgagatt cccgaccccc agaacctcgc gatccgcgtc gagcagaacg gcaagctcga
      661 gatgagcgcg cacaccggcg acatgatctg cggcgtgcgc gagcacatcc gcttcctctc
      721 gggcgtgctg acgctgcgtc ccggcgatct gatcaccacc ggcacgccgg cgggcgtgcg
      781 caagctcagg cccggcgacc gcctcaaggg ctcgatcgag cgcatcggcg agatggaggt
      841 cgacgtgcgg gccgagctcg gcgggtgaga tgcgcgtgct tgtaacggcg cgtgacgcgc
      901 tcgctcatgc gaaagatgcg gtaacaattt ctacgggcct cggtggccgg atcgtgcgtt
      961 catacgggta tcgcaatccg gagatccctg atgaaagctc tcgcatcgat cgttctcgcc
     1021 tcggcggcgc tcggcctggc gggctgcgtc gtggcgccgg tggagccggc gccggtctac
     1081 gcgtacgggc cgccgccgcc gggaatcgtc ttcgtgcccg ggcgcacgta cgcctggggc
     1141 gggtctcacc actattacta cggcgggggg cattggcggc actggcactg agcggcgcgc
     1201 tcgcgctcgg cgcccccgcg cccgcgctcg cgtggggcga gcccggacac cgcatggtcg
     1261 gcgcgatcgc cgaccgctac ctgaacgcgc gcgcggcgcg cgaggtcggc ttcctcctcg
     1321 cgcgcgaccg gctcgcggac cgcacgcctt cgggacggcg caccctcgcc gacgtcgcct
     1381 actgggccga cgagatcaag gactatccct ggggcaagcg ccgcgcctcg tggcactacg
     1441 acaacatccc gatgtgcggc gacgccgatc ccgcgcgcta ttgccgccgc ggctcgtgcg
     1501 cgtcggcgca gctggagcgc cagatcgcga tcctcggcga ccgcgcgcgg catccgcgcg
     1561 agcgcaacga ggcgctcaag tgggtggtgc acctgatggg cgacatccac cagccgctgc
     1621 acgccgccga tcaccacgac cagggcggga accgtgtcga ggtcgccttc ttcggcacgc
     1681 gcgacaacgc gccgtacggc acgatccgcc tgcacacgat ctgggacatc cacctggtcg
     1741 agcgggtgct cgccgaccgc ggcggcgagc gtgccgtcgt cgacgcgccc gtgagcgcgg
     1801 cgcaaaaggc gcgatgggag cagggctcga tcgcggactg gatgcgcgag agccacgaga
     1861 tcgcagcgag cgtcgtctat ccgatgcttc cgggcggctt cgcgtgctcg cgccgatacg
     1921 acggcgtgat cgcgatcggc gaggcgtacg cctcgcgcgc cgagccggtc gtagaggagc
     1981 agatccgcaa ggcgggcatc cgcctcgcgc gagtgctcaa cgagaccctg ggccgctagc
     2041 acgcccgagc gcggccgacg gcgggaacac gagcgacttg atcaccacgg gcactgcgcc
     2101 cgaggccgcg agcagcgcgc cgacgtccac gaagtcgaac tccttgagcg cgattccgtc
     2161 gaccgacacg atcgcgcccg ggacgcgcag ctcctcgcgg caatggatgc cgatcaggcc
     2221 gccgacgtcg ccctcgccga tcagcacgag cggatggccg cgcgcgagca cgcgggcgag
     2281 gcccgaggcg gcgccgcggc agaacgcgtc gagccgcgca aacgtggccg acccgctcca
     2341 gcggtagcac agcgcgaccg cgcgctcgcc gttctcgaga tcgaggcgcc tgagcgcgtg
     2401 tccgatcgcg gccgcgatcg cgccggtgtc gagcgactcc gggtcgagcg gcagcgcagg
     2461 cgcgatcacc gggacgttgc gcagcggcag cgcctcgtgc ggcgagacga agatcgtgct
     2521 gccgctcacc tggatcgtgt actgggaggc gccgatcacc gtcgcgcgta ttccctgctc
     2581 ggggcgctcg aagcgcgggc cccagcgctc gacgcgcgcg cggatcgcgc gcgcgagcct
     2641 cgggccgagg tcgccgaagg cgcgcgtctc gcggccgtag atgtactccg acacgccgcc
     2701 cgagaaggtg acgacgtcgg gcgcgcgcgc gttcgcgagc ggctcgaggc gcagcagcgc
     2761 cgcggtctcg gcggcgagcg ctgggcggct catcgcctcg aacaggcgct cggccatgcg
     2821 ctcggcaatt cgatcgagcg ccgccgcatc cggagccgcg ccggcccgca gcacgacgcc
     2881 ggcctcggcg gcgaagcggc gcccggcctc ctcgatgcgc tcgacgcggc cgtctgcgcc
     2941 gaaggcgacg acgcgcgcgc cgacgtcgat cgcggtggcc tcgaccagct cgcccgactc
     3001 gcacaccgcg atcttcgagg tgccgccgcc gacgtcgacg ttcatcacgc gcgccgactc
     3061 tcggatcgag cgcgcggcgg cgcccgagcc gaaggcggcg agcgtcgtct cgagcgcatc
     3121 tcccgcgctc accgccacga acttgccggc ttgcgcggcg aacagctcgc cgatcgcgcg
     3181 cgcgttcgcg cgccgcacgg cgacgccggt caggatcagg gcgccggtgt cgatggcggc
     3241 gggctcgatg cccgcgagac ggtactgctc ctcgatgaac cgtccgagcg cggtcgagtc
     3301 aatcgtcgtg tcgtcgagaa acggtgtgag aagaacatcg gactcgtgga acacttcgcg
     3361 cttcgatacc acgtagcggt tgtcgagccg ctcgagcacc agatgcgaga aggcgaggtg
     3421 cgaggtcgag gagccgatgt cgaccccgac gctcgtcagc cggatctcgt cctcgacctc
     3481 gaggctgcgg ccgacgctcg agaagaagat gcggccgccg gactcctcgc tcacatcact
     3541 tcgccggagc gtgctgcttc ggagtctcgt accagcccgg atccatgcgg cacggcacgc
     3601 cgtttttctt cagcgtgtcc tcgtattccc ggcgcaggaa cgggtcctcc atccagtacg
     3661 ggatcgccgt gcctccctcg gggatgtcca tgccggtgta atcgatcgcc ttctcgcccg
     3721 gcgcgccggg atcgcggccg gggttgtgcg ggccgaacca cgcggtgagg cggaacggct
     3781 ccttcgacgc ggcgaagtgc tggtggtacc agcgcgcgcc gccgggcgcc gcgctcacca
     3841 tgccgacggg ctcgtaatcg acgcgcttca cctggtcggc gtggccgtcc ttccacggcg
     3901 agacgccgca gcgctcgggc cacgtgtacg tgtagcccgc gcccttgacg cagatcagca
     3961 ccgcggccga ggtgtgcgcg tgcgccttcg agtagcggcc ctgctcgtgc tggccgatcc
     4021 agaagtagaa ctggttgttc gtcatgaacg gctcgacgcg ccgccagccc ggcgagcgcc
     4081 ggttgtcgag cggcagctcg cagttgacga tgtcggggat gaagttcgtg cgccgcatcg
     4141 cgaggccgcg cacgggatcg ggctcgatgt cgtccttggg cttgtagaag tcgtcggcgc
     4201 ccgagaagcg gtcgcggaac acgtacgggt tgttgaacac cgcgtccacg ttattgatca
     4261 tgttcagcac gttcggcgcg gtcgtccccg cgagcagcag cgcgccgccc gaggtggcgt
     4321 taacgaggcg gtaccacgcg ttcatcggga tcgagaacat cgagcccttc tgccactcga
     4381 acacgtgctt cttcacatcg ccctcgagcc agacctcggt cgtgccgcgc ccttcgacca
     4441 cgaggaagat ctcctcgtac atgtgcttct cggggttgag cgcgcccgcc ggagggacct
     4501 cgacgacgta gcagccccac ttggtctcgg tgccgtgcag ctggatgtaa tggccgcgtc
     4561 cgcccttgcg cttccacggc atgagcggca ggtcctgcac ccggcggatg ccgatgccgc
     4621 ggaacacggg gacgccttcc gactccatga acgcgtcgta aggcgtcggc tgcttcttga
     4681 actggccgaa gccggcgcgc ttgttgccgg tgggctcgtg ccagtgggtc tgatccttgt
     4741 cgtgcggcat ggcggaattt ctcgcggaaa gcatcgaagg ggaagccgcg acatgctatc
     4801 aaagaccgtc gccgcccagc aacagaatgg aatgccgttc gacatggcgg aacgcccgcg
     4861 gtgcgcgcag gacagaccgc gcgcgattgt tctaaactcg ctttccccac cgtcggaccc
     4921 gacatgcgag ccatccttcg tgccgccgtt ctcctgtgct gcctcggccc ggcgctcgcg
     4981 cacgcgcagg ctccctaccc gacccggccg atccgcttca tcgcgccgtt cccgcccggc
     5041 ggctcgagcg acgtgctgtg ccgcctgctc gggcagaagc tcaccgagcg gctcgcgcag
     5101 cccgtgacgg tggaaaaccg cccgggcgcg ggcggcaaca tcggccacga gttcgcggcc
     5161 aagcagccgc ccgacggcta caccatcctg atctcgagct cgagcacgct cgcgaccaac
     5221 ccgcacctgt acaagcacct cggcttcgat ccgatcgccg atttcgcgcc gatctcgatg
     5281 gtcgcgagcg cgggccaggt gctggtggtc aatccctcgg ttcaggcgaa gagcgtcgcg
     5341 gagctggtcg cgctcgccaa ggccaggccc ggtcagctca acttcggctc gggcggcaag
     5401 ggcatccagt cgcacatcag cgcggagatg ttcaaggccg cgaccggcat cgacatcgtg
     5461 cacgtcccgt acaagggcac ggtgcaggcg gtctcggacc tggtcgccgg ccaggtgcag
     5521 atggtgttct ccgacatggt gccggcgatg ccgcacatcc gcgcgggacg cctgcggccg
     5581 ctcgccgtca cgaccgaaaa gcgctcgccg gtcctgcccg aggtgccgac gatgatcgag
     5641 gccgggatcc cgggctacca cgcgggcgtg tggtgggcgg tcgtgtcgcc gcacgggacg
     5701 ccccgcgcga tcgtcgcgcg gctcaacacc gagctcggac gcatcgtgca gctcgccgac
     5761 gtccgcgaga agtacgacgg cctgggcgtc cagaccgagc acagcacgcc ggaggaggtc
     5821 accgcgcgca tcaaggctga cacgctcgag gtcgcgaagg tgctggcggc cgccggcgtg
     5881 gagcccgagt agatgcgtcg cgccatccgc gccgcgggcg cgcttttcgc cgcgacgctg
     5941 ctcgcctcct gcacgggcct catgcggccc gcgggccttg tggctcccga cgccaagtgg
     6001 gaggaggtct cgcacgccgg gcgcgtgtac gccgagggcg tggtcgcggc gaaggacggc
     6061 gccgtgtacg tcagcgatct gacgcgcacc gggctcgtgc gcgagggcaa cccgcgcggg
     6121 acgatctacc gctacgaccc ggcgacgggc acgacgaccc ggtacctcga gccgagcggc
     6181 atggcgaacg gcctgcacgt cgaccgcggc ggcgacctca tcatggcgca ggacgccgac
     6241 ggcggcgggc gcgcggtcgt gcgccgcaac ctcgcgaccg ggcagacgtc gctggtcgcc
     6301 aacgcgtacc gcggcaagcg cctcaattcg ccgaacgacg tcacgagcga cgcgcagggc
     6361 cgcatttact tcaccgacgc gcgctacggc gagcccgagc ccgccgagct cccgaacgcg
     6421 gtataccgct cggatcacga cggtgggcgc ctcgtgcagc tctcgaccga catcctgcgg
     6481 ccgaacggca tcgaggtgtc gcccgacggc aagcgcctct acgtgtcggc gtcgaacacc
     6541 gcgcgcctgc cgcccaaccc gaacggtccg gcgcaggacc gcttcggcat cacgatgggc
     6601 ggcgtcgtcg tctacgatct cgacgccgac ggcaacctca cgaacggccg cgtcttctac
     6661 aagcgcgacg atctcgtcgc cgacgggatg gcgatggaca gcgacggcga cctgtacgtc
     6721 gccttccaca acgccaaccc gcgcgatccc aagggcgcga tcgtggcgct cgatccgggc
     6781 ggtgcgctgc ttgccgagct gcccctgccg cccggcgcgc tgccgaccaa cctcggcttc
     6841 ggtcgcggcg ccgacgcggg aagcttgtac atgacgagcg cggtgccctg gaagctgtac
     6901 cggctcaaga cgctgcggca cggacattat ttcgaacaat gagtttcgag caatgagttt
     6961 cgagtgacgg gaacgacatg agcccgacgc ctttcgagct ggacctgaag ctgcaggtcg
     7021 gcaagtacga catccgcaag atgctcggca agggcgcaac gggcaccgtc tatctcgccg
     7081 tcgacacgtt ctcgggcagg gaggtggcgt tgaagaccat cgagcccgag gtcttccgcg
     7141 atcccgagtt cgggaccgtg caccgcaccc agttcctcgc cgaggcgtcc ctcgccggca
     7201 agctcaagca cccgcacatc gtcggcatcc tggacgcggt ggtgcaggag gactcgggct
     7261 atctcgcgat ggagctcgtc accggcggcg acctgtcgca gtttaccgcg ccggggaagc
     7321 tgcttcccgt cgccgatgtg ctgcagatcg gcttcaagtg ctgtggggcg ctcgactacg
     7381 cgcaccgcga gggcatcgtg caccgcgatc tcaagcccgc gaacatcatg gtggcgaagg
     7441 gcaccgagcc gaagatcgcc gacttcggcg ccgcgctgct gcgcaagacg caggcgatcc
     7501 agacggcgtc gatgggctcg ccgtcgtaca tgtcgcccga gcagctcgag aaccggccgc
     7561 tcaccttcca cagcgacatg tactcgctcg gcgtggtgct gtacgagctg ctcagcgggc
     7621 gcaagccctt cgccgccgac aacatccagt cgctggtgca gaagatcctg cgcgacgatc
     7681 cgccgctgcc gagcgcgctg cgcgccggcg tcccgaaatc ggtcgacgcg atcgtgctgc
     7741 gggcgatggg caagcgcccc gagcaccgct acaagacctg ggccgagttc gcgctcgacc
     7801 tgtcgagcgc ggtgaagctg gtgctgcccg gggaggcggt cgccgacagc gagaagtacg
     7861 ttgcgctgaa gcgcgtcgag atgctgatcg ggctgtccga cgccgagctg tgggagatcg
     7921 ccggggccgc gcgctggatg cgcgtcctcg cgcgcaaggt gatgctgcgc gagaacgagc
     7981 cggggaacag cttcttcttc ctcgcgcgcg ggcaggtcaa ggtcacgcgc cagggccgcc
     8041 tgctgaacat ggtggacgag cgcgagttct tcggcgagat ggcgtacatc tggggcggca
     8101 aggcggcgcg ccacgcgacg gtcgaggcgg ccaccgacct gctggtcgcg gagttcgagc
     8161 ccgaggcggt ggcgaagatg agcgaaggcg cgcagctcca tctcacgcgg gcgctcgcgc
     8221 gcaatctcgc ggaccggctc gagctcgcga acacgcggct cgggcgctga gcgggggctc
     8281 gcgatggacg gtatcgacag ccggcgccgg cgtttccttg ccgccggcgc ggcgctggcc
     8341 gccgccggct gcgcgcagca gcccgcgctg cggccgacgc gctggacggc gcggcagttc
     8401 cacaaccagc ccgcgctcag ccaccagcac cagttcctga ccgacctgtg gctgcaggtc
     8461 tacaaggaga cggacgggcg gctgaacgtc accgtgtacc cgcagaacga ccacatcgag
     8521 ggctccgacc ccgcggcgct cgacatgctg caatcgggcg agctcgagtt cttcaccgtc
     8581 atgggcagca tcctcggtcg caaggtgccc gccgccgaga tccagagcct gccgttcgcg
     8641 ttcaccagcc acgcgcaggt gcaccgcgtg aacgacgggg cgctcggcga gtacctcggg
     8701 cgcgaatgcg ccgccaaggg catctaccgc ttccaggacg gcctgctcga gaacggcttc
     8761 cgccacatca gcatgatcga gcgcccgatc aacgccgcgg aggacctgcg cggcgtgcgc
     8821 atgcgcgtcc cggacggccg gatgttccgc gacctgttcg ccgcgctcga ggccgagccc
     8881 gtcaccgtca acatccgcga cctctacgac gcgctcaaga ctcgccaggt cgacggccag
     8941 gagaacccgc tcgtcgtcac cgaggtgaac aagctctacg aggtgacgcg ctacgtctcc
     9001 atcaccaacc acatgtggtc gggattcaac ctgctcgcca acctcgggtt ctggaacggc
     9061 cttccgggcg atgtgcagga gatcgtccag cgcaacgtgc gcaaggtcgt cgccgcgcag
     9121 cgcgagtaca ccgacggtct caacagggag ctgcagggac gtctcgcctc cgagcgcggc
     9181 atgatcttca acacggccga caccgccagc ttccgggcga agctcggccg cgccttctac
     9241 ggacggtgga gagaccagtt cgggtctacg gcttggagcc ttctcgaaaa cgaagtcggc
     9301 acgctgggct agcgcggccc gcccgccgcc aggcggtccg ctggcgcatc gccctgatcg
     9361 tgctgctcgt gctcggcggc gtggccgcgg tcgcggtcga aagctaccgc gtcgtcgacc
     9421 gcgagctcac cgagtcggcg ctcgagcgcc gcggcgcggt ttcgttcctt gcggccacgg
     9481 tactgtcgga gaagttcgac cggctgctcg acgtcggcgt cgcgctcgcg acgcgcgtgc
     9541 gcttccgcca gctcatcgag gccggccgct gggacgaggc gatccggatc ctgacccggg
     9601 tgccgcgcga tttcccgttc atcgaccgca tcaccatcaa cgacgccagc ggcacgctgc
     9661 gcgcagacat tccgcccgcg cccgagatcc gcggccagaa cttcgcgcac cgcgactggt
     9721 accagtcggt ggagcggacc ggcaggccct acgtctcgcg ggtgtaccgg cgctccgcgc
     9781 cgcccgagcg caacgtcttc gtcgccgcca tcccgatcaa gtcggactcg ggagcgacca
     9841 tcgcggtgct cgtgctgcag gtgcgctcgg atctcttctt cgaatggacg cgcggcgtgg
     9901 aggtgggcga gcgcggcgtc gtctacgtcg tcgaccagcg cggctttctc gcctaccacc
     9961 cgcgccgggc gtcgcagcag gagctggtcg actactcgcg cgtgccggcc gtccagcgcg
    10021 tgctccaggg cgcccgcggc gtcgacgtgg tcagcgatcc cgagacgcgc gagcggcagg
    10081 tggtggccta ccagccgatc ggcgcgcacg gatggggcgt ggtgctcgcc gagcccgccg
    10141 atgccgtgtt cgcgctgcgg aacgaccagc tcaagtggat ggttttcacc tacgtcctgt
    10201 cgatcgtgtc gctcggcgcg ctcgcgtacc tcgcctcgag catcgccttg caccggcggc
    10261 agatcgacgt cgcgaacgag acgcacgccg agctcgagcg gcgcgtgcgc gagcgcacgc
    10321 tcgagctcga gcgcacgaac cgcgagctcg agagcttcag ctactcggtg tcgcacgacc
    10381 tgcgggcgcc ggtgcgcgcg atcgaaggct tcgcgcggat cctggaggag gaggcgggcg
    10441 ggcggctcga cgccgagtcg cgccggtacg tcgcgctggt gcgcgacaac ggccgccgga
    10501 tgggcgtgct gatcgagtcg ctgctcgagt tctcgcggct ggggcggcgc ccgctgaacg
    10561 tcgccgacac cgacatgggc gagctcgcgc gcgacgtgtt cgaggagctg gccggcggcc
    10621 tgccgaaggt tcccgagctc gtcctcggtc ccatgccgcg cgcgcgctgc gaccgcgcgc
    10681 tcgtgcgaca ggtgtggacc aacctgctgt cgaacgccat caagttcagc ggcaagaccg
    10741 acgcgccgcg cgtcgaggcc ggcggcgagg cgaaggacgg cgagaacgtc tatcacgtcc
    10801 gcgacaacgg cgcgggcttc gacatgcgct actacgagaa gatgttcggg gtgttccagc
    10861 gcctgcacgg cgaccaggaa ttccacggca ccggcgtcgg gctcgcgctg gtccagcgcg
    10921 tcgtcgagcg ccacggcggc cgcgtctggg ccgagggcaa ggtgggcgag ggcgcgacct
    10981 tctggttttc gctgcccgcg gccgggaagg cctagccgcg gcttcgcttc gcgaccacga
    11041 atccggcgaa cgccaacccc gcgaacagca tcaaccagac agacgacgac tcggagcccg
    11101 gcgccgagcg ctgctccgcg cgacgctcgt tcgccggcga gcgtgcgacg gggcgcgagg
    11161 gctcggggcg catcacgagg ctcccggggc gagcgtcgga cagggtggcc gcgacccgct
    11221 cgggcgccgt gccggcatcg cccggcgcga gagccggcac cacctcggcc gggatctggg
    11281 cgatctgcac ggcggccgct gcggcgggct cggcgctcgc catgagcgcg ggaaccgtat
    11341 cggggcagcc gtcggaggct tcgcaggcgg cccacgccgg gcaggagagg gcggcgagca
    11401 tggcggcaag gaggtacgac cgaataggca tggtttccac gacagtcaac gcaaccgtcg
    11461 gcgtattctt ggctccgcgc atgacgccaa gccccacccg gggttggggg ccgctggtct
    11521 catcggcatg ggcatgtgcc ggacggcgca tcgcgtcggc cgcgcgatgt atccgcaaat
    11581 acagagacgc gaccgggcgc gcggggcctt tacgcgatct gcgccgcctg gcgcaggctg
    11641 gcgatcatcg acgtctcgag catgtgccgg cgcgcgcgct cgggattcga ggcgatggcg
    11701 acgagctcgg ccaggcgctc gcgctgcacg cgcggatcgt gctcctcgag catgcgcttg
    11761 ttgcggatcg acatcttgtc gacctgctcg atggccaccg tgcggcgctg ccgcgcgtag
    11821 acgtcgagca gcgactcgtc cgcgtcgccg cgccacacct tgcccagctt ctcggcgagg
    11881 ctgacggcgt cgtggatgcc gctgttcagg ccgaaggcgc cgatcgggtt gttgatgtgc
    11941 gccgcgtcgc ccgcgagcag cacgcgtccc gcgcggaacg ctttgcacac gcgctggtgc
    12001 acgcggtagg tgctcttgta gcgcacctcg tagggaacac tgcgcgcgac gaagcgctgc
    12061 agcttgtcct cgacgttctc cggggcgaga accacgtcct cggcctgatc gggcaccggg
    12121 aacaccgcgc gccacaggcc gggcggaccg gagttcggca tcttgaacag cgcgatccac
    12181 tccaccggat ccgcgatgta cgcgttcagc gcgaagccgt agcgcgagaa gtcgtgcgtc
    12241 gtgctcgcga ccaggaactg ctcgggccag gtgaagccct cgaactcgat gcccagcgcc
    12301 ttgcgcaccg agctgcggcc gccgtcggcg ccgatcaccc aggaggcctc gatcgtctcg
    12361 ggcttgcccg cggcttcgac gtgcacgagc acgcggtcgc cgcgcggctc gacgcccgtg
    12421 acggcgtgat cgaagcgcac atccgccccg gcgctgcgga ccaggtcgag ctggatcggc
    12481 gtcagccggt gctgctcgag gtgcagccgg tagggatacg gcgtgtcctc ggcgagcagg
    12541 ccgaggtcga actgcgcgac gaactcgccg cgccggtcgc ggatctgcca gtggcgcacc
    12601 gggatgccga cctcgagcat gcggtcggtg atgccgtagg gcgccatcag ctcaagcgtc
    12661 ggcgggtgga acgtgccggc gcgcaggtcg cgcgcgagag cgcggcccga ctcgcacacg
    12721 acgaccggga tgccctgctg cgcgagcgcg agcgcgcaca tcagcccggt agggccggca
    12781 cctgcgacga cgacgggcag tcgcttgctg tcgatggtcg ttctccggtt gcgctattcg
    12841 gcggtgatgt ggttccggcg gatgatctcg ccccagcgcg cgtaatcccg cttcatgagc
    12901 gcgaacaact cctcgggcgt cgacgaggcc gcgtccaggc cgaccttctg cagcaccgct
    12961 ttcgcctcag gctgcgccag gatggcgcgc agctccctgt tgagcttcgc gatcacgggt
    13021 gccggcacgc cggccggcgc gtagaacgcg taccagatat cggccgcctc ggcgcctttg
    13081 attccgagct cgccgagcgt aggcagatcg ggcgcggaag gatggcgctt cgcgcccgcc
    13141 gcggcgagca gccgcagccg tcccgcgtcc cggtacggca gcacctggtg gatcggcagc
    13201 aacatcacgt tgatctgtcc cgagacgaga tcggtgacgg cgccggcgct gcccttgtac
    13261 ggaacgtgca gcaggtccgc gccggtcagg tccttgaaca gttccatccc catgtgatgc
    13321 ggcgtgccga cgccgggtga gccgtaggtg agcttgcccg gctgcgcttt ggcgcgggcg
    13381 acgagctcac gcacgctccg gatgcccgtg gccgggttga cgacgagcac catggtggtg
    13441 gtcgccgcga gcgtcaccgg cgagaagtcg cgcagcggat cgaacgggac gttcttgtac
    13501 aggttggacg cgatgaccag cgtgttggcg ccgaccatca gggtgtggcc gtcgggagct
    13561 gccttggcga cggcctcggc gccgatgttg ccgctcgcgc ccggccggtt ctcgacgacg
    13621 accggctggc ccagccgccc ggtgagcttg ggtccgacgt gacgggcgat gatgtccatg
    13681 cccgtgcccg gcgtgaaggg cacgatgaac ttgatcggct gcgacggaaa attctgggaa
    13741 gcgggctgac ctgcggcgga tccgcagaca accaggccgg ccaggagcca tgcacgatgc
    13801 atcgggtacc tcgtatcggt cgagtgacaa aaatcacgcg tcggacttcc ggcaacaccg
    13861 ccggaatccc gtcgatttcg gggcctctta cgcccgtctt cggacgtttt tggggatagc
    13921 agcaagccgc aggccagctt tgtcagcttg ccgaaacttt tgcgacgaga gaggggagtc
    13981 agggcgccgc gaggaagccc tgcaccgcgg ccacgaactc ggcgggtttc tcgatctgcg
    14041 ggcagtgccc ggcgcccggg atctcgagga agcgcgcgcc gggaatggct cccgcgagct
    14101 cgcgcgcgag cccgggcggc gtggtctggt cgagcgcccc gaccatgacc agcgtcggat
    14161 tgcgtatcgc gcggaccgcc tcgcgcaggt cgacccgggc gagcgcgagg caggcgcggg
    14221 cgaagcaacc cggatcggcc tcggcgaggg cggccttgcg ctcggccacg acctcggggt
    14281 gctcggcaat gaacgcctcc gggaacatgc gccgcaccgc ggcgtcgagg accgcctcca
    14341 tgccctgctc gctgaccttg gcatggagga ccttgaaagg ctccttcgcc ggcggcggaa
    14401 aggtcgcggc cgagtcggcg atgatcagcc ggtcgaactt cgccccgtgc cgggcggcga
    14461 gcgcgagggc gacgaacccg ccgaaaccgt tgccgagcac cgcggtgcgg gccgagagct
    14521 tcagcgccgg gaacaggccc gcgacgtgat cggcgtactc ctcgatgctc gcgccggccg
    14581 gcgaggaggc gccgtagccc ggcatgttcg cgaggacgac gcggcgctcg cgcgcgagct
    14641 cgccctttac ccggtcgaac gccgagcggt cggcgagcag cgagtgcagg agcaggagct
    14701 cggggccgct gccgatctgc tcgacgtcga tgcgcgcgcc gccgactgcg actgccgtca
    14761 ttccaggttc ttcgcgggcg ggtagtcgcg cgagtaggtc ggctgcgcga ggaactgctt
    14821 cgggtcgtag cggaagaact ggctcacttc gcgcaccgtc tcgatcgtcg tgttgaccag
    14881 ctggccgttc ttgcgctcga ccttgcgcac atagatgttg agcacgggct tgccgtactg
    14941 atccaggcgg atcggaccca tcggtccgtg atcgagcttc acgctgtgca gcgccttgac
    15001 gaacgccgcc ttgtcctcga acttgccctt gatgtccttc agcgcctgct cgagccacag
    15061 gccggccgta tacgtgccgg cggtgtagaa gcccgggatc accttgtact cgcgctggat
    15121 cgactcgacg aagcgcttgt tgtcgggcgt gtcgcggtcg accgtgtacc agctcgccga
    15181 gtacacgccg agcgcctcgt cgctcatgtt cttgaggatg ccttcgtcga ccagcgtcgg
    15241 gttgccgaac accggcttgg tgtgcccgta ctcgcggtag gcgcgcagga agcgcagcgg
    15301 gttggagccc gcgaagccgg cgtacacgcc gtcggcgtcg ggcttgatct gcccgacgta
    15361 ggagccgtag tcgggcacgt tgagcggcgg ccacagcttc tggatgatcc tgccgccgtc
    15421 gtcctcgaac acgcggtgga agcccgcggc gccctcgtgg ccgtaggtga agtcgtccgc
    15481 gaccatcgca accttcttga gcttcagctt tttcgccgcg aagtcgccga gcacgtgcat
    15541 cggctgcgcc gcggtgccgt agacgtgcac cacgtaatcg ctcttgccct gctgcgcgag
    15601 gtccatctgc gcggcggacg tgggcgtgat gagcggagtc ttcgacgaga gcatgtagtc
    15661 gtcgatggcg agggcctcga acgtcgcgag cggtccgatg atgacgtgca cgtggttgcg
    15721 ctcgaccagc tcctgcgtct tgctcttcgc ctgcgccggc tggcccgccg tgtcggcgaa
    15781 atagatctcg accttgcgcc cggcgaaggt gttgttgcgc tccttgagca gcagattgat
    15841 gccttcttcc atctggcggc cgccggccgc gagcgggccg gtgcgcaccg tgaggaagcc
    15901 gatgcgcagc ggctcgccct gcgcgagcgc gctgctcacg ggaagcgcgg tgaccgcgag
    15961 cgcggcgaat gcgaaaagca acgacctgcg tgacgccatg gtgactcctc ctgggggacc
    16021 ggatgccgaa tattcgtgaa cggattgttc tggaccggta tcgtaccgca tcgttagaat
    16081 ggccgtcccg cccacgcgga acgcagccat gcgcccggac ctgctcgccc tcgcctcgag
    16141 cctcgcggcc cgcgaagaag gcttcgcgct ggtgacggtc gtgcggcgcg agccgcccag
    16201 ctccgcgcgc gtcggcgata ccgcgctcgt gaccgcgacc ggcgactatc acggctgggc
    16261 gggcggcggc tgcacgcgct cgaccgtggt gcgcgaggcg ctgcgcgcga tcgccgacgg
    16321 cgagccgcgg ctgctgagcc tcgcgcccga gtcgtccgag cgccaccgcc ccgggctgat
    16381 cgcgctcccc atgacctgcg acagcggagg taccgtggag atctacgtcg agcccgttct
    16441 gccggtgccc cgcctgctgc tgttcggaag cacgccggcc gtgcgcgcgc tggcggccat
    16501 cgcgcgtgcg atgggatatc gcgtcgaggt ggtcgacccg gacgcggcgc ccgcggattt
    16561 tcctggcgcc tcggtgcgca gcgacctctc gcccggcgac atcccgcgcg gcgcgcacgt
    16621 ggtcgtcgcc accatgggcg accgggacct cgagatcctc gacgcggcgc tcgcgagcgg
    16681 gcccgcatac ctcggcgtgg tggcgagccg caagcgcttc gagccgctgc gcgacgcgct
    16741 cgccgcgcag ggcgtgtcgc gcgaggcgct cgagcgcgtc gccgcgcccg ccgggctcga
    16801 cctcggcgcg cgcacgcccg aggagatcgc gctctcgatc atggcgcaga tcgtcgagcg
    16861 ccgccgcgcc ggcgcgcggc cgccgcgcga ggagccgcgg gcgcaacccg cgcgcgaggc
    16921 ggtggacccg atctgcggca tgagcgtcac cgtcgccggc gcacgccaca ccgtggaggc
    16981 gggcggcacg acctggtact tctgctgcgc cggctgccgc gccaagttcc tggccgatcc
    17041 gcagcgccat gcccgggcaa tataagggga cggcgatcca gcggactctc gctgcggcgc
    17101 gtcatgcgcg gaaccgagcg gcaggggttg ctgcgccagt tcgcgcgatg ctatcgtcgt
    17161 ccggcaccct gaaataactc ccgcagtccg tttcgtatcg gcgcaacgcc aaggagaggc
    17221 cgatgattcc cgcttctttc gattaccacc gccccgcgtc cctcgacgaa gcgctcaagc
    17281 tgctgaagaa gcacggcgac gacgccaagg tgctctcggg cgggatgagc ctgctgccga
    17341 tgatgaagct gcggctcgcg tcgttctcgc atctggtcga catcgggcgc atcccgggcc
    17401 tcgacgcgat caaggaggag aagggcgtcg tgcgcatcgg ggcgctggcg cgccaggcgg
    17461 cgctcgagcg ctccgaggtc gtgcgcgcga agctgccgat cctcgccgac gcgctgccgc
    17521 tcatcgccga tccgctggtg cgcaaccgtg ggacgatcgg gggcaacgtc gcgaacggcg
    17581 acccgggcaa cgaccagccc gcgatcatga tcgcgctcgg cgcgaccttc gtcgcgcgcg
    17641 gatccaagga gcgctcgatc gccgcgggcg acttctacaa gggcctttac tcgaccgcgc
    17701 tcgcgccggg cgagatcctc accgaggtcc gcatcccggt gccgcccgcg cggagcggcg
    17761 gcgcctacgt gaagctcaag cgcaagaccg gcgacttcgc ggtggccgcc tccgcggtgc
    17821 agctcacgct cggcaagagc ggcgcgatcg agcgctgcac gatcgcgctc accaacgccg
    17881 gccagacgcc ggtcgaggcg aaggaggccg gccagttcct cgccggcaag acgcccggcg
    17941 acctgacgct gcgcgaggcc gcgcagatcg ccgcgaaggc gtcctcgcct tctgccgacc
    18001 gccgcggctc ggtcgaatac aagaaggaga tggcgcgcgt gctcacggcg cgcgcgctga
    18061 agatcgcgct cgagcgcgcg ggtcgaaact gagaggatct gaccatggcc acccacaaga
    18121 tcagcgtcac cgtcaacggc gcggcgcgcg aggcggaggt ggactcgcgg ctgctgctcg
    18181 tgcacctgat ccgcgagaat ttccgcctga ccgggacgca cgtcggctgc gacacctcgc
    18241 actgcggcgc gtgcaccgtg cagctcgacg ggacgccggt gaagtcctgc acggtgctcg
    18301 cggtgcaggc cgacggctgc aaggtcacga ccgtcgaagg gctggagcag ggcgggaagc
    18361 tccacccggt gcaggaaggc ttcaccgaga agcacggcct gcaatgcggc ttctgcaccc
    18421 ccggcatgct gatgacgacc agcgcgctgc tcgcgcagaa caagaacccg agcgagcagg
    18481 agatccggca ggcgatctcg ggcaaccttt gccgctgcac cggctacgtg aacatcgtga
    18541 aggcggtgca atacgccgcc gagaagatgc gcagcgcctg aaccggagac gaccatgagc
    18601 gactcagtga acgaatcgca gatcaagccg aggaccgacg ccaagatctg cggcatgggc
    18661 cattcgatga agcggaagga ggatccccgc ttcatccgcg gcaagggcaa ctacgtcgac
    18721 gacatccagc tgcccgggat gctgtacatg gacatcgtgc gcagccccca cgcgcacgca
    18781 cgcatcaaga agatcgacac gtcgaaggcg ctgaagatcc cgggcgtgct cgccgtgatc
    18841 gacggcccga cgctcgccaa gtacaacctg cactggatgc cgacgatctc ggccgacacg
    18901 cagatggtgc tgccgatcga cgaggtgatg taccaggcgc aggaagtctg cgcggtgatc
    18961 gccaccgagc gctacatcgc cgccgacggc gtcgacgcgg tcgaggtcga gtacgagccg
    19021 ctgccggtga tggtcgaccc gcacaagtcg ctcgcccccg gcgcgccggt cctgcgcaag
    19081 gacaagaagg acaagaagga caacctcgcg ttccactggg aggcgggcga caaggccgac
    19141 gccgagcgcg cgctcgccgc gtgcgacgtc gtcgtacgcg agaacgtgta cctgccgcgc
    19201 atccatgtcg cgtcgatgga gacctgcggc tgcgtcgccg atttcaactc cgccgagggc
    19261 aagctcacgg tctacatgac gacgcaggcg ccgcacgcgg tgcgcacggt gctcgcgctg
    19321 gtcgcgggtc acgtcggcct gtccgaggag cggatccgag tcgtctcgcc cgacatcggc
    19381 ggcggcttcg gcggcaaggt gccggtgtac ccgggctacg tcgtcgcggt cgcggcgtcg
    19441 gtcgtgatcg ggaagcccgt gaagtggatc gaggaccgca tggagaacct gcaggccgat
    19501 tctttcgcgc gcgactacca catgaaggtc gagctgggcg cgaagaagga cggcaccatg
    19561 accgcgctga agatcagcac ggtcgccgat cacggctacg ccaacgcctc ggcgaacccg
    19621 tcgaagttcc ccgcgggcct gttctcgatc tgcaccggct cgtacgacct caagcacgcg
    19681 ttctgcgagg tcgacgcggc ctacacgcac aagccgccgg gcggcgtcgc ctaccgctgc
    19741 tcgttccgcg tcaccgaagc ggtgcacgcg atcgagcgcg cgaccgacgc gctcgcgcag
    19801 aagctcggca tggacccggc cgacttccgc atgaagaact tcatcaggcc cgagcagttc
    19861 ccgtacaagt cggtgctcgg ctgggagtac gacagcggcg actatccgcg cgcgttgagg
    19921 aaggcgatgg acatcatcgg ctacgacgcg ctgcggcgcg agcaggccga gaagcgcaag
    19981 cgcggcgagc tgatgggcat cggcatctgc agcttcaccg agatcgtcgg cgccgggccg
    20041 tccaagcatt tcgatattct cgggctcaag atgttcgact cggccgagat ccgcatccac
    20101 ccgacgggca aggccatcgc gcgcttcggc accaagagcc agggccaggg ccacgagacc
    20161 acgtacgcgc agatcgtcgc cgaggagctc ggtatcccgg ccgcgcacgt gcaggtcgag
    20221 gagggcgata ccgataccgc gccctacggg ctcgggacct acgccagccg ctcgacgccg
    20281 acctcgggcg ccgcctgcgc gctcgccgcg cgcaagatcc gcgacaaggc gcgcaacatc
    20341 gccgcgcacc tgctggaatg cagcccgcag gacctcgagt gggagccggg caagttctac
    20401 gtcaagggcg cgccgcagat ggcgaagacc atccaggaga tcgccttcgc cgcgtacacg
    20461 aaccacccgc aggggatgga ggcgggcctg gaggcgacgc actactacga tccgcccaac
    20521 ctcacgttcc ccttcggcag ctacatttgc gtggtcgaca tcgaccgcgg caccggcgaa
    20581 gtcaaggtgc gccgcttcgt cgcggtcgac gactgcggca acatcatcaa ccccatgatc
    20641 gtcgacggcc agatccacgg cggcctcacg cagggcatcg ggccggcgct gtacgaggag
    20701 atcagctacg acgaggaggg caacatctcg ggtggcagct tcctcgacta ctacgtgccg
    20761 accgcgctcg agacgccgaa gtgggagacc gacaagacca tcacgccctc gccgcatcat
    20821 cccttcggcg cgaagggcgt cggcgagtcg gcgacggtcg gcgcgccgcc cgcgatacac
    20881 aacgccgtgg tggacgcgct cgcgcatctc ggcgtggcgc acttcgacat cccgatcacg
    20941 cccgaaaaag tgtggcagac gctgcgcgcg aaaggcgtcg ccgagtagcg ctctagtgca
    21001 tgaggagccg ccggtcgagg ccggaccggc gctcgacgcc cgcgtaaggc ggcggcggat
    21061 actgccggcg atcggaacgc gcgccgcgcc ggtcggcgcc gttgtacagc gtctggatca
    21121 gcgccggctc gcgcacgaag cgcgcactcg agcccatgtc gtatcccgtg acctcgccac
    21181 ccggcgcgac gctgaactcg aggcgctcgc cgaagtgcac cgtcgcaaag gcgccgctcg
    21241 agcgcatgac gaagacgtgc gcctccggag tggactcgag gatgacacgc gcggcatggg
    21301 gattgggagc tccgggcggc acgagcgtga gctggccgtc gagcatcgcc acgaacatgg
    21361 tgaggttgcg gttctcggag cggtagaggc cgcagtagcg ggccgcgtcc gcgggcagcg
    21421 cgggaacggt gcgtgcgatc tccttcgcga cgatcggcgc gagcagctgc agcgcgtagt
    21481 cgcagaaggc ggcgggattg ccgtcgtcgg cgttggtgag cacggcgacg cccagcttca
    21541 gcgcggggat gaattccatg agcgccgaga agcccggcgt cgacccgggg tgcccgacgc
    21601 gcacgcgccc gtccgcgcgc cgcacctgca cgccgaggcc ccatgccgtc tgccagtcct
    21661 cgaacagcca ttgcggccgg tgcatctcgc gcagcatccg cgcgccgagg acgccctccg
    21721 cgccgccgag atggaaggcg acgtagcggg ccatgtcctc ggcggtggac accatcccgc
    21781 ccgccgggcg catggcgccg tggtcggcct ggcgcgcggg cgcatggtcg gcgccggggg
    21841 ccggccgcga gtagccggtg gcgatgcgcg ggtcgtcgag ccggggcgtc gtctgcgtgc
    21901 ccgacatccc gagcggggcc aggatgcgcc gctcgatgtg ccgcgcccac ggctcgccgc
    21961 ccaccgcggc gatcagctcc ccgagcagcg cgtagccgac gttggagtac ttccactgcg
    22021 ttcccggggg aaacgtggcg ctgaaatcgc cgggaagcgc ggccgggaag gcgggcgcgc
    22081 tccacatcgt tcccgggagg tcgcgctgca ggcccgaggt gtgggcgagg agctggcgca
    22141 ccgtgacgtc ggcgggaacc ggtgcgtgcg ccgcggccgg gacgtgatcg ccgaagcggt
    22201 cgtcgaggcg cagcttcccg tcctcgacca gctgcagcac cgcgagcgcg gtgaagctct
    22261 tgctcagcga gccgatcttg tacggcgtct gcggcgtagc cggcgtccgc gcctgcaggt
    22321 tcgcgaagcc gtaggcgcgc gtcagcaccg atcggtcgcc ccgcacgacc gcgagcgcga
    22381 cgccgggaat gcgctgctgc gccgccgagt gggcggccca ggtgtcgagc agcgcgagcg
    22441 cctgcgcgag gttgtcggtc atcgcggccg atcatgcagt cgcgcaatgc cgcggtcaag
    22501 cgtaatatcc ccgtcgcccc agtactcgtc gcacattcac gggaggctcc aatgacattc
    22561 ggccgcaacg ctgtgatgct cgccgccctt cccctgttct ggctcgcaat gaacgccatg
    22621 gcccagacac ccgccgcccc gccgcccgtt cccgccgacg tgagcccgct ctacgtgatc
    22681 acctacgtcg agagccgtcc gagcgcgcgc aacgacgcgc aggcgctgct caagtcgtac
    22741 cgcgccgcga gccgcgccgc gccgggcaac ctgcgctcgg tcgtcgtgcg cagcgtcgcg
    22801 cgcccgggac agttcgtcgt cgccagcgcg tggaaggaca aggcggcatg ggatgcgcac
    22861 atggcggcgc ccgcgacgca ggcgtttcgc gagaagctcg ccgcgctgcg caacgcgccc
    22921 gccgacgacc gtttccacaa ttcgctgacg gtcgggccgc tggacaatcc gggcgcggcc
    22981 cggggcgcgg tctacgtggt cacgcacatc gacgtgatcc cgccgcgcaa ggacgacgcg
    23041 gtcgcggcca tcaaagcgtt cggcgagacg gcgcgcgccg cgtcgggcaa cgtccgcttc
    23101 gagatcgtgc agcagaccaa ccgtcccaac cacttcaccg cgttcgaggt gtggcgctcg
    23161 cgccaggcgt tcgacgcgca cggcatgagt gcggcgatgc gcgagttccg cgacaagctc
    23221 tcggggatgc agggcgcgct atacgacgag cgcctctacg agctcgtgga ctgagcgcgc
    23281 gagtccgctg tcgtttccgg gcgacagatc catgcggcta tcgccgtaaa aaattgcgcg
    23341 cacgcgcgcg caacacggac tacgcgcgga attctttcgg cgcgtccctg ctctaagcag
    23401 cgtgctcttt ccggctcacg gaaagatcgt gtcgctccgg agcaacaata tgtggtcctc
    23461 gcgcccgctt tatgacggcc gttacatcgc cgccacgccg cccctggccc gctcgatccg
    23521 ctgggacgac gtcgcgctgc tggtcgcgcg gctgacgctc gccgcgctgt tcctgcccgc
    23581 gggcgtgcag aagcttctgc atatggacca gttcgtcgcg ctcgtcgcgc aatacgattt
    23641 cttcggctac gcgctgccgc gtccgaagtt cttcgcgggc ctcgcggtcg tggtcgagct
    23701 gggcggcggc atcatgctgc tgctcggcct gttcacgcgc tgggcggcgc tcgcgctcat
    23761 cgcgttcctg gtcgtcgcga cggcggcgtc gcaccgctac tgggagtacc gcgcgcccga
    23821 gcgccaggcg cagcagatcg agttccagtc caacatcgcg atcgccgcgg ggctgctcgc
    23881 gctttcggtc gcgggcggcg gcgtgatggg cctcgaccgc cggcgccgca gctactgagc
    23941 ttctagacct cgaacagctc gtcgaccgtg agcggccgcg gcagcatgcc ctggcgcgtc
    24001 gcgtagtcga tcgcgacctc gagcgcgctc cggttcgcct cgacgccgta cggcgccatg
    24061 ttgaactccg agcgcccggg cgcgccttcg gtacgcggag attcggcgcg ccgcgcctcc
    24121 tgcagcacgt cgtagatctc gctcaccacc tcgggcgccg agcgcgcgag ggcggacttc
    24181 acgacgatca tgtgattgat cggcaccgcg cggtgccggc gcgcccagtc cttcgccgcc
    24241 gcctccggat cgcggatcag cggctgcacg cgggcgtcgt cgggcagctc gctgccgatg
    24301 atggcggcgt cgacctcgcc ggcgaggagc atcgcggtca gctccttgcc ctcgggcgcg
    24361 cgctcggcct gcggcggatc gcggtattcg gcgaggtggc cgtcctccat cgtgatccag
    24421 cgcgcgctct cgggcgcgac gccgtgctcc tcggcgagga agccgcgcac ccaggtgacg
    24481 gtcgtctgcg cgtaggcgcg cacgccgatg cggcgtccgg cgaggtcctg cggacgcagc
    24541 gggccgcgct ccgtgttgtg cacgaggcac tggtgctgga agcgcgccgg cccgacgcgc
    24601 gcgggcacga gcacgagcgg ctttccccac gccttcgcct gcaggaaggt catgatcgcg
    24661 agctcggcga cgtcgaacgc gaggtcgcgc accacggcct tgaacgcgcg gttcggcacc
    24721 ttcacgtcgg cgaagtcgag ctcgcactcg gacgggcgga tgcggccctc gcgcagcgcg
    24781 cgcgtgttgg ggtacatgcc gaggagcgtg cgcagcttca tcgcagcgaa gggtagagcg
    24841 tctgcgcggt gcgcgagaag atccactcct ggtattcctt cggcagagag gcgagcgcct
    24901 cgcgcgcgtc ggcgagcagc gccgccagcg tgcccgcgga cgcgggatag ttcgatcccc
    24961 aggcgatgcg cgaggcgccg aactccttca cgacgcgcgc gaagaaggtc tcgggcgtgg
    25021 ccttgccctg gcgcgactcg cgcacgttgt gcgtggtgag cttgaggaaa agattcttgt
    25081 gccgggcgag ctcgagtagc ggcgcggcgt cgagataggg ctcgccgtcg gcgatcgagg
    25141 gacgccccat atgatccagc agcaccttcg cattggggaa gcgcgtgagc acggcgtcga
    25201 gctgcgcata agtgccgcgc atctgtacgc agatcgggag tcccgattcc gaggcgtacc
    25261 gccacgccgg gaaggcgctc ggatcgtcga ggcgcgcgtc cttcgccgcg gcggtgtgcc
    25321 cggcgatgaa cacccgcagg ccccgcagcc tgcggcccgt ccagtgccgg at
//
LOCUS       DATDMW010000345         7158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1096477, whole genome shotgun sequence.
ACCESSION   DATDMW010000345 DATDMW010000000
VERSION     DATDMW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096477"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VI319_14490"
     CDS             complement(<1..470)
                     /locus_tag="VI319_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865104.1"
                     /translation="MERRRALHPERREAAREPRAPEHALRVVRVVHVAHEESGLVVAH
                     EAAQLLGDALQLLHARRDAGLGFVRVLRAREMRGRDEDRLLRGLDAQPRPEQAPLGLE
                     VADFLGAVLGLFQHRAVPVDVALVRPARKRIAHARVVLQHAVEKLLRRALQLLDA"
     gene            complement(639..1142)
                     /locus_tag="VI319_14495"
     CDS             complement(639..1142)
                     /locus_tag="VI319_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY6865105.1"
                     /translation="MRWSHIIVAAAAAATGIGASAVLYSAKPAVPRADFVTLQGEKVS
                     TQSLRGKVVLVNFWSTSCVTCVEEMPKIVDTYRKYRDRGFETVAVAMSYDPPNYVLAY
                     AVSKKLPFKVALDPVGNVARSFGEIEATPTTFVLDKRGRVVARWVGEPDFGKLDALLE
                     TKLKEPA"
     gene            1161..1847
                     /locus_tag="VI319_14500"
     CDS             1161..1847
                     /locus_tag="VI319_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEY6865106.1"
                     /translation="MRRIFAFFGLLVLPAAALALGIEDLSNRDAVAGLKEALTQGAGE
                     AVSRLGVENGFFGDERVKILMPESMKKTESLMRRLGMGKQADELVLRMNRAAEAAVPE
                     ARAVFVDAIKAMSIADARSILAGGDDAATRYFRRTMSDPLAQRFHPIVVRAMDQVRLA
                     ESWDRYASRASRLGLVKKEDANLEDYITRRALDGLFLMIAEEEKRIRANPAQEASSIL
                     RKVFGALIQH"
     gene            complement(1850..2527)
                     /locus_tag="VI319_14505"
     CDS             complement(1850..2527)
                     /locus_tag="VI319_14505"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY6865107.1"
                     /translation="MLALGPLGRRAARAARARKPSMGLVGSGNVGGALGAVWVEARHE
                     VMFSSRDLEHDRALAAKLGALARAGTPREAVAFGDVVMISVPYGALPAVGKELGELLQ
                     GKIVIDTCNPFASRDGEIADWARSKGAGLASAELLPGARLVRAFNAIGADRMGEAHRE
                     PGRVGMPIASDDAAAVAVVMPLIREIGYEPVLIGGLAKGRYLMPGTVLAGEHTAEELR
                     TIAKGLD"
     gene            complement(2571..5309)
                     /gene="polA"
                     /locus_tag="VI319_14510"
     CDS             complement(2571..5309)
                     /gene="polA"
                     /locus_tag="VI319_14510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY6865108.1"
                     /translation="MPDSGSLSEKTLVLVDGSSYLYRAFHALPDLRNASGQPTGAIYG
                     VINMLRKLLADYKADYCAVVFDAKGKTFRDDLYAEYKANRTPMPDDLGTQIEPLCDTI
                     RALGWPMLIIDGVEADDVIGTLARQAEAAGMRCIVSTGDKDLAQLVTDRVTLVNTMSN
                     EKLDRAGVKAKFDVEPGQFLDYQTLIGDSIDNVPGIDKVGPKTAARLIAQYGSLDAIV
                     AHAPEIGGAVGENIRKALDWLPKSKTILTVKCDVPLPFKPSELVLAPRDNAKLAELFA
                     RFGFRSWLREVTAAADAAREIQQQPAPVPVKASTAPRNYELIVTEDALHRWLETMERA
                     ELVAFDTETTSLDPMQALLVGLSFATEAGRAAYVPVEHRYAGAPDQLKIGTVLAILKP
                     WFEDERRRKVAQNAKYDRHVLANHGVEVRGLAHDTLLESYVLESSARHDMDSLAERHL
                     GVKGLSYDQVTGKGAPRIPFDQVELERARDYAAEDADLTLQLHGALHPKIEADEKLAF
                     VYGTIEMPVLSVLFRMERNGVLLDGAQLEAQGRELGGKMMQLEQRAYNEAGQPFNLNS
                     PKQICEILFDKLKIPVIKKTASGAPSTDEEVLEKLALDYPLPKTLLDYRMLSKLKSTY
                     TDKLPRMVNAKTGRVHTNYGQTTAVTGRLASNDPNLQNIPVRTPEGRRIREAFVAPPG
                     HVIVSADYSQIELRIMAHISGDENLLRAFAAGEDIHRATAAEVFGRPLHDVTAEERRY
                     AKVINFGLIYGMSAFGLAQQLGLERATAQAYIASYFTRYPGVAKYMETTREAARERGY
                     VETVFGRRLYLPEIRAGAPARRSAAERAAINAPMQGTAADLIKLSMIAVQDWIERERL
                     GSKLVMQVHDELVLEVPESELVQMKQRLPELMSGVAQLKVPLVVDVGSGPNWEKAH"
     gene            5382..6104
                     /locus_tag="VI319_14515"
     CDS             5382..6104
                     /locus_tag="VI319_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY6865109.1"
                     /translation="MTHKIPKPLTSGGTMQAYFAAEAWRLFGIMSEFVEATERMNEIR
                     PAVSVFGSARVPEGHPYYVLAERTARLLSDAGFAVVSGGGPGIMDASNRGAYAGRSPS
                     VGLNIELPREQFSNAHQDISLAFRHFFARKAMFVKCASAFVFLPGGFGTLDELTEVLV
                     LMQTGKSARVPCILVHAPFWRGLVDWIRGAMVAEKFIGPKDPDLVQLVDEPEAIVETI
                     FRHYEHRSFTPSPSEREVLLNL"
     gene            6114..6446
                     /locus_tag="VI319_14520"
     CDS             6114..6446
                     /locus_tag="VI319_14520"
                     /inference="COORDINATES: protein motif:HMM:NF022639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEY6865110.1"
                     /translation="MLRRISAFAFVLLLALPAAAQQPPKLEPLPEPPPPPPGVDAEQP
                     DDSPVVINGDQEQTEETVVNGQRVVRVTRPGGSVYYLIEDRGDGPGIRNESLDIGLRV
                     PAWVIRQF"
     gene            6459..>7158
                     /locus_tag="VI319_14525"
     CDS             6459..>7158
                     /locus_tag="VI319_14525"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY6865111.1"
                     /translation="MSVFTKVTRAELARWLGDYTIGSPVELQGIAAGVENTNYFVTTT
                     QGRYVLTLFERLSASELPFYLNLMSHLARHGIPCPAPIADRRDQLLGTLKGKPATIVT
                     CLPGTPVLAPQARHCALVGAMLAEMHLAGGGYPGSQENPRGPRWWRETSVSVMPFLDA
                     RRRELLASELAFQQANAHPRLPRGPVHADLFRDNVLFDGERLGGFIDFYFAGVDSFLF
                     DVAVTVNDWCIDSGG"
BASE COUNT         1044 a         2561 c         2395 g         1158 t
ORIGIN      
        1 gcgtccagga gctgcagcgc ccgacgcagg agtttctcga ccgcgtgctg aaggaccacg
       61 cgcgcgtgcg cgatccgttt ccgggctgga cgaacgagag caacatctac tggaacggcg
      121 cgatgctgga agagaccgag cacggcgccg aggaagtcgg ccacctcgag cccgagcggc
      181 gcctgttctg ggcgcggctg cgcgtcaagt cctcgaagca gacgatcttc gtctcgaccg
      241 cgcatctcac gagcccgaag cacgcgaacg aaaccgagac cggcgtctcg ccgcgcgtga
      301 agcagctgaa gcgcatcgcc gaggagctgg gccgcctcgt gcgcgacaac gagcccgctt
      361 tcttcatggg cgacatgaac gacgcgtacc acccgcagcg cgtgctcggg cgcgcggggt
      421 tcacgagctg cttcgcggcg ctcgggatgc agagcccgcc gacgttccat tgctatccga
      481 ccgccgggat cgagccgggc tcaccgacgg tgacggaggc gatcgacctg atcgtcgcga
      541 accggcacgc gcgcgatcgc ggcgagcgtc ccgcagtgct acgccgaagg cctcgcgccc
      601 tcggaccact ggccggtgca ggcgatctac gagctggtct aggccggctc tttgagcttc
      661 gtctcgagca gcgcgtcgag cttgccgaag tcgggttcgc cgacccagcg cgcgacgacc
      721 cggccgcgct tgtcgagcac gaacgtggtc ggcgtcgcct cgatctcgcc gaagctcctc
      781 gcgacgttgc cgacgggatc gagcgcgacc ttgaacggca gcttcttgct caccgcgtag
      841 gcgagcacgt agttcggcgg gtcgtagctc atcgcgaccg ccacggtctc gaagccgcgg
      901 tcgcggtatt tccggtaggt gtcgacgatc ttcggcatct cctcgacgca ggtcacgcac
      961 gaggtcgacc agaaattcac cagcaccacc ttgccgcgca ggctttgcgt cgagaccttc
     1021 tcgccctgga gggtcacgaa atcggcgcgc gggacggccg gcttcgccga atacagcacg
     1081 gccgacgcgc cgatcccggt tgcggcggcc gccgcggcga cgataatgtg gctccagcgc
     1141 agcttcacag ggaacgctcc atgcgccgta ttttcgcctt tttcgggctg cttgtcctcc
     1201 ccgccgccgc gctcgccctc gggatcgagg acctctcgaa ccgcgacgcg gtcgccgggc
     1261 tgaaggaggc gctcacccag ggcgcgggcg aggcggtgag ccggctcggc gtcgagaacg
     1321 gcttcttcgg cgacgagcgg gtcaagatcc tcatgcccga gtcgatgaag aagaccgaga
     1381 gcctgatgcg gcgcctcggc atgggcaagc aggccgacga gctggtcctg cgcatgaacc
     1441 gcgcggccga ggcggcggtg cccgaggcga gagcggtctt cgtcgacgcg atcaaggcga
     1501 tgagcatcgc cgacgcgcgc tcgatcctcg cgggcggcga cgacgccgcg acgcggtact
     1561 tcaggcgcac gatgagcgat cccctcgcgc agcgcttcca cccgatcgtc gtgcgcgcga
     1621 tggaccaggt gcggctcgcc gagagctggg accgctacgc ctcgcgcgcc tcgcgcctcg
     1681 gcctggtgaa gaaagaggac gcgaacctcg aggactacat cacccgccgc gcgctcgacg
     1741 gcctgttcct gatgatcgcc gaggaagaga agcgcatccg cgcgaacccc gcgcaggagg
     1801 cgtcatcgat cctgcggaaa gtcttcgggg cgctgatcca gcactgaggc tagtccagcc
     1861 ccttcgcgat cgtcctcagc tcctccgccg tgtgctcgcc ggcgagcacc gttccgggca
     1921 tcaggtaccg gcccttcgcg agtcccccga tcagcaccgg ctcgtagccg atctcccgga
     1981 tcaagggcat gacgacggcg acggccgcgg cgtcgtcgct cgcgatcggc atgccgacgc
     2041 ggccgggctc gcggtgcgcc tcgcccatcc ggtcggcgcc gatcgcgttg aacgcgcgca
     2101 ccaggcgcgc gccgggcagg agctcggccg aggcgaggcc ggcgcctttg ctgcgcgccc
     2161 agtcggcgat ctcgccgtcg cgcgaggcga acgggttgca ggtgtcgatc acgatcttcc
     2221 cctggagcaa ttcgccgagc tccttgccga ccgcgggcag cgccccgtag ggcaccgaga
     2281 tcatgaccac gtcgccgaac gccaccgcct cgcgcggcgt gcccgcccgc gcgagcgcgc
     2341 cgagcttcgc cgccagcgcg cgatcgtgct cgagatcgcg cgacgagaac atcacctcgt
     2401 gccttgcctc gacccatacc gcgccgagcg ccccgccgac attgcccgac ccgacgaggc
     2461 ccatactcgg tttccttgcc ctcgccgcgc gcgccgcgcg ccgccccagc gggcccagcg
     2521 cgagcatgac caggaaggcg tgaaaaaact tccggcgatc gggcttcacg ctagtgcgcc
     2581 ttctcccagt tcggccccga gccgacgtcg accaccagcg gcaccttgag ctgcgccacg
     2641 ccgctcatca gctcgggcag cctctgcttc atctgcacga gctccgattc tggcacttct
     2701 agcaccagct cgtcatgcac ctgcatcacg agtttcgatc ccaggcgctc gcgctcgatc
     2761 cagtcctgca ccgcgatcat cgagagcttg atcaggtcgg ccgccgtgcc ctgcatgggc
     2821 gcgttgatcg cggcgcgctc ggcggccgag cgccgcgccg gcgccccggc gcggatctcg
     2881 ggcaggtaaa gccgccgccc gaacacggtc tcgacgtagc cgcgctctcg cgccgcctcg
     2941 cgcgtcgtct ccatgtattt cgcgacgccg gggtagcgcg tgaagtagct cgcgatgtag
     3001 gcctgcgcgg tcgcgcgctc caggccgagc tgctgcgcca ggccgaacgc cgacatcccg
     3061 taaatgaggc cgaagttgat caccttggcg tagcggcgct cctcggcggt gacgtcgtgc
     3121 agcggccgcc cgaagacttc cgcggccgtc gcgcggtgga tgtcctcgcc cgccgcgaac
     3181 gcgcgcagca ggttctcgtc gcccgagatg tgcgccatga tccgcagctc gatctgcgag
     3241 tagtcggccg agacgatgac gtgccccggc ggcgcgacga aggcctcgcg gatgcgccgg
     3301 ccctcgggcg tgcgcaccgg gatgttctgc aggttcgggt cgttcgacgc gaggcgcccg
     3361 gtcaccgcgg tggtctgccc gtaattggtg tgcacgcgcc ccgttttcgc gttcaccatg
     3421 cgcggcagct tgtcggtgta cgtcgacttc agcttcgaga gcatccggta atcgagcagc
     3481 gtcttcggca gcggatagtc gagcgcgagc ttctcgagca cctcctcgtc ggtcgagggc
     3541 gcgccgctcg cggtcttctt gatcacgggg atcttcagct tgtcgaacag gatctcgcag
     3601 atctgcttgg gcgagttgag gttgaacggc tgccccgcct cgttgtacgc ccgctgctcg
     3661 agctgcatca tcttgccgcc gagctcacgg ccctgcgcct ccagctgcgc gccgtcgagc
     3721 agcacgccgt tgcgctccat gcggaacagc accgagagca ccggcatctc gatcgtgccg
     3781 tagacgaacg cgagcttctc gtccgcctcg atcttcggat gcagcgcgcc gtgcagctgc
     3841 agcgtgaggt cggcgtcctc ggcggcgtag tcgcgcgcgc gctcgagctc gacctggtcg
     3901 aacgggatgc gcggcgcgcc cttgcccgtg acctggtcgt aggagaggcc cttgacgccg
     3961 agatggcgct cggcgagcga atccatgtcg tgccgggcgc tgctctcgag cacgtaggac
     4021 tcgagcaggg tatcgtgcgc gaggccgcgc acctcgaccc cgtggttcgc gagcacgtgc
     4081 cggtcgtact tcgcgttctg cgcgaccttg cgccggcgct cgtcctcgaa ccagggcttc
     4141 aggatcgcga gcaccgtgcc gatcttcagc tggtccggcg cgcccgcgta gcggtgctcg
     4201 accggcacgt aggccgcgcg gccggcctcg gtcgcgaacg agagccccac gagcagcgcc
     4261 tgcatcgggt cgaggctggt ggtctccgtg tcgaacgcga ccagctcggc gcgctccatc
     4321 gtctccagcc agcgatggag cgcgtcctcg gtgacgatca gctcgtaatt gcgcggggcg
     4381 gtgcttgctt tcaccggcac gggcgccggc tgctgctgaa tttcgcgcgc cgcgtcggcc
     4441 gccgcagtga cctcgcgcag ccagctcctg aagccgaagc gcgcgaacag ctcggcgagc
     4501 ttcgcgttgt cgcgcggcgc gagcacgagc tcgctcggct tgaacggcag cggcacgtcg
     4561 cacttcaccg tgaggatggt cttcgacttc ggcagccagt cgagcgcctt gcggatattc
     4621 tcgccgacgg cgccgccgat ctcgggcgcg tgcgcgacga tcgcgtcgag cgacccgtac
     4681 tgcgcgatca gcctggccgc ggtcttcggg ccgaccttgt cgatgcccgg cacgttatcg
     4741 atcgagtcgc cgatcagcgt ctggtagtcg aggaactgcc cgggctcgac gtcgaacttg
     4801 gccttgacgc ccgcgcggtc gagcttctcg ttgctcatcg tgttgacgag cgtgacgcgg
     4861 tcggtcacga gctgcgcgag gtccttgtcg ccggtcgaca cgatgcagcg catccccgcg
     4921 gcctcggcct ggcgcgcgag cgtgccgatc acgtcgtcgg cctcgacgcc gtcgatgatc
     4981 agcatcggcc agccgagcgc gcggatcgtg tcgcacagcg gctcgatctg cgtgccgagg
     5041 tcgtcgggca tcggcgtgcg gttcgccttg tactcggcgt agaggtcgtc gcggaaggtc
     5101 ttccccttcg cgtcgaacac cacggcgcaa tagtccgcct tgtagtcggc gagcagcttc
     5161 cgtagcatgt tgatcacgcc gtagatcgcc cccgtcggct ggcccgaggc gttccgcagg
     5221 tcgggcagcg cgtggaacgc ccggtacagg tacgacgacc cgtcgaccag gaccagcgtt
     5281 ttttccgaca aggatccgga atccggcatg ttggatagat ggggcgcggc gctcgtctca
     5341 ccgattcgca acttcgtcga gcaacctcga gggaacccgc catgacgcac aagattccga
     5401 agccgctgac ttcaggcggc acgatgcaag cttacttcgc cgccgaggcc tggcgcctgt
     5461 tcggcattat gtcagaattc gtcgaggcga ccgagcgcat gaacgagatc cgcccggccg
     5521 tctcggtctt cggcagcgcg cgcgttcccg agggacatcc gtactatgtt ctcgccgagc
     5581 gcaccgcgcg actgctctcc gacgcgggct tcgccgtggt ctcgggcggc ggcccgggca
     5641 tcatggacgc gtcgaaccgc ggcgcctacg ccgggcgctc gccctcggtg ggcctcaaca
     5701 tcgagctgcc gcgcgagcag ttctcgaacg cgcaccagga catctcgctc gcgttccggc
     5761 acttcttcgc gcgcaaggcg atgttcgtca agtgcgcgag cgcgttcgtg ttcctgcccg
     5821 gcggcttcgg caccctggac gagctgaccg aggtgctcgt gctcatgcag accggcaaga
     5881 gcgcgcgcgt gccgtgcatc ctcgtgcacg cgccgttctg gcgcggcctg gtcgactgga
     5941 tccgcggcgc catggtcgcc gagaagttca tcggcccgaa ggacccggat ctcgtgcagc
     6001 tggtcgacga gcccgaggcg atcgtcgaaa cgatcttccg ccactacgag caccgcagct
     6061 tcacgccctc gccgtccgag cgcgaggtcc tgctcaacct gtaaaatcgg gggatgctca
     6121 gaaggatttc cgcgttcgcg ttcgtcctgc tgctcgcgct gcccgccgcc gcgcagcaac
     6181 cgccgaagct cgagccgctg cccgagccgc ccccgccgcc cccgggcgtg gacgccgagc
     6241 agcccgacga ctcgccggtc gtgatcaacg gcgaccagga gcagaccgag gagacggtcg
     6301 tgaacggaca gcgcgtcgtg cgcgtcaccc ggccgggcgg cagcgtctac tacctcatcg
     6361 aagaccgggg cgacgggccc ggcatccgca acgagagcct cgacatcggc ctgcgcgtgc
     6421 cggcatgggt gatccgtcag ttctgacggg cgcccgccat gtccgtcttc accaaggtca
     6481 cccgcgccga gctcgcgcgc tggctcggcg actacacgat cggatcgccg gtcgagctgc
     6541 agggcatcgc cgccggggtc gagaacacca actacttcgt caccaccacg cagggacgct
     6601 acgtcctcac cctgttcgag cggctttccg cgtccgagct gcccttctac ctcaatctca
     6661 tgtcgcacct cgcgcgacac gggattccct gccccgcgcc catcgccgac cggcgtgacc
     6721 agctcctcgg gacgctgaag ggcaagcccg cgaccatcgt gacctgcctg cccgggacgc
     6781 ccgtgctcgc cccgcaggcg cgccactgcg cgctcgtcgg cgcgatgctc gccgagatgc
     6841 atctcgcggg cggcggctac ccgggctcgc aggagaaccc gcggggcccg cgctggtggc
     6901 gcgagacctc ggtctcggtc atgcccttcc tcgacgcccg gcggcgcgag ctgctcgctt
     6961 ccgagctcgc gttccagcag gcgaacgcgc acccgcgcct gccgcgcggg ccggtacacg
     7021 cggacctgtt ccgcgacaac gtgcttttcg acggcgagcg cctcggcggc ttcatcgact
     7081 tctacttcgc gggtgtcgat tcgttcctgt tcgacgtcgc ggtgacggtc aacgactggt
     7141 gcatcgactc gggcggcg
//
LOCUS       DATDMW010000346         7936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1096957, whole genome shotgun sequence.
ACCESSION   DATDMW010000346 DATDMW010000000
VERSION     DATDMW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096957"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /gene="metG"
                     /locus_tag="VI319_14530"
     CDS             <1..442
                     /gene="metG"
                     /locus_tag="VI319_14530"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEY6865112.1"
                     /translation="RVEEKQLDALFAPEPEKAKMTASTPHPASPGTPTESAGTISIDD
                     FAKVDLRIARIARAEHVEGADKLLKLTLDVGALGTRTVFAGIKSAYDPAKLEGRLTVM
                     VANLAPRKMKFGLSEGMVLAASGEGSGLYLLSPDTGAAPGMKVK"
     gene            572..2599
                     /gene="hyfB"
                     /locus_tag="VI319_14535"
     CDS             572..2599
                     /gene="hyfB"
                     /locus_tag="VI319_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEY6865113.1"
                     /translation="MDLISSPLYGAVAVGVVWLVVAALSLVPAGNAFFARRIAFPLGA
                     LAGLALAALGLEAVGSAPAQMTLPLGLPDLPFHLRVDPLAGFFLLLLGAVSAAISCYA
                     GGYFAGETRGRLALVSAQYHVFLASMAFVVVADDAYLFMVAWETMALSSYFLVTTDHH
                     QPQIRRAGFLYLLIAHLGAIAILLCFGVMQGGQGDYSFDALRAAQLEPRWATVAFVLA
                     FFGFGAKAGMLPLHAWLPEAHPAAPSPVSALMSGVMLKTAIYGMVRVAFDLLGDVRWE
                     WGVLALAVGAGTMLFGILYALMQNDLKRLLAYSSVENIGIVLLGLGLAMVFIGSGHPV
                     AGALGLIAALYHALNHAMFKSLLFLGAGSILHSTGLRNLNHMGGLIRRMPRTAFYALI
                     GSLAISALPPLNGFVSEWLTFQAALQAPLLDDGVLRSLLPLFAAVLALTGALTAMCFV
                     KMYGIAFLGRPREGAGDADAAAHGHADAGPMERFAMAWLAGGCLVLGLFPSTFLLLLN
                     RVVGSLTGHGLSREALESGWLWLVPTASQQASYSPIVFLAVILLVTLLTFFLVRRFYH
                     GRVRFSDPWDCGYPEQTTRMQDSADAFGQPIRHVFGPLYHMERRLPDPDDRPPRFTLR
                     IDDRLWYWLYLPVARLAEVLSGLVGRLQQGRIRTYLLYSFVTLLALLLFVR"
     gene            2605..3555
                     /locus_tag="VI319_14540"
     CDS             2605..3555
                     /locus_tag="VI319_14540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY6865114.1"
                     /translation="MEHLPGVAFQVLQSVLVVLAAPLLMGWVNQCRAWLQNRSAPSLL
                     LPYWTLAKLFHKDAVIARDASAIFRMTPYVIFGCMWLAGGIVPVLATGLPFAPAADII
                     ALVGVFALARVFSALAALDIGTAFGGIGARREMLIGFLAEPAMLMTLFTAAFISGSTQ
                     LTTIVDTLGHRDFAIYPSLAFAAVAFLMVLLAENARIPIDNPTTHLELTMLHEAMILE
                     YSARHLALIEWAMSIKLFAYMTIGIALFLPWGIAPAGDWGALPLAVGALVLKLALAGA
                     GLSVLEAIFAKLRLFLAPEFLSTAFLLAVLGMLMHFLVRG"
     gene            3552..4217
                     /locus_tag="VI319_14545"
     CDS             3552..4217
                     /locus_tag="VI319_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HEY6865115.1"
                     /translation="MSQSMLSQIVNLLAALLLLIAFAMLSQRRILTLIKLLAWQGAAL
                     AASTLIVALETGQTHLYFSAFLTLILKVVMIPWVLHRLIDRLHVRWDIETMLNIPTTM
                     LVGIGLAIFAFALATPISQMASALTRSTLGIALASVLLSFLMMIVRRKAVPQVIGFLS
                     MENGLFLAATSATYGMPLVVELGVALDVLVGTFIFGIFFFHIREQFDSLDIRHMEKLK
                     EED"
     gene            4217..5665
                     /locus_tag="VI319_14550"
     CDS             4217..5665
                     /locus_tag="VI319_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEY6865116.1"
                     /translation="MEVILVLGVPLLGGVLLALTGHKRWAPELNAAMSLATFCASAVL
                     TAKVIADGPMVALEEQFFVDPFNVFLVALTAFVAFTTALFSRPYMRIEQHHGRVNAAR
                     LRLYHSAYQLFTFTMLLCLLSNNVGILWVAMEGATLSTVLLVSLYRTPASLEAAWKYF
                     ILCSVGIAQALFGTILLYFAAEKVLGPGGSSLLWTHLHQVRDQLEPTVLSLAFVFLLV
                     GYGTKAGLVPLHNWLPDAHAEGPTPISAVLSGLLLNVALYAVVRSKVLVDGALGRGFS
                     GGLMMGFGLLSVVVGAFFLSRQRDIKRMFAYSSIEHMGIVTFAFGMGGPVAAFAGLLH
                     MTVHSLTKSAIFFTVGHATQKVGTQNMESIRGLIARSPTIGWGLVLASLAILGVPPFG
                     VFTSEFMMLTTAMHEHSWATPFLLVALGVAFAAIFGKVQPMVFGETTAARLPHPPALL
                     PVFVHLGIVLMLGLYIPPYLVAWYRQAAALIG"
     gene            5662..7212
                     /locus_tag="VI319_14555"
     CDS             5662..7212
                     /locus_tag="VI319_14555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY6865117.1"
                     /translation="MSMRLPGLPVEAQPLPGALAAVHAHVSPGAVRGVCEAVRGERGR
                     LVSLWGSDETTRAGGYALHLALALPSKLLWLSVPLPAARPEFADLSDIFPQADRMQRA
                     ARDLVGIRAVGGDDRKWLRHGAWPEDVHPLRKDFDAAKTFPQEPDRYPFVKVEGEGVH
                     EMPVGPVHAGTIEPGHFRFSIVGEKLLRLEERLGYVHKGIEKRFESMTLEQGAKLAGR
                     VSGDSTVAYAWAYAMAAEGATRTEPPARALALRAVMLELERIANHLGDLGYLGNDVAF
                     SFGFFQFWRLKEELLRRQDQVFGHRYCMDCVVPGGVSRDLPPREAALLAERLDTIESE
                     STTLKSIYEDHAGAQDRFIMTGQVVPDLAERMGLTGLAGRASGQRHDLRVDHPVTPYA
                     QLDVRIAWHGRGDVAARVSVRFDELFESIRLVRKLLDALPQGPVRAPFARAPGERRGI
                     GWVEGWRGDVLVALETDAMGNVHRVHPHDPSWQNWPLVERAVLGNIVPDFPLINKSFN
                     LSYSGQDL"
     gene            7212..7727
                     /locus_tag="VI319_14560"
     CDS             7212..7727
                     /locus_tag="VI319_14560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY6865118.1"
                     /translation="MYYILKQIAKTGIVTEPAPDADAGLRLIEQRLGDAILRRFGRAL
                     AIRHVDAGSCNGCELEIHAASSPHYNLERLGIKFVASPRHADLLLVTGPVSRHMETAL
                     KRTYDATPDPKLVVAIGDCGCDGGIFGESYASCGRISNVIPVDVAVPGCPPPPRAIMQ
                     GILTAVSALRK"
     gene            7727..>7936
                     /locus_tag="VI319_14565"
     CDS             7727..>7936
                     /locus_tag="VI319_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865119.1"
                     /translation="MQLPLGFIGWFFALFSVAALALGVIVIAALARAGAAQRNYLTFS
                     FWNDVLLAGIWVMGLAGGIGVIRLEA"
BASE COUNT         1000 a         2890 c         2657 g         1389 t
ORIGIN      
        1 ccgcgtcgag gagaaacaac tcgacgccct gttcgcgccc gaaccggaga aagcgaagat
       61 gaccgcctcc accccgcacc ccgcctcgcc cggcaccccc acggaaagcg ccggcacgat
      121 ctcgatcgac gacttcgcca aggtggacct gcgcatcgcg cgcatcgcca gggccgagca
      181 cgtcgaaggc gccgacaagc tgttgaagct cacgctcgac gtcggtgcgc tcgggacgcg
      241 cacggtgttc gcaggcatca agtcggcgta cgacccggcg aagctcgagg gtcggctgac
      301 ggtgatggtc gccaacctcg ctccgcgcaa gatgaagttc ggcctgtccg aaggcatggt
      361 gctcgccgcc tcgggcgaag gctccggcct gtatctgctg tcgcccgaca cgggcgccgc
      421 gcccgggatg aaggtgaagt gatctggatc aaattgccgt ccatcggtcc ggggcaccat
      481 cgcctatgat ttcgtccaaa actaatcgtg gcacggcgag gccgggccgc acggcggctt
      541 cccgcgcggg cgagccgccg gtcgggagac gatggaccta atttcttcgc cgctctacgg
      601 cgccgtcgcg gtcggcgtgg tctggctcgt cgtcgcggcg ctctcgctcg ttccggcagg
      661 caacgccttc ttcgcccgcc ggatcgcgtt tccgctcggt gcgctcgccg ggctcgcgct
      721 cgcggcgctc gggctcgagg ccgtcggctc ggcgcccgcg cagatgaccc tgcccctcgg
      781 gctgcccgac ctgccgttcc acctgcgcgt cgatccgctc gccgggttct tcctgctgct
      841 gctcggcgcg gtgtcggccg cgatctcatg ctacgccggc ggctatttcg ccggcgagac
      901 gcgcgggcgg ctcgcgctgg tctcggcgca ataccacgtc ttcctcgcca gcatggcgtt
      961 cgtggtcgtc gcggacgacg cgtacctgtt catggtcgcc tgggagacga tggcgctgtc
     1021 gtcgtacttc ctcgtcacca ccgaccacca ccagccgcag atccgccgcg ccggcttcct
     1081 ctacctgctg atcgcgcacc tgggcgcgat cgcgatcctg ctctgcttcg gcgtcatgca
     1141 gggcggccag ggcgactaca gcttcgacgc gctgcgcgcc gcgcagctcg agccgcgctg
     1201 ggccacggtc gcgttcgtcc tcgccttctt cggcttcggc gccaaggccg ggatgctgcc
     1261 gctgcacgcc tggctgcccg aggcgcaccc cgcggcgcct tcgccggtct cggcgctcat
     1321 gtccggcgtg atgctgaaga ccgcgatcta cgggatggtg cgcgtggctt tcgacctgct
     1381 cggcgacgtg cgctgggaat ggggcgtgct cgcgctcgcc gtcggcgccg gcacgatgct
     1441 cttcggcatc ctctacgccc tgatgcagaa cgacctgaag cggctcctcg cgtactcgtc
     1501 ggtggagaac atcggcatcg tgctgctcgg cctgggcctc gccatggtgt tcatcggctc
     1561 ggggcacccg gtggcgggcg cgctcggcct gatcgcggcc ctgtaccacg cgctgaacca
     1621 cgcgatgttc aagagcctgc tgttcctcgg cgcgggctcg atcctgcatt cgacggggct
     1681 gcgaaatctc aaccacatgg gcgggctgat ccgccgcatg ccgaggaccg ccttctacgc
     1741 gctgatcggg tcgctcgcca tctcggcgct gcccccgctc aacggcttcg tctccgagtg
     1801 gctcacgttc caggcggcgc tgcaggcgcc gctgctcgac gacggcgtcc tgcgcagcct
     1861 gctgccgctg ttcgccgcgg tgctggcgct gaccggcgcg ctcacggcga tgtgcttcgt
     1921 caagatgtac ggcatcgcct tcctgggccg cccgcgcgag ggcgccggcg acgcggacgc
     1981 cgccgctcat ggccacgcgg acgccggacc gatggagcgc ttcgccatgg cctggctcgc
     2041 cggcggctgc ctcgtgctcg ggctgttccc gtcgaccttc ctgcttctcc tgaaccgcgt
     2101 cgtcggctcg ctcacggggc acggcctctc gcgcgaggcg ctggagtcgg gctggctctg
     2161 gctcgttccg accgcatcgc agcaagcgag ctactcgccg atcgtcttcc tcgcggtgat
     2221 cctgctcgtc acgctgctca cgttcttcct cgtgcgccgc ttctatcacg gccgcgtccg
     2281 cttctcggat ccgtgggatt gcggctaccc cgagcagacg acgcgcatgc aggactcggc
     2341 cgacgcgttc ggacagccga tccgccacgt gttcgggccc ctctaccaca tggagcggcg
     2401 gctccccgat cccgacgacc ggccgccgcg gttcaccctc cggatcgacg accgcctgtg
     2461 gtactggctg tacctgccgg tggcgcggct cgccgaagtg ctctcggggc tcgtcggccg
     2521 gctgcagcag gggcgcatcc gcacctacct gctgtacagc ttcgtcacgc tgctcgcgct
     2581 gctcttgttc gtccggtgag cgccatggag cacctgccgg gcgtcgcgtt ccaggtcctg
     2641 cagtcggtgc tggtcgtgct cgcggcgccg ctgctcatgg gctgggtcaa ccagtgccgc
     2701 gcgtggctgc agaaccgctc ggcgccgagc ctgctgctgc cgtactggac cctcgcgaag
     2761 ctcttccaca aggacgcggt gatcgcgcgc gacgcctctg ccatcttccg catgacgccc
     2821 tacgtgatct tcggctgcat gtggctcgcg ggcggcatcg tccccgtgct cgcgaccggg
     2881 ctgccgttcg cgccggcggc cgacatcatc gcgctggtcg gggtgttcgc gctcgcgcgc
     2941 gtgttctcgg cgctcgccgc gctcgacatc ggcaccgcct tcggcggcat cggcgcccgg
     3001 cgcgagatgc tgatcggctt cctcgccgag ccggcgatgc tcatgacgct cttcaccgcc
     3061 gcgttcatct ccggctcgac gcagctcacc accatcgtcg acacgctcgg ccaccgcgac
     3121 ttcgcgatct accccagcct cgcgttcgcg gcggtcgcct tcctcatggt gctgctcgcg
     3181 gagaacgcgc gcatccccat cgacaacccg accacgcacc tcgagctcac gatgctgcac
     3241 gaggcgatga tcctcgagta ctcggcgcgc cacctcgcgc tgatcgagtg ggcgatgtcg
     3301 atcaagctgt tcgcctacat gacgatcggc atcgcgctgt tcctgccctg ggggatcgcg
     3361 cccgcgggcg actggggcgc gctgccgctc gcggtcggcg cgctcgtgct caagctcgcg
     3421 ctcgcgggcg cggggctgtc ggtgctcgag gcgatcttcg cgaagctgcg cctgttcctc
     3481 gcgcccgagt tcctgtcgac cgcgttcctg ctcgcggtgc tgggcatgct catgcacttc
     3541 ctggtgcgcg gatgagccag tcgatgctct cgcagatcgt caacctgctc gcggcgctgc
     3601 tcctgctcat cgccttcgcg atgctctcgc agcgcaggat cctgacgctc atcaagctgc
     3661 tcgcgtggca aggcgcggcg ctcgcagcct ccacgctgat cgtcgcgctc gagaccggac
     3721 agacgcacct gtacttctcg gcgttcctga cgctgatcct caaggtcgtg atgatcccct
     3781 gggtgctgca ccgcctgatc gaccggctgc acgtgcgctg ggacatcgag accatgctca
     3841 acattccgac gacgatgctg gtcggcatcg gcctggcgat cttcgcattc gcgctcgcca
     3901 cgccgatctc gcagatggcg agcgcgctga cgcgctcgac gctcggcatc gcgctcgcga
     3961 gcgtgctgct ctccttcctc atgatgatcg tgcggcgcaa ggccgtgccg caggtgatcg
     4021 gcttcctgtc gatggagaac ggcctgttcc tcgccgcgac cagcgccacc tacggcatgc
     4081 cgctcgtggt cgagctcggg gtcgcgctcg acgtgctggt cgggacgttc atcttcggca
     4141 tcttcttctt ccatatccgc gagcagttcg acagcctcga catccggcat atggaaaagc
     4201 tgaaggaaga ggactgatgg aagtcatcct cgtcctcggc gtccccctcc tcggcggcgt
     4261 gctgctcgcc ctcaccgggc acaagcgctg ggcgcccgag ctgaacgcgg ccatgagcct
     4321 cgcgaccttc tgcgcctccg cggtcctcac cgcgaaggtg atcgccgacg gcccgatggt
     4381 ggcgctcgag gagcagttct tcgtcgaccc gttcaacgtg ttcctggtcg cgctcaccgc
     4441 cttcgtcgcg ttcacgacgg cgctgttctc gcgcccgtac atgcgcatcg agcagcacca
     4501 cgggcgagtc aacgccgcgc gcctgcggct ctaccacagc gcgtaccagc tgttcacgtt
     4561 cacgatgctg ctgtgcctgc tctcgaacaa cgtcggcatt ctctgggtgg cgatggaggg
     4621 cgcgacgctc tcgaccgtgc tgctcgtcag cctgtaccgc acgccggcga gcctcgaggc
     4681 cgcgtggaag tacttcatcc tgtgctcggt cgggatcgcg caggcgctgt tcggcacgat
     4741 cctgctctac ttcgccgccg agaaggtgct ggggccgggc ggctcgtcgc tgctgtggac
     4801 gcacctgcac caggtgcgcg accagctcga gccgacggtg ctctcgctcg ccttcgtctt
     4861 tctcctggtc gggtacggga ccaaggcggg cctcgtgccg ctgcacaact ggctgcccga
     4921 cgcgcacgcg gaaggcccga cgccgatctc ggcggtgctc tcggggctgc tcctcaacgt
     4981 cgcgctctac gccgtcgtgc gcagcaaggt gctggtcgac ggcgcgctcg ggcgcggctt
     5041 ctcgggcggg ctcatgatgg gcttcggcct gctctcggtg gtggtcgggg cgttcttcct
     5101 ctcgcgccag cgcgacatca agcgcatgtt cgcgtactcg tcgatcgagc acatgggcat
     5161 cgtcaccttc gccttcggca tgggcggccc ggtcgccgcg ttcgcggggc tgctgcacat
     5221 gacggtgcac tcgctcacca agagcgcgat cttcttcacg gtcggccacg cgacccagaa
     5281 ggtcggaacg cagaacatgg agagcatccg cggcctgatc gcgcgcagcc cgacgatcgg
     5341 ctggggcctc gtcctcgcga gcctcgccat cctgggcgtg ccgccgttcg gcgtgttcac
     5401 gagcgagttc atgatgctca cgacggcgat gcacgagcac tcgtgggcga cgccgttcct
     5461 gctggtcgcg ctcggcgtcg ccttcgccgc gatcttcggc aaggtccagc cgatggtgtt
     5521 cggcgagacc acggcggcgc ggctgccgca cccgccggcg ctgctgccgg tgttcgtgca
     5581 cctggggatc gtgctgatgc tcgggctcta cattccgccc tacctcgtcg cctggtaccg
     5641 ccaggcggcg gcgctgatcg gatgagcatg cgcctgcccg ggctccccgt cgaagcgcag
     5701 cctctgcccg gcgcgctcgc agcggtccat gcccatgttt ctcccggggc cgtgcgcggc
     5761 gtgtgcgagg cggtgcgcgg agagcggggc cgcctggtct cgctctgggg cagcgacgag
     5821 accacgcgcg cgggcggcta cgccctgcac ctcgcgctcg cgctgccctc gaagctgctc
     5881 tggctgtccg tgccgcttcc ggctgcgcgt ccggagttcg ccgatctctc ggacatcttt
     5941 ccgcaggccg accggatgca gcgcgccgcg cgcgacctcg tcggcatccg cgccgtcggc
     6001 ggcgacgacc gcaagtggct gcgccacggc gcctggcccg aggacgtcca tcccctgcgc
     6061 aaggacttcg acgcggcgaa gacgtttccg caggagcccg accgctaccc gttcgtgaag
     6121 gtcgagggcg aaggcgtgca cgagatgccg gtggggccgg tgcacgcggg cacgatcgag
     6181 cccgggcact tccgcttctc gatcgtcggc gagaagctgc tgcgcctcga ggagcgtctc
     6241 ggctacgtgc acaagggcat cgagaagcgc ttcgagtcga tgacgctcga gcagggcgcg
     6301 aagctcgccg ggcgcgtgag cggcgactcg accgtggcgt acgcctgggc ctacgcgatg
     6361 gccgcggagg gcgcgacgcg caccgagccg ccggcgcgcg cgctcgcgct gcgcgcggtg
     6421 atgctcgagc tcgagcgcat cgccaaccat ctcggcgacc tcggctacct cggcaacgac
     6481 gtcgcgttct cgttcggctt cttccagttc tggcgcctga aggaggagct gctgcgccgg
     6541 caggaccagg tcttcgggca ccgctactgc atggactgcg tcgtcccggg cggggtgtcg
     6601 cgcgacctgc cgccccgcga ggcggcgctg ctcgcggagc ggctcgacac gatcgagagc
     6661 gagtccacca cgctgaagtc gatctacgag gatcacgcgg gcgcgcagga ccgcttcatc
     6721 atgaccggcc aggtcgtgcc cgatctcgcc gagcgcatgg ggctgaccgg gctcgccgga
     6781 cgcgcgagcg gacagcgcca cgacctgcgc gtcgaccacc cggtgacgcc gtacgcgcag
     6841 ctcgacgtgc gcatcgcgtg gcacggccgc ggcgacgtcg ccgcgcgtgt ctcggtgcgc
     6901 ttcgacgagc tgttcgagtc gatccggctg gtgcgcaagc tgctcgacgc cctgccgcag
     6961 gggcccgtgc gcgcgccctt cgcgcgcgcg ccgggcgagc ggcgcggcat cggctgggtc
     7021 gagggctggc gcggcgacgt gctggtcgcg ctcgagaccg acgcgatggg caacgtccac
     7081 cgcgtccatc cgcacgatcc gtcgtggcag aactggccgc tggtcgagcg cgcggtgctc
     7141 ggcaacatcg tcccggactt cccgctgatc aacaagtcgt tcaacctgtc gtacagcggg
     7201 caggacctct gatgtactac atcctcaagc agatcgcgaa gaccggcatc gtcaccgagc
     7261 ccgcgcccga tgcggacgcc gggctgcgcc tgatcgagca gcgcctcggc gacgcgatcc
     7321 tgcgccgctt cgggcgcgcg ctcgcgatcc gccacgtcga cgccggatcg tgcaacggct
     7381 gcgagctgga gatccacgcg gccagcagcc cgcactacaa cctcgagcgc ctcggcatca
     7441 agttcgtcgc cagcccgcgc cacgccgacc tgctgctcgt caccgggccc gtgtcgcgcc
     7501 acatggagac cgcgctgaag cggacctacg acgcgacgcc cgacccgaag ctcgtcgtgg
     7561 ccatcgggga ctgcggctgc gacggcggca tcttcggcga gagctacgcg agctgcgggc
     7621 gaatctcgaa cgtcatcccg gtcgacgtcg cagtgcccgg gtgcccgccg ccgccccgcg
     7681 cgatcatgca gggcatcctc accgcggttt ccgcgttacg caagtgatgc agctcccgct
     7741 cggattcatc ggctggttct tcgcgctgtt ctcggtcgcg gcgctcgcgc tcggcgtgat
     7801 cgtaatcgcc gccctcgcgc gcgccggcgc ggcgcagcgc aactacctga cgttctcgtt
     7861 ctggaacgac gtcctgctcg cgggcatctg ggtgatgggc ctcgccggcg gcatcggcgt
     7921 gatccggctc gaggcg
//
LOCUS       DATDMW010000347         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1595242, whole genome shotgun sequence.
ACCESSION   DATDMW010000347 DATDMW010000000
VERSION     DATDMW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595242"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VI319_14570"
     CDS             complement(<1..252)
                     /locus_tag="VI319_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY6865120.1"
                     /translation="MNVVILAAGHGKRMHSDTPKVLHALAGRPLLAHVIGTAKSLSPS
                     AVVVVYGHGGESVRDAIGAAGLDWVKQEPQLGTGHAVQQA"
     gene            complement(266..1717)
                     /locus_tag="VI319_14575"
     CDS             complement(266..1717)
                     /locus_tag="VI319_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY6865121.1"
                     /translation="MGKITGFLEYQRLQEATENKDARKKHYHEFVLHLADNEASVQGA
                     RCMDCGIPFCMTGCPVNNIIPDWNDLVYRQDVRAALDVLHQTNNFPEFTGRVCPAPCE
                     AACTLNINNDPVGIKSIEHYIADKGWEGGWVVPLPPRRRTGKRVAVVGSGPAGLACAQ
                     QLARAGHEVVVLEKADRIGGLLRYGIPDFKMEKGHIDRRMEQMRAEGVEFRTNQDVGG
                     QGPNAVAAKDVLAEYDAVVLAGGAEQPRDLPVPGRELSGVHFAMDYLPQQNKVVAGDR
                     VEGQIMATGKHVVVIGGGDTGSDCVGTSNRQGAASVTQFELLPQPPEQENKPLTWPYW
                     PYKLRTSSSHEEGAARDWSVATKRFEGANGKVAKLIAARVEMKDGKLVEVPGSEFEIK
                     ADLVLLAMGFLGPVGSVLDAFGVDKDARSNARARTEDGAEGERSYATSVPKVFAAGDM
                     RRGQSLVVWAIREGRQCARSVDEFLMGSSELPR"
     gene            complement(1750..>2213)
                     /locus_tag="VI319_14580"
     CDS             complement(1750..>2213)
                     /locus_tag="VI319_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865122.1"
                     /translation="AGERFCVRNRGVHAVVEGVGDHGCEYMTGGTVVVLGGTGRNFAA
                     GMSGGIAYVFDEDGEFVKRCNTAMVDLEPVLSESEQQAKIARELWHLGEADELVLRRL
                     IESHARSTGSRRAHELLESWGMMRLRFVKVFPKEYRRALTELAASGKKAAA"
BASE COUNT          304 a          814 c          718 g          377 t
ORIGIN      
        1 cgcctgctgt accgcgtggc cggtcccgag ctggggctcc tgcttgaccc aatcgaggcc
       61 ggcggcgccg atcgcgtcgc gcacggactc cccgccatgg ccgtacacga ccacgaccgc
      121 ggacggcgac aggctcttcg ccgtcccgat cacgtgcgcg agcagcggcc gcccggcgag
      181 cgcgtgcagc accttcggcg tgtccgagtg catgcgcttg ccgtgcccgg cggcaaggat
      241 cacgacgttc atcggaggaa gtgtatcagc ggggaagttc cgacgagccc atgaggaact
      301 cgtcgaccga gcgggcgcac tgacgcccct cccggatcgc ccagaccacg agcgactgcc
      361 cgcggcgcat gtcgccggcg gcgaacacct tcggcacgga cgtggcgtag ctgcgctcgc
      421 cctcggcgcc gtcttcggta cgcgccctgg cgttgctgcg cgcgtccttg tcgacgccga
      481 aggcgtcgag caccgagccg accggcccga ggaagcccat cgcgagcagg accaggtcgg
      541 ccttgatctc gaactccgac ccgggcacct cgacgagctt cccgtccttc atctccacgc
      601 gtgccgcgat cagcttcgcg acctttccgt tcgcgccttc gaagcgcttc gtcgccacgg
      661 accagtcgcg ggcggcgcct tcctcgtgcg acgacgacgt ccgcagcttg tacggccagt
      721 acggccaggt gagcggcttg ttctcctgct cgggaggctg cggcagcagc tcgaactgcg
      781 tgaccgaggc ggcgccctgg cggttcgaag tgccgacgca gtccgatccc gtgtcgccgc
      841 cgccgatcac gacgacgtgc ttgccggtcg ccatgatctg tccctcgacg cggtcgcccg
      901 cgacgacctt gttctgctgc ggcaggtagt ccatcgcgaa gtgcaccccc gacagctcgc
      961 gtcccgggac gggcaggtcg cgcggctgct cggcgccgcc cgcgagcacg accgcgtcgt
     1021 actcggcgag cacgtccttc gcggcgaccg cgttcgggcc ctggccgccg acgtcctggt
     1081 tcgtcctgaa ctccaccccc tcggcgcgca tctgctccat gcggcggtcg atgtggccct
     1141 tctccatctt gaaatcgggg atgccgtagc gcagcagccc gccgatgcga tcggccttct
     1201 ccagcaccac gacctcgtgc ccggcgcgcg cgagctgctg cgcgcacgcg agccccgcgg
     1261 gacccgagcc gacgaccgcg acgcgcttgc cggtccttcg cctgggcggc agcggcacga
     1321 cccagccgcc ttcccagccc ttgtcggcga tgtagtgctc gatcgacttg atgccgaccg
     1381 ggtcattgtt gatgttgagt gtgcaggcgg cctcgcacgg cgcggggcac acgcgtccgg
     1441 tgaactcggg aaaattgttc gtctggtgca agacgtcgag cgccgcgcgc acgtcctggc
     1501 ggtacaccag gtcgttccag tcgggaatga tgttattgac cgggcagccg gtcatgcaga
     1561 acgggatgcc gcagtccatg cagcgcgcgc cctgcaccga cgcctcgttg tcggccaggt
     1621 gcagcacgaa ctcgtggtaa tgcttcttgc gcgcgtcctt gttctcggtc gcttcctgaa
     1681 gccgctggta ttccagaaac ccggtgatct ttcccattct cggttcttgc ccgtccccgt
     1741 ttgttcgtat tacgcggcgg ccttcttgcc cgatgccgcc agctcggtga gcgcgcgacg
     1801 gtattccttc gggaagacct tcacgaagcg cagccgcatc atcccccacg attccaggag
     1861 ctcgtgcgcg cggcggctgc cggtgctgcg cgcgtggctc tcgatcaggc gccgcagaac
     1921 gagctcgtcg gcctcgccca ggtgccagag ctcgcgcgcg atcttggcct gctgctcgga
     1981 ttccgagagc acgggctcga ggtcgaccat cgcggtgttg cagcgcttga cgaactcgcc
     2041 gtcctcgtcg aagacgtagg cgatgccgcc cgacatgccg gcagcgaagt tgcggcccgt
     2101 gccgccgagc acgaccaccg tgccgcccgt catgtactcg cagccgtggt cgcccacccc
     2161 ttccaccacc gcgtgcacgc cgcggttgcg gacgcagaac ctctcgcccg cca
//
LOCUS       DATDMW010000348        16072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2344069, whole genome shotgun sequence.
ACCESSION   DATDMW010000348 DATDMW010000000
VERSION     DATDMW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16072
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2344069"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /locus_tag="VI319_14585"
     CDS             <1..1663
                     /locus_tag="VI319_14585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6865123.1"
                     /translation="WAGYEYVREMERFLLDGQEQALLATARAIATALHDRPGLMAPRV
                     EREADPRVRAEQELARLAAQRGEAGGAGDPPKPDAAAPDGSAPPAPTAPRPPSIIARE
                     SSPRDEPREIADILRGVQHTTSRVWVVSRDLRVLALAGTLRRPEGDENEGWAHRVLGE
                     LFPRPSENFDDTIGYDALANGREIAAALRGVPDVGLRGTPDGQAVVMTAAHPVWSGDD
                     VAGAVVAEETTNSILTARSRALERLLVVTLAVFAVAAGVLIWLATGISSRIRRLRDEA
                     ETAIDARGRLAHVVTAQRSGDEIGDLSRSFSALLGKLGQYNAYLEGLASRMSHELRTP
                     IAVVRSSLENLKLSPTAEEARVYIGRAEDGLRRLGTILTRMSEATRLEQGLSSLERER
                     FDLAAVVRGSVDGYRLAYPDRRFELHLPGVPVPVSGSADLAAQLLDKLVANAADFSTS
                     GDPILVRLDAAGNAARLRVENRGPLLPETMRAKLFEAMVSVRGDRADKPGAEPHLGLG
                     LYIARVIAEFHGGAMAAENLASGDGVAVTASFRVAPEPKALAPTR"
     gene            complement(1653..3062)
                     /locus_tag="VI319_14590"
     CDS             complement(1653..3062)
                     /locus_tag="VI319_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6865124.1"
                     /translation="MPSLLLIEDEDTLAKNVQRYLARQGWDVELAGTAESGLAQLAKL
                     QPDVVVLDFQLPGMDGRAALATIRERDPDARVVMVTGQSSVQLAVDAMKAGAADFLPK
                     PLVLADLKRVLDKLAGEGRMRKALAYYHARDQGCMSQILGESPAMIELKERIRLVLRA
                     EAGDTGGPAPAILITGETGSGKEMIARACHSESRRKDGPFIEINCAAIPANLLEAELF
                     GYEKGAFTGANERKIGLIEAADGGTLFLDEIGEADASTQAKLLKVIEEQRIRRVGSVQ
                     ERRVDVRMIAATNQLLEERAREGKFRPDLLYRLRVIELKVPPLRARGEDILLLANRFL
                     GEFAKRYGKPGLAFTPAALDRLTRHDWPGNVRELRNLIEQAVLLARGATIDYSDLGLP
                     AVETCSAAAAPEPEAPQVTLELAQVERDLLRQALERTAWNVTQAARILGITRDTLRSR
                     IEKHGLARPAEPSAPVVNA"
     gene            complement(3064..4422)
                     /locus_tag="VI319_14595"
     CDS             complement(3064..4422)
                     /locus_tag="VI319_14595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6865125.1"
                     /translation="MPLDPLKRPFNLLRWFSLASLVVIAGIAVLNAIVLSDFLTKRLL
                     EREGHVTRDIVQNLLSADGSYAYLDHPDDPALAKRFRGTIDHFNAMRDVIRNNVYSRS
                     KVMLWSSDPELIGKELGENDELDDALKGELVINSGRITDELRKKPEHVGLHPESDFFV
                     ETYIPVRDPEGTRVLGVVEIYKAPVELTAAIIEGRKQVWAAAMLGALVLYVTLYWVVR
                     RGNETIRRQQERLHEAETLSTVGELASSVAHNIRNPLASIRTSAELALEMKGEDCTEQ
                     ARDIMTQVDRVEAWMRELLRFSRQEPGQRAPVDVAALVRKCFNDMARDFERRNLVATV
                     ADEGQGARIDADSTLLEHALHSVVANAVDATPDGGRIEGKVARNGAGRVQIRIRDTGK
                     GIAPADLPHVFRPFYTTKPHGFGLGLAQVRRAVERFGGQVKIDSAPGAGTTVTLELPR
                     AV"
     gene            4638..4805
                     /locus_tag="VI319_14600"
     CDS             4638..4805
                     /locus_tag="VI319_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865126.1"
                     /translation="MLKSNKVRVLAIAVALMGAMGAVAIPAQARSAKCFATPVPGQPG
                     VEIVTCTTTRP"
     gene            4945..5895
                     /locus_tag="VI319_14605"
     CDS             4945..5895
                     /locus_tag="VI319_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865127.1"
                     /translation="MRLLVLLAALAAPLAHAVTPGAARSQSQDVFGGKLETGLTYEVT
                     RANEIKLGDGPVLLLPGDPVRKSDTYRHFAGGANLNAPCGAQLKCVANASVDSRTGVR
                     SSEFDTAIAEGDAGVQYTAGSVVYGLRGGRQQWDVGGQSFRRVNTLVAEAVSAFSRQL
                     SAYGALNVSRYEHPGENAFLDADYRAVTGNLRWGAQDRWQSAYTAQLTLSREDNRQQD
                     PTLDVRGTLIRLAWDAKPAARWAVHPALMFQRLRFGDVDPLFGVRRDDRFTQAELVLN
                     YRIGESLQMRFELMRSRYRSSAAVIDNDWSSVGAGVQWEF"
     gene            5921..7426
                     /gene="glpD"
                     /locus_tag="VI319_14610"
     CDS             5921..7426
                     /gene="glpD"
                     /locus_tag="VI319_14610"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY6865128.1"
                     /translation="MANYDLLILGGGINGAGIARDAAGRGLKVLLVERGDLAHATSSA
                     STKLIHGGLRYLEYFEFRLVAEALAEREALLKIAPHIVWPLEFVLPHEKHLRPAWMIR
                     AGLFLYDHLGHRYFGGRSSLPHSRAVPLDAEGFGAGLKREFARGFAYHDCWVDDARLV
                     VLNARSAAAHGAEIATRTRLAHARREGREWVARLESRSGGREVRAAALVNAAGPWVKQ
                     VLAGVLGVDIPAKVRLVKGSHIVVPRIHAKRHAFILQNPDRRVIFLIPYEREFTLVGT
                     TDIAVGDHDFEAKISPEEIEYLCAAASRYTERPIAPAQVVWSYSGVRPLYDDGSRDPS
                     AVTRDYHLLVDEGSEGREAPLVSVFGGKITTYRRLAEHAMEKLGRRFPGTRAWTRTEP
                     LPGGDFRDRSFTEVFDDCVRRHPGLPAPWLARLLRRHGTLADAVIGDASSRPGLGEDF
                     GGGLVQREVDYLVAHEWAREDDDVLWRRTKCGLHMSAAQRSRVRDYLSSIG"
     gene            7434..8228
                     /locus_tag="VI319_14615"
     CDS             7434..8228
                     /locus_tag="VI319_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY6865129.1"
                     /translation="MGWWLGYLVTGAVAGFFTGMLGIGGGSIIVPMLVALFGAQDLPR
                     AHLMHLAVGTALATFLFTSVASVRAHAQRGAIRWDIARRMTPGILAGGVIGSFLSRYV
                     STAGFAAIFTLVIYAAATSMLIDIRPRASRDLPGTAGMFAAGFLFSGIAAFAAVGGAF
                     MTIPFMMWCGVTLIEAIGTASLIGLPIALSGSVGFMIAGFGEQALPAWSVGYVYLPAL
                     AGVVVAGVLAAPLGAAVAHRTPTRWLRRVFAVLFFVVATRMLIALW"
     gene            complement(8236..8943)
                     /locus_tag="VI319_14620"
     CDS             complement(8236..8943)
                     /locus_tag="VI319_14620"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6865130.1"
                     /translation="MKRVHGPPHLRVGPVVLAVAVCAAAVAISGRTSSWSPAALVPGF
                     VLSEWKSGLEHDGREVYDQRMRIVDAVGLRPGMTVADIGAGTGLFARLFARRVGPTGR
                     VYATDVSRWAVLKTTVLARAEHLGNLTAVVNGEADTGLPPASLDVAFLCDVYHHFGKP
                     WTMLHSIYRSLKPGGRLVLVDYDRVPGRSPAWILEHVPARFDKASVLRDFEEAGFRLR
                     DEETFLSENYLVVFQRP"
     gene            complement(9041..10261)
                     /locus_tag="VI319_14625"
     CDS             complement(9041..10261)
                     /locus_tag="VI319_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY6865131.1"
                     /translation="MGPLSHIRVLDLSRVLAGPWAGQNLADLGAEVIKVERPGTGDDS
                     RAFGPPWVKDAAGRDTKDSAYYTSANRGKKSLTLNIAKPEGQRIVRELAKISDVLIEN
                     YKFGDLPRYGLGYEDLKKINPRLIYCSVTGFGHTGPYRERPGYDFMIQGMGGMMSVTG
                     EPDEAPGGGPQRAGVPVADIITGMYASIAICAALAHRAETGAGQHLDLALLDSQIALL
                     AYQNTNYFATGKPPKRIGNLHPNIVPYQPFRTKDGSVILACGNDNLYRKFCEAAGCAE
                     LADDSRFATNGKRVENRAEMTRLLAQVFAKRTTHEWVELLEAAGVPNGPINDVAQVFQ
                     EPQVVARGVKLELEHPVAGRLPTVASPMRFSATPVEHRLAPPVLGQHTDEVLRGLLAL
                     GDAEIARLRADGVI"
     gene            complement(10261..11046)
                     /locus_tag="VI319_14630"
     CDS             complement(10261..11046)
                     /locus_tag="VI319_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY6865132.1"
                     /translation="MTPGIRLSRDGHVATVALDNPGKLNAITLRMWEDLARVIGELSA
                     DDSLRCVVIRGAGEDAFAAGADIAEFARVRDNREQGKLYHRKYVYGALRAVADCRHPT
                     VAMIRGACAGGGLEIACQCDLRISGRSGRFGVPINRLGFAIAYDELASLLPLAGKAVV
                     LEILLEGRMLSADEAYAKGLLTRVVADEEVEAEAYATARRIADGAPLVARWHKQYVRR
                     LMPPAIPLSESELDENFAYFATEDYRIGQRAFLEKKKPGFVGR"
     gene            11150..11947
                     /locus_tag="VI319_14635"
     CDS             11150..11947
                     /locus_tag="VI319_14635"
                     /inference="COORDINATES: protein motif:HMM:NF023472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HEY6865133.1"
                     /translation="MASSASAARLAWLALACVVAACTPARTELSGLHSDDAREQRWAD
                     EIVPTLVVGSAVWLQPPGPQKKFLGLFTEPGQGAKAARGAIVLVHGLGVNPDYGVIGS
                     LRTSLADAGWTTLSIQMPVLAAGEPAERYPGLFTEGSDRIDAAVSWLRAKKYRRIVLL
                     SHSMGARMANHYLGRNPDAPIAAWIALGISSGEFEPGTAGRVPIFDIYAEHDLPAVLA
                     GAPERARVLSRVPGSRQAMVYGADHYFTRKEKELGTLIEMLLERPGA"
     gene            12079..13089
                     /gene="dctP"
                     /locus_tag="VI319_14640"
     CDS             12079..13089
                     /gene="dctP"
                     /locus_tag="VI319_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY6865134.1"
                     /translation="MRKLTTVALTAIVAGAFTALFANAALAAPTVLRASHQFPGGKGD
                     VRDEMVQIIARDAKAANVDLDIQVYPGASLFKPNEQWNALVNGQLDISSFPLDYASGK
                     VRAFGATLMPALVRSHDRAQRMGDSQFMKDIKAQIDRAGVVVLADAWLAGAVASKKAC
                     IKTPADIKGLKIRSAGPTFAAMWQAAGASIVSVPSNEVYNALQTGVADATDTSSGSFV
                     SFRIYEQVKCLTAPGENALWFMYEPVLMSKKTFNRLNKKQQDVIMAAGRKSQEYFAKE
                     AKKLDDDMVKVFKQHNVEVVTLTKGEYDQWVKVAQGSSYAEFAKEVPNGKKLIDEALA
                     VK"
     gene            13151..13711
                     /locus_tag="VI319_14645"
     CDS             13151..13711
                     /locus_tag="VI319_14645"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY6865135.1"
                     /translation="MQGYVRVMRAISQLCGFVAAGMIAAGVVVVCEMVFVRYVIGEST
                     IWQTDFTTYCLIAATFVGSPYVLLTRGHVNVNVLPLHLSPRGRYALALFAAIVSAAFC
                     AVMTVMTFLFWKEAWDNRWVSDTMWRARLWIPYSSMPIGLGLLTLQYASDIARLVSGR
                     DPPFDIPVGDDAAAVSANAAKAELTE"
     gene            13746..15062
                     /locus_tag="VI319_14650"
     CDS             13746..15062
                     /locus_tag="VI319_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY6865136.1"
                     /translation="MTPTHQGLIVLVVTLAMLMSGAPVAFGLGAISIVFVLLFQGLDA
                     MHVVAETFYSGLNDFTLVSIPMFVMMGAAIGSSPAGKDLYEALDRWLYRVPGGLVISN
                     LGACALFAALTGSSPACCAAIGKMGIPEMRRRGYPDDVATGSICAGGTLGILIPPSIT
                     FILYGIATETSIGRLFLAGVFPGLLLTGLFMLWTLFIIWKRGFRAHAADFRYSWRQKF
                     TSIPKIAPFLVIVVGVMYVLYGGVATPSEAAGVGAALCFVLAVIIYRMWRPAQWWQIL
                     RDTTRESVMILMIIAAAVLFGYMLTSLYLTQTLAQAIADMHVNKWVLMALINLLLLVC
                     GFFIPPAAIILMTSPILYPIIRAAGFDPVWFGVILTINMEIGLIHPPVGLNIYIVQAI
                     APDVPVTRVMWGTIPYVACMMLAIVILCVFPEIATWLPDRLMGSQH"
     gene            15226..15825
                     /locus_tag="VI319_14655"
     CDS             15226..15825
                     /locus_tag="VI319_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY6865137.1"
                     /translation="MFQVRIHGRGGQGVVTAAELLSVAAFDEGRHAQAFPSFGSERMG
                     APVMAFCRIDEREIRLREPVMNPDALIVQDPTLLHQVALFSGLAPSGYILINSSRSFD
                     ELGLGDFACGFPADHLCTVPASELALEHLGRALPNAPLLGGFAAICGQVGIDSVTTAI
                     RERFPGRTGELNAAAARAAYEQVARQRTQSQHDADHAHA"
     gene            15818..>16072
                     /gene="porA"
                     /locus_tag="VI319_14660"
     CDS             15818..>16072
                     /gene="porA"
                     /locus_tag="VI319_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEY6865138.1"
                     /translation="MLEQIEGSRAIARTIALCRPEVICAYPITPQTHIVEGLGEMVKS
                     GALEHCEFINVESEFAALSVAIGASAAGARSYTATASQGIL"
BASE COUNT         2256 a         5556 c         5706 g         2554 t
ORIGIN      
        1 gtgggcgggc tacgagtacg tgcgcgagat ggagcgcttc ctcctcgacg gacaggagca
       61 ggcgctgctc gcgaccgcgc gcgcgatcgc gaccgcgctg cacgaccggc ccggcctgat
      121 ggcgccgcgc gtcgagcgcg aggccgatcc gcgcgtgcgc gccgagcagg agctcgcgcg
      181 cctcgccgcg cagcgcggcg aggccggcgg cgccggcgac ccgccgaagc ccgacgccgc
      241 ggcgcccgac ggctcggcgc cgcccgcccc caccgccccg cggccgccgt cgatcatcgc
      301 ccgcgagtcc tcgccgcgcg acgagccgcg cgagatcgcg gacatcctgc gcggcgtgca
      361 gcacaccacc tcgcgcgtct gggtcgtgtc gcgcgacctg cgcgtgctcg cgctcgccgg
      421 cacgctgcgc cgtcccgagg gcgacgagaa cgaaggctgg gcgcaccggg tgctcggcga
      481 gctgttcccg cgcccgagcg agaacttcga cgacacgatc ggctacgacg cgctcgccaa
      541 cggccgcgag atcgccgcgg ccctgcgcgg cgtgcccgac gtcggcctgc gcggcacgcc
      601 cgacggacag gcggtggtga tgacggcggc gcacccggtg tggagcggcg acgatgtcgc
      661 gggcgcggtc gtcgccgagg agacgaccaa ctcgatcctc accgcgcgca gccgcgcgct
      721 cgagcggctg ctggtggtga cgctcgcggt gttcgcggtc gccgccgggg tgctgatctg
      781 gctcgcgacc ggcatctcct cgcgcatccg gcgcctgcgc gacgaggccg agacggcgat
      841 cgatgcgcgc ggccggctcg cgcacgtcgt caccgcgcag cgctcgggcg acgagattgg
      901 cgatctgtcg cgctcgttct cggcgctgct cggcaagctc gggcagtaca acgcctacct
      961 cgagggactg gcgagccgca tgtcgcacga gctgcgcacg ccgatcgccg tggtgcgctc
     1021 ctcgctcgag aacctgaagc tctcgcccac ggccgaggag gcgcgcgtct acatcggccg
     1081 cgccgaagac gggctgcggc ggctcggcac gatcctcacg cgcatgagcg aggccacgcg
     1141 cctggagcag ggcctgtcgt ccctggagcg cgagcgcttc gacctcgccg cggtggtgcg
     1201 cggctcggtc gacggctacc gcctcgcgta tcccgaccgg cgcttcgagc tgcacctgcc
     1261 gggcgtgccg gtgccggtct cgggatcggc cgacctggcg gcgcagctgc tcgacaagct
     1321 ggtcgcgaac gcggccgact tctcgacgag cggcgacccg atcctcgtgc gcctcgacgc
     1381 cgcgggcaat gcggcgcggc tgcgggtcga gaacagggga ccgctcctgc ccgagacgat
     1441 gcgggcgaag ctgttcgagg cgatggtctc ggtgcgcggc gatcgcgccg acaagcccgg
     1501 cgccgagccg cacctgggcc tcggcctcta catcgcgcgc gtgatcgccg agttccacgg
     1561 cggggcgatg gccgcggaga acctcgcgtc gggcgacggc gtcgccgtca ccgcgagctt
     1621 ccgcgtcgcg cccgagccga aggcgctcgc gcctacgcgt tgacgacggg ggccgagggc
     1681 tccgccgggc gcgccagccc gtgcttctcg atccgggagc gcagcgtgtc gcgcgtgatt
     1741 cccaggatgc gcgcggcctg cgtcacgttc cacgccgtgc gctcgagcgc ctggcgcaat
     1801 agatccctct cgacctgcgc cagctcgagc gtcacctgcg gcgcctcggg ctcgggcgcg
     1861 gcggcggccg agcaggtctc gacggcgggc agcccgagat cggaatagtc gatcgtcgcg
     1921 ccgcgcgcga gcagcaccgc ctgctcgatc aggttgcgca gctcgcgcac gttgcccggc
     1981 cagtcgtggc gcgtcaggcg gtcgagcgcg gcgggcgtaa acgcgagacc cggcttgccg
     2041 tagcgcttgg cgaactcgcc gaggaagcgg ttggcgagga gcaggatatc ctcgccgcgc
     2101 gcgcgcagcg gcggcacctt cagctcgatc acgcgcaggc ggtagagcag gtcgggccgg
     2161 aatttccctt cccgcgcgcg ctcctcgagc agctggttgg tcgccgcgat catgcgcacg
     2221 tcgacgcgcc gctcctgcac gctgccgacg cggcggatgc gctgctcctc gatcaccttg
     2281 agcagcttcg cctgcgtcga ggcgtcggcc tcgccgatct cgtcgaggaa cagcgtgccg
     2341 ccgtccgccg cctcgatcag gccgatcttg cgctcgttgg ccccggtgaa cgcgcccttc
     2401 tcgtagccga acagctcggc ctcgagcagg ttcgcgggga tcgccgcgca gttgatctcg
     2461 atgaacggcc cgtccttgcg ccggctctcg gagtggcagg cgcgcgcgat catctccttg
     2521 cccgagccgg tctcgcccgt gatcaggatc gcgggcgcgg ggccgccggt gtcgccggcc
     2581 tcggcgcgca gcaccaggcg gatgcgctcc ttcagctcga tcatcgcggg cgactcgccg
     2641 aggatctgcg acatgcagcc ctggtcgcgc gcgtggtagt acgcgagcgc cttgcgcatg
     2701 cgtccctcgc cggcgagctt gtcgagcacg cgcttcaggt cggcgagcac gagcggcttc
     2761 ggcaggaagt cggcggcgcc ggccttcatc gcgtcgaccg cgagctgcac gctgctctgg
     2821 ccggtgacca tgacgacgcg cgcgtcgggg tcgcgctcgc ggatcgtcgc gagcgcggcg
     2881 cgaccgtcca tgccgggcag ctggaagtcg agcaccacga cgtcgggctg cagcttcgcc
     2941 agctgcgcga gcccggactc ggccgtgccc gcgagctcga cgtcccagcc ctgccgcgcg
     3001 aggtagcgct ggacgttctt cgcgagggtg tcctcgtcct cgatcagcag cagcgacggc
     3061 atgtcagacg gccctcggca gctccagggt gacggtggtg ccggccccgg gcgcgctgtc
     3121 gatcttcacc tggccgccga agcgctcgac cgcgcgccgc acctgcgcca ggcccaggcc
     3181 gaagccgtgc ggcttggtcg tgtagaaggg gcggaacacg tgcggcaggt cggccggcgc
     3241 gatgcccttg ccggtgtcgc ggatgcggat ctgcacgcgg ccggcgccgt tgcgcgcgac
     3301 cttgccctcg atgcggccgc cgtcgggcgt cgcgtcgacc gcgttcgcga ccacgctgtg
     3361 cagcgcgtgc tcgagcagcg tcgagtcggc gtcgatgcgc gcgccctggc cctcgtcggc
     3421 gacggtcgcg acgaggttgc gccgctcgaa gtcgcgcgcc atgtcgttga agcacttgcg
     3481 cacgagagcc gcgacgtcca cgggcgcgcg ctggccgggc tcctgccgcg aaaagcgcag
     3541 cagctcgcgc atccaggcct cgacgcggtc gacctgggtc atgatgtcgc gcgcctgctc
     3601 ggtgcagtcc tcgcccttca tctcgagcgc gagctcggcc gaggtgcgga tcgaggcgag
     3661 cgggttgcgg atgttgtgcg cgaccgagga ggcgagctcg ccgacggtgg agagcgtctc
     3721 ggcctcgtgc agccgctcct gctgcctgcg gatcgtctcg ttgccgcggc gcacgaccca
     3781 gtagagcgtc acgtagagca cgagcgcgcc gagcatcgcc gccgcccaca cctgcttgcg
     3841 tccctcgatg atggccgcgg tcagctcgac cggcgccttg tagatctcga ccacgcccag
     3901 cacgcgcgtg ccttcggggt cgcgcaccgg gatgtaggtc tcgacgaaga agtcgctctc
     3961 gggatgcagg ccgacgtgct cgggcttctt gcgcagctcg tcggtgatgc ggccggagtt
     4021 gatgaccagc tcgcccttca gcgcgtcgtc gagctcgtcg ttctcgccga gctccttgcc
     4081 gatcagctcc ggatcgctcg accagagcat caccttgctg cgcgaataga cgttgttgcg
     4141 gatgacgtcg cgcatggcat tgaagtgatc gatcgtgccg cggaagcgct tggcgagcgc
     4201 cggatcgtcc gggtgatcga gataggcata cgagccgtcg gcgctcagca ggttctgcac
     4261 gatgtcgcgc gtgacgtggc cctcgcgctc gagcaggcgc ttggtgagga agtccgaaag
     4321 gactatggcg ttcagcacgg cgatgccggc gatcaccacc aggctcgcga gcgagaacca
     4381 gcgcagcagg ttgaaggggc gtttgagggg atcgaggggc atacgggaat tctgggcggc
     4441 gcggctgtgg ggcttttgac ccatctcaaa aaagatgggg tccgatgccc cgggggatat
     4501 cccccgggtg aaaggaccgc attatcccaa gtgcatgaaa gatcgtggat atagtttttt
     4561 gatgacgcag gcacggctcc tgcgacaggg agggcgtcgc ccctcgaggg cccgacttgc
     4621 ccacccagga gatgatcatg ctgaagtcga acaaagttcg tgtgctcgca atcgctgtcg
     4681 cgctgatggg cgcgatgggc gccgtcgcca tccccgccca ggcccggagc gcgaaatgct
     4741 tcgcgacccc ggtccccggc cagcccggcg tcgagatcgt gacctgcacg acgacccgtc
     4801 cgtaaggacc cgcaggaaca gagacccttc ctccccggtc tcggcggcgc cccggcggat
     4861 cccgccgggg cgccgttttt ttgctcgtgc gcgcgccttg cgggcggtca aaggcgaagc
     4921 gccgcgtccc ggcacactgg tcccatgcgc ctcctggtcc tcctcgccgc gctcgcggcc
     4981 cccctcgcgc acgccgtcac gccgggcgcg gcgcgcagcc agtcgcagga cgtcttcggc
     5041 gggaagctcg agaccggcct cacctacgag gtcacgcgcg cgaacgagat caagctcggc
     5101 gatgggccgg tgctgctgct ccccggagac cccgtccgaa aatcggatac ctaccggcac
     5161 ttcgcgggcg gcgcgaatct caacgcgcca tgcggcgcgc agctcaagtg cgtcgccaac
     5221 gcgagcgtcg actcgcgcac cggcgtgcgc tcgagcgagt tcgacacggc gatcgccgag
     5281 ggcgacgccg gcgtgcagta cacggccggc tcggtcgtct acggcctgcg cggcggccgc
     5341 cagcagtggg acgtcggcgg gcagtcgttc cgccgcgtga acaccctggt tgccgaggcg
     5401 gtgagcgcct tcagcaggca gctttcggcg tacggcgcgc tgaacgtctc gcgctacgag
     5461 cacccgggcg agaacgcctt cctcgacgcc gactaccgcg ccgtcaccgg caacctgcgc
     5521 tggggcgcgc aggaccgctg gcagtccgcc tacaccgcgc agctcacgct ctcgcgcgag
     5581 gacaaccggc agcaggatcc cacgctcgac gtgcgcggca cgctgatccg gctggcgtgg
     5641 gacgcgaagc cggcagcgcg ctgggccgtg cacccggcgc tcatgttcca gcgcctgcgc
     5701 ttcggcgacg tcgatccgct cttcggcgtg cgccgcgacg accgcttcac gcaggccgag
     5761 ctcgtcctca actacaggat cggcgagagc ctgcagatgc gcttcgagct gatgcgctcg
     5821 cgctaccgct cgagcgccgc ggtcatcgac aacgactggt cgagcgtcgg cgccggcgtg
     5881 caatgggagt tctagccgcg cgcttataat cggccgcggc gtggccaact acgacctgct
     5941 catcctcggc ggcggcatca acggcgccgg catcgcgcgc gacgccgccg gccgcggcct
     6001 caaggtgctg ctggtcgagc ggggcgacct cgcgcacgcg acctcctcgg cctcgaccaa
     6061 gctgatccac ggcgggctgc gctatctcga gtacttcgag ttccgcttgg tggcggaggc
     6121 gctcgcggag cgcgaggcgc tgctgaagat cgcgccgcac atcgtctggc cgctcgagtt
     6181 cgtgctgccg cacgagaagc acctgcggcc cgcgtggatg atccgcgccg ggctgttcct
     6241 ctacgatcat ctcggccacc gctatttcgg cggacgcagc tcgctgccgc attcgcgggc
     6301 ggtgccgctc gacgccgaag gcttcggcgc cggcctgaag cgcgagttcg cgcgcggctt
     6361 cgcgtaccac gactgctggg tcgacgacgc gcggctggtc gtgctgaacg cgcgctcggc
     6421 cgccgcgcac ggcgccgaga tcgccacgcg cacccgcctc gcgcacgccc ggcgcgaggg
     6481 ccgcgagtgg gtggcgcgcc tggagtcgcg ctcgggcggc cgcgaggtgc gcgccgccgc
     6541 gctggtgaac gcggcggggc cgtgggtgaa gcaggtgctc gcgggcgtgc tcggcgtcga
     6601 catcccggcg aaggtgcggc tggtgaaagg cagccacatc gtcgtgccgc gcatccacgc
     6661 gaagcgccac gcgttcatcc tgcaaaaccc ggaccggcgc gtgatcttcc tcattcccta
     6721 cgagcgcgag ttcaccctgg tcggcaccac cgacatcgcg gtcggcgatc acgacttcga
     6781 ggcgaagatc tcgccggagg agatcgagta tctgtgcgcg gcggcgagcc gctacaccga
     6841 gcgcccgatc gcgcccgctc aggtcgtgtg gagctactcg ggggtgcgcc cgctgtacga
     6901 cgacggcagc cgcgatccgt cggcggtgac gcgcgactac catctgctcg tggacgaggg
     6961 aagcgaaggg cgcgaggcgc cgctcgtgtc ggtcttcggc ggcaagatca cgacgtaccg
     7021 gcgtctcgcc gagcacgcga tggagaagct cggccggcgc ttcccgggca cgcgcgcctg
     7081 gacccgcacc gagccgctgc ccggcggcga cttccgcgac cgcagcttca ccgaagtgtt
     7141 cgacgattgc gtgcgccggc acccggggct gcccgcgccc tggctggcgc gcctgctgcg
     7201 gcgccacggc acgctcgccg acgcggtcat cggcgacgcg agctcgcgcc cgggtctcgg
     7261 cgaggatttc ggcggcgggc tggtccagcg cgaggtcgac tacctcgtcg cgcacgagtg
     7321 ggcgcgcgag gacgacgacg tgctgtggcg gcgcacgaag tgcggcctgc acatgagcgc
     7381 ggcgcagcgc tcgcgcgtgc gcgactatct ctcgagcatc ggctgagatg gaattgggat
     7441 ggtggctggg gtacctcgtc accggcgcgg tcgccggatt cttcaccggg atgctcggca
     7501 tcggcggcgg ctcgatcatc gtaccgatgc tggtcgcgct gttcggggcg caggacctgc
     7561 cgcgcgcgca cctcatgcac ctcgccgtgg gcaccgcgct cgcgaccttc ctgttcacct
     7621 cggtcgcgag cgtgcgcgcg cacgcgcagc gcggcgcgat ccgctgggac atcgccaggc
     7681 gcatgacccc gggcatcctc gccggcggcg tgatcgggtc gttcctctcg cgctacgtct
     7741 cgaccgcggg gttcgccgcg atcttcacgc tggtgatata tgccgcggcg accagcatgc
     7801 tgatcgacat caggccgcgg gcatcgcgcg atctgcccgg cacggccggg atgttcgcgg
     7861 ccgggttcct cttcagcggc atcgccgcat tcgcggccgt tggcggcgcc ttcatgacca
     7921 tccccttcat gatgtggtgc ggcgtgacgc tcatcgaggc gatcggcacc gcttcgctga
     7981 tcgggctgcc gatcgcgctc tcgggctcgg ttggcttcat gatcgcgggc ttcggcgagc
     8041 aggcgctccc cgcctggagc gtcgggtacg tctatctgcc ggcgctcgcc ggcgtcgtgg
     8101 tcgcgggcgt gctcgcggcg ccgctcggcg cggcggtcgc gcatcgcacg ccgacgcgct
     8161 ggctcaggcg cgtcttcgcc gtgctcttct ttgtcgtcgc gactcggatg ctgatcgccc
     8221 tgtggtgagc ctcgatcagg gccgctggaa caccaccagg tagttctccg agaggaaggt
     8281 ctcctcgtcg cgcaggcgga agccggcctc ctcgaagtcg cgcagcacgc tcgccttgtc
     8341 gaagcgcgcc ggcacgtgct cgaggatcca cgcgggcgag cgtcccggga cgcggtcgta
     8401 gtcgacgagc accaggcgtc cgcccggctt gagcgagcgg tagatggagt gcagcatcgt
     8461 ccacggcttg ccgaagtggt gatagacgtc gcacaggaag gcgacgtcga gcgaggccgg
     8521 cggcaggccg gtgtcggcct cgccgttcac gacggcggtc aggttgccga ggtgctcggc
     8581 gcgcgcgagg acggtcgtct tcagcaccgc ccagcgcgac acgtcggtcg cgtagacgcg
     8641 gccggtcggc ccgacgcgcc gcgcgaacag gcgcgcgaac agcccggtgc ccgcgccgat
     8701 gtcggcgacg gtcatgcccg gtctcaatcc gaccgcgtcg acgatccgca tgcgctggtc
     8761 gtagacctcg cggccgtcgt gctcgaggcc gctcttccac tccgagagca cgaagcccgg
     8821 gacgagcgcc gccgggctcc agctggacgt tctgcccgag attgcgacgg cggccgcgca
     8881 cacggcgacg gccaggacga cgggcccgac ccgcaaatgg gggggcccgt ggacgcgctt
     8941 catccatccc tccggccctc ccggactaga tggactcagc gtatacgtcg tcgtcgggcg
     9001 acgctatgac aggaccgcga tgcgaacgga cgaggttctt tcagatcacg ccgtcggcgc
     9061 gcaggcgggc gatctcggcg tcgccgagcg cgagcagtcc gcgcaaaacc tcgtcggtgt
     9121 gctggccgag caccggcggc gcgagccggt gctcgaccgg cgtcgccgag aagcgcatcg
     9181 ggctcgcgac cgtcggcagg cgcccggcga cgggatgctc gagctcgagc ttcacgcccc
     9241 gtgccacgac ctgcggctcc tggaacacct gcgcgacgtc gttgatcggt ccgttgggga
     9301 cgccggccgc ctccaggagc tccacccact cgtgcgtcgt gcgcttcgcg aacacctgcg
     9361 cgagcaaccg cgtcatctcg gcgcggttct cgacgcgctt gccgttggtc gcgaagcgcg
     9421 aatcgtctgc gagctcggcg cagcccgccg cctcgcagaa cttgcggtag agattgtcgt
     9481 tgccgcaggc gaggatgacg ctgccgtcct tcgtgcggaa cggctggtac ggaacgatgt
     9541 tcggatgcag gttgccgatc cgcttcggcg gcttgccggt cgcgaagtag ttcgtgttct
     9601 ggtacgcgag cagcgcgatc tgcgagtcga gcagcgcgag gtcgaggtgc tggccggcgc
     9661 cggtctcggc gcggtgcgcg agcgcggcgc agatcgcgat cgacgcgtac atgccggtga
     9721 tgatgtcggc gaccggcacg ccggcgcgct gcggcccgcc gcccggggct tcgtcgggct
     9781 cgccggtgac gctcatcatg ccgcccatgc cctggatcat gaagtcgtag ccggggcgct
     9841 cgcggtacgg gccggtgtgg ccgaagccgg tgaccgagca gtagatcagg cgcgggttga
     9901 ttttcttcag gtcctcgtag ccgaggccgt agcggggaag gtcgccgaac ttgtagttct
     9961 cgatcagcac gtcggaaatc ttcgccagct cgcgcacgat ccgctggccc tcgggcttgg
    10021 cgatgttgag cgtgagcgac ttcttgccgc ggttcgccga cgtgtagtac gcgctgtctt
    10081 tcgtgtcgcg gccggcggcg tccttcaccc agggcgggcc gaaggcgcgc gagtcgtcgc
    10141 ccgtcccggg gcgctcgacc ttgatcacct cggcgccgag gtcggcgagg ttctgcccgg
    10201 cccacgggcc cgcgagcacg cgcgagaggt cgagcacccg gatgtgcgag agcggtccca
    10261 tcagcgcccc acgaagccgg gcttcttctt ctcgaggaag gcgcgctggc cgatgcggta
    10321 gtcctcagtc gcgaagtagg cgaagttctc gtcgagctcg ctctccgaga gcgggatcgc
    10381 gggcggcatc aggcggcgca cgtactgctt gtgccagcgc gcgaccagcg gcgcgccgtc
    10441 ggcgatgcgg cgcgcggtgg cgtatgcctc ggcttcgacc tcctcgtcgg cgacgacgcg
    10501 ggtcagcagc cccttggcgt aggcctcgtc ggccgagagc atgcggccct cgagcaggat
    10561 ctcgagcacg acggccttcc ccgcgagcgg cagcagcgac gccagctcgt cgtacgcgat
    10621 cgcgaatccg aggcggttga tcggcacgcc gaagcggccc gagcggccgg agatgcgcag
    10681 atcgcactgg cacgcgatct cgaggccgcc gcccgcgcac gccccgcgga tcatcgcgac
    10741 ggtgggatgg cggcagtcgg cgaccgcgcg cagcgcgccg taaacgtatt tgcggtggta
    10801 gagcttcccc tgctcgcggt tgtcgcgcac gcgcgcgaac tcggcgatgt cggcgcccgc
    10861 ggcgaacgcg tcctcgcccg cgccgcggat cacgacgcag cgcagcgagt cgtcggcgga
    10921 gagctcgccg atgactcgcg cgagatcctc ccacatccgg agcgtgatcg cgttcagctt
    10981 gccgggattg tcgagcgcga ccgtcgcgac atgtccgtct cggctcaggc ggatcccggg
    11041 ggtcatggca ggcaggagcg gcgcgcggtg gaaagctgta gtttagctga aagctccgcg
    11101 gctcccggcg ggcgcggcta gaatgcggtg gcatccgctc gagggaccga tggcatcgtc
    11161 tgcatccgcc gcccgcctcg cctggctcgc gctcgcctgc gtcgtcgccg cgtgcacgcc
    11221 ggcgcgcacg gagctctccg gcctgcattc ggacgatgcg cgcgagcagc gctgggccga
    11281 cgagatcgtg ccgacgctcg tcgtcggcag cgccgtctgg ctgcagccgc cggggccgca
    11341 gaagaaattc ctcgggctgt tcaccgagcc cgggcaagga gcgaaggccg cgcgcggcgc
    11401 gatcgtgctc gtgcacggcc tgggcgtgaa tcccgactat ggcgtgatcg gcagcctgcg
    11461 cacgtcgctc gccgacgcgg gctggaccac gctctcgatc cagatgccgg tgctcgccgc
    11521 gggggagccc gccgagcgct atcccgggct gttcacggaa gggtccgacc gtatcgacgc
    11581 ggcggtgtcg tggctgcgcg cgaagaaata ccggcgcatc gtcctgctct cgcacagcat
    11641 gggcgcgcgc atggcgaacc actatctggg gcgcaacccc gacgcgccga tcgccgcctg
    11701 gatcgcgctc gggatctcga gcggcgagtt cgagccgggc acggccggac gcgtgccgat
    11761 cttcgacatc tacgccgagc acgatctgcc cgccgtcctc gccggggcgc ccgagcgcgc
    11821 gcgggtgctc tcgcgcgtgc cggggtcccg tcaggcgatg gtgtacggcg ccgatcacta
    11881 cttcacccgc aaggagaagg agctcggcac gctgatcgag atgctgctgg agcgtcccgg
    11941 ggcctgagag cccggatttg gtccgcgggc ggaaaggtgc ttagaatacc ggccggagga
    12001 attctggcgg atgccccacc cgcgtgcagg cacaaagcgg gcggaccgcc gttcatcgat
    12061 acccggagga ggtctgcaat gagaaagctc actaccgtcg cgctgacggc catcgtcgcc
    12121 ggcgctttca cagccctatt tgcgaacgcg gcgctcgccg cgccgaccgt gctgcgcgcg
    12181 tcgcaccagt ttcccggcgg taagggcgac gtgcgcgacg agatggtcca gatcatcgcg
    12241 cgcgacgcca aggccgccaa cgtcgacctg gacatccagg tctatcccgg cgcgtcgctg
    12301 ttcaagccga acgagcaatg gaacgccctg gtcaacgggc agctcgacat ctcgtcgttc
    12361 ccgctcgact acgcgagcgg caaggtgcgc gccttcggcg cgacgctgat gccggcgctg
    12421 gtgcgcagcc acgatcgggc gcagcgcatg ggcgactcgc agttcatgaa ggacatcaag
    12481 gcgcagatcg accgcgccgg cgtcgtcgtg ctggccgacg cctggctcgc gggcgcggtc
    12541 gcgtcgaaga aggcctgcat caagacgccc gccgacatca agggcctgaa gatccgctcc
    12601 gcgggcccca cgttcgcggc catgtggcag gcggccggcg cctcgatcgt ctcggtgccc
    12661 tcgaacgagg tctacaacgc gctgcagacc ggcgtcgccg acgcgaccga cacgagctcg
    12721 ggcagcttcg tctcgttccg catctacgag caggtgaagt gcctcaccgc tccgggcgag
    12781 aacgcgctct ggttcatgta cgagccggtg ctgatgtcga agaagacgtt caaccgcctg
    12841 aacaagaagc agcaggacgt gatcatggcg gccggccgca agtcgcagga gtacttcgcc
    12901 aaggaagcga agaagctcga cgacgacatg gtcaaggtgt tcaagcagca caacgtcgag
    12961 gtcgtgaccc tgacgaaggg cgagtacgac cagtgggtca aggtcgcaca aggcagctcg
    13021 tacgccgagt tcgccaagga agtgccgaac ggcaagaagc tgatcgacga ggcgctcgcg
    13081 gtcaaatagg accgcggacg ggaagggcgg cgcgagccgc cctttttttc atccgcgagg
    13141 gaggcagccg atgcaaggct acgtgcgcgt catgcgcgcg atctcgcagc tgtgcggttt
    13201 cgtcgccgcc ggcatgatcg cggcgggcgt ggtcgtcgtg tgcgaaatgg tgttcgtgcg
    13261 ctacgtcatc ggcgagagca cgatctggca gaccgatttc acgacctact gcctgatcgc
    13321 cgcgacgttc gtcggcagcc cctacgtgct gctgacgcgc ggccacgtca acgtcaacgt
    13381 gctgccgctg cacctgtcgc cgcgcggccg ctatgcgctc gcgctgttcg cggccatcgt
    13441 ttcggctgcg ttctgcgccg tcatgacggt gatgacgttc ctgttctgga aagaggcatg
    13501 ggacaaccgc tgggtctccg acacgatgtg gcgggcgcgg ctgtggattc cttattcgtc
    13561 gatgccgatc ggcctcggcc tgctcacgct gcaatacgcg agcgacatcg cgcggctcgt
    13621 gagcggccgc gatccgccgt tcgacatacc ggtgggtgac gatgcggcgg ccgtttcggc
    13681 gaacgccgcg aaggcggagc tgacggaatg acgacggcgt ttcgtccggt cggcgaggtg
    13741 actcgatgac gccgacccat cagggcctga tcgtcctggt cgtgactctc gcgatgctga
    13801 tgtcgggtgc gcccgtcgcg ttcggcctcg gcgcgatctc gatcgttttc gtgctgctct
    13861 ttcagggcct cgacgcgatg cacgtcgtcg cggaaacgtt ctattcagga ctgaacgatt
    13921 tcacgctggt ttcgatcccg atgttcgtga tgatgggcgc ggcgatcggc tcgtcgccgg
    13981 ccggcaagga tctctacgag gcgctcgatc gatggctgta tcgcgtgccc ggcggcctcg
    14041 tgatctcgaa cctcggcgcc tgcgcgctgt tcgcggcgct gaccggctcg tcgcccgcgt
    14101 gctgcgcagc gatcgggaag atgggcattc ccgagatgcg ccgccgcggc tatcccgacg
    14161 acgtcgcgac cggatcgatc tgcgccggcg gcacgctcgg catcctgatc ccgccctcga
    14221 tcacgttcat tctctacggc atcgcgaccg aaacgtcgat cggccggctg tttctcgcgg
    14281 gcgtgttccc gggcctgctg ctcaccgggc tcttcatgct ctggacgctg ttcatcatct
    14341 ggaagcgcgg ctttcgcgcg catgccgcgg attttcgtta ttcgtggcgc cagaaattca
    14401 cgtcgatccc gaagatcgcg ccgtttctgg tcatcgtcgt cggcgtgatg tacgtgctct
    14461 acggtggcgt cgcgacgccg tccgaggcgg cgggcgtggg cgctgcgctg tgcttcgtac
    14521 tcgccgtcat catctatcgg atgtggcggc ctgcgcaatg gtggcagatc ctgcgcgaca
    14581 cgacccgcga atccgtgatg atcctgatga tcatcgccgc cgccgtgctt ttcggctaca
    14641 tgctgacgtc gctgtacctg acgcagacgc tcgcccaggc gatcgccgac atgcacgtga
    14701 acaaatgggt gctgatggcg ttgatcaacc tgctgctgct ggtatgcggc tttttcatcc
    14761 cgccggcggc gatcatcctg atgacgagcc ccattctgta tccgatcatc cgcgccgcgg
    14821 gcttcgaccc ggtctggttc ggggtgatcc tgacgatcaa catggagatc gggctcatcc
    14881 atccgccggt cgggctcaac atctacatcg ttcaggcgat cgcgcccgac gtgccggtga
    14941 cgcgcgtcat gtggggaacc attccctatg tcgcctgcat gatgctcgcg atcgtgatcc
    15001 tgtgcgtctt cccggagatc gcgacgtggc tgcccgaccg gctgatggga tcgcagcatt
    15061 gaggcgtgat cgcgcgcacg ctgccgcgcg cgactcggca agattgccgc agctctgacg
    15121 tagcgctttg tccctgtgct ggtgctgcgg aattcgtccc tcgtttgacg agggtcaaca
    15181 tgcgcggcca gcgccgacgg gaacattcgg atcgggaggg agccgatgtt ccaggtgcgc
    15241 atccacgggc gcggcggtca gggcgtcgtc accgctgcgg aactgctgtc cgtcgcggcg
    15301 ttcgacgagg gacgtcatgc gcaggcgttt ccgagcttcg gctcggaacg catgggcgcg
    15361 ccggtcatgg cgttctgccg gatcgatgag cgcgagatcc ggctgcgcga gccggtgatg
    15421 aatcccgatg cgctgatcgt ccaagatccg acgctcctgc accaggtcgc actgttcagc
    15481 ggactcgcgc cctcgggcta catcctcatc aattcgagcc gcagcttcga cgagcttggc
    15541 ctcggcgact tcgcgtgcgg atttcccgcc gatcatttgt gcacggtgcc ggcgagcgag
    15601 ctcgcgctcg agcatctcgg acgtgcgttg ccgaatgcgc ccctgctcgg cggatttgcc
    15661 gcgatctgcg ggcaggtcgg catcgactcg gtgacgacgg ccatccgcga gcgcttcccc
    15721 ggccgcacgg gcgagctcaa cgcggcggcg gcgcgggccg cttacgagca ggtcgcacgg
    15781 cagcgcacgc agtcgcaaca cgacgcggac cacgcgcatg cttgagcaga tcgaaggctc
    15841 gcgcgcgatc gccagaacga tcgcgctctg ccggccggag gtgatctgcg cctatccgat
    15901 cacgccgcag acgcatatcg tcgagggact gggggagatg gtgaagagcg gtgcgctcga
    15961 gcactgcgag ttcatcaacg tcgaatcgga attcgccgcg ctttcggtgg cgatcggcgc
    16021 atcagcggcc ggcgcgcgca gctacacggc caccgcgagc cagggcatcc tg
//
LOCUS       DATDMW010000349         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1222092, whole genome shotgun sequence.
ACCESSION   DATDMW010000349 DATDMW010000000
VERSION     DATDMW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222092"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /gene="tsaB"
                     /locus_tag="VI319_14665"
     CDS             complement(<1..686)
                     /gene="tsaB"
                     /locus_tag="VI319_14665"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY6865139.1"
                     /translation="MNLLAIETSADFCSVAAARGDAAFSRHERAGQRHGELAIPMIDA
                     VLAEAGLALADLDGIAYGAGPGAFTGLRIACGVTQGLALARGLPVVGVGTLLALAEEA
                     AGDAAEAAVVACIDARMREVYHAAYRRARGEWREIAAPGLHRPDAVPLPDGEDWTGAG
                     SGFGAYPEALERRHAGRLGDIRPDVAPTARAVLRLAAREFAAGRGADAATAVPVYLRD
                     KVALKTSERA"
     gene            745..1722
                     /locus_tag="VI319_14670"
     CDS             745..1722
                     /locus_tag="VI319_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865140.1"
                     /translation="MRLAILGAAAVALGASVAFAQEQYPSSRPITMVVAFPPGGVADI
                     TGRPTAIALERVLKQRVIVENRPGAAGATGNAYVAHAKPDGYTILMALSSISVIPEAE
                     RLQGHKPPYELSELAPIALISADPVVLVVREDAPWKSVKDFVADAKQRPGKISYSSSG
                     IFGALHMPFALLENATGTTLWHVPYNGGGPAVQALLGGQVDSTVGGPAAMIGQIRAKK
                     FRPLASFGGKRLASLPDVPTLKELGIDAEYYIWAGIMVPAATPAAVQQKLREGVRQAV
                     NDPDFRNAMTKVETPISYLDAPEFRKYWDADAHKLAAAVKTVKIEEQKK"
     gene            1724..2659
                     /locus_tag="VI319_14675"
     CDS             1724..2659
                     /locus_tag="VI319_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY6865141.1"
                     /translation="MAAAQGKVIVTCAVTGSIHTPSMSPYLPVTPDEIAEAAIGAAKA
                     GAAIVHLHARDPKDGRPTQDPKVFMQFLPKIKAASNVVVNLTTGGAPTMSTEERLQPA
                     LQLQPEVASLNMGSMNFGLYEMIGRYKEWKHDWEKPYLAGSDERIFKNTFRDIAYILE
                     SCSGNDTRFEIECYDIGHLYTAAHFLDRRLVKPPLFIQSVFGIRGGIGPHPEDVLHMK
                     RTADRLFGDQYLWSVLGAGRNQMFVAAQSAVMGGNVRVGLEDSLWLGRGTLAKTNAEQ
                     VTKIRKILEELGLAIATPDDAREMLKLKGGRNVAF"
BASE COUNT          436 a          952 c          939 g          363 t
ORIGIN      
        1 catgcgcgct cgctcgtctt caaagccacc ttgtctcgca ggtagacggg caccgcggtc
       61 gccgcgtcgg cgccgcggcc cgccgcgaac tcgcgcgccg cgagccgcag caccgcgcgc
      121 gccgtcggcg cgacgtcggg ccggatatcg ccgagccgcc cggcgtgacg ccgctcgagc
      181 gcctcgggat acgcgccgaa gccgctgccc gcgccggtcc agtcctcgcc gtcgggcagc
      241 ggaacggcgt ccgggcggtg cagccccggc gcggcgatct cgcgccattc gccgcgcgcc
      301 cggcggtacg cggcatgata cacctcgcgc atgcgcgcat cgatgcaggc gacgacggcg
      361 gcctcggcag catcgcccgc cgcctcctcc gcaagagcca gcagcgtgcc gacgccgacc
      421 accggcaggc cgcgcgcgag cgcgagcccc tgcgtgacgc cgcaagcgat gcgcaggccc
      481 gtgaacgcgc ccggtcccgc gccgtaggcg atgccgtcga ggtcggcgag cgcgagcccg
      541 gcttcggcga gcacggcgtc gatcatcggg atcgcgagct cgccgtggcg ctgccccgcg
      601 cgctcgtggc gggagaacgc ggcgtcgccg cgcgcggcgg cgaccgagca gaagtccgcg
      661 gaggtctcga tggcgagaag gttcatgccg agagcgcgat tctagcgcta tgattgcgcc
      721 tcacgcagtt ccggaggagg agtcatgcgt ctcgcaattc tgggcgccgc cgctgtcgct
      781 ctcggggcgt cggtcgcttt cgcacaagaa caatatccgt cgtcgcgccc gatcacgatg
      841 gtggtcgcgt tcccgccggg gggcgtcgcc gacatcaccg ggcggccgac cgcgatcgcg
      901 ctggagcgcg tgctcaagca gcgcgtcatc gtcgagaacc gtcccggtgc cgcgggcgcg
      961 accggcaacg cctacgtcgc gcacgcgaag cccgacggct acaccatcct catggcgctg
     1021 tcgtcgatct cggtgatccc ggaggccgag cggctgcagg ggcacaagcc gccttacgaa
     1081 ttgagcgagc tcgcgccgat cgcgctgatc tcggccgacc ccgtggtgct cgtggtgcgc
     1141 gaggacgctc cgtggaagag cgtgaaggac ttcgtcgccg acgcgaagca gcgcccgggc
     1201 aagatcagct acagctcctc gggcatcttc ggcgcgctgc acatgccctt cgcgctgctc
     1261 gagaacgcca ccggcacgac gctgtggcac gtgccgtaca acggcggcgg gccggcggtg
     1321 caggcgctgc tcggcggcca ggtcgactcg accgtcggcg gtccggcggc gatgatcggg
     1381 cagatccggg cgaagaagtt ccgcccgctc gcgagcttcg gcggcaagcg gctcgcctcg
     1441 ctgcccgacg tcccgacgct gaaggagctc ggcatcgacg ccgagtacta catctgggcc
     1501 gggatcatgg tgcccgcggc gacgccggcc gcggtccagc agaagctgcg cgagggcgtg
     1561 cggcaggccg tgaacgatcc cgatttccgg aacgccatga ccaaggtcga gacgccgatc
     1621 agctacctgg acgcgccgga gttcaggaag tactgggacg ccgacgcgca caagctcgcc
     1681 gcggcggtga agaccgtcaa gatcgaagag cagaagaagt agcatggccg ccgcgcaagg
     1741 caaggtcatc gtcacctgcg cggtgacggg gtcgatccac accccgtcga tgtcgccgta
     1801 cctcccggtg acgcccgacg agattgccga ggccgcgatc ggcgcggcca aggccggcgc
     1861 cgcgatcgtg cacctgcatg cgcgcgatcc gaaggacggg cggccgacgc aggacccgaa
     1921 ggtcttcatg cagttcctgc cgaagatcaa ggccgcgtcc aatgtcgtcg tcaacctcac
     1981 gaccggcggc gcgccgacca tgagcaccga ggagcggctg cagcccgcgc tgcagctcca
     2041 gcccgaggtg gcctcgctca acatgggctc gatgaacttc gggctctacg agatgatcgg
     2101 ccgctacaag gagtggaagc acgactggga gaagccctac ctcgccggct ccgacgagcg
     2161 catcttcaag aacaccttcc gcgacatcgc ctatatcctc gagtcctgca gcggcaacga
     2221 cacgcgcttc gagatcgagt gctacgacat cgggcacctg tatacggcgg cgcacttcct
     2281 cgaccgcagg ctggtcaagc cgccgctctt catccagtcg gtcttcggca tccgcggcgg
     2341 catcggcccc caccccgagg acgtgctgca catgaagcgc accgccgacc ggctgttcgg
     2401 cgaccagtac ctgtggtcgg tgctcggcgc gggacgcaac cagatgttcg tcgcggcgca
     2461 gtcggcggtg atgggcggga acgtgcgcgt cgggctcgag gacagcctgt ggctcgggcg
     2521 cggcactctc gcgaaaacga acgccgagca ggtcacgaag atccggaaaa tcctggaaga
     2581 gctcgggctc gcgatcgcga cgcccgacga cgcccgcgag atgctgaagc tcaagggcgg
     2641 ccgcaacgtc gcgttctgat gccgagcact ctcgaggagc tgtccgcgat
//
LOCUS       DATDMW010000350         4510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_724583, whole genome shotgun sequence.
ACCESSION   DATDMW010000350 DATDMW010000000
VERSION     DATDMW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724583"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VI319_14680"
     CDS             complement(<1..972)
                     /locus_tag="VI319_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY6865142.1"
                     /translation="MGIMPRNPADFDARHRGARSFVPGRFEKDAREAFDDGWTPEERD
                     ATQLKTTVTEERSRSILSHNDSPDVGFSTSLNPYRGCEHGCIYCYARPSHAYLELSPG
                     LDFESKLFAKTNAAELLRAALAKPGYVATPIALGANTDCYQPIERRYRITRQILEVLA
                     ECEHPVTMVTKSALVERDLDLLAPMAEKNLVKVFVSIGTLDRALAAKLEPRAATPERR
                     LAILGSLAAAGVPCGVMIAALIPALNDRTMEQVLEAAAAAGAREAAYVIMRLPHELKD
                     LFREWLAAHYPERAEHVISIVRQLRGGRDNDPRFGTRMTGIGSYAELI"
     gene            1065..2069
                     /locus_tag="VI319_14685"
     CDS             1065..2069
                     /locus_tag="VI319_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865143.1"
                     /translation="MTRIWVALAVAAAATAAYAQAPEKKDVHVAVGGKSSLYYLPLTI
                     AEQLGYFKAEGLNVQISDFAGGSQALRAVVGGSADVVSGAYEHTLNMQPKGQYLQCFV
                     QQGRAPQIAIGIAKSKAYKSPKDLKGLKVGVSAPGSSTHMIFNHLLSTEGLKPSDVSI
                     IGVGLGAAAVTALKNGQIDAISNTDPVMTKLEQDGDVRIIADTRTMKGTERVLGGPMP
                     AGCLYAPVDFIKKNPNTVQALTNAIVRADKWIQNASPDEVLRTVPESYTLGDKALYEA
                     SYAKVKEAISPDGYISPEGAKTALKVIPSFNPEVKASEIKLDLTYTNDFVKKANAKYK
                     "
     gene            2102..2908
                     /locus_tag="VI319_14690"
     CDS             2102..2908
                     /locus_tag="VI319_14690"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6865144.1"
                     /translation="MTPALALENVTCTFASRDDASQRYTAVRDTSLVVRAGEFVSGVG
                     PTGCGKSTLLNVAAGLLAPSAGEVRVHGAPLAGVNARAGYMFQSEALMPWRNTLANVT
                     AGLEFRGVARAEAQARGREWLERVGLAGFGDRYPHQLSGGMRKRVALAQMLILDPQIL
                     LMDEPFSALDIQTRQLMENELLELWSANRRSVVFITHDLEEAIALSDRVVVLSAGPAS
                     RPIGEFAVDLPRPRDVADIRLTPRFIELHTRIWHAMKEEVLKGYVRQQKH"
     gene            2886..3719
                     /locus_tag="VI319_14695"
     CDS             2886..3719
                     /locus_tag="VI319_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6865145.1"
                     /translation="MSGSRSTDARDRLRLRVIQAVLLLALLALWYVLTSPTLLPPFYF
                     NDPKQAAFLFGEPVQVAVRLWKWFASGEIYVHLWVTLVETLLAFLIGTVLGVIFGMWL
                     ALSPTSSAIFDPYITALNAMPRVILAPIFFIWFGLDIGSKVALGVTLVFFIVFFNVYQ
                     GVREVSPVVLANARMLGATRRQLLRHVYLPSAMSWVFASLHNSVGLAFVGAVVGEYLG
                     SARGVGYLILQAEGVFDMNTVVAGVLLLTVFALILDGLVGLAERRLMVWQPRSGETER
                     V"
     gene            3802..3878
                     /locus_tag="VI319_14700"
     tRNA            3802..3878
                     /locus_tag="VI319_14700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3836..3838,aa:Met,seq:cat)
     gene            4155..>4510
                     /locus_tag="VI319_14705"
     rRNA            4155..>4510
                     /locus_tag="VI319_14705"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          774 a         1409 c         1571 g          756 t
ORIGIN      
        1 gatcagctcc gcgtagctgc cgatcccggt catgcgcgtg ccgaagcgcg gatcgttgtc
       61 ccggccgccg cgcagctgcc gcacgatgct gatgacgtgc tcggcgcgct cggggtagtg
      121 cgcggcgagc cattccctga acagatcctt cagctcgtgc ggcaggcgca tgatcacgta
      181 cgcggcttcg cgcgcgcccg ccgcggcggc cgcctcgagc acctgctcca tcgtgcggtc
      241 gttgagcgcc gggatgagcg cggcgatcat cacgccgcag gggacgccgg cagcggcgag
      301 cgagcccagg atcgcgaggc ggcgctccgg tgtcgccgcg cgcggctcga gcttcgccgc
      361 gagcgcgcgg tcgagcgttc cgatcgacac gaacaccttc accaggttct tctcggccat
      421 cggcgcgagc aggtcgaggt cgcgctcgac gagcgccgac ttcgtcacca tcgtcaccgg
      481 gtgctcgcac tcggcgagca cctcgaggat ctggcgcgtg atgcgatagc ggcgctcgat
      541 cggctggtag cagtcggtgt tcgcgccgag ggcgatgggg gtcgcgacgt agcccggctt
      601 ggcgagcgcg gcgcgcagca gctccgcagc gttcgtcttg gcgaacagct tgctctcgaa
      661 atcgaggccg ggggacagct cgaggtaggc gtggctcggc cgcgcgtagc agtagatgca
      721 gccgtgctcg cagccgcggt aggggttgag cgaggtggag aagcccacgt cgggcgagtc
      781 gttgtgcgag aggatcgagc gcgagcgctc ctcggtgacc gtggtcttca gctgcgtcgc
      841 gtcgcgctct tcgggcgtcc agccgtcgtc gaaggcctcg cgcgcatcct tctcgaagcg
      901 gcccgggacg aaggagcgcg cgccgcggtg gcgtgcgtcg aaatcggcgg ggttgcgggg
      961 catgatcccc atggtacgcc gtgactggat gggtgtacag tacgccggtc ggcggcagcg
     1021 ccgtgtaata tggccactcg tccgacctca cgaggaggaa agccatgaca cgaatttggg
     1081 tcgcgctggc cgttgcggcg gccgcgacgg cagcgtacgc gcaggcgcct gagaagaagg
     1141 acgtgcacgt ggcggtgggc ggcaagtcgt cgctgtacta cctgccgctc acgatcgccg
     1201 agcagctcgg ctacttcaag gccgaggggc tgaacgtgca gatcagcgac ttcgccggcg
     1261 gctcgcaggc gctgcgcgcg gtggtgggcg gcagcgcgga cgtcgtgtcg ggggcgtacg
     1321 agcacacgct gaacatgcag cccaagggcc agtacctgca gtgcttcgtg cagcaggggc
     1381 gggcgccgca gatcgcgatc ggcatcgcca agagcaaggc gtacaagtcg ccgaaggacc
     1441 tgaagggact gaaggtcggc gtgtcggcgc cgggatcgag cacgcacatg atcttcaacc
     1501 acctgctgtc gaccgaaggg ctgaagccct cggacgtgtc gatcatcggc gtcggactcg
     1561 gcgccgccgc ggtgacggcg ctgaagaacg gccagatcga cgccatctcg aacaccgacc
     1621 cggtgatgac caagctcgag caggacggag acgtgaggat catcgccgac acgcgcacca
     1681 tgaaaggcac cgagcgcgtg ctcggcgggc cgatgcccgc gggctgcctg tacgcgccgg
     1741 tcgacttcat caagaagaac ccgaacacgg tccaggcgct gaccaacgcc atcgtgcgcg
     1801 ccgacaagtg gatccagaac gcgtcgcccg acgaagtgct gcgcaccgtg cccgagtcct
     1861 atacgctcgg cgacaaggcg ctgtacgagg cgtcctacgc caaggtcaag gaggcgatct
     1921 cgcccgacgg ctacatctcg cccgaaggcg cgaagacggc gctcaaggtc atcccgtcgt
     1981 tcaaccccga ggtgaaggcg agcgagatca agctcgacct gacttacacg aacgacttcg
     2041 ttaagaaggc gaacgcgaag tacaagtgac cgcgcgcacg cgcgggagtg acgatgccgc
     2101 catgactccc gcactggccc tggagaacgt cacctgcact ttcgcatcgc gcgacgacgc
     2161 gtcgcagcgc tacaccgccg tgcgcgacac gagcctcgtg gtccgcgccg gcgagttcgt
     2221 ctccggggtg gggccgacgg gctgcggcaa gtcgacgctg ctcaacgtcg ccgccgggct
     2281 gctcgcgccg tccgccggag aagtcagggt gcacggagcc ccgctcgccg gcgtcaacgc
     2341 gcgcgccggc tacatgttcc agagcgaggc gctcatgccc tggcgcaaca cgctcgcgaa
     2401 cgtcaccgcg gggctcgagt tccgcggcgt cgcgcgcgcg gaggcgcagg cgcgcgggcg
     2461 cgaatggctc gagcgcgtcg ggctcgccgg cttcggcgac cgctacccgc accagctctc
     2521 gggcgggatg cgcaagcgcg tcgcgctcgc gcagatgctg atcctcgacc cgcagatcct
     2581 gctcatggac gagccgttct cggcgctcga catccagacg cgccagctca tggagaacga
     2641 gctgctcgag ctgtggtcgg cgaaccgcag gtcggtggtc ttcatcacgc acgacctgga
     2701 ggaggcgatc gcgctgtctg accgcgtggt cgtcctctcc gccgggccgg cgtcgcggcc
     2761 gatcggcgaa ttcgcggtcg acctgccgcg gcctcgagac gtcgccgaca tccgcctgac
     2821 gccgcgcttc atcgagctgc acacgcgcat ctggcacgcg atgaaggaag aagtgctgaa
     2881 gggttatgtc cggcagcaga agcactgacg cgcgcgaccg gctgcggctg cgcgtgatcc
     2941 aggcggtgct gctgctcgcg ctgctcgcgc tttggtacgt gctcacgagc ccgacgctgc
     3001 tgccgccgtt ctatttcaac gatcccaagc aggcggcgtt cctgttcggc gagcccgtgc
     3061 aggttgcggt gcgcctgtgg aaatggttcg cgtcgggcga gatctacgtc cacctctggg
     3121 tgacactggt cgagacgctg ctcgcgttcc tgatcggcac cgtgctcggg gtgatcttcg
     3181 gcatgtggct cgcgctgtcg ccgacgtcgt cggcgatctt cgacccgtac atcaccgcgc
     3241 tgaacgcgat gccgcgcgtg atcctcgcgc cgatcttttt catctggttc ggcctcgata
     3301 tcggctcgaa ggtcgcgctc ggcgtgacgc tcgtgttctt catcgtgttc ttcaacgtct
     3361 accagggcgt gcgcgaggtg agcccggtgg tcctcgccaa tgcgcggatg ctcggggcga
     3421 cgcgtcgaca attgctccgg cacgtctatc tgccgagcgc gatgagctgg gtgttcgcga
     3481 gcctgcacaa ttcggtgggg ctcgccttcg tgggggcggt cgtgggcgaa tatctcggtt
     3541 ccgcgcgagg cgtcggctac ctcatcctcc aggcggaggg cgtgttcgac atgaacacgg
     3601 tcgtcgcggg agtcttgctc ctgactgttt tcgcgcttat attggacggg ttggtgggat
     3661 tggccgagcg gcgactgatg gtctggcagc cgcgatcggg cgagaccgag cgagtctgaa
     3721 ggaaaacccc tgttgcgacg cgtcaaaagg ttgacggtag tccgctctgg tcgctataat
     3781 cccgcttctg tacgacacgg gcgcggggtg gagcagtctg gcagctcgtc gggctcataa
     3841 cccgaaggtc ataggttcaa atcctatccc cgcaaccagc ctttcgaggc gggtctccgg
     3901 gtcagagtag tacagatcgt tctttaacaa atcgaacagc cgataggtgt gggcgctggg
     3961 tgaagccagt ggcgttccgc atgcgcaagc gtgcgggcga cacagacata cacttaggct
     4021 cacgccgagg tctagcaagg ttttcaacaa cctttgcaat gactttgagt gagctgcggg
     4081 ccggtggctt ccgagtcgca agacgaggga gcgaaggtcc gcacaatcgc aatgcgattg
     4141 atcaggtcaa atttgaattg aagagtttga tcctggctca gattgaacgc tggcggcatg
     4201 ccttacacat gcaagtcgaa cgcgaaaggg ggcaaccccg agtagagtgg cgaacgggtg
     4261 agtaatgcat cggaacattt cctgtagcgg gggacaacct cgagaaatcg aggctaatac
     4321 cgcatacgat cttcggatga aagtggggga ccgcaaggcc tcacactata ggagtggccg
     4381 atgccggatt agctagttgg cggggtaaaa gcccaccaag gcgacgatcc gtagctggtc
     4441 tgagaggacg accagccaca ctgggactga gacacggccc agactcctac gggaggcagc
     4501 agtggggaat
//
LOCUS       DATDMW010000351         3361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1472236, whole genome shotgun sequence.
ACCESSION   DATDMW010000351 DATDMW010000000
VERSION     DATDMW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472236"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VI319_14710"
     CDS             <1..217
                     /locus_tag="VI319_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY6865146.1"
                     /translation="TQVASRCGAEVWLSREGRRVNAKSIMGVMMLAAGKGSRVLVEAE
                     GADADDAMAAILALIAAGFGEEESRER"
     gene            214..1974
                     /gene="ptsP"
                     /locus_tag="VI319_14715"
     CDS             214..1974
                     /gene="ptsP"
                     /locus_tag="VI319_14715"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY6865147.1"
                     /translation="MNRRDTQTQSASFTLHGARVGEGIAIGFAHLFSTARLEVAHYQV
                     TDDDVEAELYRLDRAFEQVRGELRALQDGLPPGTPPEMGAFLRLHAMILDDANLAVAP
                     RELVRRHRYNAERALVQQMDELVAQFERMEDAYLRERKQDVVQVVERVLKAMQGTGHA
                     PAAPAHEENVIVVAHDLSPADMMIFKQHQYAGFVTDLGGATSHTAIVARSLAIPAIVG
                     LRHARATIRENDLLIVDGTHGVLIVNPGGLVLEEYRRRQGALEEERRKLKGLRSTRAS
                     TLDGARIALQANIELPKDVGEAQEVGAGGIGLFRTEFLFLNRDDLPSEDEQFEAYRAV
                     AQAMAGRPVTIRSLDLGADKSINGSRAGPNPALGLRAIRFCLAEPQIFLAQLRAILRA
                     SHYGRVRLLVPMLAHAHEVEQALQAVRQAKAGLDEERVPYDAALKVGGMIEVPAAALS
                     LKYLISKLDFLSIGTNDLAQYTLAIDRTDDTVAHLYDPLHPAVLHLVAHTIRMASRVK
                     MPVAVCGEMAGDPTVTRLLLGFGLREFSMHPAQLLAVKQQVLTTRIGDVEPLARRIGR
                     THDPEQARELLARLNGRAPV"
     gene            2126..3259
                     /locus_tag="VI319_14720"
     CDS             2126..3259
                     /locus_tag="VI319_14720"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY6865148.1"
                     /translation="MGHPGSVGAVAPRTMHFDEPLRFKSGAVLDAYDLVYETYGTLNP
                     ARSNAVLVCHALNAAHHVAGFYADDPQNVGWWDNMIGPGKPLDTERFFVVGVNNVGGC
                     FGSTGPKSVDPKTGKPYGSSFPVVTVEDWVNAQARLADRLGIDSFAAVMGGSLGAMQA
                     LQWTMSYPGRIRNSIVVAAAPRLSAQNIAFNEVARQAITTDPDFHGGDYYEHGVVPRR
                     GLRLARMIGHITYLSDDAMMEKFGRVLRAGAIGFHFDIDFEIESYLRHQGDKFSEYFD
                     ANTYLLITRALDYFDPAADHGHDLKKALALAKARFLVVSFTSDWRFPPERSREIVKGL
                     LDNRLDVSYAEIDAPHGHDAFLLEDPRYHALVRAYFDNVHAAL"
     gene            3256..>3361
                     /locus_tag="VI319_14725"
     CDS             3256..>3361
                     /locus_tag="VI319_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY6865149.1"
                     /translation="MTEHDQARLTLSGRSDFEAIAAWVRPDSSVLDLGC"
BASE COUNT          533 a         1179 c         1170 g          479 t
ORIGIN      
        1 cacccaggtc gcgagccgat gcggcgccga ggtctggctc tcgcgcgagg gccggcgggt
       61 gaacgcgaag agcatcatgg gcgtgatgat gctcgcggca ggcaagggta gccgggtgct
      121 ggtcgaggcc gaaggcgcgg acgcggacga cgcgatggcg gcgatcctgg cgctgatcgc
      181 ggccggcttc ggcgaagagg aatcgcgcga acgatgaacc gacgggacac gcagacgcaa
      241 tcggcgagct tcacgctgca cggcgcgcgc gtcggcgagg gcatcgcgat cggcttcgcg
      301 cacctgtttt cgaccgcgcg gctcgaggtc gcgcactacc aggtcaccga cgacgacgtc
      361 gaggccgagc tgtatcgcct ggaccgcgcc ttcgagcagg tgcgcggcga gctgcgcgcg
      421 ctgcaggacg gcctgccgcc cggcacgccg cccgagatgg gcgctttcct gcgcctgcac
      481 gcgatgatcc tcgacgacgc caatctcgcg gtcgcgccgc gcgagctggt gcgccggcac
      541 cgctacaacg ccgagcgcgc gctggtgcag cagatggacg agctggtcgc gcagttcgag
      601 cgcatggagg acgcgtacct gcgcgagcgc aagcaggacg tggtgcaggt cgtcgagcgg
      661 gtgctgaagg cgatgcaggg aacgggccac gcgccggccg cgcccgcgca cgaggagaac
      721 gtcatcgtcg tcgcgcacga cctctcgccc gccgacatga tgattttcaa gcagcatcag
      781 tacgcgggct tcgtcaccga cctggggggc gccacctcgc acacggcgat cgtcgcgcgc
      841 agcctcgcca ttcccgcgat cgtcggcctg cgccacgcgc gcgcgaccat ccgggagaac
      901 gacctgctga tcgtcgacgg cacgcacggc gtgctgatcg tcaatcccgg cgggctcgtg
      961 ctcgaggagt accggcgccg gcagggtgcg ctcgaggagg agcgccgcaa gctcaagggc
     1021 ctgcgcagca cgcgcgcgtc gacgctggac ggcgcgcgca tcgccctgca ggcgaacatc
     1081 gagctgccca aggacgtcgg ggaggcgcag gaggtcggcg cgggcggcat cggcctgttc
     1141 cgcacggagt tcctgttcct caaccgagac gacctgcctt ccgaggacga gcagttcgag
     1201 gcctaccgcg ccgtggcgca ggccatggcc ggccgcccgg tgacgatccg ctcgctcgac
     1261 ctgggcgccg acaagagcat caacggctcg cgcgcgggcc cgaacccggc gctcgggctg
     1321 cgcgcgatcc ggttctgcct cgccgagccg cagatcttcc tcgcgcagct gcgcgcgatc
     1381 ctgcgcgcgt cgcactacgg ccgggtgcgc ctgctcgtgc ccatgctcgc gcatgcgcac
     1441 gaggtcgagc aggcgctgca ggccgtgcgg caggcgaaag ccggcctgga cgaggagcgc
     1501 gtgccctacg acgccgcgct caaggtcggc gggatgatcg aggtgccggc ggcggcgctc
     1561 tcgctgaaat acctcatctc gaagctcgac ttcctctcga tcgggacgaa cgacctcgcc
     1621 cagtacacgc tcgcgatcga ccgcaccgac gacacggtgg cgcacctcta cgatccgctg
     1681 cacccggcgg tgctgcacct cgtcgcgcac acgatccgca tggcgagccg cgtgaagatg
     1741 ccggtcgcgg tgtgcggcga gatggccggc gacccgacgg tcacccggct gctgctcgga
     1801 ttcgggctgc gcgagttctc gatgcacccc gcccagctgc tcgcggtcaa gcagcaggtc
     1861 ctcacgacgc gcatcggcga cgtcgagccg ctcgcacgcc gcatcggccg cactcacgat
     1921 cccgagcagg cgcgcgagct gctcgcgcgc ctcaacggcc gggcgcccgt ttgacccgcg
     1981 ccggcgaatg cggtaacttg ccgcaagcgc cgatttgatc atcagcttgc gggcgcggag
     2041 gtggaaacac ctccaaatac ggctaaagcg agggcgaccc gtttagcgag caaagctgaa
     2101 cgggtttttt gtttttcggg cgcgaatggg acatccggga tccgtcgggg cggtggcgcc
     2161 ccggacgatg cacttcgacg agccgctgcg attcaaaagt ggcgcggtgc tcgacgccta
     2221 cgacctcgtg tacgagacgt acggaacgct gaacccggcg cgctcgaacg cggtgctcgt
     2281 gtgccacgcg ctgaacgccg cccaccacgt cgccggcttc tacgccgacg acccgcagaa
     2341 cgtcggctgg tgggacaaca tgatcgggcc cgggaagccg ctcgacaccg agcgcttctt
     2401 cgtggtcggg gtcaacaacg tgggcggctg cttcggctcg accgggccga agagcgtcga
     2461 cccgaagacc ggcaagccgt acggctcgag ctttcccgtg gtcacggtcg aagactgggt
     2521 gaacgcgcag gcgcgcctcg ccgaccgcct cggcatcgac tccttcgccg cggtgatggg
     2581 cggcagcctc ggggcgatgc aggcgctgca gtggacgatg agctaccccg ggcgcatccg
     2641 caactcgatc gtcgtcgcgg ccgcgcccag gctctcggcg cagaacatcg cgttcaacga
     2701 ggtggcgcgc caggcgatca cgaccgaccc ggacttccac ggcggcgact actacgagca
     2761 cggcgtcgta ccgcgccggg gcctgaggct cgcgcgcatg atcgggcaca tcacctacct
     2821 gtccgacgac gcgatgatgg agaagttcgg ccgcgtcctg cgcgcgggcg cgatcgggtt
     2881 ccacttcgac atcgacttcg agatcgagtc ctacctgcgc caccaaggcg acaagttctc
     2941 ggagtatttc gacgcgaaca cctatcttct gatcacgcgc gcgctcgact acttcgaccc
     3001 ggcggcggat cacggccacg acctgaagaa ggcgctcgcg ctcgcgaagg cgcgattcct
     3061 cgtggtctcg ttcacctcgg actggcgctt cccgcccgag cgctcgcgcg agatcgtgaa
     3121 gggcctgctc gacaaccgcc tcgacgtctc ctatgccgag atcgacgcgc cgcacggcca
     3181 cgacgcgttc ctgctcgagg acccgcgcta ccacgcgctg gtgcgcgcgt acttcgacaa
     3241 cgtccacgcg gcgctatgac cgagcacgac caggcgcgcc tcacgctgtc gggccgctcg
     3301 gacttcgagg cgatcgcggc ctgggtccgg ccggactcga gcgtgctcga cctgggctgc
     3361 g
//
LOCUS       DATDMW010000352         7170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2221417, whole genome shotgun sequence.
ACCESSION   DATDMW010000352 DATDMW010000000
VERSION     DATDMW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221417"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VI319_14730"
     CDS             <1..753
                     /locus_tag="VI319_14730"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY6865150.1"
                     /translation="EAPALENAGGGRTAPVPGDVEIAGGTLMLGAPRNSGYVFDTEKW
                     AHPVEVAPFAIARHPVTNAQFLEYVRAGGKAPRYWSERDGAWGERRFDRWAPLEPELP
                     VVHVDWHEAQAYCRWAGRRLPTEAEWEFAAGGGDKRRYPWGDATPSPDRANFHGARRA
                     PAGAFAPGDTPEGVRQMLGNVWEWTATTFDPYPGFVHDPYRDYSVPWFGTHKVLRGGS
                     FASPARLLRNTWRNFYTPDRYDVFAGFRTCRA"
     gene            741..1526
                     /locus_tag="VI319_14735"
     CDS             741..1526
                     /locus_tag="VI319_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY6865151.1"
                     /translation="MPGLKRIESRDNAQVKALVKLAASASERRRTGTTLIEGERLVGA
                     YRDSGGTAETIAVGESAYARPEIRSLVDAAPARVKLLLSDRVVEKVSQVVTASGVVAV
                     VKAPKPPEIPREVGAAVLIENLQDPGNLGSILRSAAAAGIREIFLSPGTVFAWSPKTV
                     RAGMGAHFFLTIHENVDLADIARRCKGRVIATEPRAKATLYETRLDGNVAWLFGNEGA
                     GVSEAARRIATDWVRIPMPGASESLNVAAAAAICLFEQVRQNQ"
     gene            1549..2604
                     /locus_tag="VI319_14740"
     CDS             1549..2604
                     /locus_tag="VI319_14740"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6865152.1"
                     /translation="MKKIVFALILALTALVAAAADYPAPKEADWIAREFRFHTGETLR
                     DVKLHYTTIGSPQNEPVLVLHGTGGNARTMLNAQFAGELFGAGQPLDAAKYFIILPDA
                     LGTGKSTKPSDGLRAKFPKYNYDDMVLAQYRLVTEGLGIRHLRLVIGNSMGGMQAWMW
                     GVQHPEFMDAIVPMACQPIQMSGRNWMMRRLLVESIRRNPDYNGGNYTAQPRGMQLAL
                     TEFAIATSGGTQAYFKQAPTREQADKLVFDRLDAAFRGDANDLLYQWESSDDYNPSPG
                     LERIQAVVLAINAADDERNPPEIGVMEPEMKRVKNGRLVIIPASAETRGHATTGMAKF
                     WKGYLAELLEKAPRRSQ"
     gene            complement(2598..3425)
                     /locus_tag="VI319_14745"
     CDS             complement(2598..3425)
                     /locus_tag="VI319_14745"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY6865153.1"
                     /translation="MSVPRRTVFFVSDGTGITVEMLGHSLLTQFEGIEFLHVTVPFVD
                     TVAKARACVARIEDAGRNGSARPVVFTTLVNGEVRDVIRAAGAFVLDFFEVFLDPLEG
                     ELGAKSSHTIGRSHSALDTKQYTNRIDAINFALAHDDGVSSRDLDKADVILVGVSRSG
                     KTPTSLYLALKFGIRAANYPLIPEDFDRLQLPTVLQDYRDKLFGLSISPERLTEIRHE
                     RRPNSRYANLDNCRFEVQQAERLMQREGVRWADSTTKSIEEIATTIMQDFKLERRVY"
     gene            3525..5930
                     /gene="ppsA"
                     /locus_tag="VI319_14750"
     CDS             3525..5930
                     /gene="ppsA"
                     /locus_tag="VI319_14750"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY6865154.1"
                     /translation="MSDQQYVVKLEQLRMGDVDKVGGKNASLGEMISQLSKAGIRVPG
                     GFATTAAAYRDFLAQGGLKDRIDARLKGLDADDTDALARCGAEIRGWIEQAPLPKRLE
                     QEVSAGYAELMKGGEASVAVRSSATAEDLHDASFAGQQETLLNVNGLPAVMKALKQVF
                     ASLYNDRAISYRIHKGFDHSAVALSAGIQRMARSDKGASGVMFTLDTESGFQDVVFIT
                     ASWGLGETVVQGAVNPDEFTVHKPMLRAGKYPIIRRGLGSKMHKMVFAEELKENEGKA
                     VETVDVPEAERHRFCLSDEDVIELARFALKIEEHYQRPMDIEWARDGIDGKIYILQAR
                     PETVKSRKTEGQERYRLKQRSEVLATGRAIGHKIGAGSVRVVKDSTQMARVKPGDILV
                     TDMTDPNWEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATQALKDGETCT
                     VSCAEGDTGHVYRGMLEIEVSSQQLGDMPKCPVKITMNIGNPQLAFDFQALPNWGVGL
                     ARLEFVINNMIGVHPKAVLEYAKLPSELKRQIDERARGYTDARSFYVEKLAEGVATIA
                     AAFWPKPVIVRLSDFKSNEYKKLLGGSRYEPDEETPILGFRGASRYIAKSFRDCFELE
                     CEAMRRVRNDMGLANVEIMVPFVRTLGECERVIGLLAEGGLKRGENGLRLIMMCEVPS
                     NAILAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGLVAEAFDERDPAVKAMLRLAIQAC
                     RKAGKYVGICGQGPSDNIDFARWLMQEGIESMSLNPDTVVDTWMQLAKASESPSRPKV
                     PAK"
     gene            complement(5950..6399)
                     /gene="smpB"
                     /locus_tag="VI319_14755"
                     /pseudo
     CDS             complement(5950..6399)
                     /gene="smpB"
                     /locus_tag="VI319_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
     gene            6435..6872
                     /locus_tag="VI319_14760"
     CDS             6435..6872
                     /locus_tag="VI319_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY6865155.1"
                     /translation="MVVVDRSVLAPYTANEMFALVDDVESYPQFLPWCESTRIALREP
                     GRTVATIRANYHGLHQEFTTENANRPGERIDITLVSGPFRTLRGHWGFTPLGERGCKI
                     EFRLEYQFASRILDKVAGPVFHKIADSFVDAFVRRAEQRLGRR"
     gene            6869..7162
                     /locus_tag="VI319_14765"
     CDS             6869..7162
                     /locus_tag="VI319_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEY6865156.1"
                     /translation="MSGATIRVEVAYASPERQALRTLTLPEGASAADALRASGLAEES
                     PEIDPATTELAVYGRPVRPEARLRDRDRVEIVRPLQADPKESRRSRAKRGAGR"
BASE COUNT         1189 a         2420 c         2491 g         1070 t
ORIGIN      
        1 gaggcgccgg cgctggagaa cgccggcggc gggcgcaccg ctcccgtgcc tggcgacgtg
       61 gagatcgccg gcggcacgct gatgctcggc gccccgcgca actcgggata cgtcttcgac
      121 accgaaaaat gggcgcaccc ggtcgaggtc gcgccgttcg cgatcgcgcg gcatcccgtg
      181 acgaacgccc agttcctcga gtacgtccgc gccggtggca aggcgccccg gtactggagc
      241 gagcgcgacg gcgcctgggg cgagcggcgc ttcgaccgct gggcgccgct cgagccggaa
      301 ctgccggtcg tgcacgtcga ctggcacgag gcgcaggcgt attgccgctg ggcagggcgg
      361 cggcttccga ccgaggcgga atgggaattc gcggcgggcg gaggggataa gcgccgatat
      421 ccctggggcg acgcgacgcc ttcgcccgac cgcgcgaact tccacggcgc gcgccgcgcg
      481 cccgccggcg cctttgcacc gggcgacacg cccgagggcg tccgccagat gctgggcaac
      541 gtctgggaat ggaccgcgac gacgttcgat ccgtatcccg ggttcgtcca cgatccttat
      601 cgcgactact ccgtgccctg gttcggcacg cacaaagtgc tgcgcggcgg cagcttcgcc
      661 agcccggcgc gcctgctgcg caacacctgg cgcaacttct acacgcccga ccgctacgac
      721 gtcttcgcgg ggttccgcac gtgccgggcc tgaagcgcat cgagtcgcgc gacaacgcgc
      781 aggtcaaggc gctggtgaag ctcgctgcgt ccgcatcgga gcggcgtcgc accggaacga
      841 cgctgatcga gggcgagcgc ctggtcggcg cgtaccgcga ctcgggcggc accgcggaga
      901 cgatcgcggt gggggagagc gcctacgcca ggcccgagat ccggtcgctc gtcgacgccg
      961 cgcccgcacg ggtcaagctg ctgctctccg accgcgtggt cgagaaggtg tcgcaggtgg
     1021 tcaccgcctc gggcgtcgtc gcggtggtca aggcgccgaa gccgcccgag atcccgcgag
     1081 aggtgggcgc ggcggtgctg atcgagaatc tccaggaccc gggcaacctc gggtcgattc
     1141 ttcgcagcgc ggccgccgcc ggcatccgcg agatctttct ctcgccgggg accgtcttcg
     1201 cgtggtcgcc gaagaccgtg cgcgcgggga tgggagcgca tttctttctc acgatccacg
     1261 agaacgtcga cctggccgac atcgcgcgcc ggtgcaaagg ccgggtgatc gcgaccgagc
     1321 cgcgggcgaa ggcgacgctc tacgagaccc ggctcgacgg caacgtcgcc tggctgttcg
     1381 ggaacgaagg cgcgggcgtg tcggaggccg cgcgccggat cgccaccgac tgggtgcgca
     1441 tccccatgcc gggcgcctcg gagtcactga acgtcgccgc cgccgcggcg atatgcctgt
     1501 tcgagcaggt caggcagaac caatgaattt acacgaagga ggatgccgat gaagaagatc
     1561 gtcttcgcgc tgatactcgc gttgacagcc ctcgtagccg ccgcggcgga ttatcccgcg
     1621 ccgaaggaag ccgactggat cgcgcgcgag ttccgcttcc acacgggcga gacgctccgc
     1681 gacgtgaagc tgcactacac cacgatcggc tcgccgcaaa acgagccggt gctggtgctg
     1741 catggcacgg gcggcaacgc ccgcacgatg ctcaacgcgc aattcgccgg cgagctgttc
     1801 ggtgccggac agccgctgga cgcggcgaag tacttcatca tcctgcccga cgcgctcggt
     1861 accgggaagt cgaccaagcc ctccgacggc ctgcgcgcga agttccccaa gtacaactac
     1921 gacgacatgg tgctcgcgca gtaccgcctg gtgaccgagg gactcgggat caggcacctg
     1981 cgcctcgtca tcggcaactc gatgggcggg atgcaggcgt ggatgtgggg cgtgcagcac
     2041 ccggaattca tggacgcgat cgtgccgatg gcttgccagc cgatccagat gtcgggccgc
     2101 aactggatga tgcggcggct gctggtcgag tcgatccgcc gcaacccgga ctacaacggc
     2161 ggcaactaca cggcgcagcc gcgcgggatg cagctcgcgc tgaccgagtt cgcgatcgcg
     2221 acctcgggcg gcacgcaggc ctacttcaag caggcgccga cgcgcgagca ggccgacaag
     2281 ctcgtcttcg atcggctcga cgcggcgttc cgcggcgacg cgaacgacct gctgtaccag
     2341 tgggaatcgt cggacgacta caacccgtcg ccggggctcg agcgcatcca ggccgtggtg
     2401 ctcgcgatca acgccgccga cgacgagcgc aatccgccgg agatcggcgt gatggagccg
     2461 gagatgaagc gcgtgaagaa cgggcgcctc gtgatcatcc cggcgagcgc cgagacgcgt
     2521 gggcacgcga cgacggggat ggcgaagttc tggaaggggt atctggccga gctgctggag
     2581 aaagcgccac gccggtctca gtagacccga cgctccagct tgaagtcctg catgatggtg
     2641 gtcgcgatct cctcgatcga cttggtcgtc gagtcggccc agcggacgcc ttcgcgctgc
     2701 atcagccgct cggcctgctg cacctcgaac cggcagttgt cgaggttcgc gtagcggctg
     2761 ttcgggcgcc gctcgtggcg gatctcggtg agccgctccg ggctgatcga aaggccgaac
     2821 agcttgtcgc ggtagtcctg gagaactgtc ggcagctgca ggcggtcgaa gtcttccggg
     2881 atgagcggat aattggccgc ccggatgccg aacttcagcg cgaggtacag gctggtcggg
     2941 gttttcccgc tgcgggacac gccgaccagg atgacgtcgg ccttgtcgag gtcgcgggac
     3001 gacacgccgt cgtcgtgggc gagggcgaag ttgatcgcgt cgatccggtt cgtgtactgc
     3061 ttggtgtcga gggcgctgtg cgagcggccg atggtgtggc tcgacttggc gccgagctcg
     3121 ccttccagcg gatcgaggaa cacctcgaag aagtcgagca cgaacgcccc tgccgcccgg
     3181 atcacgtcgc gcacttcgcc gttcacgaga gtcgtgaata ctaccggccg cgcgctgccg
     3241 ttgcggcccg catcctcgat gcgcgcgacg caggcgcggg ccttggcgac ggtgtcgacg
     3301 aagggcacgg tgacgtgcag gaactcgatg ccctcgaact gtgtgagcag gctgtggccg
     3361 agcatctcga ccgtgatgcc ggtgccgtcg gagacgaaga agacggtgcg tcgcggaacg
     3421 gacatggaag aagaggcgtt gaccccgggt cagcgccgag gcggcgctgc cgttaaaata
     3481 gcggtttcgc gccgaacgtt caacaaccga agaaggccgg tctcatgagc gatcaacaat
     3541 acgtcgtcaa gctggagcag ctgcggatgg gcgacgtcga caaggtcggg ggcaagaacg
     3601 cctcgctcgg cgagatgatc agccagctgt cgaaggccgg gatccgcgtc cccggcgggt
     3661 tcgccaccac cgcggcggcg taccgcgatt tcctggcgca gggcgggctc aaggaccgga
     3721 tcgacgcccg gctgaagggg ctcgatgccg acgacaccga tgccctggcg cgctgcggcg
     3781 ccgagatccg cggctggatc gagcaggcgc cgctgccgaa gcgcctcgag caggaagtct
     3841 ccgccggata cgccgagctg atgaaaggcg gcgaggcctc ggtcgcggtg cgctcgagcg
     3901 ccaccgccga ggatctccac gacgcctcgt tcgccgggca gcaggagaca ttactcaacg
     3961 tgaacggcct tcccgccgtg atgaaggcgc tgaagcaggt gttcgcgtcc ctctacaacg
     4021 accgcgccat ctcctaccgc atccacaagg gcttcgatca ttccgcggtc gcgctctcgg
     4081 ccggcatcca gcgcatggcg cgcagcgaca agggcgcgag cggcgtcatg ttcacgctcg
     4141 acaccgagtc cggcttccag gacgtggtct tcatcaccgc gtcgtggggc ctgggcgaga
     4201 cggtggtgca gggcgccgtc aaccccgacg agttcaccgt gcacaagccg atgctgcgcg
     4261 ccggcaagta cccgatcatc cgccgcggcc tcggctcgaa gatgcacaag atggtcttcg
     4321 ccgaagagct gaaggaaaat gagggcaagg ccgtcgagac cgtcgacgtg ccggaggccg
     4381 agcgccaccg cttctgcctg tccgacgaag acgtgatcga gctcgcgcgc ttcgcgctga
     4441 agatcgaaga gcactaccag cgcccgatgg acatcgaatg ggcccgggac gggatcgacg
     4501 ggaagatcta catcctccag gcgcgcccgg agaccgtgaa gtcgcgcaag accgaaggcc
     4561 aggagcgcta ccgcctgaag cagcgctccg aggtgctcgc gaccggccgc gccatcgggc
     4621 acaagatcgg cgcgggcagc gtgcgcgtgg tcaaggactc cacccagatg gcgcgcgtca
     4681 agcccggcga cattctcgtc accgacatga ccgaccccaa ctgggagccg gtcatgaagc
     4741 gcgccgccgc catcgtcacc aaccgcggcg gccgcacctg ccacgcggcg atcatcgcgc
     4801 gcgagctcgg catccccgcg gtcgtggggt gcggcgacgc gacccaggcg ctgaaagacg
     4861 gcgagacctg caccgtctcc tgcgccgagg gcgacaccgg tcacgtctac cgcggcatgc
     4921 tcgagatcga ggtgagctcg cagcagctcg gcgacatgcc gaagtgcccg gtgaagatca
     4981 cgatgaatat cggcaatccg cagctcgcgt tcgatttcca ggccctcccg aactggggcg
     5041 tgggcctcgc gcgcctcgag ttcgtcatca acaacatgat cggcgtgcac ccgaaggccg
     5101 tgctcgagta cgcgaagctg ccctcggagc tcaagcgcca gatcgacgag cgcgcgcgcg
     5161 gctacacgga cgcccgctcg ttctacgtcg agaagctcgc cgagggcgtg gccacgatcg
     5221 cggccgcgtt ctggccgaag ccggtcatcg tgcgcctctc cgacttcaag tcgaacgagt
     5281 acaagaagct cctcggcggc tcgcgctacg agcccgacga ggagaccccg attctcgggt
     5341 tccgcggcgc ctcgcgctac atcgcaaagt cgttccgcga ctgcttcgag ctcgagtgcg
     5401 aggcgatgcg ccgcgtgcgc aacgacatgg gcctcgccaa cgtcgagatc atggttccct
     5461 tcgtgcgtac gctcggcgag tgcgagcggg tcatcggcct gctcgccgaa ggcggactca
     5521 agcgcggcga gaacggtctg cgcctgatca tgatgtgcga ggtgccctcg aacgcaatcc
     5581 tggccgagca gttcctcgag cacttcgacg gcttctcgat cggctccaac gacctgacgc
     5641 agctcacgct gggactcgat cgcgactcgg gcctggtcgc cgaggcgttc gacgagcgcg
     5701 atcccgcggt gaaggccatg ctgcgcctcg ccatccaggc ctgccgcaag gccggcaagt
     5761 acgtcggtat ctgcgggcag ggaccctcgg acaacatcga cttcgcccgg tggctgatgc
     5821 aggaaggcat cgagagcatg tcgctcaacc cggatacggt cgtcgacacc tggatgcagc
     5881 tcgcgaaggc ctcggagtcg ccgtctcgcc cgaaggtccc ggcgaaatag ccaccgctcg
     5941 gaccgggtct caggtcttgg tccgcagcag gcgctggcgc tcgcgctgcc agtcgcgctc
     6001 cttctcggcc gcgcgcttgt cgtactgccg cttgcccttc gcgaggccga ccgcgagctt
     6061 cacccggccc cgggtgtagt gcaggtcgag cggcaccagc gtatagccgg cgcgctgcac
     6121 cttgccgatc agcttgcgga tctcttccga acgcaggagc agcttgcgcg tgcggaccgg
     6181 gtcggggtgc acgtgcgtgg acgccgtctc gagcgggctg acgtgcgctc cgatcaggaa
     6241 gacctcctcg ccctggacca ggacgtaggc ctccttgatc tggacgcgcc cggcccggat
     6301 cgccttgacc tcccagcctt cgagcgcgag gcccgcctcg tagcgctcct cgatgaagta
     6361 gtcgtggaag gccttgcggt tttcggcgat cgtcacggac gcggactttc cactagaatt
     6421 cggcgatttt agccatggtc gtcgtcgatc gctcggtgct cgcgccgtac accgcgaacg
     6481 agatgttcgc gctggtcgac gacgtcgagt cgtaccccca gttcctcccc tggtgcgaga
     6541 gcacccgcat cgcgctgcgc gagcccggcc gtaccgtcgc gacgatccgc gccaactacc
     6601 acgggctgca ccaggagttc accacggaga acgccaaccg gcccggcgag cgcatcgaca
     6661 ttacgctcgt ctcgggtccg ttccgcacgc tgcgcggcca ctggggcttc acgccgctcg
     6721 gcgagcgcgg ctgcaagatc gagttccgcc tcgagtacca gttcgcgagc cgcatcctgg
     6781 acaaggtcgc cggacccgtg ttccacaaga tcgccgacag cttcgtcgac gccttcgtgc
     6841 gccgcgcgga gcagcgcctg ggccggcggt gagcggcgcg accatccgcg tcgaggtggc
     6901 atatgcgtcg cccgagcggc aggcgctgcg cacgctgacg ctccccgagg gcgcgagcgc
     6961 cgccgacgcg ctgcgggcga gcggattggc ggaggaatct ccggagatcg atccggcgac
     7021 caccgagctc gccgtgtacg gcaggcccgt gcggcccgag gcgcggctgc gcgatcgcga
     7081 ccgggtcgag atcgtccggc cgctgcaggc ggatccgaag gagtcgaggc gctcgcgcgc
     7141 gaagcgcggc gccggacgct agcctagttg
//
LOCUS       DATDMW010000353         5998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_725456, whole genome shotgun sequence.
ACCESSION   DATDMW010000353 DATDMW010000000
VERSION     DATDMW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725456"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..585
                     /locus_tag="VI319_14770"
     CDS             142..585
                     /locus_tag="VI319_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HEY6865157.1"
                     /translation="MQLTRADIARMIPHAGRMCLLDGVVHWDENRIRCFSRSHRDADN
                     PMRSADRLSTLCGIEYAAQAMAAHGGLAGGTNGRPRAGYLASLRDVVCRRDRLDDLQQ
                     DLIIEAERLMGDEAHVVYRFTLRAGGLTLLSGRATVSLDAERPAP"
     gene            582..1301
                     /gene="fabG"
                     /locus_tag="VI319_14775"
     CDS             582..1301
                     /gene="fabG"
                     /locus_tag="VI319_14775"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY6865158.1"
                     /translation="MRRALVTGGSGALGAAISRRLGALGFHVLVHANTGPDRAERIAR
                     EIRDAGGSAGAVQFDVADRAASERALEKLLEDGPIQVLVNNAGVNDDAVMPGMRAEQW
                     DRVIDVSLNGFFNVTRPLLLPMMRTRWGRIVSMSSVSAILGNRGQANYAAAKAGLHGA
                     SKSLALEVASRGVTVNVVAPGIIESPMTRDGVPAGLVETLVPLKRAGAPDEVAALVAF
                     LCSDAAGYITGQTISINGGMA"
     gene            complement(1308..2195)
                     /locus_tag="VI319_14780"
     CDS             complement(1308..2195)
                     /locus_tag="VI319_14780"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY6865159.1"
                     /translation="MGVRVPSSAPRSSGQILSQGIRLALAASCLICAPARAQEKKPLW
                     ELGLGLAAIDFPHYRGSDQRKTWLLPFPFVVYRGDLLKADERGRMRGLFFRSENVELD
                     FSANGTVPVKSSENDARSGMPDLDATFELGPSLNFFLLRSRDQKTKLDLRLPVRGVVA
                     TDLHHANSAGWVFQPNVNVDVNDALGHPGWSFGVQAGVVYGNRRYHEYFYSVAPEFAT
                     PTRSAFDAPGGYGGATFITALSKRFPRFWAGGYVKVDYLRGAAFEDSPLVRRKENWTA
                     GFAIAWVLRESSTLVVDRE"
     gene            complement(2166..2252)
                     /locus_tag="VI319_14785"
     tRNA            complement(2166..2252)
                     /locus_tag="VI319_14785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2216..2218),aa:Leu,seq:cag)
     gene            2315..4567
                     /gene="rnr"
                     /locus_tag="VI319_14790"
     CDS             2315..4567
                     /gene="rnr"
                     /locus_tag="VI319_14790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY6865160.1"
                     /translation="MSTKKPARPARKDPHHAREAARYESPIASREFILDTLAHDGPLT
                     EDALAHRVGIASHEREAFERRVAAMEREGQLLRNRKGALLVAEKIHLIAGRVEGHPDG
                     YGFVVPDDGSPDLHLDPREMRKVLHRDRVMVREAGEDRRGRREAAIVEVIERANAKLV
                     GRLRQDRNFLYVEASDRRISQDVFVAPEDAGGAKPGEVVVVQLTAQPDAYAKPMGRVI
                     EVLGDATDPGIEIEIALRKHDLPFEFSERARAQAEQFPAAPTAEDGTGREDLRGLALV
                     TIDGESAKDFDDAVWSAPLGGAGAKGGRTGFRLIVAIADVSHYVRHGDALDLEARERG
                     NSVYFPRRVIPMLPERLSNGLCSLNPDVDRLCMACDMEISADGEIGRYRFYPAVMRSR
                     ARLTYTKVAAVIEDPKGAQARALGDLVPQIQLLYRLFQSLLRARARRGAIDFETVETE
                     FVFDDDGRLKRIDRVERNDAHRLIEECMLAANVCASDFLSSRKHPALYRVHEGPTPEK
                     LEALRQFLREFGLQLQGGEKPKAKDYAKLLDSVRNRPDVQLLQTVLLRSLQQAVYSPR
                     NVGHFGLAYEHYTHFTSPIRRYPDLLVHRSIKAALARQRYSPGDWEQLGQHCSQTERR
                     ADEATREVAAWLKTYYMKDRVGEEFDGSVSAVTGFGIFVALDDFYVEGLVHISELGQD
                     YFHFDPAKHQLLGDRTRTRYRLADRVRVRVVRVDLERSRIDFVLADDDEASGGRRTRF
                     LRTRSRDARS"
     gene            4564..5310
                     /gene="rlmB"
                     /locus_tag="VI319_14795"
     CDS             4564..5310
                     /gene="rlmB"
                     /locus_tag="VI319_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY6865161.1"
                     /translation="MKPTRALFGFHAVIARLRSDPSSVAEIYLDEDRKDGRAKDLASA
                     AQRAGLEVMRVPTKRLDGFYGGGRHQGVVAIVREAGAKEGLEDRLDALAEPPLLLVLD
                     GVTDPHNLGACLRVADAAGAHAVIAPKDRAAGLNATVSRVASGAAESVPYYMVTNLAR
                     TLDELKERNIWIVGADERAETTLYDADLPAGIAWVLGAEGEGMRRLTRERCDLLVRIP
                     MRGTVESLNVSVASGVCLFESARRRAGPRA"
     gene            complement(5315..>5998)
                     /locus_tag="VI319_14800"
     CDS             complement(5315..>5998)
                     /locus_tag="VI319_14800"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEY6865162.1"
                     /translation="FGVHTWIAVKPSNAPAFTVYEVVGWRLRWADSVVAIGGRRPDGP
                     WYGARAELLADLRGEPVDRLIARIDRLAQAYPYAGRYTAWPGPNSNTFVAWITRAVPE
                     LAVELPPTAIGKDYLPGAVWGTAPSGRGFQVSLFGFFALTASPVEGFEVNLCTMTFGF
                     NPFTSTLKLPVFGRIAVPMPSFTWRAPRRSVRLPAVQVATMANEASLHPWPTSAQAEE
                     IRETLRRAA"
BASE COUNT          916 a         2108 c         2142 g          832 t
ORIGIN      
        1 ggacgcggag ctcgagcgcg cgcgagtcac catcccggcc gcgcgctgcc tgccgctgct
       61 cgccgcgctc gcgcgcggcg ccgaggcgac ggtcgtgctc gactatctgg acggcggccg
      121 gctgcgcgtc gccgtgcaac catgcagctg acgcgcgccg acatcgcgcg catgatcccg
      181 cacgccgggc gcatgtgcct gctcgacggc gtggtgcact gggacgagaa ccgcatccgc
      241 tgcttcagcc ggtcgcaccg cgacgccgac aatcccatgc gctccgccga ccgcctctcc
      301 acgctatgcg gcatcgagta cgcggcgcag gccatggcgg cgcacggcgg tctcgccggg
      361 ggcacgaacg gccgcccgcg cgcgggctac ctcgcgagcc ttcgcgacgt ggtatgccgc
      421 agggaccggc tcgacgacct gcagcaggat ctcatcatcg aggccgagcg gctcatgggc
      481 gacgaggcgc acgtggtcta ccgcttcacg ctgcgcgcgg gcgggctgac gctcctcagc
      541 ggccgcgcga cggtgagcct cgacgcggag agaccggcgc cgtgaggcgc gcgctggtga
      601 ccggcggcag cggtgcgctg ggcgcggcga tcagccggcg gctgggcgcc ctcggctttc
      661 acgttctggt acacgcgaac accggcccgg atcgtgccga gcgcatcgcg cgcgagatcc
      721 gggacgctgg cggatccgcg ggagcggttc agttcgatgt cgccgaccgc gccgcttcgg
      781 agagggcgct cgagaagctg ctcgaggacg ggccgatcca ggtcctcgtc aacaacgcgg
      841 gcgtgaacga cgacgccgtc atgccgggca tgcgcgccga gcagtgggac cgcgtgatcg
      901 acgtttccct gaacggtttc ttcaacgtca cgcggccgct gctgctgccg atgatgcgca
      961 cgcgctgggg ccggatcgtc agcatgtcct cggtgtcggc gatcctcggc aaccgcggcc
     1021 aggccaacta cgcggccgcg aaggcgggcc tgcacggcgc atcgaaatcg ctcgcgctcg
     1081 aggtcgcgag ccgcggcgtc accgtgaacg tggtcgctcc gggaatcatc gagtcgccga
     1141 tgacgcggga cggcgtcccg gccggcctcg tggagaccct ggtgccgctc aagcgagccg
     1201 gcgcgcccga cgaggtcgct gcgctggtcg ccttcctgtg ctcggacgct gccggctata
     1261 tcacgggcca gacgatctcg atcaacggcg ggatggcctg agccgcctca ctcgcggtcg
     1321 accacgagcg tgctcgactc gcgcaggacc caggcgatgg cgaatcccgc ggtccagttc
     1381 tccttcctgc gcacgagcgg gctgtcctcg aaggcggcgc cgcggagata atccaccttc
     1441 acgtagccgc ccgcccagaa gcgcgggaag cgcttgctga gcgccgtgat gaacgtggct
     1501 ccgccgtatc cgcccggcgc gtcgaacgcg gagcgcgtcg gcgtcgcgaa ctccggggcg
     1561 accgaataga agtactcgtg gtagcggcgg ttgccgtaga ccacgcccgc ctgcacgccg
     1621 aagctccagc ccggatggcc gagcgcgtcg ttcacatcga cgttgacgtt cggctggaat
     1681 acccacccgg cgctgttcgc atggtgcagg tcggtggcga cgacgccgcg caccggcagg
     1741 cgcaggtcga gcttcgtctt ctggtcgcgc gagcgcagga ggaagaagtt gagcgacggg
     1801 ccgagctcga acgtcgcgtc gaggtcgggc atgccgctgc gcgcgtcgtt ctccgagctc
     1861 ttcaccggca cggttccgtt cgcgctgaag tcgagctcga cgttttcgct cctgaagaac
     1921 aggccgcgca tccggccgcg ctcgtcggcc ttcaggaggt cgccgcggta gacgacgaac
     1981 gggaagggca gcagccaggt cttgcgctgg tcggagccgc ggtagtgcgg aaagtcgatc
     2041 gccgcgagcc cgaggccgag ctcccagaga ggctttttct cctgtgcccg cgccggcgcg
     2101 caaatcaggc acgacgccgc gagcgcgagc cggatgcctt gggataatat ctgaccggaa
     2161 gagcgtggtg ccgaggaagg gactcgaacc cccacagtgt tgccaccgct aggacctgaa
     2221 cctagtgcgt ctaccaattc cgccacctcg gcgctggggc cgcgccgatt ccggtcttca
     2281 gcccgagcgc ggcattctat aggaacccaa ggaattgtca acgaaaaaac ccgcgcgacc
     2341 cgcgcgcaaa gacccgcatc acgcgcgtga agcggcgcgc tacgagtcgc cgatcgcgag
     2401 ccgcgagttc atcctcgaca cgctcgcgca cgacggcccg ctgaccgagg acgcgctcgc
     2461 gcaccgcgtc ggcattgcct cgcacgagcg cgaggcgttc gagcgccgcg tcgccgcgat
     2521 ggagcgcgag gggcagctgc tgcgcaaccg caagggcgcg ctgctcgtcg cggagaagat
     2581 tcacctgatc gcgggccgcg tcgaggggca tccggacggc tacggcttcg tcgtccccga
     2641 cgacggcagc cccgatctcc atctcgatcc gcgcgagatg cgcaaggtcc tgcaccgcga
     2701 ccgcgtcatg gtgcgcgagg cgggcgagga ccgccgcggg cgccgcgagg cggccatcgt
     2761 cgaggtgatc gagcgcgcca acgcgaagct cgtgggccgg ctgcggcagg atcggaactt
     2821 cctctacgtg gaagcctccg accgtcgcat cagccaggac gtattcgtcg cgcccgagga
     2881 cgcggggggc gcgaagcccg gcgaggtcgt ggtcgtgcag ctcaccgcgc agcccgacgc
     2941 gtacgcgaag ccgatgggac gcgtgatcga ggtcctcggg gacgcgaccg accccgggat
     3001 cgagatcgag atcgcgctgc gaaagcacga tctgcccttc gagttctccg agcgcgccag
     3061 ggcgcaagcc gagcaatttc ccgccgcgcc gacggcggag gacggcacgg gacgcgagga
     3121 cctgcgcggc ctcgcgctgg tcacgatcga cggcgagagc gcgaaggatt tcgacgacgc
     3181 ggtctggtcc gcgccgctcg gcggcgccgg cgcgaaaggc ggccggaccg gcttccggct
     3241 gatcgtcgcg atcgccgacg tgagccacta cgtgcggcac ggcgacgcgc tcgacctcga
     3301 ggcgcgcgag cgcgggaact cggtctattt cccgcgccgc gtgatcccca tgctgcccga
     3361 gcgcctttcg aacgggctgt gctcgctcaa cccggacgtc gaccggctgt gcatggcctg
     3421 cgacatggag atctccgcgg acggcgagat cggcaggtac cgcttctacc cggcggtgat
     3481 gcgctcgcgc gcacggctca cgtataccaa ggtcgcggcc gtgatcgagg atccgaaggg
     3541 cgcgcaggca cgggcgctcg gcgacctggt gccgcagatc cagctgctct accgcctgtt
     3601 ccagtcgctc ctgcgggcgc gcgcgcgccg gggagcgatc gacttcgaaa cggtcgagac
     3661 cgagttcgtt ttcgacgacg atgggcgcct gaagcgcatc gaccgcgtcg agcgcaacga
     3721 cgcgcaccgg ctgatcgagg agtgcatgct cgcggcgaac gtgtgcgcgt ccgacttcct
     3781 gtcgagccgg aagcaccccg cgctgtaccg cgtccacgag ggtcccaccc cggagaagct
     3841 cgaggcgctg cgtcagttcc tgcgcgaatt cgggctgcag ctgcagggcg gcgagaagcc
     3901 caaggcgaag gactacgcca agcttctcga cagcgttcgc aaccggccgg acgtgcagct
     3961 gctgcagacg gtgctgctgc gctcgctgca gcaggcggtc tacagcccga ggaacgtggg
     4021 ccacttcggg ctcgcctacg agcactacac gcacttcacc tcgccgatcc ggcgctatcc
     4081 cgacctgctc gtgcaccgct cgatcaaggc ggcgctcgcg cggcagcgct acagccccgg
     4141 ggactgggag cagctcggcc agcactgctc gcagaccgag cgccgggccg atgaggcgac
     4201 gcgcgaggtc gccgcgtggc tgaagaccta ctacatgaag gaccgcgtcg gcgaggagtt
     4261 cgacggctcg gtgagcgccg tcaccggttt cggcatcttc gtcgcgctcg acgatttcta
     4321 cgtcgagggc ctggtgcaca tctcggagct gggccaggac tacttccact tcgacccggc
     4381 gaagcaccag ctgctcggcg atcgaacccg cacgcgctac cggctcgccg accgcgtgcg
     4441 cgtgcgcgtg gtgcgcgtcg atctcgagcg cagccgcatc gacttcgtgc tcgccgacga
     4501 cgacgaggct tcgggcggcc ggcgcacgcg cttcctgcgc acgcgctcgc gcgacgctcg
     4561 ctcgtgaagc cgacgcgcgc gctgttcggc ttccacgcgg tgatcgcgcg gctgcgctcg
     4621 gacccttcat cggtggccga gatctatctc gacgaggacc gcaaggatgg gcgtgccaag
     4681 gacctcgcgt ccgcggcgca gcgcgcgggg ctcgaggtga tgcgcgtgcc gaccaagcgc
     4741 ctcgacggct tctacggcgg cggacggcac cagggggtgg tcgcgatcgt gcgcgaggcg
     4801 ggcgcgaagg agggcctcga ggaccgcctc gacgcgctcg ccgagccgcc gctcctgctc
     4861 gtcctcgacg gcgtcaccga tccgcacaac ctcggcgcct gcctgagggt cgccgatgcc
     4921 gccggcgcgc acgcggtgat cgcgccgaag gaccgcgcgg cgggcctcaa cgcgaccgtt
     4981 tcccgggtcg cgagcggcgc cgcggagtcg gtgccgtact acatggtcac caacctcgcg
     5041 cgcacgctcg acgagctgaa ggagcgcaac atctggatcg tcggcgccga cgagcgcgcc
     5101 gagacgacgc tctacgacgc cgaccttccg gcgggaatcg cgtgggtgct cggcgcggaa
     5161 ggagagggca tgcgccggct cactcgcgag cgctgcgacc tgctggtgcg catcccgatg
     5221 cgcggcacgg tcgagagcct gaacgtctcg gtggcgagcg gcgtgtgcct gttcgaatcg
     5281 gcccgccggc gcgccggccc gcgcgcgtag gaattcaggc cgcgcggcgc agcgtctcgc
     5341 ggatctcttc cgcctgcgcc gacgtcggcc agggatggag gctcgcctcg ttcgccatgg
     5401 tcgcgacctg caccgcgggc agacgcacgg agcgccgcgg cgcgcgccag gtgaacgacg
     5461 gcatcggcac ggcgatgcgc ccgaacaccg ggagcttgag cgtgctggtg aacgggttga
     5521 acccgaacgt catcgtgcac aggttgacct cgaagccctc caccgggctc gcggtcagcg
     5581 cgaaaaagcc gaacagcgac acctggaatc cgcgcccgct cggcgcggtc ccccagaccg
     5641 cgcccgggag gtaatccttg ccgatcgccg tcggcgggag ctcgaccgcc agctcgggca
     5701 ccgcccgcgt gatccaggcg acgaaggtgt tcgagttcgg accgggccac gcggtgtagc
     5761 ggcccgcgta cggataggcc tgggccaggc gatcgatgcg agcgatcagg cgatccaccg
     5821 gctcgccgcg caggtccgcg agaagctcgg cgcgcgcccc gtaccacggt ccgtcgggac
     5881 ggcggccgcc gatcgcgacg accgagtcgg cccagcgcag ccgccagccg accacttcgt
     5941 atacggtgaa cgccggagcg ttcgagggct tgaccgcgat ccaggtgtgc acgccgaa
//
LOCUS       DATDMW010000354         7932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1099492, whole genome shotgun sequence.
ACCESSION   DATDMW010000354 DATDMW010000000
VERSION     DATDMW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099492"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VI319_14805"
     CDS             <1..681
                     /locus_tag="VI319_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865163.1"
                     /translation="ESITALEQGVTLRQAAAGAAFIEYEATRSVSNRGMKAVRETKAR
                     LADASAAGLGLLAPRKDADWIRVGTLLALLVEPGPDWVVGTLCRISAESSATGEMLRF
                     GIRVLARRPKACWFHVPPEARAPDAPQEGWSERNFLAYFKRGILLDVELAPGVTGEML
                     VPHGAAKAGQVLDVPGEIATQHARVTALRESTPGYDRVAFEVLPPTVHKTSAGPESDA
                     PDPWKFAA"
     gene            complement(705..2372)
                     /gene="ettA"
                     /locus_tag="VI319_14810"
     CDS             complement(705..2372)
                     /gene="ettA"
                     /locus_tag="VI319_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY6865164.1"
                     /translation="MAQFVYSLRRVGRVVPPKREILKNISLNFFPGAKIGVLGLNGAG
                     KSTLLKIMALEDKDYEGEVLPMPNMKIGYLPQEPRLDPEKTVRDAVEEGLGDVLAAKK
                     RLEEVYAAYAEPDADFDKLAAEQARLEAIVNASHSDSADLQMEVAADALRLPPWESKI
                     GPLSGGEKRRVALCRLLLSKPDILLLDEPTNHLDAESVEWLEQYLQKFPGTVIAVTHD
                     RYFLDNAAGWILELDRGYGIPWEGNYSSWLEQKEQRLEQEAKAEAARIKTMQKELEWV
                     RQNPSGRRAKSKARLARFEEMSSYEYQKRNETSEIFIPVAERLGDSVIEFQNVSKAYG
                     ERLLIDDLSFKVPQGAIVGIIGPHGAGKSTLFRMITGKEKADKGKIAIGPTVKMAFVD
                     QSRDALEGDKSVWEYISGGADILTVGKFEMPSRAYVGRFNFKGGDQQKKVGTLSGGER
                     GRLHLAKTLMAGANVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLDRIA
                     THILAFEGESKVVFFAGNYHEYEEDKRKRLGEEGAKPKRLRYKPLAR"
     gene            2443..2688
                     /locus_tag="VI319_14815"
     CDS             2443..2688
                     /locus_tag="VI319_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865165.1"
                     /translation="MSRLLTVGLAIAALAGCSTAGREGEQYTATDTPAASATAQAQKN
                     CAGAGLAPREAQRFQVYSSDQGRMVDRLYYTCVKPER"
     gene            2688..3836
                     /locus_tag="VI319_14820"
     CDS             2688..3836
                     /locus_tag="VI319_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEY6865166.1"
                     /translation="MLRPLWAIGFRPFYLLAGLFAALSVPLWAARFAGWLGEWVYFSE
                     SVWHAHEMIFGYAFAVMVGFLFTAVRNWTGRPTPSGGTLAAIALAWLAARVLVFTPWT
                     ELAAIADAAFALAAAAGIAVPLIASGNRRNYFFIALLVLLGAVNLCFYLAKDDVLDAP
                     VGVGLEIALDLILFVIAVMGGRVIPMFTANAIPGAAPRRAPWLDRAALAAVLLLVVFD
                     AIGAPEEPMAVLAGLAALLHGARFLLWRPWRTVHRPLVWILHASYAWIVIHLALRAFG
                     ELGLIMPSLATHALTVGAIGGMTLGMMTRTARGHTGRPLEAGGGEVAAYVLIQLAALV
                     RVFGPMFFPALYLDAIIVSGALWSLAFLAFTVVFWPVLTRPRLDGRPG"
     gene            complement(3926..4726)
                     /locus_tag="VI319_14825"
     CDS             complement(3926..4726)
                     /locus_tag="VI319_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HEY6865167.1"
                     /translation="MNALLAKIGTRLARYLSEPRRQGTQIAVDPPAMLAAALRPGDVL
                     LVEGNTRISVAIKYLTQSTWSHATLYIGDALGPAAPGEEPKVLIEADVVDGVRAVPLS
                     AYAKMHTRICRPVGLSAGDLAKVIGYAVARIGHRYDMKNVLDLARFLLPTPPVPVRFR
                     RRLIAFGSGDPTRAICSTLIAQAFQSVGYPILPDVIQEKTGDPACMDCYRETLRIRHY
                     SLYTPRDFDISSYFEVVKPMLAAGFDYHKVAWAAQAPELQLAASSMSS"
     gene            complement(4746..5054)
                     /locus_tag="VI319_14830"
     CDS             complement(4746..5054)
                     /locus_tag="VI319_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865168.1"
                     /translation="MTMLPADAAACAKTPEHPPTALERVLRAMSVFTMLMTVPQVYTI
                     WVGHDARGVSIVSWGAYLIAALLWFVYGLQKGDKTIYLACVGWVVLDCAVVIGGIMYR
                     "
     gene            complement(5120..6478)
                     /locus_tag="VI319_14835"
     CDS             complement(5120..6478)
                     /locus_tag="VI319_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865169.1"
                     /translation="MKHAKVFLSSLALTLGGAAALTAAGLTLTALADDGPGRIANDND
                     RAIDRSAERMVEQGRGIFRFDTFGSEHFFGDALQLHQAIAGAANGGVGPGVSPKTALA
                     VGLKVDSDALPAALKAQIAAGQVNLDDPATTLALLKLNAVVGLTGKFDASGKITALGA
                     QCALCHSTVDNSFAPGIGKRLDGWSNQDLNIGLILSLAPNLKPFTDLLGVDAATVKAV
                     LTSWGPGKFDAELDKDGAAHAFRPDGKPAATLIPPAFGLAGVNLHTWTGFGSVPYWNA
                     YVAATEMHGSGTFFDQRLSDPAQFPVAAATGSWNTRGKPDLVTAKLPALHFYQLAIPA
                     PKAPAGSFDAAAAARGKVVFEGDGKCAVCHVPPLYTEPGHNLHSPEEMGIDSFQADRS
                     PTHAYRTAPLAGLWTHQKRGFYHDGRFPTLNDVVAHYDSHFGLGLGAQQKSDLVEYLK
                     SL"
     gene            complement(6509..7222)
                     /locus_tag="VI319_14840"
     CDS             complement(6509..7222)
                     /locus_tag="VI319_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6865170.1"
                     /translation="MQPATVSAVNARTDDAELVRRIAAGDHAACRELMRRYNQRLYRT
                     ARAILKDDAEAEDAVQEAYLLAFRAMGGFRGDAKLSTWLVRIAANEALGRLRKRSRAA
                     EIIPFDGIDGGDAVAEALEDDMMPSKPEQPENAALRAETRRLLEAKIDELPDAFRAVF
                     VLRALEELSVEETAATLGIPDATVRSRFFRARGMLRAALSRDIDVAFDQAFAFAGERC
                     DRIVANVMAKLEQAPPGSA"
     gene            7421..>7932
                     /locus_tag="VI319_14845"
     CDS             7421..>7932
                     /locus_tag="VI319_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY6865171.1"
                     /translation="MKRLALAAALLAASAAAAPAASNGPRAVATFESLGLYWTPPSDP
                     GAAGCAVQYRKAGDGAWKKGLDLWYDARNRECRGSLVRLSPGTAYEVRLAAPGATTTL
                     TARTWPERFPVARTVTVGGGSETLHITQGGSPDGYVLYTGAPGATIDVANKAANDVTI
                     AAPYVIVRGLV"
BASE COUNT         1091 a         2747 c         2798 g         1296 t
ORIGIN      
        1 gaatcgatca cggccctgga gcagggcgtg acgctgcggc aggcggcggc gggcgcggcc
       61 ttcatcgagt acgaagcgac gcgcagcgtg tccaaccgcg gcatgaaggc cgtgcgcgag
      121 acgaaggcgc gcctcgccga cgcgagcgcc gccggcctcg ggctgctggc cccgcgcaag
      181 gacgcggact ggatccgcgt gggaacgctg ctcgcgctcc tcgtcgagcc cgggccggac
      241 tgggtcgtcg gcacgctgtg ccgcatcagc gccgaatctt cggccacggg cgagatgctg
      301 cgcttcggga tccgcgtgct cgcgcgccgg cccaaggcct gctggttcca cgtgccgccc
      361 gaggcgcgcg cccccgacgc gccccaagag ggctggtcgg agcgcaattt cctggcgtat
      421 ttcaagcgcg ggatcctgct cgatgtcgag ctcgcgccgg gcgtcacggg cgagatgctg
      481 gtgccgcacg gcgcggcgaa ggcgggccag gtcctcgacg ttcccggcga gatcgccacg
      541 cagcacgccc gcgtcaccgc gctgcgcgag tcgaccccgg gctacgaccg ggtggcgttc
      601 gaggtgctgc cgccgaccgt gcacaagacg agcgccgggc ccgagtcgga cgcgcccgat
      661 ccctggaaat tcgccgcgtg attcgaaact gagtcgaaac cgggctatcg cgcgagcggc
      721 ttgtagcgca ggcgcttcgg cttcgcacct tcctcgccga ggcgcttgcg cttgtcttcc
      781 tcgtactcgt ggtagttgcc ggcgaagaac acgaccttcg attcgccctc gaaggcgagg
      841 atgtgggtcg cgatgcggtc gaggaaccag cggtcgtgcg agatcaccat cacgctgccc
      901 gcgaactcga gcagcgcgtc ctcgagcgcg cgcagcgtct cgacgtcgag atcgttcgac
      961 ggctcgtcga gcagcagcac gttcgctccc gccatcagcg tcttcgcgag atgcagccgg
     1021 ccgcgctcgc cgcccgacag cgtgccgacc ttcttctgct ggtcgccgcc cttgaagttg
     1081 aagcgcccga cgtaggcgcg cgagggcatc tcgaacttgc cgacggtgag gatgtcggcg
     1141 ccgcccgaga tgtattccca cacgctcttg tcgccctcga gcgcgtcgcg cgactggtcg
     1201 acgaaggcca tcttgacggt ggggccgatc gcgatcttgc ccttgtcggc cttctccttc
     1261 cccgtgatca tgcggaacag cgtcgacttg ccggccccgt gcggtccgat gatgccgacg
     1321 atcgcgccct gcgggacctt gaacgacagg tcgtcgatca gcagccgctc gccgtaggcc
     1381 ttgctcacgt tctggaactc gataaccgag tcgccgaggc gctcggcgac cgggatgaag
     1441 atctccgagg tctcgttgcg cttctggtac tcgtaggacg acatctcctc gaagcgcgcg
     1501 aggcgcgcct tgctttttgc ccttcgtccg gaaggatttt gccggaccca ctccagctct
     1561 ttctgcatgg tcttgatgcg cgcggcctcg gccttcgcct cctgctcgag gcgctgctcc
     1621 ttctgctcga gccacgacga gtagttgcct tcccagggaa tgccgtagcc gcggtcgagc
     1681 tcgaggatcc agccggcggc gttgtcgagg aagtagcggt cgtgcgtcac cgcgatcacc
     1741 gtgccgggga acttctggag atactgctcg agccactcga cgctctcggc gtcgaggtgg
     1801 ttggtcggct cgtcgagcag caggatgtcg ggcttggaga gcagcagccg gcacagcgcg
     1861 acgcggcgct tctcgccgcc cgagagcggg ccgatcttcg actcccacgg cggcaggcgc
     1921 agcgcgtcgg cggcgacctc catctgcagg tcggcgctgt ccgagtgcga ggcgttcacg
     1981 atcgcctcga gccgcgcctg ctcggcggcg agcttgtcga agtcggcgtc gggctcggcg
     2041 taagccgcgt agacttcctc gagccgcttc ttcgcggcga gcacgtcgcc caggccctcc
     2101 tcgaccgcgt cgcgcacggt cttctcggga tcgagccggg gctcctgcgg caggtagccg
     2161 atcttcatgt tcggcatcgg caggacctcg ccctcgtagt ccttgtcctc gagcgccatg
     2221 atcttcagca gcgtggactt gcccgcgccg ttcaggccga gcacgccgat cttggcgccg
     2281 ggaaagaagt tgagcgagat gttcttcagg atctcgcgct tcggcgggac gaccctgccc
     2341 accctgcgga gggaatagac gaattgagcc atgatttacc gcggattgac ggaagacccg
     2401 aagcttaacg cgtacaatac gcggctccaa gagggagaag acatgagccg gctcctgacg
     2461 gtgggattgg cgatcgcggc gctcgcgggc tgctcgaccg ccgggcgcga gggcgagcaa
     2521 tacaccgcga ccgacacccc ggccgcgagc gcgacggccc aggctcagaa gaactgcgcc
     2581 ggcgcggggc tggcgccgcg cgaggcccag cgcttccagg tgtacagctc cgaccagggc
     2641 cgcatggtcg accgcctgta ttacacctgc gtcaagccgg agcggtagtg ctcagaccgc
     2701 tctgggccat agggttccgc cccttctatc tcctcgccgg cctcttcgcc gcgctctcgg
     2761 tcccgctctg ggccgcgcgc ttcgccggct ggctcggcga atgggtgtat ttctcggaat
     2821 cggtctggca cgcgcacgag atgatcttcg gctacgcctt cgccgtgatg gtcggcttcc
     2881 tgttcaccgc ggtgcgcaac tggaccgggc gcccgacgcc gagcggcggc acgctcgccg
     2941 cgatcgcgct cgcgtggctc gcggcgcgcg tgctcgtgtt cacgccgtgg accgagctcg
     3001 ccgccatcgc cgacgccgcc ttcgcgctcg ccgccgcggc cggcatcgcg gtgccgctga
     3061 tcgcgagcgg caaccgccgc aattatttct tcatcgcgct gctggtcctc ctcggcgcgg
     3121 tcaacctgtg cttctatctc gccaaggacg acgtcctcga cgcgccggtc ggcgtcgggc
     3181 tcgagatcgc gctcgacctg atcctgttcg tcatcgcggt gatgggcggg cgcgtgattc
     3241 ccatgttcac ggcgaacgcg atcccgggcg ccgctccgcg gcgcgcgccg tggctcgatc
     3301 gcgccgcgct cgccgccgtg ctgctgctcg tcgtgttcga cgcgatcggc gcgcccgagg
     3361 agccgatggc cgtgctcgcc ggcctcgccg cgctgctgca cggcgcgcgc tttctcctgt
     3421 ggcggccctg gcgcacggtc catcgcccgc tcgtgtggat cctgcacgcg tcgtacgcgt
     3481 ggatcgtcat ccacctcgcg ctgcgcgcct tcggcgagct ggggctgatc atgccgagcc
     3541 tcgccacgca cgcgctcacc gtcggcgcca tcggcggcat gacgctcggc atgatgacgc
     3601 gcaccgcgcg cggccacacc ggccggccgc tcgaggcggg cggcggcgag gtcgcggcgt
     3661 acgtcctgat ccagctcgcc gcgctggtgc gcgtgttcgg cccgatgttc ttccccgcgc
     3721 tctatctcga cgcgatcatc gtctcggggg cgctgtggtc gctcgccttc ctcgcgttca
     3781 cggtcgtctt ctggccggtg ctgacgcgcc cgcgcctcga cggccgcccg ggctgaatgc
     3841 ccgagcttcc cgacgtcgcc ggaatgtcat tctgagcgca gcgaagaatc tgctttacgg
     3901 cgcaaaagca gatccttcgc tgcgctcagg atgacatcga ggaggccgcg agctgcagct
     3961 ccggcgcctg cgccgcccag gcgaccttgt gatagtcgaa gccggcggcg agcatgggct
     4021 tgacgacctc gaagtacgac gagatgtcga agtcgcgcgg cgtgtacagg ctgtagtggc
     4081 ggatgcgcag cgtctcgcgg tagcagtcca tgcacgccgg gtcgccggtc ttctcctgga
     4141 tcacgtcggg caggatcggg tagccgaccg actggaacgc ctgcgcgatc agcgtcgagc
     4201 agatcgcgcg cgtcgggtcg cccgagccga acgcgatcag gcgccgcctg aagcgcaccg
     4261 gcaccggcgg cgtcggcagg aggaagcgcg cgaggtcgag aacgttcttc atgtcgtagc
     4321 gatggccgat gcgcgcgacc gcgtagccga tcaccttcgc gagatcgccg gccgagaggc
     4381 cgaccgggcg gcagatgcgc gtgtgcatct tcgcgtacgc ggacagcggc accgcgcgca
     4441 cgccgtcgac gacgtcggcc tcgatcagga ccttcggctc ctcgcccggt gctgcgggcc
     4501 cgagcgcgtc gccgatgtac agcgtcgcgt gcgaccaggt cgactgcgtc aggtacttga
     4561 tcgcgacgct gatccgggtg ttgccctcca cgagcagcac gtcgccgggc cggagcgccg
     4621 cggcgagcat cgccggcggg tcgacggcga tctgcgtgcc ctgccgcctc ggctcggaga
     4681 ggtagcgcgc gagccgggtt ccgatctttg cgagcagcgc gttcattcga ttctcccttc
     4741 gcgccttacc ggtacatgat accgccgatc accaccgcgc aatcgagcac gacccagccg
     4801 acgcacgcga ggtagatcgt cttgtcgccc ttctgcagcc catagacgaa ccagagcagc
     4861 gccgcgatca ggtacgcgcc ccaggacacg atcgacacgc cgcgcgcgtc gtgcccgacc
     4921 cagatcgtat acacctgggg cacggtcatc agcatcgtga acacggacat cgcgcgcagc
     4981 acgcgctcga gcgcggtcgg gggatgctcg ggcgtcttcg cgcacgcggc ggcgtcggcg
     5041 ggaagcatgg tcatggtttt ctcctcccca aagaaaacgc cgcggccggt tcctgcgccg
     5101 cggcgttcat tgtgactcct tacagcgact tcaggtactc gaccaggtcg cttttctgct
     5161 gcgcgccgag ccccaggccg aagtggctgt cgtagtgcgc caccacgtcg ttcagcgtcg
     5221 ggaagcggcc gtcgtggtag aagcctcgct tctggtgcgt ccacaggccg gcgagcggcg
     5281 cggtgcggta cgcgtgcgtc ggcgagcggt cggcctggaa cgagtcgatc cccatctcct
     5341 cgggcgagtg caggttgtgg ccgggctcgg tgtagagcgg cggcacgtgg cagaccgcgc
     5401 acttgccgtc gccttcgaac accaccttgc cgcgagcggc cgcggccgcg tcgaagctgc
     5461 ccgccggcgc cttcggagcg ggaatcgcga gctggtagaa gtgcagcgcc ggcagcttcg
     5521 ccgtgacgag gtcgggcttg ccgcgcgtgt tccacgagcc cgtggccgcc gcgaccggga
     5581 actgcgccgg gtcggagagc ctctggtcga agaacgtgcc cgagccgtgc atctcggtcg
     5641 cggcgacgta cgcgttccag tacggcaccg agccgaagcc cgtccaggtg tgcaggttca
     5701 cgccggcgag gccgaacgcg ggcgggatca gcgtggccgc gggcttgccg tcggggcgga
     5761 acgcgtgcgc cgcgccgtcc ttgtcgagct ccgcgtcgaa cttgccgggg ccccagctcg
     5821 tgagcaccgc cttcacggtc gccgcgtcga cgccgagcag gtcggtgaag ggcttcaggt
     5881 tgggcgcgag cgacaggatg aggccgatgt tgaggtcctg gttggaccag ccgtcgagcc
     5941 gcttgccgat gccgggcgcg aacgagttgt cgacggtcga gtggcacagg gcgcactgcg
     6001 cgccgagcgc ggtgatcttg ccgctcgcgt cgaacttgcc ggtgaggccg acgaccgcgt
     6061 tcagcttgag cagcgcgagc gtcgtcgccg ggtcgtcgag gttcacctgg ccggccgcga
     6121 tctgcgcttt cagcgccgcg ggcagcgcgt cggagtcgac cttcaggccg accgcgagcg
     6181 cggtcttggg cgagacgccg ggcccgacgc cgccgttcgc ggcgcccgcg atcgcctggt
     6241 gcagctgcag cgcgtcgccg aagaaatgct cggagccgaa cgtgtcgaag cggaagatgc
     6301 cgcggccctg ctcgaccatg cgctcggcgc tgcggtcgat ggcgcggtcg ttgtcgttgg
     6361 cgatgcgccc gggaccgtcg tcggcgagcg cggtgagggt gagaccggcc gcggtcagcg
     6421 cggccgcgcc gcccagcgtc agggcgagcg aagacagaaa cacctttgcg tgcttcattg
     6481 gaacctcctg ctgtggggtg aaacgggttt aggcgctacc aggcggcgct tgctcgagct
     6541 tggccatgac gttggccacg atgcggtcgc agcgctcgcc cgcgaaggcg aacgcctggt
     6601 cgaaagcgac atcgatgtcg cgcgacagcg cggcgcgcag catgccccgg gcgcggaaga
     6661 agcgcgagcg cacggtcgcg tcgggaatgc cgagcgtggc cgcggtctcc tcgaccgaca
     6721 gctcctcgag cgcgcgcagc acgaacaccg cgcggaaggc gtcgggcagc tcgtcgatct
     6781 tcgcctcgag caggcggcgg gtctccgcgc gcagcgcggc gttctcgggc tgttcgggct
     6841 tggatggcat catgtcgtct tccagggctt cggcgacggc gtcgccgccg tcgataccgt
     6901 cgaatgggat gatctcggcg gcccgggagc gcttgcgcag gcgcccgagc gcctcgttgg
     6961 cggcgatgcg caccagccag gtcgagagct tcgcgtcgcc gcggaagccg cccatcgcgc
     7021 ggaacgcgag caggtaggct tcctggaccg cgtcctcggc ctcggcgtcg tccttcagga
     7081 tggcgcgcgc ggtgcggtac agccgctggt tgtagcgacg catgagctcg cggcacgcgg
     7141 cgtgatcccc ggcggcgatc cggcgcacga gctcggcatc gtcggtacgg gcgttcactg
     7201 cgctgaccgt ggcgggctgc atgttcgtct ccttcgttcg cgccgattgg atgatgcgcg
     7261 caggccgcgc gttcccgcgc cttcgatccg cagtaacccc ttgccacgca tgggattgcg
     7321 cggccggtag tccacgcgga ccatggatgc ttccatatac cacgtctgcg gcccgttcag
     7381 actgtgcgcg atttcacctg ggcggaggcc cgaggctgcc gtgaagcgac tcgcactggc
     7441 cgccgcgctg ctcgccgcgt ccgccgcggc cgcgccggcg gcgtcgaacg gtccgcgcgc
     7501 cgtggcgacg ttcgagtcgc tggggctgta ctggacgccc ccctcggatc cgggcgccgc
     7561 gggctgcgcg gtgcaatacc gcaaggccgg ggacggcgcc tggaagaaag gcctcgatct
     7621 ctggtacgac gcgcgcaacc gcgaatgccg cggcagcctg gtgcgtcttt cgcccggcac
     7681 ggcctacgag gtgcggctcg cggcgccggg cgccacgacg acgctcaccg cgaggacctg
     7741 gccggagcgc tttccggtcg cgaggacggt cacggtaggc ggcggctccg agacgctgca
     7801 catcacgcag ggcggaagcc ccgacggcta cgtgctctac accggcgcgc ccggggcgac
     7861 gatcgacgtc gccaataagg ccgccaacga cgtgaccatc gcggcgcctt acgtgatcgt
     7921 gcgcgggctc gt
//
LOCUS       DATDMW010000355         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1597499, whole genome shotgun sequence.
ACCESSION   DATDMW010000355 DATDMW010000000
VERSION     DATDMW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597499"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VI319_14850"
     CDS             complement(<1..238)
                     /locus_tag="VI319_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865172.1"
                     /translation="MKSRMAVLALGFGLAFAAPLSAAPADPGRIVTLVCTQFTSNICT
                     SLEQVAADGVLTPYTAPPSGFVLVVTDFVWKAAHV"
     gene            complement(379..>2107)
                     /locus_tag="VI319_14855"
     CDS             complement(379..>2107)
                     /locus_tag="VI319_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEY6865173.1"
                     /translation="AKIPAYEMIRFSVTIQRGCFGGCTFCSITEHEGRIIQNRTEGSI
                     LLDARADLLVYGNAERAIVEIAHRLAKRERISTITDLRGTAFVRRAPPEGWIEIDSTE
                     VDTPGPLAPPVDPYAMEPARQQAQAPSNVRVVKFERQVPNAMRAKSVVRMPAFEQVRD
                     DPVLYAHASRILHVESNPGNARALVQRHGDADVWLNPPPVPLATKEMDALYELPYKRV
                     PHPAYGSAKIPAYEMIRFSIAIQRGCFGGCTFCSITEHEGRIIQSRSEESVLREIETV
                     RDTVPGFTGVISDLGGPTANMYRLACKSREIESACRRPSCVFPDICPNLNTDHAPLVK
                     LYRKAREIPGVKKVLIASGVRYDLAVKSPEYVRELAQHHVGGYLKIAPEAISEGPLAM
                     MMKPGVGAYDKFKALFDRFSKEAGKEQYLIPYFIAAHPGTTDADMLELALWLKSNGFR
                     ADQVQAFLPGPMATATAMYHTGRNPLRKITRDGERVTVPKGLRVRRLHKAFLRYHDPE
                     NWPVLRDALKRMGRADLIGPGRRCLVPAWQPEGTGLRHEGRRTTPFRTQHTGLPDARR
                     ARKLGKTGR"
BASE COUNT          308 a          727 c          734 g          338 t
ORIGIN      
        1 cgacgtgcgc cgccttccac acgaagtccg tcacgaccag cacgaagccg gacggcgggg
       61 ccgtatacgg cgtgagcacg ccgtcggcgg ccacctgctc gaggctggtg cagatgttcg
      121 acgtgaactg cgtgcagacg agcgtcacga tccgccccgg atcggccggc gcggccgata
      181 gcggagcggc gaaagcgaga ccgaagccca atgcaagcac tgccattctg cttttcatgc
      241 gtgctctcct ttggctgcga aggaacgaag cgattcgtca gcgcccgacg atacccgtcc
      301 gcggggccgc aagcccggac tttgcgacga accggctgaa gaggcgacgg agcgcgggaa
      361 aaggcccccg aagaggcttc agcgtccggt ctttccgagt ttgcgcgctc tgcgcgcgtc
      421 cggcaacccg gtgtgctggg tgcggaaggg cgtcgtccgg cgcccttcgt gccgcaggcc
      481 cgtcccctcg ggctgccacg ccggcacgag acagcgcctc cccggcccga tcaggtccgc
      541 gcgccccatg cgcttcagcg cgtcgcgcag caccggccag ttctccggat cgtggtagcg
      601 caggaacgcc ttgtgcagcc gccgcacgcg caggcccttc ggcaccgtca cgcgctcgcc
      661 gtcgcgcgtg atcttgcgca aggggttcct cccggtgtgg tacatcgcgg tcgcggtcgc
      721 catcgggccc ggcaggaacg cctgcacctg gtcggcgcgg aagccgttgc tcttcagcca
      781 cagcgcgagc tcgagcatgt cggcgtccgt cgtccccggg tgcgcggcga tgaagtaggg
      841 gatgaggtac tgctccttgc ccgcctcctt cgagaacctg tcgaagagcg ccttgaactt
      901 gtcgtaggcg ccgacgccgg gcttcatcat catcgcgagc ggcccctcgg agatcgcctc
      961 gggcgcgatc ttgagatagc cgccgacgtg gtgctgcgcg agctcgcgca cgtactcggg
     1021 cgacttcacc gcgaggtcgt agcgcacgcc cgaggcgatc agcaccttct tcacgccggg
     1081 gatctcgcgc gctttgcgat agagcttcac gagcggcgcg tggtcggtgt tgaggttggg
     1141 gcagatatcc gggaacacgc acgacgggcg ccggcacgcc gactcgatct cgcggctctt
     1201 gcacgcgagc cggtacatgt tcgccgtcgg cccgcccagg tcggagatga cgcccgtgaa
     1261 cccgggcacg gtgtcgcgca ccgtctcgat ctcgcgcagc accgactcct ccgaccggct
     1321 ctggatgatg cgcccctcgt gctcggtgat cgagcagaag gtgcagccgc cgaagcagcc
     1381 gcgctggatc gcgatcgaga agcggatcat ctcgtacgcc gggatcttcg cgcttccgta
     1441 cgccggatgc ggcacgcgct tgtacggcag ctcgtagagc gcgtccatct ccttcgtcgc
     1501 gagcggcacg ggcggcgggt tcagccacac gtcggcgtcg ccgtggcgct gcaccagcgc
     1561 gcgcgcgttg cccgggttcg actcgacgtg caggatgcgc gaggcgtggg cgtagagcac
     1621 cgggtcgtcg cgcacctgct cgaacgcggg catgcgcacg acgctcttcg cgcgcatcgc
     1681 gttcgggacc tggcgctcga acttgacgac gcgcacgttc gaaggcgcct gcgcctgctg
     1741 ccgcgccggc tccatcgcgt acggatcgac gggcggcgcg agcggccccg gcgtgtcgac
     1801 ttcggtcgaa tcgatctcga tccagccctc gggcggcgcg cggcgcacga acgcggtgcc
     1861 gcgcaggtcg gtgatcgtgg agatgcgctc gcgcttcgcg agccggtgcg cgatctcgac
     1921 gatcgcgcgc tcggcgttgc cgtagacgag caggtccgcg cgcgcgtcga gcaggatcga
     1981 gccctcggtg cgattctgga tgatccttcc ctcgtgctcg gtgatcgagc agaacgtgca
     2041 accgccgaag cagccgcgct ggatcgtgac ggagaagcgg atcatctcgt acgccgggat
     2101 cttcgcg
//
LOCUS       DATDMW010000356         8718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_851103, whole genome shotgun sequence.
ACCESSION   DATDMW010000356 DATDMW010000000
VERSION     DATDMW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851103"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VI319_14860"
     CDS             <1..910
                     /locus_tag="VI319_14860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865174.1"
                     /translation="CSAQGYPAKPVLVVMPLQAGSAVDVMIRIVAQRMSENLGQQILI
                     ENQPGAAGSIGAERVKRAAPDGYTVGVLNDSILTMIPNIRHVGYDPTKDFEPVGVVAA
                     ITWVMVVNNDVPAKTVREFIDYAKARPGKIDYSSGGNGSPQHVAMEAFKAATGLDLVH
                     IPYKGATQAAIDIMSGRVQAHFGAVSIVQPYVKDGRMRALGVPSAARSPLLPEVPTMA
                     EAGVPGFEWRTWASFVVPLRTPKPVVDRLNAELVRAIKAPDVRDKLIAQGLEPVGSGP
                     ETVTRWTRDGLARMRDIVKRANIQAE"
     gene            907..1566
                     /locus_tag="VI319_14865"
     CDS             907..1566
                     /locus_tag="VI319_14865"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6865175.1"
                     /translation="MSRVLTRIDHVTIGARNLDEGIDAYRRIGFDVDDHGAAFAEDDG
                     LVLVGGGEGLRSIALRSDDGTFARAPAQWIDIVPHRPVPARRHPNRVQRIERVYIAVT
                     DLAAAVEKVRSALGFAEPKMERGTVIDADMAIFQLGTTGLGLAQPAGPGVCADALRSR
                     GPGPFQVLFRTGSMDAAAKWIADHGVPPPKRGVRNTGEQAMLVTPEHACGVYIGFVGP
                     A"
     gene            1755..3848
                     /locus_tag="VI319_14870"
     CDS             1755..3848
                     /locus_tag="VI319_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6865176.1"
                     /translation="MGTEHIKVLVVEDVEMDAELALRELKRAGLRCVARRVDTEDAFR
                     RELGEFRPHVIISDFSMPRFDGMEAFAIAREVDPDVPFIFVSGTIGEEYAVRALKNGA
                     TDYVLKTNLIRLPPAVERAVRDAEARVEARRIEDARRKIERRFQALIENSADAIVLID
                     PEGAVLYRSPATGRLLGYTDEERAGRPVIELTHPEDAAALKAKLGESLNAPGKPIAFQ
                     CRARHKTDGWKILDGSFNNLTADDAIAAVVINCRDITASKEAEQRLAYLAQFDPLTGL
                     PNRDLFKDRLEQTLAQARRNDWLVGVVLVNLDRFKMVNDTLGVGAGDALLTQAAQRLG
                     DTLRGGDTAARLSGDEFAIALSNLARADDAATAAQKVLDALGRPFEVGGRQVYVSASV
                     GVALYPEDGAAGDVLLKNANAAKDRVKEQGGSGYQFYLPEMNERAAQRLQLETALRGA
                     LERREFELHYQPKASLATGAISGFEALLRWTQSDRRLASPARFVPILEDTGLIMPVGD
                     WVLATACAQIRAWQHRGLGSRSVAVNLSARQFQRPGLDATIARLIEETGIDPRQLELE
                     LTETLLMRDAEQAIRVLRTLKSYGVRISVDDFGTGYSSLAYLKRFPLDALKIDRAFVH
                     DVNNDADDAAITIAIINLAHSLKLKVIAEGVETQAQLHFLREHGCDEIQGYFFERPQP
                     AGDCTRLLQGRRTLG"
     gene            4035..7031
                     /locus_tag="VI319_14875"
     CDS             4035..7031
                     /locus_tag="VI319_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6865177.1"
                     /translation="MLAILAGFAALFAVVWQAPPHHALEGISSYLPLHNLLEGASIVV
                     AMLVFGVTWNAYTRERSGNTVILACGLLAVGLIDFAHMLSFKGMPDFVTPADPEKAIE
                     FWLAGRTVFAACLLSAAVRAPAPLASPATRYALLLGALAVTALVYWMVLLHGDRLPHT
                     FIEGKGLTPLKRAIEYAIVVLLAIPALLFYAKARLTASGDAALLFAAAAISILGELSL
                     TLYSAVTDVFNLLGHVYKIAAFAFIYRAVFVASVRQPFERLRESEERYRSIFERARDV
                     VYIIGPDQRFVSLSPAFSLATGWSPDEWIGKRFEPIVHPDDRTRAQQIFDRTLAERVS
                     SHFEMRIASRFGRYWDGDFSVGPVTLGGVTYLFGIGRDVSERKAQERRIHRLTRVHSV
                     LSGINAAIVRIHDRRQLFDEACRIAVEAGRFRMAWIGLIDERAQVVRPAASGGDVRDF
                     FDSAPLAVVDATPEGQGLVGRAVRSLAPVISNDIENDPQRLMKKECRERGILSLAVLP
                     LIVGGKAVGVLCLYSGETGFFDDEEMRLLVDLAGDISFALDHIAKAEEVAYLAYYDVL
                     TGLPNRKLFGQGLEEDLIRMRSDGSAAAVVLADVERFRNVNETFGRQAADRLLCDLAQ
                     RLVRCAGSGVRVARVEADRFALALPGVPDAAHAAHALERIMDQCFGGPFAVDGHEQLL
                     SMRAGIALFPLDGANADALYRNAEIALVKAKDATERYLFYAPEMNARVAEAVALENRL
                     RRAVEQRQFTLHYQPKVSSRTGRIVGLEALLRWNDPKTGLVPPAQFIPMLEETGLIIP
                     VGEWALREAVAQRARWTGLGLSVPRIAVNVSAIQLRRKDFVAAVKSALASQAGADHAL
                     DLEITESIIMANAEDNIEKLRELRAIGMHIAIDDFGTGYSSLRYIAQFPLDSLKIDRS
                     FVSRMPSTSGDLSIVSTIIALGHDLRLTIVGEGVENEEQARLLRALKCDEMQGYLFSE
                     PLPAPRIAALLQDPVRPVKPATTSPA"
     gene            7159..7755
                     /locus_tag="VI319_14880"
     CDS             7159..7755
                     /locus_tag="VI319_14880"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY6865178.1"
                     /translation="MSLLLAVSQAAIAKDGPIGIDHPVARDNDGLTLRRNQLLIEDGM
                     IIALVAGALRHDPASRLGDTYWRAIDASVLGAVASTGLKYVFTRSRPTESSDPNLWFQ
                     GRGHYSFPSGEVTFMSAAVTPFIMQYRDDHPAIWALELLPVYDAYARVKVGGHWQSDV
                     LAGFLLGAGAGYIAATRDSPFMLQAMPRGFMLGYRARF"
     gene            complement(7752..8534)
                     /locus_tag="VI319_14885"
     CDS             complement(7752..8534)
                     /locus_tag="VI319_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6865179.1"
                     /translation="MTSAAALAAFALLAACAAHVARPDADAVRALAPTGTLRVGVYAG
                     SPTSMVRDARTGEARGVSYELGRELAARLGVPFEPVEYPRVAEVVEAIKAGRVDFTVT
                     NASPARAKEIDFTPSILALELGYLAPRGSAIASAADVDRPGVRVGVTQGSTSQATLSK
                     TLKNATLVAAPTVKAAVDLLSSGGIDVYATNKAILSEMSDQIAGSRILEGRWGLEHMA
                     IGYPKGRAAGAEYIRRFAEAAQSEGLVRRAAQRAGLRGTAEP"
     gene            complement(8531..>8718)
                     /locus_tag="VI319_14890"
     CDS             complement(8531..>8718)
                     /locus_tag="VI319_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865180.1"
                     /translation="EALRRLSDETLRALEDRVVRDKLDALGAVVAPQASEDFGRFLAQ
                     EIRTWEAVIRKSGIHAD"
BASE COUNT         1329 a         3036 c         3036 g         1317 t
ORIGIN      
        1 gtgctccgcg cagggctacc cggcgaagcc ggtgctcgtc gtgatgccgc tgcaggcggg
       61 cagcgccgtc gacgtcatga tccgcatcgt cgcccagcgc atgagcgaga acctcggcca
      121 gcagatcctg atcgagaacc agcccggcgc ggcgggctcc atcggcgccg agcgcgtgaa
      181 gcgcgccgcg cccgacggct acaccgtcgg cgtgctgaac gattcgatcc tcaccatgat
      241 tccgaacatc cggcacgtcg gctacgaccc tacgaaagac ttcgagccgg tcggcgtggt
      301 ggctgcgatc acgtgggtca tggtggtcaa caacgacgtg ccggcgaaga cggtgcgcga
      361 attcatcgac tacgcgaagg cccggccggg gaagatcgac tactcctccg gcggcaacgg
      421 cagcccgcag catgtcgcga tggaggcatt caaggcggcg accggcctcg acctcgtgca
      481 catcccgtac aagggcgcga cgcaggccgc gatcgacatc atgtccggcc gcgtgcaggc
      541 gcacttcggc gccgtgtcga tcgtgcagcc gtacgtgaag gacggccgca tgcgcgcgct
      601 gggcgtgccg agcgcggcac gctcgccgct cctgcccgaa gtaccgacca tggccgaggc
      661 gggcgtgccc ggcttcgagt ggcgcacctg ggcgtcgttc gtcgtgccgc tgcgcacgcc
      721 caagcccgtc gtcgaccggc tgaacgccga gctcgtgcgc gcgatcaagg ccccggacgt
      781 gcgcgacaag ctgatcgcgc aggggctgga gccggtcggc tccgggcccg agacggtcac
      841 gcgctggacg cgcgacggcc tcgcgcgcat gcgcgacatc gtcaagcgcg cgaacatcca
      901 ggcggagtga gcagggtgct gacgcgcatc gatcacgtca cgatcggcgc gcggaatctc
      961 gacgaaggca tcgacgccta ccgcaggatc ggcttcgacg tcgacgacca cggcgcggct
     1021 ttcgccgaag acgacggcct cgtcctcgtg ggcggcggag aggggctgag atcgatcgcg
     1081 ctccggagcg acgacggcac tttcgcgcgc gcgccggcgc aatggatcga catcgtcccg
     1141 catcggccag tccccgcccg ccgtcatccg aaccgagtgc agcgcatcga gcgcgtctac
     1201 atcgccgtga ccgacctcgc cgccgcggtc gagaaggtcc gaagcgctct cggcttcgcc
     1261 gagccgaaga tggagcgcgg cacggtcatc gacgcggaca tggcgatatt ccagctcggg
     1321 accaccggcc tcggtctcgc gcagcccgcg ggcccgggcg tgtgcgccga cgcgctgcgt
     1381 tcgcgggggc cggggccatt ccaggtgctg ttccgcacgg gcagcatgga tgcggccgcg
     1441 aaatggatcg ccgatcacgg cgttccgccc ccgaagcgcg gcgtccgcaa cacgggcgag
     1501 caggcgatgc tcgtgacgcc cgagcacgcc tgcggcgttt acatcgggtt cgtgggtccc
     1561 gcatagctcg ggtgccggga aaacccggac gcgtcgcgcg cgagaattcc ggacgccggc
     1621 gcgcttcggc gcgcaaaatt tcacgaagtc atgcacggcc accgtattag ggggaacccc
     1681 atagcggggt tgtcagtcag cattcagaat acgcgcctgt agcagcgttg ctacggccct
     1741 gggggggagg ccgggtgggg actgagcaca tcaaagtcct cgtggtcgag gacgtcgaaa
     1801 tggacgccga gctcgcgctg cgggagctga agcgcgcggg cctgcgttgc gtcgcccggc
     1861 gcgtcgatac cgaggacgct ttccggcgcg agctcggcga attccggccc cacgtcatca
     1921 tctccgattt ctccatgccg cgcttcgacg gcatggaagc attcgccatc gcgcgcgagg
     1981 tcgaccccga cgtcccgttc atcttcgtct cgggcacgat cggcgaggag tacgccgtgc
     2041 gcgccctgaa gaacggcgcg accgactacg tgctcaagac caacctcatc cggctgccgc
     2101 cggcggtgga gcgcgccgtg cgcgacgccg aggcgcgcgt cgaggcgcgc cggatcgagg
     2161 acgcgcgccg caagatcgag cgccggttcc aggcgctgat cgagaacagc gccgacgcca
     2221 tcgttctcat cgaccccgag ggcgcggtgc tgtaccgcag tcccgccacc gggcggctgc
     2281 tgggctacac cgacgaggag cgcgccggac ggccggtgat cgagctgacg caccccgagg
     2341 acgccgcggc gctgaaggcg aagctcggcg aatctctcaa cgccccgggg aagccgatcg
     2401 ccttccagtg ccgcgcgcgc cacaagaccg acggctggaa gatcctggac ggctcgttca
     2461 acaacctcac cgccgacgac gccatcgcgg cggtggtgat caactgccgc gacatcaccg
     2521 cgagcaagga agccgagcag cgcctcgcgt acctcgctca gttcgatccg ctcaccggcc
     2581 tgccgaaccg cgacctgttc aaggaccgcc tggagcagac gctcgcgcag gcgcggcgca
     2641 acgactggct cgtcggcgtc gtgctggtga acctggatcg cttcaagatg gtgaacgaca
     2701 cgctcggcgt cggcgccggc gacgcgctgc tcacgcaggc ggcgcaacgc ctgggcgata
     2761 ccctgcgcgg cggcgacacc gcggcgcgcc tgagcggcga cgagttcgcg atcgcgctct
     2821 ccaacctcgc gcgcgcggac gacgcggcga ccgccgcgca gaaggtcctg gatgctctcg
     2881 ggcgcccctt cgaggtcggg ggacgccagg tctacgtctc ggcaagcgtg ggcgtcgcgc
     2941 tctaccccga ggacggcgcc gccggcgacg tgctgctgaa gaacgcgaac gcggccaagg
     3001 accgggtgaa ggagcagggg ggcagcggct accagttcta cctgcccgag atgaacgagc
     3061 gcgcggcgca gcgcctgcag ctcgagacgg cgctgcgcgg agcgctcgag cggcgcgagt
     3121 tcgagctgca ttaccagccg aaggcgagcc ttgcgacggg cgcgatcagc ggcttcgagg
     3181 cgctcctgcg ctggacgcaa tcggaccggc gcctcgcgtc gcccgctcgc ttcgtcccga
     3241 tcctggagga taccgggctc atcatgccgg tcggggactg ggtgctcgcg accgcatgcg
     3301 cccagatcag ggcgtggcag cacaggggcc tgggcagccg ctcggtggcg gtgaacctct
     3361 cggcgcggca gtttcagcgt cccggcctcg acgccaccat cgcgcggctg atcgaggaga
     3421 ccgggatcga cccgcgtcag ctcgagctcg agctgaccga gaccctgctg atgcgcgacg
     3481 cggagcaggc gatccgggtg ctgcggacgc tgaagtccta cggcgtgcgg atctcggtcg
     3541 acgatttcgg cacgggctac tccagcctgg cctacctcaa gcgtttcccg ctcgacgcgc
     3601 tcaagatcga ccgcgcgttc gtgcacgacg tcaacaacga tgccgacgat gccgcgatca
     3661 cgatcgccat catcaatctc gcgcacagcc tgaagctgaa ggtcatcgcg gagggcgtcg
     3721 agacgcaggc ccagctccac ttcctgcgcg agcacggctg cgacgagatc cagggctact
     3781 tcttcgagcg cccgcaacca gccggggatt gcacgcgcct gctgcaggga cggcggacgc
     3841 tgggctaaga cccgcccgca cggcgcgcgg ggcggcggtg tcgggagatt cccgacaagc
     3901 gagagctgtt cgcgcgagcg cggcgctacc ggcggcgatt ttgcgctgga tcaaaaccgc
     3961 aagcgccgtt gctgccagac tcgcggcgca tgacttcgtc gttgatcctc ccaggggagc
     4021 ggcgtctggc gcgggtgctc gcaatcctcg cggggttcgc cgcgctgttc gccgtcgtgt
     4081 ggcaggcgcc gccgcaccac gccttggaag gtatttccag ctacctgccg cttcacaacc
     4141 tgctcgaggg cgcctcgatc gtggtcgcca tgctggtctt cggcgtcacc tggaacgcgt
     4201 acacgcgcga gcgctccggg aatacggtga tcctcgcctg cggcctgctg gcggtcggcc
     4261 tgatcgactt cgcgcacatg ctctcgttca agggcatgcc ggacttcgtg acgccggccg
     4321 acccggagaa ggcaatcgag ttctggctcg ccgggcgcac ggtcttcgcg gcctgccttc
     4381 tttccgcggc ggtgcgggcc ccggcgccgc tcgcgtcgcc ggcgacgcgc tacgcgctgc
     4441 ttctcggcgc gctcgccgtc accgcgctcg tctattggat ggtcctcttg cacggcgacc
     4501 ggctgccgca cactttcatc gagggtaagg gcctcacgcc gctgaagcgg gccatcgagt
     4561 acgccattgt cgtcctgctc gcgattccgg cgctgctgtt ctacgcgaag gcgcggctga
     4621 ccgcgtcggg cgacgcggcg ctgctcttcg ctgcggcggc gatctccatc ctgggcgagc
     4681 tgagcctgac gctgtattcc gcggtcaccg acgtgttcaa cctgctcggc cacgtgtaca
     4741 agatcgcggc gttcgcgttc atctaccgcg cggtcttcgt cgcgagcgtg cgccagccgt
     4801 tcgagcgtct gcgcgagagc gaggagcgct accgcagcat cttcgaacgc gcccgggacg
     4861 tggtctatat catcggcccg gaccagcgct tcgtgtcgct gagccccgca ttctcgctcg
     4921 ccaccggatg gtcgcccgac gagtggatcg gcaagcgatt cgagccgatc gtgcatccgg
     4981 acgaccggac gcgcgcgcag cagatattcg atcgcacgct cgcggagcgc gtgtcgtcgc
     5041 atttcgagat gcgcatcgcg agccggttcg gacgctactg ggacggcgac ttcagcgtgg
     5101 gccccgtgac gctcggcggc gtcacctacc tcttcggtat cgggcgcgac gtcagcgagc
     5161 gcaaggcgca ggagcgccga atccaccgcc tcacgcgggt acattcggtg ctctcgggca
     5221 tcaacgccgc gatcgtgcgc atacacgacc gtcggcagct gttcgacgag gcctgccgca
     5281 tcgcggtcga ggccggtcgc ttccgcatgg catggatcgg gctgatcgac gagcgtgcgc
     5341 aggtcgtgag gccggccgcg tccggcggtg acgtgcgcga cttcttcgac tcggccccgc
     5401 tcgcggtcgt cgatgcaacg cccgaaggac aagggctggt gggccgcgcc gtgcgcagcc
     5461 tcgcgcccgt gatctcgaac gacatcgaga acgacccgca gcgcctgatg aagaaggagt
     5521 gccgcgagcg cgggatcttg tcgctcgccg tgctgccgct gatcgtcggc ggtaaggccg
     5581 tcggcgtgct atgcctgtac tcgggcgaga ccggcttttt cgacgacgag gagatgcggc
     5641 tcctcgtcga cctcgcgggc gatatctcgt tcgcgctcga ccacatcgcc aaggcggagg
     5701 aggtggccta tctcgcgtac tacgacgtgc tcacgggcct gccgaaccgc aagctcttcg
     5761 gccagggcct ggaagaggat ctcatcagga tgcgcagcga cggctcggct gcggcggtcg
     5821 tcctggccga cgtcgagcgc ttccgcaacg tgaacgagac cttcggtcgc caggccgccg
     5881 accggctcct gtgcgacctc gcgcagcggc tcgtgcggtg cgcgggatcc ggcgtccgcg
     5941 tcgcgcgggt cgaggcggac cgcttcgcgc tcgcgctccc gggggtgccc gacgccgcgc
     6001 atgccgcgca tgcgctcgag cgcatcatgg accagtgctt cggcgggccg ttcgcggtcg
     6061 atgggcacga gcagctgctt tcgatgcgtg cgggcatcgc gctctttccc ctcgacggcg
     6121 caaacgccga cgcgctgtac cggaacgccg agatcgcgct cgtcaaggcc aaggacgcga
     6181 ccgagcgcta cctcttctac gcgcccgaga tgaacgcgcg ggtggcggag gcagtcgcgc
     6241 tcgagaaccg gctgcgccgc gcggtcgagc agcggcagtt cacgctgcac tatcagccta
     6301 aggtcagctc gcgcaccggc cgcatcgtcg ggctcgaggc gctgctacgc tggaacgacc
     6361 cgaaaaccgg cctggtcccg cccgcgcagt tcatccccat gctcgaggag accggcctca
     6421 tcattcccgt cggcgagtgg gcgctgcgcg aggccgtcgc gcagcgcgcg cgctggaccg
     6481 gactgggcct gagcgtgccc cgcatcgcgg tgaacgtgtc ggccatccag ctgcgccgca
     6541 aggacttcgt tgcggcggtg aagagcgcgc tggcttcgca ggccggcgcg gatcacgcgc
     6601 tcgacctcga gatcaccgag tccatcatca tggcgaacgc cgaggacaac atcgagaaac
     6661 tgcgggagct gcgcgccatc ggcatgcata tcgccatcga cgactttggc accgggtact
     6721 cgtcgctgcg ctacattgcg cagtttcccc tcgactcgct gaagatcgac cgctctttcg
     6781 tctcgcgcat gccgtcgacc tcgggcgatc tctcgatcgt ctcgaccatc atcgcgctgg
     6841 gtcacgacct gcggctcacg atcgtcggcg aaggcgtgga gaacgaggag caagccaggc
     6901 ttctgcgcgc gctgaaatgc gacgagatgc agggctacct gttcagcgag ccgctgcccg
     6961 cgccccggat cgcggcgctc ctgcaggatc cggtgcggcc ggtgaagccg gccacaacct
     7021 cgcccgcatg acgcggcccg tccgtcccgg cgacggcgtc ctgcaagccg aggtcagctt
     7081 tccgcaagca gcgctgccca gaaaccgccg gcccattaca catcgaggat gatctcagtg
     7141 cgcctctgtc tgtcattgtt gtccctgctg ctcgccgtat cgcaggccgc gatcgcgaaa
     7201 gacggcccga tcgggatcga ccacccggtc gctcgcgaca acgacgggct gacgctgagg
     7261 cgcaaccagc tcctcatcga ggacgggatg atcattgcgc tcgtcgcggg agcgctgagg
     7321 cacgatccgg caagccgcct cggcgacacg tactggcgcg cgatcgatgc gtcggtcctc
     7381 ggggcagtgg cgtcgaccgg cctcaagtac gtgttcacgc gctcgcggcc gaccgagagc
     7441 agcgacccga acctctggtt ccagggccga ggacattaca gcttcccgag cggcgaggtc
     7501 accttcatga gcgcggccgt gacgccgttc atcatgcaat accgggacga ccacccggca
     7561 atctgggcgc tcgagctgct gcccgtgtat gacgcgtacg cgcgcgtgaa ggtcggcggg
     7621 cactggcaga gcgacgtgct cgccggattc ctgctcggcg cgggggccgg ctacatcgcc
     7681 gcgacgcggg acagcccgtt catgctgcag gcgatgccgc gcgggttcat gctcggctac
     7741 cgggcgcgct tctaaggctc ggccgtcccg cgcagtcccg cgcgctgcgc cgcgcggcgc
     7801 accaggccct ccgattgcgc ggcctcggcg aagcgacgga tgtactcggc gccggccgcg
     7861 cgccccttcg gatagccgat cgccatgtgc tcgaggcccc agcggccctc gaggatgcgc
     7921 gagcccgcga tctggtccga catttccgag aggatggcct tgttggtcgc gtagacgtcg
     7981 atgccgccgc tcgagagcag gtcgacggcg gccttgacgg tcggcgcggc gacgagagtc
     8041 gcgttcttca aggtcttcga gagcgtcgcc tgcgaggtgc ttccctgcgt gacgccgacg
     8101 cgcacgcccg ggcggtcgac atcggccgcc gatgcgatgg cggagccgcg cggcgccagg
     8161 tagccgagct cgagcgcgag gatggacggc gtgaagtcga tctccttcgc gcgcgccgga
     8221 gacgcgttcg tgaccgtgaa atcgacccgg ccggccttga tcgcctcgac cacttcggcg
     8281 acgcgcggat attcgaccgg ctcgaacggc acgccgaggc gcgccgccag ctcgcggccg
     8341 agctcgtagc tgacgccgcg cgcctcgccg gtgcgtgcgt cgcgcaccat cgaggtcggg
     8401 ctgcccgcgt agacgccgac gcgcagcgtt ccggtcgggg cgagggcgcg gacggcgtcc
     8461 gcgtcgggcc gggcgacgtg cgccgcgcac gcggcgagaa gtgcgaaggc ggcgagcgcc
     8521 gccgcagacg tcaatcggcg tggatgcccg atttgcggat cacggcttcc catgtgcgga
     8581 tctcctgcgc gaggaaacgg ccgaagtctt cggaggcctg cggcgcgacc accgcgccga
     8641 gcgcatcgag cttgtcgcgc acgacgcgat cttcgagcgc gcgcagcgtc tcgtcggaga
     8701 ggcgccgcag cgcctcgc
//
LOCUS       DATDMW010000357         4034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1099819, whole genome shotgun sequence.
ACCESSION   DATDMW010000357 DATDMW010000000
VERSION     DATDMW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099819"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VI319_14895"
     CDS             <1..258
                     /locus_tag="VI319_14895"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HEY6865181.1"
                     /translation="RVPRENAAVARTAIENLQRDLAAATGVRGRLLRRRDDETTWMEI
                     YEDVEDGARLEAALDELARRHGVAALLSPGGGRRREVFREL"
     gene            260..1087
                     /locus_tag="VI319_14900"
     CDS             260..1087
                     /locus_tag="VI319_14900"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY6865182.1"
                     /translation="MCLIALAWRAHKRYRLVVAANRDEYFRRPAAPAGFWDDHHLVLA
                     GRDLEAGGTWLGVTLQGRFAAITNYRNPADRRSDAPTRGRLVSDYLAGAQRPREYAAA
                     VGRKATPYNGFSMLAGDLESLWFFSNRGGAPVEVARGIHALSNHLLDTPWPKVEKGKA
                     MMRAALERPFDADALLAAFDDPRTAAGEPLPDTGVGLELEERLSAVRIAPHGGYGTRC
                     TTVLAVGEDGRVEFCERTWREDGSPGGVVRYRFTLAGAQGASRRGGSRPPRTALPSR"
     gene            complement(1011..1448)
                     /locus_tag="VI319_14905"
     CDS             complement(1011..1448)
                     /locus_tag="VI319_14905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6865183.1"
                     /translation="MPSPFTIRVLDWDDAAALARPVREEVFVVEQRVPLELEWDEHDA
                     GARHAIALAANGEAIGTARLLPEGRIGRMAVRKSWRGRGVGAALMRALLDEARAAGMA
                     GIALHAQTHAAGFYRRFGFSARGEPFEEAGIPHVEMRLALRPA"
     gene            1490..3460
                     /locus_tag="VI319_14910"
     CDS             1490..3460
                     /locus_tag="VI319_14910"
                     /inference="COORDINATES: protein motif:HMM:NF006196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEY6865184.1"
                     /translation="MRSLWNDEEAKQFKGDLGLRVYTSRLLGRDKSLVLHGGGNTSVK
                     LREKDLFGEEQAILYVKGSGWDLETIEPQGFSPVRLAHVLKLAELPALSDPEMVNQLV
                     THMLRASAPAPSIETILHGLMPQKYVDHTHADAVLSVTNTKDGDKRIREIYAKRCVVI
                     PYLMPGFDLAQFCARELKRQGTPETVGMVLMNHGIFSFGETARESYERMIELVGLSQK
                     YLESRRAWTVVPEGAATAKVDPAAQARLRRAISDAAGSPLVLRTVTDGRTLAFARHPD
                     LARISQQGPATPDHVIRTKCTPMLGGDVGRYVDGYRRYFAEHAPKAREPKTILDPAPR
                     LVLDAEFGLAAAGKTAKDAAIAAEIYDHTIDVILRAEALGGFRALPAKDLFDVEYWDL
                     EQAKLKKGGKPPAFAGEVALVTGAASGIGKAAVAAFLARGAAVVGFDLNPEIETLHRR
                     PDFLGVRGDVTSESDVAAAAAKAALAFGGIDMLVLNAGIFSASRRIADLPGDEWRKVM
                     SINLDSNLALMRTCHPYLKLAPRGGRVVVIGSKNVPAPGPGASAYSASKAALNQLARI
                     AALEWGADGIRVNTVHPNAVFDTGIWTAEVLAERARSYGMTVDQYKTNNVLRTEVTSR
                     DVAELAAEMCGPLFAKTTGAQVPVDGGNERVI"
     gene            3444..>4034
                     /gene="mtnA"
                     /locus_tag="VI319_14915"
     CDS             3444..>4034
                     /gene="mtnA"
                     /locus_tag="VI319_14915"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466012.1"
                     /note="isomerizes methylthioribose-1-phosphate into
                     methylthioribulose-1-phosphate; involved in methionine
                     salvage pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY6865185.1"
                     /translation="MSASSEATGVETLRWSGGRLELIDQRGLPRRLDYVSCGSAAEVA
                     AAIRDMVVRGAPAIGCAAAFGVALEALRHRDRRREEQDAALESAFAALAASRPTAVNL
                     FWALDRMREALAAARDAAAPAVADRLLRAAQEIMAADLATNRAIGAAGAALVPQVARI
                     MTHCNAGALATAGHGTALGVVRTARDQGKRISVIASE"
BASE COUNT          593 a         1446 c         1452 g          543 t
ORIGIN      
        1 agggtgccgc gcgagaacgc ggccgtggcg cgcacggcga tcgagaacct ccagcgcgat
       61 ctcgcggccg cgaccggggt ccggggccgg ctgctgcgcc ggcgcgacga cgagacgacg
      121 tggatggaga tctacgagga cgtggaggac ggcgcgaggc tggaggcggc gctcgacgag
      181 ctcgcgcgcc ggcacggcgt cgccgccctg ctctccccgg gcggcggccg ccgccgcgaa
      241 gtcttccgcg agctctagca tgtgcctgat cgcgctcgcg tggcgcgcgc acaagcgcta
      301 ccggctcgtc gtcgcggcga accgcgacga gtatttccgc cggccggcgg cgcccgcggg
      361 gttctgggac gatcaccatc tcgttctcgc gggccgcgac ctggaggcgg gcggcacctg
      421 gctgggcgtt acgctgcaag gacgctttgc cgcgatcacg aactaccgca atcccgccga
      481 tcgccgctcc gacgcgccca cgcgcgggcg gctcgtgagc gattatctcg cgggcgcgca
      541 gcggccgcgc gagtacgcgg ccgccgtcgg ccgcaaggcg acgccgtaca acggcttcag
      601 catgctcgcc ggcgacctcg aatcgctatg gttcttctcg aaccgcggcg gcgcgccggt
      661 cgaggtcgcg cgcggcattc acgcgctgtc gaaccacctc ctcgatacgc cctggccgaa
      721 agtggagaaa ggcaaggcga tgatgcgcgc ggcactggag cgtcccttcg acgcggacgc
      781 gctgctcgcg gcgttcgacg atcctcgcac ggccgcgggc gagccgctcc ccgacaccgg
      841 cgtgggcctg gagctcgagg agcgcctctc cgccgtgcgc atcgcaccgc acggcggcta
      901 cggcacgcgc tgcacgacgg tgctcgccgt cggcgaggac ggccgggtcg agttctgcga
      961 gcggacgtgg cgcgaggacg gcagcccggg aggcgtcgtg cgctaccgct tcacgctggc
     1021 cggagcgcaa ggcgcatctc gacgtggggg atcccggcct cctcgaacgg ctctcccctc
     1081 gcgctgaagc cgaaccggcg atagaagccc gccgcgtgcg tctgcgcgtg cagcgcgatc
     1141 ccggccatgc cggccgcgcg cgcctcgtcg agcagcgcgc gcatcagcgc cgcgccaacg
     1201 cctcggccgc gccaggactt gcgcaccgcc atccgcccga tgcggccctc ggggagcagg
     1261 cgcgcggtgc cgatcgcctc gccgtttgcg gcgagagcga tcgcgtggcg ggcgcccgcg
     1321 tcgtgctcgt cccactcgag ctccagcgga acgcgctgct cgacgacgaa aacctcctcg
     1381 cgcacgggcc gggcgagcgc ggcggcatcg tcccagtcga gaacgcggat cgtgaacggc
     1441 gaaggcatgc gagaccttat aatcgagggc attttacgca aggacggcga tgcgaagcct
     1501 ctggaacgac gaggaagcga agcagttcaa aggcgacctc ggactccgcg tctacacctc
     1561 ccgcctgctc ggacgcgaca agtcgctcgt cctgcacggc ggcggcaaca cctcggtcaa
     1621 gctgcgcgag aaggatctct tcggcgagga gcaggcgatc ctctacgtga agggcagcgg
     1681 ctgggacctc gagacgatcg agccgcaggg attctcgccg gtgcgcctcg cgcacgtgct
     1741 caagctcgcc gagctgcccg cgctctccga tccggagatg gtcaatcagc tcgtcaccca
     1801 catgctgcgc gcgtcggcgc ccgcgccgtc gatcgagacc atcctgcatg ggctgatgcc
     1861 gcagaagtat gtcgatcata cgcatgccga cgccgtgctc tcggtcacga acacgaagga
     1921 cggcgacaag cgcatccgcg agatctacgc caagcgctgc gtcgtgatcc cctatctcat
     1981 gcctgggttc gacctcgcgc agttctgcgc gagggagctc aagcgccagg gaacgcccga
     2041 gacggtgggg atggtgctca tgaaccacgg catcttctcc ttcggcgaga ccgcgcgcga
     2101 gtcctatgag cggatgatcg agctggtggg gctgtcgcag aagtacctcg agtcgcgccg
     2161 cgcttggacg gtggtgcccg agggcgccgc gacggccaag gtggatcccg cggcgcaagc
     2221 gcgcctgcgc cgcgcgatct ccgacgcggc gggctcgcct ctcgtccttc gcaccgtgac
     2281 cgacgggcgc acgctcgcgt tcgcgcggca cccggacctc gcgcgcatct cgcagcaggg
     2341 gccggcgacg ccggaccatg tcatccgcac caagtgcacg cccatgctcg ggggcgacgt
     2401 cggccgctac gtcgacggct accgccgcta cttcgcggag cacgcgccca aggcgaggga
     2461 gccgaagacc atcctcgatc ccgcgccgcg cctggtgctc gacgcggagt tcggcctcgc
     2521 cgccgcggga aagaccgcga aggacgcggc gatcgcggcc gagatctacg accacacgat
     2581 cgacgtgatc ctgcgcgccg aggcgctggg cgggttccgc gcgctgcccg cgaaggactt
     2641 gttcgacgtc gagtactggg acctcgagca ggcgaagctg aagaaaggcg ggaagccgcc
     2701 cgccttcgcc ggggaggtgg cgctcgtcac cggcgcggcg tcgggcatcg gcaaggcggc
     2761 cgtcgccgcc tttctcgcgc gcggcgccgc ggtggtcggc ttcgacctga acccggagat
     2821 cgagacgctg caccggcggc ccgatttcct cggcgtgcgc ggtgacgtca cgagcgagtc
     2881 cgacgtcgcc gcggcggccg cgaaggccgc cctcgccttc ggtggcatcg acatgctcgt
     2941 gctcaacgcg ggcatttttt ccgcgagccg caggatcgcg gacctgccgg gagacgagtg
     3001 gcgcaaggtc atgtccatca acctcgactc gaacctggcg ctgatgcgca cctgccatcc
     3061 ctatctcaag ctcgcgcccc gcggcggacg cgtcgtcgtc atcggctcga agaacgtgcc
     3121 ggcgccgggt cccggcgcgt cggcgtactc ggcgtcgaag gccgcgctca accagctggc
     3181 gcgcatcgcc gcgctcgaat ggggcgccga cggcatccgc gtcaacaccg tgcacccgaa
     3241 cgccgtgttc gacaccggca tctggaccgc ggaggtgctc gccgagcgcg cgcgcagcta
     3301 cggcatgacg gtcgaccagt acaagacgaa caacgtgctg cgcaccgagg tcacgagccg
     3361 cgatgtcgcc gagctcgccg cggagatgtg cgggccactg ttcgcgaaga cgacgggcgc
     3421 gcaggtgccg gtcgacggcg gcaatgagcg cgtcatctga ggcgacgggg gtcgagacgc
     3481 tgcgctggag cggcgggcgt ctcgagctga tcgaccagcg cggccttccg cgccggctcg
     3541 attacgtgag ctgcggctcc gcggcggagg tcgccgccgc catccgcgac atggtggttc
     3601 gcggggcacc cgcgatcgga tgcgctgcgg ccttcggggt cgcgctcgag gcgctgcgtc
     3661 accgcgatcg tcggcgcgag gagcaggacg ctgcgctcga gtccgccttt gccgcgctcg
     3721 ccgcgagccg accgaccgcg gtcaacctct tctgggcgct ggatcgcatg cgcgaggcgc
     3781 tcgcagcggc gcgggacgca gcggcgcccg ccgtggccga tcgcctcctg cgcgccgcgc
     3841 aggagatcat ggcagcggac ctcgccacca accgcgcgat cggtgccgcc ggcgcggcgc
     3901 tcgtcccgca ggtcgcgcgc atcatgacgc attgcaacgc gggggcgctc gcgaccgccg
     3961 ggcacggcac ggcgctcggc gtggtgcgca ccgcgcgcga ccagggcaag cgcatctcgg
     4021 tgatcgcgag cgag
//
LOCUS       DATDMW010000358         4982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2346168, whole genome shotgun sequence.
ACCESSION   DATDMW010000358 DATDMW010000000
VERSION     DATDMW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2346168"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1004
                     /locus_tag="VI319_14920"
     CDS             336..1004
                     /locus_tag="VI319_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865186.1"
                     /translation="MKIALDMNKPRKIAFGALAAVAALAFWPQNDRPIAAPREPSAPA
                     APAPQPRTAIAQVETLPARDAMPQLARDPFSPEPPKKPVRTGVVTAPAAPVNPYRFAG
                     EMRVGDSTQRFLARGDDTFEAKAGDALEDGYAVESVSAAAIVLVHRPSGVKQTLAVGA
                     PAWEDAARSRVMQAAQPAPAAVASSDAPRPDMMRLDALSGSLPQGFFVDVGGVPAARQ
                     PGSG"
     gene            1161..1964
                     /locus_tag="VI319_14925"
     CDS             1161..1964
                     /locus_tag="VI319_14925"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEY6865187.1"
                     /translation="MLRKLCLLGAAVGALSTGAIAQTPEVTLTRLDCGTGFNDQRRFS
                     DTFAYTDPKMPFTFSCYVIRHGADVMVWDTGFVPGSSPNAPKVSLTQQLEQLHIKPED
                     VKYVGISHFHGDHTGQLPSLSGATLLIGQKEWDAITAPKPMAGANVPAFQHWISGGGK
                     VEPQPADKDVFGDGTVMVLRTPGHTPGHQSLLVRLKEKGPVILVGDAAHFHENYDSDG
                     VPGFNYDRAETIASMERIKGIAKNLKATVIIQHDPRDIGKLPAFPTAAK"
     gene            2044..3363
                     /gene="rimO"
                     /locus_tag="VI319_14930"
                     /pseudo
     CDS             2044..3363
                     /gene="rimO"
                     /locus_tag="VI319_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234065.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
     gene            complement(3440..4051)
                     /locus_tag="VI319_14935"
     CDS             complement(3440..4051)
                     /locus_tag="VI319_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY6865188.1"
                     /translation="MDVTFSSAVVILLLVIDPVGNIPIFVVALRGIPAKRRARIVVRE
                     CLIALGVLIVMALAGKPLLDLLGLSDRSLNIAGGVILFLISLRMIFRSADGIFGDTRV
                     AGEPFIVPLAIPAMSGPTAIATVMLFVSRAPERLFEWLLAIALAMILSIAVLVFAERI
                     SKRIGERGLVALERLMGLLLTAVAVEMLLRGIEGFVQHLRASS"
     gene            complement(4069..>4982)
                     /locus_tag="VI319_14940"
     CDS             complement(4069..>4982)
                     /locus_tag="VI319_14940"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6865189.1"
                     /translation="VIVREKQSLRYAQDDDEMTGVAIALVLGAASIHASWNYILKKSG
                     GGVGFVWAFAALSSLFYAPLAVAVSIYTGFRFPPLALACIAASAVLHTAYYLLLDRGY
                     RHGDLSVIYPLARATGPLLTIVVAIAVLGERPGPVALGGAALVVGGAFFLAASPARLR
                     QAGALRGIGFALLTGCMIASYTVVDKLSVSAVLVPPLVLDWGANLGRVIVMTPLALRR
                     RDAVLAAWRERRGPVIAVAVLCPLSYIMVLTAMAFTPVSYVAPAREISILFAALMGAH
                     WLREGDVARRVLAALAMALGVVALALG"
BASE COUNT          894 a         1697 c         1720 g          671 t
ORIGIN      
        1 cgaaagcgcg ccgagcgcaa gcgcgatcgg tgccagttgg aacaatttca tttgaagctc
       61 ccttacgtag ttgtgggggt cgattgcttc tgcttttcag cgaccgccca ttcagcgagg
      121 accatgccaa cggaatgcat cccgcacaga gcaatgattc gaaaaggcat tgctgtcgtg
      181 cgtcgcttgc cggagacgcg cgcgcatggg gcactacacc cggcattggg ggattagccc
      241 cggtccattg ccccgaaccc ccgatcgggc gcgcagaaac gcccgtgaaa acaacgaccg
      301 cgacgccggg cccacctctt gctgaagatc gccgcatgaa gatcgccctc gacatgaaca
      361 agcccagaaa gatcgcgttc ggcgcgctgg cggcggtcgc cgcgctcgcg ttctggccgc
      421 agaacgatcg gccgatcgcg gcgccgcgcg agccgtccgc gcccgccgcg ccggccccgc
      481 agccgcgcac cgcgatcgcg caagtcgaga cgctgccggc gcgcgacgcg atgccgcagc
      541 tcgcgcgcga cccgttctct ccggaaccgc cgaagaagcc ggtgcgcacc ggagtcgtga
      601 ccgcgccggc ggcaccggtg aacccttatc gcttcgccgg cgagatgcgc gtcggcgaca
      661 gcacgcagcg cttcctcgcg cgcggcgacg acacgttcga agcgaaggcg ggcgacgcgc
      721 tcgaggacgg ctacgccgtc gagtcggtga gcgcggcggc gatcgtgctc gtgcaccggc
      781 cttcgggcgt gaagcagacg cttgccgtcg gcgcgcccgc gtgggaagac gcggcgcgga
      841 gccgcgtgat gcaggccgcg cagcccgcgc cggcggccgt cgcgagcagc gatgcgccgc
      901 gtcccgacat gatgcggctc gatgcgctga gcggcagcct gccgcagggc ttcttcgtcg
      961 atgtcggcgg agtcccggcg gcgcggcagc ccgggtcggg gtgacgcggg aaaccccgtt
     1021 ccgcgtttcg gcgaagcgca ccggcaacaa cccaaaaggc accggtattc cgccgggtct
     1081 ttttcttttt cagccggatt gaccgctgcg ccccggtccg gcaacatcgg cgttcccttt
     1141 ttcgaggaag aggaaacgca atgctcagga aactctgcct gctcggcgca gccgtcggcg
     1201 ccctgtcgac cggcgccatc gcccagaccc ccgaagtcac gctgacgcgc ctcgactgcg
     1261 gcaccggctt caacgatcag cgccgcttca gcgacacgtt cgcgtacacc gatccgaaga
     1321 tgccgttcac gttcagctgc tacgtgatcc ggcacggagc cgacgtcatg gtctgggaca
     1381 cgggcttcgt gcccggctcg agccccaacg cgccgaaggt cagcctcacc cagcagctcg
     1441 agcagttgca catcaagccc gaggacgtga agtacgtcgg catcagccat ttccacggcg
     1501 accacaccgg ccagcttccg tcgctgtcgg gcgcgacgct gctcatcggc cagaaggagt
     1561 gggacgcgat cacggcgccg aagcccatgg ctggcgcgaa cgtccccgcg ttccagcact
     1621 ggatctcggg cggcggcaag gtcgagccgc agcccgccga caaggacgtg ttcggcgacg
     1681 gcacggtcat ggtcctgcgc acgccgggcc atacgccggg gcaccagtcg ctgctcgtgc
     1741 gcctgaagga gaagggtccc gtcatcctgg tcggcgacgc ggcgcacttc cacgagaact
     1801 acgacagcga cggcgtgccg ggcttcaact acgaccgcgc cgagacgatc gcctcgatgg
     1861 agcgcatcaa gggcatcgcc aagaacctga aggcgacggt catcatccag cacgacccgc
     1921 gcgacatcgg caagctgccg gctttcccga cggcggcgaa gtaatccgat tcgggcagta
     1981 cctcgagagg ccccggcgat cgccggggcc ttttcattgg catcgggcta aaatgatcga
     2041 cccgtgcctg caaatcccaa agtcggtttc gtctccctcg gttgcccgaa agcgctcgtc
     2101 gattccgagg gcatcctcac gcgcctgcgc gccgagggct acgcgatcgc gccgacgtac
     2161 gagggcgcgg acctcgtcgt cgtgaatacg tgcggcttca tcgagtcggc ggtcgaggag
     2221 tcgctcgacg ccatcggcga ggcgctcgac cagaacggca aggtgatcgt taccgggtgc
     2281 ctgggcgcga agggcgacat cgtccggcag acgcacccga aggtgctcgc cgtgacgggt
     2341 ccgcacgcgc tggaataagt gatgggcgcg ttgcacgagc acctgccgaa gccgcacgat
     2401 ccgtacaccg atctcgttcc tccggcgggg atcaagctga cgccgcggca ctacgcgtat
     2461 ctcaagatct cggaaggctg caatcaccgc tgcaccttct gcatcatccc gtcgctccgc
     2521 ggcgacctcg tcagccggcc ggtgggcgag gtgatgcagg aggcggagaa cctggtgaag
     2581 gcgggcgtgc gcgagctgct cgtcatctcg caggacacga gcgcgtacgg cgtcgacgtg
     2641 aagtaccgca ccggcttctg gggcgggcgg ccgctgaaga cgcggatgaa cgacctcgtg
     2701 gcagcgctcg gcgagctggg cgtgtgggtg cggctgcact atgtctatcc gtatccgcac
     2761 gtggacgacg tgatcccgct gatggccgag gggaagatcc tgccgtacct cgacgtgccg
     2821 ttccagcacg cgagcccgcg catcctcaag ctcatgaagc gtccggcgag cgcggagaac
     2881 aacctcgagc gcatccgcgc gtggcgcagg atctgcccgg agatcacgat ccgcagcacc
     2941 ttcatcgccg gcttcccggg cgagagcgag gccgagttcg aggagctgct cgcgttcctc
     3001 gaggaagccg agctcgaccg cgtgggctgc ttcgcgtatt cgccggtcga gggggcgaag
     3061 gcgaacgagc ttccgggggc ggtcgccgac gacgtgcgcg aggatcgccg cgcgcgcctg
     3121 atgcagctcc aggagaagat cagcgcccgc cggctgaaga agaaagtggg aaagaccctg
     3181 cccgtgctgg tcgacgaggt gacggagaag ggcgcggtcg ggcgctcgtc cgccgacgcg
     3241 ccggaaatcg acggcctggt ccatatcgag aacgggaaga agctcaaggc cggcgacttt
     3301 gtggacgtca ggatcacgcg ctccgacgcg cacgacctgg atggtgtcat tctgagggcg
     3361 tagcccgaag aatctgcttt tgcctttcgc gaaaaccgaa aagcaggtcc ttcgctccgc
     3421 tcaggacgac aggcgaagct caggacgacg cgcggaggtg ctgcacgaac ccctcgatcc
     3481 cgcgcagcag catctccacc gcgaccgcgg tcaacagcag ccccatcagc cgctcgagcg
     3541 cgaccagccc gcgctcgccg atgcgcttgc tgatgcgctc ggcgaagacg agcacggcga
     3601 tcgacaggat catggcgagc gcgatcgcga gcagccactc gaacaggcgc tcgggggcgc
     3661 gcgaaacgaa cagcatcacg gtcgcgatcg cggtcggccc ggacatggcg gggatcgcga
     3721 gcgggacgat gaagggctcg ccggcgacgc gcgtgtcgcc gaagatgccg tcggcggagc
     3781 ggaagatcat gcgcagcgag atgaggaaca ggatcacgcc gcccgcgatg ttgagcgagc
     3841 ggtcggagag cccgagcagg tcgagcagcg gcttgccggc gagcgccatg acgatcagca
     3901 cgccgagcgc gatcaggcac tcgcgcacga cgatgcgggc gcggcgcttc gccgggatgc
     3961 cgcgcagcgc gacgacgaag atcgggatgt tgccgaccgg gtcgatgacc agcagcagga
     4021 tgacgaccgc ggaggagaac gtgacgtcca tgcgcgacga ttatcgagtc agcccagcgc
     4081 cagggcaacc accccgagcg ccatcgcgag cgcggcgagc acccgccgcg ccacgtcgcc
     4141 ttcccgcagc cagtgcgcgc ccatgagcgc cgcgaacagg atcgagatct cgcgcgccgg
     4201 ggcgacgtag ctgaccgggg tgaacgccat cgcggtgagc accatgatgt acgacagcgg
     4261 gcacagcacc gcgaccgcga tcaccgggcc gcgccgctcg cgccaggccg cgaggacggc
     4321 gtcgcggcgg cgcagcgcga gcggggtcat cacgatcacg cgccccaggt tcgcgcccca
     4381 gtcgagcacg agcggcggca ccagcaccgc gctcacggac agcttgtcga ccaccgtgta
     4441 cgacgcgatc atgcagccgg tcagcagcgc gaagccgatc ccgcgcagcg cgcccgcctg
     4501 gcgcagccgc gcggggctcg cggcgaggaa gaaggcgccg cccaccacca gcgcggcgcc
     4561 cccgagcgcc accggaccgg gccgctcgcc gaggaccgcg atcgcgacca cgatggtgag
     4621 cagcggcccc gtcgcccgcg ccagcggata gatcaccgag agatcgccgt ggcggtagcc
     4681 gcggtcgagg aggaggtagt aggcggtgtg cagcaccgcg ctcgccgcga tgcacgccag
     4741 cgcgagcggc ggaaagcgga acccggtgta gatcgagacc gcgaccgcga gcggcgcgta
     4801 gaacagcgac gacagcgcgg cgaaggccca gacgaatccg acgccgccgc cgctcttttt
     4861 caggatgtag ttccacgaag cgtggatcga ggccgcgccg agcaccaggg cgatcgcgac
     4921 tccggtcatt tcgtcgtcat cctgagcgta gcgaaggctc tgcttttcgc gaacgataac
     4981 ag
//
LOCUS       DATDMW010000359        16088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2222121, whole genome shotgun sequence.
ACCESSION   DATDMW010000359 DATDMW010000000
VERSION     DATDMW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16088
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222121"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VI319_14945"
     CDS             complement(<1..407)
                     /locus_tag="VI319_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6865190.1"
                     /translation="MNPNLKIDIFPHIFPKAFFDRMMQIAEGNPALKANIKRWLHIPV
                     LWDLDARLKMMQRFRGYKQVLTLSLPAIEYLAGPEESPALARLANDGMAEICAKYPAQ
                     FPAWVASLPMNNVPAALEEIDRAIGRMGAKGIQ"
     gene            complement(444..1244)
                     /locus_tag="VI319_14950"
     CDS             complement(444..1244)
                     /locus_tag="VI319_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6865191.1"
                     /translation="MSELSDLSFVTVQGARLEVQRIAGASPDAPTLVFLHEGLGSISM
                     WRDFPRKAAEATGCAAVVYSRRGYGRSDPLRGARPVRYMHDEALEVLPRLLDDLGVRE
                     PVLVGHSDGGSIAIIYAAVQPQPKSGLKGLVVMAPHVFVEDLSVESIAQAKVAFETTD
                     LRERLARHHADPDGAFRGWNDVWLHPEFRAWNIEEHLPRIACPVLAIQGFDDEYGTMA
                     QIDAVVRQVSGPVEALRLDRCGHSPHRDQPAAVLEALARFVTRLDDGA"
     gene            complement(1285..2373)
                     /locus_tag="VI319_14955"
     CDS             complement(1285..2373)
                     /locus_tag="VI319_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEY6865192.1"
                     /translation="MRRPGTRAQFAALAVAALWLVGTAGAQVQYPAVSAGIALRFPHD
                     HGSHPRFRTEWWYVTGWLRASDGSERGMQITFFRNRPGVAEEVPGALAARQLLFAHAA
                     LADPANGRLLHDQRAARAGLGLADAREGGTDVAIGDWSLKQTANGYAADIPAREFRYT
                     LSFEAREPLMLNGDRGVSRKGPRSEQASYYYSRPHLEVVGTVTVGGRAMGVRGEAWLD
                     HEWSSEYLAPGAAGWDWVSVNLDGGGALMAFRIRDKNGAKLWAGGTVRGPDRRARVLA
                     PEEVDFTPLKRWRSPRTRADYPVAMRVRAGDVRIELQPLMDDQELDARASTGTVYWEG
                     AVRATADGKLLGLGYLELTVYWKPLKLQ"
     gene            complement(2370..3218)
                     /locus_tag="VI319_14960"
     CDS             complement(2370..3218)
                     /locus_tag="VI319_14960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6865193.1"
                     /translation="MALADLIFGLRARRGAIPDLPGFQRDAGARALHRSLQRWLPLAG
                     MLGADMDLLAALAGTDKYGLHMYTPVYAALTAARRGRSVSLLEIGVGDFGGRPGGESL
                     LMWAAYFRKGRIYGIDIVDKTALSSGRIKVFQCSQTDRERLTALARDIGPFDFVVDDG
                     SHRSEHQIESFRILWPFVKDGGVYVVEDVQTSYWPYFGGGVLGTREYGGSCMSFFKTL
                     ADSVNLPEFLEPPAPEAGLQETIGAVAFHHNMIAITKDAGPRRSNLDLAALRPVLMRP
                     RESPDP"
     gene            complement(3237..5789)
                     /locus_tag="VI319_14965"
     CDS             complement(3237..5789)
                     /locus_tag="VI319_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6865194.1"
                     /translation="MIRAAFRELLLAPMLEHKGRLALSIGAIALGVALGYSVQLVNDA
                     AISEFTQAVHTLAGDADVHVRGGAAGAGGGFDEGLYARLARLPEVAVASPVVEVDAKV
                     PGRRDALRVQGVDVFRAALIQPLAFGGEVGDRLDWLRPDRVFLSASAADALGLAPGDA
                     LALQVGLGTLTFRVAGVLPSGAIRGRVAMMDVAAAQAAFGRAGRLDRVDLRLRAGVPA
                     EAFAARLRAEVPPGVVVEPADADAERGASLSRSYRVNLNVLSLVALFTGGLLVFSTQA
                     LAVVRRRAQLALLRVLGVTRAGIVALLLGEAAVIGALGALAGLVLGQIGAEGVLRYFG
                     AELGAGYFRGLRPAVSFDAAAMAGFFLLGTATALLGSLASALEASRAQPAAALKAGDE
                     QRAFAPLRSAWPGVAMLALGAALTQAGPIRALPIAGYVSIALLLVGTIALMPRIAVVF
                     FRKLPGFRLPSAGLALAQLRGAPGPVGVSLAAIVAGVSLMVSMAIMIASFRQSLDDWL
                     ERVLPADLYVRAGLGGDSAFLAPADQRALAAVAGVRHAEFQRTRRLVLAPARPAIVLI
                     ARGVEREGRLPPLVGPARDRVPGGPPAAWPSEIAADLYGWRAGQVIELPIPLADKAPK
                     PGHVGPHGLPAFVVAGVWRDYARQQGAVLIERDVYIALTRDASANEAALWLAPGASVD
                     AVERALSGIPGGANLELARPGELRDVSLRIFDRTFAVTYALEAAAVILGLLGLASSFG
                     ALVLARRREFGMLRHVGMTRAPIGAMLGLEGVLVSALGLAAGLALGWLVSLVLIDVVN
                     RQSFHWSMDLHVPWTSLAVFALAMLGLSTVTALASGRRAMSREAVLAVKEDW"
     gene            5858..7042
                     /locus_tag="VI319_14970"
     CDS             5858..7042
                     /locus_tag="VI319_14970"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY6865195.1"
                     /translation="MPPLQLEPRLAGILSAEYPRFSDAEMARRREAIERVMRAAEVDH
                     LLVCGEQRAGTGVGWLTSWSATTEAIVIVAPPERQVMVMEWYNHWPLAKLLARGTEVR
                     WGEHRGIDKVIADLKTRKVRRLGFMGFLGLSKLRKLEAVFGPLVDLNAEYVKLRLVKS
                     KEEIDWMRIGCALTDLAIDALRREARAGMSERELGAICEAAYHPHGGWTFIHYFMATP
                     MDDPQYCVPRQIASSRRIRKGDAIATEITAHFFDYPGQVLRSFTVDAEPTPLFRRLHE
                     TAEAAFDAVTGVIRHGTTMQQIIDAAGVIEDAGFTVYDDLMHGFGGGYFPPVLGSRSR
                     PAGPLPHLTLQSGMTCVVQPNVITPDQRAGVQVGELILVTDTGFERMHRAERGFFRIS
                     GA"
     gene            7128..7472
                     /locus_tag="VI319_14975"
     CDS             7128..7472
                     /locus_tag="VI319_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865196.1"
                     /translation="MRIYLLVPLHTSDPIWMFSLQREAVQVIAPSETEARLRASLRYG
                     RRRTREGAVSPRDPWLDAKWVYAHVIEHTDAAMPVLAWEPVPIQPTASRADPLPSLLQ
                     RSDEPRDDQPRS"
     gene            complement(7482..8483)
                     /locus_tag="VI319_14980"
     CDS             complement(7482..8483)
                     /locus_tag="VI319_14980"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865197.1"
                     /translation="MNKRRTLLAVAALHALLGPLAAIAQSAAPARRIKRIGFLALRSR
                     PASWTNDYYGSFLRRLAQLGYVEDENVVFDWRFADGKPERLPELAKGLVQGAPDVIVT
                     SGTGATRAVQRETTTIPIVMAVAADPATSGFITNLARPERNVTGVARAPVEVSPRYLE
                     LLMAFAPKLERVAVLLNPDNEFYSPRLKSILEVARAANVRVQRVEARTAGEIESGFRV
                     MSAEGAQGVIVPFDPIYVQYRRELAVAALGNRLPSVFGVREHVTAGGLMSYGQDFNQL
                     YRRAAVYVDRILRGAKPSELPVAQPSRFELVINLKTAKALGLEIPESLRKNATRLVG"
     gene            complement(8574..9248)
                     /locus_tag="VI319_14985"
     CDS             complement(8574..9248)
                     /locus_tag="VI319_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6865198.1"
                     /translation="MAEAPLLSLRGVSKAFGASRAVLRGVDFDLAAGEYVAVMGESGV
                     GKSTLLNLIAGLDRPDEGTIAFEGAELLALGDDARTRLRRRKMGFVFQAFHILPYLTV
                     AQNVGLPLLLNGVPAAEAGGRVAAMLRAVRLDERAQSLPRELSGGETQRVAIARALVH
                     GPRLVLADEPTGNLDPETAATVLALLRERVKGEGSAGILVTHSAAAARTADRICVLCE
                     GGLRPA"
     gene            complement(9241..10005)
                     /locus_tag="VI319_14990"
     CDS             complement(9241..10005)
                     /locus_tag="VI319_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6865199.1"
                     /translation="MAERPALATRALTRHFGGLTAVREVSIECRVGEVHAIIGPNGAG
                     KSTLINVLSGELRPSAGAVELDGDDVTGLPPHRMSRLGVGRSYQKTNVFLPFTAVENC
                     RIAAQARLASSMRFLRPALANRAVNEAAERALAAAGLEARAETPVAALSHGEQRQVEI
                     AMVLATSPRVLLLDEPLAGMGAEESARIVELLRRLAPDHAVLLVEHDMDAVFAVARRL
                     TVMVNGAVLESGPPETIRASRAVREAYLGTEEPVRG"
     gene            complement(9998..10984)
                     /locus_tag="VI319_14995"
     CDS             complement(9998..10984)
                     /locus_tag="VI319_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6865200.1"
                     /translation="MGMSRSALALALCVAALALVPLAAETFYVQFFAKIMIMAIFAMS
                     LDLLLGYAGLVSFGHAAFYGAAGYALALTTPQYQAASFWTSLPLAVAASALLALAIGF
                     FVVRTRGIYFIMVTLAFAQMLFFLFYDTKIAGGNDGIYIDVRPVADLAGWRPFSLDDY
                     RHVYWLALAFLAAVYLLLRAVLRSPFGRVVSGIRVNEQRMISLGYPTFRYKLACFVIS
                     GAIAGIAGYLAAVQFGVVNPETMGWHLSAAVMMMVILGGMGTLLGPVLGAAAWMLLEL
                     GLQGLPEAGGVNLGKHWQMFMGLFIVVATLVLPHGLVGLARSLRRPAERNNG"
     gene            complement(10984..11880)
                     /locus_tag="VI319_15000"
     CDS             complement(10984..11880)
                     /locus_tag="VI319_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6865201.1"
                     /translation="MDLHTFLIQLLNGVQYGLLLFLVASGLTLVFGIMGIINVAHGSF
                     YMIGAYLAWSLTARLGSFWIAVPAAIVITAAVGVLLEWLVIRRLYGRDHLYQVLLTYG
                     LILVFEEMRSLLWGDDVRGVPVPASLDASIPLSDTLSYPVYRLFISGVCLAVALLIVW
                     VIQRTRLGMTIRAGAVDRQMVQSLGIDVHALYRVVFAGGITLAAFAGMVSAPVSAIAP
                     GMGNPVLVLSFVIIVIGGMGSVKGAMLASLLIGLVDTFGKVMHLEIAGVRVLPELSSM
                     TVYLLMAVVLLWRPEGIFGRRT"
     gene            complement(11941..13128)
                     /locus_tag="VI319_15005"
     CDS             complement(11941..13128)
                     /locus_tag="VI319_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865202.1"
                     /translation="MFDRRGFIKGAGVLGAGLAFPGLVLGQGAKIKVGLMLPYTGTYA
                     PLGVAIENGFRMALGESGGRLGGREVEFYKVDDESEPAKATDNVNRLVTRDKVDVVLG
                     TVHSGVAAGMIRVTKESGTLHIIPNAGVGAATGPLCAPNIVRTSFSNWQSGYAMGAVL
                     AQRKQIRNVVTLTWRYAAGEEFVKGFKDGYAKGGGTIAKELWLPFPNVEFQALLTEIA
                     AARPDAVYSFFAGGGAAKFLRDYAQAGLKDKIPLVGPGFLTDGVLDAAGEAAQGIETC
                     LHYADSLNTKRDNDFRAAYAKAHKMQPDVYAVQGYDSGLLLAAALKSVGGRIEDKKAV
                     VAAMEKVTVDSPRGKWHLSKAHNPVQDIYLRRVSGKENKYVGVAVKALDDDPASIAAC
                     KMS"
     gene            complement(13174..14523)
                     /locus_tag="VI319_15010"
     CDS             complement(13174..14523)
                     /locus_tag="VI319_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY6865203.1"
                     /translation="MPTVASLARDLAEGRTSSRELIERALARIADPAGEGARTFIRVY
                     AERARADADACDRSRKAGAVPSPLAGIPVSVKDLFDVAGDVTTAGSKVLRDAAAASAD
                     AVAVARLRAAGAVVIGRTNMTEFAYSGIGINPHYGTPANPWDRATRRIPGGSSSGAAV
                     SVADGMAVVGLGTDTGGSVRNPAALCGVAGFKPTQRRVPLAGCFPLSGSLDSIGPLAP
                     SIACCATAFQVLAAEPVRALEAAPPAGLRLGVPKNVVLDDLDPEVARAFEAALARLAS
                     RGARLVDMRLPELDEIAAANRRGGILAPEAYALHRKRLAERGADYDPRVRRRLEAAKD
                     VPAADYVDLLQLRSRLVSGFRRERHGVDALLLPTVPKIAPAIEPLERDMERFFAVNPL
                     TLRNTSPFNFLDTCAASLPIQRPGEAPVGLTVAGFPGEDERVLVIGLTIEAALGGPA"
     gene            complement(14526..14915)
                     /locus_tag="VI319_15015"
     CDS             complement(14526..14915)
                     /locus_tag="VI319_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEY6865204.1"
                     /translation="MAIQKEHKEFHTVDLGKGWQTPEGYPSGIQQQILAGFLDERAKR
                     GFRTRHLRFAPGVYTTEPFVHDYWEEVYLVSGDLVVGCDRNGKGGTKYGPNTYACRPP
                     GTPHGPFRSDSGCLLLELHYYDEGAKR"
     gene            complement(14930..15607)
                     /locus_tag="VI319_15020"
     CDS             complement(14930..15607)
                     /locus_tag="VI319_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HEY6865205.1"
                     /translation="MAELAFRIEGRERALRVERLVIAGWTGRDRAAVDHHIAELAAIG
                     VKPPRAVPSFYRVGASLLTTAPSIEVVGGDSSGEVEFVLVSASDGVWVGVGSDHTDRK
                     VESYGVTVSKQMCPKPIGPELWRLADLEDHWDRLVLRSHVVRAGRRLLYQEGPVSRML
                     APRELIARMPASPGALPAGTAMFCGTLPVQGEIGGGERFEIELEDPVKKRALAHAYAV
                     TKLEIAD"
     gene            complement(15609..>16088)
                     /locus_tag="VI319_15025"
     CDS             complement(15609..>16088)
                     /locus_tag="VI319_15025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY6865206.1"
                     /translation="LTTDARPTGVTVNSFGSVSLEPALVLFSLARRAHIYEVFARAPA
                     FTVNVLSQKQKLVSNRFTRPGSLVWDDIVHHVGANGCAVLADSLAWFECERRVEHDGG
                     DHVIFVGHVTAFGVSGEEADPLVFYRGNYGSFQGDERVHPDLGGALGDFSSYGWGGM"
BASE COUNT         2431 a         5843 c         5698 g         2116 t
ORIGIN      
        1 atctggatgc ccttcgcgcc catccgcccg atcgcacggt cgatctcctc gagcgccgcg
       61 ggcacgttgt tcatcggcag cgacgcgacc cacgcgggga actgcgcggg gtatttcgcg
      121 cagatctcgg ccatgccgtc gttcgcgagc cgcgcgagcg cgggcgactc ctcgggcccg
      181 gcgaggtact cgatcgcggg cagcgacagc gtcagcacct gcttgtagcc gcggaagcgc
      241 tgcatcatct tgaggcgcgc gtcgaggtcc cacagcaccg ggatgtgcag ccagcgcttg
      301 atgttcgcct tgagcgcggg attgccctcg gcgatctgca tcatgcggtc gaagaacgcc
      361 ttgggaaaga tgtgcgggaa gatgtcgatc ttgaggttcg ggttcattct cgtcgtcgtg
      421 ccgttgggat ggacgcgatt gtctcacgcc ccgtcgtcga ggcgcgtcac gaagcgcgcg
      481 agcgcctcga gcacggccgc gggctggtcg cgatgcggcg agtgtccgca gcggtcgagg
      541 cgcagcgcct cgaccgggcc gctcacctgc cgcacgacgg cgtcgatctg cgccatggtt
      601 ccgtactcgt cgtcgaagcc ctggatcgcg agcaccgggc aggcgatccg cggcaggtgc
      661 tcctcgatgt tccaggcgcg aaactcggga tgcagccaga cgtcgttcca gccgcggaac
      721 gcgccgtccg ggtcggcgtg gtggcgcgcg aggcgctcgc gcaggtcggt cgtctcgaac
      781 gcgaccttcg cctgcgcgat gctctcgacg ctcaggtcct cgacgaagac gtgcggcgcc
      841 atgacgacca ggcccttcag cccgcttttt ggctgcggct gcaccgccgc gtagatgatc
      901 gcgatcgagc cgccgtcgct gtggccgacg agcaccggct cgcgcacgcc cagatcgtcg
      961 agcagccgcg gcaggacctc gagcgcctcg tcgtgcatgt agcggaccgg ccgcgctccg
     1021 cgcagcgggt cggaccggcc gtagccgcgc cgcgaataga cgacggccgc gcagcccgtc
     1081 gcctcggccg ccttgcgagg gaagtcgcgc cacatcgaga tcgagccgag gccctcgtgc
     1141 aggaacacga gcgtcggcgc gtcggggctc gcgcccgcga tccgctggac ttcgagccgc
     1201 gcgccctgaa cggtgacgaa ggatagatcg gaaagctcgg acaagcgggc ccggggtcgt
     1261 tcgctctggg agggagcggc gattctactg cagcttgagc ggcttccagt acacggtcag
     1321 ctcgagatag ccgagaccga gcagcttgcc gtcggcggtc gcgcgcaccg cgccttccca
     1381 gtacacggtg ccggtgctcg cgcgcgcgtc gagctcctgg tcgtccatga gcggctggag
     1441 ctcgatccgg acgtcgcccg ctcgcacgcg catcgccacc gggtagtcgg cgcgggtgcg
     1501 cggcgagcgc cagcgcttga gcggcgtgaa gtcgacctcc tcgggcgcga gcacgcgcgc
     1561 gcggcggtcc ggaccgcgca cggtgccgcc cgcccagagc ttggcgccgt tcttgtcgcg
     1621 gatgcggaac gccatcagcg cgccgccgcc gtcgaggttg acgctgaccc agtcccagcc
     1681 cgccgccccg ggcgcgagat actcgctcga ccactcgtgg tcgagccacg cctcgccgcg
     1741 cacgcccatc gcgcgcccgc cgaccgtcac cgtgccgacc acctccaggt ggggccggct
     1801 gtagtagtag ctcgcctgct cggaccgcgg tcccttgcgg ctcacgccgc gatcgccgtt
     1861 gagcatcagc ggctcgcgcg cctcgaagct gagcgtgtag cggaactcgc gcgccgggat
     1921 gtcggcggcg tagccgttgg ccgtctgctt gagggaccag tcgccgatgg cgacgtcggt
     1981 gccgccttca cgcgcgtccg cgaggccgag accggcgcgc gcggcgcgct ggtcgtggag
     2041 cagccgcccg ttcgcgggat cggcgagcgc ggcgtgcgcg aacagcagct ggcgcgccgc
     2101 gagcgctccc ggcacctcct cggcgacgcc cgggcggttc cggaagaacg tgatctgcat
     2161 cccgcgctcg ctgccgtcgg aggcgcgcag ccatccggtc acgtaccacc attcggtgcg
     2221 gaagcgcgga tggctgccgt gatcgtgcgg gaagcgcagc gcgatgcccg cgctcaccgc
     2281 gggatactgt acctgcgccc cggcggtgcc gacgagccac agcgccgcga cggcgagcgc
     2341 cgcgaactgc gcgcgcgtcc ccgggcgtct cacgggtccg ggctttctcg cgggcgcatc
     2401 agcaccggac gcagcgcggc gagatccagg ttggacctgc gcgggccggc gtccttggtg
     2461 atcgcgatca tgttgtgatg gaacgcgacc gcccctatcg tctcctgcag ccccgcctcg
     2521 ggcgcgggcg gctcgaggaa ttccggcagg ttcacgctgt cggcgagcgt cttgaagaag
     2581 ctcatgcacg agccgccgta ctcgcgcgtg ccgagcacgc cgccgccgaa gtatggccag
     2641 tacgaggtct gcacgtcctc gacgacgtag acgccgccgt ccttcacgaa cggccagagg
     2701 atgcggaagg actcgatctg gtgctcgctg cggtgactgc cgtcgtcgac gacgaagtcg
     2761 aagggcccga tgtcgcgcgc cagagccgtc agccgctcgc ggtcggtctg cgagcactga
     2821 aacaccttga tgcggccgct cgagagcgca gtcttgtcga cgatatcgat gccgtagatc
     2881 ctccccttgc ggaaatacgc cgcccacatg aggagggact cgccgccagg gcggccgccg
     2941 aagtcgccga cgccgatctc gagcagcgag acgctccggc cgcgacgcgc cgcggtgagc
     3001 gcggcgtaca cgggcgtgta catgtgcagg ccgtacttgt cggtgccggc gagggcggcg
     3061 agcaggtcca tgtccgcgcc cagcattccc gcgagcggca gccatcgctg cagcgatcgg
     3121 tgcagcgcgc gggcgccggc gtcgcgctgg aacccgggca ggtcggggat cgcgccgcgc
     3181 cgcgcccgca gcccgaatat gaggtcggca agggccattg ctgcctgttc tcctccttac
     3241 cagtcttcct tgaccgcgag caccgcttcg cggctcatcg cgcggcggcc gctcgccagc
     3301 gcggtcacgg tcgagagtcc gagcatcgcg agcgcgaaca cggcgagcga cgtccacggc
     3361 acatgcaggt ccatgctcca gtggaaagac tggcggttga cgacgtcgat cagcaccagg
     3421 ctcacgagcc agcccagcgc gagcccggcc gcgagcccga gcgccgacac gagcacccct
     3481 tcgaggccca gcatcgcgcc gatcggcgcg cgcgtcatgc cgacgtggcg cagcattccg
     3541 aactcgcgcc ggcgcgcgag caccagcgcg ccgaacgagg acgcgaggcc gagcagcccg
     3601 aggatcaccg ccgccgcctc gagcgcgtag gtcaccgcga aggtgcggtc gaagatgcgc
     3661 agcgaaacgt cgcgcagctc gcccgggcgt gcgagctcga gattggcgcc gccggggatg
     3721 ccggacagcg cgcgctcgac cgcgtcgacg ctcgctcccg gcgccagcca cagcgccgcc
     3781 tcgttcgcgc tcgcgtcgcg cgtgagcgcg atgtagacgt cgcgctcgat cagcaccgcg
     3841 ccctgctggc gcgcgtagtc ccgccagacg cccgcgacga cgaacgcggg caggccgtgc
     3901 gggccgacat ggcccggctt cggcgccttg tcggcgagcg gaatcggcag ctcgatcacc
     3961 tgccccgcgc gccagccgta caggtcggcc gcgatctcgc tcggccaggc ggcgggcggg
     4021 ccgccgggga cccgatcgcg cgccgggccg acgagcggcg gcaggcggcc ctcgcgctcg
     4081 acgccgcgcg cgatcagcac gatcgccggg cgcgccggcg cgaggacgag acgccgcgtg
     4141 cgctggaatt ccgcgtgccg cacgccggcg accgcggcga gcgcgcgctg gtcggccggc
     4201 gcgaggaagg ccgagtctcc gccgaggccg gcgcgcacgt acaggtccgc gggcagcacg
     4261 cgctcgagcc agtcgtcgag cgactggcgg aacgacgcga tcatgatcgc catcgacacc
     4321 atgaggctca cgccggcgac gatcgcggcg aggctcacgc ccaccggacc gggcgcgccg
     4381 cgcagctgcg cgagcgcgag gcccgccgag ggcagccgga agcccggcag cttgcggaag
     4441 aacaccaccg cgatccgcgg catgagggcg atggtgccga ccagcagcag ggcgatcgaa
     4501 acgtagcccg cgatcggcag cgcgcgtatc gggcccgctt gcgtgagcgc cgcacccagc
     4561 gccagcatcg cgacgcccgg ccacgcagac cgcagcggcg cgaacgcgcg ctgctcgtcg
     4621 ccggccttca gcgcggcggc cggctgcgcg cgcgacgcct cgagcgccga ggcgaggctg
     4681 ccgaggagcg cggtcgccgt cccgaggagg aagaagccgg ccatcgccgc ggcgtcgaag
     4741 gaaacggcgg gtctcagccc gcggaagtat ccggcgccca gctcggcgcc gaagtagcgc
     4801 agcacgcctt cggcgccgat ctgcccgagc accaggcccg cgagcgcgcc gagcgcgccg
     4861 atgaccgccg cttccccgag caggagcgcg acgatcccgg cgcgcgtcac gccgagcacg
     4921 cgtagcagcg cgagctgcgc gcgccggcgc acgaccgcga gcgcctgcgt cgagaacacg
     4981 agcagcccgc cggtgaagag cgcgaccagc gacaggacgt tgaggttcac ccggtacgag
     5041 cgcgacaggc tcgcgccgcg ctcggcatcg gcgtcggccg gttccaccac gacgcccggc
     5101 ggcacctcgg cgcgcagccg ggcggcgaac gcctccgccg gcacgcccgc gcgcagccgc
     5161 aggtcgacgc ggtcgaggcg cccggcgcgt ccgaacgccg cctgcgccgc cgcgacgtcc
     5221 atcatggcga cgcgcccgcg gatcgcgccc gagggcagca cgcccgcgac acggaaggtc
     5281 agtgtgccga gcccgacctg gagcgcgagc gcgtcgcccg gcgccaggcc cagcgcgtcg
     5341 gccgccgacg ccgacaggaa cacgcggtcg ggacgcagcc agtcgaggcg atcgccgacc
     5401 tcgccgccga acgcaagggg ctggatcagg gcggcgcgga acacgtcgac gccctgcacg
     5461 cgcagcgcgt cgcgccgtcc cggcactttc gcgtcgacct cgaccaccgg gctcgccacc
     5521 gcgacctcgg gcaggcgcgc gaggcgcgcg tacagcccct cgtcgaagcc gccgcccgcg
     5581 cccgccgcgc cgccgcggac gtggacatcg gcgtcgccgg cgagcgtgtg cacggcctgc
     5641 gtgaactcgg agatcgccgc gtcgttcacg agctgcaccg agtagccgag cgcgacgccg
     5701 agcgcgatcg cgccgatgga aagcgcgagc cgccccttgt gctcgagcat gggcgcgagc
     5761 agcagctcgc ggaacgccgc acggatcatg cgcgaatggt agcaaaccgg tgcccgccca
     5821 cgcgctgcga tagacttgtc gccgcgagga gacgtccatg ccgccgttgc agctcgagcc
     5881 caggctcgcc gggatcctgt ccgccgaata cccgcgcttc tccgacgccg agatggcgcg
     5941 ccggcgcgaa gcgatcgagc gcgtcatgcg cgcggccgaa gtcgatcacc tgctcgtgtg
     6001 cggcgagcag cgcgccggca ccggcgtcgg ctggctgacc tcgtggtcgg cgaccaccga
     6061 ggcgatcgtg atcgtcgcgc cgcccgagcg ccaggtgatg gtcatggagt ggtacaacca
     6121 ctggccgctc gcgaagctgc tcgcgcgcgg caccgaggtg cgctggggcg agcaccgcgg
     6181 catcgacaag gtgatcgccg acctgaagac gcgcaaggtg aggcgcctcg gcttcatggg
     6241 cttcctcggc ctgtcgaagc tgcgcaagct cgaggccgtg ttcggaccgc tcgtcgacct
     6301 gaacgccgag tacgtgaagc tgcggctggt gaagtcgaag gaggagatcg actggatgcg
     6361 catcggctgc gcgctcaccg atctcgcgat cgacgcgctg cggcgcgagg cgcgcgccgg
     6421 catgagcgag cgcgagctgg gcgcgatctg cgaggcggcc taccacccgc acggcggatg
     6481 gaccttcatc cactatttca tggcgacacc gatggacgac ccgcagtact gcgtgccgcg
     6541 ccagatcgcc tcgagccgga ggatccgcaa aggcgacgcg atcgcgaccg agatcaccgc
     6601 gcacttcttc gactatccgg gccaggtgct gcgcagcttc acggtcgacg ccgagccgac
     6661 gccgctcttc cgccgcctgc acgagacggc ggaagccgcg ttcgacgccg tcaccggcgt
     6721 catccggcac ggcaccacga tgcagcagat catcgacgcc gccggggtga tcgaggacgc
     6781 gggcttcacc gtctacgacg atctcatgca cggcttcggc ggcggctatt ttccgccggt
     6841 gctcggcagc cgcagccgac ccgcggggcc gctgccgcac ctgacgctgc agtcaggaat
     6901 gacctgcgtg gtccagccca acgtgatcac ccccgatcag cgcgccgggg tgcaggtcgg
     6961 cgagctgatc ctcgtgaccg ataccggctt cgagcgcatg caccgcgccg agcgcggctt
     7021 tttccgcatt tcgggcgcgt agcacgacgg cataaaatca cgcgccggcg cgcacgggcg
     7081 gaacggcgac ggccgaggac ggtccgacgt aggttgccgg agtcgccatg cgcatctacc
     7141 tgctcgttcc gctccacacc agcgatccga tctggatgtt cagcctccag cgcgaggccg
     7201 tccaggtcat cgccccgagc gagaccgagg cgcggctgcg cgcgagcctg cgctacggcc
     7261 ggcgccgcac gcgcgagggc gccgtgtcgc cgcgcgatcc gtggctcgac gcgaaatggg
     7321 tgtacgcgca cgtcatcgag cacacggacg ccgcgatgcc ggtgctcgcc tgggagccgg
     7381 tcccgatcca gccaacggcg tcccgggccg accctctccc ctcgctgctg cagcgctccg
     7441 acgagccgcg cgacgaccag ccccggagct agccggggtg ctcacccgac gaggcgcgtc
     7501 gcgttcttgc gcagcgactc gggaatctcc aggccgagcg ccttggcggt cttcaggttg
     7561 atgaccagct cgaacctgga cggctgcgcg accggcagct cgctcggctt ggcgccgcgc
     7621 aggatgcggt cgacgtagac ggccgcgcgc cggtacagct ggttgaaatc ctgcccgtag
     7681 ctcatcagcc ctcccgccgt gacgtgctcg cgcacgccga acaccgacgg caggcggttg
     7741 ccgagcgccg ccaccgcgag ctcgcggcgg tactggacgt agatcgggtc gaacgggacg
     7801 atgactccct gcgcgccctc ggccgacatc accctgaacc cgctctcgat ctcgcccgcg
     7861 gtgcgcgcct ccacgcgctg caccctcacg ttcgcggcgc gcgccacttc caggatgctc
     7921 ttcagccgcg gcgagtagaa ctcgttgtcc ggattgagca gcaccgcgac gcgctcgagc
     7981 ttcggcgcga acgccatcag cagctcgagg tagcgcgggc tgacctccac cggcgcgcgc
     8041 gcgacgcccg tcacgttgcg ctcgggccgc gcgagattgg tgatgaaccc gctggtcgcg
     8101 ggatcggccg ccaccgccat gacgatgggg atggtggtgg tctcgcgctg cacggcgcgc
     8161 gtggcgccgg tgcccgaggt gacgatgacg tcgggcgcgc cctgcaccag gcccttcgcg
     8221 agctccggca gccgctccgg cttgccgtcc gcgaagcgcc agtcgaacac tacgttctcg
     8281 tcctcgacgt agccgagctg ggccaggcgc ctgaggaagc tcccgtagta gtcgttcgtc
     8341 cacgacgcgg gccgcgagcg cagcgcgaga aagccgatgc gcttgatcct gcgcgcggga
     8401 gcggcgctct gcgcgatcgc cgcgagcggg cccaggaggg cgtggagcgc ggcgacggcg
     8461 agcagggttc tgcgcttgtt catctctctg gaagagaggg cggggcgggc ctgacaattt
     8521 acacccgtcg cagcgaggcg acagcggtct ttacttagtt ttacacgggc cgactaggcc
     8581 ggtcggagcc cgccttcgca gagcacgcag atccggtcgg cggtgcgcgc ggccgccgcc
     8641 gagtgcgtga ccaggatccc ggcgctgccc tcgcccttca cccgctcgcg cagcagggcg
     8701 agcaccgtcg cggcagtctc gggatcgagg ttgccggtcg gctcgtcggc gagcacgagc
     8761 cgcggcccgt ggacgagcgc ccgggcgatg gcgacgcgct gggtctcgcc gcccgagagc
     8821 tcccgcggca ggctctgcgc gcgctcgtcg agccgcaccg cgcgcagcat cgccgcgacg
     8881 cgcccgccgg cctccgcggc cgggacgccg ttcaggagca gcggcagccc gacgttctgc
     8941 gcgaccgtca ggtacggcag gatgtggaac gcctggaaca cgaagcccat cttccggcgc
     9001 cgcagccgcg tgcgcgcgtc gtcgcccagc gcgaggagct cggcgccctc gaacgcgatc
     9061 gtgccttcgt cgggccggtc gaggccggcg atgaggttga gcagggtcga cttgccgacg
     9121 cccgactcgc ccatgaccgc gacgtactcg cccgcggcga gatcgaagtc cacgccgcgc
     9181 agcaccgcgc gcgaagcccc gaaggctttg gacacgccgc gcagcgacag cagcggcgcc
     9241 tcagccacga acgggctcct cggtgccgag atacgcctcg cgcaccgcgc ggctcgcgcg
     9301 gatcgtctcc ggcggcccgg actcgagcac cgcgccgttc accatcaccg tgagccggcg
     9361 cgcgaccgcg aacaccgcgt ccatgtcgtg ctcgaccagg agaacggcgt ggtcgggcgc
     9421 gagccgccgc agcagctcga cgatgcgcgc cgactcctcg gctcccatcc cggcgagcgg
     9481 ctcgtcgagc aggagcacgc gcggcgaggt cgcgagcacc atcgcgatct cgacctggcg
     9541 ctgctcgccg tgcgacagcg cggcgaccgg cgtctcggcg cgcgcctcca ggcccgcggc
     9601 cgcgagcgcg cgctcggccg cctcgttgac ggcgcggttc gcgagcgccg gccgcaggaa
     9661 gcgcatcgag ctggcgaggc gcgcctgcgc cgcgatccgg cagttctcga ccgccgtgaa
     9721 cggcaggaac acgttcgtct tctggtagct gcgtccgacc ccgagcctcg acatgcggtg
     9781 tggcggcagg cccgtcacgt cgtccccgtc gagctcgacc gcgccggcgc tcgggcgcag
     9841 ctctcccgag agcacgttga tcagcgtcga cttccccgcg ccgttcggcc cgatgatcgc
     9901 gtgcacctcg cccacgcggc actcgatcga gacttcgcgc acggcggtga gcccgccgaa
     9961 gtggcgcgtc agcgcgcgcg tggcgagcgc gggccgctca gccattgttc cgctccgcgg
    10021 gacggcgcag cgagcgcgcc agcccgacca ggccgtgcgg caggacgagc gtcgcgacca
    10081 cgatgaacag gcccatgaac atctgccagt gcttgccgag gttcacgccg cccgcctcgg
    10141 gcaggccctg gagcccgagc tcgagcagca tccacgcggc ggcgcccagg accggcccga
    10201 gcagcgtccc catgccgccg aggatcacca tcatcatcac cgcggccgaa aggtgccatc
    10261 ccatcgtctc gggattgacg acgccgaact gcaccgcggc cagatagccc gcgatgcccg
    10321 cgatcgcgcc cgagatgacg aagcacgcca gcttgtaccg gaacgtcggg tagccgagcg
    10381 agatcatgcg ctgctcgttg acgcggatgc ccgagacgac gcgcccgaac ggcgagcgca
    10441 ggaccgcgcg cagcagcagg tacacggcgg cgaggaacgc cagcgcgagc cagtacacgt
    10501 ggcggtagtc gtcgaggctg aacggccgcc aacccgcgag atcggcgacc gggcggacgt
    10561 cgatgtagat gccgtcgttg ccgccggcga tcttggtgtc gtagaagagg aagaacagca
    10621 tctgcgcgaa ggcgagcgtc accatgatga agtagatgcc gcgcgtgcgc acgacgaaga
    10681 aaccgatcgc gagcgcgagc agcgcgctcg ccgccaccgc gagcggcagc gaggtccaga
    10741 agctcgccgc ctggtattgg ggcgtggtca gcgcgagcgc gtagccggcc gctccgtaga
    10801 aggccgcgtg gccgaagctc accagcccgg cgtagccgag cagcaggtcg aggctcatgg
    10861 cgaagatcgc catgatcatg atcttggcga agaactgcac gtagaacgtc tcggcggcga
    10921 gcgggacgag ggcgagcgcg gcgacgcaga gagcgagagc gagcgccgat cgactcatcc
    10981 ccatcacgtc cgcctcccga aaattccctc cgggcgccac agcagcacca ccgccatcag
    11041 caggtagacg gtcatgctcg agagctcggg gagcacgcgc acgcccgcga tctcgaggtg
    11101 catcaccttg ccgaaggtgt cgacgaggcc gatcagcagc gacgctagca tcgcgccttt
    11161 caccgagccc atgccgccga tcacgatgat gacgaaagag agcacgagga ccgggttgcc
    11221 catgccgggc gcgatcgccg acacgggcgc cgagaccatc cccgcgaacg cggcgagcgt
    11281 gatccccccg gcgaacacga cgcggtagag cgcgtgcacg tcgatgccga gcgattgcac
    11341 catctgccgg tccacggcgc ccgcgcggat ggtcatcccg aggcgcgtcc tctggatgac
    11401 ccacacgatg aggagcgcca cggcgaggca cacgccggag atgaacaggc ggtaaaccgg
    11461 atacgacagc gtatcgctga gcgggatcga cgcatccagc gaggcgggca ccggcacgcc
    11521 gcgcacgtcg tcgccccaca gcaggctgcg catctcctcg aacacgagaa tcaggccgta
    11581 ggtgagcagc acctggtaga ggtgatcgcg gccgtagagc cgacggatga cgagccattc
    11641 gagcagcacc ccgaccgcgg cggtgatgac gatcgccgcc gggaccgcga tccagaagct
    11701 tccgagccgc gccgtcagcg accaggcgag atacgcgccg atcatgtaga agctgccgtg
    11761 cgcgacgttg atgatgccca tgatgccgaa caccagcgtc agcccgctcg ccacgaggaa
    11821 cagcagcagc ccgtactgca cgccgttcag cagctggatc aggaaggtgt ggaggtccat
    11881 gggcgcaaaa acgaaggccg gctcgcgccg gcctccccgg ggaacgtcac gcctggcggc
    11941 tcacgacatc ttgcacgccg cgatcgaggc ggggtcgtcg tcgagcgctt tcacggcgac
    12001 gccgacgtac ttgttctcct tgcccgacac gcggcgcagg tagatgtcct gcaccgggtt
    12061 gtgcgctttg gacaggtgcc acttcccgcg cgggctgtcg acggtcacct tctccatcgc
    12121 cgcgacgacc gctttcttgt cctcgatcct gccgccgacc gacttgagcg ccgcggcgag
    12181 cagcagcccg gagtcgtagc cctgcacggc gtagacgtcg ggctgcatct tgtgcgcctt
    12241 cgcgtaggcc gcgcggaaat cgttgtcgcg cttcgtgttc aggctgtcgg cgtagtgcag
    12301 gcaggtttcg atcccttggg ccgcctcgcc cgccgcatcg agcacgccgt cggtgaggaa
    12361 gccagggccg acgagcggaa tcttgtcctt caggccggcc tgcgcgtagt cgcgcaggaa
    12421 cttggccgcg ccgccgccgg cgaagaacga gtacaccgcg tcgggcctgg ccgccgcgat
    12481 ctccgtgagc agcgcctgga actcgacgtt cgggaacggc agccacagct ccttggcgat
    12541 cgtgccgccg cctttcgcgt agccgtcctt gaagcccttc acgaactcct cgcccgcggc
    12601 gtagcgccag gtgagcgtga cgacgttgcg gatctgcttg cgctgcgcga gcacggcgcc
    12661 catcgcgtag cccgactgcc agttcgagaa cgaggtgcgc acgatgttcg gcgcgcacaa
    12721 ggggccggtc gccgcgccga cgccggcgtt cgggatgatg tgcagcgtgc ccgactcctt
    12781 ggtcacgcgg atcatccccg cggcgacgcc cgagtgcacg gtgccgagga cgacgtcgac
    12841 cttgtcgcgc gtcacgaggc gattgacgtt gtcggtggcc ttcgcgggct ccgactcgtc
    12901 gtcgaccttg tagaactcga cctcgcggcc gccgagcctg ccgcccgact cgccgagcgc
    12961 cattcggaag ccgttctcga tcgccacgcc gagcggcgcg tacgtgccgg tgtacgggag
    13021 catcagcccg accttgatct tcgcgccctg tcccagcacc aggcccggaa acgcaagccc
    13081 cgctccgagc acgccggctc ccttgatgaa gccgcgccga tcgaacatct tgttcttcat
    13141 gtcccgctcc tccttggaga atggcggcct attctacgcc ggccccccca gcgccgcctc
    13201 gatcgtgagc ccgatcacga gcacgcgctc gtcctcgccg gggaagccgg ccacggtcag
    13261 cccgacgggc gcctcgccgg ggcgctggat gggcaggctc gcggcgcacg tgtcgaggaa
    13321 gttgaacggg ctggtgttgc gcagcgtcag cggattcacg gcgaagaagc gctccatgtc
    13381 gcgctcgagc ggctcgatcg ccggcgcgat cttcggcacc gtcggcagga gcagcgcgtc
    13441 gacgccgtgg cgctcgcgcc ggaagcccga gaccaggcgc gagcgcagct gcagcaggtc
    13501 gacgtagtcc gcggccggaa cgtccttcgc ggcctcgagc cggcgccgca cgcgcggatc
    13561 gtagtcggcg ccgcgctcgg cgagccgctt gcggtgcagc gcgtaggcct cgggcgcgag
    13621 gatgccgccg cggcggttcg cggcggcgat ctcgtccagc tccggcaggc gcatgtcgac
    13681 cagccgcgcc ccccgcgacg cgagccgcgc gagcgcggcc tcgaacgcgc gcgccacttc
    13741 gggatcgaga tcgtcgagaa ccacgttctt cggcacgccg agccgcaggc cggcgggcgg
    13801 cgccgcctcg agcgcgcgca ccggctccgc ggcgagcacc tggaacgcgg tcgcgcagca
    13861 ggcgatcgac ggcgcgagag ggccgatcga gtcgagcgag cccgagagcg gaaagcaccc
    13921 ggcgagcggc acgcgccgct gcgtcggctt gaaccccgcg acgccgcaca gcgcggcggg
    13981 gttgcgcacc gagccgccgg tatcggtgcc gaggccgacc accgccatgc cgtcggcgac
    14041 cgacacggcg gcgccggaag aggatccgcc ggggatccgg cgagtcgcgc ggtcccacgg
    14101 gttcgccggc gtgccgtagt gcggattgat gccgatgccc gagtaggcga actccgtcat
    14161 gttggtgcgg ccgatcacga ccgcgccggc ggcgcgcagg cgcgcgaccg cgacggcatc
    14221 ggcgctcgca gcggccgcgt cgcgcagcac cttcgagccc gcggtggtga cgtcgccggc
    14281 gacgtcgaaa agatccttca ccgagaccgg gatcccggcg agcggcgacg gcacggcgcc
    14341 ggccttgcga ctgcggtcgc aggcgtccgc gtcggcgcgc gctctctccg cgtagacgcg
    14401 gatgaacgtg cgcgcgccct cgcccgccgg atcggcgatg cgcgcgagcg cgcgctcgat
    14461 cagctcgcgg ctcgacgtgc gtccttcggc caggtcgcgc gcaagcgacg cgacggtcgg
    14521 caggctcacc gcttcgcgcc ctcgtcgtag taatgcagct cgagcagcag gcagcccgag
    14581 tccgagcgga acgggccgtg cggcgtcccc ggcgggcggc aggcataagt gttgggcccg
    14641 tacttcgtgc cgcccttgcc gttccggtcg cagccgacga ccaggtcgcc cgacacgagg
    14701 tacacctcct cccagtagtc gtgcacgaac ggctcggtcg tgtagacgcc cggcgcgaag
    14761 cgcaggtgcc gcgtgcggaa gccgcgcttc gctcgctcgt cgaggaagcc ggcgaggatc
    14821 tgctgctgga ttcccgacgg atagccctcg ggcgtctgcc agcccttccc gagatcgacg
    14881 gtgtgaaact ccttgtgttc tttctgtatc gccatggtcg tcctccggat caatccgcga
    14941 tctcgagctt cgtgaccgcg taggcgtgcg cgagggcgcg ctttttcacc gggtcctcga
    15001 gctcgatctc gaagcgctcg ccgccgccga tctcgccctg caccggcagc gtgccgcaga
    15061 acatcgcggt gcccgcgggc agcgcgcccg gggacgccgg catgcgcgcg atcagctcgc
    15121 gcggcgcgag catccgcgac acgggaccct cctggtagag cagccggcgc cccgcgcgca
    15181 cgacgtgcga gcgcagcacc agccggtccc agtggtcctc gaggtccgcg agccgccaca
    15241 gctccggccc gatcggcttc gggcacatct gcttcgacac cgtgacgccg taggactcga
    15301 ccttgcggtc ggtatgatcg gatccgacgc cgacccagac gccgtcgctt gccgatacga
    15361 gcacgaactc cacctcgccg ctggagtcgc cgccgacgac ctcgatcgaa ggcgccgtgg
    15421 tcagcaggct cgcgccgaca cggtagaagc tcggcaccgc gcgcggcggc ttgaccccga
    15481 tcgcggcgag ctcggcgatg tgatggtcga ccgccgcgcg gtcgcgcccg gtccagccgg
    15541 cgatgacgag gcgctcgacg cgcagcgcgc gctcgcggcc ttcgatccgg aacgcgagct
    15601 cggccacgct acatcccgcc ccagccgtag gaagagaaat cgccgagcgc gccgccgagg
    15661 tcggggtgca cgcgctcgtc gccctggaag gagccgtaat tgccgcggta gaacacgagc
    15721 ggatcggcct cctcgcccga cacgccgaac gccgtcacgt gcccgacgaa gatcacgtgg
    15781 tcgccgccgt cgtgctcgac gcggcgctcg cactcgaacc acgcgagcga gtcggcgagc
    15841 accgcgcagc cgttggcgcc gacgtggtgc acgatgtcgt cccagaccag gctccccggc
    15901 cgcgtgaagc ggttcgagac gagcttctgc ttctggctca ggacgttcac cgtgaacgcc
    15961 ggagcccggg cgaacacctc gtagatgtgc gcccggcgcg cgaggctgaa cagcacgagc
    16021 gccggctcga gcgacaccga tccgaaggag ttgacggtga ccccggtggg ccgcgcgtcg
    16081 gtcgtcag
//
LOCUS       DATDMW010000360        14639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_601750, whole genome shotgun sequence.
ACCESSION   DATDMW010000360 DATDMW010000000
VERSION     DATDMW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14639
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601750"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VI319_15030"
     CDS             complement(<1..362)
                     /locus_tag="VI319_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865207.1"
                     /translation="MKKIHVVLVSVLVGTAGFHPARAQGPFQAPAARPAPNTSGVARV
                     QHPYRITRLAGPGNQFFIQSVNPETISLGQTAPGKIAISATVNVKYKALGVIMHRQDQ
                     PVDIWNNLDHCGVSGNSPT"
     gene            complement(386..946)
                     /locus_tag="VI319_15035"
     CDS             complement(386..946)
                     /locus_tag="VI319_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865208.1"
                     /translation="MSNALAKRVLLVVPGLVAGSILLGLAPVNPARAADLFQPQAPAA
                     QPAPNISGIARGAQRPYRITRRSGTGNKFYIESVTPVSISLGAVAPKTIAVSATVSLR
                     LNGVVFLHDPSVPLDALHAPSCGREGKSQTCTATYETANLRDGEPLRFAMTMAANVQG
                     SVDLVESYLVEIPVHRAKTRMRVVPR"
     gene            1211..1284
                     /locus_tag="VI319_15040"
     tRNA            1211..1284
                     /locus_tag="VI319_15040"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:ccc)
     gene            1413..2318
                     /locus_tag="VI319_15045"
     CDS             1413..2318
                     /locus_tag="VI319_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEY6865209.1"
                     /translation="MPASIDGKLIAAISSRALFDLEESNRVFEEQGVEAYYEYQLQHE
                     NEVLAPGIAFPLVRKLLALNVLPPARPRVEVILLSRNTADTGLRVMHSIEHYGLDMSR
                     AAFTGGESTYPYVPAFDAHLFLSANPDDVRKALDAGHAAATILPSNVGQNVEATEGLR
                     IAFDGDAVLFSDEAERVYKRDGLQAFTRSEVTAAREPLSGGPFKGFLAALHRIQSEFP
                     AELSPIRTALVTARSAPAHERVVRTLRAWDIRIDEALFLGGLDKGPFLKAFGADIFFD
                     DQRGHCDSARQHVATGHVPFGIANQ"
     gene            2315..3355
                     /gene="mutY"
                     /locus_tag="VI319_15050"
     CDS             2315..3355
                     /gene="mutY"
                     /locus_tag="VI319_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY6865210.1"
                     /translation="MKRAGFAAKLLRWQRTHGRRGLPWQSSRDPYRVWLSEIMLQQTQ
                     VATVIPYYQRFLERFPDVASLARASLDEVMRLWSGLGYYSRARSLHAAARAVAAQGGR
                     FPRTRPALEELPGVGRSTAAAIASLAYGAREAILDGNVKRVLARPFAVAGDPGAPTVA
                     RELWALAESLLPERDNARYTQALMDLGATVCRPRDPECGRCPVARSCAARRSDAIELY
                     PGRKARKPLPVKRTAMLLLVHRGRILLEKRPPSGIWGGLWSLPEMRSGLDPVPYCEAR
                     YGARLASVEKLQALTHGFTHFALHIRPVRCQVGSLEPRTAEPGWAWLEPAEAAGGAVP
                     APVRRLLLDARG"
     gene            complement(3362..3952)
                     /locus_tag="VI319_15055"
     CDS             complement(3362..3952)
                     /locus_tag="VI319_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY6865211.1"
                     /translation="MKLPIFPLNTVLFPDGLLPLRVFEARYMDMTRDCLKQNRPFGVC
                     LIREGAEVGAPAVPETVGCLARITDWDMQQQGVLGLKTRGGQRFRIVSSSVGSQGLIS
                     ADAELIAPEQKIRVPREYDGCAKLLRMLVSDQGNAIFAEPHAFDDAAWVGYRLADILP
                     VPLAAKQKLLELTDSLQRLAILQRFLEQRGLAQPKA"
     gene            complement(3988..4602)
                     /locus_tag="VI319_15060"
     CDS             complement(3988..4602)
                     /locus_tag="VI319_15060"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF004685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEY6865212.1"
                     /translation="MDAAKTVVVALTGASGMAYGMRLIESLVRADARVFLLYSQAAHV
                     VAKQELGVVLPPRPAEAERHFGERYGAKEGQIRAFGRDDWFAPVASGSNPADAMVICP
                     CTTGTLAAVAMGLSDNLIERAADVMLKEGRKLVLVPRETPFSVVHLENMLTLARAGAV
                     ILPANPGFYHRPTRIEELVDFVVARILDQIGVPNRLAGRWGEER"
     gene            complement(4607..5446)
                     /gene="rapZ"
                     /locus_tag="VI319_15065"
     CDS             complement(4607..5446)
                     /gene="rapZ"
                     /locus_tag="VI319_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY6865213.1"
                     /translation="MQLVLISGLSGSGKSIALRVLEDSGYYCVDNLPAQLLEQTVDLL
                     RRAGHANVAVSIDARTGGALDLLPGYLAALRRQSVDVRVLFLDAKTETLIKRFSETRR
                     RHPLAKGDLTLEEALARERELLSVIAETGQRIDTSGMVPNTLRKWIKELLAIPSETTT
                     LLFESFAYKQGIPLDADLVFDVRCVPNPFYEPRLQPQTGLDADVAKFLESEPQVGALA
                     DDIGRFVESWLPAFHREHRNSLTIAIGCTGGRHRSPYFAERLGRRFRDRASVLIRHRD
                     LAG"
     gene            complement(5487..6440)
                     /gene="hprK"
                     /locus_tag="VI319_15070"
     CDS             complement(5487..6440)
                     /gene="hprK"
                     /locus_tag="VI319_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEY6865214.1"
                     /translation="MFQISVARLYEENREKLQLAWVAGKPGGEAPLQRDSSDAPALVG
                     HLNLIHPNRIQVFGAHEASYLRSLDAPRSQQLLEELFAARPAALLFADGVAPDARLID
                     RAERSGTPVLASSAPGAQVIDRLRRYLGKALADGTQRHGVFMDVLGLGVLITGDSGVG
                     KSELALELVSRGHGLVADDVVDISRIAIGTLEGRCPPMLRDFIEVRGLGVLNIRTIFG
                     ETAVRPKMNLKLIVQLQRPPQAGAEPERLPLHELSEDILGVTVRKVVIPVAAGRNLAV
                     LIEAAVRNYILQLRGIQSAAELIERQNAEMRRQAPPGDTGR"
     gene            complement(6409..6894)
                     /gene="ptsN"
                     /locus_tag="VI319_15075"
     CDS             complement(6409..6894)
                     /gene="ptsN"
                     /locus_tag="VI319_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEY6865215.1"
                     /translation="MNLIAKLLPPSNIVLDLDVTSKKRMFEQAGLLFENNQGVARSLV
                     FDSLFARERLGSTGLGHGVAIPHGRIKGLKEAVGAFVRLAQPVPFDAPDGKPVSLVFV
                     LLVPEHATERHLQILSELAQMFADRELRERFASDDNPGLLHQHITEWNPHVPDQRRAA
                     V"
     gene            complement(7006..7329)
                     /gene="raiA"
                     /locus_tag="VI319_15080"
     CDS             complement(7006..7329)
                     /gene="raiA"
                     /locus_tag="VI319_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY6865216.1"
                     /translation="MNVTVSGHHVAVTAAIRSYVETKLSRISRHFDHVIDVNVILSVD
                     KTGQKAEASCHVRGKEIFVESDDQDLYAAIDLLVDKLDRQIIKYKDRVRSHPHDALKH
                     RDSAG"
     gene            complement(7389..8819)
                     /locus_tag="VI319_15085"
     CDS             complement(7389..8819)
                     /locus_tag="VI319_15085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY6865217.1"
                     /translation="MKQTLQLKLSQHLTLTPQLQQSIRLLQLSTMELNQELEKFLADN
                     PLLEREEPAAETPITRMNGEAAPVQVTADEARSEPAQAESAPSELDWLADAAPSGGAR
                     DDGEEGDYPQLAADSPTLREHLLSQITLTNLESRDRSIVAVLIEALDEDGYLTQPLEE
                     LVELLPAELGIELDELQIGLKHLQNLDPTGIGARDCAECLELQLLALPDSTTGRTLAL
                     SVVRKHLQLLATRDFAKLRKTLQCSEDELRQAQHLIQSLNPRPGAAFSAVETRYVVPD
                     VVVKKVKGVWVASLNPEAVPKLRINRMYAEILQGNRNNSGGQLSQQLQEARWLIKNVQ
                     QRFETILRVSQAIVDRQRHFFEHGEVAMRPLVLREIADTLGLHESTVSRVTTQKFMLT
                     PRGIFELKYFFGSHVSTETGGACSATAIRALIKQLVAAEDAKRPLSDSKIAEILGQQG
                     IVVARRTIAKYRESLHIPPVNLRKSL"
     gene            complement(8946..9668)
                     /gene="lptB"
                     /locus_tag="VI319_15090"
     CDS             complement(8946..9668)
                     /gene="lptB"
                     /locus_tag="VI319_15090"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY6865218.1"
                     /translation="MKQLRADGLKKRYKSRVVVKDVSLEVSSGEVVGLLGPNGAGKTT
                     CFYMIVGLIPVDGGHIHLDAKEISHMAMHSRARLGLSYLPQEASVFRKLTVEENVRAV
                     LELQNLPEDEVQQRLEGLLHDLSIAHLHHSTALSLSGGERRRVEIARALATRPSFILL
                     DEPFAGVDPIAVLDIQKIIGFLKERGIGILITDHNVRETLGICDRAYIINDGSVLAFG
                     KPDEIVYNESVRRVYLGENFRL"
     gene            complement(9697..10311)
                     /gene="lptA"
                     /locus_tag="VI319_15095"
     CDS             complement(9697..10311)
                     /gene="lptA"
                     /locus_tag="VI319_15095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY6865219.1"
                     /translation="MRRNRAVAPWLLVAAVVAALPCAGRAERSDREKPVNIESDRMTA
                     DDAKKTATFEGSVVLTQGTLTIRADRIVVRQDESGFQYGVAYGTPAKFRQKREGADEF
                     VDGEALRLEYDGRAERVELFDDAHLHRDGGDDVRGNYISYDSKTEYFTVNSGKQAAKD
                     GRDSRVRATIMPKKSEPTKNSAPPKPPAPLELKPDTSVPKPGQQ"
     gene            complement(10298..10870)
                     /gene="lptC"
                     /locus_tag="VI319_15100"
     CDS             complement(10298..10870)
                     /gene="lptC"
                     /locus_tag="VI319_15100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY6865220.1"
                     /translation="MKIRAANALPLGLMFLLGAMTFLLQYAVQRGGPGDARPARHEPD
                     AVIENFRVDSLGKDGKPEATFSAPKMFHFPDDDSAEVLYPRMVKLADDGGHLVATANR
                     GVLTKDGEEGFLYGNVVLVREGTPGRESLRAETEFLHIVNDQHVLQTDRPVVITEGNS
                     IVSGVGMEMHQDTRELKLFSQVRGTIDAKK"
     gene            complement(10867..11349)
                     /locus_tag="VI319_15105"
     CDS             complement(10867..11349)
                     /locus_tag="VI319_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY6865221.1"
                     /translation="MVFDVDGVLTDGALYLVQGGEMKVFHVRDGHGVKMLHEIGIQTA
                     LLSGRRSALVDARAAELGIRIVEQGCADKAAGFDKLLSRVGVPASAAGYMGDDFPDLP
                     VIERCGFAASVPDAPPAVRRSAHYVTRAAAGRGAAREVCEFILEAQKTLSRAARRTLA
                     "
     gene            complement(11397..12395)
                     /locus_tag="VI319_15110"
     CDS             complement(11397..12395)
                     /locus_tag="VI319_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY6865222.1"
                     /translation="MASSGASTVGAADSALDLARKVLVIEADAVSALASRIDQSFLAA
                     LDLLLSCRGRVVVSGMGKSGHIARKIASTMASTGTPAFFVHPAEASHGDLGMITREDV
                     VVALSYSGESDELLTIIPLIKRQGAKLIAITGNDASALAKEADVCLDARVSQEACPLN
                     LAPTASTTAALALGDALAIALLDARGFGADDFARSHPGGSLGRRLLTHVSDVMRTGDD
                     VPQVAESASLSDALREMSRKRMGMTAVVDRSRRVVGIFTDGDLRRALERSSDLRAVAL
                     PDVMTRNPRTIQAGKLAVEAVEMMERHKINQLLVVDEQGGLAGALNMHDLFRAKVI"
     gene            12467..14434
                     /locus_tag="VI319_15115"
     CDS             12467..14434
                     /locus_tag="VI319_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY6865223.1"
                     /translation="MGSTLELILLLLASAVVAVTAFRWVHLPPLLGYLLVGVVIGPFA
                     FGLIPDSEQLRRLAEFGVVFLMFSIGLEFNLPTLFTMRRAVFGLGLAQVLLTAGTVMV
                     VAPLAGFAWEPALAVGGALAMSSTAIVMRMLSERMQLDTDHGRSIVGVLLFQDLAVVP
                     LLIVIPAIAHGGDVARQLGLALVKAGIVLGVVLFLGHRLMRPWLHVVAGRRSHELFIL
                     NVLLLTLGLAWLTEIAGLSLALGAFLAGMLIAETPYRHQVEEDIKPFREVLLGLFFVT
                     MGMKLDVRIVAHEAPLVLVLVVLPVAFKLALTAALARFFGSGPGSALRIGLAIAHAGE
                     FGLVLIVQATDVGLLGAEFSQLVVAAMLISMLVSPFILQASDRLVMRWASSEWMLKSL
                     ELHRIAVQSLATENHVIVCGYGRTGQRLAHLLEQEDIRYIALDLDPERVRQAGAAGDA
                     VVYGDASHREALVAAGLARAGALVVTFADTARALKVIHHAHDVNPALPVLVRTLDDAD
                     LDRLLAAGAAEVVPETFESSLMLGSHALMLVGVPLTRVVRRIRDVRAHRYDLLRGFYR
                     GESDEASDPDEARSPRLRSVALHPGARGAGRRLGELALDAIGVTVPAIRRRETRMVRP
                     GPETELAEGDVVVLLGGPEQLAAAEARLLGH"
BASE COUNT         2213 a         4876 c         5012 g         2538 t
ORIGIN      
        1 gtcgggctgt tgcctgacac gccgcagtga tccaggttgt tccagatgtc gaccggctgg
       61 tcctgccggt gcatgatcac gcccagcgcc ttgtatttca cgttgacggt cgccgatatc
      121 gcgatcttcc cgggcgcagt ctgcccgagc gaaatcgtct ccgggttaac gctctggatg
      181 aagaactgat tcccgggccc ggcgaggcgg gtgatgcggt acgggtgctg aacgcgcgcg
      241 acgccggaag tgttcggcgc gggccgcgcg gcgggcgcct ggaatgggcc ctgcgcccgg
      301 gccggatgga agccggccgt cccgaccaag actgaaacca ggacaacatg gatcttcttc
      361 attgcatttc tccctttctt gttcactaac gcgggacgac gcgcatccgg gtcttcgccc
      421 tgtggaccgg gatctcgacc aggtagctct cgaccagatc gacgctgccc tgtacgtttg
      481 cggccatcgt catggcgaag cgcagcggct cgccgtcgcg caggttcgcg gtctcgtacg
      541 tcgcggtgca cgtctgcgac ttgccttctc gaccgcaact gggcgcgtgc agcgcgtcca
      601 gcggcacgct ggggtcgtgc aggaacacga caccgttgag ccgcagcgaa accgtcgccg
      661 acacggcaat ggtcttgggc gcgaccgccc cgagggagat cgataccggc gtcacgctct
      721 cgatatagaa cttgtttccg gttccgctcc gtcgcgtgat gcgatacggc cgctgtgcgc
      781 cgcgcgcgat cccggagata ttcggcgccg gctgcgcggc cggcgcctgc ggctggaaca
      841 ggtccgccgc gcgagccgga ttgaccggcg ccaaccctag aagaatcgag cccgcgacga
      901 ggcccggcac gacgagtaac acccgcttcg ccagcgcgtt gctcatgctg ctcctccctt
      961 ggatacagct tgaaacgtcc tgccatgcgg caccgtcgaa gtccgctttc ttgctgcggc
     1021 tcggacatga aaacgtccgg acgttgtgcg cggttgaccg gtatggatgg ttccccccgg
     1081 tgcgagggag ctccgaagcg gcgcccagcg gccgacctcg agcggccgtt aatgtgccac
     1141 gggcctcaac cgtcttgcaa ggggagccgc ggacccgcgt cgccgaggga atgtcttaca
     1201 atgcgccgac gcgggcgtag ttcaatggca gaacgagagc ttcccaagct caaaacgagg
     1261 gttcgattcc cttcgcccgc tccacctgct gcgcaagtct ttgcggcgca tggcgccggg
     1321 aagacgctcg actcgccggg cgagcaccag cgggcgcgcc gcggtcggcg cgaggcggta
     1381 cactgtcggg gaagaaagaa caactccgac cgatgcccgc gtccatcgac ggtaagctca
     1441 tcgctgcgat ctcgtcgcgg gcgctgttcg acctcgagga gagcaaccgc gtattcgagg
     1501 agcagggggt cgaggcgtat tacgagtacc agctgcagca cgagaacgag gtgctcgcgc
     1561 caggtatcgc attcccgctg gtgcgcaagc tactggcctt gaacgtgctg ccgcccgcgc
     1621 gtccacgcgt cgaggtcatc ctgctgtcgc gcaataccgc cgataccggg ctgcgcgtca
     1681 tgcattcgat cgagcactac gggctcgaca tgtcgcgggc cgcgttcacg ggcggcgaga
     1741 gcacctatcc gtacgtgccg gccttcgacg cgcacctgtt cctctcggcc aatcccgacg
     1801 acgtgcgcaa ggcgctcgac gcgggacacg cagcagccac catcctgcct tcgaacgtcg
     1861 gacagaacgt cgaggctacc gagggcctgc gcatcgcttt cgacggcgac gcggtgctgt
     1921 tctccgacga ggccgagcgc gtctacaagc gcgacgggct gcaggcgttc acgcgctccg
     1981 aggtgaccgc ggcgcgcgag ccgctctcgg gcggcccgtt caagggcttc ctcgcggccc
     2041 tgcatcgcat ccagtcggag ttcccggcgg agctctcgcc gatccgcacc gcactcgtca
     2101 ccgcgcgcag cgcgcccgcg catgagcggg tggtgcgtac gctgcgcgcc tgggacatcc
     2161 gcatcgacga ggcgctgttc ctgggcggcc tggacaaggg gccgttcctg aaggcgttcg
     2221 gcgccgacat tttcttcgac gaccagcgcg ggcattgcga ctcggcccgg cagcacgtcg
     2281 cgacgggcca cgtcccgttc ggcatcgcca atcagtgaag cgggctggat tcgcggcgaa
     2341 gctcctccgc tggcagagga cgcacggccg ccgcggcctt ccctggcagt cgagccgcga
     2401 tccgtaccgc gtctggctct ctgaaatcat gctgcagcag acgcaggttg cgacggtgat
     2461 tccctactac cagcgcttcc tcgagcgctt tcccgacgtc gcgagcctcg cccgcgcgtc
     2521 cctggacgag gtgatgcgcc tgtggagcgg actcggctac tactcgcgcg cccgcagtct
     2581 gcacgcggcc gcgcgcgcgg tcgccgcgca gggcggacgc tttccgcgca cgcgcccggc
     2641 gctcgaggaa ttgccgggtg tcgggcgctc caccgccgcg gcgatcgcgt cgctcgccta
     2701 cggggcgcgc gaagcgatcc tcgacggaaa cgtcaagcgc gtgctcgcgc gccccttcgc
     2761 ggtcgccggc gaccccggcg cgccgacggt cgcgcgcgag ctctgggcgc tcgccgagtc
     2821 gctgctcccc gagcgcgaca acgcgcgcta cacgcaggcg ctgatggacc tcggcgcgac
     2881 cgtgtgccgg ccgagagatc cggagtgcgg gcgctgtccg gtggcgcgaa gctgcgccgc
     2941 gaggcgcagt gacgcaatcg agctctaccc cgggcgcaaa gcgcgcaaac cgctaccggt
     3001 aaagcgcacg gcgatgctgc tgctggtgca ccgggggcgg atcctgctcg agaagcgtcc
     3061 gccgagcggg atctggggcg gcctctggag cttgccggag atgcggtccg gcctcgaccc
     3121 ggtgccgtac tgcgaagcgc gctacggcgc ccgcctcgcc tcggtcgaga agctgcaggc
     3181 gctcacccac gggtttacgc acttcgcgct ccacatccgg ccggtgcggt gccaggtcgg
     3241 gagcctcgag ccgcgcaccg cggagccggg atgggcctgg ctggagcccg ccgaagcggc
     3301 cggcggcgcc gtgccggcgc cggtgcggcg cctgcttctc gacgcccgcg gctgagaacg
     3361 ctcaggcctt gggctgcgcg agaccgcgct gctcgaggaa gcgctgaaga atcgcgagcc
     3421 gctgcaggct gtcggtgagc tcgagcagct tctgcttggc ggcgagcggc acgggcagga
     3481 tatcggcgag ccggtagccc acccatgcgg cgtcgtcgaa cgcgtgcggc tcggcgaaga
     3541 tcgcatttcc ctggtccgag accagcatcc gcagcagctt cgcgcagccg tcgtactcgc
     3601 gcgggacgcg gattttctgc tcgggcgcga tcagctcggc atccgccgaa atcagtccct
     3661 ggctgccgac gctgctcgag acgatgcgga agcgctgccc gccacgcgtc ttcaggccga
     3721 gcacgccctg ctgctgcatg tcccagtcgg tgatgcgcgc gaggcagccg accgtctcgg
     3781 gaactgccgg cgcgccgact tcggcgcctt cgcggatcag gcagacgccg aacggccgat
     3841 tctgcttcag gcagtcgcgt gtcatgtcca tgtagcgcgc ctcgaacacg cgcaacggca
     3901 gcaggccgtc cgggaacagc accgtgttca gggggaagat cggaagtttc accgcggaat
     3961 tccgggcccc gtcgccgggg ccgcgtccta acgttcttcg ccccagcgcc cggcaagccg
     4021 gttcggcacg ccgatctggt cgaggatgcg cgcgacgacg aagtcgacga gctcctcgat
     4081 gcgcgtcgga cggtggtaga agccgggatt ggccggcagg atcaccgcgc ccgcgcgcgc
     4141 gagcgtcagc atgttctcga ggtgcacgac cgagaacggc gtctcgcgcg ggacgaggac
     4201 gagcttgcgt ccttccttca gcatgacgtc ggccgcgcgc tcgatgagat tgtccgagag
     4261 ccccatggcg accgccgcca gcgttccggt cgtgcacggg cagatgacca tcgcgtcggc
     4321 gggattcgag cccgacgcga ccggcgcgaa ccagtcgtcg cggccgaatg cgcggatctg
     4381 gccttctttc gcgccgtagc gctcgccgaa atgacgctcg gcctcggcgg ggcgcggcgg
     4441 cagcacgacg ccgagctcct gcttcgcgac cacgtgcgcc gcctgcgagt agaggaggaa
     4501 cacgcgcgca tcggcgcgca cgagcgactc gatgaggcgc atgccgtacg ccatcccgga
     4561 agctccggtg agcgcgacga cgacggtctt ggcggcgtcc acggcttcag cccgcgagat
     4621 cgcgatggcg gatcagcacc gaagcgcggt cgcgaaagcg ccgtcccagg cgctccgcga
     4681 agtagggcga gcggtgacgg cccccggtgc agccgatggc gatcgtgagg gagttgcggt
     4741 gctcacggtg aaaagcggga agccatgact cgacgaacct gccgatatcg tcggcgagcg
     4801 cgccgacctg gggctcgctc tcgaggaact tcgcgacgtc ggcgtcgaga cccgtttgcg
     4861 gttgcagacg cggctcgtag aaggggttcg gcacgcagcg cacgtcgaac accaggtcgg
     4921 cgtcgagcgg gatgccctgc ttgtacgcga acgactcgaa caacagcgtc gtcgtctcgc
     4981 tcgggatggc gagcagctcc ttgatccact ttcgcagggt gttgggaacc attccgctcg
     5041 tgtcgatccg ttgtccggtc tcggcgatga ccgacagcag ctcgcgctct cgcgcaaggg
     5101 cctcctcgag cgtgagatcg cccttcgcga gcgggtgccg gcgccgcgtc tcggagaagc
     5161 gcttgatcag ggtctcggtc ttcgcgtcga ggaacagcac gcgcacgtcc acgctctggc
     5221 gccgtagcgc cgcgagatag ccgggcagca ggtcgagcgc gccgccggtg cgcgcgtcga
     5281 tgcttaccgc gacgttggca tgccccgcgc gccgcagaag gtcgacggtc tgctccagca
     5341 gctgtgccgg caggttgtcg acgcagtagt agccgctgtc ctcgagcacc ctgagcgcga
     5401 tgctcttgcc cgagcccgac aagccgctga tcagcaccag ctgcataccg cggaattata
     5461 gccgcagagc cgggctttcc cgcggctcag cgcccggtat ccccgggagg ggcctggcgc
     5521 cgcatctccg cgttctggcg ctcgatgagc tccgcggccg actgtatgcc gcggagctgg
     5581 aggatatagt tgcggaccgc cgcctcgatc agcaccgcca ggttgcgccc ggccgcgacg
     5641 ggaatgacga ccttccggac cgtgacgccc aggatgtctt cggagagttc atgcagcggc
     5701 agccgctcgg gttcggcgcc ggcctggggt ggacgctgga gctgcacgat cagcttgagg
     5761 ttcatcttcg gtctcaccgc agtctcgccg aagatggtgc ggatgttgag cacgcccagc
     5821 ccgcgtacct cgatgaagtc gcgcagcatg ggcggacagc gcccttccag cgtgccgatc
     5881 gcgatgcggg agatatcgac gacgtcgtcc gcgaccaggc cgtgaccgcg gctcaccagc
     5941 tcgagcgcga gctcgctctt gccgacgccc gaatcgccgg tgatcaggac gcccagaccc
     6001 agcacatcca tgaagacacc gtgacgctgc gtgccgtcgg cgagcgcctt gcccaggtaa
     6061 cgccgcaggc gatcgatgac ctgagcgccc ggcgccgacg aggcgagcac gggcgtgccc
     6121 gagcgctcgg cccgatcgat gagccgcgcg tcgggcgcga cgccgtcggc gaacagcagc
     6181 gccgcgggcc gtgccgcgaa cagctcctcg agcagctgct gtgagcgcgg cgcatcgagc
     6241 gagcgcaggt acgacgcctc gtgggcgccg aagacctgaa tgcggttggg gtggatgagg
     6301 ttgaggtggc ctacgagcgc cggggcgtcg ctcgagtccc gctgcagggg cgcttcgccg
     6361 cccggcttgc ccgcgaccca ggcgagctgg agcttctctc ggttctcctc atacagccgc
     6421 gcgacgctga tctggaacat gcgggttcca ctcggtgatg tgctgatgca gcaggcccgg
     6481 attgtcgtcc gaagcgaacc gctcccgcaa ttcccggtcg gcgaacatct gcgcgagctc
     6541 ggagaggatc tgtagatggc gctcggtggc gtgctcgggg accagcagca cgaagacgag
     6601 cgagacgggc ttgccgtcgg gcgcgtcgaa cggcaccggc tgtgcgagcc gcacgaacgc
     6661 gccgactgcc tctttcagcc ccttgatccg tccgtggggg atggcgacgc cgtggccgag
     6721 gcctgtggac ccgagacgct cgcgcgcgaa caggctgtcg aagacgaggc tgcgcgcgac
     6781 tccctggttg ttctcgaaca gcaggcccgc ctgctcgaac atgcgcttct tgctggtgac
     6841 gtcgaggtcg agaacgatgt tggagggagg aaggagtttc gctatgaggt tcattgcgct
     6901 agccgccgtc cggcgcctgt cgccagtttg agtctcgtcc agccgaaatc gacgcatgtt
     6961 acaccagcac cggggccggc cgccagcatc gcgggacccc gaggatcagc cggccgaatc
     7021 cctgtgcttc aaagcgtcat gaggatgcga cctgacacgg tccttgtatt tgatgatctg
     7081 ccgatccagc ttgtcgacca gaaggtcgat cgccgcatac agatcctggt catcgctctc
     7141 gacgaagatc tccttgccgc gcacgtgaca gctggcctcg gccttctggc cggtcttgtc
     7201 tacgctgagt atcacgttga catcgattac gtgatcgaag tgacggctga tgcgcgagag
     7261 cttggtctcg acatagctgc ggatggcggc cgtgactgcg acatggtgac cgcttaccgt
     7321 gacgttcatg acgactcctt ctcgggcgct cgtgctccgt tccggacccg cccagcaagg
     7381 cgcgcggatc agagcgactt gcgcagattg acgggcggaa tgtgcaacga ctcccggtac
     7441 ttcgcaatcg tacgccgggc gaccacgatt ccctgctgtc cgaggatctc cgcgatcttg
     7501 ctgtccgaga gcgggcgctt tgcgtcctcg gcggcgacca gctgcttgat gagtgcacgg
     7561 atggccgtgg cggagcaggc gccgccggtt tcggtcgaga cgtggctgcc gaagaaatac
     7621 ttgagctcga aaatgccgcg cggtgtcagc atgaatttct gggtcgtgac gcgcgacacc
     7681 gtcgactcgt gcagtccgag ggtgtcggcg atctcgcgca gaaccagcgg ccgcatggct
     7741 acctcgccgt gctcgaagaa atgccgttgg cggtccacga tcgcctgcga cacgcgcaag
     7801 atggtctcga aacgctgttg tacgttcttg atcaaccagc gtgcctcctg cagttgttgg
     7861 gacagctggc cgcctgaatt gttccggttg ccctggagga tctcggcgta catccggttg
     7921 atgcgcagct tcggtaccgc ctccggattc aggctggcga cccatacgcc cttgaccttc
     7981 ttcacgacga catcgggaac gacgtagcgc gtctcgacgg cgctgaacgc cgcgccgggc
     8041 cgcgggttta gactctggat gagatgctgc gcctggcgca gctcgtcctc cgagcattgc
     8101 agcgtcttgc gcagcttcgc gaagtcgcgg gtggcgagga gctgcagatg cttgcggacg
     8161 acgctcaggg cgagcgtccg gccggtcgtc gaatccggca gagcgagaag ctgcaactcc
     8221 aggcattcgg cgcaatcgcg cgcgccgatg ccggtcgggt cgaggttctg caggtgcttc
     8281 aggccgatct ggagctcgtc gagctcgatc ccgagctcgg cgggcagcag ctcgaccagc
     8341 tcctcgagcg gctgcgtcag ataaccgtcc tcgtcgagcg cctcgatgag gaccgccacg
     8401 atgctgcgat cgcgggactc gaggttggtg agcgtgatct gcgacagcag gtgctcgcgc
     8461 agcgtcggcg agtcggccgc gagctgcggg taatcgcctt cttcaccgtc gtcgcgcgct
     8521 ccgcccgaag gcgccgcgtc ggcgagccag tcgagctcgg aaggagcgct ttccgcctgc
     8581 gcgggctcgc tgcgagcctc atcggcggtg acctgcaccg gcgccgcctc tccgttcatg
     8641 cgcgtgatcg gcgtctccgc cgcgggctct tcgcgctcga gcaatggatt gtcggcgaga
     8701 aatttctcca gctcctggtt cagctccatc gtcgacagct gcagcagccg tatcgactgc
     8761 tgcagctgcg gagtgagcgt gagatgctgc gagagcttga gctggagtgt ctgcttcatt
     8821 gcaagctgtc gcggacggga tggtgcttcc ggagttgttt ttcgaggctc atggaagacc
     8881 agagtatgcg ctgcgagtgc ccgttgtcgc ggaacttttc cagcagaagg gcacgcaggc
     8941 gctctctaga ggcggaagtt ctcgcccaga tagacgcgtc taacgctctc attatagaca
     9001 atttcgtcgg gtttgccgaa tgccagaacg ctgccatcgt tgatgatgta ggcgcgatcg
     9061 caaataccga gcgtttctcg cacgttatgg tctgtgatca aaattccgat cccgcgttcc
     9121 ttcagaaatc cgatgatctt ctgaatgtcg agcacggcga ttgggtcgac tcccgcgaac
     9181 ggctcatcga ggagaatgaa gctcggccgt gtcgcgagcg cgcgcgcgat ctcgacgcgg
     9241 cggcgttcac cgccggacag cgacagggcc gtcgaatggt gcagatgggc gatgctgaga
     9301 tcgtgcagca acccctcgag acgctgctgc acctcgtcct cgggcaggtt ctgcagctcg
     9361 agcacggcgc ggacgttctc ctcgacggtg agctttcgga agaccgaggc ttcctgaggc
     9421 aggtacgaca gcccgagacg cgcgcggctg tgcatcgcca tgtggctgat ctcttttgcg
     9481 tcgagatgga tgtggccacc gtcgacggga atcagcccaa cgatcatgta gaagcaggtg
     9541 gtcttgcccg cgccgttggg accgagcagc ccgacgactt ccccgctgga gacttccagc
     9601 gagacgtcct tgaccaccac gcgggatttg tagcgcttct tgagtccgtc cgcccgcagc
     9661 tgtttcatga gcttcgtccg tcgcgcggcg caacgatcat tgctgtccgg gcttgggaac
     9721 cgaggtatca ggcttcagtt ccagcggcgc aggcggtttc ggtggcgcgg agttcttggt
     9781 gggctccgat ttcttcggca tgatcgtggc ccgcacgcgg ctgtcgcggc cgtccttggc
     9841 tgcctgcttg ccggagttca ccgtgaagta ctccgtcttg gagtcgtagg aaatgtagtt
     9901 tccgcgcacg tcgtcgccgc cgtcgcggtg cagatgcgcg tcgtcgaaga gctccacgcg
     9961 ctcggcgcgc ccatcgtact cgagccgcaa cgcctcgccg tcgacgaact cgtcggcgcc
    10021 ctcgcgcttc tggcggaact tggcgggagt gccgtacgcg acgccgtact gaaagcctga
    10081 ctcgtcctgg cgcacgacga tgcggtcggc gcggatcgtg agcgtgccct gcgtgagcac
    10141 cacgctgcct tcgaacgtcg cggtcttctt ggcgtcgtcg gccgtcatcc ggtccgactc
    10201 gatgttgacg ggcttttccc ggtcggagcg ctcggcgcgt ccggcgcacg gcagcgccgc
    10261 gacgaccgcc gcgaccaaga gccatggcgc gacggcccta tttcttcgca tcgatcgtcc
    10321 cgcggacctg ggagaaaagc ttcagctcgc gcgtatcctg atgcatctcc atgccgacgc
    10381 cgctcacgat ggagttgccc tcggtgatga ccacaggccg gtcggtctga agcacatgct
    10441 ggtcgttcac gatgtgcagg aactcggtct cggcgcgcag cgactcccgc ccgggtgtcc
    10501 cttcgcgcac cagcacgacg ttgccgtaga ggaagccttc ctcgccgtcc ttggtgagca
    10561 ctccgcggtt cgccgtggcg acgagatggc caccgtcgtc ggcgagcttc accatgcgcg
    10621 gatacagcac ctcggccgag tcgtcgtcgg ggaaatggaa catcttgggc gcggagaagg
    10681 tcgcctccgg cttgccgtcc ttgcccaggc tgtcgacgcg gaaattctcg atcaccgcgt
    10741 cgggctcgtg ccgcgccggg cgcgcgtctc cggggccgcc ccgctgcacg gcgtactgca
    10801 acagaaacgt catggccccg aggaggaaca tcagccccag cggcagggca ttggcggcgc
    10861 ggatcttcac gcgagcgtcc tccgggcggc gcgcgaaaga gtcttttgcg cctcgaggat
    10921 gaactcgcag acctcgcgcg ccgcgccccg accggcggca gcgcgggtca cgtagtgggc
    10981 gctacgccgg actgccggcg gggcgtccgg tacgcttgcc gcgaatccgc atcgctcgat
    11041 gacgggaaga tccgggaagt cgtcgcccat gtagccggca gcgctggccg ggactccgac
    11101 gcgcgaaagc agtttgtcga aaccggcagc cttgtcggcg cagccctgct ccacgatcct
    11161 gattccgagc tcggcggcgc gcgcgtccac gagcgcggag cgccggccgg agagcagggc
    11221 ggtctggatg ccgatctcgt gcagcatctt gacgccgtgg ccgtcgcgca cgtggaaaac
    11281 cttcatctcg cccccctgga ccaggtagag cgcgccgtcg gtgagcacgc catcgacgtc
    11341 gaacaccatg aaccgcacgc gcctggcgtg cgcaagcgcc tcccgccgcg tgcgcatcaa
    11401 atgaccttcg ctcggaaaag gtcgtgcatg ttcagggcgc cggcaagacc accctgctcg
    11461 tcgacgacca ggagctggtt gatcttgtgt cgctccatca tctcgacggc ttccacggcc
    11521 agcttgccgg cctgtatggt ccgcgggttg cgggtcatca cgtcgggcag cgcgaccgcg
    11581 cgtagatcgg acgagcgctc gagtgctcgc ctcaggtcgc cgtcggtgaa gatgccgacg
    11641 acgcgtcgcg agcggtcgac gaccgccgtc atgcccatgc gcttgcgcga catctcgcgc
    11701 agcgcgtcgc tcagcgacgc gctttcggcg acctgcggca cgtcgtcgcc ggtccgcatc
    11761 acgtcgctca catgcgtgag cagccttctg ccgagcgagc ccccgggatg cgagcgcgcg
    11821 aagtcgtcgg caccgaagcc ccgcgcgtcg agcagcgcga tcgcgagcgc gtctcccagc
    11881 gcgagcgcgg cggtcgtgct cgccgtgggc gccaggttga gcgggcaagc ctcctgcgaa
    11941 acgcgggcgt ccaggcaaac gtcggcctcc ttcgcgagcg ccgaggcgtc gtttcccgtg
    12001 atcgcgatca gcttggcgcc ttgccgcttg atgagcggta tgatcgtgag cagctcgtcg
    12061 ctctcgccgg aatacgagag cgcgaccacg acgtcttcgc gcgtgatcat gccgaggtcg
    12121 ccgtggcttg cttccgctgg gtgcacgaag aacgcgggcg tgccggtgct cgccatcgtg
    12181 gaggcgatct tgcgcgcgat gtgtccggac ttgcccatgc cgctcacgac gacgcgcccg
    12241 cggcacgaaa gcagcagatc gagggcggcg agaaagctct gatcgatgcg cgacgcgagc
    12301 gccgacacgg cgtcggcctc gatgacgagg acttttcggg cgagatcgag cgccgaatcg
    12361 gcggcgccga ccgtgctggc gccggaagag gccattatca tgcgctcagt atatcagagg
    12421 ccctttccac aatcagaaac ttgccttatg cctcaaagac ttccgaatgg gtagcacgct
    12481 cgagctgatc ctgctgttgc tcgccagcgc ggtggtcgcg gtcacggcgt tccggtgggt
    12541 gcacctgccg cctctgctcg ggtatctgct cgtcggcgtg gtgatcggcc cgttcgcgtt
    12601 cggcctgatc ccggactccg agcagctgcg ccgcctggcc gagttcgggg tcgtgttcct
    12661 gatgttctcg atcggcctgg aattcaatct gccgacgttg ttcaccatgc gccgcgcggt
    12721 gttcgggctc ggtctggcgc aggtgctact cacggcgggg accgtcatgg tggtggcgcc
    12781 gctggcggga ttcgcatggg aacctgctct tgcagtaggc ggcgcgctcg ccatgtcatc
    12841 caccgcgatc gtgatgcgca tgctgtcgga gcgcatgcag ctcgataccg accacggacg
    12901 cagcatcgtc ggcgtgctcc tctttcagga tctcgcggtg gtccctctcc tgatcgtgat
    12961 cccggcgatc gcgcacggcg gcgacgtcgc gcgccagctc ggccttgcgc tcgtcaaggc
    13021 cggaatcgtc ctcggtgtcg tgctcttcct cggacaccgg ctgatgcgac cgtggctgca
    13081 tgtggtcgcc ggtcgccgct cgcacgagct cttcatcctc aatgtgctgt tgctgacgct
    13141 cgggctcgcc tggctcaccg agatcgcggg cctgtccctg gcgctgggcg cttttctcgc
    13201 cgggatgctg atcgccgaga cgccgtaccg gcaccaggtg gaggaggaca tcaaaccgtt
    13261 tcgcgaggtg ctgctcggcc tgttcttcgt caccatggga atgaagctcg acgtgcgcat
    13321 cgtggcgcac gaggctccgc tggtgctggt actggtcgtg ctgccggtgg cgttcaagct
    13381 cgcgctcacc gctgcgctcg cccgtttctt cggctcgggg ccgggaagcg cgctgcgcat
    13441 cgggctcgcc atcgcgcacg cgggagagtt cggcctggtg ttgatcgtcc aggccaccga
    13501 tgtcggtctg ctcggcgcgg aattctcgca gctcgtggtc gctgcgatgc tgatctcgat
    13561 gctcgtctcg cccttcattc tccaggcgag cgaccgactc gtcatgcgct gggcgagttc
    13621 ggaatggatg ctgaaatcgc tggagctgca tcgcatcgct gtgcagagcc tggcgaccga
    13681 aaatcacgtc attgtctgcg ggtacggacg caccggtcag cgcctcgcgc acttgctgga
    13741 gcaggaggac atacgctaca tcgccctcga cctcgacccc gagcgggtgc gccaggcggg
    13801 ggccgcgggc gatgcggtcg tgtacgggga cgcctcgcat cgggaagcgc tggtcgcggc
    13861 cggcctggcc cgggccggcg cgctcgtggt cacgttcgcc gacacggccc gggcgctgaa
    13921 ggtcatccac cacgcacacg atgtgaaccc ggcgctgccg gtgctggtac gcacgctcga
    13981 cgacgccgac ctcgatcggc tgctcgccgc cggcgcagcc gaggtggtcc ccgagacttt
    14041 cgagtcgagt ctcatgctcg gctcgcacgc gctcatgctc gtcggcgtgc ccttgacccg
    14101 cgtcgtgcgg cggatccgcg acgtgcgcgc gcaccgctac gatctgctgc gcggcttcta
    14161 tcgcggcgag tcggacgagg cgagcgaccc cgatgaggcg cgatcgcccc gtctgcgctc
    14221 ggtcgccctg catcccggag cgcgcggcgc cggccggcgg ctcggcgagc tcgcgctcga
    14281 tgcgatcggc gtcaccgtgc cggcgatacg gcgtcgcgag acgcgcatgg tccgtcccgg
    14341 cccggagacc gagctcgccg agggcgacgt cgtcgtgctt ctgggcgggc cggagcagct
    14401 cgccgccgcg gaggcgcggc tactcgggca ctgaaaaaag cgaaagcccg cgcgaggcgg
    14461 gctttctgcg ggcagcctgc cgcttagagc ggctggcaga agttgtacgt ggtccccact
    14521 tcgccgtagt tgttgttggg gatcgccggc gcgttggtgg gaacatcggc gttcgcggcg
    14581 ccggtgaatg cgcggagctg gccggtgttc ggccggccgt cgtccatctg cgcgtcgac
//
LOCUS       DATDMW010000361        14905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_231202, whole genome shotgun sequence.
ACCESSION   DATDMW010000361 DATDMW010000000
VERSION     DATDMW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14905
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231202"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VI319_15120"
     CDS             <1..498
                     /locus_tag="VI319_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6865224.1"
                     /translation="AVLDGGWGQWVAENRPVGPDVPAPVAARWSPTRRPEHVDAAFVR
                     AHLGELTLLDARAPDRYRGQNETLDPVGGRIPGARNRFFRDNLDASGRFKRPEALRAE
                     LAAAAGDGDVVHYCGSGVSACHNVLAMAVAGLPTGRVYAGSWSEWCADPSRPTASGEP
                     RPGER"
     gene            606..1847
                     /locus_tag="VI319_15125"
     CDS             606..1847
                     /locus_tag="VI319_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY6865225.1"
                     /translation="MRVLLIEDSDAERARIVAQLREAMPEAQVEQWPPRERGRPPRDF
                     YWRRYDALLLSDAPCGEDGLDWLRGFHAASSDLPPTVILAGLGGEDHAVRAVKAGAAD
                     FLRKTEITGTRLAIALREARLDVAEAYNPADTQTRSQPLDISTIGMPARKDAVRVRGY
                     KILRQIGEGGMAKVYLAEREETRLQLVLKVLDRTVLRDEQFLERFRRECRIISKIQNE
                     HVVAIYDHGVSDDCAFLAMEYFGGGDLKARIARGLSSMEALKVLMQLAKALDAVHSAG
                     VMHRDIKPQNIMFREGNRLALVDFGLAKEVDGRSTVTHAGLVLATPLYMSPEQYESAR
                     QDERSDLYSVGVILYEMLTGKLPFTASNAPALAYQHVNSPVPRLPQRLAGYQGIVDRL
                     MAKRPDDRFQSARELFAYIAH"
     gene            1864..2658
                     /locus_tag="VI319_15130"
     CDS             1864..2658
                     /locus_tag="VI319_15130"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY6865226.1"
                     /translation="MKQYLDLMRHVLEHGHEKSDRTGTGTLSVFGWQMRFDLAAGFPL
                     LTTKKVHLKSIVYELLWFLRGDTNVRWLQQHGVTIWDEWADEKGDLGPVYGSQWRSWP
                     GPDGATIDQIAHVVRSIRTRPDSRRHMVTAWNPAEIPRMKLPPCHALFQFYVADGALS
                     CQLYQRSADVVLGVPFNIASYALLTMMVAQVCGLRPHEFVHTFGDAHLYLNHLDQARE
                     QLSRQPRPLPRMRLNPAVNDIFGFRYEDFTLEGYDPHPAIKAPIAV"
     gene            complement(2805..3329)
                     /locus_tag="VI319_15135"
     CDS             complement(2805..3329)
                     /locus_tag="VI319_15135"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY6865227.1"
                     /translation="MSAENGTMGGGRRLNPLVAGAAVSVMVFSLVGIAAMTGMLPGAM
                     SEKGVEVAPAPVAPRVAACPSCGTVESIREVELKGEATGLGAVAGGITGAVVGHQVGN
                     GRGQDAMTVLGAAGGAYAGNEIEKNVKKHIVYRVTVKMDDGSFRTVSQAAAPSFSVGD
                     KVRIVQGALHAAKS"
     gene            complement(3613..4965)
                     /locus_tag="VI319_15140"
     CDS             complement(3613..4965)
                     /locus_tag="VI319_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY6865228.1"
                     /translation="MSKPSILLVDDDADILRLVSMRLTGAGYSVVAVDSAEKALAAFA
                     VSRPQVVVTDLKMSGMDGMALFEEIRKQAPTLPVILLTAHGTIPDAVAATQRGVFGFL
                     PKPFDGKLLLEQVEQALRVSGVGAGAAAGGDRDWLGEFITRSSAVEETLRQARLVAQS
                     DASVFIRGASGTGKELLARAIHRASKRADKPFVAVNCAAIPENLLESELFGHRKGSFT
                     GAMYDHKGLFPTADGGTLFLDEIGDMPLALQAKLLRVLQDGEVRPVGATQSVPVDVRI
                     ISATHRDIDAQLKAGQFREDLYYRLNVVSVSLPPLAERREDVVPLAAHFLRATAERYG
                     KDVQAFAPDALELLIAAPWPGNVRQLLNLVEQVVALSSSRIVPASLVQQALREEPKPL
                     ASLEEARRGFEQEYLVRILRMTRGNVTQAAKLAHRNRTEFYKLLERHRLEPRMFKPEK
                     "
     gene            complement(4962..5411)
                     /locus_tag="VI319_15145"
     CDS             complement(4962..5411)
                     /locus_tag="VI319_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865229.1"
                     /translation="MAACNHLPLPLSDDFESRDVTSLIAQANRFAGLQGDDQKKDMNA
                     ATQSFAKDRSAVSRIRLALLLSLPGTPFTDEARALTLLEPLASGGGAAKAAPLRQFAV
                     LVHAELGERVREQRRSALLREQSAQMKEQLDALRAIERSIIERGKQK"
     gene            complement(5455..6903)
                     /locus_tag="VI319_15150"
     CDS             complement(5455..6903)
                     /locus_tag="VI319_15150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6865230.1"
                     /translation="MKVALDFPQRYYPKSFLKLLLFAFGAVVLPLIVAFVNAAVYVDR
                     LADQSQSAVAQAAQAGRASRQLMEQVTALERVVRQYLVLGDGGLIDDYRQLRAGFKST
                     TSELSLLPLDEVQLQELNRTIDKEQELYDMLVKTPLRPRESAALIEGYVSLSDLARGV
                     LEVSNKLIDRELERMRETGAHAQRILWWQLIATIPVGVLIAIGVTFLIARPIQQLDHA
                     IRRLGAGQFSGDIRVKGPEDLEYLGKRLDWLRQRLAELEQQQRLFLRHVSHELKTPLT
                     SLREGSELLADGATGRLTEQQLEIAGILRQKSLQLQGLIEELLDYQRAQESVARLEFA
                     PVRLDAVIRRVVEDYRLAAARRAIRTQLHLEPVMLRGDGEKLRVVVDNLVSNAVKYSP
                     DGGTITLALRREHESVILDVSDTGPGIPAEDHGRIFDWFFRGEHGHHGRVQGSGLGLA
                     IAKELVSAHSGRIEVVNDGGAGGHFRVSLPTT"
     gene            6948..7451
                     /locus_tag="VI319_15155"
     CDS             6948..7451
                     /locus_tag="VI319_15155"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY6865231.1"
                     /translation="MNRPVVSLLVAVARNGVIGRNNDLPWHLPEDLKRFKALTLGYPV
                     VMGRRTYESIIERMGRPLPGRDNIVVTRRAGYAAPGCRVVHSLEAALAAASGAKKIFV
                     IGGADIFALALPLANRLDVTEIDADVEGDVVFPAYDRAEWREVSREAGAGAADLRYDF
                     VVYERAK"
     gene            complement(7497..8696)
                     /locus_tag="VI319_15160"
     CDS             complement(7497..8696)
                     /locus_tag="VI319_15160"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEY6865232.1"
                     /translation="MQMRAGLAKKTIKSVRQQAYEANQVFDSHPESSLDFEKVQQAVR
                     DGYSKPFLLIDSAIVREKARRFAAAMPRVRPHYAVKANPDPRVLQALIEEGAGFEIAS
                     IAELDVLLALGVPADEIFYSNPLRSRDYIEYAASKGVAWFVVDSPDELKKVHAIKPDA
                     KLYLRIDTPNIGSDWPLGGKFGAHGAETREIVALAARTKADLAGVAFHVGSQCRNPEN
                     WRVGIEKARQVFDLMQKAGLNPRLLNIGGGFPVRHVKPIPSIEVIGDVVNRALEAFPA
                     HIKVIAEPGRYMVSDAGYFVCRVIGTATRGGKRWMHLDAGLFGGIIETTEGLKYKIRT
                     DRSGPDIPWNVAGPTCDSIDVVMRDELLPSDLEEGDFVYLKNAGAYTTAYASNFNGFP
                     LPEVRVI"
     gene            complement(9131..9628)
                     /locus_tag="VI319_15165"
     CDS             complement(9131..9628)
                     /locus_tag="VI319_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865233.1"
                     /translation="MKHARIAVLFGISVGFAAALAPVSARADDVVRWKTVIGIIQAGN
                     LVGNIGGGGQPWSTTGGEAKVNLRDGHLEFNVRGLVLAGGNTIGTPGAINQVKGTLVC
                     NPGPAQAVVDGAPVALSPTGDAQFSGDLGTLPADCAGGNVAFLVRIVPADRWIANGAV
                     RQGND"
     gene            complement(9724..10287)
                     /gene="dcd"
                     /locus_tag="VI319_15170"
     CDS             complement(9724..10287)
                     /gene="dcd"
                     /locus_tag="VI319_15170"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY6865234.1"
                     /translation="MTIKSDRWIRRMAMQGMIEPFEAGQVKEVDGRRIVSYGTSSYGY
                     DIRCSTEFKIFTNINSTIVDPKAFDEKSFVDYRGDVCIIPPNSFALARTVEFFRIPRN
                     VLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPARIYANEGVAQVIFIE
                     SDEVCETSYRDRGGKYQGQKGVTLPKI"
     gene            complement(10306..11394)
                     /gene="apbC"
                     /locus_tag="VI319_15175"
     CDS             complement(10306..11394)
                     /gene="apbC"
                     /locus_tag="VI319_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEY6865235.1"
                     /translation="MAVTQEQIHAALSQIVDPNTGKDFASTKSARNIRIENGSVTLDV
                     ELGYPAKTQVEPIRKLLSGGLRAVPGVESVKVNVTSKIVPHAVQRGVKLVPGVKNIVA
                     VASGKGGVGKSTTAVNLALALAAEGARVGVLDADIYGPSQPMMLGITGRPESKDGKSL
                     EPMTGHGVQAISIGFLIDVDTPMVWRGPMVTQALEQLLKDTRWEDVDYLVVDMPPGTG
                     DIQLTLAQKVPVTGAIIVTTPQDIALIDARKGLKMFEKVGIPILGIVENMSTHICSSC
                     GHEEHIFGAGGAERMAKEYGVELLGSLPLDISIREQADSGKPTVVADPDGRIAEAYRA
                     IARRVAVRIAERQKDMSLKFPNIVVQNT"
     gene            11562..12887
                     /locus_tag="VI319_15180"
     CDS             11562..12887
                     /locus_tag="VI319_15180"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY6865236.1"
                     /translation="MADNTNRTFICSVLFLDIVEYSRRSVTEQLKLKEQFNTTLSEAI
                     SGVAADDRIILDTGDGAAVSFLGDPEDAMFVALSMRDAIASQSDGGPLLRVRFGINLG
                     PVRLIKDINGQPNIIGDGINVAQRIMTFAEPGQILVSRSYYDIIARLSEDYANLFHYE
                     GARTDKHVREHEVYAVGAAPRRLRSRTQPPPPRSRSSFPGFRLDRLAGGLAVDWKLLV
                     VAPLVFLFIVGSAFAVRSYRKAEQAALVAVAPASRSARAEAPRVTPVEDTRPAPAPVE
                     ERPVKPAPKPKVEAKPKVEAKPTREDLAARREKERQEAAAKKEKERQEAAARKEQEKQ
                     ELAAKKEKEKQDLAAKKEKDRLELAALAERKAAKEREGPPGSLQFSVLPWGEVFVDGK
                     KLGVSPPLHTVPITPGKHQLEVRNGSFPAHRQTLNVKPGESIIVSHRFR"
     gene            12904..13299
                     /locus_tag="VI319_15185"
     CDS             12904..13299
                     /locus_tag="VI319_15185"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HEY6865237.1"
                     /translation="MSRRGFALVLALVLVAGCSSRPMLGISRAIGTSSNKDLAGGIRA
                     YESADYEESERLLRRSLDEGLTFQRDEITAHKYLAFTYCVTNEERKCREQFRKVLELD
                     PRFELDKSEAGHPIWGPVFRSTRASLNKK"
     gene            13310..14533
                     /locus_tag="VI319_15190"
     CDS             13310..14533
                     /locus_tag="VI319_15190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6865238.1"
                     /translation="MSTQRSIGRYEIQAELGRGAMGVVYRANDPLLSRTVAIKTINMD
                     LEQDGMEEYEARFYQEAKAAGGLNHPNIVIVYDIGKTGNLAYMAMEYIDGVELRSMLS
                     QEQPLPVAQALDIAAQVAEGLGYAHEHQVVHRDVKPANILITPNGRAKIADFGIARMR
                     SAETQTQTGVILGSPKYLSPEQVVGKRADHRSDVFSLGVILYQCLTGKTPFNGDGVSA
                     LMYQITNHNPAPPSTMNPQVPVMLDYIIAKALAKSVDERYQSAADFAADLRECRKTLE
                     PPMEASNPRLSTSGSNPVLSVTQTVVLDRSQTLKPKEPAAAALPDAPAPPAEVVPTRG
                     LSRAFDSTEATQRLLAQTLDPQGEVAPQDPETSGAMVLPYGLRRARPWGDRENKILAA
                     TVMAALAIAAWIISA"
     gene            14610..>14905
                     /locus_tag="VI319_15195"
     CDS             14610..>14905
                     /locus_tag="VI319_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY6865239.1"
                     /translation="MPRRLFVTTALPYANGAFHIGHIMEYIQADIWVRFQRMQGNAVH
                     FVGADDAPGAPIMLAAEKAGKSPEEFVAEIARGRKQYLDGFHIEFDNWHSTHSK"
BASE COUNT         2382 a         5154 c         5037 g         2332 t
ORIGIN      
        1 gccgtgctcg acggcggctg gggccagtgg gtcgcggaga accggcccgt cggccctgat
       61 gtgccggcgc cggtcgcggc tcgctggtcg ccgacgcgcc gtcccgagca cgtcgatgcc
      121 gcgttcgtgc gcgcgcatct cggcgagctg acgctgctcg acgcccgcgc gcccgaccgc
      181 taccgcgggc agaacgagac cctcgatccg gtcggcggcc ggattcccgg cgctcgcaac
      241 cgcttcttcc gcgacaacct cgacgcctcc ggccgcttca agcgcccgga agcgctccgg
      301 gccgagctcg ccgccgccgc cggagacggc gacgtcgtgc actactgcgg ctcgggcgtc
      361 tccgcctgcc acaacgtgct cgcgatggcc gtcgcgggcc tgccgaccgg ccgcgtctac
      421 gccggatcct ggagcgagtg gtgcgcggac ccgtcgcgtc cgacggcgtc gggcgagccg
      481 cgccccgggg agcgctagtc ccgcgtcgcc ggcggcctcg cccgtccggg gttggcgcga
      541 ggcgcccccg agggcaaagt ccgcatccgg tccgggcccc cagcggttat gatcgcgcac
      601 cgcccatgag agtgctcctc atcgaggatt ccgacgccga gcgcgcgcgg atcgtcgccc
      661 agctgcgcga ggcgatgccc gaagcgcagg tcgagcaatg gccgccgcgc gagcgcggcc
      721 gtccgccgcg cgacttctac tggcgccgct acgacgcgct gctcctgtcc gatgccccgt
      781 gcggcgagga cgggctggac tggctgcgcg gattccacgc cgcgagctcg gacttgccgc
      841 cgaccgtcat cctcgccggc ctggggggcg aggaccacgc ggtgcgcgcg gtcaaggcgg
      901 gcgccgccga tttcctgcgc aagaccgaga ttacggggac gcggctggcg atcgccctgc
      961 gcgaggcccg gctcgacgtg gccgaggcgt acaaccccgc ggacacgcag acgcgctccc
     1021 agccactcga catttccacg atcggaatgc ccgcgcgcaa ggacgccgtg cgcgtgcgcg
     1081 gctacaagat cctgcgccag atcggcgaag gcgggatggc caaggtctat ctcgccgagc
     1141 gcgaggagac gcgcctgcag ctcgtgctca aggtgctcga ccggacggtg ctgcgcgacg
     1201 agcagttcct cgagcgcttt cgccgcgagt gccggatcat ctcgaagatc cagaacgagc
     1261 acgtcgtcgc catctacgat cacggcgtca gcgacgactg cgcgttcctc gcaatggaat
     1321 acttcggcgg gggcgacctg aaggcgcgca tcgctcgcgg cctgtcgtcg atggaggcgc
     1381 tcaaggtcct gatgcagctc gccaaggcgc tcgacgccgt ccactcggcc ggcgtgatgc
     1441 accgggacat caagccgcag aacatcatgt tccgtgaagg caatcgcctc gcgctcgtcg
     1501 atttcgggct ggcgaaggag gtcgacggcc gcagcaccgt cacgcacgcc gggctcgtcc
     1561 tcgcgacgcc gctctacatg agtcccgaac agtacgagag cgcgcgacag gacgagcgca
     1621 gcgacctgta ctcggtcggc gtgatactct acgagatgct gaccggcaag cttccgttca
     1681 ccgcgagcaa cgcgccggcg ctcgcctacc agcacgtgaa ttcgccggtg ccacggctgc
     1741 cgcagcggct cgcgggctac caggggatcg tggaccgcct catggcgaag cgacccgatg
     1801 accgtttcca gtcggcgcgc gagctgttcg cgtatatcgc gcactgaatt cgccactgag
     1861 gcagtgaagc aatacctcga cctgatgcgc cacgtgctcg agcacgggca cgagaagagc
     1921 gaccgcaccg ggaccggcac gctgtccgtc ttcggctggc agatgcgctt cgatctcgcg
     1981 gcgggcttcc cgctgctgac cacgaagaag gtccacctca agtcgatcgt ctacgagctg
     2041 ctgtggttcc tgcgcggcga caccaacgtc cgctggctgc agcagcacgg cgtcaccatc
     2101 tgggacgagt gggccgacga gaagggcgac ctcgggccgg tgtacgggtc gcaatggcgc
     2161 agctggccgg gccccgacgg cgccacgatc gaccagatcg cccacgtggt gcgctcgatc
     2221 cggacgcggc ccgattcacg ccggcacatg gtgaccgcgt ggaacccggc ggagatcccg
     2281 cggatgaagc tgccgccgtg ccacgcgctg ttccagttct acgtcgcgga cggggccctg
     2341 tcgtgccagc tctaccagcg cagcgccgac gtcgtcctcg gggtgccgtt caacatcgcg
     2401 tcgtatgccc tgctcacgat gatggtcgcg caggtctgcg gcctcaggcc gcacgagttc
     2461 gtccatacct tcggcgacgc gcacctgtac ctcaatcacc tcgaccaggc gcgcgagcag
     2521 ctctcgcgcc agccgcggcc gctcccgcgc atgcggctca acccggcggt gaacgacatc
     2581 ttcgggttcc gctacgagga tttcacgctg gaaggctacg acccgcaccc cgcgatcaag
     2641 gcgccgatcg cggtctagga gccggggatg cgttccccgc ccggctcgcg ccgggcgggt
     2701 cccgctgtat tgatacccgc cgccgcgagg cggcgggcag agcaccgacg tggccgagca
     2761 agggaggggt tgctcgaccg gccgggtaaa gctgtcctgc ctccttacga cttcgccgcg
     2821 tgcagggcgc cctgcacgat gcgcaccttg tcgccgaccg agaacgacgg cgcggccgcc
     2881 tgcgaaacgg tccggaacga gccgtcgtcc atcttcaccg tcacccggta gacgatgtgc
     2941 ttcttcacgt tcttctcgat ctcgttcccg gcatacgcgc cgccggccgc gccgagcacc
     3001 gtcatcgcgt cctggccgcg gccgttgccc acctggtggc cgaccacggc gccggtgatg
     3061 ccgccggcaa ccgcgcccag gcccgtcgcc tcgcccttca gctcgacctc gcggatcgac
     3121 tcgacggtcc cgcagctcgg gcacgcggcc actctcggcg ccaccggcgc cggcgcgacc
     3181 tcgacgccct tctccgacat cgcgccgggc agcatccccg tcatggcggc gatgccgacc
     3241 aggctgaaga ccatcaccga taccgccgca cccgcgacca gcgggttcag ccggcgacca
     3301 ccgcccatcg ttccgttctc ggcgctcatc atctttaccg cctgtcgcca agtcttgtgc
     3361 tgcgaagttt cacggtactg cttcctttcc ctgagtcgag ctggctcttt tgggccaaag
     3421 ttgtggaagc attagcaagg gaggtgccag ccgagctttc tccagtaatt caggtagatg
     3481 aattccgcga tcgagcggcg cggaagggcg gaatgtcgtc cccgcgcgac gcacgtacgg
     3541 cgccccggac gaagggcatg agcgaaaaga ttatttcctg tcgccggcag gcgacagggg
     3601 cgccgcaaaa cgctatttct ccggcttgaa catgcgcggc tcgaggcggt ggcgctccag
     3661 cagcttgtag aactcggtgc ggttccggtg cgcgagcttc gccgcctgcg tgacgttgcc
     3721 gcgcgtcatc cgcaggatgc gcacgaggta ttcctgctcg aaccctcgcc gcgcctcctc
     3781 cagcgaggcg agcggcttcg gctcctcgcg cagcgcctgc tgcacgagcg aggccggcac
     3841 gatccgcgac gacgacagcg ccaccacctg ctccaccagg ttgagcagct ggcgcacgtt
     3901 gcccggccag ggcgccgcga tgagcagctc gagggcatcg ggcgcgaagg cctgcacgtc
     3961 cttgccgtag cgctcggccg tcgcgcgcag aaagtgcgcg gcgaggggca cgacgtcctc
     4021 gcgccgctcg gcgagcggcg gcagcgacac cgagacgacg ttcagccggt aatacaggtc
     4081 ctcgcggaac tggccggcct tgagctgcgc gtcgatgtcg cggtgcgtgg ccgagatgat
     4141 gcgcacgtcc accggcacgg actgcgtcgc gccgacggga cgcacttccc cgtcctgcag
     4201 cacccgcagg agcttcgcct gcagggcgag cggcatgtcg ccgatctcgt cgaggaagag
     4261 cgtcccgccg tcggcggtcg ggaacagccc cttgtggtca tacatcgcgc ccgtgaacga
     4321 gcccttgcga tggccgaaca gctcggactc gagcaggttc tccgggatcg ccgcgcagtt
     4381 gaccgcgaca aagggcttgt cggcgcgctt gctcgcgcgg tgaatggcgc gcgcgagcag
     4441 ctccttgccg gtgccgctcg ccccgcggat gaagacgctc gcgtcggact gggcgacgag
     4501 gcgcgcctgg cgcagcgtct cctcgaccgc ggagctgcgc gtgatgaatt cgcccagcca
     4561 gtcgcggtcc ccgcccgcgg cggcgcccgc gccaacgccg gaaacccgca gcgcctgctc
     4621 gacctgctcg agcagcagct tgccgtcgaa gggcttcggc aggaaaccga acacgccgcg
     4681 ctgcgtcgcg gcaacggcat cgggaatcgt gccgtgcgct gtgaggagga tcacggggag
     4741 cgtgggcgcc tgctttcgga tctcctcgaa cagcgccatg ccgtccatgc cgctcatctt
     4801 gaggtcggtc accacgacct gcgggcgcga cacggcgaag gcggcgagcg ccttctcggc
     4861 gctgtcgacc gcgacgacgc tgtagccggc gcctgtcagg cgcatggaga cgagccgcag
     4921 gatgtcggca tcgtcgtcga ccagcaggat ggacggcttg ctcatttttg ctttcctcgt
     4981 tcgatgatac tgcgctcgat cgcgcgcagc gcgtcgagct gttccttcat ttgcgccgac
     5041 tgctcgcgca gcagcgccga gcggcgctgc tcgcgcacgc gttcccccag ctcggcgtgg
     5101 accagcacgg cgaactgccg caatggcgcc gccttcgcgg ccccgccgcc ggacgcgagc
     5161 ggctcgagca gcgtgagcgc ccgggcctcg tccgtgaacg gggtgccggg cagcgacagg
     5221 agcagcgcga gacggatccg cgacacggcg ctgcgatcct tggcgaagct ctgcgtcgcg
     5281 gcgttcatgt cctttttctg atcgtcgccc tgcagcccgg cgaaccggtt cgcctgcgca
     5341 atcagcgacg tgacgtcccg ggactcgaag tcatccgaca gcggcaaggg aagatggttg
     5401 cacgcggcca ccaggaggca caccagcccg agcgcggata gattcttcat cgacctaagt
     5461 cgtcggcaag ctgacacgga agtggccccc ggcgcccccg tcgttaacta cttcgatgcg
     5521 cccggagtgg gcggatacga gctctttcgc gatggcgagg ccgagaccgc tgccctggac
     5581 gcgcccgtgg tgcccgtgct cgccgcggaa gaaccagtcg aagatccgtc cgtggtcttc
     5641 ggccgggatc ccggggccgg tgtccgagac gtccaaaatg acgctctcgt gctcgcgcct
     5701 caatgcgagc gttatcgtac cgccgtccgg agaatatttc acggcgttcg agacgagatt
     5761 gtccactacg acacgcagct tctcgccgtc gccgcgcagc atgaccggct cgaggtgcag
     5821 ctgcgtgcgg atggcgcgcc gcgcggcggc gagccggtaa tcctcgacca cccgccggat
     5881 cacggcgtcg agccgcaccg gggcgaactc caggcgcgcc acgctctcct gcgcccgctg
     5941 gtagtcgagc agctcctcga tcaggccctg gagctgcagg ctcttctggc gcaggatccc
     6001 ggcgatctcc agctgctgct cggtgaggcg gccggtcgcg ccgtcggcga gcagctccga
     6061 gccctcgcgc agcgaggtga gcggcgtctt cagctcgtgc gagacatgcc gcaggaacag
     6121 gcgctgctgc tgctcgagct cggcgaggcg ctggcgcagc cagtcgagcc gcttgcccag
     6181 gtactcgagg tcctcgggcc ccttcacgcg gatgtcgccg gagaactggc cggcgcccag
     6241 gcgccggatg gcgtggtcga gctgctggat cgggcgcgcg atgaggaagg tcacgccgat
     6301 cgcgatcagc acgccgaccg ggatggtcgc gatcagctgc caccagagga tgcgctgcgc
     6361 gtgcgcgccg gtttcgcgca tgcgctcgag ctcgcggtcg atcagcttgt tgctgacctc
     6421 gagcacgccg cgcgcgagat cggagagcga tacgtagccc tcgatcagcg ccgcgctctc
     6481 gcgcggacgc agcggcgtct tgaccagcat gtcgtacagc tcctgctcct tgtcgatcgt
     6541 acggttgagc tcctgcagct gcacctcgtc gagcggcagc agcgacagct cgctcgtcgt
     6601 ggacttgaat cccgcgcgca gctgccggta gtcgtcgatc aggccgccgt cgccgagcac
     6661 gaggtattgg cgcaccacgc gctcgagcgc ggtcacctgc tccatgagct ggcggctcgc
     6721 gcgaccggcc tgcgccgcct gggcgaccgc gctctggctc tggtccgcaa gtctatccac
     6781 gtacaccgcg gcgttcacga aagcgacgat cagaggcagc accaccgcgc cgaacgcaaa
     6841 cagcagcagc ttgaggaagg acttggggta gtagcgctgc gggaagtcga gcgcgacttt
     6901 catcgcggtc attataatgc caggcgtttt ccgacccccg ccgcgccgtg aatcgtcccg
     6961 tcgtctccct gctcgtcgcg gtcgcgcgca acggcgtgat cgggcggaac aacgacctgc
     7021 cctggcacct tcccgaggac ctgaagcgct tcaaggcgct gacgctggga tatcccgtcg
     7081 tcatgggacg caggacctac gaatcgatca tcgagcgcat gggcaggccg cttccgggcc
     7141 gcgacaacat cgtcgtgacg cggcgcgccg gctacgccgc gccgggctgc cgcgtcgtgc
     7201 attcgctcga agcggcgctc gccgccgcgt cgggcgccaa gaagatcttc gtcatcggcg
     7261 gcgcggacat tttcgcgctc gcgcttccgc tcgcgaaccg gctcgacgtg accgagatcg
     7321 acgccgacgt cgagggcgac gtcgtcttcc ctgcctacga ccgcgccgag tggcgcgagg
     7381 tcagccgcga ggcgggcgct ggcgccgcgg acctgcgcta cgacttcgtc gtctacgagc
     7441 gcgcaaaata agaagggcgc gccggagcgc gcccttctcc cgcctggcgt cccccgtcag
     7501 atgacgcgca cctcgggcag cggaaacccg ttgaaattgc tcgcgtacgc cgtggtgtag
     7561 gcgcccgcat tcttgaggta gacgaagtcg ccctcctcga ggtccgaggg cagcagctcg
     7621 tcgcgcatga cgacgtcgat cgagtcgcag gtcggccccg cgacgttcca cgggatgtcg
     7681 ggccccgagc ggtcggtacg gatcttgtac ttcaggccct cggtcgtctc gatgatgccg
     7741 ccgaacaggc cggcgtcgag gtgcatccag cgcttgccgc cgcgggtcgc cgtgccgatc
     7801 acgcggcaca cgaaatagcc ggcgtccgag accatgtagc gcccgggctc ggcgatcacc
     7861 ttgatgtgcg ccgggaacgc ctcgagcgcc cgattcacga cgtcgccgat cacctcgatc
     7921 gagggaatgg gcttcacgtg gcgcaccggg aagccgccgc cgatgttcag caggcgcggg
     7981 ttgaggccgg ccttctgcat caggtcgaac acctggcgcg ccttctcgat gccgacgcgc
     8041 cagttctcgg gattgcggca ctgcgagccc acgtggaagg cgacgccggc gagatcggcc
     8101 ttcgtcctcg ccgcgagcgc cacgatctcg cgcgtctcgg cgccgtgcgc gccgaatttg
     8161 ccgcccaggg gccagtcgct gccgatgttg ggcgtgtcga tgcgcagata gagcttggcg
     8221 tcgggcttga tcgcgtggac cttcttcagc tcgtcggggc tgtcgacgac gaaccacgcg
     8281 acgcccttcg acgcggcgta ctcgatgtag tcgcgcgacc tgagggggtt cgagtagaag
     8341 atctcgtccg cggggacgcc gagcgcgagc agcacgtcga gctcggcgat cgaggcgatc
     8401 tcgaagcccg cgccctcttc gatcagggcc tgcagcacgc gggggtcggg gttggctttc
     8461 accgcgtagt gcgggcgcac gcgcggcatt gcggcggcaa agcggcgcgc cttttcgcgg
     8521 acgatcgcgg aatcgatcag gaggaacggt tttgaatagc cgtcgcggac ggcctgctgg
     8581 actttttcga agtccaggct cgactcgggg tgcgagtcga agacttggtt tgcttcgtat
     8641 gcctgctgtc ggacggattt gatggttttt ttggctagac cggcacgcat ctgcatcgca
     8701 atggctcctt acggacttac gtaacgggaa tgtcgagcgc gaagaaatcc tgcgagccgt
     8761 acaccttact cttgcttgtc atgatgacct cctgctcctg gtcgaaactg cggtgcaaaa
     8821 ccgcgttgca aaaaaccaat ttggcaaaag catcgggccc gggtttgacc gtccgctgag
     8881 cagacaaccg aaccggagcc cgtttgcgcg aattataggt ctgcccctcc cagggtcaag
     8941 cggaattttc cgcattcgct gcaggagcgg ccagtgtccg gacgctccct ccactattta
     9001 tggtaggggc atcccgggac aaacgcaata cctggtgtac cgcgagccgg cccgccggcc
     9061 ggcgccttac aaacccttac aaaaaaacgg cgccggtcca gccggcgccg cggcgatagt
     9121 gccgggcgag ctagtcgttg ccctggcgca ccgctccgtt ggcgatccag cgatccgccg
     9181 gaacgatgcg cacgaggaag gcgacgttgc cgcccgcgca gtcggccggg agcgtcccga
     9241 gatccccgct gaactgagcg tccccggtcg ggctcaaggc gaccggcgcg ccgtccacga
     9301 ccgcctgggc cgggcccgga ttgcagacca gcgtgccctt cacctggttg atcgcgcccg
     9361 gtgtaccgat cgtgttcccg ccggcgagca ccagaccccg cacgttgaac tccaggtgcc
     9421 cgtcgcgcag gttgaccttg gcctcgcccc cggtcgtcga ccagggctgg ccgcccccgc
     9481 cgatgtttcc gaccaggttt ccggcctgga tgatgccgat gaccgtcttc cacctcacca
     9541 cgtcgtccgc ccgggccgag accggggcga gcgccgcagc aaaacccacg gaaatgccga
     9601 aaaggactgc gatgcgcgcg tgcttcatgg ctgtctcctc aagggggtgc accgggccgg
     9661 acggccgcgg cgagaggtaa acagccggga cggcgggaca ttccgccgtc atattcccgg
     9721 aggtcagatc ttcggcagcg tgacgccctt ctgtccctgg tacttgccgc cgcggtcgcg
     9781 gtacgaggtc tcgcacacct cgtcggactc gatgaagatc acctgggcga cgccctcgtt
     9841 ggcatagatc ctggcgggca gcggcgtcgt gttggagaac tcgagcgtca cgtgcccctc
     9901 ccactccggc tcgagcggcg tgacgttgac gatgatgccg cagcgcgcgt aggtcgactt
     9961 cccgaggcag atggtgagca cgttgcgcgg aatgcggaag aactcgaccg tgcgcgcgag
    10021 cgcgaacgag ttcggcggga tgatgcagac gtcgccgcgg tagtcgacga agctcttctc
    10081 gtcgaaggcc ttggggtcga cgatggtcga gttgatgttg gtgaagatct tgaattcggt
    10141 cgagcagcgg atgtcgtagc cgtagctcga ggtgccgtag gagacgatgc ggcggccgtc
    10201 gacctccttc acctggcccg cctcgaacgg ctcgatcatg ccctgcatcg ccatccgccg
    10261 gatccagcgg tcggacttga tggtcatcgc tccctgaccg cctttctagg tgttctgcac
    10321 cacgatgttc ggaaatttca gcgacatgtc cttctgccgc tcggcgatgc gcaccgcgac
    10381 ccggcgcgcg atggcgcggt aggcctcggc gatccgtccg tccggatcgg ccaccacggt
    10441 gggcttgccc gagtcggcct gctcgcggat cgagatgtcg agcggcaggg acccgagcag
    10501 ctcgaccccg tactccttcg ccatgcgctc ggcgccgccg gcgccgaaga tgtgctcttc
    10561 gtggccgcag ctcgagcaga tgtgagtgct catgttctcg acgatgccga ggattgggat
    10621 gccgactttc tcgaacatct tgagcccctt gcgcgcgtcg atgagggcga tgtcctgcgg
    10681 cgtcgtgacg atgatcgcgc ccgtgaccgg gaccttctgc gcgagcgtca gctggatgtc
    10741 cccggtcccc ggcggcatgt cgacgacgag gtagtcgacg tcctcccagc gcgtgtcctt
    10801 cagcagctgc tcgagcgcct gcgtcaccat cggcccgcgc cacaccatcg gcgtgtcgac
    10861 gtcgatcagg aagccgatcg agatcgcctg cacgccgtga ccggtcatcg gctcgagcga
    10921 tttgccgtcc ttcgactccg ggcgcccggt gatgccgagc atcatcggct gcgacggccc
    10981 gtagatgtcg gcgtcgagca cgccgacgcg cgcaccttcc gcggcgagcg cgagcgccag
    11041 gttcaccgcg gtggtcgact tgcccacgcc gcccttgccg ctcgccacgg cgacgatgtt
    11101 cttgacgccg gggacgagct tgacgccgcg ctgcaccgcg tgcgggacga tcttgctcgt
    11161 gacgttgacc ttgaccgatt ccacgcccgg gaccgcgcgc agcccgccgg agagaagctt
    11221 gcggatgggc tcgacctggg tcttcgcggg gtagccgagc tcgacgtcga gcgtcaccga
    11281 gccgttctcg atcctgatgt tgcgggccga cttggtcgac gcgaaatcct tgcccgtgtt
    11341 cggatcgacg atctggctca acgcggcgtg aatttgttcc tgagtgacgg ccatggcggg
    11401 tgcgattccg gcaatcgagg tgccgggaca caatggattg atattctacg cctttccgat
    11461 gcctttcgaa gctcggtccg gggtagaatc gcgtacagcc cggccagact cggcgggcgc
    11521 gcgtcggagg gacgcgcgcg gggggaaaag agacaagagc aatggctgac aacaccaatc
    11581 ggaccttcat ctgcagcgtc ctgtttctcg acatcgtcga gtattccagg cgctcggtga
    11641 ccgagcagct gaagctcaag gagcagttca acaccacgct gtcggaggcg atctcggggg
    11701 tcgcggccga cgaccgcatc atcctcgaca ccggagacgg cgccgccgtc agcttcctcg
    11761 gcgaccccga ggacgcgatg ttcgtcgcgc tgtcgatgcg cgacgcgatc gcgagccagt
    11821 cggacggcgg cccgctgctg cgcgtgcgct tcggcatcaa cctcggtccg gtgcgcctca
    11881 tcaaggacat caacggccag cccaacatca tcggcgacgg catcaacgtc gcgcagcgca
    11941 tcatgacctt tgccgagccc ggccagatcc tggtctcgcg ctcgtactac gacatcatcg
    12001 cgcggctgtc ggaggactac gccaacctgt tccactacga aggcgcgcgc accgacaagc
    12061 acgtgcgcga gcacgaggtg tacgcggtcg gcgcggcgcc gcgccggctg cgctcgcgca
    12121 cgcagcctcc gccgcctcgg agccgctcgt ccttcccggg cttcaggctg gaccggctcg
    12181 cgggcggcct cgccgtcgac tggaagctgc tcgtggtcgc gcccctggtg ttcctgttca
    12241 tcgtcggctc ggcgttcgcg gtgcgcagct accgcaaggc cgagcaggcc gcgctggtcg
    12301 cggtcgcgcc cgcttcgcgc agcgcgcgcg ccgaggcgcc gcgcgtgacg ccggtggaag
    12361 acacgcgccc ggcgccggcc cccgtggagg agcgcccggt caagcccgcg cccaagccga
    12421 aagtcgaggc caagccgaaa gtcgaggcca agccgacgcg cgaagacctc gccgccaggc
    12481 gcgagaagga gcggcaggag gcggcggcga agaaggagaa ggaacggcag gaagccgcgg
    12541 cccggaagga gcaggagaag caggagctcg ccgcgaagaa agagaaggag aagcaggacc
    12601 tggccgcgaa gaaggaaaag gaccggctcg agctcgcggc gctcgcggaa aggaaagcgg
    12661 ccaaggaaag agaggggcct cccggatcgc tgcagttctc ggtgctcccg tggggcgagg
    12721 tgttcgtcga cggcaagaag ctcggcgtga gcccgccgct gcataccgtg ccgatcacgc
    12781 cgggcaagca ccagctcgaa gtgcgcaacg ggtcgttccc ggcgcaccgg cagacgctca
    12841 acgtgaagcc cggcgagagt atcattgtca gccaccggtt tcgctgaacg agggaggacg
    12901 gcgatgtcgc gccgagggtt tgcgctggtt ctggcgctcg tgctggttgc gggatgcagc
    12961 tcgcgtccca tgctgggaat ctcgagggcg atcggcacgt cctcgaacaa ggacctcgcc
    13021 ggcgggatcc gcgcctacga gagcgccgac tacgaggaat ccgagcgcct gctcaggcgc
    13081 agcctcgacg aaggcctcac cttccagcgc gacgagatca ccgcgcacaa gtacctcgcg
    13141 ttcacgtact gcgtgaccaa cgaggagcgc aagtgccgcg agcagttccg caaggtcctg
    13201 gagctcgacc cgcgcttcga gctcgacaag agcgaagccg gccacccgat ctgggggccc
    13261 gtgttccgca gcacccgcgc cagcctgaac aagaaatagc gcgctcggca tgagcaccca
    13321 gcggagcatc ggccgctacg agatccaggc ggagctgggc cgcggcgcca tgggcgtcgt
    13381 ctaccgcgcg aacgatccgc tgctctcgcg caccgtggcg atcaagacga tcaacatgga
    13441 tctcgagcag gacgggatgg aggagtacga ggcccggttc taccaggagg cgaaagccgc
    13501 cggcggcctg aaccatccca acatcgtcat cgtctacgac atcggcaaga ccggaaacct
    13561 cgcctacatg gcgatggagt acatcgacgg ggtcgagctg cggtcgatgc tgtcgcagga
    13621 gcagccgctg ccggtggcgc aggcgctcga catcgccgcc caggtcgccg aggggctcgg
    13681 ttacgcgcac gagcaccagg tcgtgcaccg cgacgtgaag ccggcgaaca tcctgatcac
    13741 gccgaacggc cgcgccaaga tcgccgactt cggcatcgcg cgcatgcgct cggccgagac
    13801 gcagacgcag accggcgtga tcctcggctc gccgaaatac ctgtcgcccg agcaggtcgt
    13861 cggcaagcgc gccgatcacc gctccgacgt gttctccctg ggcgtcatcc tgtaccagtg
    13921 cctgacgggc aagacgccgt tcaacggcga cggcgtcagc gccctgatgt accagatcac
    13981 gaaccacaac ccggcgcccc cgagcacgat gaacccgcag gtgccggtga tgctcgacta
    14041 catcatcgcc aaggcgctgg cgaagtcggt cgacgagcgc taccagagcg ccgccgactt
    14101 cgccgccgac ctgcgcgagt gccgcaagac gctcgagccg ccgatggagg cgtccaatcc
    14161 gcgactgtcg acgagcggat cgaacccggt gctgagcgtc acgcagacgg tcgtcctcga
    14221 ccgctcgcag acgctcaagc cgaaggagcc ggccgcggcc gctttgccgg acgcgccggc
    14281 gccgccggcc gaggtcgtgc cgacgcgcgg gctctcgcga gcgttcgact cgaccgaggc
    14341 gacgcagcgc ctgctcgcgc agacgctcga cccgcagggc gaagtcgcgc cgcaggaccc
    14401 ggagacgagc ggcgcaatgg tgctgccgta cggtctgcgc cgcgcgcgtc cgtggggaga
    14461 ccgcgagaac aagatcctcg cagcgaccgt gatggcggcg ctcgcgatcg cggcctggat
    14521 catctcggcc tgacgctttt cgccagggcc gccgccccgg gaggcccgag cttccttgga
    14581 gttacaatcg gggcttttcg aaagcccgca tgcctcgccg gctgttcgtc accaccgcgc
    14641 tgccgtacgc caacggcgcc ttccacatcg gccacatcat ggagtacatc caggccgaca
    14701 tctgggtgcg gttccagcgc atgcagggca acgcggtgca cttcgtcggc gccgacgacg
    14761 cgcccggcgc gccgatcatg ctcgcggccg agaaggcggg gaagtcgccg gaggagttcg
    14821 tcgcggagat cgcgcgcggg cgcaagcagt atctcgatgg cttccatatc gagttcgaca
    14881 actggcattc gacccactcg aagga
//
LOCUS       DATDMW010000362         6607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_477616, whole genome shotgun sequence.
ACCESSION   DATDMW010000362 DATDMW010000000
VERSION     DATDMW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477616"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VI319_15200"
     CDS             <1..186
                     /locus_tag="VI319_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865240.1"
                     /translation="GALLAPYTAALQWLCARAGPLTPAALDHALAHPPPDTRELRASD
                     TASSQADAPTAGRRDER"
     gene            complement(263..1453)
                     /locus_tag="VI319_15205"
     CDS             complement(263..1453)
                     /locus_tag="VI319_15205"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY6865241.1"
                     /translation="MPHVPASTALARFRVLDLSRVRAGPTCVRQLADFGADVIKIETP
                     PSVDADTMGGPRHGPDFQNLHRNKRSMTLNLKEREGVGIFMELVKRSDVVVENYRPDV
                     KDRLGIGYESLARVNPKIVLASISGFGQDGPYRDRPGFDQIAQGMGGFMSITGMPGQG
                     PVRAGTAIADISAGLFAALGVLTALLEREASGKGQWVQSSLLMSQIALLDFQAARYLM
                     KGDVPGQAGNDHPTSMPTSAYKTKDGYINIAATGETMWRRVCEGLERKDLLERDDFKG
                     EANRSRNRVALNAEINKATAARTSAEWIEILSRGNVPCGPIYSIDKMFADPQVQHIHA
                     AAKVSSRKLGEFRIVNQAVGLSRTPASIASAPPEIGEHTDEILAELGLGERVAELRTS
                     GAIA"
     gene            1523..2683
                     /locus_tag="VI319_15210"
     CDS             1523..2683
                     /locus_tag="VI319_15210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6865242.1"
                     /translation="MSARDQDLREIRDAVAKLCASFPMEYWRALDRERKYPTEFVRAL
                     TGAGWLSCLIPESYGGAGLGLRAAAAVLEEVQRSGANGAACHAQMYTMGTVLRHCSEA
                     QKRAILPKLASGELRLQAFGVTEPTAGTDTSSIRTAAVRKGDRWVVNGQKVWTSRAEH
                     SDLMLLLARTTPREQVAKRTDGLSIFLVDMKAALGKGLSIRPIRTMINHATTEVFFDN
                     LEIPAENLVGEEGKGFRYVLDGMNAERILIGAECIGDAKWFIERASAYARERQVFGRP
                     IGQNQGVQFPIARAYMAMRAAELMVERAAALYEEGAPCGPEANMAKQLAAEASWQAGD
                     MCLQTHGGFGFAEEYDVERKFRETRLYQVAPVSTNLVLCYLAEHVLELPRSY"
     gene            complement(2717..3541)
                     /locus_tag="VI319_15215"
     CDS             complement(2717..3541)
                     /locus_tag="VI319_15215"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEY6865243.1"
                     /translation="MPLVAMTREMGSMGMDVARIVESELGVPLVYHEIIDHLADKMRL
                     RKSHVIRLLGGKASLLERLTADKTSLSIFTADEVLQTASKGAVLRGWGAAHLLRPIKH
                     AICVRVCAPLELRVQRMMERLDTDDRGAVETEVKNSDEAQGAIVRRHFGVDWQHPELY
                     DLALNTERMSVEQCAEKVIGLARDPRFAETEASRADLDDLVQVAKVRAALRTDPATRE
                     LSVAIRVRDGAVTLEGVVDSAEQAKNAERVAARAPGVRSVENLLRAAEEVGRTVRE"
     gene            3780..4742
                     /locus_tag="VI319_15220"
     CDS             3780..4742
                     /locus_tag="VI319_15220"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865244.1"
                     /translation="MKPTKYLVLAAALSLAASVVCAQDWPQHPVKVVVPFPAGGNTDV
                     QARIVSGRLSNEYGQQFLVENRVGAAGAIAAEYVARAPADGYTLFFSSSPQISIVPLV
                     QKVTYDPIKDFAPISIVGTNPFVLGVQESLPVEDLQGFVKYVKEHQGLLNFASGGTGS
                     IGHLSAVLFLQRAGLVMTHIPYKGGAPAVQDLVAGQVQMYFGNASELLQHAKSGKIKL
                     LAVSSEKRAPQLPDVPAVAEYYKGFRTITWNGFLAPTGTPKAVIDSLAREVAKIVHEP
                     ETREKLEKIGVDPLGNTPAQFAAFIRAEAPVWHQAVKAADIKPE"
     gene            complement(4747..6069)
                     /locus_tag="VI319_15225"
     CDS             complement(4747..6069)
                     /locus_tag="VI319_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY6865245.1"
                     /translation="MTPTHQGLLVLVVTLVMLLSGVPVAFGLGALSIAFLVLFQGFDS
                     LRVVAETFYAGLNDFTLVSIPMFVMMGAAIGSSPAGKDLYEALDRWLYRLPGGLVISN
                     LGACAIFAALTGSSPACCAAIGKMGIPEMRRRGYPDDVATGSICAGGTLGILIPPSIT
                     FILYGIATETSIGRLFLAGVMPGLLLTGLFMLWTLFIIWKRGFRSHAIDFRYSWKQKF
                     GSIPKIAPFIAIIVGVMYVLYGGVATPSEAAGVGAALCVVCAVVIYRMWDAKSWWQIL
                     RDTTRESVMILTIIAAAVLFGYMLTSLYLTQTLAQTIADMHVNRWVLMGMINVFLLVC
                     GFFIPPAAIILMTSPILLPIITAAGVDPVWFGVILTINMEVGLIHPPVGLNIYIVQAI
                     APDVPVTRVMWGTIPYVLCMFLAIVILSFFPEIATWLPDTLMGPVLHK"
     gene            complement(6066..>6607)
                     /locus_tag="VI319_15230"
     CDS             complement(6066..>6607)
                     /locus_tag="VI319_15230"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY6865246.1"
                     /translation="PRGRQPMQGYVRVMRAISQLCGFIAAGMIAAGVIVVCEMVVLRY
                     VFGQSTIWQTDFTTYCLIAATFVGSPYVLMTRGHVNVDILPHYLGPKARRRLAYASSA
                     VTILFAVMLAVLTGNFWMEAWEQRWVSDTMWRARLWIPYSSMPIGLTLLTLQALAELV
                     QLVKGEATPFGMPDAEHME"
BASE COUNT         1076 a         2274 c         2291 g          966 t
ORIGIN      
        1 ggcgcgctcc tcgccccgta cacggccgcg ctccagtggc tctgcgcccg cgccggccca
       61 ctgacccccg ccgcgctcga ccacgcgctc gcccacccgc cgcccgacac ccgcgagctc
      121 cgcgcctccg acacggcgtc gagccaagct gacgcgccga cggccggacg ccgcgatgag
      181 cgatgagcac acgagaccga gccgaccacc gacccgacaa cgcggcaacg cgagcgcggc
      241 cggcacgcgg tcgcgcacaa cctcaggcga tggcgcccga ggtgcgcagc tccgcgacgc
      301 gttcgcccag gccgagctcg gcgaggatct cgtcggtgtg ctcgccgatc tcgggcggcg
      361 cgctcgcgat cgacgccggc gtgcgcgaca gccccaccgc ctgattcacg atgcggaact
      421 cgccgagctt ccggctgctc accttcgccg ccgcgtggat gtgctgcacc tgcgggtcgg
      481 cgaacatctt gtcgatcgag tagatgggac cgcaggggac gttcccccgg ctgaggatct
      541 cgatccactc ggcgctcgtg cgcgccgcgg tcgccttgtt gatctcggca ttcagcgcga
      601 cgcggttcct cgagcggttc gcctcgccct tgaagtcgtc acgctcgagc aggtccttgc
      661 gctccaggcc ctcgcacacg cgccgccaca tggtctcgcc ggtcgccgcg atgttgatgt
      721 agccatcctt cgtcttgtac gccgacgtcg gcatgctggt cggatggtcg ttgccggcct
      781 gccccggcac gtcgcccttc atcaggtagc gcgccgcctg gaagtcgagc agcgcgatct
      841 gcgacatgag cagcgacgac tgcacccact gccccttgcc cgaggcctcg cgctcgagga
      901 gcgcggtgag aacgccgagc gcggcgaaca gtcccgcgga gatgtcggcg atcgcggtgc
      961 cggcgcgcac cggcccctgg ccgggcatcc cggtgatcga catgaagccg cccatgccct
     1021 gcgcgatctg gtcgaacccg gggcggtcgc ggtacgggcc gtcctgcccg aagcccgaga
     1081 tgctcgcgag cacgatcttc ggattgactc tcgcgagcga ttcgtagccg atgccgagac
     1141 ggtccttcac gtcgggacgg tagttctcga ccacgacgtc cgagcgcttc accagctcca
     1201 tgaagatgcc gacgccctcg cgctccttca ggttgagcgt catcgagcgc ttgttgcggt
     1261 gcaggttctg gaagtccggc ccgtggcggg gcccccccat cgtgtcggcg tcgaccgagg
     1321 gcggcgtctc gatcttgatc acgtcggccc cgaagtcggc gagctggcgc acgcaggtcg
     1381 gccccgcgcg cacgcgcgac aggtcgagga cgcggaagcg cgcgagcgcg gtcgatgcgg
     1441 gaacgtgggg catgggccag cggatgcttg tcagcgggcc cttataataa ccgctccagg
     1501 gcgatgaccg ggggaagccg agatgtccgc gcgcgatcag gacctgcggg aaatccgcga
     1561 cgcggtcgcg aagctgtgcg cgagcttccc gatggagtac tggcgcgcgc tcgaccgcga
     1621 gcggaagtat ccgaccgagt tcgtgcgcgc gctgaccggg gccggatggc tctcgtgcct
     1681 gatcccggag agctacggcg gcgcggggct cggcctgcgc gccgcggcgg ccgtgctcga
     1741 ggaggtgcag cgcagcggcg ccaacggcgc cgcgtgccac gcccagatgt acacgatggg
     1801 cacggtgctg cgccactgca gcgaggcgca gaagcgggcg atcctgccga agctcgcgag
     1861 cggcgagctg cggctgcagg cgttcggcgt gaccgagccg accgcgggca ccgacacctc
     1921 gtcgatccgc accgccgccg tgaggaaggg cgaccgctgg gtcgtgaacg gccagaaggt
     1981 ctggacttcg cgcgccgagc actccgatct catgctgctg ctcgcgcgca ccacgccgcg
     2041 cgagcaggtc gcgaagcgaa ccgacggcct gtcgatcttc ctggtcgaca tgaaggctgc
     2101 gctcgggaaa ggtctctcga tccggccgat ccgcaccatg atcaaccacg cgactaccga
     2161 ggtgttcttc gacaacctcg agatccctgc ggagaacctg gtcggcgagg agggcaaggg
     2221 cttccgctac gtcctcgacg gcatgaacgc cgagcgcatc ctgatcggcg ccgagtgcat
     2281 cggcgacgcg aagtggttca tcgagcgcgc gagcgcgtac gcgcgcgagc gccaggtgtt
     2341 cgggcgcccg atcgggcaga accagggcgt gcagttcccc atcgcgcgcg cctacatggc
     2401 gatgcgcgcg gccgagctga tggtcgagcg cgccgcggct ctctacgagg aaggggcgcc
     2461 ttgcggcccg gaagcgaaca tggccaagca gctcgccgcg gaggcgtcgt ggcaggcggg
     2521 cgacatgtgc ctgcagacgc acggcgggtt cggcttcgcc gaggagtacg acgtcgagcg
     2581 caagttccgc gagacccggc tgtaccaggt cgcgccggtg tcgaccaacc tggtgctgtg
     2641 ctacctggcc gagcacgtcc tcgagctgcc gcgctcgtat tgagcgcgcg ccgattttgg
     2701 cgtaccgggc gcgcgcctac tcgcgcacgg tccggccgac ttcctccgcg gcgcgcagca
     2761 ggttctcgac cgagcgcacg cccggcgcgc gggcggcgac gcgctccgcg ttcttcgcct
     2821 gctccgcgga gtcgaccacg ccctcgaggg tcacggcgcc gtcccgcacc cggatggcca
     2881 ccgacagctc gcgcgtggcg ggatcggtgc gcagcgccgc gcgcaccttc gccacctgca
     2941 ccaggtcgtc gaggtcggcg cgcgaggcct cggtctcggc gaagcgcgga tcgcgcgcga
     3001 ggccgatcac cttctcggcg cactgctcga cgctcatgcg ctcggtgttg agcgcgaggt
     3061 cgtagagctc cgggtgctgc cagtcgacgc cgaaatggcg gcgcacgatc gcgccctggg
     3121 cctcgtcgct gttcttgacc tcggtctcga ccgccccgcg gtcgtcggtg tcgagccgct
     3181 ccatcatgcg ctggacgcgc agctcgagag gcgcgcacac acgcacgcag atcgcgtgct
     3241 tgatcggccg cagcaggtgc gccgcgcccc agccgcgcag taccgcgccc ttgctcgcgg
     3301 tctgcaacac ttcgtcggcg gtgaagatcg acaggctcgt cttgtcggcg gtgagccgct
     3361 cgagcaggct cgccttgccg cccaggagcc ggatcacgtg gctctttcgc agccgcatct
     3421 tgtcggcgag gtgatcgatg atctcgtggt agacgagcgg gacgcccagc tcggactcga
     3481 cgatccgggc gacgtccatg cccatcgatc ccatctctct cgtcatggca accagcggca
     3541 tggctcctcc cttggagtac ggcttgggct gcgatcggaa ggcggcagtg taggtgcgtc
     3601 cgcgcgaaaa ggaaagcccc cgggcccgct tgcggcaaac ccaatgacga cgacataagg
     3661 ggcttccgga cgcgcgtccc gaacgtccgt cccggcgcca aattgttccg cgtgcagggc
     3721 gcccgcttcg gtagaattta gacgcagtcc ggatcgaccc ggacgcggga ggaggagaca
     3781 tgaaacccac aaaatatctt gtgctcgcgg cggcgctttc gctggccgcg tcggtcgtct
     3841 gcgcccagga ctggccgcag catccggtga aggtcgtggt gccgttcccc gcgggcggaa
     3901 acaccgacgt gcaggcgcgc atcgtctcgg ggcgcctgtc gaacgagtac gggcagcagt
     3961 tcctcgtcga gaatcgcgtc ggcgccgccg gcgcgatcgc ggccgagtac gtggcgcgcg
     4021 ccccggcgga cggctacacg ctgttcttct cgtcctcgcc ccagatctcg atcgtgccgc
     4081 tggtgcagaa ggtgacctac gacccgatca aggacttcgc ccccatcagc atcgtcggca
     4141 cgaacccgtt cgttctgggc gtgcaggagt cgctcccggt ggaggatctt caagggttcg
     4201 tgaagtacgt gaaggagcac cagggcctgc tcaacttcgc ctcgggcggc acgggctcga
     4261 tcgggcacct gtcggcggtc ctcttcctgc agcgcgcggg cctcgtgatg acgcacatcc
     4321 cgtacaaggg cggcgcgccg gcggtgcagg acctggtcgc cggccaggtg cagatgtact
     4381 tcggcaacgc ctcggagctg ctgcagcacg cgaagagcgg caagatcaag ctgctcgccg
     4441 tgtcgagcga aaagcgcgcg ccccagctgc ccgacgtgcc ggcggtcgcc gagtactaca
     4501 aggggttccg cacgatcacc tggaacggct tcctggcccc gacgggcacg cccaaggcgg
     4561 tgatcgactc gctcgcgcgc gaggtggcga agatcgtgca cgagcccgag acgcgggaga
     4621 agctcgagaa gatcggcgtc gatccgctcg gcaacacgcc ggcgcagttc gccgcgttca
     4681 tccgggccga ggcgccggtg tggcaccagg cggtgaaggc cgccgacatc aagccggagt
     4741 agcgctctac ttgtgcagca ccggccccat cagggtgtcg ggcagccagg tcgcgatctc
     4801 ggggaagaag ctcaggatca cgatcgcgag gaacatgcag agcacatagg ggatcgtccc
     4861 ccacatgacg cgggtcacgg gcacgtcggg ggcgatcgcc tgcacgatgt agatgttcag
     4921 cccgacgggc gggtggatca gcccgacctc catgttgatc gtgaggatca cgccgaacca
     4981 caccgggtcg acgcccgccg cggtgatgat cggcagcagg atcgggctcg tcatcaggat
     5041 gatcgccgcg ggcgggatga agaacccgca cacgagcagg aacacgttga tcatgcccat
     5101 cagcacccag cggttgacgt gcatgtcggc gatcgtctgg gcgagcgtct gcgtcaggta
     5161 cagcgacgtc agcatgtagc cgaacagcac ggccgcagcg atgatggtca ggatcatcac
     5221 cgactcgcgc gtcgtgtcgc gcaggatctg ccaccagctc ttcgcgtccc acatccggta
     5281 gatcacgacc gcgcagacca cgcacagcgc ggcgccgacg ccggcggcct cggacggcgt
     5341 cgccacgcct ccatagagca cgtacatcac gccgacgatg atggcgatga acggggcgat
     5401 cttcgggatc gagccgaact tctgcttcca gctatagcgg aagtcgatcg cgtgcgagcg
     5461 gaagccgcgc ttccagatga tgaacagcgt ccacagcatg aacaggcccg tgagcagcag
     5521 cccgggcatg acgccggcga ggaacaggcg cccgatcgag gtctcggtgg cgatgccgta
     5581 gaggatgaac gtgatcgacg gcgggatgag gatgccgagc gtgccgccgg cgcagatcga
     5641 gccggtggcg acgtcgtcgg ggtagccgcg gcggcgcatc tccgggatgc ccatcttgcc
     5701 gatcgccgcg cagcacgcgg gcgaggagcc cgtcagcgcg gcgaagatcg cgcacgcgcc
     5761 gaggttcgag atgaccaggc cgcccgggag ccggtagagc cagcggtcga gcgcctcgta
     5821 gagatctttc cccgcgggcg aggagccgat cgccgcgccc atcatcacga acatcgggat
     5881 cgacaccagc gtgaagtcgt tcagccccgc gtagaaggtc tcggcgacca cgcgcagcga
     5941 atcgaagccc tggaacagga cgaggaaggc gatcgagagc gcgcccaggc cgaacgcgac
     6001 cgggactccc gagagcagca tcacgagcgt cacgacgagg accagcagtc cctggtgggt
     6061 gggggtcatt ccatgtgctc cgcgtcgggc atgccgaagg gcgtcgcctc gcccttcacg
     6121 agctggacca gctcggcgag cgcctgcagc gtgagcagcg tgaggccgat cggcatcgac
     6181 gagtacggga tccacaggcg cgcgcgccac atcgtgtcgg agacccagcg ctgctcccag
     6241 gcctccatcc agaaattccc ggtcagcacg gcgagcatga ccgcgaacag gatggtgacc
     6301 gcgctcgagg cgtacgccag ccgccggcgc gccttcggcc ccaggtagtg cggcaggatg
     6361 tcgacgttga cgtggccgcg cgtcatcagc acgtaggggc tgccgacgaa cgtcgcggcg
     6421 atcaggcagt aggtagtgaa gtcagtctgc cagatcgtgc tctggccgaa gacgtagcgc
     6481 agcacgacca tctcgcacac cacgatcacc ccggccgcga tcatgcccgc ggcgatgaac
     6541 ccgcacagct gcgagatcgc gcgcatgacg cgcacgtagc cttgcatcgg ctgcctccct
     6601 cgcggat
//
LOCUS       DATDMW010000363         5908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1723745, whole genome shotgun sequence.
ACCESSION   DATDMW010000363 DATDMW010000000
VERSION     DATDMW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723745"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VI319_15235"
     CDS             complement(<1..280)
                     /locus_tag="VI319_15235"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY6865247.1"
                     /translation="MTCCHYYAITDDHESAFSVDIASISFGPGVLKEVGDHARALGMK
                     RVAFFTDKRVGNLECVADSLSSLRTAGCDVSIYDEVSVEPTDASFRSAA"
     gene            complement(288..1628)
                     /locus_tag="VI319_15240"
     CDS             complement(288..1628)
                     /locus_tag="VI319_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6865248.1"
                     /translation="MTTLNHRAKVESLLAGLRSGASVGLAKKTSNLFRDRQRSAKPKV
                     DLSGFDEVVGVDATAGTVEAEGMTTYARLVDETLAHGAMPCVVPQLKSITLGGAAAGV
                     GIEASSFKYGLAHETLAAIDVLLADGTVAHCTPDNEHRDLFFGFPNSYGTLGYALTLT
                     TKTVPVKPYVAIQHLRHHDPRRYFEHLADLCSARNADFVDGTVFSGKEMYLTVGRFSD
                     HAARTSDYTYEHIYYRSIREKESDCLTTRDFIWRWDTDWFWCSKNLYVQNPVVRRIVG
                     RKRLNSTTYTKVMRWNSKWGITRALTRLSGRTPESVIQDVDIPIERCAEFLEFFIKEI
                     GITPVWICPIRAWDANNDFVLYRYDPKTTFVNFGFWDVVRSAEEHPAGWYNRMIERKV
                     RELGGIKSLYSDSYYPEDEFWKIYNRPAYEALKKKYDPEGRFKDLYQKCVLRSG"
     gene            complement(1654..2076)
                     /locus_tag="VI319_15245"
     CDS             complement(1654..2076)
                     /locus_tag="VI319_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865249.1"
                     /translation="MRSIFEMLAAHGTSPYAAVQFAVCAGGLVGCVGWALASPRWAYV
                     VGAAAAAFLLVHGFRYFVVRVSGFAGIMSVSEALWWPIYSTWTLAVRLLAQGRIAAGL
                     GLVLEEWLVPLLQLAVLAAAVRTLVTRSPGDVPQAAGA"
     gene            complement(2135..3436)
                     /locus_tag="VI319_15250"
     CDS             complement(2135..3436)
                     /locus_tag="VI319_15250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY6865250.1"
                     /translation="MDTRVSSVESRHDQVGRAGPDTSRGGAVQKIMRSIGAATSVCFR
                     VVFPDGASYQNHERAPDVTVLFRNRRALRRVVMFGHVGFLEGYFEGDVDIEGNLALAI
                     RAGMDAGFDRAPGLLVRIRNWWHEFRFSNASIEQARDNACFHYSLGRDFYKHWLDEYG
                     LMYTCAWWKEGTKTLEEAQKNKMDLVCRKVLLKPGETFADIGSGFGGLLFHAHEHYGA
                     LGTGINASDIQVKETREEIKKRGLEGKVEVLQGKIECLERDFRQPPRQFDKLLSIGTL
                     EHAGRDQMMEVIQAHADFMKPGALGVIHFIGHVGVRPTEFWIRRYIFPGGWIPSLAQA
                     IDCCEKAGLEVVDVENLRRNYALTLDAWAERFDRNWDKIRALDPNKFDERFRRIWRTY
                     LWGCAEMFRSPNGRTHLFQIVVSKGNLTATNYPMSRKFLYE"
     gene            3496..>5908
                     /locus_tag="VI319_15255"
     CDS             3496..>5908
                     /locus_tag="VI319_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEY6865251.1"
                     /translation="MRAASRFDPARVHALAADLAAAVSGEVRFDAGSRALYAADASNY
                     RQVPIGVVVPRSAEDVVEAVRVCRAHGAPILARGGGTSQNGQCVNVAVVIDSSKYLNR
                     ILSIDAQARTAVVEPGVVCDALRAAAERHGLTFGPDPATHSRCTLGGMIGNNSCGAHS
                     VMAGKTDGNIEQLEVLTCDGARFRAGATPPAELAAIVAAGGRRGEIYAALARLAERYG
                     ELIRARFPKIKRRVSGYNLDQLLPENGFHVGRALVGSEGTCALVLAATAKLVRSPPQR
                     VLLVLGYPDIYSAAAAVPAIMPHGPIACEGLDHFLIGGLERRGLRAADVALLPAGRAW
                     LMIEFGGDSRDEAVEKAHRVSAGETGRLLLVEPEMQTRVWTIRETAASHTALSIDPGV
                     PDPAVGWEDAAVDPLRLPEYLRAFQALVDRHGYRTSLYGHFGDGCVHARITFDLRTHE
                     GVREWRTFLREAAELVVAHGGSLSGEHGDGQAKAELLPIQYGPELMQAFREFKAIWDP
                     ENRMNPGKLVDPYRVDENLRLGPDYRPVSVSTRMAFLSPEGDGFARAMERCIGMGRCR
                     SAAGGTMCPSYRATLEERHSTRGRAHVLAEMLRGDVIADGWKSDAVREALDLCLSCKG
                     CASDCPTHTDVAAYKAEFLSHYYEGAGRRRPRQAHAMGRIGDWAPFAGAYPGVSNFLT
                     RGPLVGAAAKRLAGIASERELPRFARMPFRAQFARNARAPRAHGEAVGREVLLWVDSF
                     TNFFEPRIGHAAVEVLEAAGCRVSIGAKRLCCGRPLYDFGMLDAARASRGADDDAVGL
                     PRGDRL"
BASE COUNT          883 a         1996 c         2110 g          919 t
ORIGIN      
        1 gggcggcaga ccggaacgag gcgtccgtcg gctcgacgga aacttcgtcg tagatggaga
       61 cgtcgcagcc ggcggtgcgg agagacgaga gtgagtcagc cacgcattcg agatttccca
      121 cgcgcttgtc cgtaaagaac gcgacgcgct tcatcccgag cgcgcgcgcg tggtcgccca
      181 cttccttcag gacgcccggg ccgaacgaga tgctcgcgat gtcgaccgaa aaggccgact
      241 cgtggtcgtc ggtgatggcg tagtagtggc agcaggtcat gaggacgtca tcctgagcga
      301 agcacgcact tctggtacag gtccttgaac ctcccctccg gatcgtactt cttcttcagc
      361 gcctcgtacg ccggccggtt gtagatcttc cagaactcgt cctccggata gtaggagtcc
      421 gaatacagcg attttattcc cccgagctcg cgcaccttgc gctcgatcat gcggttgtac
      481 cagccggcgg ggtgctcctc ggccgagcgg acgacgtccc agaacccgaa gttcacgaac
      541 gtcgtcttcg ggtcgtagcg gtagagcacg aagtcgttgt tcgcgtccca cgcccggatc
      601 gggcagatcc acaccggcgt gatgccgatc tccttgatga agaactcgag gaactcggcg
      661 cagcgctcga tcgggatgtc gacgtcctgg atcaccgact cgggcgtgcg gcccgagagg
      721 cgcgtcagcg cgcgcgtgat gccccacttg ctgttccagc gcatgacctt ggtgtaggtc
      781 gtcgagttca gtcgcttgcg gccgacgatc ctgcgcacga ccgggttctg cacgtagagg
      841 ttcttcgagc accagaacca gtcggtgtcc cagcgccaga tgaagtcgcg cgtggtcagg
      901 cagtcgctct ccttctcgcg gatcgagcgg tagtagatgt gctcgtaggt gtagtcgctc
      961 gtgcgcgccg cgtggtcgga gaagcgcccg acggtgaggt acatctcctt gccggaaaac
     1021 acggtgccgt cgacgaagtc ggcgttccgg gcggagcaca gatcggccag gtgctcgaag
     1081 tagcgccggg gatcgtgatg ccgcaggtgc tggatcgcga cgtagggctt caccggcacg
     1141 gtcttcgtcg tgagcgtgag cgcgtagccg agcgtgccgt aggagttcgg gaagccgaag
     1201 aacaggtcgc ggtgctcgtt gtcgggcgtg cagtgcgcga ccgtgccgtc ggcgagcagg
     1261 acgtcgatcg ccgcgagcgt ctcgtgcgcg aggccgtact tgaacgacga ggcctcgatg
     1321 ccgacgccgg ccgcggcgcc cccgagcgtg atcgacttca gctgcggcac gacgcagggc
     1381 atcgcgccgt gcgcgagcgt ctcgtcgacg aggcgcgcgt acgtcgtcat gccctcggcc
     1441 tcgaccgtgc cggcggtcgc gtcgacgccg acgacttcgt cgaagccgga gaggtcgacc
     1501 ttgggcttcg ccgagcgctg cctgtcgcgg aagaggttgg aggttttctt cgcgagcccg
     1561 acgctcgctc ccgagcgcag gccggcgagc aggctctcga ccttggcgcg atggttgagc
     1621 gtggtcatcc cagcggcggc gtctggtcgc cgatcaggcg cccgccgcct gcgggacgtc
     1681 gccgggcgag cgcgtcacca gcgtccgcac ggcggcggcg agcacggcga gctggagcag
     1741 cggcacgagc cactcctcga gcacgagccc gaggccggcc gcgatccggc cttgcgcgag
     1801 caggcgcacg gccagcgtcc acgtggaata gatcggccac cacagcgcct cggagaccga
     1861 catgatcccg gcaaacccgg agacgcggac gacgaagtag cggaagccat ggacgagcag
     1921 gaaagcggcg gcggcggccc cgaccacgta cgcccagcgc ggcgaggcga gcgcccagcc
     1981 gacgcagccg acgagaccgc ccgcgcagac cgcgaattga accgcggcgt acggcgaggt
     2041 cccgtgcgcc gccagcatct cgaaaatgga gcgcatgata gcgatcgacc acgacgccga
     2101 ggccacgagc aacgccgttg tcttcaacgg acgcctactc gtacaggaac ttgcggctca
     2161 tcgggtagtt cgtcgcggtg aggttgccct tgctcacgac gatctggaac agatgcgtgc
     2221 gtccgttcgg ggagcggaac atctcggcgc agccccagag atacgtgcgc cagatgcggc
     2281 ggaagcgctc gtcgaacttg ttcggatcga gcgcgcggat cttgtcccag ttgcggtcga
     2341 agcgctcggc ccaggcgtcg agcgtgagcg cgtagttgcg gcgcaggttc tcgacgtcga
     2401 cgacctcgag gcccgccttc tcgcagcagt cgatcgcctg ggcgaggctc gggatccagc
     2461 cgccggggaa gatgtacctg cggatccaga actcggtcgg acgcacgccg acgtgcccga
     2521 tgaagtggat gaccccgagc gcgcccggct tcatgaagtc ggcgtgcgcc tggatcacct
     2581 ccatcatctg gtcgcggccc gcgtgctcga gcgtgccgat cgagagcagc ttgtcgaact
     2641 ggcgcggcgg ctggcggaag tcgcgctcga ggcactcgat cttgccctgc aggacctcga
     2701 ccttgccctc gaggccgcgc ttcttgatct cctcgcgcgt ctccttcacc tggatgtcgc
     2761 tcgcgttgat cccggtgccc aaagcgccgt aatgctcgtg cgcgtggaac agcaggccgc
     2821 cgaagccgct gccgatgtcg gcgaaggtct cgcccggctt gagcagcacc ttgcggcaga
     2881 cgaggtccat cttgttcttc tgcgcctcct cgagcgtctt cgtgccttcc ttccaccagg
     2941 cgcaggtgta catcagtccg tactcgtcga gccagtgctt gtagaagtcg cgccccaggc
     3001 tgtagtggaa gcaggcgttg tccctggcct gctcgatcga cgcgttgctg aatcggaact
     3061 cgtgccacca gttgcggatg cgcaccagca gcccgggcgc gcggtcgaag cccgcgtcca
     3121 tgccggcgcg gatggcgagc gcgagattgc cctcgatgtc gacgtcgccc tcgaaatagc
     3181 cctcgaggaa gccgacgtgc ccgaacatga cgacgcgccg cagcgcgcgg cggttgcgga
     3241 acagcacggt gacgtcgggg gcgcgctcgt ggttctggta cgacgcaccg tcgggaaaaa
     3301 cgacgcggaa gcacaccgag gtcgccgcgc cgatcgagcg catgatcttc tgcacggcgc
     3361 cgccgcggct cgtgtcgggg ccggcgcggc cgacctggtc gtgacgcgat tcgacggagg
     3421 agacacgcgt atccattgcc cgaagtatat gcggcttttg ctacactcgt cgctggcgat
     3481 cccgaccata agaccttgcg cgccgcctcc cggttcgacc ccgcccgagt ccacgccctc
     3541 gccgccgacc tcgcggcggc ggtgagcggc gaggtccggt tcgacgcggg cagccgggcg
     3601 ctctacgccg cggacgcctc caactaccgc caggtgccga tcggggtggt cgtgccgcgc
     3661 agcgcggagg acgtggtcga ggcggtgcgc gtgtgccgcg cccacggcgc cccgatcctc
     3721 gcgcgcggcg gcggcacgtc gcagaacggc cagtgcgtga acgtcgcggt cgtgatcgat
     3781 tcctcgaagt acctgaaccg gatcctgtcg atcgacgcgc aggcgcgcac ggcggtggtc
     3841 gagccgggcg tcgtctgcga cgcgctgcgc gcggcggccg aacgccacgg cctcacgttc
     3901 ggccccgatc ccgcgaccca cagccgctgc acgctcggcg ggatgatcgg caacaactcg
     3961 tgcggcgcgc actccgtgat ggcgggcaag accgacggca atatcgagca gctcgaggtg
     4021 ctcacctgcg acggcgcgcg ctttcgcgcc ggggcgacgc cgccggccga gctcgcggcg
     4081 atcgtcgcgg cgggcggccg gcgcggcgag atctacgccg cgctcgcgcg cctcgccgag
     4141 cgctacggcg agctgatccg cgcgcgcttc ccgaagatca agcgccgggt ctcgggctac
     4201 aacctcgacc agctgctgcc cgagaacggc ttccacgtcg gccgcgcgct ggtcggctcg
     4261 gagggcacgt gcgcgctggt gctggcggcg acggcgaagc tggtgcgcag cccgccgcag
     4321 cgggtgctgc tggtgctggg ctaccccgat atttattccg cggcggcggc cgtgcccgcg
     4381 atcatgccgc acgggccgat cgcgtgcgaa gggctcgacc atttcctgat cggcgggctc
     4441 gagcgccgcg ggctgcgcgc cgccgacgtc gcgctgctgc ccgcgggccg cgcgtggctg
     4501 atgatcgagt tcggcggcga ctcgcgcgac gaggcggtgg agaaggcgca ccgggtgagc
     4561 gccggcgaga ccggccggct gctgctggtc gagcccgaga tgcagacgcg cgtgtggacg
     4621 atccgcgaaa ccgccgcttc ccacaccgcg ctctcgatcg atccgggggt gccggatccc
     4681 gcggtcggct gggaggacgc ggcggtcgat ccgctgcgcc tgcccgagta cctgcgcgcg
     4741 ttccaggcgc tcgtcgaccg ccacggctat cgcacctcgc tctacggcca tttcggcgac
     4801 ggctgcgtgc acgcgcgcat cacgttcgac ttgcgcacgc acgagggtgt ccgcgaatgg
     4861 cgcacgttcc tgcgcgaggc cgccgagctg gtcgtcgcgc acggcggctc gctctcgggc
     4921 gagcacggcg acggccaggc gaaggcggag ctgctgccga ttcagtacgg gcccgagctg
     4981 atgcaggcct tccgcgagtt caaggcgatc tgggacccgg agaaccggat gaatccgggc
     5041 aagctcgtcg atccctaccg cgtcgacgag aacctgaggc tcgggcccga ctaccggccg
     5101 gtgagcgttt cgacgcgcat ggctttcctc tcgcccgagg gcgacggctt cgcgcgcgcg
     5161 atggagcgct gcatcggcat gggccgctgc cgctcggcgg cgggcggcac gatgtgcccg
     5221 agctaccgcg cgacgctcga ggagcggcac tcgacgcgcg ggcgcgcgca cgtgctcgcc
     5281 gagatgctgc gcggcgacgt gatcgcggac ggctggaaga gcgacgcggt gcgcgaggcg
     5341 ctcgacctgt gcctgtcgtg caagggctgc gcgagcgatt gcccgacgca tacggacgtc
     5401 gccgcgtaca aggccgagtt cctgtcgcat tactacgaag gcgccggccg gcggcggccg
     5461 cgccaggcgc acgcgatggg acgcatcggc gactgggcgc cgttcgccgg cgcttatccc
     5521 ggcgtctcga atttcctcac gcgagggccg ctcgtgggtg ccgcggcgaa gcggctcgcc
     5581 ggcatcgcgt ccgagcgcga gctgccgcgc ttcgcgcgca tgccgtttcg cgcgcaattc
     5641 gcgcgaaatg cgcgagcgcc gcgcgcgcac ggggaagccg tcgggcgcga ggtcctgctc
     5701 tgggtcgata gcttcacgaa cttcttcgag ccgcgcatcg gccacgcggc ggtcgaggtg
     5761 ctcgaggccg ccgggtgccg cgtgtcgatc ggcgcgaagc ggctgtgctg cggccggccg
     5821 ctctatgact tcggcatgct cgacgccgcg cgcgcgtcgc gcggcgcaga cgacgatgct
     5881 gtcggacttc ctcgaggcga tcggctgg
//
LOCUS       DATDMW010000364         8758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1474418, whole genome shotgun sequence.
ACCESSION   DATDMW010000364 DATDMW010000000
VERSION     DATDMW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474418"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VI319_15260"
     CDS             complement(<1..538)
                     /locus_tag="VI319_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY6865252.1"
                     /translation="MKPPRFSYYDPETVPEVVGLLRKLENSRVLAGGQSLMPMLNMRL
                     ALPDHVIDLNGVTELSYLREKSDALEIGAMTRQREIEFSDKVRARCPLMHEAILQVGH
                     RQTRNRGTLGGSLCQLDPSAELVTVAAALDATVTVAGPKGAREIAFADFPKTYMTPAI
                     EADELVTGAKFPLWPESHG"
     gene            complement(535..2697)
                     /locus_tag="VI319_15265"
     CDS             complement(535..2697)
                     /locus_tag="VI319_15265"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6865253.1"
                     /translation="MTNVIGRRVARLEDAPMLTGRGRYAADVSFPRELHMRVVRSSHA
                     HARIVSIDTASARQLPGCVAAWTSADVAAIPPIEFRPTRVKGLEPYRQPVLARGRVRY
                     VGEPVAAVFAEDPYAAEDAADLVTMEMEPLPILLSADAAPGEFDAGLSTEAAVIRKGY
                     GDVDAAFAAAHAVIELDLSIGRHSGVPLECRGALARHDAARDVLELHGATKRPHANRD
                     LLARMLGRTPASIHLYEGHVGGGFGVRGEIYPEDLLVCAAALALRRPVKWIEDRRENL
                     MACNHSRQQRHRVRAAVDAQGGILAIDDEFFHDQGAYVRTHGARVADMTAGMLPGPYR
                     VPAYRAVGHYRLTNKTPAATYRAPGRYEATFVCERLMDAIAARLGVDRVEVRRRNLVA
                     KAEMPYTRDMDVLDTELVLDSGDYGGLLDKALARIEWPALQRELAKRRAAGEAVGAGI
                     ACFVEKSGLGPVDFARVAAAEDGGFEVITGAASLGQGVETVMAQIAAEALGVDYARVR
                     VTHGRTDRIDRGYGSHASRTTVMTGSAVHVAASALRAKMDAAGGREPGLSATGTFESA
                     HMTYPYGVHIAVVRVDRETGGAHVERYLAAFDVGRAINPMLVEGQIAGGVVQGLGGAL
                     LEEFVYDAEGSPLAVTFADYTMATACEAPRIDVLLAEDAPSPLNPLGVKGAGEGGINP
                     VGAVIASAIDDALGRPGAVTRLPVTPQRLRAMLERTAA"
     gene            complement(2694..3737)
                     /gene="pyrC"
                     /locus_tag="VI319_15270"
     CDS             complement(2694..3737)
                     /gene="pyrC"
                     /locus_tag="VI319_15270"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY6865254.1"
                     /translation="MTDRLTIRKPDDWHLHVRDGAMLRAALPFTARHFGRAIIMPNLL
                     PPVCTVKEAEQYRERIAKALPRGAKFEPLMTSYMTHETSPDEIEAGFRAGVFTGVKIY
                     PANVTTNSAAGVSDYRRIRRVVERMEKIGMRLLIHGEVIDPDVDVFDREAVFIERELE
                     PLARDFPGLKIILEHVSSKIGVDFVRSKQGQVGGTITPYHLMLTRTDWLGWGNRPYMY
                     CAPVIKTAKDRAALRAAATSGEPYFFLGTDSAPHPVAKKLDVVGAAGVFNAPVAIESY
                     AQVFAEENALDRLEAFASLNGPRHYGLPPNTETITLERSAWTAPAEMKIDGPEERALV
                     HRGGETIPWRVVA"
     gene            complement(3734..4666)
                     /locus_tag="VI319_15275"
     CDS             complement(3734..4666)
                     /locus_tag="VI319_15275"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY6865255.1"
                     /translation="MKIGKLKLANPVMPASGTFSAEFGKIIDLNRLGALITKTVSRGF
                     RAGNPPPRVSEVDAGMINSIGLPTKGLEYFLEHQVPEYRRFTPPLVASVTSTEIEDFA
                     AMARDVSVPAVDAIELNISCPTREPGGGNFALNEEHTYKVVKRCRAATDKPLWAKLSP
                     NAGDISAVAEAAEKAGADAITVSNTILGLKINLDTFQSHIGQGYGGISGPGVKPIILR
                     MVHQCSRCVKIPIIGCGGIMRAEDVVEYLLAGATAVEVGYLNFRNPTGMIALIDGLEQ
                     WCAERKIERVASLTGAMKAHPPRDTYEAGMMGIS"
     gene            complement(4675..5541)
                     /locus_tag="VI319_15280"
     CDS             complement(4675..5541)
                     /locus_tag="VI319_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY6865256.1"
                     /translation="MTHPVHASAAVVVGQADTGHSPPQCPVEETLCPVVAHEWINAEY
                     KHLVVKASPKALAATPGQFFHLLCPSPDEGQLWFRRPQSVYRVARSSGRIEFLYKVVG
                     RGTKGLATLQPGDTLNMVGPLGVGFTLDPAWKNVVVLGRGVGLATMAPISQLAGERGV
                     RVTAILSARSEELAMAADLFESVGTVVKVLDTDGSSAVENVEKILRRLIAGKKADAFY
                     TCGSNRLMQLMKRLGKEHGIPGQMAMEQIMACGLGPCYICVRTFEVAGKLEMRRVCVE
                     GPVFDLQECLGW"
     gene            5679..7109
                     /locus_tag="VI319_15285"
     CDS             5679..7109
                     /locus_tag="VI319_15285"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY6865257.1"
                     /translation="MANPDLRDWLAQMDAAGELQKVSGANRQEEIGGIVDIYQRATGR
                     PALLFEDIPGYPKGYRVVANVLTGVKRIALTFGMPAESTEMDLVHYWKKYLEEQKTVA
                     PAVVKSGPVLENVLSGKEVNLAKIPTPLWHEDDGGPYIGTACMVIMKDPDSGWINYGA
                     YRVQAYDDPRIASVMCSKGKHGDLLMRRYHERGQRCPVAVVVGMHPLLFTMAGVELPY
                     GKNEYDAVGGMMGKPVEVILGPKTGLPIPANAEIAFEGSIAPDDLIKEGPFGEWTGYF
                     AGGEKQEPVIRIDTMMHRNDPILLGAVPAVPPNDDTFYRGAFRCAAVWRQLEAAGVLG
                     IRGVWAHEAGGSRMWLTVSIKQMFPGHAKQAGLIASQCHAGSYANKYVVVVDDDIDPA
                     NMNDVVWAMCTRVDAREDVEILRTSRSGPLDPTSYPDGIFAFNSRMVIDACRPWARRE
                     DFPKVSRSSKELDERIKAKWAHVLPR"
     gene            7085..8059
                     /locus_tag="VI319_15290"
     CDS             7085..8059
                     /locus_tag="VI319_15290"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865258.1"
                     /translation="MGARAAALIGLLLAGAACAQDYPSRPIHMVVAFPPGGIADFAAR
                     SVSTGLADALRVPVVVENRSGAGGSIGADFVAKSAPDGYTVLVTSISHTINPAFMKKL
                     PFDTRRDFAPVMMIADAPNLLVLHPSVPARTLAELIDWARAHPGALTYASSGVGTSTH
                     LCGELFAQMAKVDLVHVPYKGGGPAVADLLGGQVRMMFATLPTVLEHARSGRLRAIAT
                     TGAQRFAGAREFPTMAEAGAESGLSGYDVSAWTGMFAPAHTPGAAIDRLAQETAKILR
                     SPALEERLLAQGAEAAVKMPDEFGRFVDAELDKWKKLVESAHLRTEDK"
     gene            8056..>8758
                     /locus_tag="VI319_15295"
     CDS             8056..>8758
                     /locus_tag="VI319_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY6865259.1"
                     /translation="MKPNELTASDAARRIARRELTSEALVRACLERIEVREKTVRAWA
                     FIDPDAALAQARERDREPARGALHGVPIGVKDVLDTADMPTEMGSPIYAGWRPRADAA
                     CVAIARAAGAVVLGKTVTAELAGMEPGATRNPLDPSRTPGGSSSGSAAAVADFMVPLA
                     LGTQTGGSVLRPASVCGVIGFKPSFGRYNRSGLKMAAESLDTIGTMARKIDDIELLDA
                     ALTRAPARPPRAREGA"
BASE COUNT         1268 a         3160 c         3053 g         1277 t
ORIGIN      
        1 agccgtgcga ctcgggccac agcgggaatt tcgcgcccgt gaccagctcg tcggcctcga
       61 tcgcgggcgt catgtaggtc ttcgggaaat cggcgaaggc gatctcgcgc gcccccttcg
      121 gacccgcgac cgtcaccgtc gcgtcgagcg cggctgccac ggtcacgagc tccgccgacg
      181 gatcgagctg acacagcgag ccgccgagcg tgccgcggtt gcgcgtctgc cggtggccga
      241 cctggaggat ggcctcgtgc atcagcgggc agcgcgcgcg caccttgtcg gagaactcga
      301 tctcgcgctg gcgcgtcatc gcgccgatct cgagcgcgtc gcttttttcg cgcagatacg
      361 aaagctccgt cacgccgttg aggtcgatca cgtgatcggg cagcgcgagc cgcatgttca
      421 gcatcggcat cagcgactgg ccgccggcga ggacccggct gttctccagc ttgcgcagga
      481 ggccgaccac ctcgggaacc gtctcgggat cgtagtagga gaagcgcggc ggcttcatgc
      541 tgccgtcctc tcgagcatcg cccgcagccg ctgcggcgtg accggcagcc gcgtcacggc
      601 gcccggccgc ccgagcgcgt cgtcgatcgc cgacgcgatc accgcgccga cgggattgat
      661 gccgccctcg cccgcgcctt tcacgccgag cggattgagc gggctgggcg catcctcggc
      721 gagcagcacg tcgatccgcg gcgcctcgca cgccgtcgcc atggtgtagt ccgcgaaggt
      781 cacggcgagc ggcgagccct cggcgtcgta gacgaactcc tcgagcagcg cgccgcccag
      841 gccctgcacc acgccgcccg cgatctgccc ctcgaccagc atcgggttga tcgcgcgccc
      901 gacgtcgaag gcggcgaggt agcgctcgac gtgcgcgccg ccggtctccc ggtcgacgcg
      961 cacgaccgcg atgtgcacgc cgtagggata cgtcatgtgc gcgctttcga acgttcccgt
     1021 cgcggaaaga cccggctcgc gcccgcccgc cgcgtccatc ttcgcgcgca gcgcggaggc
     1081 ggcgacgtgc accgccgagc ccgtcatcac cgtcgtgcgc gacgcgtgcg agccgtagcc
     1141 gcggtcgatg cggtcggtgc gcccgtgcgt cacgcgcacg cgcgcgtagt cgacgccgag
     1201 cgcttccgcc gcgatctgcg ccatcacggt ctcgacgccc tgcccgagcg acgccgcgcc
     1261 ggtgatcacc tcgaagccgc cgtcctccgc ggccgcgacg cgcgcgaagt cgaccggccc
     1321 caggccgctc ttctcgacga agcacgcgat gccggcgccg accgcctcgc cggccgcgcg
     1381 gcgcttcgcg agctcgcgct gcagcgcggg ccactcgatg cgggcgagcg ccttgtcgag
     1441 caggcccccg tagtcgcccg agtcgagcac cagctcggtg tcgagcacgt ccatgtcgcg
     1501 cgtatagggc atctcggcct tcgcgaccag gttgcgccgc cgcacctcga cgcgatcgac
     1561 gccgagcctc gccgcgatcg cgtccatcag ccgctcgcac acgaaggtcg cctcgtagcg
     1621 ccccggcgcg cggtaggtgg cggcgggcgt cttgttcgtg agccggtagt ggccgacggc
     1681 gcggtacgcc ggcacgcggt aaggcccggg cagcatgccc gcggtcatgt cggcgacgcg
     1741 cgcgccgtgc gtgcgcacgt acgcgccctg gtcgtggaag aactcgtcgt cgatcgcgag
     1801 gatgccgccc tgcgcgtcga ccgcggcgcg cacgcggtgg cgctgctggc gcgagtggtt
     1861 gcaggccatc aggttctcgc gccggtcctc gatccacttc accgggcggc gcagcgcgag
     1921 cgcggccgcg cagaccagga ggtcctcggg atagatctcg ccgcgcacgc cgaagccgcc
     1981 gccgacgtgc ccctcgtaga gatggatcga cgcgggcgtg cgtcccagca tccgcgcgag
     2041 caggtcgcga ttcgcgtgcg ggcgcttggt cgcgccgtgc agctccagga cgtcgcgcgc
     2101 cgcgtcgtgg cgcgcgagcg cgccccggca ctccagcggc acgcccgagt gccgcccgat
     2161 cgaaaggtcc agctcgatca ccgcgtgcgc cgccgcgaat gccgcgtcga catcgccgta
     2221 gccctttcgg atgaccgccg cctcggtgct cagcccggcg tcgaactcgc ccggcgcggc
     2281 gtcagccgag agcagaatcg gcagcggctc catctccatc gtgacgagat cggccgcgtc
     2341 ctcggccgcg tacggatcct ccgcgaacac cgccgccacc ggctcgccga cgtagcgcac
     2401 gcgcccgcgc gcgaggaccg gctggcgata aggctccagg cccttcacgc gcgtgggacg
     2461 gaactcgatc ggagggatcg cggcgacgtc ggccgaggtc cacgccgcga cgcagcccgg
     2521 caactggcgc gccgacgcgg tatcgatcga cacgatccgc gcgtgcgcgt gcgacgatcg
     2581 caccacgcgc atgtgcagct cgcggggaaa cgagacgtcc gcggcgtaac ggcctcgccc
     2641 ggtcagcatc ggcgcatcct cgagccgcgc gaccctgcgg ccgatgacgt tcgtcacgcc
     2701 accacccgcc acgggatcgt ctcgccgccg cgatggacca gcgcccgctc ctcgggtccg
     2761 tcgatcttca tctcggctgg cgccgtccac gcgctgcgct cgagcgtgat cgtctcggtg
     2821 ttcggcggca gcccgtagtg cctcgggccg ttgagcgacg cgaacgcctc gagccggtcg
     2881 agcgcgttct cctcggcgaa gacctgcgcg taggactcga tcgccaccgg cgcgttgaac
     2941 acgcccgcgg cgccgacgac gtcgagcttc ttcgcgaccg ggtgcggcgc cgagtcggtg
     3001 ccgaggaaga agtacggctc tcccgaggtc gccgcggcgc gcagggccgc gcggtccttc
     3061 gcggtcttga tcaccggcgc gcagtacatg tacgggcggt tgccccagcc gagccagtcg
     3121 gtgcgcgtca gcatcaggtg atacggcgtg atcgtgccgc cgacctgtcc ctgcttcgag
     3181 cgcacgaaat cgaccccgat cttcgacgac acgtgctcga ggatgatctt caaccccggg
     3241 aaatcgcgcg cgagcggctc gagctcgcgc tcgatgaaca ccgcctcgcg gtcgaacacg
     3301 tccacgtcgg gatcgatcac ctcgccgtgg atgaggagcc gcatcccgat cttctccatg
     3361 cgctccacca cccggcgaat cctccggtaa tcggagacgc ccgcggccga gttggtggtg
     3421 acgttcgccg ggtagatctt cacgccggtg aacacgcccg ctcggaagcc ggcctcgatc
     3481 tcgtccgggc tcgtctcgtg cgtcatgtag ctcgtcatca gcggctcgaa cttcgcgccc
     3541 cggggtagcg ccttcgcgat gcgctcgcgg tattgctcgg cctccttcac cgtgcagacc
     3601 ggcggcagca ggttcggcat gatgatcgcg cgcccgaaat ggcgcgccgt gaacggcagc
     3661 gccgcgcgca gcatcgcgcc gtcgcgcacg tgcagatgcc agtcgtcggg cttgcggatc
     3721 gtgaggcgat cggtcacgag atgcccatca tccccgcttc gtacgtatcc cgcggcggat
     3781 gcgccttcat cgcgccggtg agcgaggcga cgcgctcgat cttgcgctcg gcgcaccact
     3841 gctccagccc gtcgatcagc gcgatcatcc cggtgggatt gcggaagttc aggtagccga
     3901 cctcgaccgc ggtcgcgccc gcgagcaggt actcgaccac gtcctcggcg cgcatgatcc
     3961 cgccgcagcc gatgatcggg atcttcacgc agcgcgagca ctggtggacc atgcgcagga
     4021 tgatcggctt cacgccgggc cccgagatgc cgccgtagcc ctggccgatg tgcgactgga
     4081 acgtgtcgag gttgatcttc aggccgagga tggtgttcga cacggtgatc gcgtcggcgc
     4141 ccgccttctc cgcggcctcg gcgaccgccg agatatcccc cgcgttcggc gagagcttgg
     4201 cccagagcgg cttgtcggtc gccgcgcggc agcgcttcac caccttgtag gtgtgctcct
     4261 cgttgagcgc gaagttgccg ccgccgggct cgcgcgtcgg gcacgagatg ttgagctcga
     4321 tcgcgtcgac cgcgggcacg ctgacgtcgc gcgccatcgc cgcgaaatcc tcgatctcgg
     4381 tcgaggtgac gctcgcgacg agcggcggcg tgaaccgccg gtactcggga acctggtgct
     4441 cgaggaaata ctcgagcccc ttggtcggca ggccgatcga gttgatcatg ccggcgtcga
     4501 cctcggacac gcgcggcggc gggttgcccg cgcggaagcc gcgcgacacg gtcttggtga
     4561 tcagcgcgcc gaggcggttc aggtcgatga tcttgccgaa ctcggcggag aacgtgcccg
     4621 aggccggcat cacgggattc gccagcttca gctttccgat cttcaccgag agatctacca
     4681 tcccaggcac tcctgcaggt cgaataccgg gccctcgacg cagacgcgcc gcatctcgag
     4741 cttgccggcc acctcgaacg tgcgcacgca gatgtagcag ggcccgaggc cgcacgccat
     4801 gatctgctcc atcgccatct gtcccgggat gccgtgctcc ttcccgagcc gcttcatcag
     4861 ctgcatcagg cggttcgagc cgcaggtgta gaacgcgtcg gccttcttcc cggcgatcag
     4921 gcgccgcagg atcttctcga cgttctcgac cgcgctcgag ccgtcggtgt cgagcacctt
     4981 gacgacggtc cccacgctct cgaacaggtc ggccgccatc gcgagctctt cgctcctcgc
     5041 gctgaggatc gcggtgacgc gcacgccgcg ctcgcccgcg agctggctga tcggcgccat
     5101 ggtcgcgagg cccacgccgc gcccgagcac gaccacgttc ttccaggccg ggtcgagcgt
     5161 gaacccgacg ccgagcggcc cgaccatgtt cagcgtgtcg cccggctgca gcgtcgcgag
     5221 gcctttcgtg ccccggccga ccaccttata aaggaattcg atccgcccgc tcgagcgcgc
     5281 gacgcggtag acgctctgcg gccgccggaa ccacagctgg ccttcgtcgg gcgacgggca
     5341 cagcaggtgg aagaactgcc ccggcgtcgc ggcgagcgcc ttcggcgagg ccttgaccac
     5401 gaggtgcttg tactcggcgt tgatccactc gtgcgcgacc acggggcaca gcgtctcctc
     5461 gaccgggcat tgcggcggcg agtggccggt gtcggcctgg ccgacgacga cggcggcgct
     5521 ggcgtgaacg ggatgggtca tggcgaaagg cgctcgcctg gattggagca cagcgcgcgc
     5581 gcctcctcaa gatgtccgtc gcgttccgcg ccgcgggacg accttcgtgc cgtagttaat
     5641 tgatgctaaa tttccgggca caccaggagg cttcagaaat ggcgaatccc gacctcaggg
     5701 actggctcgc gcagatggac gcggccggcg agctgcagaa ggtgagcggc gccaaccggc
     5761 aggaggagat cggcggcatc gtcgacatct accagcgggc gaccggccgc cccgccctgc
     5821 tgttcgagga catcccgggg tacccgaagg gctaccgcgt cgtcgcgaac gtcctcaccg
     5881 gggtgaagcg catcgcgctc acgttcggca tgccggccga atcgaccgag atggacctcg
     5941 tccactactg gaagaagtac ctcgaggagc agaagacggt cgcgccggcc gtggtgaagt
     6001 cgggcccggt cctcgagaac gtgctctcgg gcaaggaagt gaacctggcg aagatcccga
     6061 cgccgctgtg gcacgaggac gacggcggcc cgtacatcgg caccgcgtgc atggtgatca
     6121 tgaaggaccc ggactcgggc tggatcaact acggcgcgta ccgcgtgcag gcctacgacg
     6181 acccgcggat cgcctcggtg atgtgctcga agggcaagca cggcgatctg ctgatgcgcc
     6241 gctaccacga gcgcggccag cgctgcccgg tcgcggtcgt cgtcggcatg catccgctgc
     6301 tgttcacgat ggcgggcgtc gagctgccct acggcaagaa cgagtacgac gcggtcggcg
     6361 gcatgatggg caagcccgtc gaggtgatcc tcggcccgaa gaccggcctg ccgatcccgg
     6421 cgaacgccga gatcgcgttc gaaggctcga tcgcgcccga cgacctgatc aaggaaggcc
     6481 ccttcggcga gtggacgggc tacttcgccg gcggcgagaa gcaggagccg gtgatccgca
     6541 tcgacacgat gatgcaccgg aacgacccga tcctcctcgg cgccgttccc gccgtgccgc
     6601 cgaacgacga caccttctac cgcggcgcgt tccgctgcgc cgcggtgtgg cggcagctcg
     6661 aggccgcggg cgtcctcggc atccggggcg tgtgggcgca cgaggcgggc ggcagccgca
     6721 tgtggctcac ggtctcgatc aagcagatgt tccccggcca cgcgaagcag gccgggctga
     6781 tcgcctcgca gtgccacgcc ggctcgtacg cgaacaagta cgtcgtggtc gtcgacgacg
     6841 acatcgaccc ggccaacatg aacgacgtgg tctgggcgat gtgcacccgc gtcgacgcgc
     6901 gcgaggacgt cgagatcctg cgcaccagcc gcagcggccc gctcgatccc accagctacc
     6961 cggacggcat cttcgcgttc aactcgcgca tggtgatcga cgcgtgccgc ccgtgggcgc
     7021 ggcgcgagga cttccccaag gtctcgcgct cctccaagga gctcgacgag aggatcaagg
     7081 cgaaatgggc gcacgtgctg ccgcgctgat cgggctgctc ctcgcgggtg cggcctgcgc
     7141 ccaggactac ccgagcaggc cgatccacat ggtggtcgcc ttcccgcccg gcggcatcgc
     7201 cgacttcgcc gcgcgcagcg tctcgaccgg cctcgcggac gcgctgcgcg tcccggtcgt
     7261 ggtcgagaac cgctcgggcg cgggcggctc gatcggcgcc gacttcgtcg cgaaatccgc
     7321 gcccgacggc tacaccgtgc tcgtgacgtc gatctcgcac acgatcaacc cggcgttcat
     7381 gaagaagctc cccttcgaca cgcggcgcga cttcgcgccg gtgatgatga tcgccgacgc
     7441 gcccaacctg ctggtgctgc acccttccgt cccggcgagg acgctcgccg agctgatcga
     7501 ctgggcgcgc gcgcacccgg gcgcgctgac ctacgcgtcc tcgggcgtcg gcacgtcgac
     7561 gcacctgtgc ggcgagctgt tcgcgcagat ggcgaaggtc gacctcgtgc acgtgccgta
     7621 caagggcggc ggtcccgcgg tggccgacct cctcggcggc caggtacgga tgatgttcgc
     7681 gacgctcccg accgtgctcg agcacgcgcg ctccggcagg ctgcgcgcca tcgccaccac
     7741 cggcgcgcag cgcttcgccg gcgcgcgcga gttcccgacg atggccgagg ccggcgccga
     7801 gtcgggactg agcggctacg acgtcagcgc gtggaccggg atgttcgcgc cggcgcacac
     7861 gcccggcgcg gcgatcgacc ggctcgcgca ggagacggcg aagatcctgc gctcccccgc
     7921 cctggaggag cgcctcctcg cgcagggcgc ggaggccgcg gtgaagatgc ccgacgagtt
     7981 cggccgcttc gtcgatgccg agctcgacaa gtggaagaag ctggtcgagt cggcgcactt
     8041 gcgcaccgag gacaagtgaa gccgaacgag ctcacggcgt ccgacgctgc gcggcggatc
     8101 gcgcgccgcg agctgacgtc ggaggcgctg gtgcgcgcgt gcctggagcg catcgaggtg
     8161 cgtgagaaga cggtccgcgc ctgggcgttc atcgatcccg atgctgcgct cgctcaggcg
     8221 cgcgagcgcg accgggagcc cgcgcgcggc gcgctgcacg gcgtgccgat cggcgtgaag
     8281 gacgtgctcg acaccgccga catgccgacc gagatgggct cgccgatcta cgccggctgg
     8341 cggccgcgcg cggacgcggc ctgcgtcgcg atcgcgcgcg cggccggcgc ggtggtcctc
     8401 ggcaagacgg tgaccgccga gctcgccggc atggagcccg gcgcgacgcg caacccgctc
     8461 gacccctcgc gcacgcccgg cggctcgtcg agcggctcgg cggccgcggt ggcggatttc
     8521 atggtgccgc tcgcgctcgg cacgcagacc ggcggctccg tgctgcggcc ggcgtcggtc
     8581 tgcggcgtga tcgggttcaa gccgagcttc ggccgctaca accgctcggg actgaagatg
     8641 gcggccgaga gcctggacac gatcggcacg atggcgcgga agatcgacga catcgagctg
     8701 ctcgacgcgg cgctgacgcg cgcgccggcc cgcccgccgc gtgcgcggga aggcgcgc
//
LOCUS       DATDMW010000365         7870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1225409, whole genome shotgun sequence.
ACCESSION   DATDMW010000365 DATDMW010000000
VERSION     DATDMW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225409"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VI319_15300"
     CDS             <1..242
                     /locus_tag="VI319_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865260.1"
                     /translation="KVPQLFISQRNDPCQGTAPSTVQSVAKRYNIPFVLIQESSGAKG
                     PACGPFSEHSFAGAEKILMDTIAAAVEKGVDQFKQ"
     gene            256..1248
                     /locus_tag="VI319_15305"
     CDS             256..1248
                     /locus_tag="VI319_15305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6865261.1"
                     /translation="MKSRKLGKNGPTVSAIGLGCMGMTGVYGVPNEADAIATIHRALD
                     LGVNFIVSSDAYAAGKNEELIGRALKGRRDRAIIGTKFGNLALSGFKPPEGQSAGHPD
                     YVRSACEDSLRRLGTDVIDLYAMHRMDPKVPIEDTVGAMKKLVEQGKVRHIGLSEAGA
                     QTVRRAHKVHPLASLETEYSLWSRDPERELIPACRELGIAYMAYSPLGRGFLTATIKS
                     PDALVEQDRRRDHPRFNPDNLKRNVALLAPIERIAQAHRVTPAQVALAWVLAQGQDLV
                     PIPGTKHVKYMEQNAAAVDVALSPAENAELAKAFPPGAAAGTRYPEKQMAALGI"
     gene            1263..2117
                     /locus_tag="VI319_15310"
     CDS             1263..2117
                     /locus_tag="VI319_15310"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEY6865262.1"
                     /translation="MSAKTVALLHPGEMGAAVGACLKDAGHRVVWASERRSDATRRRA
                     AAFEDRGTLARALEGADLALSVCPPHGATALARAVAATHYRGVFVDCNAIAPETARKV
                     CAVVQAAGAGFVDGGIIGPPPAQPGKTRLFLSGGPAADVAAMFAGTALEAHALDAPAG
                     AASALKACYAAWTKGATALLAAIRALARHEGVEGALLEEWGRSQPEAPKRSEVVTVQA
                     RKAWRWIGEMEEIGRAFRAAGLPEGFGLAAADVYRALERFKDSGRDPSLDEVIAALAR
                     TQAPARDA"
     gene            2110..3339
                     /locus_tag="VI319_15315"
     CDS             2110..3339
                     /locus_tag="VI319_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6865263.1"
                     /translation="MPDHYDALETRDPEARERALMQALPAHLAHAKKNAPGWARILAG
                     VEPAQVTSRAALARLPVTRKSDLTALQQADPPLGGLAASRRVAKLYVSPGPIHDPECE
                     GRDWWRTARALHAAGFRAGDVAVNTFAYHFTPAGSMMESGAAALGCTVAPTGGGRTEA
                     QVAAIASLRANAYVGTPSFLKLIVEKADELKADIRALVKAEVAAEYLPPALRTAMAGR
                     GIRVRQCYASADLGLIAYESEAMEGMILDEALILEIVRPGTGDPVPEGEVGEVVITSF
                     NADYPLIRFGTGDLSAVMPGASPCGRTNARIRGWLGRADQTTKVKGMFVHPSQVAEVA
                     KRHPEIARARLVVDNPGGADRMTLHCEVRAPREGLERAIAESVREVMRLRGEVALRAP
                     GELPNDGKVIEDAKKYD"
     gene            3332..4519
                     /locus_tag="VI319_15320"
     CDS             3332..4519
                     /locus_tag="VI319_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY6865264.1"
                     /translation="MTEAAGPTRPKPLAGVRVLDLTRLLPGPMATLHLADLGADVVKI
                     EDTGAGDYARAMGHGTPGMSDFFRVLNRNKRAMRLDLKHPRGRDVFLRLAERADVVIE
                     SFRPGVMAKLGLGHDALAARNPRIVVCAITGYGQDGPYRDRAGHDINYMGYAGVGNEI
                     GAEDGPPVVPNFQIADLLGGALVPAMGVLAALLDARATGRGRFVDVAMTDAVLAHAIL
                     PLLGVLEHGATPPRGTGMLSGGLACYNRYRTRDGRWMAVGALERKFWERLCDILRCPE
                     LKASHVVYGEPARAAKEKLAAAFAARTQAEWTAVFAAEDCCVSPVLEMHEALADPQLR
                     ARGMIVGDAEPEGFALPVKMSEFEFDPGSPAPATGEHTAEVLREAGYAASDIEALRRE
                     GVI"
     gene            4520..5356
                     /locus_tag="VI319_15325"
     CDS             4520..5356
                     /locus_tag="VI319_15325"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6865265.1"
                     /translation="MVKNIVAVLALVASGAFAQQPFKEMDTPYVPTPQVVVERMLSMA
                     AVRSGEKLIDLGSGDGRLVITAAKEYGAHGFGVDIDPRLVSRSNQAAEQAGVADRVKF
                     LKQDLFDTDFHDADVLTLYLLPDVNLALRPKILSELRPGTRVVSHDYGMRDWQPDAEA
                     KVPAPDKSVGLRKESMVYLWIVPARVEGDWDVDAGGKRILLSLAQKFQKLSGSVHGAG
                     GEAALGDVRLRGADVRLMLPAGAYGSEPVELQGRASGDTIEGRIVGGGRQIGTFKARR
                     RA"
     gene            5377..5535
                     /locus_tag="VI319_15330"
     CDS             5377..5535
                     /locus_tag="VI319_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865266.1"
                     /translation="MKIARTLGMLLLALWLIVTGLIPLLHLSFHGMNTAMAVLALVSG
                     VLIALGRS"
     gene            5646..6359
                     /locus_tag="VI319_15335"
     CDS             5646..6359
                     /locus_tag="VI319_15335"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEY6865267.1"
                     /translation="MLYGLACYALFLVTFLYAIGFVGDILVPKNLDSGTPGTLAEALV
                     IDVVLLGIFAIQHSVMARPGFKAWWTRFVPRPVERSTYVLLSSLALILLFWQWRPLVA
                     TVWDVESPAARTVLWALYWLGWAIVLVGTFLINHFDLFGLRQVYLNLKREAYTPLGFK
                     TPFLYRIVRHPIMLGFILAFWATPHMSAGHLLFSLATTAYILIALQLEERDLVRFHGV
                     AYQEYQRRTSMIVPLPRRN"
     gene            complement(6413..7084)
                     /locus_tag="VI319_15340"
     CDS             complement(6413..7084)
                     /locus_tag="VI319_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865268.1"
                     /translation="MSIRIIPAVAASIALVAIAAPAQAQKADKNMSFFVTSVGSGKGA
                     DLGGLKGADKHCADLAKAAGSSKKTWHAYLSDSATGGQKAVNARDRIGKGPWMNAKGV
                     VVAKSVDDLHSPNNNLTKQTALDEKGEVVNGRGDKPNRHDMLTGSSPDGRALPGDSDA
                     TCGNWTKSGADGAAMLGHHDRQGLKDDDVSKSWNSSHLTRGGCSQEALKSTGGDGLYY
                     CFASR"
     gene            complement(7168..>7870)
                     /locus_tag="VI319_15345"
     CDS             complement(7168..>7870)
                     /locus_tag="VI319_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6865269.1"
                     /translation="AAIARAHACRTAIPAGEAALIDDWDEQALVLAFADQRAERFAYD
                     EVFHPGDVLRMGGLDWNVLAAPGHDPHAVMFHAPEARVLVSGDALWENGFGVVFPRLF
                     GREHALAETRATLETIAMLDVDTVIPGHGGAFGEVDAAVERALGRVAYYEGDLARLAR
                     HCVKVLLAFALLEKRAMALDELPAYVERVPVYREINRRHLGMAPGALAEWLVSELERA
                     GAARRAGGVLRSASG"
BASE COUNT         1137 a         2741 c         2831 g         1161 t
ORIGIN      
        1 ccaaggtgcc gcagctcttc atcagccagc gcaacgaccc ctgccagggc acggccccct
       61 cgacagtgca gagcgtcgcc aagcgctaca acatcccgtt cgtcctcatc caggagtcga
      121 gcggcgcgaa gggcccggcg tgcggaccgt tctcggagca cagcttcgcc ggcgcggaga
      181 agatcctgat ggacaccatc gccgccgccg tcgaaaaggg cgtcgatcag ttcaagcagt
      241 aaaacgagga gccaaatgaa atcacgcaag ctcggaaaga acggcccgac cgtgtccgcg
      301 atcgggctcg gctgcatggg catgaccggt gtctacggcg tgccgaacga ggccgatgcg
      361 atcgcgacca tccaccgcgc gctcgacctg ggggtcaact tcatcgtcag ctccgacgca
      421 tacgcggcgg gcaagaacga ggagctgatc ggccgcgcgc tgaagggcag gcgcgaccgc
      481 gcgatcatcg gcaccaagtt cggcaatctc gcgctctcgg gcttcaagcc gcccgagggg
      541 cagtcggccg gccaccccga ctacgtgcgc tcggcgtgcg aggattcgct gcggcgtctc
      601 ggcaccgacg tcatcgacct ctacgccatg caccgcatgg atcccaaggt cccgatcgag
      661 gacaccgtcg gggcgatgaa gaagctggtc gagcagggca aggtgcggca catcggcctg
      721 tcggaggcgg gcgcgcagac cgtccggcgc gcgcacaagg tgcacccgct cgcctcgctc
      781 gagaccgagt actcgctttg gagccgcgac cccgagcgcg agctgatccc cgcgtgccgc
      841 gagctcggca tcgcctacat ggcgtattcg ccgctcgggc ggggcttcct caccgccacg
      901 atcaagagtc ccgacgcgct cgtcgagcag gaccggcgcc gcgaccatcc gcgcttcaat
      961 ccggacaacc tgaagcgcaa cgtcgcgctc ctcgccccca tcgagcgcat cgcgcaggcg
     1021 caccgcgtga cccccgcgca ggtggcgctc gcgtgggtgc tcgcgcaggg ccaggacctg
     1081 gtgcccattc ccgggaccaa gcacgtgaag tacatggagc agaacgcggc ggccgtcgac
     1141 gtggcgctgt cgccggccga gaacgcggag ctcgcgaagg cgttcccgcc cggcgcggct
     1201 gccggcacgc gctatccgga gaagcagatg gcggcgctcg gcatctgatc ggcgaggctg
     1261 ccgtgtccgc gaagaccgtc gcgctgctgc atcccggcga gatgggcgcc gcggtcgggg
     1321 cgtgcctgaa agacgcgggt caccgcgtcg tctgggcctc cgagcggcgc agcgacgcga
     1381 cgcgcaggcg cgccgcggcg ttcgaggacc gcggcacgct cgcgcgcgcg ctcgagggcg
     1441 ccgacctcgc gctctcggtg tgcccgcccc atggggccac cgcgctcgcg cgcgcggtcg
     1501 cggccacgca ctaccgcggc gtgttcgtcg actgcaacgc catcgccccc gagaccgcgc
     1561 gcaaggtatg cgcggtggtc caggcggcgg gcgcgggctt cgtcgacggc ggcatcatcg
     1621 ggccgccgcc cgcgcagccc gggaagacgc gcctgttcct ctcgggcggg ccggcggccg
     1681 acgtcgcggc gatgttcgcg ggcaccgcgc tcgaggcgca cgcgctcgac gcgccggccg
     1741 gcgctgcgtc cgcgctcaag gcctgctacg cggcgtggac caagggcgcg accgcgctgc
     1801 tcgccgcgat ccgcgcgctc gcgcgccacg agggcgtgga gggcgcgctg ctcgaggaat
     1861 ggggccgctc gcagcccgag gcgccgaagc gctcggaggt cgtcacggtc caggcgcgca
     1921 aggcctggcg ctggatcggc gagatggagg agatcggccg cgcgttccgc gccgcgggcc
     1981 tgccggaagg gttcgggctc gcggcggccg acgtgtaccg cgcgctggag cgcttcaagg
     2041 actcggggcg cgatccctcg ctcgacgagg tgatcgccgc gctagcgcgg acgcaggcgc
     2101 cggcgcgcga tgcctgatca ttacgacgcg ctcgagacgc gcgatccgga ggcgcgcgag
     2161 cgcgcgctga tgcaggcgct gcccgcgcat ctcgcgcacg cgaagaagaa cgcgccgggc
     2221 tgggcccgca tcctcgccgg ggtcgagccc gcgcaggtga ccagccgcgc tgcgctcgcg
     2281 cggctgccgg tgacgcgcaa gtccgacctg accgcgctgc agcaggccga cccgcctctc
     2341 ggcgggctcg ccgcgtcgcg ccgcgtcgcg aagctctacg tctcgcccgg gccgatccac
     2401 gacccggagt gcgagggacg cgactggtgg cgcacggcgc gcgcgctcca cgcggcgggc
     2461 ttccgcgccg gcgacgtcgc ggtgaacacg ttcgcctatc acttcacgcc cgcgggctcg
     2521 atgatggagt cgggcgcggc cgcgctcggc tgcaccgtgg cgccgaccgg cggcgggcgg
     2581 accgaggcgc aggtcgccgc gatcgcctcc ttgcgcgcaa acgcttacgt cggcacaccg
     2641 tcgttcctca agctgatcgt cgagaaggcc gacgagctga aagccgacat acgcgccctc
     2701 gtgaaggccg aggtcgcggc cgagtacctg ccgccggcac tgcggaccgc gatggccggg
     2761 cgcggcatcc gcgtgcgcca gtgctacgcc tcggcggacc tgggcctgat cgcctacgag
     2821 tccgaggcga tggagggcat gatcctggac gaggcgctga tcctcgagat cgtgcgtccc
     2881 ggcaccggcg acccggtgcc cgagggcgag gtcggcgaag tggtgatcac gagcttcaac
     2941 gctgactatc cgctgatccg cttcggcacc ggcgatctct ccgcggtgat gccgggggcg
     3001 agcccttgcg gccgcaccaa cgcgcgcatc cgcggctggc tggggcgcgc ggaccagacg
     3061 accaaggtga agggcatgtt cgtgcatcct tcccaggtcg cggaggtcgc caagcgccat
     3121 cccgagatcg cacgagcgcg cctggtggtc gacaaccccg gcggcgcgga ccgcatgacg
     3181 ctgcattgcg aggtgcgcgc accgcgcgag ggcctcgagc gcgcgatcgc cgagagcgtg
     3241 cgcgaggtca tgcggctgcg cggcgaggtc gcgctgcgcg cgcccggcga gctgccgaac
     3301 gacggcaagg tgatcgagga cgcgaagaag tatgactgaa gccgcgggtc cgacccggcc
     3361 gaagccgctc gcgggcgtgc gcgttctcga tctgacgcgg ctgctcccgg gcccgatggc
     3421 gacgctgcac ctcgccgacc tcggcgccga cgtcgtcaag atcgaggaca cgggcgcggg
     3481 cgactatgcg cgcgcgatgg gacacgggac tcccgggatg tccgattttt tccgcgtgct
     3541 caaccgcaac aagcgcgcca tgcgcctcga cctcaagcac ccgcgcggtc gcgacgtgtt
     3601 cctgcgcctc gccgagcgcg ccgacgtcgt gatcgagagc ttccggccgg gcgtgatggc
     3661 gaagctcggc ctcggccacg acgcgctcgc cgcgcgcaat ccgcgcatcg tcgtgtgcgc
     3721 catcacgggc tatggccagg acggcccgta ccgcgaccgg gccggtcacg acatcaacta
     3781 catgggctac gccggcgtcg gcaacgagat cggcgccgag gacgggccgc cggtggtgcc
     3841 gaacttccag atcgccgacc tgctcggcgg cgcgctcgtc ccggcgatgg gcgtcctcgc
     3901 ggcgctcctc gacgcgcgcg cgacggggcg cggccgcttc gtcgacgtcg cgatgaccga
     3961 cgcggtgctc gcgcacgcga tcctcccgct gctcggcgtg ctcgagcacg gcgcgacgcc
     4021 gccgcgcgga accggcatgc tctcgggcgg gctcgcctgc tacaaccgct accgcacgcg
     4081 cgacggccgc tggatggcgg tcggcgcgct cgagcggaaa ttctgggagc gcctctgcga
     4141 tatactgcgc tgccccgagc tcaaggcgag ccacgtcgtc tacggcgagc cggcgcgcgc
     4201 ggcgaaggag aagctcgcgg ccgcgttcgc ggcgcgcacg caggccgagt ggacggcggt
     4261 gttcgcggcg gaggactgtt gcgtgagccc ggtgctcgag atgcacgagg cgctcgccga
     4321 cccgcagctg cgcgctcgcg ggatgatcgt cggcgacgcg gagcccgagg gcttcgcgct
     4381 gccggtgaag atgtcggaat tcgagttcga tcccggaagc ccggcgccgg caaccggcga
     4441 gcacactgcc gaggtgctgc gtgaggcggg ctacgccgcg tccgacatcg aggcgctgag
     4501 aagggaagga gtgatctagg tggtgaagaa catcgttgcg gtgctcgcgc tcgtcgcctc
     4561 cggcgcgttc gcgcagcagc cgttcaagga gatggacacg ccgtacgtgc cgacgccgca
     4621 ggtcgtcgtc gagcgcatgc tcagcatggc ggcggtcaga tccggcgaga agctgatcga
     4681 cctcggctcg ggcgacggtc gcctcgtcat cacggcggcc aaagaatacg gcgcgcacgg
     4741 cttcggcgtc gacatcgatc cgcggctcgt gtcccggtcg aaccaggcgg ccgagcaggc
     4801 cggcgtcgcc gaccgcgtga agttcctgaa gcaggacctg ttcgacaccg acttccacga
     4861 cgccgacgtg cttacgctct acctgctccc cgacgtcaac ctggcgttgc ggccgaagat
     4921 cctctccgag ctgcgacccg gcacgcgcgt cgtctcgcac gactacggca tgcgcgactg
     4981 gcagcccgac gccgaggcca aggtgcccgc gcccgacaag agcgtgggct tgcgcaagga
     5041 gagcatggtc tacctgtgga tcgtaccggc gagggtcgag ggcgattggg acgtcgatgc
     5101 cggcggaaag cgcatcctgc tctcgctcgc gcagaagttc cagaagctgt ccgggtcggt
     5161 gcacggcgcc ggcggcgagg ctgcgctggg cgacgtccgc ctgcgcggcg ccgacgtgcg
     5221 gctcatgctt cccgcgggcg cttacgggtc cgaaccggtc gagctccagg ggcgggcgtc
     5281 cggcgatacc atcgaagggc gtatcgtcgg cggcggaagg cagatcggca ccttcaaggc
     5341 gcgccggcgc gcgtaatcga actcgagggg agagatatga agatcgcgag aacgctcgga
     5401 atgctgctgc tcgccctgtg gctcatcgtc acggggctga tcccgctcct gcacttgagc
     5461 ttccacggca tgaacaccgc gatggcggtg ctggcgctcg tgtccggcgt gctgatcgcc
     5521 ctcggcaggt cgtagcccgg cacgcgcgct ggacaagatc ggcggaccag gcgtataacc
     5581 cccgggtcgg cgcagattcg tcagccagtc accacgcggg aggagttatg ggagggatcc
     5641 ttgcgttgtt atacggcctc gcctgctacg ccctgttcct cgtcacgttt ctctacgcga
     5701 tcggcttcgt cggggacatc ttggtgccca agaacctcga ttccgggacg cccggaacgc
     5761 tggccgaagc gctggtcatc gacgtggtcc tgctcggaat cttcgcgatc cagcacagcg
     5821 tcatggcacg gccgggcttc aaggcctggt ggacgcgctt cgtgcctcgg ccggtcgagc
     5881 gcagcactta tgtgttgttg tccagcctcg ctctgattct tctgttctgg cagtggcgtc
     5941 cgttggtggc gaccgtctgg gatgtcgaga gcccagcggc gcgcactgtg ctgtgggcgc
     6001 tgtactggct gggctgggcg atcgtgctcg tgggcacgtt cctcatcaac catttcgatc
     6061 tgttcgggct gcgccaggtt tatctgaacc tgaaacgcga ggcgtacacg ccgctcggct
     6121 tcaagacgcc gttcctgtat cgcatcgtgc gccatcccat catgctcggc ttcatcttgg
     6181 cattctgggc gacgccgcac atgagcgcgg gacacctgtt gttctcgctc gcgacaaccg
     6241 cgtacattct gatcgccttg cagctggaag agcgagatct cgtgcgattc cacggtgtcg
     6301 cgtaccagga gtaccagcgc cggacctcga tgatcgtgcc gctgccgcgc aggaactgag
     6361 cggcacccga aaagaaaagg cccggcgccc gggccttccc gcaaaagggg ccttaccggc
     6421 tcgcgaagca gtagtacagc ccgtcgccgc ccgtgctctt gagcgcttcc tggctgcacc
     6481 cgccgcgcgt caggtgcgac gagttccagg acttcgagac gtcgtcgtcc ttcaggccct
     6541 ggcggtcgtg gtggccgagc atggccgcgc cgtcggctcc gctcttggtc cagttgccgc
     6601 acgtcgcgtc gctgtcgccc gggagcgcgc ggccgtcggg cgaggagccc gtcagcatgt
     6661 cgtggcggtt cggcttgtcg ccgcggccgt tcacgacctc gcccttctcg tcgagcgccg
     6721 tctgcttggt caggttgttg ttcgggctgt gcaggtcgtc gacgctcttc gcgacgacga
     6781 cgcccttggc gttcatccag gggcccttgc cgatgcggtc gcgggcgttg accgccttct
     6841 ggccgccggt cgcgctgtcg ctcaggtagg cgtgccaggt cttcttgctg gagccggccg
     6901 ccttggcgag gtcggcgcag tgcttgtcgg cgcctttcag gccgccgagg tcggcgccct
     6961 tgccggagcc gacgctggtg acgaagaagc tcatgttctt gtcggccttc tgggcctgcg
     7021 ccggcgccgc gatggcgacg agcgcgatgg acgcggcgac ggccgggatg atgcggatgc
     7081 tcatgggtct ctctccttgg ggggtgggct gggaagctgc tccggggtaa acccggcacg
     7141 atcggagatt aatccattcc ttcggcctca gcctgaagcc gagcggagaa cgccgccggc
     7201 ccgccgggcc gcgcccgccc gctccagctc cgatacgagc cattcggcga gcgcgccggg
     7261 cgccatgccg aggtggcggc gattgatctc gcggtagacc ggcacgcgct cgacgtaggc
     7321 tggaagctcg tcgagcgcca tggcgcgctt ctccaggagc gcgaatgcga gcagcacctt
     7381 gacgcagtgc cgcgcgagtc gcgccaggtc gccctcgtaa taggcgacgc gcccgagcgc
     7441 gcgctcgacc gccgcgtcga cctcgccgaa cgcgccgccg tggcccggga tgacggtgtc
     7501 gacgtcgagc atggcgatgg tctcgagcgt ggcgcgggtc tcggcgaggg catgctcccg
     7561 tccgaacagc cgcgggaaca ccacgccgaa gccgttctcc cagagcgcgt cgcccgagac
     7621 gagcacgcgc gcctcgggcg catggaacat cacggcgtgc ggatcgtgcc cgggcgccgc
     7681 gagcacgttc cagtcgaggc cgcccatgcg cagcacgtcg cccgggtgga acacctcgtc
     7741 gtaggcgaag cgctcggcgc gctggtcggc gaaggcgagc acgagcgcct gctcgtccca
     7801 gtcgtcgatg agggcggcct cgcccgcggg gatggccgtg cggcatgcgt gcgcgcgcgc
     7861 gatcgcggcg
//
LOCUS       DATDMW010000366         2967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1849886, whole genome shotgun sequence.
ACCESSION   DATDMW010000366 DATDMW010000000
VERSION     DATDMW010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849886"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VI319_15350"
     CDS             complement(<1..261)
                     /locus_tag="VI319_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865270.1"
                     /translation="MATIVHEGIERRTYPRRANDRTTMALRDNVGSAPVAVSDTDGMR
                     VSWGGIWGGVLVGMGILVLLTALGLAIGATAVDPTDTEAQKIG"
     gene            complement(473..667)
                     /locus_tag="VI319_15355"
     CDS             complement(473..667)
                     /locus_tag="VI319_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865271.1"
                     /translation="MGQPVKTRPILNVADVELMPRPPQFAPKGEAAERYEARVGFIGK
                     PRRLAFIGHQGKGGDYWEGE"
     gene            complement(738..1040)
                     /locus_tag="VI319_15360"
     CDS             complement(738..1040)
                     /locus_tag="VI319_15360"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY6865272.1"
                     /translation="MIVTVFRSRVRPEAQEEYARWAARMSGLAVKMPGYVSHKGFTAP
                     DGERVTIVEFESEEAQRAWAVHAEHVEAKKKGRSDFYLEYRVQVCTVVRDTGFRKK"
     gene            complement(1037..1378)
                     /locus_tag="VI319_15365"
     CDS             complement(1037..1378)
                     /locus_tag="VI319_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEY6865273.1"
                     /translation="MVKISILYPNRPGSRFDLRYYVEKHMPMSIGLLATHPGFRGVSV
                     ERGATGVPPGTAPAYVAMCHFEFDSAEAFLDAFMPHAQTLQGDIANYTDVEPVIQFNE
                     VLISKQAGEKR"
     gene            complement(1404..2600)
                     /locus_tag="VI319_15370"
     CDS             complement(1404..2600)
                     /locus_tag="VI319_15370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6865274.1"
                     /translation="MNRDRRLVFAASFMRAVANGLIGVVAGIYLAELGFDPAAWGVIL
                     GTGVAGTAVGAAGVMFWGDRIGRKRWLIGLGILAAAGGLVFAFSSQMVVLGTAAFLGM
                     LNSLGKDRAAALAIEQAILPSTGAESDRTRAFAWYNVIQDVGHALGAGLAVLPTALRD
                     FAHVDALGSLRAAVIVYALLYAVTAVLYLYLSHAAEAPTAAIRVDVAPQSKSILWKMS
                     FLFALDSFAGGFQGSALFAYFFHAQFGASEATIGLIFVGARIMNAFSHLGAAWLAERI
                     GLVNTMVFTHTPSSILTFTIPFTGSFEIATILFLLREGLSEMDVPTRQSYLMAVVRPE
                     ERTFAGGVTNLARMIARAVAPMFAGTAMQTLALGAPIMAAAAMKVVYDALLYVAFRKV
                     KPPEER"
     gene            complement(2597..>2967)
                     /locus_tag="VI319_15375"
     CDS             complement(2597..>2967)
                     /locus_tag="VI319_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6865275.1"
                     /translation="LEAIGWTPPKVAGSALVHGHCHQKSVLGMGAETALLRRTGLALA
                     LPDTGCCGMAGAFGYDERHYAVSQKIGELAVYPAVRAAPAESRIVANGFACREQIRQG
                     TGRAAMHSAELLAAAVRGEA"
BASE COUNT          463 a         1046 c         1031 g          427 t
ORIGIN      
        1 gccgatcttc tgcgcctcgg tgtcggtcgg gtcgaccgcg gtcgcgccga tcgcgagccc
       61 gagcgccgtc agcagcacca ggatgcccat gccgaccagc acgccgcccc agatgccgcc
      121 ccaggacacg cgcatgccgt cggtgtcgct caccgcgacg ggcgcgctgc cgacgttgtc
      181 gcgcagcgcc atggtggtgc ggtcattggc ccggcgcgga taggtccggc gctcgatgcc
      241 ttcgtggacg atggtagcca ttgcttgtct ccggtgcgtt gtgctcaacc gcttcgcgat
      301 gcaagtaacg gacccggaac gcgaatcccc tggagcaggt cttgcgcgga aacgatcgtg
      361 gcggcggacc cgcgattgtt cgcgaaaacg cgcggagcga tgcgccgaag cgaccgcgcc
      421 gcggcccgga tcctcgtaaa aggtgcagga ggccggcaat aactgccggg cgctactcgc
      481 cttcccagta atcgccgccc ttgccctggt ggccgatgaa tgcgaggcgc cgcggcttgc
      541 cgatgaagcc gacgcgcgcc tcgtagcgct cggcggcctc gcctttcggc gcgaactgcg
      601 ggggccgggg catgagctcg acgtccgcga cgttgaggat ggggcgggtc ttgacgggct
      661 ggcccattgg agattcctcc gatggaaaga atttcaggat agacccgcgt acgcgcgccg
      721 gcgttctcgt gcagcggcta cttcttcctg aaccccgtgt cgcggacgac ggtgcagacc
      781 tgaacccggt actcgaggta gaagtcgctg cggcccttct ttttcgcctc gacatgctcc
      841 gcgtgcacgg cccaggcgcg ctgagcctcc tcgctctcga actcgacgat ggtcacgcgc
      901 tcgccgtcgg gcgcggtgaa ccccttgtgc gagacgtagc cgggcatctt caccgcgagc
      961 ccgctcatgc gcgcggccca gcgcgcgtac tcctcctggg cttcgggtct tacgcgggag
     1021 cggaacaccg tcacgatcat cgcttctctc ctgcctgttt cgagatgagc acttcgttga
     1081 actggatcac cggctcgacg tcggtgtagt tggcgatgtc gccctggagc gtctgcgcgt
     1141 gcggcatgaa cgcgtcgaga aacgcctccg cggagtcgaa ctcgaaatgg cacatcgcga
     1201 cgtacgcagg cgcggtgccc ggcggcacgc cggtcgcgcc gcgctcgacc gagacgccgc
     1261 ggaatcccgg gtgcgtcgcc agcagcccga tcgacatcgg catgtgcttc tcgacgtagt
     1321 aacgcaggtc gaagcgcgag ccgggccggt tgggatagag gatgctgatc ttcaccacga
     1381 aggcgattct accgccggcg ccgtcaacgc tcctccggcg gcttcacctt ccggaacgcg
     1441 acgtagagca gcgcgtcgta gacgaccttc atcgccgcgg ccgccatgat gggcgcgccg
     1501 agcgcgagcg tctgcatcgc ggtgccggcg aacatgggcg cgacggcgcg cgcgatcatg
     1561 cgcgcgaggt tggtgacgcc gccggcgaag gtgcgctcct cggggcgcac gacggccatg
     1621 aggtagctct ggcgcgtcgg cacgtccatc tccgagaggc cctcgcgcag caggaacagg
     1681 atcgtcgcga tctcgaagct gccggtgaac gggatcgtga acgtgaggat cgacgacggg
     1741 gtgtgcgtga acaccatggt gttgacgagg ccgatgcgct cggcgagcca ggcggcgccg
     1801 aggtgcgaga acgcgttcat gatgcgcgcg ccgacgaaga tcagcccgat ggtcgcctcc
     1861 gacgcgccga actgcgcgtg gaagaagtac gcgaacagcg cgctcccctg gaagccgccg
     1921 gcgaagctgt cgagcgcgaa caggaacgac atcttccaca ggatcgactt gctctgcggc
     1981 gcgacgtcga cgcggatcgc ggcggtcggc gcctcggcgg cgtgcgacag gtacaggtac
     2041 agcaccgcgg tcacggcgta caggagcgcg tagacgatca ccgccgcgcg cagcgacccg
     2101 agcgcgtcga cgtgcgcgaa atcgcgcagc gcggtcggca gcaccgcgag ccccgcgccg
     2161 agcgcgtgac cgacgtcctg gatcacgttg taccaggcga aggcgcgcgt gcggtcgctc
     2221 tccgcccccg tcgacggcag gatcgcctgc tcgatcgcga gcgccgccgc gcggtccttg
     2281 ccgagcgagt tcagcatgcc caggaaggcc gccgtgccga gcacgaccat ctgcgacgag
     2341 aacgcgaaca cgaggccgcc cgccgcggcg aggatcccca gcccgatcag ccagcgcttg
     2401 cgcccgatgc ggtcgcccca gaacatcacg cccgccgcgc cgacggcggt gccggcgacg
     2461 ccggtgccga ggatcacgcc ccaggccgcc ggatcgaagc cgagctcggc gagatagatc
     2521 ccggccacga cgccgatcag cccgttcgcg accgcgcgca tgaagctcgc ggcgaagacc
     2581 aggcgccggt cgcggttcac gcctcgcccc gcaccgcggc cgcgagcagc tcggccgagt
     2641 gcatcgccgc gcgccccgtc ccctggcgga tctgctcgcg gcacgcgaac ccgttcgcga
     2701 cgatgcgcga ctccgcgggc gccgcgcgca ccgccgggta gaccgcgagc tcgccgatct
     2761 tttgcgacac cgcgtagtgg cgctcgtcgt agccgaacgc gcccgccatg ccgcagcacc
     2821 cggtgtcggg cagcgcgagc gcgagccccg tcctgcgcag gagcgcggtc tcggcgccca
     2881 tcccgaggac cgacttctgg tggcagtgcc cgtgaaccag cgccgagccc gcgaccttcg
     2941 gcggcgtcca gccgatcgcc tcgagga
//
LOCUS       DATDMW010000367         7828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_975524, whole genome shotgun sequence.
ACCESSION   DATDMW010000367 DATDMW010000000
VERSION     DATDMW010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975524"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /gene="zapD"
                     /locus_tag="VI319_15380"
     CDS             complement(<1..554)
                     /gene="zapD"
                     /locus_tag="VI319_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEY6865276.1"
                     /translation="MITYEYPLNERIRTLLRLEDLFERGKYFAERPEPLEHHAALLTL
                     FEILEVAGRADLKSDLIQELERQKQMLVSFRNNPQIAEDVLNQLIRDIERTSQALLGM
                     TGKIGQYLRENEWLMSIKQRTAIPGGACEFDLPSYHYWLHRPAGERIGQLAAWTSPLY
                     PLRDGSAIILRILRESGKPQSLSAP"
     gene            complement(630..1250)
                     /gene="coaE"
                     /locus_tag="VI319_15385"
     CDS             complement(630..1250)
                     /gene="coaE"
                     /locus_tag="VI319_15385"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059619.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY6865277.1"
                     /translation="MSRYVVGLTGGIGSGKSEVARLFAERGAEVVDTDVIAHELTAAG
                     GAAIGPIRAAFGADVIGSDGALDRAAMRRLAFSDPAARGTLESILHPLIRRESDARIA
                     RGTGPYVVLVVPLLVEAGVDRRRYQRVLVVDAPEAEQVARVAARNRLEPTEVHAIMAA
                     QAARAERLSHADDVIDNSGPIRALEPQVDRLHALYLAQARQIGAAR"
     gene            complement(1247..2155)
                     /locus_tag="VI319_15390"
     CDS             complement(1247..2155)
                     /locus_tag="VI319_15390"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY6865278.1"
                     /translation="MSASISLFSHPAVFPWVAAVLGLAVGSFLNVVIHRLPKMMERDW
                     EAQCAELAQNKAAGAGAPAHAPPVPGAEVLAPYNLFVPRSACPACGHRIGALENVPLV
                     SYAVLRGRCSKCGTRISARYPLVELLAAAGAGYCAVRLGFGLAAFAAMIYVWCLIAAS
                     FIDFDTQLLPDAITLPLVWLGLLVNIPGTFTDLPSAVIGATAGYLSLWSVYWIFKLLT
                     GKEGMGFGDFKLLAAIGAWCGWQMLPLVILMSSVIGAAVGMVLIVLSRLGRNVPIPFG
                     PYLAAAGVVALFWGPQVTRHYLNQFL"
     gene            complement(2226..3449)
                     /locus_tag="VI319_15395"
     CDS             complement(2226..3449)
                     /locus_tag="VI319_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY6865279.1"
                     /translation="MATAAAAKLKVKELQFAWEGKDRHGKVVKGEMRAAGDHVVNAQL
                     RRQGIAVTKIRRLRARSGGKVKEKDITLFTRQMATMMKAGVPLLQAFDIVGKGHSNPA
                     VARLLLELKTEVETGSSLAAAFRKYPLYFDALFCNLIAAGEAAGILETLLDRLATYKE
                     KILAIKAKIKSAMFYPTAIIVVAFVITAVIMIFVIPAFKQVFTSFGADLPAPTLFVMG
                     VSDAFVKYWYLIFGAVIGGVWGLLALWKRSIPVQIFMDRVMLRLPVFGDLVKKSTIAR
                     WTRTLSTMFAAGVPLVEALDSVGGASGNYVYVMATKEIQQKVSTGSSLTVAMQEANVF
                     PAMVIQMVSIGEESGALDNMLGKVADFFEAEVDEAVKALSSLMEPMIMVVLGVLIGGM
                     VIAMYLPIFKIGQAV"
     gene            complement(3470..5179)
                     /gene="pilB"
                     /locus_tag="VI319_15400"
     CDS             complement(3470..5179)
                     /gene="pilB"
                     /locus_tag="VI319_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEY6865280.1"
                     /translation="MAANVRTLGRSGLARALVQHGKLRDSEVDALISDASSAGASFVE
                     QLVGSRRMSAIEVAEFAAATFGMPLLDLAAADTARFPAGVVEGRLVQSRRVLPLHRRG
                     NRLFVAISDPSNTQALDDVKFQSSLAIEPVVVEDDKLSAAIKKFVESTDTSLKGMIDE
                     DLELEFSHEDEQAPQEEHSEADDAPIVKFIQKVLIDAISDGASDIHFEPYEKHYRVRF
                     RVDGVLRDVSQPPLALKDRIASRIKVISRLDISEKRVPQDGRMKLVLAKGRAIDFRVS
                     TLPVLHGEKIVMRILDPTMAAIGIDALGYEPEQKQALLDAIRRPYGMILVTGPTGSGK
                     TVSLYTCLNLLNEPGVNISTAEDPAEIMLPGINQVNVHEKAGLTFAVALKAFLRQDPD
                     VIMVGEVRDLETAEIAVKAAQTGHLVLSTVHTNDAPTTLTRLLNMGVAPFNIASSVIL
                     ITAQRLVRRLCTCKAPVTIPREALESAGFAGEELEGSWQAYRAVGCERCKGTGYRGRV
                     GVYQVMPISEEINRIIMRGGNAIDIADQARREGVRDLRQSGLLKVKQGLTSIEEVMAS
                     TNE"
     gene            complement(5303..6586)
                     /locus_tag="VI319_15405"
     CDS             complement(5303..6586)
                     /locus_tag="VI319_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEY6865281.1"
                     /translation="MDDIPIAAQLAALAVLLVLSAFFSIAETSMMALNRYRLKAMVRA
                     GHRGAKVVAELLAQIDRLLGVILLGNTLVAAGAATLAALIAHTLFGDYRWVPAASALA
                     VTFALLVFSEITPKVIGAAYAERIALAVGYVLKPMLWVLYPVVWLVNLFVRPLLFVFR
                     VKTRAAQPQRLSMEDLRLLVLESGQYMPRKHQSIVLNLFGLEAITVEDVMTARALIEA
                     VDLDAPLETIVAQLSTAYHTRLPVYRGELGNIAGVLHLRKVLGLAQSGSPDREGLEQL
                     LEQPYFVPASTPVFAQLQYFQENRQRLALVVDEYGEIQGLVTMEDIIEEFVGEFTTTA
                     PMKSASYKWDKDGSALVEGASSLRELNRKLGLALPLSGPKTLNGLILEHLRDIPEAGV
                     SVKIAGVPMEIVHTQDRVVRTVRLFRPRATEALGA"
     gene            complement(6648..7469)
                     /gene="ccsA"
                     /locus_tag="VI319_15410"
     CDS             complement(6648..7469)
                     /gene="ccsA"
                     /locus_tag="VI319_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY6865282.1"
                     /translation="MPSILLYVATTTLYAALAFHFWRTRWREPGPASIEPWERWAIVA
                     PLALHAWLVYEAVLAAPELRFGFGQALSMMLWLTVLLYWIESLFLRLEGMQPLVLGVA
                     AVCVPLPLIFPGLSIHAPTQSLEFRLHLMLGMSAYSLFTIAALHALLMTLLERRLHGG
                     ALSGPFSALPPLLTMESLLFRIIFFGFVLLTLTLASGFVFSEELFGRPVRFEHKSVFA
                     ITSWVIFAALLAGRRLYGWRGRVALRWTLAGFLMLLLAYVGSRFVLEVILRRGGV"
     gene            7505..>7828
                     /locus_tag="VI319_15415"
     CDS             7505..>7828
                     /locus_tag="VI319_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEY6865283.1"
                     /translation="MLENLTQRLSRVVKTIRGEARLTESNIAEALREVRVALLEADVA
                     LPVVRDFIARVREKALGQDVVGSLTPGQALVGVVHRELAALMGGENAALDLAVTPPAV
                     VLMAGL"
BASE COUNT         1330 a         2642 c         2653 g         1203 t
ORIGIN      
        1 ggcgccgaca ggctttgcgg cttgcccgac tcgcgcagga tgcgcaggat gatcgccgag
       61 ccgtcccgca aggggtagag cgggctcgtc caggcggcga gctggccgat cctttccccg
      121 gcgggccgat gcagccagta gtggtaggag ggcagatcga actcgcacgc gccgcccggg
      181 atggccgtgc gctgcttgat cgacatgagc cactcgttct cgcgcaggta ctggccgatc
      241 ttccccgtca tgccgagcag cgcctggctc gtgcgctcga tgtcgcggat gagctgattc
      301 agcacgtctt ccgcgatctg cgggttgttg cggaacgaga cgagcatctg cttctgacgc
      361 tcgagctcct ggatgaggtc cgatttcaga tcggcgcggc ctgccacttc gaggatctcg
      421 aacagggtca gcagggcggc gtggtgctcg agcggctcgg ggcgctccgc gaaatacttg
      481 ccgcgctcga acagatcctc cagtcgcagc agcgtgcgga tgcgctcgtt gagaggatac
      541 tcgtatgtga tcactgtctc ggccgaggct gaacctgtcc ggagcctcgg gaaattctcc
      601 ccgattcccc cgggattgac aaccggagac taccgcgccg cgccgatttg ccgcgcctgg
      661 gcgagataga gagcgtgcag gcgatcgacc tgcggctcga gggcgcggat ggggccgctg
      721 ttgtcgatga cgtcgtccgc gtgcgacagt cgctcggcgc gcgccgcctg cgccgccatg
      781 atggcgtgca cctcggtcgg ctccagccga ttgcgcgccg ccacgcgggc gacctgctcc
      841 gcttccgggg cgtcgacgac cagcacgcgc tgatagcgcc gccggtcgac gcccgcctcc
      901 accaggagcg gcaccaccag cacgacgtac ggacccgtcc cgcgggcgat ccgcgcgtcg
      961 ctctcgcgtc ggatcagggg atgcaggatg gattcgagcg tgccgcgcgc cgcggggtcc
     1021 gagaacgcga ggcgtcgcat cgcggcgcga tcgagcgccc cgtccgatcc gatcacgtcc
     1081 gcgccgaagg cggcgcggat gggcccgatg gcggccccgc ctgcggcggt gagctcgtgc
     1141 gcgatgacat ccgtgtcgac gacctcggcg cctcgctcgg cgaacaaccg ggccacctcg
     1201 ctctttccgc tgccgatccc gcccgtcagg ccgacgacgt agcggctcac aggaactggt
     1261 tgaggtagtg ccgcgtgacc tgcggccccc agaacagcgc caccacaccg gcagcggcga
     1321 gatagggccc gaacgggatg gggacgttcc gaccgaggcg cgacagcacg atgagcacca
     1381 tgccgaccgc ggcgccgatc accgacgaca tgaggatcac cagcggcagc atctgccagc
     1441 cgcaccaggc gccgatcgcg gcgaggagct tgaagtcgcc gaaccccatg ccttccttgc
     1501 ccgtgaggag cttgaagatc cagtacacgc tccagagcga gagatagccg gccgtggcgc
     1561 cgatcaccgc cgacggcagg tcggtgaacg ttccggggat gttgacgagc aacccgagcc
     1621 agacgagcgg gagcgtgatc gcatcgggca gcagctgggt atcgaagtcg atgaagctcg
     1681 ccgcgatcag gcaccagaca tagatcatcg ccgcgaaggc cgcgaggccg aagcccaggc
     1741 ggacggcgca atatcctgcg cccgccgccg caagcagctc gaccagcggg tagcgcgcgc
     1801 tgatgcgcgt gccgcacttc gagcagcggc cgcgaaggac cgcgtacgag acgagcggga
     1861 cgttttccag ggcgccgatg cgatgtccgc acgccgggca ggccgagcgc ggcacgaaga
     1921 ggttgtacgg cgccagaacc tcggctccgg gcaccggcgg agcgtgggcg ggcgcgccgg
     1981 cgccggcggc cttgttctgt gcgagctcgg cgcattgggc ttcccagtcg cgctccatca
     2041 tcttcggcag acggtggatg accacgttga ggaagcttcc cacggcgagg ccgagcaccg
     2101 cggccaccca cgggaacacc gcagggtgcg agaagaggct gatcgaggcg ctcatcggcg
     2161 ggaagaaaaa agggttacgg tacacccgca accctctttc ggggaaacgg cggcgcgtcg
     2221 cggcgtcaga cggcctggcc gatcttgaat atcggcagat acatcgcgat caccatgccg
     2281 ccgatcagca cgccgagcac caccatgatc atcggctcca tgaggctcga cagggccttc
     2341 accgcctcgt ccacctccgc ctcgaagaag tcggcgacct tcccgagcat gttgtccagc
     2401 gcgcccgact cctcgccgat ggagaccatc tggatgacca tggcagggaa aacgttcgcc
     2461 tcctgcatgg caacggtgag gctcgagccg gtgctcacct tctgctggat ctccttcgta
     2521 gccatgacgt agacgtagtt gcccgacgcc cctccgaccg agtcgagcgc ctcgacgagc
     2581 ggaaccccgg ccgcgaacat cgtggacagc gtgcgtgtcc agcgggcgat ggtcgacttc
     2641 ttcaccaggt cgccgaagac gggcaggcgg agcattaccc ggtccatgaa gatctgcacc
     2701 gggatcgagc gcttccagag cgcgagcagc ccccacacgc cgccgatgac ggcgccgaag
     2761 atgaggtacc agtacttcac gaaggcgtcc gagaccccca tcacgaacag cgtgggcgcc
     2821 ggaagatcgg cgccgaagct cgtgaacacc tgcttgaacg ccgggatcac gaagatcatg
     2881 atcaccgccg tgatgacgaa cgccacgacg atgatggcgg tcgggtagaa catggcggac
     2941 ttgatcttcg ccttgatcgc gaggatcttc tccttgtagg tcgccagccg gtcgagcagc
     3001 gtctccagga taccggccgc ctcgccggcc gcgatcaggt tgcagaacag ggcgtcgaag
     3061 tacagggggt acttgcggaa cgcggcggcg agcgaggagc ccgtctcgac ctccgtcttc
     3121 agctcgagga gcaggcgcgc cacggccggg ttggagtgac ccttgccgac gatgtcgaag
     3181 gcctgcagga gcggcacgcc tgccttcatc atggtggcca tctggcgggt gaacagcgtg
     3241 atgtccttct ccttgacctt cccgccggag cgcgcgcgca gccgcctgat cttggtgacg
     3301 gcgatgccct ggcgtcgcag ctgggcgttc accacgtgat cgcctgcggc gcgcatctcg
     3361 cctttcacga ccttgccgtg acggtccttg ccttcccagg cgaactggag ctccttgacc
     3421 ttcagcttcg ccgcagcagc agttgccatg acgcttctcc ctgactactt cattcgttgg
     3481 tcgacgccat cacttcctcg atggacgtca gcccttgttt gaccttgaga agacccgact
     3541 ggcgcagatc ccgcacgccc tcgcgccggg cctggtccgc gatgtcgatg gcattgccgc
     3601 cgcgcatgat gatccggttg atctcctcgg agatcggcat cacctggtac acgccgacac
     3661 ggccgcggta gcccgttccc ttgcagcgct cgcacccgac cgcgcgatac gcctgccagc
     3721 tgccttcgag ctcttctccc gcgaatccgg cgctctcgag cgcttcccgc gggatggtaa
     3781 ccggcgcctt gcacgtgcac aagcggcgca ccagacgctg cgcggtaata agtatcacgc
     3841 tcgaggcaat gttgaacggc gcgacaccca tgttcagcag gcgcgtgagc gtcgtcggcg
     3901 cgtcgttcgt gtgcaccgtc gacagcacga gatggccggt ctgcgcggcc ttgacggcga
     3961 tctccgccgt ctccaggtcg cgcacctcgc ccaccatgat cacatcgggg tcctggcgca
     4021 ggaaggcctt gagggcgacc gcgaacgtca gtcccgcctt ctcgtgcacg ttcacctggt
     4081 tgatcccggg cagcatgatc tcggcgggat cctcggcggt cgagatgttg acccccggct
     4141 cgttgagcag gttgaggcag gtgtacagcg acaccgtctt gccgctcccg gtcgggccgg
     4201 tcacgaggat catgccgtag gggcggcgta tcgcgtcgag cagcgcctgc ttctgctccg
     4261 gctcgtaacc gagcgcgtcg atgccgatcg ccgccatggt gggatcgagg atgcgcatga
     4321 cgatcttctc gccgtgcagc acgggcagcg tgctcacgcg gaagtcgatc gcgcgccctt
     4381 tcgcgagcac gagcttcatg cggccgtcct gcggcacgcg cttctcggag atgtccagcc
     4441 gcgagatcac cttgatgcgc gaggcgatac ggtccttgag cgcgaggggc ggctgcgaca
     4501 cgtcgcgcag tacgccatcg acgcggaagc gcacgcggta gtgcttctcg tagggctcga
     4561 aatggatgtc ggaggcgccg tcgctgatcg cgtcgatcag caccttctgg atgaacttga
     4621 cgatgggcgc gtcgtcggcc tcgctgtgtt cctcctgcgg cgcctgctcg tcctcgtgcg
     4681 agaactcgag ctccaggtcc tcgtcgatca tccccttgag ggacgtatcg gtcgactcga
     4741 cgaacttctt gattgcagcc gagagcttgt cgtcctcgac cacgaccggc tcgatcgcga
     4801 ggctcgactg gaacttcacg tcgtcgaggg cctgcgtgtt ggacggatcc gagatcgcga
     4861 cgaacaggcg attaccgcgc cggtgcaagg gcaggacgcg ccggctctgc accagcctgc
     4921 cttcgaccac gccggcgggg aagcgcgccg tgtcggctgc ggcgagatcg agcagcggca
     4981 tgccgaaggt cgccgcggcg aattcggcga cctcgatggc gctcatccgg cggctgccga
     5041 cgagctgctc gacgaacgag gcgcctgccg agctcgcgtc ggagatcagc gcatcgacct
     5101 cgctgtcgcg caacttgccg tgctgcacga gggccctcgc gaggccgctc ctgccgagcg
     5161 tgcggacgtt ggccgccatg cgggaccatc agccggaaaa aggatgctgg atgatgcaac
     5221 gcgcggagct ttttgtaaag cctgaatttc ctgcgctaaa tccatgagaa atatgcgcaa
     5281 tcaggctccg gaaacacctc cttcaggcgc ccagggcctc cgtcgcgcgc gggcggaaca
     5341 ggcgcaccgt gcgcaccacg cggtcctgcg tgtgcacgat ctccatcggc acgccggcga
     5401 tcttcacgct gaccccggcc tcggggatgt cgcgcaggtg ctcgaggatc aggccgttca
     5461 gcgtcttcgg acccgagagc gggagcgcga gcccgagctt gcggttgagc tcgcgcaggc
     5521 tgctcgcacc ctcgaccagc gcgctgccgt ccttgtccca cttgtagctc gccgatttca
     5581 tcggcgcagt cgtcgtgaat tcgccgacga actcctcgat gatgtcctcc atcgtcacca
     5641 ggccctggat ctcgccgtac tcgtcgacca ccagggccag gcgctgccgg ttctcctgga
     5701 agtactgtag ctgcgcgaac accggggtcg acgccggaac gaaatacggc tgctcgagca
     5761 gctgctcgag gccttcgcgg tcgggcgagc ccgactgcgc caggcccagc accttgcgca
     5821 ggtgcaggac cccggcgatg ttgccgagct cgccgcgata caccgggagc cgcgtgtgat
     5881 acgcggtcga gagctgcgcg acgatggttt caagcggcgc gtcgaggtcg accgcctcga
     5941 tgagcgcccg cgccgtcatc acgtcctcga ccgtgatcgc ctccaggccg aagaggttga
     6001 gcacgatgct ctggtgcttg cgcggcatgt attgcccgga ctcgagcacg agcagtcgca
     6061 gatcctccat cgagagccgc tgcggctgcg cggccctcgt cttcacgcga aacacgaaca
     6121 gcagcggccg cacgaacagg ttcacgagcc agaccacggg gtagagcacc cagagcatcg
     6181 gcttcagcac gtagccgacc gcgagcgcga tgcgctcggc gtacgcggcg ccgatcacct
     6241 tgggcgtgat ctcggagaag acgagcagcg cgaacgtcac cgcgagcgcg ctcgccgcgg
     6301 gcacccagcg gtagtcgccg aacagcgtgt gcgcgatcag ggcggcgagc gtggcggcgc
     6361 cggcggcgac cagcgtgttt ccgagcagga tcaccccgag cagccggtcg atctgcgcga
     6421 gcagctcggc cacgaccttc gcgccgcggt gtcccgcgcg caccatcgcc ttcaggcgat
     6481 agcggttcag cgccatcatg ctcgtttcgg cgatggagaa gaacgcggag aggaccagca
     6541 gcacagcgag cgcggcgagc tgcgctgcta tggggatgtc gtccaaggcg tgcgcctaga
     6601 tgacggagac gatttcagta taccgcagcg gtccgcggcg cggcgcctca gactccgccg
     6661 cgccgcaata tcacctcgag cacgaagcgg ctgccgacgt aggccagcag cagcatcagg
     6721 aagccggcga gcgtccagcg cagcgcgacg cgcccgcgcc agccgtagag ccggcgcccc
     6781 gcgagcagcg cggcgaagat cacccacgag gtgatcgcga acaccgactt gtgctcgaag
     6841 cgcaccggtc gaccgaacag ctcctcggag aagacgaacc cgctcgcgag cgtcagcgtg
     6901 agcaggacga agccgaagaa gatgatgcgg aagagcaggc tctccatcgt caggagcggc
     6961 ggcagcgccg agaacgggcc ggacagcgcg ccgccgtgca ggcgccgctc gagcagcgtc
     7021 atgagcagcg cgtgcagcgc ggcgatggtg aacaggctgt atgccgacat gccgagcatg
     7081 aggtgcagcc ggaactcgag cgactgggtc ggcgcgtgga ttgagagtcc gggaaagatc
     7141 agcggcaggg gcacgcacac cgccgcgacg ccgagcacca gcggctgcat gccttccagg
     7201 cgcaggaaca ggctctcgat ccagtagagc agcaccgtca gccacagcat catcgagagc
     7261 gcctggccga agccgaagcg cagctcgggc gcggcgagca ccgcctcgta gacgagccag
     7321 gcgtggagcg cgagcggcgc gacgatcgcc caccgctccc acggctcgat cgacgccggc
     7381 ccgggctcgc gccagcgcgt ccgccagaaa tggaacgcga gcgccgcgta cagggtcgtc
     7441 gtcgcgacgt agagtagaat ggacggcatc ccgccagttt acccgttcca cgcagggccg
     7501 ctccatgctc gagaacctca cccagcgact gtcgcgcgtc gtgaagacga tccgcggcga
     7561 ggcgcgcctg accgagtcga acatcgccga ggcgctgcgc gaggtgcgcg tcgcgctgct
     7621 cgaggccgac gtcgcgctgc cggtggtgcg cgacttcatc gcccgggtgc gcgagaaggc
     7681 gctcggccag gacgtggtcg gcagcctgac cccgggccag gcgctcgtcg gcgtcgtgca
     7741 ccgcgagctc gcggcgctga tgggcgggga gaacgcggcg ctggatctcg cggtgacgcc
     7801 gccggccgtc gtgctgatgg cggggctc
//
LOCUS       DATDMW010000368         2954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1102750, whole genome shotgun sequence.
ACCESSION   DATDMW010000368 DATDMW010000000
VERSION     DATDMW010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102750"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VI319_15420"
     CDS             <1..615
                     /locus_tag="VI319_15420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HEY6865284.1"
                     /translation="REMAIAAPFQDVLAAGGGLPTSAPGRIQHVQDFKPGMLLATHNP
                     LDVAIEGEGFFEIQTDAGPRYTRQGNFRLDGVGRLVNDAGSPVMSDGGEVVLKSGQVS
                     IDGQGRIQEGDRPAGRLRIVRFNDPHTLTHVGSGLYAMGDGSSVRSDGYANVRQGFLE
                     GSNVNSTQEMVKMIETVRHFESGQKVIQAYDDMLGQALSKLGEF"
     gene            627..1403
                     /gene="flgG"
                     /locus_tag="VI319_15425"
     CDS             627..1403
                     /gene="flgG"
                     /locus_tag="VI319_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY6865285.1"
                     /translation="MIDSLYIGATGMAAQQTNVDTISNNLANVNTPTYKKSRVVFEDL
                     MQQASRTGAVDASGQPLATTGLGTALSTSGKIFTDGEIVKSANPLDVAIRGSGFLEVL
                     MPDGSSGYTRSGTLQVNKDGMLTTSDGNLLKAAIQIPPDAKNVTIDANGNVSVTLPNQ
                     TDPDPLGQLELVNFINPAGLNPVGSNLYAATDKSGDPVQGRPGDDGFGTIAQGFTEAS
                     NVNLTDELVNLIVAQRAYQVNAKVIQASDDMLNITNNLRR"
     gene            1412..2104
                     /gene="flgA"
                     /locus_tag="VI319_15430"
     CDS             1412..2104
                     /gene="flgA"
                     /locus_tag="VI319_15430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY6865286.1"
                     /translation="MLRPVPAIAVLLALVATGARGADPVVEAARAHLERALAPAHEQV
                     IVEPLPGRQAAPLPAGGVSLETRGCGRETPARRMCVWVDVMVDGARRSSVAVWFRVEA
                     KRKVLVATDPVPADAEFDPRAFALELRDVAALPGAPLATAAHAGRLRVKRPLEPGQVV
                     LERDVTPIPAVARNQEVTIRLQTGGILIEAPGVALSEGRVGATVRVRNPRSGEIYQAR
                     VVDDGVLAVTLR"
     gene            2116..2700
                     /locus_tag="VI319_15435"
     CDS             2116..2700
                     /locus_tag="VI319_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY6865287.1"
                     /translation="MPTRACLAVVLAVLAADAALPAAAESLYQPGQYQPLVADRRAYR
                     PGDVLTVQVYENSSAQSTADTTTKKDGSLNLSLKTPAINKAAGAQLSDDFNGTGKTQR
                     TGKLLAQFTVVVQGVDANGLLIIKGQQLIDVNDEKQEIRLEGKVRPIDIADNNTILST
                     RIADARISYMGDGVLGEHQRPGILTRILSWLGIL"
     gene            2697..>2954
                     /locus_tag="VI319_15440"
     CDS             2697..>2954
                     /locus_tag="VI319_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY6865288.1"
                     /translation="MKAALVPLLAAVLAAPASAQPGDGVRIKDLGRIDGVRENQLVGY
                     GIVTGLAGSGDSSRSRATRQSIANLLSQFGLYVPNDQVQSRN"
BASE COUNT          510 a         1035 c         1013 g          396 t
ORIGIN      
        1 cgcgagatgg cgatcgcagc tccgttccag gacgtgctcg ccgccggcgg cggcctgccg
       61 acatcggcgc ccggccggat ccagcacgtg caggacttca agcccggcat gctgctcgcc
      121 acgcacaacc cgctcgacgt cgcgatcgaa ggcgagggtt tcttcgagat ccagaccgac
      181 gcgggcccgc gctacacgcg ccagggaaat ttccggctcg acggggtcgg gcgcctcgtc
      241 aacgacgccg gctcgccggt gatgagcgac ggcggcgagg tcgtcctgaa gtccggtcag
      301 gtctccatcg acggccaggg ccgcatccag gaaggcgacc gcccggcggg ccggctgcgc
      361 atcgtgcgct tcaacgaccc gcacaccctg acgcacgtcg gctcgggcct gtacgcgatg
      421 ggcgacggat cgagcgtgcg cagcgacggc tacgcgaacg tgcgccaggg cttcctcgag
      481 ggctccaacg tcaactcgac gcaggagatg gtgaagatga tcgagaccgt gcgccacttc
      541 gagtcggggc agaaggtgat ccaggcctac gacgacatgc tggggcaggc gctcagcaag
      601 ctcggcgaat tctaaggagg aagacgatga tcgattccct gtatatcgga gcgaccggga
      661 tggcggcgca gcagaccaac gtcgacacca tctccaacaa cctggccaac gtcaacacgc
      721 cgacgtacaa gaagtcgcgc gtggtgttcg aggacctgat gcagcaggcg tcgcggaccg
      781 gggcggtcga cgcgagcgga cagccgctcg cgaccaccgg gctcgggacc gcgctctcga
      841 cctccggcaa gatcttcacc gacggcgaga tcgtgaaatc cgccaatccg ctcgacgtcg
      901 ccatccgtgg cagcggtttc ctcgaggtcc tgatgcccga cggatcctcg ggctataccc
      961 gcagcggcac gctgcaggtc aacaaggacg gcatgctcac cacgagcgac ggcaacctgc
     1021 tcaaggcggc catccagatt ccgcccgacg cgaagaacgt cacgatcgac gcgaacggca
     1081 acgtgagcgt gacgctgccg aaccagaccg atcccgaccc gctcgggcag ctcgagctgg
     1141 tgaacttcat caacccggcc gggctgaacc cggtcggcag caacctctat gccgcgaccg
     1201 acaagtccgg cgacccggtc cagggcaggc ctggcgacga cggcttcggc acgatcgcgc
     1261 agggcttcac cgaggcctcg aacgtcaacc tgacggacga gctcgtcaac ctgatcgtcg
     1321 cgcagcgcgc ctaccaggtg aacgcgaagg tgatccaggc ctcggacgac atgctcaaca
     1381 tcaccaacaa cctgcggcga tagagggaat cgtgttgcgc cccgtccccg ccattgccgt
     1441 gcttcttgcg ctcgtcgcaa cgggtgcccg cggcgccgat cccgtcgtcg aggccgcgcg
     1501 cgcgcacctg gagcgcgcgc tcgcgcctgc gcacgagcag gtaatcgtcg agccgctccc
     1561 ggggcgccag gcggcgccgc tgcctgcggg cggcgtgtcg ctcgagacgc gcggctgcgg
     1621 gcgcgagaca cccgcgcggc gcatgtgcgt gtgggtcgac gtcatggtcg acggcgcgcg
     1681 ccgaagctcg gtcgcggtgt ggttccgggt ggaggcgaag cgcaaggtcc tggtcgccac
     1741 cgacccggtg ccggcggacg ccgagttcga cccgcgcgcc ttcgcgctcg agctgcgcga
     1801 tgtcgccgcg cttcccggcg ccccgctcgc gaccgccgcg cacgcgggcc gcctgcgcgt
     1861 gaagcggccg ctcgagcccg ggcaggtcgt gctcgagcgc gacgtgacgc ctattcccgc
     1921 ggtcgcgcgc aaccaggaag tgacgatccg gctccagacg ggcgggatcc tgatcgaggc
     1981 gcccggcgtc gcgctctcgg aggggcgggt cggcgcgacg gtgcgcgtgc gcaacccgag
     2041 gagcggggag atctaccagg cccgggtcgt cgacgatggc gtgctcgcgg tcacactgag
     2101 ataggagaac agccgatgcc aactcgtgcg tgcctcgccg tcgtcctggc cgtcctcgcg
     2161 gccgacgccg cgctccccgc agcggcagag agcctgtacc agccggggca gtaccagccg
     2221 ctcgtcgcgg accggcgcgc gtaccgcccg ggtgacgtgc tgaccgtcca ggtatacgag
     2281 aattcgagcg cccagagcac cgccgacacc acgacgaaga aggacggctc gctcaacctc
     2341 tcgctgaaga cgccggcgat caacaaggcg gccggcgcgc agctctccga tgatttcaac
     2401 ggaaccggca agacgcagcg caccgggaag ctgctcgcgc agttcaccgt cgtcgtgcaa
     2461 ggtgtcgacg cgaacggctt gctgatcatc aagggccagc agctgatcga cgtgaacgac
     2521 gagaagcagg agatccggct cgagggcaag gtgcggccga tcgacatcgc cgacaacaac
     2581 accatcctct cgacgcgtat cgccgacgcc aggatcagct acatgggcga cggcgtgctg
     2641 ggcgagcacc agcggcccgg catcctgacc cgcatcctct cgtggctggg gatcctgtga
     2701 aggcggcgct cgtgccgctg ctggccgccg ttctcgccgc gccggcgagc gcgcagccgg
     2761 gcgacggcgt gcgcatcaaa gatctcggcc gcatcgacgg cgtgcgcgag aaccagctcg
     2821 tcggctacgg catcgtcacc ggcctcgccg gatcgggcga ttcgtcgcgc agccgcgcga
     2881 cgcgccagtc gatcgcgaac ctgctgtcgc agttcgggct gtacgttccg aacgaccagg
     2941 tgcagagccg caac
//
LOCUS       DATDMW010000369         6587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1351982, whole genome shotgun sequence.
ACCESSION   DATDMW010000369 DATDMW010000000
VERSION     DATDMW010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351982"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..389
                     /locus_tag="VI319_15445"
     CDS             87..389
                     /locus_tag="VI319_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY6865289.1"
                     /translation="MPAYWVARSRIDNPVEYKKYTDRVPGIIAKFGGKVLARGGRYRI
                     LEGPQKFQRFVVIEFPTLERGIECWESKEYQEASAFRRNGGGEVELVMVEGGDATK"
     gene            complement(394..1008)
                     /locus_tag="VI319_15450"
     CDS             complement(394..1008)
                     /locus_tag="VI319_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEY6865290.1"
                     /translation="MRAYDGTDPPAKTARAWPRSAWLADLRRLYQSYDGSTLRRIALT
                     STAPGAHAVTAFRFGHWLLGQPKLVRLALDPVYHVLDTGVRILWGIEISRHAVVGPGL
                     IIGGSHFGGITVSGRTVLGRDCTISQQVTIGMSGQGDRAGVPIIGDCAYIGPGARVFG
                     KIVVGNNVKIGANAVVYKDIPDRAIVVLDPGFRIVSYKGNRVGT"
     gene            1142..1510
                     /gene="ssrA"
                     /locus_tag="VI319_15455"
     tmRNA           1142..1510
                     /gene="ssrA"
                     /locus_tag="VI319_15455"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1530..2840
                     /locus_tag="VI319_15460"
     CDS             1530..2840
                     /locus_tag="VI319_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY6865291.1"
                     /translation="MASVFHITRISWRDLAMTVVPFVVLLAIALWIASRFVHPAPPDT
                     ITISSGPEGSSYALVAERYRKILARQGVTLKVLPSRGSLENLQRLADPKTQVDIGFVQ
                     GGLAGQADTGDLVSLGSIFYTPVYLFYRAPKPMTRLSELSGRRVSTGREGSGTHVLAT
                     ALLKANGIDGKEGTRLVNLEGKAAEEALQKKEIDAAFLMGDSAAFANVRDLLHSEGVR
                     FFDFKQADAYVGRFRYLTKIGVPAGAFDLGKNLPPENLTLVSPTVELIARSDLHPALS
                     DMLIEAAREVHGRANLYQKAGEFPAPLEHEYRVSDDAKRYYQSGKTFAYRHLPFWLAS
                     LVDRIVVVLVPLAVLLIPGFKLLPWLYRWRVNQRIYRRYAELMALERAAFAKTTPEER
                     ADLLRRLDRIEENVIKLKVPGSFAEQGYVLRQHIGFVRARLAQG"
     gene            2840..3865
                     /locus_tag="VI319_15465"
     CDS             2840..3865
                     /locus_tag="VI319_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865292.1"
                     /translation="MSALHLVGSIPLDTPRDVFETFGRTLGPHLACCPDGEVGLRRHW
                     ISRVHFQVFAIHPDIETIQRPALEDGVERLMPRSAADSWRFRVRDGVEQIRFGEPGWR
                     LGFAREAVNSYFVFRTLKEKGVLPAHLRFQVSMPMVNSAAPPRLFPDERSCALVRVGY
                     ARALAAELEKIVEKIPQRELAIQWDCSTELQEAYHGRLDEQVPQVRLSESIPGEVAVG
                     FHLCFGTLGGWPRFTPKDLGAAVELANAFVAAAGRRVDWIHIPVLDTGADAFFAPLRG
                     LDARGARIYLGMVHNMERFPARLAAARKHLNDFGVGAYCGFGRLPPDDLPRVLEEHRR
                     AAELLSR"
     gene            complement(3862..4815)
                     /locus_tag="VI319_15470"
     CDS             complement(3862..4815)
                     /locus_tag="VI319_15470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY6865293.1"
                     /translation="MPKLVHWIVQGICAGALALALAAPALAQPAAQLVVFGDSLSDPG
                     NAFALVGGTNTPPDYSVDFFFVPSQPYARGGHHFTNGDTWIELLARARGMNANAQPAF
                     RSNTAGAANYAIGGARARDDGSATSLSRQVDAFLGDTGGAASGGALYVIEVGGNDIRD
                     IAQTRDPSLATAALAAIQSNIARLYGAGARTFLVWNAPKVAATPAIRMADAALPGTAA
                     AVDFVTLQYNAGLAQLLSGLEAALPGVRFIRYDVYAGLAAIVASPAAFGLSNVTDPCI
                     RPNLAPFFCQAPRAYLFWDGIHPTEAGHAIIADQVGKLLGP"
     gene            4963..5277
                     /locus_tag="VI319_15475"
     CDS             4963..5277
                     /locus_tag="VI319_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865294.1"
                     /translation="MLKPMIVAILGALALTGLPAYAQSDVQKDTQDIRQDRRDLRQDK
                     RERRQDLRQARKDAKAGDTQGAQAERREARQETRDVKQDRRDLRKDKRDRRRDRKAAA
                     KE"
     gene            5377..>6587
                     /locus_tag="VI319_15480"
     CDS             5377..>6587
                     /locus_tag="VI319_15480"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY6865295.1"
                     /translation="MIRSAVIALAVWLAAPAARAEPPAGADLRFLDRLTYGATTRDID
                     AYRRLGRDAWLREQLVYHGDDGLPSEAREAIAALRISRLNPEEAAAELRAMRTAARGK
                     SPAERADLQKRLRREFAALDLETFQRRTIRALASPYPLQEQLTWFWFNHFNVFQNKKQ
                     VRFLLPDYEERAIRPHVLGKFRDLLRAVLMHPAMMVYLDNAQNVRGAINENYARELME
                     LHTLGVDGGYTQADVQALARILTGAGVDLSPRPEGRRGDFVKVGLFAFHPGRHDAGAK
                     TFLGARFPGETGFSEIERALDLLCRHPATARFVSRKLAVYFLADDPPAEVIDRMAEAF
                     TRTDGDIARTLAALFSSPQFADPAATPRKFKDPVQWVYSSIRLLYPDAVPANSRALAG
                     ALAQLGEPVYG"
BASE COUNT         1032 a         2320 c         2235 g         1000 t
ORIGIN      
        1 gcggtgatcg cgcacccgcg cgaggtcctc tcgcacttgt catcctgagc gcagcgaagg
       61 atctgctttt cgccaaggag aacgaaatgc ccgcctactg ggtcgcaaga tcgcgcatcg
      121 acaacccggt cgagtacaag aagtacaccg accgcgtccc cgggatcatc gccaagttcg
      181 gcggcaaggt cctcgcgcgc ggcgggcgct accggatcct cgagggaccg cagaaattcc
      241 agcgcttcgt cgtgatcgag ttcccgacgc tcgagcgcgg catcgagtgc tgggagtcga
      301 aggagtacca ggaagcgtcc gcgttccgcc gcaacggcgg cggcgaggtc gagctggtga
      361 tggtcgaggg cggggacgcg acgaaatgag aagctacgtg ccgacgcggt ttcccttgta
      421 cgacacgatg cggaacccgg gatcgagcac gacgatcgcc cggtccggga tgtccttgta
      481 gacgaccgcg ttggcgccga tcttgacgtt gttgccgacg acgatcttgc cgaagacgcg
      541 cgcgccggga ccgatgtagg cgcaatcccc gatgatcggc actccggcgc ggtccccctg
      601 cccggacatt ccgatcgtca cctgctgcga gatggtgcag tcccttccga gcacggtcct
      661 tcccgacacc gtgatgccgc cgaaatgcga tccgccgatg atcagccccg gaccgactac
      721 ggcgtgccgc gagatctcga tgccccagag aatgcggacc ccagtgtcga gcacgtgata
      781 gaccgggtcg agcgcgagcc tcaccagctt cggctggccg aggagccagt gcccgaagcg
      841 gaacgcggtc acggcgtggg cgcccggcgc cgtcgaggtg agcgcgatcc ggcggagcgt
      901 gctgccatcg tagctctggt agagacgccg gaggtcggcg agccaggcgc tccgaggcca
      961 cgcccgcgcc gtcttggcgg gtgggtcggt gccgtcgtat gcgcgcatgg agtcatcatg
     1021 ggcggcgcat agctcatccg gaacgggagg gtccatagga caattggcct atgctcggga
     1081 ttgaaacatc ggggccgccg ccccacctgt gggataatcg cagtggcttc aacgcgattc
     1141 cgggggcgac ctggtctcga cgtgggttgc gaagcagcgc agggcatgcc gaggaccaga
     1201 agacctcgtt aatccatctg gaacatctat acacgccaac gacgagcgtt ttgctctcgc
     1261 ggcctaatac ccgcgagacg ctgcactagc ctgctgatgg gctaggcctc cgcaaggagg
     1321 tgcagcgcca ttcacatcag cccctctttc cggtttggcc gcttggccgg aaggatccgt
     1381 cgaagcggct agccgaacgc cagcctgccg gccggcgggc gcgaggcgaa gctcaaacga
     1441 ccaggctaag catgtagtcc tgactgtgga ccgcttacgg acgcgggttc gattcccgcc
     1501 gcctccacca cttacacaat ctcgcgggct tggcttccgt ctttcacatc acgcggatct
     1561 cctggcgcga cctggcgatg acggtcgtgc cgttcgtcgt tctgctggca atcgcgctct
     1621 ggatcgcctc gcgcttcgtg cacccggcgc cgcccgacac gatcacgatc agcagcggcc
     1681 cggaaggcag cagctacgcc ctggtcgccg agcgctaccg caagatcctc gcgcgccagg
     1741 gcgtgacgct caaggtcctg ccctcgcggg gctcgctcga gaacctgcag cgcctcgccg
     1801 atccgaagac gcaggtcgac atcgggttcg tccagggcgg gctcgccggc caggcggaca
     1861 cgggcgatct cgtctcgctc ggcagcatct tctacacgcc cgtctatctt ttctaccgtg
     1921 cgccgaagcc catgacccgg ctctccgagc tctccggcag gcgcgtctcg accggccgcg
     1981 aaggcagcgg cacccacgtg ctcgccaccg cgctgctgaa ggcgaacggc atcgacggca
     2041 aggaagggac ccggctggtc aacctcgagg ggaaggcggc cgaggaggcg ctgcaaaaga
     2101 aagagatcga cgctgcgttc ctcatgggcg actcggccgc gttcgccaac gtccgcgacc
     2161 tgctccacag cgagggtgtc cgcttcttcg acttcaagca ggccgacgcc tacgtgggcc
     2221 gcttccgcta cctgacgaag atcggcgttc ccgccggcgc cttcgacctc ggcaagaacc
     2281 tgccgcccga gaacctgacg ctcgtctcgc cgacggtcga gctgatcgcg cgctccgatc
     2341 tgcatcccgc gctctcggac atgctgatcg aagccgcgcg cgaggtgcac gggcgcgcca
     2401 acctgtacca gaaggcgggc gagttcccgg cgccgctcga gcacgaatac cgcgtcagcg
     2461 acgacgcgaa gcggtactac cagtcgggca agacgttcgc ctaccggcac ctgcccttct
     2521 ggctggcgag cctggtcgac cgcatcgtcg tcgtgctcgt gccgctcgcc gtgctcctga
     2581 tcccggggtt caagctcctg ccctggctct accgctggcg cgtcaaccag cgcatctacc
     2641 ggcgctacgc cgagctgatg gcgctcgagc gcgcggcgtt cgcgaagacc acacccgagg
     2701 agcgcgccga cctcctacgg cggctcgacc ggatcgagga gaacgtcatc aagctcaagg
     2761 tccccggctc tttcgcggag cagggctacg tcctgcgcca acacatcggg ttcgtgcgcg
     2821 cgcggctggc gcagggctga tgagcgctct ccacctcgtc ggctccatcc ctctcgacac
     2881 gcccagggac gtcttcgaaa ccttcggccg cacgctcggc cctcacctcg cctgctgtcc
     2941 cgacggcgag gtcggcctgc gccggcactg gatctcgcgc gtgcacttcc aggtgttcgc
     3001 gatccatccc gacatcgaga ccatccagcg ccccgcgctc gaggacggcg tcgagcgcct
     3061 gatgccgcgc agcgcggccg acagctggcg cttccgcgtg cgcgatgggg tggagcaaat
     3121 ccgcttcggc gagccgggat ggcggctcgg gttcgcacgc gaggcggtga attcgtattt
     3181 cgtgttccgc acgctgaagg agaaaggcgt ccttccggcc catctccggt tccaggtctc
     3241 gatgccgatg gtcaacagcg cggcgccgcc ccgcctcttc ccggacgagc gctcgtgcgc
     3301 gctggtgcgc gtgggctacg cgcgcgcgct cgcggcggag ctcgagaaga tcgtcgagaa
     3361 gatcccgcag cgcgagctcg cgatccagtg ggactgctcg accgagctgc aggaggcgta
     3421 ccacgggcgc ctcgacgagc aggtgccgca ggtgcggctt tccgaatcca ttcccgggga
     3481 ggtcgccgtc ggcttccatc tctgcttcgg cacgctcggc ggctggccgc gcttcacgcc
     3541 caaggatctc ggcgcggcgg tcgagctcgc gaacgccttc gttgccgcgg cgggacgccg
     3601 cgtcgactgg atccacatcc ccgtgctcga cacgggcgcg gacgcgttct tcgcgccttt
     3661 gcgcggcctc gatgcccgcg gcgcgcggat ctacctcggc atggtgcaca acatggagcg
     3721 cttccccgcg cgccttgcgg ctgcgaggaa gcacctgaac gacttcgggg tcggcgccta
     3781 ctgcggcttc gggcgcctgc cgcccgacga tctcccgcgc gtgctcgagg agcaccggcg
     3841 cgcggccgag ctgctctcgc gctaaggtcc gagcagcttc ccgacctggt ccgcgatgat
     3901 cgcgtgcccc gcctcggtcg ggtggatgcc gtcccagaag agatacgcgc gcggcgcctg
     3961 gcagaagaac ggcgcgaggt tcggccggat gcacggatcg gtgacgttcg acagcccgaa
     4021 cgccgccggg ctcgcgacga tcgccgcgag gccggcgtag acgtcgtagc ggatgaagcg
     4081 cacgcccggg agcgcggcct cgaggcccga gagcagttgc gcgaggccgg cgttgtactg
     4141 cagcgtgacg aagtcgaccg ccgcggccgt gcccgggagc gcggcgtccg ccatccggat
     4201 cgccggcgtc gcggcgacct tgggcgcgtt ccacaccagg aacgtcctcg cgcccgcgcc
     4261 gtacagccgt gcgatgttcg actggatcgc ggcgagcgcc gcggtggcga ggctcgggtc
     4321 ccgcgtctgc gcgatgtcgc ggatgtcgtt gccgccgacc tcgatcacgt agagcgcgcc
     4381 gcccgaagcg gctccgcccg tgtcgccgag gaacgcgtcg acctggcggc tcaggctcgt
     4441 ggcgctgccg tcgtcgcgcg cccgcgcgcc gccgatcgcg tagttcgcgg cgccggcggt
     4501 gttgctgcgg aacgccggct gcgcgttcgc gttcatcccg cgcgcgcgcg cgagcagctc
     4561 gatccacgtg tcgccgttcg taaagtgatg tccgccgcgc gcgtacggct ggctcgggac
     4621 gaagaagaag tccaccgagt agtcgggcgg cgtgttcgta ccgccgacca gcgcgaacgc
     4681 gttccccgga tccgacaggc tgtcgccgaa cacgaccagt tgcgccgcgg gctgggcgag
     4741 cgccggggcg gcgagcgcca gcgcgagcgc gccggcacag attccttgca cgatccagtg
     4801 tacgagcttg ggcatggtgt ctctcctccg tggggcaatc attgtcgctc acaatcgtgt
     4861 cggagacgac aggtgacgtc aacggagcgc gcgcacggac gtttctggag tggtgcggcg
     4921 cgcaatgcgc cgattaccca acgaacccaa ggaggcgtca acatgctcaa gccgatgatc
     4981 gtcgccattc tcggcgcctt agccctgacg gggctgccgg cgtacgcgca gagcgacgtg
     5041 caaaaggaca cgcaggacat ccgccaggac cgccgcgacc tgcgccagga caagcgcgag
     5101 cgccggcagg acctgcgcca ggcgcgcaag gacgcgaagg ccggcgacac gcagggcgcg
     5161 caagccgagc gccgggaggc gcgccaggag acgcgcgacg tcaagcagga ccgccgcgat
     5221 ctgcgcaagg acaagcggga ccgtcgccgc gaccgtaagg cggccgcgaa ggagtaagcc
     5281 gggggagcgg ccccggggcg cgagtcccgg gcgtcgcgat acaaaatgtt tcagcagtcg
     5341 acggcgaact gcggtacatc gttggctgga agcaccatga ttcgatccgc cgtcatcgcg
     5401 ctcgccgtct ggctcgccgc gcccgccgcg cgcgccgagc cgcccgcggg ggcggacctg
     5461 cggttcctcg accgcctgac ctacggcgcg accacccgcg acatcgacgc ctatcgccgg
     5521 ctcgggcgcg acgcgtggct gcgcgagcag ctcgtctacc acggagacga cggcctgccg
     5581 tccgaggcgc gcgaagcgat cgcggccctg cggatctcgc gcttgaatcc cgaggaagcc
     5641 gcggccgagc tgcgcgccat gcggacggcg gcgcgcggga agtcgcccgc cgaacgagcc
     5701 gacctgcaga agcgcctgcg gcgcgagttc gccgcgctcg acctcgagac gttccagaga
     5761 cgcaccatcc gcgcgctcgc ctcgccgtat ccgctgcagg agcagctcac ctggttctgg
     5821 ttcaaccact tcaacgtgtt ccagaacaag aagcaggtgc gctttctcct gcccgactac
     5881 gaggagcgcg cgatccggcc gcacgtcctc ggcaagttcc gcgatctgct gcgcgcggtg
     5941 ctgatgcacc cggcgatgat ggtgtacctc gacaacgccc agaacgtgcg cggcgccatc
     6001 aacgagaact atgcgcgcga gctgatggag ctgcacacgc tcggcgtgga cggcggctac
     6061 acgcaggccg acgtgcaggc gctcgcacgc atactcaccg gcgccggcgt cgacctttcg
     6121 ccgcgtcccg agggtcgccg cggcgacttc gtgaaggtcg gcctgttcgc gttccatccc
     6181 gggcgtcacg acgcgggcgc gaagaccttc ctcggggccc ggtttcccgg cgagacggga
     6241 ttctccgaga tcgagcgcgc gctcgacctg ctctgccgcc atcccgcgac cgcgcgcttc
     6301 gtctcgcgca agctcgcggt ctacttcctc gccgacgatc cgccggcgga agtgatcgac
     6361 cgcatggccg aagcgttcac gcgcaccgat ggcgacatcg cgcggacgct cgcggccctt
     6421 ttctcgtcgc cgcaattcgc cgacccggcg gccacccctc gcaagttcaa ggacccggtg
     6481 cagtgggtgt actcgtcgat ccggctgctc taccccgacg ccgtgcccgc gaattcgcga
     6541 gcgctcgcgg gcgcgctcgc gcagctcggc gagccggtct acggaca
//
LOCUS       DATDMW010000370         5215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_480749, whole genome shotgun sequence.
ACCESSION   DATDMW010000370 DATDMW010000000
VERSION     DATDMW010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480749"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="VI319_15485"
     CDS             complement(<1..1555)
                     /locus_tag="VI319_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865296.1"
                     /translation="MTSSTAARRSRRQAAKLAKLTPPNPEGLVPRHRLFRALDRVSGG
                     GAAWISGPGGAGKTSLVASWLAARGQRVVWYQVDPGDRDPATLFHYLGIAAQELGRAP
                     QRPLPHFTPEYHGGLREFTRRHFQALFSRLAVPCAVVFDNCHAAGGDAPLEQILAWAM
                     SEVPARVALVCISRSAPGEALAGRLADAALVRIGADDLRLDEREARAIARSRGYRSSA
                     GSAVLGIVRGWAAGLVLTLRAKAQGIAPALGAGDARHPLFEYFAGEIFAAAGAETRGF
                     LMHAALLPVMSPHLVERLTGRAGAGQVLEDLHRGHFFTERRISAGGDSSYEFHPLFRD
                     FLLSRARETFGAEGFSQRCRQAAALLAPAGQVEAAVALLAEGRDWPALARLIGEQART
                     LMQQGRWRTLIGWIASVPADVVAESAWLTYWRGACLCFTDAAAARDALEAAYGMFEAS
                     GEGFGAALACTGVIQTYAWGAMTPALGWITRLEEALARILSPPIELELAVIDCMLGVL
                     FAQPLHPMIR"
     gene            1759..2253
                     /locus_tag="VI319_15490"
     CDS             1759..2253
                     /locus_tag="VI319_15490"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEY6865297.1"
                     /translation="MKPSELLIAAAMAVLAGAALAEPMNLKPGLWETTEKVQAGAAVA
                     GGLGRLVRGADKLPIGADTSRGVTTKRCITAEDLKHDNWFAKDKDPSCKFSVTTSSKN
                     AFVASRQCGTKVSHQADVDIRSPDPEHWTGSVKSKVTSGGKEETTTLELSGKWLAADC
                     GAKK"
     gene            2250..2999
                     /locus_tag="VI319_15495"
     CDS             2250..2999
                     /locus_tag="VI319_15495"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEY6865298.1"
                     /translation="MRTPAPVLSAAMVALAAVLATGCADLLVGDSAPTQNTGATSQAL
                     QKLPRRPGERVPVTLYEFRSEVPEVNGRAATDMFITALVKSGQFSVQERSRVNQGVIR
                     EKQMNAAGQTTGNSGQVPLREARYVFEGTVSEANGSVKSNRTGFNIGGAQIGGGTNTD
                     TIGVDVRIVEVATGDIVDAINVRKEMKSATTSASGFNTLVTNVRAQKGKETSTLMPDV
                     THESTRKESVDAALRACIESAVAELSKRFQQ"
     gene            3004..3348
                     /locus_tag="VI319_15500"
     CDS             3004..3348
                     /locus_tag="VI319_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865299.1"
                     /translation="MRVAVFVVAAVMLAGAGPAAAEDATRAPIDWRPVWLDPPRTDPL
                     LLVAPPYAATATPRWRPAAARAPGAAMLPPRAPSVLLERRPDAAQGCAVFDGCLPYVP
                     VVPITENPGRWP"
     gene            3345..5051
                     /locus_tag="VI319_15505"
     CDS             3345..5051
                     /locus_tag="VI319_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865300.1"
                     /translation="MRKTLAACAALLAAASAGAALAQTRAASPFGTGPQESQPPADWR
                     AGQRNSTVAPAQTPPNPAIPPNPCMTSKVQGATPPNPCTPPVSARTAAKLSAAPVIRL
                     APAQPAFMLAGVCQAGKPVATLRVVATNAGTVSVPPSAPAMRITAIDDTPLAWAGSVK
                     LPALAAGASATVAIPLLPPSRPDAVAGKHRFSVMAVPLGIAAASPIEMSIPAGFCNRL
                     TARDPKRTVLSMAHGAPAMTTAMNNTTPRGASKFAPKVDLALPAPSALTSTVDEKVCN
                     THGGFGGGLACAALLPQGRLALVWSWSGASADGYKVYRVDGGQHTYVGRQANGKDATL
                     YIVDPVPAGGYTGKCYAVTAFRGTEESAPSGATCAGGGNVVSTVTLAPLDVRSLEHYR
                     WVPRGLGGGGPYENIVDRGGVRAGYSYATQKYFSGDGYADSIYETALHFDSSAFEHKT
                     VRAARLRLRVDTSNVGTDRHEDHVLSCATSIQIGREYWWDHRDRPVMINAVAGAHPGV
                     YNGPDIALDVTDIVRGWAQSPGTNYGLVLAGDKTAVDVFVDDACETAFVPELTRLEVE
                     FY"
BASE COUNT          823 a         1826 c         1918 g          648 t
ORIGIN      
        1 tccgtatcat cggatggagc ggctgcgcga acagcacgcc gagcatgcag tcgatcaccg
       61 cgagctcgag ctcgatcggc ggcgacagga tgcgcgcgag cgcctcctcg aggcgggtga
      121 tccacccgag ggccggggtc atcgcacccc aggcgtaagt ctggatgacc ccggtgcacg
      181 cgagcgcggc gccgaagcct tcgcccgacg cctcgaacat gccgtacgcg gcctcgagcg
      241 cgtcgcgcgc ggccgcggcg tcggtgaagc agaggcacgc gccgcgccag tacgtcagcc
      301 acgccgactc cgccaccacg tccgcaggga cgctcgcgat ccagccgatg agggtccgcc
      361 agcggccctg ctgcatgagg gtgcgcgcct gctcgccgat caggcgcgcg agcgccggcc
      421 agtcgcggcc ctcggcgagg agggccaccg cggcctcgac ctggccggcc ggcgcgagca
      481 gcgccgcggc ctgccggcag cgctgcgaga agccctcggc gccgaacgtc tcgcgggcgc
      541 gcgagagcag gaagtcgcgg aacagcgggt ggaactcgta gctcgaatcg ccgcccgccg
      601 agatgcgccg ctcggtgaag aagtggccgc ggtgcaggtc ctcgagcacc tgcccggcgc
      661 cggcgcggcc ggtgaggcgc tcgaccaggt gcggcgacat gacgggcagc agcgccgcgt
      721 gcatgaggaa gccgcgggtc tcggcgccgg cggccgcgaa gatctcgccc gcgaagtact
      781 cgaacagcgg atggcgcgcg tcgcccgccc cgagcgccgg ggcgatgccc tgcgctttcg
      841 cgcgaagcgt gagcaccagg cccgcggccc atccgcggac gatgccgagg acggcggagc
      901 ccgcgctcga acggtagccg cgcgagcgcg cgatggcgcg ggcctcgcgc tcgtcgaggc
      961 gcaggtcgtc cgcgccgatg cgcacgagcg ccgcgtccgc gaggcggccc gcgagcgcct
     1021 cgcccggagc gctgcggctg atgcagacga gcgcgacccg cgccggcacc tcgctcatcg
     1081 cccacgcgag gatctgctcg agcggagcat cgccgcccgc ggcgtggcag ttgtcgaaga
     1141 cgaccgcgca cgggacggcg agccgcgaga acagcgcctg gaagtggcgc cgggtgaatt
     1201 cgcgcaggcc gccgtgatat tccggcgtga agtgcggcag cgggcgctgc ggggcgcggc
     1261 ccagctcctg cgcggcgatg ccgagatagt ggaacagcgt ggcgggatcg cggtcgcccg
     1321 ggtcgacctg gtaccagacg acgcgctgcc cgcgcgcggc gagccagctc gcgacgagcg
     1381 acgtcttgcc ggcgccgccc ggcccggaga tccacgccgc gccgccgccg ctcacgcggt
     1441 cgagcgccct gaacagccgg tgacgcggca ccagcccttc ggggttcggg ggcgtgagct
     1501 tggcgagctt cgcggcctgc cggcgagagc gcctagccgc ggtcgaggac gtcatgcatc
     1561 ggagctgttc ttatggttgt tgaagccgct gcgaaaaacg gtccgcctcc cccgcggacc
     1621 cagcgataac actgctcccc gcagggatta ttcctccgga gtctgacaac gcgctgtcat
     1681 tgcgatcggt aagtcatccg taagtggcgc gcgtctaggg tttcgggcac gaaaaccaag
     1741 caaaatcaag gagaggggat gaaaccgtcc gagttgttga tcgccgccgc aatggcggtg
     1801 ctggccggcg cggcgctggc cgagcccatg aacctcaagc ccggcctgtg ggagacgacc
     1861 gagaaggtcc aggcgggcgc tgcagtcgcc ggcggcctgg ggcgcctggt gaggggcgcg
     1921 gacaagctcc ccatcggcgc ggacacctcg cgcggcgtga cgaccaagcg ctgcatcacc
     1981 gcggaggatc tgaagcacga caactggttc gcgaaggaca aggacccgag ctgcaaattc
     2041 agcgtcacca cgagctcgaa gaacgcgttc gtcgcgagcc gccagtgcgg cacgaaggta
     2101 tcgcaccagg cggacgtcga catccgctcg ccggaccccg agcactggac cggcagcgtc
     2161 aagtcgaagg tgacgagcgg cggcaaggaa gagacgacga cgctcgagct gtcgggcaaa
     2221 tggctcgccg cggactgcgg cgcgaagaaa tgaggacgcc cgcgcccgtg ctgtcggcgg
     2281 cgatggtcgc gctcgcggcc gtgctcgcga ccggctgcgc cgacctgctg gtcggcgact
     2341 ccgcgccgac gcagaacacc ggggcgacct cgcaggcgct gcagaagctg ccgcggcgcc
     2401 ccggcgagcg cgtgccggtg accctgtacg agttccgctc cgaggtcccc gaggtgaacg
     2461 gccgcgccgc caccgacatg ttcatcaccg cgctggtgaa gtcgggacag ttctcggtgc
     2521 aggagcgctc gcgcgtcaac cagggcgtga tccgcgagaa gcagatgaac gccgcgggcc
     2581 agacgaccgg caactcgggc caggtgccgc tgcgcgaggc gcgctacgtg ttcgagggca
     2641 cggtgtccga ggcgaacggc tcggtgaagt cgaaccgcac cgggttcaac atcggcggcg
     2701 cgcagatcgg cggcggcacg aacaccgaca cgatcggggt ggacgtgcgt atcgtcgagg
     2761 tcgccaccgg cgacatcgtc gacgcgatca acgtgcgcaa ggagatgaag agcgcgacca
     2821 cgtcggcctc gggcttcaac accctggtca cgaacgtgcg cgcgcagaag ggcaaggaga
     2881 cttcgacgct catgcccgat gtcacgcacg agagcacgcg caaggaaagc gtggacgcgg
     2941 cgctgcgggc ctgcatcgag tcggcggttg ccgagctgag caagcggttt cagcaataaa
     3001 ggcgtgcgcg tcgcggtctt cgtcgtcgcg gccgtgatgc ttgcaggcgc tgggccggcg
     3061 gccgcggaag acgccacgcg cgcgccgatc gactggcggc ccgtctggct ggatccgccg
     3121 cgaaccgatc cgctgctgct cgtcgcgccc ccttacgcgg cgacggcgac gccgcggtgg
     3181 cgtccggccg ccgcgcgcgc gcccggcgcg gcgatgctgc cgccgcgcgc gccgagcgtg
     3241 ctgctcgagc ggcgccccga cgcggcgcag gggtgcgcgg tcttcgacgg ctgcctgccc
     3301 tacgtcccgg tcgtaccgat caccgaaaac ccagggagat ggccatgagg aagacattgg
     3361 cggcgtgcgc cgcattgctc gcagcggcgt ccgccggagc cgcgctcgcg cagacccgtg
     3421 ccgcttcgcc gttcggcacg gggccgcagg agtcgcagcc gccggcggat tggcgcgcgg
     3481 gccagcgcaa cagcacggtc gccccggccc agacgccgcc gaatccggcc attccgccga
     3541 atccgtgcat gacgagcaag gtgcagggcg cgaccccgcc gaatccgtgc acgccgccgg
     3601 tctcggcgcg aaccgcggcg aagctgagcg ccgcccccgt gatccgcctc gcgcccgcgc
     3661 agccggcgtt catgctcgcg ggcgtctgcc aggcgggcaa gccggtggcg actctgcgcg
     3721 tggtcgcgac caacgccggc accgtgtcgg tgccgccgtc ggcgccggcg atgcgcatca
     3781 cggcgatcga cgacacgccg ctcgcctggg cgggatcggt caagctgccc gctctcgccg
     3841 cgggagcgtc cgcgaccgtc gcgatcccgc tgctgccgcc gtcgcgcccg gacgcggttg
     3901 ccgggaagca ccggttcagc gtcatggcgg tgccgctcgg catcgcggcg gcgtccccga
     3961 tcgagatgag cattcccgcg ggattctgca atcgcctcac cgcgcgcgat ccgaagcgca
     4021 cggttctctc gatggcgcac ggcgccccgg cgatgaccac cgcgatgaac aacaccaccc
     4081 cgcggggcgc gagcaagttc gcgcccaagg tcgatctcgc gctgccggcg ccgagcgcgc
     4141 tcaccagcac cgtggacgag aaagtgtgca acacccacgg cggcttcggc ggcggactcg
     4201 cgtgcgcggc gctgctgccc cagggccggc tcgcgctggt gtggagctgg tcgggcgcgt
     4261 cggcggacgg ctacaaggtc taccgggtcg acggcggtca gcacacctac gtcggccggc
     4321 aggcgaacgg caaggacgcc acgctgtaca tcgtcgatcc cgtgcccgcc ggcggctaca
     4381 cgggcaagtg ctacgcggtg acggcattcc gcggaaccga ggagtcggcc ccgagcggag
     4441 ccacgtgcgc gggcggcggc aacgtggtct cgaccgttac gctggctccg ctggacgtgc
     4501 gctcgctcga gcattaccgc tgggtgccca gagggctcgg cggcggcggg ccgtatgaaa
     4561 acatcgtcga ccggggaggg gtgcgtgccg gttattccta cgcgacgcag aagtacttct
     4621 cgggcgacgg ctacgccgac tcgatctacg agacggcgct gcacttcgac tcgagcgcgt
     4681 tcgagcacaa gacggtccgc gcggcgcggc tgaggctgcg cgtggacacg agcaacgtcg
     4741 gcacggaccg gcacgaggac cacgtgctgt cttgcgcgac gagcatccag atcggccgcg
     4801 aatactggtg ggaccaccgc gaccggccgg tcatgatcaa cgcggtcgcc ggcgcgcatc
     4861 cgggcgtgta caacggcccg gacatcgcgc tcgacgtgac cgacatcgtg cgcggctggg
     4921 cgcagagtcc gggaacgaat tacggactgg tcctcgccgg agacaagacc gccgtcgacg
     4981 tgttcgtcga cgacgcctgc gagaccgcgt tcgtgccgga gctgacgcgc ctcgaggtcg
     5041 agttctactg agacattgcg gaagggccac atgaaccatg ctgcactgaa aatggcgctc
     5101 gcggtgatcg cgagctttgc cgcggacgcg gcgttcgcgc aaggcgcgcc gagcccgatg
     5161 caacgcgggg tgggttcgcc cttcggcact ccgcagcaac agcagcagca gccaa
//
LOCUS       DATDMW010000371         2173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1353064, whole genome shotgun sequence.
ACCESSION   DATDMW010000371 DATDMW010000000
VERSION     DATDMW010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353064"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..323
                     /locus_tag="VI319_15510"
     CDS             27..323
                     /locus_tag="VI319_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY6865301.1"
                     /translation="MAKGYWVVTYRKINNQESFQAYAKLAAPAIIAAGGKYLIRGTPA
                     AVHEKGMKERVVVSEFPSLQAAIAAHDGPGYQAALKELKPGAVERDMRIVEGLE"
     gene            323..694
                     /locus_tag="VI319_15515"
     CDS             323..694
                     /locus_tag="VI319_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEY6865302.1"
                     /translation="MFRGILGEDRIHPAVREKIARHGADIVREVEAAVAANDIVVVGM
                     RRNPFPRKARALLDAAGIPYQYLEYGGYFSPKDYRQRLPLKMWTGWPTFPMVFVNGTF
                     IGGFTDLEKLVQSGELKKLSS"
     gene            717..1226
                     /locus_tag="VI319_15520"
     CDS             717..1226
                     /locus_tag="VI319_15520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEY6865303.1"
                     /translation="MVLRAVLDTNVWLDWLVFDDPCVAPIREAHEAGRLEIVIAAEGE
                     AELARVLAYERGRHTRDAEAQAACLAECRKIARRIEAGLHEAQRARLPVCRDRDDQQF
                     LEAALASGADFLITKDRALLELALRQAQRERLQAQGERGLPFAIVAPADFAALTTPRA
                     ARSSGRSWW"
     gene            complement(1233..>2173)
                     /locus_tag="VI319_15525"
     CDS             complement(1233..>2173)
                     /locus_tag="VI319_15525"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6865304.1"
                     /translation="ALQVLAAAHAYAGRRLRSALLVGGMPYPKQLEQLRQPLDLAVAT
                     PGRLKDHLQRRSLDLGRVEMLVLDEADRMLDMGFAEDIDAIVAATPASRQTLLFSATL
                     DGVVGKLAARVTRSPRRIAVDAPAGPALRVDESAIFADGFDHKSRLLDALLRDAALTQ
                     ALVFTSTKRSAEELSQSLALRGFAAGALHGDMRQNERNRAMQRMHDGRTRVLVATDVA
                     ARGIDVPGISHVINFDAPRGAEDYVHRIGRTGRAGRAGTAVTLVGREDRALVRAIERR
                     RGSPMRIAVIPGLEQAKRRPEPSRPADRRLYSRVLP"
BASE COUNT          319 a          732 c          825 g          297 t
ORIGIN      
        1 gaactctccc cggaaaggaa gcgacgatgg ccaagggcta ttgggtggtc acttaccgca
       61 agatcaacaa ccaggaatcg ttccaggcat acgcgaagct cgcggcgccc gcgatcatcg
      121 cggcgggcgg caagtacctg atccgcggca cgccggccgc ggtgcacgag aagggcatga
      181 aggagcgcgt ggtggtgagc gagttcccga gcctgcaggc ggcgatcgcc gcgcacgacg
      241 ggccgggcta ccaggcggcg ctgaaggagc tgaagccggg cgcggtcgag cgcgacatgc
      301 gcatcgtcga gggcctggag tagtgttccg aggcatcctc ggggaggacc gcatccaccc
      361 ggcggtgcgc gagaagatcg cgcggcacgg cgccgacatc gtgcgcgagg tggaggcggc
      421 ggtcgcggcg aacgacatcg tcgtcgtcgg catgcgccgc aaccccttcc cgcgcaaggc
      481 gcgggcgctg ctcgacgcgg cgggcatccc gtaccagtac ctcgagtacg gcggctactt
      541 cagcccgaag gactaccggc agcgcctccc gctcaagatg tggaccggct ggccgacgtt
      601 cccgatggtg ttcgtgaacg ggacgttcat cggcgggttc acggatctcg agaagctggt
      661 gcagagcggc gagctcaaga agctgtcatc ctgagcggca gcgaaggatc tgctttttgg
      721 ttctccgcgc cgtcctcgac accaacgtct ggctcgactg gctggtcttc gacgatccct
      781 gcgtcgcgcc gatccgcgaa gcgcacgaag ccggccgcct cgagatcgtg atcgccgccg
      841 aaggcgaggc cgagctcgcg cgcgtgctcg cgtacgagcg cgggcggcac acgcgcgacg
      901 cggaggcgca ggcggcgtgc ctcgccgagt gcagaaagat cgcgcggcgg atcgaggcgg
      961 ggctgcacga agcgcagcgc gcgcggctcc cggtctgccg cgatcgcgac gatcagcagt
     1021 ttctcgaggc ggcgctcgcc tcgggggcgg atttcctgat cacgaaggac cgggcgctgc
     1081 tggaactcgc gcttcgacag gctcagcgcg aacggctaca ggctcagggc gagcgggggc
     1141 ttccgttcgc catcgtcgcg ccggcggact tcgccgcgct cacgacaccg cgggcagcac
     1201 gatcgtcagg ccggtcttgg tggtgatcgt cttcatggca ggaccctcga ataaagtctc
     1261 cggtccgcgg gccgggacgg ctcgggccgg cgcttcgcct gctccagccc cgggatgacg
     1321 gcgatgcgca tcgggctgcc gcggcggcgc tcgatcgcgc gcaccagggc gcggtcctcg
     1381 cggccgacga gcgtcacggc ggtcccggcg cgcccggcgc gcccggtgcg gccgatgcgg
     1441 tggacgtagt cctcggcgcc gcgcggcgcg tcgaagttga tgacatggct gatccccggc
     1501 acgtcgatgc cgcgcgcggc gacgtcggtc gcgaccagca cgcgcgtgcg gccgtcgtgc
     1561 atgcgctgca tcgcgcggtt gcgctcgttc tggcgcatgt cgccgtgcag cgcgccggcg
     1621 gcgaagccgc gcagcgcgag cgactgcgac agctcctcgg ccgagcgctt ggtcgacgtg
     1681 aagacgagcg cctgggtcag cgcggcgtcg cgcagcagcg cgtcgagcag ccggctcttg
     1741 tggtcgaagc cgtcggcgaa gatcgcgctc tcgtcgacgc gcagcgcggg cccggcgggc
     1801 gcgtccacgg cgatccgccg cggcgagcgc gtgacgcgcg cggcgagctt gccgacgacg
     1861 ccgtcgagcg tcgccgagaa cagcagcgtc tggcgcgagg ccggcgtggc ggcgacaatc
     1921 gcgtcgatgt cctcggcgaa acccatgtcg agcatgcggt cggcctcgtc gagcaccagc
     1981 atttcgacgc gcccgaggtc gaggctgcgg cgctggaggt ggtccttgag acggcccggc
     2041 gtcgcgaccg cgaggtcgag cggctggcgc aactgctcga gctgcttggg gtacggcatc
     2101 ccgccgacga ggagcgcgct gcgcaatcgg cggccggcgt acgcgtgcgc ggcggcgagc
     2161 acctgcagcg cga
//
LOCUS       DATDMW010000372        14510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_109195, whole genome shotgun sequence.
ACCESSION   DATDMW010000372 DATDMW010000000
VERSION     DATDMW010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14510
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109195"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VI319_15530"
     CDS             <1..352
                     /locus_tag="VI319_15530"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine-peptide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HEY6865305.1"
                     /translation="ALWAGVPLITCPGEHPVSRLGASLLHAIGLDELAARSVAEYESL
                     AIDIATHPDRLRAQKQKLASNRETHPLFDIARLVRNLERAYLEMQALQEAGRPPRSFD
                     IEDPAPFAAPAEFR"
     gene            362..772
                     /locus_tag="VI319_15535"
     CDS             362..772
                     /locus_tag="VI319_15535"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY6865306.1"
                     /translation="MSDQNRVTLPKGKDPLLRVVPMPHDANQHGDIFGGWIMAQVDIA
                     GGVLAARHARGRVATVAVNQFVFKQPVFIGDVVSFYADITRVGRTSITVNVEVYAQRN
                     PADVETVKVTEAVLTYVATDHNRKPRPVGPSGRG"
     gene            complement(773..1300)
                     /locus_tag="VI319_15540"
     CDS             complement(773..1300)
                     /locus_tag="VI319_15540"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6865307.1"
                     /translation="MGAGAGSLKLDLGCGASKQPGFVGVDVREFPGVDVVADLRGPWP
                     WDDGSVDEAFSSHFVEHLTAPERIHFVNELYRVLRTGAKATIVAPNWASARAYGDLTH
                     QWPPVSPFWFPYLNRAWRSQHAPHNDFYTCDFDVQYRFSVHEQFRDAEQTQRDFACLF
                     YVDGAQDIFAQLVKL"
     gene            complement(1308..2267)
                     /locus_tag="VI319_15545"
     CDS             complement(1308..2267)
                     /locus_tag="VI319_15545"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6865308.1"
                     /translation="MASNSRPFVIALEEHYYDAEVAKHFEGAEGRNPDLRRRLGDLGE
                     LRLREMDEAGIDVQVLSHGAPSTQRLDAETAIPLARRANDRLREAVREHPNRFRAFAA
                     LPTADPKAAADELERCVGKLGFVGAMIHGPSNGVFIDDKRFWPIFERAQELDVPIYIH
                     PATPMQAVIDAYYKDYIKDFPQIVSAAWGYTVETATMGVRLVLSGVCDKYPGTKIILG
                     HLGESLPFSAWRIDMCLSRGGKPSSFRDTFDSHFWITTSGNFSTPALMCSIMEMGVDR
                     VLFSVDYPFVLNPPGTKWLADLPLSLEDRVKICSGNAKRLLKI"
     gene            complement(2340..2732)
                     /locus_tag="VI319_15550"
     CDS             complement(2340..2732)
                     /locus_tag="VI319_15550"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY6865309.1"
                     /translation="MKAELMYLVWVTVVTALMWVPYILDRIAVWGLMDAVGYPASPKP
                     QSPWAVRMMKAHANAVENLVVFAALVLAAQQLGVSNGATATAAALYFWARLVHVLAYT
                     FAVPWVRTLAFAAGWIAQMIFAWQILAR"
     gene            complement(2749..3999)
                     /locus_tag="VI319_15555"
     CDS             complement(2749..3999)
                     /locus_tag="VI319_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6865310.1"
                     /translation="MDFAYSGKVRALEDKLRRFMDAHVYPSERRFHEEVAANRAKGDA
                     WVPTRLIEELKPKARAEGLWNLFLPRSPRAPQGLSNLEYAPLCEIMGRVHWAPEVFNC
                     SAPDTGNMETLERYGSEAQKREWLDPLLEGRIRSAFLMTEPDVASSDATNIRCRIERQ
                     GDHYVINGRKWWSSGAGDPRCKLFIVMGKTDPGAVRHAQQSMILVPRDAPGVKILRPL
                     TIFGYDDAPHGHMEVELRDVRVPAASLLLGEGRGFEIAQGRLGPGRIHHCMRIIGTAE
                     RALELMCRRTLGRVAFGKPIAEQGVTRERIAEARIMIDSARLLVLKAADMMDTVGNKA
                     ARAEIAMIKVLAPNVAERVIDWAIQAHGGAGMADDFPLARMYAWNRTLRLADGPDEVH
                     RNAIAKLELSKHIAVPGEKKQSAS"
     gene            complement(4012..5043)
                     /locus_tag="VI319_15560"
     CDS             complement(4012..5043)
                     /locus_tag="VI319_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY6865311.1"
                     /translation="MPVQERHRFDPAALERWLRLHVEGFSGPLSAEQYRGGQSNPTFR
                     LTAGGRAYVVRAKPGPAAKLLPSAHAVEREYRVMSALARAGIPVPRTLALCEDESVIG
                     RAFFVMECVEGRVLWEQSLPGMTRAERGATYDEMNRVIAALHRVDVAAVGLADYGKPG
                     NYFARQIGRWTKQYQASETEKIEAMDRLIDWLPAHVPPGEETAVVHGDYRMDNLIFHP
                     TEPRILAVLDWELSTLGHPLADFAYHCMSWHIPPGRFRGIGGLDLDALGIPREEAYVA
                     AYCARTGRAAVEHWDFYLAYNLFRIAAILQGIMKRALDGTAASEQALDAGRRARPLAE
                     LGWEYAQKA"
     gene            5262..5450
                     /locus_tag="VI319_15565"
     CDS             5262..5450
                     /locus_tag="VI319_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865312.1"
                     /translation="MVETIVFLMQMMSALVIVAGGVLTFSHVLEQEGVAAPSLDRAND
                     FEIDFRRVLSFARLAAQR"
     gene            5530..6714
                     /locus_tag="VI319_15570"
     CDS             5530..6714
                     /locus_tag="VI319_15570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6865313.1"
                     /translation="MPPSRTLTFFLNAGHAFDHLLMLIFPTVVLAMGSELGLGYAELL
                     PLSLGGFVAFGACSIPAGWLADRWSRYGMMLVFFFGIGAASLVTGLARTPAQIAVGLT
                     LVGVFGAIYHPVGIAMLVASQPRVGRVLGVNGVWGNAGVAFSALIAGALADTLGWRAA
                     FFVPGAAAIAAGAAFAFLVRDPGRGTPGQKTAPVRLSREDLVRVFAVLTVATACGGVI
                     FHATTVAMPKVFEERLGALTHTTFGIGSLVCGVYLVAALAQLCVGWWLDRRPLKSAFI
                     PVVALQVPLLALAGTAHDLAMVAVAVAMMFFVFGQIPINDAMIARYTAEEWRARAYAV
                     RYVVSFGGSALAVPLIAWLYRTSGDFRTLYFMLAALALAVLVAALIFPGEPKPARAGQ
                     AA"
     gene            complement(6784..7215)
                     /locus_tag="VI319_15575"
     CDS             complement(6784..7215)
                     /locus_tag="VI319_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY6865314.1"
                     /translation="MQMQFRSFVLASIAATALALSGCQTIKGWMGGGKVDLTGAQEVP
                     PVTTAAKGTANIKVAADKSVSGSVTTSGINGVAAHIHQGASGRNGPVIVPLTKKGDEW
                     LVPAGAKLTDAQYDAYKAGELYVNVHSPAHKGGEIRAQLKP"
     gene            complement(7291..8424)
                     /locus_tag="VI319_15580"
     CDS             complement(7291..8424)
                     /locus_tag="VI319_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEY6865315.1"
                     /translation="MGFAAMPFPPPAEAGTSLTDIDTPALVLDLDAFERNVARMADAM
                     KARGVRLRPHAKSHKCPEIAKRQIAAGAVGVCCQKVSEAEAMIAGGVRDVLVTNEIVG
                     AVKIERLAGLAHVARVAVLADDARNVAQLDAVAARRGVRLDVLIELDVGAGRCGVAPG
                     ESVLALGRVIAGARHLRFAGLHAYHGSAQHLRGPEERRAAIVAAASHALAARRALDGI
                     GLRCETVTGAGTGTFLLEAASGAYNEVQPGSYVFMDADYSRNRWDDDWPRFDQSLFLL
                     ASVMSAPASDRVVVDAGLKAASVDSGLPEVHAWPGLEYVRASDEHGVIRVSPDARVPA
                     LGERLLLVPGHCDPTVNLHDWLVCVRRGKVESVWPIAARGAVW"
     gene            8496..9473
                     /locus_tag="VI319_15585"
     CDS             8496..9473
                     /locus_tag="VI319_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY6865316.1"
                     /translation="MRVVEITQPGKPEVLKPAARALPQPKTGEVLVKVEAAGVNRPDV
                     LQRTGHYPVPPGASDIPGLEIAGRVAATGAGVSGWRVGDAVCALVQGGGYAEYCTAPA
                     LQCLPVPKGLSAIEAASLPETFFTVWSNVFDRARLAPGESFLVQGGSSGIGVTAIQMV
                     KAFGHTVFATAGSREKCDACEKLGADRAIDYKTEDFAAVVKEATGGRGVDVVLDMVGG
                     DYVDRELKCLADDGRIVLIAFLGGNRANLNLNEILRRRLVVTGSTLRPRPVEFKGAIA
                     AKLREKVWPLVEAGRIKPVIHETFPLDQAARAHELMESGRHIGKIVLVV"
     gene            9531..10307
                     /locus_tag="VI319_15590"
     CDS             9531..10307
                     /locus_tag="VI319_15590"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6865317.1"
                     /translation="MFAVYRELEGSTRLAAHVLRRFWVLRFPPVMEELVRQIYFTGAS
                     ATATIVVRGAFIGTLIIAYVIEVLEADAALAVKMLLWVVLREVGPLIAAVLVIQRSGT
                     AVATELALMKISGEVDSLRHLRIDPQDHLVVPRVLGIALSLAALTLYVQFIAVGGGMV
                     LSALVIDASFSELASNFFLLASPLDLAYSIVKSLLFGGAIGVIACYYGLNPPGRSINA
                     VPKAAINAVSQSTIAVMIINVVFAYLAYGVALFGLFTAKL"
     gene            10318..10950
                     /locus_tag="VI319_15595"
     CDS             10318..10950
                     /locus_tag="VI319_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865318.1"
                     /translation="MSELPTRVEEARQMALGYEAPPEGPSGRLWIPSGTGHRAAPVSQ
                     AFKQRLVDALLDLEPQPGARLAVLGADAGGLAARERQALRARIAFLPANGGMISNLNG
                     WENIVLPLGYRDAKRVNAAFPKVHALIERLGGHPRALLAKLPEEMTLYEKKLAGYVRI
                     LLEAPELVVAENLAGGLEPEDRRRTAAFPAAYHAERPDGVFVQLDDAPDD"
     gene            10966..11919
                     /locus_tag="VI319_15600"
     CDS             10966..11919
                     /locus_tag="VI319_15600"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY6865319.1"
                     /translation="MHDPHVPPRRFTGIRRKISLFMLTAVVGVAGAVALVAYKQGAFV
                     RHTAIYFYAADVFGINKGMAVRLFGLPVGSVRELQIADRGVKVELSIMTEYVSRVPKS
                     STARLTREGYIGAGTIQIVPGGDPTASSEPVHEGDVIAFVPNRGVAELVEDIKVQLTP
                     MVNEMRRMFAEMNAPGSDLRKSLQGASVVFQQLPETNREMRDVLRDADRAVRATQTTM
                     ASAARVTDPLEKDLPAVTATLGTALDSLAGAAHEIRTVARSNGDALHDTLRDGREVVG
                     AAKSSWLIRDYIESPAMRTLPVDSFESFGAGPEVKAVKAAR"
     gene            11921..12781
                     /locus_tag="VI319_15605"
     CDS             11921..12781
                     /locus_tag="VI319_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865320.1"
                     /translation="MHRGARTAALACMLLAACGSQPAKPKPPAVSTALEANRRAEAQL
                     RGGDLEGAARGYREALRASQALEDPDGIAANAINLSIVYQRLGRLADARTSLAPVLEQ
                     PNLKFAPERLAQASLRRALIDLDERRYASAGEWAGRAESQCGACPLSAAIHNVQGQLA
                     LESGRLDAAGAGAQRALDASRASGDRAETANALRLMGLVALRSGDGTAAAARMGEALA
                     VDRDLAAPRKIYLDLVGLGRASVLLGERDAARAYYERALAVSEAERDTRGIAEASALI
                     RTLEQKPASQ"
     gene            complement(12791..13375)
                     /locus_tag="VI319_15610"
     CDS             complement(12791..13375)
                     /locus_tag="VI319_15610"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6865321.1"
                     /translation="MNSARRGLLVAGAAWLAGCSLLRRGEPTVITGGESPTLIPEVPY
                     FPTPEPVVDAMLKLARVGPADLVYDLGCGDGRIVITAAAKYGARGFGVDIDPAPLIRA
                     RHDAERAGVDERVHFERGDLFLTDLRPATVVTLYLYESLNRRLMPKLRSELRPGARIV
                     SHKFGMGTQWPPDSVLSVGDSTIYLWTVRGPAAA"
     gene            complement(13372..>14510)
                     /locus_tag="VI319_15615"
     CDS             complement(13372..>14510)
                     /locus_tag="VI319_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY6865322.1"
                     /translation="ELATAYPSAGGEYHFIGRAFGRVPAFLYGWSRMTVIVVGPMAGF
                     AFVFGDYCARLFSLGGYSSAIYAAIVIVVLTWVNIVGIRAGKTTQNVSTALLVIGLIV
                     VIAVGLFSGAAPEAAAPAPAAASLSGIGLAMVFVLYTYGGWNDAAYISAEVRERERNM
                     SRSLFYSLGVVTVLYVLINLAYVKGLGFAGVAKSDAVAADLLVRFWGAPGEKLISVVV
                     ALATLTSINGTMIVGARSNYALGREWPFLRLLGHWNEATGSPRNAYLVQGAIALLLVG
                     IGTRERKGFETLVEFTAPVFWTFFLMTGLALFVLRARDAGTSRPYKVPLYPVTPALFV
                     AMCAYMLYSSLRYTGAGALVGVGFVAAGAVVLAVLGRRTAPSQA"
BASE COUNT         2150 a         5096 c         5084 g         2180 t
ORIGIN      
        1 cgcgctctgg gccggcgtgc cgctgatcac ctgccccggc gagcacccgg tttcgcggct
       61 gggtgcgagc ctgctccacg ccatcgggct cgacgagctg gcggcgcgga gcgtcgcgga
      121 gtacgagtcg ctggcgatcg atatcgccac acacccggac cggctgcgcg cacagaagca
      181 gaagctcgcg tcgaaccggg agacccatcc gctgttcgac atcgcacgtc tcgtgcgcaa
      241 cctcgagcgg gcgtacctgg aaatgcaggc gctgcaagag gcgggcaggc cgccgcgctc
      301 gttcgacatc gaggaccccg cgccgtttgc cgctcctgcg gaattccgct agcctgcggc
      361 catgagcgat cagaacaggg tcacgcttcc caagggcaag gatcccctgc tgcgcgtcgt
      421 ccccatgccg cacgacgcga accagcacgg cgacatcttc gggggctgga tcatggcgca
      481 ggtcgacatc gccggcggcg tgctggccgc gcgtcacgcg cgtgggcgcg tcgcgacggt
      541 ggcggtgaac cagttcgtgt tcaagcagcc ggtgttcatc ggcgacgtgg tgtccttcta
      601 cgccgacatc acgcgcgtcg gcaggacgtc gatcaccgtg aacgtggagg tctacgcaca
      661 gcgcaacccc gcggacgtcg agacggtcaa ggtgaccgag gcggttctca cctacgttgc
      721 aaccgaccac aaccgcaagc cgcgccccgt cggccccagc ggccgcgggt gatcagagct
      781 tgacgagctg cgcgaagatg tcctgcgcgc cgtcgacgta aaacaggcag gcgaaatccc
      841 gctgggtctg ctcggcgtcg cggaactgct cgtggacgga aaagcggtac tgcacatcga
      901 agtcgcaggt gtagaaatcg ttgtgcgggg cgtgctggct gcgccaggcc ctgttcagat
      961 aggggaacca gaagggggac acgggcggcc actggtgcgt gaggtcgcca taagctcgcg
     1021 cactggccca gttgggtgcc acgatcgtcg ccttggcacc ggttcgcagc acccggtaga
     1081 gctcgttcac gaaatggatg cgctcgggcg ccgtgaggtg ctcgacgaaa tgcgaagaga
     1141 acgcttcgtc gacgctgccg tcgtcccagg gccacggtcc gcgaaggtcg gcgacgacgt
     1201 cgacccctgg aaactcccga acgtcgactc cgacgaaccc cggctgcttg ctcgcgccgc
     1261 aaccgaggtc gagcttgagg gatccggcgc cggcgcccat cgccagccta gatcttcaac
     1321 agccgcttcg cgttccccga gcagatcttg acccggtcct cgaggctcag gggcaggtcg
     1381 gcgagccact tcgttcccgg cgggttgagc acgaacggat agtcgaccga gaacagcacg
     1441 cggtcgacgc ccatctccat gatcgagcac atcagcgccg gcgtggagaa gttgccgctg
     1501 gtcgtgatcc agaagtgcga gtcgaaggtg tcgcggaagc tgctcggctt gccgccgcgc
     1561 gacaggcaca tgtcgatgcg ccatgccgag aacggcagcg actccccgag atggcccagg
     1621 atgatcttgg tcccgggata cttgtcgcac actcccgaga gcacgaggcg cacacccatc
     1681 gtggcggtct cgacggtgta gccccaggcg gcgctgacga tctgcgggaa gtccttgatg
     1741 taatccttgt aataggcgtc gatcaccgcc tgcatcggcg tcgcgggatg gatgtagatc
     1801 gggacgtcga gctcctgcgc gcgctcgaag atcggccaga agcgcttgtc gtcgatgaac
     1861 acgccgttcg acggcccgtg gatcatggcg ccgacgaagc cgagcttgcc cacgcagcgc
     1921 tcgagctcgt cggccgccgc cttgggatcg gccgtgggca gcgcggcgaa cgcgcggaac
     1981 cggttcgggt gctcgcgcac ggcctcgcgc agccggtcgt tcgcgcgccg ggcgagcggg
     2041 atcgcggtct ccgcgtcgag gcgctgcgtc gacggcgcgc cgtgcgagag gacctgcacg
     2101 tcgatgccgg cctcgtccat ctctctcagg cgcagctcgc cgaggtcccc gagccgccgg
     2161 cgcaggtccg ggttgcggcc ttcggcgcct tcgaagtgct tcgccacctc ggcgtcgtag
     2221 tagtgctcct cgagcgcgat cacgaacggt cttgagttgc tggccacggt ttcctcatct
     2281 cccggtaaaa gaaacggggc gcccggggcg cccctgcatc ggatgctgcg ctccggattc
     2341 tatcgcgcca ggatctgcca ggcgaagatc atctgggcga tccacccggc cgcgaacgcg
     2401 agcgtgcgca cccagggcac ggcgaacgtg taggcgagca cgtgcaccag gcgcgcccag
     2461 aagtagaggg cggcggcggt cgccgtcgcg ccgttcgaca cgcccagctg ctgcgccgcg
     2521 agcacgaggg ccgcgaacac gacgaggttc tcgacagcgt tggcgtgcgc cttcatcatg
     2581 cgcacggccc acggcgactg cggcttcggg ctcgccggat agccgaccgc gtccatcagc
     2641 ccccagaccg cgatgcgatc gaggatgtac ggaacccaca tcaacgcggt cactacggtc
     2701 acccacacca agtacatcag ctccgctttc atgtttcccc ctgtttggtt aggacgcgga
     2761 ctgcttcttc tccccgggta ctgcgatgtg cttcgaaagc tccagcttgg cgatcgcgtt
     2821 gcggtgtacc tcgtcggggc cgtcggcgag gcgcagcgtg cggttccagg cgtacatgcg
     2881 cgccagcgga aagtcgtccg ccattcccgc cccgccgtgc gcctggatcg cccagtcgat
     2941 gacgcgctcg gcgacgttgg gcgcgagcac cttgatcatc gcgatctcgg cgcgcgcggc
     3001 cttgttgccg accgtgtcca tcatgtcggc ggctttcagg accaggagcc gcgccgaatc
     3061 gatcatgatg cgggcctcgg cgatgcgctc gcgggtcacg ccctgctccg cgatcggctt
     3121 tccgaacgcc acgcgtccga gcgtgcgccg gcacatcagc tcgagcgcgc gctcggccgt
     3181 gccgatgatg cgcatgcagt ggtgtatgcg cccggggccg aggcggccct gcgcgatctc
     3241 gaagccgcgg ccctcgccga ggagcaggct ggccgcgggg acgcgcacgt cgcgcagctc
     3301 cacctccatg tgtccgtgcg gcgcgtcgtc gtagccgaag atggtgagcg gcctcaggat
     3361 cttcacgccg ggcgcgtcgc gcggcaccag gatcatcgac tgctgcgcgt ggcgcacggc
     3421 gcccggatcg gtcttgccca tgacgatgaa caatttgcag cgcggatcgc cggcgcccga
     3481 ggaccaccat ttgcggccgt tgattacgta gtggtcgccc tgccgctcga tgcggcagcg
     3541 gatgttggtc gcgtcgctcg aggcgacgtc gggctcggtc atgaggaagg cggagcggat
     3601 cctgccctcg agcagcgggt cgagccactc gcgcttctgc gcctcgctgc cgtagcgctc
     3661 gagcgtctcc atgttgcccg tgtccggtgc cgagcagttg aacacctcgg gcgcccagtg
     3721 cacgcggccc atgatctcgc acagcggcgc gtactcgagg ttcgagagcc cctgcggcgc
     3781 gcgcggcgag cgcggcagga aaagattcca caggccctcg gcgcgcgcct tcggcttgag
     3841 ctcctcgatc agccgcgtcg gcacccaggc gtcgcccttc gcgcggttcg ccgcgacttc
     3901 ctcgtggaag cggcgctcgc tcggatacac gtgcgcgtcc atgaaacgcc ggagcttgtc
     3961 ctccagcgcg cgcaccttcc cggagtacgc gaaatccatc tgtctcccgt ttcaggcttt
     4021 ctgcgcgtat tcccagccga gctcggcgag cgggcgcgcc cgccggccgg catcgagcgc
     4081 ctgctcgctc gccgccgtgc cgtcgagcgc ccgcttcatg atcccctgca ggatggcggc
     4141 gatcctgaac aggttgtacg cgaggtagaa gtcccaatgc tcgacggccg cgcgccccgt
     4201 ccgcgcgcag tatgccgcaa cgtaggcttc ctcgcgcggg atgccgagcg cgtccaggtc
     4261 gagcccgccg atgccgcgga agcgccccgg cggtatgtgc cagctcatgc agtgataggc
     4321 gaagtcggcg agcgggtggc cgagcgtcga gagctcccag tcgagcacgg ccaggatgcg
     4381 cggctcggtc gggtggaaga tcaggttgtc catgcggtag tcgccgtgca cgaccgcggt
     4441 ctcctcgccg ggcgggacgt gcgcgggcag ccagtcgatg aggcggtcca tcgcctcgat
     4501 cttctccgtc tcggaggcct ggtactgctt cgtccagcgc ccgatctgcc gcgcgaaata
     4561 gttgcccggc ttgccgtagt cggcgaggcc gaccgcggcg acgtcgacgc ggtgcagcgc
     4621 cgcgatcacg cgattcatct cgtcgtaggt cgcgccacgc tcggcgcgcg tcatcccggg
     4681 cagagactgc tcccacagca cgcgtccctc gacgcactcc atgacgaaga aggcgcgccc
     4741 gatcacgctc tcgtcctcgc acagcgcgag cgtgcgcggc accgggatcc cggcgcgcgc
     4801 gagcgcgctc atgacgcggt actcgcgctc gaccgcgtgc gccgatggca gcagcttcgc
     4861 ggcgggaccg ggcttggcgc gcacgacgta ggcgcgtccg cccgcggtca ggcggaacgt
     4921 ggggttggac tggccgccgc ggtactgctc ggccgacagc ggcccggaga acccttcgac
     4981 gtgcaggcgc agccagcgct cgagcgcggc cggatcgaag cgatgacgct cctgcaccgg
     5041 catggtgccg gtgaacgggt cgcgggccgg ggatggctgt tcgcgggggg cgctcactgc
     5101 tcgcgccatg ctagcaccgc tgcccgggtg tcgtcccggc gacgctcgcg gcagggcccg
     5161 ggaacgcccg cccaagctgt tatccgcgcc gggacaagga cttggaaaac gcggcggctc
     5221 tggcccgtct cttgctcatc ccggggtagg aggatacgaa catggtcgaa acgatcgtct
     5281 ttctgatgca gatgatgtcc gcgctcgtaa tcgtcgccgg cggcgtcctg accttctcgc
     5341 acgtgctcga gcaggaaggc gtggcagcgc cgagcctcga ccgcgccaac gatttcgaga
     5401 tcgacttccg gcgcgtgctg tccttcgcgc gcctggcagc gcagcgctaa gccgtcccga
     5461 agcacctgcg ttcgccgccc cggccggggc ggcggcgtcg ttcgggtccg cggtatcatt
     5521 cgggccgcca tgccgccgtc ccggacgctc accttcttcc tcaacgccgg gcacgcgttc
     5581 gaccacctgc tgatgctgat cttcccgacc gtggtgctcg ccatgggttc ggagctcggc
     5641 ctcggctacg cggagctgct gccgctctcg ctcggcgggt tcgtcgcctt cggtgcgtgc
     5701 tcgattcccg ccggctggct cgcggaccgc tggagtcgct acggcatgat gctcgtgttc
     5761 ttcttcggca tcggcgcggc gagcctcgtc acggggttgg cgcgcacgcc ggcgcagatc
     5821 gcggtcggcc tgactctggt cggcgtgttc ggcgcgatct accacccggt cgggatcgcc
     5881 atgctggtcg cgagccagcc gcgcgtcggg cgcgtgctcg gcgtcaacgg agtctggggc
     5941 aacgcgggcg tcgccttctc ggcgctcatc gcgggcgcgc tcgccgacac gctcggctgg
     6001 cgcgcggcct tcttcgttcc cggcgcggcg gccatcgccg cgggcgccgc gttcgcgttc
     6061 ctcgtgcgcg acccgggccg cgggacgccc ggacagaaga cggcgccggt ccgcctctcg
     6121 cgcgaggacc tggtgcgcgt gttcgccgtt ctcactgtcg ccacggcgtg cggcggagtc
     6181 atcttccacg cgaccaccgt cgccatgccg aaggtcttcg aggagcgcct tggcgcgctc
     6241 acgcacacga cgttcggcat cggctccctc gtgtgcggcg tctatctcgt cgccgcgctc
     6301 gcccagctgt gcgtcggctg gtggctcgac cggcgtccgc tcaagtcggc gttcatcccg
     6361 gtggtggcgc tgcaggtgcc gctgctcgcg ctcgcgggca ccgcgcacga cctcgccatg
     6421 gtcgccgtcg cggtcgcgat gatgttcttc gtgttcggac agatcccgat caacgacgcg
     6481 atgatcgcgc gctatacggc cgaggaatgg cgcgcccggg cgtacgcggt gcgctacgtc
     6541 gtgtccttcg gcggcagcgc gctcgcggtc ccgctgatcg cgtggctgta ccgcacgagc
     6601 ggcgacttcc gcacgctgta tttcatgctg gccgcgctgg cgctcgcggt gctcgtcgcg
     6661 gcgctgatct tcccgggcga gcccaagccg gctcgcgccg ggcaggcggc gtagtccgaa
     6721 cgcgaattcc ggacaaaaaa aagccgccgc ggttcgcggc ggctcttcgt cgcgcgggaa
     6781 ggctcagggc ttgagctgcg cgcggatctc gccgcccttg tgggccgggc tgtgcacgtt
     6841 gacgtacagc tcgcccgcct tgtaggcgtc gtactgggca tcggtcagct tcgcgcccgc
     6901 gggcaccagc cattcgtcgc ccttcttcgt gagcggaacg atcaccgggc cgttgcggcc
     6961 gctcgcgccc tggtggatgt gcgcggcgac accgttgatc ccgctggtcg tcaccgagcc
     7021 gctcacgctc ttgtcggccg cgaccttgat gttcgcggtg cccttggcgg cggtcgtcac
     7081 cgggggcact tcctgcgcgc cggtcaggtc gaccttgccg ccgcccatcc agcccttgat
     7141 ggtctggcag cccgagagcg cgagcgcggt tgccgcaatg gaagcaagta cgaagctgcg
     7201 gaattgcatc tgcatggtgg ttcctcctct agcgtccgta aacaggggcg cgcagagtat
     7261 attcccaaag cgccctccgg gcggtgaggc ttaccacact gccccccgtg cggcgatcgg
     7321 ccataccgat tccaccttcc cccggcgcac gcaaaccagc cagtcgtgca ggtttaccgt
     7381 cggatcgcaa tgccccggca cgagcagcag ccgctcgccc agcgcgggca cgcgcgcatc
     7441 cggggaaacc ctgatcaccc cgtgctcgtc ggaggccctg acgtactcga gccccggcca
     7501 ggcgtggacc tcaggcagcc ccgagtcgac gctcgcggcc ttcaggcccg cgtcgacgac
     7561 gacgcgatcg gacgcgggcg cgctcatgac cgaagccagg aggaacaggc tctgatcgaa
     7621 gcgcggccag tcgtcgtccc agcggttgcg gctgtagtcg gcgtccatga agacgtacga
     7681 gcccggctgc acctcgttgt acgcgccgct cgcggcctcg agcaggaaag tgcccgtgcc
     7741 cgcgccggtc acggtctcgc agcgcagccc gatgccgtcg agcgcgcgcc gcgccgcgag
     7801 cgcgtgcgac gccgccgcga cgatcgcggc gcgccgctcc tcggggccgc gcaggtgctg
     7861 cgccgagccg tgatacgcgt gcaggccggc gaagcgcagg tgcctcgccc cggcgatgac
     7921 gcgcccgagc gcgagcacgc tctcgcccgg cgccacgccg cagcgcccgg cgccgacgtc
     7981 gagctcgatc agcacgtcga ggcggacgcc gcggcgcgcg gccaccgcgt cgagctgcgc
     8041 gacgttgcgc gcgtcgtcgg cgagcaccgc gacgcgcgcg acgtgcgcga ggcccgcgag
     8101 gcgctcgatc ttcaccgcgc cgacgatctc gttcgtcacc agcacgtcgc gcacgccgcc
     8161 cgcgatcatc gcttcggcct cgctcacctt ctggcagcac acgcccaccg cgcccgcggc
     8221 gatctggcgc ttcgcgatct cggggcactt gtggctcttc gcgtgcggac gcaggcgcac
     8281 gccgcgcgcc ttcatggcgt cggccatgcg cgcgacgttg cgctcgaacg cgtcgaggtc
     8341 gagcacgagc gcgggcgtat cgatgtcggt gagcgaggtg ccggcttcgg cgggcggcgg
     8401 gaacggcatg gcggcgaagc ccattgtaga atgaattttc gcagcgaccc tcccccgacg
     8461 attctcggac accctcgatg agccttcccg cgacgatgcg cgtggtcgag atcacgcagc
     8521 ccggaaaacc cgaggtcctg aagcccgccg cgcgagcgct gccgcagccg aagaccggcg
     8581 aggtgctggt caaggtcgag gcggcaggcg tcaaccggcc cgacgtgctc cagcgcaccg
     8641 gccactaccc ggtgccgccg ggcgcctccg acatcccggg cctggagatc gcggggcgcg
     8701 tcgccgcgac cggcgcgggc gtgagcgggt ggcgcgtcgg cgacgccgtg tgcgcgctcg
     8761 tccagggcgg cggctacgcg gagtactgca cggcgcccgc gctgcagtgc ctgccggtcc
     8821 cgaagggcct gtcggcgatc gaggccgcgt cgctgcccga gaccttcttc accgtgtgga
     8881 gcaatgtctt cgaccgcgcg cgcctcgcgc cgggcgagtc gttcctggtg cagggcggat
     8941 cgagcggcat cggcgtcacc gcgatccaga tggtgaaggc gttcggccac acggtgttcg
     9001 cgaccgcggg cagcagggag aagtgcgacg cctgcgaaaa gctcggcgcc gaccgcgcga
     9061 tcgactacaa gaccgaggac ttcgccgcgg tcgtgaagga ggcgaccggg ggccgcggcg
     9121 tcgacgtcgt cctcgacatg gtcgggggtg actacgtcga ccgcgagctg aaatgcctcg
     9181 cggacgacgg ccgcatcgtg ctgatcgcct ttctcggcgg caaccgcgcg aacctgaacc
     9241 tgaacgagat cctgcgccgg cggctggtcg tcaccggctc gacgctgcgc ccgcgcccgg
     9301 tcgagttcaa gggagcgatc gcggcgaagc tgcgcgagaa ggtctggccg ctggtcgagg
     9361 cgggccgcat caagccggtg atccacgaga ccttcccgct cgaccaggcg gcgcgggcgc
     9421 acgagctgat ggaatcgggc cggcacatcg gcaagatcgt gctcgtggtg tgaccgaaat
     9481 cccgcggcgg cgcgcggccc cggtgtaagc tagcgccggg aggaggagcc atgttcgccg
     9541 tctaccgcga gctcgaggga tccacgcggc tcgccgcgca cgtgctgcgg cggttctggg
     9601 tgttgcgctt cccgccggtg atggaggagc tggtgcgcca gatctacttc accggcgcga
     9661 gcgccaccgc gaccatcgtc gtgcgcggcg ccttcatcgg cacgctcatc atcgcctacg
     9721 tgatcgaggt gctcgaggcc gacgcggcgc tcgccgtgaa gatgctgctc tgggtggtgc
     9781 tgcgcgaggt cgggccgctc atcgccgcgg tgctcgtgat ccagcgcagc gggaccgcgg
     9841 tcgccaccga gctcgccctg atgaagatct cgggcgaggt cgacagcctg cggcacctgc
     9901 gcatcgatcc gcaggaccac ctcgtcgtcc cgcgcgtgct cggcatcgcg ctgtcgctcg
     9961 ccgcgctgac gctgtacgtg cagttcatcg cggtcggcgg cgggatggtt ctctcggcgc
    10021 tggtgatcga cgcctccttc agcgagcttg cgagcaactt cttcctgctc gcgagcccgc
    10081 tcgacctcgc gtactcgatc gtgaagagcc tgctgttcgg cggtgcgatc ggcgtgatcg
    10141 cgtgttacta cgggctgaac ccgcccggcc gctcgatcaa cgcggtcccg aaggcggcga
    10201 tcaacgcggt gtcgcagtcg acgatcgcgg tgatgatcat caacgtggtt ttcgcctacc
    10261 tcgcgtacgg cgtggccctc ttcggcctgt tcaccgcgaa gctatgagcc ccaggccatg
    10321 agcgagctgc cgactcgcgt cgaggaagcg cgccagatgg cgctcggcta cgaggcgccg
    10381 ccggagggcc cgtccggacg cctgtggata ccgtccggca cgggccaccg cgccgccccg
    10441 gtcagccagg cgttcaagca gcggctcgtc gacgcgctgc tcgatctcga gccgcagccg
    10501 ggggcgcggc tggccgtgct tggcgccgac gcgggcggcc tcgccgcccg cgagcgccag
    10561 gcgctgcgcg cgcgcatcgc cttcctgccc gcgaacggcg gcatgatcag taacctgaac
    10621 ggctgggaga acatcgtcct gccgctcggc taccgcgacg cgaagcgcgt gaacgccgcg
    10681 tttcccaagg tgcacgccct gatcgagcgc ctgggaggtc atccgcgcgc gctgctcgcg
    10741 aagctgcccg aggagatgac cctgtacgag aaaaaactcg cgggctatgt caggatactg
    10801 ctcgaggcgc cggagctggt ggtcgcagaa aacctcgcag gcggcctcga gccggaagat
    10861 cgcaggcgca ccgcggcgtt ccccgccgcc tatcatgccg agcgtccgga cggcgtgttc
    10921 gtgcagctcg acgacgcgcc cgacgactag ccagaggagg tcccgatgca cgatccgcac
    10981 gttccgcccc ggcgcttcac cgggatccga aggaagatca gcctgttcat gctgaccgcc
    11041 gtggtcggcg tcgccggcgc ggtggcgctg gtcgcgtaca agcagggcgc gttcgtgcgc
    11101 cacacggcga tctacttcta cgccgccgac gtcttcggca tcaacaaggg catggcggtg
    11161 cggctgttcg gcctgccggt cggcagcgtg cgcgagctgc agatcgccga ccgcggcgtc
    11221 aaggtcgagc tctcgatcat gaccgagtac gtgtcgcgcg tgccgaagag ctcgaccgcg
    11281 cggctcacgc gcgagggcta catcggcgcg ggcaccatcc agatcgtgcc cggcggcgac
    11341 ccgacggcgt cgagcgagcc ggttcatgaa ggcgacgtca tcgccttcgt gccgaaccgc
    11401 ggcgtcgccg agctggtcga ggacatcaag gtgcagctca cgccgatggt gaacgagatg
    11461 cgccgcatgt tcgcggagat gaacgcgccg ggaagcgacc tgcgcaagtc gctgcaggga
    11521 gccagcgtgg tgttccagca gctgcccgag accaaccgcg agatgcgcga cgtgctgcgc
    11581 gatgccgatc gcgccgtgcg cgcgacccag acgacgatgg cctccgccgc tcgcgtcacg
    11641 gacccgctcg agaaggacct gcccgcggtc accgccacgc tcgggaccgc gctcgactcg
    11701 ctcgccggag cggcccacga gatccgaacc gtggcccgca gcaacggcga cgcgctgcac
    11761 gatacgctgc gcgacggccg ggaggtggtg ggcgcggcga agagcagctg gctgatccgc
    11821 gactacatcg agtcgccggc gatgcgcacg ctgccggtcg acagcttcga gtcgttcggc
    11881 gcaggacccg aggtcaaggc cgtgaaggcc gcgcgctgac atgcatcgcg gcgctcgcac
    11941 ggcggcgctc gcgtgcatgc tgctcgccgc ctgcggatcg cagccggcga agcccaagcc
    12001 ccccgcggtc tcgacggccc tggaagccaa ccgccgcgcc gaggcgcagc tgcgcggcgg
    12061 cgacctggaa ggcgccgcgc gcggctaccg ggaagcgctg cgcgcgtcgc aggccctcga
    12121 ggatcccgac ggcatcgcgg ccaacgcgat caatctttcg atcgtctacc agcgcctcgg
    12181 caggctcgcc gatgccagga ccagcctcgc gcccgtgctc gagcagccga acctcaagtt
    12241 cgcgcccgag cgcctcgcgc aggcgtcgct gcgccgcgcg ctgatcgacc tggacgagcg
    12301 ccgctacgcg agcgccgggg agtgggccgg acgcgccgag tcgcagtgcg gcgcgtgccc
    12361 gctgtcggcc gcgatacaca acgtgcaggg ccagctcgcg ctcgagtccg ggcgcctcga
    12421 cgccgcgggc gcgggcgcgc agcgggcgct ggacgcgagc cgcgcctcgg gggaccgcgc
    12481 cgagaccgcc aacgcgctgc gcctgatggg gctggtggcg ctgcgctccg gcgacgggac
    12541 ggccgccgcc gcgcgcatgg gcgaggcgct cgccgtcgat cgcgacctcg cggcgccgcg
    12601 caagatctac ctggacctcg tcggcctggg gcgggcgagc gttctgctcg gcgagcgcga
    12661 cgcggcgcga gcgtattacg agcgggcgct cgcggtgagc gaggccgagc gcgatacccg
    12721 cggcatcgcc gaggcgagtg cgctcatcag gacgctggag cagaaacccg cgtcgcaata
    12781 gcggccgtct tcaggcggcc gcgggccccc ggaccgtcca gaggtagatc gtgctgtcgc
    12841 cgacgctcag cacgctgtcg ggcggccact gcgtccccat gccgaacttg tgcgagacga
    12901 tgcgcgcgcc cgggcgcagc tccgagcgca gcttgggcat gagccgccgg ttcagcgact
    12961 cgtaaagata cagcgtgacg acggtggccg gacggaggtc ggtgaggaac aggtcgccgc
    13021 gctcgaagtg gacccgctcg tcgacgccgg cgcgctcggc gtcgtgccgc gcgcggatga
    13081 gcggcgccgg gtcgatgtcg acgccgaagc cgcgcgcgcc gtacttcgcc gcggcggtga
    13141 tgacgatgcg cccgtcgccg cagccgaggt cgtagaccag atcggcggga cccacgcgcg
    13201 cgagcttcag catcgcgtcg accaccggct cgggcgtggg gaagtacggc acctcgggga
    13261 tgagcgtggg cgattcgccg cccgtgatca ccgtcggctc gccgcggcgc agcagcgagc
    13321 agccggcgag ccaggccgcg cccgcgacga ggagaccgcg acgtgcggaa ttcatgcttg
    13381 ggacggcgcg gtcctgcggc cgagcacggc gaggaccacg gcgccggcgg cgacaaaacc
    13441 cacgccgacc agtgccccgg cgcccgtata tctgaggctc gagtagagca tataggcgca
    13501 cattgcgacg aacagcgcag gagtgaccgg gtagagcggc accttgtacg gtcgcgaggt
    13561 gcccgcgtct ctcgcgcgaa gcacgaacaa ggctagaccc gtcatcagga aaaacgtcca
    13621 gaacaccggc gcggtgaact cgacgagcgt ctcgaacccc ttgcgttccc gagtgccgat
    13681 gccgaccagg agcagcgcga tcgctccctg gaccaggtag gcattgcgcg gcgacccggt
    13741 cgcctcgttc cagtgcccga gcagacgcag gaacggccac tcgcggccga gagcatagtt
    13801 cgagcgcgcg ccgacgatca tcgtgccgtt gatcgacgtg agcgtagcca gcgccacgac
    13861 cacgctgatc agcttctcgc cgggcgcgcc ccagaatcgc accaggaggt cggccgccac
    13921 cgcgtccgat ttcgccaccc ccgcgaaccc caggcccttg acgtacgcga gattgatgag
    13981 cacatagaga acggtaacga cgccgagcga gtagaacagc gagcgcgaca tgttccgctc
    14041 ccgctcgcgc acctccgccg agatgtatgc agcatcgttc caaccgccgt aggtgtacag
    14101 cacgaacacc atcgcgaggc cgatgccgga cagcgacgca gcggccggcg cgggtgcggc
    14161 tgcctcgggc gccgcgcccg agaacaagcc caccgcgatg acgacgatca gcccgattac
    14221 cagcagtgcg gtggacacgt tttgcgtcgt cttgccggcg cggatgccga cgatattcac
    14281 ccaggtcagg acaacgatga cgattgccgc atagatggcc gaggagtacc cgccgagact
    14341 gaacaggcgc gcacaatagt cgccgaagac gaacgcgaat cccgccatcg gtcccacgac
    14401 gatcaccgtc atgcgcgacc agccgtagag aaaggccggc acgcggccga aagcgcggcc
    14461 gatgaaatgg tactccccgc ccgcgctcgg atatgcggtc gcgagctcgg
//
LOCUS       DATDMW010000373         7629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2477690, whole genome shotgun sequence.
ACCESSION   DATDMW010000373 DATDMW010000000
VERSION     DATDMW010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477690"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VI319_15620"
     CDS             complement(<1..1017)
                     /locus_tag="VI319_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY6865323.1"
                     /translation="MNEISRTGLSAAEARARLAADGPNAVPARDRRDTLAIVLSVVRE
                     PMFVLLIAGAVLYLLLGDVREALVLGASIVVVTVITVAQERKAERAVEALRDLSSPRA
                     VVVRDGREERIAGPEVVRGDLAVLAEGDRVPADGRLLAANDLMIDESLLTGESVPVGK
                     SAGGTDDERDAHVYSGTLVVKGHGRAVVTATGARAELGRIGASLAALEPEKTALERET
                     GRIVRFIAGVAVALSVLLAAFYVAKGGDALSGVLSGITLAMAILPEEFPVVLTVFLAL
                     GAWRIARRGVLTRRMPAIEMLGAATVLCVDKTGTLTENRMTVVEVYDRSQWHAPGPGG
                     ATAQV"
     gene            1141..1431
                     /locus_tag="VI319_15625"
     CDS             1141..1431
                     /locus_tag="VI319_15625"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEY6865324.1"
                     /translation="MASKQKSDVNPGGPATDRPPRKVREAARPGAKPEAPRAEGDGIA
                     RLSEDELNAMISRAAYLRAERRGFEPGHEVEDWLAAEAEIRATMSRGSQQNR"
     gene            complement(1437..3809)
                     /locus_tag="VI319_15630"
     CDS             complement(1437..3809)
                     /locus_tag="VI319_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY6865325.1"
                     /translation="MEHAAHENTHHSHAHGDGATPPPGKAVDPVCGMTVDIATAKHSH
                     VHGGKTWYFCNPRCREKFVADPGRYAAPAHARADPPAPAGTLYTCPMHPEVERQGPGN
                     CPICGMALVPMAGASEDDTELRHMTRRFWVSAALTAPLAVIAMAPYFGIRQPFGILEH
                     ARMYLELALGTPVVLWGGWPFFVKFWRSLAHRSPNMYTLIGLGVALAYLFSVAAVFAP
                     GLFPQEFQEESGQVGAYFEAAAVIVTLVLLGEVMQLRAIGQTSRAIRELLALAPNTAV
                     RIEADGREREVPLAEVKVGDRLRVRPGDKIPVDGTCVDGASNIDESMITGEPVPVAKK
                     PGDRVTGATINGRGTLVIRAERVGSDTLLSQIVHMVARAQRTRAPVQRLADLVAAYFV
                     QTVIAIAALTALVWWFFGPEPRLAYAVVNAVAVLIIACPCAVGLATPISITVAMGQGA
                     VNGILFRNAEAVEKLREIDTLVVDKTGTLTLGSPRLVDLVVEPVIAEPEALALIASLE
                     RASEHPLAQAIVRGAEERGLKLSAVDKFESVTGQGVRGEIGGRKVEIGSRRFMEGRGS
                     LPASLAEKADALRTQGKTALYAAIDGRPVAVIAVADPIKETTPEAIDALRAEGVRVVM
                     LSGDSRKTAEAVGRELGIDQVIAEVLPEQKVMHIEALQGGKDGAPRRAVAMAGDGIND
                     APALARADVGIAMGTGTDVAIETGSVTLVKGDLRGIVKAIRLSRATMRNVRQNLFFAF
                     VYNALGVPIAAGALYPVFGLLLSPIFAGAAMAMSSVSVVTNALRLRRVRL"
     gene            complement(3820..4383)
                     /locus_tag="VI319_15635"
     CDS             complement(3820..4383)
                     /locus_tag="VI319_15635"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY6865326.1"
                     /translation="MSHVATPAGHRFPWYVRLILRLQRRRYGRELEPSRLWGRLPRAF
                     LAMSLMYGVLDRKSSPIEPALRSLVQVRVSQINGCAFCVDINAASSLGRGADGRKLAE
                     LARYADSGAFSEREKAALRYAEAVTDSRTRTDAAMIAALKSHFDDDAIIELTALIAYQ
                     NMSSKFNAALDVPAQGFCALAGVRQPH"
     gene            complement(4483..4848)
                     /locus_tag="VI319_15640"
     CDS             complement(4483..4848)
                     /locus_tag="VI319_15640"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEY6865327.1"
                     /translation="MKRLAVLAPVLFLAAAHPSWAQQGGGMGGMDMNDRQGAKPAPKT
                     AAAQGKTHKGSGTVTKVDPAAGKVTIAHGAIGTLKWPAMTMTFSVSDKALLDHVHPGG
                     KVDFEFVQKGQDYVINKIH"
     gene            complement(4845..>7629)
                     /locus_tag="VI319_15645"
     CDS             complement(4845..>7629)
                     /locus_tag="VI319_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY6865328.1"
                     /translation="TGVGWVYEYALVDRSGRMDLSQLRALQDWFLKYELKAVPNVAEV
                     ASVGGMVRQYQIVLDPARLRAFNIPQAKVVEAVQRANQETGGSVLELGEAEYMVRASG
                     YLRSLDDFRKIPLVTTEAGVPVRLGDVAQVQVGPEMRRGIAELNGEGEVAGGIVLMRS
                     GKNALETIAGVKAKIESLRPSLPPGVEIVPTYDRSSLIKRAVGNLGQKLLEEFVVVAL
                     VCALFLFHLRSAFVAIVSLPLGILASFIVMHTQGVNANIMSLGGIAIAIGAMVDAAVV
                     MIENAHKKLEAWAHAHPGERLDGEARWAVIGEAAAQVGPALFVSLLVITFSFVPVLTL
                     EAQEGRLFAPLAFTKTYAMAAAAGLAVTLIPVLMGYLIRGRIPEEQRNPISRSLIALY
                     RPALAGALARPKTALAIALVLLAATLWPMARMGGEFMPPLDEGDLLYMPTALPGLSVG
                     KASELLQQTDRLIRTVPEVASVFGKAGRAETATDPAPLEMFETTIQLRPRDQWRPGMT
                     MERLVEELDRTVQVPGLSNIWVPPIRNRIDMLATGIKSPVGVKVAGASLAEIDRLARE
                     VERAVRQVPGVSSALAERLGGGRYIDVDIDRDAAARYGLNVADVHGVVSAAIGGDNIG
                     ETVEGLQRFPISVRYPREIRDSVEKLRSLPMLTERGAQIRLGDVAAVRIADGPPMLRS
                     ENARLSGWVYVDIRGRDLSSAVREMKRTVAEQVQLPPGYSISWSGQFEYLERAAARLE
                     VVVPATLAIIFVLLYLAFRSGADALLIMATLPFSLVGGFWLMYLLGYNMSVASAVGFI
                     ALAGVAAEFGVVMLVYVKSAVEQRLAGGKPLGDAELKSAIDEGAALRVRPKAMTVAVI
                     LAGLLPIMASDGAGVDVMQRIAAPMIGGMVTAPLISLFAVPVAYYLMRRPRARARAVR
                     APRPQSQLKGETK"
BASE COUNT         1136 a         2765 c         2664 g         1064 t
ORIGIN      
        1 cacctgcgca gtcgcaccgc ccgggccggg cgcgtgccat tgcgagcggt cgtagacctc
       61 gacgaccgtc atgcggttct cggtcagcgt cccggtcttg tccacgcaca gcacggtggc
      121 ggcgccgagc atctcgatcg ccggcatgcg gcgcgtgagc acgccgcggc gcgcgatgcg
      181 ccaggcgccg agcgcgagga acacggtgag caccacgggg aactcctcgg gcaggatcgc
      241 catcgcgagc gtgatgccgg acaggacgcc ggagagcgcg tcgccgccct tggccacgta
      301 gaacgcggcc agcaggacgc tcagcgcgac cgcgaccccg gcgatgaagc gcacgatgcg
      361 cccggtctcg cgctcgagcg cggtcttctc cggctcgagc gcggcgagcg aggcgccgat
      421 gcggccgagc tccgcgcgcg cgccggtggc ggtgacgacg gcacgcccgt gtcccttgac
      481 gacaagggtg cccgagtaga cgtgcgcatc gcgctcgtcg tccgtgccgc ccgccgattt
      541 cccgaccggg accgattcgc ccgtgagcag cgattcgtcg atcatgaggt cgttcgccgc
      601 gagcagccgg ccgtcggcgg gcacgcggtc gccctcggcc agcaccgcga ggtcgccgcg
      661 cacgacttcg gggccggcga tgcgctcctc gcggccgtcg cgcacgacca ccgcgcgcgg
      721 gctggagaga tcgcgcagcg cctcgaccgc gcgctcggcc ttgcgctcct gcgccacggt
      781 gatgacggtg acgaccacga tcgaggcgcc gagcacgagc gcctcgcgca cgtcgccgag
      841 cagcaggtac agcacggcgc ccgcgatgag cagcacgaac atcggctcgc gcaccacgct
      901 cagcacgatg gcgagcgtgt cgcgccggtc tcgcgccggg acggcgttcg gcccgtcggc
      961 ggcgaggcgc gcccgcgcct cggcggcgct gaggccggtt cgggagatct cgttcacgcc
     1021 gacgaccttc ggacgcgcgc ggccctcgtg ccttgatgca cgtcaaaccg cccggcgatc
     1081 cggtggaacc agtcccccgg ccccggctct aaccacggta tccgcttcaa ggggaacacc
     1141 atggcgtcga agcagaaatc cgatgtcaac ccgggcgggc ccgcgaccga tcgtccgccc
     1201 cgcaaggtcc gagaggcagc caggccgggc gcgaagcccg aggcgccacg cgccgagggc
     1261 gacggcatcg cccggctgtc cgaggacgag ctgaacgcca tgatctcacg cgcggcctac
     1321 ctgcgcgccg agcggcgcgg cttcgagccc ggccacgagg tcgaggactg gctcgcggcc
     1381 gaggccgaga tccgggccac gatgtctcgg ggctcgcaac agaatcgctg agccgctcac
     1441 agccggacgc gccgcagcct gagcgcgttc gtcactaccg acaccgagct catcgccatc
     1501 gccgcgccgg cgaagatggg cgacagcagc agcccgaaga cggggtagag cgcgccggcc
     1561 gcgatcggca ccccgagcgc gttgtagacg aacgcaaaga agagattctg ccgcacgttg
     1621 cgcatggtcg cccgcgagag acggatcgcc ttgacgatgc cgcgcaggtc gcccttcacg
     1681 agcgtcacgc tgccggtctc gatggcgacg tcggtaccgg tgcccatcgc gatgcccacg
     1741 tccgcgcgcg cgagcgcggg cgcatcgttg atgccgtcgc ccgccatcgc gacggcgcgg
     1801 cgcggcgcgc cgtccttgcc gccctgcagc gcctcgatgt gcatgacctt ctgctcggga
     1861 aggacctcgg cgatcacctg gtcgatgccg agctcacggc cgaccgcctc ggccgtcttg
     1921 cgcgagtcgc ccgagagcat gaccacgcgc acgccctcgg cgcggagcgc gtcgatcgct
     1981 tccggggtgg tctccttgat cgggtcggcg acggcgatga cggcgaccgg gcggccgtcg
     2041 atcgccgcgt agagcgcggt cttgccctgc gtgcgcagag cgtcggcctt ctcggcgagc
     2101 gaagcgggca ggctgccgcg tccctccatg aagcggcggc tcccgatctc gaccttgcgg
     2161 ccgccgatct ccccgcgcac gccctggccg gtgaccgact cgaacttgtc gaccgcggag
     2221 agcttcagcc cgcgctcctc cgcgccccgc acgatcgcct gcgcgagcgg gtgctcgctg
     2281 gcgcgctcga ggctcgcgat cagcgcgagc gcctcgggct cggcgatcac gggctccacg
     2341 accaggtcga cgaggcgcgg gctgcccaat gtgagcgtgc cggtcttgtc gacgaccagc
     2401 gtgtcgatct cgcgcagctt ctcgaccgcc tcggcgttgc ggaacaggat gccgttgacc
     2461 gcgccctgcc ccatcgccac ggtgatcgag atcggggtcg cgagcccgac cgcgcaggga
     2521 caggcgatga tcagcaccgc gacggcgttg accaccgcgt aggcgagccg cggctcgggc
     2581 ccgaagaacc accacacgag cgcggtgagc gcggcgatcg cgatgaccgt ctgcacgaag
     2641 tacgcggcga cgaggtcggc caggcgctgc accggggcgc gcgtgcgctg cgcgcgggcg
     2701 accatgtgca cgatctgcga gagcagcgtg tccgagccga cgcgctcggc gcggatgacc
     2761 agcgtccccc ggccgttgat ggtcgcgccg gtcacgcgat cgcccggctt cttcgcgacc
     2821 ggcaccggct cgcccgtgat catcgactcg tcgatgttcg acgccccatc gacgcaggtg
     2881 ccgtcgaccg ggatcttgtc cccggggcgc acgcgcagcc ggtcgccgac cttcacctcg
     2941 gcgaggggca cctcgcgctc gcggccgtcg gcctcgatgc gcacggcggt gttgggcgcg
     3001 agcgcgagca gctcgcggat cgcgcggctg gtctggccga tcgcgcgcag ctgcatcacc
     3061 tcgccgagga gcaccagcgt gacgatcacc gcggcggcct cgaagtaggc gccgacctgc
     3121 ccgctctcct cctggaactc ctgcgggaac agcccgggcg cgaacaccgc cgcgacgctg
     3181 aagaggtacg cgagcgcgac gcccagcccg atcagcgtat acatgttggg gctgcggtgg
     3241 gcgagcgagc gccagaactt gacgaagaac ggccacccgc cccagagcac caccggtgtt
     3301 ccgagcgcga gctcgaggta catgcgcgcg tgctccagga tcccgaacgg ctggcggatc
     3361 ccgaagtagg gcgccatcgc gatcaccgcg agcggcgcgg tgagcgccgc gctcacccag
     3421 aaacgccgcg tcatgtggcg cagctcggtg tcgtcctcgc tcgcgcccgc catcgggacg
     3481 agcgccatgc cgcagatcgg gcagttgccc gggccttgcc gctcgacctc cgggtgcatc
     3541 gggcaggtat agagcgtccc cgcgggcgca ggcggatcgg cgcgcgcgtg cgcgggcgcg
     3601 gcgtagcggc cgggatcggc gacgaacttt tcgcggcagc gcgggttgca gaagtaccag
     3661 gtcttcccgc cgtgcacgtg cgagtgcttg gcggtcgcga tgtcgaccgt catgccgcag
     3721 acggggtcga ccgccttgcc gggtggcggc gtcgcgccgt cgccgtgcgc gtgcgaatga
     3781 tgcgtgttct cgtgggcggc gtgctccatc ggtcgtgtcc tagtgcggtt ggcgcacgcc
     3841 ggcaagcgcg cagaagccct gcgccgggac gtccagcgcg gcgttgaact tgctcgacat
     3901 gttctgatac gcgatcagcg cggtcagttc gatgatcgcg tcgtcgtcga aatggctctt
     3961 gagcgcggcg atcatggccg cgtccgtgcg ggtgcggctg tcggtgacgg cctcggcgta
     4021 gcgcagcgcc gccttttcgc gctcgctgaa cgcgccggag tcggcgtacc gggccagctc
     4081 ggcgagcttg cgcccgtccg cgccgcgccc gaggctgctc gccgcgttga tgtcgacgca
     4141 gaacgcgcag ccgttgatct gcgagacccg cacctgcacc agcgagcgca gcgccggctc
     4201 gatcggcgag gacttgcggt cgagcacgcc gtacatcaac gacatcgcga gaaaggcgcg
     4261 cggcagccgt ccccagaggc gcgaaggctc gagctcccgg ccgtagcgcc ggcgctgcag
     4321 cctcagaatc aggcgcacgt accagggaaa gcggtgtccg gccggtgttg ccacatggct
     4381 cacttgcgtc cctcctaggg cgaatttcga cattgtgctc ctcgacggca gggacgcgct
     4441 gccccacgcc agccacaggt acggatacat ggccgcaaac ggtcagtgga tcttgttgat
     4501 cacgtagtcc tgacctttct gcacgaactc gaaatcgacc ttgccgcccg ggtggacgtg
     4561 gtcgagcagc gccttgtcgc tcacgctgaa ggtcatggtc atcgccggcc atttgagggt
     4621 cccgatcgcg ccatgcgcga tggtgacctt cccggcggcg ggatcgactt tggtcacggt
     4681 gcccgagccc ttgtgcgtct tgccctgggc ggccgccgtc ttcggcgccg gcttcgcgcc
     4741 ctgacggtcg ttcatgtcca tcccgcccat gccgccgcct tgctgcgccc agctcgggtg
     4801 cgcagcggcg aggaacagga cgggagcgag aacagcgaga cgcttcattt cgtttctcct
     4861 ttcagctgcg attgaggacg cggtgcgcgc acggcgcggg cgcgggcccg cggccgccgc
     4921 atcaggtagt acgcgaccgg aacggcgaag agcgagatca gcggagcggt caccatgccg
     4981 ccgatcatcg gtgcggcgat gcgctgcatg acgtcgacac cggcgccatc ggacgccatg
     5041 atcggcagca ggccggcgag aatgaccgcc acggtcatcg ccttgggccg cacccggagc
     5101 gccgccccct cgtcgatggc ggacttcagc tccgcgtccc cgagcggctt gccgccggcc
     5161 aggcgctgct ccacggcgct cttcacgtag acgagcatca ccacgccgaa ctcggccgcg
     5221 acgccggcga gcgcgatgaa tcccacggcc gaggcgaccg acatgttgta gccgagcaga
     5281 tacatgagcc agaaaccccc gaccagcgag aagggcagtg tcgccatgat gaggagcgcg
     5341 tcggcgccgc tcctgaaggc cagatagagc agcacgaaga tgatcgcgag cgtcgccggc
     5401 accacgacct cgagccgcgc cgcggcccgc tccaggtatt cgaactgccc cgaccacgag
     5461 atcgagtagc cgggcggcag ctgcacttgc tcggcgaccg tccgcttcat ctcgcgcacc
     5521 gccgagctga gatcgcgccc ccggatatcc acgtacaccc agcccgagag ccgggcgttc
     5581 tcgctgcgga gcatcggcgg gccgtcggcg atccgcaccg ccgccacgtc gccgaggcgg
     5641 atctgcgcgc cgcgctcggt cagcatcggc aggctgcgga gcttctccac cgagtcccgg
     5701 atctcgcgcg ggtagcgcac gctgatcggg aagcgctgca ggccctcgac cgtctcgccg
     5761 atgttgtcgc cgccgatcgc cgccgacacg acgccgtgca cgtcggcgac gttcaggccg
     5821 tagcgcgccg ccgcgtcgcg gtcgatgtcc acgtcgatgt agcgcccgcc gcccaggcgc
     5881 tcggcaagcg ccgaggacac gcccggcacc tggcgcaccg cgcgctccac ctcgcgcgcc
     5941 aggcggtcga tctcggcgag ggaggcgccg gcgaccttca cgccgaccgg gctcttgatg
     6001 cccgtcgcca gcatgtcgat gcggttgcgg atcggcggca cccagatgtt cgacagcccg
     6061 ggaacctgta ccgtgcggtc gagctcctcg acgagccgct ccatggtcat tccggggcgc
     6121 cactgatcgc gcggcctgag ctggatggtg gtctcgaaca tctcgagcgg cgccggatcg
     6181 gttgcggtct ccgccctgcc cgccttgccg aagacgctcg cgacctcggg cacggtccgg
     6241 atcaggcgat cggtctgctg cagcagctcg gaggctttcc cgaccgatag accgggcagc
     6301 gccgtgggca tgtacagcag atcgccctcg tcgagcggcg gcatgaactc gccgcccatc
     6361 cgcgccatcg gccagagcgt cgccgcgagc aggacgagcg cgatggcgag cgcggtcttc
     6421 gggcgcgcga gcgccccggc gagcgccggc cggtagagcg cgatcagcga gcggctgatc
     6481 ggattgcgct gctcctcggg gatgcgcccg cggatcaggt agcccatcag caccgggatc
     6541 agcgtcaccg cgagaccggc ggccgcggcc atggcgtagg tcttggtgaa cgcgagcggc
     6601 gcgaacaggc gcccttcctg cgcctcgagc gtgagcaccg ggacgaacga gaacgtgatg
     6661 acgaggagcg aaacgaagag cgccggcccg acctgcgccg ccgcctctcc gatcacggcc
     6721 cagcgcgcct cgccgtcgag acgctcgccc gggtgcgcgt gcgcccaggc ttccagcttc
     6781 ttgtgcgcgt tctcgatcat caccaccgcc gcgtccacca tggcgccgat ggcgatcgcg
     6841 atgccgccca gcgacatgat gttcgcgttc acgccctggg tgtgcatcac gatgaaggaa
     6901 gcgaggatgc cgagcggcag cgagacgatc gcgacgaacg ccgagcgcag gtggaagagg
     6961 aacagcgcgc acacaagagc gacgacgacg aattcctcga gcagcttctg gccgaggttg
     7021 ccgaccgcgc gcttgatcag gctcgagcgg tcgtaggtcg ggacgatctc gacgccgggc
     7081 ggcaggctgg ggcgcagcga ctcgatcttc gccttcaccc cggcgatcgt ctccagcgcg
     7141 ttcttccccg agcgcatgag gacgatgccg cccgcgacct cgccctcgcc gttcagctcg
     7201 gcgatgccgc gtcgcatctc cggcccgacc tgcacctgcg cgacgtcgcc gaggcgcacc
     7261 ggcacgcccg cctccgtggt aacgagcggg atcttgcgga agtcgtcgag cgaacggagg
     7321 tagccgctcg cgcgcaccat gtactcggcc tcgcccagct cgagcaccga gccgccggtc
     7381 tcctggttcg cgcgctgcac cgcctcgacc accttggcct gcgggatgtt gaaggcgcga
     7441 aggcgcgccg gatcgagcac gatctggtac tggcgcacca tgccgcccac gctcgccacc
     7501 tcggccacgt tcggcacggc cttcagctcg tacttcagga accagtcctg cagcgcgcgc
     7561 agctgcgaca ggtccatgcg gcccgagcga tccacgagcg cgtactcgta cacccagccg
     7621 accccggtc
//
LOCUS       DATDMW010000374        11957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_857638, whole genome shotgun sequence.
ACCESSION   DATDMW010000374 DATDMW010000000
VERSION     DATDMW010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857638"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VI319_15650"
     CDS             complement(<1..409)
                     /locus_tag="VI319_15650"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEY6865329.1"
                     /translation="MNAPETLAPEAASPAGSDDFPDIGDYAAIGNCRTLALVSKAGAI
                     EWLCLPHFSGAAIFAATLDRGAGHFSVRPAGEFQVERAYVPATNVLATTFHTPGGTLR
                     LTDCMSLPPEPSGDLQSRVELEPEHEVLRLVECV"
     gene            complement(516..983)
                     /locus_tag="VI319_15655"
     CDS             complement(516..983)
                     /locus_tag="VI319_15655"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEY6865330.1"
                     /translation="MSESWSTRLTRWRFNLFPAYRGTGARIAYIRGDWSEVRVRLPLS
                     WRSRNYVGTIYGGSMYGAVDPVYMIMLMKRLGPGYTVWDKAASIRFRRPGRSTLYATF
                     AVGEDIVAAIRDELTRVPKLDRVFTVDLTDAAGTVHATVEKVLHVGRREPDPG"
     gene            complement(1005..1078)
                     /locus_tag="VI319_15660"
     tRNA            complement(1005..1078)
                     /locus_tag="VI319_15660"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1044..1046),aa:Cys,seq:gca)
     gene            complement(1143..1218)
                     /locus_tag="VI319_15665"
     tRNA            complement(1143..1218)
                     /locus_tag="VI319_15665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1183..1185),aa:Gly,seq:gcc)
     gene            complement(1235..1843)
                     /gene="pgsA"
                     /locus_tag="VI319_15670"
     CDS             complement(1235..1843)
                     /gene="pgsA"
                     /locus_tag="VI319_15670"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY6865331.1"
                     /translation="MLLNILNVPNILTFLRILMIPLVIGVFYFPETWLTGPEKNFTAT
                     ALFAAAALTDWLDGYLARKLNQMSAFGAFLDPVADKLMVASALIVLVWLDRLDPLIGI
                     IIIGREIAISALREWMARIGEAKSVAVNFLGKIKTISQMIAIPLLLFHERVGSLDAQR
                     AGTYLIWLAALLTLISMLYYLKLAAPGVERTLSGLTPGRRRP"
     gene            complement(1833..3641)
                     /gene="uvrC"
                     /locus_tag="VI319_15675"
     CDS             complement(1833..3641)
                     /gene="uvrC"
                     /locus_tag="VI319_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY6865332.1"
                     /translation="MTSSFDAAAFIAKLPSRAGVYRMLGASGEVLYVGKAGDLKKRVS
                     SYFQKSGHEPRIAHMIGQVAGVEITVTRSEAEALILENNLIKSMAPRYNILFRDDKSY
                     PYLMLSGGRFPRLGFHRGAMDKANRYYGPFPHAGAVRESIQLLQRVFRLRTCEDSVFQ
                     NRSRPCLLHQIRRCTAPCVGLVDEASYGEDVASASLFLEGRSDDVMKRLEARMQGAAD
                     ALRYEEAAAYRDQIQSLSRVSQKQYADTGAELDTDIVAVVDEHGLVCVNLVMVRGGRQ
                     LGDRSFFPQNAQGRERAEVLRAFVAQHYLERPIPPVLVLGEGGDAEDLERLLSEHAKR
                     HVRVVTRPIGERRAWLDAAHENARQALAQRLSEQATQEVRLAAMREALALPDTVQRIE
                     CFDVSHTMGEATVASCVVYDRKDMRRGEYRRYNIEGVTPGDDYAALAQALSRRYGRIA
                     GGEGVAPDLIFIDGGKGQVGAAKQALVETGLSDICLVGIAKGPERKPGLEELWVGERM
                     LQLPPDHPGLHLVQQIRDEAHRFAITGHRARRGKKRMTSTLEGIDGIGAKRRRQLLTR
                     FGGLKGVMGASIEDLAQVEGISRKLAEKIYQELHAA"
     gene            complement(3641..4318)
                     /locus_tag="VI319_15680"
     CDS             complement(3641..4318)
                     /locus_tag="VI319_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY6865333.1"
                     /translation="MTERLRRARPGYDPGCRDCPRLAAFLEAVRARHPAYHALPVAPF
                     GSARVRLLVVGLAPGMHGANRTGRPFTGDHAGLLLYRTLHRFGFASRPESAGAGDGLR
                     LIGCRITNAVKCLPPANKPLPEEVARCNRHLAAEIADLRPRALLALGAIAHGAVLRAL
                     ALRPSAFPFAHHAVHDLPLGPFVPDGARLFDSYHCSRYNTQTRRLTEAMFASVFRDLS
                     TFLNGAA"
     gene            4376..4942
                     /gene="efp"
                     /locus_tag="VI319_15685"
     CDS             4376..4942
                     /gene="efp"
                     /locus_tag="VI319_15685"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY6865334.1"
                     /translation="MAKILATEIRVGTLLEWEKRIWRVLKCYHVHVGGRGGAFMQVEM
                     KDIESGTKSNQRIRTEDKVERVSIDNREMQYLYHDGTHYVFMDKETFEQLQVSEDMLE
                     GQSGYLLPNTDVSVLFHNDRPIGVELPPSVVLTVTETDPNIKNATVTTSFKPAKTETG
                     LIVQVPPFVSTGEKIKVNTNDGAYIERA"
     gene            complement(4952..5881)
                     /locus_tag="VI319_15690"
     CDS             complement(4952..5881)
                     /locus_tag="VI319_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY6865335.1"
                     /translation="MAHDHDHHHDHDHGAHHEHGIRGAAFAIGIALNLAFVVVEAVYG
                     VAAHSMALLADAGHNLGDVLALGIAWAASALSRREPSVRFTYGLRSSSILAALFNAAL
                     LLLVTGGIAWEAIQRLAEPVDVSATTIVWVALAGILVNGTSATLFARHRHDLNARGAY
                     LHLVADAAVSAGTVLAGLGIIATGWMWLDPVTGLVIAAVIVAGTWTLLRDSVGLALDA
                     VPAHIDPSGVRDYLARLSGVTEVHDLHIWGMSTTETALTAHLVMPAGHPGDGYMAAVC
                     GELRERFRVQHATIQIETGDTAHPCPLASDRVV"
     gene            complement(5884..6894)
                     /gene="nagZ"
                     /locus_tag="VI319_15695"
     CDS             complement(5884..6894)
                     /gene="nagZ"
                     /locus_tag="VI319_15695"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY6865336.1"
                     /translation="MKLGRAMIDIAGLEPTAEEKALLSHPQVGGLILFARNFESPAQL
                     RRLAAEVRTLREPQLLIAVDHEGGRVQRFREGFTRIPPMRAIGARGRDDGARLARAAG
                     LVIAAELQAHGVDFSFAPVLDVDYGASSVIGDRAFCGDPAALAQLAGAFVEGLGAGGM
                     AAVGKHYPGHGYASADSHVDMPVDERELEAIEASDLVPYRRLIGQGLAAVMPAHVVYP
                     KVDGRPAGFSPVWLREILRARLGFEGMIFSDDLSMEAATQGGGVVERARAALAAGCDM
                     VLLCNAPDAAAKLIDGLDADPVPESRAARMRGRRLMRPAPAHAGYAQAVSALRRVIPE
                     AA"
     gene            complement(6891..7277)
                     /gene="acpS"
                     /locus_tag="VI319_15700"
     CDS             complement(6891..7277)
                     /gene="acpS"
                     /locus_tag="VI319_15700"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY6865337.1"
                     /translation="MILGIGTDLCEIGRIERALERWGERFAQRVLVESELQRFRRHRK
                     PAAYLAKRFAAKEAMSKALGTGIHYPVNWHGMWVENARSGKPSLRFSGPMAALLEKRG
                     IAAVHLSLTDETGMACAFVVIEGPGT"
     gene            complement(7274..8014)
                     /locus_tag="VI319_15705"
     CDS             complement(7274..8014)
                     /locus_tag="VI319_15705"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY6865338.1"
                     /translation="MTVLSVNLNKVALLRNTRTSGIPDVLKAARIALDAGCHGITVHP
                     RPDGRHVRGPDVRGLADLLRTRPGAEFNIEGNPFHGLLEFVREARPAQCTLVPDETGA
                     FTSDHGWDLARDRGRLGPAVAEARALGARVSLFMDPVPEAMRAAKQVGADRVELYTEP
                     YAQAFGGPDQAAVLARYAAAARAARDAGLGVNAGHDLNRDNLPAFLRAVPGIAEVSIG
                     HALIADALEFGLAETVRRYLAAIARGAE"
     gene            complement(8019..8729)
                     /gene="recO"
                     /locus_tag="VI319_15710"
     CDS             complement(8019..8729)
                     /gene="recO"
                     /locus_tag="VI319_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY6865339.1"
                     /translation="MTDAARQDGREAFVLHTYPYRETSLIVEAFTRTFGRVSLLARGA
                     RRPRSPMRGVLLSFQPLLVSWFGKGELRTMARAEWVGGQPLLGGQALLCGFYLNELLL
                     RLLPREDPHEALFAQYRLALQRLAAGDESGSVLRTFEKSLLKELGYAMVLDRDSAGGD
                     IDSSRTYRYDPERGPVEAAAGEPLVSGRTLLDMARDDYSDPVTQQQSKTLMRALINHR
                     LEHQPLRSRQVFRDLLQL"
     gene            complement(8722..9603)
                     /gene="era"
                     /locus_tag="VI319_15715"
     CDS             complement(8722..9603)
                     /gene="era"
                     /locus_tag="VI319_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY6865340.1"
                     /translation="MSAHRCGTIALVGRPNVGKSTLLNALVGQKLSITSRKPQTTRHR
                     IRGVLTLPDAQYVFVDTPGFQTRHRSMLNRLMNRGVKGALEDVDVAVLVTEAGRFGAE
                     DRAVLGLVPPAVRLFLVPNKIDRIGREALLPWLRKVATEAQFAEIVPVSAQKRKGLDG
                     LLRTLARYLPEQPPLYGEDDLTDRDERFFAAELVREKLFRLLGEELPYGASVAIDKFE
                     ETPGLRRIHASIVVDKDGHKAMVIGAKGEKLKAIGTAARADMEKLFGGKVYLEVWVKV
                     RRGWADSAAALKSLGYD"
     gene            complement(9600..10268)
                     /gene="rnc"
                     /locus_tag="VI319_15720"
     CDS             complement(9600..10268)
                     /gene="rnc"
                     /locus_tag="VI319_15720"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY6865341.1"
                     /translation="MKREGLDQRIGYTFARPELLAQALTHRSHGLPHNERLEFLGDGV
                     LNCVVAAELYERFGELKEGDLSRLRANLVRQETLCQLAHDIGVGDRLRLGEGEVRSGG
                     SDRPSILADALEALIGAVFVDGGFDAARALIRRLYAPLLAELDPRDSGKDPKTLLQEL
                     LQARKVALPQYSVVSTQGAAHEQQFEVECVIAELRLRTKGNGSSRRVAEQDAARRAFE
                     QLRG"
     gene            complement(10277..10636)
                     /locus_tag="VI319_15725"
     CDS             complement(10277..10636)
                     /locus_tag="VI319_15725"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEY6865342.1"
                     /translation="MRKQSGLGMIGVLFLCVAIVIGAIGGMKVVPAYLEYFNIKKAVK
                     GIVQSGQAAKSTPGEIRELFERRRAIDDFESVTGRDLQITKSGNDVEISYSYQRKIPL
                     FANVNLLIDFSGSSSQL"
     gene            complement(10669..11457)
                     /gene="lepB"
                     /locus_tag="VI319_15730"
     CDS             complement(10669..11457)
                     /gene="lepB"
                     /locus_tag="VI319_15730"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY6865343.1"
                     /translation="MNFALVLFVLTLFTGAFALADGVYFRRQRAPGQKEPWWIEYPKS
                     FFPVILIVFLLRSFLVEPFKIPSGSMVPTLLVGDFILVNKFAYGIRLPIVNKKIVGIG
                     EPKRGEVMVFRYPEDPSLDYIKRVVGLPGDRISYVNKKLTVNGVELPRKPLPDYLNRE
                     RIHYSKQYQEDLGGVEHSILIEDDVPAAIPFVRPFPFRDNCTYNNSGVTCTVPPGHYF
                     MMGDNRDNSADSRVWGFVPDENIVGKAFFIWFNFSEPSRFGRFR"
     gene            complement(11454..>11957)
                     /locus_tag="VI319_15735"
     CDS             complement(11454..>11957)
                     /locus_tag="VI319_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEY6865344.1"
                     /translation="NMQYLGRQVMLTYELPMAEVVMDFFDKLKSVSRGYASLDYDFKE
                     FRPADVVKLDILINGARVDALSVRVHRSVATYRGREVAARMRKLIPRQMFDVAVQASI
                     GAQIIARETIKAMRKNVLAKCYGGDITRKKKLLEKQKAGKKRMKQVGTVEIPQEAFLA
                     ILQVEHK"
BASE COUNT         1912 a         4129 c         4082 g         1834 t
ORIGIN      
        1 atacgcactc gaccaggcgc agcacctcgt gctcgggctc gagctcgacg cgcgactgca
       61 ggtcgcccga aggctcgggc ggcagcgaca tgcagtcggt gaggcgcagc gtgccgccgg
      121 gcgtgtggaa agtcgtcgcg agcacgttgg tcgcgggaac gtaggcccgc tcgacctgga
      181 actcgccagc cggccgcacc gagaagtgtc cggcgccccg gtccagggtc gcggcgaata
      241 tcgcggcgcc cgagaaatgc gggaggcaga gccactcgat ggccccggcc ttggagacga
      301 gcgccagcgt gcggcagttg ccgatggccg cgtagtcgcc gatgtcgggg aaatcgtcgg
      361 atccggcggg cgacgccgct tccggggcga gggtctcggg agcattcatg gagcgtgtcg
      421 gtctgacgcc gaagggcgcc ccattgttcc ggtttggccg ttaccgcgac accgcgccga
      481 tcgggagcgg gtagttcccc tgcaccatcc gggtactatc ccggatcggg ctcgcgccgg
      541 ccgacgtgga gcactttctc caccgtcgcg tggaccgtgc cggccgcgtc ggtgagatcg
      601 accgtgaaca cgcgatccaa cttcggcacg cgcgtgagct cgtcccggat cgccgcgacg
      661 atgtcctcgc cgaccgcgaa ggtcgcgtac agcgtgctgc ggcccgggcg gcggaagcgg
      721 atcgaggccg ccttgtccca cacggtgtaa ccgggaccga ggcgcttcat gagcatgatc
      781 atgtagacgg gatcgaccgc accgtacatc gagccgccgt agatcgtgcc gacgtagttg
      841 cgcgagcgcc acgacagcgg tagtcgaacc cggacctcgc tccagtcgcc gcggatgtac
      901 gcgatccgcg cgcccgtgcc ccggtacgcc ggaaacagat tgaacctcca gcgcgtcagg
      961 cgcgtggacc aggattccga catcgattca gcggccgagg cggatggagg cgggagtcgg
     1021 aatcgaaccg gcgtccacgg ctttgcaggc cgctgcatga ccactctgcc atcccgccgc
     1081 cgcgaggccg ttagaccccg aaaaagcaaa acgaagctgg gagcttcgtt ttaccgtgaa
     1141 tctggagcgg gagacgagtc tcgaactcgc gaccttaacc ttggcaaggt tacgctctac
     1201 caactgagct actcccgcga aacgaaagcg aattttacgg gcgccgcctc ccgggggtca
     1261 agccggagag cgtgcgctcc acgcccggcg cggcgagctt caggtaataa agcatggaga
     1321 tcaaggtcaa cagcgccgca agccagatca gatacgtgcc ggcccgctgg gcgtccaggg
     1381 agccgacccg ctcgtggaac agcagcaggg ggatcgcgat catctgggag atggtcttga
     1441 tcttgcccag gaagttcacc gcgacgctct tcgcctcgcc gatccgcgcc atccactcgc
     1501 gcagcgccga gatcgcgatc tcgcggccga tgatgatgat gccgatcagc gggtcgaggc
     1561 ggtcgagcca gacgagcacg atcagcgcgc tcgcgaccat cagcttgtcg gcaaccgggt
     1621 cgaggaacgc gccgaaggcc gacatctggt tgagcttgcg cgcgagatag ccgtcgagcc
     1681 agtcggtgag cgcggcggcc gcgaagagcg ccgtcgcggt gaagttcttc tccgggccgg
     1741 tgagccaggt ctcgggaaaa tagaacacgc cgatcaccaa cgggatcatc aggatccgca
     1801 ggaacgtgag gatgttcggg acgttgagga tgttaagcag catggagctc ctggtagatc
     1861 ttctcggcga gcttgcggct gatcccctcg acctgcgcca ggtcctcgat gctcgccccc
     1921 atcacgccct tcaggccgcc gaagcgcgtg agcagctgcc ggcgccgctt cgcgccgatg
     1981 ccgtcgattc cctcgagcgt cgaggtcatg cgcttcttcc cgcggcgcgc ccggtgtccc
     2041 gtgatcgcga agcggtgcgc ctcgtcgcgg atctgctgga ccaggtgcag ccccggatgg
     2101 tcgggcggca gctgcagcat acgctcgccg acccagagct cctccagccc gggcttgcgt
     2161 tcgggccctt tcgcgatgcc gacgaggcag atgtcggaga gcccggtctc gaccagcgcc
     2221 tgcttcgccg cgcccacctg ccctttgccg ccgtcgatga agatcaggtc gggcgcgacg
     2281 ccctctccgc ccgcgatgcg cccgtagcgg cgcgagagcg cctgcgcgag cgccgcgtag
     2341 tcgtcgccgg gcgtgacgcc ctcgatgttg taccgccggt actcgccgcg acgcatgtcc
     2401 ttgcggtcgt agaccacgca cgaggccacc gtcgcctcgc ccatcgtgtg gctcacgtcg
     2461 aagcactcga tgcgctgcac cgtgtcgggc agcgcgagcg cctcgcgcat cgccgcgagc
     2521 cgcacctcct gcgtcgcctg ctccgagagc cgctgcgcga gcgcctggcg cgcgttctcg
     2581 tgcgcggcgt cgagccaggc gcgccgctcg ccgatcgggc gcgtcacgac gcgcacgtgc
     2641 cgcttcgcgt gctccgagag caggcgctcg aggtcttccg cgtcgccgcc ctcgcccagc
     2701 accaggaccg gcgggatcgg ccgctcgagg tagtgctgcg cgacgaacgc gcgcagcacc
     2761 tcggcgcgct cgcgcccctg cgcgttctgc gggaagaagc tgcggtcgcc gagctgccgt
     2821 cccccgcgga ccatgaccag gttcacgcag acgaggccgt gctcgtcgac cacggcgacg
     2881 atgtcggtgt cgagctccgc tcccgtatcg gcatattgct tctggctgac gcgcgagagc
     2941 gactggatct ggtcgcgata ggccgccgcc tcctcgtagc gaagcgcatc cgcggcgccc
     3001 tgcatcctcg cctcgaggcg cttcatcacg tcgtcgctgc ggccctcgag gaagaggctc
     3061 gcgctcgcga cgtcctcgcc gtagctcgcc tcgtcgacca ggccgacgca gggcgcggtg
     3121 cagcgccgga tctggtgcag caggcacggg cgcgagcggt tctggaacac cgagtcctcg
     3181 caggtgcgca gccggaacac gcgctgcagc agctggatgc tctcgcgcac cgcgcccgcg
     3241 tgcgggaacg gtccgtagta gcggttcgcc ttgtccatgg cgccgcggtg gaatccgagc
     3301 cgcgggaacc tgccgccgct cagcatcagg tacgggtagg acttgtcgtc gcggaacagg
     3361 atgttgtacc gcggcgccat cgacttgatg agattgttct cgaggatcag cgcctcggcc
     3421 tccgagcgcg tcacggtgat ctcgacgccc gcgacctggc cgatcatgtg agcgatgcgc
     3481 ggctcgtgcc cggatttctg gaagtacgag gacacgcgct tcttgaggtc gcccgctttg
     3541 cccacgtaca gcacctcgcc cgaggctccg agcatgcggt agaccccggc gcgcgaaggc
     3601 agcttcgcga tgaaggccgc ggcatcgaac gacgaggtca tcacgcggcg ccgttgagga
     3661 aggtggacag gtcgcggaat accgacgcga acatcgcctc ggtgagccgc ctggtctgcg
     3721 tattatagcg gctgcagtga tagctatcga aaaggcgcgc cccgtcggga acgaacggcc
     3781 cgagcggcag gtcatgcact gcatgatgcg cgaacgggaa cgccgagggc ctgagcgcga
     3841 gcgccctgag aaccgcgccg tgggcgatcg cgccgagggc cagcaacgcg cgcggccgca
     3901 ggtcggcgat ctcggcggcg agatgccggt tgcagcgcgc gacttcctcg ggaagcggct
     3961 tgttcgcggg cggcaggcac ttcacggcgt tggtgatccg gcacccgatc agccgcaagc
     4021 cgtcgcccgc gcccgcggac tcgggccggc tcgcgaagcc gaagcggtgc aacgtgcggt
     4081 agagcagaag cccggcgtgg tcgccggtga acggccgtcc ggtgcggttc gccccgtgca
     4141 ttccaggggc gaggccgacc acgagcagcc gcactcgcgc ggacccgaag ggcgcgaccg
     4201 gaagcgcgtg ataggcggga tgccgcgcac ggaccgcctc gaggaaggcg gcgagcctcg
     4261 ggcagtcccg gcagcccgga tcgtaacccg ggcgggcgcg tcgaagacgt tccgtcaccg
     4321 gcccgattcg ttgtaaaatc ccgcgctttt gcgcacgtcc gcgaagcgaa ttcccatggc
     4381 caagatcctc gcaaccgaaa tccgcgtcgg aaccctgctc gaatgggaga agcgcatctg
     4441 gcgcgtgctg aaatgctatc acgtgcacgt cggcggccgc ggcggcgcct tcatgcaggt
     4501 cgagatgaag gacatcgaga gcggcaccaa gagcaaccag cgcatccgca cggaagacaa
     4561 ggtcgagcgc gtctcgatcg acaaccgcga gatgcagtac ctgtaccacg acgggacgca
     4621 ctacgtcttc atggacaagg agaccttcga gcagctccag gtctcggagg acatgctcga
     4681 gggacagagc ggctacctgc tgcccaacac cgacgtctcg gtgctcttcc acaacgaccg
     4741 cccgatcggc gtcgagctgc cgccctcggt ggtgctcacc gtgaccgaga ccgacccgaa
     4801 catcaagaac gcgaccgtca cgacctcgtt caagccggcg aagaccgaga cggggctcat
     4861 cgtccaggtg ccgcccttcg tcagcaccgg cgagaagatc aaggtcaata cgaacgacgg
     4921 cgcttacatc gaacgcgcgt agaggaccgc gtcaaacgac gcggtcggac gcgaggggac
     4981 aagggtgagc ggtatcgccc gtctcgatct ggatcgtcgc gtgctgcacg cggaagcgct
     5041 cgcgcagctc tccgcacacc gccgccatgt agccgtcccc cggatgcccc gcgggcatca
     5101 ccaggtgcgc cgtgagcgcc gtctccgtgg tgctcattcc ccagatatgc aggtcgtgga
     5161 cctcggtcac ccccgatagc cgcgccaggt agtcgcgcac gccgctggga tcgatgtgcg
     5221 cgggcacggc gtcgagcgcg agcccgacgg aatcgcgcag gagagtccac gtgcccgcga
     5281 cgatcacggc cgcgatgacg agcccggtca cgggatcgag ccacatccag cccgtcgcaa
     5341 tgatgccgag accggcgagc acggtgcccg ccgataccgc ggcgtcggcg acgaggtgca
     5401 ggtacgcgcc gcgcgcattg agatcgtgcc ggtgccgcgc gaacagcgtc gcgctggtgc
     5461 cgttcaccag gatcccggcg agcgccaccc agacgatggt cgtcgccgac acgtccaccg
     5521 gctcggcgag ccgctggatc gcctcccacg cgataccgcc cgtgacgagc agaagcagcg
     5581 ccgcgttgaa cagcgcggcg aggatcgagg agctgcgcag cccgtaggtg aagcgtaccg
     5641 agggctcgcg ccgcgagagt gcgctcgccg cccaggcgat tcccagcgcg agcacgtcgc
     5701 ccaggttgtg gccggcgtcc gcgagcaggg ccatcgaatg cgcggccacg ccatagacgg
     5761 cctcgaccac gacgaaggcg agattcagcg cgatgccgat cgcgaaggcg gcgccgcgaa
     5821 taccgtgctc gtggtgagcg ccgtggtcgt ggtcgtgatg gtggtcgtgg tcgtgcgcca
     5881 tgctcacgcc gcctcgggaa tgacgcgccg gagcgcggat acggcttgcg cgtagcctgc
     5941 gtgcgccggc gccggccgca tcagccgccg gccgcgcatg cgtgcggcgc gcgactcggg
     6001 caccgggtcg gcgtcgaggc cgtcgatcag cttcgccgcg gcgtccggcg cgttgcacag
     6061 gagcaccatg tcgcagccgg cggcgagggc cgcgcgggcg cgctcgacca cgcctccgcc
     6121 ctgcgtcgcc gcctccatcg aaagatcgtc gctgaagatc atcccttcga atccgaggcg
     6181 agcgcgcagg atctcgcgca gccacaccgg cgagaatccc gccggccggc cgtcgacctt
     6241 ggggtagacc acgtgggcgg gcatcacggc cgcgagcccc tgcccgatca gccggcgata
     6301 cgggaccagg tcgctcgcct cgatcgcctc gagctcgcgc tcgtcgaccg gcatgtcgac
     6361 gtgcgaatcg gcgctcgcgt agccgtgccc cgggtaatgc ttgccgaccg ccgccatgcc
     6421 gcccgcgccc aggccctcga cgaaggcgcc ggcgagttgc gcgagcgccg cgggatcgcc
     6481 gcagaacgcg cgatcgccga tgacgctgct cgccccgtaa tcgacgtcga gcacgggcgc
     6541 gaagctgaag tcgacgccgt gcgcctgcag ctcggcggcg atcacgaggc cggcggcgcg
     6601 ggcgagccgc gcgccgtcgt cgcgcccgcg cgcgccgatc gcgcgcatcg gcgggatgcg
     6661 cgtgaatcct tcgcggaagc gctgcacgcg gccgccttcg tggtcgaccg cgatcaggag
     6721 ctgcggctcg cgcagcgtgc gcacttccgc cgcgagcctg cgcagctgcg cgggcgactc
     6781 gaaattgcgc gcgaagagga tcagcccgcc gacctgcgga tgggacagaa gcgccttctc
     6841 ctcggcggtc ggctcgaggc cggcgatgtc gatcatcgca cgaccgagct tcacgttccg
     6901 ggcccctcga tgaccacgaa cgcgcacgcc atgccggtct cgtcggtcag cgacagatgc
     6961 acggcggcga ttccgcgctt ctcgagcagg gcggccatcg gcccggagaa ccgcagcgaa
     7021 ggcttccccg agcgcgcgtt ctcgacccac atgccgtgcc agttgaccgg gtaatggatg
     7081 ccggtgccga gcgccttgga catcgcctcc ttcgccgcga atcgcttcgc gaggtaggcc
     7141 gccggcttgc ggtgccggcg gaagcgctgc agctccgact cgaccagcac gcgctgcgcg
     7201 aagcgctcgc cccagcgctc gagggcccgc tcgatgcggc cgatctcgca caggtcggtg
     7261 ccgatgccga gaatcattcg gccccgcgcg cgatcgcggc taggtagcgc cgcacggtct
     7321 cggcgagccc gaactcgagc gcgtcggcga tcagcgcgtg gccgatcgaa acttccgcga
     7381 ttccgggcac ggcccgcagg aacgcgggaa ggttgtcccg gttgagatcg tgtccggcgt
     7441 tcacgccgag cccggcgtct cgcgccgcgc gcgcggccgc ggcgtagcgt gccagaacgg
     7501 cggcctggtc ggggcccccg aatgcctgcg cgtagggctc ggtgtagagc tcgacgcggt
     7561 ccgcgcccac ctgcttcgct gcgcgcatcg cctcgggaac cggatccatg aacaggctga
     7621 cccgcgcccc cagcgcgcgc gcttcggcga ccgccggccc caggcgaccg cgatccctgg
     7681 cgagatccca gccgtggtcg gaggtgaagg cgcccgtctc gtccggcacg agcgtgcact
     7741 gcgccggcct tgcctcgcga acgaactcga gcagcccgtg gaaggggttg ccctcgatgt
     7801 tgaactcggc gcccggccgg gtcctcagca ggtcggccaa gccgcgtacg tcgggtccgc
     7861 ggacatgccg tccgtcgggc cgcggatgca cggtgatgcc gtggcatccc gcgtcgagcg
     7921 cgatgcgggc agccttgagc acgtccggga tcccggacgt acgcgtgttg cgcagcaagg
     7981 ccaccttgtt gagattcacg ctgaggaccg tcatccgctc agagctgcag caggtcgcgg
     8041 aacacctggc ggcttcgcag cggctggtgc tcgaggcgat gattgatcag cgcccgcatg
     8101 agcgtcttgc tctgctgctg cgtcaccggg tcgctgtagt cgtcgcgcgc catgtcgagg
     8161 agcgtccggc cggacacgag cggctcgccc gccgcggcct cgaccggccc acgctcggga
     8221 tcatagcggt aagtccgcga cgaatcgatg tcgcctccgg cgctgtcccg atcgaggacc
     8281 atcgcgtatc cgagctcctt cagcagcgac ttctcgaagg tgcgcaggac cgagcccgac
     8341 tcatccccgg cggccaggcg ctgcagcgcg aggcggtact gagcgaacag ggcctcgtgc
     8401 ggatcctcgc gcggcagcag ccgcagcagc agctcgttca ggtagaagcc gcacagcagc
     8461 gcctggccgc cgaggagcgg ttggccgccc acccattcgg cgcgcgccat tgtgcgcagc
     8521 tcgcctttgc cgaaccacga cacgagcagc ggctggaacg acagcagcac gccgcgcatg
     8581 ggcgagcgcg gacggcgcgc cccgcgggcg agcaggctca cgcgtccgaa cgtccgggtg
     8641 aacgcctcga cgatgaggct ggtctcgcgg tagggatacg tgtgcagcac gaacgcttcg
     8701 cgcccgtcct gccgcgccgc gtcagtcata gccgaggctc ttcagcgccg cggcgctgtc
     8761 ggcccagccg cggcgcacct tgacccagac ttcgaggtaa accttgccgc cgaagagctt
     8821 ctccatgtcg gcgcgcgcgg ccgtgccgat cgccttcagc ttctcgccct tcgcgccgat
     8881 caccatggcc ttgtggccgt ccttgtcgac gacgatggag gcgtggatgc gccgcaggcc
     8941 gggagtctcc tcgaacttgt cgatcgcgac gctcgccccg tacggaagct cctcgccgag
     9001 caggcgaaac agcttctcgc gcaccagctc cgccgcgaag aagcgctcgt cgcggtcggt
     9061 caggtcgtcc tcgccgtaga ggggcggctg ctccgggagg tagcgcgcga gggtgcgcaa
     9121 cagcccgtcg aggcctttgc gcttctgcgc gctcacgggc acgatctcgg cgaactgcgc
     9181 ctcggtggcg accttgcgca gccacggcag gagcgcctcg cgcccgatgc ggtcgatctt
     9241 gttcggcacg aggaacagcc gcaccgcggg cggaaccagg cccagcacgg cgcggtcctc
     9301 ggcgccgaag cgccccgctt cggtcacgag cacggcgaca tcgacgtcct cgagggcccc
     9361 cttcacgccg cggttcatca ggcggttcag catgctgcgg tggcgcgtct ggaaccccgg
     9421 cgtgtcgacg aagacgtact gcgcgtccgg cagcgtcagc acgccgcgta tccggtgccg
     9481 cgtcgtctgc ggcttgcgcg aggtgatgct gagcttctgt ccgaccagcg cgttcagcag
     9541 cgtcgacttg cccacgttgg gccggccgac gagcgcgatc gttccgcagc gatgtgcgct
     9601 cacccgcgga gctgctcgaa ggccctgcgc gccgcatcct gctccgcgac gcggcggctg
     9661 ctgccgttgc ccttggtgcg caggcgcagc tcggcgatca cgcactcgac ctcgaactgc
     9721 tgctcgtgcg ccgcgccctg cgtggacacg accgagtact gcggaagggc gaccttgcgc
     9781 gcctgcagca gctcctggag cagcgtcttc ggatccttgc cggaatcccg gggatcgagc
     9841 tcggcgagca gcggcgcgta gagtcggcgg atcagcgccc gcgcggcatc gaagccgccg
     9901 tcgacgaaca ccgcgccgat cagcgcctcc aatgcgtcgg ccaggatcga aggccggtcg
     9961 gagccgccgc tgcgaacttc gccctcgccc aggcgcagcc ggtcgccgac gccgatgtcg
    10021 tgcgcgagct gacacagtgt ctcctgccgc acgaggttgg cgcgcaggcg cgacaaatcg
    10081 ccttccttga gctcgccgaa gcgctcgtac agctcggcgg cgacgacgca gttcaggacg
    10141 ccgtcgccga gaaactcgag gcgctcgttg tgcggcaagc cgtggctgcg gtgcgtgagc
    10201 gcctgtgcca ggagctccgg gcgcgcgaag gtgtagccga tgcgctgatc gaggccttcg
    10261 cgcttcattc cttccgctat agctggctgc tgctgcccga gaagtcgatc agcaggttga
    10321 cgttcgcgaa gagcgggatc tttcgctggt acgagtagga gatttccacg tcgttgcccg
    10381 acttggtgat ctgcaagtcc ctgccggtca cggattcgaa atcgtcgatc gcccgccggc
    10441 gctcgaacag ctcgcggatc tcgcccggcg tgctcttggc cgcctggccg ctctgcacga
    10501 tgcccttcac cgccttcttg atgttgaagt actcgaggta ggccggcacc actttcatcc
    10561 ccccgatcgc gccgatgacg atcgcgacgc acaggaacag taccccgatc attcccaagc
    10621 cggattgctt tctcatggtt ccccctgcgg atcgggtcga cacgcgcttc acctgaacct
    10681 tccgaaacgg ctgggctcgc tgaagttgaa ccagatgaag aacgccttgc cgacgatgtt
    10741 ctcgtccgga acgaatcccc agaccctgct gtccgcggaa ttgtcgcggt tgtcgcccat
    10801 catgaagtag tgccccggag gcactgtaca cgtcacaccg gagttattgt aggtgcaatt
    10861 gtcacgaaat gggaacggtc tgacgaacgg tatcgccgcc gggacgtcgt cctcgatcag
    10921 gatcgagtgc tcgacgccgc ccaggtcctc ctggtactgc ttcgagtaat ggatgcgctc
    10981 ccggttgagg tagtcgggca gcggcttgcg gggcagctcg acgccgttca ccgtcagctt
    11041 cttgttgacg tagctgatgc ggtcgccggg caggcccacc acgcgcttga tgtaatcgag
    11101 cgacggatcc tccgggtagc ggaacaccat gacctcgccg cgcttcggct cgccgatgcc
    11161 gacgatcttc ttgttgacga tgggcaggcg gatgccgtac gcgaacttgt tcaccaggat
    11221 gaagtcgccc accagcaggg tcggcaccat cgatcccgag gggatcttga acggctcgac
    11281 caggaacgat ctcagcagga acacgatcag gatgaccggg aagaagctct tggggtactc
    11341 gatccaccag ggctccttct ggccgggcgc gcgctgccgg cggaaataga cgccgtcggc
    11401 gagggcaaaa gcgcccgtga acagcgtcag cacgaacagg accagggcga agttcatttg
    11461 tgctccacct gcaggatggc gaggaacgcc tcctgcggaa tctccaccgt gccgacctgt
    11521 ttcatccgtt tcttgccggc cttttgcttc tcgaggagct tcttcttgcg ggtgatgtcg
    11581 ccgccgtagc atttcgcgag cacgttcttg cgcatcgcct tgatcgtctc acgggcgatg
    11641 atctgcgcgc cgatcgacgc ctgcaccgcg acgtcgaaca tctggcgcgg gatcagcttg
    11701 cgcatccggg ccgcgacctc gcggccgcgg taggtggcca ccgagcggtg caccctcacc
    11761 gagagcgcgt ccacgcgagc gccgttgatg aggatgtcga gcttcaccac gtcggccggg
    11821 cggaactcct tgaagtcgta gtcgagcgag gcgtagccgc gcgacaccga tttcaacttg
    11881 tcgaagaagt ccatcaccac ttccgccatc ggcagctcgt aggtgagcat gacctggcgc
    11941 ccgaggtact gcatgtt
//
LOCUS       DATDMW010000375         9541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2478967, whole genome shotgun sequence.
ACCESSION   DATDMW010000375 DATDMW010000000
VERSION     DATDMW010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2478967"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VI319_15740"
     CDS             <1..558
                     /locus_tag="VI319_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865345.1"
                     /translation="DRAMVACVLCEPFSAHKAYHWGVLADIAPALKVDGKYVANPLVE
                     TQRFADELGRIVYGDFKTGDAAKAGKALLAKGAVDLAALDEKIESICAKLLLTFPDCT
                     TKTIEELRKPKLEAWNRNKENHRAWLALNMMTEARAGFRAFNEGTKDDREVDFIALRQ
                     AIAQGEGWSDALTERIQPRAKRDGA"
     gene            555..1352
                     /locus_tag="VI319_15745"
     CDS             555..1352
                     /locus_tag="VI319_15745"
                     /EC_number="4.2.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexa-1,5-dienecarbonyl-CoA hydratase"
                     /protein_id="HEY6865346.1"
                     /translation="MSAQSTHGAAALGPVEIWLDRDESLLRIRLDRPKANIIDSAMIT
                     SLAQAFAAYANPGAVRAALIDAEGPHFSFGASVEEHLPARCAEMLAGLHELIRTMLEW
                     PRPILVAVRGQCLGGGLEVALAGNLIFASPDAQFGQPEIKLGVFAPAASCLLPGRVGP
                     AAAEDLLLSGRSIDAVEAKRLGLVHAVADDPEADALAYFDAHLAKKSASSLACAVQAA
                     RGAYLAEVELRLTQVERYYLNHLMQTRDANEGLAAFLAKRAPVWEHR"
     gene            1349..2521
                     /gene="bcrC"
                     /locus_tag="VI319_15750"
     CDS             1349..2521
                     /gene="bcrC"
                     /locus_tag="VI319_15750"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HEY6865347.1"
                     /translation="MSTTLEAIVARCESLFEDLRFEAVKQWKAAAPGRKAIGYMPVYV
                     PREIVHAAGMLPVGILGGGDQLEVIQGDAYYQSYICRIPRSTIELGLTGKLDCLDGML
                     FPSICDVIRNLSGMWQIMFKDKYVKYFDVPQNFQDGIGGDFYVHEMQLLREDLGKLAG
                     KPVTDDALRASIAVYNENRRAVRELYAYRAAKPWQAPTAEVYLVLRAGMVLPPEEHTQ
                     LVRDYIAAADAVERPKRDNARIVITGAFCEQPPLGLIKSIEMAGCYIVDDDFMLVLRW
                     LLDDVPLEGDPLENLSKAFLHRSATTASKYEEKKEEKGQFLLKSYKKAGAEGVIFSAP
                     SFCDPALLERPMLQAVLARYKVPYTAFKYAENTGQMAPIREQSGTFADSIKLWSAA"
     gene            2518..3879
                     /gene="bcrB"
                     /locus_tag="VI319_15755"
     CDS             2518..3879
                     /gene="bcrB"
                     /locus_tag="VI319_15755"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HEY6865348.1"
                     /translation="MSTPIQMMPPQPSKKPAPAVQKEDAMLLQKEMINRNYHELATAH
                     EHGRKVCATFVPGNLNELVMCFDMARSLPETNQLQNGLRKKSGKFIMDAERDGHSEDV
                     CTYVKSDLGMMLNGEVGPTGEPLPRPDLLLLSYTGCFTFMKWFELIRQKYGSDAVMLH
                     VPYQGDGRVSKNMRDYVVKQLKETVIPALERTSGVKFDIDRLRQYMKESVKAEEDLVH
                     VLQSAKNRPSPIDGYFGAVYYIGPIFTAFRGTPEATEYYRVLRGEIDERVRLKKGPVT
                     PEGDFGEEKYRLVVEGPPNWTSFRDFWKMFYEEGAVVVASTYAKVGGTYDFGFRHDPE
                     HPLESLAEYCLGCYTNLNFPQRIDMICKYIDEYQADGLLINSIKSCNSFSAGQLLILR
                     EVERRTGKPAAFVESDLVDPRYFSAANIKNRLESYFQMIKAKRGAASPTSSTPAFIPI
                     AAH"
     gene            3889..5226
                     /gene="bcrA"
                     /locus_tag="VI319_15760"
     CDS             3889..5226
                     /gene="bcrA"
                     /locus_tag="VI319_15760"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit A"
                     /protein_id="HEY6865349.1"
                     /translation="MRCFIGIDLGSTTTKAVVIDEGRNVLGRGITNSRSNYDAAATIA
                     KQEALLNARFHLFREALAKSGALNGGLDDFVGQLERDFRLEQFLEQLVDLHDTCRRNA
                     EGRFGERTTGVIAALEETFKRLEAEAPELFAPGAKRKSDFFRDIAGSRYLAVSEQVAK
                     EAGIPYEMLLNVFDRSIIEVENRVYDASIQPHLLAALGRTFRALPESAAREDAVRAPI
                     QGVLDTVLDETYVVGTGYGRARLPFSKEHIRSEILCHGLGAHVMYPATATVLDIGGQD
                     TKAIQVDPNGIVESFQMNDRCAAGCGRYLGYIADEMNMGLHELGPLAMKSTKTIRINS
                     TCTVFAGAELRDRLSLGDKREDIMAGLHRAIVLRAMSILARSGGIRDEFTFTGGVAKN
                     EAAVKALKALVTENYGEMTLNINPDSIYTGALGGATFAWRAVVEDGKQAEARQ"
     gene            5223..6065
                     /gene="bcrD"
                     /locus_tag="VI319_15765"
     CDS             5223..6065
                     /gene="bcrD"
                     /locus_tag="VI319_15765"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HEY6865350.1"
                     /translation="MIHTIGIDIGSGAVKATLFRVNGTRTDWIAKRVERIRRRDPISL
                     AREGYEAVLADAKMRPDDIDYVATTGEGENVKFATGHFYSMTTHARGGVYLRPDARAV
                     ADIGALNGRAIAVDERGKVLQYKMTSQCASGSGQFLENIARYLGVALEEIGPLSKSAS
                     NPEKVSSICAVLAETDVINMVSRGIATPDILKGIHISMASRLVRLLKVTGITQGTVLL
                     TGGLALDMGLLAALQEELVNEKITGLTAVSHPDSIYAGAIGAALWGAFRHDKLARLDE
                     RAAA"
     gene            6077..6319
                     /locus_tag="VI319_15770"
     CDS             6077..6319
                     /locus_tag="VI319_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEY6865351.1"
                     /translation="MALLINTDCTECDACVPVCPNEAIKAGTPYVIDPLRCTECVGAE
                     DEPQCKLVCPATCIVDHPDFRETREALLEKYQQIHG"
     gene            complement(6333..7367)
                     /locus_tag="VI319_15775"
     CDS             complement(6333..7367)
                     /locus_tag="VI319_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6865352.1"
                     /translation="MKLDIYNHFFPRRYFDRMVEVMPDARDMHKRVRAIPAIYDLDAR
                     LRVMDRFDDYAQILSLPSPPVESLGDPRVAAELARIGNDGLAELCRKHPERFEGFIAG
                     LAMNDPDAMLAEAGRAVKKLGAVGVQVFTNVNGAPLTASATLPLFDLMAELDLPIWIH
                     PARGADMADYKTEKKSHYEIWWTFGWPYETSVAMAHIVFAGLFDKHPGLKIITHHLGG
                     MIPYFEGRVGPGWDQLGTRTSDEDYGALLAKLKKRPLDYFRMFYADTAVFGSVAATEC
                     GLKFFGADRVLFASDMPFDPEKGSAYIRWTIDILDRLHVTPAERQAIYEGNARRLMRL
                     PARRKEPARV"
     gene            complement(7364..8404)
                     /locus_tag="VI319_15780"
     CDS             complement(7364..8404)
                     /locus_tag="VI319_15780"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6865353.1"
                     /translation="MNEIVLARSDAAVSVRALHESDFEQAVAIDAAIVFRSRRAYFER
                     RLEAASANPEQHVQLAAVQDGTLAGYLLARRMQGEFGRAEPGVRLETIGVRGGQQGRG
                     VGTRLMAALERWAAAHGVREIRTSAHWRDDAMLRFLDHAGFELGDRVLDRPVGPDGVA
                     PLERETGDEGGEGSDQRREANYGTQVLNDFTTLARDRVELCSLAPADLAGIESIDRSI
                     TGRDRGEYLARLVDEAVSGCGVRVSLVARRHGTIVGFLMARTDFGDFGRAEPIAVIDT
                     IGVHRWFAGAGIGAALLSQLFLNLNALGVQRVETVVALENLDLLAFFHKAGFAASGRL
                     AFVKPLNGGNRQ"
     gene            complement(8401..8925)
                     /locus_tag="VI319_15785"
     CDS             complement(8401..8925)
                     /locus_tag="VI319_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY6865354.1"
                     /translation="MESKSQTCRRLHDEHEAVFELLRRFGRTLAESAPGVTPDLSTAA
                     WAGLLRDVRGGLEGEVSRHFAFEERSLFPLLQEAGEGDLCDMFLEDHATVRGIATPLL
                     DLIAGAQAQAPARAAWESLKRLGLALCDALSEHALKEEQALLPALENILSEEQDRELF
                     AAYAGGDAVAHHQP"
     gene            complement(8938..>9541)
                     /locus_tag="VI319_15790"
     CDS             complement(8938..>9541)
                     /locus_tag="VI319_15790"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY6865355.1"
                     /translation="NQSDWWRGAKGYCLKHGGDTCHVAPQGKICRAAYSGDLAPALLA
                     LGAEVEIAGAGGARRKPLAELYADDGAAPLRLAAGDIVVAVHLPDAAPGLRSGYRKIR
                     PRGAIDFPLAGIAAALRTRSGRVESLRVAITGTNSRPFVLEGTADLAGAPLDDALFSA
                     LGKLVQHQVRPLRTTAASANYRRQVAVVLAQRLVRELTAI"
BASE COUNT         1560 a         3337 c         3273 g         1371 t
ORIGIN      
        1 gaccgcgcga tggtcgcgtg cgtgctgtgc gagccgttct cggcgcacaa ggcctaccac
       61 tggggcgtgc tcgccgacat cgcccccgcg ctgaaggtcg acggcaagta cgtcgccaac
      121 ccgctggtcg aaacgcagcg cttcgccgac gagctcggcc gcatcgtcta cggcgacttc
      181 aagaccggcg acgcggcgaa ggccggcaag gcgctgcttg cgaaaggcgc ggtcgacctc
      241 gccgcgctcg acgagaagat cgagtcgatc tgcgccaagc tgctgctcac cttccccgat
      301 tgcaccacca agacgatcga ggagttgcgc aagcccaagc tcgaggcctg gaaccgcaac
      361 aaggagaacc accgcgcctg gctcgcgctc aacatgatga ccgaggcgcg cgcggggttc
      421 cgcgccttca acgaaggcac gaaggacgac cgcgaggtcg atttcatcgc gctgcgccag
      481 gcgatcgcgc agggcgaggg ctggtccgac gcgcttaccg agcgtatcca gcccagggcg
      541 aagcgggacg gcgcgtgagc gcgcaatcca cgcacggcgc cgcggcgctc gggccggtcg
      601 agatctggct cgaccgcgac gagtcgctgc tgcgcatccg gctcgaccgg ccgaaggcga
      661 acatcatcga ctcggccatg atcacgtcgc tcgcgcaggc gttcgccgcg tacgcgaacc
      721 cgggcgcggt gcgcgccgcg ctgatcgacg ccgaaggccc gcatttcagc ttcggcgcga
      781 gcgtcgagga gcacctgccc gcgcgctgcg ccgaaatgct cgccggcctg cacgagctga
      841 tccggaccat gctcgaatgg ccgcggccga tcctggtcgc cgtgcgcggg cagtgcctgg
      901 gcggcggcct ggaggtcgcg ctcgccggca acctgatctt cgcgagtccc gacgcgcagt
      961 tcggccagcc cgagatcaag ctcggcgtgt tcgcgccggc ggcctcgtgc ctgttgcccg
     1021 ggcgcgtcgg gcccgcggcc gccgaggacc tgctgctctc gggccgctcg atcgacgccg
     1081 tcgaggcgaa gcgcctgggg ctcgtgcacg ccgtcgcgga cgatcccgag gccgacgcgc
     1141 tcgcctactt cgacgcccac ctcgcgaaga agagcgcgtc ctcgctcgcg tgcgccgtcc
     1201 aggccgcccg cggggcgtac ctcgccgagg tcgagctgcg cctcactcag gtcgagcgct
     1261 actaccttaa ccacctgatg cagacgcgcg acgcgaacga gggcctcgcc gccttcctcg
     1321 ccaagcgcgc ccccgtatgg gagcaccgat gagcacgaca ctggaggcaa tcgtcgcccg
     1381 ctgcgagtcg ctgttcgagg acctgcgctt cgaggcggtg aagcagtgga aggcggccgc
     1441 gccggggcgc aaggcgatcg gctacatgcc ggtctatgtg ccgcgcgaga tcgtgcacgc
     1501 ggccggaatg ctgccggtcg gcatcctcgg cggcggcgac cagctcgagg tgatccaggg
     1561 cgacgcctac taccagagct acatctgccg catcccgcgc tcgaccatcg agctggggct
     1621 gaccggcaag ctggactgcc tggacgggat gctgttcccc tcgatctgcg acgtgatccg
     1681 gaatctatcg ggcatgtggc agatcatgtt caaggacaag tacgtcaagt acttcgacgt
     1741 gccgcagaac ttccaggatg gcatcggcgg cgatttctac gtgcacgaga tgcagctgct
     1801 ccgcgaggat ctcggcaagc tcgccggaaa gcccgtgacg gacgacgcgc ttcgcgcctc
     1861 gatcgcggtc tacaacgaga accggcgcgc ggtgcgcgag ctgtacgcct accgcgcggc
     1921 gaagccctgg caggcgccca cggccgaggt ctacctggtg ctgcgcgccg gcatggtgct
     1981 gccgcccgag gagcacacgc agctcgtgcg cgactacatc gccgcggccg acgcggtcga
     2041 gcgcccgaag cgcgacaacg cgcgcatcgt gatcacgggc gcgttctgcg agcagccgcc
     2101 cctcggcctg atcaagtcga tcgagatggc gggctgctac atcgtcgacg acgacttcat
     2161 gctggtgctg cgctggctgc tcgacgacgt cccgctcgag ggcgacccgc tcgagaacct
     2221 gtcgaaagcc ttcctgcacc gctcggcgac gaccgcctcg aagtacgagg agaagaagga
     2281 ggagaagggc cagttcctcc tgaagtccta caagaaggcg ggcgccgagg gcgtgatctt
     2341 ctccgcgccg tcgttctgcg atccggcgct gctcgagcgc ccgatgctgc aggcggtgct
     2401 cgcgcgctac aaggtcccct acaccgcgtt caagtacgcg gagaacacgg ggcagatggc
     2461 gccgatccgc gagcagtccg gcaccttcgc cgactcgatc aaactgtgga gcgccgcatg
     2521 agcacgccga tccagatgat gccgcctcag ccttccaaga agcccgcccc cgcggtgcag
     2581 aaggaagatg cgatgctcct gcagaaggag atgatcaacc gcaactacca cgagctcgcc
     2641 accgcgcacg agcacgggcg caaggtctgc gcgacgttcg tccctgggaa cctgaacgag
     2701 ctggtgatgt gcttcgacat ggcgaggagc ctgcccgaga cgaaccagct gcagaacggc
     2761 ctgcggaaga aatccggcaa gttcatcatg gacgccgagc gcgacggcca ctccgaggac
     2821 gtctgcacct acgtcaagtc cgacctcggc atgatgctga acggcgaagt cggccccacg
     2881 ggcgagccgc tgccgcggcc cgacctgctg ctcctttcgt atacgggctg cttcaccttc
     2941 atgaagtggt tcgagctgat ccgccagaaa tacggctcgg acgcggtgat gctgcacgtt
     3001 ccctaccagg gcgacggccg cgtctcgaag aacatgcgcg actacgtcgt taagcagctg
     3061 aaggagaccg tgatcccggc gctggagcgc acctccggcg tgaagttcga catcgaccgc
     3121 ctgcgccagt acatgaagga gtcggtgaag gcggaggagg atctggtcca tgtgctgcag
     3181 tcggcgaaga accggccgtc gccgatcgac ggctacttcg gcgccgtcta ctacatcggg
     3241 ccgatcttca ccgcgttccg cggcacgccc gaggcgaccg agtactaccg cgtgctgcgt
     3301 ggcgagatcg acgagcgcgt gcgcctgaag aagggcccgg tcacgcccga gggcgacttc
     3361 ggcgaggaga aataccggct ggtcgtcgag gggccgccga actggaccag cttccgcgat
     3421 ttctggaaga tgttctacga ggagggcgcc gtggtcgtcg cctcgaccta cgccaaggtc
     3481 ggcggcacct acgacttcgg cttccgccac gaccccgagc atccgctcga gtcgctcgcc
     3541 gagtactgcc tgggctgcta caccaacctc aacttcccgc agcggatcga catgatctgc
     3601 aagtacatcg acgagtacca ggccgacggc ctgctgatca actcgatcaa aagctgcaac
     3661 agcttctccg cgggacagct cctcatcctg cgcgaggtcg agcgccgcac cggcaagccc
     3721 gcggccttcg tcgagtccga cctggtggac ccgcgctact tctcggcggc gaacatcaag
     3781 aaccggctgg agagctactt ccagatgatc aaggccaagc gcggcgccgc atcgccgacg
     3841 tcctcgacgc cggcgttcat cccgatcgcg gcccactagg agacgtccat gcgctgcttc
     3901 atcggcatcg acctcggctc gacgaccacc aaggcggtcg tgatcgacga gggccgcaac
     3961 gtcctcggcc gcgggatcac caactcgcgc tcgaactacg acgccgcggc gaccatcgcg
     4021 aagcaggagg cgctgctcaa cgcgcgcttc cacctgttcc gcgaggcgct cgcgaaatcg
     4081 ggggcgctga acggcggtct cgacgacttc gtcggccagc tcgagcgcga cttccgcctc
     4141 gagcagttcc tcgagcagct cgtcgacctg cacgacacct gccggcgcaa cgccgaggga
     4201 cgcttcggcg aacggacgac aggcgtgatc gccgcgctcg aggagacgtt caagcggctc
     4261 gaggcggaag cgcccgagct gttcgcgccg ggcgcgaagc gcaagtccga cttctttcgc
     4321 gacatcgccg gcagtcgcta cctcgccgtg agcgagcagg tggcgaagga ggcgggcatc
     4381 ccttacgaga tgctgctcaa cgtcttcgac cgctcgatca tcgaggtcga gaaccgcgtc
     4441 tacgacgcct cgatccagcc gcacctgctg gcggcgctcg ggcgcacgtt cagagcgctg
     4501 cccgagagcg ccgcgcgcga ggacgcggtg cgcgcgccga tccagggcgt gctggacacc
     4561 gtgctcgacg agacctatgt ggtcggcacg ggctacggcc gcgcgcgcct gcccttctcc
     4621 aaggagcaca tccgctccga gatcctgtgc cacgggctgg gcgcgcacgt gatgtacccg
     4681 gcgaccgcga ccgtgctcga tatcggcggg caggacacga aggccattca ggtcgacccg
     4741 aacggcatcg tcgagagctt ccagatgaac gaccgctgcg ccgcgggctg cggccgctat
     4801 ctcggctaca tcgccgacga gatgaacatg ggcctgcacg agctcgggcc gctcgcgatg
     4861 aagtccacga agacgatccg catcaactcg acctgcaccg tgttcgcggg cgcggagctc
     4921 cgcgaccggc tgtcgctcgg cgacaagcgc gaggacatca tggcggggct gcaccgcgcc
     4981 atcgtgctgc gcgcgatgtc gatcctcgcg cgctcgggcg gcatccgcga cgagttcacc
     5041 ttcaccggcg gcgtcgccaa gaacgaggcc gcggtgaagg cgctgaaggc gctggtcacc
     5101 gagaactacg gcgagatgac gctcaacatc aaccccgact cgatctacac gggagcgctc
     5161 ggcggcgcga cgttcgcctg gcgcgcggtg gtcgaggacg gcaagcaggc ggaggcgcgg
     5221 caatgatcca tacgatcggc atcgacatcg gctcgggagc ggtcaaggcg acgcttttcc
     5281 gggtgaacgg gaccaggacc gactggatcg cgaagcgcgt ggagcgcatc cgccgccgcg
     5341 acccgatctc gctcgcgcgc gagggctacg aggcggtgct cgccgacgcg aaaatgcggc
     5401 cggacgacat cgattacgtg gcgacgaccg gcgagggcga gaacgtgaag ttcgcgaccg
     5461 ggcatttcta ctcgatgacc acgcacgccc gcgggggcgt ctacctccgg cccgacgctc
     5521 gcgcggtggc cgacatcggc gcgttgaacg gccgcgcgat cgccgtcgac gagcgcggca
     5581 aggtgctgca gtacaagatg accagccagt gcgcctccgg cagcggccag ttcctggaga
     5641 acatcgcgcg ctacctcggc gtcgcgctcg aggagatcgg accgctctcg aagagcgcgt
     5701 ccaaccccga gaaggtcagc tcgatctgcg ccgtgctcgc cgagaccgac gtcatcaaca
     5761 tggtgtcgcg cgggatcgcg acacccgata tcctcaaagg gatccacatc tcgatggcgt
     5821 cgcgcctggt gcggctgctc aaggtcacgg gcatcacgca gggcacggtg ctgctcaccg
     5881 gcggcctcgc gctcgacatg ggcctgctcg ccgcgctcca ggaggagctc gtcaacgaga
     5941 agatcaccgg gctgaccgcc gtcagccatc ccgactcgat ctacgccgga gcgatcggcg
     6001 cggccctctg gggcgcgttc cggcacgaca agctcgcgcg actcgacgag cgcgcggccg
     6061 cctgaaggag gaagccatgg ccctgctgat caacaccgac tgcaccgagt gcgatgcctg
     6121 cgtcccggtg tgcccgaacg aagccatcaa ggcgggaacg ccgtacgtga tcgacccgct
     6181 gcgctgcacc gagtgcgtcg gcgccgagga cgagccgcag tgcaagctcg tgtgcccggc
     6241 gacgtgcatc gtcgaccacc ccgacttccg cgagacgcgc gaggcgctgc tcgagaagta
     6301 ccagcagatc cacggctgac ccgtccggcg ggtcagacgc gagccggctc tttccgccgc
     6361 gcaggcagcc tcatcagccg gcgcgcattc ccctcgtaga tcgcctggcg ctccgccggc
     6421 gtgacgtgca gccggtcgag gatgtcgatc gtccagcgga tgtaggccga gcccttctcc
     6481 gggtcgaacg gcatgtcgga ggcgaacagc acgcggtcgg cgccgaagaa cttcaggccg
     6541 cactcggtcg cggccaccga cccgaagacc gcggtgtcgg cgtagaacat ccggaagtag
     6601 tcgagcgggc gcttcttcag cttcgcgagc agcgcgccgt agtcctcgtc cgaggtgcgc
     6661 gtgccgagct ggtcccagcc cgggccgacg cggccctcga agtaggggat catgccgccc
     6721 aggtggtggg tgatgatctt caggcccggg tgcttgtcga agaggcccgc gaacacgatg
     6781 tgcgccatcg cgacgctggt ctcgtacggc cagccgaagg tccaccagat ctcgtaatgg
     6841 ctcttcttct cggtcttgta gtcggccatg tcggcgccgc gagcggggtg gatccagatc
     6901 ggcaggtcga gctcggccat gaggtcgaag agcggcagcg tggcgctcgc cgtgagcggc
     6961 gcgccgttca cgttcgtgaa cacctgcacg ccgacggcgc ccagcttctt caccgcgcgc
     7021 ccggcctcgg cgagcatcgc gtcggggtcg ttcatcgcga gtcccgcgat gaagccctcg
     7081 aagcgctcgg ggtgcttgcg gcacagctcc gccaggccgt cgttgccgat gcgggcgagc
     7141 tccgcggcga cccgcggatc cccgagcgac tcgaccgggg ggctcggcag cgacaggatc
     7201 tgcgcgtagt cgtcgaacct gtccatgacg cgcaggcgcg cgtcgagatc gtagatcgcc
     7261 gggatcgcgc gcacgcgctt gtgcatgtcg cgcgcgtcgg gcatgacctc gaccatgcgg
     7321 tcgaagtagc gcctgggaaa gaaatggttg tagatgtcga gcttcactgg cggttccctc
     7381 cgttgagtgg cttcacgaac gcgagccggc cgctcgcggc gaaccccgcc ttgtggaaga
     7441 aggccagcag gtcgaggttc tccagcgcga cgacggtctc gacgcgctgg acgcccagcg
     7501 cattgagatt cagaaacagc tgggacagca atgcggcgcc gatccccgcg ccggcgaacc
     7561 agcggtgcac gccgatcgtg tcgatgacgg cgatcggctc ggcgcggccg aagtcgccga
     7621 agtcggtcct cgccatgagg aagccgacga tggtgccgtg gcgcctcgcc acgagcgaga
     7681 cgcgcacgcc gcaacccgac accgcctcgt cgacgagtcg cgcgaggtac tcgccgcggt
     7741 cgcgccccgt gatgctgcgg tcgatcgact cgatgccggc gagatcggcc ggcgcgagcg
     7801 agcacagctc gacgcggtcg cgcgcgagcg tggtgaaatc gttcagcacc tgcgtgccgt
     7861 agttcgcctc gcggcgctgg tcgctgccct cgccgccctc gtcgcccgtc tcgcgctcga
     7921 gcggcgcgac gccgtcgggg ccgaccggcc ggtcgagaac gcggtcgccc agctcgaagc
     7981 ccgcgtggtc gaggaatcgg agcatcgcgt cgtcgcgcca gtgggcgctg gtgcggatct
     8041 cgcgcacgcc gtgcgccgcg gcccagcgct cgagcgccgc catgaggcgc gtgcccacgc
     8101 cgcgcccctg ctgcccgccg cgcacgccga tggtctcgag ccgcacgccg ggctcggcgc
     8161 ggccgaattc gccctgcatc cgccgcgcga gcaggtagcc cgcgagcgtc ccgtcctgga
     8221 ccgcggcgag ctgcacatgc tgctcggggt tcgccgaagc cgcctcgagc cgccgctcga
     8281 aataggcgcg ccgcgagcgg aagacgatgg cggcatcgat cgcgaccgcc tgctcgaagt
     8341 cgctctcgtg cagcgcacgc accgacaccg cggcgtccga ccgggccagc acgatctcgt
     8401 tcatggctgg tggtgcgcga ccgcgtcgcc gccggcgtag gcggcgaaca gctcgcggtc
     8461 ctgctcctcg gaaaggatgt tctcgagcgc aggcagcagc gcctgctcct ccttgagcgc
     8521 atgctcggat agcgcgtcgc acagcgcgag gccgaggcgc ttcagcgatt cccaggccgc
     8581 gcgcgcgggc gcctgcgcct gcgcgcccgc gatcaggtcg agcagcggcg tcgcgatccc
     8641 gcgcaccgtc gcgtggtctt ccaggaacat gtcgcacagg tcgccttcgc ccgcctcctg
     8701 gaggagcggg aagagcgagc gctcttcgaa cgcaaaatgc cggctgacct cgccctcgag
     8761 tccaccgcgc acgtcgcgca gcaggccggc ccacgccgcg gtcgacagat cgggcgtcac
     8821 cccgggcgcg ctctcggcga gcgtgcggcc gaagcggcgc agcagctcga acaccgcctc
     8881 gtgctcgtcg tgcaggcggc ggcaggtctg gctcttcgat tccacgtcgt tctccttcta
     8941 gatggccgtc agctcgcgca ccaggcgctg cgccagcacc acggcgacct ggcgccggta
     9001 gttcgcgctc gccgcggtgg tccgcagcgg gcgcacctgg tgctggacca gcttgccgag
     9061 cgcagagaac agcgcgtcgt cgagcggggc cccggcgagg tcggcggtgc cctcgagcac
     9121 gaacgggcgc gagttggtcc cggtgatcgc gacgcgcagc gactcgacgc ggcccgagcg
     9181 agtgcgcagg gcggcggcga tgccggcgag cgggaaatcg atcgcgccgc gcggccggat
     9241 cttgcggtat cccgagcgca gccccggcgc ggcgtcggga aggtgcaccg cgacgacgat
     9301 gtcgccggcc gcgagccgca agggcgcggc cccgtcgtcg gcgtacagct cggcgagcgg
     9361 cttccgccgc gcgccgccgg cgcccgcgat ctcgacctcc gcgccgagcg cgagcagcgc
     9421 gggcgcgaga tcgccgctgt acgcggcgcg gcagatcttc ccttgcggcg ccacgtggca
     9481 ggtgtcgccg ccgtgcttga ggcaatagcc tttcgcgccg cgccaccagt cgctctggtt
     9541 g
//
LOCUS       DATDMW010000376        25075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1231531, whole genome shotgun sequence.
ACCESSION   DATDMW010000376 DATDMW010000000
VERSION     DATDMW010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25075
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231531"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1285
                     /locus_tag="VI319_15795"
     CDS             143..1285
                     /locus_tag="VI319_15795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6865356.1"
                     /translation="MRALSMSMLLAAATLVAGCGDHDQKTEAPPPRLEDGRVVFSEGN
                     PQLAALQSDVVKPGAPEQLKLTGRLVWDEERTVRLFPPFAGRVARILVKPGDVVKAGE
                     PLALLFSPDFGQAQAEARKAQTDFALAKKSLERLRELNTAGVAARKDVNAAEADYARA
                     EAELARAEGRVKLYGASGDTVDQSFALRSPISGTVVERNINPGQELRPDVAAGAPAMF
                     VVTDPSRLWVQLDANERDISRLKRGLHATLRTGAWPEETFPATVVAVADFIDPATRTV
                     KLRGIVDNRARKLKGEMFVSAEMEGAPRAQVQVSEKAIVQANGRNYVFIEEAPGRFAR
                     VQVDVEGVRDGTAGVVSGLTQGQKVVVEGNLFLNRLYRQLSGDAAA"
     gene            1295..4381
                     /locus_tag="VI319_15800"
     CDS             1295..4381
                     /locus_tag="VI319_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY6865357.1"
                     /translation="MKRIVSFALHKPLFIVLGLILFIGSGLAAFRSLPIEAFPDVTDT
                     QVNVIALYPGRAAEEVEKQVTVPLEVALAGLPNAVRMFSHTQFGLSFIIITFDDRANP
                     YFARQQVVERLAAVDMPPGVQPQLAPLSTPIGEIYRYRLSSDSHMDPRDLRTLEDWTV
                     ERQLRLIPGVADIVTIGGLIKQYEVNPDLARLRDYKITLQQLFAALDRGNANVGGSYL
                     EQGRQQYLIRGIGLLRSADDIGNIVVAERNGVPILIKNLADVSIGAVPRQGVVGQDRD
                     DDIVTGVVLMRKGENPSEVLKAVKAKVGQLNASLLPPGVKIVPFYDRTWLIEHTLKTV
                     FSNLTEGAALVTLVLLLFLGNLRAALIVALVIPLSLLATFLGLTFVGIPANLLSLGAM
                     DFGIIVDGAVIVVENVFRKLSDPGHERTPRLQRILDATIEVGRPTLFSMLIIIAAHIP
                     IFTLQRHEGRIFAPMAWSVTSALVGSLILSLTLVPLLCLHFLPKHLPEEENRLVRACK
                     RLYEPALVWAVGRKKIVIGIALAALAASFAVVPKLGTEFLPELNEGSIWINVTLPSSV
                     SVTEAQAMTGKFRAVLAKIPEVDTVISKSGRPEDGTDPKLINMCEMLVVPKPESEWRP
                     GMTKRKLVDQMDDAMSVFPGIEPSFSQPIRDNVLESISQIDGQIVIKVFGDDLDVLSE
                     QAQAVLHAISDVRGVKRAFIDRLGELPQLLIRIDREAAARYGLNVADIQDVIEMVLGG
                     KPATQLWEGERHFAVTVRLKDAQRTIPNLEAITVATPGGAYVPLSQVAEFKTVGGAMN
                     IAREDGVRVYAIGVFIADRDMGSVVADMKDRVAAQVKLKPGYRVTWSGEFENQERAMA
                     RLAIIIPVSVLLIFLLLFDAFGRVSSATLILLNIPFALIGGIFALLVTGIPLSVSAAI
                     GFIALFGQAVLNGVVMVAYFNQLRASGMPPEQAVIKGSLVRMRTVLMTGLLAMLGLLP
                     MAISTGIGSETQKPLAVVVIGGLVSATLLTLIVLPVLYLVFQRHHHARPAAGGQPH"
     gene            4483..4695
                     /gene="rpsU"
                     /locus_tag="VI319_15805"
     CDS             4483..4695
                     /gene="rpsU"
                     /locus_tag="VI319_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY6865358.1"
                     /translation="MPTVRVKENEPFEVALRRFKRTVEKTGLLTELRAREFYEKPTAY
                     RKRKLAAAIKRHYKRLRNQTLPPKLY"
     gene            4785..5231
                     /locus_tag="VI319_15810"
     CDS             4785..5231
                     /locus_tag="VI319_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY6865359.1"
                     /translation="MPLKERIQEDMKAAMKARETQRLSAIRLLLAAIKQREVDERKDL
                     SDPEIASVIEKMIKQRRDSIAQYTSANRTDLADAETFELNLLAGYLPKQLSDAEIAKA
                     VDDALAQTGAKGAADMGKVMGALKAKLAGRADMAKVSALVKAKLAG"
     gene            5290..7086
                     /gene="dnaG"
                     /locus_tag="VI319_15815"
     CDS             5290..7086
                     /gene="dnaG"
                     /locus_tag="VI319_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY6865360.1"
                     /translation="MIPDSFKQELLHRVDIVDVIERHMTLKKAGANFAACCPFHSEKT
                     PSFTVSPTKQFYHCFGCGAHGNAISFLMEYSGLGYVDAVRELAEDVGMKMPEFEPRAK
                     KEETGPDLHPVMQRACDYYREQLKASPRAIDYLKHRGLTGKVAARFGIGYAPPGWQNL
                     QSVFPGYADKALKDAGLVIDGEAGRRYDRFRDRIMFPIQDQRGSVIGFGGRVIEPPLD
                     DERDPGPKYLNSPETPLFQKGQELYGLPQARQAIRAAGRVLVVEGYMDVVALAQHGVE
                     YAVATLGTATSATHVQRLLRQTDEIVFSFDGDAAGRNAAWKALEVSLPAVTDTKVVRF
                     LFLPPEHDPDSFVRERGVQAFEEAVGKAEPLAQFFAEGLKERLGTGAVMEGKSHFFHQ
                     AKAFLQPLAAINLKQNLLKHVAQIYRISEEEAARDLGISIARTPASRYGHVPAREERA
                     PMQSLEVWLLKRLMVKPSLAAEMPAELLRGQSFESLALQVFVAFARETAGANFDALVI
                     DRFHGTEFEGLFKQIKKEVMVMALSPEDVEMEFRDALPNMELQHVKDRLAELVRQAEL
                     RRLTPEEEAQLARLAARRKELDVVRSGSPPVL"
     gene            7126..9561
                     /gene="rpoD"
                     /locus_tag="VI319_15820"
     CDS             7126..9561
                     /gene="rpoD"
                     /locus_tag="VI319_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY6865361.1"
                     /translation="MSKQRLKSKAKARPAGRAKGKIGLKSPRAREMDKLKAQREKAAA
                     KLRAQKERERDRMQRERDRERARKEKEAAHAKAAKERERARREKEAQKARERTEKERE
                     RLRLQKEKLRERERVAKEKAKAKELAAREKAKAKELAAQEKAKAKEAAALQKQKEREL
                     KAQLKMQAQELMKLRKAGRGSRRNGDAERALLLRAEARPEDAEARRTRLKNLIKLGKE
                     RGFLTYAEINDHLPDDMVDAEQIESIITTFNDMGIQVYDEAPDAEQLLISENAPPVVP
                     DEDAEEEAEAALSTVDPEFGRTTDPVRMYMREMGSVELLTREGEIEIAKRIEDGLKHM
                     IQAISACPTTIAEILELSAKVARDEMRIDELVDGLIDPNAKEEPITELADDVEPEEEE
                     EGEGEEDSAALSASLLQLKEDALARFAKIQRLYEKMKKALVEHGHRSKEYIRVRDQIG
                     GELTNIRFTARIIERLCDSLRGMVDEVRGHEREIQALCVMKSNMPRPHFIKVFPGNET
                     NMRWVKNEIAATRPWSSVLTRYEPAILEQQQKLLAIQKRIGIPLKDLKEINKQMSTGE
                     AKARRAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKF
                     STYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQETGQEPDPATLAQ
                     KMEMPEEKIRKILKISKEPISMETPIGDDDDSHLGDFIEDQATLAPNDAAMYASLREV
                     TKDILDSLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHP
                     SRSERLKSFLDNS"
     gene            9642..9718
                     /locus_tag="VI319_15825"
     tRNA            9642..9718
                     /locus_tag="VI319_15825"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9676..9678,aa:Ile,seq:cat)
     gene            complement(10045..10671)
                     /locus_tag="VI319_15830"
     CDS             complement(10045..10671)
                     /locus_tag="VI319_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865362.1"
                     /translation="MFKNEFMQTLLFPSVVEFFLLVGVVGVLVGIGLILWREPTLRFL
                     AVLNRWVSTRRWLKSAEIPHDTSSGVQRYRLWIGLFFVLAAAYSLFGLLTRFNVHAVL
                     PANSLGGWTPAAHWLLDSLRWFLVVGSIAAAAVGALLVLMPETLRKLEARANEWHSSR
                     KAFGGVADAMYMPLDKWVEHYPRLAGAVIAAGAFAVAIGSVMVLARMK"
     gene            10774..11736
                     /locus_tag="VI319_15835"
     CDS             10774..11736
                     /locus_tag="VI319_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865363.1"
                     /translation="MRARNAAVFLCAALAWGTALGQAWPSKPIHYLVPFPPAGATDIT
                     ARIIADKITGPLGQPVIVENRPGAAGNVGSEVVAKSTPDGYTVLQCTVAQSISQTLYA
                     KLNYDLEKDLAPVAMIALVPNVMEVNPSVPAKSVAEFIALAKSKPGKINFASSGSGTS
                     IHMSAEMFMMLTGVKMVHVPYKGSGPALAGMLGGEVDVMFDNLTSSIAYIKSGKLRAL
                     AVTTATRYPELPDVPTMQEAGVAGYEATAWFGMLAPKGTPRSVVARLNAEVNKALAQP
                     DVKEKLAQQGAMASPWTPEQFGEFIHNEIAKWGKVVKASGAKVE"
     gene            complement(11744..12571)
                     /locus_tag="VI319_15840"
     CDS             complement(11744..12571)
                     /locus_tag="VI319_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6865364.1"
                     /translation="MSAGAPADRTVDVGGLRTRYIERGAGPAALLLHGASLGSSADVW
                     TGNLDALAAHGLRVIAFDQPGFGLSDDADDHSVGFRVRFVPAFMDALGLERAHLVGHS
                     QSGRFAVSLALSHPGRVARAVVVATGSLLPPLPGAGAPGEGDEGEAAEPTLEDTRAAL
                     EATVFHRELVTPEAVALRHRMSTGRNFRAFLARRQARGAGEKKDAAPLWQRLGEVRVP
                     LRLIYGADDRGDCARRTRLALERFPGLDLHLVERCKHLVQWDAPERFAELTGRFLAS"
     gene            complement(12568..13371)
                     /locus_tag="VI319_15845"
     CDS             complement(12568..13371)
                     /locus_tag="VI319_15845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6865365.1"
                     /translation="MSARIRHIAIVSEQYALEGRFYEAIFGMRPASDTRPERAVTVSD
                     GYVGLNINPRKAGRGAGLDHFGLEVDDADAVRARLRERYPAIQVLARPSTRPFAGVST
                     HDPDGNIFDLSQRSMENRASVYVEAEREQARAISHIAIRTLHPERTAEFYTDVFGLEP
                     SARKAPDGSLALSDGRVALVLMPWSILDYHGGGIARPGPDHIGFKVESIAALEQHIAK
                     VGANNPHLRPLPVGAGSEGEARLKLFASCPHGRYHLADPDGVLIDVAER"
     gene            complement(13374..14381)
                     /locus_tag="VI319_15850"
     CDS             complement(13374..14381)
                     /locus_tag="VI319_15850"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6865366.1"
                     /translation="MTPNRRTLLASLAAAGLLVLAPAAAQSIDAARNERAVVWYTAMT
                     TTDAEPLRDALQARYPFLQLTILRQPGEKIRTRILTEARAGKHLWDVVSFNHLDMDAL
                     DREELLAAYASPETRSGFPPGAVDPRGRWAAIYVRQFVIGYNLRAVPPGQAPRSWQDL
                     LEPRWKGKFAMDEDDSEWYAAMLDYLGRERGTAFMQALARQQPQLRRGHSLLSKLLVA
                     GDFPLALVHAAEVEGEKKTGAPVDWVRSLDPIITSPSQVAVAAGAPHPNAARLLVDLL
                     LSVEGQTIIRSRGRVPARTDLPGAGPDESRLRIHYVDPKLARERDRSEREFRDIFLKA
                     R"
     gene            14470..14856
                     /locus_tag="VI319_15855"
     CDS             14470..14856
                     /locus_tag="VI319_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865367.1"
                     /translation="MPRSALSGLGLLLAILIAPPVVAQAPVEERQRRTDSYTEAQQRV
                     EFSRQGAERAARRVKDAEQGLKDAEAELGPAQRRYEDSKSRVERARTELKQARAVESE
                     SRKTYEAESAAFQRLRARGNDTKGTK"
     gene            14853..15230
                     /locus_tag="VI319_15860"
     CDS             14853..15230
                     /locus_tag="VI319_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865368.1"
                     /translation="MNLRLMAAVPGLAVALAVPCARAQQDGQPEAPKVVTKTLVKSEA
                     QKECFKLVNAQVLYYRFNADGPLDFKLSHQEDKEIVHIARTGIEDAAGNFVAKKTSDY
                     CLVWTNAGERAATLHYEIQRASQ"
     gene            15289..16524
                     /locus_tag="VI319_15865"
     CDS             15289..16524
                     /locus_tag="VI319_15865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6865369.1"
                     /translation="MNPAAPALTPLQLAVTVAMTVVVQSLTAMALAVPPVLAPVAAAD
                     LGVDPARVGLWSGMAFLAAMFAGLACGTLVGRYGPVRMFQAAAVCVAAGLAAGAAGSL
                     ALVLSCAVLFGAAHGFVNPASSTILAAAAPARMRTMIFSIKQTGVPLGAAAAGVLLPL
                     LLLRTDWRHALAVLALASACVAPLVAPFRARYDGVRDEKTRRAHPLRLRSIVAPIAEV
                     WGDRPVLALAVTSGVYSCVQTSFLTYLISYLKLGLGYSLVTAGSVFAVSQLTGVAGRV
                     AWGALADRWGAPRLLLALLGIAMSLCGVAAILFDPSWPVALVLAVCMLYGATAVGWNG
                     VYLAEVARLAPEGRIATVTGGTQFFTFSGGLIGPPVFGTIVASTGSYGYGFVLIIILP
                     LVNAAWLLLPETRTARRGT"
     gene            16514..17158
                     /locus_tag="VI319_15870"
     CDS             16514..17158
                     /locus_tag="VI319_15870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6865370.1"
                     /translation="MEHEVEAACAPEPVVLLHGLWMNRFAMHYLVHALGRAGFRASAM
                     GYRSGSETLEQHVTRLARRVAATGGERVHLAGHSLGGLVILRYLQRAPDPRVRRAVLL
                     GAPVSGCLMAERLGKKAAFLLGSSASAWRPPFDTSLDARFEVGVVAGTRPFGLARLLV
                     RLPHPSDGVVCLDETRFPGMRDHVTLPVSHSAMLISAQVARQAAAFLREGRFAR"
     gene            17155..18219
                     /locus_tag="VI319_15875"
     CDS             17155..18219
                     /locus_tag="VI319_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY6865371.1"
                     /translation="MRRLAVLAALALVLVVSGCGTLAYYAQAVGGHLELMRLSVPIDE
                     AVHEPKTPEALRERLEQVVAIRDFASRELKLPDNGSFRSYADIGRPYVIWNVFATPEF
                     SVKPVESCFPFAGCVAYRGYYSEEAARRYADDMAAQGYDVYSGGVPAYSTLGYFNDPV
                     LSTFIRYPDAELARLIFHELAHQVVYVRNDTMFNESFAVSVEEEGVRRWLAAHGTPAQ
                     RAAYEAAAERRRQVFALVLKYRNRLDAFYRGSGTVEEKRAGKARLFREMRDDYDALKK
                     SWGGFAGYDRLFAQGPNNALLASIAAYTELVPAFHAMIVADGGDMERFYADVRRIADL
                     PPEERRARLGGPPIAAEAAR"
     gene            complement(18221..18841)
                     /locus_tag="VI319_15880"
     CDS             complement(18221..18841)
                     /locus_tag="VI319_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY6865372.1"
                     /translation="MLKLWGRVNSINVQKVLWALDELRVAYERVDAGLQFGVVNEPFY
                     RRMNPNGRVPTIEDDGLVLWESNAIVRYLAAKHGQGTLWPADLRTRADADRWMDWCTS
                     TLAPAMTPVFWGLVRTPPEKRDAAKIAAGVEQCVKALELFDGCLAEREYVAGKNFTMG
                     DIPVGTFVHRWYALPLERPKLPNVEAYYARLKARPAYAGHVARPLS"
     gene            complement(18844..19230)
                     /locus_tag="VI319_15885"
     CDS             complement(18844..19230)
                     /locus_tag="VI319_15885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6865373.1"
                     /translation="MLKLFNPKAGPVPNGPYSHGVLVPANARWLYVAGQTGTRKDGSI
                     PQTVEEQTEVVWQNILAVLAEGGMGVEDVVKVTSFLVRKDDYAKFAPVRAKYLGAHRP
                     ASTLLVVSALARPEFLVEVEAVAAKS"
     gene            complement(19232..20848)
                     /locus_tag="VI319_15890"
     CDS             complement(19232..20848)
                     /locus_tag="VI319_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY6865374.1"
                     /translation="MLQRIPGSRNSSIDCTTPYPTRRRPTLARNVVSTSQPLAAQAGL
                     RMLLAGGNAVDAALAAAITLTVVEPISNGIGSDAFAIVWDGAKLHGLNASGCSPAAWT
                     PEYFSGKTAVPQRGWNSATVPGCVAAWRDLSQRFGKLPFERLFGPAIEYARSGYPVSP
                     FIAERWDLQCAELKVHPGFAEAFMPGGHAPRAGEVFRFPDQAATLEKIAATRGDAFYR
                     GEIAERVAAHARANGGAMTREDLAAHENEWTEPLSIDYRGLTVHEMPPNGQGIVCLIA
                     LGILSHFDVAEHPVDSADSVHLQIEAVKLAFADAYRHVADPRAMELAPQRLLDRGYLA
                     SRAKLIDRRRAQNFGHGTPPRGGTVYLTAADAGGMMISMIQSNYMGFGSGVVVPGTGV
                     SMQNRGAGFVLTPGHPNQVAPRKRPFQTIIPGFITRNGLPLMSFGLMGGSMQPQGHTQ
                     LVVRMADYGQGPQGAIDGPRFRIVQGLDVNFEPEFPKATLAELAARGHHVVELPAGYM
                     DFGCSQIAHRLEDGYLAASDARRDSLAVGF"
     gene            complement(20853..22142)
                     /locus_tag="VI319_15895"
     CDS             complement(20853..22142)
                     /locus_tag="VI319_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEY6865375.1"
                     /translation="MADRKFGIETLALHAGQIPDPATGARAVPLYQTTSYVFDSADHA
                     ASLFNLQTFGNVYTRLSNPTTAVFEERMAALEGGRAALATATGQAAEMTAILTLCEAG
                     DHIVSAATLYGGTYTLLEVNLRKLGIETTFVDPDDPENFRRALTPKTKLVYCETLGNP
                     LINIVDLEAVGRIAREAGVPLVVDNTVPSPALCRPFEHGADIVVHSATKYIGGHGTTM
                     GGVVVESGKFPWGNGKFPGMTEPSRGYHGVIFHETFGDFGYTMKARMETLRTFGPSIS
                     PFNAWMLLQGLETLPVRMEKHCANALAVAKFLQGHRGVSWVNYPGLESSKYHALAKKY
                     LPKGASGLLNFGVKGGARAGEKFIEAATFMSHLANIGDAKTLIIHPASTTHRQMSEAE
                     QAKAGVTPDMIRISVGLETLDDILWDLDNALSAAAKA"
     gene            complement(22156..22641)
                     /locus_tag="VI319_15900"
     CDS             complement(22156..22641)
                     /locus_tag="VI319_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY6865376.1"
                     /translation="MVDDIPSLRRILKTTRVIAMVGLSASWHRPSYFAAKYLLEHGYT
                     VIPVNPGQSEILGQKCYKSVREAAAKSGLAIDMVDCFRKTEDIMPIADEAIAVGAKVL
                     WQQLGVVNLEAARKAEAAGLEAVIDRCVKIEHGRLFGGLNWCGVNTRVISAKRPQWLP
                     Y"
     gene            22822..23169
                     /locus_tag="VI319_15905"
     CDS             22822..23169
                     /locus_tag="VI319_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865377.1"
                     /translation="MKRKLAMIFAGSAVLGTLAFAPAAFAQDTVIVDRPVTSGSTTVV
                     TPAPDSSAVVVEPNTVVAAAPQPQAFESQYHYGFAAARQPGSVPSPYSDRPSSGHIGD
                     EFYYKQFGGVSPP"
     gene            complement(23195..24175)
                     /locus_tag="VI319_15910"
     CDS             complement(23195..24175)
                     /locus_tag="VI319_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6865378.1"
                     /translation="MRVFCAVVDNGSFAAAAGKLRLSRAMVTKHVMNLENHLNIRLLN
                     RTTRRLSLTESGAGYYERCTQILNELEEAEAQARQASAEPRGTLRINAPYSFATAHVA
                     PFLPEFLASHPDLKLDLTMNDRFVDLVEEGFDVAVRIAATLPESSLVARKLAPCRMVV
                     CGSPEYFQRHGTPRSPADLERHNCLGYSFCASQGAWTFGSADGRRHAVKSNGSIRANS
                     GDALRAAALGGIGVVLLPTFAVGRELEEGRLEAVLADCETPESAIYAVYPSRRHLSAK
                     VRAFVDFLGHKYGPDPYWDQWRRSSDGNKKTAPHEGEPSRAPESAPAGLG"
     gene            24174..24329
                     /locus_tag="VI319_15915"
     CDS             24174..24329
                     /locus_tag="VI319_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865379.1"
                     /translation="MLVNESMLAVPGQSLEFSGNRKAWAERISPELPPAERLTPVGKS
                     EAAQVQQ"
     gene            24411..>25075
                     /locus_tag="VI319_15920"
     CDS             24411..>25075
                     /locus_tag="VI319_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY6865380.1"
                     /translation="MRSGERTSAQILVDALRIHGTSTAFCVPGESYLAVLDALYEARD
                     AIRLIVCRQDGGAAYMAEAYGKVTGQPGICFVTRGPGATNASIGVHTAYQDSTPMILF
                     VGQVPGEFAEREAFQEMDYRRMFGPMTKWVAQVDRPERMHEYVSHAFHLAMSGRPGPV
                     VLALPEDMLTERGAPADVARYQVVQASPAAADVERVRAMLGRAERPLAILGGSIWGEQ
                     ARR"
BASE COUNT         4029 a         8533 c         8731 g         3782 t
ORIGIN      
        1 gccgcacgct gcgggcgatc cagatcgacg ccgccgcggc gcgaaacgat tacgccaagg
       61 cgctgtcggc ctggcgggct gggatcggct cggaggagcc gaggtagcag ggcccgcaga
      121 ggcccggaga gggaacgaca acatgcgcgc actatcgatg tcgatgctgc tcgccgccgc
      181 gacgctggtc gcgggatgcg gcgaccacga ccagaagacc gaggcaccgc cgccgcggct
      241 ggaggacggc cgcgtggtgt tctcggaagg caatccgcag ctcgcggcgc tgcagtcgga
      301 cgtcgtcaag cccggcgcgc ccgagcagct caagctgacc ggccgcctcg tctgggacga
      361 ggagcgcacc gtgcgcctgt ttccgccgtt cgcgggccgc gtcgcgcgca tcctggtcaa
      421 gcccggcgac gtcgtgaagg cgggcgagcc gctcgcgctg ctgttctcgc ccgacttcgg
      481 acaggcgcag gccgaggcgc gcaaggcgca gaccgacttc gcgctcgcga agaagagcct
      541 cgagcgtctg cgcgagctga acaccgcggg cgtcgcggcg cgcaaggatg tgaacgccgc
      601 cgaggccgac tacgcgcgag ccgaggccga gctcgcccgc gccgagggcc gcgtcaagct
      661 ctacggcgcc tcgggcgaca cggtcgacca gagcttcgcg ctgcgcagcc cgatctcggg
      721 caccgtggtc gagcgcaaca tcaacccggg acaggagctg cgccccgacg tcgccgccgg
      781 ggcgccggcg atgttcgtcg tcacggatcc ttcgcgcctg tgggtgcagc tcgacgccaa
      841 cgagcgcgac atctcgcggc tcaagagggg cctgcacgcg acgctgcgca ccggcgcctg
      901 gcccgaggag acgttccccg cgaccgtggt cgcggtcgcg gacttcatcg acccggcgac
      961 gcgcacggtg aagctgcgcg gcatcgtcga caaccgcgcg cgcaagctga aaggcgagat
     1021 gttcgtcagc gccgagatgg aaggcgcgcc gcgcgcgcag gtccaggtct cggagaaggc
     1081 catcgtccag gcgaacgggc gcaactacgt cttcatcgag gaagccccgg gccgcttcgc
     1141 gcgcgtccag gtcgatgtcg agggcgtgcg cgacggcacc gcgggcgtgg tctcgggcct
     1201 cacccagggc cagaaagtcg tcgtcgaggg caaccttttc ctgaaccgcc tgtaccgcca
     1261 gctgtccggg gacgccgcgg cctgaaatga ctgaatgaag cgcatcgtct cgttcgcgct
     1321 gcacaagccg ctgttcatcg tcctcgggct gattctgttc atcggctcgg ggctggcggc
     1381 gttcagatcg ctccccatcg aggcgtttcc ggacgtcacc gacacgcagg tgaacgtcat
     1441 cgcgctctac cccggccgcg ccgcggagga ggtcgagaag caggtgacgg tcccgctgga
     1501 ggtggcgctc gcgggtctgc cgaacgcggt gcgcatgttc tcgcacacgc agttcggcct
     1561 gtccttcatc atcatcacct tcgacgaccg agccaatccc tatttcgcgc gccagcaggt
     1621 cgtcgagcgg ctggcggccg tcgacatgcc gcccggcgtg cagccgcagc tcgcgccgct
     1681 gtcgacgccg atcggcgaga tctaccgcta ccggctgtcc tcggactcgc acatggaccc
     1741 gcgcgacctg cgcacgctcg aggactggac cgtcgagcgc cagctccgcc tgatccccgg
     1801 ggtggccgac atcgtgacca tcggcgggct gatcaagcag tacgaggtga atcccgacct
     1861 cgcgcgcctg cgcgactaca agatcacgct gcagcagctg ttcgccgcgc tcgatcgcgg
     1921 caacgccaac gtcggcggca gctacctcga gcagggacgc cagcagtacc tgatccgcgg
     1981 catcgggctg ctgcgctctg ccgacgatat cggcaacatc gtggtggccg agcggaacgg
     2041 cgtgccgatc ttgatcaaga acctcgcgga cgtctcgatc ggcgcggtgc cgcggcaggg
     2101 cgtcgtcggc caggaccgcg acgacgacat cgtcaccggc gtggtcctga tgcgcaaggg
     2161 cgagaacccc tcggaggtgc tgaaagcggt caaggccaag gtcgggcagc tcaacgcctc
     2221 gttgctgccg ccgggggtca agatcgtgcc gttctacgac cgcacctggc tgatcgagca
     2281 cacgctgaag acggtcttca gcaacctgac cgagggcgcg gcgctggtga ccctggtgct
     2341 gctgctcttc ctcggcaacc tgcgcgcggc cctgatcgtc gcgctggtca tcccgctgtc
     2401 gctgctcgcg accttcctcg gcctcacctt cgtcggcatc ccggcgaacc tgttgtcact
     2461 gggcgccatg gacttcggca tcatcgtcga cggcgcggtg atcgtggtcg agaacgtgtt
     2521 ccgcaagctc tcggatcccg ggcacgagcg cacgccgcgc ctgcagcgga tcctggacgc
     2581 gacgatcgag gtcgggcgtc cgacgctgtt ctcgatgctg atcatcatcg ccgcgcacat
     2641 cccgatcttc acgctgcagc ggcacgaggg gcgcatcttc gcgcccatgg cgtggtcggt
     2701 cacgtcggcg ctcgtcggct cgctgatcct ttcgctgacg ctggtgcccc tgctgtgcct
     2761 gcattttctg cccaagcacc tgcccgagga ggagaaccgc ctggtgcgcg cgtgcaagcg
     2821 cttatacgag ccggcgctgg tctgggcggt gggccgcaag aagatcgtga tcgggatcgc
     2881 gctcgcggcg ctcgcggcga gcttcgccgt cgtgcccaag ctcgggaccg agttcctgcc
     2941 cgagctgaac gagggctcga tctggatcaa cgtcacgctg ccttcgtcgg tgtcggtgac
     3001 cgaggcgcag gcgatgaccg ggaagttccg cgcggtgctc gccaagatcc cggaggtcga
     3061 caccgtcatc tcgaagtccg gtcgtcccga ggacggcacc gacccgaagc tgatcaacat
     3121 gtgcgagatg ctcgtcgtgc ccaagcccga gtcggaatgg cgtccgggga tgaccaagcg
     3181 caagctggtc gaccagatgg acgacgccat gtcggtgttc cccgggatcg agccgagctt
     3241 ctcgcagccg atccgcgaca acgtcctcga gtcgatctcg cagatcgacg gccagatcgt
     3301 catcaaggtg ttcggcgacg atctcgacgt cctgagcgag caggctcagg ccgtgctgca
     3361 tgcgatctcc gacgtgcgcg gcgtgaagcg cgcgttcatc gaccggctgg gcgagctgcc
     3421 gcagctgctc atccgcatcg accgcgaggc tgccgcgcgc tacgggctga acgtcgccga
     3481 catccaggac gtgatcgaga tggtcctcgg cggcaagccg gcgacgcagc tctgggaggg
     3541 cgagcggcat ttcgccgtca ccgtgcggct gaaggacgcg cagcgcacga tccccaacct
     3601 cgaggcgatc accgtcgcca cgcccggcgg cgcctacgtg cccctgtcgc aagtggccga
     3661 gttcaagacc gtcggcggcg ccatgaacat cgcgcgcgag gacggcgtgc gcgtctacgc
     3721 gatcggggtg ttcatcgccg accgcgacat gggcagcgtg gtggccgaca tgaaggaccg
     3781 cgtcgccgcg caggtcaagc tcaagccggg gtaccgcgtc acctggtcgg gcgagttcga
     3841 gaaccaggag cgcgccatgg cgcggctggc gatcatcatc ccggtttcgg tgctcctgat
     3901 cttcctcctg ctcttcgacg ccttcggacg ggtctcgagc gcgacgctca tcctgctcaa
     3961 catcccgttc gcgctgatcg ggggcatctt cgcgctcctc gtgaccggga tcccgctgtc
     4021 ggtgtcggcg gcgatcggct tcatcgcgct gttcgggcag gcggtgctga acggggtggt
     4081 gatggtcgcc tatttcaacc agctgcgggc gagcgggatg cccccggagc aggcggtgat
     4141 caagggctcg ctcgtgcgca tgcggaccgt gctcatgacg ggcctgctcg cgatgctcgg
     4201 cctcctgccc atggcgattt cgaccgggat cggctcggag acccagaagc cgctcgccgt
     4261 ggtggtcatc ggcggcctgg tgtcggcgac gctgctgacc ctgatcgtgc tgcctgtcct
     4321 gtacctcgtc ttccagcgcc accaccacgc gcgccccgcg gcggggggcc agccgcattg
     4381 aaccgccggg aggcggccca ggggcccggc cggaccctgt gggaaacccg caaaaactgc
     4441 tagaatccgg ccctttccga aagcaccgag gaccgccgct agatgccgac cgtacgcgtc
     4501 aaggaaaacg agccgttcga ggtcgcgctt cgccgattca agcggaccgt cgagaagacc
     4561 gggctgctga ccgagctgcg cgcccgcgag ttctacgaga agccgacggc gtaccgcaag
     4621 cgcaagctcg cggccgcgat caagcgccac tacaagcgtc tgcgcaacca gacgctgccg
     4681 cccaagctgt actgagccgc gcgcgcgaat gcgctggatc ccgggccgga caggggaacc
     4741 tgtccggcct cgtcgtttct gagcccgacc cgacggagct tcccatgccg ctgaaagaac
     4801 gcatccagga agacatgaaa gccgcgatga aggcgcgcga gacgcagcgc ctgtcggcga
     4861 tccggctgct gctcgcggcc atcaagcagc gtgaggtgga cgagcgcaag gacctctccg
     4921 accccgagat cgcctccgtc atcgagaaaa tgatcaagca gcggcgcgac tccattgctc
     4981 aatacacgag cgccaatcgg accgacctcg ccgacgccga gaccttcgag ttgaacctgc
     5041 tcgccgggta tctgccgaaa cagctgtcgg acgcggaaat cgcgaaggcg gtcgacgacg
     5101 ccctcgcgca gaccggggcc aaaggggcgg ccgacatggg caaggtcatg ggcgcgctga
     5161 aggcgaagct ggccggccgc gccgacatgg cgaaggtgtc cgccctggtg aaggcgaagc
     5221 tcgcgggttg atctcgcggg gccggggatt tgactggccg gcgttttggc ctatatatgt
     5281 agcaggcccg tgattccaga ttcgttcaag caagaattgc tccatcgcgt cgacatcgtc
     5341 gacgtgatcg agcgtcacat gacgctcaag aaggccggcg cgaacttcgc cgcctgctgc
     5401 cccttccaca gcgagaagac accctcgttc acggtaagcc cgaccaagca gttctaccac
     5461 tgcttcggct gcggcgcgca cggcaacgcg atctcgttcc tcatggagta ctccgggctc
     5521 gggtacgtcg acgcggtgcg cgagctggcc gaggacgtcg gcatgaagat gcccgagttc
     5581 gagccgcgcg cgaagaagga ggagaccggg cccgacctgc atccggtcat gcagcgcgcc
     5641 tgcgactact atcgcgagca gctgaaggct tcgccgcgcg cgatcgatta cctgaagcac
     5701 cgcgggctga ccggcaaggt cgccgcgcgc ttcggcatcg gctatgcgcc gccgggctgg
     5761 caaaacctcc agagcgtatt tcccgggtac gcggacaagg cgctcaagga cgcgggactc
     5821 gtgatcgacg gcgaggccgg gcgccgctac gaccgcttcc gcgaccgcat catgttcccg
     5881 attcaggatc agcgcggctc ggtcatcggt ttcggcggtc gcgtgatcga gccgccgctc
     5941 gacgatgagc gggaccccgg cccgaagtac ctcaactctc cggagacccc gctgttccag
     6001 aaggggcagg aactgtacgg cctgccgcag gcgcggcaag cgatccgggc ggcggggcgg
     6061 gtgctggtcg tcgaaggcta catggacgtc gtcgcgctcg cccagcacgg cgtcgagtac
     6121 gcggtggcga cgctcggcac cgcgacttcg gcgacgcacg tgcagcggct gctgcgccag
     6181 accgacgaga tcgtgttctc gttcgacggc gacgccgccg gccgcaacgc ggcctggaag
     6241 gccctcgagg tgagcctgcc cgcggtgacc gacacgaagg tggtgagatt tctttttctg
     6301 ccgcccgagc acgatcccga tagttttgtg cgggaacggg gggtccaggc attcgaggaa
     6361 gccgtgggga aagcggagcc gttggctcaa ttcttcgccg agggattgaa ggagcgcctg
     6421 gggaccgggg ccgtcatgga agggaagtcg catttcttcc atcaggcgaa ggcttttctc
     6481 cagcctctgg ccgccatcaa cctgaaacaa aacctgctca agcatgtggc gcagatctac
     6541 cggatttccg aggaagaggc tgccagggac ctcggtatct cgattgccag gacacccgct
     6601 tcgcgttacg ggcacgtccc cgcccgcgag gagcgcgcgc cgatgcagag cctcgaggtg
     6661 tggctgctga agcgcctgat ggtgaagcct tcgctcgccg cggagatgcc cgcggagctg
     6721 ctgcggggac agagcttcga gtcgctcgcc cttcaggtct tcgtcgcgtt cgcgcgcgag
     6781 accgcaggcg cgaacttcga cgcgctggtg atcgatcgtt tccacgggac cgagttcgag
     6841 ggactgttca agcagatcaa aaaggaagtc atggtgatgg cgctgtcgcc cgaggacgtg
     6901 gagatggaat tccgcgacgc gctccccaat atggagctac agcacgtcaa ggaccggctc
     6961 gccgagctgg tgcgccaggc cgagctgcgc aggctcacgc ccgaggagga ggcgcagctc
     7021 gcgaggctcg cggcgaggcg caaggagctc gacgtcgtcc gcagcgggtc cccgcccgtg
     7081 ctataattat cggttcactt ttaatcgcga ccttcaggaa actttatgtc gaaacagagg
     7141 cttaagtcca aggcgaaagc ccggcccgct ggccgggcca agggcaagat cggcctgaag
     7201 agcccgcgcg cccgcgagat ggacaagctg aaggcgcaac gcgagaaggc ggccgcgaag
     7261 ctgcgcgcgc agaaggagcg cgagcgcgac aggatgcagc gcgagcgcga cagggaacgt
     7321 gcacgcaagg agaaggaagc ggcgcacgcg aaggccgcga aagaaaggga gcgcgcgcgc
     7381 cgcgagaaag aagcgcagaa ggcgcgcgag cgcacggaaa aggagcgcga gcgcctgcgc
     7441 cttcagaagg agaaattgcg cgagcgcgag cgcgtcgcga aagaaaaggc gaaggcaaag
     7501 gaactagccg cgcgggaaaa ggccaaagcc aaggaactcg ctgcgcaaga aaaagcgaag
     7561 gccaaagaag ctgcggcgtt gcagaagcag aaggaaagag agttgaaggc gcagttgaag
     7621 atgcaagcgc aagagctcat gaagttgcgt aaggctggcc gcggctcccg tcgcaacggc
     7681 gatgccgagc gtgcgctgct tctccgcgcc gaggcgcgcc ccgaagacgc agaggcgcgg
     7741 cgcacgcgcc tgaagaacct gatcaagctc ggcaaggagc gcggcttcct cacttacgcc
     7801 gagatcaacg atcacctgcc cgacgacatg gtcgacgccg agcagatcga gtcgatcatc
     7861 acgacgttca acgacatggg catccaggtg tacgacgagg ccccggatgc cgagcagttg
     7921 ctgatctccg agaacgcgcc gccggtcgtc cccgacgagg acgccgagga ggaggccgag
     7981 gccgcgctgt cgacggtcga ccccgagttc ggccgcacca cggacccggt gcgcatgtac
     8041 atgcgcgaaa tggggtcggt ggagctgctc acgcgcgaag gcgagatcga gatcgccaag
     8101 cgcatcgagg acggcctgaa gcacatgatc caggccatct ccgcgtgccc gacgacgatc
     8161 gccgagatcc tggagctctc ggcgaaggtc gcgcgcgacg agatgcgcat cgacgagctg
     8221 gtcgacggcc tgatcgatcc gaacgcgaag gaagagccga tcaccgagct cgccgacgac
     8281 gtcgagcccg aggaggaaga ggagggagag ggcgaggagg acagcgccgc gctctccgcg
     8341 agcctgctcc agctcaaaga ggacgcgctc gcccgcttcg ccaagatcca gcgcctctac
     8401 gagaagatga agaaggcgct cgtcgagcac ggccaccgca gcaaggaata catccgcgta
     8461 cgcgaccaga tcggcggcga gctgacgaac atccgcttca ccgcgcgcat catcgagcgt
     8521 ttgtgcgaca gcctgcgcgg catggtcgac gaggtgcgcg gccacgagcg cgagatccag
     8581 gcgctgtgcg tgatgaagag caacatgccg cgaccgcatt tcatcaaggt cttccccggc
     8641 aacgagacca acatgcgctg ggtgaagaac gagatcgccg cgacccggcc gtggagcagc
     8701 gtgctgacgc gctacgagcc ggccatcctc gagcagcagc agaagctgct cgcgatccag
     8761 aagcgcatcg gcatcccgct caaggatctc aaggagatca acaagcagat gtcgaccggc
     8821 gaggcgaagg cccgccgcgc gaagcgcgag atgaccgagg cgaacctgcg cctggtgatc
     8881 tcgatcgcga agaagtacac caaccgcggc ctgcagttcc tcgacctgat ccaggaaggc
     8941 aacatcggcc tgatgaaggc ggtcgacaag ttcgaatacc gccgcggcta caagttctcg
     9001 acctatgcga cgtggtggat ccggcaggcc atcacgcgct cgatcgccga ccaggcgcgc
     9061 accatccgca tcccggtgca catgatcgag acgatcaaca agatgaaccg catctcgcgc
     9121 cagatcctgc aggagacggg ccaggagccc gacccggcga cgctcgcgca gaagatggag
     9181 atgcccgagg agaagattcg caagatcctc aagatctcga aagagccgat ctcgatggag
     9241 acgccgatcg gcgacgacga cgactcgcac ctgggcgact tcatcgagga ccaggcgacg
     9301 ctcgcgccga acgacgcggc gatgtacgcg agcctgcgcg aggtcaccaa ggacatcctc
     9361 gactcgctca ccccgcgcga ggccaaggtg ctgcgcatgc gcttcggcat cgaaatgaac
     9421 accgaccaca cgctggaaga ggtcggcaag cagttcgacg tcacgcgcga gcgtattcgc
     9481 cagatcgagg ccaaggcgct gaggaagctg cggcatccgt cgcggtcgga gcggctgaag
     9541 agcttcctgg acaatagcta gagccgggcc tcgtgcgccg gctggaacga aaacgccccg
     9601 cgatgcgggg cgttttcttt gcggctgtag aattgactga agggcctgta gctcagtcgg
     9661 tcagagcaga ggactcataa tcctttggtc cctggttcga atccaggcgg gcccaccaac
     9721 acgcccagca gacgctcgcg gaaggcgccg gccatgtcgg catcgggtcc gaggagctcg
     9781 acgagctcgt ccagccgcat cacgcaggcg tcgatctccg aggccagatt ggccgcgagc
     9841 gcggcgcggc ggcgctgctc gaccaggccc ccgagctgct cgtcgcagcg cgcgatcggg
     9901 ccggggtatg cgcgcacctc tgcgttcagc gcgccgctcc agtcatcgag ctcctgcctg
     9961 aactgccgcc aagcttccgc tatggatttc atcgccgccc ccggagcatt ccgatggcgg
    10021 cattctgcca cgtccggcgc cgtgctactt catccgcgcg agcaccatca ccgatccgat
    10081 ggcgaccgcg aacgcacccg cagcgatgac ggcgcccgcc agccgcgggt agtgctcgac
    10141 ccacttgtcc agcggcatgt acatcgcgtc ggcgacgccg ccgaacgcct tgcgcgacga
    10201 gtgccattcg ttcgcgcgcg cctcgagctt gcgcagcgtc tccggcatga ggacgagcag
    10261 cgcgccgacc gccgccgcgg cgatgctgcc gaccacgagg aaccagcgca ggctgtcgag
    10321 cagccagtgc gcggccggcg tccagccgcc caggctgttg gcgggcaaga ccgcgtgcac
    10381 gttgaatcgc gtcaggaggc cgaacagcga atacgcggcg gccaggacga agaacaggcc
    10441 gatccagagc cggtaacgct ggacgcccga gctcgtgtcg tgcgggatct ctgcagactt
    10501 gagccagcgc cgcgtcgaca cccaccggtt cagaaccgcg aggaagcgca gtgtcggctc
    10561 cctccataga atcaggccga ttccgaccag gacaccgacc acgccgacca gcaggaaaaa
    10621 ctcgacgaca gacgggaaaa gcagcgtctg catgaactcg ttcttgaaca tatctccctc
    10681 gccttctcgg aagcgtccgc gcaggctgcc gcagtttccg ccgcttggca agggatttac
    10741 gtagaataag ccggactttc tggaggagga tccatgagag cacgaaacgc tgccgtcttc
    10801 ctgtgcgcgg cgctggcatg gggcacggcc ctcggccagg cgtggccgag caagcccatc
    10861 cattacctcg ttccgttccc gccagcgggc gcaaccgaca tcaccgcgcg catcatcgcc
    10921 gacaagatca cgggcccgct cgggcagccg gtcatcgtcg agaaccggcc cggcgcggcg
    10981 ggcaacgtcg gcagcgaggt ggttgccaag tcgacgcccg acggctacac cgtcctgcag
    11041 tgcaccgtgg cgcagtcgat cagccagacg ctgtacgcga agctgaacta cgacctggag
    11101 aaggacctcg cgccggtcgc gatgatcgcg ctcgttccga acgtcatgga ggtcaatccc
    11161 tcggtgcccg cgaaatcggt cgccgagttc atcgcgctcg cgaagagcaa gcccggcaag
    11221 atcaatttcg cgtcctcggg cagcggcacg tcgatccaca tgtcggccga gatgttcatg
    11281 atgctgaccg gcgtgaagat ggtccacgtg ccctacaagg gcagcggccc tgcgctcgcc
    11341 ggcatgctcg gcggcgaggt cgacgtcatg ttcgacaacc tgacctcgtc gatcgcctac
    11401 atcaagagcg gaaagctcag ggcgctcgcg gtgaccacgg cgacgcgcta ccccgagctg
    11461 cccgacgtgc cgaccatgca ggaggccggc gtcgcgggct acgaggcgac ggcctggttc
    11521 gggatgctgg cgccgaaggg caccccgcgc agcgtcgtcg cgcgcctgaa cgccgaagtg
    11581 aacaaggcgc tcgcgcaacc cgacgtgaag gagaagctcg cccagcaggg cgcgatggcg
    11641 agcccgtgga cccccgagca gttcggcgag ttcatccaca acgagatcgc caagtggggc
    11701 aaggtggtga aggcctcggg cgcgaaggtg gagtgacgcg ggcctaggac gcgaggaagc
    11761 gcccggtaag ttcggcgaag cgctcgggcg cgtcccactg caccaggtgc ttgcagcgct
    11821 cgacgaggtg caggtcgagc cccgggaagc gctccagcgc gagccgcgtc cgccgcgcgc
    11881 agtcgccgcg gtcgtccgcg ccgtagatca ggcgcagcgg gacccgcacc tcgcccagcc
    11941 gctgccatag cggcgcggcg tccttcttct cgccggcccc gcgcgcctgg cggcgggcga
    12001 ggaaggcgcg gaagttcctc cccgtgctca tgcggtgacg aagcgcgacc gcctccggcg
    12061 tgaccagctc gcgatggaac accgtcgctt cgagcgccgc gcgcgtgtcc tcgagcgtcg
    12121 gctcggcggc ctcgccctcg tcaccttcgc ctggggcgcc tgctccggga agcggcggca
    12181 ggaggctgcc ggtcgcgacc accaccgcgc gcgcgacgcg cccggggtgc gacagcgcga
    12241 ggctcaccgc gaaacgtccc gactgcgagt gtccgaccag gtgcgcccgc tcgagcccga
    12301 gcgcgtccat gaaggccggc acgaaacgca cgcgaaagcc caccgagtga tcgtcggcgt
    12361 cgtcgctcag gccgaacccg ggctggtcga acgcgatgac gcgcaggccg tgcgcggcga
    12421 gcgcgtccag gttgcccgtc cagacgtcgg ccgaggagcc gagcgacgcg ccgtgcagga
    12481 gcagcgccgc gggtcccgcg ccgcgttcga tgtatcgcgt gcgcagcccg ccgacgtcga
    12541 cggtgcggtc cgcgggcgcg ccggcgctca tcgctcggcc acgtcgatca ggacgccgtc
    12601 cgggtcggcg aggtggtagc gcccgtgcgg gcacgaggcg aagagcttga ggcgcgcctc
    12661 gccctcggac ccggcgccca ccggcagcgg gcgcagatgc gggttgttcg cgccgacctt
    12721 ggcaatgtgc tgctcgagcg cggcgatgct ctcgaccttg aacccgatgt ggtcgggccc
    12781 cgggcgcgcg atgccgccgc cgtgatagtc gaggatgctc cagggcatga gaaccagggc
    12841 gacgcggcca tcgctgagcg cgaggctgcc gtcgggggct ttcctcgcgg aaggctcgag
    12901 gccgaacacg tcggtataga actcggcggt gcgctcggga tgcagcgtgc ggatcgcgat
    12961 atggctgatc gcgcgcgcct gctcgcgctc ggcctcgacg tagaccgagg cccggttctc
    13021 catgctccgc tgcgagagat cgaatatgtt gccgtcgggg tcgtgcgtgc tgacgccagc
    13081 gaacggacgc gtgctcggcc gcgccagcac ctggatcgcc gggtagcgct cgcgcaggcg
    13141 cgcgcgcacc gcgtcggcgt cgtcgacctc gaggccgaag tggtcgaggc ccgcgccgcg
    13201 gcctgccttg cgcggattga tgttcagccc gacgtagccg tcgcttacgg tgaccgcgcg
    13261 ctcgggccgc gtgtccgatg ccgggcgcat gccgaagatc gcctcgtaga agcgcccctc
    13321 gagcgcgtac tgctcgctca cgatggcgat gtgccggatc cttgcggaca tgatcagcgg
    13381 gccttcagga agatgtcgcg gaattcgcgc tcgctgcggt cccgctcgcg cgcgagcttc
    13441 gggtcgacgt agtggatcct gagccggctc tcgtcgggcc ccgcgccggg aaggtcggtg
    13501 cgcgccggca cccggccgcg cgagcggatg atggtctggc cctcgaccga aagcagcagg
    13561 tcgaccagca ggcgcgccgc gttcggatgc ggtgcgccgg cggcgaccgc gacctggctc
    13621 ggcgacgtga tgatcgggtc gaggctgcgc acccagtcca ccggcgcgcc ggtcttcttc
    13681 tccccctcga cctcggccgc gtgcacgagc gcgagcggga agtcgcccgc gacgagcagc
    13741 ttcgacagca ggctgtggcc gcgccgcagc tgcggctgct ggcgcgcgag cgcctgcatg
    13801 aacgcggttc cgcgctcgcg cccgagatag tcgagcatcg cggcgtacca ctcgctgtcg
    13861 tcctcgtcca tcgcgaactt gcccttccag cgcggctcga gcaggtcctg ccagctccgg
    13921 ggcgcttgcc ccggcggcac ggcgcgcagg ttgtacccga tgacgaactg ccggacgtag
    13981 atcgccgccc agcggccgcg cggatcgacg gcgccgggcg ggaagcccga gcgcgtctcc
    14041 ggggacgcgt acgcggcgag gagctcttcc cggtcgagcg cgtccatgtc gagatgattg
    14101 aacgagacga cgtcccagag gtgcttgccg gcgcgcgcct cggtcaggat gcgcgtgcgg
    14161 atcttctcgc cgggctggcg caggatcgtg agctgcagga acggatagcg cgcctgcagc
    14221 gcgtcgcgca acggctcggc gtcggtcgtc gtcatggcgg tataccagac gaccgcgcgc
    14281 tcattgcggg cggcgtcgat gctctgcgcc gcggcgggcg ccagcacgag caggccggcc
    14341 gcggcgagac tcgcgagaag ggtgcggcga ttcggcgtca tgcgtgtccc gtgcacacga
    14401 taggtcactc gccgcgcgcg cgtcaacgcc ctcagcccgc gaagcgcgcg cctggtgtaa
    14461 aattcgttcg tgccacgaag cgcactgagc ggactcgggc tgctgctggc gatcctcatc
    14521 gccccgcccg tcgtcgccca ggcgcccgtg gaggagcggc aacgccgcac cgattcctat
    14581 accgaggcgc agcagcgcgt cgagttctcg cggcagggcg ccgagcgcgc cgcgcgccgc
    14641 gtgaaggacg ccgagcaggg cctgaaggat gccgaggccg agctcggtcc ggcccagcga
    14701 cggtacgagg acagcaaatc ccgcgtggag agggcgcgca ccgagctgaa gcaggcgcgc
    14761 gcggtggaga gcgagtcgcg caagacctac gaggcggaat cggccgcctt ccagcggttg
    14821 cgcgcgcgcg gcaacgacac gaaggggacg aaatgaacct caggctgatg gcggctgtgc
    14881 ccgggctcgc cgtcgcgctg gcagtgccgt gcgcgcgtgc gcagcaggac ggtcagcccg
    14941 aggcgcccaa ggtggtgacg aaaacgctgg tgaagtcgga ggcccagaag gaatgcttca
    15001 agctggtcaa cgcgcaggtg ctgtattacc ggttcaacgc ggacggtccc ctggatttca
    15061 agctctcgca ccaggaggac aaggagatcg tccatatcgc gcgcaccggc atcgaggacg
    15121 cggccggcaa tttcgtcgcc aagaagacct ccgattactg cctcgtctgg accaacgcgg
    15181 gcgagcgcgc cgccaccctg cactacgaga tccagcgcgc gtcgcagtag caccgcgccc
    15241 gggccgcgcc gcgcgtccat ttccggcggg ccttctcgag ccgaccgcgt gaacccggcc
    15301 gcgcccgctc tcactccttt gcagctcgcc gtcaccgtcg ccatgacggt ggtggttcag
    15361 tcgctgacgg cgatggcgct cgcggtgccg ccggtgctcg cgccggtcgc tgcggccgac
    15421 ctcggcgtcg atccggcgcg cgtcgggctg tggtccggga tggcgtttct cgcggcgatg
    15481 ttcgccggcc tcgcctgcgg cacgctggtc gggcgctacg gcccggtgcg catgttccag
    15541 gccgccgcgg tgtgcgtggc cgccggcctc gcggccgggg cggcgggaag cctggcgctg
    15601 gtcctgtcgt gcgccgtgct tttcggcgcc gcgcacgggt tcgtcaatcc cgcgagctcg
    15661 accatactcg ccgcagccgc gcccgcgcgc atgcgcacga tgatcttctc gatcaagcag
    15721 accggcgttc cgctcggcgc cgcggccgcg ggcgtgctgc tgccgctgct gctgctccgg
    15781 accgactggc gtcacgcgct cgccgtgctc gcgctcgcga gcgcgtgcgt cgcgcccctc
    15841 gtcgcgcctt tccgcgcgcg ctacgacggc gtccgcgacg aaaagacgcg gcgcgcgcac
    15901 ccgctgcgcc tgcgcagcat cgtggcgccg atcgccgagg tctggggcga tcgcccggtg
    15961 ctcgcgctcg cggtcacctc gggcgtgtac tcgtgcgtgc agacgagctt tctgacctac
    16021 ctgatctcgt acctgaagct cggcctcggc tactcgctcg tgaccgcggg ctcggtgttc
    16081 gcagtctcgc agctcacggg cgtcgccggc cgggtcgcct ggggcgcgct cgccgaccgc
    16141 tggggtgcgc cgcggctgct gctggcgctg ctcggcatcg cgatgtcgct gtgcggcgtc
    16201 gccgccatcc tgttcgatcc aagctggccc gtcgcgctcg tgcttgcggt gtgcatgctc
    16261 tacggcgcga ccgcggtcgg ctggaacggc gtgtacctcg cggaggtcgc gcgccttgcg
    16321 cccgagggca ggatcgcgac cgtgaccggc ggcacccagt tcttcacctt ctcgggcggg
    16381 ttgatcgggc cgccggtctt cggaacgatc gtcgcctcga ccggcagcta cggctacggc
    16441 ttcgtgctga tcatcatcct tcccctggtg aacgcggcat ggctgctgct tcccgagacg
    16501 cggacggcgc gccgtggaac atgaagtcga ggccgcgtgc gcgccagagc cggtcgtcct
    16561 cttgcacggg ctgtggatga accgcttcgc catgcactat ctcgtgcacg cgctcggccg
    16621 cgccggcttt cgcgcctcgg cgatgggcta ccgctcggga agcgagacgc tcgagcagca
    16681 cgtgacgcgt ctcgcgcgcc gcgtcgccgc gaccggcggc gaacgggtgc atctcgccgg
    16741 gcacagcctg ggaggcctcg tgatcctgcg ctacctgcag cgcgcgcccg acccccgcgt
    16801 gcgccgcgcg gtcctgctcg gcgcccccgt gtccgggtgc ctgatggccg agcgcctggg
    16861 aaagaaggcg gcgttcctgc tcggcagcag cgcctccgcc tggcggccgc cgttcgacac
    16921 gtcgctcgac gcgcgcttcg aggtcggcgt cgtcgccggc acgcggccgt tcggcctcgc
    16981 gcggctgctc gtgcggctgc cgcacccgag cgacggcgtg gtgtgcctcg acgagacgcg
    17041 gttcccgggc atgcgcgacc acgtgacgct tcccgtcagc cacagcgcga tgctgatctc
    17101 ggcgcaggtc gcgcgccagg ccgcggcctt cctgcgcgaa ggacggttcg cgcgatgagg
    17161 cgcctcgccg tgctcgccgc gctcgcgctg gtgctcgtgg tctcgggctg cggcacgctc
    17221 gcgtactacg cccaggcggt gggcgggcat ctcgagctga tgcgcctctc ggtgccgatc
    17281 gacgaggccg tgcacgagcc gaagacgccc gaggcgctgc gcgagcgcct cgagcaggtg
    17341 gtcgcgatcc gcgacttcgc gagtcgcgag ctgaagctgc ccgacaacgg cagcttccga
    17401 agctacgccg acatcggccg gccgtacgtg atctggaacg tgttcgcgac gccggagttc
    17461 tcggtgaagc cggtcgagtc gtgcttccct ttcgcgggct gcgtcgccta ccgcggctac
    17521 tactccgagg aagcggcccg ccgctacgcc gacgacatgg ccgcgcaggg ctatgacgtc
    17581 tactcgggcg gcgtgcccgc gtactcgacg ctcggctact tcaacgatcc cgtgctctcg
    17641 accttcatcc gttatcccga cgccgagctc gcgcgcctga tcttccacga gctcgcgcac
    17701 caggtggtgt acgtgcgcaa cgacaccatg ttcaacgagt cgttcgcggt ctcggtcgaa
    17761 gaggagggcg tgcgccgctg gctggcggcg cacggcacgc ccgcgcagcg cgcggcgtac
    17821 gaggcggccg ccgagcgccg ccggcaggtg ttcgcgctcg tgctcaagta ccgcaaccgc
    17881 ctcgacgcct tctaccgcgg ctcgggcacg gtggaggaga agcgcgccgg caaggcgcgc
    17941 ctgttccggg agatgcgcga cgactacgac gcgctcaaga aatcctgggg cggcttcgcc
    18001 ggctacgacc gcctcttcgc ccagggcccg aacaacgcgc tgctcgcgtc gatcgccgcg
    18061 tacaccgagc tcgtgcccgc gttccacgcg atgatcgtcg ccgacggcgg cgacatggag
    18121 cgcttctacg ccgacgtgcg caggatcgcg gacctcccgc ccgaggagcg ccgcgcgcgg
    18181 ctcggcggcc cgccgatcgc ggccgaggcc gcgcgctgac tcagctcagc ggacgcgcga
    18241 cgtgcccggc gtatgccggg cgcgctttca gccgcgcgta gtacgcctcg acgttgggca
    18301 gcttcgggcg ctcgagcgga agcgcgtacc agcggtgcac gaacgtgccg accgggatgt
    18361 cgcccatcgt gaagttcttt ccggcgacgt actcgcgctc cgcgaggcag ccgtcgaaga
    18421 gctccagcgc cttgacgcac tgctcgacgc cggccgcgat cttcgccgcg tcgcgcttct
    18481 cgggcggcgt gcgcaccagc ccccagaaca ccggcgtcat cgcgggcgcg agcgtgctcg
    18541 tgcaccagtc catccagcgg tcggcgtcgg cgcgcgtgcg caggtcggcc ggccagagcg
    18601 tgccctgccc gtgcttcgcg gcaaggtagc gcacgatcgc gttcgactcc cagagcacga
    18661 ggccgtcgtc ctcgatcgtc ggcacgcggc cgttcgggtt catcctgcgg tagaagggct
    18721 cgttgaccac gccgaattgc agcccggcgt cgacgcgctc gtacgcgacg cgcagctcgt
    18781 cgagcgccca cagcaccttc tgcacgttga tcgagttgac gcgcccccag agcttcagca
    18841 tggtcaggat ttcgccgcga ccgcctcgac ctcgaccagg aactcgggcc gggcgagcgc
    18901 cgagaccacc agcagcgtcg acgcggggcg atgcgcgccg agatacttcg cacgcaccgg
    18961 cgcgaacttc gcgtagtcgt ccttgcgcac gaggaacgag gtcaccttga cgacgtcctc
    19021 gacgcccatg ccgccttcgg cgagcacggc gaggatgttc tgccacacga cctcggtctg
    19081 ctcctcgacg gtctgcggga tcgagccgtc cttgcgcgta ccggtctggc ccgcgacgta
    19141 gagccagcgc gcgttcgcgg ggacgagcac gccgtgcgaa tagggaccgt tggggaccgg
    19201 gcccgccttg ggattgaaga gcttcagcat gtcagaaccc caccgcgagc gagtcgcggc
    19261 gcgcgtccga ggcggcgagg tagccgtcct ccagccgatg cgcgatctgc gagcatccga
    19321 aatccatgta gcccgcgggc agctcgacca cgtggtgccc gcgcgcggcg agctcggcga
    19381 gcgtcgcctt cggaaactcg ggctcgaagt tgacgtcgag cccctggacg atccggaagc
    19441 gcgggccgtc gatggcgccc tgcggcccct ggccgtagtc ggccatgcgc accacgagct
    19501 gcgtgtggcc ctgcggctgc atgctgccgc ccatcaggcc gaagctcatg agcggcaggc
    19561 cgttcctggt gatgaatccc gggatgatgg tctggaacgg ccgcttgcgc ggcgcgacct
    19621 gattgggatg tcccggcgtc aggacgaagc cggctccgcg attttgcatg ctgacgccgg
    19681 tcccggggac gacgacgccc gacccgaagc ccatgtagtt ggactggatc atcgatatca
    19741 tcatgccgcc cgcgtcggcc gcggtgaggt agacggtgcc gccgcgaggc ggcgtgccat
    19801 gaccgaagtt ctgcgcgcgc ctgcggtcga tcagcttcgc gcgcgaggcg aggtagcccc
    19861 gatcgagcag ccgctgcggc gcgagctcca tcgcgcgcgg atcggcgacg tggcggtaag
    19921 cgtcggcgaa ggcgagcttc accgcctcga tctggaggtg gacgctgtcg gcggagtcga
    19981 ccggatgctc ggcgacgtcg aaatgcgaaa ggatcccgag cgcgatcagg cagacgatgc
    20041 cctggccgtt cggcggcatc tcgtgcacgg tgaggccgcg gtagtcgatg gagagcggct
    20101 cggtccactc gttctcgtgc gcggcgaggt cctcgcgcgt catcgcgccg ccgttcgccc
    20161 tggcgtgggc ggcgacgcgc tccgcgatct cgccgcggta gaacgcatcg cctcgcgtcg
    20221 ccgcgatctt ctccagcgtc gcggcctggt cgggaaaccg gaacacttcc cctgcgcgcg
    20281 gtgcgtgccc gccgggcatg aaggcttcgg cgaaccccgg gtgcaccttc agctccgcgc
    20341 actgcaggtc ccagcgctcc gcaatgaacg gcgagaccgg gtagcccgag cgcgcgtact
    20401 cgatcgccgg gccgaacagg cgctcgaacg gcagcttgcc gaagcgctgc gacaggtcgc
    20461 gccaggcggc gacgcagccc ggcacggtgg cgctgttcca gccgcgctgt ggcacggcgg
    20521 tcttgcccga gaagtactcg ggcgtccacg ccgcgggcga gcaccccgag gcgttcagcc
    20581 cgtgcagctt cgcgccgtcc cagacgatgg cgaaggcgtc cgagccgatg ccgttcgaga
    20641 tcggctcgac gaccgtgagc gtgatcgcgg ccgcgagcgc ggcgtcgacc gcgttgccgc
    20701 ccgcgagcag catgcgcagc cccgcctgcg cggcgagcgg ctgcgaggtg gagacgacgt
    20761 tgcgcgcgag cgtcgggcgg cggcgcgtcg ggtagggcgt ggtgcagtcg atcgagctat
    20821 tccgagatcc gggaattctc tggagcatag gttcaggcct tggccgcggc ggagagcgca
    20881 ttgtctaggt cccagaggat gtcgtccagc gtctccaggc ccaccgagat ccggatcatg
    20941 tcgggcgtca cgccggcctt cgcctgctcc gcctccgaca tctggcggtg cgtcgtggac
    21001 gcggggtgga tgatgagcgt cttcgcgtcg ccgatgttgg cgaggtggct catgaaggtc
    21061 gccgcctcga tgaatttttc tccggcccgg gcaccgccct tcacgccgaa gttcagaagc
    21121 ccgctcgcgc ccttggggag gtacttcttc gcgagcgcgt ggtacttgct gctctcgagg
    21181 ccgggatagt tcacccacga cacgccccgg tggccctgca ggaacttcgc gaccgcgagc
    21241 gcgttggcgc agtgcttctc catgcgcacg ggcagcgtct cgagcccctg cagcagcatc
    21301 caggcgttga acggggagat cgacggcccg aaggtgcgca gcgtctccat gcgcgccttc
    21361 atggtgtagc cgaagtcgcc gaaggtctcg tggaagatga cgccgtgata gccgcgcgag
    21421 ggctcggtca tgcccgggaa cttgccgttg ccccagggaa acttgcccga ctcgaccacg
    21481 acgccgccca tggtggtgcc gtgcccgccg atgtacttgg tcgccgaatg cacgacgatg
    21541 tcggcgccgt gctcgaacgg gcggcacagc gcgggcgagg gcacggtgtt gtcgacgacc
    21601 agcggcacgc ccgcctcgcg cgcgatgcgc ccgaccgcct cgaggtcgac gatgttgatg
    21661 agcggattgc cgagcgtctc gcagtagacg agcttggtct tcggcgtcag cgctcggcgg
    21721 aagttctcgg ggtcgtccgg gtccacgaac gtcgtctcga tcccgagctt gcgcaggttc
    21781 acctcgagca gcgtgtaggt gccgccgtag agcgtcgcgg ccgagacgat gtgatcgccc
    21841 gcctcgcaca gggtcaggat cgcggtcatc tccgccgcct gtccggtcgc ggtcgcgagc
    21901 gccgcgcggc cgccctcgag cgccgccatg cgctcctcga acactgcggt ggtcgggttc
    21961 gacaatcgcg tatagacgtt gccgaaggtc tgcaggttga aaaggctcgc cgcgtgatcg
    22021 gccgagtcga acacgtagga ggtcgtctgg tagagcggca ccgcgcgcgc cccggtcgcg
    22081 gggtcgggga tctgcccggc gtgcagcgcg agcgtctcga tgccgaactt gcggtccgcc
    22141 atggctagcg cgccgtcagt agggcagcca ctgcggacgc ttcgcggaga tcacgcgcgt
    22201 gttcacgccg caccagttca gcccgccgaa caggcgtccg tgctcgatct tcacgcagcg
    22261 gtcgatcacg gcctcgaggc cggcggcctc cgccttcctc gcggcttcca ggttgacgac
    22321 gccgagctgc tgccacagca ccttggcgcc gacggcgatc gcctcgtcgg cgatcggcat
    22381 gatgtcctcg gtcttgcgga agcaatcgac catgtcgatc gcgagccccg acttcgccgc
    22441 cgcctcgcgc accgacttgt agcatttctg accgaggatc tccgactgcc cgggattcac
    22501 gggaatcacc gtgtagccgt gctcgagcag gtatttcgcc gcgaaatagc tcggccgatg
    22561 ccagctcgcc gacaggccca ccatggcgat gacgcgcgtc gtcttcaata tccggcgcag
    22621 cgaagggatg tcgtcgacca tggcccggat tctaacggag gcctacgcgg ggtgccgaag
    22681 ggcactcccg ttcctgaatt gttgatgcgg ggtgaatgga gcatttccga atgcctttga
    22741 atctttcctg cgccgcggtt acgaactagt acgcaggccg cagacgcggc cttcgctatt
    22801 cccgaaagga gcgtatcgaa tatgaagcgc aagctcgcta tgattttcgc cggcagcgcg
    22861 gtcctcggca ccctggcgtt cgcccccgcg gcgttcgcgc aggataccgt gatcgtggac
    22921 cggcccgtga catccgggtc gacgacggtg gtcaccccgg cgcccgattc gagcgccgtc
    22981 gtggtcgagc cgaacacggt ggtggccgcg gcgcctcagc cgcaggcctt cgagagccag
    23041 tatcactacg gcttcgccgc ggcgcgccag cccggcagcg tcccgtcgcc ttacagcgat
    23101 aggccctcgt cggggcatat cggcgacgag ttctactaca agcagttcgg cggcgtctct
    23161 cctccgtaac gacgcctgag gccggcggcc cggttcaacc gaggccggcc ggagccgatt
    23221 ccggcgcacg cgagggttct ccctcgtgcg gcgccgtttt cttgttcccg tccgacgatc
    23281 ggcgccattg gtcccagtac gggtccgggc cgtacttgtg cccgagaaaa tcgacgaagg
    23341 cgcggacctt ggccgagagg tgccggcggc tcgggtacac cgcgtagatc gccgattcgg
    23401 gcgtctcgca atcggcgagc acggcctcca ggcggccctc ctcgagctcc cggccgacgg
    23461 cgaacgtcgg cagcaggacg acaccgatgc cgccgagcgc tgcggcgcgc agcgcgtcgc
    23521 cgctgttcgc gcggatgctg ccgttcgact tgacggcgtg ccgtcggccg tcggcgctgc
    23581 cgaacgtcca ggcgccctgg ctggcgcaga acgaataccc gaggcagttg tgccgctcga
    23641 gatccgccgg gctgcgcggc gtgccgtggc gctggaagta ctcgggcgag ccgcacacca
    23701 ccatgcggca gggcgcgagc ttgcgcgcga ccagcgacga ttcgggcagc gtcgcggcga
    23761 tgcgcactgc gacgtcgaat ccctcctcga cgagatcgac gaagcggtcg ttcatcgtca
    23821 ggtcgagctt gaggtccggg tgcgaggcga ggaactcggg aaggaagggc gcgacgtgcg
    23881 cggttgcgaa gctgtagggc gcattgatgc gcagcgtgcc gcgcggctcg gcgctcgcct
    23941 ggcgcgcctg cgcctcggcc tcctcgagct cgttcaggat ctgcgtgcag cgctcgtaat
    24001 acccggcgcc gctctccgtg agcgagaggc gccgcgtggt gcggttgaga agccggatgt
    24061 tgagatggtt ctcgagattc atcacgtgct tcgtgaccat cgctcgcgac aggcgcagct
    24121 tgccggccgc ggccgcgaag ctgccgttgt cgacgacggc gcagaaaacc ctcatgctgg
    24181 tcaacgaatc catgctcgca gtgcccggac aatcgcttga attcagtggg aatcggaagg
    24241 cgtgggccga gcggatcagc ccagaattgc cgccggccga acgtctgaca cccgttggaa
    24301 agagcgaggc cgctcaagtt caacaatagt cccgattgac cacggcgcgc cgctcgccac
    24361 tcgctagaat cgggtcctcg gtagaatcgg gccttttacg ggaggcgcgc gtgcgatcgg
    24421 gtgaacggac gtcggcgcag atcctcgtcg atgcgctcag gatccacggg acgtcgacgg
    24481 ctttctgcgt ccccggcgag agctatctcg ccgtgctcga cgcgctctac gaggcgcgcg
    24541 acgcgattcg gctcatcgtg tgccgccagg acggcggcgc cgcctacatg gccgaggcct
    24601 acggcaaggt caccggtcag cccgggatct gcttcgtgac gcgcggtccc ggggcgacca
    24661 atgcgtcgat cggcgtgcat accgcgtatc aggactcgac gccgatgatc ctcttcgtcg
    24721 gccaggtgcc cggcgagttc gccgagcgcg aggccttcca ggagatggac tatcggcgca
    24781 tgttcggccc gatgacgaaa tgggtggcgc aggtcgacag gcccgagcgc atgcacgagt
    24841 atgtcagcca cgcgttccac cttgcgatgt cgggaaggcc cggacccgtg gtgctcgcac
    24901 tgcccgagga catgctcacc gagcgcggcg cgccggcgga cgtcgcccgc taccaggtgg
    24961 tgcaggcgag cccggccgcc gcggacgtgg agcgcgtgcg cgcgatgctc gggcgcgccg
    25021 agcggccgct cgcgatcctg ggcggcagca tctggggtga gcaggcccgc cgcga
//
LOCUS       DATDMW010000377         3078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2354371, whole genome shotgun sequence.
ACCESSION   DATDMW010000377 DATDMW010000000
VERSION     DATDMW010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354371"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3078
                     /locus_tag="VI319_15925"
     CDS             <1..>3078
                     /locus_tag="VI319_15925"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY6865381.1"
                     /translation="DAIEVALTDEPGQVPKQSSPPRLALPLGVRLERADVAEITIRRP
                     GSDLALRRLVFAYEGGPAGHRVDGLTVETPWGDAALHAEIGAAEPFVVSGAGALARAD
                     PRLPLGAAIEWKGNLHGLVASLRGGAAGVAVRARAELAPLDARPLRTLEASADGADLA
                     RFGDGLPATALHVELRARGDRGTLLTGNLSAVNARPGAIDAGRLPLARLDARFTADLD
                     SVRLSDLVARSAGGTLSGAGELTRTEARLALHAAGLDLRELHSALKRTRLDGPLDVAV
                     SAERQSLRGTLAQGDLSVSADVVKRGARVDVRSLRAAASGGEAKGRLQLALGGAMPVA
                     GDLAFSRFDPSRFGDYPSGAIDGSVAVRGRLGARRRVETRWTIAPSRLAGLPLESAGS
                     ARLEGERLGDISAEVRLGRSRISARGAFGGKGDRLAWVVDAPVLAEIDPHLSGRVHAE
                     GGATGSWRDPRVQFTARADALRLPGGVAVRSLSAKGAVGKDRTAPLDVALVGHDIDAR
                     GIAVGRLEARSTGSVAAHEASVLAETVGAQAFRVSARLRGGWNDAEGWSGEILELSNE
                     GRYPLRLLRPVTVRASAVHVDVGRVEATLGEGRLVARESEWTPGRLSSSGEFDGLPAA
                     WLLGPAGLAGKIESTLTLEGRWALRSDPALNGTVSVRRGGGDLIVAGTPRIEAALERL
                     VLDAVFRDGAVSADLAVASRLATLSAHAEARPEPGADAPGAASPLSFQAHADVMQLGT
                     VAKPFLTQARVDGRIGIDVQGRGTLAEPVITGSVRGDAIRVDVPPYGVYLKEGKLAAV
                     LEGERVRIEELSIRGGDGSITATGALPLRGAVGGKLDWRAQNLGVLDRPDMRLVVSGQ
                     GAVALEDGRVAVSGDLRASSGFFDIERDRLPDLGSDVVVLGQGAPAREALKRPPVALD
                     VRLDLGDRLTVQGLGFDGKVAGQVRVSTDKAGDLRAEGKLTAKDATYLAYGQALSVDP
                     GEVIFDGPIENPALNITAWRRNQAVEAGVQISGTARNPRAQLVSNPSVPDGEKL"
BASE COUNT          371 a         1097 c         1220 g          390 t
ORIGIN      
        1 gacgcgatcg aggtcgcgtt gacggacgag cccgggcagg tgccgaagca gtcttcgccg
       61 ccgcggctcg cgctcccgct cggcgtgcgc ctcgagcgcg ccgacgtggc cgagattacg
      121 atcaggagac cgggatccga cctcgcgttg cgccggctcg tgttcgccta cgaaggcggc
      181 ccggcgggcc atcgcgtcga cgggctgacc gtggagacgc cctggggcga cgcggcgctg
      241 cacgccgaga tcggcgccgc agagcccttc gtggtctcgg gcgcgggcgc gctcgcgcgc
      301 gccgacccgc gccttccgct cggcgccgcg atcgaatgga aggggaacct ccacggcctg
      361 gtcgcgtcgc ttcgcggcgg cgccgcgggg gtcgcggtgc gggcgcgcgc ggagctcgcg
      421 ccgctcgacg cgcgtccgct gcggacactc gaagcgagcg cggacggcgc ggacctggcg
      481 cggttcggcg acggcctgcc ggcgaccgcg ctgcacgtcg agctgcgggc acgcggcgat
      541 cgcggcacgc tgctgaccgg gaacctgtcc gcggtcaacg cgcgccccgg ggcgatcgac
      601 gccgggcggc ttccgctcgc gcgtctcgac gcgcgcttca ccgccgacct cgacagcgtg
      661 cggctgtcgg acctggtcgc gcggtcggcc ggcgggacgc tctcgggcgc aggcgagctc
      721 acgcgcaccg aggcgcgcct cgcgctgcac gcggccgggc tcgacttgcg cgagctgcat
      781 tccgccctca agcgcacgag actcgacggg ccgctcgacg tcgcggtctc cgcggagcgc
      841 cagtcgctgc gcggcacgct cgcgcaaggc gatctgagcg tcagcgccga cgtggtgaag
      901 cgcggcgcgc gagtcgacgt gcgctcgctg cgcgcggccg cgagcggcgg cgaggcgaag
      961 ggcaggctgc agctggcgct cggcggcgcc atgccggtcg ccggcgacct tgcgttctcg
     1021 cgcttcgatc cttcgcgctt cggcgattac ccgtcgggag cgatcgacgg cagcgtcgcc
     1081 gtccgcggcc gattgggcgc gcggcgccgc gtcgaaacgc gctggacgat cgcgcccagc
     1141 cggctcgccg ggctgccgct cgagagcgcg ggatcggctc gcctggaggg agagcgcctg
     1201 ggcgacatca gcgccgaagt gcggctcgga cgatcgcgca tctcggcgcg cggcgccttc
     1261 ggcggcaagg gggaccggct cgcatgggtc gtcgatgcgc cggtccttgc cgagatcgac
     1321 ccgcacctgt cggggcgcgt gcacgccgag ggcggcgcga cgggaagctg gcgcgatccg
     1381 cgcgtgcagt tcaccgcgcg ggccgacgcg ctgcgcctgc ccggcggcgt cgcggtgcgc
     1441 tcgctctcgg cgaaaggcgc cgtgggaaag gaccgcaccg cgcctctcga cgtcgcgctg
     1501 gtcggtcacg acatcgacgc acgcggtatc gcggtcggac gcctcgaggc gcgcagcacg
     1561 gggagcgtcg ccgcgcacga ggcgagcgtg ctcgccgaga ccgtgggggc gcaggcattc
     1621 cgcgtgtcgg cgcggctgcg cggcggctgg aacgacgccg agggctggtc gggcgagatc
     1681 ctcgagctga gcaacgaggg gcgctatccg ctgcggctgc tgcgtcccgt gaccgtgcgc
     1741 gcgtcggcgg tgcacgtcga cgtgggacgc gtcgaggcga cgctcggcga gggacgcctc
     1801 gtcgcgcgcg aatcggagtg gaccccgggg cgattgtcct cgagcggcga gttcgacggg
     1861 cttccggcgg catggctgct gggacccgcg ggcctcgccg ggaagatcga gtcgacgctc
     1921 acgctcgagg gccggtgggc gctgcgctcg gatcccgcgc tgaacggcac ggtgtcggtg
     1981 cgccgcggcg gcggcgacct catcgtcgcg gggacgccgc ggatcgaagc cgcgctcgag
     2041 cggctcgtgc tcgatgccgt gttccgcgac ggcgcggtgt cggccgatct cgcggtcgcg
     2101 tcgcggctgg cgacgctgtc ggcgcacgcc gaggcccggc ccgagcccgg cgccgacgcg
     2161 ccgggtgcgg cgtcgccgct ctcgttccag gcgcacgccg acgtgatgca gctcggcacg
     2221 gtggccaagc ccttcctcac gcaggcccgg gtcgacggca ggatcggcat cgacgtgcag
     2281 ggtcgcggga cgctcgccga gcccgtgatc acgggcagcg tgcgcggcga tgcgatccgc
     2341 gtcgacgtgc cgccgtacgg cgtctatctc aaggagggca agctcgcggc cgtcctcgag
     2401 ggcgagcgcg tgcgcatcga ggagctatcc atccgcggcg gcgacggcag catcacggcg
     2461 acgggtgcgc tgccgctgcg cggcgcggtc ggcgggaagc tcgactggcg cgcgcagaac
     2521 ctgggcgtgc tcgaccggcc cgatatgcgc ctcgtggtgt cggggcaagg cgccgtagcg
     2581 ctcgaggacg ggcgcgtcgc ggtatccggc gatctgcgcg cgagctcggg cttcttcgac
     2641 atcgagcgcg accgcctgcc cgacctcggt agcgacgtgg tcgtgctcgg ccagggcgca
     2701 ccggcgcgcg aggcgctcaa gcggccgccc gtcgcgctcg acgtccggct cgatctcgga
     2761 gaccgtctca cggtccaggg cctgggcttc gacggcaaag tcgcgggcca ggtgcgggta
     2821 tcgaccgaca aggcgggcga cttgcgcgcc gaaggcaagc tcacggcgaa ggacgcgacc
     2881 tacctcgctt acggccaggc cctgagcgtc gacccgggcg aggtgatctt cgacgggccg
     2941 atcgagaacc cggcgctgaa catcacggcc tggcggcgca accaggcggt cgaggcgggc
     3001 gtgcagatca gcggcaccgc gcgcaacccg cgcgcgcagc tggtctcgaa tccgtcggtc
     3061 ccggacggcg agaagctc
//
LOCUS       DATDMW010000378         3146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1481534, whole genome shotgun sequence.
ACCESSION   DATDMW010000378 DATDMW010000000
VERSION     DATDMW010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481534"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VI319_15930"
     CDS             complement(<1..586)
                     /locus_tag="VI319_15930"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY6865382.1"
                     /translation="MIAAAPAATSFSFEPLFAVLAAAALYGYVRLARTVERPSAWRAT
                     LFGLGLVLVAVPLNSPLETIAIHYLLVVHLLQNVMIADWAPPLLVLGLTPAMRAAVAA
                     RGGRRLAFATRPRFALPFWLAVWYGVHLPGFYDFALRHPLLLNVEHLLLLTAGLLFWW
                     PVFSELPQRLSTALKLAYLGAAYVGSAFLSLALIF"
     gene            complement(583..1392)
                     /locus_tag="VI319_15935"
     CDS             complement(583..1392)
                     /locus_tag="VI319_15935"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY6865383.1"
                     /translation="MLVAHVSSSSWQAPPAVLIGAALAAALFVQAFVRLRSRRPDLAP
                     WSRAVLFGAGLALLVLPLVSPLDHLGDEELLSAHMLQHVLIGDAAAALLVVAVRGPLL
                     FFLLPPPLLKPLASFRPLRSLLAVLLVPLVSLGIWAVVILAWHVPAVYDYAAAHPLVH
                     DLQHLSFVVAGVLVWTQLVDPARHGRLNRPQRVFFALAMLVIAQPLVDLLLFTPPAYD
                     RYVGAHGISAQTDQRLAGAVMMVEQLLTLGICVALLLRPYVRERLRKPAFT"
     gene            1450..2286
                     /locus_tag="VI319_15940"
     CDS             1450..2286
                     /locus_tag="VI319_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865384.1"
                     /translation="MRRFLLLPCALVVLAGCSSSSRPSAPTVAPARTFSLSGFRPSRV
                     AAPGPATVAFTIRQPSGQPLTSYRRGPGPHTGVHLIVVRRDLGTIIHRHPPISADGRV
                     SETIDFPAPGPYRVLVDAYPAGRGLPRNFQLHEDVRVGGRYRPQPLPPFRSSQTVDGY
                     RIDLRAPRTLRAIEAATLVASVRDPSGKPVVFKPWFGALAHAIFFRAGPLDYFHTHVC
                     GPDTPGCTSVLGGARVVGRPAGPGKLNVGVLLPVAGTWRLFLQFRDRGRIVTAPFTLK
                     VR"
     gene            2283..2897
                     /locus_tag="VI319_15945"
     CDS             2283..2897
                     /locus_tag="VI319_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865385.1"
                     /translation="MKARLLLWPFAVALLVLTARTLAYALAPSPLARALGRSAGGPGL
                     VTVTLVSLGLAVTASVLVVWLASLGVRERARLRPERVAPAIRLRRAACRTVALHAASS
                     LVFAVFESYLHWRAGLGFHGLSCLVGPVHRNAIPLLAALSLLAAALAEAAGHVLAWMR
                     AVARELRRRRLVPLPHPPRFAAPPAPLVRAVSARPRSRAPPLPA"
     gene            2926..>3146
                     /locus_tag="VI319_15950"
     CDS             2926..>3146
                     /locus_tag="VI319_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HEY6865386.1"
                     /translation="MRTALLAAAAAAVSLVLASPALAHAIMSPPVAQAKVDQQFTLSV
                     PTEEEGATTTEIELTVPDGFGIDSFEPPTG"
BASE COUNT          434 a         1199 c         1138 g          375 t
ORIGIN      
        1 agaagatcag cgccagcgag aggaaggccg acccgacgta ggccgcgccc aggtaggcga
       61 gcttcagcgc ggtcgagagg cgctgcggca gctccgagaa gaccggccac cagaagagca
      121 ggcctgccgt cagcagcagc aggtgctcga cgttcagcag cagcgggtgg cgcagggcga
      181 agtcgtagaa gccgggcagg tggacgccgt accagaccgc gagccagaac ggcagcgcga
      241 agcgcggacg ggtcgcgaac gccagccggc ggcccccgcg cgcggccacc gcggcccgca
      301 tcgccggcgt caggccgagc acgagcagcg gcggcgccca gtcggcgatc atcacgttct
      361 gcagcaggtg cacgacgagc aggtagtgga tcgcgatcgt ctcgagcggc gagttcagcg
      421 gcaccgcgac gagcacgagc ccgaggccga agagggtcgc ccgccacgcg gaggggcgct
      481 cgaccgtgcg cgccaggcgg acgtagccgt acagcgccgc cgcggcgagc acggcgaaga
      541 gcggctcgaa gctgaacgag gtcgcggcgg gcgcggcggc gatcacgtga aggcgggctt
      601 gcgcagccgc tcgcggacgt acggccgcag cagcagcgcg acgcagatcc cgagggtcag
      661 cagctgctcg accatcatca cggccccggc gagccgctgg tcggtctgcg ccgagatccc
      721 gtgtgcgccg acgtagcggt cgtaggcggg cggggtgaac agcagcaggt cgacgagcgg
      781 ctgcgcgatg accagcatcg cgagcgcgaa gaacacgcgc tgcggacgat tcagacggcc
      841 gtgccgcgcc gggtcgacga gctgcgtcca gaccagcacc ccggccacga cgaagctcag
      901 atgctggagg tcgtgcacga gcgggtgcgc ggccgcgtag tcgtagaccg cgggcacgtg
      961 ccaggccagg atcaccaccg cccagatgcc gaggctgacg agcggcacga gcagcaccgc
     1021 gagcaacgac cgcagcgggc ggaacgacgc gagcggcttc agcagcggcg ggggcagcag
     1081 gaagaacagc agcggcccgc gcaccgcgac gacgagcagc gcggcggcgg catcgccgat
     1141 caggacgtgc tggagcatgt gcgccgagag cagctcctcg tcgccgaggt ggtcgagcgg
     1201 cgagacgagc gggagcacca gcagcgcgag cccggccccg aacagcacgg cgcgactcca
     1261 cggcgcgaga tcgggccggc ggctccgcaa ccggacgaac gcctgcacga acagcgcggc
     1321 cgcgagcgcc gcgccgatga ggaccgcggg cggcgcctgc caggaggagc tggagacgtg
     1381 cgcgacgagc accgcgggtc agggtaaccg tttgacacgc actcctacgc gtcgtgatcc
     1441 tccctgcaga tgaggcgctt cctgctcctg ccctgcgcgc tcgtcgtcct cgccggctgc
     1501 tcgtcctcgt cccggccgag cgcgccgacg gtggcgcccg cacggacgtt ctcgctctcg
     1561 ggctttcggc cttcgcgcgt cgccgcgccc ggccctgcga cggtcgcgtt cacgatccgc
     1621 caaccctcgg ggcagccgct gacctcgtac cgccgcggcc ccggcccgca cacgggggtc
     1681 cacctgatcg tcgtgcggcg cgacctcggc accatcatcc accggcaccc gccgatctcg
     1741 gccgacggac gcgtgagcga gacgatcgac ttcccggcgc ccgggcccta tcgggtgctc
     1801 gtcgacgcct accccgcggg cagggggctg ccccgcaact tccagctcca cgaggacgtg
     1861 cgggtcggcg gccgctaccg cccgcaaccg ctgccgccgt tccggtcgag ccagaccgtc
     1921 gacggctacc gcatcgacct gcgcgcaccg cggactctgc gcgcgatcga ggcggcgacc
     1981 ctcgtcgcga gcgtccgcga cccgtcgggg aaaccggtcg tgttcaagcc gtggttcggc
     2041 gccctggcgc acgcgatctt cttccgcgcc ggcccgctcg actatttcca cacgcacgtc
     2101 tgcggcccgg acacgcccgg ctgcaccagc gtgctgggcg gcgcgagggt cgtcgggcgc
     2161 cccgccggtc ccgggaaact gaacgtcggc gtgctgctgc ccgtcgcggg cacgtggcgc
     2221 ctgttcctgc agttccgcga ccgcggccgc atcgtcaccg cgccgtttac gctgaaggtt
     2281 cggtgaaggc ccgcctcctg ctctggccgt tcgcggtcgc gctgctcgtg ctcaccgcgc
     2341 ggacactcgc ctacgcgctc gcgccgagcc cgctcgctcg cgcgctcggg cgctccgcgg
     2401 gcgggcccgg gctcgtaacc gtcacgctgg tctcgctcgg cctcgccgtc accgcctcgg
     2461 tgctcgtcgt ctggctcgcc tccctcggcg tccgcgagcg cgcgcgcctc cggcccgagc
     2521 gcgtcgcgcc ggcgatccgg ctgcgtcgcg cggcctgccg cacggtcgcg ctccacgccg
     2581 cgagctcgct cgtgttcgcc gtgttcgagt cgtacctgca ctggcgggcc ggtctcggct
     2641 tccacggcct ctcgtgcctc gtcggcccgg tgcaccggaa cgcgatcccg ctgctggccg
     2701 cgctctccct gctggcggcc gcgctggcgg aggccgccgg ccacgtgctc gcgtggatgc
     2761 gcgcggtcgc gcgggagctg cgccgccgcc ggctcgtccc gcttccgcac ccgccccgct
     2821 tcgcggcgcc ccccgcgccg ctcgtgcgcg ccgtctccgc gcgcccgcgg tctcgcgcgc
     2881 cgccgctgcc ggcctgaccc ggaccggcaa cgcgaaagga gacctatgag aaccgcgctg
     2941 ctcgccgctg cggcggccgc agtcagcctc gtgctcgcga gccccgcgct cgcgcacgcg
     3001 atcatgagcc cacccgtcgc ccaggcgaag gtggaccagc agttcacgct ctccgtcccg
     3061 accgaggagg agggcgcgac gacgaccgag atcgagctga ccgtcccgga cgggttcggc
     3121 atcgactcgt tcgagccgcc gaccgg
//
LOCUS       DATDMW010000379         3707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1607618, whole genome shotgun sequence.
ACCESSION   DATDMW010000379 DATDMW010000000
VERSION     DATDMW010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607618"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VI319_15955"
     CDS             complement(<1..782)
                     /locus_tag="VI319_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6865387.1"
                     /translation="MNSAAIVHPALVEVSDLAYSYGDRPVLKGVSFTIPRGKIVAILG
                     ATASGKSTLLALIGGQLEPRRGSVKIEGRVVHELSTGELYRLRRRIGMMFQAGGLFSD
                     LSVFDNIAFPLRENTALPEPIIREIVLMKLQAVGLRGARDLMPAEVSGGMSRRIALAR
                     AIAMDPMLTMYDEPFAGLDPIALAIVGRLIRRLNDALGATSIVVSHDIVEAMQMVDYL
                     YFISDGVIMGEGTPEEIRRSELPFVKQFVHGETDGPVPFHYPA"
     gene            832..2196
                     /locus_tag="VI319_15960"
     CDS             832..2196
                     /locus_tag="VI319_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY6865388.1"
                     /translation="MASETAALESRTPHTQSGMSALDTEAYWMPFTHNRYFKQHPRLL
                     VRAEGAYYWTADGKRLFDCLSGLWCCPLGHAHPKIADAVARQVKELDYAPAFQMGHPK
                     IFSLARRIAGMAPEGMTHVFFGNSGSEAVDSALKIAIAYHRMKGQASRTRMIGRERGY
                     HGVGLGGTAVGGMVNNRKMFAPLLFAGVDHLPHTYDVTQMAFSRGQPAWGAQLADELE
                     RIVALHDASTVAAVIVEPMQGSGGVIVPPVGYLERLRRICDRHGILLIFDEVITGFGR
                     CGDAFGAQRFGVVPDMITFAKIVTNGVIPMSGVIVKKEIYDAFMTGPEQAVELFHGYT
                     YSGHPVAAAAAHATLDALEEQNAFARVRELEPVLERAVHALRGEPGVVDVRNIGLAAG
                     IDLEPIPGKPGERAFGVFRRGMDNGILLRWSGDTIAVAPPFISTREEVETMVEKLRAS
                     LRGG"
     gene            complement(2197..>3707)
                     /locus_tag="VI319_15965"
     CDS             complement(2197..>3707)
                     /locus_tag="VI319_15965"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY6865389.1"
                     /translation="LLASIAWTGHAAAEEGADRIVHLSADVLHLIAAGAWVGALWPLA
                     AALARDSDGARAARRFSALGIACVATLIATGIANAWYTVGSLPAMIGTPYGRLLTAKL
                     TLFAAMLALASVNRLRLTPRLESGDAPARARLRRNAAIEAALGLAVIALVGVLGATVP
                     ALHEQTVWPFAYTLEVPEGAEAMVGVLFVAPLFALGLVVAGARARRHALVGAGAAVVV
                     AALAAGLWLAAAPAHPTTYFRSPAPYAADPIARGAGIYGVRCAICHGARGRGDGPAAA
                     SLPQRPTDLAAHFSLHREGDLYWWIEHGIPGTAMPGFAQTVPGSAAWDVLEFLRAQSD
                     AADADVLGAAVRPGIGLAAPDFAFQVGTRPQESLGELRGGMVLLVFYELPGSLARLRA
                     LAGVGLHFAHLGVRVIAFPVGGRAAKPGAEGDADMLADPDARVAAAYAPFAPGGAARP
                     VEYLVDRQGDIRARWTPGAVPDWSAMPVLLEQIETLEREPPRPPLARRRHAH"
BASE COUNT          560 a         1369 c         1324 g          454 t
ORIGIN      
        1 gcaggatagt ggaacggcac gggcccgtcg gtctcgccgt gcacgaactg cttcacgaac
       61 ggcagctcgg agcggcggat ctcctccggc gtgccctcgc ccatgatcac gccgtcggaa
      121 atgaagtaga ggtagtccac catctgcatc gcctcgacga tgtcgtgcga gaccacgatg
      181 gacgtcgcgc cgagcgcatc gttcaaccgg cggatgaggc ggccgacgat ggcgagcgcg
      241 atcgggtcga ggcccgcgaa cggctcgtcg tacatcgtga gcatcgggtc catcgcgatc
      301 gcgcgcgcga gcgcgatgcg ccgcgacatc ccgcccgaga cctcggccgg catcaggtcg
      361 cgcgcgccgc gcaggccgac cgcctgcagc ttcatcagca cgatctcgcg gatgatcggc
      421 tcgggcagag cggtgttctc gcgcagcggg aacgcgatgt tgtcgaacac cgagagatcg
      481 gagaacaggc cgcccgcctg gaacatcatg ccgatgcgcc ggcgcaggcg gtacagctcc
      541 ccggtggaga gctcgtgcac cacccggccc tcgatcttca cgcttccgcg ccgcggctcg
      601 agctggccgc cgatcagcgc gagcagcgtc gacttgcccg acgcggtcgc ccccaggatg
      661 gcgacgatct tcccgcgcgg gatcgtgaag ctgaccccct tcaggaccgg gcggtcgccg
      721 tacgagtacg cgagatcgga gacttcgacg agggcgggat ggactatcgc ggcggaattc
      781 atcttcttcc gacctaattt aacataggag gcacccgaag gagaccgcgc catggcatcc
      841 gagaccgccg cactggagtc ccgcaccccg cacacccaat ctggaatgag cgcgctcgac
      901 accgaggcgt actggatgcc gttcacccac aaccggtact tcaagcagca cccgcggctg
      961 ctggtgcgcg ccgagggcgc ctactactgg accgccgacg gcaagcgcct gttcgactgc
     1021 ctctcgggcc tgtggtgctg cccgctcggc cacgcccacc cgaagatcgc cgacgcggtc
     1081 gcgcgccagg tgaaggagct cgactacgcg cccgcgttcc agatggggca cccgaagatc
     1141 ttctcgctcg cgcgccgcat cgccggcatg gcgcccgagg gaatgacgca cgtcttcttc
     1201 ggcaactcgg gatccgaggc ggtcgacagc gcgctcaaga tcgcgatcgc ctaccaccgc
     1261 atgaaaggcc aggcctcgcg cacgcgcatg atcgggcgcg agcgcggcta ccacggcgtc
     1321 ggcctgggcg gcaccgcggt cggcggcatg gtcaacaacc gcaagatgtt cgcgccgctg
     1381 ctgttcgccg gcgtcgatca cctgccgcac acgtacgacg tcacgcagat ggcgttctcg
     1441 cgcggccagc cggcgtgggg cgcgcagctc gccgacgagc tcgagcgcat cgtcgcgctg
     1501 cacgacgcgt cgaccgtcgc cgcggtgatc gtcgagccga tgcagggctc gggcggcgtg
     1561 atcgtgccgc ccgtcggcta cctcgagcgc ctgcgcagga tctgcgaccg gcacggcatc
     1621 ctgctgatct tcgacgaggt catcaccggc ttcggccgct gcggcgacgc gttcggcgcg
     1681 cagcgcttcg gcgtcgtgcc cgacatgatc accttcgcca agatcgtcac caacggcgtg
     1741 atcccgatga gcggcgtcat cgtcaagaag gagatctacg acgccttcat gaccggtccc
     1801 gagcaggcgg tcgagctgtt ccacggctac acctactcgg gccacccggt cgccgccgcg
     1861 gccgcgcacg cgacgctcga cgcgctcgag gagcagaacg ccttcgcgcg cgtgcgcgag
     1921 ctcgagcccg tcctcgagcg cgcagtgcac gcgctgcgcg gcgagccggg cgtcgtcgac
     1981 gtgcgcaaca tcggcctcgc ggccggcatc gacctcgagc cgatccccgg caagcccggc
     2041 gagcgcgcct tcggcgtgtt ccgccgcggc atggacaacg gcatcctgct gcgctggagc
     2101 ggcgacacca tcgcggtggc gcccccgttc atctcgacgc gcgaggaggt cgagacgatg
     2161 gtcgagaagc tgcgcgcgtc cctgcgcggc ggctgatcag tgcgcgtgcc gccgccgcgc
     2221 gagcggcggg cgcggcggct cgcgctcgag cgtctcgatc tgctcgagca ggaccggcat
     2281 cgcgctccag tcgggcaccg cgcccggcgt ccagcgcgcg cggatgtcgc cctgccgatc
     2341 gacgagatac tcgaccggcc gcgccgcgcc gcccggcgcg aacggcgcgt aggcggcggc
     2401 gacgcgcgca tcgggatcgg ccagcatgtc ggcgtctcct tccgcgcccg gcttcgccgc
     2461 gcgcccgccg acggggaacg cgatgacgcg cacgccgagg tgcgcgaagt ggaggccgac
     2521 gccggcgagc gcgcgcagcc gcgcgagcga gccgggcagc tcgtagaaga cgagcagcac
     2581 catgcccccg cgcagctcgc cgagcgactc ctgcggccgc gtcccgacct ggaacgcgaa
     2641 atcgggcgcg gcgagcccga tcccgggccg caccgccgcg ccgagcacgt cggcgtccgc
     2701 ggcgtcggac tgcgcgcgca ggaactcgag cacgtcccac gcggcgctcc ccgggacggt
     2761 ctgcgcgaac cccggcatcg ccgtccccgg gatgccgtgc tcgatccacc agtagagatc
     2821 gccttcgcgg tgcagcgaga agtgcgcggc gagatccgtc gggcgctgcg gcagcgacgc
     2881 cgccgccgga ccgtcgccgc ggccgcgcgc gccgtggcag atggcgcagc gcacgccgta
     2941 gatcccggcg ccgcgcgcga tcggatccgc ggcatagggc gccggggacc ggaagtaggt
     3001 cgtcgggtgc gcgggcgcgg ccgcgagcca gagcccggcg gcgagcgcgg cgaccacgac
     3061 cgcggctccg gcgccgacca gcgcgtgccg gcgcgcgcgc gcgccggcga ccacgagccc
     3121 gagcgcgaag agcggcgcga cgaacagaac gccgaccatc gcctcggcgc cttcgggcac
     3181 ctcgagcgta tacgcgaacg gccacaccgt ctgctcgtgc agcgcgggaa ccgtggcgcc
     3241 gagcacgccg acgagcgcga tgacggcgag cccgagcgcg gcctcgatcg ccgcgttgcg
     3301 ccgcaggcgc gcgcgcgccg gcgcgtcgcc gctctcgagc cgcggcgtga gccgcagccg
     3361 gttgaccgag gcgagcgcga gcatcgccgc gaacagcgtg agcttggcgg tgaggagccg
     3421 gccgtacggc gtgccgatca tggcgggcag gctgccgacc gtgtaccagg cgttggcgat
     3481 tccggtggcg atcagcgtcg ccacgcaagc gatcccgagc gccgagaaac ggcgcgccgc
     3541 gcgcgctccg tcggaatccc gggcgagcgc cgccgccagc ggccacagcg cgccgaccca
     3601 cgcgccggcc gcgatcaggt gcagcacgtc ggccgagaga tggacgatgc ggtcggcgcc
     3661 ctcctcggcc gcggcgtgcc cggtccacgc gatcgaagcg agcagcg
//
LOCUS       DATDMW010000380         3129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2105025, whole genome shotgun sequence.
ACCESSION   DATDMW010000380 DATDMW010000000
VERSION     DATDMW010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105025"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VI319_15970"
     CDS             complement(<1..279)
                     /locus_tag="VI319_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865390.1"
                     /translation="MRWVLRGIAVAAVVAAVLAGAVSWLLHSETAWRWALERAQSSSG
                     GALRIEGERGTPARELRFDRLVYAGPGFRVEVRGVVLRGARLSGAAAGR"
     gene            complement(288..2060)
                     /locus_tag="VI319_15975"
     CDS             complement(288..2060)
                     /locus_tag="VI319_15975"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY6865391.1"
                     /translation="MPTMVAWWTLRALIAVAALAFGLARAATSPEAAPLRYRIELQGP
                     KDLTGMLKDGLKLSRWQHDPGMTPDLLERLLEESVRDAREAVATEGYFSAAVRGTVDR
                     SADPWLVTLRVDPGPRTTVERVDIRVIGPAADDAEAQGMLSRIRARWSLAPGQPFRQE
                     AWESAKRDAVRDLSGWRYAAARVSASRAEIDPQSRGAVLSVELASGPPFRFGPLQVSG
                     TKRYPQKLVDNLDPIRPGDVYDRKALATYQRRLLETGYFVSAQVDVDTEAPDPAAAPV
                     RVALIEGSRQHVEAGVSYSTDAGQRLELRYNNMDALGTASRFKSALQLDRKIQELNLN
                     LDSPPLSEARWNSGFARARSTDIQNQQTAEAAVGVSHNWLSGGTPSAIELSAHTEDQK
                     VEDAPGQRSHTVYLAFRRTFRDTDDVVAPRSGILANWEVGAAPAVLATRGFARATAGA
                     SYFFPLARNDDLLLRATGGVVRAAARDGIPSTFLFRTGGDQTVRGYAFESLGVKQGDA
                     VVGGRALAVTSVEETHWFGASWGVAAFVDAGNAWDRGTPFHAAVGYGAGARFRTPVGP
                     IRIDLAYGRDTHKVRLHFSAGFTF"
     gene            complement(2074..2565)
                     /locus_tag="VI319_15980"
     CDS             complement(2074..2565)
                     /locus_tag="VI319_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865392.1"
                     /translation="MTDVGETIIHVMLWGFVATGAQAAVLFGSQRLGYSRLSLPFLIG
                     TMFTGERPAANAAGAVFYWLGGWLFAFIYYFLFAELGRSNWWAGGLIGTLHGLVLLTT
                     LAPLMPYLHPRMATEYEGPSYRRRLQPPGFLALHYGYRTPLTTLLAHALYGAILGAGM
                     SLH"
     gene            complement(2573..>3129)
                     /locus_tag="VI319_15985"
     CDS             complement(2573..>3129)
                     /locus_tag="VI319_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY6865393.1"
                     /translation="VAMDRLRALDERIGLNLDRGILDRECRAIRDEIERRGFNPRLGA
                     YVGYFDGEEPDASLLLLARHGYIEPGHPRMEGTYRFIDSTLSSHGMLHRYPLDSNYDG
                     IRGHENVFAPCSFWAAEYLANSGRRKEAEALFERLLGCANDVELYAEEIEAATGKPVG
                     NFPQAFTHVSMISAALALYSHKTA"
BASE COUNT          439 a         1122 c         1141 g          427 t
ORIGIN      
        1 gcgccccgcc gcggcgcccg agagccgcgc gccgcgcagc accacgccgc ggacctcgac
       61 gcggaagccc ggaccggcgt agacgaggcg atcgaagcgc agctcgcgcg ccggcgtccc
      121 gcgctccccc tcgatgcgca gcgcgccgcc gctcgaggat tgcgcgcgct cgagcgccca
      181 gcgccaggcc gtctcgctgt gcaggagcca cgagacggcg cccgcgagca ccgcggcgac
      241 cacggcggcg acggcgattc cgcggagcac ccagcgcatc gggcgcctca gaacgtgaac
      301 ccggccgaga aatgcaggcg gaccttgtgc gtgtcacgcc cgtacgcgag gtcgatgcgg
      361 atcggcccga ccggcgtgcg aaagcgtgcg cctgcgccgt agcccaccgc ggcgtggaac
      421 ggcgtgccgc ggtcccacgc gttgcccgcg tcgacgaaag ccgcgacgcc ccagctcgcc
      481 ccgaaccagt gcgtctcctc gacgctcgtc acggcgaggg cgcggccgcc gacgacggcg
      541 tcgccctgct tcaccccaag gctctcgaag gcgtagccgc gcaccgtctg gtcgccgccg
      601 gtccggaaca ggaaggtcga cgggatgccg tcgcgcgccg cggcccgcac cacgccgccg
      661 gtcgcgcgca gcagcaggtc gtcgttgcgc gccagcggga agaagtacga cgcgccggcg
      721 gtggcgcgcg cgaatccgcg cgtggcgagc accgcgggcg cggcgcccac ttcccagttg
      781 gcgaggatgc ccgagcgcgg cgcgaccacg tcgtcggtgt cgcgaaaggt cctgcgaaag
      841 gcgagataga ccgtgtggct gcgctggccg ggcgcgtcct cgaccttctg atcctcggtg
      901 tgcgcggaca gctcgattgc cgacggcgtg ccgcccgaga gccagttgtg cgagacgccg
      961 accgcggcct cggcggtctg ctggttctgg atgtcggtgc tgcgggcgcg cgcgaagccg
     1021 ctgttccagc gcgcctccga gagcggcggc gaatcgaggt tcaggttcag ctcctggatc
     1081 ttgcggtcga gctgcagggc gctcttgaag cgcgacgccg ttccgagcgc gtccatgttg
     1141 ttgtagcgca gctcgaggcg ctggcccgcg tcggtgctgt agctgacgcc ggcctcgacg
     1201 tgctggcggc tgccctcgat cagcgccacg cgcaccggcg ccgccgcagg gtcgggcgcc
     1261 tcggtgtcga cgtcgacctg cgcgctcacg aaatatcccg tctcgagcag gcgccgctga
     1321 taggtggcga gcgccttacg gtcgtagacg tcgcccggcc ggatgggatc gagattgtcg
     1381 acgagcttct gcggatagcg tttggtgccg ctcacctgga gcggcccgaa ccggaacggc
     1441 ggcccgctcg cgagctcgac gctcagcacg gcgccgcgcg actgcgggtc gatctcggcg
     1501 cggctcgcgg agacgcgcgc cgcggcgtag cgccagccgg aaagatcgcg cacggcgtcg
     1561 cgtttcgcgc tctcccaggc ttcctggcgg aagggctgcc ccggcgcgag cgaccagcgc
     1621 gcgcggatgc ggctcagcat gccctgggct tcggcgtcgt ccgccgccgg cccgatcacg
     1681 cggatgtcga cgcgttcgac cgtcgttctc ggccccgggt ccaccctcag cgtcaccaac
     1741 cacggatcgg cgctccgatc gacggtgccg cgcaccgccg cggagaagta gccttcggtg
     1801 gcgaccgcct cgcgggcatc gcggaccgat tcctcgagca ggcgctcgag caggtccggc
     1861 gtcatccccg ggtcgtgctg ccagcgcgag agcttgaggc cgtccttcag catgccggtg
     1921 aggtccttcg gcccctgcag ctcgatgcgg tagcgcagcg gcgccgcctc gggcgaagtg
     1981 gcggcgcgcg ccagcccgaa ggccagagcg gcgaccgcga tgagcgcgcg caaggtccac
     2041 cacgcgacca tcgtcggcat cggctcgccg ggttcagtgc agcgacattc cggcgccgag
     2101 gatcgcgccg tacagcgcgt gcgcgagcag agtcgtgagc ggcgtgcggt agccgtagtg
     2161 cagcgcgagg aacccgggcg gctgcagccg gcggcggtag ctcgggccct cgtactcggt
     2221 cgccatgcgg gggtgcaggt acggcatcag cggcgccagc gtcgtgagca ggacgagccc
     2281 gtgcagcgtg ccgatcagcc cgccggccca ccagttgctg cgtcccagct cggcgaacag
     2341 gaagtagtag atgaacgcga acagccatcc gccgagccag tagaacaccg cgcctgccgc
     2401 gttcgccgcg ggccgctcgc cggtgaacat cgtcccgatc aggaacggca gcgacagccg
     2461 cgagtacccg agccgctggc tgccgaagag cacggcggcc tgcgcgcccg tagcgacgaa
     2521 gccccagagc atgacatgga tgatggtctc gccgacgtcg gtcatgggtg cgttacgcgg
     2581 tcttgtggga gtacagcgcc agggcggccg agatcatgct gacgtgcgtg aacgcctgcg
     2641 ggaagttgcc gaccggcttg cccgtggccg cctcgatctc ctcggcatac agctcgacgt
     2701 cgttggcgca gccgagcagg cgctcgaaca gcgcctccgc ctccttgcgc cggccgctgt
     2761 tcgccaggta ctccgcggcc cagaagctgc acggcgcgaa cacgttctcg tggccgcgga
     2821 tgccgtcgta gttgctgtcg agcggatagc ggtgcagcat gccgtggctc gagagcgtcg
     2881 agtcgatgaa ccggtacgtc ccttccatgc gcgggtgccc cggctcgatg tagccgtggc
     2941 gcgcgagcag cagcaggctc gcgtcgggct cttcgccgtc gaagtagccg acataggcgc
     3001 cgaggcgcgg gttgaagccg cgccgctcga tctcgtcgcg gatcgcccgg cactcgcggt
     3061 ccaggatgcc gcggtccagg ttgagcccga tgcgctcgtc gagcgcgcgc agccggtcca
     3121 tcgcgaccc
//
LOCUS       DATDMW010000381         3773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_611946, whole genome shotgun sequence.
ACCESSION   DATDMW010000381 DATDMW010000000
VERSION     DATDMW010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611946"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /locus_tag="VI319_15990"
     CDS             complement(<1..1876)
                     /locus_tag="VI319_15990"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY6865394.1"
                     /translation="MIALGLAIGVVAWAFAEGEDADRDRDREKDREKEKIQVAIAAPA
                     AGALYAAPAAVHVVALASTRQKRHPVARVEFFAGAARIGVVTGPSATGQYAFDWTGVA
                     AGAYTLTAKATNDKGDSDVSAPVSIVVDALPSVALSAPAANAVFNAPAAVTLSASASD
                     ADGTVSKVEFFQGATLIGTAAGAPYTASWNGAAPGVYALTATATDNLGFSTTSAPVRV
                     ILNAAPTVSVASPRADAKFNAPASIPLRAMATDADGSIVRVDFYQGQVLIGSAGAAPF
                     EFEWTSVAAGKYSVTAVATDDRGATSTSSAVAVSVNAPPAVSITAPADGAVYLAPGTV
                     AITASASDADGAVTKVEFFQGATLIGTATAAPYSVTLSGLGSGTYPITAVATDDAGAS
                     TTSAVIAVRINAAPTVTLTKPADGAKFTAPADITITASAADLDGNVAKVEFFRGDTLI
                     TTLTAAPYSFTLTGVPQGAYSLTAVITDDLGAVTTSDPVSVTVNSGVAQLYYIYPDHL
                     NTPRLIEDQNKNAVWKWDQTEPFASDRPNEDPRNTGGAFTCNLRFPGQYFDQETSGHY
                     NYFRNFDPSLGRFREADPIGLAWSLNLYGYVGGNPIKYRDPKGLFAMYGNWGGRNWSA
                     G"
     gene            complement(2097..>3773)
                     /gene="recQ"
                     /locus_tag="VI319_15995"
     CDS             complement(2097..>3773)
                     /gene="recQ"
                     /locus_tag="VI319_15995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEY6865395.1"
                     /translation="KSLCYQLPALMRADLTVVVSPLVSLMQDQVEGLERAAPGRAALV
                     NAQQDQAANRGAIARAAAGEVKLLHVAPERFSSPGFLEAMRDVGVGLFVVDEAHCVSQ
                     WGHDFRPEYLQLSLLHERFPDVPRIALTATADPQTRSEIIRRLELGRARVFISSFDRP
                     NIRYTIVDKADPRAQLLRFIREEHEGEAGIVYCLSRKKTDETAEWLAGHGVTALPYHA
                     GMEAGARAENQARFQREDGIVMVATIAFGMGIDKPDVRFVAHLDLPKSIEGYYQETGR
                     AGRDGLPADAWMTYGLGDVVQLRRLIEQSEANEEFRRISFAKLDALLGLCETAGCRRV
                     RLLDYFGEKSEPCGNCDTCLAPPETWDATDAARKALSAIYRTGQRFGAVHVIDVLRGH
                     ASARVSQWDHDKLAVFGLGADLDEATWRGVFRQLVAMGYARPDHDAFGGLKLTDAARA
                     VLKGEQAVEMRRAPRRVIKAKRSARRPEWAEQPGAADVDEDLLARLKAWRSAQAKEQA
                     VPAYVIFHDATLAAIAAAQPRDLDALATIAGIGQKKLGRYGEALLEVVRG"
BASE COUNT          565 a         1342 c         1249 g          617 t
ORIGIN      
        1 gaccagcact ccaattcctg ccgccccagt tgccatacat tgcaaagagg cctttcgggt
       61 ctcgatattt aatggggttg ccgccaacat agccgtataa attcagcgac cacgctaacc
      121 caatcgggtc cgcttcgcga aaacgaccca gactcgggtc gaagtttctg aagtagttat
      181 agtgtccgct tgtttcttgg tcgaaatatt gcccagggaa gcgaagattg caggtgaatg
      241 ctccaccagt attcctggga tcctcgttcg gtctatcaga cgcgaatggc tccgtttgat
      301 cccacttcca taccgcattc ttattttggt cttcgatcaa tcttggcgta ttgagatgat
      361 cgggatagat gtaatagagc tgtgccacgc cggagttcac agtaacgctc accggatctg
      421 acgtcgtcac agcgcccaga tcatccgtga tcaccgcagt aagcgagtaa gccccttgcg
      481 gcaccccggt cagcgtaaag ctgtacggcg ccgccgtgag cgtcgtgatg agcgtgtcac
      541 cgcggaaaaa ctcgaccttc gcgacattgc cgtcaagatc cgcggcgctc gcggtgatcg
      601 tgatatcggc aggggccgtg aacttcgcgc cgtccgcggg cttcgtgaga gtgacggtcg
      661 gcgctgcgtt gatgcgcacg gcgatcacgg ccgaggtggt cgaagcaccg gcgtcgtcgg
      721 tcgcgacagc ggtgatcggg taggttccgg agccgagacc cgacaaggtg accgagtaag
      781 gcgccgctgt cgccgtcccg atcagggtcg cgccctggaa gaactcgacc ttcgtcacgg
      841 cgccgtccgc atcgctcgcg ctcgccgtga tcgccacggt gccgggcgcg agatagaccg
      901 ccccatccgc gggcgccgtg atgctcaccg ccggcggcgc attcacgctc accgcgaccg
      961 cgctcgacgt gctcgtcgcg ccgcgatcat cggtcgccac cgcggtgacg ctgtatttcc
     1021 ccgccgcgac gctcgtccac tcgaattcga acggcgccgc gccggccgac ccgatgagga
     1081 cctgcccttg ataaaagtcg acgcggacga tgctgccgtc cgcgtcggtc gccatggcgc
     1141 gaagggggat gctggcggga gcgttgaact tcgcgtccgc tcgggggctg gcgactgaaa
     1201 ccgtcggcgc cgcgttgagg atcacgcgga ccggcgccga tgtggtgctg aatccgaggt
     1261 tgtccgtggc cgtcgccgtc agcgcgtaga cgcccggcgc cgcgccgttc cagctcgctg
     1321 tatacggcgc accggccgcg gtcccgatca acgtggcacc ctggaagaac tcgaccttcg
     1381 agaccgtgcc gtccgcgtcg gacgcgctcg cggacagcgt gaccgccgcc ggcgcgttga
     1441 acaccgcgtt cgccgccggc gcggagagcg ccacgctcgg cagcgcatcg accacgatcg
     1501 acacgggcgc cgagacgtcg ctgtcgccct tgtcgttcgt tgccttcgcc gtcagcgtgt
     1561 acgcgcccgc ggcgacgccg gtccagtcga aggcgtactg gccggtcgcg ctcggacccg
     1621 tcacgacgcc gatgcgcgcc gcgccggcga agaactccac cctcgcgacc ggatggcgct
     1681 tctgccgcgt gctcgcgaga gcaacgacgt gcacggccgc gggcgccgcg tagagcgcac
     1741 ccgccgccgg cgccgcgatc gcgacctgga ttttttcttt ctcccggtct ttctcccggt
     1801 ctctgtccct gtcggcgtcc tcgccctcgg cgaacgccca ggcgacgacg ccgatcgcca
     1861 gcccgagcgc gatcaaagcc agcccggccg tccgcgcaga cactcgcatt cggagccctc
     1921 ccccgacgaa gtgaacggat ttctcggcgc gcgaggcgcc gcggttttcg ttgtaatttc
     1981 caggaaccgg cggttggcgt caatcctgta acttcctaca gggaaaaacc gcgccgaaga
     2041 gattgaatcg ccgagggttc agcgtgcgct gatacggcgc accaccccgc cgcctgctac
     2101 ccgcgcacca cctcgagcag cgcctcgccg tagcggccga gcttcttctg cccgatgccg
     2161 gcgatggtgg cgagcgcatc gagatcgcgc ggctgcgcgg ccgcgatcgc ggcgagcgtg
     2221 gcgtcgtgga agatcacgta cgcgggcacc gcctgctctt tcgcctgcgc gctgcgccag
     2281 gccttgaggc gcgcgagcag gtcctcgtcg acgtcggccg cgcccggctg ctccgcccac
     2341 tcgggccgcc gcgcgctgcg cttcgccttg atcacccggc gcggcgcgcg ccgcatctcg
     2401 accgcctgct cgcccttcag cacggcccgc gccgcgtcgg tgagcttcag tcccccgaac
     2461 gcgtcgtggt cgggccgcgc gtagcccatc gcgacgagct ggcggaagac gccgcgccac
     2521 gtcgcctcgt cgagatcggc gccgaggccg aagaccgcga gcttgtcgtg gtcccactgc
     2581 gacacgcgcg cgctcgcgtg gccgcgcagc acgtcgatca cgtgcaccgc gccgaagcgc
     2641 tgtccggtgc ggtagatcgc cgacagggct ttgcgcgccg cgtcggtcgc gtcccaggtc
     2701 tcgggcggcg cgaggcaggt gtcgcagttg ccgcacggct cgctcttttc tccgaagtag
     2761 tcgagcagcc gcacgcgccg gcagcccgcg gtctcacaca ggccgagcag cgcgtcgagc
     2821 ttcgcgaacg agatgcggcg gaactcttcg ttcgcctccg actgctcgat caggcgccgc
     2881 agctgcacca cgtcgcccag gccgtaggtc atccacgcgt ccgccggcag cccgtcgcgc
     2941 cccgcgcggc cggtctcctg gtagtagccc tcgatcgact tcggcaggtc gaggtgcgcg
     3001 acgaagcgca cgtcgggctt gtcgatgccc atgccgaacg cgatcgtcgc gaccatgacg
     3061 atgccgtcct cgcgctggaa gcgcgcctgg ttctcggcgc gcgccccggc ctccatcccc
     3121 gcgtggtacg gcagcgcggt cacgccgtgc ccggcgagcc actcggccgt ctcgtccgtt
     3181 ttcttgcgcg acaggcagta gacgatgccc gcctcgccct cgtgctcctc gcggatgaag
     3241 cgcagcagct gcgcgcgcgg atcggccttg tcgacgatgg tgtagcggat gttcggccgg
     3301 tcgaagctcg agatgaagac gcgcgcccgc ccgagctcca ggcgacggat gatctcgctc
     3361 cgcgtctgcg ggtcggcggt cgcggtgagc gcgatgcgcg ggacgtccgg gaagcgctcg
     3421 tgcaggagcg agagctgcag gtactcgggc cggaagtcgt gcccccactg cgacacgcag
     3481 tgcgcctcgt ccacgacgaa caggcccacg ccgacgtcgc gcatcgcctc gaggaacccg
     3541 ggcgacgaga agcgctccgg agcgacgtgg agcagcttca cctcgcccgc cgccgcgcgg
     3601 gcgatcgcgc cgcggttggc cgcctggtcc tgctgcgcgt tgacgagcgc ggcgcgcccc
     3661 ggagccgcgc gctcgaggcc ctcgacctga tcctgcatga gcgacacgag cggcgagacg
     3721 acgaccgtca ggtccgcgcg catcagcgcc ggcagctgat agcagagcga ctt
//
LOCUS       DATDMW010000382         6328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_736365, whole genome shotgun sequence.
ACCESSION   DATDMW010000382 DATDMW010000000
VERSION     DATDMW010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736365"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VI319_16000"
     CDS             complement(<1..205)
                     /locus_tag="VI319_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEY6865396.1"
                     /translation="MRQLVLELAPPEPPSFASYVPGRNAAALAALESALAGGERFVYL
                     WGPSGSGKTHLLRAFETAGGRAAD"
     gene            complement(212..1285)
                     /locus_tag="VI319_16005"
     CDS             complement(212..1285)
                     /locus_tag="VI319_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY6865397.1"
                     /translation="MLPARRADREKWWWLALGLGFLLLLYFLSPILTPFLLGGILAYI
                     LNPLVTWLERHRVNRTFGAVIGIALVASVLVLLVLIVVPLFAKEARMLGERLPGALEQ
                     ANARLVPWLHDRFGIDLQLDPASVRDYFADAVKGTEGLIATVLQSLKVGGATLVGILV
                     TVLLIPVVLFYLMRDWDRLIAVVESLVPRTLHPKVSEIARETDVVLAEFLRGQLSVMF
                     VMSFYYSIALWIAGLDFYLPVGVITGILVFIPYVGSFTGLLLATLAGLIQFGPTMGLV
                     WVLVAFGIGQAIEGMGVTPLLVGKRIGLHPVAVIFALLAFGQLFGFFGVLLALPASAA
                     LLVGLRHLRARYIASSVYGGDAG"
     gene            1337..2386
                     /gene="purM"
                     /locus_tag="VI319_16010"
     CDS             1337..2386
                     /gene="purM"
                     /locus_tag="VI319_16010"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY6865398.1"
                     /translation="MASRKPISYRDAGVDIDAGDALVEAIKPLAKRTMRPEVLAGIGG
                     FGALCEIPKRYREPVLVSGTDGVGTKLKLAFRLNRHDTVGIDLVAMSVNDILVQGAEP
                     LFFLDYYACGRLDVGVATQVVAGIARGCDLAGCALIGGETAEMPGMYPEGEYDLAGFA
                     VGVVEKGALIDGRSIAPGDAVLGLASSGAHSNGYSLVRRILDVSGADPGSSFHGRTLA
                     EALLEPTRIYVKPLLALMRAMTVKGLAHITGGGLVENVPRVLPDGTKAVLDRGAWTPP
                     PIFAWLQQQGNVAEAEMLRVFNCGIGMVVVVDERDAARAQEALRAAGETAWRIGRIEA
                     RAQGEAQVVVSRGGT"
     gene            2516..3526
                     /locus_tag="VI319_16015"
     CDS             2516..3526
                     /locus_tag="VI319_16015"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6865399.1"
                     /translation="MRIRFALFTALALLALVGCNRQSQAPYFEGKTLTIVVGYKPGGG
                     YDTVARLLSRYLPKYIPGAPTVIVQNMPGANSLIAANYVYAQAKPDGLTIGTFNRNLP
                     IGQLLKVDGVKFDMLKFAWIGSAASETTVLAVRDDLPYKTFDDMRKAKEPIVVGATGP
                     GANTYDFPLLLKEFLGVNLKLVSGYSSSADIMLAVERKEADARAGSYSSIAPFVERKL
                     VHPVIRSRASEPEIANLPTDESLAPNPRAKAMMALRSAPEVVGRPYVMTPGTPDNLLN
                     ILRDAFVKSLADPDLLAEAKKSDMTLNFVSGDQALEVMKEVLNQPKDVVDEFRKYVKF
                     GE"
     gene            3523..5028
                     /locus_tag="VI319_16020"
     CDS             3523..5028
                     /locus_tag="VI319_16020"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY6865400.1"
                     /translation="MIESALDGLLLVLQWKALAFMGLGILLGFWVGLLPGLGGVTTLA
                     LMLPFIYAMKPHEALAFLLGMHSVVATTGDITSVLFGIPGESTTAAIILDGHSMAKKG
                     EAGRALGAALSSSLLGALVGALALALAIPIVRPLVLAFGSPELFMLAIVGLAFIASLS
                     GHGARGMVRGFIAGGLGLLLATVGQDPQAGVARFTFGTLYLWQGLDIVAVLVGLFAVP
                     EILDLVVRGSSIAGDNTRVGKVGRGVLEGVKDTFHHFGLAIRCSLIGTFIGITPGLGG
                     PVAQWMAYGHAVQSAKTEEERKGFGRGDVRGVIGPGAASNSKEGGSLIPTIAFGVPGS
                     ASMAVILGGFFLMGLVPGPDMLTKNLAITYSMVWTIVLANIITVAACLVFLNRLASLT
                     NVPGELLVPVILVLIFIGAYTANNQYGDLVVMLLCGGIGWLMVVAGWPRAPFVLGLVL
                     GKIAENYLYISVARYHASWLTRPIVVALFAIAVVVICYPIWQARRTRRARA"
     gene            5021..5494
                     /locus_tag="VI319_16025"
     CDS             5021..5494
                     /locus_tag="VI319_16025"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY6865401.1"
                     /translation="MHNKASLALGVVVMLVSAWGVISAWGWPAKAALFPLVICVPLFF
                     LAAAEVLWSLYGASAQSASDFKLSDHLPQSVALRRTAVAAGWILGFFFGILLLGFLIA
                     VPVFVLAYLRVQGKRGWVFSAIFTAVVWAFFFGLFDLLLHLPFPAGWIPSLLGVR"
     gene            complement(5513..>6328)
                     /locus_tag="VI319_16030"
     CDS             complement(5513..>6328)
                     /locus_tag="VI319_16030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6865402.1"
                     /translation="AAMIACAPILVRLSDTSPGASAFWRVALSAPVLALWARAEPGAH
                     RAAPASWTPLVLAGLCFAGDLAAWHVSIVYTSVANATLEANFAPVFVTAGAWLLWRER
                     PSALFVGALGLTAAGAALLIAPNFALGGRTLAGDALGVLTAVFYAGYMLALKSATRGA
                     STARIMAVSTAVAAVVLLPYALATAEVFRAQDARGWLVLAALALVPQVGGQSLIAFGF
                     ARLPASLSAVSLLLQPLLAALYAWLLLGEAMSGMQMAGAAIVLAGIHLARRGR"
BASE COUNT          967 a         2167 c         2263 g          931 t
ORIGIN      
        1 cgtcggccgc gcggccgccg gcggtctcga aggcgcgcag caggtgcgtc ttgccgctcc
       61 ccgaagggcc ccagaggtag acgaagcgct cgccgcccgc gagcgcgctc tcgagcgcgg
      121 cgagcgcggc ggcgttgcgg ccgggaacgt agctcgcgaa gctgggcggc tcgggcggcg
      181 cgagctcgag gaccagttgc cgcacggccg cctagcccgc gtcgccgccg tacacggagc
      241 tcgcgatgta gcgcgcgcgc aggtgccgca ggccgaccag cagcgccgcg ctcgcgggca
      301 gcgcgagcag cacgccgaag aagccgaaca gctgcccgaa cgcgagcagc gcgaagatca
      361 cggcgaccgg gtgcaggccg atgcgcttgc cgacgagcag cggcgtgacg cccatgccct
      421 cgatcgcctg gccgatgccg aaggcgacca gcacccagac gagccccatc gtcgggccga
      481 actggatgag gcccgcgagc gtggcgagga gcagtccggt gaacgagccg acgtacggaa
      541 tgaacacgag gatcccggtg atgaccccga ccggaaggta gaaatcgagc ccggcgatcc
      601 acagcgcgat cgagtagtag aagctcatca cgaacatcac cgagagctgc ccgcgcagga
      661 actcggcgag cacgacgtcg gtctcccggg cgatctcgga taccttgggg tgcagggtgc
      721 ggggcacgag gctctcgacg accgcgatga gccggtccca gtcgcgcatg aggtagaaga
      781 gcacgaccgg gatgagcagc acggtgacga ggatgccgac cagcgtcgcg ccgcccactt
      841 tcagggactg gagcaccgtc gcgatcagcc cctcggtgcc cttcaccgcg tcggcaaaat
      901 agtcgcgcac gctcgccggg tcgagctgca gatcgatgcc gaagcgatcg tgcagccagg
      961 gcaccagccg cgcgttggcc tgctccagcg cgcccggcag ccgctcgccc agcatgcgcg
     1021 cctccttcgc gaacaggggc acgacgatca gcaccagcag cacgagcacg ctcgcgacca
     1081 gcgcgatgcc gatcaccgcg ccgaaggtcc ggttgacgcg gtggcgctcg agccaggtca
     1141 ccaggggatt gaggatgtac gcgaggatcc cgccgagcag gaaaggcgtg aggatcggcg
     1201 acaggaagta cagcagcagc aggaagccga gccccagcgc cagccaccac catttctctc
     1261 ggtcagcgcg gcgggcggga agcattttcg attagaattc gcgcggaaaa cgagactcta
     1321 gcggtatccc tccctcttgg ccagtcgaaa gccgatcagc taccgcgacg cgggcgtcga
     1381 tatcgacgcg ggcgacgcgc tggtcgaggc gatcaagccg ctcgcgaagc ggaccatgcg
     1441 ccccgaggtg ctcgcgggca tcggcggctt cggcgccttg tgcgagatcc cgaagcgcta
     1501 ccgcgagccc gtgctggtct cgggcaccga cggcgtcggc accaagctca agctcgcgtt
     1561 ccggctgaac cgtcacgaca cggtcggcat cgacctcgtg gcgatgagcg tgaacgacat
     1621 cctggtgcag ggcgccgagc cgctcttctt cctcgattac tacgcgtgcg gcaggctcga
     1681 cgtcggcgtc gcgacgcagg tcgtcgccgg catcgcgcgc ggctgcgatc tcgccgggtg
     1741 cgcgctgatc ggcggcgaga ccgccgagat gccgggcatg tatcccgagg gcgagtacga
     1801 tctcgccggc ttcgccgtcg gcgtcgtcga gaaaggcgcg ctcatcgacg ggcgctcgat
     1861 cgcgccgggc gacgcggtgc tcgggctggc gtcgagcggc gcgcactcga acggctactc
     1921 gctggtgcgg cgcatcctcg acgtgtcggg cgcggatccg ggatcgagct tccacgggcg
     1981 cacgctggcc gaggcgctgc tcgagcccac gcgcatctac gtcaagccgt tgctcgcgct
     2041 gatgcgcgcg atgacggtga aaggactggc gcacatcacc ggcggagggc tggtcgagaa
     2101 cgtgccgcgc gttctgcccg acggaacgaa ggccgtgctc gatcgcggcg catggacgcc
     2161 gccgccgatc ttcgcctggc tgcagcagca gggcaacgtc gccgaggccg agatgctgcg
     2221 ggtgttcaac tgcggcatcg gcatggtggt ggtggtcgac gagcgcgacg ccgcccgcgc
     2281 gcaggaggcg ctgcgcgccg ccggggagac ggcctggcgc atcggccgga tcgaggcgcg
     2341 cgcgcagggc gaggcgcagg tcgtcgtttc acgcggcgga acgtgagccg gctatttact
     2401 cgggccccgc attcgtagga tgatccctgc tttcccgggg cgggggacac cgccggccgg
     2461 ctcgcagccg gcacgaggaa tccaagacta cgacttaccg gaacgaggag ggatgatgag
     2521 aatccgcttc gcgctattca cggcgctcgc cctgctggcg ctcgtgggct gcaaccgtca
     2581 gagccaggcc ccgtacttcg aaggcaagac cctcacgatc gtcgtcggct acaagccggg
     2641 cggcggctac gacacggtgg cgcggctgct gtcgcgctac ctgccgaagt acatcccggg
     2701 cgcgccgacg gtgatcgtgc agaacatgcc cggagccaac agcctgatcg cggccaacta
     2761 cgtctacgcc caggcgaagc ccgatgggct cacgatcggc accttcaacc gcaacctgcc
     2821 gatcgggcag ctgctcaagg tcgacggcgt caagttcgac atgctgaagt tcgcgtggat
     2881 cggctcggcc gcgagcgaga ccacggtgct cgccgtccgg gatgacctgc cgtacaagac
     2941 cttcgacgac atgaggaagg cgaaggagcc gatcgtcgtc ggcgcgaccg gacccggggc
     3001 caacacctac gacttcccgc tgctgctgaa ggagttcctc ggcgtgaacc tcaagctcgt
     3061 gagcggctac tcgagcagcg ccgacatcat gctggccgtc gagcgcaagg aggccgacgc
     3121 ccgcgccggc tcgtattcgt cgatcgcgcc gttcgtcgag cgcaagctcg tgcatcccgt
     3181 catccgctcg cgcgcgagcg agcccgagat cgcgaacctg cccaccgacg agagcctcgc
     3241 gccgaacccg cgcgcgaagg cgatgatggc gctgcgctcg gcgcccgagg tcgtcggccg
     3301 cccgtacgtc atgacgcccg gcacaccgga caacctcctg aacatcctgc gcgacgcgtt
     3361 cgtgaaatcc ctcgccgacc cggacctgct cgccgaggcg aagaaatccg acatgacgct
     3421 caacttcgtc agcggcgacc aggcgctcga ggtcatgaaa gaggtcctga accagccgaa
     3481 ggacgtcgtc gacgagtttc gcaagtacgt gaagttcggc gaatgatcga gtccgccctg
     3541 gacgggctgt tgctggtcct gcagtggaag gcgctcgcct tcatggggct cggcatcctg
     3601 ctcgggttct gggtcgggct gctgccgggg ctgggcggcg tcaccacgct cgcgctcatg
     3661 ctgcccttca tctacgcgat gaagccgcac gaggcgctgg ccttcctgct cgggatgcac
     3721 tcggtggtcg ccaccacggg cgacatcacc tcggtgctgt tcggcatccc gggcgagtcg
     3781 accacggccg cgatcatcct cgacgggcat tccatggcga aaaaaggcga ggcggggcgc
     3841 gcgctcggcg ccgcgctgtc gagctcgctg ctcggcgcgc tggtcggggc gctcgcgctc
     3901 gcgctcgcga tcccgatcgt gcggccgctg gtgctggcgt tcggatctcc ggagctgttc
     3961 atgctggcca tcgtcggcct cgccttcatc gcgtcgctca gcgggcatgg cgcgcgcggg
     4021 atggtgcgcg gattcatcgc cggggggctg ggcctgctgc tcgcgacggt cgggcaggac
     4081 ccgcaggcgg gggtggcgcg cttcaccttc ggcacgctct atctctggca gggcctggac
     4141 atcgtggcgg tgctggtcgg gctgttcgcg gtgccggaga tcctcgacct ggtggtgcgc
     4201 ggcagctcga tcgcgggcga caacacgcgg gtcggcaagg tgggcagagg cgtgctcgag
     4261 ggcgtgaagg acacgttcca ccatttcggc ctggcgatcc gctgcagcct gatcgggact
     4321 ttcatcggca tcacccccgg cctgggcggt ccggtggcgc aatggatggc ctacggccac
     4381 gcggtgcaga gcgcgaagac cgaggaggag cgcaagggct tcggccgcgg cgacgtgcgc
     4441 ggcgtcatcg gcccgggcgc cgcgagcaac tcgaaggagg gcgggtcgct catcccgacg
     4501 atcgccttcg gcgtcccggg gagcgcctcg atggcggtga tcctcggcgg cttcttcctg
     4561 atgggcctcg ttcccgggcc cgacatgctg accaagaacc tggccatcac ctactcgatg
     4621 gtgtggacca tcgtgctcgc caacatcatc acggtcgcgg cgtgcctggt gttcctgaac
     4681 cggctcgcgt cgctcacgaa cgtgcccggc gagctgctcg tgccggtgat cctggtgctg
     4741 atcttcatcg gcgcgtacac cgccaacaac cagtacggcg acctcgtcgt gatgctcctg
     4801 tgcggcggga tcggctggct catggtggtc gccggctggc cgcgcgcgcc gttcgtcctc
     4861 ggcctcgtgc tcgggaagat cgccgagaac tatctctaca tctcggtcgc gcgctaccac
     4921 gcgtcgtggc tcacgcgtcc catcgtggtg gcgttgttcg cgatcgcagt cgtcgtcatc
     4981 tgctatccga tctggcaggc gcgccggacg cggagggcgc gtgcataaca aggcttcgct
     5041 cgcgctcggc gtcgtcgtca tgctcgtgtc ggcgtggggc gtgatcagcg cctggggctg
     5101 gcccgcgaag gccgcgctgt tcccgctcgt catctgtgtg ccgctgttct tcctggccgc
     5161 ggccgaggtg ctgtggtcgc tgtacggcgc gtcggctcag agcgcctccg atttcaagct
     5221 ctcggatcac ctcccgcagt cggtcgcgct gcggcgcacg gccgtcgcgg cgggatggat
     5281 cctcggcttc ttcttcggca tcctgctgct cgggttcctg atcgccgtgc cggtgttcgt
     5341 gctcgcctat ctgcgcgtcc agggcaagcg ggggtgggtg ttctccgcga tcttcacggc
     5401 ggtggtctgg gcgttcttct tcgggctgtt cgacctgctg ctgcacctgc cgtttccggc
     5461 gggctggatt ccgtcgctgc tgggcgtccg ctgagcggcc gcgcggcgtc cgctatcggc
     5521 cgcgccgcgc gaggtggatg cccgcgagca cgatcgcggc gcccgccatc tgcatccccg
     5581 acatcgcctc gccgagcagc agccacgcgt acagcgcggc gagcagcggc tgcagcagca
     5641 ggctcacggc cgagagcgac gcgggcagcc gcgcgaagcc gaacgcgatc aggctctggc
     5701 cgccgacctg cggcacgagc gcgagcgcgg ccaggaccag ccagccgcgc gcgtcctgcg
     5761 cgcggaacac ctcggcggtc gcgagcgcgt acggcaacag aacgacggcg gcgaccgcgg
     5821 tgctgaccgc catgatgcgc gcggtcgacg cgccgcgggt ggcggacttg agcgcgagca
     5881 tgtagccggc gtagaacacc gcggtgagga cgccgagcgc gtcgccggcg agcgtcctgc
     5941 cgcccagggc gaaattcggg gcgatgagga gcgccgcgcc ggccgccgtc agcccgagcg
     6001 cgccgacgaa cagcgcgctg gggcgctcgc gccacagcag ccaggccccg gcggtgacga
     6061 agaccggcgc gaaattggct tcgagcgtcg cgttcgcgac cgaggtgtag acgatcgaga
     6121 cgtgccaggc ggcgaggtcg ccggcgaagc acagtcccgc caggacgagc ggcgtccacg
     6181 atgcgggcgc cgcgcgatgc gcacccggct ccgcccgcgc ccacagcgcg agcaccggcg
     6241 ccgacagcgc gacgcgccag aacgcgctcg cccccgggct cgtgtccgag agccgcacga
     6301 ggatcggcgc gcacgcgatc atggcggc
//
LOCUS       DATDMW010000383        16255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1483710, whole genome shotgun sequence.
ACCESSION   DATDMW010000383 DATDMW010000000
VERSION     DATDMW010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483710"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VI319_16035"
     CDS             complement(<1..834)
                     /locus_tag="VI319_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865403.1"
                     /translation="MFRPAVGLALAGLALPVALLSCAGMPGASRSTMSENDGRIAGHA
                     ERMLDEGRQIFRFDTLGSEAFWGDALHLHQAIAGAANGGVGPGVSPKTALAVGLKVDA
                     DALPASLVEQIKAGKVNLDDPATTLALLKLNSVVGVTGHFDGAGKITSIGIQCAFCHA
                     TVDDSFAPGIGKRLDGWANRDLNVGAIVSLAPNLKPFTDLLGVDVATVKKVLASWGPG
                     CFDAELDKDGKALRPDGKRACTLIPPAFGLAGVNLHTWTGFGSVPYWNAYVGVTEMHG
                     SG"
     gene            1044..1421
                     /locus_tag="VI319_16040"
     CDS             1044..1421
                     /locus_tag="VI319_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865404.1"
                     /translation="MALRIHLEHRDGCAVVRVEGEWEMAGAMRTLRALIDTCRRDGIA
                     RVLIDTRDVAGNVPILDRSDIGKIMANTQGPPIAFAVVCRPERVTPERFLQVVANNRG
                     GTVKVVTEPGEGFLWLQQQRRAA"
     gene            complement(1435..2721)
                     /gene="rlmD"
                     /locus_tag="VI319_16045"
     CDS             complement(1435..2721)
                     /gene="rlmD"
                     /locus_tag="VI319_16045"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEY6865405.1"
                     /translation="MPRVTIESLDREGRGVARRDGKAVFVEGGLTGETVEYAVVRDKP
                     TFEIGRVEAVLEPSAERVEPRCRFFGICGGCAMQHLDVRAQVAAKQRVLEDALWHIGR
                     VRPETMLRAIYGEPWGYRHRARLSARFVPKKGGSLVGFHERKSSYVADMTSCEVLPGT
                     ISRLLPALRRLVDGLSIRERMPQIEVSIGDAATVLVFRVLEPPGPADGERLRAFADAH
                     GVQVWLQPAGPESAARFWPEDAPALDYALADFDVRIGFLPTDFTQVNHGVNRILVRRA
                     MALLDPRPGERIGDLFCGLGNFALPIARLGAAVTGFEGNAGLIERARANARANGLEAE
                     FRAIDLFDAAACAELPAFDKALLDPPRDGAIELVKSFAARPPRRIVYVSCDPATLARD
                     AGVLVQAQGYRFAAAGAVNMFPHPAHVESLALFERD"
     gene            complement(2735..3508)
                     /locus_tag="VI319_16050"
     CDS             complement(2735..3508)
                     /locus_tag="VI319_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY6865406.1"
                     /translation="MTPVLAFDIETVPDVAGLRLLHEIDPRVPDRDVAEFAFRERRAR
                     SGNDFLPLHLHRVIAIACSLREGETFRCWSLGDPDSSEADLIQRFFAGIDKYTPQLVS
                     WNGGGFDLPVLNYRSLIHGICAPRYWDTGDDDKDFRHNNYINRYHARHTDLMDVLSMY
                     QARATAPLDDVAQLLGFPGKLGMDGSRVWDAYQAGGIREIRNYCETDTVNTYLVFQRF
                     QMLRGACQPERYREEIELVRSTLSRYDAPHWREFLGRLK"
     gene            complement(3519..4412)
                     /gene="cysM"
                     /locus_tag="VI319_16055"
     CDS             complement(3519..4412)
                     /gene="cysM"
                     /locus_tag="VI319_16055"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEY6865407.1"
                     /translation="MSKPLTIEDTVGNTPLVRLKRLPGSTSNVVLCKLEGNNPAGSVK
                     DRPALSMIVRAEKRGAIKPGDTLIEATSGNTGIALAMAAAMRGYRMVLVMPEHLSVER
                     RQAMRAYGAEIVLTPKAGGMEAARDLAEKMRVEGKGIILDQFGNPDNPLSHYEGTGPE
                     IWRDTGGKLTHFVSSMGTTGTIMGCSRFFKEQNPKVQIIGCQPTEGSQIPGIRKWPAA
                     YLPKIYDAKRVDRIEYVSQADAEDMTRRLAREEGIFAGISSGGAVAVALRVCAEVRNA
                     VIVAIVCDRGDRYLSTGVFPD"
     gene            complement(4409..5416)
                     /gene="rfaD"
                     /locus_tag="VI319_16060"
     CDS             complement(4409..5416)
                     /gene="rfaD"
                     /locus_tag="VI319_16060"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEY6865408.1"
                     /translation="MNRYVVTGAAGFIGSNLVRALNARGETEIIAVDDLTRGDKYRNL
                     VDCSIADFLDKRDFVERLARGDFEGAVDAVFHQGACTVTTETDGRYMMENNYRYSLAL
                     LDFCLEEEVPLIYASSAAVYGASTTFREAPAFEAPLNVYGYSKLLFDQVVRRRLPEAD
                     SQVAGFRYFNVYGPREAHKQATGMVSVAWQLFRQYRADKRVRLFEGSAGYGPGEQQRD
                     FVSVEDVAKVNLHFLDHPELSGIFNLGTGKAQSFNDVAVAAINACRRAESQPPLSLAQ
                     IRDQGLIEYIPFPEKLRGKYQSYTQADLSALRRAGYRAPFLTVEEGVGRYVSALLEGE
                     R"
     gene            complement(5413..6378)
                     /gene="rfaE1"
                     /locus_tag="VI319_16065"
     CDS             complement(5413..6378)
                     /gene="rfaE1"
                     /locus_tag="VI319_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY6865409.1"
                     /translation="MNAKERPSGFDPSALGRARILVVGDAMLDRYWFGEVTRISPEAP
                     VPIVKFVRAEEQPGGAANVARNAAALGAKVSLLSVIGDDEPGGTLQQLVERDGVTACL
                     HRDGSIRTTVKLRMIGGARRQQLLRVDFETPPSREVLLDKLADYRRLLSDCEVVILSD
                     YGKGGLTHIAQMVQLARAQDKVVLVDPKGHDYSRYEGATVVTPNRDELAQVVGPWADD
                     AELADKARALRAKLKLDALLVTLGEKGMTLFQADRMLHEPAKVHEVSDVSGAGDTVIA
                     TLGVMLAIGTPLEDAMRIANSAAGVKVTKFGATVVRPDELMAALS"
     gene            complement(6375..7712)
                     /locus_tag="VI319_16070"
     CDS             complement(6375..7712)
                     /locus_tag="VI319_16070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY6865410.1"
                     /translation="MKITVNGTGYVGLVTGACLAEVGNDVLCLDIDERKIRILNEGGI
                     PIYEPGLEAVVKRNRAVGRLSFTTGIEEAVAFGTVQIIAVGTPPGEDGSADLSHVVEA
                     ARNIGRHMDGYRLVVDKSTVPVGTADQVRKAVAEELARRGAKHPFAVVSNPEFLKEGA
                     AVEDFMRPDRIVIGSDDEESTRLMRDLYAPFQRNHDRVIVMDARSAELTKYAANAMLA
                     TRISFMNEIANLAEKLGADIEHVRKGIGADPRIGYHFLYPGAGYGGSCFPKDVKALLH
                     TAAGAGGSLPILDAVEATNARQKEVLGAKIARRFGGAVRGKRVALWGLAFKPNTNDMR
                     EAPSRVLIEQLLAGGASVRAYDPIAIPEAKHLYAGRAGIDFADSPLAALDGADVLAII
                     TEWTEFRSPDFDAIKARLKTPAIFDGRNLYDPEQVARHGLEYYPIGRKVRQRA"
     gene            complement(7709..8893)
                     /gene="lapB"
                     /locus_tag="VI319_16075"
     CDS             complement(7709..8893)
                     /gene="lapB"
                     /locus_tag="VI319_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEY6865411.1"
                     /translation="MDFTDFQLWWLFGFALFFGLGWLAARIDIKHLLSESKQLPRSYF
                     RGLNFLLNEQPDKAIEAFIEVVKVDPETIELHFALGSLFRRRGETERAIRMHRNLLER
                     SDLGQEQRLHAMVELGQDYLKAGLLDRAEEVFVKLKDSTHRPAALKFLLEIYQQEKDW
                     LKAIEIARELGKDTGESWQKEIANYYCELAASEATHSRPEEAKRHLGDALAVHRKCVR
                     ANIVLGDIAASAGDHAGAIEAWKRIEQQNPVYLALVAQRLLEAYRALGRPAEGLTLLR
                     GWLSAWPSLDLLDVAFQSTLEIEGTETAYKLVKDELRRNQTLLGLDKLLEAQVLVAPV
                     ERRHDLELVRNLVHNHTRRLARYKCENCGFKARQFYWHCPACGGWETYPPKRTEETDL
                     TP"
     gene            complement(8900..9184)
                     /locus_tag="VI319_16080"
     CDS             complement(8900..9184)
                     /locus_tag="VI319_16080"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY6865412.1"
                     /translation="MKIMAWMFWLLVFAALLLFALRNTDPVTVRFYLDADWEGPLVLA
                     LLAFFALGVVCGVLACTSRLLRQRREIVGLKRELRARGADRVSAPPEAAV"
     gene            complement(9255..9542)
                     /locus_tag="VI319_16085"
     CDS             complement(9255..9542)
                     /locus_tag="VI319_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY6865413.1"
                     /translation="MTKSELIARLAARYPQLVAKDAEFAVKMILDSMTQSLVKGQRIE
                     IRGFGSFGLNHRPARTGRNPKSGEKVHVPEKFVPHFKAGKELRERVDQDQK"
     gene            complement(9570..11288)
                     /gene="rpsA"
                     /locus_tag="VI319_16090"
     CDS             complement(9570..11288)
                     /gene="rpsA"
                     /locus_tag="VI319_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY6865414.1"
                     /translation="MPNAATAVAPQQENFAAMFEESLTRKEMRTGEVITAEVIRVDVN
                     YVVVNAGLKSESFIALEEFKNDRGEVEVKPGDFTSVAIEALEDGYGETRLSRDKAKRL
                     AAWMDLEKALENGSLVKGVISGKVKGGLTVMTNGIRAFLPGSLVDIRPVKDTTPYEGK
                     EMEFKVIKLDRKRNNVVVSRRAVLEASQGAEREALLASLQEGATVKGIVKNITDYGAF
                     VDLGGIDGLLHITDLAWRRVKHPSEVLSVGDEVQAKVLKFDTEKNRVSLGLKQLGEDP
                     WVGLSRRYPPGTRLFGKVTNLTDYGAFVEVEQGIEGLVHVSEMDWTNKNVHPSKVVQL
                     GDEVEVMILEIDEERRRISLGMKQCMPNPWEEFAMNFKKGDKVKGQIKSITDFGIFIG
                     LQGGIDGLVHLSDLSWSTPGEEAVRQFKKGEEAEAVVLSIDVERERISLGIKQLEGDP
                     FNNFIAGHEKGKLVSGTVKTVDAKGAVIALAGDIEGYLRASEISRDRVEDARTHMKEG
                     DKLTAMVINVDRKNRTINLSIKQKDMAEENEAMAKLQSESAASAGSTNLGALLKAKLD
                     KQNVQQ"
     gene            complement(11383..12045)
                     /gene="cmk"
                     /locus_tag="VI319_16095"
     CDS             complement(11383..12045)
                     /gene="cmk"
                     /locus_tag="VI319_16095"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY6865415.1"
                     /translation="MPVPVIAIDGPSASGKGTVAERVARDLGYRYLDSGSLYRLVALA
                     ALRAQVPPEHAAPVAGSLDARFEGGDIWLAGERVTDAIRTEEVSAASSRVAAVPAVRA
                     ALLERQRGFRQPPGLVAEGRDMGSIVFPDASLKVFLTASPESRAQRRYKQLMDKGIAA
                     TLAGLLQDLRERDARDASRSVAPLKQGPDARLLDTTDMSAAQAAARIVEWYRGTPGGR
                     SG"
     gene            complement(12060..13337)
                     /gene="aroA"
                     /locus_tag="VI319_16100"
     CDS             complement(12060..13337)
                     /gene="aroA"
                     /locus_tag="VI319_16100"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY6865416.1"
                     /translation="MDHLDLAPVRRATGSVRLPGSKSISNRVLLLAGLARGRTEVRGL
                     LDADDTRVMRDALARLGVAVIAKEAALDVMGAGGPFPAKRAELHLGNAGTAFRPLTAV
                     LALCGGEYRLDGVARMRERPIRDLVDALNAIGARIDYTGATGYPPLAVHPAKLSLDAP
                     VRVRGDVSSQFLSALLMALPLAGRDSAVEVAGELVSKPYVEITLNVMRRFGIEARRSG
                     WSRFDVPAGVYASPGRMLVEGDASSASYFLAAGAIAGGPVRVEGVGHASIQGDVRFTD
                     VLERMGARVTVGEDWIEAASGDGLRGVDMDLNHIPDAAMTAAVVALFAEGPTTIRNVA
                     NWRVKETDRLAAMSTELRKLGARVEEGPDFIRIVPGTLTPNAAIDTYDDHRMAMSFSL
                     VALGGVPVRIMDPGCVRKTFPEYFGELARLVSA"
     gene            complement(13344..13724)
                     /locus_tag="VI319_16105"
     CDS             complement(13344..13724)
                     /locus_tag="VI319_16105"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY6865417.1"
                     /translation="MLDLDPAEHDAILASVSHLPHVLAYALVHQVARHPRSDQLFAFA
                     AGGFRDFTRIASSHPRMWTDICLANREALLAELDRYGGELARIRGLLERGDAKMLEAL
                     FAHARAARDRWLDSGQEQPRDPKS"
     gene            complement(13740..14231)
                     /locus_tag="VI319_16110"
     CDS             complement(13740..14231)
                     /locus_tag="VI319_16110"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY6865418.1"
                     /translation="MNKLVVVGVGLIGGSFALALKQAKAVGTVVGVGRSEANLRRARE
                     LGVIDAAGAFDAATLADANLVMIATPVGEMSAVFRALAPHLGRSTVVTDGGSTKQDVI
                     AAARRELGAALPRFVPGHPVAGTEKSGAGAAFADLYRGRKVILTPLAETQKGAVDLVR
                     AAW"
     gene            complement(14228..15349)
                     /gene="hisC"
                     /locus_tag="VI319_16115"
     CDS             complement(14228..15349)
                     /gene="hisC"
                     /locus_tag="VI319_16115"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY6865419.1"
                     /translation="MDLCEQCPSYIRAIAPYQPGKPISELAREMGLDEKKIIKLASNE
                     NPLGVSPKARKAIERELADLSRYPDGNAFELKATLAKRYGVSQEQLVVGNGSNDLLEL
                     VASAFLAPGRAAVYSQHAFAVYPLATIARGAAGIVVPAKSFGHDLAAMRAAITPETRV
                     VFVANPNNPTGTFVAGRELEAFVASVPQSVAVVIDEAYTDYLPPEKRYDSLSWLAKHP
                     HLIVTRTFSKVYGLAGLRVGFGVMSPKIADLLNRVRQPFNVNSLALAAATAALGDDDF
                     VKKSAAMNRDGLERIGAELKRLGIAFIPSSANFLTFRIPPIDHKPQAALVYDKLLRLG
                     VIVRPLAGYEMPDHLRVTIGTPEENERFLEALKKAMNLK"
     gene            complement(15363..>16255)
                     /gene="pheA"
                     /locus_tag="VI319_16120"
     CDS             complement(15363..>16255)
                     /gene="pheA"
                     /locus_tag="VI319_16120"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY6865420.1"
                     /translation="RAPLPAEAVGHLFGEIMSACRALEHPVSVSYLGPQGTFSEMAVV
                     KQFGASVEGLPCASIDEVVRAAETGTAQYALVPVENSTEGAIGRTLDLLLATPLKICA
                     EVVLRVQQNLIAKGTSYAKAKKVYSHAQSLAQCHGWLSQHLPGAERISVSSNAEAARL
                     AAKEAGAVAIGPEIAAERYGLKVLARSIEDDPKNRTRFLVLGAEDAGRSGADRTSLVM
                     TAHNKPGAVHDLIAPFAAHGVSMTRLESRPARTGQWEYYFYVDVEGHQGDERVARALA
                     ELRARAPFVKIFGSYPAAVL"
BASE COUNT         2396 a         5694 c         5585 g         2580 t
ORIGIN      
        1 gcccgagccg tgcatctcgg tcacgccgac gtacgcattc cagtacggca cggagccgaa
       61 gccggtccac gtatgcaggt tcacgccggc gaggccgaac gcgggcggga tcagcgtgca
      121 ggcccgcttg ccgtcgggac gcagcgcctt gccgtccttg tcgagctcgg cgtcgaaaca
      181 tcccgggccc cagctcgcca gcactttctt caccgtcgcg acgtccacgc cgagcagatc
      241 ggtgaacggc ttgagattcg gcgcgagcga gacgatggcg ccgacgttca ggtcgcggtt
      301 ggcccagccg tccaggcgct tgccgatgcc gggcgcgaag gagtcgtcga ccgtcgcgtg
      361 gcagaacgcg cactggatgc cgatcgaggt gatcttcccg gcgccgtcga agtggccggt
      421 gacgccgacc acggagttca gcttgagcag cgcgagcgtc gtcgcgggat cgtccaggtt
      481 caccttcccc gccttgatct gctccaccaa ggacgccggc agcgcatccg cgtcgacctt
      541 gaggccgacc gcgagcgcgg tcttggggct cacgcccggg ccgaccccgc cgttcgcggc
      601 accggcgatc gcctggtgca ggtgcagcgc atcgccccag aaggcctcgg agccgagggt
      661 gtcgaagcgg aagatctgcc ggccttcatc gagcatgcgc tcggcatggc cggcgatgcg
      721 gccgtcgttc tcggacatcg tgcttcggga cgcgccgggc atgccggcgc acgacagcag
      781 agcgacggga agagcgaggc cggcgagagc caagccgact gcgggtcgaa acatgggact
      841 ctccttgctg cgacgcagcg tgagtgaggg gaatggggtt gattacggag tcaagcgccc
      901 ggattagatg agactcgcgg cataacgttc ccttatgcgc gcgccgacag gcccatatcg
      961 tccgtccggc ccgaacgcgt ccggcgcgcg agggtctcac tttgccccct ccccgactcg
     1021 acgccccgaa agcgaggcga gacatggctc ttcgaattca cctggaacat cgcgacggct
     1081 gcgccgtcgt gcgggtcgaa ggcgagtggg aaatggcggg cgcgatgcgc acgttgcgcg
     1141 cgctcatcga cacctgccgc cgcgacggca tcgcgagagt gctcatcgat acgcgcgacg
     1201 tcgccggcaa cgtcccgatc ctcgaccggt ccgacatcgg caagatcatg gcgaatacgc
     1261 aggggccgcc gatcgcgttc gcggtcgtct gccggcccga gcgcgtgacg cccgagcgct
     1321 ttctccaggt cgtcgcgaac aatcgcggcg ggaccgtcaa ggtcgtgacc gagcccggcg
     1381 aaggcttcct ctggctgcag cagcagcgcc gcgccgcgtg accgcggccg ggcgctagtc
     1441 gcgctcgaac agcgcgagcg actcgacgtg cgccgggtgc gggaacatgt tcaccgcccc
     1501 ggcggcggcg aagcggtagc cctgcgcctg cacaagcacc ccggcgtcgc gcgcgagcgt
     1561 ggcgggatcg caggaaacgt agacgatgcg tcgcggcggc cgcgcggcga aggacttcac
     1621 cagctcgatc gcgccgtcgc ggggcgggtc gagcagcgcc ttgtcgaagg cgggcagctc
     1681 cgcgcacgcc gccgcgtcga acaggtcgat cgcgcggaat tccgcctcga ggccgttcgc
     1741 ccgcgcgttc gcgcgcgcgc gctcgatcag cccggcgttc ccctcgaagc ccgtcaccgc
     1801 ggcgccgagc cgcgcgatcg gcagcgcaaa attgccgagc ccgcagaaga ggtcgccgat
     1861 gcgctcgccc gggcgcggat cgagcagcgc catggcgcgc cgcacgagaa tgcggttcac
     1921 tccgtggttc acctgggtga aatcggtggg aaggaagccg atgcgaacgt cgaagtccgc
     1981 gagcgcgtag tcgagcgccg gcgcgtcctc cggccagaac cgggccgcgg actcgggccc
     2041 ggccggctgc agccagacct ggacgccgtg cgcgtcggcg aaggcgcgca gccgctcccc
     2101 gtccgcgggg ccgggcggct cgaggacgcg gaacacgagc acggtcgcgg cgtcgccgat
     2161 cgacacctcg atctgcggca tcctctcgcg gatcgacagg ccgtcgacca gtctgcgcag
     2221 cgcgggcagc agccgcgaaa tggtcccggg gaggacctcg cagctcgtca tgtccgccac
     2281 gtagctcgac ttgcgctcgt ggaagccgac gaggctgccg cccttcttcg gcacgaagcg
     2341 ggccgacagg cgcgcgcggt gccggtagcc ccacggctcg ccgtagatcg cgcgcagcat
     2401 cgtctcgggg cgcacgcggc cgatgtgcca gagcgcatcc tcgagcacgc gctgcttggc
     2461 cgcgacctgc gcgcgcacat cgaggtgctg catggcgcag ccgccgcaga tgccgaagaa
     2521 gcggcacctc ggttcgacgc gctcggcgct gggctcgagc acggcttcga ctcggccgat
     2581 ctcgaaggtc ggcttgtcgc ggacgaccgc atactccacc gtctcgccgg tcagccctcc
     2641 ctcgacgaag accgccttgc cgtcgcggcg ggcgacgccg cggccttccc ggtccaggga
     2701 ctcgatcgtc acccgcggca tgcgcgcggc cggcctactt cagccggccg aggaattcgc
     2761 gccagtgcgg cgcgtcgtac cgggacagcg tcgagcgcac gagctcgatc tcctcgcggt
     2821 agcgctccgg ctggcacgcg ccgcggagca tctggaaccg ctggaacacg agatacgtgt
     2881 tgacggtatc ggtctcgcaa tagttgcgga tctcgcggat cccgcccgcc tggtacgcat
     2941 cccacaccct ggagccgtcc atcccgagct tgcccggaaa gccgagcagc tgcgcgacgt
     3001 cgtcgagcgg cgcggtcgcg cgcgcctggt acatcgagag cacgtccatc agatccgtgt
     3061 ggcgggcgtg ataacggttg atgtagttgt tgtggcggaa atccttgtcg tcgtctcccg
     3121 tgtcccagta tcgcggagcg caaatcccgt ggatcaggct gcggtagttg agcaccggaa
     3181 gatcgaagcc gccgccgttc caggacacga gctgcggcgt gtacttgtcg atgcccgcga
     3241 aaaaacgctg gatcaaatcc gcctcgctcg aatccggatc gcccagcgac cagcaccgga
     3301 aggtctcgcc ctcgcgcagc gaacaggcga tcgcgatcac gcggtgcagg tgcagcggca
     3361 ggaaatcgtt gcccgagcgc gcgcgccgct cgcggaacgc gaactcggcg acgtcacggt
     3421 ccgggacgcg cggatcgatc tcgtgcagca gcctcaggcc cgcgacgtcc gggacggtct
     3481 cgatgtcgaa cgcgagcacc ggcgtcacgg gacgccgcct agtcggggaa cacgccggtc
     3541 gagagatagc ggtcgccgcg gtcgcagacg atggccacga tcacggcgtt cctcacctcg
     3601 gcgcacacgc gcagcgcgac cgccacggcc ccgcccgaag agatgcccgc gaagatgccc
     3661 tcctcgcgcg cgagccgacg cgtcatgtct tcggcgtcgg cctggctcac gtactcgatg
     3721 cgatcgacgc gtttcgcgtc gtagatcttc ggcaggtagg ccgcgggcca cttgcggatt
     3781 cccgggattt gcgagccctc ggtcggctgg cagccgatga tctgcacctt cgggttctgc
     3841 tccttgaaga agcgcgagca gcccatgatg gtcccggtcg tgcccatgct gctcacgaag
     3901 tgcgtgagct tgccgccggt gtcgcgccag atctccgggc ccgtgccctc gtagtgcgag
     3961 agcgggttgt ccgggttgcc gaactggtcg aggatgatgc ccttgccctc gacgcgcatc
     4021 ttctcggcca gatcgcgcgc ggcctccatg ccgccggcct tgggcgtgag cacgatctcg
     4081 gcgccgtagg cgcgcattgc ctgccggcgc tcgaccgaca ggtgctcggg catgacgagc
     4141 accatgcggt agccgcgcat cgcggccgcc atcgcgagcg cgatgccggt gttgccgctg
     4201 gtcgcctcga tcagcgtgtc gccgggcttg atcgcgccgc gcttctcggc gcgcacgatc
     4261 atcgacaggg ccggccggtc cttgaccgag ccggccgggt tgttgccttc gagcttgcac
     4321 aggacgacgt tcgacgtgct tcccgggagg cgcttcagcc gcaccagcgg cgtgttgccg
     4381 accgtgtctt cgatcgtgag cggcttgctc atcgctcgcc ctccagcagc gcgctgacgt
     4441 agcgcccgac gccctcctcg accgtgagga agggcgcgcg atagcccgcg cggcgcagcg
     4501 ccgaaagatc ggcctgggtg tagctctggt acttgcccct gagcttctcg gggaacggga
     4561 tgtattcgat cagtccctgg tcgcggatct gcgcaagcga gagcggcggc tggctctccg
     4621 cgcggcggca ggcattgatg gcggcgacgg cgacgtcgtt gaagctctgc gccttgccgg
     4681 tcccgaggtt gaagatgccc gagagctccg ggtgatcgag gaagtgcagg ttgaccttcg
     4741 ccacgtcctc caccgacacg aagtcgcgct gctgctcgcc gggaccgtag ccggccgagc
     4801 cctcgaacag gcgcacgcgc ttgtcggcgc ggtactgcct gaatagctgc cacgcgaccg
     4861 acaccatgcc ggtcgcctgc ttgtgcgcct ccctcgggcc gtagacgttg aagtagcgga
     4921 agcccgcgac ctgcgagtcg gcctcgggca ggcgccggcg caccacctgg tcgaagagga
     4981 gcttggaata gccgtacacg ttgagcggcg cctcgaacgc cggggcctcg cggaaggtcg
     5041 tgctcgcgcc gtagaccgcg gccgacgacg cgtagatgag cggcacctcc tcttcgaggc
     5101 agaagtcgag cagcgcgagc gagtagcggt agttgttctc catcatgtag cgcccgtcgg
     5161 tctcggtcgt gacggtgcac gcgccctggt ggaagaccgc gtcgaccgcg ccctcgaaat
     5221 cgccgcgtgc cagccgctcc acaaaatcgc gcttgtcgag gaaatcggcg atgctgcagt
     5281 cgaccaggtt gcggtacttg tcgccccggg tgagatcgtc gacggcgatg atctcggtct
     5341 cgccgcgggc gttcagcgcg cgcacgaggt tcgagccgat gaagccggcg gcgccggtca
     5401 cgacgtagcg gttcacgaca gcgccgccat cagctcgtcg gggcgcacca ccgtcgcgcc
     5461 gaacttggtg accttcacgc ccgccgccga gttggcgatg cgcatcgcgt cctcgagcgg
     5521 cgtgccgatt gcgagcatca cgccgagcgt cgcgatgacg gtgtcgcccg ccccgctcac
     5581 gtccgagacc tcgtgcacct tggccggctc gtgcagcatc cggtcggcct ggaacagcgt
     5641 catgcccttc tcgccgaggg tcacgagcag cgcgtcgagc ttgagcttcg cgcgcagcgc
     5701 gcgcgccttg tcggcgagct cggcgtcgtc ggcccaggga ccgacgacct gcgcgagctc
     5761 gtcgcgattg ggcgtgacga cggtcgcgcc ctcgtagcgc gagtagtcgt ggcccttggg
     5821 atcgacgagc acgaccttgt cctgcgctct tgcgagctgg accatctgcg cgatgtgcgt
     5881 cagcccgccc ttgccgtagt cggaaaggat gacgacctcg caatccgaga gaagccgccg
     5941 gtagtcggcg agcttgtcga gcagcacctc gcggctgggc ggggtctcga agtcgacgcg
     6001 cagcagctgc tgccggcgcg cgccgccgat catgcgcagc ttcacggtgg tccggatcga
     6061 gccgtcgcgg tgcaggcagg cggtgacgcc gtcgcgctcg accagctgct gcagcgtgcc
     6121 gcccggctcg tcgtcgccga tcaccgagag cagcgacacc ttggcgccga gcgcggcggc
     6181 gttgcgcgcg acgttggccg cgccacccgg ttgctcctcg gcgcgcacga atttgacgat
     6241 cggcaccggc gcctcgggcg agatgcgcgt cacctcgccg aaccagtagc ggtccagcat
     6301 cgcgtccccg acgacgagga tgcgcgcccg cccgagcgcg gacggatcga agccgctggg
     6361 tcgttccttc gcgttcatgc ccgctgcctg acctttcttc cgatcggata gtactccagc
     6421 ccgtggcgcg cgacctgctc gggatcgtag agattgcggc cgtcgaagat ggcgggcgtc
     6481 ttcagccgcg ccttgatcgc gtcgaagtcc gggctgcgga actcggtcca ctcggtgatg
     6541 atggcgagca cgtcggcgcc gtccagcgcc gcgagcggcg agtccgcgaa gtcgatgccg
     6601 gcgcgacccg cgtagagatg cttcgcctcg gggatcgcga tcgggtcgta cgcgcgcacg
     6661 ctcgccccgc cggcgagcag ctgctcgatc agcacgcggc tcggcgcctc gcgcatgtcg
     6721 ttggtgttcg gcttgaaggc gaggccccac agcgcgaccc gcttcccgcg caccgctccg
     6781 ccgaagcgcc tcgcgatctt ggcaccgagc acctctttct gccgcgcatt ggtcgcttcg
     6841 acggcatcga ggatgggcag cgagccgccc gcgccggcgg cggtgtgcag cagtgccttc
     6901 acgtccttcg ggaagcacga gccgccgtag cccgcgccgg ggtacaggaa gtgatagccg
     6961 atgcgcgggt cggcgccgat gcccttgcgc acgtgctcga tgtccgcgcc gagcttctcg
     7021 gccaggttcg cgatctcgtt catgaacgag atgcgggtcg cgagcatggc attggccgcg
     7081 tacttggtca gctccgccga gcgcgcatcc atgacgatca cgcggtcgtg attgcgctgg
     7141 aacggcgcgt agagatcgcg catcaggcgc gtcgactcct cgtcgtcgct gccgatgacg
     7201 attcggtccg gacgcatgaa gtcctcgacc gcggcgccct ccttgaggaa ctcgggattc
     7261 gagaccaccg cgaacggatg cttcgcgccg cggcgcgcca gctcctcggc gaccgccttg
     7321 cgcacctggt cggcggtgcc gacggggacg gtggacttgt cgaccacgag gcggtagccg
     7381 tccatgtgtc ggccgatgtt gcgcgcggcc tcgaccacgt gcgaaaggtc ggccgagccg
     7441 tcctcacccg gcggcgtgcc gaccgcgatg atctgcaccg tgccgaacgc gaccgcctcc
     7501 tcgatgccgg tggtgaagct caggcgcccg actgcgcggt tgcgcttgac gacggcttcc
     7561 agccccggct cgtagatcgg gatgccgcct tcgttgagga tgcggatctt gcgctcgtcg
     7621 atatcgaggc agaggacgtc gtttcccacc tcggcgaggc atgcccccgt gacgagcccg
     7681 acgtagcccg tgccgtttac agtgatcttc atggtgtcag gtcagtttcc tcggtccgct
     7741 tcggcgggta cgtctcccat ccgccgcacg cgggacagtg ccagtagaac tgccgcgcct
     7801 tgaagccgca gttctcgcat ttgtagcgcg ccaggcgccg tgtgtggttg tgcacgagat
     7861 tgcgcaccag ctccaggtcg tggcgccgct cgaccggcgc gacgagcacc tgcgcctcga
     7921 gcagcttgtc caggccgagc agcgtctggt tgcggcgcag ctcgtccttc accagcttgt
     7981 acgcggtctc ggtgccctcg atctcgagcg tcgactggaa cgcgacgtcg agcaggtcga
     8041 gcgagggcca cgccgagagc catccgcgca gaagcgtcag gccttcggcc gggcgcccga
     8101 gcgcgcggta ggcctcgagc agccgctgcg cgaccagggc gaggtagacc gggttctgct
     8161 gctcgatgcg cttccacgcc tcgatggccc cggcgtgatc ccccgcggag gccgcgatgt
     8221 cgcccaggac gatgttggcg cgcacgcact tgcggtgcac cgcgagcgcg tcgccgagat
     8281 ggcgcttcgc ctcctcgggc cgcgagtgcg tcgcctccga ggccgcgagc tcgcagtaat
     8341 aattggcgat ctccttctgc cacgactcgc ccgtgtcctt ccccagctcg cgcgcgatct
     8401 cgatcgcctt cagccagtcc ttctcctgct ggtagatctc gagcaggaac ttgagcgcgg
     8461 ccggccggtg cgtcgagtcc ttcagcttga cgaacacctc ctcggcgcgg tcgagcagcc
     8521 ccgccttgag gtaatcctgg cccagctcga ccatcgcgtg cagccgctgc tcctgcccga
     8581 ggtccgagcg ctcgagcagg ttcctgtgca tgcggatcgc gcgctcggtc tcgccgcgcc
     8641 gccggaacag gctccccagc gcgaagtgca gctcgatcgt ctcggggtcg accttcacga
     8701 cctcgatgaa cgcctcgatc gccttgtcgg gctgctcgtt caggagaaag ttcaatccgc
     8761 ggaagtagga gcgcggcagc tgcttcgact cggacagcag gtgcttgatg tcgatgcgcg
     8821 cggcaagcca gcccaggccg aagaacaggg cgaacccgaa cagccaccag agctggaaat
     8881 cggtaaagtc catcgccgct cacaccgccg cttcgggcgg cgccgaaacg cggtccgccc
     8941 cgcgcgcgcg cagctcgcgc ttgaggccga cgatctcgcg ccgctgccgc agcaggcgcg
     9001 acgtgcaggc gagcacgcca cacaccacgc cgagcgcgaa gaacgcgagc agcgccagca
     9061 ccagcggccc ctcccagtcg gcgtcgaggt agaaccgcac cgtcacggga tcggtgttgc
     9121 gcagggcgaa cagcagcagc gccgcgaaca cgagcagcca gaacatccac gccatgatct
     9181 tcattggtgc tgtctctcaa aacgcaaacg gcggcatctg caagatgccg ccgccgcgcg
     9241 atgccgaccg gcccttactt ctgatcctgg tcaacccgct cccgcagctc cttgcccgcc
     9301 ttgaagtgcg ggacgaattt ctcggggacg tggacctttt ctccggactt cgggttgcgg
     9361 cccgtccgcg ccggcctgtg gttcaggccg aagctgccga agccgcggat ctcgatccgc
     9421 tgtcccttca ccaggctctg cgtcatcgag tcgagaatca tcttgacggc gaactccgcg
     9481 tccttggcga cgagctgcgg atagcgcgcc gccagcctgg ctatcagttc cgacttcgtc
     9541 atgcgtgacc ctcccgcggg ttccgagcgc tattgctgca cgttctgttt gtcgagcttc
     9601 gccttcagca gggcacccag gttggtgctg cccgcactgg ccgcgctctc gctctgcagc
     9661 ttcgccatgg cctcgttctc ctcggccatg tccttctgct tgatcgagag gttgatcgtc
     9721 cggttcttgc ggtcgacgtt gatgaccatc gcggtgagct tgtcgccctc cttcatgtgc
     9781 gtgcgggcgt cctcgacccg gtcgcgcgag atctcggagg ctcgcaggta gccctcgatg
     9841 tcgcccgcga gcgcgatcac ggcgcccttg gcgtcgaccg tcttcaccgt gccgctcacc
     9901 agcttgccct tttcgtggcc ggcgatgaag ttgttgaacg ggtcgccctc gagctgcttg
     9961 atgccgagcg agatccgctc gcgctcgacg tcgatcgaga gcaccaccgc ctcggcctcc
    10021 tcgcccttct tgaactggcg cacggcttcc tcgcccggcg tcgaccacga caggtcggac
    10081 aggtgcacca ggccgtcgat gccgccctgc agcccgatga agatgccgaa gtcggtgatc
    10141 gacttgatct ggcccttcac cttgtcgccc ttcttgaagt tcatggcgaa ctcttcccac
    10201 gggttcggca tgcactgctt catcccgagg gagatgcggc ggcgctcctc gtcgatctcg
    10261 aggatcatga cctcgacctc gtcgccgagc tgcaccacct tggacggatg cacgttcttg
    10321 ttggtccagt ccatctcgga cacgtgcacc aggccctcga tgccctgctc gacctcgacg
    10381 aacgcaccgt aatcggtgag gttcgtgacc ttgccgaaga gacgcgtccc cgggggatag
    10441 cggcgcgaga ggccgaccca aggatcctcg ccgagctgct tcaggccgag cgacacgcgg
    10501 ttcttctcgg tgtcgaactt gagcaccttc gcctgcacct cgtcgcccac ggagagcacc
    10561 tcggaggggt gcttgacccg gcgccacgcc aggtcggtga tgtgcaggag gccgtcgatg
    10621 ccccccaggt ccacgaacgc gccgtagtcg gtgatgttct tgacgatgcc cttgaccgtc
    10681 gcgccttcct gcagcgaggc gagcagcgcc tcgcgctcgg cgccttgcga ggcctcgagc
    10741 acggcgcggc gcgacacgac cacgttgttg cgcttgcggt cgagcttgat gaccttgaac
    10801 tccatctcct tgccttcgta cggagtggtg tccttgaccg ggcggatatc gaccagcgag
    10861 ccgggaagga acgcgcggat gccgttcgtc atgacggtga ggccgccctt caccttgccg
    10921 gagatcacgc ccttcacgag cgagccgttc tcgagggctt tttccaggtc catccaggcg
    10981 gcgagacgtt tcgccttgtc gcgcgagagc cgcgtctcgc cgtagccgtc ctcgagcgcc
    11041 tcgatggcga cgctggtgaa gtcgccgggc ttgacctcga cctcgccgcg gtcgttcttg
    11101 aattcctcca gggcgatgaa gctctcggac ttcagcccgg cgttgacgac gacgtagttg
    11161 acgtcgacgc gtatcacttc cgcagtgatg acctcgcccg tgcgcatctc cttgcgcgtg
    11221 aggctttcct cgaacatcgc ggcgaagttt tcctgttggg gagccactgc ggttgctgca
    11281 ttcggcataa aagatttacc tgcacttgcc cgccgtcgcc ggcggggctg gttgataaag
    11341 acaaagacgg cccggggccg cgcttcgtcg cctaaggggt tctcacccgc tccttccgcc
    11401 aggcgtcccg cggtaccact cgacgatccg ggcggcggcc tgcgccgcgc tcatgtccgt
    11461 cgtgtcgagc agccgcgcgt ccggcccttg cttgaggggc gccacgctgc gcgacgcatc
    11521 gcgcgcgtcg cgctcgcgca aatcctgcag aaggccggca agtgtagcag cgattccctt
    11581 gtccatcaac tgtttatagc gccgctgggc ccgggattcg gggcttgccg tgaggaatac
    11641 tttgaggctg gcgtcgggaa agacgatcga ccccatgtcc cggccctcgg cgaccagtcc
    11701 tgggggctgc cggaacccgc gctggcgctc gagcagggcc gcccggacgg ccggaacggc
    11761 cgccacgcgc gaggacgccg cggaaacctc ctcggtgcgg atggcgtcgg tcacccgctc
    11821 gcccgcgagc cagatgtcgc ccccctcgaa acgcgcgtcg aggctcccgg cgacgggcgc
    11881 ggcgtgctcc gggggcacct gcgcacgcag cgcagcgagc gcgaccaggc ggtagagcga
    11941 gccgctgtcg aggtaccggt atcccaggtc ccgggccacg cgctcggcca ccgtcccctt
    12001 gcccgacgca gagggaccgt cgatggcgat caccggcacc ggcatcggaa ctgcggcgct
    12061 cacgcggaga cgagcctcgc cagctcgccg aagtactcgg gaaacgtctt gcgcacgcac
    12121 cccggatcca tgatgcgcac cggcacgccg cccagcgcga ccagcgagaa gctcatcgcc
    12181 atgcggtgat cgtcgtaggt gtcgatggcg gcgttcggcg tgagcgtacc cggcacgatg
    12241 cggatgaaat cggggccttc ctccacccgg gccccgagct tgcggagctc ggtcgacatc
    12301 gcggcgaggc gatcggtttc cttcacccgc cagttggcca cgttccggat ggtcgtcggg
    12361 ccctcggcga agagcgccac caccgcggcc gtcatcgccg catcggggat gtggttgaga
    12421 tccatgtcga cgccgcgcag gccgtctccc gaggccgcct cgatccagtc ctcgccgaca
    12481 gtcacccgcg cgcccatgcg ctcgagcacg tcggtgaagc gcacgtcgcc ctggatgctc
    12541 gcgtggccga cgccctcgac gcgcaccggc ccgcccgcga tcgcgccggc ggcgaggaag
    12601 tacgacgccg acgaggcgtc gccctcgacc agcatgcggc ccgggctcgc gtagacgccc
    12661 gcgggaacgt cgaaccggct ccagccggag cgccgcgcct cgatgccgaa gcggcgcatg
    12721 acgttcagcg tgatctcgac gtagggcttg gagaccagct cgcccgcgac ctcgaccgcc
    12781 gaatcgcggc cggcgagcgg cagcgccatg agcagcgcgc tcaggaattg cgacgagacg
    12841 tcgccgcgca cccgcaccgg cgcgtcgagc gagagcttcg cggggtgaac ggcgagcggc
    12901 ggataccccg tcgcgccggt gtagtcgatg cgcgcgccga ttgcgttcag cgcgtcgacc
    12961 aggtcgcgga tcggccgctc gcgcatgcgc gcgacgccgt cgagccggta ctcgccgccg
    13021 cacagcgcga gcacggcggt gagcgggcga aacgcggtac ccgcgttgcc caggtggagc
    13081 tcggcgcgct tcgccggaaa cggccctccc gcgcccatga cgtcgagcgc ggcttccttc
    13141 gcgatcacgg cgacgccgag gcgcgcgagc gcgtcgcgca tcacgcgcgt gtcgtcggcg
    13201 tcgagcaggc cgcggacctc ggtgcgcccg cgcgcgaggc cggcgagcag cagcacgcgg
    13261 ttggagatgc tcttcgaccc cggaagacgc acgctgcccg tcgcgcggcg gacgggggcg
    13321 aggtcgagat gatccatgcg ccctcaggac ttcgggtcgc gcggctgctc ctgcccggag
    13381 tccagccagc ggtcgcgcgc cgcgcgcgcg tgcgcgaaca gcgcctcgag catcttcgcg
    13441 tccccgcgct cgagcaggcc gcggatgcgc gcgagctcgc cgccgtagcg gtccagctcc
    13501 gcgagcaacg cctcgcggtt cgcgaggcag atgtcggtcc acatccgcgg gtggctcgac
    13561 gcgatgcgcg tgaaatcgcg gaaacccccg gccgcgaagg cgaacagctg atccgagcgc
    13621 gggtggcggg cgacctggtg caccagcgcg taggcgagca cgtgcggaag atggctgacc
    13681 gaggcgagga tcgcgtcgtg ctcggccggg tcgagatcga gcacggcggc gccgcatcgc
    13741 taccatgcgg cgcgcacgag gtcgacggcg cccttctgcg tctcggcgag cggcgtgagg
    13801 atgaccttgc ggccgcggta gagatccgcg aaggccgcgc cggcgccgct cttctcggtc
    13861 cccgcgaccg ggtgcccggg gacgaagcga ggcagcgcgg cgccgagctc gcggcgcgcc
    13921 gcggcgatca cgtcctgctt ggtgctgccg ccgtcggtga ccaccgtcga gcggccgaga
    13981 tgcggcgcga gcgcgcgaaa cacggcgctc atctcgccga ccggcgtcgc gatcatcacg
    14041 aggttcgcgt cggcgagcgt cgccgcatcg aacgcgccgg ccgcgtcgat cacgccgagc
    14101 tcgcgcgcgc gacgcaggtt cgcctcgctg cgcccgacgc cgacgaccgt gccgacggct
    14161 ttcgcctgct tgagcgcgag cgcgaaggag ccgccgatca ggccgacgcc gacaacgacg
    14221 agcttgttca tttgaggttc atcgccttct tgagcgcctc gaggaagcgc tcgttctcct
    14281 cgggcgtgcc gatcgtcacc cgcaggtggt cgggcatctc gtagcccgcg agcggccgca
    14341 cgatcacccc gaggcgcagg agcttgtcgt agaccaacgc cgcctgcggc ttgtggtcga
    14401 tcggcgggat gcggaaggtc aggaagttcg ccgacgacgg gatgaaggcg atgccgaggc
    14461 gcttcagctc ggcgccgatc cgctcgagcc cgtcgcggtt catcgccgcg cttttcttca
    14521 cgaagtcgtc gtccccgagc gcggcggtcg ccgcggcgag cgcgaggctg ttgacgttga
    14581 acggctggcg cacgcggttg agcaggtcgg cgatcttcgg gctcatgacg ccgaagccga
    14641 cacgcagccc cgcgagcccg tagaccttgg agaaggtgcg cgtgacgatc aggtgcgggt
    14701 gcttcgccag ccacgacagg ctgtcgtagc gcttctcggg cgggaggtaa tcggtgtagg
    14761 cctcgtcgat caccaccgcg acgctctgcg gcaccgacgc gacgaaggcc tcgagctcgc
    14821 gccccgcgac gaaggtgccc gtgggattgt tcgggttggc gacgaacact acgcgcgtct
    14881 cgggcgtgat cgccgcccgc atcgccgcca ggtcgtggcc gaagctcttc gcgggcacga
    14941 cgatgccggc cgccccgcgc gcgatcgtcg cgagcggata gaccgcgaac gcatgctgcg
    15001 agtagacggc cgcgcgcccg ggcgcgagga acgccgacgc gaccagctcg agcaggtcgt
    15061 tcgagccgtt gccgacgacg agctgctcct gcgacacgcc gtagcgcttc gcgagcgtcg
    15121 ccttcagctc gaacgcgttg ccgtcgggat agcgcgagag atcggcgagc tcgcgctcga
    15181 tcgccttcct cgccttgggc gacacgccga gcgggttctc gttcgaggcg agcttgatga
    15241 tcttcttctc gtcgagcccc atctcgcgcg ccagctcgga gatcggcttg cccggctggt
    15301 acggcgcgat cgcgcggatg tacgaaggac actgctcgca cagatccatg gcggcgggtc
    15361 cttcagagga cggcagcggg gtagctgccg aagatcttga cgaagggcgc cctggcgcgc
    15421 agctcggcca gcgccctggc gacgcgctcg tcgccctggt gcccctcgac atcgacgtag
    15481 aagtagtact cccactgccc ggtgcgcgcc gggcgcgact ccaggcgcgt catcgagacg
    15541 ccgtgcgcgg cgaacggcgc gatcaggtcg tgcacggcgc cgggcttgtt gtgcgcggtc
    15601 atgacgagcg aggtgcggtc cgcgcccgag cggcccgcgt cctccgcgcc gagcacgagg
    15661 aagcgcgtgc ggttcttcgg atcgtcctcg atcgagcgcg cgagcacctt gaggccgtag
    15721 cgctcggcgg cgatctcggg gccgatcgcg accgcgcccg cctccttcgc cgcgagccgt
    15781 gccgcctcgg cgttgctcga gaccgagatg cgctcggctc ccggaagatg ctgcgacagc
    15841 cagccgtggc actgggcgag cgactgcgcg tgcgagtaga ccttcttcgc cttggcgtac
    15901 gacgtgcctt tcgcgatgag attctgctgc acgcgcaaca ccacctcggc gcagatcttc
    15961 aagggggtcg cgagcagcag gtcgagcgtg cgcccgatcg cgccctcggt cgagttctcg
    16021 accggcacca gcgcgtactg cgcggtgccg gtctcggccg cgcgcaccac ctcgtcgatc
    16081 gacgcgcaag gcaggccctc gacgctcgcg ccgaactgct tcaccaccgc catctcgctg
    16141 aacgtgccct gcggcccgag gtaggacacc gagaccgggt gctcgagcgc ccggcacgcc
    16201 gacatgatct cgccgaagag atgcccgacc gcttcggccg ggagcggggc gcggg
//
LOCUS       DATDMW010000384         2776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1234753, whole genome shotgun sequence.
ACCESSION   DATDMW010000384 DATDMW010000000
VERSION     DATDMW010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234753"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VI319_16125"
     CDS             complement(<1..772)
                     /locus_tag="VI319_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY6865421.1"
                     /translation="MADRKFGIETLCLHAGQIPDAATGARAAPIYQTTSFVFDSADHA
                     ASLFNLQTFGNVYSRISNPTVAVFEERMAAVEGGRSALAAATGQAAEATAILTLCKAG
                     DHIVSASTLYGGTHTLLHVNLHKLGVETTFVSPEEPDNFRKAIRPNTKLLYAESLGNP
                     LINIVDLEALAAIAHEARVPLVIDNTVPSPYLLRPIDWGADIVLHSATKYIGGHGTTM
                     GGVIVDSGKFDWGTGKFPEFTSPSPGYHGVIFHETFGDC"
     gene            complement(811..1626)
                     /locus_tag="VI319_16130"
     CDS             complement(811..1626)
                     /locus_tag="VI319_16130"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6865422.1"
                     /translation="MRVFEGRFGRLRLCELAAGERVASENDPAVLMPQHGAELLLLNP
                     GEFHVCLGPSRMLVLHAATDWLHESFPAVFGSAEPRPFPQSRELITPRIRQLADTLAV
                     EVSNDRFLSAERLEFMLQELVLSIVETYIARRRVAAPLWRGSRFEDSRIRRAIALLRA
                     HPNKDQSIDELASQVGLSRSRFYDLFQLCTGFSPRAYLDMLCVETAIARLTSGHGKIA
                     ELAAELGFSAQSNFTRFFLNQVGVPPSEYRRAAAAGESAAPQGPAPIEYPAPK"
     gene            complement(1672..2142)
                     /locus_tag="VI319_16135"
     CDS             complement(1672..2142)
                     /locus_tag="VI319_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY6865423.1"
                     /translation="MEDINELRRILRDNRVIAVVGLSADWFRPSYFAAKYMLEHGYRV
                     IPVNPKYDEILGQKCYKSLRDVREKVDLVDVFRKTQDVMPIAEDAIAIGARVLWQQLG
                     VKNEAAAAKARAAGLDTVMDRCVKIEHGRLFGGLNWVGVNTRVISAKRPRWLPY"
     gene            2272..>2776
                     /locus_tag="VI319_16140"
     CDS             2272..>2776
                     /locus_tag="VI319_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6865424.1"
                     /translation="MTPFVARRIYRETRAVLSPEPEAVSAAKLLVAPGPVAMRMYRPA
                     GAAKDAVLPALVYFHGGGWTIGDLDTHDPLCRQLANGARCAVFSVDYRLAPEHAFPAA
                     VDDCVVATRFVAEQAEALRVDRSRLAVGGDSAGGNLAAVVALHARDAGGPALAFQLLI
                     YPATDQRC"
BASE COUNT          416 a          939 c          970 g          451 t
ORIGIN      
        1 cgcagtcgcc gaacgtctcg tggaagatca cgccgtggta gccgggcgac ggcgaggtga
       61 actcggggaa cttgccggtg ccccagtcga acttgcccga gtccacgatc acgcccccca
      121 tggtcgtgcc atgtccgccg atgtacttgg tggccgagtg cagcacgatg tccgcgcccc
      181 agtcgatcgg gcgcagcagg tagggcgagg gcacggtgtt gtcgatcacg aggggcacgc
      241 gcgcctcatg cgcgatcgcg gcgagcgcct cgaggtcgac gatgttgatg agcgggttgc
      301 cgagcgactc ggcgtacagg agcttcgtgt tcgggcggat cgccttcctg aaattgtccg
      361 gctcctcggg cgacacgaag gtggtctcga cgccgagctt gtgcaggttg acgtgcagca
      421 gggtgtgcgt gccgccgtag agggtcgagg ccgagacgat gtggtctccc gccttgcaca
      481 aggtgaggat cgcggtcgct tcggcggcct gcccggtcgc tgcggcgagc gccgagcgcc
      541 cgccttccac cgccgccatg cgctcctcga acacggcgac cgtgggattc gagatgcgcg
      601 agtagacgtt gccgaacgtc tgcaggttga aaaggctcgc ggcgtggtcg gcggaatcga
      661 acacgaagga cgtcgtctgg tagatcggtg ccgcgcgcgc gccggtcgcc gcgtccggga
      721 tctggcccgc gtgcaggcaa agcgtttcga taccgaactt gcgatccgcc atggaaaggg
      781 gcctaagggc ggattatcgc ccggccgccg ctatttcggc gccggatact cgatcggtgc
      841 ggggccctgc ggcgcggccg actcgcctgc ggccgcggca cgccggtact ccgaaggcgg
      901 cacgccgacc tggttcagga agaaccgcgt gaaattgctc tgcgccgaga aaccgagctc
      961 cgcggcgagc tcggcgatct tgccgtgccc ggaggtgagg cgcgcgatgg ccgtttcgac
     1021 gcacagcatg tcgaggtagg cgcgcggcga gaatccggtg cacagctgga agaggtcgta
     1081 gaaccgcgag cgcgaaaggc cgacctggct cgcgagctcg tcgatgctct ggtccttgtt
     1141 gggatgcgcg cgcaggagcg cgatcgcccg ccggatgcgg ctgtcctcga aacgcgagcc
     1201 gcgccacagg ggggcggcaa cgcgccgccg cgcgatatag gtctcgacga tggacagcac
     1261 cagctcctgc agcatgaact ccaggcgctc ggcggacagg aaccggtcgt tcgagacttc
     1321 gaccgcgagc gtgtcggcga gctgccggat gcgcggggtg atgagctcgc gcgactgtgg
     1381 aaaggggcgc ggctcggcgc tgccgaatac ggcggggaag ctctcgtgca gccagtcggt
     1441 cgcagcgtgc aggaccagca tccgcgacgg cccgaggcag acatgaaatt cgccggggtt
     1501 cagcaggagg agctctgcgc cgtgctgcgg catgaggacc gccgggtcgt tctccgacgc
     1561 cacccgctcg cccgcagcaa gctcgcacag ccgcagccgt ccaaagcggc cctcgaatac
     1621 gcgcatggtt cccccctatg cggacagcat agccgggcgg cggacgttgc gctagtaggg
     1681 gagccagcgc ggccgcttgg ccgagatcac ccgggtattc acgcccaccc agttcagccc
     1741 gccaaacagg cgcccgtgct cgatcttcac gcagcggtcc atcaccgtgt cgagcccggc
     1801 ggcgcgtgcc ttcgcggccg cggcctcgtt tttcaccccg agctgctgcc agagcacccg
     1861 ggcgccgatc gcgatcgcat cctccgcgat cggcatcacg tcctgggtct tgcgaaacac
     1921 gtcgacgagg tcgactttct cgcggacgtc gcgcagcgac ttgtagcact tctggccgag
     1981 gatctcgtcg tatttcgggt tgaccggtat gacgcgatag ccgtgctcca gcatgtactt
     2041 ggccgcgaag tagctcggcc ggaaccagtc ggccgaaagc ccgacgaccg caatcactcg
     2101 gttgtcgcgc aggatgcggc gcagctcgtt gatgtcctcc atgccgcgaa tactataatc
     2161 gacgcggtcg actgtgcgtt ccccgctctt tatccccagg tgagcctcca ccctcaggtc
     2221 gccgcgctgc tcgagcgcgc cgcgcactct cccttgcccg cggatcacgc ggtgacgccg
     2281 ttcgtcgccc ggcgcatcta tcgcgagacc cgcgcggtgc tctcgcccga gcccgaggcc
     2341 gtctcggcgg cgaagctgct ggtcgcgccc ggccccgtcg cgatgcgcat gtatcgcccg
     2401 gccggggcgg ccaaggacgc cgtgctcccg gcgctcgtct atttccacgg cggcggctgg
     2461 acgatcggcg atctcgacac gcacgatccg ctctgccggc agcttgccaa tggcgcgcgc
     2521 tgcgcggtgt tttccgtgga ttaccggctt gcgccggagc acgcctttcc ggcggcggtg
     2581 gacgactgcg tggttgcgac gcgcttcgtc gccgagcagg ccgaagcctt gcgcgtcgat
     2641 cgctcgcgcc tcgcggtcgg cggcgacagc gcaggcggca atctcgctgc ggtggtcgcg
     2701 ctgcacgcgc gcgacgcggg cggtcccgcg ctcgccttcc agctcctgat ctatccggcc
     2761 accgaccagc gctgca
//
LOCUS       DATDMW010000385         3619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1234817, whole genome shotgun sequence.
ACCESSION   DATDMW010000385 DATDMW010000000
VERSION     DATDMW010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234817"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VI319_16145"
     CDS             <1..285
                     /locus_tag="VI319_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY6865425.1"
                     /translation="GVVGGIMLGLGRALGETMAVTFVIGNAHKISSSLFLPGNTIAST
                     LANEFTEAVGDLYFSSLIELGLILFLITFVVLALSKLLLLRLAKREGQKT"
     gene            288..1124
                     /gene="pstA"
                     /locus_tag="VI319_16150"
     CDS             288..1124
                     /gene="pstA"
                     /locus_tag="VI319_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY6865426.1"
                     /translation="MYARRLFLNRFNLAVSLLTMLFGLFWLGWILFTLVDAGFRAIGP
                     ALFTLPTPAPGSAGGLSNAIVGSLMMAACGTLIGTPIGIMAGIHLAEFGSRGWLAPAT
                     RFINDILLSAPSIVIGLFVYEVWVVNTRHFSAWAGIMALALLVIPVVVRTTENMLRLV
                     PDSLREAAAALGAPQWKMVTMVTVRAARAGIVTGILLAVARISGETAPLLFTALNNQF
                     GSLDLNAPMANLPVVIFQFAMSPYDNWHALAWAGALLITLGVLALNIVARVFFRNRTA
                     TY"
     gene            1127..1912
                     /gene="pstB"
                     /locus_tag="VI319_16155"
     CDS             1127..1912
                     /gene="pstB"
                     /locus_tag="VI319_16155"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY6865427.1"
                     /translation="MAAVLQPAPQLQPKIQVRSLDFYYGGFHALKSISLAIPPRQVTA
                     FIGPSGCGKSTLLRTFNRMYALYPNQEARGEVLLDGENILAPGQDLNRLRARVGMVFQ
                     KPTPFPMSVHDNIAFGVRLYERLSARAMDERVEWALRQAALWDEVKDKLKQTGTSLSG
                     GQQQRLCIARAIAVRPEVLLLDEPASALDPISTGRIEELVHELKRDYTIVMVTHNMQQ
                     AARVSDFTAYMYLGEMIEFGVTDEIFIKPRRKETEDYITGRFG"
     gene            1929..2630
                     /gene="phoU"
                     /locus_tag="VI319_16160"
     CDS             1929..2630
                     /gene="phoU"
                     /locus_tag="VI319_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY6865428.1"
                     /translation="MAEHISKQYDSELETLRSGVLQMGGLVESQIRSAIEAFSTGSRE
                     LIEKVIAAEERVDQHEVDIDNICSHVIVKRQPAAGDLRLIMAIMRAVTDLERIGDEAE
                     KIARMSGQIHERGRTQLMSSGAIRHAADIAISMLRHALDALARLDARDAAKVIREDAA
                     IDDEFRSIVRQLITFMMEDPRTISTALEIVWIAKAVARIGDHAKNIAEDVIYIVKGTD
                     IRHTRFVELEGDGPR"
     gene            2641..3336
                     /gene="phoB"
                     /locus_tag="VI319_16165"
     CDS             2641..3336
                     /gene="phoB"
                     /locus_tag="VI319_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY6865429.1"
                     /translation="MGGAILVVEDEPAIQELIAVNLEHAGHRVTRALDAEQAEAEIRK
                     ALPDVVLLDWMLPGMQGLELARKLRADRRTRDIPIIMLTARAQEGDKVAGLEGGADDY
                     VTKPFSPRELLARIQAVIRRRAPQLADDAVEVDGLRLDPAAHRVKARGRNLELPPAEF
                     RLLRFLMTHPERVYTRAQLLDEIWGDHVFIEERTVDVHIRRLREALEPSGHDALVETV
                     RGTGYRFRREPAS"
     gene            complement(3333..>3619)
                     /locus_tag="VI319_16170"
     CDS             complement(3333..>3619)
                     /locus_tag="VI319_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865430.1"
                     /translation="SKLRELPKRSPEWPLVVALRLSALLHQSRRDIGLPSLECRRSAG
                     GFQVALPRAWLDRHPLTEAALGDESAQWRALGMRFSVRAASRRLPQRVAA"
BASE COUNT          563 a         1268 c         1249 g          539 t
ORIGIN      
        1 ggggtggtcg gcggcatcat gctcggcctc ggccgggcgc tcggcgagac gatggccgtc
       61 accttcgtga tcggcaacgc gcacaagatc tcctcgtcgc tgttcctccc gggcaacacc
      121 atcgcgtcca cgctcgcgaa cgagttcacc gaggcggtcg gcgacctgta cttctcatcg
      181 ctgatcgagc tggggctgat cctgttcctc atcactttcg tcgtgctcgc gctgtcgaag
      241 ctgctgctcc tgcgcctggc gaagcgcgag gggcagaaga cgtgagcctg tacgcgcggc
      301 ggctgttcct caaccgcttc aacctcgcgg tgtcgctgct gacgatgctg ttcgggctgt
      361 tctggctcgg gtggatcctc ttcacgctgg tcgatgccgg cttccgcgcg atcggcccgg
      421 cgctgttcac gctgccgacg ccggcgccgg gcagcgccgg cgggctgtcg aacgcgatcg
      481 tcggcagcct gatgatggcg gcctgcggaa ccctgatcgg cacgccgatc gggatcatgg
      541 ccggcatcca tctcgccgag ttcggcagcc gcggctggct cgcgccggcg acgcgcttca
      601 tcaacgacat cctgctgtcg gcgccctcga tcgtgatcgg cctgttcgtc tacgaggtct
      661 gggtggtgaa cacgcggcat ttctcggcgt gggcggggat catggcgctc gcgctgctcg
      721 tgatcccggt cgtcgtgcgc accaccgaga acatgctgcg cctggtgccc gacagcctgc
      781 gcgaggcggc cgctgcgctc ggggcgccgc agtggaagat ggtgacgatg gtgaccgtgc
      841 gcgccgcgcg cgcgggcatc gtcaccggga tcctgctcgc cgtggcgcgc atcagcggcg
      901 agaccgcgcc gctgctcttc accgcgctca acaaccagtt cgggtcgctc gacctgaacg
      961 cgccgatggc caacctgccg gtggtgatct tccagttcgc gatgagcccc tacgacaact
     1021 ggcacgcgct cgcttgggcg ggggcgctgc tgatcacgct gggggtgctc gcgctgaaca
     1081 tcgtcgcgcg cgtcttcttc cgcaaccgga ccgcgaccta ctgatcatgg ccgccgtgct
     1141 ccagcccgcg ccgcagctcc agcccaagat ccaggtgagg agcctcgatt tctactacgg
     1201 gggcttccat gcgctgaaga gcatcagcct cgcgatcccg ccgcgccagg tcaccgcgtt
     1261 catcggcccc tcgggctgcg gcaagtcgac gctcctgcgc accttcaacc gcatgtacgc
     1321 cctctacccg aaccaggagg cgcgcggcga ggtgctgctc gacggcgaga acatcctcgc
     1381 gccggggcag gacctgaacc ggctgcgcgc gcgcgtcggc atggtgttcc agaagccgac
     1441 gccgttcccg atgtcggttc acgacaacat cgccttcggt gtgcgtctct acgagcggct
     1501 ctcggcgcgc gcgatggacg agcgcgtcga atgggcgctg cggcaggcgg cgctctggga
     1561 cgaggtgaag gacaagctca agcagaccgg cacgagcctc tcgggcggcc agcagcagcg
     1621 cctttgcatc gcgcgcgcga tcgccgtgcg ccccgaggtg ctgctcctcg acgagcccgc
     1681 gtccgcgctc gaccccatct cgaccggccg gatcgaggag ctggtgcacg agctgaagcg
     1741 ggactacacc atcgtgatgg tgacgcacaa catgcagcag gcggcgcggg tctcggactt
     1801 caccgcctac atgtacctcg gcgagatgat cgagttcggc gtgaccgacg agatcttcat
     1861 caagccgagg cgcaaggaga ccgaggatta catcaccggc cgcttcggct gagccggaag
     1921 gaggcgccat ggcagagcac atttccaagc agtacgacag cgagctggag accctgcgct
     1981 cgggcgtgct gcagatgggc ggcctggtgg agtcgcagat ccgcagcgcg atcgaggcct
     2041 tctccaccgg cagccgcgag ctcatcgaga aagtcatcgc ggcagaggaa cgcgtcgacc
     2101 agcacgaggt cgacatcgac aacatctgca gccacgtcat cgtgaagcgc cagcccgcgg
     2161 ccggggacct gaggctcatc atggcgatca tgcgcgcggt caccgatctg gagcgcatcg
     2221 gcgacgaggc cgagaagatc gcccgcatgt cgggacagat ccacgagcgc gggaggacgc
     2281 agctcatgag ctcgggcgcg atccggcacg cggcggacat cgcgatctcg atgctgcgcc
     2341 acgcgctgga tgcgctcgcc cgtctggacg cgcgcgacgc cgcgaaggtg atccgggagg
     2401 acgccgcgat cgacgacgag ttccgctcga tcgtgcgcca gctcatcacg ttcatgatgg
     2461 aggacccgcg caccatctcc accgccctgg agatcgtctg gatcgcgaag gccgtcgcgc
     2521 gcatcggcga ccacgccaag aacatcgccg aggacgtcat ctacatcgtc aagggcaccg
     2581 acatccgcca cacgcgcttc gtcgagctcg agggcgacgg gccgcgctga ggcgcacgcc
     2641 atgggcggcg cgattctcgt cgtcgaggac gagcccgcga tccaggagct gatcgccgtc
     2701 aacctggagc acgcgggcca ccgcgtgacg cgcgcgctcg acgccgagca ggccgaggcg
     2761 gagatccgca aggcgcttcc cgacgtggtg ctgctcgact ggatgctgcc ggggatgcag
     2821 ggtctcgagc tcgcgcgcaa gctgcgcgcg gaccggcgca cgcgcgacat cccgatcatc
     2881 atgctcacgg cgcgcgcgca ggagggcgac aaggtcgcgg gactcgaggg cggcgcggac
     2941 gactacgtca ccaagccgtt ctcgccgcgc gagctgctcg cccgcatcca ggcggtgatc
     3001 cgccggcgcg cgccgcagct ggccgacgac gcggtcgagg tcgatggcct gcgcctcgac
     3061 ccggcggcgc accgcgtcaa ggcgcgcggc cgcaatctcg agctgccgcc ggccgagttc
     3121 cggctgctgc gcttcctcat gacgcacccc gagcgcgtct acacgcgcgc gcagctgctc
     3181 gacgagatct ggggcgacca cgtgttcatc gaggagcgca cggtcgacgt gcacatccgg
     3241 cgcctgcgcg aggcgctcga gccgagcggg cacgacgcgc tggtggaaac ggtgcgcggc
     3301 acggggtacc gcttccgccg cgaaccggcc tcctaagcgg cgacccgctg aggcagccgg
     3361 cgtgacgccg cgcgcaccga gaagcgcatt ccgagggcgc gccattgcgc cgactcgtcg
     3421 ccgagcgccg cttccgtgag cggatgacgg tccagccagg cgcgcggcag cgcgacctgg
     3481 aagccgccgg ccgagcgcct gcactcgagc gacggcagcc cgatgtcccg ccggctctgg
     3541 tgcagcagcg ccgacaggcg cagcgcgacc acgagcggcc attccgggct gcgcttcggc
     3601 agctcgcgca gcttcgaca
//
LOCUS       DATDMW010000386         2691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1733916, whole genome shotgun sequence.
ACCESSION   DATDMW010000386 DATDMW010000000
VERSION     DATDMW010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733916"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VI319_16175"
     CDS             complement(<1..356)
                     /locus_tag="VI319_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY6865431.1"
                     /translation="MKLCRYGRNGFEKPGVIDAQGQLRDFSSVISNIDQETISPKGLA
                     KLRKIRPESLPLVRGNPRFGVPYTGITKFVAIGLNYIDHAQEAGLQIPAEPVIFSKAT
                     TCISGPNDDIQQPRNST"
     gene            complement(371..1105)
                     /locus_tag="VI319_16180"
     CDS             complement(371..1105)
                     /locus_tag="VI319_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6865432.1"
                     /translation="MSGRLAGKVALITGAGQGMGRATALAFAREGATVWATDKNASSL
                     KDFGSEVRTRALDVTDEAAVAAAAADVGDIDVLFNCAGWVHQGTILDCSPRDWELTMN
                     VNVRSMYLVSRAFLPGMLKKKKGSIINMSSAVAAHHGVPNRFAYGTSKAAVMGLTRAI
                     AADYVRQGIRCNAVCPGTIDTPSLQDRINALADPVKARKDFIERQPMGRLGTADDITP
                     LLVYLASDESSFATGAMFSVDGGMTI"
     gene            1255..>2691
                     /locus_tag="VI319_16185"
     CDS             1255..>2691
                     /locus_tag="VI319_16185"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEY6865433.1"
                     /translation="MALSGKKKLRSREWFGREDRDGFIYRSWVQNRGVPHDQFDGRPV
                     IGICNTYSELTPCNSHFRTLAEHVKNGVLDAGGFPLEFPVMSLGETLLRPTAMLFRNL
                     ASMDVEESIRANPIDGVVLLMGCDKTTPSLLMGAASCDLPTIGVSGGPMLSGKWRGQD
                     LGSGTGVWQMSEMVRAGRLSRGDFFDAESCMSRSHGHCNTMGTASTMASMVEALGVGR
                     PGNAAIPAPDARRNVLARLAGRRIVEMVREDLVLSKLLTREAFENAIRVLAAIGGSTN
                     AVIHLIAIAGRAGVELRLDDWDRLGHELPCLVNLQPSGKYLMEDFYYAGGLPAVIREL
                     EPVLNKGALTANGRTIWENNLDAPCWNRDVIYPYDRPFKSRAGIAVLRGNLCPGGAVI
                     KPSAATPALMQHRGRAVVFEDIEDFYAKIDDEALDVDERSVMVLKNCGPKGYPGMPEV
                     GNMPLPKKLLRRGVTDMVRISDARMSGTA"
BASE COUNT          432 a          900 c          900 g          459 t
ORIGIN      
        1 gtcgagttgc gcggctgctg gatgtcgtcg ttcgggccgc tgatgcaggt cgtcgccttc
       61 gagaagatca cgggctcggc cgggatctgc aggccggcct cctgcgcgtg atcgatgtag
      121 ttcagcccga tcgcgacgaa cttggtgatc ccggtgtacg ggacgccgaa gcgcgggttg
      181 ccgcgtacga gcggcagcga ctcggggcgg atcttgcgca gcttcgcgag ccccttcggc
      241 gaaatggtct cctggtcgat gttcgagatg acgctggaaa agtcgcgcag ctgcccttgg
      301 gcatcgatga ccccgggctt ctcgaaccca ttcctgccgt aacgacaaag cttcattgtt
      361 gtcttccttt ctagattgtc attccgccgt cgaccgagaa catcgcgccg gtggcgaacg
      421 aggactcgtc ggaggcgagg tacacgagca acggggtgat gtcgtccgcg gtgccgagcc
      481 tccccatggg ctggcgctcg atgaagtcct tgcgcgcctt caccggatcc gcgagcgcgt
      541 tgatccggtc ctgcagcgaa ggcgtgtcga tggtgcccgg gcacaccgcg ttgcagcgga
      601 tgccctggcg cacgtaatcg gcggcgatgg cgcgcgtcag ccccatgacc gcggccttcg
      661 aggtgccgta ggcgaagcgg ttcgggaccc cgtgatgcgc ggcgacggcc gaggacatgt
      721 tgatgatcga gcccttcttc ttcttcagca ttcccggcag gaacgcgcgc gacacgagat
      781 acatcgagcg cacgttcacg ttcatggtga gctcccagtc cctgggcgag cagtccagga
      841 tcgtcccctg gtggacccaa cccgcgcagt tgaacaggac gtcgatgtcg cccacgtcgg
      901 ccgcagccgc cgcgacggcg gcctcgtcgg tgacgtcgag cgcccgggtg cgaacctccg
      961 agccgaaatc cttgagggag gaggcgttct tgtcggtcgc ccagaccgtc gcgccttccc
     1021 gcgcaaaggc gagcgcagtc gccctgccca tcccctggcc cgccccggtg atcagggcga
     1081 ctttcccggc cagcctaccg ctcacgacaa cccccgcttt acggtaaagc ggtcgattat
     1141 agcagtggcg tcttgcccaa gtccatggtt taacgggtaa agtacgctag agcctcgggg
     1201 aatgcatttc gttcgccctt gtctctactt aggaccctct cgacggagtc gcccatggcc
     1261 ttatcaggaa agaagaagct gcgctcccgg gaatggttcg gccgcgagga ccgcgacggc
     1321 ttcatctacc gcagctgggt ccagaaccgc ggcgttccgc acgaccagtt cgacgggcgg
     1381 ccggtcatcg gcatctgcaa cacgtactcc gagttgaccc cgtgcaattc gcatttccgc
     1441 accctcgcgg agcacgtcaa gaacggcgtc ctcgacgcgg gcggctttcc gctcgagttc
     1501 ccggtgatgt ccctcggaga aacgctgctg cgcccgaccg cgatgctgtt ccgcaacctc
     1561 gcgagcatgg acgtcgagga atcgatccgc gccaacccga tcgacggcgt cgtgctcctc
     1621 atgggctgcg acaagacgac gccctcgctg ctcatgggcg cggcctcgtg cgacctgccg
     1681 acgatcggcg tgtcgggcgg tcccatgctg tcgggtaagt ggcgcggcca ggacctcggc
     1741 tcgggcaccg gcgtctggca gatgtcggag atggtgcgcg cgggcaggct ctcgcgcggg
     1801 gatttcttcg acgccgaatc gtgcatgtcg cgctcgcacg gtcactgcaa cacgatgggc
     1861 accgcctcga ccatggcgag catggtcgag gcgctcggcg tcgggcgtcc cgggaacgcc
     1921 gcgatccccg cgcccgacgc gcggcgcaac gtgctcgcgc gcctggcggg ccggcgcatc
     1981 gtcgagatgg tgcgcgagga cctcgttctc tcgaagctgc tcacgcgcga ggcgttcgag
     2041 aacgcgatcc gcgtgctcgc ggccatcggc ggatcgacca acgcggtgat ccacctgatc
     2101 gcgatcgcgg gccgggcagg cgtcgagctg cgtctcgacg actgggaccg gctcggccac
     2161 gagctgccct gcctcgtcaa cctgcagccc tcggggaaat acctgatgga ggatttctac
     2221 tacgccggcg ggctgcccgc ggtgatccgc gagctcgagc cggtgctcaa caagggcgcg
     2281 ctcaccgcca acggccggac gatctgggag aacaacctgg acgcgccgtg ctggaaccgc
     2341 gacgtgatct atccctacga tcgcccgttc aagagccgcg ccgggatcgc cgtgctgcgc
     2401 ggcaacctct gccccggcgg cgcggtcatc aagccctcgg cagcgacgcc ggcgctgatg
     2461 cagcaccggg gccgcgccgt cgtgttcgag gacatcgagg acttctacgc gaagatcgac
     2521 gacgaggcgc tcgacgtgga cgagcgctcg gtgatggtgc tcaagaactg cgggccgaag
     2581 ggttacccgg gcatgcccga ggtcggcaac atgccgcttc cgaaaaaact cctgcgccgc
     2641 ggcgtcaccg acatggtgcg catctcggat gcgcgcatga gcggcacggc c
//
LOCUS       DATDMW010000387         2623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1860595, whole genome shotgun sequence.
ACCESSION   DATDMW010000387 DATDMW010000000
VERSION     DATDMW010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860595"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VI319_16190"
     CDS             <1..812
                     /locus_tag="VI319_16190"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6865434.1"
                     /translation="PLQPYAYSLIRVSLGAIFVPHGYAKVFGPGVVGVAKIVGAWGLP
                     APMAWGWFIGGLEFFGGILLAVGLLTRPVALMFVVEMIVAAIGVHSKIWAWNQGGAQY
                     PVFLAVYAFLIFVGGPGRYALDNRVLAGVAWAREKYADAVYALMRVALALFILPGGYN
                     KMFEGGVYRIAKGNVLKTGFAPPEFWAWVVSGVEFFGMILLLFGLFTRPVALMLAVEM
                     AVIASRVHNDAGFFWTAHGYEYALLMMGIAIAFMIGGGGRYSLDRKLGREF"
     gene            812..1774
                     /locus_tag="VI319_16195"
     CDS             812..1774
                     /locus_tag="VI319_16195"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865435.1"
                     /translation="MRKLLFVAACALACAGAFAQDNWPSRPVKVIVPSSPGGGTDLYA
                     RLLGQALSESLKQQFVIDNRPGASGNIGAEAAAKAAPDGYTMLVSANPALTVNPHLYK
                     NLPYNTERDLVPVARGVIAPQVVVVHPGVKARSIRELIDLGRREKGKLAFGSAGTGSP
                     TFLGIRLLEEITGARFTHVPYKGVGAGYKDLLGGQLAFMYPDAATAVPYVRSGKLIAL
                     AVNEKTPLLPGVPTFTELGYPQVDKVFTSFSVAVPTGTPAPIVHKLSAEIAKAMKSPA
                     LADKLEPQGLVPVFDTPEEAAKSLKQERDGWASFIHRNGLTAGD"
     gene            1849..>2623
                     /locus_tag="VI319_16200"
     CDS             1849..>2623
                     /locus_tag="VI319_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6865436.1"
                     /translation="MSAVLQQNVSAERKAFYDRIDKLNSTPLWEVLHALVTPTPATPC
                     KPYVWKWDAMWPHIMDAGRLITAKEAERRVLVLENPGLRGQSRITHSLYAGLQLILPG
                     EVAPAHRHSQSALRFILQGRGAYTAVDGEKVTMGVGDFVITPSWTFHDHGNPSKEPMV
                     WMDGLDVPIVELLDAQFMEKGPEETQHTDRDEGYNLAQFGNTMKPVQHKAKSRTSPLF
                     WYPYERTREALETLRKGQDPHPCWGHKLQYTNPATGESAM"
BASE COUNT          412 a          890 c          902 g          419 t
ORIGIN      
        1 cgccgctcca gccgtacgcc tactcgctca ttcgcgtctc gctcggcgcg atcttcgtcc
       61 cgcacggata cgccaaggtc ttcgggcccg gcgtcgtggg cgtcgcgaag atcgtcggcg
      121 cctggggcct gcccgcgccg atggcgtggg gctggttcat cggcggcctc gagttcttcg
      181 gcggcatcct gctcgccgtc gggctgctga cgcggccggt ggcgctgatg ttcgtggtcg
      241 agatgatcgt ggccgcgatc ggcgtgcact cgaagatctg ggcgtggaac cagggcggcg
      301 cccagtaccc ggtgttcctc gccgtgtacg cgttcctgat cttcgtcggc ggccccgggc
      361 gctacgcgct cgacaatcgc gtactcgccg gcgtcgcctg ggcgcgcgag aagtacgccg
      421 acgcggtcta cgcgctgatg cgcgtcgcgc tcgcgctgtt catcctgccg ggcggctaca
      481 acaagatgtt cgaaggcggc gtgtaccgca tcgccaaggg caacgtgctg aagacgggtt
      541 tcgcccctcc cgagttctgg gcctgggtgg tctcgggcgt cgagtttttc ggcatgatcc
      601 tgctcctgtt cgggctgttc acgcggccgg tcgcgctcat gctcgcggtc gaaatggccg
      661 tgatcgcgtc ccgggtgcac aacgacgccg gcttcttctg gaccgcccac ggctacgagt
      721 atgcgctgct catgatgggc atcgcgatcg cgttcatgat cggcggcggc ggacgctact
      781 cgctcgatcg caagctcgga agggagtttt gatgaggaag ctgctgttcg tcgccgcctg
      841 cgcgctcgcg tgcgcgggcg cctttgcgca ggacaactgg ccgtcgcgcc cggtcaaggt
      901 catcgtgccg tcgtcgcccg gcggcggcac cgacctgtac gcgcggctgc tggggcaggc
      961 gctctcggag tcgctcaagc agcagttcgt gatcgacaac cgtccgggcg cgagcggcaa
     1021 catcggcgcc gaagcggccg ccaaggccgc gcccgacggc tacaccatgc tggtgtcggc
     1081 gaacccggcg ctcaccgtca acccgcacct gtacaagaac ctgccgtaca acaccgagcg
     1141 cgacctcgtg ccggtcgcgc gcggggtgat cgcgccgcag gtggtggtcg tgcaccccgg
     1201 cgtcaaggcg cgctcgatcc gcgagttgat cgatctcggt cggcgcgaaa aaggcaagct
     1261 cgcgttcggc tcggcgggca cgggcagccc gacgttcctc gggatccggc tgctcgagga
     1321 gatcacggga gcgagattca cgcatgtccc gtacaagggc gtcggcgccg gctacaagga
     1381 cctgctcggc gggcagctcg cgttcatgta tccggacgcg gcgaccgcgg tgccgtacgt
     1441 gcgctcgggc aagctgatcg cgctcgcggt gaacgagaag acgccgctgt tgccgggcgt
     1501 gccgacgttc accgagctcg gctatccgca ggtcgacaag gttttcacgt cgttcagcgt
     1561 cgccgtgccg acgggcacgc cggcgccgat cgtccacaaa ctctcggccg agatcgccaa
     1621 ggccatgaag tcgcccgcgc tcgccgacaa gctcgagccc cagggcctgg tcccggtgtt
     1681 cgacacgccc gaggaggccg cgaagagcct caagcaggag cgcgacggct gggcctcttt
     1741 catccacagg aacgggctca cggccgggga ttgatcccct cgattgccaa ggcccgagcc
     1801 gaaggcggac aatagcggtc tcacgaggac acgtcccgga gatcccgcat gtccgccgtc
     1861 ctgcagcaga acgtttccgc cgagcgcaag gcgttctacg accgcatcga caagctgaac
     1921 agcaccccgc tgtgggaggt gctgcacgcg ctggtcacgc cgacgccggc cacgccgtgc
     1981 aagccgtacg tgtggaaatg ggacgcgatg tggccgcata tcatggatgc cgggcggctg
     2041 atcacggcga aggaagccga gcgtcgcgtg ctggtcctcg agaaccccgg cctgcgcggt
     2101 cagtcgcgca tcacgcacag cctgtacgcg ggactgcagc tgatcctgcc gggcgaggtc
     2161 gcgcccgcgc accggcactc gcagtcggcg ctgcgcttca tcctgcaggg ccgcggcgcg
     2221 tacaccgcgg tcgacggcga gaaggtcacg atgggcgtgg gcgacttcgt catcaccccg
     2281 tcgtggacct tccacgacca cggcaacccg tcgaaggagc cgatggtctg gatggacggg
     2341 ctcgatgtcc cgatcgtcga gctgctcgac gcgcagttca tggagaaggg accggaggag
     2401 acgcagcaca ccgaccgcga cgaaggctac aacctcgcgc agttcggcaa caccatgaag
     2461 ccggtgcagc acaaggcgaa aagccggacg tcgccgctct tctggtatcc gtacgagcgc
     2521 acgcgcgagg cgctcgagac cctgcgcaag ggccaggatc cgcacccatg ctggggccac
     2581 aagctccagt acaccaatcc cgccacgggc gagtcggcga tgc
//
LOCUS       DATDMW010000388         2345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_863139, whole genome shotgun sequence.
ACCESSION   DATDMW010000388 DATDMW010000000
VERSION     DATDMW010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863139"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="VI319_16205"
     CDS             complement(<1..1249)
                     /locus_tag="VI319_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY6865437.1"
                     /translation="MLDASDAGRFGAKAANLSALARAGLPVPPGWALGAEVYRAQLAA
                     LGLEATARGVFGSDDSATARRCALDLKLGLMEGALAPEVAHAIAAAWREVAATDRPAA
                     VRSSALVEDREGSSFAGQFQSYLGIDSEEDFAIAVRACWAALWAARALRYMGGHGIDA
                     ADTAMAVLVQPLVAARAAGGGLSRTPEGAMIVNAAPGLGAAVAQGEVAPDRYVLDRSG
                     ALRESSLGRKYHAVGCAHGRKSLARSFSANRCLDDESVAELGLLMRKCEAIVGGPVEI
                     EWALDDTGVKLLQARPLVVAEPDAESNTRDKLWLAHPRLSGHPSGVGWGEGRARVVSC
                     ECELERVAPGDVLVTTVAGPALSEVLAHVAGVVAELGGSTSHLASIARERGVPMVLGV
                     LDATQKIPDGARVAVDGVAGVVRW"
     gene            complement(1272..2312)
                     /locus_tag="VI319_16210"
     CDS             complement(1272..2312)
                     /locus_tag="VI319_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY6865438.1"
                     /translation="MTAAMHTAVLQAPRKLEVVERRQPEIGPGEVLVQTAATAVCHTD
                     LSIYTGSHPGVRYPVVMGHEATGVVADVGDGVANLKAGQHVLINPIIACGQCDQCVRG
                     AGNLCRNAGLFGREVEGSLSQYVKLPARYLHALPATMPLEAATIIETLATVRHSQTRV
                     GLAAAESVVVLGMGTAGLLHARLAVLTGASPVIAVSRTQWKLEMAERMGAHHLVSAPV
                     EGAVDEVRRLTGGKGADVVIDAAGGAETLKAAIDMLRPGGRYAAFAVSHEAVRGFSTF
                     PLYFGEISIIGSRALTPQDMNPSIQLVASGAVDVNGFITAKYPLSDAAAAFEEYERNP
                     SRILRIVIDSKA"
BASE COUNT          320 a          871 c          857 g          297 t
ORIGIN      
        1 tccagcgcac cacgccggcg acgccgtcga ccgcgacgcg cgcgccgtcg gggatcttct
       61 gcgtcgcgtc gagcacgccg agcaccatcg gcacgccgcg ctcgcgcgcg atcgacgcga
      121 ggtgcgaggt gctgccgccc aactcggcga ccacgcccgc cacgtgcgcg agcacttccg
      181 acagcgccgg ccccgcgacg gtcgtgacca gcacgtcgcc gggggcgacg cgctcgagct
      241 cgcactcgca gctcacgacg cgcgcgcggc cttcgcccca gccgacgccc gagggatggc
      301 cggagaggcg cgggtgcgcg agccagagct tgtcgcgcgt gttcgattcg gcgtcgggct
      361 ccgcaacgac gagcggccgc gcctggagca gcttcacgcc cgtgtcgtcc agcgcccact
      421 cgatctcgac cggcccgccg acgatcgcct cgcacttgcg catgaggagt ccgagctcgg
      481 cgacgctctc gtcgtcgagg cagcggttgg cggagaacga gcgcgcgagc gacttgcggc
      541 cgtgcgcgca gccgaccgcg tggtacttgc ggccgagcga cgactcgcgc agcgcgcccg
      601 agcggtcgag cacgtagcgg tcgggcgcga cctcgccctg cgcgaccgcg gcgcctaggc
      661 cgggagcggc gttcacgatc atcgcgccct cgggcgttcg cgacaggccg ccgcccgccg
      721 cgcgcgccgc gacgagcggc tgcacgagca ccgccatcgc cgtgtcggcg gcgtcgatgc
      781 cgtggccgcc catgtagcgc agcgcgcgcg ccgcccaaag cgcggcccag caggcgcgca
      841 cggcgatggc gaagtcctct tccgagtcga tgccgagata gctctggaac tggccggcga
      901 agctcgagcc ctcgcggtcc tcgaccaggg ccgaggagcg caccgccgcg ggacggtcgg
      961 tcgccgcgac ctcgcgccac gcggccgcga tcgcgtgcgc gacctcgggc gcgagcgcgc
     1021 cttccatcag gccgagcttc aggtcgagcg cgcagcgccg cgccgtggcg ctgtcgtcgg
     1081 atccgaacac gccgcgtgcc gtcgcctcga gcccgagcgc ggcgagctgc gcccggtaga
     1141 cctccgcccc gagcgcccag cccggcggaa cgggcagccc cgcgcgcgcg agcgcggaga
     1201 ggttcgccgc cttggcgccg aagcgtcctg cgtcggatgc gtccagcaac ggaacggcct
     1261 cgagcctcac gctacgcctt ggaatcgatg acgatgcgca ggatgcggct cgggttgcgc
     1321 tcgtactcct cgaacgcggc ggccgcatcg ctcaaggggt atttcgcggt gatgaagccg
     1381 ttcacgtcga ccgcgcccga ggcgacgagc tggatcgacg ggttcatgtc ctgcggcgtg
     1441 agcgcgcgcg agccgatgat gctgatctcg ccgaagtaga gcgggaaggt cgagaacccg
     1501 cgcaccgcct cgtggctgac ggcgaacgcg gcgtagcggc cgccggggcg cagcatgtcg
     1561 atcgcggcct tcagcgtctc cgcgccgccc gcggcgtcga tgaccacgtc ggcgcccttg
     1621 ccgccggtga ggcggcgcac ctcgtcgacc gcgccctcga ccggcgcgga caccaggtga
     1681 tgcgcgccca tgcgctcggc catctcgagc ttccactggg tgcgcgagac ggcgatgacc
     1741 gggctcgcgc cggtgagcac ggcgaggcgc gcgtgcagca gccccgcggt gcccatgccg
     1801 agcaccacca ccgattccgc cgcggcgagg ccgacgcggg tctgcgagtg gcgcaccgtg
     1861 gcgagcgtct cgatgatggt cgccgcctcg agcggcatcg tcgcgggcag cgcgtggaga
     1921 tagcgcgccg gcagcttcac gtactgcgag agcgagcctt cgacctcgcg gccgaagagg
     1981 ccggcgttgc ggcagagatt ccccgcgccg cgcacgcact ggtcgcactg gccgcacgcg
     2041 atgatcgggt tgatcaggac gtgctggccg gccttgagat tggcgacgcc gtcgccgacg
     2101 tccgcgacga cgccggtcgc ctcgtgcccc atgaccacgg gatagcgcac gccggggtgc
     2161 gagcccgtgt agatcgagag gtcggtgtgg cagaccgcgg tggccgcggt ctgcaccagc
     2221 acctcgccgg gcccgatctc gggttggcgg cgctcgacca cctcgagctt gcgaggcgcc
     2281 tgcagcaccg ccgtgtgcat cgctgccgtc attcgaacga catcatctcg ggaacagcgt
     2341 cgagt
//
LOCUS       DATDMW010000389         9030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_738754, whole genome shotgun sequence.
ACCESSION   DATDMW010000389 DATDMW010000000
VERSION     DATDMW010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738754"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..302)
                     /locus_tag="VI319_16215"
     CDS             complement(99..302)
                     /locus_tag="VI319_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY6865439.1"
                     /translation="MATGTVKWFNDSKGFGFITPDEGGKDLFAHHSSIQMDGYKSLKE
                     AQKVSYDVAQGAKGPAATNIRAI"
     gene            535..3069
                     /gene="mutS"
                     /locus_tag="VI319_16220"
     CDS             535..3069
                     /gene="mutS"
                     /locus_tag="VI319_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY6865440.1"
                     /translation="MSNAVEHTPMMQQYLRLKAQHPDILLFYRMGDFYELFYEDAERA
                     SRLLDITLTQRGESAGAPVKMAGVPHHSVDQYLSKLVRKGESVAICEQIGDPATSKGP
                     VDRQVTRIVTPGTLTDSSLLEDKADNILLALKREKAVAGLAWLNLASGDLRVAEVAPD
                     LLEQELRRIAPAEILVSEGVVLDVRGASAVTRLPEWHFDFAAGRKALLAQLGAATLAG
                     YGCEELEPAIAACGALLAYAKKTQGQALAHVAAVIPERAGEYVRMDAATRRNLELTET
                     LRGEPAPTLFSLLDECATGMGSRLLRHWLHHPLRDQDALRARHGAVAALGDKAADLHR
                     LLRRFADVERIAGRIALRNARPRDLSGLRDSLALLPGLAGAVPADSDLLGALARDLAV
                     PAEAHGLLARAILAEPAAIVREGGVIADGYSRELDELRALQTNSGEFLAALEARERAR
                     TAIPNLRVAYNNVHGFYIEVTHAQAQKVPADYRRRQTLKNAERYITPELKAFEDKALS
                     ARDRALALEKTLYEELLGLLAPHIGALQRIARALAQVDVLAAFAGTAAKRGYCRPELM
                     DAPGVEIEAGRHPVVEAQVEPFIANGVKLSPSRQLLLITGPNMGGKSTYMRQVALIVL
                     MAHVGSFVPARAARIGPTDQIFTRVGAADDLAGGRSTFMVEMTESANILHNATARSLV
                     LMDEVGRGTSTFDGLALAWAIAKHLLERNKALTLFATHYFEMTRLALEHKEAANVHLD
                     AVEHQDTIVFLHAVEEGPASQSYGLQVAALAGVPKGVIRSARKYLQMLEDASLTRGGQ
                     SDLFTGQGKPEVPPEPDALRETLAATNPDELSPREALDLLYRLKKL"
     gene            3080..4036
                     /locus_tag="VI319_16225"
     CDS             3080..4036
                     /locus_tag="VI319_16225"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865441.1"
                     /translation="MRRSIACLLFAAAAGASAQIPSKPLHIVVPYAAGGAVDTIARAF
                     APHLAAAFGQPVIVDDKPGAGANIGADAVAKSAPDGTTVLLSPAGLAISPAMYRRLPF
                     DAAKDFAAVSQLMSSWLILVTAQKVPAKTVSELLALAKASPGRLNYGSTGLGSPPHLA
                     GELLRQLGHIDIVHVPYKGDAPMYAALLAGEVDLGFGPLAGALPLIKAGKLHLLAITD
                     AQRSPLLPEAPTLVESGLPDFRYSGWIGVLAPGGTPRPVVERLYTEWTRALATADIRE
                     RMPAWGYEPVGSRPEEFDARFKSDLALYARIVRDAKVPRQDY"
     gene            complement(4033..4320)
                     /locus_tag="VI319_16230"
     CDS             complement(4033..4320)
                     /locus_tag="VI319_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865442.1"
                     /translation="MRLLGRWTELARRHVPLGAGCACGPGLAGVQLGDFESQVLEFLR
                     SRHGGVSCSGIGELLSGIAKGESPVPAAEREPLLGDLARTLDSFDEVHRRA"
     gene            4279..5322
                     /locus_tag="VI319_16235"
     CDS             4279..5322
                     /locus_tag="VI319_16235"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6865443.1"
                     /translation="MAARELGPAPQQAHGEDRGRSAFHRACHLIAIAAALVLAPAAFA
                     QDWPAKPIRIIVPFPAGGAVDPLVRGLSSGLAAKLGQPVLVENRPGATGTIGMAACAK
                     SPPDGYTLCFVTSDGLTVVPHLRSDLPFDAARDFAPVTLLGYPQSILVASERAPFDSF
                     AAMVAYAKRNPGKINFGTFGEGGISHQLLEAIQQGAGVKITNVPYKGTGPAIQAALAN
                     EVDLALSTFPVVAPHFPSGKLRPLAVIGRERLPALPNVPTYREQKLPIDIATWFAIMA
                     PGSVPKAIRQSTHEAIAATMAEPGFRAKVMPRDAYDAVGSGPDEFERFLVSSRAAGKK
                     IAEMLKAAGYRPE"
     gene            5319..7385
                     /locus_tag="VI319_16240"
     CDS             5319..7385
                     /locus_tag="VI319_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY6865444.1"
                     /translation="MKRGARVRLAADIGGTFTDVVAFDESARRLSLGKTLSTPARLVD
                     GISTGLELAGARYADADLFLHGSTVAINALLERTGAKTALLVTEGFRDIYEIGRINRP
                     DAYNLFFRKHVPLVERALRFEVRERLTAEGEVLVPLDEAGVEAACERLAAAGVEAVAI
                     MLLHCYVNPAHEARVKAIVQRRLPRAFVTASHELSGEYREFERCSTAAANAYIGPRVR
                     EYVRGIDAHIAAEGFAGRFLLVQSTGGLYESRQAEEQCIRMLESGPAAGVIGARALCR
                     ALGLENAVAFDMGGTTAKAGVIYRGEALTRASALVGGYAQALPVQIPMIDISEVGTGG
                     GSIAAVDATGALRVGPQSAGAEPGPACYARGGTRPTVTDANLVLGRLDPEHFLGGRMR
                     LDAAAAERALAEHVARPLGMPPARAAEGILRIAVTAMSYAVKGVSTDRGLDAAAFPLI
                     AYGGAGPLHASAIAREIGMGRVIVPRSPGHFCAFGMLHADLRYDRVRTWFMRLDDLRF
                     EEIADAFAALAAEGKAALEQSRVRAGRVAVAHAVDMRYVGQEHPVTVELPSRVLSSRD
                     REAVKRLFDAEHLRRYGTNAPGERAEIVSLRVSVSGLMKKPAPERIARGGPARPRARR
                     RVYFAEAGKAVMTPMYDRESLRAGNRIPGPALIEEEASTTVLLPGDRLRVDEFGNLDI
                     EVGRRS"
     gene            7382..>9030
                     /locus_tag="VI319_16245"
     CDS             7382..>9030
                     /locus_tag="VI319_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY6865445.1"
                     /translation="MRRAKAKADAVLTEIVRNGVIAVTEEMKTNLMRTAYNMIIYEAL
                     DFTVGLYTAEGETVSIGLGLPSFIRGMAETVKAKLRHFGPEGTGEGIHPGDILVTNDA
                     YITGSHLHHFTFTLPIFHRGKLSGFACCMAHWPDVGGLLGGVTTDIYSEGLQIPIVKY
                     RKRGVVNRDLVDIIRTNVRIPERAMGDLRAQITAITTGERRFLELLERYGREPVLDAI
                     RNIMAQSEASARARTRTIPDGTYEAESYMDDDGVEIGKRIPIKVRVVKKRDTMTIDLT
                     EVAPQVRGFFNSGMTTGYSCAQVAYKCLTSPTDYPINEGSFRPLEVKLAPGTVVTAVK
                     PAAMRWWMTFPMTVVDTLFKAMSQAVPRRVIAAHHADLLVCLINGISPKDGRFFLGGV
                     GPSGGGWGAKHDEDGVSATVCLNDGDTHNHPIEQMEAKFPLLFERHALREDSGGAGRH
                     RGGLGTEQVVRARSTIVMNVQVDRMHCPPWGLEGGLAGAGNQVTLRLGGVEKTDLPNG
                     KVLTQRLQKGDAFTLRAGGGGGFGAPFERDPERVAEDVRQG"
BASE COUNT         1326 a         3255 c         3193 g         1256 t
ORIGIN      
        1 acttccggca cgcgacggcc gcgccggcgg tcgccttccg caggccgttc cagcgccgcc
       61 gctagccggg ccccgtccgg ggcccggggc cgcgccggct atatcgcgcg gatgttggtc
      121 gccgcggggc ctttcgcgcc ctgtgcgacg tcgtaggaca ccttctgcgc ttccttgagg
      181 gacttgtagc cgtccatctg gatcgagctg tggtgcgcga agagatcctt gccgccttcg
      241 tccggcgtga tgaatccgaa gcccttcgag tcgttgaacc acttcaccgt gcctgttgcc
      301 atgttcgttc cttcttgagt gcgagacgaa atccgagccc cgcgtgcggg catgacgatc
      361 aaggaaggag ggacgaccac ttcggcagcc gcttgcgcgg caagggaggg acttcgcttc
      421 aatgcttgaa gatcactgcg cgcgcactgt agcacacatc cgcctgcgcg agttttttat
      481 tttcgggggc tcctctagaa tccggagtgc ccccgtgacg acgacaagcc ctcgatgagc
      541 aacgcggtcg agcacacccc gatgatgcag cagtacctgc gcctcaaggc gcagcatccg
      601 gacatcctgc tgttctaccg gatgggcgac ttctacgagc tgttctacga ggacgccgag
      661 cgcgcctcgc gcctgctcga catcacgctc acgcagcgcg gcgagtcggc cggcgccccg
      721 gtgaagatgg cgggcgtgcc gcaccactcc gtcgaccagt acctttcgaa gctcgtgcgc
      781 aagggcgagt cggtcgcgat ctgcgagcag atcggcgacc cggcgacctc caagggcccg
      841 gtggaccgcc aggtcacccg catcgtcacg ccggggaccc tcaccgactc gtcgctgctc
      901 gaggacaagg ccgacaacat cctgctcgcg ctgaagcgcg agaaggcggt cgccgggctc
      961 gcctggctca acctcgcgag cggcgacctg cgcgtcgccg aggtcgcgcc ggacctgctc
     1021 gagcaggagc tgcggcgcat cgcgcccgcg gagatcctgg tttccgaagg cgtcgtcctc
     1081 gacgtccgcg gcgcctccgc ggtgacgcgc ctgcccgaat ggcatttcga tttcgccgcg
     1141 ggacgcaagg cgctgctcgc gcagctgggc gcggcgacgc tcgccggcta cggctgcgag
     1201 gagctcgagc cggcgatcgc cgcctgcggc gcgctcctcg cctacgcgaa gaagacgcag
     1261 ggacaggccc tcgcccatgt cgccgcggtg atccccgagc gcgcgggcga gtacgtgcgc
     1321 atggacgccg cgacgcggcg caatctcgag ctcaccgaga ccctgcgcgg cgagcccgcg
     1381 ccgacgctgt tctcgctgct cgacgaatgc gcgaccggca tggggagccg gctgctgcgg
     1441 cactggctgc atcatccgct gcgcgaccag gacgcgctgc gcgcgcgcca cggcgcggtc
     1501 gccgcgctcg gcgacaaggc cgccgatctc caccgcctgc tgcgccgctt cgccgacgtc
     1561 gagcgcatcg ccgggcgcat cgcgctgcgc aacgcgcggc cgcgcgatct ctccgggctt
     1621 cgcgacagcc tggcgctgct gcccgggctc gccggcgccg ttccggcgga ttccgacctg
     1681 ctcggcgcgc tggcgcgcga cctcgcggtt cctgccgagg cgcacgggct gctcgcgcgc
     1741 gcgatcctgg ccgagcccgc ggcgatcgtg cgcgagggcg gcgtcatcgc cgacggctat
     1801 tcgcgcgagc tcgacgagct gcgcgcgctg cagacgaact cgggcgagtt cctcgccgcg
     1861 ctcgaggcgc gcgagcgcgc gcgcaccgcg attccgaacc tgcgcgtcgc gtacaacaac
     1921 gtgcacggct tctacatcga ggtcacccat gcccaggcgc agaaggtgcc cgcggactac
     1981 cgccgccgcc agacgttgaa gaacgccgag cgctacatca cgcccgagct gaaagccttc
     2041 gaggacaagg cgctgtcggc gcgcgaccgc gcgctcgcgc tcgagaagac gctgtacgag
     2101 gagctgctcg ggctgctcgc cccgcacatc ggggcgctgc agcggatcgc gcgcgcgctc
     2161 gcgcaggtcg acgtgctcgc ggcgttcgcc gggaccgcgg cgaagcgcgg gtattgccgt
     2221 cccgagctga tggacgcgcc gggggtcgag atcgaggcgg gacggcatcc ggtcgtcgag
     2281 gcccaggtcg agccgttcat cgcgaacggc gtgaagctct ccccttcgcg ccagctgctg
     2341 ctcatcaccg gcccgaacat gggcggcaag tcgacgtaca tgcggcaggt ggcgctgatc
     2401 gtgctgatgg cgcacgtcgg atcgttcgtc ccggcgcgag cggcccgcat cggaccgacg
     2461 gaccagatct tcacgcgcgt cggcgccgcg gacgacctcg ccggcggccg ctcgaccttc
     2521 atggtcgaga tgaccgaatc ggccaacatc ctgcacaacg cgacggcgcg cagcctcgtg
     2581 ctcatggacg aggtcggccg cggcacctcg accttcgacg gcctggcgct cgcctgggcg
     2641 atcgcgaagc acctgctcga gcgcaacaag gcgctgacgc tgttcgcgac gcactacttc
     2701 gagatgacgc ggctcgcgct cgagcacaag gaggcggcga acgtgcacct cgacgcggtg
     2761 gagcaccagg acacgatcgt gttcctccac gcggtggagg aagggccggc gtcgcagagc
     2821 tacgggctgc aggtcgccgc gctcgcgggc gtgccgaagg gcgtgatccg cagcgcgcga
     2881 aaatacctgc agatgctcga ggacgcgagc ctgacgcgcg gcggacaatc ggacctgttc
     2941 acgggccagg gcaagcctga ggtcccgccc gagcccgacg ccctgcgcga gacgctcgcc
     3001 gcgaccaacc ccgacgagct ctcgccgcgc gaggcgctcg acctgctgta ccggttgaag
     3061 aaactgtgac gagggcatca tgagacgatc gatcgcgtgt ctccttttcg ccgccgcggc
     3121 gggcgcttcg gcgcagatcc cgagcaagcc gctccacatc gtcgttccct acgccgccgg
     3181 cggcgcggtc gacaccatcg cgcgcgcctt cgcgccgcac ctcgcggccg cgttcggcca
     3241 gccggtgatc gtcgacgaca agccgggcgc cggcgcgaac atcggcgcgg acgcggtggc
     3301 gaagtcggcg cccgacggca cgacggtgct gctctcgccc gcggggctcg cgatcagccc
     3361 cgccatgtac cggaggctcc cgttcgacgc ggcgaaggac ttcgccgccg tgtcgcagct
     3421 catgtcgtcg tggctgatcc tggtcaccgc gcagaaggtc ccggcgaaga ccgtgagcga
     3481 gctgctcgcg ctcgcgaagg cgagcccggg ccgcctcaac tacggttcga ccggcctcgg
     3541 cagcccgcct cacctggcgg gggagctgct gcggcagctc ggccacatcg acatcgtgca
     3601 cgtcccgtac aagggcgacg ccccgatgta cgcggcgctc ctcgccggcg aggtcgacct
     3661 cggtttcggc ccgctcgcgg gcgcgctgcc cctgatcaag gccgggaagc tgcacctgct
     3721 cgcgatcacc gacgcgcagc gctcgccgct gctgcccgag gcgccgacgc tggtcgagtc
     3781 gggcctgccc gatttccgct actcgggctg gatcggcgtg ctcgcgcccg ggggcacgcc
     3841 gcgcccggtc gtcgagcgac tgtacacgga gtggacgcgc gcgctcgcca ccgccgacat
     3901 ccgcgagcgc atgccggcct ggggctacga gccggtcggc agccgccccg aggaattcga
     3961 cgcccggttc aagtcggacc tcgcgctcta cgcgcgcatc gtgcgcgacg cgaaggtccc
     4021 gcggcaggac tactaggcgc gccgatgcac ctcgtcgaac gagtcgagcg tacgcgcgag
     4081 atcgccgagc agcggctcgc gctcggccgc ggggaccggg ctctcgccct tcgcgatccc
     4141 cgacagcagc tccccgattc cggaacagga gaccccgccg tgacgcgagc gcaggaactc
     4201 gagcacctgg gattcgaaat cgccgagctg cacgcccgcg aggcccggcc cgcaggcgca
     4261 gcccgcgccg agcgggacgt ggcggcgcgc gagctcggtc cagcgcccca gcaggcgcac
     4321 ggcgaggatc gaggacgaag cgcttttcat cgcgcgtgcc atcttatcgc gatcgccgcg
     4381 gcactggtcc tcgcgcccgc ggcgttcgcg caggactggc ccgcgaagcc gatccgcatc
     4441 atcgtcccct tccccgcggg cggcgcggtc gatccgctgg tgcgcggcct ctcgagcggg
     4501 ctcgcggcga agctcgggca gccggtgctg gtcgagaacc gccccggcgc gaccggcacg
     4561 atcggcatgg cggcgtgcgc gaaatcgccg cccgacggct atacgctctg cttcgtcacg
     4621 agcgacggcc tcacggtcgt tccccatctg cgctcggacc tgcctttcga cgccgcgcgc
     4681 gacttcgcgc cggtgacgct gctcggctat ccgcagtcga tcctggtcgc gagcgagcgc
     4741 gcgcccttcg attccttcgc cgcgatggtc gcgtatgcga agcgcaaccc gggaaagatc
     4801 aatttcggca ccttcggcga gggcggcatc tcgcaccagc tgctcgaggc gatccagcag
     4861 ggcgcgggcg tgaagatcac caatgttccg tacaaaggca ccggcccggc gatccaggcg
     4921 gcgctcgcga acgaggtcga cctcgcgctc agcaccttcc cggtggtcgc gccgcacttt
     4981 ccgtccggca agctgcggcc actcgccgtg atcggccgcg agcgcctgcc cgcgctcccg
     5041 aacgtcccga cctaccgcga gcagaagctg ccgatcgaca tcgcgacctg gttcgcgatc
     5101 atggcgcccg ggagcgtgcc gaaggcgatc cggcagagca cgcacgaggc gatcgccgcg
     5161 acgatggccg agcccgggtt ccgcgcgaag gtgatgccgc gcgacgcgta cgatgccgtc
     5221 gggagcggcc ccgacgagtt cgagcgcttt ttggtttcga gccgcgccgc gggaaagaag
     5281 atcgccgaga tgctcaaggc ggcgggctac cgtccggaat gaagcgcggc gcccgggtcc
     5341 gcctcgccgc cgacatcggc ggcaccttca ccgacgtcgt cgccttcgac gaaagcgcga
     5401 ggcgcctttc tctcgggaag acgttgtcga ccccggcccg gctggtcgac gggatctcga
     5461 ccggcctcga gctcgccggc gcgcgctacg ccgatgccga tcttttcctg catggatcga
     5521 ccgtcgcgat caacgcgctg ctcgagcgca ccggcgcgaa gacggcgttg ctcgtcaccg
     5581 agggcttccg cgacatctac gagatcggcc gcatcaaccg gcccgacgcc tacaacctgt
     5641 tcttccgcaa gcacgtgccc ctggtcgagc gcgcgctgcg cttcgaggtg cgcgagcgcc
     5701 tcaccgccga gggcgaggtc ctggtgccgc tcgacgaggc gggcgtcgag gccgcttgcg
     5761 agcgcctcgc ggcggccggc gtggaagcgg tcgcgatcat gctgctccat tgctacgtca
     5821 accccgcgca cgaggcgcgg gtgaaggcga tcgtgcagcg gcggctgccg cgcgcgttcg
     5881 tcaccgcctc gcacgagctc tcgggcgagt accgcgagtt cgagcgctgc tcgaccgccg
     5941 ccgccaacgc ctacatcggc ccgcgcgtgc gcgagtacgt gcgcggcatc gacgcgcaca
     6001 tcgccgcgga aggcttcgcg ggccggttcc tgctcgtgca gtcgaccggc gggctgtacg
     6061 agtcgcggca ggccgaggag cagtgcatcc gcatgctcga gtccggcccg gcggcgggcg
     6121 tgatcggcgc gcgtgcgctg tgccgcgcgc tcggcctcga gaacgcggtc gcgttcgaca
     6181 tgggcggcac gaccgcgaag gcgggcgtga tttaccgcgg cgaggcgctg acgcgcgcga
     6241 gcgcgctcgt cggcggctac gcgcaggcgc tgccggtgca gatcccgatg atcgacatct
     6301 cggaggtggg aacgggcggc ggcagcatcg ccgccgtcga cgcgaccggc gcgctgcgcg
     6361 tcggcccgca gagcgcgggc gccgagcccg ggcccgcgtg ctacgcgcgc ggcggcacgc
     6421 ggccgacggt gaccgacgcc aacctcgtgc tcgggcgtct ggacccggag cacttcctcg
     6481 gcgggcgcat gcggctcgac gccgcggccg ccgagcgggc gctcgccgag cacgtcgcgc
     6541 gcccgctcgg gatgccgccc gcgcgcgccg ccgaaggcat cctgcgcatc gcggtgacgg
     6601 cgatgtcgta cgcggtgaag ggcgtctcga ccgaccgcgg cctcgacgcg gccgcgttcc
     6661 cgctcatcgc gtacggcggc gcggggccgc tccatgcgag cgcgatcgcg cgcgagatcg
     6721 gcatggggcg cgtgatcgtc ccgcgctcgc ccggccattt ctgcgccttc ggcatgctgc
     6781 acgcggacct ccgctacgac cgggtgcgga cctggttcat gcggctcgac gacctgcgct
     6841 tcgaggagat cgcggacgcg ttcgccgcgc tcgccgccga gggcaaggcc gcgctcgagc
     6901 agagccgcgt gcgtgccggc cgcgtcgccg tcgcgcacgc ggtcgacatg cgctacgtgg
     6961 ggcaggagca cccggtgacg gtcgagcttc cctcgcgggt gctgtcttcg cgagaccggg
     7021 aagcggtgaa aaggctgttc gacgccgagc acctgcgccg ctacggcacg aacgcgcccg
     7081 gcgagcgcgc cgagatcgtg agcctgcgcg tctcggtctc gggcctgatg aagaagcccg
     7141 cgcccgagcg catcgcgcgc ggcggcccgg cgcgcccgcg cgcgagaagg cgggtctact
     7201 tcgccgaagc cggaaaggcg gtcatgacgc cgatgtacga ccgggagtcg ctgcgcgccg
     7261 ggaaccgcat ccccggtccc gcgctcatcg aggaggaagc gtcgacgacc gtcctgcttc
     7321 cgggcgaccg gctgcgcgtc gacgagttcg gcaatctcga tatcgaagtg gggcgaaggt
     7381 cgtgagacgc gccaaagcga aagccgacgc ggtcctgacc gagatcgtgc gcaacggcgt
     7441 catcgcggtg accgaggaga tgaagaccaa cctcatgcgc accgcgtaca acatgatcat
     7501 ctacgaggcg ctcgacttca ccgtcggcct gtatacggcc gagggcgaga cggtgtcgat
     7561 cgggctcggg ctgccgagct tcatccgcgg gatggccgag acggtgaagg cgaagctgcg
     7621 ccacttcggg cccgaaggca ccggcgaggg catccacccg ggcgacatcc tcgtcacgaa
     7681 cgacgcgtac atcaccggct cgcacctgca ccacttcacc ttcacgctgc cgatcttcca
     7741 ccgcgggaag ctctcgggct tcgcgtgctg catggcgcac tggcccgacg tcggcgggct
     7801 gctcggcggc gtgacgacgg acatctactc cgagggcctg cagatcccga tcgtgaagta
     7861 ccgcaagcgc ggcgtcgtga accgcgacct ggtcgacatc atccgcacca acgtgcgcat
     7921 ccccgagcgc gcgatgggcg acctgcgggc gcagatcacc gcgatcacca ccggcgagcg
     7981 gcgcttcctc gagctgctcg agcgctacgg gcgcgagccg gtgctcgacg cgatccgcaa
     8041 catcatggcg cagtcggagg cctcggcgcg cgcgcgcacg cgcacgatcc ccgacggcac
     8101 gtacgaggcc gagtcgtaca tggacgacga cggcgtcgag atcggcaagc gcatcccgat
     8161 caaggtgcgc gtggtgaaga agcgcgacac gatgacgatc gatctcaccg aggtcgcccc
     8221 gcaggtgcgc ggcttcttca actcggggat gacgaccggc tactcgtgcg cgcaggtcgc
     8281 gtacaagtgc ctcacctcgc ccaccgacta cccgatcaac gagggcagct tccggccgct
     8341 cgaggtgaag ctcgcgccgg gcacggtcgt gaccgcggtc aagcccgcgg cgatgcgctg
     8401 gtggatgacg ttcccgatga cggtggtcga cacgctgttc aaggcgatgt cgcaggcggt
     8461 gccgcggcgc gtgatcgccg cgcaccacgc cgacctgctc gtgtgcctga tcaacggcat
     8521 ctcgccgaag gacgggcgct tcttcctcgg cggcgtcgga ccctcgggcg gcggctgggg
     8581 cgcgaagcac gacgaggacg gcgtcagcgc gaccgtgtgc ctgaacgacg gcgacacgca
     8641 caatcacccg atcgagcaga tggaggcgaa gttcccgctg ctgttcgagc ggcatgccct
     8701 gcgcgaggac tcgggcggcg cgggccgcca ccgcggcggc ctcggcaccg agcaggtcgt
     8761 gcgcgcgcgc tcgaccatcg tgatgaacgt gcaggtcgac cgcatgcact gcccgccgtg
     8821 gggcctggag ggcgggctcg cgggcgcggg caaccaggtg acgctgcgcc tgggcggcgt
     8881 cgagaagacc gatctgccga acggcaaggt gctgacgcaa aggctccaga aaggcgacgc
     8941 cttcaccctg cgcgcgggcg gcggcggcgg gttcggagcg ccgttcgagc gcgatccgga
     9001 gcgggtcgcc gaggacgtgc gccagggcta
//
LOCUS       DATDMW010000390        14297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_2484724, whole genome shotgun sequence.
ACCESSION   DATDMW010000390 DATDMW010000000
VERSION     DATDMW010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484724"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VI319_16250"
     CDS             complement(<1..238)
                     /locus_tag="VI319_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY6865446.1"
                     /translation="MATTLADVRKTAEQKMQKSLDALKADLAKVRTGRAHAGILDHVM
                     VDYYGSAMPIPKVANVALVDARTIGITPWEKKMVH"
     gene            complement(251..970)
                     /gene="pyrH"
                     /locus_tag="VI319_16255"
     CDS             complement(251..970)
                     /gene="pyrH"
                     /locus_tag="VI319_16255"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY6865447.1"
                     /translation="MPEQAKFKRVLLKLSGEALMGEDAYGINRATIERIVSEIGQVAA
                     LGLELGVVIGGGNIFRGVAPGAQGMDRATADYIGMMATIMNALALQDAMRQAGLNARV
                     QSALNIEAVVEPYIRNKAIRYLEEGKIVIFAAGTGNPFFTTDTAAALRGTEIGAEMVL
                     KATKVDGVYTADPKRHPDAKRYDKVSFDEAMIKHLEVMDATAFALCRDQKLPITVFSI
                     YKPGAMKRIVTGEKEGTLVYY"
     gene            complement(975..1868)
                     /gene="tsf"
                     /locus_tag="VI319_16260"
     CDS             complement(975..1868)
                     /gene="tsf"
                     /locus_tag="VI319_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY6865448.1"
                     /translation="MAEITAGMVRALRDKTDAPMMECKKALAEASGDLDKAEEILRIK
                     LGNKASKAASRVAAEGIVAIALSGDAKAGAIVEVNCETDFVAKNDDFIALANGLAKLV
                     LEKKPADVAALSALPYQGSTVEEVRKALVGKIGENLSVRRFARLDAKGRIAQYVHGGS
                     KIGVMVDVTGGDEQLAKDLSMHIAAAKPLALSKDQVPADLVKKERDIATAKAIEDGKP
                     ADLVPKIAEGAVQKFFKTVTLLGQGFVKDEKQSIEALLKSKGAAVGGFVLYVVGEGIE
                     KKSSDFAAEVAAQAKQAEKAH"
     gene            complement(1948..2700)
                     /gene="rpsB"
                     /locus_tag="VI319_16265"
     CDS             complement(1948..2700)
                     /gene="rpsB"
                     /locus_tag="VI319_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY6865449.1"
                     /translation="MSTTMRQMLEAGVHFGHQTRYWNPKMAPFIFGHRNKIHIINLEK
                     TMVQFQEAMKFVRQLSSNKGTILFVGTKRQAREIMREEAERAGMPYVDHRWLGGMLTN
                     FKTIKQSIKRLKEMDAAVQDGSVDRMSKKEALHFQREMEKLQRSLGGIKQLDSLPDAL
                     FVVDVGYQKGAVAEAKKLGVPVIGVVDTNHSPDGVAYVIPGNDDSSRAIRLYARGVAD
                     AVLEGRSQSLQEIVGATSDEFVEVEEAEGKAG"
     gene            2935..3732
                     /gene="map"
                     /locus_tag="VI319_16270"
     CDS             2935..3732
                     /gene="map"
                     /locus_tag="VI319_16270"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY6865450.1"
                     /translation="MAIQLKTTEDIQKMRVAGRLAADVLDFITPHVRPGITTGELDRL
                     CHEYMVDEQKTVPAPLNYAPPGYKPFPKAICASVNHQVCHGVPGDRVLKAGDILNLDI
                     TVIKDGWHGDTSRMFYVGEPSIQARRLCEVTYECMWLGIRSVRPGAHLGDIGSAIQHN
                     AESHGYSVVREFCGHGIGRKFHEEPQVLHYGRAGAGVRLEPGMIFTIEPMINAGKAAI
                     RELADGWTIVTKDHSLSAQWEHTVLVTPEGYEVLTLSPGSPPPPPPS"
     gene            3729..6317
                     /locus_tag="VI319_16275"
     CDS             3729..6317
                     /locus_tag="VI319_16275"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY6865451.1"
                     /translation="MTALAPAVRPAPRVDLAALKGNLATGRAALRERFEASGDPAALL
                     RGHCRLVDGVLRDLWTRLDMPAGFALLAVGGYGRGELFPHSDVDILVLLPGDPSPGDA
                     AKVEEFIGVLWDSGLETGHSVRTVRDCVEEAAKDITVRTSMLEARRLAGGAGLFRDFE
                     DAARAALQTPVFFKAKRLEQEQRHTKYADSPYSLEPNVKEAPGGLRDLQVILWISRAA
                     ALGRDWSELARRGLITTAEARQAKRDQRFLQQVRVRLHYATDRREDRLLFDYQEAVAR
                     SMGIAATAHRRASEELMQRYFRTAKTVTQLNTILLQNLGAEIFPSRESDVEIIDERFR
                     RVGELLDARGEDLFARHPDAILESFLVMQKRSELKGMTAQTLRALWRARDRIDARFRA
                     DPRNRATFLELLQQPRGIVHEFRRMNQYGILGRYLPAFRRIVGQMQHDLFHVYTVDQH
                     ILQVVRNVRRFTMLEFAHEYPLCSRLIAGFERHWLLYVAALFHDIAKGRGGDHSKLGK
                     TEARRFCRDHGIAGEDAELVEFLVESHLTMSSVAQKQDLGDPDTVRAFADVVKTERRL
                     VALYLLTVADIRGTSPKVWNAWKGKLLEDLFRLTQRLLRGGSFEADQYIHDKQEEAKR
                     LLRLYALSDTVQDALWKSLDVPYFLRHDAQEIAWHTRTLHYRVDGDKPVVKARLSPAG
                     EGLQVMIYVVDQPNLFARICGYFGSIQFNIADAKIYTTRNGYALDTFQVMDTGSGAHY
                     RDMIALIESELADWLAREHELPAPITGRVSRRVKHFPIAPEVHIVPDERAHYHSLAVT
                     AGDRPGLLYAVARVLGKYRIDLHTAKIVTLGERAEDVFVVSGSALSNPKTVLQLETDL
                     LEALQS"
     gene            complement(6318..6848)
                     /gene="def"
                     /locus_tag="VI319_16280"
     CDS             complement(6318..6848)
                     /gene="def"
                     /locus_tag="VI319_16280"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY6865452.1"
                     /translation="MIRPVLRMGDPRLLERSREVEAFGTAELRELIADMRETMAHLNG
                     AGLAAPQIGVPQRVVIFGVKANPRYPDAEEVPDTVLINPVVKPVGREMEEGWEGCLSV
                     PGMRGVVPRYRTIRYTGFDALGEPIDRTVEGFHARVVQHECDHLDGILYPMRIQDFSQ
                     FGFVDVLFPGQDVVDE"
     gene            complement(6845..7579)
                     /locus_tag="VI319_16285"
     CDS             complement(6845..7579)
                     /locus_tag="VI319_16285"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEY6865453.1"
                     /translation="MLAVIGGTGLSQLASLERPRRQLVRTPYGEPSGPLTFGTLGATE
                     VVFLARHGYGHTLPPHQVNYRANLWALRDQGVDEIISVASVGGIRAGLEPGDLVVPDQ
                     IIDYTSGRRNTFFEDGAPVTHIDFTEPYTPALRRRLLEAAAACGEKAADGAVYAATQG
                     PRLETAAEIDRLERDGADLVGMTGMPEAALARELDLAYAALAVVVNHAAGRGDSARGI
                     RLRAIEEVMADAMRRVQRVIETAAAR"
     gene            complement(7583..8131)
                     /locus_tag="VI319_16290"
     CDS             complement(7583..8131)
                     /locus_tag="VI319_16290"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEY6865454.1"
                     /translation="MMPPQQAWSVLDSADLLCSEAQVRAAIVRMADEITEELEARYPL
                     VLAVMGGSVFFAGNLLPLLRFPLEFDYIHASRYGDATTGGRIVWKVEPGDNVRGRAVL
                     VLDDILDGGQTLAAIRERIMAGGASAFYCAVLTEKDLGARKPIEANFVGLRLPNRYVF
                     GCGMDVSGAWRNLPAIYAVKGS"
     gene            complement(8166..9026)
                     /gene="galU"
                     /locus_tag="VI319_16295"
     CDS             complement(8166..9026)
                     /gene="galU"
                     /locus_tag="VI319_16295"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY6865455.1"
                     /translation="MKVTKAVFPVAGMGSRFLPATKASPKEMMPIVDKPLIQYAFEEA
                     AAAGITDMIFITGRNKRAIEDHFDKAYELEAELAARGKSELLESVREVIPKSVNCIYI
                     RQAEPLGLGHAVLCARSVVGDQPFAVLLADDLIDAKRPVIGQMADLYAKDPYSILGVQ
                     EIPREQTDQYGIVAVEPRSGDLARITAIVEKPKPQAAPSTLAVVGRYILSPRIFGHLQ
                     KLQVGAGGEIQLTDAIAGLLAEEKVMAYRFEGRRYDCGSKLGYLQATVDFGTRHPEVG
                     NAFVSFLQSR"
     gene            complement(9023..11044)
                     /gene="ligA"
                     /locus_tag="VI319_16300"
     CDS             complement(9023..11044)
                     /gene="ligA"
                     /locus_tag="VI319_16300"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY6865456.1"
                     /translation="MAVPRKVQQRAAALRAEIEKHNYRYYVLDDPEIGDADYDRLFGE
                     LQALEREHPELATPDSPTQRVGGQPLAGFAPVTHGTPMLSLNNAFSEDDVLAFDRRAR
                     EALGVETIEYSAEPKFDGLAISLRYENGAFVQGATRGDGTTGEDVTANLRTVRSVPLR
                     LRGRAPGRLDVRGEVVMLKRDFEALNERQRAAGEKEYVNPRNSAAGALRQLDPRMTAA
                     RPLRFYGYGIGAAEQLPGAKTHAEVLERLADAGVPVSAERRVVRGADGVLAFYASLQS
                     RRAGLPYEIDGVVYKVNSIEQQARLGFVSRAPRFAVAHKFPAEEATTEIAGIEVNVGR
                     TGALTPVAKLKPVFVGGVTVTNATLHNEDEMRRKDVRIGDTVVVRRAGDVIPEIVRVL
                     PEKRPAGAELFQMPARCPVCGSSVVRLPDEAVARCSGGLFCAAQRKQALLHFASRRAM
                     DIEGLGEKLVDQLVEHGIVRTPADLYRLGVASLAELERMADKSAQNVVAAIDRSRQTT
                     LPRFVYALGIRNVGEATAADLARHFGGLDRLMDASEEELQQVRDVGPVVAMSARRFFS
                     ERHNREVIEQLRAGGVRWDEHAPQAAPAGALAGKTFVLTGTLPSLSRDDAKALIESRG
                     GKVAGSVSAKTNFVVAGDDPGSKLEKARSLGVAVLDEQGLMKLVEGAKA"
     gene            complement(11052..12110)
                     /locus_tag="VI319_16305"
     CDS             complement(11052..12110)
                     /locus_tag="VI319_16305"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEY6865457.1"
                     /translation="MSDLQLALLAAGVAGVAAVLAYNKWQELQYRRRAEAIFRAERED
                     VLLRAAVAEPAADRAEPFVPPPHAPAAENEPARSAAAPAVLSETLDCIVTLESPEGLA
                     GDAVLAAVDGVRHRCSKAVAWEGLDEEASRWQPLRADRGYSTLRAGLQLVDRRGAVTA
                     DELETFTSGVHDAAAGLGARAQLPDTRAALARAADLDRFSGDVDICIALNVVSAAAAF
                     PGARIRALAEAAGLALEPDGAFRRRDGSGRLLYELANLEPLAFDADSLKALTTRGLAL
                     QLDVPRAPGGERTFVQFADFAQQLARALGANIVDDNRKALGAPAFDAIRTQLKGVYKA
                     MESRGIEAGGALALRLFS"
     gene            complement(12116..>14297)
                     /locus_tag="VI319_16310"
     CDS             complement(12116..>14297)
                     /locus_tag="VI319_16310"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY6865458.1"
                     /translation="SRGELSRQEALLAQLETARATLDTGHQTALDQAQGLERELSALE
                     GRLLALQKIQSQVEDDGDVQEWLERHQLVSRPRLWQKIRVEPGWEAAVESALRERLHA
                     LDAGDLEGLERLVADPPPAKLSAFTPGIPTGLGRADGLRPLVSMVKALEPQVEGVMDE
                     WLAPYYAFDGTPRAADRESLPAGALLVNREGHQFSRFGVRFHAPDPADTGILAREREI
                     EALTAEIAEGQGRSRAAREHLAALDASLAEHDEGLAKLRAAGAALAQRQHERQLEHVK
                     LTQSQERHAGRNRQIEGELAEIAALRATEEAARTEAGNRLTRLDADLGTIGAAVNAVR
                     DEHAAAGDRLSEQREALQRAEREVQEGSFSERECSSKISEIERSLEVIREQKSTAEAS
                     AQSIGSELSGLKDDALRGQLQQALEVRVAKEQALGAARTRQDEIAARLRQWEEARAAC
                     EQKLQPLRDRIAELRLKEQAAHIAVDQFAQQLVEAGADVEALSGSLKEGQKAAPLQGE
                     ITRLTNAITELGAINMAALEELSTSQERKQFLDAQAADLATALETLENAIRKIDRETR
                     DLLQSTFDTVNRHFGEMFPVLFGGGESKLVMTGEEILDCGVQVIARPPGKRNTSIHLL
                     SGGEKALTAIALVFSFFQLNPAPFCLLDEVDAPLDDSNTLRFCDLVKKMSRQTQFLFI
                     SHNKITMEMANQLVGVTMQEQGVSRVVAVDIGEALKLREEREAA"
BASE COUNT         2053 a         5079 c         4823 g         2342 t
ORIGIN      
        1 cgtgcaccat cttcttctcc cagggcgtga tgccgatggt gcgcgcgtcg accagcgcaa
       61 cgttcgcgac cttggggatc ggcatggcgc tgccgtagta gtcgaccatc acgtggtcga
      121 ggatgcccgc gtgcgcgcgc ccggtccgca ccttggcgag gtcggccttg agcgcatcga
      181 gggacttctg catcttctgc tccgcagttt ttctcacatc cgcaagggtc gtcgccatgg
      241 cgcctccagc tcaatagtaa accagcgtgc cctctttttc gccggtgacg atgcgcttca
      301 tcgcccccgg cttgtagatg ctgaacaccg tgatcggcag cttctggtcg cggcacagcg
      361 cgaatgcggt cgcgtccatc acctcgaggt gcttgatcat cgcctcgtcg aagctgacct
      421 tgtcgtagcg cttcgcgtcc gggtggcgct tcgggtccgc cgtatacacg ccgtcgacct
      481 tggtcgcctt cagcaccatc tcggcgccga tctcggtgcc gcgcagcgcc gctgccgtgt
      541 cggtggtgaa gaacgggttt ccggtccccg cggcgaagat gacgatcttg ccctcctcga
      601 ggtagcggat cgccttgttg cggatgtaag gctcgaccac cgcctcgatg ttgagcgccg
      661 actgcacgcg ggcgttgagt cccgcctggc gcatcgcgtc ctgcagcgcc agcgcgttca
      721 tgatcgtcgc catcatgccg atgtagtcgg cggtcgcgcg gtccatgccc tgcgccccgg
      781 gggccacgcc ccggaagatg ttgccgccgc cgatcaccac gccgagctcc aggccgagcg
      841 ccgccacctg cccgatctcg ctgacgatgc gctcgatcgt ggcgcggttg atgccgtagg
      901 catcctcgcc catcagcgcc tcgccgctaa gcttcagcag cacgcgcttg aatttggcct
      961 gctcgggcat gactttagtg tgccttctcc gcctgcttgg cttgcgccgc tacttcggcg
     1021 gcgaagtcac tgcttttttt ttcgatccct tcaccgacca cgtagagcac gaaaccgccc
     1081 accgcggcgc ccttggactt cagcaacgct tcgatggact gcttttcatc cttgacgaag
     1141 ccctgtccca aaagcgtcac cgtcttgaaa aacttctgga ccgccccttc ggcgatcttc
     1201 gggacgagat cggccggctt gccgtcctcg atcgccttgg ccgttgcgat gtcacgctcc
     1261 ttcttcacga ggtcggcggg cacctggtct ttggaaagag ccaggggttt cgccgcggcg
     1321 atgtgcatcg acaggtcctt cgcgagctgc tcgtcgccgc ccgtcacgtc gaccatgaca
     1381 ccgatcttcg agccgccgtg gacgtattgc gcgattctgc ccttggcgtc gagccgcgcg
     1441 aagcgacgga cgctcaggtt ctcgccgatc tttccgacca gggctttgcg cacttcctcg
     1501 accgtcgacc cctggtacgg cagcgccgaa agcgccgcca cgtccgcggg cttcttctcg
     1561 agcaccagct tcgccagccc gttcgcgagc gcgatgaagt cgtcgttctt ggcgacgaag
     1621 tcggtctcgc agttcacttc gacgatcgcg ccggccttcg cgtcgcccga cagggcgatc
     1681 gcgacgatgc cttccgcggc gacgcgcgag gcggccttcg acgccttgtt ccccagcttg
     1741 atccggagga tctcctcggc cttgtccagg tcgccgctcg cttccgcgag cgccttcttg
     1801 cattccatca tcggggcgtc ggtcttgtcg cgcagcgcgc ggaccatgcc tgctgtgatt
     1861 tccgccattt cggtaatact ccgttacaaa cttcaggttc aaaaaaaggg gctgccgccc
     1921 ctttttgtgt ggaagccgcc ggccgcctta tcccgccttg ccttccgctt cctcgacctc
     1981 gacgaactcg tcggaggtcg cgcccacgat ctcctgcagc gactggctgc ggccttcgag
     2041 caccgcgtcc gcgacccctc gggcgtagag ccggatcgcg cggctggaat cgtcgttgcc
     2101 ggggatcacg tacgcgacgc cgtcgggcga atggttggtg tccacgacgc cgatcaccgg
     2161 gacgccgagc ttcttcgctt ccgcgaccgc gcccttctgg tagccgacgt cgaccacgaa
     2221 cagcgcgtcg ggcaggctgt cgagctgctt gatgccgccg aggctgcgct ggagcttctc
     2281 catctcgcgc tggaagtgca gcgcctcctt cttggacatc cggtccacgc tgccgtcctg
     2341 caccgccgcg tccatttcct tgagacgctt gatcgactgc ttgatcgtct tgaagttcgt
     2401 cagcatgccg ccgagccagc gatggtcgac gtacggcatg ccggcacgct cggcctcctc
     2461 gcgcatgatt tcgcgcgcct ggcgcttggt gccgacgaac aggatcgtgc ccttgttcga
     2521 cgacagctgg cggacgaatt tcatcgcttc ctggaactgg accatcgtct tttccaggtt
     2581 gatgatgtgg atcttgttgc ggtggccgaa gatgaaggga gccatcttcg ggttccagta
     2641 gcgggtctgg tggccgaaat gcacgcccgc ttccagcatc tgacgcatcg tggtcgacat
     2701 gaacgctccg gttagggttg atcgagagcc gctccgaccc aataaccctt tggtcgagga
     2761 ccgcggggca ccctaacggg ggaacggcaa aaatttagcc gagtccaaag ctcggccaga
     2821 cgacgatgat atcacggccc gtcgagggcg atcaagtttt tcctgtgcaa acagagtatt
     2881 atccgggaga tgcgaaacga ctacgacgtc cctgccggcg acgcgcgtaa ccgcatggcg
     2941 atccagctca agacgaccga agacatccag aagatgcgcg tcgcaggccg cctcgcggcc
     3001 gacgtgctcg acttcatcac cccgcacgtg cgtccgggca tcacgaccgg cgagctcgac
     3061 cgtctctgcc acgagtacat ggtcgacgag cagaagaccg tgcccgcgcc gctgaactac
     3121 gcgccccccg gatacaagcc gtttccgaag gccatctgcg cgtcggtgaa ccaccaggtg
     3181 tgccatgggg tgccgggcga ccgcgtgctc aaggcgggcg acatcctgaa cctcgacatc
     3241 accgtcatca aggacggctg gcacggcgac acgagccgca tgttctacgt gggcgagccc
     3301 agcatccagg cgcggcgcct gtgcgaggtc acctacgagt gcatgtggct cgggatccgc
     3361 agcgtgcgac cgggggcgca tctcggcgat atcggctcgg cgatccagca caacgccgag
     3421 tcgcacggct acagcgtcgt gcgcgagttc tgcgggcacg gcatcgggcg caaattccac
     3481 gaggagcccc aggtcctcca ctacggccgc gcgggcgcgg gcgtgaggct cgagccgggc
     3541 atgatcttca ccatcgagcc gatgatcaac gccggcaagg ccgcgatccg ggagctggcc
     3601 gacggctgga ccatcgtcac caaggaccac agcctatccg cgcaatggga gcacacggtg
     3661 ctcgtcacgc cggagggcta cgaggtcctg acgctctccc cgggatcgcc tccgccgcct
     3721 ccgccttcgt gacggcgctc gccccggcgg tccgtcccgc cccccgcgtc gacctcgccg
     3781 cgctgaaggg caacctggcg accgggcgcg cggcgctgcg cgagcgcttc gaggcgagcg
     3841 gcgacccggc cgccctgctg cgcggccact gccggctggt cgacggcgtc ctgcgcgacc
     3901 tgtggacgcg cctcgacatg cccgcggggt tcgcgctgct cgccgtcggc ggttacgggc
     3961 gcggcgagct gtttccgcat tcggacgtcg acatcctggt gctgctcccg ggcgaccctt
     4021 cgcccgggga cgcggcgaag gtcgaggagt tcatcggcgt cctctgggac tcgggcctcg
     4081 agacggggca cagcgtgcgc accgtccgcg actgcgtcga ggaggcggcc aaggacatca
     4141 ccgtgcgtac gagcatgctc gaggcgcgcc gcctcgcggg cggcgccggc ctgttccgcg
     4201 acttcgagga cgcggcgcgc gccgccctcc agacgccggt gtttttcaag gcgaagcgcc
     4261 tcgagcagga gcaacgccac accaagtacg ccgacagccc gtacagcctc gagccgaacg
     4321 tcaaggaggc gcctggcggg ctgcgcgacc tgcaggtcat cctgtggatc agccgtgcgg
     4381 ccgcgctcgg ccgcgactgg agcgagctcg cgcgccgcgg cctgatcacg acggcggagg
     4441 cgcgccaggc gaagcgcgac cagaggttcc tgcagcaggt gcgcgtgcgc ctgcactacg
     4501 ccaccgaccg gcgcgaggac cggctcctgt tcgactacca ggaagcggtc gcgcgcagta
     4561 tgggcatcgc ggcgaccgcg caccgccgcg cgagcgagga gctgatgcag cgctacttcc
     4621 gcacggcgaa gacggtcacg cagctgaaca ccatcctgct gcagaacctg ggcgccgaga
     4681 tcttcccgtc gcgcgagtcc gacgtcgaga tcatcgacga gcgcttccgg cgcgtgggcg
     4741 agctgctcga cgcgcgcggc gaggacctct tcgcgcgtca ccccgacgcg atcctcgaga
     4801 gctttctcgt gatgcagaag cgctcggagc tgaaaggcat gaccgcgcag acgctgcgcg
     4861 cgctgtggcg cgcgcgcgac cggatcgacg cccgctttcg cgcggatccc cggaatcgcg
     4921 cgactttcct cgagctgctg cagcagccgc ggggcatcgt ccacgagttc cggcgaatga
     4981 accagtacgg gatcctgggg cgctatctcc ccgcgttccg gcgcatcgtc ggacagatgc
     5041 agcacgacct gttccatgtc tacacggtcg atcagcacat cctgcaggtg gtgcgcaacg
     5101 tgcgccggtt cacgatgctc gagttcgcgc acgagtatcc cctttgcagc cggctgatcg
     5161 cgggcttcga gcgccactgg ctgctctacg tcgcggcgct gttccacgac atcgccaagg
     5221 gccgcggcgg ggaccattca aagctcggga agaccgaggc gcgccgcttc tgccgggacc
     5281 acggcatcgc cggcgaggat gccgagctgg tcgagttcct ggtcgagagc cacctgacga
     5341 tgtcgtcggt cgcgcagaag caggacctcg gcgatccgga caccgtgcgc gccttcgccg
     5401 acgtggtgaa aaccgagcgg cgcctggtgg cgctctacct gctcacggtc gccgacatcc
     5461 gcggcaccag ccccaaggtg tggaacgcgt ggaagggcaa gctccttgag gacctgtttc
     5521 ggctgactca gcgcctgctc cgcggcggga gcttcgaggc cgaccagtac atccacgaca
     5581 agcaggagga ggccaagcgc ctgctgcggc tgtacgcgct ctccgacacc gtccaggacg
     5641 cgctgtggaa gagcctcgac gtgccctact tcctgcgcca cgacgcgcag gagatcgcct
     5701 ggcacacgcg cacgctgcac taccgcgtcg acggcgacaa gccggtggtg aaggcacggc
     5761 tgtcgccggc cggcgaaggg ctgcaggtga tgatctacgt cgtcgaccag ccgaacctgt
     5821 tcgcgcgcat ctgcggatat ttcggctcga tccagttcaa catcgccgac gccaagatct
     5881 acaccacgcg caacggctac gcgctcgaca ccttccaggt gatggacacc ggcagcggcg
     5941 cccactaccg cgacatgatc gcgctgatcg agagcgagct cgccgactgg ctcgcgcgcg
     6001 agcacgagct gcccgcgccg atcaccggac gcgtgtcgcg ccgcgtgaag cacttcccga
     6061 tcgcgcccga ggtgcacatc gtgccggacg agcgcgcgca ctaccactcg ctcgcggtca
     6121 ccgcgggcga ccggcccggc ctgctctacg cggtggcgcg agtcctgggg aagtaccgca
     6181 tcgatctcca caccgcgaag atcgtgacgc tgggcgagcg cgccgaggac gtgttcgtgg
     6241 tctcgggctc cgcgctttcg aatccgaaga ccgtgctgca gctcgagacc gatctgctcg
     6301 aggcgctgca gtcctgatta ctcgtcgacg acgtcctgcc ccgggaacag cacgtcgacg
     6361 aacccgaact gcgagaagtc ctggatgcgc atgggataga ggatcccgtc caggtgatcg
     6421 cactcgtgct gcaccacgcg cgcgtggaag ccctcgaccg ttcggtcgat cggctcgccg
     6481 agggcgtcga agccggtata gcggatcgtg cggtagcgcg gcacgacgcc gcgcatgccg
     6541 ggtaccgaca ggcagccttc ccagccctcc tccatctcgc ggccgaccgg cttgacgacc
     6601 gggttgatga gcacggtgtc ggggacctcc tcggcgtccg ggtagcgcgg gttcgccttc
     6661 acgccgaaga tcacgacccg ctgcggcacg ccgatctgcg gcgcggcgag cccggcgccg
     6721 ttgagatgcg ccatggtttc gcgcatgtcg gcgatcagct cgcgcagctc ggccgtgcca
     6781 aaggcttcga cctcgcgcga gcgctcgagc aggcgcgggt cgcccatcct gagcacgggc
     6841 cggatcatcg cgccgccgcc gtctcgatga cgcgctgcac gcgccgcatg gcgtcggcca
     6901 tgacctcctc gatcgcgcgc aggcgtatgc cgcgggcgct gtcgccccgg cccgcggcgt
     6961 ggttgacgac gaccgcgagc gccgcgtacg cgagatcgag ctcgcgcgcg agcgcggcct
     7021 cgggcatgcc ggtcatgccg acgaggtccg cgccgtcgcg ctccaggcgg tcgatctcgg
     7081 ccgcggtctc gaggcgcggg ccctgcgtcg ccgcgtacac cgcgccgtcg gcggccttct
     7141 cgccgcacgc cgcggcggcc tcgagcagcc ggcggcgcag cgccggcgtg tacggctcgg
     7201 taaaatcgat gtgcgtcacg ggcgctccgt cctcgaagaa ggtgtttcgg cgcccgctgg
     7261 tgtaatcgat gatctgatcc ggcacgacca ggtccccggg ctccaggccc gcgcggatgc
     7321 cgccgaccga ggccaccgag atgatctcgt cgacgccctg atcgcgcagt gcccagaggt
     7381 tggcgcggta gttgacctgg tgcggaggca gcgtgtgtcc atagccgtgg cgtgcgagga
     7441 acaccacttc ggtggcgccc agggtcccga acgtgagcgg gccggacggc tcgccgtatg
     7501 gcgtcctcac gagctggcgc cgcgggcgct ccaggctggc gagctgcgac aggcccgtgc
     7561 cgccgatgac cgcgagcatt cctcaggaac ctttgaccgc gtagatcgcg ggcaggttgc
     7621 gccaggcccc cgagacgtcc atcccgcagc cgaagacgta gcggttcggc aggcgcaggc
     7681 cgacgaaatt cgcctcgatc ggcttgcgcg cgccgaggtc cttctcggtg agcacggcgc
     7741 agtagaaggc gctcgcgccg ccggccatga tgcgctcgcg gatggcggcc agcgtctgcc
     7801 cgccgtcgag gatatcgtcg agcaccagca cggcccgtcc gcgcacgttg tcgccgggct
     7861 cgactttcca gacgatgcgg ccgccggtgg tggcgtcgcc gtagcgcgac gcgtggatgt
     7921 agtcgaactc gagcgggaag cgcagcagcg gcagcagatt gccggcgaag aacacggacc
     7981 cgcccatcac cgcgagcacg agcgggtagc gggcctcgag ctcctccgtg atctcatccg
     8041 ccatgcgcac gatcgcggcg cgcacctgcg cttcgctgca gaggagatcg gccgagtcga
     8101 gaacgctcca ggcctgttgg ggcggcatca tggcggacgc gcgagtatac ccgctcgcgg
     8161 gcgccttacc gcgactgcag gaacgacacg aaggcattcc cgacttccgg gtgccgggtg
     8221 ccgaaatcca cggttgcctg gaggtagccg agcttggagc cgcaatcgta gcggcgacct
     8281 tcgaaccggt acgccatcac cttctcctcg gcgagcagcc ccgcgatcgc gtcggtcagc
     8341 tggatctcgc cgccggcgcc gacctggagc ttctgcagat gaccgaagat gcgcggcgag
     8401 aggatatagc gccccacgac cgccagcgtc gaaggcgcgg cctgcggctt cggcttttcg
     8461 acgatggcgg tgatgcgcgc gagatccccc gagcgcggtt ccacggcgac tatgccgtac
     8521 tgatcggtct gctcgcgcgg gatctcctgc accccgagga tcgagtacgg gtccttggca
     8581 tagaggtcgg ccatctgccc gatcaccggc cgcttcgcgt cgatgaggtc gtcggcgagc
     8641 agcacggcga aaggctggtc gccgaccacg ctgcgcgcgc acagcaccgc gtgccccagc
     8701 ccgagcggct cggcctggcg gatgtagatg cagttgaccg acttcgggat cacctcgcgc
     8761 acgctctcga ggagctcgct cttgccgcgc gcggcgagct cggcctcgag ctcgtaggcc
     8821 ttgtcgaagt gatcctcgat cgcgcgcttg ttgcgaccgg tgatgaagat catgtccgtg
     8881 atcccggccg cggctgcctc ctcgaaggcg tactggatga gcggcttgtc gacgatgggc
     8941 atcatttcct tcgggctcgc cttggtcgcc ggcaggaagc ggctgcccat gcccgcgacg
     9001 ggaaatacgg ctttggtcac cttcacgctt tcgctccttc caccagcttc atgagcccct
     9061 gctcgtcgag gacggcgacg ccgaggctgc gcgccttctc gagcttgctg cccgggtcgt
     9121 cgcccgcgac cacgaagttc gtcttcgcgg acaccgagcc cgcgaccttg ccgccgcgcg
     9181 actcgatcag cgccttcgcg tcgtcgcgcg acagcgaggg cagcgtgccc gtgagcacga
     9241 acgtcttccc ggcgagcgcg cccgcgggcg ccgcctgcgg cgcgtgctcg tcccagcgca
     9301 ccccgccggc acgcagctgc tcgatcacct cgcggttgtg ccgctcggag aaaaatcgcc
     9361 gcgccgacat cgcgaccacc gggccgacgt cccggacctg ctgcagctct tcctcgctcg
     9421 cgtccatcag ccggtcgagc cctccgaaat ggcgcgccag gtcggccgcg gtcgcctcgc
     9481 cgacgttgcg gatcccgagc gcgtacacga agcgcggcag cgtcgtctgc cgcgaccggt
     9541 cgatcgccgc caccacattt tgcgccgatt tgtcggccat gcgctcgagc tcggcgaggc
     9601 tcgccacgcc gagccggtac aggtcggccg gggtcctcac gatgccatgc tcgaccagct
     9661 ggtcaaccag cttctcgccc aggccctcga tgtccatcgc ccgccgcgac gcgaaatgca
     9721 gcagcgcctg cttgcgctgc gccgcgcaga acagcccgcc cgagcatctc gccaccgcct
     9781 cgtcgggcag gcgcaccacg ctcgagccgc acaccgggca gcgggcgggc atctggaaga
     9841 gctcggcgcc ggccgggcgc ttctcgggca gcacgcgcac gatctccgga atgacgtcgc
     9901 ccgcgcgccg gaccaccacg gtgtcgccga tgcgcacgtc cttgcggcgc atctcgtcct
     9961 cgttgtgcag cgtcgcgttc gtcaccgtga cgccgccgac gaacacgggc ttgagcttcg
    10021 cgaccggcgt gagcgcgccc gtgcgtccca cgttcacctc gatgccggcg atctcggtgg
    10081 tggcctcctc ggccgggaac ttgtgcgcga cggcaaagcg cggcgcgcgc gagacgaagc
    10141 cgagccgcgc ctgctgctcg atcgagttca ccttgtacac cacgccgtcg atctcgtagg
    10201 gcagcccggc gcggcgcgac tgcagggacg cgtagaacgc gagcacgccg tcggcgccgc
    10261 gcacgaccct gcgctcggcg ctgaccggca cgcccgcatc ggcgaggcgc tcgagcactt
    10321 cggcgtgcgt cttcgccccg ggcagctgct cggcggcgcc gattccgtag ccgtagaagc
    10381 gcagcgggcg cgcggccgtc atgcgcgggt cgagctgcct cagcgctccc gccgcggagt
    10441 tgcgcgggtt gacgtactcc ttctcgcccg ccgcgcgctg gcgctcgttc aaggcttcga
    10501 agtcgcgctt gagcatcacc acctcgccgc gcacgtcgag ccgcccgggc gcccggccgc
    10561 gaagccgcag cggaacgctg cggaccgtgc gcaggttggc cgtcacgtcc tccccggtgg
    10621 tgccgtctcc ccgcgtcgcg ccctgcacga acgcgccgtt ctcatatcgc aggctgatcg
    10681 ccaggccgtc gaacttgggc tccgcggagt actcgatcgt ttcgacgccg agcgcctcgc
    10741 gcgcgcgccg gtcgaaggcg agcacgtcgt cctccgagaa cgcgttgttg agcgacagca
    10801 tgggcgtccc gtgagtcacg ggcgcgaagc ccgcgagcgg ctgtccgccg acgcgctgcg
    10861 tcggcgagtc gggcgtggcg agctccgggt gctcgcgctc cagcgcctga agctcgccga
    10921 acagccggtc gtagtcggcg tcgccgatct cggggtcgtc gagcacgtaa tagcggtagt
    10981 tgtgcttctc gatctcggcg cgcagcgcgg ccgcgcgctg ctggaccttc cgcggtaccg
    11041 ccatcgccgt gtcaggagaa cagccgcagc gcgagcgcgc cgcccgcctc gatcccgcgg
    11101 gactccatgg ccttgtacac acctttgagc tgggtgcgta tcgcgtcgaa cgcgggcgcc
    11161 cccagcgcct tgcggttgtc gtcgacgatg ttggcgccga gcgcgcgtgc gagctgctgc
    11221 gcgaagtcgg cgaactgcac gaacgtgcgc tcgccgccgg gggcgcgcgg cacgtcgagc
    11281 tgcagcgcga ggccgcgcgt ggtcagcgcc ttcaggctat cggcatcgaa cgcgagcggc
    11341 tcgaggttcg cgagctcgta cagcagccgc ccggatccgt cgcggcggcg gaacgcgccg
    11401 tcgggctcga gggcgaggcc ggcggcctcg gcgagcgcgc gtatccgggc gcccgggaat
    11461 gcggccgcgg cgctcacgac gttgagcgcg atgcagatat cgacgtcgcc gctgaagcgg
    11521 tcgaggtccg cggcccgcgc gagcgcggcc cgggtgtcgg ggagttgcgc ccgcgcgccg
    11581 aggccggcgg cggcgtcgtg cacgcccgag gtgaacgtct cgagctcgtc ggcggtcacc
    11641 gcgccgcggc gatccacgag ctgcagcccc gcgcgcagcg tcgagtaccc gcggtcggcg
    11701 cgcaaaggct gccagcggga cgcttcttcg tcgagtcctt cccaggcgac agccttggag
    11761 cagcggtggc ggacgccgtc gaccgctgcg agcacagcgt cgccggcaag cccttcgggc
    11821 gactcgaggg tgacgatgca gtcgagcgtc tcggacagca cggccggagc cgcggccgag
    11881 cgggcgggct cgttctccgc cgccggagcg tgcggaggtg gaacgaaggg ctcggcgcga
    11941 tccgcggcgg gttcggcgac ggccgcgcgc agcaggacgt cctcgcgctc ggcgcggaag
    12001 atcgcctcgg cgcggcgccg atactgcagc tcctgccact tgttgtaggc gagcaccgcg
    12061 gccactcccg ccacaccggc cgccaacagc gcgagctgca ggtcgctcat gacgctcagg
    12121 ccgcctcgcg ctcttccctc agcttcagcg cttcaccgat gtcgaccgcc accacgcgcg
    12181 acacgccctg ctcctgcatc gtcacgccga cgagctggtt ggccatctcc atggtgatct
    12241 tgttgtggct gatgaacagg aactgcgtct gccgggacat cttcttgacc aggtcgcaga
    12301 agcgcaaggt gttgctgtcg tccagcggcg cgtcgacctc gtcgagcaga cagaagggcg
    12361 ccgggttcag ctggaagaag gagaacacga gcgcgatcgc ggtcagcgcc ttctccccgc
    12421 ccgagagcag atggatgctg gtgttgcgct tgccgggcgg gcgcgcgatc acctgcacgc
    12481 cgcagtcgag gatctcctcg cccgtcatga cgagcttcga ttcgccgccg ccaaagagca
    12541 ccgggaacat ctcgccgaaa tgccggttga cggtgtcgaa cgtgctttgc agcaagtcgc
    12601 gcgtctcgcg gtcgatcttt cggatggcgt tttcgagggt ctcgagcgcg gtggcgaggt
    12661 cggccgcctg cgcatcgaga aactgcttgc gctcctggct ggtcgagagc tcctcgagcg
    12721 cggccatgtt gatggcgccg agctcggtga tggcgttcgt gagccgcgtg atctcgccct
    12781 gcagcggcgc ggccttctgc ccctccttca gcgagccgga gagcgcctcg acgtcggcac
    12841 ccgcctcgac gagctgctgc gcgaactggt cgacggcgat gtgcgcggcc tgctccttca
    12901 gccgcagctc ggcgatgcgg tcgcgcaggg gctggagctt ctgctcgcac gccgcgcgcg
    12961 cctcctccca ctgccgcagg cgggcggcga tctcgtcctg tcgggtccgc gcggcgccga
    13021 gcgcctgctc cttcgcgacc cggacctcga gcgcctgctg gagctggccg cgcagcgcgt
    13081 cgtccttcag tcccgagagc tcgctgccga tcgactgggc ggaagcctcc gcggtggatt
    13141 tttgctcgcg tatcacttca agagagcgct ctatttcact gatcttactg gaacattctc
    13201 tctcggaaaa ggacccttcc tggacttcgc gctcggcccg ctgcagcgcc tcgcgctgct
    13261 ccgacagacg gtcgccggca gccgcgtgtt cgtcgcggac ggcgttcacg gccgcgccga
    13321 tcgtcccgag atccgcatcg agccgggtca gtcggttgcc ggcctcggtg cgcgcggctt
    13381 cctccgtggc gcgcagggcg gcgatctcgg ccagctcgcc ctcgatctgg cggttgcgcc
    13441 cggcatggcg ctcctggctc tgcgtgagct tgacgtgctc gagctgccgc tcgtgctggc
    13501 gctgcgcgag cgcggcgccc gccgcgcgca gcttggcgag tccttcgtcg tgctcggcga
    13561 gcgaggcgtc cagcgcggcg aggtgctcgc gcgccgcgcg cgagcggccc tgcccttcgg
    13621 cgatctcggc cgtcagcgcc tcgatctcgc gctcgcgcgc gaggatcccg gtgtcggcgg
    13681 gatcgggcgc gtggaagcgc acgccgaagc ggctgaactg gtgtccttcg cgattcacca
    13741 ggagcgcgcc ggcgggaagc gactctcggt cggccgcgcg cggggtgccg tcgaacgcgt
    13801 aatacggcgc gagccactcg tccatcacgc cctcgacctg cggctccagg gccttgacca
    13861 tgctcacgag cggacgcagg ccgtcggcgc gcccgagccc cgtcggaatg cccggcgtga
    13921 acgcgctgag cttcgccggc ggcgggtcgg cgacgagccg ctcgagccct tcgaggtcac
    13981 cggcgtcgag cgcgtgcagc cgctcgcgca gcgccgattc caccgcagcc tcccagcccg
    14041 gctcgacgcg gatcttctgc cacagccgcg ggcgcgagac gagctggtgg cgctcgagcc
    14101 attcctggac gtcgccgtcg tcctcgacct ggctctggat cttctgcagc gcgagcagcc
    14161 gtccctcgag cgccgagagc tcgcgctcga gcccctgcgc ctggtcgagg gcggtctggt
    14221 gcccggtgtc gagggtcgcg cgcgccgtct cgagctgcgc aagcagcgct tcctggcgcg
    14281 acagctcccc gcggctc
//
LOCUS       DATDMW010000391         8570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_117662, whole genome shotgun sequence.
ACCESSION   DATDMW010000391 DATDMW010000000
VERSION     DATDMW010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117662"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..565)
                     /locus_tag="VI319_16315"
     CDS             complement(77..565)
                     /locus_tag="VI319_16315"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY6865459.1"
                     /translation="MDKTATRRGLVLFFRLAMAWTFIYAASHQVFDPAWSVAGFLKGT
                     KTFHDVYAPFTTERWAPVITFLVGYGHLLIGLSLLFGLFVRVSASFGILLMLVYWTAH
                     MSFPYIENKNNFLLDFHIVYAGVLLQLIVERAGHVWGLDGWAERIDFFGRHPKLKAAL
                     VA"
     gene            complement(678..1634)
                     /locus_tag="VI319_16320"
     CDS             complement(678..1634)
                     /locus_tag="VI319_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865460.1"
                     /translation="MRPLVRFAVLVLVVAPLAAAAEYPTRAVKWVVPYPPGGTTDVLA
                     RLTSNWLAEQLHQTFLIENRPGGGNNIGVDYVVRSAPDGYTMLLVNPANGINTTLYKN
                     LPFNFLRDIAPVAGLVRTPNVMEVTTSLPVRTVAEFIDYCKKNPGKINMASSGSGTSV
                     HLSGELFKSMTGCQMLHVPYKGAGPALADLIPGQVHVMFDNLPSSIGHIRGGKLRALA
                     VTSAERDAALPDVPTVAETLPGYEATAWFGIGMPKGSPREAIDKVNAAVNKALADPGM
                     LKRYAELGGKPMKGTPEDFGRVIAAETAKWEKVVIFSGAKAE"
     gene            1928..2137
                     /locus_tag="VI319_16325"
     CDS             1928..2137
                     /locus_tag="VI319_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865461.1"
                     /translation="MSRYWRDNPFACDTAEGIRQWWLAPADEISIEQVHVALAWLCDR
                     GVAERLLAADGRPRFRRSGTRRRAT"
     gene            2141..2722
                     /locus_tag="VI319_16330"
     CDS             2141..2722
                     /locus_tag="VI319_16330"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEY6865462.1"
                     /translation="MRADMIDVAAAFLVKELNAYMLARTGTSFGSALLSRVVGDNGKW
                     AIPDDQVGVALVSVEEERALRSQVPQTAYADGRQVVLEPELKLNLHLLVAANFKQYDQ
                     GLRQLALVLTFFQSHAVFTRERNPGLDPRIEKLGVDLLSLTFEQLNQLWTFVGAKQLP
                     SAVYRVRLIALQDLQPSAVQPPITTIGSSIEAI"
     gene            2719..3543
                     /locus_tag="VI319_16335"
     CDS             2719..3543
                     /locus_tag="VI319_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865463.1"
                     /translation="MTIEFLPLLTLEATHDYYGGRCPDVAFWMPSGTQRLLAGAHVLA
                     KPREDRLNVLFERKDGGGAFEPRFSLAGSKLQIGLKLLNPEFANFTVLPLVAGGGLPL
                     YRSGPSPGALQAPVGLLLDPAHPDDAELLREGLLCLVEITLGAGFYAAPPAFAVPFAA
                     RAETLKYYVVARNYTAGEFAQIDVSDTGFGADARPQIHFDRVASSAFGAAEIPATQLG
                     APDARVALFRSQQPVARRSKARTRIQLARNNDVIVAQLPQPGAAAATADLVVHLSK"
     gene            3553..5025
                     /locus_tag="VI319_16340"
     CDS             3553..5025
                     /locus_tag="VI319_16340"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEY6865464.1"
                     /translation="MPSNLVTPGVYVKEIKVFPPSVAEVESAVPAFVGYTQTAKKDAN
                     NDLILKPTPIFSMTQFEQYYGGAQVPEYDITITETSDGVKTSTLTAPAAPVPGFVVLP
                     DYLLHHAVRMYFENGGGGPAYVVSVGLYQDTPAFDLTGDGGTDPATAYGLQDGLDAVA
                     LEDEPTLLVIPEAVKLTYAKYLTLTQAMLGQASNLGDRFAILDAFDGYKGTLDNVNTG
                     GGTKSVVAANRDAWPVDTDILKYGAAYYPFVKTAMNPPFDETTVKVTLPAGGTAVTLA
                     SLKNSDTANYNEAKALLAKVFLVLPASGAVTGVYAYTDTARGVWKAPANVSLNDVLEP
                     VVKLDDTAQGPLNVDPTSGKSINALRAFAGKGTLVWGARTLAGNDNEWRYVNVRRFFN
                     MVEESVKKSTYWAVFESNDSNTWVKVRGMIESYLTQKWREGALAGATPKDAFFVRVGL
                     GTTMDAQDILEGRMNVEIGMAVVRPAEFIILKFSHKMQVS"
     gene            5042..5479
                     /locus_tag="VI319_16345"
     CDS             5042..5479
                     /locus_tag="VI319_16345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY6865465.1"
                     /translation="MPAQYPLPVFHFTVQWGGTRVGFSEVTGLTQENQAIEYRDGSFP
                     EYSSIKMPGLRKFSNLTCKRGVVRGDNDFANWLATVKLNTVERRDLVVSLLNEEHTPV
                     MTWKVHNAFPVKVEGPQLKASGNEVAIESIELAHEGLEVQNEA"
     gene            5479..5940
                     /locus_tag="VI319_16350"
     CDS             5479..5940
                     /locus_tag="VI319_16350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY6865466.1"
                     /translation="MPHYYPPKAFHFKVEVQGLESADEVRFTDVGGLSVEMATEEVPE
                     AGENRFVQKYPVRAKYPELVLKRGLLPRSAVFGWIRDAIQDYNIVPRDVDVKLLDDEH
                     QPLVTWHLVKAYPTKWSVADLNASGNAVSVETLQMFYQYFTVDASAVPPEA"
     gene            5942..6109
                     /locus_tag="VI319_16355"
     CDS             5942..6109
                     /locus_tag="VI319_16355"
                     /inference="COORDINATES: protein motif:HMM:NF039278.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HEY6865467.1"
                     /translation="MPVIVDEIVISVEVAPQDGAPGAPAAAAGAPDAQRALVAECVER
                     VLDVLARREEP"
     gene            6112..6780
                     /locus_tag="VI319_16360"
     CDS             6112..6780
                     /locus_tag="VI319_16360"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865468.1"
                     /translation="MIDDKGELNRLTIRGYVKADCKGDFVEYKAYVNPGEVTLGFEAD
                     YAQQTGAGNTPGPQHFNRIKSGDLTVALFLDGTGANGRKVVVQDEIQAFMNATGYDGQ
                     GHEPRYLLVGWGKLPVFRCRLKSASTVYKLFTPGGVPLRAVITATFTETVDAGTSERK
                     AGNESADLTHLRRVQAGDTLPALCQAIYGEPRLYLDVARANALDNFRALVPGTQLRFP
                     PLAK"
     gene            6782..8479
                     /gene="vgrG"
                     /locus_tag="VI319_16365"
     CDS             6782..8479
                     /gene="vgrG"
                     /locus_tag="VI319_16365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEY6865469.1"
                     /translation="MPAAARTLPISSEHREFTVKVGGQAVGREHRLAAVHVTKAVNRI
                     SSASLHYYDGSAAAGDLPLSNTGTFVPGADVEILAGTPDDPASIFKGIVVRQSIRVRD
                     HAAPMLIVECRHAATKLTVGAKNAFYVGEKDSDVIAALLAAANVDADVEATSVSHPQQ
                     VQFRATDWDYLLARAEANGKMVFTNDAKVAVRAPVVSGTAAVSLLFGATIMELDAEID
                     ARSQFSAVKSLTWDPAQQSVLAKDASDPGVPGPGNLSSDDLAGVAALPALELRHAALE
                     GDEAQAWADAQWLRSKLSKVNGRAKCEGIATVNPGDLVDLSGVGDRYSGTAFVTGVRH
                     EYDAVQGWKTHLQFGSVDRWSAEEREISAPPAAALLPAVNGLQIGQVTGNEDPDGEHR
                     VRVRLPLVNDQADGVWARVASLDAGDQRGFFFRPELGDEVVVGFLDDDPRRAVILGML
                     HSSAKAAPLQGSDDNNEKMYRSRSGMRLYFDDDKKIALLETPAGNRLTLSEQDQGVKL
                     EDQNGNKIEMSASGVKIESASALELKAGSQLKLSGQAGAELSSSATCKVQGSMVQIN"
BASE COUNT         1393 a         3071 c         2890 g         1216 t
ORIGIN      
        1 tgcgctcgtt cgaggaaagc gcagcggaga cgaaaagccc ggaccggccg gtccgggccc
       61 gtgcggcgcg cgcggtctag gcgaccagcg ccgccttcag cttcgggtgg cgcccgaaga
      121 agtcgatccg ctcggcccag ccgtccagtc cccagacgtg gcccgcgcgc tcgacgatga
      181 gctgcagcag caccccggcg tagacgatgt ggaagtcgag caggaagttg ttcttgttct
      241 cgatgtacgg gaagctcatg tgcgcggtcc agtacaccag catgagcagg atcccgaagg
      301 acgcgctcac gcgcacgaac aggccgaaca gcagcgacag cccgatcagc aggtgcccgt
      361 agcccacgag gaacgtgatc accggcgccc agcgctcggt ggtgaacggc gcgtagacgt
      421 cgtggaaggt cttcgtgccc ttgaggaacc cggcgacgct ccaggccggg tcgaacacct
      481 ggtgcgaggc ggcgtagatg aaggtccacg ccattgccag gcggaagaag aggaccaggc
      541 ccctcctcgt tgcggtcttg tccataccgg tctcctgtcg ttccggccgc gccatccgcg
      601 gcccacgatc gcagtatccg cccgccgcgg acggcggatt tgattcaaat caaatccgca
      661 cgaccgggat cacgcggtca ttccgccttg gcgcccgaga agatcacgac cttctcccac
      721 ttcgcggtct cggccgcgat cacgcggccg aagtcctcgg gcgtcccttt catcggcttg
      781 ccgcccagct cggcgtagcg cttcagcatg cccggatcgg ccaacgcctt gttcaccgcg
      841 gcgttcacct tgtcgatcgc ctcgcgcggc gagcccttcg gcatgccgat gccgaaccaa
      901 gccgtcgcct cgtagccggg aagcgtctcc gcgacggtcg gcacgtcggg cagcgccgcg
      961 tcgcgctcgg ccgaggtgac cgcgagcgcg cgcagcttgc ccccgcggat gtgcccgatc
     1021 gacgacggca ggttgtcgaa catcacgtgc acctgtccgg ggatgaggtc ggcgagcgcc
     1081 gggcccgcgc ccttgtacgg gacgtgcagc atctggcagc cggtcatcga cttgaacagc
     1141 tcgcccgaga ggtgcaccga cgtgccgctg cccgaggacg ccatgttgat cttcccggga
     1201 ttcttcttgc agtagtcgat gaactcggcg accgtcctca ccggcagcga cgtcgtcacc
     1261 tccatcacgt tgggcgtgcg cacgaggccg gcgactggcg cgatgtcgcg cagaaagttg
     1321 aatggcaggt tcttgtacag cgtcgtgttg atgccgttcg cgggatttac cagcagcatc
     1381 gtgtagccgt cgggcgccga gcgcacgacg taatcgacgc cgatgttgtt gccaccgccc
     1441 ggccggttct cgatcaggaa ggtctggtgc agctgctcgg cgagccaatt ggaagtcagg
     1501 cgcgcgagca cgtcggtcgt gccgcccggc gggtaaggca ccacccattt caccgcgcgg
     1561 gtcgggtatt cggccgcggc ggcgagcggc gccacgacga ggacgagcac ggcgaagcgg
     1621 acgagcggtc gcatggcggc tcctttttcc caggagccgc catcgtagcg gaagtcgcgc
     1681 ggacaaaggc gcccgcgcgc ccggacgaac tcgtccgggc tcccgcgccg ctgcgccggg
     1741 cggctcgggc gctcaccccg cccggacgga aaggtccagg cgcgcgcgcc gcgccctgca
     1801 cctggaccgc ggcgaatcag gcacttgccc ggcgcgatcc cctggcccct tgcttgcaag
     1861 ggaatcccgg gagaactctc tcatggcggg ccgtgctcga ttctcaacgc aacaggtcgt
     1921 cagatcgttg tcgcgctact ggcgcgacaa ccccttcgcc tgcgacaccg ccgagggcat
     1981 ccgccagtgg tggctcgccc cggccgacga gatctcgatc gagcaggtgc acgtcgcgct
     2041 cgcgtggctg tgcgatcgcg gcgtggccga gcggctcctc gccgccgacg gccgtccgcg
     2101 cttccggcgc tccggcacgc gccggcgcgc aacctgagcg gtgcgcgcgg acatgatcga
     2161 cgtcgccgcc gcgttcctgg tcaaggagct gaacgcgtac atgctcgcgc gcaccggcac
     2221 gagcttcggc agcgccctgc tctcgcgcgt ggtcggcgac aacggcaagt gggcgatccc
     2281 ggacgaccag gtcggcgtcg cgctggtaag tgtcgaggag gagcgcgcgc tgcgctccca
     2341 ggtgccccag accgcgtacg ccgacggccg ccaggtggtg ctcgagcccg agctcaagct
     2401 gaacctgcac ctgctcgtcg ccgcgaactt caagcagtac gaccaggggc tgcgccagct
     2461 cgccctggtg ctcacgttct tccagtcgca cgcggtgttc acgcgcgagc gcaaccccgg
     2521 cctcgacccg cgcatcgaga agctcggcgt ggacctgctc tcgctcacgt tcgagcagct
     2581 gaatcagctc tggaccttcg tcggcgccaa gcagctgccc tcggccgtgt accgcgtgcg
     2641 cctgatcgcg ctgcaggacc tgcagccctc cgcggtgcag ccgccgatca cgaccatcgg
     2701 ctcgagcatc gaggcgatat gacgatcgag ttcctgcccc tgctcacgct cgaggcgacg
     2761 cacgactact acggcgggcg ctgccccgac gtcgcgttct ggatgccctc cggcacacag
     2821 cgcctgctcg cgggcgcgca cgtcctcgcc aagccgcgcg aggaccgcct caacgtgctg
     2881 ttcgagcgca aggacggcgg cggcgcgttc gagccccggt tctcgctcgc aggcagcaag
     2941 ctgcagatcg ggctgaagct cctgaacccg gagttcgcga acttcaccgt gctgccgctc
     3001 gtcgcgggcg gcggcctgcc gctgtaccgc agcggcccga gcccgggcgc gctgcaggcg
     3061 ccggtcgggc tgctgctcga ccccgcgcac ccggacgacg ccgagctgct gcgcgagggg
     3121 ctgctgtgcc tggtcgagat cacgctgggc gcgggcttct acgccgcgcc ccccgccttc
     3181 gccgtgccgt tcgcggcgcg cgcggagacg ctcaagtact acgtcgtcgc gcgcaactac
     3241 accgccggcg aattcgcgca gatcgacgtc agcgacaccg gcttcggcgc cgacgcgcgc
     3301 ccgcagatcc atttcgaccg cgtcgcctcg tccgccttcg gcgcggccga gatcccggcg
     3361 acgcagctcg gcgcgcccga cgcgcgcgtc gcgctgttcc gctcgcagca gcccgtcgca
     3421 cgccggtcga aggcgcggac ccgcatccag ctcgcccgca acaacgacgt catcgtcgcg
     3481 cagctccccc agcccggcgc cgcggccgcg accgcggacc tggtggtcca cctgtccaaa
     3541 tagaggtccc acatgcccag caacctcgtc acacccggcg tctacgtcaa ggagatcaag
     3601 gtctttccgc cttcggtcgc ggaagtcgag tcggcggttc ccgccttcgt cggctacacg
     3661 cagacggcga agaaggacgc caacaacgat ctgatcctca agccgacgcc gatcttctcg
     3721 atgacgcagt tcgagcagta ctacggcggg gcgcaggtgc ccgagtacga catcaccatc
     3781 accgagacga gcgacggcgt gaagacctcg acgctcaccg cgcccgccgc cccggtgccg
     3841 ggcttcgtcg tcctgcccga ctacctgctc catcacgccg tgcgcatgta cttcgagaac
     3901 ggcggcggcg gcccggcgta cgtcgtctcg gtcggcctgt atcaggacac gccggcattc
     3961 gacctgaccg gcgacggcgg caccgacccg gccacggcct acggccttca ggacgggctc
     4021 gacgccgtcg cgctcgagga cgagccgacg ctgctcgtca tccccgaggc ggtcaagctc
     4081 acctacgcca agtacctgac gctgacgcag gcgatgctcg gccaggcgag caacctcggc
     4141 gaccgcttcg cgatcctcga cgccttcgac ggctacaagg gcacgctcga caacgtgaac
     4201 accggcggcg gcacgaagag cgtcgtcgcg gccaaccgcg acgcctggcc ggtcgacacc
     4261 gacatcctca agtacggagc ggcgtactac ccgttcgtga agacggcgat gaacccgccc
     4321 ttcgacgaga ccaccgtcaa ggtgacgctg cccgcgggcg gcacggcggt caccctcgcc
     4381 tcgctcaaga acagcgacac cgcgaactac aacgaggcga aggcgctgct cgcgaaggtg
     4441 ttcctggtgc tgcccgcgag cggagcggtc accggcgtct acgcctacac cgacaccgcg
     4501 cgcggcgtgt ggaaggcgcc ggccaacgtc tcgctgaacg acgtgctcga gcccgtcgtc
     4561 aagctcgacg acaccgcgca gggcccgctc aacgtcgacc cgacctcggg caagtcgatc
     4621 aacgcgctgc gcgccttcgc gggcaaggga acgctcgtct ggggcgcgcg cacgctcgcc
     4681 ggcaacgaca acgagtggcg ctacgtgaac gtgcgccgct tcttcaacat ggtcgaggaa
     4741 tcggtgaaga agtcgaccta ctgggcggtg ttcgagagca acgactcgaa cacctgggtg
     4801 aaggtgcgcg gcatgatcga gtcctacctc acgcagaagt ggcgcgaggg cgcgctggcc
     4861 ggcgcgacgc ccaaggacgc gttcttcgtg cgcgtcggcc tcggcacgac gatggacgcc
     4921 caggacatcc tcgaaggccg catgaacgtc gagatcggca tggccgtcgt gcggcccgcc
     4981 gagttcatca tcctgaagtt ctcgcacaag atgcaggtgt cctgaaagga gccctccagc
     5041 catgcccgca caatatccgc ttcccgtctt ccacttcacc gtgcagtggg gcggcacccg
     5101 cgtcggcttc tccgaggtca ccggcctcac gcaggagaac caggcgatcg agtaccgcga
     5161 cggctcgttc cccgagtact cgtcgatcaa gatgccgggg ctgcgcaagt tctccaacct
     5221 cacgtgcaag cgcggcgtcg tcaggggcga caacgacttc gccaactggc tcgccaccgt
     5281 caagctgaac acggtcgagc gccgcgacct ggtcgtgagc ctgctcaacg aggagcacac
     5341 gccggtcatg acctggaagg tgcacaacgc cttcccggtc aaggtcgagg gcccgcagct
     5401 caaggcgagc ggcaacgagg tcgcgatcga gtcgatcgag ctcgcgcacg agggcctcga
     5461 ggtccagaac gaggcctgat gccgcactac tatccaccga aggccttcca cttcaaggtc
     5521 gaggtgcagg ggctggagag cgcggacgaa gtgcgcttca cagacgtcgg cgggctctcg
     5581 gtcgagatgg ccaccgagga ggtgcccgag gccggcgaga accgcttcgt gcagaagtac
     5641 ccggtgcgcg cgaagtatcc cgagctcgtc ctcaagcgcg ggctgctgcc gcgctcggcg
     5701 gtgttcggat ggatccgcga tgcgatccag gactacaaca tcgtgccgag ggacgtcgac
     5761 gtgaagctgc tcgacgacga gcaccagccg ctcgtcacct ggcacctcgt gaaagcgtac
     5821 ccgacgaagt ggtcggtggc cgacctcaac gcctcgggca acgcggtgtc ggtggagacg
     5881 ctgcagatgt tctaccagta cttcacggtc gacgcctcgg ccgtgccgcc ggaggcctga
     5941 ggtgcccgtc atcgtcgacg agatcgtgat ctcggtggag gtcgcgccgc aggacggcgc
     6001 gcccggcgcc cccgccgcgg ccgcgggcgc gccggacgcg cagcgcgcgc tcgtcgccga
     6061 gtgcgtcgag cgcgtgctcg acgtgctcgc ccggcgcgag gagccctgat cgtgatcgac
     6121 gacaagggcg agctgaaccg cctcaccatc cgcggctacg tcaaggcgga ctgcaagggc
     6181 gacttcgtgg agtacaaggc ctacgtcaac cccggggagg tcacgctggg attcgaggcc
     6241 gactacgcgc agcagacggg cgccggcaac acgcccggcc cgcagcactt caaccgcatc
     6301 aagtcgggcg acctcacggt cgcgctcttc ctcgacggca ccggcgcgaa cggccgcaag
     6361 gtcgtcgtcc aggacgagat ccaggcgttc atgaacgcga ccggctacga cggccagggc
     6421 cacgagccgc gctatcttct ggtcggctgg gggaagctgc ccgtgttccg gtgccgcctg
     6481 aagagcgcct ccaccgtcta caagcttttc acgccgggcg gcgtgccgct gcgcgccgtg
     6541 atcaccgcga ccttcaccga gacggtcgac gccggcacca gcgagcgcaa ggccgggaac
     6601 gagtccgcgg acctgacgca cctgcgccgg gtccaggcgg gcgacacgct gcccgcgctg
     6661 tgccaggcga tctatggcga gccgcgcctg tatctcgacg tggcgcgcgc caacgcgctc
     6721 gacaacttcc gcgcgctcgt gcccggcacg cagctgcgct tcccgccgct cgcgaaatag
     6781 catgcccgcc gccgcccgca cgctgccgat ctcctccgag caccgcgagt tcacggtgaa
     6841 ggtcggcggg caggccgtgg gccgcgagca ccggctcgcg gccgtgcacg tcaccaaggc
     6901 ggtgaaccgg atctcgtcgg cgagcctgca ttactacgac ggctcggcgg cggcgggcga
     6961 tttaccgctc agcaacaccg gcacgttcgt tcccggcgcc gacgtcgaga tcctcgccgg
     7021 aacgcccgac gacccggcgt cgatattcaa gggcatcgtc gtgcgccagt cgatccgcgt
     7081 gcgcgaccac gccgcgccga tgctgatcgt cgagtgccgc cacgcggcga cgaagctcac
     7141 cgtcggcgcg aagaacgcct tctacgtcgg cgagaaggac agcgacgtca tcgccgcgct
     7201 gctcgcggcc gcgaacgtcg acgccgacgt cgaggcgacg agcgtgagcc acccgcagca
     7261 ggtgcagttc cgcgccaccg actgggacta cctgctcgcg cgcgccgagg cgaacggcaa
     7321 gatggtcttc accaacgacg cgaaggtcgc ggtgcgcgcg cccgtcgtct cgggcacggc
     7381 ggcggtgtcg cttcttttcg gcgcgaccat catggagctc gacgccgaga tcgacgcgcg
     7441 ctcgcagttc tccgcggtga agagcctcac ctgggacccg gcgcagcaga gcgtgctcgc
     7501 gaaggatgcg tccgacccgg gcgtgcccgg cccggggaac ctgtcgagcg acgacctcgc
     7561 cggcgtcgcg gcgctgcccg cgctcgagct gcgccacgcg gcgctcgaag gcgacgaagc
     7621 gcaggcctgg gccgacgcgc agtggctgcg ctcgaagctc tcgaaggtga acggccgcgc
     7681 caagtgcgag ggcatcgcca cggtgaatcc cggggacctc gtcgacctct ccggcgtcgg
     7741 cgaccgctac agcgggaccg cgttcgtcac cggcgtgcgc cacgagtacg acgccgtgca
     7801 gggctggaag acgcacctgc agttcggctc ggtcgaccgg tggtccgccg aggagcgcga
     7861 gatctccgcg ccgcccgccg cggcgctcct gcccgccgtg aacggcctgc agatcggcca
     7921 ggtcacgggc aacgaggatc ccgacggcga gcaccgcgtg cgcgtgcgcc tgccgctcgt
     7981 caacgaccag gccgacggcg tgtgggcgcg cgtcgcctcg ctcgacgcgg gcgaccagcg
     8041 cggcttcttc ttccggcccg agctcggcga cgaggtcgtg gtcggcttcc tcgacgacga
     8101 tccgcgccgc gccgtgatcc tcgggatgct gcactcgagc gcgaaggccg cgccgctgca
     8161 gggctcggac gacaacaacg agaagatgta ccgcagccgc tcgggcatgc gcctgtactt
     8221 cgacgacgac aagaagatcg cgctgctcga gacgcccgcc ggcaaccggc tcacgctctc
     8281 ggagcaggac cagggcgtga agctcgagga ccagaacggc aacaagatcg agatgagcgc
     8341 ctcgggcgtg aagatcgaga gcgcgagcgc gctcgagctg aaggcgggct cgcagctgaa
     8401 gctctccggc caggccggcg ccgagctgtc gagctcggcg acctgcaagg tgcagggcag
     8461 catggtgcag atcaactagg cggcgcgatg cccgcggcgg cccgactcgg cgacaccagc
     8521 acccacggcg ggacgctcac cggcccgggc gtggcgaccg tgctgatcgg
//
LOCUS       DATDMW010000392         5131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_614777, whole genome shotgun sequence.
ACCESSION   DATDMW010000392 DATDMW010000000
VERSION     DATDMW010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614777"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VI319_16370"
     CDS             complement(<1..416)
                     /locus_tag="VI319_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865470.1"
                     /translation="MKSIYRWLLPAALCALAAGPLRAQSADIAGLAQYAGPDREQKLA
                     QAAKKERQVTLYSSLVVEDLTAIAAAFQKKYGVKVSYWRAGSEKVVQRSVTEARAGRF
                     DVDVIETNGPELESLHREKLLQKAGSPQFKDLLPDA"
     gene            complement(476..910)
                     /locus_tag="VI319_16375"
     CDS             complement(476..910)
                     /locus_tag="VI319_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY6865471.1"
                     /translation="MSKLTLKQANLIVDKALERARQMKIKPLAVVVLDDAGNVKAAQR
                     EDGASMFRFDVALGKAWASVAMGASSRALLKRAKDNPNFFVTLAATAKGKFLPQTGGV
                     LIKDAQGEILGAAGASGGTGEEDEEACVHGIQQAGLTPDASG"
     gene            1025..2200
                     /locus_tag="VI319_16380"
     CDS             1025..2200
                     /locus_tag="VI319_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865472.1"
                     /translation="MNRTVAAVLGALACFAASGALAQQPVRIGLIMEYSGPFADAAAQ
                     MDNGVKLYMKQHGDTVGGKKIEIIRRDTGGPAPDVSKRHAQELVVRDHVDVLAGFVLT
                     PNALAAADVSAEAKKFTVVMNAATSIITTKSPYLVRTSLTIPQVVEPLGSWAYRNGVR
                     KAYTLVSDYGPGIDAEGAFQRAFKAAGGEISGSARTPLANPDFFPFVQRAKDLNPEAM
                     FIFVPGGAQPAALGKAFVDLGMDSKKTKIMGQGEITEENAIKVMGDAALGLITAFHYD
                     YNHKSAMNAKFVKEYNAAFGRNPDFFTVGGYDGMHLIYEALKKAKGKTDGDSLVAAAK
                     GMTWESPRGPMSIDPETRDVVQTVYIRRVEKVGGKLVNVEIDKIENVKDPVKDRMKK"
     gene            2214..3137
                     /locus_tag="VI319_16385"
     CDS             2214..3137
                     /locus_tag="VI319_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY6865473.1"
                     /translation="MFNPFQEIERVEVKLFTSMPAKFRKKGRTAWSDPNRGGAEVDSF
                     LEGPSFDRHGNLYCVDIPFGRVFRIDPKGEWDLVVQYDGWPNGLKIHKDGRIFIADYK
                     RGLMGLDAKSGRIEPLVETAFSESFKGLNDLHFADNGDLYFTDQGQTGIADPTGRVFR
                     LRENGSLERIVENAPSPNGITLNNRNTQVYVAVTRSQQIWRLPLMPGGSLSKTGVAIQ
                     LSGGHAGPDGIEMDEADGIVVCHLGVGIWRFDANCLPTRLAHAGSQHRLLTNIAFGGP
                     DRKTLYVTDSLNGDIVTAEMPVAGKKMFAHR"
     gene            3148..4101
                     /locus_tag="VI319_16390"
     CDS             3148..4101
                     /locus_tag="VI319_16390"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865474.1"
                     /translation="MKLLIALIAAIAAVAAHAQQYPTKPVRVLIGYAAGSSTDIVGRV
                     MADRLSAHWKQSVIVENRGGAAGNLAGEAAAKAAPDGYTLLFAQNGLAISAAALANLP
                     YKPESELLPLAPVAATPHILIVRPDFPAKTLRDLIALAKKDPGKLSYASSGVGNSDHL
                     CGVLFNLQAGIEAVHVPYKGGAPAVQDLLPGRVDYYFAGMPVGLPLVKSGRARTFGVT
                     SRKRFEGAPEIPTIAEQGLPDYEATLWQGFFAPAAIDRGLAARIAKEIESVVAQPDTQ
                     EKLHKAGVSVFQDNQANFDRYFHGEIAKWRDVVARTHLKLD"
     gene            4106..4705
                     /locus_tag="VI319_16395"
     CDS             4106..4705
                     /locus_tag="VI319_16395"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865475.1"
                     /translation="MKILAALLLAALPLAAAGQELGPEELVRKVTSEVLDAIQHDKQL
                     QAGDRKKALALAEEKILPLVDFREAARLAVGRPWNTANPDQQERITREFRTLLVRIYS
                     SAIGVYRGQTMRVQPVKMAPGASQVTVRNQYLSPGRPPTSVEYAMHKTPEGWKIYDIT
                     VEGVSLVLTYRGEFEQVVREGGIEGLIQRLAERNREPGR"
     gene            4702..>5131
                     /locus_tag="VI319_16400"
     CDS             4702..>5131
                     /locus_tag="VI319_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6865476.1"
                     /translation="MKAESQTGLRRRLEQLERDGLMRKRRVVRGARGAWLDVDGRRIL
                     SFCSNDYLGLASHPDIVAAAQRALAESGVGAGASALISGHDARHELLEGRLAELVGAP
                     RALCFSTGYMANLGIVAAMTGRGDTVFSDELNHASLVDAAR"
BASE COUNT          853 a         1796 c         1699 g          783 t
ORIGIN      
        1 atcgcgtcgg gcagcaggtc cttgaactgc ggcgagcccg ccttctgcag cagcttctcg
       61 cggtgcagcg actcgagctc gggaccgttg gtctcgatca cgtcgacgtc gaagcggccc
      121 gcgcgcgcct cggtgaccga gcgctgcacc accttctccg agcccgcgcg ccagtagctg
      181 accttgacgc cgtacttctt ctggaacgcc gccgcgatcg cggtcaggtc ctcgacgacg
      241 agcgacgagt acagcgtcac ctggcgctcc ttcttcgcgg cctgggcgag cttctgctcg
      301 cggtcgggtc ccgcgtactg cgcgaggccc gcgatgtcgg cgctctgcgc gcgcaaggga
      361 ccggcagcga gcgcgcacag cgccgccggc agcaaccacc ggtagatgct cttcatgtct
      421 ctttcctctt cgtttcctcg tttgcgattt atgttttttt tccgactact cgatgtcagc
      481 cggacgcgtc cggcgtcaat cccgcctgct ggatgccgtg gacgcaggcc tcctcgtcct
      541 cctcgccggt gccaccgctc gcgccggccg cgccgaggat ctcgccctgc gcgtccttga
      601 tgagcacgcc gcccgtttgc ggcaggaact tgcccttcgc cgtcgccgca agcgtgacga
      661 agaaattggg attgtccttg gcgcgcttga gcagcgcgcg gctggaggcg cccatcgcca
      721 ccgacgccca cgctttgccc agggccacgt cgaagcggaa catgctggcg ccgtcctcgc
      781 gctgcgccgc cttcacgttg cccgcgtcgt cgagcaccac cacggcgagg ggcttgatct
      841 tcatctgccg cgcccgctcg agcgccttgt cgacgatgag gttggcttgc ttcagggtca
      901 gcttcgacat tcctcgactc ctccatgttg tcagggctcc cattataggg agcggcccac
      961 tatgacgccg tgcgcgccgg tccgctacaa tcggcctctc tcggacccct caggaggaga
     1021 agacatgaac cgcaccgtcg cagcagtgct gggcgcgctc gcgtgcttcg ccgcgtccgg
     1081 cgcgctcgcg cagcagcccg tccggatcgg attgatcatg gagtactcgg gcccgttcgc
     1141 agacgccgcg gcccagatgg acaacggcgt gaagctgtac atgaagcagc acggcgacac
     1201 cgtcggcgga aagaagatcg agatcatccg ccgcgacacc ggcggccccg cgccggacgt
     1261 gtccaagcgc cacgcgcagg agctggtcgt gcgcgaccac gtcgacgtcc tcgccggctt
     1321 cgtgctgacg cccaacgcgc tcgccgcggc cgacgtctcc gccgaggcga agaagttcac
     1381 cgtggtgatg aacgccgcga cctcgatcat caccaccaag tcgccctacc tcgtgcggac
     1441 atcgctcacc atcccgcagg tggtcgagcc gctcggttcc tgggcctacc gcaacggcgt
     1501 gcgcaaggcg tatacgctcg tctccgacta cggcccgggg atcgacgcag agggtgcgtt
     1561 ccagcgcgcg ttcaaggccg ccggcgggga gatctccggc tcggcgcgca cgcccctcgc
     1621 gaatcccgac ttcttcccgt tcgtgcagcg cgcgaaagac ctgaaccccg aggcgatgtt
     1681 catcttcgtc cccggcggcg cgcagcccgc ggcgctcggc aaggccttcg tcgacctcgg
     1741 catggattcc aagaagacga agatcatggg ccagggcgag atcaccgagg agaacgcgat
     1801 caaggtcatg ggcgacgccg cgctcggcct gatcaccgcg ttccactacg actacaacca
     1861 caagtcggcg atgaacgcga agttcgtcaa ggagtacaac gccgccttcg gccgcaatcc
     1921 cgacttcttc accgtcggcg gctacgacgg catgcacctg atctacgagg cgctgaagaa
     1981 agccaaaggc aagaccgacg gcgactcgct ggtcgcggcg gcgaagggca tgacgtggga
     2041 aagcccgcgc ggcccgatgt cgatcgaccc cgagacgcgc gacgtcgtgc agacggtcta
     2101 catccgccgc gtcgagaagg tcggcggcaa gctcgtcaac gtcgagatcg acaagatcga
     2161 gaacgtgaag gacccggtga aggaccggat gaagaagtag cccgccttcg tccatgttca
     2221 atccgttcca ggagatcgag cgcgtcgagg tgaagctctt cacctcgatg cccgcgaagt
     2281 tccggaagaa gggccgcacc gcctggtccg atcccaaccg cggcggcgca gaggtcgaca
     2341 gcttcctcga ggggccgtcg ttcgaccgcc acggcaacct gtactgcgtc gacatcccgt
     2401 tcgggcgcgt cttccgcatc gacccgaagg gcgaatggga tctcgtcgtc cagtacgacg
     2461 gctggccgaa cgggctcaag atccacaagg acgggcgcat cttcatcgcc gactacaagc
     2521 gcgggctgat gggtctcgat gcgaagagcg gccgcatcga gcccctggtc gagacggcgt
     2581 tcagcgagag cttcaagggc ctgaacgacc tgcacttcgc cgacaacggc gacctgtact
     2641 tcaccgacca ggggcagaca ggcatcgcgg atccgacggg gcgggtgttt cgcctccggg
     2701 agaacggatc gctcgagcgc atcgtcgaga acgccccttc gccgaacggc atcacgctca
     2761 acaaccgcaa cacgcaggtc tacgtcgcgg tcacgcgctc gcagcagatc tggcgcctgc
     2821 cgctgatgcc gggcggatcg ctgtcgaaga cgggcgtcgc gatccagctc tcgggcggtc
     2881 acgcgggtcc cgacggcatc gagatggacg aggcggacgg catcgtcgtg tgccacctgg
     2941 gcgtcggcat ctggcgcttc gacgcgaact gcctgccgac gcggctcgcg cacgccggca
     3001 gtcagcatcg cctcctgacc aacatcgcct tcggcggccc cgaccggaag acgctgtacg
     3061 tgaccgactc gctcaacggc gacatcgtca ccgccgagat gccggtcgcc ggcaagaaga
     3121 tgttcgcgca tcgctgaaag aggaatcatg aagctcctta ttgcactgat tgccgccatc
     3181 gccgcggttg ccgcacacgc gcagcagtac cccacgaagc cggtgcgcgt gctgatcggc
     3241 tatgccgcgg gaagctcgac cgacatcgtc ggccgcgtga tggccgaccg cctctcggcg
     3301 cactggaagc agagcgtcat cgtcgagaac cgcggcggcg ccgccggcaa cctcgcgggc
     3361 gaggcggcgg cgaaggccgc gcccgacggg tacacgctgc tgttcgcgca gaacggcctg
     3421 gcgatcagcg ccgccgcgct cgccaatctg ccgtacaagc cggagagcga gctgctgccg
     3481 ctcgcgcccg tcgcggcgac gccgcacatc ctcatcgtgc gtccggattt cccggcgaag
     3541 acgctgcgcg acctgatcgc gctcgcgaaa aaggacccgg ggaagctcag ctacgcctct
     3601 tccggcgtgg gcaactccga ccatctgtgc ggcgtgctgt tcaatctgca ggcgggcatc
     3661 gaggcggtgc acgtcccgta caagggcggc gcgcccgcgg tgcaggacct gctgcccggc
     3721 cgcgtcgact actacttcgc cgggatgccg gtcgggctgc cgctggtgaa atccgggcgc
     3781 gcgcgcacgt tcggcgtgac gagcaggaag cgcttcgagg gcgcgcccga gatcccgacc
     3841 atcgccgagc agggcctgcc cgattacgag gcgacgctgt ggcagggctt cttcgcgccc
     3901 gccgcgatcg accgcgggct cgccgcgcgc atcgcaaagg agatcgaatc cgtcgtcgcc
     3961 cagcccgata cgcaggagaa gctccacaag gcgggcgtct ccgtgttcca ggacaaccag
     4021 gcgaacttcg accgctattt ccacggcgag atcgcgaagt ggcgcgacgt cgtcgccagg
     4081 acccatctca agctggatta ggccgatgaa gattctcgct gcactgctgc tcgccgcgct
     4141 gcctctcgcc gccgcgggcc aggagctcgg tcccgaggag ctcgtgcgca aggtcacctc
     4201 cgaggtgctc gacgcgatcc agcacgacaa gcagctccag gccggggacc gcaagaaggc
     4261 gctcgcgctg gcggaggaga agatcctgcc gctggtcgat ttccgcgagg cggcccggct
     4321 cgccgtcggc cgcccgtgga acacggcgaa cccggaccag caggagcgca tcacgcgcga
     4381 gttccgtacc ctgctcgtcc ggatctactc gagcgcgatc ggcgtctacc gcgggcagac
     4441 gatgcgggtg cagccggtga agatggcgcc cggcgcgtcc caggtgaccg tgcgcaacca
     4501 gtacctgagc ccgggccggc cgccgacctc cgtcgagtac gcgatgcaca agacgccgga
     4561 gggctggaag atctacgaca tcacggtcga aggcgtgagc ctggtcctca cctatcgcgg
     4621 cgagttcgag caggtcgtgc gcgaaggcgg catcgaaggc ctgatccagc ggctggccga
     4681 aaggaatcgg gaacccggca ggtgaaggcg gaatcgcaga ccggtctccg gcgccggctc
     4741 gagcagctgg agcgggacgg gctgatgcgc aagcgccggg tcgtgcgggg cgcgcgcggt
     4801 gcctggctcg acgtcgacgg gcggcgcatc ctctccttct gcagcaacga ctatctcggc
     4861 ctcgcgtcgc atccggacat cgtcgccgcc gcgcagcgcg cgctcgccga atcgggcgtc
     4921 ggcgcgggag cctcggcgct gatcagcggc cacgacgcgc ggcacgagct gctcgagggg
     4981 cgcctcgccg agctcgtcgg cgcgccgcgc gcgctctgct tctccaccgg gtacatggcg
     5041 aacctcggca tcgtcgccgc gatgacgggg cgcggcgaca cggtgttctc cgacgagctg
     5101 aaccacgcga gcctcgtcga cgctgcgcgc c
//
LOCUS       DATDMW010000393         2866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_490227, whole genome shotgun sequence.
ACCESSION   DATDMW010000393 DATDMW010000000
VERSION     DATDMW010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490227"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VI319_16405"
     CDS             complement(<1..414)
                     /locus_tag="VI319_16405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6865477.1"
                     /translation="MNRLRIVHTESSCGWGGQELRIISESQGLIERGHDVSLLAPAES
                     RIYAEARKRGIPSRALPIARKGLRGLLALRRLLAAGRPDVVNTHSSTDTWLAALALAT
                     LPAAPPLVRTRHISAPIPPNAGSRWLYRRATAHVVT"
     gene            complement(411..2183)
                     /gene="msbA"
                     /locus_tag="VI319_16410"
     CDS             complement(411..2183)
                     /gene="msbA"
                     /locus_tag="VI319_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEY6865478.1"
                     /translation="MAQASTQRTSAEVYGRLLKYVKPYWHLFGVAILAMIVQAATEPI
                     LPALLKPLLDGSFVKRDLSNLHLIPIAIVGLFIVRGAFSYAGDYALSWVANKVVLDLR
                     NAMFGRLLTLPTRYYDDQSSGALLSKVAYDVTGVTGAATSVLTVLVRDTLTVALLLAW
                     LLWLNWKLTLVTLVIIPGIALVVKFFSKRMRRMSRESLRAMGDVTHVLEETIECHKVV
                     KIFGGQDYEARRFLGANQKLRGYAMRQTIAAAATVPIVQLFAAVAIAIIITLAVQQSA
                     TNQLSVGGFVSFITAMLMLLAPLKRLTEVNSPLQRGLASAESVFQLLDEQAEEDAGKR
                     SIERASGRLEFDRVSFAYPGSGREALDGVSIRIEPGETVALVGPSGGGKTTFVNLIAR
                     FYEPTAGRILVDGVDLREIALGSLRANLALVSQDVTLFNDTVAANIAYGRMGGANRAT
                     IEAAARAAHALDFIREMPQGFETLIGENGVRLSGGQRQRLAIARAVLKDAPILLLDEA
                     TSALDSESERHVQAALDELMRGRTTIVIAHRLSTIEHADRIVVLQRGRIAEVGPHAEL
                     IARDGLYARLHRIQFALEREGAPA"
     gene            complement(2251..>2866)
                     /locus_tag="VI319_16415"
     CDS             complement(2251..>2866)
                     /locus_tag="VI319_16415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6865479.1"
                     /translation="VVYNGVDLEAFHPRLRESFGARARTELGIPPDAMTWLFVGSGFE
                     RKGVSRLVRAFRDGADPGAHLIVVGSDRRELRARAEARDLGARVHFLGGRDDVRPWYG
                     AADAFVLPTLYDPFPNAALEAMACALPVVTTLQCGAAELVRADENGWVCDALDAVSLA
                     RFMGALDRTRARTMGERARATAEGYGLDAMAQKLLALYRALGPR"
BASE COUNT          437 a          993 c         1038 g          398 t
ORIGIN      
        1 cgtgacgacg tgcgcggtcg cgcgccggta gagccagcgg ctgccggcgt tgggcgggat
       61 cggcgccgag atgtggcgcg tgcgcaccag cggcggcgcg gcgggcagcg tcgcgagcgc
      121 gagcgccgcg agccaggtgt cggtcgagct gtgggtattg acgacgtcgg gacggccggc
      181 cgcgagaagc cgccgcagcg cgagcagccc gcgcaggccc ttgcgcgcga tgggcagcgc
      241 ccgcgagggg atgccgcgct tccgcgcctc ggcgtagatg cgcgattccg cgggcgcgag
      301 caggctcacg tcgtggccgc gctcgatcag gccctgcgac tccgagatga tccgcagttc
      361 ctgtcctccc cagccgcacg acgattcggt atggacgatc ctcaagcggt tcaagccgga
      421 gcgccctctc gctcgagcgc gaactggatg cggtgcaggc gcgcgtacag gccgtcgcgc
      481 gcgatcagct cggcatgcgg cccgacctcg gcaatgcgcc cgcgctgcag cacgacgatg
      541 cggtcggcgt gctcgatggt cgagagccgg tgcgcgatca cgatcgtggt gcggccgcgc
      601 atcagctcgt cgagcgcggc ctgcacgtga cgctcggact cgctgtcgag cgccgaggtc
      661 gcctcgtcga ggagcaggat cggcgcgtcc ttgaggaccg cgcgcgcgat cgcgaggcgc
      721 tggcgctggc cgcccgagag ccgcacgccg ttctcgccga tcagcgtctc gaacccctgc
      781 ggcatctcgc ggatgaaatc gagcgcgtgc gcggcgcgcg cggccgcctc gatcgtcgct
      841 cggttcgcgc cgcccatccg gccgtaggcg atgttggctg cgaccgtgtc gttgaagagc
      901 gtgacgtcct gcgacacgag cgcgaggttg gcgcgaaggc tgccgagcgc gatctcgcgc
      961 aggtcgacgc cgtcgaccag gatgcggccg gcggtcggct cgtagaacct cgcgatgagg
     1021 ttgacgaagg tggtcttgcc cccgcccgaa ggcccgacca gcgccacggt ctcgcccggc
     1081 tcgatgcgaa tgctcacgcc gtccagcgcc tcgcggccgc tcccgggata cgcgaacgac
     1141 acgcggtcga actcgagccg gccgctcgcg cgctcgatcg agcgcttgcc ggcatcctcc
     1201 tcggcctgct cgtcgagcag ctggaagacg ctctcggccg aggcgaggcc gcgctgcagc
     1261 ggcgagttca cctcggtcag gcgcttgagc ggcgcgagca gcatcagcat cgcggtgatg
     1321 aacgagacga agcccccgac cgagagctga ttcgtcgccg actgctgcac ggcgagcgtg
     1381 atgatgatgg caatggcgac cgcggcgaac agctgcacga tcggcacggt cgccgccgcg
     1441 gcgatggtct ggcgcatggc gtagccgcgc agcttctggt tcgcgccgag gaagcggcgc
     1501 gcctcgtagt cctgcccgcc gaagatcttc accaccttgt ggcactcgat cgtctcctcg
     1561 agcacgtggg tgacgtcgcc catcgcgcgc agcgactcgc ggctcatgcg gcgcatgcgc
     1621 ttggagaaga acttcacgac gagcgcgatg ccggggatga tcacgagcgt gaccagcgtc
     1681 agcttccagt tcagccacag cagccacgcg agcagcagcg cgacggtgag cgtgtcgcgc
     1741 accagcaccg tcagcacgct ggtcgccgcg ccggtgacgc ccgtgacgtc gtacgccacc
     1801 ttggagagca gcgccccaga gctctggtcg tcgtagtagc gcgtcggcag cgtcagcagc
     1861 cggccgaaca tggcgttccg gaggtcgagc acgaccttgt tcgcgaccca cgagagcgcg
     1921 tagtcgccgg cgtagctgaa cgcgccgcgc acgatgaaaa ggcccacgat cgcgatcggg
     1981 atcaagtgga ggttcgacag gtcgcgcttg acgaagctgc cgtcgagcag cggcttgagc
     2041 agcgcgggca ggatcggctc ggtcgccgcc tgcacgatca tcgcgaggat ggcgacgccg
     2101 aacagatgcc agtacggctt cacgtacttc aggagccgtc cgtacacttc ggcggaagtg
     2161 cgctgcgtcg aggcttgcgc cattcgcgtc ccttacggaa aacgttgaat tataaccgct
     2221 ttgaacgcct ccgccgaccg aagcccgcgc tcaccggggg ccgagcgcgc ggtagagcgc
     2281 gagcagcttc tgcgccatgg cgtcgagccc atagccctcg gcggtcgcgc gtgcgcgctc
     2341 gcccatggtg cgggcgcggg tgcgatccag cgcccccatg aatctcgcga gagacaccgc
     2401 atcgagcgcg tcgcagaccc agccgttttc gtcggcgcgc acgagttcgg cggcgccgca
     2461 ctggagcgtg gtcacgaccg gcagcgcgca cgccatcgcc tcgagcgcgg cgttcggaaa
     2521 cggatcgtag agcgtcggca gcacgaaggc gtcggccgcg ccgtaccatg ggcgcacgtc
     2581 gtcgcgcccg ccgaggaagt gcacgcgggc gccgaggtcg cgcgcctcgg cgcgtgcgcg
     2641 cagctcgcgc cggtccgagc cgacgacgat caggtgtgcg cccggatccg cgccgtcgcg
     2701 gaacgcgcgc accagccgcg acacgccctt gcgctcgaag cccgagccga cgaacagcca
     2761 ggtcatcgcg tcgggcggga tgccgagctc ggtgcgcgcg cgcgcgccga acgactcgcg
     2821 cagccgcggg tggaaggcct cgagatcgac gccgttgtag acgacg
//
LOCUS       DATDMW010000394         3573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_117762, whole genome shotgun sequence.
ACCESSION   DATDMW010000394 DATDMW010000000
VERSION     DATDMW010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117762"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..349
                     /locus_tag="VI319_16420"
                     /pseudo
     CDS             149..349
                     /locus_tag="VI319_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            334..3252
                     /locus_tag="VI319_16425"
     CDS             334..3252
                     /locus_tag="VI319_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6865480.1"
                     /translation="MLQRLIDTSALRPAQGGFRDARRLEAELRARIEGEVRFDDGSRA
                     LYATDASNYRQVPIGVVIPRTIDDVIATVAACRAHGAPVLARGGGTSLCGQCCNVAVV
                     MDFSKYLNRILELDPERRIARVQPGVVLDRLREAAEKHHLTFGPDPSTHDHNTLGGMI
                     GNNSCGVHSLMAGETVHNVIELDVLTYDGERMTIGETPPERLDAILAAGGRRAAIYRG
                     LRELASRHASEIRRRYPHIPRRVSGYNLEALLPEHGFDVARAIVGSEGTCAVILGATL
                     RLVPSPPARTLLVLGYPSVYEAGDHVPEIVRFGPIGLEGIDDRLIQDMKAIKLHPENL
                     KLLPDGGGWLIAEFGGATREESDARARRAMDALKKTANPPSMKLYDDPREEKLIWKVR
                     ESGLGATAHVPNKKITWEGWEDSAVPIDRLGEYLRKLRALFEKYGYDCDLYGHFGQGC
                     VHTRIDFDLETAEGIRIFRAFLHDAAHLVVSLGGSISGEHGDGQSKAELLPIMFGDEL
                     MQAFRDFKRIWDPEGRMNPGKVVDAFRADENLRLGARFNPVVPETHFRFPEDRGDFSR
                     TVLRCVGVGECRKQSGTMCPSYMATGEEMHSTRGRARLLFEMMRGDALRDGWKSDAVR
                     ESLDLCLSCKGCKGECPVSVDMASYKAEFMAHHYEGRLRPRGAYAFGLIDRWARVASA
                     APGLVNWLARTPPFVDLARALAGMPEERAIPRFARETFRDWYRKRPKRGSGGAKAILW
                     ADTFNNYFHPDTARAALAVLEGAGVDVRVPGRHLCCGRPLYEFGFLERARRYLTEILD
                     ALAAEIDAGIPIVVLEPACASVFRDELLNLFPRNERAKRLAAQTFLLTEFLEQRVDGW
                     APRALRRQALVHAHCHHKSILGTEAEAAVLAKLGLDFTMLDSGCCGMAGSFGFDARKY
                     PVSIAAGERVLLPAVRAAAADTLIVAEGFSCREQIAQCTGRRALHPAEVIAEAIAAPA
                     "
     gene            complement(3249..>3573)
                     /locus_tag="VI319_16430"
     CDS             complement(3249..>3573)
                     /locus_tag="VI319_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865481.1"
                     /translation="RDAVRKIAAWFAADQGHEPQRVSLLRRPGDPRFYRAVYARLVSG
                     QPLAADAARSLAERRAQAVIGYLRSAGADPARLAAGEMQIAHGEGDAVPAELALQAAG
                     SAPAG"
BASE COUNT          507 a         1283 c         1297 g          486 t
ORIGIN      
        1 gtggcgatcg tattgattac gtcctcctcc gtgcggggga ccaccacgcc gatcggcacc
       61 tggcggtagt tcgaggcgtc ggtcgagtag agggcgcggc tgccgtcgtc gaagcggacc
      121 tcgccctcga tcgcggcggc gaggtcgcgc gcccgcgctg cacgcgccgc tgtgctgcgc
      181 ggcgcggcgc gcggtgcacc tcgagtggtt ccacgatcac gcgcgcatcg agtcgatgct
      241 cttcgacggc gcgccccggc ctgcgcgcgg cgaggtcgcg cccgaccgct cgcggccggg
      301 gctcggcctc gagctcaagg agaaggacgc cgaatgctac agcgcctgat cgacacgagc
      361 gcgctgcgcc cggcgcaggg cggcttccgc gacgcgcggc gcctggaagc cgagctgcgc
      421 gcccgtatcg aaggcgaggt gcgcttcgac gacggcagcc gcgcgctgta cgcgaccgac
      481 gcctcgaact accgccaggt gccgatcggc gtcgtcatcc cgaggacgat cgacgacgtg
      541 atcgcgacgg tcgccgcgtg ccgcgcgcac ggcgcgccgg tgctcgcgcg cggcggcggc
      601 acgagcctgt gcgggcagtg ctgcaacgtc gcggtggtga tggacttctc gaagtacctg
      661 aaccgcatcc tcgagctcga cccggagcgc cgcatcgcgc gcgtgcagcc cggcgtcgtg
      721 ctcgaccggc tgcgcgaggc ggcggagaag caccatctca ctttcggccc cgacccgtcg
      781 acgcacgacc acaacacgct cggcgggatg atcggcaaca actcgtgcgg cgtgcactcg
      841 ctcatggccg gcgagacggt tcacaacgtg atcgagctcg acgtcctcac ctacgacggc
      901 gagcggatga cgatcggcga gacgccgccc gagcggctcg acgcgatcct cgccgcgggc
      961 ggccggcgcg cggcgatcta ccgcggcctg cgcgagctcg cctcgcgcca cgccagcgag
     1021 atccgccgcc gctatcccca catcccgagg cgcgtctcgg gctacaacct cgaggcgctg
     1081 ctgcccgagc acggcttcga cgtggcgcgc gcgatcgtcg gctccgaggg cacctgcgcc
     1141 gtgatcctcg gcgcgacgct gcgcctggtg ccgagcccgc ccgcgcgcac gctgctcgtg
     1201 ctcggctatc cgagcgtcta cgaggcgggc gaccacgtgc ccgagatcgt gcgcttcggc
     1261 ccgatcggcc tcgagggcat cgacgaccgg ctgatccagg acatgaaggc gatcaagctg
     1321 cacccggaga acctgaagct gctcccggac ggcggcggct ggctgatcgc ggagttcggc
     1381 ggtgcgacgc gcgaggagtc ggacgcaaga gcgcgccgcg cgatggacgc gctgaagaag
     1441 accgcgaatc cgccgtcgat gaagctctac gacgacccgc gcgaggagaa gctcatctgg
     1501 aaggtgcgcg agtccggact gggcgcgacc gcgcacgtgc cgaacaagaa gatcacgtgg
     1561 gaaggctggg aggactcggc ggtcccgatc gaccgcctcg gcgagtacct gcgcaagctc
     1621 cgcgcgctgt tcgagaagta cggctacgac tgcgacctct acggccactt cggccagggc
     1681 tgcgtgcaca cgcgcatcga cttcgatctc gagaccgcgg aaggcatccg catcttccgc
     1741 gctttcctgc acgacgcggc gcacctcgtg gtgagcctcg gcggctcgat ctcgggcgag
     1801 cacggcgacg gccagtcgaa ggccgagctg ctgccgatca tgttcggcga cgagctgatg
     1861 caggcgttcc gcgacttcaa gcgcatctgg gaccccgagg gccgcatgaa cccgggcaag
     1921 gtcgtcgacg ccttccgtgc cgacgagaac ctgcgcctcg gcgcgcgctt caaccccgtc
     1981 gtgcccgaga cgcacttccg cttccccgag gaccgcggcg atttctcgcg cacggtgctg
     2041 cgctgcgtcg gcgtcggcga gtgccggaag cagagcggga cgatgtgccc gagctacatg
     2101 gcgaccgggg aggagatgca ctcgacgcgc ggccgcgcgc gcctgctgtt cgagatgatg
     2161 cgcggcgacg cgctgcgcga cggctggaag tccgacgcgg tgcgcgagtc gctcgacctg
     2221 tgcctgtcgt gcaagggctg caagggcgag tgcccggtga gcgtcgacat ggcgagctac
     2281 aaagccgagt tcatggcgca ccactacgaa ggccgtctgc ggccgcgggg cgcgtacgca
     2341 ttcgggctga tcgatcgctg ggcgcgcgtg gcgtccgcgg cgcccggtct cgtcaactgg
     2401 ctcgcgcgca cgccgccctt cgtcgacctc gcgcgcgcgc tcgccggcat gcccgaggag
     2461 cgcgcgatcc cacgcttcgc gcgcgagacg ttccgcgact ggtatcggaa acgcccgaag
     2521 cgcggcagcg gcggcgcgaa ggcgatcctg tgggccgaca ccttcaacaa ctacttccac
     2581 ccggacacgg cgcgcgcggc gctcgccgtg ctcgagggcg ccggggtcga cgtgcgcgtg
     2641 cccgggcggc acctgtgctg cgggcggccg ctctacgagt tcgggtttct cgagcgcgcg
     2701 cgccgctatc tgaccgagat cctggacgcg ctcgcagccg agatcgacgc cgggatcccg
     2761 atcgtggtgc tcgagcccgc gtgcgcgtcg gtgttccgcg acgagctgct caacctattc
     2821 cccaggaacg agcgcgcgaa gcggctcgcg gcgcagacct tcctcctgac cgagttcctg
     2881 gagcagcgcg tcgatggctg ggcgccgcgc gcgctgcgaa gacaggcgtt ggtgcacgcg
     2941 cactgccacc acaagtcgat cctcgggacc gaggccgagg cggcggtgct cgcgaagctc
     3001 gggctcgact tcacgatgct cgactcgggc tgctgcggca tggcgggctc gttcggcttc
     3061 gacgcgcgca agtacccggt gtcgatcgcg gccggcgagc gcgtgctgct gcccgcggtg
     3121 cgcgcggccg ccgccgacac gctgatcgtc gccgaaggct tcagctgccg cgagcagatc
     3181 gcgcagtgca ccgggcgccg cgcgctgcat cccgcggagg tgatcgccga agcgatcgcg
     3241 gcgccggcct agcccgcggg agcgctgccg gcggcctgga gcgcgagctc cgcgggaacc
     3301 gcatcgcctt cgccgtgcgc gatctgcatc tccccggccg cgagacgcgc cgggtcggcc
     3361 ccggccgagc gcaggtagcc gatcaccgcc tgcgcgcgcc gctcggcgag cgacctcgcc
     3421 gcgtcggcgg cgagcggctg gcccgagacc aggcgcgcgt agacggcgcg gtagaatcgc
     3481 gggtcgccgg gccgccgcaa gaggctcacg cgctgcggct cgtgtccctg gtcggcggcg
     3541 aaccacgccg cgatcttgcg caccgcatcg cgg
//
LOCUS       DATDMW010000395        10370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_366086, whole genome shotgun sequence.
ACCESSION   DATDMW010000395 DATDMW010000000
VERSION     DATDMW010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366086"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="VI319_16435"
     CDS             complement(<1..1638)
                     /locus_tag="VI319_16435"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY6865482.1"
                     /translation="MKPLLAALLVCTAAAHAGPGDRTADTIFVNGRIVTLDAKSSLRK
                     AMAVKDGRIVALSDAAGAPDLQGWEGRSTRIVDLKGRTVVPGLIDSHIHAIRAALSFS
                     TEVNWIGAKSLDEALGRLRAAAAGARPGDWLIVAGGWTPEQFREKRRPTQAELAAAAP
                     DHPVYVQLFYSWAILTPKGLAALGLAEDKDVPAGGKLERDAAGKLTGGVTGSPGTIIA
                     LFAKLPKPTPEQQVEGTKKFFAELNRLGLTGVIDPGGFGMYPSAYQALFKVWREGGLT
                     LRVAYSYFAQDRGKELDDFKAMTQLMPMGLGDDWLRFNGIGENVFWGAYNVDAPDDAA
                     RERLVEILRWAAQNRMSVNMHWNNDRSVGVLLDAFERVDRETPIAPLRWAIAHLNDAS
                     SASLERMKALGVAWAMQDAMYYEGEQHLKEKGEAAVRRMPPLATAKKIGVRIGAGTDA
                     HRVASYNPWAALQWMQDGRTVGGAALRDASETPSREDALRMSTQGSAWLAHAERERGS
                     LEVGKLADFAVLSKDYLSVPVAEIGAIESVMTVVGGRI"
     gene            complement(1649..2260)
                     /locus_tag="VI319_16440"
     CDS             complement(1649..2260)
                     /locus_tag="VI319_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865483.1"
                     /translation="MRITRRTALTLVLAAAAPLFGTGALAQNIDWSRWYAGAGLGLGI
                     NAHYRQDGRTLNFDEGMPGATDKSVLGAYRVEGGVQLDPKTLVGLSVSGVGKTAKING
                     NDANTNIDNVLATVTWLPAERGFFLRAGLGYSGMVVDDGVDKRHAGGLGVLAGLGWMW
                     NVVSEHYVTLSVDQSFQFYHSNNDHRPTRSEFSAAYLGYMYRH"
     gene            complement(2284..2760)
                     /locus_tag="VI319_16445"
     CDS             complement(2284..2760)
                     /locus_tag="VI319_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865484.1"
                     /translation="MKTSMLVVAVLSLSLAASYAFAQGKGGGGGHGGPPGVGGSPFPS
                     PTPTPSPAPSSAPAPGASSTPILSTPIGGGTGGSTVGAPAPAADPLSGPATPMAPSAG
                     SAPPVDRFRELHGHAHAHLCKAKGCEQYCDGRERAVGAGKRDTFLLQCRTYCMEKC"
     gene            2878..3459
                     /locus_tag="VI319_16450"
     CDS             2878..3459
                     /locus_tag="VI319_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY6865485.1"
                     /translation="MNKVRLEAFSDGVIAVIITILVLEMKVPHDADPGALVPLAPVFA
                     SYVLSFVFVGIYWANHHHLFHAVHRVKGSVLWANLHLLFWLSLVPFVTGWMDENHFAA
                     LPVAAYGVVLLGAAIAYTLLTRTLLTLHDRESLLARALGRDAKGWLSLALYLCAVPLA
                     FVQPMISCGIYVLVAAIWLCPDPRFEKLLGEKA"
     gene            complement(3456..4403)
                     /locus_tag="VI319_16455"
     CDS             complement(3456..4403)
                     /locus_tag="VI319_16455"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6865486.1"
                     /translation="MKLLDLASWRLGWTALGPGSHGARLSSHFQAIFSAAERRPVGYE
                     ALIRATREDGLAIEPMQLLTSVPEGAARAELDRRCRTVQIEKFMRLGDGRSSLFLNLD
                     PCRVIEPRFAGVFADVLESCGLGAERVAIELTEWPGPCEERLADAAAFYREIGCRIVI
                     DDFGAGQSNFERVWRLQPDIVKIDREMTRRLSTNTVARRMLAGIVDVLHDGGASVCVE
                     GIETEDQALCAIDAGADLLQGFYFSRPGEALVAPEASRDVFDHLRDVSARRHAAALAE
                     ASPRSHSVARRSRNLLRHARVTGNVIRLRRRLAVGEDAA"
     gene            complement(4580..6058)
                     /locus_tag="VI319_16460"
     CDS             complement(4580..6058)
                     /locus_tag="VI319_16460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6865487.1"
                     /translation="MSSDAAQSAGAPGRGAADAAAGDDRPDLVRRLKVSLVAAAVVPT
                     LFFAGAAWIFAARHPEATAPEWRGELVALALVALAIAAGLVVLAWVALQRAKRAFVHA
                     QTASAESARRMRAEHALLQQQKLEALGRLTGGVAHDFNNLLMIINNNAYLLRRGDPTA
                     PIAPRIAAIERAVESGTKLTRQLLAFSRRQAVNPELIRLQDELPQLVELMKPALGSRI
                     SVKQDVDPATSPVRVDRAGLELALLNLAVNAGDAMPDRGELYATVRNAKPGEAPGLEG
                     DFVVLSVADTGTGIPEDVQQRVFEPFFSTKPIGKGTGLGLSQVYGFCQQSGGTVTIES
                     QEGVGTTVRLYLEASGEQVKEKDAEPQSFQGEALRHRVLIVEDNDDVAETTRQLLEFL
                     GCEVRRVESGNAAVLRLERDARNFDLVLTDIVMPGGLSGIEVLRYVRDRHPHLPVVLM
                     TGYSNELQKVGSDEVVLLKPFSPHELADALRKALARREGATT"
     gene            6230..6751
                     /locus_tag="VI319_16465"
     CDS             6230..6751
                     /locus_tag="VI319_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865488.1"
                     /translation="MSRTIALALAALLAGCASTTPTEEGMIPAFTVRGVRGPVHPEAV
                     AIEVKDGGRIPGAAFRQALADAVTMSRVFSRVAPSGPYVLSVVVNRLEVPESRFNINL
                     APTVRMEAAWTLRRAGTRAALWQATVRSESTSQPSDTLDGKARLRMAIEGAARENIAS
                     GLARLAKVSLAPT"
     gene            complement(6748..7710)
                     /locus_tag="VI319_16470"
     CDS             complement(6748..7710)
                     /locus_tag="VI319_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865489.1"
                     /translation="MTQIRVFVSALLGLVACGAFAQGWPSRPVHVVVPYPAGGPNDII
                     VRMVGKRLGETLGQPIVVENRPGAGGNIGTDSVAKSAPDGYTLVSVGPGALIINPLLG
                     KVPYDTMRDFAPVTIMAIAPNALVAHPSFPAKSVKELIALAKQKPGAINYASGGSGST
                     PHLAAALFAVMAGVQLTHVPYKGTGPATADLVGGQVQIAFLGIPTVLPHIRSGKLRAL
                     AVTGTKRSPELPGVPTVAEAGVPGYELSPWYGLLAPAGTPSAVVTRLAAEATKIVHEP
                     AMREQLVAQGAEPAGGTPEEFARTLRSDAALWKKVVKEAGIRAD"
     gene            7764..8720
                     /locus_tag="VI319_16475"
     CDS             7764..8720
                     /locus_tag="VI319_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865490.1"
                     /translation="MKLRLTLLTFAFLSSSALAQYPNRPIHIVAPFPAGGGYDFLSRL
                     LAAEMTKTFRQPVVVENKSGANGNIGSDFVAKSPADGYTLLMGGNSPLALNVGLYPKL
                     PYDPAKDFDAISRVATQPNLLAANPGKLPVKSVAELVKYAKANPGKVTYGSNGNGSPQ
                     HLAAELLKRMAGIDLVHVPYKGAAPTAQALLAGEVAVAFNIILLPLPYVQSGRLNGLA
                     VASSHRSPLAPSLPTMAELGYPIDIDTWYGLVAPAGTPKDAIAKLNAETVRIVGLPDL
                     KEKTQSQGIELGGSTPEQFAAFLRADIAKWSKAIREMNITID"
     gene            8729..9736
                     /locus_tag="VI319_16480"
     CDS             8729..9736
                     /locus_tag="VI319_16480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6865491.1"
                     /translation="MILDVFNHFMPKSIFDRLSQLVPGHIALTAFPELPTLWDVNARL
                     RMMDEFGELQHVLSLANPPIEMLGTPDKTPELARIANDGLAELCAKHPDRFPTFIASM
                     PMNNVEACVAEADRAIGQLGARGIQVFTNVLGKPLSAPEFRPLFRAMAKHDLPVWIHP
                     IRGPQFPDYATEKASENEIWFTFGWPYETTACVTRLIFSGIYDELPGLKIITHHMGGM
                     VPFYAGKIDLGFDQIFHGKTDDNPVARKAGLKKRPGDYYRMLYADTSVNGSAAAARCG
                     HAFFGTSHCVFATDAPFDAEQGRMLIRETIKAVDALEIPKAEKDQIFHGNARRLLKLS
                     S"
     gene            9802..>10370
                     /locus_tag="VI319_16485"
     CDS             9802..>10370
                     /locus_tag="VI319_16485"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6865492.1"
                     /translation="MAAHVAYPDRTPAGAIIAIMEIWGVNDTMRRHAQEFAEAGFVCL
                     VPDLFWRQEPGVELSDRNPEHVKKAFDLYYEFDYDLGVRDMEDTVAFLEKMPGCNGKV
                     GSVGYCLGGKLNYLMCCRGTRMECAVAYYGTYIEHNIREAKNLSKPFMLHMAMKDRWV
                     QAEVNDLIERRLSPNPLVTIHKYPEADHA"
BASE COUNT         1533 a         3680 c         3592 g         1565 t
ORIGIN      
        1 gatccgcccg ccgaccacgg tcatcaccga ttcgatcgcg ccgatctcgg cgaccgggac
       61 gctcaggtag tccttcgaga gcaccgcgaa gtcggcgagc ttgccgacct cgagcgagcc
      121 gcgctcgcgc tcggcgtgcg cgagccaggc actgccctgc gtggacatgc gcagcgcgtc
      181 ctcgcgcgag ggcgtctccg acgcgtctcg cagcgccgcg ccgcccacgg tcctgccgtc
      241 ctgcatccac tgcagcgcgg cccaggggtt gtagctcgcg actcggtgcg cgtcggtgcc
      301 cgcgccgatg cgcacgccga tcttcttcgc ggtcgcgagc ggcggcatgc gccgcacggc
      361 ggcctcgccc ttctccttca ggtgctgctc gccctcgtag tacatcgcgt cctgcatcgc
      421 ccacgcgacg ccgagcgcct tcatccgctc gagcgacgcg ctcgaggcgt cgttcaggtg
      481 cgcgatcgcc cagcgcagcg gcgcgatcgg cgtctcgcgg tcgacgcgct cgaaggcgtc
      541 gagcagcacg ccgaccgagc ggtcgttgtt ccagtgcatg ttcacgctca tgcggttctg
      601 cgccgcccag cgcaggatct cgaccaggcg ctcgcgcgcg gcgtcgtcgg gcgcgtcgac
      661 gttgtacgcg ccccagaaga cgttctcgcc gatgccgttg aagcgcagcc agtcgtcgcc
      721 cagccccatc ggcatgagct gcgtcatggc cttgaagtcg tccaattcct tcccgcggtc
      781 ctgcgcgaag tacgagtacg cgacgcgcag cgtcagcccg ccctcgcgcc agaccttgaa
      841 cagggcctgg tacgcggacg gatacatccc gaacccgcca ggatcgatga cgcccgtgag
      901 cccgagccgg ttcagctcgg cgaagaactt cttcgtgccc tcgacctgct gctcgggcgt
      961 cggcttcggc agcttcgcga agagcgcgat gatcgtgccc ggcgacccgg tgacgccgcc
     1021 cgtgagcttg cccgcggcgt cgcgctcgag cttgccgccc gcgggcacgt ccttgtcctc
     1081 ggcgaggccg agcgcggcga gacccttcgg cgtcaggatc gcccagctgt agaagagctg
     1141 cacgtagacg ggatggtcgg gcgccgcggc cgcgagctcc gcctgcgtcg gccggcgctt
     1201 ctcgcggaac tgctccggcg tccagccgcc cgcgacgatc agccagtcgc cgggccgcgc
     1261 gcctgccgcc gccgcgcgca gccggccgag cgcctcgtcg aggctcttcg cgccgatcca
     1321 gttcacttcg gtcgagaacg agagcgcggc gcggatcgcg tggatgtgcg agtcgatcag
     1381 ccccgggacg acggtgcggc ccttgaggtc gacgatgcgc gtgctccgcc cttcccagcc
     1441 ctgcaggtcg ggcgctcccg cggcgtcgga aagcgcgacg atgcgcccgt ccttcaccgc
     1501 catcgccttg cgcagcgagg acttcgcgtc cagcgtgacg atcctgccgt tcacgaagat
     1561 cgtgtcggcg gtccgatcgc ccggcccggc gtgggccgcg gcggtgcaga cgaggagcgc
     1621 ggccagcagc ggcttcacgg cgcgcgtcct aatgccggta catgtagccg aggtacgccg
     1681 cggagaattc cgagcgcgtc ggccggtggt cgttgttcga gtggtagaac tggaacgact
     1741 ggtcgaccga gagcgtcacg tagtgctcgg acacgacgtt ccacatccag cccaggccgg
     1801 cgaggacgcc gagcccgccg gcgtggcgct tgtcgacgcc gtcgtccacg accatgcccg
     1861 agtagccgag cccggcgcgc aggaagaatc cgcgctcggc cgggagccac gtcaccgtcg
     1921 cgaggacgtt gtcgatgttc gtgttcgcgt cgttgccgtt gatcttcgcg gtcttgccga
     1981 cgcccgagac cgagaggccg accagcgtct tcgggtcgag ctgcacgccg ccctcgacgc
     2041 ggtaggcgcc gagcaccgac ttgtcggtcg cgccgggcat cccttcgtcg aagttgagcg
     2101 tgcgcccgtc ctggcggtag tgcgcgttga tgccgaggcc gagcccggcg ccggcgtacc
     2161 accgcgacca gtcgatgttc tgggcgagcg cacctgttcc aaaaagcgga gcagccgcgg
     2221 caaggaccag cgtgagagcg gtgcgacgag tgatgcgcat gcgtttctcc ccggtgacgg
     2281 tgttcagcac ttctccatgc aatacgtgcg gcattgcagg aggaaagtgt cgcgcttgcc
     2341 ggcgccgacc gcgcgctcgc ggccgtcgca atactgctcg cagcccttgg ccttgcacag
     2401 gtgcgcgtgc gcgtggccgt gcagctcgcg gaagcggtcg accggcggcg cgctgcccgc
     2461 ggaaggcgcc atcggcgttg ccggcccgga cagcggatcg gccgccgggg ccggcgcgcc
     2521 gacggtcgat ccgccggtgc cgccgccgat cggggtcgac aggatcggtg tcgaggaagc
     2581 gccgggggcc ggagcgctcg aaggcgcggg cgacggcgtc ggcgtcggcg aggggaaggg
     2641 cgagccgccc actcccggag gaccgccatg ccctccacca ccgcccttgc cctgggcgaa
     2701 ggcgtacgaa gcggcgagcg acagcgacag gaccgcgacg acgagcatgg aggtcttcat
     2761 gggtctctct ttgtcccttt tattgtgccg tgatcttacg ccgtcggcgg ccgcttgggt
     2821 cgccgggcgg cggcgtcagc ccggcgtaag atgcggcgga attcacggag gcttctcatg
     2881 aacaaggtcc ggctggaagc gttcagcgac ggcgtcatcg ccgtcatcat caccatcctc
     2941 gtgctcgaga tgaaggtccc gcacgacgcc gacccgggcg cgctcgtgcc gctcgcgccg
     3001 gtgttcgcga gctacgtgct ctcgttcgtg ttcgtcggca tctactgggc caaccaccat
     3061 catctgttcc acgcggtgca ccgggtgaag ggctcggtcc tgtgggcgaa cctgcacctg
     3121 ctgttctggc tctcgctcgt gcccttcgtc accggctgga tggacgagaa ccacttcgcc
     3181 gcgctgccgg tcgcggccta cggcgtggtg ctgctcggcg cggcgatcgc ctacaccctc
     3241 ctcacgcgca cgctgctcac gctccacgac cgcgagtcgc tgctcgcgcg ggcgctcggg
     3301 cgcgacgcga agggctggct gtcgctcgcg ctgtacctgt gcgcggttcc gctcgcgttc
     3361 gtgcagccga tgatttcctg cgggatctac gtgctggtcg cggcgatctg gctgtgcccg
     3421 gacccgcgct tcgagaagct gctcggggag aaggcctagg cggcgtcctc gcccaccgcc
     3481 aggcggcggc gcagccggat gacgttgccg gtgacgcgcg cgtggcgcag caggttgcgc
     3541 gagcggcgcg cgaccgagtg cgatcggggc gacgcttcgg ccagcgcggc cgcgtggcgg
     3601 cgcgccgaga cgtcgcgcag gtgatcgaac acgtcgcggc tcgcctcggg cgcgacgagc
     3661 gcctcgccgg gacgcgagaa gtagaagccc tgcagcaggt cggcgccggc gtcgatcgcg
     3721 cacagcgcct ggtcctcggt ctcgatgccc tcgacgcaga cgctcgcgcc gccgtcgtgc
     3781 agcacgtcga cgatgcccgc gagcatgcgc cgggcgacgg tgttggtgga caggcgccgc
     3841 gtcatctcgc ggtcgatctt gacgatatcg ggctgcaggc gccagacgcg ctcgaagttc
     3901 gactgcccgg cgccgaagtc gtcgatcacg atgcggcatc cgatctcgcg gtagaaggcg
     3961 gccgcgtcgg cgaggcgctc ctcgcacggc ccgggccact cggtcagctc gatcgcgacg
     4021 cgctcggcgc cgagcccgca ggactcgagc acgtcggcga acacgccggc gaagcgcggc
     4081 tcgatcacgc ggcacgggtc gaggttgagg aacaggctcg agcgcccgtc gccgagcctc
     4141 atgaacttct cgatctgcac ggtgcggcag cggcggtcga gctcggcgcg cgccgcgccc
     4201 tcggggacgc tcgtgagcag ctgcatcggc tcgatcgcga ggccgtcttc ccgggtggcg
     4261 cggatcagcg cctcgtagcc cacggggcgg cgctcggccg cgctgaagat cgcctggaaa
     4321 tggctggaga ggcgcgcccc gtgcgagccg ggaccgaggg cggtccagcc cagacgccag
     4381 gatgctagat cgagcagctt cacggtcgct ctccgcgttc cttgttgcaa ggcaatggag
     4441 caacggccgt gccatgcgcc cggaagcgat ttgcgccgtg ttcagcccgg gccggcgtga
     4501 aatattgcct gttaacggcc cgggctcgtc gccggatggc gacagttgcg gcgagcggcc
     4561 gacagcgggg ccggcggagc taggtggtcg cgccctcgcg ccgggccagc gccttgcgca
     4621 gcgcgtcggc cagctcgtgg ggcgagaacg gcttgagcag cacgacctcg tcggagccga
     4681 ccttctgcag ctcgttcgag tagccggtca tgaggacgac cggcaggtgc gggtggcggt
     4741 cgcgcacgta gcgcagcacc tcgatgcccg agaggccgcc cggcatgacg atgtcggtga
     4801 gcacgaggtc gaaattgcgc gcgtcgcgct ccaggcgcag caccgcggcg ttgcccgact
     4861 cgacgcggcg cacctcgcag ccgagaaact cgagcagctg gcgcgtggtc tcggcgacgt
     4921 cgtcgttgtc ctcgacgatg agcacgcggt ggcgcagggc ctcgccctgg aagctttgcg
     4981 gctcggcgtc cttctccttc acctgctcgc cgctcgcctc gaggtacagg cgcaccgtcg
     5041 tgccgacgcc ctcctggctc tcgatcgtga ccgtgccgcc cgactgctgg cagaagccgt
     5101 acacctgcga caggcccagg cccgtgccct tgccgatcgg cttggtggag aagaacggct
     5161 cgaacacgcg ctgctgcacg tcctcgggga tgccggtgcc ggtgtccgcg accgacagca
     5221 cgacgaagtc gccttccagg cccggcgcct cgccgggctt cgcatttcgc accgtcgcgt
     5281 acagctcgcc gcggtcgggc atggcgtcgc cggcgttgac cgcgaggttg agcagcgcga
     5341 gctcgaggcc cgcgcggtcg acgcgcacgg gcgaggtggc cgggtcgacg tcctgcttca
     5401 ccgagatcct cgatccgaga gccggcttca tcagctcgac cagctgcggc agctcgtcct
     5461 ggagccggat cagctccggg ttcaccgcct ggcgccgtga gaacgcgagc agctggcgcg
     5521 tgagcttggt gcccgactcg accgcgcgct cgatcgccgc gatgcgcggg gcgatcggcg
     5581 cggtcggatc gccgcggcgg agcaggtagg cgttgttgtt gatgatcatg agcaggttgt
     5641 tgaagtcgtg cgccacgccg ccggtgagcc ggccgagcgc ctcgagcttc tgctgctgca
     5701 gcagcgcgtg ctcggcgcgc atgcgccgcg ccgattccgc gctcgccgtc tgggcgtgca
     5761 cgaacgcgcg cttcgcgcgc tgcagcgcga cccaggcgag gaccacgagg ccggcggcga
     5821 tcgcgagcgc gaccagggcc agcgcgacga gctcgccgcg ccattcgggc gctgtcgcct
     5881 ccgggtgacg cgccgcgaag atccatgccg cgccggcgaa gaacagcgtc gggacgacgg
     5941 ccgcggcgac gagcgagacc ttcaggcggc ggacgaggtc gggccgatcg tcgccggccg
     6001 cggcgtcggc ggcgccccgg ccgggcgcgc ccgcgctctg ggccgcgtca ctgctcaata
     6061 tgcatccccc ggtttttgca cgctgtcttt gggcgccgtt tggaccgaaa agttcggcca
     6121 gccgggcgca agcatacccg ctttggggga aggaagtctc ccgccccgcc gcacgggtgt
     6181 catgttcggg gcgtaaacaa aggccgacac gtccccgagg agaacaacca tgtcgcgcac
     6241 gatcgccctc gccctcgccg ccctgctcgc cggctgcgcc agcaccacgc cgaccgagga
     6301 aggcatgatc ccggccttca ccgtgcgcgg cgtgcgcggg ccggtgcatc ccgaagcggt
     6361 cgcgatcgag gtgaaggacg gcggccgcat tcccggcgcc gcgttccgcc aggcgctcgc
     6421 cgacgcggtc acgatgtcgc gcgtgttctc gcgcgtcgcg ccgtcggggc cctacgtgct
     6481 gtcggtggtc gtcaaccggc tcgaggtccc ggagagccgg ttcaacatca acctcgcgcc
     6541 gacggtgcgc atggaggcgg cctggacgct caggcgcgcc ggcacgcgcg cggcgctgtg
     6601 gcaggcgacc gtgcgctcgg agtcgacgag ccagccgtcc gacacgctcg acggcaaggc
     6661 gcggctgcgc atggcgatcg agggcgccgc gcgcgagaac atcgcgtcgg gtctcgcgcg
     6721 actcgcgaag gtgagcctcg ccccgaccta gtccgcgcgg atcccggcct ccttcacgac
     6781 cttcttccag agcgcggcgt ccgagcgcag cgtgcgcgcg aactcctcgg gcgttccgcc
     6841 tgcgggctcc gcgccctgcg cgacgagctg ctcgcgcatc gcgggctcgt gcacgatctt
     6901 cgtcgcctcg gccgcgaggc gggtcacgac cgcgctcggc gttcccgccg gcgcgagcag
     6961 accgtaccac ggcgatagct cgtagccggg aacgccggcc tcggcgacgg tcggcacgcc
     7021 gggcagctcg ggcgagcgct ttgtacccgt gacggcgagc gcgcgcagct tgcccgagcg
     7081 gatgtgcggg agcacggtcg gaatgccgag gaaggcgatc tgcacctggc cgccgacgag
     7141 atccgcggtg gcgggccccg tgcctttgta ggggacgtgc gtgagctgca cgccggccat
     7201 caccgcgaac agcgcggcgg cgaggtgcgg cgtgctgccc gagccgcccg aggcgtagtt
     7261 gatcgcgccg ggcttctgct tcgcgagcgc gatcagctcc ttcaccgatt tcgccgggaa
     7321 cgacggatgc gcgacgagcg cgttcggagc gatcgccatg atcgtgaccg gcgcgaagtc
     7381 tcgcatcgtg tcgtagggca ccttgccgag cagcgggttg atgatgagcg cgcccggccc
     7441 caccgacacg agcgtgtagc cgtcgggcgc gctcttcgcg accgagtcgg tgccgatgtt
     7501 gccgccggca ccgggacggt tctcgaccac gatcggctgg ccgagcgtct cgcccaggcg
     7561 cttgccgacc atgcgcacga tgatgtcgtt cgggccgccg gcgggatacg gcacgacgac
     7621 gtgcacgggg cgggaaggcc agccctgtgc aaaggcgccg caggcgacga gcccgagcag
     7681 cgcgctgaca aacacacgaa tttgcgtcat agtcttgccc ctgcaatcaa cccagcgccg
     7741 atcttagccg accggagacc cgagtgaaac tgcgattgac ccttctgact tttgcgttcc
     7801 tgtcctcgtc cgccctcgcg cagtatccga accgccccat ccacatcgtc gcgcccttcc
     7861 ccgcgggcgg cggctacgac ttcctctcgc gcctgctcgc cgccgagatg acgaagacct
     7921 tccgccagcc cgtggtggtc gagaacaagt cgggcgcgaa cgggaacatc ggcagcgact
     7981 tcgtcgcgaa gagcccggcg gacgggtata ccctgctcat gggcggcaac agcccgctcg
     8041 cgctcaacgt cggcctctac ccgaagctgc cgtacgaccc ggcgaaggac ttcgacgcca
     8101 tctcgcgcgt cgcgacgcag ccgaacctgc tcgccgcgaa ccccgggaag ctcccggtga
     8161 agtcggtggc cgagctggtg aagtacgcaa aggccaatcc cggcaaggtg acctacggtt
     8221 cgaacggcaa cggcagcccg cagcacctcg ccgccgagct gctgaagcgc atggccggga
     8281 tcgacctcgt gcacgtgccc tacaagggcg ccgcgcccac cgcgcaggcg ctgctcgcgg
     8341 gcgaggtcgc ggtcgcgttc aacatcatcc tgctgccgct gccgtacgtg cagtcggggc
     8401 gcctcaacgg gctcgcggtc gcgagctcgc atcgctcccc gctcgcgccg agcctgccga
     8461 ccatggccga gctcggctat ccgatcgaca tcgacacctg gtacggcctg gtcgcgcccg
     8521 cgggaacgcc caaggacgcg atcgcgaagc tgaacgccga gacggtgcgc atcgtcggct
     8581 tgcccgatct gaaggagaag acgcagtcgc agggaatcga gctcggcgga tcgacacccg
     8641 agcagttcgc cgcgttcctg cgcgccgaca tcgccaagtg gtcgaaggcg atccgcgaga
     8701 tgaacatcac catcgattga ggagcgccat gatcctcgac gtcttcaacc atttcatgcc
     8761 gaagtcgatc ttcgaccggc tgtcgcagct cgtccccggg cacatcgcgc tgaccgcgtt
     8821 ccccgagctg ccgacgctct gggacgtgaa cgcgcgcctc aggatgatgg acgagttcgg
     8881 cgagctccag cacgtgctct cgctcgccaa cccgccgatc gagatgctcg gcacccccga
     8941 caagacgccc gagctcgcgc gcatcgcgaa cgacgggctc gccgagctgt gcgcgaagca
     9001 cccggaccgc ttcccgacgt tcatcgcctc gatgccgatg aacaacgtcg aggcctgcgt
     9061 ggccgaagcc gaccgtgcga tcgggcagct cggcgcgcgc gggatccagg tgttcaccaa
     9121 cgtcctcggc aagccgctct ccgcgcccga gttcaggccg ctgttccgcg cgatggcgaa
     9181 gcacgacctg ccggtgtgga tccacccgat ccgcgggccg cagttccccg actacgcgac
     9241 cgagaaggcc tccgagaacg agatctggtt caccttcggc tggccgtacg agaccacggc
     9301 gtgcgtcacg cgcctcatct tctccgggat ctacgacgag ctgcccggcc tcaagatcat
     9361 cacgcaccac atgggcggca tggtcccgtt ctacgccggc aagatcgatc tcggattcga
     9421 ccagatcttc cacggcaaga ccgacgacaa cccggtcgcg aggaaggccg gcctgaagaa
     9481 gcggcccggc gactactacc gcatgctcta cgccgacacc tcggtcaacg gctcggcggc
     9541 ggcggcgcgc tgcggccacg ccttcttcgg cacctcgcac tgcgtgttcg cgaccgacgc
     9601 gcccttcgac gccgagcagg gccgcatgct gatccgcgag accatcaagg cggtcgacgc
     9661 gctcgagatc ccgaaggccg agaaggacca gatcttccac ggcaacgcca ggcgcctctt
     9721 gaagttgtca tcctgagcgc agcgaaggat ctgcttttgg cgaaaaccaa ggacattcgg
     9781 gtccgtcttc gcgacggcga aatggccgcc cacgtcgcct atcccgaccg caccccagcc
     9841 ggcgcgatca tcgccatcat ggagatctgg ggcgtgaacg acaccatgcg ccgccacgcg
     9901 caggagttcg ccgaggccgg gttcgtctgc ctcgtgcccg acctcttctg gcgccaggag
     9961 ccgggcgtcg agctctccga caggaacccc gagcacgtga agaaggcgtt cgatctctac
    10021 tacgagttcg actacgacct cggcgtgcgc gacatggagg atacggtcgc cttcctcgag
    10081 aaaatgcccg ggtgcaacgg caaggtcggc tcggtcggct actgcctcgg cggcaagctg
    10141 aactacctga tgtgctgccg cggcacgagg atggagtgcg cggtcgccta ttacggcacc
    10201 tacatcgagc acaacatccg cgaagcgaag aacctgagca agcccttcat gctgcacatg
    10261 gccatgaagg accgctgggt gcaggccgag gtgaacgacc tgatcgagcg gcgcctctcg
    10321 ccgaatccgc tcgtcacgat ccacaagtat cccgaggccg accacgcctt
//
LOCUS       DATDMW010000396         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1237842, whole genome shotgun sequence.
ACCESSION   DATDMW010000396 DATDMW010000000
VERSION     DATDMW010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237842"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="VI319_16490"
     CDS             complement(<1..1665)
                     /locus_tag="VI319_16490"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY6865493.1"
                     /translation="MPRYLKFALIGLAGLVVVVLAVVAIVAATFDPNDYKPLLIKLVQ
                     EKQQRTLDIPGRIRLTFFPKIGADLGKVSLSERGSKDTFASVESARVSVALLPLLAKK
                     VVVDRVAVDGIRANLKKRKDGTTNFDDLAGGGEKTSPKDQQVLVLAVDGVDLTNADIV
                     YEDDKAGARYELAGATLKIGKLGEGVSDDFSFRGKVRSKSPAADADVSLKTKYTMHLQ
                     KKQIQLKDLSAEVTGSVADLKNVAAGVSGGADVLLSPLSASVSALAVSFKAKQASGDL
                     AAKLEVPALKYAGDRVEAPKLSADVTLPGSSGAMHVTAAGSASANLDKQTAAADLSGK
                     LDESNFTARLAVEKFSPMTYGFDVSVDQLDVDRYTGKPGAKPAPKGGGDGGGAAKSPA
                     SAPAPETPIDLSALKDLNAHGSAKIGKLKVAGINASNVQLQLRAAGGKADVSPLAADL
                     YQGKLAGSASVNASPVRLALKQNLTGVSVGPLLKDLMGQDRLEGRGNVSLDVTSQGDT
                     VSAIKKALGGSARLDLRDGSIRGVNIAQAIRGAKAALGRGGGEGAGK"
     gene            1727..>2629
                     /locus_tag="VI319_16495"
     CDS             1727..>2629
                     /locus_tag="VI319_16495"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865494.1"
                     /translation="MLLAELIAASARVAATRSRLAKIDALAAVLRRLPPDEAALGVAY
                     LSGETRQGKSGVGYAALQAALETAPAAVATLEIPAVDRELDEIARLSGAGSAAERRRR
                     LAGLFARATADEQDFLARLLLGELRQGALEGVMVDAIARAAGLPPARVRAAAMRAGGL
                     PAIAQAALAEGDAGLARFALKIFRPVQPMLAQPAEDVADALERLGEAAFEWKLDGVRL
                     QLHKSGDEVRAYTRNLNDVTEAVPEVVAAVRGARARELILDGETIALRADGTPYPFQE
                     TMRRFGRRLEADELRAAMPLSVYCF"
BASE COUNT          333 a          935 c          954 g          407 t
ORIGIN      
        1 cttgcccgcg ccctcgccgc cgccgcggcc gagcgccgcc ttcgcgccgc ggatcgcctg
       61 cgcgatgttg acgccgcgga tcgagccgtc gcgcaggtcc aggcgcgcgc tgccgccgag
      121 cgccttcttg atcgccgaca cggtgtcgcc ctgcgaggtg acgtcgagcg acacgttgcc
      181 gcggccctcg agccggtcct ggcccatcag gtccttgagc agcgggccga cgctgacgcc
      241 ggtcaggttc tgcttcagcg cgagccgcac cggcgacgcg ttcaccgacg ccgagcccgc
      301 gagcttgccc tggtacaggt cggcggcgag cgggctcacg tcggccttgc cgcccgccgc
      361 gcgcaactgc agctgcacgt tggacgcgtt gatccccgcg accttgagct tgccgatctt
      421 cgcgctgcca tgcgcgttca ggtccttgag cgcggacagg tcgatcggcg tctcgggcgc
      481 gggcgcggac gccggcgatt tcgccgcgcc gccgccgtcc ccgccgcctt tcggcgcggg
      541 cttcgcgccc ggcttgccgg tgtagcgatc gacgtcgagc tggtcgaccg agacgtcgaa
      601 accgtaggtc atgggcgaga acttctccac cgcgagcctg gcggtgaagt tgctctcgtc
      661 gagcttgccc gagaggtcgg ccgcggcggt ctgcttgtcc aggttcgcgc tcgcgctgcc
      721 cgcggcggtg acgtgcatcg cgcccgagga gcccgggagc gtgacgtcgg ccgagagctt
      781 cggcgcctcg acccggtcgc ccgcgtactt gagcgcgggg acctcgagct tcgccgcgag
      841 atcgcccgac gcctgcttcg ccttgaagga cacggcgagc gcggaaaccg aagccgagag
      901 cggcgagagc agcacgtccg cgcctcccga gacgccggcc gcgacgtttt tcaggtccgc
      961 gacgctgccc gtgacttcgg ccgagaggtc cttgagctgg atctgcttct tctggagatg
     1021 catcgtgtac ttcgtcttca gcgagacgtc ggcgtctgcg gccgggctct tcgatcgcac
     1081 cttgccgcgg aacgagaagt cgtcgctcac gccctcgccg agcttgccga tcttcagcgt
     1141 cgcgccggcg agctcgtagc gcgcgccggc cttgtcgtcc tcgtagacga tatcggcgtt
     1201 ggtgaggtcg acgccgtcga cggcgagcac gagcacctgc tggtccttgg gcgaggtctt
     1261 ctccccgccg ccggccagat cgtcgaaatt cgtggtgccg tccttgcgct ttttcaggtt
     1321 ggcccggatg ccgtcgacgg cgacgcgatc gacgacgact ttcttcgcga gcagcggcag
     1381 cagcgcgacc gagacgcgcg ccgactccac cgaggcgaac gtgtccttgc tcccgcgctc
     1441 cgacaggctc accttgccga ggtcggcgcc gatcttgggg aagaacgtga gccggatgcg
     1501 cccgggaatg tcgagcgtcc gctgctgctt ctcctggacc agcttgatca ggagcggctt
     1561 gtagtcgttg gggtcgaagg tggcggcgac gatcgcgacc acggcgagga cgacgacgac
     1621 caggccggcc agtccgatca gcgcaaactt gaggtaacgc ggcatgatgg ctcgttggac
     1681 aatgcggggt cgagggaatt cgtgcggatg agctcatgat aacgccatgc tgctcgccga
     1741 gctgatcgcc gcctccgcgc gcgtggcggc gacgcgcagc cgcctggcga agatcgacgc
     1801 gctcgccgcc gtgctgcggc gcctgccgcc cgacgaggcc gcgctcgggg tcgcgtacct
     1861 ctcgggcgag acgcgccagg ggaaatcggg cgtcggctac gcggcgctgc aggcggcgct
     1921 cgagaccgcg cccgcggccg ttgcgacgct cgagatcccg gccgtcgacc gcgagctcga
     1981 tgaaattgca cgattgagcg gcgccgggtc cgccgccgag cggcggcggc ggctggccgg
     2041 tctgttcgcg cgcgccaccg ccgacgagca ggacttcctc gcgcggctgc tgctcggcga
     2101 gctgcgccag ggcgcgctcg aaggcgtcat ggtcgacgcc atcgcgcgcg cggccggcct
     2161 gccgccggcg cgcgtgcgcg cggccgcgat gcgcgcgggc gggctacccg cgatcgcgca
     2221 ggccgcgctc gccgagggcg acgccggtct cgcgcgcttc gcgctgaaga tcttccggcc
     2281 ggtgcagccg atgctcgcgc agcccgccga ggacgtggcc gacgcgctcg agcgcctcgg
     2341 cgaggccgcg ttcgagtgga agctcgacgg cgtgcgcctg cagctgcaca agtcgggcga
     2401 cgaggtgcgc gcgtacacgc gcaacctgaa cgacgtcacc gaggcggtgc ccgaggtggt
     2461 cgccgccgtg cgcggcgccc gggcgcgcga gctgatcctc gacggcgaga cgatcgcgtt
     2521 gcgcgccgac ggcacgccct acccgttcca ggagacgatg cgccgcttcg gtcggcgcct
     2581 ggaggccgac gagctgcgcg ccgcgatgcc gctctcggtg tattgcttc
//
LOCUS       DATDMW010000397         7170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1612415, whole genome shotgun sequence.
ACCESSION   DATDMW010000397 DATDMW010000000
VERSION     DATDMW010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612415"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..822
                     /locus_tag="VI319_16500"
     CDS             43..822
                     /locus_tag="VI319_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY6865495.1"
                     /translation="MSDLITRREGRVGWIVFSNLTKRNALSYDIWRGIPDAVRALALD
                     TGVRVIALRGDGADFSAGADVAEFEHARDSISATSSYNVVVDEANEALVSVPKPTLAM
                     IRGLCYGGGLAMALHCDMRICADNAQFSLQAAKLGFGISYASTMRFAHVVGPAHSAEV
                     MFSGKIFGAEEAYHMHLVNRVAPADQLDAVFGEWSEMFANAAPLAVASIKRSLVEGYK
                     DPDRRDLRSVAAMIEACYTSDDYREGRAAFRAGRKPEFKGR"
     gene            complement(833..2116)
                     /locus_tag="VI319_16505"
     CDS             complement(833..2116)
                     /locus_tag="VI319_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY6865496.1"
                     /translation="MEILVLFGCVLVLVLLNIPIAVALGIVAIAAIVVTQGVASLPNL
                     PVVLYNGATNFPLIAIPLFILAGSIMNASGISRRLIAFASAVLGWIRGGLSIVSIGTS
                     MFFAEISGSAVADVAALGSVLIPAMERRGYSKEFACAVMSSSATLAVIIPPSIPMILY
                     AVIAGTSVVQLFVAGIVPGIIGGLGLMGMAYWFARRYNFPVEESFRLAHVWKTFKEAI
                     WAFLMPVIILGTIFGGVVTATEGAAMAVIAALFIGLFVYRELDLAHLKQAVIEGGVQT
                     AVVMLLVATSALLGVYLTEAQVPQKLAASVASFTTNKWVVLAILNVLFLILGLFLHSA
                     AAIILTVPVVMPLVNAAGIDPVHFGLIVTINLGIGQQTPPVASVLMTACAIGKADMWE
                     VTKINVWFVAVLMAVLLLVTYVPATGMWLVDLLYR"
     gene            complement(2120..2638)
                     /locus_tag="VI319_16510"
     CDS             complement(2120..2638)
                     /locus_tag="VI319_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY6865497.1"
                     /translation="MRIEAVRSAFERFLEVVVIVLMVTLSAEVTLGIVYRTIGSSLVW
                     YDEVAAILLAWLTFYGSALAALKRAHIGFPGLVNSLAPRWRVPAVVFAEACVIAFFAL
                     LGWVGYAVLDVLSTDFLVSLPSVSVAWTQSVIPIGALLFILAEVLTVPGLIASARGRG
                     AIASSDLAEKLH"
     gene            complement(2662..3636)
                     /locus_tag="VI319_16515"
     CDS             complement(2662..3636)
                     /locus_tag="VI319_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY6865498.1"
                     /translation="MRHVRFALGALAALGLSGPVHAQTELKLGHVGEPGSLIGAAADE
                     FARRANAKLGGKFKVVTYGSSQLGGDKEMMQKMKLGTIDMVEPSTIMSSEADIFGVFE
                     MPYLVKSREHMKRIEKELFWSKIEPDAEKKGVKVLAVWENGFRHITNNKHPINTPADL
                     KGIKLRVPEGKWRVKMFQAYGANPSPMKFSEVFTALQTGVMDGQENPFSQIASAKFYE
                     VQKYLSLTGHVYTPSYLVVGTRKWNTYPADVRKILEETAKDTQAWVYEKAAKDDEQLL
                     AMMKKAGIQVNNANKDAFIAASKPVYDEFAKEVPGAKQVIDRAIELGK"
     gene            complement(3702..4901)
                     /locus_tag="VI319_16520"
     CDS             complement(3702..4901)
                     /locus_tag="VI319_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6865499.1"
                     /translation="MAHRSGRHFLQIPGPTNVPDRVLRAIDNATMDHRGPEFGRMGLE
                     ILEGLKTVFQTKQPVVIYPASGTGAWEAALVNTLSPGDRVLMCETGHFASLWQKMAGK
                     LGLSVELIPTDWRRGADPAAIEAKLKEDRGHTIKAVCVVHNETSTGVTSRIGPVRKAI
                     DAAGHPALFMVDTISSLASIDYRHDEWGVDVTVAGSQKGLMLPPGLSFNCVSERARKA
                     SQSAKLPRSYWSWDEMLSNNKSGYFPYTPATNLLYGLREALRMLEEEGLQNVFARHNR
                     HAEATRRAVRAWGLEILCAVPEEYSSSLTAVTMPAGHDADRLRKIILDAYDMSLGTGL
                     GRLAGKVFRIGHLGDFNDLALAGTLAGVEMGLELAAVPHRKGGVLAALDHLAECAKGA
                     SAARKAA"
     gene            4971..5675
                     /locus_tag="VI319_16525"
     CDS             4971..5675
                     /locus_tag="VI319_16525"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEY6865500.1"
                     /translation="MESTRAFLGSHLTDFAMPLTIDVVSDVVCPWCYVGKRRLERALE
                     LYRAKHPGAPAPEVTYHPFQLNPDVPREGVPRAEYIERKFGPRGYGAHERLVHAGKPL
                     GIDFAFDRIERQPNTLPAHALIEWARRRGAQPAVKEALMKAFFVDGRDLTDPQTLVAV
                     AAESGLDRNEAETAIADAELLKNVAEEEEHAKAMGIHGVPFFIFNRRLAVEGAQPSDV
                     LLEAMLEAEKQPAEAK"
     gene            5683..>7170
                     /locus_tag="VI319_16530"
     CDS             5683..>7170
                     /locus_tag="VI319_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6865501.1"
                     /translation="MDRAPGPVFTVSFHPRIGDSALAARLRREIEGEVLFDAGSRGRY
                     STDASIYQIEPVGVVVPKSEADAKIAMQIAVEEGVPILARGAGSSQCGQTVGAALVID
                     NSKHLNQVVAFDRERATAVVQPGLVLDALNAQLRPHGLWFPVDVSTSAQATLGGMAGN
                     NSCGSRSIRYGNMVHNVAGIDAVLADGSEHHFGVVASDLSRLAGDARYAELARKVRSI
                     AEREYLEIESRWPKVLRRVQGYNLDLVRPASDWNFAQLLVGSEGTLAYSKRIHLKLSP
                     IPKQRALGVCHFATFRKSMEAPQHIVKLGPSAVELVDRTMIGLARANEAFRPIVEKFI
                     SGDPEAILLVEFSGDDRDTQVKKLKELTALMGDLGHPGSVIEIVDAQLQRDVWEVRKA
                     GLNIMMSMKGDGKPVSFIEDCAVPLENLADYTERLTEVFRKHGTEGTWYAHASVGTLH
                     VRPVLTMKEDGAKKMRAIAEEACALVKSYKGAAYSGEHGDGLVRSE"
BASE COUNT         1197 a         2420 c         2449 g         1104 t
ORIGIN      
        1 gtcgcaggaa catcgtttga cgagcccacg ggaggggtgg ccatgtcgga tctgatcacg
       61 cggcgggaag gccgggtcgg gtggatcgtg ttctcgaacc tgaccaagcg caacgcgctc
      121 tcgtacgaca tctggcgcgg catccccgac gcggtgcgcg cgctcgcgct cgacacgggt
      181 gtgcgggtga tcgcgctacg cggggacggc gccgacttct cggcgggcgc cgacgtcgcc
      241 gagttcgagc acgcgcgcga ctcgatcagc gcgacctcga gctacaacgt cgtggtcgac
      301 gaggcgaacg aggcgctggt cagcgtcccg aagccgacct tggccatgat ccgcggcctg
      361 tgctacgggg gcgggctggc gatggcgctc cactgcgaca tgcgcatctg cgcggacaac
      421 gcgcaattct cgctgcaggc ggcgaagctc ggcttcggca tctcgtacgc gagcacgatg
      481 cgcttcgcgc acgtcgtcgg acccgcgcac agcgccgagg tcatgttctc ggggaagatc
      541 ttcggcgccg aggaggccta tcacatgcat ctcgtcaacc gcgtggcgcc cgccgaccag
      601 ctcgacgccg tgttcggcga gtggtccgag atgttcgcga acgcggcgcc gctcgcggtc
      661 gcctcgatca agcgctcgct cgtcgagggc tacaaggacc cggaccggcg cgacctgcgc
      721 tcggtggcgg cgatgatcga agcctgctac acgagcgacg actaccgcga ggggcgcgcg
      781 gcgttcaggg cggggcgcaa gcccgagttc aaggggcgtt gagccgcgcg cttcagcggt
      841 agagcaggtc gaccagccac atcccggtcg ccggcacgta ggtgaccagg agcagcaccg
      901 ccatcagcac cgcgacgaac cacacgttga tcttcgtgac ctcccacatg tcggccttgc
      961 cgatcgcgca ggcggtcatg agcacgctcg caaccggggg cgtctgctgg ccgatgccga
     1021 gattgatggt cacgatcagg ccgaaatgca ccgggtcgat cccggccgcg ttgacgagcg
     1081 gcatgacgac cggcacggtg aggatgatcg ccgccgcgga atgcaggaac aggcccagga
     1141 tcaggaaaag cacgttcagg atggcgagca cgacccactt gttcgtcgtg aaagaggcga
     1201 cgctggccgc gagcttctgc gggacttgcg cctcggtgag atagaccccg agcagggcgc
     1261 tggtcgcgac gagcagcatg acgaccgcgg tctgcacgcc gccctcgatg accgcctgct
     1321 tcaggtgcgc gaggtcgagc tcgcggtaga cgaacaaccc gatgaacagg gccgcgatca
     1381 ccgccatcgc ggccccttcg gtcgccgtca cgacgcctcc gaagatggtc ccgaggatga
     1441 tgaccggcat gaggaaggcc cagatcgcct ccttgaaggt cttccacacg tgcgcgaggc
     1501 ggaacgattc ctcgaccggg aagttgtagc ggcgcgcgaa ccagtaggcc atgcccatca
     1561 ggcccagtcc gccgatgatg ccgggcacga tgccggcgac gaacagctgc accaccgacg
     1621 tcccagcgat cacggcgtac aggatcatcg ggatcgacgg cgggatgatg accgcgagcg
     1681 tcgcggacga cgacatcacg gcgcacgcga actccttcga gtagccgcgc cgctccatcg
     1741 ccgggatcag caccgagccg agcgccgcga cgtccgcgac cgccgagccc gagatctccg
     1801 cgaagaacat cgacgtcccg atcgacacga tcgacaggcc gccgcggatc cagccaagga
     1861 ccgccgaggc gaatgcgatc aggcgccgcg agatgcccga cgcgttcatg atcgagccgg
     1921 cgaggatgaa cagcgggatc gcgatcagcg ggaagttggt cgcgccgttg tagagcacga
     1981 ccgggaggtt gggcaggctc gcgacgccct gcgtcacgac gatggctgcg atggccacga
     2041 tgccgagcgc gaccgcgatc gggatgttga gcaggacgag gaccagcacg cagccgaaga
     2101 ggacgaggat ttccatgttt cagtgcagct tctcggcgag atcgctcgac gcgatcgcgc
     2161 cgcggccgcg cgcggacgcg atcagccccg ggaccgtcag gacctcggcg aggatgaaga
     2221 gcagggcgcc gatcgggatc accgactggg tccacgcgac cgagaccgag ggcaggctca
     2281 ccaggaagtc ggtcgagagc acgtcgagca ccgcgtaccc gacccagccg agcagtgcga
     2341 agaacgcgat cacgcatgcc tccgcgaaga cgaccgcggg cacgcgccag cgcggcgcca
     2401 gcgagttcac cagcccgggg aagccgatgt gggcgcgctt gagcgccgcc agggccgagc
     2461 cgtagaacgt gagccacgcg agcaggatgg ccgcgacctc gtcgtaccag acgagcgagc
     2521 tgccgatcgt gcggtacacg atcccgagcg tcacctcggc cgagagggtg accatcagga
     2581 cgatgaccac gacttccagg aagcgctcga acgccgagcg cacagcttcg atgcgcatgc
     2641 tcctccccga aaacggcgcg gctacttccc gagctcgatg gcgcggtcga tgacctgctt
     2701 cgcgccggga acttccttcg cgaactcgtc gtacaccggc ttcgacgccg cgatgaacgc
     2761 gtccttgttg gcgttgttca cctggatgcc ggcttttttc atcatcgcga gcagctgctc
     2821 gtcgtccttg gcggccttct cgtacaccca ggcctgcgtg tccttcgccg tctcctcgag
     2881 gatcttgcga acgtccgcgg ggtacgtgtt ccacttgcgc gtgcccacca ccaggtacga
     2941 gggcgtatag acgtggcccg tgagcgagag gtacttctgc acctcgtaga acttcgcgct
     3001 ggcgatctgc gagaacggat tctcctggcc gtccatgacg ccggtctgca gcgcggtaaa
     3061 cacctccgag aacttcatcg ggctcggatt ggcaccgtac gcctggaaca tcttcacgcg
     3121 ccacttgccc tcggggacgc gcagcttgat gcccttcagg tccgccggcg tgttgatcgg
     3181 atgcttgttg ttggtgatgt ggcgaaagcc gttctcccac acggcgagga ccttgacgcc
     3241 ctttttctcc gcgtccggct cgatcttcga ccagaagagc tccttctcga tgcgcttcat
     3301 gtgctcgcgg ctcttcacga ggtacggcat ctcgaacacg ccgaagatgt cggcctcgga
     3361 ggacatgatc gtcgacggct cgaccatgtc gatggtcccg agcttcatct tctgcatcat
     3421 ctccttgtcg ccgccgagct ggctcgagcc gtaggtcacc accttgaact tgccgccgag
     3481 cttggcgttg gcgcgccggg cgaactcgtc ggctgccgcg ccgatcagcg agccgggctc
     3541 gccgacgtgg ccgagcttga gctcggtctg cgcgtggacg ggtcccgaaa gaccgagcgc
     3601 cgcaagcgct cccagggcga atcggacgtg tctcatggct tctcctcggg tcaaaaaaaa
     3661 gcgccgcggt gtcgtcgtgt tccgcccgcg gctgcgaatc gtcatgcggc cttgcgcgcg
     3721 gccgaagcgc ctttcgcgca ctcggccagg tgatcgagcg ccgcgagcac gccgcccttc
     3781 ctgtgcggca ccgccgccag ctccaggccc atctcgacgc cggcgagcgt gcccgcgagc
     3841 gcgagatcgt tgaagtcgcc gagatggccg atgcggaaca ccttgccggc gagacggccg
     3901 agccccgtgc cgagcgacat gtcgtaggcg tcgaggatga tcttgcgcag gcggtcggcg
     3961 tcgtggcccg cgggcatcgt caccgcggtg agcgagctcg agtactcctc gggaaccgcg
     4021 cacaggatct cgaggcccca ggcgcgcacg gcgcgccggg tggcctcggc gtggcggttg
     4081 tggcgcgcga acacgttctg cagcccttcc tcctccagca tgcgcagcgc ctcgcgcagc
     4141 ccgtagagca ggttggtcgc gggcgtgtac gggaagtagc cgctcttgtt gttcgagagc
     4201 atctcgtccc agctccagta cgagcgcggc agcttcgccg attgcgacgc cttgcgcgcc
     4261 ctctcgctca cgcagttgaa cgagaggccg ggcggcagca tcagcccttt ctgcgatccg
     4321 gccacggtga cgtcgacgcc ccattcgtcg tggcggtagt cgatcgacgc gagcgaggag
     4381 atggtgtcga ccatgaaaag cgcgggatgg ccggcggcgt cgatggcctt gcgcacgggg
     4441 ccgatgcggc tggtcacccc ggtcgaggtc tcgttgtgga cgacgcatac cgccttgatg
     4501 gtgtgcccgc ggtcttcttt cagcttcgcc tcgatcgccg ccggatccgc gccgcggcgc
     4561 cagtcggtcg ggatgagctc gaccgagagc ccgagcttgc ccgccatctt ctgccacagc
     4621 gacgcgaagt ggccggtctc gcacatcagc acgcggtcgc ccggcgacag cgtgttgacg
     4681 agcgccgcct cccaggcgcc ggtccccgac gcggggtaga tcaccaccgg ctgcttcgtc
     4741 tggaacaccg tcttcaggcc ctccagaatc tcgagtccca tgcgcccgaa ctccggtccg
     4801 cggtgatcca tcgtcgcgtt gtcgatcgcg cgcagcacgc ggtcgggcac gttggtcggc
     4861 ccggggatct gcaggaagtg tcggccgcta cggtgcgcca tggcgggggc tcgaatcggg
     4921 attggagtcg gctaacttaa tcgaaaagcg ctggtgccac aactggtatc atggaatcaa
     4981 cccgcgcgtt cctcgggtcc catctcacgg atttcgccat gcccctcacc atcgatgtcg
     5041 tctccgacgt ggtctgcccg tggtgctacg tcggcaagcg gcgcctcgag cgcgcgctcg
     5101 agctctaccg cgcgaagcac cccggcgcgc ccgcgcccga ggtcacctac cacccgttcc
     5161 agctgaaccc cgacgtgccg cgcgaaggcg tgccgcgcgc cgagtacatc gaacgcaagt
     5221 tcggcccgcg cggctacggc gcgcacgagc gcctcgtgca cgcgggcaag ccgctcggca
     5281 tcgacttcgc gttcgaccgc atcgagcgcc agccgaacac gctgcccgcg cacgcgctga
     5341 tcgagtgggc gcgccgacgc ggcgcgcagc ccgcggtgaa ggaggcgctg atgaaggcgt
     5401 tcttcgtcga cggccgcgat ctcaccgatc cgcagacgct ggtcgcggtc gccgccgagt
     5461 cgggcctcga ccgcaacgag gccgagaccg cgattgccga cgccgagctg ctgaagaacg
     5521 tcgccgagga ggaggagcac gcgaaggcca tgggcattca tggcgtgcct ttcttcatct
     5581 tcaaccggcg cctcgcggtc gagggcgcgc agccgtccga cgtgctgctc gaggcgatgc
     5641 tcgaggccga gaagcagccc gccgaagcga agtaggcgat ccgtggaccg cgcgccgggc
     5701 ccggtcttca ccgtctcgtt ccacccgagg atcggggact cggcgctcgc ggctcgcctg
     5761 cgccgcgaga tcgagggcga ggtgctcttc gacgcgggca gccgcggacg ctattccacc
     5821 gacgcctcga tctaccagat cgagccggtc ggagtcgtcg tgccgaagtc cgaggccgac
     5881 gcgaagatcg cgatgcagat cgcggtggag gagggcgttc ccatactcgc gcggggagcg
     5941 ggcagctcgc agtgcgggca gacggtcggc gcggcgctcg tcatcgacaa cagcaagcac
     6001 ctgaaccagg tcgtcgcctt cgacagggaa cgcgcgacgg cggttgtgca gccgggcctg
     6061 gtgctcgacg cgctgaacgc gcagctgcgc ccgcatggcc tctggtttcc cgtcgacgtg
     6121 tcgaccagcg cgcaggcgac gctcggtggc atggcgggca acaactcgtg cggctcgcgc
     6181 tcgatccgct acggcaacat ggtgcacaac gtcgccggca tcgacgcggt gctcgccgac
     6241 ggcagcgagc atcacttcgg tgtcgtcgct tcggacctct cgcgcctcgc gggcgacgcg
     6301 cgctacgccg agctcgcgcg gaaggtgcgc tcgatcgccg agcgcgagta cctcgagatc
     6361 gaatcgcgct ggcccaaggt gctgcgccgc gtgcagggct acaacctcga cctggtgcgg
     6421 cccgcctccg actggaactt cgcgcagctg ctggtgggct ccgaagggac gctcgcgtac
     6481 tcgaagcgca tccacctgaa gctgtcgccg atcccgaagc agcgcgcgct cggcgtgtgc
     6541 cacttcgcga ccttccgcaa gtcgatggaa gcgccgcagc acatcgtcaa gctcggcccg
     6601 agcgcggtcg agctggtcga ccgcaccatg atcgggctcg cgcgcgcgaa cgaggccttc
     6661 cggccgatcg tcgagaagtt catctcgggc gatccggaag cgatcctgct ggtcgagttc
     6721 tcgggcgacg accgcgatac gcaggtgaag aagctgaagg agctgaccgc cctcatgggc
     6781 gacctcggcc atcccgggag cgtgatcgag atcgtcgacg cccagctgca gcgcgacgtc
     6841 tgggaggtac gcaaggcagg gctgaacatc atgatgtcga tgaaggggga cggcaagccc
     6901 gtgtccttca tcgaggactg cgccgtgccg ctcgagaatc tcgccgacta caccgagcgc
     6961 ctgaccgagg tcttccgcaa gcacggcacc gagggcacgt ggtacgcgca cgcgtcggtc
     7021 ggcacgctgc acgtgcgccc ggtgctcacc atgaaggagg acggcgcgaa gaagatgcgc
     7081 gcgatcgccg aggaggcgtg cgcgctcgtg aagagctaca agggcgccgc gtactcaggc
     7141 gagcacggcg acggcctggt gcgctcggag
//
LOCUS       DATDMW010000398        10193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_989038, whole genome shotgun sequence.
ACCESSION   DATDMW010000398 DATDMW010000000
VERSION     DATDMW010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989038"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VI319_16535"
     CDS             <1..630
                     /locus_tag="VI319_16535"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY6865502.1"
                     /translation="ALTARLPAGPDDGPRLELAQPPAHGQLERIPRPSAQSHVLAGMP
                     GLARDDPDYFSLFVGNFVLGGGGFVSRLYNEVREKRGYAYSAYSYFLPLAARGPFLMG
                     LQTRNDQADEALKQARAVLDDFIKNGPTAKELAAAQRSLVGSFPLRLDSNRKILDQVA
                     MIGFYRLPLDWLDKFIPGIKAVTLESVRSAFSRRVRPEELTTVVAGAAQ"
     gene            617..1198
                     /gene="rsmD"
                     /locus_tag="VI319_16540"
     CDS             617..1198
                     /gene="rsmD"
                     /locus_tag="VI319_16540"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010113859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY6865503.1"
                     /translation="MRRSRPNTVRIIGGRWRSRIVDFGDVAGVRPTPDRVRETLFNWL
                     GQTLADLDCLDLFAGSGALGFEAASRGARSVVMVEKHPRVLESLRASARALDAANVRL
                     VHGDALEFLRVPGVRYDVVFVDPPYRLALAEQALSLLPPVLAPDARVYVESDAPLRVA
                     AGWTMIRQARAGQVHYHLLGRDPDDQGRLPRNV"
     gene            1167..1655
                     /gene="coaD"
                     /locus_tag="VI319_16545"
     CDS             1167..1655
                     /gene="coaD"
                     /locus_tag="VI319_16545"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY6865504.1"
                     /translation="MIRAVYPGTFDPITRGHEDLVRRASALFDELVVGVAGSSPKQPF
                     FNLAERVRLAREVLAPYENVTVESFDGLLMDFVRAQKARAILRGLRAVSDFEYEFQMA
                     GMNRNLYPDVETLFLTPSEQYMFISATIVREIASLGGDVGQFVHPRVRQALAKKVDER
                     KK"
     gene            complement(1665..2477)
                     /gene="mutM"
                     /locus_tag="VI319_16550"
     CDS             complement(1665..2477)
                     /gene="mutM"
                     /locus_tag="VI319_16550"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY6865505.1"
                     /translation="MPELPEVEITRRGIEPFVAGRTITGVAIRNPNLRWRVPRTLART
                     IIGQQVKRVDRRGKYLLLECGSGSLILHLGMSGSLRVIDPRAPASAHDHVDLVFGDKA
                     LRLRDPRRFGALLWQPGDVRLHRLLSGLGVEPLSEEFSADYLYAATRRRQAGIKQVLM
                     NANIVVGVGNIYANESLFRAGVHPRRRARRVTRAECERLVISIRETLRDALAAGGSSL
                     RDFVHSDGASGYFQQSYYVYDRAGLPCRVCASAIRVARLGSRSSFFCTACQK"
     gene            2597..4357
                     /locus_tag="VI319_16555"
     CDS             2597..4357
                     /locus_tag="VI319_16555"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6865506.1"
                     /translation="MRIPVRGIAVALLAGALAACAGRAVKPGSDDDGGDADDGDASSF
                     FETPERGVAPALPPQDLTETVLYEFLLAEIATQRGDVTLAAQAYVDLAKRTRDPRIAR
                     RATEVAMYARMANAAVEAAQIWQETEPQSTRARQALAGLLVNAGRYDEALPHFDKLLA
                     DGAVKPAETFSQLGRALASAQDKKSALKLVRTLAQRYPRVPQAHFAVAQTAAGAGDEE
                     LALAEIRQAEQLRPDWDNAVLLEAQLLQRKSPDAAAARLETFLKSNPGSREVRLDYAR
                     LLVSQKKIAEARGEFQKLLAAYPDNSDVTFAVALLSLQLNDYDAAEANLKRLLQGSYR
                     DKNGVRLYLGQIAEDRKKLPEALRWYDEITGGEHYLAARIRSAQVLSKQGKLAAARER
                     LHQTEPQNGQQRIQLILAEAQLLRDANQPKAAFDMVQQALDKLPNQPDLLYDHAMLAE
                     KLDRMDILESSLRKLIALRPDYAHAYNALGYSLADRNERLPEARELIEKALKLSPDDS
                     FIIDSMGWVLYRQGKTKDALEWLQKAYAARPDAEIAAHLGEVLWVLGRRDDAERVLRD
                     AAQKDPGNEAIASTLKRLKR"
     gene            4369..4902
                     /gene="lolB"
                     /locus_tag="VI319_16560"
     CDS             4369..4902
                     /gene="lolB"
                     /locus_tag="VI319_16560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEY6865507.1"
                     /translation="MRRLALVWVAAVAIAACATLPKPETPQGEGAFELHGRVAVRYGS
                     DGASGNIAWRHSPQADDLLITSFLGQGVARIRRSDGEVQLVADGKEHRAPDAETLTER
                     VLGWRLPLEGLPDWVQGHPAPGRAEQLRREGERLVAFVQDDWKVEYQAFDGDRPSRLR
                     LTRPNLEIRLIVDQWSG"
     gene            4899..5747
                     /gene="ispE"
                     /locus_tag="VI319_16565"
     CDS             4899..5747
                     /gene="ispE"
                     /locus_tag="VI319_16565"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY6865508.1"
                     /translation="MSLWDRAWPAPAKLNLFLHVVGRREDGYHLLQSAFRLIDRRDRL
                     RYAPRDDGRVALMRPLPGIDESTELSVRAARLLLAESGSRQGVDIEIEKSIPVGGGLG
                     GGSSDAATTLVVLNRLLRLGASPEDLARLGLSLGADVPFFVFGRNAFAEGVGERLTAL
                     ALPAAWYAVLVPPLAVSTKAIFDAPELTRNSKTIKISSFSAGFGRNDLEPVVCRRHEE
                     VAAHLAWLKQFGDARLSGSGACVFAEFPSEREARDVLSGMPDKMTGFVARGLDRHPLA
                     DLLDHG"
     gene            5752..5828
                     /locus_tag="VI319_16570"
     tRNA            5752..5828
                     /locus_tag="VI319_16570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5786..5788,aa:Gln,seq:ttg)
     gene            5937..6887
                     /locus_tag="VI319_16575"
     CDS             5937..6887
                     /locus_tag="VI319_16575"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY6865509.1"
                     /translation="MAYESLMVFTGSANPLLAHAVVRRRNIPLGHATVGTFSDGEIMV
                     EVLENVRGKDCFVLHSTCAPANESLMEVLLMVDALKRASAARVTAAIPYFGYARQDRR
                     PRSARVAISAKVVANMLQAAGVDRVLTMDLHADQIQGFFDVPVDNIYATPVLLGDVWK
                     HQYENLLVVSPDVGGVVRARAMAKRLESDLAIIDKRRPQPNVSEVMNVIGDVKGRTCV
                     IMDDIVDTANTLCKAAEALKNEGATRVVAYCTHPVLSGGAVERIAASSMDELVVADTI
                     PLSKAGQACKKIRVLSVAGLLAETILRISNEDSVSSLFME"
     gene            6948..7682
                     /locus_tag="VI319_16580"
     CDS             6948..7682
                     /locus_tag="VI319_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY6865510.1"
                     /translation="MEVQAQARKLQGTGASRRLRREGKVPGIVYGGSQPALTIELDHN
                     ALFHHLRNEKFHASILTLKVDGATEQVLLRAVNMHPFKLQVQHIDFQRVSAKEKIHMK
                     VPLHFVNAEASPAVKVSGGIISHVANELDVRCLPADLPEFIEVDLSKLEINDSIHVRD
                     LALPKGVELMLRGQENPPVATAQVPKAAIEEEEAAAAAAAATAEGTAAPEATPSAADV
                     PAVKQKAPEAAAPAAEDKGKGKSEKK"
     gene            7734..8315
                     /gene="pth"
                     /locus_tag="VI319_16585"
     CDS             7734..8315
                     /gene="pth"
                     /locus_tag="VI319_16585"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY6865511.1"
                     /translation="MASIRLIAGLGNPGREHDRDRHNVGFMFASRLAAASRVDMRSSS
                     RYAGLLGKIAHPAGDIWLLMPQTYMNRSGESVGPLVHFYKIRPQEVLVVHDELDFPPG
                     VVKIKLGGGVAGHNGLKDIAARLGSHDFWRLRIGIGHPGDKAVVPDYVLKSPSPEDRE
                     QIERALERSLEVAPLMLDGKMEAAMLKLHTKAA"
     gene            8317..9411
                     /gene="ychF"
                     /locus_tag="VI319_16590"
     CDS             8317..9411
                     /gene="ychF"
                     /locus_tag="VI319_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY6865512.1"
                     /translation="MSVQCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     EVPDPRLARLADIAQPQKIVPAVVEFVDIAGLVAGASKGEGLGNQFLANIRECDAIAH
                     VVRCFEDANVVHVAGKVDPVSDIETINTELALADLAAVEKQLAKNQKLARAGGDKEAQ
                     RLVAALEKVQAALDQGKPARGIPLSKEELAVIRPLFLLTMKPTMYVANVSEHGFAGNP
                     LLEKVQVYAKAEGAPVVAISAALEAQIADLSDEDKKVFLEDMGLEEPGLDRLIRAGYA
                     LLGLQTYFTAGPKEVRAWTIPAGATAPQAAGVIHTDFERGFIRAEVAAYDDYVGCKGE
                     QGAREAGKLRLEGKDYVVRDGDVVHFRFNV"
     gene            complement(9426..>10193)
                     /locus_tag="VI319_16595"
     CDS             complement(9426..>10193)
                     /locus_tag="VI319_16595"
                     /inference="COORDINATES: protein motif:HMM:NF025089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6865513.1"
                     /translation="LRGAAPTISVIVCSITPSKYERVTANYAALLAGVDHEIVGVHDA
                     RSLAEGYNRGVRRARGEILVFSHDDIEIVAPDFAAKLANRLSDFALVGVAGTDRVCGG
                     SWIDAGWPYVHGQIGMPERGGRGIVTTAYLLSERSAGAMQALDGVFFACRRAVAEKIG
                     FDERTFDDWHLYDLDFSYSAAVAGFSVGVAGDILLVHQSAGKFSCTWLDHARRFVAKH
                     KIADPRTYTFKGMALPSAVLRSAQEWRAWTHYMIDGR"
BASE COUNT         1681 a         3427 c         3529 g         1556 t
ORIGIN      
        1 gcgctcacgg cgcggctgcc cgcgggcccc gacgacgggc cgcgcctcga actggcgcag
       61 ccgcccgcac acggacagct cgagcgaatt ccccgcccct cggcgcaaag tcatgtgctc
      121 gccggcatgc caggcctcgc gcgcgacgac ccggattact tctccttgtt cgtgggcaat
      181 ttcgtgctcg gcggcggcgg cttcgtctcg cgcctctaca acgaggtgcg cgagaagcgc
      241 ggttacgcct acagcgccta cagctatttc ctgccgctcg cggcgcgcgg gcctttcctc
      301 atgggcctgc agacgcgcaa cgaccaggcc gacgaggcgc tcaagcaggc gcgcgcggtg
      361 ctcgacgact tcatcaagaa cggtccgacg gcgaaggagc tcgccgccgc gcagcgcagc
      421 ctggtcggca gctttccgct gcgactcgat tcgaaccgca agattctgga tcaggtggcc
      481 atgatcggct tctaccgcct gccgctcgac tggctggaca agttcatccc cggcatcaag
      541 gcggtgacgc tcgagagcgt gcgctcggca ttctcgcgcc gcgtgcgccc ggaggagctg
      601 acgacggtcg tagccggtgc ggcgcagtag gccgaacacc gtacgcatca tcggcggtcg
      661 ctggcgcagc aggatcgtcg atttcggcga tgtcgcgggc gtgcgcccca cgcccgaccg
      721 cgtgcgcgag acgctgttca actggctcgg gcagacgctc gccgacctgg actgcctgga
      781 cctcttcgcc ggcagcggcg cgctcggctt cgaggcggcg tcgcgcggcg cgcgctcggt
      841 ggtgatggtg gagaagcatc cgcgcgtgct cgagtcgctg cgcgcgtccg cgcgcgcgct
      901 cgatgcagcc aatgtgcgcc tcgtgcacgg cgatgcgcta gaattcctgc gcgtccctgg
      961 cgtgcggtac gacgtcgtgt tcgtggatcc gccctaccgg ctggcgctcg cggagcaggc
     1021 cctgtcgtta ttgccgccgg tgctcgcgcc ggacgcgcgc gtgtacgtgg aaagcgatgc
     1081 gccgctccgt gttgccgcgg gctggacgat gatcaggcag gcccgggccg gccaggtgca
     1141 ctaccatctc ttgggaaggg atcccgatga tcagggccgt ctaccccgga acgtttgacc
     1201 cgatcacgcg cgggcacgag gatctcgtgc ggcgcgcctc cgcgctgttc gacgagctgg
     1261 tcgtcggcgt cgcgggcagc agcccgaagc agccgttctt caacctcgcc gagcgcgtgc
     1321 gactcgcgcg cgaggtgctc gctccctacg agaacgtgac ggttgagtcg ttcgacgggc
     1381 tgctcatgga cttcgtgcgc gcgcagaaag cgcgcgcgat cctgcgcggg ctgcgcgcgg
     1441 tgtccgactt cgagtacgaa ttccagatgg ccgggatgaa ccgtaacctg tatcccgacg
     1501 tcgagacgct gttcctgacg ccttcggagc agtacatgtt catctcggcg accatcgtgc
     1561 gcgagatcgc ctcgctcggc ggcgacgtcg gacagttcgt gcacccgcgc gtcaggcagg
     1621 cgctcgcgaa gaaggtggac gaacgcaaga aatagcctcg ccggctactt ctggcacgcg
     1681 gtgcagaaga agctggagcg gctgcccagg cgcgcgaccc gtatcgcgga ggcgcacacg
     1741 cggcagggca accccgcgcg gtcgtagacg taatagctct gctggaaata tcccgaggcg
     1801 ccgtccgagt ggacgaagtc gcgcaggctg ctgccgcccg cggcgagcgc gtcgcgcagc
     1861 gtttcccgga tcgagatcac cagacgctcg cattcggcgc gcgtcacccg ccgcgcgcga
     1921 cggcgcggat gcacgccggc ccggaacagg ctctcgttcg cgtagatatt gccgaccccg
     1981 acgacgatgt tggcgttcat gaggacctgc ttgatgcccg cctggcgccg ccgcgtcgcg
     2041 gcgtaaagat aatcggccga gaactcctcc gagagcggct cgacgcccag gcccgagagc
     2101 agccggtgga gccgcacgtc gcccggctgc cacagcaggg cgccgaagcg ccgcgggtcg
     2161 cgcaggcgca gcgccttgtc gccgaacacg aggtcgacgt ggtcgtgcgc gcttgccggc
     2221 gcgcgcggat cgatcacgcg caggctgccc gacatgccca ggtgcaggat gagggacccc
     2281 gagccgcact cgagcagcag atatttcccg cgccggtcga cgcgcttcac ctgctgaccg
     2341 atgatcgtgc gcgcgagcgt gcgcggcacg cgccagcgca ggttcgggtt gcggatcgcg
     2401 accccggtga tcgtgcggcc ggcgacgaag ggctcgatgc cgcggcgggt gatttcgact
     2461 tcgggcagtt cgggcatgga ttcgtgacgc gctcgggctt ttattattgt cgtcccatcg
     2521 aaatatacct aaactgtcgg ttccgcgaca gcaggcccac ctcatgtcct cccgcgtctt
     2581 gtgctccgtg ctacgcatgc gaatcccggt ccggggaatc gccgtggcgc tgctcgcggg
     2641 tgctctcgcc gcatgcgccg gacgggccgt gaagccgggc tccgacgacg atggcgggga
     2701 cgccgacgac ggcgacgcga gctcgttctt cgaaacgccg gagcgcggcg ttgcaccggc
     2761 gctgccgccg caggacctca cggaaacggt cctgtacgag ttcctgcttg ccgagatcgc
     2821 gacgcagcgc ggcgacgtca cgctggccgc gcaggcctat gtcgatctcg ccaagcgtac
     2881 gcgcgacccg cgcatcgcgc gccgcgccac cgaggtcgcg atgtacgcgc gcatggcgaa
     2941 cgccgctgtc gaggcggccc agatctggca ggagaccgag ccccagtcga cccgcgcgcg
     3001 ccaggcgctc gcgggactgc tggtcaacgc cggccgctac gacgaggcgc ttccgcattt
     3061 cgacaagctg ctcgccgacg gcgccgtgaa gcccgcggag accttctcgc agctcggacg
     3121 cgcgctcgcg agcgcccagg acaagaagtc ggcgctcaag ctcgtgcgca cgctcgcgca
     3181 gcgctatccg cgcgtgccgc aggcacactt cgccgtggcg cagaccgccg ccggcgccgg
     3241 cgacgaggag ctcgcgctcg ccgagatccg gcaggcggag caactgcgtc ccgattggga
     3301 caacgccgtg ctgctcgagg cgcagctgct gcagcgcaag tccccggacg ccgcggccgc
     3361 ccggctcgag accttcctga agagcaaccc gggctcgcgc gaagtgcgcc tggactacgc
     3421 acggctgctg gtctcgcaga aaaagatcgc cgaagcgcgc ggcgagttcc agaagctgct
     3481 cgccgcgtat ccggacaact cggacgtcac cttcgccgta gccctgctct ctctgcagct
     3541 caacgactac gacgccgccg aggcgaatct gaagcgcctg ctgcagggaa gctatcgcga
     3601 caagaacggc gtgcgcctgt acctcggcca gatcgccgag gatcgcaaga agctccccga
     3661 agcgctgcgc tggtacgacg agatcaccgg tggcgagcac tatctcgccg cccggatccg
     3721 gtccgcgcag gtcctgtcca agcagggcaa gctcgctgcc gcgcgcgagc ggctgcacca
     3781 gaccgagccg caaaacggtc agcagcgcat acagctcatt ctcgccgagg cgcagctcct
     3841 gcgcgacgcg aaccagccga aggccgcctt cgacatggtg cagcaggcgc tcgacaagct
     3901 gccgaaccag cccgacctgc tgtacgacca cgcgatgctg gccgagaagc tcgaccgcat
     3961 ggacatcctc gagtcgtcgc tgcgcaagct catcgcgctc aggcccgact acgcgcacgc
     4021 gtacaacgcg ctcggctact cgctcgctga ccgcaacgag cggctccccg aggcgcgcga
     4081 gctgatcgag aaggcgctga agctctcgcc ggacgactcc ttcatcatcg acagcatggg
     4141 ttgggtcctg taccgtcagg gaaagaccaa ggacgcgctc gagtggctgc agaaggcgta
     4201 tgccgcgcgg cccgacgccg agatcgccgc ccatctcggc gaggtgctgt gggtactcgg
     4261 tcgccgcgac gacgccgagc gtgtcctgcg cgacgcggcg cagaaggacc cgggcaacga
     4321 ggcgatcgcg agcactctca agcgcctgaa gcgctgagga cgcgggccgt gcggcgactg
     4381 gcgctcgtct gggtcgcggc ggtcgcgatc gccgcgtgcg cgacgctccc gaagcccgag
     4441 acgccgcagg gcgaaggcgc gttcgagctg cacggccggg tcgccgttcg ctacggctcc
     4501 gacggcgcct cgggcaacat cgcatggcgc cattcgccac aagccgacga ccttctcatc
     4561 acgtcgtttc tcggccaggg cgtggctcgg atccggcgct ccgacggcga ggtgcagctc
     4621 gtcgccgacg gcaaggagca ccgcgcgccc gacgccgaga ccttgaccga gcgcgtgctc
     4681 ggctggcggc tgccgctcga aggacttccc gactgggtcc aggggcatcc ggcgccgggg
     4741 cgcgctgagc agctgcgccg cgaaggcgag cgcctcgtcg cgttcgttca ggacgactgg
     4801 aaggtcgagt accaggcgtt cgacggggac cgtccctcgc ggctccggct gacgcggccg
     4861 aatctcgaga tccggctgat cgtcgatcag tggtcgggat gagcctctgg gaccgcgcct
     4921 ggccggcgcc cgcgaagctg aacctcttcc tgcacgtggt gggtcggcgg gaagacggct
     4981 atcacctcct gcagagcgcc ttcaggctga tcgaccgccg cgatcggctg cgctacgcgc
     5041 cccgcgacga cggccgtgtc gcgctgatgc ggccgcttcc cgggatcgac gaatcgaccg
     5101 agctctcggt gcgcgcggcg cggctgctcc tcgcggaatc gggctccagg cagggtgtgg
     5161 atatcgagat cgaaaagagc atccccgtgg gcggcgggct gggcggcggc agctccgacg
     5221 ccgcgaccac gctcgtggtc ctgaaccggc tgctgaggct cggcgcgtcc ccggaggacc
     5281 tcgcgcgcct cgggttgagc ctcggcgcgg atgttccgtt cttcgtcttc gggcggaacg
     5341 cgttcgccga aggcgtcggc gagcggctca cggcgctcgc gctgcccgcc gcttggtacg
     5401 ccgtgctcgt gccgccgctc gccgtgtcga cgaaggccat tttcgacgct cccgaattga
     5461 cacgaaattc aaaaaccatc aaaatatcaa gcttttcggc tggcttcggg cgtaacgacc
     5521 tggagcccgt cgtatgcagg cgccacgagg aagtcgcggc gcatctggct tggctgaagc
     5581 agttcggcga tgcgcgcttg agtgggtccg gcgcgtgcgt gttcgccgag tttccaagcg
     5641 aacgcgaggc gcgcgacgtt ttatccggca tgccggataa aatgaccggc ttcgtcgccc
     5701 gcgggctgga tcgacatccg ctcgcggatt tgctggacca tggttagcgc ttggggagtc
     5761 gccaagctgg ttaaggcagc ggattttgat tccgccatgc gagggttcga atccttcctc
     5821 cccagccagt ttttcggccc accgcgcagc cgcagattcg cggctgctcg catttccggg
     5881 gtagaagaaa gccgggaacc cgtcgcccgc agagacggta gccggtagag ggagacatgg
     5941 cgtacgaaag cctgatggtg ttcaccggca gcgccaaccc gctgctcgcg cacgcggtgg
     6001 tgcgccggcg caacatcccc ctgggccatg cgacggtggg cacgttctcg gacggcgaga
     6061 tcatggtcga ggtgctcgag aacgtgcgcg gcaaggactg cttcgtgctg cactcgacct
     6121 gcgcgccggc gaacgagagc ctaatggaag tgctgctgat ggtggacgcg ctgaagcgcg
     6181 cctccgcggc gcgggtgacg gcggcgatcc cgtacttcgg gtacgcgcgc caggaccggc
     6241 gtccgcgctc ggcgcgcgtg gcgatcagcg ccaaggtagt ggcgaacatg ctgcaggccg
     6301 cgggcgtaga ccgcgtgctg acgatggact tgcacgccga ccagatccag ggatttttcg
     6361 acgtgccggt ggacaacatc tatgccacgc cggtgctgct cggagacgta tggaagcatc
     6421 agtacgagaa cctgctggtt gtgtcgcccg acgtcggcgg cgtggtgcgg gcgcgtgcga
     6481 tggcgaagcg gctggagtcg gacctggcga tcatcgacaa gcgccggccg cagccgaacg
     6541 tgtcggaagt gatgaacgtc atcggcgacg tgaagggcag gacctgcgtg atcatggacg
     6601 acatcgtcga caccgcgaac acgctgtgca aggccgcgga ggcgctgaag aacgagggcg
     6661 cgacgcgcgt cgtcgcgtac tgcacgcacc cggtgctctc ggggggcgcg gtcgagcgca
     6721 tcgcggcctc gtcgatggac gagctggtgg tggccgacac catcccgttg tcgaaggcgg
     6781 ggcaggcgtg caagaagatc cgcgtgctct cggtcgccgg gctgctcgcg gagacgatat
     6841 tgcggatcag caacgaggac tcggtgagct cgttgttcat ggaatgattt tttgaacggt
     6901 cgccgcctgg tcgcgggtgg cgtcccactt aggagaagcc aatggcaatc gaagtccaag
     6961 cgcaagcgcg caagctgcag ggaacgggtg cgagccgccg cctgcgccgc gaaggcaagg
     7021 tccccgggat cgtctacgga gggagccagc ccgcgctcac catcgagctc gatcacaacg
     7081 cgctgttcca ccacctgcgc aacgagaagt tccatgcgtc catcctgacg ctgaaagtgg
     7141 acggcgccac cgagcaggtg ctgctgcgcg cggtgaacat gcatccgttc aagctgcagg
     7201 tccagcacat cgacttccag cgggtctcgg cgaaagagaa gatccacatg aaggtgccgc
     7261 tgcacttcgt gaacgccgag gcctcgcccg cggtgaaggt ctcgggcggc atcatcagcc
     7321 acgtcgcgaa cgagctcgac gtgcgctgcc tcccggccga cctgcccgag ttcatcgagg
     7381 tcgatctctc gaagctcgag atcaacgact cgatccacgt gcgcgatttg gcgctgccga
     7441 agggcgtcga gctgatgctg cgcggccagg agaacccgcc ggtcgcgacg gcgcaagtgc
     7501 cgaaggccgc gatcgaggag gaagaggccg ccgcagccgc ggctgccgcg accgccgagg
     7561 gcaccgctgc gcccgaggcg actccgtccg ccgccgatgt ccccgcagtc aagcagaagg
     7621 cgcccgaagc cgccgccccc gctgccgagg acaagggcaa aggcaagagc gagaagaagt
     7681 agggtcgatc cggcgcgcga ggaagggcgc gacgaacgcg ccctttttca tctatggctt
     7741 ccatcaggct gatcgcaggt ctgggcaacc ccgggcgcga gcatgatcgc gaccggcaca
     7801 acgtcggctt catgttcgca tcgcgcctcg ccgccgcgag ccgggtggac atgagatcgt
     7861 cgtcccggta cgcgggcctg ctcggaaaga tcgcgcaccc ggccggggac atctggctgc
     7921 tcatgccgca gacgtacatg aaccggtcgg gcgaatcggt cgggccgctc gttcacttct
     7981 acaagatccg gccgcaggaa gttctggtcg tgcacgacga gctcgatttc ccgcccggtg
     8041 tggtcaagat caagctcggc ggcggcgtag cgggccacaa cggcctaaag gacattgccg
     8101 cgcgtctcgg ctcgcacgat ttctggcgcc tgcgcatcgg tatcggccat cccggtgaca
     8161 aggccgtcgt cccggattac gtgctcaagt cgccgtcccc cgaggaccgc gagcagatcg
     8221 agcgggcgct cgagcgcagt ctcgaggtcg cgccgctcat gctggacggc aagatggaag
     8281 cggcgatgct gaagctgcac acgaaggccg cctgagatgt ccgtccaatg cggaatcgtc
     8341 ggcttgccca atgtcggcaa gtccaccttg ttcaacgcgc tgaccaaggc ggggatcgcg
     8401 gcagagaatt acccgttctg cacgatcgag ccgaacgtcg gcatcgtcga ggtgccggac
     8461 ccgagactgg cgaggctcgc ggacatcgcg cagccgcaga agatcgtacc ggcggtcgtc
     8521 gaattcgtcg atatcgcggg cctcgtcgcg ggcgcgtcga agggcgaggg acttggcaac
     8581 cagttcctcg ccaacatccg cgagtgcgac gcgatcgcgc acgtggtgcg ctgtttcgag
     8641 gatgcgaacg tcgtgcacgt ggcgggcaag gtcgacccgg tgtccgacat cgagaccatc
     8701 aacaccgagc tcgcgctcgc cgatctcgcg gcggtcgaga agcagctcgc caagaaccag
     8761 aagctcgcgc gcgccggcgg cgacaaggag gcccagcgcc tggtcgcggc gctcgaaaag
     8821 gtccaggccg cgctcgacca gggcaagccc gcgcgcggaa ttccgctgtc gaaggaggag
     8881 ctggcggtga tcaggccgct gttcctgctg accatgaagc cgacgatgta cgtcgccaac
     8941 gtgtccgagc acggcttcgc cggaaatccg ctgctcgaga aagtgcaggt atatgccaag
     9001 gccgagggcg ctcccgtggt cgcgatctcg gctgcgctcg aggcccagat cgccgatctg
     9061 tcggacgagg acaagaaggt cttcctcgag gacatggggc tcgaggagcc cgggctggat
     9121 cgcctgatcc gggccgggta cgccctgctc gggctgcaga cctacttcac cgcgggcccc
     9181 aaggaggtgc gcgcctggac gattccggcc ggcgcgacgg cgccgcaggc ggcgggcgtc
     9241 atccacaccg atttcgagcg tggcttcatc cgcgccgagg tcgccgcgta cgacgattac
     9301 gtcggctgca aaggcgagca aggcgcgcgc gaggcgggga agctgaggct cgaaggcaag
     9361 gactacgtcg tgcgcgacgg tgatgtggtg cacttcaggt tcaacgtctg acgctcgggg
     9421 cggcgtcagc gcccgtcgat catgtagtgc gtccacgccc gccattcctg cgcggagcgc
     9481 agcaccgcgg acggcagggc cattcctttg aaggtgtagg tgcgcggatc cgcgatcttg
     9541 tgcttggcga cgaaccggcg cgcatgatcc agccaggtgc acgagaactt tccggccgac
     9601 tggtgcacga gcaggatgtc gcccgcgacg ccgacggaaa atccggcgac ggccgccgaa
     9661 tagctgaagt ccaggtcgta gagatgccag tcgtcgaacg tgcgctcgtc gaacccgatc
     9721 ttctcggcga ccgcgcgccg gcacgcgaag aacacgccgt cgagcgcctg catcgcgccc
     9781 gcgctcctct ccgacagcag ataggcggtc gtcacgatcc cgcggccgcc gcgctcgggc
     9841 atgccgatct gcccgtggac gtacggccag ccggcatcga tccagctgcc gccgcaaacg
     9901 cggtcggtcc cggccacgcc gaccagcgcg aaatccgaaa gccggttcgc gagcttggcc
     9961 gcgaagtccg gcgcgacaat ctcgatgtcg tcgtgagaga acacgaggat ctctccgcgc
    10021 gcgcggcgta ctccgcggtt gtagccctcg gccagcgatc gcgcgtcgtg cacgcccacg
    10081 atctcgtgat cgaccccggc gagcagcgcc gcgtagttcg ccgtgacccg ctcgtatttc
    10141 gacggcgtga tgctgcagac gatgacgctg atcgtaggcg ccgcgccgcg cag
//
LOCUS       DATDMW010000399        12002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_989455, whole genome shotgun sequence.
ACCESSION   DATDMW010000399 DATDMW010000000
VERSION     DATDMW010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12002
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989455"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VI319_16600"
     CDS             <1..280
                     /locus_tag="VI319_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865514.1"
                     /translation="GDLIALAPCRQPGGKLVLAFALEGDCDAGAARSNLFSMEWPPRS
                     GRMREFPEVDRAAWFGIDEARRRIMPGQLPLIEELERMLDERSLHGAR"
     gene            267..965
                     /locus_tag="VI319_16605"
     CDS             267..965
                     /locus_tag="VI319_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6865515.1"
                     /translation="MARDETALIVGAGSGLSAALARALAAEGVRVALAARNAGKLEAL
                     ARETGALALACDAVERAQVEAAFAAVEKKWSAPDLVVYNASYRTRGPVVELDPPEVKR
                     SLEVSAFGGFLVAQAAAKRMLAKQSGAIFFTGASASVKGYAESAPFAMGKFALRGLAQ
                     SLARELAPKGIHVAHFVIDGGIGRADDPRAAERGPDGLLAPDAIASTYLHVWRQHRSA
                     WTWEVELRPWVERF"
     gene            975..1370
                     /locus_tag="VI319_16610"
     CDS             975..1370
                     /locus_tag="VI319_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6865516.1"
                     /translation="MGLRVLVVDDDRDLATGTEMILRAMGHDVRVAHDGAEALEIAAA
                     FQPEAALVDLGMPNMNGYLLARKLREWRWTRDAVLIAVTGWGGERERERAKEAGFDHH
                     LLKPAQPERIAELLAEVRPAASAARGDNP"
     gene            1401..2144
                     /locus_tag="VI319_16615"
     CDS             1401..2144
                     /locus_tag="VI319_16615"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY6865517.1"
                     /translation="MAGKNGSVVARLDRVVDDFSKRARFFREPLTRGRARMFVLQHRL
                     NTRQRNSVLKLRVATNCPDWDTRIGIIGACSQEVIADHEHGGGRAHWQILEDLGTKIG
                     MKRGDIRAAKPLASTQIAWAAWEGLMSNRHWLEGVVANVCAERANVPGYGTGTMKKHG
                     WFGLERERWGALFGLADADLEFFELHEEADIEHSDMGWRAIERFAKQLKMEDAVVEAC
                     ERNLQVWEMYLDGIAAGGDRMAAGRDRLG"
     gene            complement(2164..2541)
                     /locus_tag="VI319_16620"
     CDS             complement(2164..2541)
                     /locus_tag="VI319_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865518.1"
                     /translation="MRLAPEDDVEALLQPQVRARLPFSRLLLIYLNPFALFKDVSRGN
                     VYAQRVALAYNRAMRWMLLRYLRRWTTIAATLFVSIAPAEALAAQAPVFVVSAAASAV
                     GCCIAVTVIVCTVATYLMLGRRD"
     gene            complement(2574..3545)
                     /locus_tag="VI319_16625"
     CDS             complement(2574..3545)
                     /locus_tag="VI319_16625"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865519.1"
                     /translation="MSPWTRAGVTLAFAALLAAGVAHAQQYPSKPVRMLVGFAPGGGV
                     DITARLVSQKLTERWGKPVVVENRAGAAGNIATEIVAKSAPDGHTLLMAFSSHASNAA
                     LYEKLPFDITRDFTPVSLVATAPVVIAASASLPVKSLSELIAYARSRPGAVRFASSGV
                     GTPVQLAGELMMQLTGTRMTHVPYKGIAPAMAAMLGGETDVTFPAVISGMQHFRSGRL
                     KALAIASAMRYPGLPDVPTAAEAGLAGFEVDYWYAVFGPAGLPRPVADRLTQEITAIV
                     TTPAMKQSMLEQGSIAVGGGPGDLDALVRREYALWSKVVKAGNVKAE"
     gene            complement(3542..5209)
                     /locus_tag="VI319_16630"
     CDS             complement(3542..5209)
                     /locus_tag="VI319_16630"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY6865520.1"
                     /translation="MSKARLFAWIAVVILPFVSPLAAAQAPALAGTVTSAAEGAMEGV
                     LVSAKKDGSTITITVVSDARGRYAFPAAKLAPGRYALGIRAVGYELASPVSAQVNPVE
                     TARADLRLAKAKDLAAQLSNGEWIASFPGTDAQKGLLLNCVGCHTVERIARSTYDADT
                     FMKVLLPRMQGYVNQSIPQHPQLRRAERLMEERGDQRVQVYRSTAEYLATINRSAGEW
                     KYALKTHARPSGRATRVVYTEYALPRETIEPHDVIVDSDGIAWYSSFGEQYLGRLDPK
                     TGAVREFLIEVNKPGFPTGLLGLRSDRDGNLWLGNMYQATIVRFDRKAQAFTTWQLPA
                     ERNIDAAQINMVSPQFAHVDGKVWTQNNGFAGVHRLDVKTGKIETWEPFKGAPKGEPH
                     NIYDVVPDSQNNAWFTDFRWRHIGRIDAKTGEVKLYEVPATAKVVAPRRGMMDASDRL
                     WFAQYRGDKIAVLDTKTGQFRDWAVAPKWSSPYDVTIDRNEEAWTGSMVTDFVTRVDT
                     KTGRSVNYLLPHKTNIRRVFVDNSTTPVTFWVGSNHGAAIVKLEPLD"
     gene            complement(5304..7769)
                     /locus_tag="VI319_16635"
     CDS             complement(5304..7769)
                     /locus_tag="VI319_16635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6865521.1"
                     /translation="MNGPSKGPRRGGEIAIAATLIGVGALVAVSWIVRLRFPLEIVPG
                     FAPMVLGTALCFVVAGGWLWALERSRTAAGVLAALLTAFAVAVIAQYALGLNLRIDAD
                     SVHRWLLPSAPHPGRTSAFTALALAMAGVAMLLSLLPSRPAVDTAGVVLACGPALVGV
                     SVLIGYLIELHLVYAGYALGVVAMHTAIGLIALSVGLWLSLQRRGRVPFWPRLRPEDR
                     ITFASATVLGLVSLAVAVGVFAAMQGQLEKALSEGLEMNLRSRVAVAEGAIEAGVVRA
                     RNLAGRPAPARALRSLAAPAGSAEARAQLQQSASSLVADGDVAAAYYDASGREVASAG
                     ERITDAELAIPLAGSAGVELAWSGRFIVSARYPMMDARGEAGAALVQTPAPALDSLLR
                     NSSGLGATGEVGLCALLQDRLGCFPQLRVPHVYYAAARTPQGVSFPMARAAAGEAGVV
                     LTRDYRGQNVLAAFAPVGRFGLAMVVKMDTAEVYAPIRERLGFVLPLLAGLTALGTLL
                     LRAGVRPLAAQLARSEDAARAQYRAIEGVMASVADGMMTLDRDGTIRSWNAAAEKMFG
                     YATAEVVGRNLSLLVPDTLRGRNMAATRRYLQTGLSPVIGRSNVNYPARRKDGSEFQL
                     EFTVTETLGAAGPQLVAVFRDITERKRAEERLTALALRDALTGLANREHFNRRLEEAF
                     ARRRRGAGEVALLFLDLDRFKLVNDTLGHEVGDGLLGAFAARLGAAVRETDLVARLGG
                     DEFTVVLEGLTDPADAAGVAEKVVAAMRVPIEVGTHAMTASVSIGIAVCREGDTPRML
                     MRRADAALYEAKRAGRARYHVAT"
     gene            complement(7858..8571)
                     /locus_tag="VI319_16640"
     CDS             complement(7858..8571)
                     /locus_tag="VI319_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865522.1"
                     /translation="MNLSDSLLAAFIGAGATVTGALVQLRIAWRKEMRERERGQPVSR
                     KQRRGPVMLVFALMIASAVGGFALSQYLMSLRYADQDAIRAELQAKLNEIAANAARLE
                     HARIGESPAGDTRAKPAEPAPDEGATGSIVVPPCKAAGDAKPDCSEQGAARVAVCARV
                     PAAASVKEVLLYTKAEDSPQPWEAARVQPGQDAGQAKFVEKFYERADADGSKQVCQSF
                     ASWNAEKARAARILVKYSL"
     gene            8743..9660
                     /locus_tag="VI319_16645"
     CDS             8743..9660
                     /locus_tag="VI319_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY6865523.1"
                     /translation="MKRPSLYSAARPRAAPPAEPRAAAPSADPAPAPRVSRLESFGSR
                     HQRPLLVAAGALLAALLVALHAAMTPAPRHITQRDIDNAVRHSLETHPLPSRAAQAYE
                     AVAPAIVRVQGVRKAEAVADGAAPSEGSTGSGVVVHESGVILTSLHVIEGTDSIAVDF
                     ATGEHSEAEVIAVQPENDLAVLRARTLPDDLVPATLGAAADLKPGDDVIAVGFPFGIG
                     PSVTSGVISGLNREYQAPDGGRSLSRLIQFDAAANPGSSGGPLVTATGEVVGIVTGIL
                     NPSDQRVFAGIAFAVPIETASSAVGLPPF"
     gene            9668..10654
                     /locus_tag="VI319_16650"
     CDS             9668..10654
                     /locus_tag="VI319_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6865524.1"
                     /translation="MSSSLLERILYEVKKVVVGQDHFLERVLVAILAQGHLLVEGVPG
                     LAKTLTVKTLARTIRGSFRRIQFTPDLVPSDLVGTRIYNQRTGDFATSLGPVFANLLL
                     ADEINRAPAKVQSALLEVMQEYQVTIAGETHHVPEPFLVMATQNPIETEGTYPLPEAQ
                     VDRFMMKVLVGYPSEEEEFVIVNRVTGPSVDVAPVATTEQLAELQRECRRSYVDPALI
                     QYAVRLAGATRDPERHGIPDLAHYISYGASPRASIHLIEAGRALAFLRGRDYLLPQDV
                     TDVTPDVLRHRVVLSYEALANGVTPDEIVQRVLEHVRAPAKPMERHVQAAAG"
     gene            10632..11564
                     /locus_tag="VI319_16655"
     CDS             10632..11564
                     /locus_tag="VI319_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY6865525.1"
                     /translation="MSRPPQAEAVLRRLEWTVIRRLDGLLHGDYRTLFRGFGLDLADL
                     REYQYHDDVRRIDWNVTARLGTPYVREYHEDREVTAWFLLDLSPSVDFGSRTRKRSVS
                     AEFVAVLARLLTRHGNSVGAMFYGDRLDAVIPPRSSRRHVLHLLEAMRARPPLAASRP
                     TDLRAFLRSAFGMLGRRSVVFVVSDFISAPGWAEPLALLAQSHDVLAVRLHDVLESEM
                     PDLGLLVVQDAETGEQLFVDTHDRGFRRRFARAAEAREGAVRAAFAQAGVDALELSTD
                     GDLVDAVLRFADLRKRRVRRPAGAPAPLRAGVTA"
     gene            11561..>12002
                     /locus_tag="VI319_16660"
     CDS             11561..>12002
                     /locus_tag="VI319_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6865526.1"
                     /translation="MTFLWPDLLWLLAAVPLLVAAYVLALRRRKKAALRYASLEVVKA
                     AMGPGQSVRRHVPPALFLAALVLLIVAVARPAAVVKLPSDDKTIILAIDVSGSMRAED
                     VQPNRLAAAQAAARQFVSDQPSGTKVGLVAFAGTAALVQPPTRGR"
BASE COUNT         1731 a         4304 c         4258 g         1709 t
ORIGIN      
        1 gggcgatctg atcgcgctcg cgccatgccg gcagccgggc ggcaagctgg tcctcgcctt
       61 cgccctcgaa ggcgactgcg acgcgggcgc cgcgcgcagc aacctgttca gcatggaatg
      121 gccgccgcgt tccggccgga tgcgggagtt cccggaggtc gaccgcgccg cctggttcgg
      181 gatcgacgag gcgcggcgca ggatcatgcc gggccagctg ccgctgatcg aagagctcga
      241 gcgcatgctc gacgaaagga gccttcatgg cgcgcgatga gacggcgctg atcgtgggcg
      301 cggggagcgg cctctccgcc gcgctcgcgc gggcgctcgc cgccgagggc gtgcgcgtgg
      361 cgctcgccgc gcgcaacgcc ggcaagctcg aggcgctcgc gcgcgagacg ggcgcgctcg
      421 cgctcgcctg cgacgcggtc gagcgcgccc aggtcgaggc cgcgttcgcc gcggtggaga
      481 agaagtggtc ggcgcccgac ctggtcgtgt acaacgcgag ctaccgcacg cgcggtcccg
      541 tcgtcgagct cgacccgccc gaggtcaagc gctcgctcga ggtcagcgcg ttcgggggct
      601 tcctggtcgc gcaagccgcc gcgaagcgca tgctggcgaa gcagagcggc gcgatcttct
      661 tcaccggcgc ctcggcgagc gtcaagggct acgccgagtc ggcgccgttc gcgatgggga
      721 agttcgcgct gcgcggcctc gcgcagagcc tggcgcgcga gctcgcgccg aagggcatcc
      781 acgtcgcgca cttcgtgatc gacggcggca tcggccgcgc cgacgacccg cgcgccgccg
      841 agcgcggtcc cgatggcctg ctcgcgcccg acgcgatcgc gagcacctat ctgcacgtct
      901 ggcgccagca ccgcagcgcc tggacctggg aggtggagtt gcgcccctgg gtcgagcgct
      961 tctgaaggag cgccatggga ctgcgcgtgc tggtggtgga cgacgaccgg gacctcgcga
     1021 cggggaccga gatgatcctg cgcgcgatgg gccacgacgt gcgcgtcgcc cacgacggcg
     1081 ccgaggcgct cgagatcgcc gccgcgttcc agcccgaggc ggcgctggtc gacctcggca
     1141 tgcccaacat gaacggctac ctgctcgcgc gcaagctgcg cgaatggcgc tggacgcgcg
     1201 acgccgtcct gattgcggtc accggctggg gcggcgagcg cgagcgcgag cgcgcgaaag
     1261 aggcgggctt cgatcaccac ctgctgaagc cggcgcagcc cgagagaatc gccgagctgc
     1321 tcgccgaggt gcgtccggcg gcgagcgcgg cgcgcggcga taatccataa tcacggcttt
     1381 ttcgcggggt cggcaaggca atggcgggaa agaacggcag cgtggtggcg cgtctcgatc
     1441 gcgtcgtgga cgatttctcg aagcgcgcgc gcttcttccg cgagccgctg acgcgcggac
     1501 gcgcgcgcat gttcgtgctg cagcaccggc tgaacacgcg ccagcgaaat tcggttctca
     1561 agctcagggt cgcgaccaac tgcccggact gggacacgcg catcggcatc atcggcgcgt
     1621 gcagccagga ggtgatcgcc gaccacgagc acggcggcgg gcgcgcgcac tggcagatcc
     1681 tcgaggacct cggcacgaag atcggcatga agcgcggcga catccgcgcc gcgaagccgc
     1741 tcgcctcgac gcagatcgcg tgggccgcgt gggaggggct gatgagcaac cgccactggc
     1801 tcgagggcgt ggtcgcgaac gtctgcgccg agcgcgccaa cgtcccgggc tacggcaccg
     1861 gcaccatgaa gaagcacggc tggttcgggc tcgagcgcga gcgctggggc gcgctgttcg
     1921 ggctcgcgga cgccgatctc gagttcttcg agctgcacga ggaggccgac atcgagcact
     1981 ccgacatggg ctggcgcgcg atcgagcgct tcgcgaagca gctgaagatg gaggacgccg
     2041 tggtcgaggc gtgcgagcgc aacctgcagg tctgggagat gtatctcgac ggcatcgccg
     2101 cgggcggcga tcgtatggcc gcgggccgcg atcgtctggg ctgaaagcga gcgcgggacg
     2161 cgcctagtcc cggcgtccca gcatcaggta ggtggcgacc gtgcacacga tcaccgtgac
     2221 ggcgatgcag cagccgaccg ccgacgccgc ggcggagacc acgaacaccg gcgcctgcgc
     2281 cgcgagcgcc tccgccggcg cgatcgacac gaacagcgtc gccgcgatcg tcgtccagcg
     2341 gcgcaggtag cgcagcagca tccagcgcat cgcgcggttg tacgcgagcg cgacgcgctg
     2401 cgcgtacacg ttcccgcgcg acacgtcctt gaatagcgcg aacggattaa ggtagatcag
     2461 gagcagccgc gaaaacggaa gccgcgcgcg cacctgcggc tgcagcagcg cttcgacgtc
     2521 gtcttccggg gcgagtcgca tgctccttag tcaatcgtcc ggggaatcga gtttcactcg
     2581 gccttgacgt tgcccgcctt caccaccttc gaccagagcg cgtattcgcg gcgcacgagc
     2641 gcgtccagat cgccaggacc tccgccgacc gcgatcgatc cctgctcgag catgctttgc
     2701 ttcatcgcgg gcgtggtgac gatcgccgtg atctcttgcg taagacggtc cgccaccggg
     2761 cgcggcagcc cggcgggacc gaagaccgcg taccagtagt cgacctcgaa gcctgcgagc
     2821 ccggcctcgg ccgcggtcgg cacgtcgggc aggccggggt agcgcatcgc gctcgcgatc
     2881 gcgagcgcct tcaggcgccc gctcctgaaa tgctgcatgc ccgagatcac cgccggaaag
     2941 gtgacgtcgg tctcgccgcc gagcatcgcg gccatcgcgg gcgcgatgcc cttgtacggc
     3001 acgtgcgtca tgcgggtccc ggtgagctgc atcatcagct cgcccgcgag ctgcaccggc
     3061 gtgccgacgc ccgaggaggc gaagcgcacg gcacccgggc gcgatctcgc gtacgcgatc
     3121 agctcggaga gactcttcac cggcagcgac gcgctcgccg cgatcaccac cggtgccgtc
     3181 gccaccagcg acaccggcgt gaaatcgcgc gtgatgtcga agggcagctt ctcgtacagc
     3241 gcggcgttgc tcgcgtgcga cgagaacgcc atgaggagcg tgtggccgtc gggcgcgctc
     3301 ttcgccacga tctcggtggc aatgttgccc gcggcgccgg cgcggttctc gaccaccacg
     3361 ggcttgcccc agcgctccgt gagcttctgc gacaccaggc gcgcggtgat gtcgacgccg
     3421 ccgccggggg cgaagccgac cagcatgcgc acgggcttgg acggatattg ctgcgcgtgc
     3481 gcgacgccgg ccgcgagcag cgcggcgaac gccagcgtga cgccggctct ggtccagggg
     3541 ctcaatcgag cggctccagc ttgacgatcg ccgcgccgtg gttgctgccg acccagaagg
     3601 tcaccggcgt cgtggagttg tcgacgaaga cacggcggat gttggtcttg tgcggcagca
     3661 ggtagttgac cgaccggccg gtcttcgtat cgacgcgggt cacgaagtcg gtgaccatcg
     3721 atccggtcca cgcctcctcg ttgcggtcga tcgtgacgtc gtagggcgag gaccacttcg
     3781 gcgcgaccgc ccagtcgcgg aactgcccgg tcttcgtgtc gagcaccgcg atcttgtcgc
     3841 cgcggtactg cgcgaaccag agacgatcgc tcgcatccat catcccgcgg cgaggagcga
     3901 cgaccttggc ggtggccggg acctcgtaga gcttcacttc gccggttttc gcgtcgatgc
     3961 gcccgatgtg gcgccagcgg aagtcggtga accaggcgtt gttctgcgag tcgggaacga
     4021 catcgtagat gttgtgcggc tcgcccttgg gcgcgccctt gaacggctcc caggtctcga
     4081 tctttccggt cttcacgtcg aggcggtgca cgccggcgaa gccgttgttc tgcgtccaca
     4141 ccttgccgtc gacgtgcgcg aactgcgggc tcaccatgtt gatctgcgcg gcgtcgatgt
     4201 tccgctcggc cggcagctgc caggtggtga acgcctgcgc cttgcgatcg aaccggacga
     4261 tggtcgcctg gtacatgttc ccgagccaca ggttgccgtc gcggtcggag cgcaggccga
     4321 gcagtccggt cgggaagccg ggcttgttca cctcgatcag gaactcgcgc accgcgccgg
     4381 tcttcgggtc caggcgcccg aggtactgct cgccgaagct cgagtaccag gcgatgccgt
     4441 cggaatcgac gatgacgtcg tgcggctcga tcgtttcgcg cggcagcgcg tactcggtgt
     4501 agacgacgcg cgtcgcgcgg ccgctcgggc gcgcgtgggt cttcagcgcg tatttccatt
     4561 cgccggcgct gcggttgatg gtggcgaggt actcggcggt cgagcggtag acctgcacgc
     4621 gctggtctcc gcgctcttcc atcaggcgct cggcgcgccg caattgcggg tgctgcggga
     4681 tgctctggtt cacgtagccc tgcatgcgcg ggaggagcac cttcatgaac gtgtccgcgt
     4741 cgtaggtcga gcgcgcgatg cgctccaccg tgtggcagcc gacgcagttg agcagcagcc
     4801 ccttctgcgc gtccgtgccc gggaagctcg cgatccattc gccgttcgag agctgcgcgg
     4861 cgaggtcctt ggccttcgcc aggcgcaggt cggcgcgcgc cgtctcgacc gggttcacct
     4921 gcgccgacac cggggacgcg agctcgtagc cgaccgcgcg gatgccgagc gcgtacctcc
     4981 cgggcgcgag cttcgccgcc gggaaggcgt agcgcccgcg cgcatcgctg acgacggtga
     5041 tggtgatggt ggagccgtcc ttcttcgcac tgacgagcac gccctccatc gcgccttccg
     5101 cggcggaggt gacggtgccg gcgagcgcgg gcgcctgggc ggcggcgagc ggcgagacga
     5161 agggaaggat gacgaccgcg atccaggcga aaagccgggc cttgctcatc gcaacctcct
     5221 gggggaagga acgtcgtgcg gcaggacgcc cccatcatag cagcggcccg ggcgtgcagg
     5281 ttttccctgg gatggtcgag cagctaggtc gccacgtggt agcgcgcacg cccggcgcgc
     5341 tttgcctcgt agagcgcggc gtccgcgcgg cgcatgagca tccgcggcgt gtcgccctcg
     5401 cggcacacgg cgatgccgat gctgacggag gcggtcatcg catgcgtgcc gacctcgatc
     5461 gggacgcgca tcgcggcgac caccttctcg gcgacgcccg cggcgtccgc gggatcagtg
     5521 agcccttcca ggaccaccgt gaactcgtct ccgccgagcc tggcgacgag gtcggtctcg
     5581 cgcacggccg cgcccagtcg cgccgcgaac gcgcccagga ggccgtcgcc gacctcgtgg
     5641 ccgagggtgt cattgacgag cttgaacctg tccaggtcga ggaacagcaa cgccacctcg
     5701 ccggcgccgc ggcggcggcg cgcgaaggcc tcctccagcc ggcggttgaa gtgctctcga
     5761 ttggcgaggc cggtgagcgc gtcgcgcagc gcgagcgccg tcaaccgctc ctcggcgcgc
     5821 ttgcgctcgg tgatgtcgcg gaacaccgcg accaactgcg gcccggccgc gccgagggtc
     5881 tcggtgacgg tgaactcgag ctggaactcc gagccgtcct tgcggcgggc gggatagttc
     5941 acgttgctgc ggccgatcac cgggctcagg ccggtctgca ggtagcggcg tgtcgccgcc
     6001 atgttccgcc cgcgcagggt gtccgggacc agcagcgaca gattgcggcc gacgacctcg
     6061 gccgtggcgt agccgaacat cttctcggcg gcggcgttcc acgagcggat ggtcccgtcg
     6121 cggtccagag tcatcatgcc gtcggcgacg ctcgccatca cgccttcgat ggcgcggtat
     6181 tgcgcgcggg cagcgtcctc cgagcgcgcg agctgcgcag cgagcggccg cacgccggcg
     6241 cgcagcagca gcgtgccgag ggccgtcagg ccggcgagca gcggcagcac gaatccgagg
     6301 cgctcccgaa tcggcgcgta cacctcggcg gtgtccatct tcaccaccat ggcgaggccg
     6361 aagcggccca ccggcgcgaa tgccgccagc acgttctggc cgcgatagtc gcgcgtgagc
     6421 acgacgccag cctcaccggc ggccgctcgc gccatcggaa acgacacccc ttggggagtt
     6481 ctcgccgcgg cgtagtacac gtgcggcacg cgcaactgcg gaaagcagcc gagccggtcc
     6541 tgaaggagcg cacacaggcc cacctcgccg gtcgcgccga gacccgagga gttccggagc
     6601 agcgagtcga gcgcgggtgc cggcgtctgc acgagcgcgg cgccggcttc gccgcgcgcg
     6661 tccatcatcg gatagcgagc cgaaacgatg aagcggccgc tccaggctag ctcgaccccg
     6721 gcgctgccgg ccagtgggat ggcgagctcg gcatcggtga tccgctcgcc ggccgaggcc
     6781 acctcgcggc cgctcgcgtc gtagtaagcc gccgcgacgt cgccgtcggc caccaggctc
     6841 gacgcgctct gctgcagctg cgcccgtgcc tccgcggagc ccgcgggggc cgcgaggctt
     6901 cgcagcgcgc gcgccggcgc agggcgtccc gcgaggttgc gcgcacggac gacgccggct
     6961 tcgatcgcgc cttccgcgac cgcaacgcgc gagcgcaggt tcatctcgag cccctcgctc
     7021 agcgcctttt ccagctgccc ctgcatggct gcgaacacgc ccaccgccac ggccaacgac
     7081 accaggccga ggaccgtcgc ggacgcgaac gtaatacggt cctcgggacg aagccgcggc
     7141 cagaaaggca ctctcccgcg acgctggagc gagagccaca gtccaacgga gagcgcgatg
     7201 aggccgatcg cggtatgcat cgccacgacc ccgagcgcgt acccggcata gacgaggtgc
     7261 agctcgatga gatagccgat caggaccgac acgccgacca gggccgggcc gcaggccagc
     7321 acgacacccg ccgtgtcgac cgccgggcgc gaaggcagga ggctgagcag catggcgacg
     7381 cccgccatcg caagcgcgag cgccgtgaac gccgacgtgc gcccggggtg gggcgcgctc
     7441 gggagcagcc agcgatggac cgagtccgcg tcgatgcgga gattcaaccc gagcgcgtat
     7501 tgcgcaatga cggcgacggc gaaagctgtc aacagcgccg ccaggacgcc cgccgccgtg
     7561 cggctccgct cgagcgccca gagccatccc ccagcgacga cgaagcagag cgccgtgccg
     7621 agcaccatgg gagcgaaccc gggaacgatc tcgagcggaa agcgcaggcg cacgatccag
     7681 gaaacggcca cgagggcgcc gacgccgatc agcgtcgcgg cgatggcgat ctcgcctcct
     7741 cggcggggcc ctttgctcgg accgttcatt gagttgttcc cgacgcccga gaggcgccgg
     7801 tcgccacgat gatggagcgc cgccgcgcgg gcgtcaatcc cggctaggcg gccggcgcta
     7861 cagcgagtac ttgaccagga tgcgcgccgc gcgcgccttc tcggcgttcc agcttgcgaa
     7921 ggactgacag acctgcttgc tgccgtccgc gtcggcgcgc tcgtagaact tctcgacgaa
     7981 tttggcttgg ccggcgtcct gcccgggctg cacgcgcgcc gcttcccagg gctgcggcga
     8041 gtcctccgcc ttcgtataca gcaggacctc cttgacgctc gcagcggccg gcacgcgcgc
     8101 gcacaccgcc acacgcgccg cgccctgctc gctgcagtcg ggcttggcgt cacccgccgc
     8161 cttgcacggc ggcacgacga tgctgccggt cgcgccctcg tccggcgcgg gctcggcggg
     8221 cttcgcgcga gtgtcgcccg ccggcgactc gccgatgcgc gcgtgctcga ggcgcgccgc
     8281 gttcgccgcg atctcgttca gcttcgcctg cagctccgcg cgaatcgcgt cctggtccgc
     8341 gtagcgcagg ctcatcaggt attgcgaaag cgcgaagccg cccaccgccg aggcgatcat
     8401 cagcgcgaac acgagcatca ccgggccacg ccgctgcttc ctcgagaccg gctgaccgcg
     8461 ctcgcgctcg cgcatctcct tgcgccaggc gatgcgcagc tgcaccagcg cgcccgtgac
     8521 ggtcgccccg gcgccgatga aggccgcgag gagtgagtcg gaaagattca tgcgcagaat
     8581 catacgggat cgccgggaga cgcaggacgc cacccgccgg atggcgtgaa acgtgtcacg
     8641 cggctgccac gcaaccatcc ggaggcgcca aggtctagca ggaaatcgcc acgcgcggcg
     8701 ggccgcgcgc ggtccgcatc acctggcctt atccggtcct aaatgaagcg accctcgctg
     8761 tacagcgccg cgcggccgcg cgcggctcca cccgccgagc ctcgcgctgc tgcgccgtcc
     8821 gcggacccgg cgcccgcccc gcgcgtctcg cgactcgaat cgttcggatc ccgccatcag
     8881 cggccgctgc tggtcgccgc cggcgcgctg ctcgccgcgc ttctcgtcgc gctgcacgcg
     8941 gcgatgacgc cggcgccgcg ccacatcacg cagcgcgaca tcgacaacgc ggtgcgccat
     9001 tcgctcgaga cccatccgct tccgtcgcgc gcggcgcagg cctacgaggc ggtcgcgccc
     9061 gccatagtcc gggtgcaggg cgtgcgcaag gccgaggcgg tcgccgacgg cgcggcgccg
     9121 tccgaaggga gcacgggcag cggcgtcgtc gtccacgaga gcggcgtgat cctcacgagc
     9181 ctgcacgtga tcgagggtac cgacagcatc gcggtcgact tcgcgaccgg cgagcattcg
     9241 gaggccgaag tcatcgccgt gcagcccgag aacgatctcg cggtgctgcg ggcgcgcacg
     9301 ttgcccgacg atctggtgcc ggcgacgctg ggcgccgccg cggacctgaa gcccggcgac
     9361 gacgtgatcg cggtcggctt cccgttcggc atcggcccgt cggtgacctc gggcgtgatc
     9421 tcggggctga accgcgagta ccaggcgccc gacggcgggc gctcgctcag ccgcctcatc
     9481 cagttcgacg cggccgcgaa ccccggcagc tcgggcgggc cgctggtcac cgccaccggc
     9541 gaggtggtcg gcatcgtcac cggcattctc aatcccagcg atcagcgcgt gttcgccggc
     9601 atcgcgttcg cggtgccgat cgagaccgcg tcgtccgcgg tcggccttcc tcctttctga
     9661 ggtgatcatg tcgtcttccc tgctcgagcg catcctgtac gaggtcaaga aagtcgtcgt
     9721 cggccaggac catttcctcg agcgcgtgct ggtcgccatc ctcgcgcagg ggcacctcct
     9781 ggtcgagggc gtgccgggcc tcgccaagac gctcaccgtc aagacgctcg cgcgcaccat
     9841 ccgcggctcg ttccggcgca ttcagttcac ccccgacctc gtgccgtccg atctcgtcgg
     9901 cacacgcatc tacaaccagc ggacgggcga tttcgccacc tcgctcggcc cggtcttcgc
     9961 gaacctgctg ctcgccgacg agatcaaccg cgcgcccgcg aaggtgcaga gcgcgctgct
    10021 cgaggtgatg caggaatacc aggtgacgat cgccggcgag acgcaccatg tgcccgagcc
    10081 cttcctcgtc atggcgacgc agaacccgat cgagaccgag ggcacgtacc cgctgcccga
    10141 ggcgcaggtc gaccgcttca tgatgaaggt gctcgtcggc tatccgagcg aggaagagga
    10201 gttcgtgatc gtgaaccgcg tcaccgggcc gtcggtcgac gtcgcccccg tcgcgaccac
    10261 cgagcaactc gcggagctgc agcgcgagtg ccggcgcagc tacgtcgatc cggcgctcat
    10321 tcagtacgcg gtgcggctcg cgggcgcgac ccgcgatccg gagcgccacg gcatcccgga
    10381 cctcgcgcac tacatctcgt acggcgcgag cccgcgcgcg tcgatccacc tgatcgaggc
    10441 ggggcgcgcg ctcgccttcc ttcgcggacg cgactacctc ttgccgcagg acgtcaccga
    10501 cgtcacgccc gacgtcctgc gccaccgcgt cgtgctctcg tacgaggcgc tcgcgaacgg
    10561 cgtgacgccc gacgagatcg tgcagcgcgt cctcgagcac gtccgcgcgc ccgccaagcc
    10621 catggagcga catgtccagg ccgccgcagg ctgaggcggt cctgcgccgg ctcgagtgga
    10681 cggtcatccg tcggctcgac ggcctgctgc acggcgacta ccgcacgctg tttcgcggct
    10741 tcgggctcga cctcgccgac ctgcgcgagt accagtacca cgacgacgtg cggcgcatcg
    10801 actggaacgt caccgcgcgg ctcggcacgc cctatgtgcg cgagtaccac gaagaccgcg
    10861 aggtgaccgc ctggttcctc ctggatctct cgccgtcggt cgacttcggc tcgcgcaccc
    10921 gcaagcgctc ggtgtccgcc gagttcgtcg ccgtgctcgc gcgcctgctc acccgccacg
    10981 gcaattcggt gggcgcgatg ttctacggcg accggctcga cgcggtcatc ccaccgcgca
    11041 gcagccggcg ccacgtgctg cacctgctcg aggcgatgcg cgcgcggccg ccgctcgcgg
    11101 cgtcgcgccc gaccgacctg cgcgcgttcc tgcgctcggc gttcgggatg ctcggccggc
    11161 gctcggtcgt cttcgtcgtc tcggacttca tcagcgcgcc gggctgggcc gagccgctcg
    11221 cgctgctcgc gcagagccac gacgtgctcg cggtgcgact ccacgacgtg ctcgagagcg
    11281 agatgcccga cctcgggctg ctcgtcgtgc aggacgccga gaccggcgag cagttgttcg
    11341 tcgacacgca cgaccggggc ttccgccgcc gcttcgcgcg cgcggccgaa gcgcgcgaag
    11401 gcgcggtgcg cgccgcgttc gcgcaggcgg gcgtcgacgc gctcgagctc tcgaccgacg
    11461 gcgacctggt cgacgcggtg ctgcgcttcg ccgacctgcg caagcgccgc gtgcggcgcc
    11521 cggcgggcgc gccggcgccg ctgcgcgcgg gagtgacggc atgaccttcc tctggcccga
    11581 cctgctgtgg ctgctcgccg ccgtgccgct cctggtcgcg gcctacgtgc tcgcgctgcg
    11641 ccggcgcaag aaggcggcgc tgcgctacgc gagcctcgag gtcgtgaagg cggcgatggg
    11701 ccccggacag tcggtgcgcc ggcacgtgcc gcccgcgctg ttcctcgcgg cgctggtgct
    11761 gctgatcgtc gccgtcgcgc gcccggccgc ggtcgtgaag ctcccgagcg acgacaagac
    11821 gatcatcctc gcgatcgacg tctcgggcag catgcgcgcc gaggacgtac agcccaaccg
    11881 cctggcggcg gcgcaggctg ccgcgcgcca gttcgtcagc gaccagccct cgggcaccaa
    11941 ggtcgggctg gtcgcgttcg cggggaccgc cgcgctggtg cagccgccga cgcgagggcg
    12001 cg
//
LOCUS       DATDMW010000400         2146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1364670, whole genome shotgun sequence.
ACCESSION   DATDMW010000400 DATDMW010000000
VERSION     DATDMW010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364670"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VI319_16665"
     CDS             <1..425
                     /locus_tag="VI319_16665"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HEY6865527.1"
                     /translation="TAPLRSSGTLVYVAPGHLEKQTTKPAPESLVLDGERLAIGKIEG
                     GPKRVLNLPDYPVLWAFVESIRSTLAGDLGTLNRFYRVELSGRESQWRLVLVPVEPKM
                     REVVREIRIDGRRDWIDTISVFESGGDRSTMTITRDAS"
     gene            422..>2146
                     /locus_tag="VI319_16670"
     CDS             422..>2146
                     /locus_tag="VI319_16670"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY6865528.1"
                     /translation="MSRAGRSPVLAWLAGAVACVWVIAHTEFSTDLSAFLPRSPSAAQ
                     QVLVEQLREGVVSRLILVGIEGDAPETLAEASKRVTGSLRHDARFASVDNGDDAAIEA
                     DRDFLWRNRYLLSPGVTPERYSAAGLRASLQEDLQMLNSPAGMLMRRALPSDPGGEML
                     RLLSQLQGAARPSTRHGVWFSADGRRALLVAQTRAAGYDIDAQEHAVAAIRAAFEPAR
                     RGTSAKLLLAGPGVFSVYARERIKGDALRFSMIATALIGAMLLALYRSPRVLGLALLP
                     VASGALAGVAAVGLAYGSVHGVTLGFGVTLIGEGVDYAIYLLTRIAPGAPPRAAFERI
                     WPTLRLGVLTSICGFSAMLLSGFSGLAQLGLFSIAGLIVAAAVTRWVLPALIPDAFKG
                     PRIAALERGAMSLLAWAPKLRVPALAAVLVAGAVLVVHRGSAWSDDLADLGPVTAEEK
                     SLDGQLRRDLGAPDVRHLVVVHAADRDAALAACERIAPALERSVARGWLEGFESPATS
                     LPSRETQRARQSALPPAQDLRANLQKALQGLPFRTGLFEPFVAEAEAARVAPPLGRDA
                     LEGTHFALK"
BASE COUNT          257 a          787 c          787 g          315 t
ORIGIN      
        1 tgaccgcgcc gctgcgctcg tcggggacgc tcgtctacgt cgcgcccggc cacctcgaga
       61 agcagacgac gaagcccgcc cccgaatcgc tcgtgctcga cggcgagcgg ctcgcgatcg
      121 ggaagatcga gggcgggccg aagcgcgtgc tgaacctccc cgactacccg gtgctgtggg
      181 cgttcgtcga gagcatccgc tcgaccctcg ccggggatct cggcacgctg aaccggttct
      241 accgcgtcga gctgagcggc cgcgaatcgc aatggcgcct cgtgctcgtt ccggtcgagc
      301 cgaagatgcg cgaggtcgtg cgcgagatcc gcatcgacgg ccggcgggac tggatcgaca
      361 cgatctcggt cttcgagtcg ggcggagacc gctcgaccat gaccatcacc cgggacgcct
      421 cgtgagccgc gccgggcgct cgccggtgct cgcctggctc gccggtgccg tcgcgtgcgt
      481 gtgggtcatc gcgcacaccg agttcagcac cgacctctcg gccttcctgc cgcgctcgcc
      541 gtcggccgcg cagcaggtgc tggtcgagca gctgcgcgag ggcgtggtgt cgcggctgat
      601 cctcgtcggg atcgaaggcg acgcgccgga gacgctggcc gaggcgagca agcgcgtgac
      661 cggatcgctg cgccatgacg cgcgcttcgc ctccgtcgac aacggcgacg acgccgcgat
      721 cgaggcggac cgcgatttcc tctggcgcaa ccgctacctg ctcagtcccg gcgtgacgcc
      781 cgagcgctac agcgccgcgg gcctgcgagc gagcctgcag gaagacctgc aaatgctcaa
      841 ttcgccggcc ggaatgctga tgcgccgcgc gctgccctcc gacccggggg gcgagatgct
      901 gcggctgctc tcccagctgc agggcgccgc ccgtccgtcg acgcgccacg gagtctggtt
      961 ctcggccgac ggcaggcgcg cgctgctggt cgcgcagacg cgcgcggcgg gctacgatat
     1021 cgacgcgcag gagcacgccg tggcggcgat ccgcgcagca ttcgagccgg cgcggcgcgg
     1081 caccagcgca aagctcctgc tcgcggggcc cggcgtattc tcggtctacg cccgcgagcg
     1141 catcaaggga gacgcgctgc gcttctcgat gatcgcgacg gcgctgatcg gcgccatgct
     1201 gctcgcgctg taccgatcgc cgcgcgtgct cgggctcgcc ctcttgccgg tcgcgagcgg
     1261 ggcgctcgcg ggcgtcgcgg cggtgggtct tgcctacggc tcggtgcacg gcgtcacgct
     1321 cggcttcggc gtcacgctga tcggggaagg tgtggactat gcgatctacc tgctcacgcg
     1381 catcgcaccc ggggcgccac cgcgcgcggc gttcgagcgc atctggccga cgctgcggct
     1441 cggcgtgctg acctcgatct gcggcttctc ggccatgctg ctctcgggct tcagcggtct
     1501 cgcacagctc gggctgttct cgatcgccgg gctcatcgtc gcggcggcgg tgacgcgctg
     1561 ggtgctgccg gcgctgatcc ccgacgcgtt caagggaccg cgcatcgcgg cgctcgagcg
     1621 aggcgcgatg tcgctgctcg cgtgggcccc gaagctgcgc gtcccggccc tcgccgccgt
     1681 gctcgtcgcc ggcgcggtgc tggtcgtgca ccgcggttcg gcctggagcg acgacctcgc
     1741 cgatctcggg ccggtcacgg cggaggagaa gagcctcgac ggacagctgc gccgcgacct
     1801 gggcgcgccc gacgtgcgcc atctcgtggt ggtccacgcc gccgaccgcg acgcggcgct
     1861 cgcggcgtgc gagcgcatcg cgcccgcgct cgagcgctcc gtggcccgtg gctggctcga
     1921 agggttcgag tcgcccgcca cgtcgcttcc gagccgcgag acgcagcgcg cgcggcagtc
     1981 cgcgctgccg ccggcgcagg acctgcgcgc gaacctgcag aaggcgctcc aggggctgcc
     2041 gttccgcacc gggctgttcg agcccttcgt cgcggaggcc gaggcggcgc gcgtcgcgcc
     2101 gccgctcggc cgcgacgcgc tcgaagggac gcacttcgcg ctcaag
//
LOCUS       DATDMW010000401        12577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_741664, whole genome shotgun sequence.
ACCESSION   DATDMW010000401 DATDMW010000000
VERSION     DATDMW010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12577
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741664"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="VI319_16675"
     CDS             complement(<1..1944)
                     /locus_tag="VI319_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6865529.1"
                     /translation="MNPSATFIARPVATTLLTIGIALAGAAAFTLLPVAPLPRVDYPT
                     ISVSANLPGASPETMAATVATPLERALGRIAGVNEITSGSSLGSSRITLQFDLDRDID
                     GAARDVQAAIMASRSLLPTGLPNNPTYRKVNPADAPILLLSLTSDTMSRGQMYDAAST
                     ILAQKLSQVAGIGQVTVGGSSLPAVRVELNPLALHKYGIGLEDVRNAIVATNANRPKG
                     SIEDGDRHWQIGANDQAKVAADYVPLVITYRDGAAVRLSDVAQVLDSEQDVRNMGSAN
                     GRPAVVVVLNRQPNANIIETVDRVKALLPQLRASIPSAIDMKIAMDRTTTIRASLRDV
                     ERALAMAVGLVILVVFLFLRNARATAIPAVAVPISLLGTLGAMYLLGYSLDNLSLMAL
                     TIATGFVVDDAIVVLENVSRHIEEGVPPMRAALLGAREVGFTVVSMSFSLIAVFIPIL
                     LMGGIIGRLFREFAVVLSVAILVSLVLSLTTTPMMCARLLRPRPADAAREPGRVSRAA
                     ARAYAAAIAGYGRSLAWALRHGPLMMGVLAATIVLNIALYYTIPKGFFPRQDTGRIVG
                     SIQADQSISFQAMQQKLAGFVDIVKADPAVENVVAFTGGGQRNRGFMFVVLKSLSDRK
                     VSAEQVIARLRVKLAREPGASLFL"
     gene            complement(1941..5138)
                     /locus_tag="VI319_16680"
     CDS             complement(1941..5138)
                     /locus_tag="VI319_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6865530.1"
                     /translation="MNPSRLFILRPVATSLLMVAVLLSGIIAYRVLPVSSLPEVDYPT
                     IQVVTLYPGASPDVTTSSITAPLERQFGQMPGLNKMSSTSSGGASVITLQFSLELSLD
                     VAEQEVQAAINASSNLLPSDLPVPPIYNKVNPADAPIMTLAVTSATLPLPALQNLVDT
                     RLAQKISQLPGVGLVSLSGGQRPAVRIQANPAALAAYGLSLEDLRTAIANANVNQAKG
                     SFDGPARASTIDANDQLRSADEYRNLIVAYRGGAPIRLSDVAQAVDGAENVRLAAWAN
                     EAPGIIVNIQRQPGANVIEVVDRVNALLPQLRETLPGSVDVAQLTDRTVTIRASVHDV
                     QFELMLAVALVVMVIFLFLRSPSATAIPSVAVPLSLVGTFGVMYLAGFSINNLTLMAL
                     TIATGFVVDDAIVMIENISRYIEEGDSPLEAALKGSEQIGFTIISLTFSLIAVLIPLL
                     FMGDVVGRLFREFAITLAVSILISAVVSLTLTPMMCAKLLKRTPEREQGRFYRASQHA
                     FDRMIARYGRMLNWVLDRQGATLLVFFATLALSVLLYLLVPKGFFPVQDTGVIQGITE
                     APQSISFAAMGERQQALAREVLKDPAVESLSSFIGVDGINTTLNSGRMQINLKPLGQR
                     GPEPRINATDVIRRLQQRVAGVEGITLYMQPVQDLTIEDHVSRTQYQMTVEDANPAEL
                     SAWVPKLVERLRRIPDLAGVASDLQDQGLQAYVEIDRATAGRLGVTPAAIDAALYNAF
                     GQRLVSTIFTQSNQYRVVLEVKPEFNSGGPLALGDIYVKASPGAAAAGAAQVPGAQTT
                     NTAAAGSASSGTAGSQVRLSSVARIVERAAPLAINHIGQFPSATISFDVAPGSSLGRA
                     VDAIRAAQAEIGLPASVQTRFQGAALAFQASLDNTLLLIVAAIVTMYIVLGVLYESYI
                     HPVTILSTLPSAGVGALLALMVSRTDLGIIAVIGIILLIGIVKKNAIMMIDFALDAER
                     KEGKAPREAIFQACLLRFRPILMTTMAALLSALPLMLGRGVGSELRHPLGITMVGGLL
                     VSQLLTLFTTPVIYLAFDRLSLRFRGKRLAAGTPP"
     gene            complement(5135..6451)
                     /locus_tag="VI319_16685"
     CDS             complement(5135..6451)
                     /locus_tag="VI319_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HEY6865531.1"
                     /translation="MTDMSPNLLPERVVRNDARARRRRWIIASALVLAALIAALVAWL
                     VLRPSPAQGPAGDVAQRGARGAAGGGAARAMPVVAAAARTADVGIYLSGLGSVTPIAT
                     VTVKSRVDGQLMKVSFREGQLVRAGDVLAEIDPRPYQALVAQAEGTLARDAALLKNAE
                     VDLERYRTLFKQDSVAKQQLDTQEALVRQYEGTVKVDDAAIQTARLNLSYCRITAPIS
                     GRLGLRQVDPGNIVHASDSSGVVVITQLQPIAVLFTIPEDNVPVVMKRLQSGDKLPVD
                     AWDRADKTRLASGTLTTVDNQIDPSTGTVKLKAQFPNQDYGLFPNQFVNARMLVDVHR
                     GATVVPAAAVQRGSRGTFVYVVNPDNTVSVRAVRVGASQGDDVAIDDGVTPGEVVVTD
                     GADKLREGASVDVAGRAAPPAGDAPRKGGGEGREGRKGGGGRKGGG"
     gene            complement(6577..7707)
                     /locus_tag="VI319_16690"
     CDS             complement(6577..7707)
                     /locus_tag="VI319_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY6865532.1"
                     /translation="MRRGPVCALLLAAAIAVPAHAHTPSDSYLALRIEDARVAGQWDI
                     ALRDLDFAIGLDSNGDGDITWGELRARHADIAAYALARLKLRSGGADCPLQAGEHLVD
                     DHSDGAYDVMRFSATCPGKIDKLDISYSLFADLDPQHRGLLRIEQGAATRTAIFGPDR
                     PTQTIDVATISAWTQFVDYGREGVWHIWIGFDHILFLLSLLLPAVLTREPLGWRPVQA
                     FKPAFMDVFKIVTSFTVAHSITLSLATLGVVSLPSRVAESAIALSVVLAALNNVFPLV
                     QGRRWVVAFAFGLIHGFGFASVLKDLGLPQSALLLALVGFNLGVEVGQLAIVSVFLPA
                     AYALRRQWVYRGLVFVAGSWVIVAVAGTWLIERVFDLKLISR"
     gene            complement(7704..8792)
                     /locus_tag="VI319_16695"
     CDS             complement(7704..8792)
                     /locus_tag="VI319_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865533.1"
                     /translation="MLERLPVRPGDPVARELRQLRARLAEDPRDAGNALALSERYFEL
                     ANAEGDPRYVGYAQAALGPWWDLPDPPQELRVMRAMLKQYSHDFAGAMTDLEAATRAD
                     PEDAQAWSWRGAIHMVQADYAAARADCEAQRRIESELGYVGCIAYLDGVTGHAAAAYQ
                     ALSQALARARGASPEEKTWVQTRLAELGQRMGDDALAERHFKAAFEQKVTDQFLLAAW
                     ADFLLDHGRAAEVVPLLRDWTRSDILLLRLALAEQALKLPEAKEHVAALKARFDAAAL
                     RGDKLHQQEEARFNLYLVGDKAKALALAQENWKLQREPRDARILLESALAMKDPQGAR
                     EALDWLERSGYEDPLLRDEAAKLKGVKP"
     gene            complement(8902..9951)
                     /locus_tag="VI319_16700"
     CDS             complement(8902..9951)
                     /locus_tag="VI319_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY6865534.1"
                     /translation="MAPGRAGVGAALLGAFLFGASTPVAKLLVGEIDPLLLAGLLYAG
                     SGIGLTLWLLARRVRPGASRIALPRGDVPWLAGAVIAGGVAAPVLLMLGLASTEASTA
                     ALLLNLEGVFTALIAWFAFRENFDRRVLAGMVLIVAGGLLLAWRPGGFTLAWPAVAIA
                     AACLLWALDNNLTRKVSTSDAVVIAGVKGLVAGAVNVSLALALGSAAPAAKLWLAASA
                     VGFAGYGVSLALFVVALRQLGAARTAAYFSTAPFIGAALAFAALGESQTPLFWIAALL
                     MAAGVALHLAERHAHGHTHQRLRHEHAHLHDEHHRHEHAAGWDGREPHTHEHEHAPLA
                     HSHPHYPDIHHRHSH"
     gene            complement(9951..10361)
                     /locus_tag="VI319_16705"
     CDS             complement(9951..10361)
                     /locus_tag="VI319_16705"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY6865535.1"
                     /translation="MGLFSGKRPDNLGAIRGRLAPCRSTPNCVSSQADLGDPTHYIEP
                     LRFVGEPAHAWAALQVVVSGMERARIVGRENAYLYAEFTSRLMGYVDDVEFLLDAPAR
                     LIHVRSASRLGIRDFGVNRERIETIRGRLMAAGV"
     gene            complement(10383..11177)
                     /locus_tag="VI319_16710"
     CDS             complement(10383..11177)
                     /locus_tag="VI319_16710"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6865536.1"
                     /translation="MFWSLARIGLAVVVGVPMFVYLLQEKLLFFPQPLGKPINVPNVE
                     EVKITTPDGVTLQGWLVKGHHAQPAPLVIYFGGNAEEVSWLASVADQFGGWSLLLVNY
                     RGYGGSEGRPGERELFADALAIHDWAIRRADILPERVIAMGRSLGSGVAVHLAASRNL
                     RGVILVTPYDSITEIAKRHYPYLPVGMLIRHPFDSISRVDKIDTPLLCLAAGEDLVVP
                     PEHARRLYDAWRGPKTWRELAGAGHDSISGEPEYWEAIGTFLKDRR"
     gene            complement(11191..12504)
                     /locus_tag="VI319_16715"
     CDS             complement(11191..12504)
                     /locus_tag="VI319_16715"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6865537.1"
                     /translation="MASPHHIVIVGGGAGGLELATRLGDRLGRRGHAAVTLVDRSPTH
                     LWKPLLHEVAAGSLDRHANELDYLAQARWHHFTFAQGALEGLDRWKRELAVAPVLDED
                     GAEVIPRRTIRYDTLVIAIGSVSNSFNIPGVAEHALLLDSPDQADRLHRRVVNACLQA
                     NYRAGDQKDGRLDMVIVGGGATGVELAAELRNTTRVLAGYGLEHIDPDRYVRLTLVNA
                     DPRILAVLPERISEATTDVLKSLGVDVRNAAQVTEVTADAVRTKGGDSFRADITVWAA
                     GIKCAEVLKNLADLETNRLNQLVVKPTLQTTRDPDVFAFGDCASCPWPGHPAPVPPRA
                     QAAHQQASHLVRMMERRLRGEALLPFRYRDFGSLVSLGEYSTVGTLMGFVSGKSLRVE
                     GWFARLMYISLYKLHLLALHGFVGVALDTLARALKRSTEPQVKLH"
BASE COUNT         1893 a         4393 c         4504 g         1787 t
ORIGIN      
        1 gagaaacagg ctcgcgccgg gctcgcgcgc gagcttcacg cgcaggcgcg cgatgacctg
       61 ctcggccgag actttgcggt cgctcagcga cttcagcacc acgaacatga agcctcggtt
      121 gcgctgcccg ccgcccgtga acgcgaccac gttctccacc gccggatcgg ccttgacgat
      181 gtcgacgaag cccgcgagct tctgctgcat cgcctggaac gagatgctct gatcggcctg
      241 gatgctgccg acgatgcgac cggtgtcctg gcgcgggaag aagcccttcg gaatcgtgta
      301 gtacagcgcg atgttgagaa cgatcgtcgc ggcgagcacc cccatcatca gcggcccgtg
      361 gcgcagcgcc cacgcgagcg agcgcccgta gcccgcgatc gccgccgcgt acgcgcgcgc
      421 cgccgcgcgc gaaacgcgcc ccggctcgcg cgccgcatcc gccggccgcg ggcgcagcag
      481 ccgcgcgcac atcatcggcg tcgtcgtcag cgagagcacg agcgatacca ggatcgcgac
      541 cgagagcacg accgcgaact cccggaagag gcgcccgatg atcccgccca tcagcaggat
      601 gggaatgaac accgcgatca gggagaagct catcgacacg accgtgaagc cgacctcgcg
      661 cgcgccgagc agggccgcgc gcatcggcgg gacgccctcc tcgatgtggc gcgacacgtt
      721 ctcgaggacg acgatcgcgt cgtcgacgac gaagccggtc gcgatggtga gcgccatgag
      781 cgacaggttg tccaggctgt agccgagcag gtacatcgcg ccgagcgtcc cgaggagcga
      841 gatcggcacc gcgaccgcgg ggatcgcggt ggcgcgcgcg ttgcgcagga acaggaacac
      901 caccaggatc accaggccga ccgccatcgc gagcgcgcgc tccacgtcgc gcagcgacgc
      961 gcggatcgtc gtcgtgcggt ccatggcgat cttcatgtcg atcgcgctcg ggatcgacgc
     1021 gcgcagctgc ggcagcagcg ccttcacccg gtccacggtc tcgatgatgt tcgcgttcgg
     1081 ctggcggttc aggacgacga cgaccgccgg gcggccgttg gccgagccca tgttgcgcac
     1141 gtcctgctcg gaatcgagca cctgggccac gtccgagagg cgcaccgcgg cgccgtcgcg
     1201 gtaggtgatc acgagcggga cgtagtcggc ggcgaccttg gcttggtcgt tggcgccgat
     1261 ctgccagtgg cggtcgccgt cctcgatcga gcctttcggg cggttggcgt tggtcgcgac
     1321 gatggcgttg cgcacgtcct cgagtccgat cccgtacttg tgcagtgcca gcgggttgag
     1381 ctcgacgcgc acggcgggca gcgagctgcc gcccacggtc acctggccga tcccggcgac
     1441 ctgcgagagc ttctgcgcga ggatggtcga ggccgcgtcg tacatctggc cgcgcgacat
     1501 cgtgtccgag gtcagcgaga gcagcaggat gggcgcgtcg gccgggttga ccttgcggta
     1561 ggtcgggttg ttcggcagcc ccgtcggcag gaggctgcgc gacgccatga ttgccgcctg
     1621 cacgtcgcgc gccgcgccgt cgatgtcccg gtcgaggtcg aactgcagcg tgatccgcga
     1681 cgacccgagc gaggagcccg aggtgatctc gttcacgccg gcgatgcggc cgagcgcgcg
     1741 ctcgagcggc gtcgccaccg tcgccgccat ggtctccggg ctcgcgccgg gcaggttcgc
     1801 cgacaccgag atcgtgggat agtcgacgcg cggcagcggc gcgacgggca gcagcgtgaa
     1861 cgcggccgcg ccggcgagcg cgatgccgat cgtgagcagc gtggtcgcga ccgggcgcgc
     1921 gatgaacgtg gccgacgggt tcacggcggc gtcccggcgg cgaggcgctt gccgcggaag
     1981 cgcagcgaca ggcggtcgaa cgcgaggtag atcaccggcg tggtgaacag cgtcagcagc
     2041 tggctcacca gcagcccgcc caccatggtg atgccgagcg ggtgccgcag ctcggagccc
     2101 acgccccggc cgagcatcag cggcagcgcc gagagcagcg ccgccatcgt cgtcatcagg
     2161 atcggccgga agcgcagcag gcaggcctgg aagatcgcct cgcgcggcgc cttgccttcc
     2221 ttgcgctcgg cgtcgagcgc gaagtcgatc atcatgatcg cgttcttctt gacgatgccg
     2281 atcagcagga tgatgccgat cacggcgatg atgccgaggt cggtgcgcga caccatcagc
     2341 gcgagcagcg cgccgacgcc cgccgacggc agcgtcgaca ggatcgtcac ggggtggatg
     2401 tagctctcgt acaggacgcc cagcacgatg tacatcgtca cgatcgccgc gacgatcagc
     2461 agcagcgtgt tgtcgagcga cgcctggaac gcgagcgccg cgccctggaa gcgcgtctgc
     2521 acgctcgcgg ggagcccgat ctcggcctgg gccgcgcgga tcgcgtcgac cgcgcgcccg
     2581 agcgacgacc ccggcgcgac gtcgaacgag atcgtcgccg agggaaactg cccgatgtgg
     2641 ttgatcgcga gcggcgcggc gcgctcgacg atgcgcgcca ccgaggacag gcgcacctgc
     2701 gagccggcgg tgcccgacga cgccgagccc gccgccgccg tgttcgtcgt ctgcgcgccc
     2761 gggacctgcg ccgcccccgc ggccgccgcg ccggggctcg ccttcacgta gatgtcgccg
     2821 agcgcgagcg gcccgccgga gttgaactcg ggcttcacct cgagcacgac gcggtactgg
     2881 ttcgactgcg tgaagatggt cgacaccagt cgctggccga aagcgttgta gagcgccgcg
     2941 tcgatcgccg ccggcgtcac gcccaggcgc cccgcggtgg cgcggtcgat ctcgacgtag
     3001 gcttgcagcc cctggtcctg caggtcgctc gcgacgccgg cgagatcggg gatgcggcgc
     3061 agccgctcga ccagcttcgg cacccaggcc gagagctcgg ccgggttcgc gtcctcgacc
     3121 gtcatctggt actgcgtgcg gctgacatgg tcctcgatcg tgaggtcctg cacgggctgc
     3181 atgtacagcg tgatgccctc gacgcccgcg accctctgct gcagcctgcg gatcacgtcg
     3241 gtcgcgttga tgcgcggctc gggcccgcgc tgccccagcg gcttcaggtt gatctgcatg
     3301 cggccgctgt tcagcgtggt attgatgccg tcgacgccga tgaacgagga caggctctcg
     3361 accgcggggt ccttcagcac ctcgcgcgcg agcgcctgct ggcgctcgcc catggccgcg
     3421 aacgagatcg actgcggggc ttcggtgatg ccctggatca cgcccgtgtc ctgcaccggg
     3481 aaaaagccct tcggcaccag caggtagagc agcaccgaga gcgcgagcgt ggcgaagaaa
     3541 acgagcagcg tcgccccctg gcggtcgagc acccagttca gcatccggcc gtagcgggcg
     3601 atcatgcggt cgaacgcgtg ctggctcgcg cggtagaagc gcccctgctc gcgctccggc
     3661 gtgcgcttca gcagcttcgc gcacatcatc ggcgtcagcg tcagcgacac cacggccgag
     3721 atcaggatcg agacggcgag ggtgatcgcg aactcgcgga acaggcgccc gaccacgtcg
     3781 cccatgaaca gcagcgggat cagcaccgcg atcagcgaga aggtcagcga gatgatcgta
     3841 aatccgattt gctccgagcc cttcagcgcc gcttccagcg gcgagtcgcc ctcctcgatg
     3901 tagcgcgaga tgttctcgat catgacgatg gcgtcgtcga ccacgaagcc cgtggcgatg
     3961 gtcaacgcca tcagcgtcag gttgttgatg ctgaagccgg cgaggtacat cacgccgaag
     4021 gtcccgacca gggacagcgg caccgcgacg ctgggaatgg cggtggcgga cgggcttcgc
     4081 aggaacagga agatcaccat cacgaccagc gccacggcca gcatgagctc gaactgcacg
     4141 tcgtgcaccg aggcgcggat cgtcacggtg cggtcggtga gctgcgcgac gtcgaccgac
     4201 cccggcagcg tctcccgcag ctgcggcagc agcgcgttca cgcggtccac cacctcgatg
     4261 acgttggcgc ccggctggcg ctggatgttg acgatgatgc ccggcgcctc gttcgcccac
     4321 gcggcgaggc gcacgttctc ggcgccgtcg accgcctgcg ccacgtcgga gagccggatc
     4381 ggggcgccgc cgcggtaggc gacgatcagg ttgcggtact cgtcggccga gcgcagctgg
     4441 tcgttcgcgt cgatcgtcga cgcccgcgcc gggccgtcga agctgccctt cgcctggttg
     4501 acgttggcgt tcgcgatcgc cgtgcgcagg tcctcgagcg acagcccgta ggccgcgagc
     4561 gcggccgggt tcgcctggat gcgcaccgcc gggcgctgcc cgcccgagag gctgacgagg
     4621 cccacgcccg ggagctgcga gatcttctgc gcgagccgcg tgtcgaccag gttctgcagc
     4681 gccggcaacg gcagcgtcgc cgaggtcacc gcgagcgtca tgatgggcgc atcggccggg
     4741 ttgaccttgt tgtagatcgg cggcaccggc aggtccgaag gcagcaggtt cgacgacgcg
     4801 ttgatcgccg cctgcacctc ctgctcggcg acgtcgagcg aaagctcgag gctgaactgc
     4861 agcgtgatga ccgacgcgcc gcccgagctc gtcgacgaca tcttgttcag ccccggcatt
     4921 tggccgaact ggcgctcgag cggcgccgtg atcgaggacg tggtcacgtc ggggcttgcg
     4981 ccgggataga gcgtgacgac ctggatcgtg gggtagtcga cctcgggcag cgacgacacc
     5041 ggcagcaccc ggtaggcgat gatccccgag agcagcaccg cgaccatcaa aagcgaggtc
     5101 gccaccggcc gcaggatgaa caggcgcgac gggttcatcc cccgcctttg cgacccccgc
     5161 cgccttttcg tccctcccgc ccctcgccgc cgcctttgcg gggtgcgtcg cccgccggtg
     5221 gcgccgcgcg gccggcgacg tccacgctcg ccccttcgcg cagcttgtcg gcgccgtcgg
     5281 tgaccacgac ctcgcccggc gtgacgccgt cgtcgatcgc gacgtcgtcg ccctgcgagg
     5341 ccccgacgcg cacggcgcgc acgctcaccg tgttgtcggg gttgacgacg tagacgaagg
     5401 tgccgcgcga tccgcgctgc accgccgcgg ccgggaccac ggtggcgccg cggtgcacgt
     5461 cgaccagcat gcgcgcgttg acgaactgat tcgggaacag cccgtaatcc tggttcggga
     5521 actgcgcctt cagcttgacc gtgccggtgg acgggtcgat ctggttgtcg acggtcgtca
     5581 gcgtgccgct cgcgagccgg gtcttgtcgg ctcgatccca ggcgtcgacg ggaagcttgt
     5641 cgcccgactg cagccgcttc atcaccacgg ggacgttgtc ctcggggatc gtgaacagca
     5701 cggcgatcgg ctgcagctgc gtgatcacca cgacgccgct cgagtcgctc gcgtgcacga
     5761 tgttgccggg gtcgacctgg cgcagcccca gacggccgct gatcggcgcg gtgatgcggc
     5821 agtacgacag gttcaggcgc gccgtctgga tcgcggcatc gtcgaccttc accgtgccct
     5881 cgtactgccg cacgagcgcc tcctgcgtgt cgagctgctg cttcgcgacc gagtcctgct
     5941 tgaacagcgt gcgatagcgc tcgaggtcga cctcggcgtt cttgagcaga gccgcgtcgc
     6001 gcgcgagcgt gccttcggcc tgcgcgacca gcgcctggta cgggcgcggg tcgatctcgg
     6061 cgagcacgtc gcccgcgcgc acgagctggc cctcgcggaa cgagaccttc atcagctgac
     6121 cgtcgacgcg gctcttcacc gtcaccgtcg cgatcggagt gaccgagccc aggcccgaga
     6181 ggtagatgcc gacgtccgcg gtgcgagcgg ccgccgcgac gaccggcatg gcgcgcgcgg
     6241 cgccgcctcc ggccgcgcct ctggcgccgc gctgcgcgac gtcgccggcc ggaccctgcg
     6301 cgggagacgg acgcaggacc agccacgcca cgagcgcggc gatcagcgcg gcgagcacga
     6361 gcgcggacgc gatgatccac cgccggcgcc gcgcgcgcgc gtcgttacgg accacccgct
     6421 ccggcagcag gttcggagac atgtcggtca tgagggtgcc ctacgcatgc agcttagggc
     6481 ttttcccggc ggagcgccgt ctctgccatg taaagtattg ttgccattgg gtaacggacc
     6541 gtagacccgg ccgaaggttg ccgcggtcgg tcgtggtcag cgcgatatca gcttcaggtc
     6601 gaacacgcgc tcgatcagcc acgtcccggc gaccgcgacg atgacccacg atcccgccac
     6661 gaacacgagg ccgcggtaga cccattgccg ccgcagcgcg tacgccgcgg gcaggaacac
     6721 gctcacgatc gcgagctgac cgacctcgac gccgaggttg aatccgacga gcgcgagcag
     6781 aagggcgctc tgcggcagtc cgaggtcctt cagcacgctc gcgaagccga agccgtggat
     6841 caggccgaag gcgaacgcga ccacccagcg gcggccctgc accagcggga acacgttgtt
     6901 cagcgccgcc agcacgaccg acagcgcgat cgcggactcc gccacccgcg acgggagcga
     6961 cacgacgccc agggtcgcga gcgacagcgt gatcgagtgc gcgacggtga acgacgtgac
     7021 gatcttgaac acgtccatga acgccggctt gaaggcctgg accggccgcc agccgagcgg
     7081 ctcgcgcgtc agcaccgcgg gcagcagcag cgacagcagg aacaggatgt ggtcgaagcc
     7141 gatccagatg tgccagacgc cctcgcgccc gtagtcgacg aactgcgtcc aggccgagat
     7201 ggtcgcgacg tcgatggtct gcgtcgggcg gtcgggcccg aagatcgcgg tgcgcgtcgc
     7261 cgcgccctgc tcgatccgca gcagcccgcg gtgctgcggg tcgagatcgg cgaacaggct
     7321 gtaggagata tcgagcttgt cgatctttcc ggggcaggtc gccgagaaac gcatgacgtc
     7381 gtaggcgccg tcgctgtggt cgtcgaccag gtgctcgccc gcctgcagcg ggcaatcggc
     7441 gccgcccgag cggagcttca gccgcgcgag cgcgtacgcc gcgatgtcgg catggcgcgc
     7501 gcgcagctcg ccccaggtga tgtcgccgtc gccgttgcta tcgagaccga tggcgaagtc
     7561 gaggtcgcgc agcgcgatgt cccactgccc ggcgacgcgc gcgtcctcga tgcgcagcgc
     7621 gaggtagctg tcgctcggcg tgtgcgcgtg cgcgggcacg gcgatcgccg cggcgaggag
     7681 aagcgcgcaa accggcccgc gcctcacggc ttcaccccct tcagcttcgc cgcctcgtcg
     7741 cgcagcaagg ggtcctcgta gcccgaccgc tcgagccagt cgagcgcttc tcgcgcgcct
     7801 tgcgggtcct tcatcgcgag cgccgactcg agcaggatgc gcgcgtcgcg cggctcccgc
     7861 tgcagcttcc agttctcctg cgcgagggcg agggccttgg ccttgtcccc cacgagatac
     7921 agattgaacc gagcttcctc ttgctgatgg agcttgtccc cgcgcagcgc ggccgcgtcg
     7981 aaccgggctt tcagcgccgc gacgtgctcc ttcgcctcgg gcagcttcag cgcctgctcg
     8041 gcgagggcga gccgcagcag caggatgtcc gagcgcgtcc agtcgcgcag cagcggcacc
     8101 acctcggccg cgcggccgtg gtcgagcagg aagtcggccc aggcggcgag caggaactgg
     8161 tcggtgacct tctgctcgaa cgccgccttg aaatggcgct cggcgagcgc gtcgtcgccc
     8221 atgcgctggc cgagctccgc gagccgcgtc tgcacccagg tcttctcctc gggcgatgcg
     8281 ccgcgagcgc gcgcgagcgc ctgcgacagc gcctggtacg cggctgccgc gtggcccgtc
     8341 acgccgtcga ggtaggcgat gcagccgacg taaccgagct cgctctcgat gcggcgctgg
     8401 gcctcgcagt cggcgcgcgc cgccgcgtag tcggcctgca ccatgtggat ggccccgcgc
     8461 caggaccagg cctgcgcgtc ctcgggatcg gcgcgcgtcg ccgcctccag gtcggtcatc
     8521 gcgccggcga agtcgtggct gtactgcttc agcatcgcac gcatgacgcg cagctcctgc
     8581 ggcggatcgg ggaggtccca ccacgggccg agcgccgcct gcgcgtagcc gacgtagcgc
     8641 ggatcgccct cggcattcgc gagctcgaag tagcgctcgg agagcgcgag cgcgttgcct
     8701 gcgtcgcgcg gatcctcggc gagcctggcg cgcagctggc gcaactcgcg cgcgaccggg
     8761 tcgcccggac gcacgggcag gcgctcgagc acgaccgagt cgtccttggg gacatggggc
     8821 gcggcggccg acggcggcgc gagcgaggcg aggaggatga gcgcgatcgc ccgccttttc
     8881 tccgctcttg ttgttcttgt tctagtggga gtggcgatga tggatgtccg ggtagtgggg
     8941 atggctgtgc gcgagcggcg cgtgctcgtg ctcgtgcgtg tgcggctctc gtccgtccca
     9001 gccggcggcg tgctcgtgcc ggtggtgctc gtcgtgcagg tgcgcgtgct cgtggcgcag
     9061 gcgctgatgg gtatgaccgt gcgcgtgccg ctcggcgaga tgcagcgcga cgccggccgc
     9121 catgaggagc gcggcgatcc agaacagcgg cgtctgagac tcgcccagcg cagcgaaggc
     9181 gagcgccgcc ccgatgaatg gcgcggtcga gaagtacgcc gccgtgcgcg ccgcgccgag
     9241 ctggcgcagc gccacgacga agagcgcgag gctcacgccg tagcccgcga atccgaccgc
     9301 ggaggcggcg agccagagct tggcggcggg cgcggcgctc cccagcgcca gcgcgagcga
     9361 gacgttgact gcgccggcga ccagtccctt gacgccggcg atgacgaccg cgtcgctcgt
     9421 cgacaccttg cgcgtcaggt tgttgtccag cgcccacagc aggcacgcgg ccgcgatggc
     9481 gaccgcgggc cacgcgagcg tgaagccgcc gggacgccag gcgagcagca gcccgccggc
     9541 gacgatcagg accatgcccg ccagcacgcg ccggtcgaag ttctcacgga aggcgaacca
     9601 cgcgatcagc gcggtgaaga cgccctcgag attgagtagc agcgcggccg tcgacgcctc
     9661 cgtcgaggcg aggcccagca tgagcagcac gggcgccgcg acgccgccgg caatcacggc
     9721 gcccgcgagc cacggaacgt cgccgcgcgg gagcgcgatc cgggacgcgc ccgggcgcac
     9781 acggcgcgcg agcagccaga gcgtcagccc gatccccgag ccggcgtaga gcaggcccgc
     9841 gagcagcagc ggatcgattt ccccgaccag gagcttcgcg acgggcgtgc tggcgccgaa
     9901 gagaaaggcg ccgagcagcg cagcgccgac gcccgctcgg ccgggcgcca tcagacgccg
     9961 gcggccatga gccgcccgcg gatcgtctcg attcgctcgc ggttcacccc gaagtcgcgg
    10021 atcccgagcc gcgaagcgga gcgcacgtgg atcagccgcg ccggcgcgtc gagcaggaac
    10081 tcgacgtcgt cgacgtagcc catcagccgg ctggtgaact ccgcgtacag gtaggcgttc
    10141 tcgcgcccca cgatgcgcgc gcgctccatg cccgagacga ccacctgcag cgcggcccag
    10201 gcgtgcgcgg gctcgccgac gaagcgcagc ggctcgatgt agtgcgtggg gtcgccgagg
    10261 tcggcctggc tgctgacgca gttgggcgtc gacctgcagg gcgccagcct gccccggatg
    10321 gcgccgagat tgtccggccg ctttccgctg aagagtccca tcggccgagc attgtacgcg
    10381 cgctagcgcc ggtccttcag gaaggtcccg atcgcctccc agtactcggg ctcccccgag
    10441 atcgaatcgt ggccggcacc cgccagctcg cgccacgtct tcggcccgcg ccaggcgtcg
    10501 tacaagcgcc gcgcgtgctc gggcggcacg acgaggtcct cgcccgccgc gaggcacagg
    10561 agcggcgtgt cgatcttgtc cacgcgcgag atcgagtcga acggatggcg gatcagcatt
    10621 ccgaccggga ggtacggata gtggcgcttc gcgatctcgg tgatgctgtc gtaaggcgtc
    10681 accaggatca cgcccctcag gttacggctg gcggcgaggt gcaccgcgac gccgctcccg
    10741 aggctgcgcc ccatggcgat cacgcgctcg ggcaggatgt ccgcgcgccg gatcgcccag
    10801 tcgtggatcg cgagcgcgtc ggcgaacagc tcgcgctcgc cgggcctgcc ttcgctgccg
    10861 ccgtagccgc ggtaattgac gagcagcagc gaccacccgc cgaactggtc cgcgaccgag
    10921 gccagccacg acacttcctc ggcattgccg ccgaagtaga tcacgagcgg cgcgggctgc
    10981 gcatggtggc ctttcaccag ccatccttgc agcgtgacgc cgtcgggcgt cgtgatcttg
    11041 acctcctcga cgttggggac gttgatcggc ttgccgagcg gctgcgggaa gaacagcagc
    11101 ttctcctgca gcaggtacac gaacatcggc acgccgacga cgacggcgag cccgatgcgg
    11161 gcgagggacc agaacatttt catccgttct tcagtgcagc ttgacctgcg gctcggtgct
    11221 gcgcttgagc gcccgcgcga gcgtgtcgag cgcgaccccg acgaagccgt gcagggcgag
    11281 caggtgcagc ttgtagagcg aaatgtacat cagccgggcg aaccagccct cgacgcgcag
    11341 gctcttgccc gagacgaagc ccatcagcgt gccgaccgtg gaatattccc cgagcgagac
    11401 cagcgagccg aagtcgcggt agcggaaagg caggagcgcc tccccgcgca ggcgccgctc
    11461 catcatgcgc accaggtgcg aggcctgctg gtgcgccgcc tgcgcgcgcg gcggcaccgg
    11521 cgccgggtgc cccggccacg ggcacgaggc gcagtcgccg aacgcgaaaa cgtcggggtc
    11581 gcgggtggtc tgcagcgtcg gcttgaccac gagctggttc agtcggttgg tctcgaggtc
    11641 ggcgaggttc ttcagcacct cggcgcactt gatgcccgcg gcccacaccg tgatgtcggc
    11701 gcggaagctg tcgcccccct tggtgcgcac cgcgtcggcg gtcacctccg tgacctgggc
    11761 ggcgttgcgc acgtcgacgc cgagggactt caatacgtcg gtcgtggcct cggaaatgcg
    11821 ctccgggagc accgcgagga tgcgcgggtc ggcgttgacc agcgtcaggc gcacgtagcg
    11881 gtcggggtcg atgtgctcga ggccgtagcc ggcgagcacg cgcgtggtgt tgcgcagctc
    11941 ggcggcgagc tcgacgcccg tggcaccgcc gccgacgatg accatgtcca ggcgcccgtc
    12001 cttctggtcg ccggcgcgat agttggcctg gagacaggcg ttcacgacgc gccggtgcag
    12061 ccggtcggcc tggtcgggcg agtcgagcag caaggcgtgc tccgccaccc cggggatgtt
    12121 gaacgagttc gagacgctgc cgatcgcgat caccagcgtg tcgtagcgga tcgtcctgcg
    12181 gggaatgacc tctgcgccgt cctcgtccag cacgggcgcg accgcgagct cgcgcttcca
    12241 gcgatcgagc ccctcgagcg ccccctgcgc gaaggtgaaa tggtgccagc gcgcctgggc
    12301 gaggtagtcg agctcgttgg catgccggtc gaggctgccc gccgccacct cgtgcaggag
    12361 cggcttccac aggtgcgtgg gcgagcggtc gacaagggtc acggcggcgt gcccgcggcg
    12421 cccgaggcgg tcgcccagcc gggtggcgag ctccaggccc cccgccccgc cgccgacgat
    12481 cacgatatga tgcgggctcg ccattagaat tgtttttcgg ttctgcatct tagcgcaggc
    12541 ggggcgccgg gcaccaaagg ccgccggccc ttgtccc
//
LOCUS       DATDMW010000402         4881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_120695, whole genome shotgun sequence.
ACCESSION   DATDMW010000402 DATDMW010000000
VERSION     DATDMW010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120695"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VI319_16720"
     CDS             <1..544
                     /locus_tag="VI319_16720"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6865538.1"
                     /translation="ALVKSSPGKLNYASAGNGSTHQFCAELLKNMAHIDMVHVPYKGS
                     GPGITALLANEVSMMFSNVADIGSQIKAGKVKPLAVTSAKRAPSLPGVPTLQESGLPG
                     FEINSWFGLLVPAGTPKPIVSRLNAETRKVLAREDVKSTLGAQGLDVRASSPEEFAAF
                     IKSEMVRFARIAKAANIRAE"
     gene            633..1232
                     /locus_tag="VI319_16725"
     CDS             633..1232
                     /locus_tag="VI319_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865539.1"
                     /translation="MTKSIRLALALLVFAAASSQAQTVRVKGTLEKVNGNVLTVKTSD
                     GKEERIALADNASIVAVVKASMADIKEGTFLGSAAMPQADGTQKALEVHIFPEQMRGT
                     GEGHRPYAPVPNSTMTNGAAAGATVTGVDGGTMTVKYKGGEKKIVIPPGTPIVRYEIG
                     GHSDLRPGARFTVLAATKKSDGTLETGRINVGRDGVVPQ"
     gene            1249..3363
                     /locus_tag="VI319_16730"
     CDS             1249..3363
                     /locus_tag="VI319_16730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6865540.1"
                     /translation="MRIRSRLLVLVSAVLLPALAGSAIALGYIYEEEQDFNRASMSET
                     ARALALAIDREMARQESVLRTMAEERSLTSGHIERFKKYAMGVHRASGTHLILSGLDG
                     QQLVNTSIPAGRLPHNLEIERRFRAQHGNDVTFIADLYPAPITTGYNWALQVPVRRDG
                     RVRWLLAMVSKSADMQKLLMQQRLPARWLGTILDARGAVVARSEEPEKFLSKPVGLAL
                     AEKIADSPRGIHRGVSLDGVPSTVFWARAPVSGWTFLLSVPEEDLYGPAHRATALLAV
                     SFLLMLGLGLLAALFVARRIARPVESLRDAARRLGHDEPVAAQRSGTLELDAVGEALA
                     DASQRLRRTTADLKGQVAEAVASFEESQRALLQAQKLEALGRLTGSVANGFNNVLQAI
                     GATLHALKGGATADRAELLERCERAVASGTALSQQITAFGRERDVRVETLDTPARLRD
                     ARPLLAAALPANVRLDVDLPSALWAVSVDPAQLDVALIEIVANARDALPAGGRIVLAV
                     RNENVPAGAALPAGEYVAISVSDNGRDTQDPAHAAAFARQNGGALTVERRPGRGTTVK
                     LLLPRTAEPPAPPAPETAALSGKGRVLLVDDDERVRESVAEGLRAAGFEIDFARTADE
                     ALVRIGGGERYDAVLTDVVTSGAVSGIDLADQIRRRHPRTGVVVATGYSGRAPRVPGV
                     RTLIKPYDLRQAVEALNAAMAV"
     gene            complement(3393..4466)
                     /locus_tag="VI319_16735"
     CDS             complement(3393..4466)
                     /locus_tag="VI319_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865541.1"
                     /translation="MKDILVLGASYGSLIATKLALAGNRATLVCTRPTADLINREGTL
                     VRFPVRGRDAPLEVRSRALPGAVRARSPEDADPAAFDLVVLAMQEAQYGAPGVRALMR
                     RIARERTPCLAIMNMPPLPYLARIPGLTTHGLETCYADPSVWDGFDPARVTLASPDAQ
                     AFRPPEQPKNVLQVGLPTNFKAARFEEEGPTALLRELEAGIDAARYPEGGEALEVPVK
                     LKVHDSIFVPLAKWPMLIAGNYRCVWPNEIIPIRDAVHGNLAEARQVYEWVSALCVRI
                     GADPSDLVPFEKYAKAAESLGKPSSAARALAAGAEHIERVDRLVARIARQQGFWSDAL
                     HETVTLVDRWIERNRAARAKRAA"
     gene            4597..>4881
                     /locus_tag="VI319_16740"
     CDS             4597..>4881
                     /locus_tag="VI319_16740"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6865542.1"
                     /translation="MTLHTRKSHFLGSKLRALRKRNGLTLEDLSARCIQLDAKRAPSV
                     SYLSMIESGKRVPSEDVLALLSSLFQHAPAWFLDGSPETAPASLAERVGGA"
BASE COUNT          740 a         1777 c         1705 g          659 t
ORIGIN      
        1 cgcgctcgtg aagtcgagcc ccggcaagct caactacgcc tccgccggca acggcagcac
       61 gcaccagttc tgcgccgagc tgctcaagaa catggcgcac atcgacatgg tccacgtccc
      121 gtacaagggc tcgggccccg ggatcacggc gctgctcgcg aacgaggtgt cgatgatgtt
      181 ctccaacgtc gccgacatcg gctcgcagat caaggccggc aaggtgaagc cgctcgccgt
      241 cacctcggcc aagcgcgcgc cgagcctccc cggcgtgccg acgctgcagg agtcgggcct
      301 ccccggcttc gagatcaact cctggttcgg gctgctcgtt cccgccggga cgccgaagcc
      361 gatcgtgtcg cgcctcaacg ccgagacgcg caaggtcctc gcgcgcgagg acgtgaagag
      421 cacgctcggc gcccagggcc tcgacgtgcg cgccagctcg cccgaggagt tcgccgcctt
      481 catcaagagc gagatggtgc gcttcgcgcg catcgcgaag gcggccaata tccgggccga
      541 ataggggatt tccggccccg ccccggggcg ccgggcttat actaggcgcc cgccccgatg
      601 acgccgtcat gggacgaaac agggagaaca tcatgacgaa gagcatccgg ctggcgctcg
      661 ccctcctggt cttcgccgcc gcgtcgtcgc aggcgcagac ggtccgcgtc aaaggcaccc
      721 tcgagaaggt gaacggcaac gtcctcaccg tgaagacgag cgacggcaag gaggagcgaa
      781 tcgccctcgc cgacaacgcc agcatcgtcg ccgtggtcaa ggcctcgatg gccgacatca
      841 aggagggcac cttcctcggc agcgccgcga tgccgcaggc cgacggcacc cagaaggcgc
      901 tcgaggtcca catcttcccc gagcagatgc gcggcacggg cgaaggccac cgcccctacg
      961 ctcccgtccc gaacagcacc atgaccaacg gcgccgcggc gggcgcgacc gtcaccggcg
     1021 tcgacggcgg caccatgacg gtcaagtaca agggcggcga gaagaagatc gtcatcccgc
     1081 cgggcacgcc gatcgtgcgc tacgagatcg gcggacactc cgacctgcgc ccgggcgccc
     1141 gcttcaccgt gctcgccgcc acgaagaaga gcgacggcac gctcgagacc ggccgcatca
     1201 acgtcggccg cgacggggtc gtgccgcaat agcaccggcg ggggcgccgt gcgcatccgg
     1261 tcccgcctgc tggtgctcgt gtcggcggtg ctgctgccgg ccctggcggg ctccgccatc
     1321 gcgctcggct acatctacga ggaagagcag gacttcaacc gcgcgagcat gagcgagacg
     1381 gcgcgcgcgc tggcgctcgc gatcgaccgc gagatggcgc gccaggagtc ggtgctgcgg
     1441 acgatggccg aggagcggag cctgaccagc gggcacatcg agcggttcaa gaaatacgcg
     1501 atgggagtgc accgagcgag cggcacccat ctcatcctgt cgggcctcga cggccagcag
     1561 ctggtcaata ccagcatccc cgcaggccgc ctgccgcaca acctggagat cgagcgccgc
     1621 ttccgcgcgc agcacggcaa cgacgtcact ttcatcgccg atctgtaccc cgctccaatc
     1681 acgaccggct acaactgggc cctccaggtc ccggtgaggc gcgacggcag ggtccggtgg
     1741 ctgctcgcca tggtgtcgaa gtccgccgac atgcagaagc tgctgatgca gcagcggcta
     1801 cccgcccggt ggctcggcac catcctcgac gcccggggcg ccgtggtcgc gcgcagcgag
     1861 gagccggaga aattcctgag caagcccgtc ggcctggcgc tcgccgagaa gattgcggac
     1921 tcgccccgcg gcattcacag aggggtctcc ctggacggcg taccgagcac cgtcttctgg
     1981 gcgcgcgcgc cggtttccgg ctggacgttc ctgctgtcgg tccccgagga ggatctctac
     2041 gggcccgcgc accgggcgac cgcgcttctg gccgtgagct tcctgctgat gctcggcctg
     2101 gggctcctgg ccgccctgtt cgtcgcgcgg cggatcgcgc ggccggtcga gtcgctgcgc
     2161 gacgcggcgc ggcgcctcgg gcacgacgag cccgtcgcgg cgcagcgctc gggcacgctc
     2221 gagctcgacg cggtcggcga ggccctcgcc gatgcgagcc agcggctgcg gcggaccacc
     2281 gccgacctga aaggccaggt cgcggaggcg gtggcgagct tcgaggaatc gcagcgcgcc
     2341 ctgctccagg cgcagaagct cgaggcgctc ggacggctca ccggcagcgt cgccaacggc
     2401 ttcaacaacg tgctgcaggc gatcggcgcc acgctgcacg cgctcaaggg cggcgccacg
     2461 gccgaccgcg ccgaactgct cgagcgctgc gagcgcgcgg tggcgtccgg cacggcgctc
     2521 tcgcagcaga tcaccgcgtt cgggcgcgag cgggacgtcc gcgtcgagac gctcgacacg
     2581 ccggcgcggc tgcgcgacgc caggccgctg ctcgccgccg cgctgcccgc gaacgtgcgc
     2641 ctcgacgtcg atcttccctc cgcgctgtgg gcggtctcgg tcgacccggc gcagctcgac
     2701 gtggcgctga tcgagatcgt tgcgaacgcg cgcgacgcgc tccccgcggg cggccgcatc
     2761 gtcctcgccg tgcgcaacga aaacgtgccc gccggggccg cgcttcccgc cggcgaatac
     2821 gtcgcgatca gcgtatcgga caacggccgg gacacgcagg accctgccca cgcggcggcc
     2881 ttcgcccgcc agaacggcgg agcgctgacg gtcgagcgcc gtcccggccg cggcacgacg
     2941 gtcaagctcc tgctgccgcg cacggccgag cccccggcgc cgcccgcgcc cgagaccgcc
     3001 gcgctctcgg gcaagggccg cgtgctgctc gtggacgacg acgagcgcgt gcgcgagagc
     3061 gtcgccgagg ggctgcgcgc ggcgggcttc gagatcgact tcgcccgtac cgcggacgag
     3121 gcgctggtgc gcatcggcgg cggcgagcgc tacgacgcgg tcctcaccga cgtggtgacc
     3181 tcgggcgccg tcagcggcat cgacctcgcc gaccagatcc gccgccgcca cccgcgcacg
     3241 ggggtcgtcg tggccaccgg ctattccggc cgcgcgccgc gcgtgcccgg cgtgcgcacc
     3301 ttgatcaagc cctacgacct gcgccaggcg gtggaagcgc tgaacgcggc gatggccgtg
     3361 tagcctaagc cgcgcgcctg gcgcgagccc ttttacgccg ctcgcttcgc ccgagcggcg
     3421 cggttgcgct cgatccagcg gtcgacgagc gtcacggtct cgtgcagcgc gtccgaccag
     3481 aagccctgct gccgcgcgat gcgcgccacg aggcggtcga cgcgctcgat gtgctcggcg
     3541 ccggccgcga gcgcgcgcgc cgcggacgag ggcttgccga ggctctcggc cgctttcgcg
     3601 tacttctcga acggcaccag gtcggaagga tcggcgccga tgcgcacgca cagggccgag
     3661 acccattcgt agacctggcg cgcctcggcc aggttgccgt gcaccgcgtc gcggatcggg
     3721 atgatctcgt tgggccagac gcagcggtag tttccggcga tcagcatcgg ccacttggcg
     3781 agcggcacga agatcgagtc atgcaccttg agcttcacgg gcacctcgag cgcctcgccg
     3841 ccttccgggt accgggcggc atcgatcccc gcctcgagct cgcgcagcag cgcggtcggg
     3901 ccctcttcct cgaagcgcgc ggccttgaaa ttggtcggca gcccgacctg cagcacgttc
     3961 ttcggctgct ccggcggacg gaacgcctgc gcgtcggggc tcgcgagcgt cacccgcgcg
     4021 gggtcgaagc cgtcccacac cgagggatcc gcgtagcagg tctcgagccc gtgggtcgtg
     4081 aggccgggga tgcgcgccag gtacggcagc ggcggcatgt tcatgatcgc gaggcacggc
     4141 gtgcgctcgc gcgcgatccg gcgcatcagc gcgcgcacgc cgggcgcgcc gtactgcgcc
     4201 tcctgcatcg ccagcaccac caggtcgaag gccgcgggat cggcgtcctc gggtgagcgc
     4261 gcgcgcaccg cgccgggcag cgccctcgag cggacctcga gcggcgcatc gcgcccgcgc
     4321 accgggaagc gcacgagcgt cccctcgcgg ttgatcagat cggcggtcgg acgggtgcag
     4381 acgagcgtcg cccggtttcc ggcgagcgcg agcttggtcg cgatcaatga gccgtaggaa
     4441 gcgcccagga cgagaatatc tttcatcaca tcctccattc ggcccgcgcg gggcctggac
     4501 atcgttattc gtctgagcgc aggatagcgt tggaattcag taaatttgac tccagaaatt
     4561 tcacagtgtt aatttcacta agcttccggt gacgcgatga cgctgcacac gcggaagagc
     4621 catttcctcg gcagcaagct cagggcgctg cgcaagcgca acggcctgac gctcgaggac
     4681 ctgtcggcgc gctgcatcca gctcgacgcg aagcgcgccc cgtcggtctc gtacctgtcg
     4741 atgatcgaga gcggaaagcg cgtgccctcg gaggacgtgc tcgcgctcct gtcttcgctg
     4801 ttccagcacg cgccggcgtg gttcctggac ggcagcccgg agacggcgcc cgcctcgctc
     4861 gccgagcgcg tcggcggcgc g
//
LOCUS       DATDMW010000403         4461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_493086, whole genome shotgun sequence.
ACCESSION   DATDMW010000403 DATDMW010000000
VERSION     DATDMW010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493086"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VI319_16745"
     CDS             <1..375
                     /locus_tag="VI319_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HEY6865543.1"
                     /translation="VELVGLPEGVLPMTQAAVYLALAPKSNAALTAYAAARKLVQERG
                     ALPVPMKLRNAPTKLMKELGFGREYRYAHDEEGAYAAGENYLPDDMPRIEWYRPTDRG
                     LEQRIREKMAELRGRDEAAKKR"
     gene            372..1655
                     /gene="serS"
                     /locus_tag="VI319_16750"
     CDS             372..1655
                     /gene="serS"
                     /locus_tag="VI319_16750"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY6865544.1"
                     /translation="MIDIQLLRTDIDAVAKRLSTRGYTLDTAGFRTLEERRRKIQSET
                     EQLQSQRNAIARQVGQAKAKKDETQANALMRQGAELGEKLKQMESENASVQEKMREFV
                     SLVPNLPHESVPVGGRPEDAIEQRRWGEPRKFDFQPRDHVDIGTGLGGLDFDTAAKLA
                     GARFVVMKGAVARLHRAIAQLMLDAHTAEHGYTEVYTPYMVTAACADGVTSLAKFKDD
                     LFKVEARDLYLIPTAEYPVTNFVRDEIVPLEALPLKYVCHSPCFRSEAGAAGKDTRGM
                     IRNHQFDKVELVQIVHPSKSYEAHEQLTGHAETILKKLSLPYRVITLCTGDMGFSSAK
                     TYDLEVWLPGQKAYREISSCSNFEAFQARRMQARFRNEKGKPEPVHTLNGSGLAVGRT
                     LIAILENYQRADGGVDVPEALWPYMGGAKAIEPAG"
     gene            1660..3351
                     /locus_tag="VI319_16755"
     CDS             1660..3351
                     /locus_tag="VI319_16755"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6865545.1"
                     /translation="MDKEKKSGAEALLRILRRAGVERIFASPGSEWSPLYEALAAPHG
                     PGEVPDYLSTRHEETAVAMASGYAKITGKLPAVVIHTTVGALHERVPMVVLTGESIAF
                     GEAEGHDPGHQWLRLLADVGGPAALVERCVKFSHGANAAALLPRVIERACQLAAAEPQ
                     GPVFVSVPMEFLFEQMELGERTTVAHVPPAQAPLELIERIAEELSKAKAPVIVSEELG
                     RRPRAVEHLAAIAELLAAPVLETWNISYANFPRTHPLYAGAGGTDLVAEYVRDSDCVF
                     LVETVVPWHPPSALPRPGTKVIALGGDPLQSHLVMAGARADLIGAGEIESALALLRQR
                     LAQRVPSGTRNAAIERWRARHEERRRAQRDEARALGDRKPIDTRWVAVELDEVLPRDA
                     IVVSETITHRRPLHRYLSRVGPGRFFEGCYGGLGTGTGTALGAKVAMRDKPVVLLTGD
                     GSFNYNPVLSALGASQEHGLPILIVVFNNFGYLSQKRGVPTHFPDGWAVKSRRFAGTS
                     IAPSPDYAGLAPLFGGVGERVEDPREVRPALERGLAAIADGRLAILDVRLEPTNA"
     gene            complement(3359..3450)
                     /locus_tag="VI319_16760"
     tRNA            complement(3359..3450)
                     /locus_tag="VI319_16760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3413..3415),aa:Ser,seq:cga)
     gene            complement(3464..>4461)
                     /locus_tag="VI319_16765"
     CDS             complement(3464..>4461)
                     /locus_tag="VI319_16765"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY6865546.1"
                     /translation="AGHRPAADAAVVALARRAGGVVLGKTVTTEFATFHPGPTRNPHD
                     LEHTPGGSSSGSAAAVADHMAALAFGTQTAGSVIRPAAYCGVVGYKPTYNTLPRAGFK
                     NDADTLDTVGVLARTVEDAALFVSALAGRPELRAAAAAPARVGLCRTHQWDRAQPETV
                     AAMERAAARIAKGARVVEVALPPAFAGLLRAQLAVMWYEIARNLADEYTRHPGQLSAA
                     LRERCDKGYAMNTDEYNEAISLGRECRARLAEAFGDCDVLLAPSAPGEAPAGMPTGDP
                     VMNQVWTFLHTPCVTVPAGAGPKGLPVGVQIVGRIGDDARTLAAAQWVQGLLEKG"
BASE COUNT          696 a         1533 c         1613 g          619 t
ORIGIN      
        1 gtggagctcg tcggcctgcc cgagggcgtg ctccccatga cccaggcggc ggtgtacctg
       61 gcgctcgcgc ccaagtcgaa cgcggcgctc accgcgtacg cggcggcgcg caagctggtg
      121 caggagcgcg gcgcgttgcc cgtccccatg aagctccgca acgccccgac gaagctcatg
      181 aaggagctcg gcttcggccg cgagtaccgc tacgcgcacg acgaggaggg cgcgtacgcg
      241 gcgggcgaga actacctgcc cgacgacatg ccgcgaatcg agtggtaccg gccgaccgac
      301 cgcggcctcg agcagaggat cagggaaaag atggcggagc tgcgcggccg cgacgaagcg
      361 gcgaagaaga gatgatcgac atccagctgc tgagaaccga catcgacgcg gtcgcgaagc
      421 gcttgtcgac gcgcggatac acgctcgaca ccgcgggctt caggacgctg gaggagcggc
      481 gcaggaagat ccagagcgag accgagcagc tgcaatcgca gcgcaacgcg atcgccaggc
      541 aggtcggcca ggcgaaggcg aagaaggacg agacgcaggc gaatgcgctc atgcgccagg
      601 gcgccgagct cggggaaaag ctgaagcaga tggagtcgga gaacgcgtcg gtgcaggaga
      661 agatgcgcga attcgtctcg ctcgtcccga acctgccgca cgaaagcgtt ccggtcggcg
      721 gccgtcccga ggacgcgatc gagcagcgcc gatggggcga gccgcgcaag ttcgatttcc
      781 agccgcgcga tcacgtcgac atcggcaccg gcctcggcgg gctcgatttc gacaccgcgg
      841 cgaagctggc gggcgcgcgc ttcgtcgtga tgaaaggcgc cgtcgcgcgg ctgcaccgtg
      901 cgatcgcgca gctgatgctc gacgcgcaca cggcggagca cggctacacc gaggtctata
      961 cgccctacat ggtgacggcc gcctgcgccg acggcgtgac cagcctcgcg aagttcaagg
     1021 acgacctgtt caaggtcgag gcccgcgatc tgtacctgat cccgacggcg gaatatcccg
     1081 tcaccaactt cgtgcgcgac gagatcgtgc cgttggaggc tctgccgctc aagtatgtct
     1141 gccattcgcc gtgctttcgc tcggaggccg gcgccgcggg caaggatacg cgcggcatga
     1201 tccgcaatca ccagttcgac aaggtggaac tggtgcagat cgtgcaccca tcgaaatcgt
     1261 atgaagccca tgagcagctg accgggcacg cggagaccat cctgaaaaag ctctcgcttc
     1321 cttaccgcgt gatcacgctg tgcaccggcg acatgggctt ctcctccgcg aagacctacg
     1381 acctcgaggt ctggctcccg ggccagaagg cgtaccgcga gatctcctcg tgcagcaact
     1441 tcgaggcgtt ccaggcgcgg cgcatgcagg cgcgctttcg gaacgagaag ggcaagcccg
     1501 agccggtgca cacgctgaac ggatcagggc tcgctgttgg ccgtacgctg atcgcgatcc
     1561 tggagaacta ccaacgcgcg gacggcggcg tcgacgtgcc cgaggcgctg tggccgtaca
     1621 tgggcggcgc gaaggcgatc gagccggcgg ggtgacgcga tggacaaaga aaagaagagc
     1681 ggcgctgaag cgctgctgag gatcctgcgg cgcgcgggcg tcgagcgcat cttcgcgtcg
     1741 ccgggctcgg agtggtcgcc gctgtacgag gcgctcgccg cgccgcacgg cccgggcgag
     1801 gtcccggatt acctctccac gcgccacgaa gagacggcgg tcgcgatggc gagcggctac
     1861 gcgaagatca ccggcaagct gcccgccgtc gtcatccaca cgacggtcgg cgcgctgcac
     1921 gagcgcgtgc cgatggtggt gctcacgggc gagtcgatcg cgttcggcga ggcggaaggg
     1981 cacgacccgg gccatcagtg gctgcggctg ctcgccgacg tcggcggtcc cgcggcgctg
     2041 gtcgagcgct gcgtgaagtt cagccacggc gcgaacgccg cggcgctgct gccgcgggtg
     2101 atcgagcgcg cgtgccagct cgcggccgcc gagccgcaag ggcccgtgtt cgtctcggtg
     2161 cccatggagt tcctgttcga gcagatggag ctcggcgagc gcacgacggt ggcgcacgtc
     2221 ccgccggctc aggctccgct cgagctcatc gagcgcatcg ccgaggagct gtcgaaggcc
     2281 aaggcgcccg tgatcgtcag cgaggagcta gggcgccgtc cgcgcgccgt cgagcatctc
     2341 gccgcgatcg cggagctgct cgcggcgccg gtgctggaga cgtggaacat cagctacgcc
     2401 aacttccccc gcacgcaccc gctgtacgcg ggagccggcg gcaccgatct cgtcgcggaa
     2461 tacgtccgcg actccgattg cgtgtttctg gtcgagacgg tcgttccctg gcatccgccc
     2521 tcggcgctgc cccgccccgg gacgaaggtg atcgcgctcg ggggcgatcc gctccagagt
     2581 catctcgtca tggcgggcgc gcgcgccgac ctgatcggcg ccggcgagat cgagtctgcg
     2641 ctcgcgctgc tgcgccagcg cctcgcgcag cgcgttccct cgggaacccg caacgcggcg
     2701 atcgagcgct ggcgcgcgcg ccacgaggag cgccgccgcg cccagcgcga cgaggcgcgc
     2761 gcgctcggcg accgcaagcc gatcgacacg cgctgggtcg ccgtggagct cgacgaggtg
     2821 ctgccccggg acgcgatcgt cgtcagcgaa accatcacgc atcggcggcc gctccaccgg
     2881 tacctttcgc gcgtcgggcc gggacggttc ttcgagggct gctacggcgg gctcggcacc
     2941 ggcaccggca cggccctcgg cgcgaaggtc gcgatgcgcg acaagccggt ggtgctgttg
     3001 accggcgacg gctcgttcaa ctacaacccg gtgctctcgg cgctcggcgc gtcgcaggag
     3061 cacgggctgc cgatcctgat cgtggtgttc aacaacttcg gctacctctc gcagaaacgc
     3121 ggcgtgccga cgcatttccc ggacgggtgg gcggtgaagt ccaggcgctt cgccgggacg
     3181 tcgatcgcgc cgagccccga ctacgcgggc ctcgcgccgc tgttcggcgg cgtcggcgag
     3241 cgggtggagg atccgcgcga ggtgcgcccg gcgctcgagc gcggcctggc ggcgatcgcc
     3301 gacgggcggc tcgcgatcct ggacgtgcgg ctggagccta cgaacgcatg agaagaagtg
     3361 gcggagaggg tgggattcga acccacgtgc cgggaaaccc gaccatctga tttcgagtca
     3421 gcgccgttat gaccgcttcg gtacctctcc ggagccgcgt attctaacct ttctccaaca
     3481 agccctggac ccactgagcc gcggcgagcg tcctcgcatc gtcgccgatc cggccgacga
     3541 tctgcacgcc gacgggtagg cctttcgggc cggcgcccgc ggggacggtg acgcagggcg
     3601 tgtgcaggaa ggtccacacc tggttcatca ccgggtctcc ggtcggcatc ccggccgggg
     3661 cctcgcccgg cgccgacggc gcgagcagca cgtcgcaatc gccgaaggcc tccgcgagcc
     3721 gcgcgcggca ttcgcggccg agcgagatcg cctcgttgta ttcgtcggta ttcatcgcat
     3781 agcccttgtc gcagcgctcg cgcagagccg cgctcagctg cccggggtgg cgcgtgtact
     3841 cgtcggcgag gttgcgggcg atctcgtacc acatgaccgc gagctgcgcc cggagcagcc
     3901 ccgcgaacgc cggcggcagc gcgacctcga cgacgcgggc gcctttggcg atccgcgccg
     3961 ccgcacgctc catggcggcg acggtttcgg gctgcgcccg gtcccattga tgcgtgcggc
     4021 acagcccgac gcgggccggg gcggcggcgg ccgcgcgcag ctcgggccgg ccggcgagcg
     4081 ccgatacgaa cagcgccgcg tcctcgaccg tacgcgcgag cacgccgacc gtgtcgagcg
     4141 tgtccgcgtc gttcttgaag ccggcgcgcg ggagcgtgtt gtacgtgggc ttgtagccca
     4201 cgacgccgca gtaggccgcg ggccggatga ccgatcccgc ggtctgcgtc ccgaacgcga
     4261 gcgccgccat gtgatcggcg accgccgcgg ccgatccgct cgaggatccg cccggcgtgt
     4321 gctccagatc gtgcggattg cgcgtcggcc ccgggtggaa ggtcgcgaac tcggtcgtga
     4381 ccgtcttccc gagcaccacg ccgcccgcgc gccgcgcgag cgcgacgacg gcggcgtccg
     4441 ccgcgggccg gtggcccgcg t
//
LOCUS       DATDMW010000404         4021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1739485, whole genome shotgun sequence.
ACCESSION   DATDMW010000404 DATDMW010000000
VERSION     DATDMW010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739485"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="pgeF"
                     /locus_tag="VI319_16770"
     CDS             complement(<1..722)
                     /gene="pgeF"
                     /locus_tag="VI319_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY6865547.1"
                     /translation="MSAAFDPDWIVPDWPAPGGVRALVTTRSGGVSAGPYATLNLGSR
                     VGDDPACVARNRAILRAVLPSEPRWMKQVHGTRALRADAAAHDVEADAAVATSAGEVC
                     AVLVADCMPVLLCDDEGSVVAAAHAGWRGLSAGVVEAAVAAMRVPARRVLAWLGPAIG
                     PSAYEVGADVREAFVGRDAGAAAAFAPRGQGKFLADLAALARRRLSACGVGRVYGGSW
                     CTASDPRFFSFRRDRITGRMAA"
     gene            complement(719..1663)
                     /locus_tag="VI319_16775"
     CDS             complement(719..1663)
                     /locus_tag="VI319_16775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY6865548.1"
                     /translation="MSNENRPADYSPGSLRRVPESLAGLRLDQALARMFPEHSRSRLQ
                     HWLREGHIRLDSRAAKPKQKIWGGEQIEIAPQPDPSGSTHRPERIPLDVVHEDDAVLV
                     LNKPAGLVMHPGSGNWKGTLLNALLEYAPATAAVPRAGIVHRLDKDTSGLVVVAKTPE
                     AQTSLVRQLQARTVRREYLALVHGAVKSDGEISGAVGRHPRERTRMAVVPGGKPALTR
                     YRVAARLEGATLLHCSLATGRTHQIRVHMQSIGHPLVGDPVYRDRRRSRADPRLAAFP
                     RQALHAARLSFVHPVSAKPVSFEAPMPEDLRALLDGLA"
     gene            1691..2485
                     /locus_tag="VI319_16780"
     CDS             1691..2485
                     /locus_tag="VI319_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY6865549.1"
                     /translation="MKRSVAAAACSVLALALAGCGLFPDKVDPADTWPANRLYKEAKD
                     ELDGGNYDQAIKYYEKLESRYPYGRYAQQAQLEIAYAYYKQGEQASGVAAIDRFIKLH
                     PNHANVDYAYYLKGLIYFNEDQGFLGKISDQNPAERDPKAPREAFDAFRELVQRYPES
                     KYAPDAVARMKYLVNALASHEVFVARYYVKRGAWVAAINRAQYALQNYPESPACEEAL
                     FIMVQAYDALGMKQLSGDAARVLRQNFPKTRYFASEDVTGPWWKVW"
     gene            complement(2457..>4021)
                     /locus_tag="VI319_16785"
     CDS             complement(2457..>4021)
                     /locus_tag="VI319_16785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY6865550.1"
                     /translation="ADLQRIGRFARVSASGDRGELAEVPEDAPAWALATSTRENCLGQ
                     SCPRYADCFVMAARRRALAADLVVVNHHLFFADAALKDDGMAELLPACNTVIFDEAHQ
                     LPETATLFFGETVSSAQLLELARDARREALAGAPDVPALAERIAAVEKAARDLRLSLP
                     AEGARYAAQALDARREFAAALDGVHEALAALGAGLEAVAERAEGIARCAERAGAALDL
                     LLRWREGDRERLIRWVEAYAHSLALNATPLSVALLFRRQLEGRPRAWVFVSATLAVAG
                     DFGLYCAEMGLEDADTACWDSPFDYARNATLYVPQGLPDPSSPEHTRAVVQAALPVLR
                     ASRGRAFLLFTTLRAMRLAHELLREAFAREGLAYPLLLQGEGSRSELLERFRGLGDAV
                     LVGSASFWEGVDVRGEALSLVVIDKLPFAPPDDPVLAARLAAFEREGRSAFAEYQLPR
                     AAIALKQGAGRLIRDEADRGVLMICDPRLFSRTYGRRLLASLPPMRPTRDEAEAVAFF
                     SGSATRPSTTDP"
BASE COUNT          615 a         1463 c         1414 g          529 t
ORIGIN      
        1 aaggccgcca ttctgccggt gatccggtcg cggcgaaagg agaagaagcg cggatcgctc
       61 gccgtgcacc agctcccgcc gtagacgcgg ccgaccccgc aggccgagag ccggcggcgc
      121 gcaagcgccg cgagatcggc caggaacttg ccctggccgc gcggggcgaa tgctgcggcg
      181 gccccggcgt cgcggccgac gaacgcctcg cggacgtcgg cgcccacctc gtacgcgctc
      241 ggcccgatag cgggcccgag ccatgcgagc acgcggcgcg ccggcacgcg catggcggcg
      301 acggcggcct cgacgacacc cgcgctcagg ccgcgccatc cggcgtgcgc cgccgcgacg
      361 acgctcccct cgtcatcgca aaggagcacc ggcatgcaat ctgcgacgag caccgcgcac
      421 acctcgcccg cggaagtggc caccgcggcg tcggcctcga cgtcgtgcgc ggccgcgtcg
      481 gcgcggagcg cgcgggtgcc atgcacctgc ttcatccagc gcggctcgga cggcaggacg
      541 gcgcgcagga tggcgcggtt gcgcgccacg cacgcgggat cgtcgccgac tcgcgagccg
      601 aggttgagcg tcgcgtaggg accggcgctg acgccgcccg agcgcgtcgt gacgagcgca
      661 cgcacgccgc cgggcgcagg ccagtcggga acgatccagt cgggatcgaa ggccgcgctc
      721 acgcgagccc gtccagcagc gcgcgcagat cctcgggcat cggcgcctcg aagctgacgg
      781 gtttcgccga gacgggatgc acgaacgaga gcctcgccgc gtgcaacgcc tgtctcggaa
      841 acgcggcgag gcgcggatcc gcgcgcgaac gccggcggtc gcggtacacg ggatccccga
      901 ccagcggatg cccgatggat tgcatgtgca cgcggatctg gtgcgtgcgg cccgtcgcca
      961 ggctgcaatg caggagcgtg gcgccctcca ggcgcgcggc cacccggtag cgcgtaagcg
     1021 ccggcttgcc cccgggcacg accgccatgc gcgtgcgctc acgcggatgg cgcccgaccg
     1081 cgcccgaaat ctcgccgtcc gacttgacgg cgccgtgcac gagcgcgaga tactcgcggc
     1141 gcaccgtgcg cgcctgcagc tggcgcacga ggctcgtctg cgcctcgggc gtcttggcga
     1201 ccacgaccag gccgctcgtg tccttgtcga ggcggtgcac gatccccgcg cgcggcacgg
     1261 ccgcagtcgc gggcgcgtat tcgagcagcg cgttgagcag cgtgcccttc cagttgccgc
     1321 tgcccggatg catcaccagc ccggcgggct tgttcagcac gagcaccgcg tcgtcctcgt
     1381 gcacgacgtc cagcgggatg cgctcgggcc ggtgcgtgct cccggaggga tcgggctggg
     1441 gcgcgatctc gatctgctcg ccgccccaga tcttctgctt cggcttcgcg gcgcgcgaat
     1501 ccagtcggat gtggccctcg cgcagccagt gctgtagccg gctgcgcgaa tgctcgggga
     1561 acatgcgcgc gagcgcctgg tccaggcgca gtcccgcgag cgattcgggc actcggcgca
     1621 acgagcccgg gctataatcg gcgggccgat tttcgttgct cattctggcc gccgcatcgt
     1681 cgtgtcacgc atgaaacgta gtgtagccgc agccgcctgc tccgtcctcg cgctggcgct
     1741 ggccgggtgc gggctgttcc ccgacaaggt ggatcccgcc gacacctggc ccgcgaaccg
     1801 gctgtacaag gaagcgaagg acgagctgga cggcggcaac tacgaccagg cgatcaaata
     1861 ctacgaaaag ctcgagtcgc gctatccgta cggacgctac gcgcagcagg cgcagctcga
     1921 gatcgcctac gcctactaca agcagggcga gcaggcgtct ggcgtggccg cgatcgaccg
     1981 cttcatcaag ctgcacccca atcacgccaa cgtcgactac gcgtattacc tgaaaggcct
     2041 gatctacttc aacgaggacc agggcttcct cgggaagatc tccgaccaga atcccgccga
     2101 gcgcgacccg aaggcgccgc gcgaagcgtt cgacgccttc cgcgagctgg tgcagcgcta
     2161 tcccgaaagc aagtacgcgc cggacgcggt ggcccgcatg aaatacctcg tgaacgcgct
     2221 cgcgtcgcac gaggtcttcg tcgcgcgcta ttacgtgaag cgcggcgcct gggtcgccgc
     2281 gatcaaccgc gcccagtacg ctttgcagaa ctaccccgag tcgccggcgt gcgaggaagc
     2341 gctgttcatc atggtgcagg catacgacgc gctcggcatg aagcagctga gcggcgacgc
     2401 cgctcgcgtc ctgcgccaga acttcccgaa gactcgctac ttcgcctccg aggacgtcac
     2461 gggtccgtgg tggaaggtct ggtagcggag ccggagaaga acgcgacggc ctcggcttcg
     2521 tcgcgcgtcg gtcgcatcgg cggcaggctg gcgagcagcc ggcgtccgta ggttctcgag
     2581 aacaagcgcg ggtcgcagat catcagcacg ccgcgatcgg cttcgtcgcg aatcagccgc
     2641 ccggctccct gcttcagcgc gatcgccgcg cgcggcaact ggtattcggc gaacgcgctg
     2701 cgtccttctc gctcgaaagc cgcgaggcgc gcggcgagca ccgggtcgtc gggcggcgcg
     2761 aagggcagct tgtcgatgac cacgagcgac agcgcctcgc cgcgcacgtc gacgccttcc
     2821 cagaagctcg cgctgccgac gaggaccgcg tcgcccagcc cccggaagcg ctccagcagt
     2881 tcgctgcgcg agccttcgcc ctgaagcagc agcggatacg cgagtccctc gcgcgcgaac
     2941 gcctcgcgca gcagctcgtg cgcgagacgc atcgcgcgca gggtggtgaa caggaggaag
     3001 gctcgcccgc ggctggcgcg cagcacgggc agcgccgcct gcaccaccgc gcgcgtgtgc
     3061 tcggggctgc tcgggtcggg cagcccctgc ggcacgtata gcgtcgcgtt gcgcgcgtag
     3121 tcgaacgggc tgtcccagca cgcggtgtcc gcgtcctcca gccccatctc cgcgcaatag
     3181 agcccgaagt cgcccgccac cgcgagggtc gcggacacga atacccacgc gcgcggccgc
     3241 ccctcgagct gcctgcgaaa gagcagcgcg accgacagcg gcgtcgcgtt gagggcgagc
     3301 gagtgcgcgt acgcctcgac ccaccggatc aggcgctcgc ggtcgccttc gcgccagcgc
     3361 agcagcaggt cgagcgccgc gccggcgcgc tcggcgcagc gcgcgatgcc ttcggcgcgc
     3421 tcggcaacgg cttcgaggcc cgcgccgagc gcggcgagcg cttcgtgcac gccgtcaagc
     3481 gcagcggcga actcgcggcg tgcgtcgagc gcttgcgcgg cgtagcgggc gccttcggcc
     3541 ggcaacgaca gcctcagatc gcgcgccgcc ttttcgacgg ctgcgatccg ctccgctagc
     3601 gcgggcacat ccggagcgcc ggcgagcgcc tcacggcgtg cgtcgcgcgc gagctcgagc
     3661 agctgcgcgc tggagacggt ctcgccgaag aacagcgtcg ccgtctccgg gagctggtgc
     3721 gcctcgtcga agatcaccgt attgcacgcg ggcaggagct ccgccatccc gtcgtccttc
     3781 agcgccgcgt cggcgaagaa cagatggtga ttcacgacga caagatcggc cgcgagcgcg
     3841 cgccgccggg ccgccatgac gaagcagtcg gcatagcgcg gacacgattg tcccaggcag
     3901 ttttcgcgcg tcgacgtcgc gagcgcccac gcgggtgcgt cctccggaac ctccgccagc
     3961 tcgccgcggt cgccgctcgc ggacacgcgc gcgaagcgcc cgatgcgctg cagatccgcc
     4021 g
//
LOCUS       DATDMW010000405        14273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1491103, whole genome shotgun sequence.
ACCESSION   DATDMW010000405 DATDMW010000000
VERSION     DATDMW010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491103"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VI319_16790"
     CDS             complement(<1..520)
                     /locus_tag="VI319_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865551.1"
                     /translation="MFKSLLGALTASLALVAPAQAQEPIKIGMVLEMSGAFADFGMQM
                     TNGARAYMKAHGDTVAGRKVVLIVKDTTGAPDVARRVAQELLTNDKVDFLAGFGLTPS
                     ALSVAQLATEAKKPMIVMNAATSIITTRSPYIARVSFTLPQVTAPLATWAVKNGVTRA
                     YTLVADYGPGIDA"
     gene            complement(541..1707)
                     /locus_tag="VI319_16795"
     CDS             complement(541..1707)
                     /locus_tag="VI319_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6865552.1"
                     /translation="MKRIEVVVPVLVAALAAGAAFAQEPIKVGMLAEMSGPFADIGRQ
                     IVNGARAYVKAHGDTVAGRRVEIIVKDTTGMAPEIAKRQAQELIVNDKVDFLAGFGLT
                     PNALAVAPLATEAKKPMIVMNAATSIITTRSPYIVRVSMTLPQVTAPLATWAARNGIR
                     EVYTLVSDYGPGIDAETQFRKTFTEAGGKIVGGVRTPPNNPEFAPYLQRIKDARPQAV
                     FIFLPQGAQPVAVMKGFAERELDKAGIRLIATGDVTEDSLIETMGDAPLGVVTTHQYS
                     VAHASAQNRAFVKAYAEIDARSRPNFMAVGAWDGMAAIYETARRLNGKIDGDRAIAAL
                     KGMRIDSPRGPISIDPETRDVVQTIYIRRVEKLKDGKLYNVEFDQIDNVKDPGK"
     gene            complement(1729..2955)
                     /locus_tag="VI319_16800"
     CDS             complement(1729..2955)
                     /locus_tag="VI319_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6865553.1"
                     /translation="MPEHLGWPFFDDGHRALAREFEHWAADALPALVGGDDERSEAIY
                     ACVGRLVAELGKAGLLRACVPAAYGGLRDKVDVRSLCIARETLARASGLADFALAMQG
                     LGSVPITLHGSEAQKRAFLPRVASGRAIAAFALSEFDAGSDVAAMTTTAVRDGDGWRL
                     DGVKTWISNGGLADHYVVFARSGEAPGAKGISAFIVDADAHGLDASRRIDVIAPHPLA
                     TVRLDGCRIAGDRLLGRPGEGFRIAMATLDVFRSTVGAAALGFARRAMDEAVSRAKDR
                     RLFGRTLAEFQLTQAKAGDMATAIDASALLVYRAAWTKDVLGTRVTREASMAKLYATE
                     SAQQVIDSALQLFGGLGVVRGVTVERLYREIRALRIYEGASEVLKLIVGAKALEAAAT
                     APQTPPVPEAAAATAT"
     gene            complement(2961..3791)
                     /locus_tag="VI319_16805"
     CDS             complement(2961..3791)
                     /locus_tag="VI319_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEY6865554.1"
                     /translation="MQGRVTNKGLGGYQAEHFSYAVASRVATITLNRPERKNPLTLES
                     YAELRDLFRRMVYADDVKAVVITGAGGNFCSGGDVHDIIGKLVKMEMPDLLKFTRMTG
                     DLVKAMRACPQPIVAAIDGVCTGAGAMIACASDLRYATARSKLAFLFVRVGLAGADMG
                     ACTLLPRIVGLSRAADLLYTGRVVGGEEAERFGFHNRLVEPEKLLEEATAMAVSLADG
                     PTFGHAMTKTMLYQEWSHGLDECIEAEAQAQAICMKTRDFERAYNAFAAKQKPVFEGN
                     "
     gene            complement(3802..4548)
                     /locus_tag="VI319_16810"
     CDS             complement(3802..4548)
                     /locus_tag="VI319_16810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEY6865555.1"
                     /translation="MKFQDKIAVVTGGASGIGQATAEALAEEGATVCIGDVARDRGEA
                     AAAAIRKNGRKAEYFYLDLTNADSINAFKDAVLKKFGRVDILVNGAGWGKTSPFVEGD
                     DAFWEKVINLNFVGPMRLTKALLPQMMERKSGRIVSISSDAGRVGSLGETVYSGAKAG
                     LIGFTKGLAREGARYNVTANCVCPGPTDTPLLAAVPEKYREAFIKAIPMRRFGKPQEV
                     AAAVAYFAAPEAEYVTGQVLSVSGGLTMVG"
     gene            complement(4545..5327)
                     /locus_tag="VI319_16815"
     CDS             complement(4545..5327)
                     /locus_tag="VI319_16815"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY6865556.1"
                     /translation="MIAIERAGRVLVATLSRPPVNALDAALVRRLGEVLDEAVGDGFS
                     VLHFRSDQKTFCAGADLALMRSCFAREDGADAMLEVVIQMQRVYARIEAAPVVSIAEI
                     GGAALGGGLELALACDLRIAATDAPLGLPEAQLGLVPGAGGTQRLTRACGPAAAKRLI
                     LGAEVIDGAEAQRLGVVQWCARRNDLADKARSVAQNIAALPRAALAAAKHCISAYGHP
                     TRNGFVEELAGTRALYGDPETRRKVTDFLNRSKSENRVKETS"
     gene            complement(5324..6919)
                     /locus_tag="VI319_16820"
     CDS             complement(5324..6919)
                     /locus_tag="VI319_16820"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY6865557.1"
                     /translation="MNDRLQPSLAPAAGARPAPVRDLRDWLERVDAMGELVRVQHPVS
                     RDEEMSAIGYLLAKQDPSPAVLFECASGFEKSPIGARLLWNIIGPSRRRTALTLEEPV
                     DTPAVELIRRVKDKLKRRIPPLEVSPHGAPVYEHTVTGRDIDLESLPIPRHWPLDGGR
                     YAGTADAVITRDPDSGYLNVGTYRMMLQGKSEVGLYLSPGKDARLHITRAWQKGEPIQ
                     VAAAWGIDPLFMLVGSQTFPKNVSEYEFAGGIKGEAVRVVRGTTTDLLVPADAELVIE
                     GIIRPNSVRAEGPFGEFPGYYGRPEAGAPLVEVTAVHYRSSPILTNALMADYPSCEQS
                     GFFSIIRSAKIWDDLDKLGVPGIAGVYSHPAAAGGFGMTVVSLEQRHAGHAAQVLALA
                     AQVPGGAYYTKWIIAVDEDIDPTDMDQVIWAMASRCNPVDDIDILRNTWSTWLDPTQN
                     PPENRPYGSKALINACKDHRHLPEFSKRTALRKSTYERIAARWKELGLPGTAPSVRAF
                     EQDSAVVYHETGSFEPHRAKAAP"
     gene            complement(6916..8436)
                     /locus_tag="VI319_16825"
     CDS             complement(6916..8436)
                     /locus_tag="VI319_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6865558.1"
                     /translation="MPMNAAESLLSAGDDARTALECGNARVSYRDLRNAVARAAAGWR
                     RLGLETGDRVLIFAPDSIDWVIAYLGAMWAGGVAVGLNSRLFDKDLGVVLDDSGARFI
                     VCDEAGLKTLARLGRAEGSSPRIVTIAEARAIRDAVPVRSPEQLSDDSPAFWIYTSGT
                     TGVPKGVMHAQRAVVETANFASTILGVGSDARLYATSKLFFAYALGNALFAGLRLGAT
                     VILDPEWPTAERTARIVERYAPTVMFSVPTLYSKLLQAGLAPGLSAAGVRHFVSAGEA
                     FPPSLRDSWRQATGVSPVSGFGASETIVLILYCTADETLLTQSPDCDLRPRDSDDHSV
                     GAPGRIWVRHPSVALGYWKRPQAQADSFEAGWFSPGDLFRPASSTSWEFCGRDDDMLK
                     ISGQWVSVIEIEQALVAACAGTVQQLAAVGYTGADGLGAIALFAVAAPGHEAEARARI
                     DAGIAALPKIKRPRLVKWLDELPVTATGKLQRRKLRETHLPAAPAVAPEVLAKVTS"
     gene            8621..9601
                     /locus_tag="VI319_16830"
     CDS             8621..9601
                     /locus_tag="VI319_16830"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865559.1"
                     /translation="MKPSPLRVVGLGLIALILAGAAAAQAPAAYPSRPIKIIVPFPPG
                     NTMDIMSRLIGPKMSERLGQQILVENRPGASGMLGLDLVAKAPADGYTVGAGQGGNMV
                     VLPHTSRNIPYDPLKDFAPIAVSTTNYLAIVANPQAPFRDIAGMIAYAKANPGKLTVA
                     TNGEGGFPHLAFEHLRLLGGFTFTHVPYTGSAAIATDVIGGQVQVGIDGITGLTPHVK
                     SGRLRLLAITNRKRVPLWPDTQAAAEAVPGYESGGWFGYVAPAATPRAIIARLNAEIN
                     RAMQLPDVSDKLVSAGLIVVAESPEYFANVIRSDYAKYGKLVRDIGFTPQ"
     gene            complement(9611..10579)
                     /locus_tag="VI319_16835"
     CDS             complement(9611..10579)
                     /locus_tag="VI319_16835"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865560.1"
                     /translation="MRLASKCVRLLLLALVAQTALAQSDYPRRPIKMILPNAPGSSND
                     TLSRVLGARMGDVIGQQIVIENQAGASGLIGMESTRNSAPDGYTIASASPSGMTIAPN
                     LRKSLPYDPLKDFEFVSMFAVLPNMLVVTPSLPVKSTKELIDWCKSHPGQVNMASAGP
                     GSQSHLAGAMMQVMGHFESVHVPYKGGGASVLSVMTGESQWTITPASAAIGHVRSGKL
                     RALAQSLPQRTPLLPGLPPVAETIPGFSYSAWNGLVTPKGTPRPVIDKLRAALVKTLE
                     LPEVKAAIEKQGAEIMTDTPEEFRKLVEDEIRETGKVVKAAGLKIQ"
     gene            complement(10605..11570)
                     /locus_tag="VI319_16840"
     CDS             complement(10605..11570)
                     /locus_tag="VI319_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HEY6865561.1"
                     /translation="MTHAVLCLRPRADFERVDALPPASLAVSYARPDDPQLSVMIARS
                     RALVIPALGAKLAAPLFEGARLALVQVTGAGFDRLDAAAMKRLGIPVANVPGGSNAAL
                     AEYAVTAASLLLRRFAWADAEVRAGNYAAFRARMLADNLSGIEGLTVGLIGLGTIGTA
                     VARAFERSGCTIVYFDPAPREAPGARRVGLDELLAVSDVVSVHVPLLPETTGLIGERE
                     LAAMKPGAVLVQASRGGVVDEAALARALESGRLGGAAVDVYSSEPPAPDHPLLALRGD
                     AARRLLLTPHVAGVTRQSSALLFRSAWANVERVLVKGEPPHHRVY"
     gene            complement(11567..11935)
                     /locus_tag="VI319_16845"
     CDS             complement(11567..11935)
                     /locus_tag="VI319_16845"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6865562.1"
                     /translation="MARVVKPAQARELGLPGRRSLEIVSGASGSHAVTLRRVEIAVPQ
                     PGDKPRGRHFHADTEECIYVLSGQGRTESDSGDHELRAGDTILIPAGETHVTRNTGGE
                     PLVLLCFFPTARVVNRDLGK"
     gene            complement(11935..12741)
                     /locus_tag="VI319_16850"
     CDS             complement(11935..12741)
                     /locus_tag="VI319_16850"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY6865563.1"
                     /translation="MNPPSRYFQELTQPEIAAQFRRNPLVILPAGSVEQHGPHLPTGT
                     DIFASNVIGHAVAERMDGVVLPGGPLGVTPMHMPFEGTITLTPETYMRVVVETCAATA
                     KHGAKYLLILNWHEGNIPSLAIAAESLHREHGMTVLTVQACYVAADLYGKDCGGLTHG
                     GEIEALAVLACRPDLVHLDRIDYSSDHSHGRKWDKLRRTRSFQPVLRDIRTIAPTGWY
                     GAPEHATVDKGVKMLADIADAIAKEARELFRMLDEAQGGTAEVKHLKVAL"
     gene            complement(12755..13843)
                     /locus_tag="VI319_16855"
     CDS             complement(12755..13843)
                     /locus_tag="VI319_16855"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6865564.1"
                     /translation="MDSPVNVGIVGLGRWAKVLARAAAQSDKIRIVSATSRSEDKRAA
                     FAAEFGVAPVADLKALLADDRIRGVILTVPNEQHWPLARQVAAAGKHVYTEKPIANTL
                     EDGLQMADLEREHGITVTVGHSARLMAGIRAIRAKIESGELVRVAVIEANFSNERALE
                     LTPKTWRWYKDKAPGGPLSQLAIHHFDVLHYLGGEMTEVSSTASKLSPVGAEVDDQSM
                     TTVRFADGKIGYVGSCWTSPGVFALRVFSSKGLMHYEIDFGTWDTPHLLHKSATLYIQ
                     RGKDGYARREVLAVPESDMFRTELEMFAESCRTGRSGELTAHNGNVALAAVYAALRSI
                     ERRGQAVTLAEVVDETRARIAGRAQRAA"
     gene            13916..>14273
                     /locus_tag="VI319_16860"
     CDS             13916..>14273
                     /locus_tag="VI319_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY6865565.1"
                     /translation="MDPLETLRHDVALANRVIERFGLANAFGHVSARIPGTSTFYFPT
                     RRSPGLADEKRLLVLDTDGNIVSGDGIPNSELWIHARIYAARPDVGGVAHAHPGACVC
                     LTLIGQPLRVVHNMGGA"
BASE COUNT         1999 a         5013 c         5088 g         2173 t
ORIGIN      
        1 cggcgtcgat gcccgggccg tagtcggcga ccagcgtgta cgcgcgggtg acgccgttct
       61 tcaccgccca ggtcgcgagc ggcgcggtca cctgcggcag cgtgaacgag acgcgcgcga
      121 tgtacggcga gcgcgtggtg atgatcgacg tcgccgcgtt catgacgatc atcggcttct
      181 tcgcctcggt cgcgagctgc gccaccgaga gcgcgctcgg cgtgagcccg aaccccgcga
      241 gaaagtcgac cttgtcgttg gtcagcagct cctgcgccac gcggcgcgcg acgtcgggcg
      301 cgccggtcgt gtccttcacg atcagcacca ccttgcggcc ggcgacggtg tcgccgtgcg
      361 ccttcatgta cgcgcgcgcg ccgttcgtca tctgcatgcc gaaatcggcg aaggcgccgc
      421 tcatctcgag caccatgccg atcttgatcg gctcctgcgc ctgggcgggt gcgacgagcg
      481 ccagcgaagc ggtcaatgct cccagcagcg acttgaacat ggtcttctcc tcttgccggg
      541 tcacttcccc ggatccttga cgttgtcgat ctggtcgaac tcgacgttat agagcttgcc
      601 gtccttcagc ttctccaccc ggcggatgta gatggtctgc accacgtcgc gcgtctccgg
      661 gtcgatcgag atcggcccgc gcgggctgtc gatcctcatg cctttcagcg ccgcgatcgc
      721 gcggtcgccg tcgatcttcc cgttcaggcg ccgcgcggtc tcgtagatcg ccgccatgcc
      781 gtcccaggcg ccgaccgcca tgaagttcgg acggctgcgc gcgtcgatct cggcgtacgc
      841 cttcacgaag gcgcggttct gcgcgctcgc gtgcgcgacc gagtactggt gcgtggtgac
      901 gacgcccagc ggcgcgtccc ccatggtctc gatcaggctg tcctcggtga cgtcgccggt
      961 ggcgatcagg cggatccccg ccttgtcgag ctcgcgctcg gcgaatccct tcatcacggc
     1021 gaccggctgc gcgccctgcg gcaggaagat gaagacggcc tgcgggcgcg cgtccttgat
     1081 gcgctgcagg tacggcgcga actcgggatt gttcggcggc gtgcgcacgc cgccgacgat
     1141 cttgccgccc gcctcggtga aggtcttcct gaactgcgtc tcggcgtcga ttcccgggcc
     1201 gtagtcggag acgagggtgt agacctcgcg gatgccgttc ctcgcggccc aggtcgcgag
     1261 cggcgcggtc acctgcggca gcgtcatgga cacgcgcacg atgtacggcg agcgcgtggt
     1321 gatgatcgag gtcgcggcgt tcatgacgat catcggcttc ttcgcctcgg tcgcgagggg
     1381 cgcgaccgcg agcgcgttgg gcgtgagccc gaagccggcg aggaaatcga ccttgtcgtt
     1441 gacgatgagc tcctgcgcct ggcgcttcgc gatctcgggc gccatcccgg tcgtgtcctt
     1501 gacgatgatc tcgacgcgcc ggccggccac cgtgtcgccg tgcgccttca cgtaggcgcg
     1561 ggcgccgttc acgatctggc ggccgatgtc ggcgaaaggc ccgctcatct cggcgagcat
     1621 gcccaccttg atcggctcct gcgcgaaggc cgcgcccgcg gcgagcgcgg cgacgaggac
     1681 gggaacgacg acttcgatcc gcttcatggc tcctcctggg ttgctcgctc aggtcgcggt
     1741 cgccgccgcg gcttcgggga cgggcggcgt ctggggcgcc gtcgccgcgg cctcgagcgc
     1801 cttcgcgccg acgatcagct tcagcacctc gctcgcgcct tcatagatcc gcagcgcgcg
     1861 gatctcgcgg tagaggcgct cgacggtcac gccacgcacg acgccgaggc cgccgaagag
     1921 ctgcagcgcc gagtcgatca cctgctgcgc ggactcggtc gcgtacagct tcgccatcga
     1981 ggcctcgcgc gtcacgcgcg tgccgagcac gtccttggtc caggcggcgc ggtagacgag
     2041 cagggcggag gcgtcgatcg cggtcgccat gtcgccggcc ttcgcctggg tcagctggaa
     2101 ctccgcgagc gtccggccga agaggcggcg atccttcgcg cgggacacgg cctcgtccat
     2161 ggcgcgccgc gcgaagccca gcgccgccgc gccgaccgtc gagcgaaaca cgtcgagcgt
     2221 cgccatcgcg atcctgaatc cctcgcccgg ccggccgagc agccggtcgc ccgcgatgcg
     2281 gcagccgtcg aggcgcacgg tcgcgagcgg gtgcggggcg atcacgtcga tgcgccgcga
     2341 agcgtcgagg ccgtgcgcgt cggcgtcgac gatgaaagcg gagatgcctt tcgcgcccgg
     2401 ggcctcgccc gagcgcgcga acacgacata gtgatcggcg agcccgccgt tcgagatcca
     2461 ggtcttgacg ccgtcgaggc gccagccgtc gccatcgcgc accgcggtgg tcgtcattgc
     2521 ggcgacgtcg gacccggcgt cgaattcgga cagcgcgaac gcggcgatcg cgcgccctga
     2581 tgcgacccgc ggcaggaacg cgcgcttctg cgcctcgctg ccgtggagcg tgatcgggac
     2641 gctgcccagc ccctgcatcg cgagcgcgaa gtcggcgagt ccggaggcgc gcgccagggt
     2701 ctcgcgcgca atgcacaggc tgcggacgtc gaccttgtcg cgcaatccgc cataggcggc
     2761 ggggacgcag gcgcgcagca agccggcctt cccgagctcg gccaccagcc gcccgacgca
     2821 ggcgtagatt gcctcggagc gctcgtcgtc gccgccgacg agcgccggca atgcgtcggc
     2881 cgcccagtgc tcgaactcgc gagcgagcgc gcggtggccg tcgtcgaaga acggccaccc
     2941 caggtgttcc ggcatcgcca tcagttgccc tcgaacacgg gcttctgctt ggcggcgaag
     3001 gcgttgtagg cgcgctcgaa gtcgcgcgtc ttcatgcaga tcgcctgcgc ctgggcctcg
     3061 gcctcgatgc actcgtcgag accgtggctc cattcctggt acagcatggt cttggtcatg
     3121 gcgtggccga aggtcgggcc gtcggcgagg ctcacggcca tcgccgttgc ctcctcgagc
     3181 agcttctcgg gctcgaccag ccggttgtgg aagccgaagc gctcggcctc ctcgccgccc
     3241 acgacccgcc ccgtatacaa gaggtcggcc gcgcgcgaga gcccgacgat gcgcggcagc
     3301 agcgtgcagg cgcccatgtc ggcgccggcg aggccgacgc gcacgaacag gaacgcgagc
     3361 ttggagcgcg cggtggcgta gcgaaggtcg gaggcgcacg cgatcatcgc gcccgcgccc
     3421 gtgcacacgc cgtcgatcgc ggcgacgatg ggctgcgggc aggcgcgcat cgctttcacg
     3481 aggtcgcccg tcatgcgcgt gaacttgagc aggtcgggca tctccatctt caccagcttg
     3541 ccgatgatgt cgtgcacgtc gccgcccgag cagaagttgc cgccggcgcc ggtgatcacg
     3601 accgccttga cgtcgtcggc gtacaccatc cggcggaaca ggtcgcgcag ctcggcgtag
     3661 gactcgagcg tgagcgggtt cttgcgctcg gggcggttga gcgtgatggt cgcgaccctg
     3721 ctcgcgaccg catagctgaa gtgctcggcc tggtagccgc cgagcccctt gttcgtcaca
     3781 cgtccttgca tgtcgttctc ctcagccgac catcgtcaga ccgccgctca ccgacaggac
     3841 ctgaccggtg acgtactcgg cctcgggcgc cgcgaaatac gcgaccgccg cggcgacctc
     3901 ctggggcttg ccgaagcggc gcatcgggat cgccttgatg aaggcctcgc ggtacttctc
     3961 gggcaccgcg gcgagcagcg gggtatcggt cggcccaggg cagacgcagt tcgcggtgac
     4021 gttgtagcgc gcaccctcgc gcgcgaggcc cttggtgaat ccgatcaggc ccgccttcgc
     4081 gcccgagtac acggtctcgc cgaggctccc cacgcggccg gcgtcgctcg agatcgagac
     4141 gatgcggccc gacttgcgct ccatcatctg cggcagcagc gccttggtga ggcgcatggg
     4201 gcccacgaag ttgaggttga tcaccttctc ccagaaagcg tcgtcgcctt cgacgaaggg
     4261 cgatgtcttg ccccagccgg cgccgttcac gaggatgtcg acgcggccga acttcttcag
     4321 cacggcgtcc ttgaaggcgt tgatggaatc ggcgttcgtc aggtcgaggt agaagtactc
     4381 ggccttgcgg ccgttcttgc ggatcgcggc cgcggccgcc tcgccccggt cgcgcgcgac
     4441 gtcgccgatg cagacggtcg cgccttcttc ggcgagcgcc tcggccgttg cctggccgat
     4501 gcccgacgcg ccgccggtca cgacggcgat cttgtcctgg aacttcatga cgtctccttc
     4561 acacggttct cgctcttcga gcggttcaga aaatcggtca ccttgcggcg ggtctcgggg
     4621 tcgccgtaga gcgcgcgcgt gccggcgagc tcctcgacga agccgttgcg ggtgggatgc
     4681 ccgtacgccg agatgcagtg cttcgccgcg gcgagcgcgg cgcgcggcag cgccgcgatg
     4741 ttctgcgcga ccgagcgcgc cttgtcggcg agatcgttgc gccgcgcgca ccattgcacg
     4801 acgcccaggc gctgcgcttc cgcgccgtcg atcacctcgg cgccgaggat cagccgcttc
     4861 gccgcggccg gcccgcacgc gcgcgtcagc cgctgcgtgc cgcccgcgcc ggggaccagg
     4921 ccgagctgcg cctcgggcag tccgagcggg gcgtcggtcg cggcgatccg gaggtcgcag
     4981 gcgagggcga gctcgagccc gccgcccagc gcggcgccgc cgatctcggc gatgctcacc
     5041 accggcgccg cctcgatgcg cgcgtagacg cgctgcatct ggatcacgac ctcgagcatg
     5101 gcgtcggcgc cgtcctcgcg cgcgaagcac gagcgcatca gcgcgaggtc ggcgcccgcg
     5161 cagaaggtct tctggtcgct gcggaagtgc agcaccgaaa agccgtcgcc gaccgcctcg
     5221 tcgagcactt ccccgaggcg ccgcacgagc gcggcgtcga gggcgttcac gggcggccgc
     5281 gacagcgtcg cgacgagcac gcgtcccgcg cgctcgatgg cgatcatggc gccgccttcg
     5341 cgcggtgcgg ctcgaagctg cccgtctcgt ggtagacgac ggcgctgtcc tgctcgaatg
     5401 cgcgcaccga gggcgccgtg cccgggagcc cgagctcctt ccagcgcgcg gcgatgcgct
     5461 cgtaggtgct cttgcggagc gcggtgcgct tcgagaactc gggaaggtga cggtgatcct
     5521 tgcaggcgtt gatcagcgcc ttcgagccgt agggccggtt ctcgggcggg ttctgcgtcg
     5581 gatcgagcca ggtgctccag gtgttgcgca ggatgtcgat gtcgtcgacc ggattgcagc
     5641 ggctcgccat cgcccagatc acctggtcca tgtcggtcgg gtcgatgtcc tcgtcgacgg
     5701 cgatgatcca cttcgtgtag taggcgccgc caggcacctg cgcggcgagc gcgagtacct
     5761 gcgcggcatg cccggcgtgg cgctgctcca ggctcaccac ggtcatgccg aagccgcccg
     5821 cggccgccgg gtgcgagtac acgcccgcga tgcccgggac gccgagcttg tcgaggtcgt
     5881 cccagatctt cgccgagcgg atgatcgaga agaagccgct ctgctcgcac gacgggtagt
     5941 cggccatcag cgcattggtg aggatcgggc tcgagcggta atggaccgcc gtcacctcga
     6001 ccagcggcgc gcccgcctcg gggcgcccgt agtagcccgg gaactcgccg aacggccctt
     6061 cggcgcgcac cgagttcggc cggatgatgc cttcgatcac gagctccgcg tccgcgggca
     6121 ccagcaggtc ggtggtcgtg ccgcgcacca cgcgcacggc ctcgcccttg atgccgcccg
     6181 cgaactcgta ctcggacacg ttcttcggga aggtctgcga gccgaccagc atgaacagcg
     6241 gatcgatgcc ccaggccgcg gcgacctgga tcggctcgcc cttctgccac gcgcgcgtga
     6301 tgtgcagccg cgcgtccttg cccggcgaca gatagagccc gacctcggac ttcccctgca
     6361 gcatcatgcg gtaggtgccg acgttgagat agccgctgtc gggatcgcgc gtgatcaccg
     6421 cgtccgcggt gcccgcgtag cggccaccgt cgagcggcca gtggcgcggg atcggcagcg
     6481 attcgaggtc gatgtcgcgc ccggtgacgg tatgctcgta tacaggcgcg ccgtgcggcg
     6541 acacctcgag cggcgggatg cggcgcttga gcttgtcctt cacgcggcgg atcagctcga
     6601 ccgcgggcgt gtcgaccggc tcctccagcg tgagcgcggt gcggcgccgg ctcgggccga
     6661 tgatgttcca cagcagccgc gcgccgatcg ggctcttctc gaacccgctc gcgcactcga
     6721 acagcacggc gggcgaggga tcctgcttgg cgagcaggta gccgatcgcg ctcatctcct
     6781 cgtcgcggct caccgggtgc tgcacgcgca ccagctcgcc catcgcgtcg acgcgctcga
     6841 gccagtcgcg caggtcgcgg accggcgcgg gccgcgcgcc ggcggccggg gcgagcgacg
     6901 gctgcaggcg gtcgttcacg aggtcacctt cgcgagcact tcgggcgcga cggccggcgc
     6961 cgccggcagg tgggtctccc tgagcttgcg ccgttgcagc ttgccggtgg cggtcaccgg
     7021 cagctcgtcg agccatttca ccagacgcgg ccgcttgatc ttgggcagcg ccgcgatccc
     7081 ggcatcgatt cgcgcgcgcg cctcggcctc gtggccgggt gccgccacgg cgaacagcgc
     7141 gatggccccg aggccgtcgg ccccggtgta gccgaccgcg gcgagctgct gcacggtgcc
     7201 cgcgcacgcg gcgacgagcg cctgctcgat ctcgatcacg ctcacccact ggcccgagat
     7261 cttcagcatg tcgtcgtcgc gtccgcagaa ctcccaggag gtcgagctcg cgggccggaa
     7321 caggtcgccc ggcgagaacc agcccgcctc gaagctgtcg gcctgcgcct gcgggcgctt
     7381 ccagtagccg agcgcgaccg acgggtggcg cacccagatc cgtcccgggg cgccgacgct
     7441 gtggtcgtcg ctgtcgcgcg gccgcaggtc gcagtcgggc gattgcgtga gcagcgtctc
     7501 gtcggcggtg cagtagagga tgaggacgat ggtctcggag gcgccgaagc ccgagaccgg
     7561 cgacacgccg gtcgcctgcc gccacgaatc gcgcagcgac ggcgggaaag cttcgcccgc
     7621 ggacacgaaa tgccgcacgc cggccgcgga gagccccggc gcgagcccgg cctgcaggag
     7681 cttggagtac agggtcggca ccgagaacat gaccgtgggc gcgtagcgct cgacgatgcg
     7741 cgcggtgcgc tccgcggtgg gccactcggg gtcgagaatc acggtcgcgc cgagccgcag
     7801 cccggcgaac agcgcgttgc cgagcgcgta agcgaagaac agcttcgacg tcgcgtacag
     7861 gcgcgcgtcg gagccgacgc cgagaatcgt gctcgcgaag ttggcggtct cgacgaccgc
     7921 acgctgcgcg tgcatcacgc ccttcgggac gccggtggtg cccgaggtgt agatccagaa
     7981 ggcgggcgag tcgtcggaaa gctgctcggg cgagcgcacg ggcacggcgt cgcggatcgc
     8041 gcgcgcctcg gcgatggtga cgatgcgcgg gctgctgcct tcggcgcgcc cgaggcgcgc
     8101 gagcgtcttc agcccggcct cgtcgcagac gatgaagcgc gcgccgctgt cgtcgagcac
     8161 gacgcccagg tccttgtcga agaggcgcga gttcaggccg acggcgacgc cgcccgccca
     8221 catggcgccc aggtaggcga tgacccagtc gatgctgtcg ggcgcgaaga tcagcacccg
     8281 gtccccggtc tcgaggccga gacggcgcca gccggcggcc gcgcgcgcca ccgcgttcct
     8341 cagatcccga taggaaacgc gcgcgttgcc gcactcgagc gcggtgcgcg cgtcgtctcc
     8401 cgccgaaagc agagactccg ctgcgttcat cggcatgtga ctcctcctgg cgccccggtc
     8461 cgtggagtcc ggccgcggtt ctacgggacg tggacgattt ctataacgga ccggcccggc
     8521 gcctttgacc caagtcaatc gggcccgaga taggtcccga aatgacgcgg atcgcgcgcg
     8581 tccgtagaat gacgcgcagg ccaatcggag gaggaccgcc atgaagccgt cgccgctgcg
     8641 agtcgtgggg ctgggactga tcgcgttgat cctcgccggg gcggcggcgg cgcaagcgcc
     8701 cgcggcctat ccgtcgcggc cgatcaagat catcgtgccg ttcccgcccg gcaacaccat
     8761 ggacatcatg tcgcgcctca tcggcccgaa gatgagcgag cggctcggcc agcagatcct
     8821 ggtcgagaac cgtcccggcg cgagcgggat gctggggctg gatctcgtcg ccaaggcgcc
     8881 cgccgacggc tacacggtcg gcgccggcca gggcggtaac atggtggtgc tgccgcacac
     8941 gagccggaac attccctacg acccgctcaa ggacttcgcg cccatcgcgg tgtcgacgac
     9001 caattacctc gcgatcgtcg ccaacccgca ggcgccgttc cgggacatcg ccggcatgat
     9061 tgcgtacgcg aaggcgaacc cgggcaagct caccgtcgcg acgaacggcg agggcggctt
     9121 cccgcacctc gcgttcgagc acctgcgcct tctcggcggc ttcacgttca cgcacgtgcc
     9181 gtacacggga tcggccgcga tcgcgaccga cgtcatcggc ggccaggtgc aggtcgggat
     9241 cgacggcatc acggggctca cgccgcacgt gaagtcgggg cgcctgcggc tgctcgcgat
     9301 caccaaccgg aagcgcgtcc cgctctggcc cgacacgcag gccgcggccg aggcggtgcc
     9361 gggctacgag tcgggcggct ggttcggcta cgtcgcgccc gcggcgaccc cgcgcgcgat
     9421 catcgccagg ctgaacgccg agatcaaccg cgcgatgcag ctgcccgacg tgagcgacaa
     9481 gctcgtgagc gccggcctca tcgtcgtcgc cgagtcgccc gagtacttcg cgaacgtgat
     9541 ccgcagcgac tacgccaagt acggcaagct cgtccgcgac atcggcttca cgccgcagtg
     9601 acgccgggcg tcactggatc ttgaggccgg cggccttcac caccttcccg gtttcgcgga
     9661 tttcgtcctc gacgagcttg cggaactcct cgggcgtgtc ggtcatgatc tcggcgccct
     9721 gcttctcgat cgcggccttc acctcgggca gctccagcgt cttcaccagc gccgcgcgca
     9781 gcttgtcgat caccgggcga ggcgtgccct tcggcgtgac caggccgttc caggcgctgt
     9841 agctgaagcc ggggatggtc tcggccaccg gcggcagacc cgggagcagc ggcgtgcgct
     9901 gcggcaggct ctgcgcgagc gcgcgcagct tgccgctcct cacgtggccg attgcggccg
     9961 acgcgggcgt gatcgtccac tgcgactcgc ccgtcatgac cgagagcacc gacgcgccgc
    10021 cgcctttgta cggcacgtgc acggactcga agtggcccat cacctgcatc atcgcgccgg
    10081 cgaggtggct ctgcgagccc gggcccgcgg aggccatgtt gacctggccg ggatgcgact
    10141 tgcaccagtc gatcagctcc ttcgtgctct tcaccggcag cgacggcgtc accacgagca
    10201 tgttcgggag caccgcgaac atcgagacga actcgaagtc cttgagcggg tcgtacggca
    10261 ggctcttcct gaggttcggc gcgatggtca tgcccgaggg cgacgccgac gcgatggtgt
    10321 agccgtcggg cgccgagttc cgggtcgact ccatgccgat caggccgctc gcgcccgcct
    10381 ggttctcgat cacgatctgc tggccgatga cgtcccccat gcgcgcgccg agcacgcgcg
    10441 agagcgtgtc gttggagctg cccggcgcgt tgggcaggat catcttgatc gggcggcgcg
    10501 ggtagtcgct ctgcgcgagc gccgtctgcg cgacgagcgc gagcagcagg agtcggacgc
    10561 acttcgatgc gagtctcatt tcgggtctcc tctcaaaaga cgcgtcagta gacgcgatga
    10621 tgcggcggtt cgcccttgac gagcacccgc tcgacgttgg cccaggcgct ccggaacagg
    10681 agcgccgagg attgacgcgt gacgccggcg acgtgcggcg tcagcagcag ccttctcgcc
    10741 gcatcgcccc ggagcgcgag cagcgggtga tcgggagcgg gcggctcgct cgagtagacg
    10801 tcgaccgcgg cgccgccgag gcgccccgac tcgagcgcgc gcgcgagcgc ggcctcgtcc
    10861 accacgccgc cgcgcgaggc ctggaccaga acggcgcccg gcttcatcgc ggcgagctcg
    10921 cgctcgccga tcagcccggt ggtctcgggc agcaacggga cgtgaacgct gacgacgtcc
    10981 gagaccgcga gcagctcgtc gaggccgacg cggcgcgcgc ccggcgcctc gcgcggcgcg
    11041 ggatcgaagt agacgatcgt gcacccggag cgctcgaacg cccttgcgac cgccgtcccg
    11101 atcgtgccga ggccgatcag ccccacggta aggccctcga tgcccgagag gttgtcggcg
    11161 agcatgcgcg cgcggaaggc ggcgtagttg ccggcccgga cttcggcgtc ggcccacgcg
    11221 aagcgccgca gcagcagcga cgccgccgtg accgcgtact cggcgagcgc ggcgttgctg
    11281 ccgcccggga cgttggccac ggggatcccg aggcgcttca tcgccgccgc gtcgaggcgg
    11341 tcgaagcccg cgcccgtcac ctgcaccagg gcgaggcgcg caccctcgaa cagcggcgcc
    11401 gccagcttcg cgcccaaggc tgggatcacg agcgcgcgcg agcgcgcgat catgacggag
    11461 agctgcgggt cgtcgggcct ggcgtacgag acggcgagcg acgcgggggg aagcgcgtcg
    11521 acgcgctcga agtcggcgcg cggccgaagg cagaggacgg cgtgcgtcac ttcccgaggt
    11581 cgcggttgac gacgcgcgca gtcggaaaga agcagagcag cacgagcggc tcgccgcccg
    11641 tgttgcgggt cacgtgcgtc tcgcccgcgg gaatcaggat cgtgtcgccg gcgcgcagct
    11701 cgtggtctcc gctgtcggat tcggtgcgcc cctgtcccga gagcacgtag atgcactcct
    11761 cggtgtcggc gtggaaatgc cgtccccgcg gcttgtcacc cggctgcggc actgcgatct
    11821 cgacgcgccg cagcgtcacg gcgtgcgagc cgctcgcgcc cgacacgatc tcgagcgagc
    11881 ggcgcccggg gagtccgagc tcgcgcgcct gtgcgggctt gacgacgcgc gccatcagag
    11941 cgcgaccttg aggtgcttga cctcggccgt gccgccctgc gcctcgtcga gcatgcggaa
    12001 aagctcccgg gcttccttcg cgatggcatc ggcgatgtcg gcgagcatct tcacgccctt
    12061 gtcgacggtg gcgtgctcgg gcgcgccgta ccagcccgtc ggcgcgatgg tgcggatgtc
    12121 gcgcagcacc ggctggaagc tgcgcgtgcg ccgcagcttg tcccacttgc ggccgtgcga
    12181 atggtccgac gaatagtcga tgcggtcgag gtgcacgagg tcgggccggc aggcgagcac
    12241 cgcgagcgcc tcgatctcgc cgccgtgggt gagcccgccg cagtccttgc cgtagagatc
    12301 ggccgcgacg tagcaagcct gcacggtcag caccgtcatg ccgtgctcgc ggtgcagcga
    12361 ctcggccgcg atcgcgagcg acgggatgtt gccctcgtgc cagttcagga tgaggagata
    12421 cttcgcgccg tgcttcgccg tggccgcgca ggtctcgacg accacgcgca tgtaggtctc
    12481 gggcgtcagc gtgatcgttc cctcgaacgg catgtgcatc ggcgtcacgc cgagcgggcc
    12541 gccggggagc accacgccgt ccatgcgctc ggccaccgcg tggccgatca cgttggaggc
    12601 gaagatgtcg gtgcccgtcg gcagatgcgg gccgtgctgc tcgacgctgc ccgccggcag
    12661 gatcacgagc ggattgcggc ggaactgcgc ggcgatctcg ggctgggtca gctcctggaa
    12721 gtagcggctg ggcggattca tgccggttcc tttttcaggc ggcgcgctgc gcgcggcccg
    12781 cgatcctggc gcgcgtctcg tcgacgacct cggccagcgt gaccgcctgg cccctgcgct
    12841 cgatcgagcg cagcgccgcg tagaccgcgg cgagcgcgac gttgccgttg tgcgccgtga
    12901 gctcgcccga gcgcccggtg cggcagctct cggcgaacat ctcgagctcg gtgcggaaca
    12961 tgtcgctctc ggggaccgcg aggacctcgc gccgggcgta gccgtccttg ccgcgctgga
    13021 tgtagagcgt cgcgctcttg tgcaggagat gcggcgtgtc ccaggtgccg aagtcgatct
    13081 cgtagtgcat caggcccttc gagctgaaca cgcgcagcgc gaacacgcct ggggaggtcc
    13141 agcacgagcc gacgtagccg atcttgccgt cggcgaagcg caccgtcgtc atcgactggt
    13201 cgtcgacctc ggcgccgacg ggcgagagct tggacgcggt cgagctcacc tcggtcatct
    13261 cgccgccgag gtagtgcagc acgtcgaagt ggtggatggc gagctgcgac agcgggccgc
    13321 cgggcgcctt gtccttgtac cagcgccagg tcttgggcgt cagctcgagc gcgcgctcgt
    13381 tggagaagtt cgcctcgatg acggcgacgc ggaccagctc gcccgactcg atcttcgcgc
    13441 ggatcgcgcg gatcccggcc atcaggcgcg cgctgtggcc gactgtcacg gtgatgccgt
    13501 gctcgcgctc gaggtccgcc atctgcagac cgtcctcgag cgtgttggcg atcggcttct
    13561 ccgtatacac gtgcttgccg gcggcggcga cctggcgcgc gagcggccag tgctgctcgt
    13621 tggggacggt gaggatcacg ccccggatcc ggtcgtcggc gagcagggcc ttgaggtcgg
    13681 cgacgggggc gacgccgaac tcggcggcga atgccgcgcg cttgtcctcc gagcggctgg
    13741 tcgcggagac gatcctgatc ttgtccgatt gcgcggcggc gcgggcgagc accttggccc
    13801 agcggccgag accgacgatg ccgacgttga cgggagaatc catgaatggt cctgcgaggc
    13861 gcgagaacgg aactggcgct attgtaatac ggagaccacg aagcgaggat caggcatgga
    13921 ccctctcgag accctgcgcc acgacgtcgc gctcgccaac cgcgtgatcg agcgcttcgg
    13981 cctcgcgaac gcgttcggcc acgtgagcgc gcgcatcccc ggcacgagca cgttctactt
    14041 cccgacgcgg cgctcgcccg gcctcgccga cgagaagcgg ctgctcgtcc tggacaccga
    14101 cggcaacatc gtctcgggcg acggcatccc gaacagcgag ctgtggatcc acgcgcgcat
    14161 ctacgccgcg cggcccgacg tcggcggcgt cgcgcacgcg cacccgggcg cgtgcgtgtg
    14221 cctgacgctg atcgggcagc cgctgcgcgt cgtgcacaac atgggcggcg cct
//
LOCUS       DATDMW010000406         6134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1117521, whole genome shotgun sequence.
ACCESSION   DATDMW010000406 DATDMW010000000
VERSION     DATDMW010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117521"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VI319_16865"
     CDS             complement(<1..608)
                     /locus_tag="VI319_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865566.1"
                     /translation="MNKPVLAALVAAGILSACGGGGGGSGGTRPADPVPVATSSSSDQ
                     GPNPQLAPLGQVAAVDHHIDFTQQTALTPAGLGSFDSLSDMPAPEVSLANTPLIHVAI
                     PDLGIDDVFDGPVTTTMVNGLRADTSSKSDATTRRTVTYLVPDAASTNPLKLQFSSLG
                     VWNTTDIASGQMTNAAAFSVGTRTLGTDIPTNGTATYNGAMLG"
     gene            complement(730..2052)
                     /locus_tag="VI319_16870"
     CDS             complement(730..2052)
                     /locus_tag="VI319_16870"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6865567.1"
                     /translation="MTGNSLLDEALRAQAAGRAREAVELYGHVLRYDPDQLVALNNLG
                     LLLGEAQRWSESEALLARAARVAPRRADAWVNLATVLAGQKKLDDAAAACRRALEIAP
                     ENRQAQNTLASVLADSGRHDEAIALLERIVRKRPGYATGHAFLGSLYAKRARHEDAVA
                     SFRRAFDADPKDRVSLAAAAECLLVLGRAAEALPLLERALALDVYDVRALALKTLALA
                     ETGRGDEERALSDPGRLIHGMTLAGLGYAPGEVADLNRGLAEFAAAEAGMQQDPAQYA
                     TYRGWHSTTNLAHAPHPAIAKLKEAIRRAFDERLRRLPEEDASHPLARAAPRDYALDL
                     WCVRLSGGGGKMLPHIHLAGWLSGVYYVEVPKIVDDPAAGRAGWIEFGPPRADIRLTR
                     SPLVRAVKPEPGLLLTFPSYFWHDTVPLPESTVGQRLIFSWDYQPLRG"
     gene            complement(2049..3977)
                     /locus_tag="VI319_16875"
     CDS             complement(2049..3977)
                     /locus_tag="VI319_16875"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY6865568.1"
                     /translation="MRELVARFLSRSISRRGFLKGLTATGVSLAAAESILESVSSVAY
                     AQGEGSSASLSPDAVKVVEGTGGECFAEQLVASGVKYIFGNSASEDAHFYEALVDRPQ
                     LKYILTPHEGPGAAMAAGYVKASGEPAIVMQAAAVGLMNAMGQMFNAFKEQTPLVFYS
                     YRTDQTGRAGRDGFEEVANQEQLVQPITKYSWLARRADMIPETVRRAFKAAWTPPHGP
                     TYASWHSDLNDQRTHAEVIVQDKFDPRMRVRPNPQEIERAAKLLVEAKMPLMIVGDEI
                     YNARAIGKAVRLAEILGMPVTQARQIFVSFPETHPLWVGALPVTRIASLDFPKNPDVV
                     INVGNKLQHNSPAPIVGRGPKFIDMRIDSWSMGNVIPTDVPLVCDVALGLDDLIAAVR
                     QLVNADAGRRAQERADQVRAFSERARTLRNGVTKNPDWNRAPMIADRVTWEVAQFADR
                     DAIVVNEAGSVNLHSFDFNAEGGRELFHYYGAHLGSGVGTAAGVKLARPSRQVICMVG
                     DGSFIFGPTALWNMARLELPVITVVYNNHAYSGPHSRVIEKVPGGRMIQTGHFVHDYL
                     GNPDMNMAYIARGFGVQAEVANNPAELRAALARAKKATQEGKPYLIDAQVARVGVAWA
                     DKPWTPSVRETQGRARKS"
     gene            complement(3987..4349)
                     /locus_tag="VI319_16880"
     CDS             complement(3987..4349)
                     /locus_tag="VI319_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865569.1"
                     /translation="MRFFPAIIAVLAAVIVTGAAPAMAQQRPSTGSGRSAANAFPDAP
                     GKEVLVAKCFQCHSPNMWSDLHQDRRAWEATLYRMVGRGALWSEDEIKKMADYLGATH
                     AREPSVFGREPVQSPAAQ"
     gene            4436..>6134
                     /locus_tag="VI319_16885"
     CDS             4436..>6134
                     /locus_tag="VI319_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865570.1"
                     /translation="MAGFARIAVAFALLVAALAARAQFVDPGLRWRTFDTEHFSVHFP
                     DRNRAQADVAAQVAESVYPRITRFLQWQPQSRVHLLVLDSADFSNGWSTPLPFNEFAI
                     YLSPPDEGELLQNRGWMEMVISHELTHIVHMDKARRGPLVLRNVFGRVPLPLPNTLPN
                     LWGPSWVKEGLAVYSESDAAGGYGRLGQSFFEGMMRAEVDRGLRSLREIHADGRGFPL
                     NRDYLYGSYFYMFLAERYGPEAVFQYIDNYSDDLLPFRMHSNPYPATGKPMDVLWVEY
                     LDWLDARFRAPRPAPDEGEALLQAFSIASPAIAPDGTRWYVRHDGYTRPKLVEQRPGG
                     EPRVVREVEPGTRLSIDAAGRPLLAQPEICGNYNYYYDLWRGGTGVFGGFERLTHCSR
                     LRFAAPLADGRIVALRIAGGEGQVVLVGADGAEQSTLYRATPGEMITGVAARGPAVVL
                     TSWRGGRWTLLEVRDGRATELVADSAVKHSPRFGDSDDEVFFVADYGKVYNVWSYSLS
                     SRRLARWTQAPYGVQDISAPVGGEMLLTTIEANGDILRARRMPQAPLERREATVAAAT
                     "
BASE COUNT          818 a         2148 c         2215 g          953 t
ORIGIN      
        1 ccgagcatcg cgccgttgta ggtcgcggtg ccgttggtcg ggatgtcggt gccgagcgtg
       61 cgcgtgccga ccgagaaggc ggccgcgttc gtcatctgcc cgctcgcgat gtcggtcgtg
      121 ttccagacgc cgaggctcga gaactgcagc ttgagcggat tggtgctcgc cgcgtcgggc
      181 acgaggtagg tgacggtgcg gcgcgtggtg gcgtccgact tcgaggaggt gtcggcgcgc
      241 aggccgttca ccatcgtcgt cgtcaccggg ccgtcgaaca cgtcgtcgat gccgagatcg
      301 gggatcgcga cgtggatcag cggcgtgttc gcgagcgaca cctcgggcgc gggcatgtcg
      361 ctcagcgagt cgaatgagcc gaggcccgcg ggcgtgagcg cggtctgctg ggtgaaatcg
      421 atgtggtgat cgacggcggc gacctggccg agcggcgcga gctgcgggtt ggggccctga
      481 tcgctggagc tgctggtcgc gacgggcacc gggtccgcgg gccgagttcc gcccgagcct
      541 ccgcctccgc cgccgcatgc gctgagaatc cctgcggcga cgagcgccgc gagaaccggc
      601 ttgttcattg tgcttctccc ttacgaatgg gttcttgatg acccgggcgg attcttgccg
      661 ggaaagcgac ttcgcgaaat agcccggacg gcgcgtctct cgccggagac ggttcagcga
      721 accggctggc taaccccgaa ggggttggta atcccaggag aagatcaggc gctggccgac
      781 ggtgctctcg ggcagcggca cggtgtcgtg ccagaagtaa gacggaaacg tgagcagcag
      841 cccgggctcg ggcttgaccg cgcgcacgag cggcgagcgc gtcaggcgga tgtcggcgcg
      901 cggcgggccg aactcgatcc agccggcgcg gcccgcggcc gggtcgtcga cgatcttcgg
      961 gacctccacg taatagacgc ccgagagcca tcccgcgaga tggatgtgcg gcagcatctt
     1021 gccgcctccg cccgagaggc gcacgcacca gagatcgagc gcgtagtctc gcggcgcggc
     1081 gcgcgcgagc gggtgcgacg cgtcctcctc gggcagacgc cgcaggcgct cgtcgaaggc
     1141 gcggcggatc gcctccttca gcttcgcgat cgcggggtgc ggcgcgtgcg cgaggttggt
     1201 cgtgctgtgc cagccgcggt aggtcgcgta ctgcgccggg tcctgctgca tcccggcctc
     1261 ggccgccgcg aactcggcga gcccgcggtt gagatcggcg acctcgccgg gggcgtagcc
     1321 gagcccggcg agcgtcatgc cgtggatcag ccgcccgggg tcggagagcg cgcgctcctc
     1381 gtcgccgcgc ccggtctcgg cgagcgcgag cgtcttcagc gcgagcgcgc gcacgtcgta
     1441 gacgtcgagc gcgagcgcgc gctcgaggag cggcagcgcc tcggctgccc gcccgagcac
     1501 gagcaggcac tcggccgcgg ccgcgagcga aacccggtcc ttcggatccg cgtcgaacgc
     1561 gcgccggaac gaggcgaccg cgtcctcgtg ccgcgcgcgc ttcgcgtaca ggctgccgag
     1621 gaaggcgtgg ccggtcgcgt atcccgggcg cttcctgacg atccgctcga gcagcgcgat
     1681 cgcctcgtcg tggcggcccg agtcggcgag caccgaggcg agcgtgttct gtgcctggcg
     1741 gttctcgggc gcgatctcga gcgcccggcg gcacgcggcc gcggcgtcgt cgagcttttt
     1801 ctggccggcg agcacggtcg cgaggttgac ccaggcgtcg gcacggcgcg gcgcgacccg
     1861 cgcggcgcgc gcgagcagcg cctcggactc gctccagcgc tgcgcctcgc cgagcagcag
     1921 cccgaggttg ttgagcgcga ccagctggtc ggggtcgtag cgcaggacgt ggccgtagag
     1981 ctcgaccgcc tcgcgcgcgc gcccggccgc ctgcgcgcgc agcgcctcgt cgagcagcga
     2041 gttgcccgtc acgacttgcg tgcgcgcccc tgcgtctcgc gcacgctcgg cgtccagggc
     2101 ttgtcggccc aggcgacgcc gacgcgcgcg acttgcgcgt cgatcaggta gggcttgcct
     2161 tcctgcgtcg ctttcttcgc gcgcgcgagc gcggcgcgca gctcggccgg gttgttcgcg
     2221 acctcggcct gcacgccgaa gccgcgcgcg atgtacgcca tgttcatgtc cgggttgccg
     2281 aggtagtcgt gcacgaagtg cccggtctgg atcatgcgcc cgcccggcac cttctcgatc
     2341 acgcggctgt gcgggccgga gtaggcgtgg ttgttgtaga cgacggtgat caccggcagc
     2401 tcgagccgcg ccatgttcca gagcgcggtc ggcccgaaga tgaacgagcc gtcgccgacc
     2461 atgcagatca cctggcggct cgggcgcgcg agcttcacgc ccgccgccgt gcccacgccc
     2521 gatccgaggt gcgcgccgta atagtggaag agctcccgcc cgccctcggc gttgaagtcg
     2581 aagctgtgca ggttcaccga gcccgcctcg ttgacgacga tcgcgtcgcg gtcggcgaac
     2641 tgcgccacct cccaggtcac gcggtcggcg atcatgggcg cgcggttcca gtcggggttc
     2701 ttcgtgacgc cgttgcgcag cgtgcgcgcg cgctcgctga aggcgcgcac ctggtcggcg
     2761 cgctcctgcg cgcgcctgcc cgcgtcggcg ttcaccagct ggcgcaccgc ggcgatcagg
     2821 tcgtcgaggc cgagcgcgac gtcgcacacc agggggacgt cggtcgggat gacgttgccc
     2881 atgctccagc tgtcgatgcg catgtcgatg aacttcggcc cgcggccgac gatgggcgcc
     2941 gggctgttgt gctggagctt gttgccgacg ttgatgacga cgtcgggatt cttcgggaag
     3001 tcgagcgacg cgatgcgcgt caccggcagc gcgcccaccc acagcgggtg ggtctccggg
     3061 aagctgacga agatctggcg cgcctgcgtc accggcatgc cgaggatctc ggcgaggcgc
     3121 accgccttgc cgatcgcgcg cgcgttgtag atctcgtcgc cgacgatcat gagcggcatc
     3181 ttcgcctcga ccagcagctt cgccgcccgc tcgatctcct gcgggttcgg gcgcacgcgc
     3241 atgcgcgggt cgaacttgtc ctggacgatg acctcggcgt gcgtgcgctg gtcgttcagg
     3301 tccgagtgcc acgacgcgta ggtcggcccg tgcggcgggg tccacgccgc cttgaaggcg
     3361 cggcgcaccg tctccgggat catgtcggcg cggcgcgcga gccacgagta cttcgtgatc
     3421 ggctggacca gctgctcctg gttcgcgacc tcctcgaagc cgtcgcgccc ggcgcgcccg
     3481 gtctggtcgg tgcggtagct gtagaagacg agcggcgtct gctccttgaa cgcgttgaac
     3541 atctggccca tcgcgttcat gagcccgacg gccgccgcct gcatcacgat cgcgggctcg
     3601 cccgaggcct tgacgtagcc cgcggccatc gccgcgccgg ggccttcgtg cggcgtcagg
     3661 atgtacttga gctgcggccg gtcgaccagc gcctcgtaga agtgcgcgtc ctcgctcgcc
     3721 gagttgccga aaatgtattt cacgcccgag gcgaccagct gctcggcgaa gcattcgccg
     3781 ccggtgccct ccacgacctt gaccgcgtcg ggcgacaggg acgcggacga gccttcgccc
     3841 tgcgcgtacg cgaccgacga caccgactcg aggatcgact cggccgcggc gagcgagacg
     3901 cccgtggcgg tgagcccctt caggaagccg cggcgcgaga tcgaccgcga aagaaatcgt
     3961 gcgacgagct ctctcatggc ctctccttat tgcgccgcgg gcgactgcac cggctcgcgc
     4021 ccgaacaccg acggctcgcg cgcgtgcgtc gcgccgaggt agtcggccat cttcttgatc
     4081 tcgtcctcgc tccagagcgc gccgcgtccc accatgcggt acagcgtcgc ctcccaggcg
     4141 cggcggtcct ggtggaggtc ggaccacatg ttcgggctgt ggcactggaa gcacttggcg
     4201 accagcacct ccttgcccgg ggcgtcgggg aatgcgttgg ccgccgatcg tcctgagcct
     4261 gtcgaaggac gttgctgggc catcgcgggc gcggcgcccg ttacgatgac cgcggccagc
     4321 acggcgatga tcgcggggaa gaatctcatc cgggcgcctc ctctgcggaa ccggccataa
     4381 atatacggca atggtctatg atcgtgccgg cattataaga aagccgggga gaagcatggc
     4441 tggtttcgcg cgcatcgccg tggcgttcgc tctgctcgtc gcggcgctcg cggcgcgggc
     4501 gcagttcgtg gacccgggcc tgcgctggag gaccttcgac accgagcact tctcggtgca
     4561 tttccccgac cgcaaccgcg cgcaggccga cgtcgccgcg caggtcgccg agagcgtgta
     4621 cccgcgcatc acccggttcc tgcagtggca gccgcagtcg cgcgtgcacc tgctcgtcct
     4681 cgactcggcc gatttctcga acggctggtc gacgccgctc ccgttcaacg agttcgcgat
     4741 ctatctctcg ccgcccgacg agggcgagct gctgcagaac cgcggctgga tggagatggt
     4801 gatctcgcac gagctcacgc acatcgtgca catggacaag gcgcggcgcg gcccgctcgt
     4861 gctgcgcaac gtcttcggcc gcgtgccgct gccgctgccg aacacgctgc cgaacctgtg
     4921 ggggccttcg tgggtgaagg aggggctcgc ggtgtactcg gaatcggacg ccgccggcgg
     4981 ctacgggcgc ctcgggcaga gcttcttcga gggcatgatg cgcgccgagg tcgatcgcgg
     5041 cctgcgctcc ctgcgcgaga tccacgccga cggccgcggc ttcccgctca accgcgacta
     5101 cctctacggc agctacttct acatgttcct cgccgagcgc tacgggccgg aggcggtgtt
     5161 ccagtacatc gataactaca gcgacgacct gctgccgttc cgcatgcaca gcaatcccta
     5221 cccggcgacg ggcaagccga tggacgtgct ctgggtcgag tacctggact ggctcgacgc
     5281 gcgcttccgc gcgccgcgcc ccgcgcccga cgaaggcgag gcgctgctgc aggccttctc
     5341 gatcgcgagc cccgcgatcg cgcccgacgg cacgcgctgg tacgtgcgcc acgacggcta
     5401 cacgcgcccg aagctcgtcg agcagcgccc gggcggcgag ccgcgcgtcg tgcgcgaggt
     5461 cgagcccggc acgcgcctct cgatcgacgc cgcgggccgc ccgctgctcg cgcagcccga
     5521 gatctgcggc aactacaact actactacga cctctggcgc ggcggcacgg gcgtcttcgg
     5581 cggcttcgag cgtctgacgc attgctcgcg cctgcgcttc gccgcgccgc tcgccgacgg
     5641 ccgcatcgtc gcgctgcgca tcgcgggcgg cgaagggcag gtcgtgctcg tcggcgcgga
     5701 cggcgccgag cagtcgacgc tctaccgcgc gacgccgggc gagatgatca cgggcgtcgc
     5761 cgcgcgcggc ccggccgtgg tcctcacgag ctggcgcggc gggcgctgga cgctgctcga
     5821 ggtgcgcgac ggccgcgcga ccgagctcgt cgccgactcc gcggtcaagc actcgccgcg
     5881 cttcggcgac tccgacgacg aggtgttctt cgtcgccgac tacggcaagg tctacaacgt
     5941 ctggtcgtac tcgctttcgt cgcgccggct cgcgcgctgg acccaggcgc cttacggcgt
     6001 gcaggacatc agcgcgccgg tcggcggcga gatgctgctg acgaccatcg aggcgaacgg
     6061 cgacatcctg cgggcgcgcc ggatgccgca ggcgccgctc gagcgcaggg aagcgaccgt
     6121 cgccgccgcc accg
//
LOCUS       DATDMW010000407         3165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1241707, whole genome shotgun sequence.
ACCESSION   DATDMW010000407 DATDMW010000000
VERSION     DATDMW010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241707"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VI319_16890"
     CDS             complement(<1..852)
                     /locus_tag="VI319_16890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6865571.1"
                     /translation="MKNRITGAALAALLLAAIAFPAQAQMGSKVLHIVVPFAAGGARE
                     ILARTFYSELGAALGQTAIIENRPGAGGAIGTSSVAKAAPDGQTLIFAASSHNVTALL
                     GANPPYDPVRDFAPVANIGMQSYVLMASATLPARTVAELVRYAKANPGKLNYASPGHG
                     SSGHLAAAYFASLAGLDMVHIPFKSTQDATNDVLAGRSHLVIVPNVGAVPFMKDARIR
                     LLGVTSLKPSPSIPGVPPIANTVRGYQFDSWFGLLAPARTPKPVIDRINAEVAKLLKN
                     PTILERLS"
     gene            complement(849..1871)
                     /locus_tag="VI319_16895"
     CDS             complement(849..1871)
                     /locus_tag="VI319_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6865572.1"
                     /translation="MIIDCHGHYTTAPKDLQKWRDAQIAALKDAKHPPTSKGSLAISD
                     DQIRESLENAQLRIQRERGTDVTIFSPRAAGMGHHIGDETTSRHWSEHCNELIHRVCA
                     LYPQNFVGVCQLPQTPGVSPKNCIAELERCVRDFGFIGCNLNPDPSGGYWTDPPLTDK
                     FWYPIYEKMVELEVPAMIHVSTSCNPNFHTTGGHYINGDTTAFMQLISAPQIFKDFPT
                     LKFVIPHGGGAVPYHWGRYRGLAQDMGRPPLKDLLLDNVFFDTCVYHRPGIELLLKVI
                     PADNILFASEMVGAVKGIDPETGHYYDDTKRYLDAIDINPADREKIFSGNVSKVYPRF
                     ATKAKK"
     gene            1946..2911
                     /locus_tag="VI319_16900"
     CDS             1946..2911
                     /locus_tag="VI319_16900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6865573.1"
                     /translation="MCSARLVAFAALLACGVASAQTTDAYPTRPIRYLVASAPGGIAD
                     ITARLLGPHLSEALRQPVVIENRPGSILVGVELVAKAPADGYTLLSATPQVAIAQSMY
                     KRARVNPRAELAPVALVGMIPNVLVVGPRTKAKSVAELVELARANPGKLNYSSTGAGT
                     SVHLAAELLKYMANVDIVHVPYHGAAAAMTALIAGDVDMLVDSLPPSLPHIRAGRVRA
                     LAVTSATRAPQLPDVPTMAEAGFPGFEVSAWSGVVAAAATPPEIVARLESVIGRALED
                     PRAAATFEKAGLPVRFLGAKDFGAFWDREIDKFALAVKHSGAAVE"
     gene            2921..>3165
                     /locus_tag="VI319_16905"
     CDS             2921..>3165
                     /locus_tag="VI319_16905"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865574.1"
                     /translation="MSAIERREIEYAVHGGAALKGHLYAPRGGGRVPAVIAVHGGGWR
                     LANLDNYAHLGPWLAERGYAVFAVTHRLSSASTRVYP"
BASE COUNT          445 a         1043 c         1151 g          526 t
ORIGIN      
        1 cgagaggcgc tcgaggatcg tcgggttctt cagcagcttc gcgacctcgg cgttgatgcg
       61 atcgatcacg ggcttcggcg tgcgcgccgg cgcgagcagg ccgaaccagg agtcgaactg
      121 gtagccccgc accgtgttcg cgatcggcgg cacaccgggg atcgaaggcg acggcttgag
      181 cgacgtcacc ccgagcagcc ggatgcgcgc gtccttcatg aacggcaccg cgccgacgtt
      241 gggcacgatg acgaggtgcg agcgccccgc gagcacgtcg ttggtcgcgt cctgcgtcga
      301 cttgaacggg atgtggacca tgtcgaggcc cgcgaggctc gcgaagtagg cggccgcgag
      361 gtgcccggag ctgccgtggc cgggcgaggc gtagttgagc ttgccgggat tggctttcgc
      421 gtagcgcacc agctcggcga cggtgcgcgc cgggagggtc gcgctcgcca tcagcacgta
      481 gctctgcatc ccgatgttcg ccacgggcgc gaaatcacgg acgggatcgt agggcgggtt
      541 cgcgccgagc agcgcggtga cgttgtggct cgacgcggcg aagatcagcg tctggccgtc
      601 gggcgcggcc ttcgccacgc tcgaggtgcc gatcgcgccg cccgcgccgg gcctgttctc
      661 gatgatcgcc gtctgcccga gcgccgcgcc cagctcgctg tagaaggtcc gcgccaggat
      721 ctcgcgcgcg ccgcccgcgg cgaaggggac gacgatgtgc aggaccttgc tgcccatctg
      781 cgcctgcgcg gggaaggcga tcgccgccag gagcagcgcg gcgagcgccg cgccggtgat
      841 gcgattcttc atttcttggc tttcgtggcg aagcgcggat agaccttgct cacgttcccc
      901 gagaagatct tctcgcggtc ggcagggttg atgtcgatcg cgtcgagata gcgcttggtg
      961 tcgtcgtaat aatgtccggt ctcggggtcg atgcccttca ccgcgccgac catctccgag
     1021 gcaaacagga tgttgtccgc cgggatcacc ttgagcagca gctcgatccc cggccggtga
     1081 tacacgcagg tgtcgaagaa gacgttgtcg agcagcaggt ccttcagcgg cggccggccc
     1141 atgtcctgcg cgagcccccg gtagcgtccc cagtggtaag gcaccgcgcc gccgccgtgc
     1201 gggatgacga acttgagcgt ggggaaatcc ttgaagatct gcggcgcgga gatgagctgc
     1261 atgaacgcgg tcgtgtcgcc gttgatgtag tggccgcccg tggtgtggaa gttggggttg
     1321 cacgaggtcg acacgtggat catcgccggc acctcgagct ccaccatctt ctcgtagatc
     1381 gggtaccaga acttgtcggt gagcggcggg tcggtccagt agccgcccga cgggtcgggg
     1441 ttgaggttgc agccgatgaa gccgaagtcg cgcacgcagc gctcgagctc ggcgatgcag
     1501 ttcttcggcg acacgcccgg cgtctgcggc agctggcaca cgcccacgaa gttctgcggg
     1561 tagagggcgc acacgcggtg gatcagctcg ttgcagtgct cggaccagtg gcggctcgtg
     1621 gtctcgtcgc cgatgtgatg gcccatgccc gcggcgcgcg gcgagaagat ggtgacgtcg
     1681 gtgccgcgct cgcgctggat cctgagctgg gcgttctcca ggctctcgcg gatctggtcg
     1741 tcgctgatcg cgaggctgcc cttcgaggtg ggcgggtgct tcgcatcctt cagcgcggcg
     1801 atctgcgcgt cgcgccattt ctgcaggtct ttcggagcgg tcgtgtagtg tccgtggcaa
     1861 tcgatgatca tgatgggctc gctgcgggaa aggcgatgct accaagaatc gccgcagggg
     1921 tgaactcgtt gtaacatcgg gtccgatgtg ctcagcccga ctcgtcgcct ttgccgcgtt
     1981 gctcgcgtgc ggcgtcgcct ccgcgcagac gacggatgcc tatcccaccc gtcccatccg
     2041 ctacctggtc gcgagcgcgc cgggcggcat cgccgacatc accgcgcggc tgctcgggcc
     2101 gcatctttcg gaggcgctgc gccagccggt ggtgatcgag aaccgccccg ggagcatcct
     2161 cgtgggcgtc gagctggtgg cgaaggcgcc ggccgacggc tacacgctgc tctcggcgac
     2221 gccgcaggtc gcgatcgcgc agtcgatgta caagcgcgcg agggtcaacc cgcgcgcgga
     2281 gctcgcgccc gtggcgctgg tcggcatgat cccgaacgtg ctcgtcgtcg gcccgcgcac
     2341 gaaagcgaag tcggtcgccg agctggtcga gctcgcgcgt gccaatcccg gcaagctgaa
     2401 ttactcatcg acgggagcgg gcacgtcggt gcacctcgcc gccgagctgc tcaagtacat
     2461 ggccaacgtc gacatcgtgc acgtgcctta ccatggcgcg gccgcggcca tgaccgctct
     2521 catcgccggc gacgtcgaca tgctcgtcga ctcgctgccg ccgtcgctgc cgcacatccg
     2581 cgcggggcgc gtgcgcgcgc tcgcggtgac cagcgcgacg cgcgcgccgc agctgcccga
     2641 cgtgccgacc atggccgagg cgggcttccc cgggttcgag gtgtccgcgt ggtcgggggt
     2701 cgtcgccgcc gcggccacgc cgcccgagat cgtcgcgcgc ctcgagtccg tgatcgggcg
     2761 cgcgctcgag gaccctcgcg ccgccgccac tttcgagaag gcggggctgc cggtgcgctt
     2821 cctcggcgcg aaggatttcg gcgcgttctg ggaccgcgag atcgataagt tcgccctcgc
     2881 cgtgaagcac tccggcgcgg cggtggaata ggagagcgcc gtgagcgcga tcgagcgacg
     2941 cgagatcgag tacgccgtcc acggcggcgc ggcgctgaag ggacacctgt atgcgccgcg
     3001 cggcggcggc cgggtccccg cggtgatcgc ggtgcacggc ggcggctggc ggctggcgaa
     3061 cctcgacaat tatgcgcacc tcggtccctg gctcgccgag cgcggctacg cggtgttcgc
     3121 ggtgacgcac cggctctcga gcgcgtcgac ccgcgtgtac cccga
//
LOCUS       DATDMW010000408         9310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_122932, whole genome shotgun sequence.
ACCESSION   DATDMW010000408 DATDMW010000000
VERSION     DATDMW010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122932"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..729)
                     /locus_tag="VI319_16910"
     CDS             complement(46..729)
                     /locus_tag="VI319_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY6865575.1"
                     /translation="MKLSMIVPALDEEALLPATLAALAPLRGRRHEVIVVDGGSMDRT
                     REIARAGADRVLDAARGRARQMNAGARAAAGDVLVFVHADTLLPPDADECIRRALETG
                     RSWGRFDVRIEGRSAVLGVVAALMNLRSRATGIATGDQAMFVRREAFRRVGGFPDVEL
                     MEDLVLSRALGGVSPPVCLRERVTTSGRRWERGGVLRTVLLMGWLRLRFFLGADPARL
                     ARRYEKKSD"
     gene            complement(730..2871)
                     /locus_tag="VI319_16915"
     CDS             complement(730..2871)
                     /locus_tag="VI319_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6865576.1"
                     /translation="MRRVLVALVLLGLVAAFFALGLQRYFTPEYFQAQRAAIGELRAA
                     RPLASALGFFAVYVAFTSLSIPLAALMSLVCGAIFGLPVGTVIVSFGSSVGATLAFLA
                     SRFLFRDAVRSRLGDRLRTVDRGIEREGAFYLFALRLVPAIPYFAINLLMGLTPIRPW
                     TFYWVSQLAMLPATLVYVNAGTQLAEIRSVSGLLSPALIASFVLLGVFPLIAKKGLDF
                     WKSRRVYSRWTRPAKYDRNLVVIGAGSAGLVSAYIAAAVRAKVTLVERERMGGDCLNT
                     GCVPSKAFIHAARLAAQARRARELGFRSASVDFDFGEIMERVQRVVRAVEPHDSVERY
                     TALGVECLRGEARIASPWTVEVTGADGTRTLTTRAIVIAAGARPLVPPIPGLAEAGCL
                     TSDTVWALRALPRRLAVLGGGPIGCELAQCFARFGSQVTQIEMAPRILGREDPEASAE
                     VARRFEREGIRVLTGHKAAHVLREGGGKVLVCEHGGREVRVECDEILCALGRVANTAG
                     YGLEELGIGTTAQKTIETDDYLQTLYPNIYACGDVAGPWQFTHTASHQAWYAAVNALF
                     GSVKRFRVDDRVIPWTTFTDPEVARVGLSETEARERSVAHEVTRYRLDELDRAIADGE
                     TEGFVKVLTAPGKDRILGATVVGAHAGELIAELVLAMKHGIGLNKILGAIHVYPTFAE
                     ANKYAAGAWKRAHAPKRLLEALGRFHAWMRG"
     gene            complement(2883..3839)
                     /locus_tag="VI319_16920"
     CDS             complement(2883..3839)
                     /locus_tag="VI319_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865577.1"
                     /translation="MAEAKHAPGRSCPPHYRYSPSVFAAPPALRAEALYVVGGLYGNA
                     CALDAVLAMARAEHAALVFNGDFHWFDVDPPGFADVDARVLGHAALRGNVETEIADED
                     SGAGCGCAYPEWVGDGEVERSNAILARLRETARAEPARRARLAGLPMHLVAEVGGVRV
                     GIVHGDAESLAGWLFSQELLARRPERAARALEDARVEVFASSHTCLPVMQSLATARGS
                     VLVANNGAAGMPNFSGERFGVVTRIAPAPSRDALYGTSVGPVRVEAVPVRYDHEAWLE
                     RFDGVWPAGSPAAVSYRERLVSGPRWSLEEAVRIAAPRSRAA"
     gene            complement(3832..4797)
                     /gene="arsS"
                     /locus_tag="VI319_16925"
     CDS             complement(3832..4797)
                     /gene="arsS"
                     /locus_tag="VI319_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732795.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEY6865578.1"
                     /translation="MHATLPLLEISDFPAIRRGRLETLQVNLGYKCNQSCVHCHVNAG
                     PTRTEQMDAATVEIVLRYLAASGAATLDVTGGAPELNPHFRELVRRARALGARVIDRC
                     NLTVLEQPGQEDLAAFLAAERVDIVASLPCYTEELVDRQRGRGVYEASIRALRTLNAL
                     GYGIEGSGLALDLVYNPQGPSLPPAQDALERDYKRILGEKLGVVFNRLYTIANMPIAR
                     FGSALISKGQFNEYMALLRGSFRDENLEAVMCRSLVSVDWRGYVYDCDFNQMLGVPLR
                     VAGRPRVKLESMIGRDLEGNPVAVRDHCYGCTAGQGSSCGGALHG"
     gene            complement(4884..5261)
                     /locus_tag="VI319_16930"
     CDS             complement(4884..5261)
                     /locus_tag="VI319_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865579.1"
                     /translation="MSKKGGGGGKSVTVALHAENKSGETGRAKLTPMGDKTRVELSLK
                     GTPKGVAQPAHIHEGSCAKLDPKPKYPLSNVVDGKSTSEVPVKMEELMEGNMAVNVHK
                     SGDEIKTYVACGDLKTRKGKSKM"
     gene            complement(5480..6268)
                     /locus_tag="VI319_16935"
     CDS             complement(5480..6268)
                     /locus_tag="VI319_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865580.1"
                     /translation="METQATFGAFNQGTTPTIAVFNKAKTPLGVDLDALVAAMQVFID
                     QHVVPVWGTPAKLQRSHGFVKNAWAMVFLDNADQAGALAYHDLTPDGLPISKVFVKTT
                     LDNHDKVSVSASHELVEMLVDPAINLMTTGPDPKVIYAYESADPVEELSFAINGIDMT
                     DFVYPSYFESFHKPGSVQFDHLKKVTKPFQILKGGYQIIFKSGKWSQTYGSAAKAKAF
                     AREDRRQHRSELRKRKKLVQTSEAQVMRAERKAAGARMDDRKAA"
     gene            6417..6968
                     /locus_tag="VI319_16940"
     CDS             6417..6968
                     /locus_tag="VI319_16940"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY6865581.1"
                     /translation="MGHRLSKIVTRTGDAGETGLGDGSRVPKDAPRVAALGEVDELNS
                     CIGLLLAEDPPEAVRAALVEVQHDLLDLGADLSVPGRSTMSEAHVERLDQQLEQLNGG
                     LQPLKEFILPGGTRAAALAHFARTVCRRAERSVVALARKEPVADAGRVYLNRLSDLLF
                     VLGRVLNRAGGRDDVQWQPVRNK"
     gene            complement(6983..7729)
                     /locus_tag="VI319_16945"
     CDS             complement(6983..7729)
                     /locus_tag="VI319_16945"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865582.1"
                     /translation="MPDSLGFRRKFGVVAPSTNTSVQPEFEAFRPHGVTNHFSRVLIP
                     DRKVTDDASFNLMMGDIRASLPAAVEAVMTCSPDYVILGMSSETFWDGMDGSRRLKKR
                     MEKIAGVGVAMGSDACRAALRAYGKVKRLGVITPYMPVGDRQVRRYFTEIGCEVVNLL
                     GLKCSSPTRIAHVSKAALRDAAIAVNRGKVDAIVQVGTNLAMAEVAATAEFWLGKPVI
                     AINTATYWYALRQNGIKDKVYGFGSLLSEH"
     gene            complement(7747..9252)
                     /locus_tag="VI319_16950"
     CDS             complement(7747..9252)
                     /locus_tag="VI319_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY6865583.1"
                     /translation="MESLITNLGIGFGVALSFANLLYCLLGVLLGTLIGVLPGIGPVA
                     TIAMLLPVTFTLPPVSALIMLAGIYYGAQYGGSTTAILVNLPGESSSVVTCLDGYQMA
                     RQGRAGAALGIAALGSFFAGCVATVIIALFAPPLAEVALKFGPAEYFSLMVLGLIAAV
                     VLAHGSVVKAVAMVIFGLLLGLVGTDVNSGVARFNFGISELSDGIGFVSVAMGVFGFA
                     EIIGNLEQKEQREIFTKKVTGLLPTWQDIKDATPSILRGTTLGSALGILPGGGALLAS
                     FAAYTLEKKASKNSAMFGKGAIQGVAAPESANNAGAQTSFIPLLTLGIPSNPVMALMI
                     GAMMIQGIAPGPQVMTERPQLFWGMIASMWVGNLMLVVLNLPLIGMWIKLLTVPYRVL
                     YPSILLFCCIGVYSISNAPFDVMQTAIFGVVGYVFVKLECEPAPLILGFILGPLMEEN
                     LRRAMLLSRGDPVVFFTRPISLTMLVIAGFLLTIVVLPAVRKKREEAFVEA"
BASE COUNT         1441 a         3350 c         3201 g         1318 t
ORIGIN      
        1 gcgcgtcccg gcaccggcgc gcgcgcgaac accacgatgc ggcagctagt ccgacttctt
       61 ctcataccgg cgcgcgagcc gcgccgggtc ggcgccgaga aagaaccgca gccgcagcca
      121 ccccatgagc agcaccgtgc gcagcacgcc gccccgctcc cagcgccgcc ccgacgtcgt
      181 cacgcgctcg cgcaggcaca ccggcggcga cacgcccccg agagcgcgcg agagcaccag
      241 gtcctccatc agctcgacgt cgggaaaccc gcccacgcgg cggaacgcct cgcgccgcac
      301 gaacatcgcc tggtcgccgg tcgcgatgcc ggtcgcgcgc gagcgcaggt tcatcagggc
      361 ggcgacgacg ccgaggaccg cgctgcggcc ctcgatgcgc acgtcgaagc ggccccagga
      421 gcgcccggtc tcgagggcgc ggcggatgca ttcgtccgcg tccggaggga gcagcgtgtc
      481 cgcgtggaca aacacgagca cgtcgcccgc cgctgcgcgc gcgccggcgt tcatctgtcg
      541 cgcgcgcccg cgcgcggcgt cgagcacgcg atccgcgccc gcccgcgcga tctcgcgcgt
      601 gcgatccatc gagccgccgt cgaccacgat cacctcgtgc ctgcgtccgc gcaaaggcgc
      661 caacgctgcg agcgtcgcgg gcagcagcgc ctcctcgtcg agcgccggca cgatcatgga
      721 gagcttcatc tatccgcgca tccacgcatg gaagcgcccg agcgcctcga gcagccgctt
      781 cggcgcgtgc gcgcgcttcc acgcgccggc ggcgtacttg ttcgcctcgg cgaacgtcgg
      841 gtagacgtgg atcgcgccga ggatcttgtt cagcccgatg ccgtgcttca tcgcgaggac
      901 gagctcggcg atcagctcgc ccgcgtgcgc gccgacgacg gtcgcgccga ggatgcggtc
      961 cttgccgggc gcggtgagga ccttgacgaa gccctcggtc tcgccgtcgg cgatcgcgcg
     1021 gtcgagctcg tcgaggcggt accgcgtgac ctcgtgcgcg acgctgcgct ccctggcctc
     1081 ggtctcgctc agccccacgc gcgcgacctc ggggtcggtg aacgtcgtcc acgggatgac
     1141 gcggtcgtcg acgcgaaatc tcttcaccga gccgaacagc gcgttcaccg ccgcgtacca
     1201 cgcctggtgc gaggccgtgt gcgtgaactg ccacgggccc gcgacgtcgc cgcaggcgta
     1261 gatgttcggg tagagggtct ggaggtagtc gtcggtctcg atcgtcttct gcgccgtcgt
     1321 gccgatgccg agctcctcga gcccgtagcc ggcggtgttc gcgacccggc cgagcgcgca
     1381 caggatctcg tcgcactcga cccgtacctc gcggccgccg tgctcgcaca ccagcacctt
     1441 gccgccgccc tcgcgcagca cgtgcgccgc cttgtggccc gtgagcacgc ggatgccctc
     1501 gcgctcgaag cgccgcgcga cctcggcgga ggcctccggg tcctcgcgcc cgaggatgcg
     1561 cggcgccatc tcgatctgcg tcacctgcga gccgaagcgc gcgaagcact gcgccagctc
     1621 gcagccgatc ggcccgccgc ccagcaccgc gagccgccgc ggcagcgcac gcagcgccca
     1681 cacggtgtcc gaggtgaggc agcccgcttc cgcgagcccg gggatcggcg gcaccagggg
     1741 ccgcgcgccc gcggcgatca cgatcgcgcg cgtcgtgagc gtgcgcgtcc cgtccgcgcc
     1801 tgtcacctcg accgtccacg gcgacgcgat ccgcgcctcg ccgcgcaggc actcgacgcc
     1861 gagcgcggtg tagcgctcga ccgagtcgtg cggctcgacg gcccgcacga cgcgctgcac
     1921 gcgctccatg atctcgccga agtcgaagtc gacgctcgcc gagcggaagc cgagctccct
     1981 cgcccgccgc gcctgcgcgg cgagacgagc cgcgtgtata aacgccttcg acggcacgca
     2041 gcccgtgttc aggcagtcgc cgcccatgcg ctcgcgctcg acgagcgtca ccttcgcgcg
     2101 caccgccgcc gcgatgtagg ccgacaccag gcccgcggag cccgcgccga tcacaacgag
     2161 attgcggtcg tatttcgccg gccgcgtcca gcgcgagtag acgcggcgcg acttccagaa
     2221 gtcgagcccc ttcttcgcga tcagcgggaa cacgccgagc agcacgaacg atgcgatcag
     2281 cgccggcgac agcagccccg acaccgagcg gatctcggcg agctgcgtgc cggcgttcac
     2341 gtacacgagc gtagcgggaa gcatcgcgag ctggctcacc cagtagaacg tccacggccg
     2401 gatcggcgtc agccccatca gcaggttgat cgcgaaatac gggatcgccg gcaccagccg
     2461 cagcgcgaac aggtagaacg ccccctcgcg ctcgatcccg cggtcgacgg tgcgcagccg
     2521 gtcgccgagc cgggaacgga ccgcgtcgcg gaacaggaag cggctcgcga ggaaggcgag
     2581 cgtggcgccg acgctggagc cgaacgacac gatcaccgtc cccacgggca gcccgaagat
     2641 cgcgccgcac acgaggctca tcagcgccgc gagcgggatc gagaggctgg tgaacgcgac
     2701 gtacacggcg aagaagccga gggcggaggc gagcggccgc gccgcgcgca gctccccgat
     2761 cgccgcgcgc tgcgcctgaa aatattccgg cgtgaaatac cgctgcagcc cgagcgcgaa
     2821 gaacgccgcg accaggccca ggaggaccag cgcgacgagg acgcggcgca tcgccggtgc
     2881 gatcaggccg cgcgcgagcg cggcgcggcg atgcgcaccg cctcctcgag gctccagcgc
     2941 ggccccgaca cgaggcgctc gcggtacgac accgccgccg gcgatccggc gggccagacg
     3001 ccgtcgaagc gctcgagcca ggcctcgtgg tcgtagcgca ccggcaccgc ctcgacccgc
     3061 acggggccga cgctcgtccc gtagagcgcg tcgcgcgaag gcgccggcgc gatgcgggtg
     3121 acgacgccga accgctcgcc ggagaaattc ggcatgccgg cggcgccgtt gttcgcgacc
     3181 agcaccgacc cgcgcgccgt cgcgagcgac tgcatcaccg gcaggcaggt atgcgaggac
     3241 gcgaacacct cgacccgggc gtcctcgagc gcgcgcgccg cgcgctccgg gcgccgcgcg
     3301 agcagctcct gcgagaacag ccatccggcg agcgattccg cgtctccgtg cacgatgccg
     3361 acgcgtaccc cgccgacctc cgcgacgagg tgcatgggga gccccgcgag gcgcgcgcgc
     3421 cgcgccggct cggcgcgcgc ggtctcgcgc aggcgagcga ggatcgcgtt cgagcgctcg
     3481 acctctccgt ctcccaccca ctccgggtag gcgcagccgc agcccgcgcc cgaatcctcg
     3541 tcggcgatct cggtctcgac gttgccgcgc agcgccgcgt gcccgaggac gcgcgcgtcg
     3601 acgtccgcga agcccggcgg gtcgacgtcg aaccagtgga aatcgccgtt gaagacgagc
     3661 gccgcgtgct cggcccgcgc cattgcgagc accgcgtcga gcgcgcaggc gttgccgtac
     3721 aacccgccca cgacgtagag cgcttcggcg cgcagcgccg gcggcgccgc gaacaccgac
     3781 ggcgaatagc ggtagtgcgg cgggcagctg cgcccgggcg cgtgcttcgc ctcagccatg
     3841 cagcgctccg ccgcagctcg agccctgccc ggcggtgcag ccgtagcagt gatcgcggac
     3901 ggcgaccgga ttgccctcga gatcgcggcc gatcatgctc tccagcttca cccgcggccg
     3961 gccggccacc cgcagcggca cgcccagcat ctggttgaag tcgcagtcgt agacgtagcc
     4021 ccgccagtcg acggagacga ggctgcggca catcaccgcc tcgaggttct cgtcgcggaa
     4081 cgagccgcgc aggagcgcca tgtactcgtt gaactgtcct ttcgagatca gcgccgagcc
     4141 gaagcgggcg atcggcatat tggcgatcgt gtacagccgg ttgaagacga cgccgagctt
     4201 ctcgccgagg atgcgcttgt agtcgcgctc gagcgcgtcc tgcgcgggcg gcagcgacgg
     4261 tccctgcggg ttgtacacga gatcgagcgc gaggcccgag ccctcgattc cgtagccgag
     4321 cgcgttcagg gtccggaggg cgcggatgct cgcctcgtag acgccgcggc cgcgctggcg
     4381 atcgaccagc tcctcggtgt agcagggcag cgacgcgacg atgtcgacgc gctccgccgc
     4441 gaggaaggcg gcgaggtcct cctgcccggg ctgctcgagc accgtcaggt tgcagcggtc
     4501 gatgacgcgc gcgccgagcg cgcgcgcgcg gcgcaccagc tcgcggaaat gcgggttgag
     4561 ctcgggcgcg ccgccggtga catcgagcgt cgccgcgccc gacgcggcga gatagcgcag
     4621 cacgatctcg accgtggccg cgtccatctg ctcggtgcgc gtcggcccgg cgttgacgtg
     4681 gcaatggacg cacgactggt tgcacttgta gccgaggttc acctggaggg tctccaggcg
     4741 cccgcggcgg atcgcaggga agtccgagat ttcgagcagc ggaagcgtcg cgtgcatccg
     4801 cagaggttat caccgaccgc gctcgcgagc cttgacgcgg gcgggccggc cggcggctgc
     4861 gcgccgccgg agaccgcggc aatctacatc ttgctcttgc ccttgcgcgt cttcaggtcg
     4921 ccgcacgcga cgtaggtctt gatctcgtcg ccgctcttgt ggacgttgac cgccatgttg
     4981 ccctccatga gctcctccat cttcaccggc acctcgctcg tcgatttgcc gtccacgacg
     5041 ttcgacagcg ggtacttggg cttgggatcg agcttcgcgc acgagccctc gtggatgtgc
     5101 gccggctgcg cgacgccctt cggcgtgccc ttcaggctga gctcgaccct ggtcttgtcg
     5161 cccatgggcg tgagcttggc cctgccggtc tccccggact tgttctccgc gtgcagcgcc
     5221 accgtgacgc tcttgccgcc gccgccccct ttcttgctca tggaatcctg cgcgaaagcc
     5281 gcgccgctcg ccgtcgccgc ggccgtcaaa agcacccctg cccatagacc gatcgtcata
     5341 ccgcctccta ggaaatgatc cgaaaagagc acgcccgcca agcgcgggcg cgcgcgaaag
     5401 ccggtcccgc ccgaccggac ccaagggatg agcccggatg cgcgtaaaag ttctgtcgat
     5461 cgcgcacgcc ggccgtggtc taggcggcct tccggtcgtc catgcgcgct cccgcggcct
     5521 tgcgctcggc gcgcatgacc tgcgcttccg aagtctgcac gagcttcttg cgcttgcgca
     5581 gctcgctccg gtgctggcga cggtcctccc gggcgaacgc cttcgccttc gcggccgagc
     5641 cgtaggtctg gctccacttg ccgctcttga agatgatctg gtagccgccc ttcaggatct
     5701 ggaagggctt ggtgaccttc ttcaggtgat cgaactgcac cgagcccggc ttgtggaagc
     5761 tctcgaaata cgacgggtag acgaagtccg tcatgtcgat gccgttgatg gcgaagctca
     5821 gctcctcgac cgggtcggcg ctctcgtagg cgtagatgac cttgggatcg gggcccgtcg
     5881 tcatgaggtt gatggcgggg tcgaccagca tctccacgag ctcgtgcgag gccgagacgc
     5941 ttaccttgtc gtggttgtcg agcgtggtct tcacgaacac cttggagatc ggcaggccgt
     6001 cgggcgtgag atcgtgatag gcgagcgcgc ccgcctggtc ggcgttgtcg aggaacacca
     6061 tcgcccaggc gttcttgacg aagccgtgcg agcgctgcag cttcgccggc gtgccccaga
     6121 cggggacgac gtgctgatcg atgaacacct gcatcgcggc gacgagcgcg tcgaggtcga
     6181 cgccgagcgg cgtcttcgcc ttgttgaaca ccgcgatcgt gggcgtcgtg ccctggttga
     6241 atgcgccgaa cgtggcttgc gtttccatgg ccgtctcctt cttgttgtcg gggggaaggc
     6301 gcaatctacg ccagcgaccg ggcgccggca atgccgatgt cattgtcagg aaaaacccaa
     6361 tgcgcgcgtc atccgcgagc cgcgcagcgg tcccgggcgt gtgctagcct gatctcatgg
     6421 ggcaccgcct gtcgaagatc gtcacccgca ccggcgacgc cggcgagacc ggcctcggcg
     6481 acggctcgcg cgtgcccaag gacgccccgc gcgtcgcggc gctgggcgag gtcgacgagc
     6541 tcaactcgtg catcggcctg ctgctcgccg aggacccgcc cgaggcggtg cgcgcggcgc
     6601 tggtcgaggt gcagcacgac ctgctcgatc tcggcgccga cctttccgtg cccggccgct
     6661 cgaccatgag cgaggcgcac gtcgagcgcc tcgaccagca gctcgagcag ctcaacggcg
     6721 gcctgcagcc gctgaaggaa ttcatcctac ccggcggcac gcgcgccgcg gccctcgcgc
     6781 atttcgcgcg cacggtgtgc cggcgcgccg agcgctccgt cgtcgcgctc gcgcgcaagg
     6841 agcccgtcgc cgacgcgggg cgcgtctatc tcaaccggct ctccgacctg ctgttcgtcc
     6901 tggggcgcgt gctcaaccgc gcgggcggcc gcgacgacgt gcagtggcag cccgtgcgca
     6961 acaaatgagg agcggtgctc gctcagtgct ccgataacag cgagccgaag ccgtacacct
     7021 tgtccttgat gccgttctgc cgcaacgcgt accagtaggt cgccgtgttg atggcgatga
     7081 cgggcttgcc gagccagaac tcggccgtcg ccgcgacctc ggccatcgcg aggttcgtcc
     7141 cgacctgcac gatcgcgtcc accttgccgc ggttcaccgc gatcgccgcg tcgcgcaagg
     7201 ccgccttcga gacgtgggcg atccgcgtcg ggctgctgca tttcaggccg agcaggttca
     7261 cgacctcgca gccgatctcg gtgaagtagc ggcgcacctg gcggtcgccg accggcatat
     7321 agggcgtgat cacgcccagg cgcttcacct tgccgtaggc gcgcagcgcc gcgcggcagg
     7381 cgtcggagcc catcgccacg ccgacgccgg cgatcttctc catgcgcttc ttcaggcgcc
     7441 ggctgccgtc catgccgtcc cagaaggtct ccgacgacat gccgaggatg acgtagtcgg
     7501 ggctgcaggt catcaccgcc tcgaccgccg cgggcagcga ggcgcggatg tcgcccatca
     7561 tgaggttgaa gctcgcgtcg tcggtgacct tgcggtccgg gatgagcacg cgcgagaagt
     7621 ggttcgtcac cccgtgcggc cggaaggcct cgaattccgg ctgcaccgac gtgttggtcg
     7681 acggcgcgac gacgccgaac ttgcggcgga agccgagcga gtcgggcacc ctcggctaac
     7741 ccgaaatcac gcctcgacga acgcttcctc gcgcttcttg cgcaccgcgg gcaggaccac
     7801 gatcgtcagc aggaacccgg cgatgacgag catcgtcagg ctgatcggcc gcgtgaagaa
     7861 cacgaccggg tcgccgcgcg agagcagcat cgcgcgcctc aggttctcct ccatgagcgg
     7921 cccgaggatg aagcccagga tcagcggcgc cggctcgcac tcgagcttca cgaagacgta
     7981 gccgaccacg ccgaagatcg ccgtctgcat cacgtcgaag ggcgcgttcg agatcgagta
     8041 cacgccgatg cagcagaaca gcaggatcga ggggtacagc acgcggtagg gcaccgtgag
     8101 cagcttgatc cacatcccga tcagcggcag gttcagcacc acgagcatga ggttgccgac
     8161 ccacatcgag gcgatcattc cccagaagag ctggggccgc tcggtcatca cctgcggtcc
     8221 cggcgcgatg ccctggatca tcatcgcgcc gatcatgagc gccatgacgg ggttcgaggg
     8281 gatgccgaga gtcaggagag ggatgaacga ggtctgcgcg ccggcgttgt tcgcggattc
     8341 cggcgccgcg acgccctgga tcgcgccctt gccgaacatc gccgagttct tcgaggcctt
     8401 cttctcgagc gtgtaggcgg cgaatgaagc gagcagcgcg ccgccgcccg gcaggatgcc
     8461 gagcgcggag ccgagcgtcg tcccgcgcag gatggagggc gtcgcgtcct tgatgtcctg
     8521 ccaggtgggc agcaggcccg tgaccttctt ggtgaagatc tcgcgctgct ccttctgctc
     8581 gaggttgccg atgatctcgg cgaagccgaa cacgcccatc gcgaccgaga cgaagccgat
     8641 gccgtcggag agctccgaga tgccgaagtt gaagcgcgcg acgcccgagt tcacgtcggt
     8701 gccgaccagg cccagcagca gcccgaagat caccatcgcg accgccttga cgaccgagcc
     8761 gtgcgcgagc acgaccgcgg cgatcaggcc gaggaccatc agcgagaagt attcggcagg
     8821 cccgaacttg agcgcgacct ccgcgagcgg cggcgcgaac agcgcgatga tgaccgtggc
     8881 gacgcagccg gcgaagaacg agccgagcgc ggcgatgccg agggccgcgc ccgcgcggcc
     8941 ctggcgcgcc atctggtagc cgtccaggca ggttacgacc gaggaggact cgcccggcag
     9001 gttgaccagg atcgccgtgg tcgagccgcc gtactgcgcg ccgtagtaga tgccggcgag
     9061 catgatcagc gccgataccg gcggcagcgt gaacgtcacc ggcagcagca tcgcgatcgt
     9121 cgccaccggc ccgatgcccg gcagcacgcc gatcagcgtc ccgagcagca cgccgagcag
     9181 gcagtagagc aggttcgcga agctgagcgc gaccccgaac ccgattccga gattcgtgat
     9241 tagggattcc atcgggctac tccaggaagt cgggccagat gttcatgggc aggccgaggc
     9301 ccttcacgaa
//
LOCUS       DATDMW010000409        15879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1117846, whole genome shotgun sequence.
ACCESSION   DATDMW010000409 DATDMW010000000
VERSION     DATDMW010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117846"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VI319_16955"
     CDS             complement(<1..637)
                     /locus_tag="VI319_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY6865584.1"
                     /translation="MKSYWISPNAKGGVDMEQRDVAVPEPGAGQVLVKMRAAGLNRGE
                     LIVGHSVKAGGAAKAAGGEGAGEVAKVGAGVTSVKPGDRVMGRCSGAFAEYALMDARE
                     AIAMPKNLSWEEAASIPLVFCVVHDMLINQGKLQPGEWLLVTGISAGVGVAALQTAKA
                     IGAKVIGTSGSQEKLDRLKPLGLDVGICTRKPDFADAVKKATDGKGANLVVN"
     gene            complement(634..1569)
                     /locus_tag="VI319_16960"
     CDS             complement(634..1569)
                     /locus_tag="VI319_16960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6865585.1"
                     /translation="MGGRFAQGFAAVLATAVLWGVQFPVAKDAFAAVDAYHVTAIRYG
                     LGTLLLIPMFVAQEGRTALDYRGRFWAATVFGLIGMCASPMLVFVGIAMSRPAHAAVI
                     VSLQPSMAALADWALRGRRPANFTLGCIAVAFLGVLAVVTKGHPATMFGKGELAGDFV
                     ILLGAACWVVYTMATETFRGWSALRFTVLTIVPGTIGCIVVTAALDAAGIATLPGTAA
                     LASVWWQLAYLTIGGIVVSMICWNWGNQRIGALNTMLLLNAVPVVTFATGLAQGHRFE
                     PVELAGALVVIAALVANNFYLRRASARKATKESLA"
     gene            complement(1586..2989)
                     /locus_tag="VI319_16965"
     CDS             complement(1586..2989)
                     /locus_tag="VI319_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865586.1"
                     /translation="MRSMVVLSLVFACGAAFAQAGKEEKRNMELVGYSDLQGRTAYQP
                     TIRKQGNRWIAYVGHHGDNLLNPLTGKKEDNGTSIVDVTDPAKPRILAHIPGEPGKAE
                     AGGAQMVRVCAGSELPKGDKSKTYLLRTFGNQAHEIWDVSDPAKPSLLTTVVKGLKGT
                     HKSWWECDTGIAYLVSGIEGWRVRRMTQVWDLSDPAHPVHIRDFGLPGQQAGKPGEPP
                     ADLHGMISTGPKGNRVYFGYGTNKDGVLQIVDREKLINGPKEPTDENLVYPQVGRLDM
                     PPTNGAHTTLPLLGMTIGEFAKDKGGAKRDFVMIVDEEIANECLEPRQMVWMVDITTE
                     SKPFGVGNFTVPEKSGNFCARGGRFGSHSPNENQPPMYAGRLVFVAWFNAGVRAIDVR
                     DPYHPREVGYYIPAMTKATDKRCVGKGAEERCKRAIQTNNVDVDDRGYIYIVDRANTG
                     MHILRVTGEARKIAKFQ"
     gene            complement(3013..3510)
                     /locus_tag="VI319_16970"
     CDS             complement(3013..3510)
                     /locus_tag="VI319_16970"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6865587.1"
                     /translation="MEKRIYDAGIESSSAPFNLCVRYGDLLFISGLPPFDAGFTRAVR
                     EARENGRPIPPFPDMPFEAQVRLVLDNMKKLLEQAGSSMDCLLKVIVWLKDQRQAEEF
                     DRVYRGYFSSREALPARTRIQAGRTPMDCGLEVEAIGYVPKKAKSRKTKPRASRKTSR
                     RPRRR"
     gene            3582..3968
                     /locus_tag="VI319_16975"
     CDS             3582..3968
                     /locus_tag="VI319_16975"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEY6865588.1"
                     /translation="MTIALALLAGACGALAATPMRAGLWEITARIQGGLPPITSRTCI
                     SAKDAEAMGRGRAANKGAENCEPVDYRQEGNKATWALSCKGRTPMTGAGSIEYGNDTF
                     TQKVMLKSSEGTMTMESTGRRVGDCR"
     gene            complement(4011..4910)
                     /locus_tag="VI319_16980"
     CDS             complement(4011..4910)
                     /locus_tag="VI319_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY6865589.1"
                     /translation="MTRIGVDIGGTKTELVALADDGRVLAQRRAPTPRAEYPEAMRQL
                     AELVLEIERDTGGRCTVGIGTPGRVSRETGILSNAFALVFNERPIKRDLEMLLQREVR
                     FANDANCFALSEASDGAARGARIVFGVILGTGVGSGIVVDGRVLEGANSIAGEWGHNP
                     LPWPRDDERPGPRCGCGQPGHIEAFLSGPGLARDHAACTGEKLSPAEIVERARAGEGA
                     CAESLARYEERLGRALAHVINVIDPDVIVVGGGLSGIPGLTLDVPQRWAGYVYAPAVM
                     TRLVPAMHGDASGVRGAAWLWPA"
     gene            complement(4939..5736)
                     /gene="otsB"
                     /locus_tag="VI319_16985"
     CDS             complement(4939..5736)
                     /gene="otsB"
                     /locus_tag="VI319_16985"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY6865590.1"
                     /translation="MPRTQQQLAVRDELYRVPRFSRDWALFLDVDGTLLEIAPHPAAV
                     KVSPPLRQLLGRLCGAAGGALALVSGRSVADVESLFAPLRLCVAGQHGAERRDFAGRL
                     HRQVRQPVGLRVAAERLGRFVAERPGLVIENKGLNIAVHYRNAPGYEREVAEEFAATI
                     AELGEDFELQAGKMVYELKPAGQSKGAAIAQFMREEPFQGRVPVFVGDDLTDESGFAV
                     ANKLGGHSIKVGAGDSVAAWRLPDPEGVRAWMRALALEWARARRAGG"
     gene            5917..6852
                     /locus_tag="VI319_16990"
     CDS             5917..6852
                     /locus_tag="VI319_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6865591.1"
                     /translation="MSERPFPRRAVLRLGALSLASLLMPDTPFQPARAEAALLTRPIP
                     STGEPLPAVGLGTWQSFDVAGDAAAVEEARETLRALVAAGGRVVDSSPMYGSAEAVAG
                     QLVQELGLRERIFVATKVWTEGRNAGRAQMDASMKKLRVERLDLMQVHNLVDAGTHLQ
                     TLREWKAAGRVRYVGVTHYHAGAHAALETLVRSGEVQFVQVNYSLEEPEAERRLLPAA
                     ADSRVAVIVNRPFAEGSMFRRVKGKPLPAFAAELGCATWAQYFLKWILGHPAVTCAIP
                     GTRNPKHLADNLAAARGPVPDAAARKRMAEHFASL"
     gene            6947..8128
                     /locus_tag="VI319_16995"
     CDS             6947..8128
                     /locus_tag="VI319_16995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6865592.1"
                     /translation="MRTLYLLMLLSVLNHTTFAGTRVAVALYAIRLGETPFTVGTLMA
                     LYALLPMLFAVSIGRLTDRFGARAPMLAGSTLLAAGASIPFAWPGIASLYLSSVLIGS
                     SFMLYHVAYQNVVGYVGAPEDRPINFSIAALGFSVSGFSGPMLAGFGIDHIGIPGTFL
                     VLSLLPLVPLAVLALDKVRLPRLPGHGAAPAQNRRLADLFRHRELRRLFVVSGLLAMA
                     WDLFNFAVPIYGSRVELSASKIGAILGAFSVATFVVRTFLPAISRRLTAWPLLVVSLV
                     VAGTSFVLFPLFRSAAPLMAVAFFLGLGLGMSQPMVMSLMHNATPPGRVGEAVGVRMT
                     LVNMSQTGMPLLFGALGSVLGTLAPAFWAMALLLLAGGAFAKRYHDRRAKAAGAPDAT
                     L"
     gene            8144..8776
                     /gene="pdxH"
                     /locus_tag="VI319_17000"
     CDS             8144..8776
                     /gene="pdxH"
                     /locus_tag="VI319_17000"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY6865593.1"
                     /translation="MDLAHLRREYMRAGLEESALAADPLEQFARWLDDAVRAEVPLAN
                     AMTLATATRSGRPSARAVLLKGFDARGFVFFTNYDSRKGRELAENPRAMLLFCWEPLE
                     RQVRIEGAVERTDAAESDAYFASRPLGARLSAVVSPQSEPVAGRGELEASYAAAAKRL
                     GDSPARPAHWGGYRVVPEWFEFWQGRQDRLHDRLCYTRAGSTWRVGRLAP"
     gene            complement(8835..9251)
                     /locus_tag="VI319_17005"
     CDS             complement(8835..9251)
                     /locus_tag="VI319_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865594.1"
                     /translation="MKKCTLLAWMVAAIASAPVALAQGVTDGWMNPYHVAGEKLDSGL
                     GDIKSVGAYANPYHVAGEKSDSGLGNISAAELAPYMGSSHVAGEKLDSGLGELTAADI
                     APYMNPYHVAGEKLDSGLGEIAAQRSSGAIIRASSY"
     gene            9384..10664
                     /locus_tag="VI319_17010"
     CDS             9384..10664
                     /locus_tag="VI319_17010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6865595.1"
                     /translation="MSIISRVARIEPGEGRALAWSFAYFFCLLCGYYILRPLRDEMGI
                     QSGVANLPGLFSVTFAATLALVPVFGWAAARLPRRRLVPWTYLFFVAHIGVFYALFEG
                     GAARPAVAAAFFVWVSVFNLFVVSVFWSLMADLFTPEQAARLFGVVSAGGTSGALAGP
                     ALTAALVGPLGTANLLLLSGAFLAACLVCIHALLRWARGREPAGAARPPEVRIGGTTW
                     SGLAEIARSPYLAAIVAYVLLYTALLGFAYFELARMIAERYADPAERTALFAHVDLAV
                     NVLTLLGQLLVVGRLVERLGVGAALALLPLLALAGFSAMGAAPVLAVMLVFQVARRAA
                     DYAIARPAREMLFTVLDRNAKYKSKNFIDTVVFRGGDAASGWLYAGFKALGLALPGMA
                     LAAIPGVLAWLALGIFLGRRYEGLARRGVATSGR"
     gene            complement(10661..11983)
                     /gene="phoR"
                     /locus_tag="VI319_17015"
     CDS             complement(10661..11983)
                     /gene="phoR"
                     /locus_tag="VI319_17015"
                     /inference="COORDINATES: protein motif:HMM:NF008235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEY6865596.1"
                     /translation="MNDPLVRTLAALALLAVPALAAGLLFGPAWGWAVFSLGLAAVLL
                     SHARHLRLLARWAAGLAPDVPEGTGMWQEALTLLYRRERAGLRRRRALARLLARSRRA
                     GRALPYGMAILDAEHRILWCNDSSERHLGIDVRSDIGRPIVNLVRQPEFVRYVAARDF
                     SAPLALRTARDDGLILSVQLVPYVESQWLLLSRDVTQATRLETMRRDFVANVSHELRT
                     PLTVLIGFLETVRELKLDPERSRDYLNLMAEQGRRMQRIIDDLLTLSTLESAPAPEHD
                     ERVDVALLLARVLGEAQALSAGRHRVRLDAEEGFDLLGAENELASAFGNLASNAVRYT
                     PAGGEVRLVWRASPEGATFAVEDTGLGIEREHLPRLTERFYRVDRGRSRESGGTGLGL
                     AIVKHALARHQGALEIVSEPGRGSRFAARFPARRVVAAPARRKSEALG"
     gene            complement(11997..12371)
                     /locus_tag="VI319_17020"
     CDS             complement(11997..12371)
                     /locus_tag="VI319_17020"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY6865597.1"
                     /translation="MEQESPFKGKTGLRRLINALGYSLEGVRSALRHEAAFRQELLLA
                     AVLIPAALLAPVSAPARALMIACVLLVLVVELLNSALEAVVDLASPEHHRLAKRAKDL
                     GSAAVFLSLVNVAVVWAIALFG"
     gene            complement(12400..14541)
                     /gene="ppk1"
                     /locus_tag="VI319_17025"
     CDS             complement(12400..14541)
                     /gene="ppk1"
                     /locus_tag="VI319_17025"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY6865598.1"
                     /translation="MLQPVRLPSRRVRPQFLPRELGMLEFNRRVLAQAEDRRVPTLER
                     LRFLCIVSNNLDEFFEIRVAEVKEKIKLGKEIEITGETPASEVFAAISERAHALVDDQ
                     YRVLNREVLPALADDGVLFLSEQQWTPDQRAWVREYFVREMMPLLTPIGLDPAHPFPR
                     VLNKSLNFAVQLEGTDAFGRSSGKAIVQAPRLLPRAIRLPADLAGDAYAFVLLTSALQ
                     AFVGMLFQGMSVLGSYPFRVTRNSDLWLDEEEVKNMRIALQGELPQRHFGDSVRLEIA
                     ADMPAGMEQFLLEQFELAPPDLYRVDGPVNLVRLMEIPELVDKPRLKYPPFSPGVPRP
                     LDKGADLFRAIRKADILLHHPFQSFNPVIAFLEQSVEDPDVVAIKQTVYRTGTDSVLM
                     RYLIDAAQRGKEVTVVVELLARFDEEANLNWAAKLEEVGAHVVYGVVGYKTHAKMSLV
                     MRRENGTLRRYAHLSTGNYHPRTAKQYTDFGLFTADESICDDVNEVFKQLTGLGRARR
                     LNHLWISPFTLHERVLAAIRNEAAHAREGKPAHIIAKMNALLEPKVIEALYKASQAGA
                     KIDLVIRGVCALRPGIPGLSDNIRVRSVVGRFLEHSRIFYFLNDGAEDVYLASADWME
                     RNFFRRIELCVPVLDPKLKNRVIREGLRPYLSDNSQAWEMDADGNYRLRRSRGRLPRS
                     AQEQLLAMLATTASGALQATTPAGRRRPRTP"
     gene            14679..>15879
                     /gene="ppx"
                     /locus_tag="VI319_17030"
     CDS             14679..>15879
                     /gene="ppx"
                     /locus_tag="VI319_17030"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY6865599.1"
                     /translation="MKPDTLAALDLGSNSFHLQIARVLDDQVYMLDSLREPVRLGGGL
                     TRDRRIDRRTRARAMAALERFAERLRGFPRGSVRAVGTSALRTAKNASAFIAQAERIL
                     GYPVEVIFGREEARLIYLGVAHELAASDERRLVVDIGGGSTECIIGKGLEPQLMESLP
                     MGCVGYSLRFFAEGRLDKTRFERAERAAANELQRIVDAYRAAGWTHAVASSGTARALA
                     SIVHGCGLAEHGLTASALAELRDRFIRAGRIGRLKIPGLREDRKPIIAGGLAIMTAVL
                     AQFGIDALDVSDAALRQGVMYDLLGRVRHRDMREATVRQFMRRYQVDSAQAERVAALA
                     QRVLADIDPKASESDAALLRWAASLHEIGISIAHAGYHKHSAYIVSQADMPGFSQSEQ
                     ARLARLVL"
BASE COUNT         2179 a         5705 c         5556 g         2439 t
ORIGIN      
        1 tgttgaccac gaggttcgcg cccttgccgt cggtcgcctt cttcaccgcg tcggcgaagt
       61 cgggcttgcg cgtgcagatc ccgacgtcga ggcccagcgg cttgagccgg tcgagcttct
      121 cctgcgagcc cgaggtgccg atcactttcg cgccaatcgc cttcgcggtc tgcagcgcgg
      181 cgacgccgac gcccgcggag attccggtca cgagcagcca ctcgcccggc tgcagcttcc
      241 cctggttgat cagcatgtcg tgcacgacgc agaacacgag cgggatgctc gccgcctcct
      301 cccacgacag gttcttcggc atcgcgatcg cctcgcgcgc gtccatcagc gcgtactcgg
      361 cgaacgcgcc cgagcagcgg cccatgacgc ggtcgccggg cttgacgctg gtgacgcccg
      421 cgccgacctt cgcgacctcg cccgcgccct cgccgcccgc ggccttcgcg gcgccgcccg
      481 cctttaccga gtggccgacg atcagctcgc cgcggttcag gcccgccgcg cgcatcttca
      541 cgagcacctg gcccgcgccc ggctcgggca ccgcgacgtc gcgctgctcc atgtcgacgc
      601 cgcccttggc gttcgggctg atccagtacg atttcatgcg aggctctcct tggtggcttt
      661 gcgcgccgac gcgcggcgca gatagaaatt gttggcgacg agcgcggcga tcacgaccag
      721 tgccccggcg agctcgaccg gctcgaagcg gtgcccctgc gcgaggcccg tggcgaacgt
      781 cacgaccggc accgcgttga gcagcagcat cgtgttcagc gcgccgatgc gctggttgcc
      841 ccagttccag cagatcatcg agacgacgat gccgccgatc gtgaggtagg cgagctgcca
      901 ccagacggag gcgagcgccg ccgtccccgg cagcgtcgcg atgcccgcgg cgtcgagcgc
      961 cgcggtcacg acgatgcagc cgatcgtacc cggcacgatc gtgagcacgg tgaagcgcag
     1021 cgccgaccag ccgcggaagg tctcggtcgc catggtgtac acgacccagc acgccgcgcc
     1081 gagcaggatc acgaagtcgc cggcgagctc gcccttcccg aacatcgtcg ccgggtggcc
     1141 cttggtcacg accgcgagca cgccgaggaa cgcgaccgcg atgcagccga gcgtgaagtt
     1201 cgccgggcgc cgtccgcgca gcgcccagtc cgcgagcgcc gccatcgacg gctgcagcga
     1261 gacgatgacc gccgcgtgcg ccggccgcga catcgcgatg cccacgaaca cgagcatcgg
     1321 ggatgcgcac atgccgatga ggccgaagac cgtcgccgcc cagaagcggc cgcggtagtc
     1381 gagggcggtg cgcccctcct gcgccacgaa catcgggatg aggagcagcg tcccgagccc
     1441 gtagcggatc gcggtgacgt ggtacgcgtc gaccgccgcg aacgcgtcct tcgccaccgg
     1501 gaactgcacg ccccagagga ccgccgtggc gagcacggcg gcgaagccct gggcgaagcg
     1561 tccgcccatc aggacgcggg cgcggtcact ggaacttcgc gatcttgcgc gcctcgcccg
     1621 tcacccgcag gatgtgcatg cccgtgttcg cgcggtcgac gatgtagatg tagccgcggt
     1681 cgtcgacgtc gacgttgttc gtctggatcg cgcgcttgca gcgctcctcg gcgcccttgc
     1741 cgacgcagcg cttgtcggtg gccttcgtca tcgccgggat gtagtagccg acctcgcgcg
     1801 gatggtacgg gtcgcgcacg tcgatggcgc gcacgccggc gttgaaccac gcgacgaaga
     1861 cgaggcgccc ggcgtacatc ggcggctggt tctcgttcgg cgaatgcgag ccgaagcgcc
     1921 cgccgcgcgc gcagaaattt ccggatttct cgggtacggt gaaattcccg acgccgaacg
     1981 gcttcgactc ggtagtgatg tcgaccatcc acaccatctg ccgcggctcg aggcattcgt
     2041 tcgcgatctc ctcgtcgacg atcatcacga agtcgcgctt cgcgccgccc ttgtccttcg
     2101 cgaactcgcc gatcgtcatg ccgaggagcg gcagcgtcgt gtgcgcgccg ttcgtcggcg
     2161 gcatgtcgag gcgcccgacc tgcgggtaga cgaggttctc gtcggtcggc tccttcggcc
     2221 cgttgatcag cttctcgcgg tcgacgatct gcagcacgcc gtccttgttg gttccgtaac
     2281 cgaaatagac gcggttgccc ttcgggccgg tcgagatcat gccgtgcagg tcggcgggcg
     2341 gctcgcccgg cttgcccgcc tgctgcccgg gcagcccgaa gtcccggatg tggaccgggt
     2401 gcgcgggatc cgagagatcc cagacctgcg tcatgcgccg cacgcgccag ccctcgatcc
     2461 ccgacacgag ataggcgatg ccggtatcgc attcccacca gctcttgtgc gtgcccttca
     2521 ggcccttcac caccgtcgtc aggagcgacg gcttcgccgg gtcgctcacg tcccagatct
     2581 cgtgcgcctg gttgccgaaa gtgcgcagca ggtaggtctt cgacttgtcg cctttcggca
     2641 gctcgctgcc cgcgcacacg cgcaccatct gcgcgccgcc ggcctcggcc ttgcccggct
     2701 cccccgggat gtgcgcgagg atgcgcggct tcgccggatc ggtgacgtcg acgatcgagg
     2761 tgccgttgtc ctccttcttg ccggtgagcg ggttgagcag gttgtcgccg tggtgcccga
     2821 cgtaggcgat ccagcgattg ccctgcttgc ggatcgtcgg ctggtacgcg gtgcgtccct
     2881 gcaggtcgct gtacccgacc agctccatgt tgcgcttctc ttccttcccc gcctgcgcga
     2941 aagccgcgcc gcacgcgaaa acgagcgaca gaacgaccat ggacctcatt gcttttctcc
     3001 tcggtggaaa gatcagcgcc gtcgcgggcg ccgggacgtt tttcgcgaag cccgcggctt
     3061 cgtcttcctg gacttcgcct tcttcggcac gtagccgatc gcctcgacct cgaggccgca
     3121 atccatgggc gtgcggcccg cctggatgcg agtgcgcgcg ggcagcgcct cgcggctcga
     3181 gaagtagccg cggtagacgc ggtcgaactc ctcggcctgg cgctggtcct tcagccacac
     3241 gatgaccttc agcaggcaat ccatgctcga gccggcctgc tcgagcagct tcttcatgtt
     3301 gtcgaggacg aggcgcacct gcgcctcgaa cggcatgtcg gggaacggcg ggatcggccg
     3361 gccgttctcc cgagcttcgc gcaccgcgcg cgtgaacccg gcgtcgaacg gcggcagccc
     3421 ggagatgaac agcagatcgc cgtagcgaac gcaaaggttg aacggggcgc tcgaggattc
     3481 gatgcccgcg tcgtagattc gcttttccat gggagccggt cgcgcggaag gaatagtatg
     3541 atgacggcac cgtccgcgca acggaggcca cgatgtcccg catgacgatc gctctcgcgc
     3601 tgctcgccgg cgcctgcggc gctctcgcgg cgacgccgat gcgcgccggc ctctgggaga
     3661 tcacggcccg gatccagggc ggcctgcccc cgatcacctc gcgcacctgc atcagcgcca
     3721 aggacgccga ggcgatgggc cgcggccgcg ccgcgaacaa gggcgccgag aactgcgagc
     3781 cggtcgatta ccgccaggaa ggcaacaagg cgacctgggc gctgtcgtgc aaaggccgca
     3841 cgcccatgac cggcgccggc tcgatcgagt acggcaacga caccttcacg cagaaggtca
     3901 tgctcaagag cagcgagggc acgatgacga tggagagcac cggccgccgc gtcggcgatt
     3961 gtcgctgatg tcagcctgag ggcgaagccc gaagaatctg cttttcgcct ttacgcaggc
     4021 cagagccacg ccgccccgcg caccccgctc gcatcgccgt gcatcgccgg gacgagccgt
     4081 gtcatgactg ccggcgcgta gacgtagccg gcccagcgct gcggcacgtc aagggtgagg
     4141 cccgggatcc ccgagagccc tccgcccacg acgatcacgt ccgggtcgat gacgttgatg
     4201 acgtgggcga gcgcgcgtcc caggcgctcc tcgtagcgcg cgagcgactc ggcgcacgcg
     4261 ccctcgcccg cccgggcgcg ctccacgatc tcggccggcg acagcttctc gccggtgcag
     4321 gctgcgtggt cgcgcgcgag ccccggcccc gacaggaagg cctcgatgtg gccgggctgc
     4381 ccgcagccgc agcgcggtcc cgggcgctcg tcgtcgcggg gccacggcag cgggttatgt
     4441 ccccactcgc cggcgatcga attggcgcct tcgagcacgc gcccgtcgac cacgatgccg
     4501 ctgccgacgc ccgtcccgag gatcacgccg aagacgatgc gcgcgccgcg cgccgcgccg
     4561 tcggaagctt ccgacagcgc gaagcagttc gcatcgttcg cgaagcgcac ctcgcgctgc
     4621 agcagcatct cgaggtcgcg cttgatcggc cgctcgttga acaccagcgc gaacgcgttc
     4681 gacaggatcc cggtctcgcg cgacacgcgt cccggcgtgc cgatgcccac cgtgcagcgc
     4741 ccgcccgtat cgcgctcgat ctcgagcacc agctcggcga gctgcctcat cgcttcggga
     4801 tattcggcgc gaggagtcgg cgcgcgccgc tgcgccagga cgcggccgtc gtcggcgagc
     4861 gcgaccagct cggtcttggt gccgccgatg tcgacgccga tgcgggtcat gcgtcgcgcc
     4921 gaagcgggcg cggccggctc agccgcccgc gcgccgcgca cgcgcccact cgagcgcgag
     4981 cgcgcgcatc cacgcgcgca cgccctcggg atcgggcagc cgccacgcgg cgaccgagtc
     5041 gcccgcgccg accttgatcg aatggccgcc gagcttgttc gccacggcga atcccgactc
     5101 gtcggtgaga tcgtcgccga cgaacaccgg cacgcggccc tggaacggct cctcgcgcat
     5161 gaactgcgcg atcgccgcgc ccttgctctg cccggcgggc ttgagctcgt agaccatctt
     5221 gcccgcctgc agctcgaagt cctcgccgag ctcggcgatg gtcgcggcga actcctcggc
     5281 gacctcgcgc tcgtagccgg gcgcgttgcg gtagtgcacc gcgatgttga gccccttgtt
     5341 ctcgatcacc agcccggggc gctcggcgac gaagcggccc agccgctcgg cggcgacgcg
     5401 caggcccacg ggttggcgca cctggcggtg caggcggccg gcgaagtcgc gccgctcggc
     5461 gccgtgctgc ccggccacgc atagcctgag cggcgcgaac aggctctcga cgtcggcgac
     5521 cgagcgcccg ctgaccagcg cgagcgcgcc gcccgcggcg ccgcacaggc gtcccaggag
     5581 ttgccgcagg ggcgggctca ccttgacggc ggcggggtgc ggcgcgatct cgagcagggt
     5641 gccgtcgacg tccaggaaca gcgcccagtc gcgggagaaa cgcggcaccc tgtagagctc
     5701 gtcgcgcacg gcgagttgtt gttgcgtcct cggcatgggg atcagcgggg aacgtcgccg
     5761 ccgcggtccg gtctcacctc ggtcggacgg gcgggccggc aggaccggac ggaccagcgc
     5821 gttgcaatca tacctgctct ttcgagtgcg attcggcgat tgcggttcac gggccggatc
     5881 gcctacgatg ccgggattgc gaccgcctcg ccatccatgt ccgagcgtcc cttccccagg
     5941 cgcgccgtgc tccggctcgg cgcgctctct cttgcgagcc tgctcatgcc cgacacgccg
     6001 ttccagcccg cccgcgccga ggcggccctg ctgacccgcc ccataccgtc cacgggcgag
     6061 ccgcttccgg ccgtcggcct cggcacgtgg cagtctttcg acgtcgcagg cgacgccgcc
     6121 gccgtcgaag aggcgcgcga gaccctgcgc gcgctggtcg ccgccggcgg ccgcgtcgtc
     6181 gacagctcgc cgatgtacgg gtcggccgag gccgtcgcag gacagctcgt gcaggagctc
     6241 ggcctgcgcg agcgcatctt cgtcgcgacc aaggtctgga ccgagggacg caacgcgggc
     6301 cgcgcccaga tggacgcgtc gatgaagaag ctgcgcgtcg agcggctcga tctcatgcag
     6361 gtgcacaacc tggtcgacgc cgggacgcac ctgcagacgc tgcgcgaatg gaaggccgcc
     6421 gggcgcgtgc gctacgtcgg cgtcacgcac taccacgccg gcgcgcacgc cgcgctcgag
     6481 acgctcgtgc gctcgggcga ggtgcagttc gtccaggtga actattcgct cgaggagccc
     6541 gaggccgagc ggcgtctcct ccccgcggcg gccgacagcc gcgtcgccgt catcgtcaac
     6601 cgccccttcg cggaagggtc gatgttcagg cgcgtgaaag gcaagcccct gcccgccttc
     6661 gccgcggagc tgggctgcgc cacctgggcg cagtatttcc tgaagtggat cctcggccac
     6721 ccggcggtga cctgcgcgat cccgggcacg cgcaacccga agcacctcgc cgacaacctc
     6781 gcggccgcgc gcgggccggt tcccgacgcc gcggcgcgca agcgcatggc cgagcacttc
     6841 gcctcgctct gatgcgaacg cgcccggggc gcgggcgttc gcgtagaatt tgcgtccgag
     6901 aacaacacgc gccgccgacc gggcggcgcg ccgcctcgag ccttccgtgc gcacgctcta
     6961 tctcctgatg ctgctgtcgg tgctgaacca cacgacgttc gccggaacgc gcgtggcggt
     7021 ggcgctgtat gcgatccgtc tcggcgagac gccgttcacc gtcggcacgc tgatggcgct
     7081 ctacgcgctg ctgcccatgc tgttcgccgt gagcatcggg cggctgaccg accgcttcgg
     7141 cgcgcgcgca ccgatgctcg cgggctcgac gctgctcgcg gccggcgcct cgatcccgtt
     7201 cgcgtggccg ggcatcgcgt cgctctatct ctcgagcgtg ctgatcggat cgagcttcat
     7261 gctctaccac gtggcgtacc agaacgtggt cggctacgtc ggcgcgcccg aggaccggcc
     7321 gatcaatttc agcatcgcgg cgctgggctt ctcggtctcg ggcttctccg ggccgatgct
     7381 cgccgggttc ggcatcgacc acatcggcat cccgggcacc ttcctcgtgc tctcgctgct
     7441 gccgctcgtg ccgctcgctg tcctcgcgct cgacaaggtg cggctgccgc ggcttccggg
     7501 gcacggcgcg gcgccggccc agaaccggcg actcgccgac ctgttccggc accgcgagct
     7561 gcggcggctg ttcgtcgtga gcggcctgct cgcgatggcg tgggacctgt tcaacttcgc
     7621 ggtgccgatc tacggctcgc gcgtcgagct gtcggcctcg aagatcggcg ccatcctcgg
     7681 cgccttctcg gtggccacct tcgtcgtgcg caccttcctg cccgcgatct cgcggcggct
     7741 caccgcgtgg ccgctgctgg tcgtgtcgct cgtggtcgca gggacgagct tcgtgctgtt
     7801 cccgctgttc cgcagcgcgg caccgctcat ggcggtcgcc ttctttctcg ggctcgggct
     7861 cgggatgtca cagccgatgg tcatgtcgct catgcacaac gcgacgccgc ccgggcgcgt
     7921 cggcgaggcg gtcggcgtgc gcatgacgct cgtcaacatg agccagaccg ggatgccgct
     7981 cctgttcggc gcgctcggct cggtgctcgg cacgctcgcg cccgcgttct gggcgatggc
     8041 gctgctgctg ctcgcgggcg gtgccttcgc caagcgctac cacgaccgac gcgcgaaggc
     8101 cgcaggggcc ccggacgcca cgctgtagaa tccggccgtg ccgatcgatc tcgcccacct
     8161 gcgccgcgag tacatgcgcg ccgggctcga ggagtccgcg ctggctgccg acccgctcga
     8221 gcagttcgcg cgctggctcg acgacgccgt gcgcgccgag gtgccgctcg cgaacgcgat
     8281 gacgctcgcc acggcgaccc gctcgggacg cccgtcggcg cgcgcggtgc tgctgaaggg
     8341 tttcgacgcg cgcggcttcg tcttcttcac caactacgac agccgcaagg ggcgcgagct
     8401 cgccgagaac ccgcgcgcga tgctgctgtt ctgctgggag ccgctcgagc gccaggtccg
     8461 catcgaaggc gcggtcgagc gcaccgacgc cgccgagtcc gacgcctact tcgcgagccg
     8521 gccgctcggc gctcgcctct cggcggtcgt ctcgccgcag agcgagccgg tcgcgggccg
     8581 cggcgagctg gaggcgagct acgccgccgc cgccaaacgc ctcggcgact cgccggcgcg
     8641 gcctgcgcac tggggcggtt atcgcgtggt cccggagtgg ttcgagttct ggcagggccg
     8701 ccaggaccgg ctgcacgacc ggctgtgcta cacgcgcgcg ggaagcacct ggcgcgtcgg
     8761 gcgcctcgcg ccctgacaaa cgaaaggccg gggccgcccg aatgctgcgg ccccgaccgg
     8821 gagcaacttt gacgctagta cgacgaggcg cggatgatgg cgccgctcga ccgctgcgcc
     8881 gcgatctcgc ccaggccgct gtcgagcttc tcgcccgcga cgtggtacgg gttcatgtac
     8941 ggcgcgatgt cggcggccgt cagctcgccg aggccgctgt cgagcttctc gcccgcgacg
     9001 tggctcgaac ccatgtacgg cgcgagctcg gcggcgctga tgttgcccag gccgctgtcc
     9061 gacttctcac ccgccacgtg atacggattg gcgtaggcgc cgaccgactt gatgtcgccc
     9121 aggccgctgt ccagcttttc gcccgccacg tgatacgggt tcatccagcc gtccgtcacg
     9181 ccctgcgcca gcgccaccgg cgcggacgcg atcgctgcca ccatccaagc caacagtgtg
     9241 cactttttca tgttcttccc ctttgatcgt cgtggccgtc cgttgggccc gcgtcatgcg
     9301 cgacgaggat cggccacttc gctcctcgaa acgtgctcca acgacgctcg ctatcccaag
     9361 gagcaagacc ggtgccaggg tccatgagca ttatttctcg cgtcgcgcgc atcgagccgg
     9421 gcgaaggcag ggcgctcgcg tggtcgttcg cctatttctt ctgcctgctg tgcggctact
     9481 acatcctgcg gccgctgcgc gacgagatgg gcatccagag cggcgtcgcc aatctcccgg
     9541 ggctgttctc ggtcacgttc gccgcgacgc tcgcgctggt tcccgtgttc ggctgggcgg
     9601 cggcgcggct gccgcgccgc cgcttggtgc cctggacgta cctcttcttc gtcgcccaca
     9661 tcggcgtctt ctacgcgctg ttcgagggcg gcgcggcgcg cccggcggtc gcggcggcgt
     9721 tcttcgtgtg ggtgagcgtc ttcaatctct tcgtcgtgtc cgtgttctgg agcctgatgg
     9781 ccgacctgtt cacgcccgag caggcggcgc ggctcttcgg cgtggtgtcc gcgggcggca
     9841 cgagcggtgc gctcgcgggc ccggcgctga ccgcggcgct ggtcggcccg ctcggcaccg
     9901 cgaacctgct cctgctctcg ggcgcgttcc tcgcggcgtg cctggtgtgc atccacgcgc
     9961 tgctgcgctg ggcgcgcgga cgcgagcccg ccggcgcggc gcggccgccc gaggttcgga
    10021 tcggcggcac gacctggtcg ggcctggccg agatcgcgcg ctcgccctac ctcgccgcca
    10081 tcgtggccta cgtgctgctc tacacggcgc tgctcgggtt cgcgtacttc gagctggcgc
    10141 ggatgatcgc cgagcgctac gccgatcccg cggagcgcac ggcgctgttc gcgcacgtcg
    10201 acctcgcggt gaacgtgctg acgctgctcg gccagctgct cgtcgtcggc cggctcgtcg
    10261 agcggctcgg cgtcggtgcg gcgctggcgc tcctgcccct gctcgcgctc gccgggtttt
    10321 ccgccatggg cgccgcaccc gtgctcgcgg tcatgctcgt gttccaggtg gcgcgccgcg
    10381 ccgccgacta cgcgatcgcg cggccggcgc gcgagatgct gttcaccgtg ctcgaccgca
    10441 acgccaagta caagtcgaag aacttcatcg acaccgtggt gttccgcggc ggcgacgccg
    10501 cgagcggctg gctgtacgcg ggattcaagg cgctggggct cgcgctgccc gggatggcgc
    10561 tcgccgcgat cccgggcgtg ctcgcctggc tcgcgctcgg gatcttcctc gggcgccgct
    10621 acgagggcct cgcccggcgc ggggtcgcaa cttcgggtcg ctagccgagc gcctcgcttt
    10681 tcctgcgcgc cggggcggcg acgacgcgcc gggcggggaa gcgcgcggcg aagcggctgc
    10741 cgcggccggg ctcgctcacg atctcgagcg ccccctggtg gcgcgcgagc gcgtgcttca
    10801 cgatcgcgag gccgaggccc gtgccgccgc tctcgcgcga gcggccccga tcgacccggt
    10861 agaagcgctc ggtgagccgc gggaggtgct cgcgctcgat gccgaggccc gtgtcctcca
    10921 cggcgaacgt ggcgccctcg ggcgaggcgc gccacacgag ccgcacctcg ccgcccgcgg
    10981 gcgtgtagcg gacggcgttg ctcgcgaggt tgccgaaggc gctcgcgagc tcgttctcgg
    11041 cgcccagaag atcgaaaccc tcctcggcgt ccaggcggac gcggtggcgc ccggccgaga
    11101 gcgcctgcgc ctcgccgagc acgcgcgcga gcagcagcgc gacgtcgacg cgctcgtcgt
    11161 gctcgggggc gggcgccgac tcgagcgtcg acagcgtgag caggtcgtcg atgatgcgct
    11221 gcatgcgccg gccctgctcg gccatgaggt tgagatagtc gcgcgagcgc tcgggatcga
    11281 gcttcagctc gcgcaccgtc tcgaggaagc cgatcagcac cgtgagcggc gtgcgcagct
    11341 cgtgcgacac gttggcgacg aagtcgcggc gcatggtctc gagccgcgtc gcctgcgtca
    11401 cgtcgcgcga cagcagcagc cattgcgact cgacgtaggg cacgagctgc accgagagga
    11461 tcaggccgtc gtcgcgcgcg gtcctgagcg cgagcggcgc cgagaagtcg cgcgccgcga
    11521 cgtagcgtac gaactcgggc tggcgcacga ggttgacgat cggccggccg atgtccgagc
    11581 gcacgtcgat gccgagatgg cgctcggagg agtcgttgca ccagaggatg cggtgctcgg
    11641 cgtcgaggat cgccatgccg tagggcagcg cgcgcccggc gcggcgcgag cgcgcgagca
    11701 gccgcgcgag cgcgcggcgc cggcgcagcc cggcgcgctc gcggcggtag agcagcgtca
    11761 gcgcttcctg ccacatcccc gtgccctcgg ggacgtccgg agcgaggccc gcggcccagc
    11821 gcgcgagcag ccgcaggtgg cgggcgtgcg agaggagcac cgccgcgagc ccgagggaga
    11881 agacggccca gccccaggcg gggccaaaga gcagcccggc ggcgagcgcg ggaaccgcga
    11941 ggagcgcgag cgcggcgagg gtgcgaacga gcggatcgtt cattccgcgc gcgcgctcat
    12001 ccgaaaagcg cgatcgccca gaccaccgcc acgttcacga gactgaggaa gaccgcggcg
    12061 ctgccgagat ccttcgcgcg cttggcgagg cgatgatgct ccggcgaggc caggtcgacc
    12121 acggcctcga gcgccgagtt gagcagctcg acgaccagca cgagcagcac gcacgcgatc
    12181 atcagcgccc tggcgggcgc ggagaccggc gcgagcagcg ccgcgggaat cagcacggcg
    12241 gcgaggagca gctcctggcg gaacgccgcc tcgtgccgca gcgccgacct cacgccctcg
    12301 agcgagtagc cgagcgcgtt gatgaggcgc cgcagtccgg tcttgccctt gaaggggctt
    12361 tcctgctcca tcgcgagcac cgcagggaaa aatgcgattt tacggggttc tcgggcgcct
    12421 gcgccccgcc ggcgtcgtcg cctggagcgc tccgctcgcg gtcgtcgcga gcattgcgag
    12481 cagctgctcc tgggccgagc gcggcagccg cccgcgcgag cgccgcaggc ggtagttgcc
    12541 gtcggcgtcc atctcccagg cctggctgtt gtcggagaga taaggccgca gtccctcgcg
    12601 gatcacgcgg ttcttgagct tcgggtcgag aaccggcacg cacagctcga tgcggcggaa
    12661 gaagttgcgc tccatccagt cggcgctcgc caggtagacg tcctcggcgc cgtcgttcag
    12721 gaagtagaag atccgcgagt gctcgaggaa gcgtcccacc accgagcgca cgcggatatt
    12781 gtccgagagg cccgggatgc cgggccgcag cgcgcacacg ccgcggatga cgaggtcgat
    12841 cttcgcgccg gcctgcgacg ccttgtacag cgcctcgatc accttgggtt cgaggagcgc
    12901 gttcatcttc gcgatgatgt gcgccggctt gccctcgcgc gcgtgcgccg cctcgttccg
    12961 gatcgccgcg agcacgcgct cgtgcagcgt gaacggcgag atccacaggt ggttcagccg
    13021 gcgcgcgcga ccaagaccgg tgagctgctt gaacacctcg ttcacgtcgt cgcagatgct
    13081 ctcgtccgcg gtgaacaggc cgaagtccgt atattgcttc gccgtgcgcg ggtggtagtt
    13141 cccggtcgag agatgcgcgt agcgcctgag cgtcccgttc tcgcgccgca tgaccagcga
    13201 catcttggcg tgcgtcttgt agccgaccac gccgtacacg acgtgcgcgc cgacctcctc
    13261 gagcttggcc gcccagttca ggttcgcctc ctcgtcgaag cgcgcgagca gctcgacgac
    13321 gacggtcacc tccttgccgc gctgcgccgc gtcgatgagg taccgcatga gcaccgaatc
    13381 cgtgccggtg cggtacacgg tctgcttgat cgcgaccacg tccgggtcct cgaccgactg
    13441 ctcgagaaag gcgatcacgg ggttgaacga ctggaacgga tggtgcagca ggatgtcggc
    13501 cttgcggatc gcgcggaaga gatcggcgcc cttgtcgagg ggccgcggca cgccgggcga
    13561 gaagggcggg tacttgagcc tcggcttgtc gacgagctcg ggaatctcca tgaggcggac
    13621 caggttcacc ggcccgtcga cgcggtagag atcgggcggc gcgagctcga actgctcgag
    13681 caggaactgc tccatcccgg ccggcatgtc ggcggcgatc tccaggcgca ccgagtcgcc
    13741 gaaatggcgc tgcggcagct cgccctgcag cgcgatgcgc atgttcttga cctcctcctc
    13801 gtcgagccag aggtcgctgt tgcgcgtcac gcggaacggg tacgagccga gcaccgacat
    13861 cccctggaac agcatgccga cgaacgcctg cagggcggac gtgagcagca cgaaggcgta
    13921 cgcgtcgccg gcgaggtccg cgggcaggcg gatcgcgcgc ggcagcaggc gcggcgcctg
    13981 cacgatcgcc ttgcccgagc tgcggccgaa ggcgtcggtg ccctcgagct ggacggcgaa
    14041 gttcaggctc ttgttgagca cgcgcgggaa cgggtgcgcg ggatcgagcc cgatcggggt
    14101 gagcagcggc atcatctcgc gcacgaagta ctcgcgcacc caggcgcgct gatcgggcgt
    14161 ccactgctgc tcggagagga acagcacgcc gtcgtcggcg agcgccggga ggacctcccg
    14221 gttcagcacg cgatactggt cgtccaccag cgcatgcgcg cgctcgctga tcgccgcgaa
    14281 gacctcgctc gccggcgtct cgccggtgat ctcgatctcc ttcccgagct tgatcttctc
    14341 cttcacttcg gcgacgcgga tctcgaagaa ctcgtcgaga ttgttcgaca cgatgcacag
    14401 gaagcgcagc cgctcgagcg tcggcacgcg gcggtcttcc gcctgcgcga gcacccgccg
    14461 gttgaactcg agcatgccga gctcgcgcgg caggaactgc gggcggacgc ggcgcgacgg
    14521 cagacggacg ggctgcagca tgggacgact ctaggaacgc tatgttgcgt ttttgttgcg
    14581 gtttgcggta acaatatcgt catgcgaatc gggcaagctg gcaacccgcg ttttccttcc
    14641 ggagccaagc gcttgatcgc gcaggaagtc tcgcggacgt gaagcccgat acgctggccg
    14701 cgctggacct cggctcgaac agcttccacc tgcagatcgc gcgcgtcctc gacgaccagg
    14761 tctacatgct cgacagcctg cgcgagcccg tccgcctcgg cggcgggctc acccgggacc
    14821 gcaggatcga ccggcgcacg cgcgcgcgcg cgatggcggc tctcgagcgc ttcgccgagc
    14881 ggctgcgggg ctttccgagg ggctcggtgc gcgcggtggg cacgagcgcg ctgcgcaccg
    14941 cgaagaacgc ctccgccttc atcgcgcagg ccgagcgcat cctcggttat cccgtggaag
    15001 tgatcttcgg acgcgaggag gcgcggctga tctatctcgg cgtcgcgcac gagctcgccg
    15061 cctcggacga gcggcggctc gtggtcgaca tcggcggcgg ctctaccgag tgcatcatcg
    15121 gcaaaggcct ggagccgcag ttgatggaaa gcctgcccat gggctgcgtc ggctacagcc
    15181 tgcgattctt cgccgaaggg cggctcgaca agacgcgctt cgagcgcgcc gagcgcgccg
    15241 cggcgaacga gcttcagcgc atcgtggacg cctaccgcgc cgccggctgg acgcacgcgg
    15301 tggcttcctc gggcacggcg cgggcgctcg catcgatcgt gcacggctgc gggctggccg
    15361 agcacggtct gaccgcgtcc gctctcgccg agctgcgcga ccgcttcatc agggccgggc
    15421 gcatcggccg cctgaagatt ccgggtctgc gcgaggaccg caagccgatc atcgcgggcg
    15481 gccttgcgat catgaccgcg gtgctggcgc aattcgggat cgacgcgctc gacgtctcgg
    15541 acgcggcgct ccgccagggc gtcatgtacg acctcctcgg gcgcgtgcgc caccgcgaca
    15601 tgcgcgaggc cacggtgcgc cagttcatgc gccgctacca ggtcgatagc gcgcaggccg
    15661 agcgcgtcgc cgcgcttgcg cagcgcgtcc tcgcggacat cgacccgaag gcctccgaaa
    15721 gcgacgcggc gctgctgcgc tgggcggcga gcctgcacga gatcggcatc tcgatcgcgc
    15781 acgcgggcta ccacaagcac tcggcctaca tcgtctcgca ggccgacatg ccggggttct
    15841 cgcaatcgga gcaggcgcgc ctcgcgcgcc tggtgctcg
//
LOCUS       DATDMW010000410         3875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_123964, whole genome shotgun sequence.
ACCESSION   DATDMW010000410 DATDMW010000000
VERSION     DATDMW010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123964"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VI319_17035"
     CDS             <1..679
                     /locus_tag="VI319_17035"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEY6865600.1"
                     /translation="GGTALESDRALDARAAERLRHRARAIDAWDYERIVLQAFPEIRK
                     VKTVPHCAAQGGFLDPGHVTLVVVPDLRLRNAVDPLQPRADADTLRRVREWVEARAPM
                     RIGVHVRNPRFQRIRLDFKVRFRPGVEFNYHARLLREALIDHLCPWRHDPGAAIAFGG
                     TVYKSQLLDFVEDTDTVDYVTDFRMHDLRGGTADAEDVEEARALTPDTILVSDASHDI
                     APVPDAP"
     gene            676..3225
                     /locus_tag="VI319_17040"
     CDS             676..3225
                     /locus_tag="VI319_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865601.1"
                     /translation="MSLPAVPVVRKPGLAPLGLDQQPLFDLGMRAVRAYASEVWTDHN
                     VHDPGVTTLELLCYALTDLCYRACYPVEDLLALERGNRANMAGQFYTVRQALPNAPLT
                     ELDTRKLLIDVDGVRNAWILAADADPIYVDPVAGELLAADPGTPDIRTVHLRGLYRAL
                     IELQNPDAAPADRARVVAAAGAALQSHRNLCQDFTGVDVVDTQAFLLCAEIEIEPQAD
                     PSETYARILVAVQEHLAPGVPRRTRDEMLARPLPDGGARGDPDLYEGPALAGGFIDDD
                     ELAAAELRVTIRLSDVIALVMDVEGVRAVREIVLRPADPDEAEATKDDSKWEVRVDPG
                     RRSVLDAEHSRLVLYKSGMPLPRGADVMTRYQALKDQAEARFQPRAMGDPAIPLGRWR
                     AAADYASVQRDFPDVYGIGDAALPPDAGPERRAQALQLEGYLLFFDQWLANAFAQLGG
                     VRDLFSRDPEVVRTYFAQPVAGMDALYRPGAGGVELESAADAIARRNRFLDHLVARFA
                     ERLPDDLQVQAALFGATPAAIARAKCAFLADVPRLCADRGRAFDYTVDPASSAHAANV
                     SGLEKRLAHLLGLGSLAREIYQEQDTDGIDEYRWRVRRRFADGVLLSAEAHYATPDLA
                     DAAMAAAIAAAARASGYQRRQTGPGRFYFNVVDAAGHVIGRRIEYFATAAARDTAIDE
                     LIDLVGQDQGERMILVENILLRPRPGAAGTFLPICAEPACAGGCPGDDPYSYRLHVVL
                     PALAPRFRNMEFRRYAEEVIREETPAHLLAKICWVGDDDMKKVEAAWGAWQAVLAGTD
                     ATGAAAKLAALRDALFGAKNVYPPSTLADCAAPEKFVLGRSALGSEPPAPA"
     gene            3248..>3875
                     /locus_tag="VI319_17045"
     CDS             3248..>3875
                     /locus_tag="VI319_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865602.1"
                     /translation="MTTEASLSLDTIETAFTVFETDQVLTADQLNGVADYLDDQQRLT
                     RVALIGVGIACGLWPSLQSGAVRLSGGVGTTTDGDVLFFPDAAVYDHYKSYDATTPRY
                     APFASGDGAITAYELLTAAQADGDARAEPLSGFDAAESPRALAGMVAVLYVESYLHDP
                     DLCTATDCDNRGKNWQHTTRLLLVDAASAAALRETLDTPDAAARALAPV"
BASE COUNT          536 a         1493 c         1342 g          504 t
ORIGIN      
        1 cggcggcacg gcgctcgagt ccgaccgcgc gctcgacgcg cgcgccgcgg agcggctgcg
       61 ccaccgcgcg cgcgcgatcg acgcctggga ctacgagcgc atcgtgctcc aggccttccc
      121 cgagatccgc aaggtcaaga ccgtgccgca ctgcgcggcg cagggcggct tcctcgatcc
      181 ggggcacgtg acgctcgtcg tcgtgcccga cctgcggctg cgcaacgcgg tcgacccgct
      241 gcagccgcgc gccgacgccg acaccctgcg ccgcgtccgc gagtgggtcg aggcgcgcgc
      301 gcccatgcgc atcggcgtgc acgtgcgcaa cccgcgcttc cagaggatcc gcctggactt
      361 caaggtgcgc ttccggcccg gcgtcgagtt caactaccac gcgcgcctgc tgcgcgaggc
      421 gctgatcgac cacctgtgcc cctggcgcca cgaccccggc gcggcgatcg cgttcggcgg
      481 cacggtgtac aaatcgcagc tgctcgattt cgtcgaagac accgacaccg tcgactacgt
      541 caccgacttc cgcatgcacg acctgcgcgg cgggacggcc gatgccgagg acgtcgagga
      601 ggcgcgcgcg ctgacgcccg acaccatcct cgtttccgac gcgagccacg acatcgcgcc
      661 cgtgcccgac gccccatgag cctgcccgcc gtccccgtcg tgcgcaagcc cggcctcgcc
      721 ccgctcgggc tcgaccagca gccgctgttc gatctcggga tgcgcgcggt gcgcgcctac
      781 gcgagcgagg tctggaccga ccacaacgtg cacgatccgg gcgtgacgac gctcgagctg
      841 ctgtgctacg cgctcaccga cctgtgctac cgcgcctgct acccggtcga ggacctgctc
      901 gccctcgagc gcgggaacag ggcgaacatg gcgggccagt tctacaccgt gcgccaggcg
      961 ctgccgaacg cgccgctcac cgagctcgac acccgcaagc tgctgatcga cgtcgacggc
     1021 gtgcgcaacg cctggatcct ggccgccgac gccgacccga tctacgtcga ccccgtcgcc
     1081 ggcgagctgc tcgccgcgga tccgggaacg ccagacatcc gcacggtgca tctgcgcggc
     1141 ctctaccgcg cgctgatcga gctgcagaac ccggacgcgg cgcccgccga ccgcgcgcgc
     1201 gtcgtcgccg cggccggcgc ggcgcttcag tcgcaccgca acctgtgcca ggacttcacc
     1261 ggcgtcgacg tcgtcgacac gcaggccttc ctgctctgcg ccgagatcga gatcgagccg
     1321 caggccgacc cgagcgagac ctacgcgagg atcctggtcg cggtgcagga gcacctcgcg
     1381 ccgggcgtgc cgcggcgcac gcgcgacgag atgctcgcgc gcccgcttcc cgacggcgga
     1441 gcgcgcggcg atcccgacct ctacgagggg ccggcgctcg ccggcggctt catcgacgac
     1501 gacgagctcg ccgcagccga gctgcgcgtg acgatccggc tctcggacgt gatcgcgctc
     1561 gtgatggacg tcgagggcgt gcgcgcggtg cgcgagatcg tgctgcggcc cgccgatccc
     1621 gacgaggccg aggcgacgaa ggacgacagc aagtgggagg tgcgcgtcga tcccggccgg
     1681 cgctcggtgc tcgacgcgga gcattcgcgc ctggtcctgt acaagagcgg catgccgctg
     1741 ccgcgcggcg ccgacgtcat gacgcgctac caggcgctga aggaccaggc cgaagcccgg
     1801 ttccagccga gggctatggg cgatcccgcg atcccgctcg ggcgctggcg cgcggcggcc
     1861 gactacgcgt cggtgcagcg ggatttcccc gacgtgtacg gcatcggcga cgcggcgctg
     1921 ccgcccgacg ccgggcccga gcgtcgggcc caggcgctgc agctcgaagg ctatctgctt
     1981 ttcttcgacc agtggctcgc gaacgccttc gcgcagctcg gcggcgtgcg cgacctgttc
     2041 tcgcgcgacc ccgaggtcgt gcgcacgtat ttcgcgcagc cggtggcggg gatggatgcg
     2101 ctgtatcggc cgggggcggg aggcgtggag ctcgagagcg ccgccgacgc gatcgcgagg
     2161 cgcaaccgct ttctcgatca cctcgtcgcc cgcttcgccg agcgcctccc cgacgatctc
     2221 caggtccagg ccgcgctgtt cggcgccacg ccggccgcga tcgctcgcgc gaagtgcgcg
     2281 ttcctcgccg acgtgccgcg cctttgcgcg gatcgcggcc gcgcgttcga ctacacggtc
     2341 gaccctgcgt cgagcgcgca cgccgccaac gtctccggcc tcgagaagcg cctcgcgcac
     2401 ctgctcggcc tcgggagcct cgcgcgcgag atctaccagg agcaggacac cgacggcatc
     2461 gacgagtacc gctggcgcgt gcgccggcgc ttcgccgacg gcgtgctgct cagcgccgag
     2521 gcgcactacg ccacgccgga tctcgccgac gccgccatgg ccgccgcgat cgccgcggcg
     2581 gcgcgggcct cgggctacca gcgccgccag acgggccccg gcaggttcta cttcaacgtc
     2641 gtcgacgcgg ccggccacgt catcggccgg cgcatcgagt acttcgcgac cgcggcggcg
     2701 cgcgacacgg cgatcgacga gctgatcgat ctcgtcggcc aggaccaagg cgagcgcatg
     2761 atcctggtcg agaacatcct gctgcggccg cgtcccggcg ccgccggcac gttcctgccg
     2821 atctgcgccg agcccgcgtg cgccggcggc tgccccggcg acgacccgta ctcgtaccgg
     2881 ctgcacgtcg tgctgcccgc gctcgcgccg cgcttccgca acatggagtt ccgccgctac
     2941 gccgaggagg tgatccgcga ggagaccccg gcgcacctgc tggcgaagat ctgctgggtc
     3001 ggcgacgacg acatgaagaa ggtcgaggcc gcgtggggcg cctggcaggc ggtgctcgcc
     3061 ggcaccgatg cgaccggcgc ggccgcgaag ctcgccgcgc tcagagacgc gctcttcggc
     3121 gcgaagaacg tctacccgcc ctcgacgctg gccgactgcg ccgcgcccga gaaattcgtc
     3181 ctcgggcgca gcgcgctcgg cagcgagccg ccggccccgg cgtgaacgca catccaagga
     3241 gcgtcccatg acgaccgaag catcgctcag cctcgacacc atcgagaccg cgttcacggt
     3301 cttcgagacc gaccaggtcc tgaccgccga ccagctgaac ggcgtcgccg actacctgga
     3361 cgaccagcag cgattgacgc gggtcgcgct catcggcgtc ggcatcgcgt gcgggctgtg
     3421 gccctcgctg caatcgggag cggtgcggct ctcgggcggc gtcggcacca cgaccgacgg
     3481 cgacgtcctc ttcttccccg acgccgcggt gtacgaccac tacaagagct acgacgcgac
     3541 cacgccccgg tacgcgccgt tcgcctcggg cgacggcgcg atcaccgcgt acgagctgct
     3601 caccgcggcg caggccgacg gcgacgcgcg cgccgagccg ctctcgggct tcgacgccgc
     3661 ggagtcgccg cgcgcgctcg ccggcatggt ggctgtgctc tacgtcgaga gctacctgca
     3721 cgatccggac ctgtgcaccg cgaccgactg cgacaaccgc ggcaagaact ggcagcacac
     3781 gacgcgcctg ctgctggtcg acgcggcctc cgccgcggcg ctgcgcgaga cgctcgacac
     3841 gcccgacgcc gcggcgcgcg cgctcgctcc cgtgc
//
LOCUS       DATDMW010000411         2944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U421
            k141_1244514, whole genome shotgun sequence.
ACCESSION   DATDMW010000411 DATDMW010000000
VERSION     DATDMW010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962621
            Sequence Read Archive: SRR25158281
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,412
            CDSs (total)                      :: 3,372
            Genes (coding)                    :: 3,349
            CDSs (with protein)               :: 3,349
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244514"
                     /isolate="SMAG_U421"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /gene="lgt"
                     /locus_tag="VI319_17050"
     CDS             complement(<1..637)
                     /gene="lgt"
                     /locus_tag="VI319_17050"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY6865603.1"
                     /translation="MLVHPNFDPIAFSIGPLSVRWYGLMYLVGFGIAYTLGRVRIAKA
                     RPATISVAALDDLLFFCVLGVVLGGRLGYVLFYKPGYYFSHPLEIFAVWQGGMSFHGG
                     FLGVVAALAYVARKHRLRVLEMFDFFVPLAPLALAAGRLGNFINGELYGRVTDVPWGM
                     VFQSPGAGPLPRHPSQLYQFSLEGVLLFVILWIYSAKPRPVGAVSAAFLVGY"
     gene            698..2371
                     /gene="ilvD"
                     /locus_tag="VI319_17055"
     CDS             698..2371
                     /gene="ilvD"
                     /locus_tag="VI319_17055"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY6865604.1"
                     /translation="MADNWRSKLITQGPARSPNRAMLRAVGFGDGDFDKPIVGVANGH
                     STLNPCNAGIQPIVDRAVAALHEAGAMPQMFGFPTVTDGIGMGTEAMKYSLVSREVIA
                     DAIETSVNGQAMDGVLAVGGCDKNMPGCMMAIARMDVPAIYVYGGTIKPGKWKGQDLT
                     IVSAFEAVGAYSAGKMSEEDFVGIEKNACPTVGACGGMYTANTMSSSFEALGMSLLGS
                     SQMASPDPEKADSAAESAQVLVQAVRKGLRPRQIITRKSIENAISLVMATGGSTNAVL
                     HYLAIARAAGVKWTIDDFERVRRKVPVLCDLKPSGRYVATDFHRAGGVPAVLKILLEH
                     GLLDGDCMTITGRSLGEELASVPPAPVGQQVIRPFDKPMYKQGHLAILKGNLATEGCV
                     AKITGLKQTSITGPARVFDSEPAAMKAILAKRIKAGDVIVIRYEGPKGGPGMQEMLAP
                     TSALIGQGLGDSVGLITDGRFSGGTWGMVVGHVAPEAYVGGTIALVKEGDSITIDAGK
                     RLIQLNVSAKELAARRKKWKQPKPRYTSGLLAKYMKLVSTASEGAVTDR"
     gene            complement(2375..>2944)
                     /locus_tag="VI319_17060"
     CDS             complement(2375..>2944)
                     /locus_tag="VI319_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6865605.1"
                     /translation="QTTTEGMRQVRGEVTVNGTPVRVGDAVRPGDTVATGSRSFAAFV
                     VGRDAFLVRDSTRVEIAGGAGADLLRLITGKLLGVFGKGEGRRLVTANATIGIRGTGA
                     YLEAEAVRTYFCLCYGTADVATADGKVKDSYSTTHHESPRYLYGDGRRDAMTSARVVN
                     HTDAELIMLEALVGRQPPREVMDSRWPRY"
BASE COUNT          522 a          972 c         1061 g          389 t
ORIGIN      
        1 cgtacccgac caggaacgcc gccgataccg cccccacggg ccgcggcttc gccgagtaga
       61 tccacaggat cacgaagagc agcacgccct cgaggctgaa ctggtagagc tgcgagggat
      121 ggcgcggcag cgggcccgcg cccggcgact ggaacaccat gccccagggt acgtcggtca
      181 cccggccgta gagctcgccg ttgatgaagt tgccgaggcg gccggcggcg agggcgagcg
      241 gggcgagcgg gacgaagaag tcgaacatct cgagcacgcg cagccggtgc ttgcgcgcga
      301 cgtaggcgag cgcggcgacc acgccgagga agccgccgtg gaacgacatc ccgccctgcc
      361 acacggcgaa gatctcgagc ggatgcgaga agtagtagcc gggtttgtag aacaggacgt
      421 agccgaggcg accgccgaga accaccccca gcacgcagaa gaaaagcagg tcgtcgagcg
      481 cggccacgct gatggtcgcc ggccgcgcct tcgcgatgcg cacgcgcccg agcgtatagg
      541 cgatgccgaa gccgacgagg tacatgagcc cgtaccagcg cacgctcagg gggccgatcg
      601 agaatgcgat cgggtcgaag ttgggatgga cgagcacggg gcctcggtcg gtgattcggt
      661 acaatcggca gaggggagta taacgagaga acaagccatg gcggacaact ggagaagcaa
      721 gctcatcacc cagggcccgg cgcgctcgcc caaccgcgcg atgctgcgcg cggtcggctt
      781 cggcgacggc gatttcgaca agcccatcgt cggcgtcgcg aacggccact cgacgctcaa
      841 cccctgcaac gccggcatcc agccgatcgt cgatcgcgcg gtcgcggcgc tgcacgaggc
      901 gggggcgatg ccgcagatgt tcggcttccc gaccgtcacc gacggcatcg gcatgggcac
      961 cgaggcgatg aagtactcgc tcgtctcgcg cgaggtgatc gccgacgcga tcgagacctc
     1021 ggtcaacggg caggcgatgg acggcgtgct cgcggtcggc ggctgcgaca agaacatgcc
     1081 cggctgcatg atggcgatcg cgcgcatgga cgtgcccgcg atctacgtct acggcggcac
     1141 gatcaagccc ggcaagtgga aaggtcagga cctcaccatc gtcagcgcct tcgaggcggt
     1201 cggcgcgtac tccgccggca agatgagcga ggaggacttc gtcggcatcg agaagaacgc
     1261 gtgcccgacg gtcggcgcgt gcggcggcat gtacaccgcg aacacgatgt cgtcgtcgtt
     1321 cgaggcgctc ggcatgagcc tgctcgggtc gtcgcagatg gcgtcgcccg atccggagaa
     1381 ggcggactcc gccgccgagt cggcgcaggt gctggtgcag gcggtgcgca agggcctgcg
     1441 tccgcggcag atcatcacgc gcaagtcgat cgagaacgcg atcagcctgg tcatggcgac
     1501 gggcgggtcg accaacgcgg tgctgcacta cctggcgatc gcgcgcgcgg ccggcgtcaa
     1561 gtggacgatc gacgacttcg agcgcgtgcg ccgcaaggtg ccggtgctgt gcgacctgaa
     1621 accctcggga cgctacgtcg cgaccgactt ccaccgcgcg ggcggcgtgc cggcggtgct
     1681 gaagatcctg ctcgagcacg ggctgctcga cggcgattgc atgacgatca cggggcgcag
     1741 cctcggcgag gagctcgcga gcgtgccgcc cgcgccggtc gggcagcagg tgatccgtcc
     1801 cttcgacaag ccgatgtaca agcaggggca cctcgccatc ctcaagggca acctcgccac
     1861 cgagggctgc gtcgccaaga tcacgggcct gaagcagaca tcgatcacgg gacccgcacg
     1921 cgtgttcgac tccgagcccg cggcgatgaa ggcgatcctc gcgaagagaa tcaaggcggg
     1981 cgacgtgatc gtcatccgct acgagggccc gaagggcggg cccgggatgc aggagatgct
     2041 cgcgccgacg tcggcgctga tcgggcaggg actcggcgac tcggtcggcc tgatcaccga
     2101 cggccgtttc tccggcggca cctggggcat ggtcgtgggc cacgtcgcgc ccgaggcgta
     2161 cgtcggcggc acgatcgcgc tcgtgaagga aggcgactcg atcacgatcg acgcgggcaa
     2221 gcgcctcatc cagctcaacg tctccgcgaa ggagctggcc gcgcgccgca agaaatggaa
     2281 gcagccgaag ccgcgctaca cgagcggcct gctcgcgaaa tacatgaagc tcgtgtcgac
     2341 ggctagcgag ggcgcggtca ccgatcgctg aaacctaata acggggccag cgcgagtcca
     2401 tcacctcgcg tggcggctgg cggccgacca gcgcctcgag catgatcagc tcggcgtcgg
     2461 tatggttcac gacgcgcgcc gacgtcatcg cgtccctgcg cccgtcgccg tagaggtagc
     2521 gcggcgactc gtggtgcgtg gtcgagtagc tgtccttcac cttcccgtcg gcggtcgcga
     2581 cgtccgcggt cccatagcac aggcagaagt aggtgcgcac cgcttcggcc tcgaggtacg
     2641 cgcccgtgcc gcggatgccg atggtggcgt tcgcggtgac gagcctgcgg ccctcgccct
     2701 tgccgaagac gccgaggagc ttcccggtga tgaggcgcaa aaggtcggcg cccgcgccgc
     2761 ccgcgatctc gacgcgcgtc gagtcgcgca cgagaaaggc gtcgcgcccg acgacgaagg
     2821 cggcgaagga gcgcgatccc gtcgccaccg tgtcgcccgg gcgcacggcg tcgccgacgc
     2881 gcaccggcgt gccgttgacg gtgacctcgc cgcggacctg gcgcatgcct tcggtggtgg
     2941 tctg
//
